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Sample records for carcass microbiological evaluation

  1. Microbiological evaluation of chicken carcasses in an immersion chilling system with water renewal at 8 and 16 hours.

    Science.gov (United States)

    Souza, L C T; Pereira, J G; Spina, T L B; Izidoro, T B; Oliveira, A C; Pinto, J P A N

    2012-05-01

    Since 2004, Brazil has been the leading exporter of chicken. Because of the importance of this sector in the Brazilian economy, food safety must be ensured by control and monitoring of the production stages susceptible to contamination, such as the chilling process. The goal of this study was to evaluate changes in microbial levels on chicken carcasses and in chilling water after immersion in a chilling system for 8 and 16 h during commercial processing. An objective of the study was to encourage discussion regarding the Brazilian Ministry of Agriculture Livestock and Food Supply regulation that requires chicken processors to completely empty, clean, and disinfect each tank of the chilling system after every 8-h shift. Before and after immersion chilling, carcasses were collected and analyzed for mesophilic bacteria, Enterobacteriaceae, coliforms, and Escherichia coli. Samples of water from the chilling system were also analyzed for residual free chlorine. The results do not support required emptying of the chiller tank after 8 h; these tanks could be emptied after 16 h. The results for all carcasses tested at the 8- and 16-h time points indicated no significant differences in the microbiological indicators evaluated. These data provide both technical and scientific support for discussing changes in federal law regarding the management of immersion chilling water systems used as part of the poultry processing line.

  2. Microbiological analysis of dried goose carcasses

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    Ufuk Kamber

    2016-10-01

    Full Text Available Microbiological studies on chicken meat and carcasses are well documented, but very few studies exist on goose meat and carcasses. Therefore, in this study, dried goose carcass samples were collected from the local households in Kars/Turkey and microbiologically analyzed in terms of public health risks. The total mesophilic viable count was found to be 6.58 (mean log10 CFU g-1 (100%. The number of Enterobacteriaceae was 4.85 (92.8%. Coliform bacteria was counted at the numbers of 2.98 (67.8%, while it was 3.95 (91.1% for the enterococci, 0.42 (26.7% for the clostridia, 0.04 (3.5% for the Clostridium perfringens, and 0.41 (12.5% for the coagulase positive staphylococci. The numbers of mould and yeast were 0.93 (25% and 4.81 (94.6%, respectively. Salmonellae and Bacillus cereus could not be isolated in the samples. The results indicate that the dried goose meat samples had poor hygienic quality, contained some of the pathogen microorganisms that are likely to pose a potential health risk.

  3. Effect of fecal material on carcass microbiology

    Science.gov (United States)

    Broiler carcass bacterial counts are increased by visible fecal material, therefore further treatment of these carcasses is necessary. Inside-outside bird washers (IOBWs) are used by many processors to remove feces and possibly reduce bacterial counts. A series of studies have shown that IOBWs hav...

  4. The impact of cooling methods on microbiological quality of broiler carcasses

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    Peter Popelka

    2014-02-01

    Full Text Available The aim of this work was to compare two chilling methods, combined (aerosol and water chilling, in terms of their effectiveness in chilling of different weight categories of broiler chickens. At the same time microbial associations of different weight categories of broiler chickens were evaluated. Samples were collected in an approved establishment and poultry carcasses were divided according to weight and chilling methods into five categories. The first four categories were chilled using combined chilling method and fifth category was chilled with water. The temperature of the breast muscle before and after chilling and microbiological parameters (total viable count, Enterobacteriaceae, Salmonella was measured. By comparing the temperature of the breast muscle after combined chilling method was not achieved in the breast muscles temperature below 4 °C in all weight categories. In any case, the lowest average temperature has been reached in the weight category <1.2 kg (4.9 °C and with increasing weight, the average temperature was rising, and the highest was in weight category 1.8 to 2.5 kg (10.8 °C. Poultry carcasses were subsequently divided into portions and after cutting were chilled up to a temperature below 4 °C. In poultry carcasses chilled by water, the average temperature of the breast muscle after 20 minutes in the water bath was even higher (19.6 °C compared to combine chilling. Thus chilled poultry carcasses were frozen up to -18 °C in a core of muscles. Comparing the microbiological contamination in different weight categories and chilling techniques, we found that the lowest total viable count (TVC before and after chilling was in the lowest category and the difference before chilling was significantly lower comparing with all other categories. Conversely TVC after chilling by water was decreased. In comparing the number of Enterobacteriaceae before and after chilling, a similar pattern of contamination as above was found

  5. Microbiological contamination of lamb carcasses at abattoirs of Istanbul

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    Tolga Kahraman

    2005-01-01

    Full Text Available A total of a hundred lamb carcasses were sampled over a 12 month period at abattoirs in Istanbul, Turkey. Each sample examined for total aerobic mesophilic counts (TMC, Enterobacteriaceae count (EC, Salmonella spp. Escherichia coli O157:H7 and Listeria monocytogenes was obtained from 100 cm2 areas on four sides of lamb carcasses using the wet and dry cotton swab technique. The study revealed that total aerobic mesophilic counts in all carcasses ranged between 4.18 and 5.95 log/cm2; Enterobacteriaceae counts between 1.60 and 2.30 CFU/cm2. All samples were negative for Escherichia coli O157:H7 and Listeria monocytogenes. Furthermore Salmonella spp. was detected on four carcasses. The data confirms bacteriological monitoring of lamb carcasses as a useful criteria for the verification of slaughter hygiene.

  6. Scalding: Effects of Additives on Carcass Microbiology and Wastewater Discharge

    Science.gov (United States)

    Scalding poultry before removing feathers has been practiced for thousands of years, but spoilage and pathogenic bacteria were discovered relatively recently by comparison. The microbiological consequences of scalding and other practices used in converting live birds to food was unknown, although i...

  7. Procedures for evaluating pork carcass and cut composition

    International Nuclear Information System (INIS)

    Five studies were completed to investigate various production and evaluation procedures related to pork carcass composition and meat quality. A comparison of market hog characteristics of pigs selected by feeder pig frame size or current USDA feeder pig standards was made. In general, feeder pig frame size did differentiate between carcass skeletal traits (i.e., carcass length, radius length). However, frame sizing did not improve on current feeder pig grades in discriminating between carcass composition characteristics. Liquid scintillation of potassium-40 was used to estimate pork carcass composition of 124 boars barrows and gilts, ranging from 23 to 114 kg live weight. Pigs were counted live, slaughtered and one side of the carcass was counted. The side was then ground and sampled for percent protein, fat and moisture. Carcass weight and 40K determined potassium of the carcass explain more of the variation in carcass composition than live animal traits. Carcass measurements were used to determine value and percentages of fat standardized lean, protein, fat and moisture in the carcass using 265 barrow and gilt carcasses. In a separate study, belly composition was estimated from carcass and belly parameters (n = 338). Ribbed carcasses measurements were almost always superior to unribbed carcass measurements when estimating carcass or belly composition. Tenth rib fat depth was the most useful single variable for predicting belly fat, protein, moisture and lean. Some precision and accuracy were lost when using parameters from unribbed carcasses to estimate carcass or belly composition as compared to including parameters from ribbed carcasses. The sensory and nutritive value of cooked pork center loin chops and roasts were investigated. Levels of fat cover and internal temperature did not greatly affect cholesterol content

  8. Evaluation of Simmental carcass EPD estimated using live and carcass data.

    Science.gov (United States)

    Crews, D H; Pollak, E J; Quaas, R L

    2004-03-01

    This study was conducted to compare carcass EPD predicted using yearling live animal data and/or progeny carcass data, and to quantify the association between the carcass phenotype of progeny and the sire EPD. The live data model (L) included scan weight, ultrasound fat thickness, longissimus muscle area, and percentage of intramuscular fat from yearling (369 d of age) Simmental bulls and heifers. The carcass data model (C) included hot carcass weight, fat thickness, longissimus muscle area, and marbling score from Simmental-sired steers and cull heifers (453 d of age). The combined data model (F) included live animal and carcass data as separate but correlated traits. All data and pedigree information on 39,566 animals were obtained from the American Simmental Association, and all EPD were predicted using animal model procedures. The genetic model included fixed effects of contemporary group and a linear covariate for age at measurement, and a random animal genetic effect. The EPD from L had smaller variance and range than those from either C or F. Further, EPD from F had highest average accuracy. Correlations indicated that evaluations from C and F were most similar, and L would significantly (P carcass data. Progeny (n = 824) with carcass data collected subsequent to evaluation were used to quantify the association between progeny phenotype and sire EPD using a model including contemporary group, and linear regressions for age at slaughter and the appropriate sire EPD. The regression coefficient was generally improved for sire EPD from L when genetic regression was used to scale EPD to the appropriate carcass trait basis. The EPD from C and F had similar linear associations with progeny phenotype, although EPD from F may be considered optimal because of increased accuracy. These data suggest that carcass EPD based on a combination of live and carcass data predict differences in progeny phenotype at or near theoretical expectation. PMID:15032422

  9. The Effect of Water Pressure and Chlorine Concentration on Microbiological Characteristics of Spray Washed Broiler Carcasses

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    Pissol AD

    2013-08-01

    Full Text Available The objective of this study was to evaluate the efficiency of water pressure and concentration of dichloromethane after the evisceration system under the fecal decontamination of chicken carcasse  surfaces with and without apparent contamination. From a total of  322 carcasses, 50% were intentionally added chicken droppings in an area of more  than 2 cm2 and the rest of carcasses were kept without fecal inoculation. Escherichia coli and Enterobacteriaceae counting was carried out in samples immediately after the inoculation (initial counting and after different treatments. Treatments consisted of water with different pressures (1.5,  3.5 and  5.5 Kgf/cm2, and the addition of a echnological adjuvant (dichloride at the concentrations of 0, 5 and 10 ppm. The results were validated using  40 chicken carcasses for each treatment by means of a  22  factorial statistical design. The results showed no significant differences (P

  10. Salmonella Prevalence and Microbiological Contamination of Pig Carcasses and Slaughterhouse Environment

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    Fois, Federica; Mazza, Roberta; Putzolu, Miriam; Delogu, Maria Luisa; Lochi, Pier Giorgio; Pani, Sergio Pino; Mazzette, Rina

    2014-01-01

    In seven EC swine abattoirs Salmonella prevalence (ISO 6579/2002) and serotypes of 25 piglets, 61 finishing pigs (lymph nodes, colon content, carcass and liver surface) and slaughterhouse environments (scalding water, surfaces in contact with meat and not in contact with meat) were investigated. Moreover, aerobic colony count [total viable count (TVC); ISO 4833] and Enterobacteriaceae (ISO 21528-2) of piglets and finishing pigs’ carcasses were evaluated, and the results compared with EU process hygiene criteria (Reg. EC 2073/2005). Salmonella was not isolated in any of the piglets samples. Prevalence differed between slaughterhouses (PSalmonella was isolated from 39 of 244 samples of finishing slaughtered pigs (15.9%) and from 4 of 45 environmental samples (8.9%). In pig samples, carcasses showed the highest prevalence (18%) followed by colon content (14.8%), lymph nodes (13%) and liver (1.6%). S. Anatum was the most prevalent serotype (71.8%), followed by S. Derby (33.3%), S. Bredeney (5%) and S. Holcomb (2.5%). Between environmental samples, S. Anatum (50%), S. Bredeney and S. Derby (25%) were identified. Total viable mean counts (log10 CFU/cm2) of carcass surfaces ranged from 4.6 and 5.7 for piglets, and from 4.6 and 5.9 for finishing pigs, while Enterobacteriaceae ranged between 1.1 and 5 for piglets and between 2.1 and 5.3 for finishing pigs. These results were not in compliance with EU performance criteria. Total aerobic viable counts and Enterobacteriaceae mean levels of environmental samples appeared critical, particularly referred to surfaces in contact with meat (splitting equipment) and indicated an inadequate application of good manufacturing and hygiene practices during slaughtering and sanitisation.

  11. Salmonella prevalence and microbiological contamination of pig carcasses and slaughterhouse environment

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    Francesca Piras

    2014-12-01

    Full Text Available In seven EC swine abattoirs Salmonella prevalence (ISO 6579/2002 and serotypes of 25 piglets, 61 finishing pigs (lymph nodes, colon content, carcass and liver surface and slaughterhouse environments (scalding water, surfaces in contact with meat and not in contact with meat were investigated. Moreover, aerobic colony count [total viable count (TVC; ISO 4833] and Enterobacteriaceae (ISO 21528-2 of piglets and finishing pigs’ carcasses were evaluated, and the results compared with EU process hygiene criteria (Reg. EC 2073/2005. Salmonella was not isolated in any of the piglets samples. Prevalence differed between slaughterhouses (P<0.5, and Salmonella was isolated from 39 of 244 samples of finishing slaughtered pigs (15.9% and from 4 of 45 environmental samples (8.9%. In pig samples, carcasses showed the highest prevalence (18% followed by colon content (14.8%, lymph nodes (13% and liver (1.6%. S. Anatum was the most prevalent serotype (71.8%, followed by S. Derby (33.3%, S. Bredeney (5% and S. Holcomb (2.5%. Between environmental samples, S. Anatum (50%, S. Bredeney and S. Derby (25% were identified. Total viable mean counts (log10 CFU/cm2 of carcass surfaces ranged from 4.6 and 5.7 for piglets, and from 4.6 and 5.9 for finishing pigs, while Enterobacteriaceae ranged between 1.1 and 5 for piglets and between 2.1 and 5.3 for finishing pigs. These results were not in compliance with EU performance criteria. Total aerobic viable counts and Enterobacteriaceae mean levels of environmental samples appeared critical, particularly referred to surfaces in contact with meat (splitting equipment and indicated an inadequate application of good manufacturing and hygiene practices during slaughtering and sanitisation.

  12. Microbiology of broiler carcasses and chemistry of chiller water as affected by water reuse.

    Science.gov (United States)

    Northcutt, J K; Smith, D; Huezo, R I; Ingram, K D

    2008-07-01

    A study was conducted to determine the effects of treating and reusing poultry chiller water in a commercial poultry processing facility. Broiler carcasses and chiller water were obtained from a commercial processing facility which had recently installed a TOMCO Pathogen Management System to recycle water in sections 2 and 3 of two 3-compartment chillers. In this system, reused water is blended with fresh water to maintain the chiller volume. Carcasses were sampled prechill and postchill (final exit), and chiller water was sampled from the beginning and end of each of the 3 sections. Carcasses were subjected to a whole carcass rinse (WCR) in 0.1% peptone. Numbers of Escherichia coli (EC), coliforms (CF), and Campylobacter (CPY) were determined from the WCR and chiller water samples. Prevalence of Salmonella (SAL) was also determined on the WCR and chiller water samples. On average, prechill levels of bacteria recovered from rinses were 2.6, 2.9, and 2.6 log10 cfu/mL for EC, CF, and CPY, respectively. Ten out of 40 (25%) prechill carcasses were positive for SAL. After chilling, numbers of EC, CF, and CPY recovered from carcass rinses decreased by 1.5, 1.5, and 2.0 log10 cfu/mL, respectively. However, 9 out of 40 (22%) postchill carcasses were positive for SAL. When the chiller water samples were tested, counts of EC, CF, and CPY were found only in water collected from the first section of the chiller (inlet and outlet). Two of 4 water samples collected from the inlet of the first section tested positive for SAL. This study shows that fresh and reused water can be used to cool poultry in chiller systems to achieve a reduction in numbers of bacteria (EC, CF, and CPY) or equivalent prevalence (SAL) of bacteria recovered from broiler carcasses.

  13. Microbiology of Broiler Carcasses and Chemistry of Chiller Water as Affected by Water Reuse

    Science.gov (United States)

    A study was conducted to determine the effects of treating and reusing poultry chiller water in a commercial poultry processing facility. Broiler carcasses and chiller water were obtained from a commercial processing facility which had recently installed a TOMCO Pathogen Management SystemJ to recyc...

  14. Municipal Treated Wastewater Irrigation: Microbiological Risk Evaluation

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    Antonio Lonigro

    2008-06-01

    Full Text Available Municipal wastewater for irrigation, though treated, can contain substances and pathogens toxic for humans and animals. Pathogens, although not harmful from an agronomical aspect, undoubtedly represent a major concern with regards to sanitary and hygienic profile. In fact, vegetable crops irrigated with treated wastewater exalt the risk of infection since these products can also be eaten raw, as well as transformed or cooked. Practically, the evaluation of the microbiological risk is important to verify if the microbial limits imposed by law for treated municipal wastewater for irrigation, are valid, thus justifying the treatments costs, or if they are too low and, therefore, they don’ t justify them. Different probabilistic models have been studied to assess the microbiological risk; among these, the Beta-Poisson model resulted the most reliable. Thus, the Dipartimento di Scienze delle Produzioni Vegetali of the University of Bari, which has been carrying out researches on irrigation with municipal filtered wastewater for several years, considered interesting to verify if the microbial limits imposed by the italian law n.185/03 are too severe, estimating the biological risk by the probabilistic Beta-Poisson model. Results of field trials on vegetable crops irrigated by municipal filtered wastewater, processed by the Beta-Poisson model, show that the probability to get infection and/or illness is extremely low, and that the actual italian microbial limits are excessively restrictive.

  15. Microbiology and evisceration efficiency of broiler carcasses slaughtered and held up to 8 hours postmortem prior to scalding and defeathering

    Science.gov (United States)

    The implementation of on farm slaughter could eliminate potential animal welfare issues associated with cooping, transport, dumping, and shackling live broilers. This research evaluated evisceration efficiency and the microbiological implications of delaying scalding and defeathering for up to 8 h a...

  16. Instrumental colour measurement as a tool for light veal carcasses online evaluation

    OpenAIRE

    Horcada Ibáñez, Alberto Luis; Molina, Antonio de; Valera Córdoba, María Mercedes; Beriain, María José; Juárez Dávila, Manuel María

    2013-01-01

    Eight-hundred and forty-five veal carcasses (535 males and 310 females) from Pirenaica breed and its crosses with Charolais and Limousin were used to compare carcass colour visual assessments 60min post-mortem with the values obtained using a spectrocolorimeter (CIE Lab colour system) on the pectoralis superficialis muscle. Carcass weights ranged between 240 and 360 kg. Visual colour was subjectively evaluated by two trained assessors. The best correlation between visual apprai...

  17. Development of a colour scale for colour evaluation of beef carcasses at 60 min post mortem

    NARCIS (Netherlands)

    Hulsegge, I.; Merkus, G.S.M.

    1999-01-01

    The pectoralis profundus muscles of 200 selected beef carcasses were measured to develop a standard colour scale for evaluating beef carcasses at the time of classification (approximately 60 min post mortem). Five lightness (L*) values were chosen ranging from 22 to 42 with intervals of five L* unit

  18. [Microbiologic evaluation of instant soup concentrates].

    Science.gov (United States)

    Wójcik-Stopczyńska, Barbara; Falkowski, Joachim; Jakubowska, Barbara

    2002-01-01

    The microbiological condition of instant soup powders purchased in retail network has been assessed. The study included 37 instant soups (8 types) manufactured by four Polish companies. The microbiological quality of a majority of soup powders fulfilled the requirements of the standard. No pathogenic bacteria (Salmonella, E. coli and Staphylococcus aureus) were detected, nor were there any spores of sulphitereducing anaerobic bacteria found. However, some samples of powders, mainly from one manufacturer, did have an excessive total number of bacteria (> 10(5) cfu/g) and a reduced (down to 0.01 g) level of coliform count. Aerobic bacteria occurring in powders were of vegetative and spore forms and exhibited the activity of amylo-, lipo- and proteolytic exoenzymes. The quantity of moulds did not exceed 100 cfu/g in a majority of samples. They were mainly represented by Penicillium sp., Aspergillus sp., Alternaria sp. and Cladosporium sp. PMID:12235671

  19. Evaluation of F1 calves sired by Brahman, Boran, and Tuli bulls for birth, growth, size, and carcass characteristics.

    Science.gov (United States)

    Herring, A D; Sanders, J O; Knutson, R E; Lunt, D K

    1996-05-01

    Birth (n = 308), weaning (n = 291), feedlot and carcass (n = 142), and yearling heifer traits (n = 139) were evaluated in F1 calves sired by Brahman (BR), Boran (BO), and Tuli (TU) bulls and born to multiparous Hereford and Angus cows. Calves sired by BR were heaviest (P Brahman crosses had larger (P Carcasses of BR crosses were heavier (P Brahman F1 heifers had larger (P carcass quality traits, but not for carcass yield traits, among these three breeds.

  20. The development and evaluation of reference materials for food microbiology.

    NARCIS (Netherlands)

    in 't Veld, P.H.

    1998-01-01

    Since 1986 the National Institute of Public Health and the Environment (RIVM) has worked on the development and evaluation of microbiological reference materials (RMs) with support from the European Communities Bureau of Reference (BCR), now called Standards Measurement and Testing (SM&T). The RMs a

  1. Microbiological evaluation of chicken feet intended for human consumption

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    Ana Paula Dutra Resem Brizio

    2013-12-01

    Full Text Available Chicken feet are products with great commercial importance for the eastern markets. Although Brazil is a large exporter of these products to those markets, little information is available on the sanitary quality of these products. The objective of this study was to evaluate the microbiological quality of frozen chicken feet for human consumption. This study was developed in a slaughterhouse under Federal Inspection, located in the state of Rio Grande do Sul. A total of 98 samples of frozen chicken feet were analyzed, between January and December 2011, for the detection of Salmonella spp., total count of mesophilic bacteria, total coliforms, fecal coliforms, Escherichia coli, Staphylococcus coagulase positive and Clostridium perfringens. About 99% of the results were within the microbiological standards established by the Chinese (world´s largest importer and Brazilian legislation for raw chicken meat. Thus, we conclude that the samples of frozen chicken feet showed satisfactory microbiological quality and no risk to consumer health.

  2. High pressure spray with water shows similar efficiency to trimming in controlling microorganisms on poultry carcasses.

    Science.gov (United States)

    Giombelli, Audecir; Hammerschmitt, Dandara; Cerutti, Marisete F; Chiarini, Eb; Landgraf, Mariza; Franco, Bernardete D G M; Destro, Maria T

    2015-10-01

    A study was conducted to evaluate a high pressure spray (HPS) with water as an alternative to trimming to remove gastrointestinal contamination on poultry carcasses and improve microbiological quality. The study was conducted under commercial conditions in 5 slaughter plants with one plant presenting approximately 5% of carcasses with visible gastrointestinal contamination (VGC), and the others showing approximately 12% of VGC. In all 5 plants, carcasses were sampled from the slaughter line and separated into 6 groups corresponding to 3 different treatments: A) carcasses with VGC before and after trimming; B) carcasses with VGC before and after HPS; and C) carcasses with no VGC before and after HPS. At the end of Trial A and prior to Trials B and C, an HPS equipment was installed before the end of the slaughter line. The HPS equipment was operated with 10 kgf/cm² of pressure and 1.5 L of potable water per carcass. Carcasses were analyzed using a rinsing procedure, and the following microbiological parameters were evaluated: the prevalence of Salmonella and Campylobacter, the abundance of Escherichia coli (EC), Enterobacteriaceae (EB), and the Total Viable Count (TVC). Salmonella was found in all plants at a prevalence ranging from 0.8% (plant 1) to 17.3% (plant 5), and the difference between plants was significant (P 0.05%) in any treatments. These results demonstrate that HPS with water is an alternative method for removing VGC and improving or maintaining the microbiological quality and safety of broiler carcasses. PMID:26286999

  3. Quality evaluation of beef carcasses produced under tropical conditions of México.

    Science.gov (United States)

    Zorrilla-Rios, J M; Lancaster, P A; Goad, C L; Horn, G W; Hilton, G G; Galindo, J G

    2013-01-01

    Twenty-three thousand four hundred eighty-four beef carcasses were classified according to the Mexican norm NMX-FF-078-SCFI-2002 at the No. 51 Federal Inspected Type abattoir located in the State of Tabasco, Mexico, owned by the Beef Cattle Union of Tabasco State. Tabasco State has a Tropical humid (Am) and subhumid (Aw) climate with rains in summer. The study took place between November 2009 and February 2010. The objective of this study was to evaluate independently the influence of each of the grading criteria used for classification on the final quality grade and determine areas for improvement to enhance the quality of Mexican beef carcasses. The beef carcass norm implementation followed a mechanistic approach of the 5 basic evaluation criteria applied in the sequential order: 1) maturity (age), 2) conformation (muscularity), 3) color of the meat, 4) fat color, and 5) distribution of the subcutaneous fat. The quality grades possible were Supreme, Select, Standard, Commercial, Out of Grade, and Veal. The proportion of carcasses classified as Select, Standard, Commercial, Out of Grade, and Veal were 13.4, 45.8, 27.4, 10.6, and 2.7%, respectively. No carcasses had a final quality grade of Supreme. Based on maturity, 79.2% of the carcasses met the specifications for Supreme; however, when the next criterion, conformation, was evaluated only 0.5% of the carcasses met the specifications for Supreme. When carcasses with a criterion grade of Supreme are not included in the analysis, the γ and κ statistics indicated that maturity and conformation have the greatest association and agreement, respectively, with final quality grade. When carcasses with a criterion grade of Supreme are included in the analysis, the κ statistic for the assessment of agreement between final quality grade and criterion indicated a descending order of conformation, subcutaneous fat distribution, maturity, meat color, and fat color. Thus, based on the degree of association and agreement

  4. Microbiological counting in lamb carcasses from an abattoir in São Paulo, Brazil Contagens microbiológicas em carcaças ovinas de um abatedouro de São Paulo, Brasil

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    Thaís Mioto Martineli

    2009-09-01

    Full Text Available The consumption of lamb meat in Brazil has increased in the last years but little information about the microbiological quality of this product is available. To evaluate the hygienic-sanitary conditions of lamb carcasses, the quantification of microorganism populations indicators (mesophiles and psychrotrophs; total and thermotolerant coliforms; Escherichia coli; moulds and yeasts and the pathogenic microorganisms indentification (Salmonella sp. and Listeria spp. were performed. A total of 60 lamb carcasses were sampled from one abattoir in São Paulo. Swab samples were collected from three points (forequarter, back and hindquarter on the muscle surface after carcasses final washing. Statistical analysis consisted of descriptive evaluation of the results whose counts were grouped by intervals of microorganism populations. Counts ranged from 1.0 x 10¹ to 8.0 x 10(4 colony-forming unit cm-2 (CFU cm-2 for mesophiles; 1.0 x 10(0 to 4.4 x 10(4CFU cm-2 for psychrotrophs; 32.0 most probable number/cm² (MPN cm-2 for total and thermotolerant coliforms and Escherichia coli. Salmonella sp. and Listeria spp. were not found in any of the carcasses. Most carcasses presented low counts for all microorganisms. Overall results may be explained by the small size of the industry where the study was taken. Results suggest that good microbiological quality lamb meat is possible to be obtained, but improvement in hygienic-sanitary conditions is still required.O consumo de carne ovina tem aumentado nos últimos anos, no Brasil. Entretanto, pouca informação sobre a qualidade desse produto está disponível. Com o intuito de avaliar as condições higiênico-sanitárias das carcaças ovinas, foram realizadas a quantificação das populações de microrganismos indicadores (mesófilos e psicrotróficos; coliformes totais e termotolerantes; Escherichia coli; bolores e leveduras e a identificação de microrganismos patogênicos (Salmonella sp. and Listeria spp.. Um

  5. A microbiological surrogate for evaluating treatment efficiency

    Energy Technology Data Exchange (ETDEWEB)

    Rice, E.W.; Fox, K.W.; Miltner, R.J. [Environmental Protection Agency, Cincinnati, OH (United States)] [and others

    1995-10-01

    In this study we report on the use of a microbial surrogate system which can be used to evaluate the efficiency of various unit processes used in drinking water treatment for the removal of microbial contaminants. The proposed procedure uses Gram-positive, mesophilic, aerobic spore-forming bacteria as the surrogate organisms. These bacteria do not pose a public health threat and are naturally occurring in most surface water supplies. The aerobic spore-formers are easy to culture and are present throughout the treatment train. This group of organisms consists primarily of species of the genus Bacillus. These organisms form endospores which are ellipsoidal to spherical in shape and measure on average approximately 0.5 X 1.0 X 1.5 micrometers, and are environmentally resistant. Like pathogenic Giardia cysts and Cryptosporidium oocysts endospores of aerobic bacilli may be found far into the treatment train.

  6. Evaluation of F1 calves sired by Brahman, Boran, and Tuli bulls for birth, growth, size, and carcass characteristics.

    Science.gov (United States)

    Herring, A D; Sanders, J O; Knutson, R E; Lunt, D K

    1996-05-01

    Birth (n = 308), weaning (n = 291), feedlot and carcass (n = 142), and yearling heifer traits (n = 139) were evaluated in F1 calves sired by Brahman (BR), Boran (BO), and Tuli (TU) bulls and born to multiparous Hereford and Angus cows. Calves sired by BR were heaviest (P Brahman crosses had larger (P Brahman F1 heifers had larger (P carcass quality traits, but not for carcass yield traits, among these three breeds.

  7. Performance and carcass quality of lambs evaluated in two feeding systems

    Directory of Open Access Journals (Sweden)

    Jaqueline Freitas Motta

    2016-03-01

    Full Text Available The objective of this study was to evaluate the productive performance and carcass quality of Corriedale crossbred lambs (n=28 in two feeding systems during finishing: a weaned and grazing on annual ryegrass (Lolium multiflorum Lam. in late season; b maintained with their mothers and grazing on May-deferred native pasture. The following measurements were made at the beginning of the experimental period and at intervals of 20 days: body weight, front height, rear height, body length, chest circumference, and body condition. Additionally, body structure was evaluated by the frame size index [FSI = (front height + rear height + body length + body weight/4], and compactness as body weight/body length. Eight animals were slaughtered at the end of the experimental period, four per treatment, for the evaluation of carcass traits (body and regional components, cooking loss, and carcass yields (commercial, slaughter, and farm. The data were submitted to analysis of variance and means were compared by the F test or by the nonparametric Wilcoxon-Mann-Whitney test (P0.05. However, animals maintained with their mothers on deferred native pasture showed better productive performance characterized by higher total gains in weight, body condition, chest circumference, and compactness. Postslaughter evaluation showed significantly higher (P<0.05 hot and cold carcass weight and better commercial and slaughter carcass yields, in addition to longer carcasses with greater depth and leg proportion in lambs kept with their mothers on native pasture compared to those kept on annual ryegrass, which only exhibited a greater percentage of gastrointestinal tract.

  8. Evaluation of DNA polymorphisms involving growth hormone relative to growth and carcass characteristics in Brahman steers.

    Science.gov (United States)

    Beauchemin, V R; Thomas, M G; Franke, D E; Silver, G A

    2006-01-01

    Associations of DNA polymorphisms in growth hormone (GH) relative to growth and carcass characteristics in growing Brahman steers (N = 324 from 68 sires) were evaluated. Polymorphisms were an Msp-I RFLP and a leucine/valine SNP in the GH gene as well as a Hinf-I RFLP and a histidine/arginine SNP in transcriptional regulators of the GH gene, Pit-1 and Prop-1. Genotypic frequencies of the GH SNP, Pit-1 RFLP, and Prop-1 SNP were greater than 88% for one of the bi-allelic homozygous genotypes. Genotypic frequencies for the GH Msp-I RFLP genotypes were more evenly distributed with frequencies of 0.43, 0.42, and 0.15 for the genotypes of +/+, +/-, and -/-, respectively. Mixed model analyses of growth and carcass traits with genotype and contemporary group serving as fixed effects and sire fitted as a random effect suggested that sire was a significant source of variation (P carcass yield, and marbling score. However, measures of growth and carcass traits were similar across GH Msp-I genotypes as steers were slaughtered when fat thickness was estimated to be approximately 1.0 cm. These polymorphisms within the GH gene and/or its transcriptional regulators do not appear to be informative predictors of growth and carcass characteristics in Brahman steers. This is partly due to the high level of homozygosity of genotypes. These findings do not eliminate the potential importance of these polymorphisms as predictors of growth and carcass traits in Bos taurus or Bos taurus x Bos indicus composite cattle.

  9. Evaluation of DNA polymorphisms involving growth hormone relative to growth and carcass characteristics in Brahman steers.

    Science.gov (United States)

    Beauchemin, V R; Thomas, M G; Franke, D E; Silver, G A

    2006-07-31

    Associations of DNA polymorphisms in growth hormone (GH) relative to growth and carcass characteristics in growing Brahman steers (N = 324 from 68 sires) were evaluated. Polymorphisms were an Msp-I RFLP and a leucine/valine SNP in the GH gene as well as a Hinf-I RFLP and a histidine/arginine SNP in transcriptional regulators of the GH gene, Pit-1 and Prop-1. Genotypic frequencies of the GH SNP, Pit-1 RFLP, and Prop-1 SNP were greater than 88% for one of the bi-allelic homozygous genotypes. Genotypic frequencies for the GH Msp-I RFLP genotypes were more evenly distributed with frequencies of 0.43, 0.42, and 0.15 for the genotypes of +/+, +/-, and -/-, respectively. Mixed model analyses of growth and carcass traits with genotype and contemporary group serving as fixed effects and sire fitted as a random effect suggested that sire was a significant source of variation (P carcass yield, and marbling score. However, measures of growth and carcass traits were similar across GH Msp-I genotypes as steers were slaughtered when fat thickness was estimated to be approximately 1.0 cm. These polymorphisms within the GH gene and/or its transcriptional regulators do not appear to be informative predictors of growth and carcass characteristics in Brahman steers. This is partly due to the high level of homozygosity of genotypes. These findings do not eliminate the potential importance of these polymorphisms as predictors of growth and carcass traits in Bos taurus or Bos taurus x Bos indicus composite cattle.

  10. Genetic evaluation of beef carcass data using different endpoint adjustments.

    Science.gov (United States)

    Rumph, J M; Shafer, W R; Crews, D H; Enns, R M; Lipsey, R J; Quaas, R L; Pollak, E J

    2007-05-01

    Carcass data from 6,795 Simmental-sired animals born from 1992 to 2001 were used to determine whether adjustment to a constant age, back-fat, HCW, or marbling score would result in differences in heritability of the carcass traits and, correspondingly, if EPD calculated using those variance components and adjustments would result in sire reranking. The endpoints were age (EPA), backfat (EPF), HCW (EPC), or marbling (EPM). The traits analyzed were 12th-rib backfat (FAT), HCW, marbling (MRB), LM area (LMA), and percentage retail cuts (PRC). The data were analyzed using an animal model, where contemporary group was included as a fixed effect and was composed of slaughter date, sex, and herd. Random effects included in the model were direct genetic and residual. Estimates of heritability ranged from 0.12 to 0.14, 0.32 to 0.34, and 0.26 to 0.27 for FAT, HCW, and LMA, respectively, for the corresponding endpoints. Heritability for MRB was estimated to be 0.27 at all endpoints. For PRC, estimates of heritability were more variable, with estimates of 0.23 +/- 0.05, 0.32 +/- 0.05, 0.21 +/- 0.05, and 0.20 +/- 0.04 for EPA, EPF, EPC, and EPM, respectively. However, because the EPF and EPC adjustments adjust for a component trait of PRC (FAT and HCW, respectively), they may be altering the trait to one different from PRC. Spearman rank correlations between EPD within a trait using EPA compared with the other endpoints were >0.90 (P < 0.01) for FAT, HCW, MRB, and LMA. For PRC, Spearman rank correlations with EPA EPD were 0.73 (P < 0.01), 0.93 (P < 0.01), and 0.95 (P < 0.01) for EPF, EPC, and EPM, respectively. For most traits and endpoints, there was little reranking among sires when alternative endpoints were used. However, adjusting PRC to EPF appears to result in a greater heritability and substantial re-ranking of sires, potentially due to the adjustment changing the trait to one other than PRC. PMID:17224467

  11. Evaluation of carcass traits and meat characteristics of Guzerat-crossbred bulls.

    Science.gov (United States)

    Diniz, Frederico B; Villela, Severino D J; Mourthé, Mário H F; Paulino, Pedro V R; Boari, Cleube A; Ribeiro, Julimar S; Barroso, Jorge A; Pires, Aldrin V; Martins, Paulo G M A

    2016-02-01

    Our objective was to evaluate carcass and meat characteristics of Guzerat-crossbred bulls finished in feedlot. Carcasses from 18 bulls, randomly selected from a larger group of 36 bulls, consisting of F1 Guzerat×Holstein ("Guzholstein"); F1 Guzerat×Nellore ("Guzonell"); and 1/2 Simmental+1/4 Guzerat+1/4 Nellore (Three-Cross; n=6 each group) were used. Cold carcass weight was greater (P=0.01) for Three-Cross compared with "Guzonell" and "Guzholstein". Three-Cross carcasses had greater (P0.05) among groups, but depth was greater (P<0.01) for Three-Cross compared with other groups. "Guzholtein" had lesser (P=0.05) shear force compared with "Guzonell", with Three-Cross being intermediate. We conclude that "Guzholstein" is an adequate option for producers willing to finish this kind of genetic group, as it is comparable or better than Bos indicus crosses and B. indicus×Bos taurus bulls. PMID:26546913

  12. Evaluation of antimicrobial activities of sequential spray applications of decontamination treatments on chicken carcasses.

    Science.gov (United States)

    Benli, Hakan; Sanchez-Plata, Marcos X; Ilhak, Osman Irfan; Núñez De González, Maryuri T; Keeton, Jimmy T

    2015-03-01

    The objective of this study was to evaluate the effects of sequential applications of ɛ-polylysine (EPL) or lauramide arginine ethyl ester (LAE) sprays followed by an acidic calcium sulfate (ACS) spray on inoculated chicken carcasses to reduce Salmonella (Salmonella enterica serovars including Salmonella typhimurium and Salmonella enteritidis) contamination during 6 days of storage (4.4°C). Secondly, reductions of the resident microflora were studied on uninoculated chicken carcasses following the sequential application of the treatments, chilling and 10 days of storage at 4.4°C. The treatment of Salmonella inoculated carcasses with 300 mg/L EPL followed by 30% ACS (EPL300-ACS30) sprays reduced Salmonella counts initially by 1.5 log cfu/mL and then by 1.2 log cfu/mL (pEPL300-ACS30 and LAE200-ACS30 both reduced Escherichia coli counts significantly by 2.6 and 2.9 log cfu/mL, respectively. EPL300-ACS30 and LAE200-ASC30 were effective in lowering psychrotroph counts by 1 log cfu/mL on day 10 when compared to the control and distilled water treatments. This study demonstrated that EPL300-ACS30 and LAE200-ACS30 were effective in reducing Salmonella on inoculated chicken carcasses both after treatment and during the storage at 4.4°C for up to 6 days. In addition, reductions in psychrotroph counts indicated that these treatments might have the potential to increase the shelf-life of poultry carcasses. PMID:25656180

  13. An Evaluation of Computer-Based Instruction in Microbiology

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    Susan M. Merkel

    2009-12-01

    Full Text Available There has been a tremendous increase in the availability of computer-based instructional (CBI materials. Some studies have shown an improvement in learning when CBI is used. However, many researchers believe the current studies are inadequate. While CBI software should be thoroughly tested by developers, as educators, we should be concerned about whether or not the CBI materials we use are improving learning in our classrooms with our students. We present an evaluation of a computer-based hypermedia tutorial that was delivered over our General Microbiology website. We found that CBI was at least as effective as text-based material. However, of all students who used CBI, only those who explored most of the site benefited from using the site. Tracking each student's use of the CBI was critical for understanding who was learning and why.

  14. An Evaluation of Computer-Based Instruction in Microbiology

    Directory of Open Access Journals (Sweden)

    Jerry S. Leventhal

    2000-12-01

    Full Text Available There has been a tremendous increase in the availability of computer-based instructional (CBI materials. Some studies have shown an improvement in learning when CBI is used. However, many researchers believe the current studies are inadequate. While CBI software should be thoroughly tested by developers, as educators, we should be concerned about whether or not the CBI materials we use are improving learning in our classrooms with our students. We present an evaluation of a computer-based hypermedia tutorial that was delivered over our General Microbiology website. We found that CBI was at least as effective as text-based material. However, of all students who used CBI, only those who explored most of the site benefited from using the site. Tracking each student's use of the CBI was critical for understanding who was learning and why.

  15. Evaluation of an online program to teach microbiology to internal medicine residents.

    Science.gov (United States)

    Guarner, Jeannette; Burd, Eileen M; Kraft, Colleen S; Armstrong, Wendy S; Lenorr, Kenya; Spicer, Jennifer O; Martin, Donna; del Rio, Carlos

    2015-01-01

    Microbiology rounds are an integral part of infectious disease consultation service. During microbiology rounds, we highlight microbiology principles using vignettes. We created case-based, interactive, microbiology online modules similar to the vignettes presented during microbiology rounds. Since internal medicine residents rotating on our infectious disease elective have limited time to participate in rounds and learn microbiology, our objective was to evaluate the use of the microbiology online modules by internal medicine residents. We asked residents to complete 10 of 25 online modules during their infectious disease elective. We evaluated which modules they chose and the change in their knowledge level. Forty-six internal medicine residents completed assessments given before and after accessing the modules with an average of 11/20 (range, 6 to 19) and 16/20 (range, 9 to 20) correct questions, respectively (average improvement, 5 questions; P = 0.0001). The modules accessed by more than 30 residents included those related to Clostridium difficile, anaerobes, Candida spp., Streptococcus pneumoniae, influenza, Mycobacterium tuberculosis, and Neisseria meningitidis. We demonstrated improved microbiology knowledge after completion of the online modules. This improvement may not be solely attributed to completing the online modules, as fellows and faculty may have provided additional microbiology education during the rotation.

  16. Preliminary Evaluation of Slaughter Value and Carcass Composition of Indigenous Sheep and Goats from Traditional Production System in Tanzania

    OpenAIRE

    Shija, Dismas S.; Mtenga, Louis A.; Kimambo, Abiliza E.; Laswai, Germana H.; Mushi, Daniel E.; Mgheni, Dynes M.; Mwilawa, Angello J.; Shirima, Eligy J. M.; Safari, John G.

    2013-01-01

    The aim of the pilot study was to evaluate slaughter characteristics and carcass composition of indigenous long fat tailed sheep and Small East African goats purchased from the auction markets slaughtered at 1.5 to 2 yrs of age and 20 kg to 25 kg live weight. The animals were slaughtered according to halal standard procedures. The left half carcasses were jointed into eight wholesale joints, and dissected into muscles, fat and bone, which were weighed separately. Sheep had greater (p

  17. Evaluation of Microbiological Criteria of Raw Milk in Albania

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    GENTIANA BARDHI

    2014-06-01

    Full Text Available This paper gives the results of the Albanian monitoring plan for the raw milk in 121 domestic subjects: producing farms, collecting points and processing plants. The main objective for this monitoring was the evaluation of milk microbiological criteria. This monitoring was carried out during the year 2013 separated in two phases: 1-31 May and 1-31 October. Milk sampling was made by Regional Directorates Inspectors of National Food Authority in conformity to S SH ISO 707:1999 “Sampling method of milk and milk by-products. The microbial evaluation was carried out based on the norms laid down in Decision of Council of Ministers Nr.1132 date 05.08.2008: “Approving norms for collecting raw milk”. 415 samples were analyzed in total for the total content of micro flora as well as somatic cells. The TMC test was determined according to ISO 4833:2003 and SCC was determined according to White-Side-Test (WST, California Mastitis Test (CMT and ISO 13366-1:1997. The results taken for the two phases of the monitoring plan has shown unsatisfactory results. This indicates that is necessary to improve the existing situation in milk producing farms, collecting points and processing plants as well.

  18. Evaluation of Microbiological and Chemical Contaminants in Poultry Farms

    Directory of Open Access Journals (Sweden)

    Justyna Skóra

    2016-02-01

    Full Text Available The aim of the study was to evaluate the microbiological and chemical contamination in settled dust at poultry farms. The scope of research included evaluating the contributions of the various granulometric fractions in settled dust samples, assessing microbial contamination using culture methods, concentrations of secondary metabolites in dust and their cytotoxicity against hepatocyte chicken cells by means of MTT (3-(4,5-dimethylthiazolyl-2-2,5-diphenyltetrazolium bromide tests. In addition, we also evaluated the concentration of selected volatile odorous compounds (VOCs using gas chromatographic and spectrophotometric methods and airborne dust concentration in the air with DustTrak™ DRX Aerosol Monitor. Studies were carried out on chicken broilers and laying hens at 13 poultry farms, with numbers of birds ranging from 8000 to 42,000. The airborne total dust concentration at poultry farms averaged 1.44 mg/m3 with a high percentage of the PM10 fraction (particulate matter with a diameter less than 10 μm. Microorganism concentrations in the settled dust were: 3.2 × 109 cfu/g for bacteria and 1.2 × 106 cfu/g for fungi. Potential pathogens (Enterococcus spp., Escherichia coli, Salmonella spp., Aspergillus fumigatus, Paecilomyces variotii were also found. Secondary metabolites included aurofusarin, deoxynivalenol, 15-hydroxyculmorin zearalenone, zearalenone-sulfate, infectopyron, and neochinulin A. However, the dust samples showed weak cytotoxicity towards chicken hepatocyte cells, which ranged between 9.2% and 29.7%. Among volatile odorous compounds ammonia, acrolein, methyloamine, acetic acid, acetoaldehyde and formaldehyde were detected in the air. In conclusion, settled dust can be a carrier of microorganisms, odours and secondary metabolites in poultry farms, which can be harmful to workers’ health.

  19. Evaluation of Microbiological and Chemical Contaminants in Poultry Farms.

    Science.gov (United States)

    Skóra, Justyna; Matusiak, Katarzyna; Wojewódzki, Piotr; Nowak, Adriana; Sulyok, Michael; Ligocka, Anna; Okrasa, Małgorzata; Hermann, Janusz; Gutarowska, Beata

    2016-02-01

    The aim of the study was to evaluate the microbiological and chemical contamination in settled dust at poultry farms. The scope of research included evaluating the contributions of the various granulometric fractions in settled dust samples, assessing microbial contamination using culture methods, concentrations of secondary metabolites in dust and their cytotoxicity against hepatocyte chicken cells by means of MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) tests. In addition, we also evaluated the concentration of selected volatile odorous compounds (VOCs) using gas chromatographic and spectrophotometric methods and airborne dust concentration in the air with DustTrak™ DRX Aerosol Monitor. Studies were carried out on chicken broilers and laying hens at 13 poultry farms, with numbers of birds ranging from 8000 to 42,000. The airborne total dust concentration at poultry farms averaged 1.44 mg/m³ with a high percentage of the PM10 fraction (particulate matter with a diameter less than 10 μm). Microorganism concentrations in the settled dust were: 3.2 × 10⁸ cfu/g for bacteria and 1.2 × 10⁶ cfu/g for fungi. Potential pathogens (Enterococcus spp., Escherichia coli, Salmonella spp., Aspergillus fumigatus, Paecilomyces variotii) were also found. Secondary metabolites included aurofusarin, deoxynivalenol, 15-hydroxyculmorin zearalenone, zearalenone-sulfate, infectopyron, and neochinulin A. However, the dust samples showed weak cytotoxicity towards chicken hepatocyte cells, which ranged between 9.2% and 29.7%. Among volatile odorous compounds ammonia, acrolein, methyloamine, acetic acid, acetoaldehyde and formaldehyde were detected in the air. In conclusion, settled dust can be a carrier of microorganisms, odours and secondary metabolites in poultry farms, which can be harmful to workers' health.

  20. Chemical, Nutritional and Microbiological Evaluation of Some Egyptian Soft Cheeses

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    *Ghada, Z. A. A., 2*Alia, M. H., 3**Soha, Al-S., 4*Magdy, N. A., and 5*Mohammed, F. S

    2004-12-01

    Full Text Available Milk and dairy products is considered the most complete foodstuff that provide human either infants or adults with most of their vital needs. Milk and cheese have high nutritive value due to its high content of protein, fat, minerals especially calcium (Ca2+ & phosphorous, and vitamins. Two hundred samples produced and sold in Egypt during 2001-2003 were collected from allover the country. The cheese samples were subjected to microbiological and chemical analysis. Samples were microbiologically tested for total aerobic bacterial count (TABC, Colifrm, Escherichia coli (E. coli, Staphylococcus aureus, mould and yeast, salmonella and shigella, and listeria species. Protein, fat, carbohydrates, moisture, ash, lactose, Calcium (Ca, phosphorous (P and Ca/P were evaluated. The analysis showed that total aerobic bacterial count did not exceed 1.4X105±1.7X105 cells/gm, which is close to what allowed by the Standard Egyptian Guidelines (2001 and 47.5 % of the tested cheese are free from coliform bacteria and Escherichia coli. Ninety-eight and half percent, 97 %, 97 % and 91.5 % of the tested cheese (kareish, feta, thalaga, double cream respectively, either made in plant or home or farmers' cheese sample have zero Staphylococcus aureus count or mould and yeast; or salmonella and shigella, or listeria species respectively, i. e. free from them. Double cream cheese has the lowest protein content (7.79±0.78 gm% while kareish cheese has the highest protein content (19.99±1.32 gm%, but for fat content the opposite is true, double cream cheese have the highest fat content (24.56±1.78 gm% while kareish cheese have the lowest fat content (3.87±0.97 gm %. Feta cheese has high ash content while kareish cheese has the highest moisture content with the lowest ash content (68.97±1.86 & 1.81±0.47 gm% respectively. Lactose content varies widely from 1.50±0.26 (double cream cheese to 3.25±0.50 (feta cheese. Kareish cheese has higher content of calcium and

  1. Evaluation of carcass characteristics and meat chemical composition of Bos indicus and Bos indicus x Bos taurus crossbred steers finished in pasture systems

    OpenAIRE

    Moreira Fernanda Barros; Souza Nilson Evelázio de; Matsushita Makoto; Prado Ivanor Nunes do; Nascimento Willian Gonçalves do

    2003-01-01

    The objective of this study was to evaluate the carcass characteristics (carcass weight, carcass yield, fat thickness, loin area, marbling and colour) and chemical composition of the Longissimus dorsi muscle (moisture, ash, crude protein, fat and cholesterol) of cuts with or without fat thickness, of Bos indicus and Bos indicus x Bos taurus crossbred steers finished in millet (Pennisetum americanum L.) or star grass (Cynodon plectostachyus Pilger) pasture systems, with mineral or mineral prot...

  2. Evaluation of performance characteristics in a diallel among Simmental, Limousin, Polled Hereford and Brahman beef cattle. II. Carcass traits.

    Science.gov (United States)

    Comerford, J W; Benyshek, L L; Bertrand, J K; Johnson, M H

    1988-02-01

    Evaluations of steer and heifer progeny from a diallel mating design of Simmental, Limousin, Polled Hereford and Brahman beef cattle over 5 yr are presented. Traits evaluated included final weight, hot carcass weight, ribeye area, 12th rib fat thickness, marbling score, yield grade, dressing percentage and percentage of kidney, pelvic and heart fat. Progeny of Simmental sires were heavier at slaughter than those with Brahman sires (P less than .05), but no differences were found for carcass weight. Dressing percentage was higher for Limousin crosses compared with progeny of other sire breeds (P less than .05). Similar results were found for dam breeds, except that progeny of Limousin dams had heavier carcasses with a higher dressing percentage (P less than .05) than Brahman crosses. Crosses of Limousin and Simmental had larger ribeye areas (P less than .05) compared with calves of the other breeds. Progeny of Polled Hereford dams had higher marbling scores and were fatter than progeny of dams of other breeds (P less than .05). Heterosis estimates were significant for all Brahman crosses for final weight, carcass weight and ribeye area, but these contrasts were negligible for other traits. Estimates of general combining ability were positive and significant for Simmental for final weight, carcass weight, ribeye area and marbling score and were significant and negative for Limousin for final weight, fat thickness and yield grade. Maternal values were generally small. PMID:3372376

  3. Evaluation of performance characteristics in a diallel among Simmental, Limousin, Polled Hereford and Brahman beef cattle. II. Carcass traits.

    Science.gov (United States)

    Comerford, J W; Benyshek, L L; Bertrand, J K; Johnson, M H

    1988-02-01

    Evaluations of steer and heifer progeny from a diallel mating design of Simmental, Limousin, Polled Hereford and Brahman beef cattle over 5 yr are presented. Traits evaluated included final weight, hot carcass weight, ribeye area, 12th rib fat thickness, marbling score, yield grade, dressing percentage and percentage of kidney, pelvic and heart fat. Progeny of Simmental sires were heavier at slaughter than those with Brahman sires (P less than .05), but no differences were found for carcass weight. Dressing percentage was higher for Limousin crosses compared with progeny of other sire breeds (P less than .05). Similar results were found for dam breeds, except that progeny of Limousin dams had heavier carcasses with a higher dressing percentage (P less than .05) than Brahman crosses. Crosses of Limousin and Simmental had larger ribeye areas (P less than .05) compared with calves of the other breeds. Progeny of Polled Hereford dams had higher marbling scores and were fatter than progeny of dams of other breeds (P less than .05). Heterosis estimates were significant for all Brahman crosses for final weight, carcass weight and ribeye area, but these contrasts were negligible for other traits. Estimates of general combining ability were positive and significant for Simmental for final weight, carcass weight, ribeye area and marbling score and were significant and negative for Limousin for final weight, fat thickness and yield grade. Maternal values were generally small.

  4. Microbiological characterisation of soils : Evaluation of some critical steps in data collection and experimental design

    OpenAIRE

    Wallenius,Kaisa

    2011-01-01

    The study of soil microbiota and their activities is central to the understanding of many ecosystem processes such as decomposition and nutrient cycling. The collection of microbiological data from soils generally involves several sequential steps of sampling, pretreatment and laboratory measurements. The reliability of results is dependent on reliable methods in every step. The aim of this thesis was to critically evaluate some central methods and procedures used in soil microbiological...

  5. Evaluation of Physicochemical and Microbiological Parameters of Smoked Sausages

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    Melinda Nagy

    2015-11-01

    Full Text Available Meat and meat products continue to supply nutrients and play a vital role in human life because of their high biological value protein, iron, zinc, selenium and vitamin B12 contents, being a crucial component of a well balanced diet. The objective of this paper was to analyse the microbiological and physicochemical characteristics of smoked sausage obtain by a modern recipe. The meat  material was obtained from local butchery (Cluj-Napoca, Romania. The physicochemical analyses highlighted the moisture (Drying-oven at 105 ºC, protein (Kjeldahl method and fat (Soxhlet method content, as well as nitrite (Griess method and sodium chloride concentrations (Mohr method of the final products. Microbiological and physicochemical analysis of the examined samples found no deviations from legal norms imposed for smoked sausage.  All of the quality parameters comply with the limits stipulated by STAS. 

  6. Evaluation of Physicochemical and Microbiological Parameters of Smoked Sausages

    OpenAIRE

    Melinda Nagy; Sonia A. Socaci; Maria Tofană; Crina Mureșan; Ana Viorica Pop (Cuceu); Carmen Pop

    2015-01-01

    Meat and meat products continue to supply nutrients and play a vital role in human life because of their high biological value protein, iron, zinc, selenium and vitamin B12 contents, being a crucial component of a well balanced diet. The objective of this paper was to analyse the microbiological and physicochemical characteristics of smoked sausage obtain by a modern recipe. The meat  material was obtained from local butchery (Cluj-Napoca, Romania). The physicochemical analyses highlighted th...

  7. Commercial babassu mesocarp: microbiological evaluation and analysis of label information

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    Laisa Lis Fontinele Sá

    2015-10-01

    Full Text Available The babassu mesocarp is easily found in supermarkets and other commercial establishments in Brazil. Despite its widespread use in both pharmaceutical and food industries, the literature has neither scientific studies about microbial contamination for these products nor about legal information expressed on label. The aim of this study was to evaluate the level of microbiological contamination in babassu mesocarp sold in commercial establishments in Teresina-PI/Brazil besides the conformity of label information according to the rules of Brazilian Sanitary Surveillance Agency (ANVISA. Ten samples of babassu mesocarp powder sold in the region were selected for study. Determination of heterotrophic microorganisms was carried out using the seeding technique of Plate Count Agar (CFU g-1. It was used Sabouraud Dextrose Agar medium for cultivation of fungi. For the analysis of label information, the resolutions (RDC, 259 of September 20, 2002, and 360 of December 23, 2003, beyond the law 10,674 of May 16, 2003 were used. The results of levels of contamination for heterotrophic bacteria and fungi showed high contamination for all samples. Most of the label samples were according to the rules. Therefore, the results suggest a more comprehensive monitoring of these microorganisms besides the development of more effective methods for decontamination of these products sold in Brazil.Keywords: Babassu. Label. Contamination. Food. Pharmacy. RESUMO O mesocarpo de babaçu é encontrado facilmente em supermercados e em outros estabelecimentos comercias e apesar de sua ampla utilização, tanto na indústria farmacêutica e de alimentos, na literatura não há trabalhos científicos que avaliem sua contaminação microbiológica ou informações legais necessárias para rótulos. O objetivo do estudo foi avaliar o nível de contaminação microbiológica do mesocarpo de babaçu, vendidos no comércio de Teresina-PI, bem como verificar a conformidade das informa

  8. Evaluation of activities aimed at preventing microbiological risks in dental practice

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    Jolanta Szymańska

    2013-02-01

    Full Text Available Background: Microbiological contamination of water in dental unit waterlines (DUWL creates a risk of cross-infections, and is a source of biological risk factors in the work environment of a dentist. The aim of the study was to evaluate dentists' knowledge on DUWL microbiological contamination and the scope of activities/procedures they undertake to monitor it. Material and Methods: The questionnaire survey was conducted in 2010 among 107 Polish dentists using dental units in everyday clinical practice. Results: It has been found that in their daily practice, dentists do not follow procedures leading to reduction or elimination of microbiological contamination of dental unit reservoir water. They are not aware of microbiological contamination of DUWL that supply working handpieces with water. They are unaware of the principles of dealing with dental water and water supply systems or the health risk posed by microbiological contamination of unit water for a dental team and patients. Conclusions: It is necessary to provide dentists with information on microbiological contamination of water in dental units, on the correct procedures of handling water and waterlines that supply working handpieces with water. Med Pr 2013;64(1:11–17

  9. MICROBIOLOGICAL EVALUATION OF ANTIBIOTIC RESIDUES IN MEAT, MILK AND EGGS

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    Abdul Jabbar

    2013-04-01

    Full Text Available Microbiological tests are widely used to detect antibiotic residues in the meat, milk and eggs for better care of the quality and health safety. In the present study microbiological inhibition test i.e. Swab Test on Animal Food (STAF was developed indigenously for screening of animal foods for presence of antibiotic residues. In this test local isolated culture of Bacillus subtilis was used as a test microorganism due to its high sensitivity to detect a wide range of antibiotics commonly used in animal disorders. The concentration of spore suspension of Bacillus subtilis JS2004 used in the formation of STAF plate was optimized at 2x107 spores/ ml. At this concentration, inhibition zone around Neomycin control disc was 10-16 mm. Nutrient agar was used as a medium in spore suspension and 0.4% dextrose was added as a constituent of medium. Zones of inhibition around swab samples and Neomycin control disc were observed and the diameter was measured. All swab samples showing a minimum of 2 mm wide inhibition zone around them were considered as positive for presence of antibiotic residues. The swab samples showing no zone of inhibition or a zone measuring less than 2 mm were considered as negative. Results of application of STAF test was on animal food samples revealed the high incidence of antibiotic residues.

  10. Genetic evaluation of carcass traits in Simmental-sired cattle at different slaughter end points.

    Science.gov (United States)

    Shanks, B C; Tess, M W; Kress, D D; Cunningham, B E

    2001-03-01

    Our objectives were to estimate genetic parameters for carcass traits and evaluate the influence of slaughter end point on estimated breeding values (BV). Data provided by the American Simmental Association were divided into three sets: 1) 9,604 records of hot carcass weight (CW) and percentage retail cuts (PRC), 2) 6,429 records of CW, PRC, and marbling score (MS), and 3) 1,780 records of CW, PRC, MS, fat thickness (FT), and longissimus muscle area (LMA). Weaning weights (WW) from animals with carcass data and from their weaning contemporaries were used. Data were analyzed with a multiple-trait animal model and REML procedures to estimate genetic parameters and BV on an age-, CW-, MS-, or FT-constant basis. The model for carcass traits included fixed contemporary group and covariates for breed, heterozygosity, and slaughter end point and random additive direct genetic and residual effects. Weaning weight was preadjusted for founder effects, direct and maternal heterosis, age of dam, and age of calf. The model for WW included fixed contemporary group and random additive direct genetic, maternal genetic, maternal permanent environment, and residual effects. Heritabilities from data set 1 were 0.34 for CW and 0.25 for PRC on an age-constant basis and 0.25 for PRC on a CW end point. Heritabilities for data set 2 were 0.35, 0.24, and 0.36 for CW, PRC, and MS, respectively, on an age-constant basis. Data set 2 heritabilities were 0.25 for PRC and 0.34 for MS on a CW-constant basis and 0.33 for CW and 0.25 for PRC at a constant MS end point. Heritabilities on an age-constant basis for data set 3 were as follows: CW, 0.32; PRC, 0.09; MS, 0.12; FT, 0.10; and LMA, 0.26. Heritability estimates for data set 3 on a CW-, MS-, and FT-constant basis were similar to those on an age-constant basis. Heritabilities were 0.12 for PRC, 0.12 for MS, 0.14 for FT, and 0.22 for LMA on a CW-constant basis; 0.30 for CW, 0.09 for PRC, 0.10 for FT, and 0.28 for LMA at a constant MS end point

  11. Hot water surface pasteurisation of lamb carcasses: microbial effects and cost-benefit considerations.

    Science.gov (United States)

    Hauge, Sigrun J; Wahlgren, Magnus; Røtterud, Ole-Johan; Nesbakken, Truls

    2011-03-15

    Although hot water pasteurisation of carcasses is accepted as a general intervention in USA, this is not the case in Europe. The aims of this study were (i) to evaluate the microbiological effects of hot water pasteurisation of lamb carcasses, both after slaughtering and dressing and following subsequent chilling and storage; (ii) to discuss hot water pasteurisation from a public health and cost-benefit perspective; (iii) to discuss the benefits of hot water pasteurisation compared with use of separate meat processing streams for high-risk carcasses; (iv) to evaluate the use of recycled hot water in a hygienic context and in relation to EU regulations; and (v) to consider the technological and sensory aspects of hot water pasteurisation of lamb carcasses. Samples were collected from 420 naturally contaminated lamb carcasses, with 50% of the carcasses (n=210) subject to hot water pasteurisation at 82 °C for 8s immediately after slaughter. Surface swab samples from 4500 cm² areas on carcasses were collected at slaughter, after chilling for 24 h, and after chilling for five days. The microbial analyses included Escherichia coli, Enterobacteriaceae, Bacillus cereus, Clostridium perfringens and aerobic plate count (APC). A resuscitation step using Tryptone Soya Agar was included in the microbiological analyses. Hot water pasteurisation significantly reduced the levels of E. coli, Enterobacteriaceae, B. cereus and APC (all Pgaps regarding hot water pasteurisation and indicate that replacing the expensive system of separate processing of high-risk carcasses with hot water surface pasteurisation should be considered as a serious option. PMID:21356564

  12. Evaluation of methods for removing central nervous system tissue contamination from the surface of beef carcasses after splitting.

    Science.gov (United States)

    Takada, Naoko; Horiuchi, Motohiro; Sata, Tetsutaro; Sawada, Yasushi

    2008-11-01

    Since high levels of prions, the causative agent of bovine spongiform encephalopathy (BSE), accumulate in the brain and spinal cord, contamination of beef carcasses with central nervous system tissue (CNST) may occur at post-slaughter process. In this study, we investigated CNST contamination on the surface of beef carcasses using glial fibrillary acidic protein as a marker after splitting and evaluated the effects of washing procedures on contamination removal. High levels of CNST contamination was detected immediately after splitting, especially in the area close to the spinal column. This suggests that spinal cord fragments are attached to carcasses at the time of splitting even though the spinal cords have been removed by vacuum before splitting. Steam cleaning or manually washing with normal pressure water around the spinal column, performed prior to washing with high-pressure water, was found to be effective for reducing the level of CNST contamination. Furthermore, manually washing with high-pressure water could reduce CNST contamination to almost negligible levels. These results are useful for preparation of appropriate sanitation standard operating procedures to reduce the risk of CNST contamination of carcasses for prevention of exposure to BSE prion via the food chain. PMID:19057142

  13. Evaluation of the Operator Protection Factors Offered by Positive Pressure Air Suits against Airborne Microbiological Challenge

    Directory of Open Access Journals (Sweden)

    Jackie A. Steward

    2012-08-01

    Full Text Available Laboratories throughout the world that perform work with Risk Group 4 Pathogens generally adopt one of two approaches within BSL-4 environments: either the use of positive pressure air-fed suits or using Class III microbiological safety cabinets and isolators for animal work. Within the UK at present, all laboratories working with Risk Group 4 agents adopt the use of Class III microbiological safety cabinet lines and isolators. Operator protection factors for the use of microbiological safety cabinets and isolators are available however; there is limited published data on the operator protection factors afforded by the use of positive pressure suits. This study evaluated the operator protection factors provided by positive pressure air suits against a realistic airborne microbiological challenge. The suits were tested, both intact and with their integrity compromised, on an animated mannequin within a stainless steel exposure chamber. The suits gave operator protection in all tests with an intact suit and with a cut in the leg. When compromised by a cut in the glove, a very small ingress of the challenge was seen as far as the wrist. This is likely to be due to the low airflow in the gloves of the suit. In all cases no microbiological penetration of the respiratory tract was observed. These data provide evidence on which to base safety protocols for use of positive pressure suits within high containment laboratories.

  14. A Microbiological Water Quality Evaluation of Ganges River Deltaic Aquifers

    Science.gov (United States)

    Yerby, C. J.; Gragg, S. E.; Page, J.; Leavens, J.; Bhattacharya, P.; Harrington, J.; Datta, S.

    2014-12-01

    Substantial natural contamination from trace elements (like arsenic) and pathogens make Ganges Deltaic aquifers an area of utmost concern. Following millions of cases of chronic arsenic poisoning from the groundwaters of the region, numerous residents are still knowingly ingesting water from shallow to intermediate accessible depth drinking water wells. Added to the calamity of arsenic is the prevalence of pathogenic bacteria in these waters. The increasing frequency of gastroenteritis signifies the need to quantify the magnitude and extensiveness of health degrading agents--bacterial pathogens (i.e. Salmonella) and non-pathogens (i.e. Enterobacteriaceae) --within the water supply in accessible Gangetic aquifers. To assess the dissolved microbiological quality in the region, present study sampling locations are along defined piezometer nests in an area in SE Asia (Bangladesh). Every nest contains samples from wells at varying depths covering shallow to deep aquifers. To date, 17 of the 76 water samples were analyzed for Salmonella, generic Escherichia coli (E. coli) and coliforms. Briefly, samples were plated in duplicate onto E. coli/Coliform petrifilm and incubated at 370C for 48 hours. Next, each sample was enriched in buffered peptone water and incubated at 370C for 18 hours. Bacterial DNA was extracted and amplified using a qPCR machine. Amplification plots were analyzed to determine presence/absence of microorganisms. All water samples (n=~76) are analyzed for Salmonella, Escherichia coli O157:H7, Listeria spp. and Shigella. Pathogen populations of PCR-positive water samples are enumerated using the agar direct plate method. Non-pathogenic bacterial indicator organisms (i.e. Enterobacteriaceae) will also be enumerated. Over the course of the experiment, we hypothesize that shallower wells will 1)have a higher pathogen prevalence and 2)harbor pathogens and nonpathogens at higher concentrations. While the 17 samples analyzed to date were negative for Salmonella

  15. Construction and Evaluation of an Online Microbiology Course for Nonscience Majors

    OpenAIRE

    Lee Hughes

    2008-01-01

    The development of web-based technologies provides a new method for course delivery. As with any new technique, evaluation is a necessary tool to determine if the method is consistent with expectations. This study describes the conversion of a nonscience majors’ microbiology lecture course to online delivery and evaluates the hypothesis that the online course can be as effective as the traditional course. Course examination scores are compared between the face-to-face and online section...

  16. Evaluation of cephamycins as supplements to selective agar for detecting Campylobacter spp. in chicken carcass rinses.

    Science.gov (United States)

    Chon, Jung-Whan; Kim, Young-Ji; Kim, Hong-Seok; Kim, Dong-Hyeon; Kim, Hyunsook; Song, Kwang-Young; Sung, Kidon; Seo, Kun-Ho

    2016-04-16

    Although cefoperazone is the most commonly used antibiotic in Campylobacter-selective media, the distribution of cefoperazone-resistant bacteria such as extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli is increasing. Here we evaluated the potential of cephamycins for use as supplements to improve modified charcoal-cefoperazone-deoxycholate agar (mCCDA) by replacing cefoperazone with the same concentrations (32 mg/L) of cefotetan (modified charcoal-cefotetan-deoxycholate agar, mCCtDA) and cefoxitin (modified charcoal-cefoxitin-deoxycholate agar, mCCxDA). In chicken carcass rinse samples, the number of mCCDA plates detecting for Campylobacter (18/70, 26%) was significantly lower than that of mCCtDA (42/70, 60%) or mCCxDA plates (40/70, 57%). The number of mCCDA plates (70/70, 100%) that were contaminated with non-Campylobacter species was significantly higher than that of mCCtDA (20/70, 29%) or mCCxDA plates (21/70, 30%). The most common competing species identified using mCCDA was ESBL-producing E. coli, while Pseudomonas species frequently appeared on mCCtDA and mCCxDA.

  17. Effects of single nucleotide polymorphism marker density on degree of genetic variance explained and genomic evaluation for carcass traits in Japanese Black beef cattle

    OpenAIRE

    Ogawa, Shinichiro; Matsuda, Hirokazu; Taniguchi, Yukio; Watanabe, Toshio; Nishimura, Shota; Sugimoto, Yoshikazu; Iwaisaki, Hiroaki

    2014-01-01

    [Background]Japanese Black cattle are a beef breed whose meat is well known to excel in meat quality, especially in marbling, and whose effective population size is relatively low in Japan. Unlike dairy cattle, the accuracy of genomic evaluation (GE) for carcass traits in beef cattle, including this breed, has been poorly studied. For carcass weight and marbling score in the breed, as well as the extent of whole genome linkage disequilibrium (LD), the effects of equally-spaced single nucleoti...

  18. Effects of single nucleotide polymorphism marker density on degree of genetic variance explained and genomic evaluation for carcass traits in Japanese Black beef cattle

    OpenAIRE

    Ogawa, Shinichiro; Matsuda, Hirokazu; Taniguchi, Yukio; Watanabe, Toshio; Nishimura, Shota; Sugimoto, Yoshikazu; Iwaisaki, Hiroaki

    2014-01-01

    Background Japanese Black cattle are a beef breed whose meat is well known to excel in meat quality, especially in marbling, and whose effective population size is relatively low in Japan. Unlike dairy cattle, the accuracy of genomic evaluation (GE) for carcass traits in beef cattle, including this breed, has been poorly studied. For carcass weight and marbling score in the breed, as well as the extent of whole genome linkage disequilibrium (LD), the effects of equally-spaced single nucleotid...

  19. Evaluation of Tazobactam-Supplemented, Modified Charcoal-Cefoperazone-Deoxycholate Agar for Qualitative Detection of Campylobacter from Chicken Carcass Rinse.

    Science.gov (United States)

    Chon, Jung-Whan; Kim, Young-Ji; Kim, Hong-Seok; Kim, Dong-Hyeon; Jeong, Dong Kwan; Seo, Kun-Ho

    2016-05-01

    Overgrowth of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli on modified charcoal-cefoperazone-deoxycholate agar (mCCDA) is the most common confounding factor for the isolation of Campylobacter from poultry samples. mCCDA modified by supplementation with tazobactam, an ESBL inhibitor, was evaluated for Campylobacter isolation from chicken carcass rinse with regard to isolation rate and selectivity. In total, 120 whole chicken carcasses purchased from retail stores were rinsed with buffered peptone water enriched with 2× blood-free Bolton broth at 42°C for 48 h and then inoculated onto mCCDA with and without tazobactam supplementation (mCCDA or T-mCCDA) at 42°C for 48 h under microaerobic conditions. Suspect colonies were subcultured and confirmed by colony PCR. Plates with tazobactam exhibited a higher Campylobacter isolation rate (56.7% vs. 30.8%, p < 0.05) and selectivity (0.8 vs. 83.3% plates contaminated with non-Campylobacter, p < 0.05) than mCCDA. Thus, tazobactam-supplemented mCCDA would be a useful option for qualitative detection of Campylobacter in chicken carcass rinse.

  20. Parasitological and microbiological evaluation of Mixe Indian medicinal plants (Mexico).

    Science.gov (United States)

    Heinrich, M; Kuhnt, M; Wright, C W; Rimpler, H; Phillipson, J D; Schandelmaier, A; Warhurst, D C

    1992-02-01

    Medicinal plants are an important health resource in many regions of the Americas and are of particular importance to many Indian communities. Based on a recent ethnobotanical study in Mexico, we investigated the activity of 29 plant extracts against Entamoeba histolytica, three bacteria (Bacillus subtilis, Escherichia coli, and Micrococcus luteus) and two fungi (Cladosporium cucumerinum and Penicillium oxalicum). After separation of these extracts between CH2Cl2 and H2O the resulting phases were also evaluated. PMID:1501496

  1. Sensory and microbiological evaluation of uncured fresh chicken sausage with reduced fat content

    OpenAIRE

    Anna Cecilia Venturini; Ângela Dulce Cavenaghi; Carmen Josefina Contreras Castillo; Eliane Marta Quiñones

    2011-01-01

    The purpose of this study was to evaluate the acceptability and the microbiological safety of uncured fresh chicken sausages with reduced fat content, considering the scientific evidence that correlated cancer and cardiovascular diseases to this diet. Two formulations of uncured fresh chicken sausage were processed using different concentrations of cochineal carmine pigment, rosemary extracts and synthetic antioxidants, which are used to give color, appearance and pleasant flavor to the produ...

  2. Engineering Your Own Superbug: A Useful Assignment to Evaluate Real Learning Comprehension in Microbiology Classes †

    OpenAIRE

    Yun-Chi Chen

    2013-01-01

    The learning comprehension of students in microbiology classes is not easy to evaluate, particularly when the student population is diverse in terms of backgrounds, majors, levels of training and preparedness, and the students’ expectations and enthusiasm for the class. It is difficult to design a one-size-fits-all exam that best suits a mixed student population; and most traditional assignments/case studies focusing on particular microbes or topics might not readily assess students’ overall ...

  3. Physicochemical and microbiological evaluation of corrientes artisanal cheese during ripening

    Directory of Open Access Journals (Sweden)

    Olga Myriam Vasek

    2013-03-01

    Full Text Available The aim of this study was to evaluate some physical and chemical parameters (total solids, pH, acidity, fat, acid degree value of fat, salt, protein and nitrogen fractions and their effects on the beneficial (lactic acid bacteria: LAB and undesirable microbial populations (coliforms, Escherichia coli, Staphylococcus aureus, moulds, and yeast during ripening of Artisanal Corrientes Cheese, an Argentinian cow's milk variety, to determine whether a longer ripening period than usual improve its hygienic-sanitary quality. The protein content was much higher than that of other cow's milk cheeses with similar values of fat. The larger peptides showed values three times higher in the 30 day-old cheese than those obtained in the beginning of the process. Staphylococcus aureus and Escherichia coli were detected (3.04 ± 1.48 log10 cfu/g of cheese, 2.21 ± 0.84 log10 MPN/g of cheese even at 15 and 30 days of ripening, respectively. The distribution of three hundred LAB strains classified to the genus level (lactococci:lactobacilli:leuconostocs was maintained during the ripening period. The high number of LAB in rennet may have contributed to the fermentation as a natural whey starter, unknown source of LAB for this specific cheese so far. The physicochemical changes that occur during ripening were not big enough to inhibit the growth of undesirable microorganisms.

  4. A Bayesian approach to the evaluation of risk-based microbiological criteria for Campylobacter in broiler meat

    OpenAIRE

    Ranta, Jukka; Lindqvist, Roland; Hansson, Ingrid; Tuominen, Pirkko; Nauta, Maarten

    2015-01-01

    Shifting from traditional hazard-based food safety management toward risk-based management requires statistical methods for evaluating intermediate targets in food production, such as microbiological criteria (MC), in terms of their effects on human risk of illness. A fully risk-based evaluation of MC involves several uncertainties that are related to both the underlying Quantitative Microbiological Risk Assessment (QMRA) model and the production-specific sample data on the prevalence and con...

  5. Microbiological and sensory evaluation of Jambu (Acmella oleracea L. dried by cold air circulation

    Directory of Open Access Journals (Sweden)

    Alan Franco BARBOSA

    2016-01-01

    Full Text Available Abstract The aim of this study was to evaluate the microbiological and sensory quality of the Jambu (Acmella oleracea L. in natura and dried by cold air, and the determination of its drying curve. The microbiological analysis were performed to Salmonella spp, the coagulase-positive Staphylococcus, and coliforms in the both Jambu samples, at 45 °C. Tacacá, the typical food dish of Pará state, Brazil, has showed good consumer global acceptance in the sensory evaluation of Jambu in natura (score of 8.00 ± 1.46 and dried (score of 8.67 ± 0.66. Both samples, Jambu in natura and dried by cold air, were by the current legislation regarding the microbiological aspects, this is the absence of Salmonella spp, coagulase-positive Staphylococcus <1×101 CFU/g, and coliforms <3 MPN/g, at 45 °C. Thus, considering sensory and health aspects, the commercialization of dried Jambu becomes viable, facilitating its transportation and handling, as well as for reducing its vegetable mass.

  6. In vitro microbiological evaluation of polyvinyl alcohol-based ocular inserts of Ciprofloxacin hydrochloride

    Directory of Open Access Journals (Sweden)

    Balasubramaniam J

    2006-01-01

    Full Text Available Soluble inserts of ciprofloxacin hydrochloride using high and low molecular weight polyvinyl alcohol alone and in various combinations were fabricated by a casting technique. The in vitro drug release from the prepared inserts was studied using a continuous flow-through model, developed in our laboratory. The antimicrobial efficacies of the prepared inserts against common ocular pathogens, viz., Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853, were evaluated using a modified in vitro microbiological model. Ciprofloxacin hydrochloride release from the inserts followed matrix diffusion kinetics showing an anomalous release mechanism (erosion-controlled based on the calculated release exponent (n values. Drug release increased with an increase in the proportion of high molecular weight polyvinyl alcohol in the inserts. The in vitro microbiological model demonstrated the effectiveness of the inserts against the two microorganisms. Further, the results of the in vitro release studies correlated well with that of the antimicrobial studies.

  7. Microbiological evaluation of milk samples positive to California Mastitis Test in dairy buffalo cows (Buballus bubalis

    Directory of Open Access Journals (Sweden)

    D.J. Sturion

    2010-02-01

    Full Text Available In order to observe the microbiological status of CMT positive samples, 734 apparently health mammary quarters from buffalo cows were submitted to physical evaluation, strip cup test and CMT. After milk samples inoculation in 10% ovine blood agar base media and in MacConkey agar and incubation under aerobic condition for 72 hours at 37oC, identification was proceeded. According to CMT, 227 quarters (30,93% were positive, among them 73 (32,16% presented 1+ reaction, 53 (23,35% were 2+ and 101 (44,49% were 3+. Microbiological exams of such samples were positive in 147 (64,76% out of 227 CMT positive samples and among the remaining 72 (31,72% were negative and 8 (3,52 were contaminated. In the 147 microbiological positive samples 204 bacteria were found in pure or associated growth and the most frequent agents were: Corynebacterium sp (59,25%; Staphylococcus sp (17,65% among which 86,11% were coagulase negative and 13,89% were coagulase positive; and Micrococcus sp (6,37%. The results revealed that, excluding the eight contaminated samples, 147 (67,12% quarters out of 219 CMT positive could be considered as bacteria-carrier and that even in a smaller percentage false-positive results can cause problems in a sanitary program for mastitis control in dairy buffalo cows.

  8. Evaluation of the microbiological quality of ricotta cheese commercialized in Santa Catarina, Brazil

    Directory of Open Access Journals (Sweden)

    Edailson Luís Xavier GUATEMIM

    2016-01-01

    Full Text Available Abstract Ricotta cheese has low fat and salt contents and a high quantity of proteins. The diversity of the nutrients and the high water content are factors that favor the development of microorganisms in this product. In this context, the aim of this study was to evaluate the microbiological quality of ricotta commercialized in the western region of Santa Catarina State, Brazil. Thirty samples, comprising 10 different brands of ricotta, were collected and sent to the Food Microbiology Laboratory at the Instituto Federal Catarinense (IFC, Campus Concórdia for microbiological analysis to determine the presence of Salmonella sp., Listeria sp., coagulase-positive Staphylococcus and thermotolerant coliforms. Of the 30 samples, the results for 33.3% were not within the parameters established by current legislation, that is, they were not fit for human consumption. This high percentage was due to contamination by thermotolerant coliforms, with 10 samples containing this group of microorganisms. One sample was contaminated with Listeria grayi, and for the other microorganisms investigated, (Salmonella sp. and coagulase-positive Staphylococcus the results were satisfactory. This study characterizes the hygiene-sanitary conditions of ricotta commercialized in the western region of Santa Catarina State, highlighting the need for greater care during the production of this product.

  9. Shelf life of anchovy products (Engraulis encrasicolus: evaluation of sensory, microbiological and chemical properties

    Directory of Open Access Journals (Sweden)

    Andrea Ariano

    2014-02-01

    Full Text Available Fishery products have always been an important food in Italy. In the past, increased consumption was mainly due to the good quality of the products, easiness of use and their beneficial effects on health. Recently, owing to the national financial crisis, there has been a decline in the consumption of fish. In fact, in 2013, according to data from ISMEA, the consumption of fresh fish suffered a sharp contraction (-5%. This decline also concerns anchovy (Engraulis encrasicolus. This species, partly because of its low price, is a mainstay of traditional Italian food. The aim of this study was to evaluate sensorial, chemical and microbiological properties of anchovy-based (Engraulis encrasicolus products during storage at 4 and -20°C. Fresh anchovies, obtained from the wholesale fish market of Pozzuoli (Southern Italy were cut into small pieces and hand-prepared using bread, eggs, cheese and lemon juice. Samples were analysed after 0, 2, 4, 6 and 8 days of storage at 4°C. An aliquot was quickly frozen and analysed after 34 days at -20°C. Sensory assessment, microbiological (specific spoilage organisms, Listeria spp. and Salmonella spp. and chemical (histamine, total volatile basic nitrogen, trimethylamine, thiobarbituric acid, pH and aw analyses were performed. Results showed that the shelf life of anchovy products was less than 5 days for the samples stored at 4°C. At -20°C, all anchovies preparations showed good sensory, microbiological and chemical properties for 34 days.

  10. Evaluation of carcass, live, and real-time ultrasound measures in feedlot cattle: I. Assessment of sex and breed effects.

    Science.gov (United States)

    Hassen, A; Wilson, D E; Rouse, G H

    1999-02-01

    Carcass and live-animal measures from 1,029 cattle were collected at the Iowa State University Rhodes and McNay research farms over a 6-yr period. Data were from bull, heifer, and steer progeny of composite, Angus, and Simmental sires mated to three composite lines of dams. The objectives of this study were to estimate genetic parameters for carcass traits, to evaluate effects of sex and breed of sire on growth models (curves), and to suggest a strategy to adjust serially measured data to a constant age end point. Estimation of genetic parameters using a three-trait mixed model showed differences between bulls and steers in estimates of h2 and genetic correlations. Heritability for carcass weight, percentage of retail product, retail product weight, fat thickness, and longissimus muscle area from bull data were .43, .04, .46, .05, and .21, respectively. The corresponding values for steer data were in order of .32, .24, .40, .42, and .07, respectively. Analysis of serially measured fat thickness, longissimus muscle area, body weight, hip height, and ultrasound percentage of intramuscular fat using a repeated measures model showed a limitation in the use of growth models based on pooled data. In further evaluation of regression parameters using a linear mixed model analysis, sex and breed of sire showed an important (P < .05) effect on intercept and slope values. Regression of serially measured traits on age within animal showed a relatively larger R2 (62 to 98%) and a smaller root mean square error (RMSE, .09 to 8.85) as compared with R2 (0 to 58%) and RMSE (.31 to 67.9) values when the same model was used on pooled data. We concluded that regression parameters from a within-animal regression of a serially measured trait on age, averaged by sex and breed, are the best choice in describing growth and adjusting data to a constant age end point. PMID:10100654

  11. Evaluating the Efficacy of Three U.S. Department of Agriculture-Approved Antimicrobial Sprays for Reducing Shiga Toxin-Producing Escherichia coli Surrogate Populations on Bob Veal Carcasses.

    Science.gov (United States)

    2016-06-01

    Effective antimicrobial intervention strategies to reduce Shiga toxin-producing Escherichia coli (STEC) risks associated with veal are needed. This study evaluated the efficacy of lactic acid (4.5%, pH 2.0), Citrilow (pH 1.2), and Beefxide (2.25%, pH 2.3) for reducing STEC surrogates on prerigor and chilled bob veal carcasses and monitored the effects of these interventions on chilled carcass color. Dehided bob veal carcasses were inoculated with a five-strain cocktail of rifampin-resistant, surrogate E. coli bacteria.E. coli surrogates were enumerated after inoculation, after water wash, after prechill carcass antimicrobial spray application, after chilling for 24 h, and after postchill carcass antimicrobial spray application; carcass color was measured throughout the process. A standard carcass water wash (∼50°C) reduced the STEC surrogate population by 0.9 log CFU/cm(2) (P ≤ 0.05). All three antimicrobial sprays applied to prerigor carcasses delivered an additional ∼0.5-log reduction (P ≤ 0.05) of the surrogates. Chilling of carcasses for 24 h reduced (P ≤ 0.05) the surrogate population by an additional ∼0.4 log cycles. The postchill application of the antimicrobial sprays provided no further reductions. Carcass L*, a*, and b* color values were not different (P > 0.05) among carcass treatments. Generally, the types and concentrations of the antimicrobial sprays evaluated herein did not negatively impact visual or instrumental color of chilled veal carcasses. This study demonstrates that warm water washing, followed by a prechill spray treatment with a low-pH chemical intervention, can effectively reduce STEC risks associated with veal carcasses; this provides processors a validated control point in slaughter operations.

  12. Evaluating the Efficacy of Three U.S. Department of Agriculture-Approved Antimicrobial Sprays for Reducing Shiga Toxin-Producing Escherichia coli Surrogate Populations on Bob Veal Carcasses.

    Science.gov (United States)

    2016-06-01

    Effective antimicrobial intervention strategies to reduce Shiga toxin-producing Escherichia coli (STEC) risks associated with veal are needed. This study evaluated the efficacy of lactic acid (4.5%, pH 2.0), Citrilow (pH 1.2), and Beefxide (2.25%, pH 2.3) for reducing STEC surrogates on prerigor and chilled bob veal carcasses and monitored the effects of these interventions on chilled carcass color. Dehided bob veal carcasses were inoculated with a five-strain cocktail of rifampin-resistant, surrogate E. coli bacteria.E. coli surrogates were enumerated after inoculation, after water wash, after prechill carcass antimicrobial spray application, after chilling for 24 h, and after postchill carcass antimicrobial spray application; carcass color was measured throughout the process. A standard carcass water wash (∼50°C) reduced the STEC surrogate population by 0.9 log CFU/cm(2) (P ≤ 0.05). All three antimicrobial sprays applied to prerigor carcasses delivered an additional ∼0.5-log reduction (P ≤ 0.05) of the surrogates. Chilling of carcasses for 24 h reduced (P ≤ 0.05) the surrogate population by an additional ∼0.4 log cycles. The postchill application of the antimicrobial sprays provided no further reductions. Carcass L*, a*, and b* color values were not different (P > 0.05) among carcass treatments. Generally, the types and concentrations of the antimicrobial sprays evaluated herein did not negatively impact visual or instrumental color of chilled veal carcasses. This study demonstrates that warm water washing, followed by a prechill spray treatment with a low-pH chemical intervention, can effectively reduce STEC risks associated with veal carcasses; this provides processors a validated control point in slaughter operations. PMID:27296599

  13. Microbiological evaluation of milk samples positive to California Mastitis Test in dairy buffalo cows (Buballus bubalis)

    OpenAIRE

    D.J. Sturion; D.L. Ravena; M.E.A. Oliveira; E.B.S. Ferreira; H Tonhati; H Langoni; Taís A.B. Santos; A. Nader Filho; R. Bonini Pardo; G. Mendoza-Sánchez

    2010-01-01

    In order to observe the microbiological status of CMT positive samples, 734 apparently health mammary quarters from buffalo cows were submitted to physical evaluation, strip cup test and CMT. After milk samples inoculation in 10% ovine blood agar base media and in MacConkey agar and incubation under aerobic condition for 72 hours at 37oC, identification was proceeded. According to CMT, 227 quarters (30,93%) were positive, among them 73 (32,16%) presented 1+ reaction, 53 (23,35%) were 2+ and 1...

  14. Engineering Your Own Superbug: A Useful Assignment to Evaluate Real Learning Comprehension in Microbiology Classes

    Directory of Open Access Journals (Sweden)

    Yun-Chi Chen

    2013-08-01

    Full Text Available The learning comprehension of students in microbiology classes is not easy to evaluate, particularly when the student population is diverse in terms of backgrounds, majors, levels of training and preparedness, and the students’ expectations and enthusiasm for the class. It is difficult to design a one-size-fits-all exam that best suits a mixed student population; and most traditional assignments/case studies focusing on particular microbes or topics might not readily assess students’ overall understanding and learning comprehension. It is important to develop an assessment method that not only can engage students in active learning and deliberate practice but can also promote their imaginative and creative potential. The word “superbugs” often appears in the media and refers to some deadly or drug-resistant microbes. These superbugs possess special phenotypic and functional attributes that constitute their “superness.” It is predicted that more new surprising superbugs will emerge in the future and students should be challenged now with some mindstimulating ideas and exercises in their microbiology class. To develop a supplementary tool to evaluate students’ comprehension and to prepare them for the predicted superbugs unknown to us, a writing project entitled “Constructing Your Own Superbug” was designed to achieve these goals.

  15. Evaluation of Physicochemical, Microbiological and Sensory Stability of Frozen Stored Vacuum-Packed Lamb Meat

    Institute of Scientific and Technical Information of China (English)

    Rafaella de Paula Paseto Fernandes; Maria Teresa de Alvarenga Freire; Celso da Costa Carrer; Marco Antonio Trindade

    2013-01-01

    Nowadays, lamb meat represents only 7% of all meat produced in the world. In recent years the demand for standardized lamb meat cuts has been considered of great importance and the marketing occurs predominantly in the form of frozen cuts. Herewith, the main of this work was to evaluate the stability and safety of lamb meat during frozen storage. Meats were vacuum packed in high barrier multilayer plastic iflms and stored during 12 mon at (-18±1)ºC. The meat stability was assessed by physical and chemical (lipid oxidation, objective color, pH value, cooking losses and instrumental texture), microbiological (total count of psychrotrophic, coliform count at 45°C, coagulase-positive staphylococci and the presence of Salmonella) and sensory analysis (acceptance test and visual evaluation). The vacuum packed lamb meat remained stable as to most physical and chemical indexes. Microbiological indexes showed good stability throughout the storage period according to Brazilian legislation standards to pathogenic microorganisms. Although a signiifcant reduction in tenderness (shear force increase from 3 to 8 kg), it showed a good sensorial acceptance for all attributes tested, including texture, with scores of around 7 (like moderately) during the 12 mon of storage. Therefore, it can be concluded that, under the conditions applied in this study, lamb meat presents a shelf life of at least 12 mon when stored at-18°C.

  16. Evaluation of Columbia, USMARC-Composite, Suffolk, and Texel rams as terminal sires in an extensive rangeland production system: V. Postfabrication carcass component weights

    Science.gov (United States)

    Postfabrication carcass component weights of 517 crossbred wether lambs were analyzed to evaluate 4 terminal-sire breeds. Wethers were produced over 3 yr from single-sire matings of 22 Columbia, 22 USMARC-Composite (Composite), 21 Suffolk, and 17 Texel rams to adult Rambouillet ewes. Lambs were rais...

  17. Evaluation of response to bovine viral diarrhea virus type 2 vaccination and timing of weaning on yearling ultrasound body composition, performance, and carcass quality traits in Angus calves

    Science.gov (United States)

    There are concerns about antagonisms between immunity and animal productivity in livestock production. The objective of this study was to evaluate the effect of antibody levels through a response to vaccination protocol, weaning timing, and their interaction on performance and carcass quality traits...

  18. Nutritional and microbiological evaluations of chocolate-coated Chinese chestnut (Castanea mollissima) fruit for commercial use

    Institute of Scientific and Technical Information of China (English)

    Mahamadou E.GOUNGA; Shi-ying XU; Zhang WANG

    2008-01-01

    In recent years, China has become an increasingly important and the largest chestnut producer in the world. This study aimed to evaluate the nutritional value and microbiological quality of the roasted freeze-dried Chinese chestnut (Castanea mollissima) (RFDC) coated with dark chocolate (DCC) and milk chocolate (MCC) for industrial use and commercial consumption.Chocolate coating significantly improved the nutritional value of chestnut. RFDC had high levels of starch (66.23%) and fibers (3.85%) while DCC and MCC contained significantly high amounts of sucrose, protein, fat and minerals. Furthermore, the protein content doubled in MCC rather than in DCC. This could be attributed to the different formulations in the two products. Milk powder and whey protein constituted the source of protein in MCC while cocoa powder added to MCC formulation constituted an additional source of minerals. The amino acid profile showed differences in amino acid composition related to the sample's protein content, indicating their good nutritional quality. The moisture contents in all RFDC, DCC and MCC were suitable for industrial processing. These results provide information about the additional nutrients of chocolate-coated chestnut and confirm that the product is an interesting nutritional food. The combination of freeze-drying and chocolate-coating generally results in greater reductions on microbiological loads, extending shelf life of harvested chestnut for commercial application. This is an alternative strategy to add value to chestnut, minimizing the significant losses in harvested fruits and providing a wider range of choices of new products to the consumer disposal.

  19. Microbiological evaluation of bottled non-carbonated ("still") water from domestic brands in Greece.

    Science.gov (United States)

    Venieri, D; Vantarakis, A; Komninou, G; Papapetropoulou, M

    2006-03-01

    The microbiological quality of 1,527 samples of bottled non-carbonated ('still') mineral water, purchased from retail outlets and derived from 10 manufacturing companies in Greece, was investigated during the period 1995-2003. Applying the membrane filter technique, the aliquots of water samples (250 ml) were analyzed for the presence and enumeration of total coliforms, Escherichia coli, Enterococcus spp. and Pseudomonas aeruginosa. Also, aerobic bacteria were counted as Heterotrophic Plate Count (HPC) ml(-1) at 22 and 37 degrees C. Positive samples for the parameters tested varied significantly among brands with an overall percentage of 13.95% bottled water samples noncompliant with the Greek water regulation. Microorganisms isolated from the samples tested were identified as species of Pseudomonas, Aeromonas, Pasteurella, Citrobacter, Flavobacterium, Providencia and Enterococcus. The most frequent isolated microorganism during the period of the study was P. aeruginosa. Generally, bacterial load of the samples tested ranged in low levels. The purpose of the current study was to evaluate the microbiological quality of the bottled water provided by domestic brands in the Greek market during the period 1995-2003. PMID:16271413

  20. A Bayesian approach to the evaluation of risk-based microbiological criteria for Campylobacter in broiler meat

    DEFF Research Database (Denmark)

    Ranta, Jukka; Lindqvist, Roland; Hansson, Ingrid;

    2015-01-01

    Shifting from traditional hazard-based food safety management toward risk-based management requires statistical methods for evaluating intermediate targets in food production, such as microbiological criteria (MC), in terms of their effects on human risk of illness. A fully risk-based evaluation...... of MC involves several uncertainties that are related to both the underlying Quantitative Microbiological Risk Assessment (QMRA) model and the production-specific sample data on the prevalence and concentrations of microbes in production batches. We used Bayesian modeling for statistical inference...

  1. Selection Indices and Multivariate Analysis Show Similar Results in the Evaluation of Growth and Carcass Traits in Beef Cattle.

    Directory of Open Access Journals (Sweden)

    Fernando Brito Lopes

    Full Text Available This research evaluated a multivariate approach as an alternative tool for the purpose of selection regarding expected progeny differences (EPDs. Data were fitted using a multi-trait model and consisted of growth traits (birth weight and weights at 120, 210, 365 and 450 days of age and carcass traits (longissimus muscle area (LMA, back-fat thickness (BF, and rump fat thickness (RF, registered over 21 years in extensive breeding systems of Polled Nellore cattle in Brazil. Multivariate analyses were performed using standardized (zero mean and unit variance EPDs. The k mean method revealed that the best fit of data occurred using three clusters (k = 3 (P < 0.001. Estimates of genetic correlation among growth and carcass traits and the estimates of heritability were moderate to high, suggesting that a correlated response approach is suitable for practical decision making. Estimates of correlation between selection indices and the multivariate index (LD1 were moderate to high, ranging from 0.48 to 0.97. This reveals that both types of indices give similar results and that the multivariate approach is reliable for the purpose of selection. The alternative tool seems very handy when economic weights are not available or in cases where more rapid identification of the best animals is desired. Interestingly, multivariate analysis allowed forecasting information based on the relationships among breeding values (EPDs. Also, it enabled fine discrimination, rapid data summarization after genetic evaluation, and permitted accounting for maternal ability and the genetic direct potential of the animals. In addition, we recommend the use of longissimus muscle area and subcutaneous fat thickness as selection criteria, to allow estimation of breeding values before the first mating season in order to accelerate the response to individual selection.

  2. Selection Indices and Multivariate Analysis Show Similar Results in the Evaluation of Growth and Carcass Traits in Beef Cattle

    Science.gov (United States)

    Brito Lopes, Fernando; da Silva, Marcelo Corrêa; Magnabosco, Cláudio Ulhôa; Goncalves Narciso, Marcelo; Sainz, Roberto Daniel

    2016-01-01

    This research evaluated a multivariate approach as an alternative tool for the purpose of selection regarding expected progeny differences (EPDs). Data were fitted using a multi-trait model and consisted of growth traits (birth weight and weights at 120, 210, 365 and 450 days of age) and carcass traits (longissimus muscle area (LMA), back-fat thickness (BF), and rump fat thickness (RF)), registered over 21 years in extensive breeding systems of Polled Nellore cattle in Brazil. Multivariate analyses were performed using standardized (zero mean and unit variance) EPDs. The k mean method revealed that the best fit of data occurred using three clusters (k = 3) (P < 0.001). Estimates of genetic correlation among growth and carcass traits and the estimates of heritability were moderate to high, suggesting that a correlated response approach is suitable for practical decision making. Estimates of correlation between selection indices and the multivariate index (LD1) were moderate to high, ranging from 0.48 to 0.97. This reveals that both types of indices give similar results and that the multivariate approach is reliable for the purpose of selection. The alternative tool seems very handy when economic weights are not available or in cases where more rapid identification of the best animals is desired. Interestingly, multivariate analysis allowed forecasting information based on the relationships among breeding values (EPDs). Also, it enabled fine discrimination, rapid data summarization after genetic evaluation, and permitted accounting for maternal ability and the genetic direct potential of the animals. In addition, we recommend the use of longissimus muscle area and subcutaneous fat thickness as selection criteria, to allow estimation of breeding values before the first mating season in order to accelerate the response to individual selection. PMID:26789008

  3. A microbiological evaluation of SiO2-coated textiles in hospital interiors

    DEFF Research Database (Denmark)

    Mogensen, Jeppe; Jørgensen, Poul-Erik; Thomsen, Trine Rolighed

    2016-01-01

    The use of passive coatings could be a new solution to improve the cleaning potential of interior textiles in hospitals. In these years, the scepticism toward the use of antibacterial textiles in the health care sector is emerging, and in the Nordic countries, the implementation success is confined....... From this perspective, the purpose of this paper is therefore to address focus on alternative passive coatings that without actively killing the bacteria provide a hydrophobic and easy-to-clean textile surface. The paper relates to an in-situ study evaluating the effect and cleaning potential of SiO2......-coated textiles compared to traditional textiles and a hard plastic surface as a reference material. Through the study, arranged at an outpatient lung department at Hospital Vendsyssel, Denmark, five different surface materials were installed on hospital chair armrests and sampled with microbiological...

  4. Evaluation of low-dose irradiation on microbiological quality of white carrots and string beans

    International Nuclear Information System (INIS)

    The minimally processed food provided the consumer with a product quality, safety and practicality. However, minimal processing of food does not reduce pathogenic population of microorganisms to safe levels. Ionizing radiation used in low doses is effective to maintain the quality of food, reducing the microbiological load but rather compromising the nutritional values and sensory property. The association of minimal processing with irradiation could improve the quality and safety of product. The purpose of this study was to evaluate the effectiveness of low-doses of ionizing radiation on the reduction of microorganisms in minimally processed foods. The results show that the ionizing radiation of minimally processed vegetables could decontaminate them without several changes in its properties.

  5. [THE EVALUATION OF MICROBIOLOGICAL DISORDERS OF MICROFLORA OF OROPHARYNX AND INTESTINE USING MATHEMATICAL MODELING TECHNIQUE].

    Science.gov (United States)

    Zatevalov, A V; Elkova, E P; Afanasiev, S S; Aleshkin, A V; Mironov, A Yu; Gusarova, M P; Gudova, N V

    2016-02-01

    The analysis was applied to microflora of feces and oropharinx and concentration of volatile fatty acids in saliva from patients of consultative diagnostic center of G.N. Gabrichevskii Moscow research institute of epidemiology and microbiology. The computer classification program is developed on the basis of determining degree of microbiological disorders on the basis of received data and using artificial neural networks and discriminant analysis. The analysis established decreasing of probability of false classification in case of increasing of degree of microbiological disorders of microflora of intestine and absence of such a correlation for microbiological and metabolic disorders of microflora of intestine. PMID:27455568

  6. [THE EVALUATION OF MICROBIOLOGICAL DISORDERS OF MICROFLORA OF OROPHARYNX AND INTESTINE USING MATHEMATICAL MODELING TECHNIQUE].

    Science.gov (United States)

    Zatevalov, A V; Elkova, E P; Afanasiev, S S; Aleshkin, A V; Mironov, A Yu; Gusarova, M P; Gudova, N V

    2016-02-01

    The analysis was applied to microflora of feces and oropharinx and concentration of volatile fatty acids in saliva from patients of consultative diagnostic center of G.N. Gabrichevskii Moscow research institute of epidemiology and microbiology. The computer classification program is developed on the basis of determining degree of microbiological disorders on the basis of received data and using artificial neural networks and discriminant analysis. The analysis established decreasing of probability of false classification in case of increasing of degree of microbiological disorders of microflora of intestine and absence of such a correlation for microbiological and metabolic disorders of microflora of intestine.

  7. Evaluation of silage-fed biogas process performance using microbiological and kinetic methods

    Energy Technology Data Exchange (ETDEWEB)

    Jarvis, Aa.

    1996-10-01

    In this study, different kinetic and microbiological methods were used to evaluate the growth and activity of key groups of bacteria degrading ley silage in one-phase and two-phase biogas processes. Emphasis was placed on studying the dynamic behaviour of different trophic groups resulting from the initiation of liquid recirculation in the processes. The microbiological methods included microscopy and most probable number (MPN) counts with different substrates. The kinetic methods included measurements of specific methanogenic activity (SMA) with acetate and H{sub 2}/CO{sub 2} as substrates, batch assays with trace element additions and measurement of conversion rates of mannitol and lactate in the digesters. In general, the initiation of liquid recirculation at first promoted the growth and/or activity of several trophic groups of bacteria, such as butyrate and propionate degraders and acetotrophic and hydrogenotrophic methanogens in the liquefaction/acidogenesis reactors of the two-phase processes. This was probably mainly due to the increased pH. However, after some time of liquid recirculation, an inhibition of some bacterial groups occurred, such as propionate degraders and methanogens in the methanogenic reactors of two-phase processes. This was probably due to increased concentrations of salts and free ammonia. The batch assays proved to be valuable tools in process optimization by the addition of trace elements. Here, the addition of cobalt significantly increased methane production from acetate. In this study, a more comprehensive understanding of the process behaviour in response to the initiation of liquid recirculation was achieved which could not have been obtained by only monitoring routine parameters such as pH, methane production and concentrations of organic acids and salts. 120 refs, 4 figs, 1 tab

  8. Microbiological and sensory evaluation of the shelf-life of irradiated chicken breast meat

    International Nuclear Information System (INIS)

    Kraft paper boxes containing 10 expanded polystyrene trays with 200g skinless deboned chicken breast each were irradiated with 60Co source of a Nordion JS7500 irradiator. The trays were previously wrapped with polyethylene film. The samples were exposed to 1.5; 3.0 and 7.0 kGy doses in the static mode at 0º and 180º in relation to the irradiation beam. Set of 18 alanine+paraffin dosimeters per treatment were distributed inside the boxes for evaluation of irradiation dose homogeneity. A separeted dose calibration curve was obtained by irradiating in the range of 1 to 10kGy. After the irradiation, the chicken breasts were stored at 5±1ºC for 39 days and were analysed microbiologically in total psychrotrophic aerobic bacteria, total mesophilic aerobic bacteria, molds and yeasts, Pseudomonas spp, Enterobacteriaceae, lactic bacteria counts and E.coli during the storage period. The results revealed a linear behaviour of the alanine+paraffin dosimeters in the range of 1 to 10kGy irradiation. In regard to the microbiological aspect, compared to the shelf-life of 5 days for the controls, there were a increasing of 1.75; 4.40 e 7.0 times shelf-life for chicken breasts irradiated with 1.5; 3.0 and 7.0kGy, respectively. There was an increasing change of the smell of burnt as the irradiation doses increased. Thus, 3kGy dose was considered as the ideal dose to assure a longer shelf-life to the product, without perceptible changes in the aspect

  9. Microbiological evaluation of drinking water sold by roadside vendors of Delhi, India

    Science.gov (United States)

    Chauhan, Abhishek; Goyal, Pankaj; Varma, Ajit; Jindal, Tanu

    2015-07-01

    Delhi has emerged as one of the greenest capital city of the world. Microbiological assessment of drinking water emphasizes estimation of the hygienic quality of the water sold with reference to community health significance. This study was conducted to evaluate the quality of drinking water sold by roadside vendors in east, west, north and south zones of capital of India. A total number of 36 samples (nine from each zone) were collected as per national guidelines and studied for microbiological assessment. All the drinking water samples were collected in gamma-sterilized bottles and were kept in an ice pack to prevent any significant change in the microbial flora of the samples during the transportation. The water samples were transported to the laboratory in vertical position maintaining the temperature 1-4 °C with ice pack enveloped conditions. Samples were analyzed for total MPN coliform per 100 ml and for the presence and absence of common human pathogenic bacteria such as Escherichia coli, Salmonella, Staphylococcus aureus and Pseudomonas aeruginosa. All the samples were found to be contaminated with coliform organisms in the range of 14 to >1600 per 100 ml of sample. Out of 36 water samples, the occurrence of E. coli was 61 %, Salmonella 25 % S. aureus 14 % and P. aeruginosa 53 % as 22, 9, 5 and 19 samples were found contaminated, respectively. The numbers of coliform bacteria and presence of some common pathogenic bacteria suggested that the quality of drinking water sold by roadside vendors is not within the Indian standard and WHO guidelines laid down for drinking water quality. Hence, there is a vital need to study the root cause in terms of hygiene, sanitation of vendors and source of contamination to prevent waterborne diseases.

  10. Opinion of the Scientific Panel on Biological Hazards on the Evaluation of the efficacy of peroxyacids for use as an antimicrobial substance applied on poultry carcasses

    DEFF Research Database (Denmark)

    Nørrung, Birgit

    The European Commission requested EFSA to evaluate the efficacy of peroxyacids as an antimicrobial substance applied to poultry carcasses. Particularly, the BIOHAZ panel was asked to assess the efficacy of the peroxyacids on the growth and/or prevalence of some microorganisms and pathogens on pou...... and it must not be used as a substitute for normal good hygienic practice. Moreover, the experimental evaluation of the efficacy of peroxyacids must be under conditions comparable to European industrial processing conditions and practices....

  11. Comparative evaluation of carcass traits and meat quality in native Aseel chickens and commercial broilers.

    Science.gov (United States)

    Rajkumar, U; Muthukumar, M; Haunshi, S; Niranjan, M; Raju, M V L N; Rama Rao, S V; Chatterjee, R N

    2016-06-01

    A comprehensive study was conducted to analyse the meat quality attributes, composition and carcass traits in Aseel chickens and commercial broilers at market age on the basis of physiological age. A total of 20 Aseel (26 and 56 weeks) and 20 broiler (6 weeks) chickens were divided into two groups on a live weight basis, i.e. large (≥2.5 kg) and small (Commercial broilers recorded significantly higher meat proportion and lower proportion of bone. The meat:bone ratio was 1.07:1.0 in Aseel and 1.31-1.0 in broiler chicken. Breast muscle content was significantly lower in smaller Aseel chickens. Aseel chicken had stronger and heavier backs and shanks. Abdominal fat percentage was significantly lower in Aseel (0.73-0.78%). The study concluded that the firm texture of Aseel meat was due to the high collagen content and interlocking connective tissue between the muscle fibres. The texture and acceptability of Aseel meat was higher. Aseel cocks had strong legs, lean meat and less abdominal fat, making them a high-value meat bird in addition to their aggressive fighting ability.

  12. MICROBIO : A web-based program for processing and evaluation of microbiological controls on aseptic dispensing

    NARCIS (Netherlands)

    Postma, D.J.; Boom, F.A.; Bijleveld, Y.A.; Touw, D.J.; Veenbaas, T.; Essink-Tjebbes, C.M.; Verbrugge, P.

    2012-01-01

    Background: According to the Dutch GMP-hospital pharmacy directive (1) three types of microbiological controls are required when aseptic dispensing is performed: 1. microbiological monitoring of the air in the working area, the gloved hands of the operator and the work surface at the critical place

  13. Purebred-crossbred performance and genetic evaluation of postweaning growth and carcass traits in Bos indicus x Bos taurus crosses in Australia.

    Science.gov (United States)

    Newman, S; Reverter, A; Johnston, D J

    2002-07-01

    Growth and carcass data on 7,154 cattle from a purebred project and 1,241 cattle from a crossbred project, comprising 916 first-crosses and 325 purebred Brahman controls, were analyzed to estimate genetic parameters, including the genetic correlations between purebred and crossbred performance (rpc). The data also allowed the estimation of sire breed means for various growth and carcass traits. Crossbred calves were produced using 9 Angus, 8 Hereford, 7 Shorthorn, 14 Belmont Red, and 8 Santa Gertrudis sires bred to Brahman dams. These same sires produced 1,568 progeny in a separate purebreeding project. Cattle in both projects were managed under two finishing regimens (pasture and feedlot) to representative market live weights of 400 (domestic), 520 (Korean), and 600 kg (Japanese). The traits studied included live weight at around 400 d of age (400W), hot carcass weight (CWT), retail beef yield percentage (RBY), intramuscular fat percentage (IMF), rump fat depth (P8), and preslaughter ultrasound scanned eye muscle area (SEMA). Estimated breeding values (EBV) of sires from their BREEDPLAN genetic evaluations were used to assess their value in predicting crossbred performance. Regressions of actual crossbred calf performance on sire EBV for each of the traits differed little from their expectation of 0.5. Angus sires produced crossbred carcasses with the highest P8 and lowest RBY but highest IMF. In contrast, crossbred progeny from Belmont Red sires had the lightest 400W and CWT, lowest P8, and highest RBY. Estimates of rpc were 0.48, 0.48, 0.83, 0.95, 1.00, and 0.78 for 400W, CWT, RBY, IMF, P8, and SEMA, respectively. Commercial breeders selecting sires for crossbreeding programs with Brahman females, based on EBV computed from purebred data, might encounter some reranking of sire's performance for weight-related traits, with little expected change in carcass traits.

  14. Purebred-crossbred performance and genetic evaluation of postweaning growth and carcass traits in Bos indicus x Bos taurus crosses in Australia.

    Science.gov (United States)

    Newman, S; Reverter, A; Johnston, D J

    2002-07-01

    Growth and carcass data on 7,154 cattle from a purebred project and 1,241 cattle from a crossbred project, comprising 916 first-crosses and 325 purebred Brahman controls, were analyzed to estimate genetic parameters, including the genetic correlations between purebred and crossbred performance (rpc). The data also allowed the estimation of sire breed means for various growth and carcass traits. Crossbred calves were produced using 9 Angus, 8 Hereford, 7 Shorthorn, 14 Belmont Red, and 8 Santa Gertrudis sires bred to Brahman dams. These same sires produced 1,568 progeny in a separate purebreeding project. Cattle in both projects were managed under two finishing regimens (pasture and feedlot) to representative market live weights of 400 (domestic), 520 (Korean), and 600 kg (Japanese). The traits studied included live weight at around 400 d of age (400W), hot carcass weight (CWT), retail beef yield percentage (RBY), intramuscular fat percentage (IMF), rump fat depth (P8), and preslaughter ultrasound scanned eye muscle area (SEMA). Estimated breeding values (EBV) of sires from their BREEDPLAN genetic evaluations were used to assess their value in predicting crossbred performance. Regressions of actual crossbred calf performance on sire EBV for each of the traits differed little from their expectation of 0.5. Angus sires produced crossbred carcasses with the highest P8 and lowest RBY but highest IMF. In contrast, crossbred progeny from Belmont Red sires had the lightest 400W and CWT, lowest P8, and highest RBY. Estimates of rpc were 0.48, 0.48, 0.83, 0.95, 1.00, and 0.78 for 400W, CWT, RBY, IMF, P8, and SEMA, respectively. Commercial breeders selecting sires for crossbreeding programs with Brahman females, based on EBV computed from purebred data, might encounter some reranking of sire's performance for weight-related traits, with little expected change in carcass traits. PMID:12162647

  15. Evaluation of the efficiency of nested q-PCR in the detection of Mycobacterium tuberculosis complex directly from tuberculosis-suspected lesions in post-mortem macroscopic inspections of bovine carcasses slaughtered in the state of Mato Grosso, Brazil.

    Science.gov (United States)

    Carvalho, Ricardo César Tavares; Furlanetto, Leone Vinícius; Maruyama, Fernanda Harumy; Araújo, Cristina Pires de; Barros, Sílvia Letícia Bomfim; Ramos, Carlos Alberto do Nascimento; Dutra, Valéria; Araújo, Flábio Ribeiro de; Paschoalin, Vânia Margaret Flosi; Nakazato, Luciano; Figueiredo, Eduardo Eustáquio de Souza

    2015-08-01

    Bovine tuberculosis (BTB) is a zoonotic disease caused by Mycobacterium bovis, a member of the Mycobacterium tuberculosis complex (MTC). The quick and specific detection of this species is of extreme importance, since BTB may cause economic impacts, in addition to presenting imminent risks to human health. In the present study a nested real-time PCR test (nested q-PCR) was used in post-mortem evaluations to assess cattle carcasses with BTB-suspected lesions. A total of 41,193 cattle slaughtered in slaughterhouses located in the state of Mato Grosso, were examined. Of the examined animals, 198 (0.48%) showed BTB-suspected lesions. M. bovis was isolated in 1.5% (3/198) of the samples. Multiplex-PCR detected MTC in 7% (14/198) of the samples. The nested q-PCR test detected MTC in 28% (56/198) of the BTB-suspected lesions, demonstrating higher efficiency when compared to the multiplex-PCR and conventional microbiology. Nested q-PCR can therefore be used as a complementary test in the national program for control and eradication of bovine tuberculosis.

  16. Treatment of animal carcasses in poultry farms using sealed ditches.

    Science.gov (United States)

    Sánchez, M; González, J L; Gutiérrez, M A Díez; Guimaraes, A Correa; Gracia, L M Navas

    2008-10-01

    Several hen carcass elimination experiments were conducted by isolating corpses in a sealed ditch and adding different doses of lime. The aim was to evaluate the viability of this method as an alternative to other elimination techniques, as required in the European regulation CE 1774/2002 [Reglamento CE 1774/2002, de 3 de octubre por el que se establecen las normas sanitarias aplicables a los subproductos animales no destinados a consumo humano]. The experiments were carried out at a natural scale, in a 200m3 ditch located in a livestock enterprise, using a proportion of 200g of lime/kg of carcass. We observed a high degradation of carcasses after six months, the method being also safe from a microbiological point of view. The material extracted from the ditch had a high calcium content (330.7gkg(-1)), which makes it an ideal product for soil lacking this element, or as an acidity corrector in acid soils due to its basic (pH 8.48) nature. It also contains a significant amount of mineral nutrients (17.0gkg(-1) N, 2.4gkg(-1) P and 4.9gkg(-1) K) and organic matter (101.5gkg(-1)). We also analysed the material extracted from the ditch prior to its renovation for the experiments and followed the processes taking place in the ditch during the first six months, when lime doses of 100, 200 and 300 gkg(-1) of treated carcass were applied. Simultaneously, we carried out laboratory experiments in cylindrical 25L deposits to evaluate the gas release of the three (100, 200 and 300g of lime/kg carcass) doses of lime used. After the tenth week, we observed CO2 concentrations ranging from 5% for the lower lime doses to very low levels for the 300g lime/kg carcass dose. As regards methane, in the three series of experiments, the release was highest during the first weeks, began to decrease in the eighth week and reached its lower value during the fourteenth week. Emissions of NO2 were not observed, and the levels of NH3 and SH2 were usually so high that they exceeded the detection

  17. A Simple Microbiological Tool to Evaluate the Effect of Environmental Health Interventions on Hand Contamination

    Directory of Open Access Journals (Sweden)

    Carol Devamani

    2014-11-01

    Full Text Available The effects of interventions such as sanitation or hand hygiene on hand contamination are difficult to evaluate. We explored the ability of a simple microbiological test to: (1 detect recontamination after handwashing; (2 reflect risk factors for microbial contamination and (3 be applicable to large populations. The study was done in rural Andhra Pradesh, India, and Maputo, Mozambique. Participants placed all 10 fingertips on a chromogenic agar that stains Enterococcus spp. and E. coli spp. Outcomes were the number of colonies and the number of fingertips with colonies. In the recontamination study, participants were randomised to handwashing with soap and no handwashing, and tested at 30 min intervals afterwards. In two cross sectional studies, risk factors for hand contamination were explored. Recontamination of hands after washing with soap was fast, with baseline levels reached after 1 h. Child care was associated with higher Enterococcus spp. counts, whereas agricultural activities increased E. coli spp. counts. Food preparation was associated with higher counts for both organisms. In Maputo, counts were not strongly associated with water access, latrine type, education or diarrhoea. The method seems unsuitable for the evaluation of handwashing promotion. It may reflect immediately preceding risk practices but not household-level risk factors.

  18. A simple microbiological tool to evaluate the effect of environmental health interventions on hand contamination.

    Science.gov (United States)

    Devamani, Carol; Norman, Guy; Schmidt, Wolf-Peter

    2014-11-01

    The effects of interventions such as sanitation or hand hygiene on hand contamination are difficult to evaluate. We explored the ability of a simple microbiological test to: (1) detect recontamination after handwashing; (2) reflect risk factors for microbial contamination and (3) be applicable to large populations. The study was done in rural Andhra Pradesh, India, and Maputo, Mozambique. Participants placed all 10 fingertips on a chromogenic agar that stains Enterococcus spp. and E. coli spp. Outcomes were the number of colonies and the number of fingertips with colonies. In the recontamination study, participants were randomised to handwashing with soap and no handwashing, and tested at 30 min intervals afterwards. In two cross sectional studies, risk factors for hand contamination were explored. Recontamination of hands after washing with soap was fast, with baseline levels reached after 1 h. Child care was associated with higher Enterococcus spp. counts, whereas agricultural activities increased E. coli spp. counts. Food preparation was associated with higher counts for both organisms. In Maputo, counts were not strongly associated with water access, latrine type, education or diarrhoea. The method seems unsuitable for the evaluation of handwashing promotion. It may reflect immediately preceding risk practices but not household-level risk factors. PMID:25407420

  19. Sensory and microbiological evaluation of uncured fresh chicken sausage with reduced fat content

    Directory of Open Access Journals (Sweden)

    Anna Cecilia Venturini

    2011-09-01

    Full Text Available The purpose of this study was to evaluate the acceptability and the microbiological safety of uncured fresh chicken sausages with reduced fat content, considering the scientific evidence that correlated cancer and cardiovascular diseases to this diet. Two formulations of uncured fresh chicken sausage were processed using different concentrations of cochineal carmine pigment, rosemary extracts and synthetic antioxidants, which are used to give color, appearance and pleasant flavor to the products. Then, instrumental color (L*, a*, b*, C* and h*, microbial contamination and sensory tests (ranking and acceptance were used to evaluate the quality of the uncured chicken sausages. The instrumental color (chroma and hue and the sensory properties of the A and B uncured sausages were similar to the commercial cured sausage (C. However, the sensory color and appearance of samples A and B were statistically higher than those of the commercial uncured sausages D, which are prepared without the addition of nitrite or pigments. The results showed that it is possible to produce safe and high-quality uncured fresh chicken sausage with reduced fat content, using natural pigments and antioxidants.

  20. Evaluating the Impact of a Classroom Response System in a Microbiology Course

    Directory of Open Access Journals (Sweden)

    Erica Suchman

    2009-12-01

    Full Text Available The use of a Classroom Response System (CRS was evaluated in two sections, A and B, of a large lecture microbiology course. In Section B the instructor used the CRS technology at the beginning of the class period posing a question on content from the previous class. Students could earn extra credit if they answered the question correctly. In Section A, the class also began with an extra credit CRS question. However, CRS questions were integrated into the lecture during the entire class period. We compared the two classes to see if augmenting lectures with this technology increased student learning, confidence, attendance, and the instructor’s ability to respond to student’s misconceptions, over simply using the CRS as a quizzing tool. Student performance was compared using shared examination questions. The questions were categorized by how the content had been presented in class. All questions came from instructors’ common lecture content, some without CRS use, and some questions where Instructor A used both lecture and CRS questions. Although Section A students scored significantly better on both types of examination questions, there was no demonstrable difference in learning based on CRS question participation. However, student survey data showed that students in Section A expressed higher confidence levels in their learning and knowledge and indicated that they interacted more with other students than did the students in Section B. In addition, Instructor A recorded more modifications to lecture content and recorded more student interaction in the course than did Instructor B.

  1. Scientific Opinion on the evaluation of the safety and efficacy of peroxyacetic acid solutions for reduction of pathogens on poultry carcasses and meat

    Directory of Open Access Journals (Sweden)

    EFSA Panel on Biological Hazards (BIOHAZ

    2014-03-01

    Full Text Available Studies evaluating the safety and efficacy of solutions, containing peroxyacetic acid (PAA as the active ingredient, in mixtures with acetic acid, hydrogen peroxide, and 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP and possibly octanoic acid and peroxyoctanoic acid, for reduction of pathogens on poultry carcasses and meat were assessed. Treatments at ambient temperature consisted of dipping in short term baths, in chiller baths or spraying. On the basis of the previous EFSA exposure scenarios including short term baths that were not evaluated previously, no toxicity concerns were identified with regard to residues of peroxyacids, to HEDP and to possible reaction products of hydrogen peroxide and peroxyacids with lipids and proteins of the poultry carcasses. A relevant reduction of PAA treatment on E. coli and coliforms was demonstrated by dipping warm carcasses, but few data were available for pathogens (Salmonella and Campylobacter. Spraying appeared to be less effective than dipping in reducing indicator organisms than dipping. When dipping chilled carcasses, reduction of indicator organisms and pathogens was evident, although only in low or medium strength of evidence studies. In chiller bath application, there was a relevant impact on E. coli, but less effect on coliforms, and little data was available on reduction of pathogens. The emergence of acquired reduced susceptibility to biocides and/or resistance to therapeutic antimicrobials following the use of PAA was considered unlikely. There were no concerns for environmental risk of peroxyacids, acetic acid and octanoic acid. On the basis of a conservative preliminary guideline for surface water quality, the emission of HEDP from a poultry plant into the environment could not be considered safe a priori. It was recommended that HACCP plans should include monitoring of the concentration of HEDP and of the decontaminating substance in the working solution and post-marketing surveillance for

  2. Selection Indices and Multivariate Analysis Show Similar Results in the Evaluation of Growth and Carcass Traits in Beef Cattle.

    Science.gov (United States)

    Brito Lopes, Fernando; da Silva, Marcelo Corrêa; Magnabosco, Cláudio Ulhôa; Goncalves Narciso, Marcelo; Sainz, Roberto Daniel

    2016-01-01

    This research evaluated a multivariate approach as an alternative tool for the purpose of selection regarding expected progeny differences (EPDs). Data were fitted using a multi-trait model and consisted of growth traits (birth weight and weights at 120, 210, 365 and 450 days of age) and carcass traits (longissimus muscle area (LMA), back-fat thickness (BF), and rump fat thickness (RF)), registered over 21 years in extensive breeding systems of Polled Nellore cattle in Brazil. Multivariate analyses were performed using standardized (zero mean and unit variance) EPDs. The k mean method revealed that the best fit of data occurred using three clusters (k = 3) (P decision making. Estimates of correlation between selection indices and the multivariate index (LD1) were moderate to high, ranging from 0.48 to 0.97. This reveals that both types of indices give similar results and that the multivariate approach is reliable for the purpose of selection. The alternative tool seems very handy when economic weights are not available or in cases where more rapid identification of the best animals is desired. Interestingly, multivariate analysis allowed forecasting information based on the relationships among breeding values (EPDs). Also, it enabled fine discrimination, rapid data summarization after genetic evaluation, and permitted accounting for maternal ability and the genetic direct potential of the animals. In addition, we recommend the use of longissimus muscle area and subcutaneous fat thickness as selection criteria, to allow estimation of breeding values before the first mating season in order to accelerate the response to individual selection. PMID:26789008

  3. Evaluating the effects of a single copy of a mutation in the myostatin gene (c.*1232G>A) on carcass traits in crossbred lambs.

    Science.gov (United States)

    Masri, A Y; Lambe, N R; Macfarlane, J M; Brotherstone, S; Haresign, W; Bünger, L

    2011-04-01

    This study evaluated the effects of the ovine c.*1232G>A myostatin mutation (MM) on carcass traits in heterozygous crossbred lambs sired by Texel and Poll Dorset rams using ultrasound, CT scanning, carcass classification and VIA. In experiment 1, MM was associated with increased loin depth (+2.8%) and area (+3.2%). MM-carriers had significantly higher CT-estimated lean weight and proportion (2 to 4%) and muscle to bone ratio (by ~3%), in both experiments, and muscle to fat ratio (28%) in experiment 2. Muscle areas in three cross-sectional CT scans, were higher (2 to 5%) in MM-carriers. In experiment 2, fat-related measurements were significantly lower in MM-carrier lambs but this was not seen in experiment 1. A significant increase in muscle density, indicative of lower intramuscular fat, in MM-carriers shows that meat quality characteristics need attention. Carrying MM significantly decreased carcass fat scores. VIA did not detect any significant MM effects.

  4. Evaluation of Columbia, USMARC-Composite, Suffolk, and Texel rams as terminal sires in an extensive rangeland production system: IV. Postfabrication carcass component weights.

    Science.gov (United States)

    Mousel, M R; Notter, D R; Leeds, T D; Zerby, H N; Moeller, S J; Lewis, G S

    2013-05-01

    Postfabrication carcass component weights of 517 crossbred wether lambs were analyzed to evaluate 4 terminal-sire breeds. Wethers were produced over 3 yr from single-sire matings of 22 Columbia, 22 USMARC-Composite (Composite), 21 Suffolk, and 17 Texel rams to adult Rambouillet ewes. Lambs were reared to weaning in an extensive western rangeland production system and finished in a feedlot on a high-energy finishing diet. When wethers reached a mean BW of 54.4, 61.2, or 68.0 kg, they were transported to The Ohio State University abattoir for harvest. After refrigeration for approximately 24 h, chilled carcass weight (CCW) was measured, carcasses were fabricated according to Style A of Institutional Meat Purchase Specifications, and postfabrication weights were recorded. At comparable numbers of days on feed, Suffolk-sired lambs had heavier (P wholesale cut weights) and high-value trimming loss were greatest (P 0.06) flank weight. Data adjusted to comparable CCW reduced the number of significant sire-breed effects and changed sire-breed rankings of carcass component weights, for which sire breeds differed. After adjusting, Suffolk-sired lambs had lighter (P < 0.05) loins than Columbia- and Composite-sired lambs, Composite-sired lambs had heavier (P < 0.05) high-value cuts than Suffolk-sired lambs, and Suffolk- and Columbia-sired lambs had heavier (P < 0.05) necks than Texel-sired lambs. At predicted backfat thickness of 6.6 mm, Composite-sired lambs had a greater (P < 0.05) percentage of high-value cuts than Suffolk-sired lambs before but not after trimming. Producers can use these results to select terminal-sire breeds that will complement their production system and improve lamb value.

  5. Evaluation of Columbia, USMARC-Composite, Suffolk, and Texel rams as terminal sires in an extensive rangeland production system: IV. Postfabrication carcass component weights.

    Science.gov (United States)

    Mousel, M R; Notter, D R; Leeds, T D; Zerby, H N; Moeller, S J; Lewis, G S

    2013-05-01

    Postfabrication carcass component weights of 517 crossbred wether lambs were analyzed to evaluate 4 terminal-sire breeds. Wethers were produced over 3 yr from single-sire matings of 22 Columbia, 22 USMARC-Composite (Composite), 21 Suffolk, and 17 Texel rams to adult Rambouillet ewes. Lambs were reared to weaning in an extensive western rangeland production system and finished in a feedlot on a high-energy finishing diet. When wethers reached a mean BW of 54.4, 61.2, or 68.0 kg, they were transported to The Ohio State University abattoir for harvest. After refrigeration for approximately 24 h, chilled carcass weight (CCW) was measured, carcasses were fabricated according to Style A of Institutional Meat Purchase Specifications, and postfabrication weights were recorded. At comparable numbers of days on feed, Suffolk-sired lambs had heavier (P wholesale cut weights) and high-value trimming loss were greatest (P 0.06) flank weight. Data adjusted to comparable CCW reduced the number of significant sire-breed effects and changed sire-breed rankings of carcass component weights, for which sire breeds differed. After adjusting, Suffolk-sired lambs had lighter (P < 0.05) loins than Columbia- and Composite-sired lambs, Composite-sired lambs had heavier (P < 0.05) high-value cuts than Suffolk-sired lambs, and Suffolk- and Columbia-sired lambs had heavier (P < 0.05) necks than Texel-sired lambs. At predicted backfat thickness of 6.6 mm, Composite-sired lambs had a greater (P < 0.05) percentage of high-value cuts than Suffolk-sired lambs before but not after trimming. Producers can use these results to select terminal-sire breeds that will complement their production system and improve lamb value. PMID:23463555

  6. Microbiological Evaluation of Pork and Chicken By-Products in South Korea.

    Science.gov (United States)

    Chon, Jung-Whan; Jung, Hae-In; Kuk, Min; Lim, Jong-Soo; Seo, Kun-Ho; Kim, Soo-Ki

    2016-05-01

    In this study, we aimed to evaluate the microbiological risk of pork and chicken by-products by enumerating indicator bacteria (total aerobic bacteria, coliforms, and Escherichia coli) and identifying pathogens such as Campylobacter and Salmonella. The antibiotic resistance of pathogenic isolates was determined, and molecular subtyping was performed using automated repetitive sequence-based PCR (rep-PCR). Pork and chicken by-products were collected from 10 processing plants. The mean numbers of total aerobic bacteria, coliforms, and E. coli from 95 pork by-product samples and 64 chicken by-product samples were 5.1, 3.6, and 2.4 log CFU/g and 4.5, 3.0, and 1.8 log CFU/g, respectively. The numbers of indicator bacteria in the gastrointestinal tract (small intestine, large intestine, and gizzard) were significantly higher than those in other organs. Salmonella and Campylobacter species were detected in 3 and 5 of 95 pork by-product samples and in 6 and 3 of 64 chicken by-product samples, respectively. Four of 9 Salmonella isolates examined were resistant to eight antibiotics, and each of these resistant strains produced an extended-spectrum β-lactamase. Most Campylobacter isolates were resistant to tetracycline (7 of 8 strains) and quinolones (7 of 8 strains). The similarity in rep-PCR patterns among Salmonella isolates was more closely associated with serotype than with the processing plant and type of meat. Conversely, the rep-PCR patterns of Campylobacter isolates were specific to the processing plant. Our findings could help agencies develop regulations for protection from foodborne bacterial infections arising from animal by-products.

  7. MICROBIOLOGICAL EVALUATION OF THE EFFECTIVENESS OF COMMERCIALLY AVAILABLE DENTURE CLEANSING AGENTS

    Directory of Open Access Journals (Sweden)

    Chethan M D

    2011-06-01

    Full Text Available Purpose: The purpose of this study was conducted to compare and evaluate the efficacy of four chemically different immersion types of commercially available denture cleansers on recently fabricated complete dentures in healthy patients, using microbiological quantification method. Materials and Methods: Ten healthy subjects aged 60 – 70 year were selected. Chemical denture cleansers used were divided into four groups; Group I – Sodium hypochlorite solution 0.02% , Group II – Trisodium phosphate , Group III – Sodium perborate and Group IV – Chlorhexidine gluconate 0.2%. Posterior half of the tissue – bearing surface of the denture was swabbed using sterile cotton swabs at four different sites and cultured on blood agar .Net percentage reduction in the colony forming units before and after treatment with each of the above test agents on cultures from above four sites was calculated, tabulated and subjected to statistical analysis Results For all the groups the difference of means were statistically significant. The percentage reduction in streptococcus species count in log units for Groups I, II, III, and IV was found to be 28%, 16%, 10%, and 9% respectively. Conclusion: Cleansing agents were found to be effective in the following order, Sodium hypochlorite solution (0.02%, Trisodium phosphate, Sodium perborate and Chlorhexidine gluconate (0.2%. Clinical Implications: Treatment of dentures with denture cleansers significantly decreases the amount of subsequently formed plaque. The significant reduction in the number of microorganisms observed in this study suggests that the use of chemical cleansers is suitable method for cleaning dentures in geriatric patients.

  8. Evaluating hydrochemical data from shallow groundwater in Forsmark from a microbiological perspective

    Energy Technology Data Exchange (ETDEWEB)

    Hallbeck, Lotta (Microbial Analytics Sweden AB, Goeteborg (Sweden))

    2008-03-15

    Oxygen is one of the chemical species that can corrode a copper canister in a KBS-3 repository. It is therefore important to determine whether oxygen dissolved in precipitation or groundwater could reach repository depth by groundwater transport. This matter can be determined by gaining an understanding of the oxygen-consuming microbial processes that take place in shallow groundwater in the area of interest. This report evaluates hydrogeochemical data from shallow groundwater in the Forsmark area from a microbiological perspective. Hydrogeochemical data were gathered from soil pipes at depths from 1.6 to 9.6 m and from percussion-drilled boreholes having mid-point depths of between c. 30 and c. 180 m. Only a few of the percussion-drilled boreholes had packers installed. The sampled sections were therefore very long, allowing groundwater from many different depths to mix. Oxygen and oxidation-reduction potential (ORP) were measured in groundwater in soil pipes but not in percussion-drilled boreholes. The poor quality of the oxygen data made it difficult to identify the depth of origin of completely oxygen-free groundwater. Parameters that indicated ongoing anaerobic microbial processes, such as nitrite, ferrous iron, dissolved manganese, and sulphide, were found in many soil pipes. The soil pipes displayed individual chemical profiles in terms of chemical species related to microbial activity. The microbial activity could not be linked to the classes of soil pipe, i.e. recharge, discharge, or intermittent. Existing soil pipes and percussion-drilled boreholes could be used for additional sampling of microbial parameters. Such sampling would benefit from careful hypothesis-driven description of the sampling parameters and experience-guided choice of sampling methods

  9. Evaluating hydrochemical data from shallow groundwater in Laxemar from a microbiological perspective

    Energy Technology Data Exchange (ETDEWEB)

    Hallbeck, Lotta (Microbial Analytics Sweden AB, Goeteborg (Sweden))

    2008-01-15

    Oxygen is one of the chemical species that can corrode a copper canister in a KBS-3 repository. It is therefore important to determine whether oxygen dissolved in precipitation or groundwater could reach repository depth by means of groundwater transport. This can be determined by gaining an understanding of the oxygen-consuming microbial processes that take place in shallow groundwater in the area of interest. This report evaluates hydrogeochemical data from shallow groundwater in the Laxemar area from a microbiological perspective. Hydrogeochemical data were gathered from soil pipes at depths from 1.6 to 16.5 m and from percussion-drilled boreholes having mid-point depths of between 28.5 and 131 m. Only a few of the percussion-drilled boreholes had packers installed; the sampled sections were therefore very extended, allowing groundwaters from many different depths to mix. Oxygen and oxygen reduction potential (ORP) were not measured in groundwater from soil pipes or percussion drilled boreholes. The report therefore focuses on parameters that indicated ongoing anaerobic microbial processes, such as nitrite, ferrous iron, dissolved manganese, and sulphide, that were found in many soil pipes. Even though many of the soil pipes were located in similar environments and at relatively similar depths, ranging from 3.5 to 6 m, they displayed individual chemical profiles in terms of chemical species related to microbial activity. The microbial activity could not be linked to the classes of soil pipe, i.e. recharge, discharge, or intermittent. Existing soil pipes and percussion-drilled boreholes could be used for additional sampling to measure microbial parameters. Such sampling would benefit from the careful hypothesis-driven description of the sampling parameters and experience-guided choice of sampling methods

  10. Genetic parameters for EUROP carcass traits within different groups of cattle in Ireland

    NARCIS (Netherlands)

    Hickey, J.M.; Keane, M.G.; Kenny, D.A.; Cromie, A.R.; Veerkamp, R.F.

    2007-01-01

    The first objective of this study was to test the ability of systems of weighing and classifying bovine carcasses used in commercial abattoirs in Ireland to provide information that can be used for the purposes of genetic evaluation of carcass weight, carcass fatness class, and carcass conformation

  11. Evaluation of sealed containers for use in centrifuges by a dynamic microbiological test method.

    OpenAIRE

    Harper, G. J.

    1984-01-01

    A dynamic microbiological test has been used to assess the safety of sealed containers for use in laboratory centrifuges. When 26 models of containers (buckets or rotors) were examined 27% failed to contain aerosols. Some of the reasons for the failures are described.

  12. Thermophilic Campylobacter spp. in turkey samples: evaluation of two automated enzyme immunoassays and conventional microbiological techniques

    DEFF Research Database (Denmark)

    Borck, Birgitte; Stryhn, H.; Ersboll, A.K.;

    2002-01-01

    Aims: To determine the sensitivity and specificity of two automated enzyme immunoassays (EIA), EiaFoss and Minividas, and a conventional microbiological culture technique for detecting thermophilic Campylobacter spp. in turkey samples. Methods and Results: A total of 286 samples (faecal, meat...

  13. Chemical Properties, Microbiological Quality and Sensory Evaluation of Chicken and Duck Liver Paste (foie gras)

    Energy Technology Data Exchange (ETDEWEB)

    Abu-Salem, F. M.; Abu Arab, E. A.

    2010-07-01

    Liver paste or foie gras, which is a French term meaning fatty liver, was produced traditionally from goose and duck. Chickens are also used in the making of foie gras. The present study deals with the properties and quality of raw chicken and duck liver in comparison with manufactured liver paste (foie gras). Raw chicken liver contained 24.60% protein, 6.00% fat, 1.40 % ash, and 66.80% moisture. The average mineral values were 83.65, 50.75, 5.29, 1.15, 0.154, 0.683, 0.317 and 0.066 {mu}g/g of Fe, Zn, Cu, Mn, Cd, Pb, Ni, and Cr, respectively. The processing of liver paste (Foie gras) changed the composition of raw liver due to a loss in moisture, a release of fat and the addition of butter as a fat source. Chicken liver paste contained 27.8% moisture, 10.1% protein, 58.2% fat, and 0.8% ash. Mineral contents were 68.90, 40.50, 1.60, 1.1, 0.08, 0.22, 0.04 and 0.04 {mu}g/g of Fe, Zn, Cu, Mn, Cd, Pb, Ni, and Cr, respectively. The chemical, microbiological and sensory evaluation of liver paste (foie gras) manufactured from raw liver and preserved by the addition of 1000 ppm of both benzoic acid (BA) or sorbic acid (SA) and a mixture of 500 ppm of both BA plus SA with or without pasteurization at 85 degree centigrade were studied during the storage period for 9 days at 4 degree centigrade. Presumably, the mixing of liver paste (Foie gras) from chicken liver with 500 ppm of both BA plus SA and pasteurizing the product at 85 degree centigrade could be recommended for lowering thiobarbituric acid (TBA), total volatile nitrogen (TVN), peroxide value (PV), free fatty acid (FFA), ammonia, saponification value and hence for inhibiting lipid oxidation and preventing rancidity to an extent up to nine days of refrigerated storage (4 degree centigrade). This level is also recommended as a preservative agent to inhibit the bacterial deterioration of chicken liver paste (Foie gras). A sensory evaluation showed that liver paste from chicken was very acceptable from the standpoint of

  14. Microbiological evaluation of infected root canals and their correlation with pain

    Directory of Open Access Journals (Sweden)

    Nadine Luísa Soares de Lima Guimarães

    2012-01-01

    Full Text Available Introduction and objective: The aim of this study was to evaluate the endodontic microbiota of human teeth without pulp vitality presenting radiographically visible periapical lesions and its correlation with pre- and postoperative pain symptomatology. Material and methods: Sixteen young adult patients, both genders, aging from 18 to 45 years, presenting 21 single-rooted teeth with pulp necrosis and needing endodontic treatment were selected in the multidisciplinary clinic at the University of Fortaleza (UNIFOR. After crown surgical access, the ��������������root canals were embedded with 0.9% saline solution and the material from root canals was collected ���������������������������������������������� withhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhsterile paper point. The paper points were placed into Stuart transport medium and sent to the microbiology laboratory of the University of Fortaleza. Isolation and identification of bacteria were made by culture technique. The cleaning and shaping of root canals was performed by ��������������� ����� ���������������������������� crown-down technique..............................Intra-canal medication comprised calcium hydroxide mixed with chlorhexidine and after 14 days the canals were filled. Patients were asked about the occurrence of pain before treatment and 24 hours after cleaning and shaping procedures. Results: The most prevalent microbial group was Streptococcus sp. followed by Fusobacterium nucleatum, although Gram-positive cocci, non-sporulating Gram-positive bacilli, Gram-negative bacilli, pigmented Gram-negative bacilli, Veillonella, Staphylococcus aureus, Pseudomonas sp. were also frequently isolated. Conclusion: According to the results, it can be concluded that Fusobacterium

  15. Chilling of carcasses from double muscled cattle: time-temperature evolution and predictive modelling of growth of Listeria monocytogenes and Clostridium perfringens

    OpenAIRE

    Delhalle, Laurent; Collignon, Bertrand; Dehard, Sandrine; Didimo Imazaki, Pedro Henrique; Daube, Georges; Clinquart, Antoine

    2010-01-01

    The time/temperature combination during carcass chilling is of concern in order to avoid bacterial growth. The chilling speed is lower in carcasses with high muscular development such as large cattle from the Belgian Blue breed. Three slaughterhouses were selected for temperature and pH measurements during the chilling process at 6 different days on 4 half carcasses in order to obtain representative data from heavy carcasses with high muscular development. Predictive microbiology was used to ...

  16. [Evaluation of microbiological quality of seasoning purchased in the retail network].

    Science.gov (United States)

    Wójcik-Stopczyńska, Barbara; Jakubowska, Barbara; Szot, Krzysztof

    2010-01-01

    The estimation of microbiological quality of seasoning purchased in the retail network was the aim of this work. The study included five kinds of seasoning manufactured by four Polish companies. Microbiological analysis of the material included: total count of mesophilic aerobic bacteria and their spores, count of yeast and moulds and occurrence of coliforms bacteria, E. coli, Salmonella sp. and S. aureus. The results showed that the total count of mesophilic aerobic bacteria was high (10(5) -10(6) cfu x g(-1)) in the most of estimated spices and bacteria occurred mainly as spores. The low titre of coliform reduced down to 10(-2)-10(-3), occurrence of E. coli it was stated in 20% of samples of spices. However no pathogenic bacteria (Salmonella sp., S. aureus) was detected. Contamination of seasoning by yeasts and moulds was low in majority of samples and only in two samples the count of moulds was higher than 10(3) cfu x g(-1) and not answered requirements. Moulds were mainly represented by Aspergillus sp. (A. glaucus, A. niger, A. flavus) and than by Penicillium sp. and Rhizopus sp. Microbiological quality of seasoning was differentiated in dependence on the manufacturer. PMID:20803899

  17. Chemical, microbiological and sensory evaluation of mayonnaise prepared from ostrich eggs

    Directory of Open Access Journals (Sweden)

    Abou-Arab, Azza A.

    2008-12-01

    Full Text Available Ostrich eggs were evaluated for their chemical composition and mineral contents. Also, chemical, microbiological and sensory evaluation of the mayonnaise made from ostrich eggs in comparison to that made from chicken eggs were studied. Data indicated that ostrich eggs are a good source of protein (47.09 %, total lipids (45.10 %, carbohydrates (4.03 %, calcium (206.5 mg/100g, phosphorus (683.8 mg/100g, potassium (460 mg/100g, sodium (408.7 mg/100g and zinc (5.2 mg/100g. The chemical evaluation of unpasteurized and pasteurized mayonnaise indicated that mayonnaise made from ostrich eggs was more resistant to chemical spoilage due to auto-oxidation than that made from chicken eggs. In ostrich egg mayonnaise, stored unpasteurized was associated with significantly (P En este trabajo se ha evaluado la composición química y el contenido mineral de los huevos de avestruz. Asimismo se ha realizado la evaluación química, microbiológica y sensorial de la mayonesa preparada con huevos de avestruz en comparación con la preparada con huevos de gallina. Los resultados han mostrado que los huevos de avestruz son una buena fuente de proteínas (47,09 %, lípidos totales (45,10 %, carbohidratos (4,03 %, calcio (206,5 mg/100g, fósforo (683,8 mg/100g, potasio (460 mg/100g, sodio (408,7 mg/100g y zinc (5,2 mg/100g. La evaluación química de la mayonesa pasteurizada y no pasteurizada indicó que la mayonesa preparada con huevos de avestruz era más resistente al deterioro químico debido a autoxidación que la preparada con huevos de gallina. La mayonesa de huevos de avestruz almacenada sin pasteurizar fue asociada con una acidez valorable significativamente mayor (P < 0,05. Por el contrario, la acidez valorable no se alteró en las muestras pasteurizadas. Sin embargo, en la mayonesa de huevos de gallina la acidez valorable no varió ni en las muestras pasteurizadas ni en las no pasteurizadas. El grado de acidez y el valor del ácido tiobarbitúrico de las

  18. Salty Microbiology

    Science.gov (United States)

    Schneegurt, Mark A.; Wedel, Adrianne N.; Pokorski, Edward W.

    2004-01-01

    Using microbiology activities in the classroom is an effective way for teachers to address National Standards in the life sciences. However, common microbiology activities that involve swabbing doorknobs and hands are too risky due to the likelihood of culturing human pathogens. In addition, making sterile media and maintaining sterile conditions…

  19. Evaluation de differentes strategies de demantelement de la carcasse d'un avion

    Science.gov (United States)

    Sainte-Beuve, Damien

    At the time when sustainable development of our environment and our society become more and more considered, the issue of the end of life of aircraft arises. With the tightening of regulations and policies to reduce operating costs, aircraft are increasingly removed from service before the end of their operational life. However, the regulations do not only affect the operational life of the aircraft. In the future, there are great chances that the aircraft end of life will be also regulated as the car end of life. Nowadays some deserts serve as a tomb for airplanes skeletons, but what will happen in a few years? Recycling the skeleton is not an operation with very high added value, such as reselling used parts, however intelligent sorting and facing the market can increase the gain. At the level of the aircraft life cycle, recycling its materials reduces its overall impact, and also helps to reduce pollution and land use, even if the main impacts generated by planes are created during the use phase. Similarly, if the recyclability of the aircraft is studied at the source, that is to say in its design, this will help to reduce the use of non-recyclable materials and dangerous compound. The greatest difficulty for recycling aircraft skeletons is that different materials are mixed and attached to each other. Through a thermodynamic study we showed what concentrations of alloying elements in a molten bath could be reduced. By using the Gibbs free energy, we evaluated some of the reactions taking place in a molten bath of aluminum during the injection of oxygen, boron, and chlorine. We focused on the reactions forming dual elements compounds, such as lithium oxide or magnesium chloride. We have shown that the six elements able to react when these reactions occurs are the lithium, the magnesium, the nickel, the titanium, the vanadium and the zirconium. It is necessary to remember that the impurities for alloys used in the aerospace industry are lower and especially those

  20. Evaluation of reciprocal differences in Bos indicus x Bos taurus backcross calves produced through embryo transfer: II. Postweaning, carcass, and meat traits.

    Science.gov (United States)

    Amen, T S; Herring, A D; Sanders, J O; Gill, C A

    2007-02-01

    Angus (A) x Bos indicus (B; Brahman or Nellore) reciprocal backcross, embryo transfer calves belonging to 28 full-sib families were evaluated for differences in feedyard initial BW, feedyard final BW, carcass weight, LM area, adjusted fat thickness, intramuscular fat, and Warner-Bratzler shear force. Two methods of analysis were investigated; method I made no distinction between how the F(1) parents were produced, whereas method II distinguished the 2 types of F(1) parents (AB vs. BA, corresponding to A x B vs. B x A, respectively). No significant reciprocal differences for these weight and carcass traits were detected under method I analyses, although the same trend existed for subsequent BW rankings as for birth weight and weaning weight. For each weight phase, the cross that involved a larger proportion of B in the sire in relation to the amount in the dam (F(1) x A and B x F(1)) ranked heavier than the respective reciprocal cross (A x F(1) and F(1) x B). As a whole, A backcross calves had larger (P carcass (P < 0.001), and had larger LM area (P < 0.05) with less adjusted fat (P < 0.001). No difference existed between the sexes for Warner-Bratzler shear force or marbling. No interactions involving sex, sire type, and dam type were observed for any of these traits. The results were similar under methods I and II analyses, with the exception that a significant sire type x dam type interaction was observed for initial feedyard BW. Results from this study suggest that for weight-related traits, both the breed constitution of the embryo transfer calf and the cross that produces the calf play an important role in its ultimate performance for B crossbred calves. For body composition and meat-related traits, it appears that the breed makeup of the embryo transfer calf itself is more important to animal performance than the specific cross used to produce the calf.

  1. EVALUATION OF MICROBIOLOGICAL AIR QUALITY INSIDE REFRIGERATION CELLS IN "AOSTA" MECHANIZED BRIGADE’S DEPENDENT REGIMENTS LOCATED IN EASTERN SICILY

    Directory of Open Access Journals (Sweden)

    A. Longo

    2011-01-01

    Full Text Available Authors evaluated the microbiological quality of air inside some refrigeration cells of the “AOSTA” Mechanized Brigade Regiments’ messes in Eastern Sicily, through an active air sampling and monitoring system. They used PCA medium plates for evaluation of microbial mesophilic amounts and Malt agar medium plates for the evaluation of mycotic flora. Study results showed values of TMC within a range of 25 – 273 CFU/m3 and values of mycotic concentrations within range of 10-1720 CFU/m3. All identified mycotic species belonged to Aspergillus and Penicillium genus. Furthermore, authors tested a duster, used for routine cleaning of all examined rooms, as it certainly represented a very important contamination means.

  2. [Physico-chemical and microbiological evaluation of UHT milk commercialized in three Mercosul countries (Brazil, Argentina and Paraguay)].

    Science.gov (United States)

    Domareski, Jackson Luiz; Bandiera, Nataly Simões; Sato, Rafael Tamostu; Aragon-Alegro, Lina Casale; de Santana, Elsa Helena Walter

    2010-09-01

    With the aim to evaluate the physico-chemical and microbiological quality of UHT milk commercialized in three countries of Mercosul, samples of four different brands were acquired in each city (Foz do Iguaçu-Brazil, Puerto Iguazú-Argentina and Ciudad del Este-Paraguay) and submitted to the following analysis: fat content, titratable acidity, milk ethanol stability (with the following ethanol concentrations: 68, 72, 76 and 80%), total dry extract and no fat dry extract, pH, density and freezing point. Counts of mesophilic and psychrotrophic microorganisms were already done. In the physico-chemical evaluation of UHT milk, a significant number of samples were in disagree with the established patterns for fat content, no fat dry extract, density and freezing point. Except one brand from Brazil, milk samples showed stability to 68% ethanol. pH averages of Brazilian milk were in agree with the patterns and highest values were observed in samples acquired on Paraguay. Observing the microbiological analysis, 37.5%, 62.5% and 12.5% of samples acquired from Brazil, Argentina and Paraguay, respectively, showed counts above the established patterns for mesophilic microorganisms. Counts of psychrotrophic microorganisms were in disagree with the established patterns in 50%, 50% and 100% of samples from Brazil, Argentina and Paraguay, respectively.

  3. Microbiological evaluation of hot beverages dispensed by vending machines from the Army barracks of Brigata Meccanizzata Aosta located in Messina

    Directory of Open Access Journals (Sweden)

    Chiara Beninati

    2013-04-01

    Full Text Available The aim of the present study was to evaluate the microbiological quality of hot beverages dispensed by vending machines (VMs. The study was carried out on 203 samples from 15 VMs located in 5 Army barracks in Messina. The samples included: water used for preparation of beverages, swab of water tank, swab of blender machine, chocolate powder, milk powder, cappuccino and chocolate drink (29 samples for each types. All samples were examined for total bacterial count (TBC, coliforms, Escherichia coli, enterococci, Pseudomonas aeruginosa, Staphylococcus aureus, Clostri - dium perfringens, Aeromonas spp., Salmonella spp. and Listeria monocytogenes. For the water samples the colony count (CC at 22°C and at 37°C was made. The average values of CC at 22°C and at 37°C were of 10.86x10²±8.72x10² CFU/mL and of 21.72x10²±16.44x10² CFU/mL, respectively. P. aeruginosa, coliform bacteria, S. aureus, E. coli and molds were detected from water. The TBC ranged from 176 CFU/g (±275.2 for chocolate powder to 294.8±69.4 CFU/g for milk powder. S. aureus and molds were isolated from milk powder, while coliforms, E. coli and S. aureus were observed in chocolate powder. The average TBC for hot beverages ranged from 34.32x10³±97.77x10³ CFU/mL for cappuccino to 36.59x10³±10.47x104 CFU/mL for chocolate drink. Coliforms, E. coli, enterococci and molds were detected from cappuccino, while enterococci and molds were observed in chocolate drink. The microbiological characteristics of the water and powders, hygiene, and the periodic cleaning of machines, influenced the microbiological quality of the hot beverages dispensed by VMs.

  4. Effects of chlorine or chlorine dioxide during immersion chilling on recovery of bacteria from broiler carcasses and chiller water

    Science.gov (United States)

    A study was conducted to determine the microbiological impact of immersion chilling broiler carcasses with chlorine or chlorine dioxide. Eviscerated, pre-chill commercial broiler carcasses were cut into left and right halves along the keel bone, and each half was rinsed (HCR) in 100 mL of 0.1% pept...

  5. Evaluation of E. coli biofilm as a protective barrier against microbiologically influenced deterioration of concrete (MICD) under mesophilic temperatures.

    Science.gov (United States)

    Soleimani, S; Ormeci, B; Isgor, O B

    2013-01-01

    In this study, Escherichia coli DH5α biofilm was evaluated for its potential to control and minimize microbiologically influenced concrete deterioration (MICD) under mesophilic temperatures (37 °C). Escherichia coli DH5α biofilm was first grown on Portland cement mortar disks for 8 days. Mortar disks were then exposed to two different types of sulfur oxidizing bacteria (SOB) (Thiobacillus neapolitanus and Thiobacillus thiooxidans), which use sulfur compounds as substrate and oxidize them to sulfate and sulfuric acid. The effectiveness of the biofilm against MICD was evaluated by measuring pH, sulfate, calcium concentrations in the reactors and surface analysis of the mortar samples using X-ray diffraction and visual inspection. Overall, the results indicate that the E. coli DH5α biofilm showed good protection against MICD induced by SOB at 37 °C.

  6. Microbiological assessment and evaluation of rehydration instructions on powdered infant formulas, follow-up formulas, and infant foods in Malaysia.

    Science.gov (United States)

    Abdullah Sani, N; Hartantyo, S H P; Forsythe, S J

    2013-01-01

    A total of 90 samples comprising powdered infant formulas (n=51), follow-up formulas (n=21), and infant foods (n=18) from 15 domestic and imported brands were purchased from various retailers in Klang Valley, Malaysia and evaluated in terms of microbiological quality and the similarity of rehydration instructions on the product label to guidelines set by the World Health Organization. Microbiological analysis included the determination of aerobic plate count (APC) and the presence of Enterobacteriaceae and Cronobacter spp. Isolates of interest were identified using ID 32E (bioMérieux France, Craponne, France). In this study, 87% of powdered infant formulas, follow-up formulas, and infant foods analyzed had an APC below the permitted level of 70°C for formula preparation, as specified by the 2008 revised World Health Organization guidelines. Six brands instructed the use of water at 40 to 55°C, a temperature range that would support the survival and even growth of Enterobacteriaceae. PMID:23141821

  7. Chemical Properties, Microbiological Quality and Sensory Evaluation of Chicken and Duck Liver Paste (foie gras

    Directory of Open Access Journals (Sweden)

    Abu-Salem, Ferial M.

    2010-06-01

    Full Text Available Liver paste or foie gras, which is a French term meaning “fatty liver”, was produced traditionally from goose and duck. Chickens are also used in the making of foie gras. The present study deals with the properties and quality of raw chicken and duck liver in comparison with manufactured liver paste (foie gras. Raw chicken liver contained 24.60% protein, 6.00% fat, 1.40 % ash, and 66.80% moisture. The average mineral values were 83.65, 50.75, 5.29, 1.15, 0.154, 0.683, 0.317 and 0.066 μg/g of Fe, Zn, Cu, Mn, Cd, Pb, Ni, and Cr, respectively. The processing of liver paste (Foie gras changed the composition of raw liver due to a loss in moisture, a release of fat and the addition of butter as a fat source. Chicken liver paste contained 27.8% moisture, 10.1% protein, 58.2% fat, and 0.8% ash. Mineral contents were 68.90, 40.50, 1.60, 1.1, 0.08, 0.22, 0.04 and 0.04 μg/g of Fe, Zn, Cu, Mn, Cd, Pb, Ni, and Cr, respectively. The chemical, microbiological and sensory evaluation of liver paste (foie gras manufactured from raw liver and preserved by the addition of 1000 ppm of both benzoic acid (BA or sorbic acid (SA and a mixture of 500 ppm of both BA plus SA with or without pasteurization at 85 °C were studied during the storage period for 9 days at 4 °C. Presumably, the mixing of liver paste (Foie gras from chicken liver with 500 ppm of both BA plus SA and pasteurizing the product at 85 °C could be recommended for lowering thiobarbituric acid (TBA, total volatile nitrogen (TVN, peroxide value (PV, free fatty acid (FFA, ammonia, saponification value and hence for inhibiting lipid oxidation and preventing rancidity to an extent up to nine days of refrigerated storage (4 °C. This level is also recommended as a preservative agent to inhibit the bacterial deterioration of chicken liver paste (Foie gras. A sensory evaluation showed that liver paste from chicken was very acceptable from the standpoint of taste, odor, appearance, color and texture. In

  8. Evaluating effects of sewage sludge and household compost on soil physical, chemical and microbiological properties

    DEFF Research Database (Denmark)

    Debosz, K.; Petersen, S.O.; Kure, L.K.;

    2002-01-01

    Recycling of organic wastes within agriculture may help maintain soil fertility via effects on physical, chemical and biological properties. Efficient use, however, requires an individual assessment of waste products, and effects should be compared with natural variations due to climate and soil......C, as well as in the field. The following properties were monitored: wet-stability of soil aggregates, clay dispersibility, hot-water extractable carbohydrates, resin-extractable P-i, inorganic N, biomass C and N, PLFA profiles, FDA hydrolysis activity, beta-glucosidase activity and CO2 evolution. In general...... amendment on the fraction of soil in wet-stable aggregates, or on the microbiological properties tested, which supported the observation from the incubation study that effects of organic wastes were transient. (C) 2002 Elsevier Science B.V. All rights reserved....

  9. Evaluation of commercial grated cheese in the central region of Brazil for microbiological quality and safety.

    Science.gov (United States)

    de Lima, Thaís Cristina Dias; da Silva, Patrícia Helena Caldeira; de Souza, Stefânia Márcia; Nero, Luís Augusto; Ferreira, Márcia de Aguiar

    2011-07-01

    This study aims to further the scientific understanding of the microbial quality and safety of grated cheese. Samples of grated cheese product (n = 20) were obtained from markets in the central region of Brazil and submitted to microbiological analysis using conventional and alternative (Petrifilm™ and RIDA(®) plates) methodologies. Based only on the criteria from the Brazilian Health Ministry, all samples were considered adequate for consumption. However, most samples presented foreign substances and high levels of contamination by other hygiene indicator microorganisms, indicating failures in processing and possible risks to consumers. Despite the hygienic quality of the samples, the obtained results showed good correlation indexes and similarities between the conventional and the alternative methodologies, indicating their viability for the quality control of grated cheese.

  10. Risk based microbiological criteria for Campylobacter in broiler meat in the European Union

    DEFF Research Database (Denmark)

    Nauta, Maarten; Sanaa, Moez; Havelaar, Arie H.

    2012-01-01

    Quantitative microbiological risk assessment (QMRA) allows evaluating the public health impact of food safety targets to support the control of foodborne pathogens. We estimate the risk reduction of setting microbiological criteria (MCs) for Campylobacter on broiler meat in 25 European countries......, applying quantitative data from the 2008 EU baseline survey. We demonstrate that risk based MCs can be derived without explicit consideration of Food Safety Objectives or Performance Objectives. Published QMRA models for the consumer phase and dose response provide a relation between Campylobacter...... analyses show that the estimated percentage of batches not complying with the MC is better correlated with the risk estimate than surrogate risk measures like the flock prevalence or the arithmetic mean concentration of bacteria on carcasses, and would therefore be a good measure for the risk...

  11. Prevalence and serogroup diversity of Salmonella for broiler neck skin, whole carcass rinse, and whole carcass enrichment sampling methodologies following air or immersion chilling

    Science.gov (United States)

    The purpose of this study was to evaluate neck skin (NS), whole carcass rinse (WCR), and whole carcass enrichment (WCE) sampling procedures for Salmonella isolation and serogroup from the same broiler carcass following either air or immersion chilling. Commercially processed and eviscerated broiler ...

  12. Evaluation of carcass characteristics and meat chemical composition of Bos indicus and Bos indicus x Bos taurus crossbred steers finished in pasture systems

    Directory of Open Access Journals (Sweden)

    Fernanda Barros Moreira

    2003-12-01

    Full Text Available The objective of this study was to evaluate the carcass characteristics (carcass weight, carcass yield, fat thickness, loin area, marbling and colour and chemical composition of the Longissimus dorsi muscle (moisture, ash, crude protein, fat and cholesterol of cuts with or without fat thickness, of Bos indicus and Bos indicus x Bos taurus crossbred steers finished in millet (Pennisetum americanum L. or star grass (Cynodon plectostachyus Pilger pasture systems, with mineral or mineral protein supplementation. Animals were slaughtered with an average body weight of 450 kg (Bos indicus or 470 kg (Bos indicus x Bos taurus crossbreed. There was no treatments effect on carcass characteristics and meat chemical composition of cut without fat thickness. The cuts with fat thickness of steers fed millet presented the highest fat deposition. Bos indicus steers presented higher carcass yield (57.23% and fat thickness (4.88 mm compared with crossbreed (53.40% and 3.05 mm. There was no breed effect on chemical composition of meat. The mean levels of cholesterol concentration were 31.41 mg/100 and 37.55 mg/100g of meat with and without fat thickness.O objetivo deste trabalho foi avaliar as características de carcaça (peso de carcaça, rendimento de carcaça, espessura de gordura de cobertura, área de olho de lombo, marmoreio e coloração e composição química do músculo Longissimus dorsi (umidade, cinzas, proteína bruta, gordura e colesterol de cortes, com ou sem gordura de cobertura, de novilhos Bos indicus e mestiços Bos indicus x Bos taurus terminados em sistemas em pastagem de milheto (Pennisetum americanum L. ou grama estrela (Cynodon plectostachyus Pilger, com suplementação mineral ou protéica e mineral. Os animais foram abatidos com peso médio de 450 kg (Bos indicus ou 470 kg (mestiços. Não houve efeito de tratamento sobre as características de carcaça e composição da carne com ou sem gordura de cobertura. Os cortes com gordura de

  13. Microbiological, nutritional and sensory evaluation of long-time stored amaranth biscuits produced from irradiated-treated amaranth grains

    International Nuclear Information System (INIS)

    The paper presents some results achieved by the evaluation of microbiological (total bacterial count, coliform bacteria, aerobic sporeforming bacteria, yeasts and moulds( nutritional (lysine) and sensory (shape, surface, colour consistency, taste, odour, the profiling of tastiness) quality and of the aw values of amaranth-based biscuits produced from the amaranth grain irradiated by various ionizing radiation doses (1.5, 3 and 5 kGy, source 60Co) and stored for the period of 12 months at the laboratory temperature (20–25°C). The irradiation dose providing the biscuits maximum hygienic, nutritional and sensory quality maintained up to the end of the one-year storage was 5 kGy

  14. Evaluation of the ionizing radiation effects in microbiology, physical and chemical and sensory aspects of ice cream

    International Nuclear Information System (INIS)

    The ice cream is defined as an emulsion of fats and proteins or a mixture of water and sugar, other ingredients may be added provided since they do not affect the product. It is considered a food of high nutritional value, providing lipids, carbohydrates, protein, calcium, phosphorus, and other minerals and vitamins (A, B1, B2, B6, C, D, E and K), and it is considered one of the most important products and higher interest to the dairy industry due to great demand by the consumers. The diseases related to food consumption are considered one of the most significant problems. Several outbreaks related to microbiological contamination of ice cream have been reported in recent decades in Asia, Europe and America. It is believed that the ice cream, as a frozen food, presents no risk to the population health. However, it is considered an excellent environment for the growth of microorganisms due to its composition, pH close to neutrality and long storage period. The aim of this study was to evaluate the microbiological, sensory and physicochemical aspects of ice cream. The ice cream samples were irradiated with gamma rays (60Co) with the doses of LOkGy, 2.0kGy, 3.0kGy and 4.0kGy. The samples intended for the inoculation of Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 11229) and Salmonella abaetetuba (ATCC 35640) have been irradiated with doses of 1.0, 2.0 and 3.0kGy. It can be concluded that the dose of 3.0kGy was adequate to reduce most of the studied microorganisms to undetected levels. The use of gamma radiation affected the texture and the parameters of the colorimetric analyses of the ice cream. The results of the sensorial analyses showed that the better accepted dose was 3.0kGy. (author)

  15. Microbiological evaluation of water during the 2011 flood crisis in Thailand.

    Science.gov (United States)

    Chaturongkasumrit, Yuphakhun; Techaruvichit, Punnida; Takahashi, Hajime; Kimura, Bon; Keeratipibul, Suwimon

    2013-10-01

    In 2011, a severe flood occurred in Thailand, covering nearly half the country in water for several months. The contamination of floodwater and subsequent contamination of water for human consumption could have potentially led to a widespread health crisis. However, to date, no study has been conducted to determine the safety of the waters used for human consumption in Thailand during the severe flood. Therefore, we conducted microbiological analysis of 4 kinds of water (floodwater, river water, tap water, and filtered tap water) collected from industrial and residential areas that were damaged due to flooding. Higher net levels of bacteria were found in water with a higher turbidity. No clear trend was observed in the pH value of all 4 water samples. The level of total bacterial contamination in the water samples was estimated by real-time quantitative polymerase chain reaction (PCR). Eleven of the 12 tap water samples and all of the filtered tap water samples had a total bacterial load that exceeded the Thai water quality standards. One of the tap water samples and one of the filtered tap water samples were found to be positive for Shigella sp., although none of the floodwater samples showed detectable levels of this pathogen as determined by PCR analysis. One of the samples of floodwater was also found to be positive for Leptospira sp., but none of the tap water or filtered tap water samples were positive. Most of the tap water samples and all filtered tap water samples were found to be contaminated with Vibrio cholerae. Bacterial contamination in water samples was also analyzed by denaturing gradient gel electrophoresis (DGGE) analysis. These results revealed that several microorganisms were transferred via floodwater to different areas in the central part of Thailand and cross-contaminated between floodwater and water for human consumption.

  16. Coral microbiology

    Science.gov (United States)

    Rosenberg, Eugene; Kellogg, Christina A.; Rohwer, Forest

    2007-01-01

    In the last 30 years, there has been approximately a 30% loss of corals worldwide, largely due to emerging diseases (Harvell et al., 2002, 2007; Hughes et al., 2003). Coral microbiology is a new field, driven largely by a desire to understand the interactions between corals and their symbiotic microorganisms and to use this knowledge to eventually prevent the spread of coral diseases.

  17. Evaluation of reciprocal differences in Bos indicus x Bos taurus backcross calves produced through embryo transfer: II. Postweaning, carcass, and meat traits.

    Science.gov (United States)

    Amen, T S; Herring, A D; Sanders, J O; Gill, C A

    2007-02-01

    Angus (A) x Bos indicus (B; Brahman or Nellore) reciprocal backcross, embryo transfer calves belonging to 28 full-sib families were evaluated for differences in feedyard initial BW, feedyard final BW, carcass weight, LM area, adjusted fat thickness, intramuscular fat, and Warner-Bratzler shear force. Two methods of analysis were investigated; method I made no distinction between how the F(1) parents were produced, whereas method II distinguished the 2 types of F(1) parents (AB vs. BA, corresponding to A x B vs. B x A, respectively). No significant reciprocal differences for these weight and carcass traits were detected under method I analyses, although the same trend existed for subsequent BW rankings as for birth weight and weaning weight. For each weight phase, the cross that involved a larger proportion of B in the sire in relation to the amount in the dam (F(1) x A and B x F(1)) ranked heavier than the respective reciprocal cross (A x F(1) and F(1) x B). As a whole, A backcross calves had larger (P x dam type interaction was observed for initial feedyard BW. Results from this study suggest that for weight-related traits, both the breed constitution of the embryo transfer calf and the cross that produces the calf play an important role in its ultimate performance for B crossbred calves. For body composition and meat-related traits, it appears that the breed makeup of the embryo transfer calf itself is more important to animal performance than the specific cross used to produce the calf. PMID:17235022

  18. Yield of warm beef carcass meat from simmental breed

    OpenAIRE

    Kuzelov, Aco

    2010-01-01

    The objective of this study was to evaluate the influence of live weight upon yield of warm beef carcass meat from Simmental breed. Twenty 12 - 18 month-old male bullocks were assigned with average live weight 450,52 ± 4,68 kg. The yield of warm beef carcass meat, edible and non-edible by-products was determined

  19. Microbiological evaluation of water during the 2011 flood crisis in Thailand

    Energy Technology Data Exchange (ETDEWEB)

    Chaturongkasumrit, Yuphakhun; Techaruvichit, Punnida; Takahashi, Hajime; Kimura, Bon [Department of Food Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology, Tokyo 108-8477 (Japan); Keeratipibul, Suwimon, E-mail: Suwimon.k@chula.ac.th [Department of Food Technology, Faculty of Science, Chulalongkorn University, Phayathai Road, Bangkok 10330 (Thailand)

    2013-10-01

    In 2011, a severe flood occurred in Thailand, covering nearly half the country in water for several months. The contamination of floodwater and subsequent contamination of water for human consumption could have potentially led to a widespread health crisis. However, to date, no study has been conducted to determine the safety of the waters used for human consumption in Thailand during the severe flood. Therefore, we conducted microbiological analysis of 4 kinds of water (floodwater, river water, tap water, and filtered tap water) collected from industrial and residential areas that were damaged due to flooding. Higher net levels of bacteria were found in water with a higher turbidity. No clear trend was observed in the pH value of all 4 water samples. The level of total bacterial contamination in the water samples was estimated by real-time quantitative polymerase chain reaction (PCR). Eleven of the 12 tap water samples and all of the filtered tap water samples had a total bacterial load that exceeded the Thai water quality standards. One of the tap water samples and one of the filtered tap water samples were found to be positive for Shigella sp., although none of the floodwater samples showed detectable levels of this pathogen as determined by PCR analysis. One of the samples of floodwater was also found to be positive for Leptospira sp., but none of the tap water or filtered tap water samples were positive. Most of the tap water samples and all filtered tap water samples were found to be contaminated with Vibrio cholerae. Bacterial contamination in water samples was also analyzed by denaturing gradient gel electrophoresis (DGGE) analysis. These results revealed that several microorganisms were transferred via floodwater to different areas in the central part of Thailand and cross-contaminated between floodwater and water for human consumption. - Highlights: • We investigated the flood impact on the waters used for human consumption. • Higher net levels of

  20. Effects of Rice Bran, Flax Seed, and Sunflower Seed on Growth Performance, Carcass Characteristics, Fatty Acid Composition, Free Amino Acid and Peptide Contents, and Sensory Evaluations of Native Korean Cattle (Hanwoo)

    OpenAIRE

    Choi, Chang Bon; Kwon, Hana; Kim, Sung Il; Yang, Un Mok; Lee, Ju Hwan; Park, Eun Kyu

    2016-01-01

    This study was conducted to evaluate the effect of dietary supplementation with rice bran, flax seed, or sunflower seed to finishing native Korean cattle (Hanwoo) on growth performances, carcass characteristics, fatty acid composition, free amino acid and peptide contents, and sensory evaluations of Longissimus muscle (LM). A total of 39 Hanwoo steers (average age of 22.2 mo and average body weight (BW) of 552.2 kg) were randomly divided into Control, rice bran (RB), flax seed (FS), or Sunflo...

  1. Comparative evaluation of subgingival plaque microflora in pregnant and non-pregnant women: A clinical and microbiologic study

    Directory of Open Access Journals (Sweden)

    Rishi Emmatty

    2013-01-01

    Full Text Available Context: Gingival changes in pregnancy have been attributed to changes in the subgingival biofilm related to hormonal variations. Aims: To evaluate the subgingival plaque microflora in pregnant and nonpregnant women to determine if pregnancy induces any alterations in the subgingival plaque and to associate these changes with changes in periodontal status. Settings and Design: Thirty pregnant and 10 nonpregnant women within the age group of 20-35 years having a probing pocket depth (PPD of 3-4 mm were included in the study. The pregnant women were equally divided into 3 groups of 10, each belonging to I, II, and III trimester. Materials and Methods: Plaque index, gingival index, PPD, and microbiologic evaluation for specific bacterial counts for Prevotella intermedia, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Parvimonas micra, and Fusobacterium nucleatum were carried out for all subjects. Statistical Analysis: Mann-Whitney U test. Results: Increase in gingival inflammation was observed in II and III trimester as compared with I trimester and control. Plaque scores did not show any significant difference between pregnant and nonpregnant women. Specific bacterial examination revealed an increase in proportion of P. intermedia in pregnant women of both II and III trimester as compared with I trimester and nonpregnant women. Conclusions: A definite increase in proportions of P. intermedia occurs in subgingival plaque microflora in pregnancy that may be responsible for the exaggerated gingival response.

  2. Microbial Biofilm and Bacterial Contamination on Pig Carcasses

    Directory of Open Access Journals (Sweden)

    Adriana Morar

    2010-10-01

    Full Text Available The aim of this study was to emphasize the presence of biofilm on meat surfaces using epifluorescences microscopy and establishing the microbial contamination level by classical microbiological methods. The research was performed in a pork slaughterhouse. The presence of microbial biofilm and the level of contamination were performed on surfaces from pig carcasses and cut pieces. Clusters of microorganisms included in a biofilm matrix were found on the surface of carcasses on sternal region, coast region, coccigian region and on surfaces of cut pieces: chop, front of thighs. Microbial biofilm was present on carcasses and cut pieces at least 3 days length, in regions with high humidity and microbial contamination level ranged of 102- 103 cfu/ cm2. The microbial load of the surfaces was assessed using the following microbiological indicators: total viable count (TVC, the number of enterobacteria and Pseudomonas genus. The level of carcasses contamination ranged on average from 1.3 x 10 cfu/ cm2 (neck to 2.6 x 103 cfu/cm2 (front of pulp. The proportion of Enterobacteriaceae-positive samples was 60%, with a low level of contamination (less than 1 cfu/ cm2. Germs of the Pseudomonas genus were absent in all the analyzed samples.

  3. The effect of crossing of domestic Simmental breed and French fattening breeds on conformation and fat cover of beef carcasses

    OpenAIRE

    Ostojić-Andrić D.; Aleksić S.; Petrović M.M.; Hristov S.; Pantelić V.; Novaković Ž.; Nikšić D.

    2011-01-01

    Evaluation of conformation and fat cover of beef carcasses has great importance in modern systems of carcass quality evaluation. In this way, adequate price is achieved for every classified carcass side according to market demand. In this paper, the effect of experimental crossing of Domestic Simmental breed with Charolais and Limousine breed (N=96) on conformation and fat cover was investigated. Evaluation was carried out on the whole carcass and partially...

  4. Phisicochemical, sensory, and microbiological evaluation and development of symbiotic fermented drink

    Directory of Open Access Journals (Sweden)

    Mônica de Lucena Lira Aguiar Dias

    2013-12-01

    Full Text Available The goal of this study was to develop a symbiotic lacteous drink, evaluate its physicochemical and sensory characteristics, and verify the viability of Lactobacillus acidophilus in the drink. The milk serum-based drink consisted of 50% milk serum containing 10% saccharose, 25% powdered milk, 15% yacon pulp, and cultures of Lactobacillus acidophilus-La 5E and Bifidobacterium bifidum BB12. It was stored for up to 21 days under refrigeration. The milk serum-based drink was analyzed for protein, ether extract, total dietary fiber, total frutans, carbohydrate content, color, pH, acidity, and contamination by coliforms, and Salmonella sp. Coliforms and Salmonella sp were not detected, and L. acidophilus and B. bifidum provided satisfactory probiotic counts up to 21 days of storage under refrigeration. Lactobacillus acidophilus resistance to gastric acids and bile salts was detected only up to seven days of storage when evaluated in vitro. Sensory analysis and purchase viability were evaluated by consumers at 0, 7, 14, and 21 days of storage. Based on the analytical results and consumer evaluation, the drink was acceptable up to seven days of storage.

  5. Microbiological and Pharmacological Evaluation of the Micropropagated Rubus liebmannii Medicinal Plant

    Science.gov (United States)

    Jiménez-Arellanes, Adelina; Cornejo-Garrido, Jorge; Rojas-Bribiesca, Gabriela; Nicasio-Torres, María del Pilar; Said-Fernández, Salvador; Mata-Cárdenas, Benito David; Molina-Salinas, Gloria María; Tortoriello, Jaime; Meckes-Fischer, Mariana

    2012-01-01

    Rubus liebmannii is an endemic species from Mexico used in traditional medicine primarily to treat dysentery and cough. The in vitro activity against Giardia lamblia and Entamoeba histolytica that produces the ethanolic extract of the aerial parts of the plant led us to expand the pharmacological and phytochemical research of this species. Gastrointestinal disorders including amebiasis remain one of the health problems that need to be addressed and it is of interest to find alternatives that improve their treatment. Also, it is important to emphasize that R. liebmannii grows wild in the country and is not found in abundance; therefore, alternatives that avoid overexploitation of the natural resource are mandatory. Ongoing with the evaluation of the potentialities that R. liebmannii possesses for treating infectious gastrointestinal diseases, the aim of the present study was to evaluate the biological effects and the chemical composition of the micropropagated plant. PMID:22966243

  6. Microbiological and Pharmacological Evaluation of the Micropropagated Rubus liebmannii Medicinal Plant

    Directory of Open Access Journals (Sweden)

    Adelina Jiménez-Arellanes

    2012-01-01

    Full Text Available Rubus liebmannii is an endemic species from Mexico used in traditional medicine primarily to treat dysentery and cough. The in vitro activity against Giardia lamblia and Entamoeba histolytica that produces the ethanolic extract of the aerial parts of the plant led us to expand the pharmacological and phytochemical research of this species. Gastrointestinal disorders including amebiasis remain one of the health problems that need to be addressed and it is of interest to find alternatives that improve their treatment. Also, it is important to emphasize that R. liebmannii grows wild in the country and is not found in abundance; therefore, alternatives that avoid overexploitation of the natural resource are mandatory. Ongoing with the evaluation of the potentialities that R. liebmannii possesses for treating infectious gastrointestinal diseases, the aim of the present study was to evaluate the biological effects and the chemical composition of the micropropagated plant.

  7. Phisicochemical, sensory, and microbiological evaluation and development of symbiotic fermented drink

    OpenAIRE

    Mônica de Lucena Lira Aguiar Dias; Silvana Magalhães Salgado; Nonete Barbosa Guerra; Alda Verônica de Souza Livera; Samara Alvachian Cardoso Andrade; Graciliane Nobre da Cruz Ximenes

    2013-01-01

    The goal of this study was to develop a symbiotic lacteous drink, evaluate its physicochemical and sensory characteristics, and verify the viability of Lactobacillus acidophilus in the drink. The milk serum-based drink consisted of 50% milk serum containing 10% saccharose, 25% powdered milk, 15% yacon pulp, and cultures of Lactobacillus acidophilus-La 5E and Bifidobacterium bifidum BB12. It was stored for up to 21 days under refrigeration. The milk serum-based drink was analyzed for protein, ...

  8. Evaluation of biogenic amines levels, and biochemical and microbiological characterization of Italian-type salami sold in Rio de Janeiro, Brazil

    OpenAIRE

    Luiz Felipe Lopes dos Santos; Eliane Teixeira Mársico; César Aquiles Lázaro; Rose Teixeira; Laís Doro; Carlos Adam Conte Júnior

    2015-01-01

    The objective of the present study was to evaluate the levels of biogenic amines (cadaverine, putrescine, tyramine, histamine, spermidine and spermine) by high performance liquid chromatography (HPLC) and the physicochemical (moisture, lipids, proteins, pH, water activity and fixed mineral residue) and microbiological (lactic acid bacteria and aerobic heterotrophic mesophilic bacteria count) characteristics of six Italian-type salami brands sold in the city of Niteroi (Rio de Janeiro, Brazil)...

  9. EVALUATION OF THE CORRELATION BETWEEN THE INFECTION STATUS OF UDDER AND THE MICROBIOLOGICAL MILK QUALITY IN SOME EXTENSIVE GOAT HERDS OF SARDINIA - PRELIMINARY RESULTS

    OpenAIRE

    M. Mulas; G. Bassu; A. Coccollone; G. Masia; C. Muzzigoni; M. Picoi; P.A. Cabras

    2011-01-01

    Sub clinical mastitis may cause more losses than clinical mastitis. Farmers can take advantage of employing several tools, California Mastitis Test (CMT) and Somatic Cell Count (SCC) among the others, to determine the presence of a sub clinical mastitis in their herds. The aim of the present study was to evaluate the microbiological quality of milk produced in some extensive goat herds in Sardinia through a clinical check to determine the infection status of the udder, CMT, SCC and microbiolo...

  10. A SYSTEMATIC APPROACH TO THE EVALUATION OF POST G RADUATE PRACTICAL EXAMINATIONS IN MEDICAL MICROBIOLOGY - AN OVERVIEW

    Directory of Open Access Journals (Sweden)

    Prathab

    2012-10-01

    Full Text Available ABSTRACT: BACKGROUND: Assessment forms an essential part of any curriculum . There are several reasons why we need to assess student perfor mance, most important of which is that it forms a basis for certifying student-doctors as Hea lth-care professionals who practice in society, It also needs to be realized that assessment drives learning and therefore all the desired outcomes need to be taken into account while assess ing .A well formatted blueprint will go a long way in fulfilling these objectives (1, 2. The entire process of evaluation of post graduate pra ctical examinations in Medical microbiology is done over a period of three days. Th e students have to perform a number of exercises with the end result of the tasks performed on a particular day yielding results on the next or the last day. Therefore it is not easy to m aintain objectivity in assessment unless we have a concrete system of evaluation in place. Hence, there was a felt need to prepare and try out a blueprint for examining post graduates in practical examinations which would brin g about the desired outcomes in terms of maximum objectivity and testing of each individual component of the desired skill tested for. The scheme when adopted for use in exams was found t o yield satisfactory results in comparison to conventional methods of practice in eva luation. This may also be used as a guide to plan and prepare s imilar documents according to the Syllabi and Guidelines followed by different univer sities in the subject specialty mentioned here or even for other subjects or courses .

  11. Performance, carcass traits and economic evaluation of the replacement of corn whole grain by pelleted soybean hulls in the feeding of feedlot lambs

    Directory of Open Access Journals (Sweden)

    Tiago Galafassi Zarpelon

    2015-04-01

    Full Text Available This work was carried out with the objective of evaluating the effects of different levels of replacement of whole grain corn by pelleted soybean hulls in high concentrated diet, without long fiber use, on intake, productive performance and economical viability of feedlot lambs production. Twenty four Texel lambs, 12 males and 12 females of the same age, with average weight of 23 kg, distributed in 12 stalls, in a completely randomized design, were used. The treatments consisted of replacement of whole grain corn by pelleted soybean hulls, at levels of 0% (SH0, 15% (SH15 and 30% (SH30. Dry matter (DM (kg day-1, crude protein (CP (kg day-1, %AW, g AW0,75, and neutral detergent fiber (NDF (kg day-1, %AW, g AW0,75 intake showed linear increasing behavior (P<0.05 according to increasing levels of soybean hulls replacing the corn grain in the diet, however, the ether extract (EE intake (g day-1 presented quadratic effect. There were no differences in DM intake in relation to weight and metabolic body size, as well as, to mineral material (MM (g day-1 and total digestible nutrients (TDN intake (kg day-1, %AW, g AW0,75. In average daily gain (ADG, a quadratic effect of different levels of soybean hulls in the diet was observed (P<0.05, with weight gain of 0.267; 0.327 and 0.224 kg day-1, in levels of 0, 15 and 30%, respectively. The feed conversion was affected in a increasing linear way (P<0.05 with the soybean hulls inclusion in the diet. It was not observed differences in the carcass traits. When increasing levels of soybean hulls were added to the diet, it was observed decreasing in cost/kg ration (R$, however, there was increasing cost of meat produced (kg. In a general way, it can be concluded that replacement of whole corn by pelleted soybean hulls in high concentrated diet, in a proportion of 15%, increases the weight gain in feedlot lambs. In the proportion of 30%, affect negatively the animal performance, however does not influence the

  12. Studies on rheology and microbiological evaluation of hydrotropically gelled starch as topical vehicle for terbinafine hydrochloride

    Directory of Open Access Journals (Sweden)

    Rao Kovur

    2008-01-01

    Full Text Available The present study was aimed for the development and evaluation of hydrotropic starch gels of terbinafine hydrochloride which is an allylamine derivative of antifungal agent was formulated by using corn starch and sodium salicylate as hydrotropic salt. The gels were prepared in presence and absence of propylene glycol. The prepared gels were evaluated for in vitro drug release, rheological behavior and microbial studies. The degree of increase in the drug diffusion was found to be in order corn starch gel with propylene glycol was greater than corn starch gel without propylene glycol. The microbial studies were carried out in soya bean casein digest medium with Candida albicans as test organisms and were found to be 22.86 ± 0.58 and 20.22 ± 0.65 mm for TCSG (IV and TCS (III, respectively. All the gels exhibited shear thinning. The rheogram indicated that the gel systems are pseudoplastic and exhibited thixotropy. The added propylene glycol has not appreciably altered the apparent viscosity values.

  13. Short communication: Evaluation of bulk tank milk microbiological quality of nine dairy farms in Tennessee.

    Science.gov (United States)

    Gillespie, B E; Lewis, M J; Boonyayatra, S; Maxwell, M L; Saxton, A; Oliver, S P; Almeida, R A

    2012-08-01

    The purpose of this study was to evaluate the bulk tank milk (BTM) quality of 9 East Tennessee dairy farms and to determine its relationship with selected quality milk parameters. Bulk tank milk samples (n=1,141) were collected over a 42-mo period (June 2006 through November 2009) from farms, based on their preliminary incubation count (PIC) history. Parameters of BTM quality evaluated in this study included somatic cell count (SCC), standard plate count (SPC), PIC, laboratory pasteurization count (LPC), Staphylococcus spp. count, Streptococcus spp. count, and coliform count. Strong correlations between SPC and Streptococcus spp. counts (0.72) and between SPC and PIC (0.70) were found. However, moderate correlations were seen among other milk quality parameters. In addition, seasonal variations for some milk quality parameters were noted. For example, milk quality parameters such as SCC, SPC, LPC, and coliform count were significantly higher in summer, whereas Streptococcus spp. counts were significantly higher in winter. No seasonal variation in PIC or Staphylococcus spp. counts was observed. Summarizing, results from this investigation showed the importance of using several bacterial counts (SCC, SPC, PIC, LPC, Streptococcus spp. count, Staphylococcus spp. count, and coliform counts) as simultaneous indicators of milk quality.

  14. Field Evaluation of a Semiautomated Method for Rapid and Simple Analysis of Recreational Water Microbiological Quality

    Science.gov (United States)

    Anglès d'Auriac, Marc B.; Roberts, Hildegarde; Shaw, Terri; Sirevåg, Reidun; Hermansen, Leonila Fajardo; Berg, James D.

    2000-01-01

    An early warning system using a rapid enzymatic semiautomated method suitable for fecal coliform detection in recreational waters within 8 h was developed further and evaluated in this study. This rapid method was compared to the standard method followed in the United Kingdom. We used 1,011 samples originating from 206 different locations in Wales. When we assessed the presence or absence of fecal coliforms, targeting very low levels of contamination, we obtained 83.9% agreement between the rapid method and the lauryl sulfate broth-membrane filtration technique, whereas direct confirmation of the samples processed by the rapid method showed 89.3% agreement. Environmental enzymatic background activity was found to be the main limiting factor for this method. Owing to a specific and integrated handling of the results by the software of the instrument, the percentage of false-positive results (a consequence of enzymatic background) was successfully limited to 2.9% by the direct confirmation evaluation. However, 7.8% false-negative results due to “late-growers” had to be accepted in order to produce results within a working day. At present, the method can be used in a more conservative way to assess the environmental threshold of 100 CFU of fecal coliforms per 100 ml in recreational waters. The implications of our findings with regard to the applicability of rapid enzymatic methods are discussed. PMID:11010890

  15. The chilling of carcasses.

    Science.gov (United States)

    Savell, J W; Mueller, S L; Baird, B E

    2005-07-01

    Biochemical processes and structural changes that occur in muscle during the first 24h postmortem play a great role in the ultimate quality and palatability of meat and are influenced by the chilling processes that carcasses are subjected to after slaughter. For beef and lamb, employing chilling parameters that minimize cold shortening is of greatest importance and can be best addressed by ensuring that muscle temperatures are not below 10°C before pH reaches 6.2. For pork, because of the impact of high muscle temperatures and low pH on the development of pale, soft, and exudative (PSE) pork, a more rapid chilling process is needed to reduce PSE with the recommended internal muscle temperature of 10°C at 12h and 2-4°C at 24h. Spray chilling, a system whereby chilled water is applied to carcasses during the early part of postmortem cooling, is used to control carcass shrinkage and to improve chilling rates through evaporative cooling. Delayed chilling can be used to reduce or prevent the negative effects of cold shortening; however, production constraints in high-volume facilities and food safety concerns make this method less useful in commercial settings. Electrical stimulation and alternative carcass suspension programs offer processors the opportunity to negate most or all of the effects of cold shortening while still using traditional chilling systems. Rapid or blast chilling can be an effective method to reduce the incidence of PSE in pork but extreme chilling systems may cause quality problems because of the differential between the cold temperatures on the outside of the carcass compared to the warm muscle temperatures within the carcass (i.e., muscles that are darker in color externally and lighter in color internally).

  16. Microbiological evaluation of different strategies for management of snakes in captivity.

    Science.gov (United States)

    Campagner, M V; Bosco, S M G; Bagagli, E; Cunha, M L R S; Jeronimo, B C; Saad, E; Biscola, N P; Ferreira, R S; Barraviera, B

    2012-01-01

    Keeping snakes in captivity to produce venom for scientific research and production of inputs is now a worldwide practice. Maintaining snakes in captivity involves capture, infrastructure investments, management techniques, and appropriate qualified personnel. Further, the success of the project requires knowledge of habitat, nutrition, and reproduction, and control of opportunistic infections. This study evaluated the management of snakes in three types of captivity (quarantine, intensive, and semiextensive) and diagnosed bacterial and fungal contaminants. A bacteriological profile was obtained by swabbing the oral and cloacal cavities, scales, and venoms of healthy adult snakes from Bothrops jararaca (Bj) and Crotalus durissus terrificus (Cdt). There was predominance of Enterobacteriaceae, especially non-fermenting Gram-negative bacilli excluding Pseudomonas spp and Gram- positive bacteria. Statistically, intensive captivity resulted in the highest number of bacterial isolates, followed by recent capture (quarantine) and by semiextensive captivity. No statistical difference was found between Bj and Cdt bacterial frequency. In vitro bacterial susceptibility testing found the highest resistance against the semisynthetic penicillins (amoxicillin and ampicillin) and highest sensitivity to amicacin and tobramycin aminoglycosides. To evaluate mycological profile of snakes from intensive captivity, samples were obtained from two healthy Bj and one B. moojeni, one B. pauloensis, and one Cdt showing whitish lesions on the scales suggestive of ringworm. Using conventional methods and DNA-based molecular procedures, five samples of Trichosporon asahii were identified. Despite the traditional role of intense captivity in ophidian venom production, semiextensive captivity was more effective in the present study by virtue of presenting superior control of bacterial and fungal transmission, easier management, lowest cost, and decreased rate of mortality; therefore, it should be

  17. Preparation and Microbiological Evaluation of Amphiphilic Kanamycin-Lipoamino Acid Ion-Pairs

    Directory of Open Access Journals (Sweden)

    Rosario Pignatello

    2014-05-01

    Full Text Available Amphiphilic ion-pairs of kanamycin (KAN were prepared by evaporation of a water-ethanol co-solution of KAN base and a lipoamino acid bearing a 12-carbon atoms alkyl side chain (LAA12, at different molar ratios. Infrared spectroscopy confirmed the structure of ion-pairs, while differential scanning calorimetry (DSC and powder X-ray diffractometry (PXRD studies supported the formation of new saline species with a different crystalline structure than the starting components. The solubility pattern shown in a range of both aqueous and organic solvents confirmed that the ion-pairs possess an amphiphilic character. The LAA12 counter-ion showed not to improve the antibacterial activity of KAN, suggesting that such chemical strategy is not able to favor the penetration of this drug inside the bacteria cells. Nevertheless, a slight improving, i.e., a one-fold dilution, was observed in E. coli. The present study can also serve as the basis for a further evaluation of LAA ion-pairing of antibiotics, as a means to improve the loading of hydrophilic drugs into lipid-based nanocarriers.

  18. Evaluation of a commercially available organic acid product on body weight loss, carcass yield, and meat quality during preslaughter feed withdrawal in broiler chickens: a poultry welfare and economic perspective.

    Science.gov (United States)

    Menconi, A; Kuttappan, V A; Hernandez-Velasco, X; Urbano, T; Matté, F; Layton, S; Kallapura, G; Latorre, J; Morales, B E; Prado, O; Vicente, J L; Barton, J; Andreatti Filho, R L; Lovato, M; Hargis, B M; Tellez, G

    2014-02-01

    The effect of a commercial organic acid (OA) product on BW loss (BWL) during feed withdrawal and transportation, carcass yield, and meat quality was evaluated in broiler chickens. Two experiments were conducted in Brazil. Commercial houses were paired as control groups receiving regular water and treated groups receiving OA in the water. Treated birds had a reduction in BWL of 37 g in experiment 1 and 32.2 g in experiment 2. In experiment 2, no differences were observed in carcass yield between groups. Estimation of the cost benefit suggested a 1:16 ratio by using the OA. In experiment 3, conducted in Mexico, significant differences on water consumption, BWL, and meat quality characteristics were observed in chickens that were treated with the OA (P animal welfare and economic concerns in the poultry industry by reducing BWL and improving meat quality attributes.

  19. Evaluation of a commercially available organic acid product on body weight loss, carcass yield, and meat quality during preslaughter feed withdrawal in broiler chickens: a poultry welfare and economic perspective.

    Science.gov (United States)

    Menconi, A; Kuttappan, V A; Hernandez-Velasco, X; Urbano, T; Matté, F; Layton, S; Kallapura, G; Latorre, J; Morales, B E; Prado, O; Vicente, J L; Barton, J; Andreatti Filho, R L; Lovato, M; Hargis, B M; Tellez, G

    2014-02-01

    The effect of a commercial organic acid (OA) product on BW loss (BWL) during feed withdrawal and transportation, carcass yield, and meat quality was evaluated in broiler chickens. Two experiments were conducted in Brazil. Commercial houses were paired as control groups receiving regular water and treated groups receiving OA in the water. Treated birds had a reduction in BWL of 37 g in experiment 1 and 32.2 g in experiment 2. In experiment 2, no differences were observed in carcass yield between groups. Estimation of the cost benefit suggested a 1:16 ratio by using the OA. In experiment 3, conducted in Mexico, significant differences on water consumption, BWL, and meat quality characteristics were observed in chickens that were treated with the OA (P poultry industry by reducing BWL and improving meat quality attributes. PMID:24570468

  20. EVALUATION OF THE CORRELATION BETWEEN THE INFECTION STATUS OF UDDER AND THE MICROBIOLOGICAL MILK QUALITY IN SOME EXTENSIVE GOAT HERDS OF SARDINIA - PRELIMINARY RESULTS

    Directory of Open Access Journals (Sweden)

    M. Mulas

    2011-01-01

    Full Text Available Sub clinical mastitis may cause more losses than clinical mastitis. Farmers can take advantage of employing several tools, California Mastitis Test (CMT and Somatic Cell Count (SCC among the others, to determine the presence of a sub clinical mastitis in their herds. The aim of the present study was to evaluate the microbiological quality of milk produced in some extensive goat herds in Sardinia through a clinical check to determine the infection status of the udder, CMT, SCC and microbiological milk tests. CMT has been confirmed to be a useful, practical and economical tool to detect sub clinical mastitis in goats. Farmers should be encouraged to use this as a first step in order to avoid prospective losses in their herds.

  1. Evaluation of the microbiological quality of reclaimed water produced from a lagooning system.

    Science.gov (United States)

    Fernandez-Cassi, X; Silvera, C; Cervero-Aragó, S; Rusiñol, M; Latif-Eugeni, F; Bruguera-Casamada, C; Civit, S; Araujo, R M; Figueras, M J; Girones, R; Bofill-Mas, S

    2016-08-01

    The use of lagooning as a complementary natural method of treating secondary effluents of wastewater treatment plants has been employed as an affordable and easy means of producing reclaimed water. However, using reclaimed water for some purposes, for example, for food irrigation, presents some risks if the effluents contain microbial pathogens. Classical bacterial indicators that are used to assess faecal contamination in water do not always properly indicate the presence of bacterial or viral pathogens. In the current study, the presence of faecal indicator bacteria (FIB), heterotrophic bacterial counts (HBC), pathogens and opportunistic pathogens, such as Legionella spp., Aeromonas spp., Arcobacter spp., free-living amoeba (FLA), several viral indicators (human adenovirus and polyomavirus JC) and viral pathogens (noroviruses and hepatitis E virus) were analysed for 1 year in inlet and outlet water to assess the removal efficiency of a lagooning system. We observed 2.58 (1.17-4.59) and 1.65 (0.15-3.14) log reductions in Escherichia coli (EC) and intestinal enterococci (IE), respectively, between the inlet and outlet samples. Genomic copies of the viruses were log reduced by 1.18 (0.24-2.93), 0.64 (0.12-1.97), 0.45 (0.04-2.54) and 0.72 (0.22-2.50) for human adenovirus (HAdV), JC polyomavirus (JCPyV) and human noroviruses (NoV GI and GII), respectively. No regrowth of opportunistic pathogens was observed within the system. FLA, detected in all samples, did not show a clear trend. The reduction of faecal pathogens was irregular with 6 out of 12 samples and 4 out of 12 samples exceeding the EC and IE values, specified in the Spanish legislation for reclaimed water (RD 1620/2007). This data evidences that there is a need for more studies to evaluate the removal mechanisms of lagooning systems in order to optimize pathogen reduction. Moreover, surveillance of water used to irrigate raw edible vegetables should be conducted to ensure the fulfilment of the microbial

  2. A microbiological survey of fresh meat in the supermarket trade. Part 2: beef retail cuts.

    Science.gov (United States)

    Nortjé, G L; Nel, L; Jordaan, E; Naudé, R T; Holzapfel, W H; Grimbeek, R J

    1989-01-01

    An assessment was made of the microbiological quality of the final product (different retail cuts), produced by two different retail supermarket groups (A & B). The influence of sanitary conditions on the microbiological quality of the final product was evaluated, and the possible influences on shelf life were determined. Supermarket group A (Sup group A) received carcasses with significantly lower surface counts of micro-organisms than supermarket group B (Sup group B), while the latter had a more efficient overall sanitation programme than supermarket group A. Five cuts were monitored for the presence of total aerobic counts, psychrotrophic counts, lactobacilli, Enterobacteriaceae and numbers of Pseudomonadaceae present. A shelf life study was also executed by repeating these enumerations on the same meat samples after refrigerated storage at 5°C for 2 and 4 days, respectively. It is generally accepted that a good refrigeration or chilling regime will preserve the inherent meat quality, but in this study it was found that neither served as a guarantee of quality. The more stringent hygiene at retail level of Sup group B yielded consistently lower mean counts of the different bacterial groups for all the meat cuts monitored and, thus meat with an extended shelf life. The total count (at 30°C) on meat cuts was the highest, followed by the psychrotrophs, the Pseudomonadaceae the Enterobacteriaeae and the lactobacilli. Minced meat generally had the highest mean aerobic total microbial counts. This count on minced meat might be a suitable indicator for monitoring the overall sanitary condition of a retail premises. The results re-emphasized the multi-factorial complexity of fresh meat quality and shelf life. The microbial quality of the raw material (carcasses), the maintenance of the cold chain, sanitary condition of premises, equipment and personnel surfaces and general management practices are factors that collectively determine the microbiological quality of the

  3. Uses of irradiation or sodium tripolyphosphate for shelf-life extension of quail carcasses

    International Nuclear Information System (INIS)

    this investigation aims to study the possibility of using gamma iraadiation or sodium tripolyphoshate (STPP) for increasing shelf-life of quail carcasses during cold storage. The quail carcasses were gamma irradiated at 4, 6 and 8 kGy or soaked in 3% STPP. The effect of these treatments on the organoleptic, microbiological aspects and chemical properties of samples under investigation were evaluated during cold storage (4± 1C degree). The results indicated that, the chemical compositions of samples did not alter by gamma irradiation or soaking in STPP. Futhermore, treatment had no deterious effects on the organoleptic properties of quail samples. Meanwhile, irradiation of samples at doses of 4, 6 and 8 kGy or soaking in STPP greatly reduced its microbial count and prolonged the shelf-life for 15,21,24 and 9 days at 4 ± 1 Cdegree in comparison with 6 days for control samples. Moreover, the acid value (A.V), peroxide value (P.V) and thiobarbituric acid (TBA) value were increased after irradiation, while total volatile basic nitrogen (TVBN) content had no real change by this treatment. On the other hand, soakking in STPP treatment decreased these values and gradual increases were observed during cold storage

  4. Learning about Foodborne Pathogens: Evaluation of Student Perceptions of Group Project Work in a Food Microbiology Course

    Science.gov (United States)

    Turner, Mark S.

    2009-01-01

    This study examined the experiences of students in an active learning group work exercise in an introductory food microbiology course involving the study of foodborne pathogens. Small groups were required to access, analyze, and present information regarding a single food poisoning bacterium. The presentations contained features and…

  5. Tenderness of Pork Muscles as Influenced by Chilling Rate and Altered Carcass Suspension

    DEFF Research Database (Denmark)

    Kirkegaard, Elin; Møller, Anders Juel

    1987-01-01

    Evaluation of tenderness improvements in porcine muscles (longissimus dorsi, semimembranosus, biceps femoris) in 72 carcasses by using combinations of three different chilling rates and two different suspennnsion methods....

  6. Microbiological Evaluation of Household Drinking Water Treatment in Rural China Shows Benefits of Electric Kettles: A Cross-Sectional Study.

    Directory of Open Access Journals (Sweden)

    Alasdair Cohen

    Full Text Available In rural China ~607 million people drink boiled water, yet little is known about prevailing household water treatment (HWT methods or their effectiveness. Boiling, the most common HWT method globally, is microbiologically effective, but household air pollution (HAP from burning solid fuels causes cardiovascular and respiratory disease, and black carbon emissions exacerbate climate change. Boiled water is also easily re-contaminated. Our study was designed to identify the HWT methods used in rural China and to evaluate their effectiveness.We used a geographically stratified cross-sectional design in rural Guangxi Province to collect survey data from 450 households in the summer of 2013. Household drinking water samples were collected and assayed for Thermotolerant Coliforms (TTC, and physicochemical analyses were conducted for village drinking water sources. In the winter of 2013-2104, we surveyed 120 additional households and used remote sensors to corroborate self-reported boiling data.Our HWT prevalence estimates were: 27.1% boiling with electric kettles, 20.3% boiling with pots, 34.4% purchasing bottled water, and 18.2% drinking untreated water (for these analyses we treated bottled water as a HWT method. Households using electric kettles had the lowest concentrations of TTC (73% lower than households drinking untreated water. Multilevel mixed-effects regression analyses showed that electric kettles were associated with the largest Log10TTC reduction (-0.60, p<0.001, followed by bottled water (-0.45, p<0.001 and pots (-0.44, p<0.01. Compared to households drinking untreated water, electric kettle users also had the lowest risk of having TTC detected in their drinking water (risk ratio, RR = 0.49, 0.34-0.70, p<0.001, followed by bottled water users (RR = 0.70, 0.53-0.93, p<0.05 and households boiling with pots (RR = 0.74, 0.54-1.02, p = 0.06.As far as we are aware, this is the first HWT-focused study in China, and the first to quantify the

  7. Evaluation of the use of radiation in microbiological control in tomato (Lycopersicon esculentum mill.) sold in the CEASA, Pernambuco

    Energy Technology Data Exchange (ETDEWEB)

    Vicalvi, Maria Claudia V.; Solidonio, Evelyne G.; Silva, Marcio de Albuquerque; Costa Junior, Carlos Eduardo de Oliveira; Alcantara, Moacir Paulo [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Pos-graduation program in Energetics and Nuclear Tecnologies; Miranda, Rita de Cassia M. de [Universidade Federal de Sergipe (UFS), Aracaju, SE (Brazil). Lab. of Chromatographic Analysis and Flavor; Sena, Kesia Xisto F.R. de [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. of Antibiotics; Colaco, Waldeciro, E-mail: wcolaco@ufpe.com.b [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. of Nuclear Energy

    2011-07-01

    Tomato is one of the most consumed fruits in the world and also one of the agricultural products with most losses due to its high perishability. Nevertheless, there are only a few studies on the post-harvest life of tomatoes and therefore are still many undefined parameters related to the treatment and shelf life of different cultivars of that fruit. Its contamination occurs from the pre-to post-harvest crop. The diseases caused by food borne pathogenic microorganisms are a serious public health problem. The bacteria of the Enterobacteriaceae are responsible for large outbreaks of gastroenteritis in humans that may occur due to bacteria per se or by toxins produced by them. Thus, the development of technologies that make the food safe for human consumption has been growing in recent years. Preservation methods employ physical or chemical processes. Among such methods, irradiation is a preventive treatment for food safety. Irradiation can reduce post-harvest losses by killing insect pests in fruits, grains, spices, or by reducing food spoilage organisms by inhibiting the sprouting of plants and delaying the ripening of fruits. The objective of this research was to evaluate the potential of using different doses of gamma radiation to treat tomatoes sold in CEASA-PE and to perform the overall count of microorganisms in control and irradiated samples. The study used two lots containing 60 tomatoes such that 15 of them were used as the control group and 45 were irradiated with one of 3 different doses (1.0 kGy, 1.5 kGy and 2.0 kGy). For microbiological analysis, the tomatoes were sliced, and weighed (after removal of peel) in order to obtain samples weighing 25g. Each sample was added to an Erlenmeyer containing sterile water by agitation for 15 minutes. Aliquots of the washings were sown for exhaustion in sheep blood agar. After growth, colony counting was performed. The results revealed contamination in all tomato groups analyzed with the control groups exhibiting

  8. Genetic parameters for EUROP carcass traits within different groups of cattle in Ireland.

    Science.gov (United States)

    Hickey, J M; Keane, M G; Kenny, D A; Cromie, A R; Veerkamp, R F

    2007-02-01

    The first objective of this study was to test the ability of systems of weighing and classifying bovine carcasses used in commercial abattoirs in Ireland to provide information that can be used for the purposes of genetic evaluation of carcass weight, carcass fatness class, and carcass conformation class. Secondly, the study aimed to test whether genetic and phenotypic variances differed by breed of sire. Variance components for carcass traits were estimated for crosses between dairy cows and 8 breeds of sire commonly found in the Irish cattle population. These 8 breeds were Aberdeen Angus, Belgian Blue, Charolais, Friesian, Hereford, Holstein, Limousin, and Simmental. A multivariate animal model was used to estimate genetic parameters within the Holstein sire breed group. Univariate analyses were used to estimate variance components for the remaining 7 sire breed groups. Multivariate sire models were used to formally test differences in genetic variances in sire breed groups. Field data on 64,443 animals, which were slaughtered in commercial abattoirs between the ages of 300 and 875 d, were analyzed in 8 analyses. Carcass fat class and carcass conformation class were measured using the European Union beef carcass classification system (EUROP) scale. For all 3 traits, the sire breed group with the greatest genetic variance had a value of more than 8 times the sire breed group with least genetic variance. Heritabilities ranged from zero to moderate for carcass fatness class (0.00 to 0.40), from low to moderate for carcass conformation class (0.04 to 0.36), and from low to high for carcass weight (0.06 to 0.65). Carcass weight was the most heritable (0.26) of the 3 traits. Carcass conformation class and carcass fatness class were equally heritable (0.17). Genetic and phenotypic correlations were all positive in the Holstein sire breed group. The genetic correlations varied from 0.11 for the relationship between carcass weight and carcass fatness class to 0.44 for the

  9. Effect of sun mushroom extract in pork sausage and evaluation of the oxidative and microbiological stability of the product

    Directory of Open Access Journals (Sweden)

    Flávia Santi Stefanello

    2015-02-01

    Full Text Available The prevention of lipid oxidation is one of the meat industry’s target and, consequently, the search for natural antioxidants has been increased in last years. Thus, the aim of this study was to determine the effect of hydroethanolic extract from sun mushroom (Agaricus blazei Murrill on the oxidative and microbiological stability of pork meat sausage during storage at 4ºC. The extract was added to sausages in 0 %, 0.5 %, 1.0 % and 2.0 % (v/w concentrations. There was done the proximate composition, pH, instrumental color (L*, a*, b*, C* e h*, TBARS (Thiobarbituric Acid Reactive Substances and microbiological analyzes. Data were subjected to analysis of variance, in randomized completely design. The results from proximate composition and microbiological analyzes at day 0 were according to the Brazilian legislation. At 1st day of storage, control treatment showed higher (p<0,05 a* value (red than the other treatments, while b* value (yellow has increased (p<0,05 during the storage period in all treatments. At 21st day of storage, TBARS values of sausages with 2.0 % extract addition was lower (0,705±0,01 mg MDA/kg sample (p<0,05 than the control (1,097±0,11mg MDA/kg sample. The extract has not shown effect on the microbiological stability of the sausages. Nevertheless, the hydroethanolic extract from sun mushroom was effective on the oxidative stability of pork meat sausage when added in a 2.0 % concentration, improving its shelf-life up to 21 days of storage at 4 °C, and it is possible the use as a natural antioxidant source.

  10. Safety Evaluation of Sous Vide-Processed Products with Respect to Nonproteolytic Clostridium botulinum by Use of Challenge Studies and Predictive Microbiological Models

    OpenAIRE

    Hyytiä-Trees, Eija; Skyttä, Eija; Mokkila, Mirja; Kinnunen, Arvo; Lindström, Miia; Lähteenmäki, Liisa; Ahvenainen, Raija; Korkeala, Hannu

    2000-01-01

    Sixteen different types of sous vide-processed products were evaluated for safety with respect to nonproteolytic group II Clostridium botulinum by using challenge tests with low (2.0-log-CFU/kg) and high (5.3-log-CFU/kg) inocula and two currently available predictive microbiological models, Food MicroModel (FMM) and Pathogen Modeling Program (PMP). After thermal processing, the products were stored at 4 and 8°C and examined for the presence of botulinal spores and neurotoxin on the sell-by da...

  11. Use of real-time ultrasound to evaluate live animal carcass traits in young performance-tested beef bulls.

    Science.gov (United States)

    Bergen, R D; McKinnon, J J; Christensen, D A; Kohle, N; Belanger, A

    1997-09-01

    Young bulls were ultrasonically scanned to 1) study breed differences for 12th rib fat depth (USFAT) and longissimus muscle area (USREA), 2) evaluate the nature and predictability of USFAT and USREA development, and 3) determine the effect of age (YFAT and YREA, respectively) or weight (WREA) adjustments on USFAT and USREA. Angus (AA), Charolais (CH), Hereford (HH), Shorthorn (SS), and Simmental (SM) bulls (n = 886) were studied in 2 yr at two stations. Breed differences were observed for end of test USFAT (AA = SS > HH > CH = SM, P SS = AA > HH, P .05), but USREA development was linear (P < .05). Prediction equations were characterized by low r2 and high residual standard deviation (RSD) values, although those for d-84 USREA had r2 values from .63 to .70 and RSD values from 4.09 to 5.80 cm2. High associations were obtained between end of test USFAT with YFAT (r = .83 to .91, P < .05) and USREA with YREA (r = .74 to .84, P < .05) but not for end of test USREA with WREA (r = .31 to .56, P < .05). These results indicate that end of test ultrasound measurements may be a useful addition to performance testing programs. PMID:9303445

  12. Effect of exposure to stress conditions on propidium monoazide (PMA)-qPCR based Campylobacter enumeration in broiler carcass rinses.

    Science.gov (United States)

    Duarte, A; Botteldoorn, N; Coucke, W; Denayer, S; Dierick, K; Uyttendaele, M

    2015-06-01

    Campylobacter quantification by qPCR is unable to distinguish viable vs. dead cells in contrast to the culture-based ISO 10272-2 reference method. Propidium monoazide (PMA) has been used to overcome this disadvantage. A Campylobacter PMA-qPCR enumeration method was evaluated for its consistency and compared to the culture-based enumeration for both artificially and natural contaminated broiler carcass rinses. The PMA effect was further evaluated on stressed cells. Five conditions, commonly encountered during the slaughter process and storage (acid, heat, cold, oxidation and freezing), were inflicted to the broiler carcass rinses artificially contaminated with Campylobacter jejuni or Campylobacter coli. A better correlation between the reference method and the qPCR enumeration was obtained when PMA was used. The two cultured-based methods used showed a significant CFU reduction for heat, cold and acid stresses although the PMA-qPCR enumeration showed that viable bacteria were underestimated. Freezing showed the highest reduction effect, while the reduction extend was also overestimated by the microbiological enumeration procedure. Exposure to a mild oxidative stress was the only stress condition applied at temperatures permitting adaptation of Campylobacter and did not lead to either reduction in CFU nor in the PMA-qPCR signal.

  13. Crude glycerin in diets for wethers in feedlot: intake, digestibility, performance, carcass and meat traits

    Directory of Open Access Journals (Sweden)

    Mauriceia Costa Carvalho Barros

    2015-02-01

    Full Text Available The aim was to evaluate the effects of crude glycerin levels (CG (0, 2.65, 5.33, 8.06 and 10.84% in dry matter basis on intake, digestibility, performance, carcass morphometric measurements and meat quality of sheep finished in feedlot. Twenty five crossbred Dorper x St. Ines wethers, with 24 ± 2.0 kg average weight were housed in individual pens. The experimental design was completely randomized. Levels of CG in gross diet caused a decreasing linear effect (P 0.05 levels of glycerin on animal performance. For morphometric measurements of carcass and meat characteristics, no significant difference (P>0.05 levels of glycerin for hot carcass weight, chilled carcass weight, hot carcass yield, Chilled carcass yields, loss cooling, state of greasing, carcass length, leg length, depth of leg, chest depth, loin eye area, texture, marbling and color. However, the influence was observed (P<0.05 of increasing levels of crude glycerin on carcass conformation and carcass fat thickness. The use of crude glycerin reduced nutrient intake of the animals, however, did not influence the performance and carcass characteristics and is indicated its use in the diet of sheep

  14. Evaluation of different drinking water sources in Sokoto North-West Nigeria on performance, carcass traits and haematology of broiler chickens

    OpenAIRE

    Ibitoye, E. B.; Y. U. Dabai; L. Mudi

    2013-01-01

    Aim: The study examined the effect of different drinking water sources on performance, carcass characteristicsand haematology of broiler chickens.Materials and Methods: 63 unsexed day-old broiler chicks were randomly grouped into three treatment groups(Treatments 1, 2 and 3), each consisting of three replicates of seven chicks each. Treatments 1, 2 and 3 were givenwater from various sources: pipe borne water; borehole water and well water, respectively from day-old to sevenweeks of age. The w...

  15. Growth performance, carcass and non-carcass characteristics of Mubende and Mubende×Boer crossbred goats under different feeding regimes

    DEFF Research Database (Denmark)

    Asizua, D.; Mpairwe, D.; Kabi, F.;

    2014-01-01

    evaluated the effects of supplementing grazing Mubende goats and their crossbreds (Mubende×Boer) with concentrates on growth, carcass and non-carcass characteristics. A 2×3 factorial treatment arrangement was used to randomly allocate 96 pure Mubende and Mubende×Boer castrates (mean±SE; 31.3±2.2 kg initial...

  16. Tenderness of Pork Muscles as Influenced by Chilling Rate and Altered Carcass Suspension

    DEFF Research Database (Denmark)

    Kirkegaard, Elin; Møller, Anders Juel

    1987-01-01

    Evaluation of tenderness improvements in porcine muscles (longissimus dorsi, semimembranosus, biceps femoris) in 72 carcasses by using combinations of three different chilling rates and two different suspennnsion methods.......Evaluation of tenderness improvements in porcine muscles (longissimus dorsi, semimembranosus, biceps femoris) in 72 carcasses by using combinations of three different chilling rates and two different suspennnsion methods....

  17. Avaliação de carcaças de suínos da raça Large White utilizando medidas convencionais Evaluation of Large White pig carcass using conventional measures

    Directory of Open Access Journals (Sweden)

    Rilke Tadeu Fonseca de Freitas

    2004-12-01

    swine, data of lean meat yield (MY, fat yield (FY, dressing percentage (DP, carcass length for the Brazilian Method of Carcass Classification (CLBM, carcass length for the American Method of Carcass Classification (CLAM, average backfat thickness (ABT, backfat thickness 4 cm from the dorsal mid-line (P1, backfat thickness 6.5 cm from the dorsal mid-line (P2, backfat thickness 8 cm from the dorsal mid-line (P3, loin eye area (LEA, lean:fat ratio (LFR and ham yield (HY were used, obtained in 664 Large White swine. Initially, in order to make clear the importance and possible relations structures among variables, the cluster analysis was used. Afterwards, the multiple regression linear model was used, using the SAS package. Firstly, it was estimated the partial correlation (Type II of each trait to evaluate the importance of the quality variables of the carcass. Secondly, a procedure of adjusting equation selection named Backward was used. It was verified that, except for DP, all the measures of carcass classification evaluated presented correlation with MY and FY. The measures ABT, P1, P2, P3 and LFT were inversely correlated with MY and directly correlated with FY. The opposite was observed for the measures CLBM, CLAM, LEA and HY. ABT, AOL, RCG and RP are just enough to evaluate the quality of the carcass in terms of lean meat yield, and, in terms of FY, besides these measured previous it's necessary the evaluation of P1. The measures of loin eye area and average backfat thickness, as proposed by MBCC, shown as being what best evaluates the amount of meat and fat in the carcass.

  18. Evaluation of the HACCP System in a University Canteen: Microbiological Monitoring and Internal Auditing as Verification Tools

    Directory of Open Access Journals (Sweden)

    Stefano Tavoletti

    2013-04-01

    Full Text Available Food safety is essential in mass catering. In Europe, Regulation (EC No. 852/2004 requires food business operators to put in place, implement and maintain permanent procedures based on Hazard Analysis and Critical Control Point (HACCP principles. Each HACCP plan is specifically implemented for the processing plant and processing methods and requires a systematic collection of data on the incidence, elimination, prevention, and reduction of risks. In this five-year-study, the effectiveness of the HACCP plan of a University canteen was verified through periodic internal auditing and microbiological monitoring of meals, small equipment, cooking tools, working surfaces, as well as hands and white coats of the canteen staff. The data obtained revealed no safety risks for the consumers, since Escherichia coli, Salmonella spp. and Listeria monocytogenes were never detected; however, a quite discontinuous microbiological quality of meals was revealed. The fluctuations in the microbial loads of mesophilic aerobes, coliforms, Staphylococcus aureus, Bacillus cereus, and sulphite-reducing clostridia were mainly ascribed to inadequate handling or processing procedures, thus suggesting the need for an enhancement of staff training activities and for a reorganization of tasks. Due to the wide variety of the fields covered by internal auditing, the full conformance to all the requirements was never achieved, though high scores, determined by assigning one point to each answer which matched with the requirements, were achieved in all the years.

  19. Evaluation of the HACCP system in a university canteen: microbiological monitoring and internal auditing as verification tools.

    Science.gov (United States)

    Osimani, Andrea; Aquilanti, Lucia; Tavoletti, Stefano; Clementi, Francesca

    2013-04-01

    Food safety is essential in mass catering. In Europe, Regulation (EC) No. 852/2004 requires food business operators to put in place, implement and maintain permanent procedures based on Hazard Analysis and Critical Control Point (HACCP) principles. Each HACCP plan is specifically implemented for the processing plant and processing methods and requires a systematic collection of data on the incidence, elimination, prevention, and reduction of risks. In this five-year-study, the effectiveness of the HACCP plan of a University canteen was verified through periodic internal auditing and microbiological monitoring of meals, small equipment, cooking tools, working surfaces, as well as hands and white coats of the canteen staff. The data obtained revealed no safety risks for the consumers, since Escherichia coli, Salmonella spp. and Listeria monocytogenes were never detected; however, a quite discontinuous microbiological quality of meals was revealed. The fluctuations in the microbial loads of mesophilic aerobes, coliforms, Staphylococcus aureus, Bacillus cereus, and sulphite-reducing clostridia were mainly ascribed to inadequate handling or processing procedures, thus suggesting the need for an enhancement of staff training activities and for a reorganization of tasks. Due to the wide variety of the fields covered by internal auditing, the full conformance to all the requirements was never achieved, though high scores, determined by assigning one point to each answer which matched with the requirements, were achieved in all the years.

  20. Evaluation of the HACCP system in a university canteen: microbiological monitoring and internal auditing as verification tools.

    Science.gov (United States)

    Osimani, Andrea; Aquilanti, Lucia; Tavoletti, Stefano; Clementi, Francesca

    2013-04-01

    Food safety is essential in mass catering. In Europe, Regulation (EC) No. 852/2004 requires food business operators to put in place, implement and maintain permanent procedures based on Hazard Analysis and Critical Control Point (HACCP) principles. Each HACCP plan is specifically implemented for the processing plant and processing methods and requires a systematic collection of data on the incidence, elimination, prevention, and reduction of risks. In this five-year-study, the effectiveness of the HACCP plan of a University canteen was verified through periodic internal auditing and microbiological monitoring of meals, small equipment, cooking tools, working surfaces, as well as hands and white coats of the canteen staff. The data obtained revealed no safety risks for the consumers, since Escherichia coli, Salmonella spp. and Listeria monocytogenes were never detected; however, a quite discontinuous microbiological quality of meals was revealed. The fluctuations in the microbial loads of mesophilic aerobes, coliforms, Staphylococcus aureus, Bacillus cereus, and sulphite-reducing clostridia were mainly ascribed to inadequate handling or processing procedures, thus suggesting the need for an enhancement of staff training activities and for a reorganization of tasks. Due to the wide variety of the fields covered by internal auditing, the full conformance to all the requirements was never achieved, though high scores, determined by assigning one point to each answer which matched with the requirements, were achieved in all the years. PMID:23594937

  1. Evaluation of routinely reported surgical site infections against microbiological culture results: a tool to identify patient groups where diagnosis and treatment may be improved

    Directory of Open Access Journals (Sweden)

    Kievit Job

    2009-11-01

    Full Text Available Abstract Background Surgeons may improve their decision making by assessing the extent to which their initial clinical diagnosis of a surgical site infection (SSI was supported by culture results. Aim of the present study was to evaluate routinely reported SSI by surgeons against microbiological culture results, to identify patient groups with lower agreement where decision making may be improved. Methods 701 admissions with SSI were reported by surgeons in a university medical centre in the period 1997-2005, which were retrospectively checked for microbiological culture results. Reporting a SSI was conditional on treatment being given (e.g. antibiotics and was classified by severity. To identify specific patient groups, patients were classified according to the surgery group of the first operation during admission (e.g. trauma. Results Of all reported SSI, 523 (74.6% had a positive culture result, 102 (14.6% a negative culture result and 76 (10.8% were classified as unknown culture result (due to no culture taken. Given a known culture result, reported SSI with positive culture results less often concerned trauma patients (16% versus 26%, X2 = 4.99 p = 0.03 and less severe SSI (49% versus 85%, X2 = 10.11 p Conclusion Routine reporting of SSI was mostly supported by culture results. However, this support was less often found in trauma patients and less severe SSI, thereby giving surgeons feedback that diagnosis and treatment may be improved in these cases.

  2. Physical and Microbiological Evaluation of Food Formulations From Malted and Fermented Maize (Zea mays L. Fortified with Defatted Sesame (Sesamun indicum L. Flour

    Directory of Open Access Journals (Sweden)

    D.I. Gernah

    2012-06-01

    Full Text Available The effects of malting and fermentation on some physical properties and microbiological composition and safety of maize/sesame food formulations were investigated. Malted and fermented maize flours were blended with defatted sesame flour, by material balancing, to give four food formulations (Unmalted maize + defatted sesame (UMS, malted maize + defatted sesame (MMS, un-malted fermented maize + defatted sesame (UFMS and malted fermented maize + defatted sesame (MFMS, which all contained 16 g protein and 9 g fat/100 g food. Viscosity, functional properties and microbiological composition of flours and gruels from the food formulations were evaluated using standard methods of analysis. Malting and fermentation significantly (p<0.05 decreased gruel viscosities from 318.00 to 210.70 cP, packed bulk density from 1.08 to 0.97 g/mL and swelling index from 4.43 to 2.93 mL/g; while increasing water absorption capacity from 3.30 to 4.07 g/g and reconstitution index from 5.02 to 6.13 mL/g in UMS and MFMS samples respectively. Total viable counts, yeasts and moulds all increased significantly (p<0.05 with malting and fermentation. Enterobacteriaceae and Staphylococcus species were predominant in the non-fermented products, while yeast dominated in the fermented products, whose gruels also inhibited growth of Staphylococcus in challenge tests.

  3. Effects of Duroc Breeding Lines on Carcass Composition and Meat Quality

    OpenAIRE

    Choi, Jung-Seok; Jin, Sang-Keun; Choi, Yang-Il; Lee, Jae-Joon

    2015-01-01

    This study was performed to investigate the carcass composition and pork quality of Duroc breeding lines in Korea. A total of 200 Duroc pigs were used, and those were originated from four different great-grandparent (GGP) breeding stock farms (L1: N farm, L2: W farm, L3: S farm, L4: R farm). The carcasses of pigs from these farms were collected, and meat quality traits were evaluated. L1 and L2 had smaller carcass weights and thin backfat, whereas L3 and L4 had heavy carcass weights and thick...

  4. Genetic parameters for carcass weight, conformation and fat in five beef cattle breeds.

    Science.gov (United States)

    Kause, A; Mikkola, L; Strandén, I; Sirkko, K

    2015-01-01

    Profitability of beef production can be increased by genetically improving carcass traits. To construct breeding value evaluations for carcass traits, breed-specific genetic parameters were estimated for carcass weight, carcass conformation and carcass fat in five beef cattle breeds in Finland (Hereford, Aberdeen Angus, Simmental, Charolais and Limousin). Conformation and fat were visually scored using the EUROP carcass classification. Each breed was separately analyzed using a multitrait animal model. A total of 6879-19 539 animals per breed had phenotypes. For the five breeds, heritabilities were moderate for carcass weight (h 2=0.39 to 0.48, s.e.=0.02 to 0.04) and slightly lower for conformation (h 2=0.30 to 0.44, s.e.=0.02 to 0.04) and carcass fat (h 2=0.29 to 0.44, s.e.=0.02 to 0.04). The genetic correlation between carcass weight and conformation was favorable in all breeds (r G=0.37 to 0.53, s.e.=0.04 to 0.05), heavy carcasses being genetically more conformed. The phenotypic correlation between carcass weight and carcass fat was moderately positive in all breeds (r P=0.21 to 0.32), implying that increasing carcass weight was related to increasing fat levels. The respective genetic correlation was the strongest in Hereford (r G=0.28, s.e.=0.05) and Angus (r G=0.15, s.e.=0.05), the two small body-sized British breeds with the lowest conformation and the highest fat level. The correlation was weaker in the other breeds (r G=0.08 to 0.14). For Hereford, Angus and Simmental, more conformed carcasses were phenotypically fatter (r P=0.11 to 0.15), but the respective genetic correlations were close to zero (r G=-0.05 to 0.04). In contrast, in the two large body-sized and muscular French breeds, the genetic correlation between conformation and fat was negative and the phenotypic correlation was close to zero or negative (Charolais: r G=-0.18, s.e.=0.06, r P=0.02; Limousin: r G=-0.56, s.e.=0.04, r P=-0.13). The results indicate genetic variation for the genetic

  5. Evaluation of carcass traits, non-carcass components and 12th rib analysis of hair sheep supplemented with phosphorus Avaliação das características de carcaça, constituintes corporais e da 12ª costela de ovinos deslanados suplementados com fósforo

    Directory of Open Access Journals (Sweden)

    Helder Louvandini

    2006-04-01

    Full Text Available The aim of this study was to evaluate the performance of sheep supplemented with phosphorus (P using carcass traits and analysis of 12th rib. Twenty male lambs weighing 13.88 ± 2.51 kg and grazing Andropogon gayanus pasture were divided in two treatments of 10 animals each. In group P, animals were supplemented with 3 g phosphorus/animal/day, while in group NP no P supplementation was given. After 82 days the animals were slaughtered and carcass measurements were taken. The 12th rib was also removed for analysis. No significant differences in body weight, carcass yield, or commercial cut weights were found between both treatments, except for liver and kidneys that were heavier in the supplemented group (p=0.0954. The 12th rib analysis showed that the group P had higher rib, bone, and muscle weights when compared to animals not supplemented with P. Other measurements such as carcass fatness and rib eye area were not significantly affected by treatments as well as contents of crude protein, ether extract, ash and P. It was shown that P supplementation improved performance in young sheep, including bone and muscle tissue and liver and kidney weights.Este trabalho foi realizado com a finalidade de avaliar o desempenho de ovinos suplementados com fósforo (P, utilizando-se as análises das características de carcaça e da 12ª costela. Foram utilizados 20 ovinos com 13,88 ± 2,51 kg de PV, mantidos em pastagem de Andropogon gayanus, distribuídos em dois tratamentos com dez animais: um com suplementação de 3g/fósforo/animal/dia e o outro sem suplementação. Após 82 dias, os animais foram abatidos, tomando-se as medidas da carcaça e da 12ª costela, para posterior análise. Não foram encontradas diferenças significativas entre os valores de peso vivo, rendimento de carcaça, cobertura de gordura e cortes comerciais entre os dois tratamentos, exceto os pesos do fígado e dos rins, que foram superiores (p=0,0954 no grupo suplementado. Na an

  6. The Evaluation and Improvement of Microbial Analysis Methods and Techniques in the Johnson Space Center Microbiology Laboratory

    Science.gov (United States)

    St. Pierre, Ashley L.; Smith, Melanie J.; Dunbar, Brandon J.; Ott, C. Mark

    2016-01-01

    Microbial contamination is a risk to crew health both on the ground and on the International Space Station. The methods and techniques of microbial analysis for surfaces and items on board a spacecraft are an important aspect of ensuring that crew health is not at unnecessary risk. This series of projects seeks to improve and optimize microbial analysis methods, such as the recovery of bacteria from difficult surfaces, and the analysis of long-term bacterial isolate storage techniques. These projects will help the Johnson Space Center Microbiology laboratory be more efficient at identifying and addressing microbial concerns related to spaceflight and on the ground. Fabrics are substantially more problematic for microbial monitoring than steel or glass surfaces, as bacteria tend to get trapped in the fibers. To better understand which microbial sampling method is most efficient, various methods of recovery were attempted, and the processes were revised based on the results. The final phase of testing applied the knowledge gained from the resulting methods to an acoustic blanket that is currently being used on the International Space Station, with future use slated for the Orion spacecraft. In this phase, the sample materials were inoculated with bacterial suspensions, vortexed with phosphate-saline buffer, and the liquid was plated on tryptic soy agar. The results from the test of the acoustic blanket indicate that not only is microbial growth possible on fabric that is treated for moisture-resistance, but also that contamination can occur if aseptic handling procedures are not followed. Interestingly, out of three hundred and ninety (390) plates, only seventeen (17) showed growth (4.36%), and recovery of the Staphylococcus epidermidis used to inoculate the samples only occurred on four (4) of the plates. The growth on the remaining plates was contamination by various microbes, including those commonly found on the skin and in soil. This shows that 2 while bacterial

  7. The use of seemingly unrelated regression (SUR) to predict the carcass composition of lambs

    DEFF Research Database (Denmark)

    Cadavez, Vasco A. P.; Henningsen, Arne

    fat, and bone plus remainder were otained. Our models for carcass composition were fitted using the SUR estimator which is novel in this area. The results were compared to OLS estimates and evaluated by several statistical measures. As the models are intended to predict carcass composition, we...

  8. Ultrasound use for body composition and carcass quality assessment in cattle and lambs

    Science.gov (United States)

    Genetic evaluation for carcass quality traits has evolved over time, in large part due to introduction of new technology such as ultrasound measures of body composition. Ultrasound measured body composition traits emulate important carcass traits, are very informative for selection purposes, are ac...

  9. Performance and carcass yield of crossbred dairy steers fed diets with different levels of concentrate.

    Science.gov (United States)

    da Silva, Gabriel Santana; Chaves Véras, Antônia Sherlanea; de Andrade Ferreira, Marcelo; Moreira Dutra, Wilson; Menezes Wanderley Neves, Maria Luciana; Oliveira Souza, Evaristo Jorge; Ramos de Carvalho, Francisco Fernando; de Lima, Dorgival Morais

    2015-10-01

    The objective of this study was to evaluate the influence of diets with increasing concentrate levels (170, 340, 510 and 680 g/kg of total dry matter) on dry matter intake, digestibility, performance and carcass characteristics of 25 Holstein-Zebu crossbred dairy steers in a feedlot. A completely randomized design was used, and data were submitted to analysis of variance and regression. The dry matter intake and digestibility coefficients of all nutrients increased linearly. The total weight gain and average daily gain added 1.16 kg and 9.90 g, respectively, for each 10 g/kg increase in concentrate. The empty body weight, hot carcass weight and cold carcass weight responded linearly to increasing concentrate. The hot carcass yield and cold carcass yield, gains in empty body weight and carcass gain were also influenced, as were the efficiencies of carcass deposition and carcass deposition rate. It is concluded that increasing concentrate levels in feedlot diets increase the intake and digestibility of dry matter and other nutrients, improving the feed efficiency, performance and physical characteristics of the carcass. Furthermore and of importance concerning the climate change debate, evidence from the literature indicates that enteric methane production would be reduced with increasing concentrate levels such as those used. PMID:26065699

  10. Virtual dissection of pig carcasses

    DEFF Research Database (Denmark)

    Vester-Christensen, Martin; Erbou, Søren Gylling Hemmingsen; Hansen, Mads Fogtmann;

    2009-01-01

    This paper proposes the use of computed tomography (CT) as a reference method for estimating the lean meat percentage (LMP) of pig carcasses. The current reference is manual dissection which has a limited accuracy due to variability between butchers. A contextual Bayesian classification scheme...... is applied to classify volume elements of full body CT-scans of pig carcasses into three tissue types. A linear model describes the relation between voxels and the full weight of the half carcass, which can be determined more accurately than that of the lean meat content. Two hundred and ninety-nine half pig...... carcasses were weighed and CT-scanned. The explained variance of the model was R-2 = 0.9994 with a root-mean-squared error of prediction of 83.6 g. Applying this method as a reference will ensure a more robust calibration of sensors for measuring the LMP, which is less prone to variation induced by manual...

  11. Microbiological and chemical evaluation of Helix spp. snails from local and non-EU markets, utilised as food in Sardinia

    Directory of Open Access Journals (Sweden)

    Arianna Corda

    2014-04-01

    Full Text Available With this study, 28 pools of snails of the genus Helix, respectively Helix aspersa (n=24 and Helix vermiculata (n=4 were analysed. They were taken from snail farming and stores. The snails were from Sardinia, other regions of Italy, and from abroad. All the samples were examined as pool looking for these microbiological target: Salmonella spp., Listeria monocytogenes, Escherichia coli O157, Clostridium perfringens, Norovirus and Hepatitis A Virus (HAV. In the same pools, the concentration of cadmium and lead by inductively coupled plasma mass spectrometry was also determined. The levels of these heavy metals were quite high, especially for cadmium. Two samples were positive for Salmonella spp., while no sample was positive for Escherichia coli O157, HAV and Norovirus. Two samples were positive for Clostridium perfringens and 8 for Listeria monocytogenes. The microrganisms related to Listeria monocytogenes were identified using biochemical techniques, then serotyped and gene sequenced by multiple loci sequence typing technique. Furthermore, antimicrobial restistence was tested on the same samples.

  12. Microbiological evaluation of the efficacy of soapy water to clean hands: a randomized, non-inferiority field trial.

    Science.gov (United States)

    Amin, Nuhu; Pickering, Amy J; Ram, Pavani K; Unicomb, Leanne; Najnin, Nusrat; Homaira, Nusrat; Ashraf, Sania; Abedin, Jaynal; Islam, M Sirajul; Luby, Stephen P

    2014-08-01

    We conducted a randomized, non-inferiority field trial in urban Dhaka, Bangladesh among mothers to compare microbial efficacy of soapy water (30 g powdered detergent in 1.5 L water) with bar soap and water alone. Fieldworkers collected hand rinse samples before and after the following washing regimens: scrubbing with soapy water for 15 and 30 seconds; scrubbing with bar soap for 15 and 30 seconds; and scrubbing with water alone for 15 seconds. Soapy water and bar soap removed thermotolerant coliforms similarly after washing for 15 seconds (mean log10 reduction = 0.7 colony-forming units [CFU], P time to 30 seconds did not improve removal (P > 0.05). Scrubbing hands with water alone also reduced thermotolerant coliforms (mean log10 reduction = 0.3 CFU, P = 0.046) but was less efficacious than scrubbing hands with soapy water. Soapy water is an inexpensive and microbiologically effective cleansing agent to improve handwashing among households with vulnerable children. PMID:24914003

  13. Environmental Microbiology Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Environmental Microbiology Laboratory, located in Bldg. 644 provides a dual-gas respirometer for measurement of oxygen consumption and carbon dioxide evolution...

  14. ANALYTICAL MICROBIOLOGY LABORATORY

    Data.gov (United States)

    Federal Laboratory Consortium — This laboratory contains equipment that performs a broad array of microbiological analyses for pathogenic and spoilage microorganisms. It performs challenge studies...

  15. Genetic parameters for carcass traits and their live animal indicators in Simmental cattle.

    Science.gov (United States)

    Crews, D H; Pollak, E J; Weaber, R L; Quaas, R L; Lipsey, R J

    2003-06-01

    The objective of this study was to estimate parameters required for genetic evaluation of Simmental carcass merit using carcass and live animal data. Carcass weight, fat thickness, longissimus muscle area, and marbling score were available from 5,750 steers and 1,504 heifers sired by Simmental bulls. Additionally, yearling ultrasound measurements of fat thickness, longissimus muscle area, and estimated percentage of intramuscular fat were available on Simmental bulls (n = 3,409) and heifers (n = 1,503). An extended pedigree was used to construct the relationship matrix (n = 23,968) linking bulls and heifers with ultrasound data to steers and heifers with carcass data. All data were obtained from the American Simmental Association. No animal had both ultrasound and carcass data. Using an animal model and treating corresponding ultrasound and carcass traits separately, genetic parameters were estimated using restricted maximum likelihood. Heritability estimates for carcass traits were 0.48 +/- 0.06, 0.35 +/- 0.05, 0.46 +/- 0.05, and 0.54 +/- 0.05 for carcass weight, fat thickness, longissimus muscle area, and marbling score, respectively. Heritability estimates for bull (heifer) ultrasound traits were 0.53 +/- 0.07 (0.69 +/- 0.09), 0.37 +/- 0.06 (0.51 +/- 0.09), and 0.47 +/- 0.06 (0.52 +/- 0.09) for fat thickness, longissimus muscle area, and intramuscular fat percentage, respectively. Heritability of weight at scan was 0.47 +/- 0.05. Using a bivariate weight model including scan weight of bulls and heifers with carcass weight of slaughter animals, a genetic correlation of 0.77 +/- 0.10 was obtained. Models for fat thickness, longissimus muscle area, and marbling score were each trivariate, including ultrasound measurements on yearling bulls and heifers, and corresponding carcass traits of slaughter animals. Genetic correlations of carcass fat thickness with bull and heifer ultrasound fat were 0.79 +/- 0.13 and 0.83 +/- 0.12, respectively. Genetic correlations of

  16. Microbiological and histopathological aspects of canine pyometra

    OpenAIRE

    Coggan, Jennifer Anne; Melville, Priscilla Anne; de Oliveira, Clair Motos; Faustino, Marcelo; Moreno, Andréa Micke; Benites, Nilson Roberti

    2008-01-01

    As pyometra is recognized as one of the main causes of disease and death in the bitch the purposes of this study were to evaluate microbiological and histopathological aspects of canine pyometra and to research the virulence factors of the E. coli isolates identifying possible risks to human health. The microbiological isolation from the intrauterine contents of 100 dogs with pyometra was carried out and the virulence factors in the E. coli strains were identified using PCR method. This study...

  17. Microbiology, philosophy and education.

    Science.gov (United States)

    O'Malley, Maureen A

    2016-09-01

    There are not only many links between microbiological and philosophical topics, but good educational reasons for microbiologists to explore the philosophical issues in their fields. I examine three broad issues of classification, causality and model systems, showing how these philosophical dimensions have practical implications. I conclude with a discussion of the educational benefits for recognising the philosophy in microbiology.

  18. Oral Microbiology and Immunology

    DEFF Research Database (Denmark)

    Dahlén, Gunnar; Fiehn, Nils-Erik; Olsen, Ingar

    , dental assistants and trainees may find it a useful source of reference. The contents are based on general microbiology and immunology. Oral microbiology is given particular attention, with examples relevant to oral infectious diseases. Each chapter opens with a relatively short pre-reading section...

  19. Microbiology, philosophy and education.

    Science.gov (United States)

    O'Malley, Maureen A

    2016-09-01

    There are not only many links between microbiological and philosophical topics, but good educational reasons for microbiologists to explore the philosophical issues in their fields. I examine three broad issues of classification, causality and model systems, showing how these philosophical dimensions have practical implications. I conclude with a discussion of the educational benefits for recognising the philosophy in microbiology. PMID:27465488

  20. Egg Microbiology Basics

    Science.gov (United States)

    Microbiology is the study of living microorganisms. This includes any single living animal not visible to the naked eye most of which are less than 0.1 mm in diameter. Bacteria, yeasts, molds, viruses, some algae and protozoans are considered microorganisms. Microbiology is a diverse field and fo...

  1. A microbiological evaluation of the use of denture cleansers in combination with an oral rinse in complete denture patients

    Directory of Open Access Journals (Sweden)

    Srinivasan Murali

    2010-01-01

    Full Text Available Background and Aim : Patients with complete dentures more occasionally neglect their denture and oral health by improper maintenance of dentures, resulting in bad oral and denture hygiene. This may lead to a host of multiple local and systemic problems such as stomatitis, bacterial and fungal infections, reservoirs for distant infections, denture stains and bad breath. Most denture wearers maintain their dentures with simple manual cleansing methods, which are not adequate to effectively sanitize the reservoir of microorganisms inhabiting the denture surfaces. The aim of this study was to compare the efficacy of the use of a denture cleanser and compare it with an adjunct use of chlorhexidine mouth rinse along with the denture cleanser and possibly suggest it as a suitable protocol. Materials and Methods : Two groups of complete denture patients were selected for the study. The total number of patients in each group was 12 and the total period of the study was 21 days. Three sets of microbiological samples were collected from each patient: before the start of the study, on day 8 and on day 21 of the study. The first sample was collected after an initial washover period (7 days with plain water before the start of the study and the second set after 1 week of study initiation, where group-1 followed protocol-I (denture cleanser only and group-2 followed protocol-II (denture cleanser and mouth rinse. A second washout period of 7 days followed and a crossover of the protocol was performed for the groups and followed for 1 week. Then, a third set of samples were collected. The colony-forming units were calculated for each patient for each sample and statistically analyzed. Friedman test for non-parametric analysis was employed for the comparison within the groups and a Mann-Whitney test was used for the statistical comparison between the groups. Results : There was a definite reduction in the bacterial numbers and a significant statistical difference

  2. Carcass or Tissue Packaging and Shipping

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — SOP on proper shipping of wildlife tissues to labs. Provides stepwise instructions and guidance on how to collect and ship wildlife carcasses, carcass parts, or...

  3. Assessing Body Condition from Fat on Carcass

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — SOP guiding assessment of body condition based on carcass fat. Provides stepwise instructions and photos on how to assess body condition from carcass fat

  4. Evaluation of biogenic amines levels, and biochemical and microbiological characterization of Italian-type salami sold in Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    Luiz Felipe Lopes dos Santos

    2015-08-01

    Full Text Available The objective of the present study was to evaluate the levels of biogenic amines (cadaverine, putrescine, tyramine, histamine, spermidine and spermine by high performance liquid chromatography (HPLC and the physicochemical (moisture, lipids, proteins, pH, water activity and fixed mineral residue and microbiological (lactic acid bacteria and aerobic heterotrophic mesophilic bacteria count characteristics of six Italian-type salami brands sold in the city of Niteroi (Rio de Janeiro, Brazil. The salami showed lactic acid bacteria count from 5.7 to 8.6 CFU•mL-1, and heterotrophic mesophilic bacteria count from 5.8 to 8.7 CFU•mL-1. Three brands showed moisture contents above 35% and one brand had protein content below 25%. The mean values obtained for the amines were: 197.43, 143.29, 73.02, 4.52, 90.66 and 36.17 mg•kg-1 for tyramine, putrescine, cadaverine, spermidine, histamine, and spermine respectively. Two brands presented histamine contents above the legal limit established in 100 mg•kg-1. We concluded that the evaluated salami presented a wide variation in the count of the bacterial groups with a predominance of lactic acid bacteria. The moisture contents indicate insufficient drying before commercialization and protein content had values below the minimum limit determined by the Brazilian legislation. Finally, the levels of biogenic amines found could cause adverse reactions in susceptible consumers, depending of the amount and frequency of intake of these products.

  5. Effect of Calf Health on Feedlot Performance and Carcass Value

    OpenAIRE

    Robson, Shana

    2007-01-01

    This paper addresses if calf health does affect feedlot performance and carcass value. The physiological aspects of this question as well as a regression analysis to further evaluate this problem will be addressed in this paper. A large set of data that can help to explain this health and value question has been made available for this study. These data were collected from the years 1990 - 2005 from a cattle feeding and carcass program in Coyle, Oklahoma as part of an Oklahoma State Universit...

  6. Carcass and Non-carcass Components of Priangan and Javanese Fat-tailed Rams Slaughtered at Mature Live Weight

    Directory of Open Access Journals (Sweden)

    M. Baihaqi

    2012-12-01

    Full Text Available Twenty-three of indigenous Priangan and Javanese Fat-tailed (JFT ram breeds were used to evaluate its carcass and non carcass components slaughtered at mature live weight. Five Priangan rams and six JFT rams were slaughtered at 32.5 kg of live weight while six other rams of each breeds were also slaughtered at 40 kg of live weight. Before the rams were slaughtered, they were fattened using pellet ration which was formulated according to NRC for fattening 10 kg of lamb, containing 73.3% TDN and 16% protein in dry matter bases. Feed and water were given ad libitum. Rams were slaughtered and dissected when they reached their assigned slaughter weight (32.5 and 40 kg. The results showed that both breeds had high percentage of carcass (53%-55% with no significantly different between breeds (P>0.05 either were slaughtered at 32.5 kg or 40 kg. However, carcass of Priangan rams had significantly more muscle, but less fat than Javanese Fat-tailed rams at mature live weight (P0.05 except for head, tail and testes (P<0.01 and liver (P<0.05. The edible and inedible portion of non-carcass ranged from 30%-32% and 12%-15% of slaughter weight, respectively.

  7. Relationships between carcass characteristics, meat quality, age and sex of free-ranging Alaskan reindeer: a pilot study

    OpenAIRE

    Renecker, Teresa A.; Lyle A. Renecker; Mallory, Frank F.

    2005-01-01

    Twenty-four reindeer (Rangifer tarandus) carcasses from male and female animals that ranged in age from calves to adults were purchased from Bering Sea Reindeer Products (BSRP), Nunivak Island, Alaska, USA. Preslaughter and abattoir procedures were observed and evaluated. Carcasses were split in half, weighed, and broke into wholesale primal cuts of chuck, rib, loin, and hindquarter. Each primal cut was weighed, boxed, and frozen. Each half carcass of primal cuts was later dissected into lean...

  8. Live weight and sex effects on carcass and meat quality of "Borrego terrincho-PDO" suckling lambs

    OpenAIRE

    Santos, Virgínia; Silva, Severiano; Mena, Elizabete; Azevedo, Jorge Manuel Teixeira de

    2007-01-01

    Fifty seven suckling lambs (28 males and 29 females) of the Churra da Terra Quente breed were used to evaluate the effects of live weight and sex on carcass composition and meat quality traits. Lambs were slaughtered at three weight classes ( 11 kg) according to "Borrego Terrincho-PDO" specifications. The left sides of the carcasses were totally dissected. The longissimus thoracis and lumborum muscle was used for meat quality determination. Dressing proportion and carcass...

  9. [Evaluation of common commercial systems for the identification of yeast isolates in microbiology laboratories: a multicenter study].

    Science.gov (United States)

    Karabıçak, Nilgün; Uludağ Altun, Hatice; Karatuna, Onur; Hazırolan, Gülşen; Aksu, Neriman; Adiloğlu, Ali; Akyar, Işın

    2015-04-01

    Accurate and rapid identification of yeast isolates have become important in recent years for not only antifungal susceptibility testing due to the species-specific clinical resistance breakpoints but also early initiation of appropriate antifungal therapy. In clinical microbiology laboratories species identification of yeasts is often performed with several commercial systems based on biochemical properties and rarely according to the physiological and morphological characteristics. The aim of this study was to compare the two common commercial systems, VITEK 2 YST ID Card (Vitek; bioMérieux, France) and API 20C AUX (API; bioMérieux, France) with conventional mycological methods. A total of 473 clinical yeast strains isolated from clinical specimens in different university and training/research hospitals and identified by Vitek system were included in the study. The isolates were re-identified with API and conventional methods including morphological identification in the Mycology Reference Laboratory of the Public Health Institute of Turkey. Candida dubliniensis MYA 583, Candida krusei ATCC 6258, Candida parapsilosis ATCC 22019, Candida albicans ATCC 10231 and Cryptococcus neoformans ATCC 32268 were used as quality control strains and those standard strains were studied consecutively 10 days with both of the methods. The results of identification by Vitek and API were compared with the results of conventional methods for those 473 yeast isolates [6 genus (Candida, Cryptococcus, Blastoshizomyces, Rhodotorula, Saccharomyces, Trichosporon), 17 species (5 common and 12 rarely isolated)]. The performances of the systems were better (Vitek: 95%; API: 96%) for the commonly detected species (C.albicans, C.parapsilosis, C.glabrata, C.tropicalis and C.krusei) than those for rarely detected species (Vitek: 78.4%; API: 71.6%) (p= 0.155). Misidentification or unidentification were mostly detected for C.parapsilosis (Vitek: 6/87; API: 7/87) and C.glabrata (Vitek: 9/104; API

  10. Common factors method to predict the carcass composition tissue in kid goats

    Directory of Open Access Journals (Sweden)

    Helen Fernanda Barros Gomes

    2013-03-01

    Full Text Available The objective of this work was to analyze the interrelations among weights and carcass measures of the longissimus lumborum muscle thickness and area, and of sternum tissue thickness, measured directly on carcass and by ultrasound scan. Measures were taken on live animals and after slaughter to develop models of multiple linear regression, to estimate the composition of shoulder blade, from selected variables in 89 kids of both genders and five breed groups, raised in feedlot system. The variables considered relevant and not redundant on the information they carry, for the common factor analysis, were used in the carcass composition estimate development models. The presuppositions of linear regression models relative to residues were evaluated, the estimated residues were subjected to analysis of variance and the means were compared by the Student t test. Based in these results, the group of 32 initial variables could be reduced to four variables: hot carcass weight, rump perimeter, leg length and tissue height at the fourth sternum bone. The analysis of common factors was shown as an effective technique to study the interrelations among the independent variables. The measures of carcass dimension, alone, did not add any information to hot carcass weight. The carcass muscle weight can be estimated with high precision from simple models, without the need for information related to gender and breed, and they could be built based on carcass weight, which makes it easy to be applied. The fat and bones estimate models were not as accurate.

  11. Characterization of biological types of cattle (Cycle VII): Carcass, yield, and longissimus palatability traits.

    Science.gov (United States)

    Wheeler, T L; Cundiff, L V; Shackelford, S D; Koohmaraie, M

    2005-01-01

    The objective of this experiment was to provide a current evaluation of the seven most prominent beef breeds in the United States and to determine the relative changes that have occurred in these breeds since they were evaluated with samples of sires born 25 to 30 yr earlier. Carcass (n = 649), yield (n = 569), and longissimus thoracis palatability (n = 569) traits from F(1) steers obtained from mating Hereford, Angus, and MARC III cows to Hereford (H), Angus (A), Red Angus (RA), Charolais (C), Limousin (L), Simmental (S), or Gelbvieh (G) sires were compared. Data were adjusted to constant age (445 d), carcass weight (363 kg), fat thickness (1.1 cm), fat trim percent (25%), and marbling (Small(35)) endpoints. For Warner-Bratzler shear force and trained sensory panel traits, data were obtained on LM from steaks stored at 2 degrees C for 14 d postmortem. The following comparisons were from the age-constant endpoint. Carcasses from L-, G-, and H-sired steers (361, 363, and 364 kg, respectively) were lighter (P carcasses from steers from all other sire breeds. Adjusted fat thickness for carcasses from A-, RA-, and H-sired steers (1.5, 1.4, and 1.3 cm, respectively) was higher (P carcasses from steers from all other sire breeds (0.9 cm). Longissimus muscle areas were largest (P carcasses from L-, C-, S-, and G-sired steers (89.9, 88.7, 87.6, and 86.5 cm(2), respectively) and smallest for carcasses from H- and RA-sired steers (79.5 and 78.4 cm(2)). A greater (P carcasses from RA- and A-sired steers graded USDA Choice (90 and 88%, respectively) than from carcasses from other sire breeds (57 to 66%). Carcass yield of boneless, totally trimmed retail product was least (P carcasses of A-sired steers (4.0 kg) had lower (P carcasses of G- and C-sired steers (4.5 to 4.3 kg, respectively). Trained sensory panel tenderness and beef flavor intensity ratings for LM did not differ (P carcasses than did British breeds (H, A, and RA), with less marbling than A or RA, although British

  12. European journals on microbiology.

    Science.gov (United States)

    Ronda, C; Vázquez, M

    1997-12-01

    A survey on the scientific journals dealing with microbiology published in Europe has been carried out. Eighteen European countries publish microbiological journals with the United Kingdom. Netherlands and Germany leading in number of journals on this specialty. Most of the European journals on microbiology are published bimonthly (27%), and English is the most common language used (54%). Most of these journals (86%) are included in some database, but only 36 (25%) are indexed in the six databases studied. Out of the 146 journals registered, 71 (49%), published in 11 European countries, are included in the 1995 Journal Citation Reports (ISI, Philadelphia).

  13. Effects of Rice Bran, Flax Seed, and Sunflower Seed on Growth Performance, Carcass Characteristics, Fatty Acid Composition, Free Amino Acid and Peptide Contents, and Sensory Evaluations of Native Korean Cattle (Hanwoo).

    Science.gov (United States)

    Choi, Chang Bon; Kwon, Hana; Kim, Sung Il; Yang, Un Mok; Lee, Ju Hwan; Park, Eun Kyu

    2016-02-01

    This study was conducted to evaluate the effect of dietary supplementation with rice bran, flax seed, or sunflower seed to finishing native Korean cattle (Hanwoo) on growth performances, carcass characteristics, fatty acid composition, free amino acid and peptide contents, and sensory evaluations of Longissimus muscle (LM). A total of 39 Hanwoo steers (average age of 22.2 mo and average body weight (BW) of 552.2 kg) were randomly divided into Control, rice bran (RB), flax seed (FS), or Sunflower seed (SS) groups. The steers were group fed for 273 d until they reached an average age of 31.2 mo. Final BW was 768.2, 785.8, 786.2, and 789.0 kg, and average daily gain was 0.79, 0.85, 0.82, and 0.84 kg for the Control, RS, FS, and SS groups, respectively (p>0.05). Fat thickness of the FS group (19.8 mm) was greater (p0.05) scores for flavor, umami, and overall palatability in sensory evaluations. In conclusion, supplementation of flax seed to diets of finishing Hanwoo steers improved sensory evaluations which might have been caused by increases in flavor related amino acids such as methionine, glutamic acid and α-AAA and peptides, anserine and carnosine, and their complex reactions. PMID:26732444

  14. Carcass and non-carcass characteristics of sheep fed on cassava (Manihot pseudoglaziovii Pax & K. Hoffm.

    Directory of Open Access Journals (Sweden)

    Michel V Maciel

    2015-09-01

    Full Text Available Sheep production systems installed in the semi-arid region of Brazil depend on the forage support the 'caatinga' biome. This study aimed at evaluating the substitution of hybrid 'Tifton 85' (Cynodon spp. by cassava (Manihotpseudoglaziovii Pax & K. Hoffm. hay or silage on the components of sheep's' body weight. Twenty-four animals, with no defined breed, were used for the study, with an initial body weight of 19.77 ± 1.95 kg and an average age of 6-mo, being divided into three treatments ('Tifton 85' hay, cassava silage, and cassava hay. The animals were slaughtered at 56 d and all the body parts of the animals were weighed. Data were subjected to ANOVA and mean comparison test (P = 0.05. Means were superior (P 0.05 for body weight at slaughter and cold carcass weight, which had means of 28.10 and 12.38 kg, respectively. The hot carcass and leg yields showed values of 58% and 34%, respectively, and were not influenced (P > 0.05 by different forages. The constituents that were not components of the carcass, organs, offal, and by-products were not affected by the replacement of 'Tifton 85' hay by cassava hay or silage. Cassava hay or silage can replace 'Tifton 85' hay for feeding sheep in complete diets without compromising their body components' yields and weights.

  15. Avaliação microbiológica de dietas enterais manipuladas em um hospital Microbiologic evaluation of enteral diets done in hospital

    Directory of Open Access Journals (Sweden)

    Ana Raquel da Costa Lima

    2005-01-01

    Full Text Available OBJETIVO: Avaliar a qualidade microbiológica de dietas enterais em sistema aberto manipuladas em um hospital especializado em oncologia na cidade de Natal-RN. MÉTODOS: Foram analisadas 20 amostras de dietas enterais industrializadas em sistema aberto. Os microrganismos pesquisados foram: Bacillus cereus, coliformes totais, Escherichia coli, Salmonella sp, Clostridium sulfito redutor, bactérias mesófilas aeróbias e Staphylococcus coagulase positiva. A metodologia empregada para realização das análises microbiológicas foi a preconizada pelo Food and Drug Administration - FDA. RESULTADOS: Foi evidenciada a contaminação por coliformes totais, Escherichia coli e bactérias mesófilas aeróbias em 25%, 10% e 20% das amostras analisadas, respectivamente. Não foi identificada a presença de Bacillus cereus, Salmonella sp, Clostridium sulfito redutor, e Staphylococcus coagulase positiva em nenhuma das amostras analisadas. Conclusão: Os resultados obtidos mostram que as dietas enterais encontram-se em condições higiênico-sanitárias insatisfatórias, evidenciando a necessidade de um maior controle na manipulação das mesmas.PURPOSE: To evaluate the microbiological quality of open enteral feeding system manipulated in a specialized hospital in oncology in the city of Natal-RN. METHODS: 20 samples of industrialized enteral feeding in open system had been analyzed. The searched microrganisms had been: Bacillus cereus, total coliforms, Escherichia coli, Salmonella sp, Clostridium sulfite reducer, mesophilic aerobic bacteria and coagulase positive Staphylococci. The methodology used for accomplishment of the microbiological analyses was the praised one for the Food and Drug Administration - FDA. RESULTS: It was evidenced total coliforms, Escherichia coli and mesophilic aerobic bacteria contamination in 25%, 10% and 20% of the analyzed samples, respectively. It was not identified the presence of Bacillus cereus, Salmonella sp, Clostridium

  16. Evaluation of Chemical and Microbiological Quality in 21 Brands of Iranian Bottled Drinking Waters in 2012: A Comparison Study on Label and Real Contents

    Directory of Open Access Journals (Sweden)

    M. Moazeni

    2013-01-01

    Full Text Available The purpose of this study was to evaluate and compare chemical and microbiological quality of the 21 Iranian bottled drinking waters reported on manufacturer's labeling and standards in 2012. Samples were analyzed for chemical properties K+, F−, SO4 2−, Cl−, Mg2+, Ca2+, and pH. Total and fecal coliform and heterotrophic plate counts of selected samples were analyzed by MPN and HPC tests, respectively, for three months. Finally, the labeled and real contents of the samples were compared. Potassium and sulfate ions about 43 and 52 percent of studied sample contents had values higher than label amounts, respectively. Ca2+, Cl− ions, and pH were about 71, 48, and 67 percent, respectively, less than label values. Total and fecal coliforms had negative results. The mean concentrations and standard deviations for K+, Cl−, pH, Ca2+, Mg2+, SO4 2−, and HPC were 1.13 ± 1.06, 16.39 ± 31.97, 6.6 ± 0.7, 28.35 ± 10.34, 86.58 ± 33.21, 24.17 ± 17.30 mg/L, and 16855 ± 25603 cfu/mL, respectively. Thus, there is possibility of microorganisms’ growth in favorite conditions in bottled water. It was imperative to assess the public health risks in bottled water in Iran.

  17. Next Generation Microbiology Requirements

    Science.gov (United States)

    Ott, C. M.; Oubre, C. M.; Elliott, T. F.; Castro, V. A.; Pierson, D. L.

    2012-01-01

    As humans continue to explore deep into space, microorganisms will travel with them. The primary means to mitigate the risk of infectious disease are a combination of prudent spacecraft design and rigorous operational controls. The effectiveness of these methods are evaluated by microbiological monitoring of spacecraft, food, water, and the crew that is performed preflight, in-flight, and post-flight. Current NASA requirements associated with microbiological monitoring are based on culture-based methodology where microorganisms are grown on a semi-solid growth medium and enumerated. Subsequent identification of the organisms requires specialized labor and large equipment, which historically has been performed on Earth. Requirements that rely strictly on culture-based units limit the use of non-culture based monitoring technology. Specifically, the culture-based "measurement criteria" are Colony Forming Units (CFU, representing the growth of one microorganism at a single location on the agar medium) per a given volume, area, or sample size. As the CFU unit by definition is culture-based, these requirements limit alternative technologies for spaceflight applications. As spaceflight missions such as those to Mars extend further into space, culture-based technology will become difficult to implement due to the (a) limited shelf life of the culture media, (b) mass/volume necessary to carry these consumables, and (c) problems associated with the production of biohazardous material in the habitable volume of the spacecraft. In addition, an extensive amount of new knowledge has been obtained during the Space Shuttle, NASA-Mir, and International Space Station Programs, which gave direction for new or modified microbial control requirements for vehicle design and mission operations. The goal of this task is to develop and recommend a new set of requirements for vehicle design and mission operations, including microbiological monitoring, based upon "lessons learned" and new

  18. Correlation between Sensory Evaluation and the Chemical and Microbiological Composition of Yoghourt%酸奶的感官与理化特性和乳酸菌活菌数的相关性

    Institute of Scientific and Technical Information of China (English)

    李俊芳; 康灵芝

    2015-01-01

    在前期酸奶工艺优化的基础上,对酸奶进行感官评分和理化、微生物检测,对酸奶感官评分与理化、微生物指标之间的相关性进行研究。结果表明:黏度、酸度、乳酸菌活菌数和持水性与感官特性有极显著的相关性,由此提出以黏度、酸度和乳酸菌活菌数以及持水性4个指标来客观评价酸奶品质,为酸奶的研制提供理论依据。%On the basis of the previous research about the process optimization of yoghourt. The yoghourt was analyzed by the method of sensory evaluation and the chemical and microbiological composition , and the relationship between the sensory evaluation and the chemical and microbiological composition of yoghourt were explored. The viscosity, acidity, water holding capacity and viable count of lactobacillus of yoghourt were significantly correlated with their sensory scores.Therefore, the Viscosity, acidity, water holding capacity and viable count of lactobacillus were applied to evaluate the quality of yoghourt. objectively , which will provide theoretical basis for the application of The chemical and microbiological composition in the yoghourt quality evaluation.

  19. Microbiology: a dangerous profession?

    Science.gov (United States)

    Piqueras, Mercè

    2007-09-01

    The history of science contains many cases of researchers who have died because of their professional activity. In the field of microbiology, some have died or have come close to death from infection by agents that were the subject of their research (Table 1). Infections that had a lethal outcome were usually accidental. Sometimes, however, researchers inoculated themselves with the pathogen or did not take preventive measures against the potential pathogen because they wanted to prove their hypotheses--or disprove someone else's--regarding the origin of the infection. Here is an overview of several episodes in the history of microbiology since the mid nineteenth century involving researchers or workers in fields related to microbiology who have become infected. They are considered here in their historical context to provide insights into some of the pillars of modern microbiology--The giants on whose shoulders several generations of microbiologists have stood to see further.

  20. Salmonella radicidation of poultry carcasses

    NARCIS (Netherlands)

    Mulder, R.W.A.W.

    1982-01-01

    Validity of methodsExperiments were carried out In which it was assessed which Salmonella isolation method is the most productive one In the examination of broiler carcasses. Refrigerated, refrigerated and radiated (2.50 kGy), frozen and frozen and radiated (2.50 kGy) samples of broile

  1. Salmonella radicidation of poultry carcasses

    International Nuclear Information System (INIS)

    This thesis reports investigations using gamma-radiation to decontaminate poultry carcasses. The application to foods of doses of ionizing radiation sufficient to reduce the number of viable specific non-sporeforming pathogenic microorganisms so that none is detectable in the treated food by any standard method is termed radicidation. The doses used in this study were at such a level that no undesirable or unfavourable side-effects occurred. The effects of these doses were studied on salmonellae and other microorganisms present in, or associated with poultry carcasses and in liquid and on solid culture media as well. Decimal reduction (D10) values were estimated. These represent the dose (kGy) required to achieve a reduction in initial colony count from N0 to 0.1 N0. Together with the estimation of the numbers of Salmonella present per carcass the data were used to predict the effect of an ionizing radiation treatment of poultry. Data on the effect of ionizing radiation on the total microflora of poultry carcasses were also collected. (Auth.)

  2. Consumer acceptance and carcass quality

    Science.gov (United States)

    In commodity production systems, beef quality is designated based on the USDA grading criteria which take into account carcass marbling, maturity and yield. Producers are rewarded economically for beef quality grade (QG) of Choice versus Select although the price difference (spread) varies seasonal...

  3. Clinical microbiology informatics.

    Science.gov (United States)

    Rhoads, Daniel D; Sintchenko, Vitali; Rauch, Carol A; Pantanowitz, Liron

    2014-10-01

    The clinical microbiology laboratory has responsibilities ranging from characterizing the causative agent in a patient's infection to helping detect global disease outbreaks. All of these processes are increasingly becoming partnered more intimately with informatics. Effective application of informatics tools can increase the accuracy, timeliness, and completeness of microbiology testing while decreasing the laboratory workload, which can lead to optimized laboratory workflow and decreased costs. Informatics is poised to be increasingly relevant in clinical microbiology, with the advent of total laboratory automation, complex instrument interfaces, electronic health records, clinical decision support tools, and the clinical implementation of microbial genome sequencing. This review discusses the diverse informatics aspects that are relevant to the clinical microbiology laboratory, including the following: the microbiology laboratory information system, decision support tools, expert systems, instrument interfaces, total laboratory automation, telemicrobiology, automated image analysis, nucleic acid sequence databases, electronic reporting of infectious agents to public health agencies, and disease outbreak surveillance. The breadth and utility of informatics tools used in clinical microbiology have made them indispensable to contemporary clinical and laboratory practice. Continued advances in technology and development of these informatics tools will further improve patient and public health care in the future.

  4. Safety Evaluation of Sous Vide-Processed Products with Respect to Nonproteolytic Clostridium botulinum by Use of Challenge Studies and Predictive Microbiological Models

    Science.gov (United States)

    Hyytiä-Trees, Eija; Skyttä, Eija; Mokkila, Mirja; Kinnunen, Arvo; Lindström, Miia; Lähteenmäki, Liisa; Ahvenainen, Raija; Korkeala, Hannu

    2000-01-01

    Sixteen different types of sous vide-processed products were evaluated for safety with respect to nonproteolytic group II Clostridium botulinum by using challenge tests with low (2.0-log-CFU/kg) and high (5.3-log-CFU/kg) inocula and two currently available predictive microbiological models, Food MicroModel (FMM) and Pathogen Modeling Program (PMP). After thermal processing, the products were stored at 4 and 8°C and examined for the presence of botulinal spores and neurotoxin on the sell-by date and 7 days after the sell-by date. Most of the thermal processes were found to be inadequate for eliminating spores, even in low-inoculum samples. Only 2 of the 16 products were found to be negative for botulinal spores and neurotoxin at both sampling times. Two products at the high inoculum level showed toxigenesis during storage at 8°C, one of them at the sell-by date. The predictions generated by both the FMM thermal death model and the FMM and PMP growth models were found to be inconsistent with the observed results in a majority of the challenges. The inaccurate predictions were caused by the limited number and range of the controlling factors in the models. Based on this study, it was concluded that the safety of sous vide products needs to be carefully evaluated product by product. Time-temperature combinations used in thermal treatments should be reevaluated to increase the efficiency of processing, and the use of additional antibotulinal hurdles, such as biopreservatives, should be assessed. PMID:10618228

  5. Safety evaluation of sous vide-processed products with respect to nonproteolytic Clostridium botulinum by use of challenge studies and predictive microbiological models.

    Science.gov (United States)

    Hyytiä-Trees, E; Skyttä, E; Mokkila, M; Kinnunen, A; Lindström, M; Lähteenmäki, L; Ahvenainen, R; Korkeala, H

    2000-01-01

    Sixteen different types of sous vide-processed products were evaluated for safety with respect to nonproteolytic group II Clostridium botulinum by using challenge tests with low (2. 0-log-CFU/kg) and high (5.3-log-CFU/kg) inocula and two currently available predictive microbiological models, Food MicroModel (FMM) and Pathogen Modeling Program (PMP). After thermal processing, the products were stored at 4 and 8 degrees C and examined for the presence of botulinal spores and neurotoxin on the sell-by date and 7 days after the sell-by date. Most of the thermal processes were found to be inadequate for eliminating spores, even in low-inoculum samples. Only 2 of the 16 products were found to be negative for botulinal spores and neurotoxin at both sampling times. Two products at the high inoculum level showed toxigenesis during storage at 8 degrees C, one of them at the sell-by date. The predictions generated by both the FMM thermal death model and the FMM and PMP growth models were found to be inconsistent with the observed results in a majority of the challenges. The inaccurate predictions were caused by the limited number and range of the controlling factors in the models. Based on this study, it was concluded that the safety of sous vide products needs to be carefully evaluated product by product. Time-temperature combinations used in thermal treatments should be reevaluated to increase the efficiency of processing, and the use of additional antibotulinal hurdles, such as biopreservatives, should be assessed. PMID:10618228

  6. IDENTIFICATIONS OF CARCASS CHARACTERISTIC FOR ESTIMATING THE COMPOSITION OF BEEF CARCASS

    OpenAIRE

    H. Hafid; R.E. Gurnadi; R. Priyanto; A. Saefuddin

    2014-01-01

    The research aimed to identify carcass characteristic that can be used for estimating composition ofbeef carcass. It was used 165 Brahman crossbred cattle in this research. Carcass characteristics wereweight of a half cold carcass (WC) ranged from 96 to 151 kg, loin eye area (LEA) ranged from 22.09 to304.8 mm2, 12th rib fat thickness (FT12) ranged from 0.80 to 2.90 mm, meat ranged from 53.55 to 90.10kg and carcass fat ranged from 5.54 to 39.72 kg. Result showed that a half weight cold carcass...

  7. Prediction of Carcass Composition Using Carcass Grading Traits in Hanwoo Steers.

    Science.gov (United States)

    Lee, Jooyoung; Won, Seunggun; Lee, Jeongkoo; Kim, Jongbok

    2016-09-01

    The prediction of carcass composition in Hanwoo steers is very important for value-based marketing, and the improvement of prediction accuracy and precision can be achieved through the analyses of independent variables using a prediction equation with a sufficient dataset. The present study was conducted to develop a prediction equation for Hanwoo carcass composition for which data was collected from 7,907 Hanwoo steers raised at a private farm in Gangwon Province, South Korea, and slaughtered in the period between January 2009 and September 2014. Carcass traits such as carcass weight (CWT), back fat thickness (BFT), eye-muscle area (EMA), and marbling score (MAR) were used as independent variables for the development of a prediction equation for carcass composition, such as retail cut weight and percentage (RC, and %RC, respectively), trimmed fat weight and percentage (FAT, and %FAT, respectively), and separated bone weight and percentage (BONE, and %BONE), and its feasibility for practical use was evaluated using the estimated retail yield percentage (ELP) currently used in Korea. The equations were functions of all the variables, and the significance was estimated via stepwise regression analyses. Further, the model equations were verified by means of the residual standard deviation and the coefficient of determination (R(2)) between the predicted and observed values. As the results of stepwise analyses, CWT was the most important single variable in the equation for RC and FAT, and BFT was the most important variable for the equation of %RC and %FAT. The precision and accuracy of three variable equation consisting CWT, BFT, and EMA were very similar to those of four variable equation that included all for independent variables (CWT, BFT, EMA, and MAR) in RC and FAT, while the three variable equations provided a more accurate prediction for %RC. Consequently, the three-variable equation might be more appropriate for practical use than the four-variable equation

  8. Teaching microbiological food safety through case studies

    Directory of Open Access Journals (Sweden)

    Florence Dubois-Brissonnet

    2015-10-01

    Full Text Available Higher education students usually ask for more training based on case studies. This was addressed by designing a specific food safety module (24 hours in which students were shown how to predict microbiological risks in food products i.e. they were asked to determine product shelf-life according to product formulation, preservation methods and consumption habits using predictive microbiology tools. Working groups of four students first identified the main microbiological hazards associated with a specific product. To perform this task, they were given several documents including guides for good hygiene practices, reviews on microbiological hazards in the food sector, flow sheets, etc…  After three-hours of work, the working groups prepared and gave an oral presentation in front of their classmates and professors. This raised comments and discussion that allowed students to adjust their conclusions before beginning the next step of their work. This second step consisted in the evaluation of the safety risk associated with the two major microbiological hazards of the product studied, using predictive microbiology. Students then attended a general lecture on the different tools of predictive microbiology and tutorials (6 hours that made them familiar with the modelling of bacterial growth or inactivation. They applied these tools (9 hours to predict the shelf-life of the studied product according to various scenarios of preservation (refrigeration, water activity, concentration of salt or acid, modified atmosphere, etc… and/or consumption procedures (cooking. The module was concluded by oral presentations of each working group and included student evaluation (3 hours.

  9. Biochemical and microbiological evaluation of the water samples collected from different areas of district Kohat and Mohamand Agency, Pakistan.

    Directory of Open Access Journals (Sweden)

    Ayaz Ali

    2013-09-01

    Full Text Available Contamination of drinking water sources mainly due to microorganisms is the major problem in many areas of Pakistan. Pakistan is also facing the problem of contamination of drinking water which greatly affects human health and quality of life. The most important component of human beings for living is water. Therefore, it is important to analyze drinking water quality mostly in developing countries as the local people are mostly unaware of the water pollution. In this study, twenty three samples of water were analyzed during a 3-month period from the well and lake water supplies of different areas of Kohat and Mohamand Agency. The bacteriological evaluation was done and several tests were performed such as Total Plate Count, Coliform, Feacal coliform, Escherichia coli (E. coli and Biochemical test. In this study, thirteen samples were in the normal range and 10 samples were out of safety ranges fixed by World Health Organization (WHO. The water which was not fit for drinking can be a consistent risk of the infectious diseases and continuous assessment and purification strategies should be developed in these areas to reduce the microbial contamination. The proper training by the local public authorities is required to educate the local community about water pollution, their causes and preventive measures in order to improve the health status of the people in the regions.

  10. Quality characteristics and microbiological safety evaluation of oils extracted from gamma irradiated almond (Prunus dulcis Mill.) seeds

    Energy Technology Data Exchange (ETDEWEB)

    Bhatti, I. A.; Iqbal, M.; Anwar, F.; Shanid, S. A.; Shahid, M.

    2013-05-01

    The physicochemical attributes and microbial decontamination of oils extracted from gamma-irradiated almond (Mission and Price varieties) seeds, to the absorbed doses of 2-10 kGy, have been evaluated. Gamma irradiation exerted no considerable effect on the proximate seed composition. The physicochemical properties such as density and refractive index of the oils, extracted from gammairradieted seeds, were almost unaffected; the iodine value decreased while saponification value, unsaponifiable matter and free fatty acids increased. The oxidative status and tocopherol content of almond oils were negatively affected while the fatty acid profile slightly changed due to irradiation stress. Interestingly, these effects on the oil quality attributes were more pronounced at higher irradiation doses (> 6 kGy). Besides, microbial contamination was completely eliminated in the oils irradiated to an absorbed dose of 6.0 kGy. It could be concluded from the present findings that irradiation has a considerably positive or negative effect on some attributes of the almond oil. Therefore, an appropriate magnitude of gamma irradiation should be exercised to treat almond seeds in order to retain maximum nutritive benefits. (Author) 52 refs.

  11. [The clinical and microbiological evaluation of the efficacy of oral irrigation on the periodontal tissues of patients wearing fixed orthodontic appliances].

    Science.gov (United States)

    Di Murro, C; Paolantonio, M; Petti, S; Tomassini, E; Festa, F; Grippaudo, C; Sbolgi, S

    1992-11-01

    The aim of the present study has been the evaluation of the effectiveness of oral irrigation with or without toothbrushing and dental flossing, in individuals treated with fixed orthodontic appliances, on controlling the development of dental plaque and, hence, of gingivitis. Eight individuals with a good general and oral status have been chosen. Before the experimental period, they received instructions about oral hygiene with toothbrushing and dental flossing and then they have been monitored to verify they were doing well. At the time T0, the upper tooth have been banded, three Periodontal Indexes (Plaque Index according to Silness and Loe, Modified Gingival Index according to Lobene and Gingival Bleeding Index according to Ainamo and Bay) registered and subgingival plaque samples from the premolars' gingival sulcus collected in order to point out the total anaerobes bacterial counts, the rates of motile bacteria, spirochetes, Gram positive and Gram negative cocci and bacteria, by means of optical and dark field microscopy and of cultural methods. For their oral hygiene, the patients had to use, in the right side toothbrush and dental floss (Control 1), in the left side the oral irrigator alone (Test 1). One month later (time T1), the lower teeth have been banded, too. In the right side the patients had to use toothbrush and dental floss (Control 2), while in the left one they used the same devices as Control 2 plus the oral irrigator (Test 2). At the time T1 Periodontal Indexes and Microbiological analyses have been extended to all the four quadrants.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:1294872

  12. Validation of a same-day real-time PCR method for screening of meat and carcass swabs for Salmonella

    DEFF Research Database (Denmark)

    Löfström, Charlotta; Krause, Michael; Josefsen, Mathilde Hartmann;

    2009-01-01

    -commercial real-time PCR method for detection of Salmonella in meat and carcass swabs. Results: The comparative trial was performed against a reference method (NMKL-71:5, 1999) using artificially and naturally contaminated samples (60 minced veal and pork meat samples, 60 poultry neck-skins, and 120 pig carcass...... contaminated with Salmonella. There was no significant difference in the results obtained by the two methods. Conclusion: The real-time PCR method for detection of Salmonella in meat and carcass swabs was validated in comparative and collaborative trials according to NordVal recommendations. The PCR method...... of the published PCR methods for Salmonella have been validated in collaborative studies. This study describes a validation including comparative and collaborative trials, based on the recommendations from the Nordic organization for validation of alternative microbiological methods (NordVal) of a same-day, non...

  13. Carcass and meat quality traits in Nellore and F1 Nellore-Araguaia crosses.

    Science.gov (United States)

    Costa, N V; Aboujaoude, C; Vieira, G S; Paiva, V V; Moraes Neto, R A; Gondim, V S; Alves, L R; Torres, M C L; Antunes, R C

    2015-05-22

    We evaluated and compared carcass traits and meat quality in Nellore cattle and F1 crosses between Nellore and Araguaia, where 17 individuals were from the Nellore group and 19 were ½ Nellore and ½ Araguaia crosses. All animals belonged to the same birth season and were raised in pasture systems under the same nutritional, environmental, and management conditions. When the animals reached slaughter weight, they were taken to an industrial slaughterhouse where food was not provided for 24 h (free access to water); they were then stunned, bled, the leather was removed, and they were eviscerated. The carcasses were weighed (hot weight), kept in chilled storage for approximately 24 h at 4°C, and weighed again to obtain the chilled carcass weight. Carcass yield, carcass length, carcass width, leg length, thigh perimeter, loin eye area (LEA), retail cuts, cooling loss, pH, fat depth, marbling rate, intramuscular fat, color, and shear force were analyzed and sensory analysis of the meat was conducted. Significant differences (P meat's sensory characteristics, but contributed to an improvement in carcass traits, providing an alternative for farmers that aim for good meat quality, with a higher meat percentage.

  14. Quality characteristics and microbiological safety evaluation of oils extracted from gamma irradiated almond (Prunus dulcis Mill. seeds

    Directory of Open Access Journals (Sweden)

    Shahid, S. A.

    2013-03-01

    Full Text Available The physicochemical attributes and microbial decontamination of oils extracted from gamma-irradiated almond (Mission and Price varieties seeds, to the absorbed doses of 2-10 kGy, have been evaluated. Gamma irradiation exerted no considerable effect on the proximate seed composition. The physicochemical properties such as density and refractive index of the oils, extracted from gammairradieted seeds, were almost unaffected; the iodine value decreased while saponification value, unsaponifiable matter and free fatty acids increased. The oxidative status and tocopherol content of almond oils were negatively affected while the fatty acid profile slightly changed due to irradiation stress. Interestingly, these effects on the oil quality attributes were more pronounced at higher irradiation doses (> 6 kGy. Besides, microbial contamination was completely eliminated in the oils irradiated to an absorbed dose of 6.0 kGy. It could be concluded from the present findings that irradiation has a considerably positive or negative effect on some attributes of the almond oil. Therefore, an appropriate magnitude of gamma irradiation should be exercised to treat almond seeds in order to retain maximum nutritive benefits.Se ha evaluado las caracteristicas fisico-químicas y la descontaminación microbiana de aceites extraídos de semillas de almendras (variedades Misión y Price gamma-irradiadas a dosis absorbidas de 2-10 kGy. La radiación gamma no ejerce ningún efecto considerable en la composición próximal de las semillas. Las características fisico-químicas tales como la densidad y el índice de refracción de los aceites, extraídos a partir de semillas gamma-irradiadas, permanecieron casi sin afectar; el índice de yodo disminuye mientras que el valor de saponificación, la materia insaponificable y los ácidos grasos libres aumentan. El estado oxidativo y el contenido de tocoferoles de los aceites de almendra se vieron afectados negativamente, mientras

  15. Single nucleotide polymorphisms associated with carcass traits in a population of Brahman and Brahman-influenced steers.

    Science.gov (United States)

    Royer, A M; Shivers, C; Riley, D G; Elzo, M A; Garcia, M D

    2016-01-01

    Brahman cattle are important in tropical regions due to their ability to tolerate excessive heat and parasites. However, Brahman cattle exhibit lower carcass quality characteristics when compared to Bos taurus breeds. The objective of this study was to evaluate potential associations between single nucleotide polymorphisms (SNPs) in six candidate genes for carcass quality and composition traits in a population of Brahman and Brahman-influenced steers. Steers were evaluated through the American Brahman Breeders Association carcass evaluation project in Gonzales, Texas. Carcass traits measured included hot carcass weight, ribeye area, marbling score, yield grade, quality grade, dressing percent, and Warner-Bratzler shear force score. Six previously described candidate genes were chosen for SNP analysis based on their previous association with growth and carcass traits. Candidate genes utilized in the current study included calpastatin (CAST), calpain (CAPN3), thyroglobulin (TG), growth hormone, insulin growth factor 1, and adiponectin. Six unique SNPs from three candidate genes (TG, CAST, and CAPN3) were significantly associated (P carcass quality traits (marbling score and quality grade). A genotypic effect was observed for all significant SNPs, with differing levels of performance observed for animals inheriting different genotypes. Although multiple SNPs in the current study were significantly (P carcass traits, they should be validated in larger populations prior to implementation in selection strategies.

  16. Single nucleotide polymorphisms associated with carcass traits in a population of Brahman and Brahman-influenced steers.

    Science.gov (United States)

    Royer, A M; Shivers, C; Riley, D G; Elzo, M A; Garcia, M D

    2016-06-21

    Brahman cattle are important in tropical regions due to their ability to tolerate excessive heat and parasites. However, Brahman cattle exhibit lower carcass quality characteristics when compared to Bos taurus breeds. The objective of this study was to evaluate potential associations between single nucleotide polymorphisms (SNPs) in six candidate genes for carcass quality and composition traits in a population of Brahman and Brahman-influenced steers. Steers were evaluated through the American Brahman Breeders Association carcass evaluation project in Gonzales, Texas. Carcass traits measured included hot carcass weight, ribeye area, marbling score, yield grade, quality grade, dressing percent, and Warner-Bratzler shear force score. Six previously described candidate genes were chosen for SNP analysis based on their previous association with growth and carcass traits. Candidate genes utilized in the current study included calpastatin (CAST), calpain (CAPN3), thyroglobulin (TG), growth hormone, insulin growth factor 1, and adiponectin. Six unique SNPs from three candidate genes (TG, CAST, and CAPN3) were significantly associated (P carcass quality traits (marbling score and quality grade). A genotypic effect was observed for all significant SNPs, with differing levels of performance observed for animals inheriting different genotypes. Although multiple SNPs in the current study were significantly (P carcass traits, they should be validated in larger populations prior to implementation in selection strategies.

  17. Salmonella Detection and Aerobic Colony Count in Deep-Frozen Carcasses of House Sparrow (Passer Domesticus) and Starling (Sturnus Vulgaris) Intended for Human Consumption

    Science.gov (United States)

    De Cesare, Alessandra; Braggio, Simonetta; Manfreda, Gerardo

    2014-01-01

    Wild birds are potential vehicles of zoonotic pathogen transmission to humans. The zoonotic concern increases for small wild birds like house sparrows (Passer domesticus) and starlings (Sturnus vulgaris) which are hunted in developing countries and commercialised in Italy for human consumption. From June to October 2011, 330 house sparrows and 140 starlings were hunted and slaughtered. Deep-frozen carcasses were transported to Italy and stored for 6-8 months at -18°C. Aerobic colony count and Salmonella detection in carcasses were assessed following standard microbiological methods (ISO 4833:2003 and ISO 6579:2004, respectively). Carcasses of house sparrows showed higher levels of aerobic bacteria in comparison to starling carcasses (5.7 vs 3.2 log10 CFU/g). Moreover, 7 out of 11 lots of carcasses of house sparrows were positive for Salmonella. Among the 18 isolates of Salmonella, 14 were S. Typhimurium, 2 were S. Enteritidis, and 2 were not distinguishable. All of them were susceptible to antibiotics. All tested carcasses of starling were Salmonella negative. Deep-freezing was not efficient as a decontamination technique on carcasses of house sparrows.

  18. Trends in the microbial contamination of bovine, ovine and swine carcasses in three small-scale abattoirs in central Italy: A four-year monitoring.

    Science.gov (United States)

    Petruzzelli, Annalisa; Osimani, Andrea; Pasquini, Marina; Clementi, Francesca; Vetrano, Vittorio; Paolini, Francesca; Foglini, Martina; Micci, Eleonora; Paoloni, Alberto; Tonucci, Franco

    2016-01-01

    The microbial contamination of animal carcasses with respect to the limits established by Regulation (EC) No. 2073/2005 was investigated. Bovine, ovine, and swine carcasses (n=536 samples) from three small-scale abattoirs were sampled using abrasive sponges and tested for aerobic colony counts (ACC) and Enterobacteriaceae in the period 2010-2013. Mean ACC values reached 1.96 log cfu/cm(2) on bovine carcasses and 2.27 log cfu/cm(2) on both swine and ovine carcasses; Enterobacteriaceae counts of 0.01, 0.20 and 0.27 log cfu/cm(2) were found for bovine, swine and ovine carcasses, respectively. Abattoir 1 showed the highest values of ACC; no differences among abattoirs were highlighted for Enterobacteriaceae. Compared with swine and ovine carcasses, bovine carcasses showed significantly lower means for both ACC and Enterobacteriaceae. The data collected indicated that the management of the three abattoirs met high quality standards, thereby proving that it is feasible to achieve good microbiological quality in abattoirs when adequate Good Hygiene Practices are applied.

  19. Deer carcass decomposition and potential scavenger exposure to chronic wasting disease

    Science.gov (United States)

    Jennelle, C.S.; Samuel, M.D.; Nolden, C.A.; Berkley, E.A.

    2009-01-01

    Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy afflicting the Cervidae family in North America, causing neurodegeneration and ultimately death. Although there are no reports of natural cross-species transmission of CWD to noncervids, infected deer carcasses pose a potential risk of CWD exposure for other animals. We placed 40 disease-free white-tailed deer (Odocoileus virginianus) carcasses and 10 gut piles in the CWD-affected area of Wisconsin (USA) from September to April in 2003 through 2005. We used photos from remotely operated cameras to characterize scavenger visitation and relative activity. To evaluate factors driving the rate of carcass removal (decomposition), we used KaplanMeier survival analysis and a generalized linear mixed model. We recorded 14 species of scavenging mammals (6 visiting species) and 14 species of scavenging birds (8 visiting species). Prominent scavengers included American crows (Corvus brachyrhynchos), raccoons (Procyon lotor), and Virginia opossums (Didelphis virginiana). We found no evidence that deer consumed conspecific remains, although they visited gut piles more often than carcasses relative to temporal availability in the environment. Domestic dogs, cats, and cows either scavenged or visited carcass sites, which could lead to human exposure to CWD. Deer carcasses persisted for 18 days to 101 days depending on the season and year, whereas gut piles lasted for 3 days. Habitat did not influence carcass decomposition, but mammalian and avian scavenger activity and higher temperatures were positively associated with faster removal. Infected deer carcasses or gut piles can serve as potential sources of CWD prions to a variety of scavengers. In areas where surveillance for CWD exposure is practical, management agencies should consider strategies for testing primary scavengers of deer carcass material.

  20. 基于灰AHP-白化权函数聚类评定法的多元精细化猪胴体分级方法研究%Study on multielement precise pig carcass grading methods based on grey AHP- whiten function clustering evaluation method

    Institute of Scientific and Technical Information of China (English)

    冷晟; 孙志超; 马万太

    2015-01-01

    提出的一种适用于自动化生猪屠宰线上的,多元、精细、智能化胴体肉品分级方法,创新性引入生猪养殖过程中饲料投喂与喂药信息,并采用适用于屠宰生产线上自动检测装置,快速有效地获取屠宰过程中胴体参数,依据生猪养殖信息、胴体特性和肉质特性这三类信息建立两阶段分级模型,采用灰色AHP-白化权函数聚类综合分级评定模型进行猪胴体肉品综合智能分级。使得猪胴肉品分级更加合理科学,适合中国生猪品种,易于推广,适用于现代化大规模自动化屠宰生产线。%A multiple indexes, refinement and intelligent pig carcass grading method had been pro⁃posed which could be adopted in modern automatic pig slaughtering line. The feed and medicine informa⁃tion of pigs breeding process was innovatively introduced. The automatic detection equipments which ap⁃plied to slaughtering line were used. The carcass parameters could be collected quickly and efficiently from slaughtering process. According to three kinds of information of breeding information, carcass char⁃acteristics and meat quality characteristics, the carcass grading model was set into two phases. AHP-Grey Whiten Function Clustering Evaluation Method was used to grade pig carcass quality. It made the carcass grading more reasonable. It is suitable for Chinese pig and easy to spread. It is suitable for large scale automation slaughtering production line.

  1. Carcass characteristics, meat quality, feeding behavior of Nelore heifers fed diets containing sunflower pie

    Directory of Open Access Journals (Sweden)

    Karine Regina Alves

    2016-05-01

    Full Text Available The aim was to evaluate the feeding behavior, carcass characteristics and meat quality of Nelore heifers fed sunflower pie replacing cottonseed meal. They were used 30 heifers with average initial weight of 274 kg ± 28 kg and average age of 24 months, confined for 76 days. The design was completely randomized with five treatments (0, 150, 300, 450 and 600 g kg-1 DM and six replicates per treatment. By completing 24 hours after slaughter, it evaluated muscle conformation and degree of finish made the longissimus dorsi at the 13th rib. A representative sample between the 10th and 12th ribs was collected for evaluation of the physical components of carcasses. As well as the other variables, the hot carcass weight, dressing percentage, carcass length and rib eye area were not influenced by different pie levels in feed. The mean percentage of muscle, bone and fat from the carcass were 53.45; 16.14 and 29.46%, respectively, similar among treatments. Therefore, the use of up to 600 g kg-1 of sunflower pie replacing cottonseed meal as the main protein source, did not affect the carcass characteristics and meat quality of Nelore heifers.

  2. Effect of Carcass Traits on Carcass Prices of Holstein Steers in Korea

    OpenAIRE

    Alam, M; Cho, K.H.; S. S. Lee; Choy, Y. H.; Kim, H S; Cho, C. I.; Choi, T. J.

    2013-01-01

    The present study investigated the contribution of carcass traits on carcass prices of Holstein steers in Korea. Phenotypic data consisted of 76,814 slaughtered Holsteins (1 to 6 yrs) from all over Korea. The means for live body weight at slaughter (BWT), chilled carcass weight (CWT), dressing percentage (DP), quantity grade index (QGI), eye muscle area (EMA), backfat thickness (BF) and marbling score (MS), carcass unit price (CUP), and carcass sell prices (CSP) were 729.0 kg, 414.2 kg, 56.79...

  3. Microbiology of ensiling

    Science.gov (United States)

    Recent advances in our understanding of silage microbiology are reviewed. The ability to extract microbial DNA from silages, amplify portions of DNA, and then separate those portions by the strains of microorganisms that have produced them has been at the core of the changes that have occurred recen...

  4. Making Microbiology Even Smaller!

    Science.gov (United States)

    Young, Linda Mull; Motz, Vicki Abrams

    2013-01-01

    We outline protocols for producing slant-minis (SLINIs) and mini-deeps (MEEPs) and examples of their use in simple microbiology experiments suitable for high school students. The principal benefits of these protocols are decreased cost associated with significantly reduced media use; easier, less expensive disposal of waste; and increased safety…

  5. Microbiology Made Relevant

    Science.gov (United States)

    Cronholm, Lois S.; Metz, Mildred C.

    1976-01-01

    Described are two hospital-based laboratory exercises which helped students perceive the relationship between the principles of microbiology and the practice of nursing. The exercises involved an environmental study focusing on problems of nosocomial infection and a study of patients hospitalized with infectious diseases. (Author/MS)

  6. Microbiology--Safety Considerations.

    Science.gov (United States)

    Hoffmann, Sheryl K.

    This paper discusses the risk assessment associated with microbiology instruction based on grade level, general control measures, appropriate activities for middle school and high school students, the preparation and sterilization of equipment, and safe handling techniques. Appended are instructions and figures on making wire loops and the…

  7. USDA yield grades and various carcass traits as predictors of carcass composition.

    Science.gov (United States)

    Lunt, D K; Smith, G C; Savell, J W; Murphey, C E; Carpenter, Z L; McKeith, F K; Johnson, D D

    1985-01-01

    Twenty-five carcasses from each of three breedtypes (Brahman, Angus and Brahman × Angus) were physically separated into fat, lean and bone. Several muscles from the round and the femur were used to derive equations to predict carcass composition and muscle-to-bone ratio. The femur (as a percentage of the carcass) was shown to predict percentage carcass bone with 90% accuracy. All of the muscles studied were highly related to total carcass lean but the percentage of carcass as M. biceps femoris was the best single muscle indicator of carcass lean of the muscles studied. More variation in carcass lean could be accounted for by a multiple regression equation, involving all four muscles studied, than by any single muscle. M. biceps femoris-to-femur ratio was found to predict carcass muscle-to-bone ratio with a high degree of accuracy. The USDA yield grades were found to be reliable indicators of carcass composition. A two-variable equation involving adjusted fat thickness and biceps femoris accounted for 88·6% of the variation (RSD = 1/·64) in percentage of carcass as separable lean.

  8. USDA yield grades and various carcass traits as predictors of carcass composition.

    Science.gov (United States)

    Lunt, D K; Smith, G C; Savell, J W; Murphey, C E; Carpenter, Z L; McKeith, F K; Johnson, D D

    1985-01-01

    Twenty-five carcasses from each of three breedtypes (Brahman, Angus and Brahman × Angus) were physically separated into fat, lean and bone. Several muscles from the round and the femur were used to derive equations to predict carcass composition and muscle-to-bone ratio. The femur (as a percentage of the carcass) was shown to predict percentage carcass bone with 90% accuracy. All of the muscles studied were highly related to total carcass lean but the percentage of carcass as M. biceps femoris was the best single muscle indicator of carcass lean of the muscles studied. More variation in carcass lean could be accounted for by a multiple regression equation, involving all four muscles studied, than by any single muscle. M. biceps femoris-to-femur ratio was found to predict carcass muscle-to-bone ratio with a high degree of accuracy. The USDA yield grades were found to be reliable indicators of carcass composition. A two-variable equation involving adjusted fat thickness and biceps femoris accounted for 88·6% of the variation (RSD = 1/·64) in percentage of carcass as separable lean. PMID:22055934

  9. Two-Center Collaborative Evaluation of Performance of the BD Phoenix Automated Microbiology System for Identification and Antimicrobial Susceptibility Testing of Gram-Negative Bacteria▿

    Science.gov (United States)

    Menozzi, Maria Grazia; Eigner, Ulrich; Covan, Silvia; Rossi, Sabina; Somenzi, Pietro; Dettori, Giuseppe; Chezzi, Carlo; Fahr, Anne-Marie

    2006-01-01

    The performance of the BD Phoenix Automated Microbiology System (BD Diagnostic Systems, Sparks, MD) was assessed for identification (ID) and antimicrobial susceptibility testing (AST) of the majority of clinically encountered bacterial isolates in a European collaborative two-center trial. A total of 494 bacterial isolates including various species of the Enterobacteriaceae and 110 nonfermentative gram-negative bacteria were investigated: of these, 385 were single patient isolates, and 109 were challenge strains tested at one center. The performance of the Phoenix extended-spectrum β-lactamase (ESBL) test was also evaluated for 203 strains of Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca included in the study. Forty-two antimicrobial drugs were tested, including members of the following drug classes: aminoglycosides, β-lactam antibiotics, β-lactam/β-lactamase inhibitors, carbapenems, cephems, monobactams, folate antagonists, quinolones, and others. Phoenix system ID results were compared to those of the laboratories' routine ID systems (API 20E and API CHE, ATB ID32E, ID32GN, and VITEK 2 [bioMérieux, Marcy l'Etoile, France]); Phoenix AST results were compared to those of frozen standard broth microdilution (SBM) panels according to NCCLS (now CLSI) guidelines (NCCLS document M100-S9, approved standard M7-A4). Discrepant results were repeated in duplicate. Concordant IDs of 98.4 and 99.1% were observed for the Enterobacteriaceae and the nonfermentative group, respectively. For AST results, the overall essential agreement was 94.2%; the category agreement was 97.3%; and the very major error rate, major error rate, and minor error rate were 1.6, 0.6, and 1.9%, respectively. In terms of ESBL detection, Phoenix results were 98.5% concordant with those of the reference system, with 98.0% sensitivity and 98.7% specificity. In conclusion, the Phoenix ID results showed high agreement with results of the systems to which they were being compared: the

  10. Two-center collaborative evaluation of performance of the BD phoenix automated microbiology system for identification and antimicrobial susceptibility testing of gram-negative bacteria.

    Science.gov (United States)

    Menozzi, Maria Grazia; Eigner, Ulrich; Covan, Silvia; Rossi, Sabina; Somenzi, Pietro; Dettori, Giuseppe; Chezzi, Carlo; Fahr, Anne-Marie

    2006-11-01

    The performance of the BD Phoenix Automated Microbiology System (BD Diagnostic Systems, Sparks, MD) was assessed for identification (ID) and antimicrobial susceptibility testing (AST) of the majority of clinically encountered bacterial isolates in a European collaborative two-center trial. A total of 494 bacterial isolates including various species of the Enterobacteriaceae and 110 nonfermentative gram-negative bacteria were investigated: of these, 385 were single patient isolates, and 109 were challenge strains tested at one center. The performance of the Phoenix extended-spectrum beta-lactamase (ESBL) test was also evaluated for 203 strains of Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca included in the study. Forty-two antimicrobial drugs were tested, including members of the following drug classes: aminoglycosides, beta-lactam antibiotics, beta-lactam/beta-lactamase inhibitors, carbapenems, cephems, monobactams, folate antagonists, quinolones, and others. Phoenix system ID results were compared to those of the laboratories' routine ID systems (API 20E and API CHE, ATB ID32E, ID32GN, and VITEK 2 [bioMérieux, Marcy l'Etoile, France]); Phoenix AST results were compared to those of frozen standard broth microdilution (SBM) panels according to NCCLS (now CLSI) guidelines (NCCLS document M100-S9, approved standard M7-A4). Discrepant results were repeated in duplicate. Concordant IDs of 98.4 and 99.1% were observed for the Enterobacteriaceae and the nonfermentative group, respectively. For AST results, the overall essential agreement was 94.2%; the category agreement was 97.3%; and the very major error rate, major error rate, and minor error rate were 1.6, 0.6, and 1.9%, respectively. In terms of ESBL detection, Phoenix results were 98.5% concordant with those of the reference system, with 98.0% sensitivity and 98.7% specificity. In conclusion, the Phoenix ID results showed high agreement with results of the systems to which they were being

  11. Influence of brahman-derivative breeds and Angus on carcass traits, physical composition, and palatability.

    Science.gov (United States)

    Bidner, T D; Wyattt, W E; Humes, P E; Franke, D E; Blouin, D C

    2002-08-01

    Steers were generated from Angus (A), Beefmaster (BM), Brangus (BA), Gelbray (GB), and Simbrah (SB) sires mated to cows of their breed and to Brahman x Hereford F1 cows (except A) to characterize their carcass traits, composition, and palatability. The 290 steers (48 A, 48 BM, 36 BA, 31 GB, and 46 SB) were slaughtered at an equal fatness end point as determined by real-time ultrasound and visual evaluation. Angus steers had lighter (P carcasses with a higher (P Brahman-derivative sired steers. The BM- and BA-sired steers had lighter (P carcasses, and smaller (P carcasses than the GB-sired steers. There were no differences in shear force for steaks aged for 3 d for any of the breed types, but with 10 d of aging, steaks from Angus steers were more tender, possibly indicating that steaks of Brahman-derivative breeds aged at a slower rate than those from Angus.

  12. Bacteriological quality of freshly processed broiler chickens as affected by carcass pretreatment and gamma irradiation

    International Nuclear Information System (INIS)

    Chicken carcasses dipped in whey fermented by Streptococcus thermophilus, lactic acid solution or water and irradiated at 2.5 kGy by 60Co were evaluated for bacteriological quality on day 1, day-3 and at 3-day intervals for an 18-day storage (4 degrees C) period. Unirradiated carcasses treated similarly were used as control. Gram negative bacteria, Yersinia and Campylobacter counts were significantly (p0.01) lower in irradiated samples, but no significant (p0.05) differences were observed ammong the dipping solutions. Salmonellae were completely eliminated in irradiated samples. Whey fermented by S. thermophilus reduced the proportion of Salmonella contaminated carcasses from 67% to 20%. As evidenced by the bacterial counts the shelf-life was found to be 15 days for irradiated carcasses compared to about 6 days for the unirradiated samples

  13. Microbiological aspects of microbiologically influenced corrosion

    International Nuclear Information System (INIS)

    Microbiologically influenced corrosion (MIC) is now recognized as commonly occurring in power plants as well as other industries. An Electric Power Research Institute (EPRI) sponsored survey and the case histories it contained implicated MIC in fire protection systems, heat exchangers, and the structural reliability and functioning of a variety of service water system components at nuclear power plants. A similar study covering various components of fossil-fired plants found that heat exchangers, piping and storage vessels were susceptible to MIC. A more recent publication sponsored by EPRI contained additional case histories covering MIC of carbon steels, stainless steels, copper alloys and high nickel alloys in a variety of components in nuclear power plants. In this paper the authors present field and laboratory experiences in MIC of various alloys and present a model for the pitting corrosion of carbon steel. In the case of other alloys, more speculative information is presented attempting to relate the microbes and their activities to the corrosion

  14. Investigating the impact of large carcass feeding on the behavior of captive Andean condors (Vultur gryphus) and its perception by zoo visitors.

    Science.gov (United States)

    Gaengler, Hannah; Clum, Nancy

    2015-01-01

    Naturalistic feeding methods, such as the provision of whole carcasses to zoo animals, are potentially controversial because zoo visitors might not approve of them. However, since several species of zoo animals feed from large carcasses in the wild, this food type could benefit their welfare in captivity compared to other less-natural food types. Scavengers in particular almost exclusively live on carcasses in nature; therefore, their welfare in captivity could significantly depend on the opportunity to express behaviors related to carcass feeding. In this study, we assessed the frequency of carcass feeding for vultures in North American zoos and investigated the effect of different food types on the behavior of zoo-housed Andean condors (Vultur gryphus). We also evaluated the opinion of North American zoo visitors about carcass feeding. Our results show that small whole carcasses (rats, rabbits) are part of the diet of vultures in most North American zoos, but large whole carcasses (ungulates) are rarely fed. Our behavioral study indicated that Andean condors appear to be more motivated to feed on more natural food types, which also seem to physically engage the birds more and occupy them longer. Most zoo visitors approved of carcass feeding for captive vultures over a range of prey animals, and the majority would also like to observe the vultures eat. Collectively, our results demonstrate that carcass feeding, particularly with larger prey, potentially enriches both zoo-housed vultures as well as the visitor experience.

  15. Investigating the impact of large carcass feeding on the behavior of captive Andean condors (Vultur gryphus) and its perception by zoo visitors.

    Science.gov (United States)

    Gaengler, Hannah; Clum, Nancy

    2015-01-01

    Naturalistic feeding methods, such as the provision of whole carcasses to zoo animals, are potentially controversial because zoo visitors might not approve of them. However, since several species of zoo animals feed from large carcasses in the wild, this food type could benefit their welfare in captivity compared to other less-natural food types. Scavengers in particular almost exclusively live on carcasses in nature; therefore, their welfare in captivity could significantly depend on the opportunity to express behaviors related to carcass feeding. In this study, we assessed the frequency of carcass feeding for vultures in North American zoos and investigated the effect of different food types on the behavior of zoo-housed Andean condors (Vultur gryphus). We also evaluated the opinion of North American zoo visitors about carcass feeding. Our results show that small whole carcasses (rats, rabbits) are part of the diet of vultures in most North American zoos, but large whole carcasses (ungulates) are rarely fed. Our behavioral study indicated that Andean condors appear to be more motivated to feed on more natural food types, which also seem to physically engage the birds more and occupy them longer. Most zoo visitors approved of carcass feeding for captive vultures over a range of prey animals, and the majority would also like to observe the vultures eat. Collectively, our results demonstrate that carcass feeding, particularly with larger prey, potentially enriches both zoo-housed vultures as well as the visitor experience. PMID:25653198

  16. Carcass characteristics of sheep fed diets with slow-release urea replacing conventional urea

    Directory of Open Access Journals (Sweden)

    Evanilton Moura Alves

    2014-07-01

    Full Text Available This study aimed to evaluate the effects of adding slow-release urea to replace conventional urea in the diet on carcass characteristics of feedlot sheep. We used 20 Santa Ines x SRD rams, with average body weight of 21.1±1.2 kgand average age of 120 days, distributed in a completely randomized design with 5 treatments. The replacement levels used as treatments were 0, 20, 40, 60, and 80%, composing diets of about 12% crude protein, with 50 % Tifton-85 hay and 50% concentrate. There was no influence of slow release urea on weight at slaughter (35.17 kg, and on hot (16.75 kg and cold (16.24 kg carcass weight, but the yield of these carcasses showed quadratic trend, revealing lower percentages at 48.5 and 47.63% replacement levels, respectively. The weights and yields of cuts did not change, except for the posterior arm, whose values showed a cubic trend. Objective measures of carcass, loin eye area, and subjective evaluations of conformation, finishing and marbling of carcasses were not affected. The subcutaneous fat thickness decreased linearly (4.25 to2.48 mm. The inclusion of slow release urea in the diet changes the yield and reduces subcutaneous fat, however, it does not influence other carcass characteristics.

  17. CARCASS AND MEAT CHARACTERISTICS OF CULL COWS SLAUGHTERED AT DISTINCT WEIGHTS AND DEGREE OF FATNESS – META-ANALYTICAL APPROACH

    Directory of Open Access Journals (Sweden)

    Leonel Da Silva Rodrigues

    2015-10-01

    Full Text Available The aim of this study was to carry out a meta-analysis of the carcass and meat characteristics of cull cows slaughtered at different weights and degree of fatness. The treatments were according to slaughter weight, (above 480.5 kg , Heavy, or below 480 kg, Light, of LW; and carcass degree of fatness (fat thickness between 6 and 10 mm, >6, or between 3 and 6 mm, <6. A completely random experimental design with a 2 x 2 factorial arrangement was used with two classes of slaughter weight and two classes of carcass degree of fatness. We evaluated papers about studies on cull cows carcass and meat, performed in Brazil between January 2000 and March 2013, available for access at SciELO and Capes search platforms. The papers were found through the combination of the following keywords: carcass, meat, bovine females, heifers and cows. The increase in cow’s slaughter weight did not affect hot carcass weight (252.3 versus 233.3 kg. There was less hot carcass yield (52.9 versus 56.9% and higher conformation (10.0 against 8.6 points. The highest degree of finishing positively influenced marbling (8.7 versus 5.4 points. The increase in slaughter weight produces better carcass conformation but does not affect meat quality; on the other hand, raising the finishing degree above 6mm changes the sensory characteristics of meat.

  18. Microbiology on Space Station Freedom

    Science.gov (United States)

    Pierson, Duane L. (Editor); Mcginnis, Michael R. (Editor); Mishra, S. K. (Editor); Wogan, Christine F. (Editor)

    1991-01-01

    This panel discussion convened in Houston, Texas, at the Lunar and Planetary Institute, on November 6 to 8, 1989, to review NASA's plans for microbiology on Space Station Freedom. A panel of distinguished scientists reviewed, validated, and recommended revisions to NASA's proposed acceptability standards for air, water, and internal surfaces on board Freedom. Also reviewed were the proposed microbiology capabilities and monitoring plan, disinfection procedures, waste management, and clinical issues. In the opinion of this advisory panel, ensuring the health of the Freedom's crews requires a strong goal-oriented research effort to determine the potential effects of microorganisms on the crewmembers and on the physical environment of the station. Because there are very few data addressing the fundamental question of how microgravity influences microbial function, the panel recommended establishing a ground-based microbial model of Freedom, with subsequent evaluation using in-flight shuttle data. Sampling techniques and standards will be affected by both technological advances in microgravity-compatible instrumentation, and by changes in the microbial population over the life of the station.

  19. Microbiological, physicochemical and sensory evaluation of cabbage and carrot minimally processed salad after radiation treatment intended to immunocompromised patients or with special diets

    International Nuclear Information System (INIS)

    During and after the treatment of cancer, people with HIV or transplants, the food intake can offer a well-being to the patient, because the action of eating right helps people to feel strong. Healthy people have their immune system working properly and can tolerate small amounts of bacteria. However, immunocompromised persons may not be able to and however immunocompromised people cannot fight this small amount of microorganisms and require a diet with very low microbiological count to avoid contact with potentially harmful bacteria. This is called neutropenic diet. These patients are susceptible to food contamination, so that it's not advisable the ingestion of raw products. The vegetable irradiation with low doses has the purpose of reducing the microbial load. The aim of this study was to obtain data on microbiological, sensorial e physicochemical aspects in minimally processed 'Primavera' salad irradiated with different doses of gamma radiation designed to immunocompromised patients. It were used doses of 1.0kGy, 2.0kGy, 3.0kGy and 4.0kGy irradiated in the Multipurpose Irradiator 60Co located in Radiation Technology Center. Microbiological analyzes were performed (n=25) using Petrifilm, sensory analysis using the sensory acceptance test (n=30) and triangular (n=15) and texture analysis (n=90) in the Food Microbiology Laboratory located at the Radiation Technology Center. The texture analysis was performed with the aid of a texturometer equipped with 5 kg load cell using a triangular cutting Warner-Bratzler Knife blade probe with the descent speed of 2mm/sec. All results were expressed in Newtons (N). The results showed that for microbiological analyzes the standards were followed according to the RDC 12/01 ANVISA. For the sensory test of acceptation no difference was found among the samples, however in the triangular test could be found slight differences between samples irradiated with 4.0kGy and control and there was significant difference

  20. Do termites avoid carcasses? Behavioral responses depend on the nature of the carcasses.

    Directory of Open Access Journals (Sweden)

    Kok-Boon Neoh

    Full Text Available BACKGROUND: Undertaking behavior is a significant adaptation to social life in enclosed nests. Workers are known to remove dead colony members from the nest. Such behavior prevents the spread of pathogens that may be detrimental to a colony. To date, little is known about the ethological aspects of how termites deal with carcasses. METHODOLOGY AND PRINCIPAL FINDINGS: In this study, we tested the responses to carcasses of four species from different subterranean termite taxa: Coptotermes formosanus Shiraki and Reticulitermes speratus (Kolbe (lower termites and Microcerotermes crassus Snyder and Globitermes sulphureus Haviland (higher termites. We also used different types of carcasses (freshly killed, 1-, 3-, and 7-day-old, and oven-killed carcasses and mutilated nestmates to investigate whether the termites exhibited any behavioral responses that were specific to carcasses in certain conditions. Some behavioral responses were performed specifically on certain types of carcasses or mutilated termites. C. formosanus and R. speratus exhibited the following behaviors: (1 the frequency and time spent in antennating, grooming, and carcass removal of freshly killed, 1-day-old, and oven-killed carcasses were high, but these behaviors decreased as the carcasses aged; (2 the termites repeatedly crawled under the aging carcass piles; and (3 only newly dead termites were consumed as a food source. In contrast, M. crassus and G. sulphureus workers performed relatively few behavioral acts. Our results cast a new light on the previous notion that termites are necrophobic in nature. CONCLUSION: We conclude that the behavioral response towards carcasses depends largely on the nature of the carcasses and termite species, and the response is more complex than was previously thought. Such behavioral responses likely are associated with the threat posed to the colony by the carcasses and the feeding habits and nesting ecology of a given species.

  1. Trends in wine microbiology.

    Science.gov (United States)

    Ramón, D

    1997-12-01

    During the last few years many winemakers have started to use pure Saccharomyces cerevisiae strains, frequently isolated from their own geographical regions, to produce wines of more reproductable quality. This microbiological simplification has opened the way for the genetic modification of wine yeast strains. This review concerns the application of molecular techniques in oenology, not only from the point of view of the construction of recombinant strains but also for the study of the population dynamics of wine fermentations.

  2. 9 CFR 310.9 - Anthrax; carcasses not to be eviscerated; disposition of affected carcasses; hides, hoofs, horns...

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Anthrax; carcasses not to be...-MORTEM INSPECTION § 310.9 Anthrax; carcasses not to be eviscerated; disposition of affected carcasses...; general cleanup and disinfection. (a) Carcasses found before evisceration to be affected with...

  3. Heterogeneity of variances for carcass traits by percentage Brahman inheritance.

    Science.gov (United States)

    Crews, D H; Franke, D E

    1998-07-01

    covariances estimated from the model accounting for heterogeneous variances resulted in genetic correlations of or near unity. These results suggest that different genetic values be considered for genetic evaluation of carcass yield and shear force traits from steers with different degrees of Brahman inheritance.

  4. Microbiological evaluation of precooked goat "buchada" Avaliação microbiológica da "buchada" caprina pré-cozida

    Directory of Open Access Journals (Sweden)

    Roberto Germano Costa

    2006-09-01

    Full Text Available The objective of the present work was to evaluate the microbiological characteristics of the precooked goat "buchada", a typical dish in North East of Brazil, composed mainly by goat viscera such as heart, lungs, liver, spleen, intestines, stomach and blood. Samples of "buchada" produced at the different areas of the State of Paraíba, Brazil, presented a high number of aerobic mesophilic bacteria, with values ranging from 5.5 to 6.9 log10 CFU/g. All samples presented total coliforms, and 96.6% presented fecal coliforms, with values between 2.3 and 5.0 log10 NMP/g. The occurrence of Staphylococcus aureus was observed in one sample only, at a proportion of 4.0 log10 CFU/g. Salmonella was absent in all samples. Water activity was around 0.98 and pH close to neutrality except for the samples collected in Campina Grande which presented pH 5.1, significantly lowar (pObjetivou-se com o trabalho avaliar as características microbiológicas da "buchada" caprina pré-cozida, produto típico da culinária nordestina no Brasil, normalmente composta por vísceras, como coração, pulmões, fígado, baço, intestinos, estômago e sangue. As amostras de "buchada" produzidas em diferentes mesorregiões do estado da Paraíba apresentaram elevado número de bactérias mesófilas aeróbias, com valores variando de 5,5 a 6,9 log10 UFC/g. Todas as amostras apresentaram coliformes totais, das quais 96,6% continham coliformes fecais, entre 2,3 e 5,0 log10 NMP/g. A ocorrência de Staphylococcus aureus foi observada apenas em uma amostra proveniente do município de Campina Grande, na proporção de 4,0 log10 UFC/g. Salmonella foi ausente para todas as amostras avaliadas. A atividade de água obtida foi em torno de 0,98, com valores de pH próximos da neutralidade, exceto nas amostras adquiridas em Campina Grande que apresentaram pH em torno de 5,1, diferente (p<0,05 dos demais produtos. Conclui-se com os resultados, condições higiênico-sanitárias inadequadas nas

  5. Expert systems in clinical microbiology.

    Science.gov (United States)

    Winstanley, Trevor; Courvalin, Patrice

    2011-07-01

    This review aims to discuss expert systems in general and how they may be used in medicine as a whole and clinical microbiology in particular (with the aid of interpretive reading). It considers rule-based systems, pattern-based systems, and data mining and introduces neural nets. A variety of noncommercial systems is described, and the central role played by the EUCAST is stressed. The need for expert rules in the environment of reset EUCAST breakpoints is also questioned. Commercial automated systems with on-board expert systems are considered, with emphasis being placed on the "big three": Vitek 2, BD Phoenix, and MicroScan. By necessity and in places, the review becomes a general review of automated system performances for the detection of specific resistance mechanisms rather than focusing solely on expert systems. Published performance evaluations of each system are drawn together and commented on critically.

  6. The case for biocentric microbiology

    Science.gov (United States)

    Aziz, Ramy Karam

    2009-01-01

    Microbiology is a relatively modern scientific discipline intended to objectively study microorganisms, including pathogens and nonpathogens. However, since its birth, this science has been negatively affected by anthropocentric convictions, including rational and irrational beliefs. Among these, for example, is the artificial separation between environmental and medical microbiology that weakens both disciplines. Anthropocentric microbiology also fails to properly answer questions concerning the evolution of microbial pathogenesis. Here, I argue that an exclusively biocentric microbiology is imperative for improving our understanding not only of the microbial world, but also of our own species, our guts, and the world around us. PMID:19653908

  7. Effect of different probiotics on broiler carcass and meat quality

    Directory of Open Access Journals (Sweden)

    ERL Pelicano

    2003-12-01

    Full Text Available The present work evaluated the effect of different probiotics on carcass and meat quality of broilers. One thousand and fifty male Cobb chicks were distributed at one day of age in a randomized design with 3 x 2 + 1 factorial arrangement (3 probiotics, 2 levels of probiotics in drinking water and 1 negative control group, using 5 replications with 30 birds. Carcass yield was higher (p<0.05 in control birds. Nevertheless, the groups fed with probiotics showed higher (p<0.01 leg yield at 45 days of age. There was a significant decrease in color (lightness and increase in pH of breast muscle 5 hours after slaughter in the probiotics treated birds. In the sensory analysis, meat flavor and general aspect 72 hours after slaughter were better when probiotics were added in both water and diet. There were no differences in water holding capacity, cooking loss and shearing force among different probiotics or between them and the control. Thus, meat quality was better when probiotics were fed in the water and diet instead of only in the diet. Nevertheless, carcass and meat quality showed no alteration when the control group was compared to birds fed with probiotics, except for leg yield improvement in the latter.

  8. Rapid microbiology - raising awareness.

    Science.gov (United States)

    Bailie, Jonathan

    2016-01-01

    A 'high-level overview' of some of the emerging rapid microbiology technologies designed to help healthcare engineering and infection control teams working in hospitals and other healthcare facilities more rapidly identify potentially hazardous levels of waterborne microorganisms in their water systems, enabling them to take prompt remedial action, and a look at the some of the 'pros and cons' of such testing techniques, was given by Nalco technical director, Howard Barnes, the vice-chair of the Legionella Control Association (LCA), at a recent LCA open day. HEJ editor, Jonathan Bailie, reports.

  9. National Beef Quality Audit-2011: Harvest-floor assessments of targeted characteristics that affect quality and value of cattle, carcasses, and byproducts

    Science.gov (United States)

    The National Beef Quality Audit-2011(NBQA-2011) was conducted to assess targeted characteristics on the harvest floor that affect the quality and value of cattle, carcasses, and byproducts. Survey teams evaluated approximately 18,000 cattle/carcasses between May and November 2011 in 8 beef processin...

  10. Relationship between carcass traits and phenotypic residual feed intake, breed composition, temperament, and ELISA scores for paratuberculosis in an Angus-Brahman multibreed herd.

    Science.gov (United States)

    Identification of factors that permit animals to grow quickly and efficiently, and have desirable carcass characteristics remains a primary goal in beef production. The objective was to evaluate the relationship between 7 carcass traits and breed group (Angus (A), Brahman (B), Brangus, 3/4 A 1/4 B, ...

  11. Microbial quality, instrumental texture, and color profile evaluation of edible by-products obtained from Barbari goats

    OpenAIRE

    Pramila Umaraw; Pathak, V.; Rajkumar, V; Verma, Arun K.; V. P. Singh; Verma, Akhilesh K.

    2015-01-01

    Aim: The study was conducted to estimate the contribution of edible byproducts of Barbari kids to their live and carcass weight as well as to assess textural and color characteristics and microbiological status of these byproducts. Materials and Methods: Percent live weight, Percent carcass weight, Texture, color, and microbiological analysis was done for edible byproducts viz. liver, heart, kidney, spleen, brain and testicle and longissimus dorsi muscle was taken as a reference. Result...

  12. Microbial quality, instrumental texture, and color profile evaluation of edible by-products obtained from Barbari goats

    OpenAIRE

    Umaraw, Pramila; Pathak, V.; Rajkumar, V; Verma, Arun K.; V. P. Singh; Verma, Akhilesh K.

    2015-01-01

    Aim: The study was conducted to estimate the contribution of edible byproducts of Barbari kids to their live and carcass weight as well as to assess textural and color characteristics and microbiological status of these byproducts. Materials and Methods: Percent live weight, Percent carcass weight, Texture, color, and microbiological analysis was done for edible byproducts viz. liver, heart, kidney, spleen, brain and testicle and longissimus dorsi muscle was taken as a reference. Results: The...

  13. [Evaluation of the status of patients with severe infection, criteria for intensive care unit admittance. Spanish Society for Infectious Diseases and Clinical Microbiology. Spanish Society of Intensive and Critical Medicine and Coronary Units].

    Science.gov (United States)

    Olaechea, Pedro M; Alvarez-Lerma, Francisco; Sánchez, Miguel; Torres, Antonio; Palomar, Mercedes; Fernández, Pedro; Miró, José M; Cisneros, José Miguel; Torres, Manuel

    2009-06-01

    Recent studies have shown that early attention in patients with serious infections is associated with a better outcome. Assistance in intensive care units (ICU) can effectively provide this attention; hence patients should be admitted to the ICU as soon as possible, before clinical deterioration becomes irreversible. The objective of this article is to compile the recommendations for evaluating disease severity in patients with infections and describe the criteria for ICU admission, updating the criteria published 10 years ago. A literature review was carried out, compiling the opinions of experts from the Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC, Spanish Society for Infectious Diseases and Clinical Microbiology) and the Sociedad Española de Medicina Intensiva, Crítica y Unidades Coronarias (SEMICYUC, Spanish Society for Intensive Medicine, Critical Care and Coronary Units) as well as the working groups for infections in critically ill patients (GEIPC-SEIMC and GTEI-SEMICYUC). We describe the specific recommendations for ICU admission related to the most common infections affecting patients, who will potentially benefit from critical care. Assessment of the severity of the patient's condition to enable early intensive care is stressed.

  14. MICROBIOLOGY OF ITCHY EARS

    Directory of Open Access Journals (Sweden)

    Vijin Ravindran Nambiar

    2015-04-01

    Full Text Available Objective: To study microbiology of external auditory canal in patients with itchy ears and to also study susceptibility profiles of pathogenic organisms to aid in appropriate management. Materials & Methods: A total of hundred patients were selected. An external ear canal swab was taken. For recovery of bacteria, the samples were emulsified in a solution of BHI broth to study aerobic and anaerobic bacteria. Fungal microbiology was studied by KOH mount and fungal culture. Culture and sensitivity was done for the pathogenic organisms. Results: Of the total hundred patients, 48% patients had no growth. There were no anaerobes isolated. Of the remaining 52% cases, 33% of the growth was aerobic bacteria and 19% of the growth was fungi. Of the aerobic bacteria, coagulase negative staphylococcus was isolated from 22 patients, staphylococcus aureus from 9 patients and pseudomonas aeruginosa from 2 patients. Of the fungal species, candida was isolated from 11 patients and aspergillus niger from 8 patients. Conclusion: Our study concluded that there need not be an underlying bacterial or fungal infection to cause itching as evidenced by a condition called asteatosis. Hence, asteatosis should be considered as one of the differential diagnosis for chronic and persistent itching when all other causes have been ruled out. We also found that topical ciprofloxacin drops is equally effective against the common bacterial pathogens.

  15. Microbiologically active nanocomposite media

    Science.gov (United States)

    Petranovskii, Vitalii; Panina, Lyudmila; Bogomolova, Eugenia; Belostotskaya, Galina

    2003-07-01

    The most recent approach to the development of novel antimicrobial and antifungal agents is based on the application of synthetic and natural zeolites, because zeolites are known to be the carrier and slow releaser of the heavy metals with olygodynamic properties. The microbiological activity of the ion-exchanged zeolites is attributed to the ionic state of the metal sreleased from the zeolites by ion re-exchange. In the present work we used low cost natural clinoptilolite (Cli) as a substrate for copper and silver in different states. The state of oxidation of the exchanged metal in zeolite with supported Cu and Ag species (in the form of cations, small clusters, sub-coloidal particles, large particles) in order to fit them to fulfill the following criteria: to demonstrate their high protective abilities against fungi and long-term stability. The study of structure of samples with XRD, UV-visible spectroscopy, FTIR, their stability with temperature and during storage was carried out for obtaining the correct correlation with microbiological activity.

  16. Characterization of biological types of cattle (Cycle VIII): carcass, yield, and longissimus palatability traits.

    Science.gov (United States)

    Wheeler, T L; Cundiff, L V; Shackelford, S D; Koohmaraie, M

    2010-09-01

    The objective of this experiment was to evaluate alternative sources of tropically adapted cattle germplasm and compare them with Angus- (AN) and Hereford- (HE) sired steers. Carcass, yield, and longissimus thoracis palatability traits from F(1) steers (n = 621) obtained from mating AN and MARC III cows to HE, AN, Brangus (BR), Beefmaster (BM), Bonsmara (BO), or Romosinuano (RO) sires were compared. Data were adjusted to constant age (426 d), carcass weight (340 kg), fat thickness (1.0 cm), fat trim percentage (25%), and marbling (Small(00)) endpoints. For Warner-Bratzler and slice shear force and trained and untrained sensory panel traits, data were obtained on LM from ribeye steaks stored at 2 degrees C for 14 or 15 d postmortem. The following comparisons were from the age-constant endpoint. Carcasses from BM-, AN-, and BR-sired steers (358, 355, and 351 kg, respectively) were heavier (P < 0.05) than carcasses from steers from HE (343 kg) and BO (331 kg) sires; RO-sired steers (318 kg) had the lightest (P < 0.05) carcasses. Adjusted fat thicknesses for AN- and BM-sired steers (1.3 and 1.2 cm, respectively) were greater (P < 0.05) than for steers from BR (1.0 cm) and BO (0.9 cm) sires; RO-sired steers (0.8 cm) had the least fat thickness. Longissimus areas were larger (P < 0.05) for BO- and BR-sired steers (84.4 and 84.1 cm(2), respectively) than for BM- and HE-sired steers (80.8 and 80.2 cm(2), respectively). A greater (P < 0.05) percentage of carcasses from AN-sired steers graded USDA Choice (69%) than other sire breeds (17 to 47%) except HE (52%). Carcass yield of boneless, totally trimmed retail product was least (P < 0.05) for AN-sired steers (60.1%) and greatest (P < 0.05) for RO- and BO-sired steers (64.4 to 63.5%). Considering all measurements, AN LM tended to be more tender and BM LM tended to be least tender. American composite breeds BM and BR were heavier, fatter, lesser yielding, with similar marbling scores but less tender LM than BO and RO. Angus

  17. The chemical composition of carcasses can be predicted from proxy traits in finishing male beef cattle: A meta-analysis.

    Science.gov (United States)

    Al-Jammas, M; Agabriel, J; Vernet, J; Ortigues-Marty, I

    2016-09-01

    Management practices can contribute to improving carcass quality if carcass quality could be simply evaluated under a wide range of conditions. The objective of this study was to derive quantitative relationships between the most accurate (but laborious) measurements of carcass chemical composition and proxy traits easily obtainable at slaughter (yield grade, subcutaneous fat thickness, marbling, ribeye area and hot carcass weight) by meta-analysis. Data from 34 publications using male beef cattle were used to develop and validate the models. The breeds were characterized according to origin, rate of maturity, production purpose and frame size. The results indicated that the changes in carcass fat and protein can be predicted from the yield grade or subcutaneous fat thickness, and hot carcass weight, with prediction errors ranging between 9 and 12%. Including the breed characteristics in the models did not improve the fit. The relationships are applicable to group values of male beef cattle having light and fatty carcasses from early-maturing British breeds. PMID:27206053

  18. EFSA BIOHAZ Panel (EFSA Panel on Biological Hazards), 2014. Scientific Opinion on the evaluation of the safety and efficacy of peroxyacetic acid solutions for reduction of pathogens on poultry carcasses and meat

    DEFF Research Database (Denmark)

    Hald, Tine; Baggesen, Dorte Lau

    for pathogens (Salmonella and Campylobacter). Spraying appeared to be less effective than dipping in reducing indicator organisms than dipping. When dipping chilled carcasses, reduction of indicator organisms and pathogens was evident, although only in low or medium strength of evidence studies. In chiller bath...... include monitoring of the concentration of HEDP and of the decontaminating substance in the working solution and post-marketing surveillance for resistance in both pathogenic and commensal bacteria....

  19. Recent advances in silage microbiology

    Science.gov (United States)

    Recent advances in our understanding of silage microbiology are reviewed. The ability to extract microbial DNA from silages, amplify portions of DNA, and use the amplified regions to identify strains of microorganisms is at the core of the changes occurring recently in silage microbiology. These dev...

  20. Design of a tablet computer app for facilitation of a molecular blood culture test in clinical microbiology and preliminary usability evaluation

    DEFF Research Database (Denmark)

    Samson, Lasse L.; Pape-Haugaard, Louise; Meltzer, Michelle C.;

    2016-01-01

    BCT app. The study was designed to achieve a high degree of realism as participants carried out a scenario representing the context of use for the MuxBCT app. As the MuxBCT was under development, the scenario involved the use of molecular blood culture tests similar to the MuxBCT for identification...... through specialized applications (apps) while supporting the mobility of the users. The use of apps for mobile phones and tablet computers may support workflow of complex tasks, for example, molecular-based diagnostic tests in clinical microbiology. Multiplex Blood Culture Test (MuxBCT) is a molecular...... and to assist users in reaching an exact bacterial or fungal diagnosis based on blood specimen observations and controls. Additionally, the app allows for entry of test results, and communication thereof to the laboratory information system (LIS). OBJECTIVE: The objective of the study was to describe the design...

  1. EVALUATION OF THE SHELF LIFE AND MICROBIOLOGICAL PARAMETERS IN A CHILLED READY TO EAT MEAT PRODUCT: THE “VITEL TONNÈ”

    Directory of Open Access Journals (Sweden)

    A. Cereser

    2013-02-01

    Full Text Available According to the recent “Hygiene Package” it is the responsibility of Food Business Operator to validate foods’ shelf-life, also considering normal storage and use conditions. The aim of this research was to define the shelf-life of a refrigerated ready-to-eat meat product: the vitel tonnè. 175 samples taken from a producer were stored at 2 different temperatures: +3°C (as declared in label and +8±1°C (in order to simulate the thermal abuses during transport and domestic storage and analyzed at different intervals for microbiological parameters as TVC at 30°C, Lactic Acid Bacteria, Listeria monocytogenes, Salmonella spp., Sulphate-Reducing Clostridia, B. cereus, Coagulase-positive Staphylococci, Enterobacteriaceae and Pseudomonas spp Preliminary results show that temperature abuse shorten shelf-life significantly, mainly for the parameters TVC and LAB.

  2. Carcass characteristics of lambs fed diets containing silage of different genotypes of sorghum

    Directory of Open Access Journals (Sweden)

    Juraci Marcos Alves Suassuna

    2014-02-01

    Full Text Available Thirty-five feedlot lambs (without defined breed, aged between 5 and 7 months, with average live weight of 17.7±3.7 kg were used in a completely randomized design to evaluate the effect of diets containing different genotypes of sorghum on morphometric measurements and qualitative characteristics of carcass and yields of primal cuts. The animals stayed in individual indoor pens for 42 days and slaughtered at an average weight of 26.24 kg. No significant differences were observed on morphometric measurements, hot (11.67 kg and cold (11.39 kg carcass weight, hot (44.46% and cold (43.37% carcass yields, biological yield and on cooling losses. There was also no significant effect of silages of different genotypes of sorghum on the weights and yields of retail cuts (neck, shoulder, rib, loin and leg and on the subjective evaluation of carcasses. It is possible to finish sheep without defined breed feeding them diets based on silages of sorghum, resulting in carcasses with high yield and good conformation.

  3. Microbiology studies in the Space Shuttle

    Science.gov (United States)

    Taylor, G. R.

    1976-01-01

    Past space microbiology studies have evaluated three general areas: microbe detection in extraterrestrial materials; monitoring of autoflora and medically important species on crewmembers, equipment, and cabin air; and in vitro evaluations of isolated terrestrial species carried on manned and unmanned spaceflights. These areas are briefly reviewed to establish a basis for presenting probable experiment subjects applicable to the Space Shuttle era. Most extraterrestrial life detection studies involve visitations to other heavenly bodies. Although this is not applicable to the first series of Shuttle flights, attempts to capture meteors and spores in space could be important. Human pathogen and autoflora monitoring will become more important with increased variety among crewmembers. Inclusion of contaminated animal and plant specimens in the space lab will necessitate inflight evaluation of cross-contamination and infection potentials. The majority of Shuttle microbiology studies will doubtless fall into the third study area. Presence of a space lab will permit a whole range of experimentation under conditions similar to these experienced in earth-based laboratories. The recommendations of various study groups are analyzed, and probable inflight microbiological experiment areas are identified for the Life Sciences Shuttle Laboratory.

  4. Carcass composition of market weight pigs subjected to heat stress in utero and during finishing.

    Science.gov (United States)

    Cruzen, S M; Boddicker, R L; Graves, K L; Johnson, T P; Arkfeld, E K; Baumgard, L H; Ross, J W; Safranski, T J; Lucy, M C; Lonergan, S M

    2015-05-01

    Objectives were to investigate the effects of prolonged gestational and/or postnatal heat stress on performance and carcass composition of market weight pigs. Pregnant gilts were exposed to gestational heat stress (GHS, 28°C to 34°C, diurnal) or thermal neutral (18°C to 22°C, diurnal) conditions during the entire gestation or during the first or second half of gestation. At 14 wk of age (58 ± 5 kg), barrows were housed in heat stress (32°C, HS) or thermal neutral (21°C, TN) conditions. Feed intake and BW were recorded weekly, and body temperature parameters were monitored twice weekly until slaughter (109 ± 5 kg). Organs were removed and weighed, and loin eye area (LEA) and back fat thickness (BF) were measured after carcass chilling. Carcass sides were separated into lean, separable fat, bone, and skin components and were weighed. Moisture, lipid, and protein content were determined in the LM at the 10th rib. Data were analyzed using a split plot with random effect of dam nested within gestational treatment. Carcass measurements included HCW as a covariate to control for weight. Planned orthogonal contrast statements were used to evaluate the overall effect of GHS in the first half, second half, or any part of gestation. Gestational heat stress did not alter postnatal performance or most body temperature parameters (P > 0.10). However, ADFI in the finishing period was increased (P treatments (P = 0.48). The LM from HS carcasses had a greater moisture to protein ratio (P = 0.04). HS barrows also had decreased heart (P < 0.001) and kidney (P < 0.0001) as a percent of BW compared with TN pigs. In summary, GHS may affect head and bone development, subsequently affecting carcass composition. Chronic HS during finishing results in longer times to reach market weight and a leaner carcass once market weight is achieved. PMID:26020353

  5. Characterization of biological types of cattle (Cycle VIII): carcass, yield, and longissimus palatability traits.

    Science.gov (United States)

    Wheeler, T L; Cundiff, L V; Shackelford, S D; Koohmaraie, M

    2010-09-01

    The objective of this experiment was to evaluate alternative sources of tropically adapted cattle germplasm and compare them with Angus- (AN) and Hereford- (HE) sired steers. Carcass, yield, and longissimus thoracis palatability traits from F(1) steers (n = 621) obtained from mating AN and MARC III cows to HE, AN, Brangus (BR), Beefmaster (BM), Bonsmara (BO), or Romosinuano (RO) sires were compared. Data were adjusted to constant age (426 d), carcass weight (340 kg), fat thickness (1.0 cm), fat trim percentage (25%), and marbling (Small(00)) endpoints. For Warner-Bratzler and slice shear force and trained and untrained sensory panel traits, data were obtained on LM from ribeye steaks stored at 2 degrees C for 14 or 15 d postmortem. The following comparisons were from the age-constant endpoint. Carcasses from BM-, AN-, and BR-sired steers (358, 355, and 351 kg, respectively) were heavier (P Carcass yield of boneless, totally trimmed retail product was least (P < 0.05) for AN-sired steers (60.1%) and greatest (P < 0.05) for RO- and BO-sired steers (64.4 to 63.5%). Considering all measurements, AN LM tended to be more tender and BM LM tended to be least tender. American composite breeds BM and BR were heavier, fatter, lesser yielding, with similar marbling scores but less tender LM than BO and RO. Angus carcasses were similar in size, fatter, lesser yielding, with more marbling and more tender LM compared with BM and BR. Bonsmara and RO provide tropically adapted germplasm and produce carcasses that are lighter, leaner, greater yielding, with similar marbling and LM that tend to be more tender than carcasses from BM and BR.

  6. The Effect of Choice Feeding Based on Threonine on Performance and Carcass Parameters of Male Broiler Chicks

    Directory of Open Access Journals (Sweden)

    Tugay Ayasan

    2014-04-01

    Full Text Available The aim of this study was to evaluate the effect of choice feeding based on threonine on performance characteristics and carcass parameters of male broiler chicks. Day old, thirty Ross 308 male chicks were divided into two dietary treatment groups. In the experiment, choice feding did not affect on body weight gain, feed conversion ratio. Feed intake and carcass parameters significantly affected by experimental treatments.

  7. Características da carcaça de caprinos mestiços Anglo-Nubiano, Boer e sem padrão racial definido Evaluation of carcass characteristics of crossbred Anglo-Nubian, Boer and undefined breed goats

    Directory of Open Access Journals (Sweden)

    Antônio Nunes de Oliveira

    2008-08-01

    Full Text Available O objetivo deste trabalho foi estudar as características da carcaça de cabritos de três grupos genéticos: ¾ Anglo Nubiana (n=21, ¾ Boer (n=22 e Sem Padrão Racial Definido (n=21. Os animais foram abatidos em três diferentes pesos: 20, 25 e 30 kg de peso vivo. Avaliaram-se as características de rendimento e conformação da carcaça, bem como as perdas por resfriamento. Os resultados mostraram que não houve interação entre o genótipo e o peso de abate em todas as variáveis estudadas. O genótipo teve efeito significativo (P0,05 entre os grupos genéticos e entre os diferentes pesos de abate. Em conclusão, cabritos mestiços ¾ Boer e ¾ Anglo Nubiano apresentaram características similares de rendimento e conformação de carcaça, sendo ambos superiores aos SRD, e, portanto, mais indicados para produção de carne.The aim of this work was to study the carcass characteristics of three genetic groups: ¾ Anglo-Nubian (n=21, ¾ Boer (n=22 and undefined breed (n=21. The animals were slaughtered at three different weights: 20, 25 and 30kg of live weight. It was evaluated the yield and the conformation carcass characteristics as well as the losses by cooling. The results showed no interaction genetic group and slaughter weight in all studied variables. The genotype had significant effect (P0.05 between genetic groups and weight at slaughter. In conclusion, ¾ Boer and ¾ Anglo-Nubian crossbreed kids had presented similar characteristics of yield and conformation carcass, both superior to the SRD, being therefore more indicated for meat production.

  8. Heterogeneity of variances for carcass traits by percentage Brahman inheritance.

    Science.gov (United States)

    Crews, D H; Franke, D E

    1998-07-01

    correlations of or near unity. These results suggest that different genetic values be considered for genetic evaluation of carcass yield and shear force traits from steers with different degrees of Brahman inheritance.

  9. Transdisciplinarity and Microbiology Education

    Directory of Open Access Journals (Sweden)

    Vittorio Capozzi

    2012-02-01

    Full Text Available A graphical representation of the transdisciplinary pyramid, as a tool, helps us in the design of practical classroom activities that deal with transdisciplinarity, microbiology, and, more generally, with biology. As regard to classroom implementation, we propose two main practical approaches. On the one hand, we can concretize/realize transdisciplinarity through a new design of the lesson, which shall rely on a team of four teachers/professors representing disciplines from all the four levels of the pyramid. On the other hand, a class debate with a teacher–moderator shall be used as a Trojan horse to launch the insights into the newly proposed point of view. The presence of four teachers/professors provides a rigorous study of the topics together with a concretized high-quality transdisciplinarity. Conversely, the class discussion promotes student involvement and the development of critical thought.

  10. Successful Application of Active Learning Techniques to Introductory Microbiology

    Directory of Open Access Journals (Sweden)

    Elizabeth A. Hoffman

    2009-12-01

    Full Text Available While the traditional lecture format may be a successful way to teach microbiology to both medical and nursing students, it was not an effective means of learning for many prenursing and preprofessional students enrolled in either of the introductory microbiology courses at Ashland Community College, an open enrollment institution. The structure of both Medical Microbiology and Principles of Microbiology was redesigned to allow students to address the material in an active manner. Daily quizzes, student group discussions, scrapbooks, lab project presentations and papers, and extra credit projects were all added in order to allow students maximum exposure to the course material in a manner compatible with various methods of learning. Student knowledge, course evaluations, and student success rates have all improved with the active learning format.

  11. EVALUATION OF THE INFLUENCE OF PIG HAM POST – SLAUGHTERING REFRIGERATION ON HYGIENIC PARAMETERS SET IN REGULATION EC 2073/2005

    OpenAIRE

    L. Bassi; S. Bonardi; Brutti, A; C. Bacci; Boni, E; I. Alpigiani; Brindani, F.

    2011-01-01

    In order to evaluate the influence of refrigeration on hygienic parameters, issued by EC Regulation n. 2073:2005 (amended by EC Regulation n. 1441:2007) for swine carcasses, 15 pig hams were tested for microbiological analysis, i.e. enumeration of microorganisms at 30°C and enumeration of enterobacteriacee. Ham swabbing was carried out at the end of slaughtering and after 24 hours of storing in refrigeration cells. The temperature-monitoring recorders were put in the hams at the end of cu...

  12. [Laboratory unification: advantages and disadvantages for clinical microbiology].

    Science.gov (United States)

    Andreu, Antonia; Matas, Lurdes

    2010-10-01

    This article aims to reflect on which areas or tasks of microbiology laboratories could be unified with those of clinical biochemistry, hematology, immunology or pathology laboratories to benefit patients and the health system, as well as the areas that should remain independent since their amalgamation would not only fail to provide a benefit but could even jeopardize the quality of microbiological diagnosis, and consequently patient care. To do this, the distinct analytic phases of diagnosis are analyzed, and the advantages and disadvantages of amalgamation are evaluated in each phase. The pros and cons of the unification of certain areas such as the computer system, occupational risk units, customer service, purchasing logistics, and materials storage, etc, are also discussed. Lastly, the effect of unification on urgent microbiology diagnosis is analyzed. Microbiological diagnosis should be unique. The microbiologist should perform an overall evaluation of the distinct techniques used for a particular patient, both those that involve direct diagnosis (staining, culture, antigen detection techniques or molecular techniques) and indirect diagnosis (antibody detection). Moreover, the microbiology laboratory should be independent, with highly trained technicians and specialists in microbiology that provide added value as experts in infection and as key figures in the process of establishing a correct etiological diagnosis.

  13. Microbiological consequences of indoor composting.

    Science.gov (United States)

    Naegele, A; Reboux, G; Vacheyrou, M; Valot, B; Millon, L; Roussel, S

    2016-08-01

    Recycling of organic waste appeals to more and more people. The aim of this study was to evaluate the microbiological contamination around organic waste bins at three distances over a 12-month period. Contamination near the customary trash of control households was evaluated at the beginning to ensure that there is no recruitment bias. Air samples using the MAS 100 impactor were carried out in 38 dwellings that do household waste composting and in 10 dwellings of controls. Collection of particles by CIP 10 rotating cup sampler and dust samples collected by electrostatic dust collector cloths were acquired in dwellings that do household waste composting. Samples were analyzed by culture and by real-time quantitative PCR. Information about dwelling characteristics and inhabitant practices was obtained by a standardized questionnaire. The genera most often isolated were Penicillium, Aspergillus, Cladosporium and Streptomyces. Near the organic waste bins, bioaerosol samples showed an increase of Acarus siro (P = 0.001). Sedimented dust analyses highlighted an increase of A. siro, Wallemia sebi, Aspergillus versicolor, and Cladosporium sphaerospermum concentrations after a 12-month survey compared to the beginning. Composting favors microorganism development over time, but does not seem to have an effect on the bioaerosol levels and the surface microbiota beyond 0.5 m from the waste bin. PMID:26299932

  14. Effect of Supplementing Spirulina on Live Performance, Carcass Composition and Meat Quality of Japanese Quail

    Directory of Open Access Journals (Sweden)

    Danny Swee Weng CHEONG

    2016-02-01

    Full Text Available This study was conducted to evaluate the effects of different levels of Spirulina (Arthrospira platensis inclusion in feed on live performance, carcass composition and meat quality of Japanese quails during growing stage to identify the best inclusion range for Japanese quails without affecting the growth and carcass parameters. Three hundred Japanese quails of 15 days of age were used in this experiment, randomly divided into 5 groups with 3 replication comprised of 30 males and 30 females. The quails were fed with a basal diet as a control and 4 levels of Spirulina inclusion diet 1, 2, 4 and 8 %. Diets were fed to birds from 15 days to 35 days of age. Body weight gain (BWG, Feed intake (FI, Feed conversion ratio (FCR and Mortality rate (MR were recorded weekly during the experiment. Carcass composition and meat quality tests were done after slaughtering. BWG, FI, FCR and MR were significantly different (p < 0.05 in the experiment. Carcass composition was found to be significantly different in the leg percentage (p < 0.05. Meat color and meat shear force value were also found to differ significantly (p < 0.05 with the Spirulina inclusion diet showing a better result than the control. Therefore, the result of this experiment suggests that diets up to 4 % of Spirulina achieve the best live performance, carcass composition and meat quality.

  15. Acetic acid as an intervention strategy to decontaminate beef carcasses in mexican commercial slaughterhouse

    Directory of Open Access Journals (Sweden)

    Laura Reyes Carranza

    2013-09-01

    Full Text Available Beef can be contaminated during the slaughter process, thus other methods, besides the traditional water washing, must be adopted to preserve meat safety. The objective of this study was to evaluate the effect of 2% acetic acid interventions on the reduction of indicator bacteria on beef carcasses at a commercial slaughterhouse in Mexico. Reduction was measured by the count of mesophilic aerobic bacteria (TPC, total coliform (TC, and fecal coliform (FC (log CFU/ cm². Among the different interventions tested, treatments combining acetic acid solution sprayed following carcass water washing had greater microbial reduction level. Acetic acid solution sprayed at low pressure and longer time (10-30 psi/ 60 s reached higher TPC, TC, and FC reductions than that obtained under high pressure/ shorter time (1,700 psi/ 15 s; P<0.05. Exposure time significantly affected microbial reduction on carcasses. Acetic acid solution sprayed after carcass washing can be successfully used to control sources of indicator bacteria on beef carcasses under commercial conditions.

  16. Carcass traits of Nellore, Caracu. Guzerah and Gir bulls selected for postweaning weight

    Directory of Open Access Journals (Sweden)

    Sarah Figueiredo Martins Bonilha

    2012-01-01

    Full Text Available Data from nine studies were compiled to evaluate the effects of selection for postweaning weight on some carcass characteristics in experimental herds of selected Nellore (NeS, control Nellore (NeC, Caracu (CaS, Guzerah (GuS and Gir (GiS breeds from Instituto de Zootecnia genetic selection program. After the feeding performance test (168 days after weaning, samples of bulls (n=490 from the progeny groups born between 1992 and 2000 were selected for finishing period and slaughtered. Treatments were different across studies. Meta-analysis was conducted with a random coefficients model in which herd was considered a fixed effect and treatments within year and progeny groups as random effects. Slaughter, empty body, hot carcass and cold carcass weights were higher for NeS and CaS, intermediate for GuS and lower for NeC e GiS. CaS bulls had higher proportions of forequarter and spare ribs and lower proportion of hindquarter than the other groups. No significant differences were found for percentage of edible portion among the genetic groups. Selection for postweaning weight in Nellore increased carcass, hindquarter, forequarter, and spare ribs weights without altering dressing percentage, carcass quarter, and hindquarter prime meat cuts proportions.

  17. Meta-analysis in microbiology

    Directory of Open Access Journals (Sweden)

    N Pabalan

    2014-01-01

    Full Text Available The use of meta-analysis in microbiology may facilitate decision-making that impacts public health policy. Directed at clinicians and researchers in microbiology, this review outlines the steps in performing this statistical technique, addresses its biases and describes its value in this discipline. The survey to estimate extent of the use of meta-analyses in microbiology shows the remarkable growth in the use of this research methodology, from a minimal Asian output to a level comparable with those of Europe and North America in the last 7 years.

  18. The food safety value of de-boning finishing pig carcasses with lesions indicative of prior septicaemia

    DEFF Research Database (Denmark)

    Bækbo, A.K.; Petersen, J.V.; Larsen, Marianne Halberg;

    2016-01-01

    procedure concerning carcasses with lesions indicative of a prior septicaemia is not specified. In Denmark, such carcasses are de-boned to avoid the presence of abscesses in the muscles. The aim of this study was to evaluate the food safety value of this specific use of de-boning. Retrospective data from 1...... year, in the form of meat inspection lesion codes for all finishing pigs slaughtered, at the seven largest Danish abattoirs were obtained from the Danish Slaughterhouse Database. These data revealed some differences between abattoirs in the proportion of carcasses sent for de-boning (min: 0.14%; max: 0.......29%; P de-boning (min 0.34%; max 24.14%; P de-boning. Samples from 102 finishing pig carcasses sent for de-boning (due to lesions indicative of prior septicaemia...

  19. Evaluation of the efficacy of a new oral gel as an adjunct to home oral hygiene in the management of chronic periodontitis. A microbiological study using PCR analysis.

    Science.gov (United States)

    Lauritano, D; Bignozzi, C A; Pazzi, D; Palmieri, A; Gaudio, R M; Di Muzio, M; Carinci, F

    2016-01-01

    The use of chemical devices for non-surgical periodontal therapy has led to new treatment strategies aiming primarily at infection control and oral bacterial load. Over the last few decades adjunctive chemical devices has been subjected to many scientific and medical studies. The purpose of the present study was to assess the effect of a new oral gel named Parodongel on the red complex organisms using Polymerase Chain Reaction (PCR) for microbiological analysis. A total of 10 patients with a diagnosis of chronic periodontitis in the age group >25 years, were selected. None of these patients had received any surgical or non-surgical periodontal therapy and demonstrated radiographic evidence of moderate bone loss. Four non-adjacent sites in separate quadrants were selected in each patient for monitoring based on criteria that the sites will localize chronic periodontitis. Microbial analysis (MA) was performed at baseline and at day 15. Paired T-Test was used to detect statistical significant reduction of specific bacteria. The results showed statistically significant reduction of the overall bacterial loading and Treponema Denticola from baseline to day 15. Parodongel can be used as an effective local drug delivery together with oral home care in treatment of chronic periodontitis. PMID:27469558

  20. Periodontal health status in patients treated with the Invisalign® system and fixed orthodontic appliances: A 3 months clinical and microbiological evaluation

    Science.gov (United States)

    Levrini, Luca; Mangano, Alessandro; Montanari, Paola; Margherini, Silvia; Caprioglio, Alberto; Abbate, Gian Marco

    2015-01-01

    Objective: The aim of this prospective study was to compare the periodontal health and the microbiological changes via real-time polymerase chain reaction (PCR) in patients treated with fixed orthodontic appliances and Invisalign® system (Align Technology, Santa Clara, California). Materials and Methods: Seventy-seven patients were enrolled in this study and divided into three groups (Invisalign® group, fixed orthodontic appliances group and control group). Plaque index, probing depth, bleeding on probing were assessed. Total biofilm mass and periodontal pathogens were analyzed and detected via real-time PCR. All these data were analyzed at the T0 (beginning of the treatment) T1 (1-month) and T2 (3 months); and statistically compared using the Mann–Whitney test for independent groups. Results: After 1-month and after 3 months of treatment there was only one sample with periodontopathic anaerobes found in patient treated using fixed orthodontic appliances. The Invisalign® group showed better results in terms of periodontal health and total biofilm mass compared to the fixed orthodontic appliance group. A statistical significant difference (P Invisalign® System show a superior periodontal health in the short-term when compared to patients in treatment with fixed orthodontic appliances. Invisalign® should be considered as a first treatment option in patients with risk of developing periodontal disease. PMID:26430371

  1. Prediction of lean meat proportion of lamb carcasses

    OpenAIRE

    Cadavez, Vasco

    2009-01-01

    The objectives of this study were to identify a reduced pertinent set of variables from an original set of 18 carcass measurements and the development of linear regression models to predict lean meat yield of lamb carcasses. Forty- six male lambs, 26 of Churro Galego Bragançano Portuguese local breed and 20 of Suffolk breed were used. Lambs were slaughtered and carcasses were weighed approximately 30 min after slaughter in order to obtain hot carcass weight (HCW). After cool...

  2. IDENTIFICATIONS OF CARCASS CHARACTERISTIC FOR ESTIMATING THE COMPOSITION OF BEEF CARCASS

    Directory of Open Access Journals (Sweden)

    H. Hafid

    2014-10-01

    Full Text Available The research aimed to identify carcass characteristic that can be used for estimating composition ofbeef carcass. It was used 165 Brahman crossbred cattle in this research. Carcass characteristics wereweight of a half cold carcass (WC ranged from 96 to 151 kg, loin eye area (LEA ranged from 22.09 to304.8 mm2, 12th rib fat thickness (FT12 ranged from 0.80 to 2.90 mm, meat ranged from 53.55 to 90.10kg and carcass fat ranged from 5.54 to 39.72 kg. Result showed that a half weight cold carcass as asingle indicator major may be used to predict meat weight and carcass fat with regression linearequation: Meat (kg = 10.64 + 0.49 WC (R2=0.728 and SE=3.58, and Fat (kg = -21.70 + 0.36 WC**(R2=0.582 and SE=3.65. Multiple regression to predict meat weight based on fat percentage of kidney,pelvic and heart (KPH was Meat (kg = 7.99 + 0.58 WC** - 4.41 KPH** (R2=0.751 and SE=3.43.

  3. Meat goat kids finished on alfalfa, red clover or orchardgrass pastures: carcass merit and meat quality.

    Science.gov (United States)

    Turner, K E; Cassida, K A; Zerby, H N

    2014-12-01

    This experiment was conducted in 2005-2007 to evaluate carcass and chevon (goat meat) quality parameters when meat-goat kids (n=72) were finished on alfalfa (Medicago sativa L; ALF); red clover (Trifolium pratense L.; RCG); or orchardgrass (Dactylis glomerata L.; OGR) pastures. Carcass conformation score was greater (P=0.08) when meat-goat kids were finished on ALF compared to OGR with RCG intermediate. Chevon meat samples from goats finished on the three pasture treatments did not differ in ash, intramuscular fat, or crude protein content or in concentrations of omega6 and omega3 fatty acids, or the omega6 to omega3 ratio. Goats finished on OGR had higher (Pmeat-goat kids finished on ALF, RCG, or ORG produced desirable carcass weights for most niche markets in the USA. Chevon is a low-fat meat option with high desirable fatty acids for human diets. PMID:25089787

  4. Carcass and cut yields and meat qualitative traits of broilers fed diets containing probiotics and prebiotics

    Directory of Open Access Journals (Sweden)

    ERL Pelicano

    2005-09-01

    Full Text Available This study evaluated the use of different probiotics, prebiotics and symbiotics on the quality of carcasses and meat of broiler chickens. One hundred and eight day-old Cobb male broilers were used (n=108 in a completely randomized design according to a 3x3 factorial, with 3 probiotics in the diet (no probiotics, probiotics 1, probiotics 2 and 3 prebiotics in the diet (no prebiotics, prebiotics 1, prebiotics 2. There were nine treatments with 4 replicates and 3 birds per replicate. The results showed that the carcass and cut yields, color (L* - lightness, a* - redness, and b* - yellowness, pH, cooking losses, shearing force and sensory analysis were not affected by the use of different growth promoters at 42 days of age. It was concluded that growth promoters supplemented to the diet did not affect the studied quantitative and qualitative parameters of the carcass and breast meat of broiler chickens.

  5. RESEARCHES ON THE PHENOTYPIC CORRELATION BETWEEN DIFFERENT MEASUREMENTS ON THE CARCASS OF RABBITS

    Directory of Open Access Journals (Sweden)

    DANIELA MARCELA TOBĂ (GOINA

    2013-12-01

    Full Text Available The aim of this experiment was to get more experimental data on newmethodologies for rabbit meat production evaluation. The trial was carried out on30 rabbits. The rabbits were weaned at 35 days, having an average live weight of499.11+25.98 g. Rabbits were all slaughtered on the same day, that was on the 84th day of living (at this time the average live weight was 1701.7+34.07, andsome linear measurements (body length, loin width, chest width, carcass lengthchest dept and hind leg length were performed on cold carcasses. The rabbitshave been kept in wire fattening cages: 6 rabbits / cage.

  6. The crossbreeding of improved Jezersko-Solcˇava sheep with Charollais to improve carcass traits

    OpenAIRE

    Dragomir Kompan; Silvester Zgur; Angela Cividini

    2010-01-01

    Thirteen improved Jezersko-Solcˇava lambs (JSR) and 16 crossbreds with Charollais (JSRxCH) were used to evaluate the effect of genotype and sex on carcass traits. Lambs were weaned at around 60 days of age. They were fed with commercial concentrate and hay ad libitum and slaughtered at 30 kg of average live weight at 103 days of age. Crossbred lambs had at the same live weight at slaughter better conformation (9.19 on 15 points scale) than JSR lambs (7.58). Carcasses of crossbred lambs had lo...

  7. Sensitivity of partial carcass dissection for assessment of porcine cysticercosis at necropsy.

    Science.gov (United States)

    Lightowlers, M W; Assana, E; Jayashi, C M; Gauci, C G; Donadeu, M

    2015-11-01

    Many interventions against Taenia solium are evaluated by assessing changes in the prevalence of porcine cysticercosis ascertained by carcass dissection. Financial and logistical difficulties often prohibit dissection of entire pig carcasses. We assessed 209 pigs from rural areas of Cameroon and Peru for the presence of T. solium cysticerci and determined the distribution of parasites within the musculature of infected animals. Considering the presence of cysts in the tongue, masticatory muscles and heart, 31 of the 38 (81%) naturally infected animals were identified as having cysts. Dissection of only the tongue, masticatory muscles and heart provides a relatively sensitive and highly specific method for diagnosis of porcine cysticercosis. PMID:26385439

  8. Effects of weight at slaughter and sex on the carcass characteristics of Florida suckling kids.

    Science.gov (United States)

    Peña, Francisco; Perea, J; García, A; Acero, R

    2007-03-01

    The effect of slaughter weight and sex on some carcass traits of suckling kids of the Florida breed was evaluated. A total of 60 kids (30 male and 30 female), fed exclusively on milk replacers, were slaughtered at 7-8kg (group 1), 10-11kg (group 2) or 14-15kg (group 3) of liveweight (mean weights of 7.6kg, 10.8kg and 14.4kg, respectively). Higher slaughter weights decreased the percentage of subproducts (blood, skin, head, feet) and internal organs (lungs+traquea, heart, liver, spleen, thymus) but significantly increased the percentage of intestine and fat depots (omental fat and mesenteric fat). Higher slaughter weights also increased carcass measures (L 40.5 vs 49.1; F 22.5 vs 25.9; G 10.4 vs 14.2; Wr 10.1 vs 13.9; Wth 8.0 vs 10.5; Th 16.5 vs 199; B 32.3 vs 42.4; PT 41.5 vs 50.8), compactness carcass index (96.6 vs 152.3) and compactness leg index (27.5 vs 44.1). Sex only significantly affected the percentages of feet, internal organs, omental fat, measure L, carcass compactness index and hind limb compactness index. The meat colour and fat colour were mainly scored as pale and white respectively in the carcasses of the lightest animals, whereas heavier kids were scored as pink and cream. Slaughter weight also influenced significantly the carcass fatness (score 1 in lightest kids and 2 or 3 in heavier ones). There were no significant (p>0.05) differences between slaughter weight group and sex in dressing percentages. Percentages corresponding to the long leg, back and neck (30-33%, 18-19% and 8-10%, respectively) decreased when the slaughter weight increased, whereas the ribs (23-25%) and the flank (10-11%) increased slightly. The carcasses comprised 57-58% muscle, 22-25% bone, 5-6% subcutaneous fat and 9-12% intermuscular fat. The percentage muscle stayed the same with increasing slaughter weight, whereas the bone decreased and the fat increased. The carcasses of the heavier females contained less lean and more fat than the males. The bone percentage was

  9. 9 CFR 314.8 - Dead animal carcasses.

    Science.gov (United States)

    2010-01-01

    ... received with livestock for slaughter at an official establishment, no dead animal or part of the carcass... circumstances shall the carcasses of any animal which has died otherwise than by slaughter, or any part thereof... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Dead animal carcasses. 314.8...

  10. Microbiological Pollution of Water in the Bay of Durrës

    OpenAIRE

    ORNELA SHOSHI; SULEJMAN SULÇE; PJERIN SHOSHI; ZAMIRA RADA

    2014-01-01

    Several microorganisms with human or animal origin can be the cause of the diseases transmitted through water (microbiological effects). The discharges of used waters, contaminated with pathogen microbes, constitute the main cause of the microbiological pollution of the water. The objective of this study is the evaluation of the water microbiological status in Durrës bay, compared with the EEA resolution (European Environmental Agency), with a view of identifying the risk factors and the...

  11. [Microbiological diagnosis of HIV infection].

    Science.gov (United States)

    López-Bernaldo de Quirós, Juan Carlos; Delgado, Rafael; García, Federico; Eiros, José M; Ortiz de Lejarazu, Raúl

    2007-12-01

    Currently, there are around 150,000 HIV-infected patients in Spain. This number, together with the fact that this disease is now a chronic condition since the introduction of antiretroviral therapy, has generated an increasing demand on the clinical microbiology laboratories in our hospitals. This increase has occurred not only in the diagnosis and treatment of opportunistic diseases, but also in tests related to the diagnosis and therapeutic management of HIV infection. To meet this demand, the Sociedad de Enfermedades Infecciosas y Microbiología Clinica (Spanish Society of Infectious Diseases and Clinical Microbiology) has updated its standard Procedure for the microbiological diagnosis of HIV infection. The main advances related to serological diagnosis, plasma viral load, and detection of resistance to antiretroviral drugs are reviewed in this version of the Procedure.

  12. Contextual Analysis of CT Scanned Pig Carcasses

    DEFF Research Database (Denmark)

    Lyckegaard, Allan; Larsen, Rasmus; Christensen, Lars Bager;

    2006-01-01

    knowledge about the weight of other tissue types from CT. Knowing the weight of individual tissue types will directly give access to other measures such as the weight of the carcass and the Lean Meat Percentage (LMP). Until now, most analyses of CT scans have been based on the Hounsfield spectra that does...... not consider the spatial context in CT scan. Applying contextual methods from the field of image analysis we hope to make a virtual dissection of pig carcasses.......Knowledge of the weight of tissue types in pig carcasses is generally only available after manual dissection. The use of computed tomography (CT) has demonstrated to be a promising approach to gain knowledge on the lean meat weight (Romvari, 2005), but less effort has been put into gaining...

  13. Evaluation of the ionizing radiation effects in microbiology, physical and chemical and sensory aspects of ice cream; Avaliacao dos efeitos da radiacao ionizante nos aspectos microbiologicos, fisico-quimicos e sensoriais de sorvetes

    Energy Technology Data Exchange (ETDEWEB)

    Rogovschi, Vladimir Dias

    2015-06-01

    The ice cream is defined as an emulsion of fats and proteins or a mixture of water and sugar, other ingredients may be added provided since they do not affect the product. It is considered a food of high nutritional value, providing lipids, carbohydrates, protein, calcium, phosphorus, and other minerals and vitamins (A, B1, B2, B6, C, D, E and K), and it is considered one of the most important products and higher interest to the dairy industry due to great demand by the consumers. The diseases related to food consumption are considered one of the most significant problems. Several outbreaks related to microbiological contamination of ice cream have been reported in recent decades in Asia, Europe and America. It is believed that the ice cream, as a frozen food, presents no risk to the population health. However, it is considered an excellent environment for the growth of microorganisms due to its composition, pH close to neutrality and long storage period. The aim of this study was to evaluate the microbiological, sensory and physicochemical aspects of ice cream. The ice cream samples were irradiated with gamma rays (60Co) with the doses of LOkGy, 2.0kGy, 3.0kGy and 4.0kGy. The samples intended for the inoculation of Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 11229) and Salmonella abaetetuba (ATCC 35640) have been irradiated with doses of 1.0, 2.0 and 3.0kGy. It can be concluded that the dose of 3.0kGy was adequate to reduce most of the studied microorganisms to undetected levels. The use of gamma radiation affected the texture and the parameters of the colorimetric analyses of the ice cream. The results of the sensorial analyses showed that the better accepted dose was 3.0kGy. (author)

  14. Ratio of meat preparates to carcass in cattle slaughtered in Istanbul

    Directory of Open Access Journals (Sweden)

    Koçak Ömür

    2007-01-01

    Full Text Available This study was performed to determine the relationship between the ratio of the bones and valuable meat preparates to the carcass, the age and the sex parameters in Holstein and Swiss Braun race cattle which are widely breaded in our country. The half and quarter carcasses of the cattle that are slaughtered in Istanbul were used as working material. The carcasses were separated into 4 groups as above 3 years of age group (n=24, under 3 years of age group (n=46, male group (n=53 and female group (n=17. Totally 140 carcasses were evaluated. According to the obtained results, hind quarter weight, fillet, loin, rump, tranche, sirloin, round, flank, shank, brisket, fore loin, sticking, chuck and total bones parameters were significantly different at (p<0.001 level between above the 3 years of age and under the 3 years of age group. Between the same groups the sirloin tip parameter was significantly different at p<0.01 level. At the parameters of leg weight, shank and half carcasses there was no significant difference between the groups. We could not determine any significant differences in the percentage ratio of all meat parameters to the carcass between the groups of above 3 years of age and under 3 years of age. In the male and female groups, all the parameters except loin, leg weight and shank were significantly different between the 2 groups. Hind quarter and shank parameters were significantly different at p<0.05 level, round parameter was significantly different at p<0.01 level, and the other valuable meat propagates were significantly different at p<0.001 level. Rump parameter was evaluated as significantly different at p<0.05 level between male and female groups. The other parameters were not significantly different between the mentioned groups.

  15. MICROBIOLOGICAL TESTING OF SELECTED CONFECTIONERY PRODUCTS QUALITY

    Directory of Open Access Journals (Sweden)

    Ľubomíra Juhaniaková

    2014-02-01

    Full Text Available The aim of this work was to determine microbiological quality and water activity of confectionery products as corpus of desserts, stuffing cakes and finished cakes. In confectionery products microbiological parameters: total count of bacteria, coliforms bacteria, mesophilic aerobic bacteria, yeasts, microscopic filamentous fungi, counts of Staphylococcus aureus and Salmonella spp. were observed. These confectionery products were evaluated: 5 corpus of Kremeš, 5 stuffing of Kremeš and 5 Venček cake. For microbiological tests 15 samples of confectionery products were used. The numbers of total count of bacteria ranged from 2.9 to 3.65 log CFU.g-1, the number of mesophilic aerobic bacteria ranged from 2.00 to 3.28 log CFU.g-1, coliforms bacteria in confectionery products ranged from 0.00 to 3.15 CFU.g-1, number of yeasts ranged from 0.00 to 3.30 log CFU.g-1and the number of microscopic fungi ranged from 0.00 to 2.90 CFU.g-1. None of the samples showed any growth of coliforms bacteria, Staphylococcus aureus and Salmonella spp. From microscopic fungi were isolated genera Alternaria, Aspergillus and Penicillium. Eight from fifteen investigated samples of confectionary products were in accordance with the Codex Alimentarius of the Slovak Republic. The lowest water activity was found in Kremeš corpus samples (0.953 and higher in Venček samples (0.973.

  16. Correlations of visual scores, carcass traits, feed efficiency and retail product yield in Nellore cattle

    Directory of Open Access Journals (Sweden)

    Paulo Henrique Cancian

    2014-02-01

    Full Text Available The growing use of visual scores (VS and ultrasound (US for carcass evaluation in breeding programs, calls for a knowledge of the relationships between these traits and other relevant characteristics, such as feed efficiency and production of commercial cuts. The objective of this study was to evaluate correlations between body visual scores and carcass traits identified by ultrasound (US and feed efficiency (FE, carcass weight (HCW, dressing percentage (DP and retail product yield (RPY in beef cattle. Nellore cattle (male, 42 non-castrated [NCAST] and 44 castrated [CAST] were evaluated by both VS and US, at the postweaning (15-month old and finishing phases (21-month old. Visual scores of conformation (C, precocity (P and muscling (M were assessed and the backfat thickness (UBFT, rump fat thickness (URFT and ribeye area (UREA were measured by ultrasound. Gain-to-feed (G:F ratio and residual feed intake (RFI were measured in feedlot. Hot carcass weight, DP and RPY were determined at harvest. Non-castrated cattle had greater HCW and RPY but lower UBFT and URFT than CAST. Postweaning VS and US were poorly correlated with FE in both sexual conditions. Finishing VS were negatively correlated with G:F in CAST and finishing URFT was negatively correlated with RPY in NCAST. The relationship of VS and US with feed efficiency and meat yield is affected by age at the date of evaluation and by castration. Feed efficiency is not related to the yield of meat cuts in Nellore cattle

  17. Meat goat kids finished on alfalfa, red clover, or orchardgrass pastures: Carcass merit and meat quality

    Science.gov (United States)

    This experiment was conducted in 2005-2007 to evaluate carcass and meat quality parameters when meat goat kids were finished on alfalfa (Medicago sativa L; ALF); red clover (Trifolium pretense L.; RCG); or orchardgrass (Dactylis glomerata L; OGR) pastures. Final shrunk body weights were similar whe...

  18. Predictive Food Microbiology

    DEFF Research Database (Denmark)

    Østergaard, Nina Bjerre

    Listeria monocytogenes is a well-known food borne pathogen that potentially causes listeriosis. No outbreaks or cases of listeriosis have been associated with cottage cheese, but several confirmed cases and outbreaks in the EU and the US have been related to dairy products made from raw or pasteu......Listeria monocytogenes is a well-known food borne pathogen that potentially causes listeriosis. No outbreaks or cases of listeriosis have been associated with cottage cheese, but several confirmed cases and outbreaks in the EU and the US have been related to dairy products made from raw...... or pasteurised milk. This, in combination with the fact that cottage cheese support growth of Listeria monocytogenes, induces a documentation requirement on the food producer. In the EU regulatory framework, mathematical models are recognised as a suitable supplement to traditional microbiological methods...... distributions) were advocated. It is believed that it is necessary to define some applicable methodologies for the development of growth models for complex products such as fermented dairy products. Model development is a comprehensive process with an almost infinite data requirement and the findings...

  19. The microbiology of metalworking fluids.

    Science.gov (United States)

    Saha, Ratul; Donofrio, Robert S

    2012-06-01

    Metalworking fluids (MWFs) are complex mixtures of chemicals and are indispensable materials in industry. They are used as cooling and lubricating agents in different machining process such as grinding, milling, and cutting. The quality of MWFs is affected by physical, chemical, and microbial contaminates. In particular, MWFs are highly vulnerable to microbial contamination, which may act both as potential pathogens and deteriorgens. Microbial contamination is of major concern due to potential health hazards such as skin dermatitis and hypersensitivity pneumonitis. The contaminated MWFs can exhibit high degrees of microbial loading, ranging from 10(4) to 10(10) colony-forming units (CFU)/ml. Wide varieties of microorganisms are reported to colonize MWFs. Traditional culturing techniques are not only laborious and time consuming but also underestimate the actual distribution of the microorganisms present in the contaminated MWFs. Therefore, rapid molecular methods such as real-time PCR and fluorescent in situ hybridization are implemented to monitor the microbial load. In industry, biocides are presently used to control microbial contamination. However, it has its own disadvantages and therefore, in recent years, alternative methods such as UV irradiation were evaluated to reduce microbial contamination in MWFs. Microbes inhabiting the MWF are also capable of forming biofilm which is detrimental to the MWF system. Biofilm is resistant to common disinfectant methods, and thus further research and development is required to effectively control its formation within MWF systems. This review is intended to discuss the overall microbiological aspects of MWF. PMID:22543351

  20. INFLUENCE OF BREED ON CARCASS CUTS COMPOSITION

    Directory of Open Access Journals (Sweden)

    Silvester Žgur

    2000-06-01

    Full Text Available Carcass from 260 Simmental and 159 Brown bulls were dissected first to different cuts (chuck, shoulder, front shank, rib roast, back, loin, tenderloin, brisket, rib, flank, leg and hind shank and then to lean, fat, tendon and bone. The comparison between two breeds was made at 12.5 % carcass fat. Simmental breed had statistically significantly higher share of leg and brisket with rib and lower share of chuck, front and hind shank, rib roast, tenderloin and flank, with higher lean and lower bone percentage, but the differences were relatively small compared to Brown breed.

  1. INFLUENCE OF BREED ON CARCASS CUTS COMPOSITION

    OpenAIRE

    Silvester Žgur; Marko Čepon

    2000-01-01

    Carcass from 260 Simmental and 159 Brown bulls were dissected first to different cuts (chuck, shoulder, front shank, rib roast, back, loin, tenderloin, brisket, rib, flank, leg and hind shank) and then to lean, fat, tendon and bone. The comparison between two breeds was made at 12.5 % carcass fat. Simmental breed had statistically significantly higher share of leg and brisket with rib and lower share of chuck, front and hind shank, rib roast, tenderloin and flank, with higher lean and lower b...

  2. Romanian Quality Pork Carcasses in 2009

    OpenAIRE

    Ioana Mihaela Balan; Cornelia Petroman; Ioan Petroman; Diana Marin; Ramona Ciolac; Loredana Heber; Dora Manuela Orboi

    2010-01-01

    Romanian husbandry is better and better every year. In this context, the most representative results are in pig production, where the quality of carcasses is comparative to that of the other E.U. member states with high performances in this field. The methods of classification we have used were acknowledged by the Commission of Classification of Swine, Bovine, and Sheep Carcasses in Romania, i.e. optic probe (the apparatuses Fat-o-meat’er and OptiGrade-Pro), as well as the ZP (Zwei Punkte) me...

  3. Microbiological and physicochemical characterization of milk according to the seasons

    Directory of Open Access Journals (Sweden)

    Rafael Fagnani

    2014-06-01

    Full Text Available The seasons have a major influence on milk production, especially when animals are exposed to climatic variations. This situation is very common in Brazil and further investigation is needed to understand the losses caused by oscillations in milk constituents. The aim was to evaluate how seasonality can affect the physicochemical and microbiological quality of raw milk in Ivaiporã region, state of Paraná, Brazil. In this research, the milk from properties in northern Paraná was evaluated during 24 months for lactose, protein, fat, total solids, total bacteria count and somatic cell count. The regional rainfall and average temperature recorded at each station were analyzed for determination of Thermal Comfort Index and evaluation of heat stress. This study concluded that seasonality have influence on microbiological and physicochemical quality of milk in Ivaiporã region, with higher percentages of solids in the fall and inferior microbiological quality in the spring.

  4. [Authorized Qualifications of Staff Conducting Examinations in the Field of Clinical Microbiology].

    Science.gov (United States)

    Nishiyama, Hiroyuki

    2015-04-01

    Because of the increase in healthcare-associated infections, appearance of highly resistant bacteria, and that of emerging/re-emerging infectious diseases, it is necessary for the skills of clinical microbiological technologists and the associated technology to be improved. Technologist in Microbiology (4,717 certified) and Specialist in Microbiology (58 certified) are authorized qualifications in the field of examination for clinical microbiology, with a history of 60 years, and Clinical Microbiological Technologist (670 certified) and Infection Control Microbiological Technologist (ICMT) (528 certified) are necessary qualifications to become a member of an infection control team. As problems to be resolved, clarifying the relationships among the authorized qualifications, reconsidering the fairness of evaluating written examinations, and further consideration of the administration method for an increasing number of examinees need to be tackled.

  5. Influences of Stocking Salmon Carcass Analogs on Salmonids in Yakima River Tributaries, 2001-2002 Technical Report.

    Energy Technology Data Exchange (ETDEWEB)

    Pearsons, Todd N.; Johnson, Christopher L. (Washington Department of Fish and Wildlife, Olympia, WA)

    2003-04-01

    The benefits that marine derived nutrients from adult salmon carcasses provide to juvenile salmonids are increasingly being recognized. Current estimates suggest that only 6-7% of marine-derived nitrogen and phosphorus that were historically available to salmonids in the Pacific Northwest are currently available. Food limitation may be a major constraint limiting the restoration of salmonids. A variety of methods have been proposed to offset this nutrient deficit including: allowing greater salmon spawning escapement, stocking hatchery salmon carcasses, and stocking inorganic nutrients. Unfortunately, each of these methods has some ecological or socio-economic shortcoming. We intend to overcome many of these shortcomings by making and evaluating a pathogen free product that simulates a salmon carcass (analog). Abundant sources of marine derived nutrients are available such as fish offal from commercial fishing and salmon carcasses from hatcheries. However, a method for recycling these nutrients into a pathogen free analog that degrades at a similar rate as a natural salmon carcass has never been developed. We endeavored to (1) develop a salmon carcass analog that will increase the food available to salmonids, (2) determine the pathways that salmonids use to acquire food from analogs, and (3) determine the benefits to salmonids and the potential for application to salmonid restoration. We used a before-after-control-impact-paired design in six tributaries of the upper Yakima basin to determine the utility of stocking carcass analogs. Our preliminary results suggest that the introduction of carcass analogs into food-limited streams can be used to restore food pathways previously provided by anadromous salmon. The analogs probably reproduced both of the major food pathways that salmon carcasses produce: direct consumption and food chain enhancement. Trout and salmon fed directly on the carcass analogs during the late summer and presumably benefited from the increased

  6. Partial ablation of uropygial gland effect on carcass characteristics of Akar Putra chicken.

    Science.gov (United States)

    Jawad, Hasan S A; Idris, L H; Bakar, Z B; Kassim, A B

    2016-08-01

    This study evaluated the effect of partial uropygialectomy (PU) on carcass traits of male and female Akar Putra chickens. Sixty chicks of each sex were evenly distributed into 5 treatment groups with 3 replicates per group containing 4 males and 4 females each, and reared for 12 wk. Homogeneity of the groups was satisfied with regard to the parity. Experimental treatments consisted of a control treatment (T1), and partial ablation of the uropygial gland was applied on the second, third, fourth, and fifth treatments at 3, 4, 5, and 6 wk of age, respectively. The chickens were fed ad libitum the same diets (1 to 13 d: starter; 14 d to slaughter: finisher). On the last d of the experiment, 12 birds were randomly selected from each treatment group (2 males and 2 females per replicate) and slaughtered to determine carcass characteristics, which included carcass weight, dressing percentage with or without eating giblets, and the relative weights of heart, liver, gizzard, thighs, wings, breast, back, and neck. From the results of the study, it was shown that the partial ablation of the uropygial gland at all ages had certain dependent effects concerning some carcass parameters, as shown by higher breast and back relative weights in males and breast relative weight in females. As a consequence, a positive effect also was noticed regarding the carcass morphology in terms of the increase in dressing percentage with or without eating giblets thus leading to an increase in the body weight and carcass weight. Furthermore, the best result was obtained in the second treatment when PU was applied at 3 wk of age compared with other experimental groups. Moreover, the current study provides a novel and economic alternative to enhance the body performance of poultry in general and Akar Putra chicken particularly. PMID:27081194

  7. Microbiological Methodology in Astrobiology

    Science.gov (United States)

    Abyzov, S. S.; Gerasimenko, L. M.; Hoover, R. B.; Mitskevich, I. N.; Mulyukin, A. L.; Poglazova, M. N.; Rozanov, A. Y.

    2005-01-01

    Searching for life in astromaterials to be delivered from the future missions to extraterrestrial bodies is undoubtedly related to studies of the properties and signatures of living microbial cells and microfossils on Earth. As model terrestrial analogs of Martian polar subsurface layers are often regarded the Antarctic glacier and Earth permafrost habitats where alive microbial cells preserved viability for millennia years due to entering the anabiotic state. For the future findings of viable microorganisms in samples from extraterrestrial objects, it is important to use a combined methodology that includes classical microbiological methods, plating onto nutrient media, direct epifluorescence and electron microscopy examinations, detection of the elemental composition of cells, radiolabeling techniques, PCR and FISH methods. Of great importance is to ensure authenticity of microorganisms (if any in studied samples) and to standardize the protocols used to minimize a risk of external contamination. Although the convincing evidence of extraterrestrial microbial life will may come from the discovery of living cells in astromaterials, biomorphs and microfossils must also be regarded as a target in search of life evidence bearing in mind a scenario that alive microorganisms had not be preserved and underwent mineralization. Under the laboratory conditions, processes that accompanied fossilization of cyanobacteria were reconstructed, and artificially produced cyanobacterial stromatolites resembles by their morphological properties those found in natural Earth habitats. Regarding the vital importance of distinguishing between biogenic and abiogenic signatures and between living and fossil microorganisms in analyzed samples, it is worthwhile to use some previously developed approaches based on electron microscopy examinations and analysis of elemental composition of biomorphs in situ and comparison with the analogous data obtained for laboratory microbial cultures and

  8. Antibiotic expected effectiveness and cost under real life microbiology: evaluation of ertapenem and ceftriaxone in the treatment of community-acquired pneumonia for elderly patients in Spain

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    Grau S

    2014-02-01

    Full Text Available Santiago Grau,1 Virginia Lozano,2 Amparo Valladares,3 Rafael Cavanillas,4 Yang Xie,5 Gonzalo Nocea3 1Hospital del Mar, Barcelona, Spain; 2Pharmacoeconomics and Outcomes Research Iberia, Madrid, Spain; 3Outcomes Research Merck Sharp and Dohme Ltd, Madrid, Spain; 4Medical Affairs, Merck Sharp and Dohme Ltd, Madrid, Spain; 5Global Health Outcomes, Merck Sharp and Dohme Ltd, Whitehouse Station, NJ, USA Background: Clinical efficacy of antibiotics may be affected by changes in the susceptibility of microorganisms to antimicrobial agents. The purpose of this study is to assess how these changes could affect the initial efficacy of ertapenem and ceftriaxone in the treatment of community-acquired pneumonia (CAP in elderly patients and the potential consequences this may have in health care costs. Methods: Initial efficacy in elderly was obtained from a combined analysis of two multicenter, randomized studies. An alternative scenario was carried out using initial efficacy data according to the pneumonia severity index (PSI. Country-specific pathogens distribution was obtained from a national epidemiological study, and microbiological susceptibilities to first- and second-line therapies were obtained from Spanish or European surveillance studies. A decision analytic model was used to compare ertapenem versus ceftriaxone for CAP inpatient treatment. Inputs of the model were the expected effectiveness previously estimated and resource use considering a Spanish national health system perspective. Outcomes include difference in proportion of successfully treated patients and difference in total costs between ertapenem and ceftriaxone. The model performed one-way and probabilistic sensitivity analyses. Results: First-line treatment of CAP with ertapenem led to a higher proportion of successfully treated patients compared with ceftriaxone in Spain. One-way sensitivity analysis showed that length of stay was the key parameter of the model. Probabilistic

  9. Establishing molecular microbiology facilities in developing countries.

    Science.gov (United States)

    Ahmed, Salman S; Alp, Emine; Ulu-Kilic, Aysegul; Doganay, Mehmet

    2015-01-01

    Microbiology laboratories play an important role in epidemiology and infection control programs. Within microbiology laboratories, molecular microbiology techniques have revolutionized the identification and surveillance of infectious diseases. The combination of excellent sensitivity, specificity, low contamination levels and speed has made molecular techniques appealing methods for the diagnosis of many infectious diseases. In a well-equipped microbiology laboratory, the facility designated for molecular techniques remains indiscrete. However, in most developing countries, poor infrastructure and laboratory mismanagement have precipitated hazardous consequences. The establishment of a molecular microbiology facility within a microbiology laboratory remains fragmented. A high-quality laboratory should include both conventional microbiology methods and molecular microbiology techniques for exceptional performance. Furthermore, it should include appropriate laboratory administration, a well-designed facility, laboratory procedure standardization, a waste management system, a code of practice, equipment installation and laboratory personnel training. This manuscript lays out fundamental issues that need to be addressed when establishing a molecular microbiology facility in developing countries.

  10. Romanian Quality Pork Carcasses in 2009

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    Ioana Mihaela Balan

    2010-10-01

    Full Text Available Romanian husbandry is better and better every year. In this context, the most representative results are in pig production, where the quality of carcasses is comparative to that of the other E.U. member states with high performances in this field. The methods of classification we have used were acknowledged by the Commission of Classification of Swine, Bovine, and Sheep Carcasses in Romania, i.e. optic probe (the apparatuses Fat-o-meat’er and OptiGrade-Pro, as well as the ZP (Zwei Punkte method. Most Romanian pork carcasses (over 99.0% range among E and U high-quality classes. This is more than satisfactory from the point of view of the qualitative evolution of pork in Romania. The results obtained by the Romanian pig breeders were remarkable in 2009, i.e. superior compared to those of the previous years. The implementation of the EUROP system for pork carcass classification in Romania has largely generated expected results.

  11. Genetic parameters for carcass cut weight in Irish beef cattle.

    Science.gov (United States)

    Pabiou, T; Fikse, W F; Näsholm, A; Cromie, A R; Drennan, M J; Keane, M G; Berry, D P

    2009-12-01

    The objective of this study was to estimate genetic parameters for the weights of different wholesale cuts, using an experimental and a commercial data set. The experimental and commercial data sets included 413 and 635 crossbred Belgian Blue, Charolais, Limousin, Angus, Holstein, and Simmental animals, respectively. Univariate analyses using a mixed linear animal model with relationships were undertaken to estimate the heritability of cold carcass weight, carcass conformation and fat, and the cut weights, whereas a series of bivariate analyses was used to estimate the phenotypic and genetic correlations between carcass weight, carcass conformation, carcass fat, and the major primal cuts. Heritability estimates for cold carcass weight in both data sets were moderate (>0.48), whereas heritability estimates for carcass conformation and fat grading were greater in the commercial data set (>0.63) than in the experimental study (>0.33). Across both data sets, heritability estimates for wholesale cut weight in the forequarter varied from 0.03 to 0.79, whereas heritability estimates of carcass cut weight in the hindquarter varied from 0.14 to 0.86. Heritability estimates for cut weights expressed as a proportion of the entire carcass weight varied from 0.04 to 0.91. Genetic correlations were strong among the different carcass cut weights within the experimental and the commercial studies. Genetic correlations between the weights of selected carcass cuts and carcass weight were moderate to high (minimum 0.45; maximum 0.88) in both data sets. Positive genetic correlations were observed in the commercial data set between the different wholesale cut weights and carcass conformation, whereas these were positive and negative in the experimental data set. Selection for increased carcass weight will, on average, increase the weight of each cut. However, the genetic correlations were less than unity, suggesting a benefit of more direct selection on high value cuts. PMID:19717761

  12. Feed efficiency indexes and their relationships with carcass, non-carcass and meat quality traits in Nellore steers.

    Science.gov (United States)

    Nascimento, M L; Souza, A R D L; Chaves, A S; Cesar, A S M; Tullio, R R; Medeiros, S R; Mourão, G B; Rosa, A N; Feijó, G L D; Alencar, M M; Lanna, D P D

    2016-06-01

    Five hundred and seventy-five Nellore steers were evaluated for residual feed intake and residual feed intake and gain and their relationships between carcass, non-carcass and meat quality traits. RFI was measured by the difference between observed and predicted dry matter intake and RIG was obtained by the sum of -1*RFI and residual gain. Efficient and inefficient animals were classified adopting ±0.5 standard deviations from RFI and RIG mean. A mixed model was used including RFI or RIG and contemporary group as fixed effects, initial age as covariate and sire and experimental period as random effects, testing the significance of the regression slope for each evaluated trait. RIG was positively related to longissimus muscle area. Efficient-RFI animals had lower liver and internal fat proportions compared to inefficient-RFI animals. Efficient-RFI and efficient-RIG animals had 11.8% and 11.2% lower extracted intramuscular fat, compared to inefficient-RFI and inefficient-RIG animals, respectively. Efficient-RFI animals had tougher meat compared to inefficient-RFI animals. PMID:26874085

  13. [The modern microbiology in the clinical managing].

    Science.gov (United States)

    Casal Román, Manuel

    2012-01-01

    The tuberculosis is one of the most important and mortal diseases of the world. The microbiological confirmatory diagnosis and the microbiological therapeutic orientation are fundamental nowadays in the tuberculosis in AIDS and in the Resistant tuberculosis. They are described throughout the time by the classic Microbiology: From 1882 to final 20th century (130 years). With the modern current Microbiology: In the beginning of the 21st century (20-30 years). And as will be done with the future Microbiology: From the years 2020-30. The important advances are outlined in the modern and future clinical microbiology, for the control of the Tuberculosis.

  14. Microbiological analysis of water used in hydrotherapy

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    M. F. Perestrelo

    2006-01-01

    Full Text Available Water used in hydrotherapy units of Nova Iguaçu and Nilópolis, Rio de Janeiro State, Brazil, was microbiologically analyzed. Thirty samples (5ml each were weekly collected from September 2001 to June 2002 before the beginning and after the end of activities in the units. For analysis, routine techniques were used, which showed the presence of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella spp, Candida spp, Penicillium spp, Epidermophyton spp, Aspergillus fumigatus, Aspergillus niger, Aspergillus spp, Cephalosporium spp, Cladosporium spp, Trichophyton rubrum, and Trichophyton spp. Results indicated a need for improving hygienic conditions, suggesting that water might be a contamination source in the evaluated units.

  15. Learning Medical Microbiology and Infectious Diseases by Means of a Board Game: Can It Work?

    Science.gov (United States)

    Struwig, Magdalena C.; Beylefeld, Adriana A.; Joubert, Georgina

    2014-01-01

    Innovative teaching and learning is increasingly becoming part of medical education. We report the evaluation of a medical microbiology board game, Med Micro Fun With Facts (MMFWF), based on Trivial Pursuit™ principles. The game was developed to stimulate medical students' interest in microbiology and expose students to the subject content of…

  16. Impact of sorting before feeding zilpaterol hydrochloride on feedlot performance and carcass characteristics of yearling steers.

    Science.gov (United States)

    Hilscher, F H; Hussey, E M; Nuttelman, B L; Burken, D B; Griffin, W A; Vander Pol, K J; Hutcheson, J P; Erickson, G E

    2015-05-01

    Two studies evaluated sorting and feeding zilpaterol hydrochloride (ZH) on feedlot performance and carcass characteristics in randomized block-designed finishing trials. In Exp. 1 (initial BW 342 ± 10 kg, = 1,000), 5 treatments included an unsorted non-ZH fed negative control (-CON), an unsorted ZH fed positive control (+CON), and 3 treatments in which the heaviest 20% within the pen were sorted and marketed 28 d early and the remaining 80% were fed ZH. The 20% were identified at the beginning (EARLY), 100 d from slaughter (MIDDLE), or 50 d from slaughter (LATE). Because of sorting, the remaining steers in sorted treatments were fed 14 d longer than -CON and +CON. Average days on feed for control treatments were 165 and 173 d for the EARLY, MIDDLE, and LATE treatments. In Exp. 2 (initial BW 376 ± 29 kg, = 1,400), 4 treatments included -CON; +CON; an early weight sort fed ZH (1-SORT) with the heaviest 20% identified at d 1 and sorted 50 d from harvest and marketed 14 d before -CON and +CON, with the remaining 80% of the pen fed 7 d longer than -CON and +CON; and a 4-way sort 50 d from harvest fed ZH (4-SORT) with steers sorted into HEAVY, MID-HEAVY, MID-LIGHT, and LIGHT groups marketed -14, 0, +7, and +28 d from -CON and +CON, respectively. Average days on feed for control treatments were 154 and 157 d for the 1-SORT and 159 d for 4-SORT. Steers were fed Zilmax at 8.3 mg/kg DM for 20 d followed by a 3 d withdrawal. In Exp. 1, steers fed +CON had 13 kg greater (P treatments. Percentage of overweight carcasses (454 kg) was greater (P ≤ 0.05) in sorted treatments than in -CON. In Exp. 2, HCW for +CON was 15 kg heavier (P 0.10) between +CON and -CON, whereas carcass weight SD of 4-SORT was reduced (P < 0.01) compared with that of -CON and +CON. Steers fed ZH had a greater percentage of carcasses over 454 kg than steers fed -CON (P < 0.01). Although not statistically different (P = 0.27), the percentage of carcasses over 454 kg was reduced by 28% for 4-SORT compared

  17. Use of a commercial household steam cleaning system to decontaminate beef and hog carcasses processed by four small or very small meat processing plants in Georgia.

    Science.gov (United States)

    Trivedi, Suvang; Reynolds, A Estes; Chen, Jinru

    2007-03-01

    Small and very small meat-processing facilities in the United States are in need of a pathogen reduction technology that would be both effective and economical. In the present study, the effectiveness of a commercial household steam cleaner for reducing naturally occurring bacterial populations on freshly slaughtered beef and hog carcasses was evaluated in four small or very small meat-processing plants. Three anatomical sites on the right half of each carcass were exposed to a 60-s steam treatment, and the corresponding left half of the carcass remained untreated. Samples were collected from 72 beef and 72 hog carcasses before, immediately after, and 24 h after the steam treatment. The mean populations of total aerobes, coliforms, and Enterobacteriaceae recovered from three anatomical sites on the beef carcasses were 1.88, 1.89, and 1.36 log CFU/cm2, respectively, before the steam treatment, 1.00, 0.71, and 0.52 log CFU/cm2, respectively, immediately after the steam treatment, and 1.10, 0.95, and 0.50 log CFU/cm2, respectively, 24 h after the steam treatment. On hog carcasses, the mean populations of total aerobes, coliforms, and Enterobacteriaceae recovered from the three anatomical sites were 2.50, 2.41, and 1.88 log CFU/cm2, respectively, before the steam treatment, 0.50, 0.94, and 0.21 log CFU/cm2, respectively, immediately after the steam treatment, and 0.91, 1.56, and 0.44 log CFU/cm2, respectively, 24 h after the steam treatment. The steam treatment significantly reduced the total aerobes, coliforms, and Enterobacteriaceae at all three anatomical locations on both types of carcasses (P steam treatment was midline > neck > rump for beef carcasses and belly > jowl > ham for hog carcasses except for the total coliform counts at the midline and neck areas on the beef carcasses. Of the 144 carcasses evaluated, 5 (3.47%) were positive for Salmonella before steam treatment, but all carcasses tested negative for Salmonella after the treatment. Results indicate that

  18. Production of Ceratonova shasta Myxospores from Salmon Carcasses: Carcass Removal Is Not a Viable Management Option.

    Science.gov (United States)

    Foott, J S; Stone, R; Fogerty, R; True, K; Bolick, A; Bartholomew, J L; Hallett, S L; Buckles, G R; Alexander, J D

    2016-06-01

    Severe infection by the endemic myxozoan parasite, Ceratonova (synonym, Ceratomyxa) shasta, has been associated with declines in and impaired recovery efforts of populations of fall-run Chinook Salmon Oncorhynchus tshawytscha in the Klamath River, California. The parasite has a complex life cycle involving a polychaete worm host as well as a salmon host. Myxospore transmission of this parasite, from salmon to polychaete, is a life cycle step during which there is a potential for applied disease management. A 3-year data set on prevalence, intensity, and spore characteristics of C. shasta myxospores was obtained from adult Chinook Salmon carcasses surveyed in the main stem of the Klamath River and three of its tributaries, Bogus Creek and the Shasta and Trinity rivers. Annual prevalence of myxospore detection in salmon intestines ranged from 22% to 52%, and spore concentration values per intestinal scraping ranged from 3.94 × 10(2) to 1.47 × 10(7) spores. A prevalence of 7.3% of all carcasses examined produced >5.0 × 10(5) spores, and these carcasses with "high" spore counts accounted for 76-95% of the total spores in a given spawning season. Molecular analysis of visually negative carcasses showed that 45-87% of these samples had parasite DNA, indicating they contained either low spore numbers or presporogonic stages of the parasite. Myxospores were rarely found in carcasses of freshly spawned adults but were common in decomposed carcasses of both sexes. The date of collection or age (based indirectly on FL) did not influence detection. The longer prespawn residence time for spring-run Chinook Salmon compared with that for fall-run Chinook Salmon in the Trinity River was associated with higher spore loads. The dye exclusion method for assessing spore viability in fresh smears indicated an inverse relationship in spore integrity and initial spore concentration. A carcass-removal pilot project in Bogus Creek for 6 weeks in the fall of 2008 (907 carcasses removed

  19. Production of Ceratonova shasta Myxospores from Salmon Carcasses: Carcass Removal Is Not a Viable Management Option.

    Science.gov (United States)

    Foott, J S; Stone, R; Fogerty, R; True, K; Bolick, A; Bartholomew, J L; Hallett, S L; Buckles, G R; Alexander, J D

    2016-06-01

    Severe infection by the endemic myxozoan parasite, Ceratonova (synonym, Ceratomyxa) shasta, has been associated with declines in and impaired recovery efforts of populations of fall-run Chinook Salmon Oncorhynchus tshawytscha in the Klamath River, California. The parasite has a complex life cycle involving a polychaete worm host as well as a salmon host. Myxospore transmission of this parasite, from salmon to polychaete, is a life cycle step during which there is a potential for applied disease management. A 3-year data set on prevalence, intensity, and spore characteristics of C. shasta myxospores was obtained from adult Chinook Salmon carcasses surveyed in the main stem of the Klamath River and three of its tributaries, Bogus Creek and the Shasta and Trinity rivers. Annual prevalence of myxospore detection in salmon intestines ranged from 22% to 52%, and spore concentration values per intestinal scraping ranged from 3.94 × 10(2) to 1.47 × 10(7) spores. A prevalence of 7.3% of all carcasses examined produced >5.0 × 10(5) spores, and these carcasses with "high" spore counts accounted for 76-95% of the total spores in a given spawning season. Molecular analysis of visually negative carcasses showed that 45-87% of these samples had parasite DNA, indicating they contained either low spore numbers or presporogonic stages of the parasite. Myxospores were rarely found in carcasses of freshly spawned adults but were common in decomposed carcasses of both sexes. The date of collection or age (based indirectly on FL) did not influence detection. The longer prespawn residence time for spring-run Chinook Salmon compared with that for fall-run Chinook Salmon in the Trinity River was associated with higher spore loads. The dye exclusion method for assessing spore viability in fresh smears indicated an inverse relationship in spore integrity and initial spore concentration. A carcass-removal pilot project in Bogus Creek for 6 weeks in the fall of 2008 (907 carcasses removed

  20. Effects of Duroc Breeding Lines on Carcass Composition and Meat Quality.

    Science.gov (United States)

    Choi, Jung-Seok; Jin, Sang-Keun; Choi, Yang-Il; Lee, Jae-Joon

    2015-01-01

    This study was performed to investigate the carcass composition and pork quality of Duroc breeding lines in Korea. A total of 200 Duroc pigs were used, and those were originated from four different great-grandparent (GGP) breeding stock farms (L1: N farm, L2: W farm, L3: S farm, L4: R farm). The carcasses of pigs from these farms were collected, and meat quality traits were evaluated. L1 and L2 had smaller carcass weights and thin backfat, whereas L3 and L4 had heavy carcass weights and thick backfat. L3 and L4 had higher contents of fat and protein than L1 and L2. For the meat quality characteristics, L1, L2, and L4 had higher pH values than L3. In addition, L4 had higher water holding capacity than the other lines. L4 had the highest sensory evaluation scores with regard to both juiciness and flavor. Consequently, the study results indicate that pork quality information from domestic Duroc breeding stock lines could be used to effectively improve pork quality in Korea. PMID:26761803

  1. [Post-mortem microbiology analysis].

    Science.gov (United States)

    Fernández-Rodríguez, Amparo; Alberola, Juan; Cohen, Marta Cecilia

    2013-12-01

    Post-mortem microbiology is useful in both clinical and forensic autopsies, and allows a suspected infection to be confirmed. Indeed, it is routinely applied to donor studies in the clinical setting, as well as in sudden and unexpected death in the forensic field. Implementation of specific sampling techniques in autopsy can minimize the possibility of contamination, making interpretation of the results easier. Specific interpretation criteria for post-mortem cultures, the use of molecular diagnosis, and its fusion with molecular biology and histopathology have led to post-mortem microbiology playing a major role in autopsy. Multidisciplinary work involving microbiologists, pathologists, and forensic physicians will help to improve the achievements of post-mortem microbiology, prevent infectious diseases, and contribute to a healthier population.

  2. Prevalence of Campylobacter Jejuni and Coli in Sheep Carcasses by Using

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    Reza Shahrokhabadi

    2013-11-01

    Full Text Available Background: Campylobacter species are common bacterial pathogens causing gastroenteritis in humans worldwide. Materials and Methods: A total of 148 randomly sheep carcasses were sampled by surface section of neck meat taken immediately after slaughter analyzed using microbiological examinations. Results: Campylobacter spp. was isolated from 10.13% meat cultures samples examined. Among these 80% sample were C. jejuni and 20% sample were C. coli. Using PCR assays, the number of positive campylobacters increased to 11.48%. Of these positive samples, 82.35% were C. jejuni and 17.65% were C. coli. Significantly higher prevalence rates of Campylobacter spp. (p<0.05 were found in the meat samples taken in summer (47.05%. Conclusion: The PCR is a reliable and sensitive method which can be used as a diagnostic technique for the detection of campylobacter in lamb samples.

  3. Evaluation of sunflower meal on growth and carcass traits of finishing pigs Avaliação de farelo de girassol no desempenho e características de carcaca de suínos em terminação

    Directory of Open Access Journals (Sweden)

    Douglas de Carvalho Carellos

    2005-02-01

    Full Text Available With the objective to evaluate the influence of increasing levels of inclusion of sunflower meal (SFM solvent extraction (DM: 92.71%, CP: 27.50%, NDF: 43.57%, ADF: 32.96%, CF: 25.91%, EE: 3,08% and GE: 4390 kcal on the performance and carcass characteristics of finishing swine, a performance trial was conducted in the Department of Animal Science of the Federal University of Lavras - MG. A total of 80 crossbred pigs (LD x LW with initial weight of 62.20 ± 4.21 kg were utilized, one barrow and one gilt per experimental unit, allocated in a randomized block design. The treatments were represented by five diets with increasing levels of inclusion (0, 4, 8, 12 and 16% of SFM. At the end of the experimental period, all pigs were slaughtered (99.80 ± 6.91kg and submitted to the carcass evaluation by the Brazilian Method of Carcass Evaluation (ABCS, 1973. The increasing levels of SFM shown a linear reduction (P0.05 for any increasing levels of SFM in the diets, however, sex effect was found, in the barrows, which shown higher ETM, P2, AG and RCG and lower AOL in relation to the females In conclusion Sunflower meal should be included up to 16% in pig diet (isocaloric and isolysinic, basis with no major effects on growth performance or carcass traits of finishing pigs.Com o objetivo de avaliar a influência de níveis crescentes de inclusão do farelo de girassol (FG em rações (MS: 92,71%; PB: 27,50%; FDN: 43,57%; FDA: 32,96%; FB: 25,91% e a atuação deste no desempenho e características de carcaça para suínos em terminação, conduziu-se uma pesquisa no Departamento de Zootecnia da Universidade Federal de Lavras-MG. Foram utilizados 80 suínos mestiços (LD x LW com peso inicial de 62,20 ± 4,21 kg, sendo 1 macho e 1 fêmea por baia, distribuídos em um delineamento em blocos ao acaso. As rações apresentaram níveis crescentes de inclusão (0, 4, 8, 12 e 16% de FG. Ao final do ensaio de desempenho os 80 suínos foram abatidos (99,80 ± 6,91kg

  4. Transforming a Sequence of Microbiology Courses Using Student Profile Data

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    Rosa J. Buxeda

    2009-12-01

    Full Text Available A study was performed in the General Microbiology and Industrial Microbiology courses to increase research awareness at an early stage of the educational process and to establish collaboration between students in an Industrial Microbiology program and industry. In both courses, the professor helped students determine their learning styles and then used these data to design activities in order to accomplish the above objectives. In both the treatment and the control sections, students learned about strategies to optimize learning based on their learning styles. A cooperative learning format was introduced to promote active learning and team-building skills. The diverse learning styles data profile was used by students during cooperative learning activities for effective team integration. In the General Microbiology course, a mentor-mentee structure was introduced to expose students to research in microbiology by visiting research facilities on campus. This structure was an addition to the regular curriculum, which meets American Society for Microbiology curriculum recommendations. The results suggest an increase in interest in research by students. In the Industrial Microbiology course, a strategy was introduced to establish collaboration with industry in which students visit the workplace and identify microbial processes, microbiologist roles, and skills needed by microbiologists. Evaluation of these topics using pre- and posttest data indicates a significant increase in acquired knowledge relevant to daily workplace environments with the reformed course. In both courses, students gain information early in their academic experience to help them consider participation in research experiences while providing them with real-world experience toward the end of their academic careers, when they see the need for it.

  5. Quality control for diagnostic oral microbiology laboratories in European countries

    Directory of Open Access Journals (Sweden)

    Andrew J. Smith

    2011-11-01

    Full Text Available Participation in diagnostic microbiology internal and external quality control (QC processes is good laboratory practice and an essential component of a quality management system. However, no QC scheme for diagnostic oral microbiology existed until 2009 when the Clinical Oral Microbiology (COMB Network was created. At the European Oral Microbiology Workshop in 2008, 12 laboratories processing clinical oral microbiological samples were identified. All these were recruited to participate into the study and six laboratories from six European countries completed both the online survey and the first QC round. Three additional laboratories participated in the second round. Based on the survey, European oral microbiology laboratories process a significant (mean per laboratory 4,135 number of diagnostic samples from the oral cavity annually. A majority of the laboratories did not participate in any internal or external QC programme and nearly half of the laboratories did not have standard operating procedures for the tests they performed. In both QC rounds, there was a large variation in the results, interpretation and reporting of antibiotic susceptibility testing among the laboratories. In conclusion, the results of this study demonstrate the need for harmonisation of laboratory processing methods and interpretation of results for oral microbiology specimens. The QC rounds highlighted the value of external QC in evaluating the efficacy and safety of processes, materials and methods used in the laboratory. The use of standardised methods is also a prerequisite for multi-centre epidemiological studies that can provide important information on emerging microbes and trends in anti-microbial susceptibility for empirical prescribing in oro-facial infections.

  6. Carcass Type Affects Local Scavenger Guilds More than Habitat Connectivity.

    Science.gov (United States)

    Olson, Zachary H; Beasley, James C; Rhodes, Olin E

    2016-01-01

    Scavengers and decomposers provide an important ecosystem service by removing carrion from the environment. Scavenging and decomposition are known to be temperature-dependent, but less is known about other factors that might affect carrion removal. We conducted an experiment in which we manipulated combinations of patch connectivity and carcass type, and measured responses by local scavenger guilds along with aspects of carcass depletion. We conducted twelve, 1-month trials in which five raccoon (Procyon lotor), Virginia opossum (Didelphis virginiana), and domestic rabbit (Oryctolagus spp.) carcasses (180 trials total) were monitored using remote cameras in 21 forest patches in north-central Indiana, USA. Of 143 trials with complete data, we identified fifteen species of vertebrate scavengers divided evenly among mammalian (N = 8) and avian species (N = 7). Fourteen carcasses (9.8%) were completely consumed by invertebrates, vertebrates exhibited scavenging behavior at 125 carcasses (87.4%), and four carcasses (2.8%) remained unexploited. Among vertebrates, mammals scavenged 106 carcasses, birds scavenged 88 carcasses, and mammals and birds scavenged 69 carcasses. Contrary to our expectations, carcass type affected the assemblage of local scavenger guilds more than patch connectivity. However, neither carcass type nor connectivity explained variation in temporal measures of carcass removal. Interestingly, increasing richness of local vertebrate scavenger guilds contributed moderately to rates of carrion removal (≈6% per species increase in richness). We conclude that scavenger-specific differences in carrion utilization exist among carcass types and that reliable delivery of carrion removal as an ecosystem service may depend on robust vertebrate and invertebrate communities acting synergistically. PMID:26886299

  7. Evaluation of the Effect of Probiotic (Inersan®) Alone, Combination of Probiotic with Doxycycline and Doxycycline Alone on Aggressive Periodontitis – A Clinical and Microbiological Study

    OpenAIRE

    Shah, Mishal Piyush; Gujjari, Sheela Kumar; Chandrasekhar, Veerendrakumar Siddhpur

    2013-01-01

    Introduction: The purpose of the present study was to evaluate the effect of a probiotic (Inersan®) alone, a combination of the probiotic with doxycycline and doxycycline alone on aggressive periodontitis patients.

  8. Avaliação das condições microbiológicas em unidades de alimentação e nutrição Evaluation of microbiological conditions in food service stablishiments

    Directory of Open Access Journals (Sweden)

    Nélio José de Andrade

    2003-06-01

    se encontram, os restaurantes avaliados devem determinar, numa fase inicial, metas a serem atingidas. Em etapas posteriores, deverão atender a recomendações mais exigentes.The microbiological quality of the air in 63 processing areas, in the hands of 68 manipulator personnel, and on 36 equipments and utensils in food service establishments were evaluated. No more than 19% and 32% of the air in processing areas were in good hygienic conditions for mesophilic aerobics, and molds and yeasts, respectively. The number of microorganisms in those areas was below the APHA recommendation, 30 cfu/cm2/per week. As there is no microbiological specification or recommendation for manipulators, in this study, five ranges of microbiological numbers per hand were established (105, in cfu per hand. For mesophilic aerobic bacteria, it was determined the following percentages in the different ranges 12, 21, 35, 19, and 13%, for total coliforms: 54, 27, 13, 6, and 0%, for molds and yeasts: 63, 21, 13, 14, and 0%, and for S. aureus 72, 13, 16, 0, and 0%. Some results showed an inadequate personal hygiene procedure. For mesophilic aerobics, the results showed that 19% of the equipment and utensil surfaces were in good hygienic conditions, according to APHA recommendations (2 cfu/cm2. Using the same recommendations for total coliforms and molds and yeasts, it could be observed that 46 and 45% of the surfaces, respectively, were in good hygienic conditions. It was observed that is necessary to establish standards or specifications more adequate to control microbiological conditions in Brazilian food service establishments. Firstly, these establishments should determine goals for microbiological specification to be reached. After that, they should attend harder specifications.

  9. Phenotypic and genetic relationships of feed efficiency with growth performance, ultrasound, and carcass merit traits in Angus and Charolais steers.

    Science.gov (United States)

    Mao, F; Chen, L; Vinsky, M; Okine, E; Wang, Z; Basarab, J; Crews, D H; Li, C

    2013-05-01

    Feed efficiency is of particular importance to the beef industry, as feed costs represent the single largest variable cost in beef production systems. Selection for more efficient cattle will lead to reduction of feed related costs, but should not have adverse impacts on quality of the carcass. In this study, we evaluated phenotypic and genetic correlations of residual feed intake (RFI), RFI adjusted for end-of-test ultrasound backfat thickness (RFIf), and RFI adjusted for ultrasound backfat thickness and LM area (RFIfr) with growth, ultrasound, and carcass merit traits in an Angus population of 551 steers and in a Charolais population of 417 steers. In the Angus steer population, the phenotypic and genetic correlation of RFI with carcass merit traits including HCW, carcass backfat, carcass LM area, lean meat yield, and carcass marbling were not significant or weak with correlations coefficients ranging from -0.0007 ± 0.05 to 0.18 ± 0.21. In the Charolais steer population, the phenotypic and genetic correlations of RFI with the carcass merit traits were also weak, with correlation coefficients ranging from -0.07 ± 0.06 to 0.19 ± 0.18, except for the genetic correlation with carcass average backfat, which was moderate with a magnitude of 0.42 ± 0.29. Inclusion of ultrasound backfat thickness in the model to predict the expected daily DMI for maintenance explained on average an additional 0.5% variation of DMI in the Angus steers and 2.3% variation of DMI in the Charolais steer population. Inclusion of both the ultrasound backfat and LM area in the model explained only 0.7% additional variance in DMI in the Angus steer population and only 0.6% in the Charolais steer population on top of the RFIf model. We concluded that RFIf adjusted for ultrasound backfat at the end of the test will lead to decreases of both the phenotypic and genetic correlations with carcass backfat and marbling score to a greater extent for late-maturing beef breeds such as Charolais than

  10. 42 CFR 493.821 - Condition: Microbiology.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Condition: Microbiology. 493.821 Section 493.821 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES... These Tests § 493.821 Condition: Microbiology. The specialty of microbiology includes, for purposes...

  11. 21 CFR 866.2540 - Microbiological incubator.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Microbiological incubator. 866.2540 Section 866.2540 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Microbiology Devices § 866.2540...

  12. 42 CFR 493.909 - Microbiology.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Microbiology. 493.909 Section 493.909 Public Health... Proficiency Testing Programs by Specialty and Subspecialty § 493.909 Microbiology. The subspecialties under the specialty of microbiology for which a program may offer proficiency testing are...

  13. Performance and carcass characteristics of young cattle fed with soybean meal treated with tannins.

    Science.gov (United States)

    Mezzomo, Rafael; Paulino, Pedro Veiga Rodrigues; Barbosa, Marcília Medrado; da Silva Martins, Taiane; Paulino, Mario Fonseca; Alves, Kaliandra Souza; Gomes, Daiany Iris; Dos Santos Monnerat, João Paulo Ismério

    2016-06-01

    This study aimed to evaluate the effects of replacement of soybean meal (SBM) with soybean meal treated with tannin (SBMT) on the intake, digestibility, performance and characteristics of the carcasses of young cattle fed a high-concentrate diet. Forty-two Nellore bulls with body weight of 244.5 ± 4.99 kg were used. Diets had the inclusion of 7.5% SBM, with a proportion of that SBM (0, 33, 66 or 100%) replaced for SBMT; and other treatment (SBMT + urea) just with 2.5% of SBM which was treated with tannins. Seven animals were randomly selected and slaughtered, and the remaining animals were distributed on treatments and remained for 112 days. After, all animals were slaughtered. There was a linear decline in dry matter intake (P = 0.026) when SBM was replaced with SBMT. No decrease in carcass weight (P > 0.05) was observed. The efficiency of carcass weight gain showed a quadratic function effect (P = 0.049). There were changes in carcass gain composition when SBMT was added (P < 0.05), with an increase in muscle and reduction in fat deposition. The use of SBMT in place of SBM causes changes in body gain composition in animals and reduces DM intake by the animals, achieving a better feed conversion efficiency. PMID:26355192

  14. Performance, carcass and meat quality of ewes supplemented with magnesium oxide

    Directory of Open Access Journals (Sweden)

    Camila Constantino

    2014-01-01

    Full Text Available The objective of this study was to evaluate the performance, the carcass and meat characteristics of ewes supplemented with magnesium oxide. Eighteen 6-year-old Santa Ines ewes were used in a completely randomized experimental design, where three levels of supplementation (0.0, 0.1 and 0.2% magnesium oxide in the concentrate were tested, with six replicates. Final weight, weight gain, feed intake and feed conversion were not affected by levels of magnesium supplementation. Hot and cold carcass weights showed linear increasing and quadratic responses, respectively, according to supplementation with magnesium. Carcass measurements such as length, depth, and measures of arm and leg were not affected by supplementation. Depth and width of the longissimus muscle and rib-eye area were not affected; however, marbling and ether extract increased linearly with supplementation. Water loss and color were not affected. Lipid oxidation and shear force were not affected by supplementation, while the myofibrillar fragmentation index showed a quadratic regression. There was a linear decrease in pH with magnesium supplementation. Supplementation with magnesium can improve carcass and meat pH, but can act negatively by increasing the amount of marbling and ether extract of meat.

  15. Residual feed intake and its effect on carcass and meat characteristics of feedlot Zebu cattle

    Directory of Open Access Journals (Sweden)

    Julimar do Sacramento Ribeiro

    2012-06-01

    Full Text Available The objective of this study was to evaluate the residual feed intake (RFI and its relation with performance, carcass and meat characteristics of Zebu animals. A total of 40 Zebu steers with initial age of 18 months and initial live weight of 350 kg were confined in feedlot for 112 days. The average daily gain (ADG and dry matter intake (DMI were obtained every 14 days. Residual feed intake was determined by the difference between observed and predicted DMI, based on the metabolic weight and the ADG. Animals were classified into the RFI groups: high (>0.5 standard deviation of the mean - least efficient, medium (±0.5 standard deviation of the mean and low (<0.5 standard deviation of the mean - most efficient. Daily cost with feeding and per kilogram of weight gain was determined. Characteristics of carcass (weight, yield, subcutaneous fat meat (pH, shear force and color were assessed. Animals with low RFI presented lower DMI. The variation in DMI between the low and the high RFI groups promoted difference in the daily cost with feeding and per kilogram of weight gain, with animals of low RFI presenting costs 5 and 9% lower, respectively. Carcass and meat characteristics differed over the RFI groups. The knowledge of the RFI of the animals enables a more efficient system, with reduction in the costs with feeding, without promoting alterations on the performance and carcass characteristics of the meat from Zebu animals.

  16. Feedlot performance, feed efficiency and carcass traits in Nellore cattle selected for postweaning weight

    Directory of Open Access Journals (Sweden)

    Tatiana Lucila Sobrinho

    2013-02-01

    Full Text Available The objective of this study was to evaluate the correlated responses to selection for growth in feed efficiency and carcass traits of Nellore animals. A total of 121 animals of the Nellore control (NeC and selection herds were subjected to performance testing and classified regarding residual feed intake (RFI. Sixty-seven of these animals were selected and finished until reaching 4 mm of subcutaneous fat thickness between the 12th and 13th ribs. The animals were slaughtered at a mean age of 541 days and mean live weight of 423 kg for NeS and of 363 kg for NeC, and carcass traits were measured. Animals in NeS presented higher initial and slaughter weights, dry matter intake, hot and cold carcass weight, weight of retail cuts, and carcass yield than NeC animals for the same slaughter age and feedlot time. No significant differences in RFI were observed between herds, showing that the weight gain from selection for growth did not cause changes in the energy efficiency of the animals.

  17. Performance and carcass traits in the diallel crossing of pacu and tambaqui

    Directory of Open Access Journals (Sweden)

    Rafael Vilhena Reis Neto

    2012-12-01

    Full Text Available With the purpose of evaluating the changes and the degree of heterosis on performance and carcass traits of fish from the diallel crossing between Pacu and Tambaqui, four genetic groups were generated through hormonal induction: pacu, tambaqui, tambacu and paqui. For 196 days, 400 juveniles of each genetic group were stocked in a pond divided into four parts of equal size, one for each group. At the end of this period, when the fish weighed on average 1024 g (±115.52 g, 20 specimens of each genetic group were sampled, weighed, measured: head length; standard length; body height; and width; and also processed resulting in following body yield: viscera; carcass; head; fillet; rib; residue and skin yields. There was positive heterosis for body weight, viscera and skin yield and head measures and negative heterosis for carcass and filet yield and body width, indicating that the hybrids have better performance on weight gain, but are inferior in relation to the carcass conformation.

  18. Genetic parameter estimates for carcass and yearling ultrasound measurements in Brangus cattle.

    Science.gov (United States)

    Moser, D W; Bertrand, J K; Misztal, I; Kriese, L A; Benyshek, L L

    1998-10-01

    Carcass measurements of 12th-rib fat thickness (CARCFAT), longissimus muscle area (CARCLMA), and weight (CARCWT) on 2,028 Brangus and Brangus-sired fed steers and heifers, as well as yearling weights (YWT) and ultrasound measures of 12th-rib fat thickness (USFAT) and longissimus muscle area (USLMA) on 3,583 Brangus bulls and heifers were analyzed to estimate genetic parameters. Data were analyzed using a six-trait animal model and an average information REML algorithm. The model included fixed effects for contemporary group and breed of dam, covariates for age at slaughter or measurement, and random animal and residual effects. Heritabilities for CARCFAT, CARCLMA, CARCWT, USFAT, USLMA, and YWT were .27+/-.05, .39+/-.05, .59+/-.06, .11+/-.03, .29+/-.04, and .40+/-.04, respectively. Genetic correlations between CARCFAT and USFAT, CARCLMA and USLMA, and CARCWT and YWT were .69+/-.18, .66+/-.14, and .61+/-.11, respectively. The favorable and moderately strong genetic correlations between carcass measurements and similar yearling breeding-animal ultrasound measurements indicate that such measurements of 12th-rib fat and longissimus muscle area are useful in predicting genetic values for carcass leanness and longissimus muscle area. Selection using yearling ultrasound measurements of breeding cattle should result in predictable genetic improvement for carcass characteristics. Inclusion of yearling ultrasound measurements for fat thickness and longissimus muscle area should enhance national cattle evaluation programs. PMID:9814892

  19. Classification of Pig Carcasses in Romania between 2007-2008

    Directory of Open Access Journals (Sweden)

    Diana Marin

    2010-10-01

    Full Text Available The classification of pig carcasses aims at a correct payment for big breeders, depending on carcass weight and quality, and standardization, the common language of the international meat trade. The European Union set uniform procedures of quality assessment (the EUROP system, defined by the same parameters in the entire continent. The pig livestock whose carcasses were classified in 2008 was almost 2.5 million, compared to the value below 2 million carcasses classified in 2007. Of the total livestock, in 2008, about 98% could be classified in the superior quality classes E and U. In the previous year, only 94% of the classified carcasses could be introduced into these superior quality classes. The increase of 4%, related to the number of 2.5 millions corresponding to 2008, represent more than 97.000 pigs whose carcasses belong to the superior classes, compared with the previous year.

  20. Influence of immunocastration (Bopriva® in weight gain, carcass characteristics and meat quality of Nellore

    Directory of Open Access Journals (Sweden)

    Nayara Andreo

    2014-02-01

    Full Text Available The objective of this study was evaluate the effects of immunocastration on body weight gain, carcass characteristics and meat quality of Nellore beef cattle. Eighty Nellore beef cattle, with initial body weight of 357±8.63 kg, were placed in feedlots and distributed in two treatments (40 animals per treatments as follow: one - non-vaccinated bulls and two - immunocastrated bulls (Bopriva®, Pfizer Animal Health. The animals placed on treatment two were vaccinated in two doses, first application 30 days before they arrive on the feedlots and second on the day they arrive on feedlots. After 67 days of experimental period, was calculated the daily gain of live weight in kg/day of the 80 animals and selected 20 animals from each treatment for the slaughter and carcass evaluations, and ten from each group for the meat analyzes. The data were submitted to analysis of variance. Immunocastrated animals showed lower daily weight gain, hot carcass weight, carcass yield, pH, leg thickness, muscle depth, loin muscle area, carcass muscle percentage, shear force and moisture. However, this animals had higher concentration of lactate and cortisol blood, chest depth, fat thickness, finishing degree, a *, b * and c*, liquid loss in thawing process, myofibrillar fragmentation index and ether extract of meat when compared to non-vaccinated bulss. The immunocastration (Bopriva® is an alternative for improving the quality of meat by the higher fat deposition and by the reduction of the shear force of the meat when compared to non-vaccinated bulls.

  1. Veterinary Microbiology, 3rd Edition

    Science.gov (United States)

    Veterinary Microbiology, Third Edition is organized into four sections and begins with an updated and expanded introductory section on infectious disease pathogenesis, diagnosis and clinical management. The second section covers bacterial and fungal pathogens, and the third section describes viral d...

  2. Soil Microbiology, Ecology, and Biochemistry

    Science.gov (United States)

    The 4th edition of Soil Microbiology, Ecology, and Biochemistry Edited by Eldor Paul continues in the vein of the 3rd edition by providing an excellent, broad-reaching introduction to soil biology. The new edition improves on the previous by providing extensive supplementary materials, links to outs...

  3. Microbiological Principles in Food Irradiation

    International Nuclear Information System (INIS)

    This paper reviews the important microbiological objectives of irradiation treatments, with special reference to the definitions of the proposed new terms, radappertization, radicidation and radurization. Emphasis is placed on the nature of the food in determining the microbiological requirements of the irradiation treatment. It is suggested that, just as with heat-processed foods, classifications into the major groups of ''acid'' or ''cured'' foods will remain valid with the irradiation process, and that different microbiological criteria will apply to these different classes of foods. The differences depend in part on the influence which the nature of the food has on the effectiveness of the irradiation treatment itself, but more especially on the way in which the nature of the food affects the activities of those microorganisms which might survive irradiation. The principles used to calculate the appropriate doses of radiation are discussed, with comments on the reliability of the fundamental assumptions or the need for further experimentation. The microbiological characteristics of irradiated foods are compared with those of corresponding heat- processed foods, to emphasize points of difference, with special reference to the appropriateness of suggested classifications for heat-processed foods. Finally, some general difficulties are considered, such as uncertainty about the significance and behaviour of food-borne viruses, and about the significance of the mutations which might conceivably be induced in microorganisms surviving-an irradiation process. (author)

  4. Respiratory virology and microbiology in intensive care units

    DEFF Research Database (Denmark)

    Østby, Anne-Cathrine; Gubbels, Sophie; Baake, Gerben;

    2013-01-01

    Our aim was to determine the frequency of 12 common respiratory viruses in patients admitted to intensive care units with respiratory symptoms, evaluate the clinical characteristics and to compare the results to routine microbiological diagnostics. Throat swabs from 122 intensive care-patients >18...

  5. [Microbiological quality of street-vendor ice cream in Dakar].

    Science.gov (United States)

    Aïdara-Kane, A; Ranaivo, A; Spiegel, A; Catteau, M; Rocourt, J

    2000-01-01

    During a multicenter study initiated by the International Network of Pasteur Institutes and Associated Institutes, microbiological quality of street-vended ice creams in Dakar was evaluated. 313 samples of ice creams from 170 street-vendors were collected and tested for common foodborne pathogens and indicator organisms. Results showed that microbiological quality of 45% of tested samples was unsatisfactory because of large populations of aerobic mesophilic organisms (36.7%), thermotolerant coliforms bacteria (21.4%) and sometimes E. coli. (10.6%). Strict pathogens as Salmonella, Shigella and Vibrio cholerae were not found. An investigation conducted among vendors showed a lack of education and training; these vendors need information about food preparation and storage practices that reduce microbiological contamination of foods. PMID:14666784

  6. SIRE EFFECTS ON CARCASS TRAITS OF JAPANESE BROWN COW

    OpenAIRE

    Sri Rachma, Aprilita Bugiwati

    2005-01-01

    The present research aims to obtain more fundamental knowledge of genetic effect (sire effect) on carcass traits of Japanese Brown cow. This experiment was done at Kumamoto Prefecture Japan. The field data of ultrasonic estimates of carcass traits of 9468 head of Japanese Brown cow, which had born from January 3rd 1988 to December 25th 1993 and representing by 88 head of sires were collected. All data were included of pedigree status. Cows data of ultrasonic estimates of carcass traits wer...

  7. Breed effects and retained heterosis for growth, carcass, and meat traits in advanced generations of composite populations of beef cattle.

    Science.gov (United States)

    Gregory, K E; Cundiff, L V; Koch, R M; Dikeman, M E; Koohmaraie, M

    1994-04-01

    Retained heterosis for growth, carcass, and meat traits was estimated in F3 generation castrate male progeny in three composite populations finished on two levels of dietary energy density (2.82 Mcal of ME and 3.07 Mcal of ME and 11.50% CP) and serially slaughtered at four end points at intervals of 20 to 22 d. Breed effects were evaluated in nine parental breeds (Red Poll [R], Hereford [H], Angus [A], Limousin [L], Braunvieh [B], Pinzgauer [P], Gelbvieh [G], Simmental [S], and Charolais [C] that contributed to the three composite populations (MARC I = 1/4 B, 1/4 C, 1/4 L, 1/8 H, 1/8 A; MARC II = 1/4 G, 1/4 S, 1/4 H, 1/4 A; and MARC III = 1/4 R, 1/4 P, 1/4 H, and 1/4 A). Breed effects were important (P .05) from Limousin in retail product weight or carcass lean weight because of higher dressing percentage, lower fat trim percentage, and lower bone percentage of Limousin. The effects of dietary energy density were important (P < .01) for most traits. The interaction of breed group x dietary energy density generally was not important. Retained heterosis generally was significant for each composite population for weight of retail product, fat trim, bone, and carcass lean, fat, and bone. For percentage of retail product, fat trim, carcass lean, carcass fat, and chemical fat in the 9-10-11th rib cut, generally, heterosis was significant for composites MARC II and MARC III but not for composite MARC I (i.e., composites MARC II and MARC III had a lower percentage of retail product and carcass lean and a higher percentage of fat trim, carcass fat, and chemical fat in the 9-10-11th rib cut than the mean of contributing purebreds).

  8. Postweaning growth and carcass traits in crossbred cattle from Hereford, Angus, Brangus, Beefmaster, Bonsmara, and Romosinuano maternal grandsires.

    Science.gov (United States)

    Casas, E; Thallman, R M; Kuehn, L A; Cundiff, L V

    2010-01-01

    The objective of this study was to characterize breeds representing diverse biological types for postweaning growth and carcass composition traits in terminal crossbred cattle. Postweaning growth and carcass traits were analyzed on 464 steers and 439 heifers obtained by mating F(1) cows to Charolais and MARC III (1/4 Hereford, 1/4 Angus, 1/4 Pinzgauer, and 1/4 Red Poll) sires. The F(1) cows were obtained from mating Angus and MARC III dams to Hereford, Angus, Beefmaster, Brangus, Bonsmara, and Romosinuano sires. Traits evaluated were postweaning ADG, slaughter weight, HCW, dressing percentage, percentage of carcasses classified as USDA Choice, LM area, marbling score, USDA yield grade, fat thickness, retail product yield (percentage), and retail product weight. Maternal grandsire breed was significant (P carcass weights, the least percentage of carcasses classified as USDA Choice, and the least amount of marbling and fat thickness. Animals with inheritance from these 2 breeds had a more desirable yield grade with the greatest retail product yield. Maternal granddam breed was significant (P < 0.05) for marbling score, USDA yield grade, fat thickness, and retail product yield. Sex class was significant (P < 0.05) for all traits except for retail product yield. Steers grew faster, were heavier, had heavier carcasses, and were leaner than heifers. Heifers had a greater dressing percentage, a greater percentage of carcasses classified as USDA Choice, a greater LM area, and a decreased yield grade when compared with steers. Sire and grandsire breed effects can be optimized by selection and use of appropriate crossbreeding systems.

  9. Characterization of two Pro-opiomelanocortin gene variants and their effects on carcass traits in beef cattle

    Directory of Open Access Journals (Sweden)

    Deobald Heather M

    2011-01-01

    Full Text Available Abstract Background Carcass quantity (lean meat yield and quality (degree of marbling in beef cattle determines much of their economic value. Consequently, it is important to study genes that are part of the appetite pathway and that may ultimately affect carcass composition. Pro-opiomelanocortin is a prohormone that codes for many different peptides, several of which are involved in the appetite pathway. A single nucleotide polymorphism (SNP c.288C>T in pro-opiomelanocortin (POMC has previously been associated with hot carcass weight (HCW and shipping weight (Ship wt in beef cattle. Results While developing a commercial real time PCR test for POMC c.288C>T a 12 bp deletion (POMC c.293_304delTTGGGGGCGCGG was identified. The deletion results in the removal of four amino acids (a valine, two glycines, and an alanine. Both the POMC c.288C>T and the deletion were genotyped in 386 crossbred steers and evaluated for associations with carcass traits. The animals with one copy of the deletion had a significantly smaller carcass rib-eye area (7.91 cm2; P = 0.02 in comparison to homozygous normal animals. Significant associations were observed between POMC c.288C>T with start-of-finishing weight (SOF WT; P = 0.04, hot carcass weight (HCW; P = 0.02, average fat and grade fat (both P = 0.05, carcass rib-eye area (REA; P = 0.03 and marbling (P = 0.02. Conclusions These results suggest that it could be beneficial for beef producers to know both the deletion and POMC c.288C>T genotypes when making marketing and culling decisions.

  10. Performance, carcass characteristics and gain cost of feedlot cattle fed a high level of concentrate and different feed additives

    Directory of Open Access Journals (Sweden)

    Érico Rodrigues

    2013-01-01

    Full Text Available The objective of this study was to evaluate the effects of feeding cattle with isoprotein and isoenergetic diets, with and without the addition of polyclonal antibody preparation (PAP, yeasts (YST or monensin sodium (MON on performance, carcass characteristics and gain cost in feedlot. Ninety-five 20-month old bullocks (323.3±21.8 kg were distributed in 25 pens. The completely randomized experimental design had a 2 × 2 + 1 factorial arrangement and the treatments were replicated 5 times. There was no effect of MON for DMI throughout the feedlot period; however, MON reduced the dry matter intake (DMI in g/kg of BW in the first 28 days when compared with the other treatments. The gain cost decreased with MON addition in relation to the other treatments. Inclusion of YST decreased average daily gain (ADG, final body weight, hot carcass weight, carcass weight, gain to feed ratio and DMI in g/kg body weight, worsening feed conversion and increasing the gain cost in the feeding periods. Inclusion of PAP increased ADG and decreased the gain cost, besides improving feed conversion. For MON and PAP, a difference was found for kidney-pelvic fat and kidney-pelvic fat per 100 kg of hot carcass weight. For MON and YST, there was a difference in ADG, feed conversion, gain cost and carcass yield and kidney-pelvic fat per 100 kg of hot carcass. Treatment YST worsened performance in relation to the non-supplemented treatments. Feeding PAP to animals did not influence performance and carcass characteristics of bullocks in feedlot negatively. Thus, PAP shows potential to substitute MON in cattle feeding using isoprotein and isoenergetic diets.

  11. PRICE DEVELOPMENTS OF BEEF CARCASSES IN ROMANIA AND EUROPEAN UNION

    Directory of Open Access Journals (Sweden)

    IOANA BĂLAN

    2013-07-01

    Full Text Available The main objectives of the classification of cattle carcasses (beef and veal are represented by the correct payment to cattle breeders, according to carcass weight and quality, and standardization, the common language in the international meat trade. The European Union set uniform procedures for quality assessment (the EUROP system, defined by the same parameters in the whole continent. Romania is at the beginning of cattle carcass classification; it is in the third year of reporting on the classification results to the European Commission. In this context, we consider it is very important to analyze these results, for a continuous improvement of carcass quality.

  12. ESCHERICHIA COLI O157 IN WITHE VEAL CALVES CARCASSES

    OpenAIRE

    V. D’Orio; M. Conter; G. Colavita; M. Paoletti

    2013-01-01

    During 2006 one hundred and three white veal calves slaughtered at an abattoir in Isernia (Italy) were examined for E. coli O157 intestinal carriage and carcass contamination, using Immuno-Magnetic Separation (IMS) and multiplex PCR. Faecal material before slaughtering and carcass samples, using sponge-bag, were collected. E. coli O157 isolates were found in 10 (9,71%) faecal and 9 (8,7%) carcass samples. PCR analysis showed that all the strains from faecal and from carcass samples carried ea...

  13. PREDICTION OF CARCASS TRAITS OF JAPANESE BLACK BULLS AT SEVERAL AGES USING BODY MEASUREMENTS AND ULTRASONIC ESTIMATE OF CARCASS TRAITS

    OpenAIRE

    Sri Rachma, Aprilita Bugiwati

    2008-01-01

    The present research aims to estimate the mathematical equations for predicting ultrasonic estimates of carcass traits at ten months after performance test (about 20 months of age) using body measurements and ultrasonic estimates of carcass traits at earlier stages of performance test of Japanese Black bulls. This research was done at Kagoshima and Miyazaki prefecture Livestock Experimental Stations Japan to collect the ultrasonic estimates of carcass traits and body measurements data of Jap...

  14. Prevalence and Serogroup Diversity of Salmonella for Broiler Neck Skin, Whole Carcass Rinse, and Whole Carcass Enrichment Sampling Methodologies following Air or Immersion Chilling.

    Science.gov (United States)

    Bourassa, D V; Holmes, J M; Cason, J A; Cox, N A; Rigsby, L L; Buhr, R J

    2015-11-01

    The purpose of this study was to evaluate neck skin (NS), whole carcass rinse (WCR), and whole carcass enrichment (WCE) sampling procedures for Salmonella isolation and serogroup identification from the same broiler chicken carcass treated with air or immersion chilling. Commercially processed and eviscerated broiler carcasses were collected from a commercial processing plant, individually bagged, and transported to the pilot processing plant. In experiment 1, carcasses were air chilled to 4°C. In experiment 2, carcasses were immersion chilled with or without chlorine. After air chilling, Salmonella was detected on 78% of NS and 89% of WCE samples. Only one Salmonella serogroup was detected from each of 13 Salmonella-positive NS samples, and two serogroups were detected on 1 Salmonella-positive NS sample. Only one Salmonella serogroup was detected from each of 13 Salmonella-positive WCE samples, and two serogroups were detected from 3 Salmonella-positive WCE samples. After immersion chilling without chlorine, Salmonella was detected on 38% of NS, 45% of WCR, and 100% of WCE samples. Without chlorine, the 15 Salmonella-positive NS samples included 14 samples with one serogroup and 1 sample with two serogroups. Only one Salmonella serogroup was detected from WCR samples after immersion chilling. Of 40 Salmonella-positive WCE samples, 23 had a one, 14 had two, and 3 had three Salmonella serogroups. After immersion chilling with chlorine, Salmonella was detected on 35% of NS, 0% of WCR, and 90% of WCE samples. With chlorine, the 14 Salmonella-positive NS samples included 11 samples with one serogroup and 3 samples with two serogroups. No Salmonella serogroups were detected from WCR samples after immersion chilling with 20 mg/liter free chlorine. The 36 Salmonella-positive WCE samples included 21 samples with one serogroup and 15 samples with two serogroups. NS and WCE sampling methodologies yielded similar prevalence and serogroup diversity after air chilling. However

  15. Carcass physical composition and meat quality of Charolais cattle of different categories

    Directory of Open Access Journals (Sweden)

    Paulo Santana Pacheco

    2011-11-01

    Full Text Available The objective of the experiment was to study the carcass physical composition and meat qualitative characteristics of different categories of Charolais cattle, feedlot finished. Nine steers (ST, ten heifers (HE, with average age of 20 months, and ten cull cows (CO with average age of 87 months, taken from the same herd, were used. Diet contained 13% of crude protein for ST and HE and 10% for CO, with roughage:concentrate relation of 50:50, on a dry matter basis. The animals of the three categories were slaughtered with similar degree of finishing, with average subcutaneous fat thickness of 3.8 mm. HE showed carcasses with greater pistolcut percentage (52.8% than ST (51.0% and CO (51.4%. The carcass physical composition was similar among the categories evaluated, with 66.5; 67.0 and 67.3% of muscle, 19.1; 18.5 and 18.7% of fat, and 14.9; 15.0; and 14.6% of bone, respectively, for ST, HE and CO. The marbling score was lower for HE (4.6 points than ST (7.1 points and CO (6.5 points. However, when this characteristic was adjusted for 100 kg of cold carcass, the means were similar among the categories. CO showed higher cooking losses (27.8% than ST (21.4% and HE (23.5%. Meat color, texture, tenderness, shear force, palatability and juiciness were similar among the categories. Charolais cows have the potential for the production of carcass and meat with quality similar to steers and heifers.

  16. Growth, puberty, and carcass characteristics of Brahman-, Senepol-, and Tuli-sired F1 Angus bulls.

    Science.gov (United States)

    Chase, C C; Chenoweth, P J; Larsen, R E; Hammond, A C; Olson, T A; West, R L; Johnson, D D

    2001-08-01

    Postweaning growth, sexual development, libido, and carcass data were collected from two consecutive calf crops using 31 Brahman x Angus (B x A), 41 Senepol x Angus (S x A), and 38 Tuli x Angus (T x A) F1 bulls. Following weaning (by mid-September) and preconditioning, at the start of the study (late September) bulls were fed concentrate (three times each week at a rate equivalent to 4.5 kg/d) on bahiagrass pasture for approximately 250 d. At the start of the study and at 28-d intervals, BW, hip height, and scrotal circumference (SC) were measured. Concurrently at 28-d intervals, when the SC of a bull was > or = 23 cm, semen collection was attempted using electroejaculation. Ejaculates were evaluated for presence of first spermatozoa (FS), 50 x 10(6) sperm with at least 10% motility (PU), and 500 x 10(6) sperm with at least 50% motility (PP). After all bulls reached PP they were subjected to two libido tests. Carcass data were collected on all bulls (n = 110) and Warner-Bratzler shear (WBS) force values were assessed on a subset (n = 80). For both years, B x A bulls were heavier (P 0.10) gain in BW or hip height during the study. Scrotal circumference of T x A bulls was larger (P 0.10) of breed type by the end of the study. At PU and PP, B x A bulls were older (P carcass traits; B x A bulls had the heaviest (P carcass weight, greatest (P 0.10) USDA quality grade. In conclusion, tropically adapted F1 bulls produced from Senepol (Bos taurus) and Tuli (Sanga) sires bred to Angus cows in Florida had lighter BW, shorter hip heights, and smaller carcasses than those from Brahman sires but reached puberty earlier and had higher libido scores and lower WBS force values.

  17. Carcass Composition and Cuts of Bulls and Steers Fed with Three Concentrate Levels in the Diets.

    Science.gov (United States)

    do Prado, Ivanor Nunes; Passetti, Rodrigo Augusto Cortêz; Rivaroli, Dayane Cristina; Ornaghi, Mariana Garcia; de Souza, Kennyson Alves; Carvalho, Camila Barbosa; Perotto, Daniel; Moletta, José Luiz

    2015-09-01

    In this paper, weight, carcass dressing, weights of the primary cuts, weights of the physical components of the primary cuts, and weights of the main commercial cuts of 66 Purunã animals, of which 33 were bulls and 33 were steers were evaluated. These animals, with an average age of 19 months at the beginning of the experiment, were finished in a feedlot system during 116 days, and were fed with diets containing three levels of concentrate (0.8%, 1.1%, and 1.4% of body weight). The concentrate was formulated with 25% soybean meal, 73% ground corn grain, 1% of a mineral mix, and 1% of limestone. The interaction between sexual groups and the concentrate level was not significant for any of the variables. Likewise, no effect of the concentrate level was detected on the same variable traits. The bulls demonstrated higher hot carcass weights (265.1 vs 221.7 kg) and a higher proportion of forequarter (38.4% vs 36.1%) however the steers presented with higher proportions of side (19.7% vs 18.5%) and hindquarter (44.2% vs 43.1%). The bulls produced higher yields of muscle in the three primary cuts, there by resulting in higher yields of edible portions of the carcass. The bulls also produced higher weights of tenderloin, knuckle, topside, flat, eye round, rump, and rump cover. The finishing of young bulls in feedlot is to be recommended, since the animals produce carcasses with higher amounts of edible meat and higher yields of commercial cuts, thus allowing for a better price for the carcass. Low concentrate level could be used due to the lower cost of production for farmers. PMID:26194216

  18. Carcass traits and meat quality of two different rabbit genotypes

    Directory of Open Access Journals (Sweden)

    Maria D'Agata

    2012-07-01

    Full Text Available To evaluate the effect of genotype on carcass traits and meat quality, thirty-two rabbits for two genotypes (local population – LP; commercial hybrids – HY were used. Rabbits were weaned at 35 days old and slaughtered at 103 days of age for LP and 87 days of age for HY. Comparing the slaughtering traits of two genotypes, LP provided higher dressing out (59.4% vs 56.2%, Pvs 14.2%, Pvs 22.3, Pvs 8,9%, Pvs 0.86%, Pvs 1.12%; Pvs 19.2%; Pvs 31.6%; Pvs 3.8%; P*, higher redness (a*, yellowness (b* and C* value than HY (P

  19. Avaliação das proporções dos cortes da carcaça, características da carne e avaliação dos componentes do peso vivo de cordeiros Evaluation of carcass cuttings proportion, meat characteristics and evaluation of live weight components of lambs

    Directory of Open Access Journals (Sweden)

    Rafael Batista Medeiros Frescura

    2005-02-01

    Full Text Available Objetivou-se, neste trabalho, avaliar a composição física da carcaça, as características da carne e a proporção dos não-componentes da carcaça do peso vivo de cordeiros abatidos aos 28 kg submetidos a diferentes sistemas alimentares. Dezoito cordeiros (Ile de France x Texel foram distribuídos aleatoriamente, com as respectivas mães, em três tratamentos: PCA - pastagem cultivada de azevém (Lolium multiflorum Lam., CON - confinamento sem alimentação privativa e CCF - confinamento com alimentação privativa para os cordeiros. A alimentação privativa foi oferecida aos cordeiros pelo sistema de creep feeding. Não houve diferença para o percentual do pescoço entre PCA, CON e CCF, com valores de 9,27; 9,17 e 8,72%, respectivamente. Para o percentual de perna, os animais da CON (34,02% e CCF (34,17% apresentaram valores semelhantes entre si e superiores aos da PCA (31,73%. A maciez medida na porção do Longissimus dorsi entre a 9ª e 12ª costelas foi semelhante entre os animais dos três tratamentos, de 2,33; 3,03 e 3,08 para PCA, CON e CCF, respectivamente. Não houve efeito dos tratamentos sobre a palatabilidade e suculência da carne. O percentual de pele dos animais da PCA (11,05% e do CON (10,50% foram semelhantes entre si, enquanto o PCA apresentou valores mais elevados que o percentual dos animais do CCF (9,70%. Para o percentual do conteúdo gástrico, os valores observados para os animais do CCF (11,47% foram superiores àqueles da PCA (5,09% e semelhantes aos obtidos para os animais do CON (8,72%.This study aimed to evaluate carcass physical comp0sition, meat characteristics and live weight carcass no component of lambs slaughter at 28 kg under different feeding systems. Eighteen lambs (Ile de France X Texel were randomly allotted, with their respective mothers, to three treatments: RP - ryegrass pasture (Lolium multiflorum Lam., COM - confinament of lamb and sheep together and CCF - confinament with creep feeding. A

  20. Postweaning growth and carcass traits in crossbred cattle from Hereford, Angus, Brangus, Beefmaster, Bonsmara, and Romosinuano maternal grandsires.

    Science.gov (United States)

    Casas, E; Thallman, R M; Kuehn, L A; Cundiff, L V

    2010-01-01

    The objective of this study was to characterize breeds representing diverse biological types for postweaning growth and carcass composition traits in terminal crossbred cattle. Postweaning growth and carcass traits were analyzed on 464 steers and 439 heifers obtained by mating F(1) cows to Charolais and MARC III (1/4 Hereford, 1/4 Angus, 1/4 Pinzgauer, and 1/4 Red Poll) sires. The F(1) cows were obtained from mating Angus and MARC III dams to Hereford, Angus, Beefmaster, Brangus, Bonsmara, and Romosinuano sires. Traits evaluated were postweaning ADG, slaughter weight, HCW, dressing percentage, percentage of carcasses classified as USDA Choice, LM area, marbling score, USDA yield grade, fat thickness, retail product yield (percentage), and retail product weight. Maternal grandsire breed was significant (P yield grade with the greatest retail product yield. Maternal granddam breed was significant (P yield grade, fat thickness, and retail product yield. Sex class was significant (P yield. Steers grew faster, were heavier, had heavier carcasses, and were leaner than heifers. Heifers had a greater dressing percentage, a greater percentage of carcasses classified as USDA Choice, a greater LM area, and a decreased yield grade when compared with steers. Sire and grandsire breed effects can be optimized by selection and use of appropriate crossbreeding systems. PMID:19820064

  1. Broiler chicken PSE (Pale, Soft, Exudative meat and water release during chicken carcass thawing and brazilian legislation

    Directory of Open Access Journals (Sweden)

    Talita Kato

    2013-12-01

    Full Text Available The objective of this work was to investigate the relationship between poultry PSE meat and water loss of frozen chicken carcass in two experiments. The first experiment was carried out in commercial abattoir. Poultry carcass were classified as PSE meat (n=59 (pH 5.8. Water absorption and drip test were performed according to the Brazilian legislation methodologies. The second experiment was carried out with commercial whole five brands frozen carcasses purchased from the local supermarkets (n=30 and analyzed for pH, water holding capacity (WHC and drip test. PSE poultry meats absorbed 3.59% of water during the processing similar to the control samples; however, released 0.38% more water during thawing of the carcasses. From these five brands evaluated, three presented drip values above 6.0%. The highest drip value was showed by the brand sample that had pH and WHC values characteristics of PSE meat. It could be postulated that PSE meat phenomenon promoted more water release during thawing leading to a misinterpretation in relation to the Brazilian legislation for water carcass liberation during thawing.

  2. Relationships between carcass characteristics, meat quality, age and sex of free-ranging Alaskan reindeer: a pilot study

    Directory of Open Access Journals (Sweden)

    Teresa A. Renecker

    2005-03-01

    Full Text Available Twenty-four reindeer (Rangifer tarandus carcasses from male and female animals that ranged in age from calves to adults were purchased from Bering Sea Reindeer Products (BSRP, Nunivak Island, Alaska, USA. Preslaughter and abattoir procedures were observed and evaluated. Carcasses were split in half, weighed, and broke into wholesale primal cuts of chuck, rib, loin, and hindquarter. Each primal cut was weighed, boxed, and frozen. Each half carcass of primal cuts was later dissected into lean tissue, bone, and the three compartments of fat: subcutaneous, intermuscular, and peritoneal. A portion of the loin was collected from each animal in order to obtain data on pH and shear force. Sensory panel analysis was performed on loin steaks. Due to management and environmental effects, pH values were high and the meat was dark in colour. Carcasses from adult male reindeer contained significantly lower levels of fat than carcasses of adult females. Data indicated that yearling reindeer are of greatest economic value for meat production.

  3. Effect of fermented noni leaf (Morinda citrifolia L. in diets on cholesterol content of broiler chicken carcass

    Directory of Open Access Journals (Sweden)

    Erman Syahruddin

    2011-12-01

    Full Text Available Chicken meat is very nutritious. It is sometimes blamed to cause strock attack and coronary heart disease in human, because of high fat and cholesterol contents in the chicken meat. Therefore, the aim of this experiment is to evaluate the effect of fermented noni leaf levels in diets on the cholesterol content of broiler chicken carcass. The experiment was based on completely randomized design with eight experimental diets containing 0, 3, 6, 9, 12, 15, 18 and 21% of fermented noni leaf powder. All diets were formulated to contain 22% crude protein and 3000 kcal/kg. Each treatment had three replicates with ten chickens per replicate. Two hundred and forty day old unsex broiler chicks Arbor Acress were fed ad lib. for eight weeks and then sacrificed. Feed consumption, body weight gain, feed conversion ratio, and cholesterol content of carcass were taken as variable responses. Data were analyzed based on analysis of variance and orthogonal comparisons. Results showed that feed consumption, daily weight gain, FCR and carcass content were not affected by the levels of fermented noni leaf in the diet. However, cholesterol content of broiler carcass was significantly (P < 0.05 affected by the dietary treatments. Cholesterol content of the carcass was reduced processed 26.18% 73.06 to 53.76 mg/100g mg/100g chicken meat. The lowest cholesterol level was obtained by feeding the chickens with diets containing 21% fermented noni leaf.

  4. Evaluation of the efficacy of a probiotic drink containing Lactobacillus casei on the levels of periodontopathic bacteria in periodontitis: A clinico-microbiologic study

    Directory of Open Access Journals (Sweden)

    Faizuddin Imran

    2015-01-01

    Full Text Available Background and Objectives: This study was designed to evaluate whether the oral administration of lactobacilli could change the bacterial population in subgingival plaque. Subjects and Methods: Forty-two healthy volunteers with chronic generalized mild to moderate periodontitis were given a probiotic drink containing Lactobacillus casei for 1 month. Subgingival plaque samples were collected at baseline, after which the patients were asked to consume the probiotic drink once daily for 1 month. At the 1 month interval, plaque samples were collected, and the drink discontinued. The patients were recalled at 2 months interval for collection of the final samples. The bacterial amounts in the plaque samples were analyzed by multiplex polymerase chain reaction procedure. Results: Of the three periodontopathic bacteria selected, Porphyromonas gingivalis showed highly significant reductions in the bacterial levels at 1-month and 2 months intervals. In comparison, Aggregatibacter actinomycetemcomitans, when present higher than 10 × 10 3 at baseline, and Prevotella intermedia present higher than 2 × 10 3 at baseline, showed moderately significant reduction in their numbers. Interpretation and Conclusion: Oral administration of the probiotic lactobacilli reduced the numerical sum of the three selected periodontopathic bacteria and could contribute to the beneficial effects on periodontal conditions.

  5. Microbiological Spoilage of Cereal Products

    Science.gov (United States)

    Cook, Frederick K.; Johnson, Billie L.

    A wide range of cereal products, including bakery items, refrigerated dough, fresh pasta products, dried cereal products, snack foods, and bakery mixes, are manufactured for food consumption. These products are subject to physical, chemical, and microbiological spoilage that affects the taste, aroma, leavening, appearance, and overall quality of the end consumer product. Microorganisms are ubiquitous in nature and have the potential for causing food spoilage and foodborne disease. However, compared to other categories of food products, bakery products rarely cause food poisoning. The heat that is applied during baking or frying usually eliminates pathogenic and spoilage microorganisms, and low moisture contributes to product stability. Nevertheless, microbiological spoilage of these products occurs, resulting in substantial economic losses.

  6. Microbiological testing of Skylab foods.

    Science.gov (United States)

    Heidelbaugh, N. D.; Mcqueen, J. L.; Rowley, D. B.; Powers , E. M.; Bourland, C. T.

    1973-01-01

    Review of some of the unique food microbiology problems and problem-generating circumstances the Skylab manned space flight program involves. The situations these problems arise from include: extended storage times, variations in storage temperatures, no opportunity to resupply or change foods after launch of the Skylab Workshop, first use of frozen foods in space, first use of a food-warming device in weightlessness, relatively small size of production lots requiring statistically valid sampling plans, and use of food as an accurately controlled part in a set of sophisticated life science experiments. Consideration of all of these situations produced the need for definite microbiological tests and test limits. These tests are described along with the rationale for their selection. Reported test results show good compliance with the test limits.

  7. Microbiology and Human Spaceflight Applications

    Science.gov (United States)

    Ott, C. Mark

    2016-01-01

    As humans continue to travel further into space, microorganisms will accompany them. Thus, understanding how microorganisms can impact the crew, the spacecraft, and spacecraft systems is critical to enable future spaceflight exploration. To mitigate microbial risks during spaceflight, NASA relies heavily on preventative measures, including appropriate vehicle design, crew quarantine prior to flight, and extensive microbial monitoring. While these precautions minimize the proliferation of infectious agents, their presence cannot be completely eliminated. Microbiological contamination of vehicle systems can also be a key issue for long duration missions, as system deterioration and fouling have been previously observed in spacecraft. Current studies of the microbiomes of the crew and the International Space Station environment are providing a wealth of information and hold the potential to help refine microbiological requirements for NASA mission beyond low Earth orbit, as spaceflight environments and mission architectures rapidly evolve.

  8. Tools for Microbiological risk assessment

    DEFF Research Database (Denmark)

    Bassett, john; Nauta, Maarten; Lindqvist, Roland;

    Microbiological Risk Assessment (MRA) has emerged as a comprehensive and systematic approach for addressing the risk of pathogens in specific foods and/or processes. At government level, MRA is increasingly recognised as a structured and objective approach to understand the level of risk in a given...... can increase the understanding of microbiological risks in foods. It is timely to inform food safety professionals about the availability and utility of MRA tools. Therefore, the focus of this report is to aid the food safety manager by providing a concise summary of the tools available for the MRA...... food/pathogen scenario. Tools developed so far support qualitative and quantitative assessments of the risk that a food pathogen poses to a particular population. Risk can be expressed as absolute numbers or as relative (ranked) risks. The food industry is beginning to appreciate that the tools for MRA...

  9. Microbiological evaluation of different reprocessing methods for cuffed and un-cuffed tracheostomy tubes in home-care and hospital setting

    Directory of Open Access Journals (Sweden)

    Leonhard, Matthias

    2016-02-01

    Full Text Available Background: Manufacturers’ recommendations on cleaning of tracheostomy tubes focus on general warning information and non-specific manual cleaning procedures. The aim of this experimental study was to evaluate different reprocessing methods and to determine the mechanical integrity and functionality of tracheostomy tubes following reprocessing.Methods: Sixteen cuffed or un-cuffed tracheostomy tubes obtained from hospital in-patients were reprocessed using one of the following reprocessing methods: a manual brushing and rinsing with tap water, brushing followed by disinfection with a glutaraldehyde solution, c manual brushing followed machine-based cleaning in a dishwasher, and d manual brushing followed by ultrasound cleaning in a commercially available ultrasound device. Microbial burden of the tubes before and after reprocessing was assessed by measurement of microbial colony-forming units per mL (CFU/mL of rinsing fluid. After cleaning, tracheostomy tubes were investigated for loss of functionality. Findings: Manual brushing and rinsing with tap water reduced microbial colonization in average by 10 CFU/mL, but with poor reproducibility and reliability. Complete microbial reduction was achieved only with additional chemical or machine-based thermal disinfection. Ultrasound sonification yielded no further microbial reduction after manual brushing. Conclusion: Manual brushing alone will not result in complete eradication of microorganism colonising cuffed or un-cuffed tracheostomy tubes. However, manual cleaning followed by chemical or thermal disinfection may be regarded as safe and reproducible reprocessing method. If a machine-based reprocessing method is used for cuffed tubes, the cuffs’ ventilation hose must be secured in a safe position prior to thermal disinfection.

  10. Microbiological quality of natural waters.

    Science.gov (United States)

    Borrego, J J; Figueras, M J

    1997-12-01

    Several aspects of the microbiological quality of natural waters, especially recreational waters, have been reviewed. The importance of the water as a vehicle and/or a reservoir of human pathogenic microorganisms is also discussed. In addition, the concepts, types and techniques of microbial indicator and index microorganisms are established. The most important differences between faecal streptococci and enterococci have been discussed, defining the concept and species included. In addition, we have revised the main alternative indicators used to measure the water quality.

  11. [Modified lateral condensation (microbiological analysis)].

    Science.gov (United States)

    Jácome Musule, J L; Vázquez del Mercado, M A; Hernández, J L; Granillo, N E

    1989-05-01

    In order to ascertain the presence of endodontobacterial flora, an in vivo microbiological study on fifty human teeth with necrotic pulp and periapical bone destruction, is submitted. The study verifies elimination of bacteria through a technique for the preparation of root canals. Results of this research ratify the presence of aerobic and anaerobic microorganisms, as well as the means to inhibit them. That will allow performing obturation under optimal conditions, thus increasing assurance of success in the endodontic therapy.

  12. Clinical, Radiological, Microbiological, and Histopathological Aspects of Acquired Dacryocystoceles

    Directory of Open Access Journals (Sweden)

    Selam Yekta Sendul

    2014-01-01

    Full Text Available Purpose. The aim of this study is to investigate the etiology and the clinical, microbiological, histopathological, and radiological findings of acquired dacryocystoceles. Methods. In this retrospective study, we reviewed the clinical records of 10 eyes of 8 patients with dacryocystoceles who underwent external dacryocystorhinostomy (DCR surgery. Etiology, presenting symptoms and radiological findings as well as microbiological and histopathological assessment results and outcome were analyzed. Results. The records of 8 patients with dacryocystoceles were included in this study. In the histopathological evaluations of the samples collected from the lacrimal sac wall, chronic inflammation was found in all biopsied samples and fibrosis was observed in two histopathological evaluations. Computerized tomography (CT imaging showed fluid collection separated from adjacent tissues by a thin rim, corresponding to dacryocystoceles in the sac. In the microbiological culture examination of samples collected from the fluid within the cyst, no bacterial growth in 5 eyes, gram-negative bacillus growth in 3 eyes, and gram-positive cocci growth in 2 eyes were found. Conclusions. Acquired dacryocystoceles were observed extremely rarely and a definite pathogenic agent could not be identified in any of the cases, either microbiologically or histologically, whereas chronic inflammation was detected in all cases in our study.

  13. Microbiological and Sensory Quality of Milk on the Domestic Market

    Directory of Open Access Journals (Sweden)

    Piotrowska Anna

    2015-12-01

    Full Text Available The evaluation of the microbiological and sensory quality of 30 assortments of market milk was the objective of the presented research. The study also compared the sensory profile of selected samples of milk differing in preservation method. Microbiological tests included determination of the presence and number of Enterobacteriaceae and the total number of microorganisms. Sensory characteristics of all the milk samples was performed by scaling method (5-points scale. Selected samples of milk were additionally assessed by means of profile method. Microbiological tests showed satisfactory microbiological purity of milk, which indicates the effectiveness of the heat treatment and the lack of secondary contamination. Regardless of the preservation method, all the evaluated milk samples were characterised by a high sensory quality (4.25-5 points. There were no changes in smell and taste, resulting from the development of undesirable microflora. The tested samples of milk differing in the preservation method (sterilisation, pasteurisation at high temperature and subjected to microfiltration and low pasteurisation varied in the sensory profile. Milk pasteurised at high temperature was characterised by the most harmonised overall quality.

  14. Microbiological aspects of endoperiodontal lesion

    Directory of Open Access Journals (Sweden)

    Cristiane Tokunaga

    2013-06-01

    Full Text Available Introduction: The endoperiodontal lesion occurs when a tooth undergoing endodontic disease is united to a periodontal lesion with apical progression. Many times, the differential diagnosis between the endodontic and periodontal disease can be of difficult execution and the correct diagnosis and planing of the treatment is of main importance for a good prognosis Objective: To identify the main microorganisms within the lesion of endodontic and periodontal origin and correlate them with the endoperiodontal lesion. Literature review: The search strategy comprised the electronic studies of databases such as PubMed and Cochrane on the microbiology of the endodontic and periodontal systems through employing the following keywords: microbiology, endodontics, periodontal pocket. Results: There were similarities in the endodontic and periodontal microflora. However, the number of microorganisms within the cross infection is limited, including Bacteroides, Eubacteria, Fusobacteria, spirochaetes, Wolinella. The bacterias forming the red complex are closely related to the severity of the periodontal disease and can also participate in the pathogenesis of the periradicular abscesses. Conclusion: There are many communication routes between the periodontium and pulpal tissue, therefore the contamination from um tissue to another can occur, existing a microbiological inter-relationship between these tissues.

  15. Copepod carcasses as microbial hot spots for pelagic denitrification

    DEFF Research Database (Denmark)

    Glud, Ronnie N.; Grossart, Hans-Peter; Larsen, Morten;

    2015-01-01

    Copepods are exposed to a high non-predatory mortality and their decomposing carcasses act as microniches with intensified microbial activity. Sinking carcasses could thereby represent anoxic microenvironment sustaining anaerobic microbial pathways in otherwise oxic water columns. Using non-invas...

  16. STEER AND HEIFER PRICE DIFFERENCES IN THE LIVE CATTLE AND CARCASS MARKETS

    OpenAIRE

    Schultz, Robert W.; Marsh, John M.

    1985-01-01

    A dynamic model is used to estimate quarterly price differences between steers and heifers in the feeder, slaughter, and carcass markets. For cattle within the same weight and grade range, their price differences are hypothesized to be influenced by seasonal, economic, and partly reflecting time changes in evaluation of steer and heifer quality in the live cattle and dressed meat trades. Stochastic factors are less prevalent at the feeder level, although risk of placing pregnant heifers in fe...

  17. Optical position detection to measure tyre carcass deflections and implementation for vehicle state estimation

    OpenAIRE

    Tuononen, Ari

    2009-01-01

    Future active safety systems for vehicles will need accurate information about the state of the vehicle. Tyre sensors certainly offer an interesting option to evaluate the operating state of an individual tyre. On the other hand, tyre behaviour in some driving conditions is not completely understood and tyre sensor measurements can contribute to the fundamentals of tyre research as well. This thesis discusses a method to measure the carcass deflection of a rolling tyre. Tyre force estima...

  18. Influence of immunocastration (Bopriva®) in weight gain, carcass characteristics and meat quality of Nellore

    OpenAIRE

    Nayara Andreo; Ana Maria Bridi; Marina Avena Tarsitano; Louise Manha Peres; Ana Paula Ayub da Costa Barbon; Evelyn Lopes de Andrade; Paulo Emílio Fernandes Prohmann

    2014-01-01

    The objective of this study was evaluate the effects of immunocastration on body weight gain, carcass characteristics and meat quality of Nellore beef cattle. Eighty Nellore beef cattle, with initial body weight of 357±8.63 kg, were placed in feedlots and distributed in two treatments (40 animals per treatments) as follow: one - non-vaccinated bulls and two - immunocastrated bulls (Bopriva®, Pfizer Animal Health). The animals placed on treatment two were vaccinated in two doses, first applica...

  19. Marbling classification of lambs carcasses with the artificial neural image analysis

    Science.gov (United States)

    Przybylak, A.; Ślósarz, P.; Boniecki, P.; Koszela, K.; Przybył, K.; Wojcieszak, D.; Szulc, R.; Ludwiczak, A.; Górna, K.

    2015-07-01

    This paper describes a part of research, whose goal was to develop an effective method to determine marbling classes of lamb carcasses, with the neural image analysis techniques. Current methods for identifying the degree of intramuscular fat level content are time consuming, require specialized expertise and often rely on subjective assessment based on predefined patterns. In this paper, authors proposes the use of neural model developed as a tool to assist evaluation of marbling.

  20. EFFECT OF TRANSPORT DURATION AND GENDER ON RABBIT CARCASS AND MEAT QUALITY

    OpenAIRE

    A. Trocino; Xiccato, G.; P.I Queaque; Sartori, A.

    2003-01-01

    In order to evaluate the effect of transport duration and gender on carcass and meat quality, 204 rabbits were reared in individual cages from 35 d of age until slaughter at 77 d (average live weight at shipping: 2662±189 g). Before slaughter, four groups of rabbits were formed and transported for 2, 4, 6, or 8 hours from the breeding unit to the slaughterhouse. Increasing the duration of transport increased transport losses (2.44% to 4.59%; P

  1. Carcass traits of Nellore, F1 Simmental × Nellore and F1 Angus × Nellore steers fed at maintenance or ad libitum with two concentrate levels in the diet

    Directory of Open Access Journals (Sweden)

    Ivanna Moraes de Oliveira

    2011-12-01

    Full Text Available This trial was conducted to evaluate the quantitative carcass traits of Nellore, F1 Simmental × Nellore and F1 Angus × Nellore steers fed at maintenance level or ad libitum with 1% and 2% of the body weight in concentrate. The animals were allotted to the treatments in a completely randomized experimental design, in a 3 × 3 factorial arrangement. The animals fed the ad libitum diet with more concentrate had greater cold carcass weight, cold carcass yield and longissimus muscle area/100 kg of cold carcass weight when compared with animals fed the diet with low concentrate allowance. There was no difference between the ad libitum diets for all of the carcass cuts yields, except for top sirloin. Crossbred animals had cold carcass yield larger than the Nellore animals. F1 Angus × Nellore animals had greater rib fat thickness and smaller longissimus muscle area/100 kg than the F1 Simmental × Nellore cattle. The yield of commercial carcass cuts was not influenced by genetic group, except for the shoulder clod yield, which was smaller in crossbred animals compared with Nellore animals. The longissimus muscle area, rib fat thickness and rump fat, taken by ultrasound were influenced by the body weight of the animals. Crossbred animals fed diets with higher concentrate level produce carcasses most suitable for the requirements of the market.

  2. Avaliação físico-química e microbiológica da carne soleada do Pantanal Physicochemical and microbiological evaluation of Pantanal's soleada beef

    Directory of Open Access Journals (Sweden)

    Larissa Lima Alves

    2010-09-01

    Full Text Available A carne soleada é um produto similar à carne de sol e originária exclusivamente do Pantanal. O objetivo foi avaliar variáveis físico-químicas e microbiológicas da carne soleada produzida em sistema modelo. Foram avaliados os efeitos da utilização dos recursos tecnológicos do congelamento e embalagem a vácuo sobre as variáveis microbiológicas. Prepararam-se amostras usando o corte comercial coxão duro (m. biceps femural e avaliadas as variáveis de pH, atividade de água, teor de cloretos e perdas de água por pressão, exsudação e cozimento. As contagens de S. aureus e E. coli foram realizadas na matéria-prima, carne soleada in natura e carne soleada congelada e embalada a vácuo. Os resultados mostram que a carne soleada precisa de adaptações para que se possa sugerir sua produção em escala. A atividade de água com valor médio de 0,97, pH em 5,72 e teor de cloretos em torno de 2,7% sugerem um produto com baixa vida de prateleira e que pode ser substrato para microrganismos patogênicos para o homem. O congelamento e a embalagem a vácuo não são recursos adequados para a conservação da carne soleada tradicional.Soleada beef is similar to sun-dried beef, and it is typical of Pantanal region. The aim of this study was to assess the physicochemical and microbiological variables of soleada beef produced in a model system. The effects of freezing technology resources and vacuum packing on the microbiological variables were assessed. Hence, biceps femoris samples were prepared, and the variables pH, water activity, chloride content, and water loss through pressure, sweating, and cooking were evaluated. The samples were separated into two aliquots; one was analyzed immediately and the other was vacuum packed and frozen. The S. aureus and E. coli count was done in the raw material, soleada beef in natura, and in frozen and vacuum packed soleada beef. Water activity with an average value of 0.97, pH of 5.72, and chloride

  3. Microbiologic quality water from

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    Adriano Luís Ferriani Junior

    2004-06-01

    Full Text Available The present work has as objective to evaluate the quality of the water of the Ribeirão dos Porcos river, at Espírito Santo do Pinhal-SP, Brazil, through microbiologycal anlyses for fecal and total coliform, fecal enterococci, pH, oxygen dissolved. Twenty four samples of water of 6 different points were collected, being made 4 collections of each point, in copies. The microbiologycal analyses, was accomplished by the method of the Most Probable Number (NMP using by multiple tubes technique. Determination of dissolved oxygen and pH values were made. The results of the microbiologycal analyses showed high levels of fecal and total coliforms (1,1x 105 to 2,4x 105/100 ml from point 1 to 6. In the point 1 (nascent, the fecal total coliforms and fecal enterococci, was inside of the acceptable limits. The results showed largest pollution indexes with fecal coliforms, of the point 2 to 6, mainly in the urban zone, where the river receives domestic and industrial effluents.

  4. ESCHERICHIA COLI O157 IN WITHE VEAL CALVES CARCASSES

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    V. D’Orio

    2013-02-01

    Full Text Available During 2006 one hundred and three white veal calves slaughtered at an abattoir in Isernia (Italy were examined for E. coli O157 intestinal carriage and carcass contamination, using Immuno-Magnetic Separation (IMS and multiplex PCR. Faecal material before slaughtering and carcass samples, using sponge-bag, were collected. E. coli O157 isolates were found in 10 (9,71% faecal and 9 (8,7% carcass samples. PCR analysis showed that all the strains from faecal and from carcass samples carried eaeA, hlyA and stx2 genes, while five strains stx1 gene. The results confirm that the slaughter practices can largely influence the rate of E. coli O157 carcasses contamination and suggest that white veal calves meat could be an important risk for human health.

  5. Haematology, Blood Chemistry and Carcass Characteristics of Growing Rabbits Fed Grasshopper Meal as a Substitute for Fish Meal

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    A. A. Njidda* and C. E. Isidahomen1

    2010-01-01

    Full Text Available An experiment was conducted to evaluate the effect of replacing fish meal with grasshopper meal on haematology, blood chemistry and carcass characteristics of growing rabbits. Forty rabbits of mixed breeds, aged 6-10 weeks, were randomly assigned to the dietary treatments in a complete randomized design with eight rabbits per treatment. The rabbits were fed with diets containing 0, 1.25, 2.50, 3.75 and 5% grasshopper meal in diets designated as T1 (control, T2, T3, T4 and T5, respectively. The experimental diets and clean drinking water were supplied ad libitum throughout the experimental period of nine weeks. At the end of the feeding trial, three rabbits per treatment were slaughtered for carcass evaluation, while blood samples were collected for analysis. The result of the experiment showed significant differences (P0.05 on haemoglobin and mean corpuscular haemoglobin concentration (MCHC. The results also revealed significant differences (P0.05 on serum albumin and total protein. The results of carcass characteristics showed significant differences among treatments (P<0.05 for slaughter weight, carcass weight, dressing percentage, skin pelt, tail, feet and abdominal fat. The slaughter weight and carcass weight were better in groups receiving 2.5% grass hopper meal (50% fish meal replacement. From the results, it can be concluded that inclusion of 2.50% grasshopper meal as a replacement for fish meal (50% replacement has no adverse effects on the haematological parameters, serum biochemistry and carcass characteristics of rabbits.

  6. [Microbiology--laboratory examinations for bacterias].

    Science.gov (United States)

    Hen, Renjun; Imafuku, Yuji; Yoshida, Hiroshi

    2002-11-01

    As it has been required to identify pathogenic microbes in shorter times, simple and rapid methods have been developed and used. Here, we summarized the present situation of rapid diagnostic testing in clinical microbiology in Japan, and also presented our results on PBP2' detection. The rapid test kits available in Japan for E. coli, Helicobacter pylori, Salmonella, Streptococcus and Staphylococcus aureus were described. Rapid examination methods are based mainly on immunologic reactions, which included slide agglutination using latex particle, immunochromatography and ELISA. Times required for the identification are 10 to 15 minutes. Moreover, rapid test kits employing PCR are also marketed. Further, we evaluated MRSA-LA "Seiken" which is a rapid detection kit for PBP2' produced by MRSA. The test was shown to be highly sensitive and specific. For the rapid identification of pathogenic microbes, simple and rapid test kits described here will be used more in clinical diagnosis.

  7. Microbiological Contamination of Cosmetic Creams in Tehran

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    Keshtvarz, M. (Msc

    2014-06-01

    Full Text Available Background and Objective: Transmission of pathogens by cosmetics is one of the major health complications. Direct contact with contaminated non-standard cosmetics can have irreparable side effects for the consumers. Thus, the evaluation of microbial contamination in cosmetic products is important. The aim of this study was to assess the microbiological contamination of one of frequently used cream. Material and Methods: In the present study, 135 samples of a special moisturizing cream were randomly selected from pharmacies in Tehran. The microbial contamination assessment, sampling and culturing method were based on the protocol (No.3978 of Iranian Institute of Standard and Industrial Research. Results: sixty-two (46% out of 135 samples were contaminated. The highest and lowest contaminations observed were Pseudomonas aeruginosa and Bacillus, respectively. Conclusion: Due to the high contamination rate of cosmetic creams, we recommend extremely monitoring and controlling these products by health centers. Keywords: Cosmetics, Microbial Contamination, Pseudomonas Aeruginosa

  8. Meat and carcass traits of the red-winged tinamou (Rhynchotus rufescens

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    FA de Queiroz

    2013-06-01

    Full Text Available Tinamou (Rhynchotus rufescens is a native bird of the Brazilian fauna and has great potential to become a domesticated species. From this perspective, the objective of this study was to evaluate the carcass and main parts yield and to assess meat quality of that bird. Two trials, one studying stocking density and the other litter materials, lasting 60 days each, were conducted. The studied traits were evaluated in two birds/replicate/treatment. Performance was evaluated by the least square method. The results showed that bird age at slaughter significantly influenced carcass and breast yields (p<0.05. Mean carcass, breast, thighs+drumstick and wing yields were 86.50±2.78%, 32.84±1.50%, 27.71±1.44%, and 13.21±0.94%, respectively. Shear force, pH, water holding capacity, lightness, redness and yellowness means and standard deviations were 1.92±0.98 kgf.cm-2, 6.07±0.12; 70±0.53%, 48.30±0.96, 1.96±1.01, and 1.61±1.0, respectively. Moisture, ash, crude protein, fat, and cholesterol contents were 72.35±0.16%, 1.12±0.06%, 25.53±0.50%, 0.17±0.01%, and 18.87±2.76 mg/10g, respectively. Results showed carcass and parts yields and the physical-chemical traits of tinamou meat are similar to chicken meat; however, tinamou meat is less acidic and has higher protein level combined with less calories and lower cholesterol levels.

  9. Carcass and carcass' cuts of F1 crossbred chickens of Pelung x Kampung given diets varied in protein content

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    Sofjan Iskandar

    1999-03-01

    Full Text Available Observation on carcass and carcass' cuts of F1 crossbred of Pelung x Kampung chickens influenced by dietary protein (15%, 17%, 19%, and 21% crude protein=CP was carried out at the Research Institute for Animal Production, Ciawi Bogor. There were 360-day-old chicks of the crossbred, allocated to 9 dietary-protein treatments. The treatments were P1 (21%-17% CP, which was the ration with 21% CP given to chicks aged of 0-6 weeks, then continued with 17% CP ration up to 12 week of age; P2 was 21%-15% CP; P3 was 19%-19% CP; P4 was 19%-17% CP; P5 was 19%-15% CP; P6 was 17%-17% CP; P7 was 17%- 15% CP; P8 was 15%-19% CP and P9 was 15%-15% CP. At 6 and 12 weeks of age each two out of 10 birds per cage were randomly picked for carcass and carcass portion analysis. Results showed that carcass and wings of 6 weeks of age birds were not significantly (P>0.05 influenced by dietary protein, whilst breast and thighs & drumsticks were significantly (P<0.05 lower on lower dietary protein. The weight of carcass and carcass parts were 256, 58, 71, 32, 44, 8.6, 9.7 and 2.09 g/bird, for carcass, breast, thighs and drumsticks, wings, oval; liver, gizzard and abdominal fat, respectively. At 12 weeks of age, weight of carcass and carcass cuts were not affected by dietary protein. The weight of carcass and carcass' cuts of 12 weeks of age were 803, 189, 251, 102, 123, 20, 25 and 21 g/bird, for carcass, breast, thighs and drumsticks, wings, oval, liver, gizzard and abdominal fat, respectively. The results indicated that ration with 15% CP given up to 12 weeks of age was recommended to be optimum without any reduction in carcass quality.

  10. Avaliação microbiológica de amostras de mel de trigoníneos (Apidae: Trigonini do Estado da Bahia Microbiological evaluation of trigonine bee (Apidae: Trigonini honey samples from the State of Bahia - Brazil

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    Bruno de Almeida Souza

    2009-12-01

    Full Text Available O mel é um produto que apresenta atividade antimicrobiana atribuída a fatores físicos e químicos. Mesmo assim, ainda é possível encontrar uma série de microrganismos presentes neste produto e que servem como indicadores de qualidade. O objetivo deste trabalho foi avaliar a qualidade microbiológica do mel produzido por espécies de abelhas sem ferrão (Trigonini do Estado da Bahia. Quatorze amostras de mel foram avaliadas quanto ao teor de umidade, atividade de água, contagem padrão de bolores e leveduras, e presença de microrganismos do grupo coliforme. Um total de 50,0% das amostras apresentou contagem padrão para bolores e leveduras acima do máximo permitido pela regulamentação brasileira para alimentos. Esta desclassificação de amostras assepticamente colhidas indica a necessidade de identificação desta microbiota e sua possível ocorrência natural no mel produzido por este grupo de abelhas. Nenhuma das amostras foi desclassificada em relação à contagem de microrganismos do grupo coliforme.Honey is a product that presents antimicrobial activity attributed to physical and chemical factors. Even so, it is still possible to find many microorganisms present in this product, which can be used as quality indicators. The objective of this work was to evaluate the microbiologic quality of the honey produced by stingless bee species from the State of Bahia, Brazil. Fourteen samples of honey were evaluated for the moisture content, water activity, standard counting of moulds and yeasts, and presence of microorganisms of coliform group. A total of 50.0% of the samples presented standard counting of moulds and yeasts above the maximum value permmited by the Brazilian food legislation. This disqualification of samples asseptically harvested indicates the need of identification of this microbiota and its possible natural occurrence in the honey produced by this group of bees. None of the samples was disqualified regarding the

  11. Evaluation of a multiplex real-time PCR method for detecting shiga toxin-producing Escherichia coli in beef and comparison to the U.S. Department of Agriculture Food Safety and Inspection Service Microbiology laboratory guidebook method.

    Science.gov (United States)

    Fratamico, Pina M; Wasilenko, Jamie L; Garman, Bradley; Demarco, Daniel R; Varkey, Stephen; Jensen, Mark; Rhoden, Kyle; Tice, George

    2014-02-01

    The "top-six" non-O157 Shiga toxin-producing Escherichia coli (STEC) serogroups (O26, O45, O103, O111, O121, and O145) most frequently associated with outbreaks and cases of foodborne illnesses have been declared as adulterants in beef by the U.S. Department of Agriculture Food Safety and Inspection Service (FSIS). Regulatory testing in beef began in June 2012. The purpose of this study was to evaluate the DuPont BAX System method for detecting these top six STEC strains and strains of E. coli O157:H7. For STEC, the BAX System real-time STEC suite was evaluated, including a screening assay for the stx and eae virulence genes and two panel assays to identify the target serogroups: panel 1 detects O26, O111, and O121, and panel 2 detects O45, O103, O145. For E. coli O157:H7, the BAX System real-time PCR assay for this specific serotype was used. Sensitivity of each assay for the PCR targets was ≥1.23 × 10(3) CFU/ml in pure culture. Each assay was 100% inclusive for the strains tested (20 to 50 per assay), and no cross-reactivity with closely related strains was observed in any of the assays. The performance of the BAX System methods was compared with that of the FSIS Microbiology Laboratory Guidebook (MLG) methods for detection of the top six STEC and E. coli O157:H7 strains in ground beef and beef trim. Generally, results of the BAX System method were similar to those of the MLG methods for detecting non-O157 STEC and E. coli O157:H7. Reducing or eliminating novobiocin in modified tryptic soy broth (mTSB) may improve the detection of STEC O111 strains; one beef trim sample inoculated with STEC O111 produced a negative result when enriched in mTSB with 8 mg/liter novobiocin but was positive when enriched in mTSB without novobiocin. The results of this study indicate the feasibility of deploying a panel of real-time PCR assay configurations for the detection and monitoring of the top six STEC and E. coli O157:H7 strains in beef. The approach could easily be adapted

  12. VARIATION OF THE ECONOMICAL EFFICIENCY OF PIG CARCASSES ACCORDING QUALITY CLASSES

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    I. PETROMAN

    2013-07-01

    Full Text Available The profitability of pork disjoint-packing activity depends on the carcass quality. Carcasses of superior types, respectively „E” and „U” generate higher profit on the kilogram of disjoint carcass, in comparison with the carcasses of medium type „R” and inferior types „O” and „P”.

  13. 9 CFR 311.8 - Cattle carcasses affected with anasarca or generalized edema.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Cattle carcasses affected with... ADULTERATED CARCASSES AND PARTS § 311.8 Cattle carcasses affected with anasarca or generalized edema. (a) Carcasses of cattle found on post-mortem inspection to be affected with anasarca in advanced stages...

  14. Relationship between teat morphology and microbiological quality of sheep milk

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    Sylwia Bielińska-Nowak

    2012-12-01

    Full Text Available The aim of the study was to assess the size of the udder and morphological changes in ewe’s teats, as well as relationship of these features with microbiological quality of milk. The examinations of morphological structures of ewe’s teats were performed with 10 MHz ultrasound linear probe. The experiment was conducted on 40 dairy sheep, in three consecutive calendar years. To clarify the correlation between total bacteria count, teat morphology and size of the udder, the results of determination of the microbiological quality of milk were grouped in four ranges: total bacteria count < 50 000, 50 000-100 000, 100 000-200 000 and > 200 000 in 1 cm3 of milk. The width of the udders increased with increasing concentration of total bacteria count (p < 0.001. The widest udders were observed in sheep with the highest total bacteria count concentration – up to 200 000 of bacteria in 1 cm3 of milk: 122.7 mm. With increasing concentration of total bacteria count in milk, decreased udder height (p < 0.001. The smallest height of udder was found in ewes producing milk of the lowest microbiological quality (p < 0.001. The experiment showed no statistically significant correlations between teat’s length, width, morphological structures and total bacteria count (p > 0.05. Therefore, the application of these features in a rapid evaluation of the microbiological quality of sheep milk is unusable.  

  15. Effect of source of dietary fat on pig performance, carcass characteristics and carcass fat content, distribution and fatty acid composition.

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    Realini, C E; Duran-Montgé, P; Lizardo, R; Gispert, M; Oliver, M A; Esteve-Garcia, E

    2010-08-01

    Seventy gilts were used to compare the effect of including 10% tallow (T), high-oleic sunflower oil (HOSF), sunflower oil (SFO), linseed oil (LO), a fat blend (FB), or an oil blend (OB) in finishing diets vs. feeding a semi-synthetic diet with no added fat (NF) on pig performance, carcass traits and carcass fatty acid (FA) composition. Carcasses from SFO-fed gilts had greater fat and lower lean compositions than carcasses from T-fed gilts. Gilts fed NF had greater loin fat than FB-fed gilts, and greater flare fat, loin intermuscular fat and fat:lean than T-fed gilts. Bellies from NF-fed gilts had lower lean and higher intermuscular fat and fat:lean than other diets except HOSF. Fat source had minor effects on animal performance, carcass characteristics and carcass fat content and distribution, whereas feeding NF resulted in carcasses and major cuts with higher fat content. Diets rich in polyunsaturated FA (PUFA) did not reduce fat deposition in separable fat depots with respect to monounsaturated FA (MUFA) and saturated FA (SFA). Carcasses from gilts fed NF had a high degree of saturation (40.6% SFA) followed by carcasses of T- and FB-fed gilts. Feeding HOSF, SFO and LO enriched diets elevated the percentages of MUFA (56.7%), n-6 (30.0%) and n-3 (16.6%) PUFA, respectively, whereas carcasses from gilts fed OB had greater percentages of n-3 FA (14.8% n-3, 0.9% EPA, 1.0% DPA, 3.1% DHA) than gilts fed FB (6.72% n-3, 0.1% EPA, 0.4% DPA, 0.1% DHA).

  16. Babassu mesocarp bran levels associated with whole or ground corn grains in the finishing of young bulls: carcass and meat characteristics

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    Aline Evangelista Machado Santana

    2014-11-01

    Full Text Available The objective of this study was to evaluate the effect of grinding corn and inclusion of babassu mesocarp bran (BMB in the diet of crossbred dairy young bulls finished in feedlot on their carcass and meat characteristics. Twenty-four young bulls were fed four experimental diets containing two BMB inclusion levels (0 and 41.24% and corn in two forms of processing (ground and whole grains for 98 days, and then slaughtered in a commercial packing plant. Among the quantitative carcass characteristics, there was interaction only in fat trimmings/whole hot carcass weight and cold carcass yield. The whole hot carcass and hot carcass weights showed reduced means with the use of whole corn. The other quantitative variables were not separately affected by BMB inclusion levels or by the form corn was processed. There was interaction between the factors for pistol cut weight, edible portion and weight of the cuts of the pistol cut, in which the use of whole corn and BMB promoted reduction in the mean values. The use of the whole corn grain reduced the participation of flank cut and elevated the participation of the bone fraction in the carcass. Inclusion of 41.24% of BMB did not change the qualitative meat characteristics, but the use of corn grain resulted in darker meat. The use of whole corn resulted in alteration in the weight of cuts like eye of rump and striploin, which have a high commercial value. There was interaction between factors for the percentages of moisture and minerals of the meat, whereas the crude protein content was affected by the two factors. The use of diets with whole corn or with babassu mesocarp bran may modify the carcass and meat characteristics of finishing crossbred young bulls.

  17. The crossbreeding of improved Jezersko-Solcˇava sheep with Charollais to improve carcass traits

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    Dragomir Kompan

    2010-01-01

    Full Text Available Thirteen improved Jezersko-Solcˇava lambs (JSR and 16 crossbreds with Charollais (JSRxCH were used to evaluate the effect of genotype and sex on carcass traits. Lambs were weaned at around 60 days of age. They were fed with commercial concentrate and hay ad libitum and slaughtered at 30 kg of average live weight at 103 days of age. Crossbred lambs had at the same live weight at slaughter better conformation (9.19 on 15 points scale than JSR lambs (7.58. Carcasses of crossbred lambs had lower proportion of fat in hind leg and lower proportion of kidney fat. Female lambs had better dressing proportion (46.25% than male lambs (42.72%. Females had also higher scores for both internal and subcutaneous fatness and higher percentage of kidney fat. They had higher proportion of fat and lower proportion of bone in hind leg.

  18. Lack of association of bovine MHC class I alleles with carcass and reproductive traits.

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    Arriëns, M A; Hofer, A; Obexer-Ruff, G; Lazary, S

    1996-12-01

    The present study was carried out to examine whether a relationship between bovine major histocompatibility complex (BoLA) class I alleles and carcass traits or reproductive performance exists in Braunvieh and Fleckvieh AI (artificial insemination) bulls. The influence of BoLA class I (BoLA-A) alleles on deregressed breeding values for net growth rate, carcass index and thigh volume was assessed in Braunvieh crosses and Fleckvieh bulls with a gene substitution model. The reproductive traits: non-return rate and interval between first and last insemination of daughters (female fertility), as well as non-return rate of inseminated cows (male fertility), were only investigated in Fleckvieh animals. No influence of the BoLA-A region on the traits evaluated could be demonstrated. An improper, i.e. less restrictive analysis would have led to spurious results.

  19. Carcass characteristics, the calpain proteinase system, and aged tenderness of Angus and Brahman crossbred steers.

    Science.gov (United States)

    Pringle, T D; Williams, S E; Lamb, B S; Johnson, D D; West, R L

    1997-11-01

    We used 69 steers of varying percentage Brahman (B) breeding (0% B, n = 11; 25% B, n = 13; 37% B, n = 10; 50% B, n = 12; 75% B, n = 12; 100% B, n = 11) to study the relationship between carcass traits, the calpain proteinase system, and aged meat tenderness in intermediate B crosses. Calpains and calpastatin activities were determined on fresh longissimus muscle samples using anion-exchange chromatography. The USDA yield and quality grade data (24 h) were collected for each carcass. Longissimus steaks were removed and aged for 5 or 14 d for determination of shear force and 5 d for sensory panel evaluation. Even though some yield grade factors were affected by the percentage of B breeding, USDA yield grades did not differ (P > .15) between breed types. Marbling score and USDA quality grade decreased linearly (P Brahman crosses. PMID:9374310

  20. Carcass characteristics, the calpain proteinase system, and aged tenderness of Angus and Brahman crossbred steers.

    Science.gov (United States)

    Pringle, T D; Williams, S E; Lamb, B S; Johnson, D D; West, R L

    1997-11-01

    We used 69 steers of varying percentage Brahman (B) breeding (0% B, n = 11; 25% B, n = 13; 37% B, n = 10; 50% B, n = 12; 75% B, n = 12; 100% B, n = 11) to study the relationship between carcass traits, the calpain proteinase system, and aged meat tenderness in intermediate B crosses. Calpains and calpastatin activities were determined on fresh longissimus muscle samples using anion-exchange chromatography. The USDA yield and quality grade data (24 h) were collected for each carcass. Longissimus steaks were removed and aged for 5 or 14 d for determination of shear force and 5 d for sensory panel evaluation. Even though some yield grade factors were affected by the percentage of B breeding, USDA yield grades did not differ (P > .15) between breed types. Marbling score and USDA quality grade decreased linearly (P Brahman crosses.

  1. Tenderization potential of Hanwoo beef muscles from carcasses with differed genders and loin intramuscular fat content levels during post mortem ageing.

    Science.gov (United States)

    Park, Beom Young; Seong, Pil Nam; Ba, Hoa Van; Park, Kyoung Mi; Cho, Soo Hyun; Moon, Sung Sil; Kang, Geun Ho

    2015-06-01

    Carcasses from Hanwoo steers (n = 15) and cows (n = 15) were classified into three groups: group 1 (G1), the carcasses had 10% to muscles; group 2 (G2), the carcasses had 13% to muscles; and group 3(G3), the carcasses had 17% to muscles. These were used to evaluate the effects of gender and carcass group on quality traits and Warner-Bratzler shear force (WBSF) of Psoas major (PM), Longissimus thoracis (LT), Longissimus lumborum (LL), Longus colli (LC), Supraspinatus (SS), Latissimus dorsi (LAD), Semimembranosus (SM), Quadriceps femoris (QF), Biceps femoris (BF) and Semitendinosus (ST) muscles. Our results showed that pH values of LT, LL, LC, BF and QF muscles were lower in steers than in cows (P muscles of steers (P muscles in G1, and QF muscle in G3; however, with additional ageing, the gender effect was observed for most of the muscles. Most muscles showed ageing responses; however, the rates of ageing response significantly varied depending on gender and carcass groups. The muscles of G1 and G2 had generally higher tenderization potentials than those of G3. Furthermore, most muscles in G3 had generally lower WBSF values than in G1 and G2. These results clearly indicate that ageing has a significant effect on quality and WBSF of beef muscles, and the classification by loin IMF level may be useful for prediction of the tenderness of other muscles.

  2. Performance, body measurements, carcass and cut yields, and meat quality in lambs fed residues from processing agroindustry of fruits

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    Darcilene Maria de Figueiredo

    2015-02-01

    Full Text Available This research was conducted with the objective to evaluate the use of residue dry matter (DM from pineapple (Ananas comosus L., banana (Musa sp., mango (Mangifera indica and passion fruit (Passiflora spp. in feeding of the feedlot on productive performance, carcass yield and qualitative and quantitative characteristics of meat. Twenty-five crossbred lamps with Santa Inês breed and mixed breed were used. The treatments consisted of the replacement of 75% of sorghum silage by respective residue DM, whereas in the control treatment forage had only sorghum silage the diets had a houghageto- concentrate ratio of 40:60 interns of DM being isonitrogenous and isoenergetics. The animals were slaughtered at 32 kg liveweight. Before slaughter were obtained biometric measurements, after the same, was performed the hot carcasses weight and morphometric measurements. After 24 hours in a cold chamber at 4 ° C, was determined the cold carcass weight and yield calculation. The left half carcass was divided into five sections: neck, shoulder, shank, rib and loin, by performing the calculation of income cuts. Analyses meat quality such as pH, color (L, a, b, chroma and Ho, by cooking weight loss, water retention capacity and shear strength were carried out in the Longissimus dorsi sample. The completely randomized design was adapted. The data were interpreted using analysis of variance with the test a Tukey 5% probability. There was no effect of diet (P> 0.05 according to the parameters: growth performance, body measurements, and meat quality of lambs. There was also no effect of the diets (P> 0.05 on the loin eye area assuming that carcasses remained similar muscularity important fact to market acceptance standard. It is concluded that replacing up to 75% of sorghum silage by residues fruit (pineapple, banana, mango and passion fruit in lambs feeding becomes feasible not to change the productive performance, body measurements, yields carcass and cuts and meat

  3. Partial Replacement of Ground Corn with Glycerol in Beef Cattle Diets: Intake, Digestibility, Performance, and Carcass Characteristics.

    Science.gov (United States)

    Del Bianco Benedeti, Pedro; Paulino, Pedro Veiga Rodrigues; Marcondes, Marcos Inácio; Maciel, Ivan França Smith; da Silva, Matheus Custódio; Faciola, Antonio Pinheiro

    2016-01-01

    The objective of this study was to evaluate the effects of replacing dry ground corn with crude glycerol on intake, apparent digestibility, performance, and carcass characteristics of finishing beef bulls. A completely randomized block design experiment with 25 d for adaptation and 100 d for data collection was conducted, in which 3,640 Nellore bulls (367 ± 36.8 kg; 18 ± 3 mo) were blocked by body weight and assigned to 20 pens. Bulls were randomly assigned to one of four treatments: 0, 5, 10, and 15% (dry matter basis) of crude glycerol in the diet. Initially, 20 bulls were slaughtered to serve as a reference to estimate initial empty body weight, which allowed for carcass gain calculation. Bulls were weighed at the beginning, at two-thirds, and at the end of the experiment for performance calculations. Carcass measurements were obtained by ultrasound. Fecal output was estimated using indigestible neutral detergent fiber as an internal marker. Data were analyzed using the mixed procedures in SAS 9.2 (SAS Institute Inc., Cary, NC). Intake of dry matter, organic matter, and neutral detergent fiber decreased linearly (P 0.05) intakes of crude protein, non-fiber carbohydrates, and total digestible nutrients. Digestibility of dry matter, organic matter, neutral detergent fiber, and total digestible nutrients increased quadratically (P change the intake of digestible dry matter, average daily gain, final body weight, carcass gain, carcass dressing, gain-to-feed ratio, Longissimus thoracis muscle area, and back and rump fat thicknesses (P > 0.05). These results suggest that crude glycerol may be included in finishing beef diets at levels up to 15% without impairing performance and carcass characteristics. PMID:26820725

  4. Microbiology Learning and Education Online.

    Science.gov (United States)

    Guarner, Jeannette; Niño, Silvia M

    2016-05-01

    The ubiquity of devices that connect to the Internet has exploded, allowing for easy dissemination of information. Many teachers from kindergarten to universities use the information obtained online or post material they want their students to access. Online media readily places articles, books, videos, and games at our fingertips. The public in general also gathers health information from the Internet. The following review will explore what has been published regarding microbiology education and learning online and the use of electronic media by microbiologists for scientific purposes. PMID:26935727

  5. Microbiology Learning and Education Online.

    Science.gov (United States)

    Guarner, Jeannette; Niño, Silvia M

    2016-05-01

    The ubiquity of devices that connect to the Internet has exploded, allowing for easy dissemination of information. Many teachers from kindergarten to universities use the information obtained online or post material they want their students to access. Online media readily places articles, books, videos, and games at our fingertips. The public in general also gathers health information from the Internet. The following review will explore what has been published regarding microbiology education and learning online and the use of electronic media by microbiologists for scientific purposes.

  6. Carcass composition of market weight pigs subjected to heat stress in utero and during finishing.

    Science.gov (United States)

    Cruzen, S M; Boddicker, R L; Graves, K L; Johnson, T P; Arkfeld, E K; Baumgard, L H; Ross, J W; Safranski, T J; Lucy, M C; Lonergan, S M

    2015-05-01

    Objectives were to investigate the effects of prolonged gestational and/or postnatal heat stress on performance and carcass composition of market weight pigs. Pregnant gilts were exposed to gestational heat stress (GHS, 28°C to 34°C, diurnal) or thermal neutral (18°C to 22°C, diurnal) conditions during the entire gestation or during the first or second half of gestation. At 14 wk of age (58 ± 5 kg), barrows were housed in heat stress (32°C, HS) or thermal neutral (21°C, TN) conditions. Feed intake and BW were recorded weekly, and body temperature parameters were monitored twice weekly until slaughter (109 ± 5 kg). Organs were removed and weighed, and loin eye area (LEA) and back fat thickness (BF) were measured after carcass chilling. Carcass sides were separated into lean, separable fat, bone, and skin components and were weighed. Moisture, lipid, and protein content were determined in the LM at the 10th rib. Data were analyzed using a split plot with random effect of dam nested within gestational treatment. Carcass measurements included HCW as a covariate to control for weight. Planned orthogonal contrast statements were used to evaluate the overall effect of GHS in the first half, second half, or any part of gestation. Gestational heat stress did not alter postnatal performance or most body temperature parameters (P > 0.10). However, ADFI in the finishing period was increased (P pigs receiving GHS in the first half of gestation. Gestational heat stress during the first half of gestation decreased head weight as a percent of BW (P = 0.02), whereas GHS in the second half of gestation decreased bone weight as a percent of BW (P = 0.02). Heat stress reduced ADG, BW, and HCW (P pigs on both a weight and percentage basis (P pigs. In summary, GHS may affect head and bone development, subsequently affecting carcass composition. Chronic HS during finishing results in longer times to reach market weight and a leaner carcass once market weight is achieved.

  7. Impacts of temperament on Nellore cattle: physiological responses, feedlot performance, and carcass characteristics.

    Science.gov (United States)

    Francisco, C L; Resende, F D; Benatti, J M B; Castilhos, A M; Cooke, R F; Jorge, A M

    2015-11-01

    Forty-four feedlot-finished Nellore cattle were used to evaluate the impacts of temperament on performance, meat and carcass traits, and serum concentrations of hormones, proteins, enzymes, and immunoglobulins. Individual temperament was assessed at feedlot entry (d 0), 67 d, and 109 d, utilizing chute score (CS; 5-point scale) and exit velocity (EV). Temperament scores were calculated averaging CS and EV scores, and cattle were subsequently classified according to their temperament (an average of ≤3 = adequate temperament [ADQ], or an average of >3 = excitable temperament [EXC]). At the end of the experiment (d 109), all 44 animals were slaughtered, and 16 were randomly selected for final empty body weight (EBW) estimation. Blood samples were collected at 0, 67, and 109 d and analyzed for serum variables (cortisol, insulin, haptoglobin, total protein, lactate, creatinine kinase [CK], lactate dehydrogenase [LDH], and IgA). The incidence of carcass bruises was verified immediately after the hide was removed. Carcass pH was obtained at 0 and 24 h postmortem. Samples of the LM were collected for meat quality analyses. Cattle classified as ADQ had greater final BW ( = 0.03), final EBW ( = 0.02), metabolic weight ( = 0.03), ADG ( = 0.02), feed efficiency ( = 0.03), HCW ( = 0.02), cold carcass weight ( = 0.02), and LM area ( Cattle classified as ADQ tended to have a lower percentage of cooler shrink ( = 0.06) compared to that of EXC cattle. No temperament effects were detected for initial BW ( = 0.70), DMI ( = 0.14), cold dressing percentage ( = 0.98), or backfat thickness ( = 0.29). Cattle classified as ADQ had greater marbling ( = 0.02) and meat fat content ( = 0.05) compared with that of EXC cattle. No temperament effects ( > 0.05) were detected for unsaturated fatty acid (UFA), SFA, MUFA, PUFA, and n-6:n-3 ratio. For blood parameters, EXC cattle had greater values of cortisol ( = 0.04) and haptoglobin ( = 0.05) and tended ( = 0.06) to have reduced serum insulin

  8. Performance and carcass characteristics of free-range broiler chickens fed diets containing alternative feedstuffs

    Directory of Open Access Journals (Sweden)

    PB Faria

    2011-09-01

    Full Text Available The present study was carried out to evaluate the effects of alternative feedstuffs as partial substitutes of corn and soybean in free-range broiler diets on performance, carcass yield and technical-economic viability. A total of 400 Pescoço Pelado broilers were distributed in a completely randomized experimental design (CRD, with four treatments (treatment 1:Control; treatment 2: 10% rice bran inclusion; treatment 3: 10% ground cassava leaves; and treatment 4: 10% ground lead tree hay with four replicates per treatment. Each replicate consisted of a group of 25 birds per paddock, separated per sex. Initial weight (IW, final weight (FW, body weight (BW, daily weight gain (DWG, feed intake (FI and feed conversion ratio (FCR were evaluated. Carcass, cuts (breast, thigh, drumstick, back, neck, leg and wings, abdominal fat and giblets (gizzard, heart and liver yields were determined. The technical-economic viability of each treatment was assessed by determining the cost of feed per kg body weight, economic efficiency index and cost. The highest final weights were obtained with the use of rice bran. Rice bran and cassava leaves promoted higher carcass yield, as well as lower back and abdominal fat yields. The use of cassava leaves showed better economic efficiency among the treatments with alternative feedstuffs. The use of alternative feedstuffs at 10% inclusion in substitution of corn and soybean meal did not not result in major changes in performance and carcass parameters, and economic efficiency, and therefore, their use is recommended when the availability or the price of key ingredients, such as soybean meal and corn, increase.

  9. Validation of a same-day real-time PCR method for screening of meat and carcass swabs for Salmonella

    Directory of Open Access Journals (Sweden)

    Hansen Flemming

    2009-05-01

    Full Text Available Abstract Background One of the major sources of human Salmonella infections is meat. Therefore, efficient and rapid monitoring of Salmonella in the meat production chain is necessary. Validation of alternative methods is needed to prove that the performance is equal to established methods. Very few of the published PCR methods for Salmonella have been validated in collaborative studies. This study describes a validation including comparative and collaborative trials, based on the recommendations from the Nordic organization for validation of alternative microbiological methods (NordVal of a same-day, non-commercial real-time PCR method for detection of Salmonella in meat and carcass swabs. Results The comparative trial was performed against a reference method (NMKL-71:5, 1999 using artificially and naturally contaminated samples (60 minced veal and pork meat samples, 60 poultry neck-skins, and 120 pig carcass swabs. The relative accuracy was 99%, relative detection level 100%, relative sensitivity 103% and relative specificity 100%. The collaborative trial included six laboratories testing minced meat, poultry neck-skins, and carcass swabs as un-inoculated samples and samples artificially contaminated with 1–10 CFU/25 g, and 10–100 CFU/25 g. Valid results were obtained from five of the laboratories and used for the statistical analysis. Apart from one of the non-inoculated samples being false positive with PCR for one of the laboratories, no false positive or false negative results were reported. Partly based on results obtained in this study, the method has obtained NordVal approval for analysis of Salmonella in meat and carcass swabs. The PCR method was transferred to a production laboratory and the performance was compared with the BAX Salmonella test on 39 pork samples artificially contaminated with Salmonella. There was no significant difference in the results obtained by the two methods. Conclusion The real-time PCR method for

  10. From the Field: Carbofuran detected on weathered raptor carcass feet

    Science.gov (United States)

    Vyas, N.B.; Spann, J.W.; Hulse, C.S.; Bauer, W.; Olson, S.

    2005-01-01

    The cause of death for raptors poisoned at illegal carbofuran-Iaced predator baits is often not confirmed because the carcass matrices that are conventionally analyzed are not available due to decomposition and scavenging. However, many such carcasses retain intact feet that may have come into contact with carbofuran. Eastern screech owls (Otus asio) were exposed to carbofuran via simulated predator baits. Detection of carbofuran from owl feet weathered for 28 days demonstrated the temporal reliability of using feet during a forensic investigation. Raptor carcasses previously not submitted for residue analysis because of a lack of the conventional matrices may now be salvaged for their feet.

  11. The impact of bone development on final carcass weight

    DEFF Research Database (Denmark)

    Tatara, M.R.; Tygesen, Malin Plumhoff; Sawa-Wojtanowicz, B.;

    2006-01-01

    Proper development and function of the skeleton is crucial for the optimal growth of an organism, with rapid growth rates often resulting in skeletal disorders in farm animals. Yet, despite clear benefits for breed selection and animal welfare (HARRISON et al., 2004), the impact of bone development...... was removed and its parameters correlated with carcass weight. Results suggest that both femur length and femur weight act as good predictors of final carcass weight in lambs. However, no effect of paternal genetics, on the femur to carcass correlations, was noted....

  12. Polymorphisms in positional candidate genes on BTA14 and BTA26 affect carcass quality in beef cattle.

    Science.gov (United States)

    Marques, E; Nkrumah, J D; Sherman, E L; Moore, S S

    2009-08-01

    Several studies have reported the presence of carcass quality QTL on BTA14 and BTA26, with no specific genes being conclusively linked as their cause. The aim of this study was to identify polymorphisms in genes known to affect lipid metabolism in other species and to assess their association with carcass quality traits. Two genes located on BTA14, 2,4 dienoyl CoA reductase 1 (DECR1) and core binding factor, runt domain, alpha subunit 2, translocated to 1 gene (CBFA2T1), have been previously evaluated in other species and found to contain polymorphisms influencing lipid metabolism. A gene on BTA26, fibroblast growth factor 8 (FGF8), has in recent studies been linked to several QTL affecting obesity in mice, indicating its potential for regulating adiposity in other species. Sequencing analysis identified 9 polymorphisms in DECR1, 4 in CBFA2T1, and 4 in FGF8. Multiple sequence alignment of DECR1 among cattle, humans, and mice showed that 4 of these mutations lie in conserved regions across these species. Using 464 Angus, Charolais, and crossbred animals produced associations with ultrasound marbling score (CBFA2T1, P = 0.019), ultrasound backfat (DECR1, P = 0.012), carcass backfat (FGF8, P = 0.004), and lean meat yield (FGF8, P = 0.005). Quantitative trait loci analysis including a set of previously genotyped markers on BTA14, and 1 DECR1 polymorphism resulted in several significant QTL peaks: ultrasound backfat (UBF) at 91 cM, lean meat yield at 86 cM, carcass gradefat at 15 cM, and yield grade at 87 cM, all at the P yield at 2 cM and for yield grade at 25 cM, both at P < 0.01, and for carcass backfat at 25 cM (P < 0.05). Removal of FGF8 SNP in further analysis resulted in the disappearance of the carcass backfat QTL. These results suggest that polymorphisms discovered in DECR1, CBFA2T1, and FGF8 may play a role in the lipid metabolism pathway affecting carcass quality traits in beef cattle. However, further studies are needed to confirm that these polymorphisms

  13. Manual of Environmental Microbiology - Literature Review.

    Science.gov (United States)

    The field of environmental microbiology has made tremendous strides since the original microscopic observations of Antony van Leeuwenhock in 1677. The Manual of Environmental Microbiology, 3rd edition embraces these technological advances and is perhaps the most comprehensive and informative book s...

  14. Modeling the Biological Diversity of Pig Carcasses

    DEFF Research Database (Denmark)

    Erbou, Søren Gylling Hemmingsen

    extracting and modeling meaningful information from the vast amount of information available from non-invasive imaging data. The lean meat percentage (LMP) is a common standard for measuring the quality of pig carcasses. Measuring the LMP using CT and using this as a reference for calibration of online...... equipment is investigated, without the need for a calibration against a less accurate manual dissection. The rest of the contributions regard the construction and use of point distribution models (PDM). PDM’s are able to capture the shape variation of a population of shapes, in this case a 3D surface of a...... specific bone structure in the ham. These models can assist developers of robotic tools by enabling population based testing before actual construction of the tools. Sparse models are compared to the standard PCA based model and a method for fitting PDM’s to sparse data is proposed. The former provides...

  15. Ultrasonography as a predicting tool for carcass traits of young bulls

    Directory of Open Access Journals (Sweden)

    Suguisawa Liliane

    2003-01-01

    Full Text Available Considerable resources have been allocated to support research in the development of non-invasive and non-destructive techniques for carcass composition and quality evaluation. Ultrasonography is a reliable and relatively low-cost technique that can be used. In the present study, real-time ultrasonography was used to predict ribeye area (REA and subcutaneous fat thickness (FT in live animals as compared to carcass measurements. Animals used were 115 yearling bull calves (initial body weight, 329 kg, kept under feedlot conditions, of four genetic groups (30, ½ Angus x Nellore; 30, ½ Canchim x Nellore; 30, ½ Simmental x Nellore, and 25 Nellore, and two finishing frame sizes (small and large. Four ultrasonographic measurements were taken every 28 days until slaughter. Predictive precision of ultrasonographic measurements increased as animals approached slaughter, reaching maximum values at the last measurement (R²=0.68 and 0.82 for REA and FT, respectively. FT carcass measurements was influenced by genetic group and live measurements (P < 0.05. Frame size did not influence REA and FT, probably due to small, but distinctive differences among genetic groups.

  16. Meat and carcass characteristics of free-living capybaras (Hydrochoerus hydrochaeris

    Directory of Open Access Journals (Sweden)

    Gisele A. Felix

    2014-06-01

    Full Text Available This study aimed to evaluate carcass and meat characteristics of free-living capybaras (Hydrochoerus hydrochaeris from agricultural areas of Grande Dourados, MS, Brazil. Carcass weight, yield and measures, as well as of commercial cuts weight of five animals (2 males and 3 females, body weight between 63.4-100 kg were determined. The following parameters: moisture, protein, fat, ash, Ca, P and Fe, water holding capacity, cooking losses, shear force and color Instrumental color in CIE-Lab coordinates were determined to meat cuts (Loin, ribs, L. dorsi, chuck/brisket, ham and bottom sirloin. The dressing percentage average value was 62.47% of body weight and cold carcass yield was 57.89% of body weight, with no difference between sexes. The mean percentage of cold loss was 4.10%. Cuts yielding (ribs 24.98% and ham 27.29% were not statistically different (P>0.05. There was no significantly (P> 0.05 difference between the moisture, protein and minerals content between the different cuts, but lipids content was significantly (P< 0.05 lower in loin. The results shown that free-living capybara have characteristics appropriate for commercialization, thus it is possible to development projects for the sustainable management of rural populations.

  17. The Effect of Live Weight at Slaughter and Sex on Lambs Carcass Traits and Meat Characteristics

    Directory of Open Access Journals (Sweden)

    Silvester Žgur

    2003-09-01

    Full Text Available Twenty-eight (12 male and 16 female of improved Jezersko-solèava lambs with Romanov (JSR were used to evaluate the effect of live weight and sex on carcass traits and meat quality. Lambs were weaned at around 60 days of age. They were fed with commercial concentrate and hay ad libitum and slaughtered at 29 kg or 43 kg of average live weight at 105 or 126 days of age. Daily gain from birth to slaughter was higher than 300 g/day and was very similar for both groups. There were no differences between light and heavy lambs in carcass conformation and dressing percentage. Heavy lambs were longer, wider and fatter. The difference in lungs, head and pelt percentage were statistically significant. With increased live weight at slaughter the percentage of neck, back and rib with flank increased and chuck, shoulder and hindleg decreased. Muscle percentage in hindleg increased and bone percentage decreased as live weight increased. Considering meat quality, heavier lambs had lower lightness and higher redness. Lighter lambs had higher pH45 value. Differences between sexes were statistically significant for dressing percentage and carcass fatness with higher values for females. Males had higher percentage of liver and head, higher proportion of neck, chuck and shoulder and lower proportion of back and loin. Males tended to have higher values for muscle and bone proportion, and lower proportion of fat in hindleg. Males had lighter meat. Sex had no effect on pH values.

  18. The Effect of Live Weight at Slaughter and Sex on Lambs Carcass Traits and Meat Characteristics

    Directory of Open Access Journals (Sweden)

    Silvester Žgur

    2003-09-01

    Full Text Available Twenty-eight (12 male and 16 female of improved Jezersko-solèava lambs with Romanov (JSR were used to evaluate the effect of live weight and sex on carcass traits and meat quality. Lambs were weaned at around 60 days of age. They were fed with commercial concentrate and hay ad libitum and slaughtered at 29 kg or 43 kg of average live weight at 105 or 126 days of age. Daily gain from birth to slaughter was higher than 300 g/day and was very similar for both groups. There were no differences between light and heavy lambs in carcass conformation and dressing percentage. Heavy lambs were longer, wider and fatter. The difference in lungs, head and pelt percentage were statistically significant. With increased live weight at slaughter the percentage of neck, back and rib with flank increased and chuck, shoulder and hindleg decreased. Muscle percentage in hindleg increased and bone percentage decreased as live weight increased. Considering meat quality, heavier lambs had lower lightness and higher redness. Lighter lambs had higher pH45 value. Differences between sexes were statistically significant for dressing percentage and carcass fatness with higher values for females. Maleshad higher percentage of liver and head, higher proportion of neck, chuck and shoulder and lower proportion of back and loin. Males tended to have higher values for muscle and bone proportion, and lower proportion of fat in hindleg. Males had lighter meat. Sex had no effect on pH values.

  19. Genetic relationships and expected responses for genetic improvement of carcass traits of Berkshire pigs

    Directory of Open Access Journals (Sweden)

    Masamitsu Tomiyama

    2011-10-01

    Full Text Available The Berkshire pig (Sus domestica L. breed has thin muscle fibers and excellent water-holding capacity. The Berkshire meat makes it widely accepted in the Japanese premium pork market. This study evaluates the accuracy of improving carcass quality with the use of live animal records of Berkshire pigs. Traits analyzed in live animals were: body weight at 60 days of age (W60, age at finish (AGF, daily weight gain from birth to finish (DG, back fat thickness at finish (BFTF, and loin eye area at finish (LEAF, and in carcasses were: carcass weight, loin eye area (LEA, and subcutaneous fat thickness (SCF at some points, using the records of 4,773 purebred Berkshire pigs. Variance components for the traits were estimated according to the animal model by the Restricted Maximum Likelihood (REML procedure using the VCE6 program (Neumaier and Groeneveld, 1998. Correlated responses were also calculated. Genetic correlations of back fat thickness (BFT in live animals with SCF in slaughtered animals were strong, whereas that of LEA between live and slaughtered animals was low. The expected gains by actual selection including W60 and BFTF as selection criterion were superior to other selections. Therefore, selection of live animals at an early stage of growth would be conducive to the production of high quality meat.

  20. Effects of dietary lysine levels on carcass performance and biochemical characteristics of Chinese local broilers

    Directory of Open Access Journals (Sweden)

    Yuncong Yuan

    2015-08-01

    Full Text Available Lysine is typically the second-limiting amino acid in poultry diets. The objective of this study was to evaluate the effects of dietary lysine concentration on carcass and meat quality traits, and serum parameters in two lines – SD02 and SD03 – which originated from a Chinese local breed, the Erlang Mountainous chicken. Live body weight, carcass traits, meat quality traits (myofibre diameter and density, and serum metabolic markers were measured in high and low dietary lysine groups (HL and LL, respectively at the end of the starter (1-28 days, grower (29-49 days and finisher (50-70 days periods. The results showed that mortality, live weight (LW, myofibre diameter of leg muscle (LFDM and serum cholesterol (CHO were greater in HL than LL (P<0.05. The chickens from HL had reduced subcutaneous fat thickness and heart weight than LL (P<0.05. The chickens from line SD02 had greater leg muscle weight, myofibre diameter in breast, and LFDM than line SD03 (P<0.05. The chickens from line SD02 had more serum urea nitrogen and less total proteins than line SD03 (P<0.05. In conclusion, high lysine diets improved slaughter performance and muscle fibre diameter, and SD02 chickens had greater carcass yield and superior meat quality compared with chickens from line SD03.

  1. Effect of dietary energy and protein content on growth and carcass traits of Pekin ducks.

    Science.gov (United States)

    Zeng, Q F; Cherry, P; Doster, A; Murdoch, R; Adeola, O; Applegate, T J

    2015-03-01

    A study was conducted to determine the influence of dietary energy and protein concentrations on growth performance and carcass traits of Pekin ducks from 15 to 35 d of age. In experiment 1, 14-d-old ducks were randomly assigned to 3 dietary metabolizable energy (11.8, 12.8, and 13.8 MJ/kg) and 3 crude protein concentrations (15, 17, and 19%) in a 3×3 factorial arrangement (6 replicate pens; 66 ducks/pen). Carcass characteristics were evaluated on d 28, 32, and 35. In Experiment 2, 15-d-old ducks (6 replicate cages; 6 ducks/cage) were randomly allotted to the 9 diets that were remixed with 0.5% chromic oxide. Excreta were collected from d 17 to 19, and ileal digesta was collected on d 19 to determine AMEn and amino acid digestibility. In Experiment 1, there were interactions (Pducks were fed a high dietary AMEn (13.75 MJ/kg) and high CP (19%, 1.21% SID Lys). These results provide a framework for subsequent modeling of amino acid and energy inputs and the corresponding outputs of growth performance and carcass components.

  2. Growth performance, carcass characteristics and meat quality of finishing bulls fed crude glycerin-supplemented diets

    Directory of Open Access Journals (Sweden)

    Mônica Chaves Françozo

    2013-04-01

    Full Text Available This study was conducted to evaluate the performance, carcass characteristics and chemical composition of Longissimusmuscle (LM of the bulls. Twenty-four Nellore bulls were used in a complete randomised design. The bulls were randomly assigned to one of the three diets containing 0, 5 or 12% glycerin. Final BW and ADG were similar (P>0.05 between the bulls fed with 5 or 12% of glycerin but were higher (P0.05 by glycerin level. Hot carcass weight increased (P0.05 the conformation, colour, texture, marbling and pH. There was difference (P>0.05 for moisture, ashes and crude protein among glycerin levels. Bulls fed 12% glycerin present the highest (P<0.03 total lipids on LM. The percentage of saturated fatty acids (SFA, monounsaturated acids (MUFA, polyunsaturated acids (PUFA, n-6 and n-3 fatty acids and PUFA/SFA and n-6:n-3 ratios of the LM were similar among the diets. In conclusion, glycerin level did not affect the animal performance and carcass characteristics of Nellore bulls finished in feedlot.

  3. Bioelectrical impedance analysis for the prediction of hot carcass weight in buffalo calf

    Directory of Open Access Journals (Sweden)

    Leopoldo Iannuzzi

    2010-01-01

    Full Text Available Twenty young buffalo male calves were fed ad libitum with a total mix ration and with vitamin-mineral integration for 14 months. Seven days before slaughter, the animals were weighed and bioelectrical impedance measurements were collected in live animals. Physical and chemical characteristics were assessed on the Longissimus dorsi muscle after slaughter. Correlations and regression equations were calculated to determine the possible use of bioelectrical impedance for evaluating hot carcass weight. Bioelectrical impedance analysis at different frequencies, simple correlation and analysis of regression were examined for all the data collected, supporting the possibility of hot carcass weight prediction with equation at multifrequency. The results show that, probably due to the variability in animal live weight, the distribution of the colour parameters was not normally distributed. Moreover, using different frequencies of resistance and reactance, hot carcass weight in buffalo may be predicted with the following equation: Y=98.47–8.84(Rs100KHz+4.41(Rs1000 KHz-116.27(Xc5 KHz+51.04(Xc50 KHz+20.30(Xc100 KHz-33.92(Xc500 KHz+9.01(Xc1000 KHz±ε (Adjusted R Square value of .907 and SE of 5.728 However, further studies are required to improve the technique also in buffalo, after standardization of the method.

  4. Association of myostatin on early calf mortality, growth, and carcass composition traits in crossbred cattle.

    Science.gov (United States)

    Casas, E; Bennett, G L; Smith, T P L; Cundiff, L V

    2004-10-01

    The objective of this study was to investigate a potential association of an inactive myostatin allele with early calf mortality, and evaluate its effect on growth and carcass traits in a crossbred population. Animals were obtained by mating F1 cows to F1 (Belgian Blue x British Breed) or Charolais sires. Cows were obtained from mating Hereford, Angus, and MARC III (1/4 Hereford, 1/4 Angus, 1/4 Pinzgauer, and 1/4 Red Poll) dams to Hereford, Angus, Tuli, Boran, Brahman, or Belgian Blue sires. Belgian Blue was the source of the inactive myostatin allele. Myostatin genotypes were determined for all animals including those that died before weaning. Early calf mortality was examined in the F2 subpopulation (n = 154), derived from the F1 sires mated to F1 cows from Belgian Blue sires, to evaluate animals with zero, one, or two copies of inactive myostatin allele. An overall 1:2:1 ratio (homozygous active myostatin allele:heterozygous:homozygous inactive myostatin allele) was observed in the population; however, a comparison between calves dying before weaning and those alive at slaughter showed an unequal distribution across genotypes (P Carcass composition traits analyzed were hot carcass weight, fat thickness, LM area, marbling score, USDA yield grade, estimated kidney, pelvic, and heart fat, retail product yield and weight, fat yield and weight, bone yield and weight, and percentage of carcasses classified as Choice. Charolais lack the inactive myostatin allele segregating in Belgian Blue; thus, in the population sired by Charolais (n = 645), only animals with zero or one copy of the inactive myostatin allele were evaluated. Animals carrying one copy were heavier at birth and at weaning, and their carcasses were leaner and more muscled. In the population sired by Belgian Blue x British Breed (n = 725), animals with two copies of inactive myostatin allele were heavier at birth, leaner, and had a higher proportion of muscle mass than animals with zero or one copies

  5. ESTIMATION OF GENETIC PARAMETERS FOR CARCASS TRAITS FOR SIMMENTAL CATTLE IN CROATIA

    Directory of Open Access Journals (Sweden)

    Marija Špehar

    2007-06-01

    Full Text Available The objective of this paper was an estimation of genetic parameters for carcass weight, conformation, fatness class and net gain for Simmental cattle in Croatia. Data of young bulls slaughtered in years 2005 and 2006 with 12 to 24 months of age was taken from the central database of the Croatian Livestock Centre. Three data sets were constructed as follows: 1 data set 1 (DS1 included 80462 records of bulls with known and unknown parents; 2 data set 2 (DS2 had 26245 records of bulls with at least one known parents; 3 data set 3 (DS3 with 6272 records of bulls slaughtered between 12 and 14 months of age. Pedigree for DS1 included a total of 118595 animals, 70415 animals and 19319 animals in pedigree for DS2 and DS3, respectively. Variance components were estimated by REML method as implemented in the VCE-5 program package. Statistical model included region, calving season, abattoir-supervisor interaction as fixed class effects and slaughter age as quadratic regression. Direct additive genetic effect was included in the model as random effect. For carcass weight heritability estimates were 0.10 ± 0.006, 0.13 ± 0.02 and 0.11 ± 0.03 for DS1, DS2 and DS3, respectively. Heritability estimates for net gain were 0.09 ± 0.006, 0.16 ± 0.03 and 0.13 ± 0.03, and for carcass conformation were 0.05 ± 0.006, 0.03 ± 0.01 and 0.05 ± 0.006 for DS1, DS2 and DS3, respectively. Results attained indicate that DS2 can be used for genetic evaluation of the Simmental carcass traits in Croatia. However, in the future more improvement should be done, concerning the constitution of contemporary groups, and special attention should be given to data collection.

  6. Genetic effects on carcass quantity, quality, and palatability traits in straightbred and crossbred Romosinuano steers.

    Science.gov (United States)

    Riley, D G; Chase, C C; Coleman, S W; Phillips, W A; Miller, M F; Brooks, J C; Johnson, D D; Olson, T A

    2012-07-01

    The objectives of this work were to estimate heterosis and breed genetic effects for carcass quantity, quality, and palatability traits of steers (Bos spp.) produced from matings of Romosinuano, Brahman, and Angus cattle. Steers (n = 464) were weaned at 7 mo of age and transported to the Southern Great Plains where they grazed winter wheat for 6 mo and were then fed a finishing diet until serial slaughter after different days on feed (average 130 d). Carcass quality and quantity traits were measured; steaks (aged 7 d) were obtained for palatability evaluation. Heterosis was detected for BW, HCW, dressing percentage, LM area, and yield grade for all pairs of breeds. Generally, Romosinuano-Angus heterosis estimates were smallest, Romosinuano-Brahman estimates were intermediate, and Brahman-Angus heterosis estimates were largest. The direct Romosinuano effect was to decrease (P Brahman direct effects were detected for BW (34 ± 17 kg), HCW (29 ± 10 kg), dressing percentage (1.6 ± 0.6 %), LM area per 100 kg HCW (-3.3 ± 0.4 cm(2)/100 kg), and yield grade (0.6 ± 0.1). Significant Angus direct effects were to increase 12th rib fat thickness (3.8 ± 1 mm). Among sire breed means, Romosinuano had reduced (P = 0.002) marbling score (393 ± 9) than Angus, but greater mean sensory tenderness scores (5.8 ± 0.1), and reduced percentage Standard carcasses (10 ± 2%) than Brahman (P carcasses (75 ± 5%) were greater (P Brahman sire breed means (360 ± 11, 5.4 ± 0.1, 31 ± 5%). From consideration only of characteristics of the end product of beef production, Romosinuano did not provide a clearly superior alternative to Brahman for U.S. producers, as they had some quality and palatability advantages relative to Brahman, but at lighter HCW.

  7. Feedlot performance, carcass traits, and palatability traits of Hereford and Hereford x Brahman steers.

    Science.gov (United States)

    Sherbeck, J A; Tatum, J D; Field, T G; Morgan, J B; Smith, G C

    1995-12-01

    Short-yearling steer of known genotypes-straightbred Hereford (100H, n = 80) 75% Hereford x 25% Brahman (75H:25B, n = 80), and 50% Hereford x 50% Brahman (50H:50B, n = 80) were sampled serially at four time-on-feed endpoints (84, 98, 112, 126 d) to compare feedlot performance and carcass and palatability traits of Hereford and Hereford x Brahman steers. After slaughter, USDA yield grade and quality grade factors were recorded, and a portion of the longissimus muscle was removed from the left side of each carcass and fabricated into four 2.54-cm steaks for palatability analyses. Paired steaks from each carcass were aged (6 and 18 d after death), and sensory panel and shear force evaluations were performed. At a constant live weight, 100H steers had higher ADG and produced less mature carcasses with smaller longissimus muscle areas and higher marbling scores than did 75H:25B and 50H:50B steers. The 50H:50B steers had the highest (P Brahman breeding increased. EXtending the postmortem aging period from 6 to 18 d improved shear force values by 20% and panel tenderness ratings by approximately 14%. Beef from steers of the three breeds responded similarly to aging. When Certified Hereford Beef (CHB) specifications were applied, steaks from 100H steers and 75H:25B steers had similar shear force values, suggesting that beef from quarter-blood Brahman crossbred steers could be included in the CHB Program without detrimental effects on product tenderness. PMID:8655435

  8. Feedlot performance, carcass traits, and palatability traits of Hereford and Hereford x Brahman steers.

    Science.gov (United States)

    Sherbeck, J A; Tatum, J D; Field, T G; Morgan, J B; Smith, G C

    1995-12-01

    Short-yearling steer of known genotypes-straightbred Hereford (100H, n = 80) 75% Hereford x 25% Brahman (75H:25B, n = 80), and 50% Hereford x 50% Brahman (50H:50B, n = 80) were sampled serially at four time-on-feed endpoints (84, 98, 112, 126 d) to compare feedlot performance and carcass and palatability traits of Hereford and Hereford x Brahman steers. After slaughter, USDA yield grade and quality grade factors were recorded, and a portion of the longissimus muscle was removed from the left side of each carcass and fabricated into four 2.54-cm steaks for palatability analyses. Paired steaks from each carcass were aged (6 and 18 d after death), and sensory panel and shear force evaluations were performed. At a constant live weight, 100H steers had higher ADG and produced less mature carcasses with smaller longissimus muscle areas and higher marbling scores than did 75H:25B and 50H:50B steers. The 50H:50B steers had the highest (P Brahman breeding increased. EXtending the postmortem aging period from 6 to 18 d improved shear force values by 20% and panel tenderness ratings by approximately 14%. Beef from steers of the three breeds responded similarly to aging. When Certified Hereford Beef (CHB) specifications were applied, steaks from 100H steers and 75H:25B steers had similar shear force values, suggesting that beef from quarter-blood Brahman crossbred steers could be included in the CHB Program without detrimental effects on product tenderness.

  9. Carcass traits of Nellore, F1 Simmental × Nellore and F1 Angus × Nellore steers fed at maintenance or ad libitum with two concentrate levels in the diet

    OpenAIRE

    Ivanna Moraes de Oliveira; Pedro Veiga Rodrigues Paulino; Sebastião de Campos Valadares Filho; Edenio Detmann; Mário Fonseca Paulino; Lúcio Alberto de Miranda Gomide; Marcio de Souza Duarte; Victor Rezende Moreira Couto

    2011-01-01

    This trial was conducted to evaluate the quantitative carcass traits of Nellore, F1 Simmental × Nellore and F1 Angus × Nellore steers fed at maintenance level or ad libitum with 1% and 2% of the body weight in concentrate. The animals were allotted to the treatments in a completely randomized experimental design, in a 3 × 3 factorial arrangement. The animals fed the ad libitum diet with more concentrate had greater cold carcass weight, cold carcass yield and longissimus muscle area/100 kg of ...

  10. The capability of alfalfa grazing- and concentrate-based feeding systems to produce homogeneous carcass quality in light lambs over time

    Directory of Open Access Journals (Sweden)

    Guillermo Ripoll

    2014-02-01

    Full Text Available The effects of grazing on the carcasses and meat of light lambs are unclear, mainly due to variations in weather conditions and pasture production, which affect the growth of lambs and the quality of their carcasses. The aim of this study was to evaluate the effect of feeding systems, which varied in intensification due to the use of concentrate, on the growth and carcass traits of light lambs and the capability of these feeding systems to produce homogeneous lamb carcasses over the course of several years. The average daily weight gain of grazing lambs, but not lambs fed indoors was affected over years. The colour of the Rectus abdominis muscle and the amount of fat were more variable in grazing lambs (from 2.7 to 6.3 than indoor lambs (from 4.5 to 5.1. Grazing feeding systems without concentrate supplementation are more dependent than indoor feeding systems on the year. This climatologic dependence could lead to slaughter of older grazing lambs (77 days to achieve the target slaughter weight when temperatures are low or the rainfall great. All feeding systems evaluated produced light lambs carcasses with a conformation score from O to R- that is required by the market. Even the potential change in fat colour found in both grazing treatments was not enough to change the subjective evaluation of fat colour.

  11. The capability of alfalfa grazing- and concentrate-based feeding systems to produce homogeneous carcass quality in light lambs over time

    Energy Technology Data Exchange (ETDEWEB)

    Ripoll, G.; Alvarez-Rodriguez, J.; Sanz, A.; Joy, M.

    2014-06-01

    The effects of grazing on the carcasses and meat of light lambs are unclear, mainly due to variations in weather conditions and pasture production, which affect the growth of lambs and the quality of their carcasses. The aim of this study was to evaluate the effect of feeding systems, which varied in intensification due to the use of concentrate, on the growth and carcass traits of light lambs and the capability of these feeding systems to produce homogeneous lamb carcasses over the course of several years. The average daily weight gain of grazing lambs, but not lambs fed indoors was affected over years. The colour of the Rectus abdominis muscle and the amount of fat were more variable in grazing lambs (from 2.7 to 6.3) than indoor lambs (from 4.5 to 5.1). Grazing feeding systems without concentrate supplementation are more dependent than indoor feeding systems on the year. This climatologic dependence could lead to slaughter of older grazing lambs (77 days) to achieve the target slaughter weight when temperatures are low or the rainfall great. All feeding systems evaluated produced light lambs carcasses with a conformation score from O to R that is required by the market. Even the potential change in fat colour found in both grazing treatments was not enough to change the subjective evaluation of fat colour. (Author)

  12. The Effect of the Abattoir on Beef Carcass Classification Results

    Directory of Open Access Journals (Sweden)

    Maja Prevolnik

    2011-10-01

    Full Text Available The aim of the present study was to test a possible way of monitoring cattle carcass classification using a statistical approach. For that purpose the analysis of covariance (ANCOVA by SAS was used with the fixed effect of the abattoir, carcass weight (as a covariate and their interaction. The analysis was based on the relationship between carcass weight and conformation or fatness grades. We tested if the regression lines of individual abattoirs differ from the average. The analysis comprised data for young bulls of Simmental breed slaughtered in Slovenia in the period from 2007 to 2010 (52,624 records. Results showed that in many abattoirs the assessment of conformation and fatness deviates significantly from the average, i.e. regression lines for several abattoirs differ significantly from the average (population line. Differences were more important for the conformation than fatness. The statistical process control using the analysis of covariance can be used for additional monitoring of cattle carcass classification.

  13. The Effect of the Abattoir on Beef Carcass Classification Results

    Directory of Open Access Journals (Sweden)

    Maja Prevolnik

    2011-09-01

    Full Text Available The aim of the present study was to test a possible way of monitoring cattle carcass classification using a statistical approach. For that purpose the analysis of covariance (ANCOVA by SAS was used with the fixed effect of the abattoir, carcass weight (as a covariate and their interaction. The analysis was based on the relationship between carcass weight and conformation or fatness grades. We tested if the regression lines of individual abattoirs differ from the average. The analysis comprised data for young bulls of Simmental breed slaughtered in Slovenia in the period from 2007 to 2010 (52,624 records. Results showed that in many abattoirs the assessment of conformation and fatness deviates significantly from the average, i.e. regression lines for several abattoirs differ significantly from the average (population line. Differences were more important for the conformation than fatness. The statistical process control using the analysis of covariance can be used for additional monitoring of cattle carcass classification.

  14. Analysis and Optimisation of Carcass Production for Flexible Pipes

    DEFF Research Database (Denmark)

    Nielsen, Peter Søe

    structure that provides mechanical and collapse strength for the flexible pipe. The manufacturing process of carcass is a combination of roll forming stainless steel strips and helical winding the profiles around a mandrel interlocking the profiles with themselves. The focus of the present project...... is the analysis and optimisation of the carcass manufacturing process by means of a fundamental investigation in the fields of formability, failure modes / mechanisms, Finite Element Analysis (FEA), simulative testing and tribology. A study of failure mechanisms in carcass production is performed by being present...... strip thickness deep. Simulative tribo-testing in the strip-reduction-test showed that biodegradable rapeseed oil is an acceptable lubricant for the carcass process. Testing of two lean duplex stainless steel surfaces showed that a EN 2E brushed surface had better lubricant entrapment capabilities than...

  15. Liver Contaminants in Bald eagles Carcasses from Maine

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Fifty-one livers were extracted from bald eagle carcasses recovered in Maine between 2001 and 2007. Approximately 50% of the birds were collected during the spring...

  16. CARCASS MARKET VALUE OF FATTENER GILTS AND PRIMIPAROUS GILTS

    Directory of Open Access Journals (Sweden)

    Milena BIEGNIEWSKA

    2011-07-01

    Full Text Available Study comprised 60 crossbred gilts F1 (PLW x PL, 30 fattener gilts (A and 30 littermate primiparous gilts (B. The fattening was conducted in standard condition from 30 to 108 kg body weight. The primiparous gilts were mated at second oestrus. During pregnancy and lactation period they were reared in standard condition adopted for pregnant and lactating sows. Fattener sows as well as primiparous sows were slaughtered after end of the experiment. Market carcass value was estimated on carcass jointing basis. It was assumed that market value is a function of percentage share of main parts in carcass and their unitary price (PLN/kg. Market carcass value of fattener sows and primiparous sows counted on 100 kg was comparable.

  17. Animal Carcass Pricing Grid. Evidences from the Romanian Pigmeat Market

    Directory of Open Access Journals (Sweden)

    Savescu Roxana

    2015-07-01

    Full Text Available Until the introduction of SEUROP system, price negotiation between pig producers and slaughterhouses was based on two subjective criteria: animal weight and visual inspection of the quality of pigs. To ensure producers a fair payment, European Union created the possibility of binding the price on two objective factors: carcass weight and carcass composition. This paper describes the pricing mechanism developed by Romania in order to respond to European Union requirements for ensuring a fair payment to pig producers. It raises the attention on the difficulties encountered by the producers in understanding the way the commercial value of a pig carcass is calculated in case the selling price is negotiated on a flat rate basis or on a lean content basis (per kg of carcass weight or per kg of live weight.

  18. Effect of fermented Banana peel on Broiler Carcass

    Directory of Open Access Journals (Sweden)

    Koni TNI

    2013-06-01

    Full Text Available This experiment was conducted to examine effect of inclusion of fermented banana peel by Rhyzopus oligosporus in diets on slaughter weight, carcass weight and carcass percentage, weight and percentage abdominal fat of broiler. The experiment was done based on Completely Randomized Design with four treatments and four replications and each replication consisted of six chickens. The treatment were R0 = without banana peel fermented, R1 = 5% banana peel fermented, R2 = 10% banana peel fermented, R3 = 15% banana peel fermented. Data of the experiment were analyzed, using ANOVA and then continued with Duncan's Multiple Range Test. Result showed that level of fermented banana peel affected slaughter weight and carcass weight. However carcass persentage, weight and percentage of abdominal fat was not affected by treatment. Banana peel fermented by Rhizopus oligosporus could can be used maximally 10% in broiler ration.

  19. Test procedure for the moisture measurement in tyre carcasses

    International Nuclear Information System (INIS)

    The present invention pertains generally to a procedure for the moisture measurement in tyre carcasses with textile insertions by means of neutrons. It allows a permanent control during manufacturing. The invention is applicable to the retreading of tyres

  20. Bacteria recovered from whole-carcass rinsates of broiler carcasses washed in a spray cabinet with lauric acid-potassium hydroxide

    Science.gov (United States)

    The effect of spray washing carcasses with lauric acid (LA)-potassium hydroxide (KOH) on bacteria recovered from whole-carcass-rinsates (WCR) was examined. Skin of carcasses was inoculated with a cecal paste containing antibiotic resistant strains of Escherichia coli, Salmonella Typhimirum, and Camp...

  1. Fixação esquelética externa em cães: avaliação clínica e microbiológica External skeletal fixation in dogs: clinical and microbiological evaluation

    Directory of Open Access Journals (Sweden)

    L.P. Carneiro

    2001-08-01

    Full Text Available Eleven dogs, of both sexes, average weight of 12kg, with ages ranging from two to five years were used with the objective of evaluating two treatments of skin wounds produced after percutaneous bone transfixation of the tibia. In six animals, cotton swabs embedded in 0.2% iodine-alcohol were used to clean the areas of pin perforations. In five dogs, a 0.9% sodium chloride solution, under approximate 30 psi pulse pressure, was used. In both cases, and treatments were carried out every three to four days, during four weeks. Clinical specimens were collected under anaerobic conditions, immediately before the treatments, and on days 3, 7, 14 and 28 after surgery. Samples were cultured in specific media in order to allow isolation of aerobic and anaerobic bacteria and yeast. Dogs treated with sodium chloride solution showed little purulent exudation, while dogs treated with iodine-alcohol presented dry skin wounds with minimum blood-serous to serous exudation. The microbiological evaluation revealed predominance of Staphylococcus spp, but no differences were observed between the two treatments. The results allowed to conclude that both methods are efficient for post-surgical treatments of bone percutaneous transfixation in dogs.Foram utilizados 11 cães de ambos os sexos, sem raça definida, clinicamente sadios, com média de peso de 12kg e idade entre dois e cinco anos, para avaliação de dois métodos de tratamento da ferida cutânea produzida pela transfixação óssea percutânea da tíbia íntegra. Em seis animais empregou-se haste de polipropileno com ponta de algodão embebida em álcool iodado a 0,2% para limpeza dos orifícios de penetração dos pinos. Em cinco cães usou-se soro fisiológico sob pressão pulsátil (± 30 psi. Em ambos os casos, os tratamentos eram repetidos a cada três a quatro dias, por um período de quatro semanas. Espécimes clínicos foram colhidos em anaerobiose após 3, 7, 14 e 28 dias da cirurgia, imediatamente

  2. Dental abscess: A microbiological review

    Directory of Open Access Journals (Sweden)

    Shweta

    2013-01-01

    Full Text Available Dental abscess is a frequently occurring infectious process known to the health practice. The fate of the infection depends on the virulence of the bacteria, host resistance factors, and regional anatomy. Serious consequences arising from the spread of a dental abscess lead to significant morbidity and mortality. Acute dental abscess is polymicrobial, comprising of strict anaerobes, such as anaerobic cocci, Prevotella, Fusobacterium species, and facultative anaerobes, such as viridans group streptococci and the Streptococcus anginosus group. Numerous novel, uncultivable and fastidious organisms have been identified as potential pathogens with the use of non-culture techniques. The majority of localized dental abscesses respond to surgical treatment while the use of antimicrobials is limited to severe spreading infections. There is a need for good-quality clinical trials of sufficient size to identify the ideal treatment. The microbiology of the acute dentoalveolar abscess and its treatment in the light of improved culture and diagnostic methods are reviewed.

  3. Perlemakan pada Sapi Bali dan Sapi Madura Meningkatkan Bobot Komponen Karkas dan Menurunkan Persentase Komponen Nonkarkas. (EFFECT OF BODY FATNESS TO CARCASS AND NON CARCASS PRODUCTIVITY OF SMALL FRAME SIZE BEEF CATTLE (BALI AND MADURA CATTLE

    Directory of Open Access Journals (Sweden)

    Muhammad Ismail

    2014-10-01

    Full Text Available Indonesian has a potentially local beef cattle population but it also has a high slaughtering level ofanimal which tends to increase each years. The main problem of the cattle industry is the diverse conditionof cattle fatness slaughtered in the processing plant. The differences in cattle fatness may influenceproductivity of the local beef cattle. The study was aimed to evaluate the effect of fatness score on carcassand non carcass productivities of small frame size beef cattle. This study used 48 male local beef cattleobtained from eight slaughterhouses from five provinces in Indonesia. The experiment used CompletelyRandomized Design with three level of body fatness that is lean, moderate, and fat. The collected datawere analyzed using analysis of variance and further between treatment differences were tested by DuncanMultiple Range Test. The results showed that fatness score of local beef cattle had significant influence(p<0.05 on slaughter weight, carcass weight, and carcass percentage. The effect of fatness score on weightsand percentages of non carcass components showed varying results. Nevertheless, it was suggested theincreased fatness score would be followed by increased weights and decreased percentages of non carcasscomponents.

  4. THE USE OF ULTRASONOGRAPHY TO PREDICT CARCASS COMPOSITION IN KIDS

    Directory of Open Access Journals (Sweden)

    V. Cadavez

    2007-06-01

    Full Text Available The objective of this work was to analyse the use of ultrasounds to predict carcass composition in kids. Twenty kids from Serrana Portuguese local breed with a mean live weight of 12.6 ± 2.99 kg were scanned by ultrasonography to determine longissimus muscle depth (LD, subcutaneous fat thickness (SF between the 12th and 13th vertebrae (D12, 1st and 2nd (L1 and 3rd and 4th (L3 lumbar vertebra and breast bone tissue thickness at 1st (BT1, 2nd (BT2, 3rd (S3 and 4th (BT4 sternebrae. Lambs were slaughtered after 24-h fasting and carcasses were cooled at 4 ºC for 24 hours. Carcass left side was dissected into muscle, subcutaneous fat, intermuscular fat and bone and remainder (major blood vessels, ligaments, tendons, and thick connective tissue sheets associated with some muscles. Tissues measurements plus hot carcass weight were fitted as independent variables to predict carcass composition by stepwise regression analysis. Models developed 96.7% of muscle, 64.6% of subcutaneous fat, 95.0% of intermuscular fat, and 85.0% of bone weight variation, respectively. Ultrasound measurements were admitted in the models, improving the determination coefficient (R2 and reducing the residual standard deviation. The HCW and tissues measurements taken by ultrasounds in live kids can be used to develop models to predict carcass composition at slaughter-house level.

  5. Necrophagous diptera associated with wild animal carcasses in southern Brazil

    Directory of Open Access Journals (Sweden)

    Ândrio Z. da Silva

    2014-12-01

    Full Text Available Necrophagous Diptera associated with wild animal carcasses in southern Brazil. The aim of this study was to acquire a better knowledge concerning the diversity of necrophagous Diptera that develop on wild animal carcasses. For this purpose, the decomposition of six wild animal carcasses was observed in order to collect and identify the main species of necrophagous flies associated with the decomposition process. The carcasses were found on highways near the cities of Pelotas and Capão do Leão in the initial stage of decomposition, with no significant injuries or prior larval activity. Four wild animal models were represented in this study: two specimens of Didelphis albiventris Lund, 1840; two Tupinambis merianae Linnaeus, 1758; one Nothura maculosa Temminck, 1815; and one Cerdocyon thous Linnaeus, 1766. A total of 16,242 flies from 14 species were reared in the laboratory, where Muscidae presented the greatest diversity of necrophagous species. Overall, (i carcasses with larger biomass developed a higher abundance of flies and (ii the necrophagous community was dominated by Calliphoridae, two patterns that were predicted from published literature; and (iii the highest diversity was observed on the smaller carcasses exposed to the lowest temperatures, a pattern that may have been caused by the absence of the generalist predator Chrysomya albiceps (Wiedemann, 1819. (iv An UPGMA analysis revealed a similar pattern of clusters of fly communities, where the same species were structuring the groupings.

  6. Nutritional, immunological and microbiological profiles of burn patients

    Directory of Open Access Journals (Sweden)

    ASAA Barbosa

    2009-01-01

    Full Text Available A burn is a lesion on an organic tissue resultant from direct or indirect action of heat on the organism. The present study aimed to evaluate the nutritional, immunological and microbiological status of burn patients at the Bauru State Hospital, São Paulo state, Brazil, in 2007. Eight patients, aged more than 18 years and injured up to 24 hours, were evaluated at the moment of hospitalization and seven days later. All victims were males with a mean age of 38 years. On average, 17.5% of their body surfaces were burned and 50% of the patients were eutrophic. There were significant alterations in levels of erythrocytes, hemoglobin, hematocrit, total protein and albumin due to increased endothelial permeability, direct destruction of proteins in the heat-affected area and blood loss from lesions or debridement. At a second moment, cytokines IL-6 and TNF-α had augmented significantly, with IL-6 presenting elevated levels in relation to controls at the first moment. Microbiological analysis showed that 100% of the samples collected at hospital admission were negative and after one week Staphylococcus aureus was found in all cultures. Therefore, a burn patient may be considered immunosuppressed and these results indicate significant nutritional, immunological and microbiological alterations that can interfere in his recovery.

  7. Microbiological toxicity of tilmicosin on human colonic microflora in chemostats.

    Science.gov (United States)

    Hao, Haihong; Yao, Junping; Wu, Qinghua; Wei, Yajing; Dai, Menghong; Iqbal, Zahid; Wang, Xu; Wang, Yulian; Huang, Lingli; Chen, Dongmei; Tao, Yanfei; Liu, Zhenli; Yuan, Zonghui

    2015-10-01

    To evaluate the microbiological safety of tilmicosin on human intestinal microflora, four chemostat models of healthy human colonic ecosystems were exposed to tilmicosin (0, 0.436, 4.36, and 43.6 μg/mL) for 7 days. Prior to and during drug exposure, three microbiological endpoints were monitored daily including short-chain fatty acids, bacterial counts and macrolide susceptibility. Colonization resistance of each community was determined by 3 successive daily challenges of Salmonella typhimurium. Genes associated with virulence and macrolide resistance in Enterococcus faecalis were determined by PCR. Transcriptional expression of the virulence gene (gelE) in E. faecalis was determined by real-time RT-PCR. Our results showed that different concentrations of tilmicosin did not disrupt the colonization resistance in each chemostat. During exposure to 4.36 and 43.6 μg/mL tilmicosin, the Bacteroides fragilis population was significantly decreased while the proportion of resistant Enterococci increased. After long-term exposure to the highest concentration (43.6 μg/mL) of tilmicosin, the gelE gene was significantly up-regulated in the high-level macrolide resistant strains that also contained the ermB resistance gene. This study was the first of its kind to evaluate the microbiological toxicity of tilmicosin using a chemostat model. These findings also provide new insight into the co-occurrence of macrolide resistance and virulence in E. faecalis under tilmicosin selective pressure.

  8. Effects of feeding a return chewing gum/packaging material mixture on performance and carcass characteristics of feedlot cattle.

    Science.gov (United States)

    Wolf, B W; Berger, L L; Fahey, G C

    1996-11-01

    Seventy-two Simmental-cross growing steers (219 +/- 2.4 kg initial BW) were used in a randomized complete block design to evaluate the effects of feeding a return chewing gum/packaging material mixture (G/P) on feedlot performance, carcass characteristics, sensory attributes of meat, and mineral content of beef liver and muscle. Animals were allotted by weight to 12 pens (six/pen). Each pen was assigned one of three dietary treatments: 1) 0% G/P (control), 2) 20% G/P, or 3) 30% G/P (% G/P on a DM basis). Steers were fed their respective diets for an 84-d growing phase and a 112-d finishing phase. The G/P replaced corn silage and corn in the growing and finishing phases, respectively. Eighteen steers (six/treatment) were randomly selected for slaughter at the end of the finishing phase, and carcass measurements, sensory attributes of meat, and mineral content of liver and longissimus muscle were measured. During the growing phase, steers fed G/P-containing diets had improved (P .05) on carcass characteristics. Steaks from steers fed 20% G/P had improved (P carcass merit. PMID:8923170

  9. Relative growth of carcass tissues of goat kids from five breed Types finished on pasture or feedlot

    Directory of Open Access Journals (Sweden)

    Raquel Vasconcelos Lourençon

    2016-04-01

    Full Text Available The aim of this study was to evaluate the effect of cross breeding, finishing system, and gender on the relative growth of carcass tissues of dairy kids. Seventy eight kids (39 male and 39 female from five breed types were used: Alpine; ½ Boer + ½ Alpine (½ BA; ½ Nubian + ½ Alpine (½ ANA; ¾ Boer + ¼ Alpine (¾ BA; and ½ Nubian + ¼ Boer + ¼ Alpine (TC. Kids were distributed into two finishing systems: in pasture with doe (FS1 and weaned in feedlot (FS2. Kids were slaughtered at a mean age of 128.4 ± 7.9 days and mean live weight of 22.07 kg. The mean weight of half carcasses was 5.09 kg. To determine allometric growth, we used the exponential equation Y= aXb. In the half carcass, muscle tissue showed comparatively early growth in group ½ BA, whereas fat tissue of animals in FS1 had relatively late growth. Females exhibited early growth of muscle tissue, while in males this tissue was intermediate. The ½ BA first-cross improved carcass characteristics by enhancing the growth of muscle tissue.

  10. Antimicrobial susceptibility of Salmonella serovars isolated from edible offal and carcasses of slaughtered poultry in the state of Tocantins, Brazil

    Directory of Open Access Journals (Sweden)

    Silvia Minharro

    2015-08-01

    Full Text Available The aim of this study was to evaluate the profile of antibiotic resistance in Salmonella isolated from the carcasses, livers and hearts of chickens slaughtered in the state of Tocantins, Brazil, as recommended by the Normative Instruction 70 of 2003 of the Ministry of Agriculture, Livestock and Food Supply and the National Monitoring Program Prevalence and Bacterial Resistance in Chicken. Carcasses, livers and hearts from chicken with or without pericarditis/perihepatitis were studied in 60 lots of poultry slaughtered under the Federal Inspection System in the state of Tocantins, Brazil between August 2010 and June 2011. Twenty-six indicative Salmonella sp. were isolated in 11 lots (18.33%. Different strains of Salmonella were isolated more than a kind of sample/lot. The most frequent serovar was Enteriditis (38.46%, 10/26, while the second was Mbandaka (19.23%, 5/26, both isolated from hearts, livers and carcasses. Regarding antimicrobial resistance, of 12 tested principal pharmacological agents, the samples appeared to be most sensitive to tetracyclines, but showed 100% resistance to one or more active principal agents, especially sulfamethoxazole (30 mcg and amoxicillin/clavulanic acid (30 mcg. Although Salmonella sp. was isolated from normal carcasses, the results are within permitted levels for unfrozen products according to Brazilian legislation. However, one should always be aware of the hygienic and sanitary conditions of production processes and food processing, especially regarding good manufacturing practices.

  11. The effects of different sizes of insoluble grit on growth performance and carcass traits in broiler chickens

    Directory of Open Access Journals (Sweden)

    Abbas Ali Rajabiyan Moghaddam

    2016-04-01

    Full Text Available The present study was conducted to investigate the effects of different sizes of insoluble grit on growth performance and carcass traits in broiler chickens. A total of 200 broilers (Ross 308, 10 days old, were randomly allotted to five experimental equal groups with four replicates of 10 chickens (five male and five female and fed with basal diet + ground wheat (without grit; basal diet + whole wheat (without grit and basal diet contain 1.5% grit of diet with sizes of 2, 3 and 4 mm. Growth performance (evaluated through weight gain, feed intake and feed conversion ratio was determined on day 24 and 42. Also, carcass traits (relative weights of carcass, breast, thigh, liver, heart, gizzard and intestine and intestine length were assessed on day 42. Weight gains and feed conversion ratio were significantly improved in broilers added with grit 2 mm compared to the control group (p<0.05, whereas; carcass traits were not significantly altered. These data suggest that grit with size of 2 mm improve growth performance in broiler chickens.

  12. Partial Replacement of Ground Corn with Glycerol in Beef Cattle Diets: Intake, Digestibility, Performance, and Carcass Characteristics.

    Directory of Open Access Journals (Sweden)

    Pedro Del Bianco Benedeti

    Full Text Available The objective of this study was to evaluate the effects of replacing dry ground corn with crude glycerol on intake, apparent digestibility, performance, and carcass characteristics of finishing beef bulls. A completely randomized block design experiment with 25 d for adaptation and 100 d for data collection was conducted, in which 3,640 Nellore bulls (367 ± 36.8 kg; 18 ± 3 mo were blocked by body weight and assigned to 20 pens. Bulls were randomly assigned to one of four treatments: 0, 5, 10, and 15% (dry matter basis of crude glycerol in the diet. Initially, 20 bulls were slaughtered to serve as a reference to estimate initial empty body weight, which allowed for carcass gain calculation. Bulls were weighed at the beginning, at two-thirds, and at the end of the experiment for performance calculations. Carcass measurements were obtained by ultrasound. Fecal output was estimated using indigestible neutral detergent fiber as an internal marker. Data were analyzed using the mixed procedures in SAS 9.2 (SAS Institute Inc., Cary, NC. Intake of dry matter, organic matter, and neutral detergent fiber decreased linearly (P 0.05 intakes of crude protein, non-fiber carbohydrates, and total digestible nutrients. Digestibility of dry matter, organic matter, neutral detergent fiber, and total digestible nutrients increased quadratically (P 0.05. These results suggest that crude glycerol may be included in finishing beef diets at levels up to 15% without impairing performance and carcass characteristics.

  13. Carcass characteristics and meat quality of Santa Inês lambs fed with sunflower cake replacing cottonseed meal

    Directory of Open Access Journals (Sweden)

    Francisco Fernandes Júnior

    2014-02-01

    Full Text Available The objective of this study was to evaluate carcass characteristics and meat quality of lambs fed sunflower cake to replace the protein of cottonseed meal. The experiment was conducted at the sheep sector at the Farm School, State University of Londrina. Thirty Santa Inês lambs, intact males, with age and weight at the beginning of the experiment of 80 days and 21.45 ± 2.16 kg, respectively, were used. It was used a completely randomized experimental design. The animals were divided into 5 treatments, with 6 replicates per treatment, according to the amount of protein of cottonseed meal replaced by protein of the sunflower cake (0, 20, 40, 60 and 80% of replacement. Carcasses were evaluated and the Longissimus dorsi was removed for analyzes of shear force, color, pH, marbling, water loss by pressure, sensory analysis, chemical analyses and lipid oxidation. Hot and cold carcass yields showed quadratic distributions, where the best values were found for treatments with low inclusion of sunflower cake. Carcass measurements, as well as cut yields were not affected by the content of sunflower cake. Regarding the parameters of lamb meat, only water losses during cooking and shear force were affected, being quadratic and linear, respectively. The meat was more tender in animals fed higher amounts of sunflower cake. The tasters of the sensory panel did not identify differences between treatments, and classified the meat with moderate odor intensity, good tenderness, low to moderate juiciness and medium acceptability. The replacement of cottonseed meal protein by sunflower cake protein in the levels used, did not affect the majority of the carcass characteristics and the meat quality.

  14. Automation in the clinical microbiology laboratory.

    Science.gov (United States)

    Novak, Susan M; Marlowe, Elizabeth M

    2013-09-01

    Imagine a clinical microbiology laboratory where a patient's specimens are placed on a conveyor belt and sent on an automation line for processing and plating. Technologists need only log onto a computer to visualize the images of a culture and send to a mass spectrometer for identification. Once a pathogen is identified, the system knows to send the colony for susceptibility testing. This is the future of the clinical microbiology laboratory. This article outlines the operational and staffing challenges facing clinical microbiology laboratories and the evolution of automation that is shaping the way laboratory medicine will be practiced in the future.

  15. AVALIAÇÃO MICROBIOLÓGICA DA CARNE-DE-SOL ELABORADA COM BAIXOS TEORES DE CLORETO DE SÓDIO MICROBIOLOGICAL EVALUATION OF SUN DRIED MEAT WITH LOW SODIUM CHLORIDE

    Directory of Open Access Journals (Sweden)

    Elizabete Lourenço da COSTA

    2001-08-01

    Full Text Available Foram analisadas 96 amostras de carne-de-sol, das quais 48 foram coletadas em estabelecimentos inspecionados e 48 em estabelecimentos não-inspecionados. Com o objetivo de avaliar as condições higiênico-sanitárias desse produto, durante o processamento e a comercialização, pesquisou-se a incidência de bactérias mesófilas, bolores e leveduras, Staphylococcus aureus, coliformes fecais e Escherichia coli. Foram analisados ainda, alguns parâmetros físico-químicos como atividade de água e teor de cloreto de sódio. Nos dois grupos os resultados obtidos nas análises microbiológicas foram bastante elevados. Nas amostras provenientes de estabelecimentos inspecionados as médias da contagem total de bactérias mesófilas, bolores e leveduras, Staphylococcus spp e coliformes fecais foram 6,2Log UFC/g, 3,8Log UFC/g, 7,4Log UFC/g e 1,3Log NMP/g, respectivamente, e, nas comercializadas sem inspeção foram 7,4Log UFC/g, 4,4Log UFC/g, 6,8Log UFC/g e 2,6Log NMP/g, respectivamente. Esses resultados sugerem que houve falhas tanto no processamento como na manipulação desses produtos, sendo que a contaminação fecal foi bem mais elevada nas amostras coletadas nos estabelecimentos sem inspeção. A variação dos teores de cloreto de sódio e atividade de água das amostras indicou falta de padronização de qualidade da carne-de-sol, havendo a necessidade de se estabelecer parâmetros legais tanto microbiológicos como físico-químicos, capazes de oferecer ao consumidor um alimento seguro.A total of 96 samples of sun meat were analyzed, 48 of them were collected in inspected establishments and the other 48 in establishments without inspection. This work was developed in order to evaluate the hygienic-sanitary conditions of these products during their processing and marketing. The incidence of mesophilic bacterias, molds, yeasts, Staphylococcus aureus, fecal coliforms and Escherichia coli was investigated. Also, some physical-chemical parameters

  16. Hazardous animal waste carcasses transformation into slow release fertilizers.

    Science.gov (United States)

    Sharrock, Patrick; Fiallo, Marina; Nzihou, Ange; Chkir, Mouna

    2009-08-15

    Because of the need to address disposal of materials infected with pathogens new regulations have come into effect for the transport and disposal of dead farm animals or carcasses. For precautionary reasons, disposal to landfill, composting, biogas generation or fertilizer use are banned recycling paths because of incomplete knowledge about contamination transmission paths. Thermal treatment is recognized as a safe elimination process. Animal wastes have a high calorific value (above 16 MJ/kg). However, combustion of the organics leaves mineral residues (near 30%). The ashes contain mostly calcium and phosphate with some sodium, potassium and magnesium. We have examined the transformation of the ashes into a slow release fertilizer. We used a mixture of acids to partly dissolve the combustion residues and form slurry. In a second step, base was added to neutralize and solidify the reaction mixture. The final product was a whitish polycrystalline solid. Leaching tests were made to evaluate the nutrient release rate in laboratory columns. Water leachates were analyzed for up to ten pore-bed volumes and showed, as expected, large differences in release rates. Nitrate release was slowed and phosphate did not level even after ten pore-bed volumes. This demonstrates that insoluble precipitates (gypsum) contribute to control soluble ion release. PMID:19185419

  17. Influence of Feed Additives in Quality of Broiler Carcasses

    Directory of Open Access Journals (Sweden)

    F.A. Khalafalla

    2012-09-01

    Full Text Available This study was carried out to assess the effect of some feed additives on quality of broiler carcasses. A total of one hundred and eighty of one day old broiler chicks were reared and divided into six groups. Five groups were fed on treated rations (20 g of freshly minced of each of garlic and onion to 1 Kg of ration, 400mg of vitamin E in one liter of drinking water, B.subtilis 4 ×108 c.f.u was added in amount 1.5 g to 1 Kg ration, 10 g hot pepper to one Kg of ration and 50g zinc bacitracin added to the ration in amount 1.5 g to 1 Kg ration, and the sixth group used as control group. Broilers were slaughtered at age of 45 days to evaluate pH, moisture content, cooking loss, shear force and instrumental color. The feed additives (onion and garlic, Vit.E, hot pepper, B.subtilis and zinc bacitracin were decreased pH in broiler meat. Moisture did not influence by dietary supplementation. Cooking loss was decreased with storage of broiler meat. Vit.E, B.subtilis and hot Pepper groups increased tenderness in broiler meat. Onion and garlic and Vit.E increased lightness and yellowness of broiler meat. Zinc bacitracin and hot pepper decreased redness of broiler meat.

  18. Microbiological profile of selected mucks

    Science.gov (United States)

    Dąbek-Szreniawska, M.; Wyczółkowski, A. I.

    2009-04-01

    INTRODUCTION Matyka-Sarzynska and Sokolowska (2000) emphasize that peats and peat soils comprise large areas of Poland. The creation of soil begins when the formation of swamp has ended. Gawlik (2000) states that the degree of influence of the mucky process of organic soils on the differentiations of the conditions of growth and development of plants is mainly connected with the changes of moisture-retentive properties of mucks which constitute the material for these soils, and the loss of their wetting capacities. The above-mentioned changes, which usually occur gradually and show a clear connection with the extent of dehydration and, at times, with its duration, intensify significantly when the soils are under cultivation. The mucky process of peat soils leads to transformations of their physical, chemical and biological properties. The main ingredient of peat soils is organic substance. The substance is maintained inside them by the protective activity of water. The process of land improvement reduces the humidity of the environment, and that Intensifies the pace of the activity of soil microorganisms which cause the decay of organic substance. The decay takes place in the direction of two parallel processes: mineralization and humification. All groups of chemical substances constituting peat undergo mineralization. Special attention should be called to the mineralization of carbon and nitrogen compounds, which constitute a large percentage of theorganic substance of the peat organic mass. Okruszko (1976) has examined scientificbases of the classification of peat soils depending on the intensity of the muck process. The aim of this publication was to conduct a microbiological characteristic of selected mucky material. METHODS AND MATERIALS Soil samples used in the experiments were acquired from the Leczynsko-Wlodawski Lake Region, a large area of which constitutes a part of the Poleski National Park, which is covered to a large extent with high peat bogs. It was

  19. Seasonal variation exceeds effects of salmon carcass additions on benthic food webs in the Elwha River

    Science.gov (United States)

    Morley, S.A.; Coe, H.J.; Duda, J.J.; Dunphy, L.S.; McHenry, M.L.; Beckman, B.R.; Elofson, M.; Sampson, E. M.; Ward, L.

    2016-01-01

    Dam removal and other fish barrier removal projects in western North America are assumed to boost freshwater productivity via the transport of marine-derived nutrients from recolonizing Pacific salmon (Oncorhynchus spp.). In anticipation of the removal of two hydroelectric dams on the Elwha River in Washington State, we tested this hypothesis with a salmon carcass addition experiment. Our study was designed to examine how background nutrient dynamics and benthic food webs vary seasonally, and how these features respond to salmon subsidies. We conducted our experiment in six side channels of the Elwha River, each with a spatially paired reference and treatment reach. Each reach was sampled on multiple occasions from October 2007 to August 2008, before and after carcass placement. We evaluated nutrient limitation status; measured water chemistry, periphyton, benthic invertebrates, and juvenile rainbow trout (O. mykiss) response; and traced salmon-derived nutrient uptake using stable isotopes. Outside of winter, algal accrual was limited by both nitrogen and phosphorous and remained so even in the presence of salmon carcasses. One month after salmon addition, dissolved inorganic nitrogen levels doubled in treatment reaches. Two months after addition, benthic algal accrual was significantly elevated. We detected no changes in invertebrate or fish metrics, with the exception of 15N enrichment. Natural seasonal variability was greater than salmon effects for the majority of our response metrics. Yet seasonality and synchronicity of nutrient supply and demand are often overlooked in nutrient enhancement studies. Timing and magnitude of salmon-derived nitrogen utilization suggest that uptake of dissolved nutrients was favored over direct consumption of carcasses. The highest proportion of salmon-derived nitrogen was incorporated by herbivores (18–30%) and peaked 1–2 months after carcass addition. Peak nitrogen enrichment in predators (11–16%) occurred 2–3

  20. Genetic parameter estimates for carcass traits and visual scores including or not genomic information.

    Science.gov (United States)

    Gordo, D G M; Espigolan, R; Tonussi, R L; Júnior, G A F; Bresolin, T; Magalhães, A F Braga; Feitosa, F L; Baldi, F; Carvalheiro, R; Tonhati, H; de Oliveira, H N; Chardulo, L A L; de Albuquerque, L G

    2016-05-01

    The objective of this study was to determine whether visual scores used as selection criteria in Nellore breeding programs are effective indicators of carcass traits measured after slaughter. Additionally, this study evaluated the effect of different structures of the relationship matrix ( and ) on the estimation of genetic parameters and on the prediction accuracy of breeding values. There were 13,524 animals for visual scores of conformation (CS), finishing precocity (FP), and muscling (MS) and 1,753, 1,747, and 1,564 for LM area (LMA), backfat thickness (BF), and HCW, respectively. Of these, 1,566 animals were genotyped using a high-density panel containing 777,962 SNP. Six analyses were performed using multitrait animal models, each including the 3 visual scores and 1 carcass trait. For the visual scores, the model included direct additive genetic and residual random effects and the fixed effects of contemporary group (defined by year of birth, management group at yearling, and farm) and the linear effect of age of animal at yearling. The same model was used for the carcass traits, replacing the effect of age of animal at yearling with the linear effect of age of animal at slaughter. The variance and covariance components were estimated by the REML method in analyses using the numerator relationship matrix () or combining the genomic and the numerator relationship matrices (). The heritability estimates for the visual scores obtained with the 2 methods were similar and of moderate magnitude (0.23-0.34), indicating that these traits should response to direct selection. The heritabilities for LMA, BF, and HCW were 0.13, 0.07, and 0.17, respectively, using matrix and 0.29, 0.16, and 0.23, respectively, using matrix . The genetic correlations between the visual scores and carcass traits were positive, and higher correlations were generally obtained when matrix was used. Considering the difficulties and cost of measuring carcass traits postmortem, visual scores of

  1. Prescott’s Microbiology, Eighth Edition

    OpenAIRE

    Joanne J. Dobbins

    2010-01-01

    Review of: Prescott’s Microbiology, Eighth Edition. Joanne M. Willey, Linda M. Sherwood, and Christopher J. Woolverton. 2011. McGraw-Hill Higher Education, NewYork, NY. 1070 pages. ISBN- 978-0-07-337526-7.

  2. Effects of ractopamine supplementation on growth performance and carcass characteristics of feedlot steers differing in biological type.

    Science.gov (United States)

    Gruber, S L; Tatum, J D; Engle, T E; Mitchell, M A; Laudert, S B; Schroeder, A L; Platter, W J

    2007-07-01

    Effects of ractopamine hydrochloride (RAC) supplementation on growth performance and carcass characteristics of feedlot steers differing in biological type were investigated using British, Continental crossbred, and Brahman crossbred calf-fed steers (n = 420). Steers of each type were weighed at reimplantation [British, mean BW = 375 kg (SD = 38 kg); Continental crossbred, mean BW = 379 kg (SD = 42 kg); Brahman crossbred, mean BW = 340 (SD = 32 kg)] and sorted into 7 BW blocks, each block consisting of 2 pens (10 steers per pen) per type. Pens within a block x type subclass were randomly assigned to RAC treatments (0 or 200 mg x steer(-1) x d(-1) fed during the final 28 d of the finishing period). The type x RAC interaction did not affect (P > 0.05) any of the traits evaluated in this study. Feeding RAC improved (P = 0.001) ADG (1.50 vs. 1.73 +/- 0.09 kg) and G:F (0.145 vs. 0.170 +/- 0.005), but did not affect (P = 0.48) DMI of steers. Dressing percentage, adjusted fat thickness, KPH percentage, and yield grade were not affected by RAC supplementation. Carcasses of steers fed RAC had heavier (P = 0.01) HCW (359 vs. 365 +/- 4.9 kg), larger (P = 0.046) LM areas (81.7 vs. 84.0 +/- 1.1 cm(2)), and tended (P = 0.07) to have lower mean marbling scores (487 vs. 477 +/- 5.2; Slight = 400, Small = 500) than did carcasses of control steers. Among the 3 biological types, Brahman crossbred steers had the lowest DMI and produced the lightest-weight carcasses that had the lowest mean marbling score (P Brahman crossbred steers, British steers produced carcasses with the greatest (P = 0.001) mean marbling scores. Continental crossbred steers had the heaviest BW and greatest dressing percentages and produced the heaviest carcasses with the largest LM areas (P Brahman crossbred steers. In the present study, 28 d of supplementation with RAC at a dosage rate of 200 mg x steer(-1) x d(-1) elicited consistent responses in growth performance and carcass traits among 3 diverse biological

  3. Effects of ractopamine supplementation on growth performance and carcass characteristics of feedlot steers differing in biological type.

    Science.gov (United States)

    Gruber, S L; Tatum, J D; Engle, T E; Mitchell, M A; Laudert, S B; Schroeder, A L; Platter, W J

    2007-07-01

    Effects of ractopamine hydrochloride (RAC) supplementation on growth performance and carcass characteristics of feedlot steers differing in biological type were investigated using British, Continental crossbred, and Brahman crossbred calf-fed steers (n = 420). Steers of each type were weighed at reimplantation [British, mean BW = 375 kg (SD = 38 kg); Continental crossbred, mean BW = 379 kg (SD = 42 kg); Brahman crossbred, mean BW = 340 (SD = 32 kg)] and sorted into 7 BW blocks, each block consisting of 2 pens (10 steers per pen) per type. Pens within a block x type subclass were randomly assigned to RAC treatments (0 or 200 mg x steer(-1) x d(-1) fed during the final 28 d of the finishing period). The type x RAC interaction did not affect (P > 0.05) any of the traits evaluated in this study. Feeding RAC improved (P = 0.001) ADG (1.50 vs. 1.73 +/- 0.09 kg) and G:F (0.145 vs. 0.170 +/- 0.005), but did not affect (P = 0.48) DMI of steers. Dressing percentage, adjusted fat thickness, KPH percentage, and yield grade were not affected by RAC supplementation. Carcasses of steers fed RAC had heavier (P = 0.01) HCW (359 vs. 365 +/- 4.9 kg), larger (P = 0.046) LM areas (81.7 vs. 84.0 +/- 1.1 cm(2)), and tended (P = 0.07) to have lower mean marbling scores (487 vs. 477 +/- 5.2; Slight = 400, Small = 500) than did carcasses of control steers. Among the 3 biological types, Brahman crossbred steers had the lowest DMI and produced the lightest-weight carcasses that had the lowest mean marbling score (P Brahman crossbred steers, British steers produced carcasses with the greatest (P = 0.001) mean marbling scores. Continental crossbred steers had the heaviest BW and greatest dressing percentages and produced the heaviest carcasses with the largest LM areas (P Brahman crossbred steers. In the present study, 28 d of supplementation with RAC at a dosage rate of 200 mg x steer(-1) x d(-1) elicited consistent responses in growth performance and carcass traits among 3 diverse biological

  4. Clinical and economic evaluation of BBL CHROMagar Salmonella (CHROMSal) versus subculture after selenite broth enrichment to CHROMSal and Hektoen enteric agars to detect enteric Salmonella in a large regional microbiology laboratory.

    Science.gov (United States)

    Church, Deirdre L; Emshey, Diana; Lloyd, Tracie; Pitout, Johann

    2010-09-01

    Stool culture for enteric pathogens is one of the most labor-intensive clinical microbiology procedures. Direct plating of stool to BBL CHROMagar Salmonella (CHROMSal) (BD Diagnostics, Sparks, MD) versus subculture after selenite broth enrichment (Sel) to CHROMSal (Sel-CHROMSal) and Hektoen enteric agar (Sel-Hek) (PML Microbiologicals, Eugene, OR) to detect Salmonella were compared. The number of colony picks and biochemical/serotyping tests per plate was recorded. A cost comparison was done. Fifty-one of 2999 (1.7%) stools yielded Salmonella sp., and 80% of isolates grew on CHROMSal by 24 h. CHROMSal demonstrated much less false-positive growth compared to Sel-Hek (P < 0.0001), which reduced biochemical and serotyping tests by 85% and 20%, respectively. Sel-CHROMSal and CHROMSal versus Sel-Hek improved enteric Salmonella detection when compared to a true positive "gold standard" (i.e., recovery by any culture method) with a sensitivity, specificity, positive predictive value, and negative predictive value of 100% and 94.12%, 100% and 99.97%, 100% and 97.96%, and 100% and 99.90%, respectively. CHROMSal use would result in substantial cost and labor savings.

  5. Microbiology facilities aboard Space Station Freedom (SSF)

    Science.gov (United States)

    Cioletti, L. A.; Mishra, S. K.; Richard, Elizabeth E.; Taylor, R.

    1990-01-01

    A comprehensive microbiological facility is being designed for use on board Space Station Freedom (SSF). Its purpose will be to conduct microbial surveillance of the SSF environment and to examine clinical specimens. Air, water, and internal surfaces will be periodically monitored to satisfy requirements for a safe environment. Crew health will remain a principle objective for every mission. This paper will review the Microbiology Subsystem capabilities planned for SSF application.

  6. Transfer of Campylobacter from a Positive Batch to Broiler Carcasses of a Subsequently Slaughtered Negative Batch: A Quantitative Approach.

    Science.gov (United States)

    Seliwiorstow, Tomasz; Baré, Julie; Van Damme, Inge; Gisbert Algaba, Ignacio; Uyttendaele, Mieke; De Zutter, Lieven

    2016-06-01

    The present study was conducted to quantify Campylobacter cross-contamination from a positive batch of broiler chicken carcasses to a negative batch at selected processing steps and to evaluate the duration of this cross-contamination. During each of nine visits conducted in three broiler slaughterhouses, Campylobacter levels were determined on broiler carcasses originating from Campylobacter-negative batches processed immediately after Campylobacter-positive batches. Data were collected after four steps during the slaughter process (scalding, plucking, evisceration, and washing) at 1, 10, and 20 min after the start of the slaughter of the batches. Campylobacter levels in ceca of birds from Campylobacter-positive batches ranged from 5.62 to 9.82 log CFU/g. When the preceding positive batch was colonized at a low level, no (enumerable) carcass contamination was found in a subsequent negative batch. However, when Campylobacter levels were high in the positive batch, Campylobacter was found on carcasses of the subsequent negative batch but at levels significantly lower than those found on carcasses from the preceding positive batch. The scalding and the evisceration process contributed the least (< 1.5 log CFU/g) and the most (up to 4 log CFU/ g), respectively, to the Campylobacter transmission from a positive batch to a negative batch. Additionally, the number of Campylobacter cells transferred from positive to negative batches decreased over the first 20 min of sampling time. However, the reduction was slower than previously estimated in risk assessment studies, suggesting that pathogen transfer during crosscontamination is a complex process.

  7. Qualitative aspects of the carcass and meat of young steers Devon finished in tropical pasture, receiving different levels of concentrate

    Directory of Open Access Journals (Sweden)

    Luis Fernando Glasenapp de Menezes

    2014-06-01

    Full Text Available The objective of this work was to evaluate the carcass and meat characteristics of young steers Devon, finished in tropical pasture (association of millet pasture - Pennisetum americanum (L. Leeke and Alexander grass – Brachiaria plantaginea, receiving different levels of supplementation (0; 0,5% or 1,0% of body weight. The animals had an average initial weight of 300 kg and 15 months of age, and were slaughtered at an average weight of 353.0 kg. Supplementation with concentrate ration to the level of 1.0% of body weight (BW of animal provided carcasses with better quality (higher hot and cold carcass, and greater fat thickness, which resulted in lower cooling loss; carcasses more compact (less long, with better conformation and thicker cushion when compared to non-supplemented. Furthermore, animals fed only pasture, showed a lower percentage of carcasses forequarter (37.6 vs 38.9 e 38.8%, with a higher percentage of muscle (65.5 vs 59.6 e 59.5 %, which resulted in greater relative muscle tissue: bone and edible portion: bone than those supplemented. As the sensory and organoleptic qualities, it was found that meat from animals supplemented with up to 1.0% of body weight, showed greater losses to cooking (24.1 % vs 18.0 % and required greater force to shear the fiber (3.37 vs 2.05 (kgf/ cm3 compared to meat non-supplemented animals. However, the increased level of supplementation of 0.5% to 1.0% of BW improved palatability and reduced meat texture.

  8. Carcass and meat characteristics of steers or bulls, finished in feedlot and fed with diets containing three levels of concentrate

    Directory of Open Access Journals (Sweden)

    José Luis Moletta

    2014-02-01

    Full Text Available The objective of this experiment was to evaluate quantitative and qualitative characters of carcass and meat from bulls and steers finished in feedlot during a 116 day period, in individual stalls and fed with a diet of corn silage and three levels of concentrate (0.70; 0.97 and 1.23% of body weight. The concentrate was formulated with 25% soybean meal, 73% of ground corn grain 1% of a mineral mix and 1% of limestone. A total of 169 composite Purunã animals, being 94 bulls and 75 steers with average age of 20 months. The interaction between physiological condition and level of concentrate was not significant (P>0.05 for any of the studied variables. Likewise, no effect of level of concentrate was detected upon the same traits. The bulls presented heavier slaughter weight (493.1 kg in comparison with the steers (450.0 kg as well as higher carcass yield (55.2 vs. 53.5%, resulting in a hot carcass weight 12.1% heavier than bulls animals, though with a lower degree of carcass finishing (3.6 vs. 4.2 mm, respectively. Bulls produced carcasses with better conformation and higher area of Longissimus dorsi (68.6 vs. 63.3 cm2, and higher percentage of muscle in comparison with castrated (66.5 vs. 62.8%. The meat from bulls was darker and with lower degree of marbling in relation to steers. Nevertheless, no differences were observed for tenderness nor for juiciness, though steers had more palatable meat.

  9. Microbiological characterization of deep geological compartments

    International Nuclear Information System (INIS)

    Document available in extended abstract form only. Microbial life in deep sediments and Earth's crust is now acknowledged by the scientific world. The deep subsurface biosphere contributes significantly to fundamental biogeochemical processes. However, despite great advances in geo-microbiological studies, deep terrestrial ecosystems are microbiologically poorly understood, mainly due to their inaccessibility. The drilling down to the base of the Triassic (1980 meters deep) in the geological formations of the eastern Paris Basin performed by ANDRA (EST433) in 2008 provides us a good opportunity to explore the deep biosphere. We conditioned the samples on the coring site, in as aseptic conditions as possible. In addition to storage at atmospheric pressure, a portion of the four Triassic samples was placed in a 190 bars pressurized chamber to investigate the influence of the conservation pressure factor on the found microflora. In parallel, in order to evaluate a potential bacterial contamination of the cores by the drilling fluids, samples of mud just before each sample drilling were taken and analyzed. The microbial exploration can be divided in two parts: - A cultural approach in different culture media for metabolic groups as methanogens, fermenters and sulphate reducing bacteria to stimulate their growth and to isolate microbial cells still viable. - A molecular approach by direct extraction of genomic DNA from the geological samples to explore a larger biodiversity. The limits are here the difficulties to extract DNA from these low biomass containing rocks. After comparison and optimization of several DNA extraction methods, the bacterial diversity present in rock cores was analyzed using DGGE (Denaturating Gel Gradient Electrophoresis) and cloning. The detailed results of all these investigations will be presented: - Despite all 400 cultural conditions experimented (with various media, salinities, temperatures, conservation pressure, agitation), no viable and

  10. Microbiological stability of homeopathic medicines using purified water as vehicle

    Directory of Open Access Journals (Sweden)

    Virginia Teresa Cegalla

    2011-09-01

    Full Text Available Background: Homeopathic medicines are prepared in homeopathic pharmacies. This leads to freedom of prescription but requires more knowledge of the clinicians to achieve the best results. Preparations made of purified water receive a validity of 24 hours, but there are prescriptions for up to 30 days. This contradiction raises tensions among physicians, pharmacists and patients. Aims: to evaluate the increase in microbiological contamination in homeopathic medicines using purified water as vehicle compared with the microbiological stability of purified water. Contribute to the quality of homeopathic medicine and treatment. Methodology: daily microbiological analysis for one week to assess the growth of heterotrophic bacteria, Pseudomonas, yeasts and molds. The reference used was the USP 32/NF 27 and the Brazilian Pharmacopoeia 5th edition. Results: there was a higher growth of microorganisms on the medicine, compared with purified water. From the 2nd day on, this growth has been beyond the legal limits. Discussion: medicines for oral use are not sterile preparations, but they must remain stable during its shelf life. Our results indicate that contamination occurs from the earliest days of use. This shows the need to change the prescription in relation of the vehicle, to ensure hygiene and avoid potential contamination of the patient. It is necessary to prevent conflict of information between pharmacists and patients, and the contradiction of the doctor's advice, besides the potential risk of responsibility to be attributed to the pharmacy. It is necessary to promote a discussion between pharmacists and clinicians, to spread this information for those that prescribe. Conclusion: there was an increased of microbiological contamination of the medicines dispensed in purified water, which harms the quality of homeopathic medicine and homeopathic treatment.

  11. Avaliação de dietas fornecidas dos 14 aos 42 dias de idade sobre o desempenho e a composição de carcaça de leitões Evaluation of diets fed from 14 to 42 days of age on piglets performance and carcass composition

    Directory of Open Access Journals (Sweden)

    Alessandra Gimenez Mascarenhas

    1999-01-01

    Full Text Available O objetivo deste trabalho foi avaliar dietas fornecidas dos 14 aos 42 dias de idade sobre o desempenho e a composição de carcaça de leitões. Vinte e oito leitegadas mestiças (Landrace × Large White foram alimentadas à vontade com quatro tipos de dietas: duas simples e duas complexas. No desmame, aos 21 dias de idade, quatro animais (dois machos castrados e duas fêmeas, foram transferidos para creches e alojados em gaiolas metálicas, onde continuaram a ser alimentados com rações à vontade. Oitenta e quatro animais, 21 para cada tratamento (sete animais aos 14 dias e 14 animais aos 42 dias de idade, foram abatidos para determinar as taxas de deposição de proteína e gordura na carcaça. Foi usado delineamento experimental de blocos ao acaso, com quatro tratamentos e sete repetições por tratamento. Dos 14 aos 21 dias de idade, o consumo diário de ração (CDR foi insignificante. Após o desmame, os maiores CDR e ganho diário de peso (GDP foram observados em leitões alimentados com as dietas complexa e simples, que tinham maior nível de proteína bruta e lisina (19 e 1,25%, que os alimentados com dietas simples contendo 16 e 0,80% de proteína bruta e lisina, respectivamente. As dietas simples e complexas com os mesmos níveis de proteína bruta, lisina e energia digestível proporcionaram resultados semelhantes de desempenho. A redução do nível de proteína bruta dietético piorou o desempenho dos leitões desmamados aos 21 dias.The objective of this work was to evaluate diets fed from 14 to 42 days of age on piglets performance and carcass composition. Twenty-eight crossbred litters (Landrace x Large White were full fed four types of diets: two simple and two complex. In the weaning time, at 21 days of age, four animals (two barrows and two gilts were transferred to a nursery and allotted to metallic cages, where they continued to be full fed with diets. Eight-four animals, 21 animals from each treatment (seven animals at 14

  12. Níveis de suplemento à base de fubá de milho para novilhos Nelore terminados a pasto na seca: desempenho, características de carcaça e avaliação do pasto Levels of corn meal based supplement in Nellore steers finished on pasture in the dry season: performance, carcass characteristics and pasture evaluation

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo Sverzut Baroni

    2010-01-01

    Full Text Available Objetivou-se com este trabalho avaliar a disponibilidade dos componentes da pastagem, o ganho de peso e as características de carcaça de novilhos Nelore recebendo suplemento na fase de terminação durante o período seco. O experimento foi realizado em pastagem de capim-braquiária (Brachiaria brizantha, cv. Marandu, em seis piquetes de 9 hectares. Utilizaram-se 48 novilhos com 30 meses e peso inicial de 412 ± 16 kg, distribuídos ao acaso em seis lotes de oito animais. Cada lote foi alojado em um piquete e pesado no início e a cada 21 dias, durante 84 dias. Os suplementos foram fornecidos nos níveis de 0,25; 0,5; 1,0; 2,0; e 4,0 kg/animal.dia em comparação a um suplemento controle, com apenas mistura mineral. Os suplementos, à exceção do controle, apresentaram níveis variáveis de proteína bruta (87,4 a 25,0% da matéria seca e 25:25:50, 15:15:70, 10:10:80, 5:5:90 e 2,5:2,5:95 para 0,25; 0,5; 1,0; 2,0; e 4,0 kg/animal.dia, respectivamente, uma vez que a ureia foi usada como controladora do consumo de suplemento. O delineamento foi inteiramente casualizado e os níveis de suplementação foram avaliados por análise de regressão. A proporção de colmo e folha seca aumentou no decorrer dos períodos experimentais, caracterizando déficit hídrico da estação seca. Houve efeito linear crescente dos níveis de suplemento sobre o peso vivo final, o ganho de peso médio diário e o ganho médio por período, tanto em relação ao consumo de suplemento quanto em relação ao consumo de proteína bruta e nutrientes digestíveis totais. Os níveis de suplemento têm efeito linear crescente sobre o peso de carcaça e a espessura de gordura subcutânea e efeito quadrático sobre o rendimento de carcaça.The objective of this study was to evaluate the availability of pasture components, average daily gain and carcass characteristics of Nellore steers receiving supplements in the finishing phase during the dry season. The experiment was

  13. Use of expected progeny differences for marbling in beef: II. Carcass and palatability traits.

    Science.gov (United States)

    Gwartney, B L; Calkins, C R; Rasby, R J; Stock, R A; Vieselmeyer, B A; Gosey, J A

    1996-05-01

    A 2-yr study was conducted to determine the effect of EPD for marbling on marbling score, palatability traits, and carcass fatness in beef. Steer (n = 122) and heifer (n = 123) carcasses were obtained by mating Angus bulls having a high ( > .4) or low ( Simmental, and 1/4 Gelbvieh). Carcass traits, composition of primals, quarters, and sides, palatability, and shear force data were obtained and adjusted to the mean number of days on feed, equal marbling score (Small50), fat thickness (1.0 cm), and carcass weight (318 kg) end points. Steer carcasses from the high marbling EPD group, adjusted to the mean number of days on feed, had significantly more marbling (P carcasses. Heifer carcasses from the high marbling EPD group had better (P carcass without compromising palatability. PMID:8726733

  14. Electromagnetic scanning to estimate carcass lean content of Taiwan native broilers.

    Science.gov (United States)

    Lin, R S; Chen, L R; Huang, S C; Liu, C Y

    2002-07-01

    To estimate lean content of Taiwan native broiler carcasses accurately, objectively and rapidly, electrical conductivity measurements of broiler carcasses and other relative factors (carcass weight, length and temperature) were used in multiple linear regression analysis for lean prediction. Forty native broiler carcasses, with average market weight 2477.5±465.5 g, were scanned through a 10 MHz electromagnetic field created by an electromagnetic scanner (SA-3203) to measure the total body electrical conductivity (TOBEC) index. After scanning, each broiler carcass was separated into wing, breast, leg and back. Each primal cut was dissected into lean, fat and bone. The weight, length, temperature and TOBEC index of broiler carcass were significantly correlated with lean weight of broiler carcass (Pindex showed higher coefficient of determination (R(2)=0.968) and lower coefficient of variation (CV=4.178) with an equation using beheaded carcass weight, temperature and TOBEC index as variables. PMID:22060853

  15. The effect of slaughter operations on the contamination of chicken carcasses with thermotolerant Campylobacter

    DEFF Research Database (Denmark)

    Rosenquist, Hanne; Sommer, Helle Mølgaard; Nielsen, Niels L.;

    2006-01-01

    To evaluate the effect of specific slaughter operations on the contamination of broiler carcasses with naturally occurring thermotolerant Campylobacter, experiments were carried out in two Danish commercial slaughter plants (Plant I and Plant 11). Six broiler flocks determined Campylobacter......) cfu/g, respectively. In packed frozen chickens (Plant II) an additional reduction of 1.38 log(10) cfu/g in average was obtained due to the freezing operation. In packed chilled chickens (Plant 1), however, the number of thermotolerant Campylobacter per gram remained at the same level as after air...

  16. Crude glycerin in diets for wethers in feedlot: intake, digestibility, performance, carcass and meat traits

    OpenAIRE

    Mauriceia Costa Carvalho Barros; Jair de Araújo Marques; Fabiano Ferreira da Silva; Robério Rodrigues Silva; Gilmara Santos Guimarães; Luciano Lemos da Silva; Fabiana Lana de Araújo

    2015-01-01

    The aim was to evaluate the effects of crude glycerin levels (CG) (0, 2.65, 5.33, 8.06 and 10.84% in dry matter basis) on intake, digestibility, performance, carcass morphometric measurements and meat quality of sheep finished in feedlot. Twenty five crossbred Dorper x St. Ines wethers, with 24 ± 2.0 kg average weight were housed in individual pens. The experimental design was completely randomized. Levels of CG in gross diet caused a decreasing linear effect (P

  17. Nutrient digestibility, carcass characteristics and plasma metabolites in kids fed diets supplemented with chromium methionine

    OpenAIRE

    Emami, A.; A. Zali; M. GANJKHANLOU; A. AKBARI AFJANI

    2012-01-01

    This study was carried out to evaluate the effects of different levels of chromium methionine (CrMet) on nutrient digestibility, carcass characteristics and plasma metabolites of male kids. Thirty-two male Mahabadi goat kids (average initial body weight (BW) = 22±2 kg, 4 months) were allocated in a completely randomized design with four treatments: 1) control (without Cr), 2) 0.5, 3) 1 and 4) 1.5 mg Cr as Cr-Met/animal/day. Diets were same (ratio of forage: concentrate was 30:70) except for t...

  18. Growth and carcass characteristics of three Ethiopian indigenous goats fed concentrate at different supplementation levels

    OpenAIRE

    Tadesse, Dereje; Urge, Mengistu; Animut, Getachew; Mekasha, Yoseph

    2016-01-01

    The study was carried out to evaluate the effect of genotypes and concentrate levels on growth performance and carcass characteristics of Bati, Hararghe highland (HH) and Short eared Somali (SS) goat types found in Ethiopia. A 3 × 2 factorial arrangement (3 genotype × 2 concentrate levels) was used to randomly allocate 36 goats (15.2 ± 0.30 kg initial weight); 12 goats from each genotype with age about 1 year were divided randomly into two groups for a feeding trial of 90 days. The two concen...

  19. Growth performance and carcass characteristics of feedlot cattle fed different levels of macadamia oil cake.

    Science.gov (United States)

    Acheampong-Boateng, O; Mikasi, M S; Benyi, K; Amey, A K A

    2008-04-01

    Eighteen cattle (six Bonsmara males, seven Simmanteler x Beefmaster males and five Simmanteler x Beefmaster females) were assigned to three diets containing 0% (Control), 10% and 20% Macadamia oil cake to evaluate the effects of different levels of Macadamia oilcake (MOC) on feed intake, growth performance and carcass characteristics of feedlot cattle. Differences in average feed intake were not significant (P > 0.05). Average daily gains on the 0% and 20% MOC diets were not significantly different (P 0.05). There were no condemned livers, suggesting that either there were no toxic factors in the feed or, even if present, were probably inactive in the liver.

  20. Physiological indicators of stress and meat and carcass characteristics in tail bitten slaughter pigs

    OpenAIRE

    Valros, Anna; Munsterhjelm, Camilla; Puolanne, Eero; Ruusunen, Marita; Heinonen, Mari; Peltoniemi, Olli A T; Pösö, A. Reeta

    2013-01-01

    Background Tail biting is a common welfare problem in pig production and in addition to being a sign of underlying welfare problems, tail biting reduces welfare in itself. The aim of this study was to evaluate the effects of tail biting on different pre and post mortem indicators of stress in slaughter pigs and on carcass and meat characteristics. A total of 12 tail bitten (TB) and 13 control (C) pigs from a farm with a long-term tail biting problem were selected for salivary cortisol analyse...