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Sample records for cancer du col

  1. Cervix cancer; Cancer du col uterin

    Energy Technology Data Exchange (ETDEWEB)

    Pointreau, Y.; Ruffier Loubiere, A.; Barillot, I. [Service de radiotherapie, centre regional universitaire de cancerologie Henry-S.-Kaplan CHU de Tours, Hpital Bretonneau, 37 - Tours (France); Pointreau, Y. [Universite Francois-Rabelais de Tours, GICC, 37 - Tours (France); CNRS, UMR 6239 -Genetique, Immunotherapie, Chimie et Cancer-, 37 - Tours (France); CHRU de Tours, laboratoire de pharmacologie-toxicologie, 37 - Tours (France); Denis, F. [Centre Jean-Bernard, 72 - Le Mans (France); Barillot, I. [Universite Francois-Rabelais, 37 - Tours (France)

    2010-07-01

    Cervix cancers declined in most developed countries in recent years, but remain, the third worldwide leading cause of cancer death in women. A precise staging, based on clinical exam, an abdominal and pelvic MRI, a possible PET-CT and a possible lymph node sampling is necessary to adapt the best therapeutic strategy. In France, the treatments of tumors of less than 4 cm without nodal involvement are often based on radiotherapy followed by surgery and, whereas tumors larger than 4 cm and involved nodes are treated with concurrent chemoradiotherapy. Based on an illustrated clinical case, indications, delineation, dosimetry and complications expected with radiotherapy are demonstrated. (authors)

  2. Pertinence du dépistage du cancer du col de l'utérus au sein d'un établissement de santé

    OpenAIRE

    Chignier, Pauline

    2014-01-01

    Le dépistage du cancer du col de l'utérus est un enjeu de Santé Publique. Le plan cancer 2014-2019 souligne l'importance de réduire les inégalités face au dépistage et de couvrir 80% de la population cible. L'objectif principal de l'étude était d'évaluer la conformité des pratiques cliniques des professionnels de santé aux recommandations de la HAS. L'objectif secondaire était d'évaluer la qualité du dépistage du cancer du col selon le type de suivi : gynécologique versus obstétrical. Il s'ag...

  3. Prise en charge du cancer du col utérin durant la grossesse: à propos de 05 cas

    OpenAIRE

    El Mazghi, Abderrahman; Bouhafa, Touria; Loukili, Kaoutar; El Kacemi, Hanan; Lalya, Issam; Hassouni, Khalid

    2014-01-01

    L'association d'un cancer du col utérin avec une grossesse est une éventualité rare. Son incidence est assez mal évaluée, elle se situe selon les études entre 1 et 2/10 000. Lorsque la découverte en est faite, il faut conjuguer deux impératifs parfois totalement divergents: le traitement de la mère et la prise en charge du foetus. Cette association pose schématiquement quatre grands problèmes, qui sont: Celui du diagnostic, qui est loin d’être évident, compte tenu des remaniements cervicaux o...

  4. Radiotherapy in the treatment of a locally advanced cervical cancer: experience of the Casablanca Radiotherapy-Oncology centre; Radiotherapie dans le traitement du cancer du col uterin localement evolue: experience du centre de radiotherapie-oncologie de Casablanca

    Energy Technology Data Exchange (ETDEWEB)

    Bouchbika, Z.; Benchakroun, N.; Sellal, N.; Jouhadi, H.; Tawfiq, N.; Sahraoui, S.; Benider, A. [Service radiotherapie-oncologie, CHU Ibn-Rochd, Casablanca (Morocco)

    2011-10-15

    The authors report the assessment of the local control and relapse rates in a set of 70 patients who had been treated in 2004 and 2005 by radiotherapy associated or not with chemotherapy for a cervical cancer of stage IIbd, III or IVa according to the FIGO classification. The obtained results correspond to that published in literature. The authors expect that the improvement of radiotherapy techniques could improve these results. Short communication

  5. Brachytherapy in cervix cancers: techniques and concepts evolution; Curietherapie dans les cancers du col uterin: evolution des techniques et des concepts

    Energy Technology Data Exchange (ETDEWEB)

    Haie-Meder, C.; Crevoisier, R. de; Petrow, P.; Fromm, S.; Delapierre, M.; Albano, M.; Petit, C.; Briot, E. [Institut Gustave Roussy, 94 - Villejuif (France). Service de Curietherapie

    2003-02-01

    Brachytherapy plays an important role in the treatment of patients with cervical carcinoma. Technical modalities have evolved during the last years and have benefited from imaging modalities development, specially MRI. Imaging modalities contribute to a better knowledge of tumoral extension and critical organs. Ultrasound during brachytherapy has led to the almost complete eradication of uterine perforation. In the future, a more systematic use of systems allowing optimization may induce a better dose distribution in the tumor as well as in the critical organs. Recent data provided information in favor of a better analysis in the relative role of dose-rate, total dose and treated volume and their influence on the local control and complication incidence. Concomitant radio-chemotherapy represents a standard in the treatment of patients with tumoral size exceeding 4 cm. Some questions still remain: is concomitant chemotherapy of benefit during brachytherapy? Is there any place for complementary surgery, specially in patients with complete response after external irradiation with concomitant chemotherapy and brachytherapy? In order to answer the former question, a phase III randomized trial is going to start, with the Federation Nationale des Centres de Lutte Contre le Cancer as a promoter. (authors)

  6. Cervix carcinomas: place of intensity-modulated radiotherapy; Les cancers du col uterin: place de la radiotherapie avec modulation d'intensite

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    Barillot, I. [Centre Regional Universitaire de Cancerologie H.S.-Kaplan, Hopital Bretonneau, CHU de Tours, 37 - Tours (France); Universite Francois-Rabelais, 37 - Tours (France)

    2009-10-15

    While indications of modulated intensity radiation therapy (I.M.R.T.) are perfectly defined in head and neck and prostate cancer patients, this technique remains under evaluation for gynecologic tumours. The implementation of conformal three dimensional radiotherapy in the late 1990 has been the first important step for optimisation of treatment of cervix carcinomas, as it permitted a better target coverage with a significant reduction of the bladder dose. However, this technique often leads to an irradiation of a larger volume of rectum in locally advanced stages and could only spare a limited amount of intestine. I.R.M.T. is one of the optimisation methods potentially efficient for a better sparing of digestive tract during irradiation of cervix carcinomas. The aim of this literature review is to provide the arguments supporting this hypothesis, and to define the place of this technique for dose escalation. (authors)

  7. Evidence based of chemoradiotherapy in cervix carcinoma; Chimioradiotherapie concomitante dans les cancers du col de l'uterus: quels niveaux de preuve?

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    Joly-Lobbedez, F. [CHU de la Cote-de-Nacre, 14 - Caen (France); CLCC Francois-Baclesse, Oncologie Medicale, 14 - Caen (France)

    2009-10-15

    Since 10 years, the combination of chemoradiotherapy has become a standard of treatment of the advanced localized cervical cancer. Two systematic reviews of the literature (including the results of the different clinical trials) have already been published. The aim of this article is to present the results of the recent meta-analysis based on individual patient data and to discuss the perspectives. This meta-analysis was rigorously designed: trials selected had the same control arm with the same radiotherapy without concomitant chemotherapy, the definition of the primary outcome (overall survival) was homogeneous and analysis was made in intent to treat. The results confirm the advantage in overall survival in favor of the chemoradiotherapy with an absolute 5-year overall survival benefit of 6% (60-66%) and 8% of 5-year disease-free survival (50-58%). Interestingly, even if cisplatin seems to be the most active drug, a significant advantage is also observed with no platinum chemotherapy. A polychemotherapy is not more active than a mono chemotherapy and there was a suggestion of a difference in the size of the survival benefit with tumor stage. Larger benefits were seen for the few trials in which additional chemotherapy was administered after chemoradiotherapy, but results have to be confirmed by other clinical trials. Late toxicity was not well evaluated and a long-term follow-up of the patients is important to assess the real incidence of long-term side effects of the chemoradiotherapy and the impact on quality of life. New strategies combining new chemotherapy protocols or targeted therapy with radiation are promising but have to be evaluated in comparative clinical trials before use in routine. (authors)

  8. Métastase cérébrale d'un carcinome du col utérin

    Science.gov (United States)

    Chekrine, Tarik; Hassouni, Abdesalam; Jouhadi, Hassan; Sahraoui, Souha; Bouchbika, Zineb; Taleb, Amina; Benchakroun, Nadia; Tawfiq, Nezha; Benider, Abdellatif

    2013-01-01

    Les métastases cérébrales des cancers du col de l′utérus sont extrêmement rares. Elles sont généralement supra-tentorielles, survenant à un stade avancé de la maladie et dans un cadre de néoplasie polymétastatique. La tumeur primitive est le plus souvent un carcinome épidermoïde peu différencié. Leur pronostic reste sombre malgré toutes les options thérapeutiques. Vu la rareté de cet événement et le peu de cas publiés dans la littérature, nous rapportons l'observation clinique d'une jeune patiente de 44 ans, opérée pour un carcinome du col utérin et qui présente 14 mois plus tard des métastases cérébrales sus et sous tentorielles associées à des métastases ganglionnaires lombo-aortique, médiastinale et sus-claviculaire. Elle a bénéficié d'un traitement palliatif associant une chimiothérapie et une radiothérapie pan encéphalique. Devant l'altération rapide de l'état général, la patiente a été mise sous un traitement symptomatique et des soins de support. PMID:23717727

  9. Positron emission tomography in the management of cervix cancer patients; Tomographie par emission de positons dans la prise en charge des cancers du col de l'uterus

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    Bonardel, G.; Gontier, E.; Soret, M.; Dechaud, C.; Fayolle, M.; Foehrenbach, H. [Hopital d' Instruction des Armees du Val-de-Grace, Service de Medecine Nucleaire, 75 - Paris (France); Chargari, C.; Bauduceau, O. [Hopital d' Instruction des Armees du Val-de-Grace, Service de Radiotherapie, 75 - Paris (France)

    2009-10-15

    Since its introduction in clinical practice in the 1990 s, positron emission tomography (PET), usually with {sup 18}F-fluoro-2-deoxy-D-glucose ({sup 18}F-F.D.G.), has become an important imaging modality in patients with cancer. For cervix carcinoma, F.D.G.-PET is significantly more accurate than computed tomography (CT) and is recommended for loco-regional lymph node and extra pelvic staging. The metabolic dimension of the technique provides additional prognostic information. Ongoing studies now concentrate on more advanced clinical applications, such as the planning of radiotherapy, the response evaluation after the induction of therapy, the early detection of recurrence. Technical innovations, such as PET cameras with better spatial resolution and hybrid positron emission tomography/computed tomography (PET-CT), available now on the whole territory, provide both anatomic and metabolic information in the same procedure. From the point of view of biological metabolism, new radiopharmaceutical probes are being developed. Those hold promise for future refinements in this field. This article reviews the current applications of F.D.G.-PET in patients with cervix cancer. (authors)

  10. Dosimetric comparison of IMRT and modulated arc-therapy techniques in the treatment of cervical cancers; Comparaison dosimetrique des techniques de RCMI et d'arctherapie modulee dans le traitement des cancers du col uterin

    Energy Technology Data Exchange (ETDEWEB)

    Renard-Oldrini, S.; Charra-Brunaud, C.; Tournier-Rangeard, L.; Huger, S.; Marchesi, V.; Bouziz, D.; Peiffert, D. [Centre Alexis-Vautrin, Nancy (France)

    2011-10-15

    The authors report the dosimetric comparison of two techniques used for the treatment of cervical cancers: the intensity-modulated conformational radiotherapy (IMRT) with static beams and modulated arc-therapy with RapidArc. The treatment plans of 15 patients have been compared. The clinical target volume (CTV) comprises the gross target volume, the cervix, the upper third of the vagina, and ganglionary areas. The previsional target volume comprises the clinical target volume and a one centimetre margin. Organs at risk are rectum, bladder, intestine and bone marrow. Arc-therapy seems to provide a better sparing of intestine that IMRT, while maintaining a good coverage of the previsional target volume and decreasing treatment duration. Short communication

  11. Current data on radio chemotherapy and potential of targeted therapies for cervical cancers; Donnees actuelles des associations chimioradiotherapeutiques et place potentielle des therapies ciblees dans les cancers du col uterin

    Energy Technology Data Exchange (ETDEWEB)

    Magne, N.; Deutsch, E.; Haie-Meder, C. [Institut Gustave-Roussy, Unite de Curietherapie, Dept. de Radiotherapie, 94 - Villejuif (France)

    2008-01-15

    The present review represents an up-to-date focus on the particular topic of cervix carcinoma. An exhaustive description of the actual data and the near-future combination of radiotherapy and drugs with the specific potential of targeted therapies are presented. This approach represents one of the next challenges to improve results. Studies conducted in 1999, 2000, and 2002 reported the results of six large-scale prospective randomized trials using concomitant chemo radiation with a significant progression-free and overall survival rate improvement compared to radiotherapy only. These results were confirmed by the two last meta-analyses. Nowadays, the concurrent radio chemotherapy schedule used in the treatment of high risk cervical cancer is a standard practice. More growing evidences suggest that intracellular signal pathways play a significant role in radiation response. Several prognostic factors on tumoral radiosensitivity have been identified, including intracellular signal pathways, in the particular case of cervix carcinoma. Promising results have been obtained in experimental studies assessing the combined use of specific inhibitors and radiotherapy. Based on these data, a number of clinical trials have been started to enhance tumor responses and thus, to decrease the rate of recurrences. (authors)

  12. Structural changes in mixed Col I/Col V collagen gels probed by SHG microscopy: implications for probing stromal alterations in human breast cancer.

    Science.gov (United States)

    Ajeti, Visar; Nadiarnykh, Oleg; Ponik, Suzanne M; Keely, Patricia J; Eliceiri, Kevin W; Campagnola, Paul J

    2011-08-01

    Second Harmonic Generation (SHG) microscopy has been previously used to describe the morphology of collagen in the extracellular matrix (ECM) in different stages of invasion in breast cancer. Here this concept is extended by using SHG to provide quantitative discrimination of self-assembled collagen gels, consisting of mixtures of type I (Col I) and type V (Col V) isoforms which serve as models of changes in the ECM during invasion in vivo. To investigate if SHG is sensitive to changes due to Col V incorporation into Col I fibrils, gels were prepared with 0-20% Col V with the balance consisting of Col I. Using the metrics of SHG intensity, fiber length, emission directionality, and depth-dependent intensities, we found similar responses for gels comprised of 100% Col I, and 95% Col I/5% Col V, where these metrics were all significantly different from those of the 80% Col I/20% Col V gels. Specifically, the gels of lower Col V content produce brighter SHG, are characterized by longer fibers, and have a higher forward/backward emission ratio. These attributes are all consistent with more highly organized collagen fibrils/fibers and are in agreement with previous TEM characterization as well as predictions based on phase matching considerations. These results suggest that SHG can be developed to discriminate Col I/Col V composition in tissues to characterize and follow breast cancer invasion.

  13. Facteurs liés au diagnostic tardif du cancer du sein: expérience du CHU Mohammed VI Marrakech

    OpenAIRE

    Aloulou, Sofia; El Mahfoudi, Amal; El Omrani, Abdelhamid; Khouchani, Mouna

    2015-01-01

    Le cancer du sein est le premier cancer féminin en termes d'incidence et de mortalité. Au Maroc, il vient au premier rang des cancers de la femme avant celui du col utérin Il constitue un problème de santé publique. Son pronostic est étroitement lié au stade auquel le diagnostic est posé. Il s'agit d'une pathologie dont les moyens diagnostiques sont de nos jours développés, allant de la détection précoce à la mise en évidence de lésions infra-cliniques, ce qui a nettement amélioré le pronosti...

  14. Differentiation of Col I and Col III isoforms in stromal models of ovarian cancer by analysis of second harmonic generation polarization and emission directionality.

    Science.gov (United States)

    Tilbury, Karissa; Lien, Chi-Hsiang; Chen, Shean-Jen; Campagnola, Paul J

    2014-01-21

    A profound remodeling of the extracellular matrix occurs in many epithelial cancers. In ovarian cancer, the minor collagen isoform of Col III becomes upregulated in invasive disease. Here we use second harmonic generation (SHG) imaging microscopy to probe structural differences in fibrillar models of the ovarian stroma comprised of mixtures of Col I and III. The SHG intensity and forward-backward ratios decrease with increasing Col III content, consistent with decreased phasematching due to more randomized structures. We further probe the net collagen α-helix pitch angle within the gel mixtures using what is believed to be a new pixel-based polarization-resolved approach that combines and extends previous analyses. The extracted pitch angles are consistent with those of peptide models and the method has sufficient sensitivity to differentiate Col I from the Col I/Col III mixtures. We further developed the pixel-based approach to extract the SHG signal polarization anisotropy from the same polarization-resolved image matrix. Using this approach, we found that increased Col III results in decreased alignment of the dipole moments within the focal volume. Collectively, the SHG measurements and analysis all indicate that incorporation of Col III results in decreased organization across several levels of collagen organization. Furthermore, the findings suggest that the collagen isoforms comingle within the same fibrils, in good agreement with ultrastructural data. The pixel-based polarization analyses (both excitation and emission) afford determination of structural properties without the previous requirement of having well-aligned fibers, and the approaches should be generally applicable in tissue.

  15. Métastases gastro-intestinales du cancer du sein: à propos de 2 cas

    Science.gov (United States)

    Loubna, Mezouar; Mohamed, El Hfid; Tijani, El Harroudi; Fouzia, Ghadouani; Hanane, Haj Kacem; Zouhour, Bourhaleb; Asmae, Ouabdelmoumen

    2013-01-01

    Le cancer du sein est le cancer le plus fréquent chez la femme, notamment au Maroc, avec un taux de mortalité élevé. Les métastases gastro-intestinales d'un carcinome canalaire du sein sont rares. Leur diagnostic est difficile du fait de la nature non spécifique des symptômes. Nous rapportons deux observations originales de métastases gastroduodénales d'un cancer canalaire infiltrant du sein. Les métastases gastro-intestinales du cancer du sein sont très rares; la présence de symptômes gastro-intestinaux chez une malade ayant un antécédent de cancer du sein doit faire suspecter une atteinte métastatique gastro-intestinale. PMID:24198876

  16. Wavelength-Dependent Second Harmonic Generation Circular Dichroism for Differentiation of Col I and Col III Isoforms in Stromal Models of Ovarian Cancer Based on Intrinsic Chirality Differences.

    Science.gov (United States)

    Campbell, Kirby R; Campagnola, Paul J

    2017-03-02

    Extensive remodeling of the extracellular matrix (ECM) occurs in many epithelial cancers. For example, in ovarian cancer, upregulation of collagen isoform type III has been linked to invasive forms of the disease, and this change may be a potential biomarker. To examine this possibility, we implemented wavelength-dependent second harmonic generation circular dichroism (SHG-CD) imaging microscopy to quantitatively determine changes in chirality in ECM models comprised of different Col I/Col III composition. In these models, Col III was varied between 0 and 40%, and we found increasing Col III results in reduced net chirality, consistent with structural biology studies of Col I and III in tissues where the isoforms comingle in the same fibrils. We further examined the wavelength dependence of the SHG-CD to both optimize the response and gain insight into the underlying mechanism. We found using shorter SHG excitation wavelengths resulted in increased SHG-CD sensitivity, where this is consistent with the electric-dipole-coupled oscillator model suggested previously for the nonlinear chirality response from thin films. Moreover, the sensitivity is further consistent with the wavelength dependency of SHG intensity fit to a two-state model of the two-photon absorption in collagen. We also provide experimental calibration protocols to implement the SHG-CD modality on a laser scanning microscope. We last suggest that the technique has broad applicability in probing a wide range of diseased states with changes in collagen molecular structure.

  17. Modeling near-surface firn temperature in a cold accumulation zone (Col du Dôme, French Alps): from a physical to a semi-parameterized approach

    Science.gov (United States)

    Gilbert, A.; Vincent, C.; Six, D.; Wagnon, P.; Piard, L.; Ginot, P.

    2014-04-01

    Analysis of the thermal regime of glaciers is crucial for glacier hazard assessment, especially in the context of a changing climate. In particular, the transient thermal regime of cold accumulation zones needs to be modeled. A modeling approach has therefore been developed to determine this thermal regime using only near-surface boundary conditions coming from meteorological observations. In the first step, a surface energy balance (SEB) model accounting for water percolation and radiation penetration in firn was applied to identify the main processes that control the subsurface temperatures in cold firn. Results agree well with subsurface temperatures measured at Col du Dôme (4250 m above sea level (a.s.l.)), France. In the second step, a simplified model using only daily mean air temperature and potential solar radiation was developed. This model properly simulates the spatial variability of surface melting and subsurface firn temperatures and was used to accurately reconstruct the deep borehole temperature profiles measured at Col du Dôme. Results show that percolation and refreezing are efficient processes for the transfer of energy from the surface to underlying layers. However, they are not responsible for any higher energy uptake at the surface, which is exclusively triggered by increasing energy flux from the atmosphere due to SEB changes when surface temperatures reach 0 °C. The resulting enhanced energy uptake makes cold accumulation zones very vulnerable to air temperature rise.

  18. Modeling near-surface firn temperature in a cold accumulation zone (Col du Dôme, French Alps: from a physical to a semi-parameterized approach

    Directory of Open Access Journals (Sweden)

    A. Gilbert

    2013-11-01

    Full Text Available Analysis of the thermal regime of glaciers is crucial for glacier hazard assessment, especially in the context of a changing climate. In particular, the transient thermal regime of cold accumulation zones needs to be modeled. A modeling approach has therefore been developed to determine this thermal regime using only near-surface boundary conditions coming from meteorological observations. In the first step, a surface energy-balance (SEB model accounting for water percolation was applied to identify the main processes that control the subsurface temperatures in cold firn. Results agree well with subsurface temperatures measured at Col du Dôme (4250 m a.s.l., France. In the second step, a simplified model using only daily mean air temperature and potential solar radiation was developed. This model properly simulates the spatial variability of surface melting and subsurface firn temperatures and was used to accurately reconstruct the deep borehole temperature profiles measured at Col du Dôme. Results show that percolation and refreezing are efficient processes for the transfer of energy from the surface to underlying layers. However, they are not responsible for any higher energy uptake at the surface, which is exclusively triggered by increasing energy flux from the atmosphere due to SEB changes when surface temperature reach 0 °C. The resulting enhanced energy uptake makes cold accumulation zones very vulnerable to air temperature rise.

  19. Oncoplastie avec conservation mammaire dans le traitement du cancer du sein: à propos de 16 cas

    OpenAIRE

    2015-01-01

    Le cancer du sein est actuellement le cancer le plus fréquent chez la femme, et pose un véritable problème diagnostique et thérapeutique. Le dépistage des lésions à un stade de plus en plus précoce, a permis une extension des indications du traitement conservateur radiochirurgical, qui était initialement limitées aux tumeurs de moins de 3 cm, unifocales, non inflammatoires. Par ailleurs, l'utilisation de traitements préopératoires permet d’étendre les indications du traitement conservateur à ...

  20. Anal cancer; Cancer du canal anal

    Energy Technology Data Exchange (ETDEWEB)

    Fesneau, M.; Champeaux-Orange, E. [Service de radiotherapie, Centre regional universitaire de cancerologie Henry-S.-Kaplan CHU de Tours, Hopital Bretonneau, 37 - Tours (France); Champeaux-Orange, E. [Service d' oncologie-radiotherapie, Centre hospitalier regional d' Orleans, 45 - Orleans (France); Hennequin, C. [Service de cancerologie-radiotherapie, hopital Saint-Louis, 75 - Paris (France)

    2010-07-01

    Anal canal epidermoid carcinomas represent 1.2% of digestive cancers and 6% of ano-rectal cancers. For localized diseases, the treatment is based on radiotherapy with or without chemotherapy (5-FU and cisplatin or mitomycin), according to tumour and nodal extension. The recommended treatment dose is 45 Gy in the anal canal, the mesorectum, para-rectal lymph nodes, and inguinal lymph nodes. An additional dose of 15 to 20 Gy is delivered in the initial tumour for good responders. Salvage surgery is necessary in case of poor response. The organs at risk to be considered are bladder, femur heads, small intestine and vulva. The objective of this work is to summarize the epidemiological and radio-anatomic and prognostic characteristics of this tumour. The conformal radiotherapy technique is illustrated by a case report. (authors)

  1. Coléoptères du Szé-tchouen et de Koui-tchéou (Chine)

    NARCIS (Netherlands)

    Fairmaire, L.

    1897-01-01

    Deux individus du Szé-tchouen se rapportent à la description et à la figure de Redtenbacher. Mais chez l’un les expansions du corselet sont plus développés et l’angle latéro-postérieur dépasse fortement les bords latéraux en formant presque un crochet très pointu. La tête n’est pas aussi triangulair

  2. Three-dimensional brachytherapy optimization techniques in the treatment of patients with cervix cancer; Apport des techniques de curietherapie optimisee grace a l'imagerie tridimensionnelle dans la prise en charge des patientes atteintes d'un cancer du col uterin

    Energy Technology Data Exchange (ETDEWEB)

    Haie-Meder, C.; Mazeron, R.; Verezesan, O.; Monnier, L.; Vieillot, S. [Institut Gustave-Roussy, Service de Curietherapie, 94 - Villejuif (France); Dumas, I. [Institut Gustave-Roussy, Service de Physique, 94 - Villejuif (France); Lhomme, C. [Institut Gustave-Roussy, Service d' Ooncologie Gynecologique, 94 - Villejuif (France); Morice, P. [Institut Gustave-Roussy, Service de Chirurgie Oncologique, 94 - Villejuif (France); Barillot, I. [Centre Regional Universitaire de Cancerologie Henry-S.-Kaplan, Hopital Bretonneau, CHU de Tours, 37 - Tours (France); Universite Francois-Rabelais, 37 - Tours (France)

    2009-10-15

    Traditionally, prescription and treatment planning in intracavitary brachytherapy for cervix cancer have used either reference points (mainly points A and B) or reference isodoses (60 Gy according to ICRU recommendations) to report doses to the target volume. Doses to critical organs were reported at bladder and rectum ICRU points. This practice has been supported by a long-standing clinical experience that has yielded an acceptable therapeutic ratio. The recent development of imaging has contributed to the improvement in target and organs at risk knowledge. In 2005 and 2006, the European group of brachytherapy -European Society for therapeutic radiology and oncology (GEC-E.S.T.R.O.) recommendations publications on 3-D based image brachytherapy have defined the different volumes of interest. These recommendations have been validated with intercomparison delineation studies. With the concomitant development of remote after-loading projectors, provided with miniaturized sources, it is now possible to plan radiation doses by adjusting dwell positions and relative dwell time values. These procedures allow better coverage of the targets while sparing O.A.R.. The recent literature data evidence a significant improvement in local control with no increase in complications. Further studies are needed to better define the dose recommended in both tumour and organs at risk. This is one of the goals of the European study on MRI-guided brachytherapy in locally advanced cervical cancer (E.M.B.R.A.C.E.) protocol (meaning of acronym: an international study on MRI-guided brachytherapy in locally advanced cervical cancer). (authors)

  3. Contrast ultrasound using SonoVue for pelvic radiation with concurrent chemotherapy monitoring in stage I B-II cervical cancer; Apport de l'echographie Doppler avec injection de contraste pour la prise en charge des cancers du col de l'uterus de stades IB et II par chimioradiotherapie concomitante

    Energy Technology Data Exchange (ETDEWEB)

    Marret, H. [Hopital Bretonneau, Service de Gynecologie, 37 - Tours (France); Barillot, I. [Hopital Bretonneau, Service de Radiotherapie, 37 - Tours (France); Rolland, Y. [CRLCC Eugene-Marquis, 35 - Rennes (France); Leveque, J. [Hopital Anne-de-Bretagne, 35 - Rennes (France)

    2009-10-15

    The aim of the present study was to evaluate whether angiogenic parameters as assessed by trans vaginal color Doppler ultrasound and by enhanced cervix ultrasound may predict prognostic factors of stage I B greater than 4 cm and II cervical cancer treated by radio chemotherapy. A total of 40 patients with histologically proven advanced stage invasive cervical cancer will be evaluated by color Doppler, contrast ultrasound, and MRI before radiotherapy, after the second chemotherapy cycle and prior to surgery. Subjective assessment of the amount of vessels within the tumor (scanty-moderate or abundant) and resistance index (R.I.) will be recorded for Doppler, enhancement and washout period will be studied after injection of SonoVue for contrast ultrasound. All patients will have radical hysterectomy and pelvic lymph node dissection. Comparison with MRI results will be done. The main parameter studied will be persistence of tumor cells in the analysis of the radical hysterectomy. We expected to correlate contrast parameters with persistent disease in order to confirm ultrasound parameters that will be useful to monitor radio chemotherapy and to predict therapeutic answer of such treatment. (authors)

  4. Description de quatre espèces nouvelles du genre Paropsis, Oliv. (Coléoptères Phytophages)

    NARCIS (Netherlands)

    Duvivier, Ant.

    1884-01-01

    Corps subhémisphérique, très-convexe; dessous d’un brun de poix presque noir au milieu; dessus d’un noir brillant, excepté le labre, le vertex, les côtés et le bord antérieur du prothorax, et deux grandes taches sur chaque élytre qui sont rouges ou testacés; ponctué sur toute la surface, les élytres

  5. Concomitant pelvic irradiation and chemotherapy in locally advanced cervical carcinoma. A retrospective study of 92 patients treated at the Curie Institute; Chimioradiotherapie dans les cancers du col uterin localement evolues. Etude retrospective de 92 patientes traitees a l'Institut Curie de 1986 a 1998

    Energy Technology Data Exchange (ETDEWEB)

    Nguyen, D.; Rochefordiere, A. de la; Chauveinc, L.; Cosset, J.M. [Institut Curie, Dept. de Radiotherapie, 75 - Paris (France); Clough, K.B. [Institut Curie, Dept. de Chirurgie, 75 - Paris (France); Mouret-Fourme, E.; Guyonnet, M. [Institut Curie, Service de Biostatistiques, 75 - Paris (France)

    2002-06-01

    The prognosis of locally advanced cervix cancers is poor with metastatic and local recurrence risks. Recent publications reported that concurrent chemotherapy and pelvic radiation increased local control compared to radiotherapy alone. Chemotherapy could also decrease metastatic recurrences. We report 92 cases of patients with locally advanced cervix cancer treated between 1986 and 1998 at the Institut Curie. Patients and methods. - Concurrent chemo-radiation was exclusive in 51 cases and added to surgery in 41 cases. Chemotherapy with 5FU -Cisplatin-Mitomycin C-Vindesin (protocol A) was performed for 43% of patients and 57% of them received 5FU-Cisplatin alone (protocol B). Results. -Median follow-up was 64 months (6-149 months). Five-year disease-free survival rate was 47% and local control rate was 70%. Disease-free survival was correlated with therapeutic response. After exclusive chemo-radiation, the good responsive patients had a better DFS (54% vs 26%, p=0.018). In the surgery group, those patients with sterilized lymph nodes and tumours had also a higher DFS (76% vs 47%, p=0.036). Toxicity was higher with protocol A. Conclusion. - From our study, it appears that local control of advanced cervix cancers is better with combined chemoradiotherapy but disease-free survival stays low according to the metastatic evolution. Metastasis without local recurrence remained frequent in our study. 5FU-CDDP chemotherapy has a lower toxicity and is as effective as 5FU-CDDP-Mitomycin C-Vindesin protocol, in association with radiotherapy. (author)

  6. Classification moléculaire du cancer du sein au Maroc

    OpenAIRE

    Fouad, Abbass; Yousra, Akasbi; Kaoutar, Znati; Omar, El Mesbahi; Afaf, Amarti; Sanae, Bennis

    2012-01-01

    Introduction La classification moléculaire des cancers du sein basée sur l'expression génique puis sur le profil protéique a permis de distinguer cinq groupes moléculaires: luminal A, luminal B, Her2/neu, basal-like et non-classées. L'objectif de cette étude réalisée au CHU Hassan II de Fès est de classer 335 cancers du sein infiltrant en groupes moléculaires, puis de les corréler avec les caractéristiques clinicopathologiques. Méthodes Etude rétrospective étalée sur 45 mois, comportant 335 p...

  7. Oncoplastie avec conservation mammaire dans le traitement du cancer du sein: à propos de 16 cas

    Science.gov (United States)

    Bouzoubaa, Wail; Laadioui, Meryam; Jayi, Sofia; Alaoui, Fatime Zahra Fdili; Bouguern, Hakima; Chaara, Hikmat; Melhouf, Moulay Abdelilah

    2015-01-01

    Le cancer du sein est actuellement le cancer le plus fréquent chez la femme, et pose un véritable problème diagnostique et thérapeutique. Le dépistage des lésions à un stade de plus en plus précoce, a permis une extension des indications du traitement conservateur radiochirurgical, qui était initialement limitées aux tumeurs de moins de 3 cm, unifocales, non inflammatoires. Par ailleurs, l'utilisation de traitements préopératoires permet d’étendre les indications du traitement conservateur à des tumeurs plus volumineuses. Parallèlement à cette extension des indications de conservation mammaire, on a observé le développement de nouvelles approches thérapeutiques notamment la chirurgie oncoplastique, technique du ganglion sentinelle et chirurgie stéréotaxique, dont les résultats initiaux sont très encouragent. A travers cette étude réalisée dans le service de gynécologie et obstétrique II du CHU HASSAN II de FES au MAROC, après l'analyse rétrospective de 16 patientes traitées par traitement conservateur et oncoplastie, nous avons voulus montrer notre aptitude a réalisé ses techniques chirurgicales et a bien prendre en charge ces patientes, mais aussi évaluer ces techniques en termes de résultat carcinologique et de résultat esthétique, aussi en terme de survie globale, survie sans métastase et en termes de récidive locale entre les plasties mammaires et les traitements usuels: mastectomie et traitement conservateur classique. PMID:26430477

  8. Evaluation épidémiologique du programme genevois de dépistage du cancer du sein : 2007-2011

    OpenAIRE

    2014-01-01

    A l'instar de nombreux pays industrialisés, le cancer du sein est à Genève le cancer le plus fréquent (environ 460 cas par an) et la première cause de décès chez les femmes entre 45 et 55 ans. Depuis mars 1999, le Programme genevois de dépistage du cancer du sein a pour missions de promouvoir, d'organiser et de mener une action de prévention auprès de la population féminine du canton âgée de 50 à 69 ans. Ce rapport décrit l'évolution de 15 ans d'activité de dépistage (chapitre 2) et analys...

  9. L'invisibilité du cancer en Afrique

    NARCIS (Netherlands)

    F. Chabrol; V.K. Nguyen

    2013-01-01

    L’Afrique australe se remet à peine de l’épidémie de sida, grâce à une mobilisation transnationale sans précédent et à des financements massifs pour acheminer les antirétroviraux, qu’une épidémie de cancer prolifère dans le sillon du VIH. Comment rendre compte de ce drame oublié des politiques de sa

  10. Classification moléculaire du cancer du sein au Maroc

    Science.gov (United States)

    Fouad, Abbass; Yousra, Akasbi; Kaoutar, Znati; Omar, El Mesbahi; Afaf, Amarti; Sanae, Bennis

    2012-01-01

    Introduction La classification moléculaire des cancers du sein basée sur l'expression génique puis sur le profil protéique a permis de distinguer cinq groupes moléculaires: luminal A, luminal B, Her2/neu, basal-like et non-classées. L'objectif de cette étude réalisée au CHU Hassan II de Fès est de classer 335 cancers du sein infiltrant en groupes moléculaires, puis de les corréler avec les caractéristiques clinicopathologiques. Méthodes Etude rétrospective étalée sur 45 mois, comportant 335 patientes colligées au CHU pour le diagnostic et le suivi. Les tumeurs sont analysées histologiquement et classées après une étude immunohistochimique en groupes: luminal A, luminal B, Her2+, basal-like et non-classées. Résultats 54.3% des tumeurs sont du groupe luminal A, 16% luminal B, 11.3% Her2+, 11.3% basal-like et 7% non-classées. Le groupe luminal A renferme le plus faible taux de grade III, d'emboles vasculaires ainsi que de métastases; alors que le groupe des non-classées et basal-like représentent un taux élevé de grade III, une faible proportion d'emboles vasculaires et d'envahissement ganglionnaire. Ces facteurs sont significativement élevés dans les groupes luminal B et Her2+ avec un taux de survie globale de 78% et 76% respectivement. Dans le groupe luminal A, la survie globale des patientes est élevée (87%) alors qu'elle n'est que de 49% dans le groupe des triples négatifs (basal-like et non-classés). Conclusion Le groupe luminal B est différent du luminal A et il est de pronostic péjoratif vis à vis du groupe Her2+. Les caractéristiques clinicopathologiques concordent avec le profil moléculaire donc devraient être pris en considération comme facteurs pronostiques. PMID:23396646

  11. Radon and lung cancer; Radon et cancer du poumon

    Energy Technology Data Exchange (ETDEWEB)

    Baysson, H; Billon, S.; Catelinois, O.; Gambard, J.P.; Laurier, D.; Rogel, A.; Tirmarche, M

    2004-12-01

    Radon is a natural radioactive gas that tends to accumulate in indoor environments; its concentration is highest in areas with granite sub-soils. Epidemiologic studies of uranium miners and animal data demonstrate the radon inhalation increases the risk of lung cancer. The objective of this paper is to present the available data on the French population's exposure to radon and the current epidemiologic knowledge of its effects, from cohort studies of uranium miners and indoor radon case-control studies.

  12. Les déterminants du statut “perdu de vue” chez les patients pris en charge pour cancer au Maroc: situation avant le Plan Cancer

    Science.gov (United States)

    Najdi, Adil; Berraho, Mohamed; Bendahhou, Karima; Obtel, Majdouline; Zidouh, Ahmed; Errihani, Hassan; Nejjari, Chakib

    2014-01-01

    Introduction Le cancer au Maroc représente un problème majeur de santé publique, sa prise en charge doit être globale, active et complète pour tous les patients. L'objectif de ce travail était d'estimer la fréquence des perdus de vue « PDV » en oncologie au Maroc durant la première année de suivi et de déterminer les facteurs associés à ce problème. Méthodes Par une étude rétrospective portant sur 2854 dossiers de malades hospitalisés dans les trois principaux centres d'oncologie au Maroc depuis janvier 2003 jusqu’à juin 2007 et concernant les cinq principales localisations de cancer au Maroc, nous avons cherché la date des dernières nouvelles des patients ayant un recul de 18 mois minimum afin de déterminer le statut de ces malades après un an de suivi. Résultats La moyenne d’âge était de 52±14 ans, une proportion féminine de 63%, les sujets actifs constituaient 28%, les mariés 71%, les analphabètes 51%, 70% des patients habitaient en milieu urbain et seulement 11% des malades disposaient d'une couverture sociale. La localisation cancéreuse la plus fréquente était le poumon (23,8%) suivie du colon-rectum (23,5%) puis le col (21,9%), le sein (20,4%) et les lymphomes (10,4%). Le taux des «PDV» à un an de suivi était de 48%, ce statut était significativement lié au sexe, à l’âge, au NSE et au statut matrimonial. Sur le plan médical, le statut «PDV» était lié à la localisation du cancer, au stade de diagnostic et au type de traitement reçu. Conclusion Notre étude a mis en évidence la grande ampleur du problème des PDV en cancérologie au Maroc ainsi que ces déterminants. Ces résultats incitent tous les acteurs dans le domaine de la cancérologie à collaborer ensemble pour prendre les mesures qui s'imposent pour y pallier PMID:25400850

  13. Conservative treatment for breast cancer: optimization of the tumor bed localization; Traitement conservateur du cancer du sein: optimisation du reperage du lit tumoral

    Energy Technology Data Exchange (ETDEWEB)

    Oden, S.; Thureau, S.; Hanzen, C. [Centre Henri-Becquerel, Dept. de Radiotherapie et de Physique Medicale, 76 - Rouen (France); Oden, S. [CHU de Rouen, Service de Gynecologie et Obstetrique, 76 - Rouen (France); Baron, M. [Centre Henri-Becquerel, Dept. de Chirurgie, 76 - Rouen (France)

    2010-04-15

    Purpose The main objective was to assess contributions and limits of surgical clips combined with computed tomography to define the tumor bed after conservative treatment of breast cancer. This retrospective observational study enrolled 16 patients treated by lumpectomy with surgical clips placed in the tumor bed. Patients and methods We assessed the difficulties in localizing the tumour bed based on collected data (i.e. clinical description, mammography, ultrasound examination, surgical procedure and pathology data). The clip's number and localization, and the volumes of the boost were also analyzed. Results There was no preoperative occurrence of localization in 57 % of cases for palpable tumours, and in 13 % of cases where the lesion was subclinical. The collected data did not allow establishing a precise localization. The mean number of surgical clips per patient was 4 (range 1-9), and the delineation of the target volumes by radiation oncologists was not standardized. Onco plastic techniques may produce difficulties in the localization of tumour bed. Conclusion The placement of surgical clips by the surgeon is helpful, primarily when onco plastic techniques are used. In many situations, these clips may cause problems of interpretation. In order to optimize the delineation of the boost, we propose a multidisciplinary approach and methodology to be used at the Henri Becquerel Cancer Centre. (authors)

  14. Learning about Cri du Chat Syndrome

    Science.gov (United States)

    ... Learning About Prostate Cancer Learning About Cri du Chat Syndrome What is cri du chat syndrome? What ... cri du chat syndrome What is cri du chat syndrome? Cri du chat syndrome - also known as ...

  15. [The Bulletin du cancer: the years before the First World War].

    Science.gov (United States)

    Bernaudin, Jean-François

    2013-12-01

    Three years after its founding in 1909, the Association française pour l'étude du cancer is a major scientific society developing transdisciplinary debates particularly on innovative therapeutics in cancer, such as the developing use of radium. The Association at that time assembles together all the French medical elite. Reading the Bulletin offers a clear view of the brilliant monthly debates. First World War stopped the life of the Association for four years. After this break, the set up of dedicated centers for cancer treatment was responsible for a major turn in the Association's life.

  16. Aspects cliniques des cancers bronchopulmonaires primitifs au service d'oncologie du CHUA-HUJRA Antananarivo

    OpenAIRE

    Refeno, Valéry; Hasiniatsy, Nomeharisoa Rodrigue Emile; Andrianandrasana, Ny Ony Tiana Florence; Ramahandrisoa, Andriatsihoarana Voahary Nasandratriniavo; Rakotonarivo, Jean Marc; Maevazaka, Joée Larissa; Rakotovao, Hanitrala Jean Louis; Rafaramino, Florine

    2015-01-01

    Le retard de diagnostic des cancers broncho-pulmonaires est l'une des sources du retard de leur prise en charge dans les pays en développement. A notre connaissance, l'aspect clinique des cancers broncho-pulmonaires au Centre Hospitalier Universitaire d'Antananarivo-Hôpital Universitaire Joseph Ravoahangy Andrianavalona (CHUA-HUJRA) n'a jamais été étudié. L'objectif était de décrire les aspects cliniques des cancers broncho-pulmonaires primitifs dans le plus grand centre de cancérologie de Ma...

  17. Anthocyanin Induces Apoptosis of DU-145 Cells In Vitro and Inhibits Xenograft Growth of Prostate Cancer

    Science.gov (United States)

    Ha, U-Syn; Bae, Woong Jin; Kim, Su Jin; Yoon, Byung Il; Hong, Sung Hoo; Lee, Ji Youl; Hwang, Tae-Kon; Hwang, Sung Yeoun; Wang, Zhiping

    2015-01-01

    Purpose To investigate the effects of anthocyanins extracted from black soybean, which have antioxidant activity, on apoptosis in vitro (in hormone refractory prostate cancer cells) and on tumor growth in vivo (in athymic nude mouse xenograft model). Materials and Methods The growth and viability of DU-145 cells treated with anthocyanins were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and apoptosis was assessed by DNA laddering. Immunoblotting was conducted to evaluate differences in the expressions of p53, Bax, Bcl, androgen receptor (AR), and prostate specific antigen (PSA). To study the inhibitory effects of anthocyanins on tumor growth in vivo, DU-145 tumor xenografts were established in athymic nude mice. The anthocyanin group was treated with daily oral anthocyanin (8 mg/kg) for 14 weeks. After 2 weeks of treatment, DU-145 cells (2×106) were inoculated subcutaneously into the right flank to establish tumor xenografts. Tumor dimensions were measured twice a week using calipers and volumes were calculated. Results Anthocyanin treatment of DU-145 cells resulted in 1) significant increase in apoptosis in a dose-dependent manner, 2) significant decrease in p53 and Bcl-2 expressions (with increased Bax expression), and 3) significant decrease in PSA and AR expressions. In the xenograft model, anthocyanin treatment significantly inhibit tumor growth. Conclusion This study suggests that anthocyanins from black soybean inhibit the progression of prostate cancer in vitro and in a xenograft model. PMID:25510742

  18. Facteurs de risque de cancer du poumon chez la femme

    OpenAIRE

    Papadopoulos, Alexandra

    2012-01-01

    The incidence of female lung cancer in developed countries has been increasing since 1950 and particularly in France where the cigarettes consumption has also increased. Since 1980, a growing number of epidemiological surveys have pinpointed the risk of female lung cancer related to smoking. Consecutively, a debate on gender differences in lung cancer risk has appeared, but still in progress nowadays. The reproductive factors could explain these differences. In order to have recent and reliab...

  19. Health survey on cancers about the Tricastin nuclear site; Etude sanitaire sur les cancers autour du site nucleaire du Tricastin

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2010-07-01

    This survey aims at describing the health status of the population around the Tricastin site, and more particularly at determining whether there is a difference between death or cancer occurrence frequencies observed around this site with respect to reference frequencies. It does not aim at assessing the health impact of the site industrial installations. Cancer mortality data, cancer diagnosis data, demographic data, child cancer data, data related to hospital stays in relationship with cancer, long duration hospital stay data, and mortality data are used. Several indicators are defined and used: standardised mortality ratio, standardised hospitalisation ratio. Data are also analysed in terms of location, and socio-demographic categories. It appears that there is no specific health situation for the considered area, except for pancreas cancer for women

  20. The use of matrigel has no influence on tumor development or PET imaging in FaDu human head and neck cancer xenografts

    DEFF Research Database (Denmark)

    Fliedner, Frederikke P.; Hansen, Anders Elias; Jorgensen, Jesper T.

    2016-01-01

    is currently available. This study evaluates the potential effect of matrigel use in a human head and neck cancer xenograft model (FaDu; hypopharyngeal carcinoma) in NMRI nude mice. The FaDu cell line was chosen based on its frequent use in studies of cancer imaging and tumor microenvironment. Methods: NMRI...

  1. Environmentamb-Col

    OpenAIRE

    2010-01-01

    Environmentamb-col es una organización privada para el tratamiento de residuos sólidos de la industria de Cundinamarca. Su propósito es mejorar la calidad del ambiente y desarrollar conciencia ecológica en la población urbana y rural. Ubicada en la cabecera del municipio de Chocontá, la organización cuenta con una planta de tratamiento de residuos, donde se recicla y separa el material desechado

  2. Identification et prise en charge des femmes ayant des antécédents familiaux de cancer du sein

    Science.gov (United States)

    Heisey, Ruth; Carroll, June C.

    2016-01-01

    Résumé Objectif Résumer les meilleures données portant sur les stratégies d’identification et de prise en charge des femmes qui présentent des antécédents familiaux de cancer du sein. Sources d’information Une recherche a été effectuée sur PubMed à l’aide des mots-clés anglais suivants : breast cancer, guidelines, risk, family history, management et magnetic resonance imaging screening, entre 2000 et 2016. La plupart des données sont de niveau II. Message principal Une bonne anamnèse familiale est essentielle lors de l’évaluation du risque de cancer du sein afin d’identifier les femmes qui sont candidates à une recommandation en counseling génétique pour un éventuel test génétique. On peut sauver des vies en offrant aux femmes porteuses d’une mutation au gène BRCA des interventions chirurgicales de réduction des risques (mastectomie bilatérale prophylactique, salpingo-ovariectomie bilatérale). Il faut encourager toutes les femmes qui présentent des antécédents familiaux de cancer du sein à demeurer actives et à limiter leur consommation d’alcool à moins de 1 verre par jour; certaines femmes sont admissibles à la chimioprévention. Il faut offrir aux femmes dont le risque à vie de cancer du sein est de 20 à 25 % ou plus un dépistage poussé par imagerie par résonance magnétique en plus d’une mammographie. Conclusion Une vie saine et la chimioprévention (chez les candidates) pourraient réduire l’incidence du cancer du sein; le dépistage poussé pourrait entraîner une détection plus précoce. Le fait d’aiguiller des femmes porteuses d’une mutation au BRCA vers la chirurgie de réduction des risques sauve des vies. PMID:27737991

  3. Role of IGF-1/IGF-1R in regulation of invasion in DU145 prostate cancer cells

    Science.gov (United States)

    Saikali, Zeina; Setya, Hemani; Singh, Gurmit; Persad, Sujata

    2008-01-01

    Background Prostate cancer progression to androgen independence is the primary cause of mortality by this tumor type. The IGF-1/IGF-1R axis is well known to contribute to prostate cancer initiation, but its contribution to invasiveness and the downstream signalling mechanisms that are involved are unclear at present. Results We examined the invasive response of androgen independent DU145 prostate carcinoma cells to IGF-1 stimulation using Matrigel assays. We then examined the signaling mechanisms and protease activities that are associated with this response. IGF-1 significantly increased the invasive capacity of DU145 cells in vitro, and this increase was inhibited by blocking IGF-1R. We further demonstrated that specific inhibitors of the MAPK and PI3-K pathways decrease IGF-1-mediated invasion. To determine potential molecular mechanisms for this change in invasive capacity, we examined changes in expression and activity of matrix metalloproteinases. We observed that IGF-1 increases the enzymatic activity of MMP-2 and MMP-9 in DU145 cells. These changes in activity are due to differences in expression in the case of MMP-9 but not in the case of MMP-2. This observation is corroborated by the fact that correlated changes of expression in a regulator of MMP-2, TIMP-2, were also seen. Conclusion This work identifies a specific effect of IGF-1 on the invasive capacity of DU145 prostate cancer cells, and furthermore delineates mechanisms that contribute to this effect. PMID:18598360

  4. Role of IGF-1/IGF-1R in regulation of invasion in DU145 prostate cancer cells

    Directory of Open Access Journals (Sweden)

    Setya Hemani

    2008-07-01

    Full Text Available Abstract Background Prostate cancer progression to androgen independence is the primary cause of mortality by this tumor type. The IGF-1/IGF-1R axis is well known to contribute to prostate cancer initiation, but its contribution to invasiveness and the downstream signalling mechanisms that are involved are unclear at present. Results We examined the invasive response of androgen independent DU145 prostate carcinoma cells to IGF-1 stimulation using Matrigel assays. We then examined the signaling mechanisms and protease activities that are associated with this response. IGF-1 significantly increased the invasive capacity of DU145 cells in vitro, and this increase was inhibited by blocking IGF-1R. We further demonstrated that specific inhibitors of the MAPK and PI3-K pathways decrease IGF-1-mediated invasion. To determine potential molecular mechanisms for this change in invasive capacity, we examined changes in expression and activity of matrix metalloproteinases. We observed that IGF-1 increases the enzymatic activity of MMP-2 and MMP-9 in DU145 cells. These changes in activity are due to differences in expression in the case of MMP-9 but not in the case of MMP-2. This observation is corroborated by the fact that correlated changes of expression in a regulator of MMP-2, TIMP-2, were also seen. Conclusion This work identifies a specific effect of IGF-1 on the invasive capacity of DU145 prostate cancer cells, and furthermore delineates mechanisms that contribute to this effect.

  5. c-Met inhibitor SU11274 enhances the response of the prostate cancer cell line DU145 to ionizing radiation

    Energy Technology Data Exchange (ETDEWEB)

    Yu, Hongliang; Li, Xiaoying; Sun, Shaoqian [Department of Radiation Oncology, Peking University First Hospital, Peking University, Beijing (China); Gao, Xianshu, E-mail: xsgao777@hotmail.com [Department of Radiation Oncology, Peking University First Hospital, Peking University, Beijing (China); Zhou, Demin, E-mail: deminzhou@bjmu.edu.cn [State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing (China)

    2012-10-26

    Highlights: Black-Right-Pointing-Pointer c-Met inhibition could significantly enhance the radiosensitivity of DU145 cells. Black-Right-Pointing-Pointer The mechanisms of the radiosensitization effect of c-Met inhibition on DU145 cells were also presented in this paper. Black-Right-Pointing-Pointer This is the first study demonstrating the effectiveness of c-Met inhibition on treating HRPC cells with radiotherapy. -- Abstract: Hormone-refractory prostate cancer shows substantial resistance to most conventional therapies including radiotherapy, constitutes a key impediment to curing patients with the disease. c-Met overexpression plays a key role in prostate cancer tumorigenesis and disease progression. Here, we demonstrate that c-Met inhibition by SU11274 could significantly suppress cell survival and proliferation as well as enhance the radiosensitivity of DU145 cells. The underlying mechanisms of the effects of SU11274 on DU145 cells may include the inhibition of c-Met signaling, depolarization of the mitochondrial membrane potential, impairment of DNA repair function, abrogation of cell cycle arrest, and enhancement of cell death. Our study is the first to show the effectiveness of combining c-Met inhibition with ionizing radiation to cure hormone-refractory prostate cancer.

  6. Differential requirement for MEK Partner 1 in DU145 prostate cancer cell migration

    Directory of Open Access Journals (Sweden)

    Bailey Evangeline M

    2009-11-01

    Full Text Available Abstract ERK signaling regulates focal adhesion disassembly during cell movement, and increased ERK signaling frequently contributes to enhanced motility of human tumor cells. We previously found that the ERK scaffold MEK Partner 1 (MP1 is required for focal adhesion disassembly in fibroblasts. Here we test the hypothesis that MP1-dependent ERK signaling regulates motility of DU145 prostate cancer cells. We find that MP1 is required for motility on fibronectin, but not for motility stimulated by serum or EGF. Surprisingly, MP1 appears not to function through its known binding partners MEK1 or PAK1, suggesting the existence of a novel pathway by which MP1 can regulate motility on fibronectin. MP1 may function by regulating the stability or expression of paxillin, a key regulator of motility.

  7. Du travailleur au malade : retour sur la reconstitution du parcours professionnel de patients atteints de cancer en Seine-Saint-Denis

    Directory of Open Access Journals (Sweden)

    Flaviene Lanna

    2013-03-01

    Full Text Available Le Groupement d’intérêt scientifique sur les cancers d’origine professionnelle en Seine Saint Denis – Giscop93 – mène depuis 2002 une enquête permanente sur les expositions aux cancérogènes dans l’activité de travail et sur la reconnaissance de ces cancers comme maladies professionnelles. La première étape de l’enquête consiste en une reconstitution fine du parcours professionnel du patient par un entretien qualitatif dont le compte-rendu sera analysé par un collège d’experts afin d’identifier des éventuelles expositions. Cet article s’intéresse à la méthode utilisée pour reconstituer les parcours professionnels et cherche à montrer quelques unes des dimensions sociales jouant un rôle dans la construction du récit que livre le patient.Since 2002, Giscop93 (working out of Seine Saint Denis, a northern suburb of Paris has conducted an ongoing survey into occupational exposure to carcinogens and whether these cancers qualify for workers’ compensation. The first stage consists of a subtle reconstruction of a patient’s job history based on a qualitative interview analyzed subsequently by industrial health experts looking to identify possible exposures. The article also looks at the method used to reconstruct individual job histories and tries to show some of the social dimensions that play a role in constructing a patient’s narrative.El Grupo de interés científico sobre los cánceres de origen profesional en Seine-Saint-Denis (Giscop93 realiza desde 2002 un estudio permanente sobre las exposiciones a los cancerígenos en la actividad laboral y sobre el reconocimiento de esos cánceres como enfermedades profesionales. La primera etapa del estudio consiste en una reconstitución fina de la trayectoria profesional del paciente a través de una entrevista cualitativa tras la cual se elabora un informe que es analizado por un colegio de expertos con el fin de identificar eventuales exposiciones. En este art

  8. Breast cancer with axillary lymph node involvement; Cancer du sein avec atteinte ganglionnaire axillaire

    Energy Technology Data Exchange (ETDEWEB)

    Belaid, A.; Kanoun, S.; Kallel, A.; Ghorbel, I.; Azoury, F.; Heymann, S.; Marsiglia, H.; Bourgier, C. [Departement de radiotherapie, Unite fonctionnelle de Senologie, institut Gustave-Roussy, 94 - Villejuif (France); Belaid, A.; Ghorbel, I. [Service de radiotherapie Carcinologique, institut Salah-Azaiez, Tunis (Tunisia); Kanoun, S. [Service de radiotherapie, hopital Farhat-Hached, Sousse (Tunisia); Kallel, A. [dUnite de radiotherapie, clinique Ennasr (Tunisia); Pichenot, C.; Verstraet, R. [Departement de physique, institut Gustave-Roussy, 94 - Villejuif (France); Marsiglia, H. [Universite de Florence (Italy)

    2010-07-01

    Breast cancer is the most frequent cancer of women in western countries. There are one million new cases per year in the world which represents 22% of all female cancers, and more than 370.000 deaths due to breast cancer per year (14% of cancer mortality). More than half of breast cancers are associated with axillary nodal involvement. Post-operative radiation therapy (XRT) is a crucial part of locoregional treatment in axillary nodal involvement breast cancer owing to a 15-years risk reduction of locoregional recurrence of 70% and to a 5.4% risk reduction of specific mortality. In 3D-conformal irradiation in such breast cancers, target volumes are chest wall when mastectomy was performed or breast and boost of tumor bed in case of breast conservative surgery, and supra-clavicular and/or axillary and/or internal mammary node areas. The main organs at risk are ipsilateral lung, heart and brachial plexus. The aim of this article is to describe epidemiologic, radio anatomic and prognostic features of axillary nodal involvement breast cancer and to propose guidelines for 3D-conformal treatment planning in locally advanced breast cancers. This review is illustrated by a case report. (authors)

  9. Capsaicin-induced genotoxic stress does not promote apoptosis in A549 human lung and DU145 prostate cancer cells.

    Science.gov (United States)

    Lewinska, Anna; Jarosz, Paulina; Czech, Joanna; Rzeszutek, Iwona; Bielak-Zmijewska, Anna; Grabowska, Wioleta; Wnuk, Maciej

    2015-02-01

    Capsaicin is the major pungent component of the hot chili peppers of the genus Capsicum, which are consumed worldwide as a food additive. More recently, the selective action of capsaicin against cancer cells has been reported. Capsaicin was found to induce apoptosis and inhibit proliferation of a wide range of cancer cells in vitro, whereas being inactive against normal cells. As data on capsaicin-induced genotoxicity are limited and the effects of capsaicin against human lung A549 and DU145 prostate cancer cells were not explored in detail, we were interested in determining whether capsaicin-associated genotoxicity may also provoke A549 and DU145 cell death. Capsaicin-induced decrease in metabolic activity and cell proliferation, and changes in the cell cycle were limited to high concentrations used (≥ 100 μM), whereas, at lower concentrations, capsaicin stimulated both DNA double strand breaks and micronuclei production. Capsaicin was unable to provoke apoptotic cell death when used up to 250 μM concentrations. Capsaicin induced oxidative stress, but was ineffective in provoking the dissipation of the mitochondrial inner transmembrane potential. A different magnitude of p53 binding protein 1 (53BP1) recruitment contributed to diverse capsaicin-induced genotoxic effects in DU145 and A549 cells. Capsaicin was also found to be a DNA hypermethylating agent in A549 cells. In summary, we have shown that genotoxic effects of capsaicin may contribute to limited susceptibility of DU145 and A549 cancer cells to apoptosis in vitro, which may question the usefulness of capsaicin-based anticancer therapy, at least in a case of lung and prostate cancer.

  10. Regulation of DU145 prostate cancer cell growth by Scm-like with four mbt domains 2.

    Science.gov (United States)

    Lee, Kwanghyun; Na, Wonho; Maeng, Je-Heon; Wu, Hongjin; Ju, Bong-Gun

    2013-03-01

    Mammalian SFMBTs have been considered to be polycomb group repressors. However, molecular mechanisms underlying mammalian SFMBTs-mediated gene regulation and their biological function have not been characterized. In the present study, we identified YY1 and methylated histones as interacting proteins of human SFMBT2. We also found that human SFMBT2 binds preferentially to methylated histone H3 and H4 that are associated with transcriptional repression. Using DU145 prostate cancer cells as a model, we showed that SFMBT2 has a transcriptional repression activity on HOXB13 gene expression. In addition, occupancy of SFMBT2 coincided with enrichment of diand tri-methylated H3K9 and H4K20 as well as tri-methylated H3K27 at the HOXB13 gene promoter. When SFMBT2 was depleted by siRNA in DU145 prostate cancer cells, significant up-regulation of HOXB13 gene expression and decreased cell growth were observed. Collectively, our findings indicate that human SFMBT2 may regulate cell growth via epigenetic regulation of HOXB13 gene expression in DU145 prostate cancer cells.

  11. Regulation of DU145 prostate cancer cell growth by Scm-like with four mbt domains 2

    Indian Academy of Sciences (India)

    Kwanghyun Lee; Wonho Na; Je-Heon Maeng; Hongjin Wu; Bong-Gun Ju

    2013-03-01

    Mammalian SFMBTs have been considered to be polycomb group repressors. However, molecular mechanisms underlying mammalian SFMBTs-mediated gene regulation and their biological function have not been characterized. In the present study, we identified YY1 and methylated histones as interacting proteins of human SFMBT2. We also found that human SFMBT2 binds preferentially to methylated histone H3 and H4 that are associated with transcriptional repression. Using DU145 prostate cancer cells as a model, we showed that SFMBT2 has a transcriptional repression activity on HOXB13 gene expression. In addition, occupancy of SFMBT2 coincided with enrichment of diand tri-methylated H3K9 and H4K20 as well as tri-methylated H3K27 at the HOXB13 gene promoter. When SFMBT2 was depleted by siRNA in DU145 prostate cancer cells, significant up-regulation of HOXB13 gene expression and decreased cell growth were observed. Collectively, our findings indicate that human SFMBT2 may regulate cell growth via epigenetic regulation of HOXB13 gene expression in DU145 prostate cancer cells.

  12. A partner monoclonal antibody to Moab 730 kills 100% of DU145 and PC3 androgen-independent cancer cells

    Indian Academy of Sciences (India)

    Hemant Kumar Vyas; Rahul Pal; Nirmal K Lohiya; G P Talwar

    2009-12-01

    A number of therapeutic options are available for patients with prostate carcinoma till the time that the tumour is hormone dependent. However, no fully effective therapy is available for the treatment of androgen-independent prostate carcinomas. Antibodies directed at epitopes unique to or overexpressed on the cancer cells could be of therapeutic utility. A monoclonal antibody (Moab) 2C4 has been generated, which binds with cells of two androgenindependent prostate cancers, DU145 and PC3, and does not bind to peripheral blood leukocytes (PBLs) of healthy donors. This antibody, along with the previously developed Moab 730, kills 100% of both DU145 and PC3 cells in the presence of complement and does not have a deleterious effect on PBLs of healthy males. The anti-tumour action of the two antibodies prevents the establishment of DU145 cell tumour in nude mice in vivo. Moab 2C4 in combination with 730 has potential for use as therapy for androgen-independent cancers.

  13. Le tamoxifene comme premier traitement du cancer du sein chez la femme âgée: un recul de 10 ans

    OpenAIRE

    Colin, Claude; Lifrange, Eric; Genicot, F.; Castronovo, Vincenzo; Lambotte, René

    1990-01-01

    Cinquante-six patientes, âgées de plus de 70 ans, ont été traitées par tamoxifène pour cancer mammaire T1 à T4. Un délai de 3 à 6 mois est nécessaire pour obtenir une régression de l'opacité tumorale dans 46,4% des cas, une stabilisation dans 30,4% et une augmentation dans 23,2%. Le type de réponse semble indépendant du stade initial de la tumeur. La survie globale à 10 ans n'est pas différente de cette des patientes de même âge qui avaient préféré recourir au traitement loco-régional class...

  14. Amygdalin induces apoptosis through regulation of Bax and Bcl-2 expressions in human DU145 and LNCaP prostate cancer cells.

    Science.gov (United States)

    Chang, Hyun-Kyung; Shin, Mal-Soon; Yang, Hye-Young; Lee, Jin-Woo; Kim, Young-Sick; Lee, Myoung-Hwa; Kim, Jullia; Kim, Khae-Hawn; Kim, Chang-Ju

    2006-08-01

    Prostate cancer is one of the most common non-skin cancers in men. Amygdalin is one of the nitrilosides, natural cyanide-containing substances abundant in the seeds of plants of the prunasin family that have been used to treat cancers and relieve pain. In particular, D-amygdalin (D-mandelonitrile-beta-D-gentiobioside) is known to exhibit selective killing effect on cancer cells. Apoptosis, programmed cell death, is an important mechanism in cancer treatment. In the present study, we prepared the aqueous extract of the amygdalin from Armeniacae semen and investigated whether this extract induces apoptotic cell death in human DU145 and LNCaP prostate cancer cells. In the present results, DU145 and LNCaP cells treated with amygdalin exhibited several morphological characteristics of apoptosis. Treatment with amygdalin increased expression of Bax, a pro-apoptotic protein, decreased expression of Bcl-2, an anti-apoptotic protein, and increased caspase-3 enzyme activity in DU145 and LNCaP prostate cancer cells. Here, we have shown that amygdalin induces apoptotic cell death in human DU145 and LNCaP prostate cancer cells by caspase-3 activation through down-regulation of Bcl-2 and up-regulation of Bax. The present study reveals that amygdalin may offer a valuable option for the treatment of prostate cancers.

  15. Cancers du sein bilatéraux synchrones et grossesse a l'institut Joliot Curie de Dakar (Sénégal)

    Science.gov (United States)

    Zongo, Nayi; Sawadogo, Yobi Alexis; Some, Some Ollo Roland; Bagre, Sidpawalmdé Carine; Ka, Sidy; Diouf, Doudou; Dieng, Mamadou Moustapha; Gaye, Papa Macoumba; Dem, Ahmadou

    2016-01-01

    Décrire notre stratégie diagnostique et thérapeutique dans les cancers du sein bilatéraux synchrones pendant la grossesse. L’âge gestationnel au moment du diagnostic du cancer était respectivement de 7; 21 et 25 semaines. Il s'agissait de stade IV et IIIA respectivement dans deux et un cas. Elles ont toutes bénéficié d'une chimiothérapie dans deux cas pendant la grossesse (6TEC et 3AC) et dans un cas après l'accouchement. Une mastectomie bilatérale a été réalisée dans un cas. Une patiente est décédée. Les autres étaient vivantes mais toutes métastatiques. Une hypotrophie fœtale a été notée dans un cas. Le diagnostic du cancer est tardif dans cette association. La chirurgie est faisable et le type de chirurgie serait seulement fonction du stade du cancer. La chimiothérapie est possible avec moins de complications fœtales aux deux derniers trimestres. PMID:27279967

  16. Pentamidine reduces expression of hypoxia-inducible factor-1α in DU145 and MDA-MB-231 cancer cells.

    Science.gov (United States)

    Jung, Hui-Jung; Suh, Seong-Il; Suh, Min-Ho; Baek, Won-Ki; Park, Jong-Wook

    2011-04-01

    Pentamidine is an aromatic diamine used for the treatment of human protozoa infections. Recently, pentamidine has been reported to exhibit anticancer properties. In this study, we report that pentamidine inhibits expression of hypoxia-inducible factor (HIF)-1α in cancer cells. Pentamidine decreased HIF-1α protein translation and enhanced its protein degradation in DU145 prostate cancer and MDA-MB-231 breast cancer cells. In parallel with reduction of de novo synthesis of HIF-1α, pentamidine was able to suppress global protein translation, an effect accompanied by the reduction of eIF4F complex formation and also the induction of eIF2α phosphorylation. These results show that pentamidine is a potential inhibitor of HIF-1α and its potential as a cancer therapeutic reagent warrants further study.

  17. Métastase osseuse isolée du radius métachrone d’un cancer rectal

    Science.gov (United States)

    Eddekkaoui, Houda; Chekrine, Tarik; Sahraoui, Souha; Marouane, Sofia; Alj, Amina; Zamiati, Soumaya; Nechad, Mohamed; Benider, Abdellatif

    2013-01-01

    Les métastases osseuses isolées des cancers colorectaux sont très rares. Le squelette axial est habituellement le plus atteint. La localisation au niveau du radius est exceptionnelle. Nous rapportons l'observation d'une femme âgée de 60 ans avec une métastase du radius distal isolée métachrone d'un cancer du haut rectum opéré un an auparavant. La métastase a été découverte sur les examens d'imagerie et confirmée par biopsie. Une amputation a été réalisée suite à une progression de la maladie après une radiothérapie palliative. L'évolution était marquée par l'apparition de métastases pulmonaires et le décès est survenu dans un tableau de défaillance respiratoire. PMID:23819003

  18. L'invisibilité du cancer en Afrique [Review of: J. Livingston Improvising medicine: an African oncology ward in an emerging cancer epidemic

    NARCIS (Netherlands)

    Chabrol, F.; Nguyen, V.K.

    2013-01-01

    L’Afrique australe se remet à peine de l’épidémie de sida, grâce à une mobilisation transnationale sans précédent et à des financements massifs pour acheminer les antirétroviraux, qu’une épidémie de cancer prolifère dans le sillon du VIH. Comment rendre compte de ce drame oublié des politiques de sa

  19. Prostate cancer radiotherapy in elderly person; Radiotherapie du cancer de la prostate chez la personne agee

    Energy Technology Data Exchange (ETDEWEB)

    Serre, A. [Centre Alexis-Vautrin, Nancy (France)

    2011-10-15

    The author discusses the issue of prostate cancer radiotherapy in the case of elderly persons. The choice of the therapeutic strategy (local, hormonotherapy, simple monitoring) is complex. Different aspects must be considered: the carcinologic situation assessment, the patient health condition, the patient life expectancy, and the possible side effects of treatment. Radiotherapy appears to be a major therapeutic asset, but dose levels, toxicity effects must then be considered. Short communication

  20. Radiotherapy for cutaneous cancers with xeroderma pigmentosum; Radiotherapie des cancers cutanes au cours du xeroderma pigmentosum

    Energy Technology Data Exchange (ETDEWEB)

    Ben Salah, H.; Bahri, M.; Turki, H.; Abdelmoula, M.; Frikha, M.; Daoud, J. [Service de radiotherapie, CHU Habib-Bourguiba, route Majida-Bouleila, 3029 Sfax (Tunisia)

    2011-08-15

    Purpose. - To analyze the therapeutic results of cutaneous cancers on xeroderma pigmentosum through a series of 15 patients treated by radiotherapy. Patients and methods. - Between 1993 and 2006, 15 patients with xeroderma pigmentosum and having cutaneous cancers were treated in the Radiotherapy Department of university hospital Habib-Bourguiba of Sfax in Tunisia. Seventy-three percent of the cases occurred in male patients and the mean age of appearance of the first tumour was 18.2 years. Tumour histology was squamous cell carcinoma in 74% of the cases. The total number of cutaneous tumours was 84. Ten patients had a surgical resection. Four patients did not respond to chemotherapy. The modality of irradiation was decided according to the size, thickness and localization of the tumour. The dose of radiotherapy was 60 Gy or equivalent with classic irradiation. Results. - The total number of lesions treated with radiotherapy was 64. Forty-three lesions were treated with contact-therapy, ten with brachytherapy and 11 with cobalt-therapy. The following acute complications were observed: cutaneous infection (53.3% of patients), radio-epithelitis (80% of patients) and necroses (33.3% of patients). Evaluation after treatment showed a clinical complete remission in 73% of the cases. Late effects were noted in seven cases: telangiectasia and cutaneous atrophy. A recurrence in the irradiated zone was observed in one case. A nodal metastasis was observed in two cases. Another patient presented lung metastases. After a median follow up of 37.2 months, four patients died, seven are alive with cutaneous cancer and four are alive with complete remission. Conclusion. - Radiotherapy is a possible and effective therapeutic alternative. Dose and methods are not defined for xeroderma pigmentosum. (authors)

  1. Great potential of a panel of multiple hMTH1, SPD, ITGA11 and COL11A1 markers for diagnosis of patients with non-small cell lung cancer.

    Science.gov (United States)

    Chong, Inn-Wen; Chang, Mei-Yin; Chang, Huang-Chou; Yu, Ya-Ping; Sheu, Chau-Chyun; Tsai, Jong-Rung; Hung, Jen-Yu; Chou, Shah-Hwa; Tsai, Mee-Sun; Hwang, Jhi-Jhu; Lin, Shiu-Ru

    2006-11-01

    Research on molecular mechanisms underlying the carcinogenesis of non-small cell lung cancer (NSCLC) may provide gene targets in critical pathways valuable for improving the efficacy of therapy and survival of patients with NSCLC. However, the molecular markers highly sensitive for the prognosis and treatment evaluation of NSCLC are not yet available. To explore candidates, we conducted an oligonucleotide microarray study with three pairs of NSCLC and normal lung tissue, and determined 8 differentially expressed genes including the Human MutT homologue (hMTH1), Surfactant protein D (SPD), Human hyaluronan binding protein 2 (HABP2), Crystalline-mu (CRYM), Ceruloplasmin (CP), Integrin alpha-11 subunit (ITGA11), Collagen type XI alpha I (COL11A1), and Lung-specific X protein (Lun X). Four lung cancer-related markers MUC-1, hTERT, hnRNP B1, and CK-19 were also incorporated for further analysis. The expression profiles of the twelve genes in seventy pairs of NSCLC tumor and normal lung tissue were then detected quantitatively by using membrane array and quantitative real-time PCR (qRT-PCR). The data of the membrane array and qRT-PCR were compared for consistency and the potential of these mRNA markers in clinical application. The results showed that membrane array and qRT-PCR obtained consistent data for the tested genes in both sensitivity and specificity (correlation coefficient 0.921, p<0.0001). For patients' clinicopathological characteristics, the overexpression of hMTH1, SPD, HABP 2, ITGA11, COL11A1, and CK-19 was significantly correlated with the pathological stage (p<0.05). In addition, the overexpression of hMTH1, SPD, ITGA11, and COL11A1 was correlated with lymph node metastasis and poor prognosis. This is the first report relating SPD to a prognosis marker for NSCLC. Moreover, the combined detection of these four mRNA markers by membrane array had a sensitivity of 89% and a specificity of 84% for NSCLC, significantly higher than these markers had achieved

  2. Preoperative concurrent chemo-radiation in rectal cancer; Radiochimiotherapie concomitante preoperatoire pour cancer du rectum

    Energy Technology Data Exchange (ETDEWEB)

    Berger, C.; Kirscher, S.; Felix-Faure, C.; Chauvet, B.; Vincent, P.; Brewer, Y.; Reboul, F. [Clinique Sainte-Catherine, 84 - Avignon (France)

    1998-05-01

    To evaluate retrospectively treatment-related morbidity of concurrent radiotherapy and chemotherapy for rectal cancer. Between 1992 and 1995, 38 patients (median age: 60) were treated for locally advanced resectable rectal cancer. Median dose of radiotherapy was 45 Gy/25 fractions/5 weeks. Chemotherapy consisted of two courses of 5-fluorouracil and leucovorin administered during the first and the fifth weeks of radiotherapy. Median dose of 5-fluorouracil was 350 mg/m{sup 2}/day, and median dose of leucovorin was 350 mg/m{sup 2}/day, day 1 to day 5. Surgery was performed 5 weeks after completion of radiotherapy. Before surgery, one patient died of febrile neutropenia and sepsis after two cycles of chemotherapy and 45 Gy. Main pre-operative grade 3-4 toxicities were respectively: neutropenia: 3% ; nausea/vomiting: 3%; diarrhea: 3%; proctitis: 5%; radiation dermatitis: 8%. Twenty-six patients underwent a low anterior resection and 11 an abdomino-perineal resection. A temporary colostomy was performed in 12 patients. Pathologic complete response rate was 27 %. There was one post-operative death due to thrombo-embolic disease. Major post-operative grade 3-4 complications were: pelvic infection: 14 %; abdominal infection : 5%; perineal sepsis: 8%; anastomotic dehiscence: 8%; cardiac failure: 5%. Delayed perineal wound healing was observed in six patients. No significant prognostic factor of post-operative complications has been observed. Median duration of hospitalization was 22 days. With a median follow-up of 24 months, 2-year overall and disease-free survival rates were 82 and 64%. Tolerance of preoperative concurrent chemoradiotherapy was acceptable. Ongoing controlled studies will assess the impact of this combined treatment on survival. (authors)

  3. Effects of miR-200c on the migration and invasion abilities of human prostate cancer Du145 cells and the corresponding mechanism.

    Science.gov (United States)

    Shi, Runlin; Xiao, Haibing; Yang, Tao; Chang, Lei; Tian, Yuanfeng; Wu, Bolin; Xu, Hua

    2014-12-01

    microRNAs (miRNAs) have played a key role in human tumorigenesis, tumor progression, and metastasis. On the one hand, miRNAs are aberrantly expressed in many types of human cancer; on the other hand, miRNAs can function as tumor suppressors or oncogenes that target many cancer-related genes. This study aimed to investigate the effects of miRNA-200c (miR-200c) on the biological behavior and mechanism of proliferation, migration, and invasion in the prostate cancer cell line Du145. In this study, Du145 cells were transfected with miR-200c mimics or negative control miR-NC by using an X-tremeGENE siRNA transfection reagent. The relative expression of miR-200c was measured by RT-PCR. The proliferation, migration, and invasion abilities of Du145 cells were detected by CCK8 assays, migration assays and invasion assays, respectively. The expressions of ZEB1, E-cadherin, and vimentin were observed by western blot. Results showed that DU145 cells exhibited a high expression of miR-200c compared with immortalized normal prostate epithelial cell RWPE-1. Du145 cells were then transfected with miR-200c mimics and displayed lower abilities of proliferation, migration, and invasion than those transfected with the negative control. The protein levels of ZEB1 and vimentin were expressed at a low extent in Du145 cells, which were transfected with miR-200c mimics; by contrast, E-cadherin was highly expressed. Hence, miR-200c could significantly inhibit the proliferation of the prostate cancer cell line Du145; likewise, miR-200c could inhibit migration and invasion by epithelial-mesenchymal transition.

  4. GPNMB/OA protein increases the invasiveness of human metastatic prostate cancer cell lines DU145 and PC3 through MMP-2 and MMP-9 activity

    Energy Technology Data Exchange (ETDEWEB)

    Fiorentini, Chiara; Bodei, Serena; Bedussi, Francesca; Fragni, Martina; Bonini, Sara Anna [Section of Pharmacology, Department of Molecular and Translational Medicine, University of Brescia, V.le Europa 11, 25124 Brescia (Italy); Simeone, Claudio; Zani, Danilo [Division of Urology, Department of Surgery, Radiology and Public Health, University of Brescia, P.le Spedali Civili 1, 25124 Brescia (Italy); Berruti, Alfredo [Medical Oncology, Department of Surgery, Radiology, and Public Health, University of Brescia, P.le Spedali Civili 1, 25124 Brescia (Italy); Missale, Cristina; Memo, Maurizio; Spano, PierFranco [Section of Pharmacology, Department of Molecular and Translational Medicine, University of Brescia, V.le Europa 11, 25124 Brescia (Italy); Sigala, Sandra, E-mail: sigala@med.unibs.it [Section of Pharmacology, Department of Molecular and Translational Medicine, University of Brescia, V.le Europa 11, 25124 Brescia (Italy)

    2014-04-15

    Non-metastatic glycoprotein melanoma protein B (GPNMB), also known as osteoactivin (OA) is expressed in a wide array of tumors and represents an emerging target for drug development. In this study, we investigated the role of GPNMB/OA in the progression of human metastatic DU145 and PC3 prostate cancer cells. GPNMB/OA contribution in PCa malignant phenotype has been analyzed by small interfering RNA-induced GPNMB/OA silencing. We found that following GPNMB/OA silencing the migration capability of both DU145 and PC3 cells, evaluated by using in vitro invasivity assay, as well as the metalloproteinases MMP-2 and MMP-9 activity were equally strongly inhibited. By contrast knocking down GPNMB/OA weakly attenuated cell proliferation rate of DU145, an effect that paralleled with an increase number of apoptotic cells. However, PC3 cell growth seems to be not affected by GPNMB/OA. Together, these data reveal that GPNMB/OA acts as a critical molecular mediator promoting the acquisition of the more aggressive, pro-metastatic phenotype distinctive of human DU145 and PC3 cell lines. - Highlights: • GPNMB/OA expression correlates with DU145 and PC3 cells malignant phenotype. • GPNMB/OA silencing affects the migration capability of both DU145 and PC3 cells. • GPNMB/OA increases invasiveness by up-regulating MMPs activity. • GPNMB/OA promotes DU145 and PC3 cells progression into a more aggressive phenotype.

  5. Study on the mechanism of JianPiJieDu prescription promoting Oxaliplatin in the treatment of gastric cancer

    Institute of Scientific and Technical Information of China (English)

    Pei Xiang; Yi-Jiao Huang; Wei-Min Zhu

    2016-01-01

    Objective:To explore the mechanism of JianPiJieDu Prescription promoting oxaliplatin in the treatment of gastric cancer, to provide help for cancer chemotherapy.Methods:A total of 90 cases of middle gastric cancer patients in our hospital were randomly divided into into the observation group and the control group according to the random number table method, 45 cases in each group, patients of the control group were treated with oxaliplatin for two cycles, based on it, patients of the observation group were treated with JianPiJieDu decoction for two cycles .Compared the CEA, CA199 levels, immune cells and related immune factors content, blood routine, liver function and renal function index sign between the two groups before and after treatment .Results:There were no significant differences in the indexes between the two groups before treatment; after JianpiJiedu decoction combined with oxaliplatin treatment, tumor markers CEA and CA199 in observation group were significantly decreased compared with the control group; after treatment, cytokines in observation group was significantly improved compared with the control group , CD4+ and CD4+/CD8+ in observation group were significantly increased compared with the control group, there were statistically significant differences; before and after treatment, leucocyte and erythrocyte as well as blood platelet in observation group had no significant differences, blood routine indexes in control group decreased significantly after treatment; in comparsion with before treatment, liver function and renal function were significantly decreased in observation group patients after JianpiJiedu decoction combined with oxaliplatin treatment, while the indexes of the control group were significantly improved after chemotherapy.Conclusion: JianpiJiedu Decoction can improve the immunity of tumor patients and reduce the toxic and side effects of chemotherapy drugs, It is of great significance to improve the efficiency of chemotherapy

  6. 人前列腺癌细胞系DU145中肿瘤干细胞样侧群细胞的分离%Identification of cancer stem cell -like side population cells in human prostate cancer cell line DU 145

    Institute of Scientific and Technical Information of China (English)

    殷波; 刘岗; 张辉; 李明; 隋文印; 宋永胜

    2012-01-01

    Objective;To identify the side population (SP) cells from human prostate cancer cell line DU 145 and characterize DU 145 SP cells preliminary. Methods: Fluorescence activated cell sorting (FACS) was applied to isolate SP cells from DU 145 cell line. The isolated SP cells were cultured in serum - free medium to observe the growth pattern. Reverse transcription - polymerase chain reaction (RT - PCR) was used to detect the expression of ABCG2 in DU 145 SP cells and parental DU 145 cells. Results: SP cells were isolated from DU 145 cells by flow cytometry, with a proportion of 1.1%. When cultured in serum -free medium, SP cells showed a clustered growth pattern compared to the parental DU 145 cells. Furthermore, a higher expression of ABCG2 in SP cells was detected by RT -PCR in contrast to parental DU 145 cells. Conclusion; DU 145 cell line contained SP cells with cancer stem cell properties.%目的:分离人前列腺癌细胞系DU 145中的侧群(side population, SP)细胞,并初步分析其生物学特性.方法:采用荧光激活细胞分类(fluorescence activated cell sorting,FACS)技术,从DU 145细胞中分离出侧群细胞,并检测其比例;继而培养于无血清培养基中,观察其生长特性.采用反转录聚合酶链反应( RT-PCR)技术检测侧群细胞中ABCG2的表达水平.结果:DU 145细胞中存在含量极少的侧群细胞,比例约1.1%;培养于无血清培养基中成簇生长.和对应的母系DU 145细胞相比,DU 145侧群细胞的ABCG2表达增高.结论:人前列腺癌细胞系DU 145中存在具有肿瘤干细胞特性的侧群细胞.

  7. 菲律宾蛤仔酶解多肽抗前列腺癌DU-145细胞的活性研究%Study on Antiproliferative Activity of Ruditapes philippinarum Hydrolysate on Human Prostate Cancer DU-145 Cell

    Institute of Scientific and Technical Information of China (English)

    李连军; 徐律; 杨最素; 丁国芳; 孙瑜

    2013-01-01

    [Objective] To study Philippines clam enzymolysis peptide on prostate cancer DU-145 cells proliferation activity in vitro.[Method] Digestive enzyme was used to enzymolysis the Philippine clam guts,3 kD molecular weight cut-off membranes were used to fractionate the Ruditapes philippinarum hydrolysate.The anti-proliferation activity and the early apoptosis of Ruditapes philippinarum peptides in vitro were detected by MTT,inverted microscope and AO/EB double staining respectively.[Result] Determined by MTT method,different concentration of peptides to DU-145 cells had obvious growth inhibition.Cell apoptosis morphological feature was observed under AO/EB double staining and inverted microscope.[Conclusion] Ruditapes philippinarum under 3 kD enzyme solution of molecular weight with inhibition of DU-145 cells growth,induce prostate cancer cells apoptosis,and a concentration and time dependence.%[目的]探讨菲律宾蛤仔酶解多肽抗人前列腺癌DU-145细胞增殖的活性.[方法]对菲律宾蛤仔内脏进行酶解,经超滤截取3 kD以下的多肽,应用MTT法、倒置显微镜观察和AO/EB染色等方法检测其体外抗DU-145细胞的活性.[结果]MTT法检测结果表明不同浓度的多肽对DU-145细胞有明显的生长抑制作用.通过倒置显微镜观察和AO/EB荧光双染观察到DU-145细胞出现细胞凋亡的形态学特征.[结论]菲律宾蛤仔3 kD以下的多肽具有抑制DU-145细胞生长,呈浓度和时间依赖性,并诱导细胞凋亡作用.

  8. IMPLICATION DE CERTAINES MUTATIONS DANS LES GENES BRCA1 ET BRCA2 SUR LA PRÉDISPOSITION AU CANCER DU SEIN ET AU CANCER OVARIEN

    Directory of Open Access Journals (Sweden)

    Lucian Negura

    2007-08-01

    Full Text Available Le cancer du sein, ainsi que celui ovarien, est une maladie fréquente chez les femmes, ayant un traitement assez difficile et, malheureusement, de sérieuses répercutions sur le physique ; c’est pourquoi il s’avère essentiel que la maladie soit dépistée dès les phases précoces. La prédisposition génétique est responsable de 5% des cancers et de 25% des cas apparus avant l’age de 30 ans [Breast Cancer Linkage Consortium, 1997]. Nous présentons ici l’implication des gènes suppresseurs des tumeurs BRCA1 et BRCA2 sur cette prédisposition.

  9. Results of the second campaign of the breast cancer detection in the Finistere; Resultats de la deuxieme campagne de depistage du cancer du sein dans le Finistere

    Energy Technology Data Exchange (ETDEWEB)

    Muller, M.; Dupre, P.F.; Wong, N.; Pradier, O.; Collet, M. [Centre Hospitalier Universitaire, 29 - Brest (France); Foll, Y. [ADEC 29, Brest (France)

    2007-11-15

    Some number of quality indicators of the detection is to be improved: the rate of participation (R.E. > 70 %), the positive predictive value of the biopsy (R.E.=75 %) and the proportion of cancers detected without a ganglionic invasion (R.E. > 70 %). (N.C.)

  10. Management of inflammatory breast cancer after neo-adjuvant chemotherapy; Traitement locoregional du cancer du sein inflammatoire apres chimiotherapie neo-adjuvante

    Energy Technology Data Exchange (ETDEWEB)

    Abrous-Anane, S.; Daveau, C.; Dendale, R.; Campana, F.; Kirova, Y.; Fourquet, A.; Bollet, M.A. [Service d' onco-radiotherapie, institut Curie, 26, rue d' Ulm, 75005 Paris cedex 05 (France); Savignoni, A.; Gautier, C. [Service de biostatistique, institut Curie, 26, rue d' Ulm, 75248 Paris cedex 05 (France); Pierga, J.Y. [Service d' oncologie medicale, institut Curie, 26, rue d' Ulm, 75248 Paris cedex 05 (France); Reyal, F. [Service de chirurgie, institut Curie, 26, rue d' Ulm, 75248 Paris cedex 05 (France)

    2011-12-15

    Purpose. - To assess the benefit of breast surgery for inflammatory breast cancer. Patients and methods. - This retrospective series was based on 232 patients treated for inflammatory breast cancer. All patients received primary chemotherapy followed by either exclusive radiotherapy (118 patients, 51%) or surgery with or without radiotherapy (114 patients, 49%). The median follow-up was 11 years. Results. - The two groups were comparable apart from fewer tumors smaller than 70 mm (43% vs 33%, P = 0.003), a higher rate of clinical stage N2 (15% vs 5%, P = 0.04) and fewer histopathological grade 3 tumors (46% vs 61%, P < 0.05) in the no-surgery group. The addition of surgery was associated with a significant improvement in locoregional disease control (P = 0.04) but with no significant difference in overall survival rates or disease-free intervals. Late toxicities were not significantly different between the two treatment groups except for a higher rate of fibrosis in the no-surgery group (P < 0.0001), and more lymphedema in the surgery group (P = 0.002). Conclusion. - Our data suggest an improvement in locoregional control in patients treated by surgery, in conjunction with chemotherapy and radiotherapy, for inflammatory breast cancer. (authors)

  11. About 13 cases of medullary metastatic compression in breast cancer; A propos de 13 cas de compression medullaire metastatique du cancer du sein

    Energy Technology Data Exchange (ETDEWEB)

    Mahfoud, T.; Aassab, R.; Errihani, H. [Service d' oncologie medicale, Institut national d' oncologie, Rabat (Morocco); Mahfoud, T.; Khmamouche, M.R.; Debbagh, A.; Bazine, A.; Ichou, M. [Service d' oncologie medicale, Hopital militaire d' instruction Mohammed-V, Rabat (Morocco)

    2010-10-15

    As this issue is a diagnosis and therapeutic emergency, the authors report the development of a method to take into care medullary metastatic compression of the breast cancer. This study is based on 13 patients of a Rabat hospital over 3 years. Compressions have been observed by MRI. They have been treated by cortico-therapy and radiotherapy, but also by laminectomy in one case. The authors observed a improvement in terms of symptomatology, and a neurological recovery from the fourth week after the treatment. Short communication

  12. The E1 copper binding domain of full-length amyloid precursor protein mitigates copper-induced growth inhibition in brain metastatic prostate cancer DU145 cells.

    Science.gov (United States)

    Gough, Mallory; Blanthorn-Hazell, Sophee; Delury, Craig; Parkin, Edward

    2014-10-31

    Copper plays an important role in the aetiology and growth of tumours and levels of the metal are increased in the serum and tumour tissue of patients affected by a range of cancers including prostate cancer (PCa). The molecular mechanisms that enable cancer cells to proliferate in the presence of elevated copper levels are, therefore, of key importance in our understanding of tumour growth progression. In the current study, we have examined the role played by the amyloid precursor protein (APP) in mitigating copper-induced growth inhibition of the PCa cell line, DU145. A range of APP molecular constructs were stably over-expressed in DU145 cells and their effects on cell proliferation in the presence of copper were monitored. Our results show that endogenous APP expression was induced by sub-toxic copper concentrations in DU145 cells and over-expression of the wild-type protein was able to mitigate copper-induced growth inhibition via a mechanism involving the cytosolic and E1 copper binding domains of the full-length protein. APP likely represents one of a range of copper binding proteins that PCa cells employ in order to ensure efficient proliferation despite elevated concentrations of the metal within the tumour microenvironment. Targeting the expression of such proteins may contribute to therapeutic strategies for the treatment of cancers.

  13. The E1 copper binding domain of full-length amyloid precursor protein mitigates copper-induced growth inhibition in brain metastatic prostate cancer DU145 cells

    Energy Technology Data Exchange (ETDEWEB)

    Gough, Mallory, E-mail: m.gough1@lancaster.ac.uk; Blanthorn-Hazell, Sophee, E-mail: s.blanthorn-hazell@lancaster.ac.uk; Delury, Craig, E-mail: c.delury@lancaster.ac.uk; Parkin, Edward, E-mail: e.parkin@lancaster.ac.uk

    2014-10-31

    Highlights: • Copper levels are elevated in the tumour microenvironment. • APP mitigates copper-induced growth inhibition of DU145 prostate cancer (PCa) cells. • The APP intracellular domain is a prerequisite; soluble forms have no effect. • The E1 CuBD of APP is also a prerequisite. • APP copper binding potentially mitigates copper-induced PCa cell growth inhibition. - Abstract: Copper plays an important role in the aetiology and growth of tumours and levels of the metal are increased in the serum and tumour tissue of patients affected by a range of cancers including prostate cancer (PCa). The molecular mechanisms that enable cancer cells to proliferate in the presence of elevated copper levels are, therefore, of key importance in our understanding of tumour growth progression. In the current study, we have examined the role played by the amyloid precursor protein (APP) in mitigating copper-induced growth inhibition of the PCa cell line, DU145. A range of APP molecular constructs were stably over-expressed in DU145 cells and their effects on cell proliferation in the presence of copper were monitored. Our results show that endogenous APP expression was induced by sub-toxic copper concentrations in DU145 cells and over-expression of the wild-type protein was able to mitigate copper-induced growth inhibition via a mechanism involving the cytosolic and E1 copper binding domains of the full-length protein. APP likely represents one of a range of copper binding proteins that PCa cells employ in order to ensure efficient proliferation despite elevated concentrations of the metal within the tumour microenvironment. Targeting the expression of such proteins may contribute to therapeutic strategies for the treatment of cancers.

  14. COL4A3 expression correlates with pathogenesis, pathologic behaviors, and prognosis of gastric carcinomas.

    Science.gov (United States)

    Nie, Xiao-cui; Wang, Jian-Ping; Zhu, Wan; Xu, Xiao-yan; Xing, Ya-nan; Yu, Miao; Liu, Yun-peng; Takano, Yasuo; Zheng, Hua-chuan

    2013-01-01

    COL4A3 protein belongs to type IV collagen family and is closely linked to kidney diseases and cancer. To clarify the roles of COL4A3 in gastric carcinogenesis and subsequent progression, its expression was examined by immunohistochemistry on tissue microarrays containing gastric carcinomas, adjacent intestinal metaplasia, pure intestinal metaplasia, and gastritis. Gastric carcinoma tissue and cell lines were studied for COL4A3 expression by Western blotting and reverse transcription-polymerase chain reaction. We found that COL4A3 was differentially expressed in GES-1, AGS, BGC-823, GT-3 TKB, HGC-27, KATO-III, MGC-803, MKN28, MKN45, SCH, SGC-7901, and STKM-2 at both messenger RNA and protein levels. Carcinomas showed statistically lower COL4A3 expression than matched nonneoplastic mucosa (P carcinoma (P carcinoma than gastritis (P carcinomas regardless of invasion into the muscularis propria (P carcinomas showed COL4A3 expression. Kaplan-Meier analysis indicated that COL4A3 expression was negatively associated with a favorable prognosis of overall, advanced, and intestinal-type gastric carcinomas (P carcinoma. COL4A3 overexpression might be used as a marker of gastric intestinal metaplasia and mucinous and signet ring cell carcinoma.

  15. Cancer du sein, mécanismes de défense et psychose

    OpenAIRE

    Gimenez, Guy; Norbert, S; Antoni, Françoise

    1998-01-01

    International audience; Some severe diseases as cancer provoke into patients important defensive strategies. This work explores the specificity of defenses implemented in the case of two female psychotic patients suffering of breast cancer – a pranoiac and a schizophrenic – as well as the effetc produced on a hallucinatory and delusion constructions.; Certaines maladies somatiques graves, comme le cancer, provoquent des stratégies défensives importantes chez les patients. Ce travail explore l...

  16. Dans l'oeil du cyclotron quand la haute technologie défie le cancer

    CERN Document Server

    Demard, François

    2016-01-01

    Cet ouvrage retrace l'histoire du cyclotron Medicyc, ce pionnier relativement méconnu de la protonthérapie en France qui permit à Nice le premier traitement d'un mélanome de l'oeil au centre Antoine Lacassagne. Fruit d'une étroite collaboration entre l'ingénieur et le médecin et, dès l'origine, dévolu à la radiothérapie des tumeurs et à la production de radio-isotopes médicaux. Aujourd'hui, quelques vingt-cinq ans plus tard, cette protonthérapie connaît un essor considérable dans le monde.

  17. Observations sur la maturité sexuelle et la ponte du tourteau Cancer pagurus en Manche

    OpenAIRE

    Latrouite, Daniel; Noel, Philippe

    1993-01-01

    Peu d'observations sur le cycle reproducteur et la taille de maturité fonctionnelle du crabe tourteau en Manche ont été rapportées jusqu'à présent, probablement en raison de la non vulnérabilité des femelles ovigères. La présente étude repose sur les observations faites, trois années consécutivement, sur des femelles pêchées avant la période de ponte et placées dans des viviers en laboratoire. Après discussion sur les critères pertinents pour l'établissement de la taille moyenne de première m...

  18. Irradiation of low rectal cancers; Radiotherapie des carcinomes du bas rectum

    Energy Technology Data Exchange (ETDEWEB)

    Ardiet, J.M.; Coquard, R.; Romestaing, P.; Fric, D.; Baron, M.H.; Rocher, F.P.; Sentenac, I.; Gerard, J.P. [Centre Hospitalier Lyon-Sud, 69 -Pierre-Benite (France)

    1994-12-31

    The low rectal cancers are treated by anorectal amputation and pose the problem of the sphincter conservation. Some authors extend the clinical definition to developed injuries until 12 cm from the anal margin. The rectal cancer is a frequent tumour which remains serious. When the tumour is low, the treatment consists in an anorectal amputation with a permanent colostomy. The radical non preserving surgery is the usual treatment of these injuries. Until 1960 the rectal adenocarcinoma was considered as a radioresistant tumour because of the impossibility to deliver an enough dose to the tumour by external radiotherapy. But other studies showed that those lesions were radiosensitive and often radiocurable. The medical treatments haven`t yet demonstrated their efficiency in the treatment of the rectal cancer. We`ll study the radiotherapy in the treatment of the low rectal cancer, solely radiotherapy, radiosurgical associations. 32 refs., 5 tabs.

  19. Localisation des ganglions sentinelles au moyen de quantum dots. Application au cancer du sein

    OpenAIRE

    2009-01-01

    Lymph node (LN) status is the most important prognostic factor in breast cancer patients and a determinant predictor of recurrence and survival. In the treatment of operable breast cancers with a size inferior of 3 cm, the sentinel lymph node biopsy (SLNB) substitutes the axillary LN dissection. However, this technique requires the simultaneous use of a radiocolloid and a physiologic dye, which present several secondary effects. Thus, our strategy was to test in preclinical model the new fluo...

  20. Concomitant chemo-radiotherapy for the locally advanced rectum cancer; Chimioradiotherapie concomitante dans le cancer du rectum localement evolue

    Energy Technology Data Exchange (ETDEWEB)

    Haoui, M.; Aksil, N.; Boualga, K.; Moussaoui, D.; Ladj, O. [Service de radiotherapie-oncologie, centre anti-cancer, Blida (Algeria)

    2010-10-15

    The authors report a retrospective study which aimed at assessing the use of a concomitant chemo-radiotherapy, its tolerance and its feasibility in the case of a locally advanced rectum cancer. Based on data obtained among 62 patients presenting a rectum cancer, they analyse the results in terms of tolerance (cases of leukopenia, anemia, diarrhea, radiodermatitis), of relapses, and survival. Toxicity is acceptable and the concomitant treatment renders the tumour operable in many cases. Short communication

  1. Epidémiologie du cancer gastrique: expérience d'un centre hospitalier marocain

    Science.gov (United States)

    Mellouki, Ihsane; laazar, Nawal; Benyachou, Bahija; Aqodad, Nouredine; Ibrahimi, Adil

    2014-01-01

    Le cancer de l'estomac est représenté essentiellement par Les adénocarcinomes gastriques, ces derniers demeurent l'une des dix premières causes mondiales de mortalité avec un pronostic qui est péjoratif. Son incidence reste variable à travers le monde, elle est caractérisée par une importante disparité géographique. Le but de notre travail est de décrire les caractéristiques épidémiologiques de l'adénocarcinome gastrique dans notre contexte à travers une étude rétrospective, observationnelle étalée sur une période de 10 ans (Janvier 2001- Janvier 2011), incluant tous les malades admis au service d'hépato-gastroentérologie du CHU Hassan II de Fès pour prise en charge d'un adénocarcinome gastrique. Durant cette période, 343 patients étaient admis pour prise en charge d'une tumeur gastrique, dont 170 patients avaient un adénocarcinome gastrique (49.5%). L’âge moyen de ces patients était de 58±13.4 ans [16 ans-0 ans]. Dans 43.7% des cas, les patients provenaient de la région de Fès, souvent du milieu rurale. On note une nette prédominance masculine, avec une différence significative entre les 2 sexes (p gastrique (p = 0.02). la non consommation de l'alcool est inversement liée et de façon significative à l'apparition de l'adénocarcinome gastrique (p = 0.03) dans notre contexte. L'infection par Hélicobacter pylori n’était mentionnée que chez peu de malades. Les formes métastatiques au moment du diagnostic dépassaient 50% avec un taux de décès au cours de l'hospitalisation de 2.6%. Sur le plan endoscopique, la localisation antropylorique, et la forme ulcéro-végétante étaient prédominantes, elles présentaient successivement 49% (p = 0,002) et 66% (p = 0.00001). Une chirurgie curative n’était proposée que chez 50 patients (30.2%). L'adénocarcinome gastrique représente le type histologique le plus fréquent, son pronostic reste fâcheux dans notre région, touchant une population jeune, minimisant ainsi les

  2. Breast cancer in elderly person: which role for radiotherapy; Cancer du sein chez la personne agee: place de la radiotherapie?

    Energy Technology Data Exchange (ETDEWEB)

    Horn, S. [Centre Oscar-Lambret, Lille (France); Cutuli, B. [ICC Reims, Reims (France)

    2011-10-15

    The authors briefly discuss the issue and practices of treatment of elderly women suffering from breast cancer. Even if radiotherapy is sometimes forgotten in such cases, disease characteristics and the tolerance capacity of the patient must be considered before implementing treatment protocols. Short communication

  3. Therapeutic management of locally unresectable pancreatic cancer; Adenocarcinomes du pancreas localement evolues: modalites therapeutiques actuelles

    Energy Technology Data Exchange (ETDEWEB)

    Lombard-Bohas, C.; Saurin, J.C. [Centre Hospitalier Universitaire Edouard-Herriot, 69 - Lyon (France); Mornex, F. [Centre Hospitalier Universitaire Lyon-Sud, 69 - Pierre-Benite (France)

    1997-12-31

    Pancreatic cancer still have bad prognosis. At the time of diagnosis, less than 10 % of patients can undergo surgery with an overall 5-year survival rate of less than 2 %. For patients with localized pancreatic adenocarcinoma, the combination of radiation therapy and chemotherapy has been shown to control symptoms and to enhance patient survival. This treatment should be proposed to all the patients with good performance status and without icterus. Pain management should be optimized and often need morphinic and co-antalgic (anticonvulsants, steroids) consumption. The celiac plexus block with alcohol gives an excellent pain relief and should be more frequently used. (author)

  4. Participation du patient Marocain atteint du cancer au choix thérapeutique: résultat d'une enquête réalisée auprès de 272 patient

    OpenAIRE

    Boukir, Anwar; Azghari, Ilham; El Kabous, Mustapha; Jouid, Khalid; Boutayeb, Saber; El Ghissassi, Ibrahim; Mrabti, Hind; Errrihani, Hassan

    2015-01-01

    Introduction La décision médicale partagée ‘Shared decision making’ est un concept qui se développe depuis les années 1990. Il donne aux patients le soutien nécessaire pour exprimer leurs préférences et partager la décision médicale. Cette étude cherche à estimer le degré de participation du patient Marocain atteint de cancer au choix thérapeutique. Méthodes Cette enquête a été réalisée auprès de 272 malades sous chimiothérapie pour une pathologie cancéreuse sous forme d'un entretien verbal b...

  5. Plumbagin elicits differential proteomic responses mainly involving cell cycle, apoptosis, autophagy, and epithelial-to-mesenchymal transition pathways in human prostate cancer PC-3 and DU145 cells

    Directory of Open Access Journals (Sweden)

    Qui JX

    2015-01-01

    Full Text Available Jia-Xuan Qiu,1,2 Zhi-Wei Zhou, 3,4 Zhi-Xu He,4 Ruan Jin Zhao,5 Xueji Zhang,6 Lun Yang,7 Shu-Feng Zhou,3,4 Zong-Fu Mao11School of Public Health, Wuhan University, Wuhan, Hubei, People’s Republic of China; 2Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, People’s Republic of China; 3Department of Pharmaceutical Sciences, College of Pharmacy, University of South Florida, Tampa, FL, USA; 4Guizhou Provincial Key Laboratory for Regenerative Medicine, Stem Cell and Tissue Engineering Research Center and Sino-US Joint Laboratory for Medical Sciences, Guiyang Medical University, Guiyang, Guizhou, People’s Republic of China; 5Center for Traditional Chinese Medicine, Sarasota, FL, USA; 6Research Center for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing, People’s Republic of China; 7Bio-X Institutes, Key Laboratory for the Genetics of Development and Neuropsychiatric Disorders (Ministry of Education, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaAbstract: Plumbagin (PLB has exhibited a potent anticancer effect in preclinical studies, but the molecular interactome remains elusive. This study aimed to compare the quantitative proteomic responses to PLB treatment in human prostate cancer PC-3 and DU145 cells using the approach of stable-isotope labeling by amino acids in cell culture (SILAC. The data were finally validated using Western blot assay. First, the bioinformatic analysis predicted that PLB could interact with 78 proteins that were involved in cell proliferation and apoptosis, immunity, and signal transduction. Our quantitative proteomic study using SILAC revealed that there were at least 1,225 and 267 proteins interacting with PLB and there were 341 and 107 signaling pathways and cellular functions potentially regulated by PLB in PC-3 and DU145 cells, respectively. These proteins and pathways played a

  6. Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU 145 human prostate cancer cells

    Directory of Open Access Journals (Sweden)

    V. Vucic

    2006-02-01

    Full Text Available Gamma-irradiation (gamma-IR is extensively used in the treatment of hormone-resistant prostate carcinoma. The objective of the present study was to investigate the effects of 60Co gamma-IR on the growth, cell cycle arrest and cell death of the human prostate cancer cell line DU 145. The viability of DU 145 cells was measured by the Trypan blue exclusion assay and the 3(4,5-dimethylthiazol-2-yl-2,5,diphenyltetrazolium bromide test. Bromodeoxyuridine incorporation was used for the determination of cell proliferation. Cell cycle arrest and cell death were analyzed by flow cytometry. Superoxide dismutase (SOD, specifically CuZnSOD and MnSOD protein expression, after 10 Gy gamma-IR, was determined by Western immunoblotting analysis. gamma-IR treatment had a significant (P < 0.001 antiproliferative and cytotoxic effect on DU 145 cells. Both effects were time and dose dependent. Also, the dose of gamma-IR which inhibited DNA synthesis and cell proliferation by 50% was 9.7 Gy. Furthermore, gamma-IR induced cell cycle arrest in the G2/M phase and the percentage of cells in the G2/M phase was increased from 15% (control to 49% (IR cells, with a nonsignificant induction of apoptosis. Treatment with 10 Gy gamma-IR for 24, 48, and 72 h stimulated CuZnSOD and MnSOD protein expression in a time-dependent manner, approximately by 3- to 3.5-fold. These data suggest that CuZnSOD and MnSOD enzymes may play an important role in the gamma-IR-induced changes in DU 145 cell growth, cell cycle arrest and cell death.

  7. Pharmacoproteomic study of the natural product Ebenfuran III in DU-145 prostate cancer cells: the quantitative and temporal interrogation of chemically induced cell death at the protein level.

    Science.gov (United States)

    Roumeliotis, Theodoros I; Halabalaki, Maria; Alexi, Xanthippi; Ankrett, Dyan; Giannopoulou, Eugenia G; Skaltsounis, Alexios-Leandros; Sayan, Berna S; Alexis, Michael N; Townsend, Paul A; Garbis, Spiros D

    2013-04-05

    A naturally occurring benzofuran derivative, Ebenfuran III (Eb III), was investigated for its antiproliferative effects using the DU-145 prostate cell line. Eb III was isolated from Onobrychis ebenoides of the Leguminosae family, a plant endemic in Central and Southern Greece. We have previously reported that Eb III exerts significant cytotoxic effects on certain cancer cell lines. This effect is thought to occur via the isoprenyl moiety at the C-5 position of the molecule. The study aim was to gain a deeper understanding of the pharmacological effect of Eb III on DU-145 cell death at the translational level using a relative quantitative and temporal proteomics approach. Proteins extracted from the cell pellets were subjected to solution phase trypsin proteolysis followed by iTRAQ-labeling. The labeled tryptic peptide extracts were then fractionated using strong cation exchange chromatography and the fractions were analyzed by nanoflow reverse phase ultraperformance liquid chromatography-nanoelectrospray ionization-tandem mass spectrometry analysis using a hybrid QqTOF platform. Using this approach, we compared the expression levels of 1360 proteins analyzed at ≤ 1% global protein false discovery rate (FDR), commonly present in untreated (control, vehicle only) and Eb III-treated cells at the different exposure time points. Through the iterative use of Ingenuity Pathway Analysis with hierarchical clustering of protein expression patterns, followed by bibliographic research, the temporal regulation of the Calpain-1, ERK2, PAR-4, RAB-7, and Bap31 proteins were identified as potential nodes of multipathway convergence to Eb III induced DU-145 cell death. These proteins were further verified with Western blot analysis. This gel-free, quantitative 2DLC-MS/MS proteomics method effectively captured novel modulated proteins in the DU-145 cell line as a response to Eb III treatment. This approach also provided greater insight to the multifocal and combinatorial signaling

  8. Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU 145 human prostate cancer cells

    Directory of Open Access Journals (Sweden)

    Vucic V.

    2006-01-01

    Full Text Available Gamma-irradiation (gamma-IR is extensively used in the treatment of hormone-resistant prostate carcinoma. The objective of the present study was to investigate the effects of 60Co gamma-IR on the growth, cell cycle arrest and cell death of the human prostate cancer cell line DU 145. The viability of DU 145 cells was measured by the Trypan blue exclusion assay and the 3(4,5-dimethylthiazol-2-yl-2,5,diphenyltetrazolium bromide test. Bromodeoxyuridine incorporation was used for the determination of cell proliferation. Cell cycle arrest and cell death were analyzed by flow cytometry. Superoxide dismutase (SOD, specifically CuZnSOD and MnSOD protein expression, after 10 Gy gamma-IR, was determined by Western immunoblotting analysis. gamma-IR treatment had a significant (P < 0.001 antiproliferative and cytotoxic effect on DU 145 cells. Both effects were time and dose dependent. Also, the dose of gamma-IR which inhibited DNA synthesis and cell proliferation by 50% was 9.7 Gy. Furthermore, gamma-IR induced cell cycle arrest in the G2/M phase and the percentage of cells in the G2/M phase was increased from 15% (control to 49% (IR cells, with a nonsignificant induction of apoptosis. Treatment with 10 Gy gamma-IR for 24, 48, and 72 h stimulated CuZnSOD and MnSOD protein expression in a time-dependent manner, approximately by 3- to 3.5-fold. These data suggest that CuZnSOD and MnSOD enzymes may play an important role in the gamma-IR-induced changes in DU 145 cell growth, cell cycle arrest and cell death.

  9. COL11A1 in FAP polyps and in sporadic colorectal tumors

    Directory of Open Access Journals (Sweden)

    Iselius Lennart

    2001-10-01

    Full Text Available Abstract Background We previously reported that the α-1 chain of type 11 collagen (COL11A1, not normally expressed in the colon, was up-regulated in stromal fibroblasts in most sporadic colorectal carcinomas. Patients with germline mutations in the APC gene show, besides colonic polyposis, symptoms of stromal fibroblast involvement, which could be related to COL11A1 expression. Most colorectal carcinomas are suggested to be a result of an activated Wnt- pathway, most often involving an inactivation of the APC gene or activation of β-catenin. Methods We used normal and polyp tissue samples from one FAP patient and a set of 37 sporadic colorectal carcinomas to find out if the up-regulation of COL11A1 was associated with an active APC/β-catenin pathway. Results In this study we found a statistically significant difference in COL11A1 expression between normal tissue and adenomas from one FAP patient, and all adenomas gave evidence for an active APC/β-catenin pathway. An active Wnt pathway has been suggested to involve stromal expression of WISP-1. We found a strong correlation between WISP-1 and COL11A1 expression in sporadic carcinomas. Conclusions Our results suggest that expression of COL11A1 in colorectal tumors could be associated with the APC/β-catenin pathway in FAP and sporadic colorectal cancer.

  10. La pêcherie du tourteau Cancer pagurus sur les cotes de Bretagne Sud : déplacements, croissance, reproduction

    OpenAIRE

    Le Foll, Anne

    1982-01-01

    La pêche ciblée du crabe tourteau s'est progressivement substituée à celle du homard et des langoustes, surexploités, et depuis quelques années elle constitue une activité importante pour les bateaux de pêche artisanale de divers ports de la Manche et de l'Atlantique. Les chiffres officiels de la Marine Marchande font état d'une production nationale de 10 000 tonnes en 1980. En dépit du caractère récent de cette pêche, des chutes de rendement observées dans certains secteurs ont entraîné des ...

  11. Imaging and PET-CT evaluation of Gi tract cancers; Imagerie et TEP scanner dans les cancers du tube digestif

    Energy Technology Data Exchange (ETDEWEB)

    Laurent, V. [Hopital de Brabois-Vandoeuvre, Service de Radiologie Adultes, 54 - Nancy (France); Olivier, P. [Hopital de Brabois-Vandoeuvre, Service de Medecine Nucleaire, 54 - Nancy (France)

    2008-03-15

    Imaging plays a pivotal role in the management of G.I. tract cancers for diagnosis, characterization, locoregional staging, metastatic work-up and follow-up during and after curative or palliative treatment. The imaging protocols should be optimized and reproducible because of their impact on therapy. Thoracic, abdominal and pelvic CT is the cornerstone of the imaging work-up, optimized and reproducible because of their impact on therapy. Thoracic, abdominal and pelvic CT is the cornerstone of the imaging work-up, optimized and tailored to the specific G.I. segment involved, requiring good G.I. tract distension. Image interpretation of native axial and reformatted multiplanar images is routinely performed. In specific cases, additional targeted imaging with the US or MRI or whole body imaging with PET/CT or MRI may be valuable. PET/CT is a complement to morphological imaging. PET allows detection of lesions otherwise undetected on morphological imaging, usually due to poor contrast with surrounding tissues, and characterization of known lesions. PET/CT is best used as an integral part of a comprehensive imaging work-up. Radiologist and nuclear medicine specialists provide complementary information. each must be familiar with the clinical questions at hand and related stakes, and advantages and limitations of each modality to optimize treatment as part of a multidisciplinary management approach. (authors)

  12. Debate: Pro intraoperative radiation therapy in breast cancer; Debat: pour la radiotherapie peroperatoire dans le cancer du sein

    Energy Technology Data Exchange (ETDEWEB)

    Dubois, J.B.; Lemanski, C.; Azria, D. [Departement de radiotherapie, CRLC Val-d' Aurelle-Paul-Lamarque, 208, rue des Apothicaires, 34298 Montpellier cedex 5 (France); Gutowski, M.; Rouanet, P.; Saint-Aubert, B. [Departement de chirurgie, CRLC Val-d' Aurelle-Paul-Lamarque, 208, rue des Apothicaires, 34298 Montpellier cedex 5 (France)

    2011-10-15

    The use of intraoperative radiation therapy in breast cancer patients started about 20 years ago. Several retrospective and prospective studies have been published. Intraoperative radiation therapy was initially given as a boost to the tumour bed, followed by whole-breast irradiation. These studies have demonstrated the feasibility of the technique, with local control rates and cosmetic results similar to those obtained with standard treatments. Accelerated partial breast irradiation yields local recurrence rates as low as those observed after whole-breast irradiation. Intraoperative radiation therapy as a single irradiation modality with a unique dose has been investigated in recent prospective studies showing satisfactory local results. Intraoperative radiation therapy can be proposed either as a boost or as a unique treatment in selected cases (tumour size, nodal and hormonal status, patient's age). Intraoperative radiation therapy can be delivered by orthovoltage (50 kV) X-rays from mobile generators, or by electrons from linear accelerators, mobile or fixed, dedicated or not to intraoperative radiation therapy. (authors)

  13. Exploring colposcopists' attitudes towards use of HPV testing as a primary screening tool for cervical cancer in British Columbia.

    Science.gov (United States)

    Regier, Dean A; van der Hoek, Kim; Ogilvie, Gina; Smith, Laurie; Henwood, Elena; Miller, Dianne M; McTaggart-Cowan, Helen; Peacock, Stuart J

    2013-07-01

    Objectif : Examiner les attitudes des colposcopistes à l’égard du dépistage de l’ADN du virus du papillome humain (VPH) à titre d’outil principal de dépistage du cancer du col utérin. Méthodes : Des questionnaires administrés en 2010 et en 2011 dans le cadre d’ateliers offerts en Colombie-Britannique se sont penchés sur les attitudes des colposcopistes au moyen de séries d’échelles en cinq points de type Likert (de « fortement en désaccord » à « fortement en accord ») et de questions à réponse binaire (oui / non). La fréquence des réponses « en accord » ou « fortement en accord » a été utilisée pour caractériser les attitudes. Des analyses de régression ont examiné les modifications significatives sur le plan statistique en ce qui concerne les attitudes à la suite de l’atelier de 2010. Résultats : Les réponses ont généralement indiqué des modifications positives en ce qui concerne les attitudes envers le dépistage du VPH. À la suite de l’atelier de 2010, des modifications significatives sur le plan statistique ont été constatées pour ce qui est des articles liés au fait d’être fortement en accord avec la déclaration voulant que le VPH constitue une cause nécessaire du cancer du col utérin (hausse de 39 %; P VPH (hausse de 19 %; P VPH était trop long; en 2011, 53 % ont fait une telle déclaration. Conclusion : Les colposcopistes sont perçus comme étant des leaders d’opinion; ils joueront donc un rôle crucial dans la mise en œuvre du dépistage du VPH en Colombie-Britannique. Notre étude a obtenu des réponses de la part de 73 % (2010) et de 84 % (2011) des colposcopistes inscrits en C.-B. Les colposcopistes étaient en faveur de l’utilisation du dépistage du VPH aux fins du dépistage primaire du cancer du col utérin, mais ne soutenaient pas la mise en œuvre d’un intervalle prolongé dans le cadre du dépistage du VPH, ce qui semble indiquer que la tenue de futurs

  14. Localisation du ganglion sentinelle au moyen de nanoparticules fluorescentes émettant dans le proche infrarouge : Application au cancer du sein

    OpenAIRE

    2012-01-01

    Sentinel lymph node (SLN) biopsy is a reliable technique for the diagnosis of metastases in breast cancer. However, the tracers used (blue dye and radiocolloid) are not optimal because they can cause allergic reactions and major costs in waste processing. Our strategy was to use near-infrared emitting nanoparticles for the mapping of SLN: indium-based Quantum Dots (QDs) and cyanine 7 embedded in silica nanoparticles (SiNP). We investigated indium-based QDs, which are presumed to be less toxic...

  15. Détection précoce du cancer de la prostate chez des apparentés de premier degré au Sénégal

    Directory of Open Access Journals (Sweden)

    B. Fall

    2015-12-01

    Conclusion: Dans cette étude l’association de la race noire et d’un facteur de risque familial ne semblait pas augmenter la fréquence du cancer de la prostate dans notre pays. Cependant d’autres études sur cette question sont nécessaires.

  16. La radiothérapie du cancer de l'endomètre: expérience de l'institut national d'oncologie à propos de 52 cas

    OpenAIRE

    Mezouri, Imane; Berhili, Soufiane; Mouhajir, Nawal; Bellefqih, Sara; Elkacemi, Hanan; Kebdani, Tayeb; Benjaafar, Noureddine

    2016-01-01

    Le cancer de l'endomètre est le cancer gynécologique le plus fréquent en occident. Il concerne principalement les femmes ménopausées. L'objectif de notre travail est de rapporter l'expérience du service de radiothérapie à l'Institut National d'Oncologie (INO) dans la prise en charge du cancer de l'endomètre. Nous avons analysé rétrospectivement 52 cas de cancer de l'endomètre traités dans le service de radiothérapie de l'INO entre 2007 à 2009. Les données collectées à partir des dossiers médi...

  17. “La Constitución de los Pueblos de Occidente”. Une proposition inédite du voyageur cAlî Bey l’Abbasside pour le Maroc dans l´Arxiu Històric de la Ciutat de Barcelona (AHCB, Col. Toda, Ms B.162-II

    Directory of Open Access Journals (Sweden)

    Mercè Viladrich

    2006-11-01

    Full Text Available L’objectif de ce travail est de faire connaître et d’éditer à partir d’un manuscrit autographe le texte intitulé Constitution des peuples d´Occident rédigé par le voyageur catalan Domènec Badía i Leblich (1767-18181, connu sous le nom d’cAlî Bey l’Abbasside. Cette grande charte était destinée à s´implanter comme texte légal dans l´Empire du Maroc. Fonctionnaire au service du premier ministre de Carlos IV, Manuel de Godoy, Domènec Badía voyagea en 1803 au Maroc, dans la péninsule Arabique et au Moyen Orient et gagna une certaine célébrité parmi ses contemporains en racontant ses aventures dans un récit Les voyages d´Ali Bey en Asie et en Afrique, œuvre qui mélange vérité historique et fiction et dans laquelle l´auteur révèle une psychologie pathologique.

  18. Time to surgery and the risk of cancer progression in patients with gynaecologic cancers of the lower genital tract.

    Science.gov (United States)

    Vair, Brett; Altman, Alon D; Nelson, Gregg

    2015-04-01

    Objectif : Nous ne disposons que de peu de données probantes contemporaines pour définir ce qui constitue un délai approprié avant la tenue d’une chirurgie chez des patientes atteintes d’un cancer. Cette étude avait pour but d’examiner si la prolongation du délai avant la tenue d’une chirurgie donne lieu, chez des patientes qui présentent des cancers de stade précoce affectant les voies génitales inférieures, à un risque d’évolution du cancer.  Méthodes : Nous avons mené une étude de cohorte rétrospective en population générale auprès de femmes qui présentaient un carcinome malpighien de stade I affectant le col utérin, la vulve et le vagin, et qui subissaient un traitement chirurgical à Calgary, en Alberta, entre 2000 et 2010. Le stade du cancer et les caractéristiques de la maladie ont été comparées à deux moments distincts : au cours de la consultation initiale auprès du service de gynéco-oncologie et au moment de la chirurgie.  Résultats : Cent cinquante-six patientes ont répondu aux critères d’admissibilité. Une évolution du stade du cancer a été constatée dans 10 cas (6,4 %). Conformément aux cibles actuelles en matière de temps d’attente, le délai avant la tenue d’une chirurgie a été classé comme étant ≤ 28 jours (59 patientes; 38 %) et > 28 jours (97 patientes; 62 %). Une évolution s’est manifestée dans sept des 123 cas de cancer du col utérin (9 %). Dans chacun de ces sept cas, le délai avant la tenue d’une chirurgie avait été > 28 jours (P = 0,095). Aucun des trois cas de cancer du vagin n’a connu une évolution de la maladie. Une évolution s’est manifestée dans trois (10 %) des 30 cas de cancer de la vulve. Dans deux de ces trois cas, le délai avant la tenue d’une chirurgie avait été > 28 jours (P = 0,586). Pour toutes les femmes de la cohorte, le risque relatif d’évolution de la maladie attribuable à la prolongation du délai avant la tenue

  19. Du fratinoj

    Institute of Scientific and Technical Information of China (English)

    1996-01-01

    Vivis iam du fratinoj-Aje kaj Fatme.Iliapatrino estis duon-patrino por Fatme kaj in neamis.Foje i diris al sia edzo:-Faru kion ajn,sed mi ne plu volas vidiFatme.La sekvan tagon la patro forkondukis la

  20. Chemotherapy, brachytherapy and surgery of locally evolved uterine cervix carcinomas: prognosis factors of local control and global survival; Chimioradiotherapie, curietherapie et chirurgie des cancers du col uterin localement evolues: facteurs pronostiques de controle local et de survie globale

    Energy Technology Data Exchange (ETDEWEB)

    Laude, C.; Montella, A.; Montbarbon, X.; Malet, C.; Racadot, S.; Pommier, P. [Centre Leon-Berard, 69 - Lyon (France); Mathevet, P. [Hopital Femme-Mere-Enfant, Hospices Civils de Lyon, 69 - Lyon (France); Buenerd, A. [Centre de Pathologie Est, Hospices Civils de Lyon, 69 - Lyon (France)

    2009-10-15

    The protocol used allows an excellent local control of the uterine cervix carcinoma with an acceptable morbidity. To anticipate the presence of a tumor residue can be an evolution in the therapy management after external radiotherapy, particularly in optimized image-guided brachytherapy (MRI and PET)New utero vaginal applicators with parameters implantation allow to realise the dose complement at the distal parameters. These advances make consider an improvement of results in the management of locally evolved uterine cervix carcinomas. (N.C.)

  1. Extended helical tomo-therapy and concomitant chemotherapy for an uterine cervix carcinoma: dosimetry parameters and hematological toxicity; Tomotherapie helicoidale etendue et chimiotherapie concomitante pour un cancer du col de l'uterus: parametres dosimetriques et toxicite hematologique

    Energy Technology Data Exchange (ETDEWEB)

    Haddad, H.; Thomas, L.; Belhomme, S.; Chemin, A.; Caron, J.; Dejean, C.; Kantor, G.; Richaud, P. [Institut Bergonie, Dept. de Radiotherapie, 33 - Bordeaux (France); Floquet, A.; Guyon, F. [Institut Bergonie, Dept. de Chirurgie, 33 - Bordeaux (France)

    2009-10-15

    the extended tomo-therapy associated to concomitant chemotherapy is feasible and allows a dose escalation at the ganglions level. It is necessary to continue to study the dosimetry parameters at the hematopoietic marrow level that are predictive for a hematological toxicity. (N.C.)

  2. Functional MR Imaging: New tool to predict outcome for cervical carcinoma of uterus treated by concomitant radio chemotherapy?; IRM fonctionnelle: nouvel outil pour predire la reponse des cancers du col uterin a la chimioradiotherapie concomitante?

    Energy Technology Data Exchange (ETDEWEB)

    Taieb, S.; Faivre-Pierret, M.; Ceugnart, L. [Centre Oscar-Lambret, Dept. d' Imagerie, 59 - Lille (France); Nickers, P. [Centre Oscar-Lambret, Dept. de Radiotherapie, 59 - Lille (France); Lesoin, A.; Narducci, F. [Centre Oscar-Lambret, Dept. de Gynecologie Oncologique, 59 - Lille (France)

    2009-10-15

    The treatment of advanced cervix is concomitant radio chemotherapy. Local prognosis and global survival depend on tumoral volume, locoregional extension and radio sensitivity of the lesion. This one is function of tumoral hypoxia, tumoral interstitial pressure and existence of an anaemia. Dynamic contract enhanced MRI (D.C.E.-MRI) allows to quantify pilot vascular parameters of the first two factors. Combined analysis: tumoral volume, anaemia and vascular parameters before and in the course of treatment allows a strong correlation with the risk of local recurrence and global survival. (authors)

  3. Analyse des facteurs histo-pronostiques du cancer du rectum non métastatique dans une série ouest Algérienne de 58 cas au CHU-Tlemcen

    Science.gov (United States)

    Mesli, Smain Nabil; Regagba, Derbali; Tidjane, Anisse; Benkalfat, Mokhtar; Abi-Ayad, Chakib

    2016-01-01

    Introduction L'objectif de notre travail est d'analyser les facteurs histo-pronostiques des cancers du rectum non métastatique opérés au service de chirurgie «A» de Tlemcen à ouest Algérien durant une période de six ans. Méthodes Etude rétrospective de 58 patients qui avait un adénocarcinome rectal. Le critère de jugement était la survie. Les paramètres étudiés, le sexe, l’âge, stade tumoral, et les récidives tumorales. Résultats L’âge moyen était de 58 ans. Avec 52% d'hommes contre 48% femmes avec sex-ratio (1,08). Le siège tumoral était: moyen rectum avec 41,37%, 34,48% au bas rectum et dans 24,13% au haut rectum. La classification TNM avec 17,65% au stade I, 18,61% au stade II, 53, 44% au stade III et 7,84% au stade IV. La survie médiane globale était de 40 mois ±2,937 mois. La survie en fonction du stade tumoral, le stade III et IV avait un faible taux de survie (19%) a 3 ans contre le stade I, II avait un taux de survie de (75%) (P = 0,000) (IC 95%). Les patients avec récidives tumorales avaient un taux de survie faible à 3 ans par rapport à ceux n'ayant pas eu de récidive (30,85% Vs 64,30% P = 0,043). Conclusion Dans cette série, l’étude uni varié des différents facteurs pronostiques conditionnant la survie n'a permis de retenir que trois facteurs influençant la survie, à savoir la taille tumorale, le stade, et les récidives tumorales. En analyse multi variée en utilisant le modèle Cox un seul facteur été retenu la récidive tumorale. PMID:27583069

  4. 龙葵碱对前列腺癌LNCaP及Du145细胞系的作用及机制%Effect and mechanism research of solanine on prostate cancer cell LNCaP and Du145

    Institute of Scientific and Technical Information of China (English)

    李志雄; 梁蔚波; 唐晖; 陈炫; 刘玉峰; 苏泽轩

    2013-01-01

    目的 探讨龙葵碱对雄激素依赖型人前列腺癌LNCaP细胞及雄激素非依赖型人前列腺癌Du145细胞的作用及其机制.方法 应用MTT法检测龙葵碱对两种细胞增殖的影响,流式细胞仪分析龙葵碱对两种细胞细胞周期的影响及细胞凋亡的情况,荧光显微镜原位观察细胞凋亡图像,Western blot法检测龙葵碱对细胞内Bcl-2、Bax、Caspase-3蛋白量表达的影响.结果 龙葵碱能显著抑制两种细胞的增殖,且呈时间与剂量依赖性(P<0.05);能使两种细胞的细胞周期阻滞在S期;各浓度龙葵碱处理组细胞凋亡比例高于0 μg/mL组且荧光显微镜下可见细胞早期凋亡形态图像;龙葵碱能下调两种细胞的Bcl-2蛋白及 Caspase-3蛋白的表达,而保持Bax蛋白的表达不变.结论 龙葵碱可以通过线粒体途径诱导Du145及LNCaP细胞凋亡的发生,能将两种细胞的细胞周期阻滞在S期,产生抑制细胞增殖的作用.%Objective To investigate the role of solanine in human androgen - dependent prostate cancer cell line LNCaP and androgen - independent prostate cancer cell Dul45. Methods The influence on the proliferation of LNCaP and Dul45 cells were evaluated by MTT assay. Cell cycle and cell apoptosis of LNCaP and Dul45 were assessed by flow cytometry and fluorescence microscope. The protein of expressions of Bel -2, Bax, and Caspase -3 were determined by Western blot. Results Solanine could significantly inhibit the proliferation of the two cells in time - and dose - dependent manners (P <0. 05) ; also induced S - phase arrest of LNCaP and Dul45 cells. The apoptosis ratio in treatment groups were significantly higher than that in 0μg/mL group, with early apoptosis. Significant down - regulation of Bel - 2 and Caspase - 3 was induced by solanine, with no effect on Bax protein. Conclusion Solanine provides anti - prostate cancer effect by inhibiting cell proliferation, S -phase arresting, and inducing apoptosis.

  5. Bioenergetic and antiapoptotic properties of mitochondria from cultured human prostate cancer cell lines PC-3, DU145 and LNCaP.

    Directory of Open Access Journals (Sweden)

    Alexander Panov

    Full Text Available The purpose of this work was to reveal the metabolic features of mitochondria that might be essential for inhibition of apoptotic potential in prostate cancer cells. We studied mitochondria isolated from normal prostate epithelial cells (PrEC, metastatic prostate cancer cell lines LNCaP, PC-3, DU145; and non-prostate cancer cells - human fibrosarcoma HT1080 cells; and normal human lymphoblastoid cells. PrEC cells contained 2 to 4 times less mitochondria per gram of cells than the three PC cell lines. Respiratory activities of PrEC cell mitochondria were 5-20-fold lower than PC mitochondria, depending on substrates and the metabolic state, due to lower content and lower activity of the respiratory enzyme complexes. Mitochondria from the three metastatic prostate cancer cell lines revealed several features that are distinctive only to these cells: low affinity of Complex I for NADH, 20-30 mV higher electrical membrane potential (ΔΨ. Unprotected with cyclosporine A (CsA the PC-3 mitochondria required 4 times more Ca²⁺ to open the permeability transition pore (mPTP when compared with the PrEC mitochondria, and they did not undergo swelling even in the presence of alamethicin, a large pore forming antibiotic. In the presence of CsA, the PC-3 mitochondria did not open spontaneously the mPTP. We conclude that the low apoptotic potential of the metastatic PC cells may arise from inhibition of the Ca²⁺-dependent permeability transition due to a very high ΔΨ and higher capacity to sequester Ca²⁺. We suggest that due to the high ΔΨ, mitochondrial metabolism of the metastatic prostate cancer cells is predominantly based on utilization of glutamate and glutamine, which may promote development of cachexia.

  6. Androgen and taxol cause cell type-specific alterations of centrosome and DNA organization in androgen-responsive LNCaP and androgen-independent DU145 prostate cancer cells

    Science.gov (United States)

    Schatten, H.; Ripple, M.; Balczon, R.; Weindruch, R.; Chakrabarti, A.; Taylor, M.; Hueser, C. N.

    2000-01-01

    We investigated the effects of androgen and taxol on the androgen-responsive LNCaP and androgen-independent DU145 prostate cancer cell lines. Cells were treated for 48 and 72 h with 0.05-1 nM of the synthetic androgen R1881 and with 100 nM taxol. Treatment of LNCaP cells with 0.05 nM R1881 led to increased cell proliferation, whereas treatment with 1 nM R1881 resulted in inhibited cell division, DNA cycle arrest, and altered centrosome organization. After treatment with 1 nM R1881, chromatin became clustered, nuclear envelopes convoluted, and mitochondria accumulated around the nucleus. Immunofluorescence microscopy with antibodies to centrosomes showed altered centrosome structure. Although centrosomes were closely associated with the nucleus in untreated cells, they dispersed into the cytoplasm after treatment with 1 nM R1881. Microtubules were only faintly detected in 1 nM R1881-treated LNCaP cells. The effects of taxol included microtubule bundling and altered mitochondria morphology, but not DNA organization. As expected, the androgen-independent prostate cancer cell line DU145 was not affected by R1881. Treatment with taxol resulted in bundling of microtubules in both cell lines. Additional taxol effects were seen in DU145 cells with micronucleation of DNA, an indication of apoptosis. Simultaneous treatment with R1881 and taxol had no additional effects on LNCaP or DU145 cells. These results suggest that LNCaP and DU145 prostate cancer cells show differences not only in androgen responsiveness but in sensitivity to taxol as well. Copyright 2000 Wiley-Liss, Inc.

  7. Col11a1 Regulates Bone Microarchitecture during Embryonic Development

    Directory of Open Access Journals (Sweden)

    Anthony Hafez

    2015-12-01

    Full Text Available Collagen XI alpha 1 (Col11a1 is an extracellular matrix molecule required for embryonic development with a role in both nucleating the formation of fibrils and regulating the diameter of heterotypic fibrils during collagen fibrillar assembly. Although found in many different tissues throughout the vertebrate body, Col11a1 plays an essential role in endochondral ossification. To further understand the function of Col11a1 in the process of bone formation, we compared skeletal mineralization in wild-type (WT mice and Col11a1-deficient mice using X-ray microtomography (micro-CT and histology. Changes in trabecular bone microstructure were observed and are presented here. Additionally, changes to the periosteal bone collar of developing long bones were observed and resulted in an increase in thickness in the case of Col11a1-deficient mice compared to WT littermates. Vertebral bodies were incompletely formed in the absence of Col11a1. The data demonstrate that Col11a1 depletion results in alteration to newly-formed bone and is consistent with a role for Col11a1 in mineralization. These findings indicate that expression of Col11a1 in the growth plate and perichondrium is essential for trabecular bone and bone collar formation during endochondral ossification. The observed changes to mineralized tissues further define the function of Col11a1.

  8. Preoperative radio-chemotherapy for rectal cancer: Forecasting the next steps through ongoing and forthcoming studies; Chimioradiotherapie preoperatoire des cancers du rectum: ce que laissent presager les etudes en cours et a venir

    Energy Technology Data Exchange (ETDEWEB)

    Crehange, G.; Maingon, P. [Departement de radiotherapie, centre Georges-Francois-Leclerc, 1, rue du Professeur-Marion, 21000 Dijon (France); Bosset, J.F. [Service d' oncologie radiotherapie, CHU Jean-Minjoz, boulevard Flemming, 25000 Besancon (France)

    2011-10-15

    Protracted preoperative radio-chemotherapy with a 5-FU-based scheme, or a short course of preoperative radiotherapy without chemotherapy, are the standard neo-adjuvant treatments for resectable stage II-III rectal cancer. Local failure rates are low and reproducible, between 6 and 15% when followed with a 'Total Meso-rectal Excision'. Nevertheless, the therapeutic strategy needs to be improved: distant metastatic recurrence rates remain stable around 30 to 35%, while both sphincter and sexual sequels are still significant. The aim of the present paper was to analyse the ongoing trials listed on the following search engines: the Institut National du Cancer in France, the National Cancer Institute and the National Institute of Health in the United States, and the major cooperative groups. Keywords for the search were: 'rectal cancer', 'preoperative radiotherapy', 'phase II-III', 'preoperative chemotherapy', 'adjuvant chemotherapy' and 'surgery'. Twenty-three trials were selected and classified in different groups, each of them addressing a question of strategy: (1) place of adjuvant chemotherapy; (2) optimization of preoperative radiotherapy; (3) evaluation of new radiosensitization protocols and/or neo-adjuvant chemotherapy; (4) optimization of techniques and timing of surgery; (5) place of radiotherapy for non resectable or metastatic tumors. (authors)

  9. Detection of COL III in Parchment by Amino Acid Analysis

    DEFF Research Database (Denmark)

    Vestergaard Poulsen Sommer, Dorte; Larsen, René

    2016-01-01

    Cultural heritage parchments made from the reticular dermis of animals have been subject to studies of deterioration and conservation by amino acid analysis. The reticular dermis contains a varying mixture of collagen I and III (COL I and III). When dealing with the results of the amino acid...... analyses, till now the COL III content has not been taken into account. Based on the available amino acid sequences we present a method for determining the amount of COL III in the reticular dermis of new and historical parchments calculated from the ratio of Ile/Val. We find COL III contents between 7...... and 32 % in new parchments and between 0.2 and 40 % in the historical parchments. This is consistent with results in the literature. The varying content of COL III has a significant influence on the uncertainty of the amino acid analysis. Although we have not found a simple correlation between the COL...

  10. ATRA inhibits the proliferation of DU145 prostate cancer cells through reducing the methylation level of HOXB13 gene.

    Directory of Open Access Journals (Sweden)

    Zhiwei Liu

    Full Text Available All-trans retinoic acid (ATRA has been widely investigated for treatments of many cancers including prostate cancer. HOXB13, silenced in androgen receptor-negative (AR(- prostate cancer cells, plays a role in AR(- prostate cancer cell growth arrest. In this study we intended to elucidate the mechanisms that are involved in the proliferation inhibition of AR(- prostate cancer cells triggered by ATRA. We discovered that ATRA was able to induce the growth arrest and to increase HOXB13 expression in AR(- prostate cancer cells. Both EZH2 and DNMT3b participated in the repression of HOXB13 expression through an epigenetic mechanism involving DNA and histone methylation modifications. Specifically, EZH2 recruited DNMT3b to HOXB13 promoter to form a repression complex. Moreover, ATRA could upregulate HOXB13 through decreasing EZH2 and DNMT3b expressions and reducing their interactions with the HOXB13 promoter. Concurrently, the methylation level of the HOXB13 promoter was reduced upon the treatment of ATRA. Results from this study implicated a novel effect of ATRA in inhibition of the growth of AR(- resistant human prostate cancer cells through alteration of HOXB13 expression as a result of epigenetic modifications.

  11. Construction of the prostate cancer cell line DU145 with Raptor-shRNA%稳定沉默Raptor表达的前列腺癌DU145细胞株的构建

    Institute of Scientific and Technical Information of China (English)

    潘腾飞; 陈先国; 梁朝朝

    2013-01-01

    目的 应用RNA干扰技术降低前列腺癌DU145细胞株Raptor基因表达水平,并建立稳定转染细胞株.方法 针对Raptor基因设计合成siRNA,鉴定、测序正确后转染293T细胞观察基因表达情况;构建Raptor-shRNA慢病毒载体;进行Raptor-shRNA慢病毒包装及滴度测定;筛选稳定表达Raptor-shRNA的前列腺癌DU145细胞株;Western blot检测稳定转染细胞中的Raptor表达水平.结果 Raptor-shRNA成功转染前列腺癌DU145细胞后,通过嘌呤霉素筛选,获得了Raptor基因沉默的DU145细胞株,Western blot检测证实该细胞株的Raptor表达水平明显低于对照组(P<0.01).结论 成功构建稳定沉默Raptor表达的前列腺癌DU145细胞株,为后续研究奠定了基础.

  12. 体外培养前列腺癌DU145细胞侵袭和迁移能力与EF-1 alpha基因表达的关系%Changes in migration and invasion capability of prostate cancer cell line DU145 after down-regulation of EF-1 alpha gene expression

    Institute of Scientific and Technical Information of China (English)

    朱刚; 钟惟德; 闫伟; 万奔; 王建业

    2009-01-01

    Objective To study the effect of down-rdgulation of EF-1 alpha gene in prostate cancer cell line DU-145 on cancer cell migration and invasion by using RNA interference technique. Methods The prostate cancer cell line DU-145 was divided into three groups: the control group (untransfected with siRNA), randomly control group (randomly transfected with siRNA) and experimental group (transfected with EF-1 alpha siRNA). Localization of EF-1 alpha and its relationship with F-actin in cytoplasm were analyzed by immunofluorescence technique. Cancer cell migration and invasion capability of DU145 cells were studied by transwell technique in these three groups. Results EF-1 alpha expression in DU145 cell line was down-regulated by using RNA interference technique. EF-1 alpha was localized in cytoplasm and co-located with F-actin. The down-regualtion of EF-1 alpha did not change the F-actin distribution in cytoplasm. The cell migration and invasion study showed that after seeding 20×104 DU145 cells into the upper chamber of transwall for 12 hours, the cells collected in the lower chambers were (10.6±1.0)×104 in control group, (11.2±0.8)×104 in randomly control group and (3.9±0.6)×104 in experimental group. Compared with controls, the cancer cell migration and invasion capability was significantly inhibited to only 37.1% (t= 13.9, P<0.05) after the specific down-regulation of EF-1 alpha expression in DU145 cells. Conclusions The down-regulation of EF-1 alpha expression has negative impacts on prostate cancer cell migration and invasion. EF-1 alpha plays important roles in prostate cancer local invasion.%目的 应用RNA干扰技术研究EF-1 alpha基因在体外培养前列腺癌细胞株DU145的侵袭和迁移中的作用. 方法将DU145细胞系分为3组:对照组(未转染siRNA),转染对照组(转染siRNA)和实验组(转染EF-1 alpha-siRNA).通过免疫荧光技术验证RNA干扰结果,并研究EF-1 alpha在DU145细胞中的定位以及与F-actin的定

  13. Id4 Promotes Senescence and Sensitivity to Doxorubicin-induced Apoptosis in DU145 Prostate Cancer Cells

    OpenAIRE

    Carey, Jason P; Knowell, Ashley Evans; Chinaranagari, Swathi; Chaudhary, Jaideep

    2013-01-01

    Inhibitor of differentiation proteins (Id1, 2, 3 and 4) are dominant negative regulators of basic helix loop helix transcription factors and play dominant roles in cancer cells, spanning several molecular pathways including senescence, invasion, metastasis, proliferation and apoptosis. In contrast to high Id1, Id2 and Id3 expression, the expression of Id4 is epigenetically silenced in prostate cancer. In the present study we demonstrated a novel role of Id4, that of promotion of cellular sene...

  14. La maquette digitale du tunnel du LEP

    CERN Document Server

    Brouns, G

    2000-01-01

    Pour l'installation du LHC et son équipement périphérique (ligne cryogénique, câbles, tuyauterie, etc.) dans le tunnel du LEP, une maquette digitale CAO doit être faite. Après un rappel de la définition théorique du tunnel du LEP, cette LHC project Note décrit comment sont utilisées et intégrées d'anciennes et de nouvelles mesures du tunnel pour arriver à un ensemble de données qui permettent de construire la maquette digitale (CAO) du tunnel du LHC. Ensuite, les résultats sont comparés à leur valeur théorique d'une part, et aux anciennes valeurs disponibles d'autre part.

  15. Avaliação da reconstrução do trato alimentar com tubo gástrico ou colônico na esofagectomia por câncer de esôfago Evaluation of the reconstruction techniques of the alimentary tract with gastric or colonic tube in esophagectomy for esophageal cancer

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo Pinto

    2008-12-01

    Full Text Available OBJETIVO: Relatar a experiência com as principais técnicas de reconstrução do trato alimentar após esofagectomia por câncer de esôfago. METODOS: Foram analisados retrospectivamente 68 pacientes submetidos à esofagectomia entre fevereiro de 1997 e novembro de 2005. Todos os pacientes incluídos no estudo foram submetidos à esofagectomia com reconstrução com tubo gástrico ou colônico e anastomose cervical. RESULTADOS: A idade média foi de 55,4 anos (25-74 anos, 50 pacientes eram do sexo masculino e 18 pacientes do sexo feminino, 27 pacientes apresentavam o tumor localizado no esôfago médio e 41 pacientes no esôfago distal, sendo carcinoma epidermóide em 35 pacientes e adenocarcinoma em 33 pacientes. A ressecção foi por via transtorácica em 35 indivíduos e por via transhiatal em 33. A reconstrução com tubo gástrico se deu em 58 pacientes e com tubo colônico em 10 pacientes. A morbidade total da série foi de 52,9%. A mortalidade operatória foi de 5,8%. A sobrevida média foi de 35 meses. CONCLUSÃO: A esofagectomia com reconstrução com tubo gástrico e anastomose cervical é factível tecnicamente, sendo um procedimento realizado de rotina nos pacientes portadores de câncer de esôfago com indicação cirúrgica. Utilizamos, e recomendamos, a reconstrução com tubo colônico principalmente nos pacientes com cirurgia prévia no estômago ou quando da necessidade de ressecção ampliada deste, impossibilitando a confecção da reconstrução do trânsito alimentar com a gastroplastia.BACKGROUND: To describe the main reconstruction techniques of the alimentary tract after esophagectomy due to esophagus cancer in patients enrolled. METHODS: We analyzed retrospectively 68 patients submitted to the esophagectomy between february of 1997 and november of 2005. All of the patients included in the study were submitted to the reconstruction with gastric tube and colon segment and cervical anastomosis. RESULTS: The mean age was

  16. Breast cancer: influence of biochemical and anatomo-pathological factors on the {sup 18}F-F.D.G. fixation;Cancer du sein: influence des facteurs biochimiques et anatomopathologiques sur la fixation du {sup 18}F-FDG

    Energy Technology Data Exchange (ETDEWEB)

    Groheux, D.; Hindie, E.; Vercellino, L.; Moretti, J.L. [Hopital Saint-louis, Service de medecine nucleaire, 75 - Paris (France); Giacchetti, S.; Misset, J.L.; Espie, M. [Hopital Saint-louis, Service des maladies du sein, 75 - Paris (France); Porcher, R. [Hopital Saint-louis, unite de biostatistique, 75 - Paris (France); Lehmann-Che, J. [Hopital Saint-louis, service de biochimie, 75 - Paris (France); De Roquancourt, A. [Hopital Saint-louis, service d' anatomie pathologique, 75 - Paris (France)

    2010-05-15

    The detection of the breast cancer by PET {sup 18}F-F.D.G. depends on the size and nature of the tumor. By disregarding the effect of partial volume, the aim of this work has been to show the impact of biochemical and anatomo-pathological factors on the {sup 18}F-F.D.G. fixation. (N.C.)

  17. col, pepino, lechuga, zanahoria y remolacha

    Directory of Open Access Journals (Sweden)

    Roberto Valdés Roque

    2006-01-01

    Full Text Available El objetivo del presente trabajo fue conformar los complejos tecnológicos para cultivos varios (hortalizas menores: cebolla, ajo, col, pepino, lechuga, zanahoria y remolacha. Para la conformación de los mismos se realizó una revisión bibliográfica relacionada con las temáticas de mecanización, donde se obtuvieron los criterios y orientaciones fundamentales para la selección de los equipos componentes de los complejos tecnológicos, así como las características técnicas que deben tener con el propósito de cumplir las exigencias agrotécnicas de los cultivos. Estos objetivos fueron cumplidos como puede apreciarse en los resultados y las conclusiones.

  18. Metastatic growth from dormant cells induced by a col-I-enriched fibrotic environment.

    Science.gov (United States)

    Barkan, Dalit; El Touny, Lara H; Michalowski, Aleksandra M; Smith, Jane Ann; Chu, Isabel; Davis, Anne Sally; Webster, Joshua D; Hoover, Shelley; Simpson, R Mark; Gauldie, Jack; Green, Jeffrey E

    2010-07-15

    Breast cancer that recurs as metastatic disease many years after primary tumor resection and adjuvant therapy seems to arise from tumor cells that disseminated early in the course of disease but did not develop into clinically apparent lesions. These long-term surviving, disseminated tumor cells maintain a state of dormancy, but may be triggered to proliferate through largely unknown factors. We now show that the induction of fibrosis, associated with deposition of type I collagen (Col-I) in the in vivo metastatic microenvironment, induces dormant D2.0R cells to form proliferative metastatic lesions through beta1-integrin signaling. In vitro studies using a three-dimensional culture system modeling dormancy showed that Col-I induces quiescent D2.0R cells to proliferate through beta1-integrin activation of SRC and focal adhesion kinase, leading to extracellular signal-regulated kinase (ERK)-dependent myosin light chain phosphorylation by myosin light chain kinase and actin stress fiber formation. Blocking beta1-integrin, Src, ERK, or myosin light chain kinase by short hairpin RNA or pharmacologic approaches inhibited Col-I-induced activation of this signaling cascade, cytoskeletal reorganization, and proliferation. These findings show that fibrosis with Col-I enrichment at the metastatic site may be a critical determinant of cytoskeletal reorganization in dormant tumor cells, leading to their transition from dormancy to metastatic growth. Thus, inhibiting Col-I production, its interaction with beta1-integrin, and downstream signaling of beta1-integrin may be important strategies for preventing or treating recurrent metastatic disease.

  19. Pacemaker, implanted cardiac defibrillator and irradiation: Management proposal in 2010 depending on the type of cardiac stimulator and prognosis and location of cancer; Pacemaker, defibrillateur et radiotherapie: propositions de conduite a tenir en 2010 en fonction du type de stimulateur cardiaque, du pronostic et du site du cancer

    Energy Technology Data Exchange (ETDEWEB)

    Lambert, P. [Service d' anesthesie reanimation, hopital Nord, centre hospitalier universitaire de Saint-etienne, 42055 Saint-etienne cedex 2 (France); Da Costa, A. [Service de cardiologie, hopital Nord, centre hospitalier universitaire de Saint-etienne, 42055 Saint-etienne cedex 2 (France); Marcy, P.Y. [Departement de radiologie, centre Antoine-Lacassagne, 33, avenue de Valombrose, 06189 Nice cedex 2 (France); Universite Nice Sophia-Antipolis, 33, avenue de Valombrose, 06189 Nice cedex 2 (France); Kreps, S. [Service de radiotherapie Corad, centre regional universitaire de cancerologie Henry-S.-Kaplan, hopital Bretonneau-2, CHU de Tours, boulevard Tonnelle, 37000 Tours (France); Angellier, G.; Marcie, S.; Bondiau, P.Y. [Universite Nice Sophia-Antipolis, 33, avenue de Valombrose, 06189 Nice cedex 2 (France); Departement de radiotherapie oncologie, centre CyberKnife, centre Antoine-Lacassagne, 33, avenue de Valombrose, 06189 Nice cedex 2 (France); Briand-Amoros, C. [Service de radiotherapie, hopital europeen Georges-Pompidou, 20, rue Leblanc, 75015 Paris (France); Thariat, J. [Universite Nice Sophia-Antipolis, 33, avenue de Valombrose, 06189 Nice cedex 2 (France); Departement de radiotherapie oncologie, centre CyberKnife, centre Antoine-Lacassagne, 33, avenue de Valombrose, 06189 Nice cedex 2 (France); IBDC CNRS UMR 6543, centre Antoine-Lacassagne, universite Sophia-Antipolis, 33, avenue de Valombrose, 06189 Nice cedex 2 (France)

    2011-06-15

    Ionizing radiation may interfere with electric components of pacemakers or implantable cardioverter defibrillators. The type, severity and extent of radiation damage to pacemakers, have previously been shown to depend on the total dose and dose rate. Over 300,000 new cancer cases are treated yearly in France, among which 60% are irradiated in the course of their disease. One among 400 of these patients has an implanted pacemaker or defibrillator. The incidence of pacemaker and implanted cardioverter defibrillator increases in an ageing population. The oncologic prognosis must be weighted against the cardiologic prognosis in a multidisciplinary and transversal setting. Innovative irradiation techniques and technological sophistications of pacemakers and implantable cardioverter-defibrillators (with the introduction of more radiosensitive complementary metal-oxide-semiconductors since 1970) have potentially changed the tolerance profiles. This review of the literature studied the geometric, dosimetric and radiobiological characteristics of the radiation beams for high energy photons, stereotactic irradiation, proton-therapy. Standardized protocols and radiotherapy optimization (particle, treatment fields, energy) are advisable in order to improve patient management during radiotherapy and prolonged monitoring is necessary following radiation therapy. The dose received at the pacemaker/heart should be calculated. The threshold for the cumulated dose to the pacemaker/implantable cardioverter-defibrillator (2 to 5 Gy depending on the brand), the necessity to remove/displace the device based on the dose-volume histogram on dosimetry, as well as the use of lead shielding and magnet are discussed. (authors)

  20. D(E)TECTION DE LA MUTATION DU G(E)NE HMLH1 DANS LE CANCER COLO-RECTAL PAR(E)LECTROPHOR(E)SE CAPILAILRE ASSOCI(E)E (A) LA FLUORESCENCE LASER-INDUITE

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Objectif L'objectif de ce travail est d'etablir une méthode d'analyse du polymorphisme de la conformation monochaine ( PCMC), capable de détecter la mutation ponctuelle du gène hMLH1 dans le cancer colique en utilisant l' l ctrophor se capillaire associée à la fluorescence laser-induite (FLI-EC). Méthodes Les exons 12 du gène hMLH1 du sang périphérique de 42 patients de cancer colo-rectal sporadique et 20 sujets sains témoins sont amplifiés par la PCR. Le PCMC des produits du PCR est analysé par FLI-EC. Les échantillons anormaux ont été confirmés par séquen age EC. Les effets de la concentration du milieu ( LPA ) , de la température de séparationcet du voltage de séparation sur le comportement de EC ont été aussi etudiés. Résultats L' analyse de PCMC par la methode FLI-EC a retrouvé une mutation hétérozygote chez 4 des 42 patients. La mutation ponctuelle de T1l51A a été objectivée par la séquen age EC de ces échantillons, aucune mutation n'a été retrouvée chez les 20 sujets sains témoins. La séparation des pics d'ADN monochainaux a été facilitée par une légère augmentation de la concentration de LPA (4%-6% ), une légère baisse de la température de séparation ( 20℃) et une légère élévation du voltage de séparation ( 9kV). Conclusion Une concentration adéquate de LPA, une température de séparation appropriée et un voltage de séparation bien approprié améliorent considérablement l'efficacité du FLI-EC.L'application de cette méthode pour détecter la mutation ponctuelle du gène hMLH1 est d'une rapidité, d'une efficacité, et d'uneéreproductivit consid rables. Cette méthode de réalisation facile reste très prometteur pour le dépistage rapide et massive des mutations génétiques.

  1. Which techniques for an additional irradiation of the tumour bed in a breast cancer?; Quelles techniques de complement d'irradiation du lit tumoral dans le cancer du sein?

    Energy Technology Data Exchange (ETDEWEB)

    Chenna, H.; Iraqi, M.; Ahmedou, M.M.; Berhil, H.; El Kacemi, H.; Hassouni, K.; Kebdani, T.; Benjaafar, N.; El Gueddari, B.K. [Service de radiotherapie, Institut national d' oncologie, Rabat (Morocco)

    2010-10-15

    The authors report a comparison of different techniques for an additional irradiation of the tumour bed, in terms of local control and aesthetic result in the case of a breast cancer. This additional irradiation has been delivered by electron beam in five fractions, high dose rate curie-therapy in two fractions, photon beam in five or six fractions, and low dose rate curie-therapy. The dose increase in the tumour bed allows the local control rate to be increased without compromising aesthetic results. However, the comparison of the different boost techniques does not reveal significant differences. Short communication

  2. Rare hereditary COL4A3/COL4A4 variants may be mistaken for familial focal segmental glomerulosclerosis

    Science.gov (United States)

    Malone, Andrew F; Phelan, Paul J; Hall, Gentzon; Cetincelik, Umran; Homstad, Alison; Alonso, Andrea; Jiang, Ruiji; Lindsey, Thomas; Wu, Guanghong; Sparks, Matthew A; Smith, Stephen R; Webb, Nicholas J A; Kalra, Philip; Adeyemo, Adebowale; Shaw, Andrey S; Conlon, Peter J; Jennette, J Charles; Howell, David N; Winn, Michelle P; Gbadegesin, Rasheed A

    2014-01-01

    Focal segmental glomerulosclerosis (FSGS) is a histological lesion with many causes including inherited genetic defects with significant proteinuria being the predominant clinical finding at presentation. Mutations in COL4A3 and COL4A4 are known to cause Alport syndrome, thin basement membrane nephropathy, and to result in pathognomonic glomerular basement membrane findings. Secondary FSGS is known to develop in classic Alport Syndrome at later stages of the disease. Here, we present seven families with rare or novel variants in COL4A3 or COL4A4 (six with single and one with two heterozygous variants) from a cohort of 70 families with a diagnosis of hereditary FSGS. The predominant clinical findings at diagnosis were proteinuria associated with hematuria. In all seven families, there were individuals with nephrotic range proteinuria with histologic features of FSGS by light microscopy. In one family, electron microscopy showed thin glomerular basement membrane, but four other families had variable findings inconsistent with classical Alport nephritis. There was no recurrence of disease after kidney transplantation. Families with COL4A3 and COL4A4 variants that segregated with disease represent 10% of our cohort. Thus, COL4A3 and COL4A4 variants should be considered in the interpretation of next-generation sequencing data from such patients. Furthermore, this study illustrates the power of molecular genetic diagnostics in the clarification of renal phenotypes. PMID:25229338

  3. Thérapies lasers focales du cancer de prostate guidées par l'image

    OpenAIRE

    Colin, Pierre

    2012-01-01

    Given the increased incidence of prostate cancer low-risk, focal therapies appear as an attractive alternative between active surveillance and radical treatments. Laser therapies (photodynamic therapy [PDT] and interstitial laser thermotherapy [LITT]) could be modalities of application of this focal therapy.In the first section of this thesis, we discuss the concept of focal therapy, and the place of laser therapies compared to several other energy modalities. The first chapter of this sectio...

  4. La longévité du pollen de colza

    OpenAIRE

    Pierre Jacqueline; Renard Michel

    2002-01-01

    Connaître la longévité d’un pollen est une donnée particulièrement intéressante dans le cadre des études de risques de dissémination du pollen. En effet, cela permet de savoir combien de temps une parcelle dont la floraison est terminée reste une source de pollen fécondant. Par ailleurs, une étude de la longévité du pollen pourrait permettre de mieux comprendre les résultats en apparence contradictoires obtenus au cours de diverses expérimentations menées sur la dissémination du pollen de col...

  5. Local recurrences after breast-conserving treatments in breast cancer: risk factors and effect on survival; Recidives locales apres traitement conservateur du cancer du sein: facteurs de risque et influence sur la survie

    Energy Technology Data Exchange (ETDEWEB)

    Cowen, D.; Resbeut, M.; Largillier, R.; Houvenaeghel, G.; Jacquemier, J.; Bardou, V.J.; Viens, P.; Maraninchi, D. [Institut Paoli-Calmettes, 13 - Marseille (France)

    1998-09-01

    To determine the risk factors for local and distant failure in node-negative breast cancer treated with breast-conservative surgery and radiotherapy and to determine the relationship between these two events. We retrospectively selected 908 patients who received conservative surgery and radiotherapy but no chemotherapy between 1980 and 1995, for a mode-negative breast cancer. Patients were divided in two groups according to the status of the margins of resection. All pathology specimens were reviewed. In case of negative margins, the risk factors for local recurrences picked up by the Cox model were histologic multi-focus (P=0.0076), peritumoral vessel invasion (P=0.021) and age {>=}40 years (P=0.024), and in case of involved margins, negative oestrogen receptors (P=0.0012), histologic multi-focus (P=0.0028), and absence of hormonal therapy (P=0.017). The 10-year local recurrence rate was 18 % in case of negative margins and 29 % in case of involved margins, although in the latter case patients received high-dose adjuvant radiotherapy. Accordingly, the 10-year distant failure rates were 16 % and 27 %, respectively. Many arguments suggest that local and distant failures are closely related. Patients with histologic multi-focus or positive margins are at high risk of local failure and then of distant failure, and require a more aggressive initial treatment. (author)

  6. The role of brachytherapy in the definitive management of prostate cancer; Place de la curietherapie dans le traitement du cancer prostatique localise

    Energy Technology Data Exchange (ETDEWEB)

    Crook, J. [British Columbia Cancer Agency, Center for the Southern Interior, 399, Royal Avenue, Kelowna, British Columbia, V1Y 5L33 (Canada)

    2011-06-15

    Over the past two decades, brachytherapy has played an ever expanding role in the definitive radiotherapy of prostate cancer. Brachytherapy surpasses external beam radiotherapy in its ability to deliver intense intra-prostatic dose escalation. Although initially low dose rate permanent seed brachytherapy was favored for favorable risk prostate cancers, and high dose rate temporary brachytherapy for intermediate and advanced disease, both types of brachytherapy now have a place across all the risk groups of localized prostate cancer. This article will review indications and patient selection, planning and technical aspects, toxicity and efficacy for both low and high dose rate prostate brachytherapy. (author)

  7. The future of breast cancer radiotherapy: From one size fits all to taylor-made treatment; L'avenir de la radiotherapie du cancer du sein: de la taille unique au sur-mesure

    Energy Technology Data Exchange (ETDEWEB)

    Hennequin, C. [Service de cancerologie-radiotherapie, hopital Saint-Louis, 1, avenue Claude-Vellefaux, 75475 Paris (France); Azria, D. [Departement de cancerologie radiotherapie, CRLC Val-d' Aurelle-Paul-Lamarque, rue Croix-Verte, 34298 Montpellier cedex 5 (France); Universite de Montpellier I, 5, boulevard Henri-IV, CS 19044, 34967 Montpellier cedex 2 (France); Inserm U896, institut de recherche en cancerologie de Montpellier, CRLC Val-d' Aurelle-Paul-Lamarque, rue Croix-Verte, 34298 Montpellier cedex 5 (France)

    2011-10-15

    Various subgroups of breast tumours have been identified during the last 10 years according to the risk of local relapse. Prognostic factors for local relapse are age, surgical margins, tumour size, Her2 expression and hormonal receptors status. For tumours with a high risk of local relapse, an increased in boost dose or the addition of new drugs (trastuzumab, anti-angiogenics, PARP inhibitors) could be considered. For low risk tumours, hypo-fractionated, accelerated partial breast and intraoperative radiotherapy are being evaluated. The classical schedule (45-50 Gy to the whole gland followed by a boost dose of 16 Gy) is no longer the universal rule. Treatment individualization, according to clinical and biological characteristics of the tumour and - possibly - to the radiobiological profile of the patient, is likely to be the future of breast cancer radiotherapy. (authors)

  8. Surface hyperthermia guided by infrared image and re-irradiation of local relapses of breast cancer; Hyperthermie superficielle guidee par image infrarouge et la re-irradiation des recidives locales du cancer du sein

    Energy Technology Data Exchange (ETDEWEB)

    Notter, M.; Yanes, B.; Germond, J.F. [Hopital Neuchatelois, La Chaux de Fonds (Switzerland)

    2010-10-15

    As the taking into care of local relapses of cancer breast after a conventional treatment remains a challenge, the authors report the exploration of the use of a re-irradiation combined with hyperthermia. As disappointing results obtained with surface hyperthermia are attributed to some technical problems, they use a high resolution infrared image to get a better temperature distribution and to be able to adapt heat application to the dynamic changes of the tumour and of normal tissues, and to improve the hyperthermia quality. Preliminary results obtained on five patients are discussed and seem promising. It provides a better therapeutic efficiency, and gives access to information related to the tumour response, to inflammatory processes, to the vascularisation of healthy tissues, and to radio-induced reactions. Short communication

  9. Prone decubitus dual incidence dynamic lymphoscintigraphy for sentinel lymph node localization in breast cancer; Lymphoscintigraphie dynamique en decubitus ventral et double incidence pour la localisation du ganglion sentinelle des cancers du sein

    Energy Technology Data Exchange (ETDEWEB)

    Gremillet, E.; Soler, C.; Champailler, A.; Griot, A.; Berger, E.; Griot, J.P.; Villard, P.; Bouteille, C. [Centre Hospitalier Prive de la Loire, 42 - Saint-Etienne (France)

    2000-09-01

    The sentinel lymph mode (SLN) technique in breast cancer is aimed to avoid useless axillary lymph node dissection (ALND). We present here our experience in a series of 42 patients. After some protocol evolutions imposed by the first results, 37 patients were similarly studied: 4 to 6 peritumoral injections (0,75 ml sulfur colloid labeled with {sup 99m}Tc) were immediately followed by local gentle massage for 5 min, and then by dynamic imaging (anterior and lateral simultaneously) in prone decubitus with pending breast. Then the SLN was skin-land-marked in surgical position (dorsal decubitus) in two incidences (typically anterior and lateral); SLN localisation on ventral decubitus images directed additional actions as necessary: pulling the breast, oblique incidence. Upon surgery, after primary lesion malignancy confirmation, the SLN was electively dissected and then the usual ALND was performed. If we exclude from analysis the 4 cases of previous lumpectomy (among which we had one total lack of tracer migration and one prediction error), the final diagnostic results were perfect: lympho-scintigraphic and per-operative SLN detection were 100%, predictive error was 0%. This result seems to be mainly due to prone decubitus imaging which moves away peritumoral activity from axilla and thus makes easier final land-marking in dorsal decubitus. (author)

  10. La recherche de la surexpression de la protéine et l'amplification du gène HER2 dans le cancer de l'estomac par immunohistochimie et Hybridation in Situ: expérience du CHU HASSAN II de Fès

    OpenAIRE

    El Fatemi, Hinde; Hammas, Nawal; Idrissi, Karima; Mellas, Nawfel; Bennani, Amal; Amarti, Afaf; Cayre, Anne; Penault-Llorca, Frederique; Mesbahi, Omar

    2012-01-01

    La surexpression de l'Her2 a été détectée dans plusieurs cancers et a été particulièrement étudiée dans le cancer du sein. Elle est décrite dans 10 à 30% des adénocarcinomes gastriques. Un statut HER2 positif est un facteur de mauvais pronostic et un facteur prédictif de la réponse à l'herceptine (trastuzumab). Cet article présente les résultats d'une étude préliminaire portant sur 31 cas de tumeurs gastriques, et dont le but est d’évaluer la surexpression de l’ HER2 dans les adénocarcinomes ...

  11. Epidémiologie du cancer gastrique: expérience d'un centre hospitalier marocain

    OpenAIRE

    Mellouki, Ihsane; laazar, Nawal; Benyachou, Bahija; Aqodad, Nouredine; Ibrahimi, Adil

    2014-01-01

    Le cancer de l'estomac est représenté essentiellement par Les adénocarcinomes gastriques, ces derniers demeurent l'une des dix premières causes mondiales de mortalité avec un pronostic qui est péjoratif. Son incidence reste variable à travers le monde, elle est caractérisée par une importante disparité géographique. Le but de notre travail est de décrire les caractéristiques épidémiologiques de l'adénocarcinome gastrique dans notre contexte à travers une étude rétrospective, observationnelle ...

  12. SearchCol: Metaheuristic Search by Column Generation

    Science.gov (United States)

    Alvelos, Filipe; de Sousa, Amaro; Santos, Dorabella

    We propose a general framework for the combination of column generation (CG) with metaheuristics (MHs) aiming to solve combinatorial optimization problems amenable for decomposition approaches, "SearchCol". The combination of the two approaches is based on representing the solution of the problem as the selection of a subset of solutions of smaller (sub)problems which are generated by CG. In each iteration of a SearchCol algorithm, CG provides the optimal solution (primal and dual) to the MH which returns a (incumbent) solution used for defining the (perturbed) CG problem of the next iteration. We describe the SearchCol framework and a general SearchCol algorithm based on local search.

  13. Hypo-fractionated whole breast irradiation: Pro and cons; Irradiation hypofractionnee dans le cancer du sein: pour ou contre?

    Energy Technology Data Exchange (ETDEWEB)

    Cutuli, B. [Institut du cancer Courlancy, 38, rue de Courlancy, 51100 Reims (France); Fourquet, A. [Institut Curie, 26, rue d' Ulm, 75005 Paris (France)

    2011-10-15

    The continuous increase of breast cancer (BC) incidence, the logistic constraints of the protracted standard 5-week radiations regimen have led to test short hypo-fractionated whole breast radiation therapy schemes. Three prospective randomized trials and a pilot trial have been published. Large numbers of patients were included, with follow-up duration ranging from 5 to 12 years. The conclusions of these trials were similar, showing local control and toxicity equivalent to those of the standard regimen, and supporting the use of three schemes: 42.5 Gy/16 fractions/3 weeks, 40 Gy/15 fractions/3 weeks or 41.6 Gy/13 fractions/5 weeks. However, the patients in these trials had favourable prognostic factors, were treated to the breast only and the boost dose, when indicated, was delivered with a standard fractionation. Hypo-fractionated treatment can only be recommended in patients treated to the breast only, without nodal involvement, with grade < 3 tumours and who are not candidate to chemotherapy. If a boost is to be given, a standard fractionation should be used. Particular care should be taken to avoid heterogeneities leading to high fractional doses to organs at risk (lung and heart). (authors)

  14. Detection of COL III in parchment by amino acid analysis.

    Science.gov (United States)

    Sommer, Dorte V P; Larsen, René

    2016-01-01

    Cultural heritage parchments made from the reticular dermis of animals have been subject to studies of deterioration and conservation by amino acid analysis. The reticular dermis contains a varying mixture of collagen I and III (COL I and III). When dealing with the results of the amino acid analyses, till now the COL III content has not been taken into account. Based on the available amino acid sequences, we present a method for determining the amount of COL III in the reticular dermis of new and historical parchments calculated from the ratio of Ile/Val. We find COL III contents between 7 and 32 % in new parchments and between 0.2 and 40 % in the historical parchments. This is consistent with results in the literature. The varying content of COL III has a significant influence on the uncertainty of the amino acid analysis. Although we have not found a simple correlation between the COL III content and the degree of deterioration, our results show that this question must be taken into consideration in future studies of the chemical and physical deterioration of parchment measured by amino acid analysis and other analytical methods.

  15. Rare hereditary COL4A3/COL4A4 variants may be mistaken for familial focal segmental glomerulosclerosis

    OpenAIRE

    Malone, Andrew F.; Phelan, Paul J.; Hall, Gentzon; Cetincelik, Umran; Homstad, Alison; Alonso, Andrea; Jiang, Ruiji; Lindsey, Thomas; Wu, Guanghong; Sparks, Matthew A.; Smith, Stephen R.; Webb, Nicholas J. A.; Kalra, Philip; Adeyemo, Adebowale; Shaw, Andrey S.

    2014-01-01

    Focal segmental glomerulosclerosis (FSGS) is a histological lesion with many causes including inherited genetic defects with significant proteinuria being the predominant clinical finding at presentation. Mutations in COL4A3 and COL4A4 are known to cause Alport syndrome, thin basement membrane nephropathy, and to result in pathognomonic glomerular basement membrane findings. Secondary FSGS is known to develop in classic Alport Syndrome at later stages of the disease. Here, we present seven fa...

  16. Cancer

    Science.gov (United States)

    ... cancer Non-Hodgkin lymphoma Ovarian cancer Pancreatic cancer Testicular cancer Thyroid cancer Uterine cancer Symptoms Symptoms of cancer ... tumor Obesity Pancreatic cancer Prostate cancer Stomach cancer Testicular cancer Throat or larynx cancer Thyroid cancer Patient Instructions ...

  17. Association synchrone d'un cancer du sein bilatéral et d'une tumeur stromale gastro-intestinale: à propos d'un rare cas

    OpenAIRE

    Afif, Mohammed; Ouziane, Imane; Kouhen, Fadila; Khalil, Jihane; Elomrani, Fedwa; Elkacemi, Hanan; Kebdani, Tayeb; Errihani, Hassan; Benjaafar, Noureddine

    2015-01-01

    Les tumeurs stromales gastro-intestinales sont les tumeurs mésenchymateuses les plus fréquentes, pourtant, leur association avec les tumeurs du sein sont rares, seulement quelques cas cliniques sont rapportés dans la littérature. Nous rapportons l'observation d'une jeune femme de 41 ans, suivie à l'institut national d'oncologie de Rabat, pour un carcinome canalaire du sein, bilatéral, chez qui le bilan d'extension a objectivé une tumeur stromale de type gastro-intestinale aux dépens de l'esto...

  18. Teeth and irradiation in head and neck cancer; Dent et irradiation: denture et consequences sur la denture de la radiotherapie des cancers de la tete et du cou

    Energy Technology Data Exchange (ETDEWEB)

    Thariat, J.; Ortholan, C. [Centre Antoine-Lacassagne, Service de radiotherapie, 06 - Nice (France); Thariat, J.; Darcourt, V.; Poissonnet, G.; Dassonville, O.; Marcy, P.Y.; Bozec, A.; Ortholan, C.; Santini, J. [Universite de Nice Sophia-Antipolis, 06 - Nice (France); Thariat, J. [IBDC CNRS UMR 6543, 06 - Nice (France); Mones, E. de [CHU de Bordeaux, Service ORL, 33 - Bordeaux (France); Darcourt, V. [Centre Antoine-Lacassagne, Service de radiotherapie-dentisterie, 06 - Nice (France); Poissonnet, G.; Dassonville, O.; Bozec, A.; Santini, J. [Centre Antoine-Lacassagne, Service ORL, 06 - Nice (France); Savoldelli, C.; Odin, G.; Guevara, N. [CHU, Service ORL et de chirurgie maxillofaciale, 06 - Nice (France); Marcy, P.Y. [Centre Antoine-Lacassagne, Service de radiologie, 06 - Nice (France); Bensadoun, R.J. [CHU, Service de radiotherapie, 86 - Poitiers (France)

    2010-04-15

    Pre-irradiation dental care depends on teeth health, fields and dose of irradiation, compliance to fluorides, cessation of tobacco and psycho-social cofactors. Dental care aims at preventing complications and preserving the quality of life (eating, speech and aesthetics). Approximately 11% of patients do not require any pre-irradiation dental care. Dental complications vary from slight colorations of the teeth to major complication such as osteoradionecrosis. Osteoradionecrosis rates vary from 1 to 9%, and may be decreased by using a 21-day delay between extractions and irradiation, provided that it does not postpone cancer treatment, with a dose-dependent risk (<6% if <40 Gy; 14% between 40 et 60 Gy; =20% if >60 Gy). Osteoradionecrosis occurs spontaneously (35%), mostly involves the mandible (85%). (authors)

  19. Internal mammary chain irradiation in breast cancer: State of the art; Radiotherapie de la chaine mammaire interne dans les cancers du sein: etat des lieux

    Energy Technology Data Exchange (ETDEWEB)

    Auberdiac, P.; Cartier, L.; Hau Desbat, N.H.; De Laroche, G.; Magne, N. [Unite de curietherapie, departement de radiotherapie, institut de cancerologie de la Loire, 108 bis, avenue Albert-Raimond, BP 60008, 42271 Saint-Priest-en-Jarez cedex (France); Chargari, C. [Service d' oncologie radiotherapie, hopital d' instruction des armees du Val-de-Grace, 74, boulevard Port-Royal, 75230 Paris cedex 5 (France); Zioueche, A. [Service de radiotherapie, CHU Dupuytren, 2, avenue Martin-Luther-King, 87000 Limoges (France); Melis, A. [Departement d' oncologie medicale, institut de cancerologie de la Loire, 108 bis, avenue Albert-Raimond, 42271 Saint-Priest-en-Jarez cedex (France); Kirova, Y.M. [Service de radiotherapie oncologique, institut Curie, 26, rue d' Ulm, 75005 Paris (France)

    2011-04-15

    Radiation therapy has a major role in the management of infiltrative breast cancers. However, there is no consensus for the prophylactic treatment of the internal mammary chain (IMC), with strategies that show strong differences according to centers and physicians. Indications for internal mammary chain radiotherapy are debated, since this treatment significantly increases the dose delivered to the heart and leads to potential technical difficulties. Important prospective data recently suggested that internal mammary chain radiotherapy would not be necessary, even in cases of internal or central tumor locations, or in patients with positive axillary lymph nodes. Although these data warrant confirmation by two other prospective trials, there is evidence that the indications for internal mammary chain radiotherapy should be careful and that high quality techniques should be used for decreasing the dose delivered to the heart. This review of literature presents the state of art on the radiotherapy of internal mammary chain, with special focus on the indications, techniques, and potential toxicity. (authors)

  20. "Cirque du Freak."

    Science.gov (United States)

    Rivett, Miriam

    2002-01-01

    Considers the marketing strategies that underpin the success of the "Cirque du Freak" series. Describes how "Cirque du Freak" is an account of events in the life of schoolboy Darren Shan. Notes that it is another reworking of the vampire narrative, a sub-genre of horror writing that has proved highly popular with both adult and…

  1. Mapeig col·laboratiu de conflictes socioambientals

    OpenAIRE

    Pérez López, Alfons

    2011-01-01

    L’objectiu general del MCA és desenvolupar una eina que permeti, per una banda, representar i publicar informació en base cartogràfica de conflictes socioambientals per fer-los visibles i despertar la consciència pública, enfortint i apoderant els afectats i afectades, organitzacions socials que els/les hi donen suport; i d’altra banda, que permeti treballar conjuntament i de manera col·laborativa, la informació dels conflictes socioambientals. Els objectius específics del mapatge col·l...

  2. Restaurant du Rivage, Vevey

    OpenAIRE

    Basini, Sari Bianca; Glocki, Ryszard Nikodem

    2015-01-01

    Après cinquante ans de mutilations, d'abandon et de spéculations économiques, le complexe du château de l'Aile et de la salle du Castillo à Vevey doit redéfinir son rôle public par rapport à la place du Marché et au Jardin du Rivage. S'appuyant sur la mémoire historique en ajoutant une unité à l'ensemble, nous créons un îlot regroupant des fonctions publiques. Il dessert ainsi l'espace ouvert environnant en articulant la relation entre le jardin et la place. L'élargissement de la promenade du...

  3. Dosimetric comparison of helical tomo-therapy and standard three-dimensional conformations radiotherapy within the frame of the treatment of a left breast cancer; Comparaison dosimetrique d'une tomotherapie helicoidale et d'une radiotherapie standard conformationnelle tridimensionnelle dans le cadre du traitement du cancer du sein gauche

    Energy Technology Data Exchange (ETDEWEB)

    Abrous-Anane, S.; Dessard-Diana, B.; Durdux, C.; Giraud, P.; Housset, M.; Giraud, P. [Hopital europeen Georges-Pompidou, 75 - Paris (France); Yassa, M. [Hopital Maisonneuve-Rosemont, Montreal (Canada); Hijal, T. [McGill University Health Centre, Montreal (Canada)

    2010-10-15

    The use of intensity-modulated conformational radiotherapy (IMRT) resulted in a reduction of early side effects for patients treated by total mammary irradiation. Helical tomo-therapy uses a great number of circumferential beams which are modulated by an ultra-fast multi-blade collimator to deliver an IMRT with a high dose gradient. However, the use of this technique has been limited for the treatment of breast cancer because of the increased treatment duration and of the increase of low doses for organs at risk. The authors describe a simple technique to irradiate in a conformational and homogeneous way the whole mammary target volume while reducing the dose delivered to organs at risk. They discuss the results obtained during eight treatments of patients suffering from a left breast cancer. Short communication

  4. Complete COL1A1 allele deletions in osteogenesis imperfecta

    NARCIS (Netherlands)

    van Dijk, Fleur S.; Huizer, Margriet; Kariminejad, Ariana; Marcelis, Carlo L.; Plomp, Astrid S.; Terhal, Paulien A.; Meijers-Heijboer, Hanne; Weiss, Marjan M.; van Rijn, Rick R.; Cobben, Jan M.; Pals, Gerard

    2010-01-01

    Purpose: To identify a molecular genetic cause in patients with a clinical diagnosis of osteogenesis imperfecta (OI) type I/IV. Methods: The authors performed multiplex ligation-dependent probe amplification analysis of the COL1A1 gene in a group of 106 index patients. Results: In four families with

  5. EuroCirCol: A key to New Physics

    CERN Multimedia

    Johannes Gutleber

    2015-01-01

    Monday 1 June saw the start of EuroCirCol, the EC-funded part of the FCC study that will develop the conceptual design for an energy-frontier hadron collider.   Attendees at the EuroCirCol meeting at CERN. The EuroCirCol kick-off event at CERN on 2 to 4 June brought together 62 participants to constitute governance bodies, commit to the project plan and align the organisation, structures and processes of 16 institutions from 10 countries. The goal of the project is to conceive a post-LHC research infrastructure around a 100 km circular energy-frontier hadron collider capable of reaching 100 TeV collisions. The project officially started on 1 June and will run for four years. The total estimated budget of 11.2 MEUR includes a 2.99 MEUR contribution from the Horizon 2020 programme dedicated to the development of new world-class research infrastructures. EuroCirCol will deliver a design for a hadron collider as part of the broader Future Circular Collider (FCC) study. It will provide input to an...

  6. 胡椒碱与棉酚联用对前列腺癌DU145细胞生长抑制的协同作用%Synergistic inhibition of growth of prostate cancer DU145 cells by combined piperine and gossypol treatment

    Institute of Scientific and Technical Information of China (English)

    曾龙辉; 徐丽慧; 何贤辉; 欧阳东云; 张延亭; 任帅

    2012-01-01

    目的 研究胡椒碱与棉酚联合用药对激素非依赖型前列腺癌DU145细胞生长的抑制作用及其机制.方法 MTS比色法检测细胞活性;流式细胞术分析细胞周期;免疫印迹法检测细胞周期相关蛋白以及CYP3A4的表达.结果 胡椒碱本身的细胞毒性较小,但与棉酚联用时,表现出协同作用.棉酚对细胞的半数抑制浓度IC50由单用时的21.00 μmol·L-1下降到联合用药时的8.07 μmol·L-1;两药相互作用指数CDI<1.胡椒碱与棉酚联合用药,可引起细胞G0/G1期阻滞和亚二倍体峰(凋亡峰)增加;同时上调细胞周期抑制蛋白p21Cip1的表达,并下调Cyclin D1、Cyclin A、Cyclin B1以及药物代谢酶CYP3A4的表达.结论 胡椒碱与棉酚联用,可能是通过诱导细胞周期阻滞,增强凋亡以及抑制CYP3A4药物代谢酶活性,发挥其协同抗前列腺癌作用.%Aim To investigate the effect of combined piperine and gossypol in inhibition of the growth of an-drogen-independent prostate cancer DU145 cells and e-lucidate the underlying mechanism. Methods MTS assay was adopted to measure the cell viability. Cell cycle distribution was analyzed by flow cytometry. Western blotting was used to determine the expression of cell cycle related proteins and CYP3A4. Results Piperine was relatively low toxic to DU145 cells, whereas when it was used in combination with gossypol, the IC50 of gossypol was decreased from 21. 00 μmol · L-1 to 8. 07 μmol · L-1 . The coefficient of drug interaction was less than 1, demonstrating that piperine plus gossypol had a synergistic effect on inhi- bition of the growth of DU145 cells. The combined drugs also significantly arrested cells at G0/G1 phase, and increased sub-G0/G1 peak (apoptotic peak). Moreover, the combined piperine and gossypol also results in an increased level of p21Cip1 expression and decreased expression levels of Cyclin D1, Cyclin A, Cyc-lin B1 and drug-metabolic enzyme CYP3A4. Conclusion The combination of

  7. Atlas du Liban

    Directory of Open Access Journals (Sweden)

    Ramez Philippe Maalouf

    2008-11-01

    Full Text Available Compte-rendu de l’ouvrage Atlas du Liban: territoires et société, sous la direction d’Éric Verdeil, Ghaleb Faour et Sébastien Velut, édition franco-libanaise de l’IFPO (Institut Français du Proche-Orient et du CNRS Liban (Conseil National de la Recherche Scientifique – Liban, Beyrouth 2007.Resenha do livro Atlas du Liban: territoires et société, sob a direção de Éric Verdeil, Ghaleb Faour e Sébastien Velut, editado por iniciativa franco-libanesa do IFPO (Institut Français du Proche-Orient e pelo CNRS Liban (Conseil National de la Recherche Scientifique – Liban, Beirute, 2007.Review of Atlas du Liban: territoires et société, edited by Éric Verdeil, Ghaleb Faour and Sébastien Velut, french-lebanese edition by IFPO (Institut Français du Proche-Orient and CNRS Liban (Conseil National de la Recherche Scientifique – Liban Beirut, 2007.

  8. Ecologie du phytoplancton du lac Kivu

    Directory of Open Access Journals (Sweden)

    Sarmento, H.

    2008-01-01

    Full Text Available Speciation within the African Coffee Pathogen. Cet article analyse s'il est avantageux d'utiliser le compost au lieu de l'engrais minéral pour produire la laitue dans la zone urbaine et péri-urbaine de Yaoundé. Les résultats de terrain montrent l'obtention de rendements et profits plus élevés lorsqu'on utilise le compost. Les résultats de la fonction de production Cobb-Douglas prouvent que l'utilisation du compost est statistiquement significative pour expliquer la variation de rendement de la laitue et que le compost est l'intrant le plus productif. D'autres résultats montrent que le compost fournit la matière organique utile au sol et que les besoins d'irrigation en eau de la culture sont réduits grâce à l'utilisation du compost. Par conséquent, malgré le fait que l'application du compost demande une main-d'oeuvre beaucoup plus élevée, son utilisation est généralement bénéfique pour les agriculteurs vivant aux alentours de Yaoundé. Les programmes de vulgarisation de cet intrant pour encourager son adoption devraient donc figurer parmi les points prioritaires dans la politique agricole du gouvernement camerounais.

  9. No association between polymorphisms and haplotypes of COL1A1 and COL1A2 genes and osteoporotic fracture in postmenopausal Chinese women

    Institute of Scientific and Technical Information of China (English)

    Wei-wei HU; Miao LI; Yu-juan LIU; Zhen-Iin ZHANG; Jin-wei HE; Hao ZHANG; Chun WANG; Jie-mei GU; Hua YUE; Yao-hua KE; Yun-qiu HU; Wen-zhen FU

    2011-01-01

    Aim: To study whether genetic polymorphisms of COL1A1 and COL1A2 genes affected the onset of fracture in postmenopausal Chinese women.Methods: SNPs in COL1A1 and COL1A2 genes were identified via direct sequencing in 32 unrelated postmenopausal Chinese women.Ten SNPs were genotyped in 1252 postmenopausal Chinese women. The associations were examined using both single-SNP and hapIotype tests using logistic regression.Results: Twenty four (4 novel) and 28 (7 novel) SNPs were identified in COL1A1 and COL1A2 gene, respectively. The distribution frequencies of 2 SNPs in COL1A1 (rs2075554 and rs2586494) and 3 SNPs in COL1A2 (rs42517, rs1801182, and rs42524) were significantly different from those documented for the European Caucasian population. No significant difference was observed between fracture and control groups with respect to allele frequency or genotype distribution in 9 selected SNPs and haplotype. No significant association was found between fragility fracture and each SNP or haplotype. The results remained the same after additional corrections for other risk factors such as weight, height, and bone mineral density.Conclusion: Our results show no association between common genetic variations of COL1A1 and COL1A2 genes and fracture, suggesting the complex genetic background of osteoporotic fractures.

  10. Types du Caucase

    OpenAIRE

    Makhacheva, Taus

    2015-01-01

    Tiré du site Internet de Onestar Press: "Types du Caucase - antique postcard collection/ 2013 to the present time. Coming from the personal archive of the artist, these postcards date back to the 19th century and can be considered representative for the "popularized ethnography" of the Russian Empire. Their primitive typology follows the classical rules of exotization : they depict "pittoresque" groups from various tribes, families or nationalities, or representatives of diverse professions. ...

  11. Les outils du CERN

    CERN Multimedia

    1999-01-01

    C'est le plus grand centre mondial de recherche en physique des particules. Les outils du Laboratoire, accélérateurs et détecteurs de particules, figurent parmi les instruments scientifiques les plus complexes au monde. Des prix Nobels ont d'ailleurs été attribués aux physiciens du CERN pour leurs développements.

  12. A comparative study on in vitro osteogenic priming potential of electron spun scaffold PLLA/HA/Col, PLLA/HA, and PLLA/Col for tissue engineering application.

    Science.gov (United States)

    Balaji Raghavendran, Hanumantha Rao; Puvaneswary, Subramaniam; Talebian, Sepehr; Murali, Malliga R; Raman Murali, Malliga; Naveen, Sangeetha V; Vasudevaraj Naveen, Sangeetha; Krishnamurithy, G; McKean, Robert; Kamarul, Tunku

    2014-01-01

    A comparative study on the in vitro osteogenic potential of electrospun poly-L-lactide/hydroxyapatite/collagen (PLLA/HA/Col, PLLA/HA, and PLLA/Col) scaffolds was conducted. The morphology, chemical composition, and surface roughness of the fibrous scaffolds were examined. Furthermore, cell attachment, distribution, morphology, mineralization, extracellular matrix protein localization, and gene expression of human mesenchymal stromal cells (hMSCs) differentiated on the fibrous scaffolds PLLA/Col/HA, PLLA/Col, and PLLA/HA were also analyzed. The electrospun scaffolds with a diameter of 200-950 nm demonstrated well-formed interconnected fibrous network structure, which supported the growth of hMSCs. When compared with PLLA/H%A and PLLA/Col scaffolds, PLLA/Col/HA scaffolds presented a higher density of viable cells and significant upregulation of genes associated with osteogenic lineage, which were achieved without the use of specific medium or growth factors. These results were supported by the elevated levels of calcium, osteocalcin, and mineralization (PCol/HA scaffolds exhibited superior osteoinductivity, when compared with PLLA/Col or PLLA/HA scaffolds. These findings indicated that the fibrous structure and synergistic action of Col and nano-HA with high-molecular-weight PLLA played a vital role in inducing osteogenic differentiation of hMSCs. The data obtained in this study demonstrated that the developed fibrous PLLA/Col/HA biocomposite scaffold may be supportive for stem cell based therapies for bone repair, when compared with the other two scaffolds.

  13. Tras la estela de Colón

    OpenAIRE

    Carmen Martínez Martin

    2011-01-01

    El más trascendental y conocido de los viajes de exploración llevado a cabo en la era de los grandes descubrimientos fue el protagonizado por Cristóbal Colón, quien, con el apoyo de la corona de Castilla, logró atravesar el océano Atlántico y descubrir el nuevo mundo.

  14. ({sup 18}F)-fluorodeoxyglucose PET/CT in cervix cancer: Lymph node assessment and prognostic/predictive value of primary tumour analysis; Tomographie par emission de positons au ({sup 18}F)-fluorodesoxyglucose dans les cancers du col uterin: evaluation ganglionnaire et valeur pronostique/predictive des donnees de la tumeur primitive

    Energy Technology Data Exchange (ETDEWEB)

    Leseur, J.; Williaume, D.; Le Prise, E.; De Crevoisier, R. [Departement des radiations, centre Eugene-Marquis, rue de la Bataille-Flandres-Dunkerque, CS 44229, 35042 Rennes cedex (France); Devillers, A.; Garin, E. [Service de medecine nucleaire, centre Eugene-Marquis, rue de la Bataille-Flandres-Dunkerque, CS 44229, 35042 Rennes cedex (France); Fougerou, C. [Service de pharmacologie, CHU de Rennes, 35033 Rennes cedex 09 (France); Inserm 0203, centre d' investigations cliniques, CHU de Rennes, 35033 Rennes cedex 09 (France); Universite de Rennes 1, CS 46510, 35065 Rennes cedex (France); Bouriel, C. [Service de radiologie, centre Eugene-Marquis, rue de la Bataille-Flandres-Dunkerque, CS 44229, 35042 Rennes cedex (France); Leveque, J. [Departement de gynecologie et obstetrique, CHU Anne-de-Bretagne, 16, boulevard de Bulgarie, 35203 Rennes cedex 2 (France); Monpetit, E. [Departement des radiations, clinique Oceane, 11, rue du Docteur-Joseph-Audic, Le Tenenio, BP 50020, 56001 Vannes cedex (France); Blanchot, J. [Departement de gynecologie et obstetrique, clinique mutualiste La Sagesse, 4, place Saint-Guenole, CS 44345, 35043 Rennes cedex (France)

    2011-12-15

    Purpose. - In cervix carcinoma: (a) to evaluate the ability of ({sup 18}F)-fluorodeoxyglucose (FDG) positron emission tomography (PET) in the lymph node detection; (b) to investigate the prognostic and predictive value of the primary cervical PET parameters. Patients and methods. - Ninety patients treated for cervix carcinoma and evaluated initially by MRI and FDG PET were included. The performances of FDG-PET for lymph node detection (relatively to the lymph node dissection) have been described (sensitivity, specificity, positive predictive value and negative predictive value). PET tumour parameters analyzed were: maximum standard uptake value (SUV{sub max}), the volume and the maximum diameter. The prognostic and predictive values of these parameters were investigated. The tumour response was evaluated on surgical specimens. Results. - PET detected the cervical tumour with a sensitivity of 97% (mean values: SUV{sub max} = 15.8, volume = 27 mm{sup 3}, maximum diameter = 47). For the detection of the lymph nodes, the values of sensibility, specificity, positive predictive value and negative predictive value were: 86, 56, 69 and 78% in the pelvic, and 90, 67, 50 and 95% for the para-aortic area, respectively. The SUV{sub max} was correlated with histologic response (P = 0.04). The frequency of partial histological response was significantly higher for tumour SUV{sub max}> 10.9 (P = 0.017). The maximum PET diameter and pathologic response had an impact on disease-free survival and overall survival in multivariate analysis (P < 0.05). Conclusion. - PET has high sensitivity in detecting pelvic and para-aortic lymph nodes. Some primary cervical tumour PET parameters are useful as prognostic and predictive factors. (authors)

  15. Dietary Lecithin Decreases Skeletal Muscle COL1A1 and COL3A1 Gene Expression in Finisher Gilts

    Directory of Open Access Journals (Sweden)

    Henny Akit

    2016-06-01

    Full Text Available The purpose of this study was to investigate the effect of dietary lecithin on skeletal muscle gene expression of collagen precursors and enzymes involved in collagen synthesis and degradation. Finisher gilts with an average start weight of 55.9 ± 2.22 kg were fed diets containing either 0, 4, 20 or 80 g/kg soybean lecithin prior to harvest for six weeks and the rectus abdominis muscle gene expression profile was analyzed by quantitative real-time PCR. Lecithin treatment down-regulated Type I (α1 procollagen (COL1A1 and Type III (α1 procollagen (COL3A1 mRNA expression ( p < 0.05, respectively, indicating a decrease in the precursors for collagen synthesis. The α-subunit of prolyl 4-hydroxylase (P4H mRNA expression also tended to be down-regulated ( p = 0.056, indicating a decrease in collagen synthesis. Decreased matrix metalloproteinase-1 (MMP-1 mRNA expression may reflect a positive regulatory response to the reduced collagen synthesis in muscle from the pigs fed lecithin ( p = 0.035. Lecithin had no effect on tissue inhibitor metalloproteinase-1 (TIMP-1, matrix metalloproteinase-13 (MMP-13 and lysyl oxidase mRNA expression. In conclusion, lecithin down-regulated COL1A1 and COL3A1 as well as tended to down-regulate α-subunit P4H expression. However, determination of muscle collagen content and solubility are required to support the gene functions.

  16. Mutation Analysis of COL1A1 and COL1A2 in Fetuses with Osteogenesis Imperfecta Type II/III.

    Science.gov (United States)

    Wang, Wenbo; Wu, Qichang; Cao, Lin; Sun, Li; Xu, Yasong; Guo, Qiwei

    2015-01-27

    Aim: To analyze COL1A1/2 mutations in prenatal-onset OI for determine the proportion of mutations in type I collagen genes among prenatal onset OI and to provide additional data for genotype-phenotype analyses. Material and Methods: Ten cases of severe fetal short-limb dwarfism detected by antenatal ultrasonography were referred to our center. Before the termination of pregnancy, cordocentesis was performed for fetal karyotype and COL1A1/2 gene sequencing analysis. Postmortem radiographic examination was performed at all instances for definitive diagnosis. Results: COL1A1 and COL1A2 SNP and mutations were identified in all the cases. Among these, one synonymous SNP and four synonymous SNPs were recognized in COL1A1/2, respectively, seven cases have distinct heterozygous mutations and six new COL1A1/2 gene mutations were identified. Conclusion: There has been substantial progress in the identification of the molecular defects responsible for skeletal dysplasias. With the constant increase in the number of identified mutations in COL1A1 and COL1A2, genotype-phenotype correlation is becoming increasingly pertinent. © 2015 S. Karger AG, Basel.

  17. La profanation du montage

    OpenAIRE

    Hildebrandt, Toni

    2017-01-01

    Cet article examine les liens entre, pour l’aspect technique, le plan séquence et le montage, et, pour l’aspect philosophico-historique, la vie quotidienne et la politique mondiale, du point de vue d’une profanation capable de désamorcer la puissance et avec une attention particulière portée à l’unique film expérimental de Pasolini : La sequenza del fiore di (1968). Ce court métrage n’a pas seulement un statut spécial dans la filmographie de Pasolini, il marque aussi un tournant du tragique v...

  18. Colágeno na cartilagem osteoartrótica

    Directory of Open Access Journals (Sweden)

    Ana Paula P. Velosa

    2003-06-01

    Full Text Available A cartilagem articular é um tecido altamente especializado, composto por células, os condrócitos, e um conjunto de macromoléculas, como o colágeno e os proteoglicanos. O colágeno é uma proteína fibrilar que garante resistência ao tecido, enquanto os proteoglicanos têm a função de mola biológica, sendo responsáveis pela compressibilidade da cartilagem. A complexa interação entre estas duas proteínas garante a elasticidade. Estas características específicas da cartilagem são essenciais para amortecer as grandes forças de impacto a que as articulações diartrodiais estão submetidas, sem muito gasto de energia, visto tratar-se de um tecido avascular. Em processos artrósicos ocorre um desequilíbrio entre a produção de componentes da matriz extracelular e destruição pelas metaloproteases, levando à degradação e perda do tecido cartilaginoso. A fase inicial da osteoartrose é marcada por perda de fragmentos de proteoglicanos para o líquido sinovial, aumento dos colágenos tipo II e tipo VI, aparecimento dos colágenos I e III, não típicos da cartilagem, e diminuição do colágeno tipo IX, que é importante para manter a integridade da matriz extracelular, além do entumescimento da cartilagem. Como conseqüência, a cartilagem perde suas características específicas, levando a alterações na função articular. A evolução da doença promove diminuição significativa das proteínas, até mesmo do colágeno tipo XI, que tem localização mais interna na estrutura da fibrila heterotípica, e, portanto levando até a exposição do osso. Até o momento, o tratamento da osteoartrose está baseado principalmente no controle da dor e/ou inflamação, não diminuindo ou impedindo a degradação da cartilagem articular. Neste aspecto a perspectiva de tratamento futuro da osteoartrose estaria na utilização de inibidores das metaloproteases associadas a condroprotetores interferindo no "turnover" da cartilagem e

  19. Origine du vieux slave

    Directory of Open Access Journals (Sweden)

    Witold Mańczak

    2005-12-01

    Full Text Available Comme, pour prouver l'identité du vieux slave et du vieux bulgare, on invoque uniquement des critères phonétiques, rappelons que l'orientaliste allemand du XVIIe siècle Ludolf affirmait déjà que "die Sprachverwandtschaft offenbart sich nicht im Wörterbuch, sondern in der Grammatik"3. Pendant les 300 dernières années, tellement d'autorites ant approuvé !l’opinion de Ludolf qu'elle est devenue un dogme de la linguistique. Pourtant il nous est venu à l'esprit de le confronter avec des faits et ainsi nous sommes arrivé à la conclusion qu'en réalité, c'est le vocabulaire (et non la phonétique et la flexion qui décide du degre de la parenté des langues4. Voici des arguments à l'appui de cette thèse.

  20. La foret du Banco

    NARCIS (Netherlands)

    Koning, de J.

    1983-01-01

    Deze publicatie over het Nationaal Park 'La Forêt du Banco' nabij Abidjan, Ivoorkust, verschijnt als resultaat van vier jaar veldwerk (1972-1976) in dat land.Het Centre Néerlandais, onderzoeks- en stageverblijf van de Landbouwhogeschool, was basis en de faciliteiten verleend door het Franse ORSTOM-i

  1. Historique du Web

    CERN Multimedia

    TV8 Mont-Blanc

    1995-01-01

    Documentaire court qui retrace l'implication de différentes personalités à la création du Web. Entrevues avec Robert Cailliau, Chris Llewellyn-Smith, David Williams, Tim Berners-Lee, Mike Sendall, Brian Carpenter.

  2. Evolution of thyroid cancer occurrence in metropolitan France. Assessment over 25 years; evolution de l'incidence du cancer de la thyroide en France metropolitaine. Bilan sur 25 ans

    Energy Technology Data Exchange (ETDEWEB)

    Rogel, Agnes; Caserio-Schonemann, Celine; Cherie-Challine, Laurence; Rudant, Jeremie; Bloch, Juliette; Thuret, Anne [Unite cancer, Departement des maladies chroniques et traumatismes - DMCT, Institut de veille sanitaire - InVS (France); Colonna, Marc [Registre des cancers de l' Isere, Reseau francais des registres de cancer (Francim) (France); Uhry, Zoe; Kudjawu, Yao; Danzon, Arlette [Unite cancer, DMCT, InVS (France); Lacour, Brigitte [Registre national des tumeurs solides de l' enfant, Francim (France); Schvartz, Claire [Registre des cancers de la thyroide Marne-Ardennes, Francim (France); Pascal, Laurence; Lasalle, Jean-Luc [Cellule interregionale d' epidemiologie - Cire Sud (France); Borson-Chazot, Francoise; Sassolas, Genevieve; Hafdi-Nejjari, Zakia [Registre des cancers thyroidiens de la region Rhone-Alpes (France); Boutron-Ruault, Marie-Christine; Guenel, Pascal; Vathaire, Florent de; Guillas, Gwenaelle; Mesrine, Sylvie; Clavel-Chapelon, Francoise; Clero, Enora; Adjadj, Elisabeth; Bedouche, Lallia [Institut national de la sante et de la recherche medicale (Inserm) U1018 (France); Belot, Aurelien [Unite Cancer, DMCT, InVS (France); Hospices civils de Lyon - HCL (France); Fieffe, Sandrine; Dalac, Audrey; Goncalves, Katia; Kaplan, Martine; Pochart, Jean-Marie [Registre des cancers de la thyroide Marne-Ardennes, Francim, Centre de luttre contre le cancer de Reims (France); Desenclos, Jean-Claude [Direction scientifique, InVS (France)

    2011-04-15

    After a presentation of the epidemiological context of thyroid cancer in France, this report, based on cancer record data, analyzes the occurrence of thyroid cancers between 1982 and 2006. It discusses the contribution and limits of medical-administrative data for the epidemiological monitoring of thyroid cancer occurrence between 1997 and 2009. It proposes a descriptive analysis of thyroid cancers in two districts (Marne and Ardennes) between 1975 and 2008, and a descriptive analysis of thyroid cancer for children under 14 between 2000 and 2008. It proposes an estimation of thyroid cancer occurrence in Corsica between 1998 and 2006. It reports and discusses a pilot study performed in two regions (Ile de France and Nord Pas-de-Calais), based on a multi-source system of cancer monitoring (SMSC), and comments studies on risk factors for differentiated thyroid cancers in France

  3. Solanine inhibits prostate cancer Du145 xenograft growth in nude mice by inducing cell cycle arrest in G1/S phase%龙葵素通过诱导细胞周期G1/S阻滞抑制裸鼠前列腺癌细胞Du145移植瘤生长

    Institute of Scientific and Technical Information of China (English)

    钟伟枫; 刘思平; 潘斌; 唐兆烽; 钟锦光; 周芳坚

    2016-01-01

    Objective To investigate the effect of solanine on the growth of human prostate cancer cell xenograft in nude mice. Methods Human prostate cancer Du145 cells were injected into the subcutaneous layers on the back of nude mice. After a week, the mice bearing subcutaneous tumor graft were randomly divided into solanine treatment group and saline control group for treatment for 3 weeks. The tumor grafts were then harvested to evaluate the inhibition rate. The mRNA and protein expressions of cell cycle-related genes in the tumors were detected by qRT-PCR and Western blotting, respectively, and tumor cell apoptosis was detected using TUNEL method. Results The tumor growth rate in solanine-treated group was significantly slower than that in the control group (P<0.01). The mRNA and protein expressions of C-myc, cyclin D1, cyclin E1, CDK2, CDK4 and CDK6 were significantly inhibited by solanine. Solanine significantly up-regulated p21 mRNA and protein expression in the tumors and induced a higher apoptosis rate of the tumor cells than saline (P<0.01). Conclusion The tumor-inhibition effect of solanine is probably mediated by regulating the expressions of genes related with G1/S cell cycle arrest and cell apoptosis.%目的:探讨龙葵素对前列腺癌细胞Du145裸鼠移植瘤生长的影响及分子机制。方法采用高度恶性的转移前列腺癌细胞株Du145作为动物体内实验的模型,裸鼠皮下接种Du145细胞建立裸鼠皮下瘤模型。1周后将接种的裸鼠随机分为2组:龙葵素实验组和生理盐水空白对照组,每3 d分别向实体瘤中间部位注射0.2 mL龙葵素(50μg/mL)和生理盐水,观察裸鼠体内肿瘤生长,3周后颈椎脱臼处死裸鼠,剥离肿瘤组织,测量肿瘤的重量并根据肿瘤重量计算抑瘤率。实时荧光定量PCR和Western blotting技术检测各组裸鼠瘤体细胞周期相关基因mRNA和蛋白表达。Tunel原位检测各组裸鼠瘤体组织凋亡情况。结果龙葵素

  4. Cisto gigante de colédoco Giant choledochal cyst

    Directory of Open Access Journals (Sweden)

    Olival Cirilo Lucena da Fonseca-Neto

    2007-12-01

    Full Text Available INTRODUÇÃO: A doença cística das vias biliares é anomalia congênita que pode acometer as vias biliares intra e/ou extra-hepáticas. A extra-hepática inclui os cistos de colédoco e a intra-hepática é conhecida por Doença de Caroli. Os cistos de colédoco de tamanho gigante são muito raros. OBJETIVO: Relatar o caso de um cisto de colédoco de tamanho gigante em uma paciente feminina. RELATO DE CASO: Mulher de 19 anos foi admitida com história de icterícia e acolia fecal há sete dias. Referia dor epigástrica associada com ingestão de dieta rica em gordura. Nos antecedentes pessoais relatou dois episódios de icterícia, aos 8 e 14 anos, que progrediram espontaneamente. No exame físico apresentava icterícia (+3/+4 e uma massa palpável indolor em mesogástrio foram os únicos achados. A ultrassonografia demonstrou grande formação cística de paredes finas adjacente ao hepatocolédoco, pâncreas e rim direito que media 18,5 x 10,2 cm. A colangioressonância confirmou o grande cisto de colédoco e hepatojejunoanastomose em "Y" de Roux após excisão do cisto e colecistectomia foi realizada. A formação cística media 20 x 15,5 x 12,5 cm e com um volume médio de 1000 mL. A paciente encontra-se em acompanhamento ambulatorial sem alterações hepatobiliares após o sétimo mês da operação. CONCLUSÃO: O cisto de colédoco deve fazer parte do diagnóstico diferencial em pacientes adultos jovens com icterícia e massa palpável; no entanto, a diferenciação entre ele e neoplasia maligna deve ser pesquisada.BACKGROUND: Choledochal cyst represents a rare congenital anomaly, eventually associated with intra and extrahepatic biliary tract disorders. Extrahepatic diseases include choledochal cysts and congenital dilation of the lower intrahepatic bile duct is known as Caroli's disease. Giant choledochal cyst constitutes a very rare abnormality. AIM:To report a giant choledochal cyst in a female patient. CASE REPORT: A 19-year

  5. New perspectives for radiosensitization in pancreatic carcinoma: A review of mechanisms involved in pancreatic tumorigenesis; Mecanismes de carcinogenese des cancers du pancreas: quelles pistes pour la radiosensibilisation?

    Energy Technology Data Exchange (ETDEWEB)

    Huguet, F. [Service d' oncologie-radiotherapie, hopital Tenon, Assistance publique-Hopitaux de Paris, 4, rue de la Chine, 75020 Paris (France); Universite Pierre-et-Marie-Curie Paris 6, 4, place Jussieu, 75005 Paris (France); Centre de recherche, institut Curie, campus universitaire, 91898 Orsay cedex (France); Inserm U612, campus universitaire, 91898 Orsay cedex (France); Fernet, M.; Favaudon, V. [Centre de recherche, institut Curie, campus universitaire, 91898 Orsay cedex (France); Inserm U612, campus universitaire, 91898 Orsay cedex (France); Monnier, L.; Touboul, E. [Service d' oncologie-radiotherapie, hopital Tenon, Assistance publique-Hopitaux de Paris, 4, rue de la Chine, 75020 Paris (France); Universite Pierre-et-Marie-Curie Paris 6, 4, place Jussieu, 75005 Paris (France)

    2011-08-15

    Pancreatic carcinoma is the fifth leading cause of cancer-related mortality. The 5-year overall survival is less than 5 %. This very poor prognosis can be explained both by late diagnosis and by treatment resistance, including resistance to radiation therapy. A better understanding of the pancreatic tumorigenesis and knowledge of the most frequent mutations in pancreatic adenocarcinoma (KRAS, p16, TP53, Smad4) open new perspectives for the development of more effective treatments. This review presents the major genetic and molecular alterations in pancreatic cancer that could be targeted to improve radiosensitization. (authors)

  6. A Study on the Dynamic Mechanism of the Formation of Mesoscale Vortex in Col Field

    Institute of Scientific and Technical Information of China (English)

    JIANG Yongqiang; WANG Yuan; HUANG Hong

    2012-01-01

    The mesoscale vortex associated with a mesoscale low-level jet (mLLJ) usually causes heavy rainfall in the col field.The col field is defined as a region between two highs and two lows,with the isobaric surface similar to a col.Using a two-dimensional shallow water model,the meso-β scale vortex couplets (MβVCs)induced by eight types of mesoscale wind perturbations in an ideal col field were numerically simulated.With the sizes of ~100 km,the MβVCs induced by northerly perturbation (NP) and southerly perturbation (SP)moved toward the col point.The sizes of MβVCs induced by southwesterly perturbation (SWP),southeasterly perturbation (SEP),northwesterly perturbation (NWP),and northeasterly perturbation (NEP) were relatively small for the perturbations moving toward dilatation axis.The MβVC induced by easterly perturbation (EP) and westerly perturbation (WP) could not develop because they quickly moved away from the col point,before the circulation could form.The size of the circulation was determined by the distance between the vortex and the col point.The closer to the col point the vortex was,the larger the size of vortex.The comparisons of maximum vorticity and vorticity root mean square error (RMSE) of the NP,the SWP,and the WP show that the maximum vorticity and the vorticity RMSE of the NP decreased slower than other perturbations.Therefore,the weak environment of the col field favors the maintenance of vorticity and the formation of vortex.When a mesoscalc vortex forms near the col point or moves toward the col point,it may maintain a quasi-stationary state in the stable col field.

  7. Effects of COL8A1 siRNA on the proliferation, invasion and drug sensitivity to D-limonence treatment in hepatocarcinoma cells%COL8A1siRNA对肝癌细胞体外增殖、侵袭能力及右旋柠烯药敏性的影响

    Institute of Scientific and Technical Information of China (English)

    赵永福; 贾莉; 马振海; 刘用楫

    2011-01-01

    目的 应用RNA干扰技术下调小鼠肝癌细胞株(Hca-F)COL8A1的表达,通过观察癌细胞的体外增殖、侵袭能力,研究调节COL8A1表达对肿瘤细胞药敏性的影响.方法 采用RTPCR、Western Blot分析干扰前后Hca-F细胞中COL8A1的表达情况.用体外侵袭实验检测干扰前后Hca-F细胞的侵袭能力.用MTT法分析干扰前后Hca-F细胞的增殖情况及其对右旋柠烯(D-limonene)的药敏性.结果 Hca-F/RNAi细胞中COL8A1的表达与未给予RNA干扰处理的对照组及RNA干扰对照组相比出现明显下调;该细胞的增殖及穿过基质胶的侵袭能力下降;同时上调该细胞对D-limonene的药敏性.结论 小鼠肝癌细胞中COL8A1的表达与肿瘤细胞的增殖、侵袭及对抗肿瘤药物的敏感性密切相关,可能为肿瘤的化学治疗提供新靶点.%Objective To investigate the possible effects of COL8A1 on the proliferation, invasion and drug sensitivity of murine hepatocarcinoma cell line Hca-F, we used an RNA interference (RNAi) approach to silence COL8A1 expression. Methods The expression levels of COL8A1 in HcaF/siRNA cells were assessed by RT-PCR and Western blot. The inhibitory effect of RNAi on Hca-F cell invasion in vitro was demonstrated by ECM invasion assay. The in vitro proliferative ability and drug sensitivity of COL8A1-deficient cells were determined by MTT assay. Results The expression of COL8A1 was significantly reduced in COL8A1/siRNA cells after 30h transfection, compared with both the RNAi control and the Hca-F cells. The reduced COL8A1 expression also attenuated the proliferative, invasive ability, as well as increased drug sensitivity of Hca-F/siRNA cells. Conclusion Our current results indicate that the expression of COL8A1 functionally mediates tumor cell proliferation, invasion, and drug sensitivity, and is a potential target for therapeutic anti-cancer drugs.

  8. 红花水煎液内服对硬皮小鼠皮肤COL-Ⅰ、COL-Ⅲ表达的影响

    Institute of Scientific and Technical Information of China (English)

    杨欢欢; 吕军影; 黄李平; 严煜林; 何雪萍

    2014-01-01

    目的 观察红花对硬皮小鼠皮肤COL-Ⅰ、COL-Ⅲ表达的影响,探讨红花抗皮肤纤维化的作用机制.方法 采用博来霉素诱导建立硬皮小鼠模型,分别采用生理盐水(模型组)、青霉胺、低中高不同剂量红花水煎液内服治疗硬皮小鼠,共28 d.对照组小鼠背部皮下注射磷酸盐缓冲液.HE染色观察皮肤病理改变并测量真皮厚度,免疫组化检测COL-Ⅰ、COL-Ⅲ的含量表达.结果 造模各组硬皮小鼠真皮厚度增厚,纤维增粗、膨大,数量增多,排列紊乱,COL-Ⅰ、COL-Ⅲ的含量表达降低,模型组尤为显著,与对照组比较差异均有统计学意义(P<0.05);与模型组比较,青霉胺和红花水煎液各剂量组药物干预后真皮厚度及COL-Ⅰ、COL-Ⅲ的表达均有不同程度降低,差异显著(P<0.05);与青霉胺组比较,红花水煎液中、高剂量组真皮厚度及COL-Ⅰ、COL-Ⅲ的表达明显降低,差异有统计学意义(P<0.05).结论 红花水煎液内服可降低硬皮小鼠皮肤COL-Ⅰ、COL-Ⅲ的表达,从而改善硬皮小鼠皮肤纤维化程度.

  9. Aux origines du monde

    CERN Multimedia

    2004-01-01

    "C'est l'histoire d'une aventure humaine, scientifique, international qui a vu le jour il y a cinquante ans, aux confins de la Suisse et du département de l'Ain. Le plus grand laboratoire de physique des particules du monde, le Cern, a été fondé en 1954. Les festivités organisées à l occasion de cet anniversaire connaîtront leur point d'orgue le 16 octobre prochain, avec portes-ouvertes, accueil de personallités et inauguration d'un monumnet spécifique, le Globe de l'innovation" (2 pages)

  10. Images du Valais

    Directory of Open Access Journals (Sweden)

    Micheline COSINSCHI

    1995-06-01

    Full Text Available Une étude des revenus fiscaux ventilés par branches économiques permet de clore un ouvrage portant sur ce canton alpin suisse par excellence qu’est le Valais. Pris comme traceurs de l’activité régionale, l’analyse de leurs configurations spatiales permet une évaluation pouvant être considérée comme une mesure de l’efficacité différentielle du système socio-spatial valaisan. L’article décrit certains processus qui ont permis la réalisation du dernier chapitre d’un atlas portant sur le Valais.

  11. CHOEUR DU CERN

    CERN Multimedia

    CHOEUR DU CERN

    2010-01-01

    Les répétitions du chœur du CERN reprendront le mercredi 15 septembre à 20.00 heures à l’amphithéâtre principal – bâtiment 500. Au programme la préparation de notre concert de Noël avec la Missa Brevis, KV115, de Léopold Mozart et de la musique de Noël d’Europe. Les personnes qui aiment chanter, notamment des sopranes et des ténors, sont les bienvenues. Pour tout contact s’adresser à : Baudouin Bleus - (tél.CERN 767 82 44) -(baudouin.bleus@cern.ch) ou Martin Gatehouse ( martin.gatehouse@wanadoo.fr) ou Jean-Paul Diss (jean-pauldiss@wanadoo.fr).  

  12. La mesure du danger

    CERN Document Server

    Manceron, Vanessa; Revet, Sandrine

    2014-01-01

    La mesure du danger permet d’explorer des dangers de nature aussi diverse que la délinquance, la pollution, l’écueil maritime, la maladie ou l’attaque sorcellaire, l’extinction d’espèces animales ou végétales, voire de la Planète tout entière. Au croisement de la sociologie, de l’anthropologie et de l’histoire, les différents articles analysent les pratiques concrètes de mesure pour tenter de comprendre ce qui se produit au cours de l’opération d’évaluation du danger sans préjuger de la nature de celui-ci. L’anthropologie a contribué à la réflexion sur l’infortune en s’intéressant aux temporalités de l’après : maladies, catastrophes, pandémies, etc. et en cherchant à rendre compte de l’expérience des victimes, de leur vie ordinaire bouleversée, de la recomposition du quotidien. Elle s’intéresse aussi aux autres types de mesures, les savoirs incorporés, qui reposent sur l’odorat, la vue ou le toucher et ceux qui ressortent d’une épistémologie « non ...

  13. Har du sikret din alderdom?

    DEFF Research Database (Denmark)

    Kiens, Bente; Aagaard, Peter Gjerndrup

    2006-01-01

    Daglig fysisk aktivitet kan betragtes som indbetaling op din personlige helbredspension. Jo mere du sætter ind på kontoen, og jo oftere du gør det, jo større er sandsynligheden for, at du lever længere og får et sundere helbred. Og det er aldrig for sent at starte med indbetalingerne....

  14. An Energy Efficient MAC Proto col for Linear WSNs

    Institute of Scientific and Technical Information of China (English)

    DAI Guoyong; MIAO Chunyu; YING Kezhen; WANG Kai; CHEN Qingzhang

    2015-01-01

    Wireless sensor networks (WSNs) have been employed as an ideal solution in many applications for data gathering in harsh environment. Energy consumption is a key issue in wireless sensor networks since nodes are often battery operated. Medium access control (MAC) pro-tocol plays an important role in energy efficiency in wire-less sensor networks because nodes’ access to the shared medium is coordinated by the MAC layer. An energy ef-ficient MAC protocol is designed for data gathering in linear wireless sensor networks. In order to enhance the performance, when a source node transmits data to the sink, proper relay nodes are selected for forwarding data according to the energy consumption factor and residual energy balance factor. Some simulation experiments are conducted and the results show that, the proposed proto-col provides better energy efficiency and long lifetime than the existing DMAC protocol.

  15. EuroCirCol kick-off event

    CERN Multimedia

    Hardre, Julie

    2015-01-01

    The EuroCirCol (http://cern.ch/eurocircol) kick-off event at CERN on June 2-4 brought together 62 participants to constitute governance bodies, commit to the project plan and align the organisation, structures and processes of 16 institutions from 10 countries. The goal of the project is to conceive a post-LHC research infrastructure around a 100 km circular energy-frontier hadron collider capable of reaching 100 TeV collisions. The project officially started on June 1 and will run for four years. The total estimated budget of 11.2 million Euros includes a 2.99 million Euro contribution from the Horizon 2020 programme on developing new world-class research infrastructures (http://cordis.europa.eu/project/rcn/194962_en.html).

  16. Cytotoxic effects of tetracycline analogues (doxycycline, minocycline and COL-3) in acute myeloid leukemia HL-60 cells.

    Science.gov (United States)

    Song, Hairong; Fares, Mona; Maguire, Kim R; Sidén, Ake; Potácová, Zuzana

    2014-01-01

    Tetracycline analogues (TCNAs) have been shown to inhibit matrix metalloproteinases and to induce apoptosis in several cancer cell types. In the present study, the cytotoxic effects of TCNAs doxycycline (DOXY), minocycline (MINO) and chemically modified tetracycline-3 (COL-3) were investigated in the human acute myeloid leukemia HL-60 cell line. Cells were incubated with TCNAs in final concentrations of 0.5-100 µg/ml for 24 h. Viability of the leukemic cells was inhibited in a concentration-dependent manner using resazurin assay. The estimated IC50s were 9.2 µg/ml for DOXY, 9.9 µg/ml for MINO and 1.3 µg/ml for COL-3. All three TCNAs induced potent cytotoxic effects and cell death. Apoptosis, which was assessed by morphological changes and annexin V positivity, was concentration- and time-dependent following incubation with any one of the drugs. TCNAs induced DNA double strand breaks soon after treatment commenced as detected by γH2AX and western blot. The loss of mitochondrial membrane potential (Δψm), caspase activation and cleavage of PARP and Bcl-2 were observed; however, the sequence of events differed among the drugs. Pancaspase inhibitor Z-VAD-FMK improved survival of TCNAs-treated cells and decreased TCNAs-induced apoptosis. In summary, we demonstrated that TCNAs had a cytotoxic effect on the HL-60 leukemic cell line. Apoptosis was induced via mitochondria-mediated and caspase-dependent pathways in HL-60 cells by all three TCNAs. COL-3 exerted the strongest anti-proliferative and pro-apoptotic effects in concentrations that have been achieved in human plasma in reported clinical trials. These results indicate that there is a therapeutic potential of TCNAs in leukemia.

  17. Les pirogues du Maroni

    Directory of Open Access Journals (Sweden)

    Sophie François

    2012-04-01

    Full Text Available Sur le Maroni, fleuve frontière entre la Guyane française et le Surinam, les pirogues amarrées le long des berges se comptent par centaines. Elles représentent le seul moyen de transport pour les milliers de personnes vivant sur les rives du Maroni. Les différentes formes de pirogues sont révélatrices de la diversité géographique du Maroni et de la diversité culturelle des populations. Les Amérindiens du littoral naviguent uniquement dans l’estuaire du fleuve. Ils construisent des pirogues larges, hautes et terminées à la poupe par une haute étrave qui fend les vagues. Ces pirogues sont construites à partir d’une coque monoxyle expansée et rehaussée d’un ou de deux bordages. En chauffant la grume évidée, le bois acquiert une certaine plasticité qui permet d’écarter les flancs et d’obtenir une coque très large à partir d’un arbre de faible diamètre. Les Bushinenge, populations d’origine africaine qui ont déserté les grandes plantations de la Guyane hollandaise dès le début du XVIIe siècle et se sont installées sur les deux rives du Maroni, ont développé des formes de pirogues adaptées au passage des sauts. Leurs pirogues, construites également en expansant la coque au feu, sont étroites et longues et possèdent des extrémités curvilignes qui dépassent largement au–dessus du bordage. Les longues pirogues à moteur comme les canots–pagaies sont ornés de motifs d’entrelacs appelés tembé ; et de décors d’inspiration contemporaine. L’inventaire des pirogues du Maroni a mis en évidence la richesse et la vitalité du patrimoine nautique de cette région au début du XXIe siècle, tant au niveau des formes des embarcations que des décors et des pratiques de navigation.On the Maroni river, natural border between French Guyana and the Surinam, canoes tied to the river banks can be counted by the hundreds. They are the only mean of transportation for thousands of people living along the

  18. Initial predictive value for PET with FDG on the response to first chemotherapy of locally evolved uterine cervix carcinomas; Valeur de prediction de la TEP au FDG initiale sur la reponse a la chimioradiotherapie premiere des cancers du col uterin localement evolues

    Energy Technology Data Exchange (ETDEWEB)

    Chamois, J.; Peignaux, K.; Ciappuccini, R.; Bonnetain, F.; Brunotte, F.; Berriolo-Riedinger, A.; Maingon, P. [Centre Georges-Francois-Leclerc, 21 -Dijon (France)

    2007-11-15

    The initial value of the standardized uptake value is not statistically associated to the tumor response after chemoradiotherapy in the uterine cervix carcinomas. It is observed a trend to a better tumor response in case of S.U.V. ( standardized uptake value) higher on the PET before therapy. A study bearing on a more important number of patients is necessary to measure its predictive value. (N.C.)

  19. Comparative study vagina mould/ fletcher applicator in MRI guided pulsed rate brachytherapy in patients reached by an uterine cervix carcinoma; Etude comparative moule vaginal/applicateur de fletcher en curietherapie de debit pulse guide par IRM chez les patientes atteintes d'un cancer du col uterin

    Energy Technology Data Exchange (ETDEWEB)

    Francois, G.; Dumas, I.; Mazeron, R.; Verezesan, O.; Monnier, L.; Vieillot, S.; Gensse, M.C.; Haie-Meder, C. [Institut Gustave-Roussy, 94 - Villejuif (France)

    2009-10-15

    This study began in february 2009 and included until today ten patients. twenty patients are planned. The analysis of preliminary results show a much higher tolerance for the vaginal cast device. The dosimetry data were the object of a preliminary study that seems demonstrate an equivalence of the both modalities, with differences concerning the doses to critical organs; The definitive result swill be presented with the data including the twenty planned patients. (N.C.)

  20. Economic assessment of pulsed dose-rate (P.D.R.) brachytherapy with optimized dose distribution for cervix carcinoma;Evaluation economique de la curietherapie de debit pulse gynecologique (PDR) avec optimisation de la dose pour les cancers du col uterin

    Energy Technology Data Exchange (ETDEWEB)

    Remonnay, R.; Morelle, M.; Pommier, P.; Carrere, M.O. [Lyon Univ., 69 (France); Remonnay, R.; Morelle, M.; Pommier, P. [Axe Economie de la Sante, GATE, CNRS-UMR 5824, Centre Leon-Berard, 69 - Lyon (France); Pommier, P. [Centre Leon-Berard, 69 - Lyon (France); Haie-Meder, C. [Institut Gustave-Roussy, 94 - Villejuif (France); Quetin, P. [Centre Paul-Strauss, 67 - Strasbourg (France); Kerr, C. [Centre Val-d' Aurelle, parc Euromedecine, 34 - Montpellier (France); Delannes, M. [Institut Claudius-Regaud, 31 - Toulouse (France); Castelain, B. [Centre Oscar-Lambret, 59 - Lille (France); Peignaux, K. [Centre Georges Francois Leclerc, 21 - Dijon (France); Kirova, Y. [Institut Curie, 75 - Paris (France); Romestaing, P. [Centre hospitalier Lyon Sud, 69 - Pierre-Benite (France); Williaume, D. [Centre Eugene-Marquis, 35 - Rennes (France); Krzisch, C. [Hopital Sud, 80 - Amiens (France); Thomas, L. [Institut Bergonie, 33 - Bordeaux (France); Lang, P. [Groupe hospitalier Pitie-Salpetriere, 75 - Paris (France); Baron, M.H. [Hopital Jean-Minjoz, 25 - Besancon (France); Cussac, A. [Centre Rene-Gauducheau, 44 - Nantes-Saint-Herblain (France); Lesaunier, F. [Centre Francois-Baclesse, 14 - Caen (France); Maillard, S. [Institut Jean-Godinot, 51 - Reims (France); Barillot, I. [Hopital Bretonneau, 37 - Tours (France); Charra-Brunaud, C.; Peiffert, D. [Centre Alexis-Vautrin, 54 - Vandoeuvre-les-Nancy (France)

    2010-06-15

    Purpose: Our study aims at evaluating the cost of pulsed dose-rate (P.D.R.) brachytherapy with optimized dose distribution versus traditional treatments (iridium wires, cesium, non-optimized P.D.R.). Issues surrounding reimbursement were also explored. Materials and methods: This prospective, multi-centre, non-randomized study conducted in the framework of a project entitled 'Support Program for Costly Diagnostic and Therapeutic Innovations' involved 21 hospitals. Patients with cervix carcinoma received either classical brachytherapy or the innovation. The direct medical costs of staff and equipment, as well as the costs of radioactive sources, consumables and building renovation were evaluated from a hospital point of view using a micro costing approach. Subsequent costs per brachytherapy were compared between the four strategies. Results: The economic study included 463 patients over two years. The main resources categories associated with P.D.R. brachytherapy (whether optimized or not) were radioactive sources (1053 Euros) and source projectors (735 Euros). Optimized P.D.R. induced higher cost of imagery and dosimetry (respectively 130 Euros and 367 Euros) than non-optimized P.D.R. (47 Euros and 75 Euros). Extra costs of innovation over the less costly strategy (iridium wires) reached more than 2100 Euros per treatment, but could be reduced by half in the hypothesis of 40 patients treated per year (instead of 24 in the study). Conclusion: Aside from staff, imaging and dosimetry, the current hospital reimbursements largely underestimated the cost of innovation related to equipment and sources. (authors)

  1. Chemically modified tetracycline (COL-3) improves survival if given 12 but not 24 hours after cecal ligation and puncture.

    Science.gov (United States)

    Halter, Jeffrey M; Pavone, Lucio A; Steinberg, Jay M; Gatto, Louis A; DiRocco, Joseph; Landas, Steve; Nieman, Gary F

    2006-12-01

    Sepsis can result in excessive and maladaptive inflammation that is responsible for more than 215,00 deaths per year in the United State alone. Current strategies for reducing the morbidity and mortality associated with sepsis rely on treatment of the syndrome rather than prophylaxis. We have been investigating a modified tetracycline, COL-3, which can be given prophylactically to patients at high risk for developing sepsis. Our group has shown that COL-3 is very effect at preventing the sequelae of sepsis if given before or immediately after injury in both rat and porcine sepsis models. In this study, we wanted to determine the "treatment window" for COL-3 after injury at which it remains protective. Sepsis was induced by cecal ligation and puncture (CLP). Rats were anesthetized and placed into five groups: CLP (n = 20) = CLP without COL-3, sham (n = 5) = surgery without CLP or COL-3, COL3@6h (n = 10) = COL-3 given by gavage 6 h after CLP, COL3@12h (n = 10) = COL-3 given by gavage 12 h after CLP, and COL3@24h (n = 20) = COL-3 given by gavage 24 h after CLP. COL-3 that was given at 6 and 12 h after CLP significantly improved survival as compared with the CLP and the CLP@24h groups. Improved survival was associated with a significant improvement in lung pathology assessed morphologically. These data suggest that COL-3 can be given up to 12 h after trauma and remain effective.

  2. Breast cancer risk and residence near industry or traffic in Nassau and Suffolk Counties, Long Island, New York; Risque de cancer du sein a proximite d'installations industrielles ou du trafic routier dans les comtes de Nassau et de Suffolk (Long Island, New York)

    Energy Technology Data Exchange (ETDEWEB)

    Lewis-Michl, E.L.; Melius, J.M.; Kallenbach, L.R.; Ju, C.L.; Talbot, T.O.; Orr, M.F.; Lauridsen, P.E.

    1999-06-01

    The objectives of the study is the influence of the vicinity of an industrial area or an important road traffic, on the risk increase of a breast cancer. The results show a relation between the breast cancer for women after the menopause and a life near chemical industrial area.

  3. CANCER BRONCHO-PULMONAIRE ET thérapeutique

    OpenAIRE

    Chater, Asma

    2014-01-01

    Le cancer du poumon est une maladie caractérisée par une croissance incontrôlée des cellules dans les tissus du poumon. Si elle n'est pas traitée, cette croissance peut se propager au-delà du poumon dans un processus appelé métastase dans les tissus voisins et, éventuellement, dans d'autres parties du corps. La plupart des cancers qui commencent dans les poumons, connue sous le nom cancers du poumon primaires, sont des carcinomes qui dérivent de cellules épithéliales. Les principaux types de ...

  4. L'Origine du Monde

    CERN Multimedia

    CNET & Ecole Polytechnique Paris; Vincent Ferreira

    1996-01-01

    Création, théologie, science, découverte, recherche, religion, condition humaine.Avec Maurice Jacob physicien, Hubert Curien Président du Conseil du CERN, Michael Doser physicien, Frère Emile Communauté de Taizé.

  5. What is the most cost-effective strategy to screen for second primary colorectal cancers in male cancer survivors in Korea?

    Institute of Scientific and Technical Information of China (English)

    Sang Min Park; Sun-Young Kim; Craig C Earle; Seung-Yong Jeong; Young Ho Yun

    2009-01-01

    AIM: To identify a cost-effective strategy of second primary colorectal cancer (CRC) screening for cancer survivors in Korea using a decision-analytic model.METHODS: A Markov model estimated the clinical and economic consequences of a simulated 50-year-old male cancer survivors' cohort, and we compared the results of eight screening strategies: no screening, fecal occult blood test (FOBT) annually, FOBT every2 years, sigmoidoscopy every 5 years, double contrast barium enema every 5 years, and colonoscopy every 10 years (COL10), every 5 years (COL5), and every3 years (COL3). We included only direct medical costs, and our main outcome measures were discountedlifetime costs, life expectancy, and incremental cost-effectiveness ratio (ICER).RESULTS: In the base-case analysis, the non-dominated strategies in cancer survivors were COL5, and COL3. The ICER for COL3 in cancer survivors was $5593/life-year saved (LYS), and did not exceed $10000/LYS in one-way sensitivity analyses. If the risk of CRC in cancer survivors is at least two times higher than that in the general population, COL5 had an ICER of less than $10500/LYS among both good and poor prognosis of index cancer. If the age of cancer survivors starting CRC screening was decreased to 40 years, the ICER of COL5 was less than $7400/LYS regardless of screening compliance.CONCLUSION: Our study suggests that more strict and frequent recommendations for colonoscopy such as COL5 and COL3 could be considered as economically reasonable second primary CRC screening strategies for Korean male cancer survivors.

  6. Coléoptères nouveaux ou peu connus du Musée de Leyde

    NARCIS (Netherlands)

    Fairmaire, L.

    1887-01-01

    Long. 13 millim. — Elongatus, modice convexus, rufus, modice nitidus, capite infuscato, fulvo-pubescens, subtus cum pedibus dilute rufescens, tarsis obscuris; capite summo fortiter punctato, antice transversim late impresso et subtilius sed densius punctato, labro rufescente, late sinuato; antennis

  7. Coléoptères nouveaux de l’Afrique du Musée de Leyde

    NARCIS (Netherlands)

    Fairmaire, L.

    1888-01-01

    Long. 22 à 25 mill. — Elongatus, convexus, fuscus aut brunneo-castaneus, modice nitidus, fulvo-pubescens; capite dense punctato, inter oculos sat late impresso, antice declivi, margine obtuse rotundato; antennis fuscis, medium corporis attingentibus, articulo 1° punctato, 2° minuto, 3° vix majore, a

  8. discours du Directeur General

    CERN Document Server

    CERN. Geneva

    2007-01-01

    L'année 2007 est une année très spéciale pour le CERN. Je vous propose de nous retrouver pour faire le point sur les activités en cours. Rendez-vous le mercredi 27 juin à 15h00 dans l'amphithéâtre principal. La transmission simultanée sera assurée dans les amphithéâtres IT, AT, AB Prevessin et dans la Salle du Conseil. Robert Aymar

  9. Soigner par la nature à la Réunion : l’usage des plantes médicinales comme recours thérapeutique dans la prise en charge du cancer Using Nature to Heal in the Reunion: Medicinal Plants as Therapeutic Recourse in Cancer Care Management

    Directory of Open Access Journals (Sweden)

    Caroline Desprès

    2011-06-01

    Full Text Available Cet article analyse les logiques profanes qui président à la consommation de plantes médicinales à l’île de la Réunion, dans le cadre de la prise en charge du cancer. Le contexte réunionnais est caractérisé par la pluralité des recours (médecine traditionnelle, biomédecine, médecines alternatives. Celle-ci est en particulier observée dans la prise en charge du cancer. Pour cette maladie dans l’immense majorité des cas, les recours se conjuguent à une prise en charge médicale. Les plantes médicinales occupent une place prépondérante au sein de la constellation de recours. Ces consommations de plantes habituellement sous forme de tisanes s’appuient sur des savoirs hérités et un habitus. Une fois le cancer diagnostiqué, certains malades vont abandonner l’usage de plantes dans la mesure où les médecins les contre-indiquent. D’autres au contraire vont consommer ces plantes afin d’accompagner la maladie ou aider à la guérison. Le rapport aux plantes apparait alors en lien avec la manière dont les malades s’inscrivent dans la prise en charge de leur maladie. Elle révèle une rupture de dépendance à l’égard de la médecine occidentale qui reste cependant partielle. L’usage des plantes peut prendre la signification d’une affirmation d’une identité culturelle dans un contexte de bouleversement de l’être provoqué par la maladie. Dans l’univers profane réunionnais, cet usage affranchi des prescriptions des tradipraticiens (tout en restant en continuité avec leurs savoirs renvoie à une démarche plus autonome dans la gestion individuelle des soins. Mais dans les deux cas, elle est un révélateur de stratégies de réappropriation de la maladie.This article analyses the profane logics underlying cancer patients’ medicinal plant consumption in the Reunion island (French department in the Indian Ocean. Care in the Réunionese context is characterized by the combined use of traditional

  10. Transcriptional regulation of acetylcholinesterase-associated collagen ColQ in fast- and slow-twitch muscle fibers.

    Science.gov (United States)

    Ting, Annie K L; Siow, Nina L; Kong, L W; Tsim, Karl W K

    2005-12-15

    The presence of a collagenous protein (ColQ) characterizes the collagen-tailed forms of acetylcholinesterase (AChE) and butyrylcholinesterase at vertebrate neuromuscular junctions, which is tethered in the synaptic basal lamina. ColQ subunits, differing mostly by their signal sequences, are encoded by transcripts ColQ-1 and ColQ-1a, which are differentially expressed in slow- and fast-twitch muscles in mammals, respectively. Both ColQ transcripts are derived from a single COLQ gene. Transcripts encoding ColQ increased during myogenic differentiation of C2C12 cells; the increase was in parallel with AChE catalytic subunit. Quantitative PCR analysis indicated that the increase during the myotube formation was due to the up regulation of ColQ-1 transcript instead of ColQ-1a. In order to reveal the regulatory mechanism of ColQ transcripts, two distinct promoters, pColQ-1 and pColQ-1a, were isolated from human COLQ gene. The ColQ promoters showed a muscle fiber type-specific expression pattern, and which was in line with the expression of endogenous transcript. After in vivo DNA transfection, pColQ-1 showed strong activity in slow-twitch muscle (e.g. soleus), while pColQ-1a was preferably expressed in fast-twitch muscle (e.g. tibialis). Mutation analysis of the ColQ promoters suggested that the muscle fiber type-specific expression pattern of ColQ transcripts was regulated by a slow upsteam regulatory element (SURE) and a fast intronic regulatory element (FIRE). These results explain the specific expression patterns of collagen-tailed AChE in slow and fast muscle fibers.

  11. Les risques du travail

    CERN Document Server

    Thébaud-Mony, Annie

    2015-01-01

    Depuis les années 1990, les conditions de travail se sont peu à peu imposées dans le débat social. Néanmoins, la situation reste critique. Les risques traditionnels n'ont pas disparu : les manutentions lourdes, l'exposition professionnelle aux cancérogènes, au bruit ou aux vibrations demeurent répandues... De plus, certaines " améliorations " n'ont fait que déplacer et dissimuler les problèmes, telle l'externalisation des risques grâce à la sous-traitance. Dans le même temps, les transformations du travail et des modalités de gestion de la main-d'œuvre ont fragilisé les collectifs et accru l'isolement des salariés, conduisant à une montée visible de la souffrance psychique. Face à ces évolutions, il est plus que jamais nécessaire que tous les acteurs concernés, en particulier les salariés eux-mêmes et leurs représentants, s'approprient les connaissances indispensables pour améliorer la protection de la santé sur les lieux du travail. Tel est le but de ce livre, qui renouvelle int�...

  12. Collimation and material science studies (ColMat) at GSI.

    CERN Document Server

    Stadlmann, J; Kollmus, H; Krause, M; Mustafin, E; Petzenhauser, I; Spiller, P; Strasik, I; Tahir, N; Tomut, M; Trautmann, C

    2010-01-01

    Within the frame of the EuCARD program, the GSI Helmholtzzentrum für Schwerionenforschung in Darmstadt is performing accelerator R&D in workpackage 8: ColMat. The coordinated effort is focussed on materials aspects important for building the FAIR accelerator facility at GSI and the LHC upgrade at CERN. Accelerator components and especially protection devices have to be operated in high dose environments. The radiation hazard occurs either by the primary proton and ion beams or the secondary radiation after initial beam loss. Detailed numerical simulations have been carried out to study the damage caused to solid targets by the full impact of the LHC beam as well as the SPS beam. Tungsten, copper and graphite as possible collimator materials have been studied. Experimental an theoretical studies on radiation damage on materials used for the LHC upgrade and the FAIR accelerators are performed at the present GSI experimental facilities. Technical decisions based on these results will have an impact on the F...

  13. Downregulation of Col1a1 induces differentiation in mouse spermatogonia

    Institute of Scientific and Technical Information of China (English)

    Sun-Hong Chen; Ding Li; Chen Xu

    2012-01-01

    Col1a1 (one of the subunit of collagen type Ⅰ) is a collagen,which belongs to a family of extracellular matrix (ECM) proteins that play an important role in cellular proliferation and differentiation.However,the role of Col1a1 in spermatogenesis,especially in the control of proliferation and differentiation of spermatogonial stem cells (SSCs),remains unknown.In this study,we explored effects of downregulation of Col1a1 on differentiation and proliferation of mouse spermatogonia.Loss-of-function study revealed that Oct4 and Plzf,markers of SSC self-renewal,were significantly decreased,whereas the expression of c-kit and haprin,hallmarks of SSC differentiation,was enhanced after Col1a1 knockdown.Cell cycle analyses indicated that two-thirds of spermatogonia were arrested in S phase after Col1a1 knockdown.In vivo experiments,DNA injection and electroporation of the testes showed that spermatogonia self-renewal ability was impaired remarkably with the loss-of-function of Col1a1.Our data suggest that silencing of Col1a1 can suppress spermatogonia self-renewal and promote spermatogonia differentiation.

  14. Postoperative radiotherapy after laser surgery with or without chemotherapy in head and neck evolved cancers; Radiotherapie postoperatoire apres chirurgie laser avec ou sans chimiotherapie dans les cancers evolues de la tete et du cou

    Energy Technology Data Exchange (ETDEWEB)

    Ryll, L.; Pradier, O. [Centre Hospitalier Universitaire Morvan, Dept. de Cancerologie, Brest (France); Nitsche, M.; Christiansen, H.; Hess, C. [Universitatsklinikum, Dept. de Cancerologie, Goettingen (Germany)

    2007-11-15

    We compared concurrent combination chemoradiotherapy and adjuvant radiotherapy after laser surgery in patients with stage 3/4 non metastatic squamous cell head and neck cancer. Combination chemotherapy and concurrent irradiation after laser surgery was not superior to surgery and postoperative radiotherapy for resectable advanced squamous cell head and neck cancer. However, the collective is small, and the follow-up to short to conclude. (authors)

  15. Jouer du piano

    Directory of Open Access Journals (Sweden)

    Fériel Kaddour

    2011-04-01

    Full Text Available La réflexion s’appuie dans un premier temps sur une opposition entre deux attitudes de pianistes  à l’égard du travail à l’instrument : Gould, qui revendique une séparation d’avec le clavier pour ne privilégier que la lecture; Arrau, dont la technique au contraire vise à « faire corps » avec son piano. L’étude de ces deux démarches d’interprètes conduit à une conclusion croisée : l’abstraction gouldienne n’est rien d’autre qu’un déplacement du jeu vers d’autres instruments (ceux qui servent à la prise de son et au montage de ses enregistrements ; le « faire-corps » hérité de la culture pianistique romantique est plus dialectique que fusionnel, et en cela implique une capacité de mise à distance. A partir de cette double conclusion, on tâche enfin de repenser la place du jeu à l’instrument dans la mise en œuvre d’une interprétation, en interrogeant le dialogue qui s’instaure entre la partition telle qu’elle s’écrit et le geste tel qu’il se joue.Our study leans on an opposition between two pianists' attitudes about their work with the instrument. Gould claims a necessary separation from the keyboard in order to prioritize reading. Arrau, on the contrary, relies on a technique which consists in “being one” with his piano. The analysis of these two interprets’ behaviours leads to a crossed conclusion: the gouldian abstraction is nothing else than a displacement of the playing towards another kind of instruments, the ones he uses in sound recording and cut up; Arrau’s “being one” is more dialectic than at first sight, and it therefore implies a real distancing from the piano. This constatation leads to rethink the place of the piano playing in the setting of an interpretation, and to highlight the real dialogue which develops itself between the score as it has been written and the gesture as it is played.

  16. Indoor radon exposure and lung cancer risk: a review of case-control studies; Exposition domestique au radon et risque de cancer du poumon: bilan des etudes cas-temoins

    Energy Technology Data Exchange (ETDEWEB)

    Baysson, H.; Tirmarche, M. [Institut de Radioprotection et de Surete Nucleaire (IRSN), Service de Radiobiologie et d' Epidemiologie, Dir. de la Radioprotection de l' Homme, 92 - Fontenay aux Roses (France)

    2004-04-01

    Background: Radon is a radioactive gas that tends to accumulate in indoor environment. A causal relationship between lung cancer and radon exposure has been demonstrated in epidemiologic studies of miners. The objective of this paper is to present the results of case-control studies of lung cancer risk associated with indoor radon exposure. Methods: Case-control studies published since 1990 are included in this review. This type of proto ol is particularly well suited for studying the relationship between indoor radon exposure and lung cancer risk, taking into account possible confounding factors such as tobacco smoking. The characteristics and results of these studies are summarized. The limitations associated with each of these studies are also discussed. Results: The results of available studies are relatively concordant and suggest a positive association between lung cancer risk and indoor radon exposure with an estimated excess relative risk of about 6 to 9% per 100 Bq/m{sup 3} increase in the observed time-weighted average radon concentration. The order of magnitude of this estimation agrees with extrapolations from miners but some studies may suffer from inadequate statistical power. Conclusion: At present, efforts are underway to pool together the data from the existing studies of indoor radon. This pooling analysis with thousands of cases and controls will provide a more precise estimate of the lung cancer risk from indoor radon exposure and explore the effect of modifying factors, such as smoking. (author)

  17. Lung cancer mortality among the miners in a rare-earth iron mine; La mortalite par cancer du poumon chez les mineurs d'une mine de terres-rares et de fer

    Energy Technology Data Exchange (ETDEWEB)

    Xing-An, C.; Yong-E, C. [National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing, P.R. (China)

    2008-07-15

    An epidemiology study on lung cancer mortality of the dust-exposed miners and dust-unexposed miners (controls) was performed in 2001. It was found that, during the period from 1977 to March 2001, 27 cases of lung cancers were observed in the dust-exposed miners, while 8 cases of lung cancers were found in controls. The S.M.R. of them were 6.13 and 1.90 respectively. The S.M.R. of the dust-exposed miners was much higher than that of the controls. The difference between the two S.M.R. is very significant ({chi}{sup 2} = 9.488, P < 0.005). The probability that 27 or more lung cancers would occur in the dust-exposed group, given the expected numbers and that a total of 35 occurred is 0.0015. The high S.M.R. for lung cancers among dust-exposed miners likely resulted from the inhaled thorium-containing dusts (carcinogens are ThO{sub 2} and SiO{sub 2}) and its short lived thoron progeny. This is the first evidence in humans of the carcinogenicity after long-term inhalation of thorium-containing dusts and thoron progeny. The total person-years of observation of the dust exposed miners and the unexposed miners were 62 712 and 34 672 respectively. (author)

  18. Collagen fibers mediate MRI-detected water diffusion and anisotropy in breast cancers.

    Science.gov (United States)

    Kakkad, Samata; Zhang, Jiangyang; Akhbardeh, Alireza; Jacob, Desmond; Krishnamachary, Balaji; Solaiyappan, Meiyappan; Jacobs, Michael A; Raman, Venu; Leibfritz, Dieter; Glunde, Kristine; Bhujwalla, Zaver M

    2016-10-01

    Collagen 1 (Col1) fibers play an important role in tumor interstitial macromolecular transport and cancer cell dissemination. Our goal was to understand the influence of Col1 fibers on water diffusion, and to examine the potential of using noninvasive diffusion tensor imaging (DTI) to indirectly detect Col1 fibers in breast lesions. We previously observed, in human MDA-MB-231 breast cancer xenografts engineered to fluoresce under hypoxia, relatively low amounts of Col1 fibers in fluorescent hypoxic regions. These xenograft tumors together with human breast cancer samples were used here to investigate the relationship between Col1 fibers, water diffusion and anisotropy, and hypoxia. Hypoxic low Col1 fiber containing regions showed decreased apparent diffusion coefficient (ADC) and fractional anisotropy (FA) compared to normoxic high Col1 fiber containing regions. Necrotic high Col1 fiber containing regions showed increased ADC with decreased FA values compared to normoxic viable high Col1 fiber regions that had increased ADC with increased FA values. A good agreement of ADC and FA patterns was observed between in vivo and ex vivo images. In human breast cancer specimens, ADC and FA decreased in low Col1 containing regions. Our data suggest that a decrease in ADC and FA values observed within a lesion could predict hypoxia, and a pattern of high ADC with low FA values could predict necrosis. Collectively the data identify the role of Col1 fibers in directed water movement and support expanding the evaluation of DTI parameters as surrogates for Col1 fiber patterns associated with specific tumor microenvironments as companion diagnostics and for staging.

  19. Potential risk and benefit of the combination of trastuzumab to chemotherapy and radiation therapy in non-metastatic breast cancer; Benefice et risques potentiels de l'association du trastuzumab a la chimiotherapie et a la radiotherapie dans le cancer du sein non metastatique

    Energy Technology Data Exchange (ETDEWEB)

    Belkacemi, Y. [CLCC Oscar-Lambret, Universite de Lille-2, Dept. d' Oncologie-Radiotherapie, 59 - Lille (France); Laharie-Mineur, H. [CLCC, institut Bergonie, 33 - Bordeaux (France); Gligorov, J. [APHP, Hopital Tenon, Cancer-Est, 75 - Paris (France); Azria, D. [CLCC Val d' Aurelle-Paul-Lamarque, Inserm, EMI 0227, 34 - Montpellier (France)

    2007-09-15

    Trastuzumab (Herceptin) is the first humanized monoclonal antibody targeting the HER2 antigen in breast cancer. HER2 receptor has been individualised 20 years ago. During the past 10 years, trastuzumab administration has radically modified the prognosis of the patients that are treated for HER2 positive breast cancer. Its efficacy has been demonstrated in the metastatic and adjuvant settings. While, trastuzumab based-regimens became the standard of care in the treatment of HER2/neu positive breast cancer, the optimal combination (concurrently or sequentially) to chemotherapy and radiation therapy is still unknown. Indeed, while the concurrent administration of trastuzumab and anthracyclines is not recommended because of a high risk of cardiac toxicity, there is no published data on the best sequence of trastuzumab and radiation therapy administration, particularly when internal mammary chain is involved. The benefit/risk ratio of the concurrent and sequential administration of trastuzumab with chemotherapy and radiation therapy will be discussed in this review. (authors)

  20. Le sacre du printemps

    Directory of Open Access Journals (Sweden)

    Denise Pumain

    2002-03-01

    Full Text Available Cybergeo aura six ans en avril : dans la réalité du virtuel, dans l'univers récent et fluctuant de la publication en ligne, cela fait de nous, tout à la fois, des pionniers et des vétérans. De façon plus surprenante, il se trouve que nous sommes aussi uniques : parmi toutes les revues électroniques de sciences sociales, aucune ne combine comme Cybergeo ancienneté, publication exclusivement électronique, liberté d'accès au texte intégral, édition et gestion par des chercheurs, et comité de lec...

  1. OPERATION DU FOISONNEMENT

    Directory of Open Access Journals (Sweden)

    Gholamreza Djelveh

    2010-04-01

    Full Text Available Mousses alimentaires sont un sous-ensemble des aliments connus sous le nom de produits fouettés ou des produits aérés. Ils sont des produits formulés avec des qualités telles que la légèreté et la souplesse et sont principalement consommés à l'apéritif ou au dessert. Les produits en mousse obtenue par dispersion d'un gaz dans une matrice alimentaire (la phase continue ont connu un développement croissant au cours des années 80 et 90. Le processus d'aération liés à leurs activités de production est appelée l'expansion ou à fouetter. Le document présente les principaux-paramètres du procédé du point permanent de la formulation, la mise en œuvre processus dans les installations pilotes et à l'échelle industrielle, la caractérisation des produits finis, la base énergétique de l'échelle de processus en place, et le lien entre la formulation, émulsion préparation de l'expansion. Cette vue d'ensemble de l'opération d'expansion continue, nous a permis de mettre en évidence le fait qu'il ya des opérations de l'unité encore mal décrite par le génie des procédés et pour lesquels les méthodes et outils pour l'extrapolation et la prédiction sont encore à leurs balbutiements.

  2. Male breast cancer: 22 case reports at the National Hospital of Niamey- Niger (West Africa Le cancer du sein chez l'homme: à propos de 22 cas a l'Hôpital National de Niamey- Niger (Afrique de l'Ouest

    Directory of Open Access Journals (Sweden)

    Nouhou Hassane

    2009-11-01

    Full Text Available BACKGROUND: Male breast cancer (MBC is rare. The objective of the study is to report clinicopathological characteristics, treatment patterns, and outcomes of MBC. METHODS: This study, which includes two parts (retrospective and prospective, focused on all hospitalized male patients with breast cancer during 17 years (1992–2008 with histological confirmation. RESULTS: The series included 22 patients. The mean age was 52.8 years (range: 28–80 years. MBC represented 5.7% of all breast cancers. Most patients had an advanced disease with skin ulceration and inflammation T3 (31.9% and T4 (59.1%. The majority of patients came from rural areas (63.6%. The duration of signs ranged from 1 to 7 years. Histology found infiltrating ductal carcinoma in 14 cases (63.6%, sarcoma in 3 cases (13.6%, papillary carcinoma in 2 cases (9%, and lobular carcinoma, medullar carcinoma, and mucinous carcinoma in 4.6% each of the others cases. The treatment had consisted of a radical mastectomy (Halsted or Patey in 19 cases (86.4% with axillary clearance and incomplete resection in 3 cases (13.6%. In the retrospective study follow-up of 14 patients, we lost sight of 13 patients 6 months after surgery. In the prospective study of 8 patients 10 to 36 months after mastectomy, 4 patients were deceased (50%, 4 were alive with 1 case having a local recurrence and pulmonary metastasis. CONCLUSION: The advanced clinical forms of MBC are most frequent with skin ulceration and nodal enlargement. The absence of radiotherapy and the low access of chemotherapy limited the treatment to radical mastectomy (Halsted in the majority of cases. OBJECTIF : Le cancer du sein chez l’homme (CSH est une affection rare .Le but de cette étude est de déterminer les caractéristiques clinicopathologiques, les résultats du traitement et l’évolution du CSH. Méthodologie : Il s’agit d’étude comportant 2 volets (rétrospectif et prospectif. Etaient inclus tous les patients de sexe

  3. Re-irradiation in stereotactic conditions of local relapses of non epidermoid head and neck cancers; Reirradiation en conditions stereotaxiques de recidives locales de cancers non-epidermoides de la tete et du cou

    Energy Technology Data Exchange (ETDEWEB)

    Mouttet, R.; Comet, B.; Faivre-Pierret, M.; Coche-Dequeant, B.; Degardin, M.; Lefebvre, J.L.; Lacornerie, T.; Lartigau, E. [Departement universitaire de radiotherapie, centre Oscar Lambret, 59 - Lille (France); Universite Lille-2, 59 (France)

    2010-10-15

    The authors report a study aimed at assessing the efficiency and toxicity of a re-irradiation performed in stereotactic conditions with CyberKnife of local relapses of non epidermoid cancers of head and neck. Twelve patients have been treated between July 2007 and July 2008 for different cancers. The obtained results and data show that this treatment presents acceptable early and late toxicities. Further studies are needed to assess the risk of longer term complications in comparison with other therapeutic techniques. Short communication

  4. “热敷药”对硬皮病小鼠模型COL-Ⅰ、COL-Ⅲ蛋白含量的影响%Effects of Refu Yao on Mice Models with Scleroderma of COL-Ⅰ and COL- Ⅲ

    Institute of Scientific and Technical Information of China (English)

    闫小宁; 韩世荣; 李文彬; 李美红; 刘燕婷

    2011-01-01

    Objective To study the effects of traditional Chinese herbs Refu Yao on mice models with scleroderma of COL-I and COL- M. Methods 60 BALB/C mice models were randomly divided into six groups with ten mice in each group. Mice models with scleroderma were established in mice by repeated local injections of bleomycin for five weeks. Then 10%, 20% and 40% Refu Yao were given to the mice models of high -concentration group, mid -concentration group and low concentration group for one month. Than the pathological change of skin tissue was observed, and the COL-I and COL- DI expression in lesions were detected by immunohistochemical detection. Results Compared with the model group, there was significant improvement in the skin tissue of high-concentration group and mid-concentration group. The content of COL-I and COL- ID was decreased remarkably by Refu Yao, and the difference was significance (PCOL-I and COL- DI, and it can also improve fibrosis in the skin.%目的 观察“热敷药”对硬皮病小鼠模型Ⅰ型胶原、Ⅲ型胶原(COL-Ⅰ、COL-Ⅲ)含量的影响.方法 60只BALB/c小鼠按照随机原则分为空白组、模型组、西药组、小浓度组、中浓度组、大浓度组各10只,采用博莱霉素皮下注射形成硬皮病模型,造模完成后,根据分组分别给予10%、20%、40%“热敷药”药糊及肝素钠软膏外用,1次/d,连续1个月,空白组及模型组不用药.完成后观察各组皮肤组织病理变化,免疫组化检测皮损COL-Ⅰ、COL-Ⅲ蛋白含量.结果 与模型组相比较,高、中浓度组硬皮痛小鼠模型COL-Ⅰ、COL-Ⅲ蛋白含量明显降低,差异较显著(P<0.05);结论 “热敷药”具有改善硬皮病小鼠模型皮肤硬化,降低COL-Ⅰ、COL-Ⅲ的作用.

  5. Changes of the MHC - Ⅱ and Col - Ⅰ , Col - Ⅲ in the Healing Process of Acute Skeletal Muscle Injury of Rats%大鼠骨骼肌急性拉伤修复过程中MHC-Ⅱ和COL -Ⅰ、COL -Ⅲ变化的研究

    Institute of Scientific and Technical Information of China (English)

    廖远朋

    2011-01-01

    目的:通过研究大鼠骨骼肌急性拉伤修复过程中MHC -Ⅱ和COL-Ⅰ、COL -Ⅲ的变化,了解拉伤愈合过程中肌纤维再生过程及纤维化过程.方法:将56只SD大鼠分为对照组、即刻组、第1、2、3、4、5周组,造成腓肠肌急性拉伤模型后对各组进行取材并检测MHC -Ⅱ和COL-Ⅰ、COL -Ⅲ.结果:MHC-Ⅱ水平第1周降到最低,第2周开始增加,第3周恢复正常;Col-Ⅰ水平第1周降到最低,随后逐渐增加,在第3周超过正常,持续到第5周;Col-Ⅲ从第1周开始上升,第3周达到峰值,随后逐渐下降.结论:骨骼肌急性拉伤后,骨骼肌的再生过程约在损伤l周后开始,伤后3周达到高峰,并持续5周以上.纤维化过程在损伤后1周以内开始,并持续到5周以后.其中Col-Ⅲ的水平恢复早;Col-Ⅰ的恢复较晚,但在高水平维持时间更长.

  6. Is the COL5A1 rs12722 gene polymorphism associated with running economy?

    Directory of Open Access Journals (Sweden)

    Rômulo Bertuzzi

    Full Text Available The COL5A1 rs12722 polymorphism is considered to be a novel genetic marker for endurance running performance. It has been postulated that COL5A1 rs12722 may influence the elasticity of tendons and the energetic cost of running. To date, there are no experimental data in the literature supporting the relationship between range of motion, running economy, and the COL5A1 rs12722 gene polymorphism. Therefore, the main purpose of the current study was to analyze the influence of the COL5A1rs12722 polymorphism on running economy and range of motion. One hundred and fifty (n = 150 physically active young men performed the following tests: a a maximal incremental treadmill test, b two constant-speed running tests (10 km · h(-1 and 12 km · h(-1 to determine the running economy, and c a sit-and-reach test to determine the range of motion. All of the subjects were genotyped for the COL5A1 rs12722 single-nucleotide polymorphism. The genotype frequencies were TT = 27.9%, CT = 55.8%, and CC = 16.3%. There were no significant differences between COL5A1 genotypes for running economy measured at 10 km · h(-1 (p = 0.232 and 12 km · h(-1 (p = 0.259. Similarly, there were no significant differences between COL5A1 genotypes for range of motion (p = 0.337. These findings suggest that the previous relationship reported between COL5A1 rs12722 genotypes and running endurance performance might not be mediated by the energetic cost of running.

  7. Place of imaging in the evaluation of treatment response in breast cancer;Place de l'imagerie dans l'evaluation de l'efficacite des traitements dans le cancer du sein

    Energy Technology Data Exchange (ETDEWEB)

    Brunotte, F.; Berriolo-Riedinger, A.; Cochet, A.; Toubeau, M.; Dygai-Cochet, I.; Riedinger, J.M. [Centre Georges-Francois-Leclerc, Service de medecine nucleaire, 21 - Dijon (France); Brunotte, F.; Cochet, A. [Universite de Bourgogne, UMR CNRS 5158, laboratoire electronique, informatique et image, 21 - Dijon (France)

    2010-01-15

    This paper describes, from the current literature, the role of various imaging methods to assess the response to therapy in breast cancer. Two different clinical situations are considered: neo-adjuvant chemotherapy of locally advanced breast cancer and the metastatic breast cancer. Significant clinical data are available for three criteria: the volume of the tumour, the uptake of fluorodeoxyglucose using PET and the perfusion of the tumor evaluated either by dynamic contrast-enhanced magnetic resonance imaging (D.C.E.-MRI) or by PET using {sup 15}O water. {sup 18}F F.D.G. PET allows prediction of the response after one or two cycles of neo-adjuvant chemotherapy. New approaches will offer opportunities to refine the role of imaging in monitoring the response to chemotherapy. PET using thymidine as bio marker is promising in assessing the tissular proliferation. Estrogen analogs could be used to predict hormonally responsive breast cancer. Many other approaches, although less developed, might offer new insights in the response to therapy of breast cancer like magnetic resonance spectroscopy or optical imaging of hemoglobin oxygenation. Imaging also offers potential of monitoring the down-regulation of specialized receptors of the cell membrane in response to treatment: the most studied receptor in preclinical model has been the human epidermal growth factor receptor type 2 (HER2). Integrin, a family of cell adhesion receptor, is also an important target for imaging. Apoptosis, multidrug resistance and hypoxia can also be studied using appropriate bio markers. To allow reliable multicenter trials of new drugs, these different imaging approaches still require an improved standardization of image acquisition and processing. (authors)

  8. Genetic polymorphisms in COL18A1 influence the development of osteosarcoma.

    Science.gov (United States)

    Guo, Zhihao; Zhang, Tianji; Wu, Juntao; Wang, Hongwei; Liu, Xiaotan; Tian, Linqiang

    2015-01-01

    We conducted a case-control study to investigate the association of COL18A1 D104N polymorphism in the development of osteosarcoma in a Chinese population. Between May 2012 and May 2014, 141 patients with pathologically proven osteosarcoma and 341 were selected into this study. Genotyping of COL18A1 D104N was analyzed using polymerase chain reaction (PCR) coupled with restriction fragment length polymorphism (RFLP). By logistic regression analysis, we found that individuals with the NN genotype of COL18A1 D104N were significantly associated with an increased risk of osteosarcoma when compared with the DD genotype (OR=20.97, 95% CI=2.74-933.42). In dominant model, the NN+DN genotype of COL18A1 D104N had a 1.99 fold risk of osteosarcoma when compared with the DD genotype. Moreover, the NN genotype was correlated with a 20.45 fold risk of osteosarcoma when compared with the DN+DD genotype in recessive model. However, we did not find significant interaction between COL18A1 D104N polymorphism and Enneking stage, histological subtype, tumor metastasis and tumor location of patients with osteosarcoma. In conclusion, our study suggests that the homozygous DN and NN genotypes of COL18A1 D104N were associated with the risk of osteosarcoma.

  9. A Novel COL4A5 Mutation Identified in a Chinese Han Family Using Exome Sequencing

    Directory of Open Access Journals (Sweden)

    Xiaofei Xiu

    2014-01-01

    Full Text Available Alport syndrome (AS is a monogenic disease of the basement membrane (BM, resulting in progressive renal failure due to glomerulonephropathy, variable sensorineural hearing loss, and ocular anomalies. It is caused by mutations in the collagen type IV alpha-3 gene (COL4A3, the collagen type IV alpha-4 gene (COL4A4, and the collagen type IV alpha-5 gene (COL4A5, which encodes type IV collagen α3, α4, and α5 chains, respectively. To explore the disease-related gene in a four-generation Chinese Han pedigree of AS, exome sequencing was conducted on the proband, and a novel deletion mutation c.499delC (p.Pro167Glnfs*36 in the COL4A5 gene was identified. This mutation, absent in 1,000 genomes project, HapMap, dbSNP132, YH1 databases, and 100 normal controls, cosegregated with patients in the family. Neither sensorineural hearing loss nor typical COL4A5-related ocular abnormalities (dot-and-fleck retinopathy, anterior lenticonus, and the rare posterior polymorphous corneal dystrophy were present in patients of this family. The phenotypes of patients in this AS family were characterized by early onset-age and rapidly developing into end-stage renal disease (ESRD. Our discovery broadens the mutation spectrum in the COL4A5 gene associated with AS, which may also shed new light on genetic counseling for AS.

  10. Experiencing Health Advocacy During Cervical Cancer Awareness Week: A National Initiative for Obstetrics and Gynaecology Residents.

    Science.gov (United States)

    Posner, Glenn; Finlayson, Sarah; Luna, Vilma; Miller, Dianne; Fung-Kee-Fung, Michael

    2015-07-01

    Objectif : Le Collège royal des médecins et chirurgiens du Canada exige que les résidents fassent preuve de compétence dans le rôle de promoteur de la santé (PS ou promotion de la santé). Nous avons cherché à élaborer et à mettre en œuvre, à l’intention des résidents en obstétrique-gynécologie, un module pédagogique national traitant de ce rôle de PS. Ce programme pilote était centré sur la prévention du cancer du col utérin, soit un sujet se prêtant bien à l’application des principes de la promotion de la santé. Méthodes : Un module pédagogique a été élaboré et transmis à tous les programmes de résidence en obstétrique-gynécologie au Canada. Ce module décrit les options de PS mettant en jeu le dépistage de la dysplasie cervicale (telles qu’une clinique visant l’élargissement de la population desservie ou un forum d’éducation visant le public / la population étudiante) qui devaient être mises en œuvre au cours de la Semaine de sensibilisation au cancer du col de l’utérus. La réussite a été mesurée en fonction du nombre de programmes mettant en œuvre le curriculum, du nombre de résidents y ayant participé, de la diversité des projets mis en œuvre, de la nature des personnes (patientes ou apprenants) atteintes par le programme et de l’expérience globale des stagiaires. Résultats : Trois programmes ont mis en œuvre le curriculum en 2011, un programme l’a fait en 2012 et sept l’ont fait en 2013. Après trois ans, le module s’est attiré la participation directe ou indirecte de sept des 16 facultés de médecine, de plus de 100 résidents et de milliers de femmes. De plus, les attributs de la PS vécus par les résidents ont été identifiés : travail d’équipe, leadership, connaissances accrues au sujet des systèmes, capital social accru au sein de la communauté, créativité, innovation et adaptabilité. Conclusion : Nous avons démontré qu’un module pédagogique, visant

  11. Exclusive radiotherapy and concurrent endocrine therapy for the management of elderly breast cancer patients: Case study and review of hypo-fractionated schemes; Hormonoradiotherapie exclusive dans la prise en charge du cancer du sein de la personne agee: cas clinique et revue de la litterature des schemas hypofractionnes

    Energy Technology Data Exchange (ETDEWEB)

    Auberdiac, P.; Cartier, L.; Malkoun, N.; Chauleur, C.; De Laroche, G.; Magne, N. [Departement de radiotherapie, institut de cancerologie de la Loire, 108 bis, avenue Albert-Raimond, BP 60008, 42271 Saint-Priest-en-Jarez cedex (France); Chargari, C. [Service d' oncologie radiotherapie, hopital d' instruction des armees du Val-de-Grace, 74, boulevard de Port-Royal, 75230 Paris cedex 5 (France); Melis, A.; Jacquin, J.P. [Departement d' oncologie medicale, institut de cancerologie de la Loire, 108 bis, avenue Albert-Raimond, BP 60008, 42271 Saint-Priest-en-Jarez cedex (France)

    2011-12-15

    Normo-fractionated radiotherapy is standard for adjuvant management of patients treated with breast conservative surgery for breast cancer. However, many elderly patients are not eligible to such strategy, either because of concurrent diseases, or because the tumor is inoperable. Several protocols of exclusive radiotherapy have been reported in the literature, frequently using hypo-fractionated radiotherapy and endocrine therapy. We report a case of a patient treated with exclusive endocrine and radiotherapy and address the state of the art on hypo-fractionated schemes for the management of elderly breast cancer patients. While hypo-fractionated radiotherapy does not compromise the oncologic or cosmetic outcome, there is no prospective data that assesses the place of radiotherapy for the exclusive treatment of elderly patients. This strategy should be further assessed in clinical randomized trial. (authors)

  12. Prediction of the response to the neo-adjuvant chemotherapy of breast cancer after two therapies of cyclophosphamide/epirubicin (E.C.) by PET-CT {sup 18}F-F.D.G; Prediction de la reponse a la chimiotherapie neoadjuvante du cancer du sein apres deux cures de cyclophosphamide/epirubicine (EC) par TEP-TDM {sup 18}F-FDG

    Energy Technology Data Exchange (ETDEWEB)

    Groheux, D.; Moretti, J.L.; Berenger, N.; Boin, C.; Hindie, E. [Hopital Saint-Louis, Service de medecine nucleaire, 75 - Paris (France); Espie, M.; Giacchetti, S.; Hamy, A.S.; Cuvier, C. [Hopital Saint-Louis, Service des maladies du sein, 75 - Paris (France); De Roquancourt, A. [Hopital Saint-Louis, Service d' anatomie pathologique, 75 - Paris (France)

    2010-07-01

    Purpose: in order to make easy the communication between clinician doctors and nuclear doctors, we created a standardized application of sentinel node scintigraphy requirement in case of breast cancer. Conclusions: experience shows that the transfer of information through the intermediary of the specific order only takes a minute. The specific order facilitates communication between medical doctors for the patients profit, with a budget of minimum time. (N.C.)

  13. Evaluation and management of second cancer risk in the modern radiotherapy elaboration of a ICRP/ICRU publication;Evaluation et gestion du risque de cancer secondaire dans la radiotherapie moderne elaboration d'une publication CIPR/ICRU

    Energy Technology Data Exchange (ETDEWEB)

    Cosset, J.M. [Institut Curie, Dept. de Radiotherapie, vice-president, comite 3 de l' ICRP, president du groupe de travail, 75 - Paris (France); Wambersie, A. [Membre de l' ICRU, co-president du groupe de travail, pour le groupe de travail ad hoc de la CIPR/ICRU, 75 - Paris (France)

    2009-12-15

    First the document analyses the available clinical data, by basing on the recent publication of the National Institute of Health (N.I.H.), 'New malignancies among cancer survivors'. Recent data on the second cancers appearing after a radiotherapy treatment seem confirm the clinical experience where it is said that the most of second cancers comes in the volume treated at high dose, or near this volume. The clinical data enhance the part of the age, children are more sensitive than adults. the second part of this document concerns the physical characteristics and the dose distributions appropriate to the different techniques. In order to compare the techniques the irradiated areas (out of the irradiated target volume itself) are divided in three groups: area receiving low doses <1 Gy, area receiving intermediate doses > 1 Gy and < 30 Gy, area receiving high doses >30 Gy (until the dose given to the target volume). The third part of the document treats the models of risk and the radiobiological aspects. The fourth part concerns the recommendations. It includes a chapter on the risk acceptability in the context of the treatment of a fatal disease. (N.C.)

  14. Prognostic value of initial F.D.G. PET in esophageal cancer; Valeur pronostique de la TEP au FDG dans le bilan initial du cancer de l'oesophage

    Energy Technology Data Exchange (ETDEWEB)

    Teyton, P.; Cheze-Le Rest, C. [CHU de Brest, Hopital Morvan, Service de Medecine Nucleaire, 29 - Brest (France); Metges, J.P.; Jestin-Le Tallec, V. [CHU de Brest, Hopital Morvan, Institut d' Oncologie, 29 - Brest (France); Lozac' h, P. [CHU de Brest, Hopital Cavale-Blanche, Service de Chirurgie, 29 - Brest (France); Volant, A. [CHU de Brest, Hopital Cavale-Blanche, Service d' Anatomopathologie, 29 - Brest (France); Visviki, D.; Cheze-Le Rest, C. [CHU de Brest, Inserm U650, 29 - Brest (France)

    2008-06-15

    Esophageal cancer outcome greatly depends on pathological stage. Our objectives were to assess prognosis based on the initial F.D.G. PET scan, focusing on correlation between overall survival and F.D.G. uptake in the primary, as well as the presence of F.D.G. positive lymph nodes or metastases. Fifty-two esophageal cancer patients undergoing F.D.G. PET as part of initial routine staging procedure before treatment were included. The maximum standardized uptake value (SUV{sub max}) was determined in each primary lesion and the number of abnormalities including primary, lymph nodes or distant metastases was recorded. Correlation with overall survival was performed using the Kaplan-Meier method and Cox regression analysis was used to assess the prognostic value of PET parameters. Half of the patients were planned for initial curative surgery (52%). Using univariate survival analysis, either surgery, SUV{sub max} superior than 9, two or more PET abnormalities, or the presence of F.D.G. positive nodes were significant overall survival prognostic predictors. After multivariate analysis, only SUV{sub max} superior than 9 and F.D.G. positive lymph nodes were found as independent predictors of poor outcome. In this prospective study F.D.G. PET was found to provide prognostic information supporting a new indication for initial F.D.G. PET examination in esophageal cancer. (authors)

  15. Proceedings from the DRG Seminar on Robotics in the Battlefield (31st) Held in Paris, France on 6-8 March 1991 (Acetes du 31ieme Seminaire sur la Robotique du Champ de Bataile)

    Science.gov (United States)

    1992-06-01

    volumes: - Volume A (U/U) contains: * The Executive Summary * The Opening Addresses by Dr. A. Grant, M. M. Bresson and Colonel Marescaux * The...A.J. Grant, Chairman of thc-Defence Research Group A2.1 -- A2.2 - M. M. Bresson , President d’lonneur du S~minaire A3.1 - A3.28 - Col. Marescaux...the SKIDS Project. IARP Ist Workshop on multisensor fusion and environment modeling, Toulouse France October 1989 And.d AYOUN, Cluiswphc BUR. Robert

  16. Scoliosis in osteogenesis imperfecta caused by COL1A1/COL1A2 mutations - genotype-phenotype correlations and effect of bisphosphonate treatment.

    Science.gov (United States)

    Sato, Atsuko; Ouellet, Jean; Muneta, Takeshi; Glorieux, Francis H; Rauch, Frank

    2016-05-01

    Bisphosphonates are widely used to treat children with osteogenesis imperfecta (OI), a bone fragility disorder that is most often caused by mutations in COL1A1 or COL1A2. However, it is unclear whether this treatment decreases the risk of developing scoliosis. We retrospectively evaluated spine radiographs and charts of 437 patients (227 female) with OI caused by mutations in COL1A1 or COL1A2 and compared the relationship between scoliosis, genotype and bisphosphonate treatment history. At the last follow-up (mean age 11.9 [SD: 5.9] years), 242 (55%) patients had scoliosis. The prevalence of scoliosis was highest in OI type III (89%), followed by OI type IV (61%) and OI type I (36%). Moderate to severe scoliosis (Cobb angle ≥25°) was rare in individuals with COL1A1 haploinsufficiency mutations but was present in about two fifth of patients with triple helical glycine substitutions or C-propeptide mutations. During the first 2 to 4years of bisphosphonate therapy, patients with OI type III had lower Cobb angle progression rates than before bisphosphonate treatment, whereas in OI types I and IV bisphosphonate treatment was not associated with a change in Cobb angle progression rates. At skeletal maturity, the prevalence of scoliosis (Cobb angle >10°) was similar in patients who had started bisphosphonate treatment early in life (before 5.0years of age) and in patients who had started therapy later (after the age of 10.0years) or had never received bisphosphonate therapy. Bisphosphonate treatment decreased progression rate of scoliosis in OI type III but there was no evidence of a positive effect on scoliosis in OI types I and IV. The prevalence of scoliosis at maturity was not influenced by the bisphosphonate treatment history in any OI type.

  17. Prevention of cancer and the dose-effect relationship: the carcinogenic effects of ionizing radiations; La prevention du cancer et la relation dose-effet: l'effet cancerogene des rayonnements ionisants

    Energy Technology Data Exchange (ETDEWEB)

    Tubiana, M. [Centre Antoine-Beclere, Faculte de Medecine, 75 - Paris (France)

    2009-07-15

    Cancer prevention has to be based on robust biological and epidemiological data, therefore its reappraisal becomes mandatory in view of recent progress in the understanding of carcinogenesis. The first phase of the carcinogenic process, that of initiation, is generally associated with mutation; however the role of extrinsic mutagens is less critical than was thought two decades ago. During intracellular oxygen metabolism, reactive oxygen species (R.O.S.) are made which are potent mutagens. Defense mechanisms against these intrinsic mutagens include scavenger and enzymatic systems which destroy them (catalase, superoxide dismutase). When the radiation dose is low, DNA repair is very effective as well as the elimination of cells with unrepaired or bad repaired DNA. Therefore a small increase in the number of R.O.S., such as that caused by a small dose of radiation has most probably no significant effect on the risk of DNA damage. These conclusions are consistent with the concept of a practical threshold. The second phase, that of promotion, appears to be the key one. During the promotion phase, initiated cells must acquire new properties (immortalization, release of angiogenic factors, resistance to hypoxia, etc.) in order to become pre-cancerous. This evolution is due to the accumulation in the genome of 6 to 10 new alteration defects. In the clone of initiated cells, the occurrence in one cell of a mutation or an epigenetic event gives birth to a sub clone. There is a Darwinian type competition between the sub clones and those with the more rapid growth because dominant (the acceleration of the growth rate can be due to shorter cell cycles or to an alleviation of cell proliferation exerted by the neighboring cells or the microenvironment). In the dominant sub clones new genomic events provoke the appearance of new sub clones growing more rapidly and having greater autonomy. The process is very slow because the specific genetic events that favour this evolution

  18. Le collisionneur du futur?

    CERN Multimedia

    CERN Audiovisual Service

    2009-01-01

    Pourquoi deux études parallèles pour un même collisionneur linéaire ? Loin d’être un double effort et un gaspillage de ressources, ces deux études s’inscrivent dans une stratégie de complémentarité afin de fournir la meilleure technologie requise par la physique du futur. Vendredi 12 juin a eu lieu au CERN la première réunion conjointe CLIC et ILC. Elle n’a pas été avare de bons résultats et d’importantes décisions. Le Collisionneur Linéaire International (ILC) et le Collisionneur Linéaire Compact (CLIC) sont deux études qui font, tous deux, appel à des technologies de pointe. A première vue en compétition, les deux études sont en réalité complémentaires et elles ont un objectif commun : proposer dans les plus brefs délais et au moindre coût, l‘accélérateur linéaire le mieux adapté pour prendre le relais de la physique des très hautes énergies après le LHC.

  19. Le bal du loup

    CERN Multimedia

    Happy Children's Home

    2013-01-01

    The Bord'eau amateur theatre group will graciously perform a play of their creation Le bal du loup Saturday 19 October 2013 at 20:00 Sunday 20 October at 17:00 in the Théâtre des Grottes Rue Louis Favre 43, 1201 Genève Children from age 12 upwards. Summary: The new-elected mayoress of a small village would like to clean up the town by prohibiting alcohol and getting rid of its prostitutes. Then along comes « Massimo Lupo » the pimp... The performances will be given to support the Happy Children's Home charity, which runs a foster-home in Pokhara for Nepali children:  http://www.happychildrenshome.org/ Admission : minimum charge of 10 CHF per person requested, to cover the cost of technical assistance and theatre rental. Any profit will be used solely for the foster-home. At the end of each performance members of the HCH charity will be happy to answer any questions you may have. The theatre has 86 seats, thank you for reserv...

  20. Development of a high resolution gamma imager for cancerology: from surgery treatment of cancer to the study on small animals; Developpement d'un imageur gamma haute resolution pour la cancerologie: du traitement chirurgical du cancer a l'etude sur petits animaux

    Energy Technology Data Exchange (ETDEWEB)

    Pitre, St

    2002-12-01

    In the context of the surgical treatment of cancer, counting probes of radioactivity have been introduced in a theater bloc to assist the surgeon in real time for the excision of the radio-labeled tumors. This technique of radio-guided surgery allows to reach the precise localization and the complete excision of pathological tissues. To reinforce this surgical practice we developed a mini gamma-camera called POCI (Per-Operative Compact Imager). The objective of this work was to determine the role of this new generation of detectors to assist the surgeon in the excision of tumors and to also approach cancer research involving studies on small animals. From the instrumental point of view, the principle of detection based on the photodiode with intensified localization has been validated in a first prototype which was extended to a large field of analysis imagery without degrading the spatial performances and with miniaturizing the dimensions of the camera. The prototype of the realized camera has a 40 mm diameter field of view and a total weight of 1.2 kg. At 140 keV, the spatial resolution is 2.1 mm for an efficiency of 2.8 10{sup -4}%. POCI was estimated through the sentinel node protocol in breast cancer staging according to two approaches: one based on a comparative study of the performances of detection of a probe and POCI and an other one based on a clinical evaluation in collaboration with Institute Gustave Roussy. This study has permit to establish the complementarity between the imager and the probe considering various clinical configurations. The detection performances of POCI were also estimated in mice to study the biodistribution of iodine in the thyroid and the mammary glands. All these encouraging results allows to consider the use of the detector in a wider frame of investigations clinical as well as biological. (author)

  1. Two different forms of lethal chondrodysplasias caused by COL2A1 gene mutations

    Energy Technology Data Exchange (ETDEWEB)

    Winterpacht, A.; Hilbert, K.; Schwarze, U. [Univ. of Mainz (Germany)] [and others

    1994-09-01

    Two bone dysplasia families seem to be due to mutations in the type II procollagen gene (COL2A1): the so-called spondyloepiphyseal dysplasia congenita (SEDC) group with achondrogenesis II, hypochondrogenesis, SEDC, osteoarthrosis and the Stickler-Kniest pattern that include different forms of Kniest and Stickler dysplasia. Both groups comprise a clinical spectrum ranging from lethal to mild. COL2A1-mutations have been identified in lethal forms of the SEDC family but not in lethal forms of the Stickler/Kniest group. We now report a COL2A-1 mutation in an additional case of hypochondrogenesis (patient S) and in a lethal case of Kniest dysplasia (patient B). We amplified all 54 exons of the COL2A1 gene in both patients and screened the PCR products for mutations by SSCP analysis and sequencing. In patient B, we identified an 18 bp deletion in exon 34 which removes 6 amino acids from the mature protein. In patient S, we were able to identify a two base pair exchange (GG to AT) in exon 31, which leads to the very unusual conversion of Gly to Ile. To our knowledge, this is the first report of a Gly to Ile conversion in the COL2A1 gene, and the first report of a COL2A1 gene mutation in a lethal form of Kniest dysplasia. On the basis of the known COL2A1 gene mutations and the genotype-phenotype correlations established so far, we provide molecular data (an in frame deletion in patient B and a Gly conversion in patient S) that support their clinical classification as Kniest dysplasia and hypochondrogenesis, respectively.

  2. Late xerostomia after intensity-modulated conformational radiotherapy of upper aero-digestive tract cancers: study 2004-03 by the head and neck oncology and radiotherapy Group (Gortec); Xerostomie tardive apres radiotherapie conformationnelle avec modulation d'intensite des cancers des voies aero-digestives superieures: etude 2004-03 du Groupe oncologie et radiotherapie de la tete et du cou (Gortec)

    Energy Technology Data Exchange (ETDEWEB)

    Toledano, I.; Lapeyre, M. [Centre Jean-Perrin, 63 - Clermont-Ferrand (France); Graff, P. [Centre Alexis-Vautrin, 54 - Vandoeuvre-les-Nancy (France); Serre, C. [Centre Val d' Aurelle, 34 - Montpellier (France); Bensadoun, R.J. [CHU La Miletrie, 86 - Poitiers (France); Bensadoun, R.J.; Ortholan, C. [Centre Antoine-Lacassagne, 06 - Nice (France); Calais, G. [CHU Bretonneau, 37 - Tours (France); Alfonsi, M. [Institut Sainte-Catherine, 84 - Avignon (France); Giraud, P. [Institut Curie, 75 - Paris (France); Hopital europeen Georges-Pompidou, 75 - Paris (France); Racadot, S. [Centre Leon-Berrard, 69 - Lyon (France)

    2010-10-15

    The authors report a retrospective assessment of late xerostomia according to the RTOG (Radiation Therapy Oncology Group) classification of the European Organization for Research and Treatment of Cancer (EORTC) among patients treated by intensity-modulated conformational radiotherapy (IMRT) and suffering from upper aero-digestive tract carcinomas of different stages. Some of these patients have bee operated, and some have been treated by chemotherapy. It appears that the IMRT results in a reduction of late xerostomia, and even in an absence of salivary toxicity. Short communication

  3. Effets du fluor et du phosphogypse chez les organismes marins

    OpenAIRE

    Martin, J.L.; Le Guellec, Anne-marie; Cosson, R.

    1982-01-01

    Dans le cadre général des recherches visant à déterminer l'action des effluents industriels et plus précisément du fluor dans le cas de cette étude, le programme dont les résultats sont présentés avait pour but de déterminer les effets de cet élément sur les organismes marins. Trois aspects essentiels ont été envisagés : - Effets létaux du fluor ; - effets du fluor sur certaines fonctions éthologiques ; - bio-accumulation de l'élément par les organismes marins.

  4. Main: 1DU5 [RPSD[Archive

    Lifescience Database Archive (English)

    Full Text Available 1DU5 トウモロコシ Corn Zea mays L. Zeamatin Precursor. Name=Zlp; Zea Mays Molecule: Zeama...NNACPVFKKDEYCCVGSAANDCHPTNYSRYFKGQCPDAYSYPKDDATSTFTCPAGTNYKVVFCP corn_1DU5.jpg ...

  5. Position of glycine substitutions in the triple helix of COL6A1, COL6A2, and COL6A3 is correlated with severity and mode of inheritance in collagen VI myopathies.

    Science.gov (United States)

    Butterfield, Russell J; Foley, A Reghan; Dastgir, Jahannaz; Asman, Stephanie; Dunn, Diane M; Zou, Yaqun; Hu, Ying; Donkervoort, Sandra; Flanigan, Kevin M; Swoboda, Kathryn J; Winder, Thomas L; Weiss, Robert B; Bönnemann, Carsten G

    2013-11-01

    Glycine substitutions in the conserved Gly-X-Y motif in the triple helical (TH) domain of collagen VI are the most commonly identified mutations in the collagen VI myopathies including Ullrich congenital muscular dystrophy, Bethlem myopathy, and intermediate (INT) phenotypes. We describe clinical and genetic characteristics of 97 individuals with glycine substitutions in the TH domain of COL6A1, COL6A2, or COL6A3 and add a review of 97 published cases, for a total of 194 cases. Clinical findings include severe, INT, and mild phenotypes even from patients with identical mutations. INT phenotypes were most common, accounting for almost half of patients, emphasizing the importance of INT phenotypes to the overall phenotypic spectrum. Glycine substitutions in the TH domain are heavily clustered in a short segment N-terminal to the 17th Gly-X-Y triplet, where they are acting as dominants. The most severe cases are clustered in an even smaller region including Gly-X-Y triplets 10-15, accounting for only 5% of the TH domain. Our findings suggest that clustering of glycine substitutions in the N-terminal region of collagen VI is not based on features of the primary sequence. We hypothesize that this region may represent a functional domain within the triple helix.

  6. Cri du Chat syndrome

    Directory of Open Access Journals (Sweden)

    Cerruti Mainardi Paola

    2006-09-01

    Full Text Available Abstract The Cri du Chat syndrome (CdCS is a genetic disease resulting from a deletion of variable size occurring on the short arm of chromosome 5 (5p-. The incidence ranges from 1:15,000 to 1:50,000 live-born infants. The main clinical features are a high-pitched monochromatic cry, microcephaly, broad nasal bridge, epicanthal folds, micrognathia, abnormal dermatoglyphics, and severe psychomotor and mental retardation. Malformations, although not very frequent, may be present: cardiac, neurological and renal abnormalities, preauricular tags, syndactyly, hypospadias, and cryptorchidism. Molecular cytogenetic analysis has allowed a cytogenetic and phenotypic map of 5p to be defined, even if results from the studies reported up to now are not completely in agreement. Genotype-phenotype correlation studies showed a clinical and cytogenetic variability. The identification of phenotypic subsets associated with a specific size and type of deletion is of diagnostic and prognostic relevance. Specific growth and psychomotor development charts have been established. Two genes, Semaphorin F (SEMAF and δ-catenin (CTNND2, which have been mapped to the "critical regions", are potentially involved in cerebral development and their deletion may be associated with mental retardation in CdCS patients. Deletion of the telomerase reverse transcriptase (hTERT gene, localised to 5p15.33, could contribute to the phenotypic changes in CdCS. The critical regions were recently refined by using array comparative genomic hybridisation. The cat-like cry critical region was further narrowed using quantitative polymerase chain reaction (PCR and three candidate genes were characterised in this region. The diagnosis is based on typical clinical manifestations. Karyotype analysis and, in doubtful cases, FISH analysis will confirm the diagnosis. There is no specific therapy for CdCS but early rehabilitative and educational interventions improve the prognosis and considerable

  7. Gestion durable du paysage

    Directory of Open Access Journals (Sweden)

    Jean-David Gerber

    2009-03-01

    Full Text Available Le paysage est de plus en plus perçu comme une ressource. À ce titre, il est nécessaire de trouver des instruments juridiques, politiques ou économiques susceptibles de gérer cette « ressource-paysage » sur le long terme. Le gouvernement suisse a introduit récemment l’instrument des parcs naturels régionaux, organisés selon le modèle français, dans sa législation de protection de la nature et du paysage. Une mise en regard des nouveaux parcs avec des structures de gestion beaucoup plus anciennes, les bourgeoisies et les corporations, permet de mettre en évidence les forces et les faiblesses de chacun de ces instruments dans leur contribution à résoudre les rivalités d’usage entre acteurs utilisant ou influençant la ressource paysage. Cette comparaison permet de formuler des recommandations pratiques concernant la gestion de cette ressource.The landscape is increasingly perceived as a resource. For this reason, it is necessary to find legal, political and economic instruments that will succeed in managing this “resource landscape” in the long term. The Swiss government recently introduced the instrument of regional nature parks into the legislation governing nature and landscape preservation; the proposed parks are organized on the basis of the French model. The examination of the new parks from the perspective of much older management structures, i.e. the civic municipalities (bourgeoisies and corporations, makes it possible to demonstrate the strengths and weaknesses of each of these instruments in their contribution to the resolution of use rivalries between actors who use or influence the resource landscape. This comparison also enables the formulation of practical recommendations regarding the management of this resource.

  8. A COL11A2 mutation in Labrador retrievers with mild disproportionate dwarfism.

    Directory of Open Access Journals (Sweden)

    Mirjam Frischknecht

    Full Text Available We describe a mild form of disproportionate dwarfism in Labrador Retrievers, which is not associated with any obvious health problems such as secondary arthrosis. We designate this phenotype as skeletal dysplasia 2 (SD2. It is inherited as a monogenic autosomal recessive trait with incomplete penetrance primarily in working lines of the Labrador Retriever breed. Using 23 cases and 37 controls we mapped the causative mutation by genome-wide association and homozygosity mapping to a 4.44 Mb interval on chromosome 12. We re-sequenced the genome of one affected dog at 30x coverage and detected 92 non-synonymous variants in the critical interval. Only two of these variants, located in the lymphotoxin A (LTA and collagen alpha-2(XI chain gene (COL11A2, respectively, were perfectly associated with the trait. Previously described COL11A2 variants in humans or mice lead to skeletal dysplasias and/or deafness. The dog variant associated with disproportionate dwarfism, COL11A2:c.143G>C or p.R48P, probably has only a minor effect on collagen XI function, which might explain the comparatively mild phenotype seen in our study. The identification of this candidate causative mutation thus widens the known phenotypic spectrum of COL11A2 mutations. We speculate that non-pathogenic COL11A2 variants might even contribute to the heritable variation in height.

  9. COL Application Content Guide for HTGRs: Revision to RG 1.206, Part 1 - Status Report

    Energy Technology Data Exchange (ETDEWEB)

    Wayne Moe

    2012-08-01

    A combined license (COL) application is required by the Nuclear Regulatory Commission (NRC) for all proposed nuclear plants. The information requirements for a COL application are set forth in 10 CFR 52.79, “Contents of Applications; Technical Information in Final Safety Analysis Report.” An applicant for a modular high temperature gas-cooled reactor (HTGR) must develop and submit for NRC review and approval a COL application which conforms to these requirements. The technical information necessary to allow NRC staff to evaluate a COL application and resolve all safety issues related to a proposed nuclear plant is detailed and comprehensive. To this, Regulatory Guide (RG) 1.206, “Combined License Applications for Nuclear Power Plants” (LWR Edition), was developed to assist light water reactor (LWR) applicants in incorporating and effectively formatting required information for COL application review (Ref. 1). However, the guidance prescribed in RG 1.206 presumes a LWR design proposal consistent with the systems and functions associated with large LWR power plants currently operating under NRC license.

  10. Case of Small Vessel Disease Associated with COL4A1 Mutations following Trauma

    Directory of Open Access Journals (Sweden)

    Joao McONeil Plancher

    2015-06-01

    Full Text Available With this case report, we would like to heighten the awareness of clinicians about COL4A1 as a single-gene disorder causing cerebral small vessel disease and describe a previously unreported pathogenic missense substitution in COL4A1 (p.Gly990Val and a new clinical presentation. We identified a heterozygous putatively pathogenic mutation of COL4A1 in a 50-year-old female with a history of congenital cataracts and glaucoma who presented with multiple diffusion-positive infarcts and areas of contrast enhancement following mild head trauma. We believe that this presentation of multiple areas of acute brain and vascular injury in the setting of mild head trauma is a new manifestation of this genetic disorder. Imaging findings of multiple acute infarcts and regions of contrast enhancement with associated asymptomatic old deep microhemorrhages and leukomalacia in adults after head trauma should raise a high suspicion for a COL4A1 genetic disorder. Radiographic patterns of significant leukoaraiosis and deep microhemorrhages can also be seen in patients with long-standing vasculopathy associated with hypertension, which our patient lacked. Our findings demonstrate the utility of genetic screening for COL4A1 mutations in young patients who have small vessel vasculopathy on brain imaging but who do not have significant cardiovascular risk factors.

  11. Análises dos efeitos do colágeno bovino e derivados na proliferação celular e biossíntese de colágeno em fibroblastos humanos.

    OpenAIRE

    Vergimari Rodrigues

    2009-01-01

    O colágeno é a proteína fibrosa predominante da matriz extracelular e é a maior proteína constituinte do tecido conectivo. Alterações nas taxas de colágeno tipo I na derme ocorrem durante o envelhecimento. A introdução de um agente eficaz para a manutenção da pele durante o envelhecimento é importante, possibilitando a redução destes efeitos. Neste projeto foram avaliados os efeitos proliferativos e de biossíntese de colágeno, em fibroblastos humanos de derme normal tratados com colágeno bovi...

  12. Les paradoxes du littoral Swahili

    Directory of Open Access Journals (Sweden)

    François Bart

    2008-10-01

    Full Text Available Le contact entre l'océan Indien occidental et le continent africain a forgé l'identité et l'originalité du littoral swahili. Cette interface, longue d'environ 2 000 kilomètres, du sud de la Somalie au nord du Mozambique, constitue un monde original où, au gré de traditions anciennes de circulation maritime et continentale se sont diffusées et mêlées des influences culturelles venues du continent africain, de la péninsule arabique et de la péninsule indienne. Le développement du continent, du littoral et des archipels s'inscrit dans une double logique de fragmentation et d'ouverture au monde. Dans cette vaste aire géographique, le rôle de relais des îles et des axes de pénétration continentale à partir des ports est essentiel.The identity and the original features of the Swahili seashore mainly proceed from the contact of African mainland and western indian Ocean. This coastal interface, stretching over 2 000 kilometres from southern Somalia to northern Mozambique, is characterized by ancient traditions of trade movements through maritime areas and mainland as well, which spread and mixed different cultural influences originating from Africa and both Arabic and Indian peninsulas. The development of this area is linked to both fragmentation and opening dynamics. In such a wide area, the relaying function of islands and continental corridors from main harbours is very important.

  13. Correção da magnitude da mortalidade por câncer do colo do útero no Brasil, 1996-2005 Corrección de la magnitud de la mortalidad por cáncer de colón uterino en Brasil, 1996-2005 Correction for reported cervical cancer mortality data in Brazil, 1996-2005

    Directory of Open Access Journals (Sweden)

    Carmen Justina Gamarra

    2010-08-01

    sobre los 9.607.177 óbitos fueron obtenidos del Sistema de Información sobre Mortalidad de Brasil, para el período de 1996 a 2005. Para la corrección del sub-registro, fueron utilizados los factores de expansión generados por el Proyecto Carga Global de Enfermedad en Brasil-1998. Para la corrección de las categorías de diagnósticos desconocidos, incompletos o mal definidos de óbitos, fue aplicada la metodología de redistribución proporcional. Los datos ausentes de edad fueron corregidos por imputación. Las correcciones fueron aplicadas por Unidad Federativa y los resultados son presentados para Brasil, región y áreas geográficas (capital, demás municipios de las regiones metropolitanas e interior por medio del porcentaje de variabilidad de la magnitud de las tasas, antes y después de la corrección de los óbitos. El comportamiento de las correcciones fue analizado por modelo de regresión linear multivariada con términos de interacción entre región del país y área geográfica. RESULTADOS: Las tasas corregidas de mortalidad por cáncer de colon uterino en Brasil mostraron un aumento de 103,4%, variando de 35% para las capitales de la región Sur a 339% para el interior de la región Noreste. La redistribución de los óbitos por cáncer uterino sin especificación de localización anatómica promovió los mayores aumentos en la magnitud de las tasas. Los porcentajes de corrección, según año de ocurrencia de óbito, mostraron tendencia estacionaria en Brasil. CONCLUSIONES: Los resultados permiten concluir que la metodología propuesta fue adecuada para corregir la magnitud de las tasas de mortalidad por cáncer de colon uterino en Brasil, muestreando que la mortalidad por ese cáncer es aún mayor a lo observado en los informes oficiales.OBJECTIVE: To develop a methodology for correction of reported cervical cancer deaths in Brazil. METHODS: Data on 9,607,177 cancer deaths were obtained from the Brazilian National Mortality Database for the

  14. Remedial chemo-radiotherapy for mediastinum and pleuro-pulmonary metastases of an anal canal cancer; Chimio-radiotherapie de rattrapage pour metastases mediastinales et pleuropulmonaires d'un cancer du canal anal

    Energy Technology Data Exchange (ETDEWEB)

    Moreau, M.V.; Tournier-Rangeard, L.; Peiffert, D. [Centre Alexis-Vautrin, Service de Radiotherapie, 54 - Nancy (France)

    2006-11-15

    A fifty seven years woman received ten years ago, a radiotherapy with iodine for a Basedow hyperthyroidism. In 2001 she was damaged bu an epidermoid carcinoma of the anal canal. Radiotherapy, then chemotherapy, then brachytherapy. During three years noting special to declare. In 2004, respiratory insufficiency leads her to consult. A PET scanography shows an inter-aortic-pulmonary hyper-metabolism. Cortico-therapy and radiotherapy by X rays are delivered. In front of the positive response a new chemoradiotherapy and three cycles of chemotherapy are made. After twenty five months the patient is in complete remission of her metastases after this remedial treatment. This observation illustrates the radiosensitivity of the anal canal cancers of including metastases situation, the interest of the PET scanography and utility of a remedial treatment with curative goal. (N.C.)

  15. Prognosis factors of the metastatic thyroid differentiated cancer: value of the iodo-fixation on the secondary sites; Facteurs pronostiques du cancer differencie thyroidien metastatique: valeur de la iodo-fixation sur les sites secondaires

    Energy Technology Data Exchange (ETDEWEB)

    Benisvy, D.; Benoliel, J.; Fontana, X.; Bussiere, F.; Darcourt, J. [Service de medecine nucleaire, centre Antoine-Lacassagne, Nice, (France); Chamorey, E. [unite de biostatistique, centre Antoine-Lacassagne, Nice, (France)

    2009-05-15

    The survival of patients with a differentiated thyroid cancer of follicle strain with distant metastases is very variable, going from 14 to 95% at ten year. The study of factors influencing the prognosis stays essential in order to adapt the therapy. Conclusions: According to the results published in other series, the prognosis variables were age, histology, metastases extension and the number of ira therapies. On the other hand, the existence of iodine fixation at the metastases level was not significantly correlated to a better survival. This study enlightens the importance to quantify objectively the character iodo fixing of metastases, that cannot be evaluated after resection of the remainder on a whole body scintigraphy with a strong dose. (N.C.)

  16. Conformational radiotherapy in the case of prostate cancer: experience gained by the Blida Oncology Radiotherapy Department, Algeria; La radiotherapie conformationnelle dans le cancer de la prostate: experience du service de radiotherapie oncologique de Blida, Algerie

    Energy Technology Data Exchange (ETDEWEB)

    Ayad, M.; Abbas, L.; Mesli, S.; Boualga, K. [Centre anti cancer, Blida (Algeria)

    2010-10-15

    Conformational radiotherapy is used as the first method for the treatment of localized prostate cancers. It preserves some life quality and allows a significant reduction of the dose applied to healthy organs, and therefore a drastic reduction of late and severe effects of irradiation. The authors report a prospective study which aims at describing therapeutic modalities and assessing preliminary results in terms of biochemical control and toxicity. They discuss the results obtained on 34 patients who have been treated between January 2008 and January 2009. In comparison with conventional therapy, they observe an absence of grade 3 and 4 side effects, and a normalization of biological criteria which indicates a good tumour response. Short communication

  17. Effects of Zinc Transporter 4 cDNA Transfection on Biological Behavior of Prostate Cancer Cell Line DU145%锌转运蛋白4 cDNA稳定转染对前列腺癌DU145细胞株生物学行为的影响

    Institute of Scientific and Technical Information of China (English)

    陈启光; 单广夷; 都书琪; 李振华; 孔垂泽

    2014-01-01

    目的 探讨锌转运蛋白(ZIP)4对前列腺癌细胞系生物学行为的影响.方法 比较3种不同的前列腺癌细胞系(22RVl、DU145、PC3),选择ZIP4低表达的细胞株,转染构建ZIP4过表达的前列腺癌细胞系,进一步应用MTT、Transwell及细胞划痕等试验检测ZIP4过表达后对前列腺癌细胞系增殖活性及侵袭转移能力的影响.结果 建立稳定表达ZIP4的DU 145前列腺癌细胞系;转染后的DU145-ZIP4细胞系的增殖活性及侵袭能力与转染空载体组相比均显著下降(P<0.05),但其迁移能力与转染空载体组相比未见明显变化(P>0.05).结论 ZIP4基因对前列腺癌细胞系的增殖活性及侵袭能力有显著抑制作用,ZIP4可能是前列腺癌的一个抑癌基因.

  18. Immunological characterization of recombinantWuchereria bancrofti cuticular collagen (COL-4) as putative vaccine candidate for human lymphatic filariasis

    Institute of Scientific and Technical Information of China (English)

    Chakkaravarthy Arunkumar; Pandurangan Pandiaraja; P. R. Prince; Perumal Kaliraj

    2014-01-01

    Objective:To elucidate immunoprophylactic potential of recombinantWuchereria bancrofti(W. bancrofti) cuticular collagen(COL-4) inBALB/c mice and filarial clinical samples.Methods:col-4 gene wasPCR amplified fromW. bancroftiL3 cDNA library and cloned in pRSETB vector. RecombinantCOL-4 was over expressed in salt inducible system and was purified by nickel affinity chromatography.Humoral and cellular responses were measured byELISA and peripheral blood mononuclear cells(PBMC) of various filarial clinical samples respectively using purified recombinantCOL-4 antigen.Then the protective immune responses ofCOL-4 immunizedBALB/c mice were characterized.Results:Sequence analysis ofCOL-4 with human host proteins reveals lack of homology.The recombinantCOL-4 was found to be at15 kDa fusion protein.The affinity purifiedCOL-4 showed significant reactivity with putatively immune sera and in a similar fashion it demonstrated marked proliferation inPBMC samples.Immunization studies in experimental filarial host(mice) elicited significant titers with protective antibody isotype profile(IgM and IgG).Cellular immune responses were also significant in terms of splenocytes proliferation assay on mice samples.Conclusions:Our immunological findings in experimental host suggestTh2 mediated immune response.Hence, we propose thatW. bancroftiCOL-4 could be an efficacious vaccine candidate against lymphatic filariasis.

  19. A novel COL11A1 missense mutation in siblings with non-ocular Stickler syndrome.

    Science.gov (United States)

    Kohmoto, Tomohiro; Tsuji, Atsumi; Morita, Kei-Ichi; Naruto, Takuya; Masuda, Kiyoshi; Kashimada, Kenichi; Enomoto, Keisuke; Morio, Tomohiro; Harada, Hiroyuki; Imoto, Issei

    2016-01-01

    Stickler syndrome (STL) is an autosomal, dominantly inherited, clinically variable and genetically heterogeneous connective tissue disorder characterized by ocular, auditory, orofacial and skeletal abnormalities. We conducted targeted resequencing using a next-generation sequencer for molecular diagnosis of a 2-year-old girl who was clinically suspected of having STL with Pierre Robin sequence. We detected a novel heterozygous missense mutation, NM_001854.3:n.4838G>A [NM_001854.3 (COL11A1_v001):c.4520G>A], in COL11A1, resulting in a Gly to Asp substitution at position 1507 [NM_001854.3(COL11A1_i001)] within one of the collagen-like domains of the triple helical region. The same mutation was detected in her 4-year-old brother with cleft palate and high-frequency sensorineural hearing loss.

  20. A 6.4 hr positive superhump period in TV Col

    CERN Document Server

    Retter, A

    1999-01-01

    Re-examination of photometric data of TV Col (Hellier, 1993) reveals positive superhumps in addition to the negative superhumps previously known. The superhump period is 0.265+/-0.005 day - about 16 percent longer than the orbital period - which obeys the relation between superhump-period excess and orbital period (Stolz and Schoembs 1984). As a confirmed permanent superhumper, the accretion disc of TV Col is naturally thermally stable. Therefore, our result supports the idea of Hellier and Buckley (1993) that the short-term outbursts seen in its light curve are mass transfer events rather than thermal instabilities in the disc. At 5.5-hr, TV Col has a longer orbital period than any known superhumper, and thus a mass ratio which is probably outside the range at which superhumps can occur according to current theory.

  1. Réflexion sur l’origine du processus de segmentation du marche du travail

    Directory of Open Access Journals (Sweden)

    Attia Nicole

    2006-01-01

    Full Text Available (francuski Ce travail propose une réflexion sur l'origine du processus de segmentation du marché du travail par rapport à l'entreprise. Se situe-t-elle au sein même de l'entreprise ou en amont, c'est à dire entre les entreprises? Cela revient à se demander si on peut avoir une approche microéconomique ou macroéconomique de la segmentation et, à s'interroger sur le rôle réel tenu par les firmes dans le processus. Déterminant pour la théorie, ce rôle est à repenser selon la réponse apportée à notre question.

  2. HANAC Syndrome Col4a1 Mutation Causes Neonate Glomerular Hyperpermeability and Adult Glomerulocystic Kidney Disease.

    Science.gov (United States)

    Chen, Zhiyong; Migeon, Tiffany; Verpont, Marie-Christine; Zaidan, Mohamad; Sado, Yoshikazu; Kerjaschki, Dontscho; Ronco, Pierre; Plaisier, Emmanuelle

    2016-04-01

    Hereditary angiopathy, nephropathy, aneurysms, and muscle cramps (HANAC) syndrome is an autosomal dominant syndrome caused by mutations in COL4A1 that encodes the α1 chain of collagen IV, a major component of basement membranes. Patients present with cerebral small vessel disease, retinal tortuosity, muscle cramps, and kidney disease consisting of multiple renal cysts, chronic kidney failure, and sometimes hematuria. Mutations producing HANAC syndrome localize within the integrin binding site containing CB3[IV] fragment of the COL4A1 protein. To investigate the pathophysiology of HANAC syndrome, we generated mice harboring the Col4a1 p.Gly498Val mutation identified in a family with the syndrome. Col4a1 G498V mutation resulted in delayed glomerulogenesis and podocyte differentiation without reduction of nephron number, causing albuminuria and hematuria in newborns. The glomerular defects resolved within the first month, but glomerular cysts developed in 3-month-old mutant mice. Abnormal structure of Bowman's capsule was associated with metalloproteinase induction and activation of the glomerular parietal epithelial cells that abnormally expressed CD44,α-SMA, ILK, and DDR1. Inflammatory infiltrates were observed around glomeruli and arterioles. Homozygous Col4a1 G498V mutant mice additionally showed dysmorphic papillae and urinary concentration defects. These results reveal a developmental role for the α1α1α2 collagen IV molecule in the embryonic glomerular basement membrane, affecting podocyte differentiation. The observed association between molecular alteration of the collagenous network in Bowman's capsule of the mature kidney and activation of parietal epithelial cells, matrix remodeling, and inflammation may account for glomerular cyst development and CKD in patients with COL4A1-related disorders.

  3. COL4A1 Mutations and Cerebral Small Vessel Disease%COL4A1基因突变与脑血管病

    Institute of Scientific and Technical Information of China (English)

    陈宇

    2010-01-01

    Ⅳ型胶原蛋自基因α1(αl type IV collagen,COUA1)编码IV型胶原的αl链,是构成所有组织基底膜的主要成分.COL4A1基因突变可引起脑内小动脉病变,在一定的环境压力下引发脑内小血管的阻塞或破裂,造成脑出血(intracerrbral hemorrhage,ICH).本文综述了COL4A1与α1链的结构,构成Ⅳ型胶原的方式,Ⅳ型胶原在基底膜中的作用以及目前发现的COL4A1突变引发的人类脑血管方面的疾病,并对发病机理进行了简要综述.

  4. Stem cell characteristics in prostate cancer cell lines.

    NARCIS (Netherlands)

    Pfeiffer, M.J.; Schalken, J.A.

    2010-01-01

    BACKGROUND: Recent studies indicate the presence of a small, stem-like cell population in several human cancers that is crucial for the tumour (re)population. OBJECTIVE: Six established prostate cancer (PCa) cell lines-DU145, DuCaP, LAPC-4, 22Rv1, LNCaP, and PC-3-were examined for their stem cell pr

  5. Wound healing genes and susceptibility to cutaneous leishmaniasis in Brazil: Role of COL1A1

    OpenAIRE

    Almeida, Lucas; Oliveira, Joyce; Guimarães, Luiz Henrique; Carvalho, Edgar M.; Blackwell, Jenefer M.; Castellucci, Léa

    2015-01-01

    Previous studies have demonstrated a role for wound healing genes in resolution of cutaneous lesions caused by Leishmania spp. in both mice and humans, including the gene FLI1 encoding Friend leukaemia virus integration 1. Reduction of Fli1 expression in mice has been shown to result in up-regulation of collagen type I alpha 1 (Col1a1) and alpha 2 (Col1a2) genes and, conversely, in down-regulation of the matrix metalloproteinase 1 (Mmp1) gene, suggesting that Fli1 suppression is involved in a...

  6. Anacardic acid induces apoptosis of prostate cancer Du145 cells through inhibiting expression of COX2, C-myc and VEGFA%槚如酸对雄激素非依赖型前列腺癌细胞株Du145的作用和对 COX2、C-myc、VEGFA 表达的影响及意义

    Institute of Scientific and Technical Information of China (English)

    谭靖; 蒋先镇; 汤育新; 龙智; 蒋志强; 戴英波; 何乐业

    2012-01-01

      目的探讨药物槚如酸对雄激素非依赖型前列腺癌细胞株 Du145的抗肿瘤作用及对癌基因 COX2、C-myc、VEGFA 表达的影响及意义。方法培养 Du145细胞株,使用 MTT 法测定不同浓度槚如酸对 Du145细胞增殖的影响,获得药物最佳作用时间和剂量;使用流式细胞仪检测槚如酸对 Du145细胞周期的影响和对细胞凋亡的作用;并通过 RT-PCR 及 Western blot 来测定槚如酸对 Du145细胞内癌基因 C-myc、COX2、VEGFA的 mRNA 和蛋白表达的影响。结果槚如酸抑制 Du145细胞增殖,其作用具有剂量、时间效应关系;槚如酸诱导 Du145细胞凋亡,并阻滞细胞周期于 G1/S 期;槚如酸处理后,Du145细胞株内癌基因 COX2、C-myc、VEGFA 的 mRNA 和蛋白表达均降低。结论槚如酸对雄激素非依赖型前列腺癌 Du145细胞具致死作用,其抗癌机制值得进一步探讨。%10.3969/j.issn.1008-0848.2012.12.002

  7. Cri-du-chat syndrome

    NARCIS (Netherlands)

    Didden, H.C.M.; Curfs, L.M.G

    2013-01-01

    Cri-du-chat syndrome is a genetic disease resulting from a deletion occurring on the short arm of chromosome 5 (5p-). The incidence ranges from 1:15 000 to 1:50 000 live-born infants. Its main clinical features are a high-pitched monochromatic cry, microcephaly, broad nasal bridge, epicanthal folds,

  8. A Comparative Study on Proteomics between LNCap and DU145 Cells by Quantitative Detection and SELDI Analysis

    Institute of Scientific and Technical Information of China (English)

    Weigui SUN; Zhangqun YE; Zhenguo MI; Tianliang SHI; Cunzhi HAN; Sutang GUO

    2008-01-01

    The differences in intracellular and extracellular protein expressions between human prostate cancer fines LNCap and DU145 were examined. The proteins of the two cell lines were extracted and condensed by using protein extraction kits. And the intracellular and extracellular proteins were quantitatively detected on a micro-plate reader by using bicinchoninie acid (BCA) method. The proteins in cell culture fluid were qualitatively assayed by SELDI-TOF-MS. The results showed that the intracellular protein contents of LNCap cells were extremely higher than those of DU145 cells. After serum-free culture, both intracellular and extracellular protein contents of LNCap and DU145 were decreased to some extent. And the intracellular proteins were decreased by 5% in LNCap and by 36% in DU145 respectively, while the extracellular proteins were decreased by 89% in LNCap and 96% in DU145 respectively. SELDI assay revealed that there were 5 marker proteins in LNCap and 6 in DU145. Although both LNCap and DU145 cell lines originated from human prostate cancer, they had some differences in protein expression.

  9. LES GITES PLOMBO-ZINCIFERES DU DISTRICT MINIER DU KOUDIAT SIDU AUSUD DU KEF EN TUNISIE

    Directory of Open Access Journals (Sweden)

    Ivan Jurković

    1992-12-01

    Full Text Available Les auteurs ont dćcrit l:i stratigiaphie. I.i tectonique, le corps de minerals, la paragenese et la genese dcs gttes Koudiat Sidri situ&s environ 20 km au sud de la ville du Kef en Tunisie.

  10. Cancer

    Science.gov (United States)

    Cancer begins in your cells, which are the building blocks of your body. Normally, your body forms ... be benign or malignant. Benign tumors aren't cancer while malignant ones are. Cells from malignant tumors ...

  11. Entomopathogenic fungi in predatory beetles (Col: Carabidae and Staphylinidae) from agricultural fields

    DEFF Research Database (Denmark)

    Steenberg, T; Langer, V; Esbjerg, P

    1995-01-01

    Prevalence of entomopathogenic fungi was studied in overwintering ground beetles (Col.: Carabidae) and rove beetles (Col.: Staphylinidae) collected from fields of lucerne, white cabbage and white cabbage undersown with white clover. In general infection levels in adult ground beetles and rove bee...... (Zygomycetes: Entomophthorales). Two individuals of Anotylus rugosus were found to have a dual infection of Zoophthora philonthi and Beauveria bassiana...

  12. Las cuentas del cuarto viaje de Cristóbal Colón

    Directory of Open Access Journals (Sweden)

    Gil, Juan

    2003-12-01

    Full Text Available The payroll of the fourth voyage of Christopher Columbus is here published for the fisrt time, signed by Admiral himself. A commentary is also furnished.

    Se publican por primera vez, con comentario, las cuentas del cuarto viaje de Cristóbal Colón, firmadas por el propio almirante.

  13. Fibrochondrogenesis results from mutations in the COL11A1 type XI collagen gene.

    Science.gov (United States)

    Tompson, Stuart W; Bacino, Carlos A; Safina, Nicole P; Bober, Michael B; Proud, Virginia K; Funari, Tara; Wangler, Michael F; Nevarez, Lisette; Ala-Kokko, Leena; Wilcox, William R; Eyre, David R; Krakow, Deborah; Cohn, Daniel H

    2010-11-12

    Fibrochondrogenesis is a severe, autosomal-recessive, short-limbed skeletal dysplasia. In a single case of fibrochondrogenesis, whole-genome SNP genotyping identified unknown ancestral consanguinity by detecting three autozygous regions. Because of the predominantly skeletal nature of the phenotype, the 389 genes localized to the autozygous intervals were prioritized for mutation analysis by correlation of their expression with known cartilage-selective genes via the UCLA Gene Expression Tool, UGET. The gene encoding the α1 chain of type XI collagen (COL11A1) was the only cartilage-selective gene among the three candidate intervals. Sequence analysis of COL11A1 in two genetically independent fibrochondrogenesis cases demonstrated that each was a compound heterozygote for a loss-of-function mutation on one allele and a mutation predicting substitution for a conserved triple-helical glycine residue on the other. The parents who were carriers of missense mutations had myopia. Early-onset hearing loss was noted in both parents who carried a loss-of-function allele, suggesting COL11A1 as a locus for mild, dominantly inherited hearing loss. These findings identify COL11A1 as a locus for fibrochondrogenesis and indicate that there might be phenotypic manifestations among carriers.

  14. Stimulation of Escherichia coli F-18Col- Type-1 fimbriae synthesis by leuX

    DEFF Research Database (Denmark)

    Newman, Joseph V.; Burghoff, Robert L.; Pallesen, Lars

    1994-01-01

    alone but is eliminated when fed together with E. coli F-18. Recently we randomly cloned E. coli F-18 DNA into E. coli F-18Col- and let the mouse intestine select the best colonizer. In this way, we isolated a 6.5-kb E. coli F-18 DNA sequence that simultaneously stimulated synthesis of type 1 fimbriae...

  15. Phase I and pharmacokinetic study of COL-3 in patients with recurrent high-grade gliomas.

    Science.gov (United States)

    Rudek, Michelle A; New, Pamela; Mikkelsen, Tom; Phuphanich, Surasak; Alavi, Jane B; Nabors, Louis B; Piantadosi, Steven; Fisher, Joy D; Grossman, Stuart A

    2011-11-01

    COL-3 is a chemically modified tetracycline that targets multiple aspects of matrix metalloproteinase regulation. This phase I clinical trial was conducted to determine the maximum tolerated dose (MTD) of COL-3 in adults with recurrent high-grade glioma, to describe the effects of enzyme-inducing antiseizure drugs (EIADs) on its pharmacokinetics, and to obtain preliminary evidence of activity. Adults with recurrent high-grade glioma were stratified by EIAD use. COL-3 was given orally daily without interruption until disease progression or treatment-related dose-limiting toxicity (DLT). Three patients in each EIAD group were evaluated at each dose level beginning with 25 mg/m(2)/day and escalated by 25 mg/m(2)/day. Toxicity, response, and pharmacokinetics were assessed. Thirty-three patients were evaluated. The MTD was 75 mg/m(2)/day in the -EIAD patients while one was not determined in +EIAD patients. The common toxicities observed were anemia, ataxia, diarrhea, hypokalemia, CNS hemorrhage, and myalgia. One partial response was observed. -EIAD patients tended to have a higher steady-state trough concentration that was apparent only at the 100 mg/m(2)/day dose level (P = 0.01). This study suggests that: (a) EIAD use does affect the pharmacokinetics of COL-3 at higher doses; and (b) there was not enough suggestion of single-agent activity to warrant further study in recurrent high-grade gliomas.

  16. Two-step method for curing Escherichia coli of ColE1-derived plasmids

    DEFF Research Database (Denmark)

    Hove-Jensen, Bjarne

    2008-01-01

    To cure Escherichia coli for plasmids derived from the ColE1 replicon advantage is taken of the fact that maintenance of this replicon requires a wild-type allele of polA, encoding DNA polymerase I. Curing is achieved by cotransduction of a mutant polA allele with metE::Tn10, fadAB::Tn10 or other...

  17. Effects of a malfunctional column on conventional and FeedCol-simulated moving bed chromatography performance.

    Science.gov (United States)

    Song, Ji-Yeon; Oh, Donghoon; Lee, Chang-Ha

    2015-07-17

    The effects of a malfunctional column on the performance of a simulated moving bed (SMB) process were studied experimentally and theoretically. The experimental results of conventional four-zone SMB (2-2-2-2 configuration) and FeedCol operation (2-2-2-2 configuration with one feed column) with one malfunctional column were compared with simulation results of the corresponding SMB processes with a normal column configuration. The malfunctional column in SMB processes significantly deteriorated raffinate purity. However, the extract purity was equivalent or slightly improved compared with the corresponding normal SMB operation because the complete separation zone of the malfunctional column moved to a lower flow rate range in zones II and III. With the malfunctional column configuration, FeedCol operation gave better experimental performance (up to 7%) than conventional SMB operation because controlling product purity with FeedCol operation was more flexible through the use of two additional operating variables, injection time and injection length. Thus, compared with conventional SMB separation, extract with equivalent or slightly better purity could be produced from FeedCol operation even with a malfunctional column, while minimizing the decrease in raffinate purity (less than 2%).

  18. Evidence for large superhumps in TX Col and V4742 Sgr

    CERN Document Server

    Retter, A; Liu, A; Bos, Marc; Liu, Alexander; Retter, Alon

    2004-01-01

    Since the discovery of the largest positive superhump period in TV Col, we have started a program to search for superhumps in CVs with large orbital periods. Here, we summarize preliminary results of TX Col and V4742 Sgr. TX Col is an intermediate polar with a 5.7-h orbital period. V4742 Sgr is a recent nova with no known periods. CCD unfiltered continuous photometry of these 2 objects was carried out during 56 nights in 2002-3. In TX Col, in addition to the orbital period of 5.7 h, we found peaks at 7.1 h and 5.0 h. These are interpreted as positive and negative superhumps correspondingly, although the effects of the quasi-periodic oscillations at about 2 h were not taken into consideration. In the light curve of V4742 Sgr 2 long periods are detected -- 6.1 and 5.4 h as well as a short-term period at 1.6 h. This result suggests that V4742 Sgr is an intermediate polar candidate and a permanent superhump system with a large orbital period (5.4 h) and a superhump period excess of 13 percent. If these results ar...

  19. Effects of clinorotation on COL1A1- EGFP gene expression

    Institute of Scientific and Technical Information of China (English)

    DAI Zhongquan; LI Yinghui; DING Bai; ZHANG Yuguo; LIU Weiquan; LIU Pengpeng

    2004-01-01

    Bone-formation related gene plays a critical role in bone loss induced by space microgravity, however the exact mechanism is unclear. In this study, we aim to investigate the effect of microgravity on the activity of α 1(I) collagen (COL1A1) gene promoter and the expression of osteoblast-related genes. COL1A1 promoter was digested by restriction enzymes resulting in three DNA fragments. The fragments were ligated with the enhanced green fluorescent protein report gene, and subcloned into expression vectors. ROS17/2.8 cells transfected by these vectors were screened by G418, and enhanced green fluorescent protein (EGFP) positive colonies were isolated and cultured under clinostat condition. EGFP and Collagen type I expression level were detected by fluorescence intensity analysis and immunocytochemistry methods respectively. The results showed that the expression of EGFP and collagen type I was increased 24 h, 48 h after the cells were cultured under stimulated microgravity, illustrating that the activity of COL1A1 promoter might be increased. In conclusion, osteoblasts can compensatively increase the expression of type I collagen by enhancing the activity of COL1A1 promoter under short-term simulated microgravity conditions.

  20. Dynamique narrative du texte, du film et de la musique

    OpenAIRE

    Wildgen, Wolfgang

    2015-01-01

    La narrativité est foncièrement liée à la dynamique des événements et des actions représentés, et elle dépend du champ pragmatique narrateur/récepteur, c’est-à-dire du discours narratif. Cette dynamique demande une théorisation adéquate, par exemple au sein de la théorie des systèmes dynamiques ou de l’analyse vectorielle, et elle se manifeste dans des modalités différentes. Nous présentons des exemples d’analyse dans la modalité linguistique (récit oral spontané, conte populaire), dans la mo...

  1. Le parcours migratoire de jeunes ruraux du bled du kif

    Directory of Open Access Journals (Sweden)

    Khalid Mouna

    2015-06-01

    Full Text Available Cet article analyse le parcours migratoire des jeunes ruraux originaires des zones de production du cannabis, jeunes qui cherchent à briser les chaînes de soumission et d’humiliation vécues au quotidien. Pour les jeunes concernés par notre étude, la migration constitue un moyen de s’intégrer dans des réseaux transnationaux et ainsi d’entamer une carrière de beznass (commerçant du cannabis. Ce parcours « initiatique » permet à ces jeunes de revenir au bled avec de nouvelles idées, des moyens accrus, et de jouer un rôle actif dans l’économie locale – qui reste pour eux focalisée sur la production de cannabis, cette dernière restant néanmoins officiellement interdite.

  2. Role and mechanism of Twist1 in modulating the chemosensitivity of FaDu cells.

    Science.gov (United States)

    Lu, Sumei; Yu, Liang; Mu, Yakui; Ma, Juke; Tian, Jiajun; Xu, Wei; Wang, Haibo

    2014-07-01

    Multidrug resistance (MDR) is one of the most important obstacles affecting the efficacy of chemotherapy treatments for numerous types of cancer. In the present study, we have demonstrated the possible function of Twist1 in the chemosensitivity of head and neck squamous cell carcinoma (HNSCC) and have identified that its mechanism maybe associated with MDR1/P-gp regulation. To investigate this, the hypopharyngeal cancer cell line, FaDu, and its MDR cell line induced by taxol, FaDu/T, were employed. Stable transfectants targeted to Twist1 overexpression and Twist1 silencing based on FaDu were also conducted. Morphological observation, flow cytometry, reverse transcription-polymerase chain reaction (RT-PCR), western blotting and laser scanning confocal microscope detection were utilized to detect the associations between Twist1 and the chemosensitivity of FaDu cells. Our results demonstrated that Twist1 and MDR1/P-gp were upregulated in FaDu/T cells in a MDR dose-dependent manner. The anti-apoptotic capabilities of FaDu/T cells were enhanced during MDR progression, with apoptosis-related proteins (Bcl-2, Bax, activated caspase-3 and caspase-9) changing to resist apoptosis. Twist1 overexpression decreased the sensitivity of cells to taxol as revealed by a significant increase in MDR1/P-gp and IC50 (Pcell death, and inhibited Ca2+ release induced by taxol (Pcells also confirmed this result. This study provided evidence that alterations of Twist1 expression modulates the chemosensitivity of FaDu cells to taxol. Therefore, Twist1 knockdown may be a promising treatment regimen for advanced hypopharyngeal carcinoma patients with MDR.

  3. NOVEL SPLICING MUTATION OF COL1A1 GENE CAUSING OSTEOGENESIS IMPERFECTA TYPE I IN CHINESE PEDIGREE

    Institute of Scientific and Technical Information of China (English)

    WU Xiao-lin; GU Ming-min; CUI Bing; LI Xi-hua; LU Zhen-yu; WANG Zhu-gang; YUAN Wen-tao; SONG Huai-dong

    2007-01-01

    Objective To detect the peculiar mutation in a Chinese family with osteogenesis imperfecta,COL1A1 and COL1A2 being analysed. Methods A genome screen was undertaken covering COL1A1 at 17q21-22 and COL1A2 at 7q22.1. The Linkage ( Version 5.1 ) was used for 2-point analysis. DNA sequencing was used to screen and identify the mutation. Results A linkage to the markers on chromosome 17q21-22 was observed. Sequence analysis of COL1A1 revealed a splicing mutation ( IVS8-2A > G) that converted the 3' end of intron 8 from AG to GG. Conclusion This mutation ( IVS 8-2A > G) is novel, and has not yet been registered in the Human Type Ⅰ and Type Ⅲ Collagen Mutations Database.

  4. Col-F, a fluorescent probe for ex vivo confocal imaging of collagen and elastin in animal tissues.

    Science.gov (United States)

    Biela, Ewa; Galas, Jerzy; Lee, Brian; Johnson, Gary L; Darzynkiewicz, Zbigniew; Dobrucki, Jurek W

    2013-06-01

    A new low-molecular-weight fluorescent probe, Col-F, that exhibits affinity to collagen and elastin, was used successfully in imaging of extracellular matrix in freshly excised animal tissues. Col-F readily penetrates between live cells into tissues and binds to fibers of collagen and elastin by a noncovalent mechanism. Fibers of collagen and elastin have been stained in a variety of tissues, including tendon, skeletal muscle, connective tissue, and arteries. Cells migrating in a Col-F-stained collagenous biomaterial were also imaged. No phototoxic effects were detected when live keratocytes were imaged in the in vitro culture in the presence of Col-F. In conclusion, Col-F provides a simple and convenient tool for fluorescence three-dimensional imaging of intricate collagenous and elastic structures in live and fixed animal tissues, as well as in collagen-containing biomaterials.

  5. L’Harmonie du monde

    Directory of Open Access Journals (Sweden)

    Martine Clouzot

    2003-11-01

    Full Text Available La Bourgogne est particulièrement bien présente et représentée dans l’exposition sur la musique et ses représentations au Moyen Âge organisée par Isabelle Marchesin (université de Poitiers, Christine Laloue (conservatrice du Patrimoine au Musée et Martine Clouzot (université de Bourgogne, au Musée de la Musique à Paris du 26 mars au 27 juin 2004. En Côte-d’Or, à Dijon, la Bibliothèque municipale a donné son accord officiel pour le prêt de la Bible d’Etienne Harding, les Moralia in Job et u...

  6. Evaluation of the cytotoxic effects of PLGA coated iron oxide nanoparticles as a carrier of 5- fluorouracil and mega-voltage X-ray radiation in DU145 prostate cancer cell line.

    Science.gov (United States)

    Hajikarimi, Zahra; Khoei, Samideh; Khoee, Sepideh; Mahdavi, Seied Rabi

    2014-12-01

    The purpose of this study was to investigate the uptake and cytotoxic effects of magnetic poly lactic-co-glycolic acid (PLGA)-coated iron oxide nanoparticles as a carrier of 5-fluorouracil (5-FU) and X-ray on the level of proliferation capacity of DU145 prostate carcinoma cell line in monolayer culture. Following monolayer culture, DU 145 cells were treated with different concentrations of 5-FU or 5-FU loaded nanoparticles for 24 h and 2Gy X-ray (6 Mega-voltage (MV)). The rate of nanoparticles penetration was then measured using atomic adsorption spectroscopy (AAS). The cytotoxicity effect of these nanoparticles with/ without X-ray radiation was evaluated using colony formation assay. Spectroscopy results showed that iron content and therefore the cellular uptake of 5-FU loaded nanoparticles increased with increasing nanoparticle concentrations. Further, the proliferation capacity of the cells decreased with the increase of 5-FU and 5- FU loaded nanoparticle concentrations in combination with X-ray radiation. However the extent of reduction in colony number following treatment with 5-FU-loaded nanoparticles in combination with 2Gy of megavoltage X-ray radiation was significantly more than for free 5-FU. Thus, drug-loaded nanoparticles could deliver 5-FU more efficiently into the cells. PLGA coated iron oxide nanoparticles are therefore effective drug delivery vehicles for 5-FU. PLGA coated iron oxide nanoparticles are biocompatible and this coating is an appropriate surface that can penetrate into the cells.

  7. L'energie du moustique

    CERN Document Server

    Augereau, J F

    2002-01-01

    ENSEMBLE DE QUATRE ARTICLES - LARGE HADRON COLLIDER: Le dernier accelerateur de particules du CERN, le LEP, produisait des faisceaux d'electrons de 100 GeV chacun. Le LHC, qui accelere des faisceaux de protons, leur communiquera une energie de 7 TeV chacun. Une energie a la fois colossale et derisoire. Un TeV represente a peu pres l'energie cinetique d'un moustique (1/2 page).

  8. Skeletal phenotypes in adult patients with osteogenesis imperfecta-correlations with COL1A1/COL1A2 genotype and collagen structure

    DEFF Research Database (Denmark)

    Hald, J D; Folkestad, L; Harsløf, T

    2016-01-01

    Osteogenesis imperfecta (OI) is characterized by a high fracture rate and great heterogeneity. This cross-sectional study presents skeletal investigations and protein analyses in 85 adult OI patients. We find significant differences in bone mass, architecture, and fracture rate that correlate well...... of this study was to improve our understanding of clinical manifestations by investigating anthropometry and skeletal phenotypes (DXA, HRpQCT) in an adult OI population and compare the findings to underlying COL-1 genotype and structure. METHODS: The study comprised 85 OI patients aged 45 (19-78) years...

  9. Prairie du Chien: A Historical Study,

    Science.gov (United States)

    1976-10-01

    Naudouwessee with whom this governor made peace and introduced trade and commerce among the whole nation. His son the successor told me that some of the...Nicholas Perrot, French Commandant of the West, with a party of couriers du bois and voyageurs descended the Wisconsin River and erected Fort St...number of traders and voyageurs settled at Prairie du Chien. Thomas Anderson who settled at Prairie du Chien in 1800 described the conditions on his

  10. Genetics Home Reference: cri-du-chat syndrome

    Science.gov (United States)

    ... Health Conditions cri-du-chat syndrome cri-du-chat syndrome Enable Javascript to view the expand/collapse ... PDF Open All Close All Description Cri-du-chat (cat's cry) syndrome, also known as 5p- (5p ...

  11. Effects of Different Exercise Modes on the MHC- Ⅱ, COL- I and COL-Ⅲ in Healing Process of Acute Skeletal Muscle Injury of Rats%不同运动方式对大鼠骨骼肌急性拉伤修复过程中MHC-Ⅱ,COL-Ⅰ、COL-Ⅲ的影响

    Institute of Scientific and Technical Information of China (English)

    廖远朋; 矫玮

    2011-01-01

    Purpose To investigate the effect of eccentric and concentric exercises on the fibrosis and the regeneration in acute skeletal muscle sprain healing process. Methods One hundred and twenty adult female SD rats were divided randomly into 6 groups: blank control group (BC, ? = 8) , immediate after acute sprain group (IM? N = 8) , first week after acute sprain group (1W? N = 8) , natural-healing group (NH, n = 32) , concentric-exercise group (CE, n = 32) and eccentric-exercise group (EE, n = 32) . One week after the acute muscle sprain, the CE group and EE group were respectively carried on concentric exercise and eccentric exercise for a total of 4 weeks. Gastrocnemius inIM group. 1W group, and BC group was drawn immediate. 7 days and 18 days after the acute muscle sprain, and in the others was drawn 14 days, 21 days, 28 days and 35 days after the acute muscle sprain, respectively. The MHC-II (myosin heavy chain-II) , and collagen type I (COL-1 ) and type III (COL-HI) were detected through immunohistochemistry. Results The concentric exercise accelerated the synthesis of MHC- II and the eccentric exercise accelerated the synthesis of COL-M in the whole process of healing, especially in early stage; both of the eccentric and concentric exercise decreased COL-1. Conclusion The concentric exercise promotes and accelerates the regeneration process by increasing the MHC- II; and both of them inhibit the fibrosis process, especially the concentric exercise. Eccentric exercises endows the injured muscle with better strength and mechanical stability in early stage and promotes the fibrosis process in the later stage of the healing process by increasing COL- M.%目的:了解不同运动方式(离心运动、向心运动)对骨骼肌拉伤愈合过程中肌肉再生和纤维化过程的影响.方法:将120只成年雌性SD大鼠随机分为空白组(BC组,n=8)、即刻组(IM组,n=8)、第1周组(1W组,n=8)、自然愈合组(NC组,n=32)、向心运动组(CE组,n=32)

  12. Au fil du temps (1976 ou la loi du seuil

    Directory of Open Access Journals (Sweden)

    Isabelle Singer

    2010-04-01

    Full Text Available Ayant choisi de vivre dans un camion, les héros d’Au fil du temps (film de Wim Wenders de 1976 font du seuil une expérience particulière. Celle de la rupture avec toute idée de foyer et celle du refus d’appartenance à la terre natale, cette terre allemande traversée par une frontière le long de laquelle ils vont voyager, et qui les renvoie à chaque instant aux traumatismes de l’Histoire. Sur le pare-brise du camion, l’extérieur (campagnes indifférenciées, villes à l’abandon… et l’intérieur se superposent. Au fil du temps questionne le paysage : il s’agit d’en décoller un à un les mythes qui le recouvrent. L’image alors n’est plus surface mais volume à traverser, à lacérer et découvrir ce qui est tissé dans le paysage. La démarche de Wenders est alors proche de celle d’un de ses contemporains : Anselm Kiefer. Le paysage allemand provoque le rejet parce qu’il y a là toujours plus que le visible : des strates et des strates de culpabilité que le mythe - et c’est sa fonction - a recouvert. Et qu’il s’agira ici, de soulever. Le choix du nomadisme, c’est celui d’un état de l’humanité antérieur à l’idée de patrie. Et c’est aussi celui de la solitude, comme prix à payer à ce refus d’appartenance et à cette mise à jour des mythes. L’appartenance à la terre allemande et à son Histoire est profondément problématique parce que les pères sont fondamentalement coupables. On se reconnaîtra alors des pères de substitution : des pères de cinéma (Nicholas Ray ou Fritz Lang. Et l’on substituera l’Histoire du cinéma à l’Histoire. Bruno est réparateur ambulant de projecteurs et Au fil du temps dressera, au gré de ses pérégrinations, un état des lieux du cinéma allemand des années soixante-dix : déliquescent, colonisé par les images hollywoodiennes. Il faut que cela change : état du cinéma ; état des protagonistes solitaires en quête d’une identit

  13. High mutation detection rate in the COL4A5 collagen gene in suspected Alport syndrome using PCR and direct DNA sequencing

    DEFF Research Database (Denmark)

    Martin, P; Heiskari, N; Zhou, J

    1998-01-01

    Approximately 85% of patients with Alport syndrome (hereditary nephritis) have been estimated to have mutations in the X chromosomal COL4A5 collagen gene; the remaining cases are autosomal with mutations in the COL4A3 or COL4A4 genes located on chromosome 2. In the present work, the promoter...

  14. COL1A1/COL1A2基因突变分析与成骨不全的产前基因诊断%Gene mutation analysis of COL1A1/COL1A2 and prenatal genetic diagnosis of osteogenesis imperfecta

    Institute of Scientific and Technical Information of China (English)

    李焕铮; 唐少华; 毛义建; 谢丙乐

    2012-01-01

    Objective: In this study, the patients with OI pregnancy history were required to have ultrasound ( US) examination and COL1A1/COL1A2 gene mutation detection. It is helpful to establish a clinical protocol for OI patient and provide the technical security for prenatal diagnosis. Methods: The pregnant woman with Oi pregnancy history must be monitored by US. According to ultra-sonography of fetal femur and long bone, it was diagnosed as OI. Amniotic fluid was drawn from pregnant woman with ultrasound abnormality. The sample was detected for COL1A1 and C0L1A2 mutations using direct sequencing. According to the result of new mutation detection, the COL1A1 and C0L1A2 genetic mutation for all family members were detected and analyzed. Identification of maternal blood contamination must be done before tests. Results; The ultrasound imaging showed that the fetus' femur was short, tibiofibula was angled bend, skull was thinning and multiple fractures were found. So we consider fetus was OI. The sample was identified without maternal blood contamination by STR. The COL1A1 of fetus was identified with 19 SNP loci, but no mutation was found. The C0L1A2 was identified with 13 SNP loci, and a G and A heterozygosis mutation at the exon 36 was found. The pregnant woman was found with the same mutation, but the clinical picture was different. Other members were tested with no detectable mutation. Conclusion; The pregnant woman with OI pregnancy history would be monitored by US and detected by genetic tests. It is very import for the sake of birth defects prevention.%目的 对有成骨不全(Osteogenesis Imperfecta,OI)孕史的患者,进行系统B超及COLtA1/COL1A2基因检测,希望建立OI患儿产前诊断方案,为OI患儿进行产前诊断提供技术保障.方法 对于有OI孕史的孕妇,进行系统B超监测;根据胎儿股骨、长骨的超声影像学表现,初诊为成骨不全.抽取羊水,采用直接测序法对羊水DNA的COL1A1和COL1A2基因全编码外显子

  15. Porencephaly in a fetus and HANAC in her father: variable expression of COL4A1 mutation.

    Science.gov (United States)

    Takenouchi, Toshiki; Ohyagi, Masaki; Torii, Chiharu; Kosaki, Rika; Takahashi, Takao; Kosaki, Kenjiro

    2015-01-01

    COL4A1-associated disorders encompass a wide range of hereditary vasculopathy, including porencephaly and HANAC (adult-onset hemorrhagic stroke with cerebral aneurysm and retinal arterial tortuosity, renal cysts, and thenar muscle cramp). It remains elusive whether or not porencephaly and HANAC are molecularly distinctive disorders due to different classes of mutations. We report on a girl with porencephaly and an episode of microangiopathic hemolysis in infancy and her father with HANAC, both of whom had a heterozygous missense mutation of COL4A1 (c.3715G>A, p.G1239R). The current observation implies phenotypic diversities of COL4A1 mutations.

  16. Explorant el treball a la blogosfera: la paradoxa de l’aprenentatge col·laboratiu

    Directory of Open Access Journals (Sweden)

    Jorge Lizandra Mora

    2016-06-01

    Full Text Available Amb l’objectiu de solucionar diversos problemes que sorgiren en la posada en pràctica d’una experiència d’innovació basada en la creació d’una blogosfera, es portà a terme una investigació educativa i qualitativa a partir de la col·laboració docent i de les opinions de l’alumnat. A més dels aspectes positius, es detectaren dificultats amb la gestió del treball en equipo i de familiarització amb la ferramenta a les que els propis estudiants proposaren diverses solucions. Abordar aquesta circumstància de forma col·laborativa i donant veu a l’alumnat ha permès que l’experiència concloguera exitosament, resultant ser una bona pràctica educativa.

  17. Structural constraints on the evolution of the collagen fibril: convergence on a 1014-residue COL domain.

    Science.gov (United States)

    Slatter, David Anthony; Farndale, Richard William

    2015-05-01

    Type I collagen is the fundamental component of the extracellular matrix. Its α1 gene is the direct descendant of ancestral fibrillar collagen and contains 57 exons encoding the rod-like triple-helical COL domain. We trace the evolution of the COL domain from a primordial collagen 18 residues in length to its present 1014 residues, the limit of its possible length. In order to maintain and improve the essential structural features of collagen during evolution, exons can be added or extended only in permitted, non-random increments that preserve the position of spatially sensitive cross-linkage sites. Such sites cannot be maintained unless the twist of the triple helix is close to 30 amino acids per turn. Inspection of the gene structure of other long structural proteins, fibronectin and titin, suggests that their evolution might have been subject to similar constraints.

  18. Somatic mosaicism and the phenotypic expression of COL2A1 mutations.

    Science.gov (United States)

    Nagendran, Sonali; Richards, Allan J; McNinch, Annie; Sandford, Richard N; Snead, Martin P

    2012-05-01

    Mutations in COL2A1, the gene for type II-collagen, can result in a wide variety of phenotypes depending upon the nature of the mutation. Dominant negative mutations tend to result in severe and often lethal skeletal dysplasias such as achondrogenesis type 2, Kniest dysplasia, and spondyloepiphyseal dysplasia congenita. Stickler syndrome, a condition characterized by ophthalmological and orofacial features, deafness and arthritis, usually, but not exclusively, results from haploinsufficiency. Overlapping features of all these disorders can also be seen in the same family. Rare reports have demonstrated that phenotypic variability can be explained in some families by somatic mosaicism. Here, we describe five further examples of somatic mosaicism of COL2A1 mutations illustrating the importance of detailed clinical evaluation and molecular testing even in clinically normal parents of affected individuals.

  19. Estudio del proceso de transformación del colágeno en gelatina

    OpenAIRE

    Bonmatí Limorte, María del Carmen; León Albert, Gerardo

    1983-01-01

    Se ha realizado un estudio del proceso de transformación de colágeno en gelatina utilizando la viscosidad reducida como parámetro de control. Se ha observado una gran influencia de la temperatura y del tiempo de calentamiento sobre este proceso, así como sobre la temperatura de desnaturalización. Se han determinado igualmente las entalpia y entropía de desnaturalización.

  20. Views of Astronaut (Col.) Joe Engle and son Jon with L-5 Piper Cub

    Science.gov (United States)

    1981-01-01

    Views of Astronaut (Col.) Joe Engle and son Jon with L-5 Piper Cub at Clover Airport. Photos includes Jon Engle sitting on side door frame working on portion of wing. Joe Engle is behind him working on a wing strut (34329); Joe Engle works on tightening bolt (34330); Jon Engle works on portion of wing which connects to the cockpit. Joe Engle works on connecting strut to wing (34331).

  1. Colômbia 2010: Guerra, Paz e Eleições

    Directory of Open Access Journals (Sweden)

    Luiz Antônio Gusmão

    2009-02-01

    Full Text Available

    O enfraquecimento das Farc deve polarizar as

    eleições de 2010 na Colômbia entre recrudescimento

    do combate e abertura à negociação.

  2. Session du Conseil du CERN : le ministre britannique, Robert Jackson, souligne l'intérêt de on pays pour l'avenir du CERN : décisions du Conseil pour la mise en oeuvre des recommandations du Comité d'évaluation du CERN: départ anticipé pour 200 membres au moins du personnel - mise à jour de la méthode de calcule pour les contributions des Etats Membres au budget

    CERN Multimedia

    CERN Press Office. Geneva

    1988-01-01

    Session du Conseil du CERN : le ministre britannique, Robert Jackson, souligne l'intérêt de on pays pour l'avenir du CERN : décisions du Conseil pour la mise en oeuvre des recommandations du Comité d'évaluation du CERN: départ anticipé pour 200 membres au moins du personnel - mise à jour de la méthode de calcule pour les contributions des Etats Membres au budget

  3. Synergistic interaction of platelet derived growth factor (PDGF) with the surface of PLLA/Col/HA and PLLA/HA scaffolds produces rapid osteogenic differentiation.

    Science.gov (United States)

    Raghavendran, Hanumantha Rao Balaji; Mohan, Saktiswaren; Genasan, Krishnamurithy; Murali, Malliga Raman; Naveen, Sangeetha Vasudevaraj; Talebian, Sepehr; McKean, Robert; Kamarul, Tunku

    2016-03-01

    Scaffolds with structural features similar to the extracellular matrix stimulate rapid osteogenic differentiation in favorable microenvironment and with growth factor supplementation. In this study, the osteogenic potential of electrospun poly-l-lactide/hydroxyapatite/collagen (PLLA/Col/HA, PLLA/HA and PLLA/Col) scaffolds were tested in vitro with the supplementation of platelet derived growth factor-BB (PDGF-BB). Cell attachment and topography, mineralization, extracellular matrix protein localization, and gene expression of the human mesenchymal stromal cells were compared between the fibrous scaffolds PLLA/Col/HA, PLLA/Col, and PLLA/HA. The levels of osteocalcin, calcium, and mineralization were significantly greater in the PLLA/Col/HA and PLLA/HA compared with PLLA/Col. High expression of fibronectin, intracellular adhesion molecule, cadherin, and collagen 1 (Col1) suggests that PLLA/Col/HA and PLLA/HA scaffolds had superior osteoinductivity than PLLA/Col. Additionally, osteopontin, osteocalcin, osterix, Runt-related transcription factor 2 (Runx2), and bone morphogenic protein (BMP2) expression were higher in PLLA/Col/HA and PLLA/HA compared with PLLA/Col. In comparison with PLLA/Col, the PLLA/Col/HA and PLLA/HA scaffolds presented a significant upregulation of the genes Runx2, Col 1, Integrin, osteonectin (ON), bone gamma-carboxyglutamic acid-containing protein (BGALP), osteopontin (OPN), and BMP2. The upregulation of these genes was further increased with PDGF-BB supplementation. These results show that PDGF-BB acts synergistically with PLLA/Col/HA and PLLA/HA to enhance the osteogenic differentiation potential. Therefore, this combination can be used for the rapid expansion of bone marrow stromal cells into bone-forming cells for tissue engineering.

  4. Des Taiwanais dans Ie sud du Fujian

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Se trouvant dans le sud-est de la Chine au bord dela mer, la province du Fujian s’appelle aussi toutsimplement "Min". Les anc(?)tres d’une grande partie de lapopulation de Taiwan sont venus du Fujian (Min). Les deux

  5. Generation of Col2a1-EGFP iPS cells for monitoring chondrogenic differentiation.

    Directory of Open Access Journals (Sweden)

    Taku Saito

    Full Text Available Induced pluripotent stem cells (iPSC are a promising cell source for cartilage regenerative medicine; however, the methods for chondrocyte induction from iPSC are currently developing and not yet sufficient for clinical application. Here, we report the establishment of a fluorescent indicator system for monitoring chondrogenic differentiation from iPSC to simplify screening for effective factors that induce chondrocytes from iPSC. We generated iPSC from embryonic fibroblasts of Col2a1-EGFP transgenic mice by retroviral transduction of Oct4, Sox2, Klf4, and c-Myc. Among the 30 clones of Col2a1-EGFP iPSC we established, two clones showed high expression levels of embryonic stem cell (ESC marker genes, similar to control ESC. A teratoma formation assay showed that the two clones were pluripotent and differentiated into cell types from all three germ layers. The fluorescent signal was observed during chondrogenic differentiation of the two clones concomitant with the increase in chondrocyte marker expression. In conclusion, Col2a1-EGFP iPSC are useful for monitoring chondrogenic differentiation and will contribute to research in cartilage regenerative medicine.

  6. A COL4A5 mutation with glomerular disease and signs of chronic thrombotic microangiopathy.

    Science.gov (United States)

    Wuttke, Matthias; Seidl, Maximilian; Malinoc, Angelica; Prischl, Friedrich C; Kuehn, E Wolfgang; Walz, Gerd; Köttgen, Anna

    2015-12-01

    COL4A5 mutations are a known cause of Alport syndrome, which typically manifests with haematuria, hearing loss and ocular symptoms. Here we report on a 16-year-old male patient with a negative family history who presented with proteinuria, progressive renal failure and haemolysis, but without overt haematuria or hearing loss. A renal biopsy revealed features of atypical IgA nephropathy, while a second biopsy a year later showed features of focal segmental glomerulosclerosis, but was finally diagnosed as chronic thrombotic microangiopathy. Targeted sequencing of candidate genes for steroid-resistant nephrotic syndrome and congenital thrombotic microangiopathy was negative. Despite all therapeutic efforts, including angiotensin-converting enzyme inhibition, immunosuppressive therapy, plasma exchanges and rituximab, the patient progressed to end-stage renal disease. When a male cousin presented with nephrotic syndrome years later, whole-exome sequencing identified a shared disruptive COL4A5 mutation (p.F222C) that showed X-linked segregation. Thus, mutations in COL4A5 give rise to a broader spectrum of clinical presentation than commonly suspected, highlighting the benefits of comprehensive rather than candidate genetic testing in young patients with otherwise unexplained glomerular disease. Our results are in line with an increasing number of atypical presentations of single-gene disorders identified through genome-wide sequencing.

  7. Loss of col8a1a function during zebrafish embryogenesis results in congenital vertebral malformations.

    Science.gov (United States)

    Gray, Ryan S; Wilm, Thomas P; Smith, Jeff; Bagnat, Michel; Dale, Rodney M; Topczewski, Jacek; Johnson, Stephen L; Solnica-Krezel, Lilianna

    2014-02-01

    Congenital vertebral malformations (CVM) occur in 1 in 1000 live births and in many cases can cause spinal deformities, such as scoliosis, and result in disability and distress of affected individuals. Many severe forms of the disease, such as spondylocostal dystostosis, are recessive monogenic traits affecting somitogenesis, however the etiologies of the majority of CVM cases remain undetermined. Here we demonstrate that morphological defects of the notochord in zebrafish can generate congenital-type spine defects. We characterize three recessive zebrafish leviathan/col8a1a mutant alleles ((m531, vu41, vu105)) that disrupt collagen type VIII alpha1a (col8a1a), and cause folding of the embryonic notochord and consequently adult vertebral column malformations. Furthermore, we provide evidence that a transient loss of col8a1a function or inhibition of Lysyl oxidases with drugs during embryogenesis was sufficient to generate vertebral fusions and scoliosis in the adult spine. Using periodic imaging of individual zebrafish, we correlate focal notochord defects of the embryo with vertebral malformations (VM) in the adult. Finally, we show that bends and kinks in the notochord can lead to aberrant apposition of osteoblasts normally confined to well-segmented areas of the developing vertebral bodies. Our results afford a novel mechanism for the formation of VM, independent of defects of somitogenesis, resulting from aberrant bone deposition at regions of misshapen notochord tissue.

  8. Image du savoir, image du pouvoir dans le Lapidaire

    Directory of Open Access Journals (Sweden)

    Marta LACOMBA

    2007-01-01

    Full Text Available À travers le prologue et les enluminures de présentation, le Lapidaire d’Alphonse X oriente l’interprétation de l’œuvre. Ces paratextes mettent en avant le rôle capital du savoir, représenté par la métaphore du trésor caché, qui a ici une valeur topique. Ils mettent également en exergue le rôle que s’attribue le roi dans la transmission de ce savoir, et placent cette nouvelle attribution royale au cœur de son projet politique.A través del prólogo y las miniaturas de presentación, el Lapidario de Alfonso X orienta la interpretación de la obra. Estos paratextos subrayan el papel capital del saber, representado por la metáfora del tesoro escondido, que tiene aquí un valor tópico. Ponen asimismo de manifiesto el papel que se atribuye el monarca en la transmisión de ese saber y colocan esta nueva atribución real en el centro de su proyecto político.

  9. Aventures radiophoniques du Nouveau Roman

    Directory of Open Access Journals (Sweden)

    Beatrice Nickel

    2015-12-01

    Full Text Available  Aventures radiophoniques du Nouveau Roman Colloque international organisé par l’Université Paul-Valéry Montpellier, en partenariat avec l’Université de Stuttgart 19-20 novembre 2015   Organisateurs :  Pierre-Marie Héron, Annie Pibarot, Université Paul-Valéry Montpellier, centre de recherche Rirra21. Françoise Joly, Beatrice Nickel, Universität Stuttgart 

  10. Du comptoir à la toge

    OpenAIRE

    Buti, Gilbert

    2007-01-01

    Descendant d’une famille de petits notables du Dauphiné, Antoine Anthoine (1749-1826) qui n’a pas pu accéder à la magistrature se destine à la marchandise. Après un apprentissage à Marseille au comptoir des Seimandy, il est nommé, par ces négociants, régisseur pour leur commandite de Constantinople. La mobilisation de sa proche parenté et les soutiens des pouvoirs politiques locaux, nationaux et internationaux lui permettent d’obtenir une mission destinée à ouvrir le commerce de la Russie mér...

  11. Les esclaves du commandant Quiquandon

    OpenAIRE

    Tymowski, Michal

    2003-01-01

    Le 10 janvier 1895, Albert Grodet, gouverneur général du Soudan français, adressa au ministère des Colonies une dépêche accusant le commandant Quiquandon, dirigeant de la région Nord-Est (Ségou), d'exploiter illégalement un domaine sur lequel travaillent 140 esclaves. À partir de ce cas particulier, l'auteur analyse les conflits qui existaient à l'époque entre l'administration civile et un groupe d'officiers soudanais. Il se demande si la possession d'esclaves par des officiers, prenant...

  12. Assessment of risks associated to ionizing radiations: lung cancers after domestic radon exposure and thyroid cancers after accidental exposure to radioactive iodines; Evaluation des risques associes aux rayonnements ionisants: cancers du poumon apres exposition domestique au radon et cancers de la thyroide apres exposition accidentelle aux iodes radioactifs

    Energy Technology Data Exchange (ETDEWEB)

    Catelinois, O

    2004-09-15

    The aim of this work is to develop a critical analysis of quantitative risk assessment in the field of ionizing radiation and to provide new estimates of attributable risks for particular situations of environmental exposure to ionizing radiation. This work is based on knowledge about dose-response relationships and ionizing radiation exposure of the general population. The work focuses on two different situations that both present an important interest for public health: lung cancer associated with domestic radon exposures (natural situation) and thyroid cancer associated with the Chernobyl accident fallout (accidental situation). The assessment of lung cancer risk associated with domestic radon exposure considers 10 dose-response relationships resulting from miner cohorts and case-control studies in the general population. A critical review of available data on smoking habits has been performed and allowed to consider the interactions between radon and tobacco. The exposure data come from measurements campaigns carried out since the beginning of the 1980 by the Institute for Radiation protection and Nuclear Safety and the Health General Directory in France. The French lung cancer mortality data are provided by the I.N.S.E.R.M.. Estimates of the number of attributable cancers are carried out for the whole country, stratified by 8 large regions (Z.E.A.T.) and by 96 departments for the year 1999 allowing to perform a sensibility analysis according to the geographical level of calculation. Uncertainties associated to risk coefficients and exposures have been quantified and it's impact on risk estimates is calculated. The estimated number of deaths attributable to domestic radon exposure ranges from 543 (90% uncertainty interval (U.I.): 75-1,097) to 3,108 (90% U.I.: 2,996-3,221). The corresponding risk fractions range from 2.2% (90% U.I.: 0.3%-4.4%) to 12.4% (90% U.I.: 11.9%-12.8%). The assessment of thyroid cancer risk in the most exposed area of France due to

  13. Collagen VI: A New Candidate Breast Cancer Marker Linked to Resistance to Platinum-Based Cancer Drugs

    Science.gov (United States)

    2012-09-01

    are expected to have more relevance to obesity-related cancers, such as colon, renal, pancreas, and post- menopausal breast cancer. My studies...correlates with the COL6A3 levels. A. PyMT mice were given TZD containing chow (supply approx. 20mg/kg/day TZD ) or normal- diet (ND) starting at 8-weeks of

  14. Volume da gota dos colírios lubrificantes: estudo farmacoeconômico

    Directory of Open Access Journals (Sweden)

    Alexandre Xavier da Costa

    2015-12-01

    Full Text Available RESUMO Objetivo: Determinar o volume médio das gotas produzidas pelos colírios lubrificantes em diferentes ângulos de inclinação e determinar o custo médio do tratamento. Métodos: Determinação do volume da gota de 3 frascos originais dos colírios lubrificantes Artelac®, Hylo Comod®, Lacrima® Plus, Systane® UL, Lacrifilm®, Hyabak®, Lacribell®, Ecofilm®, Mirugell®, Plenigell®, Fresh Tears®, Optive® e Endura® à inclinação de 90º e 45º. Determinou-se o número médio de gotas em cada frasco e foi feita avaliação farmacoeconômica dos colírios. Resultados: O volume das gotas variou de 32,2 a 64,0 µL a 45o e de 29,1 a 65,1 µL a 90o. A diferença entre as gotas em cada inclinação foi de 2 a 24% e o custo anual dos colírios de acordo com a inclinação variou de R$2,73 a R$130,73. A Duração Máxima de Tratamento (DMT foi de 29,3 a 51,4 dias na inclinação de 45o, e de 28,8 a 48,4 dias a 90º, sendo que a diferença na DMT foi de 0,5 até 8 dias a mais ou a menos, de acordo com a marca. Conclusão: Nenhum dos colírios estudados apresentou gotas ideais para o olho humano, levando a um desperdício do produto e maior custo para o fabricante e para o consumidor. Percebemos que existe uma variação significativa no volume da gota de acordo com a inclinação do frasco, e que uma variação maior do que 10% traria impactos financeiros para o paciente.

  15. Stickler syndrome caused by COL2A1 mutations: genotype-phenotype correlation in a series of 100 patients

    DEFF Research Database (Denmark)

    Hoornaert, Kristien P; Vereecke, Inge; Dewinter, Chantal;

    2010-01-01

    in COL2A1. In 188 probands with the clinical diagnosis of Stickler syndrome, the COL2A1 gene was analyzed by either a mutation scanning technique or bidirectional fluorescent DNA sequencing. The effect of splice site alterations was investigated by analyzing mRNA. Multiplex ligation......-dependent amplification analysis was used for the detection of intragenic deletions. We identified 77 different COL2A1 mutations in 100 affected individuals. Analysis of the splice site mutations showed unusual RNA isoforms, most of which contained a premature stop codon. Vitreous anomalies and retinal detachments were...... found more frequently in patients with a COL2A1 mutation compared with the mutation-negative group (P90% of the mutations were predicted to result in nonsense-mediated decay. On the basis of binary regression analysis, we developed a scoring system that may be useful when evaluating patients...

  16. Cancer

    Science.gov (United States)

    ... uses a surgical tool to remove the tumor.Mohs' surgery. Layers of cancer cells are removed one ... usually have not been approved by the U.S. Food and Drug Administration (FDA). The medicine may have ...

  17. Rejets et environnement du CERN

    CERN Document Server

    Vojtyla, P

    2007-01-01

    La radioactivité de l’environnement autour de l’Organisation Européenne pour la Recherche Nucléaire (CERN) et les doses de rayonnements qui en résultent pour la population avoisinante sont contrôlées par la Commission de Sécurité du CERN et de manière indépendante par les autorités compétentes des deux États Hôtes, l’Institut de Radioprotection et de Sûreté Nucléaire (IRSN) côté France et l’Office Fédéral de la Santé Publique (OFSP) côté Suisse (voire Chapitre 8.6). Dans ce rapport, les résultats de mesures concernent en particulier le territoire suisse. L’ensemble des mesures effectuées en 2006 prouve que le fonctionnement des installations du CERN était sans conséquence radiologique sur l’environnement et la population. Le contrôle des émissions montre que les rejets effectifs se situent, également en 2006, nettement en dessous des limites réglementaires. Ce constat est confirmé par le contrôle des immissions dans l’environnement. Le CERN a bien respecté en 2...

  18. Sociologie et sociolinguistique du franbreu

    Directory of Open Access Journals (Sweden)

    Ben-Rafael Eliezer

    2014-07-01

    Full Text Available Des enquêtes ont montré que plus de 20 % des adultes israéliens ont quelque connaissance du français. Parmi cette population, 250 000 à 300 000 personnes pouvaient être considérées comme francophones. Le français a affirmé une présence importante – pas toujours reconnue – dès la phase cruciale du développement de cette société. Une immigration de masse suivit la création de l’État (1948 dont la population a quasiment triplé en moins de deux décennies, avec l’arrivée de plus de 200 000 immigrants d’Afrique du Nord et des dizaines de milliers des Balkans et du Moyen-Orient. Beaucoup parmi ces immigrants étaient francophones – le français étant pour eux soit la première langue soit une deuxième qu’ils contrôlaient. C’est depuis lors que la présence du français est devenue démographiquement significative dans la société israélienne, se prolongeant de nos jours dans l’immigration en provenance de France qui se poursuit jusqu’à aujourd’hui. Comme nous l’avons montré ailleurs (Ben-Rafael et Ben-Rafael 2013, Israël fait l’expérience simultanée de plusieurs francophonies originales qui diffèrent par leurs sources aussi bien que leurs expressions (Ben-Rafael et Ben-Rafael 2013. Un premier modèle est le « français vernaculaire » (voir Boudras-Chapon 2008 amené par les immigrants de classes défavorisées arrivant d’Afrique du nord qui se sont regroupés en Israël. Leur français n’a offert qu’une faible résistance à l’hébreu qui s’est imposée dans le processus d’intégration. Un bilinguisme fonctionnant sur un mode additif au début qui s’est mué progressivement en modèle soustractif. Un second modèle concerne les immigrants de même origine ainsi que des immigrants francophones d’autres pays (pays balkaniques, Turquie ou Egypte formant une strate instruite de classe moyenne, pour qui le français était d’utilisation quotidienne aussi bien que de langue de

  19. The ColRS system of Xanthomonas oryzae pv. oryzae is required for virulence and growth in iron-limiting conditions.

    Science.gov (United States)

    Subramoni, Sujatha; Pandey, Alok; Vishnu Priya, M R; Patel, Hitendra Kumar; Sonti, Ramesh V

    2012-09-01

    Xanthomonas oryzae pv. oryzae, the causal agent of bacterial blight of rice, produces siderophores only under iron-limiting conditions. We screened 15 400 mTn5-induced mutants of X. oryzae pv. oryzae and isolated 27 mutants that produced siderophores even under iron-replete conditions. We found that the mTn5 insertions in 25 of these mutants were in or close to six genes. Mutants with insertions in five of these genes [colS, XOO1806 (a conserved hypothetical protein), acnB, prpR and prpB] exhibited a deficiency for growth on iron-limiting medium and a decrease in virulence. Insertions in a sixth gene, XOO0007 (a conserved hypothetical protein), were found to affect the ability to grow on iron-limiting medium, but did not affect the virulence. Targeted gene disruptants for colR (encoding the predicted cognate regulatory protein for ColS) also exhibited a deficiency for growth on iron-limiting medium and a decrease in virulence. colR and colS mutants were defective in the elicitation of hypersensitive response symptoms on the nonhost tomato. In addition, colR and colS mutants induced a rice basal defence response, suggesting that they are compromised in the suppression of host innate immunity. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis demonstrated that a functional ColRS system is required for the optimal expression of several genes encoding components of the type 3 secretion system (T3SS) of X. oryzae pv. oryzae. Our results demonstrate the role of several novel genes, including colR/colS, in the promotion of growth on iron-limiting medium and the virulence of X. oryzae pv. oryzae.

  20. L’interface Habitat / Habitant : quand le lieu de vie est porteur de maladie - Le cas du radon en PACA

    Directory of Open Access Journals (Sweden)

    Cissé E.

    2012-10-01

    Full Text Available Les préoccupations sont croissantes pour étudier les liens entre des conditions environnementales spécifiques et la fréquence de certaines pathologies. Nous proposons d’analyser ici le lien présupposé entre une exposition au radon et le cancer du poumon. Le radon, est un gaz radioactif d’origine naturelle (issu de la désintégration naturelle de l’uranium présent dans la croûte terrestre et plus particulièrement dans certaines formations géologiques. A partir du sol et de l’eau, le radon se diffuse dans l’air (exhalation et se trouve, par effet de confinement, à des concentrations plus élevées à l’intérieur des bâtiments qu’à l’extérieur. Les descendants solides du radon sont alors inhalés avec l’air respiré et se déposent dans le poumon. Il est établi par diverses études scientifiques que le radon a un impact non négligeable sur certains cancers, notamment du poumon (augmentation de 16 % du risque de cancer du poumon par tranche de 100 Bq/m3, et des cancers digestifs via la consommation d’eau qui s’est chargée en radon au contact du socle. Le nombre annuel de décès par cancer du poumon attribuable à l’exposition domestique au radon en France métropolitaine varie entre 1 200 à 3 575 en fonction des relations exposition-réponse utilisées. Plusieurs régions en France se caractérisent par une problématique radon reconnue et étudiée depuis longtemps (Bretagne, Massif central, Corse. Or, si le radon ne recouvre pas l’ensemble de la région PACA, certaines zones géographiques sont particulièrement concernées et méritent d’être étudiées : le Massif des Maures dans le Var, du Mercantour dans les Alpes-Maritimes et du Briançonnais dans les Hautes-Alpes. La région PACA est une des régions de France et d’Europe les plus touchées par la pollution atmosphérique principalement due à l’augmentation du trafic routier et des contributions de régions industrialisées telles que

  1. Le cas du Haut-Adige ou Tyrol du Sud

    Directory of Open Access Journals (Sweden)

    Licia Bagini Scantamburlo

    2007-06-01

    Full Text Available La situation de cette région italienne est vraiment emblématique des rapports entre « les territoires et l’identité » à cause de la présence de différentes communautés linguistiques autochtones, dont les plus importantes sont les minorités germanophone et latine. S’il est vrai que cette situation existe également dans d’autres régions italiennes, il est incontestable que dans le Haut-Adige/Tyrol du Sud elle présente des caractéristiques très spécifiques et uniques pour l’Italie, car les italo...

  2. De Sumer à Seattle, le voyage du bout du monde

    Directory of Open Access Journals (Sweden)

    Olivier Walther

    2008-12-01

    Full Text Available « Les affaires qui se nouent par-dessus les frontières qui séparent les peuples ne font pas que ces frontières n'existent pas » notait Émile Durkheim en 1895 (1895 : 113. Un peu plus d’un siècle plus tard, l’ouvrage de William J. Bernstein intitulé A Splendid Exchange et sous-titré de manière inspirée How Trade Shaped the World vient apporter une confirmation étayée au sociologue français. De Sumer où fut introduit le commerce du cuivre 3000 ans avant Jésus-Christ, à Seattle où se tint la tr...

  3. COMMUNICATION DU CREDIT AGRICOLE - French version only

    CERN Multimedia

    2003-01-01

    La Direction du Crédit Agricole informe son aimable clientèle du CERN des jours et heures d'ouverture de l'agence du site de Prévessin à compter du mardi 14 janvier 2003 : 1. Horaires pour les opérations bancaires courantes 7 jours sur 7 et 24h/24 avec l'espace libre service bancaire. 2. Horaires conseil du mardi au vendredi - Mardi, de 9h. à 12h. et de 14h.15 à 16h.30. - Mercredi, jeudi et vendredi, de 9h. à 12h. et de 13h.30 à 16h.30. Deux collaboratrices au lieu d'une seront désormais présentes toute la journée du mardi au vendredi pour vous accueillir, vous informer et vous conseiller en crédits et placements (réception conseil sur rendez-vous). Autre nouveauté : les mêmes conseillers seront aussi à votre disposition le samedi, sur notre agence de Gex, de 8h.15 à 13h.05, notamment pour les études de financements habitat. La Direction et toute l'équipe de l'agence du Crédit Agricole vous souhaitent une excellent année 2003.

  4. Approche de prise en charge du trouble du spectre de l’autisme

    Science.gov (United States)

    Lee, Patrick F.; Thomas, Roger E.; Lee, Patricia A.

    2015-01-01

    Résumé Objectif Se pencher sur les critères diagnostiques du trouble du spectre de l’autisme (TSA) comme les définit le Manuel diagnostique et statistique des troubles mentaux, cinquième édition (DSM-V), et concevoir une approche de prise en charge du TSA à l’aide du cadre CanMEDS–Médecine familiale (CanMEDS-MF). Sources d’information Le DSM-V, publié par l’American Psychiatric Association en mai 2013, énonce de nouveaux critères diagnostiques du TSA. Le cadre CanMEDS-MF du Collège des médecins de famille du Canada fournit un plan d’orientation pour la prise en charge complexe du TSA. Nous avons utilisé des données recueillies par le Centers for Disease Control and Prevention afin de déterminer la prévalence du TSA, ainsi que la revue systématique et méta-analyse détaillée effectuée par le National Institute for Health and Care Excellence du R.-U. pour ses lignes directrices sur le TSA dans le but d’évaluer les données probantes issues de plus de 100 interventions. Message principal Selon les données du Centers for Disease Control and Prevention, la prévalence du TSA se chiffrait à 1 sur 88 en 2008 aux États-Unis. La classification du TSA dans la quatrième édition du DSM incluait l’autisme, le syndrome d’Asperger, le trouble envahissant du développement et le trouble désintégratif de l’enfance. La dernière révision du DSM-V réunit tous ces troubles sous la mention TSA, avec différents niveaux de sévérité. La prise en charge du TSA est complexe; elle exige les efforts d’une équipe multidisciplinaire ainsi que des soins continus. Les rôles CanMEDS-MF fournissent un cadre de prise en charge. Conclusion Les médecins de famille sont au cœur de l’équipe de soins multidisciplinaire pour le TSA, et le cadre CanMEDS-MF tient lieu de plan détaillé pour guider la prise en charge d’un enfant atteint de TSA et aider la famille de cet enfant.

  5. Nouvelles du Centre Aéré de l’Association du Personnel du CERN

    CERN Multimedia

    Jardin d'enfants

    2015-01-01

    Cet été 2015, durant quatre semaines d’été, le Centre Aéré a accueilli plus de 40 enfants âgés de 4 à 6 ans. Devant le succès rencontré, et à la demande des parents, il a été décidé d’en doubler la capacité maximale. A l'été 2016, du 4 au 29 juillet, la structure pourra accueillir les enfants de 4 ans révolus et de moins de 7 ans (nés après le 31/07/2009 mais avant  01/07/2012). Les inscriptions se feront à la semaine durant le mois d'Avril 2016. Les programmes sont en cours d'élaboration cependant nous pouvons déjà vous communiquer le thème conducteur du centre qui sera : à la découverte d'un continent différent chaque ...

  6. Sylvain Fagot, Le cirque. Entre culture du corps et culture du risque

    OpenAIRE

    Crochet-Giacometti, Nicolas

    2011-01-01

    Cet ouvrage écrit par Sylvain Fagot, chercheur en sociologie associé à l'université du Québec, et auteur d'une thèse soutenue en 2003 intitulée « Ethno-sociologie du cirque. Arts de la piste / Arts du corps / Arts du risque », s'intéresse à un monde peu connu : celui du cirque. L'objectif y est de montrer comment chaque forme du cirque, le cirque dit « classique » et le cirque dit « nouveau », ont des stratégies sociales et économiques différentes mais surtout un rapport au corps et au risque...

  7. Study and progress of collagen Ⅳ gene COL4A1 mutations%Ⅳ型胶原基因COL4A1突变的研究进展

    Institute of Scientific and Technical Information of China (English)

    安丽梅; 夏欣一

    2011-01-01

    Ⅳ型胶原蛋白基因α1 (COL4A1)编码Ⅳ型胶原的α1链,是各种组织基底膜的主要结构成分.基底膜由胶原、层粘连蛋白、蛋白多糖和巢蛋白组成,在所有胶原成分中,最重要的是Ⅳ型胶原.COL4A1基因突变能引起多种疾病,在一定的环境压力下会引发HANAC综合征、肾病、孔洞脑、白内障等疾病.本文主要从COL4A1的结构、组成及在基底膜中的作用等方面,对COI4A1基因突变引发的人类疾病及其发病机制进行综述.%α1 type IV collagen (COL4A1) encodes the α1 chain of type Ⅳ collagen. Collagen type Ⅳ is the most abundant structural BM component in a variety of tissues. In general, Basement membranes ( BMs) are composed of collagens, laminins, perle-can, and nidogens. Collagen IV is important to BM. Mutations in COL4A1 gene have been linked to a spectrum of diverse diseases caused by abnormalities of these molecules in the kidney, brain and eyes. In this review, we will cover, after a short overview of the molecular basis of the COL4A1 and the main BM components, the current knowledge of diseases caused by COL4A1 mutations and summarize the possible mechanisms of mutations and human diseases.

  8. Chapitre VI. Prudence du financement

    OpenAIRE

    Jambard, Pierre

    2015-01-01

    L’histoire financière de la société permet de comprendre bien des traits de son histoire. Comme toutes les entreprises du bâtiment et des travaux publics, la Société Auxiliaire s’est trouvée devant la nécessité de disposer d’importants capitaux circulants. Grâce à une gestion financière efficace, elle est, en général, parvenue à dégager de son activité les fonds nécessaires, bien que l’aisance de la trésorerie quotidienne n’ait été acquise qu’avec la reconversion. Les années 1927-1946 sont ce...

  9. L’abolition du vieillissement

    Directory of Open Access Journals (Sweden)

    Josef Reindl

    2012-02-01

    Full Text Available I « L’humanité vieillit à un rythme inimaginable. (... »« L’humanité vieillit à un rythme inimaginable. Nous devons résoudre le problème de notre propre vieillissement pour résoudre celui du monde entier. » M. Schirrmacher, l’un des intellectuels médiatiques les plus connus de l’Allemagne, joue comme toujours de façon magistrale la partition de l’effroi. Il ne se contente nullement de s’arranger avec son propre vieillissement, mais il veut sauver le monde à travers « une révolution militante...

  10. DU COTÉ DE CHEZ SOI

    OpenAIRE

    Marc Smeets

    2013-01-01

    Le voyage en Hollande, au XIXe siècle, s'inscrit dans une longue tradition où résonnent les notions de liberté, tolérance et commerce, mais il acquiert aussi une spécificité étant donné l’importance que prend la figure du « chez soi » : les Pays-Bas, terre d'agrément où le voyageur français se sent à l'aise et où il aimerait, si possible, vivre. Proust, de ce point de vue-là, ne fait rien de neuf quand il rêve dans la Recherche d'une « vie domestique » en terre batave. Les Pays-Bas, pour le v...

  11. Sulphite metabolism; Metabolisme du sulfite

    Energy Technology Data Exchange (ETDEWEB)

    Fromageot, P.; Chapeville, F. [Commissariat a l' Energie Atomique, Saclay (France).Centre d' Etudes Nucleaires

    1960-07-01

    Although the formation of sulphite by micro-organisms was observed as early as 1914 by Neuberg, it was only in 1932 that Nightingale showed it to be present inside cells, in the case of tomato leaf stalks, its formation being due to the reaction: SO{sub 3}H{sup -} + (S{sub 2}O{sub 3}){sub 2}{sup --} {yields} S{sub 2}O{sub 3}{sup --} + O{sub 3}S{sub 2}O{sub 3}{sup --}. The presence of the thiosulphate formed was shown by the high refractive index of its barium salt. Today we have a certain amount of data concerning the formation and use of sulphite in the living cell, even through the knowledge is still incomplete. In this article we will describe and discuss the facts which are known, with particular reference to the oxidation of sulphite. (author) [French] Si la formation de sulfite par des micro-organismes a ete observee des 1914 par Neuberg, sa presence a l'interieur des cellules a ete demontree pour la premiere fois par Nightingale en 1932, dans des tiges et des feuilles de tomates, par la reaction: SO{sub 3}H{sup -} + (S{sub 2}O{sub 3}){sub 2}{sup --} {yields} S{sub 2}O{sub 3}{sup --} + O{sub 3}S{sub 2}O{sub 3}{sup --}. Le thiosulfate forme etait mis en evidence par l'indice de refraction eleve de son sel de baryum. Aujourd'hui on possede un certain nombre de donnees, cependant encore tres incompletes, sur la formation du sulfite et son utilisation par la cellule vivante. Dans cet expose nous decrirons et discuterons quelques-unes de ces acquisitions, tout particulierement celles relatives a l'oxydation du sulfite. (auteur)

  12. La place de l'imagerie par résonance magnétique dans le carcinome lobulaire du sein

    Science.gov (United States)

    Bouzoubaa, Wail; Laadioui, Meryem; Alaoui, Fatime Zahra Fdili; Jayi, Sofia; Bouguern, Hakima; Chaara, Hikmat; Melhouf, Moulay Abdelilah

    2014-01-01

    Le carcinome lobulaire reste une entité histologique peu fréquente du cancer du sein, toute fois la place qu'occupe le cancer du sein actuellement dans la cancérologie féminine, justifie la connaissance des particularités de ce type de cancer mammaire. Le diagnostic paraclinique est basée sur le couple écho-mammographie a la recherche de multifocalité, multicentricité ou bilatéralité, d'où l'intérêt de l'IRM qui est la technique la plus sensible pour la mise en évidence de ces lésions et qui est devenue un examen de pratique courante dans le carcinome lobulaire du sein. Par le présent travail, et sous la lumière de la revue de la littérature, nous allons essayer de dégager les aspects épidémiologiques, cliniques, et paracliniques, du carcinome lobulaire du sein, et insister sur les indications et l'intérêt de l'IRM dans la prise en charge de ce type histologique. PMID:25368710

  13. A novel COL11A1 mutation affecting splicing in a patient with Stickler syndrome.

    Science.gov (United States)

    Kohmoto, Tomohiro; Naruto, Takuya; Kobayashi, Haruka; Watanabe, Miki; Okamoto, Nana; Masuda, Kiyoshi; Imoto, Issei; Okamoto, Nobuhiko

    2015-01-01

    Stickler syndrome is a clinically and genetically heterogeneous collagenopathy characterized by ocular, auditory, skeletal and orofacial abnormalities, commonly occurring as an autosomal dominant trait. We conducted target resequencing to analyze candidate genes associated with known clinical phenotypes from a 4-year-old girl with Stickler syndrome. We detected a novel heterozygous intronic mutation (NM_001854.3:c.3168+5G>A) in COL11A1 that may impair splicing, which was suggested by in silico prediction and a minigene assay.

  14. Colágeno asimilable. Fuente de prevención de enfermedades osteoarticuladas

    OpenAIRE

    Parra Parra, Yolanda; Quesada Martínez, María Inmaculada; Pérez Collado, Elisa María

    2015-01-01

    Cartel presentado en la Segunda Conferencia Internacional de Comunicación en Salud, celebrada el 23 de octubre de 2015 en la Universidad Carlos III de Madrid Introducción: El colágeno es la proteína más abundante de nuestro cuerpo humano y uno de sus componentes esencial de articulaciones, cartílago, ligamento, tendones, huesos, piel. Su especial estructura lo hacen único, presenta una estructura fibrosa, que aporta gran resistencia y flexibilidad a los tejidos de los que forma parte. Cu...

  15. Col-OSSOS: A new ugrJ taxonomy for trans-Neptunian objects

    Science.gov (United States)

    Fraser, Wesley Cristopher; Bannister, Michele T.; Marsset, Michael; Pike, Rosemary E.; Schwamb, Megan E.; Kavelaars, J. J.; Benecchi, Susan D.; Delsanti, Audrey; Lehner, Matt J.; Wang, Shiang-Yu; Thirouin, Audrey; Guilbert-Lepoutre, Aurelie; Peixinho, Nuno; Vernazza, Pierre

    2016-10-01

    The surfaces of trans-Neptunian objects (TNOs) are poorly understood. Very little has been discerned about the compositions of most small TNOs. In recent years however, some concrete knowledge about the surface colour distribution of TNOs has come to light. It is now generally accepted that small TNOs fall into at least three classes of object based on their surface colours and albedo. Despite nearly two decades of gathering TNO surface information however, a taxonomy has still not been agreed upon. From Col-OSSOS u, g, r, and J photometry, we find significantly different clustering of (u-g) colour in the optically red, dynamically cold TNOs as compared to similarly optically coloured dynamically excited TNOs. One of the goals of the Colours of the Outer Solar System Origins Survey is the development of a robust TNO taxonomy. This 4 year program which started in 2014B is simultaneously using the Gemini-North and Canada-France-Hawaii telescopes to gather near simultaneous u, g, r, and J spectral photometry of all targets in the Outer Solar System Origins Survey (OSSOS) brighter than r'=23.6 (120 expected). The focus of Col-OSSOS is completeness and consistency, with the same SNR=25 being reached in all bands, for all targets brighter than our depth limit. Col-OSSOS will provide the first brightness-complete, compositional-dynamical map of the Outer Solar System, from which key hypotheses about the Solar System's cosmogony can be tested. After an overview of the survey's design and techniques, we will present the observed colours from the first complete block. Even with just ~30 targets, the precise photometry afforded by Col-OSSOS has already revealed the existence of 3 separate TNO taxons or classes, which become obvious when their (u-g), (g-r), and (r-J) colours are considered together. In particular, the so-called cold classical TNOs, which stand out because of their dynamically quiescent orbits, while possessing similar (g-r) and (r-J) colours as other red TNOs

  16. Influencia de pesticidas en las poblaciones de Colémbolos

    OpenAIRE

    Durán Vicente, Alberto M.

    2013-01-01

    El efecto de los insecticidas en las poblaciones de Colémbolos fue investigado en una finca de frutales en la localidad de Nitra (Eslovaquia). El estudio se hizo en dos parcelas, una en la que se aplicaron insecticidas de manera esporádica y controlada, y la otra, en la cual no se habian utilizado pesticidas desde hacía años. Los tratamientos fueron establecidos para investigar la influencia de los insectos presentes sobre y bajo la capa superficial del suelo, en la sucesión vegetal. Lo...

  17. Inauguration du C/R Forum (Belgique

    Directory of Open Access Journals (Sweden)

    2009-04-01

    Full Text Available Le 16 février dernier, la Vlaamse beroepsvereniging Conservatie en Restauratie Forum (C/R Forum a été constituée à l'initiative de 18 personnes du milieu de la conservation/restauration et du secteur de la protection du patrimoine. Etant donné que la conservation et la gestion du patrimoine culturel mobilier et immobilier de même que la formation et la recherche relèvent des communautés et des régions, la nécessité est apparue depuis un certain temps de créer une organisation Flamande au sei...

  18. Daphne du Maurier' unustatud jutud / Udo Uibo

    Index Scriptorium Estoniae

    Uibo, Udo, 1956-

    2011-01-01

    Ajalehe "The Independent" teatel kavatseb kirjastus "Virago" maikuus avaldada Daphne Du Maurier lühiproosa kogumiku "Nukk" (The Doll), mille on koostanud tema loomingu suur austaja Ann Willmore ja mis sisaldab 13 unustatud ja seni avaldamata novelli

  19. Flaubert et Du Camp : quelques remarques

    Directory of Open Access Journals (Sweden)

    Michel Brix

    2016-12-01

    Full Text Available Les spécialistes de Flaubert ont fait de nombreux reproches à Maxime Du Camp et ne prêtent plus guère d’attention à ce qu’il a écrit. C’est une situation très regrettable, qui prive les lecteurs d’informations nombreuses, susceptibles d’éclairer les enjeux mais aussi les impasses et les contradictions du projet esthétique de Flaubert. Quelques cas significatifs sont évoqués dans le présent article, qui se penche plus particulièrement sur la ressemblance entre un passage du livre de Du Camp Le Nil (1854 et les lignes de L’Éducation sentimentale (1869 qui rapportent l’“apparition” de Mme Arnoux.

  20. La fin du risque zéro : du homegrown jihadism au terrorisme du loup solitaire

    Directory of Open Access Journals (Sweden)

    Rémi Baudouï

    2015-09-01

    Full Text Available Le risque zéro représente un moment historique particulier dans la conceptualisation de la socio-politique du risque. Son application repose au début des années 1980 sur l’hypothèse de la possibilité de progresser dans le domaine de l’anticipation et de la gestion sécuritaire des crises potentielles au point de pouvoir prémunir les populations contre toute forme de menace. Il fut employé dans les domaines de la protection civile et de la sécurité militaire. Comme élément de gouvernance politique, il réfutait l’idée selon laquelle le risque existe en préalable comme catégorie d’acceptabilité collective de la menace.

  1. Aux frontières du politique et du religieux

    Directory of Open Access Journals (Sweden)

    Frédéric Sylvanise

    2006-05-01

    Full Text Available The Autobiography of Miss Jane Pittman met en scène de manière spectaculaire deux figures de leadership noir-américain au XIXe siècle (Ned et au XXe siècle (Jimmy. Ned, fils adoptif de Jane Pittman, se rebaptise Professor Douglass en hommage au leader abolitionniste Frederick Douglass. S’il est fondamentalement laïc (il est instituteur, le texte lui confère néanmoins une dimension religieuse, notamment dans le chapitre intitulé « The Sermon at the River » , qui renvoie à la fois à Moïse (le fleuve et à Jésus (le sermon sur la montagne. Abattu par un tueur à gages, Ned est un martyr dont l’histoire était déjà écrite. Le personnage de Jimmy, attendu par toute une communauté comme le messie (alors que lui aussi est un laïc et un homme qui perd la foi, s’inscrit dans la même histoire. Lettré, éloquent et impliqué dans le mouvement pour les droits civils, il est assassiné comme son prédécesseur. Dans les deux cas, le texte lie donc le politique et le biblique comme s’il ne faisait qu’un : être un leader politique, c’est être un preacher et un messie qui guide le peuple. Il est possible de lire l’évocation de ces deux hommes à la lumière d’une certaine actualité. Ned et Jimmy connaissent en effet un sort proche de celui de Malcolm X et de Martin Luther King, récemment assassinés au moment de l’écriture de The Autobiography of Miss Jane Pittman. C’est l’histoire de la répression du leadership noir, au travers du portrait de deux de ses avatars, qui peut donc se lire en creux ici.

  2. O Crisol: periódico das alunas do Colégio Americano (Porto Alegre/RS, 1945-1964 - O Crisol: the jornal by students of the Colégio Americano (Porto Alegre/RS, 1945-1964

    Directory of Open Access Journals (Sweden)

    Dóris Bittencourt Almeida, Brasil

    2013-04-01

    PERIÓDICO DE LAS ALUMNAS DEL COLÉGIO AMERICANO (PORTO ALEGRE/RS, 1945-1964  ResumenEste artículo investiga el periódico O Crisol, producido por las alumnas del Colegio Americano, una escuela de Porto Alegre/RS entre los años 1945 y 1964. Lo periódico dice mucho a la Historia de la Educación, como lo evidenciael conocimiento y las prácticas que circulaban en la comunidad del Colegio, revelando un poco de las representaciones de la cultura juvenil de la época. Acá interesan las significaciones de los discursos difundidos por el periódico, en los procesos de subjetivación causados por los textos y sus posibles influencias en la construcción de las identidades de las jóvenes lectoras y escritoras del periódico. La pesquisa se identifica con los presupuestos teóricos de la Historia Cultural y si inscribe en el campo de la lectura y escrita de los recuerdos de la juventud, tomando como referencia los conceptos de alfabetización como producción discursiva de un determinado tiempo y lugar.Palabras-clave: recuerdos juveniles, escritos escolares, prácticas de escritura.  O CRISOL: PERIODIQUE DES ÉTUDIANTS DU COLÉGIO AMERICANO(PORTO ALEGRE/RS, 1945-1964RésumeCet étude examine le periodique O Crisol, realisé par les étudiants du Colégio Americano, une école de Porto Alegre/RS,entre les années 1945 et 1964.Le journaux disent beaucoup à l’Histoire de l’Education,comme en témoignant les connaissances et les pratiques qui circulaient dans la communauté,en revelant un peu de representations de la culture de la jeunesse de l’époque.Voici les sens de les intérêts de la difusion des discours diffusés par la revue, dans les processus de subjectivation causés par les textes et leurs influences possibles sur la construction des identités de les jeunes lecttrices et des écrivains du jornal.La recherche identifie les pressupposés théoriques de l’Histoire Culturelle et inscrit dans le domaine de les pratiques de lecture et de l’écriture et

  3. Auto-imunidade e colágeno V Autoimmunity and collagen V

    Directory of Open Access Journals (Sweden)

    Cristiane Carla de Oliveira

    2006-06-01

    Full Text Available As proteínas da matriz extracelular (MEC e seus componentes estão sendo amplamente estudados na literatura médica, assim como sua relação com o remodelamento tecidual presente nas doenças reumáticas. Neste artigo, mostramos a importância do estudo do colágeno do tipo V no entendimento da etiologia da esclerodermia, no que se refere ao desencadeamento da auto-imunidade nesta enfermidade. Estudos em nosso laboratório demonstram que a sensibilização com colágeno do tipo V em coelhos pode resultar em um modelo animal de esclerodermia. Diante destes fatos, sugerimos que pesquisas neste campo podem ser de grande valia no desenvolvimento de novas condutas terapêuticas.The extracellular matrix (ECM proteins and their components have been widely studied in medical literature, as well its relationship with the tecidual remodeling present in the rheumatic disease. In this paper we show the importance of understanding the role of type V collagen as an important trigger of rheumatic autoimmune diseases. Studies in our laboratory demonstrate that type V collagen sensibilization in rabbits, could result in an animal scleroderma model. In this way we suggested that researches in this field can be worthy in development of new therapeutic procedures.

  4. Artificial Autopolyploidization Modifies the Tricarboxylic Acid Cycle and GABA Shunt in Arabidopsis thaliana Col-0.

    Science.gov (United States)

    Vergara, Fredd; Kikuchi, Jun; Breuer, Christian

    2016-05-23

    Autopolyploidy is a process whereby the chromosome set is multiplied and it is a common phenomenon in angiosperms. Autopolyploidy is thought to be an important evolutionary force that has led to the formation of new plant species. Despite its relevance, the consequences of autopolyploidy in plant metabolism are poorly understood. This study compares the metabolic profiles of natural diploids and artificial autotetraploids of Arabidopsis thaliana Col-0. Different physiological parameters are compared between diploids and autotetraploids using nuclear magnetic resonance (NMR), elemental analysis (carbon:nitrogen balance) and quantitative real-time PCR (qRT-PCR). The main difference between diploid and autotetraploid A. thaliana Col-0 is observed in the concentration of metabolites related to the tricarboxylic acid cycle (TCA) and γ-amino butyric acid (GABA) shunt, as shown by multivariate statistical analysis of NMR spectra. qRT-PCR shows that genes related to the TCA and GABA shunt are also differentially expressed between diploids and autotetraploids following similar trends as their corresponding metabolites. Solid evidence is presented to demonstrate that autopolyploidy influences core plant metabolic processes.

  5. Artificial Autopolyploidization Modifies the Tricarboxylic Acid Cycle and GABA Shunt in Arabidopsis thaliana Col-0

    Science.gov (United States)

    Vergara, Fredd; Kikuchi, Jun; Breuer, Christian

    2016-05-01

    Autopolyploidy is a process whereby the chromosome set is multiplied and it is a common phenomenon in angiosperms. Autopolyploidy is thought to be an important evolutionary force that has led to the formation of new plant species. Despite its relevance, the consequences of autopolyploidy in plant metabolism are poorly understood. This study compares the metabolic profiles of natural diploids and artificial autotetraploids of Arabidopsis thaliana Col-0. Different physiological parameters are compared between diploids and autotetraploids using nuclear magnetic resonance (NMR), elemental analysis (carbon:nitrogen balance) and quantitative real-time PCR (qRT-PCR). The main difference between diploid and autotetraploid A. thaliana Col-0 is observed in the concentration of metabolites related to the tricarboxylic acid cycle (TCA) and γ-amino butyric acid (GABA) shunt, as shown by multivariate statistical analysis of NMR spectra. qRT-PCR shows that genes related to the TCA and GABA shunt are also differentially expressed between diploids and autotetraploids following similar trends as their corresponding metabolites. Solid evidence is presented to demonstrate that autopolyploidy influences core plant metabolic processes.

  6. Loss of Col3a1, the gene for Ehlers-Danlos syndrome type IV, results in neocortical dyslamination.

    Directory of Open Access Journals (Sweden)

    Sung-Jin Jeong

    Full Text Available It has recently been discovered that Collagen III, the encoded protein of the type IV Ehlers-Danlos Syndrome (EDS gene, is one of the major constituents of the pial basement membrane (BM and serves as the ligand for GPR56. Mutations in GPR56 cause a severe human brain malformation called bilateral frontoparietal polymicrogyria, in which neurons transmigrate through the BM causing severe mental retardation and frequent seizures. To further characterize the brain phenotype of Col3a1 knockout mice, we performed a detailed histological analysis. We observed a cobblestone-like cortical malformation, with BM breakdown and marginal zone heterotopias in Col3a1⁻/⁻ mouse brains. Surprisingly, the pial BM appeared intact at early stages of development but starting as early as embryonic day (E 11.5, prominent BM defects were observed and accompanied by neuronal overmigration. Although collagen III is expressed in meningeal fibroblasts (MFs, Col3a1⁻/⁻ MFs present no obvious defects. Furthermore, the expression and posttranslational modification of α-dystroglycan was undisturbed in Col3a1⁻/⁻ mice. Based on the previous finding that mutations in COL3A1 cause type IV EDS, our study indicates a possible common pathological pathway linking connective tissue diseases and brain malformations.

  7. COL-3, a chemically modified tetracycline, inhibits lipopolysaccharide-induced microglia activation and cytokine expression in the brain.

    Science.gov (United States)

    Edan, Rawan Abdulhameed; Luqmani, Yunus A; Masocha, Willias

    2013-01-01

    Microglia activation results in release of proinflammatory molecules including cytokines, which contribute to neuronal damage in the central nervous system (CNS) if not controlled. Tetracycline antibiotics such as minocycline inhibit microglial activation and cytokine expression during CNS inflammation. In the present study we found that administration of chemically modified tetracycline-3 (COL-3), inhibits lipopolysaccharide (LPS)-induced microglial and p38 MAPK activation, as well as the increase in TNF-α, but not IL-1β expression, in the brains of BALB/c mice. COL-3 has been described to have no antibacterial activity. We observed that COL-3 had no activity against a Gram-negative bacteria, Escherichia coli; however surprisingly, COL-3 had antibacterial activity against a Gram-positive bacteria Staphylococcus aureus, with a minimum inhibitory concentration of 1 mg/ml. Our data show that COL-3 has some antibacterial activity against S. aureus, inhibits LPS-induced neuroinflammation, and displays potential as a therapeutic agent for treatment of conditions involving CNS inflammation.

  8. COL-3, a chemically modified tetracycline, inhibits lipopolysaccharide-induced microglia activation and cytokine expression in the brain.

    Directory of Open Access Journals (Sweden)

    Rawan Abdulhameed Edan

    Full Text Available Microglia activation results in release of proinflammatory molecules including cytokines, which contribute to neuronal damage in the central nervous system (CNS if not controlled. Tetracycline antibiotics such as minocycline inhibit microglial activation and cytokine expression during CNS inflammation. In the present study we found that administration of chemically modified tetracycline-3 (COL-3, inhibits lipopolysaccharide (LPS-induced microglial and p38 MAPK activation, as well as the increase in TNF-α, but not IL-1β expression, in the brains of BALB/c mice. COL-3 has been described to have no antibacterial activity. We observed that COL-3 had no activity against a Gram-negative bacteria, Escherichia coli; however surprisingly, COL-3 had antibacterial activity against a Gram-positive bacteria Staphylococcus aureus, with a minimum inhibitory concentration of 1 mg/ml. Our data show that COL-3 has some antibacterial activity against S. aureus, inhibits LPS-induced neuroinflammation, and displays potential as a therapeutic agent for treatment of conditions involving CNS inflammation.

  9. Modeling near-surface firn temperature in a cold accumulation zone (Col du Dôme, French Alps): from a physical to a semi-parameterized approach

    OpenAIRE

    2014-01-01

    Analysis of the thermal regime of glaciers is crucial for glacier hazard assessment, especially in the context of a changing climate. In particular, the transient thermal regime of cold accumulation zones needs to be modeled. A modeling approach has therefore been developed to determine this thermal regime using only near-surface boundary conditions coming from meteorological observations. In the first step, a surface energy balance (SEB) model accounting for water percolation and radiation p...

  10. Description de trois espèces nouvelles du genre Oïdes, Weber (Coléoptères Phytophages)

    NARCIS (Netherlands)

    Duvivier, Ant.

    1884-01-01

    Convexe, ovalaire. — Antennes, thorax, cuisses (sauf l’extrémité des intermédiaires et des postérieures), et les quatre derniers segments de Vabdomen testacés; tête, écusson , poitrine, extrémité des cuisses intermédiaires et postérieures, tibias et tarses noirs; élytres brillantes, ponctuées, d’un

  11. CANCER

    Directory of Open Access Journals (Sweden)

    N. Kavoussi

    1973-09-01

    Full Text Available There are many carcinogenetic elements in industry and it is for this reason that study and research concerning the effect of these materials is carried out on a national and international level. The establishment and growth of cancer are affected by different factors in two main areas:-1 The nature of the human or animal including sex, age, point and method of entry, fat metabolism, place of agglomeration of carcinogenetic material, amount of material absorbed by the body and the immunity of the body.2 The different nature of the carcinogenetic material e.g. physical, chemical quality, degree of solvency in fat and purity of impurity of the element. As the development of cancer is dependent upon so many factors, it is extremely difficult to determine whether a causative element is principle or contributory. Some materials are not carcinogenetic when they are pure but become so when they combine with other elements. All of this creates an industrial health problem in that it is almost impossible to plan an adequate prevention and safety program. The body through its system of immunity protects itself against small amounts of carcinogens but when this amount increases and reaches a certain level the body is not longer able to defend itself. ILO advises an effective protection campaign against cancer based on the Well –equipped laboratories, Well-educated personnel, the establishment of industrial hygiene within factories, the regular control of safety systems, and the implementation of industrial health principles and research programs.

  12. La place de l'imagerie par résonance magnétique dans le carcinome lobulaire du sein

    OpenAIRE

    Bouzoubaa, Wail; Laadioui, Meryem; Alaoui, Fatime Zahra Fdili; Jayi, Sofia; Bouguern, Hakima; Chaara, Hikmat; Melhouf, Moulay Abdelilah

    2014-01-01

    Le carcinome lobulaire reste une entité histologique peu fréquente du cancer du sein, toute fois la place qu'occupe le cancer du sein actuellement dans la cancérologie féminine, justifie la connaissance des particularités de ce type de cancer mammaire. Le diagnostic paraclinique est basée sur le couple écho-mammographie a la recherche de multifocalité, multicentricité ou bilatéralité, d'où l'intérêt de l'IRM qui est la technique la plus sensible pour la mise en évidence de ces lésions et qui ...

  13. Comportement du carbone aux joints de grains du molybdène

    OpenAIRE

    1994-01-01

    Pas de résumé en anglais; La mise en évidence et l'interprétation du rôle bénéfique du carbone et du rôle néfaste de l'oxygène sur la cohésion intergranulaire du molybdène en l'absence de précipitation visible au MEB constituaient le but de cette étude. Une méthode efficace de purification du molybdène a été mise au point. Les mesures de la concentration intergranulaire de carbone en fonction de la température de carburation et de la teneur volumique ont été effectuées à l'aide d'un spectromè...

  14. A sensitive method for determination of COL-3, a chemically modified tetracycline, in human plasma using high-performance liquid chromatography and ultraviolet detection.

    Science.gov (United States)

    Rudek, Michelle A; Hartke, Carol; Zabelina, Yelena; Zhao, Ming; New, Pamela; Baker, Sharyn D

    2005-04-01

    COL-3, 6-deoxy-6-desmethyl-4-desdimethylamino-tetracycline, is a matrix metalloproteinase inhibitor currently in clinical development. A HPLC-UV method to quantitate COL-3 in human plasma was developed. COL-3 was extracted from plasma using solid-phase extraction cartridges. COL-3 is separated on a Waters Symmetry Shield RP8 (3.9 mm x150 mm, 5 microm) column with EDTA (0.001 M) in sodium acetate (0.01 M, pH 3.5)-acetonitrile mobile phase using a gradient profile at a flow rate of 1 ml/min for 22 min. Carryover was eliminated by using an extended needle wash of methanol:acetonitrile:dichloromethane (1:1:1, v/v/v). Detection of COL-3 and the internal standard, chrysin, was observed at 350 nm. COL-3 and chrysin elute at 8.9 and 9.9 min, respectively. The lower limit of quantitation in human plasma of COL-3 was 75 ng/ml, linearity was observed from 75 to 10,000 ng/ml. A 30,000 ng/ml sample that was diluted 1:50 with plasma was accurately quantitated. This method is rapid, widely applicable, and suitable for quantifying COL-3 in patient samples enabling further clinical pharmacology characterization of COL-3.

  15. DU COTÉ DE CHEZ SOI

    Directory of Open Access Journals (Sweden)

    Marc Smeets

    2013-12-01

    Full Text Available Le voyage en Hollande, au XIXe siècle, s'inscrit dans une longue tradition où résonnent les notions de liberté, tolérance et commerce, mais il acquiert aussi une spécificité étant donné l’importance que prend la figure du « chez soi » : les Pays-Bas, terre d'agrément où le voyageur français se sent à l'aise et où il aimerait, si possible, vivre. Proust, de ce point de vue-là, ne fait rien de neuf quand il rêve dans la Recherche d'une « vie domestique » en terre batave. Les Pays-Bas, pour le voyageur français au XIXe, c'est le home sweet home.

  16. La structure sociale du barreau tunisien dans les années 2000

    Directory of Open Access Journals (Sweden)

    Éric Gobe

    2012-05-01

    Full Text Available L’implication du barreau dans les mouvements protestataires qui ont conduit à la chute du régime de Ben Ali, ne doit pas faire oublier que les avocats tunisiens ne constituent pas une profession unifiée et homogène. L’héritage historique, les « forces du marché », les interventions incessantes du régime autoritaire à travers son appareil répressif ou sous la forme du clientélisme d’État, les inégalités de distribution de capital social, ainsi que la croissance exponentielle du nombre de diplômés de droit ont contribué à fragmenter et à massifier une profession au sein de laquelle les différences de statuts socio-économiques sont désormais patentes. Toutefois, la chute du régime politique de Ben Ali, en déclassant les avocats qui lui étaient acquis, devrait contribuer partiellement à la restructuration de la profession selon de nouveaux critères de segmentation.Social structure of Tunisians lawyers in the 2000’sThe lawyers’ involvement in the protest movements that led to the collapse of Ben Ali’s regime should not make us forget that the Tunisian lawyers are not a unified or homogeneous profession. The historical background, the stakeholders, the continuous interventions of the authoritarian and repressive regime or the State mafia, the inequalities in the distribution of social capital as well as an inflationary number of law graduates have contributed to fragment a profession whose members showed blatant differences in terms of economic status. However, the downfall of Ben Ali’s regime, which was followed by the dismissal of the lawyers that had sided with it, partly contributed to the restructuring of the profession according to new criteria of segmentation.La estructura social del Colégio de Abogados tunecino en los años 2000: la pinza entre la masificación, la mundialización y el régimen político dictatorialLa implicación de los abogados en los movimientos de protesta que han conducido

  17. Cytotoxic evaluation of different fractions of Salvia chorassanica Bunge on MCF-7 and DU 145 cell lines

    Directory of Open Access Journals (Sweden)

    Alireza Golshan

    2016-01-01

    Full Text Available Because of antimicrobial, antioxidant, and anticancer potential, Salvia chorassanica Bunge (Lamiaceae has been considered as a popular herb in Iranian traditional medicine. Previous studies have shown remarkable cytotoxic properties of the methanol, n-hexane and dichloromethane extract of S. chorassanica on human cervical cancer cells. To seek the therapeutic potentials of S. chorassanica, this study was undertaken to evaluate the cytotoxic activities of various extracts of this plant on human breast MCF-7 and prostate cancer DU 145 cells. The DU 145 cells were exposed to different concentrations of plant extracts (1-200 μg/ml. Cytotoxic activities were examined using alamarBlue ® assay and apoptosis was assessed by acridine orange/propodium iodide double staining and evaluation of DNA fragmentation by flow cytometry. Our findings indicated that n-hexane and dichloromethane extracts had more cytotoxic activities against DU 145 and MCF-7 cell lines compared with other extracts (P<0.05. The acridine orange/propodium iodide staining showed apoptogenic properties of n-hexane and dichloromethane extracts which was consequently confirmed by flow cytometric histogram that exhibited an increase in sub-G1 peak in treated cells as compared with untreated cancer cell lines. Taken together, these observations demonstrated cytotoxic effects of S. chorassanica extracts on MCF-7 and DU 145 cell lines which is most likely exerted via apoptosis cell death. Therefore, further investigations on S. chorassanica extracts as potential chemotherapeutic agents are warranted.

  18. Cytotoxic evaluation of different fractions of Salvia chorassanica Bunge on MCF-7 and DU 145 cell lines.

    Science.gov (United States)

    Golshan, Alireza; Amini, Elaheh; Emami, Seyed Ahmad; Asili, Javad; Jalali, Zahra; Sabouri-Rad, Sarvenaz; Sanjar-Mousavi, Naghmeh; Tayarani-Najaran, Zahra

    2016-01-01

    Because of antimicrobial, antioxidant, and anticancer potential, Salvia chorassanica Bunge (Lamiaceae) has been considered as a popular herb in Iranian traditional medicine. Previous studies have shown remarkable cytotoxic properties of the methanol, n-hexane and dichloromethane extract of S. chorassanica on human cervical cancer cells. To seek the therapeutic potentials of S. chorassanica, this study was undertaken to evaluate the cytotoxic activities of various extracts of this plant on human breast MCF-7 and prostate cancer DU 145 cells. The DU 145 cells were exposed to different concentrations of plant extracts (1-200 μg/ml). Cytotoxic activities were examined using alamarBlue(®) assay and apoptosis was assessed by acridine orange/propodium iodide double staining and evaluation of DNA fragmentation by flow cytometry. Our findings indicated that n-hexane and dichloromethane extracts had more cytotoxic activities against DU 145 and MCF-7 cell lines compared with other extracts (P<0.05). The acridine orange/propodium iodide staining showed apoptogenic properties of n-hexane and dichloromethane extracts which was consequently confirmed by flow cytometric histogram that exhibited an increase in sub-G1 peak in treated cells as compared with untreated cancer cell lines. Taken together, these observations demonstrated cytotoxic effects of S. chorassanica extracts on MCF-7 and DU 145 cell lines which is most likely exerted via apoptosis cell death. Therefore, further investigations on S. chorassanica extracts as potential chemotherapeutic agents are warranted.

  19. 1,25-Dihydroxyvitamin D3 inhibition of col1a1 promoter expression in calvariae from neonatal transgenic mice

    Science.gov (United States)

    Bedalov, A.; Salvatori, R.; Dodig, M.; Kapural, B.; Pavlin, D.; Kream, B. E.; Clark, S. H.; Woody, C. O.; Rowe, D. W.; Lichtler, A. C.

    1998-01-01

    We studied the effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on organ cultures of transgenic mouse calvariae containing segments of the Col1a1 promoter extending to -3518, -2297, -1997, -1794, -1763, and -1719 bp upstream of the transcription start site fused to the chloramphenicol acetyltransferase (CAT) reporter gene. 1,25(OH)2D3 had a dose-dependent inhibitory effect on the expression of the -3518 bp promoter construct (ColCAT3.6), with maximal inhibition of about 50% at 10 nM. This level of inhibition was consistent with the previously observed effect on the endogenous Col1a1 gene in bone cell models. All of the shorter constructs were also inhibited by 10 nM 1,25(OH)2D3, suggesting that the sequences required for 1, 25(OH)2D3 inhibition are downstream of -1719 bp. The inhibitory effect of 1,25(OH)2D3 on transgene mRNA was maintained in the presence of the protein synthesis inhibitor cycloheximide, suggesting that the inhibitory effect on Col1a1 gene transcription does not require de novo protein synthesis. We also examined the in vivo effect of 1,25(OH)2D3 treatment of transgenic mice on ColCAT activity, and found that 48 h treatment caused a dose-dependent inhibition of CAT activity in calvariae comparable to that observed in organ cultures. In conclusion, we demonstrated that 1,25(OH)2D3 inhibits Col1A1 promoter activity in transgenic mouse calvariae, both in vivo and in vitro. The results indicate that there is a 1, 25(OH)2D3 responsive element downstream of -1719 bp. The inhibitory effect does not require new protein synthesis.

  20. Transferências culturais entre metrópole e colônia: o livro escolar como instrumento da formação do cidadão em Quebec no século 19

    Directory of Open Access Journals (Sweden)

    Thérèse Hamel

    2012-06-01

    Full Text Available  Este artigo pretende mostrar como se transforma um manual escolar criado primeiramente em uma metrópole (a França, para, em seguida, ser utilizado numa ex-colônia (o Canadá, onde o contexto social, político, ideológico e religioso difere em vários aspectos. Mesmo após o descobrimento francês, a conquista inglesa e muito depois da independência do Canadá em relação à Inglaterra, em 1867, a província de Quebec manteve, durante muito tempo, relações privilegiadas com a França. As idéias pedagógicas, o pessoal escolar e mesmo os livros didáticos atravessavam o oceano até a ex-colônia. Foi assim que uma gramática utilizada durante muito tempo na França laica, no fim do século passado, tornou-se um manual oficial utilizado em Quebec, onde, ao contrário da França, o poder da Igreja Católica nas orientações escolares era preponderante.Palavras-chave: manual escolar, Quebec.   Résume Cet article veut montrer se transforme un manuel scolaire d'abord conçu dans une métropole (Ia France, pour ensuite être utilisé dans une ancienne colonie (le Canada, ou le contexte social, politique, idéologique et religieux diftere sur plusieurs aspects. Même apres Ia découverte française, Ia conquête anglaise et bien aprés I'indépendance du Canada par rapport à l' Angleterre em 1867, Ia province de Québec à longtemps maintenu des relations privilégiées avec Ia France. Les idées pédagogiques, le personnel scolaire et même les livres didactiques traversaient I'océan jusqu'à l’áncienne colonie. C'est ainsi qu'une grammaire longtemps utilisée dans Ia France laique, à Ia fin du siecle dernier, est devenue un manuel officiel utilisé au Québec, oú contrairement à Ia France, le pouvoir de l’Église Catholique sur les orientations scolaires était prépondérant. Mots-clé: manuel scolaire, Québec. 

  1. A novel missense mutation of COL5A2 in a patient with Ehlers–Danlos syndrome

    Science.gov (United States)

    Watanabe, Miki; Nakagawa, Ryuji; Naruto, Takuya; Kohmoto, Tomohiro; Suga, Ken-ichi; Goji, Aya; Kagami, Shoji; Masuda, Kiyoshi; Imoto, Issei

    2016-01-01

    Ehlers–Danlos syndrome (EDS) is a group of inherited connective tissue disorders characterized by hyperextensible skin, joint hypermobility and soft tissue fragility. For molecular diagnosis, targeted exome sequencing was performed on a 9-year-old male patient who was clinically suspected to have EDS. The patient presented with progressive kyphoscoliosis, joint hypermobility and hyperextensible skin without scars. Ultimately, classical EDS was diagnosed by identifying a novel, mono-allelic mutation in COL5A2 [NM_000393.3(COL5A2_v001):c.682G>A, p.Gly228Arg]. PMID:27656288

  2. A novel missense mutation of COL5A2 in a patient with Ehlers-Danlos syndrome.

    Science.gov (United States)

    Watanabe, Miki; Nakagawa, Ryuji; Naruto, Takuya; Kohmoto, Tomohiro; Suga, Ken-Ichi; Goji, Aya; Kagami, Shoji; Masuda, Kiyoshi; Imoto, Issei

    2016-01-01

    Ehlers-Danlos syndrome (EDS) is a group of inherited connective tissue disorders characterized by hyperextensible skin, joint hypermobility and soft tissue fragility. For molecular diagnosis, targeted exome sequencing was performed on a 9-year-old male patient who was clinically suspected to have EDS. The patient presented with progressive kyphoscoliosis, joint hypermobility and hyperextensible skin without scars. Ultimately, classical EDS was diagnosed by identifying a novel, mono-allelic mutation in COL5A2 [NM_000393.3(COL5A2_v001):c.682G>A, p.Gly228Arg].

  3. Actividad Antioxidante de extractos de Calendula officinalis asociados a soportes de colágeno tipo I

    OpenAIRE

    Flórez Cabrera, Adriana Matilde

    2014-01-01

    En este trabajo se evaluó la actividad antioxidante de los metabolitos secundarios presentes en dos extractos, oleoso e hidroglicólico, de flores de Calendula officinalis liberados de forma controlada por soportes de colágeno tipo I con micropartículas de gelatina-colágeno desarrollados mediante ingeniería de tejidos, con el fin determinar su posible aplicación en heridas cutáneas crónicas con elevadas concentraciones de especies reactivas de oxígeno. Se realizaron pruebas de citotoxicida...

  4. Early stage breast cancer: Is exclusive radiotherapy an option for early breast cancers with complete clinical response after neo-adjuvant chemotherapy?; Cancers du sein de stade II-IIIA: la radiotherapie exclusive est-elle une option en cas de reponse clinique complete a la chimiotherapie neoadjuvante?

    Energy Technology Data Exchange (ETDEWEB)

    Daveau, C.; Abrous-Anane, S.; Kirova, Y.M.; Dendale, R.; Campana, F.; Fourquet, A.; Bollet, M.A. [Departement de radiotherapie, institut Curie, 26, rue d' Ulm, 75005 Paris (France); Savignoni, A.; Gautier, C. [Departement de biostatistiques, institut Curie, 26, rue d' Ulm, 75005 Paris (France); Pierga, J.Y. [Departement d' oncologie, medicale, institut Curie, 26, rue d' Ulm, 75005 Paris (France); Reyal, F. [Departement de chirurgie, institut Curie, 26, rue d' Ulm, 75005 Paris (France)

    2011-04-15

    Purpose. - To determine whether exclusive radiotherapy could be a therapeutic option after complete clinical response (cCR) to neo-adjuvant chemotherapy (NCT) for early breast cancers (EBC). Patients and methods. - Between 1985 and 1999, 1477 patients received neo-adjuvant chemotherapy for early breast cancer considered to be too large for primary conservative surgery. Of 165 patients with complete clinical response, 65 were treated by breast surgery (with radiotherapy) and 100 by exclusive radiotherapy. Results. - The two groups were comparable in terms of baseline characteristics, except for larger initial tumor sizes in the exclusive radiotherapy group. There were no significant differences in overall, disease-free and metastasis-free survivals. Five-year and 10-year overall survivals were 91 and 77% in the no surgery group and 82 and 79% in the surgery group, respectively (P = 0.9). However, a non-significant trend towards higher locoregional recurrence rates (LRR) was observed in the no surgery group (31 vs. 17% at 10 years; P = 0.06). In patients with complete responses on mammography and/or ultrasound, LRR were not significantly different (P = 0.45, 10-year LRR: 21 in surgery vs. 26% in exclusive radiotherapy). No significant differences were observed in terms of the rate of cutaneous, cardiac or pulmonary toxicities. Conclusion. - Surgery is a key component of locoregional treatment for breast cancers that achieved complete clinical response to neo-adjuvant chemotherapy. (authors)

  5. Prognosis of Lung Cancer: Heredity or Environment

    Science.gov (United States)

    2015-06-01

    Service Career Development Award (10–024), and a Department of Defense Early Investigator Synergistic Idea Award (W18XWH-12-1-0544). The authors...Nurgalieva Z, Du XL. Racial dis- parities and survival for nonsmall-cell lung cancer in a large cohort of black and white elderly patients. Cancer 2009;115

  6. Cirque du Monde as a health intervention

    Science.gov (United States)

    Fournier, Cynthia; Drouin, Mélodie-Anne; Marcoux, Jérémie; Garel, Patricia; Bochud, Emmanuel; Théberge, Julie; Aubertin, Patrice; Favreau, Gil; Fleet, Richard

    2014-01-01

    Abstract Objective To present Cirque du Soleil’s social circus program, Cirque du Monde, to explore its potential as a primary health care tool for family physicians. Data sources A review of the literature in PubMed, the Cochrane Library, PsycINFO, LaPresse, Eureka, Google Scholar, and Érudit using the key words circus, social circus, Cirque du Monde, and Cirque du Soleil; a Montreal-based initiative, Espace Transition, modeled on Cirque du Monde; and personal communication with Cirque du Soleil’s Social Circus Training Advisor. Study selection The first 50 articles or websites identified for each key word in each of the databases were examined on the basis of their titles and abstracts in the case of articles, and on the basis of their titles and page content in the case of websites. Articles and websites that explored an aspect of social circuses or that described an intervention that involved circuses were then retained for analysis. Because all literature on social circuses was searched, no criterion for year of publication was used. Synthesis No articles on the social circus as a health intervention were found. One study on the use of the circus as an intervention in schools was identified. It demonstrated an increase in self-esteem in the children who took part. One study on the use of the circus in a First Nations community was found; it contained nonspecific, qualitative findings. The other articles identified were merely descriptions of social circuses. One website was identified on the use of the social circus to help youth who had been treated in a hospital setting for major psychiatric disorders to re-enter the community. The team in the pediatric psychiatry department at Centre Hospitalier Universitaire Sainte-Justine, the children’s hospital in Montreal, Que, was contacted; they were leading this project, called Espace Transition. The unpublished preliminary findings of its pilot project demonstrate substantial improvements in overall patient

  7. Influence du pH, d'acides aminés et de diverses substances organiques sur la fongitoxicité du pyriméthanil, du glufosinate, du captafol, du cymoxanil et du fenpiclonil vis-à-vis de certaines souches de Botrytis cinerea

    OpenAIRE

    1994-01-01

    L’effet du pyriméthanil, du fenpiclonil, du captafol, du glufosinate et du cymoxanil sur l’élongation des filaments germinatifs des spores de Botrytis cinerea est étudié dans diverses conditions culturales du champignon. Si le fenpiclonil présente un niveau d’activité stable entre pH 3,6 et 7,3, en revanche les autres pesticides voient leur toxicité chuter dans des milieux acides (ex pyriméthanil) ou neutres à faiblement basiques (ex captafol, cymoxanil, glufosinate). L’adjonction d’extrait d...

  8. L’internationalisation du roman arabe

    Directory of Open Access Journals (Sweden)

    Dr Dalia Khraibani

    2016-09-01

    Full Text Available La littérature arabe est presque invisible dans l’espace littéraire mondiale pour des raisons diverses. Pourtant, des écrivains réussissent à s’imposer sur la scène littéraire internationale et leurs œuvres deviennent des chefs d’œuvre de la littérature mondiale. Parmi ces écrivains, nous citons Tayeb Saleh qui connaît un succès international surtout avec la parution de son second roman "Saison de migration vers le Nord". Notre étude vise à montrer comment ce roman par son contenu et sa forme, occupe une place de choix dans la littérature mondiale. En effet, nous allons examiner le contexte historique de l’émergence du roman qui joue un rôle dans sa diffusion au niveau mondial. L’une des clés de réussite du roman est la technique narrative qui retient l’haleine du lecteur dès la première page. De plus, le roman répond à l’horizon d’attente du lectorat occidental en représentant des images particulières de la société arabo-africaine qui correspondent aux stéréotypes et clichés du public occidental vis-à-vis des Arabes.

  9. Établissements recevant du public

    CERN Document Server

    2014-01-01

    Synthèse pour les installations électriques, des règles de sécurité contre les risques d’incendie et de panique dans les établissements recevant du public (ERP), selon l’arrêté du 25 juin 1980 modifié. L'ouvrage traite à la fois des dispositions communes aux établissements du 1er groupe (1ère à 4ème catégorie), du 2ème groupe (5ème catégorie) et des règles particulières applicables aux différents types d'établissements (structures d'accueil pour personnes âgées ou handicapées, hôtels, magasins de vente, restaurants et débit de boisson, établissements de soins, administration, châpiteaux, etc...) Sont ainsi notamment traités, dans le cadre des dispositions communes aux établissements du 1er groupe, pour les installations électriques normales : les installations des appareils, tableaux et canalisations, les locaux électriques et installation de machines, l'éclairage normal des locaux accessibles au public, le chauffage et la ventilation, l'installation d'eau chaude sanita...

  10. De Gainsborough à Turner : l'âge d'or du paysage et du portrait anglais dans les collections du musée du Louvre

    OpenAIRE

    Adrien, Muriel

    2015-01-01

    L’exposition De Gainsborough à Turner : l'âge d'or du paysage et du portrait anglais dans les collections du musée du Louvre co-organisée par le Musée du Louvre, le Musée d’Art et d’Archéologie de Valence et le Musée des Beaux-arts de Quimper présentait une sélection de 35 peintures de la collection du Louvre, complétée par 38 dessins du département des Arts Graphiques. Les commissaires de l'exposition étaient : Guillaume Faroult, conservateur en charge des peintures françaises du 18ème siècl...

  11. COOH-terminal collagen Q (COLQ) mutants causing human deficiency of endplate acetylcholinesterase impair the interaction of ColQ with proteins of the basal lamina.

    Science.gov (United States)

    Arredondo, Juan; Lara, Marian; Ng, Fiona; Gochez, Danielle A; Lee, Diana C; Logia, Stephanie P; Nguyen, Joanna; Maselli, Ricardo A

    2014-05-01

    Collagen Q (ColQ) is a key multidomain functional protein of the neuromuscular junction (NMJ), crucial for anchoring acetylcholinesterase (AChE) to the basal lamina (BL) and accumulating AChE at the NMJ. The attachment of AChE to the BL is primarily accomplished by the binding of the ColQ collagen domain to the heparan sulfate proteoglycan perlecan and the COOH-terminus to the muscle-specific receptor tyrosine kinase (MuSK), which in turn plays a fundamental role in the development and maintenance of the NMJ. Yet, the precise mechanism by which ColQ anchors AChE at the NMJ remains unknown. We identified five novel mutations at the COOH-terminus of ColQ in seven patients from five families affected with endplate (EP) AChE deficiency. We found that the mutations do not affect the assembly of ColQ with AChE to form asymmetric forms of AChE or impair the interaction of ColQ with perlecan. By contrast, all mutations impair in varied degree the interaction of ColQ with MuSK as well as basement membrane extract (BME) that have no detectable MuSK. Our data confirm that the interaction of ColQ to perlecan and MuSK is crucial for anchoring AChE to the NMJ. In addition, the identified COOH-terminal mutants not only reduce the interaction of ColQ with MuSK, but also diminish the interaction of ColQ with BME. These findings suggest that the impaired attachment of COOH-terminal mutants causing EP AChE deficiency is in part independent of MuSK, and that the COOH-terminus of ColQ may interact with other proteins at the BL.

  12. A receptor tyrosine kinase, UFO/Axl, and other genes isolated by a modified differential display PCR are overexpressed in metastatic prostatic carcinoma cell line DU145.

    Science.gov (United States)

    Jacob, A N; Kalapurakal, J; Davidson, W R; Kandpal, G; Dunson, N; Prashar, Y; Kandpal, R P

    1999-01-01

    We have used a modified differential display PCR protocol for isolating 3' restriction fragments of cDNAs specifically expressed or overexpressed in metastatic prostate carcinoma cell line DU145. Several cDNA fragments were identified that matched to milk fat globule protein, UFO/Axl, a receptor tyrosine kinase, human homologue of a Xenopus maternal transcript, laminin and laminin receptor, human carcinoma-associated antigen, and some expressed sequence tags. The transcript for milk fat globule protein, a marker protein shown to be overexpressed in breast tumors, was elevated in DU145 cells. The expression of UFO/Axl, a receptor tyrosine kinase, was considerably higher in DU145 cells as compared to normal prostate cells and prostatic carcinoma cell line PC-3. The overexpression of UFO oncogene in DU145 cells is discussed in the context of prostate cancer metastasis.

  13. Management of choledochal cyst Tratamento do cisto de colédoco

    Directory of Open Access Journals (Sweden)

    Orlando Jorge Martins Torres

    2007-09-01

    Full Text Available BACKGROUND: Choledochal cysts are rare diseases in adults. The risk of malignant transformation is well documented to be age-related and early complete cyst excision is currently advocated. Type IVa cyst management is more controversial. Extensive intrahepatic involvement of the biliary system may preclude complete excision. AIM: The aim of this study was to present a case of type IVa choledochal cyst in a young female patient. CASE REPORT: An 18-year-old female, complaining of right upper quadrant pain, nausea, vomiting, fever, and jaundice was admitted. The diagnosis of type IVa choledochal cyst was made by computerized tomography. The patient was underwent cholecystectomy, excision of the anterior wall of the cyst, and Roux-en-Y hepaticojejunostomy. No postoperative complication was noted and the patient was discharged five days after operation. CONCLUSION: Total cyst excision is the ideal treatment of choledochal cysts in adults. However, in IVa type, due to the extensive intrahepatic involvement of the biliary system, the anatomy of the cyst, and the surgeon's judgment will determine the surgical approach taken for the patient.RACIONAL: A doença de cistos de colédoco em adultos é rara. O risco de transformação maligna é bem documentado, sendo relacionado a idade, além da completa excisão do cisto ser aconselhada. O manuseio e gestão do cisto tipo IVa gera maior controvérsia. O envolvimento intra-hepático extensivo do sistema biliar pode prevenir a sua completa excisão. OBJETIVOS: Relatar o caso de um cisto de colédoco do tipo IVa em paciente feminina. RELATO DE CASO: Paciente feminina, 18 anos de idade, foi admitida com queixa de dor no quadrante superior direito, náusea, vômitos, febre e icterícia. O diagnóstico de cisto de colédoco do tipo IVa foi realizado através de tomografia computadorizada. A paciente foi submetida à colecistectomia, excisão da parede anterior do cisto e hepatojejunostomia em Y-de-Roux. Nenhuma

  14. Detecten Hepatozoon sp. en una colònia felina de Barcelona

    OpenAIRE

    Ortuño Romero, Anna M.

    2009-01-01

    En un estudi realitzat pel Departament de Sanitat i Anatomia Animals de la Facultat de Veterinària de la UAB, amb el suport del Servei de Salut Pública de la Diputació de Barcelona, s’ha detectat la presència d’Hepatozoon sp. en colònies de gats de carrer. Un fet important donat que és el primer cop que aquests paràsits intracel·lulars són identificats en felins del nord d’Espanya, i perquè, a més a més, evidencia la importància d’establir programes sanitaris que permetin detectar i controlar...

  15. Mutation-based growth charts for SEDC and other COL2A1 related dysplasias

    DEFF Research Database (Denmark)

    Terhal, Paulien A; van Dommelen, Paula; Le Merrer, Martine

    2012-01-01

    patients with glycine substitutions in the triple-helical domain of COL2A1. In addition, measurements of 27 patients with other molecular defects, such as arginine to cysteine substitutions, splice mutations, and mutations in the C-terminal propeptide have been plotted on the chart. Height of the patients...... progressively deviate from that of normal children: compared to normal WHO charts, the mean length/height is -2.6 SD at birth, -4.2 SD at 5 years, and -5.8 SD in adulthood. The mean adult height (male and female combined) of patients with glycine substitutions in the triple-helical region is 138.2 cm...... with amino-terminal substitutions, while patients with splice mutations are relatively tall. However, there are exceptions and specific mutations can have a strong or a relatively mild negative effect on growth. The observation of significant difference in adult height between affected members of the same...

  16. Investigation on Painting Materials in "Madonna col Bambino e S. Giovannino" by Botticelli

    Science.gov (United States)

    Bersani, D.; Lottici, P. P.; Casoli, A.; Ferrari, M.; Lottini, S.; Cauzzi, D.

    A study on the painting materials (pigments and binders) of the famous painting "Madonna col Bambino e S. Giovannino" by Sandro Botticelli, located in the Museo Civico of Piacenza (Italy), was performed before a recent restoration. The painting materials were investigated by the analysis of five millimetric samples taken in damaged regions. The pigments were determined using the micro-Raman spectroscopy, with the 632.8nm line of a He-Ne laser. Despite the strong fluorescence background, the nature of the ground layer (gypsum and anhydrite) and of most pigments (i.e. goethite, lapis lazuli, white lead) was determined. Gas chromatography coupled with mass spectroscopy (GC/MS) was used to determine the organic binder media, and in particular proteinaceous and lipid materials. Egg and animal glue were found, while no siccative oils were detected.

  17. Analyse énonciative du point du vue, narration et analyse de discours

    Directory of Open Access Journals (Sweden)

    Alain Rabatel

    2007-01-01

    Full Text Available Cet article montre que l’analyse énonciative du point de vue (PDV, en rupture avec la typologie des focalisations de Genette, peut renouveler partiellement la narratologie, à la condition de substituter à l’approche immanentiste du récit une analyse interactionnelle de la narration. L’article présente d’abord l’approche énonciative du PDV, en appui des théories de Ducrot, et, sur cette base, propose diverses modalités de PDV (représenté, raconté, asserté qui donnent corps au point de vue des personnages ou du narrateur en modifiant sensiblement les analyses de Genette. Dans une deuxième partie, l’article envisage le rôle des PDV dans la narration, notamment dans la réévaluation des dimensions cognitive et pragmatique de la mimésis, puis dans les mécanismes inférentiels-interprétatifs, proches du système de sympathie de Jouve, enfin, dans la revalorisation du rôle du narrateur •puisque ce dernier se construit dans le même temps qu’il construit ses personnages.

  18. Aberrations of 6q13 mapped to the COL12A1 locus in chondromyxoid fibroma.

    Science.gov (United States)

    Yasuda, Taketoshi; Nishio, Jun; Sumegi, Janos; Kapels, Kayla M; Althof, Pamela A; Sawyer, Jeffrey R; Reith, John D; Bridge, Julia A

    2009-11-01

    Chondromyxoid fibroma, a rare benign bone tumor, may be mistaken for chondrosarcoma. Although cytogenetic studies of chondromyxoid fibroma are few, rearrangements of the long arm of chromosome 6, frequently expressed as an inv(6)(p25q13), are prominent. In this study, conventional cytogenetic analysis of 16 chondromyxoid fibroma samples from 14 patients revealed rearrangements of chromosome 6 in 10 of 11 clonally abnormal specimens. In addition to 6q13 rearrangements, recurrent 6p25 and 6q25 anomalies were detected. Notably, an identical t(6;9)(q25;q22) translocation was identified in two cases, suggesting that it represents a distinct translocation of chondromyxoid fibroma. In an effort to further define the aberrant 6q13 breakpoint and identify the molecular consequences, a fluorescence in situ hybridization (FISH)-based positional cloning strategy on chondromyxoid fibroma abnormal metaphase and interphase cells using a series of bacterial and plasmid artificial chromosome (BAC/PAC) probe combinations spanning a 6.1 Mb region was employed. The breakpoint on 6q13 was located within the COL12A1 gene, a collagen gene purportedly involved in another benign bone tumor, subungual exostosis. The findings of this study expand our knowledge of chromosomal alterations in chondromyxoid fibroma, identify COL12A1 as the likely gene candidate within the recurrent 6q13 breakpoint, and provide an alternative approach for detecting 6q13 anomalies in nondividing cells of chondromyxoid fibroma. The latter could potentially be utilized as an adjunct in diagnostically challenging cases.

  19. 一成骨不全家系的COL1A1基因突变检测%Mutation Detection of COL1A1 Gene in a Pedigree with Osteogenesis Imperfecta

    Institute of Scientific and Technical Information of China (English)

    秦炜; 何隽祥; 施瑾; 邢清和; 高建军; 钱学庆; 刘壮俊; 舒安利; 贺林

    2005-01-01

    成骨不全(Osteogenesis imperfecta,OI)是一种由于Ⅰ型胶原形成障碍,导致骨脆性增强为主要症状的常染色体显性遗传性疾病.临床上主要表现为骨质脆弱、蓝巩膜、耳聋和中等程度的关节畸形等症状.成骨不全基因分别定位于17q21.31-q22和7q22.1,其致病基因分别为COL1A1和COL1A2.对一常染色体显性遗传的成骨不全家系进行连锁分析,在COL1A1遗传位点发现紧密连锁(LOD=9.31;θ=.00).突变检测发现在COL1A1基因第26内含子5′端剪接位点处存在一由GT转换为AT的致病突变,该突变引起的异常剪接是导致成骨不全的致病原因之一.%Osteogenesis imperfecta(OI)is heritable bone fragility,which is inherited as an autosomal dominant trait clinical presentation.Clinical symptom,in general,is dominantly inherited OI with blue sclerae,hearing loss and mild-moderate skeletal deformity.Genetic loci of OI have been mapped to17q21.31-q22 and 7q22.1,in which COL1A1 and COL1A2 are known to be the causal genes.In this work,we performed linkage analysis in a kindred with autosomal dominant hereditary OI.A tight linkage to the markers on chromosome 17q21.31-q22 (maximum two-point lod score:9.31 at θ=.00) was observed.Sequence analysis of COL1A1 revealed a single-base mutation that converted the consensus sequence at the 5′end of intron 26 from GT to AT to form an abnormal splicing site leading to OI.

  20. Les métamorphoses du pouvoir

    OpenAIRE

    Adler, Alfred

    2004-01-01

    On sait que la sorcellerie en Afrique peut s'exercer du faible au fort ou l'inverse. Les situations qui sont ici analysées sont celles où les rois ou les chefs sont expressément désignés comme sorciers, soit qu'ils le deviennent du fait même de leur investiture, soit qu'il leur ait fallu le devenir pour accéder au pouvoir et le conserver face à des rivaux usant des mêmes moyens. Quoi qu'il en soit, un roi-sorcier offre une figure paradoxale du pouvoir, et cela doublement : garant de l'...

  1. Frequency of COL4A3/COL4A4 mutations amongst families segregating glomerular microscopic hematuria and evidence for activation of the unfolded protein response. Focal and segmental glomerulosclerosis is a frequent development during ageing.

    Directory of Open Access Journals (Sweden)

    Louiza Papazachariou

    Full Text Available Familial glomerular hematuria(s comprise a genetically heterogeneous group of conditions which include Alport Syndrome (AS and thin basement membrane nephropathy (TBMN. Here we investigated 57 Greek-Cypriot families presenting glomerular microscopic hematuria (GMH, with or without proteinuria or chronic kidney function decline, but excluded classical AS. We specifically searched the COL4A3/A4 genes and identified 8 heterozygous mutations in 16 families (28,1%. Eight non-related families featured the founder mutation COL4A3-p.(G1334E. Renal biopsies from 8 patients showed TBMN and focal segmental glomerulosclerosis (FSGS. Ten patients (11.5% reached end-stage kidney disease (ESKD at ages ranging from 37-69-yo (mean 50,1-yo. Next generation sequencing of the patients who progressed to ESKD failed to reveal a second mutation in any of the COL4A3/A4/A5 genes, supporting that true heterozygosity for COL4A3/A4 mutations predisposes to CRF/ESKD. Although this could be viewed as a milder and late-onset form of autosomal dominant AS, we had no evidence of ultrastructural features or extrarenal manifestations that would justify this diagnosis. Functional studies in cultured podocytes transfected with wild type or mutant COL4A3 chains showed retention of mutant collagens and differential activation of the unfolded protein response (UPR cascade. This signifies the potential role of the UPR cascade in modulating the final phenotype in patients with collagen IV nephropathies.

  2. OsCOL10, a CONSTANS-Like Gene, Functions as a Flowering Time Repressor Downstream of Ghd7 in Rice.

    Science.gov (United States)

    Tan, Junjie; Jin, Mingna; Wang, Jiachang; Wu, Fuqing; Sheng, Peike; Cheng, Zhijun; Wang, Jiulin; Zheng, Xiaoming; Chen, Liping; Wang, Min; Zhu, Shanshan; Guo, Xiuping; Zhang, Xin; Liu, Xuanming; Wang, Chunming; Wang, Haiyang; Wu, Chuanyin; Wan, Jianmin

    2016-04-01

    Flowering time, or heading date, is a critical agronomic trait that determines the cropping season and regional adaptability, and ultimately grain yield in rice. A number of genes involved in photoperiodic flowering have been cloned and their roles in modulating expression of the flowering genes have been characterized to a certain extent. However, much less is known about the pathway in transmitting the day length response signal(s) to induce transition to reproductive growth. Here, we report a constitutive flowering repressor OsCOL10, which encodes a member of the CONSTANS-like (COL) family. Transgenic rice plants overexpressing OsCOL10 (driven by a strong promoter or by fusing it to the activation domain of VP64) showed delayed flowering time under both short and long days.OsCOL10 is affected by the circadian clock and is preferentially expressed in leaf mesophyll cells; it is localized to the nucleus and has transcriptional activation activity. Further studies show that OsCOL10 represses the expression of theFT-like genes RFT1 and Hd3a through Ehd1. Transcripts of OsCOL10 are more abundant in plants carrying a functional Ghd7 allele or overexpressing Ghd7 than in Ghd7-deficient plants, thus placing OsCOL10 downstream of Ghd7.Taking these findings together, we conclude that OsCOL10 functions as a flowering time repressor that links Ghd7 and Ehd1 in rice.

  3. EHLERS-DANLOS SYNDROME TYPE-IV - PHENOTYPIC CONSEQUENCES OF A SPLICING MUTATION IN ONE COL3A1 ALLELE

    NARCIS (Netherlands)

    SILLENCE, DO; CHIODO, AA; CAMPBELL, PE; COLE, WG

    1991-01-01

    The features of a child with Ehlers-Danlos syndrome type IV (EDS IV) resulting from a mutation in one COL3A1 allele were studied. The child was heterozygous for a G- to A-transition at the splice donor site of intron 41. It resulted in the splicing out of the exon 41 encoded sequence from alpha-1(II

  4. Mutations in the codon for a conserved arginine-1563 in the COL4A5 collagen gene in Alport syndrome

    DEFF Research Database (Denmark)

    Zhou, J; Gregory, M C; Hertz, Jens Michael

    1993-01-01

    We have screened 110 unrelated Alport syndrome kindreds for mutations in the exon 48 region of the COL4A5 collagen gene. Denaturing gradient gel electrophoresis (DGGE) of the PCR-amplified region of exon 48 revealed sequence variants in DNA from affected males and carriers of three unrelated kind...

  5. Asymptomatic parental mosaicism for osteogenesis imperfect associated with a new splice site mutation in COL1A2

    DEFF Research Database (Denmark)

    Frederiksen, Anja Lisbeth; Dunø, Morten; Johnsen, Iben Birgit Gade;

    2016-01-01

    Recurrent lethal perinatal osteogenesis imperfecta may result from asymptomatic parental mosaicism. A previously unreported mutation in COL1A2 leads to recurrent cases of fetal osteogenesis imperfecta Sillence type IIA, which emphasizes the importance of clinical and genetic evaluation of mosaicism...... in asymptomatic parents as verified mosaicism highly increases recurrence risk....

  6. Asymptomatic parental mosaicism for osteogenesis imperfecta associated with a new splice site mutation in COL1A2

    OpenAIRE

    2016-01-01

    Key Clinical Message Recurrent lethal perinatal osteogenesis imperfecta may result from asymptomatic parental mosaicism. A previously unreported mutation in COL1A2 leads to recurrent cases of fetal osteogenesis imperfecta Sillence type IIA, which emphasizes the importance of clinical and genetic evaluation of mosaicism in asymptomatic parents as verified mosaicism highly increases recurrence risk.

  7. Autosomal dominant rhegmatogenous retinal detachment associated with an Arg453Ter mutation in the COL2A1 gene.

    NARCIS (Netherlands)

    Go, S.L.; Maugeri, A.; Mulder, J.J.S.; Driel, M.A. van; Cremers, F.P.M.; Hoyng, C.B.

    2003-01-01

    PURPOSE: To investigate the clinical features and molecular causes of autosomal dominant rhegmatogenous retinal detachment (RRD) in two large families. METHODS: Clinical examination and linkage analysis of both families using markers flanking the COL2A1 gene associated with Stickler syndrome type 1,

  8. Molecular characterization of CONSTANS-Like (COL) genes in banana (Musa acuminata L. AAA Group, cv. Grand Nain).

    Science.gov (United States)

    Chaurasia, Akhilesh Kumar; Patil, Hemant Bhagwan; Azeez, Abdul; Subramaniam, Vadakanthara Ramakrishnan; Krishna, Bal; Sane, Aniruddha Prafullachandra; Sane, Prafullachandra Vishnu

    2016-01-01

    The CONSTANS (CO) family is an important regulator of flowering in photoperiod sensitive plants. But information regarding their role in day neutral plants is limited. We report identification of nine Group I type CONSTANS-like (COL) genes of banana and their characterization for their age dependent, diurnal and tissue-specific expression. Our studies show that the Group I genes are conserved in structure to members in other plants. Expression of these genes shows a distinct circadian regulation with a peak during light period. Developmental stage specific expression reveals high level transcript accumulation of two genes, MaCOL3a and MaCOL3b, well before flowering and until the initiation of flowering. A decrease in their transcript levels after initiation of flowering is followed by an increase in transcription of other members that coincides with the continued development of the inflorescence and fruiting. CO binding cis-elements are observed in at least three FT -like genes in banana suggesting possible CO-FT interactions that might regulate flowering. Distinct tissue specific expression patterns are observed for different family members in mature leaves, apical inflorescence, bracts, fruit skin and fruit pulp suggesting possible roles other than flowering. This is the first exhaustive study of the COL genes belonging to Group I of banana.

  9. Mobilization properties of small ColE1-like plasmids carrying kanamycin resistance gene isolated from Salmonella enterica serotypes

    Science.gov (United States)

    Background: Previously we isolated and characterized various groups of small kanamycin resistance (KanR) ColE1-like plasmids from different serotypes of Salmonella enterica isolates. These plasmids all carried the aph(3)-I gene encoding the aminoglycoside phosphotransferase responsible for the kanam...

  10. Localized and generalized forms of blistering in junctional epidermolysis bullosa due to COL17A1 mutations in the Netherlands

    NARCIS (Netherlands)

    Pasmooij, A. M. G.; Pas, H. H.; Jansen, G. H. L.; Lemmink, H. H.; Jonkman, M. F.

    2007-01-01

    Background Mutations in the gene COL17A1 coding for type XVII collagen cause non-Herlitz junctional epidermolysis bullosa (nH-JEB). Objectives Here we give an overview of the genotype-phenotype correlation in 12 patients from the Netherlands with type XVII collagen-deficient nH-JEB. Patient and meth

  11. One Novel Frameshift Mutation on Exon 64 of COL7A1 Gene in an Iranian Individual Suffering Recessive Dystrophic Epidermolysis Bullosa.

    Science.gov (United States)

    Khaniani, Mahmoud Shekari; Sohrabi, Nasrin; Derakhshan, Neda Mansoori; Derakhshan, Sima Mansoori

    2015-01-01

    Recessive dystrophic epidermolysis bullosa (RDEB) is an extremely rare subtype of bullous dermatosis caused by the COL7A1 gene mutation. After genomic DNA extraction from the peripheral blood sample of all subjects (3 pedigree members and 3 unrelated control individuals), COL7A1 gene screening was performed by PCR amplification and direct DNA sequencing of all of the coding exons and flanking intronic regions. Genetic analysis of the COL7A1 gene in an affected individual revealed a novel mutation: c.5493delG (p.K1831Nfs*10) in exon 64 of the COL7A1 gene in homozygous state. This mutation was not discovered in 3 unrelated Iranian control individuals. These data suggest that c.5493delG may influence the phenotype of RDEB. The result of this case report contributes to the expanding database on COL7A1 mutations.

  12. Le Flaubert de Charles Du Bos

    Directory of Open Access Journals (Sweden)

    Jacques Neefs

    2009-01-01

    Full Text Available Charles Du Bos a porté une attention constante à l’œuvre de Flaubert (à l’exclusion de Bouvard et Pécuchet qui semble ne pas exister pour lui, à Madame Bovary et à L’Éducation sentimentale en particulier. La mise en relation de son étude : « Sur le milieu intérieur chez Flaubert », écrite en 1921, avec des textes du Journal de 1923 et de 1937, les rapprochements avec Gogol, Thomas Hardy, Tolstoï, Baudelaire, Henry James qui traversent les écrits de Du Bos, permettent de suivre ce que celui-ci décrit comme « l’expérience spirituelle » d’une matérialité comprise dans la conquête de la triple exigence du Beau, du Vivant et du Vrai. Du Bos décèle la force de l’œuvre de Flaubert dans la « disproportion » du style, et dans la puissance d’absorption qui fait la densité de cette prose, et qui désigne un extraordinaire travail de conversion. L’obscure expérience spirituelle ainsi poursuivie est celle d’un absolu de l’art, expérience paradoxale d’un « mystique qui ne croit à rien » (comme se désignait Flaubert lui-même, que le critique lie à une interrogation sur sa propre conversion.Charles Du Bos devoted an unflagging attention to Flaubert’s work (except for Bouvard et Pécuchet, which, apparently, according to him did not exist, to Madame Bovary and in particular L’Éducation sentimentale. The connection between his essay “Sur le milieu intérieur chez Flaubert”, written in 1921, and extracts from his Journal, from 1923 to 1937, the comparisons with Gogol, Thomas Hardy, Tolstoy, Baudelaire, and Henry James that run through the writings of Du Bos, allow us to follow what he terms “the spiritual experience” of a materiality encompassed in the conquest of the triple demand of the Beautiful, the Living, the Truth. Du Bos detects the power of Flaubert’s work in the “disproportion” of his style, and the power of absorption that forms the density of his prose, showing an

  13. L’ombre du Condor

    Directory of Open Access Journals (Sweden)

    Franck Gaudichaud

    2003-09-01

    Full Text Available Si l’on observe aujourd’hui, avec un regard d’historien, le Cône Sud à la fin des années soixante soixante-dix et que par, la suite, on tourne une page de quelques années, pour fixer la même partie du monde, le constat est sans appel : le sous-continent latino-américain est passé, en général, d’une phase de forte mobilisation et politisation sociale, montée en puissance de partis et organisations révolutionnaires, surgissements de gouvernements populistes de gauche ou progressistes – appelant à une rupture avec l’impérialisme –, à un reflux généralisé du mouvement ouvrier, une ère de violence politique étatisée, la destruction massive de tous les espaces d’expression et de participation démocratiques, l’écrasement physique et idéologique sans relâche des militants et mouvements révolutionnaires, la mise en place de modèles économiques capitalistes dirigistes puis/ou néolibéraux. Que s’est-il passé ? Quel fil conducteur a été rompu et par quels moyens l’Amérique Latine a été conduite à ce reflux des luttes sociales ? Pour répondre à cette question, nous nous contenterons dans cet article de focaliser notre objectif sur un aspect spécifique et essentiel de ce retournement de tendance que vit alors l���Amérique Latine : celui de la mise en place d’un terrorisme d’Etat contre-révolutionnaire transnational, appuyé par l’impérialisme américain, nommé « l’Opération Condor ». Dans cet article, il s’agit de montrer le fonctionnement d’un terrorisme hégémonique dont l’impact sur les sociétés latino-américaines est considérable en termes de destruction du mouvement social, des activités culturelles et de l’ensemble de la vie sociale et politique qui restent marquées, encore aujourd’hui à des degrés divers, par la violence, la peur et l’atomisation structurelle.Si miramos hoy en día, desde una perspectiva histórica, el Cono Sur hacia finales de

  14. Identification and molecular characterization of two novel mutations in COL1A2 in two Chinese families with osteogenesis imperfecta

    Institute of Scientific and Technical Information of China (English)

    Zhenping Xu; Yulei Li; Xiangyang Zhang; Fanming Zeng; Mingxiong Yuan; Mugen Liu; Qing Kenneth Wang; Jing Yu Liu

    2011-01-01

    Osteogenesis imperfecta (OI, also known as brittle bone disease) is caused mostly by mutations in two type Ⅰ collagen genes, COL1A1 and COL1A2 encoding the pro-α1 (Ⅰ) and pro-α2 (Ⅰ) chains of type Ⅰ collagen, respectively. Two Chinese families with autosomal dominant OI were identified and characterized. Linkage analysis revealed linkage of both families to COL1A2 on chromosome 7q21.3-q22.1. Mutational analysis was carried out using direct DNA sequence analysis. Two novel missense mutations, c.3350A>G and c.3305G>C, were identified in exon 49 of COL1A2 in the two families, respectively. The c.3305G>C mutation resulted in substitution of a glycine residue (G) by an alanine residue (A) at codon 1102 (p.G1102A), which was found to be mutated into serine (S), argine (R), aspartic acid (D), or valine (V) in other families. The c.3350A>G variant may be a de novo mutation resulting in p.Y1117C. Both mutations co-segregated with OI in respective families, and were not found in 100 normal controls. The G1102 and Y1117 residues were evolutionarily highly conserved from zebrafish to humans. Mutational analysis did not identify any mutation in the COX-2 gene (a modifier gene of OI). This study identifies two novel mutations p.G1102A and p.Y1117C that cause OI, significantly expands the spectrum of COL1A2 mutations causing OI, and has a significant implication in prenatal diagnosis of OI.

  15. Novel PAX9 and COL1A2 missense mutations causing tooth agenesis and OI/DGI without skeletal abnormalities.

    Directory of Open Access Journals (Sweden)

    Shih-Kai Wang

    Full Text Available Inherited dentin defects are classified into three types of dentinogenesis imperfecta (DGI and two types of dentin dysplasia (DD. The genetic etiology of DD-I is unknown. Defects in dentin sialophosphoprotein (DSPP cause DD type II and DGI types II and III. DGI type I is the oral manifestation of osteogenesis imperfecta (OI, a systemic disease typically caused by defects in COL1A1 or COL1A2. Mutations in MSX1, PAX9, AXIN2, EDA and WNT10A can cause non-syndromic familial tooth agenesis. In this study a simplex pattern of clinical dentinogenesis imperfecta juxtaposed with a dominant pattern of hypodontia (mild tooth agenesis was evaluated, and available family members were recruited. Mutational analyses of the candidate genes for DGI and hypodontia were performed and the results validated. A spontaneous novel mutation in COL1A2 (c.1171G>A; p.Gly391Ser causing only dentin defects and a novel mutation in PAX9 (c.43T>A; p.Phe15Ile causing hypodontia were identified and correlated with the phenotypic presentations in the family. Bone radiographs of the proband's dominant leg and foot were within normal limits. We conclude that when no DSPP mutation is identified in clinically determined isolated DGI cases, COL1A1 and COL1A2 should be considered as candidate genes. PAX9 mutation p.Phe15Ile within the N-terminal β-hairpin structure of the PAX9 paired domain causes tooth agenesis.

  16. Control del colapso del colágeno: desproteinización Control of the collagen network collapse: collagen remoral

    Directory of Open Access Journals (Sweden)

    E Osario Ruiz

    2004-06-01

    Full Text Available La penetración de la resina y su reacción con los componentes de los tejidos dentales desmineralizados es el mecanismo micromecánico con el cual los materiales restauradores de resina se unen al diente. Los monómeros penetran en la dentina acondicionada y se concentran en la región superficial contribuyendo a la unión. El refuerzo de esta zona con resina se conoce como hibridación, e implica la impregnación de la red de colágeno y el encapsulamiento de los cristales de hidroxiapatita. Han surgido dudas en cuanto a la contribución de la red de colágeno en el ensamblaje de la unión y en la fuerza interfacial, ya que el aumento del grosor de la red de colágeno no aumenta dicha fuerza y la remoción del colágeno con hipoclorito sódico al 5% en solución acuosa da valores similares de fuerza a los que se obtienen cuando la red de colágeno está presenteResin penetration into and its reaction with the components of conditioned, mineralised dental tissues is known to be the fundamental micromechanical mechanism by which restorative materials can be successfully bonded to teeth. Monomers penetrated into conditioned dentin and concentrated in a superficial region of resin-reinforced dentin contributing significantly to the bond. The reinforcement, known as hybridization, involved impregnation, co-mingling and envelopment of collagen bundles and encapsulation of hydroxyapatite crystallites. A doubt raised concerning the quantitative contribution which the collagen network made to interfacial bond strength. lncreasing the thickness of the collagen network did not influence the assembly strength, and removal of the collagen with 5% sodium hypochlorite provided similar values to those recorded when the network was present.

  17. Affinité et organisation écologique du peuplement coléoptèrologique des écosystèmes dunaires mobiles au niveau de la frange septentrionale et de la région oriental du Maroc

    Directory of Open Access Journals (Sweden)

    Khalid BOURAADA

    2015-01-01

    Full Text Available The present work aims for a better understanding of the ecological organization of entomological groups of beetles found in mobile dunes of Eastern Morocco and in the northern fringe of the Sahara. This study is carried out over a route of 4000 km from the Guercif dunes (north to the large dunes of Merzouga (Sahara, passing by dunes of the following stations: Northeast Missour ; Midelt, Aïn Beni Methar, Tendrara, Rich, Boulman, Bouârfa, Boudnib and Erfoud. From analysis of affinity, three major faunal groups were identified. The first group is formed by the northern stations (Guercif, Northeast Missour, Midelt and Aïn Beni Methar housing faunal species of the Mediterranean dunes. The second group consists of Tendrara, Bouârfa, Rich and Bouânane stations, having a high level of fauna similarity due in part to their proximity. The last group was formed by the station of Merzouga, Erfoud, Boudnib and Figuig which feature a frankly Saharan population. Taxa collected in these Saharan stations were collected along a north-south geographical gradient whose northern end is formed by the characteristic species of the Mediterranean area while the southern end is made of the wildlife species characteristic of the Saharan fringe.

  18. Clinical and anatomical guidelines in pelvic cancer contouring for radiotherapy treatment planning; Definition de regles simples anatomocliniques dans la determination du volume cible des tumeurs pelviennes pour le planning radiotherapeutique

    Energy Technology Data Exchange (ETDEWEB)

    Portaluri, M.; Bambace, S.; Giuliano, G.; Pili, G.; Didonna, V. [General Hospital Di Summa-Perrino, Dept. of Radiation Oncology, Medical Physics, Brindisi (Italy); Perez, C.; Angone, G.; Alloro, E. [General Hospital Di Summa-Perrino, Dept. of Radiology, Medical Physics, Brindisi (Italy); Scialpi, M. [General Hospital SS. Annunziata, Dept. of Radiology, Taranto (Italy)

    2004-08-01

    Background and purpose. Many observations on potential inadequate coverage of tumour volume at risk in advanced cervical cancer (CC) when conventional radiation fields are used, have further substantiated by investigators using MRI, CT or lymph-angiographic imaging. This work tries to obtain three dimensional margins by observing enlarged nodes in CT scans in order to improve pelvic nodal chains clinical target volumes (CTVs) drawing, and by looking for corroborative evidence in the literature for a better delineation of tumour CTV. Method. Eleven consecutive patients (seven males, four females, mean age 62 years, range 43 8) with CT diagnosis of nodal involvement caused by pathologically proved carcinoma of the cervix (n = 2), carcinoma of the rectum (n = 2), carcinoma of the prostate (n = 2), non-Hodgkin lymphoma (n 2), Hodgkin lymphoma (n = 1), carcinoma of the penis (n = 1) and carcinoma of the corpus uteri (n = 1) were retrospectively reviewed. Sixty CT scans with 67 enlarged pelvic nodes were reviewed in order to record the more proximal structures (muscle, bone, vessels, cutis or sub-cutis and other organs) to each enlarged node or group of nodes according to the four surfaces (anterior, lateral, posterior and medial) in a clockwise direction. Results. summary of the observations of each nodal chain and the number of occurrences of every marginal structure on axial CT slices is presented. Finally, simple guidelines are proposed. Conclusions. Tumour CTV should be based on individual tumour anatomy mainly for lateral beams as it results from sagittal T2 weighted MRI images. Boundaries of pelvic nodes CTVs can be derived from observations of enlarged lymph nodes in CT scans. (author)

  19. Chemical chaperone treatment reduces intracellular accumulation of mutant collagen IV and ameliorates the cellular phenotype of a COL4A2 mutation that causes haemorrhagic stroke.

    Science.gov (United States)

    Murray, Lydia S; Lu, Yinhui; Taggart, Aislynn; Van Regemorter, Nicole; Vilain, Catheline; Abramowicz, Marc; Kadler, Karl E; Van Agtmael, Tom

    2014-01-15

    Haemorrhagic stroke accounts for ∼20% of stroke cases and porencephaly is a clinical consequence of perinatal cerebral haemorrhaging. Here, we report the identification of a novel dominant G702D mutation in the collagen domain of COL4A2 (collagen IV alpha chain 2) in a family displaying porencephaly with reduced penetrance. COL4A2 is the obligatory protein partner of COL4A1 but in contrast to most COL4A1 mutations, the COL4A2 mutation does not lead to eye or kidney disease. Analysis of dermal biopsies from a patient and his unaffected father, who also carries the mutation, revealed that both display basement membrane (BM) defects. Intriguingly, defective collagen IV incorporation into the dermal BM was observed in the patient only and was associated with endoplasmic reticulum (ER) retention of COL4A2 in primary dermal fibroblasts. This intracellular accumulation led to ER stress, unfolded protein response activation, reduced cell proliferation and increased apoptosis. Interestingly, the absence of ER retention of COL4A2 and ER stress in cells from the unaffected father indicate that accumulation and/or clearance of mutant COL4A2 from the ER may be a critical modifier for disease development. Our analysis also revealed that mutant collagen IV is degraded via the proteasome. Importantly, treatment of patient cells with a chemical chaperone decreased intracellular COL4A2 levels, ER stress and apoptosis, demonstrating that reducing intracellular collagen accumulation can ameliorate the cellular phenotype of COL4A2 mutations. Importantly, these data highlight that manipulation of chaperone levels, intracellular collagen accumulation and ER stress are potential therapeutic options for collagen IV diseases including haemorrhagic stroke.

  20. Molecular evolution and phylogenetic analysis of eight COL superfamily genes in group I related to photoperiodic regulation of flowering time in wild and domesticated cotton (Gossypium) species.

    Science.gov (United States)

    Zhang, Rui; Ding, Jian; Liu, Chunxiao; Cai, Caiping; Zhou, Baoliang; Zhang, Tianzhen; Guo, Wangzhen

    2015-01-01

    Flowering time is an important ecological trait that determines the transition from vegetative to reproductive growth. Flowering time in cotton is controlled by short-day photoperiods, with strict photoperiod sensitivity. As the CO-FT (CONSTANS-FLOWER LOCUS T) module regulates photoperiodic flowering in several plants, we selected eight CONSTANS genes (COL) in group I to detect their expression patterns in long-day and short-day conditions. Further, we individually cloned and sequenced their homologs from 25 different cotton accessions and one outgroup. Finally, we studied their structures, phylogenetic relationship, and molecular evolution in both coding region and three characteristic domains. All the eight COLs in group I show diurnal expression. In the orthologous and homeologous loci, each gene structure in different cotton species is highly conserved, while length variation has occurred due to insertions/deletions in intron and/or exon regions. Six genes, COL2 to COL5, COL7 and COL8, exhibit higher nucleotide diversity in the D-subgenome than in the A-subgenome. The Ks values of 98.37% in all allotetraploid cotton species examined were higher in the A-D and At-Dt comparison than in the A-At and D-Dt comparisons, and the Pearson's correlation coefficient (r) of Ks between A vs. D and At vs. Dt also showed positive, high correlations, with a correlation coefficient of at least 0.797. The nucleotide polymorphism in wild species is significantly higher compared to G. hirsutum and G. barbadense, indicating a genetic bottleneck associated with the domesticated cotton species. Three characteristic domains in eight COLs exhibit different evolutionary rates, with the CCT domain highly conserved, while the B-box and Var domain much more variable in allotetraploid species. Taken together, COL1, COL2 and COL8 endured greater selective pressures during the domestication process. The study improves our understanding of the domestication-related genes/traits during cotton

  1. Utilisation du langage signal pour l'etude d'algorithmes de traitement du Signal electrocardiographique

    OpenAIRE

    Médigue, Claire; Clairambault, Jean; Kauffmann, François; Sorine, Michel; Cursi-Dascalova, Lilia

    1992-01-01

    Projet SOSSO; Pour etudier la maturation du Systeme Nerveux Autonome (SNA) chez les nouveaux nes, on analyse la Variabilite du Rythme Cardiaque (VRC), en relation avec d'autres informations biologiques telles que les changements de stades de sommeil. La VRC est calculee par la Transformee de Fourier a Court Terme dans trois bandes de frequence : la haute frequence, refletant le controle para sympathique seul et liee au cycle respiratoire ; les moyenne et basse frequences, refletant a la fois ...

  2. OGM : vers une définition commune du contenu du problème ?

    Directory of Open Access Journals (Sweden)

    Icart Jean-Claude

    2001-07-01

    Full Text Available Les divergences croissantes depuis des années entre experts, responsables économiques, et acteurs sociaux et « citoyens » au sujet des OGM, ont pu être analysées à juste titre, comme exprimant la difficulté de dégager une « définition commune du contenu du problème »1.

  3. 中国汉族病理性近视人群的COL9A2基因突变分析%Analysis of COL9A2 gene mutations in a Chinese Han population with pathological myopia

    Institute of Scientific and Technical Information of China (English)

    陈蓉; 龚波; 李倩; 曾光群; 郝(芳); 李宁; 石毅; 张丁丁

    2014-01-01

    目的 对COL9A2基因进行突变分析,探讨中国汉族病理性近视的分子发病机制.方法 选取400例病理性近视患者和400名正常对照,对其中200例患者和200名对照的COL9A2基因全部外显子进行Sanger测序;对检测到的变异位点,应用SNaPshot方法在另外200例患者和200名对照中进行基因分型.结果 通过Sanger测序,在200例病理性近视患者中未检出已报道的D281fs移码突变,但1例患者检测出COL9A2基因第2外显子c.143G>C杂合错义突变,另外1例患者检测出COL9A2基因第17外显子c.884G>A杂合错义突变,在正常对照中未检测到c.143G>C和c.884G>A突变.同时在外显子编码区检测到COL9A2基因rs2228564 SNP位点,其等位基因频率在患者组与正常对照组间的差异无统计学意义(P>0.05).应用SNaPshot方法对另外的200例患者和200名正常对照的这些位点进行基因分型验证,1例患者检测到COL9A2基因第2外显子c.143G>C突变,2例患者检测到COL9A2基因第17外显子c.884G>A突变,rs2228564 SNP位点的等位基因频率在两组间的差异无统计学意义(P>0.05).结论 中国汉族病理性近视与COL9A2基因D281fs移码突变可能无关,COL9A2基因第2外显子c.143G>C突变和第17外显子c.884G>A突变可能与病理性近视的发病有关.%Objective To analyze the mutation of COL9A2 gene and investigate the molecular pathogenesis of pathological myopia in a Han Chinese population.Methods Mutation in the coding region of the COL9A2 gene was screened by Sanger sequencing in 200 subjects with pathological myopia and 200 normal controls.The detected variants were genotyped by SNaPshot method in another 200 myopic cases and 200 normal controls.Results Sanger sequencing has failed to detect the reported D281fs frameshift mutation in the 200 cases.A novel variant,c.143G>C heterozygous missense mutation in exon 2,was identified in a myopic subject,and another novel variant,c.884G

  4. Clinical Application of the Du Channel

    Institute of Scientific and Technical Information of China (English)

    刘淑娅; 王世杰

    2003-01-01

    @@ The authors have, in recent years, obtained quite good therapeutic results in their acupuncture treatment of mental diseases, heat syndromes and cerebrovascular diseases through treating mainly the Du Channel with the methods of resuscitating and regulating the mind, and clearing and activating the channels and collaterals. The illustrative cases are reported below.

  5. ESTIMATION DU MODELE LINEAIRE GENERALISE ET APPLICATION

    Directory of Open Access Journals (Sweden)

    Malika CHIKHI

    2012-06-01

    Full Text Available Cet article présente  le modèle linéaire généralisé englobant les  techniques de modélisation telles que la régression linéaire, la régression logistique, la régression  log linéaire et la régression  de Poisson . On Commence par la présentation des modèles  des lois exponentielles pour ensuite estimer les paramètres du modèle par la méthode du maximum de vraisemblance. Par la suite on teste les coefficients du modèle pour voir leurs significations et leurs intervalles de confiances, en utilisant le test de Wald qui porte sur la signification  de la vraie valeur du paramètre  basé sur l'estimation de l'échantillon.

  6. Néotoponymie contestée à Potchefstroom / Tlokwe (Province du Nord-Ouest, Afrique du Sud

    Directory of Open Access Journals (Sweden)

    Myriam Houssay-Holzschuch

    2010-09-01

    Full Text Available Illustration 1 - Extrait du site de la municipalité de Potchefstroom / TlokweSource : http://www.potch.co.za/newsarchive/streetnames.htmlIllustration 2- Potchefstroom (Afrique du SudAuteur : Béatrice Obry-Guyot, début décembre 2007.Illustration 3 - Extrait du plan Google Map de PotchefstroomSource : Google Map.La question toponymique en Afrique du Sud sur la longue durée porte sur deux thèmes essentiels porteurs de controverses : le plurilinguisme et le marquage symbolique et mémoriel du te...

  7. Sébastien Broca, Utopie du logiciel libre. Du bricolage informatique à la réinvention sociale

    OpenAIRE

    Goëta, Samuel

    2014-01-01

    Traitant des pratiques et des conceptions du logiciel libre mais aussi, plus largement, du mouvement pour l’ouverture des savoirs, Utopie du logiciel libre ambitionne, premièrement, de nourrir une réflexion critique sur l’ethos du libre au sens d’un « ensemble de valeurs qui constituent un cadre pour l’action et s’actualisent dans des pratiques » (p. 24) et, deuxièmement, d’élaborer une sociologie critique du libre. Reprenant la notion d’utopie concrète chez Ernst Bloch (Le principe Espérance...

  8. ColE1-plasmid production in Escherichia coli: Mathematical Simulation and Experimental Validation

    Directory of Open Access Journals (Sweden)

    Inga eFreudenau

    2015-09-01

    Full Text Available Plasmids have become very important as pharmaceutical gene vectors in the fields of gene therapy and genetic vaccination in the last years. In this study, we present a dynamic model to simulate the ColE1-like plasmid replication control, once for a DH5α-strain carrying a low copy plasmid (DH5α-pSUP 201-3 and once for a DH5α-strain carrying a high copy plasmid (DH5α-pCMV-lacZ by using ordinary differential equations (ODE and the MATLAB software. The model includes the plasmid replication control by two regulatory RNA molecules (RNAI and RNAII as well as the replication control by uncharged tRNA molecules. To validate the model, experimental data like RNAI- and RNAII concentration, plasmid copy number (PCN, and growth rate for three different time points in the exponential phase were determined. Depending on the sampled time point, the measured RNAI and RNAII concentrations for DH5α-pSUP 201-3 reside between 6 ±0.7 to 34 ±7 RNAI molecules per cell and 0.44 ±0.1 to 3 ±0.9 RNAII molecules per cell. The determined plasmid copy numbers (PCN averaged between 46 ±26 to 48 ±30 plasmids per cell. The experimentally determined data for DH5α-pCMV-lacZ reside between 345 ±203 to 1086 ±298 RNAI molecules per cell and 22 ±2 to 75 ±10 RNAII molecules per cell with an averaged PCN of 1514 ±1301 to 5806 ±4828 depending on the measured time point. As the model was shown to be consistent with the experimentally determined data, measured at three different time points within the growth of the same strain, we performed predictive simulations concerning the effect of uncharged tRNA molecules on the ColE1-like plasmid replication control. The hypothesis is that these tRNA molecules would have an enhancing effect on the plasmid production. The in silico analysis predicts that uncharged tRNA molecules would indeed increase the pDNA production.

  9. ColE1-Plasmid Production in Escherichia coli: Mathematical Simulation and Experimental Validation

    Science.gov (United States)

    Freudenau, Inga; Lutter, Petra; Baier, Ruth; Schleef, Martin; Bednarz, Hanna; Lara, Alvaro R.; Niehaus, Karsten

    2015-01-01

    Plasmids have become very important as pharmaceutical gene vectors in the fields of gene therapy and genetic vaccination in the past years. In this study, we present a dynamic model to simulate the ColE1-like plasmid replication control, once for a DH5α-strain carrying a low copy plasmid (DH5α-pSUP 201-3) and once for a DH5α-strain carrying a high copy plasmid (DH5α-pCMV-lacZ) by using ordinary differential equations and the MATLAB software. The model includes the plasmid replication control by two regulatory RNA molecules (RNAI and RNAII) as well as the replication control by uncharged tRNA molecules. To validate the model, experimental data like RNAI- and RNAII concentration, plasmid copy number (PCN), and growth rate for three different time points in the exponential phase were determined. Depending on the sampled time point, the measured RNAI- and RNAII concentrations for DH5α-pSUP 201-3 reside between 6 ± 0.7 and 34 ± 7 RNAI molecules per cell and 0.44 ± 0.1 and 3 ± 0.9 RNAII molecules per cell. The determined PCNs averaged between 46 ± 26 and 48 ± 30 plasmids per cell. The experimentally determined data for DH5α-pCMV-lacZ reside between 345 ± 203 and 1086 ± 298 RNAI molecules per cell and 22 ± 2 and 75 ± 10 RNAII molecules per cell with an averaged PCN of 1514 ± 1301 and 5806 ± 4828 depending on the measured time point. As the model was shown to be consistent with the experimentally determined data, measured at three different time points within the growth of the same strain, we performed predictive simulations concerning the effect of uncharged tRNA molecules on the ColE1-like plasmid replication control. The hypothesis is that these tRNA molecules would have an enhancing effect on the plasmid production. The in silico analysis predicts that uncharged tRNA molecules would indeed increase the plasmid DNA production. PMID:26389114

  10. Esquisse du parlementarisme et du monopartisme en Afrique: le cas du Togo

    Directory of Open Access Journals (Sweden)

    E. A. B. van Rouveroy van Nieuwaal

    1989-01-01

    Full Text Available Witnessed the last hundred years profound political and constitutional changes. In this respect there are many differences between African States depending on which kind of colonial overlord has been the ruling power. The African State was a juridical entity in international law, but was it also, at the time of independence, an empirical entity in national fact?In almost all cases the empirical reality as a functioning government was still primarily the presence of European bureaucrats who has embodied the colonial state. Independence therefore opened a gap between the international legitimacy and the internal marginality of many emergent African State. The gap often presented a real political dilemma to the new African rulers: they usually could retain European officials only by compromising their national independence and could dispense with them only at the risk of undermining governmental performance. After adoption of European constitutional law and with, initially, a high degree of similarity between most constitutions in French-speaking Africa, nowadays these constitutions differ widely, contrary to the Commonwealth Africa that has experienced fewer innovations and constitutional breakdowns.Parliamentary systems of government gave way to One Party Systems, introduced in many cases by the national army, as the best equiped, trained, paid and organised power in the country. This has been the political and constitutional development in Togo as well. Since 1969, it has a One Party System, the Rassemblement du Peuple Togolais, founded by the President of the Togolese Republic, Gen. Gnassingbé Eyadéma. But, as everywhere else, doubts are growing about the effectiveness of the One Party System. Are the Togolese perspectives such that Eyadéma is willing to have an open mind and to be all ears for the critics to his own creation? Another intriguing question is to know in which way the Africantraditional authorities (" chieftancy", as the core

  11. Therapy choice for rectum neoplasm; Le choix des therapeutiques du cancer du rectum

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    1995-06-01

    The tumour extension degree is given essentially by the rectal palpation and endo-rectal echography. The recent surgical technologies allow more and more often to keep the anal sphincter. The surgical ablation quality is a remote factor prognostic. The treatment decisions must be taken by trained pluridisciplinar teams.

  12. Osteoporosis and Related Genes: VDR, ESR And COL1A1

    Directory of Open Access Journals (Sweden)

    Sabriye Kocaturk Sel

    2011-08-01

    Full Text Available Osteoporosis is now considered as one of the major and growing health care problems around the world. Osteoporosis is the most prevalent metabolic bone disease among developed countries and it is defined as a systemic skeletal disease characterized by low bone mass and microarchitectural deterioration of bone tissue with a consequent increase in bone fragility. Bone is a highly metabolically active tissue in which the processes of osteoblastic bone formation and osteoclastic bone resorption are continuous throughout life. Coupling of osteoblast and osteoclast action ensures that a normal bone structure is maintained. A loss of bone homeostasis may result in a decrease in bone mass leading to osteoporosis or in a defect in the mineralization of bone. Numerous genetic, hormonal, nutritional and life-style factors contribute to the acquisition and maintenance of bone mass. Among them, genetic variations explain as much as 50-80% of the variance for bone mineral density (BMD in the population. Many genes that could be related to osteoporosis have been studied and of them all Vitamin D receptor (VDR, estrogen receptor alpha (ESRα and collagen 1 alpha 1 chain (COL1A1 genes have been the most focused on. [Archives Medical Review Journal 2011; 20(4.000: 246-269

  13. The X-ray properties of the magnetic Cataclysmic Variable UU Col

    CERN Document Server

    De Martino, D; Mukai, K; Bonnet-Bidaud, J M; Burwitz, V; Gänsicke, B T; Haberl, F; Mouchet, M

    2006-01-01

    XMM-Newton observations aimed at determining for the first time the broad-band X-ray properties of the faint high galactic latitude Intermediate Polar UU Col are presented. We performed X-ray timing analysis in different energy ranges of the EPIC cameras which reveals the dominance of the 863s white dwarf rotational period. The spin pulse is strongly energy dependent. Weak variabilities at the beat 935s and at the 3.5hr orbital periods are also observed, but the orbital modulation is detected only below 0.5keV. Simultaneous UV and optical photometry shows that the spin pulse is anti-phased with respect to the hard X-rays. Analysis of the EPIC and RGS spectra reveals the complexity of the X-ray emission, being composed of a soft 50eV black--body component and two optically thin emission components at 0.2keV and 11keV strongly absorbed by dense material with an equivalent hydrogen column density of 10**(23)cm**(-2) partially (50%) covering the X-ray source. The complex X-ray and UV/optical temporal behaviour in...

  14. Cartografia e deficiência visual: experiências no Colégio Pedro II

    Directory of Open Access Journals (Sweden)

    Rafael Medeiros de Andrade

    2014-01-01

    Full Text Available O processo de construção de uma escola inclusiva perpassa um conjunto de esforços cognitivos e técnicos por parte da comunidade escolar que são necessários para a adequada educação e desenvolvimento do aluno. No que diz respeito ao ensino de Geografia para alunos deficientes visuais, fazem parte deste conjunto de medidas o domínio do sistema Braille, a confecção de materiais e mapas táteis, a gravação de textos em áudio e, entre outras, a realização de trabalhos de campo. O presente relato de experiência refere-se à um projeto de dedicação exclusiva, implementado no Colégio Pedro II de 2008 a 2010, cujo objetivo foi aprimorar o ensino de Geografia para os alunos deficientes visuais. Conclui-se que a adoção dessas medidas representa o início de um longo trabalho de toda comunidade escolar que não pode ser resumido à existência de alguns materiais táteis. Deve, por outro lado, envolver um processo de construção e adequação instrumental, acompanhado da capacitação profissional e da sensibilização da comunidade escolar.

  15. Xarxa interclase: un projecte col·laboratiu mitjançant tecnologies digitals

    Directory of Open Access Journals (Sweden)

    Françoise Olmo Cazevieille

    2015-12-01

    Full Text Available Focalitzem aquest treball en l'aportació de les tecnologies de la informació i la comunicació (TIC a la pràctica i el desenvolupament d'una llengua estrangera i en els avantatges que ofereixen per a la creació d'activitats basades en la col·laboració i la interacció. Sense aquestes, tant els treballs en equip amb nadius com la cerca d'informació sobre temes especialitzats en la llengua estudiada serien complexos de dur a terme. En aquest article presentem el procediment seguit en un projecte didàctic, Xarxa interclasse, entre dues assignatures basades en l'aprenentatge de llengües estrangeres per a fins específics en un mateix campus universitari.?Es pretén, d'una banda, acostar l'educació superior a les exigències de la societat actual i, d'altra banda, aconseguir una docència motivadora i una participació activa dels discents on la tecnologia s'integra com a element pedagògic.

  16. Two novel distinct COL1A2 mutations highlight the complexity of genotype-phenotype correlations in osteogenesis imperfecta and related connective tissue disorders.

    Science.gov (United States)

    Reuter, Miriam S; Schwabe, Georg C; Ehlers, Christian; Marschall, Christoph; Reis, André; Thiel, Christian; Graul-Neumann, Luitgard

    2013-12-01

    Osteogenesis imperfecta is a heritable connective tissue disorder characterized by variable symptoms including predisposition to fractures. Despite the identification of numerous mutations, a reliable genotype-phenotype correlation has remained notoriously difficult. We now describe two patients with osteogenesis imperfecta and novel, so far undescribed mutations in the COL1A2 gene, further highlighting this complexity. A 3-year-old patient presented with features reminiscent of a connective tissue disorder, with joint hypermobility, Wormian bones, streaky lucencies in the long bones and relative macrocephaly. The patient carried a heterozygous c.1316G > A (p.Gly439Asp) mutation in the COL1A2 gene located in a triple-helix region, in which glycine substitutions have been assumed to cause perinatal lethal OI (Sillence type II). A second family with type I osteogenesis imperfecta carried a heterozygous nonsense mutation c.4060C > T (p.Gln1354X) within the last exon of COL1A2. Whereas other heterozygous nonsense mutations in COL1A2 do not lead to a phenotype, in this case the mRNA is presumed to escape nonsense-mediated decay. Therefore the predicted COL1A2 propeptide lacks the last 13 C-terminal amino acids, suggesting that the OI phenotype results from decelerated assembly and overmodification of the collagen triple helix. The presented COL1A2 mutations exemplify the complexity of COL1A2 genotype-phenotype correlation in genetic counselling in OI.

  17. PTH stimulated growth and decreased Col-X deposition are phosphotidylinositol-3,4,5 triphosphate kinase and mitogen activating protein kinase dependent in avian sterna.

    Science.gov (United States)

    Harrington, Erik Kern; Coon, David J; Kern, Matthew F; Svoboda, Kathy K H

    2010-02-01

    Type X collagen (Col-X) deposition is a marker of terminal differentiation during chondrogenesis, in addition to appositional growth and apoptosis. The parathyroid hormone/parathyroid hormone related peptide (PTH/PTHrP) receptor, or PPR, is a G-Protein coupled receptor (GPCR), which activates several downstream pathways, moderating chondrocyte differentiation, including suppression of Col-X deposition. An Avian sterna model was used to analyze the PPR GPCR downstream kinase role in growth rate and extracellular matrix (ECM) including Col-II, IX, and X. Phosphatidylinositol kinase (PI3K), mitogen activating protein kinase (MAPK) and protein kinase A (PKA) were inhibited with specific established inhibitors LY294002, PD98059, and H89, respectively to test the hypothesis that they could reverse/inhibit the PTH/PTHrP pathway. Excised E14 chick sterna were PTH treated with or without an inhibitor and compared to controls. Sternal length was measured every 24 hr. Cultured sterna were immuno-stained using specific antibodies for Col-II, IX, or X and examined via confocal microscopy. Increased growth in PTH-treated sterna was MAPK, PI3K, and PKA dose dependent, suggesting growth was regulated through multiple pathways. Col-X deposition was rescued in PTH-treated sterna in the presence of PI3K or MAPK inhibitors, but not with the PKA inhibitor. All three inhibitors moderately disrupted Col-II and Col-IX deposition. These results suggest that PTH can activate multiple pathways during chondrocyte differentiation.

  18. Repair of large osteochondral defects in rabbits using porous hydroxyapatite/collagen (HAp/Col) and fibroblast growth factor-2 (FGF-2).

    Science.gov (United States)

    Maehara, Hidetsugu; Sotome, Shinichi; Yoshii, Toshitaka; Torigoe, Ichiro; Kawasaki, Yuichi; Sugata, Yumi; Yuasa, Masato; Hirano, Masahiro; Mochizuki, Naomi; Kikuchi, Masanori; Shinomiya, Kenichi; Okawa, Atsushi

    2010-05-01

    Articular cartilage has a limited capacity for self-renewal. This article reports the development of a porous hydroxyapatite/collagen (HAp/Col) scaffold as a bone void filler and a vehicle for drug administration. The scaffold consists of HAp nanocrystals and type I atelocollagen. The purpose of this study was to investigate the efficacy of porous HAp/Col impregnated with FGF-2 to repair large osteochondral defects in a rabbit model. Ninety-six cylindrical osteochondral defects 5 mm in diameter and 5 mm in depth were created in the femoral trochlear groove of the right knee. Animals were assigned to one of four treatment groups: porous HAp/Col impregnated with 50 microl of FGF-2 at a concentration of 10 or 100 microg/ml (FGF10 or FGF100 group); porous HAp/Col with 50 microl of PBS (HAp/Col group); and no implantation (defect group). The defect areas were examined grossly and histologically. Subchondral bone regeneration was quantified 3, 6, 12, and 24 weeks after surgery. Abundant bone formation was observed in the HAp/Col implanted groups as compared to the defect group. The FGF10 group displayed not only the most abundant bone regeneration but also the most satisfactory cartilage regeneration, with cartilage presenting a hyaline-like appearance. These findings suggest that porous HAp/Col with FGF-2 augments the cartilage repair process.

  19. Trans-activation of Col1a1 Promoter by Osterix During Osteocyte Differentiation%转录因子Osterix对成骨细胞Col1a1基因的反式激活作用

    Institute of Scientific and Technical Information of China (English)

    潘秋辉; 马纪; 于永春; 孙奋勇

    2007-01-01

    Osterix(Osx)是一种具有锌指结构的转录因子,对骨形成十分重要.但到目前为止,直接接受Osterix调控的靶基因尚不清楚.用骨形态发生蛋白2(bone morphogenetic protein 2,BMP2)诱导原代培养小鼠成骨细胞的骨分化,定量RT-PCR检测Ⅰ型胶原蛋白(collagen Ⅰ a 1,Col1a1)与Osx的转录水平.结果发现,二者的转录时相具有相同的变化模式.为了确定二者之间的关系,采用腺病毒表达系统在原代成骨细胞中过表达Osx.数据表明,Osx能够明显上调Col1a1的转录水平.用EMSA(electromobility shift assay)检测这些过表达Osx基因的成骨细胞核抽提物,迁移条带的出现表明,Osx能够直接与Col1a1的启动子相结合.结果提示,在原代培养的成骨细胞中,Osterix通过直接与启动子相结合,调控Col1a1基因的转录.

  20. The pursuit of the genome instability by comet assay (single cell gel electrophoresis) in patients with cancer of the cavum in western Algerian; La recherche de l'instabilite genomique par le test des cometes (single cell gel electrophoresis) chez les malades atteints d'un cancer du cavum dans l'Ouest algerien

    Energy Technology Data Exchange (ETDEWEB)

    Boukerche, A.; Dali-Youcef, A.F. [Service de Radiotherapie, Oran (Algeria); Bouali-Youcef, Y. [Laboratoire d' Immunologie, Oran (Algeria); Mehadji, M. [Service d' ORL, Oran (Algeria); Chenal, C. [Rennes-1 Univ., UMR CNRS 6853, 35 (France)

    2007-11-15

    The analysis of results has shown a constitutional genome instability among the patients with a cavum cancer with a defect in DNA repair where some exogenous factors ( Epstein-Barr virus, EBV) seem play an important part. (N.C.)

  1. Col2CreERT2, a mouse model for a chondrocyte-specific and inducible gene deletion

    Directory of Open Access Journals (Sweden)

    M Chen

    2014-10-01

    Full Text Available In 2007 and 2008, we published two articles reporting a tamoxifen (TM-inducible, chondrocyte-specific gene-targeting mouse model in which the expression of CreERT2 is driven by the type II collagen promoter (Col2CreERT2. The fusion protein is specifically expressed and translocated into the nucleus upon TM administration, which in turn triggers gene recombination. Since then, this animal model has become a powerful tool to study the molecular mechanism of skeletal development and degenerative cartilage diseases, including knee joint osteoarthritis (OA, temporomandibular joint (TMJ OA, and intervertebral disc (IVD degeneration. In this review article, we summarise the application of Col2CreERT2 mice and discuss the potential usage of this animal model in a broad spectrum of cartilage development and molecular pathology studies.

  2. Different NaCl-Induced Calcium Signatures in the Arabidopsis thaliana Ecotypes Col-0 and C24

    KAUST Repository

    Schmöckel, Sandra M.

    2015-02-27

    A common feature of stress signalling pathways are alterations in the concentration of cytosolic free calcium ([Ca2+]cyt), which allow the specific and rapid transmission of stress signals through a plant after exposure to a stress, such as salinity. Here, we used an aequorin based bioluminescence assay to compare the NaCl-induced changes in [Ca2+]cyt of the Arabidopsis ecotypes Col-0 and C24. We show that C24 lacks the NaCl specific component of the [Ca2+]cyt signature compared to Col-0. This phenotypic variation could be exploited as a screening methodology for the identification of yet unknown components in the early stages of the salt signalling pathway.

  3. Different NaCl-induced calcium signatures in the Arabidopsis thaliana ecotypes Col-0 and C24.

    Science.gov (United States)

    Schmöckel, Sandra M; Garcia, Alexandre F; Berger, Bettina; Tester, Mark; Webb, Alex A R; Roy, Stuart J

    2015-01-01

    A common feature of stress signalling pathways are alterations in the concentration of cytosolic free calcium ([Ca2+]cyt), which allow the specific and rapid transmission of stress signals through a plant after exposure to a stress, such as salinity. Here, we used an aequorin based bioluminescence assay to compare the NaCl-induced changes in [Ca2+]cyt of the Arabidopsis ecotypes Col-0 and C24. We show that C24 lacks the NaCl specific component of the [Ca2+]cyt signature compared to Col-0. This phenotypic variation could be exploited as a screening methodology for the identification of yet unknown components in the early stages of the salt signalling pathway.

  4. L'organització de l'activitat conjunta en l'ensenyament escolar dels esports col·lectius

    OpenAIRE

    2001-01-01

    L'estudi de les formes d'organització de l'activitat conjunta en l'ensenyament de la col·laboració tàctica en els esports col·lectius en l'àmbit escolar mostra com a punts de referència teòrica, per un costat aportacions provinents de la psicologia de la instrucció, i per un altre costat aportacions provinents de la didàctica de l' ensenyament de l' educació física i l' esport. Pel que fa al camp de la psicologia, cal situar l'estudi de l'activitat conjunta en una perspectiva constructivista ...

  5. Présentation du volume

    Directory of Open Access Journals (Sweden)

    Ana Zwitter Vitez

    2012-12-01

    Full Text Available Dans de nombreuses disciplines scientifiques, le discours parlé représente depuis quelques décennies un objet de recherche proéminent. Ce fait s’explique probablement par deux caractéristiques principales du discours parlé: la structure complexe de sa matérialité et les fonctions variées de différentes pratiques langagières. C’est pourquoi le présent numéro réunit les articles basés sur les approches actuelles du discours parlé et sur les pratiques langagières spécifiques. Le volume est introduit par deux articles interdisciplinaires: Peter Garrard et Ahmed Samrah présentent le domaine de la pathologie du langage en donnant une revue fouillée sur les approches actuelles de l’analyse linguistique lors de la détection de la maladie d’Alzheimer, tandis que Harry Hollien donne un aperçu structuré sur le domaine de la phonétique judiciaire destiné à dévoiler l’identité des locuteurs et de détecter la déception dans le discours parlé. Les analyses explorant l’acquisition du langage commencent par l’article de Katharina Zipser qui examine la progression de structures grammaticales en les mettant en comparaison avec la compétence des apprenants et continuent par l’étude de Meta Lah proposant une évaluation des documents audiovisuels proposés aux apprenants de langue étrangère. Vesna Požgaj Hadži, Damir Horga et Tatjana Balažic Bulc remettent en question la corrélation entre la compétence linguistique et la fluence linguistique auprès de locuteurs non-maternels et l'analyse de Gemma Santiago Alonso aborde l'acquisition de l'article défini dans le langage enfantin. Les articles suivants se réunissent autour du domaine de l'interprétation: Jana Zidar Forte présente une approche actuelle dans l'entrainement d'interprètes, Lea Burjan analyse les phénomènes issus de la pratique de l'interprétation juridique et Simona  Šumrada traite de la reformulation dans le discours de la traduction et de

  6. Estudi de l'eficiència energètica d'una casa de colònies

    OpenAIRE

    Masimon i Clavera, Lluís

    2009-01-01

    L’objecte del projecte és la millora de l’eficiència energètica per a la producció d’aigua calenta sanitària, calefacció, i climatització de l’aigua d’una piscina coberta, en una casa de colònies, prioritzant l’ús d’energia solar i optimitzant la gestió energètica.

  7. Extensive Analysis of GmFTL and GmCOL Expression in Northern Soybean Cultivars in Field Conditions

    OpenAIRE

    2015-01-01

    The FLOWERING LOCUS T (FT) gene is a highly conserved florigen gene among flowering plants. Soybean genome encodes six homologs of FT, which display flowering activity in Arabidopsis thaliana. However, their contributions to flowering time in different soybean cultivars, especially in field conditions, are unclear. We employed six soybean cultivars with different maturities to extensively investigate expression patterns of GmFTLs (Glycine max FT-like) and GmCOLs (Glycine max CO-like) in the f...

  8. Projecting Politics: The Grapes of Wrath Les Raisins de la colère : Une critique politique

    Directory of Open Access Journals (Sweden)

    Stephen J. Whitfield

    2008-01-01

    Full Text Available Publié en 1939 et adapté à l’écran l’année suivante, Les Raisins de la colère, roman de John Steinbeck, eut un impact immédiat et extraordinaire pour le portrait sans concession qu’il dressait d’un capitalisme américain qui semblait avoir abandonné sa progéniture la plus loyale, la plus assidue et la plus courageuse, celle qui labourait la terre en plein cœur de la république. Écrit à la toute fin de la « Décennie rouge », mais juste avant que les États-Unis entrent dans la Seconde Guerre mondiale, Les Raisins de la colère exprimaient, d’une certaine manière, la colère accumulée contre un système économique qui avait trahi le prolétariat rural. Le roman tout comme le film de John Ford (1940 furent compris par le grand public de l’époque, et bien des années plus tard, comme une protestation clairement gauchiste, voire radicale, contre une telle injustice. Mais cet essai montre combien les idées politiques de ces œuvres jumelées sont en réalité difficiles à saisir et combien il est hasardeux de définir, quel que soit l’effort rétrospectif, la critique que Steinbeck et Ford ont proposée dans leur version des Raisins de la colère, une œuvre inoubliable, déchirante mais ambiguë sur le plan politique.

  9. Projecting Politics: The Grapes of Wrath Les Raisins de la colère à l’écran

    Directory of Open Access Journals (Sweden)

    Stephen J. Whitfield

    2009-07-01

    Full Text Available Publié en 1939 et adapté à l’écran l’année suivante, Les Raisins de la colère, roman de John Steinbeck, eut un impact immédiat et extraordinaire pour le portrait sans concession qu’il dressait d’un capitalisme américain qui semblait avoir abandonné sa progéniture la plus loyale, la plus assidue et la plus courageuse, celle qui labourait la terre en plein coeur de la république. Écrit à la toute fin de la « Décennie rouge », mais juste avant que les États-Unis entrent dans la Seconde Guerre mondiale, Les Raisins de la colère exprimaient, d’une certaine manière, la colère accumulée contre un système économique qui avait trahi le prolétariat rural. Le roman, tout comme le film de John Ford (1940, furent compris par le grand public de l’époque, et bien des années plus tard, comme une protestation clairement gauchiste, voire radicale, contre une telle injustice. Mais cet essai montre combien les idées politiques de ces œuvres jumelées sont en réalité difficiles à saisir et combien il est hasardeux de définir, quel que soit l’effort rétrospectif, la critique que Steinbeck et Ford ont proposée dans leur version des Raisins de la colère, une œuvre inoubliable, déchirante mais ambiguë sur le plan politique.

  10. De Novo and Inherited Mutations in COL4A2, Encoding the Type IV Collagen α2 Chain Cause Porencephaly

    Science.gov (United States)

    Yoneda, Yuriko; Haginoya, Kazuhiro; Arai, Hiroshi; Yamaoka, Shigeo; Tsurusaki, Yoshinori; Doi, Hiroshi; Miyake, Noriko; Yokochi, Kenji; Osaka, Hitoshi; Kato, Mitsuhiro; Matsumoto, Naomichi; Saitsu, Hirotomo

    2012-01-01

    Porencephaly is a neurological disorder characterized by fluid-filled cysts or cavities in the brain that often cause hemiplegia. It has been suggested that porencephalic cavities result from focal cerebral degeneration involving hemorrhages. De novo or inherited heterozygous mutations in COL4A1, which encodes the type IV α1 collagen chain that is essential for structural integrity for vascular basement membranes, have been reported in individuals with porencephaly. Most mutations occurred at conserved Gly residues in the Gly-Xaa-Yaa repeats of the triple-helical domain, leading to alterations of the α1α1α2 heterotrimers. Here we report on two individuals with porencephaly caused by a heterozygous missense mutation in COL4A2, which encodes the type IV α2 collagen chain. Mutations c.3455G>A and c.3110G>A, one in each of the individuals, cause Gly residues in the Gly-Xaa-Yaa repeat to be substituted as p.Gly1152Asp and p.Gly1037Glu, respectively, probably resulting in alterations of the α1α1α2 heterotrimers. The c.3455G>A mutation was found in the proband's mother, who showed very mild monoparesis of the left upper extremity, and the maternal elder uncle, who had congenital hemiplegia. The maternal grandfather harboring the mutation is asymptomatic. The c.3110G>A mutation occurred de novo. Our study confirmed that abnormalities of the α1α1α2 heterotrimers of type IV collagen cause porencephaly and stresses the importance of screening for COL4A2 as well as for COL4A1. PMID:22209246

  11. Genetic and epigenetic factors at COL2A1 and ABCA4 influence clinical outcome in congenital toxoplasmosis.

    Directory of Open Access Journals (Sweden)

    Sarra E Jamieson

    Full Text Available BACKGROUND: Primary Toxoplasma gondii infection during pregnancy can be transmitted to the fetus. At birth, infected infants may have intracranial calcification, hydrocephalus, and retinochoroiditis, and new ocular lesions can occur at any age after birth. Not all children who acquire infection in utero develop these clinical signs of disease. Whilst severity of disease is influenced by trimester in which infection is acquired by the mother, other factors including genetic predisposition may contribute. METHODS AND FINDINGS: In 457 mother-child pairs from Europe, and 149 child/parent trios from North America, we show that ocular and brain disease in congenital toxoplasmosis associate with polymorphisms in ABCA4 encoding ATP-binding cassette transporter, subfamily A, member 4. Polymorphisms at COL2A1 encoding type II collagen associate only with ocular disease. Both loci showed unusual inheritance patterns for the disease allele when comparing outcomes in heterozygous affected children with outcomes in affected children of heterozygous mothers. Modeling suggested either an effect of mother's genotype, or parent-of-origin effects. Experimental studies showed that both ABCA4 and COL2A1 show isoform-specific epigenetic modifications consistent with imprinting. CONCLUSIONS: These associations between clinical outcomes of congenital toxoplasmosis and polymorphisms at ABCA4 and COL2A1 provide novel insight into the molecular pathways that can be affected by congenital infection with this parasite.

  12. Gene Editing for the Efficient Correction of a Recurrent COL7A1 Mutation in Recessive Dystrophic Epidermolysis Bullosa Keratinocytes.

    Science.gov (United States)

    Chamorro, Cristina; Mencía, Angeles; Almarza, David; Duarte, Blanca; Büning, Hildegard; Sallach, Jessica; Hausser, Ingrid; Del Río, Marcela; Larcher, Fernando; Murillas, Rodolfo

    2016-04-05

    Clonal gene therapy protocols based on the precise manipulation of epidermal stem cells require highly efficient gene-editing molecular tools. We have combined adeno-associated virus (AAV)-mediated delivery of donor template DNA with transcription activator-like nucleases (TALE) expressed by adenoviral vectors to address the correction of the c.6527insC mutation in the COL7A1 gene, causing recessive dystrophic epidermolysis bullosa in a high percentage of Spanish patients. After transduction with these viral vectors, high frequencies of homology-directed repair were found in clones of keratinocytes derived from a recessive dystrophic epidermolysis bullosa (RDEB) patient homozygous for the c.6527insC mutation. Gene-edited clones recovered the expression of the COL7A1 transcript and collagen VII protein at physiological levels. In addition, treatment of patient keratinocytes with TALE nucleases in the absence of a donor template DNA resulted in nonhomologous end joining (NHEJ)-mediated indel generation in the vicinity of the c.6527insC mutation site in a large proportion of keratinocyte clones. A subset of these indels restored the reading frame of COL7A1 and resulted in abundant, supraphysiological expression levels of mutant or truncated collagen VII protein. Keratinocyte clones corrected both by homology-directed repair (HDR) or NHEJ were used to regenerate skin displaying collagen VII in the dermo-epidermal junction.

  13. CNV analysis in Tourette syndrome implicates large genomic rearrangements in COL8A1 and NRXN1.

    Science.gov (United States)

    Nag, Abhishek; Bochukova, Elena G; Kremeyer, Barbara; Campbell, Desmond D; Muller, Heike; Valencia-Duarte, Ana V; Cardona, Julio; Rivas, Isabel C; Mesa, Sandra C; Cuartas, Mauricio; Garcia, Jharley; Bedoya, Gabriel; Cornejo, William; Herrera, Luis D; Romero, Roxana; Fournier, Eduardo; Reus, Victor I; Lowe, Thomas L; Farooqi, I Sadaf; Mathews, Carol A; McGrath, Lauren M; Yu, Dongmei; Cook, Ed; Wang, Kai; Scharf, Jeremiah M; Pauls, David L; Freimer, Nelson B; Plagnol, Vincent; Ruiz-Linares, Andrés

    2013-01-01

    Tourette syndrome (TS) is a neuropsychiatric disorder with a strong genetic component. However, the genetic architecture of TS remains uncertain. Copy number variation (CNV) has been shown to contribute to the genetic make-up of several neurodevelopmental conditions, including schizophrenia and autism. Here we describe CNV calls using SNP chip genotype data from an initial sample of 210 TS cases and 285 controls ascertained in two Latin American populations. After extensive quality control, we found that cases (N = 179) have a significant excess (P = 0.006) of large CNV (>500 kb) calls compared to controls (N = 234). Amongst 24 large CNVs seen only in the cases, we observed four duplications of the COL8A1 gene region. We also found two cases with ∼400 kb deletions involving NRXN1, a gene previously implicated in neurodevelopmental disorders, including TS. Follow-up using multiplex ligation-dependent probe amplification (and including 53 more TS cases) validated the CNV calls and identified additional patients with rearrangements in COL8A1 and NRXN1, but none in controls. Examination of available parents indicates that two out of three NRXN1 deletions detected in the TS cases are de-novo mutations. Our results are consistent with the proposal that rare CNVs play a role in TS aetiology and suggest a possible role for rearrangements in the COL8A1 and NRXN1 gene regions.

  14. CNV analysis in Tourette syndrome implicates large genomic rearrangements in COL8A1 and NRXN1.

    Directory of Open Access Journals (Sweden)

    Abhishek Nag

    Full Text Available Tourette syndrome (TS is a neuropsychiatric disorder with a strong genetic component. However, the genetic architecture of TS remains uncertain. Copy number variation (CNV has been shown to contribute to the genetic make-up of several neurodevelopmental conditions, including schizophrenia and autism. Here we describe CNV calls using SNP chip genotype data from an initial sample of 210 TS cases and 285 controls ascertained in two Latin American populations. After extensive quality control, we found that cases (N = 179 have a significant excess (P = 0.006 of large CNV (>500 kb calls compared to controls (N = 234. Amongst 24 large CNVs seen only in the cases, we observed four duplications of the COL8A1 gene region. We also found two cases with ∼400 kb deletions involving NRXN1, a gene previously implicated in neurodevelopmental disorders, including TS. Follow-up using multiplex ligation-dependent probe amplification (and including 53 more TS cases validated the CNV calls and identified additional patients with rearrangements in COL8A1 and NRXN1, but none in controls. Examination of available parents indicates that two out of three NRXN1 deletions detected in the TS cases are de-novo mutations. Our results are consistent with the proposal that rare CNVs play a role in TS aetiology and suggest a possible role for rearrangements in the COL8A1 and NRXN1 gene regions.

  15. A novel collagen/platelet-rich plasma (COL/PRP) scaffold: preparation and growth factor release analysis.

    Science.gov (United States)

    Zhang, Xiujie; Wang, Jingwei; Ren, Mingguang; Li, Lifeng; Wang, Qingwen; Hou, Xiaohua

    2016-06-01

    Platelet-rich plasma (PRP) has been widely used in clinical practice for more than 20 years because it causes the release of many growth factors. However, the burst release pattern and short release period of PRP have become obstacles to its application. An optimal controllable release system is an urgent need for researchers. This study investigated whether collagen/PRP (COL/PRP) scaffolds can serve as a vehicle for the controllable release of growth factors. We fabricated a novel scaffold that integrates PRP activated by thrombin or collagen into type I collagen. The mechanical properties, cytotoxicity, and transforming growth factor β1 (TGF-β1), platelet derived growth factor (PDGF), fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF) content were evaluated. Our results demonstrate that the COL/PRP scaffolds were not cytotoxic to L-929 fibroblasts. The PDGF and FGF content in the thrombin group was at a higher level and lasted for a long period of time. Collagen and thrombin played the same role in the release of TGF-β1 and VEGF. These data suggest that the novel COL/PRP scaffolds provide a carrier for the controllable release of growth factors and may be used in tissue- regenerative therapies.

  16. Common variants in the COL4A4 gene confer susceptibility to lattice degeneration of the retina.

    Directory of Open Access Journals (Sweden)

    Akira Meguro

    Full Text Available Lattice degeneration of the retina is a vitreoretinal disorder characterized by a visible fundus lesion predisposing the patient to retinal tears and detachment. The etiology of this degeneration is still uncertain, but it is likely that both genetic and environmental factors play important roles in its development. To identify genetic susceptibility regions for lattice degeneration of the retina, we performed a genome-wide association study (GWAS using a dense panel of 23,465 microsatellite markers covering the entire human genome. This GWAS in a Japanese cohort (294 patients with lattice degeneration and 294 controls led to the identification of one microsatellite locus, D2S0276i, in the collagen type IV alpha 4 (COL4A4 gene on chromosome 2q36.3. To validate the significance of this observation, we evaluated the D2S0276i region in the GWAS cohort and in an independent Japanese cohort (280 patients and 314 controls using D2S0276i and 47 single nucleotide polymorphisms covering the region. The strong associations were observed in D2S0276i and rs7558081 in the COL4A4 gene (Pc = 5.8 × 10(-6, OR = 0.63 and Pc = 1.0 × 10(-5, OR = 0.69 in a total of 574 patients and 608 controls, respectively. Our findings suggest that variants in the COL4A4 gene may contribute to the development of lattice degeneration of the retina.

  17. The Solar Neighborhood. XXVI. AP Col: The Closest (8.4 pc) Pre-Main-Sequence Star

    CERN Document Server

    Riedel, Adric R; Henry, Todd J; Melis, Carl; Jao, Wei-Chun; Subasavage, John P; 10.1088/0004-6256/142/4/104

    2011-01-01

    We present the results of a multi-technique investigation of the M4.5Ve flare star AP Col, which we discover to be the nearest pre-main-sequence star. These include astrometric data from the CTIO 0.9m, from which we derive a proper motion of 342.0+/-0.5 mas yr^-1, a trigonometric parallax of 119.21+/-0.98 mas (8.39+/-0.07 pc), and photometry and photometric variability at optical wavelengths. We also provide spectroscopic data, including radial velocity (22.4+/-0.3 km s^-1), lithium Equivalent Width (EW) (0.28+/-0.02 A), H-alpha EW (-6.0 to -35 A), {\\it vsini} (11+/-1 km s^-1), and gravity indicators from the Siding Spring 2.3-m WiFeS, Lick 3-m Hamilton echelle, and Keck-I HIRES echelle spectrographs. The combined observations demonstrate that AP Col is the closer of only two known systems within 10 pc of the Sun younger than 100 Myr. Given its space motion and apparent age of 12-50 Myr, AP Col is likely a member of the recently proposed ~40 Myr old Argus/IC 2391 association.

  18. Whole exome analysis identifies dominant COL4A1 mutations in patients with complex ocular phenotypes involving microphthalmia.

    Science.gov (United States)

    Deml, B; Reis, L M; Maheshwari, M; Griffis, C; Bick, D; Semina, E V

    2014-11-01

    Anophthalmia/microphthalmia (A/M) is a developmental ocular malformation defined as complete absence or reduction in size of the eye. A/M is a heterogenous disorder with numerous causative genes identified; however, about half the cases lack a molecular diagnosis. We undertook whole exome sequencing in an A/M family with two affected siblings, two unaffected siblings, and unaffected parents; the ocular phenotype was isolated with only mild developmental delay/learning difficulties reported and a normal brain magnetic resonance imaging (MRI) in the proband at 16 months. No pathogenic mutations were identified in 71 known A/M genes. Further analysis identified a shared heterozygous mutation in COL4A1, c.2317G>A, p.(Gly773Arg) that was not seen in the unaffected parents and siblings. Analysis of 24 unrelated A/M exomes identified a novel c.2122G>A, p.(Gly708Arg) mutation in an additional patient with unilateral microphthalmia, bilateral microcornea and Peters anomaly; the mutation was absent in the unaffected mother and the unaffected father was not available. Mutations in COL4A1 have been linked to a spectrum of human disorders; the most consistent feature is cerebrovascular disease with variable ocular anomalies, kidney and muscle defects. This study expands the spectrum of COL4A1 phenotypes and indicates screening in patients with A/M regardless of MRI findings or presumed inheritance pattern.

  19. Extensive Analysis of GmFTL and GmCOL Expression in Northern Soybean Cultivars in Field Conditions.

    Directory of Open Access Journals (Sweden)

    Guangyu Guo

    Full Text Available The FLOWERING LOCUS T (FT gene is a highly conserved florigen gene among flowering plants. Soybean genome encodes six homologs of FT, which display flowering activity in Arabidopsis thaliana. However, their contributions to flowering time in different soybean cultivars, especially in field conditions, are unclear. We employed six soybean cultivars with different maturities to extensively investigate expression patterns of GmFTLs (Glycine max FT-like and GmCOLs (Glycine max CO-like in the field conditions. The results show that GmFTL3 is an FT homolog with the highest transcript abundance in soybean, but other GmFTLs may also contribute to flower induction with different extents, because they have more or less similar expression patterns in developmental-, leaf-, and circadian-specific modes. And four GmCOL genes (GmCOL1/2/5/13 may confer to the expression of GmFTL genes. Artificial manipulation of GmFTL expression by transgenic strategy (overexpression and RNAi results in a distinct change in soybean flowering time, indicating that GmFTLs not only impact on the control of flowering time, but have potential applications in the manipulation of photoperiodic adaptation in soybean. Additionally, transgenic plants show that GmFTLs play a role in formation of the first flowers and in vegetative growth.

  20. Les Nymphes dans les rites du mariage

    OpenAIRE

    2013-01-01

    Le champ du mariage (où l’on rencontre à la fois les Nymphes puissances surnaturelles et les numphai humaines) peut se révéler intéressant pour croiser des données littéraires, archéologiques et épigraphiques sur le culte des Nymphes. Lors des préliminaires, ces puissances peuvent être l’objet des protéléia (sacrifices prénuptiaux) ou être associées avec la loutrophorie et le bain prénuptial. Les Nymphes n’interviennent guère pendant la journée de célébration du mariage, sauf dans des textes ...

  1. Pourfour Du Petit syndrome after interscalene block.

    Science.gov (United States)

    Santhosh, Mysore Chandramouli Basappji; Pai, Rohini B; Rao, Raghavendra P

    2013-04-01

    Interscalene block is commonly associated with reversible ipsilateral phrenic nerve block, recurrent laryngeal nerve block, and cervical sympathetic plexus block, presenting as Horner's syndrome. We report a very rare Pourfour Du Petit syndrome which has a clinical presentation opposite to that of Horner's syndrome in a 24-year-old male who was given interscalene block for open reduction and internal fixation of fracture upper third shaft of left humerus.

  2. Sådan forebygger du faldulykker

    DEFF Research Database (Denmark)

    Evron, Lotte

    2015-01-01

    Bidrag til serien 'Eksperten skriver'. Her gennemgås en kort vejledning til, hvordan man arbejder systematisk med faldforebyggelse. Ved at arbejde systematisk med dine hverdagsobservationer kan du få øje på og reagere på ændringer hos svækkede ældre borgere og på den måde forebygge faldet, før de...

  3. Autour du paradigme d’écotourisme

    Directory of Open Access Journals (Sweden)

    Anne-Laure Transler

    2008-02-01

    Full Text Available L’écotourisme ouvre un cadre conceptuel original qui permet d’envisager des démarches renouvelées de développement à partir des revenus issus de consommations touristiques mieux réfléchies et organisées, susceptibles de soutenir des initiatives de développement local s’inscrivant dans un projet global de développement durable. Variante douce du tourisme, loin du tourisme de masse, le champ de l’écotourisme s’étend aux confins du tourisme de nature, du tourisme culturel et des pratiques scientifiques et sportives de plein air. Une définition de l’écotourisme peut être proposée à partir des six points suivants : 1/ nature et culture, 2/ le bien-être des sociétés hôtes, 3/ des touristes responsables, 4/ la participation des sociétés hôtes, 5/ la durabilité, 6/ l’art de la rencontre.Ecotourism provides an original conceptual framework that enables approaches to development that are better able to provide a more equitable distribution of income, as well as better organized and thought through initiatives which are more likely to promote a holistic sustainable local development. With somewhat less negative impact than normal tourism, considerably less that mass tourism, ecotourism can encompass nature tourism, cultural tourism, scientific exploration, and even outdoor sports... A definition of ecotourism should take into account the following six points: 1/ nature and culture, 2/ the welfare of the host community, tourists acting responsibly, 3/ participation by the local community, 5 / sustainability , 6/ promotion of cultural exchanges

  4. Technostress et autres revers du travail nomade

    NARCIS (Netherlands)

    Popma, J.

    2013-01-01

    L'Internet et l'utilisation des ordinateurs portables, des téléphones mobiles et des tablettes ont accru l'importance du « travail nomade». Ce type de travail, sans contrainte de lieu ni de temps, peut entraîner, d’après ce working paper, une plus grande autonomie et une plus grande flexibilité pour

  5. Pourfour Du Petit syndrome after interscalene block

    Directory of Open Access Journals (Sweden)

    Mysore Chandramouli Basappji Santhosh

    2013-01-01

    Full Text Available Interscalene block is commonly associated with reversible ipsilateral phrenic nerve block, recurrent laryngeal nerve block, and cervical sympathetic plexus block, presenting as Horner′s syndrome. We report a very rare Pourfour Du Petit syndrome which has a clinical presentation opposite to that of Horner′s syndrome in a 24-year-old male who was given interscalene block for open reduction and internal fixation of fracture upper third shaft of left humerus.

  6. Sustainable growth, the DuPont way.

    Science.gov (United States)

    Holliday, C

    2001-09-01

    Like many manufacturers, DuPont traditionally has grown by making more and more "stuff." And its business growth has been proportional to the amount of raw materials and energy used--as well as the resulting waste and emissions from operations. Over the years, though, DuPont became aware that cheap supplies of nonrenewable resources wouldn't be endlessly available and that the earth's ecosystems couldn't indefinitely absorb the waste and emissions of production and consumption. Chad Holliday, chairman and CEO of DuPont, believes strongly in the challenge of sustainable growth and makes the business case for it: By using creativity and scientific knowledge effectively, he says, companies can provide strong returns for shareholders and grow their businesses--while also meeting the human needs of societies around the world and reducing the environmental footprint of their operations and products. In fact, a focus on sustainability can help identify new products, markets, partnerships, and intellectual property and lead to substantial business growth. Holliday describes how DuPont developed a three-pronged strategy to translate the concept of sustainability into nuts-and-bolts business practices. Focusing on integrated science, knowledge intensity, and productivity improvement, the strategy was accompanied by a new way to measure progress quantitatively. Sustainable growth should be viewed not as a program for stepped-up environmental performance but as a comprehensive way of doing business, one that delivers tremendous economic value and opens up new opportunities. Ultimately, companies will find that they can generate substantial business value through sustainability while both enhancing the quality of life around the world and protecting the environment.

  7. 腰椎间盘退变与COL9A2基因单核苷酸多态性的相关性%Correlation between lumbar disc degeneration and COL9A2 gene single nucleotide polymorphisms

    Institute of Scientific and Technical Information of China (English)

    陈涛; 黎观保; 梁科友; 贾世青

    2013-01-01

    BACKGROUND: Studies have shown that lumbar disc degeneration is closely related to genes. OBJECTIVE: To explore the relationship between COL9A2 gene single nucleotide polymorphisms (rsl2722877, rs3737820 and rs209914) and lumbar disc degeneration. METHODS: 280 patients with lumbar degenerative disc disease were selected, and 268 age- and sex-matched patients without lumbar degenerative disc disease were selected as control group. Al the patients came from Guangxi Zhuang Autonomous Region. The blood samples were col ected from the patients with lumbar degenerative disc disease and without lumbar degenerative disc disease to extract the genomic DNA and to design PCR primers and TaqMan probe for the rsl2722877 rs3737820 and rs209914 sites of COL9A2 gene. The al ele frequency and genotype frequency were analyzed with SPSS17.0 software. RESULTS AND CONCLUSION: The differences of genotype and the al ele frequency distribution of rsl2722877 rs3737820 and rs209914 sites were statistical y significant (P < 0.05). The COL9A2 gene single nucleotide polymorphisms rsl2722877, rs3737820 and rs209914 were closely related with susceptibility of lumbar degenerative disc disease in Guangxi Zhuang population.%  背景:有研究证实,腰椎间盘退变与基因有密切关系。目的:探讨 COL9A2基因单核苷酸多态性(rsl2722877、rs3737820和 rs209914)与腰椎间盘退变性疾病的关系。方法:腰椎间盘退变性疾病患者280例,年龄、性别匹配的268例非腰椎间盘退变性疾病患者作为正常对照组,均来自广西壮族。收集腰椎间盘退变性疾病患者、非腰椎间盘退变性疾病患者的血液样本,提取基因组 DNA,设计针对 COL9A2基因 rsl2722877、rs3737820和 rs209914位点的 PCR 引物、TaqMan 探针,利用 TaqMan 探针技术对 rsl2722877、rs3737820和 rs209914位点进行 PCR 荧光分型。采用 SPSS17.0软件进行等位基因频率及基因型频率分析。结果与结论:rsl2722877

  8. Quiste de colédoco. Presentación de un caso

    Directory of Open Access Journals (Sweden)

    Daniel Olivera Fajardo

    2012-06-01

    Full Text Available La dilatación congénita de las vías biliares y su incidencia es rara, se presenta un caso cada 100 000 a 150 000 nacimientos. La preocupación actual respecto a la evolución de este proceso, es que origine un colangiocarcinoma. Se presenta el caso de una adolescente de procedencia urbana, que acudió a  cuerpo de guardia de su área de salud, refiriendo que desde hacía aproximadamente 5 meses presentaba dolor  moderado en ¨la boca¨ del estómago y en la región superior derecha del abdomen que aparecía siempre después de ingerir alimentos, se aliviaba por sí solo y se acompañaba de vómitos. Su médico de familia  indicó ultrasonido abdominal, donde se observó un quiste en el hígado. Fue  trasladada al Cuerpo de Guardia del Hospital General Universitario Dr. Gustavo Aldereguía Lima, de Cienfuegos, donde fue valorada por el Servicio de Cirugía. Se decidió la realización de  tomografÍa axial computarizada de abdomen en la que se observó un quiste a nivel de la cabeza del páncreas. Se aplicó tratamiento quirúrgico. Los resultados anatomopatológicos mostraron colecistitis crónica, quiste de colédoco e inflamación crónica inespecífica.

  9. Rapport d'Activite du Service Medical - 2001/2002

    CERN Document Server

    Fassnacht, V

    2003-01-01

    Le rapport annuel de l’année 2002 fait état de l’activité du service médical et de ses conclusions en matière de santé au travail ou de conditions de travail pour les membres du personnel du CERN, c’est à dire!: les titulaires, boursiers, étudiants, utilisateurs, attachés, etc…

  10. Tribunal administratif de l'Organisation internationale du Travail (TAOIT)

    CERN Document Server

    International Labour Organization. Geneva

    Le Tribunal administratif de l'Organisation internationale du Travail connaît des requêtes formées par les fonctionnaires ou les anciens fonctionnaires du Bureau international du Travail et des quelque trente organisations qui ont reconnu sa compétence. The ILO Administrative Tribunal (ILOAT) hears complaints from serving and former officials of the International Labour Office or one of the thirty-odd intergovernmental organizations that recognise its jurisdiction.

  11. Quelques observations sur les Bryopsis du Golfe de Naples

    NARCIS (Netherlands)

    Koster, Joséphine Th.

    1941-01-01

    Evidemment la flore algologique du Golfe de Naples a été étudiée, d’assez près, tant du point de vue floristique que du point de vue écologique. Surtout FALKENBERG (1879), BERTHOLD (1882) et FUNK (1927) se sont occupés des investigations de cette nature. Il reste cependant un certain nombre de genre

  12. Christophe Brissonneau, Olivier Aubel, Fabien Ohl, L'épreuve du dopage. Sociologie du cyclisme professionnel

    OpenAIRE

    Martinache, Igor

    2011-01-01

    L'épreuve du dopage se lit comme un roman policier. A la manière d'une intrigue, la trame du livre invite à chaque fin de page à tourner la suivante pour découvrir si le cycliste va basculer dans la pratique du dopage. De fait, il ne bascule jamais. Et c'est bien là l'intérêt de l'ouvrage qui, à l'encontre de la plupart des discours sur le sujet, montre que le dopage est une pratique processuelle qui rend rigoureusement impossible toute distinction binaire entre deux états : dopé ou non. Une ...

  13. ETUDE DU COMPORTEMENT MECANIQUE DU BETON CELLULAIRE AUTOCLAVE PRODUIT EN ALGERIE

    OpenAIRE

    R BELOUETTAR; Klepaczko, J.R.; Abadlia, M.T.

    2002-01-01

    Ce travail présente une étude expérimentale du comportement mécanique du béton cellulaire autoclavé. L’étude est portée essentiellement sur une série d’essais mécaniques en compression quasistatique à différentes vitesses de déformation variables entre 10-4 s-1 et 10 s-1 et à deux états différents (état sec et état saturé d’eau). En général, l’augmentation de la vitesse de déformation donne une augmentation de la contrainte critique du béton cellulaire autoclavé. Le béton cellulaire autoclavé...

  14. Le corps du saint dans l'hagiographie du Maghreb médiéval

    Directory of Open Access Journals (Sweden)

    Nelly Amri

    2006-11-01

    Full Text Available Ce travail tente de réfléchir sur la place accordée par l’hagiographie du Maghreb médiéval au corps dans l'expérience de sainteté et dans le processus de “sanctification” d’un homme ou d’une femme, ainsi que dans la reconnaissance ou la “consécration” de cette sainteté. Il vise à repérer les infléchissements perceptibles dans les critères de sainteté et la valeur accordée par l'hagiographie à telle ou telle voie de sanctification. Il se propose également d’examiner la représentation par les contemporains du corps du saint vivant et les bénéfices qu’ils en attendent.

  15. Dysspondyloenchondromatosis (DSC) associated with COL2A1 mutation: Clinical and radiological overlap with spondyloepimetaphyseal dysplasia-Strudwick type (SEMD-S).

    Science.gov (United States)

    Merrick, Blair; Calder, Alistair; Wakeling, Emma

    2015-12-01

    Dysspondyloenchondromatosis (DSC) is a rare skeletal dysplasia characterized by enchondroma-like lesions and anisospondyly. The former leads to discrepancies in limb length, and the latter, to progressive kyphoscoliosis. Two recent cases have highlighted the genetic heterogeneity of DSC, one demonstrating the presence and, the other, the absence of a COL2A1 mutation. This may have important clinical implications, for example, screening for complications including atlanto-axial instability associated with type II collagenopathies, as well as long-term patient management. We report on a case with radiographic features of DSC with overlap into the type II collagenopathy spondyloepimetaphyseal dysplasia, Strudwick type, who was found to carry a novel heterozygous mutation in the COL2A1 gene. Testing for COL2A1 mutations should be performed in all patients with radiological features of DSC. Further research is needed to identify the underlying molecular cause in cases where no COL2A1 mutation is identified.

  16. A founder synonymous COL7A1 mutation in three Danish families with dominant dystrophic epidermolysis bullosa pruriginosa identifies exonic regulatory sequences required for exon 87 splicing

    DEFF Research Database (Denmark)

    Covaciu, C; Grosso, F; Pisaneschi, E

    2011-01-01

    a previously unrecognized translationally silent exonic COL7A1 mutation that results in skipping of exon 87 and is associated with DDEB-Pr phenotypes in several members of three apparently unrelated Danish families. A haplotype segregation study suggested a common ancestor in these kindred. Functional splicing...... shoulders. DEB-Pr is caused by either dominant (DDEB-Pr) or recessive mutations in the COL7A1 gene encoding type VII collagen (COLVII). The full spectrum of COL7A1 mutations in DEB-Pr remains elusive and the genotype-phenotype correlation is largely incomplete. Here, we report and functionally characterize...... analysis of the mutant exon by a COL7A1 minigene construct and computational prediction for splicing regulatory cis-sequences prove that the mutation alters the activity of an exonic splicing enhancer (ESE) critical for exon inclusion. These findings substantiate for the first time the involvement...

  17. La diffusion du football de haut niveau en France

    Directory of Open Access Journals (Sweden)

    Loïc RAVENEL

    1996-06-01

    Full Text Available La pratique du football de haut niveau s'est diffusée sur le territoire français selon deux modèles complémentaires: une diffusion hiérarchique privilégiant les plus grandes villes du pays; une diffusion de proximité soucieuse d'imitation et indispensable à la création des compétitions. Les dirigeants du football ont valorisé le processus par l'intégration de nouvelles aires géographiques, évitant ainsi la saturation du système.

  18. Yes-mediated phosphorylation of focal adhesion kinase at tyrosine 861 increases metastatic potential of prostate cancer cells

    NARCIS (Netherlands)

    Chatterji, T.; Varkaris, A.S.; Parikh, N.U.; Song, J.H.; Cheng, C.J.; Schweppe, R.E.; Alexander, S.; Davis, J.W.; Troncoso, P.; Friedl, P.H.; Kuang, J.; Lin, S.H.; Gallick, G.E.

    2015-01-01

    To study the role of FAK signaling complexes in promoting metastatic properties of prostate cancer (PCa) cells, we selected stable, highly migratory variants, termed PC3 Mig-3 and DU145 Mig-3, from two well-characterized PCa cell lines, PC3 and DU145. These variants were not only increased migration

  19. Radon in homes and risk of lung cancer: 13 collaborative analyses of individual data from European case-control studies;Exposition au radon dans les habitations et risque de cancer du poumon: analyse conjointe des donnees individuelles de 13 etudes cas-temoins europeennes

    Energy Technology Data Exchange (ETDEWEB)

    Darby, S.; Hill, D.; Doll, R. [Radcliffe Infirmary (United Kingdom); Auvinen, A. [Ecole de Sante Publique, Tampere (Finland); Barros Dios, J.M.; Ruano Ravina, A. [Santiago de Compostela Univ. (Spain); Baysson, H.; Tirmarche, M. [Institut de Radioprotection et de Surete Nucleaire, 92 - Fontenay aux Roses (France); Bochicchio, F. [Institut National Italien de la Sante, Rome (Italy); Deo, H. [Reading Univ. (United Kingdom); Falk, R. [Autorite suedoise de Radioprotection, Stockholm (Sweden); Forastiere, F. [Departement d' Epidemiologie, Rome (Italy); Hakama, M. [Registre du cancer finlandais, Helsinki (Finland); Heid, I.; Schaffrath Rosario, A.; Wichmann, H.E. [Centre de recherche GSF pour l' Environnement et la Sante, Neuherberg (Germany); Kreienbrock, L. [Institut de Biometrie, d' Epidemiologie et de Traitement de l' Information, Hannovre (Germany); Kreuzer, M. [Departement de Radioprotection et de Sante, Neuherberg (Germany); Lagarde, F.; Pershagen, G. [Institut de Medecine Environnementale, Stockholm (Sweden); Makelainen, I.; Ruosteenoja, E. [Autorite de Surete Nucleaire et de Radioprotection, Helsinki (Finland); Muirhead, C. [Autorite Nationale de Radioprotection, Chilton (United Kingdom); Oberaigner, W. [Tumorregister Tirol, Innsbruck (Austria); TomaBek, L. [Institut National de Radioprotection, Prague, Republique Tcheque (Czech Republic); Whitley, E. [Bristol Univ. (United Kingdom)

    2007-05-15

    Objective: To determine the risk of lung cancer associated with exposure at home to the radioactive disintegration products of naturally occurring radon gas. Design: Collaborative analysis of individual data from 13 case-control studies of residential radon and lung cancer. Setting: Nine European countries. Subjects: 7148 cases of lung cancer and 14 208 controls. Main outcome measures: Relative risks of lung cancer and radon gas concentrations in homes inhabited during the previous 5-34 years measured in becquerels (radon disintegrations per second) per cubic metre (Bq/m3) of household air. Results: The mean measured radon concentration in homes of people in the control group was 97 Bq/m3, with 11% measuring > 200 and 4% measuring > 400 Bq/m3. For cases of lung cancer the mean concentration was 104 Bq/m3. The risk of lung cancer increased by 8.4% (95% confidence interval 3.0% to 15.8%) per 100 Bq/m3 increase in measured radon (P=0.0007). This corresponds to an increase of 16% (5% to 31%) per 100 Bq/m3 increase in usual radon- that is, after correction for the dilution caused by random uncertainties in measuring radon concentrations. The dose-response relation seemed to be linear with no threshold and remained significant (P = 0.04) in analyses limited to individuals from homes with measured radon < 200 Bq/m3. The proportionate excess risk did not differ significantly with study, age, sex, or smoking. In the absence of other causes of death, the absolute risks of lung cancer by age 75 years at usual radon concentrations of 0, 100, and 400 Bq/m3 would be about 0.4%, 0.5%, and 0.7%, respectively, for lifelong non-smokers, and about 25 times greater (10%, 12%, and 16%) for cigarette smokers. Conclusions: Collectively, though not separately, these studies show appreciable hazards from residential radon, particularly for smokers and recent ex-smokers, and indicate that it is responsible for about 2% of all deaths from cancer in Europe. (author)

  20. Prognosis value of molecular sub-types in breast cancers free of ganglionary invasion after mastectomy and impact of radiotherapy; Valeur pronostique des sous-types moleculaires dans les cancers du sein indemnes d'envahissement ganglionnaire apres mastectomie et impact de la radiotherapie

    Energy Technology Data Exchange (ETDEWEB)

    Selz, J.; Stevens, D.; Jouanneau, L.; Labib, A.; Le Scodan, R. [Hopital Rene-Huguenin, Saint-Cloud (France)

    2011-10-15

    The authors report the assessment of the prognosis value in terms of local control of molecular subtypes in patients suffering from breast cancer without ganglionary invasion, and of their predictive value for adjuvant irradiation. Medical files of nearly 700 patients have been analyzed. Some had an irradiation after mastectomy, some hadn't. The distribution of molecular sub-types is indicated, and the relationship with relapse is examined. The different molecular sub-types do not allow sub-groups with high risk of relapse to be defined. Breast cancers of stage pN0 after mastectomy seem to have a very good prognosis. Short communication

  1. Locoregional recurrence in patients suffering from a triple-negative breast cancer: interest of a systematic adjuvant ganglionary irradiation; Recidives locoregionales chez les patientes atteintes d'un cancer du sein triple-negatif: interet d'une irradiation ganglionnaire adjuvante systematique?

    Energy Technology Data Exchange (ETDEWEB)

    Clerc, J.; Sunyach, M.P.; Duruisseaux, M.; Mignotte, H.; Bajard, A.; Tredan, O.; Carrie, C.; Arnaud, A. [Centre Leon-Berard, Lyon (France)

    2011-10-15

    The authors report the assessment of locoregional recurrence percentage within women suffering from a triple-negative breast cancer without ganglionary attack, in order to examine the interest of a systematic adjuvant ganglionary irradiation. Nearly 250 women have been treated for a breast cancer between 1999 and 2009 in the Leon-Berard Centre in Lyons. All had surgery followed or not by a chemotherapy and/or a radiotherapy. The locoregional recurrence rate is not very high, but the authors cannot yet be sure to systematically propose this treatment. Short communication

  2. Optimisation du renforcement parasismique du viaduc de Caronte par une analyse dynamique temporelle

    OpenAIRE

    VIVIER, Aurélie; Charles, Pascal; DAVI, Denis

    2013-01-01

    Cet article présente un exemple d'application de l'analyse temporelle dynamique à un cas concret : l'optimisation du renforcement parasismique du viaduc de Caronte. Le diagnostic a permis de converger vers une solution de renforcement avec amortisseurs. Cette étude a été l'occasion de mettre en oeuvre des méthodes d'analyse temporelle, seules rigoureusement compatibles avec l'emploi d'amortisseurs et la complexité de l'ouvrage. Des outils de prédimensionnement des caractéristiques des disposi...

  3. Construire les défis du génie civil du LHC

    CERN Document Server

    CERN. Geneva

    2007-01-01

    Dans les années 80, le CERN a vécu la formidable aventure de la construction du tunnel de 27 kilomètres de circonférence. Ces dernières années, les équipes du génie civil se sont lancées dans l’excavation d’immenses cavernes souterraines et de kilomètres de galeries pour le LHC. Ces travaux ont exigé de véritables prouesses techniques.

  4. Sp1 upregulates the proximal promoter activity of the mouse collagen α1(XI) gene (Col11a1) in chondrocytes.

    Science.gov (United States)

    Watanabe, Keijirou; Hida, Mariko; Sasaki, Takako; Yano, Hiroyuki; Kawano, Kenji; Yoshioka, Hidekatsu; Matsuo, Noritaka

    2016-02-01

    Type XI collagen is a cartilage-specific extracellular matrix, and is important for collagen fibril formation and skeletal morphogenesis. We have previously reported that NF-Y regulated the proximal promoter activity of the mouse collagen α1(XI) gene (Col11a1) in chondrocytes (Hida et. al. In Vitro Cell. Dev. Biol. Anim. 2014). However, the mechanism of the Col11a1 gene regulation in chondrocytes has not been fully elucidated. In this study, we further characterized the proximal promoter activity of the mouse Col11a1 gene in chondrocytes. Cell transfection experiments with deletion and mutation constructs indicated that the downstream region of the NF-Y binding site (-116 to +1) is also necessary to regulate the proximal promoter activity of the mouse Col11a1 gene. This minimal promoter region has no TATA box and GC-rich sequence; we therefore examined whether the GC-rich sequence (-96 to -67) is necessary for the transcription regulation of the Col11a1 gene. Luciferase assays using a series of mutation constructs exhibited that the GC-rich sequence is a critical element of Col11a1 promoter activity in chondrocytes. Moreover, in silico analysis of this region suggested that one of the most effective candidates was transcription factor Sp1. Consistent with the prediction, overexpression of Sp1 significantly increased the promoter activity. Furthermore, knockdown of Sp1 expression by siRNA transfection suppressed the proximal promoter activity and the expression of endogenous transcript of the mouse Col11a1 gene. Taken together, these results indicate that the transcription factor Sp1 upregulates the proximal promoter activity of the mouse Col11a1 gene in chondrocytes.

  5. Screening and analysis of a new mutation of COL1A1 gene in a family with osteogenesis imperfecta%一个成骨不全家系COL1A1基因的突变筛查

    Institute of Scientific and Technical Information of China (English)

    白雪; 李克秋; 任秀智; 何晓波; 王毅; 官士珍; 景亚青; 李光

    2014-01-01

    Objective To investigate mutation of COL1A1 gene and analyze the relationship between genotype and clinical phenotype in a family with osteogenesis imperfecta (OI).Methods The family history of an OI pedigree,along with clinical data,was collected.Blood samples from the proband and his families,as well as 50 normal controls,were collected.Mutation of COL1A1 gene was screened using PCR-high resolution melting (PCR-HRM) and validated by sequencing.Results PCR-HRM method showed an abnormal result in proband COL1A1 33_34 exons,which Tm was 87.7℃,in contrast to the normal control (wt) Tm of 87.9 ℃ ± 0.06℃.There was a significant difference between the proband and the normal control with the standardization curve and the difference curves.DNA sequencing showed that COL1A1 gene exons 33_34 has lost a C base (c.2321delC),which resulted in a frameshift mutation and caused an premature termination codon (UAA) at amino acid 334,i.e.,p.Pro774LeufsX334.The father and grandfather of the proband,both suffered from OI,were verified to be heterozygous for the same mutation.The same mutation was not found in 50 normal controls.Database search confirmed this to be a novel mutation.Pedigree analysis suggested that it has an autosomal dominant inheritance.The proband and patients from the family were clinically diagnosed as OI type Ⅰ.Conclusion The study has identified a novel mutation of COL1A1 gene,c.2321delC.This frameshift mutation has caused a premature stop codon and reduced collagen type Ⅰ synthesis,characterized by a lighter OI clinical phenotype.%目的 筛查1个成骨不全(osteogenesis imperfecta,OI)家系中COL1A1基因的突变,并分析基因型与临床表型的关系.方法 收集先证者及家系成员临床资料,采集先证者、随诊家属及50名正常对照的外周血标本,应用PCR-高分辨率熔解曲线(high resolution melting,HRM)分析筛查COL1A1基因突变,基因测序确定突变位点.结果 PCR-HRM分析显示,先证者COL1A1

  6. Radiosensitivity of hepatocellular carcinoma; Radiosensibilite des cancers du foie

    Energy Technology Data Exchange (ETDEWEB)

    Hennequin, C.; Quero, L.; Rivera, S. [Service de cancerologie-radiotherapie, hopital Saint-Louis, 1, avenue Claude-Vellefeaux, 75475 Paris (France)

    2011-02-15

    The frequency of hepatocellular carcinoma (HCC) is increasing in the western world and the role of radiotherapy is more and more discussed. Classically, hepatocellular carcinoma was considered as a radioresistant tumour: in fact, modern radio-biologic studies, performed on cell lines directly established from patients, showed that hepatocellular carcinoma has the same radiosensitivity than the other epithelial tumours. From clinical studies, its {alpha}/{beta} ratio has been estimated to be around 15 Gy. Radiosensitivity of normal hepatic parenchyma is now well evaluated and some accurate NTCP models are available to guide hepatic irradiation. The biology of hepatocellular carcinoma is also better described: the combination of radiotherapy and targeted therapies will be a promising approach in the near future. (authors)

  7. Progression of Alport Kidney Disease in Col4a3 Knock Out Mice Is Independent of Sex or Macrophage Depletion by Clodronate Treatment.

    Directory of Open Access Journals (Sweden)

    Munkyung Kim

    Full Text Available Alport syndrome is a genetic disease of collagen IV (α3, 4, 5 resulting in renal failure. This study was designed to investigate sex-phenotype correlations and evaluate the contribution of macrophage infiltration to disease progression using Col4a3 knock out (Col4a3KO mice, an established genetic model of autosomal recessive Alport syndrome. No sex differences in the evolution of body mass loss, renal pathology, biomarkers of tubular damage KIM-1 and NGAL, or deterioration of kidney function were observed during the life span of Col4a3KO mice. These findings confirm that, similar to human autosomal recessive Alport syndrome, female and male Col4a3KO mice develop renal failure at the same age and with similar severity. The specific contribution of macrophage infiltration to Alport disease, one of the prominent features of the disease in human and Col4a3KO mice, remains unknown. This study shows that depletion of kidney macrophages in Col4a3KO male mice by administration of clodronate liposomes, prior to clinical onset of disease and throughout the study period, does not protect the mice from renal failure and interstitial fibrosis, nor delay disease progression. These results suggest that therapy targeting macrophage recruitment to kidney is unlikely to be effective as treatment of Alport syndrome.

  8. COL4A1 mutations cause ocular dysgenesis, neuronal localization defects, and myopathy in mice and Walker-Warburg syndrome in humans.

    Directory of Open Access Journals (Sweden)

    Cassandre Labelle-Dumais

    2011-05-01

    Full Text Available Muscle-eye-brain disease (MEB and Walker Warburg Syndrome (WWS belong to a spectrum of autosomal recessive diseases characterized by ocular dysgenesis, neuronal migration defects, and congenital muscular dystrophy. Until now, the pathophysiology of MEB/WWS has been attributed to alteration in dystroglycan post-translational modification. Here, we provide evidence that mutations in a gene coding for a major basement membrane protein, collagen IV alpha 1 (COL4A1, are a novel cause of MEB/WWS. Using a combination of histological, molecular, and biochemical approaches, we show that heterozygous Col4a1 mutant mice have ocular dysgenesis, neuronal localization defects, and myopathy characteristic of MEB/WWS. Importantly, we identified putative heterozygous mutations in COL4A1 in two MEB/WWS patients. Both mutations occur within conserved amino acids of the triple-helix-forming domain of the protein, and at least one mutation interferes with secretion of the mutant proteins, resulting instead in intracellular accumulation. Expression and posttranslational modification of dystroglycan is unaltered in Col4a1 mutant mice indicating that COL4A1 mutations represent a distinct pathogenic mechanism underlying MEB/WWS. These findings implicate a novel gene and a novel mechanism in the etiology of MEB/WWS and expand the clinical spectrum of COL4A1-associated disorders.

  9. Identification of a novel COL1A1 frameshift mutation, c.700delG, in a Chinese osteogenesis imperfecta family.

    Science.gov (United States)

    Wang, Xiran; Pei, Yu; Dou, Jingtao; Lu, Juming; Li, Jian; Lv, Zhaohui

    2015-03-01

    Osteogenesis imperfecta (OI) is a family of genetic disorders associated with bone loss and fragility. Mutations associated with OI have been found in genes encoding the type I collagen chains. People with OI type I often produce insufficient α1-chain type I collagen because of frameshift, nonsense, or splice site mutations in COL1A1 or COL1A2. This report is of a Chinese daughter and mother who had both experienced two bone fractures. Because skeletal fragility is predominantly inherited, we focused on identifying mutations in COL1A1 and COL1A2 genes. A novel mutation in COL1A1, c.700delG, was detected by genomic DNA sequencing in the mother and daughter, but not in their relatives. The identification of this mutation led to the conclusion that they were affected by mild OI type I. Open reading frame analysis indicated that this frameshift mutation would truncate α1-chain type I collagen at residue p263 (p.E234KfsX264), while the wild-type protein would contain 1,464 residues. The clinical data were consistent with the patients' diagnosis of mild OI type I caused by haploinsufficiency of α1-chain type I collagen. Combined with previous reports, identification of the novel mutation COL1A1-c.700delG in these patients suggests that additional genetic and environmental factors may influence the severity of OI.

  10. Physical and linkage mapping of the human and murine genes for the [alpha]1 chain of type IX collagen (COL9A1)

    Energy Technology Data Exchange (ETDEWEB)

    Warman, M.L. (Harvard Medical School, Boston, MA (United States) Children' s Hospital/Harvard Medical School, Boston, MA (United States)); Tiller, G.E.; Polumbo, P.A. (Vanderbilt Univ. Medical Center, Nashville, TN (United States)); Seldin, M.F.; Rochelle, J.M. (Duke Univ. Medical Center, Durham, NC (United States)); Knoll, J.H.M.; Cheng, Sou De (Children' s Hospital/Harvard Medical School, Boston, MA (United States)); Olsen, B.R. (Harvard Medical School, Boston, MA (United States))

    1993-09-01

    The IX collagen, a member of the FACIT family of extracellular matrix proteins, is a heterotrimer composed of three genetically distinct [alpha] chains. The cDNAs for the human and mouse [alpha]1(IX) chains have been cloned. In this paper the authors confirm the mapping of the human COL9A1 gene to chromosome 6q12-q13 by fluorescence in situ hybridization utilizing two genomic clones which also contain short tandem repeat polymorphisms. They also report the characterization of these repeats and their incorporation into the chromosome 6 linkage map. The COL9A1 locus shows no recombination with the marker D6Z1 (Z = 27.61 at [theta] = 0) and identifies the most likely locus order of KRAS1P-[D6Z1-COL9A1]-D6S30. In addition, using an interspecific backcross panel, they have mapped murine Col9a1 to mouse chromosome 1. Together with other comparative mapping results, these data suggest that the pericentric region of human chromosome 6 is homologous to the most proximal segment of mouse chromosome 1. These data may facilitate linkage studies with COL9A1 (or col9a1) as a candidate gene for hereditary chondrodysplasias and osteoarthritis. 35 refs., 2 figs., 2 tabs.

  11. Neo-adjuvant chemoradiotherapy by 5-fluoro-uracil and oxaliplatine for the locally evolved rectum cancer: study of the toxicity and the histological response; Chimioradiotherapie neoadjuvante par 5-fluoro-uracile et oxaliplatine pour les cancers du rectum localement evolues: etude de la toxicite et de la reponse histologique

    Energy Technology Data Exchange (ETDEWEB)

    Le Scodan, R.; Miranda, O.; Henni, M.; Durdux, C.; Housset, M. [HEGP, Dept. de Radiotherapie, 75 - Paris (France); Landi, B. [HEGP, Dept. de Gastroenterologie, 75 - Paris (France); Berger, A. [HEGP, Dept. Chirurgie Viscerale, 75 - Paris (France); Dousset, B. [Hopital Cochin, Dept. de Chirurgie Viscerale, 75 - Paris (France); Brezault, C. [Hopital Cochin, Dept. Gastroenterologie, 75 - Paris (France); Cote, J.F. [HEGP, Dept. d' anatomopathologie, 75 - Paris (France)

    2006-11-15

    The neo-adjuvant chemoradiotherapy by 5-fluoro-uracil and oxaliplatine, for the locally evolved rectum cancers, has a certain efficiency in term of histological response with an acceptable acute toxicity. The study of prediction factors to the response to chemoradiotherapy is in progress. (N.C.)

  12. Automatisation partielle du traitement de la littérature grise dans le service d'information scientifique du CERN

    CERN Document Server

    Deroche, C

    1998-01-01

    L'automatisation partielle du traitement de la litterature grise est un projet ineluctable dans un service d'information scientifique aussi important que celui du CERN. Ce rapport presente l'automatis ation du rapport annuel par la detection et l'importation automatique d'informations de publication pour les articles ecrits par les physiciens travaillant au CERN et par d'autres utilisant les outils du CERN. La recherche de ces articles est effectuee dans la base de donnees scientifiques INSPEC.

  13. Trichostatin A and Genistein Enhance the Anti-proliferative Effect of Vitamin D on Prostate Cancer Cells%曲古菌素A或金雀异黄素增强维生素D对前列腺癌细胞PC-3和DU-145的生长抑制作用

    Institute of Scientific and Technical Information of China (English)

    梁伟; 郑杰

    2009-01-01

    探讨1α,25二羟维生素D3[1,25(OH)2D3]联合曲古菌素A(trichostatin A,TSA)或金雀异黄素对维生素D不敏感前列腺癌PC-3和DU-145细胞生长的影响及其作用机制.肼和流式细胞术检测显示1,25(OH)2D3与TSA联合后对PC-3细胞的生长抑制率以及1,25(OH)2D3与金雀异黄素联合后对DU-145细胞的生长抑制率均高于单独用1,25(OH)2D3.1,25(OH)2D3与TSA联合对PC-3细胞的细胞周期阻滞效果优于单独用1,25(OH)2D3,而对DU-145细胞不如单独用1,25(OH)2D3.RT-PCR结果显示1,25(OH)2D3与TSA联合用药后,PC-3细胞p21cip1 mRNA表达水平比各单独用药组高,而DU-145细胞未见明显变化.PC-3细胞中SMRTmRNA表达水平高于DU-145细胞,而DU-145细胞中CYP24 mRNA的表达水平高于PC-3细胞,TSA和金雀异黄素可分别抑制SMRT和CYP24的表达.另外ELISA结果显示金雀异黄素明显下调DU-145细胞中的CYP24表达水平.这些研究结果表明PC-3和DU-145细胞对维生素D不敏感的机制不同,TSA可增强1,25(OH)2D3对PC-3细胞的生长抑制作用,而金雀异黄素则可增强1,25(OH)2D3对DU-145细胞的生长抑制作用,这为临床治疗维生素D不敏感肿瘤提供了新的选择.

  14. Analyse quantitative du réseau hydrographique du bassin versant du Slănic (Roumanie

    Directory of Open Access Journals (Sweden)

    Florina Grecu

    2007-12-01

    Full Text Available L'étude porte sur le bassin versant du Slănic, un affluent du Buzău. Ce bassin de taille relativement réduite (433 km2 est situé dans la région des Carpates et des Subcarpates de la Courbure, région très active sur le plan sismique. Du point de vue lithologique, le bassin du Slănic correspond à des flyschs paléogènes et à des molasses mio-pliocènes. Ces roches peu résistantes ont facilité une forte érosion du relief. Actuellement, les précipitations présentent souvent une forte intensité, et elles se produisent fréquemment après des périodes de sécheresse. Elles sont ainsi très agressives et déclenchent une érosion intense. L'analyse de quelques paramètres morphométriques [grand nombre de segments de cours d'eau d'ordres 1 (5230 et 2 (944 ; densité élevée des segments par rapport à la superficie du bassin (environ 15 segments / km2 ; longueur moyenne réduite des segments d'ordre 1 (260 m par rapport à ceux d'ordre 2 (530 m] s'accorde avec des temps de concentration des eaux courts et une forte énergie des écoulements. La vitesse de réaction aux précipitations des segments d'ordres inférieurs est en outre favorisée par la répartition spatiale des pluies et par les conditions lithologiques.The study is focused on the Slănic basin which has a small size (433 km2. The hydrographical basin of Slănic, situated in the Carpathian and Subcarpathian Curvature, a very active from neotectonical viewpoint area, is a part of the Buzău basin. From a petrographic point of view, the Slănic hydrographic basin belongs to the paleogen flysch deposits (within the Carpathian sector and to the mio-pliocen molasse (within the Subcarpathian area latter formed by predominantely friable rocks which have encouraged deep fragmentation of the relief. Actual rainfalls are often very intensive, and they frequently occur after dry seasons. The analysis of morphometric parameters shows many elementary segments of first

  15. Progrès de la connaissance du Congo, du Rwanda et du Burundi de 1993 à 2008

    Directory of Open Access Journals (Sweden)

    Henri Nicolaï

    2013-03-01

    Full Text Available Cette chronique, la vingt et unième d’une série qui a commencé avec l’année 1949, couvre la période 1993-2008 et a pour objectif de faire le point sur les progrès réalisés sur la connaissance du Congo (République démocratique du Congo, du Rwanda et du Burundi, dans le domaine de la géographie mais aussi dans les domaines des sciences naturelles et des sciences humaines qui peuvent fournir des données utiles ou indispensables aux géographes. Chaque référence bibliographique, livre ou article, est accompagnée d’un bref commentaire qui en retient les éléments principaux et surtout les faits ou les idées qui intéressent particulièrement les géographes. L’article comporte onze chapitres dont les plus importants concernent le milieu naturel, la géographie de la santé, la démographie, l’histoire (y compris l’histoire récente, la vie sociale et économique des campagnes traditionnelles et modernes, le secteur informel, les aspects de la vie urbaine. Les événements dramatiques qui se sont produits dans ces territoires africains au cours des quinze dernières années ont rendu la recherche sur le terrain particulièrement difficile tant pour les chercheurs nationaux que pour les chercheurs étrangers, ce qui se traduit notamment par une part de plus en plus importante des recherches menées en milieu urbain.This paper, the 21st issue of an edition of books and paper reviews on the knowledge of three countries of former Belgian Africa (DR Congo, Rwanda, and Burundi, covers the period 1993-2008. A short text for each reference points out the facts or ideas that are useful for geographers. The paper is composed of eleven sections. The most important are coping with the natural environment, health geography, population geography, history (including recent events, social and economic life in traditional and modern rural areas, informal economy, and urban geography. During the last sixteen years, conditions for field

  16. Evolution of a highly vulnerable ice-cored moraine: Col des Gentianes, Swiss Alps

    Science.gov (United States)

    Ravanel, L.; Lambiel, C.; Oppikofer, T.; Mazotti, B.; Jaboyedoff, M.

    2012-04-01

    Rock mass movements are dominant in the morphodynamics of high mountain rock slopes and are at the origin of significant risks for people who attend these areas and for infrastructures that are built on (mountain huts, cable cars, etc.). These risks are becoming greater because of permafrost degradation and glacier retreat, two consequences of the global warming. These two commonly associated factors may affect slope stability by changing mechanical properties of the interstitial ice and modifying the mechanical constraints in these rock slopes. Between 1977 and 1979, significant works were carried out on the Little Ice Age moraine of the Tortin glacier at the Col des Gentianes (2894 m), in the Mont Fort area (Verbier, Switzerland), for the construction of a cable car station and a restaurant. Since the early 1980s, the glacier drastically retreated and the moraine became unstable: its inner slope has retreated for several meters. Various observations and geoelectric measurements indicate that significant volume of massive ice mass is still present within the moraine (ice-cored moraine). Its melting could therefore increase the instability of the moraine. Since 2007, the moraine is surveyed by terrestrial laser scanning (TLS) in order to characterize its evolution: 8 campaigns were conducted between July 2007 and October 2011. The comparison of the high resolution 3D models so obtained allowed the detection and quantification of mass movements that have affected the moraine over this period, essentially by calculating difference maps (shortest oblique distances between two models). Between July 2007 and October 2011, 7 landslides were measured, involving volumes between 87 and 1138 m3. The most important of these occurred during the summers 2009 and 2011. TLS data also allowed identifying: (i) two main areas affected by slower but sometimes substantial movements (displacements of blocks on more than 2 m during a summer period); (ii) significant deposits of

  17. A mobilidade humana na tríplice fronteira: Peru, Brasil e Colômbia

    Directory of Open Access Journals (Sweden)

    Márcia Maria de Oliveira

    2006-08-01

    Full Text Available OS MOVIMENTOS migratórios nas fronteiras amazônicas ainda são pouco abordados, tanto pela academia quanto pelas instituições que lidam com a temática da mi-gração. Há elementos novos que configuram características peculiares à mobilidade humana na tríplice fronteira Peru-Colômbia-Brasil que merecem uma abordagem mais profunda do ponto de vista dos estudos migratórios. Atualmente, há fluxos conside-ráveis de migração internacional nessa região adentrando na fronteira brasileira, desafiando o Estado brasileiro a implementar uma política migratória que consiga lidar com fenômenos, tais como a presença de peruanos em situação irregular em território brasileiro, a mobilidade dos povos indígenas nas regiões de fronteira e ainda, mais recentemente, a entrada crescente de colombianos desplazados pela guerrilha interna que pedem refúgio ao Estado brasileiro.THE MIGRATORY mobility in the Amazonian borders is less than enough investigated, neither by the academy nor by the institutions that deal with the migration question. There are new elements that depict peculiar characteristics to the human mobility in the triple border of Peru, Colombia and Brazil, that deserve a deeper investigation from the view point of the migratory studies. At the moment, it has conside-rable flows of international migration in this region moving in the Brazilian border, challenging the Brazilian State to implement a migratory policy that is able to deal with the phenomena such as the presence of Peruvians in an irregular situation in Brazilian territory, the mobility of the indigenous peoples in the border regions and still, more recently, the increasing entrance of the Colombians desplazados through the internal guerrilla who ask for shelter to the Brazilian State.

  18. Un Genevois coopte a la tete du Front islamique du salut

    CERN Document Server

    Merckling, N

    2002-01-01

    "Le Front islamique du salut (FIS), a nomme a la tete de ce parti fondamentaliste un resident genevois, Mourad Dhani, chois parmi trois candidats. Ce physicien algerien est arrive a Geneve il y a 14 ans pour travailler au CERN (1 page).

  19. LE MARCHE MONDIAL DU VIN ET LES NOUVELLES ORIENTATIONS DU MARKETING

    Directory of Open Access Journals (Sweden)

    Epuran Gheorghe

    2008-05-01

    Full Text Available Le marché mondial du vin est caractérisé par un développement sans précédent en ce qui concerne la production, mais aussi l’aire géographique qu’il comprend. La surproduction qui a tendance ŕ dégénérer en une crise est due premičrement ŕ l’apparition de nouveaux acteurs sur ce marché, c’est-ŕ-dire les pays du nouveau monde du vin: les Etats-Unis, le Chili, l’Afrique de Sud, l’Australie. Dans ce contexte, les grands producteurs traditionnels de l’Europe sont soumis ŕ une forte pression concurrentielle aussi bien sur leurs marchés qu’ŕ l’extérieur. Męme si en matičre d’image et de notoriété les vins de ces pays sont restés encore trčs connus, les pays du nouveau monde des vins ont réussi, par un marketing «ŕ l’américain», ŕ pénétrer en force sur plusieurs marchés, inclusivement sur les pays qui étaient pas traditionnellement consommateurs de vins (la Russie, la Chine, etc..

  20. ETUDE DU COMPORTEMENT MECANIQUE DU BETON CELLULAIRE AUTOCLAVE PRODUIT EN ALGERIE

    Directory of Open Access Journals (Sweden)

    R BELOUETTAR

    2002-12-01

    Full Text Available Ce travail présente une étude expérimentale du comportement mécanique du béton cellulaire autoclavé. L’étude est portée essentiellement sur une série d’essais mécaniques en compression quasistatique à différentes vitesses de déformation variables entre 10-4 s-1 et 10 s-1 et à deux états différents (état sec et état saturé d’eau. En général, l’augmentation de la vitesse de déformation donne une augmentation de la contrainte critique du béton cellulaire autoclavé. Le béton cellulaire autoclavé présente une sensibilité à la vitesse de déformation positive. La valeur du module d’élasticité est proche de la valeur standard (1.5 GPa pour un béton cellulaire autoclavé de masse volumique égale à 500-550 kg/m3.

  1. La reconstruction du sourcil par greffon composite du cuir chevelu: une astuce pour faciliter la technique

    Science.gov (United States)

    El Omari, Mounia; El Mazouz, Samir; Gharib, Noureddine; EL Abbassi, Abdallah

    2015-01-01

    Les sourcils jouent un rôle important dans l’équilibre esthétique du visage. Leur reconstruction ou ophriopoïése, après séquelle de brûlure fait partie intégrante du programme de réhabilitation de la face brûlée. Plusieurs techniques ont été décrites. Nous insistons ici sur l'intérêt d'une technique simple, à la portée de tous les chirurgiens, et dont la méthode et les résultats peuvent être améliorés par un dessin bien planifié des zones donneuse et receveuse: la greffe composite prélevée au niveau du cuir chevelu dessinée à l'aide d'un calque du sourcil controlatéral. PMID:26401195

  2. Molecular evolution and phylogenetic analysis of eight COL superfamily genes in group I related to photoperiodic regulation of flowering time in wild and domesticated cotton (Gossypium species.

    Directory of Open Access Journals (Sweden)

    Rui Zhang

    Full Text Available Flowering time is an important ecological trait that determines the transition from vegetative to reproductive growth. Flowering time in cotton is controlled by short-day photoperiods, with strict photoperiod sensitivity. As the CO-FT (CONSTANS-FLOWER LOCUS T module regulates photoperiodic flowering in several plants, we selected eight CONSTANS genes (COL in group I to detect their expression patterns in long-day and short-day conditions. Further, we individually cloned and sequenced their homologs from 25 different cotton accessions and one outgroup. Finally, we studied their structures, phylogenetic relationship, and molecular evolution in both coding region and three characteristic domains. All the eight COLs in group I show diurnal expression. In the orthologous and homeologous loci, each gene structure in different cotton species is highly conserved, while length variation has occurred due to insertions/deletions in intron and/or exon regions. Six genes, COL2 to COL5, COL7 and COL8, exhibit higher nucleotide diversity in the D-subgenome than in the A-subgenome. The Ks values of 98.37% in all allotetraploid cotton species examined were higher in the A-D and At-Dt comparison than in the A-At and D-Dt comparisons, and the Pearson's correlation coefficient (r of Ks between A vs. D and At vs. Dt also showed positive, high correlations, with a correlation coefficient of at least 0.797. The nucleotide polymorphism in wild species is significantly higher compared to G. hirsutum and G. barbadense, indicating a genetic bottleneck associated with the domesticated cotton species. Three characteristic domains in eight COLs exhibit different evolutionary rates, with the CCT domain highly conserved, while the B-box and Var domain much more variable in allotetraploid species. Taken together, COL1, COL2 and COL8 endured greater selective pressures during the domestication process. The study improves our understanding of the domestication-related genes

  3. Prognosis value of the active tumoral volume in {sup 18}F-F.D.G. for the esophagus cancer and influence of the tumor delimitation methodology; Valeur pronostique du volume tumoral actif en {sup 18}F-FDG pour le cancer de l'oesophage et influence de la methodologie de contourage de la tumeur

    Energy Technology Data Exchange (ETDEWEB)

    Hatt, M. [LaTIM Inserm U650, 29 - Brest (France); Cheze Le Rest, C. [CHU Morvan, departement de medecine nucleaire, 29 - Brest (France); Albarghach, M.N. [CHU Morvan, departement de radiotherapie, 29 - Brest (France)

    2010-07-01

    Purpose: compare the predictive value for survival and response to the treatment of the active tumor volume automatically measured on the PET with {sup 18}F-F.D.G. images by different methods to this one of S.U.V., in the esophagus cancer. Conclusions: Our results suggest that the tumor volume is a pertinent information of which prognosis value in the esophagus cancer is clearly superior to this one of S.U.V. (maximum or average), at the condition to be measured with accuracy, what Fuzzy locally adaptive Bayesian (F.L.A.B.) allows contrary to the thresholding methods. The predictive value of total glycolysis volume (T.G.V.) is still superior and it is less influenced by the method used, F.L.A.B. offering a better differentiation, for the different responses to the treatment or for survival. (N.C.)

  4. Du Yuzhou Art Exhibition Will Open in the United Nations

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    From June 5th to 15th 2007,the headquarters of the United Nations will enjoy a show time of Du Yuzhou works,where works of photograph and painting will be exhibited. Mr.Yuzhou Du,President of the China National

  5. Role of Du Channel in Treating Senile Dementia

    Institute of Scientific and Technical Information of China (English)

    Lu Fan

    2006-01-01

    @@ The author believes that senile dementia is very closely related to not only deficiency of the liver and kidney, but also Eight Extra Channels, Du Channel in particular. Nourishing the liver and kidney and clearing Du Channel in the treatment can greatly enhance the therapeutic effect.

  6. Cyber Dhaba: un kiosque internet en Inde du Nord

    CERN Multimedia

    2002-01-01

    Il y a deux ans, l'association Orient Yourself installait un PC connecté sous un banyan sacré, dans un village agricole du Rajasthan (Inde du Nord). Claire Dixsaut dirige ELSE, elle est membre fondateur de l'association Orient Yourself.

  7. Breast cancer and per-operation electron radiotherapy: a review of the first 100 patients treated at the Jules-Bordet Institute; Cancer du sein et radiotherapie peroperatoire par electrons: revue des 100 premieres patientes traitees a l'institut Jules-Bordet

    Energy Technology Data Exchange (ETDEWEB)

    Philippson, C.; Simon, S.; Vandekerkhove, C.; Veys, I.; Noterman, D.; Hertens, D.; Neubourg, F. De; Larsimont, D.; Van Houtte, P.; Nogaret, J.M. [Institutjules-Bordet, Bruxelles (Belgium)

    2011-10-15

    The authors briefly discuss the results obtained on a cohort of women treated by per-operation radiotherapy for breast cancer. This technique enables a dose equivalent to that of a conventional fractionated external radiotherapy to be delivered in a single session in the tumour bed. Moreover, a weaker dose can be delivered as a boost before a conventional irradiation. Immediate morbidity is steady. Some rare complications have been noticed. Short communication

  8. Le pari du numérique

    Directory of Open Access Journals (Sweden)

    Marie-Françoise Durand

    2011-01-01

    Full Text Available En lançant ce premier numéro du Ceriscope, le Centre de recherches et d’études internationales (CERI et l’Atelier de cartographie de Sciences Po ont conscience de relever un défi. Engagés dans l’analyse scientifique des sociétés du monde, le CERI, en tant que centre de recherche, et l’Atelier de cartographie, en sa qualité de concepteurs de cartes, entendent faire accéder un grand nombre de lecteurs à leurs productions en utilisant la gratuité d’Internet. L’avantage d’une publication en ligne par rapport au livre papier ne se résume évidemment pas à la gratuité. Elle permet en effet un usage plus souple de la lecture en autorisant, grâce à l’indexation par mots clés, une recherche à l’intérieur des textes. Elle offre ensuite une rapidité de mise à jour des connaissances qui, compte tenu de l’évolution de l’économie et des sociétés, sont vite touchées par l’obsolescence. Les contributions mises en ligne seront ainsi actualisées et de nouvelles publications pourront les développer et les enrichir au fil du temps. La publication en ligne permet enfin les réactions des lecteurs qui deviennent ainsi des « acteurs » de la publication.

  9. Le devenir actif du corps affectif

    Directory of Open Access Journals (Sweden)

    Pascal Séverac

    2005-09-01

    Full Text Available Le but de cet article est de saisir ce que signifie, pour le corps, être actif. À partir de la proposition 49 de la partie IV de l’Éthique, on propose de distinguer deux manières d’appréhender le corps – soit comme corps organique, soit comme corps affectif -, et l’on montre que la question éthique du devenir actif s’adresse à la dimension affective du corps. Il faut penser le devenir actif du corps affectif comme augmentation de son aptitude non seulement à affecter, mais aussi à être affecté. En effet, être affecté pour le corps n’est pas identique à pâtir : au contraire, plus est grande l’ouverture sensible d’un corps aux autres corps, plus est grande son activité éthique.The aim of this paper is to understand how the body can be active. With the proposition 49 of the fourth part of Ethics, two ways of conceiving of the body are distinguished : like an organic body or like an affective body. The ethics question of becoming active is about the affective body. This becoming active must be understood as increasing of the ability to affect, as well as to be affected. To be affected is different from to be passive. On the contrary, the more the body is able to be affected, the more he becomes active.

  10. Environmental behaviour and bioavailability of Depleted Uranium (DU) material

    Energy Technology Data Exchange (ETDEWEB)

    Oeh, U.; Gerstmann, U.; Schimmack, W.; Szymczak, W.; Li, W.B.; Hoellriegl, V.; Roth, P.; Paretzke, H.G. [GSF - National Research Center for Environment and Health, Inst. of Radiation Protection, Neuherberg (Germany)

    2005-07-01

    This work was performed to assess a possible health risk of depleted uranium (DU) for residents and KFOR personnel serving on the Balkans. Therefore, the environmental behaviour and bioavailability of DU material have been explored. In order to investigate the environmental impact of DU ammunition, leaching experiments were carried out. DU penetrators were buried in soil filled in columns. The soil was irrigated (16 mm/week) and the uranium isotopes {sup 238}U and {sup 235}U which were washed out and transported into the eluate were determined by inductively coupled plasma mass spectrometry (ICP-MS). After one year, an average of 1.7% of the original DU material corroded. About 40% of the corrosion products were located on the surface of the penetrator, 60% were recovered in the soil. On the other hand, only very small amounts of the DU material could be found in the eluate (about 1 ppm per year) suggesting a low solubility of DU and the corrosion products and/or a strong sorption to the soil. In another part of the study, the solubility of DU material in human body fluids was investigated to assess the bioavailability after oral intake and inhalation of DU particles. Therefore, DU corrosion products were powdered and incubated in artificial gastric juice and simulated lung fluid. About three-fourths of the DU material was dissolved in artificial gastric juice after 30 minutes. This fraction could not be increased, even when the incubation time was extended to 120 minutes. The dissolution of DU material in artificial lung fluid showed a distinct bi-phasic course with a readily soluble fraction and a fraction of very low solubility. These findings suggest that the DU corrosion products consist mainly of two types of uranium oxides, hexavalent and fast soluble compounds and tetravalent compounds with low solubility. Additional measurements with time-of-flight secondary ion mass spectrometry (TOF-SIMS) of DU corrosion material support this conclusion. The resulting

  11. Perception de la gestion du paysage

    Directory of Open Access Journals (Sweden)

    Martin Coy

    2009-03-01

    Full Text Available Complex landscape management instruments based on a dynamic, innovative land-conservation paradigm and spatial zoning to provide for graded intensity of protection and human use are being implemented in the newer generation of biosphere reserves and biosphere parks. The multifunctionality of these exemplary landscapes with sustainable development is a great challenge; it also offers considerable potential for conflict. This paper intends to demonstrate the extent to which multifunctionality has been realised in the alpine Großes Walsertal Biosphere Park, and how the local population perceive the quality of their park five years after it was created. Landscape management efforts in the Großes Walsertal were monitored by accompanying regional-economic and perceptional studies, resulting in a discussion of required future actions.Des instruments complexes de gestion du paysage, fondés sur un paradigme dynamique et innovant de conservation ainsi que sur un zonage de l’espace visant à réguler la protection du territoire et son utilisation par l’homme, ont été mis en œuvre dans la dernière génération de réserves de biosphère et de parcs. La multifonctionnalité de ces paysages exemplaires du développement durable constitue un défi majeur, mais représente également une importante source de conflits. L’objectif de cet article est de démontrer dans quelle mesure la notion de multifonctionnalité a été appliquée à la réserve de biosphère alpine de Grosse Walsertal et d’évaluer comment la population locale perçoit la qualité de son parc, cinq ans après sa création. Les efforts de gestion du paysage dans la réserve de Grosse Walsertal ont été évalués par des études économiques régionales et par des enquêtes de perception, à l’origine d’un débat sur les actions futures à mettre en place.

  12. QUELQUES RESULTATS SUR LA COMMANDE DU CHEMOSTAT

    OpenAIRE

    ROBLEDO VELOSO, GONZALO

    2006-01-01

    L'Introduction est essentiellement consacrée á dresser un aperçu de la modélisation de quelques processus biologiques dans le chemostat, á introduire les notions de base de la théorie de la comniande (commandabilité, stabilisation asymptotique, ensemble atteignable) et á montrer á quoi elles correspondent dans le cas spécifique du chemostat. Pour cela nous avons partagé l'introduction en deux parties: la premiére partie décrit le modéle de compétition dans le chemostat (section 1.1) ...

  13. Modos de ver. Du Bois y Fanon

    Directory of Open Access Journals (Sweden)

    Sandro Mezzadra

    2014-07-01

    Full Text Available The sovereignty of glance and the primacy of sight represent the Western modern culture far beyond its artistic expression, organizing its philosophical and political conceptuality. They deeply influence the way in which subjects are conceived and constructed in Western modernity. This is the point of departure of the long-distance dialogue between W. E. B. Du Bois and Frantz Fanon staged in the article. Their political thought and militancy developed against the background of experiences – such as slavery and colonialism – characterized by process of construction of subjectivities which appear as “anomalous” with regard to the “standards” set by Modernity.

  14. Gene expression profiling of breast cancer in Lebanese women

    Science.gov (United States)

    Makoukji, Joelle; Makhoul, Nadine J.; Khalil, Maya; El-Sitt, Sally; Aldin, Ehab Saad; Jabbour, Mark; Boulos, Fouad; Gadaleta, Emanuela; Sangaralingam, Ajanthah; Chelala, Claude; Boustany, Rose-Mary; Tfayli, Arafat

    2016-01-01

    Breast cancer is commonest cancer in women worldwide. Elucidation of underlying biology and molecular pathways is necessary for improving therapeutic options and clinical outcomes. Molecular alterations in breast cancer are complex and involve cross-talk between multiple signaling pathways. The aim of this study is to extract a unique mRNA fingerprint of breast cancer in Lebanese women using microarray technologies. Gene-expression profiles of 94 fresh breast tissue samples (84 cancerous/10 non-tumor adjacent samples) were analyzed using GeneChip Human Genome U133 Plus 2.0 arrays. Quantitative real-time PCR was employed to validate candidate genes. Differentially expressed genes between breast cancer and non-tumor tissues were screened. Significant differences in gene expression were established for COL11A1/COL10A1/MMP1/COL6A6/DLK1/S100P/CXCL11/SOX11/LEP/ADIPOQ/OXTR/FOSL1/ACSBG1 and C21orf37. Pathways/diseases representing these genes were retrieved and linked using PANTHER®/Pathway Studio®. Many of the deregulated genes are associated with extracellular matrix, inflammation, angiogenesis, metastasis, differentiation, cell proliferation and tumorigenesis. Characteristics of breast cancers in Lebanese were compared to those of women from Western populations to explain why breast cancer is more aggressive and presents a decade earlier in Lebanese victims. Delineating molecular mechanisms of breast cancer in Lebanese women led to key genes which could serve as potential biomarkers and/or novel drug targets for breast cancer. PMID:27857161

  15. Two Rare Mutations in the COL1A2 Gene Associate With Low Bone Mineral Density and Fractures in Iceland.

    Science.gov (United States)

    Styrkarsdottir, Unnur; Thorleifsson, Gudmar; Eiriksdottir, Berglind; Gudjonsson, Sigurjon A; Ingvarsson, Thorvaldur; Center, Jacqueline R; Nguyen, Tuan V; Eisman, John A; Christiansen, Claus; Thorsteinsdottir, Unnur; Sigurdsson, Gunnar; Stefansson, Kari

    2016-01-01

    We conducted a genome-wide association study of low bone mineral density (BMD) at the hip and spine utilizing sequence variants found through whole-genome sequencing of 2636 Icelanders. We found two rare missense mutations, p.Gly496Ala and p.Gly703Ser, in the COL1A2 gene that associate with measures of osteoporosis in Icelanders. Mutations in COL1A2 are known to cause the autosomal dominant disorder osteogenesis imperfecta. Both variants associate with low BMD and with osteoporotic fractures. p.Gly496Ala (frequency of 0.105%) shows the strongest association with low BMD at the spine (p = 1.8 × 10(-7) , odds ratio [OR] = 4.61 [95% confidence interval (CI) 2.59, 8.18]), whereas p.Gly703Ser (frequency of 0.050%) is most strongly associated with low BMD at the hip (p = 1.9 × 10(-8) , OR = 9.34 [95% CI 4.28, 20.3]). Association with fractures was p = 2.2 × 10(-5) , OR = 3.75 (95% CI 2.03, 6.93) and p = 0.0023, OR = 4.32 (95% CI 1.69, 11.1), respectively. The carriers of these variants do not have signs of osteogenesis imperfecta other than low BMD, demonstrating that similar mutations in COL1A2 can affect skeletal phenotypes in more than one way.

  16. Transcriptional repression of the Dspp gene leads to dentinogenesis imperfecta phenotype in Col1a1-Trps1 transgenic mice.

    Science.gov (United States)

    Napierala, Dobrawa; Sun, Yao; Maciejewska, Izabela; Bertin, Terry K; Dawson, Brian; D'Souza, Rena; Qin, Chunlin; Lee, Brendan

    2012-08-01

    Dentinogenesis imperfecta (DGI) is a hereditary defect of dentin, a calcified tissue that is the most abundant component of teeth. Most commonly, DGI is manifested as a part of osteogenesis imperfecta (OI) or the phenotype is restricted to dental findings only. In the latter case, DGI is caused by mutations in the DSPP gene, which codes for dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). Although these two proteins together constitute the majority of noncollagenous proteins of the dentin, little is known about their transcriptional regulation. Here we demonstrate that mice overexpressing the Trps1 transcription factor (Col1a1-Trps1 mice) in dentin-producing cells, odontoblasts, present with severe defects of dentin formation that resemble DGI. Combined micro-computed tomography (µCT) and histological analyses revealed tooth fragility due to severe hypomineralization of dentin and a diminished dentin layer with irregular mineralization in Col1a1-Trps1 mice. Biochemical analyses of noncollagenous dentin matrix proteins demonstrated decreased levels of both DSP and DPP proteins in Col1a1-Trps1 mice. On the molecular level, we demonstrated that sustained high levels of Trps1 in odontoblasts lead to dramatic decrease of Dspp expression as a result of direct inhibition of the Dspp promoter by Trps1. During tooth development Trps1 is highly expressed in preodontoblasts, but in mature odontoblasts secreting matrix its expression significantly decreases, which suggests a Trps1 role in odontoblast development. In these studies we identified Trps1 as a potent inhibitor of Dspp expression and the subsequent mineralization of dentin. Thus, we provide novel insights into mechanisms of transcriptional dysregulation that leads to DGI.

  17. Homozygosity mapping and whole exome sequencing reveal a novel homozygous COL18A1 mutation causing Knobloch syndrome.

    Directory of Open Access Journals (Sweden)

    Alireza Haghighi

    Full Text Available The aim of this study was to identify the genetic basis of a chorioretinal dystrophy with high myopia of unknown origin in a child of a consanguineous marriage. The proband and ten family members of Iranian ancestry participated in this study. Linkage analysis was carried out with DNA samples of the proband and her parents by using the Human SNP Array 6.0. Whole exome sequencing (WES was performed with the patients' DNA. Specific sequence alterations within the homozygous regions identified by whole exome sequencing were verified by Sanger sequencing. Upon genetic analysis, a novel homozygous frameshift mutation was found in exon 42 of the COL18A1 gene in the patient. Both parents were heterozygous for this sequence variation. Mutations in COL18A1 are known to cause Knobloch syndrome (KS. Retrospective analysis of clinical records of the patient revealed surgical removal of a meningocele present at birth. The clinical features shown by our patient were typical of KS with the exception of chorioretinal degeneration which is a rare manifestation. This is the first case of KS reported in a family of Iranian ancestry. We identified a novel disease-causing (deletion mutation in the COL18A1 gene leading to a frameshift and premature stop codon in the last exon. The mutation was not present in SNP databases and was also not found in 192 control individuals. Its localization within the endostatin domain implicates a functional relevance of endostatin in KS. A combined approach of linkage analysis and WES led to a rapid identification of the disease-causing mutation even though the clinical description was not completely clear at the beginning.

  18. Luminescence de transfert de charge dans les composés oxyfluorés du molybdène, du tungstène, du niobum et du tantale

    OpenAIRE

    Zhang, Hong Jie

    1993-01-01

    Non disponible; Les caractéristique de la luminescence de transfert de charge de composés oxifluorés du niobium, du tantale, du molybdène et du tungstène ont été déterminées. L'influence de la coordinence de l'élément de transition et du mode d'assemblage des polyèdres de coordination a été examinée. Contrairement aux oxydes homologués, les phases comportant des ions alcalins peuvent présenter un rendement de luminescence élevé à 300 K lorsque les groupements oxifluorés sont isolés (ex. K3NbO...

  19. Festival du rire de Genève

    CERN Multimedia

    Staff Association

    2015-01-01

    Connaissez-vous le Festival du rire de Genève ? La deuxième édition aura lieu du 25 au 28 mars 2015 au Casino-Théâtre à Carouge. Côté programmation, Marc Donnet-Monay ouvre les festivités avant trois autres soirées de folie et d’humour que nous vous laissons le soin de découvrir dans le programme : http://www.rire-geneve.ch/#programme. Réduction de 30% sur l’achat de places pour les membres du personnel du CERN. Pour cela, il suffit de se rendre sur la billetterie en ligne de notre site : www.rire-geneve.ch et d’utiliser le code promotionnel. Contacter le secrétariat de l’Association du personnel (Staff.Association@cern.ch) pour connaitre ce code promotionnel.

  20. Le kyste hydatique du cordon spermatique : une localisation exceptionnelle

    Directory of Open Access Journals (Sweden)

    Mohamed Moncef Hamdane

    2011-12-01

    Full Text Available L� hydatidose est une anthropozoonose due au developpement chez l�homme de la forme larvaire du taenia Echinococcus granulosis. La plupart des kystes hydatiques se localisent dans le foie et les poumons. Le kyste hydatique du cordon spermatique est extremement rare avec seulement 4 cas rapportes dans la litterature. Les auteurs rapportent dans cet article un nouveau cas d�hydatidose du cordon spermatique. Il s�agissait d�un homme de 40 ans qui consultait pour des douleurs scrotales evoluant depuis huit mois. L�examen clinique a mis en evidence une tumefaction mobile, inguino-scrotale, droite. L�echographie testiculaire a objective une hernie inguinale droite associee a deux kystes epididymaires bilateraux. Le patient a ete opere pour cure de son hernie avec decouverte en per-operatoire d�un kyste du cordon spermatique qui a ete reseque. L�examen anatomopathologique a conclu a une hydatidose du cordon spermatique.

  1. Taurolidine 联合X-射线对前列腺癌细胞周期进程的影响%Effectc of taurolidine in combination with X-irradiation on cell cycle progress in the DU145 cells of human prostate cancer

    Institute of Scientific and Technical Information of China (English)

    孙宝胜; 刘林林; 刘士新; 王铁君; 孙娟

    2009-01-01

    目的 观察taurolidine联合X射线照射对前列腺癌(DU145)细胞周期进程的影响.方法 通过流式细胞仪检测不同浓度的taurolidine单独或分别联合1、2和4Gy的X-射线照射对DU145细胞周期的影响.结果 50 μmol/L的taurolidine可诱导DU145细胞发生G0/G1期阻滞,和s期的降低,并呈显著的时效和量效关系,当25 μmol/L的taurolidine联合2和4Gy的X-射线照射时,细胞发生G2/M期阻滞,然而当50 μmol/L的taurolidine联合2和4Gy的X-射线照射,细胞发生G2/M期阻滞的去除.结论 taurolidine联合X射线照射导致前列腺癌细胞G2/M期阻滞的去除,可能是诱导其细胞凋亡的一个主要机制.

  2. Col2CreERT2, A MOUSE MODEL FOR A CHONDROCYTE-SPECIFIC AND INDUCIBLE GENE DELETION

    OpenAIRE

    2014-01-01

    In 2007 and 2008, we published two articles reporting a tamoxifen (TM)-inducible, chondrocyte-specific gene-targeting mouse model in which the expression of CreERT2 is driven by the type II collagen promoter (Col2CreERT2). The fusion protein is specifically expressed and translocated into the nucleus upon TM administration, which in turn triggers gene recombination. Since then, this animal model has become a powerful tool to study the molecular mechanism of skeletal development and degenerati...

  3. Virtual Visit - Colégio de Aplicação, UFPe, Brazil / 28 April 2015

    CERN Multimedia

    ATLAS Visitor Centre

    2015-01-01

    O Colégio de Aplicação da Universidade Federal de Pernambuco fará, neste dia 28 de Abril, uma visita virtual ao experimento ATLAS no CERN, Suíça. A visita será organizada no Brasil pelos professores Kátia Aparecida da Silva Aquino e Ricardo Lima e, na Suíça, pelo professor Denis Oliveira Damazio (BNL) e apresentará o CERN aos alunos do ensino médio.

  4. The role of genes AMPD1, CNB and COL1A1 in the propensity to employment rowing.

    Directory of Open Access Journals (Sweden)

    Kozyrev A.V.

    2011-04-01

    Full Text Available The question of the frequency distribution of polymorphic alleles of genes AMPD1, CNB and COL1A1. In the experiment, was attended by athletes, rowers qualifications at the age of 21 to 28 years, and people without experience of regular sport at the age of 20 to 22 years. Established that certain combinations of alleles can be recommended as a diagnostic complex genetic markers to assess the propensity to develop and display of strength, endurance and speed. It is shown that upon receipt of positive results may conduct a successful selection in rowing, the implementation of the individualization of the training process and improve its efficiency.

  5. Bilateral vitreous hemorrhage in a newborn with Stickler syndrome associated with a novel COL2A1 mutation.

    Science.gov (United States)

    Gerth-Kahlert, Christina; Grisanti, Salvatore; Berger, Eike; Höhn, Rene; Witt, Gabriele; Jung, Ursula

    2011-06-01

    Bilateral preretinal and vitreous hemorrhages in infants are rare and can present a diagnostic challenge, with nonaccidental trauma included in the differential diagnosis. We present the case of a 4-week-old boy in which a Pierre Robin sequence and a positive family history led to the clinical diagnosis of Stickler syndrome, which was confirmed by the identification of a disease-causing novel deletion of 2 nucleotides in the COL2A1 gene. This early association with Stickler syndrome has not been described previously.

  6. Tamponamento das lesões hepáticas transfixantes com colágeno tipo I

    Directory of Open Access Journals (Sweden)

    Mantovani Mario

    1998-01-01

    Full Text Available Nos últimos seis anos temos utilizado o balão de SANGESTAKEN-BLACKMORE para o tamponamento de lesões transfixantes hepáticas. Para substituir este corpo estranho, consideramos o uso de colágeno tipo I produzido na UNICAMP, o qual pode ser injetado e moldado ocupando toda a área lesada e o trajeto anfractuoso ou irregular. Desta forma, pode-se conseguir um tamponamento completo, coibindo a hemorragia e sustando a perda de bile. O colágeno será posteriormente reabsorvido não funcionando como corpo estranho. Vinte cães foram submetidos a lesão transfixante na região central do fígado, procurando simular uma lesão produzida por um projétil de arma de fogo. Quatro destes animais, não receberam tratamento e foram a óbito por choque hemorrágico. Em todos os outros 16 animais, aplicou-se o colágeno tipo I em toda a extensão do túnel formado pela lesão, através de injeção do material. Foram realizadas avaliações clínicas no pós-operatório, sendo os cães sacrificados, em grupo de quatro, aos quatro, sete, 15 e 30 dias de pós-operatório. O estudo macroscópico e o microscópico, tanto à microscopia óptica quanto à de polarização, revelou uma formação cicatricial homogênea, com regeneração hepática intensa, precoce e organizada, a partir do 7º dia de pós-operatório. Conclui-se que a aplicação do colágeno tipo I, nestes tipos de ferimentos, são de aplicação simples e segura.

  7. Estudo da vasculopatia pulmonar no modelo experimental de esclerodermia induzido pelo colágeno do tipo V

    OpenAIRE

    Roberta Gonçalves Marangoni

    2011-01-01

    A esclerodermia sistêmica (ES) é caracterizada por fibrose da pele e órgãos internos, ativação imunológica e vasculopatia. Os modelos animais de ES sofrem com a falta de uma prova definitiva para o envolvimento vascular observado na doença humana. Um novo modelo induzido por colágeno do tipo V (COLV) reproduz muitas características da ES como fibrose e fenômenos imunológicos. No entanto, o estudo da vasculopatia ainda não foi abordado. O objetivo desse estudo foi investigar as alterações estr...

  8. Control del colapso del colágeno: desproteinización Control of the collagen network collapse: collagen remoral

    OpenAIRE

    E Osario Ruiz

    2004-01-01

    La penetración de la resina y su reacción con los componentes de los tejidos dentales desmineralizados es el mecanismo micromecánico con el cual los materiales restauradores de resina se unen al diente. Los monómeros penetran en la dentina acondicionada y se concentran en la región superficial contribuyendo a la unión. El refuerzo de esta zona con resina se conoce como hibridación, e implica la impregnación de la red de colágeno y el encapsulamiento de los cristales de hidroxiapatita. Han sur...

  9. Characterization of drCol 15a1b: a novel component of the stem cell niche in the zebrafish retina.

    Science.gov (United States)

    Gonzalez-Nunez, Veronica; Nocco, Valentina; Budd, Aidan

    2010-08-01

    There is a clear need to develop novel tools to help improve our understanding of stem cell biology, and potentially also the utility of stem cells in regenerative medicine. We report the cloning, functional, and bioinformatic characterization of a novel stem cell marker in the zebrafish retina, drCol 15a1b. The expression pattern of drCol 15a1b is restricted to stem cell niches located in the central nervous system, whereas other collagen XVs are associated with muscle and endothelial tissues. Knocking down drCol 15a1b expression causes smaller eyes, ear defects, and brain edema. Microscopic analysis reveals enhanced proliferation in the morphant eye, with many mitotic nuclei located in the central retina, together with a delayed differentiation of the mature retinal cell types. Besides, several markers known to be expressed in the ciliary marginal zone display broader expression areas in morpholino-injected embryos, suggesting an anomalous diffusion of signaling effectors from the sonic hedgehog and notch pathways. These results indicate that drCol 15a1b is a novel stem cell marker in the central nervous system that has a key role in homing stem cells into specialized niches in the adult organism. Moreover, mutations in the hCol 18a1 gene are responsible for the Knobloch syndrome, which affects brain and retinal structures, suggesting that drCol 15a1b may function similarly to mammalian Col 18a1. Thus, our results shed new light on the signaling pathways that underlie the maintenance of stem cells in the adult organism while helping us to understand the role of extracellular matrix proteins in modulating the signals that determine stem cell differentiation, cell cycle exit and apoptosis.

  10. A novel COL4A1 frameshift mutation in familial kidney disease: the importance of the C-terminal NC1 domain of type IV collagen

    Science.gov (United States)

    Gale, Daniel P.; Oygar, D. Deren; Lin, Fujun; Oygar, P. Derin; Khan, Nadia; Connor, Thomas M.F.; Lapsley, Marta; Maxwell, Patrick H.; Neild, Guy H.

    2016-01-01

    Background Hereditary microscopic haematuria often segregates with mutations of COL4A3, COL4A4 or COL4A5 but in half of families a gene is not identified. We investigated a Cypriot family with autosomal dominant microscopic haematuria with renal failure and kidney cysts. Methods We used genome-wide linkage analysis, whole exome sequencing and cosegregation analyses. Results We identified a novel frameshift mutation, c.4611_4612insG:p.T1537fs, in exon 49 of COL4A1. This mutation predicts truncation of the protein with disruption of the C-terminal part of the NC1 domain. We confirmed its presence in 20 family members, 17 with confirmed haematuria, 5 of whom also had stage 4 or 5 chronic kidney disease. Eleven family members exhibited kidney cysts (55% of those with the mutation), but muscle cramps or cerebral aneurysms were not observed and serum creatine kinase was normal in all individuals tested. Conclusions Missense mutations of COL4A1 that encode the CB3 [IV] segment of the triple helical domain (exons 24 and 25) are associated with HANAC syndrome (hereditary angiopathy, nephropathy, aneurysms and cramps). Missense mutations of COL4A1 that disrupt the NC1 domain are associated with antenatal cerebral haemorrhage and porencephaly, but not kidney disease. Our findings extend the spectrum of COL4A1 mutations linked with renal disease and demonstrate that the highly conserved C-terminal part of the NC1 domain of the α1 chain of type IV collagen is important in the integrity of glomerular basement membrane in humans. PMID:27190376

  11. Rejets et environnement du CERN - 2005

    CERN Document Server

    Vojtyla, P

    2006-01-01

    La radioactivité de l’environnement autour de l’Organisation Européenne pour la Recherche Nucléaire (CERN) et les doses de rayonnements qui en résultent pour la population avoisinante sont contrôlées par la Commission de Sécurité du CERN et de manière indépendante par les autorités compétentes des deux États Hôtes, l’Institut de Radioprotection et de Sûreté Nucléaire (IRSN) côté France et l’Office Fédéral de la Santé Publique (OFSP) côté Suisse. Dans ce rapport, les résultats de mesures concernent en particulier le territoire suisse. L’ensemble des mesures effectuées en 2005 prouve que le fonctionnement des installations du CERN était sans conséquence radiologique sur l’environnement et la population. Le contrôle des émissions montre que les rejets effectifs se situent également en 2005 nettement en dessous des limites réglementaires. Ce constat est confirmé par le contrôle des immissions dans l’environnement. Le CERN a bien respecté en 2005 comme dans les ann�...

  12. La mathématique du physicien

    CERN Document Server

    Diu, Bernard

    2012-01-01

    " Le livre de la nature est écrit dans la langue mathématique, et ses caractères sont des triangles, des cercles et autres figures géométriques, sans lesquels il est humainement impossible d'en comprendre le moindre mot ". Ce célèbre aphorisme de Galilée a scellé l'alliance des mathématiques et de la physique. Et pourtant, trois siècles plus tard, Einstein se montrait plus sceptique. Dans ce livre, Bernard Diu montre que, en effet, si les mathématiques sont un instrument indispensable de la physique, elles n'en constituent pas le fondement. En multipliant les exemples, il marque la différence entre la mathématique du mathématicien et celle du physicien. Un livre de nature à remettre en question l'enseignement de la physique dans nos écoles et des sciences en général, soumis à l'hégémonie des mathématiques.

  13. Du Bois的对话句法

    Institute of Scientific and Technical Information of China (English)

    高彦梅

    2015-01-01

    日常口语交流过程中,人们会自觉不自觉地复用即时语境中前面说话人或历史上前人的词语、短语、句法结构等成分。这样便在自己和前面说话人的话语之间唤起了共鸣。Du Bois在Bakhtin的对话理论、Jakobson的平行理论和Harris的话语分析程序的启发之下,依据自然口语语料、从社会认知角度发展了对话句法理论,目的在于揭示人类交流过程中符号与符号之间并通过符号表达的介入结构。本文综合Du Bois以及相关学者过去三十年间对对话句法的研究,呈现对话句法理论的主体脉络。

  14. Conformal radiotherapy with intensity modulation and integrated boost in the head and neck cancers: experience of the Curie Institute; Radiotherapie conformationnelle avec modulation d'intensite et boost integre des cancers de la tete et du cou: experience de l'institut Curie

    Energy Technology Data Exchange (ETDEWEB)

    Toledano, I.; Serre, A.; Bensadoun, R.J.; Ortholan, C.; Racadot, S.; Calais, G.; Alfonsi, M.; Giraud, P. [Centre Jean-Perrin, 63 - Clermont-Ferrand (France); Graff, P.; Serre, A.; Bensadoun, R.J.; Ortholan, C.; Racadot, S.; Calais, G.; Alfonsi, M.; Giraud, P. [Hopital Europeen Georges-Pompidou, 75 -Paris (France)

    2009-10-15

    The modulated intensity radiotherapy (I.M.R.T.) is used in the treatment of cancers in superior aero digestive tracts to reduce the irradiation of parotids and to reduce the delayed xerostomia. This retrospective study presents the results got on the fourteen first patients according an original technique of I.M.R.T. with integrated boost. It appears that this technique is feasible and allows to reduce the xerostomia rate without modifying the local control rate. To limit the average dose to the parotids under 30 Gy seems reduce the incidence of severe xerostomia. (N.C.)

  15. Torres Colón Madrid – España

    Directory of Open Access Journals (Sweden)

    Lamela, A.

    1977-09-01

    Full Text Available The Columbus Towers have introduced a new structural system in Spain which, up to now, has only been applied in 17 cases throughout the world; we refer to the suspended structure. Due to its location, at the intersection of our important Madrid streets, and to its unusual building process, the building has attracted the attention of technicians and public alike. The much discussed difference in the cost between this procedure and conventional methods, estimated to be about 20%, is widely justified and compensated in this case, to such an extent that it can be considered as the only possible functional solution in view of the site's reduced size and irregular characteristics in addition to the great height at which the Towers were planned and the lack of space available on the ground floors. The hanging structure, supported exclusively by means of a central concrete nucleus, permitted installation of the ramps and parking space in the basement, which would have been practically impossible to carry out using traditional construction methods and it freed the ground floors from structural conditioners in the interior distribution, providing the necessary clear space to carry out a flexible and varied distribution plan, as required by the characteristics of the offices, for which the building was designed.Torres Colón han introducido en España un nuevo sistema estructural, sólo aplicado hasta el momento en 17 casos en todo el mundo: el de la estructura colgada. Por su situación, en la confluencia de cuatro importantes vías madrileñas, y por las peculiaridades de su proceso constructivo, el edificio ha atraído la atención de los técnicos y del público no especializado. La muy discutida diferencia de costes entre este procedimiento y el tradicional, estimada aproximadamente en un 10-15%, queda ampliamente justificada y compensada en este caso, hasta tal punto, que puede ser considerada como la única solución funcional posible, dada la

  16. Parathyroid hormone related-protein promotes epithelial-to-mesenchymal transition in prostate cancer.

    Directory of Open Access Journals (Sweden)

    Weg M Ongkeko

    Full Text Available Parathyroid hormone-related protein (PTHrP possesses a variety of physiological and developmental functions and is also known to facilitate the progression of many common cancers, notably their skeletal invasion, primarily by increasing bone resorption. The purpose of this study was to determine whether PTHrP could promote epithelial-to-mesenchymal transition (EMT, a process implicated in cancer stem cells that is critically involved in cancer invasion and metastasis. EMT was observed in DU 145 prostate cancer cells stably overexpressing either the 1-141 or 1-173 isoform of PTHrP, where there was upregulation of Snail and vimentin and downregulation of E-cadherin relative to parental DU 145. By contrast, the opposite effect was observed in PC-3 prostate cancer cells where high levels of PTHrP were knocked-down via lentiviral siRNA transduction. Increased tumor progression was observed in PTHrP-overexpressing DU 145 cells while decreased progression was observed in PTHrP-knockdown PC-3 cells. PTHrP-overexpressing DU 145 formed larger tumors when implanted orthoptopically into nude mice and in one case resulted in spinal metastasis, an effect not observed among mice injected with parental DU 145 cells. PTHrP-overexpressing DU 145 cells also caused significant bone destruction when injected into the tibiae of nude mice, while parental DU 145 cells caused little to no destruction of bone. Together, these results suggest that PTHrP may work through EMT to promote an aggressive and metastatic phenotype in prostate cancer, a pathway of importance in cancer stem cells. Thus, continued efforts to elucidate the pathways involved in PTHrP-induced EMT as well as to develop ways to specifically target PTHrP signaling may lead to more effective therapies for prostate cancer.

  17. Avaliação do custo de colírios lubrificantes a base exclusivamente de carboximetilcelulose no mercado brasileiro

    Directory of Open Access Journals (Sweden)

    Paulo Estacia

    2013-10-01

    Full Text Available OBJETIVO: Avaliar o custo do uso dos colírios lubrificantes mais utilizados no mercado nacional que apresentam na formulação o mesmo princípio ativo - carboximetilcelulose, considerando o número total de gotas presente em cada frasco dos colírios. MÉTODOS: Foi realizado um estudo experimental utilizando três frascos de cada um dos colírios Lacrifilm® (colírio 1 e Fresh Tears® (colírio 2. Para análise do custo dos colírios contou-se o número de gotas de cada frasco correlacionando os preços dos mesmos. O preço considerado para cada medicação foi o mínimo ao consumidor com alíquota de 17% publicado para o mês de janeiro de 2012. A análise estatística foi efetuada em SPSS® 18. A comparação das variáveis quantitativas analisadas foi procedida através do teste não paramétrico Mann-Whitney e correlação linear de Spearman, sendo considerada uma diferença estatisticamente significante um valor de probabilidade inferior a 0,05. RESULTADOS: Verificou-se que há diferença estatisticamente significativa entre as marcas de colírios avaliados com relação ao número total de gotas. O colírio 1 apresentou o maior número de gotas. CONCLUSÃO: O colírio 1, que tem o maior número de gotas por frasco, também é o que tem o preço mais acessível. Portanto, verifica-se que é o produto mais econômico, ou seja, o paciente pagará menos por cada gota.

  18. Microarray-based detection and expression analysis of extracellular matrix proteins in drug‑resistant ovarian cancer cell lines.

    Science.gov (United States)

    Januchowski, Radosław; Zawierucha, Piotr; Ruciński, Marcin; Zabel, Maciej

    2014-11-01

    Ovarian cancer is the most lethal gynecological malignancy. Multiple drug resistance (MDR) development leads to resistance of cancer cells to chemotherapy. Microarray methods can provide information regarding new candidate genes that can play a role in resistance to cytostatic drugs. Extracellular matrix (ECM) can influence drug resistance by inhibiting the penetration of the drug into cancer tissue as well as increased apoptosis resistance. In the present study, we report changes in the ECM and related gene expression pattern in methotrexate-, cisplatin-, doxorubicin-, vincristine-, topotecan- and paclitaxel-resistant variants of the W1 ovarian cancer cell line. The resistant variants of the W1 cell line were generated by stepwise selection of cells with an increasing concentration of the indicated drugs. Affymetrix GeneChip® Human Genome U219 Array Strips were used for hybridizations. Independent t-tests were used to determinate the statistical significance of results. Genes whose expression levels were higher than the assumed threshold (upregulated, >5-fold and downregulated, 20-fold. These genes were: ITGB1BP3, COL3A1, COL5A2, COL15A1, TGFBI, DCN, LUM, MATN2, POSTN and EGFL6. The expression of seven genes decreased very significantly: ITGA1, COL1A2, LAMA2, GPC3, KRT23, VIT and HMCN1. The expression pattern of ECM and related genes provided the preliminary view into the role of ECM components in cytostatic drug resistance of cancer cells. The exact role of the investigated genes in drug resistance requires further investigation.

  19. Osteogenesis imperfecta type I: Molecular heterogeneity for COL1A1 null alleles of type I collagen

    Energy Technology Data Exchange (ETDEWEB)

    Willing, M.C.; Deschenes, S.P.; Pitts, S.H.; Arikat, H.; Roberts, E.J.; Scott, D.A.; Slayton, R.L. [Univ. of Iowa, Iowa City, IA (United States); Byers, P.H. [Univ. of Washington, Seattle, WA (United States)

    1994-10-01

    Osteogenesis imperfecta (OI) type I is the mildest form of inherited brittle-bone disease. Dermal fibroblasts from most affected individuals produce about half the usual amount of type I procollagen, as a result of a COL1A1 {open_quotes}null{close_quotes} allele. Using PCR amplification of genomic DNA from affected individuals, followed by denaturing gradient gel electrophoresis (DGGE) and SSCP, we identified seven different COL1A1 gene mutations in eight unrelated families with OI type I. Three families have single nucleotide substitutions that alter 5{prime} donor splice sites; two of these unrelated families have the same mutation. One family has a point mutation, in an exon, that creates a premature termination codon, and four have small deletions or insertions, within exons, that create translational frameshifts and new termination codons downstream of the mutation sites. Each mutation leads to both marked reduction in steady-state levels of mRNA from the mutant allele and a quantitative decrease in type I procollagen production. Our data demonstrate that different molecular mechanisms that have the same effect on type I collagen production result in the same clinical phenotype. 58 refs., 4 figs., 1 tab.

  20. Metabolic footprint analysis uncovers strain specific overflow metabolism and D-isoleucine production of Staphylococcus aureus COL and HG001.

    Science.gov (United States)

    Dörries, Kirsten; Lalk, Michael

    2013-01-01

    During infection processes, Staphylococcus aureus is able to survive within the host and to invade tissues and cells. For studying the interaction between the pathogenic bacterium and the host cell, the bacterial growth behaviour and its metabolic adaptation to the host cell environment provides first basic information. In the present study, we therefore cultivated S. aureus COL and HG001 in the eukaryotic cell culture medium RPMI 1640 and analyzed the extracellular metabolic uptake and secretion patterns of both commonly used laboratory strains. Extracellular accumulation of D-isoleucine was detected starting during exponential growth of COL and HG001 in RPMI medium. This non-canonical D-amino acid is known to play a regulatory role in adaptation processes. Moreover, individual uptake of glucose, accumulation of acetate, further overflow metabolites, and intermediates of the branched-chain amino acid metabolism constitute unique metabolic footprints. Altogether these time-resolved footprint analyses give first metabolic insights into staphylococcal growth behaviour in a culture medium used for infection related studies.