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Sample records for cana-de-acucar diatraea saccharalis

  1. Reproduction of Trichospilus diatraeae in Diatraea saccharalis after three generations in Tenebrio molitor

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    Daniele Fabiana Glaeser

    2014-06-01

    Full Text Available The successive rearing of parasitoids in factitious hosts may affect its biological quality. Trichospilus diatraeae Cherian & Margabandhu, 1942 (Hymenoptera: Eulophidae has been studied for the biological control of sugarcane borer [Diatraea saccharalis (Fabricius, 1794 (Lepidoptera: Crambidae]. This study aimed to evaluate whether the rearing of T. diatraeae for three generations in the factitious host Tenebrio molitor Linnaeus, 1758 (Coleoptera: Tenebrionidae affects its reproductive performance, when subsequently reared in the natural host pupae D. saccharalis. Two groups of T. diatraeae were reared separately for three generations: one in pupae of T. molitor and the other in pupae of D. saccharalis. Subsequently, 20 pupae of D. saccharalis were exposed, for 72 hours, to the parasitism of T. diatraeae females reared earlier in pupae of T. molitor or D. saccharalis. The successive rearing of T. diatraeae in the factitious host did not affect the number of pupae parasitized and the number of pupae in which the emergence of parasitoids occurred in the natural host D. saccharalis, and increased the longevity of females and the sex ratio of T. diatraeae. The progeny, duration of developmental cycle (egg to adult, width of head capsule of males and females and longevity of males of T. diatraeae were similar on both treatments. T. diatraeae can be reared in the factitious host T. molitor for three generations without compromising its reproductive performance, when subsequently reared in the natural host D. saccharalis.

  2. SEARCHING AND PARASITISM OF Diatraea saccharalis (LEPIDOPTERA: CRAMBIDAE BY Trichospilus diatraeae (HYMENOPTERA: EULOPHIDAE

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    Elizangela Leite Vargas

    2013-05-01

    La capacidad de Trichospilus diatraeae Cherian y Margabandhu, 1942 (Hymenoptera: Eulophidae para buscar y parasitar las pupas de Diatraea saccharalis (Fabricius, 1794 (Lepidoptera: Crambidae en los tallos de la caña de azúcar fue estudiada. Para analizar la habilidad de búsqueda y parasitismo fueron utilizados tallos de la caña de azúcar (20 cm donde se introdujo una pupa de D. saccharalis (T1; pupas y orugas (T2 o pupa y residuos fecales (T3. Cada tallo fue colocado en una botella plástica transparente con 21 hembras de T. diatraeae. Esas pupas fueron individualizadas, luego de 72 h, en tubos de vidrio a 25 ± 1°C, 70 ± 10 % UR y 14 h de foto período. El experimento se desarrolló en un diseño completamente al azar, con tres tratamientos y 12 repeticiones. Los porcentajes de pupas de D. saccharalis parasitados por T. diatraeae fueron del 50,00 %, 83,33 % y 16,66 % en el T1, T2 y T3, respectivamente (c2 = 3.896, p = 0,04. La presencia de D. saccharalis en los tallos de caña de azúcar favorecieron la búsqueda y parasitismo de su hospedero.

  3. Biological characteristics of Trichospilus diatraeae (Hymenopetra: Eulophidae in the hosts Bombyx mori (Lepidoptera: Bombycidae and Diatraea saccharalis (Lepidoptera: Crambidae

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    Vanessa Rodrigues Ferreira Calado

    2014-03-01

    Full Text Available Trichospilus diatraeae Cherian & Margabandhu, 1942 (Hymenoptera: Eulophidae is a pupal endoparasitoid of lepidoptera and it has been studied as a potential agent for biological control of pests. For developing techniques to breed parasitoids, there is a need to choose the appropriate alternative host, thus, this article aims to evaluate the biological characteristics of T. diatraeae with regard to the hosts Bombyx mori Linneaus (Lepidoptera: Bombycidae and Diatraea saccharalis (Fabricius (Lepidoptera: Crambidae, in laboratory. Twelve pupae of B. mori and twelve pupae of D. saccharalis, within 72 and 24 h of life, respectively, were exposed to parasitism by 21 female parasitoids at 25 ± 1°C, with relative humidity 70 ± 10% and photophase of 14 h. Life-cycle duration (egg – adult of T. diatraeae was 19.44 ± 0.12 days in pupae of D. saccharalis and 18.00 ± 0.05 days in pupae of B. mori, the emergence of parasitoid progeny was 66.60% in pupae of D. saccharalis, and 75.00% in pupae of B. mori. The progeny of T. diatraeae was 354.50 ± 43.21 per pupa of D. saccharalis and 469.11 ± 15.19 per pupa of B. mori. Trichospilus diatraeae showed suitability to the host and its ability to parasitize various hosts.

  4. Toxicidade de inseticidas a Diatraea saccharalis (Fabr., 1794) (Lepidoptera:Crambidae) e Cotesia flavipes (Cameron, 1891) (Hymenoptera: Braconidae)

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    Edgar Francisco Gaona Mena

    2010-01-01

    A magnitude do problema de Diatraea saccharalis (Fabricius) tem aumentado nos últimos anos, principalmente nas culturas de cana-de-açúcar e milho no Brasil. Tradicionalmente, D. saccharalis tem sido controlado com agentes de controle biológico, principalmente na cultura da cana-de-açúcar. No entanto, a intervenção química tem sido necessária para o controle de D. saccharalis em ambas as culturas. Visando estabelecer estratégias proativas para o manejo da resistência de D. saccharalis a inseti...

  5. Impact of terbufos on Cotesia flavipes, a parasitoid of Diatraea saccharalis

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    Vargas, E.; Valverde, B.; Carazo, E.

    1999-01-01

    The effect of terbufos on larvae of Diatraea saccharalis and its parasitoid, Cotesia flavipes was evaluated in the laboratory. Bioassays were conducted to determine the dose response of non-parasitized larvae of D. saccharalis feeding on artificial diet contaminated with terbufos. From the dose-response curve based on larval fresh weight, sublethal doses ranging from 1.32 ppm to 108 ppm of terbufos were selected for further studying the effect of the insecticide on both species. Both parasitized and non-parasitized larvae were exposed to the selected sublethal doses of terbufos in the diet. Consumption of the insecticide by the host resulted in mortality of the parasitoid, increased length of its larval and pupal periods, decreased adult fresh weight and changes in sex proportions. These negative effects were more severe as the dose of the insecticide increased. D. saccharalis was also affected by terbufos; larvae showed abnormalities, the length of the larval and pupal periods increased and the proportion of the females was reduced. In a preliminary greenhouse bioassay, only traces of terbufos or its metabolites were found in treated maize plants and in tissue of D. saccharalis larvae feeding on them. (author)

  6. Critical electrolyte concentration of silk gland chromatin of the sugarcane borer Diatraea saccharalis, induced using agrochemicals.

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    Santos, S A; Fermino, F; Moreira, B M T; Araujo, K F; Falco, J R P; Ruvolo-Takasusuki, M C C

    2014-09-29

    The sugarcane borer Diatraea saccharalis is widely known as the main pest of sugarcane crop, causing increased damage to the entire fields. Measures to control this pest involve the use of chemicals and biological control with Cotesia flavipes wasps. In this study, we evaluated the insecticides fipronil (Frontline; 0.0025%), malathion (Malatol Bio Carb; 0.4%), cipermetrina (Galgotrin; 10%), and neem oil (Natuneem; 100%) and the herbicide nicosulfuron (Sanson 40 SC; 100%) in the posterior region silk glands of 3rd- and 5th-instar D. saccharalis by studying the variation in the critical electrolyte concentration (CEC). Observations of 3rd-instar larvae indicated that malathion, cipermetrina, and neem oil induced increased chromatin condensation that may consequently disable genes. Tests with fipronil showed no alteration in chromatin condensation. With the use of nicosulfuron, there was chromatin and probable gene decompaction. In the 5th-instar larvae, the larval CEC values indicated that malathion and neem oil induced increased chromatin condensation. The CEC values for 5th-instar larvae using cipermetrina, fipronil, and nicosulfuron indicated chromatin unpacking. These observations led us to conclude that the quantity of the pesticide does not affect the mortality of these pests, can change the conformation of complexes of DNA, RNA, and protein from the posterior region of silk gland cells of D. saccharalis, activating or repressing the expression of genes related to the defense mechanism of the insect and contributing to the selection and survival of resistant individuals.

  7. Some effects of gamma irradiation on adults males of the sugarcane borer, Diatraea saccharalis (F.)

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    Sgrillo, R.B.; Wiendl, F.M.

    1981-01-01

    With the objective of checking some effects of gamma radiation on the sugarcane borer (Diatraea saccharalis (F.)), one-day ald adult males were irradiated with doses from 0-50 krad at 5 krad intervals. The insects were submitted to gamma irradiation in a 60 Co source, with a dose rate of 350 krad/hour. No significant difference was found in male longevity between treatments. Also no significant difference as found in sexual activity, represented by number of spermatophores per female, and in fertility. Fertility and egg viability decreased significantly with the dose, the viability reaching 0 at 50 krad. Occurrence of dominant lethal mutation, induced by radiation, was noted, which resulted in the death of the embryo before larvae emergence. (Author) [pt

  8. Field-evolved resistance to Bt maize in sugarcane borer (Diatraea saccharalis) in Argentina.

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    Grimi, Damián A; Parody, Betiana; Ramos, María Laura; Machado, Marcos; Ocampo, Federico; Willse, Alan; Martinelli, Samuel; Head, Graham

    2018-04-01

    Maize technologies expressing Bacillus thuringiensis (Bt) insecticidal proteins are widely used in Argentina to control sugarcane borer (Diatraea saccharalis Fabricius). Unexpected D. saccharalis damage was observed to Bt maize events TC1507 (expressing Cry1F) and MON 89034 × MON 88017 (expressing Cry1A.105 and Cry2Ab2) in an isolated area of San Luis Province. Diatraea saccharalis larvae were sampled from MON 89034 × MON 88017 fields in the area to generate a resistant strain (RR), which was subsequently characterized in plant and diet bioassays. Survivorship of the RR strain was high on TC1507 leaf tissue, intermediate on MON 89034 × MON 88017, and low on MON 810 (expressing Cry1Ab). The RR strain had high resistance to Cry1A.105 (186.74-fold) and no resistance to Cry2Ab2 in diet bioassays. These results indicate resistance to Cry1F and Cry1A.105 (and likely cross-resistance between them) but not to Cry1Ab or Cry2Ab2. Resistance to MON 89034 × MON 88017 was functionally recessive. Reviews of grower records suggest that resistance initially evolved to Cry1F, conferring cross-resistance to Cry1A.105, with low refuge compliance as the primary cause. A mitigation plan was implemented in San Luis that included technology rotation, field monitoring, and grower education on best management practices (BMPs) including refuges. In the affected area, the resistance to Cry1F and Cry1A.105 is being managed effectively through use of MON 89034 × MON 88017 and MON 810 in combination with BMPs, and no spread of resistance to other regions has been observed. © 2017 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. © 2017 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

  9. Geographic population structure of the sugarcane borer, Diatraea saccharalis (F. (Lepidoptera: Crambidae, in the southern United States.

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    Andrea L Joyce

    Full Text Available The sugarcane borer moth, Diatraea saccharalis, is widespread throughout the Western Hemisphere, and is considered an introduced species in the southern United States. Although this moth has a wide distribution and is a pest of many crop plants including sugarcane, corn, sorghum and rice, it is considered one species. The objective was to investigate whether more than one introduction of D. saccharalis had occurred in the southern United States and whether any cryptic species were present. We field collected D. saccharalis in Texas, Louisiana and Florida in the southern United States. Two molecular markers, AFLPs and mitochondrial COI, were used to examine genetic variation among these regional populations and to compare the sequences with those available in GenBank and BOLD. We found geographic population structure in the southern United States which suggests two introductions and the presence of a previously unknown cryptic species. Management of D. saccharalis would likely benefit from further investigation of population genetics throughout the range of this species.

  10. Seleção e caracterização de estirpes de Bacillus thuringiensis eficientes contra a Diatraea saccharalis (Lepidoptera: Crambidae Selection and characterization of Bacillus thuringiensis efficient strains against Diatraea saccharalis (Lepidoptera: Crambidae

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    Cristina Lima de Macedo

    2012-12-01

    Full Text Available O objetivo deste trabalho foi selecionar e caracterizar estirpes nativas de Bacillus thuringiensis tóxicas a Diatraea saccharalis (Lepidoptera: Crambidae. Cento e seis estirpes pertencentes ao Banco de Bactérias de Invertebrados, da Embrapa Recursos Genéticos e Biotecnologia, foram testadas quanto à toxicidade a D. saccharalis, e, as mais tóxicas, caracterizadas por métodos bioquímicos e moleculares. Das 106 estirpes testadas, 16 causaram 100% de mortalidade em 24 horas. As três estirpes mais tóxicas apresentaram concentração letal média entre 8 e 43 ng cm-2. O perfil proteico das 16 estirpes mostrou a presença de proteínas de 130 e 65 kDa, e a caracterização molecular mostrou a presença dos genes tipo cry1 e cry2: cry1Aa, cry1Ab, cry1Ac e cry2Aa. A concentração letal média de esporos e cristais obtidos a partir de estirpes recombinantes, que expressavam individualmente os genes cry1Aa, cry1Ab, cry1Ac e cry2Aa, variou entre 222 e 610 ng cm-2, valores muito superiores aos das estirpes nativas mais tóxicas, que apresentavam possibilidade de expressão simultânea desses genes. Este resultado é indicativo de que há sinergia entre as toxinas. Há interação entre as toxinas de B. thuringiensis e seus receptores na broca-do-colmo da cana-de-açúcar.The objective of this work was to select and characterize native strains of Bacillus thuringiensis toxic to Diatraea saccharalis (Lepidoptera: Crambidae. A hundred-and-six strains, belonging to the bank of invertebrate bacteria (Brazil, of Embrapa Genetic Resources and Biotechnology, were tested as to their toxicity to D. saccharalis, and the most toxic ones were characterized by biochemical and molecular methods. Out of the 106 tested strains, 16 caused 100% mortality within 24 hours. The three most toxic strains showed median lethal concentrations between 8 and 43 ng cm-2. The protein profile of the 16 strains showed the presence of 130 and 65 kDa proteins, and the molecular

  11. Resistance Risk Assessment of Spodoptera frugiperda (Lepidoptera: Noctuidae) and Diatraea saccharalis (Lepidoptera: Crambidae) to Vip3Aa20 Insecticidal Protein Expressed in Corn.

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    Bernardi, Oderlei; Bernardi, Daniel; Amado, Douglas; Sousa, Renan S; Fatoretto, Julio; Medeiros, Fernanda C L; Conville, Jared; Burd, Tony; Omoto, Celso

    2015-12-01

    Transgenic Agrisure Viptera 3 corn that expresses Cry1Ab, Vip3Aa20, and EPSPS proteins and Agrisure Viptera expressing Vip3Aa20 are used for control of Spodoptera frugiperda (J.E. Smith) and Diatraea saccharalis (F.) in Brazil. To support a resistance management program, resistance risk assessment studies were conducted to characterize the dose expression of Vip3Aa20 protein and level of control against these species. The Vip3Aa20 expression in Agrisure Viptera 3 and Agrisure Viptera decreased from V6 to V10 stage of growth. However, Vip3Aa20 expression in Agrisure Viptera 3 at V6 and V10 stages was 13- and 16-fold greater than Cry1Ab, respectively. The Vip3Aa20 expression in lyophilized tissue of Agrisure Viptera 3 and Agrisure Viptera diluted 25-fold in an artificial diet caused complete larval mortality of S. frugiperda and D. saccharalis. In contrast, lyophilized tissue of Bt11 at the same dilution does not provide complete mortality of these species. Agrisure Viptera 3 and Agrisure Viptera also caused a high level of mortality against S. frugiperda and D. saccharalis. Moreover, 100% mortality was observed for S. frugiperda larvae (neonates through fifth-instar larvae) when fed in corn with the Vip trait technology. Viptera corn achieves a high level of control against S. frugiperda and D. saccharalis providing a high dose, which is an important determination to support the refuge strategy for an effective resistance management program. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  12. Influência do Fotoperíodo no Parasitismo de Trichogramma galloi Zucchi (Hymenoptera: Trichogrammatidae em Ovos de Diatraea saccharalis (Fabricius (Lepidoptera: Crambidae

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    Danilo Santana

    2013-07-01

    Abstract. Trichogramma species are an important group of parasitoids that attack especially lepidopteran eggs. The quality and performance of this natural enemy may be influenced by factors such as alternative host used in the massal rearing, and differences in the temperature and photoperiod. Based on this, this study aimed to evaluate the number of parasitized eggs, % of females that parasitized, % emergence, sex ratio and number of individuals of Trichogramma galloi Zucchi per egg of Diatraea saccharalis (Fabricius in the presence and absence of light. The experimental design was completely randomized with two treatments (light and dark and 60 replications, being each experimental unit composed by 40 eggs of D. saccharalis which were placed in a glass tube with one female of T. galloi with age of 24 hours, previously fed with honey. The experiment was carried out at 25 ± 2 °C and 70 ± 10% relative humidity. In each one of the treatments, the parasitoid was removed from the tube after 12 hours. The highest number of parasitized eggs (12.9 and % of females that parasitized (83.3% was observed during the period with light. For the emergence, sex ratio and number of individuals per egg, there was no statistical difference. The results showed that the presence of light is essential for this biological control agent.

  13. Efeito de Extratos Aquosos de Azadirachta indica na Sobrevivência de Diatraea saccharalis e do Parasitóide Cotesia flavipes

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    Fábio Mazzonetto

    2013-09-01

    Full Text Available O objetivo do presente trabalho foi avaliar o efeito de extratos aquosos de Azadirachta indica na sobrevivência de Diatraea saccharalis e do parasitóide Cotesia flavipes. Para a obtenção do extrato vegetal de A. indica, as folhas secas em estufa de circulação forçada de ar (40oC durante 48h, posteriormente trituradas em moinho de facas até obtenção do pó. Os extratos foram obtidos a partir da adição de 0,5; 1,0; 2,0; 3,0 e 5,0g do pó vegetal em 100ml de água destilada formando respectivamente as concentrações. O delineamento experimental foi inteiramente casualizado, com seis tratamentos (testemunha, 0,5%, 1%, 2%, 3% e 5% de extrato aquoso de A. indica e 40 repetições. Para a análise estatística utilizou-se o método de Kaplan-Meier e o teste de Log-Rank na obtenção e comparação das curvas de sobrevivência. Já para a análise dos dados de emergência de adultos do parasitóide C. flavipes foi utilizado o teste de Scott-Knott ao nível de 5% de probabilidade.  As lagartas D. saccharalis apresentaram um menor tempo de sobrevivência quando expostas as dietas artificiais contendo as diferentes concentrações do extrato aquoso de A. indica.  A utilização de extrato aquoso de A. indica afetou negativamente a emergência de adultos de C. flavipes, importante parasitóide de D. saccharalis, quando submetidos ao parasitismo de lagartas tratadas com extrato aquoso de folhas deste vegetal.

  14. Seleção e caracterização de estirpes de Bacillus thuringiensis eficientes contra a Diatraea saccharalis (Lepidoptera: Crambidae

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    Cristina Lima de Macedo

    2012-12-01

    Full Text Available O objetivo deste trabalho foi selecionar e caracterizar estirpes nativas de Bacillus thuringiensis tóxicas a Diatraea saccharalis (Lepidoptera: Crambidae. Cento e seis estirpes pertencentes ao Banco de Bactérias de Invertebrados, da Embrapa Recursos Genéticos e Biotecnologia, foram testadas quanto à toxicidade a D. saccharalis, e, as mais tóxicas, caracterizadas por métodos bioquímicos e moleculares. Das 106 estirpes testadas, 16 causaram 100% de mortalidade em 24 horas. As três estirpes mais tóxicas apresentaram concentração letal média entre 8 e 43 ng cm-2. O perfil proteico das 16 estirpes mostrou a presença de proteínas de 130 e 65 kDa, e a caracterização molecular mostrou a presença dos genes tipo cry1 e cry2: cry1Aa, cry1Ab, cry1Ac e cry2Aa. A concentração letal média de esporos e cristais obtidos a partir de estirpes recombinantes, que expressavam individualmente os genes cry1Aa, cry1Ab, cry1Ac e cry2Aa, variou entre 222 e 610 ng cm-2, valores muito superiores aos das estirpes nativas mais tóxicas, que apresentavam possibilidade de expressão simultânea desses genes. Este resultado é indicativo de que há sinergia entre as toxinas. Há interação entre as toxinas de B. thuringiensis e seus receptores na broca-do-colmo da cana-de-açúcar.

  15. Efeito de Beauveria bassiana (Bals. Vuill. e Metarhizium anisopliae (Metsch. Sorok. sobre características biológicas de Diatraea saccharalis F. (Lepidoptera: Crambidae = Effect of Beauveria bassiana (Bals. Vuill. and Metarhizium anisopliae (Metsch. Sorok. on Biological Characteristics of Diatraea saccharalis F. (Lepidoptera: Crambidae

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    Marco Aurélio Paes de Oliveira

    2008-04-01

    Full Text Available A broca da cana-de-açúcar Diatraea saccharalis F. é considerada uma das principais pragas nas Américas. Entre os métodos de controle, o uso de fungos entomopatogênicos tem sido amplamente recomendado no manejo das pragas da cana-de-açúcar, incluindo outras lepidobrocas. Assim sendo, este estudo investigou os efeitos de diferentes concentrações de Beauveria bassiana (Bals. Vuill. e Metarhizium anisopliae (Metsch. Sorok. sobre parâmetros biológicos da broca da cana-de-açúcar. Larvas de terceiro instar de D. saccharalis foram tratadas com os fungos usando as concentrações de 103, 104 e 105 conídios mL-1. Larvas tratadas com 105 conídios mL-1 de B. bassiana tiveram menor sobrevivência (56,6%, comparadas com lagartas não-tratadas (90%. Adultos originados de larvas tratadas colocaram menor número de ovos, com menor viabilidade, e viveram menos, comparados com adultos originados de larvas não-tratadas. Larvas tratadas com M. anisopliae na concentração de 105 conídios mL-1 e adultos originados destas larvas também exibiram redução no desempenho, comparados aos insetos não-tratados. Os resultados indicam que B. bassiana e M. anisopliae, além de patogênicos àslarvas de D. saccharalis, também interferem negativamente na sua biologia, mostrando potencial de uso contra esta praga.The sugarcane borer Diatraea saccharalis F. is considered oneof the major sugarcane pests in the American continent. Among control methods, the use of entomopathogenic fungi has been broadly recommended to manage sugarcane pests, including other sugarcane borers. Therefore, this study investigated the effects of differentconcentrations of Beauveria bassiana (Bals. Vuill and Metarhizium anisopliae (Metch. Sorok on biological characteristics of the sugarcane borer. Third-instar larvae of D. saccharalis werefungi-treated using the concentrations of 103, 104 and 105 conidia mL-1. Larvae treated with 105 conidia mL-1 of B. bassiana showed lower survival

  16. Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda

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    Lemes, Ana Rita Nunes; Davolos, Camila Chiaradia; Legori, Paula Cristina Brunini Crialesi; Fernandes, Odair Aparecido; Ferré, Juan; Lemos, Manoel Victor Franco; Desiderio, Janete Apparecida

    2014-01-01

    Second generation Bt crops (insect resistant crops carrying Bacillus thuringiensis genes) combine more than one gene that codes for insecticidal proteins in the same plant to provide better control of agricultural pests. Some of the new combinations involve co-expression of cry and vip genes. Because Cry and Vip proteins have different midgut targets and possibly different mechanisms of toxicity, it is important to evaluate possible synergistic or antagonistic interactions between these two classes of toxins. Three members of the Cry1 class of proteins and three from the Vip3A class were tested against Heliothis virescens for possible interactions. At the level of LC50, Cry1Ac was the most active protein, whereas the rest of proteins tested were similarly active. However, at the level of LC90, Cry1Aa and Cry1Ca were the least active proteins, and Cry1Ac and Vip3A proteins were not significantly different. Under the experimental conditions used in this study, we found an antagonistic effect of Cry1Ca with the three Vip3A proteins. The interaction between Cry1Ca and Vip3Aa was also tested on two other species of Lepidoptera. Whereas antagonism was observed in Spodoptera frugiperda, synergism was found in Diatraea saccharalis. In all cases, the interaction between Vip3A and Cry1 proteins was more evident at the LC90 level than at the LC50 level. The fact that the same combination of proteins may result in a synergistic or an antagonistic interaction may be an indication that there are different types of interactions within the host, depending on the insect species tested. PMID:25275646

  17. Estimación de incidencia de daños provocados por larvas de Diatraea saccharalis (Lepidoptera: Crambidae en tallos de maíz mediante evaluación de signos externos de infestación Estimating the incidence of corn stem damage produced by Diatraea saccharalis (Lepidoptera: Crambidae larva through assessment of external infestation signs

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    G. Serra

    2006-06-01

    Full Text Available Diatraea saccharalis es la plaga del cultivo de maíz de mayor impacto económico en la región pampeana de la Argentina. Las larvas ocasionan pérdidas por daños de tipo fisiológico y mecánico. Las pérdidas por daño mecánico aumentan a medida que se retrasa la fecha de cosecha, y una de las medidas culturales para prevenirlas es la cosecha anticipada. El objetivo de este trabajo fue estudiar la relación entre el número de entrenudos con galerías producidas por larvas grandes y el número de orificios por planta ocasionados por D. saccharalis. El estudio se realizó en Manfredi (Córdoba, Argentina durante la campaña agrícola 1999/2000. Se siguió un diseño experimental en parcelas divididas con cinco fechas de siembra y dos niveles de protección con insecticidas (protección total y libre infestación, como factores. Se evaluaron los ajustes a seis modelos mediante análisis de regresión lineal y no lineal. El porcentaje de plantas quebradas por debajo de la espiga varió de 3,4 a 30%. El modelo lineal explicó muy bien la relación entre el número de orificios por planta y el número de entrenudos con galerías del barrenador (R2 =0,95, y para fines predictivos resultó el más adecuado.Diatraea saccharalis is economically the most important pest to corn crops in the Pampa region in Argentina. The tunneling produced by the larvae has negative consequences due to both physiological and mechanical damage (stem falling and ear dislodging. Losses caused by mechanical injuries increase as harvest is delayed, so one of the cultural methods to prevent them is to anticipate harvest. The objective of this work was to study the relationship between the number of bored internodes and the number of holes produced by large D. saccharalis larvae in the stem. The study was perfomed in Manfredi (Córdoba, Argentina during the 1999/2000 growing season. It was carried out using a split plot design with five planting dates and protection category

  18. Effect of gamma and ultraviolet radiations in isolates of Metarhizium anisopliae (METSCH) Sorokin, 1883 and its utilization aiming Diatraea saccharalis (FABRICIUS, 1794) control

    International Nuclear Information System (INIS)

    Almeida, L.C. de.

    1983-06-01

    The effects of gamma radiation and ultraviolet in isolated of the fungus Metarhizium anisopliae (Metsch) Sorokin, and the utilization of this pathogen for the Diatrae saccharalis (Fabr.) control are studied. (L.M.J.) [pt

  19. Efeito de Beauveria bassiana (Bals. Vuill. e Metarhizium anisopliae (Metsch. Sorok. sobre características biológicas de Diatraea saccharalis F. (Lepidoptera: Crambidae - DOI: 10.4025/actascibiolsci.v30i2.3627 Effect of Beauveria bassiana (Bals. Vuill. and Metarhizium anisopliae (Metsch. Sorok. on Biological Characteristics of Diatraea saccharalis F. (Lepidoptera: Crambidae - DOI: 10.4025/actascibiolsci.v30i2.3627

    Directory of Open Access Journals (Sweden)

    Valéria Wanderley Teixeira

    2008-05-01

    Full Text Available A broca da cana-de-açúcar Diatraea saccharalis F. é considerada uma das principais pragas nas Américas. Entre os métodos de controle, o uso de fungos entomopatogênicos tem sido amplamente recomendado no manejo das pragas da cana-de-açúcar, incluindo outras lepidobrocas. Assim sendo, este estudo investigou os efeitos de diferentes concentrações de Beauveria bassiana (Bals. Vuill. e Metarhizium anisopliae (Metsch. Sorok. sobre parâmetros biológicos da broca da cana-de-açúcar. Larvas de terceiro instar de D. saccharalis foram tratadas com os fungos usando as concentrações de 103, 104 e 105 conídios mL-1. Larvas tratadas com 105 conídios mL-1 de B. bassiana tiveram menor sobrevivência (56,6%, comparadas com lagartas não-tratadas (90%. Adultos originados de larvas tratadas colocaram menor número de ovos, com menor viabilidade, e viveram menos, comparados com adultos originados de larvas não-tratadas. Larvas tratadas com M. anisopliae na concentração de 105 conídios mL-1 e adultos originados destas larvas também exibiram redução no desempenho, comparados aos insetos não-tratados. Os resultados indicam que B. bassiana e M. anisopliae, além de patogênicos às larvas de D. saccharalis, também interferem negativamente na sua biologia, mostrando potencial de uso contra esta praga.The sugarcane borer Diatraea saccharalis F. is considered one of the major sugarcane pests in the American continent. Among control methods, the use of entomopathogenic fungi has been broadly recommended to manage sugarcane pests, including other sugarcane borers. Therefore, this study investigated the effects of different concentrations of Beauveria bassiana (Bals. Vuill and Metarhizium anisopliae (Metch. Sorok on biological characteristics of the sugarcane borer. Third-instar larvae of D. saccharalis were fungi-treated using the concentrations of 103, 104 and 105 conidia mL-1. Larvae treated with 105 conidia mL-1 of B. bassiana showed lower

  20. Influência da infestação de Diatraea saccharalis (Fabr. sobre parâmetros tecnológicos da cana-de-açúcar

    Directory of Open Access Journals (Sweden)

    Leila Luci Dinardo-Miranda

    2012-01-01

    Full Text Available A broca Diatraea saccharalis é uma das pragas mais importantes da cana-de-açúcar no Brasil, razão pela qual é relevante quantificar a interferência de seu ataque nos parâmetros tecnológicos dos colmos, utilizados como matéria-prima na indústria de açúcar e etanol. Assim, o objetivo do presente trabalho foi avaliar os efeitos da intensidade de infestação sobre os parâmetros tecnológicos, entre os quais fibra na cana e pol na cana, das cultivares IAC91-1099, IACSP93-3046 e IACSP95-5000. Para tanto, os colmos coletados em campo foram agrupados de maneira a compor amostras com 0, 3, 6, 9, 12 e 15% de entrenós brocados, que foram então analisadas. Maiores ataques de broca resultaram em aumento nos teores de fibra e de açúcares redutores, e redução nos teores de pol na cana e na pureza. Na média, a curva que representa a relação entre pol na cana (y e intensidade de infestação (x, % de entrenós brocados é dada por y=16,591-0,0672x, cujo coeficiente de determinação (r² foi 0,92.

  1. El papel de la densidad y la proporción sexual de adultos en la fecundidad de Diatraea saccharalis (Lepidoptera: Crambidae en jaulas de cría masiva

    Directory of Open Access Journals (Sweden)

    Pilar MEDINA PEREYRA

    2016-01-01

    Full Text Available La cría de insectos para control biológico aumentativo requiere conocer los factores que afectan la fecundidad para optimizar la producción masiva. En ge - neral, las prácticas empleadas se basan en los resultados de experiencias exitosas. Sin embargo, en muchos casos la verificación de esas prácticas en condiciones dis - tintas a las de su origen carece de soporte cuantitativo publicado y suele pasar por alto la variación derivada del manejo de los operarios (condición estándar. El ob - jetivo de este trabajo es evaluar la fecundidad del barrenador de la caña de azúcar Diatraea saccharalis Fabricius, en función del número de adultos y de la proporción sexual (número de hembras / número de adultos en jaulas de cría, en condiciones de producción estándar. En 25 jaulas se registraron número y sexo de las mariposas y número de huevos obtenido. Los resultados mostraron que la fecundidad óptima se obtiene con valores intermedios de densidad y con proporción sexual sesgada a machos. Se sugiere que el control conjunto de densidad y proporción sexual incre - menta la eficiencia en las posturas de huevos. El presente trabajo es el primero en cuantificar ambos factores en condiciones de cría masiva y revela que las prácticas comúnmente extendidas necesitan de estudios de mayor profundidad.

  2. Environmental costs of sugarcane agroecosystems; Custos ambientais de agroecossistemas da cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Mello, Renato de

    1997-07-01

    This thesis analyzes and evaluates sugarcane agroecosystems, taking into account the environmental implications and the use of natural resources as production factors. The costs are estimated in financial and energetic measures, from the implantation of the culture to the delivery of the cane to the mill. In order to perform the basic model, a business group, that presently represents the paradigm in Brazil, was selected and from those data on, scenarios of financial and energetic costs of alternative production systems were structured. The environmental aspects were evaluated as production factors and also include in the tables. Using the costs and the energetic tables, combined with the environmental implication data of the agroecosystems, three scenarios were established. The basic scenario takes into account the commercial energy used and the environment was not considered as a part of the production. In the second scenario the environmental impacts and the use of natural resources are estimated as production factors and involve costs. The third scenario simulates a situation where the agroecosystem is planned to be sustained and the environmental damages are minimized. The results indicated higher costs for the scenarios of inserting environmental costs than for the others scenarios. They also indicated that it is possible to achieve a profitable production in a sustainable ecosystem and that the differences of the energy amount between the systems remain still small, and that the sustainable perspective will only be attractive if it were based on the social definition of higher values to the natural environment. (author)

  3. Sugarcane bioethanol and the sustainable development; Alcool combustivel derivado da cana-de-acucar e o desenvolvimento sustentavel

    Energy Technology Data Exchange (ETDEWEB)

    Leme, Rodrigo Marcelo [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Fac. de Engenharia Mecanica. Programa de Pos-graduacao em Planejamento de Sistemas Energeticos

    2004-07-01

    The use of the sugarcane bioethanol in Brazil was strongly boosted during the 1970 's and 1980 's, with the advent of the National Alcohol Programme (PROALCOOL). Following this period a decade of uncertainties came up, when the decline in the Program results were noticeable. Nowadays, it seems that new and good perspectives arise, due to the emergent external and internal markets. In this paper the evolution of the sugarcane bioethanol use in Brazil is addressed, stressing the importance and legacy of the PROALCOOL. The future perspectives of the alcohol are also discussed. (author)

  4. Quantification of trash in early sugarcane cultivars; Quantificacao do palhico em cultivares precoces de cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Pauli, Fernando A. de; Silva, Helio F. da; Tasso Junior, Luiz Carlos; Marques, Diogo; Marques, Marcos O. [Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP (Brazil). Fac. de Ciencias Agrarias e Veterinarias], E-mail: fernando_abackerli@hotmail

    2010-07-01

    The energy generated from biomass of sugarcane is on the spotlight on the world stage. The straw remaining in the soil after harvest of sugarcane, brings several attributes that qualify as plant material to be used for power cogeneration. Thus, this study aimed to quantify the straw in early cultivars of sugarcane. The experimental design was randomized blocks with 6 treatments (cultivars) and three replications. At harvest, we counted the number of stems on the meter and the weight of stems was estimated agricultural productivity. After harvesting the cane was used a rectangle of 4.5 m2, which was placed randomly within each plot and all trash contained therein was weighed. From these results was estimated the number of people benefiting the energy from this trash. The SP80-1842 had the best performance, the highest amounts of trash (31.00 t ha-1), the largest index value of straw (28.33%) and more people benefit from the energy generated from the same (26.66x10-1) showing its greater suitability for cogeneration. Cultivars CTC9 and SP91-1049 presented the results less satisfactory. (author)

  5. Bagasse production potential from late sugar cane cultivars; Potencial produtivo de bagaco por cultivares tardios de cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Tulibio F. da; Silva Neto, Helio F. da; Tasso Junior, Luiz C.; Marques, Diogo; Marques, Marcos O. [Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP (Brazil). Fac. de Ciencias Agrarias e Veterinarias], E-mail: tulibio_fernandes@yahoo.com.br

    2010-07-01

    In order to diversify energy sources, the residue of sugarcane gathers attributes that qualify its use. Therefore, this study was to evaluate the potential of bagasse production in late cultivars of sugarcane. The experimental design was a randomized block design with 6 treatments (cultivars) and 3 replications. The experiment was carried out at FCAV/UNESP Jaboticabal. To calculate the productivity, it was counted the number of stems in a row linear meter, it was obtained the weight of stems. The percentage of fiber for each cultivar was determined by a calculation of estimated production and productivity of mulch. Using these values to estimate the number of people who would benefit from the energy generated from the combustion of bagasse in a process of cogeneration power. The results were submitted to analysis of variance by F test and averages compared by Tukey test at 5% probability. Cultivars RB867515, RB72454 and CTC6 showed the best performance, indicating its greater potential for power cogeneration. Cultivars CTC IAC94-2 and 2101 were lower when considering the results obtained. (author)

  6. Effect of gamma-radiation on sugar cane spirit; Efeito da radiacao gama sobre a aguardente de cana de acucar

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Maria Djiliah Camargo Alvarenga de

    2000-07-01

    Irradiation has appeared as an alternative technique in food preservation. Besides cold decontamination irradiation can increase the quality of the food through the improvement of technological properties. For alcoholic beverages ionizing radiation has been applied to wines, whiskeys and beers in countries such as Thailand and China. In those cases, the purpose of the technique was to accelerate aging, to improve the sensory characteristics and as sterilization treatment. The aim of this work was to study the effect of gamma radiation on the quality of sugar cane spirit by gas chromatography analysis of volatile compounds and sensory analysis. The sugar cane spirit newly distilled samples and commercial samples from different states (SP, CE and RJ) were irradiated either in glass or oak cask (Quercus alba sp) in a {sup 60}Co Gammacell 220 at dose rate of 7.7 kGy/h and total doses of 0; 0.1; 2; 5 and 10 kGy. The analytical determination of esters, acetaldehyde and higher alcohols were performed in a gas chromatograph with flame ionization detector employing a Megabore CG-745 column. The alcoholic graduation was measured in a Gay-Lussac alcohometer and the pH was determined using an Analyser 300 pHmeter. The color change was measured by the absorbance at 420 nm in a Shimadzu UV 1601 spectrophotometer. The acceptance tests related to odor, taste, global impression and color were analyzed using Tukey average tests (p {<=}0,05), ANOVA and histograms of panelists' scores frequency. A correlation between acetaldeyde, esters, higher alcohols levels and radiation dose was found in the sugar cane spirit newly distilled samples irradiated in oak cask and commercial samples. An increase in methanol concentration was verified, although remaining enough below the permissible limit accepted by the Brazilian Legislation. A decrease in the alcoholic graduation and pH in the irradiated samples was observed. A slight discoloration in the irradiated samples was verified. According to ANOVA statistics for the sensory analysis, no significant difference between samples, related to odor, taste, global impression was verified. However, a significant difference related to color was observed. (author)

  7. Energetic efficiency of the use of desiccant in sugarcane; Eficiencia energetica do uso de dessecante em cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Cardoso, Gilda B.C.; Ripoli, Tomaz Caetano C.; Romanelli, Thiago L. [Escola Superior de Agricultura Luiz de Queiroz(ESALQ/USP), Piracicaba, SP (Brazil)], E-mail: gildacardoso@usp.br

    2010-07-01

    Economically, sugarcane is one of the most important crop in Brazil, showing a great perspective of expansion in the cultivated area. In the last few years the sugarcane mechanical harvested area has increased, causing a series of inconveniences for the system of harvest of the sugarcane, such as: the increase in the percentage of extraneous matter and losses of sugarcane and/or fractions in the field. Due the high value invested in the system of harvest of the sugarcane, the desiccant is one of the alternatives used to minimize such inconveniences in the pre harvest. In order to quantify the demand of energy of the system, the present work used a base of energy pointers as tool for the planning process and production of sugarcane in two systems, one with application of desiccant (T1) and the other without (T2). The energy flows of entrance (EE) and exit had been established, allowing the identification of the energy rocking (BE), that quantifies the liquid profit of energy for area. The results had shown that the application of the desiccant resulted in a bigger EE in system T1 (86601.44 MJ ha{sup -1} versus 83345.45 MJ ha{sup -1}). The BE of T2 presented superior in 0.14% in relation the T1 (2241318.57 MJ ha{sup -1} against 2238062.57 MJ ha{sup -1}), indicating that the desiccant presents greater energy consumption. (author)

  8. Characteristics and properties of sugar cane trash; Caracteristicas e propriedades do palhico de cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Innocente, Andreia F. [Universidade Estadual Paulista (UNESP), Botucatu, SP (Brazil); Saglietti, Jose R. C. [Universidade Estadual Paulista (IBB/UNESP), Botucatu, SP (Brazil). Inst. de Biociencias de Botucatu], E-mail: jroberto@ibb.unesp.br

    2010-07-01

    The sugar cane processing wastes (bagasse and trash) became an important energy source which may be used in the electrical energy co-generation. This work is aimed to determine the trash physical properties, define its energetic value and ideal combination of bagasse + trash to use in conventional boilers. The trash productivity (20 t/ha), green (14.9%) and dry (71.3%) leaves, and remaining material (8.3%) was found one day after the cane crop. The trash moisture content was measured for each component and the final average value was 28.7%. The bagasse showed a 49.81% moisture average content. The higher heating value (HHV) was found for the bagasse (19.27 MJ/kg), trash (17.90 MJ/kg) and bagasse + trash mixtures in different proportions. For the lower heating value (LHV), we observed that the released energy in the trash (12.11 MJ/kg) was higher than the one in the bagasse (8.55 MJ/kg). This result was expected due to the higher bagasse moisture content. From the analysed mixtures, the 50%-50% one had the highest LHV (10.08 MJ/kg), showing that the trash left in the field after the crop may be efficient for the energy production mixed to the bagasse in 50% proportion. (author)

  9. Phytosanitary irradiation of Diatraea saccharalis, D. grandiosella, and Eoreuma loftini (Lepidoptera: Crambinae)

    Science.gov (United States)

    Phytosanitary irradiation (PI) is increasingly being used to disinfest horticultural commodities of invasive quarantine pests. Most disinfestation is done with generic treatments, where one dose is scheduled for a group of pests and/or commodities. The current generic treatment that USDA-APHIS uses...

  10. Nitrogen fertilization and its effect on production and Diatraea saccharalis Fab. attack on sugarcane

    Directory of Open Access Journals (Sweden)

    Diego Augusto Fatecha Fois

    2017-10-01

    Full Text Available Nitrogen fertilization is essential to raise sugarcane yield in soils deficient in N. The objective of this study was to evaluate the effect of nitrogen application in production and borer attack on sugarcane. The experiment was conducted at Escobar District, State of Paraguarí, Paraguay. Three sugarcane growing seasons were evaluated during 2011-2013. The experimental design was randomized blocks with seven treatments and three repetitions, with annual application of increasing of nitrogen (0, 30, 60, 90, 120, 150 and 180 kg ha-1. At 360 days of each crop cycle, harvest was performed with the following assessments: industrial plant height, number of internodes per plant, infestation intensity index and yield. The nitrogen rate of maximum technical efficiency and maximum economic efficiency was determined. Statistical analysis of variance, means comparison and regression were performed. Nitrogen fertilization did not affect plant height and number of internodes, but increased yield and borer attack. For every kg ha-1 of nitrogen applied 0.25 t ha-1 of yield is added, and 0.27 % of infestation intensity index increase is obtained. The rate of maximum technical efficiency was 157,4 kg N ha-1 for a yield of 73,6 t ha-1, and the rate of maximum economic efficiency was 136.5 kg N ha-1 for a yield of 88,2 t ha-1.

  11. Effects of gamma radiation on the behaviour of the sugar cane borer Diatrea saccharalis (Fabr., 1794) (Lepidoptera: Pyralidae)

    International Nuclear Information System (INIS)

    Contreras Duran, J.V.

    1980-08-01

    Some effects of gamma radiation on the behaviour of Diatraea saccharalis (Fabr., 1794) (Lepidoptera:Pyralidae) were studied under controlled conditions of temperature (28 +- 2 0 C) and relative humidity (75 +- 10%). The insects were reared in an artificial diet and the adults were irradiated at 24 hour age, in each treatment, to observe the effects of the gamma radiation on the sugar cane borer, in order to provide data for future researches in the Sterile Insect Technique. Cobalt-60 was used as a source of radiation with a dose-rate of 353 krad/hour. Also an activi-meter and climatized room were used. The effects of radiation under cooling (5 +- 1 0 C) and normal conditions were determined concerning the insect activity, oviposition, competitiveness, mating and longevity. These data were obtained for both sexes and the conclusions were as follows: a) The sterilizing dosis of gamma radiation do not affect the sexual behaviour of the insect, even under cooling conditions; b) the percentages of egg unviability are directly proportional to the dosis either for the females or the males; c) D. saccharalis is active only in the scotophase and therefore it can be characterized as a nocturnal insect. (Author) [pt

  12. Mechanical behavior of cementitious composites with processed sugar cane bagasse ashes; Comportamento mecanico de cimento Portland com cinza de bagaco de cana-de-acucar processada

    Energy Technology Data Exchange (ETDEWEB)

    Bezerra, Augusto C.S.; Saraiva, Sergio L.C.; Sena, Natalia O.; Pereira, Gabriela M.; Rodrigues, Conrado S.; Ferreira, Maria C.N.F., E-mail: augustobezerra@des.cefetmg.br [Centro Federal de Educacao Tecnologica Minas Gerais (CEFET-MG), MG (Brazil); Castro, Laurenn W.A.; Silva, Marcos V.M.S. [Companhia Energetica de Minas Gerais, MG (Brazil); Gomes, Romero C. [Universidade Federal de Ouro Preto (UFOP), MG (Brazil); Aguilar, Maria T.P. [Universidade Federal de Minas Gerais (UFMG), MG (Brazil)

    2014-07-01

    Sugar cane bagasse is waste from the sugar and ethanol industry and is primarily intended for burning in boilers to generate energy. As waste from the cogeneration of energy, sugar cane bagasse ashes (SCBA) are produced with no honorable destination. This paper studies the use of SCBA to partially replace Portland cement in producing cementitious composites. The ashes were processed by reburning and grinding, and after processing were characterized by a scanning electron microscope, x-ray diffraction, laser granulometry, and x-ray fluorescence spectrometry. After characterization, cement compounds were fashioned, replacing 0, 10, 20 and 30% of the cement with SCBA. The composites were mechanically evaluated by means of compression strength tests, tensile strength tests by bending. The results proved significant, indicating the possible use of SCBA when added to the cement on manufacture. (author)

  13. Influence of calcination temperature in pozolanicity of gray sugar cane bagasse; Influencia da temperatura de calcinacao na pozolanicidade da cinza de bagaco de cana-de-acucar (CBCA))

    Energy Technology Data Exchange (ETDEWEB)

    Santos, T.A.; Argolo, R.A.; Andrade, H.M.C.; Ribeiro, D.V., E-mail: tiagoassuncao@hotmail.com [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil)

    2016-07-01

    BCA (Sugar Cane Bagasse) is burned in boilers in the process of electricity cogeneration causing the generation of CBCA (Gray Sugar Cane Bagasse), which is the final residue of sucroalcooeira industry. Currently, several studies seek alternative materials that can replace Portland cement, promoting discussions on the use of pozzolanic materials in cementitious matrices. Thus, this research seeks to analyze the pozzolanicity the CBCA, obtained by calcining the residue at different temperatures, to be determined by TG / DTG and DTA tests. For analysis of pozzolanicity these ashes were used electrical conductivity techniques, chemical titration NP EN 196-5, chapelle modified NBR 15895/2010 and the IAP method (Activity Index pozzolanic NBR:5752). The results obtained during the study demostraramm no difference between the ash calcined at temperatures of 500 ° C, 600 ° C and 700 ° C. (author)

  14. Spectral variables, growth analysis and yield of sugarcane; Variaveis espectrais e indicadores de desenvolvimento e produtividade da cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Simoes, Mauricio dos Santos [Universidade Estadual de Campinas, SP (Brazil). Faculdade de Engenharia Agricola. Programa de Pos-graduacao em Engenharia Agricola; Rocha, Jansle Vieira [Universidade Estadual de Campinas, SP (Brazil). Faculdade de Engenharia Agricola. Dept. de Planejamento e Desenvolvimento Rural Sustentavel; Lamparelli, Rubens Augusto Camargo [Universidade Estadual de Campinas, SP (Brazil). Centro de Pesquisas Meteorologicas e Climaticas Aplicadas a Agricultura (CEPAGRI)]. E-mail: rubens@cpa.unicamp.br

    2005-06-01

    Spectral information is well related with agronomic variables and can be used in crop monitoring and yield forecasting. This paper describes a multi temporal research with the sugarcane variety SP 80-1842, studying its spectral behavior using field spectroscopy and its relationship with agronomic parameters such as leaf area index (LAI), number of stalks per meter (NPM), yield (TSS) and total biomass (BMT). A commercial sugarcane field in Araras/SP/Brazil was monitored for two seasons. Radiometric data and agronomic characterization were gathered in 9 field campaigns. Spectral vegetation indices had similar patterns in both seasons and adjusted to agronomic parameters. Band 4 (B4), Simple Ratio (SR), Normalized Difference Vegetation Index (NDVI), and Soil Adjusted Vegetation Index (SAVI) increased their values until the end of the vegetative stage, around 240 days after harvest (DAC). After that stage, B4 reflectance and NDVI values began to stabilize and decrease because the crop reached ripening and senescence stages. Band 3 (B3) and RVI presented decreased values since the beginning of the cycle, followed by a stabilization stage. Later these values had a slight increase caused by the lower amount of green vegetation. Spectral variables B3, RVI, NDVI, and SAVI were highly correlated (above 0.79) with LAI, TSS, and BMT, and about 0.50 with NPM. The best regression models were verified for RVI, LAI, and NPM, which explained 0.97 of TSS variation and 0.99 of BMT variation. (author)

  15. Seasonal variation of prices of sugar cane, ethanol and electric power; Variacao estacional dos precos da cana-de-acucar, alcool combustivel e energia eletrica

    Energy Technology Data Exchange (ETDEWEB)

    Melo, Carmem Ozana de; Silva, Gerson Henrique da; Bueno, Osmar de Carvalho [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Botucatu, SP (Brazil); Esperancini, Maura Seiko Tsutsui [Universidade Estadual do Oeste do Parana (UNIOESTE), Francisco Beltrao, PR (Brazil)

    2010-07-01

    The aim of this study was to assess the seasonal price of sugar cane, fuel alcohol (hydrated and anhydrous) and electricity tariffs as a way of aiding tool for optimization of energy generation, using biomass originating from cane sugar. Using the method of moving average centered was concluded that cane and electricity rates were close to seasonal average, with low range of prices, suggesting the non-occurrence of seasonal variation in prices. Unlike the seasonal indices of ethanol showed seasonal variation of prices with greater amplitude of seasonal index. Thus, the results suggest that the utilization of by-products of sugar cane to produce electrical power points to the prospect of reducing risks associated with variations in the price of ethanol, thereby contributing to greater stability and possibility to those involved in planning alcohol sector. (author)

  16. Ethanol from sugar cane in Brazil: analysis and discussion; O etanol de cana-de-acucar no Brasil: analise e discussoes

    Energy Technology Data Exchange (ETDEWEB)

    Moraes, Albemerc Moura de; Polasse, Belisa Athayde; Simao, Nathalia Machado [Universidade Federal do ABC, Santo Andre, SP (Brazil). Curso de Pos-graduacao Stricto Sensu em Energia

    2008-07-01

    Nowadays, the intense investment in renewable energy reflects the search for alternative solutions to politician-economic and socio-environment originated by energy' source predominance of fossil origin in the global energy matrix. In this case, the ethanol from Brazil's sugar cane is more and more writing your history on the International bio fuels market. That's mainly due to Brazil's commercial maturity, agricultural, technological achieved in last decades. However, some negatives socio environments, such as deforestation, fires and precarious job conditions, among others are a discredit for a probable future hegemony of Brazilian ethanol. In this way, it is important to set guide-line discussions about this topic, analyzing and overcoming problems that avoid a better sustainable posture. (author)

  17. Gathering straw energy balance for co-generation in sugarcane mills; Balanco energetico do recolhimento da palha para cogeracao de energia em usinas de cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Veiga, Joao Paulo Soto; Bizzo, Waldir Antonio; Carvalho, Danilo Jose; Berton, Rafael Piatto [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Fac. de Engenharia Mecanica; Linero, Francisco Antonio Barba [Centro de Tecnologia Canavieira (CTC), Piracicaba, SP (Brazil)], E-mails: jpsveiga@fem.unicamp.br, bizzo@fem.unicamp.br, linero@ctc.com.br, liar@fem.unicamp.br, rpberton@fem.unicamp.br

    2012-11-01

    With the requirement and expansion of sugarcane harvest without burning the straw in the field of began to be seen as a potential fuel for co-generation sugarcane mills together bagasse. This study examined the productivity and three ways of gathering and transportation of straw in order to determine the potential energy available in biomass residues and their respective energy consumption on gathering and transport operations. To this were determined parameters for the production of waste per hectare, minimum quantity to be left in the field for maintenance of soil organic carbon and erosion reducing, the amount of straw recovered and milled at the mill, material humidity and diesel and electricity consumption of each step to obtain the final balance of energy recovered. (author)

  18. Polyphenol oxidase and peroxidase in different sugarcane cultivars, in Presidente Prudente region; Polifenoloxidases e peroxidase em diferentes variedades de cana-de-acucar na regiao de Presidente Prudente

    Energy Technology Data Exchange (ETDEWEB)

    Marques, Tadeu A.; Gomes, Danilo B.; Marques, Patricia A.A.; Alves, Vagner C. [Universidade do Oeste Paulista (UNOESTE), Presidente Prudente, SP (Brazil). Curso de Agronomia], Emails: tmarques@unoeste.br, pmarques@unoeste.br, vagner@unoeste.br

    2009-07-01

    The objective in present work was compare three sugarcane cultivars (RB 72-454, RB 86-7515, IAC 86-2480), evaluating the content of polyphenoloxidase and peroxidase. These determinations had aimed at to detect possible differences between varieties thus and being to differentiate them with regard to the products most interesting to be elaborated, ethanol production or sugar production. The varieties had presented differences of behavior for studied enzymes. The activity of polyphenoloxidase was superior the activity of peroxidase. The enzyme peroxidase was presented in bigger indices in the dry and cold periods. The enzyme polyphenoloxidase was presented well changeable, but with strong trend of bigger values in the rainy periods. It can be said that distinct periods for the best use of the varieties in the sugar production or alcohol exist. (author)

  19. Trash measurement in middle sugar cane cultivars after the harvest; Quantificacao de palhico em diferentes cultivares medios de cana-de-acucar, apos a colheita

    Energy Technology Data Exchange (ETDEWEB)

    Santin, Ivan; Silva Neto, Helio F. da; Tasso Junior, Luiz Carlos; Marques, Diogo; Marques, Marcos O. [Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP (Brazil). Fac. de Ciencias Agrarias e Veterinarias. Dept. de Tecnologia], E-mail: santinivan@hotmail.com

    2010-07-01

    The introduction of mechanized harvesting of sugarcane made it possible to cut raw cane. Thus the amount of trash has produced a significant increase, which is important in that it can be used in the production of electricity through combustion. This study aimed to quantify the productivity of trash in the semi-mechanized harvesting of different middle sugarcane cultivars. The experiment was conducted at UNESP-Jaboticabal. The experimental design was a randomized block design with six treatments (cultivars) and three replications. At harvest, we counted the number of stems in the meter and the weight of stems was estimated agricultural productivity. After harvesting the cane, was used a rectangle of 4.5 m{sup 2}, which was placed randomly within each plot and all trash contained therein was weighed. Using these results to estimate the potential number of people who would benefit from the energy from this trash. Cultivar IAC94-4004 received the largest amount of trash (44.33 t ha- 1), IP (37.66%), Pd (38.00 x10-6) and TCH (118.00 t ha-1). Cultivar IAC95- 5000 obtained the less satisfactory results, generating a small amount of trash and therefore expressing lower potential energy. (author)

  20. Preparation of membranes from cellulose obtained of sugarcane bagasse; Preparacao de membranas a partir de celulose obtida do bagaco de cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Paulo Henrique Fernandes; Cioffi, Maria Odila Hilario; Voorwald, Herman Jacobus Cornelis, E-mail: fernandes_eng@yahoo.com.b [UNESP, Guaratingueta, SP (Brazil). Fac. de Engenharia; Pinho, Maria Noberta de [Instituto Superior Tecnico de Lisboa (IST) (Portugal), Dept. de Engenharia; Silva, Maria Lucia Caetano Pinto da [Universidade de Sao Paulo (EEL/USP), Lorena, SP (Brazil). Escola de Engenharia

    2010-07-01

    In this work, cellulose obtained from sugarcane bagasse to produce both cellulose and acetylated cellulose to prepare asymmetric membranes. Membranes was procedure used a mixture of materials of DMAc/ LiCl systemic in different conditions. Cellulose and acetylated cellulose were characterized by thermogravimetric (TG), Xray diffraction (XRD) and scanning Electron Microscopy (SEM). Observed less stability thermal of acetylated cellulose when compared of cellulose. All membranes procedure were asymmetric, characterized by presence of a dense skin and porous support can be observed. SEM showed that the morphology of the superficial of membranes depends on the method preparation. (author)

  1. Quantification of energy from early cultivars of sugarcane; Quantificacao da energia a partir de cultivares precoces de cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Santin, Ivan; Silva Neto, Helio F. da; Tasso Junior, Luiz Carlos; Marques, Diogo; Marques, Marcos O. [Universidade Estadual Paulista (FCAV/UNESP), Jabotical, SP (Brazil). Fac. de Ciencias Agrarias e Veterinarias. Dept. de Tecnologia], E-mail: santinivan@hotmail.com

    2010-07-01

    The success of energy generation from biomass of sugarcane is related to the amount of bagasse produced and the improvements of the equipment involved in the thermal balance of the sugarcane industrialization process. In this sense, the objective of this study was to quantify the production and productivity of bagasse in different early cultivars of sugarcane. The experimental design was a randomized block design with 6 treatments (cultivars) and 3 replications. The experiment was carried out at FCAV/UNESP-Jaboticabal. To calculate the productivity, the number of stems was counted in a meter, the weight of stems was obtained. The percentage of fiber for each cultivar was determined by calculating the estimated production and productivity of mulch. Using these values to estimate the potential number of people who would benefit from the energy generated by the combustion of bagasse an evolving process of cogeneration power. Cultivar CTC 16 (best results), showed the highest value of TCH (100.66 t ha{sup -1}), production of bagasse (315.66 kg t {sup -1}), any product residue (32.00 t ha{sup -1}) and people who could benefit (9.33 x 106). The Cultivars SP91-1049 and IACSP93-3046 presented the results less satisfactory. (author)

  2. Economic impacts of implementing the new plants to sugar cane; Impactos economicos da implementacao das novas usinas de cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Terciote, Ricardo [Universidade Estadual de Campinas (DE/FEM/UNICAMP), SP (Brazil). Fac. de Engenharia Mecanica. Dept. de Energia

    2006-07-01

    The sugar industry goes through an important moment: invest and develop to supply future demand, mainly by fuel alcohol (around 70% of new vehicles sales are from flex fuel models - and the majority of these vehicles are supplied by alcohol). Due to this foreseeable increase of alcohol demand, sugar industry has already announced 41 new projects, which will contribute with the milling of 70 million tons of sugar cane by the conclusion of these plants. In this phase of expansion, the alcohol market must be pressured for the demand, managing the supplying of the domestic and external markets. With this scenario and the possibility of sector growth which has an important role in Brazil development, this work evaluates the economic impacts of the new plants implementation investments. (author)

  3. Technical paradigm and power cogeneration with bagasse from sugar cane in Goias, Brazil; Paradigma tecnico e cogeracao de energia com bagaco de cana-de-acucar em Goias

    Energy Technology Data Exchange (ETDEWEB)

    Alves, Josias Manoel [Centro Federal de Educacao Tecnologica de Goias, Goiania, GO (Brazil). Coord. de Saneamento Ambiental

    2006-07-01

    The article shows, through technical elements, the experience of producing electrical energy, generated in some sugar industrial plants of Goias state, by burning sugar cane husks in boilers. This production has supplied the state concessionaires, but the supply has declined in the past years, due to several issues, of economic, technical and political order. Despite of this, government has done some effort in reactivating the supply, by modifying the energy politics. (author)

  4. Cellulose acetate nanocomposite with nanocellulose obtained from bagasse of sugarcane; Nanocomposito de acetato de celulose com nanocelulose obtida a partir do bagaco de cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Frirllei Cardozo dos

    2016-07-01

    This study presents a methodology for the extraction of nanocellulose of sugarcane bagasse for use in nanocomposites with cellulose acetate (CA). The bagasse sugarcane was treated with sodium hydroxide (NaOH) and sodium hypochlorite (NaClO) to remove lignin, hemicellulose, pectin and impurities. For removal of the amorphous region of cellulose microfibrils obtained from alkali treatments were submitted to acid hydrolysis with sulfuric acid under different temperature conditions. The nanocellulose obtained through acid hydrolysis heated at 45 ° C was used for the formulation of nanocomposites by smaller dimensions presented. The films were formulated at different concentrations (1, 2, 4 and 6 wt%) by the casting technique at room temperature. Each alkaline treatment was accompanied by spectrophotometry by infrared and fluorescence analysis to confirm the removal of the amorphous fraction, micrographs carried out by Scanning Electron Microscope (SEM) to display the fiber defibration. The efficiency of acid hydrolysis was confirmed by micrographs obtained by transmission electron microscope (TEM). The crystallinity index (CI) of the nanocrystals was determined by X-ray Diffraction (XRD). The surface of the obtained films were characterized by SEM and AFM microscopy of. The results showed that the sugarcane bagasse is an excellent source for nanocellulose extraction, the amorphous fraction of the fiber can be removed with the suggested alkaline treatments, and hydrolysis with H{sub 2}SO{sub 4} was efficient both in the removal of amorphous cellulose as in reducing cellulose nanoscale with a length around 250 nm and a diameter of about 10 nm. The use of heated nanocellulose obtained through hydrolysis was selected after analysis of XRD, it was confirmed that this material had higher when compared to IC hydrolysis at room temperature. The nanocomposites showed high rigidity and brittleness with high crystallinity when compared to the pure polymer film was observed by AFM and SEM of a surface with micro pores caused by the presence of nanocellulose. One of the principal reasons for non-compatible polymer/nanocellulose can be attributed to poor adhesion of the reinforcement/matrix and the use of solvent, since the different pulp of cellulose acetate is not soluble in acetone and require a surface treatment to become more compatible. (author)

  5. Characterization of red ceramic pastes incorporated with sugarcane bagasse ash wastes; Caracterizacao de massa ceramica vermelha incorporada com residuo de cinzas de bagaco de cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Faria, K.C.P.; Gurgel, R.F.; Holanda, J.N.F., E-mail: katiacpfaria@hotmail.co, E-mail: holanda@uenf.b [Universidade Estadual do Norte Fluminense (LAMAV/UENF), Campos dos Goytacazes, RJ (Brazil). Lab. de Materiais Avancados. Grupo de Materiais Ceramicos

    2010-07-01

    The alcohol industry is one sector that stands out most in the Brazilian agribusiness. Currently there is an increasing demand for sugar and ethanol for use as fuel. The processes of manufacturing these products generate large amounts of waste, the sugarcane bagasse ash waste one of the most abundant. For its chemical and mineralogical characteristics, this waste has aroused the interest of its reuse in the field of red ceramic. This study analyzes the characteristics of a red ceramic paste incorporated with up to 20 wt.% of waste. The following characteristics were performed: chemical composition, X-ray diffraction, particle size, morphology, and Atterberg limits. The results show that the incorporation of sugarcane bagasse ash waste influences the physical-chemical and mineralogical characteristics of red ceramic paste. (author)

  6. Potential areas for the expansion of the sugar cane production; Areas potenciais para a expansao da producao de cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2009-10-15

    The evaluation of the potential areas for the expansion of the sugar cane cultivation was based on the estimation of the agricultural productivity aptitude as function of the soil and climate characteristics using maps with geo referred in scales of 1/5,000,000. Areas had been discarded which integrate of three large biomass of the country: Amazonia, Pantanal and Mata Atlantica, and all the areas with some type of restriction, such as environmental reservations, national parks, indigenous, military and urban areas. Were not considered areas with crop mechanization essential for the environmental and social sustainability with declivity more than 12%.

  7. Potential of bagasse production from middle sugarcane cultivars; Potencial de producao de bagaco por cultivares medias de cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Lebre, Antonio Carlos Pereira; Siva Neto, Helio Francisco da; Marques, Diogo; Marques, Marcos Omir; Tasso Junior, Luiz Carlos [Universidade Estadual Paulista (FCAV /UNESP), Jaboticabal, SP (Brazil). Fac. de Ciencias Agrarias e Veterinarias. Dept. de Tecnologia], E-mail: antoniocplebre8@hotmail.com

    2010-07-01

    In the search for sustainability a major concern relates to the sugarcane agroindustry waste, and their potential use as an energy source. In this sense, the objective was to identify the production and productivity of bagasse of different sugarcane cultivars, aimed at the completion of the cogeneration of electricity. The experimental design was a randomized block design with 6 treatments (cultivars) and 3 replications. The experiment was carried out at FCAV/UNESP-Jaboticabal. To calculate the productivity, the number of stems was counted in a meter, obtaining the weight of stems. The percentage of fiber for each cultivar was determined by calculating the estimated production and productivity of mulch. Using these values to estimate the potential number of people who could benefit from the electricity that would be produced by the combustion of bagasse generated within a system of cogeneration of electricity put in practice by the sugar mills and ethanol distilleries. Cultivars with the best performance IAC91-1099 and CEC 15 who obtained the highest production and productivity of mulch increases the number of people benefited. Cultivars IAC94-4004-5000 and IAC95 showed intermediate results and the cultivars SP81-3250 and RB855536 obtained less satisfactory results. (author)

  8. Analysis of electric power cogeneration using sugar cane bagasse; Uma analise da cogeracao de energia eletrica usando bagaco de cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, Anna Cristina Barbosa Dias de

    1997-07-01

    Brazil impels its economy again. A development expected in 80 and 90 years it is real. This growth demands new technologies, new researches and bases that bear that growth. Electric power is in these bases, but Brazil is not ready for that. Electric power cogeneration possibility appears, using sugar cane bagasse. Alcohol and sugar plants have already that practice working with a low generation volume. With some investment this volume can be increased, adding about 10% to national energetic matrix. The aim of this work is to present a short time alternative for national electric matrix. It shows the energetic situation of the country, some experiences already implanted in some countries around the world and some options for equipment improvement used in alcohol and sugar plants. It is shown alternatives sources of electric power generation studied on Brazil, as well as the planning of National Energetic Program of ELETROBRAS. It analyses, in details, sugar cane bagasse use, which is used in Sao Paulo plants to generate electric power. Possible systems and troubles for its implantation in sugar and alcohol plants are discussed. (author)

  9. INFESTAÇÃO DE DIATRAEA spp. EM DIFERENTES VARIEDADES DE CANA-DE-AÇÚCAR EM UNIÃO - PI

    Directory of Open Access Journals (Sweden)

    GILSON LAGES FORTES PORTELA

    2011-01-01

    Full Text Available The bit of cane sugar, Diatraea saccharalis (Fabricius, 1794, (Lepidoptera: Crambidae, is the main plague of sugar cane in Brazil. The objective of this study was to evaluate the intensity of infestation in five varieties of commercially sugar cane. The experiment was conducted at Parnaíba Valley Company (COMVAP, that belongs to the Olho D'água Group, located in the city of União - Pi (latitude 04° 51' 08" S; longitude 42° 52' 59" W. Gallo methodology was used to evaluate the the intensity of infestation. It is calculated by: II% = 100 x blocked internodes / total number of internodes. The intensity of infestation of all sorts was considered very low. The varieties SP73-2577, SP83-2847, SP81-3250, SP79-1011 and RB92-579 did not present infestation, using the no-parametric test of Kruskal - Wallis, meaningful statistic differences among them. All varieties showed similar intensity of infestation and may be recommended respecting other desired characteristics.

  10. Electricity by anaerobic digestion of vinasse from sugar cane. Technical profile, economic and environmental impacts of an option; Eletricidade por digestao anaerobia da vinhaca de cana-de-acucar. Contornos tecnicos, economicos e ambientais de uma opcao

    Energy Technology Data Exchange (ETDEWEB)

    Rego, Erik Eduardo; Moral Hernandez, Francisco del [Universidade de Sao Paulo (PIPGE/IEE/USP), SP (Brazil). Inst. de Eletrotecnica e Energia. Programa Interunidades de Pos-Graduacao em Energia

    2006-07-01

    In its first part this article brings up the growing importance of biomass in Brazilian and within the world energy scenario, especially due to the higher environmental consciousness related to the use of renewable energy sources. It also discusses about biomass sustainability and its myths. The Brazilian case shows that the usage of biomass is mixed to the use of sugar cane. The studies in this area are essentially directed to the production of ethanol and the burn of the solid waste production in steam generators. This article focuses on a specific liquid effluent product: the vinasse. The use of vinasse in agriculture as liquid fertilizer is not new. This article studies the technical, economical and environmental viability of the biogas production obtained from the anaerobic digestion of vinasse in order to generate electricity. The reactor under analysis is the UASB (Upflow Anaerobic Sludge Blanket) and a case study of a experimental process linked to alcohol production is used as a base to put side by side some points of view. Finally this article discusses how environmental regulations related to the effluent disposal (and their differences between two states in Brazil) were really trade-off issues in the decision making from the economical perspective. The combinations of the analysis (technical, economical and environmental) contributes for the global dimension of the alternative. (author)

  11. Feasibility analysis of the use of sugar cane bagasse ash as mineral addition to cementitious mortars; Analise da viabilidade de utilizacao da cinza do bagaco de cana-de-acucar como adicao mineral em argamassas cimenticias

    Energy Technology Data Exchange (ETDEWEB)

    Fazzan, J.V.; Pereira, A.M.; Moraes, M.J.B. de; Akasaki, J.L.; Sanches, A.O.; Malmonge, J. A., E-mail: jvfazzan@hotmail.com [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Ilha Solteira, SP (Brazil). Faculdade de Engenharia. Departamento de Fisica e Quimica

    2014-07-01

    Currently, Brazil is experiencing an expansion of sugarcane plantations, which makes the country the world's largest producer of sugarcane. With the bagasse generated during the process, is generated the bagasse ash cane sugar (CBC) which consists mainly of silicon dioxide (SiO{sub 2}) and presents as potential alternative raw material for the production of cement composites. In this context, the objective of this study is to evaluate the reactivity of the CBC through physical and chemical analysis, for the production of mortars. The study of the CBC was performed by means of XRF testing, XRD, SEM and ADL. Mortar specimens with different percentages of CBC in partial replacement of Portland cement, for analysis of compressive strength were also produced. Despite the mixtures with additions have lower resistance to conventional mortars, the results showed the potential of the CBC as reactive mineral addition. (author)

  12. {sup 1}H-NMR characterization of cellulose acetate obtained from sugarcane bagasse; Caracterizacao de acetato de celulose obtido a partir do bagaco de cana-de-acucar por {sup 1}H-RMN

    Energy Technology Data Exchange (ETDEWEB)

    Cerqueira, Daniel A., E-mail: daniel.cerqueira@ufba.b [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Inst. de Ciencias Ambientais e Desenvolvimento Sustentavel; Rodrigues Filho, Guimes [Universidade Federal de Uberlandia (UFU), MG (Brazil). Inst. de Quimica; Carvalho, Rui de A. [Universidade de Coimbra (Portugal). Dept. de Bioquimica; Valente, Artur J.M. [Universidade de Coimbra (Portugal). Dept. de Quimica

    2010-07-01

    Cellulose from sugarcane bagasse was used for synthesizing cellulose acetate with different degrees of substitution, which were characterized by {sup 1}H-NMR through the relationship between the peak areas of the hydrogen atoms of the acetate groups (-(C=O)OCH{sub 3}) and the peaks of the hydrogen bonded to the carbon atoms of the glycosidic rings. Suppression of some signals was carried out in order to remove the residual water resonance in the materials and those related to impurities in cellulose triacetate as well. A deconvolution method for the computation of the degree of substitution of acetylation is proposed. The degrees of substitution for the cellulose samples were 2.94 and 2.60, in good agreement with those obtained by chemical determination through an acid-base titration. (author)

  13. Characterization of cellulose acetate obtained from sugarcane bagasse by {sup 1}H-NMR; Caracterizacao de acetato de celulose obtido a partir do bagaco de cana-de-acucar por {sup 1}H-RMN

    Energy Technology Data Exchange (ETDEWEB)

    Cerqueira, Daniel A.; Rodrigues Filho, Guimes, E-mail: d.a.cerqueira@gmail.co [Universidade Federal de Uberlendia (IQ/UFU), MG (Brazil). Inst. de Quimica; Carvalho, Rui A. [Universidade de Coimbra (UC) (Portugal). Dept. de Bioquimica; Valente, Artur J.M. [Universidade de Coimbra (UC) (Portugal). Dept. de Quimica

    2009-07-01

    Cellulose from sugarcane bagasse was used for synthesizing cellulose acetate with different degrees of substitution, which were characterized by {sup 1}H-NMR through the relationship between the peak areas of the hydrogen atoms present at the acetate groups (-(C=O)OCH{sub 3} ) and the peaks of the hydrogen bonded to the carbon atoms of the glycosidic rings. Suppression was carried out in order to remove the peak of residual water in the materials and the peak related to impurities in cellulose triacetate. Degree of substitution values obtained through the resonance deconvolution were compared to those obtained by chemical determination through an acid-base titration. The determined degrees of substitution of the cellulose samples were 2.94 and 2.60. (author)

  14. Analysis of energy performance in two systems of cogeneration used in plants of sugar cane; Analise de desempenho energetico em dois sistemas de cogeracao empregados em usinas de cana de acucar

    Energy Technology Data Exchange (ETDEWEB)

    Rocha, Felipe A.A.; Rosa, Rodrigo A. [Cogeracao Sistemas de Energia Ltda., Recife, PE (Brazil)

    2004-07-01

    One of the options to overcome the current volatility in Brazil's power consumption outlook relates to the employment of other sources for power generation, namely solar energy, wind power or the use of biomass, namely the power generation through the cane bagasse. One should realize, however, that the economic accomplishment for launching a generation system should depend on the level of effectiveness of all processes and/or equipment comprising the system thereof. As far as the sugar-alcohol industry is concerned, the larger the system effectiveness is, the bigger the surplus of bagasse becomes and, hence, the better the chance of achieving financial earnings. Two generation systems shall be evaluated, aiming to thermo-dynamically identify the differences between the use of small equipment-driven turbines (like choppers, shredders and mills) and multi-stage turbo-generators, in order to replace the electric-powered drive units. Therefore, one shall follow thermodynamic-based criteria, namely the steam specific consumption, the equipment availability and effectiveness, thus allowing a comparison for each engine. Such survey shows that the effectiveness reaches nearly 68% for the turbo-generators and 43% for the small turbines. Under the economic perspective, one can find a saving of 89.500 tonnes per crop of bagasse, standing for an additional turnover of US$ 895.000,00 annually. (author)

  15. Microstructural analysis of the potential of sugarcane bagasse ash as a pozzolan material in cement composites; Analise microestrutural do potencial das cinzas de bagaco de cana-de-acucar como material pozolanico em compositos cimenticios

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, A.M.; Assuncao, C.C.; Guimaraes, L. de M.; Malmonge, J.A.; Tashima, M.M; Akasaki, J.L., E-mail: jorge.akasaki@gmail.com [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Ilha Solteira, SP (Brazil). Faculdade de Engenharia

    2016-07-01

    For civil construction, the ash obtained by burning sugarcane bagasse (SCBA) in sugar-cane industry is being treated as a pozzolan material because, in addition to containing high amounts of silicon and aluminium oxides, can promote reduction of the environmental impact caused by cement production, since this alternative material may partially replace the Portland cement.The present study evaluated the pozzolanic potential of the SCBA, from different states of Brazil (Sao Paulo (SP), Goias (GO) and Mato Grosso (MT)). The reactivity of the material was analyzed by microstructural characterization, besides the pastes production (lime / SCBA and cement / SCBA) for the analysis of the hydration products formed, which are evaluated by TG and SEM. There was a decrease in the formation of ettringite in the matrixes, inversely proportional to the amount of ash, which favored the reduction of the cracking in cementitious matrices. It has also observed that the pastes produced with the ashes from State of SP showed greater fixation of lime and, consequently, a high reactivity. (author)

  16. Biomass production by filamentous fungi in sugar cane vinasse medium supplemented with molasses; Producao de biomassa por fungos filamentosos em meio de vinhaca de cana de acucar suplementado com melaco

    Energy Technology Data Exchange (ETDEWEB)

    Ruegger, Marcelo Jose Silveira [UNESP, Rio Claro, SP (Brazil). Dept. de Ecologia; Tauk-Tornisielo, Samia Maria [UNESP, Rio Claro, SP (Brazil). Centro de Estudos Ambientais

    1996-06-01

    Three species of filamentous fungi were selected and cultivated in vinasse media with different addition of molasses. The microorganisms, previously adapted to the respective medium, were cultivated in pure and mixed cultures with constant agitation with four repetition for each sample. The biomass was separated by vacuum filtration and dried in oven until right weight, and the obtained liquid was submitted to COD analysis. The data were statistically analysed. Results are presented 21 refs., 5 figs., 2 tabs.

  17. Impact of financial environmental incentives in the potential of electric power generation on the sugar cane plants; Impacto de incentivos financeiros de natureza ambiental no potencial de geracao de eletricidade nas usinas de cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Pinto, Claudio Plaza; Walter, Arnaldo [Universidade Estadual de Campinas, SP (Brazil). Faculdade de Engenharia Mecanica. Area Interdisciplinar de Planejamento de Sistemas Energeticos]. E-mail: claudiop@fem.unicamp.br; awalter@fem.unicamp.br

    1999-07-01

    The aim of the work is to present the electric power generation from biomass and the economic potential from sugar cane plants in Brazil. Computerized electricity costs simulation are presented and several financial incentives and external market effects are considered. The results are also presented and criticized.

  18. Biomass production and biological depuration of sugar cane vinasse by mixed culture of filamentous fungi and yeasts; Producao de biomassa e depuracao biologica da vinhaca de cana-de-acucar por cultura mista de fungos filamentosos e leveduras

    Energy Technology Data Exchange (ETDEWEB)

    Ceccato, Sandra Regina

    1988-12-01

    Sugar and alcohol technology has originated wastes such as vinasse with organic load that causes pollution in Brazil. Many alternatives have been proposed to convert it into useful products such as microbial protein. The aim of this work was to select mixed cultures of filamentous fungi and yeasts with high biomass production in vinasse and to study the cultural condition optimization of the selected combination based on the protein content and the waste depuration. The growth of pure cultures along the time was also evaluated as well as the amino acid composition of the biomass obtained. (author)

  19. Production and productivity of sugar cane bagasse during 2008/2009 crop season for electrical energy conservation; Producao e produtividade de bagaco de cana-de-acucar ao longo da safra 2008/2009, visando a cogeracao de energia

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Tulibio F. da; Silva Neto, Helio F. da; Tasso Junior, Luiz Carlos; Marques, Diogo; Marques, Marcos O. [Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP (Brazil). Fac. de Ciencias Agrarias e Veterinarias], E-mail: tulibio_fernandes@yahoo.com.br

    2010-07-01

    In order to diversify sources of energy generation, Brazil has encouraged cogeneration of energy from biomass. Among the various biomasses sources, sugar cane bagasse is one that combines the best economic attributes to be due mainly to industrial production in large quantities. This study aimed to evaluate the bagasse productive potential of two varieties of sugarcane during the harvest period. The experiment was conducted at UNESP, Jaboticabal County. The experimental design was completely randomized split plot, having the two sugarcane cultivars as plots, and the split times of the 11 tests during the season and 3 replications. Were estimated production (kg tc{sup -1}) and productivity (t ha{sup -1}) residue for each cultivar in their own times. The cultivars showed differences from the average production of mulch. However, obtained similar behavior throughout the season, characterized by progressive cuts. For bagasse Productivity, the cultivars showed differences in average values and behavior throughout the season. The cultivar IACSP95-5000 had the best performance in relation to production and productivity of bagasse, can be used for cogeneration for most of the season. The cultivar RB855536 presented a lower performance, achieving low production and productivity of bagasse, indicating its lower potential in relation to energy generation. (author)

  20. Evaluation of the environmental aging and mechanical properties of the polypropylene/sugarcane bagasse composites; Avaliacao do envelhecimento ambiental e das propriedades mecanicas dos compositos de polipropileno/bagaco de cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Paiva, Rayane Lima de Moura; Mulinari, Daniella Regina [Centro Universitario de Volta Redonda (UNIFOA), Volta Redonda, RJ (Brazil)

    2013-06-15

    Polypropylene (PP) reinforced with fibers from sugarcane bagasse composites in different proportions were prepared. Also environmental aging was conducted for the composites and their mechanical properties determined. The results showed that chemical treatment caused changes in color and chemical composition of the fibers, removing impurities and amorphous constituents such as lignin and hemicellulose, techniques of FTIR, X-ray diffraction and scanning electron microscopy confirmed these data. Also, it was observed that addition of natural fiber in PP matrix provided an improvement in the mechanical properties materials. The weathering test revealed a slight mass gain after 75 days, but it was clear that the inclusion of fibers has a higher mass gain compared to pure PP. (author)

  1. Phosphorus in chronosequence of burnt sugar cane in Brazilian cerrado: humic acid analysis by {sup 31}P NMR; Fosforo em cronossequencia de cana-de-acucar queimada no cerrado goiano: analise de acidos humicos por RMN de {sup 31}P

    Energy Technology Data Exchange (ETDEWEB)

    Rossi, Celeste Q.; Pereira, Marcos G.; Garcia, Andreas C., E-mail: mgervasiopereira@gmail.com [Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropedica, RJ (Brazil). Dept. de Solos; Perin, Adriano; Gazolla, Paulo R. [Instituto Federal de Educacao, Ciencia e Tecnologia Goiano, Rio Verde, GO (Brazil); Gonzalez, Antonio P. [Universidade de Coruna, ES (Spain). Faculdad de Ciencias

    2013-10-01

    The aim of this study was to identify, with the use of {sup 31}P NMR spectroscopy, organic P species in humic acids (HA) in samples from Oxisol cultivated in chronosequence with sugar cane, pasture and Cerrado. The main forms of P-type found were orthophosphate, monoester-P (phosphate sugars) and P-diester (orthophosphate). The {sup 31}P NMR technique proved capable of identifying changes in the areas studied as a function of sugar cane burning time. In areas with 1 and 5 years of burnt cane, a decrease in recalcitrant organic P in humic acids indicated the need for use of P-humic substances for plant nutrition (author)

  2. Prospective evaluation of biorefinery routes in Brazil, from sugar cane bagasse as a basic feedstock; Avaliacao prospectiva das rotas de biorefinaria no Brasil, a partir do bagaco de cana-de-acucar como materia-prima basica

    Energy Technology Data Exchange (ETDEWEB)

    Ely, Romulo Neves

    2009-12-15

    Bio refineries have been identified either as an alternative to oil refineries or as a supplement. This work seeks to understand these plants applied to the Brazilian case, which has in the alcohol-sugar sector a large number of lignocellulose material (sugar-cane bagasse) produced in large scale as a residue of the process of sugar and alcohol production. In this case, technological routes that are able to use this product as a basic feedstock for the industrial process will be described. Therefore, a model based on a set of economical and technological variables is applied to the routes. In this model, different profiles of bio refinery plants are described and compared with different hypothetical regions, which are characterized by a combination of both access to different amounts of raw material and different types of consumers. Overall, this work describes a model of both location and competition of the main bio refinery technological routes in Brazil that have the sugar-cane bagasse as the basic raw material. (author)

  3. Energy agriculture in the Vale do Sao Francisco: electric power production from the sugar cane; Agricultura de energia no Vale do Sao Francisco: a cogeracao de energia eletrica a partir da queima do bagaco de cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Fraga Neto, Amintas Ferreira; Pinto, Adeon Cecilio [Universidade Federal do Vale do Sao Francisco (UNIVASF), Juazeiro, BA (Brazil). Curso de Engenharia de Producao], E-mail: adeon.pinto@univasf.edu.br

    2009-07-01

    The continuous increasing on energy demand has generated some preoccupation for both public and private sectors involved on search and creation of new sources of energy. Within this context, fossil and hydraulic energy sources have encountered barriers to its development. In order to solve this puzzle, a new paradigm emerges based on the use of renewable sources of energy. Among these energy sources it can be mentioned solar plants, wind mills and biomass. The option for determined energy source is related to aspects such as: technological knowledge, tradition on use and availability. In a global view, the renewable resources represent 20% of the overall energy supply. This datum shows the relevance of this topic. This paper aims to expose a theoretic discussion about the adoption of renewable energy sources in the San Francisco Valley. It also offers a diagnosis of the use of such sources in household energy. (author)

  4. Identification of two components of the female sex pheromone of the sugarcane-borer Diatraea flavipennella (Lepidoptera: Crambidae)

    Czech Academy of Sciences Publication Activity Database

    Kalinová, Blanka; do Nascimento, R. R.; Hoskovec, Michal; Mendonca, A. L.; Silva, E. L.; De Freitas, M. R. T.; Cabral Jr, C. R.; Silva, C. E.; Santana, A. E. G.; Svatoš, Aleš

    2012-01-01

    Roč. 136, č. 3 (2012), s. 203-211 ISSN 0931-2048 Institutional research plan: CEZ:AV0Z40550506 Keywords : Diatraea flavipennella * sex pheromone * GCxGC-TOFMS * GC-EAD Subject RIV: CC - Organic Chemistry Impact factor: 1.560, year: 2012

  5. Immunity of an alternative host can be overcome by higher densities of its parasitoids Palmistichus elaeisis and Trichospilus diatraeae.

    Directory of Open Access Journals (Sweden)

    Gilberto Santos Andrade

    Full Text Available Interactions of the parasitoids Palmistichus elaeisis Delvare & LaSalle and Trichospilus diatraeae Cherian & Margabandhu (Hymenoptera: Eulophidae with its alternative host Anticarsia gemmatalis (Hübner (Lepidoptera: Noctuidae affect the success or failure of the mass production of these parasitoids for use in integrated pest management programs. The aim of this study was to evaluate changes in the cellular defense and encapsulation ability of A. gemmatalis pupae against P. elaeisis or T. diatraeae in adult parasitoid densities of 1, 3, 5, 7, 9, 11 or 13 parasitoids/pupae. We evaluated the total quantity of circulating hemocytes and the encapsulation rate versus density. Increasing parasitoid density reduced the total number of hemocytes in the hemolymph and the encapsulation rate by parasitized pupae. Furthermore, densities of P. elaeisis above 5 parasitoids/pupae caused higher reduction in total hemocyte numbers. The encapsulation rate fell with increasing parasitoid density. However, parasitic invasion by both species induced generally similar responses. The reduction in defensive capacity of A. gemmatalis is related to the adjustment of the density of these parasitoids to their development in this host. Thus, the role of the density of P. elaeisis or T. diatraeae by pupa is induced suppression of cellular defense and encapsulation of the host, even without them possesses a co-evolutionary history. Furthermore, these findings can predict the success of P. elaeisis and T. diatraeae in the control of insect pests through the use of immunology as a tool for evaluation of natural enemies.

  6. Relationships between soil erosion and morphometric characteristics of its hydrographic basin in areas of sugar cane culture; Relacao entre susceptibilidade a erosao de solos e caracteristicas morfometricas de suas bacias hidrograficas em area com predominio da cultura de cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Souza e Silva, Moacir de

    1988-12-31

    This paper deals with the relationships of the quantitative aspects of soil surface detachment with the characteristics of drainage pattern and relief aspects in water heads of third-order of ramification, belonging various soil units, which are cultivated with sugar cane crops. (author). 20 figs., 48 tabs., 102 refs

  7. Dynamic of N fertilizers: urea ({sup 15} N) and aqua ammonia ({sup 15} N) incorporated to the sugar cane soil. Final report; Dinamica do N dos fertilizantes: ureia ({sup 15} N) e aquamonia ({sup 15} N) incorporados ao solo na cultura da cana-de-acucar. Relatorio final

    Energy Technology Data Exchange (ETDEWEB)

    Trivelin, P C.O. [Centro de Energia Nuclear na Agricultura (CENA), Piracicaba, SP (Brazil)

    1988-05-01

    The dynamic of N fertilizers, urea and aqua ammonia, in the soil of sugar cane crops are studied with an emphasis on the horizontal and vertical moving. The nitrogen routing from urea and aqua ammonia sources, by isotopic technique with {sup 15} N in relation to the leaching, volatilization and extraction by the cultivation and residue of N immobilized manure in the soil with sugar cane plantation is also analysed. (C.G.C.).

  8. Effect of addition of sugar cane biomass ash in properties of fresh state in cement slurries for oil wells; Efeito da adicao de cinza de biomassa de cana-de-acucar nas propriedades no estado fresco de pastas de cimento para pocos de petroleo

    Energy Technology Data Exchange (ETDEWEB)

    Galvao, Lornna L.A.; Santos, Herculana T.; Souza, Pablo Diego Pinheiro; Freitas, Julio Cezar Oliveira [Universidade Federal do Rio Grande do Norte (UFRN), RN (Brazil); Nascimento, Julio Cesar S. [Universidade Federal da Bahia (UFBA), BA (Brazil); Amorim, Natalia M.M. [Universidade Potiguar (UNP), RN (Brazil); Martinell, Antonio E. [Mcgill University (MCGILL) (Canada); Universidade Federal do Rio Grande do Norte (UFRN), RN (Brazil)

    2012-07-01

    Recent studies have shown that ashes from biomass, in particular those generated by the alcohol industry have pozzolanic activity and can replace cement in many applications, reducing the consumption of cement and, consequently, the environmental impact caused by the production of this material. The present work evaluated the behavior of ash sugarcane biomass partially replacing Portland cement in concentrations of 10, 20 and 40% BWOC in oil well slurries. The results of rheology, thickening time and stability showed that the addition of 40% of biomass ash in oil well slurries significantly improves their properties, enabling the replacement of cement by ash. (author)

  9. Glass-ceramic material of the Si-Ca-K system sintered from sugarcane bagasse ash; Material vitro-ceramico do distema SiO{sub 2}-CaO-K{sub 2}O sinterizado a partir de cinza de bagaco de cana de acucar

    Energy Technology Data Exchange (ETDEWEB)

    Teixeira, S.R.; Silva, R.A.; Santos, G.C.; Santos, G.T.A., E-mail: rainho@fct.unesp.b [Universidade Estadual Paulista (DFQB/FCT/UNESP), Presidente Prudente, SP (Brazil). Fac. de Ciencias e Tecnologia. Dept. de Fisica, Quimica e Biologia; Romero, M.; Rincon, J.Ma. [Consejo Superior de Investigaciones Cientificas (IETCC/CSIC), Madri (Spain). Inst. de Ciencias de la Construccion Eduardo Torroja; Reynoso, V.C.S. [Universidade Estadual Paulista (FEIS/UNESP), Ilha Solteira, SP (Brazil). Faculdade de Engenharia

    2009-07-01

    This study analyses the crystallization of glasses obtained from two samples of sugarcane bagasse ash - SCBA (named Cinza 07 and Cinza 08) mixed with carbonates (calcium and potassium). The glasses and their crystallization were examined using differential thermal analysis (DTA), X-ray fluorescence (XRF) and X-ray diffraction (XRD). The characterizations of the ashes show that they consist mainly of crystalline materials, predominantly quartz, with iron, potassium and aluminum oxides as minor elements. For the sample Cinza07 the DTA data presents broad and overlaid crystallization peaks, indicating crystallization of more than two different phases. The DTA results of samples with different grain-size distribution show that the crystallization peak intensities increase as the sample grain-size decreases, suggesting that surface crystallization actively participate on the mechanism of crystallization. For the sample Cinza 08 the DTA data presents two well defined peaks. In this case, the more intense peak was evaluated to obtain kinetic data (Eat= 355 kJ/mol) to the major phase (Wollastonita). (author)

  10. Effects of vinasse accumulation ponds and decantation reservoirs of water used for washing cane sugar in water resources in Dobrada municipality, Sao Paulo State, Brazil; Efeitos da estocagem de vinhaca e das aguas de lavagem da cana-de-acucar na qualidade dos recursos hidricos da regiao de Dobrada, Sao Paulo

    Energy Technology Data Exchange (ETDEWEB)

    Sabadia, Jose Antonio Beltrao; Reboucas, Aldo da Cunha [Sao Paulo Univ., SP (Brazil). Inst. de Geociencias

    1996-12-31

    This work shows the results of a monitoring carried out in an area of about 21 km{sup 2}, Dobrada Municipality, central west region of the state of Sao Paulo (Brazil), where the impacts in ground water and surface water were evaluated by the infiltration and percolation of vinasse and water used for washing cane sugar, through accumulation ponds and decantation reservoirs, respectively, in areas of Bauru Aquifer (Cretaceous). The Bauru Aquifer, free and porous, occurs in a extended area of 104.000 km{sup 2} in Sao Paulo State, as a major exploitable aquifer (more than 15.000 wells), used for domestic and industrial water supplies. For the groundwater, the following measures were found in analyses exceeding drinking water standards (WHO; CONAMA/Brazil and Sao Paulo State): manganese (0,03 to 3,5 mg/l), iron (0.45 to 34 mg/l), aluminum (1 to 52 mg/l) and phosphate (0,03 to 0.38 mg/l). For superficial water, the following measures exceeded drinking water standards: total iron (3 mg/l) and phosphate (0,035 mg/l). The main purpose of this research is to improve the field methodology to characterize the impacts of vinasse and washing water of cane sugar infiltration/percolation., detaching the relationship between ground and surface water, with the major aim to supply the legislation to protect drinking water resources. (author) 11 refs.

  11. Viability analysis of electric energy cogeneration in combined cycle with sugar-cane biomass gasification and natural gas; Analise de viabilidade da cogeracao de energia eletrica em ciclo combinado com gaseificacao de biomassa de cana-de-acucar e gas natural

    Energy Technology Data Exchange (ETDEWEB)

    Correa Neto, Vicente

    2001-03-15

    The objective of this thesis is evaluate the technical and economic viability of electric energy generation projects using as fuel the biomass produced in the sugar cane Brazilian industry, specifically the cane trash, the straw and the leaves of the plant, as complemental option to the expansion of the Brazilian electric system, hour in phase of deep modification in the institutional scenery, through the sale of electric energy for direct consumers or utilities, characterizing the business possibilities for the ethanol distilleries already integrated into the energy reality of the country. The analyzed technology is thermoelectric generation with combined cycle, operating in cogeneration, integrated to biomass gasification systems for the production of combustible gas, with and without addition of natural gas. The considered technology is known by the acronym BIG/GTCC, originated in Biomass Integrate Gasification Combined Cycle Gas Turbine. The economic analysis is made herself through a modeling and construction of economy project curves based on the prices of the electric energy, of the natural gas and in the costs of the retired biomass in an mechanized way.(author)

  12. Pathogenicity of Beauveria bassiana and production of cuticle ...

    African Journals Online (AJOL)

    aghomotsegin

    2013-11-13

    Nov 13, 2013 ... cuticle-degrading enzymes in the presence of Diatraea saccharalis cuticle .... the bioassays, each larva was confined individually in plastic containers (17 x 21 x 25 ..... nism (virus, bacteria and protozoa). Only entomo-patho-.

  13. Temperature and refrigeration time of Anticarsia gemmatalis (Lepidoptera: Noctuidae pupae affect biological parameters of Trichospilus diatraeae (Hymenoptera: Eulophidae?Temperatura e tempo de refrigeração de pupas de Anticarsia gemmatalis (Lepidoptera: Noctuidae afetam parâmetros biológicos de Trichospilus diatraeae (Hymenoptera: Eulophidae?

    Directory of Open Access Journals (Sweden)

    Patrik Luiz Pastori

    2013-09-01

    Full Text Available O desenvolvimento de parasitoides em hospedeiros conservados em baixa temperatura, sem perda da qualidade, é importante para criações massais e, por isso, a reprodução de Trichospilus diatraeae Cherian & Margabandhu (Hymenoptera: Eulophidae foi avaliada em pupas de Anticarsia gemmatalis (Hübner (Lepidoptera: Noctuidae após armazenamento a 0oC ou 5oC. No experimento um, pupas de A. gemmatalis, com até 24 horas de idade, foram armazenadas a 0oC, 5oC ou 25oC (testemunha por um, três, seis, nove ou 12 dias e expostas ao parasitismo por 10 fêmeas de T. diatraeae por 24 horas. No experimento dois, pupas de A. gemmatalis, com até 24 horas de idade foram expostas ao parasitismo por 10 fêmeas de T. diatraeae por 24 horas e, posteriormente mantidas a 25oC até o parasitoide atingir o estágio de pupa, quando foram armazenados a 0oC, 5oC ou 25oC (testemunha por um, três, seis, nove ou 12 dias. No experimento um, o parasitismo de T. diatraeae em pupas armazenadas de A. gemmatalis foi superior a 90%, exceto a 25oC por 12 dias. A emergência, o número de indivíduos e a longevidade dos descendentes machos e fêmeas da geração F1 reduziram com o armazenamento (0oC ou 5oC. No experimento dois, o armazenamento de parasitoides a 5oC por um dia aumentou a emergência, não alterou o período de duração do ciclo de vida com o número de indivíduos semelhante a testemunha. A razão sexual e a largura da cápsula cefálica de machos e de fêmeas de T. diatraeae foram semelhantes a testemunha nos experimentos. Armazenar a frio (0oC ou 5oC pupas de A. gemmatalis não parasitadas ou contendo o estágio imaturo (pupa do parasitoide no seu interior, por mais de um dia, afeta negativamente a reprodução de T. diatraeae.Development of parasitoids in hosts kept at low temperatures, without loss of quality, is important for mass rearing. Accordingly, reproduction of Trichospilus diatraeae Cherian & Margabandhu (Hymenoptera: Eulophidae was evaluated on

  14. Evaluación de la selección H, mediante hermanos completos y su síntesis, para resistencia a Diatraea spp. de dos variedades de maíz

    Directory of Open Access Journals (Sweden)

    López John Jairo

    1986-12-01

    Full Text Available El criterio de selección fue el menor número de orificios de salida del insecto, en pares de plantas, hembra y macho, éstas, últimas también auto polinizadas. En infestación natural, se estudió el efecto del sistema de selección en el daño causado por el insecto, y en los caracteres correlacionados: rendimiento, días a floración femenina, prolificidad, altura de mazorca y estatura de planta. Se determinó el avance por ciclo de selección para cada uno de estos caracteres por el método de la regresión, tomando el porcentaje relativo de cada carácter con respecto a la variedad original, para cada uno de los niveles de síntesis en las dos variedades. Se obtuvieron materiales derivados en los que aparentemente se fueron acumulando genes para otros caracteres relacionados con rendimiento.An evaluation of the H selection, through full-sib in them and their synthesis, was carry out, for resistance to Diatraea spp., in two corn varieties (ETO and DIACOL V. 351. The judgment of selection was the less number of holes from the insect exit, in pairs of plants, male and female, these last ones self pollennized. Under natural infestation, the effect of the selection system was studied in the damage caused by the insect, and in the correlate yield character, the femenine floration days, prolificity, ear corn height and plant stature. The advance was determined by the selection cycle of each one ot these characters under the regretion method, taking a relative percentage of each character in respect to the original variety, for each one of the synthesis level. Derived materials were obtained in which genes for the resistance to Diatraea spp., apparently were acumulated, and indirectly genes were qathered for other characters related to the yielding.

  15. Evaluación del daño de Diatraea spp. Lep. pyralidae y su efecto en el rendimiento de genotipos de maíz Zea mays L y sorgo Sorghum bicolor en el valle del cauca Evaluación del daño de Diatraea spp. (Lep. pyralidae y su efecto en el rendimiento de genotipos de maíz (Zea mays L. y sorgo (Sorghum bicolor en el Valle del Cauca

    Directory of Open Access Journals (Sweden)

    Arango S. Rodrigo A.

    1986-06-01

    Full Text Available Evaluation in four corn and sorghum genotipes for 1983 B and 1984 A shown that infestation (lP and infestation intensity percentages (lIP changed to a same material evaluated in as equal semester as in diverse semester. When damage and yield was related two contrasting classes was found, in one which yield decrease when affected internodes numbers increase and in other the yield increase when affected internodes number increase. Diatraea spp. Begin to cause injury is nearly to 10-15 day before blossom. Generally both sorghum and corn genotipes with greater penetration average that with greatest injury. Greater contents of cellulose, lignin and silica in stalk's cellular wall correspond to low infestation percentaje. A high positive asociation was found when was related the variables IP with IIP and panicle or ear of corn weights with grain weights.En cuatro genotipos de maíz y sorgo los porcentajes de infestación (PI y de intensidad de infestación (PII variaron para un mismo material, tanto en un mismo semestre como en semestres diferentes. Al relacionar el daño con la producción se presentaron dos situaciones contrastantes, en una de las cuales la producción disminuye al aumentar el número de entrenudos afectados y en la otra la producción aumenta con el aumento del número de entrenudos afectados. Diatraea spp. Inicia su ataque alrededor de 10 a 15 días antes de la floración. En general, los genotipos con mayor promedio de penetración presentaron mayor daño. Altos contenidos de celulosa, hemicelulosa, lignina y sílice en la pared celular del tallo correspondieron a bajos PI. Se encontró alta asociación positiva al relacionar las variables PI con P II y peso de grano con peso de mazorca o panoja.

  16. Suitability of new and old association hosts for the development of selected microgastrine parasitoids of gramineous stemborers

    NARCIS (Netherlands)

    Ngi-Song, A.J.; Overholt, W.A.; Smith, J.W.; Vinson, S.B.

    1999-01-01

    The present study examined the acceptability and suitability of Old World stemborers (Chilo partellus and C. orichalcociliellus) for the development of New World parasitoids (Apanteles deplanatus and A. minator) and New World stemborers (Diatraea saccharalis and D. grandiosella) for the development

  17. The Nearctic-Caribbean species Leptotrachelus dorsalis (Fabricius, 1801): Larval descriptions with a diagnosis of immature Ctenodactylini and natural history notes on the genus and tribe (Coleoptera: Carabidae)

    Science.gov (United States)

    Adults and larvae of Leptotrachelus dorsalis (Fabricius), live in association with grasses, the larvae in the appressed leaf axils. Both adult and larval L. dorsalis eat larvae of the Sugarcane Borer, Diatraea saccharalis (Fabricius), and perhaps other insects living in the confines of the leaf shea...

  18. Effect of selectivity of herbicides and plant growth regulators used in sugarcane crops on immature stages of Trichogramma galloi (Hymenoptera: Trichogrammatidae).

    OpenAIRE

    OLIVEIRA, H. N. de; ANTIGO, M. R.; CARVALHO, G. A.; GLAESER, D. F.

    2014-01-01

    Herbicides and plant growth regulators are often used in sugarcane management. However, the use of non-selective pesticides can cause adverse effects on the efficiency of beneficial insects in integrated pest management. Within this context, this study aimed to evaluate the effect of such products on the immature stages of the parasitoid Trichogramma galloi. Eggs of Diatraea saccharalis containing the parasitoid at the egg-larva stage and at the prepupal and pupal stages were immersed in test...

  19. Effect of selectivity of herbicides and plant growth regulators used in sugarcane crops on immature stages of Trichogramma galloi (Hymenoptera: Trichogrammatidae)

    OpenAIRE

    Oliveira, H.N.; Antigo, M.R.; Carvalho, G.A.; Glaeser, D.F.

    2014-01-01

    Herbicides and plant growth regulators are often used in sugarcane management. However, the use of non-selective pesticides can cause adverse effects on the efficiency of beneficial insects in integrated pest management. Within this context, this study aimed to evaluate the effect of such products on the immature stages of the parasitoid Trichogramma galloi. Eggs of Diatraea saccharalis containing the parasitoid at the egg-larva stage and at the prepupal and pupal stages were immersed in test...

  20. Las especies de Trichogramma de Uruguay (Hymenoptera: Trichogrammatidae Trichogramma species from Uruguay (Hymenoptera: Trichogrammatidae

    Directory of Open Access Journals (Sweden)

    César Basso

    2004-07-01

    Full Text Available Fueron recolectadas cinco especies de Trichogramma Westwood en Uruguay. La más ampliamente distribuida T. pretiosum Riley parasita Diatraea saccharalis (Fabricius, Heliothis zea (Boddie, Alabama argillacea (Hübner y Argyrotaenia sphaleropa (Meyrick. Trichogramma exiguum Pinto & Platner, a menudo confundida con T. fuentesi Torre, parasitan Bonagota cranaodes (Meyrick y A. sphaleropa. Otras dos especies T. galloi Zucchi y T. distinctum Zucchi parasita D. saccharalis. Ellas presentan una morfología relativamente similar y, de acuerdo a la información disponible, no es posible decidir si son especies próximas o sinónimas. Finalmente, T. bellaunionense Basso & Pintureau fue recolectada en Uruguay hasta el momento sólo sobre D. saccharalis. Se provee una clave ilustrada para determinar esas cinco especies. Por otra parte, se incluye una revisión del uso actual y proyectado de Trichogramma para controlar diferentes plagas en diversos cultivos en este país.Five Trichogramma Westwood species were collected in Uruguay. The widely distributed T. pretiosum Riley parasitizes Diatraea saccharalis (Fabricius, Heliothis zea (Boddie, Alabama argillacea (Hübner and Argyrotaenia sphaleropa (Meyrick. Trichogramma exiguum Pinto & Platner, often confused with T. fuentesi Torre, parasitizes Bonagota cranaodes (Meyrick and A. sphaleropa. The two species T. galloi Zucchi and T. distinctum Zucchi parasitize D. saccharalis. Their morphology is relatively close and, from the information available, it is not possible to decide if they are closely related species or synonymous species. Finally, up to now, T. bellaunionense Basso & Pintureau was only collected from Uruguay on D. saccharalis. An illustrated key is provided to identify these five species. Moreover, the present and expected use of Trichogramma to control different pests in different Uruguayan crops is reviewed.

  1. Modeling evolution of resistance of sugarcane borer (Lepidoptera: Crambidae) to transgenic Bt corn.

    Science.gov (United States)

    Kang, J; Huang, F; Onstad, D W

    2014-08-01

    Diatraea saccharalis (F.) (Lepidoptera: Crambidae) is a target pest of transgenic corn expressing Bacillus thuringiensis (Bt) protein, and the first evidence of resistance by D. saccharalis to Cry1Ab corn was detected in a field population in northeast Louisiana in 2004. We used a model of population dynamics and genetics of D. saccharalis to 1) study the effect of interfield dispersal, the first date that larvae enter diapause for overwintering, toxin mortality, the proportion of non-Bt corn in the corn patch, and the area of a crop patch on Bt resistance evolution; and 2) to identify gaps in empirical knowledge for managing D. saccharalis resistance to Bt corn. Increasing, the proportion of corn refuge did not always improve the durability of Bt corn if the landscape also contained sugarcane, sorghum, or rice. In the landscape, which consisted of 90% corn area, 5% sorghum area, and 5% rice area, the durability of single-protein Bt corn was 40 yr when the proportion of corn refuge was 0.2 but 16 yr when the proportion of corn refuge was 0.5. The Bt resistance evolution was sensitive to a change (from Julian date 260 to 272) in the first date larvae enter diapause for overwintering and moth movement. In the landscapes with Bt corn, non-Bt corn, sugarcane, sorghum, and rice, the evolution of Bt resistance accelerated when larvae entered diapause for overwintering early. Intermediate rates of moth movement delayed evolution of resistance more than either extremely low or high rates. This study suggested that heterogeneity in the agrolandscapes may complicate the strategy for managing Bt resistance in D. saccharalis, and designing a Bt resistance management strategy for D. saccharalis is challenging because of a lack of empirical data about overwintering and moth movement.

  2. Influence of irrigation on the attack from some insects to sugarcane in the central zone of Cuba

    Directory of Open Access Journals (Sweden)

    Carlos Alberto Hernández Medina

    2014-07-01

    Full Text Available It was studied, in several experiments with different irrigation levels, by graveness and by aspersion,the incidence of Diatraea saccharalis (Fab., Elasmopalpus lignosellus . Zeller. and Saccharosydne saccharivora. Westwood. on sugarcane. Damage caused by borer increased in varieties Ja60-5, B72-74, C1051-73 and C266-70 with application irrigation by graveness and in the variety C 87-51 with irrigation by aspersion with machine of central pivot. Affectations of antillean grasshopper had similar answer to irrigation. However irrigation by furrows caused significant decrease in attack of ratoons smaller borer to the three evaluated varieties.

  3. Differential expression of the pr1A gene in Metarhizium anisopliae and Metarhizium acridum across different culture conditions and during pathogenesis

    Directory of Open Access Journals (Sweden)

    Mariele Porto Carneiro Leão

    2015-03-01

    Full Text Available The entomopathogenic fungi of the genus Metarhizium have several subtilisin-like proteases that are involved in pathogenesis and these have been used to investigate genes that are differentially expressed in response to different growth conditions. The identification and characterization of these proteases can provide insight into how the fungus is capable of infecting a wide variety of insects and adapt to different substrates. In addition, the pr1A gene has been used for the genetic improvement of strains used in pest control. In this study we used quantitative RT-PCR to assess the relative expression levels of the pr1A gene in M. anisopliae and M. acridum during growth in different culture conditions and during infection of the sugar cane borer, Diatraea saccharalis Fabricius. We also carried out a pathogenicity test to assess the virulence of both species against D. saccharalis and correlated the results with the pattern of pr1A gene expression. This analysis revealed that, in both species, the pr1A gene was differentially expressed under the growth conditions studied and during the pathogenic process. M. anisopliae showed higher expression of pr1A in all conditions examined, when compared to M. acridum. Furthermore, M. anisopliae showed a greater potential to control D. saccharalis. Taken together, our results suggest that these species have developed different strategies to adapt to different growing conditions.

  4. Effects of adult feeding on the reproduction and longevity of Noctuidae, Crambidae, Tortricidae and Elachistidae species

    International Nuclear Information System (INIS)

    Milano, Patricia; Berti Filho, Evoneo; Parra, Jose R. P.; Consoli, Fernando L.; Oda, Melissa L.

    2010-01-01

    This research evaluates the effect of the adult diet on the reproduction of Anticarsia gemmatalis Hubner, Heliothis virescens (Fabr.), Spodoptera frugiperda (J. E. Smith) (Noctuidae), Diatraea saccharalis (Fabr.) (Crambidae), Gymnandrosoma aurantianum Lima (Tortricidae) and Stenoma catenifer Walsingham (Elachistidae). Adults of all species were fed either water or a 10% honey solution. The egg viability for the 1 st and 2 n d egg masses, adult fecundity, longevity, number of mating and the ovigeny index (OI) (degree of ovarian maturation) were evaluated. Fecundity of A. gemmatalis and H. virescens was drastically reduced when females were fed only on water. Egg viability from both 1 st and 2 nd egg masses was variable between treatments. Females of A. gemmatalis, H. virescens and S. frugiperda, and males of some species had a reduced longevity when fed only on water. The number of matings was higher for A. gemmatalis and D. saccharalis when fed on water only. The OI was < 1.0 for all species evaluated indicating that all females may develop new oocytes as they age. Based on the OI and the reduced fecundity of A. gemmatalis and H. virescens, one observes that adult feeding is important for the reproduction of both species, and the IO is not a good parameter to indicate such condition. Spodoptera frugiperda, G. aurantianum, D. saccharalis and S. catenifer do not require any source of carbohydrates as adults to sustain their reproduction. (author)

  5. Effects of adult feeding on the reproduction and longevity of Noctuidae, Crambidae, Tortricidae and Elachistidae species; Efeito da alimentacao da fase adulta na reproducao e longevidade de especies de Noctuidae, Crambidae, Tortricidae e Elachistidae

    Energy Technology Data Exchange (ETDEWEB)

    Milano, Patricia; Berti Filho, Evoneo; Parra, Jose R. P.; Consoli, Fernando L., E-mail: patmilano@gmail.co, E-mail: eberti@esalq.usp.b, E-mail: jrpparra@esalq.usp.b, E-mail: fconsoli@esalq.usp.b [Escola Superior de Agricultura Luiz de Queiroz. (ESALQ/USP), Piracicaba, SP (Brazil). Dept. de Entomologia e Acarologia; Oda, Melissa L., E-mail: melissa.oda@gmail.co [Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP), Piracicaba, SP (Brazil). Dept. de Ciencias Florestais

    2010-04-15

    This research evaluates the effect of the adult diet on the reproduction of Anticarsia gemmatalis Hubner, Heliothis virescens (Fabr.), Spodoptera frugiperda (J. E. Smith) (Noctuidae), Diatraea saccharalis (Fabr.) (Crambidae), Gymnandrosoma aurantianum Lima (Tortricidae) and Stenoma catenifer Walsingham (Elachistidae). Adults of all species were fed either water or a 10% honey solution. The egg viability for the 1{sup st} and 2{sup n}d egg masses, adult fecundity, longevity, number of mating and the ovigeny index (OI) (degree of ovarian maturation) were evaluated. Fecundity of A. gemmatalis and H. virescens was drastically reduced when females were fed only on water. Egg viability from both 1{sup st} and 2{sup nd} egg masses was variable between treatments. Females of A. gemmatalis, H. virescens and S. frugiperda, and males of some species had a reduced longevity when fed only on water. The number of matings was higher for A. gemmatalis and D. saccharalis when fed on water only. The OI was < 1.0 for all species evaluated indicating that all females may develop new oocytes as they age. Based on the OI and the reduced fecundity of A. gemmatalis and H. virescens, one observes that adult feeding is important for the reproduction of both species, and the IO is not a good parameter to indicate such condition. Spodoptera frugiperda, G. aurantianum, D. saccharalis and S. catenifer do not require any source of carbohydrates as adults to sustain their reproduction. (author)

  6. Several Cultures project

    International Nuclear Information System (INIS)

    Anon.

    Researches carried out in the 'Many Culture Project' of the Agricultural Nuclear Energy Center, Piracicaba, Sao Paulo State, Brazil, are described. Such researches comprise: genetic improvement of wheat; genetic improvement of rice; radioentomology of fruit flies (Ceratitis capitata); soil/sugar-cane relation; biological fixation of nitrogen by sugar-cane; radioentomology of the sugar-cane borer (Diatraea saccharalis); sugar-cane manuring; electron microscopy of B. mori and A. gemmatalis polyhedrosis; electron microscopy of some varieties of coffee-plant attacked with blight; nitrogen fixation in corn; confirmation of the extent of the concept of the value 'A'; behavior of the 14 C - and 35 S soil organic matter; fractionation of organic matter from the main types of Brazilian soils and measurement of the 14 C/ 12 C specific radioactivity; study of the radicular system of some cultures; influence of organic matter on the alteration of soils located in different Brazilian climatic zones. (M.A.) [pt

  7. Biological aspects of Eriopis connexa (Germar (Coleoptera: Coccinellidae fed on different insect pests of maize (Zea mays L. and sorghum [Sorghum bicolor L. (Moench.

    Directory of Open Access Journals (Sweden)

    RB Silva

    Full Text Available Eriopis connexa (Germar (Coleoptera: Coccinellidae occurs in several countries of South America and its mass rearing is important for biological control programmes. This work evaluated biological aspects of E. connexa larva fed on eggs of Anagasta kuehniella (Zeller (Lepidoptera: Pyralidae and Spodoptera frugiperda (J. E. Smith (Lepidoptera: Noctuidae frozen for one day, fresh eggs of Diatraea saccharalis (Fabricius (Lepidoptera: Pyralidae, S. frugiperda newly-hatched caterpillars, nymphs of Rhopalosiphum maidis (Fitch and Schizaphis graminum (Rondani (Hemiptera: Aphididae. Duration of larva, pupa and larva to adult stages differed among prey offered, whereas the prepupa stage was similar. Larva, pupa, prepupa and larva to adult viabilities were equal or major of 87.5% in all prey, except for larva fed on newly-hatched larvae of S. frugiperda. Eriopis connexa has good adaptation to different prey corroborating its polyphagous feeding habit, which evidences the potential of this natural enemy for controlling corn and sorghum pests.

  8. Effect of host and food availability on the biological characteristics of Trichogramma galloi Zucchi (Hymenoptera, Trichogrammatidae)

    International Nuclear Information System (INIS)

    Pratissoli, Dirceu; Oliveira, Harley N. de; Oliveira, Regiane C. de; Zago, Hugo B.; Vieira, Stella M.J.

    2004-01-01

    Effect of host and food availability on the biological characteristics of Trichogramma galloi Zucchi (Hymenoptera, Trichogrammatidae). Biological characteristics of Trichogramma galloi Zucchi, 1988 were evaluated in laboratory where these parasitoids were reared on eggs of Diatraea saccharalis (Lepidoptera, Pyralidae) with or without honey, and exposed to eggs of the host after 0, 6, 12, 24, 36, 48, 60, 72 and 84 hours of emergence. The parasitism rate and viability showed higher for individuals that received food. The sex ratio was not influenced by food. The number of individuals per egg only showed difference for those adults that did not receive food and stayed six hours without the host eggs. Checking the effect of the availability of eggs, only the sex ratio, with or without honey, did not show differences. The results show that T. galloi needs a carbohydrate supply and the time can influence the reproductive capacity. (author)

  9. Effect of host and food availability on the biological characteristics of Trichogramma galloi Zucchi (Hymenoptera, Trichogrammatidae); Efeito da disponibilidade de hospedeiro e de alimento nas caracteristicas biologicas de Trichogramma galloi Zucchi (Hymenoptera, Trichogrammatidae)

    Energy Technology Data Exchange (ETDEWEB)

    Pratissoli, Dirceu; Oliveira, Harley N. de; Oliveira, Regiane C. de; Zago, Hugo B. [Universidade Federal do Espirito Santo (UFES), Alegre, ES (Brazil). Centro de Ciencias Agrarias], e-mail: dirceu@npd.ufes.br, e-mail: hnoliveira@insecta.ufv.br; Vieira, Stella M.J. [Universidade Federal de Vicosa (UFV), MG (Brazil). Dept. de Engenharia Agricola

    2004-03-15

    Effect of host and food availability on the biological characteristics of Trichogramma galloi Zucchi (Hymenoptera, Trichogrammatidae). Biological characteristics of Trichogramma galloi Zucchi, 1988 were evaluated in laboratory where these parasitoids were reared on eggs of Diatraea saccharalis (Lepidoptera, Pyralidae) with or without honey, and exposed to eggs of the host after 0, 6, 12, 24, 36, 48, 60, 72 and 84 hours of emergence. The parasitism rate and viability showed higher for individuals that received food. The sex ratio was not influenced by food. The number of individuals per egg only showed difference for those adults that did not receive food and stayed six hours without the host eggs. Checking the effect of the availability of eggs, only the sex ratio, with or without honey, did not show differences. The results show that T. galloi needs a carbohydrate supply and the time can influence the reproductive capacity. (author)

  10. Desenvolvimento da broca-das-cucurbitáceas em diferentes tipos de substratos alimentares Development of melonworm on different feeding substrates

    Directory of Open Access Journals (Sweden)

    Dirceu Pratissoli

    2007-12-01

    Full Text Available Aspectos biológicos de Diaphania nitidalis foram estudados sob dietas natural e artificial em condições de laboratório, à temperatura de 25±1ºC, umidade relativa de 70±10% e fotofase de 14 horas. Como dieta natural foi utilizada abóbora 'Jacaré', pepino 'Japonês' e abobrinha 'Caserta' e, como artificial, a dieta utilizada em criações de Diatraea saccharalis, broca da cana-de-açúcar. As lagartas de D. nitidalis foram inoculadas nos diferentes tipos de substratos e criadas por todo o ciclo. Ocorreu diferença significativa entre a maioria dos parâmetros testados dentro dos tratamentos, sendo as dietas à base de abóbora e artificial as que proporcionaram maior potencial biótico para criação de D. nitidalis. Pela facilidade de aquisição dos ingredientes e manipulação dos insetos, a dieta artificial se torna mais eficaz para criação massal dessa espécie em laboratório.The aim of this research was to evaluate the biological behaviour of melonworm at different natural and artificial diet under laboratory conditions (25ºC, 70% RH, 14 h photofase. Squash 'Jacaré', cucumber 'Japonês' and zucchini 'Caserta' were used as natural substrates compared to na artificial diet developed for the sugarcane borer (Diatraea saccharalis. The caterpillars were inoculated in each substrate and reared for a full cycle. The biological parameters were different among treatments. An artificial diet and squash cv. Jacaré substrate showed higher performance for the multiplication of D. nitidalis. The artificial diet is recommended because it is easy to obtain the ingredients and nultiply this insect under laboratory conditions.

  11. Efeito da disponibilidade de hospedeiro e de alimento nas características biológicas de Trichogramma galloi Zucchi (Hymenoptera, Trichogrammatidae Efect of host and food availability on the biological characteristics of Trichogramma galloi Zucchi (Hymenoptera, Trichogrammatidae

    Directory of Open Access Journals (Sweden)

    Dirceu Pratissoli

    2004-03-01

    Full Text Available Características biológicas de Trichogramma galloi Zucchi, 1988 foram avaliadas em laboratório onde esses parasitóides foram criados com ovos de Diatraea saccharalis (Lepidoptera, Pyralidae, com ou sem mel, e expostos a ovos do hospedeiro após 0, 6, 12, 24, 36, 48, 60, 72 e 84 horas da emergência. As taxas de parasitismo e de viabilidade mostraram-se elevadas para indivíduos que receberam alimento. A razão sexual não foi influenciada pelo alimento. O número de indivíduos por ovo somente mostrou diferença para aqueles adultos que não receberam alimento e permaneceram 6 horas sem ovos do hospedeiro. Conferindo o efeito da disponibilidade de ovos, somente a razão sexual, com ou sem mel, não mostrou diferenças. Os resultados mostram que T. galloi necessita de um suprimento de carboidratos e que o tempo pode influenciar a capacidade reprodutiva.Biological characteristics of Trichogramma galloi Zucchi, 1988 were evaluated in laboratory where these parasitoids were reared on eggs of Diatraea saccharalis (Lepidoptera, Pyralidae with or without honey, and exposed to eggs of the host after 0, 6, 12, 24, 36, 48, 60, 72 and 84 hours of emergence. The parasitism rate and viability showed higher for individuals that received food. The sex ratio was not influenced by food. The number of individuals per egg only showed difference for those adults that did not receive food and stayed six hours without the host eggs. Checking the effect of the availability of eggs, only the sex ratio, with or without honey, did not show differences. The results show that T. galloi needs a carbohydrate supply and the time can influence the reproductive capacity.

  12. Biologia do ectoparasitóide Bracon hebetor Say, 1857 (Hymenoptera: Braconidae em sete espécies de lepidópteros Biology of the ectoparasitoid Bracon hebetor Say, 1857 (Hymenoptera: Braconidae on seven lepidopteran species

    Directory of Open Access Journals (Sweden)

    Sandra Regina Magro

    2001-12-01

    Full Text Available Bracon hebetor Say, 1857 é um ectoparasitóide larval de várias espécies de piralídeos que atacam grãos armazenados, sendo considerado um agente potencial de controle biológico dessas pragas. Assim, a biologia de B. hebetor foi estudada em condições controladas de temperatura (25 ± 2°C, umidade relativa (60 ± 10% e fotoperíodo (fotofase de 14 horas, sobre sete espécies de lepidópteros, Anagasta kuehniella (Zeller, Corcyra cephalonica Stainton e Sitotroga cerealella (Olivier (hospedeiros naturais e Diatraea saccharalis (Fabricius, Heliothis virescens (Fabricius, Anticarsia gemmatalis Hübner, Spodoptera frugiperda (J.E. Smith (insetos criados rotineiramente no laboratório de Biologia de Insetos da ESALQ/USP, para determinar o melhor hospedeiro para sua criação em laboratório. A duração e a viabilidade do ciclo biológico (ovo-adulto foram afetadas, dependendo do hospedeiro utilizado. A. kuehniella e C. cephalonica foram hospedeiros semelhantes para a criação de B. hebetor, produzindo insetos com duração de ciclo de 12,8 dias, viabilidade em torno de 90% e ótima capacidade de paralisação e parasitismo. D. saccharalis foi o único hospedeiro não natural que proporcionou resultados semelhantes àqueles obtidos sobre as três traças hospedeiras naturais. Quanto à agressividade, D. saccharalis produziu insetos com capacidade de paralisação e parasitismo semelhantes àqueles criados sobre as traças S. cerealella e C. cephalonica.Bracon hebetor Say, 1857 is a larval ectoparasitoid of several species of pyralids which attack stored grains, therefore a potential biological control agent of these pests. To determine the best host for laboratory rearing, the biology of B. hebetor was studied under controlled temperature conditions (25 ± 2°C, relative humidity (60 ± 10% and photoperiod (14-hour photophase on seven lepidopteran larvae: natural hosts Anagasta kuehniella (Zeller, Corcyra cephalonica Stainton and Sitotroga

  13. ¿Multiplicar Tetrastichus howardi (Hymenoptera: Eulophidae en la oruga de la seda afecta su biología?

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    Nahara Gabriela Piñeyro

    2016-01-01

    Full Text Available La multiplicación sucesiva de parasitoides en hospederos alternativos, puede afectar las características biológicas y comprometer los sistemas de cría masiva de estos. El objetivo de este trabajo fue evaluar la multiplicación de Tetrastichus howardi (Olliff, 1893 (Hymenoptera: Eulophidae en pupas del hospedero alternativo Bombyx mori (Linnaeus, 1758 (Lepidoptera: Bombycidae, durante tres generaciones. Y si eso afecta su desempeño reproductivo, cuando se cría, posteriormente, en pupas del hospedero natural Diatraea saccharalis (Fabricius, 1794 (Lepidoptera: Crambidae. Las informaciones obtenidas en este estudio pueden mejorar y facilitar el control biológico de plagas, principalmente, con la utilización de parasitoides, debido a que T. howardi parasita y se desarrolla en pupas de B. mori. De forma general, la multiplicación de T. howardi durante tres generaciones en pupas del hospedero alternativo B. mori no compromete las características biológicas de este parasitoide cuando se multiplica en su hospedero natural D. saccharalis a los efectos de su utilización en programas de control biológico.

  14. Selected Abiotic and Biotic Environmental Stress Factors Affecting Two Economically Important Sugarcane Stalk Boring Pests in the United States

    Directory of Open Access Journals (Sweden)

    Allan T. Showler

    2016-02-01

    Full Text Available Sugarcane, Saccharum spp., in the United States is attacked by a number of different arthropod pests. The most serious among those pests are two stalk boring moths in the Family Crambidae: the sugarcane borer, Diatraea saccharalis (F., and the Mexican rice borer, Eoreuma loftini (Dyar. The two species are affected by abiotic and biotic environmental stress factors. Water deficit and excessive soil nitrogen alter physical and physiochemical aspects of the sugarcane plant that make the crop increasingly vulnerable to E. loftini. Weed growth can be competitive with sugarcane but it also supports enhanced abundances and diversity of natural enemies that can suppress infestations of D. saccharalis. In an instance where the stalk borer is considered a stress factor, proximity of vulnerable crops to sugarcane can influence levels of E. loftini infestation of sugarcane. The adverse effects of each stress factor, in terms of stalk borer attack, can be reduced by adopting appropriate cultural practices, such as adequate irrigation, judicious use of nitrogen fertilizer, using noncompetitive weed growth, and not planting vulnerable crops near sugarcane fields. Understanding the relationships between stress factors and crop pests can provide valuable insights for plant breeders and tools for incorporation into integrated pest management strategies.

  15. Population dynamics of caterpillars on three cover crops before sowing cotton in Mato Grosso (Brazil).

    Science.gov (United States)

    Silvie, P J; Menzel, C A; Mello, A; Coelho, A G

    2010-01-01

    Direct seeding mulch-based cropping systems under a preliminary cover crop such as millet are common in some areas of Brazil. Lepidopteran pests that damage cotton, soybean and maize crops can proliferate on cover crops, so preventive chemical treatments are necessary. Very little data is available on these pests on cover crops. This paper presents the dynamics of Spodoptera frugiperda, S. eridania, Mocis latipes and Diatraea saccharalis caterpillars monitored at Primavera do Leste, Mato Grosso state (Brazil) during the of 2005/2006 and 2006/2007 cropping seasons on four cover crops, i.e. finger millet (Eleusine coracana), pearl millet (Pennisetum glaucum), sorghum (Sorghum bicolor) and ruzigrass (Brachiaria ruziziensis). The pests were visually counted on plants within a 1 m2 transect (wooden frame). Caterpillars were reared to facilitate identification of collected species and parasitoids. Many S. frugiperda caterpillars were observed on millet in 2005, with a maximum of 37 caterpillars/m2. On sorghum, we found 30 caterpillars/m2, or 0.83 caterpillars/plant. The Diatraea borer attacked sorghum later than the other pests. M. latipes was also observed on millet. The millet cover crop had to be dried for at least 1 month before direct drilling the main cotton crop in order to impede S. frugiperda infestations on cotton plantlets, thus avoiding the need for substantial resowing. The comparative methodological aspects are discussed.

  16. Management of Field-Evolved Resistance to Bt Maize in Argentina: A Multi-Institutional Approach

    Science.gov (United States)

    Signorini, Ana M.; Abratti, Gustavo; Grimi, Damián; Machado, Marcos; Bunge, Florencia F.; Parody, Betiana; Ramos, Laura; Cortese, Pablo; Vesprini, Facundo; Whelan, Agustina; Araujo, Mónica P.; Podworny, Mariano; Cadile, Alejandro; Malacarne, María F.

    2018-01-01

    Evolution of resistance to control measures in insect populations is a natural process, and management practices are intended to delay or mitigate resistance when it occurs. During the 2012/13 season the first reports of unexpected damage by Diatraea saccharalis on some Bt maize hybrids occurred in the northeast of San Luis province, Argentina. The affected Bt technologies were Herculex I® (HX-TC1507) and VT3PRO® (MON 89034 × MON 88017*). Event TC1507 expresses Cry1F and event MON 89034 expresses Cry1A.105 and Cry2Ab2, whichr are all Bt proteins with activity against the lepidopterans D. saccharalis and Spodoptera frugiperda (MON 88017 expresses the protein Cry3Bb1 for control of coleopteran insects and the enzyme CP4EPSPS for glyphosate tolerance). The affected area is an isolated region surrounded by sierra systems to the northeast and west, with a hot semi-arid climate, long frost-free period, warm winters, hot dry summers, and woody shrubs as native flora. To manage and mitigate the development of resistance, joint actions were taken by the industry, growers and Governmental Agencies. Hybrids expressing Vip3A protein (event MIR162) and/or Cry1Ab protein (events MON 810 and Bt11) as single or stacked events are used in early plantings to control the first generations of D. saccharalis, and in later plantings date's technologies with good control of S. frugiperda. A commitment was made to plant the refuge, and pest damage is monitored. As a result, maize production in the area is sustainable and profitable with yields above the average. PMID:29888224

  17. The sugarcane defense protein SUGARWIN2 causes cell death in Colletotrichum falcatum but not in non-pathogenic fungi.

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    Flávia P Franco

    Full Text Available Plants respond to pathogens and insect attacks by inducing and accumulating a large set of defense-related proteins. Two homologues of a barley wound-inducible protein (BARWIN have been characterized in sugarcane, SUGARWIN1 and SUGARWIN2 (sugarcane wound-inducible proteins. Induction of SUGARWINs occurs in response to Diatraea saccharalis damage but not to pathogen infection. In addition, the protein itself does not show any effect on insect development; instead, it has antimicrobial activities toward Fusarium verticillioides, an opportunistic fungus that usually occurs after D. saccharalis borer attacks on sugarcane. In this study, we sought to evaluate the specificity of SUGARWIN2 to better understand its mechanism of action against phytopathogens and the associations between fungi and insects that affect plants. We used Colletotrichum falcatum, a fungus that causes red rot disease in sugarcane fields infested by D. saccharalis, and Ceratocystis paradoxa, which causes pineapple disease in sugarcane. We also tested whether SUGARWIN2 is able to cause cell death in Aspergillus nidulans, a fungus that does not infect sugarcane, and in the model yeast Saccharomyces cerevisiae, which is used for bioethanol production. Recombinant SUGARWIN2 altered C. falcatum morphology by increasing vacuolization, points of fractures and a leak of intracellular material, leading to germling apoptosis. In C. paradoxa, SUGARWIN2 showed increased vacuolization in hyphae but did not kill the fungi. Neither the non-pathogenic fungus A. nidulans nor the yeast S. cerevisiae was affected by recombinant SUGARWIN2, suggesting that the protein is specific to sugarcane opportunistic fungal pathogens.

  18. Use of P-32 in Diatraea saccharallis (Fabricius, 1794) (Lepidoptera: Pyralidae) predator's studies

    International Nuclear Information System (INIS)

    Souza-Silva, C.R.; Pacheco, J.M.; Sgrillo, R.B.; Oliveira, A.R.

    1992-01-01

    Eggs and larvae of D. Saccharallis were labelled with P-32 and spread in the sugar cane fields in order to study its predators. Results showed a restricted number of predatory species. Ants were the main predators of larvae and earwigs were the unique eggs predator. (author)

  19. Pressão de aplicação com pulverizador de barra e eficiência de bioinseticidas fúngicos comerciais Application pressure in boom sprayer and efficiency of commercial fungal bioinsecticides

    Directory of Open Access Journals (Sweden)

    Cesar de Oliveira Guimarães

    2004-12-01

    Full Text Available Bioinseticidas fúngicos são aplicados com pulverizadores convencionais e algumas características destes equipamentos podem não ser adequadas e afetar a eficiência dos bioinseticidas. O objetivo deste trabalho foi verificar se pressões usuais, em aplicações com o pulverizador de barra, podem afetar a ação inseticida de conídios de Beauveria bassiana e Metarhizium anisopliae, componentes de produtos comerciais brasileiros. Para isso, suspensões aquosas de três bioinseticidas foram submetidas à passagem pelo equipamento em três pressões (20, 40 e 60 lbf pol-2 e avaliadas quanto ao rompimento, à viabilidade e à virulência dos conídios. Avaliou-se a viabilidade em lâminas de microscopia cobertas com meio de cultura, após incubação a 26±0,5ºC e fotoperíodo de 12 horas. A concentração foi determinada por meio de contagens em câmara de Neubauer e a virulência foi avaliada para lagartas de Diatraea saccharalis. Não foram encontradas, nos três produtos, influências significativas em nenhum dos parâmetros. Nas pressões avaliadas, a aplicação com o pulverizador de barra não reduz a viabilidade e nem a virulência dos conídios dos bioinseticidas testados, tampouco provoca destruição dos conídios.Fungal biopesticides are applied with conventional sprayers and this equipment may be inappropriate, and therefore affect field control efficacy of biopesticides. The objective of this research was to find out if the usual pressures used in the boom sprayers can affect the conidia pesticide activity of the Brazilian commercial entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae. Water fungi suspensions were passed through the equipment under three pressures (20, 40 and 60 psi and evaluated for viability, virulence and conidia disruption. The viability was evaluated in slides covered by culture media after incubation at 26±0.5ºC and 12 hours photophase. The concentration was determined through Neubauer

  20. Nocturnal herbivore-induced plant volatiles attract the generalist predatory earwig Doru luteipes Scudder

    Science.gov (United States)

    Naranjo-Guevara, Natalia; Peñaflor, Maria Fernanda G. V.; Cabezas-Guerrero, Milton F.; Bento, José Maurício S.

    2017-10-01

    Numerous studies have demonstrated that entomophagous arthropods use herbivore-induced plant volatile (HIPV) blends to search for their prey or host. However, no study has yet focused on the response of nocturnal predators to volatile blends emitted by prey damaged plants. We investigated the olfactory behavioral responses of the night-active generalist predatory earwig Doru luteipes Scudder (Dermaptera: Forficulidae) to diurnal and nocturnal volatile blends emitted by maize plants ( Zea mays) attacked by either a stem borer ( Diatraea saccharalis) or a leaf-chewing caterpillar ( Spodoptera frugiperda), both suitable lepidopteran prey. Additionally, we examined whether the earwig preferred odors emitted from short- or long-term damaged maize. We first determined the earwig diel foraging rhythm and confirmed that D. luteipes is a nocturnal predator. Olfactometer assays showed that during the day, although the earwigs were walking actively, they did not discriminate the volatiles of undamaged maize plants from those of herbivore damaged maize plants. In contrast, at night, earwigs preferred volatiles emitted by maize plants attacked by D. saccharalis or S. frugiperda over undamaged plants and short- over long-term damaged maize. Our GC-MS analysis revealed that short-term damaged nocturnal plant volatile blends were comprised mainly of fatty acid derivatives (i.e., green leaf volatiles), while the long-term damaged plant volatile blend contained mostly terpenoids. We also observed distinct volatile blend composition emitted by maize damaged by the different caterpillars. Our results showed that D. luteipes innately uses nocturnal herbivore-induced plant volatiles to search for prey. Moreover, the attraction of the earwig to short-term damaged plants is likely mediated by fatty acid derivatives.

  1. The use of gamma radiation to control two serious pests of Brazilian agriculture

    International Nuclear Information System (INIS)

    Arthur, V.; Aguilar, J.A.D.; Wiendl, F.M.; Wiendl, T.A.

    2002-01-01

    This paper reports on the application of nuclear techniques to control two of the most important Lepidopteran insects pests in Brazil: Diatraea saccharalis, the sugarcane borer, and Spodoptera frugiperda, the fall armyworm. All experiments had the objective of finding the dose of gamma radiation capable of causing sterility in the first and second generations by irradiating the parental generation. For D. saccharalis, five day-old pupae were irradiated with doses of 100, 125 and 150 Gy. Fertility was reduced to 15% when moths were treated with 100 Gy and no egg hatch was recorded at 125 and 150 Gy. Fertility was 4.3% and 10.9% in the F 1 generation and was 9.5% and 25.5% in the F 2 generation, when treated males were mated to normal females and treated females were mated to normal males, respectively. The results of our research suggest a possible alternative tactic to control or even eradicate sugarcane borer from Brazil. For S. frugiperda, five day-old pupae were treated with doses of 50, 100, 125, 150 and 175Gy. Moths of the F 1 and F 2 generations were obtained only from parents treated at 50Gy. When higher doses were used, only the crosses where irradiated males were mated to normal (untreated) females produced moths of the F 1 and F 2 generations. Irradiation of the parental generation induced different sterility levels in the offspring. Female fall armyworm were more radiosensitive than males, and substerilizing doses of gamma radiation did not affect the life cycle of the first and second filial generations in this species. The level of sterility in the F 1 and F 2 generations was higher than the sterility of the parents irradiated at the same dose. These results are encouraging and indicate that inherited sterility might be used for control of this insect in Brazil. Large field experiments should be conducted to confirm the laboratory findings. (author)

  2. Manejo de lepidópteros-praga na cultura do milho com o evento Bt piramidado Cry1A.105 e Cry2Ab2

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    José Magid Waquil

    2013-12-01

    Full Text Available O objetivo deste trabalho foi avaliar a eficácia do evento piramidado (MON 89034, que expressa as proteínas Cry1A.105 e Cry2Ab2, no controle dos principais lepidópteros-praga da cultura do milho no Brasil, Spodoptera frugiperda, Helicoverpa spp. e Diatraea saccharalis. Os ensaios foram conduzidos em quatro regiões do país, com o híbrido DKB 390, submetido a seis tratamentos: híbrido com o evento piramidado, híbrido com o evento que expressa apenas a proteína Cry1A(b (MON 810 e híbrido convencional (não Bt, todos com e sem manejo integrado de S. frugiperda. Para o evento piramidado, não foi necessário o controle químico em nenhum dos locais avaliados. Diferenças significativas foram observadas entre os tratamentos quanto aos danos e à presença de lagartas. Em geral, essas variáveis foram mais baixas no híbrido com o evento piramidado e mais altas no híbrido convencional, sem controle químico. Sob alta infestação, o controle químico reduziu os danos causados por S. frugiperda e D. saccharalis, tanto no evento que expressa apenas uma proteína, como no híbrido convencional. Com base nos danos causados pelos insetos, o evento piramidado Cry1A.105 e Cry2Ab2 é eficiente no controle dos principais lepidópteros-pragas do milho no Brasil.

  3. Bacillus thuringiensis monogenic strains: screening and interactions with insecticides used against rice pests

    Science.gov (United States)

    Pinto, Laura M.N.; Dörr, Natália C.; Ribeiro, Ana Paula A.; de Salles, Silvia M.; de Oliveira, Jaime V.; Menezes, Valmir G.; Fiuza, Lidia M.

    2012-01-01

    The screening of Bacillus thuringiensis (Bt) Cry proteins with high potential to control insect pests has been the goal of numerous research groups. In this study, we evaluated six monogenic Bt strains (Bt dendrolimus HD-37, Bt kurstaki HD-1, Bt kurstaki HD-73, Bt thuringiensis 4412, Bt kurstaki NRD-12 and Bt entomocidus 60.5, which codify the cry1Aa, cry1Ab, cry1Ac, cry1Ba, cry1C, cry2A genes respectively) as potential insecticides for the most important insect pests of irrigated rice: Spodoptera frugiperda, Diatraea saccharalis, Oryzophagus oryzae, Oebalus poecilus and Tibraca limbativentris. We also analyzed their compatibility with chemical insecticides (thiamethoxam, labdacyhalothrin, malathion and fipronil), which are extensively used in rice crops. The bioassay results showed that Bt thuringiensis 4412 and Bt entomocidus 60.5 were the most toxic for the lepidopterans, with a 93% and 82% mortality rate for S. frugiperda and D. saccharalis, respectively. For O. oryzae, the Bt kurstaki NRD-12 (64%) and Bt dendrolimus HD-37 (62%) strains were the most toxic. The Bt dendrolimus HD-37 strain also caused high mortality (82%) to O. poecilus, however the strains assessed to T. limbativentris caused a maximum rate of 5%. The assays for the Bt strains interaction with insecticides revealed the compatibility of the six strains with the four insecticides tested. The results from this study showed the high potential of cry1Aa and cry1Ba genes for genetic engineering of rice plants or the strains to biopesticide formulations. PMID:24031872

  4. Sugarcane straw and the populations of pests and nematodes

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    Leila Luci Dinardo-Miranda

    2013-10-01

    Full Text Available The green cane harvesting represented a significant change in sugarcane ecosystem due to the presence of straw left on the soil and to the absence of fire. These two factors may affect the populations of pests and their natural enemies. Among the pests benefit from the green cane harvesting stand out the spittlebug, Mahanarva fimbriolata, the curculionid Sphenophorus levis and sugarcane borer, Diatraea saccharalis. In areas of green cane harvesting, the population of these species grew faster than in areas of burnt cane. On the other hand, there are virtually no records of attacks by lesser cornstalk borers in areas of green cane harvesting. Populations of plant parasitic nematodes and the beetles Migdolus fryanus, very important pests of sugarcane, were apparently not affected by the green cane harvesting. Despite the absence of more consistent information, it appears that populations of ants and the giant borer Telchin licus can increase in green cane areas, due primarily to the difficulty of pest control. The partial or total removal of straw from the field represents an additional change to the ecosystem that could alter the status of pests and nematodes. It is likely that spittlebug, the curculionid S. levis and sugarcane borer populations decrease if a portion of the straw is removed from the field. However, the pest populations in areas where the straw is collected will not return to their original conditions at the time of burnt cane harvesting because the absence of fire will be maintained.

  5. Possibly similar genetic basis of resistance to Bacillus thuringiensis Cry1Ab protein in 3 resistant colonies of the sugarcane borer collected from Louisiana, USA.

    Science.gov (United States)

    Yang, Fei; Chen, Mao; Gowda, Anilkumar; Kerns, David L; Huang, Fangneng

    2018-04-01

    The sugarcane borer, Diatraea saccharalis (F.), is a major maize borer pest and a target of transgenic maize expressing Bacillus thuringiensis (Bt) proteins in South America and the mid-southern region of the United States. Evolution of resistance in target pest populations is a great threat to the long-term efficacy of Bt crops. In this study, we compared the genetic basis of resistance to Cry1Ab protein in 3 resistant colonies of sugarcane borer established from field populations in Louisiana, USA. Responses of larvae to the Cry1Ab protein for the parental and 10 other cross colonies were assayed in a diet-incorporated bioassay. All 3 resistant colonies were highly resistant to the Cry1Ab protein with a resistance ratio of >555.6 fold. No maternal effect or sex linkage was evident for the resistance in the 3 colonies; and the resistance was functionally nonrecessive at the Cry1Ab concentrations of ≤ 3.16 μg/g, but it became recessive at ≥10 μg/g. In an interstrain complementation test for allelism, the F 1 progeny from crosses between any 2 of the 3 resistant colonies exhibited the similar resistance levels as their parental colonies, indicating that the 3 colonies most likely shared a locus of Cry1Ab resistance. Results generated from this study should provide useful information in developing effective strategies for managing Bt resistance in the insect. © 2016 Institute of Zoology, Chinese Academy of Sciences.

  6. Pest management program for sugarcane at the Sugar Enterprise “Melanio Hernández

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    Tamara Milagros Rodríguez Cardoso

    2014-07-01

    Full Text Available The research objective was to diagnose and identify pests, diseases and weeds in sugarcane cultivars at the Sugar Enterprise ‘Melanio Hernández’ in Sancti Spíritus in order to develop a pest management program that started from the identification of phytosanitary-related problems and the available resources in the agroecosystem. As primary pests, Diatraea saccharalis Fab., three species of rodents, the Yellowing Leaf Syndrome (YLS, Ustilago scitaminea H. Sydow and seven species of weeds, were identified. As secondary pests, the cuters species ( Leucania sp., L. unipuncta, L. inconspicua, L. cinericolis and the sugar cane rust(Puccinia melanocephala H.y P. Sydow., were identified. The levels of incidence of primary and secondary pests showed differences among the varieties and among the three agricultural cooperatives at the Enterprise. Seven species of natural enemy of borer (bioregulators Leucania spp and Mocis spp., primary or secondary crop pests, were observed to be associated to sugarcane. 16 plant species were determined to show repellent properties or phytopesticides in this sugar cane agroecosystem, but only Eucalyptus showed to have its push effect on borer. A system of pest management program for sugar cane was developed that included measures starting from the local resources identified in the agroecosystem.

  7. A technique for ecological studies of the cuban fly, 'Lixophaga diatraea' TOWNS., com o uso de tracador radioativo

    International Nuclear Information System (INIS)

    Sgrillo, R.B.; Wiendl, F.M; Walder, J.M.M; Arthur, V.

    1977-01-01

    The specific activity of 32 P required to label Cuban Fly (lixophaga diatraca) is determined. Results show that the radioisotope can be detected inside the bodies of G. mellonella host for at least 6 days, with effective half-life for the raioisotope of 2.8 days [pt

  8. Mensuração dos custos de laboratório para produção da vespa Cotesia flavipes - inimigo natural da broca da cana-de-açúcar na Usina Naviraí S.A.: uma análise comparativa = Laboratory costs for production of the wasp Cotesia flavipes - natural enemy of the sugar cane borer at the Navirai S.A. factory: A comparative analysis

    Directory of Open Access Journals (Sweden)

    Adélia Megumi Suguiyama

    2015-04-01

    Full Text Available O controle biológico usando parasitoides larvais e parasitóides de ovos específicos ou entomopatógenos (fungos, bactérias ou vírus, manipulados em criatórios artificiais e liberados massivamente para o controle da broca é o mais difundido. Dentre estes, no entanto, o que tem sido comumente utilizado em escala comercial é o controle biológico com parasitóides larvais. Neste contexto, a espécie Cotesia flavipes, é uma vespa parasitóide que combate a broca da cana-de-açúcar Diatraea saccharalis (Lepidóptera, uma praga que causa grandes perdas na produção da cana-de-açúcar. Em função da importância desse tipo de controle biológico, este trabalho tem como objetivo mensurar os custos do sistema de produção de Cotesia flavipes na Usina Naviraí S.A. – Açúcar e Álcool (USINAVI, localizada em Naviraí, Mato Grosso do Sul. Ressalta-se que devido aos danos causados pela broca, os prejuízos no campo chegam a 25% de perda da produtividade da cultura Neste sentido, como principais resultados do presente estudo, sugere-se: 1. Investir em treinamento e qualificação de pessoal de laboratório e de campo; 2. Pesquisas no sentido de ajustar à técnica de controle biológico às condições ecofisiológicas da região de plantio; 3. Controle rigoroso do processo em termos de assepsia e higienização. = The biological control using parasitoids and larval parasitoid eggs or specific entomopathogens (fungi, bacteria or viruses, manipulated in artificial breeding sites and released massively to control drill, is the most widespread. Among these, however, has commonly been used on a commercial scale is biological control using larval parasitoids. In this context, the Cotesia flavipes species is a parasitoid wasp that fighting drill cane sugar Diatraea saccharalis (Lepidoptera, a pest that causes major losses in the production of cane sugar. Because of the importance of this kind of biological control, this paper aims to measure the

  9. Development of a mathematical model for sugar cane borer population, Diatrea sacharalis (Fabr., 1794) and simulation of the Sterile Insect Technique (SIT)

    International Nuclear Information System (INIS)

    Sgrillo, R.B.

    1979-08-01

    The determination of the theoretical possibility of applying the Sterile Insect Technique (SIT) to control sugar cane borer, Diatraea saccharalis (Fabricius, 1794), population in the State of Sao Paulo was aimed at. This has been achieved with the development of a mathematical model of the insect population dynamics after simulation of the SIT. The model was constructed based on a field survey made in 1976 in four sugar cane regions of the State. With the surveys, data relative to insect population density of larvae and pupae was obtained. Data regarding fluctuation of adults and of some predators population were obtained using light traps. Through mathematical analysis of the data from the surveys it was noted that diapause occurred in large larvae. The percentage of larvae in diapause showed correlation with photoperiod and temperature. It was established that the number of degree days necessary for the insect to complement a generation is 954. A method was proposed to utilize the thermic constant concept equally for diapause conditions. A laboratory experiment showed that male adults irradiated at 50 krad gamma radiation ( 60 Co) produced a non-viable generation. Monthly mortality in each stage was estimated. From these data, sub-models were developed, correlating mortality with climatic and biological variables. The sub-models when grouped formed a model that permitted the simulation of the SIT. It was concluded that release of sterile insects in a number equal to those existing in the field, during the first three generations, would be an efficient method to control insect populations. Theoretically, a profit would be obtained if the cost for application of the method was up to Cr$ 1,355 per hectare. Release of sterile insects in a number nine times larger than those existing in the field during the first generation, would be equally efficient and a profit would be obtained if the cost for application of the method was Cr$ 975 per hectare. (Author) [pt

  10. Defense response in non-genomic model species: methyl jasmonate exposure reveals the passion fruit leaves' ability to assemble a cocktail of functionally diversified Kunitz-type trypsin inhibitors and recruit two of them against papain.

    Science.gov (United States)

    Botelho-Júnior, Sylvio; Machado, Olga L T; Fernandes, Kátia V S; Lemos, Francisco J A; Perdizio, Viviane A; Oliveira, Antônia E A; Monteiro, Leandro R; Filho, Mauri L; Jacinto, Tânia

    2014-08-01

    Multiplicity of protease inhibitors induced by predators may increase the understanding of a plant's intelligent behavior toward environmental challenges. Information about defense mechanisms of non-genomic model plant passion fruit (Passiflora edulis Sims) in response to predator attack is still limited. Here, via biochemical approaches, we showed its flexibility to build-up a broad repertoire of potent Kunitz-type trypsin inhibitors (KTIs) in response to methyl jasmonate. Seven inhibitors (20-25 kDa) were purified from exposed leaves by chromatographic techniques. Interestingly, the KTIs possessed truncated Kunitz motif in their N-terminus and some of them also presented non-consensus residues. Gelatin-Native-PAGE established multiple isoforms for each inhibitor. Significant differences regarding inhibitors' activity toward trypsin and chymotrypsin were observed, indicating functional polymorphism. Despite its rarity, two of them also inhibited papain, and such bifunctionality suggests a recruiting process onto another mechanistic class of target protease (cysteine-type). All inhibitors acted strongly on midgut proteases from sugarcane borer, Diatraea saccharalis (a lepidopteran insect) while in vivo assays supported their insecticide properties. Moreover, the bifunctional inhibitors displayed activity toward midgut proteases from cowpea weevil, Callosobruchus maculatus (a coleopteran insect). Unexpectedly, all inhibitors were highly effective against midgut proteases from Aedes aegypti a dipteran insect (vector of neglected tropical diseases) opening new avenues for plant-derived PIs for vector control-oriented research. Our results reflect the KTIs' complexities in passion fruit which could be wisely exploited by influencing plant defense conditions. Therefore, the potential of passion fruit as source of bioactive compounds with diversified biotechnological application was strengthened.

  11. Herbicidas e Reguladores de Crescimento de Plantas Utilizados na Cana-de-Açúcar e sua Ação sobre Adultos de Trichogramma galloi Zucchi (Hymenoptera: Trichogrammatidae

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    Marina Antigo

    2016-04-01

    Herbicides and Plant Growth Regulators Used in SUGARCANE and their Action on Adult Trichogramma galloi Zucchi (Hymenoptera: Trichogrammatidae Abstract. During crop sugarcane management, several used pesticides can affect the performance of parasitoids, natural enemies of sugarcane borer, Diatraea saccharallis (Fabricius and consequently the biological control ofit. Thus, the objective was to evaluate in laboratory the effect of herbicides and plant growth regulators used in the sugarcane crops on survival and parasitism in adults of the eggs parasitoids, Trichogramma galloi Zucchi. The products tested were the herbicides Clomazone and Diuron + Hexazinone, and the plant regulators Sulfometuron-methyl and Trinexapac-ethyl, all in the higher recommended dosages for sugarcane. The eggs of D. saccharalis were immersed during 5 seconds in chemical solutions and, after dry for 1 hour, offered to T. galloi females with 20 repetitions. We evaluated the mortality and number of eggs parasitized by the F0 generation of T. galloi, as well as the emergence of the F1. The products were classified by their toxicity, according to the percentage of reduction in the survival, parasitism and emergence of the parasitoid. In relation to the survival of adult T. galloi, the number of eggs parasitized by F0 as well as the percentage of F1 emergence, clomazone and trinexapac-ethyl were classified as slightly harmful. Diuron+hexazinone and sulfometuron-methyl were harmless in all parameters, except for parasitism, where diuron+hexazinone was classified as slightly harmful. We conclude that clomazone, diuron+hexazinone, trinexapac-ethyl and sulfometuron-methyl, are harmless or slightly harmful the survival and parasitism (F0 of T. galloi adults and emergence (F1 of this parasitoid in the laboratory.

  12. Assessment of the impact of the egg parasitoid, Paratelenoumus saccharalis (Hymenoptera: Platygastridae) on populations of the kudzu bug, Megacopta cribraria (Hemiptera: Plataspidae)

    Science.gov (United States)

    The kudzu bug has become a pest of economic importance ever since its introduction to the Southeastern United States from Asia in 2009. It causes serious economic damage to legume crops (soybeans, bean, pigeon pea, mung bean, velvet bean etc.) and is a nuisance to home owners. One natural egg parasi...

  13. PCR en tiempo real: una metodología útil para la detección y cuantificación de granulovirus

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    Gloria Patricia Barrera Cubillos

    2016-07-01

    Full Text Available El uso de baculovirus como agentes de control biológico de insectos plaga, se ha convertido en una estrategia efectiva que se ha implementado gradualmente en diferentes sistemas productivos a nivel mundial. Para el desarrollo de un bioplaguicida a base de baculovirus, es necesario contar con una metodología para determinar el título viral en el producto en proceso y terminado. Para tal fin, en este trabajo se diseñó y optimizó una técnica de cuantificación viral (Betabaculovirus mediante PCR cuantitativo (q-PCR. Se utilizó una sonda TaqMan diseñada sobre el gen de granulina, altamente conservado. Para la técnica de q-PCR se determinó la especificidad, sensibilidad y reproducibilidad, encontrando que puede detectar y cuantificar aislamientos del género Betabaculovirus provenientes de cinco especies diferentes de insectos (granulovirus de Tecia solanivora, Phthorimaea operculella, Erinnyis ello, Tuta absoluta y Spodoptera frugiperda incluso de diferente origen geográfico, pero no detecta aislamientos del género Alphabaculovirus (nucleopoliedrovirus de Spodoptera ornithogalli, Diatraea saccharalis o S. frugiperda. El límite mínimo de detección de la técnica fue de 6,4 x 10-4 ng de ADN, lo que equivale a 1,25 x 105 copias del gen. Así mismo, la variación intra e inter ensayos fue mínima, demostrando la reproducibilidad de la misma. La aplicabilidad de la técnica fue evaluada para la detección de granulovirus en muestras de larva, suelo, y para determinar la concentración viral en un bioplaguicida formulado como concentrado emulsionable. En conclusión, la técnica de q-PCR desarrollada fue reproducible, sensible y específica, con aplicabilidad en estudios de persistencia viral en campo, control de infecciones en crías de insectos y control de calidad de bioplaguicidas a base de betabaculovirus.

  14. Bioecologia de Euborellia annulipes (Dermaptera: Anisolabididae

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    Aldeni Barbosa da Silva

    2015-03-01

    Full Text Available 800x600 Dentre os agentes biológicos com boa capacidade predatória, os dermápteros têm despertado grande atenção, pois são predadores vorazes, isto é, com alta capacidade de ataque e que se alimentam de diversas presas, particularmente, de ovos e fases imaturas de insetos das ordens Lepidoptera, Hemiptera, Coleoptera e Diptera. São insetos terrestres, predadores, de hábitos noturnos, e às vezes vistos durante o dia. Os dermápteros têm se destacado no controle de insetos-praga, principalmente de ovos e formas jovens. Euborellia annulipes (Lucas é um agente controlador de Hyadaphis foeniculi (Passerini e Brevicoryne brassicae (Linnaeus, no Brasil; Cosmopolites sordidus (Germar, na Jamaica; lagartas de Sesamia inferens (Walker, no Japão, e alguns insetos-praga de grãos armazenados, da lagarta Crambus bonifatellus (Hulst, lagartas e pupas de Diatraea saccharalis (Fabricius, de ovos, larvas, ninfas e adultos de Dermanyssus gallinae (De Geer, Carpocapsa pomonella (Linné na França, de Icerya purchasi (Maskell na Califórnia e larvas e pupas de Anthonomus grandis (Boheman em nível de campo. Este trabalho teve o objetivo de fazer uma abordagem sobre a bioecologia de Euborellia annulipes, assim como, traçar considerações a respeito da sua classificação, de sua associação a pragas agrícolas e de seu uso em programas de controle biológico. Normal 0 21 false false false PT-BR X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabela normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman","serif";}

  15. Management of lepidopterans through irradiations

    International Nuclear Information System (INIS)

    Bhati, Dheeraj; Parvez, Asif; Kausar, Hina; Srivastava, Meera

    2012-01-01

    suppression by irradiation include Spodoptera litura, S.exigua, S.frugiperda, Helicoverpa armigera, H.zea, Pectinophora gossypiella, Diatraea saccharalis, Cydia pomonella, C.molesta, Ectomyelois ceratoniae, Ephestia kuehniella, Crocidolomia binotalis, Chilo suppressalis, Ostrinia furnacalis, O.nubilalis, Plutella xylostella and Spilosoma obliqua. (author)

  16. Lack of Detection of Bt Sugarcane Cry1Ab and NptII DNA and Proteins in Sugarcane Processing Products Including Raw Sugar

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    Adriana Cheavegatti-Gianotto

    2018-03-01

    Full Text Available Brazil is the largest sugarcane producer and the main sugar exporter in the world. The industrial processes applied by Brazilian mills are very efficient in producing highly purified sugar and ethanol. Literature presents evidence of lack of DNA/protein in these products, regardless of the nature of sugarcane used as raw material. Recently CTNBio, the Brazilian biosafety authority, has approved the first biotechnology-derived sugarcane variety for cultivation, event CTC175-A, which expresses the Cry1Ab protein to control the sugarcane borer (Diatraea saccharalis. The event also expresses neomycin-phosphotransferase type II (NptII protein used as selectable marker during the transformation process. Because of the high purity of sugar and ethanol produced from genetically modified sugarcane, these end-products should potentially be classified as “pure substances, chemically defined,” by Brazilian Biosafety Law No. 11.105. If this classification is to be adopted, these substances are not considered as “GMO derivatives” and fall out of the scope of Law No. 11.105. In order to assess sugar composition and quality, we evaluate Cry1Ab and NptII expression in several sugarcane tissues and in several fractions from laboratory-scale processing of event CTC175-A for the presence of these heterologous proteins as well as for the presence of traces of recombinant DNA. The results of these studies show that CTC175-A presents high expression of Cry1Ab in leaves and barely detectable expression of heterologous proteins in stalks. We also evaluated the presence of ribulose-1,5-bisphosphate carboxylase/oxygenase protein and DNA in the fractions of the industrial processing of conventional Brazilian sugarcane cultivars. Results from both laboratory and industrial processing were concordant, demonstrating that DNA and protein are not detected in the clarified juice and downstream processed fractions, including ethanol and raw sugar, indicating that protein

  17. Melhoramento da cana-de-açúcar: IX: evaluation of clones obtained in 1980 and 1981 hybridizations, selected in Ribeirão Preto region, state of São Paulo, Brazil Sugarcane breeding: IX

    Directory of Open Access Journals (Sweden)

    Marcos Guimarães de Andrade Landell

    1995-01-01

    Full Text Available Testaram-se doze clones de cana-de-açúcar, obtidos de hibridações realizadas em Camamu (BA, em 1980 e 1981, em três ensaios na região de Ribeirão Preto (SP. Além do delineamento em blocos ao acaso, com seis repetições, efetuou-se a análise estatística com a média das três colheitas (1.°, 2.° e 3.° cortes, tomando as variedades SP70-1143, NA56-79 e IAC64-257 como testemunhas-padrão. Entre os caracteres agroindustriais avaliados estão: a produtividade de cana e açúcar, pol % cana, fibra %, intensidade de florescimento, índice de infestação de broca-do-colmo (Diatraea saccharalis e a reação à ferrugem (Puccinia melanocephala. O melhor clone foi o IAC80-2094, indicado para início de safra, com boa produção e bom teor de fibra, mas de florescimento intenso e suscetibilidade à ferrugem. O IAC81-2004 também apresentou bons resultados, caracterizando-se como precoce, com bom teor de fibra e boa resistência à broca-do-colmo. Em condições naturais de campo, porém, sua desvantagem é a grande incidência de "chicotes" de carvão. Apesar de ambos os clones apresentarem características agroindustriais vantajosas, desaconselha-se que sejam incluídos no estudo de manejo parietal para outras regiões paulistas, em função dos problemas fitossanitários citados.A number of sugarcane clones obtained in crosses made in 1980 and 1981, was tested in three locations with Oxisol soils at Ribeirão Preto region. The commercial varieties SP70-1143, NA56-79 and IAC64-257 were used as controls in trials and evaluated for agricultural and industrial traits on the average of three harvests. The best clone in the experiments was IAC80-2094, which has been indicated for early harvest period with good yield and fiber content, but with heavy tasseling and susceptibility to rust. Other early maturing clone was IAC81-2004, which showes good fiber content and stem borer tolerance, however it does show, in natural conditions in the field

  18. Seasonal Occurrence and Interspecific Interactions of Egg Parasitoids of Megacopta cribraria (Heteroptera: Plataspidae) in Japan.

    Science.gov (United States)

    Hoshino, Keisuke; Adati, Tarô; Olson, Dawn M; Takasu, Keiji

    2017-06-01

    We conducted a field study to determine seasonal egg parasitism rates of the kudzu bug Megacopta cribraria (F.) on the kudzu plant, Pueraria montana (Lour.) Merr. var. lobata (Willd.) Maesen et Almeida ex Sanjappa and Pradeep, in Tokyo, Japan, during the period from May 2014 to September 2014. The eggs of M. cribraria per 1 m2 of kudzu at four locations in Tokyo were collected weekly and parasitism rates were assessed. Eggs of M. cribraria were laid on the kudzu plant from May to September. Megacopta cribraria eggs were parasitized by two parasitoid species, Paratelenomus saccharalis (Dodd) and Ooencyrtus nezarae Ishii. Paratelenomus saccharalis first appeared in May, and its parasitism rates peaked in July and September. Ooencyrtus nezarae first appeared in June and its parasitism rates peaked in July. Except for one location which could not be statistically analyzed because of the small sample size, occurrence of parasitism by P. saccharalis and O. nezarae in M. cribraria egg masses was independent at one location and positively associated at two locations, suggesting that the use of host egg masses by P. saccharalis and O. nezarae is not mutually exclusive. Parasitism rates by P. saccharalis and O. nezarae were significantly lower for egg masses parasitized by both species than for those parasitized by a single species. The proportion of males among O. nezarae progeny was significantly higher for egg masses parasitized by O. nezarae together with P. saccharalis than for those parasitized by O. nezarae alone. These results suggest that parasitism of host egg masses by the two species is influenced by their interspecific interactions. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  19. Effect of acaricidal activity of Solanum nigrum on Tetranychus ...

    African Journals Online (AJOL)

    sunny t

    2016-03-09

    Mar 9, 2016 ... biological control and plant extracts (Oliveira et al.,. 2007). The use of plant extracts may be an alternative and environmentally safe strategy of pest ..... Alves SB, Rossi LS, Biaggoni LR, Tamai MA, Pereira PM (2002). Beauveria bassiana yeast phase on agar medium and its pathogenicity against Diatraea ...

  20. Mapping of quantitative trait loci for resistance to fall armyworm and southwestern corn borer leaf-feeding damage in maize.

    Science.gov (United States)

    Fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith), and southwestern corn borer (SWCB), Diatraea grandiosella Dyar are damaging insect pests of maize resulting in significant yield and economic losses. A previous study identified quantitative trait loci (QTL) that contribute to reduced leaf-fe...

  1. Effects of the gamma and ultraviolet radiation in metarhizium anisopliae (METSCH) isolated SOROKIN, 1883 and its application to control the distracer saccharals (Fabricius, 1974)

    International Nuclear Information System (INIS)

    Almeida, L.C. de.

    1983-06-01

    The objective of this work was to study the effects of gamma and ultraviolet radiations in isolates of M. anisopliae and the utilization of this pathogen, aiming the polulation control of the sugarcane borer, D. saccharalis. To evaluate the application of M. anisopliae under field conditions, two experiments were carried out, the first consisting of a spore suspension application upon sugarcane borer egg masses, and the second consisting of a pulverization of spores upon egg masses previously placed on sugarcane leaves. The dosages utilized were of 100, 200 and 300 g of spores/ha. The results obtained in both trials indicated that the deposition of spores on D. saccharalis egg masses was difficulted by the sugarcane foliar mass. (author) [pt

  2. Interaction between N-fertilizer and water availability on borer-rot complex in sugarcane

    Directory of Open Access Journals (Sweden)

    Luiz Eduardo da Rocha Pannuti

    2015-03-01

    Full Text Available This study aimed to evaluate the effects of nitrogen availability in fertigation and rainfed management, as well as their interactions with the incidence of and damage caused by D. saccharalis and red rot in sugarcane. The experiment consisted of four treatments (0 and 150 kg ha–1 of N-fertilizer with irrigation; 0 and 150 kg ha–1 of N-fertilizer in rainfed management in a randomized complete block design with four replications. The evaluated parameters were the number of holes and internodes with red rot per meter of cultivation, stalk yield and sugar content. In the laboratory (T = 25 ± 2 °C; R.H. = 70 ± 10%: 12:12-L:D, we evaluated the attractiveness and consumption of fragments of stalks from the different treatments for fourth instar larvae through choice and no-choice tests in a randomized complete block design with ten replications. Nitrogen fertilization via irrigation has favorable effects on borer-rot complex and leads to higher gains in stalk and sugar yields when compared to rainfed management. The increments of stalk and sugar yields due to nitrogen fertilization compensates for the increase in borer-rot complex infestation. In laboratory tests, D. saccharalis larvae were similarly attracted to all treatments regardless of the doses of N-fertilizer or the water regimes evaluated. However, fragments of sugarcane stalks produced with nitrogen fertilization were consumed more by D. saccharalis in both water regimes.

  3. Enzymatic, Antioxidant, Antimicrobial, and Insecticidal Activities of Pleurotus pulmonarius and Pycnoporus cinnabarinus Grown Separately in an Airlift Reactor

    Directory of Open Access Journals (Sweden)

    Maura Téllez-Téllez

    2016-03-01

    Full Text Available Crude extract samples of Pleurotus pulmonarius and Pycnoporus cinnabarinus were taken during growth in liquid broth in an airlift reactor. Growth was monitored indirectly by sugar consumption and pH profile. During growth Pleurotus pulmonarius consumed glucose more slowly than Pycnoporus cinnabarinus, reaching a final pH of 8.0. In contrast, Pycnoporus cinnabarinus started consuming glucose faster from the beginning to the end with a pH of 3.6, suggesting the production of different metabolites while they grow in the same culture broth. Additionally, antioxidant activity, polyphenol and flavonoid contents, as well as laccase and hydrolase activities were quantified in the culture extracts during the fermentation. Pleurotus pulmonarius showed higher antioxidant activity than Pycnoporus cinnabarinus. Both fungi have a very low polyphenol and flavonoid content. Values of amylase and pectinase activities were similar in crude extracts of both fungi; however, cellulase, xylanase, invertase, and laccase activities showed higher levels in crude extract of Pleurotus pulmonarius. Antimicrobial and insecticidal activities were also evaluated in each crude extract. In fact, Pycnoporus cinnabarinus presented a very strong bacteriostatic and bactericidal effect against Escherichia coli and Staphylococcus aureus and reliably killed Diatraea magnifactella larvae, while Pleurotus pulmonarius did not showed any negative effect on the growth of these bacteria or larvae.

  4. Secretome Analysis of Metarhizium anisopliae Under Submerged Conditions Using Bombyx mori Chrysalis to Induce Expression of Virulence-Related Proteins.

    Science.gov (United States)

    Rustiguel, Cynthia Barbosa; Rosa, José Cesar; Jorge, João Atílio; de Oliveira, Arthur Henrique Cavalcanti; Guimarães, Luis Henrique Souza

    2016-02-01

    The entomopathogenic fungus Metarhizium anisopliae is used to control insect pests. This species is specialized for the secretion of an enzymatic complex consisting of proteases, lipases, and chitinases related to pathogenicity and virulence. In this context, the secretomes of strains IBCB 167 and IBCB 384 of M. anisopliae var. anisopliae, grown under submerged fermentation in the presence of chrysalis as an inducer, were analyzed. Analysis of two-dimensional gels showed qualitative and quantitative differences between secreted proteins in both isolates. Around 102 protein spots were analyzed, and 76 % of the corresponding proteins identified by mass spectrometry were grouped into different classes (hydrolases, oxidases, reductases, isomerases, kinases, WSC domains, and hypothetical proteins). Thirty-three per cent of all the proteins analyzed were found to be common in both strains. Several virulence-related proteins were identified as proteases and mannosidases. Endo-N-acetyl-β-D-glucosaminidase expression was observed to be 10.14-fold higher for strain IBCB 384 than for strain IBCB 167, which may be an important contributor to the high virulence of IBCB 384 in Diatraea ssaccharalis. These results are important for elucidation of the host-pathogen relationship and the differences in virulence observed between the two strains.