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Sample records for capacidad contractil uterina

  1. Observations of motor reactions to ions, hormones, pharmaceuticals, electrical stimulation and X irradiation in intraoperatively removed human genital preparations (myometrium, tuba uterina and genital vessels)

    International Nuclear Information System (INIS)

    Elementary physiological, pharmacological and radiological characteristics of isolated genital preparations - myometrium, tuba uterina and genital vessels - from human op material were compared and analysed. The basal tone and the spontaneous contractile activity were dependent on the sodium, potassium and calcium chloride concentration in the nutritive solution. Observations of the motor reactions of the preparations of myometrium and tuba uterina to hormones and their antagonists confirm or indicate the presence of specific hormone receptors. X-radiation caused a tonic contraction and changes in the spontaneous contractile activity in myometrium preparations. Uterine and ovarian vessels reacted to irradiated with definite and often enduring tonic contractions. Theophyllin, a substance with an inhibiting affect on phosphodiesterase, which leads to an increase in intracellular cAMP concentration, works against the X-radiation influence. (MBC)

  2. Myocardial contractility

    Energy Technology Data Exchange (ETDEWEB)

    Comet, M.; Machecourt, J.

    1988-01-01

    The myocardial contractility characterizes the intensity of the activation representing the globality of the processes which lead to the formation of the sites where the strength is generated. Some parameters allowing a quantification of the contractility are measured during the isovolumic phase of the ventricular contraction: they are essentially dP/dt/sub max/, and eventually V/sub max/. For the measurement of these parameters, a pressure detector needs to be introduced into the left ventricle. Other parameters are measured during the systolic ejection phase: they are the ejection fraction, which is easy to measure and is very load dependant, and the maximal elastance. This last parameter is not easy to measure, but seems load independant. With the radioisotopes, it is possible to measure the ejection fraction and the maximal elastance. Contractility measurements are of interest in the study of ischemic cardiopathies and of valvular desease.

  3. Altas capacidades e interdisciplinariedad

    OpenAIRE

    Moreno-García, María José

    2013-01-01

    El presente trabajo fin de grado trata de abordar la importancia de dar una respuesta educativa acorde a las necesidades del alumnado de altas capacidades. Para ello, primeramente, se ha visto necesario definir el término de altas capacidades, analizar sus características, matizar la importancia de su detección temprana, desbancar falsos mitos y conocer la legislación vigente al respecto, para posteriormente elaborar una propuesta de intervención, que se ha llevado a cabo en el Colegio Nelva,...

  4. Fractalkine depresses cardiomyocyte contractility.

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    David Taube

    Full Text Available BACKGROUND: Our laboratory reported that male mice with cardiomyocyte-selective knockout of the prostaglandin E2 EP4 receptor sub-type (EP4 KO exhibit reduced cardiac function. Gene array on left ventricles (LV showed increased fractalkine, a chemokine implicated in heart failure. We therefore hypothesized that fractalkine is regulated by PGE2 and contributes to depressed contractility via alterations in intracellular calcium. METHODS: Fractalkine was measured in LV of 28-32 week old male EP4 KO and wild type controls (WT by ELISA and the effect of PGE2 on fractalkine secretion was measured in cultured neonatal cardiomyocytes and fibroblasts. The effect of fractalkine on contractility and intracellular calcium was determined in Fura-2 AM-loaded, electrical field-paced cardiomyocytes. Cardiomyocytes (AVM from male C57Bl/6 mice were treated with fractalkine and responses measured under basal conditions and after isoproterenol (Iso stimulation. RESULTS: LV fractalkine was increased in EP4 KO mice but surprisingly, PGE2 regulated fractalkine secretion only in fibroblasts. Fractalkine treatment of AVM decreased both the speed of contraction and relaxation under basal conditions and after Iso stimulation. Despite reducing contractility after Iso stimulation, fractalkine increased the Ca(2+ transient amplitude but decreased phosphorylation of cardiac troponin I, suggesting direct effects on the contractile machinery. CONCLUSIONS: Fractalkine depresses myocyte contractility by mechanisms downstream of intracellular calcium.

  5. Symphysis-fundal height curve in the diagnosis of fetal growth deviations Curva de altura uterina por edad de gestación y diagnóstico de desvíos del crecimiento fetal Curva de altura uterina por idade gestacional e diagnóstico de desvios do crescimento fetal

    Directory of Open Access Journals (Sweden)

    Djacyr Magna Cabral Freire

    2010-12-01

    marzo y octubre de 2006, con edad de gestación por encima de 27 semanas. La altura uterina fue medida de acuerdo con técnica recomendada por el Ministerio de la Salud. El patrón-oro fue el peso fetal, estimado por el ultrasonido con base en la curva de referencia brasilera por edad de gestación. Una sub-muestra de 122 casos con pesos neonatales obtenidos hasta siete días después de la estimación del peso fetal, la clasificación de la altura uterina fue comparada con la curva de Lubchenco como estándar-oro. La sensibilidad, la especificidad y los valores predictivos positivo y negativo fueron calculados. Para comparar el desempeño de la sensibilidad entre ambas curvas de altura uterina, se utilizó la prueba chi-cuadrado de McNemar. RESULTADOS: La sensibilidad de la nueva curva para la detección de fetos pequeños para la edad de gestación fue de 51,6%, mientras que la curva del patrón-oro fue significativamente menor (12,5%. En la sub-muestra que tuvo el peso neonatal como estándar-oro, la sensibilidad de la nueva curva de referencia fue de 87,7%, mientras que la del Ministerio de la Salud exhibió 42,9% de sensibilidad para la detección de fetos pequeños para la edad de gestación. CONCLUSIONES: La capacidad diagnóstica de la nueva curva de referencia para detectar fetos pequeños para la edad de gestación fue significativamente mejor que la curva recomendada por el Ministerio de la Salud.OBJETIVO: Validar curva de referência de altura uterina por idade gestacional para o rastreamento de desvios do crescimento fetal e comparar sua performance com a curva-padrão adotada pelo Ministério da Saúde do Brasil. MÉTODOS: Estudo observacional que envolveu 753 gestantes de baixo risco de João Pessoa, PB, entre março e outubro de 2006, com idade gestacional acima de 27 semanas. A altura uterina foi medida de acordo com técnica preconizada pelo Ministério da Saúde. O padrão-ouro foi o peso fetal, estimado pelo ultrassom com base na curva de refer

  6. Ruptura Uterina - A Propósito de 2 Casos Clínicos

    OpenAIRE

    Olival, V; Condeço, R; Vaz, D; Sousa, F; Nunes, MJ; C. Leitão; Ferreira, C.; Caetano, M.; Dias, I.; Coelho, M; Mira, R

    2011-01-01

    Introdução: A ruptura uterina leva a consequências graves materno-fetais. A maioria dos casos ocorre em grávidas com cesarianas anteriores ou incisões uterinas prévias como miomectomia, raramente ocorrendo em úteros sem cicatrizes. Um dos principais factores correlacionado com o risco de ruptura é o tipo de incisão da histerotomia prévia: clássica (4-9%), em T (4-9%), vertical (1-7%); transversa (0,2-1,5%). Outros factores de risco são: ausência de parto vaginal anterior, indução do trabalho ...

  7. Avaliação da Cavidade Uterina: Estudo Comparativo entre Histerografia, Histerossonografia e Histeroscopia

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    Franco Rodrigo Coelho

    2000-01-01

    Full Text Available Objetivo: realizar um estudo comparativo entre os métodos utilizados para avaliar a cavidade uterina por meio da análise da sensibilidade e especificidade da histerografia e da histerossonografia, comparadas com a histeroscopia (padrão ouro. Métodos: foi realizado um estudo prospectivo para a investigação da cavidade uterina com a histerografia, a histerossonografia e a histeroscopia em 18 pacientes. Todas as pacientes apresentavam suspeita de anomalias da cavidade uterina. As principais indicações para a investigação foram: distúrbios do ciclo menstrual, sangramento pós-menopausa e alterações observadas em exames ultra-sonográficos sugestivas de pólipos, miomas e/ou espessamento endometrial. Resultados: a sensibilidade da histerossonografia foi de 90% e a especificidade 87,5%, quando comparadas à histeroscopia. O valor preditivo positivo foi de 90% e o negativo 87,5%. A histerografia, por sua vez, apresentou sensibilidade de 70%, especificidade de 75%, valor preditivo positivo de 77,7% e valor preditivo negativo de 75%. Conclusão: a histerossonografia parece ser um bom método alternativo para a avaliação da cavidade uterina, identificando algumas lesões não observadas à ultra-sonografia convencional ou à histerografia, e permitindo a triagem das pacientes candidatas à realização de uma histeroscopia diagnóstica ou cirúrgica. A histerografia apresentou resultados inferiores à histerossonografia, e não parece ser de utilidade na triagem das pacientes.

  8. Tratamento dos Leiomiomas por Embolização das Artérias Uterinas

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    Messina Marcos de Lorenzo

    2001-01-01

    Full Text Available Objetivos: avaliar os resultados da embolização das artérias uterinas (EAU como tratamento do leiomioma uterino. Métodos: foram estudadas 18 mulheres com diagnóstico ultra-sonográfico de leiomioma uterino submetidas à EAU com partículas de álcool polivinílico (PVA. O acesso arterial foi realizado por punção da artéria femoral e a progressão do cateter foi monitorada até atingir seletivamente as artérias uterinas. A avaliação dos resultados foi realizada pelo estudo ultra-sonográfico antes e três meses após o procedimento. Foi realizado controle clínico evolutivo para avaliação das características menstruais e do volume uterino. Resultados: houve insucesso técnico em três casos por dificuldade de cateterização das artérias uterinas. O sangramento e a dismenorréia foram controlados no intervalo de três meses em 86 e 60% das pacientes, respectivamente. A média dos volumes uterinos iniciais foi de 381 cm³ e após 3 meses foi de 263 cm³. Houve redução média de 27,4% no volume uterino três meses após o tratamento. Três pacientes tiveram insuficiência ovariana clínica e laboratorial (20% dos casos. Conclusões: a EAU representa alternativa terapêutica para o tratamento dos leiomiomas uterinos. Em virtude do risco de insuficiência ovariana esse procedimento é reservado para pacientes com mais de 45 anos de idade ou com prole completa.

  9. A Histerossonografia na Avaliação da Cavidade Uterina em Pacientes Menopausadas

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    Ceccato Júnior Benito Pio Vitório

    2002-01-01

    Full Text Available Objetivos: avaliar a acuidade diagnóstica da histerossonografia como método de avaliação da cavidade uterina em pacientes menopausadas com cavidade uterina alterada à ultra-sonografia endovaginal convencional. Métodos: este estudo consistiu na avaliação de 99 pacientes menopausadas com cavidade uterina anormal à ultra-sonografia endovaginal convencional, caracterizada por espessura endometrial maior ou igual a 5 mm em pacientes sem terapia de reposição hormonal, ou espessura endometrial maior ou igual a 8 mm em pacientes em terapia de reposição hormonal, com sangramento irregular. Estas pacientes foram submetidas à histerossonografia e após, foram obtidas amostras para avaliação histopatológica por biópsia dirigida por histeroscopia em 92 pacientes, biópsia endometrial em quatro pacientes e histerectomia em três pacientes. Os resultados da histerossonografia foram comparados com os resultados do exame histopatológico, considerado como "padrão-ouro". Resultados: houve oito casos de cavidade uterina normal e 20 casos de atrofia endometrial e a histerossonografia teve altos níveis de especificidade (97,8 e 97,5% e baixa sensibilidade (35 e 25%. Houve altos níveis de sensibilidade (92,3 e 75,0% e especificidade (94,1 e 97,9% em pólipos (65 casos e miomas submucosos (quatro casos. Houve três casos de câncer de endométrio e a histerossonografia teve sensibilidade e especificidade de 100%. Conclusões: a histerossonografia mostrou boa acuidade no diagnóstico de doenças focais (pólipos endometriais e miomas submucosos, com altos níveis de sensibilidade e especificidade. Houve três casos de câncer endometrial, e a histerossonografia diagnosticou corretamente todos eles. Mostrou também ser método acurado para excluir anormalidades endometriais. Entretanto, nos casos de espessamento endometrial difuso, a acuidade é baixa, porque endométrios atróficos ou normais freqüentemente aparecem como tendo espessamento difuso

  10. Capital humano y capacidad humana

    OpenAIRE

    Sen, Amartya Kumar

    2010-01-01

    En este articulo se examinan las relaciones y diferencias entre el concepto de 'capital humano' y el concepto de 'capacidad humana'. El concepto de capital humano es mas limitado puesto que solo concibe las cualidades humanas en su relación con el crecimiento económico mientras que el concepto de capacidades da énfasis a la expansión de libertad humana para vivir el tipo de vida que la gente considera valedera. Cuando se adopta esa visión mas amplia, el proceso de desarrollo no puede verse si...

  11. Structural comparison of contractile nanomachines

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    Sebastian Kube

    2015-05-01

    Full Text Available Contractile molecular machines are a common feature among bacteriophages and prokaryotes. Due to their stability and the large size, contractile-tailed bacteriophages are traditionally investigated by electron microscopic methods. Complemented by crystallographic studies, a molecular model of contraction for the T4 phage was developed. Lately, also related contractile structures like the Photorhabdus virulence cassette-like particles, the R-Type pyocins and the contractile tubule of the bacterial Type VI secretion system have been analyzed by cryo electron microscopy. Photorhabdus virulence cassette particles and R-Type pyocins are toxin complexes reminiscent of bacteriophage tails that are secreted by bacteria to kill their insect host or competing bacteria. In contrast, the Type VI secretion system is an intracellular apparatus for injection of effector proteins into bacterial and eukaryotic cells. Although it shares homology with other contractile systems, the Type VI secretion system is additionally equipped with a recycling function, which makes it suitable for multiple rounds of action. Starting from the 3D reconstructions, we compare these molecular machines structurally and functionally to their viral counterparts and summarize the current knowledge on their respective mode of action.

  12. Doppler de arterias uterinas en semana 11 – 13 6/7 como predictor de desenlaces materno - perinatales adversos

    OpenAIRE

    Mejía Pérez, Dauris Lineth; Olaya Trujillo, Diego Alejandro

    2016-01-01

    Este es un estudio de Cohorte prospectiva longitudinal, realizado en una población de mujeres gestantes de bajo riesgo. Se incluyeron pacientes con posibilidad de seguimiento durante 18 meses, se reclutaron entre la semanas 11 a 13 6/7, Se hizo medición de índices de pulsatilidad y resistencia de las arterias uterinas derecha e izquierda. Se determinaron por seguimiento e historia clínica los siguientes desenlaces maternos perinatales: trastornos hipertensivos asociados al e...

  13. Modulatory effects of taurine on jejunal contractility

    OpenAIRE

    Yao, Q Y; Chen, D. P.; D.M. Ye; Y.P. Diao; Lin, Y.

    2014-01-01

    Taurine (2-aminoethanesulfonic acid) is widely distributed in animal tissues and has diverse pharmacological effects. However, the role of taurine in modulating smooth muscle contractility is still controversial. We propose that taurine (5-80 mM) can exert bidirectional modulation on the contractility of isolated rat jejunal segments. Different low and high contractile states were induced in isolated jejunal segments of rats to observe the effects of taurine and the associated mechanisms. Tau...

  14. Uso de nifedipina en el manejo de la hiperestimulación uterina producida por misoprostol.

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    Rodrigo Cifuentes

    2009-11-01

    Full Text Available La medicina basada en la evidencia ha demostrado mediante una serie de estudios, la eficacia clínica de algunos fármacos, como el caso del misoprostol análogo de la prostaglandina E1 utilizado en obstetricia para lograr maduración cervical en inducción de trabajo de parto con cérvix no favorable, con un efecto no deseado en la mayoría de los casos como lo es la hiperestimulación uterina. Se diseñó un estudio clínico prospectivo entre noviembre, 2002 y febrero, 2003 en la Clínica Rafael Uribe Uribe del Instituto de Seguro Social, Cali, donde se incluyeron 29 pacientes con criterio para inducción del trabajo de parto con índice de Bishop no favorable; se les administró 50 mcg de misoprostol por vía vaginal y desarrollaron polisistolia (>5 contracciones en 10 minutos. A estas 29 pacientes se les suministró 20 mg de nifedipina por vía oral en el momento en que apareció la polisistolia y se estableció mediante el registro por monitoría electrónica maternofetal durante 30 minutos la disminución en el número, la duración de las contracciones y la “actividad uterina”.

  15. Modulatory effects of taurine on jejunal contractility

    Directory of Open Access Journals (Sweden)

    Q.Y. Yao

    2014-12-01

    Full Text Available Taurine (2-aminoethanesulfonic acid is widely distributed in animal tissues and has diverse pharmacological effects. However, the role of taurine in modulating smooth muscle contractility is still controversial. We propose that taurine (5-80 mM can exert bidirectional modulation on the contractility of isolated rat jejunal segments. Different low and high contractile states were induced in isolated jejunal segments of rats to observe the effects of taurine and the associated mechanisms. Taurine induced stimulatory effects on the contractility of isolated rat jejunal segments at 3 different low contractile states, and inhibitory effects at 3 different high contractile states. Bidirectional modulation was not observed in the presence of verapamil or tetrodotoxin, suggesting that taurine-induced bidirectional modulation is Ca2+ dependent and requires the presence of the enteric nervous system. The stimulatory effects of taurine on the contractility of isolated jejunal segments was blocked by atropine but not by diphenhydramine or by cimetidine, suggesting that muscarinic-linked activation was involved in the stimulatory effects when isolated jejunal segments were in a low contractile state. The inhibitory effects of taurine on the contractility of isolated jejunal segments were blocked by propranolol and L-NG-nitroarginine but not by phentolamine, suggesting that adrenergic β receptors and a nitric oxide relaxing mechanism were involved when isolated jejunal segments were in high contractile states. No bidirectional effects of taurine on myosin phosphorylation were observed. The contractile states of jejunal segments determine taurine-induced stimulatory or inhibitory effects, which are associated with muscarinic receptors and adrenergic β receptors, and a nitric oxide associated relaxing mechanism.

  16. Modulatory effects of taurine on jejunal contractility

    Energy Technology Data Exchange (ETDEWEB)

    Yao, Q.Y.; Chen, D.P.; Ye, D.M.; Diao, Y.P.; Lin, Y. [Dalian Medical University, Dalian, Liaoning (China)

    2014-10-14

    Taurine (2-aminoethanesulfonic acid) is widely distributed in animal tissues and has diverse pharmacological effects. However, the role of taurine in modulating smooth muscle contractility is still controversial. We propose that taurine (5-80 mM) can exert bidirectional modulation on the contractility of isolated rat jejunal segments. Different low and high contractile states were induced in isolated jejunal segments of rats to observe the effects of taurine and the associated mechanisms. Taurine induced stimulatory effects on the contractility of isolated rat jejunal segments at 3 different low contractile states, and inhibitory effects at 3 different high contractile states. Bidirectional modulation was not observed in the presence of verapamil or tetrodotoxin, suggesting that taurine-induced bidirectional modulation is Ca{sup 2+} dependent and requires the presence of the enteric nervous system. The stimulatory effects of taurine on the contractility of isolated jejunal segments was blocked by atropine but not by diphenhydramine or by cimetidine, suggesting that muscarinic-linked activation was involved in the stimulatory effects when isolated jejunal segments were in a low contractile state. The inhibitory effects of taurine on the contractility of isolated jejunal segments were blocked by propranolol and L-NG-nitroarginine but not by phentolamine, suggesting that adrenergic β receptors and a nitric oxide relaxing mechanism were involved when isolated jejunal segments were in high contractile states. No bidirectional effects of taurine on myosin phosphorylation were observed. The contractile states of jejunal segments determine taurine-induced stimulatory or inhibitory effects, which are associated with muscarinic receptors and adrenergic β receptors, and a nitric oxide associated relaxing mechanism.

  17. Modulatory effects of taurine on jejunal contractility

    International Nuclear Information System (INIS)

    Taurine (2-aminoethanesulfonic acid) is widely distributed in animal tissues and has diverse pharmacological effects. However, the role of taurine in modulating smooth muscle contractility is still controversial. We propose that taurine (5-80 mM) can exert bidirectional modulation on the contractility of isolated rat jejunal segments. Different low and high contractile states were induced in isolated jejunal segments of rats to observe the effects of taurine and the associated mechanisms. Taurine induced stimulatory effects on the contractility of isolated rat jejunal segments at 3 different low contractile states, and inhibitory effects at 3 different high contractile states. Bidirectional modulation was not observed in the presence of verapamil or tetrodotoxin, suggesting that taurine-induced bidirectional modulation is Ca2+ dependent and requires the presence of the enteric nervous system. The stimulatory effects of taurine on the contractility of isolated jejunal segments was blocked by atropine but not by diphenhydramine or by cimetidine, suggesting that muscarinic-linked activation was involved in the stimulatory effects when isolated jejunal segments were in a low contractile state. The inhibitory effects of taurine on the contractility of isolated jejunal segments were blocked by propranolol and L-NG-nitroarginine but not by phentolamine, suggesting that adrenergic β receptors and a nitric oxide relaxing mechanism were involved when isolated jejunal segments were in high contractile states. No bidirectional effects of taurine on myosin phosphorylation were observed. The contractile states of jejunal segments determine taurine-induced stimulatory or inhibitory effects, which are associated with muscarinic receptors and adrenergic β receptors, and a nitric oxide associated relaxing mechanism

  18. Capacidade de aprendizagem das empresas familiares

    OpenAIRE

    Fernandes, António B.; Ussmane, Ana Maria

    2012-01-01

    A capacidade de aprender de uma empresa é frequentemente apontada como uma estratégia viável para a sua longevidade. Esta capacidade tem maior impacto nas empresas familiares, dadas as suas particularidades. Assim, pretende-se com o presente trabalho analisar a capacidade de aprender de um conjunto de empresas seleccionadas aleatoriamente. Para tal, após uma revisão da literatura, procede-se a um estudo empírico, com base em dados primários recolhidos de um estudo de casos múltiplos de 22 ...

  19. Distribuição da altura uterina ao longo da gestação em uma coorte brasileira: comparação com a curva de referência do Centro Latino-Americano de Perinatologia Distribution of uterine height during pregnancy in a Brazilian cohort: comparison with the reference curve of the Centro Latino-Americano de Perinatologia

    Directory of Open Access Journals (Sweden)

    Maria Lúcia Rocha Oppermann

    2006-09-01

    Full Text Available OBJETIVOS: descrever, em gestantes do Estudo Brasileiro do Diabetes Gestacional (EBDG, a distribuição da altura uterina de acordo com a idade gestacional e validar a curva do Centro Latino-Americano de Perinatologia (CLAP, curva de referência para a predição de anormalidades do crescimento fetal. MÉTODOS: o EBDG é uma coorte de 5564 gestantes, com mais de 19 anos, seguidas até e após o parto. Entrevistas e medidas antropométricas padronizadas foram feitas no arrolamento entre a 20ª e a 28ª semana. Os prontuários foram revisados segundo protocolo padronizado, abrangendo os períodos de pré-natal e parto. As análises referem-se a 3539 gestantes com datação da gravidez confirmada por ultra-sonografia. Determinamos as propriedades diagnósticas dos percentis 10 e 90 de altura uterina de ambas as curvas (EBDG e CLAP como indicadores de anormalidade no peso neonatal. RESULTADOS: as medidas de altura uterina no EBDG foram maiores que as do CLAP em todas as semanas de gestação (1 a 4 cm e 2 a 6 cm, respectivamente, nos percentis 10 e 90. O percentil 10 do CLAP identificou como pequenas as medidas uterinas de 0,3 a 1,7% das gestantes brasileiras, ao passo que o percentil 90 classificou como grandes as medidas uterinas de 42 a 57% das brasileiras. A sensibilidade do percentil 10 do CLAP para predizer recém-nascidos pequenos para a idade gestacional variou de 0,8 a 6% e a especificidade do percentil 90 para predizer grandes para a idade gestacional, de 46 a 61%. CONCLUSÕES: a curva de referência do CLAP não reflete o padrão de crescimento uterino das gestantes brasileiras, limitando sua capacidade de identificar anormalidades de crescimento fetal, especialmente a restrição de crescimento.PURPOSE: to describe, in participants of the Brazilian Study of Gestational Diabetes (EBDG, the percentile distribution of uterine height by gestational age and to validate the use of percentiles of the chart derived by the "Centro Latino

  20. Compensatory Hypertrophy of Skeletal Muscle: Contractile Characteristics

    Science.gov (United States)

    Ianuzzo, C. D.; Chen, V.

    1977-01-01

    Describes an experiment using rats that demonstrates contractile characteristics of normal and hypertrophied muscle. Compensatory hypertrophy of the plantaris muscle is induced by surgical removal of the synergistic gastrocnemium muscle. Includes methods for determination of contractile properties of normal and hypertrophied muscle and…

  1. Frequência de mola hidatiforme em tecidos obtidos por curetagem uterina Frequency of hydatidiform mole in tissue obtained by curettage

    OpenAIRE

    Andressa Biscaro; Sheila Koettker Silveira; Giovani de Figueiredo Locks; Lívia Ribeiro Mileo; João Péricles da Silva Júnior; Péricles Pretto

    2012-01-01

    OBJETIVO: Determinar a frequência de mola hidatiforme em tecidos obtidos por curetagem uterina. MÉTODOS: Estudo transversal, prospectivo e descritivo que incluiu pacientes submetidas à curetagem uterina por diagnóstico de aborto ou mola hidatiforme cujo material obtido foi encaminhado para exame anatomopatológico. Foram excluídas aquelas que não aceitaram participar da pesquisa, recusando-se a assinar o Termo de Consentimento Informado Livre e Esclarecido. Foram analisadas as seguintes variáv...

  2. Factor de crecimiento vascular endotelial total y Doppler de las arterias uterinas en preeclampticas y embarazadas normotensas sanas

    OpenAIRE

    Eduardo Reyna-Villasmil; Jorly Mejia-Montilla; Joel Santos-Bolívar; Duly Torres-Cepeda; Yolimar Navarro-Briceño; Nadia Reyna-Villasmil; Ismael Suarez-Torres

    2014-01-01

    El objetivo de la investigación fue establecer las concentraciones de factor de crecimiento vascular endotelial (VEGF) total en preeclámpticas y embarazadas normotensas, y relacionar los valores de los hallazgos Doppler de las arterias uterinas con las concentraciones plasmáticas. Se seleccionaron 160 sujetos. Se incluyeron 47 preeclámpticas severas (grupo A), 33 preeclámpticas leves (grupo B) y un grupo control con edades similares a los grupos de estudio de 80 embarazadas sanas (grupo C). L...

  3. Cardiotocógrafo: monitor para el control de la frecuencia cardiaca fetal y la dinámica uterina

    OpenAIRE

    Gómez, Carmen; Terré Rull, Carmen

    1991-01-01

    Controlar la frecuencia cardiaca fetal y la dinámica uterina durante el proceso del embarazo y el parto resulta de particular importancia para conocer el estado de salud de la madre y el niño. Hoy en día existen aparatos muy novedosos para este fin de uso generalizado en los hospitales españoles. De este tema se ocupa la Ficha de Utillaje de este mes analizando en qué consiste este monitor; cómo se utiliza, los pasos a seguir; etc.

  4. Cellular contractility requires ubiquitin mediated proteolysis.

    Directory of Open Access Journals (Sweden)

    Yuval Cinnamon

    Full Text Available BACKGROUND: Cellular contractility, essential for cell movement and proliferation, is regulated by microtubules, RhoA and actomyosin. The RhoA dependent kinase ROCK ensures the phosphorylation of the regulatory Myosin II Light Chain (MLC Ser19, thereby activating actomyosin contractions. Microtubules are upstream inhibitors of contractility and their depolymerization or depletion cause cells to contract by activating RhoA. How microtubule dynamics regulates RhoA remains, a major missing link in understanding contractility. PRINCIPAL FINDINGS: We observed that contractility is inhibited by microtubules not only, as previously reported, in adherent cells, but also in non-adhering interphase and mitotic cells. Strikingly we observed that contractility requires ubiquitin mediated proteolysis by a Cullin-RING ubiquitin ligase. Inhibition of proteolysis, ubiquitination and neddylation all led to complete cessation of contractility and considerably reduced MLC Ser19 phosphorylation. CONCLUSIONS: Our results imply that cells express a contractility inhibitor that is degraded by ubiquitin mediated proteolysis, either constitutively or in response to microtubule depolymerization. This degradation seems to depend on a Cullin-RING ubiquitin ligase and is required for cellular contractions.

  5. LA CAPACIDAD ACELERATIVA EN EL DEPORTE

    OpenAIRE

    Isidoro Hornillos Baz

    2010-01-01

    La capacidad acelerativa adquiere una gran relevancia en las disciplinas deportivas que exigen desplazamientos rápidos. Forma parte de la velocidad, como cualidad motora, en su dimensión compleja. Desde una perspectiva bioenergética, la aceleración depende de la potencia del sistema anaeróbico aláctico. Para mejorar esta capacidad será necesario realizar ejercicios a la máxima intensidad, con recuperaciones completas y con una duración no superior a los seis segundos, en general. También desa...

  6. Estudo da involução uterina por meio da ultra-sonografia (modo-B) em cadelas submetidas a cesariana

    OpenAIRE

    Ferri S.T.S.; Vicente W.R.R.; Toniollo G.H.

    2003-01-01

    Utilizaram-se 15 cadelas gestantes submetidas a cesariana e posteriormente a exames ultra-sonográficos seriados, em modo B (tempo real), para averiguação do diâmetro uterino nos dias 0, 3, 7, 14 e 21 pós-cesariana, com o objetivo de verificar a influência do ato cirúrgico na involução uterina. Os resultados (média e desvio-padrão, em centímetros) registrados para os dias estudados foram, respectivamente: 3,99+0,71; 3,27+0,51; 2,60+0,54; 2,01+0,34; 1,28+0,24. A involução uterina pós-cesariana ...

  7. LA CAPACIDAD ACELERATIVA EN EL DEPORTE

    Directory of Open Access Journals (Sweden)

    Isidoro Hornillos Baz

    2010-01-01

    Full Text Available La capacidad acelerativa adquiere una gran relevancia en las disciplinas deportivas que exigen desplazamientos rápidos. Forma parte de la velocidad, como cualidad motora, en su dimensión compleja. Desde una perspectiva bioenergética, la aceleración depende de la potencia del sistema anaeróbico aláctico. Para mejorar esta capacidad será necesario realizar ejercicios a la máxima intensidad, con recuperaciones completas y con una duración no superior a los seis segundos, en general. También desarrollar las técnicas de salida y de carrera. Los principales medios para la mejora de la aceleración son los multisaltos; las cuestas; los arrastres; los ejercicios con cinturones y tobilleras; los lastres en forma de cinturones, tobilleras o carreras con paracaídas; el entrenamiento de fuerza con cargas y los sprints en terreno liso. Pero en algunos deportes colectivos va a ser determinante la resistencia a la aceleración, debido al alto número de carreras de máxima o alta velocidad durante un partido. En estos casos será también necesario desarrollar la capacidad anaeróbica aláctica y la potencia y capacidad lactácida. Asimismo, hay que alcanzar un mínimo nivel de potencia aeróbica, que representa la máxima cantidad de producción de ATP por unidad de tiempo en base a procesos metabólicos de naturaleza aeróbica.

  8. Rotura Uterina Espontánea en embarazo de segundo trimestre: presentación de un caso y revisión de la literatura

    Directory of Open Access Journals (Sweden)

    Airam Amoroso

    2013-09-01

    Full Text Available La rotura uterina se define como la solución de continuidad de la pared uterina. Es una complicación obstétrica poco frecuente y potencialmente letal para la madre y el feto, que ocurre generalmente durante el segundo o tercer trimestre de gestación, y que está asociada principalmente a la cirugía uterina previa, constituyendo así un desafío médico por su difícil diagnóstico diferencial y controversial manejo. Se presenta el caso de una paciente de 26 años de edad, con rotura espontánea de útero grávido de 18 semanas de gestación y antecedente de cicatriz uterina. Se realiza laparotomía exploradora obteniendo como hallazgo placenta y cordón umbilical unido a feto sin vida en cavidad abdominal, lo que confirma diagnóstico intraoperatorio. Spontaneous Uterine Rupture in Second Trimester of Pregnancy: Case report and Literature review Abstract Uterine rupture is a solution of continuity of the uterine wall. It is an uncommon and potentially lethal obstetric complication, for both mother and fetus, that mainly occurs during the second or third trimester of pregnancy, and that is mainly associated to previous uterine surgery, thus forming a medical challenge for its difficult differential diagnosis and controversial management. We report the case of a 26 years old patient with a spontaneous gravid uterine rupture, 18 weeks of gestation and cesarean scar background. Laparotomy is conducted finding both placenta and umbilical cord attached to no-living fetus in the abdominal cavity, which confirms intraoperative diagnosis.

  9. Estudo da involução uterina por meio da ultra-sonografia (modo-B em cadelas submetidas a cesariana

    Directory of Open Access Journals (Sweden)

    Ferri S.T.S.

    2003-01-01

    Full Text Available Utilizaram-se 15 cadelas gestantes submetidas a cesariana e posteriormente a exames ultra-sonográficos seriados, em modo B (tempo real, para averiguação do diâmetro uterino nos dias 0, 3, 7, 14 e 21 pós-cesariana, com o objetivo de verificar a influência do ato cirúrgico na involução uterina. Os resultados (média e desvio-padrão, em centímetros registrados para os dias estudados foram, respectivamente: 3,99+0,71; 3,27+0,51; 2,60+0,54; 2,01+0,34; 1,28+0,24. A involução uterina pós-cesariana seguiu o mesmo padrão do puerpério do parto normal, assim como as características das imagens ultra-sonográficas. O parto cesariana não influenciou no padrão de involução uterina.

  10. Architecture and Connectivity Govern Actin Network Contractility.

    Science.gov (United States)

    Ennomani, Hajer; Letort, Gaëlle; Guérin, Christophe; Martiel, Jean-Louis; Cao, Wenxiang; Nédélec, François; De La Cruz, Enrique M; Théry, Manuel; Blanchoin, Laurent

    2016-03-01

    Actomyosin contractility plays a central role in a wide range of cellular processes, including the establishment of cell polarity, cell migration, tissue integrity, and morphogenesis during development. The contractile response is variable and depends on actomyosin network architecture and biochemical composition. To determine how this coupling regulates actomyosin-driven contraction, we used a micropatterning method that enables the spatial control of actin assembly. We generated a variety of actin templates and measured how defined actin structures respond to myosin-induced forces. We found that the same actin filament crosslinkers either enhance or inhibit the contractility of a network, depending on the organization of actin within the network. Numerical simulations unified the roles of actin filament branching and crosslinking during actomyosin contraction. Specifically, we introduce the concept of "network connectivity" and show that the contractions of distinct actin architectures are described by the same master curve when considering their degree of connectivity. This makes it possible to predict the dynamic response of defined actin structures to transient changes in connectivity. We propose that, depending on the connectivity and the architecture, network contraction is dominated by either sarcomeric-like or buckling mechanisms. More generally, this study reveals how actin network contractility depends on its architecture under a defined set of biochemical conditions. PMID:26898468

  11. Carcinoma urotelial primario de la trompa uterina, una patología infrecuente: reporte de un caso

    Directory of Open Access Journals (Sweden)

    Stefano Pozzobon-Borregales

    2013-01-01

    Full Text Available El carcinoma primario de la trompa uterina es una patología maligna infrecuente que ocurre entre el 0,1% al 1,8% de todos los tumores malignos del organismo, siendo aun menos frecuente el tipo histológico transicional o urotelial que representa el 10% de los tumores malignos de trompa uterina. Se presentan principalmente entre la 5ta y 7ma décadas de la vida, y clínicamente se manifiesta, en el 18% de los casos, con la triada de masa palpable y/o distensión abdominal, dolor pélvico y metrorragia. Presentamos el caso de una paciente de 44 años de edad quien inicia enfermedad actual en diciembre del 2010, con antecedente de dolor pélvico y sangrado genital continuo. En la resonancia magnética se apreció un lesión ocupante de espacio parauterina derecha, sugestiva de neoplasia maligna de ovario derecho. El reporte del marcador tumoral Ca 125 mostró valores elevados, acompañado de clínica y paraclínica sugestiva enfermedad tumoral maligna. Se decidió realizar laparotomía ginecológica en la que se evidenció tumoración en trompa uterina derecha. El estudio histológico se concluyó como carcinoma con diferenciación uroterial. Posteriormente se realizó cirugía de estadiaje, que incluyó lavado peritoneal, histerectomía total, salpingooforectomía izquierda, omentectomía subcolónica, apendicectomía y linfadenectomía bilateral selectiva. El reporte de biopsia de dichas piezas operatorias resultaron negativas para malignidad. La paciente se encuentra actualmente libre de enfermedad. Dado lo infrecuente de la patología se reporta este caso. Primary Carcinoma of the uterine tube, an unusual malignant pathology: case report Abstract Primary Carcinoma of the uterine tube is an unusual malignant pathology that occurs between the 0,1% and the 1,8% of all malignant tumors, being the urothelial histological type even less frequent, which represents 10% of malignant tumors of the uterine tubes. These tumors usually appear in women

  12. Prolapso de tuba uterina após histerectomia vaginal: relato de caso Fallopian tube prolapse after vaginal hysterectomy: a case report

    Directory of Open Access Journals (Sweden)

    Maurício B. Noviello

    2003-12-01

    Full Text Available O prolapso de tuba uterina é complicação rara após histerectomia, com aproximadamente 80 casos descritos na literatura. A sintomatologia é inespecífica, podendo incluir sangramento genital, dispareunia e dor pélvica crônica. O diagnóstico diferencial deve ser feito com granuloma de cúpula vaginal e carcinoma de vagina. O tratamento deve ser individualizado, podendo ser realizado por via vaginal, abdominal ou laparoscópica. Relatamos o caso de uma paciente, 47 anos, com miomatose uterina, submetida a histerectomia vaginal, evoluindo com prolapso de tuba uterina após 11 meses de pós-operatório. O exame especular evidenciava lesão vegetante, friável e sangrante localizada na cúpula vaginal. Esses achados clínicos sugeriam o diagnóstico de prolapso de tuba uterina. A paciente foi submetida a nova intervenção cirúrgica, com ressecção da tuba uterina por via vaginal. O exame natomopatológico confirmou o diagnóstico e a paciente evoluiu com remissão completa da sintomatologia.Fallopian tube prolapse is a rare complication after hysterectomy, with approximately 80 cases described in the literature. The symptoms are nonspecific including vaginal bleeding, dyspareunia and chronic pelvic pain. The differential diagnosis must be done with granulation tissue of the vaginal cuff and vaginal carcinoma. The treatment should be individualized, and is possible to be done by vaginal, abdominal or laparoscopic approach. We report a case of a 47-year-old woman with myoma who was submitted to a vaginal hysterectomy and evolved with fallopian tube prolapse 11 months after surgery. Specular examination showed a fungating, friable and bleeding lesion at the vaginal cuff. The clinical findings suggested the diagnosis of fallopian tube prolapse. The patient was submitted to a new surgical intervention with resection of the left fallopian tube. The histologic examination confirmed the diagnosis and the patient evolved with complete remission of

  13. Aprendizaje cooperativo con alumnos de altas capacidades

    OpenAIRE

    Herrera Lara, Inmaculada

    2016-01-01

    El presente Trabajo Fin de Grado tiene como finalidad conocer e investigar la intervención educativa que se lleva a cabo con los alumnos de altas capacidades a través del aprendizaje cooperativo. Estos alumnos necesitan de la ayuda y colaboración de los otros para lograr verdaderos procesos de aprendizaje. Sin la colaboración de docentes, padres y demás organismos del centro educativo no sería posible ofrecer una respuesta válida y eficaz a las necesidades que presenta este colectivo. Tal com...

  14. Spontaneous actin dynamics in contractile rings

    Science.gov (United States)

    Kruse, Karsten; Wollrab, Viktoria; Thiagarajan, Raghavan; Wald, Anne; Riveline, Daniel

    Networks of polymerizing actin filaments are known to be capable to self-organize into a variety of structures. For example, spontaneous actin polymerization waves have been observed in living cells in a number of circumstances, notably, in crawling neutrophils and slime molds. During later stages of cell division, they can also spontaneously form a contractile ring that will eventually cleave the cell into two daughter cells. We present a framework for describing networks of polymerizing actin filaments, where assembly is regulated by various proteins. It can also include the effects of molecular motors. We show that the molecular processes driven by these proteins can generate various structures that have been observed in contractile rings of fission yeast and mammalian cells. We discuss a possible functional role of each of these patterns. The work was supported by Agence Nationale de la Recherche, France, (ANR-10-LABX-0030-INRT) and by Deutsche Forschungsgemeinschaft through SFB1027.

  15. Capacidad emulsionante de sueros de soja

    Directory of Open Access Journals (Sweden)

    Jorge Wagner

    2011-05-01

    Full Text Available El objetivo fue analizar la capacidad emulsionante de diferentes sueros de soja como productos integrales. Se ensayaron: suero de soja liofilizado (SSL, suero de soja liofilizado y calentado (SSLC, suero de tofu liofilizado (STL y suero de tofu secado térmicamente (STST. Las emulsiones fueron realizadas a igual nivel de proteína bruta (0,1.1,0% p/v, buffer fosfato 10 mm, ƒ³masico =0,33, Ultraturrax T-25. La estabilidad de las emulsiones (medida a traves del aceite separado decrecio en el orden: SSLC > STST > STL > SSL. Estos resultados se correlacionan con el índice de actividad emulsificante con y sin SDS. Por medidas de BackScattering y difracción láser se observo una reducción del tamaño de partícula en las emulsiones preparadas con sueros calentados (SSLC respecto a SSL y STST respecto a STL. Se observaron diferencias en la capacidad emulsionante de SSLC y STST, atribuidas a las condiciones de calentamiento. Las reacciones de desnaturalización y de formación de agregadosglicosilados en el suero de soja liofilizado y calentado reducirían la formación de floculos en la emulsión, principal mecanismode desestabilización en estas emulsiones.

  16. Embolização arterial seletiva em fístula arteriovenosa uterina pós-traumática Selective arterial embolization of uterine post-traumatic arteriovenous fistula

    Directory of Open Access Journals (Sweden)

    Omero Benedicto Poli-Neto

    2004-08-01

    Full Text Available Malformações arteriovenosas uterinas são pouco freqüentes. Os autores relatam um caso de fístula arteriovenosa traumática tratada por embolização seletiva das artérias uterinas, método que tem sido utilizado no controle da hemorragia pós-parto e hemorragia resultante de malignidade pélvica. Uma discussão é apresentada para ressaltar uma conseqüência da perfuração uterina e o controle da hemorragia sem sacrificar a fertilidade.Uterine arteriovenous malformations are uncommon. The authors report a case of traumatic arteriovenous fistula treated by selective uterine arteries embolization. Selective artery embolization has been effectively used to control postpartum hemorrhage and hemorrhage resulting from pelvic malignancy. A discussion on the consequences of uterine perforation and the control of hemorrhage without sacrificing fertility is presented.

  17. Fatores determinantes da capacidade funcional entre idosos

    Directory of Open Access Journals (Sweden)

    Tereza Etsuko da Costa Rosa

    2003-02-01

    Full Text Available OBJETIVO; Investigar a influência de fatores socioeconômicos e demográficos relativos à saúde, bem como os fatores ligados às atividades sociais e à avaliação subjetiva da saúde sobre a capacidade funcional dos idosos. MÉTODOS: Estudo transversal, integrante de estudo multicêntrico, em amostra representativa do município de São Paulo, realizado em 1989. A capacidade funcional foi avaliada através da escala de atividades da vida diária pessoal e instrumental e investigada como variável dicotômica: ausência de dependência - incapacidade/dificuldade em nenhuma das atividades versus presença de dependência moderada/grave - incapacidade/dificuldade em 4 ou mais atividades. Análise de regressão logística múltipla foi aplicada aos fatores hierarquicamente agrupados. RESULTADOS: As características que se associaram com a dependência moderada/grave foram analfabetismo, ser aposentado, ser pensionista, ser dona de casa, não ser proprietário da moradia, ter mais de 65 anos, ter composição familiar multigeracional, ter sido internado nos últimos 6 meses, ser "caso" no rastreamento de saúde mental, não visitar amigos, ter problemas de visão, ter história de derrame, não visitar parentes e ter avaliação pessimista da saúde ao se comparar com seus pares. CONCLUSÕES: As características identificadas que se associaram à dependência moderada/grave sugerem uma complexa rede causal do declínio da capacidade funcional. Pode-se supor, entretanto, que ações preventivas especificamente voltadas para certos fatores podem propiciar benefícios para o prolongamento do bem estar da população idosa.

  18. Cell stiffness, contractile stress and the role of extracellular matrix

    Energy Technology Data Exchange (ETDEWEB)

    An, Steven S., E-mail: san@jhsph.edu [Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street, Room E-7616, Baltimore, MD 21205 (United States); Kim, Jina [Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street, Room E-7616, Baltimore, MD 21205 (United States); Ahn, Kwangmi [Division of Biostatistics, Penn State College of Medicine, Hershey, PA 17033 (United States); Trepat, Xavier [CIBER, Enfermedades Respiratorias, 07110 Bunyola (Spain); Drake, Kenneth J. [Division of Molecular and Integrative Physiological Sciences, Harvard School of Public Health, Boston, MA 02115 (United States); Kumar, Sarvesh; Ling, Guoyu; Purington, Carolyn; Rangasamy, Tirumalai; Kensler, Thomas W.; Mitzner, Wayne [Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street, Room E-7616, Baltimore, MD 21205 (United States); Fredberg, Jeffrey J. [Division of Molecular and Integrative Physiological Sciences, Harvard School of Public Health, Boston, MA 02115 (United States); Biswal, Shyam [Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street, Room E-7616, Baltimore, MD 21205 (United States); Division of Pulmonary and Critical Care Medicine, Johns Hopkins School of Medicine, Baltimore, MD 21205 (United States)

    2009-05-15

    Here we have assessed the effects of extracellular matrix (ECM) composition and rigidity on mechanical properties of the human airway smooth muscle (ASM) cell. Cell stiffness and contractile stress showed appreciable changes from the most relaxed state to the most contracted state: we refer to the maximal range of these changes as the cell contractile scope. The contractile scope was least when the cell was adherent upon collagen V, followed by collagen IV, laminin, and collagen I, and greatest for fibronectin. Regardless of ECM composition, upon adherence to increasingly rigid substrates, the ASM cell positively regulated expression of antioxidant genes in the glutathione pathway and heme oxygenase, and disruption of a redox-sensitive transcription factor, nuclear erythroid 2 p45-related factor (Nrf2), culminated in greater contractile scope. These findings provide biophysical evidence that ECM differentially modulates muscle contractility and, for the first time, demonstrate a link between muscle contractility and Nrf2-directed responses.

  19. Cell stiffness, contractile stress and the role of extracellular matrix

    International Nuclear Information System (INIS)

    Here we have assessed the effects of extracellular matrix (ECM) composition and rigidity on mechanical properties of the human airway smooth muscle (ASM) cell. Cell stiffness and contractile stress showed appreciable changes from the most relaxed state to the most contracted state: we refer to the maximal range of these changes as the cell contractile scope. The contractile scope was least when the cell was adherent upon collagen V, followed by collagen IV, laminin, and collagen I, and greatest for fibronectin. Regardless of ECM composition, upon adherence to increasingly rigid substrates, the ASM cell positively regulated expression of antioxidant genes in the glutathione pathway and heme oxygenase, and disruption of a redox-sensitive transcription factor, nuclear erythroid 2 p45-related factor (Nrf2), culminated in greater contractile scope. These findings provide biophysical evidence that ECM differentially modulates muscle contractility and, for the first time, demonstrate a link between muscle contractility and Nrf2-directed responses.

  20. Modelo computacional para caracterización de células escamosas de citologías cérvico-uterinas

    OpenAIRE

    Martínez Abaunza Víctor Eduardo; Mendoza Castellanos Alfonso; Uribe Pérez Claudia Janeth

    2007-01-01

    El trabajo se realizó entre el Grupo de Investigación en Ingeniería Biomédica (GIIB) y el Grupo de Investigación en Patología Estructural, Funcional y Clínica de la Universidad Industrial de Santander (UIS), junto con la Facultad de Medicina de la Universidad Autónoma de Bucaramanga (UNAB); el objetivo principal es construir un modelo computacional que permita caracterizar las células presentes en una citología cérvico uterina, con el propósito de clasificarlas como normales o displásicas. La...

  1. Embolização uterina para tratamento de mioma sintomático: experiência inicial revisão da literatura

    Directory of Open Access Journals (Sweden)

    Kisilevzky Nestor Hugo

    2003-01-01

    Full Text Available OBJETIVO: Apresentar os resultados da experiência clínica inicial de 100 casos de mulheres portadoras de miomatose sintomática que foram submetidas a embolização das artérias uterinas como forma de tratamento principal. Apresenta-se, também, extensa revisão bibliográfica sobre o tema, para determinar as indicações e contra-indicações, bem como as eventuais complicações do método. MATERIAL E MÉTODO: Cem pacientes com miomatose sintomática foram submetidas a embolização das artérias uterinas como única forma de tratamento. O principal sintoma que indicou a intervenção foi o aumento do fluxo menstrual em 79 pacientes e dor associada à miomatose em 21. O diagnóstico de miomatose foi realizado por meio de ultra-sonografia em 75 pacientes, e pela associação de ultra-sonografia e ressonância magnética em 25 pacientes. O volume uterino médio avaliado por esses métodos de imagem resultou em 487 cmsuperscript three. Os procedimentos foram realizados em duas instituições hospitalares: uma pública, onde foram atendidas 56 pacientes dependentes do Sistema Único da Saúde (SUS, e outra particular, onde foram atendidas 46 pacientes com plano de assistência médica. Setenta e seis pacientes foram avaliadas clinicamente após 12 semanas da realização da embolização uterina. RESULTADOS: O procedimento foi completado com sucesso em 97% dos casos, utilizando-se técnica convencional. O acompanhamento e a avaliação clínica após 12 semanas evidenciou que houve melhora dos sintomas em mais de 90% das pacientes. Verificou-se, ainda, redução de volume uterino de 52%. Não foram observadas complicações técnicas ou clínicas relevantes. CONCLUSÃO: A técnica de embolização uterina para tratamento da miomatose sintomática é um método simples, eficiente e seguro.

  2. Malformação arteriovenosa uterina após doença trofoblástica gestacional Uterine arteriovenous malformation after gestational trophoblastic disease

    OpenAIRE

    Paulo Belfort; Antônio Braga; Nazaré Serra Freire

    2006-01-01

    OBJETIVO: investigar a presença e resultados de malformações vasculares uterinas (MAVU) após doença trofoblástica gestacional (DTG). MÉTODOS: estudo retrospectivo com inclusão de casos diagnosticados entre 1987 e 2004; 2764 pacientes após DTG foram acompanhadas anualmente com ultra-sonografia transvaginal e Doppler colorido no Centro de Neoplasia Trofoblástica Gestacional da Santa Casa da Misericórdia (Rio de Janeiro, RJ, Brasil). Sete pacientes tiveram diagnóstico final de MAVU baseado em an...

  3. Tamizaje y prevención de pre-eclampsia guiado por Doppler de arterias uterinas: revisión sistemática de la literatura

    OpenAIRE

    Nicolás Sáez O; Jorge Carvajal C

    2012-01-01

    Antecedentes: La pre-eclampsia (PE) es una enfermedad grave asociada al embarazo con una prevalencia de ~3%. La identificación de las mujeres en riesgo podría mejorar el resultado materno y perinatal. La estimación de riesgo individual de PE permitiría ofrecer un tratamiento preventivo para ésta [ej: aspirina (AAS)]. Se ha descrito el Doppler de arterias uterinas (DAUt) en primer y segundo trimestre como un método de tamizaje de PE. Objetivo: Recopilar la evidencia disponible sobre el uso del...

  4. Molecular Model of the Contractile Ring

    CERN Document Server

    Biron, D; Tlusty, Tsvi; Moses, Elisha; 10.1103/PhysRevLett.95.098102

    2010-01-01

    We present a model for the actin contractile ring of adherent animal cells. The model suggests that the actin concentration within the ring and consequently the power that the ring exerts both increase during contraction. We demonstrate the crucial role of actin polymerization and depolymerization throughout cytokinesis, and the dominance of viscous dissipation in the dynamics. The physical origin of two phases in cytokinesis dynamics ("biphasic cytokinesis") follows from a limitation on the actin density. The model is consistent with a wide range of measurements of the midzone of dividing animal cells.

  5. A altura uterina é capaz de diagnosticar os desvios do volume de líquido amniótico? Is uterine height able to diagnose amniotic fluid volume deviations?

    Directory of Open Access Journals (Sweden)

    Djacyr Magna Cabral Freire

    2013-02-01

    Full Text Available OBJETIVO: Avaliar o desempenho de uma curva de altura uterina (AU quanto à capacidade de rastrear desvios do volume de líquido amniótico, utilizando uma curva brasileira de índice de líquido amniótico (ILA como padrão-ouro. MÉTODOS: O presente estudo representa um corte transversal no qual foram incluídas 753 gestantes em acompanhamento pré-natal na rede pública de João Pessoa (PB no período de março a outubro de 2006 e que tiveram um exame de ultrassonografia (US de rotina agendado para depois da 26ª semana de idade gestacional. Foram excluídos os casos com diagnóstico de gestação gemelar, óbito fetal intrauterino e malformações fetais maiores. Além de informações sociodemográficas, foram coletados também os valores da AU medida de forma padronizada, os valores do peso fetal estimado, do ILA e a idade gestacional pelo exame de US. A capacidade da curva de AU em predizer os desvios do volume de líquido amniótico foi avaliada tendo uma curva brasileira de ILA em função da idade gestacional como padrão-ouro. Para isso, foram estimados a sensibilidade, especificidade e valores preditivos positivo e negativo para diferentes pontos de corte. RESULTADOS: A medida da AU identificou 10,5% das mulheres como AU baixa e possivelmente associada ao oligoâmnio, e 25,2% como AU alta e possivelmente associada ao polidrâmnio. Utilizando uma curva brasileira de referência para ILA, a AU foi capaz de predizer pobremente a ocorrência de oligoâmnio (sensibilidade variando entre 37 a 28% e de forma razoável a ocorrência de polidrâmnio (sensibilidade variando entre 88 a 69%. CONCLUSÃO: A medida da altura uterina mostrou um desempenho ruim para predizer oligoâmnio e um desempenho razoável para predizer polidrâmnio. Sua utilização para essa finalidade só se justifica, portanto, em situações nas quais o exame ultrassonográfico não esteja fácil e rotineiramente disponível, a fim de ajudar na priorização dos casos que

  6. Contractility Dispersion in Long QT Syndrome

    Directory of Open Access Journals (Sweden)

    MH Nikoo

    2009-09-01

    Full Text Available Background: Previous studies, using M mode echocardiography, provided unexpected evidence of a mechanical alteration in patients with long QT syndrome. The aim of this study was to evaluate entire left ventricular (LV wall motion characteristics in patients with long QT syndrome using tissue Doppler imaging. Methods: We enrolled 17 patients with congenital long QT syndrome [11 female and 6 male], aged 21 to 45 years. 10 subjects without cardiac disease were also selected as a control group. Two-dimensional tissue Doppler imaging (TDI recording of the LV was obtained from the basal and mid-segments from apical four-chamber, two-chamber, and long-axis views. ‘Myocardial Contraction Duration’ [MCD] was defined as the time from start of R wave on ECG to end of S wave on TDI. MCD was measured in the six LV wall positions: septal, anteroseptal, lateral, inferior, posterior and anterior positions.Results: LV contractility dispersion was significantly greater in long QT syndrome patients compared to control group [0.051 ± 0.011 vs. 0.016 ± 0.06; P < 0.001]. Conclusion: Our study evaluated left ventricular dispersion of contractility duration in patients with long QT syndrome. This mechanical dispersion may be a reflection of the inhomogeneity of repolarisation in the long QT syndrome.

  7. A quantitative analysis of contractility in active cytoskeletal protein networks.

    Science.gov (United States)

    Bendix, Poul M; Koenderink, Gijsje H; Cuvelier, Damien; Dogic, Zvonimir; Koeleman, Bernard N; Brieher, William M; Field, Christine M; Mahadevan, L; Weitz, David A

    2008-04-15

    Cells actively produce contractile forces for a variety of processes including cytokinesis and motility. Contractility is known to rely on myosin II motors which convert chemical energy from ATP hydrolysis into forces on actin filaments. However, the basic physical principles of cell contractility remain poorly understood. We reconstitute contractility in a simplified model system of purified F-actin, muscle myosin II motors, and alpha-actinin cross-linkers. We show that contractility occurs above a threshold motor concentration and within a window of cross-linker concentrations. We also quantify the pore size of the bundled networks and find contractility to occur at a critical distance between the bundles. We propose a simple mechanism of contraction based on myosin filaments pulling neighboring bundles together into an aggregated structure. Observations of this reconstituted system in both bulk and low-dimensional geometries show that the contracting gels pull on and deform their surface with a contractile force of approximately 1 microN, or approximately 100 pN per F-actin bundle. Cytoplasmic extracts contracting in identical environments show a similar behavior and dependence on myosin as the reconstituted system. Our results suggest that cellular contractility can be sensitively regulated by tuning the (local) activity of molecular motors and the cross-linker density and binding affinity. PMID:18192374

  8. Genome sequence of Haloplasma contractile, an unusual contractile bacterium from a deep-sea anoxic brine lake.

    KAUST Repository

    Antunes, Andre

    2011-09-01

    We present the draft genome of Haloplasma contractile, isolated from a deep-sea brine and representing a new order between Firmicutes and Mollicutes. Its complex morphology with contractile protrusions might be strongly influenced by the presence of seven MreB/Mbl homologs, which appears to be the highest copy number ever reported.

  9. Considerations for Contractile Electroactive Materials and Actuators

    Energy Technology Data Exchange (ETDEWEB)

    Lenore Rasmussen, David Schramm, Paul Rasmussen, Kevin Mullaly, Ras Labs, LLC, Intelligent Materials for Prosthetics & Automation, Lewis D. Meixler, Daniel Pearlman and Alice Kirk

    2011-05-23

    Ras Labs produces contractile electroactive polymer (EAP) based materials and actuators that bend, swell, ripple, and contract (new development) with low electric input. In addition, Ras Labs produces EAP materials that quickly contract and expand, repeatedly, by reversing the polarity of the electric input, which can be cycled. This phenomenon was explored using molecular modeling, followed by experimentation. Applied voltage step functions were also investigated. High voltage steps followed by low voltage steps produced a larger contraction followed by a smaller contraction. Actuator control by simply adjusting the electric input is extremely useful for biomimetic applications. Muscles are able to partially contract. If muscles could only completely contract, nobody could hold an egg, for example, without breaking it. A combination of high and low voltage step functions could produce gross motor function and fine manipulation within the same actuator unit. Plasma treated electrodes with various geometries were investigated as a means of providing for more durable actuation.

  10. La capacidad desfaunante del extracto de plantas en el rumen

    OpenAIRE

    Alejandro Ley de Coss; Jaime Jorge Martínez Tinajero; Francisco Javier Marroquín Agreda; Carlos Gumaro García Castillo; Oziel Dante Montañez Valdez; Enrique Guerra Medina

    2011-01-01

    El objetivo del presente estudio fue evaluar la capacidad de eliminar protozoarios del rumen con el uso del extracto soluble en agua de las plantas Buddleia cordata, Chenopodium ambrosioides, Datura inoxia, Marrubium vulgare, Mentha piperita y Verbesina perymenioides, las cuales contienen compuestos secundarios con posible efecto tóxico sobre los protozoarios ciliados del rumen. La capacidad desfaunante (CD) del extracto soluble en agua de las plantas se realizó inoculando 0,5 mL de un concen...

  11. EL CAPITAL ESTRUCTURAL Y LA CAPACIDAD INNOVADORA DE LA EMPRESA

    Directory of Open Access Journals (Sweden)

    Santos-Rodrigues, Helena

    2011-09-01

    Full Text Available El entorno empresarial actual presiona a las empresas y a los investigadores para que busquen nuevos factores que permitan alcanzar y mantener sus ventajas competitivas. Entre estos se encuentra cada vez con más importancia el capital intelectual. Un componente representativo de los activos estratégicos de conocimiento, de la propiedad de la empresa, entre otros aspectos, es el capital estructural. Es habitual sospechar que éste afecta a la capacidad innovadora. Sin embargo, es menos conocida la forma en que se produce ese impacto y los aspectos que intervienen. Para investigar esta conexión realizamos una encuesta a 68 empresas que trabajan en el sector de componentes para automoción localizadas en Galicia (Norte de España y el Norte de Portugal. Los resultados muestran, por un lado, que la capacidad innovadora tiene dos dimensiones: la capacidad innovadora de producto - proceso y la capacidad innovadora de gestión; por otro lado, que el capital estructural influye de forma diferenciada en cada tipo de capacidad innovadora. Verificamos que influye directamente en la capacidad innovadora de gestión.

  12. Estudio macro-microscópico de la involución uterina postparto en Alpacas (Macro and microscopic of uterine involution postpartum in alpacas summary)

    OpenAIRE

    MVZ. Augusto Excelmes Loza.

    2006-01-01

    El presente estudio se llevo a cabo en el Laboratorio de Histología y Embriología de la Facultad de Medicina Veterinaria y Zootecnia de la Universidad Nacional del Altiplano; con alpacas provenientes de la Rural Alianza E.P.S. (4,140m.s.n.m), Centro Experimental Quinsachata-INIA (4,050 m.s.n.m) y el Centro de Investigación y Producción La Raya (4,130 m.s.n.m), cuyo objetivo fue el estudio del proceso de la involución uterina post parto en la alpaca, desde el parto hasta los 30dpp. Para tal pr...

  13. Estudio macro-microscópico de la involución uterina postparto en alpacas - Macro and microscopic of uterine involution postpartum in alpacas

    OpenAIRE

    MVZ. Augusto Excelmes Loza. Puno-Perú 2005

    2006-01-01

    El presente estudio se llevo a cabo en el Laboratorio de Histología y Embriología de la Facultad de Medicina Veterinaria y Zootecnia de la Universidad Nacional del Altiplano; con alpacas provenientes de la Rural Alianza E.P.S. (4,140m.s.n.m), Centro Experimental Quinsachata-INIA (4,050 m.s.n.m) y el Centro de Investigación y Producción La Raya (4,130 m.s.n.m), cuyo objetivo fue el estudio del proceso de la involución uterina post parto en la alpaca, desde el parto hasta los 30dpp. Para tal pr...

  14. Nova pipeta para inseminação intra-uterina em suínos New pipette for intrauterine insemination in pigs

    OpenAIRE

    Gustavo Nogueira Diehl; Wald'ma Sobrinho Amaral Filha; Rafael Kummer; Felipe Koller; Mari Lourdes Bernardi; Ivo Wentz; Fernando Pandolfo Bortolozzo

    2006-01-01

    O objetivo deste trabalho foi avaliar o desempenho reprodutivo de 423 fêmeas suínas de ordem de parto 1 a 9 submetidas à inseminação intra-uterina (IAU), com um novo modelo de pipeta (T1) cuja extremidade não é fixada na cérvix ou uma pipeta de IAU modelo Verona® e que permite a fixação da sua extremidade em espiral na cérvix (T2). Para comparar as duas pipetas, foi considerado o grau de dificuldade para realização das inseminações, o tempo necessário para realizá-las, a presença de sangramen...

  15. Considerations for Contractile Electroactive Materials and Actuators

    Energy Technology Data Exchange (ETDEWEB)

    Rasmussen, Lenore; Erickson, Carl J.; Meixler, Lewis D.; Ascione, George; Gentile, Charles A.; Tilson, Carl; Bernasek, Stephen L.; Abelev, Esta

    2010-02-19

    Ras Labs produces electroactive polymer (EAP) based materials and actuators that bend, swell, ripple and now contract (new development) with low electric input. This is an important attribute because of the ability of contraction to produce life-like motion. The mechanism of contraction is not well understood. Radionuclide-labeled experiments were conducted to follow the movement of electrolytes and water in these EAPs when activated. Extreme temperature experiments were performed on the contractile EAPs with very favorable results. One of the biggest challenges in developing these actuators, however, is the electrode-EAP interface because of the pronounced movement of the EAP. Plasma treatments of metallic electrodes were investigated in order to improve the attachment of the embedded electrodes to the EAP material. Surface analysis, adhesive testing, and mechanical testing were conducted to test metal surfaces and metal-polymer interfaces. The nitrogen plasma treatment of titanium produced a strong metal-polymer interface; however, oxygen plasma treatment of both stainless steel and titanium produced even stronger metal-polymer interfaces. Plasma treatment of the electrodes allows for the embedded electrodes and the EAP material of the actuator to work and move as a unit, with no detachment, by significantly improving the metal-polymer interface.

  16. Enriquecimiento curricular para el alumnado con altas capacidades en primaria

    OpenAIRE

    Boldú Caballero, Tomás

    2013-01-01

    La finalidad de este documento es proporcionar al lector, primero, una rápida ojeada a la trayectoria histórica de la atención a las NEE (más concretamente de las altas capacidades); segundo, una conceptualización de las altas capacidades y sus necesidades educativas específicas tomando como punto de referencia algunos de lo más eminentes autores y corrientes psicopedagógicas actuales o del pasado reciente; y por último, facilitar ejemplos prácticos desde la perspectiva del doc...

  17. Capacidad funcional y salud: orientaciones para cuidar al adulto mayor

    OpenAIRE

    CLARA INÉS GIRALDO M; GLORIA MARÍA FRANCO A

    2008-01-01

    Objetivo: valorar la salud y la capacidad funcional de ancianos con dependencia funcional para el autocuidado, como elementos para orientar el cuidado de enfermería y el cuidado familiar en casa. Metodología: estudio descriptivo transversal en 40 personas de 65 y más años con dependencia funcional, cuidados por familiar, en Envigado Colombia; muestreo por conveniencia. Valoración de la capacidad funcional con el índice de Katz y la escala de Lawton y Brody, ajustados según grupo de Neurocienc...

  18. Capital humano y capacidad humana Human capital and human capacity

    OpenAIRE

    Sen Amartya Kumar

    1998-01-01

    En este articulo se examinan las relaciones y diferencias entre el concepto de 'capital humano' y el concepto de 'capacidad humana'. El concepto de capital humano es mas limitado puesto que solo concibe las cualidades humanas en su relación con el crecimiento económico mientras que el concepto de capacidades da énfasis a la expansión de libertad humana para vivir el tipo de vida que la gente considera valedera. Cuando se adopta esa visión mas amplia, el proceso de desarrollo no puede verse si...

  19. Estudo da involução uterina por meio da ultra-sonografia (modo-B) em cadelas submetidas a cesariana Ultrasonographyc study of the postpartum uterine involution in bitches after cesaerean section

    OpenAIRE

    S.T.S. Ferri; W.R.R. Vicente; G.H. Toniollo

    2003-01-01

    Utilizaram-se 15 cadelas gestantes submetidas a cesariana e posteriormente a exames ultra-sonográficos seriados, em modo B (tempo real), para averiguação do diâmetro uterino nos dias 0, 3, 7, 14 e 21 pós-cesariana, com o objetivo de verificar a influência do ato cirúrgico na involução uterina. Os resultados (média e desvio-padrão, em centímetros) registrados para os dias estudados foram, respectivamente: 3,99+0,71; 3,27+0,51; 2,60+0,54; 2,01+0,34; 1,28+0,24. A involução uterina pós-cesariana ...

  20. Dopplervelocimetria das artérias uterinas na predição de complicações em gestações de baixo risco Uterine artery doppler velocimetry for the prediction of complications in low-risk pregnancies

    OpenAIRE

    Fabrício da Silva Costa; Sérgio Pereira da Cunha; Aderson Tadeu Berezowsky

    2005-01-01

    OBJETIVO: determinar a validade da dopplervelocimetria das artérias uterinas na predição de complicações da gestação em população de nulíparas de baixo risco. MÉTODOS: foi conduzido estudo prospectivo que incluiu 45 pacientes primigestas, sem história de doenças crônicas. A dopplervelocimetria das artérias uterinas foi realizada entre 24 e 26 semanas, com determinação do índice de resistência (IR), índice de pulsatilidade (IP), relação S/D e a presença ou ausência de incisura na onda de veloc...

  1. Role of microtubules in the contractile dysfunction of hypertrophied myocardium

    Science.gov (United States)

    Zile, M. R.; Koide, M.; Sato, H.; Ishiguro, Y.; Conrad, C. H.; Buckley, J. M.; Morgan, J. P.; Cooper, G. 4th

    1999-01-01

    OBJECTIVES: We sought to determine whether the ameliorative effects of microtubule depolymerization on cellular contractile dysfunction in pressure overload cardiac hypertrophy apply at the tissue level. BACKGROUND: A selective and persistent increase in microtubule density causes decreased contractile function of cardiocytes from cats with hypertrophy produced by chronic right ventricular (RV) pressure overloading. Microtubule depolymerization by colchicine normalizes contractility in these isolated cardiocytes. However, whether these changes in cellular function might contribute to changes in function at the more highly integrated and complex cardiac tissue level was unknown. METHODS: Accordingly, RV papillary muscles were isolated from 25 cats with RV pressure overload hypertrophy induced by pulmonary artery banding (PAB) for 4 weeks and 25 control cats. Contractile state was measured using physiologically sequenced contractions before and 90 min after treatment with 10(-5) mol/liter colchicine. RESULTS: The PAB significantly increased RV systolic pressure and the RV weight/body weight ratio in PAB; it significantly decreased developed tension from 59+/-3 mN/mm2 in control to 25+/-4 mN/mm2 in PAB, shortening extent from 0.21+/-0.01 muscle lengths (ML) in control to 0.12+/-0.01 ML in PAB, and shortening rate from 1.12+/-0.07 ML/s in control to 0.55+/-0.03 ML/s in PAB. Indirect immunofluorescence confocal microscopy showed that PAB muscles had a selective increase in microtubule density and that colchicine caused complete microtubule depolymerization in both control and PAB papillary muscles. Microtubule depolymerization normalized myocardial contractility in papillary muscles of PAB cats but did not alter contractility in control muscles. CONCLUSIONS: Excess microtubule density, therefore, is equally important to both cellular and to myocardial contractile dysfunction caused by chronic, severe pressure-overload cardiac hypertrophy.

  2. Dietary Nitrate and Skeletal Muscle Contractile Function in Heart Failure.

    Science.gov (United States)

    Coggan, Andrew R; Peterson, Linda R

    2016-08-01

    Heart failure (HF) patients suffer from exercise intolerance that diminishes their ability to perform normal activities of daily living and hence compromises their quality of life. This is due largely to detrimental changes in skeletal muscle mass, structure, metabolism, and function. This includes an impairment of muscle contractile performance, i.e., a decline in the maximal force, speed, and power of muscle shortening. Although numerous mechanisms underlie this reduction in contractility, one contributing factor may be a decrease in nitric oxide (NO) bioavailability. Consistent with this, recent data demonstrate that acute ingestion of NO3 (-)-rich beetroot juice, a source of NO via the NO synthase-independent enterosalivary pathway, markedly increases maximal muscle speed and power in HF patients. This review discusses the role of muscle contractile dysfunction in the exercise intolerance characteristic of HF, and the evidence that dietary NO3 (-) supplementation may represent a novel and simple therapy for this currently underappreciated problem. PMID:27271563

  3. Geometrical origins of contractility in disordered actomyosin networks

    CERN Document Server

    Lenz, Martin

    2014-01-01

    Movement within eukaryotic cells largely originates from localized forces exerted by myosin motors on scaffolds of actin filaments. Although individual motors locally exert both contractile and extensile forces, large actomyosin structures at the cellular scale are overwhelmingly contractile, suggesting that the scaffold serves to favor contraction over extension. While this mechanism is well understood in highly organized striated muscle, its origin in disordered networks such as the cell cortex is unknown. Here we develop a mathematical model of the actin scaffold's local two- or three-dimensional mechanics and identify four competing contraction mechanisms. We predict that one mechanism dominates, whereby local deformations of the actin break the balance between contraction and extension. In this mechanism, contractile forces result mostly from motors plucking the filaments transversely rather than buckling them longitudinally. These findings sheds light on recent $\\textit{in vitro}$ experiments, and provi...

  4. Spontaneous oscillations from turnover of an elastic contractile material

    CERN Document Server

    Dierkes, Kai; Solon, Jérôme; Salbreux, Guillaume

    2014-01-01

    Single and collective cellular oscillations involving the actomyosin cytoskeleton have been observed in numerous biological systems. We show here that a generic model of a contractile material, which is turning over and contracts against an elastic element, exhibits spontaneous oscillations. Such a model can thus account for shape oscillations observed in amnioserosa cells during dorsal closure of the Drosophila embryo. We investigate the collective dynamics of an ensemble of such oscillators and show that the relative contribution of viscous and friction losses yield different regimes of collective oscillations. Taking into account the diffusion of contractile elements, our theoretical framework predicts the appearance of traveling waves which might account for the propagation of actomyosin contractile waves observed during morphogenesis.

  5. Cuerpos, capacidades, exigencias funcionales... y otros lechos de Procusto Cuerpos, capacidades, exigencias funcionales... y otros lechos de Procusto

    Directory of Open Access Journals (Sweden)

    Mario Toboso Martín

    2010-03-01

    Full Text Available El denominado modelo social de la discapacidad interpreta ésta como una construcción social, pero no presenta de la misma manera la capacidad. Habitualmente se supone que las capacidades lo son propiamente del cuerpo, mientras que se pasa por alto que llegar a poseer un determinado conjunto de capacidades es un hecho normativamente impuesto por nuestros patrones culturales de vida. Las capacidades que definen este conjunto retratan fielmente las capacidades de lo que aquí denominamos cuerpo normativo. La reflexión en torno al cuerpo, como elemento sociocultural clave, debe cuestionar el esencialismo en la atribución de tales capacidades, notando que el primer paso para caer bajo las miradas médica y capacitista es, precisamente, definir y asumir un conjunto previo de capacidades inherentes atribuibles al cuerpo. Como vía para la deconstrucción del cuerpo normativo nos planteamos poner en práctica una mirada alternativa, que se sitúe lejos de la creencia generalizada en un modo de funcionamiento mayoritario y único, y permita también tener en cuenta los modos minoritarios y menos habituales. La reflexión se convierte así en una cuestión de respeto a la diversidad funcional que, como condición inherente al cuerpo y al ser humano, resulta del reconocimiento de todas las expresiones diferentes de funcionamiento posibles.The so-called social model of disability interprets it as a social construction, but it does not present capability in the same manner. Usually abilities are supposed to be inherent to the body, whereas is overlooked that being able to reach certain set of abilities is an imposed normative fact demanded by our cultural life standards. This set defining abilities reflects accurately the abilities of what we call normative body. The reflection around the body, as a key sociocultural element, must question the essentialism in the attribution of such abilities, remarking that the first step to fall under medical and able

  6. Modelo computacional para caracterización de células escamosas de citologías cérvico-uterinas

    Directory of Open Access Journals (Sweden)

    Martínez Abaunza Víctor Eduardo

    2005-12-01

    Full Text Available El trabajo se realizó entre el Grupo de Investigación en Ingeniería Biomédica (GIIB y el Grupo de Investigación en Patología Estructural, Funcional y Clínica de la Universidad Industrial de Santander (UIS, junto con la Facultad de Medicina de la Universidad Autónoma de Bucaramanga (UNAB; el objetivo principal es construir un modelo computacional que permita caracterizar las células presentes en una citología cérvico uterina, con el propósito de clasificarlas como normales o displásicas. Las láminas que contenían las muestras celulares las recolectaron los patólogos y se tomaron fotografías digitales por medio de una cámara de video acoplada a un microscopio y conectada a un dispositivo de adquisición. En la segmentación de las imágenes se utilizaron tres algoritmos de binarización que permitieron detectar los núcleos celulares; debido a que estos algoritmos presentaron fallas en la detección del citoplasma, se utilizó binarización manual. La descripción de la textura celular se logró con la distribución del histograma, en cada uno de los planos de color, y en los bordes se emplearon descriptores de Fourier. Se presentan los resultados de la primera fase, implementados en la clasificación e identificación de células normales. En las fases posteriores se aplicará la caracterización de cada uno de los estadios de las células clasificadas inicialmente como displásicas, para lograr discriminar las reactivas de las que realmente tienen cambios de lesión. Palabras clave: tratamiento de imágenes, morfología matemática, citología cérvico uterina, displasia, cáncer de cérvix.

  7. DIAGNOSIS AND THERAPEUTIC CONSIDERATIONS OF UTERINE INFECTIONS IN DAIRY CATTLE CONSIDERACIONES DIAGNÓSTICAS Y TERAPÉUTICAS PARA INFECCIONES UTERINAS EN VACAS LECHERAS

    Directory of Open Access Journals (Sweden)

    Risco Carlos

    2009-05-01

    Full Text Available From an animal health-well being, welfare, and performance perspective, the postpartum period is comprised of an early window where acute health and cow survival is an issue with the development of septic metritis. Subsequently, sustained presence of endometritis is likely to be contributing to sub-fertility in lactating dairy cows with an overall herd pregnancy rate of approximately 16%. The challenge is to integrate on commercial dairies preventive medicine programs with reproductive management to improve herd fertility. Cows affected by periparturient disorders such as hypocalcemia, dystocia, and retained fetal membranes are more likely to contract uterine infections than cows that calve normally are. Thus, appropriate management of the transition period is critical in the prevention of uterine infections at the herd level. In addition, a postpartum program to monitor health to provide treatment to cows in the early stages of disease should be implemented.Desde el punto de vista del bienestar animal y de la perspectiva de productividad, el periodo postparto puede estar comprometido por una pequeña ventana durante el postparto temprano, en la cual la salud y supervivencia de las vacas es un problemática, debido a la presentación de metritis séptica. Esta se perpetuán subsecuentemente con la presentación de endometritis, la cual muy posiblemente contribuye con los patrones de sub-fertilidad en vacas lactantes, generando en general bajas tasas de preñez del 16 %. El reto es entonces integrar en las explotaciones comerciales un programa de medicina veterinaria preventiva con el manejo reproductivo, con el fin de mejorar la fertilidad del hato. Se ha descrito que las vacas que sufren problemas durante el periparto tales como hipocalcemia y retención de las membranas fetales son más susceptibles de contraer infecciones uterinas, comparadas con aquellas vacas que tienen un parto normal. Por ello un manejo apropiado del periodo de transici

  8. Função endotelial, perfusão uterina e fluxo central em gestações complicadas por Pré-Eclampsia

    Directory of Open Access Journals (Sweden)

    Augusto Henriques Fulgêncio Brandão

    2012-10-01

    Full Text Available FUNDAMENTO: A fisiopatologia da Pré-Eclampsia (PE é caracterizada por deficiência no processo de placentação, disfunção endotelial sistêmica e hiperfluxo do Sistema Nervoso Central (SNC. Do ponto de vista clínico, seria interessante determinar a ocorrência desses fenômenos antes do aparecimento das manifestações clínicas da doença, levantando a possibilidade de novos métodos de predição da PE. OBJETIVO: Comparar o processo de placentação, a função endotelial e o hiperfluxo do SNC em gestantes de alto risco para desenvolvimento de PE que posteriormente desenvolveram ou não a síndrome. MÉTODOS: Um total de 74 gestantes foi submetido ao exame de Dilatação Fluxo-Mediada (DFM da artéria braquial, dopplerfluxometria de artérias uterinas e oftálmica para avaliação da função endotelial, processo de placentação e hiperfluxo central, respectivamente. Os exames foram realizados entre 24 e 28 semanas de gestação e as pacientes foram acompanhadas até o puerpério para coleta de dados. RESULTADOS: Quinze pacientes tiveram a gestação complicada pela PE e 59 se mantiveram normotensas até o puerpério. Pacientes que subsequentemente desenvolveram PE apresentaram entre 24 e 28 semanas de gestação, maiores valores no índice de pulsatilidade das artérias uterinas e menores valores de DFM (p < 0,001 e p = 0,001, respectivamente. Entretanto, não houve diferença nos valores obtidos no índice de resistência da artéria oftálmica (p = 0,08. CONCLUSÃO: Os dados obtidos sugerem que a deficiência no processo de placentação e a disfunção endotelial precedem cronologicamente as manifestações clínicas da PE, o que não ocorre com o hiperfluxo do SNC.

  9. Capacidades intangibles para la competitividad microempresarial en México

    Directory of Open Access Journals (Sweden)

    Noé Fuentes

    2016-01-01

    Full Text Available La competitividad empresarial ha sido objeto de estudio y debate dentro de la literatura económica, estableciendo diferentes factores determinantes para el desarrollo empresarial. En este trabajo, desde el enfoque de la Economía Industrial, la Nueva Economía Industrial y la Teoría de Recursos y Capacidades, se elabora un modelo econométrico de panel con 2 671 microempresas mexicanas a lo largo de cuatro periodos, que detalla la relación de ventajas competitivas de la microempresa con factores externos e internos como la estructura sectorial y los activos tangibles e intangibles de la unidad económica. Los principales resultados encontrados sugieren que la generación de las sinergias adecuadas para el desenvolvimiento del sector productivo de pequeña escala se logra principalmente a partir de las capacidades intangibles.

  10. Metabolism: flow and contractility of the Langendorff heart

    NARCIS (Netherlands)

    H. Stam (Hans)

    1978-01-01

    textabstractThis thesis reviews current literature and describes experimental studies on the regulation and modification of coronary flow and contractility in isolated rat hearts. In chapter I and introduction is given to the problems of fatty acid toxicity and myocardial function. Coronary flow rat

  11. Carbon monoxide affects electrical and contractile activity of rat myocardium

    Directory of Open Access Journals (Sweden)

    Porokhnya Maria V

    2011-06-01

    Full Text Available Abstract Background Carbon monoxide (CO is a toxic gas, which also acts in the organism as a neurotransmitter. It is generated as a by-product of heme breakdown catalyzed by heme oxygenase. We have investigated changes in electrical and contractile activity of isolated rat atrial and ventricular myocardium preparations under the influence of CO. Methods Standard microelectrode technique was used for intracellular registration of electrical activity in isolated preparations of atrial and ventricular myocardium. Contractions of atrial myocardial stripes were registered via force transducer. Results CO (10-4 - 10-3 M caused prominent decrease of action potential duration (APD in working atrial myocardium as well as significant acceleration of sinus rhythm. In addition CO reduced force of contractions and other parameters of contractile activity. Inhibitor of heme oxygenase zinc protoporphyrin IX exerts opposite effects: prolongation of action potential, reduction of sinus rhythm rate and enhancement of contractile function. Therefore, endogenous CO, which may be generated in the heart due to the presence of active heme oxygenase, is likely to exert the same effects as exogenous CO applied to the perfusing medium. In ventricular myocardium preparations exogenous CO also induced shortening of action potential, while zinc protoporphyrin IX produced the opposite effect. Conclusions Thus, endogenous or exogenous carbon monoxide may act as an important regulator of electrical and contractile cardiac activity.

  12. Genetic fuzzy system predicting contractile reactivity patterns of small arteries

    DEFF Research Database (Denmark)

    Tang, J; Sheykhzade, Majid; Clausen, B F;

    2014-01-01

    strategies. Results show that optimized fuzzy systems (OFSs) predict contractile reactivity of arteries accurately. In addition, OFSs identified significant differences that were undetectable using conventional analysis in the responses of arteries between groups. We concluded that OFSs may be used in...

  13. Myocardial contractile function in survived neonatal piglets after cardiopulmonary bypass

    Directory of Open Access Journals (Sweden)

    Popov Aron-Frederik

    2010-11-01

    Full Text Available Abstract Background Hemodynamic function may be depressed in the early postoperative stages after cardiac surgery. The aim of this study was the analysis of the myocardial contractility in neonates after cardiopulmonary bypass (CPB and mild hypothermia. Methods Three indices of left ventricular myocardial contractile function (dP/dt, (dP/dt/P, and wall thickening were studied up to 6 hours after CPB in neonatal piglets (CPB group; n = 4. The contractility data were analysed and then compared to the data of newborn piglets who also underwent median thoracotomy and instrumentation for the same time intervals but without CPB (non-CPB group; n = 3. Results Left ventricular dP/dtmax and (dP/dtmax/P remained stable in CPB group, while dP/dtmax decreased in non-CPB group 5 hours postoperatively (1761 ± 205 mmHg/s at baseline vs. 1170 ± 205 mmHg/s after 5 h; p max and (dP/dtmax/P there were no statistically significant differences between the two groups. Comparably, although myocardial thickening decreased in the non-CPB group the differences between the two groups were not statistically significant. Conclusions The myocardial contractile function in survived neonatal piglets remained stable 6 hours after cardiopulmonary bypass and mild hypothermia probably due to regional hypercontractility.

  14. Cuaderno de Prácticas: Altas Capacidades

    OpenAIRE

    García Fernández, José Manuel; Vicent Juan, Maria; Gonzálvez Macià, Carolina

    2016-01-01

    Este material, centrado en el estudio de las Altas Capacidades, pretende favorecer la adquisición de destrezas en el conocimiento e intervención en el ámbito de la educación especial para conocer de primera mano las diferentes formas que se tienen para actuar e intervenir ante niños con necesidades específicas de apoyo educativo.

  15. Sistema de administración de las capacidades

    OpenAIRE

    Oscar Aguilera Cruz; Jenny Ruiz de la Peña

    2007-01-01

    Realiza un diseño de las líneas de producción de una empresa y se determina si para una fecha determinada existe capacidad productiva disponible para satisfacer una demanda de un cliente. El proceso se vincula al de negociación comercial garantizando que los negociadores de la empresa conozcan en cada momento la situación real de los medios productivos de la empresa.

  16. Capital humano y capacidad humana Human capital and human capacity

    Directory of Open Access Journals (Sweden)

    Sen Amartya Kumar

    1998-12-01

    Full Text Available En este articulo se examinan las relaciones y diferencias entre el concepto de 'capital humano' y el concepto de 'capacidad humana'. El concepto de capital humano es mas limitado puesto que solo concibe las cualidades humanas en su relación con el crecimiento económico mientras que el concepto de capacidades da énfasis a la expansión de libertad humana para vivir el tipo de vida que la gente considera valedera. Cuando se adopta esa visión mas amplia, el proceso de desarrollo no puede verse simplemente como un incremento del PIB sino como la expansión de la capacidad humana para llevar una vida mas libre y mas digna.In this article the relationships and the differences between the concept of 'human capital' and the concept of 'human capability' are examined. The concept of human capital is more limited since it only conceives human qualities in relation to economic growth, whereas the concept of capabili ties puts emphasis on the expansion of human freedom to live the kind of life that people judge valuable. Whenthis broader vision is adopted, the process of development cannot be seen as simply an increase in the GNP, but rather as the expansion of the human capability to live a more free and worthy life.

  17. Reproducibilidad en la lectura de un set de placas de citología cérvico-uterinas en cuatro centros especializados de Medellín, Antioquia / Reproducibility of reading a set of cervical cytology smears in four specialized centers in Medellin, Antioquia

    OpenAIRE

    Edwin Guevara; Armando Baena; Maribel Almonte; Jorge E. Salaza; Ángela Gaviria; Gloria I. Sánchez

    2014-01-01

    Objetivo: evaluar el grado de reproducibilidad en la lectura de placas de citología cérvico-uterina entre cuatro centros de lectura especializados de la ciudad de Medellín, Colombia. Metodología: 181 placas de citología cérvico-uterina provenientes de un estudio de prevalencia de anormalidades citológicas en Pueblorrico, Antioquia, se sometieron a lectura en cuatro centros especializados de Medellín. Se le pidió a cada centro que realizara una lectura rutinaria man...

  18. Diffusion-weighted MRI 3T nella valutazione di pazienti con carcinoma dell'endometrio e della cervice uterina: confronto con FDG PET-TC con mezzo di contrasto iodato

    OpenAIRE

    Pizzuti, Laura Micol

    2010-01-01

    In questo studio ho valutato 40 pazienti con carcinoma dell'endometrio e della cervice uterina sottoponendole ad esame RM con mdc ev (gadolinio) con l'aggiunta di sequenze di diffusione (DWI) ed ad esame PET-TC con mdc iodato. Ho poi dimostrato la correlazione tra i valori delle mappe di ADCmin., che si ottengono dalle sequenze DWI ed i valori delle SUVmax che misurano l'iperaccumulo di FDG nel sito tumorale. Nel lavoro ho notato una correlazione inversa che esiste tra questi due parametri ch...

  19. Transfusão intra-uterina em fetos afetados pela doença hemolítica perinatal grave: um estudo descritivo Intrauterine transfusion in fetuses affected by severe perinatal hemolytic disease: a descriptive study

    OpenAIRE

    Dorival Antônio Vitorello; Luiz Miguel Mitri Parente; Rodolfo João Ramos; Luís Flávio de Andrade Gonçalves; Cláudia Diniz Baumgarten; Jorge Abi Saab Neto

    1998-01-01

    Objetivo: analisar 54 transfusões intravasculares intra-uterinas (TIVs), ressaltando complicações do procedimento e morbimortalidade perinatal. Material e Métodos: fetos submetidos a TIVs na Clínica Materno-Fetal e Maternidade Carmela Dutra (Florianópolis, SC), entre janeiro de 1992 e agosto de 1997, foram incluídos no estudo. As características das gestantes, dados relativos ao procedimento e ao recém-nascido foram tabulados para análise e apresentados de forma descritiva, utilizando-se perc...

  20. Capacidade antioxidante de frutas Antioxidant capacity of the fruits

    Directory of Open Access Journals (Sweden)

    Enayde de Almeida Melo

    2008-06-01

    Full Text Available Extratos aquoso e acetônico de 15 frutas foram submetidos a ensaios para investigar a habilidade de seqüestrar o radical estável 1,1-difenil-2-picrilhidrazil (DPPH e a capacidade de inibir a oxidação em sistema modelo β-caroteno/ácido linoléico. Todas as frutas exibiram propriedade antioxidante, entretanto a ação foi diferenciada entre elas. O extrato aquoso da acerola, caju, mamão "Formosa", mamão Havaí, laranja pêra e goiaba foram os mais eficazes (superior a 70%, enquanto que o do abacaxi, laranja cravo, manga rosa, melão espanhol, melão japonês, melão orange flesh e pinha apresentaram ação moderada (60-70% e o da manga espada e melancia exibiram a mais fraca capacidade de seqüestrar o radical DPPH. Os extratos acetônico da acerola, caju, pinha e goiaba exibiram uma forte capacidade de seqüestrar o radical DPPH (superior a 70%. Em sistema modelo β-caroteno/ácido linoléico, o extrato aquoso da pinha e o acetônico da goiaba exibiram moderada capacidade antioxidante (60-70% enquanto que a acerola (extrato aquoso e o mamão formosa (extrato acetônico os menores percentuais. Frente à capacidade antioxidante exibida, as frutas podem ser apontadas como fontes de antioxidantes naturais, destacando-se a acerola, caju, mamão Formosa, mamão Havaí, goiaba, laranja pêra, e a pinha por terem apresentado uma potente capacidade antioxidante.Aqueous and acetone extracts from 15 fruits have been screened for antioxidant activity using DPPH method and ß-carotene/linoleic acid model. All fruits studied showed antioxidant activity, but in different extent. Acerola, cashew-apple, papaya "formosa", papaya "solo", orange and guava showed the higher antioxidant activity (>70% in DPPH method, followed by pineapple, bergamont, mango "rosa", melon "reticulares", melon "inodorus", melon "orange flesh" and sugar-apple aqueous extract (moderate, 60-70% and mango "espada" and watermelon aqueous extract, the lowest activity. Acerola, cashew

  1. Estudio macro-microscópico de la involución uterina postparto en alpacas - Macro and microscopic of uterine involution postpartum in alpacas

    Directory of Open Access Journals (Sweden)

    MVZ. Augusto Excelmes Loza. Puno-Perú 2005

    2006-07-01

    Full Text Available El presente estudio se llevo a cabo en el Laboratorio de Histología y Embriología de la Facultad de Medicina Veterinaria y Zootecnia de la Universidad Nacional del Altiplano; con alpacas provenientes de la Rural Alianza E.P.S. (4,140m.s.n.m, Centro Experimental Quinsachata-INIA (4,050 m.s.n.m y el Centro de Investigación y Producción La Raya (4,130 m.s.n.m, cuyo objetivo fue el estudio del proceso de la involución uterina post parto en la alpaca, desde el parto hasta los 30dpp. Para tal propósito se estudiaron 34 alpacas multíparas de 4 a 6 años de edad. Los resultados fueron: Macroscópicamente: la perdida de peso del útero fue de: 71.07% (385,25g, 25.54% (138.55g y 3.42% (18.6g al primer, segundo y tercer periodo de la involución uterina. La reducción de Peso del cuerno uterino derecho fue de: 55.26% (37.55g, 38.63% (26.25g y 6.11% (4.15g al primer segundo y tercer periodo respectivamente. La secuencia de la perdida de peso del cuerno uterino izquierdo fue de: 75.50% (159.5g, 19.58% (41.25g, y 4.92% (10.40g al primer, segundo y tercer periodo sucesivamente. El descenso de Peso del cuerpo del útero fue de: 76.60% (87.25g, 17.08% (19.45g y 6.32% (7.2g al primer, segundo y tercer periodo sucesivamente. El Peso de reducción gradual de la cervix fue de: 68% (47,15g, 26,10% (18,00g y 5,51% (3,8g al primer, segundo y tercer periodo respectivamente. This study was carried out in the Histology and Embriology laboratory at the Medicine Veterinary and Zootecny of the Altiplano National University, with alpacas coming from the EPS Rural Alliance (4140 m.a.s.l., Quimsachata Research Center (4050 m.a.s.l. and the Raya Research Center (4130 m.a.s.l. whose objective was the study the process of the uterine involution after parturition from day 0 to 30. For such purpose 34 multiparous alpacas from 4 to 6 year old were selectec. The results were: Macroscopically the uterusweight lost 71,07% (385,25g, 25,54% (138,55g and 3,42% (18,6g at first

  2. Estudio macro-microscópico de la involución uterina postparto en Alpacas (Macro and microscopic of uterine involution postpartum in alpacas summary

    Directory of Open Access Journals (Sweden)

    MVZ. Augusto Excelmes Loza.

    2006-04-01

    Full Text Available El presente estudio se llevo a cabo en el Laboratorio de Histología y Embriología de la Facultad de Medicina Veterinaria y Zootecnia de la Universidad Nacional del Altiplano; con alpacas provenientes de la Rural Alianza E.P.S. (4,140m.s.n.m, Centro Experimental Quinsachata-INIA (4,050 m.s.n.m y el Centro de Investigación y Producción La Raya (4,130 m.s.n.m, cuyo objetivo fue el estudio del proceso de la involución uterina post parto en la alpaca, desde el parto hasta los 30dpp. Para tal propósito se estudiaron 34 alpacas multíparas de 4 a 6 años de edad. Los resultados fueron: Macroscópicamente: la perdida de peso del útero fue de: 71.07% (385,25g, 25.54% (138.55g y 3.42% (18.6g al primer, segundo y tercer periodo de la involución uterina. La reducción de Peso del cuerno uterino derecho fue de: 55.26% (37.55g, 38.63% (26.25g y 6.11% (4.15g al primer segundo y tercer periodo respectivamente. La secuencia de la perdida de peso del cuerno uterino izquierdo fue de: 75.50% (159.5g, 19.58% (41.25g, y 4.92% (10.40g al primer, segundo y tercer periodo sucesivamente. El descenso de Peso del cuerpo del útero fue de: 76.60% (87.25g, 17.08% (19.45g y 6.32% (7.2g al primer, segundo y tercer periodo sucesivamente. El Peso de reducción gradual de la cervix fue de: 68% (47,15g, 26,10% 18,00g y 5,51% (3,8g al primer, segundo y tercer periodo respectivamente. Histológicamente: durante los primeros días la superficie uterina es irregular y presenta proyecciones endometriales de aspecto fungiforme y esta cubierto por células pavimentosas, estroma con la presencia de linfocitos, macrófagos y fibroblastos, glándulas reducidas en su diámetro. A partir del sexto día, muestra cambio en el regenerativo, el epitelio de hace predominantemente cúbico, Del sexto al doceavo día el epitelio continua irregular con pequeñas áreas con células epiteliales pavimentosas y cúbicas, lo cual coincide con el cambio en el peso del útero en forma significativa

  3. Acurácia da histeroscopia na avaliação da cavidade uterina em pacientes com sangramento uterino pós-menopausa

    Directory of Open Access Journals (Sweden)

    Machado Maria Karenina N.

    2003-01-01

    Full Text Available OBJETIVO: investigar a acurácia da histeroscopia como método de estudo da cavidade uterina de pacientes com sangramento na pós-menopausa. MÉTODO: trata-se de estudo transversal, que consistiu na avaliação de 78 prontuários de pacientes menopausadas com queixa de sangramento, no período de janeiro de 2000 a junho de 2002 no Centro Estadual de Oncologia do Estado da Bahia, submetidas à histeroscopia com biópsia de endométrio. Os achados histeroscópicos foram classificados como benignos (miomas, pólipos, atrofia, normal e suspeitos (espessamento, hiperplasias e câncer e os achados histopatológicos como benignos (miomas, pólipos, atrofia, hiperplasia típica e malignos (hiperplasia atípica e câncer. Os resultados da histeroscopia foram comparados com os exames histopatológicos. RESULTADOS: em relação aos achados suspeitos (espessamento, hiperplasias e câncer, a sensibilidade da histeroscopia foi de 85,7% e a especificidade de 88,7%, quando comparados à histopatologia. O valor preditivo positivo foi de 42,8% e o negativo 98,4%. A razão de probabilidades do teste positivo e a razão de probabilidades do teste negativo foram respectivamente 7,6 e 0,16. A acuidade da histeroscopia foi de 88,4% e o índice kappa 0,5. CONCLUSÃO: a histeroscopia isoladamente não apresentou, no presente estudo, acurácia aceitável, reforçando o conceito de que sua principal vantagem é dirigir a biópsia, devendo sempre estar associada ao diagnóstico histopatológico.

  4. Capacidad funcional y salud: orientaciones para cuidar al adulto mayor

    Directory of Open Access Journals (Sweden)

    CLARA INÉS GIRALDO M

    2008-07-01

    Full Text Available Objetivo: valorar la salud y la capacidad funcional de ancianos con dependencia funcional para el autocuidado, como elementos para orientar el cuidado de enfermería y el cuidado familiar en casa. Metodología: estudio descriptivo transversal en 40 personas de 65 y más años con dependencia funcional, cuidados por familiar, en Envigado Colombia; muestreo por conveniencia. Valoración de la capacidad funcional con el índice de Katz y la escala de Lawton y Brody, ajustados según grupo de Neurociencias, Universidad de Antioquia. Hallazgos: población entre 67 y 98 años, edad promedio 84,08 años; 82,5% mujeres. Sistemas orgánicos más afectados: cardiovascular, osteomuscular y neurológico; entre 47,5% y 27,5% de los ancianos los tenían comprometidos. Se identificaron dos comportamientos: los hombres, y las personas de edad más avanzada, con mayores grados de dependencia en las actividades básicas e instrumentales; no se determinó asociación estadística por el pequeño tamaño muestral. Las actividades básicas estaban afectadas así: entre el 67,5% y el 55% de los ancianos requería ayuda; y las actividades instrumentales estuvieron comprometidas: entre el 95% y el 75% de los ancianos son totalmente dependientes en ellas. Conclusiones: Enfermería ha de considerar la valoración de la capacidad funcional articulada al estado de salud integral de los ancianos, para orientar su cuidado y apoyar a los cuida-dores familiares. Esto además previene dependencias de cuidado innecesarias que estarían en contra de las aspiraciones del adulto mayor como individuo autónomo e independiente. Es tarea de profesionales en enfermería capacitar a cuidadores familiares sobre valoración de la capacidad funcional.

  5. Las capacidades financieras de la población colombiana

    OpenAIRE

    2012-01-01

    La inclusión financiera se ha convertido en un tema cada vez más relevante dentro de la agenda pública del país, y con esto el diseño de iniciativas privadas y públicas para fomentarla. El presente documento está fundamentado en la tesis de que para hacer estas políticas más eficientes y eficaces, es necesario contar con buenas mediciones de las capacidades financieras de la población, las cuales no se limitan exclusivamente al conocimiento de temas financieros, sino que abarcan, en un sentid...

  6. Proyecto de Historia como respuesta educativa a las altas capacidades

    OpenAIRE

    Miguel Yoldi, Yohanna

    2014-01-01

    Este trabajo presenta una propuesta de intervención en el aula que responde a las necesidades educativas del alumnado con altas capacidades, desde una escuela inclusiva. Para realizarlo, nos hemos basado en dos autores: Roger J. Sternberg y Joseph S. Renzulli, que no sólo definen las características y necesidades educativas de este colectivo, sino que también proponen posibles respuestas que respondan a su forma de aprender. Desde el área de Ciencias Sociales, apostamos por el aprendizaje bas...

  7. Todos iguales, todos diferentes. Altas capacidades en el aula.

    OpenAIRE

    Soloaga Martín, Beatriz

    2012-01-01

    El presente trabajo se centra en el alumnado de altas capacidades y las posibilidades de atención en el marco del modelo de escuela inclusiva. Se comienza explicando las características de estos alumnos, los estereotipos que existen sobre ellos y las disincronías que es frecuente encontrar en su desarrollo. Se sigue la clasificación de varios autores para distinguir entre superdotación y talento viendo las diferencias significativas que existen entre ellos. De forma breve se ha...

  8. La capacidad directiva clave del éxito empresarial

    OpenAIRE

    García Echevarría, Santiago

    2007-01-01

    La orientación a resultados constituye antropológica y éticamente la clave que debe regir el comportamiento humano. Los costes son, por muy relevantes que sean, su dimensión instrumental. La capacidad de dirigir, la definición del sistema de dirección de una empresa, constituye el verdadero intangible de una empresa, de una institución, lo que descansa en el sistema de valores que rige tanto el comportamiento directivo como la orientación interna y externa de la corporación. La...

  9. Capacidade antioxidante de frutas Antioxidant capacity of the fruits

    OpenAIRE

    Enayde de Almeida Melo; Maria Inês Sucupira Maciel; Vera Lúcia Arroxelas Galvão de Lima; Rosilda Josefa do Nascimento

    2008-01-01

    Extratos aquoso e acetônico de 15 frutas foram submetidos a ensaios para investigar a habilidade de seqüestrar o radical estável 1,1-difenil-2-picrilhidrazil (DPPH) e a capacidade de inibir a oxidação em sistema modelo β-caroteno/ácido linoléico. Todas as frutas exibiram propriedade antioxidante, entretanto a ação foi diferenciada entre elas. O extrato aquoso da acerola, caju, mamão "Formosa", mamão Havaí, laranja pêra e goiaba foram os mais eficazes (superior a 70%), enquanto que o do a...

  10. Protrusive and Contractile Forces of Spreading Human Neutrophils.

    Science.gov (United States)

    Henry, Steven J; Chen, Christopher S; Crocker, John C; Hammer, Daniel A

    2015-08-18

    Human neutrophils are mediators of innate immunity and undergo dramatic shape changes at all stages of their functional life cycle. In this work, we quantified the forces associated with a neutrophil's morphological transition from a nonadherent, quiescent sphere to its adherent and spread state. We did this by tracking, with high spatial and temporal resolution, the cell's mechanical behavior during spreading on microfabricated post-array detectors printed with the extracellular matrix protein fibronectin. Two dominant mechanical regimes were observed: transient protrusion and steady-state contraction. During spreading, a wave of protrusive force (75 ± 8 pN/post) propagates radially outward from the cell center at a speed of 206 ± 28 nm/s. Once completed, the cells enter a sustained contractile state. Although post engagement during contraction was continuously varying, posts within the core of the contact zone were less contractile (-20 ± 10 pN/post) than those residing at the geometric perimeter (-106 ± 10 pN/post). The magnitude of the protrusive force was found to be unchanged in response to cytoskeletal inhibitors of lamellipodium formation and myosin II-mediated contractility. However, cytochalasin B, known to reduce cortical tension in neutrophils, slowed spreading velocity (61 ± 37 nm/s) without significantly reducing protrusive force. Relaxation of the actin cortical shell was a prerequisite for spreading on post arrays as demonstrated by stiffening in response to jasplakinolide and the abrogation of spreading. ROCK and myosin II inhibition reduced long-term contractility. Function blocking antibody studies revealed haptokinetic spreading was induced by β2 integrin ligation. Neutrophils were found to moderately invaginate the post arrays to a depth of ∼1 μm as measured from spinning disk confocal microscopy. Our work suggests a competition of adhesion energy, cortical tension, and the relaxation of cortical tension is at play at the onset of

  11. Dynamic regulation of β1 subunit trafficking controls vascular contractility

    OpenAIRE

    Leo, M. Dennis; Bannister, John P.; Narayanan, Damodaran; Nair, Anitha; Grubbs, Jordan E.; Gabrick, Kyle S.; Boop, Frederick A.; Jaggar, Jonathan H.

    2014-01-01

    Plasma membrane ion channels composed of pore-forming and auxiliary subunits regulate physiological functions in virtually all cell types. A conventional view is that ion channels assemble with their auxiliary subunits prior to surface trafficking of the multiprotein complex. Arterial myocytes express large-conductance Ca2+-activated potassium (BK) channel α and auxiliary β1 subunits that modulate contractility and blood pressure and flow. The data here show that although most BKα subunits ar...

  12. High-throughput screening for modulators of cellular contractile force

    OpenAIRE

    Park, Chan Young; Zhou, Enhua H; Tambe, Dhananjay; Chen, Bohao; Lavoie, Tera; Dowell, Maria; Simeonov, Anton; Maloney, David J.; Marinkovic, Aleksandar; Tschumperlin, Daniel J.; Burger, Stephanie; Frykenberg, Matthew; Butler, James P.; Stamer, W. Daniel; Johnson, Mark

    2014-01-01

    When cellular contractile forces are central to pathophysiology, these forces comprise a logical target of therapy. Nevertheless, existing high-throughput screens are limited to upstream signaling intermediates with poorly defined relationship to such a physiological endpoint. Using cellular force as the target, here we screened libraries to identify novel drug candidates in the case of human airway smooth muscle cells in the context of asthma, and also in the case of Schlemm's canal endothel...

  13. Carbon monoxide affects electrical and contractile activity of rat myocardium

    OpenAIRE

    Porokhnya Maria V; Haertdinov Nail N; Abramochkin Denis V; Zefirov Andrew L; Sitdikova Gusel F

    2011-01-01

    Abstract Background Carbon monoxide (CO) is a toxic gas, which also acts in the organism as a neurotransmitter. It is generated as a by-product of heme breakdown catalyzed by heme oxygenase. We have investigated changes in electrical and contractile activity of isolated rat atrial and ventricular myocardium preparations under the influence of CO. Methods Standard microelectrode technique was used for intracellular registration of electrical activity in isolated preparations of atrial and vent...

  14. American Ginseng Acutely Regulates Contractile Function of Rat Heart

    Directory of Open Access Journals (Sweden)

    Mao eJiang

    2014-03-01

    Full Text Available Chronic ginseng treatments have been purported to improve cardiac performance. However reports of acute administration of ginseng on cardiovascular function remain controversial and potential mechanisms are not clear. In this study, we examined effects of acute North American ginseng (Panax quinquefolius administration on rat cardiac contractile function by using electrocardiogram (ECG, non-invasive blood pressure measurement (BP and Langendorff isolated, spontaneously beating, perfused heart measurements (LP. Eight-week old male Sprague Dawley rats (n= 8 per group were gavaged with a single dose of water-soluble American ginseng at 300 mg/kg body weight. Heart rate and blood pressure were measured prior to and at 1 and 24 hr after gavaging (ECG and BP. Additional groups were used for each time point for Langendorff measurements. Heart rate was significantly decreased (ECG: 1 hr: 6 ± 0.2%, 24 hr: 8 ± 0.3%; BP: 1 hr: 8.8 ± 0.2%, 24 hr: 13 ± 0.4% and LP: 1 hr: 22 ± 0.4%, 24 hr: 19 ± 0.4% in rats treated with water-soluble ginseng compared with pre or control measures. An initial marked decrease in left ventricular developed pressure was observed in LP hearts but blood pressure changes were not observed in BP group. A direct inhibitory effect of North American ginseng was observed on cardiac contractile function in LP rats and on fluorescence measurement of intracellular calcium transient in freshly isolated cardiac myocytes when exposed to ginseng (1 µg/ml and 10 µg/ml. Collectively these data present evidence of depressed cardiac contractile function by acute administration of North American ginseng in rat. This acute reduction in cardiac contractile function appears to be intrinsic to the myocardium.

  15. A relationship between ultrasonic integrated backscatter and myocardial contractile function.

    OpenAIRE

    Wickline, S. A.; Thomas, L J; Miller, J.G.; Sobel, B E; J. E. Perez

    1985-01-01

    We have shown previously that the physiologic, mechanical cardiac cycle is associated with a parallel, cardiac cycle-dependent variation of integrated backscatter (IB). However, the mechanisms responsible are not known. The mathematical and physiological considerations explored in the present study suggest that the relationship between backscatter and myocardial contractile function reflects cyclic alterations in myofibrillar elastic parameters, with the juxtaposition of intracellular and ext...

  16. Recovery in skeletal muscle contractile function after prolonged hindlimb immobilization

    Science.gov (United States)

    Fitts, R. H.; Brimmer, C. J.

    1985-01-01

    The effect of three-month hindlimb immobilization (IM) in rats on contractile properties of slow-twitch soleus (SOL), fast-twitch extensor digitorum longus, and fast-twitch superficial region of the vastus lateralis were measured after 0, 14, 28, 60, and 90 days of recovery on excized, horizontally suspended muscles stimulated electrically to maximal twitch tension. IM caused decreases in muscle-to-body weight ratios for all muscles, with no complete recovery even after 90 days. The contractile properties of the fast-twitch muscles were less affected by IM than those of the slow-twitch SOL. The SOL isometric twitch duration was shortened, due to reduced contraction and half-relaxation time, both of which returned to control levels after 14 days of recovery. The peak tetanic tension, P(O), g/sq cm,, decreased with IM by 46 percent in the SOL, but recovered by the 28th day. The maximum shortening velocity was not altered by IM in any of the muscles. Thus, normal contractile function could recover after prolonged limb IM.

  17. Capacidade combinatoria de capim elefante com base em caracteres morfoagronomicos

    Directory of Open Access Journals (Sweden)

    Vanessa Quitete Ribeiro da Silva

    2014-01-01

    Full Text Available Devido ao grande potencial de produção de biomassa do capim elefante, objetivou-se neste trabalho avaliar genitores para geração de híbridos superiores. Para tanto, foram estimados os efeitos da capacidade geral de combinação (CGC dos genitores e da capacidade específica de combinação (CEC de híbridos de capim elefante por meio de análise dialélica adaptada a dialelos parciais. Foram avaliados 16 híbridos e oito genitores utilizando delineamento em blocos casualizados com três repetições. Verificou-se a presença de variabilidade genética entre os híbridos e genitores, com predominância do efeito gênico de dominância para a maioria das características. De forma geral, os melhores resultados na CGC são os genitores Taiwan A-144, Vruckwona Africana e Taiwan A-146. Os melhores cruzamentos com base na CEC são Taiwan A-144 x Taiwan A-146 e Pusa Napier nº2 x Mercker Santa Rita.

  18. Capacidad de resistencia, vulnerabilidad y cultura de riesgos

    Directory of Open Access Journals (Sweden)

    Alfredo Cilento Sarli

    2005-01-01

    Full Text Available La ocurrencia de cataclismos como sismos, aludes, tsunamis, huracanes, entre otros, constituyen amenazas naturales para los seres vivos, pero los seres humanos son los que convierten las amenazas en contingencias desastrosas. Por ello, la lucha contra los desastres provocados por los hombres y mujeres que habitan el planeta, es la batalla por el logro de una cultura de seguridad y prevención, respetuosa del medio ambiente natural y de la calidad del medio construido. Esa cultura deriva de la capacidad de resistencia de la población, y de una adecuada educación y preparación para enfrentar las contingencias antes de que ocurran, durante su ocurrencia y después de ocurridas. El fin determinante es el de salvar vidas y propiedades, y recuperar las facilidades para la vida y el trabajo, corrigiendo las fallas y vicios existentes antes de ocurrida la contingencia. En el desarrollo de esa cultura de seguridad el papel de las comunidades educativas, académicas y científicas es preponderante, al igual que la justa evaluación de la capacidad de resistencia de la sociedad, como elemento clave en los estudios de vulnerabilidad de los asentamientos humanos. En este trabajo se plantean algunos de los esfuerzos atribuibles a los actores mencionados, particularmente a las universidades públicas, dentro del marco de la Década de la Educación para el Desarrollo Sostenible 2005-2014, designada por Naciones Unidas.

  19. Altas capacidades intelectuales. Diagnóstico y seguimiento de una NEE.

    OpenAIRE

    Pérez-i-Martra, Pep

    2013-01-01

    Según la OMS, el 2,3% de la población mundial tiene altas capacidades intelectuales, es decir, en la vida académica de un profesor, éste tendría que descubrir una media de 40 alumnos con capacidades superiores a la media. Saber en qué consisten las altas capacidades y hacer un pre diagnóstico de las mismas será, en muchas ocasiones, trabajo del profesor de secundaria. Pero, ¿tiene éste conocimientos para percatarse de la presencia en clase de un alumno con altas capacidades? ¿Y una vez estudi...

  20. Multiple mechanisms involved in oxytocin-induced modulation of myometrial contractility

    Institute of Scientific and Technical Information of China (English)

    Anatoly SHMYGOL; Joanna GULLAM; Andrew BLANKS; Steven THORNTON

    2006-01-01

    Oxytocin is a small peptide hormone with multiple sites of action in human body.It regulates a large number of reproduction-related processes in all species.Particularly important is its ability to stimulate uterine contractility.This is achieved by multiple mechanisms involving sarcoplasmic reticulum Ca2+ release and sensitization of the contractile apparatus to Ca2+.In this paper,we review the data published by US and other groups on oxytocin-induced modulation of uterine contractility.We conclude that sensitization of contractile apparatus to Ca2+ is the most relevant physiological effect of oxytocin on human myometrium.

  1. Alterações na gasometria de fetos aloimunizados após procedimento de transfusão intra-uterina

    Directory of Open Access Journals (Sweden)

    Nomura Roseli Mieko Yamamoto

    2003-01-01

    Full Text Available OBJETIVO: este estudo, realizado em gestações com aloimunização pelo fator Rh, tem como objetivo descrever as alterações gasométricas e do equilíbrio ácido-básico fetal antes e após transfusões intra-uterinas (TIU. MÉTODO: no período de junho de 2001 a outubro de 2001, antes e após a TIU em fetos de gestantes aloimunizadas, foram avaliados prospectivamente a gasometria e o equilíbrio ácido-básico no sangue da veia umbilical. As medidas foram realizadas em 8 amostras de sangue de 5 fetos. O sangue fetal foi obtido por cordocentese da veia umbilical antes e após TIU. Os resultados obtidos foram comparados com a expansão volêmica na TIU, a idade gestacional no procedimento, o peso fetal estimado pela ultra-sonografia e as variações da hemoglobina fetal (g/dL. RESULTADOS: em todos os casos foi observada queda nos valores do pH, com redução média de 0,09 (DP=0,02. A hemoglobina fetal apresentou aumento médio de 8,4 g/dL (DP=2,9 g/dL. Foi constatada também variação negativa da pO2 (média = -1,28 mmHg na concentração de HCO3_ (média = _2,25 mEq/l. Houve aumento da pCO2 (média = 3,2 mmHg e redução nos valores do excesso de bases (média = -3,75. CONCLUSÃO: a análise das gasometrias permite concluir que o procedimento de TIU acompanha-se de queda nos valores do pH de sangue de veia umbilical, demonstrando haver acidemia fetal relativa após o procedimento.

  2. Human Engineered Heart Tissue: Analysis of Contractile Force

    OpenAIRE

    Ingra Mannhardt; Kaja Breckwoldt; David Letuffe-Brenière; Sebastian Schaaf; Herbert Schulz; Christiane Neuber; Anika Benzin; Tessa Werner; Alexandra Eder; Thomas Schulze; Birgit Klampe; Torsten Christ; Marc N. Hirt; Norbert Huebner; Alessandra Moretti1

    2016-01-01

    Analyzing contractile force, the most important and best understood function of cardiomyocytes in vivo is not established in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM). This study describes the generation of 3D, strip-format, force-generating engineered heart tissues (EHT) from hiPSC-CM and their physiological and pharmacological properties. CM were differentiated from hiPSC by a growth factor-based three-stage protocol. EHTs were generated and analyzed histological...

  3. Human engineered heart tissue: Analysis of contractile force

    OpenAIRE

    Mannhardt, I.; Breckwoldt, K.; Letuffe-Breniere, D.; Schaaf, S. van der; H. Schulz; Neuber, C.; Benzin, A.; Werner, T; Eder, A.; Schulze, T.; Klampe, B.; Christ, T; Hirt, M.N.; Huebner, N; Moretti, A.

    2016-01-01

    Analyzing contractile force, the most important and best understood function of cardiomyocytes in vivo is not established in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM). This study describes the generation of 3D, strip-format, force-generating engineered heart tissues (EHT) from hiPSC-CM and their physiological and pharmacological properties. CM were differentiated from hiPSC by a growth factor-based three-stage protocol. EHTs were generated and analyzed histological...

  4. Effect of serotonin on small intestinal contractility in healthy volunteers

    DEFF Research Database (Denmark)

    Hansen, M.B.; Arif, F.; Gregersen, H.; Bruusgaard, H.; Wallin, L.

    2008-01-01

    -lived adverse effects following intraluminal serotonin stimulations. We conclude that exogenous serotonin in the lumen of the upper part of the small intestine does not seem to change antro-duodeno-jejunal contractility significantly in healthy adult volunteers Udgivelsesdato: 2008......The physiological significance of serotonin released into the intestinal lumen for the regulation of motility is unknown in humans. The aim of this study was to evaluate the effect of serotonin infused into the lumen of the gastric antrum, duodenum or the jejunum, on antro...

  5. Frequência de mola hidatiforme em tecidos obtidos por curetagem uterina Frequency of hydatidiform mole in tissue obtained by curettage

    Directory of Open Access Journals (Sweden)

    Andressa Biscaro

    2012-06-01

    Full Text Available OBJETIVO: Determinar a frequência de mola hidatiforme em tecidos obtidos por curetagem uterina. MÉTODOS: Estudo transversal, prospectivo e descritivo que incluiu pacientes submetidas à curetagem uterina por diagnóstico de aborto ou mola hidatiforme cujo material obtido foi encaminhado para exame anatomopatológico. Foram excluídas aquelas que não aceitaram participar da pesquisa, recusando-se a assinar o Termo de Consentimento Informado Livre e Esclarecido. Foram analisadas as seguintes variáveis: achados anatomopatológicos, idade, raça/cor, número de gestações e abortos prévios, idade gestacional no momento do diagnóstico, níveis séricos quantitativos da fração beta da gonadotrofina coriônica humana e achados ultrassonográficos. As variáveis foram empregadas para a verificação com o diagnóstico histológico, considerado o padrão-ouro. Os dados foram armazenados e analisados no software Microsoft Excel® e no programa Epi-Info, versão 6.0 (STATCALC e os resultados apresentados como frequência (porcentagem ou média±desvio padrão. Para a associação entre variáveis qualitativas foi usado o teste do χ², e admitiu-se significância estatística quando pPURPOSE: To determine the frequency of hydatiform mole in tissues obtained by curettage. METHODS: A cross-sectional, prospective and descriptive conducted on patients who underwent curretage due to a diagnosis of abortion or hydatiform mole whose material was sent for pathological examination. We excluded women who did not accept to participate and refused to sign the free informed consent form. We studied the following variables: pathological findings, age, race, number of pregnancies and previous abortions, gestational age at diagnosis, quantitative serum beta fraction of human chorionic gonadotropin and ultrasound findings. The data were compared to the to histological diagnosis, considered to be the gold standard. Data were stored and analyzed in Microsoft Excel

  6. Expresi??n de ant??genos HLA clase I e infecci??n por papilomavirus humano tipo 16 en la neoplasia cervical uterina y lesiones precursoras

    OpenAIRE

    Torres Garc??a, Luis Manuel

    1992-01-01

    Sobre una casu??stica de 109 diagn??sticos de diferentes entidades nosologicas de cervix uterino, se determina la presencia de ant??genos hla clase i mediante la t??cnica de la inmunoperoxidasa, encontrando que tanto el epitelio normal como los condilomas y las lesiones intraepiteliales conservan la expresi??n de estos ant??genos, mientras que los tumores invasores pierden esta capacidad en el 15% de los casos, correlacion??ndose con la invasi??n del estroma y un aumento de su malignidad. Se ...

  7. A viscous two-phase model for contractile actomyosin bundles.

    Science.gov (United States)

    Oelz, Dietmar

    2014-06-01

    A mathematical model in one dimension for a non-sarcomeric actomyosin bundle featuring anti-parallel flows of anti-parallel F-actin is introduced. The model is able to relate these flows to the effect of cross-linking and bundling proteins, to the forces due to myosin-II filaments and to external forces at the extreme tips of the bundle. The modeling is based on a coarse graining approach starting with a microscopic model which includes the description of chemical bonds as elastic springs and the force contribution of myosin filaments. In a second step we consider the asymptotic regime where the filament lengths are small compared to the overall bundle length and restrict to the lowest order contributions. There it becomes apparent that myosin filaments generate forces which are partly compensated by drag forces due to cross-linking proteins. The remaining local contractile forces are then propagated to the tips of the bundle by the viscosity effect of bundling proteins in the filament gel. The model is able to explain how a disordered bundle of comparatively short actin filaments interspersed with myosin filaments can effectively contract the two tips of the actomyosin bundle. It gives a quantitative description of these forces and of the anti-parallel flows of the two phases of anti-parallel F-actin. An asymptotic version of the model with infinite viscosity can be solved explicitly and yields an upper bound to the contractile force of the bundle. PMID:23670678

  8. Mechanisms underlying the impaired contractility of diabetic cardiomyopathy

    Institute of Scientific and Technical Information of China (English)

    Marie-Louise; Ward; David; J; Crossman

    2014-01-01

    Cardiac dysfunction is a well-known consequence of diabetes,with sustained hyperglycaemia leading to the development of a cardiomyopathy that is independent of cardiovascular disease or hypertension.Animal models of diabetes are commonly used to study the pathophysiology of diabetic cardiomyopathy,with the hope that increased knowledge will lead ultimately to better therapeutic strategies being developed.At physiological temperature,left ventricular trabeculae isolated from the streptozotocin rat model of type 1 diabetes showed decreased stress and prolonged relaxation,but with no evidence that decreased contractility was a result of altered myocardial Ca2+handling.Although sarcoplasmic reticulum(SR)Ca2+reuptake appeared slower in diabetic trabeculae,it was offset by an increase in actionpotential duration,thereby maintaining SR Ca2+content and favouring increased contraction force.Frequency analysis of t-tubule distribution by confocal imaging of ventricular tissue labeled with wheat germ agglutinin or ryanodine receptor antibodies showed a reduced T-power for diabetic tissue,but the differences were minor in comparison to other models of heart failure.The contractile dysfunction appeared to be the result of disrupted F-actin in conjunction with the increased typeⅠcollagen,with decreased myofilament Ca2+sensitivity contributing to the slowed relaxation.

  9. Human Engineered Heart Tissue: Analysis of Contractile Force.

    Science.gov (United States)

    Mannhardt, Ingra; Breckwoldt, Kaja; Letuffe-Brenière, David; Schaaf, Sebastian; Schulz, Herbert; Neuber, Christiane; Benzin, Anika; Werner, Tessa; Eder, Alexandra; Schulze, Thomas; Klampe, Birgit; Christ, Torsten; Hirt, Marc N; Huebner, Norbert; Moretti, Alessandra; Eschenhagen, Thomas; Hansen, Arne

    2016-07-12

    Analyzing contractile force, the most important and best understood function of cardiomyocytes in vivo is not established in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM). This study describes the generation of 3D, strip-format, force-generating engineered heart tissues (EHT) from hiPSC-CM and their physiological and pharmacological properties. CM were differentiated from hiPSC by a growth factor-based three-stage protocol. EHTs were generated and analyzed histologically and functionally. HiPSC-CM in EHTs showed well-developed sarcomeric organization and alignment, and frequent mitochondria. Systematic contractility analysis (26 concentration-response curves) reveals that EHTs replicated canonical response to physiological and pharmacological regulators of inotropy, membrane- and calcium-clock mediators of pacemaking, modulators of ion-channel currents, and proarrhythmic compounds with unprecedented precision. The analysis demonstrates a high degree of similarity between hiPSC-CM in EHT format and native human heart tissue, indicating that human EHTs are useful for preclinical drug testing and disease modeling. PMID:27211213

  10. Human Engineered Heart Tissue: Analysis of Contractile Force

    Directory of Open Access Journals (Sweden)

    Ingra Mannhardt

    2016-07-01

    Full Text Available Analyzing contractile force, the most important and best understood function of cardiomyocytes in vivo is not established in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM. This study describes the generation of 3D, strip-format, force-generating engineered heart tissues (EHT from hiPSC-CM and their physiological and pharmacological properties. CM were differentiated from hiPSC by a growth factor-based three-stage protocol. EHTs were generated and analyzed histologically and functionally. HiPSC-CM in EHTs showed well-developed sarcomeric organization and alignment, and frequent mitochondria. Systematic contractility analysis (26 concentration-response curves reveals that EHTs replicated canonical response to physiological and pharmacological regulators of inotropy, membrane- and calcium-clock mediators of pacemaking, modulators of ion-channel currents, and proarrhythmic compounds with unprecedented precision. The analysis demonstrates a high degree of similarity between hiPSC-CM in EHT format and native human heart tissue, indicating that human EHTs are useful for preclinical drug testing and disease modeling.

  11. Resolving the role of actoymyosin contractility in cell microrheology.

    Directory of Open Access Journals (Sweden)

    Christopher M Hale

    Full Text Available Einstein's original description of Brownian motion established a direct relationship between thermally-excited random forces and the transport properties of a submicron particle in a viscous liquid. Recent work based on reconstituted actin filament networks suggests that nonthermal forces driven by the motor protein myosin II can induce large non-equilibrium fluctuations that dominate the motion of particles in cytoskeletal networks. Here, using high-resolution particle tracking, we find that thermal forces, not myosin-induced fluctuating forces, drive the motion of submicron particles embedded in the cytoskeleton of living cells. These results resolve the roles of myosin II and contractile actomyosin structures in the motion of nanoparticles lodged in the cytoplasm, reveal the biphasic mechanical architecture of adherent cells-stiff contractile stress fibers interdigitating in a network at the cell cortex and a soft actin meshwork in the body of the cell, validate the method of particle tracking-microrheology, and reconcile seemingly disparate atomic force microscopy (AFM and particle-tracking microrheology measurements of living cells.

  12. Embolização uterina para tratamento de miomas sintomáticos: impacto na qualidade de vida Uterine embolization for management of symptomatic fibroids: quality-of-life impact

    Directory of Open Access Journals (Sweden)

    Nestor Kisilevzky

    2007-10-01

    Full Text Available OBJETIVO: Verificar a mudança na qualidade de vida de pacientes portadoras de miomatose uterina sintomática submetidas a tratamento por embolização. MATERIAIS E MÉTODOS: Quarenta mulheres portadoras de miomatose uterina sintomática que foram tratadas com a técnica de embolização responderam a um questionário de qualidade de vida antes e 12 semanas após o procedimento. RESULTADOS: Verificou-se que o escore médio relacionado com a gravidade dos sintomas nas 40 pacientes antes da embolização foi de 62,07 ± 6,34 e se modificou, com significância estatística após o tratamento, quando se verificou escore médio de 20,42 ± 3,81. Da mesma forma, comprovou-se a melhora na qualidade de vida pela modificação dos escores antes e depois do tratamento, o que também apresentou significância estatística, passando de 40,26 ± 2,98 para 85,06 ± 2,57. CONCLUSÃO: A embolização uterina provoca alívio evidente dos sintomas relacionados com a miomatose e proporciona melhora substancial da qualidade de vida das pacientes.OBJECTIVE: To determine the impact on the quality of life in patients with symptomatic uterine fibroids submitted to uterine artery embolization. MATERIALS AND METHODS: Forty patients with symptomatic fibroids submitted to embolization have answered a quality-of-life questionnaire before and 12 weeks after the procedure. RESULTS: Mean score for symptoms severity before the procedure was 62.07 ± 6.34 and decreased with statistical significance to 20.42 ± 3.81 after the procedure. Similarly, scores for quality of life have improved from 40.26 ± 2.98 before the procedure to 85.06 ± 2.57 after the procedure, which again was statistically significant. CONCLUSION: Uterine embolization results in evident symptoms relief and significant improvement in the quality of life of patients with symptomatic fibroids.

  13. Effects of lengthening contraction on calcium kinetics and skeletal muscle contractility in humans

    DEFF Research Database (Denmark)

    Nielsen, J S; Madsen, K; Jørgensen, L V;

    2005-01-01

    We have tested the hypothesis that the altered muscle contractility after lengthening contractions (LC) is caused by altered calcium (Ca2+) kinetics.......We have tested the hypothesis that the altered muscle contractility after lengthening contractions (LC) is caused by altered calcium (Ca2+) kinetics....

  14. Transient impairments in single muscle fibre contractile function after prolonged cycling in elite endurance athletes

    DEFF Research Database (Denmark)

    Hvid, L G; Gejl, Kasper Degn; Bech, R D;

    2013-01-01

    Prolonged muscle activity impairs whole-muscle performance and function. However, little is known about the effects of prolonged muscle activity on the contractile function of human single muscle fibres. The purpose of this study was to investigate the effects of prolonged exercise and subsequent...... recovery on the contractile function of single muscle fibres obtained from elite athletes....

  15. Procedures for rat in situ skeletal muscle contractile properties.

    Science.gov (United States)

    MacIntosh, Brian R; Esau, Shane P; Holash, R John; Fletcher, Jared R

    2011-01-01

    There are many circumstances where it is desirable to obtain the contractile response of skeletal muscle under physiological circumstances: normal circulation, intact whole muscle, at body temperature. This includes the study of contractile responses like posttetanic potentiation, staircase and fatigue. Furthermore, the consequences of disease, disuse, injury, training and drug treatment can be of interest. This video demonstrates appropriate procedures to set up and use this valuable muscle preparation. To set up this preparation, the animal must be anesthetized, and the medial gastrocnemius muscle is surgically isolated, with the origin intact. Care must be taken to maintain the blood and nerve supplies. A long section of the sciatic nerve is cleared of connective tissue, and severed proximally. All branches of the distal stump that do not innervate the medial gastrocnemius muscle are severed. The distal nerve stump is inserted into a cuff lined with stainless steel stimulating wires. The calcaneus is severed, leaving a small piece of bone still attached to the Achilles tendon. Sonometric crystals and/or electrodes for electromyography can be inserted. Immobilization by metal probes in the femur and tibia prevents movement of the muscle origin. The Achilles tendon is attached to the force transducer and the loosened skin is pulled up at the sides to form a container that is filled with warmed paraffin oil. The oil distributes heat evenly and minimizes evaporative heat loss. A heat lamp is directed on the muscle, and the muscle and rat are allowed to warm up to 37°C. While it is warming, maximal voltage and optimal length can be determined. These are important initial conditions for any experiment on intact whole muscle. The experiment may include determination of standard contractile properties, like the force-frequency relationship, force-length relationship, and force-velocity relationship. With care in surgical isolation, immobilization of the origin of the

  16. Levo-Carnitine Reduces Oxidative Stress and Improves Contractile Functions of Fast Muscles in Type 2 Diabetic Rats

    OpenAIRE

    Bin Aleem, Shoaib; Mazhar Hussain, Muhammad; Farooq, Yasir

    2013-01-01

    Background: Metabolic derangements in type 2 diabetes mellitus (T2DM) are likely to affect skeletal muscle contractile functions adversely. Levo-carnitine improves muscle contractile functions in healthy humans and rats and corrects metabolic derangements in T2DM. Therefore, it is likely to improve muscle contractile functions in T2DM as well. This study was designed to determine the effect of levo-carnitine on serum levo-carnitine levels, oxidative stress and contractile parameters of fast m...

  17. Capacidade funcional, morbidades e qualidade de vida de idosos

    Directory of Open Access Journals (Sweden)

    Darlene Mara dos Santos Tavares

    2012-01-01

    Full Text Available La investigación tiene como objetivo describir el perfil demográfico, la capacidad funcional y la morbilidad de los ancianos, y verificar la calidad de vida relacionado con el número de incapacidad funcional y la morbilidad. Estudio transversal analítico llevado a cabo con 2.142 personas mayores. Los datos fueron recolectados en el hogar con un instrumento estructurado. El análisis se realizó distribución de frecuencias, F-test de ANOVA y Bonferroni (p<0,05. La mayoría eran mujeres, años, casado, años de escolaridad y los ingresos de un salario mínimo. Predominaron los adultos mayores discapacidades funcionales relacionadas con la movilidad y morbilidad, los problemas de visión más frecuente y la columna vertebral y la hipertensión. Las puntuaciones más altas de calidad de vida para las relaciones sociales y el funcionamiento de los sentidos y el más bajo para su desarrollo físico y la autonomía. El mayor número de incapacidad funcional y la morbilidad asociadas con bajas puntuaciones en la calidad de vida.

  18. Crisis de la capacidad regulativa y flexibilidad del Derecho

    Directory of Open Access Journals (Sweden)

    Ángeles Galiana Saura

    2010-12-01

    Full Text Available RESUMEN. El contexto actual de nuestro ordenamiento jurídico es calificado en muchas ocasiones como de “crisis del Derecho” o de debilitamiento de la capacidad regulativa del Derecho, donde tiene lugar un aumento de la relevancia de los instrumentos jurídicos “flexibles”, también denominados en ocasiones “soft”, capaces de adaptarse a contextos diversos y cambiantes. El presente artículo realiza una aproximación al fenómeno del soft law, a través de algunas de sus manifestaciones en nuestro ordenamiento jurídico y los efectos que comporta su inclusión en el mismo, especialmente respecto al principio de seguridad jurídica. ABSTRACT. The present context of our legal system is often described as “crisis of Law” or as the weakening of the regulatory capacity of the law, where the relevance of “flexible” juridical means, so called because of their capacity to adapt to diverse and changing environments and which are sometimes referred as “soft” too, is increasing. This article makes an approach to the phenomenon of soft law through some of its manifestations and the effects that come from its inclusion in our legal order, specially in regard to the principle of legal certainty.

  19. Papel da Histerossonografia no Estudo da Cavidade Uterina em Pacientes com Sangramento Uterino Anormal Role of Sonohysterography in the Evaluation of the Uterine Cavity in Patients with Abnormal Uterine Bleeding

    Directory of Open Access Journals (Sweden)

    Angélica Lemos Debs Diniz

    2000-06-01

    Full Text Available Objetivo: determinar o papel da histerossonografia na avaliação das anormalidades da cavidade uterina, em pacientes com sangramento uterino anormal, selecionadas previamente pela ultra-sonografia transvaginal. Métodos: foram selecionadas 48 pacientes na menacme e pós-menopausa, portadoras de sangramento uterino anormal e anormalidades da cavidade uterina, diagnosticadas inicialmente pela ultra-sonografia transvaginal. Todas as pacientes se submeteram à histerossonografia e, posteriormente, aos métodos "padrão ouro", a histeroscopia e/ou histerectomia. As histerossonografias foram avaliadas por dois diferentes médicos e os diagnósticos confrontados. Resultados: a histerossonografia mostrou ter alta sensibilidade e especificidade no diagnóstico das patologias benignas da cavidade uterina. Na presença de pólipo a sensibilidade e a especificidade do método foram, respectivamente, de 100 e 97%, seguido do mioma submucoso cuja sensibilidade e especificidade foram, respectivamente, de 83 e 100%. Já na hiperplasia endometrial e no endométrio normal a sensibilidade e especificidade foram de 100%. Diagnosticamos 33 casos de pólipos, 13 casos de miomas submucosos, quatro casos de hiperplasia endometrial e três casos normais na análise dos métodos "padrão ouro". Houve alta correlação entre os diagnósticos dados pelos dois examinadores. Conclusões: a histerossonografia mostrou ser um exame reprodutível, com alta sensibilidade e especificidade no diagnóstico das patologias benignas da cavidade uterina em pacientes com sangramento uterino anormal.Purpose: to determine the role of sonohysterography in the evaluation of abnormalities in the uterine cavity in patients presenting abnormal uterine bleeding, who had previously been selected by transvaginal ultrasonography. Methods: forty-eight patients presenting abnormal uterine bleeding and changes in the uterine cavity seen by transvaginal ultrasonography were selected, and they were in

  20. Prevenção do Nascimento Prematuro: Importância da Monitorização das Contrações Uterinas Prevention of Preterm Birth: Role of Uterine Contraction Monitoring

    Directory of Open Access Journals (Sweden)

    Eduardo Sérgio Borges da Fonseca

    1999-10-01

    Full Text Available Objetivo: avaliar a relação entre alterações das contrações uterinas e parto prematuro. Metodologia: entre fevereiro de 1996 e junho de 1998, 73 gestantes com risco para o parto prematuro foram submetidas à monitorização externa das contrações uterinas, da 24ª à 34ª semana de gestação, duas vezes por semana, durante 60 minutos. O teste foi considerado positivo quando as contrações uterinas apresentavam freqüência maior ou igual a 4 contrações/hora antes da 30ª semana e a 6 contrações/hora após esta data. Resultado: foram excluídas da análise 17 pacientes (23,28% por apresentarem patologias obstétricas ou evolução desfavorável para os resultados finais. Das 56 gestantes restantes, a incidência de partos prematuros espontâneos foi de 23,21% (13/56. A freqüência média das contrações uterinas foi significativamente maior no grupo que evoluiu para o parto prematuro. O método revelou uma sensibilidade de 69,23%, uma especificidade de 86,04% e valores preditivos positivo e negativo de 60% e 90,24%, respectivamente. Conclusão: o teste negativo está associado a baixo risco de nascimento prematuro. Contudo, diante do teste positivo, torna-se necessária a associação com outros marcadores do parto prematuro para melhor identificar pacientes com risco elevado.Purpose: to evaluate the relationship between uterine contractions and premature delivery. Methods: between February 1996 and July 1998, 73 high risk pregnant women for preterm delivery, between the 24th and 34th weeks of gestation, were submitted to uterine contraction monitoring with tokodynamometers for 1 hour twice a week. The positive test was the presence of 4 contractions/h before the 30th week of gestation, and after this time, 6 contractions/h. Result: of 73 women, 17 patients (23.28% were excluded from the final analysis because they presented obstetric problems or unfavorable development for the final result. The rate of preterm delivery was 21

  1. Contractile 5-HT1B receptors in human cerebral arteries

    DEFF Research Database (Denmark)

    Nilsson, T; Longmore, J; Shaw, D;

    1999-01-01

    immunocytochemistry with antibodies selective for human 5-HT1B and human 5-HT1D receptors and also studied the contractile effects of a range of 5-HT receptor agonists and antagonists in HCA. 2 Immunocytochemistry of cerebral arteries showed dense 5-HT1B receptor immunoreactivity (but no 5-HT1D receptor......1 The cerebrovascular receptor(s) that mediates 5-hydroxytryptamine (5-HT)-induced vasoconstriction in human cerebral arteries (HCA)has proven difficult to characterize, yet these are essential in migraine. We have examined 5-HT receptor subtype distribution in cerebral blood vessels by...... immunoreactivity) within the smooth muscle wall of the HCA. The endothelial cell layer was well preserved and weak 5-HT1B receptor immunoreactivity was present. 3 Pharmacological experiments on HCA with intact endothelium showed that 5-carboxamidotryptamine was significantly more potent than alpha-methyl-5-HT, 2...

  2. Cardiac contractility, central haemodynamics and blood pressure regulation during semistarvation

    DEFF Research Database (Denmark)

    Stokholm, K H; Breum, L; Astrup, A

    1991-01-01

    pressure (BP) declined. The fall in BP was caused by the reduction in cardiac output as the total peripheral resistance was unchanged. Finally, the decline in total blood volume was not significant. These findings together with a reduction in heart rate indicated that a reduced sympathetic tone via......Eight obese patients were studied before and after 2 weeks of treatment by a very-low-calorie diet (VLCD). Cardiac output and central blood volume (pulmonary blood volume and left atrial volume) were determined by indicator dilution (125I-albumin) and radionuclide angiocardiography (first pass and...... equilibrium technique by [99Tcm]red blood cells). Cardiac output decreased concomitantly with the reduction in oxygen uptake as the calculated systemic arteriovenous difference of oxygen was unaltered. There were no significant decreases in left ventricular contractility indices, i.e. the ejection fraction...

  3. Effect of acute mesenteric ischemia on rat small intestinal contractility

    DEFF Research Database (Denmark)

    Zhao, Jingbo; Gregersen, Hans

    analyzed from the pressure-diameter curves. Distension-induced contraction thresholds and maximum contraction amplitude of basic and flow-induced contractions were calculated in terms of mechanical stress and strain. Differences among different groups were analyzed using t-test and ANOVA . Results: After...... ischemia for 60 and 120 min, the intestinal wall thickness and wall area were significantly smaller (p < 0.05); the maximum stresses of flow- induced contractions were lower at low outlet resistance pressures (p < 0.05); the maximum pressures and strain was lower at all outlet resistance pressures (p < 0.......05 and p < 0.01); the pressure and stress thresholds to induce phasic contraction were significantly higher during ramp distension (p < 0.05, p < 0.01). These abovementioned changes were not significant after ischemia for 15 and 30 min (p > 0.05). Conclusion: The intestinal contractility was significantly...

  4. Contractile reaction of isolated frog aorta after X-irradiation

    International Nuclear Information System (INIS)

    The action of X-rays (50 kV, filtered by 0.3 mm Al) on helical strip of frog aorta (rana esculenta) has been investigated. The isolated preparations have a stable basal tone and are radio-sensitive to X-rays which induce reversible, dose-dependent, contractile responses. After repeated irradiational tachyphylaxis appears. The threshold doses are about 250 R at 3 to 6 kR/min, antiadrenergic (phentolamine, propranolol), anticholinergic (atropin), antihistaminic (Neo-Bridal) and serotoninergic (Deseril) drugs have no visible influence on the X-ray induced reaction, i.e. these action mechanisms of the irradiation-induced contraction do not seem probable. Theophylline and cAMP inhibit the X-ray contraction probably non-specifically. Indometacin also inhibits the X-ray contraction: this suggests participation of prostaglandin-mechanism on the contraction of frog aorta after irradiation. (orig.)

  5. High-throughput screening for modulators of cellular contractile force

    CERN Document Server

    Park, Chan Young; Tambe, Dhananjay; Chen, Bohao; Lavoie, Tera; Dowell, Maria; Simeonov, Anton; Maloney, David J; Marinkovic, Aleksandar; Tschumperlin, Daniel J; Burger, Stephanie; Frykenberg, Matthew; Butler, James P; Stamer, W Daniel; Johnson, Mark; Solway, Julian; Fredberg, Jeffrey J; Krishnan, Ramaswamy

    2014-01-01

    When cellular contractile forces are central to pathophysiology, these forces comprise a logical target of therapy. Nevertheless, existing high-throughput screens are limited to upstream signaling intermediates with poorly defined relationship to such a physiological endpoint. Using cellular force as the target, here we screened libraries to identify novel drug candidates in the case of human airway smooth muscle cells in the context of asthma, and also in the case of Schlemm's canal endothelial cells in the context of glaucoma. This approach identified several drug candidates for both asthma and glaucoma. We attained rates of 1000 compounds per screening day, thus establishing a force-based cellular platform for high-throughput drug discovery.

  6. Atunero congelador al cerco de 1000 m3 de capacidad en cubas

    OpenAIRE

    Elena Vázquez, Adrián

    2015-01-01

    El proyecto que aquí se presenta consiste el diseño y calculo de un buque atunero congelador de pesca al cerco, el cual tiene como requerimientos de partida la siguiente especificación. Especificación Capacidad en cubas para el atún congelado 1000 m3 Velocidad en pruebas a 90% MCR 16 nudos Capacidad de combustible 500 m3 Capacidad de congelación 50 t/día Alojamiento 16 tripulantes Equipo propulsor Motor diesel con reductor Sociedad de clasificación American Bureau of S...

  7. Altas Capacidades en Educación Infantil en Guipúzcoa

    OpenAIRE

    Omaetxebarria-Aldarono, Ainara

    2013-01-01

    El trabajo fin de grado que se presenta a continuación aporta información acerca de las altas capacidades en Educación Infantil y su relación con las inteligencias múltiples. Se propone una perspectiva de la realidad actual del tema en el contexto de la provincia de Guipúzcoa. Las altas capacidades se proponen aludiendo a varios aspectos, entre ellos, se define el término, se aportan características de los alumnos con altas capacidades, se señalan alternativas de intervención en las aulas y s...

  8. La capacidad administrativa del gobierno del Distrito Federal y el cambio climático

    OpenAIRE

    Angélica Rosas Huerta

    2011-01-01

    En este momento existen varios gobiernos centrales y locales que están impulsando acciones orientadas a atender el cambio climático, sin embargo, los resultados pueden ser afectados, entre otros aspectos, por su propia capacidad institucional. En este sentido, el presente artículo busca analizar la capacidad institucional que el gobierno del Distrito Federal (DF) está construyendo para atender el cambio climático, a través de uno de sus componentes, la capacidad administrativa.

  9. Dynamic regulation of β1 subunit trafficking controls vascular contractility

    Science.gov (United States)

    Leo, M. Dennis; Bannister, John P.; Narayanan, Damodaran; Nair, Anitha; Grubbs, Jordan E.; Gabrick, Kyle S.; Boop, Frederick A.; Jaggar, Jonathan H.

    2014-01-01

    Ion channels composed of pore-forming and auxiliary subunits control physiological functions in virtually all cell types. A conventional view is that channels assemble with their auxiliary subunits before anterograde plasma membrane trafficking of the protein complex. Whether the multisubunit composition of surface channels is fixed following protein synthesis or flexible and open to acute and, potentially, rapid modulation to control activity and cellular excitability is unclear. Arterial smooth muscle cells (myocytes) express large-conductance Ca2+-activated potassium (BK) channel α and auxiliary β1 subunits that are functionally significant modulators of arterial contractility. Here, we show that native BKα subunits are primarily (∼95%) plasma membrane-localized in human and rat arterial myocytes. In contrast, only a small fraction (∼10%) of total β1 subunits are located at the cell surface. Immunofluorescence resonance energy transfer microscopy demonstrated that intracellular β1 subunits are stored within Rab11A-postive recycling endosomes. Nitric oxide (NO), acting via cGMP-dependent protein kinase, and cAMP-dependent pathways stimulated rapid (≤1 min) anterograde trafficking of β1 subunit-containing recycling endosomes, which increased surface β1 almost threefold. These β1 subunits associated with surface-resident BKα proteins, elevating channel Ca2+ sensitivity and activity. Our data also show that rapid β1 subunit anterograde trafficking is the primary mechanism by which NO activates myocyte BK channels and induces vasodilation. In summary, we show that rapid β1 subunit surface trafficking controls functional BK channel activity in arterial myocytes and vascular contractility. Conceivably, regulated auxiliary subunit trafficking may control ion channel activity in a wide variety of cell types. PMID:24464482

  10. Chronic Contractile Dysfunction without Hypertrophy Does Not Provoke a Compensatory Transcriptional Response in Mouse Hearts.

    Directory of Open Access Journals (Sweden)

    Scot J Matkovich

    Full Text Available Diseased myocardium from humans and experimental animal models shows heightened expression and activity of a specific subtype of phospholipase C (PLC, the splice variant PLCβ1b. Previous studies from our group showed that increasing PLCβ1b expression in adult mouse hearts by viral transduction was sufficient to cause sustained contractile dysfunction of rapid onset, which was maintained indefinitely in the absence of other pathological changes in the myocardium. We hypothesized that impaired contractility alone would be sufficient to induce a compensatory transcriptional response. Unbiased, comprehensive mRNA-sequencing was performed on 6 biological replicates of rAAV6-treated blank, PLCβ1b and PLCβ1a (closely related but inactive splice variant hearts 8 weeks after injection, when reduced contractility was manifest in PLCβ1b hearts without evidence of induced hypertrophy. Expression of PLCβ1b resulted in expression changes in only 9 genes at FDR<0.1 when compared with control and these genes appeared unrelated to contractility. Importantly, PLCβ1a caused similar mild expression changes to PLCβ1b, despite a complete lack of effect of this isoform on cardiac contractility. We conclude that contractile depression caused by PLCβ1b activation is largely independent of changes in the transcriptome, and thus that lowered contractility is not sufficient in itself to provoke measurable transcriptomic alterations. In addition, our data stress the importance of a stringent control group to filter out transcriptional changes unrelated to cardiac function.

  11. CAPACIDAD DE AGENCIA DE AUTOCUIDADO EN LAS PERSONAS ADULTAS QUE PADECEN ARTRITIS REUMATOIDE

    Directory of Open Access Journals (Sweden)

    Viriam Leiva Díaz

    2012-04-01

    Full Text Available El objetivo de la investigación consistió en analizar la capacidad de agencia de autocuidado que poseen laspersonas con diagnóstico de artritis reumatoide, quienes habitan en los cantones de Palmares y San Ramón. Se desarrolló una metodología de investigación cuantitativa de tipo descriptiva transversal. En cuanto a la población participante del estudio, estuvo conformada por 13 personas diagnosticadas con artritis reumatoide; respecto de los instrumentos utilizados para recolectar información, a los colaboradores se les aplicó el instrumento “Escala de apreciación de la capacidad de agencia de autocuidado”, propuesta por Dorothea Orem. Se determinó que los participantes poseen alta capacidad de agencia de autocuidado en las diferentes categorías, ya que únicamente se detectó baja capacidad de agencia de autocuidado en cuanto a interacción social (2 personas, y respecto del consumo insuficiente de alimentos (5 personas. La escala ASA permite a la Enfermera (o determinar la capacidad de agencia de autocuidado, de forma oportuna y eficaz. Además, permitió a las investigadoras observar un panorama general de las necesidades de autocuidado de la población participante y constatar que esta presenta alta capacidad de agencia de autocuidado.

  12. Qual o melhor período para a realização do doppler das artérias uterinas na predição de complicações da gestação? Which is the best period to perform uterine artery Doppler in the prediction of pregnancy complications?

    OpenAIRE

    Fabrício da Silva Costa; Sérgio Pereira da Cunha; Aderson Tadeu Berezowski

    2006-01-01

    OBJETIVO: Avaliar, prospectivamente, qual o melhor período para a realização do Doppler das artérias uterinas na predição de complicações da gestação. MATERIAIS E MÉTODOS: Foi conduzido estudo prospectivo em 45 pacientes primigestas, sem história de doenças crônicas. O Doppler das artérias uterinas foi realizado entre 18-20, 24-26, 28-30 e 34-36 semanas, com determinação do índice de resistência, índice de pulsatilidade, relação A/B e a presença ou ausência de incisura na onda de velocidade d...

  13. Quantifying esophagogastric junction contractility with a novel HRM topographic metric, the EGJ-Contractile Integral: normative values and preliminary evaluation in PPI non-responders

    Science.gov (United States)

    Nicodème, Frédéric; Pipa-Muniz, Maria; Khanna, Kern; Kahrilas, Peter J.; Pandolfino, John E.

    2015-01-01

    Background Despite its obvious pathophysiological relevance, the clinical utility of measures of esophagogastric junction (EGJ) contractility is unsubstantiated. High-resolution manometry (HRM) may improve upon this with its inherent ability to integrate the magnitude of contractility over time and length of the EGJ. This study aimed to develop a novel HRM metric summarizing EGJ contractility and test its ability distinguish among subgroups of proton pump inhibitor non-responders (PPI-NRs). Methods 75 normal controls and 88 PPI-NRs were studied. All underwent HRM. PPI-NRs underwent pH-impedance monitoring on PPI therapy scored in terms of acid exposure, number of reflux events, and reflux-symptom correlation and grouped as meeting All Criteria, Some Criteria, or No Criteria of abnormality. Control HRM studies were used to establish normal values for candidate EGJ contractility metrics, which were then compared in their ability to differentiate among PPI-NR subgroups. Results The EGJ contractile integral (EGJ-CI), a metric integrating contractility across the EGJ for three respiratory cycles, best distinguished the All Criteria PPI-NR subgroup from controls and other PPI-NR subgroups. Normal values (median, [IQR]) for this measure were 39 mmHg-cm [25–55 mmHg-cm]. The correlation between the EGJ-CI and a previously proposed metric, the lower esophageal sphincter-pressure integral, that used a fixed 10s time frame and an atmospheric as opposed to gastric pressure reference was weak. Conclusion Among HRM metrics tested, the EGJ-CI was best in distinguishing PPI-NRs meeting All Criteria of abnormality on pH-impedance testing. Future prospective studies are required to explore its utility in management of broader groups of GERD patients. PMID:24460814

  14. Nova pipeta para inseminação intra-uterina em suínos New pipette for intrauterine insemination in pigs

    Directory of Open Access Journals (Sweden)

    Gustavo Nogueira Diehl

    2006-02-01

    Full Text Available O objetivo deste trabalho foi avaliar o desempenho reprodutivo de 423 fêmeas suínas de ordem de parto 1 a 9 submetidas à inseminação intra-uterina (IAU, com um novo modelo de pipeta (T1 cuja extremidade não é fixada na cérvix ou uma pipeta de IAU modelo Verona® e que permite a fixação da sua extremidade em espiral na cérvix (T2. Para comparar as duas pipetas, foi considerado o grau de dificuldade para realização das inseminações, o tempo necessário para realizá-las, a presença de sangramento após a inseminação, a presença de refluxo no momento da inseminação, as taxas de retorno ao estro (TR, de prenhez (TPR e de parto ajustada (TPA, além do número de leitões nascidos (NT. As fêmeas de ambos os grupos foram inseminadas com doses de 1 bilhão de espermatozóides, em intervalos de 24 horas. A passagem do cateter de IAU através da cérvix foi possível em 95,9% das fêmeas, sem diferença entre os tratamentos (P>0,05. Em pelo menos uma das inseminações, foi observado sangue no cateter, após a realização da IAU, em 20,6% das fêmeas do T1 e 15,2% das fêmeas do T2 (P=0,14. O tempo médio necessário para realizar a inseminação foi de 2,1 minutos para o T1 e 2,3 minutos para o T2 (P=0,26. O percentual de fêmeas com refluxo de sêmen no momento da inseminação foi maior (P=0,01 no T1 (8,4% em comparação ao T2 (2,9%. Não houve diferença (P>0,05 nas variáveis TR (8,0 e 4,8%, TPR (93,4 e 96,2% e NT (12,4 e 12,7 leitões entre T1 e T2, respectivamente. A TPA do T1 (90,6% apresentou tendência (P=0,07 de ser inferior à do T2 (95,1%. No T1, as fêmeas primíparas apresentaram maior TR e menor TPA em comparação às pluríparas (PThe aim of this work was to evaluate the reproductive performance of 423 sows of 1 to 9 farrowings inseminated with two different intrauterine artificial insemination (IAU pipettes. The first group (T1 was formed by sows inseminated with a new pipette whose extremity was not fixed in the

  15. Malformação arteriovenosa uterina após doença trofoblástica gestacional Uterine arteriovenous malformation after gestational trophoblastic disease

    Directory of Open Access Journals (Sweden)

    Paulo Belfort

    2006-02-01

    Full Text Available OBJETIVO: investigar a presença e resultados de malformações vasculares uterinas (MAVU após doença trofoblástica gestacional (DTG. MÉTODOS: estudo retrospectivo com inclusão de casos diagnosticados entre 1987 e 2004; 2764 pacientes após DTG foram acompanhadas anualmente com ultra-sonografia transvaginal e Doppler colorido no Centro de Neoplasia Trofoblástica Gestacional da Santa Casa da Misericórdia (Rio de Janeiro, RJ, Brasil. Sete pacientes tiveram diagnóstico final de MAVU baseado em análise ultra-sonográfica - índice de pulsatilidade (IP, índice de resistência (IR e velocidade sistólica máxima (VSM - e achados de imagens de ressonância nuclear magnética (RNM. Dosagens negativas de beta-hCG foram decisivas para estabelecer o diagnóstico diferencial com DTG recidivante. RESULTADOS: a incidência de MAVU após DTG foi 0,2% (7/2764. Achados ultra-sonográficos de MAVU: IP médio de 0,44±0,058 (extremos: 0,38-0,52; IR médio de 0,36±0,072 (extremos: 0,29-0,50; VSM média de 64,6±23,99 cm/s (extremos: 37-96. A imagem de RNM revelou útero aumentado, miométrio heterogêneo, espaços vasculares tortuosos e vasos parametriais com ectasia. A apresentação clínica mais comum foi hemorragia transvaginal, presente em 52,7% (4/7 dos casos. Tratamento farmacológico com 150 mg de acetato de medroxiprogesterona foi empregado para controlar a hemorragia, após a estabilização hemodinâmica. Permanecem as pacientes em seguimento, assintomáticas até hoje. Duas pacientes engravidaram com MAVU, com gestações e partos exitosos. CONCLUSÃO: presente sangramento transvaginal em pacientes com beta-hCG negativo e história de DTG, deve-se considerar a possibilidade de MAVU e solicitar avaliação ultra-sonográfica com dopplervelocimetria. O tratamento conservador é a melhor opção na maioria dos casos de MAVU pós-DTG.PURPOSE: to investigate the presence and outcome of uterinevascular malformations (UVAM after gestational

  16. A comparative study of contractility of the heart ventricle in some ectothermic vertebrates

    OpenAIRE

    Sergey Kharin; Dmitry Shmakov

    2009-01-01

    The purpose of this study was to analyze contractility of the heart ventricle in selected reptilian and amphibian species having the same ventricular excitation pattern. Systolic time intervals and indices of contractility of the heart ventricle were measured in anaesthetized frogs, snakes, and tortoises by use of polycardiography. The electromechanical delay was significantly shorter in tortoises compared with the other two species. The isovolumetric contraction time in frogs was approximate...

  17. Remodelling of the contractile apparatus of striated muscle stimulated electrically in a shortened position.

    OpenAIRE

    Jakubiec-Puka, A; Carraro, U

    1991-01-01

    The aim of this study was to examine reorganisation of the contractile apparatus during adaptation to function when the length of a muscle is decreased. The rat soleus muscle was maintained in a shortened position and simultaneously stimulated electrically at a low frequency for 1-45 h. This experimental model decreased the length of the muscle and made the contractile apparatus irregular. The length of the sarcomeres decreased and became variable. The Z-line appeared wavy or fragmented. Foci...

  18. Motor-free actin bundle contractility driven by molecular crowding

    CERN Document Server

    Schnauß, Jörg; Schuldt, Carsten; Schmidt, B U Sebastian; Glaser, Martin; Strehle, Dan; Heussinger, Claus; Käs, Josef A

    2015-01-01

    Modeling approaches of suspended, rod-like particles and recent experimental data have shown that depletion forces display different signatures depending on the orientation of these particles. It has been shown that axial attraction of two rods yields contractile forces of 0.1pN that are independent of the relative axial shift of the two rods. Here, we measured depletion-caused interactions of actin bundles extending the phase space of single pairs of rods to a multi-particle system. In contrast to a filament pair, we found forces up to 3pN . Upon bundle relaxation forces decayed exponentially with a mean decay time of 3.4s . These different dynamics are explained within the frame of a mathematical model by taking pairwise interactions to a multi-filament scale. The macromolecular content employed for our experiments is well below the crowding of cells. Thus, we propose that arising forces can contribute to biological force generation without the need to convert chemical energy into mechanical work.

  19. Contractile Force of Human Extraocular Muscle: A Theoretical Analysis

    Science.gov (United States)

    Guo, Hongmei; Gao, Zhipeng; Chen, Weiyi

    2016-01-01

    Aim. The length-contractile force relationships of six human extraocular muscles (EOMs) in primary innervations should be determined during eye movement modeling and surgery of clinical EOMs. This study aims to investigate these relationships. Method. The proposal is based on the assumption that six EOMs have similar constitutive relationships, with the eye suspended in the primary position. The constitutive relationships of EOMs are obtained by optimizing from previous experimental data and the theory of mechanical equilibrium using traditional model. Further, simulate the existing experiment of resistance force, and then compare the simulated results with the existing experimental results. Finally, the mechanical constitutive relationships of EOMs are obtained. Results. The results show that the simulated resistance forces from the other four EOMs except for the horizontal recti well agree with previous experimental results. Conclusion. The mechanical constitutive relationships of six EOMs in primary innervations are obtained, and the rationality of the constitutive relationships is verified. Whereafter, the active stress-strain relationships of the six EOMs in the primary innervations are obtained. The research results can improve the eye movement model to predict the surgical amounts of EOMs before EOM surgery more precisely. PMID:27087774

  20. Considerations for Contractile Electroactive Polymeric Materials and Actuators

    Energy Technology Data Exchange (ETDEWEB)

    Rasmussen, Lenore; Erickson, Carl J.; Meixler, Lewis D.; Ascione, George; Gentile, Charles A.; Tilson, Charles; Bernasek, Stephen L.; Abelev, Esta

    2009-06-16

    Ras Labs produces electroactive polymer (EAP) based materials and actuators that bend, swell, ripple and now contract (new development) with low electric input. This is an important attribute because of the ability of contraction to produce life-like motion. The mechanism of contraction is not well understood. Radionuclide-labeled experiments were conducted to follow the movement of electrolytes and water in these EAPs when activated. Extreme temperature experiments were performed on the contractile EAPs with very favorable results. One of the biggest challenges in developing these actuators, however, is the electrode-EAP interface because of the pronounced movement of the EAP. Plasma treatments of metallic electrodes were investigated in order to improve the attachment of the embedded electrodes to the EAP material. Surface analysis, adhesive testing, and mechanical testing were conducted to test metal surfaces and metal-polymer interfaces. The nitrogen plasma treatment of titanium produced a strong metal-polymer interface; however, oxygen plasma treatment of both stainless steel and titanium produced even stronger metal-polymer interfaces. Plasma treatment of the electrodes allows for the embedded electrodes and the EAP material of the actuator to work and move as a unit, with no detachment, by significantly improving the metal-polymer interface.

  1. Contractile apparatus dysfunction early in thepathophysiology of diabetic cardiomyopathy

    Institute of Scientific and Technical Information of China (English)

    2015-01-01

    Diabetes mellitus significantly increases the risk ofcardiovascular disease and heart failure in patients.Independent of hypertension and coronary arterydisease, diabetes is associated with a specific cardiomyopathy,known as diabetic cardiomyopathy (DCM).Four decades of research in experimental animalmodels and advances in clinical imaging techniquessuggest that DCM is a progressive disease, beginningearly after the onset of type 1 and type 2 diabetes,ahead of left ventricular remodeling and overt diastolicdysfunction. Although the molecular pathogenesis ofearly DCM still remains largely unclear, activation ofprotein kinase C appears to be central in driving theoxidative stress dependent and independent pathwaysin the development of contractile dysfunction. Multiplesubcellular alterations to the cardiomyocyte are nowbeing highlighted as critical events in the early changesto the rate of force development, relaxation and stabilityunder pathophysiological stresses. These changes includeperturbed calcium handling, suppressed activity ofaerobic energy producing enzymes, altered transcriptionaland posttranslational modification of membrane andsarcomeric cytoskeletal proteins, reduced actin-myosincross-bridge cycling and dynamics, and changed myofilamentcalcium sensitivity. In this review, we will presentand discuss novel aspects of the molecular pathogenesisof early DCM, with a special focus on the sarcomericcontractile apparatus.

  2. Contractile Force of Human Extraocular Muscle: A Theoretical Analysis.

    Science.gov (United States)

    Guo, Hongmei; Gao, Zhipeng; Chen, Weiyi

    2016-01-01

    Aim. The length-contractile force relationships of six human extraocular muscles (EOMs) in primary innervations should be determined during eye movement modeling and surgery of clinical EOMs. This study aims to investigate these relationships. Method. The proposal is based on the assumption that six EOMs have similar constitutive relationships, with the eye suspended in the primary position. The constitutive relationships of EOMs are obtained by optimizing from previous experimental data and the theory of mechanical equilibrium using traditional model. Further, simulate the existing experiment of resistance force, and then compare the simulated results with the existing experimental results. Finally, the mechanical constitutive relationships of EOMs are obtained. Results. The results show that the simulated resistance forces from the other four EOMs except for the horizontal recti well agree with previous experimental results. Conclusion. The mechanical constitutive relationships of six EOMs in primary innervations are obtained, and the rationality of the constitutive relationships is verified. Whereafter, the active stress-strain relationships of the six EOMs in the primary innervations are obtained. The research results can improve the eye movement model to predict the surgical amounts of EOMs before EOM surgery more precisely. PMID:27087774

  3. Considerations for Contractile Electroactive Polymeric Materials and Actuators

    International Nuclear Information System (INIS)

    Ras Labs produces electroactive polymer (EAP) based materials and actuators that bend, swell, ripple and now contract (new development) with low electric input. This is an important attribute because of the ability of contraction to produce life-like motion. The mechanism of contraction is not well understood. Radionuclide-labeled experiments were conducted to follow the movement of electrolytes and water in these EAPs when activated. Extreme temperature experiments were performed on the contractile EAPs with very favorable results. One of the biggest challenges in developing these actuators, however, is the electrode-EAP interface because of the pronounced movement of the EAP. Plasma treatments of metallic electrodes were investigated in order to improve the attachment of the embedded electrodes to the EAP material. Surface analysis, adhesive testing, and mechanical testing were conducted to test metal surfaces and metal-polymer interfaces. The nitrogen plasma treatment of titanium produced a strong metal-polymer interface; however, oxygen plasma treatment of both stainless steel and titanium produced even stronger metal-polymer interfaces. Plasma treatment of the electrodes allows for the embedded electrodes and the EAP material of the actuator to work and move as a unit, with no detachment, by significantly improving the metal-polymer interface

  4. A comparative study of contractility of the heart ventricle in some ectothermic vertebrates

    Directory of Open Access Journals (Sweden)

    Sergey Kharin

    2009-07-01

    Full Text Available The purpose of this study was to analyze contractility of the heart ventricle in selected reptilian and amphibian species having the same ventricular excitation pattern. Systolic time intervals and indices of contractility of the heart ventricle were measured in anaesthetized frogs, snakes, and tortoises by use of polycardiography. The electromechanical delay was significantly shorter in tortoises compared with the other two species. The isovolumetric contraction time in frogs was approximately twofold longer than in reptiles. The pre-ejection period was the longest in frogs and the shortest in tortoises, whereas snakes were intermediate. The ejection time was slightly longer in tortoises compared with the other two species. The greatest isovolumetric contraction index and the smallest myocardial tension index corresponded to the frog and tortoise heart ventricle, respectively. The intrasystolic index in tortoises was significantly greater than in frogs, whereas quite similar to that in snakes. The frog ventricle had lower contractility compared with the reptilian one. Although ventricular contractility tended to be lower in snakes compared with tortoises, this difference was not statistically significant. Possible causes for these differences are discussed. We suppose a large variety in ventricular contractility among amphibian and reptilian species having the same ventricular activation pattern. This variety may be conditioned by heart anatomy, intracardiac shunting, lifestyles, and habitats. It can only be hypothesized that on the average, ventricular contractility is higher in reptiles compared with amphibians and in chelonians compared with snakes.

  5. Reliability of contractile properties of the knee extensor muscles in individuals with post-polio syndrome.

    Directory of Open Access Journals (Sweden)

    Eric L Voorn

    Full Text Available To assess the reliability of contractile properties of the knee extensor muscles in 23 individuals with post-polio syndrome (PPS and 18 age-matched healthy individuals.Contractile properties of the knee extensors were assessed from repeated electrically evoked contractions on 2 separate days, with the use of a fixed dynamometer. Reliability was determined for fatigue resistance, rate of torque development (MRTD, and early and late relaxation time (RT50 and RT25, using the intraclass correlation coefficient (ICC and standard error of measurement (SEM, expressed as % of the mean.In both groups, reliability for fatigue resistance was good, with high ICCs (>0.90 and small SEM values (PPS: 7.1%, healthy individuals: 7.0%. Reliability for contractile speed indices varied, with the best values found for RT50 (ICCs>0.82, SEM values <2.8%. We found no systematic differences between test and retest occasions, except for RT50 in healthy subjects (p = 0.016.In PPS and healthy individuals, the reliability of fatigue resistance, as obtained from electrically evoked contractions is high. The reliability of contractile speed is only moderate, except for RT50 in PPS, demonstrating high reliability.This was the first study to examine the reliability of electrically evoked contractile properties in individuals with PPS. Our results demonstrate its potential to study mechanisms underlying muscle fatigue in PPS and to evaluate changes in contractile properties over time in response to interventions or from natural course.

  6. Chronic Contractile Dysfunction without Hypertrophy Does Not Provoke a Compensatory Transcriptional Response in Mouse Hearts.

    Science.gov (United States)

    Matkovich, Scot J; Grubb, David R; McMullen, Julie R; Woodcock, Elizabeth A

    2016-01-01

    Diseased myocardium from humans and experimental animal models shows heightened expression and activity of a specific subtype of phospholipase C (PLC), the splice variant PLCβ1b. Previous studies from our group showed that increasing PLCβ1b expression in adult mouse hearts by viral transduction was sufficient to cause sustained contractile dysfunction of rapid onset, which was maintained indefinitely in the absence of other pathological changes in the myocardium. We hypothesized that impaired contractility alone would be sufficient to induce a compensatory transcriptional response. Unbiased, comprehensive mRNA-sequencing was performed on 6 biological replicates of rAAV6-treated blank, PLCβ1b and PLCβ1a (closely related but inactive splice variant) hearts 8 weeks after injection, when reduced contractility was manifest in PLCβ1b hearts without evidence of induced hypertrophy. Expression of PLCβ1b resulted in expression changes in only 9 genes at FDRcaused similar mild expression changes to PLCβ1b, despite a complete lack of effect of this isoform on cardiac contractility. We conclude that contractile depression caused by PLCβ1b activation is largely independent of changes in the transcriptome, and thus that lowered contractility is not sufficient in itself to provoke measurable transcriptomic alterations. In addition, our data stress the importance of a stringent control group to filter out transcriptional changes unrelated to cardiac function. PMID:27359099

  7. Menthol inhibits detrusor contractility independently of TRPM8 activation.

    Directory of Open Access Journals (Sweden)

    Antonio Celso Saragossa Ramos-Filho

    Full Text Available Agonists such as icilin and menthol can activate the cool temperature-sensitive ion channel TRPM8. However, biological responses to menthol may occur independently of TRPM8 activation. In the rodent urinary bladder, menthol facilitates the micturition reflex but inhibits muscarinic contractions of the detrusor smooth muscle. The site(s of TRPM8 expression in the bladder are controversial. In this study we investigated the regulation of bladder contractility in vitro by menthol. Bladder strips from wild type and TRPM8 knockout male mice (25-30 g were dissected free and mounted in organ baths. Isometric contractions to carbachol (1 nM-30 µM, CaCl2 (1 µM to 100 mM and electrical field stimulation (EFS; 8, 16, 32 Hz were measured. Strips from both groups contracted similarly in response to both carbachol and EFS. Menthol (300 µM or nifedipine (1 µM inhibited carbachol and EFS-induced contractions in both wild type and TRPM8 knockout bladder strips. Incubation with the sodium channel blocker tetrodotoxin (1 µM, replacement of extracellular sodium with the impermeant cation N-Methyl-D-Glucamine, incubation with a cocktail of potassium channel inhibitors (100 nM charybdotoxin, 1 µM apamin, 10 µM glibenclamide and 1 µM tetraethylammonium or removal of the urothelium did not affect the inhibitory actions of menthol. Contraction to CaCl2 was markedly inhibited by either menthol or nifedipine. In cultured bladder smooth muscle cells, menthol or nifedipine abrogated the carbachol or KCl-induced increases in [Ca2+]i. Intravesical administration of menthol increased voiding frequency while decreasing peak voiding pressure. We conclude that menthol inhibits muscarinic bladder contractions through blockade of L-type calcium channels, independently of TRPM8 activation.

  8. Procesos de automatización cognitiva en alumnado con altas capacidades intelectuales

    Directory of Open Access Journals (Sweden)

    Juan Montero-Linares

    2013-05-01

    Full Text Available Proponemos desde el enfoque del procesamiento de la información, el estudio de características cognitivas que faciliten la identificación de las altas capacidades. A tal fin, elaboramos una tarea de habilidad de segmentación silábica que, a partir del modelo propuesto por Baddeley sobre la memoria de trabajo y la distinción entre procesos automáticos y controlados, nos permitiese comprobar las diferencias en cuanto a la capacidad de automatización de la información y si los indicadores propuestos en la misma, tenían suficiente poder clasificatorio o predictivo en la identificación de la alta capacidad intelectual. Participaron 480 niños y niñas de primero a cuarto de Educación Primaria. Se clasificaron, en función de su CI, en dos grupos: alumnado de altas capacidades y alumnado de capacidades medias. Se les administró una batería de pruebas entre las que se encontraba el test de habilidad de segmentación silábica, lo que permitió realizar su estandarización. Los resultados mostraron que los alumnos con CI superior a 130 fueron capaces de automatizar la segmentación de forma más eficiente que los de inteligencia media. La medición de los procesos automáticos a través del test de habilidad de segmentación silábica, permitió diferenciar a los participantes de altas capacidades de los que no lo eran.

  9. Capacidad de adsorción de sulfato de un suelo forestal mediterráneo.

    OpenAIRE

    Quilchano, Consuelo; Gallardo, Juan F.

    2001-01-01

    En el presente estudio se ha evaluado la capacidad de adsorción de sulfato en un suelo forestal con vegetación mediterránea. El objetivo del trabajo es conocer la capacidad de adsorción de sulfato en cada uno de los horizontes del suelo, ya que se trata de un proceso responsable de la retención de este nutriente esencial (S) en el ecosistema, impidiendo su pérdida por lavado. La lixiviación de sulfato, además de reducir la fertilidad del suelo y generar problemas de contaminación en los ecosi...

  10. ALTAS CAPACIDADES: COMPARATIVA ENTRE EL MODELO ESPAÑOL Y BRITÁNICO

    OpenAIRE

    Rodríguez-Vázquez, María

    2012-01-01

    El presente trabajo de investigación exploratoria reflexiona sobre el concepto de alumno con altas capacidades y propone una comparación legislativa entre el modelo de atención a los alumnos con altas capacidades británico y español y cómo ese modelo afecta al sistema educativo general, ya que aunque tienen puntos en común entre ambos países, pero se trata dos formas de enfocar completamente distintas. Además también pretende recoger información sobre iniciativas y proyectos que reflejen l...

  11. Altas capacidades: Proyecto educativo para el aula de Educación Primaria

    OpenAIRE

    López-Presedo, Iria Flavia

    2015-01-01

    El presente TFG tiene como principal finalidad dar una respuesta educativa, eminentemente práctica, al alumnado de altas capacidades dentro del grupo-clase. En primer lugar, se han revisado las teorías de varios autores sobre las altas capacidades, los métodos de detección e intervención con este tipo de alumnado y, finalmente, se ha elaborado un proyecto en forma de programa de enriquecimiento curricular. Este proyecto se inscribe dentro de la Teoría de las inteligencias múltiples de Gard...

  12. Altas capacidades intelectuales : investigación y propuesta desde las inteligencias múltiples

    OpenAIRE

    Pulido Vinuesa, Salomé

    2015-01-01

    El presente trabajo trata sobre las altas capacidades, investigando sobre ello acerca de su historia y evolución, los diversos tipos existentes, sus características, su identificación, su evaluación, sus metodologías, entre otros. Además, se ha realizado un resumen de cómo es tratada esta necesidad educativa por las leyes, antiguas y en vigor. De igual modo, se trata la educación inclusiva, ya que es un tema que afecta a alumnos/as con altas capacidades. El programa de intervención en rel...

  13. Procesos de automatización cognitiva en alumnado con altas capacidades intelectuales

    OpenAIRE

    Juan Montero-Linares; José I. Navarro-Guzmán; Manuel Aguilar-Villagrán

    2013-01-01

    Proponemos desde el enfoque del procesamiento de la información, el estudio de características cognitivas que faciliten la identificación de las altas capacidades. A tal fin, elaboramos una tarea de habilidad de segmentación silábica que, a partir del modelo propuesto por Baddeley sobre la memoria de trabajo y la distinción entre procesos automáticos y controlados, nos permitiese comprobar las diferencias en cuanto a la capacidad de automatización de la información y si los indicadores propue...

  14. El alumnado con altas capacidades intelectuales: programas de intervención.

    OpenAIRE

    González Arranz, Raquel

    2015-01-01

    El presente Trabajo de Fin de Grado se centra en alumnos considerados de Educación Especial, concretamente en aquellos que poseen Altas Capacidades Intelectuales y las posibilidades de respuesta educativa que se les puede ofrecer dentro del modelo de aula ordinaria. En la primera parte, se afronta el estudio de alumnos con altas capacidades intelectuales en educación primaria. Los temas que se tratan son: conceptualización y definiciones, fundamentación teórica de alumnos con altas capaci...

  15. La autopercepción de las capacidades : origen social y elecciones educativas

    OpenAIRE

    Torrents Vilà, Dani

    2016-01-01

    Este texto analiza el efecto del origen social de los estudiantes en la autopercepción de sus capacidades, una vez controladas las capacidades objetivas. La autopercepción está evaluada en dos dimensiones: la autoubicación especular y las expectativas de resultados. Se trata de una muestra de 1782 estudiantes de 4º de ESO, derivada de ISCY Project . Mediante el análisis bivariado y trivariado de tablas de contingencia y comparación de proporciones, se ha observado un ligero sobreoptimismo de ...

  16. Las capacidades tecnológicas para la innovación en empresas de manufactura

    OpenAIRE

    Arturo García Velázquez; Daniel Pineda Domínguez; María Antonieta Andrade Vallejo

    2016-01-01

    Las capacidades tecnológicas son habilidades requeridas para un uso efectivo del conocimiento tecnológico. Permiten que las empresas de manufactura puedan innovar, un requisito básico para competir de manera efectiva en los mercados. El objetivo de este trabajo de investigación fue analizar los factores que relacionan las capacidades tecnológicas con la innovación. Para ello se hizo un análisis de la literatura, considerando los aportes de múltiples autores relevantes. Se contrastaron, además...

  17. Relação familiar da pessoa idosa com comprometimento da capacidade funcional

    OpenAIRE

    Luana Araújo dos Reis; Nadirlene Pereira Gomes; Luciana Araújo dos Reis; Tânia Maria de Oliva Menezes; Telmara Menezes Couto; Aline Cristiane de Souza Azevedo Aguiar; Margarida da Silva Neves de Abreu

    2015-01-01

    Objetivo: comprender la relación familiar de la persona adulta con comprometimiento de la capacidad funcional. Materiales y mé - todos: estudio exploratorio y descriptivo de abordaje cualitativo, fundamentado en el método de la historia oral, realizado con 15 adultos mayores asistidos por una unidad de salud de la familia, residentes con familiares y que presentaban comprometimiento de la capacidad funcional. Se recolectaron los datos en el período de marzo y abril del 2012, por medio de ...

  18. Capacidad funcional y salud: orientaciones para cuidar al adulto mayor1

    OpenAIRE

    GIRALDO M, CLARA INÉS

    2010-01-01

    Objetivo: valorar la salud y la capacidad funcional de ancianos con dependencia funcional para el autocuidado, como elementos para orientar el cuidado de enfermería y el cuidado familiar en casa. Metodología: estudio descriptivo transversal en 40 personas de 65 y más años con dependencia funcional, cuidados por familiar, en Envigado Colombia; muestreo por conveniencia. Valoración de la capacidad funcional con el índice de Katz y la escala de Lawton y Brody, ajustados según grupo de Neuro...

  19. THE IMPROVEMENT OF INFARCTED MYOCARDIAL CONTRACTILE FORCE AFTER AUTOLOGOUS SKELETAL MUSCLE SATELLITE CELL IMPLANTATION

    Institute of Scientific and Technical Information of China (English)

    钟竑; 朱洪生; 张臻

    2002-01-01

    Objective To study the improvement of infarcted myocardial contractile force after autologous skeletal muscle satellite cell implantation via intracoronary arterial perfusion. Methods Skeletal muscle cells were harvested from gluteus max of adult mongrel dogs and the cells were cultured and expanded before being labeled with DAPI (4, 6-diamidino-2-phenylindone). The labeled cells were then implanted into the acute myocardial infarct site via the ligated left anterior descending (LAD) coronary artery. Specimens were taken at 2nd, 4th, 8th week after myoblast implantation for histologic and contractile force evaluation, respectively. Results The satellite cells with fluorescence had been observed in the infarct site and also in papi-llary muscle with consistent oriented direction of host myocardium. A portion of the implanted cells had differen-tiated into muscle fibers. Two weeks after implantation, the myocardial contractile force showed no significant difference between the cell implant group and control group. At 4 and 8 week, the contractile force in the cell implant group was better than that in control group. Conclusion The skeletal muscle satellite cells, implanted into infarct myocardium by intracoronary arterial perfusion, could disseminate through the entire infarcted zone with myocardial regeneration and improve the contractile function of the infarcted myocardium.

  20. Temporal Adaptive Changes in Contractility and Fatigability of Diaphragm Muscles from Streptozotocin-Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Marco Brotto

    2010-01-01

    Full Text Available Diabetes is characterized by ventilatory depression due to decreased diaphragm (DPH function. This study investigated the changes in contractile properties of rat DPH muscles over a time interval encompassing from 4 days to 14 weeks after the onset of streptozotocin-induced diabetes, with and without insulin treatment for 2 weeks. Maximum tetanic force in intact DPH muscle strips and recovery from fatiguing stimulation were measured. An early (4-day depression in contractile function in diabetic DPH was followed by gradual improvement in muscle function and fatigue recovery (8 weeks. DPH contractile function deteriorated again at 14 weeks, a process that was completely reversed by insulin treatment. Maximal contractile force and calcium sensitivity assessed in Triton-skinned DPH fibers showed a similar bimodal pattern and the same beneficial effect of insulin treatment. While an extensive analysis of the isoforms of the contractile and regulatory proteins was not conducted, Western blot analysis of tropomyosin suggests that the changes in diabetic DPH response depended, at least in part, on a switch in fiber type.

  1. EFFECTS OF DESENSITIZATION AND REBOUND TO ADENOSINE ON ACTION POTENTIAL AND CONTRACTILITY IN ATRIAL CELLS IN GUINEA-PIGS

    Institute of Scientific and Technical Information of China (English)

    张凤杰; 臧伟进; 于晓江; 胡浩; 张春虹; 孙强; 吕军

    2002-01-01

    Objective To investigate the effects of desensitization and rebound to adenosine(Ado) on action potential duration(APD) and contractility in guinea-pig atrial cells. Methods Electrical activity was recorded using standard intracellular microelectrode technique and contractility was recorded using. We studied the effects of adenosine on the action potential and desensitization of contractility and rebound of contractility. Results The results showed that action potential duration were shortened by 1,10,100μmol*L-1Ado, the ratio of shortened APD was (9.58±1.40)%,(13.80±2.26)%,(24.80±3.19)%, respectively. 1μmol*L-1Ado had no desensitization (P>0.05), but the time of desensitization of 10μmol*L-1 Ado and 100μmol*L-1 Ado was 1 minute(P<0.05) and 5 minutes(P<0.05), respectively. The desensitization of contractility of 10*!μmol*L-1 Ado was obvious in atrial cells, the decrease of contractility of 10*!μmol*L-1 Ado was obvious in atrial cells, the decrease of contractility was changed from (31.4±16.04)%(2 minutes) to (50.60±15.87)% (4 minutes), compared with control. After washing out Ado, contractility was shown to rebound, the ratio of increase of contractility by 1,10,100μmol*L-1 Ado was (12.38±7.50)%,(19.00±8.14)% and (27.60±13.44)%, respectively. Conclusion Ado can abbreviate APD in atrial cells. The desensitization of Ado on APD is characterized by concentration-dependent and time-dependent in atrial cells, and the desensitization of contractility of Ado is obvious and contractility was shown to rebound after washing out Ado.

  2. Select forelimb muscles have evolved superfast contractile speed to support acrobatic social displays.

    Science.gov (United States)

    Fuxjager, Matthew J; Goller, Franz; Dirkse, Annika; Sanin, Gloria D; Garcia, Sarah

    2016-01-01

    Many species perform rapid limb movements as part of their elaborate courtship displays. However, because muscle performance is constrained by trade-offs between contraction speed and force, it is unclear how animals evolve the ability to produce both unusually fast appendage movement and limb force needed for locomotion. To address this issue, we compare the twitch speeds of forelimb muscles in a group of volant passerine birds, which produce different courtship displays. Our results show that the two taxa that perform exceptionally fast wing displays have evolved 'superfast' contractile kinetics in their main humeral retractor muscle. By contrast, the two muscles that generate the majority of aerodynamic force for flight show unmodified contractile kinetics. Altogether, these results suggest that muscle-specific adaptations in contractile speed allow certain birds to circumvent the intrinsic trade-off between muscular speed and force, and thereby use their forelimbs for both rapid gestural displays and powered locomotion. PMID:27067379

  3. Mechanobiological induction of long-range contractility by diffusing biomolecules and size scaling in cell assemblies

    Science.gov (United States)

    Dasbiswas, K.; Alster, E.; Safran, S. A.

    2016-01-01

    Mechanobiological studies of cell assemblies have generally focused on cells that are, in principle, identical. Here we predict theoretically the effect on cells in culture of locally introduced biochemical signals that diffuse and locally induce cytoskeletal contractility which is initially small. In steady-state, both the concentration profile of the signaling molecule as well as the contractility profile of the cell assembly are inhomogeneous, with a characteristic length that can be of the order of the system size. The long-range nature of this state originates in the elastic interactions of contractile cells (similar to long-range “macroscopic modes” in non-living elastic inclusions) and the non-linear diffusion of the signaling molecules, here termed mechanogens. We suggest model experiments on cell assemblies on substrates that can test the theory as a prelude to its applicability in embryo development where spatial gradients of morphogens initiate cellular development. PMID:27283037

  4. Mechanobiological induction of long-range contractility by diffusing biomolecules and size scaling in cell assemblies.

    Science.gov (United States)

    Dasbiswas, K; Alster, E; Safran, S A

    2016-01-01

    Mechanobiological studies of cell assemblies have generally focused on cells that are, in principle, identical. Here we predict theoretically the effect on cells in culture of locally introduced biochemical signals that diffuse and locally induce cytoskeletal contractility which is initially small. In steady-state, both the concentration profile of the signaling molecule as well as the contractility profile of the cell assembly are inhomogeneous, with a characteristic length that can be of the order of the system size. The long-range nature of this state originates in the elastic interactions of contractile cells (similar to long-range "macroscopic modes" in non-living elastic inclusions) and the non-linear diffusion of the signaling molecules, here termed mechanogens. We suggest model experiments on cell assemblies on substrates that can test the theory as a prelude to its applicability in embryo development where spatial gradients of morphogens initiate cellular development. PMID:27283037

  5. Mechanobiological induction of long-range contractility by diffusing biomolecules and size scaling in cell assemblies

    Science.gov (United States)

    Dasbiswas, K.; Alster, E.; Safran, S. A.

    2016-06-01

    Mechanobiological studies of cell assemblies have generally focused on cells that are, in principle, identical. Here we predict theoretically the effect on cells in culture of locally introduced biochemical signals that diffuse and locally induce cytoskeletal contractility which is initially small. In steady-state, both the concentration profile of the signaling molecule as well as the contractility profile of the cell assembly are inhomogeneous, with a characteristic length that can be of the order of the system size. The long-range nature of this state originates in the elastic interactions of contractile cells (similar to long-range “macroscopic modes” in non-living elastic inclusions) and the non-linear diffusion of the signaling molecules, here termed mechanogens. We suggest model experiments on cell assemblies on substrates that can test the theory as a prelude to its applicability in embryo development where spatial gradients of morphogens initiate cellular development.

  6. La evaluación psicopedagógica del alumnado con altas capacidades

    OpenAIRE

    Grau Rubio, Claudia

    2012-01-01

    Se describen los instrumentos de evaluación psicopedagógica aplicables al alumnado con altas capacidades. Instrumentos referidos al alumno, al contexto escolar, a las variables socio-familiares y la determinación de las necesidades educativas especiales.

  7. Capacidad antioxidante y contenido de polifenoles de 21 extractos de plantas medicinales argentinas

    OpenAIRE

    Tournier, H.; Fioravanti, D.; Dade, Martín; Schinella, Guillermo

    2010-01-01

    En este trabajo se evaluó la capacidad antioxidante total (CAT) y el contenido de fenoles totales (FT) y flavonoides (FV) de 21 extractos acuosos obtenidos de plantas nativas del valle de Traslasierra, Córdoba que son ampliamente utilizadas por poblaciones rurales y urbanas de nuestro país.

  8. Actions of genistein on contractile response of smooth muscle isolated from guinea pig gallbladder

    Institute of Scientific and Technical Information of China (English)

    Ya-Li Luo; Ya-Li Wang; Neng-Lian Li; Tian-Zhen Zheng; Li Zhang; Ya-Li She; Shu-Ming Hu

    2009-01-01

    BACKGROUND: Defective contractile motility of the gallbladder is an important factor for gallstone formation. Estrogen might increase the risk of gallstones and cholecystitis, and estradiol inhibits the contractile activity of isolated strips of guinea pig gallbladder. The potential risks associated with hormone replacement therapy (HRT) include symptomatic gallstones. Phytoestrogen have been used to treat menopause syndromes by replacing traditional estrogen. This experiment aimed to determine the effects of the phytoestrogen genistein on the contractile response of smooth muscle strips isolated from guinea pig gallbladder and its possible mechanism of action. METHODS: Guinea pigs were sacriifced to remove the whole gallbladder. Two or three smooth muscle strips were cut longitudinally. Each strip was suspended in a tissue chamber containing Krebs solution. After 2 hours of equilibration, contractile response indexes were recorded. Different concentrations of genistein were added to the chamber and the contractile responses were measured. Each antagonist was added 2 minutes before genistein to study possible mechanisms. The effect of genistein on calcium-dependent contraction curves and biphasic contraction in calcium-free Krebs solution were measured. RESULTS: Genistein decreased the resting tension dose-dependently, and reduced the mean contractile amplitude and frequency in gallbladder strips. Ranitidine partly inhibited the effect of genistein, but methylene blue, Nω-nitro-L-arginine, and propranolol hydrochloride did not inlfuence this action. Genistein had no signiifcant effects on calcium-dependent contraction. Genistein reduced the ifrst contraction induced by acetylcholine chloride, but did not affect the second contraction caused by CaCl2. CONCLUSIONS: Genistein relaxed smooth muscle isolated from the gallbladder of guinea pigs and this might contribute to the formation of gallstones. The inhibitory action might be related to H2 receptors and

  9. The Effects of Histamine H3 Receptors on Contractile Responses on Rat Gastric Fundus

    Directory of Open Access Journals (Sweden)

    Aşkın Hekimoğlu

    2006-01-01

    Full Text Available The aim of this study is to determine the effects of histamine receptors on the gastrointestinal system smooth muscle contractions and the role of histamine H3 receptors on these effects. İsolated rat gastric fundus preparations were hanged on isolated organ bath and histamine receptor agonist and anthagonists were added to the bath solution and the electrical field stimulation-induced contractile responses were evaluated. In our study groups after blocking one of the histamine receptors H1, H2,H3; contractile responses were observed. Then, other two receptors were blocked one by one or combination of them to observe the changes on the contractile responses given to the electrical stimulation .To blocke histamine receptors pyrilamine (10-6м as H1 receptor blocker, famotidine (10-6м as H2 receptor blocker and thioperamide (10-5м as H3 receptor blocker and various combination of them were used. All groups were treated with H3 receptor anthagonist thioperamide (10-5м and agonist (R-α-methylhistamine (RMHA on 10-8, 10-7, 10-6 ve 10-5 molar concentrations cumulatively to observe its mediator effects on contractile responses. We suggested that (R-α-methylhistamine mediates the inhibition on the contractile effects of rat gastric fundus. This conclusion was supported by these findings: a the selective agonists (RMHA caused a dumping of the contractile effect of acetylcholine; b the effect of (RMHA was prevented by the selective H3 receptor antagonist thioperamide.

  10. Accessory left atrial diverticulae: contractile properties depicted with 64-slice cine-cardiac CT.

    LENUS (Irish Health Repository)

    Killeen, Ronan P

    2012-02-01

    To assess the contractility of accessory left atrial appendages (LAAs) using multiphasic cardiac CT. We retrospectively analyzed the presence, location, size and contractile properties of accessory LAAs using multiphasic cardiac 64-slice CT in 102 consecutive patients (63 males, 39 females, mean age 57). Multiplanar reformats were used to create image planes in axial oblique, sagittal oblique and coronal oblique planes. For all appendages with an orifice diameter >or= 10 mm, axial and sagittal diameters and appendage volumes were recorded in atrial diastole and systole. Regression analysis was performed to assess which imaging appearances best predicted accessory appendage contractility. Twenty-three (23%) patients demonstrated an accessory LAA, all identified along the anterior LA wall. Dimensions for axial oblique (AOD) and sagittal oblique (SOD) diameters and sagittal oblique length (SOL) were 6.3-19, 3.4-20 and 5-21 mm, respectively. All appendages (>or=10 mm) demonstrated significant contraction during atrial systole (greatest diameter reduction was AOD [3.8 mm, 27%]). Significant correlations were noted between AOD-contraction and AOD (R = 0.57, P < 0.05) and SOD-contraction and AOD, SOD and SOL (R = 0.6, P < 0.05). Mean diverticulum volume in atrial diastole was 468.4 +\\/- 493 mm(3) and in systole was 171.2 +\\/- 122 mm(3), indicating a mean change in volume of 297.2 +\\/- 390 mm(3), P < 0.0001. Stepwise multiple regression analysis revealed SOL to be the strongest independent predictor of appendage contractility (R(2) = 0.86, P < 0.0001) followed by SOD (R(2) = 0.91, P < 0.0001). Accessory LAAs show significant contractile properties on cardiac CT. Those accessory LAAs with a large sagittal height or depth should be evaluated for contractile properties, and if present should be examined for ectopic activity during electrophysiological studies.

  11. Effect of a crude sulfated polysaccharide from Halymenia floresia (Rhodophyta) on gastrointestinal smooth muscle contractility

    OpenAIRE

    José Ronaldo Vasconcelos Graça; Mirna Marques Bezerra; Vilma Lima; José Ariévilo Gurgel Rodrigues; Diego Levi Silveira Monteiro; Ana Luíza Gomes Quinderé; Rodrigo César das Neves Amorim; Regina Célia Monteiro de Paula; Norma Maria Barros Benevides

    2011-01-01

    The aim of this work was to study the effect of Halymenia floresia (Hf) on duodenum contractility, and on experimental protocols of gastric compliance (GC) in rats. Fraction Hf2s exhibited a concentration-dependent myocontractile effect (EC50 12.48 µg/ml), and an inhibitory effect after consecutive washing. The contractile response promoted by Hf2s in the duodenum strips was completely inhibited by verapamil, and the effects were prevented in the presence of Ca2+-free medium. The pretreatment...

  12. Validation of an in vitro contractility assay using canine ventricular myocytes

    International Nuclear Information System (INIS)

    Measurement of cardiac contractility is a logical part of pre-clinical safety assessment in a drug discovery project, particularly if a risk has been identified or is suspected based on the primary- or non-target pharmacology. However, there are limited validated assays available that can be used to screen several compounds in order to identify and eliminate inotropic liability from a chemical series. We have therefore sought to develop an in vitro model with sufficient throughput for this purpose. Dog ventricular myocytes were isolated using a collagenase perfusion technique and placed in a perfused recording chamber on the stage of a microscope at ∼ 36 °C. Myocytes were stimulated to contract at a pacing frequency of 1 Hz and a digital, cell geometry measurement system (IonOptix™) was used to measure sarcomere shortening in single myocytes. After perfusion with vehicle (0.1% DMSO), concentration–effect curves were constructed for each compound in 4–30 myocytes taken from 1 or 2 dog hearts. The validation test-set was 22 negative and 8 positive inotropes, and 21 inactive compounds, as defined by their effect in dog, cynolomolgous monkey or humans. By comparing the outcome of the assay to the known in vivo contractility effects, the assay sensitivity was 81%, specificity was 75%, and accuracy was 78%. With a throughput of 6–8 compounds/week from 1 cell isolation, this assay may be of value to drug discovery projects to screen for direct contractility effects and, if a hazard is identified, help identify inactive compounds. -- Highlights: ► Cardiac contractility is an important physiological function of the heart. ► Assessment of contractility is a logical part of pre-clinical drug safety testing. ► There are limited validated assays that predict effects of compounds on contractility. ► Using dog myocytes, we have developed an in vitro cardiac contractility assay. ► The assay predicted the in vivo contractility with a good level of accuracy.

  13. Validation of an in vitro contractility assay using canine ventricular myocytes

    Energy Technology Data Exchange (ETDEWEB)

    Harmer, A.R., E-mail: alex.harmer@astrazeneca.com; Abi-Gerges, N.; Morton, M.J.; Pullen, G.F.; Valentin, J.P.; Pollard, C.E.

    2012-04-15

    Measurement of cardiac contractility is a logical part of pre-clinical safety assessment in a drug discovery project, particularly if a risk has been identified or is suspected based on the primary- or non-target pharmacology. However, there are limited validated assays available that can be used to screen several compounds in order to identify and eliminate inotropic liability from a chemical series. We have therefore sought to develop an in vitro model with sufficient throughput for this purpose. Dog ventricular myocytes were isolated using a collagenase perfusion technique and placed in a perfused recording chamber on the stage of a microscope at ∼ 36 °C. Myocytes were stimulated to contract at a pacing frequency of 1 Hz and a digital, cell geometry measurement system (IonOptix™) was used to measure sarcomere shortening in single myocytes. After perfusion with vehicle (0.1% DMSO), concentration–effect curves were constructed for each compound in 4–30 myocytes taken from 1 or 2 dog hearts. The validation test-set was 22 negative and 8 positive inotropes, and 21 inactive compounds, as defined by their effect in dog, cynolomolgous monkey or humans. By comparing the outcome of the assay to the known in vivo contractility effects, the assay sensitivity was 81%, specificity was 75%, and accuracy was 78%. With a throughput of 6–8 compounds/week from 1 cell isolation, this assay may be of value to drug discovery projects to screen for direct contractility effects and, if a hazard is identified, help identify inactive compounds. -- Highlights: ► Cardiac contractility is an important physiological function of the heart. ► Assessment of contractility is a logical part of pre-clinical drug safety testing. ► There are limited validated assays that predict effects of compounds on contractility. ► Using dog myocytes, we have developed an in vitro cardiac contractility assay. ► The assay predicted the in vivo contractility with a good level of accuracy.

  14. Inhibition by sildenafil of contractility of isolated non-pregnant human myometrium

    OpenAIRE

    Aruldhas Blessed Winston; Kaysina Vazhudhi; Sumalya Sen; Elsy Thomas; Santhosh Benjamin; Jacob Peedicayil

    2015-01-01

    Objective: To investigate the ability of sildenafil to inhibit the contractility of isolated non pregnant human myometrium. Materials and Methods: The inhibitory effect of three concentrations (3, 10, and 30 µM) of sildenafil on 55 mM KCl-induced contractility of isolated non-pregnant human myometrium was studied. The ability of the guanylyl cyclase inhibitor ODQ (10 µM), the adenylyl cyclase inhibitor MDL-12,330A (10 µM), the non-specific potassium channel blocker TEA (2 mM), and the calcium...

  15. TEOR DE FENÓLICOS TOTAIS E CAPACIDADE ANTIOXIDANTE DE POLPAS CONGELADAS DE FRUTAS

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    E. A. MELO

    2008-12-01

    Full Text Available

    Polpas congeladas de frutas comercializadas na Cidade do Recife-PE foram submetidas à determinação do teor de fenólicos totais e da capacidade de seqüestrar o radical 1,1-difenil-2-picrilhidrazil (DPPH. Todas as polpas de frutas exibiram quantidades relevantes de polifenóis, no entanto, as de acerola, caju e ciriguela exibiram os mais elevados teores destes constituintes. As polpas de acerola, caju, ciriguela, goiaba, manga, pitanga e uva, diluídas em água, exibiram forte capacidade de seqüestro do radical DPPH (>70%, estatisticamente semelhante à do BHT e do ácido ascórbico. As polpas de cajá e graviola apresentaram moderada ação (50–70%, enquanto que as de tangerina e de abacaxi exibiram a menor ação, integrando, juntamente com a de maracujá, o grupo das polpas com fraca capacidade antioxidante (<50%. O extrato metanólico, obtido a partir dos resíduos resultantes da fi ltração das polpas diluídas em água, de todas as polpas, exceto a de uva e de cajá, cujo extrato aquoso exibiu forte capacidade de seqüestro, também apresentou forte ação antioxidante, estatisticamente semelhante à capacidade antioxidante do BHT e ácido ascórbico. Com base nos resultados obtidos, as polpas congeladas de frutas podem ser vistas com uma importante fonte de antioxidante dietético.

  16. Relação familiar da pessoa idosa com comprometimento da capacidade funcional

    Directory of Open Access Journals (Sweden)

    Luana Araújo dos Reis

    2015-01-01

    Full Text Available Objetivo: comprender la relación familiar de la persona adulta con comprometimiento de la capacidad funcional. Materiales y mé - todos: estudio exploratorio y descriptivo de abordaje cualitativo, fundamentado en el método de la historia oral, realizado con 15 adultos mayores asistidos por una unidad de salud de la familia, residentes con familiares y que presentaban comprometimiento de la capacidad funcional. Se recolectaron los datos en el período de marzo y abril del 2012, por medio de entrevistas en profundidad. Las categorías temáticas fueron: buena relación familiar, cambios en la relación familiar, sintiéndose un estorbo para la familia, sintiéndose abandonado por la familia. Resultado: el estudio ha revelado que las personas mayores con comprometimiento de la capacidad funcional vivencian sentimientos diversos que van desde la alegría porque son respetadas y tienen sus necesidades atendidas, hasta la tristeza y revuelta, por la adaptación negativa de la familia y el abandono de los hijos. Conclusión: la relación familiar de la persona mayor pasa por reajustes luego del comprometimiento de la capacidad funcional, lo que repercute significativamente en la dinámica de las relaciones. Desde esta comprensión, los profesionales de salud, sobre todo de enfermería, deben reconocer los retos a los que la familia afronta, orientarla y capacitarla para la atención a las demandas de cuidado presentadas por la persona mayor con comprometimiento de la capacidad funcional, y favorecer la realización del cuidado sin que esto desgaste las relaciones.

  17. Madurez de las capacidades de innovación en empresas colombianas

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    José Enrique Arias Pérez

    2014-01-01

    Full Text Available En la ciudad de Medellín (Colombia, se encuentran ubicadas algunas de las grandes empresas del país líderes en investigación, desarrollo e innovación; sin embargo, a la fecha son pocos los estudios realizados en función de las capacidades de innovación subyacentes a los buenos resultados en el desarrollo de nuevos y mejorados productos. El objetivo del articulo es determinar la madurez de las capacidades de innovación de cinco de estas empresas. Para ello, se utilizo el modele teórico de Essmann y Du Preez (2009, el cual se compone de tres dimensiones: constructo capacidad de innovación, escala de madurez, y constructo organizacional. En cuanto ala metodología, se construyo un índice de capacidad de innovación, y se calcularon los rangos de los tres niveles de madurez, usando el análisis clúster (Jung et ai, 2009; ello permitió clasificar a las empresas objeto de estudio. En cuanto a los resultados, se encontró que la totalidad de las empresas se encuentran en el nivel de madurez formalizado y predecible. En conclusión, si bien se han identificado e implementado las mejores practicas asociadas a cada capacidad de innovación, no se ha logrado que los individuos se apropien y hagan uso de manera sistemática de las mismas.

  18. Índices de resistência e pulsatilidade das artérias uterinas no primeiro e segundo trimestres de gestações normais Uterine arteries resistance and pulsatility indices at the first and second trimesters of normal pregnancies

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    Antonio Gadelha da Costa

    2010-06-01

    Full Text Available OBJETIVO: Determinar os índices de resistência (IR e pulsatilidade (IP nas artérias uterinas durante o primeiro e segundo trimestres de gestação. MATERIAIS E MÉTODOS: Realizamos estudo prospectivo longitudinal em 44 mulheres normais, da 8ª a 12ª e na 22ª semanas de gestação. A dopplervelocimetria foi realizada em ambas as artérias uterinas por meio do IR e do IP. Utilizamos volume de amostra de 1 a 2 mm, filtro de 50 a 70 Hz e ângulo de insonação abaixo de 60°. RESULTADOS: O IR e o IP da artéria uterina no primeiro trimestre foram maiores da 8ª a 12ª do que na 22ª semana de gestação. O IR e o IP da artéria uterina, no primeiro trimestre, com incisura foram de 0,83 ± 0,07 e 2,32 ± 0,79, e sem incisura, de 0,71 ± 0,16 e 1,61 ± 0,78, respectivamente. No segundo trimestre, o IR e o IP da artéria uterina com incisura foram de 0,59 ± 0,09 e 1,03 ± 0,32, e sem incisura, de 0,44 ± 0,09 e 0,63 ± 0,19, respectivamente. CONCLUSÃO: Estabelecemos os valores de referência do IR e do IP das artérias uterinas de gestantes normais no primeiro e segundo trimestres de gestação.OBJECTIVE: To determine uterine artery resistance index (RI and pulsatility index (PI during the first and second trimesters of pregnancy. MATERIALS AND METHODS: The authors developed a prospective longitudinal study with 44 healthy women at their 8th-12th and 22nd gestational weeks. Doppler velocimetry was utilized for evaluating RI and PI of both uterine arteries, with a sample volume of 1 to 2 mm, a 50-70 Hz filter, and an insonation angle < 60°. RESULTS: At 8th-12th gestational weeks, the uterine artery RI and PI were higher than at the 22nd gestational week. At the first trimester uterine artery RI and PI were, respectively, 0.83 ± 0.07 and 2.32 ± 0.79 with a notch, and 0.71 ± 0.16 and 1.61 ± 0.78, without a notch. At the second trimester, uterine artery RI and PI were, respectively, 0.59 ± 0.09 and 1.03 ± 0.32 with a notch, and 0.44 ± 0

  19. Influence of bladder outlet obstruction and detrusor contractility on residual urine in patients with benign prostatic hyperplasia

    Institute of Scientific and Technical Information of China (English)

    张鹏; 武治津; 高居忠

    2003-01-01

    Objective To study the relationship between the degree of bladder outlet obstruction (BOO), detrusor contractility and residual urine in patients suffering from benign prostatic hyperplasia (BPH).Methods In 181 patients with BPH, degree of BOO, detrusor contractility, residual urine caculated from cathetering combined with the difference between the filling and the voiding were recorded and analysized statistically using urodynamic technique.Results Residual urine increased when the detusor contractility was weakened (F=12.134, P=0.001). In patients wih severe BOO, there was no significant difference in residual urine (F=2.386, P=0.071).Conclusions Increased residual urine is mainly resulted from decreased detrusor contractility. BOO has no significant influence on residual urine. Some patients with normal or weakened detrusor contractility may have more residual urine

  20. Doppler de arterias uterinas en la predicción de trastornos hipertensivos del embarazo en un grupo de pacientes que asisten a tamizaje integrado en semana 11- 13 6/7 . En una unidad de alto riesgo obstétrico

    OpenAIRE

    González Rodríguez, Juan; Gómez, Marco Antonio

    2013-01-01

    Objetivo: Describir la utilidad diagnóstica y el poder de detección del índice de pulsatilidad anormal de las arterias uterinas durante el primer trimestre del embarazo en relación con la aparición de trastornos hipertensivos asociados al embarazo. Metodología: Estudio retrospectivo, longitudinal con alcances de tipo descriptivo de gestantes con embarazo único que se presentaron a control prenatal en obstetricia y ginecología y a quienes se les realizó tamizaje integrado entre las 11 y136/7 s...

  1. Enhancement by calcitonin gene-related peptide of non-contractile Ca2(+)-induced nicotinic receptor desensitization at the mouse neuromuscular junction.

    OpenAIRE

    Dezaki, K.; Kimura, I.; Tsuneki, H.; Kimura, M

    1996-01-01

    1. Nicotinic acetylcholine receptor (AChR)-operated non-contractile Ca2+ mobilization (unaccompanied by muscle contraction) depressed contractile Ca2+ mobilization (accompanied by muscle contraction) in mouse diaphragm muscles. In the process of nicotinic AChR desensitization, the enhancing role of calcitonin gene-related peptide (CGRP) on the non-contractile Ca2(+)-induced depression of contractile Ca2+ mobilization was investigated by measurement of Ca2(+)-aequorin luminescence in the prese...

  2. Função endotelial, perfusão uterina e fluxo central em gestações complicadas por Pré-Eclampsia Endothelial function, uterine perfusion and central Flow in pregnancies complicated by Preeclampsia

    Directory of Open Access Journals (Sweden)

    Augusto Henriques Fulgêncio Brandão

    2012-10-01

    Full Text Available FUNDAMENTO: A fisiopatologia da Pré-Eclampsia (PE é caracterizada por deficiência no processo de placentação, disfunção endotelial sistêmica e hiperfluxo do Sistema Nervoso Central (SNC. Do ponto de vista clínico, seria interessante determinar a ocorrência desses fenômenos antes do aparecimento das manifestações clínicas da doença, levantando a possibilidade de novos métodos de predição da PE. OBJETIVO: Comparar o processo de placentação, a função endotelial e o hiperfluxo do SNC em gestantes de alto risco para desenvolvimento de PE que posteriormente desenvolveram ou não a síndrome. MÉTODOS: Um total de 74 gestantes foi submetido ao exame de Dilatação Fluxo-Mediada (DFM da artéria braquial, dopplerfluxometria de artérias uterinas e oftálmica para avaliação da função endotelial, processo de placentação e hiperfluxo central, respectivamente. Os exames foram realizados entre 24 e 28 semanas de gestação e as pacientes foram acompanhadas até o puerpério para coleta de dados. RESULTADOS: Quinze pacientes tiveram a gestação complicada pela PE e 59 se mantiveram normotensas até o puerpério. Pacientes que subsequentemente desenvolveram PE apresentaram entre 24 e 28 semanas de gestação, maiores valores no índice de pulsatilidade das artérias uterinas e menores valores de DFM (p BACKGROUND: The physiopathology of Preeclampsia (PE is characterized by a deficiency in the process of placentation, systemic endothelial dysfunction and Central Nervous System (CNS hyperflow. From a clinical point of view, it would be interesting to determine the occurrence of these phenomena before the onset of clinical manifestations of the disease, raising the possibility of new methods for predicting PE. OBJECTIVE:Compare the process of placentation, endothelial function and CNS hyperflow in pregnant women at high risk for the development of PE who subsequently developed or not the syndrome. METHODS: A total of 74 pregnant women

  3. Sperm concentration on the intrauterine artificial insemination in swine Concentrações espermáticas na inseminação artificial intra-uterina suína

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    Joana Miller

    2009-12-01

    Full Text Available The aim of this work was to evaluate the efficiency of the intrauterine insemination (IUI in swine, considering the conception rate, farrowing rate, litter size (alive born pigs. For the IUI, the females had been insemination at 24 and 48 hours after the estrus detection, and the inseminating doses of 500 million, 1 billion, 1.5 billion and 2 billion spermatozoa in 20 mL extender had been used. The procedure of catheter insertion through the cervical canal was successfully performed in 97.9% of the females. The conception rate was 6.3% in the IUI. The farrowing rate in IUI was 87.2% but the farrowing rate was 100% for the sperm concentration of 500 million. Regarding the number of born pigs and alive born pigs observed in females inseminated with IUI, no significant difference was observed (p > 0.05. The concentration of 500 x 10(6 spermatozoa in 20 mL extender in the intrauterine insemination resulted in an optimal reproductive performance.Conduziu-se este estudo, com o objetivo de avaliar a eficiência da inseminação intra-uterina (IIU em suínos, considerando as taxas de retorno ao estro, aborto, parto, além do tamanho da leitegada (número de leitões nascidos e nascidos vivos. Na IIU, as fêmeas foram inseminadas nos tempos de 24 e 48 horas após a detecção do estro, utilizando-se as concentrações de 500 milhões, 1 bilhão, 1,5 bilhão e 2 bilhões de espermatozóides, em 20mL de diluente. A passagem do cateter de IIU através da cérvix foi possível em 97,9% das fêmeas. Foi realizado diagnóstico de retorno ao estro a partir do 18º dia e diagnóstico de gestação por ultrassonografia transcutânea entre o 28º e 30º dias após a inseminação. A taxa de retorno ao estro foi de 6,3% na IIU. A taxa de parto na IIU foi de 87,2%, sendo a taxa de parto para a concentração de 500 milhões de 100%. Com relação ao número de leitões nascidos totais e nascidos vivos, não houve diferenças, entre as diferentes concentra

  4. Desempenho reprodutivo de fêmeas suínas submetidas à inseminação artificial intra-uterina ou à tradicional Reproductive performance of sows submitted to intrauterine or tradicional artificial insemination

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    Paulo Eduardo Bennemann

    2007-12-01

    Full Text Available Foram utilizadas 298 fêmeas pluríparas Camborough 22® distribuídas em dois tratamentos: T1 (n=154, inseminação intra-uterina (IAU com dose inseminante (DI contendo 0,5 bilhão de espermatozóides em volume total de 20ml; e T2 (n=144, inseminação tradicional (IAT, com DI contendo 3,0 bilhões de espermatozóides em volume total de 90ml. Foi possível a realização da IAU em 98,1% das fêmeas. A presença de sangue na extremidade do cateter ou espiral da pipeta de IAU foi observada em 8,4% das fêmeas. As taxas de prenhez (TPr e de parto ajustada não diferiram (P>0,05 entre a IAU e IAT. O tamanho da leitegada (TL diferiu entre os tratamentos (PA total of 298 Camborough 22® sows was distributed in two treatments: T1 (n=154: intrauterine insemination (IUI with 0.5 billion sperms in 20ml total volume; T2 (n=144: traditional insemination (TAI with 3.0 billion sperms in 90ml total volume. It was possible to perform the IUI in 98.1% of sows. It was observed presence of blood on the catheter tip or pipete in 8.4% of IUI sows. The pregnancy (PR and adjusted farrowing rates did not differ (P>0.05 among treatments. Litter size (LS differ among treatments (P<0.05, being observed a reduction of 0.8 piglet in IUI. The blood presence in the IUI did not affect the PR significantly, but resulted in a reduction of 2.6 piglets per litter.

  5. A massa gorda de risco afeta a capacidade aeróbia de jovens adolescentes

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    Luís Massuça

    2013-12-01

    Full Text Available OBJETIVO: Estudar o comportamento do sexo e os efeitos da idade e da massa gorda sobre a capacidade aeróbia de jovens adolescentes. MÉTODOS: Os 621 estudantes do ensino secundário participantes no estudo (14 aos 17 anos; feminino: n = 329, idade, 15,84 ± 0,92 anos; masculino: n = 292, idade, 15,82 ± 0,87 anos foram avaliados em duas categorias: morfologia (altura, peso e % massa gorda - %MG e aptidão física (capacidade aeróbia. As medições antropométricas foram realizadas de acordo com o protocolo descrito por Marfell-Jones e a %MG foi calculada por bioimpedância. A avaliação da capacidade aeróbia foi realizada com o teste aeróbio de corrida - PACER, e VO2máx relativo foi calculado utilizando a equação de Léger. Os resultados das avaliações foram classificados de acordo com os valores normativos das tabelas de referência da bateria de testes FITNESSGRAM® As técnicas estatísticas utilizadas foram: 1 cálculo de frequências; 2 teste t de Student para amostras independentes; e 3 ANOVA two-way seguida do teste post-hoc HSD de Bonferroni. RESULTADOS: 1 existem diferenças significativas entre sexos no que se refere à %MG e ao VO2máx; 2 durante a adolescência, o VO2máx estabiliza nos rapazes e sofre um declínio nas moças; 3 independentemente do sexo, a classe de %MG e a idade cronológica têm um efeito significativo sobre a capacidade aeróbia; e 4 em jovens adolescentes, com %MG de risco, a redução da %MG para níveis saudáveis parece resultar na melhoria da capacidade aeróbia. CONCLUSÃO: O impacto da %MG na capacidade aeróbia, reforça a importância da educação física escolar na promoção da saúde cardiovascular.

  6. Indicadores antropométricos de estado nutricional como preditores de capacidade em idosos

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    Kleyton Trindade Santos

    2014-06-01

    Full Text Available INTRODUÇÃO: Trata-se de estudo seccional que analisou dados de pesquisa epidemiológica de base populacional e domiciliar conduzida em um município do nordeste do Brasil. OBJETIVO: Identificar os indicadores antropométricos de estado nutricional que apresentam melhor capacidade preditiva de capacidade funcional em idosos. MÉTODOS: A população foi constituída por 316 idosos que foram avaliados por meio de questionário contendo informações sociodemográficas, atividade física e condições de saúde, seguido de avaliação antropométrica. A capacidade funcional foi avaliada por meio das escalas de Katz e de Lawton e Brody para atividades básicas e instrumentais da vida diária, respectivamente. Foram utilizados como indicadores de estado nutricional, o índice de massa corporal, a área muscular do braço e o perímetro da panturrilha. Estimou-se modelos simples e múltiplos de regressão logística multinomial, tendo a capacidade funcional como variável dependente e os indicadores antropométricos como variáveis de interesse. RESULTADOS: Participaram do estudo 173 mulheres (54,7% e 143 homens (45,3%. Os resultados da regressão múltipla mostraram que o perímetro da panturrilha foi o único preditor de capacidade funcional em mulheres (≤ P25: OR = 5,77, p = 0,028, para dependência nas atividades instrumentais, enquanto que nos homens o índice de massa corporal foi o único indicador associado (baixo peso: OR = 11,36, p = 0,006; sobrepeso: OR = 22,06, p = 0,002; para dependência nas atividades básicas e instrumentais. CONCLUSÃO: Os resultados permitem concluir que os indicadores antropométricos preditores de capacidade funcional em idosos variam de acordo com o sexo, com o perímetro da panturrilha sendo mais adequada às mulheres e o índice de massa corporal aos homens.

  7. Effect of phorbol esters on contractile state and calcium flux in cultured chick heart cells

    International Nuclear Information System (INIS)

    Phorbol esters are potent tumor promoters that have been widely used in studies of transmembrane signaling because of their ability to activate protein kinase C. To study the effect of phorbol esters (and indirectly, the role of protein kinase C) on the cardiac muscle contractility, the authors examined the effects of phorbol myristate acetate (PMA) on contractile state, transmembrane 45Ca fluxes, and cytosolic free Ca concentration ([Ca]/sub i/) using spontaneously contracting cultured chick ventricular cells. PMA produced a concentration- and time-dependent decrease in the amplitude of cell motion [half maximum inhibitory concentration] with maximal effect observed at 1 μM. PMA (1 μM) reduced 45Ca uptake rate by 16 /plus minus/ 4% and the size of the rapidly exchangeable Ca pool by 11 /plus minus/ 2%, but did not alter the 45Ca efflux rate. In fura-2-loaded cells. PMA produced a decrease in [Ca]/sub i/ from 96 /plus minus/ 7 to 72 /plus minus/ 5 nM with a time course similar to that of alteration in contractile amplitude. These results indicate that PMA influences transsarcolemmal Ca uptake, and thus the excitation-contraction process, and suggest that protein kinase C may modulate myocardial Ca homeostassis and contractile state

  8. Four days of muscle disuse impairs single fiber contractile function in young and old healthy men

    DEFF Research Database (Denmark)

    Hvid, Lars Grøndahl; Suetta, Charlotte Arneboe; Aagaard, Per;

    2013-01-01

    The purpose of the study was to investigate the effects of 4 days of disuse (knee brace) on contractile function of isolated vastus lateralis fibers (n=486) from 11 young (24.3+/-0.9 yrs) and 11 old (67.2+/-1.0 yrs) healthy men having comparable levels of physical activity. Prior to disuse single...

  9. Effect of pH on contractile state and transsarcolemmal Ca flux in cultured heart cells

    International Nuclear Information System (INIS)

    The mechanism of pH-induced changes in cardiac contractile state was investigated using spontaneously beating cultured chick embryo ventricular cells. Cells were equilibrated in HEPES-buffered medium at pH 7.4 and then exposed to pH 6.0, 7.4 or 8.8 medium. pH-induced changes in Ca uptake were insensitive to 1 μM verapamil but were sensitive to changes in Na gradient. pH 6.0 inhibited and pH 8.8 stimulated Na/sub i/-dependent Ca uptake. Replacement of Na in the efflux medium with choline during 45Ca efflux did not affect the pH-induced changes in Ca efflux, suggesting that pH alters Ca efflux via sarcolemmal Ca-ATPase. Intracellular alkalinization or acidification produced by NH4Cl (20 mM) altered contractile state by +140% or -60%, respectively, but failed to alter significantly the 45Ca influx or efflux rates. The authors conclude that pH-induced alteration in contractile state is due in part to changes in transsarcolemmal Ca movement via Na/Ca exchange as well as sarcolemmal Ca pump activity. pH/sub i/-induced alteration in contractile state is due to changes in Ca movements within the cell and/or in Ca sensitivity of myofibrils

  10. Redundant mechanisms recruit actin into the contractile ring in silkworm spermatocytes.

    Directory of Open Access Journals (Sweden)

    Wei Chen

    2008-09-01

    Full Text Available Cytokinesis is powered by the contraction of actomyosin filaments within the newly assembled contractile ring. Microtubules are a spindle component that is essential for the induction of cytokinesis. This induction could use central spindle and/or astral microtubules to stimulate cortical contraction around the spindle equator (equatorial stimulation. Alternatively, or in addition, induction could rely on astral microtubules to relax the polar cortex (polar relaxation. To investigate the relationship between microtubules, cortical stiffness, and contractile ring assembly, we used different configurations of microtubules to manipulate the distribution of actin in living silkworm spermatocytes. Mechanically repositioned, noninterdigitating microtubules can induce redistribution of actin at any region of the cortex by locally excluding cortical actin filaments. This cortical flow of actin promotes regional relaxation while increasing tension elsewhere (normally at the equatorial cortex. In contrast, repositioned interdigitating microtubule bundles use a novel mechanism to induce local stimulation of contractility anywhere within the cortex; at the antiparallel plus ends of central spindle microtubules, actin aggregates are rapidly assembled de novo and transported laterally to the equatorial cortex. Relaxation depends on microtubule dynamics but not on RhoA activity, whereas stimulation depends on RhoA activity but is largely independent of microtubule dynamics. We conclude that polar relaxation and equatorial stimulation mechanisms redundantly supply actin for contractile ring assembly, thus increasing the fidelity of cleavage.

  11. Impaired pulmonary artery contractile responses in a rat model of microgravity: role of nitric oxide

    Science.gov (United States)

    Nyhan, Daniel; Kim, Soonyul; Dunbar, Stacey; Li, Dechun; Shoukas, Artin; Berkowitz, Dan E.

    2002-01-01

    Vascular contractile hyporesponsiveness is an important mechanism underlying orthostatic intolerance after microgravity. Baroreceptor reflexes can modulate both pulmonary resistance and capacitance function and thus cardiac output. We hypothesized, therefore, that pulmonary vasoreactivity is impaired in the hindlimb-unweighted (HLU) rat model of microgravity. Pulmonary artery (PA) contractile responses to phenylephrine (PE) and U-46619 (U4) were significantly decreased in the PAs from HLU vs. control (C) animals. N(G)-nitro-L-arginine methyl ester (10(-5) M) enhanced the contractile responses in the PA rings from both C and HLU animals and completely abolished the differential responses to PE and U4 in HLU vs. C animals. Vasorelaxant responses to ACh were significantly enhanced in PA rings from HLU rats compared with C. Moreover, vasorelaxant responses to sodium nitroprusside were also significantly enhanced. Endothelial nitric oxide synthase (eNOS) and soluble guanlyl cyclase expression were significantly enhanced in PA and lung tissue from HLU rats. In marked contrast, the expression of inducible nitric oxide synthase was unchanged in lung tissue. These data support the hypothesis that vascular contractile responsiveness is attenuated in PAs from HLU rats and that this hyporesponsiveness is due at least in part to increased nitric oxide synthase activity resulting from enhanced eNOS expression. These findings may have important implications for blood volume distribution and attenuated stroke volume responses to orthostatic stress after microgravity exposure.

  12. Appearance of contractile endothelin-B receptors in rat mesenteric arterial segments following organ culture

    DEFF Research Database (Denmark)

    Adner, M; Geary, G G; Edvinsson, L

    1998-01-01

    The aim of this study was to examine how different procedures for organ culture affect the expression of contractile endothelin(ET)-B receptors in a branch of the rat mesenteric artery. In fresh segments, ET-1 and ET-3 induced similar strong contractions, ET-1 being 20-fold more potent, whereas n...

  13. Effects of C-type natriuretic peptide on rat cardiac contractility

    OpenAIRE

    Brusq, Jean-Marie; Mayoux, Eric; Guigui, Laurent; Kirilovsky, Jorge

    1999-01-01

    Natriuretic peptide receptors have been found in different heart preparations. However, the role of natriuretic peptides in the regulation of cardiac contractility remains largely elusive and was, therefore, studied here.The rate of relaxation of electrically stimulated, isolated rat papillary muscles was enhanced (114.4±1.4%, P

  14. Protective Effect of Capsicum Frutescens on Contractile Reactivity of Streptozotocin-Diabetic Rats

    Directory of Open Access Journals (Sweden)

    F. Roghani-Dehkordi

    2005-07-01

    Full Text Available Introduction & Objective : Considering the higher incidence of atherosclerosis and cardiovascular disorders in diabetes mellitus, this study was conducted to evaluate the effect of oral one-month administration of red pepper (Capsicum frutescens on the contractile reactivity of isolated aorta in diabetic rats. Materials & Methods : For this purpose, male Wistar rats(n=32 were randomly divided into control, pepper-treated control, diabetic, and pepper-treated diabetic groups. For induction of diabetes, streptozotcin (STZ was intraperitoneally administered (60 mg/Kg. Pepper-treated groups received pepper mixed with standard pelleted food at a weight ratio of 1/15. After one month, contractile reactivity of aortic rings to KCl and noreadrenaline was determined using isolated tissue setup. Results : Serum glucose level showed a significant increase in diabetic group at 2nd and 4th weeks (P<0.001, while this increase was less marked in pepper-treated diabetic group at the 2nd week (P<0.05. In addition, the latter group showed a lower contraction to KCl (P<0.05 and noreadrenaline (P<0.05 as compared to diabetic group. Meanwhile, there was no significant difference between control and pepper-treated control groups regarding contractile reactivity. Conclusion : It can be concluded that oral administration of pepper for one month could attenuate the contractile responsiveness of the vascular system and may prevent the development of hypertension in diabetic rats.

  15. In vivo visualization and quantification of collecting lymphatic vessel contractility using near-infrared imaging.

    Science.gov (United States)

    Chong, Chloé; Scholkmann, Felix; Bachmann, Samia B; Luciani, Paola; Leroux, Jean-Christophe; Detmar, Michael; Proulx, Steven T

    2016-01-01

    Techniques to image lymphatic vessel function in either animal models or in the clinic are limited. In particular, imaging methods that can provide robust outcome measures for collecting lymphatic vessel function are sorely needed. In this study, we aimed to develop a method to visualize and quantify collecting lymphatic vessel function in mice, and to establish an in vivo system for evaluation of contractile agonists and antagonists using near-infrared fluorescence imaging. The flank collecting lymphatic vessel in mice was exposed using a surgical technique and a near-infrared tracer was infused into the inguinal lymph node. Collecting lymphatic vessel contractility and valve function could be easily visualized after the infusion. A diameter tracking method was established and the diameter of the vessel was found to closely correlate to near-infrared fluorescence signal. Phasic contractility measures of frequency and amplitude were established using an automated algorithm. The methods were validated by tracking the vessel response to topical application of a contractile agonist, prostaglandin F2α, and by demonstrating the potential of the technique for non-invasive evaluation of modifiers of lymphatic function. These new methods will enable high-resolution imaging and quantification of collecting lymphatic vessel function in animal models and may have future clinical applications. PMID:26960708

  16. Chronic clenbuterol treatment compromises force production without directly altering skeletal muscle contractile machinery.

    Science.gov (United States)

    Py, G; Ramonatxo, C; Sirvent, P; Sanchez, A M J; Philippe, A G; Douillard, A; Galbès, O; Lionne, C; Bonnieu, A; Chopard, A; Cazorla, O; Lacampagne, A; Candau, R B

    2015-04-15

    Clenbuterol is a β2 -adrenergic receptor agonist known to induce skeletal muscle hypertrophy and a slow-to-fast phenotypic shift. The aim of the present study was to test the effects of chronic clenbuterol treatment on contractile efficiency and explore the underlying mechanisms, i.e. the muscle contractile machinery and calcium-handling ability. Forty-three 6-week-old male Wistar rats were randomly allocated to one of six groups that were treated with either subcutaneous equimolar doses of clenbuterol (4 mg kg(-1) day(-1) ) or saline solution for 9, 14 or 21 days. In addition to the muscle hypertrophy, although an 89% increase in absolute maximal tetanic force (Po ) was noted, specific maximal tetanic force (sPo) was unchanged or even depressed in the slow twitch muscle of the clenbuterol-treated rats (P clenbuterol treatment significantly reduced the rate constant of force development and the slow and fast rate constants of relaxation in extensor digitorum longus muscle (P clenbuterol-treated animals demonstrated decreased amplitude after 14 days (-19%, P clenbuterol treatment reduces contractile efficiency, with altered contraction and relaxation kinetics, but without directly altering the contractile machinery. Lower Ca(2+) release during contraction could partially explain these deleterious effects. PMID:25656230

  17. Dopplervelocimetria das artérias uterinas na predição de complicações em gestações de baixo risco Uterine artery doppler velocimetry for the prediction of complications in low-risk pregnancies

    Directory of Open Access Journals (Sweden)

    Fabrício da Silva Costa

    2005-06-01

    Full Text Available OBJETIVO: determinar a validade da dopplervelocimetria das artérias uterinas na predição de complicações da gestação em população de nulíparas de baixo risco. MÉTODOS: foi conduzido estudo prospectivo que incluiu 45 pacientes primigestas, sem história de doenças crônicas. A dopplervelocimetria das artérias uterinas foi realizada entre 24 e 26 semanas, com determinação do índice de resistência (IR, índice de pulsatilidade (IP, relação S/D e a presença ou ausência de incisura na onda de velocidade de fluxo. Na análise estatística foi utilizado o teste de Mann-Whitney para amostras não paramétricas e o teste exato de Fisher foi utilizado na avaliação dos parâmentros qualitativos. RESULTADOS: complicações da gravidez foram observadas em 12 pacientes, com quatro casos de pré-eclâmpsia, um caso de recém-nascido pequeno para a idade gestacional (PIG, um caso de PIG e parto pré-termo (PPT, três casos de PPT, um caso de centralização fetal e dois casos de mecônio espesso no líquido amniótico no momento da resolução da gestação. Notamos que o IR (mediana 0,56 x 0,68, IP (mediana 0,98 x 1,29 e a relação S/D (mediana 2,2 x 2,9 estavam mais elevados no exame realizado entre a 24 e a 26ª semana nas pacientes que apresentaram qualquer complicação da gestação e não mostrou diferença nos casos de pré-eclâmpsia ou PIG. A presença de incisura bilateral apresentou sensibilidade de 100 e 90%, especificidade de 60,6 e 62,5%, valor preditivo positivo (VPP de 29,4 e 42,9%, valor preditivo negativo (VPN de 100 e 95,2% para a detecção de pré-eclâmpsia ou PIG e qualquer complicação da gestação, respectivamente. A dopplervelocimetria alterada apresentou sensibilidade de 83,3 e 83,3%, especificidade de 69,7 e 69,7%, VPP de 33,3 e 50,0%, VPN de 95,8 e 92,0% para a detecção de pré-eclâmpsia ou PIG e qualquer complicação da gestação, respectivamente. CONCLUSÃO: índices de alta impedância e a presen

  18. Efecto, en un modelo murino, de dos polisacáridos sulfatados sobre la producción de citoquinas por células NK uterinas y de sangre periférica

    Directory of Open Access Journals (Sweden)

    Angela Patricia Cadavid Jaramillo

    2000-02-01

    Full Text Available

    Las células asesinas naturales (NK uterinas son una subpoblación diferente a las de sangre periférica, expresan los marcadores CD56bright y CD16- y constituyen los linfocitos más abundantes de la decidua humana (70%. Estas células juegan un papel protagónico en el aloreconocimiento del feto, en el control de la invasión del trofoblasto y promueven el crecimiento de la unidad fetoplacentaria mediante la producción del factor estimulante de colonias de granulocitos y macrófagos y del factor estimulante de colonias de monocitos (CSF-1. En los ratones transgénicos Tg2978 deficientes en células NK se presenta una reducción de hasta un 66% en su viabilidad intrauterina y una disminución del 45% en el tamaño placentario señalando la estrecha relación de estas células con la biología de la reproducción.

    De otro lado existen pruebas directas e indirectas de que las células NK podrían ser responsables en algunos casos de falla del embarazo. Diversos tratamientos para el aborto recurrente espontáneo (ARE regulan negativamente el número y la actividad de las células NK como los glicosaminoglicanes sulfatados, y en especial la heparina, que bloquean in vitro su actividad lítica, tal como lo ha demostrado nuestro grupo. En estudios  clínicos de pacientes con ARE de causa inmunológica se observa un efecto benéfico de la combinación heparina-aspirina con o sin inmunizaciones con linfocitos, por lo cual se propone como una posible terapia de esta alteración. Se ha visto además que otro polisacárido sulfatado, el fucoidan, estimula la producción del factor de crecimiento hepático y

  19. Dietary exposure to ergot alkaloids decreases contractility of bovine mesenteric vasculature.

    Science.gov (United States)

    Egert, A M; Kim, D H; Schrick, F N; Harmon, D L; Klotz, J L

    2014-04-01

    Ergot alkaloids are hypothesized to cause vasoconstriction in the midgut, and prior exposure may affect the vasoactivity of these compounds. The objectives of this study were to profile vasoactivity of ergot alkaloids in bovine mesenteric artery (MA) and vein (MV) and determine if previous exposure to endophyte-infected tall fescue seed affected vasoactivity of ergocryptine (ERP), ergotamine (ERT), ergocristine (ERS), ergocornine (ERO), ergonovine (ERN), lysergic acid (LSA), ergovaline-containing tall fescue seed extract (EXT), and 5-hydroxytryptamine (5HT; serotonin). Ruminally cannulated Angus steers (n = 12; BW = 547 ± 31 kg) were paired by weight and randomly assigned to 6 blocks. Steers were ruminally dosed daily with 1 kg of either endophyte-infected (E+; 4.45 mg ergovaline/kg DM) or endophyte-free (E-; 0 mg ergovaline/kg DM) tall fescue seed for 21 d before slaughter. Branches of MA and MV supporting the cranial portion of the ileum were collected after slaughter on d 22, placed in a modified Krebs-Henseleit buffer on ice, cleaned, sectioned, and mounted in a multimyograph chamber. Contractile response was normalized to a maximum KCl response. Inner diameter (P = 0.04) and outer diameter (P = 0.02) of MA were smaller for E+ steers than E- steers. Maximum contractile responses to 120 mM KCl were not different between seed treatments in MA (P = 0.33; E-: 2.67 ± 0.43 g; E+: 3.33 ± 0.43 g) or MV (P = 0.26; E-: 2.01 ± 0.18 g; E+: 1.81 ± 0.18 g). Steers receiving E+ had a smaller (P < 0.01) MA contractile response than E- steers to ERP, ERT, ERS, ERO, ERN, EXT, and 5HT. Steers receiving E+ had a smaller (P < 0.05) MV contractile response than E- steers to ERP, ERT, ERS, ERN, EXT, and 5HT. Lysergic acid failed to induce a contractile response in MA and MV. The contractile response in MA and MV of E- steers produced by 5HT was very large. The EXT was the most potent (P < 0.05) agonist in MV and MA of E+ steers. These data showed that ergot alkaloids were

  20. Predicting changes in cardiac myocyte contractility during early drug discovery with in vitro assays

    International Nuclear Information System (INIS)

    Cardiovascular-related adverse drug effects are a major concern for the pharmaceutical industry. Activity of an investigational drug at the L-type calcium channel could manifest in a number of ways, including changes in cardiac contractility. The aim of this study was to define which of the two assay technologies – radioligand-binding or automated electrophysiology – was most predictive of contractility effects in an in vitro myocyte contractility assay. The activity of reference and proprietary compounds at the L-type calcium channel was measured by radioligand-binding assays, conventional patch-clamp, automated electrophysiology, and by measurement of contractility in canine isolated cardiac myocytes. Activity in the radioligand-binding assay at the L-type Ca channel phenylalkylamine binding site was most predictive of an inotropic effect in the canine cardiac myocyte assay. The sensitivity was 73%, specificity 83% and predictivity 78%. The radioligand-binding assay may be run at a single test concentration and potency estimated. The least predictive assay was automated electrophysiology which showed a significant bias when compared with other assay formats. Given the importance of the L-type calcium channel, not just in cardiac function, but also in other organ systems, a screening strategy emerges whereby single concentration ligand-binding can be performed early in the discovery process with sufficient predictivity, throughput and turnaround time to influence chemical design and address a significant safety-related liability, at relatively low cost. - Highlights: • The L-type calcium channel is a significant safety liability during drug discovery. • Radioligand-binding to the L-type calcium channel can be measured in vitro. • The assay can be run at a single test concentration as part of a screening cascade. • This measurement is highly predictive of changes in cardiac myocyte contractility

  1. Predicting changes in cardiac myocyte contractility during early drug discovery with in vitro assays

    Energy Technology Data Exchange (ETDEWEB)

    Morton, M.J., E-mail: michael.morton@astrazeneca.com [Discovery Sciences, AstraZeneca, Macclesfield, Cheshire SK10 4TG (United Kingdom); Armstrong, D.; Abi Gerges, N. [Drug Safety and Metabolism, AstraZeneca, Macclesfield, Cheshire SK10 4TG (United Kingdom); Bridgland-Taylor, M. [Discovery Sciences, AstraZeneca, Macclesfield, Cheshire SK10 4TG (United Kingdom); Pollard, C.E.; Bowes, J.; Valentin, J.-P. [Drug Safety and Metabolism, AstraZeneca, Macclesfield, Cheshire SK10 4TG (United Kingdom)

    2014-09-01

    Cardiovascular-related adverse drug effects are a major concern for the pharmaceutical industry. Activity of an investigational drug at the L-type calcium channel could manifest in a number of ways, including changes in cardiac contractility. The aim of this study was to define which of the two assay technologies – radioligand-binding or automated electrophysiology – was most predictive of contractility effects in an in vitro myocyte contractility assay. The activity of reference and proprietary compounds at the L-type calcium channel was measured by radioligand-binding assays, conventional patch-clamp, automated electrophysiology, and by measurement of contractility in canine isolated cardiac myocytes. Activity in the radioligand-binding assay at the L-type Ca channel phenylalkylamine binding site was most predictive of an inotropic effect in the canine cardiac myocyte assay. The sensitivity was 73%, specificity 83% and predictivity 78%. The radioligand-binding assay may be run at a single test concentration and potency estimated. The least predictive assay was automated electrophysiology which showed a significant bias when compared with other assay formats. Given the importance of the L-type calcium channel, not just in cardiac function, but also in other organ systems, a screening strategy emerges whereby single concentration ligand-binding can be performed early in the discovery process with sufficient predictivity, throughput and turnaround time to influence chemical design and address a significant safety-related liability, at relatively low cost. - Highlights: • The L-type calcium channel is a significant safety liability during drug discovery. • Radioligand-binding to the L-type calcium channel can be measured in vitro. • The assay can be run at a single test concentration as part of a screening cascade. • This measurement is highly predictive of changes in cardiac myocyte contractility.

  2. Mechanism underlying the reversal of contractility dysfunction in experimental colitis by cyclooxygenase-2 inhibition.

    Science.gov (United States)

    Khan, I; Oriowo, M A

    2006-03-01

    Inflammatory bowel diseases are associated with reduced colonic contractility and induction of cyclooxygenase-2. In this study a possible role of cyclooxygenase-2 in and the underlying mechanism of the reduced contractility were investigated in experimental colitis. The effects of meloxicam, a cyclooxygenase-2 selective inhibitor were examined on colonic contractility and MAP kinase p38 and ERK(1/2) expression. Colitis was induced in Sprague-Dawley male rats by intra-colonic instillation of trinitrobenzenesulphonic acid (TNBS; 40 mg/rat in 50 ethanol). The animals were divided into three groups. Group 1 (n=9) received meloxicam (3 mg/kg-day) gavage 1 h before and 1 day (Group 2) after induction of colitis. Group 3 (n=9) received phosphate buffered saline (PBS) in a similar manner and served as colitic control. The non colitic control animals received meloxicam in a similar manner. The animals were sacrificed after 5 days of treatment, colon was cleaned with PBS and colonic smooth muscle was obtained which was used in this study. Meloxicam treatment given 1 h before or 1 day after administration of colitis restored the reduced colonic contractility without affecting the sensitivity to carbachol. The levels of colonic smooth muscle IL-1beta mRNA, PGE(2), ERK(1/2), p38, malondialdehyde, myeloperoxidase activity and colonic mass were increased, whereas the body weight was decreased due to TNBS. The changes except colonic muscle mass and p38 expression were reversed by meloxicam treatment. These findings indicate that restoration of reduced colonic contractility by meloxicam is mediated by ERK(1/2), and that ERK(1/2) may serve as an important anti inflammatory target for treatment of colitis. PMID:16835710

  3. Cardiac-Specific Knockout of ETA Receptor Mitigates Paraquat-Induced Cardiac Contractile Dysfunction.

    Science.gov (United States)

    Wang, Jiaxing; Lu, Songhe; Zheng, Qijun; Hu, Nan; Yu, Wenjun; Li, Na; Liu, Min; Gao, Beilei; Zhang, Guoyong; Zhang, Yingmei; Wang, Haichang

    2016-07-01

    Paraquat (1,1'-dim ethyl-4-4'-bipyridinium dichloride), a highly toxic quaternary ammonium herbicide widely used in agriculture, exerts potent toxic prooxidant effects resulting in multi-organ failure including the lung and heart although the underlying mechanism remains elusive. Recent evidence suggests possible involvement of endothelin system in paraquat-induced acute lung injury. This study was designed to examine the role of endothelin receptor A (ETA) in paraquat-induced cardiac contractile and mitochondrial injury. Wild-type (WT) and cardiac-specific ETA receptor knockout mice were challenged to paraquat (45 mg/kg, i.p.) for 48 h prior to the assessment of echocardiographic, cardiomyocyte contractile and intracellular Ca(2+) properties, as well as apoptosis and mitochondrial damage. Levels of the mitochondrial proteins for biogenesis and oxidative phosphorylation including UCP2, HSP90 and PGC1α were evaluated. Our results revealed that paraquat elicited cardiac enlargement, mechanical anomalies including compromised echocardiographic parameters (elevated left ventricular end-systolic and end-diastolic diameters as well as reduced factional shortening), suppressed cardiomyocyte contractile function, intracellular Ca(2+) handling, overt apoptosis and mitochondrial damage. ETA receptor knockout itself failed to affect myocardial function, apoptosis, mitochondrial integrity and mitochondrial protein expression. However, ETA receptor knockout ablated or significantly attenuated paraquat-induced cardiac contractile and intracellular Ca(2+) defect, apoptosis and mitochondrial damage. Taken together, these findings revealed that endothelin system in particular the ETA receptor may be involved in paraquat-induced toxic myocardial contractile anomalies possibly related to apoptosis and mitochondrial damage. PMID:26089164

  4. Método para valorar la concentración y la capacidad antioxidante del ácido úrico

    OpenAIRE

    Campos, Carlos; Guzmán, Rodrigo; López Fernández, María Encarnación; Casado Moragón, Ángela

    2010-01-01

    La presente invención se refiere a un método para valorar la concentración y la capacidad antioxidante del ácido úrico en muestras ya sean biológicas, en muestras procedentes de la industria alimentaria, en cosméticos ... Además mediante la presente invención se permite determinar la capacidad antioxidante sin la contribución del ácido úrico y la capacidad antioxidante total de una muestra.

  5. Estudio de la integración visuomotora en niños con altas capacidades intelectuales

    OpenAIRE

    Gaona Bosque, Sara

    2011-01-01

    A lo largo de los años diversos autores han tratado de demostrar que los niños superdotados -actualmente denominados como niños con “altas capacidades intelectuales”- además de tener una mayor capacidad intelectual destacan también por poseer otras habilidades superiores, como por ejemplo, mayor desarrollo cognitivo, mayor capacidad creativa, niveles superiores de percepción, etc. En el presente estudio tratamos de determinar si también poseen una mejor integración visuomotora. Entendiendo la...

  6. Antecedentes estratégicos de la capacidad de innovación empresarial y sus efectos en los resultados

    OpenAIRE

    Fidel Criado, Pilar

    2016-01-01

    La presente tesis doctoral propone un modelo teórico sobre los antecedentes estratégicos que influyen en la capacidad de innovación de la empresa y su relación con los resultados empresariales. El modelo se construye desde una aproximación integradora de teorías y enfoques que une la teoría de recursos y capacidades y su extensión al enfoque de las capacidades dinámicas y el enfoque basado en el conocimiento, el enfoque al marketing, la teoría del aprendizaje organizacional y la Lógica Domina...

  7. Determinantes de la capacidad de innovación en PYMES regionales Determinants of innovativeness in regional SMES

    OpenAIRE

    Carlos Maria Fernández-Jardón

    2013-01-01

    DOI: 10.5902/198346597689La capacidad de innovación es una competencia distintiva que permite innovar y, en consecuencia, potencia la competitividad, especialmente en las pequeñas y medianas empresas (PYME). Dicha capacidad se ve condicionada por factores internos y externos. Este artículo analiza como combina la empresa dichos factores para mejorar la capacidad de innovación. Por un lado, la empresa genera una competencia distintiva denominada gestión de los recursos humanos y tecnológicos a...

  8. Avaliação da capacidade funcional e pulmonar em pacientes pediátricos transplantados renais

    OpenAIRE

    Renata Salatti Ferrari; Camila Wohlgemuth Schaan; Karina Cerutti; Juliana Mendes; Clotilde Druck Garcia; Mariane Borba Monteiro; Janice Luisa Lukrafka

    2013-01-01

    INTRODUÇÃO: Pacientes pediátricos submetidos a transplante renal podem apresentar alterações na função pulmonar, bem como na capacidade funcional para o exercício. OBJETIVO: Avaliar a capacidade funcional e capacidade pulmonar de crianças e adolescentes submetidas a transplante renal. MÉTODO: Foram avaliadas crianças e adolescentes com idade entre 6 e 18 anos em acompanhamento no ambulatório de Nefrologia do Hospital da Criança Santo Antônio, Santa Casa de Porto Alegre-RS, Brasil, no período ...

  9. Capacidad de carga de las anclas de plataformas petroleras. // Load capacity of oil platforms anchors.

    Directory of Open Access Journals (Sweden)

    J. García de la Figal Costales

    2001-07-01

    Full Text Available Se determina la capacidad de carga de las anclas de solicitación vertical, empleadas en la fijación de las plataformasperforadoras de petróleo de aguas profundas. Para ello, se desarrollan varios modelos del proceso de retención del ancla enel fango del fondo marino por medio del método de los Elementos Finitos. Se analizan con ellos, la influencia de variosparámetros en la capacidad de carga.Palabras claves: ancla, petróleo, elementos finitos, plataformas petroleras._________________________________________________________________________________AbstractThe load capacity of anchors for vertical fixation used as petroleum perforators platforms on deep waters are determined.Several models of retention process of the anchor are developed by means of Finite Elements Method and the influence ofseveral parameters in the load capacity are analized.Key words: anchors, petroleum, Finite Elements Method (FEM.

  10. Avaliação da capacidade na rede ferroviária portuguesa

    OpenAIRE

    Ferreira, Ricardo José Teixeira

    2014-01-01

    Tendo em conta a escassez de combustíveis fósseis e o aumento do custo destes, torna-se importante o estudo e desenvolvimento de alternativas ao transporte rodoviário. O transporte ferroviário surge, assim, como uma possível opção. Esta dissertação apresenta os diferentes tipos de capacidade de uma rede ferroviária, os fatores que a influenciam e métodos de obtenção da mesma. Em relação aos métodos de obtenção da capacidade, esta dissertação foca-se especialmente no método UIC 406, que é s...

  11. Variación de la capacidad portante de suelos granulares contaminados con hidrocarburos

    OpenAIRE

    Fernandez Serrano, Roberto; Martínez Santamaría, José Manuel; Rogel Quesada, Juan Manuel

    2012-01-01

    El trabajo presenta una investigación sobre la variación de las propiedades geotécnicas básicas de una Arena de Miga (de Madrid) contaminada con hidrocarburos ligeros. Se deducen las siguientes conclusiones: (1) comportamiento particular frente a la compactación; (2) Disminución de la resistencia cortante drenada y (3) Variación de la capacidad portante de los suelos contaminados.

  12. Capacidad cardio-respiratoria de niños escolares que viven a moderada altitud

    Directory of Open Access Journals (Sweden)

    ROSSANA GÓMEZ-CAMPOS

    2014-04-01

    Full Text Available Objetivo: Determinar la capacidad cardio-respiratoria de niños escolares que viven a moderada altitud. Pacientes y Método: Fueron seleccionados 795 niños (394 niños y 401 niñas de escuelas públicas del área urbana de moderada altitud (2.320 msnm de Arequipa, Perú. Se evaluaron variables antropométricas (masa corporal, estatura, porcentaje de grasa y capacidad cardio-respiratoria a través del test indirecto de Course Navette, considerando las siguientes categorías: deficiente, malo, aceptable, bueno, muy bueno y excelente. Resultados: Los resultados muestran diferencias significativas en todas las categorías (p < 0,05. Se verificó que los valores medios de las categorías malo y deficiente en ambos sexos son inferiores a los de la categoría aceptable (p < 0,05. Se determinó que el 19% de niños y 21% de niñas mostraron bajo nivel de capacidad cardio-respiratoria, además se verificó correlación negativa baja con la categoría sobrepeso (r = -0,20 a -0,22 y moderada con la obesidad (r = -0,39 a -0,42 en ambos sexos. Conclusiones: Existen bajos niveles de capacidad cardio-respiratoria en niños de ambos sexos niños escolares que viven a moderada altitud, que se correlaciona negativamente con el exceso de peso corporal. Los resultados sugieren que uno de cada 5 niños se encuentra con posibilidades de padecer algún evento de índole cardiovascular.

  13. ¿Contribuyen las asignaturas de finanzas al desarrollo de la capacidad gerencial del administrador?

    Directory of Open Access Journals (Sweden)

    Luis M. Prada B.

    1996-12-01

    Full Text Available En este artículo el autor presenta los resultados de un trabajo de investigación sobre el aporte de las asignaturas de finanzas al desarrollo de capacidades gerenciales de innovación, liderazgo, comunicación e integridad ética, entre estudiantes de la carrera de Administración en la Universidad Nacional de Colombia. La investigación se realizó de dos maneras: una teórica, consistente en verificar que los programas de esas asignaturas mencionaran de manera explícita en los objetivos, contenidos, metodologías y formas de evaluación, la intención de propiciar el desarrollo de dichas capacidades. La otra, práctica, consistente en conocer la opinión de los estudiantes sobre el aporte real que hicieron esas asignaturas a su desarrollo personal y profesional, con respecto a las mencionadas capacidades. Según los estudiantes, los programas de las cuatro asignaturas obligatorias de finanzas no integran, ni en su enunciado, ni en su desarrollo, principios y estrategias educativas, elementos que promuevan el desarrollo de las mencionadas características gerenciales.

  14. Uso público y capacidad de carga perceptual en espacios naturales protegidos

    Directory of Open Access Journals (Sweden)

    Felipe Leco Berrocal

    2013-01-01

    Full Text Available Este trabajo muestra algunos de los resultados de la investigación realizada en el Proyecto «Las áreas de uso público en la Reserva de la Biosfera de Monfragüe. Accesibilidad y capacidad de carga» (Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica 2004-2007. Orden MAM/321/207. Expte. 024/SGTB/2007/4.1. En líneas generales, se presenta un estudio de las principales áreas de uso público desde la perspectiva de la capacidad de carga, ya que dichas áreas polarizan por regla general los flujos de usuarios, las inversiones y son el eje principal sobre el que descansa la afluencia de usuarios al Parque, por ello es necesario evaluar no sólo la capacidad de carga sino también la intensidad de su uso.

  15. Stretch-induced increase in cardiac contractility is independent of myocyte Ca2+ while block of stretch channels by streptomycin improves contractility after ischemic stunning

    OpenAIRE

    Rhodes, Samhita S.; Camara, Amadou K.S.; Aldakkak, Mohammed; Heisner, James S.; Stowe, David F

    2015-01-01

    Stretching the cardiac left ventricle (LV) enhances contractility but its effect on myoplasmic [Ca2+] is controversial. We measured LV pressure (LVP) and [Ca2+] as a function of intra-LV stretch in guinea pig intact hearts before and after 15 min global stunning ± perfusion with streptomycin (STM), a stretch-activated channel blocker. LV wall [Ca2+] was measured by indo-1 fluorescence and LVP by a saline-filled latex balloon inflated in 50 μL steps to stretch the LV. We implemented a mathemat...

  16. El derecho a la salud: una visión a partir del enfoque de capacidades

    Directory of Open Access Journals (Sweden)

    Alba L. Vélez Arango

    2015-02-01

    Full Text Available Objetivos: 1 identificar en las tesis libertarias y en la tradición liberal igualitaria de John Rawls la aceptación y/o rechazo de la protección de la salud como requisito de justicia social. 2 Analizar en los idearios teóricos de Amartya Sen y Martha Nussbaum elementos a favor de la protección de la salud y su goce efectivo como parte de los derechos que deben reconocerse por la sociedad, como respuesta a cuestiones de justicia social. Metodología: investigación de carácter filosófico, analizó críticamente los fundamentos morales y políticos que sustentan el reconocimiento del derecho a la salud como un requisito de justicia social. Resultados: en el proyecto rawlsiano la estructura básica de la sociedad debe distribuir ciertos “bienes primarios”, cosas que se presume que todo ser racional desea para poder desarrollar los planes de vida. Amartya Sen y Martha Nussbaum proponen el enfoque de las capacidades humanas básicas; el primero lo justifica en torno a la libertad y la segunda como requisitos básicos para una vida digna. Nussbaum justifica una lista de diez capacidades, dentro de las cuales incluye la vida, la salud física y la integridad física; todas relacionadas directamente con la salud. Así la tutela en salud se torna en condición necesaria de justicia social. El enfoque de las capacidades introduce la idea de un umbral para cada capacidad, por debajo del cual se considera que los ciudadanos no pueden funcionar de un modo auténticamente humano. Conclusiones: la protección de la salud instrumentalizada a través del sistema de salud es condición necesaria para el desarrollo de la vida que cada individuo tiene razones para valorar. Su protección debe darse dentro de los umbrales mínimos de justicia y la meta social debería entenderse en el sentido de lograr que los ciudadanos se sitúen por encima de este conjunto de capacidades entre las que se encuentra la salud.

  17. CAP_USO: Um sistema especialista para determinação da capacidade de uso da terra

    OpenAIRE

    Monica Luri Giboshi; Luiz Henrique Antunes Rodrigues; Francisco Lombardi Neto

    1999-01-01

    O objetivo deste trabalho foi desenvolver um sistema especialista (CAP_USO) para determinar a capacidade de uso da terra, visando auxiliar e dar mais agilidade ao processo de tomada de decisão para o planejamento do uso da terra. O sistema foi desenvolvido utilizando um software conhecido como C Language Integrated Production System (CLIPS), desenvolvido pela National Aeronautics and Space Administration (Nasa). Para determinar a capacidade de uso, foram considerados doze fatores limitantes a...

  18. Associação entre a Incisura Diastólica das Artérias Uterinas e a Histologia do Leito Placentário em Grávidas com Pré-eclâmpsia Association between Diastolic Notch of Uterine Artery and the Histology of the Placental Bed in Pregnant Women with Preeclampsia

    Directory of Open Access Journals (Sweden)

    Regina Amélia Lopes Pessoa de Aguiar

    2001-08-01

    Full Text Available Objetivo: avaliar a associação entre a presença da incisura diastólica nas artérias uterinas maternas e as alterações histopatológicas dos vasos útero-placentários. Métodos: estudo transversal incluindo 144 pacientes com gestação única interrompida por via abdominal entre a 27ª e a 41ª semana. Destas, 84 gestações estavam associadas à pré-eclâmpsia e 60 não apresentaram intercorrências clínicas. Neste grupo realizou-se dopplerfluxometria de ambas as artérias uterinas e biópsia do leito placentário. Resultados: das 144 pacientes, 88 (61% tiveram o fragmento da biópsia considerado representativo do leito placentário. A incisura diastólica estava presente em 40 (70% dos casos de alterações fisiológicas inadequadas e ausente em 28 (90% dos casos de alterações fisiológicas presentes (p=0,05. A dopplerfluxometria apresentou sensibilidade de 70%, especificidade de 90% e valores preditivos positivo e negativo de 44 e 97%, respectivamente. A associação entre a presença de incisura diastólica bilateral das artérias uterinas e arteriopatia decidual também foi significativa (dos 25 casos de arteriopatia decidual a incisura estava presente em 24, p=0,05. A sensibilidade da dopplerfluxometria foi de 96%, especificidade de 70% e valores preditivos positivo e negativo de 26 e 99%, respectivamente, ao passo que para a arteriolosclerose a dopplerfluxometria apresentou sensibilidade de 80%, especificidade de 55% e valores preditivos positivo e negativo de 17 e 96%, respectivamente. Conclusões: a incisura diastólica nas artérias uterinas maternas é indicador seguro de vasculopatia no leito placentário. A adequada invasão trofoblástica do leito placentário, revelada por histologia típica de alterações fisiológicas, resulta na ausência de incisura diastólica bilateral das artérias uterinas maternas.Purpose: to evaluate the association between the presence of diastolic notch in the maternal uterine arteries, and

  19. UN MODELO CAUSAL DE COMPETITIVIDAD EMPRESARIAL PLANTEADO DESDE LA VBR: CAPACIDADES DIRECTIVAS, DE INNOVACIÓN, MARKETING Y CALIDAD

    Directory of Open Access Journals (Sweden)

    Martínez Santa María, R.

    2010-01-01

    Full Text Available El propósito de esta investigación es desarrollar un modelo explicativo de la competitividad empresarial a partir de factores internos a la empresa. Para ello se identifican los factores de competitividad más importantes en el caso de las empresas manufactureras vascas, así como las variables que permiten caracterizarlos, utilizando técnicas cualitativas basadas en entrevistas con directivos. Los factores resultantes son las capacidades directivas, las capacidades de innovación, las capacidades de marketing y las capacidades de calidad. Tras analizar estos factores bajo la Visión de la empresa Basada en Recursos (VBR, y definir sus escalas de medida, se plantea un modelo causal que relaciona cada uno de ellos con la competividad de la empresa. Este modelo otorga una importancia especial a las capacidades directivas al plantear este factor como precedente del resto de capacidades de la empresa. El modelo es contrastado a través de la técnica de modelado con ecuaciones estructurales utilizando una muestra representativa de la población de empresas manufactureras vascas formada por 861 empresas.

  20. Effect of a crude sulfated polysaccharide from Halymenia floresia (Rhodophyta on gastrointestinal smooth muscle contractility

    Directory of Open Access Journals (Sweden)

    José Ronaldo Vasconcelos Graça

    2011-10-01

    Full Text Available The aim of this work was to study the effect of Halymenia floresia (Hf on duodenum contractility, and on experimental protocols of gastric compliance (GC in rats. Fraction Hf2s exhibited a concentration-dependent myocontractile effect (EC50 12.48 µg/ml, and an inhibitory effect after consecutive washing. The contractile response promoted by Hf2s in the duodenum strips was completely inhibited by verapamil, and the effects were prevented in the presence of Ca2+-free medium. The pretreatment with atropine prevented the Hf2s myocontractile effect. Hf2s was also capable to decrease the GC (from 3.8±0.06 to 3.4±0.13 ml, P<0.05, which did not return to basal levels after more 50 min of observation. These results indicated that the algal polysaccharide possessed in vitro and in vivo gastrointestinal effects.

  1. The Fiber Contractility and Cytoskeleton Losses in Space are Less Pronounced in Mongolian Gerbils

    Science.gov (United States)

    Lipets, E. N.; Ponomareva, E. V.; Ogneva, I. V.; Vikhliantsev, I. M.; Karaduleva, E. V.; Kartashkina, N. L.; Kuznetsov, S. L.; Podlubnaia, Z. A.; Shenkman, B. S.

    2008-06-01

    This work was purposed on the comparison of space flight effects on m. soleus and m. tibialis anterior of Mongolian gerbils. The animals have been flown onboard biosatellite Foton-M3 for 12 days. Contractile properties of single skinned muscle fibers were studied. It was revealed that diameter of m. soleus skinned fibers and maximal isometric tension were decreased by 19.7% and 21.8% respectively. The Ca-sensitivity reduction wasn't significant, that was in accordance with absence of changes of titin and nebulin relative content in soleus and minor manifestations in slow-to-fast fiber ratio (9%, p<0.05). There weren't observed significant changes of the same parameters in m. tibialis anterior. Ultimately the fiber contractility and cytoskeleton losses in space are less pronounced in Mongolian gerbils than in rats.

  2. BK channel activation by NS11021 decreases excitability and contractility of urinary bladder smooth muscle

    DEFF Research Database (Denmark)

    Layne, Jeffrey J; Nausch, Bernhard; Olesen, Søren-Peter;

    2009-01-01

    activation of BK channels has the converse effect of reducing UBSM excitability and contractility. Here, we have sought to investigate this possibility by using the novel BK channel opener NS11021. NS11021 (3 microM) caused an approximately threefold increase in both single BK channel open probability (P......Large-conductance Ca(2+)-activated potassium (BK) channels play an important role in regulating the function and activity of urinary bladder smooth muscle (UBSM), and the loss of BK channel function has been shown to increase UBSM excitability and contractility. However, it is not known whether......(o)) and whole cell BK channel currents. The frequency of spontaneous action potentials in UBSM strips was reduced by NS11021 from a control value of 20.9 + or - 5.9 to 10.9 + or - 3.7 per minute. NS11021 also reduced the force of UBSM spontaneous phasic contractions by approximately 50%, and this force...

  3. Measuring the Contractile Response of Isolated Tissue Using an Image Sensor

    Directory of Open Access Journals (Sweden)

    David Díaz-Martín

    2015-04-01

    Full Text Available Isometric or isotonic transducers have traditionally been used to study the contractile/relaxation effects of drugs on isolated tissues. However, these mechanical sensors are expensive and delicate, and they are associated with certain disadvantages when performing experiments in the laboratory. In this paper, a method that uses an image sensor to measure the contractile effect of drugs on blood vessel rings and other luminal organs is presented. The new method is based on an image-processing algorithm, and it provides a fast, easy and non-expensive way to analyze the effects of such drugs. In our tests, we have obtained dose-response curves from rat aorta rings that are equivalent to those achieved with classical mechanic sensors.

  4. Myosin-II dependent cell contractility contributes to spontaneous nodule formation of mesothelioma cells

    CERN Document Server

    Tárnoki-Zách, Julia; Méhes, Elod; Paku, Sándor; Neufeld, Zoltán; Hegedus, Balázs; Döme, Balázs; Czirok, Andras

    2015-01-01

    We demonstrate that characteristic nodules emerge in cultures of several malignant pleural mesothelioma (MPM) cell lines. Instead of excessive local cell proliferation, the nodules arise by Myosin II-driven cell contractility. The aggregation process can be prevented or reversed by suitable pharmacological inhibitors of acto-myosin contractility. A cell-resolved elasto-plastic model of the multicellular patterning process indicates that the morphology and size of the nodules as well as the speed of their formation is determined by the mechanical tension cells exert on their neighbors, and the stability of cell-substrate adhesion complexes. A linear stability analysis of a homogenous, self-tensioned Maxwell fluid indicates the unconditional presence of a patterning instability.

  5. Cell contractility facilitates alignment of cells and tissues to static uniaxial stretch

    CERN Document Server

    Rens, Elisabeth G

    2016-01-01

    During animal development and homeostasis, the structure of tissues, including muscles, blood vessels and connective tissues adapts to mechanical strains in the extracellular matrix (ECM). These strains originate from the differential growth of tissues or forces due to muscle contraction or gravity. Here we show using a computational model that by amplifying local strain cues, active cell contractility can facilitate and accelerate the reorientation of single cells to static strains. At the collective cell level, the model simulations show that active cell contractility can facilitate the formation of strings along the orientation of stretch. The computational model is based on a hybrid cellular Potts and finite-element simulation framework describing a mechanical cell-substrate feedback, where: 1) cells apply forces on the ECM, such that 2) local strains are generated in the ECM, and 3) cells preferentially extend protrusions along the strain orientation. In accordance with experimental observations, simulat...

  6. An Estimating Method of Contractile State Changes Come From Continuous Isometric Contraction of Skeletal Muscle

    Energy Technology Data Exchange (ETDEWEB)

    Park, H.J.; Lee, S.J. [Wonkwang University, Iksan (Korea)

    2003-01-01

    In this study was proposed that a new estimating method for investigation of contractile state changes which generated from continuous isometric contraction of skeletal muscle. The physiological changes (EMG, ECG) and the psychological changes by CNS(central nervous system) were measured by experiments, while the muscle of subjects contracted continuously with isometric contraction in constant load. The psychological changes were represented as three-step-change named 'fatigue', 'pain' and 'sick(greatly pain)' from oral test, and the method which compared physiological change with psychological change on basis of these three steps was developed. The result of analyzing the physiological signals, EMG and ECG signal changes were observed at the vicinity of judging point in time of psychological changes. Namely, it is supposed that contractile states have three kind of states pattern (stable, fatigue, pain) instead of two states (stable, fatigue). (author). 24 refs., 7 figs.

  7. Distribuição dos depósitos de glicogênio durante a odontogênese intra-uterina em Calomys callosus = Distribution of glycogen storage during odontogenesis in fetal development in Calomys callosus

    Directory of Open Access Journals (Sweden)

    Vieira, Andreia Espíndola

    2006-01-01

    Full Text Available As células animais estocam carboidratos na forma de glicogênio como fonte de energia. Esses depósitos ocorrem amplamente nos tecidos embrionários para disponibilização rápida de energia, principalmente durante a diferenciação e o desenvolvimento das células. O presente trabalho avaliou a distribuição de depósitos de glicogênio durante a odontogênese intra-uterina em Calomys callosus utilizando fetos com 12 a 20 dias, sendo 5 fetos de cada dia. As cabeças dos fetos foram removidas e fixadas em formaldeído 10% em PBS. Os espécimes foram processados para inclusão em glicol metacrilato (Leica Historesin, sendo aqueles com mais de 15 dias previamente descalcificados em EDTA. Para cada dia de desenvolvimento obteve-se cortes semi-seriados com 3 μm de espessura. As lâminas foram divididas em 2 grupos, sendo um grupo submetido à reação P. A. S. e o outro submetido à amilase salivar e em seguida à reação P. A. S. (grupo controle. Ao microscópio de luz foi observada, nas fases iniciais da odontogênese (12-15 dias, reação P. A. S. mais evidente nas células ectomesenquimais e aos 16-17 dias a reação predominou na região do folículo dental. Aos 18-19 dias a reação intensificou-se no retículo estrelado e aos 20 dias nas células do epitélio interno e na papila dental. De acordo com a metodologia empregada e com os resultados obtidos, concluímos que ocorre variação dos depósitos de glicogênio nas diferentes regiões do germe dental ao longo da odontogênese e que o Calomys callosus representa um modelo biológico para estudos da odontogênese.

  8. Embolização das artérias uterinas com partículas de PVA-PVAC esférico como preparo para posterior ressecção cirúrgica de miomas

    Directory of Open Access Journals (Sweden)

    Juraci Ghiaroni

    2013-10-01

    Full Text Available OBJETIVO: avaliar a utilização de uma nova partícula de polivinil álcool e polivinil acetato (PVA-PVAc esférica, para embolização das artérias uterinas, em pacientes portadoras de mioma, com indicação cirúrgica. MÉTODOS: doze pacientes foram submetidas à embolização de miomas uterinos com partículas de PVA-PVAc. Três a nove meses depois, realizou-se uma laparotomia com miomectomia. Analisaram-se os seguintes parâmetros: volume do útero e do maior mioma; concentrações do hormônio folículo estimulante e de hemoglobina; sangramento menstrual (número de dias e de absorventes utilizados, sinais e sintomas antes do tratamento, após a embolização e após a miomectomia. RESULTADOS: a média de idade foi 37 anos e a média do volume uterino, previamente ao tratamento, de 939,3cc. Três anos após a embolização, observou-se diminuição do volume uterino (p=0,0005. Houve melhora na concentração de hemoglobina (p= 0,0004, com elevação após a embolização, sem variação subsequente à miomectomia. Não ocorreu variação significante do hormônio folículo estimulante, (p=0,17. Não foi constatado nenhum caso de falência ovariana, mas uma das pacientes apresentou atrofia de endométrio. Duas pacientes engravidaram, com bons indicadores obstétricos. Quanto aos sinais e sintomas, houve melhora após a embolização, que se manteve após a miomectomia. CONCLUSÃO: a embolização arterial com partículas de PVA-PVAc esférico mostrou-se promissora no preparo para uma intervenção cirúrgica com retirada dos miomas, pois, associou-se à redução do volume uterino, à diminuição do sangramento operatório e tornou possível a utilização de incisões menores, aumentando a chance de preservação do útero.

  9. Desempenho reprodutivo de fêmeas suínas inseminadas pela técnica intra-uterina ou tradicional Reproductive performance of swine females inseminated by intrauterine or traditional technique

    Directory of Open Access Journals (Sweden)

    Djane Dallanora

    2004-08-01

    Full Text Available O desempenho reprodutivo de fêmeas suínas foi avaliado com o objetivo de comparar duas técnicas de inseminação. Foram inseminadas, em delineamento inteiramente casualizado, 608 fêmeas com ordem de parto de dois a quatro, em dois tratamentos: inseminação intra-uterina com 1,5 bilhão de espermatozóides em 60 mL e inseminação tradicional, com 3 bilhões em 90 mL. Foi possível introduzir o cateter intra-uterino em 97,4% das fêmeas e houve sangramento em 9,5%, as quais apresentaram retorno ao estro superior (pThe reproductive performance of sows was evaluated aiming at the comparison of two insemination techniques. In a completely randomized design, 608 females of 2-4 parities were inseminated in two treatments: intrauterine with 1.5x10(9 spermatozoa/60 mL or traditional insemination with 3x10(9 spermatozoa/90 mL. It was possible to insert the intrauterine catheter in 97.4% of females and bleeding was observed in 9.5%, which had a higher probability to repeat estrus (p<0.05. The percentage of semen backflow volume, during two hours after insemination, was significantly higher (p<0.05 in intrauterine insemination than traditional, whereas sperm backflow percentage was similar. The sperm backflow percentage did not influence the farrowing rate and the litter size. There was no difference in the return to estrus rate (3.6%; 4.3%, pregnancy rate at 21 days post-insemination (99.5%; 97.2%, adjusted farrowing rate (94.9%; 94.3% and average litter size (11.6; 11.8 piglets between treatments, respectively. The intrauterine insemination ensures a reproductive performance similar to traditional, but with a lower sperm number.

  10. Metabolites of MDMA induce oxidative stress and contractile dysfunction in adult rat left ventricular myocytes

    OpenAIRE

    Shenouda, Sylvia K.; Varner, Kurt J.; Carvalho, Felix; Lucchesi, Pamela A.

    2009-01-01

    Repeated administration of MDMA (ecstasy) produces eccentric left ventricular (LV) dilation and diastolic dysfunction. While the mechanism(s) underlying this toxicity are unknown; oxidative stress plays an important role. MDMA is metabolized into redox cycling metabolites that produce superoxide. In this study, we demonstrated that metabolites of MDMA induce oxidative stress and contractile dysfunction in adult rat left ventricular myocytes. Metabolites of MDMA used in this study included: al...

  11. Inhibitory effects of genistein and resveratrol on guinea pig gallbladder contractility in vitro

    Institute of Scientific and Technical Information of China (English)

    Long-De Wang; Xiao-Qing Qiu; Zhi-Feng Tian; Ying-Fu Zhang; Hong-Fang Li

    2008-01-01

    AIM:To observe and compare the effects of phytoestrogen genistein,resveratrol and 17β-estradiol on the tonic contraction and the phasic contraction of isolated gallbladder muscle strips and to study the underlying mechanisms.METHODS:Isolated strips of gallbladder muscle from guinea pigs were suspended in organ baths containing Kreb's solution,and the contractilities of strips were measured before and after incubation with genistein,resveratrol and 17β-estradiol respectively.RESULTS:Similar to 17β-estradiol,genistein and resveratrol could dose-dependently inhibit the phasic contractile activities,they decreased the mean contractlie amplitude and the contractlie frequencies of gallbladder muscle strips,and also produced a marked reduction in resting tone.The blocker of estrogen receptor ICI 182780 failed to alter the inhibitory effects induced by genistein and resveratrol,but potassium bisperoxo(1,10 phenanthroline)oxovanadate bpV(phen),a potent protein tyrosine phosphatase inhibitor,markedly attenuated the inhibitory effects induced by genistein and resveratrol.In calcium-free Kreb's solution containing 0.01 mmol/L egtazic acid(EGTA),genistein and resveratrol inhibited the first phasic contraction induced by acetylcholine(Ach),but did not affect the second contraction induced by CaCl2.In addition,genistein,resveratrol and 17β-estradiol also could reduce the contractile responses of Ach and KCI,and shift their cumulative concentration-response curves rightward.CONCLUSION:Phytoestrogen genistein and resveratrol can directly inhibit the contractile activity of isolated gallbladder muscle both at rest and in response to stimulation.The mechanisms responsible for the inhibitory effects probably due mainly to inhibition of tyrosine kinase,Ca2+ influx through potential-dependent calcium channels(PDCs)and Ca2+ release from sarcoplasmic reticulum(SR),but were not related to the estrogen receptors.

  12. Contractile properties of the pigeon supracoracoideus during different modes of flight

    OpenAIRE

    Tobalske, Brett W.; Biewener, Andrew Austin

    2008-01-01

    The supracoracoideus (SUPRA) is the primary upstroke muscle for avian flight and is the antagonist to the downstroke muscle, the pectoralis (PECT). We studied in vivo contractile properties and mechanical power output of both muscles during take-off, level and landing flight. We measured muscle length change and activation using sonomicrometry and electromyography, and muscle force development using strain recordings on the humerus. Our results support a hypothesis that the primary role of ...

  13. Testosterone regulates smooth muscle contractile pathways in the rat prostate: emphasis on PDE5 signaling

    OpenAIRE

    Zhang, Xinhua; Zang, Ning; Wei, Yu; Yin, Jin; Teng, Ruobing; Seftel, Allen; DiSanto, Michael E.

    2011-01-01

    Testosterone (T) plays a permissive role in the development of benign prostatic hyperplasia (BPH), and phosphodiesterase 5 inhibitors (PDE5is) have been found to be effective for BPH and lower urinary tract symptoms (LUTS) in clinical trials. This study investigated the effect of T on smooth muscle (SM) contractile and regulatory signaling pathways, including PDE5 expression and functional activity in prostate in male rats (sham-operated, surgically castrated, and castrated with T supplementa...

  14. The p90 ribosomal S6 kinase (RSK is a mediator of smooth muscle contractility.

    Directory of Open Access Journals (Sweden)

    Mykhaylo Artamonov

    Full Text Available In the canonical model of smooth muscle (SM contraction, the contractile force is generated by phosphorylation of the myosin regulatory light chain (RLC20 by the myosin light chain kinase (MLCK. Moreover, phosphorylation of the myosin targeting subunit (MYPT1 of the RLC20 phosphatase (MLCP by the RhoA-dependent ROCK kinase, inhibits the phosphatase activity and consequently inhibits dephosphorylation of RLC20 with concomitant increase in contractile force, at constant intracellular [Ca(2+]. This pathway is referred to as Ca(2+-sensitization. There is, however, emerging evidence suggesting that additional Ser/Thr kinases may contribute to the regulatory pathways in SM. Here, we report data implicating the p90 ribosomal S6 kinase (RSK in SM contractility. During both Ca(2+- and agonist (U46619 induced SM contraction, RSK inhibition by the highly selective compound BI-D1870 (which has no effect on MLCK or ROCK resulted in significant suppression of contractile force. Furthermore, phosphorylation levels of RLC20 and MYPT1 were both significantly decreased. Experiments involving the irreversible MLCP inhibitor microcystin-LR, in the absence of Ca(2+, revealed that the decrease in phosphorylation levels of RLC20 upon RSK inhibition are not due solely to the increase in the phosphatase activity, but reflect direct or indirect phosphorylation of RLC20 by RSK. Finally, we show that agonist (U46619 stimulation of SM leads to activation of extracellular signal-regulated kinases ERK1/2 and PDK1, consistent with a canonical activation cascade for RSK. Thus, we demonstrate a novel and important physiological function of the p90 ribosomal S6 kinase, which to date has been typically associated with the regulation of gene expression.

  15. Doppler-derived myocardial systolic strain rate is a strong index of left ventricular contractility

    Science.gov (United States)

    Greenberg, Neil L.; Firstenberg, Michael S.; Castro, Peter L.; Main, Michael; Travaglini, Agnese; Odabashian, Jill A.; Drinko, Jeanne K.; Rodriguez, L. Leonardo; Thomas, James D.; Garcia, Mario J.

    2002-01-01

    BACKGROUND: Myocardial fiber strain is directly related to left ventricular (LV) contractility. Strain rate can be estimated as the spatial derivative of velocities (dV/ds) obtained by tissue Doppler echocardiography (TDE). The purposes of the study were (1) to determine whether TDE-derived strain rate may be used as a noninvasive, quantitative index of contractility and (2) to compare the relative accuracy of systolic strain rate against TDE velocities alone. METHODS AND RESULTS: TDE color M-mode images of the interventricular septum were recorded from the apical 4-chamber view in 7 closed-chest anesthetized mongrel dogs during 5 different inotropic stages. Simultaneous LV volume and pressure were obtained with a combined conductance-high-fidelity pressure catheter. Peak elastance (Emax) was determined as the slope of end-systolic pressure-volume relationships during caval occlusion and was used as the gold standard of LV contractility. Peak systolic TDE myocardial velocities (Sm) and peak (epsilon'(p)) and mean (epsilon'(m)) strain rates obtained at the basal septum were compared against Emax by linear regression. Emax as well as TDE systolic indices increased during inotropic stimulation with dobutamine and decreased with the infusion of esmolol. A stronger association was found between Emax and epsilon'(p) (r=0.94, P<0.01, y=0.29x+0.46) and epsilon'(m) (r=0.88, P<0.01) than for Sm (r=0.75, P<0.01). CONCLUSIONS: TDE-derived epsilon'(p) and epsilon'(m) are strong noninvasive indices of LV contractility. These indices appear to be more reliable than S(m), perhaps by eliminating translational artifact.

  16. A computational study of stress fiber-focal adhesion dynamics governing cell contractility

    OpenAIRE

    Maraldi, Mirko; Valero, Clara; Garikipati, Krishna

    2014-01-01

    We apply a recently developed model of cytoskeletal force generation to study a cell intrinsic contractility, as well as its response to external loading. The model is based on a non-equilibrium thermodynamic treatment of the mechano-chemistry governing force in the stress fiber-focal adhesion system. Our computational study suggests that the mechanical coupling between the stress fibers and focal adhesions leads to a complex, dynamic, mechano-chemical response. We collect the results in resp...

  17. The contribution of Kv7 channels to pregnant mouse and human myometrial contractility

    OpenAIRE

    McCallum, Laura A; Pierce, Stephanie L.; ENGLAND, SARAH K.; Greenwood, Iain A.; Tribe, Rachel M.

    2010-01-01

    Abstract Premature birth accounts for approximately 75% of neonatal mortality and morbidity in the developed world. Despite this, methods for identifying and treating women at risk of preterm labour are limited and many women still present in preterm labour requiring tocolytic therapy to suppress uterine contractility. The aim of this study was to assess the utility of Kv7 channel activators as potential uterine smooth muscle (myometrium) relaxants in tissues from pregnant mice and women. Myo...

  18. Lymphatic filariasis: Perspectives on lymphatic remodeling and contractile dysfunction in filarial disease pathogenesis

    OpenAIRE

    Chakraborty, Sanjukta; Gurusamy, Manokaran; Zawieja, David C.; Muthuchamy, Mariappan

    2013-01-01

    Lymphatic filariasis, one of the most debilitating diseases associated with the lymphatic system, affects over a hundred million people worldwide and manifests itself in a variety of severe clinical pathologies. The filarial parasites specifically target the lymphatics and impair lymph flow, which is critical for the normal functions of the lymphatic system in maintenance of body fluid balance and physiological interstitial fluid transport. The resultant contractile dysfunction of the lymphat...

  19. Raloxifene acutely suppresses ventricular myocyte contractility through inhibition of the L-type calcium current

    OpenAIRE

    Liew, Reginald; Stagg, Mark A; MacLeod, Kenneth T; Collins, Peter

    2004-01-01

    The selective oestrogen (ER) receptor modulator, raloxifene, is widely used in the treatment of postmenopausal osteoporosis, but may also possess cardioprotective properties. We investigated whether it directly suppresses myocyte contractility through Ca2+ channel antagonism in a similar way to 17β-oestradiol.Cell shortening and Ca2+ transients were measured in single guinea-pig ventricular myocytes field-stimulated (1 Hz, 37°C) in a superfusion chamber. Electrophysiological recordings were p...

  20. Physiological and clinical aspects of uterine contractility during the postpartum period in cows

    OpenAIRE

    Bajcsy, Árpád Csaba

    2005-01-01

    The individual studies, presented in separate chapters of this thesis, were designed to get answers to certain methodological problems and biological questions associated with the myometrial function of early postpartum dairy cows. Chapter 1 introduces the topic by briefly summarizing the events occuring in the puerperal uterus, focusing on the role of myometrial contractions, and listing some of the possibilities used to record uterine contractility. The demand for an accurate, non-invasive,...

  1. Smooth muscle cells in bovine cervical ripening and dilatation; contractility, degrading enzymes and inflammation

    OpenAIRE

    Engelen, E.

    2008-01-01

    Cervical ripening is a complex process of modification of cervical tissue that enables dilation of the cervix at parturition. Cervical smooth muscle tissue might play a role by contracting or by secretion of cytokines or MMPs. To assess a contractile role for the cervical smooth muscle cells in cervical dilatation, we measured cervical diameter with ultrasound cervimetry together with EMG-activity of the myometrium, cervical outer muscular layer (COML) and cervical stromal layer (CSL). This w...

  2. AAV-mediated gene therapy for heart failure: enhancing contractility and calcium handling

    OpenAIRE

    Zouein, Fouad A.; Booz, George W.

    2013-01-01

    Heart failure is a progressive, debilitating disease that is characterized by inadequate contractility of the heart. With an aging population, the incidence and economic burden of managing heart failure are anticipated to increase substantially. Drugs for heart failure only slow its progression and offer no cure. However, results of recent clinical trials using recombinant adeno-associated virus (AAV) gene delivery offer the promise, for the first time, that heart failure can be reversed. The...

  3. Formation of mitochondrial apparatus of contractile cardiomyocytes during normal and hypoxic injury of cardi-ogenesis

    OpenAIRE

    Ivanchenko M.V.; Tverdokhlib I.V.

    2013-01-01

    Changes of cardiomyocytes mitochondrial apparatus can be marked as the main factors which are the basis of various forms of cardiovascular disease, but the dynamics of morphogenetic rearrangements heart mitochondria are poorly researched under normal conditions and under the influence of harmful factors. Mitochondria of contractile cardiomyocytes are different in their morphology and localization in the cell, the biochemical properties and are able to form differently association with other i...

  4. Impaired contractility and remodeling of the upper gastrointestinal tract in diabetes mellitus type-1

    Institute of Scientific and Technical Information of China (English)

    Jens Brφndum Frφkjaer; Sφren Due Andersen; Niels Ejskjaer; Peter Funch-Jensen; Asbjφrn Mohr Drewes; Hans Gregersen

    2007-01-01

    AIM: To investigate that both the neuronal function of the contractile system and structural apparatus of the gastrointestinal tract are affected in patients with longstanding diabetes and auto mic neuropathy.METHODS: The evoked esophageal and duodenal contractile activity to standardized bag distension was assessed using a specialized ultrasound-based probe. Twelve type-1 diabetic patients with autonomic neuropathy and severe gastrointestinal symptoms and 12 healthy controls were studied. The geometry and biomechanical parameters (strain, tension/stress, and stiffness) were assessed.RESULTS: The diabetic patients had increased frequency of distension-induced contractions (6.0 ±0.6 vs 3.3 ± 0.5, P < 0.001). This increased reactivity was correlated with the duration of the disease (P =0.009). Impaired coordination of the contractile activity in diabetic patients was demonstrated as imbalance between the time required to evoke the first contraction at the distension site and proximal to it (1.5 ± 0.6 vs 0.5± 0.1, P = 0.03). The esophageal wall and especially the mucosa-submucosa layer had increased thickness in the patients (P < 0.001), and the longitudinal and radial compressive stretch was less in diabetics (P <0.001). The esophageal and duodenal wall stiffness and circumferential deformation induced by the distensions were not affected in the patients (all P > 0.14).CONCLUSION: The impaired contractile activity with an imbalance in the distension-induced contractions likely reflects neuronal abnormalities due to autonomic neuropathy. However, structural changes and remodeling of the gastrointestinal tract are also evident and may add to the neuronal changes. This may contribute to the pathophysiology of diabetic gut dysfunction and impact on future management of diabetic patients with gastrointestinal symptoms.

  5. Contractile and Elastic Ankle Joint Muscular Properties in Young and Older Adults

    OpenAIRE

    Hasson, Christopher J.; Miller, Ross H; Caldwell, Graham E.

    2011-01-01

    The purpose of this study was to investigate age-related differences in contractile and elastic properties of both dorsi- (DF) and plantarflexor (PF) muscles controlling the ankle joint in young and older adults. Experimental data were collected while twelve young and twelve older male and female participants performed maximal effort isometric and isovelocity contractions on a dynamometer. Equations were fit to the data to give torque-angle (Tθ) and torque-angular velocity (Tω) relations. Mus...

  6. Vascular smooth muscle cell-derived adiponectin: a paracrine regulator of contractile phenotype

    OpenAIRE

    Ding, Min; Carrao, Ana Catarina; Wagner, Robert J.; Xie, Yi; Jin, Yu; Rzucidlo, Eva M.; Yu, Jun; Li, Wei; Tellides, George; Hwa, John; Aprahamian, Tamar R.; Martin, Kathleen A.

    2011-01-01

    Adiponectin is a cardioprotective adipokine derived predominantly from visceral fat. We recently demonstrated that exogenous adiponectin induces vascular smooth muscle cell (VSMC) differentiation via repression of mTORC1 and FoxO4. Here we report for the first time that VSMC express and secrete adiponectin, which acts in an autocrine and paracrine manner to regulate VSMC contractile phenotype. Adiponectin was found to be expressed in human coronary artery and mouse aortic VSMC. Importantly, s...

  7. Arterial dP/dtmax accurately reflects left ventricular contractility during shock when adequate vascular filling is achieved

    OpenAIRE

    Morimont Philippe; Lambermont Bernard; Desaive Thomas; Janssen Nathalie; Chase Geoffrey; D'Orio Vincent

    2012-01-01

    Background: Peak first derivative of femoral artery pressure (arterial dP/dt max) derived from fluid-filled catheter remains questionable to assess left ventricular (LV) contractility during shock. The aim of this study was to test if arterial dP/dt maxis reliable for assessing LV contractility during various hemodynamic conditions such as endotoxin-induced shock and catecholamine infusion.Methods: Ventricular pressure-volume data obtain...

  8. Recent Insights in the Paracrine Modulation of Cardiomyocyte Contractility by Cardiac Endothelial Cells

    Directory of Open Access Journals (Sweden)

    Jacques Noireaud

    2014-01-01

    Full Text Available The cardiac endothelium is formed by a continuous monolayer of cells that line the cavity of the heart (endocardial endothelial cells (EECs and the luminal surface of the myocardial blood vessels (intramyocardial capillary endothelial cells (IMCEs. EECs and IMCEs can exercise substantial control over the contractility of cardiomyocytes by releasing various factors such as nitric oxide (NO via a constitutive endothelial NO-synthase (eNOS, endothelin-1, prostaglandins, angiotensin II, peptide growth factors, and neuregulin-1. The purpose of the present paper is actually to shortly review recent new information concerning cardiomyocytes as effectors of endothelium paracrine signaling, focusing particularly on contractile function. The modes of action and the regulatory paracrine role of the main mediators delivered by cardiac endothelial cells upon cardiac contractility identified in cardiomyocytes are complex and not fully described. Thus, careful evaluation of new therapeutic approaches is required targeting important physiological signaling pathways, some of which have been until recently considered as deleterious, like reactive oxygen species. Future works in the field of cardiac endothelial cells and cardiac function will help to better understand the implication of these mediators in cardiac physiopathology.

  9. Acute pergolide exposure stiffens engineered valve interstitial cell tissues and reduces contractility in vitro.

    Science.gov (United States)

    Capulli, Andrew K; MacQueen, Luke A; O'Connor, Blakely B; Dauth, Stephanie; Parker, Kevin Kit

    2016-01-01

    Medications based on ergoline-derived dopamine and serotonin agonists are associated with off-target toxicities that include valvular heart disease (VHD). Reports of drug-induced VHD resulted in the withdrawal of appetite suppressants containing fenfluramine and phentermine from the US market in 1997 and pergolide, a Parkinson's disease medication, in 2007. Recent evidence suggests that serotonin receptor activity affected by these medications modulates cardiac valve interstitial cell activation and subsequent valvular remodeling, which can lead to cardiac valve fibrosis and dysfunction similar to that seen in carcinoid heart disease. Failure to identify these risks prior to market and continued use of similar drugs reaffirm the need to improve preclinical evaluation of drug-induced VHD. Here, we present two complimentary assays to measure stiffness and contractile stresses generated by engineered valvular tissues in vitro. As a case study, we measured the effects of acute (24 h) pergolide exposure to engineered porcine aortic valve interstitial cell (AVIC) tissues. Pergolide exposure led to increased tissue stiffness, but it decreased both basal and active contractile tone stresses generated by AVIC tissues. Pergolide exposure also disrupted AVIC tissue organization (i.e., tissue anisotropy), suggesting that the mechanical properties and contractile functionality of these tissues are governed by their ability to maintain their structure. We expect further use of these assays to identify off-target drug effects that alter the phenotypic balance of AVICs, disrupt their ability to maintain mechanical homeostasis, and lead to VHD. PMID:27174867

  10. [Contractile reaction of the myocardium of patients with heart diseases to chemical scarification of cell membranes].

    Science.gov (United States)

    Shumakov, V I; Tsyv'ian, P B; Markhasin, V S; Shtengol'd, E Sh

    1978-03-01

    Strips of the myocardium from the auricula atria of patients suffering from mitral stenosis (MS) and septal defects of the heart (SDH) removed during the operation were treated with ethylenediaminetetraacetic acid (DETA)--3mM--to increase the cell membrane permeability (scarification). The mechanical response of the contractile proteins to the change in the Ca2+ was recorded in the ethylene-hexaaminetetraacetic acid (EHTA)--3mM--against the background of increased membrane permeability to the Ca-EHTA complex permitting to regulate Ca2+ concentration in myofibrillae from 10(-9) to 10(-4)M. As shown, with the same threshold concentrations (5.10(-8)M) and saturation concentrations (10(-4)M) of Ca2+ the strips from the patients with MS developed the maximal tension per cross section unit of the strip half as great as the preparations from patients with SDH, this indicating a possible affection of the contractile proteins in the hearts of patients with MS. The ratio between the tension amplitudes under conditions of a complete calcium activation of the contractile proteins and a single isometric contraction for the preparations obtained from the patients with MS was 8 to 10, and with SDH--from 4 to 5. It is supposed that this was the result of more pronounced changes in the apparatus of electromechanical conjugation of the myocardium of patients suffering from MS. PMID:96886

  11. Proteome dynamics during contractile and metabolic differentiation of bovine foetal muscle.

    Science.gov (United States)

    Chaze, T; Meunier, B; Chambon, C; Jurie, C; Picard, B

    2009-07-01

    Contractile and metabolic properties of bovine muscles play an important role in meat sensorial quality, particularly tenderness. Earlier studies based on Myosin heavy chain isoforms analyses and measurements of glycolytic and oxidative enzyme activities have demonstrated that the third trimester of foetal life in bovine is characterized by contractile and metabolic differentiation. In order to complete this data and to obtain a precise view of this phase and its regulation, we performed a proteomic analysis of Semitendinosus muscle from Charolais foetuses analysed at three stages of the third trimester of gestation (180, 210 and 260 days). The results complete the knowledge of important changes in the profiles of proteins from metabolic and contractile pathways. They provide new insights about proteins such as Aldehyde dehydrogenase family, Enolase, Dihydrolipoyl dehydrogenase, Troponin T or Myosin light chains isoforms. These data have agronomical applications not only for the management of beef sensorial quality but also in medical context, as bovine myogenesis appears very similar to human one. PMID:22444818

  12. Improvement of diaphragm and limb muscle isotonic contractile performance by K+ channel blockade

    Directory of Open Access Journals (Sweden)

    Pollarine Jennifer

    2010-01-01

    Full Text Available Abstract The K+ channel blocking aminopyridines greatly improve skeletal muscle isometric contractile performance during low to intermediate stimulation frequencies, making them potentially useful as inotropic agents for functional neuromuscular stimulation applications. Most restorative applications involve muscle shortening; however, previous studies on the effects of aminopyridines have involved muscle being held at constant length. Isotonic contractions differ substantially from isometric contractions at a cellular level with regards to factors such as cross-bridge formation and energetic requirements. The present study tested effects of 3,4-diaminopyridine (DAP on isotonic contractile performance of diaphragm, extensor digitorum longus (EDL and soleus muscles from rats. During contractions elicited during 20 Hz stimulation, DAP improved work over a range of loads for all three muscles. In contrast, peak power was augmented for the diaphragm and EDL but not the soleus. Maintenance of increased work and peak power was tested during repetitive fatigue-inducing stimulation using a single load of 40% and a stimulation frequency of 20 Hz. Work and peak power of both diaphragm and EDL were augmented by DAP for considerable periods of time, whereas that of soleus muscle was not affected significantly. These results demonstrate that DAP greatly improves both work and peak power of the diaphragm and EDL muscle during isotonic contractions, which combined with previous data on isometric contractions indicates that this agent is suitable for enhancing muscle performance during a range of contractile modalities.

  13. Contractile function is unaltered in diaphragm from mice lacking calcium release channel isoform 3

    Science.gov (United States)

    Clancy, J. S.; Takeshima, H.; Hamilton, S. L.; Reid, M. B.

    1999-01-01

    Skeletal muscle expresses at least two isoforms of the calcium release channel in the sarcoplasmic reticulum (RyR1 and RyR3). Whereas the function of RyR1 is well defined, the physiological significance of RyR3 is unclear. Some authors have suggested that RyR3 participates in excitation-contraction coupling and that RyR3 may specifically confer resistance to fatigue. To test this hypothesis, we measured contractile function of diaphragm strips from adult RyR3-deficient mice (exon 2-targeted mutation) and their heterozygous and wild-type littermates. In unfatigued diaphragm, there were no differences in isometric contractile properties (twitch characteristics, force-frequency relationships, maximal force) among the three groups. Our fatigue protocol (30 Hz, 0.25 duty cycle, 37 degrees C) depressed force to 25% of the initial force; however, lack of RyR3 did not accelerate the decline in force production. The force-frequency relationship was shifted to higher frequencies and was depressed in fatigued diaphragm; lack of RyR3 did not exaggerate these changes. We therefore provide evidence that RyR3 deficiency does not alter contractile function of adult muscle before, during, or after fatigue.

  14. Apelin administration ameliorates high fat diet-induced cardiac hypertrophy and contractile dysfunction.

    Science.gov (United States)

    Ceylan-Isik, Asli F; Kandadi, Machender R; Xu, Xihui; Hua, Yinan; Chicco, Adam J; Ren, Jun; Nair, Sreejayan

    2013-10-01

    Apelin has been recognized as an adipokine that plays an important role in regulating energy metabolism and is credited with antiobesity and antidiabetic properties. This study was designed to examine the effect of exogenous apelin on obesity-associated cardiac dysfunction. Oral glucose tolerance test, echocardiography, cardiomyocyte contractile and intracellular Ca(2+) properties were assessed in adult C57BL/6J mice fed - low or a - high-fat diet for 24weeks followed by apelin treatment (100nmol/kg, i.p. for 2weeks). High-fat diet resulted in increased left ventricular diastolic and systolic diameters, and wall thickness, compromised fractional shortening, impaired cardiomyocyte mechanics (peak-shortening, maximal velocity of shortening/relengthening, and duration of shortening and relengthening) and compromised intracellular Ca(2+) handling, all of which were reconciled by apelin. Apelin treatment also reversed high fat diet-induced changes in intracellular Ca(2+) regulatory proteins, ER stress, and autophagy. In addition, microRNAs (miR) -133a, miR-208 and miR-1 which were elevated following high-fat feeding were attenuated by apelin treatment. In cultured cardiomyocytes apelin reconciled palmitic acid-induced cardiomyocyte contractile anomalies. Collectively, these data depict a pivotal role of apelin in obesity-associated cardiac contractile dysfunction, suggesting a therapeutic potential of apelin in the management of cardiac dysfunction associated with obesity. PMID:23859766

  15. Local 3D matrix microenvironment regulates cell migration through spatiotemporal dynamics of contractility-dependent adhesions

    Science.gov (United States)

    Doyle, Andrew D.; Carvajal, Nicole; Jin, Albert; Matsumoto, Kazue; Yamada, Kenneth M.

    2015-11-01

    The physical properties of two-dimensional (2D) extracellular matrices (ECMs) modulate cell adhesion dynamics and motility, but little is known about the roles of local microenvironmental differences in three-dimensional (3D) ECMs. Here we generate 3D collagen gels of varying matrix microarchitectures to characterize their regulation of 3D adhesion dynamics and cell migration. ECMs containing bundled fibrils demonstrate enhanced local adhesion-scale stiffness and increased adhesion stability through balanced ECM/adhesion coupling, whereas highly pliable reticular matrices promote adhesion retraction. 3D adhesion dynamics are locally regulated by ECM rigidity together with integrin/ECM association and myosin II contractility. Unlike 2D migration, abrogating contractility stalls 3D migration regardless of ECM pore size. We find force is not required for clustering of activated integrins on 3D native collagen fibrils. We propose that efficient 3D migration requires local balancing of contractility with ECM stiffness to stabilize adhesions, which facilitates the detachment of activated integrins from ECM fibrils.

  16. Contractile roots in succulent monocots: convergence, divergence and adaptation to limited rainfall.

    Science.gov (United States)

    North, Gretchen B; Brinton, Erin K; Garrett, Tadao Y

    2008-08-01

    Contractile roots (CRs) that pull shoots further down in the soil are a possible example of convergent evolution in two monocot families, the Agavaceae and the Asphodelaceae. The association between CRs, water uptake and habitat aridity was investigated for agaves, yuccas and aloes by assessing the occurrence of CRs and the amount of root contraction for glasshouse-grown plants with respect to mean annual rainfall of their native habitats. Structural features of CRs as well as root hydraulic conductance were compared with those of non-contractile roots (NCRs). CRs occurred in 55% of the 73 species examined, including 64% of the agaves and 85% of the yuccas, but in none of the aloes despite the occurrence of CRs in related genera. The phylogenetic distribution of CRs was consistent with multiple acquisitions or losses of the trait. The amount of root contraction showed a highly significant negative relationship with mean annual rainfall, although other environmental factors may also be important. Radial hydraulic conductance of the basal (contractile) zone exceeded that of the midroot zone for CRs; for NCRs, the opposite was true. Thus, CRs in the species examined may provide a mechanism for greater water uptake near the soil surface in regions with limited rainfall. PMID:18507804

  17. A functional connection of Dictyostelium paracaspase with the contractile vacuole and a possible partner of the vacuolar proton ATPase

    Indian Academy of Sciences (India)

    Entsar Saheb; Ithay Biton; Katherine Maringer; John Bush

    2013-09-01

    Dictyostelium discoideum possesses only one caspase family member, paracaspase (pcp). Two separate mutant cell lines were first analysed: one cell line was an over-expressed GFP-tagged Pcp (GFP-Pcp), while the other cell line was a pcp-null (pcp-). Microscopic analysis of cells expressing GFP-Pcp revealed that Pcp was associated with the contractile vacuole membrane consisting of bladder-like vacuoles. This association was disrupted when cells were exposed to osmotic stress conditions. Compared with wild-type cells, the GFP-Pcp-over-expressing cells were susceptible to osmotic stress and were seen to be very rounded in hypo-osmotic conditions and contained more abnormally swollen contractile vacuole. Cells with pcp- were also rounded but had few, if any, contractile vacuoles. These observations suggest that Pcp is essential for Dictyostelium osmotic regulation via its functioning in the contractile vacuole system. Subjecting these cells to selected contractile vacuole inhibitor provided additional support for these findings. Furthermore, yeast two-hybrid system identified vacuolar proton ATPase (VatM) as the protein interacting with Pcp. Taken together, this work gives evidence for an eukaryotic paracaspase to be associated with both localization in and regulation of the contractile vacuolar system, an organelle critical for maintaining the normal morphology of the cell.

  18. Capacidad de carga estática en rodamientos. Normalización y tendencias.

    Directory of Open Access Journals (Sweden)

    A. García Toll

    2007-01-01

    Full Text Available Para ilustrar la funcionalidad de la norma NC-ISO 76: 2006, establecida a partir de una adopción idéntica de la Norma ISO 76: 1987/ amd.1: 1999 (E “Cojinetes de Rodamiento – Capacidad de Carga Estática”, son mostradas las correspondencias entre las magnitudes de capacidad de carga estática declaradas en los catálogos técnicos de reconocidos fabricantes de rodamientos con los valores presentes en NC-ISO 76 y considerando diferentes geometrías de rodamientos radiales de bolas rígidos y de contacto angular, rodamientos axiales de bolas y rodamientos de rodillos cilíndricos. Adicionalmente, el estudio ha permitido establecer las dependencias entre la capacidad de carga nominal estática del rodamiento y algunos parámetros geométricos, como el diámetro del elemento rodante, el diámetro interior y la relación diámetro interior - diámetro exterior del cojinete.At the present time, Standard NC-ISO 76: 2006 is a national standard established as identical adoption of ISO standard 76:1987 / amd.1: 1999 (E "Rolling Bearings - Static Load Rating¨. In this sense, the static load rating of rolling bearings declared in technical catalogs of renowned bearing manufacturers and the basic static load rating according to calculation procedures stated in Standard NC-ISO 76 are compared. The evaluation has taken into account different geometries of radial groove ball bearings, angular contact ball bearings, thrust ball bearings and radial roller bearings. Additionally, they are established dependences between the static load rating of rolling bearings and the interior diameter of different types of radial and thrust bearings.

  19. Recursos y Capacidades de Tecnología y Desempeño Organizacional

    Directory of Open Access Journals (Sweden)

    Carmen Berenice Ynzunza Cortés

    2013-01-01

    Full Text Available La evolución tecnológica ha modificado a las organizaciones en todos los ámbitos laborales, poniendo en riesgo la ventaja competitiva y supervivencia de muchas orga nizaciones. Así, este estudio busca explorar los recursos y capacidades de tecnología e innovación y las tecnologías de la información y su relación con el desempeño organizacional , con el fin de encontrar estrategias que contribuyan al fortalecimiento de las PYMES. La población objeto de estudio fueron 646 empresas del sector industrial del Estado de Querétaro. El tamaño de la muestra alcanzado fue de 116 con un muestreo no probabilístico de conveniencia. La recolección de datos se hizo a través de entrevistas y la técnica estadística utilizada fue el análisis estructural con el software AMOS. Los resultados prueban la relación entre los recursos y capacidades de tecnología e innovación con el desempeño organizacional , y su vínculo con las tecnologías de la información , no así de las tecnologías de la información con el desempeño organizacional . Se concluye que los recursos y capacidades de tecnología son un factor clave para un crecimiento sostenido y un desempeño organizacional superior; inciden en todos los procesos productivos, empresariales y de gestión relacionados con la productividad, competitividad organizacional y por supuesto la ventaja competi tiva.

  20. Una aproximación al factor humano desde el enfoque de recursos y capacidad

    OpenAIRE

    Sánchez Pérez, José

    2010-01-01

    Este trabajo estudia las posibilidades de "recursos humanos" de tornarse un activo estratégico en el marco teórico de la visión de la empresa basada en recursos y capacidades. El propósito de este artículo es doble. Primero valorar si recursos humanos tiene consideración estratégica dentro de este enfoque, es decir, si supera ciertas condiciones de escasez, valía, heterogeneidad, dificultad para la imitación, etc. Y segundo, facilitar una nueva vía para la valoración del impacto organizativo ...

  1. Volumen y capacidad: de las unidades de medida antropométricas a las estandarizadas

    OpenAIRE

    Cruz, Angie Carolina; Vargas, Ángel Ricardo; Montes, Yeimy Esperanza

    2012-01-01

    El presente artículo evidencia una experiencia de aula relacionada con la caracterización y comprensión de las unidades de medida antropométricas y estandarizadas para la enseñanza de las magnitudes de volumen y capacidad a estudiantes de quinto grado a través de la resolución de problemas como metodología de clase. El trabajo desarrollado tuvo en cuenta diversos contextos y situaciones cotidianas que promovieron un aprendizaje significativo en los estudiantes sobre el sistema métrico, potenc...

  2. La educación científica intercultural y el enfoque de las capacidades

    OpenAIRE

    Liliana Valladares

    2010-01-01

    En este manuscrito se presentan datos del desarrollo humano de los pueblos indígenas en México que reflejan la exclusión social que viven, particularmente en los ámbitos de la educación y de la educación científica. Se propone la conformación de una educación científica intercultural como estrategia para la expansión de las capacidades de las personas para hacer y ser aquello que tienen razones para valorar. La educación científica intercultural se fundamenta en tres condiciones: 1) una persp...

  3. Capacidad de evacuación y suministro nodal en un sistema eléctrico

    OpenAIRE

    Caño Rojo, Rubén del

    2009-01-01

    El objetivo fundamental del presente proyecto consiste en determinar la capacidad de evacuación y suministro de los diferentes nudos de un sistema eléctrico aplicando la metodología adecuada. Se conseguirá conocer la cantidad de energía que se puede entregar u obtener de los diferentes nudos de una red eléctrica cumpliendo los requisitos de seguridad necesarios establecidos en los procedimientos de operación del sistema eléctrico español. Esto nos permitiría conocer la capacida...

  4. Consideraciones para el desarrollo de capacidades de investigación en universidades privadas

    OpenAIRE

    Maria Luisa Isla; Juan Carlos Seijo; Francisco Javier Espinosa

    2013-01-01

    Se presentan reflexiones y estrategias que contribuyan a formar capacidades de investigación en universidades privadas con investigadores jóvenes comprometidos con el avance del conocimiento en su área de investigación así como con su entorno natural y soc ial. Mediante una combinación de formación en el aula y formación experiencial, se presentan las habilidades investigativas a desarrollar y los ejes de formación universitaria que contribuyen a formar investigadores. Adicionalmente a esta f...

  5. De pesos y capacidades : una experiencia en La Bastida de les Alcusses (Moixent, Valencia)

    OpenAIRE

    Bonet Rosado, Helena; Izquierdo Peraile, María Isabel; Mata Parreño, Consuelo; Vives-Ferrándiz Sánchez, Jaime

    2007-01-01

    Los trabajos sobre despensa y almacenamiento realizados en varios yacimientos ibéricos del área valenciana están permitiendo calcular las capacidades de almacenamiento doméstico, como en la vivienda 2 de Los Villares de Caudete de las Fuentes, o la cantidad de cereal almacenado en un pequeño recinto fortificado, como el Puntal dels Llops de Olocau, siempre en función del número de ánforas, tinajas y demás recipientes de despensa documentados en el registro arqueológico. En esta misma l...

  6. CAPACIDAD DE PROPAGACIÓN Y CALIDAD DE PLANTA DE VARIEDADES MEXICANAS Y EXTRANJERAS DE FRESA

    OpenAIRE

    Geremías Rodríguez-Bautista; Guillermo Calderón-Zavala; David Jaen-Contreras; Arturo Curiel-Rodríguez

    2012-01-01

    El presente trabajo se realizó con la finalidad de evaluar comparativamente la capacidad de propagación y calidad de plantas de fresa en variedades mexicanas (CP-Jacona y CP-Zamorana) y extranjeras (Festival y Albion). Las cuatro variedades se establecieron en dos viveros localizados en Michoacán, México a diferente altitud: Tanaquillo (1,700 m) y Zirahuén (2,228 m). Se muestrearon ocho plantas madre y ocho plantas hijas como repeticiones. Las variables estudiadas fueron, número de coronas, n...

  7. Capacidade de Carga Turística: Um Estudo no Caminhos Rurais de Porto Alegre, RS

    Directory of Open Access Journals (Sweden)

    Juliane Magagnin Da Soller

    2013-10-01

    Full Text Available Nesse artigo, apresenta-se o contexto do turismo de base comunitária, além de breve histórico e discussão acerca do conceito e da metodologia da Capacidade de Carga Turística, assim como a adaptação da metodologia para estudo aqui relatado. Descreve-se o estudo realizado para determinar a Capacidade de Carga Turística, tendo por amostra as propriedades rurais partícipes da Associação Porto Alegre Rural (POA RURAL, mais especialmente do roteiro Caminhos Rurais de Porto Alegre, no estado do Rio Grande do Sul. Propôs-se adaptar a para o local a metodologia de cálculo de Capacidade de Carga Turística a partir de Cifuentes (1992; et al 1996, considerando suas particularidades, principalmente aquelas que dizem respeito a saúde e ao bem-estar das famílias que recebem os visitantes e aos impactos ambientais causados pelas construções de infraestrutura turística. A Capacidade de Carga Turística é uma metodologia desenvolvida para áreas preservadas de uso público. Assim, não costuma dar enfoque às questões referentes à comunidade local e aos resíduos gerados pelas instalações e pelos serviços prestados no Turismo.  Tourist Carrying Capacity: A study in the Caminhos Rurais de Porto Alegre, RS  - This paper presents the community based tourism and a brief history, concept and methodology of Tourist Carrying Capacity, and the adaptation of that methodology to the present case. The paper presents the study to determine the Tourist Carrying Capacity in rural proprieties that currently constitute the Porto Alegre Rural Association (RURAL POA, the tourist destination Caminhos Rurais de Porto Alegre, Rio Grande do Sul. The research proposes to adapt the methodology of Tourist Carrying Capacity from Cifuentes (1992, et al 1996, to the Caminhos Rurais, considering its particularities, especially those relate to health and well-being of families who receives visitors, and the environmental impacts caused by the construction of

  8. Personas con diabetes mellitus tipo 2 y su capacidad de agencia de autocuidado, cartagena

    OpenAIRE

    Herrera Lían, Arleth; Andrade, Yesid; Hernández, Orlando; Manrique, Julián; Faria, Karen; Machado, Mayerly

    2012-01-01

    Contexto: La diabetes mellitus tipo 2 se constituye en un problema de salud pública por las repercusiones bio-psicosociales, la presencia de complicaciones neurovasculares y metabólicas en la persona que la padece. Enfermería cumple un papel importante a través de la educación y la capacitación.Objetivo: Identificar la capacidad de agencia de autocuidado de las personas con diagnóstico de diabetes mellitus tipo 2, inscritos en los programas de control de diabetes en 19 UPAS de Cartagena.Metod...

  9. Las Capacidades Dinámicas e Administración Estratégica

    OpenAIRE

    Vidigal Fernandes Martins; Vinícius Silva Pereira

    2011-01-01

    Este ensayo teórico se centra en las teorías que explican cómo las organizaciones a obtener y mantener una ventaja competitiva. Tres Paradigmas se resumen, el paradigma y el paradigma de conflicto estratégico de Porter, y las conversaciones para poner fin al paradigma de recursos basado en recursos. "Capacidad dinámica" se derivan de la fuerte relación entre las nociones generalizadas de eficacia con respecto a los cambios y las fórmulas genéricas e la ventaja competitiva sustentable.

  10. Las Capacidades Dinámicas e Administración Estratégica

    Directory of Open Access Journals (Sweden)

    Vidigal Fernandes Martins

    2011-09-01

    Full Text Available Este ensayo teórico se centra en las teorías que explican cómo las organizaciones a obtener y mantener una ventaja competitiva. Tres Paradigmas se resumen, el paradigma y el paradigma de conflicto estratégico de Porter, y las conversaciones para poner fin al paradigma de recursos basado en recursos. "Capacidad dinámica" se derivan de la fuerte relación entre las nociones generalizadas de eficacia con respecto a los cambios y las fórmulas genéricas e la ventaja competitiva sustentable.

  11. La capacidad y el bienestar subjetivo como dimensiones de estudio de la calidad de vida

    OpenAIRE

    Beatriz Yasuko Arita Watanabe

    2008-01-01

    La Calidad de vida es trabajada científicamente como un constructo muy amplio que abarca diversos significados pero en lo general apunta a la búsqueda de la explicación de la buena vida, aquella que se disfruta, que satisface al que la vive y que le produce estados de felicidad. De allí que sea la persona, desde su percepción, la que evalúe su propia vida, sus capacidades y, en el propio contexto, desarrolle mecanismos de búsqueda del bienestar personal. El Plan de Naciones Unidas para el Des...

  12. ESTUDIO DE ALUMNOS DE EDUCACIÓN SECUNDARIA OBLIGATORIA CON ALTAS CAPACIDADES INTELECTUALES

    OpenAIRE

    Anda-Moreno, Verónica

    2012-01-01

    El presente Trabajo de Fin de Máster realizado para la obtención del título del Máster en Formación de Profesorado de Educación Secundaria Obligatoria, Bachillerato, Formación Profesional y Enseñanza de Idiomas, Especialidad en Física y Química, de la Universidad Internacional de La Rioja trata del estudio de Alumnos con Altas Capacidades Intelectuales de la Educación Secundaria Obligatoria del sistema educativo español. En el entorno escolar, siempre se han presentado casos de niños...

  13. Herramientas TIC para la intervención educativa en estudiantes con altas capacidades

    OpenAIRE

    Tárraga Mínguez, Raúl; Sanz-Cervera, Pilar; Pastor Cerezuela, Gemma; Fernández Andrés, María Inmaculada

    2014-01-01

    Los estudiantes con altas capacidades procesan la información de manera distinta, ya que estos poseen una elevada creatividad e implicación en la tarea. Este hecho implica la necesidad de llevar acabo una intervención educativa específica. Algunos modelos teóricos han demostrado la efectividad del uso de las TICs en la intervención de este tipo de alumnado, por lo que en el presente artículo se incluyen diferentes herramientas TIC que pueden ser de gran utilidad para los docentes. Entre estas...

  14. Altas capacidades. Propuesta de intervención para el aprendizaje de la historia

    OpenAIRE

    Gárriz Forné, Javier

    2013-01-01

    El presente estudio de carácter teórico, es una aproximación al conocimiento de las necesidades educativas del alumnado con altas capacidades intelectuales y pretende desarrollar una propuesta educativa que responda a esas necesidades, desde las ciencias sociales, concretamente desde la historia. Partiendo de la legislación vigente y teniendo en cuenta el planteamiento de algunos autores que abordan esta problemática, primero, comenzaré perfilando las características de este alumnado; pos...

  15. Alumnado con altas capacidades intelectuales: Programa de intervención

    OpenAIRE

    Arranz Melado, Luis Alfonso

    2015-01-01

    En el presente trabajo se hace un breve resumen conceptual de la literatura científica sobre las altas capacidades intelectuales. Se hace referencia a conceptos, teorías y modelos. Luego se analiza algunas de las características del alumnado con necesidades educativas especiales. Seguidamente, se hace referencia a los procedimientos y técnicas de evaluación de los estudiantes con necesidades educativas especiales. Se hace mención de las diferentes modalidades de respuesta educativa aplica...

  16. Alumnos con altas capacidades intelectuales: Propuesta de Intervención

    OpenAIRE

    Cano-Aguirre, María Isabel

    2012-01-01

    En este trabajo se ha realizado una revisión bibliográfica acerca de las características de los alumnos de altas capacidades intelectuales, su funcionamiento cerebral y los proyectos que se aplican en la actualidad. En la segunda parte se ha realizado una investigación con un enfoque cualitativo en la cual se proporcionó un cuestionario a un grupo de profesionales que participan en proyectos dirigidos a este tipo de alumnado con el fin de detectar la situación actual en la que se encuentran. ...

  17. Género, adolescencia y altas capacidades : un acercamiento a la afectividad

    OpenAIRE

    González González, Leybiz

    2012-01-01

    El presente estudio fue realizado en un grupo de chicas y chicos adolescentes pertenecientes a la Asociación Onubense de Altas Capacidades ARETÉ. Sus propósitos consistieron en analizar el aspecto afectivo-relacional a través de una perspectiva de género, en la que fueron identificadas las vivencias y áreas de conflictos psicológicos, las preocupaciones, temores, satisfacciones e insatisfacciones presentes. Asimismo, se pretendió develar algunos elementos de la conformación de ...

  18. La evaluación psicopedagógica del alumnado con altas capacidades intelectuales

    OpenAIRE

    Moliner García, Odet; Comes Nolla, Gabriel; Luque, Antonio; Díaz Pareja, Elena María

    2008-01-01

    Tras un análisis de la normativa estatal y autonómica sobre la evaluación psicopedagógica del alumnado con altas capacidades intelectuales, se presentan los aspectos más relevantes que toda evaluación psicopedagógica debe contemplar para que resulte lo más eficaz posible, y permita ofrecer una respuesta educativa adecuada a dicho alumnado e identifique los recursos y apoyos que pueda precisar. Tales decisiones se concretan en el informe psicopedagógico y este artículo recoge...

  19. Alumnos con indicios de altas capacidades en Educación Infantil

    OpenAIRE

    López-Gómez, Sheila

    2012-01-01

    En las aulas se encuentran niños de altas capacidades que no han sido diagnosticados y por lo tanto no se está atendiendo a sus necesidades como se debiera. Mediante este trabajo de fin de grado se ha pretendido señalar las características principales que permiten al tutor del aula reconocer a estos alumnos y poderles derivar a los orientadores que señalarán las pautas necesarias para conseguir que el alumno se desarrolle en plenitud y se sienta integrado en el aula. Para ello se van a exp...

  20. Capacidades físicas en jugadores de fútbol formativo de un club profesional

    OpenAIRE

    Benítez Sillero, J. D.; Da Silva-Grigoletto, M. E.; Muñoz Herrera, E.; Morente Montero, A.; Guillén del Castillo, M.

    2015-01-01

    Las características físicas del fútbol son muy diversas, aunque se reconocen como muy importantes la capacidad de realizar esfuerzos de alta intensidad y la de retrasar la fatiga en la mayor medida posible en esfuerzos intermitentes. Son diversos los trabajos que analizan dichas características en el fútbol profesional, aunque son menores los que analizan las categorías de formación. En el presente estudio se analiza la evolución por categorías de pruebas de salto, velocidad, agilidad y re...

  1. Enfoque de recursos y capacidades dinámicas: planteamientos teóricos

    OpenAIRE

    Fuentelsaz Lamata, Lucio

    2015-01-01

    La consolidación del enfoque de recursos y capacidades dinámicas como marco teórico de referencia requiere la revisión crítica de sus fundamentos y la aplicación a distintas dimensiones estratégicas de la empresa. En esta conferencia revisamos en cuál de dichas dimensiones se están produciendo los mayores avances. Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

  2. Papel de las capacidades en la valoración tecnológica

    OpenAIRE

    Jiménez, Claudia Nelcy; Cristancho, Andrés Darío; Castellanos, Oscar Fernando

    2011-01-01

    Tradicionalmente la valoración tecnológica se ha abordado con el enfoque financiero aplicado en la gestión de proyectos, centrándose en la dimensión económica. Sin embargo, diversos autores han detectado falencias en esta aproximación, proponiendo incluir técnicas que permitan tomar en cuenta aspectos cualitativos e intangibles de la tecnología. Considerando la definición amplia de la tecnología que abarca las capacidades tecnológicas de los sistemas y aparatos productivos, el objetivo de est...

  3. Capacidad de agencia de autocuidado y factores de riesgo cardiovascular en adolescentes

    OpenAIRE

    Morales Aguilar, Rosa del Socorro

    2013-01-01

    Este estudio tuvo como objetivo determinar la relación entre la capacidad de agencia de autocuidado y los factores de riesgo cardiovascular en adolescentes de una Institución de Educación Superior en el Distrito de Barranquilla. Metodología: Estudio descriptivo, exploratorio, de corte transversal, con abordaje cuantitativo, basado en la teoría del déficit de autocuidado de Orem. La muestra fue de 133 adolescentes, seleccionados mediante muestreo aleatorio simple. Se aplicó la escala ASA, el c...

  4. Tendencias Actuales de la Capacidad del menor y su derecho a ser escuchado

    OpenAIRE

    Luisa María Delgado Vizcaíno; Dashenka Vázquez del Sol

    2013-01-01

    La protección del patrimonio de los niños y las niñas ha sido preocupación del Derecho de Familia a través de su historia, sin embargo, el tratamiento de esta protección ha evolucionado con el tiempo, junto a la propia evolución de la familia y las relaciones paterno filiales. El tratamiento que el Derecho de Daños realiza de este tema tiene múltiples aristas, desde temas tan novedosos como la capacidad progresiva hasta lograr una 'protección integral al menor'.

  5. La adquisición de capacidades transversales: una experiencia en asignaturas de Ciencias Sociales

    OpenAIRE

    Juan Vigaray, María Dolores de; González Gascón, Elena; Subiza Martínez, Begoña; Martínez Mora, Carmen; Vallés Amores, María Luisa; Posadas García, José Adolfo; López García, Juan José; Hernández Ricarte, Victoria; Peris Ferrando, Josep Enric; Cuevas Casaña, Joaquim

    2011-01-01

    En este trabajo analizamos, en el contexto de las asignaturas que componen la Red Docente “I+Do+i” (Investigación, Docencia, e Innovación), cómo diferentes materias de ciencias sociales (matemáticas, estadística, distribución comercial y economía de la salud), contribuyen a que el alumnado adquiera distintas capacidades transversales en su formación. Para la realización del estudio se ha elaborado una encuesta que los estudiantes han contestado de manera voluntaria y anónima. Adicionalmente, ...

  6. Myocardial contractility in the echo lab: molecular, cellular and pathophysiological basis

    Directory of Open Access Journals (Sweden)

    Bombardini Tonino

    2005-09-01

    Full Text Available Abstract In the standard accepted concept, contractility is the intrinsic ability of heart muscle to generate force and to shorten, independently of changes in the preload or afterload with fixed heart rates. At molecular level the crux of the contractile process lies in the changing concentrations of Ca2+ ions in the myocardial cytosol. Ca2+ ions enter through the calcium channel that opens in response to the wave of depolarization that travels along the sarcolemma. These Ca2+ ions "trigger" the release of more calcium from the sarcoplasmic reticulum (SR and thereby initiate a contraction-relaxation cycle. In the past, several attempts were made to transfer the pure physiological concept of contractility, expressed in the isolated myocardial fiber by the maximal velocity of contraction of unloaded muscle fiber (Vmax, to the in vivo beating heart. Suga and Sagawa achieved this aim by measuring pressure/volume loops in the intact heart: during a positive inotropic intervention, the pressure volume loop reflects a smaller end-systolic volume and a higher end-systolic pressure, so that the slope of the pressure volume relationship moves upward and to the left. The pressure volume relationship is the most reliable index for assessing myocardial contractility in the intact circulation and is almost insensitive to changes in preload and after load. This is widely used in animal studies and occasionally clinically. The limit of the pressure volume relationship is that it fails to take into account the frequency-dependent regulation of contractility: the frequency-dependent control of transmembrane Ca2+ entry via voltage-gated Ca2+ channels provides cardiac cells with a highly sophisticated short-term system for the regulation of intracellular Ca2+ homeostasis. An increased stimulation rate increases the force of contraction: the explanation is repetitive Ca2+ entry with each depolarization and, hence, an accumulation of cytosolic calcium. As the heart

  7. Autoestima dos cuidadores de doentes oncológicos com capacidade funcional reduzida

    Directory of Open Access Journals (Sweden)

    Natália Stanko Moreira

    2015-06-01

    Full Text Available Objetivo: Avaliar a autoestima de cuidadores de doentes oncológicos com capacidade funcional reduzida. Métodos: Trata-se de estudo do tipo transversal, realizado em um hospital público, de grande porte, especializado em oncologia. Utilizou-se uma amostra por conveniência composta por 90 cuidadores principais de doentes com câncer com capacidade funcional (Karnofsky entre 20 e 50. Foram coletados dados sóciodemográficos, sobre os encargos envolvidos no cuidar e a autoestima foi avaliada pela Escala para Medida do Sentimento de Autoestima de Dela Coleta. Os dados foram analisados pelo teste de Mann-Whitney e de Kurskall-Wallis, com nível de significância de 5%. Resultados: A maioria dos cuidadores eram mulheres, com companheiros, com baixa renda mensal, mantinham relações familiares com o doente e apresentavam média autoestima. A autoestima foi associada de forma estatisticamente significante apenas com a renda familiar (p = 0,002. Conclusão: A autoestima dos cuidadores de pacientes oncológicos é influenciada pelas condições de vida, especialmente as financeiras.

  8. LA SUBJETIVIDAD EN EL ENFOQUE DEL DESARROLLO: CALIDAD DE VIDA, BIENESTAR SUBJETIVO Y CAPACIDADES

    Directory of Open Access Journals (Sweden)

    José Sandoval Díaz

    2014-01-01

    Full Text Available En las últimas décadas la calidad de vida (CV y las valoraciones subjetivas del bienestar están adquiriendo protagonismo como indicadores de desarrollo. La incorporación teórica de estos elementos han sido canalizados en dos modelos: por un lado el mode lo de la psicología positiva con el concepto de bienestar subjetivo (BS, el que lo considera como elemento necesario y suficiente para dar cuenta del desarrollo, y por otro, el enfoque de capacidades, en la línea que ha venido trabajando el PNUD y su Índice de Desarrollo Humano. En este trabajo se exponen los argumentos esgrimidos por ambos modelos y se discute el papel que juegan dentro del estudio del desarrollo. Como principal conclusión, si bien se plantea la ventaja teórica y práctica del enfoque de las capacidades por sobre el modelo de bienestar subjetivo, sin embargo, se considera pertinente complementar el estudio del desarrollo humano con la dimensión subjetiva.

  9. Evaluación de capacidades para testificar en víctimas con discapacidad intelectual

    Directory of Open Access Journals (Sweden)

    María José Contreras

    2015-01-01

    Full Text Available Las habilidades para testificar de las víctimas con discapacidad intelectual (DI suelen ser consideradas de acuerdo con estereotipos más que con su realidad, teniendo en cuenta que se observa una gran heterogeneidad en este colectivo. Por ello es necesario un procedimiento que permita conocer específicamente las características cognitivas de cada víctima con DI con el fin último de adaptar los procedimientos policiales y judiciales a sus capacidades y evaluar adecuadamente la información que aportan en sus testimonios. Con este objetivo se desarrolló el protocolo de entrevista CAPALIST, que evalúa las capacidades cognitivas que intervendrían en la aportación de testimonio. En el presente trabajo se describe la herramienta y se aportan datos sobre su aplicación a cuatro víctimas reales de abuso sexual con DI. Los resultados muestran mínimas diferencias en los acuerdos interjueces. Se concluye que el CAPALIST podría ser de utilidad en la evaluación de las habilidades para testificar en víctimas con DI.

  10. T-tubule disease: Relationship between t-tubule organization and regional contractile performance in human dilated cardiomyopathy.

    Science.gov (United States)

    Crossman, David J; Young, Alistair A; Ruygrok, Peter N; Nason, Guy P; Baddelely, David; Soeller, Christian; Cannell, Mark B

    2015-07-01

    Evidence from animal models suggest that t-tubule changes may play an important role in the contractile deficit associated with heart failure. However samples are usually taken at random with no regard as to regional variability present in failing hearts which leads to uncertainty in the relationship between contractile performance and possible t-tubule derangement. Regional contraction in human hearts was measured by tagged cine MRI and model fitting. At transplant, failing hearts were biopsy sampled in identified regions and immunocytochemistry was used to label t-tubules and sarcomeric z-lines. Computer image analysis was used to assess 5 different unbiased measures of t-tubule structure/organization. In regions of failing hearts that showed good contractile performance, t-tubule organization was similar to that seen in normal hearts, with worsening structure correlating with the loss of regional contractile performance. Statistical analysis showed that t-tubule direction was most highly correlated with local contractile performance, followed by the amplitude of the sarcomeric peak in the Fourier transform of the t-tubule image. Other area based measures were less well correlated. We conclude that regional contractile performance in failing human hearts is strongly correlated with the local t-tubule organization. Cluster tree analysis with a functional definition of failing contraction strength allowed a pathological definition of 't-tubule disease'. The regional variability in contractile performance and cellular structure is a confounding issue for analysis of samples taken from failing human hearts, although this may be overcome with regional analysis by using tagged cMRI and biopsy mapping. PMID:25953258

  11. Menor de edad y capacidad de ejercicio: Reto del Derecho Familiar contemporáneo Menor de edad y capacidad de ejercicio: Reto del Derecho Familiar contemporáneo.

    Directory of Open Access Journals (Sweden)

    Jetzabel Mireya Montejo Rivero

    2012-04-01

    Full Text Available Change in family relations that took place at the opening of the 21st comporting new paradigms in conceptions of minor is already in progress. These reflections are referred to understanding child legal status capacity that takes into consideration its legal ability from the perspective of dynamic participation in concrete legal relations according to his progressive ability and secures the protection of his rights at the same time. The present article demonstrate the legal concepts of individual, personality and ability are interpreted the legal ability of child individuals is evaluated following that viewpoint. The study outlines the necessity of harmonizing the notion of child and ability for “child’s best interest” in contemporary family low.Los cambios en las relaciones familiares acaecidos en los albores de siglo XXI, comportan nuevos paradigmas en la concepción de la capacidad del menor. En estas reflexiones, se hace referencia a la enunciación de la capacidad de obrar del menor desde una dinámica participativa en las relaciones jurídicas civiles y familiares acorde a su capacidad progresiva y una más efectiva protección al ejercicio de sus derechos. El presente artículo, expone sucintamente valoraciones sobre el ejercicio de la capacidad del menor a partir de la fundamentación teórica de los conceptos jurídicos de persona, personalidad y capacidad, en aras de armonizar las nociones de menor de edad y capacidad de ejercicio desde el interés superior del niño en el Derecho Familiar contemporáneo.

  12. Postsynaptic nicotinic receptor desensitized by non-contractile Ca2+ mobilization via protein kinase-C activation at the mouse neuromuscular junction.

    OpenAIRE

    Kimura, I.; Dezaki, K.; Tsuneki, H.; Kimura, M

    1995-01-01

    1. Non-contractile Ca2+ mobilization (unaccompanied by muscle contraction) was initiated by nerve stimulation in the presence of neostigmine (more than 0.03 microM) at the endplate region of mouse diaphragm muscles. In the process of nicotinic receptor desensitization, the depressant effect of non-contractile Ca2+ on contractile Ca2+ mobilization was investigated by measurement of Ca(2+)-aequorin luminescence. 2. When the phrenic nerve was stimulated with paired pulses having intervals of 150...

  13. Capacidad tecnológica y desarrollo de capital intelectual en nuevas empresas de base tecnológica

    OpenAIRE

    Julio César Acosta-Prado; Eduardo Bueno Campos; Mónica Longo-Somoza

    2014-01-01

    Este artículo analiza la relación entre la capacidad tecnológica y el capital intelectual. La creación y explotación del conocimiento son la fuente fundamental de la capacidad tecnológica de las empresas y del capital intelectual, así como también de las ventajas competitivas. Aquí se argumenta que cuando se crean y explotan las capacidades tecnológicas, recurriendo a procesos de conocimiento, también se crea y explota el capital intelectual de la empresa. En el análisis empírico se aplicó el...

  14. La cultura tecnológica como base de las capacidades y el aprendizaje tecnológico

    Directory of Open Access Journals (Sweden)

    Álvaro Carvajal Villaplana

    2011-11-01

    Full Text Available En este trabajo se parte del supuesto de la existencia de una correlación positiva y robusta entre las capacidades tecnológicas y la cultura tecnológica, al fomentar ésta última, por medio del aprendizaje formal e informal, el surgimiento y el desenvolvimiento de las capacidades tecnológicas en una sociedad determinada. Aquí se analizan tres aproximaciones a la noción de cultura tecnológica: (a epistémico, (b descriptivo y (c prescriptivo. También, se reflexiona sobre un tipo particular de capacidades tecnológicas: las de innovación.

  15. Comparación de cuatro métodos de restauración del adn en muestras de plasma y láminas de citología cérvico-uterina como una herramienta para mejorar la calidad de la muestra

    OpenAIRE

    Marquez, Pilar caterin

    2011-01-01

    Objetivos: comparar cuatro métodos de restauración del adn en plasma y láminas cérvico-uterinas como una herramienta para mejorar la calidad de la muestra. Métodos: a 20 muestras de plasma sanguíneo y 20 muestras de láminas citológicas, se les realizó aislamiento de adn mediante kit comercial y fenol-cloroformo. A todas las muestras se les realizó un tratamiento pre-pcr con cuatro diferentes tipos de actividad de adn polimerasa: 1. Exonucleasa y endonucleasa 5’-3’. 2. Exonucleasa 5’-3’. 3. Kl...

  16. Pulmonary artery radiocardiography and rheography in the diagnosis of hemodynamic and contractile function impairments of the right ventricle in patients with obstructive bronchitis

    International Nuclear Information System (INIS)

    Radiocardiography and rheography of the pulmonary artery were used to examine impairments in hemodynamics and contractile function of the right ventricle in 40 patients with chronic obstructive bronchitis complicated with persistent hypertension. Right ventricular hemodynamic and contractile impairments were shown to be not equivalent with similar clinical and functional signs of pulmonary hypertension. This fact indicates that the use of special techiques is of practical value in the determination of right ventricular hemodynamics and myocardial contractility in patients with chronic obstructive bronchitis. Radiocardiography and rheography of the pulmonary artery are sufficiently reliable noninvasive techniques for examining the hemodynamics and contractile function of the right ventricular myocardium

  17. El enfoque de capacidad de Amartya Sen y sus limitaciones para la ciudadanía y la sociedad civil

    Directory of Open Access Journals (Sweden)

    Maria Rosario Carvajal Muñoz

    2014-01-01

    Full Text Available Este trabajo parte del concepto de “capacidad” de Sen, definido como un aspecto de la libertad que requiere de oportunidades sustantivas, para aproximarse al término de capacidad política. La capacidad política, por tanto, es un aspecto de la libertad que comprende oportunidades de participación política sustantiva. Esta aproximación a la capacidad política está confrontada con dos corrientes del pensamiento: el institucionalismo trascendental o contractualismo y el comparativista. Considerando estas dos corrientes de pensamiento, los conceptos institucionales de ciudadanía activa y sociedad civil, promovidos en las últimas décadas por gobiernos neoliberales, son examinados de forma crítica. Asimismo, se relaciona la capacidad política con modelos teóricos clásicos de sociedad civil. Esta relación entre la capacidad política con los conceptos de ciudadanía y sociedad civil ayudan a barajar la hipótesis de que se privilegia en política el consenso por mayoría (enfoque trascendental o contractualista, frente al consenso por discusión (enfoque comparativista o consecuencialista. La tradición contractualista dificultaría el reconocimiento del enfoque comparativista, fundamentado en la idea de equidad de Sen, y esencial para un gobierno por discusión, limitando así la capacidad política.

  18. Capacidade para o trabalho entre trabalhadores de higiene e limpeza de um hospital universitário público

    Directory of Open Access Journals (Sweden)

    Larissa Gutierrez da Silva

    2010-03-01

    Full Text Available A capacidade para o trabalho é a base do bem-estar para o ser humano e a sua manutenção envolve condições de saúde e trabalho adequadas. Com o objetivo de verificar a capacidade para o trabalho entre trabalhadores de higiene e limpeza,realizou-se uma pesquisa quantitativa, do tipo descritivo-exploratória, em um hospital universitário público do norte paranaense, de março a junho de 2008, por meio da aplicação de um questionário constituído por dados sócio-demográficos e ocupacionais, e pelo Índice de Capacidade para o Trabalho (ICT. Os dados foram analisados pelo programa Epi Info 2002. A população foi composta por 98 trabalhadores, sendo que 82,4% possuem mais de 41 anos e 96,9% são mulheres. Quanto à avaliação da capacidade para o trabalho, 45,9% dos entrevistados obtiveram um ICT bom, 23,5% ótimo, 22,4% moderado e 8,2% baixo, com escore mínimo de 16 e máximo de 49. As doenças músculo esqueléticas foram as mais referidas pelos entrevistados. Os fatores relacionados à capacidade para o trabalho são múltiplos, assim, as ações para a prevenção erecuperação desta capacidade devem ser discutidas e conciliadas entre empregados e empregadores, garantindo um espaço para contextualização e reflexão acerca do processo de trabalho.

  19. Opportunities to Target Specific Contractile Abnormalities with Smooth Muscle Protein Kinase Inhibitors

    Directory of Open Access Journals (Sweden)

    Annegret Ulke-Lemée

    2010-05-01

    Full Text Available Smooth muscle is a major component of most hollow organ systems (e.g., airways, vasculature, bladder and gut/gastrointestine; therefore, the coordinated regulation of contraction is a key property of smooth muscle. When smooth muscle functions normally, it contributes to general health and wellness, but its dysfunction is associated with morbidity and mortality. Rho-associated protein kinase (ROCK is central to calcium-independent, actomyosin-mediated contractile force generation in the vasculature, thereby playing a role in smooth muscle contraction, cell motility and adhesion. Recent evidence supports an important role for ROCK in the increased vasoconstriction and remodeling observed in various models of hypertension. This review will provide a commentary on the development of specific ROCK inhibitors and their clinical application. Fasudil will be discussed as an example of bench-to-bedside development of a clinical therapeutic that is used to treat conditions of vascular hypercontractility. Due to the wide spectrum of biological processes regulated by ROCK, many additional clinical indications might also benefit from ROCK inhibition. Apart from the importance of ROCK in smooth muscle contraction, a variety of other protein kinases are known to play similar roles in regulating contractile force. The zipper-interacting protein kinase (ZIPK and integrin-linked kinase (ILK are two well-described regulators of contraction. The relative contribution of each kinase to contraction depends on the muscle bed as well as hormonal and neuronal stimulation. Unfortunately, specific inhibitors for ZIPK and ILK are still in the development phase, but the success of fasudil suggests that inhibitors for these other kinases may also have valuable clinical applications. Notably, the directed inhibition of ZIPK with a pseudosubstrate molecule shows unexpected effects on the contractility of gastrointestinal smooth muscle.

  20. Paradoxical effects of ginkgolide B on cardiomyocyte contractile function in normal and high-glucose environments

    Institute of Scientific and Technical Information of China (English)

    Jihye KIM; Qun LI; Cindy X FANG; Jun REN

    2006-01-01

    Aim: Ginkgo biloba extract is a natural product used widely for cerebral and cardiovascular diseases. It is mainly composed of terpene lactones (ginkgolide A and B) and flavone glycosides (eg quercetin and kaempferol).To better understand the cardiac electromechanical action of Ginkgo biloba extract in normal and diabetic states, this study was designed to examine the effect of ginkgolide B on cardiomyocyte contractile function under normal and high-glucose environments. Methods: Isolated adult rat ventricular myocytes were cultured for 6 h in a serum-free medium containing either normal (NG;5.5 mmol/L) or high (HG;25.5 mmol/L) glucose with or without ginkgolide B (0.5-2.0μg/mL). Mechanical properties were evaluated using the IonOptix MyoCam system. Contractile properties analyzed included peak shortening (PS),maximal velocity of shortening/relengthening (+dl/dt),time-to-PS (TPS) and time-to-90% relengthening (TR90). Levels of essential Ca2+ regulatory proteins sarco(endo)plasmic reticulum Ca2+ -ATPase (SERCA2a),phospholamban (PLB) and Na+-Ca2+ exchanger (NCX) were assessed by Western blotting. Results: Ginkgolide B nullified HG-induced prolongation in TR90. However, ginkgolide B depressed PS.±dl/dt and shortened TPS in NG and HG cells. Ginkgolide B also prolonged TR90 in NG cells. Western blot analysis revealed that HG upregulated SERCA2a and downregulated PLB expression without affecting that of NCX. Ginkgolide B disrupted the NG-HG response pattern in SERCA2a and NCX without affecting that of PLB. Conclusion: Ginkgolide B affects cardiomyocyte contractile function under NG or HG environments in a paradoxical manner, which may be attributed to uneven action on Ca2+ regulatory proteins under NG and HG conditions.

  1. Poor spontaneous and oxytocin-stimulated contractility in human myometrium from postdates pregnancies.

    Directory of Open Access Journals (Sweden)

    Sarah Arrowsmith

    Full Text Available Prolongation of pregnancy i.e. going more than 10 days over the estimated due date, complicates up to 10% of all pregnancies and is associated with increased risk to both mother and fetus. Despite the obvious need for contractions of the uterus to end pregnancy, there have been no studies directly examining the role of uterine smooth muscle, myometrium, in the aetiology of prolonged pregnancy. This study tested the hypothesis that the intrinsic contractile characteristics of myometrium taken from women with prolonged pregnancy (>41 weeks and 3 days was reduced compared to those delivering at term (39-41 weeks. We recruited women undergoing Caesarean Section (CS delivery either pre-labour (n = 27 or in labour (n = 66 at term or postdates. The contractile ability of the postdates myometrium, whether spontaneous or elicited by oxytocin or high-K solution, was significantly reduced compared to term myometrium. These differences remained when adjusted for parity and other maternal characteristics. The findings remained significant when expressed per cross sectional area. Histological examination revealed no differences between the two groups. The contractile differences were however related to intracellular Ca transients suggesting an effect of [Ca] on reduced force production in the postdates group. In summary, myometrium from prolonged pregnancies contracts poorly in vitro even when stimulated with oxytocin and in active labour. Responses to high K(+ and measurements of Ca suggest that alterations in excitation contraction coupling, rather than any histological changes of the myometrium, may underlie the differences between term and postdates myometrium. We show that postdates pregnancy is associated with poor myometrial activity and suggest that this may contribute to increased myometrial quiescence and hence, prolonged gestation.

  2. Changes of mitochondria in the contractile cardiomyocytes during postnatal rat ontogenesis

    Directory of Open Access Journals (Sweden)

    Kozlov S.V.

    2014-12-01

    Full Text Available Background. CVDs are the number 1 cause of death globally: more people die annually from CVDs than from any other cause. An estimated 17.5 million people died from CVDs in 2012, representing 31% of all global deaths. Of these deaths, an estimated 7.4 million were due to coronary heart disease and 6.7 million were due to stroke. Over three quarters of CVD deaths take place in low- and middle-income countries. Objective. Ultrastructural analysis of mitochondria in the rat contractile cardiomyocytes during postnatal ontogenesis. Methods. As the object of the study were used neonatal rat hearts, on the 5th, 10th, 15th, 30th days of life and mature animals. Hearts were investigated by the transmission electron microscopy. Volume density and numerical density of mitochondria were estimated. The Paired Student’s t-test was applied. Results. Was conducted a comprehensive ultrastructural analysis of mitochondria contractile cardiomyocytes, which allowed us to determine changes in the qualitative and quantitative parameters of mitochondria during postnatal ontogenesis, and helps to explain the dynamics and the development of mitochondria heart muscles cells after birth. Conclusion. It was shown that from the 1st to the 5th day there was a significant increase in volume density of mitochondria, which was accompanied by the increasing complexity of the ultrastructural organization of organelles. Following 20th day of postnatal ontogenesis mitochondrial structure was approaching the definitive condition and on the 30th day was the same as the mature myocardium. Citation: Kozlov SV, Mayevsky AE, Mіshalov VD, Sulayeva ON. [Changes of mitochondria in the contractile cardiomyocytes during postnatal rat ontogenesis]. Morphologia. 2014;8(4:37-42. Russian.

  3. Isolation and maintenance-free culture of contractile myotubes from Manduca sexta embryos.

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    Amanda L Baryshyan

    Full Text Available Skeletal muscle tissue engineering has the potential to treat tissue loss and degenerative diseases. However, these systems are also applicable for a variety of devices where actuation is needed, such as microelectromechanical systems (MEMS and robotics. Most current efforts to generate muscle bioactuators are focused on using mammalian cells, which require exacting conditions for survival and function. In contrast, invertebrate cells are more environmentally robust, metabolically adaptable and relatively autonomous. Our hypothesis is that the use of invertebrate muscle cells will obviate many of the limitations encountered when mammalian cells are used for bioactuation. We focus on the tobacco hornworm, Manduca sexta, due to its easy availability, large size and well-characterized muscle contractile properties. Using isolated embryonic cells, we have developed culture conditions to grow and characterize contractile M. sexta muscles. The insect hormone 20-hydroxyecdysone was used to induce differentiation in the system, resulting in cells that stained positive for myosin, contract spontaneously for the duration of the culture, and do not require media changes over periods of more than a month. These cells proliferate under normal conditions, but the application of juvenile hormone induced further proliferation and inhibited differentiation. Cellular metabolism under normal and low glucose conditions was compared for C2C12 mouse and M. sexta myoblast cells. While differentiated C2C12 cells consumed glucose and produced lactate over one week as expected, M. sexta muscle did not consume significant glucose, and lactate production exceeded mammalian muscle production on a per cell basis. Contractile properties were evaluated using index of movement analysis, which demonstrated the potential of these cells to perform mechanical work. The ability of cultured M. sexta muscle to continuously function at ambient conditions without medium replenishment

  4. Assessment of Muscle Contractile Properties at Acute Moderate Altitude Through Tensiomyography.

    Science.gov (United States)

    Morales-Artacho, Antonio J; Padial, Paulino; Rodríguez-Matoso, Dario; Rodríguez-Ruiz, David; García-Ramos, Amador; García-Manso, Juan Manuel; Calderón, Carmen; Feriche, Belén

    2015-12-01

    Under hypoxia, alterations in muscle contractile properties and faster fatigue development have been reported. This study investigated the efficacy of tensiomyography (TMG) in assessing muscle contractile function at acute moderate altitude. Biceps femoris (BF) and vastus lateralis (VL) muscles of 18 athletes (age 20.1 ± 6.1 years; body mass 65.4 ± 13.9 kg; height 174.6 ± 9.5 cm) were assessed at sea level and moderate altitude using electrically evoked contractions on two consecutive days. Maximum radial displacement (Dm), time of contraction (Tc), reaction time (Td), sustained contraction time (Ts), and relaxation time (Tr) were recorded at 40, 60, 80, and 100 mA. At altitude, VL showed lower Dm values at 40 mA (p = 0.008; ES = -0.237). Biceps femoris showed Dm decrements in all electrical stimulations (p  0.61). In VL, Tc was longer at altitude at 40 (p = 0.031, ES = 0.56), and 100 mA (p = 0.03, ES = 0.51). Regarding Td, VL showed significant increases in all electrical intensities under hypoxia (p ≤ 0.03, ES ≥ 0.33). TMG appears effective at detecting slight changes in the muscle contractile properties at moderate altitude. Further research involving TMG along with other muscle function assessment methods is needed to provide additional insight into peripheral neuromuscular alterations at moderate altitude. PMID:26562625

  5. Myocardial mitochondrial and contractile function are preserved in mice lacking adiponectin.

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    Martin Braun

    Full Text Available Adiponectin deficiency leads to increased myocardial infarct size following ischemia reperfusion and to exaggerated cardiac hypertrophy following pressure overload, entities that are causally linked to mitochondrial dysfunction. In skeletal muscle, lack of adiponectin results in impaired mitochondrial function. Thus, it was our objective to investigate whether adiponectin deficiency impairs mitochondrial energetics in the heart. At 8 weeks of age, heart weight-to-body weight ratios were not different between adiponectin knockout (ADQ-/- mice and wildtypes (WT. In isolated working hearts, cardiac output, aortic developed pressure and cardiac power were preserved in ADQ-/- mice. Rates of fatty acid oxidation, glucose oxidation and glycolysis were unchanged between groups. While myocardial oxygen consumption was slightly reduced (-24% in ADQ-/- mice in isolated working hearts, rates of maximal ADP-stimulated mitochondrial oxygen consumption and ATP synthesis in saponin-permeabilized cardiac fibers were preserved in ADQ-/- mice with glutamate, pyruvate or palmitoyl-carnitine as a substrate. In addition, enzymatic activity of respiratory complexes I and II was unchanged between groups. Phosphorylation of AMP-activated protein kinase and SIRT1 activity were not decreased, expression and acetylation of PGC-1α were unchanged, and mitochondrial content of OXPHOS subunits was not decreased in ADQ-/- mice. Finally, increasing energy demands due to prolonged subcutaneous infusion of isoproterenol did not differentially affect cardiac contractility or mitochondrial function in ADQ-/- mice compared to WT. Thus, mitochondrial and contractile function are preserved in hearts of mice lacking adiponectin, suggesting that adiponectin may be expendable in the regulation of mitochondrial energetics and contractile function in the heart under non-pathological conditions.

  6. Acute exposure to lead increases myocardial contractility independent of hypertension development

    International Nuclear Information System (INIS)

    We studied the effects of the acute administration of small doses of lead over time on hemodynamic parameters in anesthetized rats to determine if myocardial contractility changes are dependent or not on the development of hypertension. Male Wistar rats received 320 µg/kg lead acetate iv once, and their hemodynamic parameters were measured for 2 h. Cardiac contractility was evaluated in vitro using left ventricular papillary muscles as were Na+,K+-ATPase and myosin Ca2+-ATPase activities. Lead increased left- (control: 112 ± 3.7 vs lead: 129 ± 3.2 mmHg) and right-ventricular systolic pressures (control: 28 ± 1.2 vs lead: 34 ± 1.2 mmHg) significantly without modifying heart rate. Papillary muscles were exposed to 8 µM lead acetate and evaluated 60 min later. Isometric contractions increased (control: 0.546 ± 0.07 vs lead: 0.608 ± 0.06 g/mg) and time to peak tension decreased (control: 268 ± 13 vs lead: 227 ± 5.58 ms), but relaxation time was unchanged. Post-pause potentiation was similar between groups (n = 6 per group), suggesting no change in sarcoplasmic reticulum activity, evaluated indirectly by this protocol. After 1-h exposure to lead acetate, the papillary muscles became hyperactive in response to a β-adrenergic agonist (10 µM isoproterenol). In addition, post-rest contractions decreased, suggesting a reduction in sarcolemmal calcium influx. The heart samples treated with 8 µM lead acetate presented increased Na+,K+-ATPase (approximately 140%, P < 0.05 for control vs lead) and myosin ATPase (approximately 30%, P < 0.05 for control vs lead) activity. Our results indicated that acute exposure to low lead concentrations produces direct positive inotropic and lusitropic effects on myocardial contractility and increases the right and left ventricular systolic pressure, thus potentially contributing to the early development of hypertension

  7. A single resistance exercise session improves myocardial contractility in spontaneously hypertensive rats

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    A.A. Fernandes

    2015-01-01

    Full Text Available Resistance training evokes myocardial adaptation; however, the effects of a single resistance exercise session on cardiac performance are poorly understood or investigated. This study aimed to investigate the effects of a single resistance exercise session on the myocardial contractility of spontaneously hypertensive rats (SHRs. Male 3-month-old SHRs were divided into two groups: control (Ct and exercise (Ex. Control animals were submitted to sham exercise. Blood pressure was measured in conscious rats before the exercise session to confirm the presence of arterial hypertension. Ten minutes after the exercise session, the animals were anesthetized and killed, and the hearts were removed. Cardiac contractility was evaluated in the whole heart by the Langendorff technique and by isometric contractions of isolated left ventricular papillary muscles. SERCA2a, phospholamban (PLB, and phosphorylated PLB expression were investigated by Western blot. Exercise increased force development of isolated papillary muscles (Ex=1.0±0.1 g/mg vs Ct=0.63±0.2 g/mg, P<0.05. Post-rest contraction was greater in the exercised animals (Ex=4.1±0.4% vs Ct=1.7±0.2%, P<0.05. Papillary muscles of exercised animals developed greater force under increasing isoproterenol concentrations (P<0.05. In the isolated heart, exercise increased left ventricular isovolumetric systolic pressure (LVISP; Δ +39 mmHg; P<0.05 from baseline conditions. Hearts from the exercised rats presented a greater response to increasing diastolic pressure. Positive inotropic intervention to calcium and isoproterenol resulted in greater LVISP in exercised animals (P<0.05. The results demonstrated that a single resistance exercise session improved myocardial contractility in SHRs.

  8. Adiponectin alleviates contractile dysfunction of genioglossus in rats exposed to chronic intermittent hypoxia

    Institute of Scientific and Technical Information of China (English)

    WANG Wen-jing; LU Gan; DING Ning; HUANG Han-peng; DING Wen-xiao; ZHANG Xi-long

    2013-01-01

    Background Genioglossal dysfuntion takes an important role in pathogenesis of obstructive sleep apnea hypopnea syndrome (OSAHS) in which chronic intermittent hypoxia (CIH) is the major pathological origin.Recent studies have suggested genioglossal injury induced by CIH might be improved by adiponectin.The aim of this study was to investigate the effects of adiponectin on genioglossus contractile properties in rats exposed to CIH.Methods Thirty-nine healthy male Wistar rats were randomly divided into three groups:normal control (NC),CIH and adiponectin supplement (CIH+Ad) with 13 rats in each.Rats in NC were kept breathing normal air,while rats in CIH and CIH+Ad experienced the same CIH environment eight hours per day for 35 successive days.Rats in CIH+Ad were given intravenous adiponectin of 10 μg twice a week for 30 successive days.Rats in the NC and CIH were injected with normal saline as a control.After 35 days' CIH exposure,the levels of serum adiponectin and genioglossus contractile properties were compared.Results Serum adiponectin level was significantly lower in CIH than in NC (1210 ng/ml vs.2236 ng/ml).Serum adiponectin level in CIH+Ad (1844 ng/ml) was significantly higher than CIH but lower than NC.Twitch tension,time to peak tension,half relaxation time and tetanic tension were significantly lower in CIH than NC and improved in CIH+Ad.All mean tetanic fatigue indices decreased more rapidly in the first 20 seconds than during the subsequent 100 seconds.Tetanic fatigue indices in NC and CIH+Ad were significantly higher compared to CIH.Conclusions CIH could lead to hypoadiponectinaemia,impaired genioglossus contractile properties and decreased fatigue resistance in rats.Such changes could be partially offset by supplementation of adiponectin.

  9. Aspiração manual intrauterina no tratamento do abortamento incompleto até 12 semanas gestacionais: uma alternativa à curetagem uterina Manual vacuum aspiration uterine treatment of incomplete abortion to 12 gestational weeks: an alternative to curettage

    Directory of Open Access Journals (Sweden)

    Marcio Pedroso Saciloto

    2011-10-01

    Full Text Available OBJETIVO: Analisar a eficácia e a ocorrência de complicações, além do tempo de permanência hospitalar e as perdas sanguíneas. MÉTODOS: Trinta pacientes foram selecionadas, alternada e consecutivamente, em um dos grupos (15 no Grupo Curetagem e 15 no Grupo de Aspiração manual intrauterina. As variáveis analisadas foram: eficácia do método, ocorrência de complicações, tempo pré-procedimento, tempo de execução do procedimento, tempo pós-procedimento e tempo total de permanência hospitalar, além de hematócrito e hemoglobina, medidas antes e após o procedimento. As pacientes foram avaliadas clinicamente 10 a 14 dias após o procedimento. Para a análise estatística, foram aplicados testes paramétricos e não-paramétricos e o nível de significância admitido foi de p>0,05. RESULTADOS: Ambos os métodos foram eficazes e não foi registrada nenhuma complicação. As perdas sanguíneas foram semelhantes e o tempo de permanência hospitalar foi significativamente menor no Grupo de Aspiração Manual Intrauterina (p=0,03. CONCLUSÃO: a aspiração manual intrauterina é tão eficaz e segura quanto à curetagem uterina, com a vantagem de necessitar menor tempo de permanência hospitalar, o que aumenta a resolutividade do método, melhorando a qualidade da assistência a essas pacientes.PURPOSE: To analyze the effectiveness and occurrence of complications, in addition to hospitalization time and blood losses. METHODS: Thirty patients were assigned alternatively and consecutively to one of two groups (15 to the Curettage Group and 15 to the Manual Vacuum Aspiration Group. The following variables were analyzed: effectiveness of the method, occurrence of complications, time before the procedure, time of execution of the procedure, time after the procedure, and total time of hospital permanence, in addition to hematocrit and hemoglobin, which were measured before and after the procedure. Patients were evaluated clinically 10 to 14

  10. Importância dos parâmetros seminais nos resultados de inseminação intra-uterina Importance of seminal parameters for intrauterine insemination outcomes

    Directory of Open Access Journals (Sweden)

    Edson Borges Junior

    2003-05-01

    Full Text Available OBJETIVOS: analisar a relevância dos parâmetros seminais nos resultados dos ciclos de inseminação intra-uterina (IIU em pacientes com causa masculina de infertilidade e salientar o caráter prognóstico de cada parâmetro para o sucesso da técnica. MÉTODOS: duzentos e trinta e nove ciclos de IIU (155 casais foram analisados durante período de 15 meses. Todos as pacientes foram submetidas à indução da ovulação de acordo com protocolos do I Consenso Brasileiro de Indução de Ovulação. A análise seminal foi realizada de acordo com os critérios da Organização Mundial da Saúde (OMS para parâmetros como concentração e motilidade e critério estrito de Kruger para avaliação da morfologia. O preparo da amostra para IIU foi realizado por meio de gradientes descontínuos de densidade (ISolate®. Foram formados dois grupos de pacientes de acordo com o resultado de gestação: grupo G - gestação positiva e grupo NG - gestação negativa. RESULTADOS: não houve diferença significativa nos valores obtidos relacionados à concentração total de espermatozóides/mL de sêmen, motilidade total, motilidade de formas progressivas antes e depois do processamento seminal. Quando a morfologia estrita de Kruger foi comparada entre os dois grupos, diferenças significativas foram encontradas (grupo G=10,6% de formas normais; grupo NG=6,4% de formas normais; pPURPOSE: to analyze the influence of seminal parameters on intrauterine insemination (IUI outcomes in patients with male factor and to emphasize the predictive value of each parameter for the successful result. METHODS: two hundred and thirty-nine IUI cycles (155 couples were analyzed for 15 months. Female patients were submitted to ovary hyperstimulation according to the "I Consenso Brasileiro de Indução de Ovulação". Seminal analysis based on the World Health Organization (WHO for sperm concentration and motility was used and sperm morphology was evaluated according to Kruger

  11. Myocardial contractility in the stress echo lab: from pathophysiological toy to clinical tool.

    Science.gov (United States)

    Bombardini, Tonino; Zoppè, Monica; Ciampi, Quirino; Cortigiani, Lauro; Agricola, Eustachio; Salvadori, Stefano; Loni, Tiziana; Pratali, Lorenza; Picano, Eugenio

    2013-01-01

    Up-regulation of Ca2+ entry through Ca2+ channels by high rates of beating is involved in the frequency-dependent regulation of contractility: this process is crucial in adaptation to exercise and stress and is universally known as force-frequency relation (FFR). Disturbances in calcium handling play a central role in the disturbed contractile function in myocardial failure. Measurements of twitch tension in isolated left-ventricular strips from explanted cardiomyopathic hearts compared with non-failing hearts show flat or biphasic FFR, while it is up-sloping in normal hearts. Starting in 2003 we introduced the FFR measurement in the stress echo lab using the end-systolic pressure (ESP)/End-systolic volume index (ESVi) ratio (the Suga index) at increasing heart rates. We studied a total of 2,031 patients reported in peer-reviewed journals: 483 during exercise, 34 with pacing, 850 with dobutamine and 664 during dipyridamole stress echo. We demonstrated the feasibility of FFR in the stress echo lab, the clinical usefulness of FFR for diagnosing latent contractile dysfunction in apparently normal hearts, and residual contractile reserve in dilated idiopathic and ischemic cardiomyopathy. In 400 patients with left ventricular dysfunction (ejection fraction 30 ± 9%) with negative stress echocardiography results, event-free survival was higher (p pressure-volume ratio, ESPVR) ≥ 0.4 mmHg/mL/m2. The prognostic stratification of patients was better with FFR, beyond the standard LV ejection fraction evaluation, also in the particular settings of severe mitral regurgitation or diabetics without stress-induced ischemia. In the particular setting of selection of heart transplant donors, the stress echo FFR was able to correctly select 34 marginal donor hearts efficiently transplanted in emergency recipients. Starting in 2007, we introduced an operator-independent cutaneous sensor to monitor the FFR: the force is quantified as the sensed pre-ejection myocardial

  12. Effect of pinaverium bromide on stress-induced colonic smooth muscle contractility disorder in rats

    Institute of Scientific and Technical Information of China (English)

    Yun Dai; Jian-Xiang Liu; Jun-Xia Li; Yun-Feng Xu

    2003-01-01

    AIM: To investigate the effect of pinaverium bromide, a Ltype calcium channel blocker with selectivity for the gastrointestinal tract on contractile activity of colonic circular smooth muscle in normal or cold-restraint stressed rats and its possible mechanism.METHODS: Cold-restraint stress was conducted on rats to increase fecal pellets output. Each isolated colonic circular muscle strip was suspended in a tissue chamber containing warm oxygenated Tyrode-Ringer solution. The contractile response to ACh or KCl was measured isometrically on inkwriting recorder. Incubated muscle in different concentrations of pinaverium and the effects of pinaverium were investigated on ACh or KCl-induced contraction. Colon smooth muscle cells were cultured from rats and [Ca2+]i was measured in cell suspension using the Ca2+ fluorescent dye fura-2/AlMl.RESULTS: During stress, rats fecal pellet output increased 61% (P<0.01). Stimulated with ACh or KCl, the muscle contractility was higher in stress than that in control. Pinaverium inhibited the increment of [Ca2+]i and the muscle contraction in response to ACh or KCl in a dose dependent manner. A significant inhibition of pinaverium to ACh or KCl induced [Ca2+]i increment was observed at 10-6 mol/L. The IC50 values for inhibition of ACh induced contraction for the stress and control group were 1.66×10-6 mol/L and 0.91×10-6mol/L, respectively. The ICs0 values for inhibition of KCl induced contraction for the stress and control group were 8.13×10-7 mol/L and 3.80×10-7 mol/L, respectively.CONCLUSION: Increase in [Ca2+]i of smooth muscle cells is directly related to the generation of contraction force in colon. L-type Ca2+ channels represent the main route of Ca2+ entry.Pinaverium inhibits the calcium influx through L-type channels;decreases the contractile response to many kinds of agonists and regulates the stress-induced colon hypermotility.

  13. Myocardial ischemia-reperfusion induces upregulation of contractile endothelin ETB receptor in rat coronary arteries

    DEFF Research Database (Denmark)

    Skovsted, Gry Freja; Sheykhzade, Majid; Trautner, Simon; Edvinsson, Lars

    2011-01-01

    situated in the vascular smooth muscle cells mediating vasoconstriction. This study aims to examine whether heart ischemia-reperfusion leads to upregulation of contractile ETB receptors in the smooth muscle layer of the coronary arteries and to investigate the signaling pathways involved in the putative...... compared to vehicle. Immunohistochemical staining displayed enhanced phoshorylation of ERK1/2 after 4 h of incubation and demonstrated ETB receptors in the vascular smooth muscle cells after 7h of incubation. Conclusion The results demonstrate that heart ischemia-reperfusion in rats induces an upregulation...

  14. Barium and strontium as calcium substitutes for contractile responses in the rat tail artery.

    Science.gov (United States)

    Ebeigbe, A B; Aloamaka, C P

    1985-01-01

    The ability of Ba2+ and Sr2+ to substitute for Ca2+ in contractile responses of the rat tail artery has been examined. Both Ba2+ and Sr2+ caused comparable contractions in Ca-depleted NA-stimulated, or K+-depolarized strips. Ba2+ and Sr2+ substitute poorly for Ca2+ at noradrenaline-sensitive membrane sites. At high concentrations, the three divalent cations stabilize the membrane in the order: Ca2+ greater than Sr2+ greater than Ba2+. The relaxation rates following high-K+ contractions were similar for all three divalent cations, suggesting a common mechanism for sequestration/extrusion. PMID:2414057

  15. Enhanced expression of contractile endothelin ET(B) receptors in rat coronary artery after organ culture

    DEFF Research Database (Denmark)

    Johnsson, E.; Maddahi, A.; Wackenfors, A.; Edvinsson, L.

    . Pharmacological inhibition of endothelium-derived dilatory mediators did not influence endothelin ET(A) or ET(B) receptor-mediated vasoconstriction in fresh segments. In cultured arteries, inhibition of endothelial vasodilators potentiated the effect of sarafotoxin 6c. In conclusion, endothelin ET(B) receptor...... stimulation in cultured coronary arteries elicits vasoconstriction. This is likely not related to endothelial dysfunction with putative loss of its vasodilator components, but rather explained by the up-regulation of contractile endothelin ET(B) receptors on smooth muscle cells Udgivelsesdato: 2008/3/17...

  16. Enhanced expression of contractile endothelin ET(B) receptors in rat coronary artery after organ culture

    DEFF Research Database (Denmark)

    Johnsson, Evelina; Maddahi, Aida; Wackenfors, Angelica; Edvinsson, Lars

    . Pharmacological inhibition of endothelium-derived dilatory mediators did not influence endothelin ET(A) or ET(B) receptor-mediated vasoconstriction in fresh segments. In cultured arteries, inhibition of endothelial vasodilators potentiated the effect of sarafotoxin 6c. In conclusion, endothelin ET(B) receptor...... stimulation in cultured coronary arteries elicits vasoconstriction. This is likely not related to endothelial dysfunction with putative loss of its vasodilator components, but rather explained by the up-regulation of contractile endothelin ET(B) receptors on smooth muscle cells....

  17. Effect of thyroid hormone on in vivo contractility of the canine diaphragm.

    Science.gov (United States)

    Miyashita, A; Suzuki, S; Suzuki, M; Numata, H; Suzuki, J; Akahori, T; Okubo, T

    1992-06-01

    This study was designed to examine the effects of long-term (4 wk) administration of thyroid hormone on the in vivo contractility of the canine diaphragm. We implanted a pair of piezoelectric crystals chronically in the left crural and costal parts of the diaphragm by a midline laparotomy. Contractility was assessed by changes in the shortening of muscle fibers after twitch stimulation of both the crural and the costal parts of the diaphragm and in the transdiaphragmatic pressure (Pdi) after tetanic stimulation (10 to 100 Hz). As a reference, we also studied the response of the quadriceps femoris. Pretreatment measurements were taken 2 wk after surgery. Then, dogs assigned to the hyperthyroid group were given thyroid powder, 0.6 g/kg/day, orally for 4 wk. The control group was fed a diet without thyroid powder for 4 wk. Serum free-T4 level (RIA) in the hyperthyroid group (n = 9) increased from 0.68 +/- 0.07 to 5.72 +/- 0.95 ng/dl (mean +/- SE) (p less than 0.01). Pdi decreased 30 to 40% at all frequencies (p less than 0.05) except 10 Hz. Twitch shortening of the crural and costal parts, compared with pretreatment state, decreased significantly by 47.7 +/- 13.1 and 48.1 +/- 15.0%, respectively (p less than 0.05). The maximal rate of relaxation became significantly faster, by 63.5% (p less than 0.05), in the crural part, whereas that of the costal part tended to become faster but not to a significant extent. In the quadriceps femoris, although twitch force showed no change, both the maximal rate of contraction and maximal rate of relaxation became faster, and tetanic force decreased. Histologic examination of hyperthyroid dogs showed vacuolization and loss of fiber area of the diaphragm. These observations suggest that thyroid hormone impairs contractility of both the crural and costal parts of the diaphragm similarly, and that the decrease in contractility may be due to a loss of muscle mass and summation impairment of twitch contraction, which differs from that in

  18. Changes of mitochondria in the contractile cardiomyocytes during postnatal rat ontogenesis

    OpenAIRE

    Kozlov S.V.; Mayevsky A.E.; Mіshalov V.D.; Sulayeva O.N.

    2014-01-01

    Background. CVDs are the number 1 cause of death globally: more people die annually from CVDs than from any other cause. An estimated 17.5 million people died from CVDs in 2012, representing 31% of all global deaths. Of these deaths, an estimated 7.4 million were due to coronary heart disease and 6.7 million were due to stroke. Over three quarters of CVD deaths take place in low- and middle-income countries. Objective. Ultrastructural analysis of mitochondria in the rat contractile cardiomyoc...

  19. Effect of aerobic exercise on the contractile function of gastrocnemius myosin heavy chain

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Objective To study the effect of 4-6 weeks' treadmill training of male SD rats on the contractile function of their gastrocnemius myosin heavy chain (MHC). Methods Forty male SD rats were randomly divided into control group and training group. The treadmill training of the training group rats was incessantly performed for 4-6 weeks at an intensity of about 75% VO2max (18.5-24 m/min,gradient of 0°,each training session lasting 50 minutes,twice a day). The content of gastrocnemius MHC mRNA was tested by rever...

  20. Myosin Isoforms and Contractile Properties of Single Fibers of Human Latissimus Dorsi Muscle

    OpenAIRE

    Antonio Paoli; Pacelli, Quirico F.; Pasqua Cancellara; Luana Toniolo; Tatiana Moro; Marta Canato; Danilo Miotti; Carlo Reggiani

    2013-01-01

    The aim of our study was to investigate fiber type distribution and contractile characteristics of Latissimus Dorsi muscle (LDM). Samples were collected from 18 young healthy subjects (9 males and 9 females) through percutaneous fine needle muscle biopsy. The results showed a predominance of fast myosin heavy chain isoforms (MyHC) with 42% of MyHC 2A and 25% of MyHC 2X, while MyHC 1 represented only 33%. The unbalance toward fast isoforms was even greater in males (71%) than in females (64%...

  1. Effect of cholecystokinin and secretin on contractile activity of isolated gastric muscle strips in guinea pigs

    Institute of Scientific and Technical Information of China (English)

    Wei Li; Tian Zhen Zheng; Song Yi Qu

    2000-01-01

    AIM To study the effect of cholecystokininoctapeptide (CCK-8) and secretin on contractile activity of isolated gastric muscle strips in guinea pigs.METHODS Each isolated gastric muscle strip was suspended in a tissue chamber containing5 mL Krebs solution constantly warmed by water jacked at 37℃ and supplied with a mixed gas of 95% O2 and 5% CO2. After incubating for 1 h under 1 g tension, varied concentrations of CCK-8 and secretin were added respectively in the tissue chamber and the contractile response was measured isometrically on ink-writing recorders.circular and longitudinal muscular tension at rest (fundus LM 19.7%±2.1%, P<0.01; fundus CM 16.7%±2.2%, P<0.01; gastric body LM 16.8% ± 2.3%, P<0.01; body CM 12.7% ± 2.6%,P<0.01; antrum LM 12.3%±1.3%, P<0.01;antrum CM 16.7%±4.5%, P<0.01; pylous CM frequencies of body LM, both LM and CM of antrum and pylorus CM (5.1/min ± 0.2/min to 5.6/min ± 0.2/min, 5.9/min ± 0.2/min to 6.6/min ±0.1/min, 5.4/min ± 0.3/min to 6.3/min ± 0.4/min, 1.3/min ± 0.2/min to 2.3/min ± 0.3/min,amplitude of antral circular muscle (58.6%±pylorus CM (145.0% ± 23.8%, P<0.01), but decrease the mean contractile amplitude of gastric body and antral LM ( - 10.3% ± 3.3%, -10.5% ±4.6%, respectively, P<0.05). All the CCK-8 effects were not blocked by atropine or indomethacin. Secretin had no effect on gastric smooth muscle activity.CONCLUSION CCK-8 possessed both excitatory and inhibitory action on contractile activity of different regions of stomach in guinea pigs. Its action was not mediated via cholinergic M receptor and endogenous prostaglandin receptor.

  2. GABAB receptor-mediated contractile effects resistant to tetrodotoxin in isolated cat ileum.

    Science.gov (United States)

    Pencheva, N; Venkova, K; Radomirov, R

    1990-06-21

    The effects of gamma-aminobutyric acid (GABA) and GABAergic drugs were studied on longitudinal strips from cat terminal ileum prepared after removing the myenteric plexus. GABA and baclofen exerted concentration-dependent contractile effects. Muscimol was ineffective, and bicuculline did not antagonize the effect of GABA. The complete elimination of the neural input to the smooth muscle cells by tetrodotoxin failed to prevent the action of GABA and baclofen. Pharmacological analyses of the effects indicated the existence of GABAB receptors on the smooth muscle cells in the longitudinal layer of cat terminal ileum. PMID:2169425

  3. Contractile responses to ergotamine and dihydroergotamine in the perfused middle cerebral artery of rat

    OpenAIRE

    Tfelt-Hansen, Peer; Nilsson, Elisabeth; Edvinsson, Lars

    2007-01-01

    The vasomotor effects of ergotamine and dihydroergotamine (DHE) on the middle cerebral artery (MCA) of rats were studied using the pressurised arteriography method and in vitro myographs. MCAs from Sprague–Dawley rats were mounted on two glass micropipettes using the arteriograph, pressurised to 85 mmHg and luminally perfused. All vessels used attained spontaneous contractile tone (34.9±1.8% of resting tone) and responded to luminal adenosine triphosphate (ATP) with dilatation (24.1±4.0%), wh...

  4. Gestión intergubernamental y capacidad estratégica en el desarrollo local y regional fronterizo

    OpenAIRE

    José María Ramos García

    2002-01-01

    El artículo tiene como objetivo principal examinar el papel de los gobiernos locales en el desarrollo re g i onal de la frontera norte de México, con base en los enfoques de la gestión interg u b e rnamental de políticas p ú blicas y la capacidad estrat é g i c a . La ap o rtación teórica de este artículo consiste en destacar y especificar el papel de la gestión en las políticas de desarrollo fronterizo, con base en la capacidad de los recursos humanos, administrativa ...

  5. El desafío de la justicia global desde el enfoque de la capacidad de Amartya K. Sen

    OpenAIRE

    Tamarit López, Isabel

    2016-01-01

    La tesis se centra en el estudio del enfoque de la capacidad en el economista Amartya K. Sen y su vinculación con la justicia en el ámbito global. En primer lugar nos centramos en el concepto de justicia global frente al de justicia internacional y transnacional. A continuación nos proponemos analizar el potencial de la capacidad (capability) como clave para una justicia de alcance global. A partir de la crítica de otros autores como Pogge, Nussbaum, Ricoeur, Cortina, Conill y Crocker valoram...

  6. Estudio de viabilidad para el fortalecimiento de las capacidades productivas de la misión de Machanga (Sofala, Mozambique).

    OpenAIRE

    Rodríguez Flamenco, José Alfredo

    2012-01-01

    El “Estudio de Viabilidad para el Fortalecimiento de las Capacidades Productivas de la misión de Machanga (Sofala, Mozambique)” propone, define y evalúa, desde el punto de vista técnico y económico, las acciones necesarias que se deberían implementar para fortalecer las capacidades productivas del sector agrícola de la misión de Machanga, gestionada por la Asociación ESMABAMA. Para alimentar la población estudiantil que tiene en régimen interno, ESMABAMA necesita unas 400 to...

  7. Capacidad aeróbica, felicidad y satisfacción con la vida en adolescentes españoles

    OpenAIRE

    Juan A. Jim\\u00E9nez-Moral; Mar\\u00EDa L. Zagalaz S\\u00E1nchez; David Molero; Manuel Pulido-Martos; Ruiz, Jonatan R.

    2013-01-01

    Objetivos: Analizar la asociación entre capacidad aeróbica, felicidad subjetiva y satisfacción con la vida en adolescentes. Método: Participaron 388 adolescentes (207 mujeres) de 12-18 años de edad. La capacidad aeróbica se evaluó mediante el test de 20 metros de ida y vuelta. La felicidad subjetiva y la satisfacción con la vida se evaluaron mediante las escalas Subjective Happiness Scale y Satisfaction With Life Scale, respectivamente. Se midió el peso y la talla de los adolescentes y se cal...

  8. CAPACIDAD DE HIDRATACIÓN DE LOS CEREALES PARA DESAYUNO KELLOGG'S CAPACITY OF HYDRATATION OF KELOGG'S EXPANDED CEREALS

    OpenAIRE

    Francisco Prieto G; Judith Prieto M; Alma Delia Román; Alberto José Gordillo M; Carlos Gómez A

    2005-01-01

    Los cereales expuestos a la libre adsorción de humedad, alcanzan rápidamente el equilibrio de adsorción. A baja aW (0.10), los tres cereales para desayuno evaluados en este trabajo, ceden humedad al medio; a partir de valores de aW de 0.53 y mayores manifiestan sus capacidades de adsorción de humedad con el tiempo, lo cual está asociado a que en los cereales se encontraron altos contenidos de azúcares. El cereal de arroz, manifestó los menores valores de capacidad de adsorción, en tanto el de...

  9. Capacidad de uso del suelo y usos recomendados en la Sierra de Espadán (Castellón)

    OpenAIRE

    Martínez, Victoria; Carbó, Ester

    1992-01-01

    Se determinan la capacidad de uso de los suelos de la Sierra de Espadán y los usos recomendados como metodologías para evaluación de dicho territorio. Se exponen, con el código correspondiente, las limitaciones del suelo y del entorno que condicionan cada uno de los usos propuestos. Se presentan el mapa de Capacidad de uso y la Cartografía de las Recomendaciones de uso en un sector representativo de la Sierra de Espadán. Se obtiene que el 90% del total de la superficie prese...

  10. ¿Einstein o Dalí?: altas capacidades e inteligencias múltiples, visión actual.

    OpenAIRE

    Herrera-Parejo, Clara Isabel

    2015-01-01

    La presente investigación trata de acercarse al conocimiento y características propias de los alumnos con altas capacidades intelectuales y su relación con el modelo de las inteligencias múltiples de Howard Gardner, como propuesta que favorece la educación personalizada y el desarrollo de las capacidades y talentos en los niños más dotados, atendiendo a sus necesidades; además de cómo se recoge la atención a estos alumnos en nuestra legislación, algunos programas de intervención en el aula, e...

  11. Diseño de un programa de intervención con alumnos de altas capacidades: Optimismo

    OpenAIRE

    Martínez San José, Bárbara

    2015-01-01

    Este documento presenta un proyecto de intervención para favorecer el optimismo en alumnos con altas capacidades en edades comprendidas entre 12 y 16 años. En un primer momento se han expuesto los objetivos a conseguir con este trabajo. Seguidamente, se han definido con los conceptos de altas capacidades y de optimismo y sentido positivo. Así mismo, se ha explicado en qué contexto y que objetivos tendrá el programa de intervención de optimismo en sí, y se ha desarrollado con diversas activida...

  12. Alumnos con altas capacidades: protocolo de actuación y propuesta de actividades de enriquecimiento curricular

    OpenAIRE

    Aguirre Estarli, Laura

    2016-01-01

    Los alumnos con altas capacidades intelectuales son considerados legalmente como alumnos con necesidades específicas de apoyo educativo, y requieren, por lo tanto, una atención distinta a la ordinaria para que logren el pleno desarrollo de sus capacidades. Sin embargo, en la práctica educativa son los grandes olvidados; apenas se les diagnostica como tales, no se les suele prestar la ayuda que precisan, de modo que un alto porcentaje de niños superdotados presentan fracaso escolar. Aparte de ...

  13. Perfil y necesidades de alumnos universitarios con altas capacidades en la Universidad de Málaga. Datos iniciales

    OpenAIRE

    Fernández Molina, Milagros

    2016-01-01

    La investigación sobre la presencia en las Universidad de alumnos con altas capacidades intelectuales es muy escasa, constituyendo un tema de estudio de importante proyección. Apenas hay publicaciones o informes sobre el acceso a los estudios superiores del alumnado con alta capacidad, el perfil personal e intelectual que presenta, las necesidades que tiene durante su trayectoria universitaria, la tasa de abandonos o de éxito en los estudios universitarios que elige, etc. En este trabajo se p...

  14. Capacidades para el desarrollo rural de grupos de mujeres de cuatro comunidades en la Sierra Nevada de Puebla.

    OpenAIRE

    Ramírez Castel, Verónica

    2014-01-01

    Esta investigación tuvo como objetivo el estudio de los tipos de capacidades de las mujeres rurales participantes en la Asociación para el Desarrollo “Enlace Sierra Nevada, A. C.”, sus efectos en los ámbitos personal, familiar y comunitario y los aspectos que favorecen y limitan este desarrollo de capacidades. El método general de investigación fue el descriptivo explicativo en el que se usaron métodos particulares como las entrevistas a profundidad a las mujeres participantes en los proyecto...

  15. CAPACIDAD PREDICTIVA DE MODELOS DE INSOLVENCIA CON BASE EN NÚMEROS CONTABLES Y DATOS DESCRIPTIVOS

    Directory of Open Access Journals (Sweden)

    Júlio Orestes da Silva

    2012-09-01

    Full Text Available En el Brasil la pesquisa sobre modelos de previsión de insolvencia se inició en la década de 1970, siendo que la mayor parte de los trabajos hizo uso del Análisis Discriminante como herramienta estadística del modelo. En los últimos años, se buscó verificar si es posible prever la insolvencia de las empresas utilizando datos descriptivos contenidos en los informes de las organizaciones. Así, este estudio tiene como objetivo verificar la capacidad de algunos modelos de previsión de insolvencia en prever la discontinuidad de empresas brasileñas que decretaron quiebra. La pesquisa se caracteriza como descriptiva y posee abordaje cuantitativo, realizado por medio de pesquisa documental. La muestra totalizó 13 empresas que decretaron quiebra entre los años de 1997 y 2003. Los resultados indican que la mayoría de los modelos de previsión de quiebra testados presentó resultados elevados de previsiones correctas. Los modelos de previsión de discontinuidad con base en informes descriptivos obtuvieron en media más previsiones asertivas en relación a la capacidad de prever la quiebra de las empresas. Esos descubrimientos demuestran que, a pesar de que algunas pesquisas apunten hacia la falta de validez de predictores creados en realidades empresariales diferentes, algunos modelos todavía poseen buena capacidad de previsión de insolvencia. Se concluye que tanto los modelos de previsión de insolvencia con base en números contables en relación a los modelos que se utilizan datos de informes descriptivos pueden prever la discontinuidad de las organizaciones. Por fin, se puede inferir que la mayoría de los modelos de previsión de quiebra que hacen uso de números contables pueden ser funcionales y capaces de prever la discontinuidad de las organizaciones.

  16. Treinamento aerobio e resistido, qualidade de vida e capacidade funcional de hipertensas

    Directory of Open Access Journals (Sweden)

    Thais Inacio Rolim Povoa

    2014-01-01

    Full Text Available INTRODUÇÃO: Pacientes hipertensos podem apresentar comprometimento da qualidade de vida (QV e da qualidade de vida relacionada à saúde (QVRS, tanto pela hipertensão arterial (HA, quanto pelos eventuais efeitos adversos do tratamento. Exercícios físicos, aeróbios e resistidos, melhoram a performance cardiorrespiratória e neuromuscular, mas há poucas evidências sobre seus efeitos na QV, QVRS e capacidade funcional (CF em mulheres hipertensas. OBJETIVO: Avaliar e comparar os efeitos dos treinamentos aeróbio e resistido sobre a QV, QVRS e a capacidade funcional em hipertensas. MÉTODOS: Ensaio clínico randomizado, cego, com total de 18 sessões de exercícios. Foram incluídas mulheres hipertensas sob tratamento medicamentoso, não participantes de programas de exercícios, com 50 anos de idade ou mais, que não apresentaram arritmias e/ou alterações isquêmicas em teste ergométrico (protocolo de Bruce. A amostra foi randomizada como segue: grupo aeróbio (GA (n = 21 e grupo resistido (GR (n = 20. Intensidade GA: leve a moderada (Escala de Borg adaptada; GR: até 50-65% de 1 RM. Duas séries com 12 a 15 repetições. Antes e após a intervenção foram aplicados os questionários WHOQOL-bref (QV e SF-36 (QVRS e realizado o teste de caminhada de 6 minutos (TC6` para avaliar a CF. Estatística: testes Shapiro-Wilk, t de Student, Fisher, U de Mann-Whitney e Wilcoxon. Nível de significância: p 0,05. Após a intervenção houve melhora significativa em todos os domínios do WHOQOL-bref no GA e no GR houve melhora no domínio aspectos físicos. No SF-36, constatou-se melhora significativa em sete de oito domínios tanto no GA quanto no GR. Capacidade funcional: verificou-se melhora nos dois grupos (p < 0,001. CONCLUSÃO: Os dois tipos de treinamento melhoraram a QV e a CF e, dependendo dos objetivos estabelecidos, ambos podem ser eficazes.

  17. Gravid med spontan ruptur af arteria uterina

    DEFF Research Database (Denmark)

    Jònsdòttir, Fjòla; Pinborg, Anja; Wilken-Jensen, Charlotte

    2014-01-01

    Pregnant women with acute abdominal pain are a clinical challenge. We present a rare but potential life-threatening condition of a pregnant woman with acute abdominal pain. The woman was in gestational week 37 with severe abdominal pain and was admitted to the labour ward. She became haemo......-dynamic instable 24 hours after vaginal delivery, and emergency laparotomi revealed a spontaneous rupture of the right uterine artery. Spontaneous rupture of the uterine artery is rare but should be considered as a possible cause of acute abdominal pain in pregnant women....

  18. Consideraciones para el desarrollo de capacidades de investigación en universidades privadas

    Directory of Open Access Journals (Sweden)

    Maria Luisa Isla

    2013-01-01

    Full Text Available Se presentan reflexiones y estrategias que contribuyan a formar capacidades de investigación en universidades privadas con investigadores jóvenes comprometidos con el avance del conocimiento en su área de investigación así como con su entorno natural y soc ial. Mediante una combinación de formación en el aula y formación experiencial, se presentan las habilidades investigativas a desarrollar y los ejes de formación universitaria que contribuyen a formar investigadores. Adicionalmente a esta formación básica se plantea la necesidad de generar las condiciones para desarrollar las habilidades de investigación en la práctica, laborando en áreas experimentales en las que se colabore con grupos de investigación activos.

  19. Capacidad predictiva del apoyo social en la inteligencia emocional de adolescentes

    Directory of Open Access Journals (Sweden)

    Lorea Azpiazu

    2015-06-01

    Full Text Available El objetivo de este estudio es analizar la relación entre el apoyo social (familiar, amistades y profesorado y la inteligencia emocional (atención, claridad y reparación. La muestra está compuesta por 1543 adolescentes, 728 chicos (47.18% y 815 chicas (52.81%, entre los 11 y 18 años (Medad = 14.24, DT = 1.63. Los análisis estadísticos se llevan a cabo con el programa SPSS 21 para Windows. Los resultados del análisis correlacional señalan que el apoyo social y la inteligencia emocional están significativamente relacionados en ambos sexos. Por otro lado, el análisis de regresión demuestra la capacidad predictiva de los diferentes apoyos en la inteligencia emocional tanto en la muestra masculina como en la femenina.

  20. Enciclomedia como Recurso Didáctico en Niños con Capacidades Diferentes

    Directory of Open Access Journals (Sweden)

    Sara Puentes Jiménez

    2007-01-01

    Full Text Available El presente artículo describe una experiencia que ilustra el impacto de la tecnología educativa moderna sobre la educación especial. La experiencia se realizó en el CAM VII (Centro de Atención Múltiple de Aguascalientes, que ofrece servicios educativos a niños con NEE (Necesidades Educativas Especiales. Se eligió un grupo de niños disfuncionales y se seleccionó un tema de historia de México, el cual fue explicado usando como apoyo didáctico el programa Enciclomedia. Los resultados fueron positivos si bien difíciles de cuantificar. Es importante resaltar que los niños con NEE tienen derecho y capacidad para usar tecnología educativa moderna en beneficio de su educación integral.

  1. Fortaleciendo las capacidades presupuestarias de los Parlamentos en América Latina y el Caribe

    OpenAIRE

    Carlos Santiso; Marco Varea

    2013-01-01

    En la última década, los Parlamentos han mostrado cada vez más interés y han tenido más protagonismo en el proceso presupuestario al demandar mayor información sobre el desempeño del gobierno en su gestión de los recursos públicos. Sin embargo, en América Latina y el Caribe los Parlamentos evidencian desafíos para intervenir en el proceso presupuestario y las políticas fiscales. Su capacidad de incidencia en materia presupuestaria -y más generalmente en la política fiscal y la gestión financi...

  2. Comando e Controlo, um potenciador das capacidades da Artilharia Antiaérea

    OpenAIRE

    Loureiro, Ricardo

    2012-01-01

    O presente Trabalho de Investigação Aplicada tem como finalidade investigar e analisar as capacidades e requisitos que uma Bateria de Artilharia Antiaérea tem, ao nível do Comando e Controlo, para participar em missões no âmbito da Organização do Tratado do Atlântico Norte e em missões no território nacional. A parte prática do trabalho teve lugar no Regimento de Artilharia Antiaérea nº 1, tendo por base, análise de documentos, entrevistas e relatos de experiências pessoais d...

  3. Mulheres mastectomizadas : acesso à informação e aprendizagem de capacidades

    OpenAIRE

    Costa, Ilídia

    2011-01-01

    O cancro da mama e tratamento adjuvantes acarretam para a mulher uma série de consequências de índole física e psicológica vastamente descritos na literatura. Alterações ao nível da concepção da saúde e das políticas de saúde quer a nível nacional quer internacional, centram o processo de cuidados na pessoa como parceira activa, detentora de capacidades e como tal responsável pela tomada de decisão livre e esclarecida sobre a sua saúde e tratamento. Na mulher mastectomizada com linfadenectomi...

  4. Capacidade funcional e bomba muscular venosa na doença venosa crónica

    OpenAIRE

    Gonçalves, Catarina; Crisóstomo, Rute

    2015-01-01

    Objetivos – Avaliar a capacidade funcional e a função da Bomba Muscular Venosa em sujeitos com Doença Venosa Crónica (DVC) e em saudáveis. Relevância – Os utentes com DVC têm diminuição da qualidade de vida relacionada com a saúde e alterações funcionais dos membros inferiores sendo importante avaliar e intervir nesta área. Amostra – Participaram neste estudo 109 sujeitos (36 saudáveis e 73 com DVC). Material e Métodos – A severidade da DVC foi avaliada através da Venous Clinical Sev...

  5. La capacidad de actualizar información inferencial en la lectura

    Directory of Open Access Journals (Sweden)

    Ana I. Pérez Muñoz

    2013-10-01

    Full Text Available Durante la comprensión lectora, el proceso de actualización inferencial nos permite sustituir una idea del texto que hayamos generado equívocamente por otra más plausible que aún está por confirmar. Esta habilidad parece depender de nuestra capacidad de Memoria de Trabajo, la cual nos ayuda a mantener una representación coherente de lo que estamos leyendo, mientras que comprendemos la información posterior y descartamos ideas que hemos podido interpretar de forma errónea. El entrenamiento en actualización inferencial a edades tempranas podría beneficiar la comprensión lectora de textos a lo largo de toda la vida.

  6. Capacidad de transporte de sedimentos en cuencas con información escasa.

    OpenAIRE

    Posada García, Lilian; Montoya Jaramillo, Luis Javier

    2000-01-01

    Existe en la literatura un gran número de metodologías para estimar la capacidad de una corriente para transportar sedimentos y se necesitan suficientes registros simultáneos de caudal líquido y sólido para poder seleccionar el método de estimación más apropiado para una corriente determinada. Después de aplicar distintas metodologías a corrientes del departamento de Antioquia, en la zona Andina Colombiana, se desarrolla un nuevo método para el cálculo de la carga en suspensión y de la car...

  7. PLANIFICACIÓN JERÁRQUICA CON ANÁLISIS DE CARGA-CAPACIDAD

    Directory of Open Access Journals (Sweden)

    Tulio Gerardo Motoa

    2001-01-01

    Full Text Available

    La planificación jerárquica de múltiples proyectos, surge como una opción robusta y potente, para resolver el problema de gestión del sistema global. La propuesta se basa en la construcción de una estructura de descomposición-agregación de los proyectos y los recursos, con múltiples niveles, en la cual, los niveles más altos presentan los datos más agregados, con horizontes de tiempo más largos y los niveles inferiores son más detallados y con horizontes más cortos. Dentro del ámbito de la planificación jerárquica, el análisis de carga-capacidad pretende fundamentalmente mejorar la relación entre la oferta y la demanda de recursos en los procesos de planificación de proyectos, de tal manera que se garantice el cumplimiento de las fechas ya establecidas. Este análisis, se implementa midiendo algunos indicadores que reflejan el estado de la relación entre la carga y la capacidad y ejecutando un procedimiento de nivelación que avanza en el equilibrio de tal estado. El procedimiento de análisis se ha experimentado mediante un conjunto de proyectos generados aleatoriamente y los resultados son bastante buenos.

  8. Comportamientos y capacidad de agencia de autocuidado de adultos con insuficiencia cardiaca

    Directory of Open Access Journals (Sweden)

    EDITH ARREDONDO HOLGUÍN

    2010-06-01

    Full Text Available Este estudio tiene como objetivo describir comportamientos y capacidad de agencia de autocuidado de pacientes con insuficiencia cardiaca de una clínica cardiovascular de Medellín entre noviembre de 2007 y mayo de 2008. Es un estudio descriptivo con abordaje cuantitativo. La muestra fue de 206 adultos con insuficiencia cardiaca diagnosticados por sintomatología clínica, fracción de eyección menor o igual a 40%. Se usaron para la recolección de datos la Escala Capacidad de Agencia de Autocuidado desarrollada por Isemberg y Evers et ál. (1993, traducida por Gallego, que mide el nivel de agencia de autocuidado, y la Escala Comportamientos de Autocuidado validada en Colombia en 2007 por Arredondo, que mide la frecuencia de comportamientos de autocuidado de adultos con insuficiencia cardiaca en la solicitud de ayuda, adaptación a vivir con la enfermedad y el régimen terapéutico, modificación del autoconcepto y aceptación de sí mismo, vivir con efectos de la insuficiencia cardiaca y el tratamiento. Concluye que el mayor número de pacientes eran hombres; que factores básicos condicionantes como el apoyo de la familia, desempeño de un trabajo, afiliación al sistema de salud, nivel socioeconómico y acceso a un nivel educativo fueron importantes para los pacientes. La frecuencia de comportamientos de autocuidado fue media en los adultos en la mayoría de las dimensiones y el nivel de agencia fue alto y muy alto. Igualmente se concluyó que no hay una correlación entre agencia y comportamientos de autocuidado. Lo que plantea que los adultos tienen el conocimiento pero no han tomado la decisión de realizar acciones de autocuidado.

  9. Capacidad de reserva de agua en los suelos del Borde Norte de Bogotá

    Directory of Open Access Journals (Sweden)

    Yolima del C. Agualimpia Dualiby

    2013-01-01

    Full Text Available La información aquí presentada corresponde a resultados parciales de la investigación que se adelanta sobre procesos hidrodinámicos que afectan los suelos de la sabana de Bogotá -Colombia y cuyo propósito principal es el cálculo e interpretación de la capacidad de reserva hídrica en los suelos de la reserva forestal borde norte de Bogotá, con el fin de investigar sobre la susceptibilidad a la desertificación que presentan las aproximadamente 1.600 hectáreas que la componen. El análisis se realizó con 407 datos recogidos en campo, geo-referenciados y 17 perfiles de suelos descritos en el área. Como producto de este análisis, se definieron diecisiete áreas hidrológicas de acuerdo a la sectorización morfo-pictórica, con base en la descripción de varios perfiles de suelos dentro de la misma unidad geomorfológica. Se encontró como limitante de la capacidad de almacenamiento del suelo, la compactación natural de algunas de sus capas, cuyos resultados fueron comparados con la humedad natural para verificar el grado de densificación que las afecta. Se efectuó también la descripción de la geometría del poro del suelo y su incidencia en la retención de humedad.

  10. Capacidade combinatória em mamoeiro para resistência a oídio

    Directory of Open Access Journals (Sweden)

    Marcelo Vivas

    2012-01-01

    Full Text Available A resistência genética constitui alternativa sustentável para o controle do oídio. Objetivando indicar possíveis combinações híbridas que contribuam para a redução da severidade de oídio em folha de mamoeiro, foi realizado um cruzamento dialélico envolvendo oito genótipos, sendo quatro do grupo 'Solo' e quatro do grupo 'Formosa'. Os 56 híbridos (F1's e recíprocos juntamente com seus genitores foram avaliados em blocos casualizados com quatro repetições. A severidade do oídio na folha foi quantificada em março e maio de 2010, respectivamente, aos 11 e 13 meses após o plantio. Com a média de cada tratamento foram estimadas as capacidades geral e específica de combinação. Assim, considerando as estimativas da capacidade combinatória obtidas com base na média das duas avaliações, as melhores combinações para os genitores avaliados quanto à severidade do oídio foram 'Maradol x Waimanalo', 'Maradol x Sunrise Solo 72/12', 'JS 12-4 x São Mateus', 'Sekati x Waimanalo', 'Sekati x Golden', 'Sekati x Sunrise Solo 72/12', 'Sekati x São Mateus', 'Waimanalo x São Mateus' e 'Golden x São Mateus'. Os resultados evidenciam ainda a possibilidade de obtenção de híbridos com potencial para redução da severidade do oídio provenientes de cruzamentos entre genótipos dos grupos 'Solo' e 'Formosa' e também em cruzamentos dentro do grupo 'Solo'.

  11. ¿Es posible potenciar la capacidad de liderazgo en la universidad?

    Directory of Open Access Journals (Sweden)

    C. Delia Dávila Quintana

    2015-04-01

    Full Text Available El objetivo del trabajo es analizar la importancia de la educación superior, concretamente de los métodos de enseñanza-aprendizaje, en el comportamiento posterior de los graduados en el puesto de trabajo en relación a tres dimensiones del liderazgo: liderazgo orientado a las tareas, a las relaciones y al cambio. Las competencias profesionales desarrolladas por los graduados durante sus estudios son el canal de transmisión desde la educación al futuro desempeño como líderes. Para ello se estimaron modelos de ecuaciones estructurales (SEM con una muestra de aproximadamente 3.500 jóvenes graduados por universidades españolas provenientes de la base de datos reflex. Los resultados obtenidos indican que el comportamiento como líderes en las tres dimensiones analizadas depende crucialmente del nivel competencial de los individuos en determinadas competencias clave. Las competencias relacionadas con la capacidad de liderazgo son desarrolladas durante los estudios universitarios y posteriormente ampliadas a través de la experiencia y formación en el puesto. El análisis también identifica los métodos de enseñanza-aprendizaje que presentan una mayor influencia en el desarrollo de las competencias para el liderazgo. La principal implicación del análisis consiste en que un entorno educativo adecuado puede favorecer la adquisición durante los estudios de las competencias necesarias para el ejercicio de la capacidad de liderazgo, lo que a su vez favorece el desarrollo posterior de estas mismas competencias en las primeras experiencias laborales, incrementando así la propensión de los graduados a actuar efectivamente como líderes en las organizaciones para las que trabajan en las tres dimensiones del liderazgo analizadas.

  12. Commonly used intravenous anesthetics decrease bladder contractility: An in vitro study of the effects of propofol, ketamine, and midazolam on the rat bladder

    Science.gov (United States)

    Ceran, Canan; Pampal, Arzu; Goktas, Ozgur; Pampal, H. Kutluk; Olmez, Ercument

    2010-01-01

    Aim: This study was designed to test the hypothesis that propofol, ketamine, and midazolam could alter the contractile activity of detrusor smooth muscle. Materials and Methods: Four detrusor muscle strips isolated from each rat bladder (n = 12) were placed in 4 tissue baths containing Krebs-Henseleit solution. The carbachol (10 −8to 10−4mol/L)-induced contractile responses as well as 5, 10, 20, 30, 40, 50 Hz electrical field stimulation (EFS)-evoked contractile responses of the detrusor muscles were recorded using isometric contraction measurements. After obtaining basal responses, the in vitro effects of propofol, ketamine, midazolam (10−5 to 10−3 mol/L), and saline on the contractile responses of the detrusor muscle strips were recorded and evaluated. Results: All the 3 drugs reduced the carbachol-induced and/or EFS-evoked contractile responses of rat detrusor smooth muscles in different degrees. Midazolam (10−4 to 10−3 mol/L) caused a significant decrease in the contractile responses elicited by either EFS or carbachol (P=0.000−0.013). Propofol (10−3mol/L) caused a decrease only in EFS-evoked contractile responses (P=0.001−0.004) and ketamine (10−3mol/L) caused a decrease only in carbachol-induced contractile responses (P=0.001−0.034). Conclusion: We evaluated the effects of the 3 different intravenous anesthetics on detrusor contractile responses in vitro and found that there are possible interactions between anesthetic agents and detrusor contractile activity. The depressant effects of midazolam on the contractile activity were found to be more significant than ketamine and propofol. Despite the necessity of further studies, it could be a piece of wise advice to clinicians to keep the probable alterations due to intravenous anesthetics in mind, while evaluating the results of urodynamic studies in children under sedation. PMID:21116355

  13. Selective increase of the contractile response to endothelin-1 in subcutaneous arteries from patients with essential hypertension

    DEFF Research Database (Denmark)

    Lind, H; Adner, M; Erlinge, D;

    1999-01-01

    arteries from subjects with established essential hypertension with matched controls. Furthermore, with RT-PCR, the occurrence of mRNA for the ETA and ET(B) receptors was shown in the tunica media layer of subcutaneous arteries in controls and hypertensives. The maximum contractile response to endothelin-1...... was significantly higher in the subcutaneous arteries of the hypertensives (by 88% with no change in potency) as compared to controls. The responses to noradrenaline, acetylcholine and potassium chloride did not differ between the groups. This selective increase in the contractile response to......Endothelin-1 has been shown to contribute to basal vascular tone in man. Since endothelin-1 is a potent vasoconstrictor putatively involved in hypertension, we have compared the contractile responses of endothelin-1 and noradrenaline in relation to potassium chloride in subcutaneous resistance...

  14. Abnormal glucose metabolism is associated with reduced left ventricular contractile reserve and exercise intolerance in patients with chronic heart failure

    DEFF Research Database (Denmark)

    Egstrup, M; Kistorp, C N; Schou, M;

    2013-01-01

    AIMS: To investigate the associations between glucose metabolism, left ventricular (LV) contractile reserve, and exercise capacity in patients with chronic systolic heart failure (HF). METHODS AND RESULTS: From an outpatient HF clinic, 161 patients with systolic HF were included (mean age 70 ± 10...... years, 69% male, 59% had ischaemic heart disease, mean LV ejection fraction (LVEF) 37 ± 9%). Thirty-four (21%) patients had known diabetes mellitus (DM). Oral glucose tolerance testing (OGTT) classified patients without a prior DM diagnosis as normal glucose tolerance (NGT), impaired glucose tolerance...... (467 m) (P <0.001). Differences in clinical variables, resting echocardiographic parameters or contractile reserve, did not explain the exercise intolerance related to diabetes. CONCLUSION: Diabetes, known or newly detected by OGTT, is independently associated with reduced LV contractile reserve and...

  15. Contractile effects of 3,4-methylenedioxymethamphetamine on the human internal mammary artery.

    Science.gov (United States)

    Silva, Sónia; Carvalho, Félix; Fernandes, Eduarda; Antunes, Manuel J; Cotrim, Maria Dulce

    2016-08-01

    Since the late 1980s numerous reports have detailed adverse reactions to the use of 3,4-methylenedioxymethamphetamine (MDMA) associated with cardiovascular collapse and sudden death, following ventricular tachycardia and hypertension. For a better understanding of the effects of MDMA on the cardiovascular system, it is critical to determine their effects at the vasculature level, including the transporter or neurotransmitter systems that are most affected at the whole range of drug doses. With this purpose in mind, the aim of our study was to evaluate the contractile effect of MDMA in the human internal mammary artery, the contribution of SERT for this effect and the responsiveness of this artery to 5-HT in the presence of MDMA. We have also studied the possible involvement of 5-HT2 receptors on the MDMA contractile effect in this human blood vessel using ketanserin. Our results showed that MDMA contracted the studied human's internal mammary artery in a SERT-independent form, through activation of 5-HT2A receptors. Considering the high plasma concentrations achieved in heavy users or in situations of acute exposure to drugs, this effect is probably involved in the cardiovascular risk profile of this psychostimulant, especially in subjects with pre-existing cardiovascular disease. PMID:27079619

  16. Dynamics of myosin II organization into contractile networks and fibers at the medial cell cortex

    Science.gov (United States)

    Nie, Wei

    The cellular morphology of adhered cells depends crucially on the formation of a contractile meshwork of parallel and cross-linked stress fibers along the contacting surface. The motor activity and mini-filament assembly of non-muscle myosin II is an important component of cell-level cytoskeletal remodeling during mechanosensing. To monitor the dynamics of non-muscle myosin II, we used confocal microscopy to image cultured HeLa cells that stably express myosin regulatory light chain tagged with GFP (MRLC-GFP). MRLC-GFP was monitored in time-lapse movies at steady state and during the response of cells to varying concentrations of blebbistatin (which disrupts actomyosin stress fibers). Using image correlation spectroscopy analysis, we quantified the kinetics of disassembly and reassembly of actomyosin networks and compared to studies by other groups. This analysis suggested the following processes: myosin minifilament assembly and disassembly; aligning and contraction; myosin filament stabilization upon increasing contractile tension. Numerical simulations that include those processes capture some of the main features observed in the experiments. This study provides a framework to help interpret how different cortical myosin remodeling kinetics may contribute to different cell shape and rigidity depending on substrate stiffness. We discuss methods to monitor myosin reorganization using non-linear imaging methods.

  17. Model of myosin node aggregation into a contractile ring: the effect of local alignment

    Energy Technology Data Exchange (ETDEWEB)

    Ojkic, Nikola; Vavylonis, Dimitrios [Department of Physics, Lehigh University, Bethlehem, PA 18015 (United States); Wu Jianqiu, E-mail: vavylonis@lehigh.edu [Department of Molecular Genetics and Department of Molecular and Cellular Biochemistry, Ohio State University, Columbus, OH 43210 (United States)

    2011-09-21

    Actomyosin bundles frequently form through aggregation of membrane-bound myosin clusters. One such example is the formation of the contractile ring in fission yeast from a broad band of cortical nodes. Nodes are macromolecular complexes containing several dozens of myosin-II molecules and a few formin dimers. The condensation of a broad band of nodes into the contractile ring has been previously described by a search, capture, pull and release (SCPR) model. In SCPR, a random search process mediated by actin filaments nucleated by formins leads to transient actomyosin connections among nodes that pull one another into a ring. The SCPR model reproduces the transport of nodes over long distances and predicts observed clump-formation instabilities in mutants. However, the model does not generate transient linear elements and meshwork structures as observed in some wild-type and mutant cells during ring assembly. As a minimal model of node alignment, we added short-range aligning forces to the SCPR model representing currently unresolved mechanisms that may involve structural components, cross-linking and bundling proteins. We studied the effect of the local node alignment mechanism on ring formation numerically. We varied the new parameters and found viable rings for a realistic range of values. Morphologically, transient structures that form during ring assembly resemble those observed in experiments with wild-type and cdc25-22 cells. Our work supports a hierarchical process of ring self-organization involving components drawn together from distant parts of the cell followed by progressive stabilization.

  18. Dietary nitrate improves cardiac contractility via enhanced cellular Ca(2+) signaling.

    Science.gov (United States)

    Pironti, Gianluigi; Ivarsson, Niklas; Yang, Jiangning; Farinotti, Alex Bersellini; Jonsson, William; Zhang, Shi-Jin; Bas, Duygu; Svensson, Camilla I; Westerblad, Håkan; Weitzberg, Eddie; Lundberg, Jon O; Pernow, John; Lanner, Johanna; Andersson, Daniel C

    2016-05-01

    The inorganic anion nitrate (NO3 (-)), which is naturally enriched in certain vegetables (e.g., spinach and beetroot), has emerged as a dietary component that can regulate diverse bodily functions, including blood pressure, mitochondrial efficiency, and skeletal muscle force. It is not known if dietary nitrate improves cardiac contractility. To test this, mice were supplemented for 1-2 weeks with sodium nitrate in the drinking water at a dose similar to a green diet. The hearts from nitrate-treated mice showed increased left ventricular pressure and peak rate of pressure development as measured with the Langendorff heart technique. Cardiomyocytes from hearts of nitrate-treated and control animals were incubated with the fluorescent indicator Fluo-3 to measure cytoplasmic free [Ca(2+)] and fractional shortening. Cardiomyocytes from nitrate-treated mice displayed increased fractional shortening, which was linked to larger Ca(2+) transients. Moreover, nitrate hearts displayed increased protein expression of the L-type Ca(2+) channel/dihydropyridine receptor and peak L-type Ca(2+) channel currents. The nitrate-treated hearts displayed increased concentration of cAMP but unchanged levels of cGMP compared with controls. These findings provide the first evidence that dietary nitrate can affect the expression of important Ca(2+) handling proteins in the heart, resulting in increased cardiomyocyte Ca(2+) signaling and improved left ventricular contractile function. Our observation shows that dietary nitrate impacts cardiac function and adds understanding to inorganic nitrate as a physiological modulator. PMID:27071401

  19. Scintigraphic measurement of the contractile activity of the gastric antrum using factor analysis

    International Nuclear Information System (INIS)

    The motor activity of the gastric antrum is difficult to record by manometric means and scintigraphic methods have proved unsatisfactory so far as no consistent relationship between antral contractile activity and gastric emptying rate could be detected. We investigated, using data recorded in 16 healthy human subjects after the ingestion of a semisolid standard meal, whether a newly developed method employing factor analysis would yield more meaningful and reproducible results. Factor analysis was applied to sequential scintigraphic images (3-s frame time) of gastric antrum. The computed factor images and the respective factor curves are representative of distinct dynamic structures of the antrum. From the more or less sinusoidal excursions of the factor curves, which exhibited the 3 cycles per minute frequency characteristic for the stomach, amplitude, frequency and propagation velocity of antral contractions can be calculated. The amplitudes of the factor curves were used to calculate a contraction index. This contraction index was found to be correlated significantly negatively with the gastric half-emptying time of the ingested meal. The employed factor analytical approach thus seems a promising tool to further investigate the role of antral contractility in the process of gastric emptying. (Authors)

  20. Contractile properties of rat, rhesus monkey, and human type I muscle fibers

    Science.gov (United States)

    Widrick, J. J.; Romatowski, J. G.; Karhanek, M.; Fitts, R. H.

    1997-01-01

    It is well known that skeletal muscle intrinsic maximal shortening velocity is inversely related to species body mass. However, there is uncertainty regarding the relationship between the contractile properties of muscle fibers obtained from commonly studied laboratory animals and those obtained from humans. In this study we determined the contractile properties of single chemically skinned fibers prepared from rat, rhesus monkey, and human soleus and gastrocnemius muscle samples under identical experimental conditions. All fibers used for analysis expressed type I myosin heavy chain as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Allometric coefficients for type I fibers from each muscle indicated that there was little change in peak tension (force/fiber cross-sectional area) across species. In contrast, both soleus and gastrocnemius type I fiber maximal unloaded shortening velocity (Vo), the y-intercept of the force-velocity relationship (Vmax), peak power per unit fiber length, and peak power normalized for fiber length and cross-sectional area were all inversely related to species body mass. The present allometric coefficients for soleus fiber Vo (-0.18) and Vmax (-0.11) are in good agreement with published values for soleus fibers obtained from common laboratory and domesticated mammals. Taken together, these observations suggest that the Vo of slow fibers from quadrupeds and humans scale similarly and can be described by the same quantitative relationships. These findings have implications in the design and interpretation of experiments, especially those that use small laboratory mammals as a model of human muscle function.

  1. [Contractile properties of fibers and cytoskeletal proteins of gerbil's hindlimb muscles after space flight].

    Science.gov (United States)

    Lipets, E N; Ponomareva, E V; Ogneva, I V; Vikhliantsev, I M; Karaduleva, E V; Kratashkina, N L; Kuznetsov, S L; Podlubnaia, Z A; Shenkman, B S

    2009-01-01

    The work had the goal to compare the microgravity effects on gerbil's muscles-antagonists, m. soleus and m. tibialis anterior. The animals were exposed in 12-d space microgravity aboard Earth's artificial satellite "Foton-M3". Findings of the analysis of single skinned fibers contractility are 19.7% diminution of the diameter and 21.8% loss of the total contractive force of m. soleus fibers post flight. However, there was no significant difference in calcium sensitivity which agrees with the absence of changes in the relative content of several major cytoskeletal proteins (titin and nebulin ratios to heavy chains of myosin were identical in the flight and control groups) and a slight shifting of the myosin phenotype toward the "fast type" (9%, p < 0.05). These parameters were mostly unaffected by the space flight in m. tibialis anterior. To sum up, the decline of contractility and diminution of gerbil's myofibers after the space flight were less significant as compared with rats and did not impact the sytoskeletal protein ratios. PMID:19711860

  2. The effects of cannabidiolic acid and cannabidiol on contractility of the gastrointestinal tract of Suncus murinus.

    Science.gov (United States)

    Cluny, Nina L; Naylor, Robert J; Whittle, Brian A; Javid, Farideh A

    2011-09-01

    Cannabidiol (CBD) has been shown to inhibit gastrointestinal (GI) transit in pathophysiologic in vivo models, while having no effect in physiologic controls. The actions of the precursor of CBD, cannabidiolic acid (CBDA), have not been investigated in the GI tract. The actions of these phytocannabinoids on the contractility of the GI tract of Suncus murinus were investigated in the current study. The effects of CBDA and CBD in resting state and pre-contracted isolated intestinal segments, and on the contractile effects of carbachol and electrical field stimulation (EFS) on the intestines of S. murinus were examined. CBDA and CBD induced a reduction in resting tissue tension of isolated intestinal segments which was not blocked by the cannabinoid CB1 receptor antagonist, AM251, the CB(2) receptor antagonist AM630, or tetrodotoxin. CBDA and CBD reduced the magnitude of contractions induced by carbachol and the tension of intestinal segments that were pre-contracted with potassium chloride. In tissues stimulated by EFS, CBDA inhibited contractions induced by lower frequencies (0.1-4.0 Hz) of EFS, while CBD inhibited contractions induced by higher frequencies (4.0-20.0 Hz) of EFS. The data suggest that CBDA and CBD have inhibitory actions on the intestines of S. murinus that are not neuronallymediated or mediated via CB(1) or CB(2) receptors. PMID:21975813

  3. A stepwise procedure to test contractility and susceptibility to injury for the rodent quadriceps muscle

    Directory of Open Access Journals (Sweden)

    Stephen J.P. Pratt

    2014-07-01

    Full Text Available In patients with muscle injury or muscle disease, assessment of muscle damage is typically limited to clinical signs, such as tenderness, strength, range of motion, and more recently, imaging studies.  Biological markers can also be used in measuring muscle injury, such as increased creatine kinase levels in the blood, but these are not always correlated with loss in muscle function (i.e. loss of force production.  This is even true of histological findings from animals, which provide a “direct measure” of damage, but do not account for loss of function.  The most comprehensive measure of the overall health of the muscle is contractile force.  To date, animal models testing contractile force have been limited to the muscle groups moving the ankle.  Here we describe an in vivo animal model for the quadriceps, with abilities to measure torque, produce a reliable muscle injury, and follow muscle recovery within the same animal over time.  We also describe a second model used for direct measurement of force from an isolated quadriceps muscle in situ. 

  4. Glucagon Increases Beating Rate but Not Contractility in Rat Right Atrium. Comparison with Isoproterenol.

    Directory of Open Access Journals (Sweden)

    Beatriz Merino

    Full Text Available This study evaluated the chronotropic and inotropic responses to glucagon in spontaneously beating isolated right atria of rat heart. For comparison, we also investigated the effects resulting from stimulating β-adrenoceptors with isoproterenol in this tissue. Isoproterenol increased both atrial frequency and contractility but glucagon only enhanced atrial rate. The transcript levels of glucagon receptors were about three times higher in sinoatrial node than in the atrial myocardium. Chronotropic responses to glucagon and isoproterenol were blunted by the funny current (If inhibitor ZD 7288. Inhibitors of protein kinase A, H-89 and KT-5720 reduced the chronotropic response to glucagon but not to isoproterenol. Inhibition of ryanodine receptors and calcium/calmodulin dependent protein kinase II (important regulators of sarcoplasmic reticulum Ca2+ release, with ruthenium red and KN-62 respectively, failed to alter chronotropic responses of either glucagon or isoproterenol. Non selective inhibition of phosphodiesterase (PDE with 3-isobutylmethylxantine or selective inhibition of PDE3 or PDE4 with cilostamide or rolipram respectively did not affect chronotropic effects of glucagon or isoproterenol. Our results indicate that glucagon increases beating rate but not contractility in rat right atria which could be a consequence of lower levels of glucagon receptors in atrial myocardium than in sinoatrial node. Chronotropic responses to glucagon or isoproterenol are mediated by If current but not by sarcoplasmic reticulum Ca2+ release, neither are regulated by PDE activity.

  5. Long-term vascular contractility assay using genipin-modified muscular thin films

    International Nuclear Information System (INIS)

    Vascular disease is a leading cause of death globally and typically manifests chronically due to long-term maladaptive arterial growth and remodeling. To date, there is no in vitro technique for studying vascular function over relevant disease time courses that both mimics in vivo-like tissue structure and provides a simple readout of tissue stress. We aimed to extend tissue viability in our muscular thin film contractility assay by modifying the polydimethylsiloxane (PDMS) substrate with micropatterned genipin, allowing extracellular matrix turnover without cell loss. To achieve this, we developed a microfluidic delivery system to pattern genipin and extracellular matrix proteins on PDMS prior to cell seeding. Tissues constructed using this method showed improved viability and maintenance of in vivo-like lamellar structure. Functional contractility of tissues fabricated on genipin-modified substrates remained consistent throughout two weeks in culture. These results suggest that muscular thin films with genipin-modified PDMS substrates are a viable method for conducting functional studies of arterial growth and remodeling in vascular diseases. (paper)

  6. Functional insights into modulation of BKCa channel activity to alter myometrial contractility

    Directory of Open Access Journals (Sweden)

    RamónALorca

    2014-07-01

    Full Text Available The large-conductance voltage- and Ca2+-activated K+ channel (BKCa is an important regulator of membrane excitability in a wide variety of cells and tissues. In myometrial smooth muscle, activation of BKCa plays essential roles in buffering contractility to maintain uterine quiescence during pregnancy and in the transition to a more contractile state at the onset of labor. Multiple mechanisms of modulation have been described to alter BKCa channel activity, expression, and cellular localization. In the myometrium, BKCa is regulated by alternative splicing, protein targeting to the plasma membrane, compartmentation in membrane microdomains, and posttranslational modifications. In addition, interaction with auxiliary proteins (i.e., β1- and β2-subunits, association with G-protein coupled receptor signaling pathways, such as those activated by adrenergic and oxytocin receptors, and hormonal regulation provide further mechanisms of variable modulation of BKCa channel function in myometrial smooth muscle. Here, we provide an overview of these mechanisms of BKCa channel modulation and provide a context for them in relation to myometrial function.

  7. Effect of exercise training and myocardial infarction on force development and contractile kinetics in isolated canine myocardium.

    Science.gov (United States)

    Canan, Benjamin D; Haizlip, Kaylan M; Xu, Ying; Monasky, Michelle M; Hiranandani, Nitisha; Milani-Nejad, Nima; Varian, Kenneth D; Slabaugh, Jessica L; Schultz, Eric J; Fedorov, Vadim V; Billman, George E; Janssen, Paul M L

    2016-04-15

    It is well known that moderate exercise training elicits a small increase in ventricular mass (i.e., a physiological hypertrophy) that has many beneficial effects on overall cardiac health. It is also well known that, when a myocardial infarction damages part of the heart, the remaining myocardium remodels to compensate for the loss of viable functioning myocardium. The effects of exercise training, myocardial infarction (MI), and their interaction on the contractile performance of the myocardium itself remain largely to be determined. The present study investigated the contractile properties and kinetics of right ventricular myocardium isolated from sedentary and exercise trained (10-12 wk progressively increasing treadmill running, begun 4 wk after MI induction) dogs with and without a left ventricular myocardial infarction. Exercise training increased force development, whereas MI decreased force development that was not improved by exercise training. Contractile kinetics were significantly slower in the trained dogs, whereas this impact of training was less or no longer present after MI. Length-dependent activation, both evaluated on contractile force and kinetics, was similar in all four groups. The control exercise-trained group exhibited a more positive force-frequency relationship compared with the sedentary control group while both sedentary and trained post-MI dogs had a more negative relationship. Last, the impact of the β-adrenergic receptor agonist isoproterenol resulted in a similar increase in force and acceleration of contractile kinetics in all groups. Thus, exercise training increased developed force but slowed contractile kinetics in control (noninfarcted animals), actions that were attenuated or completely absent in post-MI dogs. PMID:26823341

  8. Capacidades de fabricación y almacenaje óptimas en un sistema con logística inversa y demanda aleatoria

    OpenAIRE

    Benedito Benet, Ernest; Corominas Subias, Albert

    2009-01-01

    En el presente trabajo se estudia un sistema con demanda y retornos aleatorios para calcular las capacidades óptimas de fabricación y almacenaje. El modelo presentado permite estudiar el comportamiento de las capacidades de fabricación y almacenaje óptimas cuando varían los costes de producción.

  9. La medición de la capacidad de resolución de problemas en las ciencias experimentales

    Directory of Open Access Journals (Sweden)

    José Joaquín García García

    2012-01-01

    Full Text Available Este trabajo presenta un estudio, sobre el diseño de una prueba para medir la capacidad de resolución de problemas utilizando la técnica de análisis factorial. La prueba presentó un alfa de Cronbach (α de 0,76 y 16 peguntas distribuidas en 7 factores ordenados de acuerdo al porcentaje en el cual explicaban la varianza de la capacidad: predicción y transferencia, capacidad de síntesis, lectura crítica del enunciado, análisis, interpretación de información, comprensión metacognitiva de enunciados y procesos, delimitación del problema. Así, la resolución de problemas es una capacidad que está más relacionada con factores de carácter estructural y global, como la selección de soluciones e hipótesis más adecuadas y la representación misma del problema, y en menor medida con aspectos estratégicos de delimitación, precisión y planeación, tal vez por la usual ausencia de dichos aspectos en las aulas de clase.

  10. Efecto de los factores socioculturales en la capacidad de autocuidado del paciente hospitalizado con diabetes tipo 2

    Directory of Open Access Journals (Sweden)

    Mireya Gricelia Galindo-Martínez

    2014-01-01

    Full Text Available Introducción: la diabetes tipo 2 es considerada como un problema de salud pública por su creciente prevalencia e incidencia. Obje- tivo: describir el efecto de los factores socioculturales en la capacidad de autocuidado del paciente hospitalizado con diabetes tipo 2 en el Hospital General de Tecomán, Colima, México. Método: estudio transversal, observacional. Se trabajó con 68 pacientes hospitalizados con diabetes tipo 2; se utilizó un instrumento que midió el efecto de los factores socioculturales en la capacidad de autocuidado. El análisis de datos se realizó con Razón de Momios, intervalos de confianza al 95 % y fracción atribuible en expuestos. Resultados: los factores socioculturales tuvieron un leve efecto positivo en la capacidad de autocuidado (RM = 1,10; IC 95 %: 0,26-4,74 en pacientes hospitali- zados con diabetes tipo 2. Conclusiones: los factores socioculturales: experiencias vitales, experiencias laborales, religión, costumbres y tradiciones, prácticas curativas y ritos, señalados en la Teoría General del Déficit de Autocuidado, muestran un efecto positivo sobre la capacidad de autocuidado de las personas con diabetes tipo 2.

  11. La situación de las personas con capacidad intelectual límite en España

    Directory of Open Access Journals (Sweden)

    Agustín Huete García

    2016-06-01

    Full Text Available Se presentan los resultados principales de un estudio de carácter fundamentalmente descriptivo sobre la situación actual de las personas con capacidad intelectual límite en 2015. Se centra en describir la realidad demográfica y social de las personas con capacidad intelectual límite, sus necesidades de apoyo y extraer propuestas estratégicas hacia una inclusión social efectiva. Metodológicamente, de acuerdo al nivel de análisis, se optó por técnicas cuantitativas (análisis de fuentes estadísticas y cualitativas (entrevistas semiestructuradas a un grupo de personas con capacidad intelectual límite, familias y profesionales. Entre sus principales conclusiones, las personas con capacidad intelectual límite y sus familias demandan una visibilización de sus necesidades de apoyo para una inclusión social efectiva, siendo clave el apoyo para favorecer una vida autónoma e independiente. Las propuestas y buenas prácticas aportan elementos para un modelo de atención individualizada, al mismo tiempo fundamentada en una participación activa en la comunidad.

  12. CAP_USO: Um sistema especialista para determinação da capacidade de uso da terra

    Directory of Open Access Journals (Sweden)

    Monica Luri Giboshi

    1999-05-01

    Full Text Available O objetivo deste trabalho foi desenvolver um sistema especialista (CAP_USO para determinar a capacidade de uso da terra, visando auxiliar e dar mais agilidade ao processo de tomada de decisão para o planejamento do uso da terra. O sistema foi desenvolvido utilizando um software conhecido como C Language Integrated Production System (CLIPS, desenvolvido pela National Aeronautics and Space Administration (Nasa. Para determinar a capacidade de uso, foram considerados doze fatores limitantes ao uso da terra, além da declividade. A validação do sistema mostrou que o CAP_USO foi rigoroso ao determinar as classes de capacidade de uso por considerar a declividade em conjunto com os fatores que apresentam maior restrição ao uso da terra. A técnica de sistemas especialistas mostrou ser eficaz para a classificação de terras segundo a sua capacidade de uso, facilitando a manipulação e o processamento das informações necessárias à sua determinação.

  13. La relación entre la capacidad de atencion dividida y la estimación prospectiva del tiempo

    Directory of Open Access Journals (Sweden)

    Susana Celeste Azzollini

    2004-01-01

    Full Text Available Se realizó una investigación cuyo objetivo fue determinar si la capacidad de atención dividida resultaba explicativa de la variabilidad de las estimaciones prospectivas del tiempo. Los modelos teóricos sobre dicha estimación postulan que los recursos atencionales de procesamiento están divididos entre un procesador temporal, que cuenta y almacena unidades temporales subjetivas (Subjective Temporal Units - STUs y otras actividades de procesamiento no temporal (Zakay, 1993. Se seleccionó una muestra de 36 estudiantes universitarios que fueron asignados al azar a cada una de las seis condiciones experimentales, determinadas por un diseño factorial 3 x 2, y sus factores básicos fueron: cantidad de tareas y niveles de complejidad del material. Se confeccionaron materiales para las diferentes tareas y otros con el fin de evaluar las aptitudes mnémicas y la capacidad de atención dividida. Se solicitó a los sujetos que mientras realizaban las tareas, detuvieran un cronómetro cuando consideraran que había pasado un período de tiempo predeterminado. La estimación del tiempo se calculó por la diferencia en segundos entre el tiempo real y el tiempo estimado. Los datos se procesaron con análisis de correlación, discriminante y factorial de variancia. Los resultados obtenidos indican que la capacidad de atención dividida se correlaciona con la estimación temporal prospectiva y además, la capacidad de atención dividida resulta un buen predictor de la capacidad estimativa de los sujetos, especialmente de los sobre-estimadores. Las diferencias en las aptitudes mnémicas no explicaron la variabilidad de las estimaciones prospectivas.

  14. Capacidades e trajetórias de inovação de empresas brasileiras

    Directory of Open Access Journals (Sweden)

    Fernanda Maciel Reichert

    2015-10-01

    Full Text Available Todos os tipos de empresas têm condições e precisam inovar? Como se dá o processo de inovação? Quais são as características necessárias para inovar? O setor de atividade e o nível tecnológico influenciam? Para responder a esses questionamentos é preciso ir além do conceito de inovação como a simples criação de algo novo. É preciso considera-la, principalmente, como uma iniciativa de mudança para preencher lacunas de conhecimento e de mercado, e assim, gerar resultados para as empresas. Sabe-se que todas as empresas sempre têm uma base tecnológica (i.e. um produto e seu processo como objeto de seus negócios (a saber, a gestão interna e as transações externas. E, por isso, sempre são constituídas, em menor ou maior grau, por quatro funções básicas - desenvolvimento, operação, gestão e comercialização. A inovação emerge justamente dessas funções e, para cada uma delas, corresponderá uma capacidade de inovação. Assim, o objetivo do presente artigo é identificar as capacidades de inovação de empresas industriais brasileiras e, com isso, explicitar suas trajetórias de inovação. Para realizar o objetivo proposto, utilizou-se como base de dados os resultados oriundos de projeto de pesquisa uma amostra de 1326 empresas industriais brasileiras. O projeto desenvolveu-se em três fases: (i desenvolvimento de um modelo teórico de capacidades de inovação da firma; (ii fase exploratória e; (iii levantamento de dados (survey junto às empresas de setores industriais do estado do Rio Grande do Sul. Considerando o perfil da amostra, concluiu-se que a empresa típica é uma prestadora de serviços industriais, com baixo potencial de inovação. Seja por conta dos ramos de atividade, em sua maioria de baixa e média baixa intensidade tecnológica, seja pela predominância de um modelo de gestão familiar focado em custos, sua trajetória de inovação é restrita à manutenção da qualidade e à maximização da

  15. INFLUÊNCIA DO TIRO COM ZARABATANA NA CAPACIDADE RESPIRATÓRIA DO IDOSO

    Directory of Open Access Journals (Sweden)

    Catarina Leitão

    2015-05-01

    Full Text Available A  respiração é um processo vital e faz parte das funções primordiais do corpo humano. A falta de consciência respiratória é responsável por diversos problemas o que leva a que com o passar do tempo, a respiração, possa provocar não só deformidades no tórax como também modificações posturais, incapacidade funcional e diversas patologias (Tribastone, 2001.O sistema respiratório é o sistema do organismo que envelhece mais rápido devido à exposição aos poluentes ambientais ao longo da vida. A nível clínico ocorrem mudanças relevantes que se traduzem na deterioração da função pulmonar. Calculando-se que em Portugal, as doenças respiratórias a curto prazo sejam a terceira causa de morte e afetem maioritariamente as pessoas idosas (Ruivo et al., 2009. No processo respiratório são essenciais dois tempos, a inspiração e expiração, que resultam de movimentos respiratórios nos quais o diafragma e os pulmões exercem funções fundamentais (Parreiral, 2005. Acreditamos que o tiro com zarabatana, que trabalha a expiração- através da expulsão enérgica de dardos e a inspiração- aquando do início do treino, poderá ser benéfico para a capacidade respiratória dos idosos. Objetivo: O presente estudo visa avaliar os efeitos da prática de Tiro com Zarabatana, na capacidade respiratória de pessoas idosas, mais precisamente as alterações ao nível do débito expiratório máximo.

  16. La arquitectura organizacional y las capacidades estratégicas empresariales empresariales

    Directory of Open Access Journals (Sweden)

    Jesús Perdomo-Ortiz

    2016-05-01

    Full Text Available El diseño organizacional y la teoría basada en los recursos han sido las dos áreas de la economía de la empresa que más desarrollo e impacto en la gerencia, y en el estado del arte de la disciplina, han tenido durante los últimos 15 años. Este documento aborda desde una perspectiva comparada ambos temas y encuentra una estrecha relación de mutuo apoyo conceptual. Es importante precisar que el presente documento no es una revisión de la literatura sobre diseño o arquitectura organizacional; no obstante se soporta en un conjunto de lecturas centradas en dicha temática. En este sentido, el objetivo del documento es hacer una reflexión crítica, planteando unas hipótesis de trabajo y argumentando su elaboración. La literatura sobre dirección estratégica tiene como uno de sus soportes disciplinares la denominada teoría (visión de la empresa basada en los recursos (The Resource-Based View of the Firm, RBV y defiende una lógica implícita de causalidad, al considerar la estructura organizacional como una consecuencia de la estrategia empresarial. Una visión crítica de la arquitectura organizacional contrasta la causalidad estrategia → estructura y puede explicar un vacío en la RBV, cuando ésta trata de explicar el tránsito del concepto de recurso a capacidad, haciendo alusión a un necesario proceso de integración de recursos básicos. Este documento plantea, entonces, la hipótesis de que la arquitectura organizacional es una forma de explicar la conformación de capacidades empresariales y, desde su argumentación, muestra cómo la causalidad → estrategia estructura se puede entender en forma inversa.

  17. POTÊNCIA E CAPACIDADE ANAERÓBIA EM ATLETAS DE KARATE

    Directory of Open Access Journals (Sweden)

    Keith Sato Urbinati

    2011-01-01

    Full Text Available O objetivo do presente estudo foi verificar o nível de potência muscular, bem como a intensidade de esforço durante prova de potência e capacidade anaeróbia em atletas de Karate. Participaram do estudo treze karatekas especialistas em prova de kumitê (luta,sexo masculino, idade média de 20,7±3,79 anos. Realizou-se a avaliação de potência e capacidade anaeróbia (Wingate test, com coleta das variáveis fisiológicas:frequência cardíaca (FC, pressão arterial (PA e lactato sanguíneo (LAC de repouso, imediatamente após e em 5 min de recuperação. Para verificar se ocorrem diferenças nas variáveis fisiológicas durante o teste, realizou-se análise de variância, post hoc de Bonferronie (p?0,05. Verificou-se potência de pico(PP de 8,77 ± 0,99 W/kg-1, potência média (PM de 5,13± 0,49 W.kg-1 e % de fadiga de 50,5 ± 6,45%. Para as variáveis fisiológicas,a FC (F=9,79; p=0,00 pós-teste de Wingate foi estatisticamente maior e diferenteda FC de repouso e recuperação. Não foram encontradas diferenças nos níveis pressatórios e nas concentrações de LAC. Em relação aos valores de FC e LAC tais variáveis pós-teste não se apresentaram elevados. Os valores de potência anaeróbia estão de acordo com a necessidade da modalidade esportiva. Assim, verificou-se menores intensidades de esforço em teste de Wingate quando comparados a de situação real de competição. O nível de potência muscular parece estar adequado ao kumitê.

  18. Effect of acute and chronic simvastatin treatment on post-ischemic contractile dysfunction in isolated rat heart

    Czech Academy of Sciences Publication Activity Database

    Szárszoi, Ondrej; Malý, J.; Ošťádal, P.; Netuka, I.; Bešík, J.; Kolář, František; Ošťádal, Bohuslav

    2008-01-01

    Roč. 57, č. 5 (2008), s. 793-796. ISSN 0862-8408 R&D Projects: GA MŠk(CZ) 1M0510 Institutional research plan: CEZ:AV0Z50110509 Keywords : simvastatin * contractile dysfunction * protection Subject RIV: FA - Cardiovascular Diseases incl. Cardiotharic Surgery Impact factor: 1.653, year: 2008

  19. Effects of dibutyryl cyclic adenosine monophosphate on hypercapnic depression of diaphragmatic contractility in pentobarbital-anesthetized dogs

    OpenAIRE

    FUJII, YOSHITAKA; Uemura, Aki

    2010-01-01

    Background: Hypercapnia is associated with diaphragm muscle dysfunction that causes a reduction of diaphragmatic force generated for a constant elective myographic activity. No published data are available concerning hypercapnic depression of diaphragmatic contractility during dibutyryl cyclic adenosine monophospate (DBcAMP) administration.

  20. The Effects of Anticholinesterase Drugs on Bethanechol-İnduced Contractile Responses in Rat Ileum Smooth Muscle

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    Ilker Kelle

    2007-01-01

    Full Text Available At present, anticholinesterase drugs are valuable as therapeutic agents when cholinergic activity decreased in cases such as glokom, myastenia gravis and postoperative period which induces atony in bladder and intestinal smooth muscles. In these conditions anticholinesterases are expected to increase the levels of acetylcholine by inhibiting the enzyme acetylcholinesterase, thus they can exhibit both muscarinic and nicotinic effects indirectly rather than constituting a direct effect on muscarinic receptors. But recently, it has been shown that anticholinesterase drugs may exhibit a dose-dependent antimuscarinic effect by interacting with muscarinic receptors directly. According to these results, we investigated the dose-dependent effects of anticholinesterases on bethanechol-induced contractile responses at ileum smooth muscle preparations. Our results show that none of the three anticholinesterases did cause any potentialization on contractile responses for bethanechol at their first three concentrations (p > 0,05; paired samples t test. But at their highest concentration such as 1000 M, neostigmine, edrophonium and piridostigmine attenuated the bethanechol induced contractile responses (p 0,05; paired samples t test. These results suggest that anticholinesterases do not have dual effects on bethanechol induced contractile responses in rat ileum smooth muscle preparations. Larger doses of anticholinesterases may evoke antimuscarinic effects on ileum smooth muscle contractions.

  1. The Effect of Cleft Palate Repair on Contractile Properties of Single Permeabilized Muscle Fibers From Congenitally Cleft Goats Palates

    Science.gov (United States)

    A cleft palate goat model was used to study the contractile properties of the levator veli palatini (LVP) muscle which is responsible for the movement of the soft palate. In 15-25% of patients that undergo palatoplasty, residual velopharyngeal insufficiency (VPI) remains a problem and often require...

  2. Neuropeptide Y, its localization in the human cervix and possible effect on the contractile activity of cervix smooth muscle.

    Science.gov (United States)

    Norström, A; Bryman, I; Dahlström, A

    1992-01-01

    Immunochemical methods were used to identify neuropeptide Y (NPY) in the cervical tissue of women at early and term pregnancy. NPY-containing fibers could not be demonstrated in the upper and lower uterine segments at term, but the cervical innervation persisted during labor. Moreover, NPY alone did not affect cervical contractile activity, although the stimulatory effect of noradrenaline was enhanced. PMID:1427420

  3. Effects of silver ions (Ag+) on contractile ring function and microtubule dynamics during first cleavage in Ilyanassa obsoleta

    Science.gov (United States)

    Conrad, A. H.; Stephens, A. P.; Paulsen, A. Q.; Schwarting, S. S.; Conrad, G. W.; Spooner, B. S. (Principal Investigator)

    1994-01-01

    The terminal phase of cell division involves tight constriction of the cleavage furrow contractile ring, stabilization/elongation of the intercellular bridge, and final separation of the daughter cells. At first cleavage, the fertilized eggs of the mollusk, Ilyanassa obsoleta, form two contractile rings at right angles to each other in the same cytoplasm that constrict to tight necks and partition the egg into a trefoil shape. The cleavage furrow contractile ring (CF) normally constricts around many midbody microtubules (MTs) and results in cleavage; the polar lobe constriction contractile ring (PLC) normally constricts around very few MTs and subsequently relaxes without cleavage. In the presence of Ag+ ions, the PLC 1) begins MT-dependent rapid constriction sooner than controls, 2) encircles more MTs than control egg PLCs, 3) elongates much more than control PLCs, and 4) remains tightly constricted and effectively cleaves the polar lobe from the egg. If Ag(+)-incubated eggs are returned to normal seawater at trefoil, tubulin fluorescence disappears from the PLC neck and the neck relaxes. If nocodazole, a drug that depolymerizes MTs, is added to Ag(+)-incubated eggs during early PLC constriction, the PLC is not stabilized and eventually relaxes. However, if nocodazole is added to Ag(+)-incubated eggs at trefoil, tubulin fluorescence disappears from the PLC neck but the neck remains constricted. These results suggest that Ag+ accelerates and gradually stabilizes the PLC constriction by a mechanism that is initially MT-dependent, but that progressively becomes MT-independent.

  4. Myocardial performance index is sensitive to changes in cardiac contractility, but is also affected by vascular load condition.

    Science.gov (United States)

    Uemura, Kazunori; Kawada, Toru; Zheng, Can; Li, Meihua; Shishido, Toshiaki; Sugimachi, Masaru

    2013-01-01

    Myocardial performance index (MPI), or Tei index, is measured by Doppler echocardiography in clinical practice. MPI has been shown to be useful in evaluating left ventricular (LV) performance and predicting prognosis in cardiac patients. However, the effects of LV load and contractile states on MPI remain to be thoroughly investigated. In 14 anesthetized dogs, we obtained LV pressure-volume relationship with use of sonomicrometry and catheter-tip manometry. MPI was determined from the time derivative of LV volume and pressure. LV end-systolic pressure-volume ratio (Ees'), effective arterial elastance (Ea) and LV end-diastolic volume (Ved) were used as indices of LV contractility, afterload and preload, respectively. Hemodynamic conditions were varied over wide ranges [heart rate (HR), 66-192 bpm; mean arterial pressure, 71-177 mmHg] by infusing cardiovascular agents, by inducing ischemic heart failure and by electrical atrial pacing. Multiple linear regression analysis of pooled data (66 data sets) indicated that MPI (0.6-1.8) significantly correlated with Ees' [1.5-17.5 mmHg · ml(-1), pVed (11-100 ml, p0.1). Theoretical analysis also indicated that MPI decreases following the increases in LV contractility and in preload, while it increases in response to an increase in LV afterload. We conclude that MPI sensitively detects changes in LV contractility. However, MPI is also affected by changes in LV afterload and preload. PMID:24109782

  5. Decrease of contractile properties and transversal stiffness of single fibers in human soleus after 7-day “dry” immersion

    Science.gov (United States)

    Ogneva, I. V.; Ponomareva, E. V.; Kartashkina, N. L.; Altaeva, E. G.; Fokina, N. M.; Kurushin, V. A.; Kozlovskaya, I. B.; Shenkman, B. S.

    2011-05-01

    The simulation model of "dry" immersion was used to evaluate the effects of plantar mechanical stimulation (PMS) and high frequency electromyostimulation (EMS) on the mechanical properties of human soleus fibers under the conditions of gravitational unloading. We examined contractile properties of single fibers by means of tensometry, transversal stiffness of sarcolemma and different areas of the contractile apparatus by means of atomic force microscopy. It was shown that there is a reduction of transversal stiffness in single muscle fibers under hypogravitational conditions. Application of different countermeasures could compensate this effect. Meanwhile pneumostimulation and electro stimulation act in quite different way. Therefore, pneumostimulation seems to be more effective. The data obtained can be considered as the evidence of the fact that such countermeasures as PMS and electromyostimulation influence on muscle fibers in quite different ways and PMS efficiency is likely to be higher. On the basis of our experimental data on transverse stiffness of mechanotransductional nodes and the contractile apparatus, we can assume that support stimulation allows prevention of destructive processes in muscle fibers. Electrostimulation seems to stimulate contractile activity only without suppression of impairment of the fiber mechanical properties.

  6. Effect of a Periodized Power Training Program on the Functional Performances and Contractile Properties of the Quadriceps in Sprinters

    Science.gov (United States)

    Kamandulis, Sigitas; Skurvydas, Albertas; Brazaitis, Marius; Stanislovaitis, Aleksas; Duchateau, Jacques; Stanislovaitiene, Jurate

    2012-01-01

    Our purpose was to compare the effect of a periodized preparation consisting of power endurance training and high-intensity power training on the contractile properties of the quadriceps muscle and functional performances in well trained male sprinters (n = 7). After 4 weeks of high-intensity power training, 60-m sprint running time improved by an…

  7. Effect of subarachnoid hemorrhage on contractile responses and noradrenaline release evoked in cat cerebral arteries by histamine

    International Nuclear Information System (INIS)

    This study analyzes the changes induced by subarachnoid hemorrhage (SAH) on the contractile responses and the noradrenaline release evoked in cat cerebral arteries by histamine. The dose-dependent vasoconstriction induced by histamine on the cerebral arteries of normal cats was significantly reduced by diphenhydramine and phentolamine. When SAH was produced 3 and 7 days before the experiment, the histamine-induced vasoconstriction also decreased. Thereafter, a tendency to normalization in the contractile vascular responses was observed such that in 15 days after the hemorrhage it was not significantly different from that found in controls animals. The decrease in the contractile responses to histamine provoked by SAH was similar to that seen after pretreatment with intracisternal injections of 6-hydroxydopamine. The amount of radioactivity released by histamine following preincubation with 3H-noradrenaline from the cerebral arteries of cats exposed to SAH 3, 7, and 15 days before the experiment was significantly reduced when compared with controls. Moreover, the basal level of tritium release and the radioactivity retained at the end of the experiment were also decreased after SAH. Results indicate histamine releases noradrenaline from cat cerebral arteries, and SAH produce a transient denervation of the perivascular adrenergic nerve endings, which explained by the impairment of the indirect adrenergic mechanism involved in the overall contractile response elicited by this amine in cerebral arteries. Histamine does not seem to play a significant role in the production of the cerebral vasospasm occurring after SAH

  8. Ultrastructural analysis of the contractile apparatus in the rat’s heart on the stages of ontogeny

    Directory of Open Access Journals (Sweden)

    Kozlov S.V.

    2015-03-01

    Full Text Available Background. Cardiovascular disease is a major cause of human mortality and disability at working age. Many disorders of cardiac sarcomeres components have been linked to human cardiomyopathies, including desminopathy, dilated cardiomyopathy, hypertrophic cardiomyopathy, familial atrial septal defects, and others. Objective. The aim of this work was to determine the remodeling of the contractile apparatus in cardiomyocytes in rat ventricular myocardium on the stages of ontogeny. Methods. White rats were used as a material. Hearts were investigated by the transmission electron microscopy during the stages of prenatal and postnatal ontogeny. Results. Active myofibrilogenesis observed at 18th day of development and dominated in the right ventricle of myocardium during fetal development. After birth there was alignment of myofibrils content in the myocardium of both ventricles. Rapid growth of myofibrils content was seen in the sarcoplasm of cardiomyocytes, which took priority of development in the left ventricle during the period from the 3rd to the 14th day of postnatal ontogeny. At 30th day after the birth the contractile apparatus in the rat’s ventricular myocardium acquired signs of maturity. Conclusion. Development of the contractile apparatus in the rat’s ventricular myocardium is nonlinear and depends on the functional load of heart chamber. Citation: Kozlov SV, Mayevsky AE, Mіshalov VD, Sulayeva ON. [Ultrastructural analysis of the contractile apparatus in the rat’s heart on the stages of ontogeny]. Morphologia. 2015;9(1:34-8. Ukrainian.

  9. Cardamonin inhibits agonist-induced vascular contractility via Rho-kinase and MEK inhibition.

    Science.gov (United States)

    Je, Hyun Dong; Jeong, Ji Hoon

    2016-01-01

    The present study was undertaken to investigate the influence of cardamonin on vascular smooth muscle contractility and to determine the mechanism(s) involved. Denuded aortic rings from male rats were used and isometric contractions were recorded and combined with molecular experiments. Cardamonin significantly relaxed fluoride-, phenylephrine-, and phorbol ester-induced vascular contractions, suggesting that it has an anti-hypertensive effect on agonist-induced vascular contraction regardless of endothelial nitric oxide synthesis. Furthermore, cardamonin significantly inhibited the fluoride-induced increase in pMYPT1 level and phenylephrine-induced increase in pERK1/2 level, suggesting inhibition of Rho-kinase and MEK activity and subsequent phosphorylation of MYPT1 and ERK1/2. This study provides evidence that the relaxing effect of cardamonin on agonist-induced vascular contraction regardless of endothelial function involves inhibition of Rho-kinase and MEK activity. PMID:26807025

  10. Bidirectional Interplay between Vimentin Intermediate Filaments and Contractile Actin Stress Fibers

    Directory of Open Access Journals (Sweden)

    Yaming Jiu

    2015-06-01

    Full Text Available The actin cytoskeleton and cytoplasmic intermediate filaments contribute to cell migration and morphogenesis, but the interplay between these two central cytoskeletal elements has remained elusive. Here, we find that specific actin stress fiber structures, transverse arcs, interact with vimentin intermediate filaments and promote their retrograde flow. Consequently, myosin-II-containing arcs are important for perinuclear localization of the vimentin network in cells. The vimentin network reciprocally restricts retrograde movement of arcs and hence controls the width of flat lamellum at the leading edge of the cell. Depletion of plectin recapitulates the vimentin organization phenotype of arc-deficient cells without affecting the integrity of vimentin filaments or stress fibers, demonstrating that this cytoskeletal cross-linker is required for productive interactions between vimentin and arcs. Collectively, our results reveal that plectin-mediated interplay between contractile actomyosin arcs and vimentin intermediate filaments controls the localization and dynamics of these two cytoskeletal systems and is consequently important for cell morphogenesis.

  11. A new strain gage method for measuring the contractile strain ratio of Zircaloy tubing

    International Nuclear Information System (INIS)

    An improved strain gage method for determining the contractile strain ratio (CSR) of Zircaloy tubing was developed. The new method consists of a number of load-unload cyclings at approximately 0.2% plastic strain interval. With this method the CSR of Zircaloy-4 tubing could be determined accurately because it was possible to separate the plastic strains from the elastic strain involvement. The CSR values determined by use of the new method were in good agreement with those calculated from conventional post-test manual measurements. The CSR of the tubing was found to decrease with the amount of deformation during testing because of uneven plastic flow in the gage section. A new technique of inscribing gage marks by use of a YAG laser is discussed. (orig.)

  12. Tuning cell migration: contractility as an integrator of intracellular signals from multiple cues.

    Science.gov (United States)

    Bordeleau, Francois; Reinhart-King, Cynthia A

    2016-01-01

    There has been immense progress in our understanding of the factors driving cell migration in both two-dimensional and three-dimensional microenvironments over the years. However, it is becoming increasingly evident that even though most cells share many of the same signaling molecules, they rarely respond in the same way to migration cues. To add to the complexity, cells are generally exposed to multiple cues simultaneously, in the form of growth factors and/or physical cues from the matrix. Understanding the mechanisms that modulate the intracellular signals triggered by multiple cues remains a challenge. Here, we will focus on the molecular mechanism involved in modulating cell migration, with a specific focus on how cell contractility can mediate the crosstalk between signaling initiated at cell-matrix adhesions and growth factor receptors. PMID:27508074

  13. Contractile responses to ergotamine and dihydroergotamine in the perfused middle cerebral artery of rat

    DEFF Research Database (Denmark)

    Tfelt-Hansen, Peer; Nilsson, Elisabeth; Edvinsson, Lars

    2007-01-01

    The vasomotor effects of ergotamine and dihydroergotamine (DHE) on the middle cerebral artery (MCA) of rats were studied using the pressurised arteriography method and in vitro myographs. MCAs from Sprague-Dawley rats were mounted on two glass micropipettes using the arteriograph, pressurised to 85...... mmHg and luminally perfused. All vessels used attained spontaneous contractile tone (34.9+/-1.8% of resting tone) and responded to luminal adenosine triphosphate (ATP) with dilatation (24.1+/-4.0%), which showed functioning endothelium. Luminally added ergotamine or DHE induced maximal contractions...... of 16.8+8% and 22.4+/-0.9%, respectively, compared to the resting diameter, with a pEC(50) of 8.7+/-0.1 for ergotamine and 9.0+/-0.1 for DHE. Abluminal application of ergotamine and DHE also caused concentration-dependent contractions of the perfused MCA by 21.4+/-2.1% and 23.1+/-7.0%, respectively...

  14. [Contractile properties of skeletal muscles of rats after flight on "Kosmos-1887"].

    Science.gov (United States)

    Oganov, V S; Skuratova, S A; Murashko, L M

    1991-01-01

    Contractile properties of skeletal muscles of rats were investigated using glycerinated muscle preparations that were obtained from Cosmos-1887 animals flown for 13 days (plus 2 days on the ground) and from rats that remained hypokinetic for 13 days on the ground. In the flow rats, the absolute mass of postural muscles remained unchanged while their relative mass increased; this may be attributed to their enhanced hydration which developed during the first 2 days after landing. Strength losses of the postural muscles were less significant than after previous flights. Comparison of the Cosmos-1887 and hypokinesia control data has shown that even 2-day exposure to 1 G after 13-day flight can modify drastically flight-induced changes. PMID:1870316

  15. Disordered actomyosin networks are sufficient to produce cooperative and telescopic contractility

    Science.gov (United States)

    Linsmeier, Ian; Banerjee, Shiladitya; Oakes, Patrick W.; Jung, Wonyeong; Kim, Taeyoon; Murrell, Michael P.

    2016-01-01

    While the molecular interactions between individual myosin motors and F-actin are well established, the relationship between F-actin organization and actomyosin forces remains poorly understood. Here we explore the accumulation of myosin-induced stresses within a two-dimensional biomimetic model of the disordered actomyosin cytoskeleton, where myosin activity is controlled spatiotemporally using light. By controlling the geometry and the duration of myosin activation, we show that contraction of disordered actin networks is highly cooperative, telescopic with the activation size, and capable of generating non-uniform patterns of mechanical stress. We quantitatively reproduce these collective biomimetic properties using an isotropic active gel model of the actomyosin cytoskeleton, and explore the physical origins of telescopic contractility in disordered networks using agent-based simulations. PMID:27558758

  16. Prostaglandins attenuate cardiac contractile dysfunction produced by free radical generation but not by hydrogen peroxide.

    Science.gov (United States)

    Zimmer, K M; Karmazyn, M

    1997-11-01

    The aim of this study was to examine and compare the potential influence of cyclooxygenase or lipoxygenase derived metabolites of arachidonic acid on myocardial injury produced either by a free radical generating system consisting of purine plus xanthine oxidase or that produced by hydrogen peroxide. A free radical generating system consisting of purine (2.3 mM) and xanthine oxidase (10 U/L) as well as hydrogen peroxide (75 microM) produced significant functional changes in the absence of either significant deficits in high energy phosphates or ultrastructural damage. Prostaglandin F2 alpha (30 nM) significantly attenuated both the negative inotropic effect of purine plus xanthine oxidase as well as the ability of the free radical generator to elevate diastolic pressure. An identical concentration of prostaglandin 12 (prostacyclin) significantly reduced diastolic pressure elevation only and had no effect on contractile depression. The salutary effects of the two PGs occurred in the absence of any inhibitory influence on superoxide anion generation produced by the purine and xanthine oxidase reaction. None of prostaglandins modulated the response to hydrogen peroxide. In addition, neither prostaglandin E2 nor leukotrienes exerted any effect on changes produced by either type of oxidative stress. A 5 fold elevation in the concentrations of free radical generators or hydrogen peroxide produced extensive injury as characterized by a virtual total loss in contractility, 400% elevation in diastolic pressure, ultrastructural damage and significant depletions in high energy phosphate content. None of these effects were modulated by eicosanoid treatment. Our results therefore demonstrate a selective ability of both prostaglandin F2 alpha and to a lesser extent prostacyclin, to attenuate dysfunction produced by purine plus xanthine oxidase but not hydrogen peroxide. It is possible that these eicosanoids may represent endogenous protective factors under conditions of enhanced

  17. Effects of benactyzine on action potentials and contractile force of guinea pig papillary muscles

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Aim:To explore the effects of benactyzine (BEN) on the action potential and contractile force in guinea pig papillary muscles.Methods:Conventional microelectrode technique was used to record the fast action potentials (FAP) and slow action potentials (SAP) of guinea pig papillary muscles.Results:Benactyzine 5,10,50 μmol·L-1 suppressed the maximal upstroke velocity (vmax) of FAP and contractile force (Fc) concentration-dependently while prolonged the action potential duration at 50%,90% repolarization (APD50,APD90) and effective refractory period (ERP) of FAP.The suppression on the vmax was frequency-dependent.Benactyzine 5,10,50μmol·L-1 lengthened the APD50,APD90 of SAP induced by isoprenaline or histamine when perfused with KCl 22 mmol·L-1 Tyrode's solution.The vmax of the SAP was not decreased by benactyzine 5,10 μmol·L-1 but by 50 μmol·L-1.The effects on the SAP were antagonized by elevation of the extracellular calcium from 2.0 to 5.6 mmol·L-1.The effects of benactyzine on SAP elicited by tetrodotoxin resembled that by isoprenaline or histamine except the more pronounced suppression on vmax and action potential amplitude (APA).The persistent rapid spontaneous activity and triggered tachyarrhythmia induced by ouabain were also abolished immediately by benactyzine 5 μmol·L-1.Conclusion:Benactyzine can inhibit Na+,K+,Ca2+ transmembrane movement and intracellular Ca2+ mobilization in the myocardium,and this may be the electrophysiological basis of its effects against experimental arrhythmias.

  18. Troglitazone stimulates β-arrestin-dependent cardiomyocyte contractility via the angiotensin II type 1A receptor

    International Nuclear Information System (INIS)

    Peroxisome proliferator-activated receptor γ (PPARγ) agonists are commonly used to treat cardiovascular diseases, and are reported to have several effects on cardiovascular function that may be due to PPARγ-independent signaling events. Select angiotensin receptor blockers (ARBs) interact with and modulate PPARγ activity, thus we hypothesized that a PPARγ agonist may exert physiologic effects via the angiotensin II type 1A receptor (AT1AR). In AT1AR-overexpressing HEK 293 cells, both angiotensin II (Ang II) and the PPARγ agonist troglitazone (Trog) enhanced AT1AR internalization and recruitment of endogenous β-arrestin1/2 (βarr1/2) to the AT1AR. A fluorescence assay to measure diacylglycerol (DAG) accumulation showed that although Ang II induced AT1AR-Gq protein-mediated DAG accumulation, Trog had no impact on DAG generation. Trog-mediated recruitment of βarr1/2 was selective to AT1AR as the response was prevented by an ARB- and Trog-mediated βarr1/2 recruitment to β1-adrenergic receptor (β1AR) was not observed. In isolated mouse cardiomyocytes, Trog increased both % and rate of cell shortening to a similar extent as Ang II, effects which were blocked with an ARB. Additionally, these effects were found to be βarr2-dependent, as cardiomyocytes isolated from βarr2-KO mice showed blunted contractile responses to Trog. These findings show for the first time that the PPARγ agonist Trog acts at the AT1AR to simultaneously block Gq protein activation and induce the recruitment of βarr1/2, which leads to an increase in cardiomyocyte contractility.

  19. Relaxation of evoked contractile activity of isolated guinea-pig ileum by (+/-)-kavain.

    Science.gov (United States)

    Seitz, U; Ameri, A; Pelzer, H; Gleitz, J; Peters, T

    1997-08-01

    Kava pyrones are the pharmacologically active compounds of Piper methysticum Forst. In the present study, the effect of the synthetic kava pyrone (+/-)-kavain was investigated on evoked contractile activity of isolated guinea-pig ileum. (+/-)-Kavain (1 microM-1 mM) dose-dependently reduced contractions of ileum evoked by carbachol (10 microM), by BAY K 8644 (0.3 microM), or by substance P (0.05 microM). (+/-)-Kavain also inhibited the contractile responses induced by raising the extracellular K+ concentration from 4 to 20 mM and by blocking the K+ channel by barium chloride (1 mM) or 4-aminopyridine (0.3 mM). After pre-incubation with 1 microM nifedipine, carbachol (1 microM) evoked 18.2 +/- 14.3% of contraction at control (i.e. prior pre-incubation with nifedipine). This remaining response was completely abolished by high concentrations of (+/-)-kavain (400 microM). After treatment of the longitudinal ileum strips with pertussis toxin (PTX), carbachol (1 microM) evoked 27.0 +/- 6.2% of the control response in untreated ileum. These contractions were also blocked by (+/-)-kavain (400 microM). However, (+/-)-kavain had no effect on the caffeine-induced (20 mM) contractions of ileum strips, which were permeabilized with digitonin or beta-escin. Moreover, it failed to affect Ca(2+)-evoked contractions of skinned muscles. These results suggest that the kava pyrone (+/-)-kavain may act in a non-specific musculotropic way on the smooth muscle membrane. PMID:9270372

  20. Improved Cardiac Contractility of Human Recombinant Growth Hormone on the Congestive Heart Failure of Pig

    Institute of Scientific and Technical Information of China (English)

    Yang Ping; He Yu-quan; Zeng Hong; Ni Jin-song; Yun Qing-jun; Huang Xiao-ping; Li Shu-mei

    2005-01-01

    The enhanced cardiac contractility effect of human recombinant growth hormone (hr-GH) on the congestive heart failure (CHF) was studied on the pig. To build a pig model of congestive heart failure, a temporary artificial cardiac pacemaker was implanted in the pig's body and paced at 220 beats to 240 beats per minute for 1 week. After the model of congestive heart failure was successfully set up, the frequency of the pacemaker was changed to 150 beats to 180 beats per minute to maintain the CHF model stable. Pigs were divided into three groups: The hr-GH group in which 0.5 mg/kg per day of hr-GH was administrated intramuscularly for 15 days, the injection control group in which an equal amount of physiological saline was injected intramuscularly, and a normal control group. The left ventricular diastolic end pressure was (10.60±2.41 ) mmHg in the hr-GH group, but (19.00±3.81) mmHg in the saline control group (P<0.01); Cardiac output was (1.86±0.13) L/min in the hr-GH group, but (1.56 ±0.18) L/min in the saline control group (P<0.05); Peripheral min) -1 in the saline control group (P<0.05); ± dp/dtmax was (2900 ±316.23) and (2280 ±286.36) in the hr-HG group and the saline control group respectively (P<0.05). The results show that hr-GH enhances myocardial contractility of CHF, and the CHF model built by a temporary artificial cardiac pacemaker at a high rate of stimulation is reasonable and applicable.

  1. Pentamidine rescues contractility and rhythmicity in a Drosophila model of myotonic dystrophy heart dysfunction

    Directory of Open Access Journals (Sweden)

    Mouli Chakraborty

    2015-12-01

    Full Text Available Up to 80% of individuals with myotonic dystrophy type 1 (DM1 will develop cardiac abnormalities at some point during the progression of their disease, the most common of which is heart blockage of varying degrees. Such blockage is characterized by conduction defects and supraventricular and ventricular tachycardia, and carries a high risk of sudden cardiac death. Despite its importance, very few animal model studies have focused on the heart dysfunction in DM1. Here, we describe the characterization of the heart phenotype in a Drosophila model expressing pure expanded CUG repeats under the control of the cardiomyocyte-specific driver GMH5-Gal4. Morphologically, expression of 250 CUG repeats caused abnormalities in the parallel alignment of the spiral myofibrils in dissected fly hearts, as revealed by phalloidin staining. Moreover, combined immunofluorescence and in situ hybridization of Muscleblind and CUG repeats, respectively, confirmed detectable ribonuclear foci and Muscleblind sequestration, characteristic features of DM1, exclusively in flies expressing the expanded CTG repeats. Similarly to what has been reported in humans with DM1, heart-specific expression of toxic RNA resulted in reduced survival, increased arrhythmia, altered diastolic and systolic function, reduced heart tube diameters and reduced contractility in the model flies. As a proof of concept that the fly heart model can be used for in vivo testing of promising therapeutic compounds, we fed flies with pentamidine, a compound previously described to improve DM1 phenotypes. Pentamidine not only released Muscleblind from the CUG RNA repeats and reduced ribonuclear formation in the Drosophila heart, but also rescued heart arrhythmicity and contractility, and improved fly survival in animals expressing 250 CUG repeats.

  2. Canine mesenteric artery and vein convey no difference in the content of major contractile proteins

    Directory of Open Access Journals (Sweden)

    Mutafova-Yambolieva Violeta N

    2002-11-01

    Full Text Available Abstract Background Mesenteric arteries and veins are composed of tonic smooth muscles and serve distinct functions in the peripheral circulation. However, the basis for the functional disparity of the resistive and capacitative parts of the mesenteric circulation is poorly understood. We studied potential differences in the expression levels of six contractile proteins in secondary and tertiary branches of the inferior mesenteric artery and vein along with differences in the vessel wall morphology. Results Bright field and electron microscopy showed that both vessel walls had the same major structural elements. The arterial walls, however, had greater number, and more tightly assembled, smooth muscle cell layers compared to vein walls. The content of actin, myosin heavy chain, myosin light chain, and calponin was similar in the two blood vessels. The artery expressed higher amount of the actin-binding protein caldesmon than the vein (41.86 ± 2.33 and 30.13 ± 3.37 μg/mg respectively, n = 12. Although the total tropomyosin content was almost identical in both blood vessels, the alpha isoform dominated in the artery, while the beta isoform prevailed in the vein. Conclusions Canine mesenteric artery and vein differ in vessel wall morphology but do not convey differences in the expression levels of actin, myosin light chain, myosin heavy chain and calponin. The two vascular networks express distinct amounts of caldesmon and tropomyosin, which might contribute to the fine tuning of the contractile machinery in a manner consistent with the physiological functions of the two vascular networks.

  3. Direct, differential effects of tamoxifen, 4-hydroxytamoxifen, and raloxifene on cardiac myocyte contractility and calcium handling.

    Directory of Open Access Journals (Sweden)

    Michelle L Asp

    Full Text Available Tamoxifen (Tam, a selective estrogen receptor modulator, is in wide clinical use for the treatment and prevention of breast cancer. High Tam doses have been used for treatment of gliomas and cancers with multiple drug resistance, but long QT Syndrome is a side effect. Tam is also used experimentally in mice for inducible gene knockout in numerous tissues, including heart; however, the potential direct effects of Tam on cardiac myocyte mechanical function are not known. The goal of this study was to determine the direct, acute effects of Tam, its active metabolite 4-hydroxytamoxifen (4OHT, and related drug raloxifene (Ral on isolated rat cardiac myocyte mechanical function and calcium handling. Tam decreased contraction amplitude, slowed relaxation, and decreased Ca²⁺ transient amplitude. Effects were primarily observed at 5 and 10 μM Tam, which is relevant for high dose Tam treatment in cancer patients as well as Tam-mediated gene excision in mice. Myocytes treated with 4OHT responded similarly to Tam-treated cells with regard to both contractility and calcium handling, suggesting an estrogen-receptor independent mechanism is responsible for the effects. In contrast, Ral increased contraction and Ca²⁺ transient amplitudes. At 10 μM, all drugs had a time-dependent effect to abolish cellular contraction. In conclusion, Tam, 4OHT, and Ral adversely and differentially alter cardiac myocyte contractility and Ca²⁺ handling. These findings have important implications for understanding the Tam-induced cardiomyopathy in gene excision studies and may be important for understanding effects on cardiac performance in patients undergoing high-dose Tam therapy.

  4. Spatio-temporal changes of lymphatic contractility and drainage patterns following lymphadenectomy in mice.

    Directory of Open Access Journals (Sweden)

    Sunkuk Kwon

    Full Text Available OBJECTIVE: To investigate the redirection of lymphatic drainage post-lymphadenectomy using non-invasive near-infrared fluorescence (NIRF imaging, and to subsequently assess impact on metastasis. BACKGROUND: Cancer-acquired lymphedema arises from dysfunctional fluid transport after lymphadenectomy performed for staging and to disrupt drainage pathways for regional control of disease. However, little is known about the normal regenerative processes of the lymphatics in response to lymphadenectomy and how these responses can be accelerated, delayed, or can impact metastasis. METHODS: Changes in lymphatic "pumping" function and drainage patterns were non-invasively and longitudinally imaged using NIRF lymphatic imaging after popliteal lymphadenectomy in mice. In a cohort of mice, B16F10 melanoma was inoculated on the dorsal aspect of the paw 27 days after lymphadenectomy to assess how drainage patterns affect metastasis. RESULTS: NIRF imaging demonstrates that, although lymphatic function and drainage patterns change significantly in early response to popliteal lymph node (PLN removal in mice, these changes are transient and regress dramatically due to a high regenerative capacity of the lymphatics and co-opting of collateral lymphatic pathways around the site of obstruction. Metastases followed the pattern of collateral pathways and could be detected proximal to the site of lymphadenectomy. CONCLUSIONS: Both lymphatic vessel regeneration and co-opting of contralateral vessels occur following lymphadenectomy, with contractile function restored within 13 days, providing a basis for preclinical and clinical investigations to hasten lymphatic repair and restore contractile lymphatic function after surgery to prevent cancer-acquired lymphedema. Patterns of cancer metastasis after lymphadenectomy were altered, consistent with patterns of re-directed lymphatic drainage.

  5. Functional effects of 17alpha-hydroxyprogesterone caproate (17P on human myometrial contractility in vitro

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    Morrison John J

    2004-12-01

    Full Text Available Abstract Background 17alpha-hydroxyprogesterone caproate (17P administration reportedly improves outcome for women with a previous spontaneous preterm delivery. This study, using in vitro strips of human uterine smooth muscle, aimed to investigate the direct non-genomic effects of 17P on spontaneous and induced contractions in tissues obtained during pregnancy, and in the non-pregnant state. Methods Biopsies of human myometrium were obtained at elective cesarean section, and from hysterectomy specimens, and dissected strips suspended for isometric recordings. The effects of 17P (1 nmol/L -10 micro mol/L on spontaneous and agonist-induced (oxytocin 0.5 nmol/L for pregnant, phenylephrine 10 μmol/L for non-pregnant contractions were measured. Integrals of contractile activity, including the mean maximal inhibition values (MMI observed at the maximal concentration, were compared with those from simultaneously run control strips. Results There was no significant direct effect exerted by 17P on pregnant or non-pregnant human myometrial contractility. The MMI ± SEM for spontaneous contractions in pregnant myometrium was 4.9% ± 7.2 (n = 6; P = 0.309 and for oxytocin-induced contractions was 2.2% ± 1.3 (n = 6; P = 0.128. For non-pregnant myometrium, the MMI ± SEM for spontaneous contractions was 8.8% ± 11.0 (n = 6; P = 0.121 and for phenylephrine induced contractions was -7.9% ± 6.5 (n = 6; P = 0.966. Conclusions The putative benefits of 17P for preterm labor prevention are not achieved, even partially, by a direct utero-relaxant effect. These findings outline the possibility that genomic effects of 17P, achieved over long periods of administration, are required for its reported therapeutic benefits.

  6. Effect of aerobic exercise on the contractile function of gastrocnemius myosin heavy chain

    Institute of Scientific and Technical Information of China (English)

    Wen-jun Ren

    2009-01-01

    Objective To study the effect of 4- 6 weeks' treadmill training of male SD rats on the contractile function of their gnstroenemius myosin heavy chain (MHC). Methods Forty male SD rats were randomly divided into control group and training group. The treadmill training of the training group rats was incessantly performed for 4- 6 weeks at an intensity of about 75% VO2max (18. 5- 24 m/min, gradient of 0°, each training session lasting 50 minutes, twice a day). The content of gastrocnemlas MHC mRNA was tested by reverse transcription polymernse chain reaction (RT-PCR), and the changes of muscle fibre and its cross-section area (CSA) were measured using immunohistochemistry. Electric stimulation tests were used to determine the maximal tension of isometric contraction of the post-training gastrocnemius. Results ① After continuous treadmill training for 4 - 6 weeks, we found that the content of the total MHC, MHC Ⅰ , MHC Ⅱ x, MHC Ⅱ a mRNAs was 105%, 105%, 109% and 108% of that in the resting control group, respectively, and the MHC Ⅱ b mRNA content did not change significantly. The percentage of MHC Ⅰ mRNA in the total MHC mRNA increased while that of MHC Ⅱ mRNA decreased after aerobic training. ② The slow type of fibre type Ⅰ was the main part of the MHC after training and the CSA of the muscle fibres increased simultaneously. ③ The maximal tension of isometric contraction by pulse stimulation of square wave in the training group increased significantly compared with that in the control group (P<0. 01). Conclusion The findings indicate that aerobic exercise may promote an increase in the contractile function of MHC.

  7. Right atrial contractile dynamics are impaired in patients with postcapillary pulmonary hypertension

    Science.gov (United States)

    Bening, Constanze; Leyh, Rainer

    2016-01-01

    Left ventricular (LV) dysfunction in conjunction with postcapillary pulmonary hypertension (PH) is frequently associated with right ventricular (RV) dysfunction, determining the patient prognosis. Compensatory mechanisms for RV dysfunction have not been previously evaluated in detail. Since calcium dependent right atrial (RA) dynamics are a surrogate for RA contractile properties, the present study examined the calcium dependency of RA tissue obtained from patients with or without postcapillary PH. In total, 15 patients with PH (PH group; mean age, 70.7±7.2 years) and 10 patients without postcapillary PH (non-PH group; mean age, 55.7±11.8 years) who were scheduled to undergo elective left heart valve surgery were included in the current study. Calcium concentration (pCa; shown as the negative log10) against force curves were generated, while LV and RV function was evaluated by echocardiography. Echocardiography data revealed a significantly reduced LV function in the PH group, while the RV function was preserved in the two groups, precluding overt RV dysfunction. In the PH group, significantly reduced force values were detected at high pCa values when compared with the non-PH group force, indicating impaired RA function. Furthermore, reduced calcium sensitivity was observed (which was determined as the pCa at half maximal activation) in the PH group, and the presence of a compensatory mechanism for reduced force capacity was hypothesized. In conclusion, the preliminary results of the current study showed impaired RA contractile properties in postcapillary hypertension with preserved RV function. The diminished RA compensatory mechanisms may lead to accelerated RV dysfunction in the clinical course of postcapillary PH.

  8. Análisis de la capacidad de aceleración en mujeres atletas de modalidades de velocidad

    Directory of Open Access Journals (Sweden)

    Gerard Carmona Dalmases

    2012-03-01

    Full Text Available Objetivos. Caracterizar la capacidad de aceleración con relación al rendimiento (tiempo en sprint de 30 m, la velocidad máxima y las manifestaciones de fuerza (capacidad de salto y potencia (en ½ squat musculares en 4 mujeres atletas de especialidades de velocidad de nivel nacional. Métodos. Se realizaron dos días de pruebas (pista y laboratorio. Se valoró la capacidad de aceleración (velocidades instantánea a 1, 2 y 3 s –vi1, vi2 i vi3–, aceleración inicial (ainicial, tiempo de sprint en 30 m (rendimiento, velocidad máxima (vmàx, capacidad de salto (SJ, CMJ, LJ bw y RJ 5s y potencia media máxima (Pm màx desarrollada en ½ squat. Resultados. Se hallaron correlaciones significativas entre el rendimiento (t30m y la vmáx (r = –0,980; P < 0,01, la capacidad de aceleración (velocidades instantáneas y el t30m (r = –0,954; P < 0,05 y la vmáx (r = 0,992 y 0,979; P < 0,01 y 0,05, la manifestación elástica de la fuerza (CMJ y el t30m (r = –0,983; P < 0,05 y la fuerza dinámica máxima relativa y la ainicial (r = 0,980; P < 0,01. Conclusiones. El principal hallazgo del presente estudio fue que la vmáx era el mayor determinante del t30m. También la capacidad de aceleración, sobretodo la vi2, y el CMJ tuvieron una gran ascendencia sobre el t30m y la vmáx.

  9. Determinantes de la capacidad de innovación en PYMES regionales Determinants of innovativeness in regional SMES

    Directory of Open Access Journals (Sweden)

    Carlos Maria Fernández-Jardón

    2013-03-01

    Full Text Available DOI: 10.5902/198346597689La capacidad de innovación es una competencia distintiva que permite innovar y, en consecuencia, potencia la competitividad, especialmente en las pequeñas y medianas empresas (PYME. Dicha capacidad se ve condicionada por factores internos y externos. Este artículo analiza como combina la empresa dichos factores para mejorar la capacidad de innovación. Por un lado, la empresa genera una competencia distintiva denominada gestión de los recursos humanos y tecnológicos a partir del capital intelectual interno. Por otro lado, organiza otra competencia distintiva denominada gestión de los recursos y relaciones territoriales a partir de los recursos tangibles asociados al territorio y del capital intelectual fruto de las relaciones con el entorno. Mediante técnicas de Mínimos cuadrados parciales aplicados  en una muestra de pequeñas y medianas empresas en una región del noroeste de España se demuestra que ambos factores influyen en la capacidad de innovación. Este estudio contribuye a delimitar cuales son los factores que potencian la capacidad de innovación y como la empresa asocia esos factores, indicando cual es el proceso de construcción de competencias básicas para mejorar su capacidad de innovación. Como consecuencia, sugiere elementos de reflexión para elaborar políticas de apoyo a la innovación empresarial.The innovativeness is a core competence to innovate and therefore enhancing competitiveness, especially in small and medium enterprises (SMEs. This capability is constrained by internal and external factors. This article discusses how the company combines these factors to improve innovativeness. First, the company generates a core competence called human resource management and technology from internal intellectual capital. Furthermore, the company organizes another core competence called resource management and territorial relations from tangible resources associated with intellectual capital

  10. Occupational stress and work capacity of nurses of a hospital group Estrés laboral y capacidad para el trabajo de enfermeros de un grupo hospitalario Estresse laboral e capacidade para o trabalho de enfermeiros de um grupo hospitalar

    Directory of Open Access Journals (Sweden)

    Christian Negeliskii

    2011-06-01

    Full Text Available The study aimed to evaluate the relationship between occupational stress and the work capacity index of 368 nurses (82.1% of the population of a Hospital Group. A questionnaire with socio-occupational questions was used as well as two scales: the Job Stress Scale and the Work Capacity Index. The group of nurses was predominantly female (93.2%, worked in care activities (63.9%, had undertaken at least one Postgraduate course (76% and were satisfied with the unit where they worked (70.5%. Occupational stress was present in 23.6% of the nurses, of these 15.2% presented High Strain work and 8.4% Passive Work. Social Support exercised a significant positive influence on all groups - exposed or not to occupational stress. Regarding the Work Capacity, this was Moderate (28-36 points for 51.4% of the nurses and Good (37-43 points for 47.4%. There was no correlation between stress and work capacity.Se trata de un estudio con objetivo de evaluar la relación entre el estrés laboral y el índice de capacidad para el trabajo de 368 enfermeros (82,1% de la población de un Grupo Hospitalario. Se utilizó un cuestionario con cuestiones socio ocupacionales y dos escalas: la Job Stress Scale y el Índice de Capacidad para el Trabajo. El grupo de enfermeros es predominantemente femenino (93,2%, trabaja en actividades asistenciales (63,9%, posee por lo menos un curso de posgraduación (76% y está satisfecho con la unidad donde trabaja (70,5%. El estrés laboral está presente en 23,6% de los enfermeros, de estos 15,2% presentan Alta Exigencia en el trabajo y 8,4% Trabajo Pasivo. El Apoyo Social ejerce influencia positiva significativa sobre todos los grupos expuestos y no expuestos al estrés laboral. En cuanto a la Capacidad para el Trabajo, está es Moderada (28-36 puntos para 51,4% de los enfermeros y Buena (37-43 puntos para 47,4%. No se encontró correlación entre el estrés y la capacidad para el trabajo.Este estudo teve como objetivo avaliar a rela

  11. Protective effects of anisodamine on cigarette smoke extract-induced airway smooth muscle cell proliferation and tracheal contractility

    International Nuclear Information System (INIS)

    Anisodamine, an antagonist of muscarinic acetylcholine receptors (mAChRs), has been used therapeutically to improve smooth muscle function, including microvascular, intestinal and airway spasms. Our previous studies have revealed that airway hyper-reactivity could be prevented by anisodamine. However, whether anisodamine prevents smoking-induced airway smooth muscle (ASM) cell proliferation remained unclear. In this study, a primary culture of rat ASM cells was used to evaluate an ASM phenotype through the ability of the cells to proliferate and express contractile proteins in response to cigarette smoke extract (CSE) and intervention of anisodamine. Our results showed that CSE resulted in an increase in cyclin D1 expression concomitant with the G0/G1-to-S phase transition, and high expression of M2 and M3. Functional studies showed that tracheal hyper-contractility accompanied contractile marker α-SMA high-expression. These changes, which occur only after CSE stimulation, were prevented and reversed by anisodamine, and CSE-induced cyclin D1 expression was significantly inhibited by anisodamine and the specific inhibitor U0126, BAY11-7082 and LY294002. Thus, we concluded that the protective and reversal effects and mechanism of anisodamine on CSE-induced events might involve, at least partially, the ERK, Akt and NF-κB signaling pathways associated with cyclin D1 via mAChRs. Our study validated that anisodamine intervention on ASM cells may contribute to anti-remodeling properties other than bronchodilation. -- Highlights: ► CSE induces tracheal cell proliferation, hyper-contractility and α-SMA expression. ► Anisodamine reverses CSE-induced tracheal hyper-contractility and cell proliferation. ► ERK, PI3K, and NF-κB pathways and cyclin D1 contribute to the reversal effect.

  12. Cardiac-specific catalase overexpression rescues anthrax lethal toxin-induced cardiac contractile dysfunction: role of oxidative stress and autophagy

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    Kandadi Machender R

    2012-11-01

    Full Text Available Abstract Background Lethal and edema toxins secreted by Bacillus anthracis during anthrax infection were found to incite serious cardiovascular complications. However, the underlying mechanisms in anthrax lethal toxin-induced cardiac anomalies remain unknown. This study was designed to evaluate the impact of antioxidant enzyme catalase in anthrax lethal toxin-induced cardiomyocyte contractile dysfunction. Methods Wild type (WT and cardiac-specific catalase overexpression mice were challenged with lethal toxin (2 μg/g, intraperotineally (i.p.. Cardiomyocyte contractile and intracellular Ca2+ properties were assessed 18 h later using an IonOptix edge-detection system. Proteasome function was assessed using chymotrypsin-like and caspase-like activities. GFP-LC3 puncta and Western blot analysis were used to evaluate autophagy and protein ubiquitination. Results Lethal toxin exposure suppressed cardiomyocyte contractile function (suppressed peak shortening, maximal velocity of shortening/re-lengthening, prolonged duration of shortening/re-lengthening, and impaired intracellular Ca2+ handling, the effects of which were alleviated by catalase. In addition, lethal toxin triggered autophagy, mitochondrial and ubiquitin-proteasome defects, the effects of which were mitigated by catalase. Pretreatment of cardiomyocytes from catalase mice with the autophagy inducer rapamycin significantly attenuated or ablated catalase-offered protection against lethal toxin-induced cardiomyocyte dysfunction. On the other hand, the autophagy inhibitor 3-MA ablated or significantly attenuated lethal toxin-induced cardiomyocyte contractile anomalies. Conclusions Our results suggest that catalase is protective against anthrax lethal toxin-induced cardiomyocyte contractile and intracellular Ca2+ anomalies, possibly through regulation of autophagy and mitochondrial function.

  13. Protective effects of anisodamine on cigarette smoke extract-induced airway smooth muscle cell proliferation and tracheal contractility

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Guang-Ni; Yang, Kai; Xu, Zu-Peng; Zhu, Liang; Hou, Li-Na; Qi, Hong; Chen, Hong-Zhuan, E-mail: hongzhuan_chen@hotmail.com; Cui, Yong-Yao, E-mail: yongyaocui@yahoo.com.cn

    2012-07-01

    Anisodamine, an antagonist of muscarinic acetylcholine receptors (mAChRs), has been used therapeutically to improve smooth muscle function, including microvascular, intestinal and airway spasms. Our previous studies have revealed that airway hyper-reactivity could be prevented by anisodamine. However, whether anisodamine prevents smoking-induced airway smooth muscle (ASM) cell proliferation remained unclear. In this study, a primary culture of rat ASM cells was used to evaluate an ASM phenotype through the ability of the cells to proliferate and express contractile proteins in response to cigarette smoke extract (CSE) and intervention of anisodamine. Our results showed that CSE resulted in an increase in cyclin D1 expression concomitant with the G0/G1-to-S phase transition, and high expression of M2 and M3. Functional studies showed that tracheal hyper-contractility accompanied contractile marker α-SMA high-expression. These changes, which occur only after CSE stimulation, were prevented and reversed by anisodamine, and CSE-induced cyclin D1 expression was significantly inhibited by anisodamine and the specific inhibitor U0126, BAY11-7082 and LY294002. Thus, we concluded that the protective and reversal effects and mechanism of anisodamine on CSE-induced events might involve, at least partially, the ERK, Akt and NF-κB signaling pathways associated with cyclin D1 via mAChRs. Our study validated that anisodamine intervention on ASM cells may contribute to anti-remodeling properties other than bronchodilation. -- Highlights: ► CSE induces tracheal cell proliferation, hyper-contractility and α-SMA expression. ► Anisodamine reverses CSE-induced tracheal hyper-contractility and cell proliferation. ► ERK, PI3K, and NF-κB pathways and cyclin D1 contribute to the reversal effect.

  14. Effects of Crocetin Esters and Crocetin from Crocus sativus L. on Aortic Contractility in Rat Genetic Hypertension

    Directory of Open Access Journals (Sweden)

    Silvia Llorens

    2015-09-01

    Full Text Available Background: Endothelial dysfunction, characterized by an enhancement in vasoconstriction, is clearly associated with hypertension. Saffron (Crocus sativus L. bioactive compounds have been recognized to have hypotensive properties. Recently, we have reported that crocetin exhibits potent vasodilator effects on isolated aortic rings from hypertensive rats. In this work, we have aimed to analyze the anticontractile ability of crocetin or crocetin esters pool (crocins isolated from saffron. Thus, we have studied the effects of saffron carotenoids on endothelium-dependent and -independent regulation of smooth muscle contractility in genetic hypertension. Methods: We have measured the isometric responses of aortic segments with or without endothelium obtained from spontaneously hypertensive rats. The effects of carotenoids were studied by assessing the endothelial modulation of phenylephrine-induced contractions (10−9–10−5 M in the presence or absence of crocetin or crocins. The role of nitric oxide and prostanoids was analyzed by performing the experiments with L-NAME (NG-nitro-l-arginine methyl ester or indomethacin (both 10−5 M, respectively. Results: Crocetin, and to a minor extent crocins, diminished the maximum contractility of phenylephrine in intact rings, while crocins, but not crocetin, increased this contractility in de-endothelizated vessels. In the intact vessels, the effect of crocetin on contractility was unaffected by indomethacin but was abolished by L-NAME. However, crocetin but not crocins, lowered the already increased contractility caused by L-NAME. Conclusions: Saffron compounds, but especially crocetin have endothelium-dependent prorelaxing actions. Crocins have procontractile actions that take place via smooth muscle cell mechanisms. These results suggest that crocetin and crocins activate different mechanisms involved in the vasoconstriction pathway in hypertension.

  15. CAPACIDAD DE SALTO EN NIÑAS PREPÚBERES QUE PRACTICAN GIMNASIA RÍTMICA

    Directory of Open Access Journals (Sweden)

    R. Arteaga

    2010-10-01

    Full Text Available

    Estudiamos el rendimiento en el salto vertical y como podría verse afectado por lacomposición corporal en 13 niñas que practicaban gimnasia rítmica (GR; 10.4 ± 0.9 años y13 niñas control (CO; 9.9 ± 0.7 años. La composición corporal fue determinada medianteantropometría y DXA. Se realizaron saltos con y sin contramovimiento (CMJ y SJ sobre unaplataforma de fuerza analizándose entre otras variables la altura de vuelo (AV, velocidad dedespegue (VD, velocidad vertical máxima del centro de masas (Vimax, la potencia media(Pm, el impulso mecánico positivo (Ipos, tiempo de fuerza máxima (Tfmax y potenciainstantánea máxima (Pimax. Las gimnastas consiguieron una AV, VD, Ipos y Vimax mayoren ambos saltos y una Pm, Tfmax y Pimax mayores en el CMJ que las control (p<0.05. Enconclusión, prácticar gimnasia rítmica 10 h·sem-1 se asocia a un mayor rendimiento en elsalto vertical.

    Palabras claves: gimnasia rítmica, entrenamiento de fuerza, capacidad de salto.

  16. DESARROLLO DE UNA MANO ROBÓTICA CON CAPACIDAD DE MANIPULACIÓN

    Directory of Open Access Journals (Sweden)

    Iván Pérez

    2008-09-01

    Full Text Available El área que ocupa la presente investigación está relacionada con la robótica, con los tipos de robots autónomos y androide; son autónomos o inteligentes debido a que procesa la información; convirtiéndose en más evolucionados porque ellos responde de acuerdo al ambiente en que se presente sin la intervención del hombre o con una pequeña intervención y androide por que se tratara de imitar una parte del cuerpo humano. Para llevar a cabo un robot con dicha característica es necesario trabajar en el sistema mecánico del mismo teniendo en cuenta la cantidad de articulaciones que permite la movilidad y el control de la misma, se trabaja con la electrónica, que admite controlar el robot y procesar la información para que su respuesta sea la correcta; este estudio, se planteó como objetivo general desarrollo de una mano robótica con capacidad de manipulación que permita imitar la mano humana tocando piezas musicales en un piano digital, para el abordaje metodológico se aplicó la metodología y herramientas para el diseño de sistemas digitales, diseños de circuitos y sistemas electrónicos.

  17. Hysteroscopic sterilization with occlusion of sheep uterine tube using n-butyl-2-cyanoacrylate adhesive Esterilização histeroscópica com oclusão da tuba uterina de ovelha com adesivo de n-butil-2-cianoacrilato

    Directory of Open Access Journals (Sweden)

    Sergio Bigolin

    2007-10-01

    Full Text Available PURPOSE: To evaluate the fertility and analyze the macroscopic, microscopic and morphometric aspects of sheep uterine tube sterilization with a hysteroscopically insert of n-butyl-2-cyanoacrylate adhesive. METHODS: 12 adult sheep, with one previous pregnancy, were distributed as follows: group L (n=3 subjected to laparotomy and Pomeroy uterine tube ligation, group S (n=3 subjected to hysteroscopic application of saline solution in tube isthmus and group AD(n=6, that was subjected to hysteroscopic application of 0.5 ml of n-2-butil-cyanoacrylate in tube isthmus. They were mated with fertile males for ninety days. The non pregnant sheep, at the 90th day, were subjected to laparotomy with uterus and tubes uterine resection. The fragments of uterine tubes were fixated in 10% formalin and processes for histology evaluated, and slices dyes for H.E. Data were evaluated by Wilcoxon and Mann-Whitney and Fisher's exact test. RESULTS: All sheep from groups L and AD did not get pregnant (0% in contrast with sheep from group S (100%; the adhesive remained integral in the uterine tube lumen. The percentual of adherences (66.6% and fibrosis responses (100% was significantly higher in the group L than group AD (0% (pOBJETIVO: Avaliar a fertilidade e aspectos macroscópicos, microscópicos e morfométricos da esterilização histeroscópica de tubas uterinas de ovelhas com o adesivo de n-butil-2-cianoacrilato. MÉTODOS: 12 ovelhas adultas, com uma prenhez anterior, foram distribuídas como segue: o grupo L (n=3 submetidas à laparotomia e laqueadura tipo Pomeroy, grupo S (n=3 submetidas à aplicação histeroscópica de solução salina no istmo tubário e grupo AD (n=6, com aplicação histeroscópica de 0,5 ml de cianoacrilato. As ovelhas foram acasaladas com machos de comprovada fertilidade por noventa dias. As ovelhas não prenhes aos 90 dias, foram submetidas à laparotomia com ressecção do útero e tubas uterinas, que foram fixadas em formalina 10%s e os

  18. Qual o melhor período para a realização do doppler das artérias uterinas na predição de complicações da gestação? Which is the best period to perform uterine artery Doppler in the prediction of pregnancy complications?

    Directory of Open Access Journals (Sweden)

    Fabrício da Silva Costa

    2006-04-01

    Full Text Available OBJETIVO: Avaliar, prospectivamente, qual o melhor período para a realização do Doppler das artérias uterinas na predição de complicações da gestação. MATERIAIS E MÉTODOS: Foi conduzido estudo prospectivo em 45 pacientes primigestas, sem história de doenças crônicas. O Doppler das artérias uterinas foi realizado entre 18-20, 24-26, 28-30 e 34-36 semanas, com determinação do índice de resistência, índice de pulsatilidade, relação A/B e a presença ou ausência de incisura na onda de velocidade de fluxo, assim como o resultado da gestação. RESULTADOS: Os índices Doppler apresentaram decréscimo com o decorrer da gestação, mais pronunciado nas gestações normais quando estas foram comparadas com aquelas que tiveram complicações, de maneira mais acentuada no exame realizado entre 24-26 semanas. A melhor relação entre sensibilidade e especificidade para a detecção de complicações durante a gestação foi obtida no exame realizado entre 24-26 semanas. CONCLUSÃO: O melhor período para a realização do Doppler das artérias uterinas na predição de complicações da gestação é o intervalo entre 24-26 semanas.OBJECTIVE: To estimate which is the best period to realize uterine artery Doppler for the prediction of pregnancy complications. MATERIALS AND METHODS: A prospective study was conducted with 45 women without a history of chronic diseases and in their first pregnancy. The uterine artery Doppler was performed between the pregnancy weeks 18-20, 24-26, 28-30 and 34-36, with determination of the resistance index, the pulsatility index, the A/B ratio and presence or absence of notch on the flow velocity waveform, as well as the result of the pregnancy. RESULTS: The Doppler index showed decrease in the course of the pregnancy, more pronounced in the normal pregnancies in comparison with the complicated pregnancies. This was most noticed in the examinations performed between the weeks 24-26. The best sensitivity

  19. Capacidades Estatais no Programa Bolsa Família: O Desafio de Consolidação do Sistema Único de Assistência Social

    OpenAIRE

    Diogo R. Coutinho

    2013-01-01

    Por meio de uma abordagem centrada na análise de arranjos político-institucionais criados para implementar políticas de desenvolvimento no contexto democrático pós-1988, este trabalho utiliza as categorias de capacidades técnico-administrativas (associadas à dimensão de efetividade) e capacidades políticas (associadas à dimensão de legitimidade) para descrever o Programa Bolsa Família (PBF) em sua interação com o campo da assistência social. Discute como se relacionam tais capacidades no plan...

  20. CREENCIA DE CAPACIDAD y SATISFACCIÓN PERSONAL COMO ELEMENTOS PARA EVALUAR LA PERCEPCIÓN DE LA CALIDAD DE VIDA DE LOS HABITANTES EN LOS MOCHIS, SINALOA

    OpenAIRE

    Marysol Morales Valenzuela; Beatriz Yasuko Arita Watanabe

    2012-01-01

    En el presente estudio se aborda la percepción de la calidad de vida, considerando la creencia de capacidad para enfrentar la vida así como medir los niveles de satisfacción por la vida de habitantes de la ciudad de Los Mochis, Sinaloa. Se utilizó la escala de creencia de capacidad que evalúa la creencia que el individuo tiene para enfrentar la vida en cuanto a su salud, educación, estatus social, capacidad para el trabajo y nivel cultural y The Australian Unity Well-being, de la cual solo ut...

  1. Las nuevas tecnologías de la información Especial referencia a las empresas con capacidad de aprendizaje (Learning Organizations)

    OpenAIRE

    Jeremias Dutschke, Georg Michaez

    2006-01-01

    El objetivo de nuestro estudio es validar la hipótesis fundamental de que las organizaciones con más capacidad de aprendizaje utilizan el Internet, Intranet y Correo Electrónico, más tiempo y para más funciones, que las organizaciones con menos capacidad de aprendizaje. Basado en la Teoría de los Recursos y Capacidades (Pfeffer y Salancik, 1978) y en el Mo delo de Aprendizaje de Senge (1990), utilizando la análisis de correlaciones y el test de hipótesis, se intentará validar la hipótesis fun...

  2. EFICACIA EN EL DESARROLLO DE CAPACIDADES TIC EN ESTUDIANTES DE EDUCACIÓN SECUNDARIA DE LIMA, PERÚ

    Directory of Open Access Journals (Sweden)

    Raúl Choque Larrauri

    2009-01-01

    Full Text Available Se evaluó la eficacia de la implementación de las Aulas de Innovación Pedagógica en el desarrollo de capacidades en Tecnologías de la Información y la Comunicación (TIC, en los estudiantes de educación secundaria de Lima. La investigación fue cuasi experimental, con posprueba, con grupo de comparación. La población de estudio estuvo constituido por 1 141 estudiantes del 4to. y 5to. grado de educación secundaria de una red educativa de Lima. Se confirmó la hipótesis de que los estudiantes desarrollaron las capacidades TIC de adquisición de la información, trabajo en equipo y el desarrollo de estrategias de aprendizaje con TIC.

  3. Representações de alunos sobre o domínio de capacidades matemáticas

    OpenAIRE

    Nogueira, Sofia; Tenreiro-Vieira, Celina; Cabrita, Isabel

    2010-01-01

    Reconhecendo a importância de promover as capacidades de resolução de problemas e de comunicação (em) matemática dos alunos, potenciando conexões entre as áreas de matemática e ciências físicas e naturais e articulando contextos de educação formal e não formal, desenvolveu-se um estudo com a finalidade de avaliar o impacto de situações de exploração matemática de módulos interactivos de ciências no desenvolvimento de capacidades matemáticas ligadas à resolução de problemas e à comunicação, po...

  4. Capacidades de detección de las herramientas de análisis de vulnerabilidades en aplicaciones web

    OpenAIRE

    Román Muñoz, Fernando; Sabido Cortés, Iván Israel; García Villalba, Luis Javier

    2014-01-01

    Debido al continuo incremento del número de vulnerabilidades en las aplicaciones Web, se han elaborado diversas clasificaciones para mantener organizadas estas vulnerabilidades, y también se han desarrollado herramientas para detectarlas. Hasta este momento, según nuestra información, no se ha realizado ningún estudio sobre las capacidades de estas herramientas en la detección de las vulnerabilidades presentes en las clasificaciones de vulnerabilidades. En este trabajo mapeamos y agrupamos la...

  5. Alumnos con altas capacidades musicales en Educación Secundaria Obligatoria: una iniciativa de acción

    OpenAIRE

    Campo-Quiralte, Cristina

    2013-01-01

    La presente investigación tiene como objetivo conocer las principales características del alumnado con Necesidades Educativas Especiales, principalmente en aquellos que poseen Altas Capacidades Musicales. Para ello, se definen y elaboran las herramientas indispensables confeccionando medidas de atención personalizadas mediante el desarrollo de materiales y recursos específicos, destacando el papel y actuación de la familia que resulta indispensable para llevar a cabo una educación satisfac...

  6. Reproducibilidad en la lectura de un set de placas de citología cérvico-uterinas en cuatro centros especializados de Medellín, Antioquia / Reproducibility of reading a set of cervical cytology smears in four specialized centers in Medellin, Antioquia

    Directory of Open Access Journals (Sweden)

    Edwin Guevara

    2014-05-01

    Full Text Available Objetivo: evaluar el grado de reproducibilidad en la lectura de placas de citología cérvico-uterina entre cuatro centros de lectura especializados de la ciudad de Medellín, Colombia. Metodología: 181 placas de citología cérvico-uterina provenientes de un estudio de prevalencia de anormalidades citológicas en Pueblorrico, Antioquia, se sometieron a lectura en cuatro centros especializados de Medellín. Se le pidió a cada centro que realizara una lectura rutinaria manteniendo el estudio en ciego por medio de recodificación de las placas, evitando que se filtrara información entre centros. Se calculó la concordancia general y el índice kappa de Fleiss. Resultados: de las 181 placas, sólo en 55 placas los 4 centros concordaron en el resultado, obteniéndose un porcentaje de concordancia global del 30% y un índice kappa global de 0,31. Según la escala de Fleiss, se observó una baja reproducibilidad en la lectura de las placas citológicas entre los cuatro centros involucrados en el estudio. La concordancia por pares de centros presentó índices de kappa entre 0,3 a 0,7. Discusión: existe una alta variabilidad en la interpretación de los resultados citológicos entre los centros estudiados. Es necesario implementar procesos de entrenamiento y unificación de criterios de lecturas de la citología cervical en nuestro medio Objective: to assess the degree of reproducibility in the reading of cervical cytology smears among four specialized reading centers at Medellin, Colombia. Methodology: 181 cervical cytology smears from a study on the prevalence of cytological abnormalities in Pueblorrico, a town located in the southwestern region of the state of Antioquia in Colombia, were re-read by four specialized centers in Medellin. Each center was asked to conduct a blind routine reading of the smears to avoid disclosure of information between centers. The reproducibility was measured by percentage agreement and kappa. Results: of 181 smears

  7. Expressão da capacidade de empatia em bebês de 8 e 18 semanas

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    Kornilia Hatzinikolaou

    2006-04-01

    Full Text Available Este estudo investigou o padrão temporal e expressivo da capacidade de empatia em bebês de 8 e 18 semanas no contexto de interações face-face entre 90 duplas de mãe-bebê. As interações foram analisadas em tempo real e através de micro-análise. Alguns comportamentos infantis apresentaram maior probabilidade de fazerem parte do padrão expressivo da empatia do que outros. Ainda, o padrão expressivo se manifestou diferente em instâncias de empatia com emoções negativas e instâncias de empatia com emoções positivas comunicadas pela mãe. Em relação ao padrão temporal, observou-se que a manifestação da capacidade de empatia ocorreu dentro de um intervalo de tempo igual de ½ segundo antes e 1 segundo depois da mudança emocional da mãe. Os resultados indicam a necessidade de reavaliação das proposições tradicionais sobre as capacidades comunicativas precoces de bebês, e sugerem uma coerência entre os achados e as proposições teóricas contemporâneas na área da Psicologia Infantil.

  8. Persistencia y capacidad predictiva de márgenes y rotaciones. un análisis empírico

    Directory of Open Access Journals (Sweden)

    Juan Monterrey

    2011-01-01

    Full Text Available En el presente trabajo abordamos el estudio empírico de la persistencia y capacidad predictiva de los componentes margen y rotación de la rentabilidad. A partir de una amplia muestra de compañías españolas, hemos documentado cómo la persistencia de la rotación es superior a la del margen, y cómo los componentes anormales de margen y rotación son menos persistentes que sus componentes sectoriales. Con relación a la capacidad predictiva, nuestros hallazgos confirman que el poder de predicción del margen es más elevado que el de la rotación, aunque su persistencia sea menor. Al separar los componentes sectorial y anormal de margen y rotación hemos comprobado cómo los componentes anormales muestran una capacidad predictiva superior a los componentes sectoriales. Los análisis complementarios muestran el distinto poder predictivo según el signo positivo o negativo de márgenes y rotaciones anormales, y según el signo del resultado, así como la robustez de nuestros hallazgos.

  9. Capacidade antioxidante de hortaliças usualmente consumidas Antioxidant capacity of vegetables commonly consumed

    Directory of Open Access Journals (Sweden)

    Enayde de Almeida Melo

    2006-09-01

    Full Text Available Como objetivo de avaliar a capacidade antioxidante de 15 hortaliças comercializadas na Cidade do Recife, extratos metanólicos foram testados quanto a atividade antioxidante em sistema modelo beta-caroteno/ácido linoléico e a habilidade de seqüestrar o radical estável 1,1-difenil-2-picrilhidrazil (DPPH. Todas as hortaliças investigadas exibiram propriedade antioxidante, entretanto a ação foi diferenciada entre os vegetais. Os extratos metanólicos da couve folha, tomate, batata, couve-flor, repolho verde, espinafre e alface crespa, com percentual de inibição superior a 70%, foram os mais eficazes em seqüestrar o radical livre. Os extratos metanólicos da alface lisa, cebola branca e vagem apresentaram ação moderada (60-70% de inibição, enquanto que a cebola roxa, chuchu, pepino e cenoura exibiram a mais fraca capacidade de seqüestrar o radical DPPH. No sistema modelo beta-caroteno/ácido linoléico, os extratos metanólicos do espinafre e couve-folha exibiram a mais elevada atividade antioxidante (superior a 70%. Ação antioxidante moderada (60-70% foi exibida pelos extratos da alface lisa, cebola branca e couve-flor, enquanto que os do chuchu, cenoura, pepino, tomate e vagem, com atividade inferior a 60%, foram considerados com fraca ação antioxidante. As hortaliças testadas podem ser vistas como fontes dietéticas de antioxidantes que podem trazer benefícios à saúde, portanto o seu consumo deve ser estimulado.This study was carried out to determine the antioxidant capacity of 15 vegetables commonly consumed in Recife - PE, Brazil. Methanol extracts were screened for their antioxidant activity using two tests: DPPH free radical scavenging and beta-carotene/linoléico acid assay. All vegetables showed antioxidant properties however the action was differentiated among the kinds of vegetables. The methanol extracts of collard greens, tomatoes, potatoes, cauliflowers, green cabbage, spinach and lettuce "crespa" had the

  10. Vasodilatory effects of nifedipine, methoxyverapamil, and sodium nitroprusside on contractile responses of the ewe uterine artery at term pregnancy.

    Science.gov (United States)

    Isla, M; Dyer, D C

    1990-10-01

    The differential inhibitory effect of the vasodilators on contractile responses to norepinephrine, serotonin, and potassium on isolated uterine artery ring segments from pregnant ewes within 2 weeks of term was quantified and correlated with the source of Ca++ for the vasoconstrictors producing the smooth muscle contraction. The contraction evoked by the vasoconstrictors was dependent on extracellular Ca++ and in agonist-induced contractions also on an intracellular pool of Ca++. Nifedipine effectively inhibited K(+)-induced (90 mmol/L) contractions (antagonist concentration to reduce the maximum contractile effect to the agonist to 50%, 1.95 +/- 0.9 x 10(-8) mol/L), whereas it was relatively ineffective in blocking norepinephrine-induced (10(-5) mol/L) or serotonin-induced (10(-5) mol/L) vasoconstriction (antagonist concentration to reduce the maximum contractile effect to the agonist to 50%, 1.38 +/- 0.4 x 10(-4) mol/L and 2.04 +/- 0.4 x 10(-5) mol/L, respectively). Methoxyverapamil (D-600) strongly inhibited serotonin-induced contractions (antagonist concentration to reduce the maximum contractile effect to the agonist to 50%, 3.3 +/- 0.3 x 10(-7) mol/L). The phasic rather than the tonic components of the serotonin- and norepinephrine-induced contractions were more effectively inhibited by D-600 (p less than 0.05). Sodium nitroprusside preferentially blocked (p less than 0.05) the sustained tonic components of norepinephrine- and serotonin-induced vasoconstrictions (antagonist concentration to reduce the maximum contractile effect to the agonist to 50%, 7.1 +/- 0.4 x 10(-7) mol/L and 8.2 +/- 0.6 x 10(-7) mol/L, respectively). On the basis of these findings it is concluded that D-600 and sodium nitroprusside are more effective than nifedipine in blocking contractile responses due to receptor stimulation, and therefore might be more effective in the treatment of hypertensive emergencies in which these amines might be implicated. PMID:2220945

  11. Confiabilidade teste-reteste do Indice de Capacidade para o Trabalho (ICT em trabalhadores de enfermagem

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    Sergio Henrique Almeida da Silva Junior

    2013-03-01

    Full Text Available Este artigo avalia a confiabilidade teste-reteste do Índice de Capacidade para o Trabalho (ICT em trabalhadores de enfermagem. Foi aplicado questionário autopreenchível duas vezes a um grupo de 80 trabalhadores de enfermagem (enfermeiros, técnicos e auxiliares de enfermagem de um hospital público no Município do Rio de Janeiro, com intervalo entre sete e quinze dias. A confiabilidade foi estimada pela estatística kappa ponderada quadrática, pelo coeficiente de correlação intraclasse (CCI e pelo gráfico de Bland e Altman. Dos participantes, 81% eram mulheres e com idade variando de 22 a 67 anos (média = 39,1; DP = 10,8anos e 36,3% tinham ensino superior completo. O escore global do ICT apresentou CCI = 0,79 (IC95% 0,67 a 0,86 e kappa = 0,69 (IC95% 0,50 a 0,80 para o ICT categórico (classificado em baixo, moderado, bom e ótimo. O kappa dos itens do ICT variou de 0,39 a 0,82 e o gráfico de Bland e Altman não mostrou um padrão sistemático. A concordância entre as medidas de teste-reteste indica grau aceitável de confiabilidade, sugerindo adequação do processo de aferição entre trabalhadores de enfermagem.

  12. Brote del virus ISA: crisis ambiental y capacidad de la institucionalidad ambiental para manejar el conflicto

    Directory of Open Access Journals (Sweden)

    Beatriz Bustos

    2012-09-01

    Full Text Available La industria salmonera chilena vivió recientemente una crisis de magnitud a partir de la aparición del virus ISA. El artículo examina esta situación, concentrándose en el papel que desempeña la ciencia en las soluciones políticas propuestas, como caso para discutir la capacidad de los mecanismos de gobernanza ambiental neoliberal para solucionar crisis ambientales y económicas. La pregunta que guía el trabajo es: ¿cuál fue el rol de la ciencia en la política ambiental para la industria? El argumento promovido es que la crisis representa un momento de reestructuración de la relación sociedad-naturaleza, en el cual se redefinieron mecanismos de propiedad, control y acceso a recursos naturales, pero en el que, pese al discurso neoliberal de confianza en los expertos y la ciencia como elementos de toma de decisiones, la información científica tuvo escasa relevancia.The Chilean salmon industry recently experienced a crisis due to an outbreak of the ISA virus. The article examines the crisis focusing on the role of science in the policy solutions proposed, to discuss the real capacity of neoliberal environmental governance mechanisms to solve environmental and economic crisis. The question guiding this research is, which was the role of science in environmental policy createdfor the salmon industry? The argument is that the crisis represents a reestructuring of the relationship between society and nature through the redefinition of property rights, control and access to natural resources, but in which, in spite of a neoliberal discourse of trust in experts and science as elements of decision making, scientific information had little relevance.

  13. Capacidade antioxidante e qualidade pós-colheita de abacate 'Fuerte'

    Directory of Open Access Journals (Sweden)

    Rogério Lopes Vieites

    2012-06-01

    Full Text Available A capacidade antioxidante e a qualidade pós-colheita do abacate 'Fuerte' foram avaliadas. Os frutos foram mantidos sob temperatura ambiente (24 ± 1 ºC e sob refrigeração em 10 ± 1 ºC e 90±5% UR e avaliados durante 15 dias. Determinaram-se a perda de massa e a taxa respiratória. As características físico-químicas avaliadas foram acidez total titulável (AT, sólidos solúveis (SS, ratio, pH, firmeza, atividade da enzima polifenoloxidase (PPO, cor (L, a* e b*, fenólicos totais e atividade antioxidante (AA por DPPH·. Nas duas condições de armazenamento, o pico respiratório ocorreu no 9º dia.Os frutos mantidos sob temperatura refrigerada apresentaram menor perda de massa e maior firmeza em relação àqueles em temperatura ambiente. O comportamento respiratório refletiu na redução dos valores de AT e no conteúdo de SS que diminuíram a partir do pico de respiração. Os valores de pH aumentaram como consequência da diminuição da AT. Os valores de luminosidade mantiveram-se elevados, observando-se diminuição do componente de cor verde na polpa com o armazenamento. A atividade da PPO diminuiu com o armazenamento. Os valores de AA variaram de 17,6 a 68,7%. O conteúdo de fenólicos totais diminuiu após o pico respiratório, e valores crescentes de AA foram observados mesmo após o 9º dia.

  14. Efecto de las estrategias competitivas y los recursos y capacidades orientados al mercado sobre el crecimiento de las organizaciones

    Directory of Open Access Journals (Sweden)

    Carmen Berenice Ynzunza Cortés

    2013-01-01

    Full Text Available Este estudio analiza las relaciones entre las fuerzas de mercado, las estrategias competitivas, los recursos y capacidades orientados al mercado, así como el desempeño organizacional. La investigación se realizó en Pyme del sector industrial del estado de Querétaro, con un muestreo de conveniencia no probabilístico; asimismo, se diseñó un modelo estructural para probar las relaciones entre las variables, las cuales se analizaron con el software AMOS. El tamaño de muestra alcanzado fue de 116 y la recopilación de información se hizo mediante entrevistas. Las fuerzas de mercado, la estrategia competitiva y la orientación a mercado fueron medidas con las escalas de Kohly y Javorsky (1990, Segev (1987 y Narver y Slater (1990. Los ítems para el desempeño organizacional y los recursos de tecnología e innovación fueron diseñados. Los hallazgos prueban que las fuerzas de mercado tienen un impacto grande en la estrategia competitiva, el cual no es significativo sobre los recursos y capacidades, y es negativo respecto al desempeño, medido por el crecimiento organizacional. El efecto de la estrategia competitiva ha sido significativo sobre los recursos y capacidades de orientación a mercado, tecnología e innovación y no significativo respecto al desempeño. Estos recursos y capacidades influyen de manera significativa en el desempeño organizacional. Los resultados de este estudio enfatizan la implementación de estrategias prospectoras y analizadoras, que crean recursos y capacidades que den valor, con beneficios económicos para las empresas, así como los de orientación a mercado, tecnología e innovación. Estos hallazgos son consistentes con los encontrados en otras investigaciones realizadas en otros países.

  15. Dietary fat influences the expression of contractile and metabolic genes in rat skeletal muscle.

    Directory of Open Access Journals (Sweden)

    Wataru Mizunoya

    Full Text Available Dietary fat plays a major role in obesity, lipid metabolism, and cardiovascular diseases. To determine whether the intake of different types of dietary fats affect the muscle fiber types that govern the metabolic and contractile properties of the skeletal muscle, we fed male Wistar rats with a 15% fat diet derived from different fat sources. Diets composed of soybean oil (n-6 polyunsaturated fatty acids (PUFA-rich, fish oil (n-3 PUFA-rich, or lard (low in PUFAs were administered to the rats for 4 weeks. Myosin heavy chain (MyHC isoforms were used as biomarkers to delineate the skeletal muscle fiber types. Compared with soybean oil intake, fish oil intake showed significantly lower levels of the fast-type MyHC2B and higher levels of the intermediate-type MyHC2X composition in the extensor digitorum longus (EDL muscle, which is a fast-type dominant muscle. Concomitantly, MyHC2X mRNA levels in fish oil-fed rats were significantly higher than those observed in the soybean oil-fed rats. The MyHC isoform composition in the lard-fed rats was an intermediate between that of the fish oil and soybean oil-fed rats. Mitochondrial uncoupling protein 3, pyruvate dehydrogenase kinase 4, and porin mRNA showed significantly upregulated levels in the EDL of fish oil-fed rats compared to those observed in soybean oil-fed and lard-fed rats, implying an activation of oxidative metabolism. In contrast, no changes in the composition of MyHC isoforms was observed in the soleus muscle, which is a slow-type dominant muscle. Fatty acid composition in the serum and the muscle was significantly influenced by the type of dietary fat consumed. In conclusion, dietary fat affects the expression of genes related to the contractile and metabolic properties in the fast-type dominant skeletal muscle, where the activation of oxidative metabolism is more pronounced after fish oil intake than that after soybean oil intake.

  16. Itraconazole decreases left ventricular contractility in isolated rabbit heart: Mechanism of action

    Energy Technology Data Exchange (ETDEWEB)

    Qu, Yusheng, E-mail: yqu@amgen.com [Toxicology Science, Amgen, Inc, One Amgen Center Drive, Thousand Oaks, CA 91320 (United States); Fang, Mei; Gao, BaoXi; Amouzadeh, Hamid R. [Toxicology Science, Amgen, Inc, One Amgen Center Drive, Thousand Oaks, CA 91320 (United States); Li, Nianyu; Narayanan, Padma [Discovery Toxicology, Amgen, Inc, One Amgen Center Drive, Thousand Oaks, CA 91320 (United States); Acton, Paul; Lawrence, Jeff; Vargas, Hugo M. [Toxicology Science, Amgen, Inc, One Amgen Center Drive, Thousand Oaks, CA 91320 (United States)

    2013-04-15

    Itraconazole (ITZ) is an approved antifungal agent that carries a “black box warning” in its label regarding a risk of negative cardiac inotropy based on clinical findings. Since the mechanism of the negative inotropic effect is unknown, we performed a variety of preclinical and mechanistic studies to explore the pharmacological profile of ITZ and understand the negative inotropic mechanism. ITZ was evaluated in: (1) an isolated rabbit heart (IRH) preparation using Langendorff retrograde perfusion; (2) ion channel studies; (3) a rat heart mitochondrial function profiling screen; (4) a mitochondrial membrane potential (MMP) assay; (5) in vitro pharmacology profiling assays (148 receptors, ion channels, transporters, and enzymes); and (6) a kinase selectivity panel (451 kinases). In the IRH, ITZ decreased cardiac contractility (> 30%) at 0.3 μM, with increasing effect at higher concentrations, which indicated a direct negative inotropic effect upon the heart. It also decreased heart rate and coronary flow (≥ 1 μM) and prolonged PR/QRS intervals (3 μM). In mechanistic studies, ITZ inhibited the cardiac NaV channel (IC{sub 50}: 4.2 μM) and was devoid of any functional inhibitory effect at the remaining pharmacological targets. Lastly, ITZ did not affect MMP, nor interfere with mitochondrial enzymes or processes involved with fuel substrate utilization or energy formation. Overall, the cardiovascular and mechanistic data suggest that ITZ-induced negative inotropy is a direct effect on the heart, in addition, the potential involvement of mitochondria function and L-type Ca{sup 2+} channels are eliminated. The exact mechanism underlying the negative inotropy is uncertain, and requires further study. - Highlights: ► Effect of itraconazole (ITZ) was assessed in the isolated rabbit heart (IRH) assay. ► ITZ decreased ventricular contractility in IRH, indicating a direct effect. ► IC{sub 50} of ITZ on L-type I{sub Ca} was greater than 30 μM, on I{sub Na} was 4

  17. Sensibilidad a la temperatura de los fenoles totales y de la capacidad antioxidante y antirradicalaria de los hongos comestibles Clitocybe geotropa, Hygrophorus gliocyclus y Clitocybe alexandrii

    OpenAIRE

    Tufano, Aniello

    2013-01-01

    Medida de los fenoles totales, la capacidad antioxidante y antirradicalaria de las setas comestibles Clitocybe geotropa, Hygrophorus gliocyclus, Clitocybe alexandrii, mediante los métodos Folin–Ciocalteau, CUPRAC y DPPH y estudio de la degradación a diferentes temperaturas y tiempos de los fenoles totales y pérdida de la capacidad antioxidante y antirradicalaria de, Clitocybe geotropa, Hygrophorus gliocyclus, Clitocybe alexandrii, mediante los métodos Folin–Ciocalteau, CUPRA...

  18. Gestión intergubernamental y capacidad estratégica en el desarrollo local y regional fronterizo

    Directory of Open Access Journals (Sweden)

    José María Ramos García

    2002-01-01

    Full Text Available El artículo tiene como objetivo principal examinar el papel de los gobiernos locales en el desarrollo re g i onal de la frontera norte de México, con base en los enfoques de la gestión interg u b e rnamental de políticas p ú blicas y la capacidad estrat é g i c a . La ap o rtación teórica de este artículo consiste en destacar y especificar el papel de la gestión en las políticas de desarrollo fronterizo, con base en la capacidad de los recursos humanos, administrativa y, en ge n e r a l , g u b e rn a m e n t a l . A s i m i s m o, se fundamenta la gestión interg u b e rn am e ntal en mat e ria de asuntos fro n t e ri z o s , en especial en el ámbito local y a su ve z , la gestión de pro blemas transf ro n t e ri z o s. La pregunta central del artículo es: ¿los gobiernos fronterizos cuentan con las capacidades para promover el desarrollo regional en un contexto fronterizo y transf ro n t e rizo? La premisa que se plantea concibe que una mayor y eficaz cooperación entre los tres ámbitos de g o b i e rno (local, e s t atal y fe d e r a l , en mat e ria de desarrollo re g i o n a l , y sustentada en una capacidad estrat ég i c a , c o a dy u va a una mejor gestión e implantación de las políticas. Los temas generales del artículo son: 1.Antecedentes de las teorías de crecimiento y de política regional, donde se destaca la importancia de la gestión y políticas públicas en las principales teorías del crecimiento y de la política regional; 2. Gestión pública, capacidad y gestión intergubernamental;se examinan algunos antecedentes generales de la gestión pública y se define el concepto de capacidad gubernamental y su impacto en el desarrollo regional, así como la importancia de la gestión intergubernamental, y 3. Problemas fronterizos y nuevos enfoques en la gestión del desarrollo regional; se analizan algunos problemas fro n t e ri z o s , con la finalidad de destacar ciert a s limitaciones en la capacidad gubern a

  19. Endothelin-1 and endothelin-2 initiate and maintain contractile responses by different mechanisms in rat mesenteric and cerebral arteries

    DEFF Research Database (Denmark)

    Compeer, M. G.; Janssen, G. M. J.; De Mey, J. G. R.

    2013-01-01

    by stimulation of soluble GC, AC or K+-channels and by an inhibitor of receptor-operated ion channels. However, each of these reduced maintenance of ET-1 effects and relaxed ET-1-induced contractions in MRA. A calcium channel antagonist did not alter sensitivity, maximum and maintenance of ET-1......Background and PurposeEndothelin (ET)-1 and ET-2 cause potent long-lasting vasoconstrictions by tight binding to smooth muscle ETA receptors. We tested the hypotheses that different mechanisms mediate initiation and maintenance of arterial contractile responses to ET-1 and ET-2 and that this...... effects, but relaxed ET-1-induced contractions in MRA. A PLC inhibitor prevented contractile responses to ET-1 and ET-2 in MRA and BA, and relaxed ET-1- and ET-2-induced responses in MRA and ET-1 effects in BA. A Rho-kinase inhibitor did not modify sensitivity, maximum and maintenance of responses to both...

  20. Usage of echocardiography with physical loads for diagnosis of myocardial contractile reserve of the left ventricle in athletes

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    Nekhanevich O.B.

    2014-09-01

    Full Text Available The work purpose was studying of myocardial contractile reserve of the left ventricle and cardiohemodynamics infringements character under the influence of physical loads in athletes with functional insufficiency of mitral valve according to stress-echocardiography. We examined 72 athletes the aged 9 to 40 years with functional mitral valve insufficiency and normal systolic function of the heart at rest by echo ECG data. Possibility of stress echocardiography with physical loads usage to diagnose decrease of myocardial contractile reserve of the heart left ventricle was proved. It was found that increase in hemodynamic load during physical exercise leads to the disruption of adaptation and manifestation of systolic dysfunction in athletes with I and II degrees of mitral valve regurgitation. This should be considered when constructing training-competitive loads among athletes in terms of prevention of acute physical overloading.

  1. Alterações histoquímicas das glicosaminoglicanas na cérvice uterina no final da prenhez da rata albina após ministração local de hialuronidase Histochemical changes of the glycosaminoglycans in the uterine cervix of pregnant rats after local injection of hyaluronidase

    Directory of Open Access Journals (Sweden)

    Viviane Almeida de Alcântara Lopes

    2008-07-01

    Full Text Available OBJETIVO: estudar as alterações histoquímicas relacionadas às glicosaminoglicanas da cérvice uterina da rata albina, após ministração local de hialuronidase no final da prenhez. MÉTODOS: dez ratas com teste de prenhez positivo foram distribuídas aleatoriamente em dois grupos, numericamente iguais. O Grupo Controle (Gc foi constituído pelas ratas que receberam 1 mL de água destilada, dose única, no 18º dia da prenhez, sob anestesia, ministrado na cérvice uterina. O Grupo Experimental (Gex constou de ratas que receberam, sob as mesmas condições do Gc, 0,02 mL de hialuronidase, diluído em 0,98 mL de água destilada (total de 1 mL. No 20º dia de prenhez, as ratas foram novamente anestesiadas e submetidas à dissecção, preparando-se a cérvice uterina para estudo histoquímico com coloração de alcian blue e seus bloqueios (pH=0,5, pH=2,5, metilação e saponificação. RESULTADOS: verificou-se na lâmina própria no Gc, reação fortemente positiva (+3 e, no Gex, reação negativa, na coloração de alcian blue no pH=0,5. Em pH=2,5 a coloração também se apresentou fortemente positiva (+4 no Gc e fracamente positiva (+1 no Gex. Após metilação, tanto o Gc quanto o Gex mostraram reação negativa após coloração de alcian blue no pH=2,5. Com a reação de metilação seguida de saponificação e na digestão enzimática em lâmina, a coloração da lâmina própria se mostrou negativa em ambos os grupos. CONCLUSÕES: há uma nítida predominância de glicosaminoglicanas sulfatadas no Gc em relação ao Gex e uma tênue quantidade de glicosaminoglicanas carboxiladas identificadas no Gex. As modificações evidenciadas na matriz extracelular sugerem que a hialuronidase injetada localmente na cérvix uterina promoveu alterações bioquímicas compatíveis com maturação cervical.PURPOSE: to study the histochemical changes related to the uterine cervix glycosaminoglycan of the albino female rat, after local ministration of

  2. Avaliação da cavidade uterina com hístero-sonografia em pacientes com falha de implantação após fertilização in vitro Evaluation of the uterine cavity by sonohysterography in patients with implantation failure after in vitro fertilization

    Directory of Open Access Journals (Sweden)

    Teiichi Ninomiya

    2003-08-01

    Full Text Available OBJETIVO: analisar a acurácia da hístero-sonografia (HSoG na avaliação da cavidade uterina nas pacientes que tiveram falha de implantação na primeira tentativa de fertilização in vitro. MÉTODOS: foram analisadas prospectivamente, em estudo duplo-cego, pacientes previamente submetidas à transferência de pelo menos um embrião e que apresentaram falha de implantação. As pacientes foram submetidas a HSoG e em seguida a histeroscopia diagnóstica, realizadas por equipes diferentes, não havendo troca de informações sobre os resultados das mesmas. Os resultados foram agrupados e interpretados somente após o término da pesquisa. A HSoG foi realizada após cateterização do colo uterino por sonda uretral nº 8 e injeção de soro fisiológico. A interface anecóica proporcionada pela solução salina permite determinar as anormalidades, tais como pólipos uterinos ou miomas submucosos. A histeroscopia diagnóstica foi realizada com equipamento Karl Storz, óptica rígida de 4 mm a 30°, e distensão da cavidade uterina com soro fisiológico. Não utilizou-se anestesia local nem houve a necessidade de realizar dilatação cervical. RESULTADOS: foram estudadas 28 pacientes das 33 inicialmente selecionadas para o presente estudo. A HSoG diagnosticou anormalidades em 8 pacientes, sendo que em cinco pacientes foram encontrados pólipos endometriais (62,5%, pólipos endocervicais em duas pacientes (25,0% e mioma submucoso em uma (12,5%. A histeroscopia (padrão-ouro diagnosticou alterações em 7 pacientes, sendo que em duas pacientes (28,6% foram encontrados pólipos endometriais, em outras duas pacientes (28,6% pólipos cervicais e em uma paciente, mioma submucoso (14,2%. A HSoG, quando comparada com a histeroscopia diagnóstica, apresentou sensibilidade de 71,4%, especificidade de 85,7%, valor preditivo positivo de 62,5% e valor preditivo negativo de 90%. CONCLUSÕES: por apresentar valor preditivo positivo baixo, todas as vezes que a

  3. SK channel-selective opening by SKA-31 induces hyperpolarization and decreases contractility in human urinary bladder smooth muscle

    OpenAIRE

    Soder, Rupal P; Parajuli, Shankar P.; Hristov, Kiril L.; Eric S. Rovner; Petkov, Georgi V.

    2012-01-01

    Overactive bladder (OAB) is often associated with increased involuntary detrusor smooth muscle (DSM) contractions during the bladder-filling phase. To develop novel therapies for OAB, it is critical to better understand the mechanisms that control DSM excitability and contractility. Recent studies showed that small-conductance Ca2+-activated K+ (SK) channels, SK3 channels, in particular, regulate human DSM function. However, the concept that SK channel-selective pharmacological activation can...

  4. Role of Alpha-actinin-3 in Contractile Properties of Human Single Muscle Fibers: A Case Series Study in Paraplegics

    OpenAIRE

    Broos, Siacia; Malisoux, Laurent; Theisen, Daniel; Francaux, Marc; Deldicque, Louise; Thomis, Martine

    2012-01-01

    A common nonsense polymorphism in the ACTN3 gene results in the absence of α-actinin-3 in XX individuals. The wild type allele has been associated with power athlete status and an increased force output in numeral studies, though the mechanisms by which these effects occur are unclear. Recent findings in the Actn3(-/-) (KO) mouse suggest a shift towards 'slow' metabolic and contractile characteristics of fast muscle fibers lacking α-actinin-3. Skinned single fibers from the quadriceps muscle ...

  5. Efficacy and Safety of Intravesical OnabotulinumtoxinA Injection in Patients with Detrusor Hyperactivity and Impaired Contractility

    OpenAIRE

    Chung-Cheng Wang; Cheng-Ling Lee; Hann-Chorng Kuo

    2016-01-01

    We investigated the efficacy and safety of intravesical onabotulinumtoxinA injection in patients with detrusor hyperactivity and impaired contractility (DHIC). Twenty-one patients with urodynamically proven DHIC and 21 age-matched patients with overactive bladder (OAB) with urodynamic detrusor overactivity were treated with intravesical injections of 100 U of onabotulinumtoxinA. The overactive bladder symptom score, urgency severity score, patient perception of bladder condition, global respo...

  6. Dynamic dyssynchrony and impaired contractile reserve of the left ventricle in beta-thalassaemia major: an exercise echocardiographic study.

    Directory of Open Access Journals (Sweden)

    Yiu-fai Cheung

    Full Text Available BACKGROUND: Performance of the left ventricle during exercise stress in thalassaemia patients is uncertain. We aimed to explore the phenomenon of dynamic dyssynchrony and assess contractile reserve in patients with beta-thalassaemia major and determine their relationships with myocardial iron load. METHODS AND RESULTS: Thirty-two thalassaemia patients (16 males, aged 26.8 ± 6.9 years, without heart failure and 17 healthy controls were studied. Their left ventricular (LV volumes, ejection fraction, systolic dyssynchrony index (SDI, and myocardial acceleration during isovolumic LV contraction (IVA were determined at rest and during submaximal bicycle exercise testing using 3-dimensional and tissue Doppler echocardiography. Myocardial iron load as assessed by T2* cardiac magnetic resonance in patients were further related to indices of LV dyssynchrony and contractile reserve. At rest, patients had significantly greater LV SDI (p4.6%, control+2SD increased from baseline 25% to 84% in patients. Δ SDI(exercise-baseline correlated with exercise-baseline differences in LV ejection fraction (p<0.001 and stroke volume (p = 0.006. Compared with controls, patients had significantly less exercise-induced increase in LV ejection fraction, cardiac index, and IVA (interaction, all p<0.05 and had impaired contractile reserve as reflected by the gentler IVA-heart rate slope (p = 0.018. Cardiac T2* in patients correlated with baseline LV SDI (r = -0.44, p = 0.011 and IVA-heart rate slope (r = 0.36, p = 0.044. CONCLUSIONS: Resting LV dyssynchrony is associated with myocardial iron load. Exercise stress further unveils LV dynamic dyssynchrony and impaired contractile reserve in patients with beta-thalassaemia major.

  7. Acute contractile recovery extent during biventricular pacing is not associated with follow-up in patients undergoing resynchronization

    OpenAIRE

    Federica DeVecchi; Emanuela Facchini; Anna Degiovanni; Chiara Sartori; Chiara Cavallino; Matteo Santagostino; Virginia Di Ruocco; Andrea Magnani; Eraldo Occhetta; Paolo Nicola Marino

    2016-01-01

    Background: It has been reported that contractility, as assessed using dobutamine infusion, is independently associated with reverse remodeling after CRT. Controversy, however, exists about the capacity of this approach to predict a long-term clinical response. This study's purpose was to assess whether long-term CRT clinical effects can be predicted according to acute inotropic response induced by biventricular stimulation (CRT on), as compared with AAI–VVI right stimulation pacing mode (CRT...

  8. Molecular mechanical differences between isoforms of contractile actin in the presence of isoforms of smooth muscle tropomyosin.

    OpenAIRE

    Lennart Hilbert; Genevieve Bates; Roman, Horia N.; Jenna L Blumenthal; Zitouni, Nedjma B.; Apolinary Sobieszek; Mackey, Michael C.; Anne-Marie Lauzon

    2013-01-01

    The proteins involved in smooth muscle's molecular contractile mechanism - the anti-parallel motion of actin and myosin filaments driven by myosin heads interacting with actin - are found as different isoforms. While their expression levels are altered in disease states, their relevance to the mechanical interaction of myosin with actin is not sufficiently understood. Here, we analyzed in vitro actin filament propulsion by smooth muscle myosin for [Formula: see text]-actin ([Formula: see text...

  9. Coupled expression of troponin T and troponin I isoforms in single skeletal muscle fibers correlates with contractility

    OpenAIRE

    Brotto, Marco A.; Biesiadecki, Brandon J.; Brotto, Leticia S.; Nosek, Thomas M.; Jin, J.-P.

    2005-01-01

    Brotto, Marco A., Brandon J. Biesiadecki, Leticia S. Brotto, Thomas M. Nosek, and J.-P. Jin. Striated muscle contraction is powered by actin-activated myosin ATPase. This process is regulated by Ca2+ via the troponin complex. Slow and fast twitch fibers of vertebrate skeletal muscle express type I and type II myosin, respectively, and these myosin isoenzymes confer different ATPase activities, contractile velocities and force. Skeletal muscle troponin has also diverged into fast and slow isof...

  10. Anthrax lethal toxin suppresses murine cardiomyocyte contractile function and intracellular Ca2+ handling via a NADPH oxidase-dependent mechanism.

    Directory of Open Access Journals (Sweden)

    Machender R Kandadi

    Full Text Available OBJECTIVES: Anthrax infection is associated with devastating cardiovascular sequelae, suggesting unfavorable cardiovascular effects of toxins originated from Bacillus anthracis namely lethal and edema toxins. This study was designed to examine the direct effect of lethal toxins on cardiomyocyte contractile and intracellular Ca(2+ properties. METHODS: Murine cardiomyocyte contractile function and intracellular Ca(2+ handling were evaluated including peak shortening (PS, maximal velocity of shortening/ relengthening (± dL/dt, time-to-PS (TPS, time-to-90% relengthening (TR(90, intracellular Ca(2+ rise measured as fura-2 fluorescent intensity (ΔFFI, and intracellular Ca(2+ decay rate. Stress signaling and Ca(2+ regulatory proteins were assessed using Western blot analysis. RESULTS: In vitro exposure to a lethal toxin (0.05-50 nM elicited a concentration-dependent depression on cardiomyocyte contractile and intracellular Ca(2+ properties (PS, ± dL/dt, ΔFFI, along with prolonged duration of contraction and intracellular Ca(2+ decay, the effects of which were nullified by the NADPH oxidase inhibitor apocynin. The lethal toxin significantly enhanced superoxide production and cell death, which were reversed by apocynin. In vivo lethal toxin exposure exerted similar time-dependent cardiomyocyte mechanical and intracellular Ca(2+ responses. Stress signaling cascades including MEK1/2, p38, ERK and JNK were unaffected by in vitro lethal toxins whereas they were significantly altered by in vivo lethal toxins. Ca(2+ regulatory proteins SERCA2a and phospholamban were also differentially regulated by in vitro and in vivo lethal toxins. Autophagy was drastically triggered although ER stress was minimally affected following lethal toxin exposure. CONCLUSIONS: Our findings indicate that lethal toxins directly compromised murine cardiomyocyte contractile function and intracellular Ca(2+ through a NADPH oxidase-dependent mechanism.

  11. THE EFFECT OF HYPOXIA ON ELECTRICAL AND CONTRACTILE PROPERTIES OF SMOOTH MUSCLES OF THE GUINEA PIG URETER

    Directory of Open Access Journals (Sweden)

    I. V. Kovalev

    2016-01-01

    Full Text Available Aim. The effect of hypoxia on the electrical and contractile activities of smooth muscles cells (SMCs of the guinea pig ureter was studied by the method of the double sucrose bridge.Materials and methods. This method allows registering simultaneously parameters of the action potential (AP and the contraction of SMCs, caused by an electrical stimulus.Results. It was found that lowering the oxygen content in the perfusion solution for 10 min resulted to an increase of electrical and contractile activity of ureteral SMCs. Addition of tetraethylammonium chloride (TEA, 5 mM – nonselective blocker of potassium membrane conductance – in hypoxic conditions causing an additional increase in the amplitude of the AP, duration of the AP plateau and the contractile responses of smooth muscles. Thus, the hypoxia decreased the potassium membrane conductance of ureteral SMCs. Inhibition of the effect of the α1 -adrenergic receptors agonist phenylephrine (PE, 10 mM on the electrical and contractile properties of SMCs in hypoxic condition indicate the involvement of the protein kinase C-dependent signaling system in effects of hypoxia. Pretreatment of ureteral smooth muscles with bumetanide (100 mM – selective inhibitor of Na+,K+,2Cl- - cotransporter (NKCC – caused a decrease of the activating effect of hypoxia on the SMCs of guinea pig ureter.Conclusion.Thus, the impact of hypoxia on the regulation of electrical activity and contractions of smooth muscles of guinea pig ureter may be due to changes in ion permeability of membranes SMCs and operation of ion-transporting systems. 

  12. H1-histamine receptors may mediate the contractile response of guinea-pig ileum to 'histamine-free' splenic extracts.

    OpenAIRE

    Ainz, L. F.; Casis, E.; de Gandarias, J. M.; Gil-Rodrigo, C. E.; Goiriena de Gandarias, J. J.

    1983-01-01

    A water-soluble splenic factor, which produces a contractile response of the guinea-pig ileum, that is resistant to cholinoceptor and adrenoceptor antagonists is described. The ileal contractions elicited by the splenic extract showed some significant differences from those elicited by 5-hydroxytryptamine. The responses to splenic extract were not affected by the D-tryptamine-receptor antagonist, methysergide. The effect of the splenic extract on the guinea-pig ileum was similar to that of hi...

  13. Time-Lapse Imaging as a Tool to Investigate Contractility of the Epididymal Duct – Effects of Cgmp Signaling

    OpenAIRE

    Andrea Mietens; Sabine Tasch; Angelika Stammler; Lutz Konrad; Caroline Feuerstacke; Ralf Middendorff

    2014-01-01

    The well orchestrated function of epididymal smooth muscle cells ensures transit of spermatozoa through the epididymal duct during which spermatozoa acquire motility and fertilizing capacity. Relaxation of smooth muscle cells is mediated by cGMP signaling and components of this pathway are found within the male reproductive tract. Whereas contractile function of caudal parts of the rat epididymal duct can be examined in organ bath studies, caput and corpus regions are fragile and make it diff...

  14. Acute exposure to Catha edulis depresses contractility and induces myocardial infarction in spontaneously contracting, isolated rabbit’s heart

    OpenAIRE

    Al-Hashem, Fahaid H.; Dallak, Mohammad A.; Nwoye, Luke O.; Bin-Jaliah, Ismaeel M; Al-Amri, Hasan S; Rezk, Mahmoud H.; Sakr, Hussein F.; Abdullah S Shatoor; Al-Khateeb, Mahmoud

    2011-01-01

    Khat chewing is a recreational habit known to pose major socio-economic and medical problems in countries of Southern Arabia and the Horn of Africa. Among other adverse health effects, khat chewing has been associated with an increased risk of myocardial infarction (MI) in heavy consumers. This study was carried out to examine the direct effects of Catha edulis extract on contractility of spontaneously contracting, isolated rabbit heart and to investigate its mechanism of action. Isolated six...

  15. Surfactant Proteins SP-A and SP-D Modulate Uterine Contractile Events in ULTR Myometrial Cell Line.

    Directory of Open Access Journals (Sweden)

    Georgios Sotiriadis

    Full Text Available Pulmonary surfactant proteins SP-A and SP-D are pattern recognition innate immune molecules. However, there is extrapulmonary existence, especially in the amniotic fluid and at the feto-maternal interface. There is sufficient evidence to suggest that SP-A and SP-D are involved in the initiation of labour. This is of great importance given that preterm birth is associated with increased mortality and morbidity. In this study, we investigated the effects of recombinant forms of SP-A and SP-D (rhSP-A and rhSP-D, the comprising of trimeric lectin domain on contractile events in vitro, using a human myometrial cell line (ULTR as an experimental model. Treatment with rhSP-A or rhSP-D increased the cell velocity, distance travelled and displacement by ULTR cells. rhSP-A and rhSP-D also affected the contractile response of ULTRs when grown on collagen matrices showing reduced surface area. We investigated this effect further by measuring contractility-associated protein (CAP genes. Treatment with rhSP-A and rhSP-D induced expression of oxytocin receptor (OXTR and connexin 43 (CX43. In addition, rhSP-A and rhSP-D were able to induce secretion of GROα and IL-8. rhSP-D also induced the expression of IL-6 and IL-6 Ra. We provide evidence that SP-A and SP-D play a key role in modulating events prior to labour by reconditioning the human myometrium and in inducing CAP genes and pro-inflammatory cytokines thus shifting the uterus from a quiescent state to a contractile one.

  16. Time-lapse imaging as a tool to investigate contractility of the epididymal duct--effects of cGMP signaling.

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    Andrea Mietens

    Full Text Available The well orchestrated function of epididymal smooth muscle cells ensures transit of spermatozoa through the epididymal duct during which spermatozoa acquire motility and fertilizing capacity. Relaxation of smooth muscle cells is mediated by cGMP signaling and components of this pathway are found within the male reproductive tract. Whereas contractile function of caudal parts of the rat epididymal duct can be examined in organ bath studies, caput and corpus regions are fragile and make it difficult to mount them in an organ bath. We developed an ex vivo time-lapse imaging-based approach to investigate the contractile pattern in these parts of the epididymal duct. Collagen-embedding allowed immobilization without impeding contractility or diffusion of drugs towards the duct and therefore facilitated subsequent movie analyses. The contractile pattern was made visible by placing virtual sections through the acquired image stack to track wall movements over time. By this, simultaneous evaluation of contractile activity at different positions of the observed duct segment was possible. With each contraction translating into a spike, drug-induced alterations in contraction frequency could be assessed easily. Peristaltic contractions were also detectable and throughout all regions in the proximal epididymis we found regular spontaneous contractile activity that elicited movement of intraluminal contents. Stimulating cGMP production by natriuretic peptide ANP or inhibiting degradation of cGMP by the phosphodiesterase 5 inhibitor sildenafil significantly reduced contractile frequency in isolated duct segments from caput and corpus. RT-PCR analysis after laser-capture microdissection localized the corresponding molecules to the smooth muscle layer of the duct. Our time-lapse imaging approach proved to be feasible to assess contractile function in all regions of the epididymal duct under near physiological conditions and provides a tool to evaluate acute

  17. Mechanism of glucose-6-phosphate dehydrogenase-mediated regulation of coronary artery contractility.

    Science.gov (United States)

    Ata, Hirotaka; Rawat, Dhwajbhadur K; Lincoln, Thomas; Gupte, Sachin A

    2011-06-01

    We previously identified glucose-6-phosphate dehydrogenase (G6PD) as a regulator of vascular smooth muscle contraction. In this study, we tested our hypothesis that G6PD activated by KCl via a phosphatase and tensin homologue deleted on chromosome 10 (PTEN)-protein kinase C (PKC) pathway increases vascular smooth muscle contraction and that inhibition of G6PD relaxes smooth muscle by decreasing intracellular Ca(2+) ([Ca(2+)](i)) and Ca(2+) sensitivity to the myofilament. Here we show that G6PD is activated by membrane depolarization via PKC and PTEN pathway and that G6PD inhibition decreases intracellular free calcium ([Ca(2+)](i)) in vascular smooth muscle cells and thus arterial contractility. In bovine coronary artery (CA), KCl (30 mmol/l) increased PKC activity and doubled G6PD V(max) without affecting K(m). KCl-induced PKC and G6PD activation was inhibited by bisperoxo(pyridine-2-carboxyl)oxovanadate (Bpv; 10 μmol/l), a PTEN inhibitor, which also inhibited (P PET-cGMPs (100 nmol/l) diminished 6AN-evoked VASP phosphorylation (P PET-cGMPs increased 6AN-induced relaxation. These findings suggest G6PD inhibition relaxes CA by decreasing Ca(2+) influx, increasing Ca(2+) sequestration, and inhibiting Rho kinase but not by increasing Ca(2+) extrusion or activating PKG. PMID:21398595

  18. Methanol extract of Tephrosia vogelii leaves potentiates the contractile action of acetylcholine on isolated rabbit jejunum

    Institute of Scientific and Technical Information of China (English)

    Tavershima Dzenda; Joseph Olusegun Ayo; Alexander Babatunde Adelaiye; Ambrose Osemattah Adaudi

    2015-01-01

    To investigate the modulating role of methanol extract of Tephrosia vogelii leaves on acetylcholine (ACh)-induced contraction of isolated rabbit jejunum. Methods: Rabbit jejunum segment was removed and placed in an organ bath containing Tyrode’s solution, and its contractions were recorded isometrically. Results: ACh (2.0 × 10-10 g/mL) and the extract (2.0 × 10-4 g/mL) individually increased the frequency of contraction (mean ± SEM) of the isolated smooth muscle tissue by 47.6% ± 9.5%and 77.8% ± 66.5%, respectively. When ACh and the extract were combined, the frequency of contraction of the tissue was increased by 222.2% ± 25.9%, representing a 366.7% increase (P < 0.001) over the effect of ACh alone. Similarly, ACh (2.0 × 10-9 g/mL) and the extract individually increased significantly (P < 0.001) the amplitude of contraction of the tissue by 685.7% ± 61.1% and 455.2% ± 38.1%, respectively. When ACh and the extract were combined, the amplitude of contraction of the tissue rose by 1263.8% ± 69.0%, representing 84.3% increase over the effect of ACh alone. Conclusions: The findings demonstrate that methanol extract of Tephrosia vogelii leaves potentiates the contractile effect of ACh on intestinal smooth muscle, supporting the traditional claim that the plant is purgative.

  19. Pulsatile cell-autonomous contractility drives compaction in the mouse embryo.

    Science.gov (United States)

    Maître, Jean-Léon; Niwayama, Ritsuya; Turlier, Hervé; Nédélec, François; Hiiragi, Takashi

    2015-07-01

    Mammalian embryos initiate morphogenesis with compaction, which is essential for specifying the first lineages of the blastocyst. The 8-cell-stage mouse embryo compacts by enlarging its cell-cell contacts in a Cdh1-dependent manner. It was therefore proposed that Cdh1 adhesion molecules generate the forces driving compaction. Using micropipette aspiration to map all tensions in a developing embryo, we show that compaction is primarily driven by a twofold increase in tension at the cell-medium interface. We show that the principal force generator of compaction is the actomyosin cortex, which gives rise to pulsed contractions starting at the 8-cell stage. Remarkably, contractions emerge as periodic cortical waves when cells are disengaged from adhesive contacts. In line with this, tension mapping of mzCdh1(-/-) embryos suggests that Cdh1 acts by redirecting contractility away from cell-cell contacts. Our study provides a framework to understand early mammalian embryogenesis and original perspectives on evolutionary conserved pulsed contractions. PMID:26075357

  20. Mechanism of contractile action of barium ion on rat aortic smooth muscle.

    Science.gov (United States)

    Ebeigbe, A B; Aloamaka, C P

    1987-12-01

    The mechanism of Ba2+-induced contraction has been examined in helical strips of Ca2+-depleted, 60 mM K+-depolarized rat aortae. The concentration-response curves to Ca2+ or Ba2+ were significantly potentiated by exposure to 3 X 10(-8) M Bay K 8644 (a Ca2+ channel agonist) in the order Ca2+ greater than Ba2+, suggesting an action of Ba2+ ions through potential-sensitive membrane Ca2+ channels. Exposure of strips to background concentration of Ca2+ (0.05 mM) enhanced the contractile responses to Ba2+, whereas background exposure to Ba2+ (0.1 mM) attenuated Ca2+ responses. Repeated stimulation with Ba2+ resulted in tachyphylaxis, contrary to the result when Ca2+ was used. The results suggest that Ba2+ ions enter rat aortic smooth muscle cells through Ca2+ channels and mobilize a noradrenaline-insensitive intracellular Ca2+ pool. Ba2+ may also cause a desensitization of some intracellular process. PMID:3449201

  1. Shortening actin filaments cause force generation in actomyosin network to change from contractile to extensile

    Science.gov (United States)

    Kumar, Nitin; Gardel, Margaret

    Motor proteins in conjunction with filamentous proteins convert biochemical energy into mechanical energy which serves a number of cellular processes including cell motility, force generation and intracellular cargo transport. In-vitro experiments suggest that the forces generated by kinesin motors on microtubule bundles are extensile in nature whereas myosin motors on actin filaments are contractile. It is not clear how qualitatively similar systems can show completely different behaviors in terms of the nature of force generation. In order to answer this question, we carry out in vitro experiments where we form quasi 2D filamentous actomyosin networks and vary the length of actin filaments by adding capping protein. We show that when filaments are much shorter than their typical persistence length (approximately 10 microns), the forces generated are extensile and we see active nematic defect propagation, as seen in the microtubule-kinesin system. Based on this observation, we claim that the rigidity of rods plays an important role in dictating the nature of force generation in such systems. In order to understand this transition, we selectively label individual filaments and find that longer filaments show considerable bending and buckling, making them difficult to slide and extend along their length.

  2. Hadp1, a newly identified pleckstrin homology domain protein, is required for cardiac contractility in zebrafish

    Directory of Open Access Journals (Sweden)

    Joshua D. Wythe

    2011-09-01

    The vertebrate heart is one of the first organs to form, and its early function and morphogenesis are crucial for continued embryonic development. Here we analyze the effects of loss of Heart adaptor protein 1 (Hadp1, which we show is required for normal function and morphogenesis of the embryonic zebrafish heart. Hadp1 is a pleckstrin homology (PH-domain-containing protein whose expression is enriched in embryonic cardiomyocytes. Knockdown of hadp1 in zebrafish embryos reduced cardiac contractility and altered late myocyte differentiation. By using optical mapping and submaximal levels of hadp1 knockdown, we observed profound effects on Ca2+ handling and on action potential duration in the absence of morphological defects, suggesting that Hadp1 plays a major role in the regulation of intracellular Ca2+ handling in the heart. Hadp1 interacts with phosphatidylinositol 4-phosphate [PI4P; also known as PtdIns(4P] derivatives via its PH domain, and its subcellular localization is dependent upon this motif. Pharmacological blockade of the synthesis of PI4P derivatives in vivo phenocopied the loss of hadp1 in zebrafish. Collectively, these results demonstrate that hadp1 is required for normal cardiac function and morphogenesis during embryogenesis, and suggest that hadp1 modulates Ca2+ handling in the heart through its interaction with phosphatidylinositols.

  3. Accuracy of Dobutamine Stress Echocardiography in Detecting Recovery of Contractile Reserve after Revascularization of Ischemic Myocardium

    Directory of Open Access Journals (Sweden)

    Abas Ali karimi

    2007-09-01

    Full Text Available Background: This study was designed to investigate the accuracy of dobutamine stress echocardiography (DSE in detecting the post-revascularization recovery rate of contractile reserve (CR in ischemic myocardium. Methods: A total of 112 segments from seven patients with low ejection fraction (<35% and coronary artery disease were evaluated with DSE one week before and 12 weeks after coronary artery bypass graft surgery (CABG. Sensitivity, specificity, and positive and negative predictive values of DSE for detecting the recovery rate of CR were calculated based upon their standard definition and were presented with 95% confidence intervals (CI. Results: The mean baseline left ventricular ejection fraction was 31±4%, which reached 35±7% after CABG unremarkably. The recovery rates of resting function and CR were 18.2% and 50% for hypokinetic and 15.6% and 24.1 for akinetic segments respectively. Specificity, sensitivity, and positive and negative predictive values of DSE for detecting the recovery of CR were 83% (CI=69-97, 89% (CI=83-96, 94% (CI = 88-99, and 73 % (CI = 55-88, respectively. Conclusion: Despite acceptable sensitivity, specificity, and positive predictive value, DSE has a relatively lower negative predictive value for detecting the recovery of CR in ischemic myocardium and, consequently, the full extent of myocardial viability. Further sensitive techniques may, therefore, be needed to provide complementary information regarding long-term functional outcome.

  4. Human embryonic stem cell derived mesenchymal progenitors express cardiac markers but do not form contractile cardiomyocytes.

    Directory of Open Access Journals (Sweden)

    Christophe M Raynaud

    Full Text Available Mesenchymal progenitors or stromal cells have shown promise as a therapeutic strategy for a range of diseases including heart failure. In this context, we explored the growth and differentiation potential of mesenchymal progenitors (MPs derived in vitro from human embryonic stem cells (hESCs. Similar to MPs isolated from bone marrow, hESC derived MPs (hESC-MPs efficiently differentiated into archetypical mesenchymal derivatives such as chondrocytes and adipocytes. Upon treatment with 5-Azacytidine or TGF-β1, hESC-MPs modified their morphology and up-regulated expression of key cardiac transcription factors such as NKX2-5, MEF2C, HAND2 and MYOCD. Nevertheless, NKX2-5+ hESC-MP derivatives did not form contractile cardiomyocytes, raising questions concerning the suitability of these cells as a platform for cardiomyocyte replacement therapy. Gene profiling experiments revealed that, although hESC-MP derived cells expressed a suite of cardiac related genes, they lacked the complete repertoire of genes associated with bona fide cardiomyocytes. Our results suggest that whilst agents such as TGF-β1 and 5-Azacytidine can induce expression of cardiac related genes, but treated cells retain a mesenchymal like phenotype.

  5. Endothelium protectant and contractile effects of the antivaricose principle escin in rat aorta.

    Science.gov (United States)

    Carrasco, Omar F; Vidrio, Horacio

    2007-07-01

    The triterpene saponin escin is the active component of the extract of seeds of Aesculus hippocastanum used in the treatment of chronic venous insufficiency. Escin is also used experimentally to increase membrane permeability in isolated cells. Since endothelial dysfunction is postulated to be involved in venous insufficiency, the possible endothelium-protectant effect of escin was explored in rat aortic rings, a model widely used to study such effects with cardiovascular agents. Escin enhanced endothelium-dependent relaxation induced by acetylcholine when such relaxation had been reduced by exposure to the superoxide ion generator pyrogallol. This effect was attributed to enhanced nitric oxide production by endothelial nitric oxide synthase, a calcium-dependent enzyme, activated by the increased endothelial cell permeability to calcium induced by escin. Another effect of escin thought to contribute to its therapeutic activity is its ability to produce venous contraction. The compound was found to induce concentration-related contraction also in rat aortic rings. This response was partially inhibited by removal of the endothelium or by preincubation with indomethacin, and was completely abolished by incubation in a calcium-free perfusion fluid. Contraction was considered to be due mainly to the aforementioned effect on calcium permeability, with some mediation by release of endothelial vasoconstrictor prostanoids. It was concluded that, in rat aorta, escin possesses an endothelium-protectant action and a direct contractile effect. The former could contribute to its beneficial effect in the treatment of venous insufficiency, while the latter could constitute a limiting side effect. PMID:17512261

  6. The contribution of Kv7 channels to pregnant mouse and human myometrial contractility.

    Science.gov (United States)

    McCallum, Laura A; Pierce, Stephanie L; England, Sarah K; Greenwood, Iain A; Tribe, Rachel M

    2011-03-01

    Premature birth accounts for approximately 75% of neonatal mortality and morbidity in the developed world. Despite this, methods for identifying and treating women at risk of preterm labour are limited and many women still present in preterm labour requiring tocolytic therapy to suppress uterine contractility. The aim of this study was to assess the utility of Kv7 channel activators as potential uterine smooth muscle (myometrium) relaxants in tissues from pregnant mice and women. Myometrium was obtained from early and late pregnant mice and from lipopolysaccharide (LPS)-injected mice (day 15 of gestation; model of infection in pregnancy). Human myometrium was obtained at the time of Caesarean section from women at term (38-41 weeks). RT-PCR/qRT-PCR detected KCNQ and KCNE expression in mouse and human myometrium. In mice, there was a global suppression of all KCNQ isoforms, except KCNQ3, in early pregnancy (n= 6, P flupirtine (20 μM, Kv7 channel activators) caused profound myometrial relaxation (P < 0.05). In summary, Kv7 activators suppressed myometrial contraction and KCNQ gene expression was sustained throughout gestation, particularly at term. Consequently, activation of the encoded channels represents a novel mechanism for treatment of preterm labour. PMID:20132415

  7. Contractile actin cables induced by Bacillus anthracis lethal toxin depend on the histone acetylation machinery.

    Science.gov (United States)

    Rolando, Monica; Stefani, Caroline; Doye, Anne; Acosta, Maria I; Visvikis, Orane; Yevick, Hannah G; Buchrieser, Carmen; Mettouchi, Amel; Bassereau, Patricia; Lemichez, Emmanuel

    2015-10-01

    It remains a challenge to decode the molecular basis of the long-term actin cytoskeleton rearrangements that are governed by the reprogramming of gene expression. Bacillus anthracis lethal toxin (LT) inhibits mitogen-activated protein kinase (MAPK) signaling, thereby modulating gene expression, with major consequences for actin cytoskeleton organization and the loss of endothelial barrier function. Using a laser ablation approach, we characterized the contractile and tensile mechanical properties of LT-induced stress fibers. These actin cables resist pulling forces that are transmitted at cell-matrix interfaces and at cell-cell discontinuous adherens junctions. We report that treating the cells with trichostatin A (TSA), a broad range inhibitor of histone deacetylases (HDACs), or with MS-275, which targets HDAC1, 2 and 3, induces stress fibers. LT decreased the cellular levels of HDAC1, 2 and 3 and reduced the global HDAC activity in the nucleus. Both the LT and TSA treatments induced Rnd3 expression, which is required for the LT-mediated induction of actin stress fibers. Furthermore, we reveal that treating the LT-intoxicated cells with garcinol, an inhibitor of histone acetyl-transferases (HATs), disrupts the stress fibers and limits the monolayer barrier dysfunctions. These data demonstrate the importance of modulating the flux of protein acetylation in order to control actin cytoskeleton organization and the endothelial cell monolayer barrier. PMID:26403219

  8. Formation of mitochondrial apparatus of contractile cardiomyocytes during normal and hypoxic injury of cardi-ogenesis

    Directory of Open Access Journals (Sweden)

    Ivanchenko M.V.

    2013-01-01

    Full Text Available Changes of cardiomyocytes mitochondrial apparatus can be marked as the main factors which are the basis of various forms of cardiovascular disease, but the dynamics of morphogenetic rearrangements heart mitochondria are poorly researched under normal conditions and under the influence of harmful factors. Mitochondria of contractile cardiomyocytes are different in their morphology and localization in the cell, the biochemical properties and are able to form differently association with other intracellular structures. Question of the relationship between function and heterogeneity of regional specialization of mitochondria and the realization of the heterogeneity in the cell and the degree of their dependence on the disease during ontogeny is important and relevant. There are relatively few ultrastructural studies that investigate adaptive techniques and alternative processes in the mitochondria of atrial and ventricular myocardium under prenatal hypoxia during the development of the myocardium. It is interesting to find mechanisms for the implementation of the ultrastructural changes in the mitochondrial apparatus and extracellular tissue levels in hypoxic conditions on the stages of ontogeny.

  9. Evaluation of left ventricular contractility in anterior old myocardial infarction using MRI

    International Nuclear Information System (INIS)

    Currently, interventional therapy is often performed for coronary artery disease. Therefore accurate diagnosis of myocardial viability is indispensable. One diagnostic method is measurement of systolic myocardial wall thickening. In the present study, we evaluated myocardial viability in terms of segmental wall thickening using MRI. We performed spin-echo MRI and cine MRI using the double oblique method in 20 patients with anterior old myocardial infarction to obtain the left ventricular long-axis view and four-chamber view. We found a good correlation (r=0.84) between the left ventricular long-axis view and four-chamber view. We found a good correlation (r=0.84) between the left ventricular ejection fraction and the mean percentage wall thickening. Wall thickening of the infarcted myocardium was classified into three patterns (hypokinesis, akinesis and dyskinesis). Akinetic segments were further divided into two classes: one where the wall was unchanged throughout systole and diastole, and the other where thickening was delayed after systole. The segments with delayed contraction showed accumulation of fluorine-18-labeled fluorodeoxyglucose (FDG) by positron emission tomography (PET) or normalized wall motion after PTCA. Therefore, we considered that the segment showing delayed contraction was visible, even if a constant defect was evident on the 201Tl scintigram. In conclusion, analysis of wall thickening using MRI is very useful for evaluating left ventricular contractility and viability. (author)

  10. Simulation of the contractile response of cells on an array of micro-posts.

    LENUS (Irish Health Repository)

    McGarry, J P

    2009-09-13

    A bio-chemo-mechanical model has been used to predict the contractile responses of smooth cells on a bed of micro-posts. Predictions obtained for smooth muscle cells reveal that, by converging onto a single set of parameters, the model captures all of the following responses in a self-consistent manner: (i) the scaling of the force exerted by the cells with the number of posts; (ii) actin distributions within the cells, including the rings of actin around the micro-posts; (iii) the curvature of the cell boundaries between the posts; and (iv) the higher post forces towards the cell periphery. Similar correspondences between predictions and measurements have been demonstrated for fibroblasts and mesenchymal stem cells once the maximum stress exerted by the stress fibre bundles has been recalibrated. Consistent with measurements, the model predicts that the forces exerted by the cells will increase with both increasing post stiffness and cell area (or equivalently, post spacing). In conjunction with previous assessments, these findings suggest that this framework represents an important step towards a complete model for the coupled bio-chemo-mechanical responses of cells.

  11. Capacidade funcional e fatores associados em idosos: estudo populacional Capacidade funcional y factores asociados en ancianos: estudio poblacional Functional capacity and associated factors in the elderly: a population study

    Directory of Open Access Journals (Sweden)

    Roberta Souza Freitas

    2012-01-01

    Full Text Available OBJETIVO: Analisar a associação do comprometimento da capacidade funcional com condições de saúde e fatores sociodemográficos e comportamentais entre idosos residentes em comunidade do interior do Nordeste brasileiro. MÉTODOS: Estudo transversal de base populacional realizado com 316 idosos residentes em Lafaiete Coutinho-BA. A capacidade funcional foi avaliada por escala hierárquica, verificando associação com condições de saúde e fatores sóciodemográficos e comportamentais pela técnica de regressão logística multinomial. RESULTADOS: A dependência nas atividades independentes da vida diária (AIVD foi associada ao grupo etário > 80 anos, uso de dois ou mais medicamentos e comprometimento cognitivo. A dependência nas atividades básicas da vida diária e AIVD foi associada ao grupo etário > 80 anos, falta de participação em atividades religiosas, hospitalização nos últimos 12 meses, comprometimento cognitivo e sobrepeso. CONCLUSÃO: Houve associação com comprometimento da capacidade funcional e condições de saúde e fatores sociodemográficos entre idosos residentes em comunidade no Nordeste brasileiro.OBJETIVO: Analizar la asociación del compromiso de la capacidad funcional con condiciones de salud y factores sociodemográficos y comportamentales entre ancianos residentes en una comunidad del interior del Noreste brasileño. MÉTODOS: Estudio transversal de base poblacional realizado con 316 ancianos residentes en Lafaiete Coutinho-BA. La capacidad funcional fue evaluada por una escala jerárquica, verificando asociación con condiciones de salud y factores sociodemográficos y comportamentales por la técnica de regresión logística multinomial. RESULTADOS: La dependencia en las atividades independentes de la vida diaria (AIVD fue asociada al grupo etáreo > 80 años, uso de dos o más medicamentos y compromiso cognitivo. La dependencia en las actividades básicas de la vida diária y AIVD fue asociada al grupo

  12. Contractile Defect Caused by Mutation in MYBPC3 Revealed under Conditions Optimized for Human PSC-Cardiomyocyte Function.

    Science.gov (United States)

    Birket, Matthew J; Ribeiro, Marcelo C; Kosmidis, Georgios; Ward, Dorien; Leitoguinho, Ana Rita; van de Pol, Vera; Dambrot, Cheryl; Devalla, Harsha D; Davis, Richard P; Mastroberardino, Pier G; Atsma, Douwe E; Passier, Robert; Mummery, Christine L

    2015-10-27

    Maximizing baseline function of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) is essential for their effective application in models of cardiac toxicity and disease. Here, we aimed to identify factors that would promote an adequate level of function to permit robust single-cell contractility measurements in a human induced pluripotent stem cell (hiPSC) model of hypertrophic cardiomyopathy (HCM). A simple screen revealed the collaborative effects of thyroid hormone, IGF-1 and the glucocorticoid analog dexamethasone on the electrophysiology, bioenergetics, and contractile force generation of hPSC-CMs. In this optimized condition, hiPSC-CMs with mutations in MYBPC3, a gene encoding myosin-binding protein C, which, when mutated, causes HCM, showed significantly lower contractile force generation than controls. This was recapitulated by direct knockdown of MYBPC3 in control hPSC-CMs, supporting a mechanism of haploinsufficiency. Modeling this disease in vitro using human cells is an important step toward identifying therapeutic interventions for HCM. PMID:26489474

  13. The contractile vacuole in Ca2+-regulation in Dictyostelium: its essential function for cAMP-induced Ca2+-influx

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    Schlatterer Christina

    2006-06-01

    Full Text Available Abstract Background cAMP-induced Ca2+-influx in Dictyostelium is controlled by at least two non-mitochondrial Ca2+-stores: acidic stores and the endoplasmic reticulum (ER. The acidic stores may comprise the contractile vacuole network (CV, the endosomal compartment and acidocalcisomes. Here the role of CV in respect to function as a potential Ca2+-store was investigated. Results Dajumin-GFP labeled contractile vacuoles were purified 7-fold by anti-GFP-antibodies in a magnetic field. The purified CV were shown for the first time to accumulate and release Ca2+. Release of Ca2+ was elicited by arachidonic acid or the calmodulin antagonist W7, the latter due to inhibition of the pump. The characteristics of Ca2+-transport and Ca2+-release of CV were compared to similarly purified vesicles of the ER labeled by calnexin-GFP. Since the CV proved to be a highly efficient Ca2+-compartment we wanted to know whether or not it takes part in cAMP-induced Ca2+-influx. We made use of the LvsA--mutant expected to display reduced Ca2+-transport due to loss of calmodulin. We found a severe reduction of cAMP-induced Ca2+-influx into whole cells. Conclusion The contractile vacuoles in Dictyostelium represent a highly efficient acidic Ca2+-store that is required for cAMP-induced Ca2+-influx.

  14. The effects of space flight on the contractile apparatus of antigravity muscles: implications for aging and deconditioning

    Science.gov (United States)

    Baldwin, K. M.; Caiozzo, V. J.; Haddad, F.; Baker, M. J.; Herrick, R. E.

    1994-01-01

    Previous studies have shown that the unloading of skeletal muscle, as occurring during exposure to space flight, exerts a profound effect on both the mass (cross sectional area) of skeletal muscle fibers and the relative expression of protein isoforms comprising the contractile system. Available information suggests that slow (type I) fibers, comprising chiefly the antigravity muscles of experimental animals, in addition to atrophying, undergo alterations in the type of myosin heavy chain (MHC) expressed such that faster isoforms become concomitantly expressed in a sub-population of slow fibers when insufficient force-bearing activity is maintained on the muscle. Consequently, these transformations in both mass and myosin heavy chain phenotype could exert a significant impact on the functional properties of skeletal muscle as manifest in the strength, contractile speed, and endurance scope of the muscle. To further explore these issues, a study was performed in which young adult male rats were exposed to zero gravity for six days, following which, the antigravity soleus muscle was examined for a) contractile properties, determined in situ and b) isomyosin expression, as studied using biochemical, molecular biology, and histochemical/immunohistochemical techniques.

  15. Contractile Defect Caused by Mutation in MYBPC3 Revealed under Conditions Optimized for Human PSC-Cardiomyocyte Function

    Directory of Open Access Journals (Sweden)

    Matthew J. Birket

    2015-10-01

    Full Text Available Maximizing baseline function of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs is essential for their effective application in models of cardiac toxicity and disease. Here, we aimed to identify factors that would promote an adequate level of function to permit robust single-cell contractility measurements in a human induced pluripotent stem cell (hiPSC model of hypertrophic cardiomyopathy (HCM. A simple screen revealed the collaborative effects of thyroid hormone, IGF-1 and the glucocorticoid analog dexamethasone on the electrophysiology, bioenergetics, and contractile force generation of hPSC-CMs. In this optimized condition, hiPSC-CMs with mutations in MYBPC3, a gene encoding myosin-binding protein C, which, when mutated, causes HCM, showed significantly lower contractile force generation than controls. This was recapitulated by direct knockdown of MYBPC3 in control hPSC-CMs, supporting a mechanism of haploinsufficiency. Modeling this disease in vitro using human cells is an important step toward identifying therapeutic interventions for HCM.

  16. CAPACIDADE DE DESENVOLVER TRABALHO FÍSICO PROGRESSIVO EM CONDIÇÕES DE HIPOTERMIA BRANDA

    Directory of Open Access Journals (Sweden)

    Bárbara Regina Alvarez

    2008-07-01

    Full Text Available O propósito deste estudo determinar, em 12 voluntários do Grupo de Busca e Salvamento da Polícia Militar de Santa Catarina, a capacidade de desenvolver trabalho físico na bicicleta ergométrica (cargas escalonadas de 50, 100 e 150 watt em condições ambientais neutras (28 graus e subnormal (20 graus, com varredura ampliada da temperatura cutânea (7 sensores periféricos, da temperatura central (sensor piloto e da temperatura ambiental (sensor referência, a estimativa do VO2 máximo foi efetuada com auxílio do nomograma desenvolvido por Astrand (1960, empregando um cicloergômetro de frenagem magnética da FUNBEC. Já o % de mobilização do VO2 max foi predito pela equação proposta por Katch (1978, empregando o % da mobilidade da FC. Os dados foram tratados através da ferramenta eletrônica MICROSTAT, empregando o artifício estático "t" de student monocaudal. Foi observada uma variação significativa (P < 0.05 na temperatura de repouso entre as duas condições (%= -0.52. Considerando a estimativa do valor padrão (3 watt/Kg, a PWC170 apresentou uma diferença significativa entre médias (%=-14.29. A sobreposição dos pontos térmicos evidenciou um marcado estado de equilíbrio na FC nos minutos finais de cada desempenho biológico, corroborando a hipótese de ajustamento imediato dos sistemas cardiorrespiratório e metabólico para atender a demanda tecidual de oxigênio e nutrientes. Para estudos complementares, sugerimos a utilização da banda inferior da zona hipotérmica (flutuação de 20 a 5 graus Celsius para que o impacto da condição subnormal produza alterações mais importantes na temperatura central.

  17. Fenólicos totais e capacidade antioxidante in vitro de polpas de frutos tropicais

    Directory of Open Access Journals (Sweden)

    Luanne Morais Vieira

    2011-09-01

    Full Text Available O consumo de frutos e suas polpas tem sido muito recomendado por seu valor nutricional, alto teor de fibras, vitamina C e carotenoides. Trabalhos recentes têm apontado esses alimentos como fontes de compostos fenólicos com ação antioxidante, portanto sequestradores de radicais livres, com ação protetora contra o surgimento e/ou desenvolvimento de processos degenerativos que conduzem a doenças crônicas não transmissíveis. Devido à crescente comercialização e consumo de polpas de frutas no Brasil, especialmente na cidade de Teresina-Piauí, este trabalho selecionou um grupo de polpas de frutos de elevado consumo local para avaliação do teor de fenólicos totais e da atividade antioxidante in vitro pelo método de captura de radicais livres: DPPH (radical 1,1-diphenil-2-picrilhydrazil e ABTS (radical 2,2'azinobis(3-ethylbenzthiazoline-6-sulfonic acid. Os frutos selecionados foram: Acerola (Malpighia emarginata DC., Bacuri (Platonia insignis Mart., Cajá (Spondias mombin L., Caju (Anacardium occidentale, Goiaba(Psidium guajava e Tamarindo (Tamarindus indica L.. Os teores de fenólicos totais encontrados nas polpascongeladas destes frutos exibiram quantidades relevantes de polifenóis, destacando-se a polpa de acerola com 835,25 ± 32,44 e 449,63 ± 10,24 mg /100g nos extratos aquosos e hidroalcoólicos, respectivamente, seguido pela polpa de caju com 201,61 ± 19,15 e 165,07 ± 4,10 mg /100g. As polpas de bacuri e tamarindo foram as que apresentaram os menores teores de fenólicos totais. Com relação à atividade antioxidante in vitro, os melhores resultados foram encontrados para os extratos aquosos e hidroalcoólicos das polpas de acerola, caju e goiaba. A capacidade antioxidante destas polpas (EC50 em µg/mL variou de 24,42 a 413,36 e de 1,74 a 259,18 para os extratos aquosos e hidroalcoólicos, respectivamente. Utilizando o radical ABTS, a atividade antioxidante para essas mesmas polpas de frutas apresentou valores TEAC que

  18. Cómo mejorar la capacidad inferencial en estudiantes universitarios Como melhorar a capacidade inferencial em estudantes universitários How to Improve the Inferential Ability of College Students

    OpenAIRE

    Mireya Cisneros-Estupiñán; Giohanny Olave-Arias; llene Rojas-García

    2012-01-01

    La investigación titulada "Mejoramiento de la capacidad inferencial en estudiantes universitarios" se propuso diseñar estrategias didácticas para desarrollarla comprensión lectora de textos académicos entre estudiantes. Se trabajó bajo la modalidad de proyecto factible, con intervención de pilotajes en la fase de desarrollo. El principal hallazgo es la propuesta estructurada, gradual y adaptable de un programa de entrenamiento en lectura comprensiva para los programas universitarios y sus cur...

  19. Chaperonin containing T-complex polypeptide subunit eta (CCT-eta is a specific regulator of fibroblast motility and contractility.

    Directory of Open Access Journals (Sweden)

    Latha Satish

    Full Text Available Integumentary wounds in mammalian fetuses heal without scar; this scarless wound healing is intrinsic to fetal tissues and is notable for absence of the contraction seen in postnatal (adult wounds. The precise molecular signals determining the scarless phenotype remain unclear. We have previously reported that the eta subunit of the chaperonin containing T-complex polypeptide (CCT-eta is specifically reduced in healing fetal wounds in a rabbit model. In this study, we examine the role of CCT-eta in fibroblast motility and contractility, properties essential to wound healing and scar formation. We demonstrate that CCT-eta (but not CCT-beta is underexpressed in fetal fibroblasts compared to adult fibroblasts. An in vitro wound healing assay demonstrated that adult fibroblasts showed increased cell migration in response to epidermal growth factor (EGF and platelet derived growth factor (PDGF stimulation, whereas fetal fibroblasts were unresponsive. Downregulation of CCT-eta in adult fibroblasts with short inhibitory RNA (siRNA reduced cellular motility, both basal and growth factor-induced; in contrast, siRNA against CCT-beta had no such effect. Adult fibroblasts were more inherently contractile than fetal fibroblasts by cellular traction force microscopy; this contractility was increased by treatment with EGF and PDGF. CCT-eta siRNA inhibited the PDGF-induction of adult fibroblast contractility, whereas CCT-beta siRNA had no such effect. In each of these instances, the effect of downregulating CCT-eta was to modulate the behavior of adult fibroblasts so as to more closely approximate the characteristics of fetal fibroblasts. We next examined the effect of CCT-eta modulation on alpha-smooth muscle actin (alpha-SMA expression, a gene product well known to play a critical role in adult wound healing. Fetal fibroblasts were found to constitutively express less alpha-SMA than adult cells. Reduction of CCT-eta with siRNA had minimal effect on cellular

  20. CAPACIDADE DO SISTEMA DE LEVANTE HIDRÁULICO DOS TRATORES AGRÍCOLAS DE PNEUS NO BRASIL

    Directory of Open Access Journals (Sweden)

    André Augusto Veit

    2015-12-01

    Full Text Available O sistema hidráulico do trator se torna importante por ser um modo de acoplamento de alguns implementos agrícolas, além de transmitir potência. Por isso, objetivou-se verificar as variações da potência do motor relacionadas com a capacidade de levante dos três pontos, pressão máxima e vazão máxima da bomba. Também observar a capacidade de levante em função da massa do trator sem lastro. Para isto, foram coletados dados de 171 modelos de 11 marcas diferentes de tratores agrícolas de pneus. Estes foram classificados pela potência do motor segundo a ANFAVEA (Associação nacional dos fabricantes de veículos automotores. Foi construída uma planilha eletrônica para coletar as variáveis a serem estudadas. A vazão máxima da bomba foi a variável a qual menos foram encontrados dados, variando de 39,13 a 62,16% nas diferentes classes. Tratores da classe II e III apresentaram as melhores relações quando uma das variáveis era capacidade de levante. A variação média da vazão máxima da bomba relacionada à potência motora é proporcional, ou seja, tanto a vazão quanto a potência se elevaram igualmente, com valores médios de 0,79 até 1,09, nas diferentes classes.

  1. Biolixiación y biooxidación de minerales utilizando cepas nativas con capacidad termofílica

    OpenAIRE

    Giaveno Filippa, María Alejandra

    2010-01-01

    El Objetivo General de este Trabajo de Tesis cubre un aspecto que se considera relevante tanto desde el punto de vista básico como aplicado. El mismo consiste en la búsqueda de consorcios extremófilos dentro del Campo Geotermal Copahue, el análisis de sus potencialidades lixiviantes medido de acuerdo a las capacidades de oxidar hierro(II) y azufre elemental y su posterior uso en procesos de biolixiviación y biooxidación sobre minerales de interés esencialmente regional. En el caso de la bioli...

  2. Innovación, capacidad productiva, formación en el puesto de trabajo y productividad

    OpenAIRE

    Guisado González, Manuel; Vila Alonso, Mercedes; Guisado Tato, Manuel

    2016-01-01

    [ES] Este estudio analiza la relación entre productividad laboral, innovación radical, innovación incremental, tecnología incorporada en maquinaria y equipos, utilización de la capacidad productiva y formación. Los datos utilizados son de empresas españolas, manufactureras y de servicios, procedentes de la Business Environment and enterprise Performance Survey (BEEPS). La técnica empleada para estimar los coeficientes del modelo es la regresión mínimo cuadrática ordinaria, ya que la variable ...

  3. Revisión sobre la capacidad de repetir sprint o RSA en jugadores de fútbol

    OpenAIRE

    Saenz Tomás, Juan

    2014-01-01

    En los últimos años, la capacidad para repetir esprines (RSA), se ha considerado como un factor limitante del rendimiento en el contexto deportivo del fútbol. En este sentido, esta revisión analiza los aspectos importantes que condicionan el rendimiento de la RSA, tales como los aspectos fisiológicos, los diferentes métodos de entrenamiento que se utilizan así como los test más utilizados para su evaluación. Por ello, el objetivo principal de esta revisión es dar a conocer los métodos y recom...

  4. Capacidad predictiva de la autoeficacia académica sobre las dimensiones del autoconcepto en una muestra de adolescentes chilenos

    OpenAIRE

    García-Fernández, J.M.; Díaz-Herrero, A. (Ángela); Inglés, C.J. (Cándido J.); Torregrosa, M.S. (María Soledad); Lagos-San-Martín, N. (Nelly); Gonzálvez, C. (Carolina)

    2016-01-01

    El objetivo de este estudio fue analizar la capacidad predictiva de la autoefi cacia académica sobre las dimensiones del autoconcepto en una muestra de 860 estudiantes chilenos. El análisis de regresión logística reveló que la autoefi cacia académica fue un predictor positivo y signifi cativo de las escalas académicas (Matemáticas, Verbal y Académica General), no académicas (Habilidades Físicas, Apariencia Física, Relaciones con el Sexo Opuesto, Relaciones con el Mi...

  5. LAS CAPACIDADES TECNOLÓGICAS COMO BASE PARA EL DESARROLLO (TECHNOLOGICAL CAPABILITIES AS A BASIS FOR DEVELOPMENT

    Directory of Open Access Journals (Sweden)

    Carvajal Villaplana Álvaro

    2010-04-01

    Full Text Available Resumen:En este artículo interesa determinar la estructura lógica del concepto de capacidades tecnológicas para establecer las características y los componentes más relevantes, esto con la intención de proponer una definición filosófica a partir de las ideas de capacidades y funcionamientos de Amartya Sen y del concepto de disposiciones de Gilbert Ryle. Se trata de una ampliación o aplicación de esas nociones a la idea de capacidades tecnológicas con el fin de obtener dicho concepto operativo y apropiado. El estudio se lleva a cabo, porque la noción de capacidades tecnológicas es muy utilizada en diversos textos de economía, administración, tecnología y filosofía, pero sin que se las defina apropiadamente ni se les fundamente filosóficamente. La investigación en la que se sustenta el artículo se realizó en el año 2005 con un enfoque de análisis inscrito en la tradición filosófica analítica.Abstract:In this work I am interested in determining the logical structure of the concept of technological capabilities to establish their characteristics and components. This is done with the intention of proposing an operative definition of such a notion. For this purpose I proceed to establish its theoretical foundation, inspired in Amartya Sen’s conception of capability and operation, as well as in Ryle's concept of disposition. It is an amplification or application from those notions to the idea of technological capability in order to obtain an operative and appropriate notion. An exploration of the notion of tecnological capabilities seems very timely because of its widespread use in economics, business administration and philosophy, areas in which both a clear definition and a theoretical explanation are usually missing. The research for this paper took place in 2005, when its author was working for his Ph.D. at the Carlos III University in Madrid. Our approach here is typical of the analytical tradition in philosophy.

  6. Capacidad de Vibrio fluvialis (LEE, 1981) para producir infección en pez dorado (Carassius auratus. L)

    OpenAIRE

    Pilar Negrete Redondo; Jorge Romero Jarero; José Luis Arredondo Figueroa

    2004-01-01

    A algunos peces de ornato de la especie Carassius auratus se les inyectó, vía intramuscular, diferentes dosis infectivas de la bacteria Vibrio fluvialis con el fin de probar la capacidad de ésta para producir infección en organismos acuáticos, establecer su correspondiente D50L y determinar si esta bacteria puede infectar los individuos cultivados a través del agua de los estanques de cultivo, o si necesita de un hospedero vivo para trasmitir y producir infección. Se estableció la relación et...

  7. A influência da cor nos desenhos dos alunos : um mecanismo para o desenvolvimento das suas capacidades

    OpenAIRE

    Jácome, Mara Cristina Gonçalves

    2011-01-01

    Relatório de estágio de mestrado em Ensino de Educação Visual e Tecnológica no Ensino Básico Este trabalho teve como principal objetivo estudar "A Influência da cor nos desenhos dos alunos: um mecanismo para o desenvolvimento das suas capacidades". Os principais temas abordados foram o círculo cromático da cor, simbologia da cor e a banda desenhada. Todo o projeto foi desenvolvido numa Escola EB2/3 em Braga com uma turma do 6.º ano, no âmbito da disciplina de Educação Visual e ...

  8. RELACIÓN DEL SISTEMA FAMILIAR Y CAPACIDAD DE AUTOCUIDADO DEL ADOLESCENTE QUE CONSUME BEBIDAS ALCOHÓLICAS

    OpenAIRE

    Lidia García Ortiz; Leticia Casique Casique

    2012-01-01

    El consumo de alcohol se inicia en la etapa adolescente y puede propiciar múltiples problemas, como el bajo rendimiento escolar, abandono de sus estudios, embarazos no deseados, dificultades familiares y accidentes automovilísticos, que los puede llevar a la muerte. Además el consumo de alcohol puede ser la puerta de entrada a otro tipo de adicciones. (Instituto Nacional de Estadística, 2006) Analizar la relación del Sistema Familiar y la Capacidad de Autocuidado del adolescente, ayuda a enfe...

  9. La internacionalización de la empresa a través del enfoque de recursos y capacidades.

    OpenAIRE

    Jiménez Palmero, Alfredo

    2008-01-01

    La internacionalización de la empresa constituye un fenómeno de especial relevancia dadas las controvertidas repercusiones que las actividades de las empresas multinacionales tienen tanto en su país de origen como de destino. El enfoque de recursos y capacidades ha sido un marco teórico muy empleado en la investigación científica para tratar de explicar el porqué y el cómo de esta importante decisión estratégica. El presente trabajo pretende ofrecer una revisión bibliográfica de algunos de lo...

  10. Capacidade materna de cuidar e desnutrição infantil Mother's ability of childcare and children malnutrition

    OpenAIRE

    Maria Antonieta de BL Carvalhaes; Maria Helena D'Aquino Benício

    2002-01-01

    OBJETIVOS: Identificar e medir a magnitude do risco de desnutrição associada a fatores determinantes da capacidade materna de cuidado infantil: estrutura familiar, escolaridade, trabalho, saúde física e saúde mental maternas. MÉTODOS: Delineou-se um estudo de casos e controles. Foram selecionados 101 casos (crianças com peso/idade abaixo do percentil 5) e 200 controles (crianças com peso/idade acima do percentil 25) mediante inquéritos antropométricos realizados durante três "Dias Nacionais d...

  11. DIFERENCIAS EN CAPACIDAD VISUAL, MEMORIA E INTELIGENCIAS MÚLTIPLES ENTRE ALUMNOS DE DIVERSIFICACIÓN CURRICULAR Y NORMALIZADOS

    OpenAIRE

    Gardoki-Lamikiz, Edortza

    2013-01-01

    En el presente estudio se propuso determinar la posible existencia de diferencias en capacidad visual, memoria e inteligencias múltiples entre el alumnado del Programa de Diversificación Curricular y el alumnado de grupos normalizados. El estudio se llevo a cabo con 44 alumnos de grupos de diversificación curricular y grupos normalizados con edades comprendidas entre los 14 y 17 años, analizándose la velocidad lectora, los movimientos sacádicos, los aspectos de la lectura, las ...

  12. CAPACIDAD DE LA MEMORIA DE TRABAJO VERBAL. VALIDEZ Y FIABILIDAD DE UNA TAREA DE AMPLITUD DE LECTURA

    OpenAIRE

    JUAN PABLO BARREYRO; DÉBORA I. BURIN; D. ANÍBAL DUARTE

    2009-01-01

    La memoria de trabajo se refiere a todos aquellos mecanismos o procesos implicados en el control, regulación y mantenimiento activo de información relevante para la ejecución de ta reas cognitivas complejas (Miyake & Shah, 1999); es uno de los principales factores limitantes de las capacidades de alto nivel jerárquico, como el razonamiento o la comprensión lectora. En este trabajo se informa un estudio en el que se analizaron las propiedades psicométricas de una de las tareas más co nocidas p...

  13. O uso do Índice de Barthel Modificado em idosos: contrapondo capacidade funcional, dependência e fragilidade

    OpenAIRE

    Juliana Balbinot Reis Girondi; Karina Silveira de Almeida Hammerschimidt; Francisco Reis Tristão; Darla Lusia Ropelato Fernandez

    2014-01-01

    Introdução: Estudo transversal descritivo realizado entre junho e dezembro de 2010. Objetivo: avaliar a capacidade funcional de um grupo de idosos para conhecer e refletir sobre essa relação com a fragilidade e dependência. Métodos: Envolveu 18 idosos com deficiência física cadastrados em duas unidades de Saúde da Família de Florianópolis. Utilizou-se entrevista, aplicação do Mini Exame do Estado Mental e Índice de Barthel Modificado. Resultados: Aplicando-se estatística descritiva, identific...

  14. Integración de decisiones de inversión en capacidad en la planificación agregada

    OpenAIRE

    Corominas Subias, Albert; Lusa García, Amaia; Martínez Costa, Carme

    2010-01-01

    En este trabajo se presenta un modelo de programación lineal mixta para un problema de planificación agregada en el que se incluyen decisiones de ampliación de la capacidad que suelen inscribirse en un nivel de planificación estratégica. Se supone que el equipo adicional (que puede adquirirse o no) y el antiguo se integran para dar lugar a una nueva configuración productiva única, con costes variables distintos de los correspondientes al equipo antiguo. El modelo, cuyo objetivo co...

  15. ¿SON LOS PADRES Y PROFESORES BUENOS DETECTORES DE LAS ALTAS CAPACIDADES INTELECTUALES DE LOS ALUMNOS?

    OpenAIRE

    Fernández-Sánchez, Inmaculada

    2013-01-01

    Los alumnos con altas capacidades intelectuales son una población con posibles dificultades adaptativas a la escuela, ya que un porcentaje elevado de ellos presentan un síndrome de bajo rendimiento, debido, entre otras cosas, a sus características psiconeurológicas y a la falta de formación del profesorado para identificarlos y para intervenir con las medidas educativas apropiadas. Este trabajo tiene como objetivo conocer la adecuación de los procesos de identificación de al...

  16. Experiencia de trabajo en una asociación de apoyo extracurricular a alumnos de altas capacidades

    OpenAIRE

    Tomás Tomás, Jorge

    2015-01-01

    Treball final de Màster Universitari en Professor/a d'Educació Secundària Obligatòria i Batxillerat, Formació Professional i Ensenyaments d'Idiomes. Codi: SAP129. Curs acadèmic 2014-2015 Como paso final del Máster Universitario en Profesorado de Educación Secundaria Obligatoria y Bachiller, Formación Profesional y Enseñanza de Idiomas plasmamos en negro sobre blanco la esencia vivida en una Asociación de apoyo extracurricular para alumnos con Altas Capacidades con la reaIización del presen...

  17. Atención a los alumnos con altas capacidades en la educación secundaria

    OpenAIRE

    Caballero-Santamaria, Raquel

    2013-01-01

    El presente Trabajo Fin de Máster pretende informar de los resultados obtenidos en un trabajo de investigación consistente en el estudio del tratamiento que se da a los alumnos con altas capacidades en distintos centros educativos de la ciudad de Barcelona, cuales son los métodos de detección, los recursos que se les destinan y su nivel de concienciación. El desarrollo de esta investigación se ha basado en una fundamentación bibliográfica entorno a los principios que afectan al alumnado co...

  18. Indicio de altas capacidades en Educación Infantil. Propuesta de un programa de enriquecimiento curricular

    OpenAIRE

    Vílchez García, Natalia

    2016-01-01

    El presente documento pretende dar respuesta a una realidad educativa que por estadística todo maestro a lo largo de su docencia tendrá frente sí. Este es el caso de alumnos con altas capacidades intelectuales. Abogando por el fin último de la educación, os presento brevemente cómo poder detectar y ayudar a dichos niños desde edades muy tempranas. Por otro lado, propongo un programa de enriquecimiento curricular que se ofrece como estrategia educativa para estimular la motivación y favorecer ...

  19. Electroestimulación neuromuscular intradiálisis, fuerza muscular, capacidad funcional y composición corporal

    OpenAIRE

    Sandra Rubio Páez; Vicent Esteve Simó; Anna Junqué Jiménez; Ester Tomás Bernabéu; Oscar Paz López; Gorka Iza Pinedo; María Luisa Lavado Sempere; Manel Ramírez de Arellano

    2015-01-01

    Introducción: La capacidad funcional disminuida y la importante atrofia muscular caracterizan a los pacientes en hemodiálisis (HD). El ejercicio físico intradiálisis y recientemente la electroestimulación neuromuscular (EMS), representan dos serias opciones terapéuticas para mejorar esta deteriorada condición física. Actualmente, no existen estudios publicados sobre el papel de la EMS y la composición corporal en los pacientes en HD. Objetivo: Analizar que efecto produce un programa de EMS so...

  20. EL MICROCRÉDITO COMO INSTRUMENTO DE SUPERACIÓN DE LA POBREZA Y FORTALECIMIENTO DE CAPACIDADES EN COLOMBIA

    OpenAIRE

    Manrique-Joya, Gloria Mercedes; Guzmán-Silva, Clara Patricia

    2015-01-01

    Los crecientes niveles de desigualdad social y pobreza que en la actualidad se evidencian globalmente, no sólo en países en vías de desarrollo sino también en aquellos considerados desarrollados, reflejan la imperante necesidad de crear políticas incluyentes, que dejen de lado enfoques utilitaristas, los cuales, generalmente, reducen el bienestar social a un incremento de los ingresos en una forma que sea suficiente para satisfacer las necesidades básicas, dejando de lado las capacidades del ...