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Sample records for carbohydrate grain filling

  1. Effect of Different Irrigation Patterns on Transportation and Allocation of Carbohydrate During Grain Filling of Rice

    Institute of Scientific and Technical Information of China (English)

    CHEN Hai-sheng; TAO Long-xing; WANG Xi; HUANG Xiao-lin; TAN Hui-juan; CHENG Shi-hua; MIN Shao-kai

    2005-01-01

    Using intersubspecific hybrid rice Xieyou 9308 and Liangyou Peijiu as the tested materials, the effects of different irrigation patterns on transportation and allocation of carbohydrate during grain filling stage was observed by the design with three level of soil water content in irrigated field. The results showed that in the conventional flooding and the alternate dry-watering cultivations, the exported rate of stored carbohydrate from stem and photophate from the leaves were 60 and 90 % respectively. The exported rate of carbohydrate was decreased significantly (P<0.01) in the non-flooding cultivation. There was no significant difference between the conventional flooding and dry-wet alternation treatments in term of the exported rate of carbohydrate. The filling grains were the major sinks for carbohydrate storage during grain filling stage. Grains received nearly 50% of stored carbohydrate from leaf sheath and 80% of photophate from leaves. At the non-flooding condition the absorbing rates of grains were significantly decreased by 10 % from leaf sheath and 20 %from leaves photophate. Water stress leaded much decrease in absorbing ability for inferior grains, which might be one of the main causes for low seed-settling rate in non-flooding cultivation.

  2. Photosynthesis, Carbohydrate Storage and Remobilization During Grain Filling as Affected by Sulphur Application in Winter Wheat

    Institute of Scientific and Technical Information of China (English)

    WANG Dong; YU Zhen-wen; XU Zhen-zhu

    2005-01-01

    Photosynthesis and carbohydrate storage and remobilization was investigated during grain filling of winter wheat (Triticum aestivum L., cv. Lumai 22) in sulphur application experiments where the available sulphur (S) content in the soil of 0-20 cm soil layer was 5.84 mg kg-1. Two levels of S were applied as S0 (0 kg S ha-1) and S1 (67.5 kg S ha-1). The results showed that net photosynthetic rate of flag leaf of S1 treatment was significantly higher than S0 treatment, and it increased gradually from anthesis to 35 DAA. Sucrose-phosphate synthase (SPS) activity and sucrose content of S1 treatment were significantly higher than those of S0 treatment. The contents of total soluble sugar, sucrose, fructose, glucose, DP3 and DP ≥ 4fructan in stem and sheath of S1 treatment were significantly higher than those of S0 treatment also. The amount of total soluble sugar and fructans remobilization from stem and sheath and the starch accumulating rate in grain of S1 treatment was significantly higher than those of S0 treatment. It is suggested that sulphur application increase photosynthate remobilization from stem and sheath to grain at later filling stage.

  3. Variations in Carbohydrate and Protein Accumulation among Spikelets at Different Positions Within a Panicle During Rice Grain Filling

    Institute of Scientific and Technical Information of China (English)

    DONG Ming-hui; CHEN Pei-feng; XIE Yu-lin; QIAO Zhong-ying; YANG Jian-chang

    2012-01-01

    The accumulation dynamics of kernel components for spikelets at different positions within a rice panicle were investigated during grain filling to understand the physiological reasons for the variation of grain quality.Two rice cultivars,Yangdao 6 (indica) and Yangjing 9538 (japonica),were field-grown,and the grain filling characters and contents of starch,soluble sugar,and protein of the spikelets at different positions were studied.There were significant differences in matter accumulation among spikelets at different positions during grain filling.The early-flowering spikelets presented dominance over the late-flowering spikelets in initial time and initial rate of accumulation.At the initial and mid filling stages,the contents and the rates of starch and amylose accumulation in spikelets decreased with the flowering sequence,but soluble sugar content (SSC) exhibited the opposite trend.The difference in SSC among the spikelets of Yangjing 9538 was greater than that of Yangdao 6,but amylose content in mature spikelets showed no obvious relationship to their flowering sequence.The crude protein content (CPC) of early-flowering spikelets decreased more rapidly than that of late-flowering ones at the initial filling stage,and CPC in the spikelets on the secondary branch was higher than that on the primary branch,but CPC in early-flowering ones was lower than that in late-flowering across the whole grain filling period.Grain water content (GWC) of early-flowering spikelets decreased more rapidly than that of late-flowering spikelets on the same branch at the initial and mid filling stages,especially for the top grain on each primary branch.The results suggested that poor grain filling of late-flowering spikelets may be attributed to their low biological activity rather than carbohydrate supply limitation.

  4. Bulk carbohydrate grain filling of barley ß-glucan mutants studied by 1H HR MAS NMR

    DEFF Research Database (Denmark)

    Seefeldt, Helene Fast; Larsen, Flemming Hofmann; Viereck, Nanna;

    2008-01-01

    ) during grain filling. For the first time, 1H HR MAS NMR spectra of flour from immature barley seeds are analyzed. Spectral assignments are made using two-dimensional (2D) NMR methods. Both α- and β-glucan biosynthesis were characterized by inspection of the spectra as well as by calibration......Temporal and genotypic differences in bulk carbohydrate accumulation in three barley genotypes differing in the content of mixed linkage β-(1→3),(1→4)-D-glucan (β-glucan) and starch were investigated using proton high-resolution, magic angle spinning, nuclear magnetic resonance (1H HR MAS NMR...

  5. Carbohydrates accumulation and remobilization in wheat plants as influenced by combined waterlogging and shading stress during grain filling

    DEFF Research Database (Denmark)

    Li, H.; Cai, J.; Jiang, D.;

    2013-01-01

    Wheat plants were subjected to combined waterlogging and shading stress (WS) at 0–7, 8–15, 16–23 and 24–31 days after anthesis (DAA), respectively. Compared to the non-stressed plants, WS significantly decreased the final grain yield. Grain number was dramatically lowered by WS imposed at 0–7 DAA...

  6. Kinetic Modeling of Sunflower Grain Filling and Fatty Acid Biosynthesis

    Science.gov (United States)

    Durruty, Ignacio; Aguirrezábal, Luis A. N.; Echarte, María M.

    2016-01-01

    Grain growth and oil biosynthesis are complex processes that involve various enzymes placed in different sub-cellular compartments of the grain. In order to understand the mechanisms controlling grain weight and composition, we need mathematical models capable of simulating the dynamic behavior of the main components of the grain during the grain filling stage. In this paper, we present a non-structured mechanistic kinetic model developed for sunflower grains. The model was first calibrated for sunflower hybrid ACA855. The calibrated model was able to predict the theoretical amount of carbohydrate equivalents allocated to the grain, grain growth and the dynamics of the oil and non-oil fraction, while considering maintenance requirements and leaf senescence. Incorporating into the model the serial-parallel nature of fatty acid biosynthesis permitted a good representation of the kinetics of palmitic, stearic, oleic, and linoleic acids production. A sensitivity analysis showed that the relative influence of input parameters changed along grain development. Grain growth was mostly affected by the specific growth parameter (μ′) while fatty acid composition strongly depended on their own maximum specific rate parameters. The model was successfully applied to two additional hybrids (MG2 and DK3820). The proposed model can be the first building block toward the development of a more sophisticated model, capable of predicting the effects of environmental conditions on grain weight and composition, in a comprehensive and quantitative way. PMID:27242809

  7. Association of Phytate Formation with Grain Filling in Rice

    Institute of Scientific and Technical Information of China (English)

    WANG Ruo-zhong; XIAO Lang-tao; DING Jun-hui; YAN Qin-quan

    2003-01-01

    The grain-filling characteristics of six rice varieties (combinations) and the relationships between their relative biochemistry composition during phytate synthesizing and grain plumpness were studied. Regarding results for ISHR1,ISHR2, R198 and JW21, with good grain plumpness, the two-step-filling in superior spikelets and inferior spikelets was not clear, while for ISHR3 and 559, with poor grain plumpness, it was very clear. From booting stage to flowering stage, the contents of phytate and inositol in varieties with good grain plumpness was obviously higher than those in varieties with poor grain plumpness. While at grain filling stage, the content of inorganic phosphorus in varieties with poor grain plumpness was obviously higher than that in varieties with good grain plumpness. The contents of phytate and inositol from booting stage to flowering stage was highly significantly correlated with the initial filling power (RO), the mean filling rate (RM) and grain filling percentage (PGF), and the content of inorganic phosphorus at grain filling stage was negatively significantly correlated with R0, FM and PGF. Furthermore, effective approach to improving grain filling was put forward.

  8. Protease-induced solubilisation of carbohydrates from brewers' spent grain

    NARCIS (Netherlands)

    Faulds, C.B.; Collins, S.; Robertson, J.A.; Treimo, J.; Eijsink, V.G.H.; Hinz, S.W.A.; Schols, H.A.; Buchert, J.; Waldron, K.W.

    2009-01-01

    The impact of microbial proteases on the release of carbohydrates from BSG was studied. The proteases were able to release the non-cellulosic glucose, a portion of feruloylated arabinoxylan and over 50% of the protein from brewers' spent grain (BSG) after 24 h hydrolysis. The non-cellulosic glucose

  9. Hydrolysis of Brewers' Spent Grain by Carbohydrate Degrading Enzymes

    NARCIS (Netherlands)

    Forssell, P.; Kontkanen, H.; Schols, H.A.; Hinz, S.W.A.; Eijsink, V.G.H.; Treimo, J.; Robertson, J.A.; Waldron, K.W.; Faulds, C.B.; Buchert, J.

    2008-01-01

    In this work four commercial cellulase-hemicellulase mixtures with different activity profiles were used for solubilization of carbohydrates from brewers' spent grain (BSG). After the enzyme treatment, both the solubilised fraction and the unhydrolysed residue were characterized. Treatment with 5,00

  10. High Temperature at Grain-filling Stage Affects Nitrogen Metabolism Enzyme Activities in Grains and Grain Nutritional Quality in Rice

    Institute of Scientific and Technical Information of China (English)

    LIANG Cheng-gang; CHEN Li-ping; WANG Yan; LIU Jia; Xu Guang-li; LI Tian

    2011-01-01

    Rice plants would more frequently suffer from high temperature (HT) stress at the grain-filling stage in future.A japonica rice variety Koshihikari and an indica rice variety IR72 were used to study the effect of high temperature on dynamic changes of glutamine synthetase (GS) activity,glutamate synthase (GOGAT) activity,glutamic oxalo-acetic transminase (GOT) activity,glutamate pyruvate transminase (GPT) activity in grains and grain nutritional quality at the grain-filling stage.Under HT,the activities of GOGAT,GOT,GPT and soluble protein content in grains significantly increased,whereas GS activity significantly decreased at the grain-filling stage.In addition to the increase of protein and amino acids contents,it was suggested that GOGAT,GOT and GPT in grains played important roles in nitrogen metabolism at the grain-filling stage.Since the decrease of GS activity in grains did not influence the accumulations of amino acids and protein,it is implied that GS might not be the key enzyme in regulating glutamine content in grains.

  11. Changes in Activities of the Key Enzymes Related to Starch Synthesis in Rice Grains During Grain Filling and Their Relationships with the Filling Rate and Cooking Quality

    Institute of Scientific and Technical Information of China (English)

    ZHAO Bu-hong; ZHANG Wen-jie; WANG Zhi-qin; ZHU Qin-sen; YANG Jian-chang

    2005-01-01

    With 10 rice cultivars (lines) as materials, the changes in activities of adenosine diphosphoglucose pyrophosphorylase (ADPGPase), starch synthase (SSase) and starch branching enzyme (Q-enzyme) in the grains during grain filling, and their relationships with the filling rate, gel consistency (GC), alkali spreading value (ASV) and amylose content (AC) were studied. The results showed that changes in activities of ADPGPase, SSase and Q-enzyme exhibited a single peak during grain filling, and the time of the activity peaks for the former two enzymes was earlier than that of the maximum grain-filling rate (Tmax), and the time reaching the peak for Q-enzyme was synchronous with Tmax. The activities at early grain filling stage, and the mean and maximum activities of each enzyme during grain filling period were positively and significantly or very significantly correlated with the mean and maximum grain filling rate and starch content (mg grain-1) in the grains.Activities of ADPGPase at all grain filling stages and those of Q-enzyme at the early and mid filling stages were not significantly correlated the cooking quality (GC, ASV and AC). SSase activities at the early filling stage were significantly and negatively correlated with GC and ASV, and positively correlated with AC. Activities of SSase at mid and late grain filling stages and Q-enzyme at the late filling stage were significantly and positively correlated with GC and ASV, and negatively correlated with AC. Spraying zeatin or abscisic acid at early grain filling stage could obviously regulate the activities of ADPGPase, SSase and Q-enzyme in the grains.

  12. Proteome analysis of grain filling and seed maturation in barley.

    Science.gov (United States)

    Finnie, Christine; Melchior, Sabrina; Roepstorff, Peter; Svensson, Birte

    2002-07-01

    In monocotyledonous plants, the process of seed development involves the deposition of reserves in the starchy endosperm and development of the embryo and aleurone layer. The final stages of seed development are accompanied by an increase in desiccation tolerance and drying out of the mature seed. We have used two-dimensional gel electrophoresis for a time-resolved study of the changes in proteins that occur during seed development in barley (Hordeum vulgare). About 1,000 low-salt extractable protein spots could be resolved on the two-dimensional gels. Protein spots were divided into six categories according to the timing of appearance or disappearance during the 5-week period of comparison. Nineteen different proteins or protein fragments in 36 selected spots were identified by matrix-assisted laser-desorption ionization time of flight mass spectrometry (MS) or nano-electrospray tandem MS/MS. Some proteins were present throughout development (for example, cytosolic malate dehydrogenase), whereas others were associated with the early grain filling (ascorbate peroxidase) or desiccation (Cor14b) stages. Most noticeably, the development process is characterized by an accumulation of low-M(r) alpha-amylase/trypsin inhibitors, serine protease inhibitors, and enzymes involved in protection against oxidative stress. We present examples of proteins not previously experimentally observed, differential extractability of thiol-bound proteins, and possible allele-specific spot variation. Our results both confirm and expand on knowledge gained from previous analyses of individual proteins involved in grain filling and maturation.

  13. Effect of removing superior spikelets on grain filling of inferior spikelets in rice

    Directory of Open Access Journals (Sweden)

    Cuicui You

    2016-08-01

    Full Text Available Abstract: Large-panicle rice cultivars often fail to reach their yield potential due to the poor grain filling of inferior spikelets (IS. Thus, it is important to determine the causes of poor IS grain filling. In this study, we attempted to identify whether inferior grain filling of large panicles is restricted by superior spikelets (SS and their physiological mechanism. SS were removed from two homozygous japonica rice strains (W1844 and WJ165 during flowering in an attempt to force photosynthate transport to the IS. We measured the effects of SS removal on seed setting rate, grain weight, grain filling rate, sucrose content, as well as hormone levels, activities of key enzymes, and expression of genes involved in sucrose to starch metabolism in rice IS during grain filling. The results showed that SS removal improved IS grain filling by increasing the seed setting rate, grain weight, sucrose content, and hormone levels. SS removal also enhanced the activities of key enzymes and the expression levels of genes involved in sucrose to starch metabolism. These results suggest that sucrose and several hormones act as signal substances and play a vital role in grain filling by regulating enzyme activities and gene expression. Therefore, IS grain filling is restricted by SS, which limit assimilate supply and plant hormones, leading to poor grain filling of IS.

  14. The relationship between polyamines and hormones in the regulation of wheat grain filling.

    Directory of Open Access Journals (Sweden)

    Yang Liu

    Full Text Available The grain weight of wheat is strongly influenced by filling. Polyamines (PA are involved in regulating plant growth. However, the effects of PA on wheat grain filling and its mechanism of action are unclear. The objective of the present study was to investigate the relationship between PAs and hormones in the regulation of wheat grain filling. Three PAs, spermidine (Spd, spermine (Spm, and putrescine (Put, were exogenously applied, and the grain filling characteristics and changes in endogenous PA and hormones, i.e., indole-3-acetic acid (IAA, zeatin (Z + zeatin riboside (ZR, abscisic acid (ABA, ethylene (ETH and gibberellin 1+4 (GAs, were quantified during wheat grain filling. Exogenous applications of Spd and Spm significantly increased the grain filling rate and weight, but exogenous Put had no significant effects on these measures. Exogenous Spd and Spm significantly increased the endogenous Spd, Spm, Z+ZR, ABA, and IAA contents and significantly decreased ETH evolution in grains. The endogenous Spd, Spm and Z+ZR contents were positively and significantly correlated with the grain filling rate and weight of wheat, and the endogenous ETH evolution was negatively and significantly correlated with the wheat grain filling rate and weight. Based upon these results, we concluded that PAs were involved in the balance of hormones that regulated the grain filling of wheat.

  15. Testing the responses of four wheat crop models to heat stress at anthesis and grain filling.

    Science.gov (United States)

    Liu, Bing; Asseng, Senthold; Liu, Leilei; Tang, Liang; Cao, Weixing; Zhu, Yan

    2016-05-01

    Higher temperatures caused by future climate change will bring more frequent heat stress events and pose an increasing risk to global wheat production. Crop models have been widely used to simulate future crop productivity but are rarely tested with observed heat stress experimental datasets. Four wheat models (DSSAT-CERES-Wheat, DSSAT-Nwheat, APSIM-Wheat, and WheatGrow) were evaluated with 4 years of environment-controlled phytotron experimental datasets with two wheat cultivars under heat stress at anthesis and grain filling stages. Heat stress at anthesis reduced observed grain numbers per unit area and individual grain size, while heat stress during grain filling mainly decreased the size of the individual grains. The observed impact of heat stress on grain filling duration, total aboveground biomass, grain yield, and grain protein concentration (GPC) varied depending on cultivar and accumulated heat stress. For every unit increase of heat degree days (HDD, degree days over 30 °C), grain filling duration was reduced by 0.30-0.60%, total aboveground biomass was reduced by 0.37-0.43%, and grain yield was reduced by 1.0-1.6%, but GPC was increased by 0.50% for cv Yangmai16 and 0.80% for cv Xumai30. The tested crop simulation models could reproduce some of the observed reductions in grain filling duration, final total aboveground biomass, and grain yield, as well as the observed increase in GPC due to heat stress. Most of the crop models tended to reproduce heat stress impacts better during grain filling than at anthesis. Some of the tested models require improvements in the response to heat stress during grain filling, but all models need improvements in simulating heat stress effects on grain set during anthesis. The observed significant genetic variability in the response of wheat to heat stress needs to be considered through cultivar parameters in future simulation studies.

  16. Improving cereal grain carbohydrates for diet and health.

    Science.gov (United States)

    Lafiandra, Domenico; Riccardi, Gabriele; Shewry, Peter R

    2014-05-01

    Starch and cell wall polysaccharides (dietary fibre) of cereal grains contribute to the health benefits associated with the consumption of whole grain cereal products, including reduced risk of obesity, type 2 diabetes, cardiovascular disease and colorectal cancer. The physiological bases for these effects are reviewed in relation to the structures and physical properties of the polysaccharides and their behaviour (including digestion and fermentation) in the gastro-intestinal tract. Strategies for modifying the content and composition of grain polysaccharides to increase their health benefits are discussed, including exploiting natural variation and using mutagenesis and transgenesis to generate further variation. These studies will facilitate the development of new types of cereals and cereal products to face the major health challenges of the 21st century.

  17. Dry matter and water dynamics of wheat grains in response to source reduction at different phases of grain filling

    Directory of Open Access Journals (Sweden)

    Claudia I Harcha

    2014-12-01

    Full Text Available Agreement that wheat (Triticum aestivum L. is scarcely limited by source of assimilates during grain fill has not been confirmed when source was drastically decrease in high yield potential environments. Dry matter (DM and water dynamics of growing grains being possibly able to explain grain weight (GW responses to source-sink ratios (S-Sratios in these conditions. Objectives were to evaluate response of GW to S-Sratios at different phases during grain fill, and relationship between DM and grain water content in response to different S-Sratios. Wheat was sown at field conditions during 20042005 and 2005-2006 growing seasons. Four S-Sratios were assessed: Control without S-Sratios modification (C, S-Sratios from anthesis (At +12 d to physiological maturity (ShAll, S-Sratios from At +12 d to first half of grain fill (Sh1st and from second half of grain fill to maturity (Sh2nd. Thousand grain weight (TGW, GW at individual positions (IGW, stabilized grain water content (SGWC and grain filling rate (GFR were measured. TGW sensitivity to S-Sratios varied according to length of treatment and its timing, i.e. ShAll, Sh1st, and Sh2nd treatments reduced TGW by 48%, 26%, and 22%, respectively. These reductions were little higher when IGW were evaluated in ShAll (i.e. 53% and Sh1st (i.e. 33% treatments and lower in Sh2nd (i.e. 12%. SGWC sensitivity was lower than that of IGW across S-Sratios (e.g. SIiau 27%, Shust 22%, and Sh2nd 5%. However, close association between IGW and SGWC (R² = 0.78, p < 0.001 and between GFR and SGWC (R² = 0.98, p ≤ 0.001 was found regardless of S-Sratios and seasons

  18. Carbohydrates

    Science.gov (United States)

    Carbohydrates are one of the main types of nutrients. They are the most important source of energy for your body. Your digestive system changes carbohydrates into glucose (blood sugar). Your body uses this ...

  19. Changes in Activities of Glutamine Synthetase during Grain Filling and Their Relation to Rice Quality

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Four japonica rice varieties differed in cooking and eating qualities were used in a pot experiment to study the relationship between the activities of glutamine synthetase during grain filling and rice quality. The activities of glutamine synthetase gradually increased and then declined as a single peak curve in the course of grain filling. The 15th day after heading was a turning point, before which the enzymatic activities in the inferior rice varieties with high protein content were higher than those in the superior rice varietie with low protein content, and after which it was converse. The activity of glutamine synthetase in grain was correlated with the taste meter value, peak viscosity and breakdown negatively at the early stage of grain filling whereas positively at the middle and late stages. Moreover, it was correlated with the protein content of rice grain and setback positively at the early stage and negatively at the middle and late stages. The correlation degree varied with the course of grain filling. From 15 days to 20 days after heading was a critical stage, in which the direction of correlation between the activity of glutamine synthetase and taste meter value and RVA properties of rice changed.

  20. Natural leaf senescence: probed by chlorophyll fluorescence, CO2 photosynthetic rate and antioxidant enzyme activities during grain filling in different rice cultivars.

    Science.gov (United States)

    Panda, Debabrata; Sarkar, Ramani Kumar

    2013-01-01

    Natural leaf senescence was investigated in four rainfed lowland rice cultivars, FR 13A (tolerant to submergence), Sabita and Sarala (adapted to medium depth, 0-50 cm stagnant flooding) and Dengi (conventional farmers' cultivar). Changes in the levels of pigment content, CO2 photosynthetic rate, photosystem II photochemistry and anti-oxidant enzyme activities of flag leaves during grain-filling stage were investigated. Chlorophyll content, photochemical efficiency of photosystem II and CO2 photosynthetic rate decreased significantly with the progress of grain-filling. Likely, the activities of antioxidant enzymes namely, superoxide dismutase, catalase, guaiacol peroxidase and ascorbate peroxidase decreased with progress of grain-filling. A substantial difference was observed among the four cultivars for the sustainability index (SI) of different photosynthetic parameters and antioxidant enzyme activities; SIs of those parameters, in general, were lower in low yielding cultivar FR 13A compared to the other three cultivars. Among the four cultivars Sabita gave maximum grain yield. Yet, SI of Pn was greater in Sarala and Dengi compared to the Sabita. SIs of electron transport (ETo/CS), maximal photochemical efficiency (Fv/Fm), area above Fo and Fm, catalase and ascorbate peroxidase were also greater in Sarala and Dengi. The data showed that among the different Chl a fluorescence parameters, PI could be used with greater accuracy to distinguish slow and fast senescence rice cultivars during grain-filling period. It was concluded that maintaining the vitality of rice plants during grain-filling gave guarantee to synthesize carbohydrate, however greater yield could be realized provided superior yield attributing parameters are present.

  1. Compositional Analysis of Whole Grains, Processed Grains, Grain Co-Products, and Other Carbohydrate Sources with Applicability to Pet Animal Nutrition.

    Science.gov (United States)

    Beloshapka, Alison N; Buff, Preston R; Fahey, George C; Swanson, Kelly S

    2016-03-25

    Our objective was to measure the proximate, starch, amino acid, and mineral compositions of grains, grain co-products, and other carbohydrate sources with potential use in pet foods. Thirty-two samples from barley (barley flake, cut barley, ground pearled barley, malted barley, whole pearled barley, pearled barley flakes, and steamed rolled barley); oats (groats, ground oatmeal, ground steamed groats, instant oats, oat bran, oat fiber, oat flour, quick oats, regular rolled oats, steamed rolled oat groats, and steel cut groats); rice (brown rice, polished rice, defatted rice bran, and rice flour); and miscellaneous carbohydrate sources (canary grass seed, hulled millet, whole millet, quinoa, organic spelt hull pellets, potato flake, sorghum, whole wheat, and whole yellow corn) were analyzed. Crude protein, amino acid, fat, dietary fiber, resistant starch, and mineral concentrations were highly variable among the respective fractions (i.e., barley flake vs. malted barley vs. steamed rolled barley) as well as among the various grains (i.e., barley flake vs. brown rice vs. canary grass seed). These ingredients not only provide a readily available energy source, but also a source of dietary fiber, resistant starch, essential amino acids, and macrominerals for pet diets.

  2. Grain filling, starch degradation and feeding value of maize for ruminants

    NARCIS (Netherlands)

    Ali, M.

    2013-01-01

    Keywords; Maize (Zea mays L), Genotypes, Grain filling, Growth temperature, Kernels, Gas production, Starch degradation, Oven-drying, Silage, Ensiling temperature, Ensiling duration, Feeding value, Lactating cows Maize (Zea mays L.) is a major component in the ration of dairy cows in many parts of

  3. Iminosugar inhibitors of carbohydrate-active enzymes that underpin cereal grain germination and endosperm metabolism

    DEFF Research Database (Denmark)

    Andriotis, Vasilios M. E.; Rejzek, Martin; Rugen, Michael D.;

    2016-01-01

    limited knowledge about the nature and control of starch degradation in plants. Increased societal and commercial demand for enhanced yield and quality in starch crops requires a better understanding of starch metabolism as a whole. Here we review recent advances in understanding the roles of carbohydrate......-active enzymes in starch degradation in cereal grains through complementary chemical and molecular genetics. These approaches have allowed us to start dissecting aspects of starch degradation and the interplay with cell-wall polysaccharide hydrolysis during germination. With a view to improving and diversifying...... the properties and uses of cereal grains, it is possible that starch degradation may be amenable to manipulation through genetic or chemical intervention at the level of cell wall metabolism, rather than simply in the starch degradation pathway per se....

  4. Dynamics of a Grain-Filled Ball on a Vibrating Plate

    Science.gov (United States)

    Pacheco-Vázquez, F.; Ludewig, F.; Dorbolo, S.

    2014-09-01

    We study experimentally how the bouncing dynamics of a hollow ball on a vibrating plate is modified when it is partially filled with liquid or grains. Whereas empty and liquid-filled balls display a dominant chaotic dynamics, a ball with grains exhibits a rich variety of stationary states, determined by the grain size and filling volume. In the collisional regime, i.e., when the energy injected to the system is mainly dissipated by interparticle collisions, an unexpected period-1 orbit appears independently of the vibration conditions, over a wide range. This is a self-regulated state driven by the formation and collapse of a granular gas within the ball during one cycle. In the frictional regime (dissipation dominated by friction), the grains move collectively and generate different patterns and steady modes: oscillons, waves, period doubling, etc. From a phase diagram and a geometrical analysis, we deduce that these modes are the result of a coupling (synchronization) between the vibrating plate frequency and the trajectory followed by the particles inside the cavity.

  5. Carbohydrates

    Science.gov (United States)

    ... products, and do not help you feel as satisfied. FIBER High-fiber foods include: Whole grains, such ... ADAM Health Solutions. About MedlinePlus Site Map FAQs Customer Support Get email updates Subscribe to RSS Follow ...

  6. Effects of high NH4+ on K+ uptake, culm mechanical strength and grain filling in wheat

    Directory of Open Access Journals (Sweden)

    Lingan eKong

    2014-12-01

    Full Text Available It is well established that a high external NH4+ concentration depresses many processes in plant development, but the underlying mechanisms are still not well understood. To determine whether the negative effects of high levels of NH4+ are related to competitive cation uptake, wheat was grown in a field with moderate (18 g N m-2 and high (30 g N m-2 supplies of NH4+ in the presence or absence of additional K+ (6 g K2O m-2 to examine culm mechanical strength, the main components of the vascular bundle, nitrogen (N remobilization and the grain-filling rate. The results indicated that an excessive supply of NH4+ significantly decreased culm mechanical strength, the cellulose and lignin contents of vascular bundles, the N remobilization efficiency (NRE and the grain-filling rate compared with a moderate level of NH4+. The additional provision of K+ considerably alleviated these negative effects of high NH4+, resulting in a 19.41%-26.95% increase in culm mechanical strength during grain filling and a 34.59% increase in the NRE. An assay using the scanning ion-selective electrode technique (SIET showed that the net rate of transmembrane K+ influx decreased by 84.62%, and measurements using flame photometry demonstrated that the K+ content decreased by 36.13% in wheat plants subjected to high NH4+. This study indicates that the effects of high NH4+ on culm mechanical strength, cellulose and lignin contents, the NRE and the grain-filling rate are probably associated with inhibition of K+ uptake in wheat.

  7. Changes in Activities of Key Enzymes for Starch Synthesis and Glutamine Synthetase in Grains of Progenies from a Rice Cross During Grain Filling

    Institute of Scientific and Technical Information of China (English)

    LI Xiao-guang; LIU Hai-ying; JIN Zheng-xun; LIU Hong-liang; HUANG Xing; XU Mei-lan; ZHANG Feng-zhuan

    2010-01-01

    The progenies differed in amylose and protein contents in grains, which derived from a rice cross, Dongnong 423×Toukei 180, were used to study changes in the activities of ADP-glucose pyrophosphorylase (AGPP), soluble starch synthetase (SSS), starch branching enzyme (SBE) and glutamine synthetase (GS) in rice grains during grain filling. The activities of AGPP, SSS and SBE gradually increased and then declined as a single-peak curve with the process of grain filling in the progenies with high and low amylose contents in grains. The progenies with high amylose content peaked earlier in the AGPP, SSS and SBE activities and had higher AGPP, SSS and SBE activities at the early grain filling stage than those with low amylose content. The GS activity peaked earlier and was higher at the late stage of grain filling in the progenies with high protein content than in those with low protein content. It is suggested that the activities of key enzymes for starch synthesis and glutamine synthetase could be changed in oriented breeding for amylose and protein contents in grains.

  8. Serine carboxypeptidase 46 Regulates Grain Filling and Seed Germination in Rice (Oryza sativa L.)

    Science.gov (United States)

    Li, Zhiyong; Tang, Liqun; Qiu, Jiehua; Zhang, Wen; Wang, Yifeng; Tong, Xiaohong; Wei, Xiangjin; Hou, Yuxuan

    2016-01-01

    Serine carboxypeptidase (SCP) is one of the largest groups of enzymes catalyzing proteolysis for functional protein maturation. To date, little is known about the function of SCPs in rice. In this study, we present a comprehensive analysis of the gene structure and expression profile of 59 rice SCPs. SCP46 is dominantly expressed in developing seeds, particularly in embryo, endosperm and aleurone layers, and could be induced by ABA. Functional characterization revealed that knock-down of SCP46 resulted in smaller grain size and enhanced seed germination. Furthermore, scp46 seed germination became less sensitive to the ABA inhibition than the Wild-type did; suggesting SCP46 is involved in ABA signaling. As indicated by RNA-seq and qRT-PCR analysis, numerous grain filling and seed dormancy related genes, such as SP, VP1 and AGPs were down-regulated in scp46. Yeast-two-hybrid assay also showed that SCP46 interacts with another ABA-inducible protein DI19-1. Taken together, we suggested that SCP46 is a master regulator of grain filling and seed germination, possibly via participating in the ABA signaling. The results of this study shed novel light into the roles of SCPs in rice. PMID:27448032

  9. Serine carboxypeptidase 46 Regulates Grain Filling and Seed Germination in Rice (Oryza sativa L..

    Directory of Open Access Journals (Sweden)

    Zhiyong Li

    Full Text Available Serine carboxypeptidase (SCP is one of the largest groups of enzymes catalyzing proteolysis for functional protein maturation. To date, little is known about the function of SCPs in rice. In this study, we present a comprehensive analysis of the gene structure and expression profile of 59 rice SCPs. SCP46 is dominantly expressed in developing seeds, particularly in embryo, endosperm and aleurone layers, and could be induced by ABA. Functional characterization revealed that knock-down of SCP46 resulted in smaller grain size and enhanced seed germination. Furthermore, scp46 seed germination became less sensitive to the ABA inhibition than the Wild-type did; suggesting SCP46 is involved in ABA signaling. As indicated by RNA-seq and qRT-PCR analysis, numerous grain filling and seed dormancy related genes, such as SP, VP1 and AGPs were down-regulated in scp46. Yeast-two-hybrid assay also showed that SCP46 interacts with another ABA-inducible protein DI19-1. Taken together, we suggested that SCP46 is a master regulator of grain filling and seed germination, possibly via participating in the ABA signaling. The results of this study shed novel light into the roles of SCPs in rice.

  10. Effect of Temperature at Grain Filling Stage on Activities of Key Enzymes Related to Starch Synthesis and Grain Quality of Rice

    Institute of Scientific and Technical Information of China (English)

    JIN Zheng-xun; QIAN Chun-rong; YANG Jing; LIU Hai-ying; JIN Xue-yong

    2005-01-01

    Three japonica rice varieties with different cooking and eating quality were grown at high temperature in the greenhouse and natural field. Effects of temperature at the grain filling stage on these varieties were investigated in terms of the activities of key enzymes related to starch synthesis and cooking and eating quality of rice grain. The high temperature at the grain filling stage increased protein content, and decreased amylose content and taste meter value of rice; inferior grain quality varieties showed a greater magnitude of the increase or decrease than the superior ones. Reaction of rapid visco analyser profiles to the temperature varied with rice varieties. The activities of adenosine diphosphoglucose pyrophosphorylase (AGPP), soluble starch synthase (SSS)and starch branching enzyme (SBE) gradually increased to a peak value, and thereafter declined as grain filling progressed. Enzyme activities in different varieties differed in a same filling stage, and also in the time when the enzyme activity reached a maximum.AGPP and SSS were insensitive to the environmental temperature, but SBE was comparatively sensitive to the temperature, and its activity declined when temperature was too high or too low.

  11. Effects of heat stress during grain filling on the structure and thermal properties of waxy maize starch.

    Science.gov (United States)

    Lu, Dalei; Shen, Xin; Cai, Xuemei; Yan, Fabao; Lu, Weiping; Shi, Yong-Cheng

    2014-01-15

    Clarifying the waxy maize starch physicochemical characteristics response to heat stress could modify starch quality. The effects of heat stress during grain filling (1-40day after pollination) on starch structure and thermal properties of four waxy maize varieties were investigated. The mean day/night temperature during grain filling for heat stress and control treatments was 35.2/16.1°C and 27.4/15.6°C, respectively. Heat stress during grain filling increased the starch average granule size and the proportion of long chains in amylopectin. Starch granules under heat stress presented more pitting or uneven surfaces. X-ray peak intensities in response to heat stress were variety dependent. Heat stress during grain filling decreased the swelling power and increased the gelatinization temperature and retrogradation percentage, while the gelatinization enthalpy was not affected. In conclusion, heat stress during grain filling significantly affected structural characteristics of waxy maize starch and consequently, changed its swelling and thermal properties.

  12. Influence of High Temperature Stress on Net Photosynthesis, Dry Matter Partitioning and Rice Grain Yield at Flowering and Grain Filling Stages

    Institute of Scientific and Technical Information of China (English)

    L Guo-hua; WU Yong-feng; BAI Wen-bo; MA Bao; WANG Chun-yan; SONG Ji-qing

    2013-01-01

    Climate change is recognized to increase the frequency and severity of extreme temperature events. At flowering and grain filling stages, risk of high temperature stress (HTS) on rice might increase, and lead to declining grain yields. A regulated cabinet experiment was carried out to investigate effects of high temperature stress on rice growth at flowering and grain-filling stages. Results showed that no obvious decrease pattern in net photosynthesis appeared along with the temperature rising, but the dry matter allocation in leaf, leaf sheath, culm, and panicle all changed. Dry weight of panicle decreased, and ratio of straw to total above ground crop dry weight increased 6-34%from CK, which might have great effects on carbon cycling and green house gas emission. Grain yield decreased significantly across all treatments on average from 15 to 73%. Occurrence of HTS at flowering stage showed more serious influence on grain yield than at grain filling stage. High temperature stress showed negative effects on harvest index. It might be helpful to provide valuable information for crop simulation models to capture the effects of high temperature stress on rice, and evaluate the high temperature risk.

  13. Structural and histochemical studies on grain-filling in the caryopsis of rice (Oryza sativa L.)

    Indian Academy of Sciences (India)

    S Krishnan; P Dayanandan

    2003-06-01

    The endosperm and embryo that constitute the filial tissues of rice caryopsis are isolated from the maternal tissues by the absence of any symplastic continuity. Nutrients are transported to the endosperm through a single ovular vascular trace present on the ventral side of the ovary. Initially solute enters through the chalaza into the nucellar projection and then into the endosperm. At later stages transport occurs through the nucellar epidermis, centripetally towards the endosperm. The cell walls of the nucellar epidermis are provided with rib-like thickenings. A comparison of grain-filling in C3 and C4 cereals suggests that rice has structural features allied to C3 cereals, such as wheat, but with significant differences.

  14. Cell-wall Invertases from Rice are Differentially Expressed in Caryopsis during the Grain Filling Stage

    Institute of Scientific and Technical Information of China (English)

    Yong-Qin Wang; Gui-Fang Xiao; Zhen Zhu; Xiao-Li Wei; Hong-Lin Xu; Cheng-Lin Chai; Kun Meng; Hong-Li Zhai; Ai-Jun Sun; Yong-Gang Peng; Bin Wu

    2008-01-01

    Cell-wall Invertase plays an important role in sucrose partitioning between source and sink organs in higher plants. To Investigate the role of cell-wall invertases for seed development In rice (Oryza sativa L.), cDNAs of three putative cell-wall invertase genes OsCIN1, OsCIN2 and OsCIN3 were Isolated. Semi-quantitative reverse transcdption-polymerase chain reaction analysis revealed different expression patterns of the three genes in various rice tissues/organs. In developing ceryopses, they exhibited similar temporal expression patterns, expressed highly at the early and middle grain filling stages and gradually declined to low levels afterward. However, the spatial expression patterns of them were very different, with OsCIN1 primarily expressed in the ceryopsis coat, OsCIN2 in embryo and endosperm, and OsCIN3 In embryo. Further RNA in situ hybridization analysis revealed that a strong signal of OsCIN2 mRNA was detected In the vascular parenchyma surrounding the xylem of the chalazal vein and the aleurone layer, whereas OsCIN3 transcdpt was strongly detected in the vascular parenchyma surrounding the phloem of the chalazal vein, cross.cells, the aleurone layer and the nucellar tissue.These data indicate that the three cell-wall invertase genes play complementary/synergetic roles in assimilate unloading during the grain filling stage. In addition, the cell type-specific expression patterns of OsClN3 In source leaf blades end anthers were also Investigated, and its corresponding physiological roles were discussed.

  15. Accumulation of mixed linkage (1¿3) (1¿4)-ß-D-glucan during grain filling in barley

    DEFF Research Database (Denmark)

    Seefeldt, Helene Fast; Blennow, Per Gunnar Andreas; Jespersen, Birthe P Møller

    2009-01-01

    The accumulation of mixed linkage barley (1 → 3) (1 → 4)-β-d-glucan (BG) during grain filling at eight stages was studied using standard reference methods and infrared spectroscopy. Two mutant barley genotypes having higher (starch mutant lys5f) and lower (high lysine mutant lys3a) BG content tha...

  16. Steam explosion of Brewer's spent grain improves enzymatic digestibility of carbohydrates and affects solubility and stability of proteins.

    Science.gov (United States)

    Kemppainen, K; Rommi, K; Holopainen, U; Kruus, K

    2016-09-01

    Steam explosion was studied as a means to improve the enzymatic digestibility of carbohydrates in Brewer's spent grain, a protein and lipid-rich lignocellulosic by-product of the brewing industry. Having temperature, treatment time and the presence of acid catalyst as variables, a treatment at 200 °C for 10 min without an acid catalyst was found to be the most efficient, dissolving 12.1 % of the dry matter. Mainly oligomeric non-cellulosic glucan and arabinoxylan were dissolved, and the remaining insoluble carbohydrates could be efficiently hydrolysed by an enzyme cocktail (75 % hydrolysis yield). The process also caused partial protein degradation and dissolved over a third of the total nitrogen. Meanwhile, the insoluble protein appeared to become more strongly associated with acid-insoluble lignin. Compositional changes observed in the proteins and carbohydrates were supported by the results of epifluorescence microscopy. The process yielded three chemically different fractions which could serve as biorefinery products or intermediates.

  17. Dynamic proteomic analysis reveals a switch between central carbon metabolism and alcoholic fermentation in rice filling grains.

    Science.gov (United States)

    Xu, Sheng Bao; Li, Tang; Deng, Zhu Yun; Chong, Kang; Xue, Yongbiao; Wang, Tai

    2008-10-01

    Accumulation of reserve materials in filling grains involves the coordination of different metabolic and cellular processes, and understanding the molecular mechanisms underlying the interconnections remains a major challenge for proteomics. Rice (Oryza sativa) is an excellent model for studying grain filling because of its importance as a staple food and the available genome sequence database. Our observations showed that embryo differentiation and endosperm cellularization in developing rice seeds were completed approximately 6 d after flowering (DAF); thereafter, the immature seeds mainly underwent cell enlargement and reached the size of mature seeds at 12 DAF. Grain filling began at 6 DAF and lasted until 20 DAF. Dynamic proteomic analyses revealed 396 protein spots differentially expressed throughout eight sequential developmental stages from 6 to 20 DAF and determined 345 identities. These proteins were involved in different cellular and metabolic processes with a prominently functional skew toward metabolism (45%) and protein synthesis/destination (20%). Expression analyses of protein groups associated with different functional categories/subcategories showed that substantially up-regulated proteins were involved in starch synthesis and alcoholic fermentation, whereas the down-regulated proteins in the process were involved in central carbon metabolism and most of the other functional categories/subcategories such as cell growth/division, protein synthesis, proteolysis, and signal transduction. The coordinated changes were consistent with the transition from cell growth and differentiation to starch synthesis and clearly indicated that a switch from central carbon metabolism to alcoholic fermentation may be important for starch synthesis and accumulation in the developmental process.

  18. [Influence of drought on leaf photosynthetic capacity and root growth of soybeans at grain filling stage].

    Science.gov (United States)

    Guo, Shu-jin; Yang, Kai-min; Huo, Jin; Zhou, Yong-hang; Wang, Yan-ping; Li, Gui-quan

    2015-05-01

    A drought-resistant soybean cultivar Jinda 70 and a drought-sensitive soybean cultivar Jindou 26 were taken as test materials. At the grain filling stage, the cultivars were subject to three water treatments including sufficient water supply, light drought stress, and severe drought stress by using pot experiments for research on influence of drought on leaf photosynthetic capacity and root growth of soybeans. The results showed that as the degree of drought stress was aggravated, all of the indices including leaf area, chlorophyll content, net photosynthetic rates (Pn), stomatal conductance (g(s)), transpiration rate (Tr), intercellular CO2 concentration (Ci), plant mass, plant height, seed yield, and harvest index in the two cultivars declined. The root length and root mass increased under light drought stress, and decreased under severe drought stress. Root-shoot ratio ascended as the degree of drought stress was aggravated. Under severe drought stress, the increase of root-shoot ratio of the drought-resistant soybean cultivar Jinda 70 was up to 135.7%, which was higher than the that (116.7%) of the drought-sensitive soybean cultivar Jindou 26. Simultaneously, leaf area and chlorophyll content in Jinda 70 were respectively 69.3% and 85.5% of those in the control, which were better than those of Jindou 26. g(s) and Pn of Jinda 70 respectively declined 67.9% and 77.9%, but still lower than those of Jindou 26. Therefore, the decline range of harvest index of Jinda 70 was 43.8%, which was lower than the range of 78.8% of Jindou 26. The Biplot revealed that under different dry treatments, there were significant positive correlations among the six indexes including leaf area, chlorophyll content, Pn, g(s), Tr, and Ci of the two cultivars. There were also significant positive correlations among the six indices including plant mass, plant height, root length, root mass, seed yield, and harvest index. Root-shoot ratio only had significant positive correlation with root

  19. Temperature Stress at Grain Filling Stage Mediates Expression of Three Isoform Genes Encoding Starch Branching Enzymes in Rice Endosperm

    Institute of Scientific and Technical Information of China (English)

    WEI Ke-su; CHENG Fang-min; ZHANG Qi-fang; LIU Kui-gang

    2009-01-01

    An early-maturity indica rice variety Zhefu 49, whose grain quality and starch structure are sensitive to environmental temperature, was subjected to different temperatures (32oC for high temperature and 22oC for optimum temperature) at the grain filling stage in plant growth chambers, and the different expressions of three isoform genes (SBEI, SBEIII and SBEIV) encoding starch branching enzyme (SBE) in the endosperms were studied by the real-time fluorescence quantitative PCR (FQ-PCR) method. Effects of high temperature on the SBE expression in developing rice endosperms were isoform-dependent. High temperature significantly down-regulated the expressions of SBEI and SBEIII, while up-regulated the expression of SBEIV. Compared with SBEIV and SBEIII, the expression of SBEI gene in Zhefu 49 rice endosperms was more sensitive to temperature variation at the grain filling stage. This study indicates that changes in weather/climate conditions especially temperature stress influence rice grain formation and its quality as evidenced by isoform expression.

  20. Relationship Between Variation in Activities of Key Enzymes Related to Starch Synthesis During Grain Filling Period and Quality of Eating and Cooking in Rice

    Institute of Scientific and Technical Information of China (English)

    SHEN Peng; QIAN Chun-rong; JIN Zheng-xun; LUO Qiu-xiang; JIN Xue-yong

    2006-01-01

    Four japonica rice varieties with significant differences in quality of eating and cooking were used in the experiment. The varieties showed differences in amylose and amyiopectin contents at different grain filling stages, which were attributed to the accumulative speed of starch at different grain filling stages. During grain filling period, the varieties had no difference in the time when the activities of ADPglocose pyrophosphorylase (AGPP) and soluble starch synthesis (SSS) reached a maximum, but had difference in the time when the activity of starch branching enzyme (SBE) reached a maximum, in which the inferior quality varieties were earlier than the high quality ones, and high quality varieties still kept high enzyme activities at the late stage of grain filling. The correlation and correlative degree between AGPP, SSS, SBE and amylose content, amylopectin content, taste meter value, and RVA properties varied with the different stages of grain filling. The correlation between SSS activity and taste meter value was not significant during the whole period of grain filling, but the activities of AGPP and SBE had significant or highly significant correlation with taste meter value. It was helpful for improving quality of eating and cooking of japonica rice to use the materials with low enzyme activity at the early stage of grain filling or high enzyme activity at the late stage as parents.

  1. 玉米高产品种籽粒灌浆特性研究%Study on Grain Filling Characteristics of High-Yielding Maize Cultivars

    Institute of Scientific and Technical Information of China (English)

    吴秋平; 郭新平; 韩成卫; 王惠芳; 孔晓民

    2015-01-01

    以郑单958为对照,对3个高产玉米品种籽粒灌浆特性进行研究,并运用Logistic生长模型拟合籽粒灌浆过程(R2>0.99),结果表明:迪卡517、登海605和金海5号较郑单958(CK)达到灌浆峰值后的灌浆速率下降相对较慢;籽粒灌浆速率和干物质积累量在籽粒生长各阶段均存在品种间差异,3个高产品种的最大灌浆速率、灌浆速率最大时生长量和平均灌浆速率均明显高于郑单958,而灌浆活跃期各品种表现与之接近。籽粒灌浆速率的提高,尤其是快增期灌浆速率高,利于缩短后期库容建成时间,促进籽粒快速灌浆,从而能进一步发挥品种增产潜力。%In this experiment, the grain filling characteristics of three high -yielding maize cultivars were studied with ZD958 as control.The Logistic growth equation was used for the fitting of grain filling process(R2 >0.99).The results showed that the grain filling process of the four cultivars showed a single peak curve consistently.Compared to ZD958, the grain filling rate of DK517, DH605 and JH5 decreased more slowly after the peak.The grain filling rate and dry matter accumulation were different in different grain growth period between different cultivars.The maximum and average grain filling rate and the grain weight at the stage with the maximum grain filling rate were all higher than those of ZD958;but in the active grain filling period, the performance of the three cultivars were same with that of ZD958.Therefore, enhancing the grain filling rate, especially in the rapidly increasing phase of grain weight, could accelerate the whole grain filling progress to shorten the duration of late grain filling,and maybe contribute to reaching a higher yield potential of maize hybrids.

  2. Carbohydrate composition and in vitro digestibility of dry matter and non-starch polysaccharides in corn, sorghum, and wheat, and co-products from these grains

    DEFF Research Database (Denmark)

    Jaworski, N. A.; Lærke, Helle Nygaard; Knudsen, Knud Erik Bach

    2015-01-01

    The objectives of this work were to determine carbohydrate composition and in vitro digestibility of DM and nonstarch polysaccharides (NSP) in corn, wheat, and sorghum and coproducts from these grains. In the initial part of this work, the carbohydrate composition of 12 feed ingredients was deter......The objectives of this work were to determine carbohydrate composition and in vitro digestibility of DM and nonstarch polysaccharides (NSP) in corn, wheat, and sorghum and coproducts from these grains. In the initial part of this work, the carbohydrate composition of 12 feed ingredients...... was determined. The 12 ingredients included 3 grains (corn, sorghum, and wheat), 3 coproducts from the dry grind industry (corn distillers dried grains with solubles [DDGS] and 2 sources of sorghum DDGS), 4 coproducts from the wet milling industry (corn gluten meal, corn gluten feed, corn germ meal, and corn...... up approximately 22, 49, and 29% (DM basis), respectively, of the NSP in corn and corn coproducts and approximately 25, 43, and 32% (DM basis), respectively, of the NSP in sorghum and sorghum DDGS. Cellulose, arabinoxylans, and other hemicelluloses made up approximately 16, 64, and 20% (DM basis...

  3. Pre-anthesis high temperature acclimation alleviates the negative effects of postanthesis heat stress on stem stored carbohydrates remobilization and grain starch accumulation in wheat

    DEFF Research Database (Denmark)

    Wang, Xiao; Cai, Jian; Liu, Fulai

    2012-01-01

    The potential role of pre-anthesis high temperature acclimation in alleviating the negative effects of post-anthesis heat stress on stem stored carbohydrate remobilization and grain starch accumulation in wheat was investigated. The treatments included no heat-stress (CC), heat stress at pre-anth...

  4. Microbial degradation of whole-grain complex carbohydrates and impact on short-chain fatty acids and health.

    Science.gov (United States)

    Bach Knudsen, Knud Erik

    2015-03-01

    Whole-grain cereals have a complex dietary fiber (DF) composition consisting of oligosaccharides (mostly fructans), resistant starch, and nonstarch polysaccharides (NSPs); the most important are arabinoxylans, mixed-linkage β(1,3; 1,4)-d-glucan (β-glucan), and cellulose and the noncarbohydrate polyphenolic ether lignin. The highest concentration of NSPs and lignin is found in the outer cell layers of the grain, and refined flour will consequently be depleted of a large proportion of insoluble DF components. The flow and composition of carbohydrates to the large intestine are directly related to the intake of DF. The type and composition of cereal DF can consequently be used to modulate the microbial composition and activity as well as the production and molar ratios of short-chain fatty acids (SCFAs). Arabinoxylans and β-glucan in whole-grain cereals and cereal ingredients have been shown to augment SCFA production, with the strongest relative effect on butyrate. When arabinoxylans were provided as a concentrate, the effect was only on total SCFA production. Increased SCFA production in the large intestine was shown by the concentration in the portal vein, whereas the impact on the concentration in peripheral blood was less because the majority of propionate and butyrate is cleared in the liver. Active microbial fermentation with increased SCFA production reduced the exposure of potentially toxic compounds to the epithelium, potentially stimulating anorectic hormones and acting as signaling molecules between the gut and the peripheral tissues. The latter can have implications for insulin sensitivity and glucose homeostasis.

  5. Within-leaf nitrogen allocation in adaptation to low nitrogen supply in maize during grain-filling stage

    Directory of Open Access Journals (Sweden)

    Xiaohuan eMu

    2016-05-01

    Full Text Available Nitrogen (N plays a vital role in photosynthesis and crop productivity. Maize plants may be able to increase physiological N utilization efficiency (NUtE under low-N stress by increasing photosynthetic rate (Pn per unit leaf N, that is, photosynthetic N-use efficiency (PNUE. In this study, we analyzed the relationship between PNUE and N allocation in maize ear-leaves during the grain-filling stage under low N (no N application and high N (180 kg N ha−1 in a 2-year field experiment. Under low N, grain yield decreased while NUtE increased. Low-N treatment reduced the specific N content of ear leaves by 38% without significant influencing Pn, thereby increasing PNUE by 54%. Under low-N stress, maize plants tended to invest relatively more N into bioenergetics to sustain electron transport. In contrast, N allocated to chlorophyll and light-harvesting proteins was reduced to control excess electron production. Soluble proteins were reduced to shrink the N storage reservoir. We conclude that optimization of N allocation within leaves is a key adaptive mechanism to maximize Pn and crop productivity when N is limited during the grain-filling stage in maize under low-N conditions.

  6. Excessive nitrogen application dampens antioxidant capacity and grain filling in wheat as revealed by metabolic and physiological analyses

    Science.gov (United States)

    Kong, Lingan; Xie, Yan; Hu, Ling; Si, Jisheng; Wang, Zongshuai

    2017-01-01

    In this study, field-grown wheat (Triticum aestivum L.) was treated with normal (Nn) and excessive (Ne) levels of fertilizer N. Results showed that Ne depressed the activity of superoxide dismutase and peroxidase and increased the accumulation of reactive oxygen species (ROS) and malondialdehyde. The normalized difference vegetation index (NDVI) was higher under Ne at anthesis and medium milk but similar at the early dough stage and significantly lower at the hard dough stage than that under Nn. The metabolomics analysis of the leaf responses to Ne during grain filling showed 99 metabolites that were different between Ne and Nn treatments, including phenolic and flavonoid compounds, amino acids, organic acids and lipids, which are primarily involved in ROS scavenging, N metabolism, heat stress adaptation and disease resistance. Organic carbon (C) and total N contents were affected by the Ne treatment, with lower C/N ratios developing after medium milk. Ultimately, grain yields decreased with Ne. Based on these data, compared with the normal N fertilizer treatment, we concluded that excessive N application decreased the ability to scavenge ROS, increased lipid peroxidation and caused significant metabolic changes disturbing N metabolism, secondary metabolism and lipid metabolism, which led to reduced grain filling in wheat. PMID:28233811

  7. Drought priming at vegetative growth stages improves tolerance to drought and heat stresses occurring during grain filling in spring wheat

    DEFF Research Database (Denmark)

    Wang, Xiao; Vignjevic, Marija; Liu, Fulai

    2015-01-01

    . Comparedwith the non-primed plants, drought priming could alleviate photo-inhibition in flag leaves caused by drought and heat stress episodes during grain filling. In the primed plants, drought stress inhibited photosynthesis mainly through decrease of maximum photosynthetic electron transport rate, while...... decrease of the carboxylation efficiency limited photosynthesis under heat stress. The higher saturated net photosynthetic rate of flag leaves coincidedwith the lowered nonphotochemical quenching rates in the twice-primed plants under drought stress and in the primed plants during stem elongation under...

  8. Abscisic acid and aldehyde oxidase activity in maize ear leaf and grain relative to post-flowering photosynthetic capacity and grain-filling rate under different water/nitrogen treatments.

    Science.gov (United States)

    Qin, Shujun; Zhang, Zongzheng; Ning, Tangyuan; Ren, Shizhong; Su, Licheng; Li, Zengjia

    2013-09-01

    This study investigated changes in leaf abscisic acid (ABA) concentrations and grain ABA concentrations in two maize cultivars and analyzed the following relationships under different water/nitrogen treatments: leaf ABA concentrations and photosynthetic parameters; leaf ABA concentrations and grain ABA concentrations; leaf/grain ABA concentrations and grain-filling parameters; and aldehyde oxidase (AO, EC 1.2.3.1) activities and ABA concentrations. The ear leaf average AO activities and ABA concentrations were lower in the controlled release urea treatments compared with the conventional urea treatments. The average AO activities in the grains were higher in the controlled release urea treatments, and the ABA concentrations were significantly increased at 11-30 DAF. The Pn and ABA concentrations in ear leaves were negatively correlated. And the Gmean were positively correlated with the grain ABA concentrations at 11-30 DAF and negatively correlated with the leaf ABA concentrations at 20 and 40-50 DAF. The grain ABA concentrations and leaf ABA concentrations were positively correlated. Thus, the Gmean were closely related to the AO activities and to the ear leaf and grain ABA concentrations. As compared to other treatments, the subsoiling and controlled release urea treatment promoted the uptake of water and nitrogen by maize, increased the photosynthetic capacity of the ear leaves, increased the grain-filling rate, and improved the movement of photosynthetic assimilates toward the developing grains. In the cultivar Z958, higher ABA concentrations in grains at 11-30 DAF and lower ABA concentrations in ear leaves during the late grain-filling stage, resulted in higher grain-filling rate and increased accumulation of photosynthetic products (relative to the cultivar D3).

  9. Changes in Enzyme Activities Involved in Starch Synthesis and Hormone Concentrations in Superior and Inferior Spikelets and Their Association with Grain Filling of Super Rice

    Institute of Scientific and Technical Information of China (English)

    FU Jing; XU Yun-ji; CHEN Lu; YUAN Li-min; WANG Zhi-qin; YANG Jian-chang

    2013-01-01

    The changes in activities of key enzymes involved in sucrose-to-starch conversion and concentrations of hormones in superior and inferior spikelets of super rice were investigated and their association with grain filling was analyzed.Four super rice cultivars,Liangyoupeijiu,Ilyou 084,Huaidao 9 and Wujing 15,and two high-yielding and elite check cultivars,Shanyou 63 and Yangfujing 8,were used.The activities of sucrose synthase (SuSase),adenosine diphosphoglucose pyrophosphorylase (AGPase),starch synthase (StSase) and starch branching enzyme (SBE),and the concentrations of zeatin + zeatin riboside (Z + ZR),indole-3-acetic acid (IAA) and abscisic acid (ABA) in superior and inferior spikelets were determined during the grain filling period and their relationships with grain filling rate were analyzed.Maximum grain filling rate,the time reaching the maximum grain-filling rate,mean grain filling rate and brown rice weight for superior spikelets showed a slight difference between the super and check rice cultivars,but were significantly lower in the super rice than in the check rice for inferior spikelets.Changes of enzyme activities and hormone concentrations in grains exhibited single peak curves during the grain filling period.The peak values and the mean activities of SuSase,AGPase,StSase and SBE were lower in inferior spikelets than in superior ones,as well as the peak values and the mean concentrations of Z + ZR and IAA.However,the peak value and the mean concentration of ABA were significantly higher in inferior spikelets than in superior ones and greater in the super rice than in the check rice.The grain filling rate was positively and significantly correlated with the activities of SuSase,AGPase and StSase and the concentrations of Z + ZR and IAA.The results suggested that the low activities of SuSase,AGPase and StSase and the low concentrations of Z + ZR and IAA might be important physiological reasons for the slow grain filling rate and light grain weight of

  10. Physiological and proteome studies of responses to heat stress during grain filling in contrasting wheat cultivars

    DEFF Research Database (Denmark)

    Wang, Xiao; Dinler, Burcu Seckin; Vignjevic, Marija;

    2015-01-01

    Experiments to explore physiological and biochemical differences of the effects of heat stress in ten wheat (Triticum aestivum L) cultivars have been performed. Based on the response of photosynthesis rates, cell membrane lipid peroxide concentrations and grain yield to heat, six cultivars were...... compared to sensitive cultivars under heat stress. The tolerant cv. '810' and the sensitive cv. '1039' were selected for further proteome analysis of leaves. Proteins related to photosynthesis, glycolysis, stress defence, heat shock and ATP production were differently expressed in leaves of the tolerant...... and sensitive cultivar under heat stress in relation to the corresponding control. The abundance of proteins related to signal transduction, heat shock, photosynthesis, and antioxidants increased, while the abundance of proteins related to nitrogen metabolism decreased in the tolerant cv. '810' under heat...

  11. Carbohydrate composition and in vitro digestibility of dry matter and nonstarch polysaccharides in corn, sorghum, and wheat and coproducts from these grains.

    Science.gov (United States)

    Jaworski, N W; Lærke, H N; Bach Knudsen, K E; Stein, H H

    2015-03-01

    The objectives of this work were to determine carbohydrate composition and in vitro digestibility of DM and nonstarch polysaccharides (NSP) in corn, wheat, and sorghum and coproducts from these grains. In the initial part of this work, the carbohydrate composition of 12 feed ingredients was determined. The 12 ingredients included 3 grains (corn, sorghum, and wheat), 3 coproducts from the dry grind industry (corn distillers dried grains with solubles [DDGS] and 2 sources of sorghum DDGS), 4 coproducts from the wet milling industry (corn gluten meal, corn gluten feed, corn germ meal, and corn bran), and 2 coproducts from the flour milling industry (wheat middlings and wheat bran). Results indicated that grains contained more starch and less NSP compared with grain coproducts. The concentration of soluble NSP was low in all ingredients. Cellulose, arabinoxylans, and other hemicelluloses made up approximately 22, 49, and 29% (DM basis), respectively, of the NSP in corn and corn coproducts and approximately 25, 43, and 32% (DM basis), respectively, of the NSP in sorghum and sorghum DDGS. Cellulose, arabinoxylans, and other hemicelluloses made up approximately 16, 64, and 20% (DM basis), respectively, of the NSP in wheat and wheat coproducts. The concentration of lignin in grains was between 0.8 and 1.8% (DM basis), whereas coproducts contained between 2.2 and 11.5% lignin (DM basis). The in vitro ileal digestibility of NSP was close to zero or negative for all feed ingredients, indicating that pepsin and pancreas enzymes have no effect on in vitro degradation of NSP. A strong negative correlation ( = 0.97) between in vitro ileal digestibility of DM and the concentration of NSP in feed ingredients was observed. In vitro total tract digestibility of NSP ranged from 6.5% in corn bran to 57.3% in corn gluten meal. In conclusion, grains and grain coproducts contain mostly insoluble NSP and arabinoxylans make up the majority of the total NSP fraction. The in vitro

  12. Analysis of gluten proteins composition during grain filling in two durum wheat cultivars submitted to two water regimes

    Directory of Open Access Journals (Sweden)

    Marcella Michela Giuliani

    2014-02-01

    Full Text Available Durum wheat (Triticum turgidum L. subsp. durum is one of the major crops in the Mediterranean basin, where water stress often occurs during grain filling which represents a critical stage for the synthesis and accumulation of storage proteins (gliadins and glutenins. The aim of the study is to evaluate, by two-dimensional gel electrophoresis (2DE SDS-PAGE, the storage proteins composition of two durum wheat cultivars (Ciccio and Svevo cultivated in a growth chamber under two different water regimes (control and water deficit. At milk stage and physiological maturity, gluten proteins have been extracted and separated by 2DE SDS-PAGE. The analysis of the gels was performed by the software ImageMaster 2D Platinum (Amersham. The results showed differences in protein expression within the different gel regions between water regimes and cultivars; under water deficit the rate of protein accumulation was faster for all the protein regions, either at milk and physiological stage. Protein accumulation within high molecular weight (H region resulted faster in Ciccio than in Svevo mainly in the control treatment. In the low molecular weight region between 48 and 35 kDa (L 48-35, the cultivar Ciccio showed a higher protein expression than Svevo. Furthermore under water deficit a marked increase in H region volume and a decrease in the L 48-35 region was observed only for Svevo; instead in Ciccio no change was observed showing this cultivar a greater stability on changing water regime. Further studies by the use of mass spectrometry are necessary to identify specific peptides relative to drought stress during grain filling as well as to investigate the relationships with technological quality.

  13. Use of the Stable Nitrogen Isotope to Reveal the Source-Sink Regulation of Nitrogen Uptake and Remobilization during Grain Filling Phase in Maize

    Science.gov (United States)

    Yang, Lan; Guo, Song; Chen, Qinwu; Chen, Fanjun; Yuan, Lixing; Mi, Guohua

    2016-01-01

    Although the remobilization of vegetative nitrogen (N) and post-silking N both contribute to grain N in maize (Zea mays L.), their regulation by grain sink strength is poorly understood. Here we use 15N labeling to analyze the dynamic behaviors of both pre- and post-silking N in relation to source and sink manipulation in maize plants. The results showed that the remobilization of pre-silking N started immediately after silking and the remobilized pre-silking N had a greater contribution to grain N during early grain filling, with post-silking N importance increasing during the later filling stage. The amount of post-silking N uptake was largely driven by post-silking dry matter accumulation in both grain as well as vegetative organs. Prevention of pollination during silking had less effect on post-silking N uptake, as a consequence of compensatory growth of stems, husk + cob and roots. Also, leaves continuously export N even though grain sink was removed. The remobilization efficiency of N in the leaf and stem increased with increasing grain yield (hence N requirement). It is suggested that the remobilization of N in the leaf is controlled by sink strength but not the leaf per se. Enhancing post-silking N uptake rather than N remobilization is more likely to increase grain N accumulation. PMID:27606628

  14. The Effect of Plastic-Covered Ridge and Furrow Planting on the Grain Filling and Hormonal Changes of Winter Wheat

    Institute of Scientific and Technical Information of China (English)

    LIU Yang; HAN Juan; WEN Xiao-xia; WU Wei; GUO Qiang; ZENG Ai; LIAO Yun-cheng

    2013-01-01

    Although plastic-covered ridge and furrow planting (RF) has been reported to produce substantial increases in the grain weight of winter wheat, the underlying mechanism is not yet understood. The present study used two cultivars, Xinong 538 and Zhoumai 18, and RF and traditional lfatten planting (TF, control) with the objective of investigating the effect of RF on wheat grain iflling and the possible relationship of hormonal changes in the wheat grains under RF to grain iflling. The results indicated that RF signiifcantly increased the grain weight, although the effects on grain iflling were different: RF signiifcantly increased the grain-iflling rate and grain weight of inferior grains, whereas RF had no signiifcant effect on grain-iflling rate and grain weight of superior grains. The ifnal grain weight of inferior grains under RF was 39.1 and 50.7 mg for Xinong 538 and Zhoumai 18, respectively, 3.6 and 3.4 mg higher than the values under TF. However, the ifnal grain weight of superior grains under RF was only 0.6 and 0.8 mg higher than under TF for Xinong 538 and Zhoumai 18, respectively. RF signiifcantly decreased the ethylene and gibberellic acid content in the inferior grains and increased the indole-3-acetic acid, abscisic acid and zeatin+zeatin riboside content in the inferior grains; however, no signiifcant difference between RF and TF was observed for the hormonal content in the superior grains. Based on these results, we concluded that RF signiifcantly modulated hormonal changes in the inferior grains and, thus, affected the grain iflling and grain weight of the inferior grains;in contrast, RF had no signiifcant effect on grain iflling, grain weight and hormonal changes in the superior wheat grains.

  15. Starch granule formation and protein deposition in wheat (Triticum aestivum L.) starchy endosperm cells is altered by high temperature during grain fill

    Science.gov (United States)

    Hurkman, William J.; Wood, Delilah F.

    2010-06-01

    High temperatures during wheat grain fill decrease starch and protein levels, adversely affecting wheat yield and flour quality. To determine the effect of high temperature on starchy endosperm cell development, grain (Triticum aestivum L. 'Butte 86') was produced under a 24/17°C or 37/28°C day/night regimen imposed from flowering to maturity and starch and protein deposition examined using scanning electron microscopy. The high temperature regimen shortened the duration of grain fill from 40 to 18 days. Under the 37/28°C regimen, A- and B-type starch granules decreased in size. A-type starch granules also exhibited pitting, suggesting enhanced action of starch degradative enzymes. Under both temperature regimens, protein bodies originated early in development and coalesced during mid to late development to form a continuous protein matrix surrounding the starch granules. Under the 37/28°C regimen, the proportion of protein matrix increased in endosperm cells of mature grain. Taken together, the changes in starch granule number and size and in protein matrix amount provide clues for understanding how high temperature during grain fill can affect end use properties of wheat flour.

  16. Response of rice plants to heat stress during initiation of panicle primordia or grain-filling phases

    Directory of Open Access Journals (Sweden)

    Hermann Restrepo-Diaz

    2013-08-01

    Full Text Available Leaf photosynthesis, a major determinant for yield sustainability in rice, is greatly conditioned by high temperature stress during growth. The effect of short-term high temperatures on leaf photosynthesis, stomatal conductance, Fv/Fm, SPAD readings and yield characteristics was studied in two Colombian rice cultivars. Two genotypes, cv. Fedearroz 50 (F50 and cv. Fedearroz 733 (F733 were used in pot experiments with heat stress treatment (Plants were exposed to 40°C for two and half hours for five consecutive days and natural temperature (control treatment. Heat treatments were carried out at the initiation of panicle primordial (IP or grain-filling (GF phases. The results showed that short-term high temperature stress produced a reduction on the photosynthesis rate in both cultivars either IP or GF phases. Similar trends were found on stomatal conductance in all cases due to high temperatures. Although Fv/Fm and SPAD readings were not affected by high temperatures, these variables diminished significantly among phenological phases. 'F733' rice plants showed higher number spikelet sterility due to heat stress treatments. These results seem to indicate that heat-tolerant cultivars of rice is associated with high levels of photosynthesis rate in leaves.

  17. Identification and Characterization of Differentially Expressed Genes in Inferior and Superior Spikelets of Rice Cultivars with Contrasting Panicle-Compactness and Grain-Filling Properties.

    Directory of Open Access Journals (Sweden)

    Sudhanshu Sekhar

    Full Text Available Breeding programs for increasing spikelet number in rice have resulted in compactness of the panicle, accompanied by poor grain filling in inferior spikelets. Although the inefficient utilization of assimilate has been indicated as responsible for this poor grain filling, the underlying cause remains elusive. The current study utilized the suppression subtractive hybridization technique to identify 57 and 79 genes that overexpressed in the superior and inferior spikelets (with respect to each other, respectively, of the compact-panicle rice cultivar Mahalaxmi. Functional categorization of these differentially expressed genes revealed a marked metabolic difference between the spikelets according to their spatial location on the panicle. The expression of genes encoding seed storage proteins was dominant in inferior spikelets, whereas genes encoding regulatory proteins, such as serine-threonine kinase, zinc finger protein and E3 ligase, were highly expressed in superior spikelets. The expression patterns of these genes in the inferior and superior spikelets of Mahalaxmi were similar to those observed in another compact-panicle cultivar, OR-1918, but differed from those obtained in two lax-panicle cultivars, Upahar and Lalat. The results first suggest that the regulatory proteins abundantly expressed in the superior spikelets of compact-panicle cultivars and in both the superior and inferior spikelets of lax-panicle cultivars but poorly expressed in the inferior spikelets of compact-panicle cultivars promote grain filling. Second, the high expression of seed-storage proteins observed in the inferior spikelets of compact-panicle cultivars appears to inhibit the grain filling process. Third, the low expression of enzymes of the Krebs cycle in inferior spikelets compared with superior spikelets of compact-panicle cultivars is bound to lead to poor ATP generation in the former and consequently limit starch biosynthesis, an ATP-consuming process

  18. Changes in Whole-Plant Metabolism during the Grain-Filling Stage in Sorghum Grown under Elevated CO2 and Drought.

    Science.gov (United States)

    De Souza, Amanda P; Cocuron, Jean-Christophe; Garcia, Ana Carolina; Alonso, Ana Paula; Buckeridge, Marcos S

    2015-11-01

    Projections indicate an elevation of the atmospheric CO2 concentration ([CO2]) concomitant with an intensification of drought for this century, increasing the challenges to food security. On the one hand, drought is a main environmental factor responsible for decreasing crop productivity and grain quality, especially when occurring during the grain-filling stage. On the other hand, elevated [CO2] is predicted to mitigate some of the negative effects of drought. Sorghum (Sorghum bicolor) is a C4 grass that has important economical and nutritional values in many parts of the world. Although the impact of elevated [CO2] and drought in photosynthesis and growth has been well documented for sorghum, the effects of the combination of these two environmental factors on plant metabolism have yet to be determined. To address this question, sorghum plants (cv BRS 330) were grown and monitored at ambient (400 µmol mol(-1)) or elevated (800 µmol mol(-1)) [CO2] for 120 d and subjected to drought during the grain-filling stage. Leaf photosynthesis, respiration, and stomatal conductance were measured at 90 and 120 d after planting, and plant organs (leaves, culm, roots, prop roots, and grains) were harvested. Finally, biochemical composition and intracellular metabolites were assessed for each organ. As expected, elevated [CO2] reduced the stomatal conductance, which preserved soil moisture and plant fitness under drought. Interestingly, the whole-plant metabolism was adjusted and protein content in grains was improved by 60% in sorghum grown under elevated [CO2].

  19. A multi-disciplinary study of deformation of the basaltic cover over fine-grained valley fills: a case study from Eastern Sardinia, Italy

    Science.gov (United States)

    Deiana, Rita; Dieni, Iginio; Massari, Francesco; Perri, Maria Teresa; Rossi, Matteo; Brovelli, Alessandro

    2016-06-01

    The Pliocene to Early Pleistocene volcanic activity which generated the basaltic plateau of the Orosei-Dorgali area in Eastern Sardinia led to the disruption of the local hydrographic network by damming some tracts of the fluvial valleys incised in the granite basement. This resulted in the formation of lacustrine basins, whose fine-grained fills were partly interfingered and eventually covered by younger lava flows. In the SW part of the plateau, close to the Galtellì village, a number of unknown depressions, locally named "Paules," were formed. In order to reconstruct their subsurface structure, two electrical resistivity tomography surveys were carried out across these depressions. The geophysical results, which demonstrate the existence of a disrupted layered system, were used to build a numerical geomechanical model that suggest the depressions originated by local collapses of the basaltic cover due to the compaction of the underlying fine-grained valley fills.

  20. Route and Regulation of Zinc, Cadmium, and Iron Transport in Rice Plants (Oryza sativa L. during Vegetative Growth and Grain Filling: Metal Transporters, Metal Speciation, Grain Cd Reduction and Zn and Fe Biofortification

    Directory of Open Access Journals (Sweden)

    Tadakatsu Yoneyama

    2015-08-01

    Full Text Available Zinc (Zn and iron (Fe are essential but are sometimes deficient in humans, while cadmium (Cd is toxic if it accumulates in the liver and kidneys at high levels. All three are contained in the grains of rice, a staple cereal. Zn and Fe concentrations in rice grains harvested under different levels of soil/hydroponic metals are known to change only within a small range, while Cd concentrations show greater changes. To clarify the mechanisms underlying such different metal contents, we synthesized information on the routes of metal transport and accumulation in rice plants by examining metal speciation, metal transporters, and the xylem-to-phloem transport system. At grain-filling, Zn and Cd ascending in xylem sap are transferred to the phloem by the xylem-to-phloem transport system operating at stem nodes. Grain Fe is largely derived from the leaves by remobilization. Zn and Fe concentrations in phloem-sap and grains are regulated within a small range, while Cd concentrations vary depending on xylem supply. Transgenic techniques to increase concentrations of the metal chelators (nicotianamine, 2′-deoxymugineic acid are useful in increasing grain Zn and Fe concentrations. The elimination of OsNRAMP5 Cd-uptake transporter and the enhancement of root cell vacuolar Cd sequestration reduce uptake and root-to-shoot transport, respectively, resulting in a reduction of grain Cd accumulation.

  1. Route and Regulation of Zinc, Cadmium, and Iron Transport in Rice Plants (Oryza sativa L.) during Vegetative Growth and Grain Filling: Metal Transporters, Metal Speciation, Grain Cd Reduction and Zn and Fe Biofortification.

    Science.gov (United States)

    Yoneyama, Tadakatsu; Ishikawa, Satoru; Fujimaki, Shu

    2015-08-13

    Zinc (Zn) and iron (Fe) are essential but are sometimes deficient in humans, while cadmium (Cd) is toxic if it accumulates in the liver and kidneys at high levels. All three are contained in the grains of rice, a staple cereal. Zn and Fe concentrations in rice grains harvested under different levels of soil/hydroponic metals are known to change only within a small range, while Cd concentrations show greater changes. To clarify the mechanisms underlying such different metal contents, we synthesized information on the routes of metal transport and accumulation in rice plants by examining metal speciation, metal transporters, and the xylem-to-phloem transport system. At grain-filling, Zn and Cd ascending in xylem sap are transferred to the phloem by the xylem-to-phloem transport system operating at stem nodes. Grain Fe is largely derived from the leaves by remobilization. Zn and Fe concentrations in phloem-sap and grains are regulated within a small range, while Cd concentrations vary depending on xylem supply. Transgenic techniques to increase concentrations of the metal chelators (nicotianamine, 2'-deoxymugineic acid) are useful in increasing grain Zn and Fe concentrations. The elimination of OsNRAMP5 Cd-uptake transporter and the enhancement of root cell vacuolar Cd sequestration reduce uptake and root-to-shoot transport, respectively, resulting in a reduction of grain Cd accumulation.

  2. Structure and properties of fluid-filled grain boundaries under stress in geological materials. Geologica Ultraiectina (290)

    OpenAIRE

    Van de Noort, R.

    2008-01-01

    Two of the three processes making up the deformation mechanism of intergranular pressure solution, being dissolution and diffusion, take place in the grain boundary fluid phase. Hence, the structure and physical properties of wet grain boundaries under stress can be expected to influence the kinetics of both dissolution and diffusion, as a result of effects such as molecular structuring of the fluid film, the grain boundary surface area available for dissolution, the dissipation of energy oth...

  3. Ear Rachis Xylem Occlusion and Associated Loss in Hydraulic Conductance Coincide with the End of Grain Filling for Wheat.

    Science.gov (United States)

    Neghliz, Hayet; Cochard, Hervé; Brunel, Nicole; Martre, Pierre

    2016-01-01

    Seed dehydration is the normal terminal event in the development of orthodox seeds and is physiologically related to the cessation of grain dry mass accumulation and crop grain yield. For a better understanding of grain dehydration, we evaluated the hypothesis that hydraulic conductance of the ear decreases during the latter stages of development and that this decrease results from disruption or occlusion of xylem conduits. Whole ear, rachis, and stem nodes hydraulic conductance and percentage loss of xylem conductivity were measured from flowering to harvest-ripeness on bread wheat (Triticum aestivum L.) cv. Récital grown under controlled environments. Flag leaf transpiration, stomatal conductance, chlorophyll content and grain and ear water potentials were also measured during grain development. We show that grain dehydration was not related with whole plant physiology and leaf senescence, but closely correlated with the hydraulic properties of the xylem conduits irrigating the grains. Indeed, there was a substantial decrease in rachis hydraulic conductance at the onset of the grain dehydration phase. This hydraulic impairment was not caused by the presence of air embolism in xylem conduits of the stem internodes or rachis but by the occlusion of the xylem lumens by polysaccharides (pectins and callose). Our results demonstrate that xylem hydraulics plays a key role during grain maturation.

  4. Microbial degradation of whole-grain complex carbohydrates and impact on short-chain fatty acids and health

    DEFF Research Database (Denmark)

    Knudsen, Knud Erik Bach

    2015-01-01

    to the intake of DF. The type and composition of cereal DF can consequently be used to modulate the microbial composition and activity as well as the production and molar ratios of short-chain fatty acids (SCFAs). Arabinoxylans and β-glucan in whole-grain cereals and cereal ingredients have been shown...

  5. Activities of starch hydrolytic enzymes and sucrose-phosphate synthase in the stems of rice subjected to water stress during grain filling.

    Science.gov (United States)

    Yang, J; Zhang, J; Wang, Z; Zhu, Q

    2001-11-01

    To understand the effect of water stress on the remobilization of prestored carbon reserves, the changes in the activities of starch hydrolytic enzymes and sucrose-phosphate synthase (SPS) in the stems of rice (Oryza sativa L.) during grain filling were investigated. Two rice cultivars, showing high lodging-resistance and slow remobilization, were grown in the field and subjected to well-watered (WW, psi(soil)=0) and water-stressed (WS, psi(soil)=-0.05 MPa) treatments 9 d after anthesis (DAA) till maturity. Leaf water potentials of both cultivars markedly decreased during the day as a result of WS treatment, but completely recovered by early morning. WS treatment accelerated the reduction of starch in the stems, promoted the reallocation of prefixed (14)C from the stems to grains, shortened the grain filling period, and increased the grain filling rate. More soluble sugars including sucrose were accumulated in the stems under WS than under WW treatments. Both alpha- and beta-amylase activities were enhanced by the WS, with the former enhanced more than the latter, and were significantly correlated with the concentrations of soluble sugars in the stems. The other two possible starch-breaking enzymes, alpha-glucosidase and starch phosphorylase, showed no significant differences in the activities between the WW and WS treatments. Water stress also increased the SPS activity that is responsible for sucrose production. Both V(limit) and V(max), the activities of the enzyme at limiting and saturating substrate concentrations, were enhanced and the activation state (V(limit)/V(max)) was also increased as a result of the more significant enhancement of V(limit). The enhanced SPS activity was closely correlated with an increase of sucrose accumulation in the stems. The results suggest that the fast hydrolysis of starch and increased carbon remobilization were attributed to the enhanced alpha-amylase activity and the high activation state of SPS when the rice was subjected

  6. Integrating statistical and ecophysiological analysis of genotype by environment interaction for grain filling of barley in Mediterranean areas. II. Grain growth

    NARCIS (Netherlands)

    Voltas, J.; Eeuwijk, van F.A.; Araus, J.L.; Romagosa, I.

    1999-01-01

    In Mediterranean areas, grain growth of temperate cereals often progresses under the harmful influence of drought and high temperature. Genotypic responses are mediated by the specific occurrence of these constraints, thus causing genotype by environment (G × E) interaction. Field experiments were c

  7. Integrating statistical and ecophysiological analysis of genotype by environment interaction for grain filling of barley in Mediterranean areas. I. Individual grain weight

    NARCIS (Netherlands)

    Voltas, J.; Eeuwijk, van F.A.; Sombrero, A.; Lafarga, A.; Igartua, E.; Romagosa, I.

    1999-01-01

    In Mediterranean-type environments, grain weight of barley (Hordeum vulgare L.) is influenced by the presence of terminal abiotic stresses such as drought or elevated temperature. The relative importance of these climatic constraints acting together prior to and after anthesis, as well as the specif

  8. Effect of High Temperature on Sucrose Content and Sucrose Cleaving Enzyme Activity in Rice Grain During the Filling Stage

    Institute of Scientific and Technical Information of China (English)

    LI Tian; LIU Qi-hua; Ryu OHSUGI; Tohru YAMAGISHI; Haruto SASAKI

    2006-01-01

    Dynamic changes of sucrose, fructose, glucose contents and differences in activities of sucrose synthase, vacuolar invertase, and cell wall bound invertase in rice grain after flowering stage were studied under natural and high temperatures by using two japonica rice varieties Koshihikari and Sasanishiki. In rice grains, the sucrose synthase activity was higher than that of invertase, which was significantly correlated with starch accumulation rate, indicating that the sucrose synthase played an important role in sucrose degradation and starch synthesis. Under high temperature, the significant increase in grain sucrose content without any increase in fructose and glucose contents, suggested that the high temperature treatment enhanced sucrose accumulation, while diminished sucrose degradation in rice grains. Compared with the control plants, the decrease in activities of sucrose synthase, vacuolar invertase, and cell wall bound invertase with high temperature treated plants indicated that the deceleration of sucrose-degradation was related to the decrease in activities of sucrose synthase and invertase.

  9. Understanding the differences in molecular conformation of carbohydrate and protein in endosperm tissues of grains with different biodegradation kinetics using advanced synchrotron technology

    Science.gov (United States)

    Yu, P.; Block, H. C.; Doiron, K.

    2009-01-01

    Conventional "wet" chemical analyses rely heavily on the use of harsh chemicals and derivatization, thereby altering native seed structures leaving them unable to detect any original inherent structures within an intact tissue sample. A synchrotron is a giant particle accelerator that turns electrons into light (million times brighter than sunlight) which can be used to study the structure of materials at the molecular level. Synchrotron radiation-based Fourier transform IR microspectroscopy (SR-FTIRM) has been developed as a rapid, direct, non-destructive and bioanalytical technique. This technique, taking advantage of the brightness of synchrotron light and a small effective source size, is capable of exploring the molecular chemistry within the microstructures of a biological tissue without the destruction of inherent structures at ultraspatial resolutions within cellular dimensions. This is in contrast to traditional 'wet' chemical methods, which, during processing for analysis, often result in the destruction of the intrinsic structures of feeds. To date there has been very little application of this technique to the study of plant seed tissue in relation to nutrient utilization. The objective of this study was to use novel synchrotron radiation-based technology (SR-FTIRM) to identify the differences in the molecular chemistry and conformation of carbohydrate and protein in various plant seed endosperms within intact tissues at cellular and subcellular level from grains with different biodegradation kinetics. Barley grain (cv. Harrington) with a high rate (31.3%/h) and extent (78%), corn grain (cv. Pioneer) with a low rate (9.6%/h) and extent of (57%), and wheat grain (cv. AC Barrie) with an intermediate rate (23%/h) and extent (72%) of ruminal DM degradation were selected for evaluation. SR-FTIRM evaluations were performed at the National Synchrotron Light Source at the Brookhaven National Laboratory (Brookhaven, NY). The molecular structure spectral analysis

  10. Understanding the Differences in Molecular Conformation of Carbohydrate and Protein in Endosperm Tissues of Grains with Different Biodegradation Kinetics Using Advanced Synchrotron Technology

    Energy Technology Data Exchange (ETDEWEB)

    Yu, P.; Block, H; Doiron, K

    2009-01-01

    Conventional 'wet' chemical analyses rely heavily on the use of harsh chemicals and derivatization, thereby altering native seed structures leaving them unable to detect any original inherent structures within an intact tissue sample. A synchrotron is a giant particle accelerator that turns electrons into light (million times brighter than sunlight) which can be used to study the structure of materials at the molecular level. Synchrotron radiation-based Fourier transform IR microspectroscopy (SR-FTIRM) has been developed as a rapid, direct, non-destructive and bioanalytical technique. This technique, taking advantage of the brightness of synchrotron light and a small effective source size, is capable of exploring the molecular chemistry within the microstructures of a biological tissue without the destruction of inherent structures at ultraspatial resolutions within cellular dimensions. This is in contrast to traditional 'wet' chemical methods, which, during processing for analysis, often result in the destruction of the intrinsic structures of feeds. To date there has been very little application of this technique to the study of plant seed tissue in relation to nutrient utilization. The objective of this study was to use novel synchrotron radiation-based technology (SR-FTIRM) to identify the differences in the molecular chemistry and conformation of carbohydrate and protein in various plant seed endosperms within intact tissues at cellular and subcellular level from grains with different biodegradation kinetics. Barley grain (cv. Harrington) with a high rate (31.3%/h) and extent (78%), corn grain (cv. Pioneer) with a low rate (9.6%/h) and extent of (57%), and wheat grain (cv. AC Barrie) with an intermediate rate (23%/h) and extent (72%) of ruminal DM degradation were selected for evaluation. SR-FTIRM evaluations were performed at the National Synchrotron Light Source at the Brookhaven National Laboratory (Brookhaven, NY). These results suggest

  11. Whole-plant dynamic system of nitrogen use for vegetative growth and grain filling in rice plants (Oryza sativa L. as revealed through the production of 350 grains from a germinated seed over 150 days: a review and synthesis

    Directory of Open Access Journals (Sweden)

    Tadakatsu Yoneyama

    2016-08-01

    Full Text Available A single germinated rice (Oryza sativa L seed can produce 350 grains with the sequential development of 15 leaves on the main stem and 7 ‒ 10 leaves on 4 productive tillers (forming 5 panicles in total, using nitrogen (N taken up from the environment over a 150-day growing season. Nitrogen travels from uptake sites to the grain through growing organ-directed cycling among sequentially developed organs. Over the past 40 years, the dynamic system for N allocation during vegetative growth and grain filling has been elucidated through studies on N and 15N transport as well as enzymes and transporters involved. In this review, we synthesize the information obtained in these studies along the following main points: (1 During vegetative growth before grain-filling, about half of the total N in the growing organs, including young leaves, tillers, root tips and differentiating panicles is supplied via phloem from mature source organs such as leaves and roots, after turnover and remobilization of proteins, whereas the other half is newly taken up and supplied via xylem, with an efficient xylem-to-phloem transfer at stem nodes. Thus, the growth of new organs depends equally on both N sources. (2 A large fraction (as much as 80% of the grain N is derived largely from mature organs such as leaves and stems by degradation, including the autophagy pathway of chloroplast proteins (e.g., Rubisco. (3 Mobilized proteinogenic amino acids, including arginine, lysine, proline and valine, are derived mainly from protein degradation, with amino acid transporters playing a role in transferring these amino acids across cell membranes of source and sink organs, and enabling their efficient reutilization in the latter. On the other hand, amino acids such as glutamine, glutamic acid, γ-amino butyric acid, aspartic acid, and alanine are produced by assimilation of newly taken up N by roots and transported via xylem and phloem. The formation of 350 filled grains over 50

  12. Whole-Plant Dynamic System of Nitrogen Use for Vegetative Growth and Grain Filling in Rice Plants (Oryza sativa L.) as Revealed through the Production of 350 Grains from a Germinated Seed Over 150 Days: A Review and Synthesis.

    Science.gov (United States)

    Yoneyama, Tadakatsu; Tanno, Fumio; Tatsumi, Jiro; Mae, Tadahiko

    2016-01-01

    A single germinated rice (Oryza sativa L) seed can produce 350 grains with the sequential development of 15 leaves on the main stem and 7-10 leaves on four productive tillers (forming five panicles in total), using nitrogen (N) taken up from the environment over a 150-day growing season. Nitrogen travels from uptake sites to the grain through growing organ-directed cycling among sequentially developed organs. Over the past 40 years, the dynamic system for N allocation during vegetative growth and grain filling has been elucidated through studies on N and (15)N transport as well as enzymes and transporters involved. In this review, we synthesize the information obtained in these studies along the following main points: (1) During vegetative growth before grain-filling, about half of the total N in the growing organs, including young leaves, tillers, root tips and differentiating panicles is supplied via phloem from mature source organs such as leaves and roots, after turnover and remobilization of proteins, whereas the other half is newly taken up and supplied via xylem, with an efficient xylem-to-phloem transfer at stem nodes. Thus, the growth of new organs depends equally on both N sources. (2) A large fraction (as much as 80%) of the grain N is derived largely from mature organs such as leaves and stems by degradation, including the autophagy pathway of chloroplast proteins (e.g., Rubisco). (3) Mobilized proteinogenic amino acids (AA), including arginine, lysine, proline and valine, are derived mainly from protein degradation, with AA transporters playing a role in transferring these AAs across cell membranes of source and sink organs, and enabling their efficient reutilization in the latter. On the other hand, AAs such as glutamine, glutamic acid, γ-amino butyric acid, aspartic acid, and alanine are produced by assimilation of newly taken up N by roots and and transported via xylem and phloem. The formation of 350 filled grains over 50 days during the

  13. 水稻结实期籽粒和根系中玉米素与玉米素核苷含量的变化及其与籽粒充实的关系%Changes in Zeatin and Zeatin Riboside Content in Rice Grains and Roots during Grain Filling and the Relationship to Grain Plumpness

    Institute of Scientific and Technical Information of China (English)

    杨建昌; 彭少兵; 等

    2001-01-01

    Six rice cultivars (including 2 japonica/indica hybridcombinations and 1 new plant type line ) were grown in water culture in the green house /or in pots in the screen house. Zeatin (Z) and Zeatin riboside (ZR) contents in grains and roots during grain filling were measured with an enzyme-linked immunosorbent assay (ELISA), and the relationship to the grain filling were examined. Z+ZR content in grains was rather low at initial filling stage, and quickly increased with the filling process, and reached the peak at 9~15 days after flowering (DAF) for superior grains and 12~21 DAF for inferior ones, and sharply decreased thereafter. Z+ZR content in roots was rather high at heading, and reached the peak at 3~9 DAF. Z+ZR content in grains and roots, and the time reached the peak were associated with grain filling patterns of the cultivars. The cultivar with good grain plumpness and high grain filling rate had high Z+ZR content in grains and roots at early filling stage (3~12 DAF), and early reached the peak. Z+ZR content in grains and roots at early filling stage, the maximum content, and the mean content in whole filling period significantly or very significantly correlated with Fertilized grain-filling percentage (FGFP), grain weight, the maximum filling rate, and with mean filling rate. Z+ZR content in grains and roots, FGFP and grain weight were obviously decreased when roots were treated with NaN3 at heading, and the result was reversed when roots with 10-6mol/L zeatin. The results suggested that the grain filling pattern may be determined by the changing pattern of cytokinins (Z+ZR) in the grains and roots, and it is an effective approach to improving grain filling by regulating cytokinin levels in the grains and roots during grain filling, especially at early filling stage.%在水培和盆栽条件下,研究了6个水稻品种(含籼/粳杂交组合、新株型品系)结实期强、弱势粒和根系中玉米素(Z)与玉米素核苷(ZR)含量的变化

  14. Increased intake of carbohydrates from sources with a higher glycemic index and lower consumption of whole grains during puberty are prospectively associated with higher IL-6 concentrations in younger adulthood among healthy individuals.

    Science.gov (United States)

    Goletzke, Janina; Buyken, Anette E; Joslowski, Gesa; Bolzenius, Katja; Remer, Thomas; Carstensen, Maren; Egert, Sarah; Nöthlings, Ute; Rathmann, Wolfgang; Roden, Michael; Herder, Christian

    2014-10-01

    Chronic low-grade inflammation represents a likely intermediary in the relation between carbohydrate nutrition and both type 2 diabetes and cardiovascular disease. This study assessed the prospective association between carbohydrate quantity and quality [dietary glycemic index (GI), glycemic load (GL), and added sugar, fiber, and whole-grain intake] during puberty, a potentially critical period for later disease, and low-grade inflammation in younger adulthood. The analysis was based on 205 participants (113 girls and 92 boys) from the DONALD (Dortmund Nutritional and Anthropometric Longitudinally Designed) study with at least 2 3-d weighed dietary records during puberty (girls: 9-14 y, boys: 10-15 y) and blood samples in younger adulthood (18-36 y). Multivariable linear regression models were used to analyze the associations between carbohydrate nutrition and circulating concentrations of pro- and anti-inflammatory immune mediators [high-sensitivity C-reactive protein (hs-CRP), interleukin (IL) 6, IL-18, and adiponectin]. A higher intake of carbohydrates during puberty (P-trend = 0.005), particularly from higher-GI food sources (P-trend = 0.01), was prospectively related to higher concentrations of IL-6 in younger adulthood, independently of baseline BMI and early life, socioeconomic, and other nutritional factors. Furthermore, a higher dietary GL (P-trend = 0.002) and a lower intake of whole grains (P-trend = 0.01) were independently associated with higher IL-6 concentrations in adults. Dietary GI and added sugar and fiber intakes were not independently associated with IL-6 (P-trend ≥ 0.09). Carbohydrate nutrition during puberty was not independently related to hs-CRP, IL-18, and adiponectin concentrations (all P-trend > 0.1). During puberty, a higher intake of carbohydrates from higher-GI food sources and lower whole-grain consumption prospectively predict greater IL-6 concentrations in young adulthood. These data support the hypothesis that diet during

  15. Relationship Between Changes in Leaf Endogenous Hormone Contents and Senescence During Grain Filling Stage of a Rice Hybrid and its Parents

    Institute of Scientific and Technical Information of China (English)

    FAN Jin-juan; LI Xue-mei; XU Zheng-jin; ZHANG Li-jun

    2004-01-01

    A major problem in hybrid rice production is the occurrence of leaf senescence during the grain filling stage that can result in reduction of yield. Changes in contents of several endogenous hormones are related to leaf senescence. The relationship between endogenous hormones and leaf senescence in the rice hybrid Tiyou 418 and its parents Tijin and C418, was undertaken for investigation. Indicators of leaf senescence, including superoxide dismutase (SOD) activity, malondialdehyde (MDA) accumulation and chlorophyll content, as well as the contents of abscisic acid (ABA), zeatin riboside (ZR), gibberellin (GA1/3) and auxin (IAA) in the leaves were determined. Different rates of leaf senescence were observed in the three materials. Senescence occurred earliest and fastest in Tijin, followed by Tiyou 418 and then C418. A similar trend was recorded in ABA, ZR, and IAA contents during the grain filling stage in the three materials. Changes in (GA1/3+ZR+IAA)/ABA ratios were also similar, being quite stable during the early stage of leaf senescence, and decreasing markedly during the late stage. The ratio declined more dramatically in Tijin, in accordance with its faster leaf senescence. The results suggest that the ratio of (GA1/3+ZR+IAA)/ABA regulates chlorophyll content, SOD activity, MDA content and membrane lipid peroxidation. It is postulated that endogenous hormones may play a role in the regulation of leaf senescence in a systematic way.

  16. Transcriptional coordination and abscisic acid mediated regulation of sucrose transport and sucrose-to-starch metabolism related genes during grain filling in wheat (Triticum aestivum L.).

    Science.gov (United States)

    Mukherjee, Shalini; Liu, Aihua; Deol, Kirandeep K; Kulichikhin, Konstanin; Stasolla, Claudio; Brûlé-Babel, Anita; Ayele, Belay T

    2015-11-01

    Combining physiological, molecular and biochemical approaches, this study investigated the transcriptional coordination and abscisic acid (ABA) mediated regulation of genes involved in sucrose import and its conversion to starch during grain filling in wheat. Sucrose import appears to be mediated by seed localized TaSUT1, mainly TaSUT1D, while sucrose cleavage by TaSuSy2. Temporal overlapping of the transcriptional activation of AGPL1 and AGPS1a that encode AGPase with that of the above genes suggests their significance in the synthesis of ADP-glucose; TaAGPL1A and TaAGPL1D contributing the majority of AGPL1 transcripts. ABA induced repressions of TaSUT1, TaSuSy2, TaAGPL1 and TaAGPS1a imply that ABA negatively regulates sucrose import into the endosperm and its subsequent metabolism to ADP-glucose, the substrate for starch synthesis. The formations of amyloses and amylopectin from ADP-glucose appear to be mediated by specific members of GBSS, and SS, SBE and DBE gene families, and the ABA-induced transcriptional change in most of these genes implies that ABA regulates amylose and amylopectin synthesis. The findings provide insights into the molecular mechanisms underlying the coordination and ABA mediated regulation of sucrose transport into the developing endosperm and its subsequent metabolism to starch during grain filling in wheat.

  17. Changes in the Activities of Enzymes Involved in Starch Synthesis in the Kernel During Grain Filling in Winter Wheat Cultivars of Different Spike Types

    Institute of Scientific and Technical Information of China (English)

    GAO Song-jie; WANG Wen-jing; GUO Tian-cai; HAN Jin-feng

    2003-01-01

    Two winter wheat(Triticum aestivum L. ) cultivars, large-spike type Yumai66 amd small-spike type Yumai49, were used to study the activities of enzymes involved in starch synthesis in the kernel during grain filling. Starch accumulated faster in the kernel of Yumai49 than Yumai66 up to 25 d after anthesis,thereafter starch accumulated faster in the kernel of Yumai66. Starch accumulation in Yumai66 peaked at 20 -25 d after anthesis, while in Yumai49 starch accumulation peaked at 15 -20 d after anthesis and 25 -30 d after anthesis. The first peak was much higher than that of the second. Sucrose content and sucrose synthase activity peaked at 20 and 15 d after anthesis in Yumai66 and Yumai49, respectively. The sucrose content and sucrose synthase activity in Yumai66 were higher than that in Yunai49 during grain filling. ADP-glucose pyrophosphorylase and starch branching enzyme activity in the kernel of Yumai66 peaked at 20 d after anthesis,while soluble starch synthase activity peaked at 10 and 20 d after anthesis. The second peak was much higher than that of the first.

  18. Effects of High Temperature on Antioxidant Enzymes and Lipid Peroxidation in Flag Leaves of Wheat During Grain Filling Period

    Institute of Scientific and Technical Information of China (English)

    LIU Ping; GUO Wen-shan; PU Han-chun; FENG Chao-nian; ZHU Xin-kai; PENG Yong-xin

    2006-01-01

    On the basis of the phytotron, the effects of high temperature (daily average temperature 25, 30, 35 and 40℃, respectively) on antioxidant enzymes and lipid peroxidation in flag leaves of wheat at 50% relative air moisture during grain fastest fillingstage [19-21 days after anthesis (DAA)] were studied. The wheat cultivars tested were Yangmai 9 with weak-gluten and Yangmai 12 with medium-gluten. Compared with 25℃, the higher the temperature was, the higher was the MDA content in flag leaves, while lower were the SOD, POD, and CAT activities. SOD and CAT activities in Yangmai 12 appeared to be more sensitive to high temperature than that in Yangmai 9. But POD activity in Yangmai 12 was less sensitive to high temperature. MDA content in Yangmai 12 was higher than that in Yangmai 9. The 1 000-grain weight declined with increase in temperature.

  19. Synthesis of Salt Soluble Proteins in Barley. Pulse-Labeling Study of Grain Filling in Liquid-Cultured Detached Spikes

    DEFF Research Database (Denmark)

    Giese, Nanna Henriette; Hejgaard, Jørn

    1984-01-01

    The accumulation of salt-soluble proteins in the endosperm of developing barley (Hordeum vulgare L.) grains was examined. Detached spikes of barley were cultured at different levels of nitrogen nutrition and pulse-labeled with [14C] sucrose at specific times after anthesis. Proteins were extracted...... to increased nitrogen nutrition. Two major components, β-amylase and protein Z in particular, had a synthesis profile almost identical to that of the endosperm storage protein, hordein....

  20. Counting carbohydrates

    Science.gov (United States)

    Carb counting; Carbohydrate-controlled diet; Diabetic diet; Diabetes-counting carbohydrates ... Many foods contain carbohydrates (carbs), including: Fruit and fruit juice Cereal, bread, pasta, and rice Milk and milk products, soy milk Beans, legumes, ...

  1. Effect of Temperature on Rice Starch Biosynthesis Metabolism at Grain-Filling Stage of Early Indica Rice

    Institute of Scientific and Technical Information of China (English)

    CHENG Fang-min; ZHU Hai-jiang; ZHONG Lian-jin; SUN Zong-xiu

    2003-01-01

    The sucrose content, starch content and the ratio of amylose to total starch and the activitiesof six key enzymes, sucrose synthase (SS), soluble starch synthesis enzyme (SSS), ADPG pyrophosphorylase(ADPG-Ppase), granule-boundstarch synthase ( GBSS), starch branching enzyme (SBE) and debranchingenzyme (DBE), which involved in starch synthesis metabolism of developing rice grains and effect of tempe-rature on their activities were analyzed by using two early indica varieties Zhefu49 and Jiazao935 in phyto-trons, the daily average temperature was 22 and 32℃ , respectively. Results showed that the sucrose con-tent, the starch content, and the activities of enzymes such as SS, ADPG-Ppase, SSS, SBE, and DBEwere generally higher under the high temperature treatment (32℃)at the beginning stage as compared withthat under low temperature treatment (22℃). However, at the middle and late stages, there were greatdifferences in the change trend of some enzyme activities, and the association with the sucrose content andstarch content was very complicated. It could be found that the activities of SS and SSS under high temper-ature were always lower than that under Iow temperature, which was closely related to the variation of thesucrose content and starch-accumulating rate. Moreover, compared with ADPG-Ppase, the SS and SSS ac-tivities were more sensitive to temperature, indicating that SS and SSS were controlled by enzyme throughaffecting starch accumulating rate. Difference of the amylose/total starch ratio in developing grains between32 and 22℃ was significant and the lower amylose/total starch ratio was always lower in 32℃ , which wasclosely related to the lower activity level of GBSS. Therefore, it could be concluded that effect of tempera-ture on amylose/total starch was more attributable to GBSS rather than to SS, SSS, ADPG-Ppase, SBEand DBE.

  2. Photosynthetic Characteristics and Antioxidative Metabolism of Flag Leaves in Responses to Nitrogen Application During Grain Filling of Field-Grown Wheat

    Institute of Scientific and Technical Information of China (English)

    CAI Rui-guo; WANG Zhen-lin; ZHANG Min; YIN Yan-ping; WANG Ping; ZHANG Ti-bin; GU Feng; DAI Zhong-min; LIANG Tai-bo; WU Yun-hai

    2008-01-01

    A two-factorial experiment was conducted with two wheat cultivars,SN1391(large spike and large grain)and GC8901(multiple spike and medium grain),and two nitrogen(N)application rates(12 and 24 g N m-2),to investigate the responses of photosynthetic characteristics and antioxidative metabolism to nitrogen rates in flag leaves of field-grown wheat during grain filling.The results showed that the content of N and chlorophyll(Chl)in wheat flag leaves decreased after anthesis and the net photosynthetic rate(Pn),effective quantum yield of PS II(ΦPS Ⅱ),efficiency of excitation capture by open PS H reaction centers(Fv'/Fm'),and photochemical quenching coefficient(qp)began to decrease at 14 days after anthesis.However,the maximal efficiency of PS II photochemistry(Fv/Fm)decreased slightly until the late period of senescence and the nonphotochemical quenching coefficient(NPQ)increased during flag leaves' senescence.As a result,a conflict came into being between absorption and utilization to light energy in flag leaves during senescence,which might accelerate the senescence of flag leaves.Compared with GC8901,the lower plant population of SN1391during grain filling was helpful to maintain the higher content of photosynthetic pigment,activity of PS U,and Pn in flag leaves during senescence.The delayed decrease in antioxidative enzyme activity and the lower degree of membrane lipid peroxidation in the senescing leaves of SN1391 were beneficial to protect the photosynthetic apparatus,which lead to the prolonged duration of CO2 assimilation.With the increase of N application,the Chl content of SN1391 flag leaves and the efficiency of excitation captured by open PS II centers increased.At the same time,the thermal dissipation in SN1391 flag leaves at high N(HN)treatment decreased and ?PsⅡimproved greatly,which were favorable to the increase of Pn.The SOD,POD,CAT and APX activities in the flag leaves of SN1391 increased markedly at HN treatment,indicating that these enzymes

  3. Relation Between Frost-Resistance of Winter Grains, Their Respiration Rate and Water – Soluble Carbohydrates Content in Autumn - Spring Period

    Directory of Open Access Journals (Sweden)

    Pomortsev A.V.

    2013-11-01

    Full Text Available The content of water-soluble carbohydrates and respiration rate in the crown tissue of winter wheat, rye and triticale in autumn – winter – spring were studied. In the period and of winter significant differences were revealed between winter crops in the rate of respiration and content of carbohydrates. Respiration of wheat in mid-March increased over February to 33%, and the content of carbohydrates during this period decreased by 10%. Despite the increase in environment temperature by mid-March of winter rye and triticale showed not increase, but rather decrease in the rate of respiration. A higher level of plant resistance of winter rye and triticale to low temperatures, as compared to winter wheat is associated with carbohydrate status and higher stability of respiration process in winter rye and triticale in response to temperature rise in end of winter.

  4. Relationship Between Grain-filling Characteristics and Grain Weight in Different Kernel Positions of Maize%玉米不同部位子粒灌浆特性与粒重的关系研究

    Institute of Scientific and Technical Information of China (English)

    孟佳佳; 董树亭; 张吉旺; 刘鹏; 赵斌

    2013-01-01

      以郑单958(ZD958)和登海661(DH661)为试验材料,研究在4.5万、7.5万、10.5万株/hm2种植密度下玉米上部及中下部子粒灌浆特性和粒重的关系。结果表明,穗粒数和千粒重是决定子粒最终产量的重要因素,两者均随着密度的增加而显著降低。同一密度下,穗位层光照自开花当天(0 d)先降低后升高,总体呈“高-低-高”的变化趋势,底层光照则持续升高。上部子粒的起始生长势、灌浆速率、胚乳细胞数及淀粉含量均低于中下部子粒。相关及通径分析表明,影响粒重的主要因素是灌浆速率、胚乳细胞数目和淀粉含量,其相关系数分别为0.95966**、0.98191**和0.87776*。光照(包括穗位层和底层)主要通过灌浆速率和淀粉含量来间接影响粒重。%Two cultivars ZD958 and DH661 were used to analyze the effects of different planting densities on grain filling characteristics and grain weight in apical and mid-basal kernel positions of maize. The results showed that both grain number and kernel weight decreased with the increase of density and they determined the grain yield to-gether. At the same density, ear layer transmission declined since pollination(0 d) and then increased slowly while that of bottom kept increasing since pollination. The initial division power(R0), division rate, endosperm cell number and starch content of apical kernels were lower than these of mid-basal kernels. Correlation and path analysis showed that main factors that affected grain weight were division rate, endosperm number and starch content, their correlation co-efficients were 0.959 66**, 0.981 91**and 0.877 76*, respectively. Light transmission, including ear layer and bot-tom, affected grain weight via division rate and starch content.

  5. Effect of Exogenous Phytohormone Compounds on Grain -filling of Early Rice%外源激素复配剂对早稻籽粒灌浆特性的影响

    Institute of Scientific and Technical Information of China (English)

    张文学; 管珊红; 孙刚; 胡水秀; 彭春瑞; 李祖章; 刘光荣

    2012-01-01

    在早稻始穗期及齐穗后一周内叶面喷施4种外源激素复配剂,研究了它们对早稻产量、强弱势粒中蔗糖、淀粉积累的影响,并用Richards方程对不同粒位的灌浆过程进行统计回归分析.结果表明:4种复配剂均提高了籽粒灌浆速率,促进了蔗糖转化、淀粉积累,提高了结实率、充实度和产量.与对照相比,喷施外源激素使强、弱势粒的生长终值量分别提高1.77%~2.52%和8.03% ~22.49%;强、弱势粒的起始生长势分别提高0.29% ~6.56%和3.74% ~26.06%;强、弱势粒的最大灌浆速率分别提高5.86%~16.12%和4.63%~27.38%;强、弱势粒的平均灌浆速率分别提高2.78%~17.64%和6.08% ~18.12%.外源激素对弱势粒的灌浆特性影响较强势粒大.4种复配剂对早稻的增产效果为GA3+6- BA+ BR> GA3+ BR>6 - BA+ BR> GA3 +6 - BA,其中,GA3+6- BA+ BR增产高达9.14%.%To clarify the effects of 4 kinds of exogenous phytohormone compounds (PCs) on the yield, sucrose and amylum accumulation in grain of early rice, an experiment was conducted by spraying exogenous PCs at the initial heading stage and full heading stage, and the experimental data were simulated and analyzed with Richards equation. The results indicated that spraying 4 kinds of exogenous PCs all enhanced the grain - filling rate, accelerated the transformation of sucrose and accumulation of amylum, increased seed setting rate, grain plumpness and yield. As compared with the control, the treatments of spraying PCs enhanced the growth capacity of superior grains and inferior grains by 1.77% ~ 2.52% and 8.03% ~ 22.49% respectively, the initial grain - filling potential of superior grains and inferior grains by 0.29% ~ 6.56% and 3.74% ~ 26.06% respectively, the maximum grain - filling rate of superior grains and inferior grains by 5. 86% ~ 16. 12% and 4. 63% ~ 27. 38% separately, and the mean grain - filling rate of superior grains

  6. Sedimentology, architecture, and depositional evolution of a coarse-grained submarine canyon fill from the Gelasian (early Pleistocene) of the Peri-Adriatic basin, Offida, central Italy

    Science.gov (United States)

    Di Celma, Claudio

    2011-07-01

    The early Pleistocene stratigraphic succession of the Peri-Adriatic basin, eastern central Italy, records the filling of an elongate, N-S oriented piggy-back basin located east of the growing Apennine fold-thrust belt. During the Gelasian (2.588-1.806 Ma), large volumes of gravel, sand and mud derived from the emergent Apennines were redistributed into the basin through a number of slope erosional fairways. These sediment conduits are preserved in the rock record as a series of coarse-grained canyon-fill successions that provide an opportunity for assessing, from an outcrop perspective, how this type of deep-water depositional systems evolves and fills. The present study uses measured stratigraphic sections, photopanels, paleocurrent data, careful lithological mapping, and well-log data from a nearby exploration well to constrain the internal organization of one of these canyon fills, referred to herein as the Offida Canyon. A detailed facies analysis suggests that a variety of gravity-driven subaqueous flows were involved in sediment transport and deposition within the submarine canyon, including slumps, cohesive debris flows, and high- and low-density turbidity currents. Five main lithofacies reflecting both canyon-bounding slope deposits and canyon-filling turbidite and debrite depositional elements have been identified within the exposed succession: (i) clast-supported conglomerates (gravel-rich channel complexes); (ii) medium- to thick-bedded sandstones (overbank lobe); (iii) medium- to very thin-bedded sandstones and mudstones (levee-overbank); (iv) pebbly mudstones and chaotic beds (mudstone-rich mass-transport deposits); and (v) massive mudstones (hemipelagic background deposits). These lithofacies are organized in recurring successions and define fining-upward packages that are regarded as the deep-water expression of high-frequency depositional sequences. Each sequence comprises the sedimentary record of major phases of canyon activity and comprises the

  7. Research on Reheat Way Influence to Carbohydrate Digestibility of Grains Steamed Breads%复热方式对杂粮馒头的碳水化合物消化特性影响的研究

    Institute of Scientific and Technical Information of China (English)

    许荣华; 马巧云; 石晶晶; 石艳丽; 苌泓

    2016-01-01

    The effects of reheat way on the carbohydrate digestibility of four kind of steamed breads were studied with an in vitro digestion procedure. The results indicated that carbohydrate hydrolysis process rules were basically the same, direct cooling 30 minutes of hydrolysis process was the fastest, the second was steam reheat after frozen 24 hours, the third was microwave reheat after frozen 24 hours, the fourth was the one in 24 hours at room temperature. Frozen for 24 hours no longer reheat grains steamed bread carbohydrate hydrolysis process was the slowest. Starch digestion of coarse grain food was affected by many factors, needs further study. It could not think that various of grains steamed breads than white steamed buns were more suitable for the diabetics.%运用体外模拟消化方法探讨不同复热方式对4类馒头的碳水化合物消化特性的影响。结果表明,不同杂粮馒头在不同复热方式下,碳水化合物的水解进程规律基本相同。直接冷却30 min的水解进程最快,其次是冷冻24 h蒸汽复热的,第三是冷冻24 h微波复热的,第四是室温放置24 h的。冷冻24 h不复热的杂粮馒头的碳水化合物水解进程最慢。同时杂粮食品的淀粉消化受多种因素的影响,还需进一步研究,不能认为各种杂粮馒头都比白面馒头更适合于糖尿病人食用。

  8. Free Polyamines in Grains in Response to Soil Drought and Their Relationship with Grain Filling of Wheat%小麦籽粒游离多胺对土壤干旱的响应及其与籽粒灌浆的关系

    Institute of Scientific and Technical Information of China (English)

    张伟杨; 徐云姬; 钱希旸; 李银银; 王志琴; 杨建昌

    2016-01-01

    为探明干旱胁迫下小麦内源游离多胺在籽粒灌浆过程中的作用,2013—2014和2014—2015年度选用高产品种扬麦16和宁麦13进行不同水分条件的盆栽试验。自分蘖末期至成熟期设置正常供水(WW)、土壤轻度干旱(MD)和土壤重度干旱(SD)3种处理,观察不同土壤水分对籽粒中游离多胺和籽粒灌浆的影响。2个品种的结果一致表明,与WW相比, MD处理对叶片水势及光合作用没有显著影响,显著增加弱势粒灌浆速率(12.5%)和粒重(11.8%),对强势粒灌浆无显著影响; SD 处理则严重抑制叶片光合作用,显著降低叶片水势,强势粒的灌浆速率和粒重分别下降10.1%和9.5%,弱势粒的灌浆速率和粒重分别下降14.5%和11.7%。MD处理显著提高了灌浆期弱势粒中游离亚精胺(Spd)和精胺(Spm)含量及其与腐胺(Put)的比值,而SD处理的结果则相反。籽粒灌浆速率、粒重与籽粒中Spd和Spm含量及Spd/Put和Spm/Put值呈极显著正相关,与Put含量呈极显著负相关。喷施Spd和Spm,显著增加3个处理弱势粒及 SD 处理强势粒的灌浆速率(11.2%~25.9%)和粒重(9.9%~17.7%),但对 WW 和 MD 处理的强势粒无显著影响;喷施Spd和Spm合成抑制剂[甲基乙二醛-双脒基腙(MGBG)]后,3个处理强、弱势粒的灌浆速率和粒重均显著降低,分别下降20.5%~28.8%和16.9%~28.5%。表明小麦籽粒中多胺对土壤水分的响应因土壤干旱程度而异,通过轻度土壤干旱处理增加籽粒中Spd和Spm含量以及Spd/Put和Spm/Put值,可以促进籽粒灌浆,增加粒重。%For understanding the role of endogenous free polyamines on grain filling of wheat under drought stress, we conducted a two-year pot experiment from September 2013 to June 2015 using high-yield wheat cultivars Yangmai 16 and Ningmai 13 grown in different soil moisture conditions. Three treatments, namely well-watered (WW), moderate soil-drought (MD), and se-vere soil-drought (SD), were imposed from

  9. Effect of A New Foliar Fertilizer on Grain Filling and Yield of Wheat%一种新型植物复合叶肥对小麦灌浆及产量的影响

    Institute of Scientific and Technical Information of China (English)

    李淑能; 史雨刚; 范建荣; 王曙光; 孙黛珍; 闵柯

    2015-01-01

    了解喷施植物复合叶肥"绿荣追施宝"对小麦产量的影响及喷施的最佳时期,为小麦生产上的应用提供参考.以'晋麦84'为试材,小麦起身后不同时期喷施,进而分析籽粒灌浆特性及产量因素.结果表明:在"常规施肥+抽穗期+灌浆期"和"常规施肥+拔节期+抽穗期+灌浆期"喷施"绿荣追施宝"皆能显著延长小麦的灌浆持续时间,获得较大的穗粒数、单株成穗数、千粒重,进而显著提高小麦产量,尤其在拔节期、抽穗期和灌浆期各喷施一次效果更好.建议小麦生产中,除常规施肥外,拔节期、抽穗期、灌浆期各喷施一次"绿荣追施宝",喷施浓度为800倍稀释液.%The aims were to study the effects of"Lurong"on the yield of wheat, determine the optimal spraying period, and provide reference for wheat production. Wheat variety,'Jinmai 84'was sprayed with a new compound foliar fertilizer"Lurong"at different stages after standing, then the characters of grain filling and yield components were analyzed. The results showed"base fertilizer + heading spraying +grain filling spraying"and"base fertilizer +jointing spraying + heading spraying +grain filling spraying"could prolong grain filling, and increase grain number per spike, spike number per plant and thousand kernel weight, further increased wheat yield. Specially, the result of spraying the foliar fertilizer at jointing, heading and grain filling stage individually had the best effect. It was suggested that the foliar fertilizer"Lurong"with 800 times dilution should be sprayed at jointing, heading and grain filling stage in wheat.

  10. Effects of Nano-Zinc oxide and Seed Inoculation by Plant Growth Promoting Rhizobacteria (PGPR on Yield, Yield Components and Grain Filling Period of Soybean (Glycine max L.

    Directory of Open Access Journals (Sweden)

    R. Seyed Sharifi

    2016-02-01

    promoting rhizobacteria application on yield, yield components and grain filling period of soybean. Materials and Methods In order to study the effects of Nano-Zinc oxide and seed inoculation with Brady rhizobium and plant growth promoting rhizobacteria on yield and some agronomic characteristics of soybean, a factorial experiment based on randomized complete block design with three replications was conducted in 2013 at the research farm of the Islamic Azad University, Ardabil Branch. Factors were included foliar application of Nano-Zinc oxide at four levels (Zero as control, 0.3, 0.6 and 0.9 g l-1 and seed inoculation with Brady rhizobium and plant growth promoting rhizobacteria at five levels (without inoculation as control, seed inoculation with Brady rhizobium japanicum, seed inoculation with Brady rhizobium japanicum+Azosprillum lipoferum strain OF, seed inoculation with Brady rhizobium japanicum+Psedomonas putida, seed inoculation with Brady rhizobium japanicum+ Azosprillum lipoferum strain OF+ Psedomonas putida. Results and Discussion The results of growth indices showed that the maximum total dry matter (530 g m-2, crop growth rate (9.48 g.m-2.day-1 and relative growth rate (0.1 g.g-1.day-1 were obtained at foliar application of 0.9 g l-1 Nano-Zinc oxide×seed inoculation with rhizobium+Azosprillum+ Psedomonas and the least of these indices were obtained without of foliar application Nano-Zinc oxide × seed inoculation. The results showed that plant height, the number of nodules per plant, the number of pod per plant, grain yield and grain 100 weight were significantly affected by Nano-Zinc oxide, seed inoculation and interaction of Nano-Zinc oxide×seed inoculation. Maximum of plant height, grain 100 weight, the number of nodules per plant and grain yield were obtained at foliar application of 0.9 g l-1 of Nano-Zinc oxide×seed inoculation with rhizobium and PGPR. Dry weight of nodules per plant, the number of pod per plant and the number of grains per plant

  11. 叶绿素和植酸对杂交稻籽粒灌浆的影响%Effect of Chlorophyll and Phytate on Grain-filling of Hybrid Rice

    Institute of Scientific and Technical Information of China (English)

    丁君辉; 朱菲蓉; 王若仲; 吴俐

    2012-01-01

    625A/226 and 81A/308 which have obvious differences on grain plumpness, were used as experimental materials. The changes of chlorophyll and phytate concent were compared during grain-filling period to study effects of chlorophyll and phytate on grain-filling of hybrid rice. The results showed that both initial chlorophyll content and phytate concent in 81 A/308 were more than these in 625A/226. There were different stimulative effects of chlorophyll and phytate on grain-filling of hybrid rice.%以籽粒充实度差异明显的625A/226和81 A/308为研究材料,通过比较籽粒发育过程中叶绿素和植酸的含量变化,研究两者对杂交稻籽粒灌浆的影响.结果表明:81A/308初始叶绿素含量、植酸含量均高于625A/226,说明叶绿素与植酸对杂交稻籽粒灌浆都有不同程度的促进作用.

  12. Heat stress during seed filling interferes with sulfur restriction on grain composition and seed germination in oilseed rape (Brassica napus L.

    Directory of Open Access Journals (Sweden)

    Sophie eBrunel-Muguet

    2015-04-01

    Full Text Available In coming decades, increasing temperatures are expected to impact crop yield and seed quality. To develop low input systems, the effects of temperature and sulfur (S nutrition in oilseed rape, a high S demanding crop, need to be jointly considered. In this study, we investigated the effects of temperatures (High Temperature (HT, 33 °C/day, 19 °C/night vs. Control Temperature (Ctrl T, 20°C/day, 15°C/day and S supply (High S (HS, 500 µm SO42- vs. Low S (LS, 8.7 µM SO42- during seed filling on (i yield components (seed number, seed dry weight (SDW and seed yield, (ii grain composition (nitrogen (N and S contents and quality (fatty acid (FA composition and seed storage protein (SSP accumulation and (iii germination characteristics (pre-harvest sprouting, germination rates and abnormal seedlings. Abscisic acid (ABA, soluble sugar contents and seed conductivity were also measured. HT and LS decreased the number of seeds per plant. SDW was less affected due to compensatory effects since the number of seeds decreased under stress conditions. While LS had negative effects on seed composition by reducing the FA contents and increasing the ratio S-poor SSPs (12S globulins/S-rich SSPs (2S albumins ratio, HT had positive effects by increasing S and FA contents and decreasing the C18:2/C18:3 ratio and the 12S/2S protein ratio. Seeds produced under HT showed high pre-harvest sprouting rates along with decreased ABA contents and high rates of abnormal seedlings. HT and LS restriction significantly accelerated germination times. High conductivity, which indicates poor seed storage capacity, was higher in HT seeds. Consistently, the lower ratio of (raffinose +stachyose/sucrose in HT seeds indicated low seed storage capacity. We demonstrated the effects of HT and LS on grain and on germination characteristics. These results suggest that hormonal changes might control several seed characteristics simultaneously.

  13. Carbohydrate Analysis

    Science.gov (United States)

    Bemiller, James N.

    Carbohydrates are important in foods as a major source of energy, to impart crucial textural properties, and as dietary fiber which influences physiological processes. Digestible carbohydrates, which are converted into monosaccharides, which are absorbed, provide metabolic energy. Worldwide, carbohydrates account for more than 70% of the caloric value of the human diet. It is recommended that all persons should limit calories from fat (the other significant source) to not more than 30% and that most of the carbohydrate calories should come from starch. Nondigestible polysaccharides (all those other than starch) comprise the major portion of dietary fiber (Sect. 10.5). Carbohydrates also contribute other attributes, including bulk, body, viscosity, stability to emulsions and foams, water-holding capacity, freeze-thaw stability, browning, flavors, aromas, and a range of desirable textures (from crispness to smooth, soft gels). They also provide satiety. Basic carbohydrate structures, chemistry, and terminology can be found in references (1, 2).

  14. Carbohydrate Loading.

    Science.gov (United States)

    Csernus, Marilyn

    Carbohydrate loading is a frequently used technique to improve performance by altering an athlete's diet. The objective is to increase glycogen stored in muscles for use in prolonged strenuous exercise. For two to three days, the athlete consumes a diet that is low in carbohydrates and high in fat and protein while continuing to exercise and…

  15. Carbohydrate microarrays

    DEFF Research Database (Denmark)

    Park, Sungjin; Gildersleeve, Jeffrey C; Blixt, Klas Ola;

    2012-01-01

    In the last decade, carbohydrate microarrays have been core technologies for analyzing carbohydrate-mediated recognition events in a high-throughput fashion. A number of methods have been exploited for immobilizing glycans on the solid surface in a microarray format. This microarray-based technol...

  16. 水稻灌浆期籽粒中3个与淀粉合成有关的酶活性变化%Changes in Activities of Three Enzymes Associated with Star ch Synthesis in Rice Grains during Grain Filling

    Institute of Scientific and Technical Information of China (English)

    杨建昌; 彭少兵; 等

    2001-01-01

    在水培和盆栽条件下,研究了6个水稻品种(含籼/粳杂交组合、新株型品系)灌浆期强、弱势粒中ADPG焦磷酸酶(EC 2.7.7.21)、淀粉合成酶(EC 2.4.1.21)和淀粉分枝酶或Q-酶(EC 2.4.1.18)的活性变化及其与灌浆充实的关系。 3个酶的活性变化与籽粒灌浆动态相关联:淀粉酶最高活性出现的时间稍前或同步于最大灌浆速率的时间; ADPG焦磷酸酶活性峰值的时间在籽粒达最大重量前的3~6d; Q-酶最高活性在籽粒重量接近最大时出现。灌浆期各个酶活性的平均值、最高值以及灌浆前期(花后3~12d)酶的活性与平均灌浆速率、最大灌浆速率、粒重及谷粒充实率均呈显著或极显著的正相关,尤以Q-酶的相关值最大。籼/粳杂交稻组合籽粒中酶的活性的高低与其亲本有关。在抽穗期通过剪叶、疏花或施用氮素等调节灌浆初期的源库关系或植株体内的营养水平,能增加或降低籽粒中特别是弱势粒中酶的活性。这些结果表明:上述3个酶尤其是Q-酶对籽粒灌浆起着重要的调控作用;在育种上注意选择籽粒中酶活性高的亲本,可望培育出籽粒充实好的品种或杂种后代;通过栽培等措施提高灌浆前期籽粒中酶的活性,可提高结实率和粒重。%Six rice cultivars (including 2 japonica/indica hybrid combinations and 1 new plant type line ) were grown in water culture in green house/or in pots in screen house. The activity changes in three enzymes of ADPglusoce pyriphosphorylase (EC 2.7.7. 21), Starch synthase (EC 2.4.1.21) and starch branching enzyme or Q-enzyme (EC 2.4.1.18) in both of superior and inferior grains and the relationship to grain filling were examined. The changes of three enzyme activities were associated with grain filling process. The time reaching a maximum of starch synthase activity was a little earlier than or synchronous with that of grain filling rate. Maximum ADPglucose

  17. Impeded Carbohydrate Metabolism in Rice Plants under Submergence Stress

    Institute of Scientific and Technical Information of China (English)

    Malay Kumar ADAK; Nirmalya GHOSH; Dilip Kumar DASGUPTA; Sudha GUPTA

    2011-01-01

    The detrimental effects of submergence on physiological performances of some rice varieties with special references to carbohydrate metabolisms and their allied enzymes during post-flowering stages have been documented and clarified in the present investigation.It was found that photosynthetic rate and concomitant translocation of sugars into the panicles were both related to the yield.The detrimental effects of the complete submergence were recorded in generation of sucrose,starch,sucrose phosphate synthase and phosphorylase activity in the developing panicles of the plants as compared to those under normal or control (i.e.non-submerged) condition.The accumulation of starch was significantly lower in plants under submergence and that was correlated with ADP-glucose pyrophosphorylase activity.Photosynthetic rate was most affected under submergence in varying days of post-flowering and was also related to the down regulation of Ribulose bisphosphate carboxylase activity.However,under normal or control condition,there recorded a steady maintenance of photosynthetic rate at the post-flowering stages and significantly higher values of Ribulose bisphosphate carboxylase activity.Still,photosynthetic rate of the plants under both control and submerged conditions had hardly any significant correlation with sugar accumulation and other enzymes of carbohydrate metabolism like invertase with grain yield.Finally,plants under submergence suffered significant loss of yield by poor grain filling which was related to impeded carbohydrate metabolism in the tissues.It is evident that loss of yield under submergence is attributed both by lower sink size or sink capacity (number of panicles,in this case) as well as subdued carbohydrate metabolism in plants and its subsequent partitioning into the grains.

  18. Carbohydrate malabsorption

    DEFF Research Database (Denmark)

    Rumessen, J J; Nordgaard-Andersen, I; Gudmand-Høyer, E

    1994-01-01

    Previous studies in small series of healthy adults have suggested that parallel measurement of hydrogen and methane resulting from gut fermentation may improve the precision of quantitative estimates of carbohydrate malabsorption. Systematic, controlled studies of the role of simultaneous hydrogen...

  19. Duração do período de formação do grão em trigo Duration of the grain filling period in wheat

    Directory of Open Access Journals (Sweden)

    E.A. Osório

    1995-08-01

    Full Text Available Admitindo a existência de correlação positiva entre o tempo de enchimento dos grãos e a produtividade, foi estudada a duração do período de formação dos grãos em cultivares brasileiras de trigo. Os resultados obtidos demonstram existir grande variação entre cultivares na duração deste período, o qual mostrou-se menor nas cultivares tardias de ciclo vegetativo longo. Destacaram-se, por possuírem longo período de formação dos grãos, as cultivares super-precoces SB 7519, CEP 7596 e IAS 58; as cultivares precoces Jacui, PF 75171, CNT 9, PEL 72393, Frontana, PAT 7392, IAS 54, Coxilha, Cotiporã e Nobre e as cultivares de ciclo intermediário CNT 8 e PEL 72390. Nenhuma cultivar tardia dentre as testadas evidenciou longo período do espigamento à colheita.Assuming positive correlation between the grain filling period duration and yield this character was studied for 38 Brazilian wheat cultivare. Results demonstrate a great variation in the gram filling period duration that was shorter in late than hi earlier cultivars. The following earlier and semi-late cultivare had the longest grain filling period: SB 7519, CEP 7596, IAS 58, Jacui, PF 75171, CNT 9, PEL 72393, Frontana, PAT 7392, IAS 54, Coxilha, Cotiporã, Nobre CNT 8 and PEL 72390. None of the tested late cultivars has shown a long period from heading to harvest.

  20. Learning about Carbohydrates

    Science.gov (United States)

    ... What Happens in the Operating Room? Learning About Carbohydrates KidsHealth > For Kids > Learning About Carbohydrates A A ... of energy for the body. Two Types of Carbohydrates There are two major types of carbohydrates (or ...

  1. Dynamic Studies on Grain Filling of Japonic Rice under Saline-Sodic Stress%苏打盐碱胁迫下粳稻子粒灌浆动态研究

    Institute of Scientific and Technical Information of China (English)

    李景鹏; 周继全; 王晓丽; 杨福

    2011-01-01

    选用粳稻品种"东稻4号"(DD4)、粳稻品系"G19"作为试验材料,分别种植在pH值为8.01和9.03的苏打盐碱地中.在栽培条件相同的情况下,分析2个供试材料在不同苏打盐碱胁迫下水稻灌浆期干物质积累、子粒灌浆速率和含水量的动态变化.结果表明:重度苏打盐碱胁迫下水稻起始灌浆期都延迟5 d;强势粒干物质积累都明显高于弱势粒,强势粒前期的灌浆速率高于弱势粒,后期呈明显的下降趋势;"DD4"先于"G19"达到干物质积累的高峰,"G19"强势粒灌浆高峰次数多于"DD4";灌浆期"DD4"无论强势粒还是弱势粒均失水较快,而"G19"强势粒失水快,弱势粒相对平缓.%The Japonica rice cultivar DD4 and strain Gl9 were grown under saline-sodic stress on rice paddy with pH 8.01 and 9.03, respectively. The effects of saline-sodic stress on the dynamic changes of grain dry matter accumulation, milking rate and water content during rice milking stage were studied under the same conditions. The results showed that starting of the rice filling delays 5 days under severe saline-sodic stress; The superior grains are superior on dry matter accumulation to inferior grains, strong milking rate during former stage and downtrend later; The peak of dry matter accumulation DD4 is earlier than Gl9, and the times of milking peak of Gl9 superior grains are more than DD4. Water loss of DD4 is faster to both superior and inferior grains, and in this respect, Gl9 superior grains are the same whereas inferior grains exhibit slower water loss comparatively.

  2. Effects of Exogenous Microbial Inoculants on Degradation of Carbohydrate in Spent Grains Compost%外源微生物菌剂对啤酒糟堆肥碳水化合物降解的影响

    Institute of Scientific and Technical Information of China (English)

    喻夜兰; 刘强; 荣湘民; 谢桂先

    2011-01-01

    In order to compost organic waste spent grains to organic fertilizer, the change of carbohydrate content during composting process was studied. The results showed that exogenous microbial inoculants can accelerate degradation rate of total carbon, water-soluble carbon, cellulose, hemicellulose and lignin, reduce the content of total carbon, water-soluble carbon, hemicellulose and lignin. But, the effects of exogenous microbial inoculants on cellulose content were not consistent.%为了研究将有机废弃物啤酒糟堆制成有机肥而变废为宝的过程,探讨了啤酒糟堆制有机肥过程中碳水化合物含量的变化.结果表明:添加外源微生物菌剂有利于促进碳的代谢,有效提高堆体的总碳、水溶性碳、纤维素、半纤维素、木质素的降解率;并能促进堆体的总碳、水溶性碳、半纤维素、木质素的含量下降,但对纤维素含量的影响不一致,既有增强的,也有减弱的.

  3. Contribution of the ear and the flag leaf to grain filling in durum wheat inferred from the carbon isotope signature:Genotypic and growing conditions effects

    Institute of Scientific and Technical Information of China (English)

    Rut Sanchez-Bragado; Abdelhalim Elazab; Bangwei Zhou; Maria Dolors Serret; Jordi Bort; Maria Teresa Nieto-Taladriz; Jos Luis Araus

    2014-01-01

    The ear, together with the flag leaf, is believed to play a major role as a source of assimilates during grain fil ing in C3 cereals. However, the intrusive nature of most of the available methodologies prevents reaching conclusive results in this regard. This study compares the carbon isotope composition (d13C) in its natural abundance in the water-soluble fractions of the flag leaf blade and the ear with the d13C of mature kernels to assess the relative contribution of both organs to grain fil ing in durum wheat (Triticum turgidum L. var. durum). The relative contribution of the ear was higher in landraces compared to modern cultivars, as wel as in response to nitrogen fertilization and water stress. Such genotypic and environmental y driven differences were associated with changes in harvest index (HI), with the relative contribution of the ear being negatively associated with HI. In the case of the genotypic differences, the lower relative contribution of the ear in modern cultivars compared with landraces is probably associated with the appearance in the former of a certain amount of source limitation driven by a higher HI. In fact, the relative contribution of the ear was far more responsive to changes in HI in modern cultivars compared with landraces.

  4. 外源ABA和GA对小麦籽粒内源激素含量及其灌浆进程的影响%Effects of Spraying Exogenous ABA or GA on the Endogenous Hormones Concentration and Filling of Wheat Grains

    Institute of Scientific and Technical Information of China (English)

    杨卫兵; 蔡铁; 倪英丽; 王振林; 尹燕枰; 李文阳; 李勇; 陈晓光; 王平; 陈二影; 郭俊祥

    2011-01-01

    [目的]探讨喷施外源脱落酸(ABA)或赤霉素(GA)对小麦籽粒内源激素含量及其灌浆进程的影响,为激素调控提高籽粒的粒重提供理论依据.[方法]试验选用山农1391和藁城8901两个品种,籽粒灌浆初期喷施ABA或GA两种外源激素于穗部,定期取籽粒样品,用高效液相色谱法测定籽粒内源激素含量,用三次多项方程式对籽粒灌浆进程进行模拟并分析相关参数.[结果]喷施外源ABA或GA均能显著增加两个品种的粒重.外源ABA或GA对粒重的调控存在粒位效应.喷施ABA显著增加强势粒粒重,喷施GA使强势粒和弱势粒粒重均显著增加.喷施外源ABA显著提高籽粒ABA含量,喷施外源GA显著提高籽粒GA含量,喷施外源ABA或外源GA均显著提高灌浆中后期籽粒IAA和CTK的含量,尤其是喷施外源GA效果更为明显.喷施外源ABA或外源GA均能延长籽粒的灌浆持续期,喷施ABA显著提高灌浆初期强势粒的灌浆速率,而喷施GA显著提高灌浆中后期强势粒和弱势粒的灌浆速率.[结论]喷施外源ABA或外源GA通过改变籽粒内源激素水平,调节灌浆起始时间和籽粒灌浆持续期,进而调控籽粒粒重的形成.喷施ABA是通过提高强势粒粒重来增加粒重的,喷施GA是通过提高强势粒和弱势粒的粒重来增加粒重的.%[ Objective ] The objective of the experiment is to explore the relationship between exogenous ABA and GA to the levels of endogenous hormones in grains and the grain filling, and provide a theoretical basis for increasing grain weight under the regulation of exogenous hormone. [Method] The field experiments were carried out at Taian Experimental Station of Shandong Agricultural University, two cultivars Shannong 1391 and Gaocheng 8901 were planted in the farm, exogenous ABA or GA to the spikes were sprayed at initial grain filling stage, spikes were sampled after anthesis till maturity, and grains were partitioned into two groups, superior grains

  5. Experimental study on back filling and rolling compaction of coarse-grained salty soil%粗颗粒盐渍土回填碾压试验研究

    Institute of Scientific and Technical Information of China (English)

    刘志伟; 程东幸; 张希宏

    2012-01-01

    在新疆大规模的开发建设中,大量的工程场地将遇到粗颗粒盐渍土。因厂址区附近建筑材料缺乏,外运距离远,场地回填及地基换填料同时成为工程面临的难题。本文遵循就地取材的原则,开展了粗颗粒盐渍土现场回填碾压试验和专题研究,确定了粗颗粒盐渍土作为回填料的适宜性及相关设计施工参数。试验研究表明:当粗颗粒盐渍土易溶盐含量不是很高,具轻微溶陷性,并不考虑盐胀时,场地料重新回填可消除原有的溶陷性,且地基承载力可达170kPa,通过级配改良,混合料回填碾压后地基承载力可达300kPa,有了较大提高。其成果的获得,具有明显的工程效益。%In the large-scale development and construction in Xinjiang Uygur Autonomous Region, the most engineering site will encounter the coarse-grained salty soil. Due to the lack of building materials near the site and the long-distance transport, the materials for the back filling and soil replacement are facing the engineering problems. Following the principle to obtain raw material locally, the backfill compaction tests with coarse-grained salty soil and the special study in the field are carried out, and the suitability of the coarse-grained salty soil as backfill material and the relative design and construction parameters are determined. When the easy resolving salt content in coarse-grained salty soil is not very high, and with slight thaw collapsibility, without considering the expansion of salts, the experimental study shows that the thaw collapsibility has been eliminated after the back filling and rolling compaction with the site soil, and the bearing capacity can reach 170kPa. Through improving the gradation, the strength of the compacted backfill has been greatly improved; the bearing capacity of the mixed material can reach 300kPa, which obtain the favorable engineering benefit.

  6. Increased Night Temperature Negatively Affects Grain Yield, Biomass and Grain Number in Chilean Quinoa

    Science.gov (United States)

    Lesjak, Jurka; Calderini, Daniel F.

    2017-01-01

    Quinoa high nutritive value increases interest worldwide, especially as a crop that could potentially feature in different cropping systems, however, climate change, particularly rising temperatures, challenges this and other crop species. Currently, only limited knowledge exists regarding the grain yield and other key traits response to higher temperatures of this crop, especially to increased night temperatures. In this context, the main objective of this study was to evaluate the effect of increased night temperature on quinoa yield, grain number, individual grain weight and processes involved in crop growth under the environmental conditions (control treatment) and night thermal increase at two phases: flowering (T1) and grain filling (T2) in southern Chile. A commercial genotype, Regalona, and a quinoa accession (Cod. BO5, N°191, grain bank from Semillas Baer, hereby referred to as Accession) were used, due to their adaptability to Southern Chilean conditions and contrasting grain yield potential, grain weight and size of plants. Temperature was increased ≈4°C above the ambient from 8 pm until 9 am the next morning. Control treatments reached a high grain yield (600 and 397 g m-2, i.e., Regalona and Accession). Temperature increase reduced grain yield by 31% under T1 treatment and 12% when under T2 in Regalona and 23 and 26% in Accession, respectively. Aboveground biomass was negatively affected by the thermal treatments and a positive linear association was found between grain yield and aboveground biomass across treatments. By contrast, the harvest index was unaffected either by genotype, or by thermal treatments. Grain number was significantly affected between treatments and this key trait was linearly associated with grain yield. On the other hand, grain weight showed a narrow range of variation across treatments. Additionally, leaf area index was not affected, but significant differences were found in SPAD values at the end of T1 treatment, compared

  7. Effects of Temperature During the Filling Period on the Physicochemical and Amylogram Properties of Rice Grain%结实期温度对稻米理化特性及淀粉谱特性的影响

    Institute of Scientific and Technical Information of China (English)

    金正勋; 秋太权; 孙艳丽; 金学泳

    2001-01-01

    An experiment was carried out under natural conditions at both high temperature of glass house covered with glass and normal outside temperautre to study the effects of temperature during the filling period on the physicochemical properties and amylogram properties of rice grain. The results showed that with the increasing of the temperature during the filling period, the gelatinization temperature and protein content turned high, the gel consistency grew long, while the amylose content turned low. Correlation analysis showed that daily mean temperature during the filling period has a posritive correlation with gel consistency and protein content, while it has a negative correlation with alkali spreading value and amylose contents. The initial pasting temperature increased with the increasing of temperautre during the filling period, but the changes of peak viscosity, hot viscosity and break down varied from ecotypes of different varieties. Peak viscosity, hot viscosity and break down of early varieties at high-temperature were higher than at normal-temperature, but the peak viscosity, hot viscosity and break down of late varieties at normal temperature is higher than at high temperature.%利用玻璃室内的高温和室外的常温,研究了结实期温度对稻米理化特性和淀粉谱特性的影响。结果表明,随着结实期温度的升高,稻米的糊化温度和蛋白质含量提高,胶稠度变长,而直链淀粉含量下降;相关分析表明,结实期日平均气温与胶稠度和蛋白质含量呈正相关,而与碱消值和直链淀粉含量呈负相关;糊化开始温度随结实期温度的升高而提高,但最高粘度、最低粘度及下降粘度值的变化因品种生态类型不同而异,即在高温条件下结实的稻米与在常温条件下结实的稻米相比,其最高粘度、最低粘度以及下降粘度值,早熟品种前者比后者大,而晚熟品种前者比后者小。

  8. Simulation of Grain-filling of Spring Maize(Zhengdan 958) in Northeast China%东北春玉米郑单958籽粒灌浆过程的模拟∗

    Institute of Scientific and Technical Information of China (English)

    郭春明; 任景全; 曲思邈; 李忠辉

    2015-01-01

    利用白城国家一级农试站2011-2013年春玉米田间试验观测数据,基于 Richards 方程建立和验证东北春玉米籽粒灌浆模型,并进行籽粒灌浆特性分析。结果表明:分别以相对开花后天数、相对活动积温和相对≥10℃有效积温为自变量,相对百粒重为因变量建立的籽粒灌浆过程普适模型(模型 I、模型 II 和模型 III)均通过了0.01水平的显著性检验(R2>0.98)。模型回代后模拟值与实测值的评价指标 NSE(Nash ̄Sutcliffe 指数)和RSR(均方根误差与标准差的比值)值均表现为“很好”的效果。模拟值与实测值散点均在1︰1线±10%范围内。模型 I 显示,东北春玉米籽粒灌浆活跃期为46d,整个灌浆过程平均速率为0.84g.d -1;籽粒灌浆速率在开花后32d 达到最大,为1.23g.d -1,此时玉米百粒重为15.17g;灌浆中期对百粒重的贡献率最大,为65.60%,前期和后期贡献率分别为10.50%和23.90%。模型 II 和模型 III 显示,籽粒灌浆活跃期活动积温和≥10℃有效积温分别为1043.5℃.d 和679.1℃.d;灌浆速率在开花后活动积温和≥10℃有效积温分别为782.8℃.d 和473.3℃.d 时达到最大值;中期灌浆贡献率为67.68%。3个模型均显示,中期灌浆贡献率较大,前期和后期相对较小,说明春玉米籽粒干物质积累主要在灌浆中期完成。%The grain ̄filling model of spring maize was established by richards equation with the experiement data of Baicheng from 2011 to 2013. The grain ̄filling characteristic was analyzed based on the confirm and calibration of the model. The results showed that universal grain ̄filling model passed the 0. 01 level of significance test which the days after flowering(model I), active accumulated temperature(model II), effective accumulated temperature above 10℃(model III) were independent variable and the dry weight of 100 ̄grain was dependent variable. Measured and simulated value scattered

  9. 结实期叶面施锌对扬麦16号和扬辐麦2号籽粒不同部位锌含量的影响%The Effect of Foliar Zn Application at Grain Filling Stage on Zn Content in Grain Fractions of Winter Wheat Yangmai 16 and Yangfumai 2

    Institute of Scientific and Technical Information of China (English)

    齐义涛; 张庆; 周三妮; 杨晓晨; 杨连新; 王云霞

    2013-01-01

    增加谷粒特别是人类食用部位胚乳中的锌浓度是当前生物强化领域一个挑战.2011年在土培条件下,以扬麦16号和扬辐麦2号两个当地高产品种为供试材料,于花后每隔l周喷施0.2%或0.4% ZnSO43次,以喷等量清水为对照,研究结实期叶面喷施锌肥对小麦籽粒不同部位锌含量的影响.结果表明,与对照相比,0.2%、0.4% ZnSO4处理使供试品种籽粒中的锌浓度平均分别增加58%和125%,达极显著水平.利用磨粉机Sedimat Laboratory Mill将小麦籽粒分为麸皮、次粉和面粉3个组分,测定数据表明,锌浓度为麸皮>次粉>面粉,最高和最低浓度相差约8倍.两品种平均,0.2% ZnSO4处理使小麦麸皮、次粉和面粉中锌浓度分别增加64%、54%和37%,0.4% ZnSO4处理使对应部位锌浓度分别增加117%、110%和92%,均达显著或极显著水平.结实期叶面施锌对籽粒各部位锌累积量的影响与锌浓度的响应趋势一致.与此不同,结实期叶面施锌对麸皮、次粉和面粉锌积累量占籽粒总锌积累量的比例没有影响.多数情况下,结实期锌处理对扬麦16号籽粒各部位锌浓度和锌累积量的影响大于扬辐麦2号.以上数据表明,通过选择适宜品种结合叶面施锌,可以经济有效地改善现代小麦品种面粉的锌营养水平.%Zinc (Zn) deficiency is a widespread problem in the world,especially in the developing regions where staple foods derived from cereals are the main source of daily micronutrients intake.Therefore,the increase of Zn content in the endosperm,which is consumed as wheat flour by human being,is the main challenge in the research area of biofortification.Winter wheat cultivar Yangmai 16 and Yangfumai 2,grown in a cement tank filled with clay loam soil,were treated by foliar Zn application at 3 rates of 0 (control),0.2% and 0.4% ZnSO4 at grain filling stage.The first Zn treatment was applied at 1 week after flowering

  10. Effect of Canopy Temperature on Physiological Characteristic and Grain Quality at Filling Stage in Rice%水稻灌浆期冠层温度对植株生理性状及稻米品质的影响

    Institute of Scientific and Technical Information of China (English)

    高继平; 隋阳辉; 张文忠; 姚晨; 高明超; 赵明辉; 徐正进

    2015-01-01

    在大田环境下,以辽粳294、开粳1号为材料,在灌浆期设置5个水分梯度处理,研究了水稻冠层温度日变化特征及其与土壤水分状况、产量生理特性、稻米品质之间的关系.结果表明:1)冠层温度低于气温,但与其显著正相关.梯度水分处理导致冠层温度和冠气温度差逐级升高,即土壤水势降低,冠层温度升高,冠气温度差绝对值增大;2)相同环境条件下,抗旱性弱的品种辽粳294的冠层温度低于抗旱性强的品种开粳1号;3)水分胁迫下水稻冠气温度差与每穗实粒数、千粒重、结实率、产量、整精米率、蛋白质含量、直链淀粉、脂肪酸和食味值呈显著负相关,与秕粒数、垩白度、垩白粒、碎米率呈显著正相关;4)光合速率、气孔导度及蒸腾速率随土壤水势降低而下降,且抗旱性强的品种开粳1号的光合性能较强.相关性分析表明,两个品种冠气温度差与其光合性能显著或极显著负相关;5)开粳1号的气孔密度显著大于辽粳294,而气孔长度和气孔宽度极显著小于辽粳294.综合分析表明,在灌浆期辽粳294和开粳1号在土壤水势为-0.02~-0.03 MP 时,平均冠气温度差分别维持在0.9℃和0.8℃时对产量影响不显著(达到水分临界水平),可作为水稻灌浆期的节水灌溉指标.%A field experiment was conducted to illuminate the daily variation of canopy temperature and its relationship with soil water content,physiological characteristics and grain quality with Liaojing 294 and Kaijing 1 as materials during filling stage at five gradient soil water contents.The results showed that:1 )The canopy temperature,lower than air temperature,was significantly positively correlated with air temperature.The gradient soil water stress led to a gradual increase in the canopy temperature and canopy-air temperature difference.That is to say, the canopy temperature and the absolute value of the canopy

  11. Effects of Irrigation Regimes on Yield and Grain Filling of Rice(Oryza sativaL.) in Cold Region%灌溉方式对寒地水稻产量及籽粒灌浆的影响

    Institute of Scientific and Technical Information of China (English)

    赵黎明; 李明; 郑殿峰; 顾春梅; 那永光; 解保胜

    2015-01-01

    提高;对二次枝梗作用效果大于一次枝梗,其中3粒二次枝梗粒数和产量明显增加,收获指数、千粒重和结实率高,最终籽粒产量高。【结论】灌溉方式对寒地水稻产量形成和籽粒灌浆过程的调控产生较大影响,从增产角度上看,目前轻干湿交替灌溉方式更适合在寒地水稻高产、高效栽培中应用,而重干湿交替和浅水灌溉不利于寒地水稻产量的增加。%[Objective] This paper discusses the influences of irrigation regimes on the tiller dynamics, dry matter accumulation, and grain-filling characteristics of rice in cold regions to deepen our understanding of the regulation of the grain-filling process. This study provides a theoretical basis for guiding the high yield and high-efficiency cultivation of rice.[Method] Kendao 24 and Kongyu 131 (two rice cultivars widely planted in northeastern China) were planted in the field with three types of irrigation regimes, namely, heavy irrigation with alternating wetting and drying (W1), light irrigation with alternating wetting and drying (W2), and irrigation with shallow water (W3). The influences of such irrigation regimes on the yield and grain filling of rice in cold regions were analyzed.[Result]The irrigation treatments caused significant effects on the number of stem and tiller, percentage of earbearing tiller, dry matter accumulation, dry matter transmission in the stem-sheath after heading sprouting, grain-filling properties, and yield and its components. W2 and W1 treatments effectively controlled the ineffective tillering, increased the seed setting rate, reduced the height of the plant, and decreased the lengths of the third and fourth internodes from the top. Under W2 treatment, the percentage of earbearing tiller increased, the dry matter accumulation and transport capability was enhanced, and the 1000-grain weight increased significantly. The filling characteristics showed that the relationship between

  12. Carbohydrates and Diabetes

    Science.gov (United States)

    ... Loss Surgery? A Week of Healthy Breakfasts Shyness Carbohydrates and Diabetes KidsHealth > For Teens > Carbohydrates and Diabetes A A A What's in this ... that you should keep track of how many carbohydrates (carbs) you eat. But what exactly are carbohydrates ...

  13. 不同杂交水稻籽粒灌浆期叶片蛋白的差异表达分析%Analysis of differential expression of leaf proteins in different hybrid rice during grain-filling

    Institute of Scientific and Technical Information of China (English)

    黄锦文; 李忠; 陈军; 张志兴; 李奇松; 郑家团; 林文雄

    2011-01-01

    为明确超级杂交水稻高产形成的分子机理,以超级杂交水稻"Ⅱ优航2号"为试验材料,杂交水稻"汕优63"为对照,运用双向电泳结合质谱技术,比较分析了籽粒灌浆过程中两种不同"源"类型杂交水稻叶片蛋白的差异表达情况.结果显示,两种不同类型水稻叶片蛋白中共有22个蛋白点出现显著差异表达,其中有20个蛋白功能得到鉴定.通过对差异蛋白功能及表达丰度的分析,相对于"汕优63","Ⅱ优航2号"在灌浆期光合代谢、抗逆反应、基因转录表达、细胞生长、能量代谢等生理活动过程中表现出较大优势,是其叶片在籽粒灌浆期"源"优势的主要体现.本研究从差异蛋白水平明确了航天超级杂交稻高产形成的"源"特征,丰富了籽粒灌浆的"源、库、流"理论,为超级杂交水稻育种提供理论依据.%To further clarify molecular metabolism of high yield super-hybrid rice, this paper studied differential expression patterns of leaf proteins in super-hybrid combinations “Ⅱ Youhang No. 2” and hybrid combinations “Shanyou 63” during grain-filling. The proteomic approach, which was based on two-dimensional electrophoresis (2-DE) and mass spectrometry (MS) was used in the study.A total of 22 differentially expressed proteins (at a significant level) were noted, and 20 of which were eventually identified. Further analysis of relative functions and expression abundances showed advantages of “Ⅱ Youhang No. 2” in photosynthetic metabolism,defense, gene-transcript regulation, cell growth, energy metabolism, compared with “Xianyou 63”. The advantages maybe the internal agents of “source” property of “Ⅱ Youhang No. 2” leaf in grain-filling stage. The study revealed the source properties of high yield super-hybrid “Ⅱ Youhang No. 2” at differential proteomic levels. This, to some extent, enriched the source-sink-flow theory and provided a theoretical basis for super-hybrid rice

  14. 超级稻花后强、弱势粒多胺浓度变化及其与籽粒灌浆的关系%Post-Anthesis Changes in Concentrations of Polyamines in Superior and Inferior Spikelets and Their Relation with Grain Filling of Super Rice

    Institute of Scientific and Technical Information of China (English)

    谈桂露; 张耗; 付景; 王志琴; 刘立军; 杨建昌

    2009-01-01

    8 (japonica), were field grown. Concentrations of putrescine (Put), spermidine (Spd) and spermine (Spm) in both inferior and superior spikelets during the grain filling period and grain filling rate were determined. The relationship between polyamine concentrations and grain filling rate were analyzed, and chemical regulators were applied to verify the roles of polyamines in grain filling. The results showed that the maximum grain filling rate, mean grain filling rate, and brown rice weight for superior spikelets showed small difference between the super rice and check rice, but those of inferior spikelets were significantly lower for super rice than for check rice cultivars. Changes in concentrations of polyamines in grains exhibited single peak during the grain filling period. The peak and mean concentrations of Put were greater in inferior than in superior spikelets. The peak and mean concentrations of Spd and Spm were greater in the superior than in the inferior, and greater in the check rice than in the super rice. The mean grain filling rate and brown rice weight were negatively and very significantly correlated with the Put concentrations, and positively and very significantly correlated with Spd and Spm concentrations, Spd/Put, and Spm/Put. Application of Spd or Spm to panicle at the early grain filling stage significantly enhanced activities of sucrose synthetase, adenine di-phosphoglucose pyrophosphorylase, and soluble starch synthetase in inferior spikelets, and significantly increased grain filling rate, seed setting rate and grain weight of inferior spikelets. Application of Put or methylglyoxal-bis (guanylhydrazone) (MGBG, an inhibitor of 5-adenosyl-Z.-methionine decarboxylase) exhibited an opposite effect. The results suggest that polyamines play a role in regulating grain filling. Low concentrations of Spd and Spm and low Spd/Put and Spm/Put may be an important physiological reason for the small grain filling rate and low grain weight of inferior

  15. Carbohydrate Metabolism Disorders

    Science.gov (United States)

    ... you eat. Food is made up of proteins, carbohydrates, and fats. Chemicals in your digestive system (enzymes) ... metabolic disorder, something goes wrong with this process. Carbohydrate metabolism disorders are a group of metabolic disorders. ...

  16. Carbohydrates in Supramolecular Chemistry.

    Science.gov (United States)

    Delbianco, Martina; Bharate, Priya; Varela-Aramburu, Silvia; Seeberger, Peter H

    2016-02-24

    Carbohydrates are involved in a variety of biological processes. The ability of sugars to form a large number of hydrogen bonds has made them important components for supramolecular chemistry. We discuss recent advances in the use of carbohydrates in supramolecular chemistry and reveal that carbohydrates are useful building blocks for the stabilization of complex architectures. Systems are presented according to the scaffold that supports the glyco-conjugate: organic macrocycles, dendrimers, nanomaterials, and polymers are considered. Glyco-conjugates can form host-guest complexes, and can self-assemble by using carbohydrate-carbohydrate interactions and other weak interactions such as π-π interactions. Finally, complex supramolecular architectures based on carbohydrate-protein interactions are discussed.

  17. Carbohydrates as food allergens

    OpenAIRE

    SOH, Jian Yi; Huang, Chiung Hui; Lee, Bee Wah

    2015-01-01

    The literature supports the notion that carbohydrate epitopes, on their own, do not contribute significantly to the induction of allergic reactions. They bind weakly to IgE antibodies and have been termed as cross reactive carbohydrate determinants. These epitopes cause confusion in in vitro IgE testing through nonspecific cross-reactivity. Coincident with the rising trends in food allergy prevalence, there has recently been reports of anaphylaxis induced by carbohydrate epitopes. There are t...

  18. Radioiodinated branched carbohydrates

    Science.gov (United States)

    Goodman, Mark M.; Knapp, Jr., Furn F.

    1989-01-01

    A radioiodinated branched carbohydrate for tissue imaging. Iodine-123 is stabilized in the compound by attaching it to a vinyl functional group that is on the carbohydrate. The compound exhibits good uptake and retention and is promising in the development of radiopharmaceuticals for brain, heart and tumor imaging.

  19. Multimodal CARS microscopy of structured carbohydrate biopolymers

    OpenAIRE

    Slepkov, Aaron D.; Ridsdale, Andrew; Pegoraro, Adrian F.; Moffatt, Douglas J.; Stolow, Albert

    2010-01-01

    We demonstrate the utility of multimodal coherent anti-Stokes Raman scattering (CARS) microscopy for the study of structured condensed carbohydrate systems. Simultaneous second-harmonic generation (SHG) and spectrally-scanned CARS microscopy was used to elucidate structure, alignment, and density in cellulose cotton fibers and in starch grains undergoing rapid heat-moisture swelling. Our results suggest that CARS response of the O-H stretch region (3000 cm−1–3400 cm−1), together with the comm...

  20. Carbohydrates as allergens.

    Science.gov (United States)

    Commins, Scott P

    2015-01-01

    Complex carbohydrates are effective inducers of Th2 responses, and carbohydrate antigens can stimulate the production of glycan-specific antibodies. In instances where the antigen exposure occurs through the skin, the resulting antibody production can contain IgE class antibody. The glycan-stimulated IgE may be non-specific but may also be antigen specific. This review focuses on the production of cross-reactive carbohydrate determinants, the recently identified IgE antibody response to a mammalian oligosaccharide epitope, galactose-alpha-1,3-galactose (alpha-gal), as well as discusses practical implications of carbohydrates in allergy. In addition, the biological effects of carbohydrate antigens are reviewed in setting of receptors and host recognition.

  1. Computerized molecular modeling of carbohydrates

    Science.gov (United States)

    Computerized molecular modleing continues to increase in capability and applicability to carbohydrates. This chapter covers nomenclature and conformational aspects of carbohydrates, perhaps of greater use to carbohydrate-inexperienced computational chemists. Its comments on various methods and studi...

  2. Carbohydrates and Diabetes (For Parents)

    Science.gov (United States)

    ... Old Feeding Your 1- to 2-Year-Old Carbohydrates and Diabetes KidsHealth > For Parents > Carbohydrates and Diabetes ... many kids with diabetes take to stay healthy. Carbohydrates and Blood Sugar The two main forms of ...

  3. Paleovalley fills: Trunk vs. tributary

    Science.gov (United States)

    Kvale, E.P.; Archer, A.W.

    2007-01-01

    A late Mississippian-early Pennsylvanian eustatic sea level drop resulted in a complex lowstand drainage network being eroded across the Illinois Basin in the eastern United States. This drainage system was filled during the early part of the Pennsylvanian. Distinct differences can be recognized between the trunk and tributary paleovalley fills. Fills preserved within the trunk systems tend to be fluvially dominated and consist of bed-load deposits of coarse- to medium-grained sandstone and conglomerate. Conversely, the incised valleys of tributary systems tend to be filled with dark mudstone, thinly interbedded sandstones, and mudstones and siltstones. These finer grained facies exhibit marine influences manifested by tidal rhythmites, certain traces fossils, and macro- and microfauna. Examples of tributary and trunk systems, separated by no more than 7 km (4.3 mi) along strike, exhibit these styles of highly contrasting fills. Useful analogs for understanding this Pennsylvanian system include the Quaternary glacial sluiceways present in the lower Ohio, White, and Wabash river valleys of Indiana (United States) and the modern Amazon River (Brazil). Both the Amazon River and the Quaternary rivers of Indiana have (or had) trunk rivers that are (were) dominated by large quantities of bed load relative to their tributaries. The trunk valley systems of these analogs aggraded much more rapidly than their tributary valleys, which evolved into lakes because depositional rates along the trunk are (were) so high that the mouths of the tributaries have been dammed by bed-load deposits. These Holocene systems illustrate that sediment yields can significantly influence the nature of fill successions within incised valleys independent of rates of sea level changes or proximity to highstand coastlines. Copyright ?? 2007. The American Association of Petroleum Geologists. All rights reserved.

  4. CARBOHYDRATE-CONTAINING COMPOUNDS WHICH BIND TO CARBOHYDRATE BINDING RECEPTORS

    DEFF Research Database (Denmark)

    1995-01-01

    Carbohydrate-containing compounds which contain saccharides or derivatives thereof and which bind to carbohydrate binding receptors are useful in pharmaceutical products for treatment of inflammatory diseases and other diseases.......Carbohydrate-containing compounds which contain saccharides or derivatives thereof and which bind to carbohydrate binding receptors are useful in pharmaceutical products for treatment of inflammatory diseases and other diseases....

  5. Carbohydrates and Depression.

    Science.gov (United States)

    Wurtman, Richard J.; Wurtman, Judith J.

    1989-01-01

    Describes the symptoms, such as appetite change and mood fluctuation, basic mechanisms, and some treatments of Seasonal Affective Disorder (SAD), Carbohydrate-Craving Obesity (CCO) and Premenstrual Syndrome (PMS). Provides several tables and diagrams, and three reading references. (YP)

  6. About Dental Amalgam Fillings

    Science.gov (United States)

    ... Medical Procedures Dental Devices Dental Amalgam About Dental Amalgam Fillings Share Tweet Linkedin Pin it More sharing ... I have my fillings removed? What is dental amalgam? Dental amalgam is a dental filling material used ...

  7. Carbohydrates in therapeutics.

    Science.gov (United States)

    Kilcoyne, Michelle; Joshi, Lokesh

    2007-07-01

    Awareness of the importance of carbohydrates in living systems and medicine is growing due to the increasing understanding of their biological and pharmacological relevance. Carbohydrates are ubiquitous and perform a wide array of biological roles. Carbohydrate-based or -modified therapeutics are used extensively in cardiovascular and hematological treatments ranging from inflammatory diseases and anti-thrombotic treatments to wound healing. Heparin is a well-known and widely used example of a carbohydrate-based drug but will not be discussed as it has been extensively reviewed. We will detail carbohydrate-based and -modified therapeutics, both those that are currently marketed or in various stages of clinical trials and those that are potential therapeutics based on promising preclinical investigations. Carbohydrate-based therapeutics include polysaccharide and oligosaccharide anti-inflammatory, anti-coagulant and anti-thrombotic agents from natural and synthetic sources, some as an alternative to heparin and others which were designed based on known structure-functional relationships. Some of these compounds have multiple biological effects, showing anti-adhesive, anti-HIV and anti-arthrithic activities. Small molecules, derivatives or mimetics of complement inhibitors, are detailed for use in limiting ischemia/ reperfusion injuries. Monosaccharides, both natural and synthetic, have been investigated for their in vivo anti-inflammatory and cardioprotective properties. Modification by glycosylation of natural products, or glycosylation-mimicking modification, has a significant effect on the parent molecule including increased plasma half-life and refining or increasing desired functions. It is hoped that this review will highlight the vast therapeutic potential of these natural bioactive molecules.

  8. Effects of exogenous ABA application on post-anthesis dry matter redistribution and grain starch accumulation of winter wheat with different staygreen characteristics

    Institute of Scientific and Technical Information of China (English)

    Dongqing; Yang; Yongli; Luo; Yingli; Ni; Yanping; Yin; Weibing; Yang; Dianliang; Peng; Zhengyong; Cui; Zhenlin; Wang

    2014-01-01

    The objective of this study was to investigate whether and how exogenous abscisic acid(ABA)is involved in mediating starch accumulation in the grain and redistribution of carbohydrates during grain filling of two wheat cultivars with different staygreen characteristics.At blooming stage,plants of Wennong 6(a staygreen cultivar)and Jimai 20(control)were sprayed with10 mg L-1abscisic acid(ABA)for 3 days.The application of ABA significantly(P<0.05)increased grain filling rate,starch accumulation rate and content,remobilization of dry matters to kernels,and 1000-grain weight of the two cultivars.Exogenous ABA markedly(P<0.05)increased grain yield at maturity,and Wennong 6 and Jiami 20 showed 14.14%and 4.86%higher compared yield than the control.Dry matter accumulation after anthesis of Wennong 6 was also significantly(P<0.05)influenced by exogenous ABA,whereas that of Jimai 20 was unchanged.Application of ABA increased endogenous zeatin riboside(ZR)content 7 days after anthesis(DAA),and spraying ABA significantly increased endogenous indole-3-acetic acid(IAA)and ABA contents from 7 to 21 DAA and decreased gibberellin(GA3)content at 14 DAA,but increased GA3content from 21 to 35 DAA.The results suggested that increased yield of staygreen was due to greater starch assimilation owing to a higher filling rate and longer grain-filling duration.

  9. Carbohydrates, pollinators, and cycads

    Science.gov (United States)

    Marler, Thomas E; Lindström, Anders J

    2015-01-01

    Cycad biology, ecology, and horticulture decisions are not supported by adequate research, and experiments in cycad physiology in particular have been deficient. Our recent report on free sugar content in a range of cycad taxa and tissues sets the stage for developing continued carbohydrate research. Growth and development of cycad pollen, mediation of the herbivory traits of specialist pollinators, and support of expensive strobilus behavioral traits are areas of cycad pollination biology that would benefit from a greater understanding of the role of carbohydrate relations. PMID:26479502

  10. Grains storage using a field technique

    Directory of Open Access Journals (Sweden)

    Gourgue, H.

    1987-01-01

    Full Text Available Maize or other grains can be protected from insects during storage by filling empty drums, and adding a few handfuls of dry laterite soil. The tap is then rescrewed. The drum is ready for storage.

  11. Abscisic Acid and Cytokinin-Induced Osmotic and Antioxidant Regulation in Two Drought-Tolerant and Drought-Sensitive Cultivars of Wheat During Grain Filling Under Water Deficit in Field Conditions

    Directory of Open Access Journals (Sweden)

    Mohammad-Reza SARAFRAZ-ARDAKANI

    2014-09-01

    Full Text Available Phytohormones play critical roles in regulating plant responses to stress. The present study investigates the effect of cytokinin, abscisic acid and cytokinin/abscisic acid interaction on some osmoprotectants and antioxidant parameters induced by drought stress in two wheat cultivars (Triticum aestivum L. of ‘Pishgam’ and ‘MV-17’ as tolerant and sensitive to drought during post-anthesis phase, respectively grown in field conditions. The most considerable effect of the treatments was exhibited 21 days after anthesis. Under drought conditions, the flag leaf soluble carbohydrate content increased in both cultivars while starch content was remarkably decreased in ‘Pishgam’ as compared to ‘MV-17’. Abscisic acid increased total soluble sugar and reduced starch more than other hormonal treatments, although it decreased studied monosaccharaides in ‘Pishgam’, especially. Drought stress induced high proportion of gylycinebetain and free proline in ‘Pishgam’ cultivar. Application of abscisic acid and cytokinin/abscisic acid interaction increased gylycinebetain and proline content in both cultivars under irrigation and drought conditions. The tolerant cultivar exhibited less accumulation of hydrogen peroxide and malondialdehyde in relation to significant increase of catalase and peroxidase activities and α-tocpherol content under drought conditions. All hormonal treatments increased the named enzyme activities under both irrigation and drought conditions, while higher accumulation of α-tocopherol was only showed in case of cytokinin application. Also, abscisic acid and cytokinin/abscisic acid could decrease drought-induced hydrogen peroxide and malondialdehyde level to some extent, although abscisic acid increased both of hydrogen peroxide andmalondialdehyde content in irrigation phase, especially.

  12. Carbohydrates, Sugar, and Your Child

    Science.gov (United States)

    ... Old Feeding Your 1- to 2-Year-Old Carbohydrates, Sugar, and Your Child KidsHealth > For Parents > Carbohydrates, Sugar, ... a 1-ounce equivalent. previous continue Sizing Up Sugar Foods that are high in added sugar (soda, ...

  13. Multimodal CARS microscopy of structured carbohydrate biopolymers

    Science.gov (United States)

    Slepkov, Aaron D.; Ridsdale, Andrew; Pegoraro, Adrian F.; Moffatt, Douglas J.; Stolow, Albert

    2010-01-01

    We demonstrate the utility of multimodal coherent anti-Stokes Raman scattering (CARS) microscopy for the study of structured condensed carbohydrate systems. Simultaneous second-harmonic generation (SHG) and spectrally-scanned CARS microscopy was used to elucidate structure, alignment, and density in cellulose cotton fibers and in starch grains undergoing rapid heat-moisture swelling. Our results suggest that CARS response of the O-H stretch region (3000 cm−1–3400 cm−1), together with the commonly-measured C-H stretch (2750 cm−1–2970 cm−1) and SHG provide potentially important structural information and contrast in these materials. PMID:21258555

  14. Multimodal CARS microscopy of structured carbohydrate biopolymers.

    Science.gov (United States)

    Slepkov, Aaron D; Ridsdale, Andrew; Pegoraro, Adrian F; Moffatt, Douglas J; Stolow, Albert

    2010-11-08

    We demonstrate the utility of multimodal coherent anti-Stokes Raman scattering (CARS) microscopy for the study of structured condensed carbohydrate systems. Simultaneous second-harmonic generation (SHG) and spectrally-scanned CARS microscopy was used to elucidate structure, alignment, and density in cellulose cotton fibers and in starch grains undergoing rapid heat-moisture swelling. Our results suggest that CARS response of the O-H stretch region (3000 cm(-1)-3400 cm(-1)), together with the commonly-measured C-H stretch (2750 cm(-1)-2970 cm(-1)) and SHG provide potentially important structural information and contrast in these materials.

  15. Cyclitols in maturing grains of wheat, triticale and barley

    Directory of Open Access Journals (Sweden)

    Lesław B. Lahuta

    2011-01-01

    Full Text Available In the present study, the feeding of stem-flag leaf-ear explants of wheat, triticale and barley with d-chiro-inositol and d-pinitol was used for modification of the composition of soluble carbohydrates in grains without genetic transformation of plants. Maturing grains indicated ability to uptake exogenously applied cyclitols, not occurring naturally in cereal plants, and synthesized their a-d-galactosides. The pattern of changes in soluble carbohydrates during grain maturation and germination was not disturbed by the uptake and accumulation of cyclitols. Both, d-chiro-inositol and d-pinitol as well as their a-d-galactosides can be an additional pool of soluble carbohydrates accumulated by maturing grains, without decreasing seeds viability. This is the first report indicating the possibility of introduction of cyclitols with potentially human health benefits properties into cereal grains.

  16. Categories used to classify the basin-fill deposits in the Central Valley

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This digital dataset contains the categories used to classify the basin-fill deposits in the Central Valley as either coarse-grained or fine-grained deposits. This...

  17. Specific Carbohydrate Diet: Does It Work?

    Science.gov (United States)

    ... Specific Carbohydrate Diet (SCD) Go Back The Specific Carbohydrate Diet (SCD) Email Print + Share There is no ... diet that has received attention is the Specific Carbohydrate Diet. This diet limits poorly digestible carbohydrates to ...

  18. Whole grains and health: perspective for Asian Indians.

    Science.gov (United States)

    Misra, A; Rastogi, Kavita; Joshi, Shashank R

    2009-02-01

    Grains are a part of human diet for about 10,000 years. Grains are the most important food source of Indian population, due to this carbohydrate consumption constitute approx. 60-70% of total food intake. Variety of grains are available in India, and different grains form staple diets of people in different part of the country. Whole grains are now recognized as an important source of fiber and other nutrients like trace minerals a vitamins. Grains have shown to have a role in prevention and reducing the risk of type 2 diabetes, coronary heart disease, cancer and obesity.

  19. Getting a prescription filled

    Science.gov (United States)

    ... are located inside of a grocery or large "chain" store. It is best to fill all prescriptions ... be used for long-term medicines and medical supplies. The website should have clear directions for filling ...

  20. Seasonal carbohydrate storage and mobilization in bearing and non-bearing pistachio (Pistacia vera) trees.

    Science.gov (United States)

    Spann, Timothy M; Beede, Robert H; Dejong, Theodore M

    2008-02-01

    We analyzed annual carbohydrate storage and mobilization of bearing ("on") and non-bearing ("off") 'Kerman' pistachio (Pistacia vera L.) trees growing on three different rootstocks. On all rootstocks, carbohydrate storage in shoots and branches of "on" and "off" trees was lowest following the spring growth flush. In "off" trees, stored carbohydrates increased and remained high after the initial growth flush. In "on" trees, stem carbohydrates increased temporarily in early summer, but were mobilized in mid-season during kernel fill, and then increased again after nut harvest. During the dormant season, the only substantial differences in carbohydrate storage between previously "on" and "off" trees were found in the roots of the weakest rootstock. The annual carbohydrate storage and mobilization pattern in canopy branches of heavily cropped pistachio trees appeared to be driven by carbohydrate demands related to nut development and untempered by tree vigor. Mobilization of carbohydrates from current-season and 1- and 2-year-old stem wood of "on" trees during the primary period of kernel fill corresponded with the period of inflorescence bud abscission. Thus, the alternate bearing pattern associated with inflorescence bud abscission in 'Kerman' pistachio may be a function of mid-season mobilization of stored carbohydrates in current-season stems resulting in stimulation of inflorescence bud abscission.

  1. Carbohydrates, pollinators, and cycads

    OpenAIRE

    2015-01-01

    Cycad biology, ecology, and horticulture decisions are not supported by adequate research, and experiments in cycad physiology in particular have been deficient. Our recent report on free sugar content in a range of cycad taxa and tissues sets the stage for developing continued carbohydrate research. Growth and development of cycad pollen, mediation of the herbivory traits of specialist pollinators, and support of expensive strobilus behavioral traits are areas of cycad pollination biology th...

  2. Carbohydrate Counting and Diabetes

    Science.gov (United States)

    ... as cakes, cookies, candy, and other desserts juices, soft drinks, fruit drinks, sports drinks, and energy drinks that ... of added sugars for Americans are sugar-sweetened soft drinks, fruit drinks, sports drinks, and energy drinks grain- ...

  3. Effects of Carbohydrate Consumption Case Study: carbohydrates in Bread

    Directory of Open Access Journals (Sweden)

    Neacsu N.A.

    2014-12-01

    Full Text Available Carbohydrates perform numerous roles in living organisms; they are an important source of energy. The body uses carbohydrates to make glucose which is the fuel that gives it energy and helps keep everything going. However, excess carbohydrate consumption has negative health effects. Bread is a basic product in our nutrition and it also is a product with a high content of carbohydrates. So, it is important to find out more information on bread and on the recommended bread type best for consumption.

  4. 小麦中国春 NAM 转录因子 Gpc-1和 Gpc-2灌浆期时空表达模式分析%Spatiotemporal Expression Pattern Analysis of NAM Transcription Factors Gpc-1 and Gpc-2 in Bread Wheat Cultivar Chinese Spring During Grain Filling

    Institute of Scientific and Technical Information of China (English)

    吴丹; 董剑; 要燕杰; 赵万春; 高翔

    2015-01-01

    Objective]The objective of this experiment is to study the roles of no apical meristem (NAM) transcription factors Gpc-1 and Gpc-2 in early senescence and nutrient remobilization to the grain of bread wheat. [Method] Their spatiotemporal expression patterns were investigated during the grain-filling stage in wheat cultivar Chinese Spring. Their temporal expression dynamics were studied in penultimate leaf, flag leaf, peduncle, glume, rachis and the kernel using quantitative real time polymerase chain reaction (qRT-PCR). And the relative expression level was quantified using Pfaffl method with normalization against multiple verified reference genes. Applying mRNA in situ hybridization, the spatial expression pattern was explored in post-anthesis flag leaf, peduncle and the kernel only with digoxin-labeled oligonucleotide probes which were specifically targeting 5′ or 3′ untranslated regions (UTRs) of Gpc-1 and Gpc-2. [Result] Contrary to a previous report, the functional TaNAM-B1 rather than its dysfunctional paralog was found in Chinese Spring, and its nucleotide sequence was identical with the wild-type TtNAM-B1 in T. turgidum var.dicoccoides. All the results showed that Gpc-1 and Gpc-2 were all widely expressed in studied tissues with the exception of the root in which only the transcript of Gpc-1 was detected. The outcomes of mRNA in situ hybridization indicated that all five genes shared cell-type specificities. To be specific, no transcripts were distributed in leaf epidermal cells, pericarp and the seed coat;however, they mainly aggregated in leaf mesophyll cells, aleurone layer, embryo, and the tissues responsible for the mineral element transport (vascular bundle, pigment strand, nucellar projection and the transfer cell) in grain, in which the highest expression level was observed in embryo. In addition, lower expression level was detected in the peduncle and leaf vascular bundle as well. The results of qRT-PCR showed that the temporal expression

  5. Spinodal decomposition in fine grained materials

    Indian Academy of Sciences (India)

    H Ramanarayan; T A Abinandanan

    2003-01-01

    We have used a phase field model to study spinodal decomposition in polycrystalline materials in which the grain size is of the same order of magnitude as the characteristic decomposition wavelength ($\\lambda_{SD}$). In the spirit of phase field models, each grain () in our model has an order parameter ($\\eta_i$) associated with it; $\\eta_i$ has a value of unity inside the th grain, decreases smoothly through the grain boundary region to zero outside the grain. For a symmetric alloy of composition, = 0.5, our results show that microstructural evolution depends largely on the difference in the grain boundary energies, $\\gamma_{gb}$, of A-rich () and B-rich () phases. If $\\gamma^{\\alpha}_{gb}$ is lower, we find that the decomposition process is initiated with an layer being formed at the grain boundary. If the grain size is sufficiently small (about the same as $\\lambda_{SD}$), the interior of the grain is filled with the phase. If the grain size is large (say, about 10 $\\lambda_{SD}$ or greater), the early stage microstructure exhibits an A-rich grain boundary layer followed by a B-rich layer; the grain interior exhibits a spinodally decomposed microstructure, evolving slowly. Further, grain growth is suppressed completely during the decomposition process.

  6. Carbohydrate Microarrays in Plant Science

    DEFF Research Database (Denmark)

    Fangel, Jonatan Ulrik; Pedersen, H.L.; Vidal-Melgosa, S.

    2012-01-01

    industrially and nutritionally. Understanding the biological roles of plant glycans and the effective exploitation of their useful properties requires a detailed understanding of their structures, occurrence, and molecular interactions. Microarray technology has revolutionized the massively high......-throughput analysis of nucleotides, proteins, and increasingly carbohydrates. Using microarrays, the abundance of and interactions between hundreds and thousands of molecules can be assessed simultaneously using very small amounts of analytes. Here we show that carbohydrate microarrays are multifunctional tools...... for plant research and can be used to map glycan populations across large numbers of samples to screen antibodies, carbohydrate binding proteins, and carbohydrate binding modules and to investigate enzyme activities....

  7. Effects of whole grains on coronary heart disease risk.

    Science.gov (United States)

    Harris, Kristina A; Kris-Etherton, Penny M

    2010-11-01

    Characterizing which types of carbohydrates, including whole grains, reduce the risk for coronary heart disease (CHD) is challenging. Whole grains are characterized as being high in resistant carbohydrates as compared with refined grains, meaning they typically are high in fiber, nutrients, and bound antioxidants. Whole grain intake consistently has been associated with improved cardiovascular disease outcomes, but also with healthy lifestyles, in large observational studies. Intervention studies that assess the effects of whole grains on biomarkers for CHD have mixed results. Due to the varying nutrient compositions of different whole grains, each could potentially affect CHD risk via different mechanisms. Whole grains high in viscous fiber (oats, barley) decrease serum low-density lipoprotein cholesterol and blood pressure and improve glucose and insulin responses. Grains high in insoluble fiber (wheat) moderately lower glucose and blood pressure but also have a prebiotic effect. Obesity is inversely related to whole grain intake, but intervention studies with whole grains have not produced weight loss. Visceral fat, however, may be affected favorably. Grain processing improves palatability and can have varying effects on nutrition (e.g., the process of milling and grinding flour increases glucose availability and decreases phytochemical content whereas thermal processing increases available antioxidants). Understanding how individual grains, in both natural and processed states, affect CHD risk can inform nutrition recommendations and policies and ultimately benefit public health.

  8. The role of carbohydrate in dietary prescription for weight loss

    DEFF Research Database (Denmark)

    Astrup, Arne

    to be shown whether a low-glycemic index diet provides benefits beyond this. Low-carbohydrate diets may be an option for inducing weight loss in obese patients, but a very low intake of carbohydrate-rich foods is not commensurate with a healthy and palatable diet in the long term. However, there is evidence......The optimal diet for prevention of weight gain, obesity, metabolic syndrome, and type 2 diabetes is fat-reduced, fibre-rich, high in lowenergy density carbohydrates (fruit, vegetables, and whole grain products), and intake of energy-containing drinks is restricted. The reduction of the total fat...... content of ad libitum diets produces weight loss in both the short-term and over periods as long as 7 years. A fat-reduced diet, combined with physical activity, reduces all risk factors for cardiovascular disease, and reduces the incidence of type 2 diabetes. The combination of reduction of dietary fat...

  9. The Effects of Breakfast Consumption and Composition on Metabolic Wellness with a Focus on Carbohydrate Metabolism.

    Science.gov (United States)

    Maki, Kevin C; Phillips-Eakley, Alyssa K; Smith, Kristen N

    2016-05-01

    Findings from epidemiologic studies indicate that there are associations between breakfast consumption and a lower risk of type 2 diabetes mellitus (T2DM) and metabolic syndrome, prompting interest in the influence of breakfast on carbohydrate metabolism and indicators of T2DM risk. The objective of this review was to summarize the available evidence from randomized controlled trials assessing the impact of breakfast on variables related to carbohydrate metabolism and metabolic wellness. Consuming compared with skipping breakfast appeared to improve glucose and insulin responses throughout the day. Breakfast composition may also be important. Dietary patterns high in rapidly available carbohydrate were associated with elevated T2DM risk. Therefore, partial replacement of rapidly available carbohydrate with other dietary components, such as whole grains and cereal fibers, proteins, and unsaturated fatty acids (UFAs), at breakfast may be a useful strategy for producing favorable metabolic outcomes. Consumption of fermentable and viscous dietary fibers at breakfast lowers glycemia and insulinemia. Fermentable fibers likely act through enhancing insulin sensitivity later in the day, and viscous fibers have an acute effect to slow the rate of carbohydrate absorption. Partially substituting protein for rapidly available carbohydrate enhances satiety and diet-induced thermogenesis, and also favorably affects lipoprotein lipids and blood pressure. Partially substituting UFA for carbohydrate has been associated with improved insulin sensitivity, lipoprotein lipids, and blood pressure. Overall, the available evidence suggests that consuming breakfast foods high in whole grains and cereal fiber, while limiting rapidly available carbohydrate, is a promising strategy for metabolic health promotion.

  10. Study on Yield Loss of Summer Maize Due to Lodging at the Big Flare Stage and Grain Filling Stage%大喇叭口及灌浆期倒伏对夏玉米产量损失的研究

    Institute of Scientific and Technical Information of China (English)

    李树岩; 马玮; 彭记永; 陈忠民

    2015-01-01

    差异不显著,其中FSH(2011年)处理秃尖率最高,达27.4%。倒伏灾害同时影响穗粒数和百粒重,倒伏后均表现出减少趋势,与产量的相关系数分别为0.729和0.842(P<0.01)。受2年试验条件差异影响,2011年各倒伏处理的穗粒数显著低于对照,2012年仅灌浆期茎倒伏与对照差异显著。倒伏影响百粒重,其中灌浆期处理最显著(P<0.05)。穗粒数和百粒重与产量显著相关,相关系数分别为0.729和0.842(P<0.01)。倒伏显著降低产量,除大喇叭口期轻度根倒伏BR1外,其他倒伏处理的产量均显著低于对照(P<0.05)。大喇叭口期倒伏 BR2、BSL 和 BSH 产量损失率2年平均分别为13.9%、27.9%和27.1%;灌浆期倒伏FR1、FR2、FSL和FSH产量损失率,2年平均分别为29.0%、38.4%、45.0%和48.3%。【结论】相同倒伏类型,灌浆期倒伏较大喇叭口期倒伏影响更大;在同一生育时期,茎倒伏比根倒伏影响更明显,但茎倒伏高、低节位处理之间产量损失差异不显著。各倒伏处理的产量损失表现为灌浆期茎倒伏最高,大喇叭口期根倒伏最低。%Objective]The objective of this study was to analyze the effects of lodging on dry matter, yield components and final yield of summer maize, and further to evaluate the yield losses due to different types of lodging at different growing stages.[Method]The field experiments were conducted during two growing seasons of summer maize with the variety of Xundan 20 from 2011 to 2012 at Zhengzhou Agro-meteorological Experiment Station. The lodging was implemented by kicking down the maize plants artificially at two growing stage, the big flare stage (B) and the grain filling stage (F). And at each stage, the lodging included four types, light lodging of roots (R1, in which the angle between the lodged stem and ground was between 30 and 60 degree), serious lodging of roots (R2, in which the angle between the lodged stem and ground was lower

  11. Impact of Dietary Carbohydrate and Protein Levels on Carbohydrate Metabolism

    Science.gov (United States)

    Lasker, Denise Ann

    2009-01-01

    The goal of this dissertation was to investigate the impact of changing dietary carbohydrate (CARB) intakes within recommended dietary guidelines on metabolic outcomes specifically associated with glycemic regulations and carbohydrate metabolism. This research utilized both human and animal studies to examine changes in metabolism across a wide…

  12. Contracts for Grain Biosecurity and Grain Quality

    OpenAIRE

    Abougamos, Hoda; White, Benedict; Sadler,Rohan

    2012-01-01

    The export of grain from Western Australia depends upon a grain supply network that takes grain from farm to port through Cooperative Bulk Handling receival and storage sites. The ability of the network to deliver pest free grain to the port and onto ship depends upon the quality of grain delivered by farmers and the efficacy of phosphine based fumigation in controlling stored grain pests. Phosphine fumigation is critical to the grain supply network because it is the cheapest effective fumiga...

  13. Synthesis of carbohydrate-based surfactants

    Energy Technology Data Exchange (ETDEWEB)

    Pemberton, Jeanne E.; Polt, Robin L.; Maier, Raina M.

    2016-11-22

    The present invention provides carbohydrate-based surfactants and methods for producing the same. Methods for producing carbohydrate-based surfactants include using a glycosylation promoter to link a carbohydrate or its derivative to a hydrophobic compound.

  14. Grain Spectroscopy

    Science.gov (United States)

    Allamandola, L. J.

    1992-01-01

    Our fundamental knowledge of interstellar grain composition has grown substantially during the past two decades thanks to significant advances in two areas: astronomical infrared spectroscopy and laboratory astrophysics. The opening of the mid-infrared, the spectral range from 4000-400 cm(sup -1) (2.5-25 microns), to spectroscopic study has been critical to this progress because spectroscopy in this region reveals more about a materials molecular composition and structure than any other physical property. Infrared spectra which are diagnostic of interstellar grain composition fall into two categories: absorption spectra of the dense and diffuse interstellar media, and emission spectra from UV-Vis rich dusty regions. The former will be presented in some detail, with the latter only very briefly mentioned. This paper summarized what we have learned from these spectra and presents 'doorway' references into the literature. Detailed reviews of many aspects of interstellar dust are given.

  15. Impact of dietary polyphenols on carbohydrate metabolism.

    Science.gov (United States)

    Hanhineva, Kati; Törrönen, Riitta; Bondia-Pons, Isabel; Pekkinen, Jenna; Kolehmainen, Marjukka; Mykkänen, Hannu; Poutanen, Kaisa

    2010-03-31

    Polyphenols, including flavonoids, phenolic acids, proanthocyanidins and resveratrol, are a large and heterogeneous group of phytochemicals in plant-based foods, such as tea, coffee, wine, cocoa, cereal grains, soy, fruits and berries. Growing evidence indicates that various dietary polyphenols may influence carbohydrate metabolism at many levels. In animal models and a limited number of human studies carried out so far, polyphenols and foods or beverages rich in polyphenols have attenuated postprandial glycemic responses and fasting hyperglycemia, and improved acute insulin secretion and insulin sensitivity. The possible mechanisms include inhibition of carbohydrate digestion and glucose absorption in the intestine, stimulation of insulin secretion from the pancreatic beta-cells, modulation of glucose release from the liver, activation of insulin receptors and glucose uptake in the insulin-sensitive tissues, and modulation of intracellular signalling pathways and gene expression. The positive effects of polyphenols on glucose homeostasis observed in a large number of in vitro and animal models are supported by epidemiological evidence on polyphenol-rich diets. To confirm the implications of polyphenol consumption for prevention of insulin resistance, metabolic syndrome and eventually type 2 diabetes, human trials with well-defined diets, controlled study designs and clinically relevant end-points together with holistic approaches e.g., systems biology profiling technologies are needed.

  16. Impact of Dietary Polyphenols on Carbohydrate Metabolism

    Directory of Open Access Journals (Sweden)

    Kati Hanhineva

    2010-03-01

    Full Text Available Polyphenols, including flavonoids, phenolic acids, proanthocyanidins and resveratrol, are a large and heterogeneous group of phytochemicals in plant-based foods, such as tea, coffee, wine, cocoa, cereal grains, soy, fruits and berries. Growing evidence indicates that various dietary polyphenols may influence carbohydrate metabolism at many levels. In animal models and a limited number of human studies carried out so far, polyphenols and foods or beverages rich in polyphenols have attenuated postprandial glycemic responses and fasting hyperglycemia, and improved acute insulin secretion and insulin sensitivity. The possible mechanisms include inhibition of carbohydrate digestion and glucose absorption in the intestine, stimulation of insulin secretion from the pancreatic b-cells, modulation of glucose release from the liver, activation of insulin receptors and glucose uptake in the insulin-sensitive tissues, and modulation of intracellular signalling pathways and gene expression. The positive effects of polyphenols on glucose homeostasis observed in a large number of in vitro and animal models are supported by epidemiological evidence on polyphenol-rich diets. To confirm the implications of polyphenol consumption for prevention of insulin resistance, metabolic syndrome and eventually type 2 diabetes, human trials with well-defined diets, controlled study designs and clinically relevant end-points together with holistic approaches e.g., systems biology profiling technologies are needed.

  17. Filling a Conical Cavity

    Science.gov (United States)

    Nye, Kyle; Eslam-Panah, Azar

    2016-11-01

    Root canal treatment involves the removal of infected tissue inside the tooth's canal system and filling the space with a dense sealing agent to prevent further infection. A good root canal treatment happens when the canals are filled homogeneously and tightly down to the root apex. Such a tooth is able to provide valuable service for an entire lifetime. However, there are some examples of poorly performed root canals where the anterior and posterior routes are not filled completely. Small packets of air can be trapped in narrow access cavities when restoring with resin composites. Such teeth can cause trouble even after many years and lead the conditions like acute bone infection or abscesses. In this study, the filling of dead-end conical cavities with various liquids is reported. The first case studies included conical cavity models with different angles and lengths to visualize the filling process. In this investigation, the rate and completeness at which a variety of liquids fill the cavity were observed to find ideal conditions for the process. Then, a 3D printed model of the scaled representation of a molar with prepared post spaces was used to simulate the root canal treatment. The results of this study can be used to gain a better understanding of the restoration for endodontically treated teeth.

  18. Decarbonylation and dehydrogenation of carbohydrates

    Science.gov (United States)

    Andrews, Mark A.; Klaeren, Stephen A.

    1991-01-01

    Carbohydrates, especially aldose or ketose sugars, including those whose carbonyl group is masked by hemi-acetal or hemi-ketal formation, are decarbonylated by heating the feed carbohydrate together with a transition metal complex in a suitable solvent. Also, primary alcohols, including sugar alditols are simultaneously dehydrogenated and decarbonylated by heating a mixture of rhodium and ruthenium complexes and the alcohol and optionally a hydrogen acceptor in an acceptable solvent. Such defarbonylation and/or dehydrogenation of sugars provides a convenient procedure for the synthesis of certain carbohydrates and may provide a means for the conversion of biomass into useful products.

  19. Carbon partitioning between oil and carbohydrates in developing oat (Avena sativa L.) seeds.

    Science.gov (United States)

    Ekman, Asa; Hayden, Daniel M; Dehesh, Katayoon; Bülow, Leif; Stymne, Sten

    2008-01-01

    Cereals accumulate starch in the endosperm as their major energy reserve in the grain. In most cereals the embryo, scutellum, and aleurone layer are high in oil, but these tissues constitute a very small part of the total seed weight. However, in oat (Avena sativa L.) most of the oil in kernels is deposited in the same endosperm cells that accumulate starch. Thus oat endosperm is a desirable model system to study the metabolic switches responsible for carbon partitioning between oil and starch synthesis. A prerequisite for such investigations is the development of an experimental system for oat that allows for metabolic flux analysis using stable and radioactive isotope labelling. An in vitro liquid culture system, developed for detached oat panicles and optimized to mimic kernel composition during different developmental stages in planta, is presented here. This system was subsequently used in analyses of carbon partitioning between lipids and carbohydrates by the administration of 14C-labelled sucrose to two cultivars having different amounts of kernel oil. The data presented in this study clearly show that a higher amount of oil in the high-oil cultivar compared with the medium-oil cultivar was due to a higher proportion of carbon partitioning into oil during seed filling, predominantly at the earlier stages of kernel development.

  20. Carbohydrate microarrays in plant science.

    Science.gov (United States)

    Fangel, Jonatan U; Pedersen, Henriette L; Vidal-Melgosa, Silvia; Ahl, Louise I; Salmean, Armando Asuncion; Egelund, Jack; Rydahl, Maja Gro; Clausen, Mads H; Willats, William G T

    2012-01-01

    Almost all plant cells are surrounded by glycan-rich cell walls, which form much of the plant body and collectively are the largest source of biomass on earth. Plants use polysaccharides for support, defense, signaling, cell adhesion, and as energy storage, and many plant glycans are also important industrially and nutritionally. Understanding the biological roles of plant glycans and the effective exploitation of their useful properties requires a detailed understanding of their structures, occurrence, and molecular interactions. Microarray technology has revolutionized the massively high-throughput analysis of nucleotides, proteins, and increasingly carbohydrates. Using microarrays, the abundance of and interactions between hundreds and thousands of molecules can be assessed simultaneously using very small amounts of analytes. Here we show that carbohydrate microarrays are multifunctional tools for plant research and can be used to map glycan populations across large numbers of samples to screen antibodies, carbohydrate binding proteins, and carbohydrate binding modules and to investigate enzyme activities.

  1. Transition metals in carbohydrate chemistry

    DEFF Research Database (Denmark)

    Madsen, Robert

    1997-01-01

    This review describes the application of transition metal mediated reactions in carbohydrate synthesis. The different metal mediated transformations are divided into reaction types and illustrated by various examples on monosaccharide derivatives. Carbon-carbon bond forming reactions are further ...

  2. Carbohydrate drugs: current status and development prospect.

    Science.gov (United States)

    Zhang, Yan; Wang, Fengshan

    2015-04-01

    In recent years, there has been a great effort devoted to the investigation of the roles of carbohydrates in various essential biological processes and the development of carbohydrates to therapeutic drugs. This review summarizes the carbohydrate drugs which have been recorded in several pharmacopoeias, marketed, and under development. A prospect of the future development of carbohydrate drugs is discussed as well.

  3. Gender Differences in Carbohydrate Metabolism and Carbohydrate Loading

    Directory of Open Access Journals (Sweden)

    Willoughby Darryn

    2006-06-01

    Full Text Available Abstract Prior to endurance competition, many endurance athletes participate in a carbohydrate loading regimen in order to help delay the onset of fatigue. The "classic" regimen generally includes an intense glycogen depleting training period of approximately two days followed by a glycogen loading period for 3–4 days, ingesting approximately 60–70% of total energy intake as carbohydrates, while the newer method does not consist of an intense glycogen depletion protocol. However, recent evidence has indicated that glycogen loading does not occur in the same manner for males and females, thus affecting performance. The scope of this literature review will include a brief description of the role of estradiol in relation to metabolism and gender differences seen in carbohydrate metabolism and loading.

  4. Carbohydrates Through Animation: Preliminary Step

    Directory of Open Access Journals (Sweden)

    J.K. Sugai

    2004-05-01

    Full Text Available Methods of education are changing, so the educational tools must change too. The developmentof the systems of information and communication gave the opportunity to bring new technology tothe learning process. Modern education needs interactive programs that may be available to theacademic community, in order to ease the learning process and sharing of the knowledge. Then,an educational software on Carbohydrates is being developed using concept maps and FLASH-MXanimations program, and approached through six modules. The introduction of Carbohydrates wasmade by the module Carbohydrates on Nature, which shows the animations gures of a teacher andstudents, visiting a farm, identifying the carbohydrates found in vegetables, animals, and microor-ganisms, integrated by links containing short texts to help understanding the structure and functionof carbohydrates. This module was presented, as pilot experiment, to teachers and students, whichdemonstrated satisfaction, and high receptivity, by using animation and interactivitys program asstrategy to biochemistrys education. The present work is part of the project Biochemistry throughanimation, which is having continuity.

  5. Chemical composition of grains from glyphosate-resistant soybean and its conventional parent under different edaphoclimatic conditions in Brazil

    Directory of Open Access Journals (Sweden)

    Letícia Carlos Babujia

    2015-10-01

    Full Text Available Glyphosate-tolerant (Roundup Ready®, RR soybean is the most widely cultivated genetically modified crop in the world. The aim of this study was to evaluate, in a long-term field experiment, the chemical composition of grains in a pair of cultivars composed of the conventional parent (BRS 133 and the nearly isogenic transgenic (BRS 245RR soybean. Plants were cropped under two different edaphoclimatic conditions in Brazil. The experiments began during the 2003/2004 growing season; samples were collected during the 2011/2012 harvest. Were quantified the contents of isoflavones (high performance liquid chromatography, fatty acids (gas chromatography, lipid and proteins (near infrared spectroscopy in the NIR - Near Infrared Reflectance and minerals (atomic absorption flame spectrophotometry. All samples were analyzed in three replications. Major differences were observed between the two cultivation locations. In Ponta Grossa, which has lower temperatures and higher rainfall during the grain filling period, the contents of lipids and isoflavones were higher. In Londrina, which hassoil with higher fertility, the contents of minerals, proteins and carbohydrates in soybean grains were higher. Significant differences were observed between the cultivars. The conventional parent had higher protein contents and lower lipid contents. The transgenic soybean line had higher isoflavone contents.

  6. Quantitative partition of protein, carbohydrate and fat pools in growing pigs

    DEFF Research Database (Denmark)

    Chwalibog, André; Thorbek, G

    1995-01-01

    A model combining data from balance experiments with data from oxidation of nutrients demonstrating the pools of protein, carbohydrate and fat and their partition in the body was presented. Data from more than 200 experiments with growing pigs were used to fill up the "black boxes" in the model a...

  7. High throughput screening of starch structures using carbohydrate microarrays

    DEFF Research Database (Denmark)

    Tanackovic, Vanja; Rydahl, Maja Gro; Pedersen, Henriette Lodberg

    2016-01-01

    In this study we introduce the starch-recognising carbohydrate binding module family 20 (CBM20) from Aspergillus niger for screening biological variations in starch molecular structure using high throughput carbohydrate microarray technology. Defined linear, branched and phosphorylated...... maltooligosaccharides, pure starch samples including a variety of different structures with variations in the amylopectin branching pattern, amylose content and phosphate content, enzymatically modified starches and glycogen were included. Using this technique, different important structures, including amylose content...... and branching degrees could be differentiated in a high throughput fashion. The screening method was validated using transgenic barley grain analysed during development and subjected to germination. Typically, extreme branching or linearity were detected less than normal starch structures. The method offers...

  8. Microstructure Filled Hohlraums

    Energy Technology Data Exchange (ETDEWEB)

    Moore, A. S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Thomas, C. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Reese, T. M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2017-02-24

    We propose replacing the gas fill in a hohlraum with a low average density, variable uniformity 3D printed structure. This creates a bimodal hohlraum which acts like a vacuum hohlraum initially during the picket, but could protect the capsule from glint or direct illumination, and then once expanded, homogenizes to behave like a variable z gas-fill during peak portion of the drive. This is motivated by a two main aims: 1) reduction of the Au bubble velocity to improve inner beam propagation, and 2) the introduction of a low density, high-Z, x-ray converter to improve x-ray production in the hohlraum and uniformity of the radiation field seen by the capsule.

  9. Challenges with nonfiber carbohydrate methods.

    Science.gov (United States)

    Hall, M B

    2003-12-01

    Nonfiber carbohydrates (NFC) encompass a compositionally and nutritionally diverse group exclusive of those carbohydrates found in NDF. Their content in feeds has often been described as a single value estimated by difference as 100% of dry matter minus the percentages of CP, NDF (adjusted for CP in NDF), ether extract, and ash. A calculated value was used because of difficulties with assays for individual NFC, but it does not differentiate among nutritionally distinct NFC. Errors in NFC estimation can arise from not accounting for CP in NDF and when multipliers other than 6.25 are appropriate to estimate CP. Analyses that begin to distinguish among NFC are those for starch, soluble fiber (non-NDF, nonstarch polysaccharides), and low molecular weight carbohydrates (mono- and oligosaccharides). Many starch analyses quantify alpha-glucans through specific hydrolysis of alpha-(1 --> 4) and alpha-(1 --> 6) linkages in the glucan, and measurement of released glucose. Incomplete gelatinization and hydrolysis will lead to underestimation of starch content. Starch values are inflated by enzyme preparations that hydrolyze carbohydrates other than alpha-glucan, measurement of all released monosaccharides without specificity for glucose, and failure to exclude free glucose present in the unhydrolyzed sample. Soluble fiber analyses err in a fashion similar to NFC if estimation of CP requires multipliers other than 6.25, or if contaminants such as CP and starch have not been properly accounted. Depolymerization and incomplete precipitation can also decrease soluble fiber estimates. The low molecular weight carbohydrates have been defined as carbohydrates soluble in 78 to 80% ethanol, which separates them from polysaccharides. They can be measured in extracts using broad-spectrum colorimetric assays (phenol-sulfuric acid assay or reducing sugar analysis of acid hydrolyzed samples) or chromatographic methods. Limitations of the colorimetric assays include lack of differentiation

  10. 10 Tips for Parents: How to Get Kids to Eat More Complex Carbohydrates.

    Science.gov (United States)

    PTA Today, 1991

    1991-01-01

    Offers 10 suggestions to help parents encourage their children to eat more complex carbohydrates. Suggestions include microwaving baked potatoes; baking whole-grain breads; snacking on gingersnaps, fig bars, pretzels, or vegetable sticks; mixing cereal into low-fat yogurt; and making soup containing beans, rice, pasta, or potatoes. (SM)

  11. Impact of mammalian enzyme pretreatment of the fermentability of carbohydrate-rich feedstuffs

    NARCIS (Netherlands)

    Bauer, E.; Williams, B.A.; Voigt, C.; Mosenthin, R.; Verstegen, M.W.A.

    2003-01-01

    Several carbohydrate-rich substrates (spent brewer's grains, potato peel, potato starch, wheat bran, sugarbeet pulp and a maize-based standard diet for pigs) were pretreated with digestive enzymes, and the in vitro fermentability of these treated substrates and their untreated counterparts was asses

  12. 灌浆结实期高温干旱复合胁迫对稻米直链淀粉及蛋白质含量的影响%Effects of combined high temperature and drought stress on amylose and protein contents at rice grain-filling stage

    Institute of Scientific and Technical Information of China (English)

    高焕晔; 王三根; 宗学凤; 腾中华; 赵芳明; 刘照

    2012-01-01

    Grain-filling stage is a critical stage for rice yield and quality.There have been frequent high temperatures and droughts in several rice production regions in South China due mainly to global climate change but also to local topographic conditions.This has resulted in simultaneous decline in rice yield and quality.This study investigated the combined effects of high temperature and drought stress on rice quality at grain-filling stage via analyzing the changes in amylose and protein contents of indica rice (intro-gression line of H5 with induced genes of Alternanthera hiloxeroides).A pot experiment was conducted in greenhouse conditionswith experimental treatments including optimal temperature plus moderate drought (OT-MD),optimal temperature plus severe drought (OT-SD),high temperature plus well-watered condition (HT-WW),high temperature plus moderate drought (HT-MD),high temperature plus severe drought (HT-SD) and then optimal temperature plus well-watered condition (OT-WW) as the control treatment (CK).In addition to CK,treatment durations were set at 10 days,20 days,30 days and 40 days,respectively.The results showed that while amylose content decreased,that of protein increased during high temperature period.Compared with CK,amylose content dropped by 1.783%~5.987% while that of protein increased by 1.178%~2.741% during 10~40 days heat period.A similartrend was noted for drought conditions.In other words,amylose content decreased while protein content increased during drought.Compared with CK,amylose content decreased by 1.956%~6.270% while protein content increased by 1.153%~2.944% under 10~40 days of OT-MD treatment.Stress-driven changes in amylose and protein contents were higher under OT-SD treatment than under OT-MD treatment.While combined drought and high temperature stress reduced amylose content,it enhanced protein content.The ranges for amylose and protein contents of rice due to combined high temperature and drought stress were higher

  13. Grain growth and experimental deformation of fine-grained ice aggregates

    Science.gov (United States)

    Diebold, Sabrina; de Bresser, Hans; Spiers, Chris; Durham, William B.; Stern, Laura

    2010-05-01

    Ice is one of the most abundant materials in our solar system. It is the principal constituent of most of the moons of the outer solar system. Thus, the flow behavior of ice is of great interest when studying geodynamic processes on icy moons. Grain growth is an elementary process that is assumed to be important in the ice sheet layering of planetary moons, where temperatures 100-273 K exist. We concentrate on the questions to what extent grain growth may influence the evolution of strength of deforming ice and if the grain growth process is independent or dependent of deformation. The answers to these questions will help us to quantitatively test the hypothesis that the progressive evolution of the grain (crystal) size distribution of deforming and recrystallizing ice directly affects its rheological behaviour in terms of composite grain-size-sensitive (GSS) and grain-size-insensitive (GSI) creep, and that this might, after time, result in a steady state balance between mechanisms of GSS and GSI creep. We performed static grain growth experiments at different temperatures and a pressure (P) of 1 atm, and deformation experiments at P = 30-100 MPa starting in the GSS-creep field. The starting material ice Ih has a grain size growth tests a Hart Scientific temperature bath was filled with d-Limonene as cooling medium. The ice specimens were put into sealed alumina cylinders. For the grain growth tests, temperatures (T) between 213 K and 268 K were chosen. The durations of these tests varied between one day and two weeks. For the deformation experiments, temperatures of > 170 K and strain rates between 10-8 s-1 and 10-4 s-1 were chosen. Grain sizes, grain size distributions and grain topologies were measured by cryogenic SEM and image analysis techniques. We found clear evidence of grain growth and a significantly T-dependent variation of grain size distributions. The observations allow us to calibrate values for the grain size exponent n and the activation energy Q

  14. Molecular structure and metabolic characteristics of the proteins and energy in triticale grains and dried distillers grains with solubles for dairy cattle.

    Science.gov (United States)

    Liu, Bo; McKinnon, John J; Thacker, Philip; Yu, Peiqiang

    2012-10-10

    To our knowledge, there is no research on the molecular structure of triticale grain in comparison with other types of cereal grains and metabolic characteristics of the protein and energy in this grain and its coproducts, called dried distillers grains with solubles (DDGS), for dairy cattle. The objective of this study was to identify differences in molecular structures of proteins among grains and their DDGS using a molecular spectroscopy technique, namely, DRIFT, and to determine the nutrient profile and supply to dairy cattle. The protein molecular structure studies showed a difference (P triticale grain and DDGS. There were differences in the protein and carbohydrate subfractions (P triticale grain and DDGS. Triticale grain and DDGS had similar intestinal digestibility of rumen undegraded CP. However, triticale DDGS had higher (P triticale, indicating that triticale DDGS is a superior protein source for dairy cattle as compared with triticale grain. Bioethanol processing induced changes in the protein molecular structure.

  15. Carbohydrates of human immunodeficiency virus

    DEFF Research Database (Denmark)

    Hansen, J E

    1992-01-01

    Elucidation of the mechanism by which viral infection induces the appearance of carbohydrate neoantigens is highly important. Results from such studies could be expected to be significant for a general understanding of the regulation of glycosylation, and perhaps especially important for the unde...

  16. Racemic carbohydrates - fact or fiction?

    DEFF Research Database (Denmark)

    Senning, Alexander Erich Eugen

    2007-01-01

    Chemical Abstracts Service has developed unsound practices in the naming and handling of simple carbohydrates such as aldopentoses 1, aldohexoses 2, and ketohexoses 3. Typically, the common name glucose is sometimes, inappropriately, interpreted as meaning DL-glucose DL-2d. Thus, a considerable...

  17. Carbohydrate-based immune adjuvants

    Science.gov (United States)

    Petrovsky, Nikolai; Cooper, Peter D

    2011-01-01

    The role for adjuvants in human vaccines has been a matter of vigorous scientific debate, with the field hindered by the fact that for over 80 years, aluminum salts were the only adjuvants approved for human use. To this day, alum-based adjuvants, alone or combined with additional immune activators, remain the only adjuvants approved for use in the USA. This situation has not been helped by the fact that the mechanism of action of most adjuvants has been poorly understood. A relative lack of resources and funding for adjuvant development has only helped to maintain alum’s relative monopoly. To seriously challenge alum’s supremacy a new adjuvant has many major hurdles to overcome, not least being alum’s simplicity, tolerability, safety record and minimal cost. Carbohydrate structures play critical roles in immune system function and carbohydrates also have the virtue of a strong safety and tolerability record. A number of carbohydrate compounds from plant, bacterial, yeast and synthetic sources have emerged as promising vaccine adjuvant candidates. Carbohydrates are readily biodegradable and therefore unlikely to cause problems of long-term tissue deposits seen with alum adjuvants. Above all, the Holy Grail of human adjuvant development is to identify a compound that combines potent vaccine enhancement with maximum tolerability and safety. This has proved to be a tough challenge for many adjuvant contenders. Nevertheless, carbohydrate-based compounds have many favorable properties that could place them in a unique position to challenge alum’s monopoly over human vaccine usage. PMID:21506649

  18. Employing Lead Thiocyanate Additive to Reduce the Hysteresis and Boost the Fill Factor of Planar Perovskite Solar Cells

    Energy Technology Data Exchange (ETDEWEB)

    Ke, Weijun [Department of Physics and Astronomy and Wright Center for Photovoltaics Innovation and Commercialization, The University of Toledo, Toledo OH 43606 USA; Key Laboratory of Artificial Micro- and Nano-structures of Ministry of Education of China, School of Physics and Technology, Wuhan University, Wuhan 430072 China; National Renewable Energy Laboratory, 15013 Denver West Parkway Golden CO 80401 USA; Xiao, Chuanxiao [National Renewable Energy Laboratory, 15013 Denver West Parkway Golden CO 80401 USA; Wang, Changlei [Department of Physics and Astronomy and Wright Center for Photovoltaics Innovation and Commercialization, The University of Toledo, Toledo OH 43606 USA; Saparov, Bayrammurad [Department of Mechanical Engineering and Materials Science, Duke University, Durham NC 27708 USA; Department of Chemistry, Duke University, Durham NC 27708 USA; Duan, Hsin-Sheng [Department of Mechanical Engineering and Materials Science, Duke University, Durham NC 27708 USA; Zhao, Dewei [Department of Physics and Astronomy and Wright Center for Photovoltaics Innovation and Commercialization, The University of Toledo, Toledo OH 43606 USA; National Renewable Energy Laboratory, 15013 Denver West Parkway Golden CO 80401 USA; Xiao, Zewen [Department of Physics and Astronomy and Wright Center for Photovoltaics Innovation and Commercialization, The University of Toledo, Toledo OH 43606 USA; Schulz, Philip [National Renewable Energy Laboratory, 15013 Denver West Parkway Golden CO 80401 USA; Harvey, Steven P. [National Renewable Energy Laboratory, 15013 Denver West Parkway Golden CO 80401 USA; Liao, Weiqiang [Department of Physics and Astronomy and Wright Center for Photovoltaics Innovation and Commercialization, The University of Toledo, Toledo OH 43606 USA; Meng, Weiwei [Department of Physics and Astronomy and Wright Center for Photovoltaics Innovation and Commercialization, The University of Toledo, Toledo OH 43606 USA; Yu, Yue [Department of Physics and Astronomy and Wright Center for Photovoltaics Innovation and Commercialization, The University of Toledo, Toledo OH 43606 USA; Cimaroli, Alexander J. [Department of Physics and Astronomy and Wright Center for Photovoltaics Innovation and Commercialization, The University of Toledo, Toledo OH 43606 USA; Jiang, Chun-Sheng [National Renewable Energy Laboratory, 15013 Denver West Parkway Golden CO 80401 USA; Zhu, Kai [National Renewable Energy Laboratory, 15013 Denver West Parkway Golden CO 80401 USA; Al-Jassim, Mowafak [National Renewable Energy Laboratory, 15013 Denver West Parkway Golden CO 80401 USA; Fang, Guojia [Key Laboratory of Artificial Micro- and Nano-structures of Ministry of Education of China, School of Physics and Technology, Wuhan University, Wuhan 430072 China; Mitzi, David B. [Department of Mechanical Engineering and Materials Science, Duke University, Durham NC 27708 USA; Department of Chemistry, Duke University, Durham NC 27708 USA; Yan, Yanfa [Department of Physics and Astronomy and Wright Center for Photovoltaics Innovation and Commercialization, The University of Toledo, Toledo OH 43606 USA

    2016-05-04

    Lead thiocyanate in the perovskite precursor can increase the grain size of a perovskite thin film and reduce the conductivity of the grain boundaries, leading to perovskite solar cells with reduced hysteresis and enhanced fill factor. A planar perovskite solar cell with grain boundary and interface passivation achieves a steady-state efficiency of 18.42%.

  19. Employing Lead Thiocyanate Additive to Reduce the Hysteresis and Boost the Fill Factor of Planar Perovskite Solar Cells.

    Science.gov (United States)

    Ke, Weijun; Xiao, Chuanxiao; Wang, Changlei; Saparov, Bayrammurad; Duan, Hsin-Sheng; Zhao, Dewei; Xiao, Zewen; Schulz, Philip; Harvey, Steven P; Liao, Weiqiang; Meng, Weiwei; Yu, Yue; Cimaroli, Alexander J; Jiang, Chun-Sheng; Zhu, Kai; Al-Jassim, Mowafak; Fang, Guojia; Mitzi, David B; Yan, Yanfa

    2016-07-01

    Lead thiocyanate in the perovskite precursor can increase the grain size of a perovskite thin film and reduce the conductivity of the grain boundaries, leading to perovskite solar cells with reduced hysteresis and enhanced fill factor. A planar perovskite solar cell with grain boundary and interface passivation achieves a steady-state efficiency of 18.42%.

  20. Carbohydrate speciation and Py-MS mapping of peat samples from a subtropical open marsh environment

    Science.gov (United States)

    Moers, M. E. C.; Boon, J. J.; de Leeuw, J. W.; Baas, M.; Schenck, P. A.

    1989-08-01

    Neutral carbohydrates were determined as alditol acetates in the hydrolysates of fractionated peat samples from different depths from Rookery Branch (Florida Everglades), and of a Mariscus rhizome. The fine grained samples showed a major contribution of microbial sugars indicated by comparatively low total carbohydrate yields together with high relative contributions of galactose, mannose, ribose, fucose, rhamnose, partially methylated aldoses, amino sugars, heptoses, tetroses, glycerol, allose and altrose. The coarse grained samples showed a major contribution of vascular plant sugars indicated by comparatively high total carbohydrate yields together with high relative contributions of glucose, xylose and arabinose. Analysis of the fractionated peat samples by Curie point pyrolysis-mass spectrometry and multivariate analysis of the pyrolysis data yielded two discriminant functions. Depth profiles for the first discriminant function show a relative accumulation of lignified vascular plant material with increasing depth in coarse and fine grained samples. The finest grained samples show most of the markers indicative for microbial activity and the presence of refractory fine grained matter and least of the lignin markers. Plots of the second discriminant function versus depth reveal an initial increase in markers for modified lignin, indicating degradation. The deepest sample, however, again shows increased characteristics for intact vascular plant polysaccharides. The results from both wet chemical analysis and Py-MS suggest the presence of living and/or senescent rootlets at the deepest level in the peat.

  1. Interactions of carbohydrates and proteins by fluorophore-assisted carbohydrate electrophoresis

    Indian Academy of Sciences (India)

    Gang-Liang Huang; Xin-Ya Mei; Peng-George Wang

    2006-06-01

    A sensitive, specific, and rapid method for the detection of carbohydrate-protein interactions is demonstrated by fluorophore-assisted carbohydrate electrophoresis (FACE). The procedure is simple and the cost is low. The advantage of this method is that carbohydrate-protein interactions can be easily displayed by FACE, and the carbohydrates do not need to be purified.

  2. Abrasion properties of homogenous and blended fill materials during pressure hydraulic transport

    Energy Technology Data Exchange (ETDEWEB)

    Turchaninov, S.P.

    1978-03-01

    A description is given of tests conducted to determine the abrasive properties of small and large-grain free-flowing fill materials during hydraulic transport of the materials under pressure. Data are given on the size, density, abrasiveness of various sized varieties of rock, sand, and blends comprising homogenous materials, simple and complex mixtures, and on the physical characteristics of various fill materials in relation to the trafficability and parameters of pipelines. Technical specifications are given for fill steel pipes. The study indicates that the durability of hydraulic fill pipelines largely depends on the abrasiveness of the fill materials. 3 references, 2 figures, 2 tables.

  3. Hydrogen Filling Station

    Energy Technology Data Exchange (ETDEWEB)

    Boehm, Robert F; Sabacky, Bruce; Anderson II, Everett B; Haberman, David; Al-Hassin, Mowafak; He, Xiaoming; Morriseau, Brian

    2010-02-24

    future. Project partners also conducted a workshop on hydrogen safety and permitting. This provided an opportunity for the various permitting agencies and end users to gather to share experiences and knowledge. As a result of this workshop, the permitting process for the hydrogen filling station on the Las Vegas Valley Water District’s land was done more efficiently and those who would be responsible for the operation were better educated on the safety and reliability of hydrogen production and storage. The lessons learned in permitting the filling station and conducting this workshop provided a basis for future hydrogen projects in the region. Continuing efforts to increase the working pressure of electrolysis and efficiency have been pursued. Research was also performed on improving the cost, efficiency and durability of Proton Exchange Membrane (PEM) hydrogen technology. Research elements focused upon PEM membranes, electrodes/catalysts, membrane-electrode assemblies, seals, bipolar plates, utilization of renewable power, reliability issues, scale, and advanced conversion topics. Additionally, direct solar-to-hydrogen conversion research to demonstrate stable and efficient photoelectrochemistry (PEC) hydrogen production systems based on a number of optional concepts was performed. Candidate PEC concepts included technical obstacles such as inefficient photocatalysis, inadequate photocurrent due to non-optimal material band gap energies, rapid electron-hole recombination, reduced hole mobility and diminished operational lifetimes of surface materials exposed to electrolytes. Project Objective 1: Design, build, operate hydrogen filling station Project Objective 2: Perform research and development for utilizing solar technologies on the hydrogen filling station and convert two utility vehicles for use by the station operators Project Objective 3: Increase capacity of hydrogen filling station; add additional vehicle; conduct safety workshop; develop a roadmap for

  4. Hydrogen Filling Station

    Energy Technology Data Exchange (ETDEWEB)

    Boehm, Robert F; Sabacky, Bruce; Anderson II, Everett B; Haberman, David; Al-Hassin, Mowafak; He, Xiaoming; Morriseau, Brian

    2010-02-24

    future. Project partners also conducted a workshop on hydrogen safety and permitting. This provided an opportunity for the various permitting agencies and end users to gather to share experiences and knowledge. As a result of this workshop, the permitting process for the hydrogen filling station on the Las Vegas Valley Water District’s land was done more efficiently and those who would be responsible for the operation were better educated on the safety and reliability of hydrogen production and storage. The lessons learned in permitting the filling station and conducting this workshop provided a basis for future hydrogen projects in the region. Continuing efforts to increase the working pressure of electrolysis and efficiency have been pursued. Research was also performed on improving the cost, efficiency and durability of Proton Exchange Membrane (PEM) hydrogen technology. Research elements focused upon PEM membranes, electrodes/catalysts, membrane-electrode assemblies, seals, bipolar plates, utilization of renewable power, reliability issues, scale, and advanced conversion topics. Additionally, direct solar-to-hydrogen conversion research to demonstrate stable and efficient photoelectrochemistry (PEC) hydrogen production systems based on a number of optional concepts was performed. Candidate PEC concepts included technical obstacles such as inefficient photocatalysis, inadequate photocurrent due to non-optimal material band gap energies, rapid electron-hole recombination, reduced hole mobility and diminished operational lifetimes of surface materials exposed to electrolytes. Project Objective 1: Design, build, operate hydrogen filling station Project Objective 2: Perform research and development for utilizing solar technologies on the hydrogen filling station and convert two utility vehicles for use by the station operators Project Objective 3: Increase capacity of hydrogen filling station; add additional vehicle; conduct safety workshop; develop a roadmap for

  5. Seed filling in domesticated maize and rice depends on SWEET-mediated hexose transport

    Science.gov (United States)

    Carbohydrate import into seeds directly determines seed size and must have been increased through domestication. However, evidence for domestication of sugar translocation and the identity of seed filling transporters remained elusive. Maize ZmSWEET4c, as opposed to its sucrose-transporting homologs...

  6. Grain Unloading of Arsenic Species in Rice

    Energy Technology Data Exchange (ETDEWEB)

    Carey, Anne-Marie; Scheckel, Kirk G.; Lombi, Enzo; Newville, Matt; Choi, Yongseong; Norton, Gareth J.; Charnock, John M.; Feldmann, Joerg; Price, Adam H.; Meharg, Andrew A. (EPA); (U. South Australia); (Manchester); (Aberdeen); (UC)

    2010-01-11

    Rice (Oryza sativa) is the staple food for over half the world's population yet may represent a significant dietary source of inorganic arsenic (As), a nonthreshold, class 1 human carcinogen. Rice grain As is dominated by the inorganic species, and the organic species dimethylarsinic acid (DMA). To investigate how As species are unloaded into grain rice, panicles were excised during grain filling and hydroponically pulsed with arsenite, arsenate, glutathione-complexed As, or DMA. Total As concentrations in flag leaf, grain, and husk, were quantified by inductively coupled plasma mass spectroscopy and As speciation in the fresh grain was determined by x-ray absorption near-edge spectroscopy. The roles of phloem and xylem transport were investigated by applying a {+-} stem-girdling treatment to a second set of panicles, limiting phloem transport to the grain in panicles pulsed with arsenite or DMA. The results demonstrate that DMA is translocated to the rice grain with over an order magnitude greater efficiency than inorganic species and is more mobile than arsenite in both the phloem and the xylem. Phloem transport accounted for 90% of arsenite, and 55% of DMA, transport to the grain. Synchrotron x-ray fluorescence mapping and fluorescence microtomography revealed marked differences in the pattern of As unloading into the grain between DMA and arsenite-challenged grain. Arsenite was retained in the ovular vascular trace and DMA dispersed throughout the external grain parts and into the endosperm. This study also demonstrates that DMA speciation is altered in planta, potentially through complexation with thiols.

  7. Disorders of carbohydrate digestion and absorption.

    Science.gov (United States)

    Heitlinger, L A; Lebenthal, E

    1988-04-01

    The carbohydrate malabsorptive syndromes are frequently seen by pediatricians. The congenital deficiency states are quite rare, but adult type hypolactasia and lactose intolerance following rotavirus infection are recognized with increasing frequency by primary care physicians. Therapy for these disorders involves identification of the offending carbohydrate, removal of the carbohydrate from the diet, and exclusion of other entities that may result in carbohydrate malabsorption but not respond to its removal from the diet. Prognosis for both the primary and secondary carbohydrate malabsorption syndromes is excellent. Compliance with diets for those pediatric patients who will require lifelong therapy remains problematic.

  8. Preparing for faster filling

    CERN Multimedia

    CERN Bulletin

    2010-01-01

    Following the programmed technical stop last week, operators focussed on preparing the machine for faster filling, which includes multibunch injection and a faster pre-cycle phase.   The LHC1 screen shot during the first multibunch injection operation. The LHC operational schedule incorporates a technical stop for preventive maintenance roughly every six weeks of stable operation, during which several interventions on the various machines are carried out. Last week these included the replacement of a faulty magnet in the SPS pre-accelerator, which required the subsequent re-setting of the system of particle extraction and transfer to the LHC. At the end of last week, all the machines were handed back for operation and work could start on accommodating all the changes made into the complex systems in order for normal operation to be resumed. These ‘recovery’ operations continued through the weekend and into this week. At the beginning of this week, operators succeeded in pro...

  9. Bulk-Fill Resin Composites

    DEFF Research Database (Denmark)

    Benetti, Ana Raquel; Havndrup-Pedersen, Cæcilie; Honoré, Daniel;

    2015-01-01

    the restorative procedure. The aim of this study, therefore, was to compare the depth of cure, polymerization contraction, and gap formation in bulk-fill resin composites with those of a conventional resin composite. To achieve this, the depth of cure was assessed in accordance with the International Organization...... for Standardization 4049 standard, and the polymerization contraction was determined using the bonded-disc method. The gap formation was measured at the dentin margin of Class II cavities. Five bulk-fill resin composites were investigated: two high-viscosity (Tetric EvoCeram Bulk Fill, SonicFill) and three low......-viscosity (x-tra base, Venus Bulk Fill, SDR) materials. Compared with the conventional resin composite, the high-viscosity bulk-fill materials exhibited only a small increase (but significant for Tetric EvoCeram Bulk Fill) in depth of cure and polymerization contraction, whereas the low-viscosity bulk...

  10. Carbohydrate Metabolism in Submariner Personnel

    Science.gov (United States)

    1983-06-01

    metabolism the Wilkerson Point System, for glucose values, used in conjunction with patterns of insulin response described by Kraft(4) serves as the means...amount of exercise and carbohydrate metabolism characteristics occurred in both submariners and non-submariners. An inverse relationship also seems to...individuals(7). In the present study a significant negative correlation was also found between exercise vs one and two hour postprandial glucose and two hour

  11. Carbohydrates Through Animation: Preliminary Step

    OpenAIRE

    J.K. Sugai; M.S.R. Figueiredo; ANTÔNIO, R. V.; P. M. Oliveira; V.A Cardoso; Ricardo, J.; Merino, E.; L.F Figueiredo; D.N. Heidrich

    2004-01-01

    Methods of education are changing, so the educational tools must change too. The developmentof the systems of information and communication gave the opportunity to bring new technology tothe learning process. Modern education needs interactive programs that may be available to theacademic community, in order to ease the learning process and sharing of the knowledge. Then,an educational software on Carbohydrates is being developed using concept maps and FLASH-MXanimations program, and approach...

  12. Grain Accumulation of Selenium Species in Rice (Oryza sativa L.)

    Energy Technology Data Exchange (ETDEWEB)

    Carey, Anne-Marie; Scheckel, Kirk G.; Lombi, Enzo; Newville, Matt; Choi, Yongseong; Norton, Gareth J.; Price, Adam H.; Meharg, Andrew A. (EPA); (U. South Australia); (Aberdeen); (UC)

    2012-09-05

    Efficient Se biofortification programs require a thorough understanding of the accumulation and distribution of Se species within the rice grain. Therefore, the translocation of Se species to the filling grain and their spatial unloading were investigated. Se species were supplied via cut flag leaves of intact plants and excised panicle stems subjected to a {+-} stem-girdling treatment during grain fill. Total Se concentrations in the flag leaves and grain were quantified by inductively coupled plasma mass spectrometry. Spatial accumulation was investigated using synchrotron X-ray fluorescence microtomography. Selenomethionine (SeMet) and selenomethylcysteine (SeMeSeCys) were transported to the grain more efficiently than selenite and selenate. SeMet and SeMeSeCys were translocated exclusively via the phloem, while inorganic Se was transported via both the phloem and xylem. For SeMet- and SeMeSeCys-fed grain, Se dispersed throughout the external grain layers and into the endosperm and, for SeMeSeCys, into the embryo. Selenite was retained at the point of grain entry. These results demonstrate that the organic Se species SeMet and SeMeSeCys are rapidly loaded into the phloem and transported to the grain far more efficiently than inorganic species. Organic Se species are distributed more readily, and extensively, throughout the grain than selenite.

  13. Effects of Post-Anthesis Drought and Waterlogging on Accumulation of High-Molecular-Weight Glutenin Subunits and Glutenin Macropolymers Content in Wheat Grain

    DEFF Research Database (Denmark)

    Jiang, D; Yue, H; Wollenweber, B;

    2009-01-01

    Drought and flooding during grain filling have become major constraints to wheat quality and yield. The impacts of water deficits and waterlogging during the grain filling on contents of high-molecular-weight glutenin subunits (HMW-GS) and of glutenin macropolymers (GMP) in grains of the winter w...

  14. Cereal Grains: Their Impacts on Health and Food Safety

    Institute of Scientific and Technical Information of China (English)

    P Rayas-Duarte; J Uriyapongson

    2006-01-01

    Cereal grains can contribute to maintain health and prevent chronic diseases by supplying biologically active components. The exact mechanism of action of these components is not completely understood, but extensive evidence suggests the antioxidants that are present in the grains are the likely source of benefits. These activities or properties might protect against coronary heart disease and diabetes. The relationships of complex carbohydrates, whole grain products and the prevention of diseases, including type2 diabetes mellitus and coronary heart disease continue to challenge the scientific community. Though cereal grains contribute to a healthy diet there are some people that can not tolerate the proteins present in the grain. This can lead to food allergies and when severe can be diagnosed as celiac disease.

  15. Derivation of Hillert-type 3D grain growth rate model with topological considerations and discussion on its grain size parameter

    Institute of Scientific and Technical Information of China (English)

    Guoquan Liu; Haibo Yu; Xiaoyan Song; Xiangge Qin; Chao Wang

    2004-01-01

    A Hillert-type three-dimensional grain growth rate model was derived through the grain topology-size correlation model,combined with a topology-dependent grain growth rate equation in three dimensions. It shows clearly that the Hillert-type 3D grain growth rate model may also be described with topology considerations of microstructure. The size parameter bearing in the model is further discussed both according to the derived model and in another approach with the aid of quantitative relationship between the grain size and the integral mean curvature over grain surface. Both approaches successfully demonstrate that, if the concerned grains can be well approximated by a space-filling convex polyhedra in shape, the grain size parameter bearing in the Hillert-type 3D grain growth model should be a parameter proportional to the mean grain tangent radius.

  16. A systematic study of chemogenomics of carbohydrates.

    Science.gov (United States)

    Gu, Jiangyong; Luo, Fang; Chen, Lirong; Yuan, Gu; Xu, Xiaojie

    2014-03-04

    Chemogenomics focuses on the interactions between biologically active molecules and protein targets for drug discovery. Carbohydrates are the most abundant compounds in natural products. Compared with other drugs, the carbohydrate drugs show weaker side effects. Searching for multi-target carbohydrate drugs can be regarded as a solution to improve therapeutic efficacy and safety. In this work, we collected 60 344 carbohydrates from the Universal Natural Products Database (UNPD) and explored the chemical space of carbohydrates by principal component analysis. We found that there is a large quantity of potential lead compounds among carbohydrates. Then we explored the potential of carbohydrates in drug discovery by using a network-based multi-target computational approach. All carbohydrates were docked to 2389 target proteins. The most potential carbohydrates for drug discovery and their indications were predicted based on a docking score-weighted prediction model. We also explored the interactions between carbohydrates and target proteins to find the pathological networks, potential drug candidates and new indications.

  17. Reproductive performance of reindeer fed all-grain and hay-grain rations

    Directory of Open Access Journals (Sweden)

    J. M. Blanchard

    1986-06-01

    Full Text Available Reproductive performance of grain-fed reindeer {Rangifer tarandus was evaluated over a 2-year period. Groups of pregnant reindeer were fed one of three rations, (1 100% whole-grain barley, (2 98.9% whole-grain barley and 1.2% mineral and trace element supplement, and (3 70% whole-grain barley and 30% finely-chopped bluegrass hay. Reindeer fed unsupplemented whole-barley failed to produce a single live calf. The addition of mineral and trace element supplement to the ration did not result in any significant improvement in reproductive performance. Eighty-five percent of the reindeer consuming unsupplemented and supplemented all-barley rations became pregnant; however, 76% of the pregnancies resulted in stillborn calves. One-hundred percent of the cows maintained on the grain/hay ration produced live calves. We speculate that reproductive failure in reindeer cows maintained on all-grain rations is most likely a result of a diet induced disfunction in maternal rumen and/or carbohydrate metabolism rather than a micro-nutrient deficiency. More research is neeeded to determine which metabolic pathways are affected.

  18. Surface characterization of carbohydrate microarrays.

    Science.gov (United States)

    Scurr, David J; Horlacher, Tim; Oberli, Matthias A; Werz, Daniel B; Kroeck, Lenz; Bufali, Simone; Seeberger, Peter H; Shard, Alexander G; Alexander, Morgan R

    2010-11-16

    Carbohydrate microarrays are essential tools to determine the biological function of glycans. Here, we analyze a glycan array by time-of-flight secondary ion mass spectrometry (ToF-SIMS) to gain a better understanding of the physicochemical properties of the individual spots and to improve carbohydrate microarray quality. The carbohydrate microarray is prepared by piezo printing of thiol-terminated sugars onto a maleimide functionalized glass slide. The hyperspectral ToF-SIMS imaging data are analyzed by multivariate curve resolution (MCR) to discern secondary ions from regions of the array containing saccharide, linker, salts from the printing buffer, and the background linker chemistry. Analysis of secondary ions from the linker common to all of the sugar molecules employed reveals a relatively uniform distribution of the sugars within the spots formed from solutions with saccharide concentration of 0.4 mM and less, whereas a doughnut shape is often formed at higher-concentration solutions. A detailed analysis of individual spots reveals that in the larger spots the phosphate buffered saline (PBS) salts are heterogeneously distributed, apparently resulting in saccharide concentrated at the rim of the spots. A model of spot formation from the evaporating sessile drop is proposed to explain these observations. Saccharide spot diameters increase with saccharide concentration due to a reduction in surface tension of the saccharide solution compared to PBS. The multivariate analytical partial least squares (PLS) technique identifies ions from the sugars that in the complex ToF-SIMS spectra correlate with the binding of galectin proteins.

  19. Applications of synthetic carbohydrates to chemical biology.

    Science.gov (United States)

    Lepenies, Bernd; Yin, Jian; Seeberger, Peter H

    2010-06-01

    Access to synthetic carbohydrates is an urgent need for the development of carbohydrate-based drugs, vaccines, adjuvants as well as novel drug delivery systems. Besides traditional synthesis in solution, synthetic carbohydrates have been generated by chemoenzymatic methods as well as automated solid-phase synthesis. Synthetic oligosaccharides have proven to be useful for identifying ligands of carbohydrate-binding proteins such as C-type lectins and siglecs using glycan arrays. Furthermore, glyconanoparticles and glycodendrimers have been used for specific targeting of lectins of the immune system such as selectins, DC-SIGN, and CD22. This review focuses on how diverse carbohydrate structures can be synthetically derived and highlights the benefit of synthetic carbohydrates for glycobiology.

  20. New fabrication and applications of carbohydrate arrays.

    Science.gov (United States)

    Huang, Gangliang; Chen, Xin; Xiao, Feng

    2014-01-01

    Carbohydrate arrays are used as high-throughput screening platforms to study the carbohydrate-mediated recognition events for glycobiology. The polysaccharide arrays are easy to fabricate by non-covalently or covalently immobilizing polysaccharides onto array surfaces because polysaccharides have hydrophobic interactions. Oligosaccharides must be derived and covalently or non-covalently immobilized onto array surfaces to fabricate oligosaccharide arrays because they have hydrophilic interactions. At the moment, carbohydrate arrays are mainly used to study the carbohydrate-protein interactions and carbohydrate-binding lectins or antibodies, which are possible to be applied to clinics and diagnoses. This review mainly summed up the new fabrication strategies of carbohydrate arrays and their applications in recent four years.

  1. Microbiota of kefir grains

    Directory of Open Access Journals (Sweden)

    Tomislav Pogačić

    2013-03-01

    Full Text Available Kefir grains represent the unique microbial community consisting of bacteria, yeasts, and sometimes filamentous moulds creating complex symbiotic community. The complexity of their physical and microbial structures is the reason that the kefir grains are still not unequivocally elucidated. Microbiota of kefir grains has been studied by many microbiological and molecular approaches. The development of metagenomics, based on the identification without cultivation, is opening new possibilities for identification of previously nonisolated and non-identified microbial species from the kefir grains. Considering recent studies, there are over 50 microbial species associated with kefir grains. The aim of this review is to summarise the microbiota composition of kefir grains. Moreover, because of technological and microbiological significance of the kefir grains, the paper provides an insight into the microbiological and molecular methods applied to study microbial biodiversity of kefir grains.

  2. Low-carbohydrate nutrition and metabolism.

    Science.gov (United States)

    Westman, Eric C; Feinman, Richard D; Mavropoulos, John C; Vernon, Mary C; Volek, Jeff S; Wortman, James A; Yancy, William S; Phinney, Stephen D

    2007-08-01

    The persistence of an epidemic of obesity and type 2 diabetes suggests that new nutritional strategies are needed if the epidemic is to be overcome. A promising nutritional approach suggested by this thematic review is carbohydrate restriction. Recent studies show that, under conditions of carbohydrate restriction, fuel sources shift from glucose and fatty acids to fatty acids and ketones, and that ad libitum-fed carbohydrate-restricted diets lead to appetite reduction, weight loss, and improvement in surrogate markers of cardiovascular disease.

  3. Digestion of carbohydrates in the pig.

    Science.gov (United States)

    Drochner, W

    1993-01-01

    A review of carbohydrate digestion in the pig is given. The cascade of digestion in the mouth, stomach, small and large intestine is described. Principles of enzymatic and fermentative digestion according to new results with fistulated animals are discussed. The efficacy and quality of fermentation in the large intestine depending on level and quality of carbohydrates in the diet are demonstrated. Some aspects of energetical efficacy of hindgut digestion are discussed. Dietetic effects of carbohydrates are described.

  4. Regioselective azidotrimethylsilylation of carbohydrates and applications thereof.

    Science.gov (United States)

    L, Mallikharjuna Rao; Yousuf, Syed Khalid; Mukherjee, Debaraj; Taneja, Subhash Chandra

    2012-12-07

    Azidotrimethylsilylation of carbohydrates (monosaccharides and disaccharides) has been achieved in high yields under Mitsunobu conditions. The azidation of carbohydrates is effected at 0 °C essentially only at the primary alcoholic position in mono, di- and triols in protected/unprotected glycosides, whereas the remaining secondary hydroxyl groups got silylated. Surprisingly, no azidation of the secondary hydroxyls was observed in all the carbohydrate substrates. Applications of the methodology for the synthesis of amino sugars, triazoles and azasugars are reported.

  5. The effects of carbohydrate variation in isocaloric diets on glycogenolysis and gluconeogenesis in healthy men

    NARCIS (Netherlands)

    Bisschop, PH; Arias, AMP; Ackermans, MT; Endert, E; Pijl, H; Kuipers, F; Meijer, AJ; Sauerwein, HP; Romijn, JA

    2000-01-01

    To evaluate the effect of dietary carbohydrate content on postabsorptive glucose metabolism, we quantified gluconeogenesis and glycogenolysis after 11 days of high carbohydrate (85% carbohydrate), control (44% carbohydrate), and very low carbohydrate (2% carbohydrate) diets in six healthy men. Diets

  6. Mechanics of filled carbon nanotubes

    KAUST Repository

    Monteiro, A.O.

    2014-04-01

    The benefits of filling carbon nanotubes (CNTs) with assorted molecular and crystalline substances have been investigated for the past two decades. Amongst the study of new structural phases, defects, chemical reactions and varied types of host-guest interactions, there is one fundamental characterisation aspect of these systems that continues to be overlooked: the mechanical behaviour of filled CNTs. In contrast to their empty counterparts, the mechanics of filled CNTs is a subject where reports appear far and apart, this despite being key to the application of these materials in technological devices. In the following paragraphs, we review the work that has been carried out up to the present on the mechanics of filled CNTs. The studies discussed range from experimental resonant frequency essays performed within electron microscopes to modelling, via molecular dynamics, of three-point bending of nanotubes filled with gases. (C) 2014 Elsevier B.V. All rights reserved.

  7. Regioselective monodeprotection of peracetylated carbohydrates.

    Science.gov (United States)

    Filice, Marco; Guisan, Jose M; Terreni, Marco; Palomo, Jose M

    2012-10-01

    This protocol describes the regioselective deprotection of single hydroxyls in peracetylated monosaccharides and disaccharides by enzymatic or chemoenzymatic strategies. The introduction of a one-pot enzymatic step by using immobilized biocatalysts obviates the requirement to carry out tedious workups and time-consuming purifications. By using this straightforward protocol, different per-O-acetylated glycopyranosides (mono- or disaccharides, 1-substituted or glycals) can be transformed into a whole set of differentially monodeprotected 1-alcohols, 3-alcohols, 4-alcohols and 6-alcohols in high yields. These tailor-made glycosyl acceptors can then be used for stereoselective glycosylation for oligosaccharide and glycoderivative synthesis. They have been successfully used as building blocks to synthesize tailor-made di- and trisaccharides involved in the structure of lacto-N-neo-tetraose and precursors of the tumor-associated carbohydrate antigen T and the antitumoral drug peracetylated β-naphtyl-lactosamine. We are able to prepare a purified monoprotected carbohydrate in between 1 and 4 d. With this protocol, the small library of monodeprotected products can be synthesized in 1-2 weeks.

  8. Effects of MeJA on the Physiological Characteristics of japonica Rice Wuyun-jing 24 and Ningjing 3 During Early Grain Filling Stage Under Heat Stress%茉莉酸甲酯对武运粳24和宁粳3号灌浆早期高温胁迫生理特性的影响

    Institute of Scientific and Technical Information of China (English)

    刘霞; 唐设; 窦志; 李刚华; 刘正辉; 王绍华; 丁承强; 丁艳锋

    2016-01-01

    Methyl jasmonate (MeJA) was used on the conventional japonica rice varieties Wuyunjing 24 and Ningjing 3 to study its effects on rice physiological characteristics under high temperature stress during early grain filling stage . The results indicated that MeJA could increase the accumulation of soluble sugar in rice leaf and keep the osmotic balances .Furthermore ,MeJA increased the stomatal conductance (Gs ) and transpiration rate (Tr ) ,which accordingly reduced the temperature of leaf and panicle and further effectively alleviated the high temperature damages .Exogenous MeJA reduced the heat damages to the photosynthetic system , thereby leading to the increased PS Ⅱ maximum quantum yield (Fv/Fm ) ,photochemical efficiency (ΦPSⅡ ) ,photosynthetic electron transport rate (ETR) ,energy ratio ofphotochemicalreactions(Pr)andnetphotosyntheticrate(Pn).Increasedgrainweightandseedsettingratewere found in those MeJA treated rice plants and antioxidant enzyme activity analysis showed that when exposed to high temperature for 12 days , superoxide dismutase (SOD ) and peroxidase (POD ) antioxidant enzyme activities of Wuyunjing 24 were decreased ,while SOD and POD activities of Ningjing 3 in late stage of high‐temperature treatment were greatly increased compared to the CK .The results indicated that MeJA enhanced the activities of SOD and POD and reduced the accumulation of malonaldehyde(MDA) ,which ultimately increased the membrane stability .In conclu‐sion ,exogenous MeJA could reduce the rice canopy temperature and effectively relieve heat stress injury to plant physi‐ological characteristics .Furthermore ,exogenous MeJA could promote recovery function during recovery stage and fi‐nally mitigate yield losses .%以武运粳24和宁粳3号两个常规粳稻品种为材料,采用桶栽方式,研究高温条件下外源茉莉酸甲酯(M eJA )在灌浆早期对水稻生理特性及产量的影响。结果显示,喷施外源M eJA可以提高植株体

  9. Calculating Cross Sections of Composite Interstellar Grains

    CERN Document Server

    Voshchinnikov, N V; Voshchinnikov, Nikolai V.; Mathis, John S.

    1999-01-01

    Interstellar grains may be composite collections of particles of distinct materials, including voids, agglomerated together. We determine the various optical cross sections of such composite grains, given the optical properties of each constituent, using an approximate model of the composite grain. We assume it consists of many concentric spherical layers of the various materials, each with a specified volume fraction. In such a case the usual Mie theory can be generalized and the extinction, scattering, and other cross sections determined exactly. We find that the ordering of the materials in the layering makes some difference to the derived cross sections, but averaging over the various permutations of the order of the materials provides rapid convergence as the number of shells (each of which is filled by all of the materials proportionately to their volume fractions) is increased. Three shells, each with one layer of a particular constituent material, give a very satisfactory estimate of the average cross...

  10. Biochemical software: Carbohydrates on Laboratory

    Directory of Open Access Journals (Sweden)

    D.N. Heidrich

    2005-07-01

    Full Text Available Educators around  the  world  are  being  challenged  to  develop  and  design  better and  more  effective strategies for student learning  using a variety  of modern  resources.  In this  present  work, an educa- tional  hypermedia  software  was constructed as a support tool to biochemistry teaching.  Occurrence, structure, main  characteristics and  biological  function  of the  biomolecule  Carbohydrates were pre- sented  through  modules.  The  software was developed  using concept  maps,  ISIS-Draw,  and  FLASH- MX animation program.  The chapter  Carbohydrates on Laboratory illustrates experimental methods of carbohydrates characterization, through  animation of a laboratory scenery.   The  subject was de- veloped showing reactions  as Bial, Benedict, Selliwanoff, Barfoed, Phenol  Sulphuric,  and Iodines, and also enzymatic  reactions  as glucose oxidase and amylase.  There are also links with short texts  in order to help the understanding of the contents  and principles of laboratory practice  as well as background reactions. Application of the software to undergraduate students and high school teachers  showed an excellent  acceptance.   All of them  considered  the  software  a very good learning  tool.  Both  teachers and students welcomed this program  as it is more flexible, and allows the learning in a more individual rhythm. In addition, application of the software would be suitable  to a more effective learning  and it is less expensive than conventional experimental teaching.

  11. THE INSTABILITY OF THE DIFFUSION-CONTROLLED GRAIN-BOUNDARY VOID IN STRESSED SOLID

    Institute of Scientific and Technical Information of China (English)

    王华; 李中华

    2003-01-01

    As atoms migrate along a void surface and grain-boundary, driven by various thermodynamic forces, the grain-boundary void changes its shape and volume. When the void changes its configuration, the free energy of the system also changes. In this article, the free energy is calculated for an evolving grain-boundary void filled with gas in a stressed solid. Then the instability conditions and the equilibrium shape of the void are determined as a function of the grain-boundary and surface energies, the void volume, the externally applied stresses, as well as the internal pressure built up by the gas filled in the void.

  12. Wood adhesives containing proteins and carbohydrates

    Science.gov (United States)

    In recent years there has been resurgent interest in using biopolymers as sustainable and environmentally friendly ingredients in wood adhesive formulations. Among them, proteins and carbohydrates are the most commonly used. In this chapter, an overview is given of protein-based and carbohydrate-...

  13. Mastering ectomycorrhizal symbiosis: the impact of carbohydrates.

    Science.gov (United States)

    Nehls, Uwe

    2008-01-01

    Mycorrhiza formation is the consequence of a mutualistic interaction between certain soil fungi and plant roots that helps to overcome nutritional limitations faced by the respective partners. In symbiosis, fungi contribute to tree nutrition by means of mineral weathering and mobilization of nutrients from organic matter, and obtain plant-derived carbohydrates as a response. Support with easily degradable carbohydrates seems to be the driving force for fungi to undergo this type of interaction. As a consequence, the fungal hexose uptake capacity is strongly increased in Hartig net hyphae of the model fungi Amanita muscaria and Laccaria bicolor. Next to fast carbohydrate uptake and metabolism, storage carbohydrates are of special interest. In functional A. muscaria ectomycorrhizas, expression and activity of proteins involved in trehalose biosynthesis is mainly localized in hyphae of the Hartig net, indicating an important function of trehalose in generation of a strong carbon sink by fungal hyphae. In symbiosis, fungal partners receive up to approximately 19 times more carbohydrates from their hosts than normal leakage of the root system would cause, resulting in a strong carbohydrate demand of infected roots and, as a consequence, a more efficient plant photosynthesis. To avoid fungal parasitism, the plant seems to have developed mechanisms to control carbohydrate drain towards the fungal partner and link it to the fungus-derived mineral nutrition. In this contribution, current knowledge on fungal strategies to obtain carbohydrates from its host and plant strategies to enable, but also to control and restrict (under certain conditions), carbon transfer are summarized.

  14. Carbohydrate clearance receptors in transfusion medicine

    DEFF Research Database (Denmark)

    Sørensen, Anne Louise Tølbøll; Clausen, Henrik; Wandall, Hans H

    2012-01-01

    Complex carbohydrates play important functions for circulation of proteins and cells. They provide protective shields and refraction from non-specific interactions with negative charges from sialic acids to enhance circulatory half-life. For recombinant protein therapeutics carbohydrates are espe...

  15. Vacuum ultraviolet photoionization of carbohydrates and nucleotides

    Energy Technology Data Exchange (ETDEWEB)

    Shin, Joong-Won, E-mail: jshin@govst.edu [Division of Science, Governors State University, University Park, Illinois 60484-0975 (United States); Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523-1872 (United States); Bernstein, Elliot R., E-mail: erb@lamar.colostate.edu [Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523-1872 (United States)

    2014-01-28

    Carbohydrates (2-deoxyribose, ribose, and xylose) and nucleotides (adenosine-, cytidine-, guanosine-, and uridine-5{sup ′}-monophosphate) are generated in the gas phase, and ionized with vacuum ultraviolet photons (VUV, 118.2 nm). The observed time of flight mass spectra of the carbohydrate fragmentation are similar to those observed [J.-W. Shin, F. Dong, M. Grisham, J. J. Rocca, and E. R. Bernstein, Chem. Phys. Lett. 506, 161 (2011)] for 46.9 nm photon ionization, but with more intensity in higher mass fragment ions. The tendency of carbohydrate ions to fragment extensively following ionization seemingly suggests that nucleic acids might undergo radiation damage as a result of carbohydrate, rather than nucleobase fragmentation. VUV photoionization of nucleotides (monophosphate-carbohydrate-nucleobase), however, shows that the carbohydrate-nucleobase bond is the primary fragmentation site for these species. Density functional theory (DFT) calculations indicate that the removed carbohydrate electrons by the 118.2 nm photons are associated with endocyclic C–C and C–O ring centered orbitals: loss of electron density in the ring bonds of the nascent ion can thus account for the observed fragmentation patterns following carbohydrate ionization. DFT calculations also indicate that electrons removed from nucleotides under these same conditions are associated with orbitals involved with the nucleobase-saccharide linkage electron density. The calculations give a general mechanism and explanation of the experimental results.

  16. Derivatization Reaction of Carbohydrates with Urea as the Reagent and Fluorimetric Determination of Carbohydrates

    Institute of Scientific and Technical Information of China (English)

    YANG,Jing-He(杨景和); CAO,Xi-Hui(曹西慧); WANG,Min(王敏); WU,Xia(吴霞); SUN,Chang-Xia(孙长侠)

    2002-01-01

    It is found that in the presence of sulfuric acid carbohydrates condense with urea to afford the condensation products, which emit fluorescence. Under optimum conditions, the fluorescence intensities of system are proportional to the concentrations of carbohydrates. Based on this linear relationship,quantitative determination of kinds of carbohydrates has been made. Among an the carbohydrates tested, the sensitivity of α-rhamnose is the highest and its limits of detection reaches 3.5 × 10-8 mol/L. So α-rhamnose can be selectively determed in the presence of other carbohydrates. A interaction mechanism is also discussed.

  17. Industrial applications of marine carbohydrates.

    Science.gov (United States)

    Sudha, Prasad N; Aisverya, S; Nithya, R; Vijayalakshmi, K

    2014-01-01

    Biomaterials have been used increasingly in various fields, such as drug delivery, imaging, and tissue engineering. The main reason justifying the widespread use of biomaterials relies on its valuable and low-cost source of new drugs. Current research goals are focused on identifying more potent and specific compounds with antitumor, immunomodulatory, antihyperlipidemic, anticoagulant, and antiviral activities. The increasing knowledge of structural analysis and chemical modifications enables the use of these marine carbohydrates in a newer way for the human welfare. This chapter focuses on the recent developments related to industrial and biomedical applications using chitin, chitosan, alginate, agar, and carrageenan derivatives and reports the main advances published over the last 10-15 years.

  18. Nondigestible carbohydrates and mineral bioavailability.

    Science.gov (United States)

    Greger, J L

    1999-07-01

    Generally, fiber and compounds associated with fiber in cereal products (e.g., phytates) have been found to reduce the apparent absorption of minerals (such as calcium, magnesium, zinc and manganese) in humans, livestock and animal models. The effects of "soluble" forms of fiber (specifically pectins, gums, resistant starches, lactulose, oligofructose and inulin) on mineral absorption are more difficult to characterize. The addition of these soluble forms of fiber has been found in various studies to add viscosity to the gut contents, promote fermentation and the production of volatile fatty acids in the cecum, have a trophic effect on the ceca of animals and increase serum enteroglucagon concentrations. Thus it is not surprising that the addition of soluble forms of fiber to diets often has been found to improve absorption of minerals. This may reflect absorption of electrolytes from the large intestine. Future work should address the mechanisms by which ingestion of nondigestible carbohydrates improves mineral absorption in humans.

  19. Sink filling, inulin metabolizing enzymes and carbohydrate status in field grown chicory (Cichorium intybus L.).

    NARCIS (Netherlands)

    Arkel, van J.; Vergauwen, R.; Sévenier, R.; Hakkert, J.C.; Laere, van A.; Bouwmeester, H.J.; Meer, van der I.M.

    2012-01-01

    Inulin is a fructose-based polymer that is isolated from chicory (Cichorium intybus L.) taproots. The degree of polymerization (DP) determines its application and hence the value of the crop. The DP is highly dependent on the field conditions and harvest time. Therefore, the present study was carrie

  20. Removal of root filling materials.

    LENUS (Irish Health Repository)

    Duncan, H.F. Chong, B.S.

    2011-05-01

    Safe, successful and effective removal of root filling materials is an integral component of non-surgical root canal re-treatment. Access to the root canal system must be achieved in order to negotiate to the canal terminus so that deficiencies in the original treatment can be rectified. Since a range of materials have been advocated for filling root canals, different techniques are required for their removal. The management of commonly encountered root filling materials during non-surgical re-treatment, including the clinical procedures necessary for removal and the associated risks, are reviewed. As gutta-percha is the most widely used and accepted root filling material, there is a greater emphasis on its removal in this review.

  1. The Growth Analysis in the Kernel Filling Process of K-Type Hybrid 901 and Its Parent Wheat

    Institute of Scientific and Technical Information of China (English)

    GONG Yue-hua; LIU Ying-zhou; GAO Jun-feng

    2005-01-01

    Using Matlab software, the grain filling process of hybrid wheat 901 and its parents was fitted by Richards equation W = A/(1 +Be-kt in order to study the characteristics of grain filling of the hybrid. The active grain growth period of the hybrid was 6 d longer than that of Shaan 229, and its final grain weight (43.7 g/1000 grains) was higher than that of Shaan229 (36.3 g/1 000 grains). N values of 901 and R205 were both less than 1, and their grain growth was faster in the early filling stage, while slower in the middle-late stage. N value of Shaan 229 was >1, and its growth was slower in the early stage and faster in the middle stage. The period of early stage of 901 was shorter and of middle-late stage was longer. The situation of Shaan 229 was totally reversed. For parents, the father plant R205 was similar to hybrid wheat 901, whereas its mother plant K3314A similar to Shaan 229. It has been found that Richards equation was more suitable for fitting the grain filling process of wheat than Logistic equation.

  2. Utilization of carbohydrates by radiation processing

    Energy Technology Data Exchange (ETDEWEB)

    Kume, T. E-mail: kume@taka.jaeri.go.jp; Nagasawa, N.; Yoshii, F

    2002-03-01

    Upgrading and utilization of carbohydrates such as chitosan, sodium alginate, carrageenan, cellulose, pectin have been investigated for recycling these bio-resources and reducing the environmental pollution. These carbohydrates were easily degraded by irradiation and various kinds of biological activities such as anti-microbial activity, promotion of plant growth, suppression of heavy metal stress, phytoalexins induction, etc. were induced. On the other hand, some carbohydrate derivatives, carboxymethylcellulose and carboxymethylstarch, could be crosslinked under certain radiation condition and produce the biodegradable hydrogel for medical and agricultural use.

  3. Carbohydrate functionalized carbon nanotubes and their applications.

    Science.gov (United States)

    Gorityala, Bala Kishan; Ma, Jimei; Wang, Xin; Chen, Peng; Liu, Xue-Wei

    2010-08-01

    Carbon nanotubes (CNTs) have attracted tremendous attention in biomedical applications due to their molecular size and unique properties. This tutorial review summarizes the strategies to functionalize CNTs with bioactive carbohydrates, which improve their solubility, biocompatibility and biofunctionalities while preserving their desired properties. In addition, studies on the usage of carbohydrate functionalized CNTs to detect bacteria, to bind to specific lectins, to deliver glycomimetic drug molecules into cells and to probe cellular activities as biosensors are reviewed. Improvement in biocompatibility and introduction of bio-functionalities by integration of carbohydrate with CNTs are paving the way to glyconanotechnology and may provide new tools for glycobiological studies.

  4. Against the Grain

    DEFF Research Database (Denmark)

    Pedersen, Peter Ole

    2016-01-01

    Katalog-tekst til udstillingen Against the Grain om atomar-fotografi og det antropocæne. Kurateret af Peter Ole Pedersen på Galleri Image, august-oktober 2016.......Katalog-tekst til udstillingen Against the Grain om atomar-fotografi og det antropocæne. Kurateret af Peter Ole Pedersen på Galleri Image, august-oktober 2016....

  5. Granular gases of rod-shaped grains in microgravity.

    Science.gov (United States)

    Harth, K; Kornek, U; Trittel, T; Strachauer, U; Höme, S; Will, K; Stannarius, R

    2013-04-05

    Granular gases are convenient model systems to investigate the statistical physics of nonequilibrium systems. In the literature, one finds numerous theoretical predictions, but only few experiments. We study a weakly excited dilute gas of rods, confined in a cuboid container in microgravity during a suborbital rocket flight. With respect to a gas of spherical grains at comparable filling fraction, the mean free path is considerably reduced. This guarantees a dominance of grain-grain collisions over grain-wall collisions. No clustering was observed, unlike in similar experiments with spherical grains. Rod positions and orientations were determined and tracked. Translational and rotational velocity distributions are non-Gaussian. Equipartition of kinetic energy between translations and rotations is violated.

  6. Carbohydrate feeding and exercise: effect of beverage carbohydrate content.

    Science.gov (United States)

    Murray, R; Seifert, J G; Eddy, D E; Paul, G L; Halaby, G A

    1989-01-01

    The purpose of this study was to determine the effect of ingesting fluids of varying carbohydrate content upon sensory response, physiologic function, and exercise performance during 1.25 h of intermittent cycling in a warm environment (Tdb = 33.4 degrees C). Twelve subjects (7 male, 5 female) completed four separate exercise sessions; each session consisted of three 20 min bouts of cycling at 65% VO2max, with each bout followed by 5 min rest. A timed cycling task (1200 pedal revolutions) completed each exercise session. Immediately prior to the first 20 min cycling bout and during each rest period, subjects consumed 2.5 ml.kg BW-1 of water placebo (WP), or solutions of 6%, 8%, or 10% sucrose with electrolytes (20 mmol.l-1 Na+, 3.2 mmol.l-1 K+). Beverages were administered in double blind, counterbalanced order. Mean (+/- SE) times for the 1200 cycling task differed significantly: WP = 13.62 +/- 0.33 min, *6% = 13.03 +/- 0.24 min, 8% = 13.30 +/- 0.25 min, 10% = 13.57 +/- 0.22 min (* = different from WP and 10%, P less than 0.05). Compared to WP, ingestion of the CHO beverages resulted in higher plasma glucose and insulin concentrations, and higher RER values during the final 20 min of exercise (P less than 0.05). Markers of physiologic function and sensory perception changed similarly throughout exercise; no differences were observed among subjects in response to beverage treatments for changes in plasma concentrations of lactate, sodium, potassium, for changes in plasma volume, plasma osmolality, rectal temperature, heart rate, oxygen uptake, rating of perceived exertion, or for indices of gastrointestinal distress, perceived thirst, and overall beverage acceptance.(ABSTRACT TRUNCATED AT 250 WORDS)

  7. GrainSpotter

    DEFF Research Database (Denmark)

    Schmidt, Søren

    2014-01-01

    A new approach for indexing multigrain diffraction data is presented. It is based on the use of a monochromatic beam simultaneously illuminating all grains. By operating in sub-volumes of Rodrigues space, a powerful vertex-finding algorithm can be applied, with a running time that is compatible...... with online analysis. The resulting program, GrainSpotter, is sufficiently fast to enable online analysis during synchrotron sessions. The program applies outlier rejection schemes, leading to more robust and accurate data. By simulations it is shown that several thousand grains can be retrieved. A new method...... to derive partial symmetries, called pseudo-twins, is introduced. Uniquely, GrainSpotter includes an analysis of pseudo-twins, which is shown to be critical to avoid erroneous grains resulting from the indexing....

  8. Physics of Grain Alignment

    CERN Document Server

    Lazarian, A

    2000-01-01

    Aligned grains provide one of the easiest ways to study magnetic fields in diffuse gas and molecular clouds. How reliable our conclusions about the inferred magnetic field depends critically on our understanding of the physics of grain alignment. Although grain alignment is a problem of half a century standing recent progress achieved in the field makes us believe that we are approaching the solution of this mystery. I review basic physical processes involved in grain alignment and show why mechanisms that were favored for decades do not look so promising right now. I also discuss why the radiative torque mechanism ignored for more than 20 years looks right now the most powerful means of grain alignment.

  9. A Molecular Dynamics Approach to Grain Boundary Structure and Migration

    DEFF Research Database (Denmark)

    Cotterill, R. M. J.; Leffers, Torben; Lilholt, Hans

    1974-01-01

    It has been demonstrated that grain boundary formation from the melt can be simulated by the molecular dynamics method. The space between two mutually-misoriented crystal slabs was filled with atoms in a random manner and this liquid was then cooled until crystallization occurred. The general...

  10. Workshop to establish databases of carbohydrate spectra

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-12-31

    The workshop was organized to formulate guidelines for establishing spectral databases of complex carbohydrates. The databases will enable the scientific community to avoid the great waste of research effort and funds that frequently occurs when carbohydrate chemists are forced to duplicate the structural characterization of previously characterized complex carbohydrates. Chemists waste their effort on repetitive characterizations because in the absence of spectral databases they are unaware they are analyzing a known molecule until they have completely determined its structure. Chemists will be able to avoid much of this wasted effort when the collections of mass and of nuclear magnetic resonance (NMR) spectra initiated at the workshop are subsequently developed into searchable databases. Then scientists only need query the databases with the spectrum or with information defining the spectrum of an unidentified carbohydrate to find out if it has been previously characterized.

  11. Carbohydrates in pig nutrition - Recent advances

    DEFF Research Database (Denmark)

    Knudsen, Knud Erik Bach; Lærke, Helle Nygaard; Ingerslev, Anne Krog

    2016-01-01

    The dietary carbohydrates are a diverse group of substances with a range of chemical, physical, and physiological properties. The primary chemical classification of carbohydrates is by molecular size (degree of polymerization [DP]), the type of linkage (α or β), and composition of individual...... in the small intestine. Some types of soluble NSP are found to interact with intestinal mucus and produce a layer that significantly delays the transport of lipid digestion products. Potentially, the same may be the case for proteinous compounds. The delay in the transport of the nutrients to the gut...... monomers. This approach divides carbohydrates into 3 main groups, sugars (DP1–2), oligosaccharides (DP3–9), and polysaccharides (DP ≥ 10), the latter being further divided into starch (α-1:4,1,6-D-glucans) and nonstarch polysaccharides (NSP). Dietary fiber (DF) recently has been defined as carbohydrate...

  12. Gas-Filled Capillary Model

    Science.gov (United States)

    Steinhauer, L. C.; Kimura, W. D.

    2006-11-01

    We have developed a 1-D, quasi-steady-state numerical model for a gas-filled capillary discharge that is designed to aid in selecting the optimum capillary radius in order to guide a laser beam with the required intensity through the capillary. The model also includes the option for an external solenoid B-field around the capillary, which increases the depth of the parabolic density channel in the capillary, thereby allowing for propagation of smaller laser beam waists. The model has been used to select the parameters for gas-filled capillaries to be utilized during the Staged Electron Laser Acceleration — Laser Wakefield (STELLA-LW) experiment.

  13. QENS investigation of filled rubbers

    Energy Technology Data Exchange (ETDEWEB)

    Triolo, A.; Lechner, R.E.; Desmedt, A.; Pieper, J. [CNR - Istituto per i Processi Chimico-Fisici, sez. Messina, Via La Farina 237, 98123 Messina (Italy); Lo Celso, F.; Triolo, R. [Dip. Chimica Fisica, V. le delle Scienze, Parco d' Orleans, Padiglione 17, Universita di Palermo, 90128 Palermo (Italy); Negroni, F. [Pirelli Pneumatici S.p.A, V. le Sarca 222, 20126 Milano (Italy); Arrighi, V.; Qian, H. [Chemistry School of Engineering and Physical Sciences, Heriot-Watt University, EH14 4AS Edinburgh (United Kingdom); Frick, B. [Institut Laue-Langevin, 6, rue Jules Horowitz, BP 156, 38042 Grenoble, Cedex 9 (France)

    2002-07-01

    The polymer segmental dynamics is investigated in a series of silica-filled rubbers. The presence of inert fillers in polymers greatly affects the mechanical and physical performance of the final materials. For example, silica has been proposed as a reinforcing agent of elastomers in tire production. Results from quasielastic neutron scattering and Dynamic Mechanical Thermal Analysis (DMTA) measurements are presented on styrene-ran-butadiene rubber filled with silica. A clear indication is obtained of the existence of a bimodal dynamics, which can be rationalized in terms of the relaxation of bulk rubber and the much slower relaxation of the rubber adsorbed on the filler surface. (orig.)

  14. QENS investigation of filled rubbers

    CERN Document Server

    Triolo, A; Desmedt, A; Pieper, J K; Lo Celso, F; Triolo, R; Negroni, F; Arrighi, V; Qian, H; Frick, B

    2002-01-01

    The polymer segmental dynamics is investigated in a series of silica-filled rubbers. The presence of inert fillers in polymers greatly affects the mechanical and physical performance of the final materials. For example, silica has been proposed as a reinforcing agent of elastomers in tire production. Results from quasielastic neutron scattering and Dynamic Mechanical Thermal Analysis (DMTA) measurements are presented on styrene-ran-butadiene rubber filled with silica. A clear indication is obtained of the existence of a bimodal dynamics, which can be rationalized in terms of the relaxation of bulk rubber and the much slower relaxation of the rubber adsorbed on the filler surface. (orig.)

  15. QENS investigation of filled rubbers

    Science.gov (United States)

    Triolo, A.; Lo Celso, F.; Negroni, F.; Arrighi, V.; Qian, H.; Lechner, R. E.; Desmedt, A.; Pieper, J.; Frick, B.; Triolo, R.

    The polymer segmental dynamics is investigated in a series of silica-filled rubbers. The presence of inert fillers in polymers greatly affects the mechanical and physical performance of the final materials. For example, silica has been proposed as a reinforcing agent of elastomers in tire production. Results from quasielastic neutron scattering and Dynamic Mechanical Thermal Analysis (DMTA) measurements are presented on styrene-ran-butadiene rubber filled with silica. A clear indication is obtained of the existence of a bimodal dynamics, which can be rationalized in terms of the relaxation of bulk rubber and the much slower relaxation of the rubber adsorbed on the filler surface.

  16. Grain size effects on He bubbles distribution and evolution

    Energy Technology Data Exchange (ETDEWEB)

    Wang, J. [Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000 (China); University of Chinese Academy of Sciences, Beijing 100049 (China); School of Physical Science and Technology, Lanzhou University, Lanzhou 730000 (China); Gao, X.; Gao, N. [Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000 (China); Wang, Z.G., E-mail: zhgwang@impcas.ac.cn [Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000 (China); Cui, M.H.; Wei, K.F.; Yao, C.F.; Sun, J.R.; Li, B.S.; Zhu, Y.B.; Pang, L.L. [Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000 (China); Li, Y.F. [Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000 (China); University of Chinese Academy of Sciences, Beijing 100049 (China); School of Physical Science and Technology, Lanzhou University, Lanzhou 730000 (China); Wang, D. [Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000 (China); University of Chinese Academy of Sciences, Beijing 100049 (China); Xie, E.Q. [School of Physical Science and Technology, Lanzhou University, Lanzhou 730000 (China)

    2015-02-15

    Highlights: • SMAT treated T91 and conventional T91 were implanted by 200 keV He{sup 2+} to 1 × 10{sup 21} He m{sup −2} at room temperature and annealed at 450 °C for 3.5 h. • He bubbles in nanometer-size-grained T91 are smaller in as-implanted case. • The bubbles in the matrix of nanograins were hard to detect and those along the nanograin boundaries coalesced and filled with the grain boundaries after annealing. • Brownian motion and coalescence and Ostwald ripening process might lead to bubbles morphology presented in the nanometer-size-grained T91 after annealing. - Abstract: Grain boundary and grain size effects on He bubble distribution and evolution were investigated by He implantation into nanometer-size-grained T91 obtained by Surface Mechanical Attrition Treatment (SMAT) and the conventional coarse-grained T91. It was found that bubbles in the nanometer-size-grained T91 were smaller than those in the conventional coarse-grained T91 in as-implanted case, and bubbles in the matrix of nanograins were undetectable while those at nanograin boundaries (GBs) coalesced and filled in GBs after heat treatment. These results suggested that the grain size of structural material should be larger than the mean free path of bubble’s Brownian motion and/or denuded zone around GBs in order to prevent bubbles accumulation at GBs, and multiple instead of one type of defects should be introduced into structural materials to effectively reduce the susceptibility of materials to He embrittlement and improve the irradiation tolerance of structural materials.

  17. The Origin of the Constant Carbohydrate Diet

    Directory of Open Access Journals (Sweden)

    Charles Herbert Read

    2009-01-01

    Full Text Available The Constant Carbohydrate diet, based entirely on carbohydrate exchanges, is now widely used in the dietary treatment of diabetes mellitus. Being based on sound scientific principles and simple in design, the Constant Carabohydrate diet is appropriate for all those having diabetes mellitus, young or old, no matter their ethncity. This report describes why and how it was developed in 1951. Its simplicity makes it adaptable to all ethnic diets.

  18. The Origin of the Constant Carbohydrate Diet

    Directory of Open Access Journals (Sweden)

    Read CharlesHerbert

    2008-01-01

    Full Text Available The Constant Carbohydrate diet, based entirely on carbohydrate exchanges, is now widely used in the dietary treatment of diabetes mellitus. Being based on sound scientific principles and simple in design, the Constant Carabohydrate diet is appropriate for all those having diabetes mellitus, young or old, no matter their ethncity. This report describes why and how it was developed in 1951. Its simplicity makes it adaptable to all ethnic diets.

  19. Conversion of carbohydrates to levulinic acid esters

    DEFF Research Database (Denmark)

    2014-01-01

    The present invention relates to the field of converting carbohydrates into levulinic acid, a platform chemical for many chemical end products. More specifically the invention relates to a method for converting carbohydrates such as mono-, di- or polysaccharides, obtained from for example biomass...... production into a suitable levulinic acid ester in the presence of a zeolite or zeotype catalyst and a suitable alcohol, and the ester may be further converted into levulinic acid if desired....

  20. Relationship Between Root Chemical Signals and Grain Quality of Rice

    Institute of Scientific and Technical Information of China (English)

    YANG Jian-chang; CHANG Er-hua; ZHANG Wen-jie; WANG Zhi-qin; LIU Li-jun

    2007-01-01

    This study was designed to elucidate the relationship between root chemical signals and the quality of rice. Various rice genotypes were used. Zeatin (Z) + zeatin riboside (ZR), abscisic acid (ABA), 1-aminocylopropane -1-carboxylic acid (ACC), and organic acids in roots during grain filling and the appearance quality, cooking/eating quality were investigated. The correlations among them were analyzed. The results showed that Z + ZR concentrations in the roots at mid- and lategrain-filling stages were significantly and positively correlated with the gel consistency and alkali spreading value (r = 0.72* - 0.90**), whereas negatively correlated with the amylose content (r = -0.68* - -0.78**). ABA concentrations in roots at mid- grain-filling stage were significantly and negatively correlated with the gel consistency and alkali spreading value (r = -0.90** - -0.91**), and positively correlated with the amylose content (r = 0.87**). ACC concentrations in root exudates at mid-grain-filling stage were very significantly correlated with the percentage of chalky grains and chalkiness (r = 0.97** -0.98**), and those at late-grain-filling stage Were significantly correlated with chalkiness and chalky size (r = 0.69*-0.96**). The more the malic acid and succinic acid exuded from roots for a cultivar, the greater the breakdown values and the smaller the setback values in the starch profile, and the results were reversed for a cultivar with more tartaric acid and citric acid exuded from roots during the grain-filling period. The cultivar with more lactic acid in exudates had smaller gel consistency and alkali spreading values, but had greater amylose content. When roots were treated with exogenous ZR, ABA, and ACC during grain filling, effects of the chemicals on the rice quality were consistent with the relationships of the endogenous hormones (Z + ZR, ABA, and ACC) with the quality indexes. Using rape cake as organic fertilizer can increase the concentrations of malic acid and

  1. Molecular simulations of carbohydrates and protein-carbohydrate interactions: motivation, issues and prospects.

    Science.gov (United States)

    Fadda, Elisa; Woods, Robert J

    2010-08-01

    The characterization of the 3D structure of oligosaccharides, their conjugates and analogs is particularly challenging for traditional experimental methods. Molecular simulation methods provide a basis for interpreting sparse experimental data and for independently predicting conformational and dynamic properties of glycans. Here, we summarize and analyze the issues associated with modeling carbohydrates, with a detailed discussion of four of the most recently developed carbohydrate force fields, reviewed in terms of applicability to natural glycans, carbohydrate-protein complexes and the emerging area of glycomimetic drugs. In addition, we discuss prospectives and new applications of carbohydrate modeling in drug discovery.

  2. Filling of charged cylindrical capillaries

    NARCIS (Netherlands)

    Das, Siddhartha; Chanda, Sourayon; Eijkel, J.C.T.; Tas, N.R.; Chakraborty, Suman; Mitra, Sushanta K.

    2014-01-01

    We provide an analytical model to describe the filling dynamics of horizontal cylindrical capillaries having charged walls. The presence of surface charge leads to two distinct effects: It leads to a retarding electrical force on the liquid column and also causes a reduced viscous drag force because

  3. Filling in the retinal image

    Science.gov (United States)

    Larimer, James; Piantanida, Thomas

    1990-01-01

    The optics of the eye form an image on a surface at the back of the eyeball called the retina. The retina contains the photoreceptors that sample the image and convert it into a neural signal. The spacing of the photoreceptors in the retina is not uniform and varies with retinal locus. The central retinal field, called the macula, is densely packed with photoreceptors. The packing density falls off rapidly as a function of retinal eccentricity with respect to the macular region and there are regions in which there are no photoreceptors at all. The retinal regions without photoreceptors are called blind spots or scotomas. The neural transformations which convert retinal image signals into percepts fills in the gaps and regularizes the inhomogeneities of the retinal photoreceptor sampling mosaic. The filling-in mechamism plays an important role in understanding visual performance. The filling-in mechanism is not well understood. A systematic collaborative research program at the Ames Research Center and SRI in Menlo Park, California, was designed to explore this mechanism. It was shown that the perceived fields which are in fact different from the image on the retina due to filling-in, control some aspects of performance and not others. Researchers have linked these mechanisms to putative mechanisms of color coding and color constancy.

  4. Unsteady Capillary Filling By Electrocapillarity

    Science.gov (United States)

    Kang, In Seok; Lee, Jung A.

    2016-11-01

    Unsteady filling of electrolyte solution inside a nanochannel by the electrocapillarity effect is studied. The filling rate is predicted as a function of the bulk concentration of the electrolyte, the surface potential (or surface charge density), and the cross sectional shape of the channel. Since the driving force of the flow is the electrocapillarity, it is first analyzed by using the solution of the Poisson-Boltzmann equation. From the analysis, it is found that the results for many different cross sectional shapes can be unified with good accuracy if the hydraulic radius is adopted as the characteristic length scale of the problem. Especially in the case of constant surface potential, for both limits of κh -> 0 and κh -> ∞ , it can be shown theoretically that the electrocapillarity is independent of the cross sectional shape if the hydraulic radius is the same. In order to analyze the geometric effects more systematically, we consider the regular N-polygons with the same hydraulic radius and the rectangles of different aspect ratios. Washburn's approach is then adopted to predict the filling rate of electrolyte solution inside a nanaochannel. It is found that the average filling velocity decreases as N increases in the case of regular N-polygons with the same hydraulic radius. This is because of that the regular N-polygons of the same hydraulic radius share the same inscribing circle. This work has been supported by BK21+ program.

  5. Space-filling polyhedral sorbents

    Science.gov (United States)

    Haaland, Peter

    2016-06-21

    Solid sorbents, systems, and methods for pumping, storage, and purification of gases are disclosed. They derive from the dynamics of porous and free convection for specific gas/sorbent combinations and use space filling polyhedral microliths with facial aplanarities to produce sorbent arrays with interpenetrating interstitial manifolds of voids.

  6. Brewing Beer in the Laboratory: Grain Amylases and Yeast's Sweet Tooth

    Science.gov (United States)

    Gillespie, Blake; Deutschman, William A.

    2010-01-01

    Brewing beer provides a straightforward and robust laboratory counterpart to classroom discussions of fermentation, a staple of the biochemistry curriculum. An exercise is described that provides several connections between lecture and laboratory content. Students first extract fermentable carbohydrates from whole grains, then ferment these with…

  7. Beyond whole grain: The European HEALTHGRAIN project aims at healthier cereal foods

    NARCIS (Netherlands)

    Poutanen, K.; Shepherd, R.; Shewry, P.R.; Delcour, J.A.; Björck, I.; Kamp, J.W. van der

    2008-01-01

    Cereal foods are an important source of carbohydrates and dietary fiber in our diet. Epidemiological evidence increasingly demonstrates that a diet rich in whole grain is protective against development of diet-related disorders such as cardiovascular disease and type 2 diabetes. The majority of cere

  8. Grain Boundary Complexions

    Science.gov (United States)

    2014-05-01

    theories of grain boundary adsorption were based on the McLean model, which assumes that segregation is constrained to a monolayer of atomic sites at the...New York: McGraw- Hill ; 1956. [58] de Boer JH. The dynamical character of adsorption . London: Oxford University Press; 1953. [59] McLean D. Grain...Overview No. 152 Grain boundary complexions Patrick R. Cantwell a, Ming Tang b,1, Shen J. Dillon c, Jian Luo d, Gregory S . Rohrer e, Martin P. Harmer

  9. Folding of Pollen Grains

    Science.gov (United States)

    Katifori, Eleni; Alben, Silas; Cerda, Enrique; Nelson, David; Dumais, Jacques

    2008-03-01

    At dehiscence, which occurs when the anther reaches maturity and opens, pollen grains dehydrate and their volume is reduced. The pollen wall deforms to accommodate the volume loss, and the deformation pathway depends on the initial turgid pollen grain geometry and the mechanical properties of the pollen wall. We demonstrate, using both experimental and theoretical approaches, that the design of the apertures (areas on the pollen wall where the stretching and the bending modulus are reduced) is critical for controlling the folding pattern, and ensures the pollen grain viability. An excellent fit to the experiments is obtained using a discretized version of the theory of thin elastic shells.

  10. Characterization of protein and carbohydrate mid-IR spectral features in crop residues

    Science.gov (United States)

    Xin, Hangshu; Zhang, Yonggen; Wang, Mingjun; Li, Zhongyu; Wang, Zhibo; Yu, Peiqiang

    2014-08-01

    To the best of our knowledge, a few studies have been conducted on inherent structure spectral traits related to biopolymers of crop residues. The objective of this study was to characterize protein and carbohydrate structure spectral features of three field crop residues (rice straw, wheat straw and millet straw) in comparison with two crop vines (peanut vine and pea vine) by using Fourier transform infrared spectroscopy (FTIR) technique with attenuated total reflectance (ATR). Also, multivariate analyses were performed on spectral data sets within the regions mainly related to protein and carbohydrate in this study. The results showed that spectral differences existed in mid-IR peak intensities that are mainly related to protein and carbohydrate among these crop residue samples. With regard to protein spectral profile, peanut vine showed the greatest mid-IR band intensities that are related to protein amide and protein secondary structures, followed by pea vine and the rest three field crop straws. The crop vines had 48-134% higher spectral band intensity than the grain straws in spectral features associated with protein. Similar trends were also found in the bands that are mainly related to structural carbohydrates (such as cellulosic compounds). However, the field crop residues had higher peak intensity in total carbohydrates region than the crop vines. Furthermore, spectral ratios varied among the residue samples, indicating that these five crop residues had different internal structural conformation. However, multivariate spectral analyses showed that structural similarities still exhibited among crop residues in the regions associated with protein biopolymers and carbohydrate. Further study is needed to find out whether there is any relationship between spectroscopic information and nutrition supply in various kinds of crop residue when fed to animals.

  11. Detecting molecular features of spectra mainly associated with structural and non-structural carbohydrates in co-products from bioEthanol production using DRIFT with uni- and multivariate molecular spectral analyses.

    Science.gov (United States)

    Yu, Peiqiang; Damiran, Daalkhaijav; Azarfar, Arash; Niu, Zhiyuan

    2011-01-01

    The objective of this study was to use DRIFT spectroscopy with uni- and multivariate molecular spectral analyses as a novel approach to detect molecular features of spectra mainly associated with carbohydrate in the co-products (wheat DDGS, corn DDGS, blend DDGS) from bioethanol processing in comparison with original feedstock (wheat (Triticum), corn (Zea mays)). The carbohydrates related molecular spectral bands included: A_Cell (structural carbohydrates, peaks area region and baseline: ca. 1485-1188 cm(-1)), A_1240 (structural carbohydrates, peak area centered at ca. 1240 cm(-1) with region and baseline: ca. 1292-1198 cm(-1)), A_CHO (total carbohydrates, peaks region and baseline: ca. 1187-950 cm(-1)), A_928 (non-structural carbohydrates, peak area centered at ca. 928 cm(-1) with region and baseline: ca. 952-910 cm(-1)), A_860 (non-structural carbohydrates, peak area centered at ca. 860 cm(-1) with region and baseline: ca. 880-827 cm(-1)), H_1415 (structural carbohydrate, peak height centered at ca. 1415 cm(-1) with baseline: ca. 1485-1188 cm(-1)), H_1370 (structural carbohydrate, peak height at ca. 1370 cm(-1) with a baseline: ca. 1485-1188 cm(-1)). The study shows that the grains had lower spectral intensity (KM Unit) of the cellulosic compounds of A_1240 (8.5 vs. 36.6, P carbohydrate of A_928 (17.3 vs. 2.0) and A_860 (20.7 vs. 7.6) than their co-products from bioethanol processing. There were no differences (P > 0.05) in the peak area intensities of A_Cell (structural CHO) at 1292-1198 cm(-1) and A_CHO (total CHO) at 1187-950 cm(-1) with average molecular infrared intensity KM unit of 226.8 and 508.1, respectively. There were no differences (P > 0.05) in the peak height intensities of H_1415 and H_1370 (structural CHOs) with average intensities 1.35 and 1.15, respectively. The multivariate molecular spectral analyses were able to discriminate and classify between the corn and corn DDGS molecular spectra, but not wheat and wheat DDGS. This study indicated that

  12. Metabolic aspects of low carbohydrate diets and exercise

    OpenAIRE

    Peters Sandra; LeBlanc Paul

    2004-01-01

    Abstract Following a low carbohydrate diet, there is a shift towards more fat and less carbohydrate oxidation to provide energy to skeletal muscle, both at rest and during exercise. This review summarizes recent work on human skeletal muscle carbohydrate and fat metabolic adaptations to a low carbohydrate diet, focusing mainly on pyruvate dehydrogenase and pyruvate dehydrogenase kinase, and how these changes relate to the capacity for carbohydrate oxidation during exercise.

  13. Metabolic aspects of low carbohydrate diets and exercise

    Directory of Open Access Journals (Sweden)

    Peters Sandra

    2004-01-01

    Full Text Available Abstract Following a low carbohydrate diet, there is a shift towards more fat and less carbohydrate oxidation to provide energy to skeletal muscle, both at rest and during exercise. This review summarizes recent work on human skeletal muscle carbohydrate and fat metabolic adaptations to a low carbohydrate diet, focusing mainly on pyruvate dehydrogenase and pyruvate dehydrogenase kinase, and how these changes relate to the capacity for carbohydrate oxidation during exercise.

  14. Incorporation of whole, ancient grains into a modern Asian Indian diet to reduce the burden of chronic disease.

    Science.gov (United States)

    Dixit, Anjali A; Azar, Kristen Mj; Gardner, Christopher D; Palaniappan, Latha P

    2011-08-01

    Refined carbohydrates, such as white rice and white flour, are the mainstay of the modern Asian Indian diet, and may contribute to the rising incidence of type 2 diabetes and cardiovascular disease in this population. Prior to the 1950s, whole grains such as amaranth, barley, brown rice, millet, and sorghum were more commonly used in Asian Indian cooking. These grains and other non-Indian grains such as couscous, quinoa, and spelt are nutritionally advantageous and may be culturally acceptable carbohydrate substitutes for Asian Indians. This review focuses on practical recommendations for culturally sensitive carbohydrate modification in a modern Asian Indian diet to reduce type 2 diabetes and cardiovascular disease in this population.

  15. Shearing fluid-filled granular media: A coupled discrete element - continuous approach

    Science.gov (United States)

    Goren, L.; Aharonov, E.; Sparks, D.; Toussaint, R.; Marder, E.

    2012-04-01

    Fluid-filled granular layers are abundant in the Earth's shallow crust as saturated soils and poorly consolidated hillslope material, and as fluid-filled fault gouge layers. When such grains-fluid systems are subjected to excitation by the passage of seismic waves, tectonic loading, or gravitational loading they exhibit a highly non-trivial dynamical behavior that may lead to instabilities in the form of soil liquefaction, debris flow mobilization, and earthquakes. In order to study the basic coupled mechanics of fluid-filled granular media and the dynamical processes that are responsible for the emergence of instabilities we develop a model that couples granular dynamics (DEM) algorithm with a continuous Eulerian grid-based solver. The two components of the model represent the two phases (grains and fluid) in two different scales. Each grain is represented by a single element in the granular dynamics component, where grains interact by elastic collisions and frictional sliding. The compressible pore fluid is represented on a coarser Darcy scale grid that is super-imposed over the grains layer. The pore space geometry set by the evolving granular packing is used to define smooth porosity and permeability fields, and the individual grain velocities are interpolated to define a smooth field of a solid-fraction velocity. The porosity, permeability, and solid velocity fields are used in the continuous fluid grid-based solver to find pore fluid velocity and pressure. Pore fluid pressure gradients are interpolated back from the fluid grid to individual grains, where they enter the grains force balance equation as seepage forces. Boundary conditions are specified separately for the two phases. For the pore fluid we test two end-member drainage conditions: completely drained system (with infinite boundary permeability) and completely undrained system (with zero boundary permeability). For the grains, two-dimensional time dependent stress and velocity conditions are

  16. Potential effect of ultrasound on carbohydrates.

    Science.gov (United States)

    Bera, Smritilekha; Mondal, Dhananjoy; Martin, Jacob T; Singh, Man

    2015-06-17

    The use of ultrasound has emerged as one of the most useful alternative energy sources for the synthesis of carbohydrate-derived biologically and pharmaceutically potential compounds. Spectacular advances have been made in the field of sonication-assisted organic reactions, which are known for producing superior yields, enhanced reactivity of the reactant, improved stereoselectivity, and shortened reaction times. Orthogonal protection-deprotection reactions and/or modification and manipulation of functional groups in carbohydrates are common synthetic steps in carbohydrate chemistry. These reaction steps can be driven by the ultrasonic energy generated by acoustic cavitation via the formation and subsequent collapse of ultrasound-induced bubbles. The ultrasound-assisted synthesis of differently functionalised monosaccharides is useful in a wide variety of applications of carbohydrate chemistry such as the glycosylation of oligosaccharides, one pot domino reactions, thioglycoside syntheses, azidoglycoside syntheses, 1,3-dipolar cycloaddition reactions, and syntheses of natural products. This review article covers ultrasound-mediated reactions on carbohydrates that have been described in the literature since 2000.

  17. Saturated fat, carbohydrate, and cardiovascular disease.

    Science.gov (United States)

    Siri-Tarino, Patty W; Sun, Qi; Hu, Frank B; Krauss, Ronald M

    2010-03-01

    A focus of dietary recommendations for cardiovascular disease (CVD) prevention and treatment has been a reduction in saturated fat intake, primarily as a means of lowering LDL-cholesterol concentrations. However, the evidence that supports a reduction in saturated fat intake must be evaluated in the context of replacement by other macronutrients. Clinical trials that replaced saturated fat with polyunsaturated fat have generally shown a reduction in CVD events, although several studies showed no effects. An independent association of saturated fat intake with CVD risk has not been consistently shown in prospective epidemiologic studies, although some have provided evidence of an increased risk in young individuals and in women. Replacement of saturated fat by polyunsaturated or monounsaturated fat lowers both LDL and HDL cholesterol. However, replacement with a higher carbohydrate intake, particularly refined carbohydrate, can exacerbate the atherogenic dyslipidemia associated with insulin resistance and obesity that includes increased triglycerides, small LDL particles, and reduced HDL cholesterol. In summary, although substitution of dietary polyunsaturated fat for saturated fat has been shown to lower CVD risk, there are few epidemiologic or clinical trial data to support a benefit of replacing saturated fat with carbohydrate. Furthermore, particularly given the differential effects of dietary saturated fats and carbohydrates on concentrations of larger and smaller LDL particles, respectively, dietary efforts to improve the increasing burden of CVD risk associated with atherogenic dyslipidemia should primarily emphasize the limitation of refined carbohydrate intakes and a reduction in excess adiposity.

  18. Carbohydrates and T cells: a sweet twosome.

    Science.gov (United States)

    Avci, Fikri Y; Li, Xiangming; Tsuji, Moriya; Kasper, Dennis L

    2013-04-01

    Carbohydrates as T cell-activating antigens have been generating significant interest. For many years, carbohydrates were thought of as T-independent antigens, however, more recent research had demonstrated that mono- or oligosaccharides glycosidically linked to peptides can be recognized by T cells. T cell recognition of these glycopeptides depends on the structure of both peptide and glycan portions of the antigen. Subsequently, it was discovered that natural killer T cells recognized glycolipids when presented by the antigen presenting molecule CD1d. A transformative insight into glycan-recognition by T cells occurred when zwitterionic polysaccharides were discovered to bind to and be presented by MHCII to CD4+ T cells. Based on this latter observation, the role that carbohydrate epitopes generated from glycoconjugate vaccines had in activating helper T cells was explored and it was found that these epitopes are presented to specific carbohydrate recognizing T cells through a unique mechanism. Here we review the key interactions between carbohydrate antigens and the adaptive immune system at the molecular, cellular and systems levels exploring the significant biological implications in health and disease.

  19. Analysis of EBSD Grain Size Measurements Using Microstructure Simulations and a Customizable Pattern Matching Library for Grain Perimeter Estimation

    Science.gov (United States)

    Coutinho, Y. A.; Rooney, S. C. K.; Payton, E. J.

    2017-03-01

    Grain size data from electron backscatter diffraction (EBSD) maps are often reported as the mean of the circle equivalent diameters of the measured grain areas. Circle equivalent diameters are not directly comparable to the lineal intercept measurements more historically common for grain size characterization in analog optical microscopy. While the value of mean lineal intercept is the same in 2D and 3D for a given probe direction, the mean 2D circle equivalent section diameter is not directly related to any 3D property. Estimation of mean lineal intercept from circle equivalent diameter is usually carried out by again assuming feature circularity, despite the obvious corners that are inherent to grains from the requirements of space filling. A direct conversion between section areas and lineal intercepts can be performed if the grain perimeters are known. In the present work, a novel pattern matching library approach is investigated for measurement of grain perimeters using simulated 2D EBSD maps. The results are compared to alternative approaches for perimeter measurement and assessed with respect to spatial resolution, grain size distribution parameters, and relevant ASTM and ISO measurement standards. The benefits and drawbacks of each approach are discussed. Empirical estimators for conversion between lineal intercept, circle equivalent diameter, and ASTM grain size number are presented.

  20. Fingering phenomena during grain-grain displacement

    Science.gov (United States)

    Mello, Nathália M. P.; Paiva, Humberto A.; Combe, G.; Atman, A. P. F.

    2016-05-01

    Spontaneous formation of fingered patterns during the displacement of dense granular assemblies was experimentally reported few years ago, in a radial Hele-Shaw cell. Here, by means of discrete element simulations, we have recovered the experimental findings and extended the original study to explore the control parameters space. In particular, using assemblies of grains with different geometries (monodisperse, bidisperse, or polydisperse), we measured the macroscopic stress tensor in the samples in order to confirm some conjectures proposed in analogy with Saffman-Taylor viscous fingering phenomena for immiscible fluids. Considering an axial setup which allows to control the discharge of grains and to follow the trajectory and the pressure gradient along the displacing interface, we have applied the Darcy law for laminar flow in fluids in order to measure an "effective viscosity" for each assembly combination, in an attempt to mimic variation of the viscosity ratio between the injected/displaced fluids in the Saffman-Taylor experiment. The results corroborate the analogy with the viscous fluids displacement, with the bidisperse assembly corresponding to the less viscous geometry. But, differently to fluid case, granular fingers only develop for a specific combination of displaced/injected geometries, and we have demonstrated that it is always related with the formation of a force chain network along the finger direction.

  1. Boronic acids for fluorescence imaging of carbohydrates.

    Science.gov (United States)

    Sun, Xiaolong; Zhai, Wenlei; Fossey, John S; James, Tony D

    2016-02-28

    "Fluorescence imaging" is a particularly exciting and rapidly developing area of research; the annual number of publications in the area has increased ten-fold over the last decade. The rapid increase of interest in fluorescence imaging will necessitate the development of an increasing number of molecular receptors and binding agents in order to meet the demand in this rapidly expanding area. Carbohydrate biomarkers are particularly important targets for fluorescence imaging given their pivotal role in numerous important biological events, including the development and progression of many diseases. Therefore, the development of new fluorescent receptors and binding agents for carbohydrates is and will be increasing in demand. This review highlights the development of fluorescence imaging agents based on boronic acids a particularly promising class of receptors given their strong and selective binding with carbohydrates in aqueous media.

  2. Nutraceutical and pharmacological implications of marine carbohydrates.

    Science.gov (United States)

    Pallela, Ramjee

    2014-01-01

    Current day's research has been focusing much on the potential pharmacological or nutraceutical agents of selective health benefits with less toxicity. As a consequence of increased demand of nutritional supplements of great medicinal values, development of therapeutic agents from natural sources, in particular, marine environment are being considered much important. A diverse array of marine natural products containing medicinally useful nutritional substances, i.e., marine nutraceuticals have been focused to the benefit of mankind. Carbohydrates, by being constituted in considerable amount of many marine organisms display several nutraceutical and pharmaceutical behavior to defend from various diseases. Moreover, the carbohydrates from algae as well as from shellfish wastes, like chitosan and its derivatives, showed tremendous applications in biology and biomedicine. In the current chapter, several of marine carbohydrates from various marine flora and fauna have been covered with their applications and prospects in the development of nutraceuticals and pharmaceuticals.

  3. Investigation of Carbohydrate Recognition via Computer Simulation

    Directory of Open Access Journals (Sweden)

    Quentin R. Johnson

    2015-04-01

    Full Text Available Carbohydrate recognition by proteins, such as lectins and other (biomolecules, can be essential for many biological functions. Recently, interest has arisen due to potential protein and drug design and future bioengineering applications. A quantitative measurement of carbohydrate-protein interaction is thus important for the full characterization of sugar recognition. We focus on the aspect of utilizing computer simulations and biophysical models to evaluate the strength and specificity of carbohydrate recognition in this review. With increasing computational resources, better algorithms and refined modeling parameters, using state-of-the-art supercomputers to calculate the strength of the interaction between molecules has become increasingly mainstream. We review the current state of this technique and its successful applications for studying protein-sugar interactions in recent years.

  4. Sample preparation for the analysis of complex carbohydrates by multicapillary gel electrophoresis with light-emitting diode induced fluorescence detection.

    Science.gov (United States)

    Olajos, Marcell; Hajós, Péter; Bonn, Guenther K; Guttman, András

    2008-06-01

    This paper evaluates various sample preparation methods for multicapillary gel electrophoresis based glycan analysis to support electrokinetic injection. First the removal of excess derivatization reagent is discussed. Although the Sephadex G10 filled multiscreen 96-well filter plate and Sephadex G10 filled pipet tips enabled increased analysis sensitivity, polyamide DPA-6S pipet tips worked particularly well. In this latter case an automated liquid handling system was used to increase purification throughput, necessary to feed the multicapillary electrophoresis unit. Problems associated with the high glucose content of such biological samples as normal human plasma were solved by applying ultrafiltration. Finally, a volatile buffer system was developed for exoglycosidase-based carbohydrate analysis.

  5. Capillary electrophoresis-mass spectrometry of carbohydrates.

    Science.gov (United States)

    Zaia, Joseph

    2013-01-01

    The development of methods for capillary electrophoresis (CE) with on-line mass spectrometric detection (CE/MS) is driven by the need for accurate, robust, and sensitive glycomics analysis for basic biomedicine, biomarker discovery, and analysis of recombinant protein therapeutics. One important capability is to profile glycan mixtures with respect to the patterns of substituents including sialic acids, acetate, sulfate, phosphate, and other groups. There is additional need for an MS-compatible separation system capable of resolving carbohydrate isomers. This chapter summarizes applications of CS/MS to analysis of carbohydrates, glycoproteins, and glycopeptides that have appeared since 2008. Readers are referred to recent comprehensive reviews covering earlier publications.

  6. Synthesis of chiral dopants based on carbohydrates.

    Science.gov (United States)

    Tsuruta, Toru; Koyama, Tetsuo; Yasutake, Mikio; Hatano, Ken; Matsuoka, Koji

    2014-07-01

    Chiral dopants based on carbohydrates for nematic liquid crystals were synthesized from D-glucose, and their helical twisting power (HTP) values were evaluated. The chiral dopants induced helices in the host nematic liquid crystals. An acetyl derivative having an ether-type glycosidic linkage between carbohydrate and a mesogenic moiety showed the highest HTP value of 10.4 μm(-1), while an acetyl derivative having an anomeric ester-type linkage did not show any HTP. It was surprising that this molecule had no HTP despite the presence of chirality in the molecule. A relationship between HTP and specific rotation was not observed in this study.

  7. Composite circumstellar dust grains

    Science.gov (United States)

    Gupta, Ranjan; Vaidya, Dipak B.; Dutta, Rajeshwari

    2016-10-01

    We calculate the absorption efficiencies of composite silicate grains with inclusions of graphite and silicon carbide in the spectral range 5-25 μm. We study the variation in absorption profiles with volume fractions of inclusions. In particular we study the variation in the wavelength of peak absorption at 10 and 18 μm. We also study the variation of the absorption of porous silicate grains. We use the absorption efficiencies to calculate the infrared flux at various dust temperatures and compare with the observed infrared emission flux from the circumstellar dust around some M-type and asymptotic giant branch stars obtained from IRAS and a few stars from Spitzer satellite. We interpret the observed data in terms of the circumstellar dust grain sizes, shape, composition and dust temperature.

  8. Composite Circumstellar Dust Grains

    CERN Document Server

    Gupta, Ranjan; Dutta, Rajeshwari

    2016-01-01

    We calculate the absorption efficiencies of composite silicate grains with inclusions of graphite and silicon carbide in the spectral range 5--25$\\rm \\mu m$. We study the variation in absorption profiles with volume fractions of inclusions. In particular we study the variation in the wavelength of peak absorption at 10 and 18$\\rm \\mu m$. We also study the variation of the absorption of porous silicate grains. We use the absorption efficiencies to calculate the infrared flux at various dust temperatures and compare with the observed infrared emission flux from the circumstellar dust around some M-Type \\& AGB stars obtained from IRAS and a few stars from Spitzer satellite. We interpret the observed data in terms of the circumstellar dust grain sizes; shape; composition and dust temperature.

  9. Approaches to achieve high grain yield and high resource use efficiency in rice

    Directory of Open Access Journals (Sweden)

    Jianchang YANG

    2015-06-01

    Full Text Available This article discusses approaches to simultaneously increase grain yield and resource use efficiency in rice. Breeding nitrogen efficient cultivars without sacrificing rice yield potential, improving grain fill in later-flowering inferior spikelets and enhancing harvest index are three important approaches to achieving the dual goal of high grain yield and high resource use efficiency. Deeper root distribution and higher leaf photosynthetic N use efficiency at lower N rates could be used as selection criteria to develop N-efficient cultivars. Enhancing sink activity through increasing sugar-spikelet ratio at the heading time and enhancing the conversion efficiency from sucrose to starch though increasing the ratio of abscisic acid to ethylene in grains during grain fill could effectively improve grain fill in inferior spikelets. Several practices, such as post-anthesis controlled soil drying, an alternate wetting and moderate soil drying regime during the whole growing season, and non-flooded straw mulching cultivation, could substantially increase grain yield and water use efficiency, mainly via enhanced remobilization of stored carbon from vegetative tissues to grains and improved harvest index. Further research is needed to understand synergistic interaction between water and N on crop and soil and the mechanism underlying high resource use efficiency in high-yielding rice.

  10. Trends in dietary carbohydrate consumption from 1991 to 2008 in the Framingham Heart Study Offspring Cohort.

    Science.gov (United States)

    Makarem, Nour; Scott, Marc; Quatromoni, Paula; Jacques, Paul; Parekh, Niyati

    2014-06-14

    The intake of carbohydrates has been evaluated cross-sectionally, but not longitudinally in an ageing American adult population. The aim of the present study was to examine trends in the intake of dietary carbohydrates and their major food sources among the Framingham Heart Study Offspring (FOS) cohort, which had been uniquely tracked for 17 years in the study. The FOS cohort was recruited in 1971-1975. Follow-up examinations were conducted, on average, every 4 years. Dietary data collection began in 1991 (examination 5) using a validated semi-quantitative FFQ. The study included 2894 adults aged ≥ 25 years with complete dietary data in at least three examinations from 1991 to 2008. Descriptive statistics were generated using SAS version 9.3, and a repeated-measures model was used to examine trends in the intake of carbohydrates and their food sources in the whole sample, and by sex and BMI category. Over 17 years of follow-up, the percentage of energy from total carbohydrates (51·0-46·8 %; P for trend soda, cakes/cookies/quick breads/doughnuts, potatoes, milk, pasta, rice and cooked grains, fruit juice/drinks, potato chips/maize chips/popcorn, and lunch foods (e.g. pizzas and burgers) decreased significantly (P for trend ice cream/sherbet/frozen yogurt increased significantly (P for trend<0·04). Similar trends were observed when the analyses were stratified by sex and BMI. The present results suggest favourable trends in dietary carbohydrate consumption, but dietary guidelines for fruits, vegetables and fibre were not met in this cohort.

  11. Potential impact of rising atmospheric CO2 on quality of grains in chickpea (Cicer arietinum L.).

    Science.gov (United States)

    Saha, Saurav; Chakraborty, Debashis; Sehgal, Vinay K; Pal, Madan

    2015-11-15

    Experiments were conducted in open-top chambers to assess the effect of atmospheric CO2 enrichment (E-CO2) on the quality of grains in chickpea (Cicer arietinum L.) crop. Physical attributes of the grains was not affected, but the hydration and swelling capacities of the flour increased. Increase in carbohydrates and reduction in protein made the grains more carbonaceous (higher C:N) under E-CO2. Among other mineral nutrients, K, Ca and Zn concentrations decreased, while P, Mg, Cu, Fe, Mn and B concentrations did not change. The pH, bulk density and cooking time of chickpea flour remained unaffected, although the water absorption capacity of flour increased and oil absorption reduced. Results suggest that E-CO2 could affect the grain quality adversely and nutritional imbalance in grains of chickpea might occur.

  12. Separation and quantification of microalgal carbohydrates.

    Science.gov (United States)

    Templeton, David W; Quinn, Matthew; Van Wychen, Stefanie; Hyman, Deborah; Laurens, Lieve M L

    2012-12-28

    Structural carbohydrates can constitute a large fraction of the dry weight of algal biomass and thus accurate identification and quantification is important for summative mass closure. Two limitations to the accurate characterization of microalgal carbohydrates are the lack of a robust analytical procedure to hydrolyze polymeric carbohydrates to their respective monomers and the subsequent identification and quantification of those monosaccharides. We address the second limitation, chromatographic separation of monosaccharides, here by identifying optimum conditions for the resolution of a synthetic mixture of 13 microalgae-specific monosaccharides, comprised of 8 neutral, 2 amino sugars, 2 uronic acids and 1 alditol (myo-inositol as an internal standard). The synthetic 13-carbohydrate mix showed incomplete resolution across 11 traditional high performance liquid chromatography (HPLC) methods, but showed improved resolution and accurate quantification using anion exchange chromatography (HPAEC) as well as alditol acetate derivatization followed by gas chromatography (for the neutral- and amino-sugars only). We demonstrate the application of monosaccharide quantification using optimized chromatography conditions after sulfuric acid analytical hydrolysis for three model algae strains and compare the quantification and complexity of monosaccharides in analytical hydrolysates relative to a typical terrestrial feedstock, sugarcane bagasse.

  13. Genetics of carbohydrate accumulation in onion

    Science.gov (United States)

    Fructans are soluble carbohydrates composed of fructose chains attached to a basal sucrose molecule and act both as health-enhancing pro- and pre-biotics. In onion, higher fructan concentrations are correlated with greater soluble solids content, dry weights, and pungency. We analyzed dry weights ...

  14. General Properties, Occurrence, and Preparation of Carbohydrates

    Science.gov (United States)

    Robyt, John F.

    D-Glucose and its derivatives and analogues, N-acetyl-D-glucosamine, N-acetyl-D-muramic acid, D-glucopyranosyl uronic acid, and D-glucitol represent 99.9% of the carbohydrates on the earth. D-Glucose is found in the free state in human blood and in the combined state in disaccharides, sucrose, lactose, and α,α-trehalose, in cyclic dextrins, and in polysaccharides, starch, glycogen, cellulose, dextrans; N-acetyl-D-glucosamine and an analogue N-acetyl-D-muramic acid are found in bacterial cell wall polysaccharide, murein, along with teichoic acids made up of poly-glycerol or -ribitol phosphodiesters. Other carbohydrates, D-mannose, D-mannuronic acid, D-galactose, N-acetyl-D-galactosamine, D-galacturonic acid, D-iduronic acid, L-guluronic acid, L-rhamnose, L-fucose, D-xylose, and N-acetyl-D-neuraminic acid are found in glycoproteins, hemicelluloses, glycosaminoglycans, and polysaccharides of plant exudates, bacterial capsules, alginates, and heparin. D-Ribofuranose-5-phosphate is found in many coenzymes and is the backbone of RNAs (ribonucleic acid), and 2-deoxy-D-ribofuranose-5-phosphate is the backbone of DNA (deoxyribonucleic acid). D-Fructofuranose is found in sucrose, inulin, and levan. The general properties and occurrence of these carbohydrates and general methods of isolation and preparation of carbohydrates are presented.

  15. Selective carbohydrate utilization by lactobacilli and bifidobacteria

    NARCIS (Netherlands)

    Watson, D.; O'Connell Motherway, M.; Schoterman, M.H.C.; Neerven, van R.J.J.; Nauta, A.; Sinderen, van D.

    2013-01-01

    Aim - To evaluate the ability of specific carbohydrates, including commercially available products, to support the growth of representatives of two well-known groups of gut commensals, namely lactobacilli and bifidobacteria. Methods and Results - Sixty-eight bacterial strains, representing 29 human-

  16. Biofortification and localization of zinc in wheat grain.

    Science.gov (United States)

    Cakmak, I; Kalayci, M; Kaya, Y; Torun, A A; Aydin, N; Wang, Y; Arisoy, Z; Erdem, H; Yazici, A; Gokmen, O; Ozturk, L; Horst, W J

    2010-08-25

    the endosperm part that is the predominant grain fraction consumed in many countries. Providing a large pool of Zn in vegetative tissues during the grain filling (e.g., via foliar Zn spray) is an important practice to increase grain Zn and contribute to human nutrition.

  17. Effects of grain size and porosity on strength of Li2TiO3 tritium breeding pebbles and its grain growth behavior

    Science.gov (United States)

    Xiang, Maoqiao; Zhang, Yingchun; Zhang, Yun; Wang, Chaofu; Liu, Wei; Yu, Yonghong

    2016-12-01

    Tons of Li2TiO3 tritium breeding pebbles will be filled in the blanket for obtaining tritium fuel. In this work, isothermal sintering was carried out to study the grain growth behavior of the Li2TiO3 pebbles fabricated by agarose method. The grain growth exponent (n) and the activation energy (Q) calculated by the phenomenological kinetic equation were 2 and 435.65 kJ/mol, respectively. The grain growth was controlled by vapor transport (p = 2S/r). In addition, effects of porosity and grain-size on the strength of Li2TiO3 pebbles were investigated. The strength was affected by the grain size and the porosity of Li2TiO3 pebbles, and high strength (about 72 MPa) depended partly on achieving the optimum balance between the porosity (about 10%) and grain size (about 2 μm).

  18. Filling of carbon nanotubes and nanofibres

    Directory of Open Access Journals (Sweden)

    Reece D. Gately

    2015-02-01

    Full Text Available The reliable production of carbon nanotubes and nanofibres is a relatively new development, and due to their unique structure, there has been much interest in filling their hollow interiors. In this review, we provide an overview of the most common approaches for filling these carbon nanostructures. We highlight that filled carbon nanostructures are an emerging material for biomedical applications.

  19. Cell surface carbohydrates as prognostic markers in human carcinomas

    DEFF Research Database (Denmark)

    Dabelsteen, Erik

    1996-01-01

    Tumour development is usually associated with changes in cell surface carbohydrates. These are often divided into changes related to terminal carbohydrate structures, which include incomplete synthesis and modification of normally existing carbohydrates, and changes in the carbohydrate core...... structure. The latter includes chain elongation of both glycolipids and proteins, increased branching of carbohydrates in N-linked glycoproteins, and blocked synthesis of carbohydrates in O-linked mucin-like glycoproteins. In mature organisms, expression of distinct carbohydrates is restricted to specific...... cell types; within a given tissue, variation in expression may be related to cell maturation. Tumour-associated carbohydrate structures often reflect a certain stage of cellular development; most of these moieties are structures normally found in other adult or embryonic tissues. There is no unique...

  20. A cereal-based evening meal rich in indigestible carbohydrates increases plasma butyrate the next morning

    DEFF Research Database (Denmark)

    Nilsson, Anne C; Östman, Elin M; Knudsen, Knud Erik Bach;

    2010-01-01

    Epidemiological studies have shown an inverse relation between a whole grain consumption and risk of type-2 diabetes and cardiovascular disease. One tentative mechanism relates to colonic metabolism of indigestible carbohydrates. In a previous study, we reported a positive relation between colonic...... fermentation and improved glucose tolerance. This work can be seen as an extension of that study, focusing on the tentative role of specific colonic metabolites, i.e. SCFA. Plasma concentrations of acetate, propionate, and butyrate were determined in the morning in healthy participants (5 women and 10 men...... concentrations the following morning compared with an evening meal with white wheat bread (P fermentation and generation of SCFA, where in particular...

  1. Whole Grains and Fiber

    Science.gov (United States)

    ... whole grains. Does not contain partially hydrogenated oils. Dietary Fiber Dietary fiber is the term for several materials that make ... water. When eaten regularly as part of a diet low in saturated fat and trans fat soluble fiber has been associated with increased diet quality and ...

  2. Formation on grain surfaces

    NARCIS (Netherlands)

    Cazaux, S; Tielens, AGGM

    2004-01-01

    The most abundant interstellar molecule, H-2, is generally thought to form by recombination of H atoms on grain surfaces. On surfaces, hydrogen atoms can be physisorbed and chemisorbed and their mobility can be governed by quantum mechanical tunneling or thermal hopping. We have developed a model fo

  3. Exercise and Regulation of Carbohydrate Metabolism.

    Science.gov (United States)

    Mul, Joram D; Stanford, Kristin I; Hirshman, Michael F; Goodyear, Laurie J

    2015-01-01

    Carbohydrates are the preferred substrate for contracting skeletal muscles during high-intensity exercise and are also readily utilized during moderate intensity exercise. This use of carbohydrates during physical activity likely played an important role during the survival of early Homo sapiens, and genes and traits regulating physical activity, carbohydrate metabolism, and energy storage have undoubtedly been selected throughout evolution. In contrast to the life of early H. sapiens, modern lifestyles are predominantly sedentary. As a result, intake of excessive amounts of carbohydrates due to the easy and continuous accessibility to modern high-energy food and drinks has not only become unnecessary but also led to metabolic diseases in the face of physical inactivity. A resulting metabolic disease is type 2 diabetes, a complex endocrine disorder characterized by abnormally high concentrations of circulating glucose. This disease now affects millions of people worldwide. Exercise has beneficial effects to help control impaired glucose homeostasis with metabolic disease, and is a well-established tool to prevent and combat type 2 diabetes. This chapter focuses on the effects of exercise on carbohydrate metabolism in skeletal muscle and systemic glucose homeostasis. We will also focus on the molecular mechanisms that mediate the effects of exercise to increase glucose uptake in skeletal muscle. It is now well established that there are different proximal signaling pathways that mediate the effects of exercise and insulin on glucose uptake, and these distinct mechanisms are consistent with the ability of exercise to increase glucose uptake in the face of insulin resistance in people with type 2 diabetes. Ongoing research in this area is aimed at defining the precise mechanism by which exercise increases glucose uptake and insulin sensitivity and the types of exercise necessary for these important health benefits.

  4. Possible application of brewer's spent grain in biotechnology

    OpenAIRE

    Pejin Jelena D.; Radosavljević Miloš S.; Grujić Olgica S.; Mojović Ljiljana V.; Kocić-Tanackov Sunčica D.; Nikolić Svetlana B.; Đukić-Vuković Aleksandra J.

    2013-01-01

    Brewer’s spent grain is the major by-product in beer production. It is produced in large quantities (20 kg per 100 liters of produced beer) throughout the year at a low cost or no cost, and due to its high protein and carbohydrates content it can be used as a raw material in biotechnology. Biotechnological processes based on renewable agro-industrial by-products have ecological (zero CO2 emission, eco-friendly by-products) and economical (cheap raw materials and reduction of storage cos...

  5. Carbohydrate Mimetic Peptides Augment Carbohydrate-Reactive Immune Responses in the Absence of Immune Pathology

    OpenAIRE

    Thomas Kieber-Emmons; Anastas Pashov; Behjatolah Monzavi-Karbassi; Fariba Jousheghany; Cecile Artaud; Leah Hennings

    2011-01-01

    Among the most challenging of clinical targets for cancer immunotherapy are Tumor Associated Carbohydrate Antigens (TACAs). To augment immune responses to TACA we are developing carbohydrate mimetic peptides (CMPs) that are sufficiently potent to activate broad-spectrum anti-tumor reactivity. However, the activation of immune responses against terminal mono- and disaccharide constituents of TACA raises concerns regarding the balance between “tumor destruction” and “tissue damage”, as mono- an...

  6. Evaluating the Effect of Dental Filling Material and Filling Depth on the Strength and Deformation of Filled Teeth

    Directory of Open Access Journals (Sweden)

    Seifollah Gholampour

    2016-10-01

    Full Text Available ackground and aim: It is important to evaluate the effect of the type of filling material on deformation and strength of tooth after filling and also the effect of filling depth on quality of restoration of a decayed tooth. Material and Methods: The Orthopantomogram (OPG of the first and second molars of a 28-year-old man was made and the teeth were 3D modeled. The stress-deformation analysis was then performed on the models in the three states of normal tooth, tooth filled with amalgam and tooth filled with composite using finite element method under a distributed load of 400N equivalent to chewing force. Two values (1/2 and 1/3 of the tooth height were considered for filling depth in the analyses. Results: The results showed that the normal first molar was exposed to a 7.2% greater risk of dental injuries compared to the normal second molar and also a greater stress is created in it when it is filled with composite. The first molar filled with a composite material is 13.7% weaker than the normal tooth while it is almost as strong as a normal tooth when it is filled with amalgam. The effect of the type of filling material on the strength and deformation of the second molar was trivial. Conclusion: Amalgam is a more proper dental filling material for the first molar although a 16.7% change in drilling depth is needed for tooth preparation. Dental filling material and filling depth have a small effect on the strength and deformation of filled second molars.

  7. The effect of stereochemistry on carbohydrate hydration in aqueous solutions

    NARCIS (Netherlands)

    Galema, Saskia Alexandra

    1992-01-01

    Although-carbohydrates are widely used, not much is known about the stereochemical aspects of hydration of carbohydrates. For D-aldohexoses, for example, there are eight different stereoisomers. Just how the hydroxy topology of a carbohydrate molecule influences the hydration behaviour in water is r

  8. Grain Boundary Segregation in Metals

    CERN Document Server

    Lejcek, Pavel

    2010-01-01

    Grain boundaries are important structural components of polycrystalline materials used in the vast majority of technical applications. Because grain boundaries form a continuous network throughout such materials, their properties may limit their practical use. One of the serious phenomena which evoke these limitations is the grain boundary segregation of impurities. It results in the loss of grain boundary cohesion and consequently, in brittle fracture of the materials. The current book deals with fundamentals of grain boundary segregation in metallic materials and its relationship to the grain boundary structure, classification and other materials properties.

  9. Why Is It Important to Eat Grains, Especially Whole Grains?

    Science.gov (United States)

    ... Nutrients and health benefits Print Share Why is it important to eat grains, especially whole grains? Eating ... diabetes. Fiber is important for proper bowel function. It helps reduce constipation and diverticulosis. Fiber-containing foods ...

  10. Electron paramagnetic resonance (EPR) spectroscopy characterization of wheat grains from plants of different water stress tolerance.

    Science.gov (United States)

    Łabanowska, Maria; Filek, Maria; Kurdziel, Magdalena; Bednarska, Elżbieta; Dłubacz, Aleksandra; Hartikainen, Helina

    2012-09-01

    Grains of five genotypes of wheat (four Polish and one Finnish), differing in their tolerance to drought stress were chosen for this investigation. Electron paramagnetic resonance spectroscopy allowed observation of transition metal ions (Mn, Fe, Cu) and different types of stable radicals, including semiquinone centers, present in seed coats, as well as several types of carbohydrate radicals found mainly in the inner parts of grains. The content of paramagnetic metal centers was higher in sensitive genotypes (Radunia, Raweta) than in tolerant ones (Parabola, Nawra), whereas the Finnish genotype (Manu) exhibited intermediate amounts. Similarly, the concentrations of both types of radicals, carbohydrates and semiquinone were significantly higher in the grains originating from more sensitive wheat genotypes. The nature of carbohydrate radicals and their concentrations were confronted with the kinds and amounts of sugars found by the biochemical analyses and microscopy observations. It is suggested that some long lived radicals (semiquinone and starch radicals) occurring in grains could be indicators of stress resistance of wheat plants.

  11. Grain rotation mediated by grain boundary dislocations in nanocrystalline platinum

    Science.gov (United States)

    Wang, Lihua; Teng, Jiao; Liu, Pan; Hirata, Akihiko; Ma, En; Zhang, Ze; Chen, Mingwei; Han, Xiaodong

    2014-01-01

    Grain rotation is a well-known phenomenon during high (homologous) temperature deformation and recrystallization of polycrystalline materials. In recent years, grain rotation has also been proposed as a plasticity mechanism at low temperatures (for example, room temperature for metals), especially for nanocrystalline grains with diameter d less than ~15 nm. Here, in tensile-loaded Pt thin films under a high-resolution transmission electron microscope, we show that the plasticity mechanism transitions from cross-grain dislocation glide in larger grains (d>6 nm) to a mode of coordinated rotation of multiple grains for grains with d<6 nm. The mechanism underlying the grain rotation is dislocation climb at the grain boundary, rather than grain boundary sliding or diffusional creep. Our atomic-scale images demonstrate directly that the evolution of the misorientation angle between neighbouring grains can be quantitatively accounted for by the change of the Frank–Bilby dislocation content in the grain boundary. PMID:25030380

  12. Possible application of brewer’s spent grain in biotechnology

    Directory of Open Access Journals (Sweden)

    Pejin Jelena D.

    2013-01-01

    Full Text Available Brewer’s spent grain is the major by-product in beer production. It is produced in large quantities (20 kg per 100 liters of produced beer throughout the year at a low cost or no cost, and due to its high protein and carbohydrates content it can be used as a raw material in biotechnology. Biotechnological processes based on renewable agro-industrial by-products have ecological (zero CO2 emission, eco-friendly by-products and economical (cheap raw materials and reduction of storage costs advantages. The use of brewer’s spent grain is still limited, being basically used as animal feed. Researchers are trying to improve the application of brewer’s spent grain by finding alternative uses apart from the current general use as an animal feed. Its possible applications are in human nutrition, as a raw material in biotechnology, energy production, charcoal production, paper manufacture, as a brick component, and adsorbent. In biotechnology brewer’s spent grain could be used as a substrate for cultivation of microorganisms and enzyme production, additive of yeast carrier in beer fermentation, raw material in production of lactic acid, bioethanol, biogas, phenolic acids, xylitol, and pullulan. Some possible applications for brewer’s spent grain are described in this article including pre-treatment conditions (different procedures for polysaccharides, hemicelluloses, and cellulose hydrolysis, working microorganisms, fermentation parameters and obtained yields. The chemical composition of brewer’s spent grain varies according to barley variety, harvesting time, malting and mashing conditions, and a quality and type of unmalted raw material used in beer production. Brewer’s spent grain is lignocellulosic material rich in protein and fibre, which account for approximately 20 and 70% of its composition, respectively.

  13. Organotin-catalyzed highly regioselective thiocarbonylation of nonprotected carbohydrates and synthesis of deoxy carbohydrates in a minimum number of steps.

    Science.gov (United States)

    Muramatsu, Wataru; Tanigawa, Satoko; Takemoto, Yuki; Yoshimatsu, Hirofumi; Onomura, Osamu

    2012-04-16

    Nonprotected carbohydrates: The catalytic regioselective thiocarbonylation of carbohydrates by using organotin dichloride under mild conditions was demonstrated. The reaction afforded various deoxy saccharides in high yields and excellent regioselectivity in a minimum number of steps. The regioselectivity of the thiocarbonylation is attributed to the intrinsic character of the carbohydrates based on the stereorelationship of their hydroxy groups (see scheme).

  14. Predictive coarse-graining

    Science.gov (United States)

    Schöberl, Markus; Zabaras, Nicholas; Koutsourelakis, Phaedon-Stelios

    2017-03-01

    We propose a data-driven, coarse-graining formulation in the context of equilibrium statistical mechanics. In contrast to existing techniques which are based on a fine-to-coarse map, we adopt the opposite strategy by prescribing a probabilistic coarse-to-fine map. This corresponds to a directed probabilistic model where the coarse variables play the role of latent generators of the fine scale (all-atom) data. From an information-theoretic perspective, the framework proposed provides an improvement upon the relative entropy method [1] and is capable of quantifying the uncertainty due to the information loss that unavoidably takes place during the coarse-graining process. Furthermore, it can be readily extended to a fully Bayesian model where various sources of uncertainties are reflected in the posterior of the model parameters. The latter can be used to produce not only point estimates of fine-scale reconstructions or macroscopic observables, but more importantly, predictive posterior distributions on these quantities. Predictive posterior distributions reflect the confidence of the model as a function of the amount of data and the level of coarse-graining. The issues of model complexity and model selection are seamlessly addressed by employing a hierarchical prior that favors the discovery of sparse solutions, revealing the most prominent features in the coarse-grained model. A flexible and parallelizable Monte Carlo - Expectation-Maximization (MC-EM) scheme is proposed for carrying out inference and learning tasks. A comparative assessment of the proposed methodology is presented for a lattice spin system and the SPC/E water model.

  15. Grain alcohol study: summary

    Energy Technology Data Exchange (ETDEWEB)

    The study has concentrated upon a detailed examination of all considerations involved in the production, use, and marketing of ethyl alcohol (Ethanol) as produced from the fermentation of agricultural grains. Each parameter was examined in the light of current energy markets and trends; new sources and technological, and processes for fermentation, the capability of the agricultural industry to support fermentaton demand; the optimization of value of agricultureal crops; and the efficiencies of combining related industries. Anhydrous (200 proof) ethanol makes an excellent blending component for all present automotive fuels and an excellent octane additive for unleaded fuels in proportions up to 35% without requiring modifications to current engines. There is no difference between ethanol produced by fermentation and ethanol produced synthetically from petroleum. The decision to produce ethanol one way or the other is purely economic. The agricultural industry can support a major expansion in the fermentation industry. The residue (distillers grains) from the fermentation of corn for ethanol is an excellent and economical feed for livestock and poultry. A reliable supply of distillers grains can assist in making the large beef feedlot operations more economically viable. The source materials, fuels, products and by-products of an ethanol plant, beef feedlot, gas biodigester plant, municipal waste recovery plant and a steam generated electrical plant are interrelated and mutually beneficial for energy efficiencies and economic gains when co-located. The study concludes that the establishment of such agricultural-environment industrial energy complexes, would provide a broad range of significant benefits to Indiana.

  16. Grain alcohol study: summary

    Energy Technology Data Exchange (ETDEWEB)

    The study has concentrated upon a detailed examination of all considerations involved in the production, use, and marketing of ethyl alcohol (ethanol) as produced from the fermentation of agricultural grains. Each parameter was examined in the light of current energy markets and trends; new sources and technological, and processes for fermentation, the capability of the agricultural industry to support fermentation demand; the optimizaton of value of agricultural crops; and the efficiencies of combining related industries. Ahydrous (200 proof) ethanol makes an excellent blending component for all present automotive fuels and an excellent octane additive for unleaded fuels in proportions up to 35% without requiring modifications to current engines. There is no difference between ethanol produced by fermentation and ethanol produced synthetically from petroleum. The decision to produce ethanol one way or the other is purely economic. The agricultural industry can support a major expansion in the fermentation industry. The residue (distillers grains) from the fermentation of corn for ethanol is an excellent and economical feed for livestock and poultry. A reliable supply of distillers grain can assist in making the large beef feedlot operations more economically viable. The source materials, fuels, products and by-products of an ethanol plant, beef feedlot, gas biodigester plant, municipal waste recovery plant and a steam generated electrical plant are interrelated and mutually beneficial for energy efficiencies and economic gains when co-located. The study concludes that the establishment of such agricultural- environment industrial energy complexes, would provide a broad range of significant benefits to Indiana.

  17. Daily training with high carbohydrate availability increases exogenous carbohydrate oxidation during endurance cycling.

    Science.gov (United States)

    Cox, Gregory R; Clark, Sally A; Cox, Amanda J; Halson, Shona L; Hargreaves, Mark; Hawley, John A; Jeacocke, Nikki; Snow, Rodney J; Yeo, Wee Kian; Burke, Louise M

    2010-07-01

    We determined the effects of varying daily carbohydrate intake by providing or withholding carbohydrate during daily training on endurance performance, whole body rates of substrate oxidation, and selected mitochondrial enzymes. Sixteen endurance-trained cyclists or triathletes were pair matched and randomly allocated to either a high-carbohydrate group (High group; n = 8) or an energy-matched low-carbohydrate group (Low group; n = 8) for 28 days. Immediately before study commencement and during the final 5 days, subjects undertook a 5-day test block in which they completed an exercise trial consisting of a 100 min of steady-state cycling (100SS) followed by a 7-kJ/kg time trial on two occasions separated by 72 h. In a counterbalanced design, subjects consumed either water (water trial) or a 10% glucose solution (glucose trial) throughout the exercise trial. A muscle biopsy was taken from the vastus lateralis muscle on day 1 of the first test block, and rates of substrate oxidation were determined throughout 100SS. Training induced a marked increase in maximal citrate synthase activity after the intervention in the High group (27 vs. 34 micromol x g(-1) x min(-1), P < 0.001). Tracer-derived estimates of exogenous glucose oxidation during 100SS in the glucose trial increased from 54.6 to 63.6 g (P < 0.01) in the High group with no change in the Low group. Cycling performance improved by approximately 6% after training. We conclude that altering total daily carbohydrate intake by providing or withholding carbohydrate during daily training in trained athletes results in differences in selected metabolic adaptations to exercise, including the oxidation of exogenous carbohydrate. However, these metabolic changes do not alter the training-induced magnitude of increase in exercise performance.

  18. Effects of Four Pesticides on Grain Growth Parameters of Rice

    Institute of Scientific and Technical Information of China (English)

    WU Jin-cai; DONG Bo; LI Dong-hu; QIU Hui-min; YANG Guo-qing

    2004-01-01

    Effect of four commonly used pesticides, triadlmefon, jingganmycin, triazoplos and imidacloprid, on grain growth parameters was examined using a growth equation in the present paper. Two hundreds of spikes, not damaged by pests were simultaneously marked per plot during the heading stage of rice. Rice plants were sprayed at 1 d after marking.Thereafter, 20 spikes were sampled at 4 d intervals and dried in an oven. Then, 10 superior and inferior grains were picked from each spike, and brown rice was weighed after shelling, respectively. First, second and third order derivates were deduced from the following grain growth formula:W=K/1+ea-bt , where W was the weight of 100 grains brown rice at time t; K was maximum of grain growth; a and b were parameters of the formula. The parameters were calculated as following:R0=Kbea/ (1 + ea)2 ,△ t=t2-t1,GT=bk/4(t2-t1)=Vmax(t2-t1),Vmax=bk/4Where R0, △t, GT and Vmax were initial growth power, active growth stage, accumulative weight of dried content during △ t and maximum growth rate, respectively. The result showed that GT and K of superior grain following 22.5 and 45 ga.i. ha-1 of imidacloprid sprays were significantly reduced, and △t was significantly decreased for 112.5 and 225ga.i. ha-1 jingganmycin treatments. In addition, the high dose of imidacloprid significantly reduced the weight of 1 000 rice grains by 9.77%. However, there was no significant difference for the weight of 1 000 grains between the high dose of jingganmycin and thecontrol, indicating that effective duration of jingganmycin on grain filling was shorterthan that of imidacloprid.

  19. Functionalized carbon nanomaterials derived from carbohydrates.

    Science.gov (United States)

    Jagadeesan, Dinesh; Eswaramoorthy, Muthusamy

    2010-02-01

    A tremendous growth in the field of carbon nanomaterials has led to the emergence of carbon nanotubes, fullerenes, mesoporous carbon and more recently graphene. Some of these materials have found applications in electronics, sensors, catalysis, drug delivery, composites, and so forth. The high temperatures and hydrocarbon precursors involved in their synthesis usually yield highly inert graphitic surfaces. As some of the applications require functionalization of their inert graphitic surface with groups like -COOH, -OH, and -NH(2), treatment of these materials in oxidizing agents and concentrated acids become inevitable. More recent works have involved using precursors like carbohydrates to produce carbon nanostructures rich in functional groups in a single-step under hydrothermal conditions. These carbon nanostructures have already found many applications in composites, drug delivery, materials synthesis, and Li ion batteries. The review aims to highlight some of the recent developments in the application of carbohydrate derived carbon nanostructures and also provide an outlook of their future prospects.

  20. Carbohydrate plasma expanders for passive tumor targeting

    DEFF Research Database (Denmark)

    Hoffmann, Stefan; Caysa, Henrike; Kuntsche, Judith

    2013-01-01

    The objective of this study was to investigate the suitability of carbohydrate plasma volume expanders as a novel polymer platform for tumor targeting. Many synthetic polymers have already been synthesized for targeted tumor therapy, but potential advantages of these carbohydrates include...... inexpensive synthesis, constant availability, a good safety profile, biodegradability and the long clinical use as plasma expanders. Three polymers have been tested for cytotoxicity and cytokine activation in cell cultures and conjugated with a near-infrared fluorescent dye: hydroxyethyl starches (HES 200 k......Da and HES 450 kDa) and dextran (DEX 500 kDa). Particle size and molecular weight distribution were determined by asymmetric flow field-flow fractionation (AF4). The biodistribution was investigated non-invasively in nude mice using multispectral optical imaging. The most promising polymer conjugate...

  1. Small-Molecule Carbohydrate-Based Immunostimulants.

    Science.gov (United States)

    Marzabadi, Cecilia H; Franck, Richard W

    2017-02-03

    In this review, we discuss small-molecule, carbohydrate-based immunostimulants that target Toll-like receptor 4 (TLR-4) and cluster of differentiation 1D (CD1d) receptors. The design and use of these molecules in immunotherapy as well as results from their use in clinical trials are described. How these molecules work and their utilization as vaccine adjuvants are also discussed. Future applications and extensions for the use of these analogues as therapeutic agents will be outlined.

  2. Crystallisation and crystal forms of carbohydrate derivatives

    Science.gov (United States)

    Lennon, Lorna

    This thesis is focused on the synthesis and solid state analysis of carbohydrate derivatives, including many novel compounds. Although the synthetic chemistry surrounding carbohydrates is well established in the literature, the crystal chemistry of carbohydrates is less well studied. Therefore this research aims to improve understanding of the solid state properties of carbohydrate derivatives through gaining more information on their supramolecular bonding. Chapter One focuses on an introduction to the solid state of organic compounds, with a background to crystallisation, including issues that can arise during crystal growth. Chapter Two is based on glucopyranuronate derivatives which are understudied in terms of their solid state forms. This chapter reports on the formation of novel glucuronamides and utilising the functionality of the amide bond for crystallisation. TEMPO oxidation was completed to form glucopyranuronates by oxidation of the primary alcohol groups of glucosides to the carboxylic acid derivatives, to increase functionality for enhanced crystal growth. Chapter Three reports on the synthesis of glucopyranoside derivatives by O-glycosylation reactions and displays crystal structures, including a number of previously unsolved acetate protected and deprotected crystal structures. More complex glycoside derivatives were also researched in an aim to study the resultant supramolecular motifs. Chapter Four contains the synthesis of aryl cellobioside derivatives including the novel crystal structures that were solved for the acetate protected and deprotected compounds. Research was carried out to determine if 1-deoxycellodextrins could act as putative isostructures for cellulose. Our research displays the presence of isostructural references with 1-deoxycellotriose shown to be similar to cellulose III11, 1-deoxycellotetraose correlates with cellulose IV11 and 1-deoxycellopentose shows isostructurality similar to that of cellulose II. Chapter Five contains

  3. Direct synthesis of methyl phosphoramidates in carbohydrates.

    Science.gov (United States)

    Dhurandhare, Vijay M; Mishra, Girija Prasad; Lam, Sarah; Wang, Cheng-Chung

    2015-09-28

    A direct installation of a methyl phosphoramidate group by using methyl benzylphosphoramidochloridate into carbohydrates and amino acid is described. This one-step synthesis is efficient for both primary and secondary alcohols and exhibited excellent regioselectivity and functional group compatibility. Formation of a single diastereomer is observed in certain cases. The N-benzyl protecting group on methyl phosphoramidates is easily removed under mild conditions.

  4. Evaluating the Effect of Dental Filling Material and Filling Depth on the Strength and Deformation of Filled Teeth

    OpenAIRE

    Seifollah Gholampour; Ghazale Zoorazma; Ehsan Shakouri

    2016-01-01

    ackground and aim: It is important to evaluate the effect of the type of filling material on deformation and strength of tooth after filling and also the effect of filling depth on quality of restoration of a decayed tooth. Material and Methods: The Orthopantomogram (OPG) of the first and second molars of a 28-year-old man was made and the teeth were 3D modeled. The stress-deformation analysis was then performed on the models in the three states of normal tooth, tooth filled with amalgam and ...

  5. Hearing Loss, Dizziness, and Carbohydrate Metabolism

    Directory of Open Access Journals (Sweden)

    Albernaz, Pedro L. Mangabeira

    2015-07-01

    Full Text Available Introduction Metabolic activity of the inner ear is very intense, and makes it sensitive to changes in the body homeostasis. This study involves a group of patients with inner ear disorders related to carbohydrate metabolism disturbances, including hearing loss, tinnitus, dizziness, and episodes of vertigo. Objectives To describe the symptoms of metabolic inner ear disorders and the examinations required to establish diagnoses. These symptoms are often the first to allow for an early diagnosis of metabolic disorders and diabetes. Methods Retrospective study of 376 patients with inner ear symptoms suggestive of disturbances of carbohydrate metabolism. The authors present patientś clinical symptoms and clinical evaluations, with emphasis on the glucose and insulin essays. Results Authors based their conclusions on otolaryngological findings, diagnostic procedures and treatment principles. They found that auditory and vestibular symptoms usually occur prior to other manifestations of metabolic changes, leading to an early diagnosis of hyperinsulinemia, intestinal sugar malabsorption or diabetes. Previously undiagnosed diabetes mellitus type II was found in 39 patients. Conclusions The identification of carbohydrate metabolism disturbances is important not only to minimize the patients' clinical symptoms, but also to help maintain their general health.

  6. UV-B radiation does not limit carbohydrate level and carbohydrate metabolism in cucumber leaves

    Directory of Open Access Journals (Sweden)

    Magdalena Rybus-Zając

    2014-08-01

    Full Text Available Cucumber is a vegetable exhibiting relatively high sensitivity to environmental stress factors. When it is grown outdoors, from early stages of development there is a real risk of exposure to elevated UV-B radiation. In order to explain the effects of time-dependent UV-B doses on carbohydrate level and metabolism, the photosynthetic activity, accumulation of carbohydrates and activities of carbohydrate-related enzymes were determined in the cucumber leaves. Elevated UV-B radiation led to an increase in the rate of photosynthesis, which was reflected by an increase in SPAD values. Higher photosynthetic activity resulted in an increase in levels of soluble sugars. In view of the above-mentioned results, radiation stress led to a UV-B time-dependent dose increase in the activity of two enzymes decomposing carbohydrate: invertase and glucosidase. Our results suggest that the exposure of cucumber plants to supplemental UV-B doses does not limit the availability of the photoassimilate. Carbohydrates are required to provide not only respiratory energy for protection, maintenance (and repair of plant activity and structure, but also provide biosynthetic carbon skeletons for secondary metabolite synthesis

  7. Dedicated composite fillings − inlays

    Directory of Open Access Journals (Sweden)

    Šaulić Slobodan

    2003-01-01

    Full Text Available Background. The aim of the study was to evaluate the quality and persistance of esthetics of dedicated inlay by clinical methods. Methods. The paper reviews the clinical significance and technique of preparing particular composite inlays before and after the construction of the metallic framework partial denture. On the basis of indications the total of 30 inlays were placed into cavities under relatively dry working conditions. Six, twelve eighteen and twenty-four months after the placement of filling, control check-up was carried out by Ryge criteria. Results. After two years marginal discoloration as well as the change of the colour occured in 3.3% of inlays. There was neither detectable secondary caries, nor the symtoms of pulpal damage. The requirements to be fulfilled concerning the composite materials in order that they can be implemented for this purpose, were also discussed. Conclusion. From the clinical point of view, purpouse inlays from Herculite XRV lab C8B in combination with Opti Bond System and composite cement Porcelite Dual Cure showed high functional and esthetic values in the observational period of two years.

  8. Mechanism and calculation method of rheological settlement of high-filled embankment

    Institute of Scientific and Technical Information of China (English)

    王智超; 罗迎社; 唐松花

    2008-01-01

    The characteristics of high-filled embankment rheological settlement were analyzed;mechanical calculation model of high-filled embankment rheological settlement during constructing and running period was also put forward.Combining the macroscopic and microscopic deformation properties of the engineering soil grain,its constitutive model was set up and its characters were fully revealed,at the same time,its practical calculation formula under the action of dead-weight load was derived,which is feasible by analysis and comparison.

  9. Grain Destruction in Interstellar Shocks

    OpenAIRE

    1995-01-01

    Interstellar shock waves can erode and destroy grains present in the shocked gas, primarily as the result of sputtering and grain-grain collisions. Uncertainties in current estimates of sputtering yields are reviewed. Results are presented for the simple case of sputtering of fast grains being stopped in cold gas. An upper limit is derived for sputtering of refractory grains in C-type MHD shocks: shock speeds $v_s \\gtrsim 50 \\kms$ are required for return of more than 30\\% of the silicate to t...

  10. Study on Nitrogen Distribution in Leaf, Stem and Sheath at Different Layers in Winter Wheat Canopy and Their Influence on Grain Quality

    Institute of Scientific and Technical Information of China (English)

    WANG Zhi-jie; WANG Ji-hua; HUANG Wen-jiang; MA Zhi-hong; ZHAO Ming

    2003-01-01

    Vertical distribution of nitrogen in wheat canopy, nitrogen remobilization and their influence on grain quality of winter wheat were studied. Two winter wheat cultivars, Jingdong8, a common cultivar, and Zhongyou9507, a high quality cultivar, were selected. Leaf nitrogen showed an obvious decreasing trend from the canopy top to the ground surface for all treatments in growth duration. There was no apparent vertical nitrogen gradient in stem and sheath of Zhongyou9507 compared with Jingdong8. Zhongyou9507 had more nitrogen remobilization from leaf, stem and sheath than Jingdong8 from middle grain filling to waxening, especially the nitrogen remobilization amount in stem and sheath, which was higher than that in Jingdong8 during growth duration. Higher vertical nitrogen gradients in Jingdong8 at anthesis had disadvantages on its grain quality. But higher vertical nitrogen gradients between middle and lower layers of Jingdong8 at grain filling stage enhanced its grain quality. Higher vertical nitrogen gradients in upper layer at anthesis and upper layer leaf and middle layer stem and sheath at grain filling stage had advantages on protein accumulation in grain of Zhongyou9507. There were positive correlations between foliar nitrogen remobilization amount and grain quality at later growth stage for the two cultivars. There was a positive correlation between quality of Jingdong8 and stem and sheath nitrogen remobilization amount from anthesis to early grain filling, and that of Zhongyou9507 emerged from anthesis to early grain filling and from middle grain filling to waxening. Contribution of leaf nitrogen to the quality of Jingdong8 was larger than nitrogen from stem and sheath. High protein content of Zhongyou9507 was attributed to the nitrogen condition in its leaf, stem and sheath. Nitrogen in stem and sheath played a more important role on the grain quality of Zhongyou9507 than on that of Jingdong8.

  11. Surfaces that become isotopic after Dehn filling

    CERN Document Server

    Bachman, David; Sedgwick, Eric

    2010-01-01

    We show that after generic filling along a torus boundary component of a 3-manifold, no two closed, 2-sided, essential surfaces become isotopic, and no closed, 2-sided, essential surface becomes inessential. That is, the set of essential surfaces (considered up to isotopy) survives unchanged in all suitably generic Dehn fillings. Furthermore, for all but finitely many non-generic fillings, we show that two essential surfaces can only become isotopic in a constrained way.

  12. Whole grains and human health.

    Science.gov (United States)

    Slavin, Joanne

    2004-06-01

    Epidemiological studies find that whole-grain intake is protective against cancer, CVD, diabetes, and obesity. Despite recommendations to consume three servings of whole grains daily, usual intake in Western countries is only about one serving/d. Whole grains are rich in nutrients and phytochemicals with known health benefits. Whole grains have high concentrations of dietary fibre, resistant starch, and oligosaccharides. Whole grains are rich in antioxidants including trace minerals and phenolic compounds and these compounds have been linked to disease prevention. Other protective compounds in whole grains include phytate, phyto-oestrogens such as lignan, plant stanols and sterols, and vitamins and minerals. Published whole-grain feeding studies report improvements in biomarkers with whole-grain consumption, such as weight loss, blood-lipid improvement, and antioxidant protection. Although it is difficult to separate the protective properties of whole grains from dietary fibre and other components, the disease protection seen from whole grains in prospective epidemiological studies far exceeds the protection from isolated nutrients and phytochemicals in whole grains.

  13. Diurnal O2 and carbohydrate levels in wheat kernels during embryony.

    Science.gov (United States)

    Carman, John G; Bishop, Deborah L

    2004-09-01

    In vitro zygotic and somatic embryogenesis procedures for wheat have been improved by simulating in ovulo nutritional, hormonal and dissolved oxygen (dO2) conditions. However, diurnal fluctuations in these conditions during early embryony are not well characterized. In this study, dO2 and water-soluble carbohydrate Levels in wheat kernels were determined after 8 h of light and 8 h of dark at approximately 6, 12 and 18 day post anthesis (DPA). Clark style O2 microelectrodes, having a tip diameter of approximately 115 microm, were inserted into intact kernels immediately distil to the developing embryo, and dO2 levels were recorded at 50 microm intervals into the center of kernels. High-performance anion exchange chromatography with pulsed amperometric detection was used to quantify carbohydrate levels in endosperm sap. dO2 levels in the chlorophyllous layer of the pericarp reached 190 mmoLm(-3) during the day, which probably represents, because of photosynthesis, a supersaturated O2 condition relative to the external environment (21% O2). At the embryo surface, dO2 levels at 6 DPA ranged from 135 to 170 mmolm(-3). At 12 and 18 DPA, dO2 levels at the embryo axis ranged from 100 to 150mmolm(-3). At all three stages, dO2 levels in the center of the endosperm were below 13 mmolm(-3). Extreme fluctuations in carbohydrate levels were observed diurnally during rapid seed fill (12DPA). Levels of sucrose and short-chain fructans were much higher during the day than during the night. In contrast, fructose, glucose, and myo-inositol levels were much higher during the night than during the day. By 18DPA (hard dough stage), carbohydrate levels tended to be similar during the day and night. These dynamic fluctuations may assist in regulating embryony in ovulo, and their simulation might improve the development of somatic and zygotic embryos in vitro.

  14. Alternative grains in nutrition

    Directory of Open Access Journals (Sweden)

    Jevcsák Sz.

    2016-12-01

    Full Text Available Many people suffer from gluten sensitivity or gluten intolerance. They have to avoid or limit their gluten intake. Sorghum and millet are gluten-free cereals, wherefore persons with gluten sensitivity or gluten intolerance could consume them. Moreover, they have a lot of positive effects due to their phenolic compounds as phenol acid or flavonoid. Antioxidant activity in sorghum is especially high in comparison with other cereals. Our aim was to compare literature data about the chemical compositions of sorghum and millet with other grains.

  15. Carbohydrate-protein interactions and their biosensing applications.

    Science.gov (United States)

    Zeng, Xiangqun; Andrade, Cesar A S; Oliveira, Maria D L; Sun, Xue-Long

    2012-04-01

    Carbohydrate recognition is clearly present throughout nature, playing a major role in the initial attachment of one biological entity to another. The important question is whether these prevalent interactions could provide a real suitable alternative to the use of antibodies or nucleic acid for detection and identification. Currently, examples of carbohydrates being employed in biological detection systems are limited. The challenges of using carbohydrate recognition for detection mainly come from the weak affinity of carbohydrate-protein interactions, the lack of versatile carbohydrate scaffolds with well-defined structures, and the less developed high-information-content, real-time, and label-free assay technology. In this review, we focus on discussing the characteristics of carbohydrate-protein interactions in nature and the methods for carbohydrate immobilization based on surface coupling chemistry in terms of their general applicability for developing carbohydrate- and lectin-based label-free sensors. Furthermore, examples of innovative design of multivalent carbohydrate-protein interactions for sensor applications are given. We limit our review to show the feasibility of carbohydrate and lectin as recognition elements for label-free sensor development in several representative cases to formulate a flexible platform for their use as recognition elements for real-world biosensor applications.

  16. A step towards personalized sports nutrition: carbohydrate intake during exercise.

    Science.gov (United States)

    Jeukendrup, Asker

    2014-05-01

    There have been significant changes in the understanding of the role of carbohydrates during endurance exercise in recent years, which allows for more specific and more personalized advice with regard to carbohydrate ingestion during exercise. The new proposed guidelines take into account the duration (and intensity) of exercise and advice is not restricted to the amount of carbohydrate; it also gives direction with respect to the type of carbohydrate. Studies have shown that during exercise lasting approximately 1 h in duration, a mouth rinse or small amounts of carbohydrate can result in a performance benefit. A single carbohydrate source can be oxidized at rates up to approximately 60 g/h and this is the recommendation for exercise that is more prolonged (2-3 h). For ultra-endurance events, the recommendation is higher at approximately 90 g/h. Carbohydrate ingested at such high ingestion rates must be a multiple transportable carbohydrates to allow high oxidation rates and prevent the accumulation of carbohydrate in the intestine. The source of the carbohydrate may be a liquid, semisolid, or solid, and the recommendations may need to be adjusted downward when the absolute exercise intensity is low and thus carbohydrate oxidation rates are also low. Carbohydrate intake advice is independent of body weight as well as training status. Therefore, although these guidelines apply to most athletes, they are highly dependent on the type and duration of activity. These new guidelines may replace the generic existing guidelines for carbohydrate intake during endurance exercise.

  17. The Acute Impact of Ingestion of Sourdough and Whole-Grain Breads on Blood Glucose, Insulin, and Incretins in Overweight and Obese Men

    Directory of Open Access Journals (Sweden)

    Anita Mofidi

    2012-01-01

    Full Text Available Consumption of whole-grain and sourdough breads is associated with improved glucose homeostasis. We examined the impact of commercial breads on biomarkers of glucose homeostasis in subjects at risk for glucose intolerance. In a randomized, crossover study, overweight or obese males ingested 11-grain, sprouted-grain, 12-grain, sourdough, or white bread on different occasions, matched for available carbohydrate (50 g in part 1 (n=12 and bread mass (107 g in part 2 (n=11, and blood glucose, insulin and glucose-dependent insulinotropic polypeptide (GIP and glucagon-like peptide-1 (GLP-1 were determined for 3 h. In part 1, glucose response for sprouted-grain was lower than 11-grain, sourdough, and white breads. Insulin area under the curve (AUC for sourdough and white was lower than 11-grain and sprouted-grain breads. GLP-1 response to sourdough was lower than all breads. In part 2, glucose and insulin AUC for sourdough was greater than 11-grain, sprouted-grain, and 12-grain breads. Sprouted-grain bread improved glycemia by lowering glucose response and increasing GLP-1 response. In overweight and obese men, the glycemic response to sprouted grain bread was reduced in both parts 1 and 2 while the other whole-grain test breads did not improve metabolic responses in the acute postprandial state.

  18. The acute impact of ingestion of sourdough and whole-grain breads on blood glucose, insulin, and incretins in overweight and obese men.

    Science.gov (United States)

    Mofidi, Anita; Ferraro, Zachary M; Stewart, Katherine A; Tulk, Hilary M F; Robinson, Lindsay E; Duncan, Alison M; Graham, Terry E

    2012-01-01

    Consumption of whole-grain and sourdough breads is associated with improved glucose homeostasis. We examined the impact of commercial breads on biomarkers of glucose homeostasis in subjects at risk for glucose intolerance. In a randomized, crossover study, overweight or obese males ingested 11-grain, sprouted-grain, 12-grain, sourdough, or white bread on different occasions, matched for available carbohydrate (50 g) in part 1 (n = 12) and bread mass (107 g) in part 2 (n = 11), and blood glucose, insulin and glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) were determined for 3 h. In part 1, glucose response for sprouted-grain was lower than 11-grain, sourdough, and white breads. Insulin area under the curve (AUC) for sourdough and white was lower than 11-grain and sprouted-grain breads. GLP-1 response to sourdough was lower than all breads. In part 2, glucose and insulin AUC for sourdough was greater than 11-grain, sprouted-grain, and 12-grain breads. Sprouted-grain bread improved glycemia by lowering glucose response and increasing GLP-1 response. In overweight and obese men, the glycemic response to sprouted grain bread was reduced in both parts 1 and 2 while the other whole-grain test breads did not improve metabolic responses in the acute postprandial state.

  19. Changes in the nuclear proteome of developing wheat (Triticum aestivum L.) grain

    Science.gov (United States)

    Bonnot, Titouan; Bancel, Emmanuelle; Chambon, Christophe; Boudet, Julie; Branlard, Gérard; Martre, Pierre

    2015-01-01

    Wheat grain end-use value is determined by complex molecular interactions that occur during grain development, including those in the cell nucleus. However, our knowledge of how the nuclear proteome changes during grain development is limited. Here, we analyzed nuclear proteins of developing wheat grains collected during the cellularization, effective grain-filling, and maturation phases of development, respectively. Nuclear proteins were extracted and separated by two-dimensional gel electrophoresis. Image analysis revealed 371 and 299 reproducible spots in gels with first dimension separation along pH 4–7 and pH 6–11 isoelectric gradients, respectively. The relative abundance of 464 (67%) protein spots changed during grain development. Abundance profiles of these proteins clustered in six groups associated with the major phases and phase transitions of grain development. Using nano liquid chromatography-tandem mass spectrometry to analyse 387 variant and non-variant protein spots, 114 different proteins were identified that were classified into 16 functional classes. We noted that some proteins involved in the regulation of transcription, like HMG1/2-like protein and histone deacetylase HDAC2, were most abundant before the phase transition from cellularization to grain-filling, suggesting that major transcriptional changes occur during this key developmental phase. The maturation period was characterized by high relative abundance of proteins involved in ribosome biogenesis. Data are available via ProteomeXchange with identifier PXD002999. PMID:26579155

  20. Molecular Improvement of Grain Weight and Yield in Rice by Using GW6 Gene

    Institute of Scientific and Technical Information of China (English)

    LI Yuan-yuan; HU Jiang; YE Guo-you; GUO Long-biao; TAO Hong-jian; ZHAO Xiang-qian; XU Jie; LI Geng-mi; HU Shi-kai; DONG Guo-jun; SHI Zheng-yuan; WU Li-wen

    2014-01-01

    Molecular design breeding is one of straightforward approaches to break yield barriers in rice. In this study, GW6 gene for grain length and width from Baodali was transferred into an indica recurrent parent 9311 and a japonica variety Zhonghua 11 (ZH11) using marker-assisted backcross (MAB). One and three introgression lines were selected for phenotypic analysis from 9311 and ZH11 genetic backgrounds, respectively. SSL-1, an improved 9311 near isogenic line with GW6 performed 11%, 19% and 6.7%higher of grain length, 1000-grain weight and single plant yield, respectively, as compared with 9311. All the three improved ZH11-GW6 lines, R1, R2 and R3, had more than 30% increase in grain weight and about 7%higher in grain yield. Seed plumpness of R1, R2 and R3 was improved synchronously because the three ZH11-GW6 lines contained GIF1 (Grain Incomplete Filling 1), a dominant grain filling gene. Thus, GW6 has high potential in increasing the yield of inbred lines through MAB, making it an important genetic resource in super hybrid rice breeding. This study provides insights in the utilization of GW6 for large grain and high yield rice breeding via molecular design breeding.

  1. Grain size reduction due to fracturing and subsequent grain-size-sensitive creep in a lower crustal shear zone in the presence of a CO2-bearing fluid

    Science.gov (United States)

    Okudaira, Takamoto; Shigematsu, Norio; Harigane, Yumiko; Yoshida, Kenta

    2017-02-01

    To understand rheological weakening in the lower continental crust, we studied mylonites in the Paleoproterozoic Eidsfjord anorthosite, northern Norway. The zones of anorthositic mylonites range from a few millimeters to several meters thick, and include ultramylonites and protomylonites. They contain syn-kinematic metamorphic minerals, including Cl-bearing amphibole and scapolite. Thermodynamic analysis reveals that syn-deformational hydration reactions occurred at ∼600 °C and ∼700 MPa under CO2-bearing conditions. The protomylonites contain many fragmented plagioclase porphyroclasts. The fractures in porphyroclasts are filled with fine-grained plagioclase, suggesting that fracturing is a common mechanism of grain size reduction. The anorthite contents of fine-grained polygonal matrix plagioclase are different from those of porphyroclastic plagioclase, suggesting that the matrix grains nucleated and grew during syn-kinematic metamorphism. Plagioclase aggregates in the matrices of mylonites do not exhibit a distinct crystallographic preferred orientation, which implies that the dominant deformation mechanism was grain-size-sensitive creep. Consequently, in the lower crustal anorthositic mylonites, grain size reduction occurred via fracturing, rather than through dynamic recrystallization, leading to grain-size-sensitive creep. The syn-kinematic recrystallization of minor phases at plagioclase grain boundaries may suppress the growth of plagioclase and contribute to the development of grain-size-sensitive creep.

  2. Differences in grain ultrastructure, phytochemical and proteomic profiles between the two contrasting grain Cd-accumulation barley genotypes.

    Directory of Open Access Journals (Sweden)

    Hongyan Sun

    Full Text Available To reveal grain physio-chemical and proteomic differences between two barley genotypes, Zhenong8 and W6nk2 of high- and low-grain-Cd-accumulation, grain profiles of ultrastructure, amino acid and proteins were compared. Results showed that W6nk2 possesses significantly lower protein content, with hordein depicting the greatest genotypic difference, compared with Zhenong8, and lower amino acid contents with especially lower proportion of Glu, Tyr, Phe and Pro. Both scanning and transmission electron microscopy observation declared that the size of A-type starch molecule in W6nk2 was considerably larger than that of Zhenong8. Grains of Zhenong8 exhibited more protein-rich deposits around starch granules, with some A-type granules having surface pits. Seventeen proteins were identified in grains, using 2-DE coupled with mass spectrometry, with higher expression in Zhenong8 than that in W6nk2; including z-type serpin, serpin-Z7 and alpha-amylase/trypsin inhibitor CM, carbohydrate metabolism, protein synthesis and signal transduction related proteins. Twelve proteins were less expressed in Zhenong8 than that in W6nk2; including barley trypsin inhibitor chloroform/methanol-soluble protein (BTI-CMe2.1, BTI-CMe2.2, trypsin inhibitor, dehydroascorbate reductase (DHAR, pericentrin, dynein heavy chain and some antiviral related proteins. The data extend our understanding of mechanisms underlying Cd accumulation/tolerance and provides possible utilization of elite genetic resources in developing low-grain-Cd barley cultivars.

  3. Good Grains Gone Bad: How Grain to Grain Interactions Complicate the Onset of Motion

    Science.gov (United States)

    Yager, E.; Schmeeckle, M. W.

    2015-12-01

    Predictions of the onset of sediment motion are integral components of bed stability and bedload flux estimates. Mechanistic equations for initial motion employ a balance between driving and resisting forces. Driving forces are modeled as functions of the magnitude and duration of turbulence events whereas resisting forces are simply approximated by the grain weight and a static friction angle. Such resistance approximations do not include the effects of grain packing and dynamic interactions with surrounding sediment. To better understand and quantify grain resistance, we used a Discrete Element Method (DEM) model for a single test grain surrounded by a bed of smaller grains. We applied a constant external force on the test grain in each run and progressively increased the force between runs until the test grain moved out of its resting pocket. The DEM model calculated the test grain velocity, position and net force (sum of applied external force and forces from other grains) at time steps of 1×10-7 s. Despite applying a constant external force, the net force on the test grain fluctuated by three to six orders of magnitude, depending on the run. These fluctuations were driven by the creation and destruction of force chains, and the rearrangement of the positions of surrounding bed sediment. Stick-slip behavior, which has been observed in shear tests of granular material, occurred during test-grain motion. The frequency of stick-slip behavior generally declined with higher applied external forces. Therefore, the onset of grain motion was not continuous, as is often assumed even in the presence of fluctuating applied fluid forces. The duration and magnitude of turbulence fluctuations have received considerable attention but our results suggest that grain resistance oscillations are also important. Whether turbulence and resistance fluctuations are synchronous will likely dictate if grain movement occurs, and we are currently conducting model runs to better

  4. Selective filling of Photonic Crystal Fibres

    DEFF Research Database (Denmark)

    Nielsen, Kristian; Noordegraaf, Danny; Sørensen, Thorkild

    2005-01-01

    A model for calculating the time necessary for filling one or more specific holes in a photonic crystal fibre is made. This model is verified for water, and its enabling potential is illustrated by a polymer application. Selective filling of the core in an air-guide photonic crystal fibre is demo...

  5. Influence of template fill in graphoepitaxy DSA

    Science.gov (United States)

    Doise, Jan; Bekaert, Joost; Chan, Boon Teik; Hong, SungEun; Lin, Guanyang; Gronheid, Roel

    2016-03-01

    Directed self-assembly (DSA) of block copolymers (BCP) is considered a promising patterning approach for the 7 nm node and beyond. Specifically, a grapho-epitaxy process using a cylindrical phase BCP may offer an efficient solution for patterning randomly distributed contact holes with sub-resolution pitches, such as found in via and cut mask levels. In any grapho-epitaxy process, the pattern density impacts the template fill (local BCP thickness inside the template) and may cause defects due to respectively over- or underfilling of the template. In order to tackle this issue thoroughly, the parameters that determine template fill and the influence of template fill on the resulting pattern should be investigated. In this work, using three process flow variations (with different template surface energy), template fill is experimentally characterized as a function of pattern density and film thickness. The impact of these parameters on template fill is highly dependent on the process flow, and thus pre-pattern surface energy. Template fill has a considerable effect on the pattern transfer of the DSA contact holes into the underlying layer. Higher fill levels give rise to smaller contact holes and worse critical dimension uniformity. These results are important towards DSA-aware design and show that fill is a crucial parameter in grapho-epitaxy DSA.

  6. Re-partitioning of Non-structural Carbohydrates in Rice Stems and Their Roles in Yield Stability and Stress Tolerance%水稻茎鞘非结构性碳水化合物再分配及其在稳产和抗逆中的作用

    Institute of Scientific and Technical Information of China (English)

    潘俊峰; 李国辉; 崔克辉

    2014-01-01

    High and stable grain yield of rice under changeable environments is significant for meeting the increasing food demand due to the rapid population growth.When assimilates are insufficient due to lower photosynthetic capability during the grain filling stage or under abiotic stresses,non-structural carbohydrates (NSCs)stored in the rice stems (leaf sheath and culm)may be as a source of carbohydrates for grain filling and relief of grain yield reduction. Considering the importance of NSCs,traits related to NSCs accumulation and re-partitioning are becoming a target for genetically improving grain yield and stress resistance in rice, and some attempts are made to regulate NSCs accumulation and re-partitioning by integrated crop management.We review effects of several factors on NSCs accumulation and re-partitioning,such as characteristics of source-flow-sink,nitrogen and water application,light density,ambient temperature,lodging resistance.On the other hand,we evaluate the regulation of NSCs accumulation and re-partitioning by optimization of nitrogen fertilizer application,irrigation and transplanting density.Additionally, roles of stem NSCs in increasing grain yield stability and stress resistance are discussed.%为了满足人口增长对粮食的需求,保持水稻高产稳产和适应多变的环境显得尤为重要。水稻茎鞘储藏的非结构性碳水化合物(NSCs)可以在灌浆期光合受阻、非生物逆境胁迫时为产量形成提供同化物,从而缓解产量的降低。因此,茎鞘NSCs 积累转运性状是水稻产量形成和逆境抗性遗传改良的潜在目标性状,调控茎鞘 NSCs 的积累与分配也成为水稻肥水管理的一个重要目的。综述了水稻源-库-流特征、氮肥、水分、光强、环境温度和水稻抗倒伏能力与茎鞘储藏 NSCs 积累与再分配的关系,氮肥运筹、合理灌溉、栽插密度等栽培措施对茎鞘 NSCs 积累与再分配的调控。最后,讨论了茎鞘 NSCs

  7. Germinated grains: a superior whole grain functional food?

    Science.gov (United States)

    Nelson, Kristina; Stojanovska, Lily; Vasiljevic, Todor; Mathai, Michael

    2013-06-01

    Grains are global dietary staples that when consumed in whole grain form, offer considerable health benefits compared with milled grain foods, including reduced body weight gain and reduced cardiovascular and diabetes risks. Dietary patterns, functional foods, and other lifestyle factors play a fundamental role in the development and management of epidemic lifestyle diseases that share risks of developing adverse metabolic outcomes, including hyperglycaemia, hypertension, dyslipidaemia, oxidative stress, and inflammation. Whole grains provide energy, nutrients, fibres, and bioactive compounds that may synergistically contribute to their protective effects. Despite their benefits, the intake of grains appears to be lower than recommended in many countries. Of emerging interest is the application of germination processes, which may significantly enhance the nutritional and bioactive content of grains, as well as improve palatability. Enhancing grain foods in a natural way using germination techniques may therefore offer a practical, natural, dietary intervention to increase the health benefits and acceptability of whole grains, with potentially widespread effects across populations in attenuating adverse lifestyle disease outcomes. Continuing to build on the growing body of in-vitro studies requires substantiation with extended in-vivo trials so that we may further develop our understanding of the potential of germinated grains as a functional food.

  8. [Malabsorption of carbohydrates in children (author's transl)].

    Science.gov (United States)

    Vázquez, C; Escobar, H; Polanco, I; Codoceo, R; Vitoria, J C

    1975-01-01

    Physiological bases of digestion and absorption of carbohydrates are reviewed, as a preliminary step, in order to draw a general scheme of its patholophysiology. Clasification of different types of carbohydrate malabsorption is presented. Various exploration methods are discussed in terms of autors' own experiences. Relationship between a sugar screening test, faecal lactic acid contents and a simplified lactose tolerance test, is described in detail. Systematic diagnoses of these diseases are established. Different clinical pictures are reviewed. It is not yet well defined if a starch malabsorption can be caused by either a primary or secondary duodenal amylase deficiency. The clinical forms of congenital sucrose-isomaltose intolerance may be more attenuated than its classical form; incertain cases, secondary sucrose intolerance may also be present due to mucosa anatomic lesions. Maltose malabsorption has no clinical implications. As compared to other alpha-glycosidades, the trehalase activity has been not more affected by not using trehalose in feeding. Primary congenital lactase deficiency is not frequent, whereas secondary forms as much more usual and appear, in primary malabsorption syndromes and in the coeliac disease, very often along with clinical tolerance to lactose. In Spain, lactose nonabsorbers in 16.5% for adults 11.2% for adolescents and 18.3% for children, meaning, that it is being favored by environmental factors in the latter. The unspecified sugar malabsorption during the child's first year is still the most frequent cause of carbohydrate intolerance in children and, although certain progress has been achieved in its diagnosis and therapy, its pathogenic mechanism is not satisfactorily known yet.

  9. Carbohydrate metabolism of rats with biliary obstruction.

    Directory of Open Access Journals (Sweden)

    Kim,Hitoshi

    1990-08-01

    Full Text Available Carbohydrate metabolism of rats with obstructive jaundice caused by bile duct ligation was studied by intravenous glucose tolerance test (IVGTT and by liver perfusion. The altered levels of carbohydrate-metabolizing enzyme were examined in relation to the glucose metabolism of the cholestatic rats. In the IVGTT, the rate of fractional glucose removal was increased with increases in plasma insulin and glucagon and with a decrease in non-esterified fatty acid. In liver perfusion, neither the glucose uptake nor insulin extraction by the whole liver of icteric rats was different from the control. The increased rate of glucose removal in IVGTT may be due to enhanced glucose utilization by peripheral tissues resulting from hypersecretion of insulin. In liver perfusate supplemented with glucose, a decrease in the glucose uptake per unit liver weight was observed in relation to the lowered glucokinase activity. Formation of glycogen from glucose and of glucose from lactate was also impaired, indicating inhibition of the gluconeogenic system or relative hyperfunction of the glycolytic system, which may further contribute to the reduction in glycogen content. These metabolic disorders correlated well with the changes in activities of key carbohydrate-metabolizing enzymes, which showed a characteristic pattern consistent with the loss of differentiated hepatic functions. Uptake of glucose and its conversion to glycogen were reduced in the cholestatic liver in close association with altered activities of some of related enzymes. However, due to increased utilization by the peripheral tissues, the total amount of glucose utilized in the whole rat was not reduced.

  10. Changes in fatty acid content and composition in silage maize during grain filling

    NARCIS (Netherlands)

    Khan, N.A.; Cone, J.W.; Pellikaan, W.F.; Khan, M.A.; Struik, P.C.; Hendriks, W.H.

    2011-01-01

    Background: The stage of maturity at harvest has a major effect on the fatty acid (FA) content and composition of forage plants consumed by dairy cows. The present study investigated the dynamics of FA content and composition in stover (leaves and stem) and ears (cob, shank and husks) of two maize g

  11. Exogenous abscisic acid application during grain filling in winter wheat improves cold tolerance of offspring's seedlings

    DEFF Research Database (Denmark)

    Li, X.; Cai, J.; Liu, Fulai

    2014-01-01

    Low temperature seriously depresses seed germination and seedling growth in winter wheat (Triticum aestivum L.). In this study, wheat plants were sprayed with abscisic acid (ABA) and fluridone (inhibitor of ABA biosynthesis) at 19 days after anthesis (DAA) and repeated at 26 DAA. The seeds of those...... plants were harvested, and seed germination and offspring's seedling growth under low temperature were evaluated. The results showed that exogenous ABA application decreased seed weight and slightly reduced seed set and seed number per spike. Under low temperature, seeds from ABA-treated plants showed...... reduced germination rate, germination index, growth of radicle and coleoptile, amylase activity and depressed starch degradation as compared with seeds from non-ABA-treated plants; however, activities of the antioxidant enzymes in both germinating seeds and seedling were enhanced from those exposed...

  12. Carbohydrate nanotechnology: hierarchical assembly using nature's other information carrying biopolymers.

    Science.gov (United States)

    Han, Xu; Zheng, Yeting; Munro, Catherine J; Ji, Yiwen; Braunschweig, Adam B

    2015-08-01

    Despite their central role in directing some of the most complex biological processes, carbohydrates--nature's other information carrying biopolymer--have been largely ignored as building blocks for synthetic hierarchical assemblies. The non-stoichiometric binding and astronomical diversity characteristic of carbohydrates could lead to tantalizingly complex assembly algorithms, but these attributes simultaneously increase the difficulty of preparing carbohydrate assemblies and anticipating their behavior. Convergences in biotechnology, nanotechnology, polymer chemistry, surface science, and supramolecular chemistry have led to many recent important breakthroughs in glycan microarrays and synthetic carbohydrate receptors, where the idiosyncrasies of carbohydrate structure and binding are increasingly considered. We hope to inspire more researchers to consider carbohydrate structure, diversity, and binding as attractive tools for constructing synthetic hierarchical assemblies.

  13. Carbohydrates/nucleosides/RNA-DNA-ligand interactions

    Energy Technology Data Exchange (ETDEWEB)

    Kaptein, R.; McConnell, B.; Serianni, A.S.; Silks, L.A. III

    1994-12-01

    Carbohydrate and nucleotide structural determination using modern spectroscopic techniques is dependent on our ability to label oligonucleotides and oligosaccharides with stable isotopes. Uniform Carbon 13 and Nitrogen 15 labeling of oligonucleotides is important to present-day efforts, which are focused on determining the structure of relatively small oligosaccharides and oligonucleotides, which form the elements of larger structures. Because of the relatively recent interest in three-dimensional structure, the development of techniques used to label them has lagged behind parallel techniques used to label peptides and proteins. Therefore, this group`s discussion focused primarily on problems faced today in obtaining oligonucleotides labeled uniformly with carbon 13 and nitrogen 15.

  14. A high-power carbohydrate fuel cell

    Energy Technology Data Exchange (ETDEWEB)

    Larsson, Ragnar [SuFuCell AB, Bytaregatan 23, SE 222 21 Lund (Sweden); Folkesson, Boerje [Bronsaaldersvaegen 21, SE-226 54 Lund (Sweden); Spaziante, Placido M. [Cellennium Co., Ltd., 14th Floor Gypsum Metropolitan Tower, 539 Sri Ayudhaya Rd., Bangkok 10400 (Thailand); Veerasai, Waret [Chemistry Department, Faculty of Science, Mahidol University, Bangkok 10400 (Thailand); Exell, Robert H.B. [Joint Graduate School of Energy and Environment, King Mongkut' s University of Technology Thonburi, 91 Prachauthit Rd., Bangmod, Tungkru, Bangkok 10140 (Thailand)

    2006-04-01

    This paper reports the development of a fuel cell consisting of a vanadium flow battery in which the vanadium ions are reduced by sugar (from a carbohydrate) to oxidation state +3 on one side of a membrane, and are oxidized to state +5 on the other side by oxygen. The theoretical upper limit to the conversion efficiency of the energy in sugar by this method under standard conditions is 54%. We have obtained efficiencies up to 45% in our laboratory tests. This way of using biomass for electricity production avoids the Carnot cycle losses in heat engines. (author)

  15. Grain charging in protoplanetary discs

    CERN Document Server

    Ilgner, Martin

    2011-01-01

    Recent work identified a growth barrier for dust coagulation that originates in the electric repulsion between colliding particles. Depending on its charge state, dust material may have the potential to control key processes towards planet formation such as MHD (magnetohydrodynamic) turbulence and grain growth which are coupled in a two-way process. We quantify the grain charging at different stages of disc evolution and differentiate between two very extreme cases: compact spherical grains and aggregates with fractal dimension D_f = 2. Applying a simple chemical network that accounts for collisional charging of grains, we provide a semi-analytical solution. This allowed us to calculate the equilibrium population of grain charges and the ionisation fraction efficiently. The grain charging was evaluated for different dynamical environments ranging from static to non-stationary disc configurations. The results show that the adsorption/desorption of neutral gas-phase heavy metals, such as magnesium, effects the ...

  16. The polystyrene microsphere filling with hydrogen isotopes through the fill tube with consequent freezing

    Science.gov (United States)

    Izgorodin, V. M.; Solomatina, E. Y.; Pepelyaev, A. P.; Rogozhina, M. A.; Osetrov, E. I.

    2016-09-01

    Process of spherical polystyrene capsules filling with hydrogen isotopes through the fill tube for the purpose of a cryogenic target building is described. The scheme of the stand for researches and a technique of carrying out of experiments is represented. Results of capsules filling and subsequent freezing for protium, deuterium and protium- deuterium mixture are shown.

  17. Terpenes and carbohydrate source influence rumen fermentation, digestibility, intake, and preference in sheep.

    Science.gov (United States)

    Villalba, J J; Provenza, F D; Olson, K C

    2006-09-01

    We hypothesized that toxins and nutrients in foods interact to influence foraging behavior by herbivores. Based on this hypothesis we predicted that 1) terpenes in big sagebrush (Artemisia tridentata) influence intake and preference in sheep for diets varying in sources of nonstructural (barley grain) and structural (sugar beet pulp) carbohydrates, and 2) these effects are due to the differential effects of terpenes on fermentation products and apparent digestibility of each class of carbohydrates. Lambs were fed 2 isoenergetic and isonitrogenous diets with varying proportions of the same ingredients (beet pulp- and barley grain-based diet) or offered a choice between the 2 diets; all feeds were fed without and with terpenes, in consecutive periods. We also compared intake and preference of the beet pulp-and barley-based diets before and after the lambs ate a meal of sagebrush. Finally, we assessed the effect of terpenes on ruminal variables and in vivo digestibility. Lambs ate less when fed beet pulp or when they were offered a choice of diets with terpenes (P 0.20) before eating sagebrush, but they preferred the beet pulp-based diet after eating sagebrush (P feed (P = 0.63). In summary, the predominant type of feed ingredient (beet pulp, grain) ingested with terpenes influenced fermentation products, intake, and preference in lambs. The source of energy from supplements, or other plants in the diet, is likely to influence intake and preference for sagebrush in sheep foraging on rangelands. Moreover, ingesting terpenes from sagebrush may also influence intake and preference for other plant species or supplements.

  18. Differences among Elite Female Rowers Regarding Carbohydrate Consumption at Rest

    OpenAIRE

    BADAU, Dana; TOMESCU, VALERIU; BADAU, Adela; MARTIN, Ștefan Adrian

    2016-01-01

    Backround: The objective of this study was interpreting the reported differences within carbohydrate consumption at rest, in female rowing groups. Method: We conducted a cross-sectional study, on a group of elite rowing athletes, monitoring carbohydrate consumption at rest. 34 subjects, divided in 3 groups of activities (senior, youth, junior) took part in this study being monitored through Cosmed Quark CPET device. Results: The average amount of carbohydrates consumed at rest among the group...

  19. Structure-function relations of carbohydrates by neoglycolipid arrays.

    Science.gov (United States)

    Huang, Gang-Liang; Huang, Hua-Liang; Zhang, Hou-Cheng; Wang, Peng-George

    2006-06-01

    The work presented herein is a new noncovalent glycoarray assembly method for microplates created by simply mixing together a carbohydrate and a tetradecylamine. alpha-D-Mannopyranoside, alpha-D-glucopyranoside, and alpha-D-galactopyranoside were utilized in model studies and product formations were detected by lectin binding. The method can be extended to study the steric hindrance effect of carbohydrate-protein interactions, namely the structure-function relations of carbohydrates.

  20. CARBOHYDRATE INGESTION AND EXERCISE: EFFECTS ON METABOLISM AND PERFORMANCE

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    @@KEY POINTS ■ Carbohydrate is the preferred fuel for most competitive sports;an inadequate supply of carbohydrate in the body often leads to poor performance. ■ Carbohydrate ingestion during exercise increases blood glucose availability and maintains the ability of the body to use carbohydrate as fuel during exercise.When carbohydrate is consumed during exercise,glucose uptake by muscles is increased,and the breakdown of glycogen in the liver into blood glucose is reduced,thus saving liver glycogen until late in exercise.The use of muscle glycogen for energy is generally unaffected by carbohydrate feeding.However,during prolonged running,the breakdown of muscle glycogen may be slowed because the supply of blood glucose is improved when carbohydrate is consumed.These metabolic responses underlie the performance benefit that accompanies carbohydrate ingestion during exercise. ■ There are some minor differences among glucose,sucrose,and maltodextrins in their effects on metabolism,but each of them can enhance performance when ingested in the appropriate quantity during exercise.Fructose alone is not an effective carbohydrate supplement because of its slow absorption and slow conversion by the body to glucose,but when small amounts of fructose are combined with other carbohydrates,fructose can be beneficial. ■ Ingesting carbohydrate at a rate of 30-60 grams per hour can improve exercise erformance.A good way to achieve this carbohydrate intake is to consume 600-to-1200 ml(20-to-40 oz)of a sports drink during each hour of exercise.Consuming carbohydrate in a beverage provides an added benefit of preventing potentially harmful effects of dehydration on performance.

  1. Carbohydrate-active enzymes from pigmented Bacilli: a genomic approach to assess carbohydrate utilization and degradation

    Directory of Open Access Journals (Sweden)

    Henrissat Bernard

    2011-09-01

    Full Text Available Abstract Background Spore-forming Bacilli are Gram-positive bacteria commonly found in a variety of natural habitats, including soil, water and the gastro-intestinal (GI-tract of animals. Isolates of various Bacillus species produce pigments, mostly carotenoids, with a putative protective role against UV irradiation and oxygen-reactive forms. Results We report the annotation of carbohydrate active enzymes (CAZymes of two pigmented Bacilli isolated from the human GI-tract and belonging to the Bacillus indicus and B. firmus species. A high number of glycoside hydrolases (GHs and carbohydrate binding modules (CBMs were found in both isolates. A detailed analysis of CAZyme families, was performed and supported by growth data. Carbohydrates able to support growth as the sole carbon source negatively effected carotenoid formation in rich medium, suggesting that a catabolite repression-like mechanism controls carotenoid biosynthesis in both Bacilli. Experimental results on biofilm formation confirmed genomic data on the potentials of B. indicus HU36 to produce a levan-based biofilm, while mucin-binding and -degradation experiments supported genomic data suggesting the ability of both Bacilli to degrade mammalian glycans. Conclusions CAZy analyses of the genomes of the two pigmented Bacilli, compared to other Bacillus species and validated by experimental data on carbohydrate utilization, biofilm formation and mucin degradation, suggests that the two pigmented Bacilli are adapted to the intestinal environment and are suited to grow in and colonize the human gut.

  2. Overexpression of a NAC transcription factor delays leaf senescence and increases grain nitrogen concentration in wheat.

    Science.gov (United States)

    Zhao, D; Derkx, A P; Liu, D-C; Buchner, P; Hawkesford, M J

    2015-07-01

    Increasing the duration of leaf photosynthesis during grain filling using slow-senescing functional stay-green phenotypes is a possible route for increasing grain yields in wheat (Triticum aestivum L.). However, delayed senescence may negatively affect nutrient remobilisation and hence reduce grain protein concentrations and grain quality. A novel NAC1-type transcription factor (hereafter TaNAC-S) was identified in wheat, with gene expression located primarily in leaf/sheath tissues, which decreased during post-anthesis leaf senescence. Expression of TaNAC-S in the second leaf correlated with delayed senescence in two doubled-haploid lines of an Avalon × Cadenza population (lines 112 and 181), which were distinct for leaf senescence. Transgenic wheat plants overexpressing TaNAC-S resulted in delayed leaf senescence (stay-green phenotype). Grain yield, aboveground biomass, harvest index and total grain N content were unaffected, but NAC over-expressing lines had higher grain N concentrations at similar grain yields compared to non-transgenic controls. These results indicate that TaNAC-S is a negative regulator of leaf senescence, and that delayed leaf senescence may lead not only to increased grain yields but also to increased grain protein concentrations.

  3. Grain Handling and Storage Safety

    OpenAIRE

    Webster, Jill, Ph.D.

    2005-01-01

    Agricultural Health and Safety Fact Sheet AHS-02 Grain Handling and Storage Safety Jill Webster Ph.D., S. Christian Mariger, Graduate Assistant Agricultural Systems Technology and Education There are several hazards that should be considered when working with grain. Storage structures, handling equipment, and the grain itself have all caused serious injuries and deaths. Storage structures (bins, silos, and granaries), like all confined spaces, have significant hazards associated with them. Be...

  4. The least-cost low-carbohydrate diet is expensive.

    Science.gov (United States)

    Raffensperger, John F

    2008-01-01

    This article describes the use of operations research methods to study the minimum possible cost of a low-carbohydrate diet. The study compares this cost to the minimum cost of a diet with no limitation on carbohydrate. The rationale for this study is the popularity of the low-carbohydrate diets and their perceived high cost. The method used was an operations research approach to find a set of least cost diets, varying the required carbohydrate. This method was chosen to avoid potential concerns with real diets that may be nutritionally deficient or could be had for a lower cost. The major finding is that the cheapest possible low-carbohydrate diet costs about triple the cost of the cheapest diet with no constraint on carbohydrate. Furthermore, the minimum cost of a diet low in both carbohydrate and fat is 5 to 10 times the cost of the cheapest diet, depending on the relative amounts of these nutrients. As carbohydrate and fat are constrained, cost increases dramatically and nonlinearly. The study identifies which nutrients had the greatest effect on cost for a low-carbohydrate and low-fat diet.

  5. Droplet Measurement below Single-Layer Grid Fill

    Directory of Open Access Journals (Sweden)

    Vitkovic Pavol

    2016-01-01

    Full Text Available The main part of the heat transfer in a cooling tower is in a fill zone. This one is consist of a cooling fill. For the cooling tower is used a film fill or grid fill or splash fill in the generally. The grid fill has lower heat transfer performance like film fill usually. But their advantage is high resistance to blockage of the fill. The grid fill is consisted with independent layers made from plastic usually. The layers consist of several bars connected to the different shapes. For experiment was used the rhombus shape. The drops diameter was measured above and below the Grid fill.

  6. Renewable Hydrogen Carrier — Carbohydrate: Constructing the Carbon-Neutral Carbohydrate Economy

    Directory of Open Access Journals (Sweden)

    Y.-H. Percival Zhang

    2011-01-01

    Full Text Available The hydrogen economy presents an appealing energy future but its implementation must solve numerous problems ranging from low-cost sustainable production, high-density storage, costly infrastructure, to eliminating safety concern. The use of renewable carbohydrate as a high-density hydrogen carrier and energy source for hydrogen production is possible due to emerging cell-free synthetic biology technology—cell-free synthetic pathway biotransformation (SyPaB. Assembly of numerous enzymes and co-enzymes in vitro can create complicated set of biological reactions or pathways that microorganisms or catalysts cannot complete, for example, C6H10O5 (aq + 7 H2O (l à 12 H2 (g + 6 CO2 (g (PLoS One 2007, 2:e456. Thanks to 100% selectivity of enzymes, modest reaction conditions, and high-purity of generated hydrogen, carbohydrate is a promising hydrogen carrier for end users. Gravimetric density of carbohydrate is 14.8 H2 mass% if water can be recycled from proton exchange membrane fuel cells or 8.33% H2 mass% without water recycling. Renewable carbohydrate can be isolated from plant biomass or would be produced from a combination of solar electricity/hydrogen and carbon dioxide fixation mediated by high-efficiency artificial photosynthesis mediated by SyPaB. The construction of this carbon-neutral carbohydrate economy would address numerous sustainability challenges, such as electricity and hydrogen storage, CO2 fixation and long-term storage, water conservation, transportation fuel production, plus feed and food production.

  7. CARBOHYDRATE INTAKE CONSIDERATIONS FOR YOUNG ATHLETES

    Directory of Open Access Journals (Sweden)

    Veronica Montfort-Steiger

    2007-09-01

    Full Text Available Good nutritional practices are important for exercise performance and health during all ages. Athletes and especially growing children engaged in heavy training have higher energy and nutrient requirements compared to their non-active counterparts. Scientific understanding of sports nutrition for the young athlete is lacking behind the growing number of young athletes engaged in sports. Most of the sports nutrition recommendations given to athletic children and adolescents are based on adult findings due to the deficiency in age specific information in young athletes. Therefore, this review reflects on child specific sports nutrition, particularly on carbohydrate intake and metabolism that distinguishes the child athlete from the adult athlete. Children are characterised to be in an insulin resistance stage during certain periods of maturation, have different glycolytic/metabolic responses during exercise, have a tendency for higher fat oxidation during exercise and show different heat dissipation mechanisms compared to adults. These features point out that young athletes may need different nutritional advice on carbohydrate for exercise to those from adult athletes. Sport drinks for example may need to be adapted to children specific needs. However, more research in this area is warranted to clarify sports nutrition needs of the young athlete to provide better and healthy nutritional guidance to young athletes

  8. Fine-grained sediment dispersal along the California coast

    Science.gov (United States)

    Warrick, Jonathan A.; Storlazzi, Curt D.

    2013-01-01

    Fine-grained sediment (silt and clay) enters coastal waters from rivers, eroding coastal bluffs, resuspension of seabed sediment, and human activities such as dredging and beach nourishment. The amount of sediment in coastal waters is an important factor in ocean ecosystem health, but little information exists on both the natural and human-driven magnitudes of fine-grained sediment delivery to the coastal zone, its residence time there, and its transport out of the system—information upon which to base environmental assessments. To help fill these information gaps, the U.S. Geological Survey has partnered with Federal, State, and local agencies to monitor fine-grained sediment dispersal patterns and fate in the coastal regions of California. Results of these studies suggest that the waves and currents of many of the nearshore coastal settings of California are adequately energetic to transport fine-grained sediment quickly through coastal systems. These findings will help with the management and regulation of fine-grained sediment along the U.S. west coast.

  9. Oil and Protein Accumulation in Soybean Grains under Salinity Stress

    Directory of Open Access Journals (Sweden)

    Kazem GHASSEMI-GOLEZANI

    2010-06-01

    Full Text Available Two factorial experiments based on randomized complete block design (RCBD with three replications were conducted in 2007 and 2008, to evaluate grain development (four harvests and rate and duration of oil and protein accumulation in three soybean cultivars (�Williams�, �Zan� and �L17� under a non-saline (control and three saline (3, 6 and 9 ds/m NaCl conditions. Six seeds were sown in each pot filled with 900 g perlite, using 144 pots for each experiment. After emergence, seedlings were thinned and 4 plants were kept in each pot. Rate of oil accumulation up to maturity was not significantly affected by salinity. With increasing salinity, rate and percentage of protein accumulation, duration of oil and protein accumulation and oil and protein content per grain decreased, but oil percentage increased. Oil and protein yields per plant decreased as salinity increased. These reductions were mainly attributed to the short duration of protein and oil accumulation and grain yield per plant under saline conditions. �Williams� had the highest rate and duration of protein accumulation and rate of oil accumulation, but �L17� had the highest grain yield per plant. Consequently, differences in protein and oil yields per plant between these two cultivars were not statistically significant. However, �Zan� had the lowest protein and oil yields, due to the lowest grain yield per plant.

  10. Whole grains, legumes, and the subsequent meal effect: implications for blood glucose control and the role of fermentation.

    Science.gov (United States)

    Higgins, Janine A

    2012-01-01

    Whole grains and legumes are known to reduce postprandial glycemia and, in some instances, insulinemia. However, the subsequent meal effect of ingesting whole grains and legumes is less well known. That is, inclusion of whole grains or legumes at breakfast decreases postprandial glycemia at lunch and/or dinner on the same day whereas consumption of a whole grain or lentil dinner reduces glycemia at breakfast the following morning. This effect is lost upon milling, processing, and cooking at high temperatures. The subsequent meal effect has important implications for the control of day-long blood glucose, and may be partly responsible for the reduction in diabetes incidence associated with increased whole grain and legume intake. This paper describes the subsequent meal effect and explores the role of acute glycemia, presence of resistant starch, and fermentation of indigestible carbohydrate as the mechanisms responsible for this effect.

  11. Whole Grains, Legumes, and the Subsequent Meal Effect: Implications for Blood Glucose Control and the Role of Fermentation

    Directory of Open Access Journals (Sweden)

    Janine A. Higgins

    2012-01-01

    Full Text Available Whole grains and legumes are known to reduce postprandial glycemia and, in some instances, insulinemia. However, the subsequent meal effect of ingesting whole grains and legumes is less well known. That is, inclusion of whole grains or legumes at breakfast decreases postprandial glycemia at lunch and/or dinner on the same day whereas consumption of a whole grain or lentil dinner reduces glycemia at breakfast the following morning. This effect is lost upon milling, processing, and cooking at high temperatures. The subsequent meal effect has important implications for the control of day-long blood glucose, and may be partly responsible for the reduction in diabetes incidence associated with increased whole grain and legume intake. This paper describes the subsequent meal effect and explores the role of acute glycemia, presence of resistant starch, and fermentation of indigestible carbohydrate as the mechanisms responsible for this effect.

  12. Acute Effects of Carbohydrate Supplementation on Intermittent Sports Performance

    Directory of Open Access Journals (Sweden)

    Lindsay B. Baker

    2015-07-01

    Full Text Available Intermittent sports (e.g., team sports are diverse in their rules and regulations but similar in the pattern of play; that is, intermittent high-intensity movements and the execution of sport-specific skills over a prolonged period of time (~1–2 h. Performance during intermittent sports is dependent upon a combination of anaerobic and aerobic energy systems, both of which rely on muscle glycogen and/or blood glucose as an important substrate for energy production. The aims of this paper are to review: (1 potential biological mechanisms by which carbohydrate may impact intermittent sport performance; (2 the acute effects of carbohydrate ingestion on intermittent sport performance, including intermittent high-intensity exercise capacity, sprinting, jumping, skill, change of direction speed, and cognition; and (3 what recommendations can be derived for carbohydrate intake before/during exercise in intermittent sports based on the available evidence. The most researched intermittent sport is soccer but some sport-specific studies have also been conducted in other sports (e.g., rugby, field hockey, basketball, American football, and racquet sports. Carbohydrate ingestion before/during exercise has been shown in most studies to enhance intermittent high-intensity exercise capacity. However, studies have shown mixed results with regards to the acute effects of carbohydrate intake on sprinting, jumping, skill, change of direction speed, and cognition. In most of these studies the amount of carbohydrate consumed was ~30–60 g/h in the form of a 6%–7% carbohydrate solution comprised of sucrose, glucose, and/or maltodextrin. The magnitude of the impact that carbohydrate ingestion has on intermittent sport performance is likely dependent on the carbohydrate status of the individual; that is, carbohydrate ingestion has the greatest impact on performance under circumstances eliciting fatigue and/or hypoglycemia. Accordingly, carbohydrate ingestion before

  13. Saturated Fat as Compared With Unsaturated Fats and Sources of Carbohydrates in Relation to Risk of Coronary Heart Disease: A Prospective Cohort Study

    Science.gov (United States)

    Bernstein, Adam M.; Ley, Sylvia H.; Wang, Dong D.; Chiuve, Stephanie E.; Sampson, Laura; Rexrode, Kathryn M.; Rimm, Eric B.; Willett, Walter C.; Hu, Frank B.

    2015-01-01

    Background The associations between dietary saturated fat and risk of coronary heart disease (CHD) remain controversial, but few studies have compared saturated with unsaturated fats and sources of carbohydrates in relation to CHD risk. Objective This study sought to investigate associations of saturated fats as compared with unsaturated fats and different sources of carbohydrates in relation to CHD risk. Methods We followed 84,628 women (Nurses’ Health Study, 1980 to 2010), and 42,908 men (Health Professionals Follow-up Study, 1986 to 2010) who were free of diabetes, cardiovascular disease, and cancer at baseline. Diet was assessed by semiquantitative food frequency questionnaire every 4 years. Results During 24 to 30 years of follow-up, we documented 7,667 incident cases of CHD. Higher intakes of polyunsaturated fatty acids (PUFAs) and carbohydrates from whole grains were significantly associated with lower risk of CHD (hazard ratios [HR] (95% confidence intervals [CI]) comparing the highest to the lowest quintile for PUFA: 0.80 [0.73 to 0.88], p trend saturated fats with equivalent energy intake from either PUFAs, monounsaturated fats (MUFAs), or carbohydrates from whole grains was associated with 25%, 15%, and 9% lower risk of CHD, respectively (PUFAs: 0.75 [0.67 to 0.84]; p saturated fat with carbohydrates from refined starches/added sugars was not significantly associated with CHD risk (p > 0.10). Conclusions Our findings indicate that unsaturated fats, especially PUFAs, and/or high-quality carbohydrates should replace dietary saturated fats to reduce CHD risk. PMID:26429077

  14. The effects of nitrogen uptake before and after heading on grain protein content and the occurrence of basal- and back-white grains in rice (Oryza sativa L.

    Directory of Open Access Journals (Sweden)

    Tadashi Tsukaguchi

    2016-10-01

    Full Text Available Chalky rice (Oryza sativa L. grains are induced by high temperature during the grain-filling period. Plant nitrogen status also affects the occurrence of basal- and back-white grains (BBWG. The objective of this study was to elucidate the relations between nitrogen availability per spikelet during the grain-filling period (NGF and each of the percentage of BBWG and grain protein content (GPC. We further compared the effect of the components of NGF determined before heading (NBH and after heading (NAH on BBWG and GPC. We grew the rice cultivar ‘Koshihikari’ in pots in 2012 and 2013, and top-dressed nitrogen at the panicle formation and heading stages, under two (2012 or three (2013 temperature regimes during the grain-filling period. GPC was explained well by NGF, but BBWG was not. BBWG was best explained in a multiple-regression equation by mean air temperature after heading and by NBH and NAH. The partial correlation coefficients for NBH were 1.6 and 3.0 times those for NAH in 2012 and 2013, respectively. On the other hand, in a multiple-regression equation for GPC, the partial correlation coefficients for NBH were 0.91 and 0.71 times those for NAH in 2012 and 2013, respectively. These results suggest that rice grains are most sensitive to plant nitrogen status before heading for BBWG but after heading for GPC, and that there is an optimal timing for nitrogen top-dressing that would maximize the reduction in BBWG per unit increment of GPC.

  15. The use of carbohydrates during exercise as an ergogenic aid.

    Science.gov (United States)

    Cermak, Naomi M; van Loon, Luc J C

    2013-11-01

    Carbohydrate and fat are the two primary fuel sources oxidized by skeletal muscle tissue during prolonged (endurance-type) exercise. The relative contribution of these fuel sources largely depends on the exercise intensity and duration, with a greater contribution from carbohydrate as exercise intensity is increased. Consequently, endurance performance and endurance capacity are largely dictated by endogenous carbohydrate availability. As such, improving carbohydrate availability during prolonged exercise through carbohydrate ingestion has dominated the field of sports nutrition research. As a result, it has been well-established that carbohydrate ingestion during prolonged (>2 h) moderate-to-high intensity exercise can significantly improve endurance performance. Although the precise mechanism(s) responsible for the ergogenic effects are still unclear, they are likely related to the sparing of skeletal muscle glycogen, prevention of liver glycogen depletion and subsequent development of hypoglycemia, and/or allowing high rates of carbohydrate oxidation. Currently, for prolonged exercise lasting 2-3 h, athletes are advised to ingest carbohydrates at a rate of 60 g·h⁻¹ (~1.0-1.1 g·min⁻¹) to allow for maximal exogenous glucose oxidation rates. However, well-trained endurance athletes competing longer than 2.5 h can metabolize carbohydrate up to 90 g·h⁻¹ (~1.5-1.8 g·min⁻¹) provided that multiple transportable carbohydrates are ingested (e.g. 1.2 g·min⁻¹ glucose plus 0.6 g·min⁻¹ of fructose). Surprisingly, small amounts of carbohydrate ingestion during exercise may also enhance the performance of shorter (45-60 min), more intense (>75 % peak oxygen uptake; VO(₂peak)) exercise bouts, despite the fact that endogenous carbohydrate stores are unlikely to be limiting. The mechanism(s) responsible for such ergogenic properties of carbohydrate ingestion during short, more intense exercise bouts has been suggested to reside in the central nervous

  16. Gas-filled separators - An overview

    CERN Document Server

    Leino, M

    2003-01-01

    Gas-filled recoil separators have been used in nuclear physics studies since the early fifties. Most notably, they have found use in the separation of evaporation residues of heavy and very heavy elements from unwanted background. Gas-filled separators, alone or coupled to a detector array, offer an efficient, fast, compact and relatively inexpensive solution for nuclear structure studies. A new application is the use of a gas-filled device as a pre-separator in the study of chemical properties of the heaviest elements. Other uses include systematic study of fusion evaporation cross sections and accelerator mass spectrometry. In this contribution, an overview on gas-filled recoil separators, their characteristics, fields of application and possible future developments is given.

  17. Urbanization and Grain Production Efficiency

    Institute of Scientific and Technical Information of China (English)

    Xiaoyang; LI; Dongge; LIU

    2015-01-01

    Based on DEA-Malmquist method,this paper calculated the integrated technology efficiency of grain production and total factor productivity and analyzed factors influencing the grain production technology efficiency using working documents of panel structure. Research results indicate that grain production integrated technology efficiency of China is relatively low,technology utilization level is low,and it remains at the stage of decreasing returns to scale,and the pure technology efficiency still has space to increase. Total factor productivity is declining and the total factor productivity of many provinces is relatively low. Since the total factor productivity of eastern areas is higher than central and western areas,it is required to strengthen technological support for grain production. The implementation of urbanization is helpful for promoting increase of grain production technology efficiency in central and eastern areas,but it will exert negative influence on western areas.

  18. Autonomous grain combine control system

    Science.gov (United States)

    Hoskinson, Reed L.; Kenney, Kevin L.; Lucas, James R.; Prickel, Marvin A.

    2013-06-25

    A system for controlling a grain combine having a rotor/cylinder, a sieve, a fan, a concave, a feeder, a header, an engine, and a control system. The feeder of the grain combine is engaged and the header is lowered. A separator loss target, engine load target, and a sieve loss target are selected. Grain is harvested with the lowered header passing the grain through the engaged feeder. Separator loss, sieve loss, engine load and ground speed of the grain combine are continuously monitored during the harvesting. If the monitored separator loss exceeds the selected separator loss target, the speed of the rotor/cylinder, the concave setting, the engine load target, or a combination thereof is adjusted. If the monitored sieve loss exceeds the selected sieve loss target, the speed of the fan, the size of the sieve openings, or the engine load target is adjusted.

  19. Coarse Grained Quantum Dynamics

    CERN Document Server

    Agon, Cesar; Kasko, Skyler; Lawrence, Albion

    2014-01-01

    We consider coarse graining a quantum system divided between short distance and long distance degrees of freedom, which are coupled by the Hamiltonian. Observations using purely long distance observables can be described by the reduced density matrix that arises from tracing out the short-distance observables. The dynamics of this density matrix is that of an open quantum system, and is nonlocal in time, on the order of some short time scale. We describe these dynamics in a model system with a simple hierarchy of energy gaps $\\Delta E_{UV} > \\Delta E_{IR}$, in which the coupling between high-and low-energy degrees of freedom is treated to second order in perturbation theory. We then describe the equations of motion under suitable time averaging, reflecting the limited time resolution of actual experiments, and find an expansion of the master equation in powers of $\\Delta E_{IR}/\\Delta E_{UV}$, in which the failure of the system to be Hamiltonian or even Markovian appears at higher orders in this ratio. We com...

  20. Contraction stresses of composite resin filling materials.

    Science.gov (United States)

    Hegdahl, T; Gjerdet, N R

    1977-01-01

    The polymerization shrinkage of composite resin filling materials and the tensile stresses developed when the shrinkage is restrained were measured in an in vitro experiment. This allows an estimation to be made of the forces exerted upon the enamel walls of cavities filled with the resin in the acid etch technique. The results indicate that the stresses acting on the enamel are low compared to the tensile strength of the enamel.

  1. Frankincense tapping reduces the carbohydrate storage of Boswellia trees

    NARCIS (Netherlands)

    Mengistu, T.; Sterck, F.J.; Fetene, M.; Bongers, F.

    2013-01-01

    Carbohydrates fixed by photosynthesis are stored in plant organs in the form of starch or sugars. Starch and sugars sum to the total non-structural carbohydrate pool (TNC) and may serve as intermediate pools between assimilation and utilization. We examined the impact of tapping on TNC concentration

  2. Carbohydrate-responsive gene expression in adipose tissue of rats

    Science.gov (United States)

    Although obesity is often associated with high fat diets, it can develop from a variety of meal patterns. Excessive intake of simple carbohydrates is one consistent eating behavior leading to obesity. However, the impact of over-consumption of diets with high carbohydrate-to-fat ratios (C/F) on body...

  3. The effect of carbohydrates on alpha-amylase activity measurements

    NARCIS (Netherlands)

    Baks, T.; Janssen, A.E.M.; Boom, R.M.

    2006-01-01

    The Ceralpha method can be used for ¿-amylase activity measurements during the hydrolysis of starch at high substrate concentrations (>40 wt.%). However, the results are affected by the carbohydrates present in the samples. The effect of carbohydrates on the Ceralpha ¿-amylase activity measuremen

  4. Genetic Analyses of Soluble Carbohydrate Concentrations in Onion Bulbs

    Science.gov (United States)

    Fructans are the primary soluble carbohydrate in onion (Allium cepa L.) bulbs and show significant correlations with dry weights and pungency. In this research, we estimated the genetic effects and interactions between two chromosome regions associated with higher amounts of soluble carbohydrates i...

  5. Chemical Changes in Carbohydrates Produced by Thermal Processing.

    Science.gov (United States)

    Hoseney, R. Carl

    1984-01-01

    Discusses chemical changes that occur in the carbohydrates found in food products when these products are subjected to thermal processing. Topics considered include browning reactions, starch found in food systems, hydrolysis of carbohydrates, extrusion cooking, processing of cookies and candies, and alterations in gums. (JN)

  6. Using structure to inform carbohydrate binding module function

    NARCIS (Netherlands)

    Abbott, D. Wade; Lammerts van Bueren, Alicia

    2014-01-01

    Generally, non-catalytic carbohydrate binding module (CBM) specificity has been shown to parallel the catalytic activity of the carbohydrate active enzyme (CAZyme) module it is appended to. With the rapid expansion in metagenomic sequence space for the potential discovery of new CBMs in addition to

  7. Dynamic fluctuations of protein-carbohydrate interactions promote protein aggregation.

    Directory of Open Access Journals (Sweden)

    Vladimir Voynov

    Full Text Available Protein-carbohydrate interactions are important for glycoprotein structure and function. Antibodies of the IgG class, with increasing significance as therapeutics, are glycosylated at a conserved site in the constant Fc region. We hypothesized that disruption of protein-carbohydrate interactions in the glycosylated domain of antibodies leads to the exposure of aggregation-prone motifs. Aggregation is one of the main problems in protein-based therapeutics because of immunogenicity concerns and decreased efficacy. To explore the significance of intramolecular interactions between aromatic amino acids and carbohydrates in the IgG glycosylated domain, we utilized computer simulations, fluorescence analysis, and site-directed mutagenesis. We find that the surface exposure of one aromatic amino acid increases due to dynamic fluctuations. Moreover, protein-carbohydrate interactions decrease upon stress, while protein-protein and carbohydrate-carbohydrate interactions increase. Substitution of the carbohydrate-interacting aromatic amino acids with non-aromatic residues leads to a significantly lower stability than wild type, and to compromised binding to Fc receptors. Our results support a mechanism for antibody aggregation via decreased protein-carbohydrate interactions, leading to the exposure of aggregation-prone regions, and to aggregation.

  8. Why use DFT methods in the study of carbohydrates?

    Science.gov (United States)

    The recent advances in density functional theory (DFT) and computer technology allow us to study systems with more than 100 atoms routinely. This makes it feasible to study large carbohydrate molecules via quantum mechanical methods, whereas in the past, studies of carbohydrates were restricted to ...

  9. Influence of dietary carbohydrate level on endocrine status and hepatic carbohydrate metabolism in the marine fish Sparus sarba.

    Science.gov (United States)

    Leung, L Y; Woo, Norman Y S

    2012-04-01

    Silver sea bream, Sparus sarba, were fed two diets of different carbohydrate levels (2 and 20% dextrin) for 4 weeks, and the effects on organ indices, liver composition, serum metabolite and hormone levels and gene expression profile of key enzymes of carbohydrate metabolism in the liver were investigated. By using real-time PCR, mRNA expression levels of carbohydrate metabolic enzymes including glucokinase (GK, glycolysis), glucose-6-phosphatase (G6Pase, gluconeogenesis), glycogen synthase (GS, glycogenesis), glycogen phosphorylase (GP, glycogenolysis) and glucose-6-phosphate dehydrogenase (G6PDH, pentose phosphate pathway) in liver of sea bream have been examined, and it was found that high dietary carbohydrate level increased mRNA level of GK but decreased mRNA levels of G6Pase and GP. However, mRNA levels of GS and G6PDH were not significantly influenced by dietary carbohydrate. Silver sea bream fed high dietary carbohydrate had higher hepatosomatic index (HSI), liver glycogen and protein, but there were no significant changes in gonadosomatic index (GSI), serum glucose and protein level, as well as liver lipid and moisture level. Pituitary growth hormone (GH) and hepatic insulin-like growth factor I (IGF-I) transcript abundance were assayed by real-time PCR, and it was found that both parameters remained unchanged in fish fed different dietary carbohydrate levels. Serum triiodothyronine (T(3)) and thyroxine (T(4)) were not significantly affected by dietary carbohydrate levels, but lower serum cortisol level was found in fish fed high dietary carbohydrate level. These results suggest that silver sea bream is able to adapt to a diet with high carbohydrate content (up to 20% dextrin), the consumption of which would lead to fundamental re-organization of carbohydrate metabolism resulting in hepatic glycogen deposition.

  10. [Carbohydrate absorption and malabsorption (author's transl)].

    Science.gov (United States)

    Caspary, W F

    1977-06-01

    Starch is digested intraluminally by alpha-amylase to maltose, maltotriose, and alpha-limit dextrins. These products, as well as the disaccharides sucrose and lactose, undergo enzymatic hydrolysis to monosaccharides at the brush border surface. The monosaccharides enter the absorbing cell by specific transport mechanisms ("carriers"). Primary carbohydrate (CH) intolerance is characterized by the congenital or acquired absence of individual brush border enzymes or of monosaccharide "carriers" without morphologic abnormalities of the intestinal villus: lactose, sucrose and trehalose intolerance and glucose-galactose malabsorption (brush border diseases). Secondary CH intolerance arises when surface digestion and absorption are reduced due to structural changes of the intestinal mucosa: e.g., decrease or absence of villi with sprue and reduction of the absorbing surface with intestinal resection. Watery diarrhea is the lead symptom. Many drugs delay or interfere with CH absorption. This action may be viewed either as an unwanted side effect or as a welcome therapeutic principle.

  11. Arabinogalactan proteins: focus on carbohydrate active enzymes

    Directory of Open Access Journals (Sweden)

    Eva eKnoch

    2014-06-01

    Full Text Available Arabinogalactan proteins (AGPs are a highly diverse class of cell surface proteoglycans that are commonly found in most plant species. AGPs play important roles in many cellular processes during plant development, such as reproduction, cell proliferation, pattern formation and growth, and in plant-microbe interaction. However, little is known about the molecular mechanisms of their function. Numerous studies using monoclonal antibodies that recognize different AGP glycan epitopes have shown the appearance of a slightly altered AGP glycan in a specific stage of development in plant cells. Therefore, it is anticipated that the biosynthesis and degradation of AGP glycan is tightly regulated during development. Until recently, however, little was known about the enzymes involved in the metabolism of AGP glycans. In this review, we summarize recent discoveries of carbohydrate active enzymes (CAZy; http://www.cazy.org/ involved in the biosynthesis and degradation of AGP glycans, and we discuss the biological role of these enzymes in plant development.

  12. Carbohydrate based materials for gamma radiation shielding

    Science.gov (United States)

    Tabbakh, F.; Babaee, V.; Naghsh-Nezhad, Z.

    2015-05-01

    Due to the limitation in using lead as a shielding material for its toxic properties and limitation in abundance, price or non-flexibility of other commonly used materials, finding new shielding materials and compounds is strongly required. In this conceptual study carbohydrate based compounds were considered as new shielding materials. The simulation of radiation attenuation is performed using MCNP and Geant4 with a good agreement in the results. It is found that, the thickness of 2 mm of the proposed compound may reduce up to 5% and 50% of 1 MeV and 35 keV gamma-rays respectively in comparison with 15% and 100% for the same thickness of lead.

  13. Glycosidases: a key to tailored carbohydrates.

    Science.gov (United States)

    Bojarová, Pavla; Kren, Vladimír

    2009-04-01

    In recent years, carbohydrate-processing enzymes have become the enzymes of choice in many applications thanks to their stereoselectivity and efficiency. This review presents recent developments in glycosidase-catalyzed synthesis via two complementary approaches: the use of wild-type enzymes with engineered substrates, and mutant glycosidases. Genetic engineering has recently produced glucuronyl synthases, an inverting xylosynthase and the first mutant endo-beta-N-acetylglucosaminidase. A thorough selection of enzyme strains and aptly modified substrates have resulted in rare glycostructures, such as N-acetyl-beta-galactosaminuronates, beta1,4-linked mannosides and alpha1,4-linked galactosides. The efficient selection of mutant enzymes is facilitated by high-throughput screening assays involving the co-expression of coupled enzymes or chemical complementation. Selective glycosidase inhibitors and highly specific glycosidases are finding attractive applications in biomedicine, biology and proteomics.

  14. Carbohydrate structure: the rocky road to automation.

    Science.gov (United States)

    Agirre, Jon; Davies, Gideon J; Wilson, Keith S; Cowtan, Kevin D

    2016-12-08

    With the introduction of intuitive graphical software, structural biologists who are not experts in crystallography are now able to build complete protein or nucleic acid models rapidly. In contrast, carbohydrates are in a wholly different situation: scant automation exists, with manual building attempts being sometimes toppled by incorrect dictionaries or refinement problems. Sugars are the most stereochemically complex family of biomolecules and, as pyranose rings, have clear conformational preferences. Despite this, all refinement programs may produce high-energy conformations at medium to low resolution, without any support from the electron density. This problem renders the affected structures unusable in glyco-chemical terms. Bringing structural glycobiology up to 'protein standards' will require a total overhaul of the methodology. Time is of the essence, as the community is steadily increasing the production rate of glycoproteins, and electron cryo-microscopy has just started to image them in precisely that resolution range where crystallographic methods falter most.

  15. Effect of fermentation on the nutrient and antinutrient composition of baobab (adansonia digitata) seeds and rice (oryza sativa) grains.

    Science.gov (United States)

    Nnam, N M; Obiakor, P N

    2003-01-01

    This study is part of an ongoing investigation on the effect of fermentation on chemical and antinutrient compositions of baobab seeds and rice grains. Baobab seeds and rice grains were cleaned and fermented for 24, 48, and 72 h, respectively, by the microflora present in both the seed and the grains. The pH and titratable acidity of the unfermented and fermented samples were determined. The samples were dried and milled into fine flours respectively. Standard assay techniques were used to evaluate the f lours for proximate, mineral, and some antinutrient composition. Fermentation of baobab decreased protein and carbohydrate but increased fat levels. The rice carbohydrate and fat were decreased during fermentation except for the carbohydrate of the 48 and 72 h fermented samples. Protein was only increased in the 24 h fermented rice. Fermentation had varied effects on the mineral concentrations of the baobab seeds and the rice grains. It reduced their antinutrients (phytate and tannins), decreased pH, and increased the titratable acidity of the samples. The biochemical and biological evaluation of the blends of baobab and rice flours as complementary food is in progress in this laboratory.

  16. Carbohydrate deficient transferrin (CDT) in alcoholic cirrhosis: a kinetic study

    DEFF Research Database (Denmark)

    Henriksen, Jens Henrik Sahl; Grønbaek, M; Møller, Søren

    1997-01-01

    concentration than controls with a low alcohol intake (detected between carbohydrate deficient transferrin in artery and liver vein or artery and renal vein, either in patients with alcoholic cirrhosis (n = 11) or in controls (n = 8......BACKGROUND/AIMS: Carbohydrate deficient transferrin has been introduced as a marker of excessive alcohol intake. The present study was undertaken in order to measure the circulating level of carbohydrate deficient transferrin in patients with alcoholic cirrhosis and to assess arteriovenous kinetics...... of carbohydrate deficient transferrin in liver and kidney. METHODS/RESULTS: The median value of serum carbohydrate deficient transferrin was 16.0 U/l in patients with alcoholic cirrhosis (n = 41), and this value was not significantly different from that of a normal control group (median 17.4 U/l, n = 55, ns...

  17. Carbohydrate CuAAC click chemistry for therapy and diagnosis.

    Science.gov (United States)

    He, Xiao-Peng; Zeng, Ya-Li; Zang, Yi; Li, Jia; Field, Robert A; Chen, Guo-Rong

    2016-06-24

    Carbohydrates are important as signaling molecules and for cellular recognition events, therefore offering scope for the development of carbohydrate-mimetic diagnostics and drug candidates. As a consequence, the construction of carbohydrate-based bioactive compounds and sensors has become an active research area. While the advent of click chemistry has greatly accelerated the progress of medicinal chemistry and chemical biology, recent literature has seen an extensive use of such approaches to construct functionally diverse carbohydrate derivatives. Here we summarize some of the progress, covering the period 2010 to mid-2015, in Cu(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition CuAAC "click chemistry" of carbohydrate derivatives, in the context of potential therapeutic and diagnostic tool development.

  18. Defined presentation of carbohydrates on a duplex DNA scaffold.

    Science.gov (United States)

    Schlegel, Mark K; Hütter, Julia; Eriksson, Magdalena; Lepenies, Bernd; Seeberger, Peter H

    2011-12-16

    A new method for the spatially defined alignment of carbohydrates on a duplex DNA scaffold is presented. The use of an N-hydroxysuccinimide (NHS)-ester phosphoramidite along with carbohydrates containing an alkylamine linker allows for on-column labeling during solid-phase oligonucleotide synthesis. This modification method during solid-phase synthesis only requires the use of minimal amounts of complex carbohydrates. The covalently attached carbohydrates are presented in the major groove of the B-form duplex DNA as potential substrates for murine type II C-type lectin receptors mMGL1 and mMGL2. CD spectroscopy and thermal melting revealed only minimal disturbance of the overall helical structure. Surface plasmon resonance and cellular uptake studies with bone-marrow-derived dendritic cells were used to assess the capability of these carbohydrate-modified duplexes to bind to mMGL receptors.

  19. Methods for Shortening and Extending the Carbon Chain in Carbohydrates

    DEFF Research Database (Denmark)

    Monrad, Rune Nygaard

    2008-01-01

    Carbohydrates play a central role in a variety of physiological and pathological processes such as HIV, cancer and diabetes. The understanding of these processes and the development of specific therapeutic agents is relying on the ability to chemically synthesize unnatural sugars, glycoconjugates...... and carbohydrate mimetics. Such polyhydroxylated compounds are conveniently synthesized from carbohydrates, however, due to the scarcity of many sugars from nature, efficient methods for transformation of readily available carbohydrates into valuable chiral building blocks are required. The work presented...... in this thesis focuses on the development and application of transition metal mediated methods for shortening and extending the carbon chain in carbohydrates thereby providing access to lower and higher sugars.A new catalytic procedure for shortening unprotected sugars by one carbon atom has been developed...

  20. Using Carbohydrate Interaction Assays to Reveal Novel Binding Sites in Carbohydrate Active Enzymes

    DEFF Research Database (Denmark)

    Cockburn, Darrell; Wilkens, Casper; Dilokpimol, Adiphol

    2016-01-01

    Carbohydrate active enzymes often contain auxiliary binding sites located either on independent domains termed carbohydrate binding modules (CBMs) or as so-called surface binding sites (SBSs) on the catalytic module at a certain distance from the active site. The SBSs are usually critical...... for the activity of their cognate enzyme, though they are not readily detected in the sequence of a protein, but normally require a crystal structure of a complex for their identification. A variety of methods, including affinity electrophoresis (AE), insoluble polysaccharide pulldown (IPP) and surface plasmon...... sites, but also for identifying new ones, even without structural data available. We further verify the chosen assays discriminate between known SBS/CBM containing enzymes and negative controls. Altogether 35 enzymes are screened for the presence of SBSs or CBMs and several novel binding sites...

  1. Dietary non-digestible carbohydrates and the resistance to intestinal infections

    NARCIS (Netherlands)

    Bruggencate, ten S.J.M.

    2004-01-01

    Keywords: Non-digestible carbohydrates, prebiotics, inulin, FOS, calcium, microflora, short-chain fatty acids, mucin, intestinal permeability, salmonella, infection, rat, humanDietary non-digestible carbohydrates and the resistance to intestinal infectionsNon-digestible carbohydrates (NDC) stimulate

  2. Carbohydrate-enriched cyanobacterial biomass as feedstock for bio-methane production through anaerobic digestion

    DEFF Research Database (Denmark)

    Markou, Giorgos; Angelidaki, Irini; Georgakakis, Dimitris

    2013-01-01

    The anaerobic digestion performance using carbohydrate-enriched biomass of Arthrospira platensis was studied. The carbohydrate enrichment was achieved after the cultivation of A. platensis under phosphorus limitation conditions. Three biomass compositions (60%, 40% and 20% carbohydrates content) ...

  3. The case for low carbohydrate diets in diabetes management

    Directory of Open Access Journals (Sweden)

    McFarlane Samy I

    2005-07-01

    Full Text Available Abstract A low fat, high carbohydrate diet in combination with regular exercise is the traditional recommendation for treating diabetes. Compliance with these lifestyle modifications is less than satisfactory, however, and a high carbohydrate diet raises postprandial plasma glucose and insulin secretion, thereby increasing risk of CVD, hypertension, dyslipidemia, obesity and diabetes. Moreover, the current epidemic of diabetes and obesity has been, over the past three decades, accompanied by a significant decrease in fat consumption and an increase in carbohydrate consumption. This apparent failure of the traditional diet, from a public health point of view, indicates that alternative dietary approaches are needed. Because carbohydrate is the major secretagogue of insulin, some form of carbohydrate restriction is a prima facie candidate for dietary control of diabetes. Evidence from various randomized controlled trials in recent years has convinced us that such diets are safe and effective, at least in short-term. These data show low carbohydrate diets to be comparable or better than traditional low fat high carbohydrate diets for weight reduction, improvement in the dyslipidemia of diabetes and metabolic syndrome as well as control of blood pressure, postprandial glycemia and insulin secretion. Furthermore, the ability of low carbohydrate diets to reduce triglycerides and to increase HDL is of particular importance. Resistance to such strategies has been due, in part, to equating it with the popular Atkins diet. However, there are many variations and room for individual physician planning. Some form of low carbohydrate diet, in combination with exercise, is a viable option for patients with diabetes. However, the extreme reduction of carbohydrate of popular diets (

  4. Blood Triglycerides Levels and Dietary Carbohydrate Indices in Healthy Koreans

    Science.gov (United States)

    Kang, Ji Yeon

    2016-01-01

    Objectives: Previous studies have obtained conflicting findings regarding possible associations between indices measuring carbohydrate intake and dyslipidemia, which is an established risk factor of coronary heart disease. In the present study, we examined cross-sectional associations between carbohydrate indices, including the dietary glycemic index (GI), glycemic load (GL), total amount of carbohydrates, and the percentage of energy from carbohydrates, and a range of blood lipid parameters. Methods: This study included 1530 participants (554 men and 976 women) from 246 families within the Healthy Twin Study. We analyzed the associations using a generalized linear mixed model to control for familial relationships. Results: Levels of the Apo B were inversely associated with dietary GI, GL, and the amount of carbohydrate intake for men, but these relationships were not significant when fat-adjusted values of the carbohydrate indices were used. Triglyceride levels were positively associated with dietary GI and GL in women, and this pattern was more notable in overweight participants (body mass index [BMI] ≥25 kg/m2). However, total, low-density lipoprotein and high-density lipoprotein cholesterol levels were not significantly related with carbohydrate intake overall. Conclusions: Of the blood lipid parameters we investigated, only triglyceride levels were positively related with dietary carbohydrate indices among women participants in the Healthy Twin Study, with an interactive role observed for BMI. However, these associations were not observed in men, suggesting that the association between blood lipid levels and carbohydrate intake depends on the type of lipid, specific carbohydrate indices, gender, and BMI. PMID:27255074

  5. Comparative analysis of carbohydrate active enzymes in Clostridium termitidis CT1112 reveals complex carbohydrate degradation ability.

    Directory of Open Access Journals (Sweden)

    Riffat I Munir

    Full Text Available Clostridium termitidis strain CT1112 is an anaerobic, gram positive, mesophilic, cellulolytic bacillus isolated from the gut of the wood-feeding termite, Nasutitermes lujae. It produces biofuels such as hydrogen and ethanol from cellulose, cellobiose, xylan, xylose, glucose, and other sugars, and therefore could be used for biofuel production from biomass through consolidated bioprocessing. The first step in the production of biofuel from biomass by microorganisms is the hydrolysis of complex carbohydrates present in biomass. This is achieved through the presence of a repertoire of secreted or complexed carbohydrate active enzymes (CAZymes, sometimes organized in an extracellular organelle called cellulosome. To assess the ability and understand the mechanism of polysaccharide hydrolysis in C. termitidis, the recently sequenced strain CT1112 of C. termitidis was analyzed for both CAZymes and cellulosomal components, and compared to other cellulolytic bacteria. A total of 355 CAZyme sequences were identified in C. termitidis, significantly higher than other Clostridial species. Of these, high numbers of glycoside hydrolases (199 and carbohydrate binding modules (95 were identified. The presence of a variety of CAZymes involved with polysaccharide utilization/degradation ability suggests hydrolysis potential for a wide range of polysaccharides. In addition, dockerin-bearing enzymes, cohesion domains and a cellulosomal gene cluster were identified, indicating the presence of potential cellulosome assembly.

  6. Comparative analysis of carbohydrate active enzymes in Clostridium termitidis CT1112 reveals complex carbohydrate degradation ability.

    Science.gov (United States)

    Munir, Riffat I; Schellenberg, John; Henrissat, Bernard; Verbeke, Tobin J; Sparling, Richard; Levin, David B

    2014-01-01

    Clostridium termitidis strain CT1112 is an anaerobic, gram positive, mesophilic, cellulolytic bacillus isolated from the gut of the wood-feeding termite, Nasutitermes lujae. It produces biofuels such as hydrogen and ethanol from cellulose, cellobiose, xylan, xylose, glucose, and other sugars, and therefore could be used for biofuel production from biomass through consolidated bioprocessing. The first step in the production of biofuel from biomass by microorganisms is the hydrolysis of complex carbohydrates present in biomass. This is achieved through the presence of a repertoire of secreted or complexed carbohydrate active enzymes (CAZymes), sometimes organized in an extracellular organelle called cellulosome. To assess the ability and understand the mechanism of polysaccharide hydrolysis in C. termitidis, the recently sequenced strain CT1112 of C. termitidis was analyzed for both CAZymes and cellulosomal components, and compared to other cellulolytic bacteria. A total of 355 CAZyme sequences were identified in C. termitidis, significantly higher than other Clostridial species. Of these, high numbers of glycoside hydrolases (199) and carbohydrate binding modules (95) were identified. The presence of a variety of CAZymes involved with polysaccharide utilization/degradation ability suggests hydrolysis potential for a wide range of polysaccharides. In addition, dockerin-bearing enzymes, cohesion domains and a cellulosomal gene cluster were identified, indicating the presence of potential cellulosome assembly.

  7. [Performance enhancement by carbohydrate intake during sport: effects of carbohydrates during and after high-intensity exercise].

    Science.gov (United States)

    Beelen, Milou; Cermak, Naomi M; van Loon, Luc J C

    2015-01-01

    Endogenous carbohydrate availability does not provide sufficient energy for prolonged moderate to high-intensity exercise. Carbohydrate ingestion during high-intensity exercise can therefore enhance performance.- For exercise lasting 1 to 2.5 hours, athletes are advised to ingest 30-60 g of carbohydrates per hour.- Well-trained endurance athletes competing for longer than 2.5 hours at high intensity can metabolise up to 90 g of carbohydrates per hour, provided that a mixture of glucose and fructose is ingested.- Athletes participating in intermittent or team sports are advised to follow the same strategies but the timing of carbohydrate intake depends on the type of sport.- If top performance is required again within 24 hours after strenuous exercise, the advice is to supplement endogenous carbohydrate supplies quickly within the first few hours post-exercise by ingesting large amounts of carbohydrate (1.2 g/kg/h) or a lower amount of carbohydrate (0.8 g/kg/h) with a small amount of protein (0.2-0.4 g/kg/h).

  8. Carbohydrate Content in the GDM Diet: Two Views: View 1: Nutrition Therapy in Gestational Diabetes: The Case for Complex Carbohydrates.

    Science.gov (United States)

    Hernandez, Teri L

    2016-05-01

    IN BRIEF Restriction of dietary carbohydrate has been the cornerstone for treatment of gestational diabetes mellitus (GDM). However, there is evidence that a balanced liberalization of complex carbohydrate as part of an overall eating plan in GDM meets treatment goals and may mitigate maternal adipose tissue insulin resistance, both of which may promote optimal metabolic outcomes for mother and offspring.

  9. High mature grain phytase activity in the Triticeae has evolved by duplication followed by neofunctionalization of the purple acid phosphatase phytase (PAPhy) gene

    DEFF Research Database (Denmark)

    Madsen, Claus Krogh; Dionisio, Giuseppe; Holme, Inger

    2013-01-01

    The phytase activity in food and feedstuffs is an important nutritional parameter. Members of the Triticeae tribe accumulate purple acid phosphatase phytases (PAPhy) during grain filling. This accumulation elevates mature grain phytase activities (MGPA) up to levels between ~650 FTU/kg for barley...

  10. Quantitative characterisation of sedimentary grains

    Science.gov (United States)

    Tunwal, Mohit; Mulchrone, Kieran F.; Meere, Patrick A.

    2016-04-01

    Analysis of sedimentary texture helps in determining the formation, transportation and deposition processes of sedimentary rocks. Grain size analysis is traditionally quantitative, whereas grain shape analysis is largely qualitative. A semi-automated approach to quantitatively analyse shape and size of sand sized sedimentary grains is presented. Grain boundaries are manually traced from thin section microphotographs in the case of lithified samples and are automatically identified in the case of loose sediments. Shape and size paramters can then be estimated using a software package written on the Mathematica platform. While automated methodology already exists for loose sediment analysis, the available techniques for the case of lithified samples are limited to cases of high definition thin section microphotographs showing clear contrast between framework grains and matrix. Along with the size of grain, shape parameters such as roundness, angularity, circularity, irregularity and fractal dimension are measured. A new grain shape parameter developed using Fourier descriptors has also been developed. To test this new approach theoretical examples were analysed and produce high quality results supporting the accuracy of the algorithm. Furthermore sandstone samples from known aeolian and fluvial environments from the Dingle Basin, County Kerry, Ireland were collected and analysed. Modern loose sediments from glacial till from County Cork, Ireland and aeolian sediments from Rajasthan, India have also been collected and analysed. A graphical summary of the data is presented and allows for quantitative distinction between samples extracted from different sedimentary environments.

  11. Effects of concentrated arabinoxylan and β-glucan compared with refined wheat and whole grain rye on glucose and appetite in subjects with the metabolic syndrome

    DEFF Research Database (Denmark)

    Hartvigsen, M L; Gregersen, S; Lærke, H N;

    2014-01-01

    grain on glucose, hormone responses and appetite in subjects with the metabolic syndrome (MetS). SUBJECTS/METHODS: Fifteen subjects with MetS participated in this acute, randomised, cross-over intervention study. The test breads provided 50 g of digestible carbohydrate: wheat bread with concentrated...

  12. Intake of whole-grain and fiber-rich rye bread versus refined wheat bread does not differentiate intestinal microbiota composition in Finnish adults with metabolic syndrome

    NARCIS (Netherlands)

    Lappi, J.; Salojärvi, J.; Kolehmainen, M.; Mykkänen, H.; Poutanen, K.; Vos, de W.M.; Salonen, A.

    2013-01-01

    Whole-grain (WG) foods rich in indigestible carbohydrates are thought to modulate the composition of the intestinal microbiota. We investigated in a randomized, parallel, 2-arm 12-wk intervention whether consumption of WG and fiber-rich rye breads compared with refined wheat breads affected the micr

  13. Products deriving from microbial fermentation are linked to insulinaemic response in pigs fed breads prepared from whole-wheat grain and wheat and rye ingredients

    DEFF Research Database (Denmark)

    Theil, Peter Kappel; Jørgensen, Henry Johs. Høgh; Serena, Anja;

    2011-01-01

    The effects of wheat and rye breads made from whole-wheat grain (WWG), wheat aleurone flour (WAF) or rye aleurone flour (RAF) on net portal absorption of carbohydrate-derived nutrients (glucose, SCFA and lactate) and apparent insulin secretion were studied in a model experiment with catheterised...

  14. An Improved Implementation of Grain

    CERN Document Server

    Mansouri, Shohreh Sharif

    2009-01-01

    A common approach to protect confidential information is to use a stream cipher which combines plain text bits with a pseudo-random bit sequence. Among the existing stream ciphers, Non-Linear Feedback Shift Register (NLFSR)-based ones provide the best trade-off between cryptographic security and hardware efficiency. In this paper, we show how to further improve the hardware efficiency of Grain stream cipher. By transforming the NLFSR of Grain from its original Fibonacci configuration to the Galois configuration and by introducing a clock division block, we double the throughput of the 80 and 128-bit key 1bit/cycle architectures of Grain with no area penalty.

  15. Involvement of cross-reactive carbohydrate determinants-specific IgE in pollen allergy testing

    Science.gov (United States)

    Yoshitake, Hiroshi; Matsumoto, Yuma; Kawada, Michitsugu; Takato, Yoshiki; Shinagawa, Kiyomi; Sakurai, Hiroyuki; Saito, Koichiro

    2017-01-01

    Background Specific IgE antibodies against the low-molecular-weight carbohydrate antigen that does not bridge IgE molecules on mast cells are not associated with clinical symptoms. Cross reactivity can be determined in allergen-specific IgE detection assays when the carbohydrate structures between pollen allergens and plant derived food allergens are similar; in such cases, false positive results for grain or legume allergens can be reported for pollen allergic patients who are not sensitized to those allergens. This phenomenon arises owing to the presence of cross-reactive carbohydrate determinants (CCDs). Objective This study aimed to assess the impact of CCD interference on the results for pollen allergen-specific IgE antibodies in the general adult population and to perform CCD inhibition tests evaluating the involvement of CCD on samples positive to pollen allergens. Methods Serum samples from 322 subjects were tested for IgE antibodies to pollens and CCD. The research subjects were given questionnaires about pollen allergic symptoms to help assess the presence of allergies. Allergen IgE antibodies for Japanese cedar, Japanese cypress, orchard grass, ragweed, MUXF, bromelain, horseradish peroxidase (HRP), and ascorbate oxidase (ASOD) were analyzed. Results It was observed that among individuals who tested positive to any of the pollen allergens, the positive ratio of CCD-specific IgE antibody was the highest for HRP (13.5%–50.0%). The results from the inhibition tests revealed that CCD was marginally present. Although IgE antibodies for cedar pollen did not react with CCD, IgE antibodies for Japanese cypress, orchard grass, and ragweed might be detected by the presence of CCD. Conclusion The results of the inhibition tests revealed the obvious presence of CCD suggesting its involvement. Considering these findings, careful evaluation of patient IgE results should be performed for Japanese cypress, orchard grass, and ragweed. PMID:28154803

  16. Early diagenesis of carbohydrates and lignin in mangrove sediments subject to variable redox conditions (French Guiana)

    Science.gov (United States)

    Marchand, C.; Disnar, J. R.; Lallier-Vergès, E.; Lottier, N.

    2005-01-01

    A comparative study of lignin and neutral carbohydrate compositions, combined with C, N and δ 13C analyses, was carried out on sedimentary cores, and on various vascular plant species collected in mangrove swamps of French Guiana. The main purpose of this study was to assess the diagenesis of carbohydrates and lignin in brackish to hypersaline fine-grained mangrove sediments characterized by great changes in redox conditions. Distribution of carbohydrates in sediments reflects both the lability of these compounds and their efficient recycling. They are subject to selective degradation, cellulosic glucose and xylose appearing to be the two most labile neutral sugars. In contrast a relative increase in arabinose, rhamnose, fucose and hemicellulosic glucose between plants and sediments, suggests that they may be more refractory and/or that they also derive from microbial synthesis. The total carbon from lignin-derived phenols is higher in sediments than in mangrove plants as a consequence of their rather refractory character. Nevertheless, evidence of lignin decomposition was found to be independent of local environmental conditions. The various redox processes that occur in mangrove sediments depend on plant species, stages in forest development and season. Different redox conditions induce different mechanisms for the decomposition of lignin and thus induce changes in phenol distributions. At depth, in most mangroves, an increase in (Ad/Al) v ratios and in deoxy sugars (fucose and rhamnose) content was significantly correlated with increased proportions of oxidized allochthonous organic debris deriving from the Amazonian detrital discharge, thus suggesting a specific source effect rather than a diagenesis induced change. Therefore, this study illustrates that both lignin and cellulose, derived from vascular plant debris, can be degraded in waterlogged mangrove sediments, and that their distribution depends on environmental conditions.

  17. Saturated Fats Versus Polyunsaturated Fats Versus Carbohydrates for Cardiovascular Disease Prevention and Treatment.

    Science.gov (United States)

    Siri-Tarino, Patty W; Chiu, Sally; Bergeron, Nathalie; Krauss, Ronald M

    2015-01-01

    The effects of saturated fatty acids (SFAs) on cardiovascular disease (CVD) risk are modulated by the nutrients that replace them and their food matrices. Replacement of SFAs with polyunsaturated fatty acids has been associated with reduced CVD risk, although there is heterogeneity in both fatty acid categories. In contrast, replacement of SFAs with carbohydrates, particularly sugar, has been associated with no improvement or even a worsening of CVD risk, at least in part through effects on atherogenic dyslipidemia, a cluster of traits including small, dense low-density lipoprotein particles. The effects of dietary SFAs on insulin sensitivity, inflammation, vascular function, and thrombosis are less clear. There is growing evidence that SFAs in the context of dairy foods, particularly fermented dairy products, have neutral or inverse associations with CVD. Overall dietary patterns emphasizing vegetables, fish, nuts, and whole versus processed grains form the basis of heart-healthy eating and should supersede a focus on macronutrient composition.

  18. Filled and glycosylated carbon nanotubes for in vivo radioemitter localization and imaging

    Science.gov (United States)

    Hong, Sung You; Tobias, Gerard; Al-Jamal, Khuloud T.; Ballesteros, Belén; Ali-Boucetta, Hanene; Lozano-Perez, Sergio; Nellist, Peter D.; Sim, Robert B.; Finucane, Ciara; Mather, Stephen J.; Green, Malcolm L. H.; Kostarelos, Kostas; Davis, Benjamin G.

    2010-06-01

    Functionalization of nanomaterials for precise biomedical function is an emerging trend in nanotechnology. Carbon nanotubes are attractive as multifunctional carrier systems because payload can be encapsulated in internal space whilst outer surfaces can be chemically modified. Yet, despite potential as drug delivery systems and radiotracers, such filled-and-functionalized carbon nanotubes have not been previously investigated in vivo. Here we report covalent functionalization of radionuclide-filled single-walled carbon nanotubes and their use as radioprobes. Metal halides, including Na125I, were sealed inside single-walled carbon nanotubes to create high-density radioemitting crystals and then surfaces of these filled-sealed nanotubes were covalently modified with biantennary carbohydrates, improving dispersibility and biocompatibility. Intravenous administration of Na125I-filled glyco-single-walled carbon nanotubes in mice was tracked in vivo using single-photon emission computed tomography. Specific tissue accumulation (here lung) coupled with high in vivo stability prevented leakage of radionuclide to high-affinity organs (thyroid/stomach) or excretion, and resulted in ultrasensitive imaging and delivery of unprecedented radiodose density. Nanoencapsulation of iodide within single-walled carbon nanotubes enabled its biodistribution to be completely redirected from tissue with innate affinity (thyroid) to lung. Surface functionalization of 125I-filled single-walled carbon nanotubes offers versatility towards modulation of biodistribution of these radioemitting crystals in a manner determined by the capsule that delivers them. We envisage that organ-specific therapeutics and diagnostics can be developed on the basis of the nanocapsule model described here.

  19. Seepage characteristics of collapse column fillings

    Institute of Scientific and Technical Information of China (English)

    Zhang Boyang; Bai Haibo; Zhang Kai

    2016-01-01

    With concealment and hysteresis, water-inrush from Karst collapse column has become an important security hazard of lower group coal mining in North China. Based on the MTS815.02 seepage test system, we analyzed the impact of consolidation pressure, initial moisture content and confining pressure on the permeability of fillings in order to study the seepage characteristics of collapse column fillings. The results show that:(1) The permeability of collapse column fillings is of the order of 10?16–10?15 magni-tude and decreases with an increase in consolidation pressure and decrease in initial moisture content. (2) The essence of filling seepage law change is the change in porosity, and a power function relationship exists between the permeability ratio and porosity ratio. (3) With increasing confining pressure, the per-meability of fillings decreases. However, under low confining pressure (1.2–4 MPa), the change of confin-ing pressure has no obvious influence on the permeability.

  20. INFLUENCE OF FILLING WATER ON AIR CONCENTRATION

    Institute of Scientific and Technical Information of China (English)

    WU Jian-hua; MA Fei; DAI Hui-chao

    2011-01-01

    The filling water inside the cavity below an aerator occurs for the flow of low Froude number or the small bottom slope of a spillway.The aerator may cease to protect against cavitation damages,and may even act as a generator of cavitation if it is fully filled by water.The experiments were conducted to investigate the influences of the geometric parameters,and then the filling water on the air concentration.The results show that the filling water,or the net cavity length,is closely related to the plunging jet length for a given aerator,and the air concentration at some section is proportional to the ratio Ln/Lj at a fixed Lj for different geometric parameters of aerators.Secondly,at the same ratio of Ln / Lj,the aerator with a larger height or a larger angle of ramp,or a larger bottom slope,would have a larger plunging jet length,and then a larger net cavity length based on the ratio of Ln / Lj.As a result,the large space of cavity,or the high air concentration of the flow could be obtained although the filling water increases also based on the fact that Lf=Lj- Ln.It is the space of the cavity that is the dominant factor to affect the air concentration of the flow.

  1. Characterization of carbohydrates in rainwater from the southeastern North Carolina.

    Science.gov (United States)

    Mullaugh, Katherine M; Byrd, Jade N; Avery, G Brooks; Mead, Ralph N; Willey, Joan D; Kieber, Robert J

    2014-07-01

    Carbohydrates have been widely reported in atmospheric aerosols, but have not previously been quantified in rainwater. We have identified and quantified a series of 11 specific compounds including monosaccharides (glucose, fructose, arabinose, galactose and pinitol), disaccharides (sucrose and trehalose), sugar alcohols (arabitol, dulcitol and mannitol) and the anhydrosaccharide levoglucosan. Rainwater analyzed in this study includes 52 distinct precipitation events in Wilmington, NC between June 2011 and October 2012. Our analysis indicates carbohydrates typically contribute carbohydrates reached as high as 5.8 μM, with glucose and sucrose typically being the predominant species. The distribution of carbohydrates exhibited a distinct seasonal pattern, with higher concentrations of most carbohydrates, especially sucrose, in spring and summer, driven primarily by increased biogenic inputs during the growing season. Concentrations of carbohydrates were an order of magnitude higher in storms of terrestrial origin compared to marine events, further supporting a terrestrial biogenic origin of most species. Sequential sampling of Hurricane Irene showed significant quantities of carbohydrates present at the end of the storm when air mass back trajectories traversed over land. The highest level of levoglucosan, a compound associated with biomass burning, was detected in rain with an air mass back trajectory that traveled over a region affected by wildfires. When compared to aerosol concentrations reported by others, the sugar concentrations in rain demonstrate wet deposition is an important removal mechanism of this water-soluble and bioavailable fraction of atmospheric particulate organic matter.

  2. Assessing Bacterial Interactions Using Carbohydrate-Based Microarrays

    Directory of Open Access Journals (Sweden)

    Andrea Flannery

    2015-12-01

    Full Text Available Carbohydrates play a crucial role in host-microorganism interactions and many host glycoconjugates are receptors or co-receptors for microbial binding. Host glycosylation varies with species and location in the body, and this contributes to species specificity and tropism of commensal and pathogenic bacteria. Additionally, bacterial glycosylation is often the first bacterial molecular species encountered and responded to by the host system. Accordingly, characterising and identifying the exact structures involved in these critical interactions is an important priority in deciphering microbial pathogenesis. Carbohydrate-based microarray platforms have been an underused tool for screening bacterial interactions with specific carbohydrate structures, but they are growing in popularity in recent years. In this review, we discuss carbohydrate-based microarrays that have been profiled with whole bacteria, recombinantly expressed adhesins or serum antibodies. Three main types of carbohydrate-based microarray platform are considered; (i conventional carbohydrate or glycan microarrays; (ii whole mucin microarrays; and (iii microarrays constructed from bacterial polysaccharides or their components. Determining the nature of the interactions between bacteria and host can help clarify the molecular mechanisms of carbohydrate-mediated interactions in microbial pathogenesis, infectious disease and host immune response and may lead to new strategies to boost therapeutic treatments.

  3. Linking Bacillus cereus Genotypes and Carbohydrate Utilization Capacity.

    Directory of Open Access Journals (Sweden)

    Alicja K Warda

    Full Text Available We characterised carbohydrate utilisation of 20 newly sequenced Bacillus cereus strains isolated from food products and food processing environments and two laboratory strains, B. cereus ATCC 10987 and B. cereus ATCC 14579. Subsequently, genome sequences of these strains were analysed together with 11 additional B. cereus reference genomes to provide an overview of the different types of carbohydrate transporters and utilization systems found in B. cereus strains. The combined application of API tests, defined growth media experiments and comparative genomics enabled us to link the carbohydrate utilisation capacity of 22 B. cereus strains with their genome content and in some cases to the panC phylogenetic grouping. A core set of carbohydrates including glucose, fructose, maltose, trehalose, N-acetyl-glucosamine, and ribose could be used by all strains, whereas utilisation of other carbohydrates like xylose, galactose, and lactose, and typical host-derived carbohydrates such as fucose, mannose, N-acetyl-galactosamine and inositol is limited to a subset of strains. Finally, the roles of selected carbohydrate transporters and utilisation systems in specific niches such as soil, foods and the human host are discussed.

  4. Export Rebates on Grain Finished

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ According to the State Statistics Bureau, China's consumer price index (CPI) jumped by 6.9 percent year-on-year last November, approaching the high levels seen at the end of 1996, one of the largest areas of increase was the price of grain,which rose by 6.6 percent. There is no doubt the growth in CPI can be mainly attributed to soaring food prices and the diffusion effect of oil price adjustments. Large orders for grain exports have put Chinese grain suppliers under tremendous pressure. In order to solve this problem, the Finance Ministry and State Administration of Taxation together announced that 84 categories of export tax rebates on major types of grain will be eliminated.

  5. Export Rebates on Grain Finished

    Institute of Scientific and Technical Information of China (English)

    Liu; Xinwen

    2008-01-01

      According to the State Statistics Bureau, China's consumer price index (CPI) jumped by 6.9 percent year-on-year last November, approaching the high levels seen at the end of 1996, one of the largest areas of increase was the price of grain,which rose by 6.6 percent. There is no doubt the growth in CPI can be mainly attributed to soaring food prices and the diffusion effect of oil price adjustments. Large orders for grain exports have put Chinese grain suppliers under tremendous pressure. In order to solve this problem, the Finance Ministry and State Administration of Taxation together announced that 84 categories of export tax rebates on major types of grain will be eliminated.……

  6. PARASITE MYCOPOPULATION OF SOYBEAN GRAIN

    Directory of Open Access Journals (Sweden)

    Jasenka Ćosić

    2008-07-01

    Full Text Available Disease appearance on soybean can influence quality and quantity of yield. Different spieces of saprophyte and parasite fungi can be isolated from stems, pods and grain of soybean. The aim of the research was to evaluate the incidence of important disease on natural soybean grain over the period of 4 years (2004-2007 of experiment held on the location Sopot-Vinkovci and included 9 cultivars of soybean. The following plant pathogenic fungi were identified: Peronospora, Sclerotinia, Cercospora, Fusarium and Diaporthe/Phomopsis. The most frequent fungi on soybean grains were: Cladosporium, Alternaria, Penicillium, Aspergillus and Epicoccum. The health condition of the natural soybean grains over the four years period on all cultivars was good.

  7. CO2-induced changes in mineral stoichiometry of wheat grains

    Science.gov (United States)

    Broberg, Malin; Pleijel, Håkan; Högy, Petra

    2016-04-01

    A comprehensive review of experiments with elevated CO2 (eCO2) presenting data on grain mineral concentration in wheat grain was made. Data were collected both from FACE (Free-Air CO2 Enrichment) and OTC (Open-Top Chamber) experiments. Analysis was made i) by deriving response functions for the relative effect on yield and mineral concentration in relation to CO2 concentration, ii) meta-analysis to test the magnitude and significance of observed effects and iii) comparison of the CO2 effect on the accumulation of different minerals in relation to accumulation of biomass and accumulation of N. Data were obtained for the following minerals: N, Zn, Mn, K, Ca, Mg, P, Fe, S, Cr, Cu, Cd and Na. In addition, data for starch, the dominating carbohydrate of wheat grain, were extracted. The responses ranged from near zero effects to strong negative effects of eCO2 on mineral concentration. The order of effect size was the following (from largest to smallest effect) for the different elements: Fe, Ca, S, Zn, Cd, N, Mg, Mn, P, Cu, Cr, K and Na. Particularly strong negative impacts of eCO2 were found in the essential mineral elements Fe, S, Ca, Zn and Mg. Especially Fe, Zn and Mg are nutrients for which deficiency in humans is a problem in todaýs world. The rather large differences in response of different elements indicated that the CO2-induced responses cannot be explained by a simple growth dilution model. Rather, uptake and transport mechanisms may have to be considered in greater detail, as well as the link of different elements with the uptake of nitrogen, the quantitatively dominating mineral nutrient, to explain the observed pattern. No effect of eCO2 on starch concentration could be demonstrated. This substantiates the rejection of a simple dilution model, since one would expect starch concentrations to be elevated in order to explain reduced mineral concentrations by carbohydrate dilution. The concentrations of toxic Cd was negatively affected, in principle a

  8. PARASITE MYCOPOPULATION OF SOYBEAN GRAIN

    OpenAIRE

    Jasenka Ćosić; Karolina Vrandečić; Draženka Jurković; Ivan Ereš; Jelena Poštić

    2008-01-01

    Disease appearance on soybean can influence quality and quantity of yield. Different spieces of saprophyte and parasite fungi can be isolated from stems, pods and grain of soybean. The aim of the research was to evaluate the incidence of important disease on natural soybean grain over the period of 4 years (2004-2007) of experiment held on the location Sopot-Vinkovci and included 9 cultivars of soybean. The following plant pathogenic fungi were identified: Peronospora, Sclerotinia, Cercospora...

  9. Detecting Molecular Features of Spectra Mainly Associated with Structural and Non-Structural Carbohydrates in Co-Products from BioEthanol Production Using DRIFT with Uni- and Multivariate Molecular Spectral Analyses

    Directory of Open Access Journals (Sweden)

    Zhiyuan Niu

    2011-03-01

    Full Text Available The objective of this study was to use DRIFT spectroscopy with uni- and multivariate molecular spectral analyses as a novel approach to detect molecular features of spectra mainly associated with carbohydrate in the co-products (wheat DDGS, corn DDGS, blend DDGS from bioethanol processing in comparison with original feedstock (wheat (Triticum, corn (Zea mays. The carbohydrates related molecular spectral bands included: A_Cell (structural carbohydrates, peaks area region and baseline: ca. 1485–1188 cm−1, A_1240 (structural carbohydrates, peak area centered at ca. 1240 cm−1 with region and baseline: ca. 1292–1198 cm−1, A_CHO (total carbohydrates, peaks region and baseline: ca. 1187–950 cm-1, A_928 (non-structural carbohydrates, peak area centered at ca. 928 cm−1 with region and baseline: ca. 952–910 cm−1, A_860 (non-structural carbohydrates, peak area centered at ca. 860 cm−1 with region and baseline: ca. 880–827 cm-1, H_1415 (structural carbohydrate, peak height centered at ca. 1415 cm−1 with baseline: ca. 1485–1188 cm−1, H_1370 (structural carbohydrate, peak height at ca. 1370 cm−1 with a baseline: ca. 1485–1188 cm−1. The study shows that the grains had lower spectral intensity (KM Unit of the cellulosic compounds of A_1240 (8.5 vs. 36.6, P < 0.05, higher (P < 0.05 intensities of the non-structural carbohydrate of A_928 (17.3 vs. 2.0 and A_860 (20.7 vs. 7.6 than their co-products from bioethanol processing. There were no differences (P > 0.05 in the peak area intensities of A_Cell (structural CHO at 1292–1198 cm−1 and A_CHO (total CHO at 1187–950 cm−1 with average molecular infrared intensity KM unit of 226.8 and 508.1, respectively. There were no differences (P > 0.05 in the peak height intensities of H_1415 and H_1370 (structural CHOs with average intensities 1.35 and 1.15, respectively. The multivariate molecular spectral analyses were able to discriminate and classify between the corn and corn DDGS

  10. M3B: A coarse grain model for the simulation of oligosaccharides and their water mixtures.

    Science.gov (United States)

    Goddard, William A.; Cagin, Tahir; Molinero, Valeria

    2003-03-01

    Water and sugar dynamics in concentrated carbohydrate solutions is of utmost importance in food and pharmaceutical technology. Water diffusion in concentrated sugar mixtures can be slowed down many orders of magnitude with respect to bulk water [1], making extremely expensive the simulation of these systems with atomistic detail for the required time-scales. We present a coarse grain model (M3B) for malto-oligosaccharides and their water mixtures. M3B speeds up molecular dynamics simulations about 500-1000 times with respect to the atomistic model while retaining enough detail to be mapped back to the atomistic structures with low uncertainty in the positions. The former characteristic allows the study of water and carbohydrate dynamics in supercooled and polydisperse mixtures with characteristic time scales above the nanosecond. The latter makes M3B well suited for combined atomistic-mesoscale simulations. We present the parameterization of M3B force field for water and a family of technologically relevant glucose oligosaccharides, the alpha-(1->4) glucans. The coarse grain force field is completely parameterized from atomistic simulations to reproduce the density, cohesive energy and structural parameters of amorphous sugars. We will show that M3B is capable to describe the helical character of the higher oligosaccharides, and that the water structure in low moisture mixtures shows the same features obtained with the atomistic and M3B models. [1] R Parker, SG Ring: Carbohydr. Res. 273 (1995) 147-55.

  11. Form Filling with Self-Compacting Concrete

    DEFF Research Database (Denmark)

    Thrane, Lars Nyholm

    2007-01-01

    a Bingham model with a yield stress and plastic viscosity. In this way the accuracy and ability of CFD to simulate the homogeneous flow on a realistic form filling scale is utilized compared to, e.g. a discrete particle flow approach which requires a much larger computer capacity, especially in three...... to the homogeneous form filling simulation provides a theoretical tool to assess parts of the formwork where there is a risk of blocking. The modelling approach has been applied to selected flow domains comprising standard test methods, the slump flow test and the L-box test, and form filling applications...... nuværende praksis for valg af materialer og støbemetoder er erfaringsbaseret; beregningsbaserede planlægningsmetoder forventes at kunne motivere en forøget anvendelse af SCC. Projektet har haft til formål at etablere modeller til simulering af formfyldning med SCC under hensyntagen til betonens flyde...

  12. Superconductivity of small metal grains

    Institute of Scientific and Technical Information of China (English)

    ZHENG; Renrong; CHEN; Zhiqian; ZHU; Shunquan

    2005-01-01

    The formulas of the energy gap and superconducting critical temperature appropriate for systems with both odd and even number of electrons are derived; the bases of the derivations are BCS theory and energy level statistics. Numerical results qualitatively agree with the experimental phenomena. i.e., the superconductivity of small metallic grains will first enhance then decrease to zero when the grain are getting smaller and smaller. The calculations indicate that the above phenomena happen in the metallic grains belonging to Gaussian Orthogonal Ensemble (GOE) and Gaussian Unitary ensemble (GUE) with zero spin; The superconductivity of small metallic grains in Gaussian Symplectic Ensemble (GSE) will monotonically decrease to zero with the decreasing of the grain size. The analyses suggest that the superconductivity enhancements come from pairing and the balance of the strengths between spin-orbital coupling and external magnetic field. In order to take the latter into account, it is necessary to include the level statistics given by Random Matrix Theory (RMT) in describing small metallic grains.

  13. Hydrogen and methane breath tests for evaluation of resistant carbohydrates

    DEFF Research Database (Denmark)

    Rumessen, J J

    1992-01-01

    This review considers in detail the background, principles, techniques, limitations and advantages of the hydrogen and methane breath tests. Resistant food carbohydrates, defined as dietary carbohydrates partly or totally escaping small intestinal assimilation, are fermented in the human colon...... carbohydrates. Methane breath tests may supplement the information gained from hydrogen measurements, but further evaluations are needed. The hydrogen breath technique is rapid, simple and non-invasive as well as non-radioactive. It may be carried out in a large number of intact individuals under physiological...

  14. Simple mucin-type carbohydrate antigens in major salivary glands

    DEFF Research Database (Denmark)

    Therkildsen, M H; Mandel, U; Thorn, J

    1994-01-01

    -defined monoclonal antibodies (MAb) on frozen and paraffin-embedded normal salivary gland tissue from 22 parotid, 14 submandibular, six sublingual, and 13 labial glands to elucidate the simple mucin-type glycosylation pattern in relation to cyto- and histodifferentiation. The investigated carbohydrate structures......Simple mucin-type carbohydrate antigens Tn, sialosyl-Tn and T are often markers of neoplastic transformation and have very limited expression in normal tissues. We performed an immunohistological study of simple mucin-type carbohydrate antigens, including H and A variants, with well...... antigens indicates that these structures may be of value as markers of salivary gland tumors....

  15. PREPARATION OF CHEMICALLY WELL-DEFINED CARBOHYDRATE DENDRIMER CONJUGATES

    DEFF Research Database (Denmark)

    2004-01-01

    A method for the synthesis of dendrimer conjugates having a well-defined chemical structure, comprising one or more carbohydrate moieties and one or more immunomodulating substances coupled to a dendrimer, is presented. First, the carbohydrate is bound to the dendrimer in a chemoselective manner....... Subsequently, the immunomodulating substance is also bound in a chemoselective manner, to give a dendrimer conjugate with a well-defined structure and connectivity and containing a precise, pre-determined ratio of carbohydrate to immunomodulating substance. The invention also relates to novel dendrimer...

  16. Carbohydrate – protein complex of the waste of climacoptera obtusifolia

    Directory of Open Access Journals (Sweden)

    G. Seitimova

    2013-05-01

    Full Text Available Extract from Climacoptera obtusifolia family Chenopodiaceae has antidiabetic activity. For the first time carbohydrate-protein complex of the waste from Climacoptera obtusifolia was studied. It was found that the quantity of extractive substances with 80% ethanol in aerial part – 52;6% and in the waste – 12;35%. The technique of separation of the carbohydrate-protein complex from the waste from Climacoptera obtusifolia is developed by means of classical and physical-chemical methods. The composition of carbohydrate-protein complex was identified: oligosaccharide; polysaccharide and two glycoproteins.

  17. Carbohydrate metabolism and its regulatory hormones in anorexia nervosa.

    Science.gov (United States)

    Casper, R C

    1996-04-16

    Findings of studies of carbohydrate metabolism in anorexia nervosa are reviewed. Topics covered included fasting blood sugar concentrations; serum insulin concentrations, insulin receptor binding activity, insulin sensitivity, and insulin resistance; plasma ketone bodies and free fatty acids; glucose tolerance tests; growth hormone, cortisol, intestinal hormones, and norepinephrine. Metabolic changes reported in anorexia nervosa are similar to those found in human and animal studies of states of caloric and carbohydrate restriction. Restoration of normal body weight is associated with normalization of virtually all measures. It is concluded that published studies offer no conclusive evidence for a syndrome-specific impairment in carbohydrate metabolism in anorexia nervosa.

  18. Zn uptake, translocation and grain Zn loading in rice (Oryza sativa L.) genotypes selected for Zn deficiency tolerance and high grain Zn.

    Science.gov (United States)

    Impa, Somayanda M; Morete, Mark J; Ismail, Abdelbagi M; Schulin, Rainer; Johnson-Beebout, Sarah E

    2013-07-01

    Zn deficiency is a widespread problem in rice (Oryza sativa L.) grown under flooded conditions, limiting growth and grain Zn accumulation. Genotypes with Zn deficiency tolerance or high grain Zn have been identified in breeding programmes, but little is known about the physiological mechanisms conferring these traits. A protocol was developed for growing rice to maturity in agar nutrient solution (ANS), with optimum Zn-sufficient growth achieved at 1.5 μM ZnSO4.7H2O. The redox potential in ANS showed a decrease from +350 mV to -200 mV, mimicking the reduced conditions of flooded paddy soils. In subsequent experiments, rice genotypes contrasting for Zn deficiency tolerance and grain Zn were grown in ANS with sufficient and deficient Zn to assess differences in root uptake of Zn, root-to-shoot Zn translocation, and in the predominant sources of Zn accumulation in the grain. Zn efficiency of a genotype was highly influenced by root-to-shoot translocation of Zn and total Zn uptake. Translocation of Zn from root to shoot was more limiting at later growth stages than at the vegetative stage. Under Zn-sufficient conditions, continued root uptake during the grain-filling stage was the predominant source of grain Zn loading in rice, whereas, under Zn-deficient conditions, some genotypes demonstrated remobilization of Zn from shoot and root to grain in addition to root uptake. Understanding the mechanisms of grain Zn loading in rice is crucial in selecting high grain Zn donors for target-specific breeding and also to establish fertilizer and water management strategies for achieving high grain Zn.

  19. 30 CFR 816.72 - Disposal of excess spoil: Valley fills/head-of-hollow fills.

    Science.gov (United States)

    2010-07-01

    ..., 6-hour precipitation event. (b) Rock-core chimney drains. A rock-core chimney drain may be used in a... as the fill is not located in an area containing intermittent or perennial streams. A rock-core... upstream drainage is diverted around the fill. The alternative rock-core chimney drain system shall...

  20. Filling defect artefacts in magnetic resonance urography

    Energy Technology Data Exchange (ETDEWEB)

    Girish, G.; Chooi, W.K.; Morcos, S.K. [Department of Diagnostic Imaging, Northern General Hospital, Sheffield Teaching Hospitals NHS Trust, S5 7AU, Sheffield (United Kingdom)

    2004-01-01

    The aim of this study was to determine the prevalence of filling defect artefacts (FDA) in magnetic resonance urography (MRU). Retrospectively, we assessed MRU examinations of 45 patients with neurogenic bladder dysfunction (21 men, 24 women; mean age 35 years, age range 18-71 years). The MRU was performed 30 min after intramuscular injection of 20 mg frusemide using heavily T2-weighted fast-spin-echo techniques [axial, thick coronal slab, coronal maximum intensity projection (MIP) images] with fat saturation. The images were reviewed by two observers to determine the presence of filling defects and dilatation of pelvicalyceal system and ureters. The filling defects were classified into central, eccentric and complete. Clinical course and plain films were reviewed to determine significance of the detected filling defects. True filling defects were observed in 5 patients (11%) and all due to stones seen on the plain radiograph of the abdomen. Filling defects artefacts (FDAs) were seen in 23 patients (51%; 17 pelvicalyceal system, 17 upper third of ureters, 7 mid ureters and 1 distal ureter). No stones were seen on the plain radiograph of these patients and they had a favourable clinical course for over 24 months. The true filling defects were large in size, eccentric in position and seen in more than one sequence of the MRU examination (axial, n=5; slab, n=5; and MIP, n=4). Four (80%) of the patients with true defects and 21 (91%) of those with FDAs had dilatation of the pelvicalyceal system and ureters. The FDAs were small in size, centrally placed (74%) and always seen in axial images, rarely in slab images (2 cases) and not seen in MIP images. Artefactual filling defects can be seen in MRU examinations. The cause of the FDAs is not fully explained and could be secondary to turbulent and fast flow of the urine. Some of the FDAs seen in the calyces could be due to the tips of the papillae. Awareness of such defects obviates misinterpretation and prevents

  1. Creep of granulated loose-fill insulation

    DEFF Research Database (Denmark)

    Rasmussen, Torben Valdbjørn

    This report presents a proposal for a standardised method for creep tests and the necessary theoretical framework that can be used to describe creep of a granulated loose-fill material. Furthermore results from a round robin test are shown. The round robin test was carried out in collaboration...... with SP-Building Physics in Sweden and VTT Building Technology in Finland. For the round robin test a cellulosic fibre insulation material was used. The proposed standardised method for creep tests and theories are limited to cases when the granulated loose-fill material is exposed to a constant...

  2. Experimental study on capillary filling in nanochannels

    Science.gov (United States)

    Yang, Min; Cao, Bing-Yang; Wang, Wei; Yun, He-Ming; Chen, Bao-Ming

    2016-10-01

    We investigated the capillary filling kinetics of deionized water in nanochannels with heights of 50-120 nm. The measured position of the moving meniscus was proportional to the square root of time, as predicted by the LW equation. However, the extracted slopes were significantly smaller than the predictions based on the bulk properties. This unusual behavior was found to be mainly caused by the electro-viscous effect and dynamic contact angle, which was significantly larger than the static angle. In addition, when the filling distance reached about 600 μm, bubbles tended to be formed, leading to the main meniscus was almost immobile.

  3. Singularity links with exotic Stein fillings

    OpenAIRE

    Özbağcı, Burak; Akhmedov, Anar

    2012-01-01

    arXiv:1206.2468v3 [math.GT] 14 May 2014 SINGULARITY LINKS WITH EXOTIC STEIN FILLINGS ANAR AKHMEDOV AND BURAK OZBAGCI ABSTRACT. In [4], it was shown that there exist infinitely many contact Seifert fibered 3-manifolds each of which admits infinitely many exotic (homeomorphic but pairwise non-diffeomorphic) simply-connected Stein fillings. Here we extend this result to a larger set of contact Seifert fibered 3-manifolds with many singular fibers and observe that these 3- ma...

  4. A mathematical model for lake ontogeny in terms of filling with sediments and macrophyte vegetation

    Energy Technology Data Exchange (ETDEWEB)

    Brydsten, Lars [Umeaa Univ. (Sweden). Dept. of Biology and Environmental Science

    2004-05-01

    A mathematical model for simulation of lake basin filling processes in areas with positive shore displacement was constructed. The model was calibrated using sediment and catchments data from eight existing lake basins situated in the northern coastal area of the province of Uppland, Sweden. The lake basin filling processes were separated into three phases: basin filling with wave-washed material (silt, silty sand or sand), filling with fine-grained material during the shallow gulf and lake stages, respectively, and filling with vegetation during the lake stage. The basin filling rates for wave-washed material were generally low but varied considerably both between and within lakes. The mean basin filling rate of wave-washed material was 4.1%. The volume of inorganic sediments produced, and basin filling rates during the shallow gulf and lake phases were determined for all the eight lakes. The relationship between basin filling rate and parameters describing the catchments, the former postglacial basins and the lakes, respectively, was determined using multiple regression analysis. The basin filling rate with inorganic sediments was best described by parameters related to former postglacial basin morphometry and current lake morphometry, e.g. basin volume, lake volume, and lake area. The goodness of fit turned out to be 0.99 for a simple regression with basin volume as the sole independent variable. The basin filling with vegetation (Phragmites australis followed by Sphagnum spp.) was treated as a 2-dimensional process. A dataset with 84 bogs was selected from a digital soil map. The ages of the bogs were calculated using a digital elevation map and an equation for shore displacement. The choke-up rate was then calculated by dividing the area of the bogs with their age. A strong exponential relationship exists between areas of the bogs and choke-up rat, and this relationship was then used in the model. The resulting model starts by filling the former coastal basin

  5. Cationized Carbohydrate Gas-Phase Fragmentation Chemistry

    Science.gov (United States)

    Bythell, Benjamin J.; Abutokaikah, Maha T.; Wagoner, Ashley R.; Guan, Shanshan; Rabus, Jordan M.

    2016-11-01

    We investigate the fragmentation chemistry of cationized carbohydrates using a combination of tandem mass spectrometry, regioselective labeling, and computational methods. Our model system is D-lactose. Barriers to the fundamental glyosidic bond cleavage reactions, neutral loss pathways, and structurally informative cross-ring cleavages are investigated. The most energetically favorable conformations of cationized D-lactose were found to be similar. In agreement with the literature, larger group I cations result in structures with increased cation coordination number which require greater collision energy to dissociate. In contrast with earlier proposals, the B n -Y m fragmentation pathways of both protonated and sodium-cationized analytes proceed via protonation of the glycosidic oxygen with concerted glycosidic bond cleavage. Additionally, for the sodiated congeners our calculations support sodiated 1,6-anhydrogalactose B n ion structures, unlike the preceding literature. This affects the subsequent propensity of formation and prediction of B n /Y m branching ratio. The nature of the anomeric center (α/β) affects the relative energies of these processes, but not the overall ranking. Low-energy cross-ring cleavages are observed for the metal-cationized analytes with a retro-aldol mechanism producing the 0,2 A 2 ion from the sodiated forms. Theory and experiment support the importance of consecutive fragmentation processes, particularly for the protonated congeners at higher collision energies.

  6. Saturated fat, carbohydrates and cardiovascular disease.

    Science.gov (United States)

    Kuipers, R S; de Graaf, D J; Luxwolda, M F; Muskiet, M H A; Dijck-Brouwer, D A J; Muskiet, F A J

    2011-09-01

    The dietary intake of saturated fatty acids (SAFA) is associated with a modest increase in serum total cholesterol, but not with cardiovascular disease (CVD). Replacing dietary SAFA with carbohydrates (CHO), notably those with a high glycaemic index, is associated with an increase in CVD risk in observational cohorts, while replacing SAFA with polyunsaturated fatty acids (PUFA) is associated with reduced CVD risk. However, replacing a combination of SAFA and trans-fatty acids with n-6 PUFA (notably linoleic acid) in controlled trials showed no indication of benefit and a signal toward increased coronary heart disease risk, suggesting that n-3 PUFA may be responsible for the protective association between total PUFA and CVD. High CHO intakes stimulate hepatic SAFA synthesis and conservation of dietary SAFA . Hepatic de novo lipogenesis from CHO is also stimulated during eucaloric dietary substitution of SAFA by CHO with high glycaemic index in normo-insulinaemic subjects and during hypocaloric high-CHO/low-fat diets in subjects with the metabolic syndrome. The accumulation of SAFA stimulates chronic systemic low-grade inflammation through its mimicking of bacterial lipopolysaccharides and÷or the induction of other pro-inflammatory stimuli. The resulting systemic low-grade inflammation promotes insulin resistance, reallocation of energy-rich substrates and atherogenic dyslipidaemia that concertedly give rise to increased CVD risk. We conclude that avoidance of SAFA accumulation by reducing the intake of CHO with high glycaemic index is more effective in the prevention of CVD than reducing SAFA intake per se.

  7. Influence of fines content on the anti-frost properties of coarse-grained soil

    Institute of Scientific and Technical Information of China (English)

    TianLiang Wang; ZuRun Yue; TieCheng Sun; JinChuang Hua

    2015-01-01

    This paper aims to determine the optimal fines content of coarse-grained soil required to simultaneously achieve weaker frost susceptibility and better bearing capacity. We studied the frost susceptibility and strength properties of coarse-grained soil by means of frost heaving tests and static triaxial tests, and the results are as follows: (1) the freezing temperature of coarse-grained soil decreased gradually and then leveled off with incremental increases in the percent content of fines; (2) the fines content proved to be an important factor influencing the frost heave susceptibility and strength properties of coarse-grained soil. With incremental increases in the percent content of fines, the frost heave ratio increased gradually and the cohesion function of fines effectively enhanced the shear strength of coarse-grained soil before freeze-thaw, but the frost susceptibility of fines weakened the shear strength of coarse-grained soil after freeze-thaw; (3) with increasing numbers of freeze-thaw cycles, the shear strength of coarse-grained soil decreased and then stabilized after the ninth freeze-thaw cycle, and therefore the mechanical indexes of the ninth freeze-thaw cycle are recommended for the engi-neering design values; and (4) considering frost susceptibility and strength properties as a whole, the optimal fines content of 5% is recommended for railway subgrade coarse-grained soil fillings in frozen regions.

  8. The structure of filled skutterudites and the local vibration behavior of the filling atom

    Science.gov (United States)

    Zhou, Xiaojuan; Zong, Peng-an; Chen, Xihong; Tao, Juzhou; Lin, He

    2017-02-01

    Both of atomic pair distribution function (PDF) and extended x-ray absorption fine structure (EXAFS) experiments have been carried out on unfilled and Yb-filled skutterudites YbxCo4Sb12 (x=0, 0.15, 0.2 and 0.25) samples. The structure refinements on PDF data confirm the large amplitude vibration of Yb atom and the dependence of Yb vibration amplitude on the filling content. Temperature dependent EXAFS experiment on filled skutterudites have been carried out at Yb LⅢ-edge in order to explore the local vibration behavior of filled atom. EXAFS experiments show that the Einstein temperature of the filled atom is very low (70.9 K) which agrees with the rattling behavior.

  9. Dietary substitutions for refined carbohydrate that show promise for reducing risk of type 2 diabetes in men and women.

    Science.gov (United States)

    Maki, Kevin C; Phillips, Alyssa K

    2015-01-01

    Both genetics and lifestyle contribute to type 2 diabetes (T2D), a condition of elevated circulating glucose induced by a collection of metabolic defects including peripheral insulin resistance, elevated hepatic glucose output, and impaired pancreatic insulin secretion. Because the prevalence of T2D and its modifiable risk factors (overweight/obesity, dyslipidemia, hypertension, and physical inactivity) have been increasing in recent decades, there has been growing interest in lifestyle interventions that target T2D management and prevention. Although it is increasingly recognized that lifestyle interventions aimed at encouraging physical activity and reducing body weight can improve insulin sensitivity, nutritional contributions to T2D risk reduction are less clear. Evidence from prospective cohort and randomized controlled trials suggests that diets rich in refined dietary carbohydrate [particularly those with a high glycemic index (GI)] may elevate T2D risk; however, the appropriate combination of macronutrients to optimize metabolic health has not been fully described. To date, the collective evidence suggests that diets rich in low-GI carbohydrates, cereal fiber, resistant starch, fat from vegetable sources (unsaturated fat), and lean sources of protein should be emphasized, whereas refined sugars and grains (high-GI carbohydrates) are to be avoided in order to lower risk of T2D and its related risk factors and comorbidities.

  10. Carbohydrate biomarkers for future disease detection and treatment

    Institute of Scientific and Technical Information of China (English)

    REID; Suazette

    2010-01-01

    Carbohydrates are considered as one of the most important classes of biomarkers for cell types,disease states,protein functions,and developmental states.Carbohydrate"binders"that can specifically recognize a carbohydrate biomarker can be used for developing novel types of site specific delivery methods and imaging agents.In this review,we present selected examples of important carbohydrate biomarkers and how they can be targeted for the development of therapeutic and diagnostic agents.Examples are arranged based on disease categories including(1) infectious diseases,(2) cancer,(3) inflammation and immune responses,(4) signal transduction,(5) stem cell transformation,(6) embryo development,and(7) cardiovascular diseases,though some issues cross therapeutic boundaries.

  11. Nonstructural carbohydrates and return bloom potential differ among cranberry cultivars

    Science.gov (United States)

    explain low fruit set and biennial bearing tendencies of cranberry (Vaccinium macrocarpon). Yet, comparisons of nonstructural carbohydrate concentrations during critical phenological stages across cultivars that differ in biennial bearing tendencies and return bloom potential are lacking, particular...

  12. Registration of Common Wheat Germplasm with Mutations in SBEII Genes Conferring Increased Grain Amylose and Resistant Starch Content

    OpenAIRE

    2016-01-01

    Starch present in the endosperm of common wheat (Triticum aestivum L.) grains is an important source of carbohydrates worldwide. Starches with a greater proportion of amylose have increased levels of resistant starch, a dietary fiber that can provide human health benefits. Induced mutations in STARCH BRANCHING ENZYME II (SBEII) genes in wheat are associated with increased amylose and resistant starch. Ethyl methane sulfonate mutations in SBEIIa and SBEIIb paralogs were combined in the hexaplo...

  13. The Chemistry of Modern Dental Filling Materials.

    Science.gov (United States)

    Nicholson, John W.; Anstice, H. Mary

    1999-01-01

    Discusses materials used by dentists to restore teeth after decay has been removed. Shows how dental-material science is an interdisciplinary field in which chemistry plays a major part. Reviews the many developments polymer chemistry has contributed to the field of dental fillings. (CCM)

  14. Electroviscous effects in capillary filling of nanochannels

    DEFF Research Database (Denmark)

    Mortensen, Asger; Kristensen, Anders

    2008-01-01

    We theoretically examine the widespread hypothesis of an electroviscous origin of the increase in apparent viscosity observed in recent experiments on capillary filling of nanochannels. Including Debye-layer corrections to the hydraulic resistance, we find that the apparent viscosity reaches a ma...

  15. Modelling of the Manifold Filling Dynamics

    DEFF Research Database (Denmark)

    Hendricks, Elbert; Chevalier, Alain Marie Roger; Jensen, Michael

    1996-01-01

    Mean Value Engine Models (MVEMs) are dynamic models which describe dynamic engine variable (or state) responses on time scales slightly longer than an engine event. This paper describes a new model of the intake manifold filling dynamics which is simple and easy to calibrate for use in engine con...

  16. Topological scaling and gap filling at crisis

    Energy Technology Data Exchange (ETDEWEB)

    Szabo, K. Gabor [Department of Mathematics, University of Kansas, Lawrence, Kansas 66045 (United States); Institute for Theoretical Physics, Eoetvoes University, P.O. Box 32, Budapest, H-1518, (Hungary); Lai, Ying-Cheng [Department of Mathematics and Department of Electrical Engineering, Arizona State University, Tempe, Arizona 85287 (United States); Tel, Tamas [Institute for Theoretical Physics, Eoetvoes University, P.O. Box 32, Budapest, H-1518, (Hungary); Grebogi, Celso [Institute for Plasma Research, Department of Mathematics, and Institute for Physical Science and Technology, University of Maryland, College Park, Maryland 20742 (United States)

    2000-05-01

    Scaling laws associated with an interior crisis of chaotic dynamical systems are studied. We argue that open gaps of the chaotic set become densely filled at the crisis due to the sudden appearance of unstable periodic orbits with extremely long periods. We formulate a scaling theory for the associated growth of the topological entropy. (c) 2000 The American Physical Society.

  17. Safety distances for hydrogen filling stations

    NARCIS (Netherlands)

    Matthijsen, A J C M; Kooi, E S

    2006-01-01

    In the context of spatial planning the Dutch Ministry of Housing, Spatial Planning and the Environment asked the Centre for External Safety of the National Institute for Public Health and the Environment (RIVM) to advice on safe distances pertaining to hydrogen filling stations. The RIVM made use of

  18. Irregularly Shaped Space-Filling Truncated Octahedra

    Science.gov (United States)

    Hanson, John Robert

    2008-01-01

    For any parent tetrahedron ABCD, centroids of selected sub-tetrahedra form the vertices of an irregularly shaped space-filling truncated octahedron. To reflect these properties, such a figure will be called an ISTO. Each edge of the ISTO is parallel to and one-eighth the length of one of the edges of tetrahedron ABCD and the volume of the ISTO is…

  19. Sensitive carbohydrate detection using surface enhanced Raman tagging.

    Science.gov (United States)

    Vangala, Karthikeshwar; Yanney, Michael; Hsiao, Cheng-Te; Wu, Wells W; Shen, Rong-Fong; Zou, Sige; Sygula, Andrzej; Zhang, Dongmao

    2010-12-15

    Glycomic analysis is an increasingly important field in biological and biomedical research as glycosylation is one of the most important protein post-translational modifications. We have developed a new technique to detect carbohydrates using surface enhanced Raman spectroscopy (SERS) by designing and applying a Rhodamine B derivative as the SERS tag. Using a reductive amination reaction, the Rhodamine-based tag (RT) was successfully conjugated to three model carbohydrates (glucose, lactose, and glucuronic acid). SERS detection limits obtained with a 633 nm HeNe laser were ∼1 nM in concentration for all the RT-carbohydrate conjugates and ∼10 fmol in total sample consumption. The dynamic range of the SERS method is about 4 orders of magnitude, spanning from 1 nM to 5 μM. Ratiometric SERS quantification using isotope-substituted SERS internal references allows comparative quantifications of carbohydrates labeled with RT and deuterium/hydrogen substituted RT tags, respectively. In addition to enhancing the SERS detection of the tagged carbohydrates, the Rhodamine tagging facilitates fluorescence and mass spectrometric detection of carbohydrates. Current fluorescence sensitivity of RT-carbohydrates is ∼3 nM in concentration while the mass spectrometry (MS) sensitivity is about 1 fmol, achieved with a linear ion trap electrospray ionization (ESI)-MS instrument. Potential applications that take advantage of the high SERS, fluorescence, and MS sensitivity of this SERS tagging strategy are discussed for practical glycomic analysis where carbohydrates may be quantified with a fluorescence and SERS technique and then identified with ESI-MS techniques.

  20. The role of carbohydrates in infection strategies of enteric pathogens.

    Science.gov (United States)

    Kato, Kentaro; Ishiwa, Akiko

    2015-03-01

    Enteric pathogens cause considerable public health concerns worldwide including tropical regions. Here, we review the roles of carbohydrates in the infection strategies of various enteric pathogens including viruses, bacteria and protozoa, which infect the epithelial lining of the human and animal intestine. At host cell entry, enteric viruses, including norovirus, recognize mainly histo-blood group antigens. At the initial step of bacterial infections, carbohydrates also function as receptors for attachment. Here, we describe the function of carbohydrates in infection by Salmonella enterica and several bacterial species that produce a variety of fimbrial adhesions. During invasion by enteropathogenic protozoa, apicomplexan parasites utilize sialic acids or sulfated glycans. Carbohydrates serve as receptors for infection by these microbes; however, their usage of carbohydrates varies depending on the microbe. On the surface of the mucosal tissues of the gastrointestinal tract, various carbohydrate moieties are present and play a crucial role in infection, representing the site of infection or route of access for most microbes. During the infection and/or invasion process of the microbes, carbohydrates function as receptors for various microbes, but they can also function as a barrier to infection. One approach to develop effective prophylactic and therapeutic antimicrobial agents is to modify the drug structure. Another approach is to modify the mode of inhibition of infection depending on the individual pathogen by using and mimicking the interactions with carbohydrates. In addition, similarities in mode of infection may also be utilized. Our findings will be useful in the development of new drugs for the treatment of enteric pathogens.

  1. Root canal filling using Resilon: a review.

    LENUS (Irish Health Repository)

    Shanahan, D J

    2011-07-01

    Root canal treatment is achieved by chemo-mechanical debridement of the root canal system followed by filling. The filling material \\'entombs\\' residual bacteria and acts as a barrier which prevents the entrance of oral microorganisms and reinfection of the root canal system through microleakage. However, filling with contemporary root filling materials such as gutta-percha offers limited long-term resistance to microorganisms; as a result other materials such as Resilon have been investigated as alternatives. The aim of this review was to analyse the literature to consider whether Resilon is a suitable root canal filling material. A MEDLINE and Cochrane library search including various keyword searches identified several papers which investigated or discussed Resilon or RealSeal\\/Epiphany. Analysis of the literature demonstrated that the bulk of the literature is in vitro in nature, based largely on leakage-type studies, and demonstrates a wide variety of methodologies with conflicting findings; as a result meaningful conclusions are difficult. Within the limit of these in vitro studies Resilon appears to perform adequately in comparison to gutta-percha, however, as a result of the questionable merit of such studies, it cannot presently be considered an evidence-based alternative to the current gold standard gutta-percha. It is imperative that before Resilon is considered as a replacement material, a better understanding of the physical properties of the resin sealer and the reality of the adhesive \\'monoblock\\' are elucidated. The literature also demonstrates a paucity of quality long-term clinical outcome studies which will need to be addressed before firm conclusions can be reached.

  2. Grain dynamics in zero gravity

    Science.gov (United States)

    Werner, B. T.; Haff, P. K.

    1987-01-01

    The dynamics of granular materials has proved difficult to model, primarily because of the complications arising from inelastic losses, friction, packing, and the effect of many grains being in contact simultaneously. One interesting limit for which it was recently possible to construct a theory is that where the grain-grain interactions are dominated by binary collisions. The kinetic model of granular systems if similar to the kinetic theory of gases, except that collisional energy losses are always present in the former and must be treated explicitly. Few granular materials on Earth are describable by this limiting model, since gravity tends to collapse the grains into a high-density state where Coulombic friction effects are dominant. The planned Space Station offers an unusual opportunity to test the kinetic grain model and to explore its predictions. Without gravity, the regime of low interparticle velocities, where an elastic description of the collision is still valid, is investigated. This will allow direct interpretation by dynamical computer simulations as well as by kinetic theory.

  3. Stay-green alleles individually enhance grain yield in sorghum under drought by modifying canopy development and water uptake patterns.

    Science.gov (United States)

    Borrell, Andrew K; van Oosterom, Erik J; Mullet, John E; George-Jaeggli, Barbara; Jordan, David R; Klein, Patricia E; Hammer, Graeme L

    2014-08-01

    Stay-green is an integrated drought adaptation trait characterized by a distinct green leaf phenotype during grain filling under terminal drought. We used sorghum (Sorghum bicolor), a repository of drought adaptation mechanisms, to elucidate the physiological and genetic mechanisms underpinning stay-green. Near-isogenic sorghum lines (cv RTx7000) were characterized in a series of field and managed-environment trials (seven experiments and 14 environments) to determine the influence of four individual stay-green (Stg1-4) quantitative trait loci (QTLs) on canopy development, water use and grain yield under post-anthesis drought. The Stg QTL decreased tillering and the size of upper leaves, which reduced canopy size at anthesis. This reduction in transpirational leaf area conserved soil water before anthesis for use during grain filling. Increased water uptake during grain filling of Stg near-isogenic lines (NILs) relative to RTx7000 resulted in higher post-anthesis biomass production, grain number and yield. Importantly, there was no consistent yield penalty associated with the Stg QTL in the irrigated control. These results establish a link between the role of the Stg QTL in modifying canopy development and the subsequent impact on crop water use patterns and grain yield under terminal drought.

  4. Validation of lignocellulosic biomass carbohydrates determination via acid hydrolysis.

    Science.gov (United States)

    Zhou, Shengfei; Runge, Troy M

    2014-11-04

    This work studied the two-step acid hydrolysis for determining carbohydrates in lignocellulosic biomass. Estimation of sugar loss based on acid hydrolyzed sugar standards or analysis of sugar derivatives was investigated. Four model substrates (starch, holocellulose, filter paper and cotton) and three levels of acid/material ratios (7.8, 10.3 and 15.4, v/w) were studied to demonstrate the range of test artifacts. The method for carbohydrates estimation based on acid hydrolyzed sugar standards having the most satisfactory carbohydrate recovery and relative standard deviation. Raw material and the acid/material ratio both had significant effect on carbohydrate hydrolysis, suggesting the acid to have impacts beyond a catalyst in the hydrolysis. Following optimal procedures, we were able to reach a carbohydrate recovery of 96% with a relative standard deviation less than 3%. The carbohydrates recovery lower than 100% was likely due to the incomplete hydrolysis of substrates, which was supported by scanning electron microscope (SEM) images.

  5. Carbohydrate Mimetic Peptides for Pan Anti-Tumor Responses

    Directory of Open Access Journals (Sweden)

    Thomas eKieber-Emmons

    2014-06-01

    Full Text Available Molecular mimicry is fundamental to biology which transcends to many disciplines ranging from immune pathology to drug design. Structural characterization of molecular partners has provided insight into the origins and relative importance of complementarity in mimicry. Chemical complementarity is easy to understand; amino acid sequence similarity between peptides, for example, can lead to cross-reactivity triggering similar reactivity from their cognate receptors. However, conformational complementarity is difficult to decipher. Molecular mimicry of carbohydrates by peptides is often considered one of those. Extensive studies of innate and adaptive immune responses suggests the existence of carbohydrate mimicry, but the structural basis for this mimicry yields confounding details; peptides mimicking carbohydrates in some cases fail to exhibit both chemical and conformational mimicry. Deconvolution of these two types of complementarity in mimicry and its relationship to biological function can nevertheless lead to new therapeutics. Here, we discuss our experience in bringing a tumor-associated carbohydrate mimetic peptide to the clinic. Emphasis is placed on the rationale, the lessons learned from the methodologies to identify mimics, a perspective on the limitations of structural analysis, the biological consequences of mimicking tumor associated carbohydrate antigens and the notion of reverse engineering to develop carbohydrate mimetic peptides in vaccine design strategies to induce responses to pan-glycan antigens expressed on cancer cells.

  6. Simulation of carbohydrates, from molecular docking to dynamics in water.

    Science.gov (United States)

    Sapay, Nicolas; Nurisso, Alessandra; Imberty, Anne

    2013-01-01

    Modeling of carbohydrates is particularly challenging because of the variety of structures resulting for the high number of monosaccharides and possible linkages and also because of their intrinsic flexibility. The development of carbohydrate parameters for molecular modeling is still an active field. Nowadays, main carbohydrates force fields are GLYCAM06, CHARMM36, and GROMOS 45A4. GLYCAM06 includes the largest choice of compounds and is compatible with the AMBER force fields and associated. Furthermore, AMBER includes tools for the implementation of new parameters. When looking at protein-carbohydrate interaction, the choice of the starting structure is of importance. Such complex can be sometimes obtained from the Protein Data Bank-although the stereochemistry of sugars may require some corrections. When no experimental data is available, molecular docking simulation is generally used to the obtain protein-carbohydrate complex coordinates. As molecular docking parameters are not specifically dedicated to carbohydrates, inaccuracies should be expected, especially for the docking of polysaccharides. This issue can be addressed at least partially by combining molecular docking with molecular dynamics simulation in water.

  7. Bacterial, plant, and fungal carbohydrate structure databases: daily usage.

    Science.gov (United States)

    Toukach, Philip V; Egorova, Ksenia S

    2015-01-01

    Natural carbohydrates play important roles in living systems and therefore are used as diagnostic and therapeutic targets. The main goal of glycomics is systematization of carbohydrates and elucidation of their role in human health and disease. The amount of information on natural carbohydrates accumulates rapidly, but scientists still lack databases and computer-assisted tools needed for orientation in the glycomic information space. Therefore, freely available, regularly updated, and cross-linked databases are demanded. Bacterial Carbohydrate Structure Database (Bacterial CSDB) was developed for provision of structural, bibliographic, taxonomic, NMR spectroscopic, and other related information on bacterial and archaeal carbohydrate structures. Its main features are (1) coverage above 90%, (2) high data consistence (above 90% of error-free records), and (3) presence of manually verified bibliographic, NMR spectroscopic, and taxonomic annotations. Recently, CSDB has been expanded to cover carbohydrates of plant and fungal origin. The achievement of full coverage in the plant and fungal domains is expected in the future. CSDB is freely available on the Internet as a web service at http://csdb.glycoscience.ru. This chapter aims at showing how to use CSDB in your daily scientific practice.

  8. Carbohydrate-based nanoparticles for potential applications in medicine.

    Science.gov (United States)

    Marradi, Marco; García, Isabel; Penadés, Soledad

    2011-01-01

    Although carbohydrates are essential for life, the development of medical tools based on these important biomolecules is significantly slower than those based on proteins or nucleic acids. This chapter deals with the applications of nanoparticles decorated with carbohydrates and discusses the perspectives of their use in the field of medicine. The review is divided into two sections: diagnosis and therapy. Within these topics, the focus will be on two main types of systems: carbohydrate-coated metallic nanoparticles in which the carbohydrate ligands are "covalently" linked to a nanosized metallic cluster and polysaccharide-encapsulated metallic cores. The former glyconanoparticles (GNPs) represent a powerful chemical tool in the field of glycobiology as a specific carbohydrate can be selected to exert a concrete biological function and profile carbohydrate-based interactions. Up to now, these GNPs have been mainly used as potential anti-adhesion agents against pathogens. The opportunity of inserting multifunctionality and changing the nucleus size/material is giving birth to new targeted systems for imaging and therapy. On the other hand, nonmetallic polysaccharide-based nanoparticles have been successfully used as drug delivery carriers in addition to molecular imaging.

  9. 7 CFR 52.774 - Fill of container.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Fill of container. 52.774 Section 52.774 Agriculture... United States Standards for Grades of Canned Red Tart Pitted Cherries 1 Fill of Container § 52.774 Fill of container. (a) FDA requirements. Canned red tart pitted cherries shall meet the fill of...

  10. Deformation strain inhomogeneity in columnar grain nickel

    DEFF Research Database (Denmark)

    Wu, G.L.; Godfrey, A.; Juul Jensen, D.

    2005-01-01

    A method is presented for determination of the local deformation strain of individual grains in the bulk of a columnar grain sample. The method, based on measurement of the change in grain area of each grain, is applied to 12% cold rolled nickel. Large variations are observed in the local strain...

  11. Signal Amplification by Glyco-qPCR for Ultrasensitive Detection of Carbohydrates: Applications in Glycobiology**

    OpenAIRE

    Kwon, Seok Joon; Lee, Kyung Bok; Solakyildirim, Kemal; Masuko, Sayaka; Ly, Mellisa; Zhang, Fuming; Li, Lingyun; Dordick, Jonathan S.; Robert J Linhardt

    2012-01-01

    Tiny amounts of carbohydrates (ca. 1 zmol) can be detected quantitatively by a real-time method based on the conjugation of carbohydrates with DNA markers (see picture). The proposed method (glyco-qPCR) provides uniform, ultrasensitive detection of carbohydrates, which can be applied to glycobiology, as well as carbohydrate-based drug discovery.

  12. Carbohydrate Mimetic Peptides Augment Carbohydrate-Reactive Immune Responses in the Absence of Immune Pathology

    Energy Technology Data Exchange (ETDEWEB)

    Hennings, Leah; Artaud, Cecile; Jousheghany, Fariba; Monzavi-Karbassi, Behjatolah; Pashov, Anastas; Kieber-Emmons, Thomas, E-mail: tke@uams.edu [Winthrop P. Rockefeller Cancer Institute and Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, AR 72205 (United States)

    2011-11-11

    Among the most challenging of clinical targets for cancer immunotherapy are Tumor Associated Carbohydrate Antigens (TACAs). To augment immune responses to TACA we are developing carbohydrate mimetic peptides (CMPs) that are sufficiently potent to activate broad-spectrum anti-tumor reactivity. However, the activation of immune responses against terminal mono- and disaccharide constituents of TACA raises concerns regarding the balance between “tumor destruction” and “tissue damage”, as mono- and disaccharides are also expressed on normal tissue. To support the development of CMPs for clinical trial testing, we demonstrate in preclinical safety assessment studies in mice that vaccination with CMPs can enhance responses to TACAs without mediating tissue damage to normal cells expressing TACA. BALB/c mice were immunized with CMPs that mimic TACAs reactive with Griffonia simplicifolia lectin 1 (GS-I), and tissue reactivity of serum antibodies were compared with the tissue staining profile of GS-I. Tissues from CMP immunized mice were analyzed using hematoxylin and eosin stain, and Luxol-fast blue staining for myelination. Western blots of membranes from murine mammary 4T1 cells, syngeneic with BALB/c mice, were also compared using GS-I, immunized serum antibodies, and naive serum antibodies. CMP immunization enhanced glycan reactivities with no evidence of pathological autoimmunity in any immunized mice demonstrating that tissue damage is not an inevitable consequence of TACA reactive responses.

  13. Carbohydrate Mimetic Peptides Augment Carbohydrate-Reactive Immune Responses in the Absence of Immune Pathology

    Directory of Open Access Journals (Sweden)

    Thomas Kieber-Emmons

    2011-11-01

    Full Text Available Among the most challenging of clinical targets for cancer immunotherapy are Tumor Associated Carbohydrate Antigens (TACAs. To augment immune responses to TACA we are developing carbohydrate mimetic peptides (CMPs that are sufficiently potent to activate broad-spectrum anti-tumor reactivity. However, the activation of immune responses against terminal mono- and disaccharide constituents of TACA raises concerns regarding the balance between “tumor destruction” and “tissue damage”, as mono- and disaccharides are also expressed on normal tissue. To support the development of CMPs for clinical trial testing, we demonstrate in preclinical safety assessment studies in mice that vaccination with CMPs can enhance responses to TACAs without mediating tissue damage to normal cells expressing TACA. BALB/c mice were immunized with CMPs that mimic TACAs reactive with Griffonia simplicifolia lectin 1 (GS-I, and tissue reactivity of serum antibodies were compared with the tissue staining profile of GS-I. Tissues from CMP immunized mice were analyzed using hematoxylin and eosin stain, and Luxol-fast blue staining for myelination. Western blots of membranes from murine mammary 4T1 cells, syngeneic with BALB/c mice, were also compared using GS-I, immunized serum antibodies, and naive serum antibodies. CMP immunization enhanced glycan reactivities with no evidence of pathological autoimmunity in any immunized mice demonstrating that tissue damage is not an inevitable consequence of TACA reactive responses.

  14. Carbohydrate Mimetic Peptides Augment Carbohydrate-Reactive Immune Responses in the Absence of Immune Pathology

    Science.gov (United States)

    Hennings, Leah; Artaud, Cecile; Jousheghany, Fariba; Monzavi-Karbassi, Behjatolah; Pashov, Anastas; Kieber-Emmons, Thomas

    2011-01-01

    Among the most challenging of clinical targets for cancer immunotherapy are Tumor Associated Carbohydrate Antigens (TACAs). To augment immune responses to TACA we are developing carbohydrate mimetic peptides (CMPs) that are sufficiently potent to activate broad-spectrum anti-tumor reactivity. However, the activation of immune responses against terminal mono- and disaccharide constituents of TACA raises concerns regarding the balance between “tumor destruction” and “tissue damage”, as mono- and disaccharides are also expressed on normal tissue. To support the development of CMPs for clinical trial testing, we demonstrate in preclinical safety assessment studies in mice that vaccination with CMPs can enhance responses to TACAs without mediating tissue damage to normal cells expressing TACA. BALB/c mice were immunized with CMPs that mimic TACAs reactive with Griffonia simplicifolia lectin 1 (GS-I), and tissue reactivity of serum antibodies were compared with the tissue staining profile of GS-I. Tissues from CMP immunized mice were analyzed using hematoxylin and eosin stain, and Luxol-fast blue staining for myelination. Western blots of membranes from murine mammary 4T1 cells, syngeneic with BALB/c mice, were also compared using GS-I, immunized serum antibodies, and naive serum antibodies. CMP immunization enhanced glycan reactivities with no evidence of pathological autoimmunity in any immunized mice demonstrating that tissue damage is not an inevitable consequence of TACA reactive responses. PMID:24213131

  15. Magnesium and disturbances in carbohydrate metabolism.

    Science.gov (United States)

    Mooren, Frank C

    2015-09-01

    Magnesium is actively involved in a number of metabolic reactions as an important co-factor, with special emphasis on carbohydrate metabolism. After a brief overview of the regulation of intra- and extracellular magnesium, the present review first describes the regulatory role of magnesium in important metabolic pathways involved in energy metabolism and glycaemic control. Next the clinical significance of hypomagnesaemic conditions with regard to the management of glucose in prediabetic stages, such as insulin resistance/impaired glucose tolerance and in type 2 diabetes mellitus are characterized. Cross-sectional as well as longitudinal studies suggest that a reduced dietary magnesium intake serves as a risk factor for the incidence of both impaired glucose regulation and type 2 diabetes. Mechanisms that might be responsible for diabetes-associated hypomagnesaemia are discussed. Furthermore, the role of hypomagnesaemia in the development and progression of chronic diabetic complications are addressed. Finally, the available literature on the effects of magnesium supplementation on glycaemic control parameters during prediabetic conditions (preventive approach) as well as type 2 diabetes mellitus (therapeutic approach) are reviewed systematically. There is considerable evidence that chronic magnesium supplementation may delay the progression from impaired glucose regulation to type 2 diabetes; however, the effects of oral magnesium supplementation as an adjunct therapy for type 2 diabetes are quite heterogeneous with respect to the various measures of glycaemic control. The results of this review suggest a requirement for critical consideration of the pros and cons of magnesium replacement therapy, based on variables such as magnesium status, stage of disease and glycaemic control.

  16. STUDIES ON GENETIC PARAMETERS IN GRAIN AMARANTHUS (AMARANTHUS HYPOCHONDRIACUS L. AS INFLUENCED BY PLANT DENSITIES

    Directory of Open Access Journals (Sweden)

    Ramesh K. Selvan

    2013-04-01

    Full Text Available Selection of genotypes with adequate combination of traits with high yield at the appropriate density level increased the productivity in amaranth. The study was therefore undertaken to estimate genetic attributes of different amaranth genotypes and to identify and select genotypes with adequate trait combination for improvement in yield. In grain amaranthus (Amaranthus hypochondriacus L. ten genotypes were evaluated  for twelve characters under four plant density levels viz., very high (D1, high (D2, normal (D3 and low plant density (D4 to study the different selection parameters for grain yield and its eleven contributing morphological and quality traits. The study was conducted at College Orchard, Department of Horticulture, Pandit Jawaharlal Nehru College of Agriculture and Research Institute, TNAU, Karaikal during rabi 2007. The results revealed that the GCV was maximum in high plant density when compared to very high, normal and low plant density levels for the characters viz., fresh weight of the inflorescence, length of the rachis per inflorescence, grain yield per plant and total carbohydrates. Leaf area at 50 per cent flowering, fresh weight of the inflorescence, number of secondary branches per inflorescence and total carbohydrates are recorded high magnitude of genetic variability in combination with high heritability and genetic advance as per cent of mean in all the four plant density levels.

  17. Special interpolation to minimize grain in printer color separation

    Science.gov (United States)

    Zeng, Huanzhao

    2005-01-01

    A 3-D lookup table (LUT) approach has been developed for color separation for color inkjet printers to better control the color separation and ink limit. This method starts from the color separation for critical points, followed by 1-D interpolation to determine the color separation for grid points in critical lines, followed by 2-D interpolations to determine the color separation for grid points in critical planes, followed by 3-D interpolation to determine the color separation for the remaining grid points in the 3-D LUT. To control the spreading of K and high-density ink to some regions, such as the highlight region and the flesh tone region, we start from controlling the ink propagation in line interpolation, which is a fairly easy step, then control the ink propagation in planes, and finally control the ink propagation in 3-D interpolation. With this process, a 3-D LUT for the conversion from a printer RGB space or a virtual CMY space to an n-colorant space is built to maximize the printer gamut and to minimize grain. This paper describes the details of special interpolations to fill the grids in a 3-D color separation LUT, which includes controlling ink propagation in grids on selected lines, followed by special interpolations to minimize grain in plane-interpolation, followed by special interpolations to fill the remaining grids in the 3-D LUT.

  18. Modified carbohydrate-chitosan compounds, methods of making the same and methods of using the same

    Energy Technology Data Exchange (ETDEWEB)

    Venditti, Richard A; Pawlak, Joel J; Salam, Abdus; El-Tahlawy, Khaled Fathy

    2015-03-10

    Compositions of matter are provided that include chitosan and a modified carbohydrate. The modified carbohydrate includes a carbohydrate component and a cross linking agent. The modified carbohydrate has increased carboxyl content as compared to an unmodified counterpart carbohydrate. A carboxyl group of the modified carbohydrate is covalently bonded with an amino group of chitosan. The compositions of matter provided herein may include cross linked starch citrate-chitosan and cross linked hemicellulose citrate-chitosan, including foams thereof. These compositions yield excellent absorbency and metal chelation properties. Methods of making cross linked modified carbohydrate-chitosan compounds are also provided.

  19. Interstellar Grains: 50 Years On

    CERN Document Server

    Wickramasinghe, N Chandra

    2011-01-01

    Our understanding of the nature of interstellar grains has evolved considerably over the past half century with the present author and Fred Hoyle being intimately involved at several key stages of progress. The currently fashionable graphite-silicate-organic grain model has all its essential aspects unequivocally traceable to original peer-reviewed publications by the author and/or Fred Hoyle. The prevailing reluctance to accept these clear-cut priorities may be linked to our further work that argued for interstellar grains and organics to have a biological provenance - a position perceived as heretical. The biological model, however, continues to provide a powerful unifying hypothesis for a vast amount of otherwise disconnected and disparate astronomical data.

  20. [Apical thickness of root fillings in upper premolars. A comparison of orthograde-filled, apicoectomized and retrograde-filled teeth].

    Science.gov (United States)

    Reister, Jan Philip; Staribratova-Reister, Kamelia; Kielbassa, Andrej M

    2002-01-01

    The objective of this study was to compare the apical leakage in root canal filled, apicectomised and retrogradely filled maxillary single rooted premolars with two canals of type II configuration. For this purpose the root canals of 51 maxillary type II premolars were shaped to size ISO #55, followed by a step-back preparation to size ISO #80. Subsequently, all teeth were obturated by means of lateral condensation and randomly divided into three groups, 17 teeth each. Group I was used as a control, whereas in group II and III an apicoectomy was performed. Retrograde glass ionomer restorations (Ketac Fil) were placed additionally in group III. The specimens were exposed to methylene blue for 24 hours, then cross-sectioned, and the deepness of dye penetration was measured. Data were analyzed and tested for significant differences between the various groups (Mann-Whitney test; alpha = 0.05). The teeth in group II showed the lowest mean dye penetration. The differences were statistically significant, if compared to group I (p canals, the mean dye penetration was higher (3,557 microns +/- 1,337 microns) than in teeth without lateral canals (3,096 microns +/- 1,931 microns). The teeth in group III showed a circular dye penetration in nearly all cases. For clinical purposes, the application of retrograde glass ionomer fillings must be considered very critically.

  1. SEMICONDUCTOR TECHNOLOGY Dummy fill effect on CMP planarity

    Science.gov (United States)

    Junxiong, Zhou; Lan, Chen; Wenbiao, Ruan; Zhigang, Li; Weixiang, Shen; Tianchun, Ye

    2010-10-01

    With the use of a chemical-mechanical polishing (CMP) simulator verified by testing data from a foundry, the effect of dummy fill characteristics, such as fill size, fill density and fill shape, on CMP planarity is analyzed. The results indicate that dummy density has a significant impact on oxide erosion, and copper dishing is in proportion to dummy size. We also demonstrate that cross shape dummy fill can have the best dishing performance at the same density.

  2. Grain centre mapping - 3DXRD measurements of average grain characteristics

    DEFF Research Database (Denmark)

    Oddershede, Jette; Schmidt, Søren; Lyckegaard, Allan;

    2014-01-01

    Three-Dimensional X-ray Diraction (3DXRD) Microscopy is a generic term covering a variety of dierent techniques for characterising the mi- crostructure within the bulk of polycrystalline materials. One strategy | namely grain centre mapping | enables fast measurements of the av- erage characteris...

  3. Whole or ground millet grain provided in two supplementation frequencies for grazing beef cattle: nutritional parameters

    Directory of Open Access Journals (Sweden)

    João Marcos Beltrame Benatti

    2014-05-01

    Full Text Available The objective of this study was to evaluate the processing of millet grain provided at two supplementation frequencies for grazing beef cattle during the dry season on nutritional parameters. Five rumen-cannulated crossbred steers, with 24 to 26 months of age and average body weight of 428.6±26.06 kg, were assigned to a Latin square design (5 × 5 in a 2 × 2 + 1 factorial arrangement, as follows: two forms of millet grain (whole grains - or ground; WG - GG, two strategies of concentrate supplementation (daily distribution - 7X; or three times a week - 3X and a control treatment (mineral mixture - MM. Animals were kept in five paddocks of 0.24 ha each with Marandu grass pastures. Concentrate supplements were supplied at 2.00 and 4.66 kg/animal/day for treatments 7X and 3X, respectively. The concentrate supplementation enhanced the intake of total dry matter (DM and organic matter (OM and nutrients compared with mineral supplementation. No differences were found between dry matter intake and forage organic matter intake. The values of digestibility coefficients of DM, OM, ether extract, total carbohydrates and non-fiber carbohydrates were increased by concentrate supplementation. Regarding concentrations of rumen ammonia nitrogen, the interactions among time × treatment × day and day × treatment had effects on the measurements of ruminal pH. Plasma urea nitrogen, urinary urea excretion and urinary urea N excretion differed only between MM treatment and the others, with no interference of grain physical form and supplementation frequency on those variables. The millet grain processing does not alter forage intake, but improves digestibility. Daily supplementation increases digestibility of dry matter and neutral detergent fiber.

  4. Ultrafast carriers dynamics in filled-skutterudites

    Energy Technology Data Exchange (ETDEWEB)

    Guo, Liang; Xu, Xianfan, E-mail: xxu@purdue.edu [School of Mechanical Engineering and Birck Nanotechnology Center, Purdue University, West Lafayette, Indiana 47907 (United States); Salvador, James R. [Chemical and Materials Systems Laboratory, GM Global R and D, Warren, Michigan 48090 (United States)

    2015-06-08

    Carrier dynamics of filled-skutterudites, an important class of thermoelectric materials, is investigated using ultrafast optical spectroscopy. By tuning the wavelength of the probe laser, charge transfers at different electronic energy levels are interrogated. Analysis based on the Kramers-Kronig relation explains the complex spectroscopy data, which is mainly due to band filling caused by photo-excited carriers and free carrier absorption. The relaxation time of hot carriers is found to be about 0.4–0.6 ps, depending on the electronic energy level, and the characteristic time for carrier-phonon equilibrium is about 0.95 ps. These studies of carrier dynamics, which fundamentally determines the transport properties of thermoelectric material, can provide guidance for the design of materials.

  5. The Berkeley gas-filled separator

    CERN Document Server

    Ninov, V; McGrath, C A

    1998-01-01

    The BGS is being constructed at the 88-Inch Cyclotron at LBNL in Berkeley. The magnetic configuration of the BGS will allow a large angular acceptance and good suppression of primary beam particles. BGS operates as a mass spectrometer with a A/ Delta A approximately =200 and as a gas filled separator at pressures between 0.1-50 hPa. The reaction products recoiling off a thin target will be collected with efficiencies from 10-80at the focal plane. A Monte Carlo simulation program of the ion transport through the gas-filled magnets in combination of 3-dimensional TOSCA field maps has been developed and reproduces closely the experimental behavior of BGS. (9 refs).

  6. Text-Filled Stacked Area Graphs

    DEFF Research Database (Denmark)

    Kraus, Martin

    2011-01-01

    Text can add a significant amount of detail and value to an information visualization. In particular, it can integrate more of the data that a visualization is based on, and it can also integrate information that is personally relevant to readers of a visualization. This may influence readers...... to consider a visualization a detailed enrichment of their personal experience instead of an abstract representation of anonymous numbers. However, the integration of textual detail into a visualization is often very challenging. This work discusses one particular approach to this problem, namely text......-filled stacked area graphs; i.e., graphs that feature stacked areas that are filled with small-typed text. Since these graphs allow for computing the text layout automatically, it is possible to include large amounts of textual detail with very little effort. We discuss the most important challenges and some...

  7. Underground void filling by cemented mill tailings

    Institute of Scientific and Technical Information of China (English)

    Choudhary Bhanwar Singh; Kumar Santosh

    2013-01-01

    Underground mining always create voids. These voids can cause subsidence of surface. So it is always a demand to fill the void in such a manner that the effect of underground mining can be minimized. Void filling using mill tailings especially in metal mining is one of the best techniques. The tailings produced in milling process have traditionally been disposed in tailing ponds creating a waste disposal and environ-mental problems in terms of land degradation, air and water pollution, etc. This disposal practice is more acute in the metal milling industry where the fine grinding, required for value liberation, results in the production of very fine tailings in large percentage. This paper includes discussions on the effectiveness of different paste mixes with varying cement contents in paste backfilling operations. The results revealed that material composition and use of super plasticizer strongly influenced the strength of cemented backfill.

  8. Ultrafast carriers dynamics in filled-skutterudites

    Science.gov (United States)

    Guo, Liang; Xu, Xianfan; Salvador, James R.

    2015-06-01

    Carrier dynamics of filled-skutterudites, an important class of thermoelectric materials, is investigated using ultrafast optical spectroscopy. By tuning the wavelength of the probe laser, charge transfers at different electronic energy levels are interrogated. Analysis based on the Kramers-Kronig relation explains the complex spectroscopy data, which is mainly due to band filling caused by photo-excited carriers and free carrier absorption. The relaxation time of hot carriers is found to be about 0.4-0.6 ps, depending on the electronic energy level, and the characteristic time for carrier-phonon equilibrium is about 0.95 ps. These studies of carrier dynamics, which fundamentally determines the transport properties of thermoelectric material, can provide guidance for the design of materials.

  9. Conjectural Equilibrium in Water-filling Games

    CERN Document Server

    Su, Yi

    2008-01-01

    This paper considers a non-cooperative game in which competing users sharing a frequency-selective interference channel selfishly optimize their power allocation in order to improve their achievable rates. Previously, it was shown that a user having the knowledge of its opponents' channel state information can make foresighted decisions and substantially improve its performance compared with the case in which it deploys the conventional iterative water-filling algorithm, which does not exploit such knowledge. This paper discusses how a foresighted user can acquire this knowledge by modeling its experienced interference as a function of its own power allocation. To characterize the outcome of the multi-user interaction, the conjectural equilibrium is introduced, and the existence of this equilibrium for the investigated water-filling game is proved. Interestingly, both the Nash equilibrium and the Stackelberg equilibrium are shown to be special cases of the generalization of conjectural equilibrium. We develop...

  10. Impacts of nighttime post-anthesis warming on rice productivity and grain quality in East China

    Directory of Open Access Journals (Sweden)

    Wenjun Dong

    2014-02-01

    Full Text Available The impacts of nighttime post-anthesis warming on rice productivity and grain quality in East China were evaluated for two cultivars, II You 128, an indica rice, and Wuyunjing 7, a japonica rice. Warming by 3.0 °C stimulated the nighttime respiration rate and decreased the photosynthesis rate, resulting in significant decreases of 21.2% and 24.9% in aboveground biomass accumulation for II You 128 and Wuyunjing 7, respectively. Warming significantly reduced the rates of seed setting and grain filling, especially of inferior kernels (those lower in panicles, while the filling rate of superior kernels remained almost unchanged. As a result, 1000-grain weight and grain yield were respectively 3.7% and 30.0% lower for II You 128 and 12.8% and 34.3% for Wuyunjing 7 in warmed plots than in the unwarmed control. Nighttime warming also significantly reduced the grain milling and appearance quality of both varieties. More negative effects of warming on inferior than on superior kernels were found. The above results have important implications for rice variety cultivation in East China.

  11. Impacts of nighttime post-anthesis warming on rice productivity and grain quality in East China

    Institute of Scientific and Technical Information of China (English)

    Wenjun; Dong; Jin; Chen; Lili; Wang; Yunlu; Tian; Bin; Zhang; Yongcai; Lai; Ying; Meng; Chunrong; Qian; Jia; Guo

    2014-01-01

    The impacts of nighttime post-anthesis warming on rice productivity and grain quality in East China were evaluated for two cultivars, II You 128, an indica rice, and Wuyunjing 7, a japonica rice. Warming by 3.0 °C stimulated the nighttime respiration rate and decreased the photosynthesis rate, resulting in significant decreases of 21.2% and 24.9% in aboveground biomass accumulation for II You 128 and Wuyunjing 7, respectively. Warming significantly reduced the rates of seed setting and grain filling, especially of inferior kernels(those lower in panicles), while the filling rate of superior kernels remained almost unchanged. As a result, 1000-grain weight and grain yield were respectively 3.7% and 30.0% lower for II You 128 and 12.8% and 34.3% for Wuyunjing 7 in warmed plots than in the unwarmed control. Nighttime warming also significantly reduced the grain milling and appearance quality of both varieties. More negative effects of warming on inferior than on superior kernels were found. The above results have important implications for rice variety cultivation in East China.

  12. Carbohydrate Supplementation Influences Serum Cytokines after Exercise under Hypoxic Conditions

    Directory of Open Access Journals (Sweden)

    Aline Venticinque Caris

    2016-11-01

    Full Text Available Introduction: Exercise performed at the hypoxia equivalent of an altitude of 4200 m is associated with elevated inflammatory mediators and changes in the Th1/Th2 response. By contrast, supplementation with carbohydrates has an anti-inflammatory effect when exercise is performed under normoxic conditions. The objective of this study was to evaluate the effect of carbohydrate supplementation on cytokines and cellular damage markers after exercise under hypoxic conditions at a simulated altitude of 4200 m. Methods: Seven adult male volunteers who exercised for 60 min at an intensity of 50% VO2Peak were randomly evaluated under three distinct conditions; normoxia, hypoxia and hypoxia + carbohydrate supplementation. Blood samples were collected at rest, at the end of exercise and after 60 min of recovery. To evaluate hypoxia + carbohydrate supplementation, volunteers received a solution of 6% carbohydrate (maltodextrin or a placebo (strawberry-flavored Crystal Light®; Kraft Foods, Northfield, IL, USA every 20 min during exercise and recovery. Statistical analyses comprised analysis of variance, with a one-way ANOVA followed by the Tukey post hoc test with a significance level of p < 0.05. Results: Under normoxic and hypoxic conditions, there was a significant increase in the concentration of IL-6 after exercise and after recovery compared to at rest (p < 0.05, while in the hypoxia + carbohydrate group, there was a significant increase in the concentration of IL-6 and TNF-α after exercise compared to at rest (p < 0.05. Furthermore, under this condition, TNF-α, IL-2 and the balance of IL-2/IL-4 were increased after recovery compared to at rest (p < 0.05. Conclusion: We conclude that carbohydrate supplementation modified the IL-6 and TNF-α serum concentrations and shifted the IL-2/IL-4 balance towards Th1 in response without glycemic, glutaminemia and cell damage effects.

  13. Moon - 'Ghost' craters formed during Mare filling.

    Science.gov (United States)

    Cruikshank, D. P.; Hartmann, W. K.; Wood, C. A.

    1973-01-01

    This paper discusses formation of 'pathological' cases of crater morphology due to interaction of craters with molten lavas. Terrestrial observations of such a process are discussed. In lunar maria, a number of small impact craters (D less than 10 km) may have been covered by thin layers of fluid lavas, or formed in molten lava. Some specific lunar examples are discussed, including unusual shallow rings resembling experimental craters deformed by isostatic filling.

  14. Full-waveform inversion: Filling the gaps

    KAUST Repository

    Beydoun, Wafik B.

    2015-09-01

    After receiving an outstanding response to its inaugural workshop in 2013, SEG once again achieved great success with its 2015 SEG Middle East Workshop, “Full-waveform inversion: Filling the gaps,” which took place 30 March–1 April 2015 in Abu Dhabi, UAE. The workshop was organized by SEG, and its partner sponsors were Saudi Aramco (gold sponsor), ExxonMobil, and CGG. Read More: http://library.seg.org/doi/10.1190/tle34091106.1

  15. Modelling of dosator filling and discharge

    OpenAIRE

    Angulo Pinzon, Oscar Andres

    2012-01-01

    Dosators (and other machines operating on generally similar principles) are widely used in the pharmaceutical industry for dosing products that are delivered to the customer in powder form (i.e. capsules and dry inhaled powder applications). However a significant problem for this technology is the ability to predict how accurately and reliably, new formulations can be dosed from these machines prior to scale-up for manufacture. Dosator filling machines have been on the market for over for...

  16. Air filled porosity in composting processes

    Energy Technology Data Exchange (ETDEWEB)

    Ruggieri, L.; Gea, T.; Artola, A.; Sanchez, A.

    2009-07-01

    As it is widely known, the composting process consists in the aerobic decomposition of the biodegradable organic matter present in different types of solid wastes. Water and oxygen are necessary for the biological activity of microorganisms involved in the composting process and their availability is directly related to the total and the air filled porosity (AFP). Maintaining adequate AFP level satisfies the oxygen content requirement to achieve the desired composting conditions and thus, tho enhance biological activity. (Author)

  17. Spin and charge necklaces at commensurate filling

    Energy Technology Data Exchange (ETDEWEB)

    Kikoin, K [School of Physics and Astronomy, Tel-Aviv University, Tel-Aviv 69978 (Israel); Kiselev, M N, E-mail: konstk@post.tau.ac.i [The Abdus Salam International Center for Theoretical Physics, Strada Costiera 11, 34014 Trieste (Italy)

    2009-03-01

    The charge and spin properties of spin chains decorated with dimers and closed trimers (equilateral triangles) with commensurate partial filling (1/4 and 1/3, respectively) are considered. It is shown that due to the charge separation both systems prefer the ground state with even occupation per elementary cell, where the spin spectrum possesses the Haldane gap for negative spin exchange and magnon-like for positive coupling. The charge spectrum is always gapped.

  18. Form Filling with Self-Compacting Concrete

    DEFF Research Database (Denmark)

    Thrane, Lars Nyholm

    2002-01-01

    This paper describes a newly started Ph.D. project with the aim of simulating the form filling ability of Self-Compacting Concrete (SCC) taking into account the form geometry, reinforcement configuration, casting technique, and the rheological properties of the concrete. Comparative studies...... to be taken into account. Furthermore investigations will be carried out to verify and further develop models based on the composite theory describing the effect of mixture composition on the rheological properties and stability of fresh SCC....

  19. Statistical evaluation of metal fill widths for emulated metal fill in parasitic extraction methodology

    Science.gov (United States)

    J-Me, Teh; Noh, Norlaili Mohd.; Aziz, Zalina Abdul

    2015-05-01

    In the chip industry today, the key goal of a chip development organization is to develop and market chips within a short time frame to gain foothold on market share. This paper proposes a design flow around the area of parasitic extraction to improve the design cycle time. The proposed design flow utilizes the usage of metal fill emulation as opposed to the current flow which performs metal fill insertion directly. By replacing metal fill structures with an emulation methodology in earlier iterations of the design flow, this is targeted to help reduce runtime in fill insertion stage. Statistical design of experiments methodology utilizing the randomized complete block design was used to select an appropriate emulated metal fill width to improve emulation accuracy. The experiment was conducted on test cases of different sizes, ranging from 1000 gates to 21000 gates. The metal width was varied from 1 x minimum metal width to 6 x minimum metal width. Two-way analysis of variance and Fisher's least significant difference test were used to analyze the interconnect net capacitance values of the different test cases. This paper presents the results of the statistical analysis for the 45 nm process technology. The recommended emulated metal fill width was found to be 4 x the minimum metal width.

  20. Effects of Uniconazole on Nitrogen Metabolism and Grain Protein Content of Rice

    Institute of Scientific and Technical Information of China (English)

    XIANG Zu-fen; YANG Wen-yu; REN Wan-jun; WANG Xiao-chun

    2005-01-01

    The effects of uniconazole by soaking seeds and spraying leaves at booting stage with different concentrations (0, 20 and40 mg/kg) on the nitrogen metabolism of flag leaf and grains after flowering, and rice grain protein content and yield were studied withhybrid rice combination Shanyou 63. Under uniconazole treatment, the soluble protein content in flag leaf was increased in early andmiddle period of grain filling, but this content was nearly the same as or even lower than that of control at maturity; Glutaminesynthetase activity in superior and inferior grains and non-protein nitrogen content in superior grains at early stage of graindevelopment were promoted, and moreover, the transforming speed from non-protein nitrogen to protein nitrogen was accelerated;Non-protein nitrogen content was lower than that of control at maturity, but protein nitrogen content at each stage was higher thanthose of control; Protein nitrogen content in superior and inferior grains and protein nitrogen absolutely accumulative content in agrain both were enhanced and protein content and yield in rice grain were raised. The application of uniconazole by soaking seedsand spraying leaves raised crude protein content by an average of 7.2% and 8.3%, and protein yield by an average of 13.1% and13.4%, respectively.

  1. Cavity filling water control below aerator devices

    Institute of Scientific and Technical Information of China (English)

    钱尚拓; 吴建华; 马飞; 徐建荣; 彭育; 汪振

    2014-01-01

    With the rapid development of high dam projects within China, the dragon-drop-tail spillway tunnel is introduced and widely used. In view of the high water head and the large flow velocity on the dragon-drop-tail section, aerator devices are usually placed for the cavitation damage control. For the device placed in its initial position, it is a serious concern to design a suitable flow regime of the cavity and to control the cavity filling water due to the large flow depth and the low Froude number through this aera-tor. In this study, the relationships between the geometries of the aerator device and the jet impact angle of the lower trajectory of the flow are theoretically analyzed with/without a local slope. Nine test cases with different geometries are designed, the effectiveness of the filling water control is experimentally investigated under different operation conditions, and two criteria of the local slope design are proposed. It is concluded that the cavity flow regime and the filling water can be improved if a small impact angle and some sui-table geometries of the local slope are designed.

  2. Establishment of growth medium and quantification of pollen grains of olive cultivars in Brazil's subtropical areas

    Directory of Open Access Journals (Sweden)

    Luiz Fernando de Oliveira da Silva

    2015-01-01

    Full Text Available ABSTRACT Pollen grain germination in vitro indicates viability and consequently provides information related to fruit set. It also assists in the development of hybrids. Along with a suitable species, a standard culture medium is essential for evaluating pollen viability. It should contain a gelling agent consisting of carbohydrates and enhancer elements as well as have the correct pH, temperature, and incubation time. The objective of this study was to optimise the culture medium, determine the pollen germination capacity, and quantify the number of pollen grains per flower of certain olive tree cultivars. A basic sequential culture medium for pollen grain germination was determined, always utilizing the best result from the previous experiment to continue the sequence.The factorial treatment arrangement was: 1 agar versus boric acid; 2 pH versus sucrose; 3 calcium nitrate versus magnesium sulfate. After determining the culture medium components, two experiments were conducted evaluating temperature and incubation time. Another experiment evaluated both the germination percentage and the number of flower pollen grains of 28 cultivars. The culture medium should be composed of 4 g∙L-1 of agar, 90 g∙L-1 of sucrose, and 400 mg∙L-1 of boric acid with a pH adjusted to 5.79 and an incubation time of 60 h at 28 °C. The Manzanilla 215 cultivar had the highest germination rate while Ascolano 315 presented the highest number of pollen grains per flower.

  3. AGB stars and presolar grains

    CERN Document Server

    Busso, M; Maiorca, E; Palmerini, S

    2013-01-01

    Among presolar materials recovered in meteorites, abundant SiC and Al$_{2}$O$_{3}$ grains of AGB origins were found. They showed records of C, N, O, $^{26}$Al and s-element isotopic ratios that proved invaluable in constraining the nucleosynthesis models for AGB stars \\cite{zin,gal}. In particular, when these ratios are measured in SiC grains, they clearly reveal their prevalent origin in cool AGB circumstellar envelopes and provide information on both the local physics and the conditions at the nucleosynthesis site (the H- and He-burning layers deep inside the structure). Among the properties ascertained for the main part of the SiC data (the so-called {\\it mainstream} ones), we mention a large range of $^{14}$N/$^{15}$N ratios, extending below the solar value \\cite{mar}, and $^{12}$C/$^{13}$C ratios $\\gtrsim$ 30. Other classes of grains, instead, display low carbon isotopic ratios ($\\gtrsim 10$) and a huge dispersion for N isotopes, with cases of large $^{15}$N excess. In the same grains, isotopes currently...

  4. Distal delta-plain successions : architecture and lithofacies of organics and lake fills in the Holocene Rhine-Meuse delta, The Netherlands

    NARCIS (Netherlands)

    Bos, I.J.

    2010-01-01

    The objective of this thesis is to analyze and explain the architecture, facies distribution, age and origin of coarse-grained overbank deposits, with special attention for organic-clastic lake fills, and organics in the distal Holocene Rhine-Meuse delta plain. In order to depict the influence of la

  5. Supercurrent through cuprate grain boundaries in the presence of strong correlations

    Energy Technology Data Exchange (ETDEWEB)

    Wolf, Fabian Alexander; Graser, Siegfried; Loder, Florian; Kopp, Thilo [Center for Electronic Correlations and Magnetism, Institute of Physics, University of Augsburg, 86135 Augsburg (Germany)

    2012-07-01

    Strong correlations are known to severely reduce the mobility of charge carriers near half-filling and thus have an important influence on the current carrying properties of grain boundaries in high-T{sub c} cuprates. We apply a Gutzwiller method to investigate the critical current through microscopically reconstructed grain boundaries for a wide range of misalignment angles. In good agreement with experimental data, we find a reduction of the current by one order of magnitude as compared to an analogous weak coupling evaluation. This reduction emerges from the interplay of charge fluctuations and strong correlations.

  6. Predicting water-soluble carbohydrates and ethanol-soluble carbohydrates in cool-season grasses with near-infrared reflectance spectroscopy

    Science.gov (United States)

    Grazing animals may require a high or low total nonstructural carbohydrate diet for optimal health and production. Understanding how nonstructural carbohydrates fluctuate in Kentucky pastures and being able to quantify and monitor nonstructural carbohydrates in a timely manner will greatly aid in m...

  7. Selectivity switch in the catalytic functionalization of nonprotected carbohydrates: selective synthesis in the presence of anomeric and structurally similar carbohydrates under mild conditions.

    Science.gov (United States)

    Muramatsu, Wataru; Takemoto, Yuki

    2013-03-15

    A catalytic process for the chemo- and regioselective functionalization of nonprotected carbohydrates has been developed. This novel process allows selective thiocarbonylation, acylation, and sulfonylation of a particular hydroxy group in a particular carbohydrate in the simultaneous presence of structurally similar carbohydrates such as anomers. In addition, the chemoselectivity can be switched by regulating only the length of the alkyl chain in the organotin catalyst.

  8. Concepts on Low Temperature Mechanical Grain Growth

    Energy Technology Data Exchange (ETDEWEB)

    Sharon, John Anthony [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Metallurgy and Materials Joining Dept.; Boyce, Brad Lee [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Metallurgy and Materials Joining Dept.

    2013-11-01

    In metals, as grain size is reduced below 100nm, conventional dislocation plasticity is suppressed resulting in improvements in strength, hardness, and wears resistance. Existing and emerging components use fine grained metals for these beneficial attributes. However, these benefits can be lost in service if the grains undergo growth during the component’s lifespan. While grain growth is traditionally viewed as a purely thermal process that requires elevated temperature exposure, recent evidence shows that some metals, especially those with nanocrystalline grain structure, can undergo grain growth even at room temperature or below due to mechanical loading. This report has been assembled to survey the key concepts regarding how mechanical loads can drive grain coarsening at room temperature and below. Topics outlined include the atomic level mechanisms that facilitate grain growth, grain boundary mobility, and the impact of boundary structure, loading scheme, and temperature.

  9. AGB stars and presolar grains

    Energy Technology Data Exchange (ETDEWEB)

    Busso, M.; Trippella, O. [INFN and University of Perugia, Perugia (Italy); Maiorca, E. [INAF - Arcetri Astrophysical Observatory, Firenze, Italy and INFN - Section of Perugia, Perugia (Italy); Palmerini, S. [Departamento de Fìsica Teòrica y del Cosmsos, Universidad de Granada, Granada (Spain)

    2014-05-09

    Among presolar materials recovered in meteorites, abundant SiC and Al{sub 2}O{sub 3} grains of AGB origins were found. They showed records of C, N, O, {sup 26}Al and s-element isotopic ratios that proved invaluable in constraining the nucleosynthesis models for AGB stars [1, 2]. In particular, when these ratios are measured in SiC grains, they clearly reveal their prevalent origin in cool AGB circumstellar envelopes and provide information on both the local physics and the conditions at the nucleosynthesis site (the H- and He-burning layers deep inside the structure). Among the properties ascertained for the main part of the SiC data (the so-called mainstream ones), we mention a large range of {sup 14}N/{sup 15}N ratios, extending below the solar value [3], and {sup 12}C/{sup 13}C ratios ≳ 30. Other classes of grains, instead, display low carbon isotopic ratios (≳ 10) and a huge dispersion for N isotopes, with cases of large {sup 15}N excess. In the same grains, isotopes currently feeded by slow neutron captures reveal the characteristic pattern expected from this process at an efficiency slightly lower than necessary to explain the solar main s-process component. Complementary constraints can be found in oxide grains, especially Al{sub 2}O{sub 3} crystals. Here, the oxygen isotopes and the content in {sup 26}Al are of a special importance for clarifying the partial mixing processes that are known to affect evolved low-mass stars. Successes in modeling the data, as well as problems in explaining some of the mentioned isotopic ratios through current nucleosynthesis models are briefly outlined.

  10. Grain Refinement of Deoxidized Copper

    Science.gov (United States)

    Balart, María José; Patel, Jayesh B.; Gao, Feng; Fan, Zhongyun

    2016-10-01

    This study reports the current status of grain refinement of copper accompanied in particular by a critical appraisal of grain refinement of phosphorus-deoxidized, high residual P (DHP) copper microalloyed with 150 ppm Ag. Some deviations exist in terms of the growth restriction factor ( Q) framework, on the basis of empirical evidence reported in the literature for grain size measurements of copper with individual additions of 0.05, 0.1, and 0.5 wt pct of Mo, In, Sn, Bi, Sb, Pb, and Se, cast under a protective atmosphere of pure Ar and water quenching. The columnar-to-equiaxed transition (CET) has been observed in copper, with an individual addition of 0.4B and with combined additions of 0.4Zr-0.04P and 0.4Zr-0.04P-0.015Ag and, in a previous study, with combined additions of 0.1Ag-0.069P (in wt pct). CETs in these B- and Zr-treated casts have been ascribed to changes in the morphology and chemistry of particles, concurrently in association with free solute type and availability. No further grain-refining action was observed due to microalloying additions of B, Mg, Ca, Zr, Ti, Mn, In, Fe, and Zn (~0.1 wt pct) with respect to DHP-Cu microalloyed with Ag, and therefore are no longer relevant for the casting conditions studied. The critical microalloying element for grain size control in deoxidized copper and in particular DHP-Cu is Ag.

  11. Cool Down!——The State Administration of Grain Lower Expectation of Grain Price

    Institute of Scientific and Technical Information of China (English)

    He Jun

    2010-01-01

    @@ The rising grain price in the first half of the year,coupled with the news that China's summer grain output fell for the first time in seven years,has enhanced market expectation for the grain price to rise.

  12. Pharmaceutical, cosmeceutical, and traditional applications of marine carbohydrates.

    Science.gov (United States)

    Ahmed, Abdul Bakrudeen Ali; Adel, Mohaddeseh; Karimi, Pegah; Peidayesh, Mahvash

    2014-01-01

    Marine carbohydrates are most important organic molecules made by photosynthetic organisms. It is very essential for humankind: the role in being an energy source for the organism and they are considered as an important dissolve organic compound (DOC) in marine environment's sediments. Carbohydrates found in different marine environments in different concentrations. Polysaccharides of carbohydrates play an important role in various fields such as pharmaceutical, food production, cosmeceutical, and so on. Marine organisms are good resources of nutrients, and they are rich carbohydrate in sulfated polysaccharide. Seaweeds (marine microalgae) are used in different pharmaceutical industries, especially in pharmaceutical compound production. Seaweeds have a significant amount of sulfated polysaccharides, which are used in cosmeceutical industry, besides based on the biological applications. Since then, traditional people, cosmetics products, and pharmaceutical applications consider many types of seaweed as an important organism used in food process. Sulfated polysaccharides containing seaweed have potential uses in the blood coagulation system, antiviral activity, antioxidant activity, anticancer activity, immunomodulating activity, antilipidepic activity, etc. Some species of marine organisms are rich in polysaccharides such as sulfated galactans. Various polysaccharides such as agar and alginates, which are extracted from marine organisms, have several applications in food production and cosmeceutical industries. Due to their high health benefits, compound-derived extracts of marine polysaccharides have various applications and traditional people were using them since long time ago. In the future, much attention is supposed to be paid to unraveling the structural, compositional, and sequential properties of marine carbohydrate as well.

  13. Carbohydrate-Mimetic Peptides for Pan Anti-Tumor Responses

    Science.gov (United States)

    Kieber-Emmons, Thomas; Saha, Somdutta; Pashov, Anastas; Monzavi-Karbassi, Behjatolah; Murali, Ramachandran

    2014-01-01

    Molecular mimicry is fundamental to biology and transcends to many disciplines ranging from immune pathology to drug design. Structural characterization of molecular partners has provided insight into the origins and relative importance of complementarity in mimicry. Chemical complementarity is easy to understand; amino acid sequence similarity between peptides, for example, can lead to cross-reactivity triggering similar reactivity from their cognate receptors. However, conformational complementarity is difficult to decipher. Molecular mimicry of carbohydrates by peptides is often considered one of those. Extensive studies of innate and adaptive immune responses suggests the existence of carbohydrate mimicry, but the structural basis for this mimicry yields confounding details; peptides mimicking carbohydrates in some cases fail to exhibit both chemical and conformational mimicry. Deconvolution of these two types of complementarity in mimicry and its relationship to biological function can nevertheless lead to new therapeutics. Here, we discuss our experience examining the immunological aspects and implications of carbohydrate–peptide mimicry. Emphasis is placed on the rationale, the lessons learned from the methodologies to identify mimics, a perspective on the limitations of structural analysis, the biological consequences of mimicking tumor-associated carbohydrate antigens, and the notion of reverse engineering to develop carbohydrate-mimetic peptides in vaccine design strategies to induce responses to glycan antigens expressed on cancer cells. PMID:25071769

  14. GlycoCT-a unifying sequence format for carbohydrates.

    Science.gov (United States)

    Herget, S; Ranzinger, R; Maass, K; Lieth, C-W V D

    2008-08-11

    As part of the EUROCarbDB project (www.eurocarbdb.org) we have carefully analyzed the encoding capabilities of all existing carbohydrate sequence formats and the content of publically available structure databases. We have found that none of the existing structural encoding schemata are capable of coping with the full complexity to be expected for experimentally derived structural carbohydrate sequence data across all taxonomic sources. This gap motivated us to define an encoding scheme for complex carbohydrates, named GlycoCT, to overcome the current limitations. This new format is based on a connection table approach, instead of a linear encoding scheme, to describe the carbohydrate sequences, with a controlled vocabulary to name monosaccharides, adopting IUPAC rules to generate a consistent, machine-readable nomenclature. The format uses a block concept to describe frequently occurring special features of carbohydrate sequences like repeating units. It exists in two variants, a condensed form and a more verbose XML syntax. Sorting rules assure the uniqueness of the condensed form, thus making it suitable as a direct primary key for database applications, which rely on unique identifiers. GlycoCT encompasses the capabilities of the heterogeneous landscape of digital encoding schemata in glycomics and is thus a step forward on the way to a unified and broadly accepted sequence format in glycobioinformatics.

  15. Phosphorous Nutritional Level, Carbohydrate Reserves and Flower Quality in Olives

    Science.gov (United States)

    Erel, Ran; Yermiyahu, Uri; Yasuor, Hagai; Cohen Chamus, Dan; Schwartz, Amnon; Ben-Gal, Alon; Dag, Arnon

    2016-01-01

    The olive tree is generally characterized by relatively low final fruit set consequential to a significant rate of undeveloped pistils, pistil abortion, and flower and fruitlet abscission. These processes are acknowledged to be governed by competition for resources between the developing vegetative and reproductive organs. To study the role of phosphorus (P) nutritional level on reproductive development, trees were grown under four levels of P for three years in large containers. Phosphorus nutritional level was positively related to rate of reproductive bud break, inflorescence weight, rate of hermaphrodite flowers, pistil weight, fruitlet persistence, fruit set and the consequential total number of fruits. The positive impact of P nutrition on the productivity parameters was not related to carbohydrate reserves or to carbohydrate transport to the developing inflorescence. Phosphorous deficient trees showed significant impairment of assimilation rate, and yet, carbohydrates were accumulated in inflorescences at levels comparable to or higher than trees receiving high P. In contrast to female reproductive organs, pollen viability was consistently higher in P deficient trees, possibly due to the enhanced carbohydrate availability. Overall, the positive effect of P on female reproductive development was found to be independent of the total carbohydrate availability. Hence, P is speculated to have a direct influence on reproductive processes. PMID:27907133

  16. Interaction of carbohydrates with alcohol dehydrogenase: Effect on enzyme activity.

    Science.gov (United States)

    Jadhav, Swati B; Bankar, Sandip B; Granström, Tom; Ojamo, Heikki; Singhal, Rekha S; Survase, Shrikant A

    2015-09-01

    Alcohol dehydrogenase was covalently conjugated with three different oxidized carbohydrates i.e., glucose, starch and pectin. All the carbohydrates inhibited the enzyme. The inhibition was studied with respect to the inhibition rate constant, involvement of thiol groups in the binding, and structural changes in the enzyme. The enzyme activity decreased to half of its original activity at the concentration of 2 mg/mL of pectin, 4 mg/mL of glucose and 10 mg/mL of starch within 10 min at pH 7. This study showed oxidized pectin to be a potent inhibitor of alcohol dehydrogenase followed by glucose and starch. Along with the aldehyde-amino group interaction, thiol groups were also involved in the binding between alcohol dehydrogenase and carbohydrates. The structural changes occurring on binding of alcohol dehydrogenase with oxidized carbohydrates was also confirmed by fluorescence spectrophotometry. Oxidized carbohydrates could thus be used as potential inhibitors of alcohol dehydrogenase.

  17. Differential appearance of isoforms and cultivar variation in protein temporal profiles revealed in the maturing barley grain proteome

    DEFF Research Database (Denmark)

    Finnie, Christine; Bak-Jensen, K.S.; Laugesen, Sabrina

    2006-01-01

    -peroxiredoxin isoform was identified in three spots, one present throughout grain filling, one appearing during desiccation and one observed only in mature seeds. This suggested post-translational modification of the protein to different degrees during seed maturation. Distinct isoforms of several proteins were...

  18. Is the Proportion of Carbohydrate Intake Associated with the Incidence of Diabetes Complications?—An Analysis of the Japan Diabetes Complications Study

    Directory of Open Access Journals (Sweden)

    Chika Horikawa

    2017-02-01

    Full Text Available The appropriate proportions of macronutritional intake have been controversial in medical nutritional therapy for diabetes, and evidence of the effects of carbohydrate consumption on diabetes complications in prospective settings is sparse. We investigated the relationships between proportions of carbohydrate intake as the % of total energy and diabetes complications in a nationwide cohort of Japanese patients with type 2 diabetes aged 40–70 years with hemoglobin A1c ≥6.5%. The analysis was of 1516 responders to a baseline dietary survey assessed by the Food Frequency Questionnaire based on food groups. Primary outcomes were times to overt nephropathy, diabetic retinopathy, and cardiovascular disease (CVD after 8 years. Hazard ratios (HRs for proportions of carbohydrate intake were estimated by Cox regression adjusted for confounders. High carbohydrate intake was significantly related to higher intakes of grain, fruits, and sweets/snacks and lower intakes of soybean and soy products, vegetables, seaweed, meat and processed meat, fish and processed fish, eggs, milk and dairy products, oil, and alcoholic beverages. During the eight-year follow-up, there were 81, 275, and 129 events of overt nephropathy, diabetic retinopathy, and CVD, respectively. After adjustment for confounders, HRs for complications in patients with carbohydrate intake in the second or third tertiles (51.0%–56.4% and ≥56.5%, respectively compared with carbohydrate intake in the first tertile (<50.9%, referent were analyzed. No significant associations were shown in the second and third tertiles relative to first tertile (overt nephropathy: 1.05 (95% Confidence Interval, 0.54–2.06 and 0.98 (0.40–2.44; diabetic retinopathy: 1.30 (0.90–1.88 and 1.30 (0.78–2.15; and CVD: 0.95 (0.55–1.63 and 1.37 (0.69–2.72. By exploring potentially nonlinear relationships, trends for the incidence of diabetes complications according to proportions of carbohydrate intake were not

  19. On-capillary sample cleanup method for the electrophoretic determination of carbohydrates in juice samples.

    Science.gov (United States)

    Morales-Cid, Gabriel; Simonet, Bartolomé M; Cárdenas, Soledad; Valcárcel, Miguel

    2007-05-01

    On many occasions, sample treatment is a critical step in electrophoretic analysis. As an alternative to batch procedures, in this work, a new strategy is presented with a view to develop an on-capillary sample cleanup method. This strategy is based on the partial filling of the capillary with carboxylated single-walled carbon nanotube (c-SWNT). The nanoparticles retain interferences from the matrix allowing the determination and quantification of carbohydrates (viz glucose, maltose and fructose). The precision of the method for the analysis of real samples ranged from 5.3 to 6.4%. The proposed method was compared with a method based on a batch filtration of the juice sample through diatomaceous earth and further electrophoretic determination. This method was also validated in this work. The RSD for this other method ranged from 5.1 to 6%. The results obtained by both methods were statistically comparable demonstrating the accuracy of the proposed methods and their effectiveness. Electrophoretic separation of carbohydrates was achieved using 200 mM borate solution as a buffer at pH 9.5 and applying 15 kV. During separation, the capillary temperature was kept constant at 40 degrees C. For the on-capillary cleanup method, a solution containing 50 mg/L of c-SWNTs prepared in 300 mM borate solution at pH 9.5 was introduced for 60 s into the capillary just before sample introduction. For the electrophoretic analysis of samples cleaned in batch with diatomaceous earth, it is also recommended to introduce into the capillary, just before the sample, a 300 mM borate solution as it enhances the sensitivity and electrophoretic resolution.

  20. Separation of carbohydrates using hydrophilic interaction liquid chromatography.

    Science.gov (United States)

    Fu, Qing; Liang, Tu; Li, Zhenyu; Xu, Xiaoyong; Ke, Yanxiong; Jin, Yu; Liang, Xinmiao

    2013-09-20

    A strategy was developed to rapidly evaluate chromatographic properties of hydrophilic interaction chromatography (HILIC) columns for separating carbohydrates. Seven HILIC columns (Silica, Diol, TSK Amide-80, XAmide, Click Maltose, Click β-CD, and Click TE-Cys columns) were evaluated by using three monosaccharide and seven disaccharides as probes. The influence of column temperature on the peak shape and tautomerization of carbohydrates, as well as column selectivity were investigated. The influence of surface charge property on the retention was also studied by using glucose, glucuronic acid, and glucosamine, which indicated that buffer salt concentration and pH value in mobile phase was necessary to control the ionic interactions between ionic carbohydrates and HILIC columns. According to evaluation results, the XAmide column was selected as an example to establish experimental schemes for separation of complex mixtures of oligosaccharide.