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Sample records for campion silene latifolia

  1. Identification of white campion (Silene latifolia guaiacol O-methyltransferase involved in the biosynthesis of veratrole, a key volatile for pollinator attraction

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    Gupta Alok K

    2012-08-01

    Full Text Available Abstract Background Silene latifolia and its pollinator, the noctuid moth Hadena bicruris, represent an open nursery pollination system wherein floral volatiles, especially veratrole (1, 2-dimethoxybenzene, lilac aldehydes, and phenylacetaldehyde are of key importance for floral signaling. Despite the important role of floral scent in ensuring reproductive success in S. latifolia, the molecular basis of scent biosynthesis in this species has not yet been investigated. Results We isolated two full-length cDNAs from S. latifolia that show similarity to rose orcinol O-methyltransferase. Biochemical analysis showed that both S. latifolia guaiacol O-methyltransferase1 (SlGOMT1 &S. latifolia guaiacol O-methyltransferase2 (SlGOMT2 encode proteins that catalyze the methylation of guaiacol to form veratrole. A large Km value difference between SlGOMT1 (~10 μM and SlGOMT2 (~501 μM resulted that SlGOMT1 is 31-fold more catalytically efficient than SlGOMT2. qRT-PCR expression analysis showed that the SlGOMT genes are specifically expressed in flowers and male S. latifolia flowers had 3- to 4-folds higher level of GOMT gene transcripts than female flower tissues. Two related cDNAs, S. dioica O-methyltransferase1 (SdOMT1 and S. dioica O-methyltransferase2 (SdOMT2, were also obtained from the sister species Silene dioica, but the proteins they encode did not methylate guaiacol, consistent with the lack of veratrole emission in the flowers of this species. Our evolutionary analysis uncovered that SlGOMT1 and SlGOMT2 genes evolved under positive selection, whereas SdOMT1 and SdOMT2 genes show no evidence for selection. Conclusions Altogether, we report the identification and functional characterization of the gene, SlGOMT1 that efficiently catalyzes veratrole formation, whereas another copy of this gene with only one amino acid difference, SlGOMT2 was found to be less efficient for veratrole synthesis in S. latifolia.

  2. Effects of pollination timing and distance on seed production in a dioecious weed Silene latifolia

    Science.gov (United States)

    Anderson, Jay F.; Duddu, Hema S. N.; Shirtliffe, Steven J.; Benaragama, Dilshan; Syrovy, Lena D.; Stanley, Katherine A.; Haile, Teketel A.

    2015-11-01

    Silene latifolia Poir. (white cockle or white campion) is an important invasive weed in North American agriculture. It exhibits dioecy, therefore, both male and female plants are required in order for seed production to occur. However, dioecious species being invasive is not common because of their limitations in pollination and subsequent seed production. The objective of this study is to determine the effect of pollination timing and distance on seed production of Silene latifolia. A series of experiments including pollination exclusion, timing and pollination distance were conducted in 2009 and 2010 at or around Saskatoon, Saskatchewan. For pollination exclusion, exclosures were built around the natural female plants for exclosure, sham-exclosure, and male and female combined treatments. Pollination timing was studied by applying exclosure, non-exclosure, night-exclosure, and day-exclosure treatments to individual female plants. Female plants were transplanted along a linear interval at six different distances from the pollen source to study the effect of pollination distance. S. latifolia was exclusively insect-pollinated and pollination occurred both day and night; however, in one year, pollination occurred mainly at night. Female plants that were in the range of 0-4 m from a compatible pollen source experienced no limitation to pollination. However, when the distance was increased further up to 128 m, pollination levels and subsequent seed production were declined. Moreover, there were differences in seed production between years suggesting that pollination was affected by the environmental conditions during pollination and the crop that white cockle was grown in. These experiments indicate that seed production in S. latifolia is limited by insect-pollination. Although there was pollination limitation for seed production at greater distances from a pollen source, the high fecundity rate (3000-18000 seeds per plant) resulted in a large seed output. Thus, we

  3. On flavonoid accumulation in different plant parts: Variation patterns among individuals and populations in the shore campion (Silene littorea.

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    Jose Carlos Del Valle

    2015-10-01

    Full Text Available The presence of anthocyanins in flowers and fruits is frequently attributed to attracting pollinators and dispersers. In vegetative organs, anthocyanins and other non-pigmented flavonoids such as flavones and flavonols may serve protective functions against UV radiation, cold, heat, drought, salinity, pathogens and herbivores; thus, these compounds are usually produced as a plastic response to such stressors. Although the independent accumulation of anthocyanins in reproductive and vegetative tissues is commonly postulated due to differential regulation, the accumulation of flavonoids within and among populations has never been thoroughly compared. Here, we investigated the shore campion (Silene littorea, Caryophyllaceae which exhibits variation in anthocyanin accumulation in its floral and vegetative tissues. We examined the in-situ accumulation of flavonoids in floral (petals and calyxes and vegetative organs (leaves from 18 populations representing the species’ geographic distribution. Each organ exhibited considerable variability in the content of anthocyanins and other flavonoids both within and among populations. In all organs, anthocyanin and other flavonoids were correlated. At the plant level, the flavonoid content in petals, calyxes and leaves was not correlated in most of the populations. However, at the population level, the mean amount of anthocyanins in all organs was positively correlated, which suggests that the variable environmental conditions of populations may play a role in anthocyanin accumulation. These results are unexpected because the anthocyanins are usually constitutive in petals, yet contingent to environmental conditions in calyxes and leaves. Anthocyanin variation in petals may influence pollinator attraction and subsequent plant reproduction, yet the amount of anthocyanins may be a direct response to environmental factors. In populations on the west coast, a general pattern of increasing accumulation of

  4. Comparative analysis of a plant pseudoautosomal region (PAR in Silene latifolia with the corresponding S. vulgaris autosome

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    Blavet Nicolas

    2012-06-01

    Full Text Available Abstract Background The sex chromosomes of Silene latifolia are heteromorphic as in mammals, with females being homogametic (XX and males heterogametic (XY. While recombination occurs along the entire X chromosome in females, recombination between the X and Y chromosomes in males is restricted to the pseudoautosomal region (PAR. In the few mammals so far studied, PARs are often characterized by elevated recombination and mutation rates and high GC content compared with the rest of the genome. However, PARs have not been studied in plants until now. In this paper we report the construction of a BAC library for S. latifolia and the first analysis of a > 100 kb fragment of a S. latifolia PAR that we compare to the homologous autosomal region in the closely related gynodioecious species S. vulgaris. Results Six new sex-linked genes were identified in the S. latifolia PAR, together with numerous transposable elements. The same genes were found on the S. vulgaris autosomal segment, with no enlargement of the predicted coding sequences in S. latifolia. Intergenic regions were on average 1.6 times longer in S. latifolia than in S. vulgaris, mainly as a consequence of the insertion of transposable elements. The GC content did not differ significantly between the PAR region in S. latifolia and the corresponding autosomal region in S. vulgaris. Conclusions Our results demonstrate the usefulness of the BAC library developed here for the analysis of plant sex chromosomes and indicate that the PAR in the evolutionarily young S. latifolia sex chromosomes has diverged from the corresponding autosomal region in the gynodioecious S. vulgaris mainly with respect to the insertion of transposable elements. Gene order between the PAR and autosomal region investigated is conserved, and the PAR does not have the high GC content observed in evolutionarily much older mammalian sex chromosomes.

  5. FAST-FISH with laser beam microdissected DOP-PCR probe distinguishes the sex chromosomes of Silene latifolia.

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    Hobza, Roman; Lengerova, Martina; Cernohorska, Halina; Rubes, Jiri; Vyskot, Boris

    2004-01-01

    We present an improved FISH strategy for differentiating the sex chromosomes of the dioecious model plant, Silene latifolia. Fixed mitotic protoplasts were dropped on a polyethylene naphthalate membrane, the X or Y chromosomes were isolated using nitrogen laser beam microdissection, catapulted by laser pressure, and amplified by DOP-PCR. A modified FAST-FISH protocol based on a short hybridization time combined with a low concentration of probe was used. The success of this approach is demonstrated by the differential labeling of the X and Y chromosomes and it could represent a quick method for comparing organization of plant genomes. PMID:15125638

  6. Selection on floral characters in natural Spanish populations of Silene latifolia.

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    Wright, J W; Meagher, T R

    2004-03-01

    In insect-pollinated plants, floral characters are expected to play an important role in paternal and maternal reproductive success. Bateman's principle states that male reproductive success increases with more mating opportunities, while female reproductive success is limited by the amount of resources available to produce progeny, thus there should be greater selection on male floral characters than on female. In the case of the dioecious plant Silene latifolia, floral characters are likely to be influenced by its association within its native European range with moths of the genus Hadena, which serve as both pollinators and seed predators. The present study addresses relationships between male and female reproductive success, spatial location and floral characters (corolla, calyx and claw) over a 2-year period in two Spanish populations of S. latifolia in the presence of Hadena moths. A maximum likelihood paternity analysis using genetic variation at six allozyme markers showed heterogeneity in male reproductive success. There was much less variation in female reproductive success. When this analysis was extended to include interplant distance as a causal factor underlying variation in male success, we found that successful pollination tended to be limited to nearby females, in accordance with exponential decay of pollen dispersal with increasing distance. When the paternity analysis included floral characters as a causal factor underlying variation in male success, our data showed little evidence for directional selection, but there was stabilizing selection in one of the two years for calyx diameter. Selection on female characters varied widely between sites and years, in most of the site/year combinations there was little selection on female floral characters; however, in one site/year there was evidence for selection on all three floral characters. We conclude that pollinators visit flowers that are close together, and that while floral characters are

  7. A new physical mapping approach refines the sex-determining gene positions on the Silene latifolia Y-chromosome

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    Kazama, Yusuke; Ishii, Kotaro; Aonuma, Wataru; Ikeda, Tokihiro; Kawamoto, Hiroki; Koizumi, Ayako; Filatov, Dmitry A.; Chibalina, Margarita; Bergero, Roberta; Charlesworth, Deborah; Abe, Tomoko; Kawano, Shigeyuki

    2016-01-01

    Sex chromosomes are particularly interesting regions of the genome for both molecular genetics and evolutionary studies; yet, for most species, we lack basic information, such as the gene order along the chromosome. Because they lack recombination, Y-linked genes cannot be mapped genetically, leaving physical mapping as the only option for establishing the extent of synteny and homology with the X chromosome. Here, we developed a novel and general method for deletion mapping of non-recombining regions by solving “the travelling salesman problem”, and evaluate its accuracy using simulated datasets. Unlike the existing radiation hybrid approach, this method allows us to combine deletion mutants from different experiments and sources. We applied our method to a set of newly generated deletion mutants in the dioecious plant Silene latifolia and refined the locations of the sex-determining loci on its Y chromosome map.

  8. The large-X effect in plants: increased species divergence and reduced gene flow on the Silene X-chromosome.

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    Hu, Xin-Sheng; Filatov, Dmitry A

    2016-06-01

    The disproportionately large involvement of the X-chromosome in the isolation of closely related species (the large-X effect) has been reported for many animals, where X-linked genes are mostly hemizygous in the heterogametic sex. The expression of deleterious recessive mutations is thought to drive the frequent involvement of the X-chromosome in hybrid sterility, as well as to reduce interspecific gene flow for X-linked genes. Here, we evaluate the role of the X-chromosome in the speciation of two closely related plant species - the white and red campions (Silene latifolia and S. dioica) - that hybridize widely across Europe. The two species evolved separate sexes and sex chromosomes relatively recently (~10(7)  years), and unlike most animal species, most X-linked genes have intact Y-linked homologs. We demonstrate that the X-linked genes show a very small and insignificant amount of interspecific gene flow, while gene flow involving autosomal loci is significant and sufficient to homogenize the gene pools of the two species. These findings are consistent with the hypothesis of the large-X effect in Silene and comprise the first report of this effect in plants. Nonhemizygosity of many X-linked genes in Silene males indicates that exposure of recessive mutations to selection may not be essential for the occurrence of the large-X effect. Several possible causes of the large-X effect in Silene are discussed.

  9. Genetic diversity in Silene sennenii Pau (Caryophyllaceae assayed through DNA-based techniques

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    Blanché, C.

    2012-12-01

    Full Text Available Silene sennenii is a narrow endemic species from the NE of the Iberian Peninsula. It is considered as EN (“Endangered” according to the IUCN criteria and is under legal protection in Catalonia. In the present work we report an assay using three different approaches for surveying the genetic diversity in this rare, endangered campion: analysis of chloroplast haplotypes, AFLPs and transferability of microsatellites previously developed for Silene latifolia. None of the nine chloroplast regions sequenced showed any variability. Five out of the 21 microsatellite loci tested were polymorphic although more loci are required in order to perform a robust population genetics study. Regarding the AFLP analysis, five out of the 26 pairs of primers tested exhibited moderate levels of variability and therefore they could be useful for further investigating the genetic structure of S. sennenii. Although preliminary, our results based on three different genetic markers are in agreement with the low values of genetic variation already reported for this species employing allozymes.Silene sennenii es una especie endémica, circunscrita a un área extremadamente reducida al NE de la Península Ibérica. Está catalogada como EN («En Peligro» según criterios UICN y se encuentra legalmente protegida en Cataluña. En el presente trabajo se expone el ensayo de tres aproximaciones diferentes al estudio de su diversidad genética: análisis de haplotipos cloroplásticos, AFLPs y transferibilidad de microsatélites diseñados previamente para Silene latifolia. Ninguna de las nueve regiones cloroplásticas secuenciadas ha presentado variabilidad. Se han obtenido cinco loci microsatélites polimórficos de los 21 ensayados, cantidad insuficiente para llevar a cabo un estudio robusto sobre genética poblacional. En cuanto a AFLPs, cinco combinaciones de cebadores de las 26 probadas han mostrado niveles moderados de variabilidad siendo así útiles para posteriores

  10. Sexual dimorphism in white campion: complex control of carpel number is revealed by Y chromosome deletions

    International Nuclear Information System (INIS)

    Sexual dimorphism in the dioecious plant white campion (Silene latifolia = Melandrium album) is under the control of two main regions on the Y chromosome. One such region, encoding the gynoecium-suppressing function (GSF), is responsible for the arrest of carpel initiation in male flowers. To generate chromosomal deletions, we used pollen irradiation in male plants to produce hermaphroditic mutants (bsx mutants) in which carpel development was restored. The mutants resulted from alterations in at least two GSF chromosomal regions, one autosomal and one located on the distal half of the (p)-arm of the Y chromosome. The two mutations affected carpel development independently, each mutation showing incomplete penetrance and variegation, albeit at significantly different levels. During successive meiotic generations, a progressive increase in penetrance and a reduction in variegation levels were observed and quantified at the level of the Y-linked GSF (GSF-Y). Possible mechanisms are proposed to explain the behavior of the bsx mutations: epigenetic regulation or/and second-site mutation of modifier genes. In addition, studies on the inheritance of the hermaphroditic trait showed that, unlike wild-type Y chromosomes, deleted Y chromosomes can be transmitted through both the male and the female lines. Altogether, these findings bring experimental support, on the one hand, to the existence on the Y chromosome of genic meiotic drive function(s) and, on the other hand, to models that consider that dioecy evolved through multiple mutation events. As such, the GSF is actually a system containing more than one locus and whose primary component is located on the Y chromosome

  11. Analisi di un campione significativo di Learning Objects.

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    Luigi Guerra

    2006-01-01

    Full Text Available Il lavoro analizza sul piano pedagogico e didattico il concetto di Learning Object (LO a partire dalla rilevazione diretta dei modelli formativi sottostanti, in forma esplicita o implicita, un campione significativo di Learning Objects identificato all’interno di rilevanti esperienze di e-learning universitarie e non universitarie prodotte in diverse nazioni, allo scopo di giungere alla messa a punto di una modellistica problematica di tipo didattico dei Learning Objects, funzionale alla costruzione di algoritmi differenziati di progettazione, realizzazione, erogazione e valutazione di LO, anche nella prospettiva dei Learning Artifacts.

  12. Molecular and Quantitative Genetic Differentiation in European Populations of Silene latifolia (Caryophyllaceae)

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    Jolivet, Céline; Bernasconi, Giorgina

    2009-01-01

    Background and Aims: Among-population differentiation in phenotypic traits and allelic variation is expected as a consequence of isolation, drift, founder effects and local selection. Therefore, investigating molecular and quantitative genetic divergence is a pre-requisite for studies of local adaptation in response to selection under variable environmental conditions. Methods: Among- and within-population variation were investigated in six geographically separated European populations of t...

  13. History of the invasion of the anther smut pathogen on Silene latifolia in North America

    NARCIS (Netherlands)

    Fontaine, Michael C; Gladieux, Pierre; Hood, Michael E; Giraud, Tatiana

    2013-01-01

    Understanding the routes of pathogen introduction contributes greatly to efforts to protect against future disease emergence. Here, we investigated the history of the invasion in North America by the fungal pathogen Microbotryum lychnidis-dioicae, which causes the anther smut disease on the white ca

  14. Glycosides from the Leaves of Ilex latifolia

    Institute of Scientific and Technical Information of China (English)

    OUYANG,Ming-An(欧阳明安); OUYANG,Ming-An

    2001-01-01

    Nine new triterpenoid saponins: lafifolosides I-Q and three known conpouds: Kudinoside A, cis-roseoside, and kaenmpferol-3-O-a-L-rhamnopyranosyl ( 1→6 )-O-β-D-glucopyanoside were isolated from the leaves of ;lex latifolia.Their structures were elucidated by spectroscopic and chemical methods.

  15. SUSPENSION CULTURE AND PLANT REGENERATION OF TYPHA LATIFOLIA

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    This study is the first reported attempt to generate a growth curve from Typha latifolia L. (broadleaf cattail) callus cells in suspension culture. Several media and hormone combinations were tested for their capacity to induce callus cell formation from T. latifolia leaf section...

  16. [Examples of the keeping of drugs during the XVIth, XVIIth and XVIIIth centuries: silenes and drawers].

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    Fievet, B

    1998-01-01

    Silenes are wooden boxes with colourful paintings for the keeping of drugs and can be found nowadays in three collections in France: Hotel-Dieu in Troyes (Aube), a pharmacy in Bauge (Maine-et-Loire) and St-Roch museum in Issoudun (Indre). Drawers can be related to silenes but they are more ordinary and less attractive.

  17. Pharmacognostic standardisation of Hilleria latifolia (Lam.) H. Walt. (Phytolaccaceae)

    Institute of Scientific and Technical Information of China (English)

    Isaac Kingsley Amponsah; AbrahamYeboah Mensah; Antonia Otoo; Merlin Lincoln Kwao Mensah; Jato Jonathan

    2014-01-01

    To establish the pharmacognostic characters for the correct identification and quality control of Hilleria latifolia (H. latifolia), an important herb in Ghanaian folklore medicine, for the treatment of infections, pain and inflammation. Methods: The macro-morphological, qualitative and quantitative microscopic features, physicochemical and phytochemical features of the medicinally used parts of H. latifolia were evaluated using standard methods. Results: The plant has simple, alternate leaves with entire margin. The lamina is ovate to broadly lanceolate with an acuminate apex. It is hypostomatic with anomocytic stomata. The plant contains abundant prismatic crystals in all parts. Starch grains abound in the roots. The quantitative indices of the leaf and physicochemical parameters have also been established. Conclusions: The pharmacognostic features established in this study may be used as part of the pharmacopoeial standard for the correct identification and quality control of H. latifolia.

  18. A new isoflavone from the fruits of Madhuca latifolia.

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    Siddiqui, Bina S; Khan, Shazia; Nadeem Kardar, M

    2010-01-01

    A new isoflavone, 3',4'-dihydroxy-5,2'-dimethoxy-6,7-methylendioxy isoflavone, was isolated from the Madhuca latifolia fruit coatings. The structure was elucidated on the basis of spectral and chemical evidence. PMID:20013476

  19. "Initial investigation into computer scoring of candidate essays for personnel selection": Correction to Campion et al. (2016).

    Science.gov (United States)

    2016-07-01

    Reports an error in "Initial Investigation Into Computer Scoring of Candidate Essays for Personnel Selection" by Michael C. Campion, Michael A. Campion, Emily D. Campion and Matthew H. Reider (Journal of Applied Psychology, Advanced Online Publication, Apr 14, 2016, np). In the article the affiliations for Emily D. Campion and Matthew H. Reider were originally incorrect. All versions of this article have been corrected. (The following abstract of the original article appeared in record 2016-18130-001.) Emerging advancements including the exponentially growing availability of computer-collected data and increasingly sophisticated statistical software have led to a "Big Data Movement" wherein organizations have begun attempting to use large-scale data analysis to improve their effectiveness. Yet, little is known regarding how organizations can leverage these advancements to develop more effective personnel selection procedures, especially when the data are unstructured (text-based). Drawing on literature on natural language processing, we critically examine the possibility of leveraging advances in text mining and predictive modeling computer software programs as a surrogate for human raters in a selection context. We explain how to "train" a computer program to emulate a human rater when scoring accomplishment records. We then examine the reliability of the computer's scores, provide preliminary evidence of their construct validity, demonstrate that this practice does not produce scores that disadvantage minority groups, illustrate the positive financial impact of adopting this practice in an organization (N ∼ 46,000 candidates), and discuss implementation issues. Finally, we discuss the potential implications of using computer scoring to address the adverse impact-validity dilemma. We suggest that it may provide a cost-effective means of using predictors that have comparable validity but have previously been too expensive for large-scale screening. (Psyc

  20. Abitudini alimentari di un campione di popolazione giovanile siciliana e calabrese

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    M.L. Calisto

    2003-05-01

    Full Text Available

    Obiettivo: valutare le abitudini alimentari, igieniche e la percezione di sé in un campione di studenti siciliani e calabresi.

    Materiali e Metodi: è stato condotto uno studio basato sulla compilazione in classe di un questionario, somministrato a 933 studenti dalla quarta elementare all’ultima classe di scuola superiore. Le schede con domande di tipo aperto e chiuso, a risposta singola o multipla, hanno fornito notizie di carattere antropometrico e sociofamiliare, sulle abitudini igienico alimentari e sulla percezione di sé.

    Risultati: dai dati emerge che l’ 85,6% dei ragazzi effettua la prima colazione consumando latte (43,9%, latte e caffè (30,3%, biscotti (31,8% e cereali (18,9%. L’ 89,7% fa uno spuntino a metà mattinata per lo più con panino e salumi (51,6%. Nel pomeriggio l’ 89,5% fa la merenda consumando soprattutto merendine (37,3% e succhi di frutta (28,3%.

    Circa la frequenza di assunzione di alimenti, carne, salumi, formaggi e patate sono consumati più volte a settimana; uova e pesce una volta a settimana; pasta una volta al giorno; pane e frutta fresca tutti i giorni a pranzo e a cena. Meno consumati verdura e legumi, con una preferenza per lenticchie e piselli. Il 54,6% consuma cioccolata ed il 53,4% chewing-gum. Il 78,2% consuma i pasti in compagnia dei familiari, guardando la televisione (69,8%. Dall’indagine si evince che i ragazzi non sempre hanno una visione corretta del loro corpo, infatti il 68,9% di essi, nonostante sia normopeso vorrebbe essere più magro.

    Conclusioni: si rendono necessari interventi educativi per migliorare le abitudini alimentari, favorendo la scelta di cibi sani. Occorre incrementare il consumo di verdure, legumi e frutta riducendo quello dei dolciumi che favoriscono sovrappeso e obesità. Inoltre bisogna realizzare una maggiore collaborazione tra scuola e sanità per educare i ragazzi ad una

  1. Constructive applications of composite gypsum reinforced with Typha Latifolia fibres

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    Garcia Santos, A.

    2004-03-01

    Full Text Available The present research analyses the possibility to reinforce gypsum using enea fibres (Typha Latifolia creating a compound material in wich the fibres contribute to increase mechanical resistance, producing as well a reduction of the weight and a possible regulation of the set time.

    La investigación presente analiza la posibilidad de reforzar los morteros de escayola mediante la utilización dé fibras de Typha Latifolia, creando un material compuesto en el que las fibras contribuyen al aumento de resistencia mecánica, a la vez que se produce una reducción del peso y una regulación de los tiempos de fraguado. Las propiedades de estos materiales hacen que, en determinadas aplicaciones, su utilización resulte ventajosa con respecto a materiales tradicionales.

  2. New flavonoids with cytotoxicity from the roots of Flemingia latifolia.

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    Xie, Guoyong; Lin, Binbin; Qin, Xiaoying; Wang, Guokai; Wang, Qi; Yuan, Jun; Li, Chao; Qin, Minjian

    2015-07-01

    Flemingia latifolia is a folk medicine in China, which is used for treating rheumatism, arthropathy, chronic nephritis and menopausal syndrome. The phytochemicals of the plant have seldom been studied so far. In present study, three new compounds, a flavanone quinone (flemingiquinone A) (1), a prenylated dihydroflavonoid (khonklonginol I) (2) and an isoflavonoid (flemilatifolin B) (3) were isolated from the roots of F. latifolia. Their structures were established by (1)H and (13)C NMR spectra and 2D NMR experiments, including COSY, HMQC, HMBC and ROESY. Meanwhile, the compounds were evaluated for cytotoxicity against two human cancer cell lines, SMMC-7721 and A-549. The results showed that compounds 1 and 2 possessed moderate antiproliferative effects on SMMC-7721 and A-549 cell lines. PMID:26025855

  3. Arbutin derivatives from the seeds of Madhuca latifolia.

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    Khan, Shazia; Kardar, M Nadeem; Siddiqui, Bina S

    2011-11-01

    A new arbutin derivative, madhuglucoside (1), along with three known arbutin derivatives were isolated from the seeds of Madhuca latifolia in addition to seven other known constituents. Their structures were established on the basis of spectral analysis. Compounds 1a, 2a and 3a were obtained in a pure state after acetylation of the mother fraction and characterized as their acetyl derivatives. PMID:22224283

  4. Abnormal Seedlings in Madhuca latifolia-An important biodiesel tree

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    sangram bhanudas chavan

    2014-09-01

    Full Text Available Mahua is one of the most important tree species for tribals in parts of central India for their livelihood security. It is promising oil yielding tree species for biodiesel production having properties that replaces diesel fuel. During the germination study of Madhuca latifolia conducted at NRCAF nursery, abnormalities in the form of twin and triplet seedlings were recorded. Reporting of this type of occurrence will be helpful for management of seedlings in nursery as well as for future breeding program.

  5. The SILENE reactor: an instrument suitable for studying the impact of intermediate and high radiation doses; Reacteur silene : outil adapte aux etudes appliquees a l'effet des moyennes et fortes doses

    Energy Technology Data Exchange (ETDEWEB)

    Verray, B.; Leo, Y.; Fouillaud, P. [Commissariat a l' Energie Atomique, CEA, Service de Recherches en Neutronique et Criticite, SNRC, Valduc, Is sur Tille (France)

    2002-07-01

    Designed in 1974 to study the phenomenology and consequences of a criticality accident, the SILENE experimental reactor, an intense source of mixed neutron and gamma radiation, is also suited to radiobiological studies. (author)

  6. Studies on the terpene metabolism in Lavandula latifolia Medicus

    OpenAIRE

    Mendoza Poudereux, Isabel

    2013-01-01

    Resumen en castellano Estudio del metabolismo de los terpenos en Lavandula latifolia Medicus Justificación y objetivos La biosíntesis de los dos precursores universales de los terpenos vegetales, el isopentenildifosfato (IPP) y el dimetilalildifosfato (DMAPP), es un proceso complejo en el que intervienen dos rutas metabólicas independientes (Rodríguez-Concepción y Boronat, 2002; Lange y Ahkami, 2013): La ruta del mevalonato (MVA) que opera en el citosol, retículo endoplasmático y ...

  7. Phenolic compounds from the roots of Valeriana officinalis var. latifolia

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Peng-Cheng; Ran, Xin-Hui; Luo, Huai-Rong; Liu, Yu-Qing; Zhou Jun [State Key Laboratory of Phytochemistry and Plant Resources in West China. Kunming Institute of Botany, Chinese Academy of Sciences (China); Ma, Qing-Yun; Zhao, You-Xing, E-mail: zhoujun3264@yahoo.com.cn, E-mail: zhaoyouxing@itbb.org.cn [Key Laboratory of Biology and Genetic Resources of Tropical Crops. Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology. Chinese Academy of Tropical Agriculture Sciences (China)

    2013-09-15

    A new benzofuran neolignan, dihydrodehydrodiconiferyl alcohol 9-isovalerate, along with ten known phenolic compounds, olivil, pinoresinol, 8-hydroxypinoresinol, pinorespiol, 8-hydroxy- 7-epipinoresinol, trans-p-hydroxyphenyl- propenoic acid, cis-p-hydroxyphenyl-propenoic acid, ferulic acid, isoferulic acid and isovanillin were isolated from the roots of Valeriana officinalis var. latifolia. Their structures and configurations were elucidated on the basis of spectroscopic methods. The inhibitory activity for acetylcholinesterase (AChE) and enhancing activity on nerve growth factor (NGF)-mediated neurite outgrowth in PC12 cells of dihydrodehydrodiconiferyl alcohol 9-isovaterate and olivil were evaluated. (author)

  8. Analytical evaluation of dose measurement of critical accident at SILENE (Contract research)

    CERN Document Server

    Nakamura, T; Tonoike, K

    2003-01-01

    Institute for Radioprotection and Nuclear Safety (IRSN) and the OECD Nuclear Energy Agency (NEA) jointly organized SILENE Accident Dosimetry Intercomparison Exercise to intercompare the dose measurement systems of participating countries. Each participating country carried out dose measurements in the same irradiation field, and the measurement results were mutually compared. The participated in the exercise to measure the doses of gamma rays and neutron from SILENE by using thermoluminescence dosimeters (TLD's) and an alanine dosimeter. In this examination, the derived evaluation formulae for obtaining a tissue-absorbed dose from measured value (ambient dose equivalent) of TLD for neutron. We reported the tissue-absorbed dose computed using this evaluation formula to OECD/NEA. TLD's for neutron were irradiated in the TRACY facility to verify the evaluation formulae. The results of TLD's were compared with the calculations of MCNP and measurements with alanine dose meter. We found that the ratio of the dose b...

  9. Rock cliffs hazard analysis based on remote geostructural surveys: The Campione del Garda case study (Lake Garda, Northern Italy)

    Science.gov (United States)

    Ferrero, A. M.; Migliazza, M.; Roncella, R.; Segalini, A.

    2011-02-01

    The town of Campione del Garda (located on the west coast of Lake Garda) and its access road have been historically subject to rockfall phenomena with risk for public security in several areas of the coast. This paper presents a study devoted to the determination of risk for coastal cliffs and the design of mitigation measures. Our study was based on statistical rockfall analysis performed with a commercial code and on stability analysis of rock slopes based on the key block method. Hazard from block kinematics and rock-slope failure are coupled by applying the Rockfall Hazard Assessment Procedure (RHAP). Because of the huge dimensions of the slope, its morphology and the geostructural survey were particularly complicated and demanding. For these reasons, noncontact measurement methods, based on aerial photogrammetry by helicopter, were adopted. A special software program, developed by the authors, was applied for discontinuity identification and for their orientation measurements. The potentially of aerial photogrammetic survey in rock mechanic application and its improvement in the rock mass knowledge is analysed in the article.

  10. Toxicological evaluation of mowrah (Madhuca latifolia Macbride) seed meal.

    Science.gov (United States)

    Cherian, K M; Gandhi, V M; Mulky, M J

    1996-01-01

    Mowrah (M. latifolia) seeds yield 40-50% edible fat and the meal contains saponins besides protein and high level of carbohydrates. The toxicity of the meal was evaluated as it has a potential for use in animal feedstuffs. The meal was fed to young and adult rats at levels of 10 to 40% in diet. The animals showed marked inhibition of feed intake and loss of body weight resulting in mortalities. Histopathological examination revealed a gradation of damage from slight erosion of the tip of villi of intestinal mucous membrane to complete necrosis and destruction of it, with increasing amounts of mowrah seed meal in diets. The other significant change was a severe vacuolar degeneration of kidney tubular cells. Detoxification or complete removal of the toxins is necessary for utilization of the meal as animal feedingstuff. PMID:8698410

  11. Pharmacognostic standardisation of Hilleria latifolia(Lam.) H.Walt.(Phytolaccaceae)

    Institute of Scientific and Technical Information of China (English)

    Isaac; Kingsley; Amponsah; Abraham; Yeboah; Mensah; Antonia; Otoo; Merlin; Lincoln; Kwao; Mensah; Jato; Jonathan

    2014-01-01

    Objective:To establish the pharmacognostic characters for the correct identification and quality control of Hilleria latifolia(H.latifolia),an important herb in Ghanaian folklore medicine,for the treatment of infections,pain and inflammation.Methods:The macro-morphological,qualitative and quantitative microscopic features,physicochemical and phytochemical features of the medicinally used parts of H.latifolia were evaluated using standard methods.Results:The plant has simple,alternate leaves with entire margin.The lamina is ovate to broadly lanceolate with an acuminate apex.It is hypostomatic with anomocytie stomata.The plant contains abundant prismatic crystals in all parts.Starch grains abound in the roots.The quantitative indices of the leaf and physicochemical parameters have also been established.Conclusions:The pharmacognostic features established in this study may be used as part of the pharmacopoeia! standard for the correct identification and quality control of H.latifolia.

  12. EVALUATION OF ANTI-ULCER ACTIVITY OF METHANOLIC EXTRACT OF ANOGEISSUS LATIFOLIA LEAVES IN ALBINO RATS

    OpenAIRE

    Arutla Rajender; A. Suresh; V. Suresh; G. Saker; N. Varaprasad

    2011-01-01

    Anogeissus latifolia (Roxb. ex DC.) Wall. ex Guill. & Perr. (Combretaceae) has been reported to be used in the treatment of various disorders including stomach and skin diseases. We studied the antiulcer potential of the methanolic extract in order to validate ethnobotanical claims regarding the plant use in the above-mentioned disorders. Gastroprotective potential of the methanolic extract of Anogeissus latifolia (ALE) (200 and 400 mg/kg/body weight) was studied on aspirin, and ethanol-induc...

  13. Seed germination characteristics of Phillyrea angustifolia L. and P. latifolia L. (Oleaceae), two Mediterranean shrub species having lignified endocarp

    OpenAIRE

    Sara Mira; Luciana Veiga-Barbosa; Félix Pérez-García

    2015-01-01

    The aim of this study was to determine the germination characteristics of Phillyrea angustifolia L. and P. latifolia L. seeds in order to develop an optimized propagation protocol for Phillyrea species. Seeds of P. angustifolia and P. latifolia were collected from wild plants growing in Cáceres province (CW Spain) and Andalucía (S Spain), respectively. Percentage of water uptake for P. latifolia seeds was calculated. Seeds with and without endocarp were germinated at different constant and al...

  14. Evidence for asymmetrical hybridization despite pre- and post-pollination reproductive barriers between two Silene species.

    Science.gov (United States)

    Zhang, Jin-Ju; Montgomery, Benjamin R; Huang, Shuang-Quan

    2016-01-01

    Interspecific hybridization is widespread among plants; nevertheless, pre- and post-zygotic isolating mechanisms may maintain species integrity for interfertile species in sympatry despite some gene flow. Interspecific hybridization and potential isolating barriers were evaluated between co-flowering Silene asclepiadea and Silene yunnanensis in an alpine community in southwest China. We investigated morphological and molecular (nuclear microsatellites and chloroplast gene sequence) variation in sympatric populations of S. asclepiadea and S. yunnanensis. Additionally, we analyzed pollinator behaviour and compared reproductive success between the putative hybrids and their parental species. Both the molecular and morphological data indicate that there were putative natural hybrids in the field, with S. asclepiadae the ovule parent and S. yunnanensis the pollen parent. Bumblebees were the primary visitors to S. asclepiadae and putative hybrids, while butterflies were the primary visitors to S. yunnanensis Pollen production and viability were significantly lower in putative hybrids than the parental species. The direction of hybridization is quite asymmetric from S. yunnanensis to S. asclepiadea Protandry combined with later peak flowering of S. yunnanensis, and pollinator preference may have contributed to the asymmetric pattern of hybridization, but putative hybrids were rare. Our results thus suggest that despite gene flow, S. asclepiadea and S. yunnanensis can maintain species boundaries, perhaps as a result of floral isolation and low fecundity of the hybrids. PMID:27178066

  15. Arsenate tolerance in Silene paradoxa does not rely on phytochelatin-dependent sequestration

    International Nuclear Information System (INIS)

    Arsenate tolerance, As accumulation and As-induced phytochelatin accumulation were compared in populations of Silene paradoxa, one from a mine site enriched in As, Cu and Zn, the other from an uncontaminated site. The mine population was significantly more arsenate-tolerant. Arsenate uptake and root-to-shoot transport were slightly but significantly higher in the non-mine plants. The difference in uptake was quantitatively insufficient to explain the difference in tolerance between the populations. As accumulation in the roots was similar in both populations, but the mine plants accumulated much less phytochelatins than the non-mine plants. The mean phytochelatin chain length, however, was higher in the mine population, possibly due to a constitutively lower cellular glutathione level. It is argued that the mine plants must possess an arsenic detoxification mechanism other than arsenate reduction and subsequent phytochelatin-based sequestration. This alternative mechanism might explain at least some part of the superior tolerance in the mine plants. - Neither decreased uptake nor phytochelatins seem to play a role in the As tolerance in Silene paradoxa

  16. Geometrical data generation using 'La Java de Silene' GUI for FUBLIA experiment

    Energy Technology Data Exchange (ETDEWEB)

    Stankovski, Z. [Commissariat a l' Energie Atomique et aux Energies Alternatives, Paris (France)

    2012-03-15

    The FUBILA experimental program is a common project between CEA and JNES (Japan Nuclear Energy Safety Organization) investigating the physical phenomena occurring in an advanced BWR reactor fully loaded with MOX fuel << La Java de Silene >> is an original Graphical User Interface, written in Java language, for generation of 3D arbitrarily shaped geometries for the neutron transport codes used at CEA. The interface deals simultaneously with two kinds of geometries : regular and unstructured, arranged in collections. Unstructured geometries are built visually and regular ones visually or using a test editor. Regular geometries can be composed using ten different predefined and parameterized components. This paper summarizes the principles of the software and presents some new capabilities, specifically developed for generating the FUBLIA geometries. The principal new developments concern introduction of the possibility to place cylindrical cells on the rectangular cell vertices in the rectangular grid component in order to describe the mercedes between the fuel pins. The rectangular grid component was enriched with the possibility to manage successive nesting of incomplete assemblies, those disposed on the core borders. Consistent meshing of the reflector zone bordering the core was created with the APOLLO3 mesh generator. The effectiveness of the released version of Silene is illustrated with generation and management of one particular geometrical configurations describing the FUBLIA-UGC experimental phase.

  17. Arsenate tolerance in Silene paradoxa does not rely on phytochelatin-dependent sequestration

    Energy Technology Data Exchange (ETDEWEB)

    Arnetoli, Miluscia [Section of Plant Ecology and Physiology, Department of Plant Biology, University of Florence, via Micheli 1, 50121 Firenze (Italy)], E-mail: miluscia@gmail.com; Vooijs, Riet; Bookum, Wilma ten [Institute of Molecular and Cellular Biology, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam (Netherlands); Galardi, Francesca; Gonnelli, Cristina; Gabbrielli, Roberto [Section of Plant Ecology and Physiology, Department of Plant Biology, University of Florence, via Micheli 1, 50121 Firenze (Italy); Schat, Henk; Verkleij, Jos A.C. [Institute of Molecular and Cellular Biology, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam (Netherlands)

    2008-04-15

    Arsenate tolerance, As accumulation and As-induced phytochelatin accumulation were compared in populations of Silene paradoxa, one from a mine site enriched in As, Cu and Zn, the other from an uncontaminated site. The mine population was significantly more arsenate-tolerant. Arsenate uptake and root-to-shoot transport were slightly but significantly higher in the non-mine plants. The difference in uptake was quantitatively insufficient to explain the difference in tolerance between the populations. As accumulation in the roots was similar in both populations, but the mine plants accumulated much less phytochelatins than the non-mine plants. The mean phytochelatin chain length, however, was higher in the mine population, possibly due to a constitutively lower cellular glutathione level. It is argued that the mine plants must possess an arsenic detoxification mechanism other than arsenate reduction and subsequent phytochelatin-based sequestration. This alternative mechanism might explain at least some part of the superior tolerance in the mine plants. - Neither decreased uptake nor phytochelatins seem to play a role in the As tolerance in Silene paradoxa.

  18. Reproductive toxicology and nutritional studies on mahua oil (Madhuca latifolia).

    Science.gov (United States)

    Rukmini, C

    1990-09-01

    Mahua (Madhuca latifolia, Sapotaceae) seed oil is a common ingredient of hydrogenated fat in India. It is obtained from the seed kernels (which contain 45% oil, w/w), and is a pale yellow, semi-solid fat at room temperature. It has palmitic (19%), stearic (15%), oleic (42%) and linoleic (24%) as a major fatty acids. The physicochemical parameters of the oil are similar to those of other edible oils. The unsaponifiable fraction contains several unidentified polyenes. Nutritional studies were carried out in groups of 15 weanling albino rats of each sex, fed for 14 wk on a 20% protein diet, adequate in all vitamins and minerals, containing 10% mahua oil. A similar (control) group was simultaneously maintained on the same basic diet containing 10% groundnut oil. The growth, fat absorption, and retention of nitrogen, calcium and phosphorus were similar in the two groups, as were the lipid profiles of the serum, liver and heart. Multigeneration reproduction studies in groups of 15 rats of each sex, fed a diet containing 10% mahua oil, as used in the above study, indicated poor reproductive performance in the second generation. All the male rats became sterile. Histological studies indicated bilateral testicular atrophy with degenerative changes in the seminiferous tubules in the affected animals. On withdrawal of mahua oil from the diet and rehabilitation, the male animals regained their fertility. These results indicate temporary male sterility on feeding mahua oil to rats. No other adverse toxicological effects were found. PMID:2272556

  19. Arsenate tolerance in Silene paradoxa does not rely on phytochelatin-dependent sequestration.

    Science.gov (United States)

    Arnetoli, Miluscia; Vooijs, Riet; ten Bookum, Wilma; Galardi, Francesca; Gonnelli, Cristina; Gabbrielli, Roberto; Schat, Henk; Verkleij, Jos A C

    2008-04-01

    Arsenate tolerance, As accumulation and As-induced phytochelatin accumulation were compared in populations of Silene paradoxa, one from a mine site enriched in As, Cu and Zn, the other from an uncontaminated site. The mine population was significantly more arsenate-tolerant. Arsenate uptake and root-to-shoot transport were slightly but significantly higher in the non-mine plants. The difference in uptake was quantitatively insufficient to explain the difference in tolerance between the populations. As accumulation in the roots was similar in both populations, but the mine plants accumulated much less phytochelatins than the non-mine plants. The mean phytochelatin chain length, however, was higher in the mine population, possibly due to a constitutively lower cellular glutathione level. It is argued that the mine plants must possess an arsenic detoxification mechanism other than arsenate reduction and subsequent phytochelatin-based sequestration. This alternative mechanism might explain at least some part of the superior tolerance in the mine plants.

  20. A new triterpenoid, madhunolic acid, from the seeds of Madhuca latifolia.

    Science.gov (United States)

    Siddiqui, B S; Khan, S; Kardar, M N; Perwaiz, S

    2007-01-01

    A new triterpenoid, madhunolic acid, along with the three known constituents stigmasterol, 4-hydroxymethyl benzoate, and hydroquinone were isolated from the fruit seeds of Madhuca latifolia. The structure of the new compound was elucidated as 2beta,3beta,23-trihydroxyurs-5,12,20-trien-28-oic acid (1) on the basis of spectral data and chemical evidence. PMID:17566924

  1. A host plant genome (Zizania latifolia) after a century-long endophyte infection.

    Science.gov (United States)

    Guo, Longbiao; Qiu, Jie; Han, Zujing; Ye, Zihong; Chen, Chao; Liu, Chuanjun; Xin, Xiufang; Ye, Chu-Yu; Wang, Ying-Ying; Xie, Hongqing; Wang, Yu; Bao, Jiandong; Tang, She; Xu, Jie; Gui, Yijie; Fu, Fei; Wang, Weidi; Zhang, Xingchen; Zhu, Qianhua; Guang, Xuanmin; Wang, Chongzhi; Cui, Haifeng; Cai, Daguang; Ge, Song; Tuskan, Gerald A; Yang, Xiaohan; Qian, Qian; He, Sheng Yang; Wang, Jun; Zhou, Xue-Ping; Fan, Longjiang

    2015-08-01

    Despite the importance of host-microbe interactions in natural ecosystems, agriculture and medicine, the impact of long-term (especially decades or longer) microbial colonization on the dynamics of host genomes is not well understood. The vegetable crop 'Jiaobai' with enlarged edible stems was domesticated from wild Zizania latifolia (Oryzeae) approximately 2000 years ago as a result of persistent infection by a fungal endophyte, Ustilago esculenta. Asexual propagation via infected rhizomes is the only means of Jiaobai production, and the Z. latifolia-endophyte complex has been maintained continuously for two centuries. Here, genomic analysis revealed that cultivated Z. latifolia has a significantly smaller repertoire of immune receptors compared with wild Z. latifolia. There are widespread gene losses/mutations and expression changes in the plant-pathogen interaction pathway in Jiaobai. These results show that continuous long-standing endophyte association can have a major effect on the evolution of the structural and transcriptomic components of the host genome. PMID:26072920

  2. Nauclea latifolia aqueous leaf extract eliminates hepatic and cerebral Plasmodium berghei parasite in experimental mice

    Institute of Scientific and Technical Information of China (English)

    Innocent; Onyesom; Ejovi; Osioma; Precious; Chiamaka; Okereke

    2015-01-01

    Objective: To assess the effects of hot water leaf extract of Nauclea latifolia(N. latifolia) on antioxidant status, lipid peroxidation values and parasite levels in hepatic and brain tissue of experimental mice(BALB/c) infected with Plasmodium berghei(P. berghei) malaria.Methods: Forty nine mice were divided into seven groups(n = 7) and used for the study. Group A(control) were given 0.2 m L/kg phosphate buffer saline; Group B mice were infected with P. berghei and treated with phosphate buffer saline. Groups C and D mice were also infected but treated with 200 and 300 mg/kg body weight of leaf extract respectively. Groups E and F mice were not infected, but received 200 and 300 mg/kg of leaf extract respectively. Group G mice were infected and treated with chloroquine(5 mg/kg). Liver and brain tissues of mice were prepared for both biochemical assay and microscopic examination. Results: Results showed that P. berghei malaria infection induced oxidative stress in both liver and brain tissues as evidenced by the significant(P < 0.05) decrease in antioxidants: superoxide dismutase, reduced glutathione and catalase. These reductions perhaps caused compromise in membrane integrity as indicated by the significant increase in lipid peroxidation product malondialdhyde. Malaria parasites were also identified in these tissues. However, N. latifolia treatment eliminated the parasites in tissues and protected them from oxidative damage even better than chloroquine treatment did, whose anti-malarial potency also cleared tissue parasites. The measurement of protection by N. latifolia against damage was strengthened by the insignificant micro structural alterations.Conclusions: The bioactive phytochemical(s) in N. latifolia should be structured and the mechanism(s) of its antimalarial tendency should be further investigated.

  3. Comparative high-throughput transcriptome sequencing and development of SiESTa, the Silene EST annotation database

    Directory of Open Access Journals (Sweden)

    Marais Gabriel AB

    2011-07-01

    Full Text Available Abstract Background The genus Silene is widely used as a model system for addressing ecological and evolutionary questions in plants, but advances in using the genus as a model system are impeded by the lack of available resources for studying its genome. Massively parallel sequencing cDNA has recently developed into an efficient method for characterizing the transcriptomes of non-model organisms, generating massive amounts of data that enable the study of multiple species in a comparative framework. The sequences generated provide an excellent resource for identifying expressed genes, characterizing functional variation and developing molecular markers, thereby laying the foundations for future studies on gene sequence and gene expression divergence. Here, we report the results of a comparative transcriptome sequencing study of eight individuals representing four Silene and one Dianthus species as outgroup. All sequences and annotations have been deposited in a newly developed and publicly available database called SiESTa, the Silene EST annotation database. Results A total of 1,041,122 EST reads were generated in two runs on a Roche GS-FLX 454 pyrosequencing platform. EST reads were analyzed separately for all eight individuals sequenced and were assembled into contigs using TGICL. These were annotated with results from BLASTX searches and Gene Ontology (GO terms, and thousands of single-nucleotide polymorphisms (SNPs were characterized. Unassembled reads were kept as singletons and together with the contigs contributed to the unigenes characterized in each individual. The high quality of unigenes is evidenced by the proportion (49% that have significant hits in similarity searches with the A. thaliana proteome. The SiESTa database is accessible at http://www.siesta.ethz.ch. Conclusion The sequence collections established in the present study provide an important genomic resource for four Silene and one Dianthus species and will help to

  4. Phenotypic and genotypic components of growth and reproduction in Typha latifolia: experimental studies in three contrasting marshes

    Energy Technology Data Exchange (ETDEWEB)

    Grace, J.B.

    1980-01-01

    The magnitude and causes of intraspecific variation in biomass production and allocation, and morphology for Typha latifolia L. from three marshes which can be distinguished by their successional maturity were investigated. The first stage of investigation was to determine the environmental characteristics of the three marshes and the characteristics of the T. latifolia populations. Second, in situ studies of /sup 14/C fixation and allocation were used to determine the phenotypic variation in biomass production and allocation. Third, populations were sampled for genotypic variation in biomass allocation patterns by comparing growth in controlled garden experiments. Fourth, the growth of different biotypes was compared by transplantation into natural stands of T. latifolia. And fifth, the intraspecific variations were considered in terms of their consequences for the persistence of T. latifolia in habitats over successional time.

  5. The effects of stress intensity and stress type on inbreeding depression in Silene vulgaris.

    Science.gov (United States)

    Sandner, Tobias Michael; Matthies, Diethart

    2016-06-01

    Inbreeding depression (ID) is generally assumed to increase under stressful conditions, but a number of studies have found the opposite pattern, that is that crossed offspring were more capable of exploiting benign conditions. Alternatively, the phenotypic variation hypothesis predicts that not stress intensity, but enhanced phenotypic variation in an environment leads to increased ID. We subjected inbred and crossed offspring of Silene vulgaris to drought, simulated herbivory, copper contamination, and two levels of nutrient deficiency and shade. In contrast to the predominant expectation, most stress treatments decreased inbreeding depression. With increasing nutrient limitation, ID decreased strongly, whereas under increasing shade ID did not change. These differences may be due to purging in the population of origin where conditions are nutrient-poor and dry, but not shaded. In contrast to the greenhouse experiment, ID was higher in a field site than in a more benign common garden. However, the predictions of the phenotypic variation hypothesis were met in both the greenhouse and the field versus garden experiment. The results suggest that there may be no general relationship between ID and stress intensity, but specific effects of stress type and the novelty and variability of the environment. PMID:27110935

  6. A taxonomic revision of the southern African native and naturalized species of Silene L. (Caryophyllaceae

    Directory of Open Access Journals (Sweden)

    J. C. Manning

    2012-12-01

    Full Text Available The native and naturalized species of Silene L. in southern Africa are reviewed, with full synonomy and the description of two new species from the West Coast of Western Cape. Eight native species and three naturalized species are recognized, including the first identification in southern Africa of the Mediterranean S. nocturna L. The identity of S. aethiopica Burm., which has remained unknown since its description, is established and is found to be the oldest name for S. clandestina Jacq. Patterns of morphological variation within each species are discussed and subspecies are recognized for geographically segregated groups of populations that are ± morphologically diagnosable. The following new names or combinations are made among the southern African taxa: S. aethiopica subsp. longiflora; S. burchellii subsp. modesta, subsp. multiflora, and subsp. pilosellifolia; S. crassifolia subsp. primuliflora; S. saldanhensis; S. rigens; and S. undulata subsp. polyantha. Each taxon is described, with information on ecology and distribution, and most species are illustrated, including SEM micrographs of the seeds.

  7. Mosaic origins of a complex chimeric mitochondrial gene in Silene vulgaris.

    Directory of Open Access Journals (Sweden)

    Helena Storchova

    Full Text Available Chimeric genes are significant sources of evolutionary innovation that are normally created when portions of two or more protein coding regions fuse to form a new open reading frame. In plant mitochondria astonishingly high numbers of different novel chimeric genes have been reported, where they are generated through processes of rearrangement and recombination. Nonetheless, because most studies do not find or report nucleotide variation within the same chimeric gene, evolution after the origination of these chimeric genes remains unstudied. Here we identify two alleles of a complex chimera in Silene vulgaris that are divergent in nucleotide sequence, genomic position relative to other mitochondrial genes, and expression patterns. Structural patterns suggest a history partially influenced by gene conversion between the chimeric gene and functional copies of subunit 1 of the mitochondrial ATP synthase gene (atp1. We identified small repeat structures within the chimeras that are likely recombination sites allowing generation of the chimera. These results establish the potential for chimeric gene divergence in different plant mitochondrial lineages within the same species. This result contrasts with the absence of diversity within mitochondrial chimeras found in crop species.

  8. Geometrical data generation using 'La Java de Silene' GUI for FUBLIA experiment

    International Nuclear Information System (INIS)

    The FUBILA experimental program is a common project between CEA and JNES (Japan Nuclear Energy Safety Organization) investigating the physical phenomena occurring in an advanced BWR reactor fully loaded with MOX fuel > is an original Graphical User Interface, written in Java language, for generation of 3D arbitrarily shaped geometries for the neutron transport codes used at CEA. The interface deals simultaneously with two kinds of geometries : regular and unstructured, arranged in collections. Unstructured geometries are built visually and regular ones visually or using a test editor. Regular geometries can be composed using ten different predefined and parameterized components. This paper summarizes the principles of the software and presents some new capabilities, specifically developed for generating the FUBLIA geometries. The principal new developments concern introduction of the possibility to place cylindrical cells on the rectangular cell vertices in the rectangular grid component in order to describe the mercedes between the fuel pins. The rectangular grid component was enriched with the possibility to manage successive nesting of incomplete assemblies, those disposed on the core borders. Consistent meshing of the reflector zone bordering the core was created with the APOLLO3 mesh generator. The effectiveness of the released version of Silene is illustrated with generation and management of one particular geometrical configurations describing the FUBLIA-UGC experimental phase

  9. EVALUATION OF ANTI-ULCER ACTIVITY OF METHANOLIC EXTRACT OF ANOGEISSUS LATIFOLIA LEAVES IN ALBINO RATS

    Directory of Open Access Journals (Sweden)

    Arutla Rajender

    2011-02-01

    Full Text Available Anogeissus latifolia (Roxb. ex DC. Wall. ex Guill. & Perr. (Combretaceae has been reported to be used in the treatment of various disorders including stomach and skin diseases. We studied the antiulcer potential of the methanolic extract in order to validate ethnobotanical claims regarding the plant use in the above-mentioned disorders. Gastroprotective potential of the methanolic extract of Anogeissus latifolia (ALE (200 and 400 mg/kg/body weight was studied on aspirin, and ethanol-induced ulcers. The results of the present study showed for the first time that the ALE possessed gastroprotective activity as evidenced by its significant inhibition in the formation of ulcers induced by chemical agents with a maximum of 88.95%. The parameters studied were ulcer index, gastric juice volume, pH, free acidity and total acidity. These findings could justify, at least partially, the inclusion of this plant in the management of gastric disorders in traditional medicine.

  10. Antibacterial and antioxidant activities of hydroalcoholic stem bark extract ofSchotia latifolia Jacq

    Institute of Scientific and Technical Information of China (English)

    Sunday Oyewole Oyedemi; Anthony Jide Afolayan

    2011-01-01

    Objective:To investigate the antibacterial and antioxidant activities of hydroalcoholic extract ofSchotia latifolia (S. latifolia) bark commonly used in South Africa traditional medicine for the treatment of various ailments.Methods: The antibacterial test andMIC was determined by using agar well diffusion and dilution methods respectively against eight strains of bacteria. The total phenol, proanthocyanidin and flavonoid contents ofS. latifolia were assessed using standard methods. The antioxidant activity of the extract was evaluated using ferric reducing power and the free radical scavenging activity against1,1-diphenyl-2-picrylhydrazyl(DPPH), 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic-acid(ABTS), nitric oxide(NO), hydrogen peroxide(H2O2)and lipid oxidation(LO).Results:The antibacterial activity demonstrated an appreciable effect against all the gram positive bacteria atMIC between0.016 and10 mg/mL while that of gram negative bacteria was above10 mg/mL. The plant extract exhibited high concentration of proanthocyanidin [(300.00±0.10) mgCE/g], followed by flavonoid [(12.46±0.04 mg) TE/g] and phenol [(11.06±0.03) mgQE/g] contents. Similarly, the extract at0.5 mg/mL scavenges DPPH, ABTS, H2O2, LO andNO by87.55%, 89.47%, 77.15%, 86.48% and77.75% of the radicals respectively. The reducing power was also found to be concentration dependent.Conclusions:Our data suggest thatS. latifolia extract has antibacterial and antioxidants activity and thus could be used as alternative therapy against antibiotic resistance bacteria and to prevent many radical related diseases.

  11. Efficiency of Phragmites australis and Typha latifolia for heavy metal removal from wastewater.

    Science.gov (United States)

    Kumari, Menka; Tripathi, B D

    2015-02-01

    A cost-effective and promising technology has been demonstrated for the removal of copper (Cu), cadmium (Cd), chromium (Cr), nickel (Ni), iron (Fe), lead (Pb) and zinc (Zn) from urban sewage mixed with industrial effluents within 14 days. With the help of P. australis and T. latifolia grown alone and in combination batch experiments were designed to assess the removal of heavy metals from the wastewater collected from 5 sampling stations. The results revealed that P. australis performed better than T. latifolia for Cu, Cd, Cr, Ni, Fe, Pb and Zn removal, while mixing of the plant species further enhanced the removal of Cu to 78.0±1.2%, Cd to 60.0±1.2%, Cr to 68.1±0.4%, Ni to 73.8±0.6%, Fe to 80.1±0.3%, Pb to 61.0±1.2% and Zn to 61.0±1.2% for wastewater samples from Raj Ghat. Negative correlation coefficients of Cu, Cd, Cr, Ni, Fe, Pb and Zn concentrations in wastewater with the retention time revealed that there was an increase in the heavy metal removal rate with retention time. P. australis showed higher accumulative capacities for Cu, Cd, Cr, Ni and Fe than T. latifolia. P. australis and T. latifolia grown in combination can be used for the removal of Cu, Cd, Cr, Ni, Fe, Pb and Zn from the urban sewage mixed with industrial effluents within 14 days.

  12. Nauclea latifolia aqueous leaf extract eliminates hepatic and cerebral Plasmodium berghei parasite in experimental mice

    Institute of Scientific and Technical Information of China (English)

    Innocent Onyesom; Ejovi Osioma; Precious Chiamaka Okereke

    2015-01-01

    Objective:To assess the effects of hot water leaf extract of Nauclea latifolia (N. latifolia) on antioxidant status, lipid peroxidation values and parasite levels in hepatic and brain tissue of experimental mice (BALB/c) infected with Plasmodium berghei (P. berghei) malaria. Methods:Forty nine mice were divided into seven groups (n=7) and used for the study. Group A (control) were given 0.2 mL/kg phosphate buffer saline;Group B mice were infected with P. berghei and treated with phosphate buffer saline. Groups C and D mice were also infected but treated with 200 and 300 mg/kg body weight of leaf extract respectively. Groups E and F mice were not infected, but received 200 and 300 mg/kg of leaf extract respectively. Group G mice were infected and treated with chloroquine (5 mg/kg). Liver and brain tissues of mice were prepared for both biochemical assay and microscopic examination. Results:Results showed that P. berghei malaria infection induced oxidative stress in both liver and brain tissues as evidenced by the significant (P Conclusions:The bioactive phytochemical(s) in N. latifolia should be structured and the mechanism(s) of its antimalarial tendency should be further investigated.

  13. Comparative mapping of the wild perennial Glycine latifolia and soybean (G. max) reveals extensive chromosome rearrangements in the genus Glycine.

    Science.gov (United States)

    Chang, Sungyul; Thurber, Carrie S; Brown, Patrick J; Hartman, Glen L; Lambert, Kris N; Domier, Leslie L

    2014-01-01

    Soybean (Glycine max L. Mer.), like many cultivated crops, has a relatively narrow genetic base and lacks diversity for some economically important traits. Glycine latifolia (Benth.) Newell & Hymowitz, one of the 26 perennial wild Glycine species related to soybean in the subgenus Glycine Willd., shows high levels of resistance to multiple soybean pathogens and pests including Alfalfa mosaic virus, Heterodera glycines Ichinohe and Sclerotinia sclerotiorum (Lib.) de Bary. However, limited information is available on the genomes of these perennial Glycine species. To generate molecular resources for gene mapping and identification, high-density linkage maps were constructed for G. latifolia using single nucleotide polymorphism (SNP) markers generated by genotyping by sequencing and evaluated in an F2 population and confirmed in an F5 population. In each population, greater than 2,300 SNP markers were selected for analysis and segregated to form 20 large linkage groups. Marker orders were similar in the F2 and F5 populations. The relationships between G. latifolia linkage groups and G. max and common bean (Phaseolus vulgaris L.) chromosomes were examined by aligning SNP-containing sequences from G. latifolia to the genome sequences of G. max and P. vulgaris. Twelve of the 20 G. latifolia linkage groups were nearly collinear with G. max chromosomes. The remaining eight G. latifolia linkage groups appeared to be products of multiple interchromosomal translocations relative to G. max. Large syntenic blocks also were observed between G. latifolia and P. vulgaris. These experiments are the first to compare genome organizations among annual and perennial Glycine species and common bean. The development of molecular resources for species closely related to G. max provides information into the evolution of genomes within the genus Glycine and tools to identify genes within perennial wild relatives of cultivated soybean that could be beneficial to soybean production. PMID

  14. Comparative mapping of the wild perennial Glycine latifolia and soybean (G. max reveals extensive chromosome rearrangements in the genus Glycine.

    Directory of Open Access Journals (Sweden)

    Sungyul Chang

    Full Text Available Soybean (Glycine max L. Mer., like many cultivated crops, has a relatively narrow genetic base and lacks diversity for some economically important traits. Glycine latifolia (Benth. Newell & Hymowitz, one of the 26 perennial wild Glycine species related to soybean in the subgenus Glycine Willd., shows high levels of resistance to multiple soybean pathogens and pests including Alfalfa mosaic virus, Heterodera glycines Ichinohe and Sclerotinia sclerotiorum (Lib. de Bary. However, limited information is available on the genomes of these perennial Glycine species. To generate molecular resources for gene mapping and identification, high-density linkage maps were constructed for G. latifolia using single nucleotide polymorphism (SNP markers generated by genotyping by sequencing and evaluated in an F2 population and confirmed in an F5 population. In each population, greater than 2,300 SNP markers were selected for analysis and segregated to form 20 large linkage groups. Marker orders were similar in the F2 and F5 populations. The relationships between G. latifolia linkage groups and G. max and common bean (Phaseolus vulgaris L. chromosomes were examined by aligning SNP-containing sequences from G. latifolia to the genome sequences of G. max and P. vulgaris. Twelve of the 20 G. latifolia linkage groups were nearly collinear with G. max chromosomes. The remaining eight G. latifolia linkage groups appeared to be products of multiple interchromosomal translocations relative to G. max. Large syntenic blocks also were observed between G. latifolia and P. vulgaris. These experiments are the first to compare genome organizations among annual and perennial Glycine species and common bean. The development of molecular resources for species closely related to G. max provides information into the evolution of genomes within the genus Glycine and tools to identify genes within perennial wild relatives of cultivated soybean that could be beneficial to soybean

  15. Investigating past range dynamics for a weed of cultivation, Silene vulgaris.

    Science.gov (United States)

    Sebasky, Megan E; Keller, Stephen R; Taylor, Douglas R

    2016-07-01

    Since the last glacial maximum (LGM), many plant and animal taxa have expanded their ranges by migration from glacial refugia. Weeds of cultivation may have followed this trend or spread globally following the expansion of agriculture or ruderal habitats associated with human-mediated disturbance. We tested whether the range expansion of the weed Silene vulgaris across Europe fit the classical model of postglacial expansion from southern refugia, or followed known routes of the expansion of human agricultural practices. We used species distribution modeling to predict spatial patterns of postglacial expansion and contrasted these with the patterns of human agricultural expansion. A population genetic analysis using microsatellite loci was then used to test which scenario was better supported by spatial patterns of genetic diversity and structure. Genetic diversity was highest in southern Europe and declined with increasing latitude. Locations of ancestral demes from genetic cluster analysis were consistent with areas of predicted refugia. Species distribution models showed the most suitable habitat in the LGM on the southern coasts of Europe. These results support the typical postglacial northward colonization from southern refugia while refuting the east-to-west agricultural spread as the main mode of expansion for S. vulgaris. We know that S. vulgaris has recently colonized many regions (including North America and other continents) through human-mediated dispersal, but there is no evidence for a direct link between the Neolithic expansion of agriculture and current patterns of genetic diversity of S. vulgaris in Europe. Therefore, the history of range expansion of S. vulgaris likely began with postglacial expansion after the LGM, followed by more recent global dispersal by humans. PMID:27547314

  16. Transcriptome and biochemical analysis of a flower color polymorphism in Silene littorea (Caryophyllaceae

    Directory of Open Access Journals (Sweden)

    Inés eCasimiro-Soriguer

    2016-02-01

    Full Text Available Flower color polymorphisms are widely used as model traits from genetics to ecology, yet determining the biochemical and molecular basis can be challenging. Anthocyanin-based flower color variations can be caused by at least 12 structural and three regulatory genes in the anthocyanin biosynthetic pathway. We use mRNA-Seq to simultaneously sequence and estimate expression of these candidate genes in nine samples of Silene littorea representing three color morphs (dark pink, light pink and white across three developmental stages in hopes of identifying the cause of flower color variation. We identified 29 putative paralogues for the 15 candidate genes in the anthocyanin biosynthetic pathway. We assembled complete coding sequences for 16 structural loci and nine of ten regulatory loci. Among these 29 putative paralogues, we identified 622 SNPs, yet only nine synonymous SNPs in Ans had allele frequencies that differentiated pigmented petals (dark pink and light pink from white petals. These Ans allele frequency differences were further investigated with an expanded sequencing survey of 38 individuals, yet no SNPs consistently differentiated the color morphs. We also found one locus, F3h1, with strong differential expression between pigmented and white samples (>42x. This may be caused by decreased expression of Myb1a in white petal buds. Myb1a in S. littorea is a regulatory locus closely related to Subgroup 7 Mybs known to regulate F3h and other loci in the first half of the ABP in model species. We then compare the mRNA-Seq results with petal biochemistry which revealed cyanidin as the primary anthocyanin and five flavonoid intermediates. Concentrations of three of the flavonoid intermediates were significantly lower in white petals than in pigmented petals (rutin, quercetin and isovitexin. The biochemistry results for rutin, quercetin, luteolin and apigenin are consistent with the transcriptome results suggesting a blockage at F3h, possibly caused

  17. The 2″-O-glucosylation of vitexin and isovitexin in petals of Silene alba is catalysed by two different enzymes

    NARCIS (Netherlands)

    Heinsbroek, R.; Brederode, J. van; Nigtevecht, G. van; Maas, J.; Kamsteeg, J.; Besson, E.; Chopin, J.

    1980-01-01

    Two separate genes, Fg and Vg, which govern the presence of isovitexin 2″-O-glucoside and vitexin 2″-O-glucoside respectively in the petals of Silene alba control different glucosyltransferases. In Vg/Vg,fg/fg plants no isovitexin 2″-O-glucosyltransferase was present and in vg/vg,Fg/Fg plants no vit

  18. Genetical and biochemical evidence that the hydroxylation pattern of the anthocyanin B-ring in Silene dioica is determined at the p-coumaroyl-coenzyme a stage

    NARCIS (Netherlands)

    Kamsteeg, J.; Brederode, J. van; Nigtevecht, G. van

    1980-01-01

    In petals of Silene dioica, gene P controls the 3′-hydroxylation of the anthocyanin B-ring and the hydroxylation pattern of the hydroxycinnamoyl acyl group bound to the 4″'-hydroxyl group of rhamnose of anthocyanidin 3-rhamnosyl(1→6)glucoside-5-glucoside. In this paper, experiments are presented whi

  19. Glyphosate associated with other herbicides for control of Commelina benghalensis and Spermacoce latifoliaGlyphosate associado a outros herbicidas no controle de Commelina benghalensis and Spermacoce latifolia

    Directory of Open Access Journals (Sweden)

    Diego Gonçalves Alonso

    2011-08-01

    Full Text Available The widespread cultivation of Roundup Ready soybeans enabled the use of glyphosate for post-emergence weed control in, turning weed handling into an easier and less costly operation. Despite such improvements, controlling glyphosate tolerant species can still be improved by associations with other herbicides. The aimed at evaluating the effect of the association of different herbicides with glyphosate in relation to Commelina benghalensis and Spermacoce latifolia control, after spraying plants in two different growth stages (1-3 and 4-6 leaves. For that, four experiments were carried out under greenhouse conditions, where glyphosate was applies at rates of 480 and 960 g a.e. ha-1, isolated or combined in tank mixtures with cloransulam-methyl (30,24 g ha-1, chlorimuron-ethyl (12,5 g ha-1, imazethapyr (80 g ha-1, fomesafen (62,5 g ha-1, lactofen (72 g ha-1, flumiclorac-pentyl (30 g ha-1 and bentazon (480 g ha-1, besides a no-herbicide check. We evaluated the percentage of control at 3, 7, 14, 21, 28 and 35 days after application (DAA and shoot dry mass (35 DAA. For C. benghalensis control, when herbicides were applied at 1-3 leaves stage, all treatments performed satisfactorily in weed control. For S. latifolia, the highest levels of weed control when applications were made at 4-6 leaves stage, when even glyphosate alone at lowest rate (480 g a.e. ha-1, provided excellent weed control. Overall, the use of tank mixtures improved weed control of both species when the rate of 480 g a.e. ha-1 of glyphosate was applied at earlier stages of weed development.A liberação da soja transgênica possibilitou o uso do glyphosate para o controle das plantas daninhas em pós-emergência, facilitando o manejo e diminuindo os custos. No entanto, o controle de algumas espécies tolerantes ao glyphosate pode ser otimizado com associações com outros herbicidas. O trabalho teve como objetivo verificar o efeito da associação de diferentes herbicidas com

  20. Distribution characteristics of ammonia-oxidizing bacteria in the Typha latifolia constructed wetlands using fluorescent in situ hybridization (FISH).

    Science.gov (United States)

    Yan, Li; Inamori, Ryuhei; Gui, Ping; Xu, Kai-qin; Kong, Hai-nan; Matsumura, Masatoshi; Inamori, Yuhei

    2005-01-01

    A molecular biology method, fluorescent in situ hybridization (FISH), in which the pre-treatment was improved in allusion to the media of the constructed wetlands (CW), e.g. the soil and the grit, was used to investigate the vertical distribution characteristics of ammonia-oxidizing bacteria (AOB) quantity and the relation with oxidation-reduction potential (ORP) in the Typha latifolia constructed wetlands under three different loadings in summer from May to September. Results showed that the quantity of the AOB decreased in the Typha latifolia CW with the increase of vertical depth. However, the AOB quantity was 2-4 times the quantity of the control in the root area. Additionally, ORP in the rhizosphere was found to be higher than other areas, which showed that Typha latifolia CW was in an aerobic state in summer when using simulated non-point sewage at the rural area of Taihu Lake in China and small town combined sewage.

  1. Indole Alkaloids of Alstonia angustifolia var. latifolia as Green Inhibitor for Mild Steel Corrosion in 1 M HCl Media

    Science.gov (United States)

    Raja, Pandian Bothi; Qureshi, Ahmad Kaleem; Rahim, Afidah Abdul; Awang, Khalijah; Mukhtar, Mat Ropi; Osman, Hasnah

    2013-04-01

    The inhibition effect of mild steel (MS) corrosion in 1 M HCl was studied by the addition of indole alkaloids (crude) isolated from Alstonia angustifolia var. latifolia ( A. latifolia) leaves at 303 K. Potentiodynamic polarization, impedance spectroscopy, scanning electron microscopy (SEM), and Fourier transform infrared (FTIR) analyses were used for this study. Results show that the isolated alkaloid extract of A. latifolia is a good inhibitor and exhibited maximum inhibition efficiency (above 80%) at concentrations between 3 and 5 mg/L. Polarization measurements indicated that the inhibitor does not alter the mechanism of either anodic or cathodic reactions and acted as mixed-type inhibitor. The inhibition efficiencies of both electrochemical techniques are found to be in good agreement and adsorption of inhibitor follows Langmuir isotherm. Adsorption of inhibitor over metal surface was well supported by the SEM studies, while FTIR studies evidenced the presence of indole alkaloids as green inhibitor that reduces the rate of corrosion.

  2. Ilex latifolia Prevents Amyloid β Protein (25-35)-Induced Memory Impairment by Inhibiting Apoptosis and Tau Phosphorylation in Mice.

    Science.gov (United States)

    Kim, Joo Youn; Lee, Hong Kyu; Jang, Ji Yeon; Yoo, Jae Kuk; Seong, Yeon Hee

    2015-12-01

    Ilex latifolia Thunb. (Aquifoliaceae), a Chinese bitter tea called "kudingcha," has been widely consumed as a health beverage and found to possess antioxidant, antidiabetic, antihypertensive, anti-inflammatory, and anti-ischemic activities. The aim of the present study was to investigate the neuroprotective effects of an ethanol extract of I. latifolia against amyloid β protein (Aβ)-induced memory impairment in mice and neurotoxicity in cultured rat cortical neurons. Memory impairment in mice was induced by intracerebroventricular injection of 15 nmol Aβ (25-35) and measured by the passive avoidance test and Morris water maze test. Chronic administration of I. latifolia (25-100 mg/kg, p.o.) significantly prevented Aβ (25-35)-induced memory loss. I. latifolia also prevented the decrease of glutathione concentrations, increased lipid peroxidation, expression of phosphorylated tau (p-tau), and changes in apoptosis-associated proteins in the memory-impaired mouse brain. Exposure of cultured cortical neurons to 10 μM Aβ (25-35) for 36 h induced neuronal apoptotic death. The neuronal cell death, elevation of intracellular Ca(2+) concentration, generation of reactive oxygen species, and expression of proapoptotic proteins caused by Aβ (25-35) in the cultured neurons were inhibited by treatment with I. latifolia (1-50 μg/mL). These results suggest that I. latifolia may have a possible therapeutic role in managing cognitive impairment associated with Alzheimer's disease. The underlying mechanism might involve the antiapoptotic effects mediated by antioxidant activity and inhibition of p-tau formation.

  3. Micropropagation of Madhuca longifolia (Koenig) MacBride var. latifolia Roxb.

    Science.gov (United States)

    Rout, G R; Das, P

    1993-07-01

    Bud break and multiple shoots were induced in apical and axillary meristems derived from 10-d old seedlings of Madhuca longifolia var. latifolia on Murashige and Skoog (MS) medium supplemented with 1.0 mg/l N(6)-benzyladenine (BA) singly or in combinatiobn with 1-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA). Excised shoots were rooted on half-strength MS with IBA (1.0 mg/l) after 18d of culture. Regenerated plantlets were acclimatized and successfully transferred to soil. PMID:24196112

  4. Micropropagation of Madhuca longifolia (Koenig) MacBride var. latifolia Roxb.

    Science.gov (United States)

    Rout, G R; Das, P

    1993-07-01

    Bud break and multiple shoots were induced in apical and axillary meristems derived from 10-d old seedlings of Madhuca longifolia var. latifolia on Murashige and Skoog (MS) medium supplemented with 1.0 mg/l N(6)-benzyladenine (BA) singly or in combinatiobn with 1-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA). Excised shoots were rooted on half-strength MS with IBA (1.0 mg/l) after 18d of culture. Regenerated plantlets were acclimatized and successfully transferred to soil.

  5. Antibacterial and Antifungal Activities of Lectin Extracted from Fruiting Bodies of the Korean Cauliflower Medicinal Mushroom, Sparassis latifolia (Agaricomycetes).

    Science.gov (United States)

    Chandrasekaran, Gayathri; Lee, Young-Chul; Park, Hyun; Wu, Yuanzheng; Shin, Hyun-Jae

    2016-01-01

    In this article we describe the isolation and characterization of a novel lectin from fruiting bodies of the mushroom Sparassis latifolia. The antibacterial activity of the purified lectin against Escherichia coli and resistant strains of Staphylococcus aureus and Pseudomonas aeruginosa as well as the antifungal activity against Candida and Fusarium species were determined. Circular dichroism spectroscopy and the tryptophan blue shift assay indicated that the lectin interacts with microbial surfaces. This suggests the potential of the lectin isolated from S. latifolia, a valuable source of bioactive constituents, as a therapeutic in pharmaceutical agent. PMID:27481295

  6. An Efficient In Vitro Plantlet Regeneration from Shoot Tip Cultures of Curculigo latifolia, a Medicinal Plant

    Directory of Open Access Journals (Sweden)

    Nahid Babaei

    2014-01-01

    Full Text Available A procedure was developed for in vitro propagation of Curculigo latifolia through shoot tip culture. Direct regeneration and indirect scalp induction of Curculigo latifolia were obtained from shoot tip grown on MS medium supplemented with different concentrations and combinations of thidiazuron and indole-3-butyric acid. Maximum response for direct regeneration in terms of percentage of explants producing shoot, shoot number, and shoot length was obtained on MS medium supplemented with combination of thidiazuron (0.5 mg L−1 and indole-3-butyric acid (0.25 mg L−1 after both 10 and 14 weeks of cultures. Indole-3-butyric acid in combination with thidiazuron exhibited a synergistic effect on shoot regeneration. The shoot tips were able to induce maximum scalp from basal end of explants on the medium with 2 mg L−1 thidiazuron. Cultures showed that shoot number, shoot length, and scalp size increased significantly after 14 weeks of culture. Transferring of the shoots onto the MS medium devoid of growth regulators resulted in the highest percentage of root induction and longer roots, while medium supplemented with 0.25 mg L−1 IBA produced more numbers of roots.

  7. Transgenic Rice Plants Harboring Genomic DNA from Zizania latifolia Confer Bacterial Blight Resistance

    Institute of Scientific and Technical Information of China (English)

    SHEN Wei-wei; SONG Cheng-li; CHEN Jie; Fu Ya-ping; Wu Jian-li; JIANG Shao-mei

    2011-01-01

    Based on the sequence of a resistance gene analog FZ14 derived from Zizania latifolia (Griseb.),a pair of specific PCR primers FZ14P1/FZ14P2 was designed to isolate candidate disease resistance gene.The pooled-PCR approach was adopted using the primer pair to screen a genomic transformation-competent artificial chromosome (TAC) library derived from Z.latifolia.A positive TAC clone (ZR1) was obtained and confirmed by sequence analysis.The results indicated that ZR1 consisted of conserved motifs similar to P-loop (kinase 1a),kinase 2,kinase 3a and GLPL (Gly-Leu-Pro-Leu),suggesting that it could be a portion of NBS-LRR type of resistance gene.Using Agrobacterium-mediated transformation of Nipponbare mature embryo,a total of 48 independent transgenic T0 plants were obtained.Among them,36 plants were highly resistant to the virulent bacterial blight strain P×O71.The results indicate that ZR1 contains at least one functional bacterial blight resistance gene.

  8. Well-being perceived and working conditions at ENEA research center; La valutazione soggettiva del benessere lavorativo in un campione di dipendenti ENEA dell'area bolognese

    Energy Technology Data Exchange (ETDEWEB)

    Cenni, P.; Fagarazzi, G. [ENEA, Centro Ricerche Ezio Clementel, Bologna (Italy). Dipt. Ambiente; Sarchielli, M.; Zanobini, F. [Bologna Univ., Bologna (Italy). Dipt. di Psicologia

    1999-07-01

    The aim of this research on 143 workers of the ENEA area in Bologna, has been to survey the degree of well-being perceived and expressed by these workers with respect to different factors of the work environment. This study follows some training/information seminars on environment, health and safety organized in ENEA by Occupational Medicine Group to comply with Leg. Decr. 626/94. In a multidisciplinary approach the health idea has been interpreted not as lack of illness but as pursuit and preservation of the well-being in the work environment. For this purpose, also the involvement and participation of the workers seem to be very useful for subjective reports about individual task, equipment, interfaces, workplace and work organization. In this research, we have adopted an ergonomic checklist based on guiding principles to be applied to the design of optimal working conditions with regard to human well-being, safety and health (see UNI ENV 26385, 1991). Data processing and analysis have requested occupational medicine, ergonomics and statistics competencies. [Italian] La ricerca condotta su un campione ENEA di 143 dipendenti dell'area bolognese ha inteso verificare il grado di benessere percepito ed espresso dai lavoratori in rapporto alle diverse variabili presenti nel contesto lavorativo. Tale indagine e' stata preceduta da specifici seminari di formazione/informazione su ambiente, salute e sicurezza, organizzati a cura della Medicina del Lavoro di Bologna presso le sedi ENEA, a seguito delle disposizioni contenute nel D.Lvo. 626/94. In un'ottica multidisciplinare, il concetto di salute e' stato interpretato non tanto come assenza di malattia quanto come ricerca e mantenimento del benessere lavorativo e, per raggiungere questo scopo, sembra essere molto utile anche il diretto coinvolgimento e la partecipazione dei lavoratori per valutazioni soggettive sulle mansioni assegnate, le attrezzature, le interfacce, la postazione di lavoro e l

  9. Ethanol fermentation of mahula (Madhuca latifolia L.) flowers using free and immobilized yeast Saccharomyces cerevisiae.

    Science.gov (United States)

    Swain, M R; Kar, S; Sahoo, A K; Ray, R C

    2007-01-01

    There is a growing interest to find alternate bioresources for production of ethanol, apart from cane/sugar beet molasses and starchy crops like sweet sorghum, cassava and sweet potato. Mahula (Madhuca latifolia L.) is a forest tree abundantly available in the Indian subcontinent and its flowers are very rich in fermentable sugars (28.1-36.3 g 100 g(-1)). Batch fermentation of fresh and 12-month-stored flowers with free (whole cells) and immobilized cells of Saccharomyces cerevisiae (strain CTCRI) was carried out in 2-l Erlenmeyer flasks. The ethanol yields were 193 and 148 g kg(-1) (using free cells) and 205 and 152 g kg(-1) (using immobilized cells) from fresh and 12-month-stored mahula flowers, respectively. PMID:16580830

  10. Bioethanol production from mahula (Madhuca latifolia L.) flowers by solid-state fermentation

    Energy Technology Data Exchange (ETDEWEB)

    Mohanty, Sujit Kumar [Division of Biotechnology, Majhighariani Institute of Technology and Science, Sriram Vihar, Bhujabal, Rayagada 765017 (India); Behera, Shuvasis [Department of Botany, Utkal University, Bhubaneswar 751004 (India); Swain, Manas Ranjan; Ray, Ramesh Chandra [Central Tuber Crops Research Institute (Regional Centre), Bhubaneswar 751019 (India)

    2009-05-15

    There is a growing interest worldwide to find out new and cheap carbohydrate sources for production of bioethanol. In this context, the production of ethanol from mahula (Madhuca latifolia L.) flowers by Saccharomyces cerevisiae in solid-state fermentation was investigated. The moisture level of 70%, pH of 6.0 and temperature of 30 C were found optimum for maximum ethanol concentration (225.0 {+-} 4.0 g/kg flower) obtained from mahula flowers after 72 h of fermentation. Concomitant with highest ethanol concentration, the maximum ethanol productivity (3.13 g/kg flower/h), yeast biomass (18.5 x 10{sup 8} CFU/g flower), the ethanol yield (58.44 g/100 g sugar consumed) and the fermentation efficiency (77.1%) were also obtained at these parametric levels. (author)

  11. Characterization of the bacterial flora associated with root systems of Pinus contorta var. latifolia.

    Science.gov (United States)

    Dangerfield, J A; Westlake, D W; Cook, F D

    1978-12-01

    Root systems of young and mature lodgepole pine (Pinus contorta Dougl. var. latifolia Englem.) were removed from forest stands and the associated aerobic bacterial flora isolated. Characterization of rhizoplane and control soil isolates from these tree root systems demonstrated differences from that reported for agricultural crops. Ammonifying, proteolytic, and amylolytic organisms were proportionately reduced within the rhizoplane. The rhizoplane organisms grew more slowly than the control soil isolates, although they responded in greater numbers to the addition of an amino acid supplement to the growth media. The rhizoplane organisms also showed an increased ability to solubilize phosphate. The chitinolytic organisms were suppressed within the rhizoplane of the mature tree but were stimulated by the young trees. With this exception, the rhizoplane microflora of older and younger trees were similar. PMID:747813

  12. Detection of Heavy Metal Contents and Proximate Analysis of roots of Anogeissus latifolia

    Directory of Open Access Journals (Sweden)

    Kishor K. Dholwani

    2013-03-01

    Full Text Available The study of heavy metal contamination in plants, serve to indicate the metal concentration status of the site, where plants have grown. This is an important tool for phytoremediation. Proximate analysis in plants gives valuable information and help to access the quality of the sample. So studies were carried out on the roots of Anogeissus latifolia which is useful in UTI infections, skin diseases, liver complaints, fever, epileptic fits etc. for heavy metal contents and proximate analysis of the root sample was also done. The root showed the absence of heavy metals when it was subjected to analysis by Inductively Coupled Plasma Atomic Spectrometer. The ash values, extractive values, moisture content, total solid content and crude fiber content of the root sample were also determined.

  13. Anatomy and Histochemistry of Roots and Shoots in Wild Rice (Zizania latifolia Griseb.

    Directory of Open Access Journals (Sweden)

    Chaodong Yang

    2014-01-01

    Full Text Available Wild rice (Zizania latifolia Griseb. is a famous, perennial, emergent vegetable in China. The current work explores the anatomy and histochemistry of roots, stems, and leaves and the permeability of apoplastic barriers of wild rice. The adventitious roots in wild rice have suberized and lignified endodermis and adjacent, thick-walled cortical layers and suberized and lignified hypodermis, composed of a uniseriate sclerenchyma layer (SC underlying uniseriate exodermis; they also have lysigenous aerenchyma. Stems have a thickened epidermal cuticle, a narrow peripheral mechanical ring (PMR, an outer ring of vascular bundles, and an inner ring of vascular bundles embedded in a multiseriate sclerenchyma ring (SCR. There is evidence of suberin in stem SCR and PMR sclerenchyma cells. Sheathing leaves are characterized by thick cuticles and fibrous bundle sheath extensions. Air spaces in stems and leaves consist of mostly lysigenous aerenchyma and pith cavities in stems. Apoplastic barriers are found in roots and stems.

  14. Bioflocculant exopolysaccharide production by Azotobacter indicus using flower extract of Madhuca latifolia L.

    Science.gov (United States)

    Patil, Satish V; Salunkhe, Rahul B; Patil, Chandrashekhar D; Patil, Deepak M; Salunke, Bipinchandra K

    2010-10-01

    Efficacy of Azotobacter indicus ATCC 9540 strain for production exopolysaccharide (EPS) bioflocculant was investigated. Mahua flower extract (Madhuca latifolia L), a natural substrate at the concentration of 20 g L(-1), gave maximum recovery of EPS followed by sucrose and mannitol as compared to other carbon sources after 172 h. Yeast extract was found to be the most effective nitrogen source as compared to beef extract, sodium nitrate, ammonium sulfate, casein hydrolysate, and urea for the production of EPS. EPS production was increased in presence of nitrogen (5.51 g L(-1)) as compared to nitrogen-free medium (3.51 g L(-1)), and fermentation time was also reduced by 28 h. Maximum EPS production (6.10 g L(-1)) was found in the presence of 20 g L(-1) flower extract and 0.5 g L(-1) yeast extract containing Ashby's media with 180 rpm at 30 degrees C at 144 h, under controlled conditions in 2.5 L fermenter using optimized medium. The isolated EPS showed cation-dependent flocculating activity. Concentration of EPS played an important role in bioflocculating activity which increased in a concentration-dependent manner up to a certain limit, with the maximum flocculation of 72% at 500 mg L(-1) concentration but remained almost static after this concentration. Extracted polymer was characterized by different chemical tests, FT-IR spectroscopy, and TLC which showed presence of uronic acids, O-acetyl groups, and Orcinol with suggestive indication of alginate like polymer. This study suggests that use of M. latifolia L. flowers can be a potential alternative bioresource for production of exopolysaccharide. PMID:19921493

  15. Noble strain of Sparassis latifolia produces high content of β-glucan

    Institute of Scientific and Technical Information of China (English)

    Dong; Ju; Lee; Min; Cheol; Jang; A; Ra; Jo; Hyun; Jun; Choi; Kwang-Sang; Kim; Youn-Tae; Chi

    2015-01-01

    Objective: To classify four new Sparassis strains(CLM1, CKM1, CKM2, and KJM1) using the internal transcribed spacer sequence and to elucidate their β-glucan content and mycelial growth.Methods: Two different microbiological media were used to determine growth rate. The β-glucan contents were analyzed using the Megazyme Mushroom and Yeast Beta-Glucan kit. To determine the genetic relationships, phylogenetic trees were constructed using ClustalX. Multiple sequence alignments were printed and shaded with the BOXSHADE 3.21 program. Results: In this study, four new Sparassis strains were isolated from the southern region of the Korea Peninsula. They were all classified into the Sparassis latifolia clade as a monophyletic group based on the internal transcribed spacer sequence. Mycelial growth rate of the CLM1 strain was highest in potato dextrose agar and potato dextrose agar larch. The β-glucan content of the CLM1 strain was highest at 29.5%(w/w). A high degree of sequence divergence was detected in the RNA polymerase second largest subunit II gene(RPB2) within Sparassis spp. tested. The putative amino acid sequences of the RPB2 had a distinct sequence. The nucleotide sequences of the RPB2’s intron were also divergent among Sparassis spp., even though their nucleotide length was well conserved within Sparassis latifolia. Conclusions: These results indicate that the nucleotide sequences and the amino acid sequences of RPB2 can be used to identify individual Sparassis sp. The Sparassis strain CLM1 may be best for developing a remedy to prevent or treat cancer and other chronic diseases.

  16. Biological control of golden apple snail, Pomacea canaliculata by Chinese soft-shelled turtle, Pelodiscus sinensis in the wild rice, Zizania latifolia field

    OpenAIRE

    Shengzhang Dong; Guowan Zheng; Xiaoping Yu; Changhuan Fu

    2012-01-01

    The wild rice, Zizania latifolia Turcz, used to be one of the important aquatic vegetables cultivated in China. Recently, the golden apple snail - GAS (Pomacea canaliculata (Lamarck)) was found to be a major invasive pest attacking Z. latifolia. To control efficiently GAS, predation by the Chinese soft-shelled turtles (Pelodiscus sinensis) on GAS was evaluated in laboratory and field trials. P. sinensis had a strong predatory capacity and selectivity for GAS both in laboratory and field condi...

  17. 蒲菜罐头加工工艺的优化%Optimization of Processing Technic of Typha latifolia Can

    Institute of Scientific and Technical Information of China (English)

    万国福

    2012-01-01

    In order to enrich the food types and methods of T. latifolia and eliminate rush food seasonal limited, and provide vast thinking for deep processing the characteristic agricultural product T. latifolia. Taking T. latifolia as the major raw material, the processing technic was optimized and unique T. latifolia can was produced. According to analyzing the ratio of material to water, the time of color-protection, the concentration of color-protection, salt, monosodium glutamate and chilies, the optimum processing conditions were got as follows: T. latifolia-water ratio (g : mL) 1 :1, color-protection time 1 minute, 0. 15% Na2SO3 , 0. 2% salt, 1. 2% monosodium glutamate, 1. 0% dried chilli and 0. 2% five spices. Under these conditions, T. latifolia can could be obtained with milky color, crisp taste, aromatic flavor and rich nutrient.%为了丰富蒲菜的食用种类和方法,打破蒲菜季节性时令性局限,为淮安特色农产品蒲菜的精深加工提供广阔的思路,以蒲菜为主要原料,对蒲菜罐头加工工艺进行优化,制得风味独特的蒲菜罐头.试验通过对料水比、护色时间、护色剂浓度、食盐、味精、五香粉等因素的试验分析,得到特色风味蒲菜罐头制作的最佳工艺条件,即料水比(g:mL)为1:1、护色时间1 min、护色剂亚硫酸钠浓度0.15%、食盐0.2%、味精1.2%、干辣椒1.0%、五香粉0.2%,在此条件下能得到色泽乳白、口感清脆、香味浓郁、营养丰富的蒲菜罐头.

  18. Observações sobre o modo de reprodução em Adesmia latifolia Spreng. Vog. Observations on the mode of reproduction of Adesmia latifolia Spreng. Vog.

    Directory of Open Access Journals (Sweden)

    Solange Bosio Tedesco

    1998-03-01

    Full Text Available Um experimento foi conduzido em casa de vegetação, com plantas de um acesso de Adesmia latifolia, para fazer observações relativas ao seu modo de reprodução. As plantas foram isoladas individualmente e, após o florescimento, foram submetidas a três tratamentos: estímulo mecânico, cruzamentos manuais e isolamento. Os resultados indicaram versatilidade da espécie, a qual pode reproduzir-se por fecundação cruzada e por autofecundação.An experiment was carried out to study the reproduction mode of Adesmia latifolia. The experiment was conducted in greenhouse with plants of one access of this species. The plants after flowering were isolated individually and submitted to three treatments: mechanical stimulation, hand crossing and isolation. The results showed versatility of this species which could reproduce by cross-fertilization and self-fertilization.

  19. Evaluation of Anti-Inflammatory Activity of Citrus latifolia Tanaka Essential Oil and Limonene in Experimental Mouse Models

    OpenAIRE

    Raquel Kummer; Fernanda Carolina Fachini-Queiroz; Camila Fernanda Estevão-Silva; Renata Grespan; Expedito Leite Silva; Ciomar Aparecida Bersani-Amado; Roberto Kenji Nakamura Cuman

    2013-01-01

    The genus Citrus (Rutaceae) includes several species of plants that produce some of the most cultivated fruits in the world, providing an appreciable content of essential oil. In folk medicine, they are used as a cholagogue, antipyretic, anti-inflammatory, sedative, and antitoxic effects. Lemon essential oil has been used since ancient times for its antiseptic, carminative, diuretic, and eupeptic effects. In this study, we investigated the anti-inflammatory activity of Citrus latifolia Tanaka...

  20. Study on Color Protection Technology of Typha latifolia L.%蒲菜护色技术研究

    Institute of Scientific and Technical Information of China (English)

    董静; 魏福华

    2013-01-01

      以新鲜的蒲菜为原料,研究烫漂条件、柠檬酸、氯化钠、抗坏血酸等护色剂对蒲菜色泽的影响.结果表明,蒲菜经过100℃/3 min漂烫处理后,用复合护色液浸泡30 min,能较好地保持蒲菜原有的色泽和质地,蒲菜最佳护色液组合为0.1%CA、0.5%NaCl、0.09%VC、1.5%CaCl.%Using the Typha latifolia L. as raw materials, the effects of blanching conditions and different color fixatives (citric acid, sodium chloride, Vitamin C) on color of Typha latifolia L. were studied. The results showed that the original color and texture of Typha latifolia L. can be well retained with blanching at 100℃for 3 min, then soaking in compound color protection solution with 30 min.The optimal compound color protection solution was:0.1%citricacid, 0.5%sodium chloride,0.09%VitaminC,1.5%calciumchloride.

  1. Seed germination characteristics of Phillyrea angustifolia L. and P. latifolia L. (Oleaceae, two Mediterranean shrub species having lignified endocarp

    Directory of Open Access Journals (Sweden)

    Sara Mira

    2015-04-01

    Full Text Available The aim of this study was to determine the germination characteristics of Phillyrea angustifolia L. and P. latifolia L. seeds in order to develop an optimized propagation protocol for Phillyrea species. Seeds of P. angustifolia and P. latifolia were collected from wild plants growing in Cáceres province (CW Spain and Andalucía (S Spain, respectively. Percentage of water uptake for P. latifolia seeds was calculated. Seeds with and without endocarp were germinated at different constant and alternating temperatures. Seeds without endocarp were soaked in distilled water or gibberellic acid, and then set to germinate. Seeds with endocarp of both species were stratified at 5 ºC for 30 or 90 days and then the endocarp was completely removed from the seeds before they were sowed. Chemical scarification with sulfuric acid and mechanical scarification were tested on P. angustifolia seeds with endocarp. Phillyrea endocarp was permeable to water, since Phillyrea seeds with endocarp imbibed water, but water uptake was faster when the endocarp was removed. Moreover, the encodarp could interfere mechanically in the emergence of the radicle, since seed germination of Phillyrea species was promoted by the complete removal of the lignified endocarp surrounding each seed. Optimal germination temperature for both species was 15 ºC, and lower temperatures produced secondary dormancy. Soaking in distilled water or gibberellic acid did not significantly enhance seed germination. Cold stratification and chemical scarification treatments were detrimental for seed germination.

  2. Neutron Activation Foil and Thermoluminescent Dosimeter Responses to a Polyethylene Reflected Pulse of the CEA Valduc SILENE Critical Assembly

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Thomas Martin [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Celik, Cihangir [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); McMahan, Kimberly L. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lee, Yi-kang [French Atomic Energy Commission (CEA), Saclay (France); Gagnier, Emmanuel [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette; Authier, Nicolas [French Atomic Energy Commission (CEA), Salives (France). Valduc Centre for Nuclear Studies; Piot, Jerome [French Atomic Energy Commission (CEA), Salives (France). Valduc Centre for Nuclear Studies; Jacquet, Xavier [French Atomic Energy Commission (CEA), Salives (France). Valduc Centre for Nuclear Studies; Rousseau, Guillaume [French Atomic Energy Commission (CEA), Salives (France). Valduc Centre for Nuclear Studies; Reynolds, Kevin H. [Y-12 National Security Complex, Oak Ridge, TN (United States)

    2016-09-01

    This benchmark experiment was conducted as a joint venture between the US Department of Energy (DOE) and the French Commissariat à l'Energie Atomique (CEA). Staff at the Oak Ridge National Laboratory (ORNL) in the US and the Centre de Valduc in France planned this experiment. The experiment was conducted on October 19, 2010 in the SILENE critical assembly facility at Valduc. Several other organizations contributed to this experiment and the subsequent evaluation, including CEA Saclay, Lawrence Livermore National Laboratory (LLNL), the Y-12 National Security Complex (NSC), Babcock International Group in the United Kingdom, and Los Alamos National Laboratory (LANL). The goal of this experiment was to measure neutron activation and thermoluminescent dosimeter (TLD) doses from a source similar to a fissile solution critical excursion. The resulting benchmark can be used for validation of computer codes and nuclear data libraries as required when performing analysis of criticality accident alarm systems (CAASs). A secondary goal of this experiment was to qualitatively test performance of two CAAS detectors similar to those currently and formerly in use in some US DOE facilities. The detectors tested were the CIDAS MkX and the Rocky Flats NCD-91. The CIDAS detects gammas with a Geiger-Muller tube and the Rocky Flats detects neutrons via charged particles produced in a thin 6LiF disc depositing energy in a Si solid state detector. These detectors were being evaluated to determine whether they would alarm, so they were not expected to generate benchmark quality data.

  3. Neutron Activation Foil and Thermoluminescent Dosimeter Responses to a Lead Reflected Pulse of the CEA Valduc SILENE Critical Assembly

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Thomas Martin [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Celik, Cihangir [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Isbell, Kimberly McMahan [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lee, Yi-kang [Commissariat a l' Energie Atomique et aux Energies Alternatives (CEA-Saclay), Gif-sur-Yvette (France); Gagnier, Emmanuel [Commissariat a l' Energie Atomique et aux Energies Alternatives (CEA-Saclay), Gif-sur-Yvette (France); Authier, Nicolas [Commissariat a l' Energie Atomique et aux Energies Alternatives (CEA-Saclay), Gif-sur-Yvette (France); Piot, Jerome [Commissariat a l' Energie Atomique et aux Energies Alternatives (CEA-Saclay), Gif-sur-Yvette (France); Jacquet, Xavier [Commissariat a l' Energie Atomique et aux Energies Alternatives (CEA-Saclay), Gif-sur-Yvette (France); Rousseau, Guillaume [Commissariat a l' Energie Atomique et aux Energies Alternatives (CEA-Saclay), Gif-sur-Yvette (France); Reynolds, Kevin H. [Y-12 National Security Complex, Oak Ridge, TN (United States)

    2016-09-01

    This benchmark experiment was conducted as a joint venture between the US Department of Energy (DOE) and the French Commissariat à l'Energie Atomique (CEA). Staff at the Oak Ridge National Laboratory (ORNL) in the US and the Centre de Valduc in France planned this experiment. The experiment was conducted on October 13, 2010 in the SILENE critical assembly facility at Valduc. Several other organizations contributed to this experiment and the subsequent evaluation, including CEA Saclay, Lawrence Livermore National Laboratory (LLNL), the Y-12 National Security Complex (NSC), Babcock International Group in the United Kingdom, and Los Alamos National Laboratory (LANL). The goal of this experiment was to measure neutron activation and thermoluminescent dosimeter (TLD) doses from a source similar to a fissile solution critical excursion. The resulting benchmark can be used for validation of computer codes and nuclear data libraries as required when performing analysis of criticality accident alarm systems (CAASs). A secondary goal of this experiment was to qualitatively test performance of two CAAS detectors similar to those currently and formerly in use in some US DOE facilities. The detectors tested were the CIDAS MkX and the Rocky Flats NCD-91. The CIDAS detects gammas with a Geiger-Muller tube, and the Rocky Flats detects neutrons via charged particles produced in a thin 6LiF disc, depositing energy in a Si solid-state detector. These detectors were being evaluated to determine whether they would alarm, so they were not expected to generate benchmark quality data.

  4. Neutron Activation and Thermoluminescent Detector Responses to a Bare Pulse of the CEA Valduc SILENE Critical Assembly

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Thomas Martin [ORNL; Isbell, Kimberly McMahan [ORNL; Lee, Yi-kang [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette; Gagnier, Emmanuel [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette; Authier, Nicolas [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille; Piot, Jerome [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille; Jacquet, Xavier [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille; Rousseau, Guillaume [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille; Reynolds, Kevin H. [Y-12 National Security Complex

    2016-09-01

    This benchmark experiment was conducted as a joint venture between the US Department of Energy (DOE) and the French Commissariat à l'Energie Atomique (CEA). Staff at the Oak Ridge National Laboratory (ORNL) in the US and the Centre de Valduc in France planned this experiment. The experiment was conducted on October 11, 2010 in the SILENE critical assembly facility at Valduc. Several other organizations contributed to this experiment and the subsequent evaluation, including CEA Saclay, Lawrence Livermore National Laboratory (LLNL), the Y-12 National Security Complex (NSC), Babcock International Group in the United Kingdom, and Los Alamos National Laboratory (LANL). The goal of this experiment was to measure neutron activation and thermoluminescent dosimeter (TLD) doses from a source similar to a fissile solution critical excursion. The resulting benchmark can be used for validation of computer codes and nuclear data libraries as required when performing analysis of criticality accident alarm systems (CAASs). A secondary goal of this experiment was to qualitatively test performance of two CAAS detectors similar to those currently and formerly in use in some US DOE facilities. The detectors tested were the CIDAS MkX and the Rocky Flats NCD-91. These detectors were being evaluated to determine whether they would alarm, so they were not expected to generate benchmark quality data.

  5. Transcription profiles of mitochondrial genes correlate with mitochondrial DNA haplotypes in a natural population of Silene vulgaris

    Directory of Open Access Journals (Sweden)

    Olson Matthew S

    2010-01-01

    Full Text Available Abstract Background Although rapid changes in copy number and gene order are common within plant mitochondrial genomes, associated patterns of gene transcription are underinvestigated. Previous studies have shown that the gynodioecious plant species Silene vulgaris exhibits high mitochondrial diversity and occasional paternal inheritance of mitochondrial markers. Here we address whether variation in DNA molecular markers is correlated with variation in transcription of mitochondrial genes in S. vulgaris collected from natural populations. Results We analyzed RFLP variation in two mitochondrial genes, cox1 and atp1, in offspring of ten plants from a natural population of S. vulgaris in Central Europe. We also investigated transcription profiles of the atp1 and cox1 genes. Most DNA haplotypes and transcription profiles were maternally inherited; for these, transcription profiles were associated with specific mitochondrial DNA haplotypes. One individual exhibited a pattern consistent with paternal inheritance of mitochondrial DNA; this individual exhibited a transcription profile suggestive of paternal but inconsistent with maternal inheritance. We found no associations between gender and transcript profiles. Conclusions Specific transcription profiles of mitochondrial genes were associated with specific mitochondrial DNA haplotypes in a natural population of a gynodioecious species S. vulgaris. Our findings suggest the potential for a causal association between rearrangements in the plant mt genome and transcription product variation.

  6. Neutron Activation and Thermoluminescent Detector Responses to a Bare Pulse of the CEA Valduc SILENE Critical Assembly

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Thomas Martin [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Celik, Cihangir [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); McMahan, Kimberly L. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lee, Yi-kang [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette (France); Gagnier, Emmanuel [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette (France); Authier, Nicolas [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Piot, Jerome [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Jacquet, Xavier [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Rousseau, Guillaume [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Reynolds, Kevin H. [Oak Ridge Y-12 Plant (Y-12), Oak Ridge, TN (United States)

    2015-09-01

    This benchmark experiment was conducted as a joint venture between the US Department of Energy (DOE) and the French Commissariat à l'Energie Atomique (CEA). Staff at the Oak Ridge National Laboratory (ORNL) in the US and the Centre de Valduc in France planned this experiment. The experiment was conducted on October 11, 2010 in the SILENE critical assembly facility at Valduc. Several other organizations contributed to this experiment and the subsequent evaluation, including CEA Saclay, Lawrence Livermore National Laboratory (LLNL), the Y-12 National Security Complex (NSC), Babcock International Group in the United Kingdom, and Los Alamos National Laboratory (LANL). The goal of this experiment was to measure neutron activation and thermoluminescent dosimeter (TLD) doses from a source similar to a fissile solution critical excursion. The resulting benchmark can be used for validation of computer codes and nuclear data libraries as required when performing analysis of criticality accident alarm systems (CAASs). A secondary goal of this experiment was to qualitatively test performance of two CAAS detectors similar to those currently and formerly in use in some US DOE facilities. The detectors tested were the CIDAS MkX and the Rocky Flats NCD-91. These detectors were being evaluated to determine whether they would alarm, so they were not expected to generate benchmark quality data.

  7. Evaluation of the concrete shield compositions from the 2010 criticality accident alarm system benchmark experiments at the CEA Valduc SILENE facility

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Thomas Martin [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Celik, Cihangir [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Dunn, Michael E [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Wagner, John C [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); McMahan, Kimberly L [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Authier, Nicolas [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Jacquet, Xavier [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Rousseau, Guillaume [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Wolff, Herve [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Savanier, Laurence [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Baclet, Nathalie [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Lee, Yi-kang [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette (France); Trama, Jean-Christophe [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette (France); Masse, Veronique [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette (France); Gagnier, Emmanuel [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette (France); Naury, Sylvie [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette (France); Blanc-Tranchant, Patrick [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette (France); Hunter, Richard [Babcock International Group (United Kingdom); Kim, Soon [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Dulik, George Michael [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Reynolds, Kevin H. [Y-12 National Security Complex, Oak Ridge, TN (United States)

    2015-01-01

    In October 2010, a series of benchmark experiments were conducted at the French Commissariat a l'Energie Atomique et aux Energies Alternatives (CEA) Valduc SILENE facility. These experiments were a joint effort between the United States Department of Energy Nuclear Criticality Safety Program and the CEA. The purpose of these experiments was to create three benchmarks for the verification and validation of radiation transport codes and evaluated nuclear data used in the analysis of criticality accident alarm systems. This series of experiments consisted of three single-pulsed experiments with the SILENE reactor. For the first experiment, the reactor was bare (unshielded), whereas in the second and third experiments, it was shielded by lead and polyethylene, respectively. The polyethylene shield of the third experiment had a cadmium liner on its internal and external surfaces, which vertically was located near the fuel region of SILENE. During each experiment, several neutron activation foils and thermoluminescent dosimeters (TLDs) were placed around the reactor. Nearly half of the foils and TLDs had additional high-density magnetite concrete, high-density barite concrete, standard concrete, and/or BoroBond shields. CEA Saclay provided all the concrete, and the US Y-12 National Security Complex provided the BoroBond. Measurement data from the experiments were published at the 2011 International Conference on Nuclear Criticality (ICNC 2011) and the 2013 Nuclear Criticality Safety Division (NCSD 2013) topical meeting. Preliminary computational results for the first experiment were presented in the ICNC 2011 paper, which showed poor agreement between the computational results and the measured values of the foils shielded by concrete. Recently the hydrogen content, boron content, and density of these concrete shields were further investigated within the constraints of the previously available data. New computational results for the first experiment are now available

  8. Nutrients removal by Typha latifolia and Cynodon spp. grown in constructed wetlands

    Directory of Open Access Journals (Sweden)

    Mateus Pimentel de Matos

    2011-04-01

    Full Text Available The objective of this study was to evaluate the extraction capacity of two species when grown in constructed wetlands with subsurface horizontal flow (SACs for the treatment of swine wastewater (ARS. To this end, were built 8 SACs of 2.0 m x 0.5 m x 0.6 m, fiber glass, filled with 0.55 m of fine gravels. In SAC2; SAC4; SAC6 and SAC8 was cultivated cattail (Typha latifolia and in SAC3; SAC5; SAC7 and SAC9 was cultivated tifton-85 bermudagrass (Cynodon spp.. The SAC2 and SAC3, SAC4 and SAC5, SAC6 and SAC7 and SAC8 and SAC9 received 163, 327, 461 and 561 kg ha-1 day-1 of BOD, respectively. During the 120 days of the SACs monitoring, it was found that the cattail has not adapted to the conditions of exposure. The highest yields were obtained with the application of organic load average of 327 kg ha-1 day-1 of BOD. The tifton-85 was the plant species with the highest capacity to extract nutrients, getting to draw between 443 and 540, 86 and 99, 193 and 241, 0.77 and 2.17, and 1.21 and 3.68 kg ha-1 TKN, P, K, Cu and Zn, respectively, while cattail showed greater capacity to absorb sodium.

  9. Effect of phenol on the agronomic performance of Typha latifolia L.

    Directory of Open Access Journals (Sweden)

    Antonio Teixeira de Matos

    2009-12-01

    Full Text Available This study was carried out to evaluate the effect from phenol application in macrophytes (Typha latifolia L., to use in constructed wetlands used to wastewater treatment. Typha was cropped in 15 L pots under greenhouse conditions. The pots were infilled with crushed stone (zero and nutritious solution of Hoagland modified. The effects were evaluated for the following application doses: 0 (T0, 150 (T1, 300 (T2, 450 (T3 e 600 mg L-1 of phenol (T4. Control pots no planting received 300 mg L-1 of phenol (T5. Weekly, it was made replacement of water evapotranspired (T1, T2, T3 and T4 or evaporated (T5. Phenol concentration increased in nutritious solution in pots of T3, T4, T5 and tendency of stabilization in pots T1 and T2 was verified. The highest yield of dry matter occurred in that treatment T0 and T1. Typha presents increase in take up nitrogen and phosphorus in pots submitted to the solution and larger phenol concentration. However, the smallest biomass production led to a smaller extraction of nutrients in nutritious solution. The results indicated that Typha was able to develop satisfactorily in nutrient solution with concentration of phenol below 150 mg L-1.

  10. The alpine cushion plant Silene acaulis as foundation species: a bug's-eye view to facilitation and microclimate.

    Directory of Open Access Journals (Sweden)

    Olivia Molenda

    Full Text Available Alpine ecosystems are important globally with high levels of endemic and rare species. Given that they will be highly impacted by climate change, understanding biotic factors that maintain diversity is critical. Silene acaulis is a common alpine nurse plant shown to positively influence the diversity and abundance of organisms--predominantly other plant species. The hypothesis that cushion or nurse plants in general are important to multiple trophic levels has been proposed but rarely tested. Alpine arthropod diversity is also largely understudied worldwide, and the plant-arthropod interactions reported are mostly negative, that is,. herbivory. Plant and arthropod diversity and abundance were sampled on S. acaulis and at paired adjacent microsites with other non-cushion forming vegetation present on Whistler Mountain, B.C., Canada to examine the relative trophic effects of cushion plants. Plant species richness and abundance but not Simpson's diversity index was higher on cushion microsites relative to other vegetation. Arthropod richness, abundance, and diversity were all higher on cushion microsites relative to other vegetated sites. On a microclimatic scale, S. acaulis ameliorated stressful conditions for plants and invertebrates living inside it, but the highest levels of arthropod diversity were observed on cushions with tall plant growth. Hence, alpine cushion plants can be foundation species not only for other plant species but other trophic levels, and these impacts are expressed through both direct and indirect effects associated with altered environmental conditions and localized productivity. Whilst this case study tests a limited subset of the membership of alpine animal communities, it clearly demonstrates that cushion-forming plant species are an important consideration in understanding resilience to global changes for many organisms in addition to other plants.

  11. Phylogeographic pattern of range expansion provides evidence for cryptic species lineages in Silene nutans in Western Europe.

    Science.gov (United States)

    Martin, H; Touzet, P; Van Rossum, F; Delalande, D; Arnaud, J-F

    2016-03-01

    As a result of recent or past evolutionary processes, a single species might consist of distinct Evolutionary Significant Units (ESUs), even corresponding to cryptic species. Determining the underlying mechanisms of range shifts and the processes at work in the build-up of divergent ESUs requires elucidating the factors that contribute to population genetic divergence across a species' range. We investigated the large-scale patterns of genetic structure in the perennial herbaceous plant species Silene nutans (Caryophyllaceae) in Western Europe. We sampled and genotyped 111 populations using 13 nuclear microsatellite loci and 6 plastid single-nucleotide polymorphisms. Broad-scale spatial population genetic structure was examined using Bayesian clustering, spatial multivariate analyses and measures of hierarchical genetic differentiation. The genotypic structure of S. nutans was typical of a predominantly allogamous mating system. We also identified plastid lineages with no intra-population polymorphism, mirroring two genetically differentiated nuclear lineages. No evidence of admixture was found. Spatial trends in genetic diversity further suggested independent leading-edge expansion associated with founding events and subsequent genetic erosion. Overall, our findings suggested speciation processes in S. nutans and highlighted striking patterns of distinct stepwise recolonisation of Western Europe shaped by Quaternary climate oscillations. Two main potential ESUs can be defined in Western Europe, corresponding to Eastern and Western nuclear-plastid lineages. In situ preservation of populations and genetic rescue implying ex situ conservation techniques should take the lineage identity into account. This is particularly true in Great Britain, northern France and Belgium, where S. nutans is rare and where distinct lineages co-occur in close contact. PMID:26647652

  12. High-level Zn and Cd tolerance in Silene paradoxa L. from a moderately Cd- and Zn-contaminated copper mine tailing

    International Nuclear Information System (INIS)

    Cadmium and zinc tolerance were examined in populations of Silene paradoxa, one from uncontaminated calcareous soil (CVD) and one from a mine tailing (FC) (Cd < 1-15 ppm, Zn 400-1300 ppm, pH 2-6). The mine population exhibited extremely high Zn and Cd tolerance levels, although the degrees of Cd and Zn enrichment relatively low at the population site. Cd and Zn hypertolerance in FC were associated with reduced rates of accumulation of these metals, both in roots and shoots (Cd), or exclusively in shoots (Zn). However, exclusion potentially explained only a minor part of the superior tolerance in FC. Cd hypertolerance in FC was associated with decreased, rather than enhanced phytochelatin accumulation. The remarkably high levels of Cd and Zn hypertolerance in FC might relate to the low soil pH, due to oxidation of sulphide minerals, and the absence of soil organic matter at the FC site. - Silene paradoxa from a copper mine exhibits extreme levels of Zn and Cd tolerance

  13. Biomass production of young lodgepole pine (Pinus contorta var. latifolia stands in Latvia

    Directory of Open Access Journals (Sweden)

    Jansons A

    2013-01-01

    Full Text Available Biomass as a source of renewable energy is gaining an increasing importance in the context of emission targets set by the European Union. Large areas of abandoned agricultural land with different soils are potentially available for establishment of biomass plantations in the Baltic states. Considering soil and climatic requirements as well as traits characteristic for lodgepole pine (Pinus contorta Dougl. var. latifolia Engelm and the scarcity of published knowledge, we assessed the above-ground biomass of Pinus contorta in comparison to that of native Scots pine (Pinus sylvestris L. and factors affecting biomass production. Data were collected in 3 experimental trials, located in two sites in central part of Latvia: Zvirgzde and Kuldiga (56°41’ N, 24°28’ E and 57°03’ N, 21°57’ E, respectively. Trials were established with density 5000 tree ha-1, using seed material from Canada (50°08’-60°15’ N, 116°25’-132°50’ W and two Pinus contorta stands with unknown origin growing in Latvia. Results reveal that absolute dry aboveground biomass of Pinus contorta reaches 114 ± 6.4 t ha-1 at age 16 on a fertile former arable land, 48 ± 3.6 and 94 ± 9.4 t ha-1 at age 22 and 25, respectively, on a sandy forest land (Vacciniosa forest type. The biomass is significantly (p < 0.01 and considerably (more than two-fold higher than that of the native Pinus sylvestris and the productivity is similar (in fertile soils or higher (on poor soils than reported for other species in energy-wood plantations. Provenance was a significant factor affecting the above-ground biomass, and the ranking of provenances did not change significantly between different soil conditions. It provides opportunities for further improvement of productivity using selection.

  14. Anti-Microbial Evaluation of a Herbal Dental Remedy Stem Bark of Nuclea latifolia-Family Rubiaceae

    Science.gov (United States)

    Abiodun, Falodun; Igwe, A.; Osahon, Obasuyi

    The aim of the study was to evaluate the antimicrobial activity of the stem bark of Nuclea latifolia used as a dentrifice by the local populace. The crude powdered sample was evaluated for the chemical and antimicrobial effects. The methanolic and chloroform extracts were subjected to different organisms of clinical isolates Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus subtilis, Streptococcus varidans, Staphylococcus aerues, Penicillum nonatum, Candida albicans and Aspergillus niger. The Minimum Inhibitory Concentrations (MIC) were also obtained. The results of the study revealed significant antibacterial effect of the extracts. The study thus justifies the ethno medicinal use of the plant as a dental remedy.

  15. Bioprospecting the Curculigoside-Cinnamic Acid-Rich Fraction from Molineria latifolia Rhizome as a Potential Antioxidant Therapeutic Agent.

    Science.gov (United States)

    Ooi, Der Jiun; Chan, Kim Wei; Sarega, Nadarajan; Alitheen, Noorjahan Banu; Ithnin, Hairuszah; Ismail, Maznah

    2016-01-01

    Increasing evidence from both experimental and clinical studies depicts the involvement of oxidative stress in the pathogenesis of various diseases. Specifically, disruption of homeostatic redox balance in accumulated body fat mass leads to obesity-associated metabolic syndrome. Strategies for the restoration of redox balance, potentially by exploring potent plant bioactives, have thus become the focus of therapeutic intervention. The present study aimed to bioprospect the potential use of the curculigoside-cinnamic acid-rich fraction from Molineria latifolia rhizome as an antioxidant therapeutic agent. The ethyl acetate fraction (EAF) isolated from M. latifolia rhizome methanolic extract (RME) contained the highest amount of phenolic compounds, particularly curculigoside and cinnamic acid. EAF demonstrated glycation inhibitory activities in both glucose- and fructose-mediated glycation models. In addition, in vitro chemical-based and cellular-based antioxidant assays showed that EAF exhibited high antioxidant activities and a protective effect against oxidative damage in 3T3-L1 preadipocytes. Although the efficacies of individual phenolics differed depending on the structure and concentration, a correlational study revealed strong correlations between total phenolic contents and antioxidant capacities. The results concluded that enriched phenolic contents in EAF (curculigoside-cinnamic acid-rich fraction) contributed to the overall better reactivity. Our data suggest that this bioactive-rich fraction warrants therapeutic potential against oxidative stress-related disorders. PMID:27322226

  16. Bioprospecting the Curculigoside-Cinnamic Acid-Rich Fraction from Molineria latifolia Rhizome as a Potential Antioxidant Therapeutic Agent

    Directory of Open Access Journals (Sweden)

    Der Jiun Ooi

    2016-06-01

    Full Text Available Increasing evidence from both experimental and clinical studies depicts the involvement of oxidative stress in the pathogenesis of various diseases. Specifically, disruption of homeostatic redox balance in accumulated body fat mass leads to obesity-associated metabolic syndrome. Strategies for the restoration of redox balance, potentially by exploring potent plant bioactives, have thus become the focus of therapeutic intervention. The present study aimed to bioprospect the potential use of the curculigoside-cinnamic acid-rich fraction from Molineria latifolia rhizome as an antioxidant therapeutic agent. The ethyl acetate fraction (EAF isolated from M. latifolia rhizome methanolic extract (RME contained the highest amount of phenolic compounds, particularly curculigoside and cinnamic acid. EAF demonstrated glycation inhibitory activities in both glucose- and fructose-mediated glycation models. In addition, in vitro chemical-based and cellular-based antioxidant assays showed that EAF exhibited high antioxidant activities and a protective effect against oxidative damage in 3T3-L1 preadipocytes. Although the efficacies of individual phenolics differed depending on the structure and concentration, a correlational study revealed strong correlations between total phenolic contents and antioxidant capacities. The results concluded that enriched phenolic contents in EAF (curculigoside-cinnamic acid-rich fraction contributed to the overall better reactivity. Our data suggest that this bioactive-rich fraction warrants therapeutic potential against oxidative stress-related disorders.

  17. Bioprospecting the Curculigoside-Cinnamic Acid-Rich Fraction from Molineria latifolia Rhizome as a Potential Antioxidant Therapeutic Agent.

    Science.gov (United States)

    Ooi, Der Jiun; Chan, Kim Wei; Sarega, Nadarajan; Alitheen, Noorjahan Banu; Ithnin, Hairuszah; Ismail, Maznah

    2016-01-01

    Increasing evidence from both experimental and clinical studies depicts the involvement of oxidative stress in the pathogenesis of various diseases. Specifically, disruption of homeostatic redox balance in accumulated body fat mass leads to obesity-associated metabolic syndrome. Strategies for the restoration of redox balance, potentially by exploring potent plant bioactives, have thus become the focus of therapeutic intervention. The present study aimed to bioprospect the potential use of the curculigoside-cinnamic acid-rich fraction from Molineria latifolia rhizome as an antioxidant therapeutic agent. The ethyl acetate fraction (EAF) isolated from M. latifolia rhizome methanolic extract (RME) contained the highest amount of phenolic compounds, particularly curculigoside and cinnamic acid. EAF demonstrated glycation inhibitory activities in both glucose- and fructose-mediated glycation models. In addition, in vitro chemical-based and cellular-based antioxidant assays showed that EAF exhibited high antioxidant activities and a protective effect against oxidative damage in 3T3-L1 preadipocytes. Although the efficacies of individual phenolics differed depending on the structure and concentration, a correlational study revealed strong correlations between total phenolic contents and antioxidant capacities. The results concluded that enriched phenolic contents in EAF (curculigoside-cinnamic acid-rich fraction) contributed to the overall better reactivity. Our data suggest that this bioactive-rich fraction warrants therapeutic potential against oxidative stress-related disorders.

  18. Investigation on the biotrophic interaction of Ustilago esculenta on Zizania latifolia found in the Indo-Burma biodiversity hotspot.

    Science.gov (United States)

    Jose, Robinson C; Goyari, Sailendra; Louis, Bengyella; Waikhom, Sayanika D; Handique, Pratap J; Talukdar, Narayan C

    2016-09-01

    Ustilago esculenta is a uniquely flavored biotrophic smut fungus that forms a smut gall on the top internodal region of Zizania latifolia, a perennial wild rice found in the Indo-Burma biodiversity hotspot. The smut gall is an edible vegetable locally called "kambong" in Manipur, India. The life cycle of the fungus was studied in vitro and its biotrophism was observed during different stages of the plant growth starting from the bud stage to decaying stage using light, fluorescent and electron microscopy. The size of the smut gall and the number of internodes below the apical smut gall varied significantly (P < 0.05). Examination of various parts of infected plants using culture methods, microscopy and polymerase chain reaction revealed that Ustilago esculenta colonized Zizania latifolia in a non-systemic manner. Spores and fragmented hyphae of U. esculenta were present in the rhizome of infected plant throughout the year, but shoot interiors were without any fungal structures from April until September. The smut region of infected plants in early September to December were heavily sporulated with fragmented hyphae, while the nodal regions of infected plants had no spores and fragmented hyphae. Hyphae and spores were also absent in the internodes and membranes aboveground up to smut region of infected plants but were present in the old rhizomes. PMID:27334294

  19. Biological control of golden apple snail, Pomacea canaliculata by Chinese soft-shelled turtle, Pelodiscus sinensis in the wild rice, Zizania latifolia field

    Directory of Open Access Journals (Sweden)

    Shengzhang Dong

    2012-04-01

    Full Text Available The wild rice, Zizania latifolia Turcz, used to be one of the important aquatic vegetables cultivated in China. Recently, the golden apple snail - GAS (Pomacea canaliculata (Lamarck was found to be a major invasive pest attacking Z. latifolia. To control efficiently GAS, predation by the Chinese soft-shelled turtles (Pelodiscus sinensis on GAS was evaluated in laboratory and field trials. P. sinensis had a strong predatory capacity and selectivity for GAS both in laboratory and field conditions. All the sizes of P. sinensis prefer to capture smaller snails. The optimum number of P. sinensis released in Z. latifolia field was dependent on the density of over-wintered GAS, and varied between 30 and 50 turtles per 666.7 m². The number of GAS declined in the fields with turtles as compared to turtle-free field. A pattern of releasing P. sinensis in Z. latifolia fields was developed and widely adopted by farmers because of much more benefit besides biologically controlling GAS.

  20. 蒲菜的保鲜和加工研究进展%Research Process on Preservation and Technique of Typha Latifolia

    Institute of Scientific and Technical Information of China (English)

    田其英

    2014-01-01

    The respiration,PPO activity and the fibrin course of Typha latifolia after picking,the preservation technique effect on living Typha latifolia including temperature,controlled atmosphere,coating and chemical reagent,as well as the research results of product processing technology of Typha latifolia were summarized in this article.Then the characteristic of a variety of different preservation technique was contrastd and analyzed ,the development trend about preservation and processing technique of Typha latifolia has been looked forward to.%本文归纳总结了蒲菜采后呼吸作用、多酚氧化酶(PPO)活性和纤维化进程,温度、气调、涂膜、化学保鲜技术在蒲菜保鲜中的应用效果以及蒲菜产品加工技术研究成果,并对比分析了各种保鲜技术的特点,展望了蒲菜保鲜和加工技术发展趋势。

  1. 中国发现新的检疫性杂草宽叶酢浆草%Quarantine Weed Oxalis latifolia Firstly Found in China

    Institute of Scientific and Technical Information of China (English)

    汤东生; 刘萍; 傅杨

    2013-01-01

    宽叶酢浆草(Oxalis latifolia)是2010年在云南省昆明市新发现的一种入侵性强危害重的外来植物.为了解其危害特点,预测其将来对中国农业生产的影响及制定有效的控制措施,综述了该物种生物生态学特性和国外的危害和防治情况.通过GBIF软件的模拟分析了宽叶酢浆草在中国的入侵定殖风险程度.综合以上分析,提出了深入开展宽叶酢浆草的生态学和危害规律研究的必要性,与防范和化解宽叶酢浆草在中国的扩展蔓延的重要意义.%Oxalis latifolia was a new quarantine invasive plant found in Kunming of Yunnan Province in 2010. The weed has caused serious damage to production. To understand damage pattern of 0. latifolia, forecast its influence on agricultural production of China, and make effective solutions to control the weed, the present study was carried out to review biological and ecological characteristics and the condition of damage and control in overseas. Moreover, the invasive and colonization risk of O. latifolia in China was analyzed by the software of GBIF. Based on the above analysis, the paper purposed the necessary to launch ecological and damage patterns of 0. latifolia, the significance to prevent and resolve its spread in China.

  2. Co-hyperaccumulative characteristics of lead, zinc and cadmium by Silene viscidula Franch%滇白前(Silene viscidula)对铅、锌、镉的共超富集特征

    Institute of Scientific and Technical Information of China (English)

    肖青青; 王宏镔; 王海娟; 叶志鸿

    2009-01-01

    为了寻找新的重金属超富集植物特别是多金属共超富集植物,调查测定了云南兰坪铅锌矿区北厂矿段生长的8种植物及其根区土壤的重金属质量分数,以及土壤基本理化性质.结果表明:研究区土壤中磷和钾质量分数较低,总氮、总磷、总钾、铵态氮、硝态氮、速效磷分别占土壤干质量的0.2%、0.03%、0.52%、0.0029%、0.000 12%、0.00068%;有机质质量分数平均为4.81%,pH值平均为6.79,电导率变化范围为11.4~140-3μs·cm-1.该矿区土壤中锌(Zn)、铅(Pb)、镉(Cd)、铜(Cu)的质量分数平均值分别为(38 178±23 870)、(18 671±10 143)、(438±345)、(159±:82)mg·kg>-1,除Cu外均超过国家土壤环境质量(GB 15618-1995)三级标准.8种植物地上部Zn、Pb、Cd、Cu质量分数范围分别为271~17 986、51~5 430、1~617、2~26mg·kg-1,尤以滇白前(Silene viscidula Franch)地上部Zn、Pb、Cd质量分数为最高.进一步采集38个滇白前样本对其重金属富集特征进行深人调查,表明其地上部中含zn、Pb和Cd平均为(11 043±3 537)、(1 546±1 044)和(391±196)mg·kg-1,富集系数(地上部和土壤金属质量分数之比)分别为0.35、0.08和1.05,转运系数(地上部和根中金属质量分数之比)均超过1,均值分别为8.21、3.90和8.36.野外调查数据表明,滇白前是一种Pb/Zn/Cd共超富集植物.滇白前对Zn、Pb富集系数小于1,主要是由于其对应土壤中Zn、Pb质量分数太高(平均分别为(45 778±32 819)、(22 512±13 613)mg·kg-1)所致.

  3. Evaluation of Compatibility between Beetle-Killed Lodgepole Pine (Pinus Contorta var. Latifolia Wood with Portland Cement

    Directory of Open Access Journals (Sweden)

    Ian D. Hartley

    2010-12-01

    Full Text Available The compatibility of wood from mountain pine beetle (Dendroctonus ponderosa killed lodgepole pine (Pinus contorta var. latifolia with Portland cement was investigated based on time-since-death as a quantitative estimator, and the presence of blue-stained sapwood, brown rot, or white rot as qualitative indicators. The exothermic behavior of cement hydration, maximum heat rate, time to reach this maximum, and total heat released within a 3.5–24 h interval were used for defining a new wood-cement compatibility index (CX. CX was developed and accounted for large discrepancies in assessing wood-cement compatibility compared to the previous methods. Using CX, no significant differences were found between fresh or beetle-killed wood with respect to the suitability for cement; except for the white rot samples which reached or exceeded the levels of incompatibility. An outstanding physicochemical behavior was also found for blue-stained sapwood and cement, producing significantly higher compatibility indices.

  4. The role of sand, marble chips and Typha latifolia in domestic wastewater treatment - a column study on constructed wetlands.

    Science.gov (United States)

    Kadaverugu, Rakesh; Shingare, Rita P; Raghunathan, Karthik; Juwarkar, Asha A; Thawale, Prashant R; Singh, Sanjeev K

    2016-10-01

    The relative importance of sand, marble chips and wetland plant Typha latifolia is evaluated in constructed wetlands (CWs) for the treatment of domestic wastewater intended for reuse in agriculture. The prototype CWs for the experiments are realized in polyvinyl chloride columns, which are grouped into four treatments, viz. sand (hydraulic retention time in the columns. The statistical analysis suggests that the main effects of sand and cattail are found to be significant (p hydraulic conductivity is also reported for all the treatments. Thus, the findings of this study elucidate the role of low-cost and easily available filter media and it will guide the environmental practitioners in designing cost-effective CWs for wastewater treatment. PMID:26878342

  5. Root-zone acidity and nitrogen source affects Typha latifolia L. growth and uptake kinetics of ammonium and nitrate.

    Science.gov (United States)

    Brix, Hans; Dyhr-Jensen, Kirsten; Lorenzen, Bent

    2002-12-01

    The NH(4)(+) and NO(3)(-) uptake kinetics by Typha latifolia L. were studied after prolonged hydroponics growth at constant pH 3.5, 5.0, 6.5 or 7.0 and with NH(4)(+) or NO(3)(-) as the sole N-source. In addition, the effects of pH and N source on H(+) extrusion and adenine nucleotide content were examined. Typha latifolia was able to grow with both N sources at near neutral pH levels, but the plants had higher relative growth rates, higher tissue concentrations of the major nutrients, higher contents of adenine nucleotides, and higher affinity for uptake of inorganic nitrogen when grown on NH(4)(+). Growth almost completely stopped at pH 3.5, irrespective of N source, probably as a consequence of pH effects on plasma membrane integrity and H(+) influx into the root cells. Tissue concentrations of the major nutrients and adenine nucleotides were severely reduced at low pH, and the uptake capacity for inorganic nitrogen was low, and more so for NO(3)(-)-fed than for NH(4)(+)-fed plants. The maximum uptake rate, V(max), was highest for NH(4)(+) at pH 6.5 (30.9 micro mol h(-1) g(-1) root dry weight) and for NO(3)(-) at pH 5.0 (31.7 micro mol h(-1) g(-1) root dry weight), and less than 10% of these values at pH 3.5. The affinity for uptake as estimated by the half saturation constant, K((1/2)), was lowest at low pH for NH(4)(+) and at high pH for NO(3)(-). The changes in V(max) and K((1/2)) were thus consistent with the theory of increasing competition between cations and H(+) at low pH and between anions and OH(-) at high pH. C(min) was independent of pH, but slightly higher for NO(3)(-) than for NH(4)(+) (C(min)(NH(4)(+)) approximately 0.8 mmol m(-3); C(min)(NO(3)(-)) approximately 2.8 mmol m(-3)). The growth inhibition at low pH was probably due to a reduced nutrient uptake and a consequential limitation of growth by nutrient stress. Typha latifolia seems to be well adapted to growth in wetland soils where NH(4)(+) is the prevailing nitrogen compound, but very low p

  6. Spotlight census of the red fox (Vulpes vulpes and the domestic cat (Felis catus in three sample areas of the Marches region (Central Italy / Censimento notturno di Volpe (Vulpes vulpes e di Gatto domestico (Felis catus in tre aree campione delle Marche

    Directory of Open Access Journals (Sweden)

    Massimo Pandolfi

    1991-07-01

    Full Text Available Abstract In order to evaluate the density of the red fox and of the domestic cat, 55 transects were made from 1986 to 1989 using spotlight census method in three sample areas. The mean density of foxes agreed substantially with its biological cycle and the hightes values (2.01 foxes/km² in spring and 4.3 foxes/km² in winter were recorded in the study area with the better natural characteristics. Foxes selected the shrub woodland (macchia all year round, the inhabited area in spring. The domestic cat was widely spread and abundant, and selected especially inhabited areas where the density varied from 4.27 cats/km² (in winter to 12.42 cat/km² (in spring. Riassunto Dal 1986 al 1989, con il metodo dei percorsi notturni con fari, sono stati effettuati complessivamente 55 conteggi in tre aree campione per valutare la densità della Volpe (Vulpes vulpes e del Gatto domestico (Felis catus nonché le loro preferenze ambientali limitatamente ad una zona campione. Per la Volpe le densità medie rilevate sono sostanzialmente in accordo con il ciclo biologico della specie e quelle più elevate (2,O1 volpi/km² in primavera e 4,3 volpi/km² in inverno sono state registrate nella zona campione con maggior presenza di boschi ed aree incolte. La Volpe seleziona le zone con vegetazione "di macchia" in ogni periodo dell'anno, e le aree abitate in primavera. Per il Gatto domestico le densità rilevate evidenziano la presenza di una diffusa ed abbondante popolazione. La specie mostra una spiccata preferenza per le aree abitate dove raggiunge densità di 4,27 individui/km² e 12,42 individui/km² in inverno e primavera rispettivamente.

  7. Studio preliminare su un campione di soggetti con diagnosi di disturbo di personalità borderline ricoverati presso il Day Hospital Psichiatrico della Struttura di Psichiatria S.P.D.C. dell’Azienda Ospedaliera “SS. Antonio e Biagio e Cesare Arrigo” di Alessandria

    OpenAIRE

    Bergonzini C.;; Veglia F.;; Bertamini D.;; Piantato E.; Prelati M.; Raffaelli S.;

    2013-01-01

    Obiettivi: Il progetto di ricerca è finalizzato all’osservazione dell’attività del day hospital psichiatrico e alla valutazione dell’efficacia del trattamento su un gruppo di pazienti con diagnosi di Disturbo Borderline di Personalità. Metodologia: Il campione è costituito da un gruppo di 5 pazienti con diagnosi di DBP in terapia presso il day hospital, che saranno seguiti per 6 mesi sia con terapia farmacologica che con una psicoterapia che segue le linee guida del Trattamento basato sulla M...

  8. The fate of arsenic, cadmium and lead in Typha latifolia: A case study on the applicability of micro-PIXE in plant ionomics

    Energy Technology Data Exchange (ETDEWEB)

    Lyubenova, Lyudmila [Helmholtz Zentrum München, German Research Centre for Environmental Health, Department of Environmental Sciences, Research Unit Microbe–Plant Interactions, Ingolstädter Landstr. 1, D-85764 Neuherberg (Germany); Pongrac, Paula; Vogel-Mikuš, Katarina [University of Ljubljana, Biotechnical Faculty, Department of Biology, Večna pot 111, SI-1000 Ljubljana (Slovenia); Mezek, Gašper Kukec; Vavpetič, Primož; Grlj, Nataša [Jožef Stefan Institute, Jamova 39, SI-1000 Ljubljana (Slovenia); Regvar, Marjana [University of Ljubljana, Biotechnical Faculty, Department of Biology, Večna pot 111, SI-1000 Ljubljana (Slovenia); Pelicon, Primož [Jožef Stefan Institute, Jamova 39, SI-1000 Ljubljana (Slovenia); Schröder, Peter, E-mail: peter.schroeder@helmholtz-muenchen.de [Helmholtz Zentrum München, German Research Centre for Environmental Health, Department of Environmental Sciences, Research Unit Microbe–Plant Interactions, Ingolstädter Landstr. 1, D-85764 Neuherberg (Germany)

    2013-03-15

    Highlights: ► Uptake, accumulation and distribution of multi-elemental pollution. ► Quantitative analysis of the spatal distribution of nutrients in roots and rhizomes. ► Typha latifolia – plant species important for phytoremediation of hazardous xenobiotics. ► Low amounts of Cd and Pb in the inner tissues of roots and rhizomes. ► Predominantly As was found within the vascular tissue – high mobility of the element. -- Abstract: Understanding the uptake, accumulation and distribution of toxic elements in plants is crucial to the design of effective phytoremediation strategies, especially in the case of complex multi-element pollution. Using micro-proton induced X-ray emission, the spatial distribution of Na, Mg, Al, Si, P, S, Cl, K, Ca, Mn, Fe, Zn, As, Br, Rb, Sr, Cd and Pb have been quantitatively resolved in roots and rhizomes of an obligate wetland plant species, Typha latifolia, treated with a mixture of 100 μM each of As, Cd and Pb, together. The highest concentrations of As, Cd and Pb were found in the roots of the T. latifolia, with tissue-specific distributions. The As was detected in the root rhizodermis, and in the rhizome the majority of the As was within the vascular tissues, which indicates the high mobility of As within T. latifolia. The Cd was detected in the root exodermis, and in the vascular bundle and epidermis of the rhizome. The highest Pb concentrations were detected in the root rhizodermis and exodermis, and in the epidermis of the rhizome. These data represent an essential step in the resolution of fundamental questions in plant ionomics.

  9. Identification of Abiotic and Biotic Factors Causing Deterioration During Storage and Development of Storage Techniques for Mahua (Madhuca indica Syn. Bassia latifolia) flowers

    OpenAIRE

    Basanta Kumar Das

    2010-01-01

    Mahua (Madhuca indica syn. Bassia latifolia) flowers, occupy an important position in the life of the tribal in many parts of India. Particularly, the flowers of the plant are sugar rich and in certain cases it is the only source of livelihood for those people. However, its nutrient quality deteriorates during the postharvest storage and thus, poses a serious problem of adequate storage. In order to determine the cause of spoilage and to develop the measures to check it, collected flowers wer...

  10. Distribución y uso tradicional de Sagittaria macrophylla Zucc. y S. latifolia Willd. en el Estado de México

    Directory of Open Access Journals (Sweden)

    Carmen Zepeda Gómez

    2005-01-01

    Full Text Available Sagittaria macrophylla y S. latifolia son plantas acuáticas emergentes que crecen en las orillas y zonas poco profundas de los cuerpos de agua limpios y de poca corriente. La primera es endémica de México, su distribución se restringe a la región del río Lerma y valle de México y está en peligro de extinción. Sagittarial latifolia se distribuye desde Canadá hasta el noroeste de Sudamérica. En México se localiza al menos en 11 estados. El análisis de la distribución de ambas especies muestra una disminución en el Estado de México; los factores que aparentemente están contribuyendo a este fenómeno son, en general, la explosión demográfica, la pérdida del hábitat y la sobreexplotación. No había registros del uso de S. latifolia en México, actualmente se reconoce que sus tubérculos o ‘papas de agua’ tienen importancia económica en la región del río Lerma. El uso tradicional de ambas especies indica que podrían ser una alternativa más en la dieta humana y animal.

  11. Leaf gas exchange and fluorescence of Phillyrea latifolia, Pistacia lentiscus and Quercus ilex saplings in severe drought and high temperature conditions

    International Nuclear Information System (INIS)

    Saplings of Phillyrea latifolia, Pistacia lentiscus and Quercus ilex were withheld watering for 7 days, followed by reirrigation. Incident photosynthetic photon flux density (PPFD), leaf temperature, net photosynthetic rates, stomatal conductance, and photochemical efficiency of the photosystem II (ΔF/F'm) were measured three times during the day. The watered plants had higher photosynthetic rates, stomatal conductances, ΔF/F'm and ETR than non-watered plants. However, watered plants were mildly water stressed as shown by low ratio of variable to maximal fluorescence (Fv/Fm) and high non-photochemical fluorescence quenching (qN). Their ΔF/F′m was low in the morning and increased in the evening, following the variations in PPFD. Watered plants of Q. ilex had lower photosynthetic activity, stomatal conductance and photosynthetic radiation use efficiency than Ph. latifolia and P. lentiscus, and, conversely, reached the highest ΔF/F′m and ETR. This seems to indicate a different relationship between photosynthetic activity and electron transport rate in Q. ilex compared to the other two species. Ph. latifolia and P. lentiscus appeared to be better adapted to severe drought than Q. ilex. (author)

  12. Vermischte Notizen zur Benennung hessischer Pflanzen : dritter Nachtrag zum "Namensverzeichnis zur Flora der Farn- und Samenpflanzen Hessens"

    OpenAIRE

    Buttler, Karl Peter

    1998-01-01

    Die seit dem zweiten Nachtrag (Januar 1997) bekannt gewordenen Ergänzungen und Verbesserungen zum "Namensverzeichnis" sind im dritten Nachtrag zusammengefaßt. Angefügt sind Anmerkungen zur Nomenklatur von Armeria vulgaris/A. elongata, Cichorium intybus, Pyrus pyraster, Silene dioica und S. latifolia subsp. alba, Silene viscaria, Stellaria palustris. Neue Kombination: Bassia scoparia var. subvillosa (Moquin-Tandon) Buttler.

  13. Genomic Rearrangement in Endogenous Long Terminal Repeat Retrotransposons of Rice Lines Introgressed by Wild Rice (Zizania latifolia Griseb.)

    Institute of Scientific and Technical Information of China (English)

    Ye SHEN; Xiu-Yun LIN; Xiao-Hui SHAN; Chun-Jing LIN; Fang-Pu HAN; Jin-Song PANG; Bao LIU

    2005-01-01

    Stochastic introgression of alien DNA may impose a genomic stress to the recipient genome.Herein, we report that apparent de novo genomic rearrangements in 10 of 13 selected endogenous, low-copy, and potentially active long terminal repeat (LTR) retrotransposons occurred in one or more of threerice lines studied that were introgressed by wild rice (Zizania latifolia Griseb.). For nine retrotransposons inwhich both the reverse-transcriptase (RT) region and the LTR region were available, largely concordantrearrangements occurred at both regions in five elements and at the RT region only in the remaining fourelements. A marked proportion of the genomic changes was shared by two or all three introgression linesthat were derived from a single F1 plant. This indicates that most of the genomic changes occurred at earlydevelopmental stages of the F1 somatic cells, which then gave rise to germline cells, and, hence, ensuredinheritance of the changes to later generations. Possible causes and potential implications of the introgres-sion-induced genomic rearrangements in LTR retrotransposons are discussed in the context of plant ge-nome evolution and breeding.

  14. Evaluation of Anti-Inflammatory Activity of Citrus latifolia Tanaka Essential Oil and Limonene in Experimental Mouse Models

    Directory of Open Access Journals (Sweden)

    Raquel Kummer

    2013-01-01

    Full Text Available The genus Citrus (Rutaceae includes several species of plants that produce some of the most cultivated fruits in the world, providing an appreciable content of essential oil. In folk medicine, they are used as a cholagogue, antipyretic, anti-inflammatory, sedative, and antitoxic effects. Lemon essential oil has been used since ancient times for its antiseptic, carminative, diuretic, and eupeptic effects. In this study, we investigated the anti-inflammatory activity of Citrus latifolia Tanaka essential oil (CLEO and its main constituent LIM. In the cell viability assay, CLEO and LIM (3, 10, 30, and 90 μg/mL had low cytotoxicity. In zymosan-induced peritonitis, LIM (500 mg/kg decreased the infiltration of peritoneal exudate leukocytes and decreased the number of polymorphonuclear leukocytes. In vitro chemotaxis revealed that CLEO and LIM (1, 3, and 10 µg/mL promoted a significant reduction of neutrophil migration toward fMLP and LTB4. LIM (500 mg/kg also reduced TNF-α levels but did not alter IL-10 levels in the peritoneal exudate. In conclusion, this study showed that LIM isolated from CLEO had potential anti-inflammatory effects, likely by inhibiting proinflammatory mediators present in inflammatory exudate and leukocyte chemotaxis.

  15. Preliminary Screening of Antioxidant and Antibacterial Activities and Establishment of an Efficient Callus Induction in Curculigo latifolia Dryand (Lemba

    Directory of Open Access Journals (Sweden)

    Reza Farzinebrahimi

    2016-01-01

    Full Text Available Leaf, seed, and tuber explants of C. latifolia were inoculated on MS medium supplemented with various concentrations of BAP and IBA, alone or in combinations, to achieve in vitro plant regeneration. Subsequently, antioxidant and antibacterial activities were determined from in vitro and in vivo plant developed. No response was observed from seed culture on MS media with various concentrations of PGRs. The highest percentage of callus was observed on tuber explants (94% and leaf explants (89% when cultured on MS media supplemented with IBA in combination with BAP. A maximum of 88% shoots per tuber explant, with a mean number of shoots (8.8±1.0, were obtained on MS medium supplemented with combinations of BAP and IBA (2.5 mg L−1. The best root induction (92% and mean number (7.6±0.5 from tuber explants were recorded on 2.5 mg L−1 IBA alone supplemented to MS medium. The higher antioxidant content (80% was observed from in vivo tuber. However, tuber part from the intact plant showed higher inhibition zone in antibacterial activity compared to other in vitro and in vivo tested parts.

  16. Comparative study of bio-ethanol production from mahula (Madhuca latifolia L.) flowers by Saccharomyces cerevisiae and Zymomonas mobilis

    Energy Technology Data Exchange (ETDEWEB)

    Behera, Shuvashish; Mohanty, Rama Chandra [Department of Botany, Utkal University, Vanivihar, Bhubaneswar 751004, Orissa (India); Ray, Ramesh Chandra [Microbiology Laboratory, Central Tuber Crops Research Institute (Regional Centre), Bhubaneswar 751019, Orissa (India)

    2010-07-15

    Mahula (Madhuca latifolia L.) flower is a suitable alternative cheaper carbohydrate source for production of bio-ethanol. Recent production of bio-ethanol by microbial fermentation as an alternative energy source has renewed research interest because of the increase in the fuel price. Saccharomyces cerevisiae (yeast) and Zymomonas mobilis (bacteria) are two most widely used microorganisms for ethanol production. In this study, experiments were carried out to compare the potential of the yeast S. cerevisiae (CTCRI strain) with the bacterium Z. mobilis (MTCC 92) for ethanol fermentation from mahula flowers. The ethanol production after 96 h fermentation was 149 and 122.9 g kg{sup -1} flowers using free cells of S. cerevisiae and Z. mobilis, respectively. The S. cerevisiae strain showed 21.2% more final ethanol production in comparison to Z. mobilis. Ethanol yield (Yx/s), volumetric product productivity (Qp), sugar to ethanol conversion rate (%) and microbial biomass concentration (X) obtained by S. cerevisiae were found to be 5.2%, 21.1%, 5.27% and 134% higher than Z. mobilis, respectively after 96 h of fermentation. (author)

  17. Comparative study of bio-ethanol production from mahula (Madhuca latifolia L.) flowers by Saccharomyces cerevisiae and Zymomonas mobilis

    International Nuclear Information System (INIS)

    Mahula (Madhuca latifolia L.) flower is a suitable alternative cheaper carbohydrate source for production of bio-ethanol. Recent production of bio-ethanol by microbial fermentation as an alternative energy source has renewed research interest because of the increase in the fuel price. Saccharomyces cerevisiae (yeast) and Zymomonas mobilis (bacteria) are two most widely used microorganisms for ethanol production. In this study, experiments were carried out to compare the potential of the yeast S. cerevisiae (CTCRI strain) with the bacterium Z. mobilis (MTCC 92) for ethanol fermentation from mahula flowers. The ethanol production after 96 h fermentation was 149 and 122.9 g kg-1 flowers using free cells of S. cerevisiae and Z. mobilis, respectively. The S. cerevisiae strain showed 21.2% more final ethanol production in comparison to Z. mobilis. Ethanol yield (Yx/s), volumetric product productivity (Qp), sugar to ethanol conversion rate (%) and microbial biomass concentration (X) obtained by S. cerevisiae were found to be 5.2%, 21.1%, 5.27% and 134% higher than Z. mobilis, respectively after 96 h of fermentation. (author)

  18. Analysis of Active Components and Proteomics of Chinese Wild Rice (Zizania latifolia (Griseb) Turcz) and Indica Rice (Nagina22).

    Science.gov (United States)

    Jiang, Ming-Xia; Zhai, Li-Jie; Yang, Hua; Zhai, Shu-Menghui; Zhai, Cheng-Kai

    2016-08-01

    The ancient Chinese wild rice (Zizania latifolia (Griseb) Turcz) (CWR) has valuable biological and medicinal functions. To assess the advantages lost in modern cultivated rice after domestication, we compared the composition of bioactive compounds and the results of proteomic analysis with those of Indica rice (N22). We used routine methods to determine the protein, total dietary fiber, amino acid, mineral substance, plant secondary metabolites, and amino acid composition of CWR and N22. The protein and mineral contents of CWR were two times that of N22, and the levels of calcium, potassium, magnesium, chromium, iron, and zinc were significantly higher than those of N22 (P sterols, respectively. The amino acid score of CWR was 66.6, which was significantly higher than N22. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) indicated that the main seed proteins of CWR were glutelins, including both acid and alkaline subunits, which were approximately twice those of N22. To investigate the differences in protein profiles between CWR and N22, we conducted two-dimensional electrophoresis (2-DE) analysis of the total proteins in the seeds of the two rice species. 2-DE gels revealed 19 differentially expressed proteins. Information obtained from peptide mass fingerprinting indicates that glutelin precursor caffeoyl coenzyme A (CoA) O-methyltransferase and putative bithoraxoid-like protein can provide good gene sources for improving rice quality. PMID:27533651

  19. Disease status and population origin effects on floral scent: potential consequences for oviposition and fruit predation in a complex interaction between a plant, fungus, and noctuid moth

    NARCIS (Netherlands)

    Dötterl, S.; Jürgens, A.; Wolfe, L.M.; Biere, A.

    2009-01-01

    In the Silene latifolia-Hadena bicruris nursery pollination system, the Hadena moth is both pollinator and seed predator of its host plant. Floral scent, which differs among S. latifolia individuals and populations, is important for adult Hadena to locate its host. However, the success of moth larva

  20. 馉饳菜生物学特性的研究%Study on the Biologic Characteristics of Silene conoidea L.

    Institute of Scientific and Technical Information of China (English)

    徐恒戬; 李翠香

    2011-01-01

    Wild vegetable was a kind of satisfactory food, but the resource was deficient because of weed killer and predatory collection.Silene conoidea L.was a satisfactory wild vegetable in the locality.In this paper, its characteristics of growth and development and seed germination were investigated.The results showed that the seeds germinated quickly below 15 ℃ ,and grew well before spring festival.The vegetable product was collected to the end of March.The seed was short-lived seed.Only did the seed collected in the same year germinated well, and old seeds germination rate was very low.The new seed germinated evenly in 3 - 5 days and not affected by light.%麦瓶草幼苗是当地人们喜食的野菜,俗称馉饳菜.本试验对其生长发育性状和种子发芽特性进行了研究,以期为麦瓶草的栽培提供依据.结果表明,麦瓶草种子在低于15℃时播种,可以迅速发芽,在年前形成幼苗,年后为产品形成期,至3月底采收结束.麦瓶草种子寿命较短,为短命种子,只有当年采收的种子才萌发良好,陈种子发芽率极低.种子发芽整齐度高,萌发期3~5 d,光照对发芽过程没有影响,为中光性.

  1. Fractionation of stable zinc isotopes in the field-grown zinc hyperaccumulator Noccaea caerulescens and the zinc-tolerant plant Silene vulgaris.

    Science.gov (United States)

    Tang, Ye-Tao; Cloquet, Christophe; Sterckeman, Thibault; Echevarria, Guillaume; Carignan, Jean; Qiu, Rong-Liang; Morel, Jean-Louis

    2012-09-18

    Stable Zn isotope signatures offer a potential tool for tracing Zn uptake and transfer mechanisms within plant-soil systems. Zinc isotopic compositions were determined in the Zn hyperaccumulator Noccaea caerulescens collected at a Zn-contaminated site (Viviez), a serpentine site (Vosges), and a noncontaminated site (Sainte Eulalie) in France. Meanwhile, a Zn-tolerant plant ( Silene vulgaris ) was also collected at Viviez for comparison. While δ(66)Zn was substantially differentiated among N. caerulescens from the three localities, they all exhibited an enrichment in heavy Zn isotopes of 0.40-0.72‰ from soil to root, followed by a depletion in heavy Zn from root to shoot (-0.10 to -0.50‰). The enrichment of heavy Zn in roots is ascribed to the transport systems responsible for Zn absorption into root symplast and root-to-shoot translocation, while the depletion in heavy Zn in shoots is likely to be mediated by a diffusive process and an efficient translocation driven by energy-required transporters (e.g., NcHMA4). The mass balance yielded a bulk Zn isotopic composition between plant and soil (Δ(66)Zn(plant-soil)) of -0.01‰ to 0.63‰ in N. caerulescens , indicative of high- and/or low-affinity transport systems operating in the three ecotypes. In S. vulgaris , however, there was no significant isotope fractionation between whole plant and rhizosphere soil and between root and shoot, suggesting that this species appears to have a particular Zn homeostasis. We confirm that quantifying stable Zn isotopes is useful for understanding Zn accumulation mechanisms in plants. PMID:22891730

  2. 茭白对铬的吸收积累及其亚细胞分布%Cr Accumulation and Sub-cellular Distribution in Zizania latifolia

    Institute of Scientific and Technical Information of China (English)

    朱四喜; 王凤友; 刘文; 史云勋; 杨秀琴; 吴云杰

    2014-01-01

    为茭白在水体铬污染修复的运用提供科学依据,在模拟小型湿地试验系统中,用不同浓度(0,5 mg/L,10 mg/L,20 mg/L,40 mg/L,60 mg/L)的含铬(Ⅵ)废水进行污染胁迫,研究在不同 Cr(Ⅵ)浓度处理下,茭白的生长状况、各器官中铬的含量与积累量及其亚细胞的分布特征。结果表明,不同铬浓度处理下,茭白各器官生物量表现为根>叶>茎,且在铬胁迫浓度为20 mg/L时,株高、根长、生物量均较大;相同 Cr浓度胁迫下,不同器官中铬含量不同,总趋势为茎>根>叶;茭白对铬的耐性指数与转运系数均较高,且在20 mg/L时最高;茭白各器官亚细胞中铬分布总趋势为胞液>细胞壁>细胞器。结论:茭白对低浓度(0~20 mg/L)水体铬污染具有较强的积累力、转运力和耐受力,在水体铬污染植物修复中具有一定的应用价值。%Z.latifolia plants were cultivated in waste water with different Cr concentrations to study their growth status,Cr accumulation in different organs and sub-cellular distribution characteristics under different Cr stress and to provide the scientific basis for phytoremediation of;Z.latifolia in Cr pollution of water.The biomass of different organs was root leaf stem under different Cr concentrations and Z. latifolia had higher height,root length and biomass when Cr concentration was 20mg/L.The general trend of Cr content and Cr accumulation in different organs was stem root leaf and root stem leaf under the same Cr concentration.Z.latifolia had higher Cr tolerance index and transfer coefficient and the Cr tolerance index and transfer coefficient was up to the highest when Cr concentration was 20mg/L.Cr was distributed in cell wall and cytosol mainly and the general trend of Cr distribution in sub-cellular level was cytosol cell wall organelle.In conclusion,Z.latifolia with the strong accumulation ability,transfer ability and tolerance

  3. The composition and depth of green roof substrates affect the growth of Silene vulgaris and Lagurus ovatus species and the C and N sequestration under two irrigation conditions.

    Science.gov (United States)

    Ondoño, S; Martínez-Sánchez, J J; Moreno, J L

    2016-01-15

    Extensive green roofs are used to increase the surface area covered by vegetation in big cities, thereby reducing the urban heat-island effect, promoting CO2 sequestration, and increasing biodiversity and urban-wildlife habitats. In Mediterranean semi-arid regions, the deficiency of water necessitates the use in these roofs of overall native plants which are more adapted to drought than other species. However, such endemic plants have been used scarcely in green roofs. For this purpose, we tested two different substrates with two depths (5 and 10 cm), in order to study their suitability with regard to adequate plant development under Mediterranean conditions. A compost-soil-bricks (CSB) (1:1:3; v:v:v) mixture and another made up of compost and bricks (CB) (1:4; v:v) were arranged in two depths (5 and 10 cm), in cultivation tables. Silene vulgaris (Moench) Garcke and Lagurus ovatus L. seeds were sown in each substrate. These experimental units were subjected, on the one hand, to irrigation at 40% of the registered evapotranspiration values (ET0) and, on the other, to drought conditions, during a nine-month trial. Physichochemical and microbiological substrate characteristics were studied, along with the physiological and nutritional status of the plants. We obtained significantly greater plant coverage in CSB at 10 cm, especially for L. ovatus (80-90%), as well as a better physiological status, especially in S. vulgaris (SPAD values of 50-60), under irrigation, whereas neither species could grow in the absence of water. The carbon and nitrogen fixation by the substrate and the aboveground biomass were also higher in CSB at 10 cm, especially under L. ovatus - in which 1.32 kg C m(-2) and 209 g N m(-2) were fixed throughout the experiment. Besides, the enzymatic and biochemical parameters assayed showed that microbial activity and nutrient cycling, which fulfill a key role for plant development, were higher in CSB. Therefore, irrigation of 40% can

  4. The composition and depth of green roof substrates affect the growth of Silene vulgaris and Lagurus ovatus species and the C and N sequestration under two irrigation conditions.

    Science.gov (United States)

    Ondoño, S; Martínez-Sánchez, J J; Moreno, J L

    2016-01-15

    Extensive green roofs are used to increase the surface area covered by vegetation in big cities, thereby reducing the urban heat-island effect, promoting CO2 sequestration, and increasing biodiversity and urban-wildlife habitats. In Mediterranean semi-arid regions, the deficiency of water necessitates the use in these roofs of overall native plants which are more adapted to drought than other species. However, such endemic plants have been used scarcely in green roofs. For this purpose, we tested two different substrates with two depths (5 and 10 cm), in order to study their suitability with regard to adequate plant development under Mediterranean conditions. A compost-soil-bricks (CSB) (1:1:3; v:v:v) mixture and another made up of compost and bricks (CB) (1:4; v:v) were arranged in two depths (5 and 10 cm), in cultivation tables. Silene vulgaris (Moench) Garcke and Lagurus ovatus L. seeds were sown in each substrate. These experimental units were subjected, on the one hand, to irrigation at 40% of the registered evapotranspiration values (ET0) and, on the other, to drought conditions, during a nine-month trial. Physichochemical and microbiological substrate characteristics were studied, along with the physiological and nutritional status of the plants. We obtained significantly greater plant coverage in CSB at 10 cm, especially for L. ovatus (80-90%), as well as a better physiological status, especially in S. vulgaris (SPAD values of 50-60), under irrigation, whereas neither species could grow in the absence of water. The carbon and nitrogen fixation by the substrate and the aboveground biomass were also higher in CSB at 10 cm, especially under L. ovatus - in which 1.32 kg C m(-2) and 209 g N m(-2) were fixed throughout the experiment. Besides, the enzymatic and biochemical parameters assayed showed that microbial activity and nutrient cycling, which fulfill a key role for plant development, were higher in CSB. Therefore, irrigation of 40% can

  5. Study on Production Technology of Typha latifolia L. Juice Beverage%蒲菜汁饮料生产工艺研究

    Institute of Scientific and Technical Information of China (English)

    董静; 杨用钊; 周美玲

    2011-01-01

    [目的]研究蒲菜鲜汁加工成新型绿色保健饮料的最佳工艺.[方法]通过单因素和正交试验,研究蒲菜汁饮料的最佳配方和工艺条件.[结果]最佳配方为:料液比15%.蔗糖4%、柠檬酸0.09%、明胶0.10%.混合物料经20 MPa均质,90~100℃杀菌10 min,最终获得乳白色、无沉淀、口感佳、具有浓郁蒲菜香气的饮料.通过感官指标、理化指标、微生物指标进行检测得到合格的蒲莱汁饮料,保存期可达6个月以上.[结论]该工艺条件下生产的蒲菜汁饮料口感好、营养丰富、稳定性强.%[ Objective ] The aim was to study production technology of Typha latifolia L. juice beverage. [ Method ] A new green-health beverage was made from fresh juice of Typha latifolia L. Through single-factor and orthogonal experiments, the optimum formula and processing conditions for the Typha latifolia L. juice beverage were investigated. [ Result ] The result showed that the optimum formula : typha juice 15%, sucrose 4%, citric acid 0.09%, gelatin 0.10%. The mixtures passed 20MPa homogenous pressure, 90 - 100 ℃ sterilization for 10 min, ultimately beverage with white, non-precipitation, the best taste, rich aroma of the typha was obtained. The beverage passed sensory evaluation and physicochemical and microbiological detections. Storage period was up to 6 months. [ Conclusion] Typha lati folia L. Juice Beverage was non-precipitation, the best taste, and rich in nutrition.

  6. Characterization and cloning of a stearoyl/oleoyl specific fatty acyl-acyl carrier protein thioesterase from the seeds of Madhuca longifolia (latifolia).

    Science.gov (United States)

    Ghosh, Santosh K; Bhattacharjee, Ashish; Jha, Jyoti K; Mondal, Ashis K; Maiti, Mrinal K; Basu, Asitava; Ghosh, Dolly; Ghosh, Sudhamoy; Sen, Soumitra K

    2007-12-01

    Deposition of oleate, stearate and palmitate at the later stages of seed development in Mahua (Madhuca longifolia (latifolia)), a tropical non-conventional oil seed plant, has been found to be the characteristic feature of the regulatory mechanism that produces the saturated fatty acid rich Mahua seed fat (commonly known as Mowrah fat). Although, the content of palmitate has been observed to be higher than that of stearate at the initial stages of seed development, it goes down when the stearate and oleate contents consistently rise till maturity. The present study was undertaken in order to identify the kind of acyl-ACP thioesterase(s) that drives the characteristic composition of signature fatty acids (oleate 37%, palmitate 25%, stearate 23%, linoleate 12.5%) in its seed oil at maturity. The relative Fat activities in the crude protein extracts of the matured seeds towards three thioester substrates (oleoyl-, stearoyl- and palmitoyl-ACP) have been found to be present in the following respective ratio 100:31:8. Upon further purification of the crude extract, the search revealed the presence of two partially purified thioesterases: a long-chain oleoyl preferring house-keeping LC-Fat and a novel stearoyl-oleoyl preferring SO-Fat. The characteristic accumulation of oleate and linoleate in the M. latifolia seed fat is believed to be primarily due to the thioesterase activity of the LC-Fat or MlFatA. On the other hand, the SO-Fat showed almost equal substrate specificity towards stearoyl- and oleoyl-ACP, when its activity towards palmitoyl-ACP compared to stearoyl-ACP was only about 12%. An RT-PCR based technique for cloning of a DNA fragment from the mRNA pool of the developing seed followed by nucleotide sequencing resulted in the identification of a FatB type of thioesterase gene (MlFatB). This gene was found to exist as a single copy in the mother plant genome. Ectopic expression of this MlFatB gene product in E. coli strain fadD88 further proved that it induced a

  7. Characterization and cloning of a stearoyl/oleoyl specific fatty acyl-acyl carrier protein thioesterase from the seeds of Madhuca longifolia (latifolia).

    Science.gov (United States)

    Ghosh, Santosh K; Bhattacharjee, Ashish; Jha, Jyoti K; Mondal, Ashis K; Maiti, Mrinal K; Basu, Asitava; Ghosh, Dolly; Ghosh, Sudhamoy; Sen, Soumitra K

    2007-12-01

    Deposition of oleate, stearate and palmitate at the later stages of seed development in Mahua (Madhuca longifolia (latifolia)), a tropical non-conventional oil seed plant, has been found to be the characteristic feature of the regulatory mechanism that produces the saturated fatty acid rich Mahua seed fat (commonly known as Mowrah fat). Although, the content of palmitate has been observed to be higher than that of stearate at the initial stages of seed development, it goes down when the stearate and oleate contents consistently rise till maturity. The present study was undertaken in order to identify the kind of acyl-ACP thioesterase(s) that drives the characteristic composition of signature fatty acids (oleate 37%, palmitate 25%, stearate 23%, linoleate 12.5%) in its seed oil at maturity. The relative Fat activities in the crude protein extracts of the matured seeds towards three thioester substrates (oleoyl-, stearoyl- and palmitoyl-ACP) have been found to be present in the following respective ratio 100:31:8. Upon further purification of the crude extract, the search revealed the presence of two partially purified thioesterases: a long-chain oleoyl preferring house-keeping LC-Fat and a novel stearoyl-oleoyl preferring SO-Fat. The characteristic accumulation of oleate and linoleate in the M. latifolia seed fat is believed to be primarily due to the thioesterase activity of the LC-Fat or MlFatA. On the other hand, the SO-Fat showed almost equal substrate specificity towards stearoyl- and oleoyl-ACP, when its activity towards palmitoyl-ACP compared to stearoyl-ACP was only about 12%. An RT-PCR based technique for cloning of a DNA fragment from the mRNA pool of the developing seed followed by nucleotide sequencing resulted in the identification of a FatB type of thioesterase gene (MlFatB). This gene was found to exist as a single copy in the mother plant genome. Ectopic expression of this MlFatB gene product in E. coli strain fadD88 further proved that it induced a

  8. Gender-based violence, stalking and fear of crime: i principali risultati di una ricerca condotta su un campione di studentesse dell’Università di Perugia / Gender-based violence, stalking and fear of crime : les principaux résultats d’une recherche menée à partir d’un échantillon d’étudiantes universitaires de l’Université de Pérouse (Italie) / Gender-based violence, stalking and fear of crime: The main results of a research among female students of the University of Perugia

    OpenAIRE

    Barro Martina

    2013-01-01

    L’articolo presenta i principali risultati di una ricerca empirica condotta su un campione di studentesse universitarie dell’Università di Perugia. Il questionario verteva su episodi di molestie sessuale, di stalking e di atti sessuali non voluti che potevano essere capitati alle studentesse tanto nell’ambiente universitario che in generale. Oltre ai comportamenti citati, sono stati analizzati i dati sul contesto in cui sono avvenuti i fatti di molestia, sulle reazioni, sulle conseguenze e su...

  9. 蒲菜蔬菜纸的制备%Preliminary Study on Processing Technologies of Vegetable Paper by using Typha Latifolia

    Institute of Scientific and Technical Information of China (English)

    万国福; 张兰

    2012-01-01

    This paper studied the color protection and tackiness agent of vegetable paper by using Typha latifolia. According to the single-factor experiments and orthogonal experiments, the best processing conditions were as follows: Typha latifolia to water ratio (g/mL) 1:1, the time of color protection 1 min, Na2SO3 0.15% and the time of color-protection 1 min. The best fomular of tackiness agent contained 0.2% CMC-Na, 0.8% sodium alginate and 3.0% glycerol. Under such conditions, the product showed good paper properties, tackiness, stripping and brittleness.%本文以蒲菜为原料制作蔬菜纸,通过对蒲菜纸制作过程中的护色和粘结剂选择进行单因素及正交试验分析确定最佳工艺参数.结果表明,护色条件为料水比( g/mL) 1∶2,漂烫时间1min,护色剂亚硫酸钠浓度0.15%;最佳粘结剂配方为0.2% CMC-Na、0.8%海藻酸钠、3.0%甘油.按此条件生产出的蒲菜纸色泽鲜艳、成纸性、胶黏性、易揭性、脆性好.

  10. 大叶冬青不同混交造林模式的生长效果比较%Comparison of growth of Ilex latifolia Thunb among different mixed afforestation models

    Institute of Scientific and Technical Information of China (English)

    李金宝

    2012-01-01

    Mixed afforestation experiment of Ilex latifolia Thunb was conducted by randomized complete block design.The results showed that I.latifolia Thunb and Pinus massoniana Lamb were adaptable for the climate and soil of the site,and P.massoniana Lamb was an ideal species to mix with I.latifolia Thunb.The mixed planting of I.latifolia Thunb × P.massoniana Lamb was able to promote the growth of them and should be popularized in the plantation under the same conditions.When Fokienia hodginsii(Dunn) Henry et Tho or Manglietia yuyuanensis Law was mixed with I.latofolia Thunb.the growth of trees was also promoted,so the mixed forest of I.latifolia Thunb and F.hodginsii(Dunn) Henry et Tho or M.yuyuanensis Law could be created according to particular conditions.Betula luminifera was not adaptable for the conditions of the experimental site,so the mixed plantation of I.latifolia Thunb and B.luminifera proved to be a failure in growing well.%采用完全随机区组进行大叶冬青混交试验。结果表明,大叶冬青、马尾松比较适合试验地气候、土壤,马尾松是大叶冬青的较佳混交树种,大叶冬青×马尾松混交能够促进大叶冬青和马尾松的生长,可在同立地条件林地进行推广;建柏、乳源木莲与大叶冬青混交也能不同程度地促进林木的生长,可根据具体情况营造大叶冬青和建柏或乳源木莲的混交林;光皮桦不能适应该立地条件,大叶冬青和光皮桦混交林的生长状况不良。

  11. Spectrometric Determination of Metal Elements in Typha latifolia L.from Jianshui of Yunnan%云南建水草牙中金属元素的光谱测定

    Institute of Scientific and Technical Information of China (English)

    郭俊明; 戴建辉; 白红丽; 张虹; 王宝森

    2011-01-01

    [目的]测定云南建水草牙(Typha lotifolia L.)中8种金属元素的含量,以期为这一资源的开发利用提供科学依据.[方法]采用火焰原子吸收光谱法测定草芽中的Fe、Zn、Cu、Ni、Co、Cd、Pb和Cr元素的含量.[结果]建水草牙分有多种时人体有益的矿物元素,7种金属元素含量顺序为Fe>Zn >Cu>Ni>C0>Cd >Pb,Cr未检测出;Fe元素含量较高,而Cu含量相对较低.[结论]建水草牙含矿物元素较丰富.%[Objective ] To determine the content of 8 metal elements in Typha latifolia L. from Jianshui County of Yunnan Province, so at to provide a scientific basis for development and utilization of this resources. [Method] Contents of 8 metal elements including Fe, Zn, Cu, Ni, Co, Cd, Pb and Cr were determined by flame atomic absorption spectrometry. [Result] Typha latifolia L. was rich in various mineral elements to human health. The content ordet of 7 analyzed mineral elements was Fe>Zn>Cu>Ni >Co >Cd>Pb. Cr element could not be detected in tested sample. The content( Fe was higher in Typha latifolia L. , while Cu was relatively lower. [ Conclusion ] There were abundant mineral el-ements which were necessary fur people in the Typha latifolia L..

  12. Mesorhizobial strains nodulating Anagyris latifolia and Lotus berthelotii in Tamadaya ravine (Tenerife, Canary Islands) are two symbiovars of the same species, Mesorhizobium tamadayense sp. nov.

    Science.gov (United States)

    Ramírez-Bahena, Martha Helena; Hernández, Mariano; Peix, Alvaro; Velázquez, Encarna; León-Barrios, Milagros

    2012-07-01

    Barranco de Tamadaya is a deep ravine located in southern Tenerife, which is included within a protected area where several endemic plants grow. Among them, two legumes are catalogued as critically endangered, Anagyris latifolia and Lotus berthelotii. Rhizobial strains isolated from their root nodules grown in soil samples from this ravine harboured symbiotic genes belonging to two distant symbiovars, but they shared identical 16S rRNA gene sequences (rrs). The phylogeny based on the rrs sequences placed these isolates in a separate subbranch that did not include any of the currently recognised Mesorhizobium species, but the resolution of the ribosomal tree did not permit further taxonomic conclusions. Nevertheless, multilocus sequence analysis (MLSA) of four housekeeping genes (atpD, recA, glnII and dnaK) and the rrs gene generated a highly supported Bayesian phylogeny, identifying these isolates as a new Mesorhizobium lineage. DNA-DNA hybridisation homology percentages were lower than 30% compared to type strains of the closest related species, and supported the phylogenetic data. Phenotypic characterisation also distinguished this lineage from the other closest Mesorhizobium species. The polyphasic approach thus confirmed that the isolates represented a novel species for which we propose the name Mesorhizobium tamadayense sp. nov. The type strain is Ala-3(T) (CECT 8040(T), LMG 26736(T)).

  13. Comparative study of bio-ethanol production from mahula (Madhuca latifolia L.) flowers by Saccharomyces cerevisiae cells immobilized in agar agar and Ca-alginate matrices

    Energy Technology Data Exchange (ETDEWEB)

    Behera, Shuvashish; Mohanty, Rama Chandra [Department of Botany, Utkal University, Vani Vihar, Bhubaneswar 751004, Orissa (India); Kar, Shaktimay; Ray, Ramesh Chandra [Microbiology Laboratory, Central Tuber Crops Research Institute (Regional Centre), Bhubaneswar 751019, Orissa (India)

    2010-01-15

    Batch fermentation of mahula (Madhuca latifolia L., a tree commonly found in tropical rain forest) flowers was carried out using immobilized cells (in agar agar and calcium alginate) and free cells of Saccharomyces cerevisiae. The ethanol yields were 151.2, 154.5 and 149.1 g kg{sup -1} flowers using immobilized (in agar agar and calcium alginate) and free cells, respectively. Cell entrapment in calcium alginate was found to be marginally superior to those in agar agar (2.2% more) as well as over free cell (3.5% more) as regard to ethanol yield from mahula flowers is concerned. Further, the immobilized cells were physiologically active at least for three cycles [150.6, 148.5 and 146.5 g kg{sup -1} (agar agar) and 152.8, 151.5 and 149.5 g kg{sup -1} flowers (calcium alginate) for first, second and third cycle, respectively] of ethanol fermentation without apparently lowering the productivity. Mahula flowers, a renewable, non-food-grade cheap carbohydrate substrate from non-agricultural environment such as forest can serve as an alternative to food grade sugar/starchy crops such as maize, sugarcane for bio-ethanol production. (author)

  14. Identification of Abiotic and Biotic Factors Causing Deterioration During Storage and Development of Storage Techniques for Mahua (Madhuca indica Syn. Bassia latifolia flowers

    Directory of Open Access Journals (Sweden)

    Basanta Kumar Das

    2010-09-01

    Full Text Available Mahua (Madhuca indica syn. Bassia latifolia flowers, occupy an important position in the life of the tribal in many parts of India. Particularly, the flowers of the plant are sugar rich and in certain cases it is the only source of livelihood for those people. However, its nutrient quality deteriorates during the postharvest storage and thus, poses a serious problem of adequate storage. In order to determine the cause of spoilage and to develop the measures to check it, collected flowers were stored using two methods in this investigation; first, under normally practiced conditions (NPS, i.e., the practice adopted by the flower’s collectors, and second, oven dried, powdered with liquid nitrogen, and stored at 00C (±10C in different small airtight sterilized vials labelled as laboratory processed samples (LPS. Both LPS and NPS were stored for a year i.e. from one harvesting season to the next. Experiments were carried out, at every month interval, to identify the factors responsible for spoilage of flowers during storage. LPS did not exhibit deterioration in the nutrient value throughout the year of storage, but NPS showed spoilage due to various biotic and abiotic factors comprised of moisture, temperature and microorganisms. To check postharvest spoilage various innovative storage techniques like physical and chemical treatments were experimented. Results revealed that storage at low temperature by liquid nitrogen treatment and chemical conversion to oxalic acid were the most effective techniques for a long-term storage.

  15. CRECIMIENTO Y DESARROLLO DE LA LIMA ÁCIDA (Citrus latifolia Tanaka, cv. Tahití, EN SUELOS CON LIMITACIONES POR PROFUNDIDAD EFECTIVA, EN UN BOSQUE SECO TROPICAL GROWTH AND DEVELOPMENT OF LIME (Citrus latifolia Tanaka, cv. Tahiti, IN SOILS WITH LIMITATIONS BY EFFECTIVE DEPTH IN A TROPICAL DRY FOREST

    Directory of Open Access Journals (Sweden)

    Gabriel Fernando Ochoa Agudelo

    2012-06-01

    Full Text Available Resumen. Se evaluó la evolución de la biomasa, la distribución diferencial de ésta entre los órganos y algunos parámetros del crecimiento y desarrollo, de la lima ácida (Citrus latifolia Tanaka, cv. Tahití, injertada sobre Carrizo y Kryder. El cultivo se estableció en suelos con limitaciones por profundidad efectiva (PE entre 10 y 20 cm; las evaluaciones se realizaron a los 24, 32 y 39 meses después del trasplante. Se debe tener presente que el crecimiento de las raíces está condicionado a los atributos edáficos en donde se establecen los cultivos, en especial los perennes, en cuyo caso existen requerimientos mínimos de PE para el adecuado desarrollo de los árboles; se establecieron diferencias entre la biomasa acumulada, como consecuencia de ésta limitación. Al comparar el crecimiento de las plantas con la PE se evidenció que los individuos que crecieron en suelos con PE de 20 cm, presentaron un desarrollo relativamente semejante a plantaciones sin limitaciones de este tipo; mientras que, en suelos con PE inferior a 10 cm, la respuesta fue 3 a 5 veces menor.Abstract. It was evaluated the evolution of biomass, its differential distribution among the organs and some parameters of growth and development of acid lime (Citrus latifolia Tanaka, cv. Tahiti, grafted on Carrizo and Kryder patterns. The plants were established in soils with limitations by its effective depth (ED, which ranged between 10 and 20 cm. Assessments were effectuated at 24, 32 and 39 months after transplantation. It is must to keep in mind that root growth is conditioned to the soil attributes of the site where the crops are established, especially in the case of perennials, which have requirements of minimum PE in order to achieve an adequate development. Differences were established between biomass accumulated as a result of the limitation in effective depth. When comparing the growth of plants with ED, we found that the individuals that grew up in soils with

  16. Effect of Cadmium and Lead Stress on the DNA Cross-linking in Zizania latifolia%重金属镉、铅胁迫对茭白DNA交联的影响

    Institute of Scientific and Technical Information of China (English)

    黄凯丰; 江解增

    2009-01-01

    To study the contents of dietary fiber (DF) and crude fiber in gall's of Zizania latifolia grown in stress with Cd2+ I00 nag/L, Pb2+ 1 000 mg/L and their combined stress, a single-harvested cultivar Jiangshujiao and a double-harvested cultivar Fenghongzao of Zizania latifolia were cultured in oil with organic fertilizer (OF) and inorganic fertilizer (IF). The results showed that, Cd2+ and Pb2+ stress could reduce the amount of extracted DNA and the hyperehromicity of DNA in the leaves of Zizania latifolia remarkably. Treated with combined stress of Cd2+ and Pb2+, the amount of extracted DNA in the leaves of Zizania latifolia was lower than single stress. The hyperchromicity of DNA had a larger descendent range when treated with combined stress than single stress. Treated with inorganic fertilizer, the amount of extracted DNA in the leaves of Zizania latifolia was lower than treated with organic fertilizer. The hyperchrornicity of DNA had a larger descen-dent range when treated with inorganic fertilizer than organic fertilizer. The amount of extracted DNA and the hyper-chromicity of DNA in leaves of Jiangshu were higher than that of Fenghongzao at the same treatment.%以单季茭白品种蒋墅芟和双季茭白品种葑红早为试材,对Cd2+、pb2+的单一及其复合胁迫,进行有机肥和无机肥处理,测定了两茭白品种叶片中DNA的提取量以及DNA增色效应的变化.试验结果表明.Cd2+、Pb2+胁迫均能显著降低两茭白品种叶片中DNA提取的量及DNA增色效应的程度.不同胁迫处理方式间以复合胁迫处理下茭白叶片中DNA提取的量显著低于单一胁迫处理,复合胁迫处理时茭白叶片DNA的增色效应下降的幅度显著大于单一胁迫处理.不同肥料处理间则以无机肥处理时DNA提取的量显著低于有机肥处理;而无机肥处理时茭白叶片DNA增色效应下降的幅度显著大于有机肥处理.品种间同比均以蒋墅茭较高.

  17. Relationship of seed traits on initial progeny growth performance and divergence studies in Madhuca latifolia Macb. for further use in tree improvement

    Directory of Open Access Journals (Sweden)

    BN Divakara

    2014-12-01

    Full Text Available Evaluation of twenty-three genotypes of M. latifolia was carried out based on relationship of seed traits with initial progeny growth performance and divergence studies as a scope for further breeding programme. Variability studies revealed that, more than twelve accessions recorded above average for 100-seed weight (247.5±49.2, oil content (43.8±3.7 and volume index (346.0±97.7. The maximum values observed in studied CPTs were as follows: seed length (39.1 mm in CPT-15 genotype, seed breadth (19.2 mm in CPT–8 and CPT–9, aspect ratio (2.2 in CPT-6 and CPT-15, 2D surface area (501.4 and 491.6 mm2 in CPT-9 and CPT-3 respectively. CPT–16 recorded maximum for 100 seed weight (282.4 g and oil content (51.2%. However, maximum volume index was recorded by CPT–3 (578.3 cm3 followed by CPT–16 (496.0 cm3. The phenotypic and genotypic coefficients of variations are close to each other for all traits, except volume index that exhibited striking difference between PCV (40.0% and GCV (19.9% indicating that for most traits genetic control was quite high. Trait oil content and 100 seed weight expressed high heritability (93.5%, 93.0% accompanied with moderate genetic advance (17.2%, 15.6%, indicating that, heritability is due to additive gene effects and selection may be effective. At genotypic level 100 seed weight registered positive significant correlation with height (0.73 and seed breadth, oil content with volume index (0.55, 0.71. Hence seeds with large breadth, high seed weight and oil content may be selected for producing better progenies. Since traits viz. 100 seed weight and oil content are under strong genetic control, improvement in these characters can bring improvement in volume index. On the basis of the divergence, the 23 genotypes studied were grouped into 5 clusters, indicating wide diversity. The clustering pattern shows that geographical diversity is not necessarily related to genetic diversity. The genotypes in cluster IV and V

  18. 茭白总蛋白质双向电泳技术体系的建立%Establishment of Two-Dimensional Electrophoresis Conditions for Proteomic Analysis of Zizania latifolia

    Institute of Scientific and Technical Information of China (English)

    罗海波; 陈伟; 周静峰; 宋慧波; 郁志芳

    2013-01-01

    In order to establish the two-dimensional electrophoresis (2-DE) conditions of Zizania latifolia,the effects of different protein extraction methods,loading amount,pH ranges of IPG strip and gel concentration,etc,on 2-DE maps were investigated.The results showed that the modified phenol extraction protocol is more suitable for total protein extraction of Z.latifolia compared with TCA/acetone protocol.And a high quality 2-DE map with more protein spots,clear background and high protein point resolution was obtained using the following optimized procedure:loading 1.0mg protein sample on 17 cm IPG strip with pH 4-7,SDS-PAGE with 12 g/dL gel concentration,and finally detecting proteins with coomassie brilliant blue G250 staining.The research provides a basis for further study of the Z.latifolia proteomics.%为建立茭白总蛋白质双向电泳技术体系,研究了TCA/丙酮沉淀法和改良酚抽法两种不同总蛋白质提取方法、蛋白质上样量、pH值范围及凝胶质量浓度等条件对2-DE的影响.结果表明,改良酚抽法更适合茭白总蛋白质的提取,采用17 cm、pH 4~7的胶条、1.0 mg的蛋白质上样量、12 g/dL的凝胶浓度、考马斯亮蓝G-250胶体考染法染色,最终可获得蛋白质点较多,背景清晰,分辨率较高的2-DE图谱,为进一步开展茭白差异蛋白质组学研究奠定了基础.

  19. Effects of Fungicide Fenaminosulf and Plant Growth Regulator on Gall For-mation of Zizania latifolia%杀菌剂敌磺钠及植物生长调节剂对茭白孕茭的影响

    Institute of Scientific and Technical Information of China (English)

    应荣; 崔海峰; 倪方群; 俞晓平; 叶子弘

    2014-01-01

    以‘龙茭二号’为研究对象,通过在茭白植株不同生长发育阶段分别喷施杀菌剂敌磺钠和植物生长调节剂,比较分析其对茭白结茭的促进效果。对6个不同处理组合及对照材料的株高、内源激素含量变化及结茭情况的统计分析发现:喷施一定浓度的敌磺钠对茭白结茭具有明显的促进作用,以敌磺钠(标准浓度)+营养液和敌磺钠(标准浓度)对茭白结茭产量的提高最为明显。植物生长调节剂处理能明显促进茭白早期植株生长,但对最终株高影响不大。采用间接酶联免疫法对茭白茎部3种激素(IAA、ABA和GA3)含量进行的比较分析表明:敌磺钠处理能够明显降低ABA含量,但对IAA和GA3含量没有明显影响;茭白结茭前ABA含量的降低可能有助于茭白结茭。%In this research, the promotion of fungicide fenaminosulf and plant growth regulator in the gall for-mation had been produced by spraying fenaminosulf and plant growth regulator combinations at different growth development phases of Zizania latifolia‘Longjiao 2’. After comparative and statistical analyzing of the plant heights, changes of endogenous hormone (IAA, ABA and GA3) in stem and yields of gall formation, it was found that the spraying of fenaminosulf with a certain concentration could signiifcantly promote the yields of gall formation in Z. latifolia, especially the spraying combinations of fenaminosulf (standard concentration) with nutrient solution and fenaminosulf (standard concentration). The promotions of plant growth regulator were also found, which was related to the different contents of plant growth regulator. Besides, the treatments of plant growth regulator could signiifcantly promote the early growth of plants, but little effect on the ifnal plants height. Using the methods of indirect ELISA, the contents of IAA, ABA and GA3 at the residual part of stem in Z. latifolia were compared and analysed. It was showed

  20. 纤维素酶—乙醇法提取苦丁茶中总黄酮%Extraction of Total Flavonoids from the Leaves of Ilex latifolia by Cellulase-Ethanol Method

    Institute of Scientific and Technical Information of China (English)

    黄靖

    2012-01-01

    Total flavonoids were extracted from leaves of Ilex latifolia (Kuding tea) by cellulase-ethanol method. The effects of cellulose dose, enzymolysis time, temperature, pH, volume fraction of ethanol, and dose of ethanol on yield of total flavonoids were studied by single factor tests so as to optimize the extraction conditions. The results showed that the optimum extraction conditions were obtained as follows, dose of cellulose enzyme, 1.0 mg per 5.0 g Kuding tea flour; enzymolysis time, 2.0 h; enzymolysis temperature, 55℃; pH, 4.5; volume fraction of ethanol, 60%; dose of ethanol, 35 mL per 5.0 g Kuding tea flour. The yield of flavonoids was up to 7.80% under these conditions.%采用纤维素酶酶解预处理与乙醇浸提法相结合从苦丁茶中提取总黄酮.通过单因素试验考察酶用量、酶解时间、酶解温度、pH、乙醇体积分数和乙醇用量对总黄酮提取率的影响,优化提取工艺条件.结果表明,优化的提取工艺条件为0.5 g苦丁茶粉末中加入纤维素酶1.0mg、酶解时间2.0 h、酶解温度55℃、酶解pH 4.5、体积分数60%的乙醇用量35 mL,最佳提取工艺条件下苦丁茶中总黄酮提取率达7.80%.

  1. 大叶冬青苦丁茶多糖提取、纯化与抗氧化活性研究%Extraction,Purification and Antioxidant Activity in Vitro of Polysaccharides from Kudingcha Made from Ilex latifolia Thunb

    Institute of Scientific and Technical Information of China (English)

    赵天湖; 范嘉龙; 闫冬; 徐人杰; 郎昌野; 孙怡; 曾晓雄

    2011-01-01

    Kudingcha made from Ilex latifolia Thunb was defatted with 85% ethanol, and then extracted with hot water,decolored by S-8 macroporous resin, precipitated by ethanol, and dried to afford crude polysaccharide (ILPS). Four purified polysaccharides, ILPS-1, ILPS-2, ILPS-3 and ILPS-4, were gained from crude ILPS by DEAE-52 anion-exchange chromatography. Furthermore. the antioxidant activities in vitro of the crude ILPS and its purified fractions were evaluated by determinations of scavenging activities on 1. 1-diphenyl-2-picrylhydrazyl (DPPH) free radicals, superoxide anion radicals (O2· - ), hydroxyl radicals (·OH) , ferric reducing-antioxidant power (FRAP) and chelating capacity to Fe2+. The results indicated that ILPS exhibited strong antioxidant activity, and the antioxidant activity was significantly correlated with its concentration.%大叶冬青苦丁茶经85%乙醇溶液脱脂、热水提取、S-8大孔树脂脱色、醇沉、干燥,得到大叶冬青苦丁茶粗多糖.粗ILPS经DEAE-52纤维素阴离子交换层析柱分离,得到4个多糖组分ILPS-1,ILPS-2,ILPS-3和ILPS-4.采用化学法分别测定了粗多糖及其纯化组分的体外清除自由基(DPPH·自由基,O2-·自由基,·OH自由基)能力、还原能力以及螯合金属离子能力.结果表明,大叶冬青苦丁茶多糖具有较强的体外抗氧化活性,并且抗氧化能力与多糖浓度之间存在良好的相关性.

  2. Optimum on acid-extraction technoloGy of pectin in Stauntonia latifolia pericarp by response surface methodoloGy%响应面法优化八月瓜果皮中果胶的酸提取工艺

    Institute of Scientific and Technical Information of China (English)

    李雪峰; 符智荣; 欧阳玉祝; 黄亚娟

    2014-01-01

    用盐酸提取八月瓜果皮的果胶,考察了pH 值、提取时间和提取温度对果胶得率的影响。结果表明,pH值、温度对果胶得率有显著影响,八月瓜果皮果胶提取的最佳工艺条件为:pH=1,提取时间156 min,提取温度91℃,果胶得率为7.11%。%With StauNtONia latifOlia as experimental raw material,extraction technology of pectin in StauN-tONia latifOlia pericarp was studied. Influence of pH,extraction time and extraction temperature on extrac-tion ratio of pectin were investigated by response surface methodology. Experimental results show that the best process condition of extraction ratio of pectin is 7. 11% at pH=1 and 91 ℃,extraction 156 min.

  3. Gender-based violence, stalking and fear of crime: i principali risultati di una ricerca condotta su un campione di studentesse dell’Università di Perugia / Gender-based violence, stalking and fear of crime : les principaux résultats d’une recherche menée à partir d’un échantillon d’étudiantes universitaires de l’Université de Pérouse (Italie / Gender-based violence, stalking and fear of crime: The main results of a research among female students of the University of Perugia

    Directory of Open Access Journals (Sweden)

    Barro Martina

    2013-07-01

    Full Text Available L’articolo presenta i principali risultati di una ricerca empirica condotta su un campione di studentesse universitarie dell’Università di Perugia. Il questionario verteva su episodi di molestie sessuale, di stalking e di atti sessuali non voluti che potevano essere capitati alle studentesse tanto nell’ambiente universitario che in generale. Oltre ai comportamenti citati, sono stati analizzati i dati sul contesto in cui sono avvenuti i fatti di molestia, sulle reazioni, sulle conseguenze e sul senso di minaccia percepito. Nelle conclusioni l’autrice propone un’interpretazione della rappresentazione sociale della molestia sessuale basata sulla teoria dell’identità sociale e su quella dell’autocategorizzazione del sé.RésuméCet article rend compte des principaux résultats d'une enquête menée à partir d’un échantillon d'étudiantes universitaires de l'Université de Pérouse (Italie. Le questionnaire avait pour objet les épisodes de harcèlement sexuel, de traque furtive (stalking et de violence sexuelle qui pouvaient avoir eu lieu dans le milieu universitaire comme à l’extérieur. En outre, l’auteur analyse le contexte, les réactions, les conséquences et le sentiment de menace perçu par les étudiantes. En conclusion, l’auteur propose une interprétation de la représentation sociale du harcèlement sexuel s’appuyant sur les théories de l'identité sociale et de l'auto-catégorisation.AbstractThis article presents the main results of an empirical research among female students of the University of Perugia (Italy. The questionnaire dealt with sexual harassment, stalking and unwanted sexual acts experienced by the girls inside and outside the university. In addition, contextual data connected to these incidents, the reaction, the consequences and the perceived threat are analysed. Finally, the author proposes an explanation for a social representation of sexual harassment based on Social Identity and Self

  4. Grande impresa e decisioni pubbliche. La Fiat da Campione nazionale atipico a global player

    OpenAIRE

    Germano Luca

    2007-01-01

    This paper starts from a mainframe inspired on C. Lindblom's theory (1977): big business and public decisions-makers had always had a privileged relationship. However, as an important variation from Lindblom's affirmations, the intervention of the State in Italy has not been addressed to satisfied all the requests of big enterprises. Its intervention has been characterised as a negotiation process orientated by the reciprocal advantages of both involved actors. We present a case study, the Fi...

  5. Infection of the Anther-smut Microbotryum violaceum in Populations of Silene dioica: Variation in Floral Morphology, Patterns of Spore Deposition and Pathogen-Pollinator Mediated Selection%麦瓶草种群中的花药黑粉菌感染:花的形态学变异、菌孢子散布式样及病原菌-传媒调节性选择

    Institute of Scientific and Technical Information of China (English)

    刘登义; Ulla CARLSSON

    2002-01-01

    The anther-smut Microbotryum violaceum (Brandenburger and Schvinn) G. Deml. And Oberw.) causes a systemic infection of its host Silene dioica (L.) Clairv., resulting in sterility and production of teliospores (dispersal propagules) in flowers. These spores are transmitted to healthy plants mainly by flower visitors. The behavioral responses of flower visitors to a variation in floral characters, are not only likely to affect rates of pollen export/import, but also the rate of spore deposition and probability of disease. In a transplantation experiment, using plants from four different populations, we tested for correlation between variation in female floral morphology and patterns of spore and pollen deposition, and a resulting risk of disease. The source populations in this experiment were located on four islands in Skeppsvik archipelago in northern Sweden, and represented a gradient of disease incidence from completely healthy (Island 1), low incidence (Island 2) to high incidences (Islands 3 and 4) of disease. Fifty plants from each population were transplanted to the center of the population on Island 4. There were significant differences among the transplants in floral characters, I.e. Corolla size, style length and ovule number. Plants from the non-diseased population had larger flowers and longer styles than plants from the highly diseased populations. Numbers of pollen grains and spores deposited on flowers were strongly and positively correlated. We found that plants originating from the non-diseased population captured approximately 4 times more pollen and 9 times more spores per flower than the individuals from the resident population (Island 4, population 4). The incidences of disease among plants from the four populations differed significantly, and was 37%, 20%, 18% and 0 for populations 1, 2, 3 and 4 respectively. In a survey of ten populations we found a significant negative correlation between the mean style length (positively

  6. On Species Diversity,Niche Breath and Interspecies Association in Communities Invaded by Spermacoce latifolia Zhejiang Province%浙江省阔叶丰花草入侵群落物种多样性、生态位及种间联结研究

    Institute of Scientific and Technical Information of China (English)

    洪思思; 缪崇崇; 方本基; 胡仁勇; 丁炳扬

    2008-01-01

    基于对浙江省10个代表性样地的调查,应用物种丰富度指数(S)、Simpson指数(Ds)、Shannon-Wiener 指数(H')和Pielou均匀度指数(J),分析了阔叶丰花草(Spermacoce latifolia)入侵群落的物种多样性;并通过测定群落中20个种的生态位宽度、生态位重叠、x2测验、联结系数(Ac)和共同出现百分率(PC),从多个方面探讨了阔叶丰花草在群落中对环境资源的利用状况、与其他物种的相互关系和相对地位.结果表明:(1)阔叶丰花草的入侵降低了群落的物种多样性;(2)群落中阔叶丰花草生态位宽度最大,说明阔叶丰花草对环境的适应能力较强,很容易与其他物种发生竞争;(3)阔叶丰花草与升马唐(Digitaria ciliaris)的生态位重叠值最大,野塘蒿(Conyza bonariensis)、莎草(Cyperus rotundus)次之;(4)阔叶丰花草入侵群落中所有种间没有呈现出显著的相关性,阔叶丰花草只与升马唐、野塘蒿有一定的正联结,与莎草、鸭跖草(Commelina communis)、母草(Lindernia crustacea)等有一定的负联结.

  7. Growth performance of 12 year old air layered Madhuca latifolia

    Directory of Open Access Journals (Sweden)

    Sangram Bhanudas Chavan

    2014-12-01

    It is concluded that, the growth of air-layered Mahua is better when compared to published literature on seedling originated mahus. Based on this study, it is proved that air layering of mahua is cheap and best method to produce quality planting material. Further large scale investigation on performance of air-layered mahua plantations will provide early returns in terms of flowers and seed to sustain local livelihood. The stature of air layered mahua is well suited for agroforestry plantations.

  8. Hypolipidemic activity of gum ghatti of Anogeissus latifolia

    Directory of Open Access Journals (Sweden)

    K.M.M Parvathi

    2009-01-01

    Full Text Available Cardiovascular diseases are becoming an increasing problem worldwide and hypercholesterolemia has been correlated for coronary heart diseases. Currently available hypolipidemic drugs have been associated with number of side effects. Herbal treatment for hyperlipidemia poses no side effects and is relatively cheap and locally available. In view of this, the present study was carried out to investigate the effect of gum ghatti of Anogeissus lalifolia on serum lipid levels of albino rats. Rats were made hyperlipidemic by the oral administration of cholesterol (400mg/kg body weight/day along with cholic acid (50mg/kg in coconut oil. The hypolipidemic effect was compared with control. The rats were divided into six groups of six animals each. In atherogenic diet induced hyperlipidemic model, the rats receiving treatment with gum ghatti at 250 mg/kg dosage showed significant reduction in serum triglyceride (82.75±0.63 only and there was no significant changes either in serum total cholesterol or elevation in HDL. Whereas, at 500 and 750 mg/kg dosage showed significant reduction in serum total cholesterol (72.85±0.60, 68.17±0.95 and serum triglyceride (78.92±0.34, 75.93±1.05. Further, the 750 mg/kg dose has also exhibited significant elevation in high density lipoprotein cholesterol (41.13±0.37.

  9. Rice field agroecosystem investigation : environmental and toxicological assessment; Indagine su una risaia campione: analisi ambientali e chimico-tossicologiche

    Energy Technology Data Exchange (ETDEWEB)

    Bari, A.; Minciardi, M.; Rossi, G. [ENEA, Centro Ricerche Saluggia, Vercelli (Italy). Dip. Ambiente; Bonotto, F.; Paonessa, F.; Troiani, F. [ENEA, Centro Ricerche Saluggia, Vercelli (Italy). Dip. Energia; Rosa, S. [ENEA, Centro Ricrche Casaccia, Rome (Italy). Dip. Ambiente; Cormegna, M. [Ente Nazionale Risi, Centro Ricerche sul Riso, Castello d`Agogna, Pavia (Italy)

    1995-10-01

    The rice-field agroecosystem, even if deeply anthropically determined, can be considered substitute of the plain wet lands, now almost all disappeared in the part of the territory has been considering. The aim of the research we started was the analysis and the ecological characterization of this environment and the assessment of the effects of the different agronomical practices, relating to the conservation of the biodiversity in a plain wetland. The ENEA Environmental Biology and Nature Conservation Division of Saluggia (VC) and Casaccia (Roma), in co-operation with ENEA ERG-RAD-LAB Division of Saluggia and the Rice Research Center of Castello d`Agogna (PV) associated to Rice National Society, started a preliminary research on a sample rice field, aiming to evaluate, using different methodologies, the destiny of the chemical substances (herbicides, fungicidals, heavy metals and other chemical compounds) introduced through cultivation practices or arrived by irrigation systems.

  10. Environ: E00600 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available E00600 Arenaria juncea, Silene fortunei, Silene jenisensis, Gypsophila oldhamiana r...oot Crude drug Arenaria juncea, Arenaria [TAX:3577], Silene fortunei [TAX:411984], Silene jenisensis, Silene...[TAX:3573], Gypsophila oldhamiana, Gypsophila [TAX:146094] Caryophyllaceae (pink family) Arenaria juncea, Silene fortune...[BR:br08305] Dicot plants: others Caryophyllaceae (pink family) E00600 Arenaria juncea, Silene fortunei, Silene jenisensis, Gypsophila oldhamiana root ...

  11. Genetic variation of inbreeding depression among floral and fitness traits in Silene nutans

    DEFF Research Database (Denmark)

    Thiele, Jan; Hansen, Thomas Møller; Siegismund, Hans Redlef;

    2010-01-01

    decoupled. There was a trend that the smaller population was less affected by ID than the large one, although the differences were not significant for most traits. Hence, evidence for purging of deleterious alleles remains inconclusive in this study. Genetic variation in ID among paternal families...

  12. Gas pressure generation from irradiated fuel during transient heating in the Silene reactor

    International Nuclear Information System (INIS)

    Several in-pile experiments have been performed to measure the fission gases release out of an irradiated fuel during a fast power transient (0,7 - 1,5 kJ/g in 7 ms). They have shown that a fuel subjected to such a transient can release a large amount of fission and other gases, namely H2 and CO. Special caution during the handling, followed by stoving before the transient have suppressed the spurious CO and H2 in the last experiments. We can now determine the kinetics of intrinsic gases release. The fuel can liberate up to half of its fission gases below melting in a frame time included between 120 and 200 ms for 90% releasing. In the case of full melting during the transient, the fission gases release reaches 100% and seems slower

  13. Wild food plants used in the villages of the Lake Vrana Nature Park (northern Dalmatia, Croatia

    Directory of Open Access Journals (Sweden)

    Łukasz Łuczaj

    2013-12-01

    Full Text Available Croatia is a country of diverse plant use traditions, which are still insufficiently documented. The aim of this study was to document local traditions of using wild food plants around Lake Vrana (northern Dalmatia, Zadar region.  We interviewed 43 inhabitants of six traditional villages north of Lake Vrana. On average 12 species were listed, which in total produced an inventory of 55 food plants and 3 fungi taxa. Wild vegetables were most widely collected, particularly by older women who gathered the plants mainly when herding their flocks of sheep. Wild fruits and mushrooms were rarely collected. The former used to be an important supplementary food for children, or for everyone during times of food shortage, and the latter were relatively rare due to the dry climate and shortage of woods. The most commonly collected plants are wild vegetables: Cichorium intybus, Foeniculum vulgare, Sonchus oleraceus, Asparagus acutifolius, Papaver rhoeas, Rumex pulcher, Daucus carota, Allium ampeloprasum and Silene latifolia.

  14. Stigma towards Eating Disorders in Italian Students (STEaDIS): Studio osservazionale delle opinioni sull’Anoressia e la Bulimia Nervosa in un campione di studenti universitari italiani

    OpenAIRE

    Caslini,

    2015-01-01

    Razionale: La richiesta di cure per l’anoressia (AN) e la bulimia nervosa (BN) rimane bassa rispetto alla diffusione dei disturbi nonostante la disponibilità di trattamenti e l’associazione tra una buona prognosi e la precocità delle cure: una delle motivazioni alla base del problema è insita nelle paure generate dalle credenze e dagli atteggiamenti stigmatizzanti verso i disturbi del comportamento alimentare (DCA). Lo stigma per i disturbi psichiatrici è un fenomeno radicato nella società, d...

  15. [Eda Kalmre. The human sausage factory : a study of post-war rumour in Tartu] / Véronique Vincent-Campion

    Index Scriptorium Estoniae

    Vincent-Campion, Véronique

    2015-01-01

    Arvustus: Kalmre, Eda. The human sausage factory : a study of post-war rumour in Tartu. Amsterdam ; New York : Rodopi, 2013. (On the boundary of two worlds : identity, freedom, and moral imagination in the Baltics, 1570-7121 ; 34)

  16. Sex in advertising: dioecy alters the net benefits of attractiveness in Sagittaria latifolia (Alismataceae)

    OpenAIRE

    Vamosi, Jana C; Vamosi, Steven M.; Barrett, Spencer C.H.

    2006-01-01

    The flowers and inflorescences of animal-pollinated dioecious plants are generally small and inconspicuous in comparison with outcrossing cosexual species. The net benefits of an attractive floral display may be different for dioecious compared to cosexual populations because dioecious species experience a more severe reduction in pollen delivery when pollinators forage longer on fewer individuals. Here, we develop a model that predicts the decrease in pollen delivery in dioecious relative to...

  17. Nutritional value of organic acid lime juice (Citrus latifolia T.), cv. Tahiti

    OpenAIRE

    Carolina Netto Rangel; Lucia Maria Jaeger de Carvalho; Renata Borchetta Fernandes Fonseca; Antonio Gomes Soares; Edgar Oliveira de Jesus

    2011-01-01

    Acid lime can be used as fresh fruit or as juice to increase the flavor of drinks. Therefore, it is necessary to analyze organic acid lime nutritional composition in order to evaluate if there are important differences among those conventionally produced. No significant differences in total titrable acidity, pH, ascorbic acid, sucrose, calcium, and zinc were found between the acid lime juice from organic biodynamic crops and conventional crops. However, the organic biodynamic fruits presented...

  18. Noble strain of Sparassis latifolia produces high content of β-glucan

    Directory of Open Access Journals (Sweden)

    Dong Ju Lee

    2015-08-01

    Conclusions: These results indicate that the nucleotide sequences and the amino acid sequences of RPB2 can be used to identify individual Sparassis sp. The Sparassis strain CLM1 may be best for developing a remedy to prevent or treat cancer and other chronic diseases.

  19. Makomotines A to D from Makomotake, Zizania latifolia infected with Ustilago esculenta

    OpenAIRE

    Choi, Jae-Hoon; Suzuki, Tomohiro; Kawaguchi, Takumi; Yamashita, Kimiko; Morita, Akio; Masuda, Kikuko; Yazawa, Kazunaga; Hirai, Hirofumi; Kawagishi, Hirokazu

    2014-01-01

    NOTICE: this is the author’s version of a work that was accepted for publication in Tetrahedron Letters.Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document.Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Tetrahedron Letters, Volume 55, Issue 26, 25 June 2014. DOI 10.1016/j.tetl...

  20. A comparison of pollen-siring ability and life history between males and hermaphrodites of subdioecious Silene acaulis

    DEFF Research Database (Denmark)

    Philipp, Marianne; Jakobsen, Ruth Bruus; Nachman, Gøsta Støger

    2009-01-01

    was performed in which females were hand A pollen-competition experiment was performed in which females were hand pollinated with a mixture of pollen from males and hermaphrodites, all with known isozyme alleles, which allowed determination of who sired each seed. We recorded plant size, flower morphology...

  1. Plant sex chromosomes: molecular structure and function.

    Science.gov (United States)

    Jamilena, M; Mariotti, B; Manzano, S

    2008-01-01

    Recent molecular and genomic studies carried out in a number of model dioecious plant species, including Asparagus officinalis, Carica papaya, Silene latifolia, Rumex acetosa and Marchantia polymorpha, have shed light on the molecular structure of both homomorphic and heteromorphic sex chromosomes, and also on the gene functions they have maintained since their evolution from a pair of autosomes. The molecular structure of sex chromosomes in species from different plant families represents the evolutionary pathway followed by sex chromosomes during their evolution. The degree of Y chromosome degeneration that accompanies the suppression of recombination between the Xs and Ys differs among species. The primitive Ys of A. officinalis and C. papaya have only diverged from their homomorphic Xs in a short male-specific and non-recombining region (MSY), while the heteromorphic Ys of S. latifolia, R. acetosa and M. polymorpha have diverged from their respective Xs. As in the Y chromosomes of mammals and Drosophila, the accumulation of repetitive DNA, including both transposable elements and satellite DNA, has played an important role in the divergence and size enlargement of plant Ys, and consequently in reducing gene density. Nevertheless, the degeneration process in plants does not appear to have reached the Y-linked genes. Although a low gene density has been found in the sequenced Y chromosome of M. polymorpha, most of its genes are essential and are expressed in the vegetative and reproductive organs in both male and females. Similarly, most of the Y-linked genes that have been isolated and characterized up to now in S. latifolia are housekeeping genes that have X-linked homologues, and are therefore expressed in both males and females. Only one of them seems to be degenerate with respect to its homologous region in the X. Sequence analysis of larger regions in the homomorphic X and Y chromosomes of papaya and asparagus, and also in the heteromorphic sex chromosomes

  2. How to be an attractive male: floral dimorphism and attractiveness to pollinators in a dioecious plant

    Directory of Open Access Journals (Sweden)

    Waelti Marc O

    2009-08-01

    Full Text Available Abstract Background Sexual selection theory predicts that males are limited in their reproductive success by access to mates, whereas females are more limited by resources. In animal-pollinated plants, attraction of pollinators and successful pollination is crucial for reproductive success. In dioecious plant species, males should thus be selected to increase their attractiveness to pollinators by investing more than females in floral traits that enhance pollinator visitation. We tested the prediction of higher attractiveness of male flowers in the dioecious, moth-pollinated herb Silene latifolia, by investigating floral signals (floral display and fragrance and conducting behavioral experiments with the pollinator-moth, Hadena bicruris. Results As found in previous studies, male plants produced more but smaller flowers. Male flowers, however, emitted significantly larger amounts of scent than female flowers, especially of the pollinator-attracting compounds. In behavioral tests we showed that naïve pollinator-moths preferred male over female flowers, but this preference was only significant for male moths. Conclusion Our data suggest the evolution of dimorphic floral signals is shaped by sexual selection and pollinator preferences, causing sexual conflict in both plants and pollinators.

  3. Permanent Genetic Resources added to Molecular Ecology Resources Database 1 August 2009-30 September 2009.

    Science.gov (United States)

    Abdoullaye, Doukary; Acevedo, I; Adebayo, Abisola A; Behrmann-Godel, Jasminca; Benjamin, R C; Bock, Dan G; Born, Céline; Brouat, Carine; Caccone, Adalgisa; Cao, Ling-Zhen; Casado-Amezúa, P; Catanéo, J; Correa-Ramirez, M M; Cristescu, Melania E; Dobigny, Gauthier; Egbosimba, Emmanuel E; Etchberger, Lianna K; Fan, Bin; Fields, Peter D; Forcioli, D; Furla, P; Garcia de Leon, F J; García-Jiménez, R; Gauthier, Philippe; Gergs, René; González, Clementina; Granjon, Laurent; Gutiérrez-Rodríguez, Carla; Havill, Nathan P; Helsen, P; Hether, Tyler D; Hoffman, Eric A; Hu, Xiangyang; Ingvarsson, Pär K; Ishizaki, S; Ji, Heyi; Ji, X S; Jimenez, M L; Kapil, R; Karban, R; Keller, Stephen R; Kubota, S; Li, Shuzhen; Li, Wansha; Lim, Douglas D; Lin, Haoran; Liu, Xiaochun; Luo, Yayan; Machordom, A; Martin, Andrew P; Matthysen, E; Mazzella, Maxwell N; McGeoch, Mélodie A; Meng, Zining; Nishizawa, M; O'Brien, Patricia; Ohara, M; Ornelas, Juan Francisco; Ortu, M F; Pedersen, Amy B; Preston, L; Ren, Qin; Rothhaupt, Karl-Otto; Sackett, Loren C; Sang, Qing; Sawyer, G M; Shiojiri, K; Taylor, Douglas R; Van Dongen, S; Van Vuuren, Bettine Jansen; Vandewoestijne, S; Wang, H; Wang, J T; Wang, L E; Xu, Xiang-Li; Yang, Guang; Yang, Yongping; Zeng, Y Q; Zhang, Qing-Wen; Zhang, Yongping; Zhao, Y; Zhou, Yan

    2010-01-01

    This article documents the addition of 238 microsatellite marker loci and 72 pairs of Single Nucleotide Polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Adelges tsugae, Artemisia tridentata, Astroides calycularis, Azorella selago, Botryllus schlosseri, Botrylloides violaceus, Cardiocrinum cordatum var. glehnii, Campylopterus curvipennis, Colocasia esculenta, Cynomys ludovicianus, Cynomys leucurus, Cynomys gunnisoni, Epinephelus coioides, Eunicella singularis, Gammarus pulex, Homoeosoma nebulella, Hyla squirella, Lateolabrax japonicus, Mastomys erythroleucus, Pararge aegeria, Pardosa sierra, Phoenicopterus ruber ruber and Silene latifolia. These loci were cross-tested on the following species: Adelges abietis, Adelges cooleyi, Adelges piceae, Pineus pini, Pineus strobi, Tubastrea micrantha, three other Tubastrea species, Botrylloides fuscus, Botrylloides simodensis, Campylopterus hemileucurus, Campylopterus rufus, Campylopterus largipennis, Campylopterus villaviscensio, Phaethornis longuemareus, Florisuga mellivora, Lampornis amethystinus, Amazilia cyanocephala, Archilochus colubris, Epinephelus lanceolatus, Epinephelus fuscoguttatus, Symbiodinium temperate-A clade, Gammarus fossarum, Gammarus roeselii, Dikerogammarus villosus and Limnomysis benedeni. This article also documents the addition of 72 sequencing primer pairs and 52 allele specific primers for Neophocaena phocaenoides. PMID:21565018

  4. Control químico de Diaphorina citri Kuwayama (Hemíptera: Psyllidae en lima persa Citrus latifolia Tanaka Chemical control of Diaphorina citri Kuwayama (Hemiptera: Psyllidae in Persian lime Citrus latifolia Tanaka

    Directory of Open Access Journals (Sweden)

    Luis Martín Hernández-Fuentes

    2012-06-01

    Full Text Available A nivel mundial, la citricultura representa una actividad de gran importancia. México ocupa el cuarto lugar mundial en producción. Debido a la introducción y dispersión del vector D. citri en todas las zonas citrí colas de México y la reciente detección de la enfermedad conocida como huanglongbing causada por la bacteria Candidatus Liberibacter asiaticus, es necesaria la evaluación de métodos de control de este insecto. En el año 2010 en Nayarit, México, se realizaron pruebas de efectividad técnica en campo y persistencia de productos químicos de diferente mecanismo de acción contra D. citri en lima persa. Se hicieron dos evaluaciones en fechas distintas. En cada ensayo se evaluó número de ninfas por brote y porcentaje de brotes infestados. De los productos evaluados el dimetoato, imidacloprid y la mezcla de imidacloprid más betaciflutrina ejercieron más de 85 % de control de ninfas durante 27 días después de la aplicación. En los árboles tratados con dimetoato e imidacloprid en dosis de 400 mL y 300 mL ha-¹, respectivamente, se observó 100% de brotes libres de ninfas de D. citri a los 27 días después de la aplicación. El aceite agrícola en dosis de dos y tres L ha-1 ejerció menor control de ninfas de D. citri.Citrus crops represent an outstanding activity at worldwide level. Mexico ranks fourth place in production. Due introduction and dispersion of D. citri vector in all citric zones from Mexico and the newly detected disease known as huanglongbing (HLB caused by Candidatus Liberibacter asiaticus bacteria, assessment of control methods for this bug is required. In 2010, technical effectiveness in field and persistence for different action route against Persian lime D. citri chemical products tests were performed in Nayarit, Mexico. Two assessments were made at different dates. The amount of nymphs per shoot and percentage of infested shoots were assessed in each rehearsal. From evaluated products, dimethoate, imidacloprid and the mixture of imidacloprid and (β-cyfluthrin exerted more than 85% percent of control against nymphs during 27 days after application. In trees treated with dimethoate and imidacloprid in 400 mL and 300 mL ha-1 dose, respectively, 100% of shoots free of D. citri nymphs at 27 days after application was observed. Agricultural oil in two and three L ha-1 dose exerted less control against D. citri nymphs.

  5. Discomforts of tasks video users: The case of workers of ENEA area in Bologna (Italy); I disturbi da postura riferiti da utenti di videoterminale: studio di un campione ENEA dell`area di Bologna

    Energy Technology Data Exchange (ETDEWEB)

    Cenni, P.; Varasano, R.; Cavallaro, E. [ENEA, Centro Ricerche ``E. Clementel`` Bologna (Italy). Dip. Ambiente; Arduini, R.; Mattioli, S. [Azienda USL, Bologna (Italy). Dip. di Prevenzione, Servizio di Medicina del Lavoro; Tuozzi, G. [Bologna Univ. (Italy). Dipt. di Psicologia

    1996-08-01

    The aim of this study is to investigate the subjective musculoskeletal discomfort in a sample of 334 workers (204 males and 130 females) of the ENEA area in Bologna, using for different periods and tasks video display units (VDU). On a preliminary self report questionnaire the workers have reported: hours at VDU per day, type of task type of hardware, physical-ergonomic environmental conditions, equipment and layout of the work station. Then, on a case-history form, the researcher has recorded for each subject: anamnesis, fatigue symptoms, discomfort and pain perceived in the back, shoulder, hand and arm regions. The results have shown that, in this sample, the main cause for potential musculoskeletal problems is related to aging whereas the women mainly engaged in repetitive jobs seem to be more likely subject to these troubles. About the correlation between type of task and musculoskeletal discomfort, the females refer more complaints than the males, especially when the task is repetitive. Also the number of hours spent at VDU per day is higher in the females. Finally, about physical-ergonomic environmental conditions, most users have not referred discomforts related to lighting, noise, micro climate or to work station (equipment and layout).

  6. INDAGINE GEOGRAFICA A CAMPIONE PER LO STUDIO DEI MUTAMENTI TERRITORIALI CAUSATI DALLA DIFFUSIONE DEL CTV IN UN’AREA AGRUMICOLA SICILIANA ATTRAVERSO LA GEO-LOCALIZZAZIONE DEI FOCOLAI D'INFEZIONE

    OpenAIRE

    De Cristofaro, Bernardina; Incognito, Antonella; Petino, Gianni

    2012-01-01

    Il virus della “tristeza” (Citrus Tristeza Virus CTV) ha già arrecato ingenti danni e continua a diffondersi in tutto l’areale agrumicolo italiano, in particolare in Sicilia e Calabria. Per poter tentare di arginare questa epidemia e limitarne le conseguenze è importante avere dei validi strumenti d’indagine, anche dal punto di vista geografico-economico. Tra questi, possiamo annoverare l’elaborazione di cartografie sufficientemente dettagliate di micro areali agrumicoli che ev...

  7. A study of the absorption and distribution of isotope-labelling tracer 65Zn in Typha latifolia

    International Nuclear Information System (INIS)

    The result of the study of the absorption and distribution of 65Zn in Typha latifoliate shows that Typha has a very powerful capacity of absorbing 65Zn. The 65Zn absorbed and accumulated by the root and rhizome of Typha was much greater than that transferred to the leaf. The capacity of absorbing 65Zn of the organs of Typha is as follows: root>rhizome>leaf. The research also shows that the quantity of 65Zn absorbed by Typha increased with the time. Moreover, the quantity of zinc absorbed by Typha increased with the concentration of 65Zn in the culture solution. But the rate of absorbing 65Zn of Typha declined after 24 hor for the concentration of 65Zn in culture solution beyond 200 mg·kg-1

  8. Ethanol production from mahula (Madhuca latifolia L.) flowers with immobilized cells of Saccharomyces cerevisiae in Luffa cylindrica L. sponge discs

    Energy Technology Data Exchange (ETDEWEB)

    Behera, Shuvashish; Mohanty, Rama Chandra [Department of Botany, Utkal University, Vanivihar, Bhubaneswar 751 004, Orissa (India); Ray, Ramesh Chandra [Microbiology Laboratory, Central Tuber Crops Research Institute (Regional Centre), Bhubaneswar 751 019, Orissa (India)

    2011-01-15

    The dried spongy fruit of luffa (Luffa cylindrica L.), a cucurbitaceous crop available in abundance in tropical and sub-tropical countries has been found to be a promising material for immobilizing microbial cells. The aim of the present study was to examine the ethanol production from mahula flowers in submerged fermentation using whole cells of Saccharomyces cerevisiae immobilized in luffa sponge discs. The cells not only survived but also were physiologically active in three more cycles of fermentation without significant reduction (<5%) in ethanol production. After 96 h, there was 91.1% sugar conversion producing 223.2 g ethanol/kg flowers (1st cycle) which was 0.99%, 2.3% and 3.2% more than 2nd (221 g ethanol/kg flowers), 3rd (218 g ethanol/kg flowers) and 4th (216 g ethanol/kg flowers) cycle of fermentation, respectively. Furthermore, ethanol production by immobilized cells was 8.96% higher than the free cells. (author)

  9. LC-MS and 1H NMR as an improved dereplication tool to identify antifungal diterpenoids from Sagittaria latifolia

    Science.gov (United States)

    A dereplication strategy using a combination of liquid chromatography-mass spectrometry (LC-MS) and proton nuclear magnetic resonance spectroscopy (1H NMR) to facilitate compound identification towards antifungal natural product discovery is presented. This analytical approach takes advantage of th...

  10. Evaluación de patrones para lima tahití (Citrus latifolia Tanaka) frente a Citrus tristeza virus (CTV)

    OpenAIRE

    Beltrán Montoya, Johnny Camilo

    2012-01-01

    En el presente trabajo se evaluó la respuesta de patrones para lima Tahití frente a infecciones de Citrus tristeza virus. El documento se divide en tres capítulos. El primer capítulo es una revisión general del estado del arte de CTV, que abarca generalidades sobre sus hospederos, distribución, genoma viral, síntomas que generan las diferentes cepas y antecedentes de lima Tahití en Colombia. El segundo capítulo abarca la identificación de cepas de CTV que infectan en campo mediante el uso de ...

  11. ВЛИЯНИЕ РОГОЗА ШИРОКОЛИСТНОГО (TYPHA LATIFOLIA L.) И КОМПОНЕНТОВ ФИЛЬТРУЮЩЕЙ ЗАГРУЗКИ НА ЭФФЕКТИВНОСТЬ УДАЛЕНИЯ СОЕДИНЕНИЙ АЗОТА В СИСТЕМАХ ПОЧВЕННО-БОЛОТНОЙ ОЧИСТКИ СТОЧНЫХ ВОД

    OpenAIRE

    Сивкова, Елена; Прибыткова, Екатерина

    2011-01-01

    Изучено влияние рогоза широколистного (Typha latifolia) и наличия известняка в составе фильтрующей загрузки площадок почвенно-болотной очистки сточных вод на процессы удаления соединений азота. Показано изменение содержания ионов аммония в воде, вычислены скорость и эффективность удаления NH4 + в зависимости от условий очистки. Установлено, что присутствие высшей водной растительности в системах очистки горизонтального поверхностного потока повышает скорость и эффективность удаления аммонийно...

  12. Derivatization of phytochelatins from Silene vulgaris, induced upon exposure to arsenate and cadmium: comparison of derivatization with Ellman's reagent and monobromobimane.

    Science.gov (United States)

    Sneller, F E; van Heerwaarden, L M; Koevoets, P L; Vooijs, R; Schat, H; Verkleij, J A

    2000-09-01

    Phytochelatins (PCs) are a family of thiol-rich peptides, with the general structure (gamma-Glu-Cys)(n)()-Gly, with n = 2-11, induced in plants upon exposure to excessive amounts of heavy metals and some metalloids, such as arsenic. Two types of PC analyses are currently used, i.e., acid extraction and separation on HPLC with either precolumn derivatization (pH 8.2) with monobromobimane (mBBr) or postcolumn derivatization (pH 7.8) with Ellman's reagent [5, 5'-dithiobis(2-nitrobenzoic acid), DTNB]. Although both methods were satisfactory for analysis of Cd-induced PCs, formation of (RS)(3)-As complexes during extraction of As-induced PCs rendered the DTNB method useless. This paper shows that precolumn derivatization with mBBr, during which the (RS)(3)-As complexes are disrupted, provides a qualitative and quantitative analysis of both Cd- and As-induced PCs. In addition, derivatization efficiencies of both methods for the oligomers with n = 2-4 (PC(2)(-)(4)) are compared. Derivatization efficiency decreased from 71.8% and 81.4% for mBBr and DTNB derivatization, respectively, for PC(2) to 27.4% and 50.2% for PC(4). This decrease is most likely due to steric hindrance. Correction of measured thiol concentration is therefore advised for better quantification of PC concentrations in plant material.

  13. Derivatization of phytochelatins from Silene vulgaris, induced upon exposure to arsenate and cadmium: comparison of derivatization with Ellman's reagent and monobromobimane.

    Science.gov (United States)

    Sneller, F E; van Heerwaarden, L M; Koevoets, P L; Vooijs, R; Schat, H; Verkleij, J A

    2000-09-01

    Phytochelatins (PCs) are a family of thiol-rich peptides, with the general structure (gamma-Glu-Cys)(n)()-Gly, with n = 2-11, induced in plants upon exposure to excessive amounts of heavy metals and some metalloids, such as arsenic. Two types of PC analyses are currently used, i.e., acid extraction and separation on HPLC with either precolumn derivatization (pH 8.2) with monobromobimane (mBBr) or postcolumn derivatization (pH 7.8) with Ellman's reagent [5, 5'-dithiobis(2-nitrobenzoic acid), DTNB]. Although both methods were satisfactory for analysis of Cd-induced PCs, formation of (RS)(3)-As complexes during extraction of As-induced PCs rendered the DTNB method useless. This paper shows that precolumn derivatization with mBBr, during which the (RS)(3)-As complexes are disrupted, provides a qualitative and quantitative analysis of both Cd- and As-induced PCs. In addition, derivatization efficiencies of both methods for the oligomers with n = 2-4 (PC(2)(-)(4)) are compared. Derivatization efficiency decreased from 71.8% and 81.4% for mBBr and DTNB derivatization, respectively, for PC(2) to 27.4% and 50.2% for PC(4). This decrease is most likely due to steric hindrance. Correction of measured thiol concentration is therefore advised for better quantification of PC concentrations in plant material. PMID:10995306

  14. The genetic controlled hydroxylation pattern of the anthocyanin b-ring in Silene dioica is not determined at the p-coumaric acid stage

    NARCIS (Netherlands)

    Heinsbroek, R.; Brederode, J. van

    1980-01-01

    The basic C-15 skeleton of flavonoids is formed by the head-to-tail condensation of three malonyl-CoA units to one molecule of hydroxycinnamoyl-CoA. The A-ring and part of the heterocyclic ring are derived from the acetate units, the B-ring from the hydroxycinnamoyl ester. The basic C-15 flavonoid i

  15. Wild vegetable mixes sold in the markets of Dalmatia (southern Croatia

    Directory of Open Access Journals (Sweden)

    Łuczaj Łukasz

    2013-01-01

    Full Text Available Abstract Background Dalmatia is an interesting place to study the use of wild greens as it lies at the intersection of influence of Slavs, who do not usually use many species of wild greens, and Mediterranean culinary culture, where the use of multiple wild greens is common. The aim of the study was to document the mixtures of wild green vegetables which are sold in all the vegetable markets of Dalmatia. Methods All vendors (68 in all 11 major markets of the Dalmatian coast were interviewed. The piles of wild vegetables they sold were searched and herbarium specimens taken from them. Results The mean number of species in the mix was 5.7. The most commonly sold wild plants are: Sonchus oleraceus L., Allium ampeloprasum L., Foeniculum vulgare Mill., Urospermum picroides F.W.Schmidt, Papaver rhoeas L., Daucus carota L., Taraxacum sp., Picris echioides L., Silene latifolia Poir. and Crepis spp. Also the cultivated beet (Beta vulgaris L. and a few cultivated Brassicaceae varieties are frequent components. Wild vegetables from the mix are usually boiled for 20–30 minutes and dressed with olive oil and salt. Altogether at least 37 wild taxa and 13 cultivated taxa were recorded. Apart from the mixes, Asparagus acutifolius L. and Tamus communis L. shoots are sold in separate bunches (they are usually eaten with eggs, as well as some Asteraceae species, the latter are eaten raw or briefly boiled. Conclusions The rich tradition of eating many wild greens may result both from strong Venetian and Greek influences and the necessity of using all food resources available in the barren, infertile land in the past. Although the number of wild-collected green vegetables is impressive we hypothesize that it may have decreased over the years, and that further in-depth local ethnobotanical studies are needed in Dalmatia to record the disappearing knowledge of edible plants.

  16. Multiple infections by the anther smut pathogen are frequent and involve related strains.

    Directory of Open Access Journals (Sweden)

    Manuela López-Villavicencio

    2007-11-01

    Full Text Available Population models of host-parasite interactions predict that when different parasite genotypes compete within a host for limited resources, those that exploit the host faster will be selected, leading to an increase in parasite virulence. When parasites sharing a host are related, however, kin selection should lead to more cooperative host exploitation that may involve slower rates of parasite reproduction. Despite their potential importance, studies that assess the prevalence of multiple genotype infections in natural populations remain rare, and studies quantifying the relatedness of parasites occurring together as natural multiple infections are particularly scarce. We investigated multiple infections in natural populations of the systemic fungal plant parasite Microbotryum violaceum, the anther smut of Caryophyllaceae, on its host, Silene latifolia. We found that multiple infections can be extremely frequent, with different fungal genotypes found in different stems of single plants. Multiple infections involved parasite genotypes more closely related than would be expected based upon their genetic diversity or due to spatial substructuring within the parasite populations. Together with previous sequential inoculation experiments, our results suggest that M. violaceum actively excludes divergent competitors while tolerating closely related genotypes. Such an exclusion mechanism might explain why multiple infections were less frequent in populations with the highest genetic diversity, which is at odds with intuitive expectations. Thus, these results demonstrate that genetic diversity can influence the prevalence of multiple infections in nature, which will have important consequences for their optimal levels of virulence. Measuring the occurrence of multiple infections and the relatedness among parasites within hosts in natural populations may be important for understanding the evolutionary dynamics of disease, the consequences of vaccine use

  17. Lower prevalence but similar fitness in a parasitic fungus at higher radiation levels near Chernobyl.

    Science.gov (United States)

    Aguileta, Gabriela; Badouin, Helene; Hood, Michael E; Møller, Anders P; Le Prieur, Stephanie; Snirc, Alodie; Siguenza, Sophie; Mousseau, Timothy A; Shykoff, Jacqui A; Cuomo, Christina A; Giraud, Tatiana

    2016-07-01

    Nuclear disasters at Chernobyl and Fukushima provide examples of effects of acute ionizing radiation on mutations that can affect the fitness and distribution of species. Here, we investigated the prevalence of Microbotryum lychnidis-dioicae, a pollinator-transmitted fungal pathogen of plants causing anther-smut disease in Chernobyl, its viability, fertility and karyotype variation, and the accumulation of nonsynonymous mutations in its genome. We collected diseased flowers of Silene latifolia from locations ranging by more than two orders of magnitude in background radiation, from 0.05 to 21.03 μGy/h. Disease prevalence decreased significantly with increasing radiation level, possibly due to lower pollinator abundance and altered pollinator behaviour. Viability and fertility, measured as the budding rate of haploid sporidia following meiosis from the diploid teliospores, did not vary with increasing radiation levels and neither did karyotype overall structure and level of chromosomal size heterozygosity. We sequenced the genomes of twelve samples from Chernobyl and of four samples collected from uncontaminated areas and analysed alignments of 6068 predicted genes, corresponding to 1.04 × 10(7)  base pairs. We found no dose-dependent differences in substitution rates (neither dN, dS, nor dN/dS). Thus, we found no significant evidence of increased deleterious mutation rates at higher levels of background radiation in this plant pathogen. We even found lower levels of nonsynonymous substitution rates in contaminated areas compared to control regions, suggesting that purifying selection was stronger in contaminated than uncontaminated areas. We briefly discuss the possibilities for a mechanistic basis of radio resistance in this nonmelanized fungus. PMID:27136128

  18. Web-Based Dynamic Assessment: Taking Assessment as Teaching and Learning Strategy for Improving Students e-Learning Effectiveness

    Science.gov (United States)

    Wang, Tzu-Hua

    2010-01-01

    This research combines the idea of cake format dynamic assessment defined by Sternberg and Grigorenko (2001) and the "graduated prompt approach" proposed by (Campione and Brown, 1985) and (Campione and Brown, 1987) to develop a multiple-choice Web-based dynamic assessment system. This research adopts a quasi-experimental design to investigate the…

  19. Effects of Dietary Carbohydrate Replaced with Wild Rice (Zizania latifolia (Griseb Turcz on Insulin Resistance in Rats Fed with a High-Fat/Cholesterol Diet

    Directory of Open Access Journals (Sweden)

    Chengkai Zhai

    2013-02-01

    Full Text Available Wild rice (WR is a very nutritious grain that has been used to treat diabetes in Chinese medicinal practice. City diet (CD is based on the diet consumed by Asian area residents in modern society, which is rich in saturated fats, cholesterol and carbohydrates. The present study was aimed at evaluating the effects of replacing white rice and processed wheat starch of CD with WR as the chief source of dietary carbohydrates on insulin resistance in rats fed with a high-fat/cholesterol diet. Except the rats of the low-fat (LF diet group, the rats of the other three groups, including to high-fat/cholesterol (HFC diet, CD and WR diet, were fed with high-fat/cholesterol diets for eight weeks. The rats fed with CD exhibited higher weight gain and lower insulin sensitivity compared to the rats consuming a HFC diet. However, WR suppressed high-fat/cholesterol diet-induced insulin resistance. WR decreased liver homogenate triglyceride and free fatty acids levels, raised serum adiponectin concentration and reduced serum lipocalin-2 and visfatin concentrations. In addition, the WR diet potently augmented the relative expressions of adiponectin receptor 2, peroxisome proliferator-activated receptors, alpha and gamma, and abated relative expressions of leptin and lipocalin-2 in the tissues of interest. These findings indicate that WR is effective in ameliorating abnormal glucose metabolism and insulin resistance in rats, even when the diet consumed is high in fat and cholesterol.

  20. Soil Characteristics and Lodgepole Pine (Pinus contorta var. latifolia Performance Two Decades after Disk Trenching of Unburned and Broadcast-Burned Plots in Subboreal British Columbia

    Directory of Open Access Journals (Sweden)

    Jacob O. Boateng

    2011-01-01

    Full Text Available We examined the effects of low-impact broadcast-burning and disk-trenching planting position (control, hinge, trench on soil characteristics and lodgepole pine foliar nutrition and growth over two decades at a subboreal site in British Columbia, Canada. Broadcast burning had virtually no effect on either the bulk density or chemical properties of soil. In contrast, significant reductions in soil bulk density and increases in soil nutrient availability persisted for 20 years in hinge position soils relative to undisturbed (control soil between trenches. These effects on bulk density and nutrient availability are associated with significant differences in pine size by year 6. Burning and planting positions interacted significantly in their effect on pine height, diameter, and stem volume for at least 19 years. Pine survival was high regardless of burning or planting position. Neither broadcast burning nor planting position significantly affected lodgepole pine foliar nutrient status in this study.

  1. Effects of introgression on the genetic population structure of two ecologically and economically important conifer species: lodgepole pine (Pinus contorta var. latifolia) and jack pine (Pinus banksiana).

    Science.gov (United States)

    Cullingham, Catherine I; Cooke, Janice E K; Coltman, David W

    2013-10-01

    Forest trees exhibit a remarkable range of adaptations to their environment, but as a result of frequent and long-distance gene flow, populations are often only weakly differentiated. Lodgepole and jack pine hybridize in western Canada, which adds the opportunity for introgression through hybridization to contribute to population structure and (or) adaptive variation. Access to large sample size, high density SNP datasets for these species would improve our ability to resolve population structure, parameterize introgression, and separate the influence of demography from adaptation. To accomplish this, 454 transcriptome reads for lodgepole and jack pine were assembled using Newbler and MIRA, the assemblies mined for SNPs, and 1536 SNPs were selected for typing on lodgepole pine, jack pine, and their hybrids (N = 536). We identified population structure using both Bayesian clustering and discriminate analysis of principle components. Introgressed SNP loci were identified and their influence on observed population structure was assessed. We found that introgressed loci resulted in increased differentiation both within lodgepole and jack pine populations. These findings are timely given the recent mountain pine beetle population expansion in the hybrid zone, and will facilitate future studies of adaptive traits in these ecologically important species. PMID:24237338

  2. Effect of N:P ratio of influent on biomass, nutrient allocation, and recovery of Typha latifolia and Canna 'Bengal Tiger' in a laboratory-scale constructed wetland

    Science.gov (United States)

    Constructed wetlands (CWs) are an effective low-technology approach for treating agricultural, industrial, and municipal wastewater. Recovery of phosphorous by constructed wetland plants may be affected by wastewater nitrogen to phosphorous (N:P) ratios. Varying N:P ratios were supplied to Canna '...

  3. Cultivation of Agaricus bisporus by Adding Sheathing Leaves of Zizania latifolia%添加茭白鞘叶栽培双孢蘑菇初探

    Institute of Scientific and Technical Information of China (English)

    蔡志英; 卢政辉; 丁中文; 曾辉

    2012-01-01

    The sheathing leaves of Zizania lati folia, which contained 4 times higher in organic matters and twice more nitrogen than the rice straw, was seen as an excellent potential substrate material for mushroom cultivation. Our experiment by adding the sheathing leaves of Z. lati folia to the traditional compost formulation in growing Agaricus bisporus indicated that increasing the sheathing leaves in the medium slowed the hyphae running, delayed the pinning to 3 days, and postponed the mushroom harvest up to 4 days. The optimal results appeared to be when 50% sheathing leaves and 50% rice straw were used in combination for the formulation. Using the formulation, (a) the mushroom yield in one growing season reached 20. 45 kg o m-2, approaching the same level as the traditional formula (Control) ; (b) the output/input ratio was 4. 479, which was higher than 3. 439 for Control) and, (c) there was no appreciable differences from Control on the single fruit weight or the characteristics of the fruiting body. Thus, the substitution seemed economically viable. And, at the same time, the practice could alleviate the rice straw shortage in major mushroom producing areas, and materially reduce waste and pollution caused by the disposal of the sheathing leaves.%检测表明茭白鞘叶中含有丰富的有机质与氮源,分别是稻草的2倍和4倍,是栽培双孢蘑菇的理想替代原料.在传统稻草配方中添加茭白鞘叶栽培蘑菇的试验,研究结果表明:走菌速度随配方中茭白鞘叶含量的增加而减慢,茭白鞘叶配方的蘑菇现蕾期和开始采菇时间分别比稻草配方迟3、4 d;稻草与茭白鞘叶各占50%配方为最佳,单季产量达到20.45 kg·m-2,几乎和传统配方持平,而投入产出比可以达到4.479,超过传统配方的3.439,并且菇体单粒重和传统配方没有显著差异,而且鲜菇的品质经分析和传统配方几乎无异,具有良好的经济效益.本研究初步明确了茭白鞘叶栽培蘑菇的工艺流程、栽培技术与克服出现问题的方法,而且利用茭白鞘叶栽培蘑菇既缓解了蘑菇主产区稻草紧缺的局面,又是解决茭白鞘叶资源浪费和环境污染的可行途径.

  4. Contexto y Caracterización de la Cadena de Suministro del Limón Persa (Citrus latifolia Tanaka en Veracruz-México

    Directory of Open Access Journals (Sweden)

    Gregorio Fernández-Lambert

    2015-01-01

    Full Text Available Esta investigación se realiza en el Distrito Citrícola de Martínez de la Torre, Ver., con alcance a los municipios de Álamo Temapache, y Cuitláhuac en Veracruz, y reporta el contexto e integración de la cadena de suministro del limón persa. El estudio de sus eslabones permitió identificar las problemáticas de abastecimiento, operación y distribución en dicha cadena. A partir de entrevistas abiertas no estructuradas a especialistas, técnicos, productores y empresarios, así como la consulta de bases de datos y documentos de organismos afines a esta agroindustria, los resultados de la investigación advierten que la fuente de los problemas estructurales de la ineficacia en la sincronización de la cadena de suministro del limón persa, se centra en la baja productividad del huerto, un excesivo intermediarismo entre el huerto y la exportadora, la baja adopción tecnológica o uso de tecnología hechiza en los procesos de empaque del cítrico.

  5. A comparative experimental evaluation of anti-inflammatory activity of Premna obtusifolia Linn and Premna latifolia Roxb leaves in Charles foster rats

    OpenAIRE

    Kumari, Harshitha; Shrikanth, P.; Chaithra; Pushpan, Reshmi; Nishteswar, K.

    2011-01-01

    Agnimantha, a classical Ayurvedic drug is one among the dashamoolas, i.e., group of ten plants whose roots form the useful part. It is a main ingredient of many Ayurvedic preparations. Various source plants from the genus Clerodendrum and Premna are used in different regions of the country. In this study, two species of Premna were selected and evaluated for anti-inflammatory activity against carrageenan induced rat hind paw edema. The selected animals were divided into four groups and test d...

  6. Effects of litter on substrate conditions and growth of emergent macrophytes

    NARCIS (Netherlands)

    Van der Putten, W.H.; Peters, B.A.M.; Van den Berg, R.S.

    1997-01-01

    Three successive emergent macrophytes (Typha latifolia L., Phragmites australis (Cav.) Steudel and Glyceria maxima (Hartman) Holmbly) were each grown in substrates collected from three different zones of shoreline vegetation development (non- vegetated sediment, the interface between T. latifolia an

  7. Studio osservazionale comparativo su un campione di studenti del Nord, del Centro e del Sud con valutazione della Maturità, del Test di accesso e delle scelte di sede effettuate al concorso con graduatoria nazionale 2013-2014. Osservazioni preliminari.

    OpenAIRE

    Familiari, G; Baldini, R; Lanzone, A; Valli, M; Di Liegro, I; Locatelli, V; Morini, S; Muraro, R; Strepparava, MG; Cavaggioni, G; Barbaranelli, C.; Heyn, R.; Relucenti, M; Gaudio, E.

    2014-01-01

    In compliance with the new norms governing access to medical faculties in Italy, which foresee a sole national ranking list, a comparative observational study was carried out on a sample of students from the country’s northern, central and southern areas (888 in all), the aim of which was to appraise the students’ O-level marks, the results of their national entrance examinations and the particular university campuses chosen by them. The preliminary results, based on a direct survey carrie...

  8. Comportamenti sessuali e rischio di AIDS in studenti universitari

    OpenAIRE

    M.L. Sammarco; G. Ripabelli; I. Fanelli; G.M. Grasso

    2003-01-01

    Obiettivi: la trasmissione eterosessuale di HIV sta acquisendo una sempre maggiore importanza. Obiettivo del presente studio è stato, pertanto, quello di valutare in un campione di studenti universitari le caratteristiche dei comportamenti sessuali a rischio.

    Metodi: nell’ambito di uno studio più ampio, è stato distribuito ad un campione di studenti dell’Università del Molise (Campobasso) un questionario anon...

  9. 不同直径微孔保鲜袋保鲜茭白效果研究%Quality assessment of few-flower wildrice (zizania latifolia turcz.) packed with perforated polypropylene bag with different orifice diameter during cold storage

    Institute of Scientific and Technical Information of China (English)

    钱炳俊; 邓云; 陈骏; 张晗; 沈永培; 平健; 曹林奎

    2011-01-01

    以带壳鲜茭白为试材,用5种不同孔径的聚乙烯袋进行包装处理,在(2±1)℃和90%~95%RH下贮藏42d后,分析茭白木质素、丙二醛、Vc、可溶性回形物、pH、表皮色泽等品质变化.结果表明:不同孔径的聚乙烯袋对茭白的品质指标的影响存在差异,其中普通聚乙烯袋对茭白表皮的绿化、纤维化、木质化、Vc降解、失重和腐烂等的延缓作用比其余4种包装袋更为显著(P<0.05).这表明普通聚乙烯保鲜袋更适合带壳茭白保鲜.%The few-flower wildrice {Zizania aquaiica L.) packed with perforated polypropylene bags with 5 different orifice diameters were stored at (2±l)"Cand 90%~95% relative humidity. After 42 days, main physicochemical and sensorial quality changes were evaluated. The results showed that the orifice diameter is a factor affecting the quality of few-flower wildrice. Common polypropylene bag was more efficient for inhibiting greening and lignifying. Decreasing degradation of Vc and weight loss, reducing brown and decay than the other 4 bags.

  10. 茭白线粒体蛋白双向电泳体系建立%Establishment of two-dimensional electrophoresis system for mitochondrial proteins of Zizania latifolia

    Institute of Scientific and Technical Information of China (English)

    杜传来; 罗海波; 彭昕; 王韦华; 郁志芳

    2016-01-01

    [目的]建立适合于茭白线粒体蛋白质组分析的双向电泳技术体系,为进一步开展茭白线粒体差异蛋白质组学研究提供参考.[方法]以茭白为试验材料,比较不同线粒体提取纯化方法、IPG胶条pH范围及蛋白上样量等条件对双向电泳图谱的影响.[结果]差速离心(3000~14000×g)联合Percoll不连续密度梯度离心(20%∶24%∶40%=2∶4∶2)对茭白线粒体的提取纯化效果优于仅采用差速离心.采用改良酚抽法提取茭白线粒体蛋白,17 cm、pH 3~10的IPG胶条,1.0 mg上样量,12% SDS-PAGE进行双向电泳,0.12%考马斯亮蓝G-250胶体考染法染色,茭白线粒体蛋白主要分布在pH 4~8范围内,pH 3~4和pH 8~10范围内蛋白点较少;进一步选用pH 4~7和pH 5~8的IPG胶条对茭白线粒体蛋白进行双向电泳,分离效果均明显提高,且pH 4~7范围内IPG胶条的双向电泳图谱清晰,蛋白点分辨率较高.分别采用0.8、1.0和1.2 mg 3个不同的上样量进行双向电泳,结果表明上样量为1.0 mg时,电泳图谱质量最好.[结论]通过差速离心联合Percoll不连续密度梯度离心提取纯化茭白线粒体,并控制好双向电泳体系的pH范围、上样量等因素,可获得蛋白质点清晰、分辨率高、重复性好的双向电泳图谱.

  11. Progress on sex differentiation in unisexual flower plants%单性花植物性别分化研究进展

    Institute of Scientific and Technical Information of China (English)

    高暝; 陈益存; 杨素素; 刘英冠; 朱慧萍; 汪阳东

    2015-01-01

    性别分化是生物界普遍存在的一个自然现象。单性花由于仅含一种有功能的性器官,通过对其研究有助于解释植物性别分化和性别决定调控机制。植物性别决定方式多样且复杂,既有通过性别决定基因决定性别,又有性染色体,通过阻止性别决定基因重组确保稳定的性别分离;同时表观遗传由于影响基因表达活性,对性别分化也发挥重要作用。此外,植物激素、遗传因子、表观遗传修饰等之间存在相互作用,共同决定单性花性别。本文从单性花分类、雌雄花表型性状差异、遗传基础、表观遗传修饰、激素调控等方面综述单性花性别分化和决定调控机理,并提出未来研究中将面临的挑战和应对策略,为揭示植物性别分化和决定的机理提供有效参考。%Sexual reproduction is a universal phenomenon in the world of eukaryotes,and sex differentiation and determination are major events in the evolutionary history of angiosperms.The production of unisexual flowers has evolved numerous times from hermaphroditic ancestors,resulting in dioecious (5%)and monoecious (7%) plant taxa.Through developmental process,two broad categories of unisexual flowers have been recognized;the former results from flowers that are bisexual at initiation and become unisexual by termination of the devel-opment of the androecium (or the male reproductive organs)or gynoecium (or the female reproductive organs) and the latter results from sex differentiation occurring before initiation of stamens and carpels.Attempts have been made to understand regulatory mechanisms for plant sex determination by investigating unisexual flowers. The determinants of sexual phenotype in plants are diverse and complicated,including sex-determining genes in monoecious plants,Zea mays (corn)and Cucumis sp.(cucumber and its relatives);sex chromosomes in Silene latifolia and Carica papaya ,which ensure the stable

  12. Phytoecdysteroids of the East Asian Caryophyllaceae

    Directory of Open Access Journals (Sweden)

    Elena Novozhilova

    2015-01-01

    Full Text Available Background: Occurrence of integristerone A (1, 20-hydroxyecdysone (2, ecdysone (3, 2-deoxy-20-hydroxyecdysone (4 has been analyzed in 64 species of the East Asian Caryophyllaceae. Materials and Methods: Ecdysteroid content was determinate by high-performance liquid chromatography (HPLC. HPLC with a high-resolution mass spectrometry was performed on Shimadzu LCMS-IT-TOF (Japan system equipped with a LC-20A Prominence liquid chromatograph, a photodiode array detector SPD-M20A and ion-trap/time-of-flight mass spectrometer. Results: New sources of phytoecdysteroids: Melandrium sachalinense and Melandrium firmum have been revealed. It is the 1 st time that two has been identified in M. sachalinense and M. firmum; 1 in the species: Lychnis fulgens, Silene repens, Silene foliosa, Silene stenophylla, Silene jenisseensis and M. sachalinense; 3 in Lychnis cognata; 4 in L. fulgens, S. stenophylla and S. jenisseensis (the tribe Lychnideae, the subfamily Caryophylloideae. Ecdysteroid-negative taxa are Spergularia rubra of the tribe Sperguleae; species of the genera Minuartia, Honckenya, Eremogone, Arenaria, Moehringia, Pseudostellaria, Fimbripetalum, Stellaria and Cerastium of the tribe Alsineae; Scleranthus annuus of the tribe Sclerantheae, as well as the East Asian representatives of the genera Gypsophila, Psammophiliela, Dianthus and Saponaria of the tribe Diantheae; Oberna and Agrostemma of the tribe Lychnideae. Conclusion: This investigation shows the most promising sources of ecdysteriods are species of genera Silene and Lychnis.

  13. Shakespeare, plant blindness and electronic media

    Science.gov (United States)

    Use is made of a published (2005) survey of botanical literacy in modern London: Ten very common wild flowers (ragwort, cow parsley, foxglove, red campion, germander speedwell, primrose, lesser celandine, common dog violet, common daisy, and greater plantain) were seldom recogonized by A-level stude...

  14. Effects of Lactic Acid Bacteria and Wild rice Bran Supplementation on Silage Quality of Few-flower Wild rice (Zizania latifolia Turcz) Leaf Sheath%添加乳酸菌和米糠对茭白鞘叶青贮品质的影响

    Institute of Scientific and Technical Information of China (English)

    朱雯; 郭海明; 张勇; 叶均安

    2015-01-01

    本文旨在研究乳酸菌、米糠、和水分添加量对青贮茭白鞘叶质量的影响.采用L9(34)正交试验设计,茭白鞘叶青贮时,分别添加乳酸菌(0、2×1 05、4×105 CFU/kg)、米糠(0、2%、4% DM)和水(0、7.5%、15% DM),贮存60 d后,感官评定茭白鞘叶的青贮质量,测定浸提液的pH值、氨态氮和有机酸的含量.结果表明:乳酸菌的添加提高了青贮发酵的感官评定等级(P<0.05),米糠和水分含量对青贮发酵的感官评定无影响(P>0.05);各处理组之间pH值和氨态氮占总氮比差异均不显著(P>0.05);乳酸菌的添加提高了乙酸和丙酸的浓度(P<0.05);米糠的添加提高了丙酸的浓度(P<0.05);乳酸浓度、丁酸浓度和乳酸占总酸比各处理之间差异不显著(P>0.05).综上所述,茭白鞘叶在无任何添加剂的情况下,也可以青贮,但是青贮质量一般.乳酸菌的添加显著提高了青贮品质,利于提高青贮发酵的有氧稳定性;米糠的添加量为2%~4%时,提高了青贮品质,但是未达到显著水平;茭白鞘叶水分含量在63%~71%之间时对青贮质量无显著影响.

  15. Caracterização da composição química e do rendimento dos resíduos industriais do limão Tahiti (Citrus latifolia Tanaka Chemical composition and Tahiti lime industrial residues output characterization

    Directory of Open Access Journals (Sweden)

    Luciana Maria Vieira Lopes Mendonça

    2006-12-01

    Full Text Available A indústria de sucos utiliza apenas de 40% a 50% dos frutos, sendo o restante considerado resíduo industrial. Estes resíduos apresentam-se ricos em alguns componentes como pectina, vitamina C e fibras dietárias que o tornam matéria-prima para a indústria alimentícia, farmacêutica e de rações. Visando o aproveitamento dos resíduos industriais do limão Tahiti para tais fins, caracterizou-se a composição centesimal, os teores de fibras, de pectina e de vitamina C presentes nas frações flavedo, albedo e bagaço do fruto. A partir da avaliação dos dados pelo teste de Scott Knott 5% de probabilidade, observou-se que os teores de cinzas, pectina, proteína, vitamina C e fibra bruta foram diferentes no flavedo, albedo e bagaço. O resíduo industrial do limão Tahiti tem um grande potencial para uso em formulação de rações para animais, dada a sua composição química, assim como poderá ser usado para a extração de fibras, pectina, vitamina C e óleos.The juice industry makes use of only 40 to 50% of the fruit and its remainder is considered industrial residue. This residue is very rich in some of its compounds such as pectin, ascorbic acid and dietary fibers making it into raw material for the food industry, pharmaceutics and animal food. With the purpose of using the industrial residue of Tahiti lime for such purposes, the centesimal composition, fiber contents, pectin and vitamin C contained in flavedo fractions, albedo and fruit pulp were characterized. From the evaluation for the Scott Knott's test to 5% of probability, it was observed that the ash content, pectin, protein, vitamin C and rude fiber were different for flavedo, albedo and pulp. Tahiti lime industrial residue has a great potential for animal food formulation, considering its chemical composition, as well as the fact that it can be used for fiber extraction, pectin, vitamin C and oils.

  16. Environ: E00555 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available rida) Obtained by steam distillation Lavandin is a cross between true lavender (L.angustifolia) and spike la...ifolia [TAX:39329] / L. latifolia [TAX:39331]) The fresh aerial part of flowering of lavandin (Lavandula hyb

  17. Environ: E00729 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available [CPD:C05851], 8-para-Cymenol Lavandula latifolia [TAX:39331] Lamiaceae (mint fam...844] Essential oils [BR:br08321] Dicot plants: asterids Lamiaceae (mint family) E00729 Spike oil CAS: 8016-78-2 ...

  18. Evaluation of antidiabetic activity of plants used in Western Sudan

    Directory of Open Access Journals (Sweden)

    Maha Abdulrahman Alamin

    2015-05-01

    Conclusions: This study demonstrated that T. bakis, N. latifolia, R. nilotica and Mitragyna inremis have therapeutic value in diabetes and related complications and thus supporting the traditional uses of these plants in Sudanese traditional medicine.

  19. Glyphosate translocation in herbicide tolerant plants Translocação do glyphosate em plantas tolerantes ao herbicida

    OpenAIRE

    L Galon; E.A. Ferreira; I. Aspiazú; G. Concenço; A.F. Silva; Silva, A. A.; Vargas, L.

    2013-01-01

    The objective of this study was to evaluate glyphosate translocation in glyphosate-tolerant weed species (I. nil, T. procumbens and S. latifolia) compared to glyphosate-susceptible species (B. pilosa). The evaluations of 14C-glyphosate absorption and translocation were performed at 6, 12, 36 and 72 hours after treatment (HAT) in I. nil and B. pilosa, and only at 72 HAT in the species T. procumbens and S. latifolia. The plants were collected and fractionated into application leaf, other leaves...

  20. Adjuvants for single droplet application of glyphosate

    DEFF Research Database (Denmark)

    Mathiassen, Solvejg Kopp; Kudsk, Per; Lund, Ivar

    2016-01-01

    Retention and biological activity of droplets of glyphosate deposited onto plant leaves using a Drop on Demand inkjet printer application system, was examined on pot-grown Brassica napus, Solanum nigrum, Chenopodium album, Silene noctiflora and Echinocloa crus-galli plants. Retention was measured...... but the biological activity of glyphosate was not improved....

  1. Adjuvants for single droplet application of glyphosate

    DEFF Research Database (Denmark)

    Mathiassen, Solvejg K.; Kudsk, Per; Lund, Ivar

    Retention and biological activity of droplets of glyphosate deposited onto plant leaves using a Drop on Demand inkjet printer application system, was examined on pot-grown Brassica napus, Solanum nigrum, Chenopodium album, Silene noctiflora and Echinocloa crus-galli plants. Retention was measured...... but the biological activity of glyphosate was not improved....

  2. Reliability of a novel paradigm for determining hemispheric lateralization of visuospatial function

    NARCIS (Netherlands)

    Whitehouse, A.J.O.; Badcock, N.A.; Groen, M.A.; Bishop, D.V.M.

    2009-01-01

    In most individuals, language production and visuospatial skills are subserved predominantly by the left and right hemispheres, respectively. Functional Transcranial Doppler (fTCD) provides a noninvasive and relatively low-cost method for measuring functional lateralization. However, while the silen

  3. Analisi dell’anisotropia microstrutturale in materiali compositi rinforzati con fibre corte

    Directory of Open Access Journals (Sweden)

    D. Dreossi

    2008-01-01

    Full Text Available La microtomografia con luce di sincrotrone si è rivelata una tecnica particolarmente efficace per l’analisi della struttura risultante dalla distribuzione degli orientamenti assunti dalle fibre di rinforzo di compositi rinforzati con fibre di vetro. La ricostruzione dell'immagine tridimensionale ha consentito la visualizzazionedella distribuzione spaziale delle fibre all'interno della matrice polimerica anche nel caso di fibre di piccole dimensioni (diametro medio di 10 micrometri. E' stato quindi possibile misurare le differenze nell'orientamento delle fibre nei differenti strati di un campione utilizzando metodi basati sulla valutazione del Mean Intercept Length (MIL e del fabric tensor. La tecnica descritta è stata applicata a un campione di poliammide 6 rinforzato con il 30% di fibre corte di vetro ricavato da lastra.

  4. Ricerche sugli enterobatteri nelle feci di Chirotteri

    OpenAIRE

    Dino Scaravelli; Paola Massi; Giovanni B. Tosi; Massimo Bertozzi

    2003-01-01

    Dal 2002, grazie al protocollo stipulato tra il gruppo di studio chirotteri del Museo di Onferno e l?Istituto Zooprofilattico Sperimentale della Lombardia e dell?Emilia, sezione di Forlì, si è iniziato un progetto di studio sulla enteroflora di varie specie di Chirotteri. I campioni fecali o più raramente da carcassa, sono stati procssati mediante sistemi standardizzati e tipizzati mediante Enterotubes, creando anche una collezione di riferimento. Le ricerche ad oggi hanno interessato campion...

  5. Ricerca di contaminanti in tessuti animali e in alimenti destinati all'uomo.

    OpenAIRE

    Sori, Francesca

    2014-01-01

    La contaminazione chimica rappresenta uno dei rischi principali per la sicurezza alimentare e può arrecare anche gravi danni alla salute umana. Rientrano in questa tesi di dottorato tre famiglie di contaminanti: Micotossine, Metalli e Insetticidi. La ricerca di aflatossina B1 è stata effettuata su 90 confezioni di farina, sia biologici sia convenzionali. La presenza della micotossina è stata rilevata solo nelle farine di mais. Solo un campione di produzione convenzionale ha superato il li...

  6. L'abitato dell'Età del Bronzo di S. Giovanni in Triario (Bologna

    Directory of Open Access Journals (Sweden)

    Giacomo Vinci

    2012-12-01

    Full Text Available Questo articolo riassume i risultati delle ricerche condotte a S. Giovanni in Triario nella pianura a Nord-Est di Bologna, mettendo insieme numerosi dati di natura diversa provenienti dalla documentazione di vecchi scavi di emergenza, l'analisi aerofotografica e dalle recenti indagini condotte sul sito costituite da ricognizioni di superficie e carotaggi manuali. Grazie alle nuove indagini condotte sul deposito stratigrafico e allo studio di un consistente campione di manufatti rendono ora possibile delineare gli elementi cronologici.

  7. L'abitato dell'Età del Bronzo di S. Giovanni in Triario (Bologna)

    OpenAIRE

    Giacomo Vinci

    2012-01-01

    Questo articolo riassume i risultati delle ricerche condotte a S. Giovanni in Triario nella pianura a Nord-Est di Bologna, mettendo insieme numerosi dati di natura diversa provenienti dalla documentazione di vecchi scavi di emergenza, l'analisi aerofotografica e dalle recenti indagini condotte sul sito costituite da ricognizioni di superficie e carotaggi manuali. Grazie alle nuove indagini condotte sul deposito stratigrafico e allo studio di un consistente campione di manufatti rendono ora po...

  8. Purchasing Drivers of Professional Wine Buyers. The Role of Denominations of Origin in the Buynig Decision of Italian Upscale Restaurants

    OpenAIRE

    V. Zampi; M. Faraoni; di Folco, E.

    2008-01-01

    Attraverso un'ampia ricerca empirica condotta a livello italiano su un campione statistico di acquirenti professionali nel settore della ristorazione qualificata, è stato definito il posizionamento relativo di alcune delle principali denominazioni di origine di vini italiani cercando anche di identificare quali siano le determinanti del posizionamento di un brand territoriale. abstract: This paper investigate the perceived value of territorial wine brands (appellations) in the specialize...

  9. Central radio galaxies in groups: cavities, bubbles and the history of AGN heating

    Science.gov (United States)

    Giacintucci, S.; Venturi, T.; Raychaudhury, S.; Vrtilek, J.

    2008-10-01

    E' noto che le regioni centrali degli ammassi e gruppi di galassie costituiscono un ambiente in cui gas caldo e plasma radioemittente proveniente dalle galassie dominanti interagiscono tra loro. In particolare, si pensa che la radioemissione dell'AGN centrale ed i suoi possibili cicli di attivita', siano strettamente legati alla presenza di cavita' e "bubbles" nel gas intergalattico. Si presentera' lo status di un progetto osservativo effettuato con il Giant Metrewave Radio Telescope (GMRT, India) su di un campione di 18 gruppi di galassie, osservati a tre frequenze radio (235 MHz, 325 MHz e 610 MHz). Lo studio della morfologia radio degli AGN centrali e la relativa analisi spettrale permettono di ottenere stime sull'eta' di questi oggetti, e sulla loro energia totale, che a loro volta sono in relazione con le proprieta' X dei gruppi stessi. Per tutti gli oggetti del campione sono disponibili osservazioni Chandra di proprieta'. Per due oggetti del campione, 4C+24.36 (al centro di AWM04) e NGC741 (al centro di RSOG17) verra' presentato uno studio dettagliato.

  10. Rhizosphere effect of different aquatic plants on phosphorus depletion

    Institute of Scientific and Technical Information of China (English)

    Zhenyu WANG; Shengfang WEN; Baoshan XING; Dongmei GAO; Fengmin LI

    2008-01-01

    A series of pot experiments with Alternanthera philoxeroides, Typha latifolia, Sagittaria sagittifolia and Phragmites communis were conducted to assess the phos-phorus depletion effect in the rhizosphere. The ratio of root to shoot, root morphology, phosphorus uptake efficiency and phosphorus utilization efficiency were analyzed. An obvious variation in phosphorus concentrations between the rhizosphere soil and non-rhizosphere soil was observed. The water-soluble P contents in the rhizosphere soil of A. philoxeroides, T. latifolia, S. sagittifolia and P. communis were reduced by 81%, 42%, 18% and 16%, respectively, compared with that in the non-rhizosphere soil. A. philox-eroides had the highest phosphorus uptake efficiency (1.32 mg/m), while T. latifolia achieved the effective phos-phorus depletion by the strong rooting system and the high phosphorus uptake efficiency (0.52 mg/m). T. latifolia not only used phosphorus to produce biomass economically, but also adjusted carbon allocation to the roots to explore the soil for more available phosphorus. A. philoxeroides and T. latifolia were more effective in depleting phosphorus in the rhizosphere than S. sagittifolia and P. communis.

  11. Uptake of uranium by plants growing on and around uranium mill tailings pond at Jaduguda, India

    International Nuclear Information System (INIS)

    A field study was conducted in an area where uranium mill tailings are discharged in the form of slurry (mixture of fine sand and effluent). The fine tailings sand is retained there and effluent is decanted for further treatment. Over the years, certain plant species like Typha latifolia, Saccharum spontanium, Ipomoea carnia etc. have covered the major portion of the tailings pond. Concentration and concentration ratio of uranium in different organs of these plants were evaluated. Concentration of uranium in Typha latifolia plant from tailings pond and the CR was found to have inverse relationship with substrate uranium content. Correlation coefficient between CR(R) and soil, CR(St) and soil and CR(L) and soil in Typha latifolia was -0.80, -0.90 and -0.86 respectively. (author)

  12. THE ABILITY OF LEAVES AND RHIZOMES OF AQUATIC PLANTS TO ACCUMULATE MACRO- AND MICRONUTRIENTS

    Directory of Open Access Journals (Sweden)

    Agnieszka Edyta Parzych

    2015-06-01

    Full Text Available The samples of macrophytes and bottom sediments originated from the littoral zone of the Słupia River were collected in summer 2013. The aim of this study was to compare the properties of the accumulation of leaves and rhizomes of Glyceria maxima, Phragmites australis, Typha latifolia and Phalaris arundinacea for macro- and micronutrients. The largest quantities of macroelements were found in the leaves of the examined species, and microelements dominated the rhizomes of most examined macrophytes except for Mn in P.australis and T.latifolia. The obtained results show that N and K dominated in the leaves of P.arundinacea, P and Mg in the leaves of P.australis, and Ca in the leaves of G.maxima. The largest quantities of N, P and K were cumulated in the rhizomes of P.arundinacea, while Mg and Ca in the rhizome of T.latifolia. The leaves of aquatic plants accumulated from 1354.9 mmolc·kg-1 (T.latifolia to 1844.0 mmolc·kg-1 (P.arundinacea, and rhizomes from 985.8 mmolc·kg-1 (G.maxima to 1335.2 mmolc·kg-1 (P.arundinacea of all the analyzed components. In these species of macrophytes lower accumulated value of the sum of macro- and microelements were found in the rhizomes. The share of nitrogen was 42.4–59.8% of this amount, phosphorus 4.3–8.6%, potassium 22.8–35.1%, calcium from 2,6% to 12.4%, magnesium 3.0–7.5%, and heavy metals were from 0.6% (G.maxima to 1.2% (T.latifolia in leaves and from 2.2% (T.latifolia to 8.7% (G.maxima in rhizomes.

  13. The Chromosome Number of Psammosilene tunicoides Endemic to China%中国特有植物金铁锁的细胞学研究

    Institute of Scientific and Technical Information of China (English)

    潘跃芝; 杨志云; 龚洵

    2004-01-01

    In this paper, Psammosilene tunicoides, an endemic species to China, was cytologically studied for the first time. The morphology of the nuclei at resting stage was categorized to be simple chromocentre type. The morphology of mitofic-pmphase chromosomes was categorized to be the interstitial type.28 chromosomes were observed at the mitotic metaphase, and 14 bivalent chromosomes were observed at diakinesis. So, the basic chromosome number was confirmed to be x = 14. Psomzr~silene tunicoides is different from Silene rubicunda in the basic chromosome number and the morphology of the nuclei at resting stage and mitotic-prophase chromosomes, because Sgene rub/cund has the basic chromosome number of x= 10 and 12, and its nuclei at resting stage and mitotic-prophase chromosomes is sparsely diffuse type and continuous type respectivrly.

  14. Palatability of weeds from different European origins to the slugs Deroceras reticulatum Müller and Arion lusitanicus Mabille

    Science.gov (United States)

    Keller, Michael; Kollmann, Johannes; Edwards, Peter J.

    1999-04-01

    As part of a study on the significance of seed provenances in schemes to enhance biodiversity in agricultural habitats, juvenile plants of Cichorium intybus, Daucus carota, Leucanthemum vulgare and Silene alba of different European origins were exposed to grazing by two slug species, Deroceras reticulatum and Arion lusitanicus. Living plants were offered in trays, either in a glasshouse ( Deroceras) or outdoors ( Arion). The amount of herbivory was origin-dependent, with higher losses for all four species from German and Hungarian provenances compared with English and Swiss plants. The main trend was similar for both slug species except in the case of Daucus, and there was a significant 'origin × plant species' interaction. We found strong correlations between provenance-specific herbivory and certain climatic characteristics of the corresponding regions, i.e. winter minimum temperatures, and dryness in spring and late summer, which are crucial for the development of slugs. The results can be interpreted in terms of a SW-NE European climatic gradient and may be a consequence of differences in the need for plant defences against herbivory by slugs. Additionally, the data on palatability were compared with susceptibility towards two parasites which occurred in a field experiment, a leaf miner on Leucanthemum vulgare and a rust fungus on Silene alba. While specific leaf mining frequencies on Leucanthemum contrasted with the palatability of the different provenances to slugs, the rust infection on Silene was low on local and German plants, and higher on the more distant provenances from England and Hungary.

  15. Infrared Spectra and Density Functional Calculations for Singlet CH2═SiX2 and Triplet HC-SiX3 and XC-SiX3 Intermediates in Reactions of Laser-Ablated Silicon Atoms with Di-, Tri-, and Tetrahalomethanes.

    Science.gov (United States)

    Cho, Han-Gook; Andrews, Lester

    2016-03-21

    Reactions of laser-ablated silicon atoms with di-, tri-, and tetrahalomethanes in excess argon were investigated, and the products were identified from the matrix infrared spectra, isotopic shifts, and density functional theory energy, bond length, and frequency calculations. Dihalomethanes produce planar singlet silenes (CH2═SiX2), and tri- and tetrahalomethanes form triplet halosilyl carbenes (HC-SiX3 and XC-SiX3). The Si-bearing molecules identified are the most stable, lowest-energy product in the reaction systems. While the C-Si bond in the silene is a true double bond, the C-Si bond in the carbene is a shortened single bond enhanced by hyperconjugation of the two unpaired electrons on C to σ*(Si-X) orbitals, which contributes stabilization through a small amount of π-bonding and reduction of the HCSi or XCSi angles. The C-Si bond lengths in these carbenes (1.782 Å for HC-SiF3) are between the single-bond length in the unobserved first insertion intermediate (1.975 Å for CHF2-SiF) and the double-bond length in the silene (1.704 Å for CHF═SiF2). The silicon s(2)p(2) and titanium s(2)d(2) electron configurations produce similar primary products, but the methylidyne with Ti has a bond to carbon stronger than that of the halosilyl carbene.

  16. Sull'utilità e il danno della letteratura per la vita

    Directory of Open Access Journals (Sweden)

    Federico Bertoni

    2013-05-01

    Full Text Available Il saggio prende spunto da due romanzi-campione, L'educazione sentimentale di Flaubert e Lolita di Nabokov, per sviluppare una riflessione sulle trame del desiderio e dell'amore nella modernità borghese. Il quadro teorico di riferimento è il rapporto tra letteratura ed esperienza, tra la percezione del mondo e la sua trascrizione nel linguaggio, con riferimento specifico a una delle esperienze fondamentali della vita umana, la passione amorosa. Il saggio intreccia riflessioni di tipo tematico con un'analisi minuziosa degli aspetti stilistico-formali.

  17. Subsurface microbial ecology. Epi fluorescence direct counts; Ecologia microbica del sottosuolo: metodo di conta diretta in epifluorescenza

    Energy Technology Data Exchange (ETDEWEB)

    Barra Caracciolo, A.; Silvestri, C.; Creo, C.; Izzo, G. [ENEA, Centro Ricerche Casaccia, Rome (Italy). Dipt. Ambiente

    1998-07-01

    To the aim of recognize the importance of microorganisms in affecting or even determining the fate of xenobiotics in the subsurface environment evaluating bacteria concentration in a subsurface ecosystem, the report discusses a soil sample treatment method which has been developed for epi fluorescence direct counting with DAPI. [Italian] Lo studio discute un metodo di trattamento del campione per la conta diretta in epifluorescenza con un marcatore selettivo per il DNA, il DAPI, al fine di quantificare la concentrazione batterica del sottosuolo e studiare il ruolo dei microrganismi nella biodegradazione delle molecole esogene, ancora poco indagato.

  18. Analisi sui pigmenti di ceramica neolitica tramite tecniche Raman e LIBS

    Directory of Open Access Journals (Sweden)

    Cristina Fabbri

    2007-04-01

    Full Text Available I dati presentati in questo breve contributo provengono da uno studio condotto in collaborazione dal Dipartimento di Scienze Archeologiche dell’Università di Pisa e dall’Istituto per i Processi Chimico-Fisici di Fisica molecolare del CNR di Pisa. Le metodologie applicate sono basate sull’analisi integrata delle tecniche spettroscopiche Raman e LIBS già ampiamente utilizzate nel campo dei Beni Culturali; i metodi sono infatti essenzialmente non distruttivi, non richiedono di un pre trattamento del campione e l’acquisizione delle misure è immediata.

  19. Aspetti dell'ecologia della lepre sarda (Lepus capensis mediterraneus) in aree della Sardegna meridionale. Some aspects of cape hare Lepus capensis mediterraneus ecology in Southern Sardinia

    OpenAIRE

    Murgia, Carlo; Murgia, Andrea; Deiana, Anna Maria

    2003-01-01

    Nonostante la Lepre sarda (Lepus capensis mediterraneus) sia una specie soggetta a prelievo, poco o nulla si conosce circa la sua ecologia, ed in particolare in Provincia di Cagliari nessuna ricerca era stata fino ad ora condotta. Scopo di questa indagine è stato quello di individuare i valori di densità, dinamica di popolazione e selezione dell'habitat. Sono stati effettuati dei conteggi notturni con l'uso di proiettori alogeni su percorsi campione negli anni 1994-95, 1995-96 e 1997-98; n...

  20. Lavoro di cura, famiglie e futuro: riflessioni per uno strumento di ricerca.

    Directory of Open Access Journals (Sweden)

    Felice Carugati

    2007-07-01

    Full Text Available Il presente contributo riguarda le riflessioni teoriche che hanno portato alla costruzione di uno strumento di ricerca sul tema delle cure educative che le madri pensano ed agiscono nella vita quotidiana nei confronti dei figli. Utilizzando una prospettiva che studia i contesti sociali come unità d’analisi a più livelli, dall’individuale al collettivo (Bronfenbrenner, 1979, dalle azioni alle rappresentazioni (Doise, 1982, integrando in questa prospettiva anche lo studio delle Rappresentazioni Sociali (Mugny, Carugati, 1985, vengono descritti i principali contenuti di un questionario somministrato ad un campione di madri con figli di età tra i 5 e gli 11 anni.

  1. Effects of plant species on soil microbial processes and CH4 emission from constructed wetlands

    International Nuclear Information System (INIS)

    Methane (CH4) emission from constructed wetland has raised environmental concern. This study evaluated the influence of mono and polyculture constructed wetland and seasonal variation on CH4 fluxes. Methane emission data showed large temporal variation ranging from 0 to 249.29 mg CH4 m−2 h−1. Results indicated that the highest CH4 flux was obtained in the polyculture system, planted with Phragmites australis, Zizania latifolia and Typha latifolia, reflecting polyculture system could stimulate CH4 emission. FISH analysis showed the higher amount of methanotrophs in the profile of Z. latifolia in both mono and polyculture systems. The highest methanogens amount and relatively lower methanotrophs amount in the profile of polyculture system were obtained. The results support the characteristics of CH4 fluxes. The polyculture constructed wetland has the higher potential of global warming. -- Highlights: ► The polyculture constructed wetland has the higher contribution to CH4 emission. ► The CH4–C conversion ranged from 0 to 3.7%. ► The Z. latifolia played important roles in methanotrophs growth and CH4 consumption. ► Major influence of T-N, TOC and plant cover on CH4 emission was obtained. -- The polyculture constructed wetland has the higher contribution to global warming

  2. A synopsis of the Malesian species of Kibara (Monimiaceae)

    NARCIS (Netherlands)

    Philipson, W.R.

    1985-01-01

    Thirty-nine species are recognized of which twenty-four are described as new ( K. bullata, K. carrii, K. chimbuensis, K. ferox, K. flagelliformis, K. fragrans, K. fugax, K. hartleyi, K. karengana, K. katikii, K. kostermansii, K. latifolia, K. leachii, K. macrantha, K. nitens, K. novobritanica, K. ob

  3. Potentiometric Demonstration of Metal Biosorption by Nonliving Plants

    Science.gov (United States)

    Velazquez-Jimenez, Litza Halla; Torres-Rodriguez, Luz Maria; Garcia-de la Cruz, Ramon Fernando; Montes-Rojas, Antonio; Lopez-Arteaga, Rafael Eduardo

    2010-01-01

    An electrochemical experiment is presented to illustrate biosorption to second-year and upper-division undergraduate students. The extraction of Cd[superscript 2+], Pb[superscript 2+], and Cu[superscript 2+] by nonliving "Typha latifolia" (cattail) roots can be monitored in real time by potentiometry determinations. The open circuit potential…

  4. Bioactivity of non-edible oil seed extracts and purified extracts against Helicoverpa armigera (Hubner).

    Science.gov (United States)

    Pawar, Pushpa; Joseph, Mary; Tungikar, Vijay; Joshi, Swati

    2004-01-01

    Extracts and purified extracts of seeds of two plant species, Madhuca latifolia and Calophyllum inophyllum when evaluated against the 2nd instar larvae of Helicoverpa armigera reared on synthetic diet, exhibited high larval mortality, prolongation of developmental period, morphological deformities and highly significant reduction in adult emergence. The reduction in larval weights in the treatments was also highly significant. PMID:15274488

  5. GenBank blastn search result: AK059600 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK059600 001-030-E05 AY151361.1 Oryza latifolia clone 7 internal transcribed spacer... 1, 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence.|PLN PLN 2e-80 Plus Plus ...

  6. GenBank blastn search result: AK059600 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK059600 001-030-E05 AF513561.1 Oryza latifolia strain IRRI 100167 clone 9 internal transcribed spacer... 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence.|PLN PLN 2e-80 Plus Plus ...

  7. GenBank blastn search result: AK059600 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK059600 001-030-E05 AF513587.1 Oryza latifolia strain IRRI 100914 clone 11 internal transcribed spacer... 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence.|PLN PLN 2e-80 Plus Plus ...

  8. GenBank blastn search result: AK059600 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK059600 001-030-E05 AF513581.1 Oryza latifolia strain IRRI 100914 clone 5 internal transcribed spacer... 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence.|PLN PLN 2e-80 Plus Plus ...

  9. GenBank blastn search result: AK059600 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK059600 001-030-E05 AF513591.1 Oryza latifolia strain IRRI 105145 clone 3 internal transcribed spacer... 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence.|PLN PLN 7e-83 Plus Plus ...

  10. GenBank blastn search result: AK059600 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK059600 001-030-E05 AF513568.1 Oryza latifolia strain IRRI 100171 clone 4 internal transcribed spacer... 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence.|PLN PLN 2e-80 Plus Plus ...

  11. GenBank blastn search result: AK107169 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK107169 002-124-F12 AF513597.1 Oryza latifolia strain IRRI 105145 clone 9 internal transcribed spacer... 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence.|PLN PLN 8e-83 Plus Plus ...

  12. GenBank blastn search result: AK059600 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK059600 001-030-E05 AF513595.1 Oryza latifolia strain IRRI 105145 clone 7 internal transcribed spacer... 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence.|PLN PLN 2e-80 Plus Plus ...

  13. A model on the effect of temperature and moisture on pollen longevity in air-dry storage environments

    NARCIS (Netherlands)

    Hong, T.D.; Ellis, R.H.; Buitink, J.; Walters, J.; Hoekstra, F.A.; Crane, J.

    1999-01-01

    Data on the survival of pollen ofTypha latifoliaL. stored for up to 261 d over seven different saturated salt solutions (providing 0.5 to 66% relative humidity) and six different constant temperatures (from −5 to +45 °C) were analysed to quantify the effect of air-dry storage environment on pollen l

  14. Notities over Nederlandse zeewieren I

    OpenAIRE

    Mol, I.

    1984-01-01

    Four species considered to be newly found drifting ashore are described and illustrated: Sargassum natans (L.) Børg., Champia parvula (C. Ag.) Harv., Crouania attenuata (C. Ag.) J. Ag. and Callophyllis laciniata (Huds.) Kütz. In addition some notes are made on new records of Pogotrichum filiforme Reinke, Leathesia difformis (L.) Aresch. and Punctaria latifolia Grev.

  15. Disponibilidade e valor nutritivo de forragem de leguminosas nativas (Adesmia DC. e exóticas (Lotus L. Availability and nutritive value of the wild leguminous (Adesmia DC. and exotics (Lotus L.

    Directory of Open Access Journals (Sweden)

    Simone Meredith Scheffer-Basso

    2001-06-01

    Full Text Available O gênero Adesmia DC. possui 17 espécies nativas no Brasil, distribuídas nos Estados do Sul, cuja importância está vinculada a sua adaptação ao solo e clima regionais, além de ser de crescimento hibernal (temperadas. Este trabalho teve o objetivo comparar o padrão de acúmulo de matéria seca (MS e valor nutritivo de forragem de A. latifolia, A. punctata e A. tristis, tendo como padrão Lotus corniculatus (cornichão e L. uliginosus. O ensaio foi realizado em casa de vegetação durante 210 dias (4000 graus-dia. A disponibilidade de forragem (DF foi similar entre A. latifolia (276 g MS/m² e cornichão (275 g MS/m² e entre A. tristis (201g MS/m² e L. uliginosus (192 g MS/m², sendo que A. punctata apresentou a menor DF (155 g MS/m². A. latifolia caracterizou-se pela maior precocidade na DF, devido ao crescimento mais rápido em relação às demais espécies, sugerindo seu potencial para utilização durante a estação fria. Em relação às análises de qualidade, o teor de proteína bruta (PB nas folhas de A. latifolia foi de até 21,6% e a DIVMO atingiu 72,3%. Os maiores conteúdos de PB e DIVMO foram encontrados nas folhas de cornichão, 30,3 e 75,8%, respectivamente. A. tristis apresentou DIVMO muito baixa nos caules (34,9 a 44,7%, o que poderia limitar seu consumo por bovinos. Concluiu-se que, entre as espécies de Adesmia estudadas, A. latifolia detém o maior potencial forrageiro, sugerindo a continuidade de estudos com a espécie.The genus Adesmia DC. has 17 species native to Brazil, distributed in the Southern states, whose importance is linked to its adaptation to the soils and climatic conditions of the region, besides being an active winter-growing species (temperate. This work aimed to compare the patterns of dry matter (DM accumulation and nutritive value of A. latifolia, A. punctata and A. tristis, using Lotus corniculatus (birdsfoot trefoil and L. uliginosus (big trefoil, as checks. The experiment was carried out in

  16. Relationship between carbon and water economies and drought-vulnerability in two coexistent iso- and anisohydric species.

    Science.gov (United States)

    Garcia-Forner, Núria; Biel, Carmen; Savé, Robert; Martínez-Vilalta, Jordi

    2016-04-01

    Stomata control carbon and water fluxes between leaves and the atmosphere. Isohydry (i.e., strong regulation of leaf water potential, Ψl) is commonly linked to 'early' stomatal closure under drought, which in turn is believed to imply lower hydraulic risk at the expense of reduced carbon assimilation. Hence, the iso/anisohydric classification has been widely used to assess drought-resistance and mortality mechanisms across species, but the underlying assumptions have been rarely tested. These include a direct correspondence between iso/anisohydric Ψl regulation and stomatal behavior across species, and similar vulnerability to xylem embolism in iso- and anisohydric species. Our objective is to assess the physiological mechanisms underlying drought-resistance differences under controlled, experimental conditions between two coexistent Mediterranean forest species with contrasted drought-vulnerability in the field: the resistant Phillyrea latifolia (anisohydric) and vulnerable Quercus ilex (isohydric). We hypothesize that lower Ψl in P. latifolia will not necessarily be associated with narrower hydraulic safety margins or longer periods of positive gas exchange under drought. Isohydric behavior was confirmed in Q. ilex, but did not imply lower hydraulic impairment, due to lower resistance to xylem embolism in this species. We found similar temporal patterns of stomatal conductance and assimilation between species. If anything, the anisohydric P. latifolia tended to show lower assimilation rates than Q. ilex under extreme drought. The fact that P. latifolia was as carbon-constrained as Q. ilex was also indicated by similar growth rates and carbon reserves dynamics in both species. Despite similarities in carbon management between species, after two years with no water supply P. latifolia mortality was less than half of Q. ilex mortality by this time. Our study warns against making direct connections between Ψl regulation, stomatal behavior and the mechanisms of

  17. Caratteristiche dei segni di marcatura territoriale nel capriolo, Capreolus capreolus L., 1758

    Directory of Open Access Journals (Sweden)

    Rocco Sorino

    2003-10-01

    Full Text Available I maschi maturi di capriolo sono territoriali dagli inizi della primavera (aprile fino all?epoca degli amori, tardo agosto ? inizio settembre. Durante il periodo territoriale, i maschi eseguono due tipi di marcatura olfattiva a significato territoriale: le raspate e i fregoni. Le prime sono effettuate con le ghiandole site tra le dita delle zampe anteriori, i secondi con le ghiandole poste alla base dei palchi. Nel presente contributo si riportano alcuni risultati preliminari sull?abbondanza relativa dei due tipi di marcatura in due habitat alpini con differente copertura vegetazionale, per stabilire se questa influenzi la scelta del tipo di marcatura. L?area di studio è situata nella Val Chisone (Alpi nord ? occidentali ed è compresa tra i 1800 e i 1900 metri di quota con una superficie di 238,5 ha. L?area è caratterizzata da un pascolo di 96,5 ha (pari al 40,5% dell?area e da un lariceto di 142 ha (pari al 59,5% dell?area. Lo studio è stato effettuato nel periodo estivo nell?arco del triennio 2001-2003. I segni territoriali sono stati rilevati in aree di forma quadrata con lati di 15 metri (225 m² campionate con un metodo di randomizzazione. In ogni area campione si è valutato il numero, le dimensioni e la densità dei segni di marcatura nonché il tipo di stratificazione vegetazionale (arborea, arbustiva, erbacea, le classi percentuali di copertura, il numero di piante marcate e quelle potenzialmente marcabili. Per valutare l?esistenza di correlazione tra i segni di marcatura e i diversi parametri ambientali misurati si sono utilizzati dei test di Spearman. Sono stati rilevati 52 fregoni (x = 3,71; SE = 0,90 e 24 raspate (x = 1,71; SE = 0,57. Il 68% dei fregoni è stato rilevato in aree campione con una densità di alberelli inferiore a 3,1 alberelli/10 m², mentre il 100% delle raspate è stato rinvenuto in aree campione con una densità di alberelli inferiore a 2,2 alberelli/10 m². In ogni area campione il numero di fregoni

  18. О НОВЫХ И НАИБОЛЕЕ РЕДКИХ ВИДАХ ТАМБОВСКОЙФЛОРЫ. СООБЩЕНИЕ 4

    OpenAIRE

    Соколов, Александр; Соколова, Людмила

    2014-01-01

    В работе сообщаются сведения о новых (Astragalus pubiflorus (Pall.)) DC., Veronica persica Poir., Dipsacus strigosus Willd. ex Roem. et Schult.) и наиболее редких (Ephedra distachya L., Stipa lessingiana Trin. et Rupr., S. pulcherrima C. Koch, Rhynchospora alba (L.) Vahl, Carex humilis Leysser, Veratrum nigrum L., Anthericum ramosum L., Lilium martagon L., Ceratocarpus arenarius L., Arenaria procera Spreng., Silene sibirica (L.) Pers., Actaea spicata L., Aconitum anthora L., Clematis integrif...

  19. Al lelopathic Effect of Al ien I nvasive Borreria latifol ia on Solanaceae Crops%阔叶丰花草对茄科作物的化感效应

    Institute of Scientific and Technical Information of China (English)

    陶文琴; 许镇健; 黄丽宜; 缪绅裕; 杨礼香; 王厚麟; 陈健辉

    2014-01-01

    The germination rate,root and stem length of tomato,eggplant and pepper seeds treated with different concentration of B.latifolia water extract were determined by seed germination and biological determination methods to study the effects of alien invasive B.latifolia on seed germination and growth of seedlings of Solanaceae crops.The results showed that:1 )The germination rate index of tomato and eggplant decreases with increase of B.latifolia water extract concentration significantly;2) The germination rate of tomato seeds is lower than CK but the seed germination of different treatments is above 93.33%;3 )The seed germination rate of eggplant and pepper decreases with increase of B. latifolia water extract concentration and is above 70% and above 66.67% after 14 d respectively;4)The root length of tomato and eggplant treated with different concentration of B.latifolia water extract is lower than CK significantly,except for pepper under the higher concentration;5)The stem length of tomato seedlings after 7d is shorter than CK but the stem length of tomato seedlings after 14d is higher than CK.The integrated allelopathic effect of B.latifolia on tomato is the inhibiting effect.The integrated allelopathic effect of B.latifolia with lower concentration and higher concentration is the promoting effect and inhibiting effect separately.Pepper shows the certain adaptability to B.latifolia.%为探明入侵植物阔叶丰花草对茄科作物种子萌发和幼苗生长的影响,采用种子萌发与生物测定法,测定比较了阔叶丰花草不同浓度水提液处理番茄、茄子和辣椒种子的萌发率、幼苗的根长及茎长。结果表明:番茄和茄子的发芽速度指数随处理浓度上升而显著降低;番茄种子的萌发率略低于对照,均在93.33%以上;茄子和辣椒种子的萌发率均随水提液浓度的上升而降低,14 d 时分别在70%以上和66.67%以上;各浓度水提液番茄和茄子的根长显著

  20. [Treatment of marine-aquaculture effluent by the multi-soil-layer (MSL) system and subsurface flow constructed wetland].

    Science.gov (United States)

    Song, Ying; Huang, Yu-ting; Ge, Chuan; Zhang, Hao; Chen, Xin; Zhang, Zhi-jianz; Luo, An-cheng

    2014-09-01

    To evaluate the feasibility of using multi-soil-layer (MSL) system and subsurface flow constructed wetland to treat the wastewater of marine cultured Penaeus vannamei and to determine the suitable process for the local aquaculture wastewater pollution characteristics. In this study, MSL system and four constructed wetland systems with Spartina anglica, Phragmites australis, Typha latifolia and unplanted system were evaluated for their potentials of pollutants removal capacity. The results showed the average removal rates of chemical oxygen demand (COD), total phosphorus (TP), total nitrogen (TN), ammonia nitrogen (NH(4)+ -N) and nitrate (NO-(3) -N) by MSL system were 80. 38% ± 2. 14% , 68. 14% ± 3.51% , 40.79% ± 3. 10% , 42. 68% ± 2.90% and 54. 19% ± 5. 15% , respectively. Additionally, the ability of pollutants removal of other four wetland systems decreased in the order: Spartina anglica, Phragmites australis, Typha latifolia and unplanted system. PMID:25518662

  1. Meiotic behavior of Adesmia DC. (Leguminosae-Faboideae species native to Rio Grande do Sul, Brazil

    Directory of Open Access Journals (Sweden)

    Coelho Liliana Gressler May

    1998-01-01

    Full Text Available Meiotic behavior in Adesmia DC. is described for the first time. The study encompassed twelve populations of seven Adesmia DC. species native to Rio Grande do Sul, Brazil. Populations with 2n = 2x = 20 are A. securigerifolia 9615, A. riograndensis 9590 (subnudae, A. latifolia 1568, 1775, 15025, A. bicolor JB-UFSM, A. incana var. incana 9636, 10288, A. punctata var. hilariana 6885, 10812, and A. tristis 10757. A. incana var. incana 9637 is a tetraploid with 2n = 4x = 40. The material was stained with 1% acetic orcein. The meiotic behavior of the populations studied was considered normal. The meiotic index (MI and the estimates of pollen grain viability were above 95%, except for A. latifolia 1568 (MI = 89%. The present data indicate that these plants are meiotically stable and potentially fertile, apparently with no problems for use in programs of selection, crossing and viable seed production.

  2. Comportamenti sessuali e rischio di AIDS in studenti universitari

    Directory of Open Access Journals (Sweden)

    M.L. Sammarco

    2003-05-01

    Full Text Available

    Obiettivi: la trasmissione eterosessuale di HIV sta acquisendo una sempre maggiore importanza. Obiettivo del presente studio è stato, pertanto, quello di valutare in un campione di studenti universitari le caratteristiche dei comportamenti sessuali a rischio.

    Metodi: nell’ambito di uno studio più ampio, è stato distribuito ad un campione di studenti dell’Università del Molise (Campobasso un questionario anonimo che comprendeva anche una sezione sui comportamenti sessuali. Il campione era composto da 430 studenti (maschi = 35,8%; l’età media era di 23 anni.

    Risultati: il 72.6% ha riferito rapporti sessuali completi (83,6%, femmine = 66,5%; p‹0,005, test del chiquadrato; più spesso i rapporti sono senza l’uso del preservativo (55,9%; maschi = 55,0%, femmine = 56,6%. Una discreta percentuale (18,5%; maschi = 29,9%, femmine = 10,5%, p‹0,005 ha avuto rapporti occasionali. Il 51,8% degli studenti sessualmente attivi ha avuto rapporti sessuali dopo aver bevuto alcolici (maschi = 65,9%; femmine = 41,9%; p‹0,05; tra questi il 54,6% (maschi = 50,0%, femmine = 59,5% non utilizza il preservativo. Una percentuale più contenuta (16,3%; maschi = 23,6%, femmine = 11,1%, p‹0,05 ha avuto rapporti sessuali dopo l’uso di droghe (soprattutto cannabis e cocaina; tra questi il 55,3% (maschi = 56,7%, femmine = 52,9% non utilizza il preservativo. Il 6,7% dei maschi ha avuto almeno un’esperienza omosessuale con rapporti anali, di cui una metà non ha utilizzato il preservativo nell’ultimo rapporto.

    Conclusioni: i maschi sono sessualmente più attivi e hanno comportamenti più a rischio, tuttavia non ci sono differenze significative per quanto riguarda l’uso del preservativo. Nel nostro paese il futuro dell’epidemia di AIDS dipenderà dai comportamenti sessuali della popolazione. A tal fine le strategie di prevenzione devono mirare ad aumentare la

  3. Characterizing the physical and genetic structure of the lodgepole pine × jack pine hybrid zone: mosaic structure and differential introgression

    OpenAIRE

    Cullingham, Catherine I; James, Patrick M. A.; Cooke, Janice E.K.; Coltman, David W

    2012-01-01

    Understanding the physical and genetic structure of hybrid zones can illuminate factors affecting their formation and stability. In north-central Alberta, lodgepole pine (Pinus contorta Dougl. ex Loud. var. latifolia) and jack pine (Pinus banksiana Lamb) form a complex and poorly defined hybrid zone. Better knowledge of this zone is relevant, given the recent host expansion of mountain pine beetle into jack pine. We characterized the zone by genotyping 1998 lodgepole, jack pine, and hybrids f...

  4. Large-scale asymmetric introgression of cytoplasmic DNA reveals Holocene range displacement in a North American boreal pine complex

    OpenAIRE

    Godbout, Julie; Yeh, Francis C.; Bousquet, Jean

    2012-01-01

    Jack pine (Pinus banksiana) and lodgepole pine (Pinus contorta var. latifolia) are two North American boreal hard pines that hybridize in their zone of contact in western Canada. The main objective of this study was to characterize their patterns of introgression resulting from past and recent gene flow, using cytoplasmic markers having maternal or paternal inheritance. Mitochondrial DNA (mtDNA) and chloroplast DNA (cpDNA) diversity was assessed in allopatric populations of each species and i...

  5. Characterization of Root-Associated Methanotrophs from Three Freshwater Macrophytes: Pontederia cordata, Sparganium eurycarpum, and Sagittaria latifolia†

    OpenAIRE

    Calhoun, A.; King, G. M.

    1998-01-01

    Root-associated methanotrophic bacteria were enriched from three common aquatic macrophytes: Pontederia cordata, Sparganium eurycarpum, and Sagittaria latifolia. At least seven distinct taxa belonging to groups I and II were identified and presumptively assigned to the genera Methylosinus, Methylocystis, Methylomonas, and Methylococcus. Four of these strains appeared to be novel on the basis of partial 16S ribosomal DNA sequence analysis. The root-methanotroph association did not appear to be...

  6. Rapportage onderzoek aantasting van de bast bij laanbomen

    OpenAIRE

    Lammeren, van, ACAP Andre; Ruiter, N.C.A.; Kieft, H

    2009-01-01

    In dit verslag zijn aantastingen op de stam onderzocht van Carpinus betulus ‘Frans Fontaine’, Fagus sylvatica “ Atropurpurea”, Fraxinus excelsior ‘Atlas’, Quercus palustris, Quercus robur, Sorbus latifolia “Henk Vink” en Ulmus ‘Clusius’ Daarbij is aandacht besteed aan het voorkomen en de aard en ontwikkeling van bastknobbels, baststrepen, bastscheuren, verkleuringen en het effect van epifyten zoals schimmels en korstmossen

  7. Evaluation of antidiabetic activity of plants used in Western Sudan

    Institute of Scientific and Technical Information of China (English)

    Maha; Abdulrahman; Alamin; Ahmed; Ibrahim; Yagi; Sakina; Mohamed; Yagi

    2015-01-01

    Objective: To investigate the traditional antidiabetic uses of some indigenous Sudanese plants on streptozotocin-induced diabetes rats. Methods: Diabetic rats were treated with a 400 mg/kg dose of aqueous extracts of five plant species orally for 2 h(acute) or 14 days(chronic). In acute model blood glucose levels were monitored at specific intervals. In the chronic model blood samples were collected from overnight fasted diabetic rats on day 15 to estimate blood glucose level. And the body weight, serum lipid profile and activities of liver and kidney enzymes were measured. Histopathological observations of liver sections were also studied.Results: In the case of acute treatment, aqueous extracts of Tinospora bakis(T. bakis), Nauclea latifolia(N. latifolia) and Randia nilotica(R. nilotica) at 400 mg/kg significantly lowered(P < 0.05) blood glucose levels in diabetic rats whereas, chronic treatment of diabetic rats with 400 mg/kg of T. bakis, N. latifolia, R. nilotica and Mitragyna inremis proved to have significant(P < 0.05) antihyperglycemic effect and have the capacity to correct the metabolic disturbances associated with diabetes. Histopathological studies showed that the aqueous extracts of these four plants reinforced the healing of liver. However, Striga hermonthica aqueous extract did not exert any antihyperglycemic effect to diabetic rats. Conclusions: This study demonstrated that T. bakis, N. latifolia, R. nilotica and Mitragyna inremis have therapeutic value in diabetes and related complications and thus supporting the traditional uses of these plants in Sudanese traditional medicine.

  8. IDENTIFICACIÓN DEL AGENTE CAUSAL E IMPORTANCIA DE LA GOMOSIS EN LA ZONA CITRICOLA DE HUIMANGUIILLO, TABASCO, MÉXICO

    OpenAIRE

    JA Acosta-Pérez; CF Ortiz-García; JM Zaldívar-Cruz; M Rodríguez-Cuevas; CC Bautista-Muñoz; C de la Cruz Castillo-Aguilar

    2012-01-01

    El presente estudio consistió en identicar la especie Phytophthora causante de la gomosis o podredumbre del pie en cultivos de Naranja 'Valenciana' (Citrus sinensis) y Limón 'Persa' (Citrus latifolia) en la Sabana de Huimanguillo, Tabasco. De 34 sitios muestreados sistemáticamente se lograron obtener 13 aislamientos de Phytophthora en tejidos de tallo y frutos enfermos. Para esto, se utilizaron medio selectivo agar zanahoria (zanahoria 10 %-agar Pimaricina, Rifampicina, Vancomicina y PCNB. La...

  9. Comparative analysis of A, B,C and D genomes in the genus Oryza with C0t-1 DNA of C genome

    Institute of Scientific and Technical Information of China (English)

    LAN Weizhen; QIN Rui; LI Gang; HE Guangcun

    2006-01-01

    Fluorescence in situ hybridization (FISH)was applied to somatic chromosomes preparations of Oryza officinalis Wall. (CC), O. sativa L. (AA)×O. officinalis F1 hybrid (AC), backcross progenies BC1 (AAC and ACC), O. latifolia Desv. (CCDD), O. alta Swallen (CCDD) and O. punctata Kotschy (BBCC)with a labelled probe of Cot-1 DNA from O. officinalis.In O. officinalis, the homologous chromosomes showed similar signal bands probed by C0t-1 DNA and karyotype analysis was conducted based on the band patterns. Using no blocking DNA, the probe identified the chromosomes of C genome clearly, but detected few signals on chromosomes of A genome in the F1 hybrid and two backcross progenies of BC1.It is obvious that the highly and moderately repetitive DNA sequences were considerably different between C and A genomes. The chromosomes of C genome were also discriminated from the chromosomes of D-and B-genome in the tetraploid species O. latifolia, O.alta and O. punctata by C0t-1 DNA-FISH. Comparison of the fluorescence intensity on the chromosomes of B, C and D genomes in O. latifolia, O. alta,and O. punctata indicated that the differentiations between C and D genomes are less than that between C and B genomes. The relationship between C and D genomes in O. alta is closer than that of C and D genomes in O. latifolia. This would be one of the causes for the fact that both the genomes are of the same karyotype (CCDD) but belong to different species. The above results showed that the C0t-1 DNA had a high specificity of genome and species. In this paper, the origin of allotetraploid in genus Oryza is also discussed.

  10. A Comparative Study of Bioethanol Production from Aquatic Weeds

    Directory of Open Access Journals (Sweden)

    Kodichetty Ramaiah Sunil

    2015-09-01

    Full Text Available A greatest challenge for society in the 21st century is to meet energy demand, where biomass is subjected for pre-treatment and converted into biofuel (alcohol. Aquatic weeds are potential bio resources which are easily available for biofuel production. Aquatic weeds like Alternanthera sessilis, Typha latifolia, Eichhornia crassipes, Baccopa monnieri, Ipomoea aquatica and Pistia stratiotes are estimated for carbohydrates content. Highest content of reducing sugar was observed in Alternanthera sessilis (296.8µg/ml, total sugar in Ipomoea aquatic (880.00mg/ml, starch in Alternanthera sessilis (57.13mg/ml, cellulose in Pistia stratiotes and Typha latifolia (280.00mg/ml, hemicellulose in Typha latifolia (26.85mg/ml; high cellulosic aquatic weeds were subjected to pre-treatment methods like physical, chemical and enzymatic method. Meanwhile different yeast strains from the fruits of Manilkara zapota, Cucumis melo, Musa paradisiaca, Citrullus lanatus, Punica granatum and Ananas comosus were isolated yeast of Citrullus lanatus shows highest amount of alcohol production (307µg/ml, which is inoculated to pre-treated hydrolysate, where Alternanthera sessilis and Typha latifolia shows high amount of alcohol in physical method (160.5 and 115.4µg/ml. In chemical method in acid hydrolysis it shows 387.1 and 69.63µg/ml and in alkali hydrolysis 62 and 170µg/ml, so these two weeds were taken for enzymatic method for alcohol production, on seventh day Alternanthera sessilis shows highest alcohol production (113.33µg/ml, hence among six weeds Alternanthera sessilis and the yeast of Citrullus lanatus produces more amount of alcohol than others and it also shows that enzymatic method of pre-treatment is best in hydrolysis of biomass than physical and chemical method. The study revealed the possibility of producing alcohol from locally available fruits using simple, cheap and adaptable technology with biochemically characterized yeast strains.

  11. A synopsis of the Malesian species of Kibara (Monimiaceae)

    OpenAIRE

    Philipson, W.R.

    1985-01-01

    Thirty-nine species are recognized of which twenty-four are described as new ( K. bullata, K. carrii, K. chimbuensis, K. ferox, K. flagelliformis, K. fragrans, K. fugax, K. hartleyi, K. karengana, K. katikii, K. kostermansii, K. latifolia, K. leachii, K. macrantha, K. nitens, K. novobritanica, K. oblongata, K. rosselensis, K. royenii, K. shungolensis, K. sleumeri, K. streimannii, K. sudestensis, and K. versteeghii) ). One new combination is made: K. oligocarpella (Kaneh. & Hatus.) Philipson, ...

  12. Nueva localidad para Phylodryas aestivus subcarinatus Boulenger, 1902 (Reptilia: colubridae)

    OpenAIRE

    Cordella, Carlos Enrique

    1989-01-01

    Durante una recorrida realizada el 24 de abril de 1986 en la Reserva de Fauna "Laguna La Felipa" ubicada a 12 km de la localidad de Ucacha, departamento de Juárez Celman, provincia de Córdoba, se capturó un ejemplar de Philodryas aestivus. El individuo, una hembra adulta de 1434 mm de longitud total, se encontraba en un "totoral" (Totora latifolia) cercano al agua.

  13. No evidence for niche segregation in a North American Cattail (Typha) species complex

    OpenAIRE

    McKenzie-Gopsill, Andrew; Kirk, Heather; Drunen, Wendy Van; Freeland, Joanna R.; Dorken, Marcel E.

    2012-01-01

    Interspecific hybridization can lead to a breakdown of species boundaries, and is of particular concern in cases in which one of the parental species is invasive. Cattails (Typha spp.) have increased their abundance in the Great Lakes region of North America over the past 150 years. This increase in the distribution of cattails is associated with hybridization between broad-leaved (Typha latifolia) and narrow-leaved cattails (T. angustifolia). The resulting hybrids occur predominantly as F1s,...

  14. Perfil químico y biológico de aceites esenciales de plantas aromáticas de interés agro-industrial en Castilla-La Mancha (España)

    OpenAIRE

    Santana, O.; Cabrera, R.; González-Coloma, A.; Sánchez-Vioque, R.; de los Mozos-Pascual, M.; Rodríguez-Conde, M. F.; Laserna-Ruiz, I.; Usano-Alemany, J.; Herraiz, D.

    2012-01-01

    The chemical composition and biological activities of essential oils of Salvia officinalis L., Salvia lavandulifolia Vahl., Lavandula x intermedia Emeric ex Loisel., Lavandula latifolia Medik., Lavandula angustifolia Mill. and Thymus vulgaris L. are presented. The essential oils have been analysed by Gas Chromatography Mass Spectrometry and 61 compounds were identified, 23 of whic...

  15. Assessment of post-beetle impacts on natural regeneration of Lodgepole Pine

    OpenAIRE

    Egger, Keith N; Arocena, Joselito; Green, Scott; Kennedy, Nabla; Massicotte, Hugues; Scholefield, Scott

    2009-01-01

    The ecological disturbance from wildfire (2004) on ~ 10,000 hectares of forests near the Kenny Dam presented a unique opportunity to study the natural and artificial regeneration in burned mountain pine beetle (Dendroctonus ponderosae Hopkins) infested stands in north-central British Columbia. Mountain pine beetle (MPB) has been documented as a natural disturbance agent that may precede wildfire in lodgepole pine forests (Pinus contorta var. latifolia). The objectives of this study were to i)...

  16. Activity pattern and thermal biology of a day-flying hawkmoth (Macroglossum stellatarum) under Mediterranean summer conditions

    OpenAIRE

    Herrera, Carlos M.

    1992-01-01

    1 The daily activity pattern and aspects of the thermal biology are described for the day-flying hawkmoth, Macroglossum stellatarum L. (Lepidoptera: Sphingidae), while foraging at a flowering population of Lavandula latifolia (Labiatae) under the dry-hot summer conditions of a southeastern Spanish locality. 2 The average abundance of M.stellatarum remained fairly constant from sunrise to about 17.00 hours (GMT), and a distinct peak occurred in the evening (18.00—20.00 hou...

  17. Control of the Diurnal Pattern of Methane Emission from Emergent Aquatic Macrophytes by Gas Transport Mechanisms

    Science.gov (United States)

    Whiting, Gary J.; Chanton, Jeffrey P.

    1995-01-01

    Methane emissions from Typha latifolia (L.) showed a large mid-morning transient peak associated with rising light levels. This peak was also associated with a steep decline in lacunal CH, concentrations near the stem base. This pattern contrasted sharply with emissions from Peltandra virginica (L.) that gradually rose to a peak in the mid-afternoon corresponding to elevated air temperatures. Internal CH4 concentrations within P. virginica stems did not change significantly over the diurnal period. Stomatal conductance appeared to correlate directly with light levels in both plant types and were not associated with peak CH4 emission events in either plant. These patterns are consistent with a convective throughflow and diffusive gas ventilation systems for Typha and Peltandra, respectively. Further effects of the convective throughflow in T. latifolia were evident in the elevated CH4 concentrations measured within brown leaves as contrasted to the near ambient levels measured within live green leaves. Experimental manipulation of elevated and reduced CO2 levels in the atmosphere surrounding the plants and of light/dark periods suggested that stomatal aperture has little or no control of methane emissions from T. latifolia.

  18. Evolutionary dynamics of the plastid inverted repeat: the effects of expansion, contraction, and loss on substitution rates.

    Science.gov (United States)

    Zhu, Andan; Guo, Wenhu; Gupta, Sakshi; Fan, Weishu; Mower, Jeffrey P

    2016-03-01

    Rates of nucleotide substitution were previously shown to be several times slower in the plastid inverted repeat (IR) compared with single-copy (SC) regions, suggesting that the IR provides enhanced copy-correction activity. To examine the generality of this synonymous rate dependence on the IR, we compared plastomes from 69 pairs of closely related species representing 52 families of angiosperms, gymnosperms, and ferns. We explored the breadth of IR boundary shifts in land plants and demonstrate that synonymous substitution rates are, on average, 3.7 times slower in IR genes than in SC genes. In addition, genes moved from the SC into the IR exhibit lower synonymous rates consistent with other IR genes, while genes moved from the IR into the SC exhibit higher rates consistent with other SC genes. Surprisingly, however, several plastid genes from Pelargonium, Plantago, and Silene have highly accelerated synonymous rates despite their IR localization. Together, these results provide strong evidence that the duplicative nature of the IR reduces the substitution rate within this region. The anomalously fast-evolving genes in Pelargonium, Plantago, and Silene indicate localized hypermutation, potentially induced by a higher level of error-prone double-strand break repair in these regions, which generates substitutional rate variation. PMID:26574731

  19. Status and limiting factors of two rare plant species in dry montane communities of Hawai`i Volcanoes National Park.

    Science.gov (United States)

    Pratt, Linda W.; VanDeMark, Joshua R.; Euaparadorn, Melody

    2012-01-01

    Two rare plants native to montane dry forests and woodland communities of Hawai`i Volcanoes National Park (HAVO) were studied for more than two years to determine their stand structure, short-term mortality rates, patterns of reproductive phenology, success of fruit production, floral visitor composition, seed germination rates in the greenhouse, and survival of both natural and planted seedlings. Phyllostegia stachyoides, a shrubby Hawaiian mint (Lamiaceae) that is a species of concern, was studied within two small kīpuka at a natural population on the park’s Mauna Loa Strip, and three plantings at sites along the Mauna Loa Road were also monitored. Silene hawaiiensis, a threatened shrub species in the pink family (Caryophyllaceae), was monitored at two natural populations, one on Mauna Loa at the Three Trees Kīpuka and the second on Kīlauea Crater Rim south of Halema`uma`u. Silene hawaiiensis plantings were also made inside and outside ungulate exclosures at the park’s Kahuku Unit

  20. The genus Aphidura (Hemiptera, Aphididae in the collection of the Muséum national d’Histoire naturelle of Paris, with six new species

    Directory of Open Access Journals (Sweden)

    Juan M. Nieto Nafría

    2013-07-01

    Full Text Available Specimens were studied of 65 samples of the genus Aphidura (Aphididae, Aphidinae, Macrosiphini from the collection of the Muséum national d’Histoire naturelle (Paris. The possible synonymies of three pairs of species are discussed. New aphid host plant relationships are reported for A. bozhkoae, A. delmasi, A. ornata, A. pannonica and A. picta; this last species is recorded for first time from Afghanistan. The record of A. pujoli from Pakistan is refuted. The fundatrices, oviparous females and males of A. delmasi are described. Six new species are established: A. gallica sp. n. and A. amphorosiphon sp. n. from specimens caught on species of Silene (Caryophyllaceae from France and Iran, respectively, A. pakistanensis sp. n., A. graeca sp. n. and A. urmiensis sp. n. from specimens caught on species of Dianthus, Gypsophila and Spergula (Caryophyllaceae from Pakistan, Greece and Iran, respectively, and A. iranensis sp. n. from specimens caught on Prunus sp. from Iran. Modifications are made to the keys by Blackman and Eastop to aphids living on Dianthus, Gypsophyla, Silene, Spergula and Prinsepia and Prunus (Rosaceae. An identification key to apterous viviparous females of species of Aphidura is also provided.

  1. [Adhesion of Bacillus subtilis on the surface of pectin-calcium gel].

    Science.gov (United States)

    Gunter, E A; Melekhin, A K

    2015-01-01

    Pectin-calcium gels obtained based on pectins of callus cultures are able to adhere to the surface of cells of Gram-positive bacteria Bacillus subtilis to various degrees and this is thanks to the structural features of pectin. Rapid adhesion of the cells to gels obtained from the pectin of Tanacetum vulgare (TVC) callus cultures is associated with a high content of the linear region in the carbohydrate chain of pectin, a high molecular weight, and a low degree of methyl etherification of pectin. The number of adherent cells on the surface of gels obtained from pectins of Silene vulgaris callus cultures (SVC), TVC, and Lemna minor (LMC) after 8 h of incubation was close, whereas the number of cells was minimal on a gel produced using the pectin of Silene tatarica (STC) callus culture. This was due to the higher degree of methyl etherification of STC pectin (45%) compared to other pectins (4-12%). The adhesion rate constant (k) of B. subtilis for TCV gel during the first 120 min was the highest in comparison with other gels; the k value for SVC, STC and LMC gels was similar. The lowest level of k was characteristic for the gel from commercial apple pectin. The obtained data can beused for the production of gels with adhesive and antiadhesive properties. PMID:25842905

  2. Some Features of Raman Scattering by Molecules Adsorbed on Metal Crystal Faces and a Fine Light Structure

    CERN Document Server

    Polubotko, A M

    2013-01-01

    The paper analyzes some experiments on Raman scattering by molecules adsorbed on the face (111) of silver monocrystals performed by A. Campion et al. From the existence of the forbidden line of benzene, the conclusion about existence of the surface field, caused by atomic structure of the surface is made. The relatively large intensity of this line allows to make a conclusion about large influence of the electromagnetic field spatial inhomogeneity in crystals on their optical properties. The difference between this field and a regular plane wave, which usually describes propagation of electromagnetic field in solids is named as a fine light structure. The influence of this structure on optical properties of solids is pointed out.

  3. Angela Albanese, Metamorfosi del Cunto di Basile. Traduzioni, riscritture, adattamenti

    Directory of Open Access Journals (Sweden)

    Veronica Bonanni

    2013-05-01

    Full Text Available Angela Albanese ricostruisce in questo saggio la storia della ricezione del Cunto, prendendo in esame una campione di traduzioni interlinguistiche e intralinguistiche, riscritture, adattamenti per l’infanzia e trasposizioni intersemiotiche. Uno dei suoi meriti principali è sicuramente quello di esplicitare con chiarezza i criteri metodologici adottati, che conciliano in una prospettiva coerente i diversi apporti delle più moderne teorie sulla traduzione e la riscrittura, dalla critica «produttiva» di Antoine Berman ai paradigmi descrittivi e sistemici di Even-Zohar e Toury. Mettendo da parte un atteggiamento valutativo, che riduce la traduzione a copia necessariamente imperfetta dell’originale, Albanese considera il testo tradotto, appunto, come testo, dotato di una sua autonomia e coerenza, che va inserito nel contesto letterario e culturale in cui è prodotto. 

  4. Bestseller e generi letterari. Comparazioni testuali secondo i frame della solitudine e della coppia

    Directory of Open Access Journals (Sweden)

    Alessandro Ceteroni

    2015-07-01

    Full Text Available Che cosa significa studiare i bestseller? L’articolo si propone di rispondere alla domanda fornendo un modello teorico basato sulla comparazione testuale, sul riconoscimento di elementi costanti del discorso e sull’individuazione di uno stile cognitivo comune. Nell’articolo vengono condotte due analisi: la prima su un campione di otto romanzi di successo che raccontano vicende di solitudine; la seconda su quattro romanzi di successo che narrano storie di coppie esemplari. The paper proposes a theoretical model for bestseller research in the field of the textual analysis. It focuses on the comparision of semantic, syntactic and pragmatic constants, and the application of cognitive concepts. The paper examines two samples of novels telling about loneliness and couple stories respectively.

  5. Modelli di acquisto di e-book per le biblioteche dell'università italiana. Tre piattaforme a confronto (EBL, Ebrary, Ebsco

    Directory of Open Access Journals (Sweden)

    Giovanna Frigimelica

    2014-01-01

    Full Text Available Negli ultimi anni la diffusione degli e-book ha avuto una crescita esponenziale. L'accesso 24 ore su 24, la portabilità, gli strumenti avanzati offerti dalle piattaforme rendono questo prodotto particolarmente interessante in ambito accademico. Le biblioteche si trovano ad attraversare una fase di sperimentazione per quanto concerne la selezione e l'acquisizione degli e-book. L'articolo analizza tre piattaforme (EBL, Ebrary, EBSCO in particolare con l'ottica di stabilire se un'università italiana di medie dimensioni possa acquistare titoli stranieri in versione elettronica anziché cartacea, e a che condizioni. Vengono presentate informazioni sul catalogo offerto, i business model, le funzionalità disponibili. Il taglio è pratico/sperimentale, in quanto i paragoni tra le piattaforme si basano su un set di 150 monografie, pubblicate negli ultimi 5 anni da editori diversi e acquistate in cartaceo, utilizzato come campione rappresentativo.

  6. Air pollution abatement by road traffic and social acceptability. A case study; Misure anti-inquinamento da traffico urbano e partecipazione del cittadino: uno studio del caso

    Energy Technology Data Exchange (ETDEWEB)

    Giuntarelli, P.; Borrelli, G.; Brini, S. [ENEA, Centro Ricerche Casaccia, Rome (Italy). Dipt. Ambiente

    1999-07-01

    The paper describes the research, realised via a questionnaire on citizens representative of two roman districts, analyses the social acceptability degree of actions for reducing atmospheric pollution produced by automobile traffic in the city of Rome (Italy). [Italian] Il rapporto descrive una ricerca sul campo, svolta tramite questionario e su un campione di cittadini di due quartieri di Roma, che si propone di esaminare il grado di accettazione sociale di una serie di misure tendenti a ridurre l'inquinamento atmosferico causato dal traffico veicolare urbano, dal momento che trova unanime consenso riconoscere al traffico veicolare e piu' in generale al sistema di vita urbano le maggiori responsabilita' per quanto riguarda la produzione di sostanze inquinanti nell'aria.

  7. Investigating the Educational Use of Web2.0 Among Undergraduates in Nigerian Private Universities

    Directory of Open Access Journals (Sweden)

    Akorede Muftau Diyaolu

    2015-01-01

    Full Text Available Questo studio riporta i risultati di un sondaggio sull'uso di web2.0 tra gli studenti di due università private della Nigeria : la Crescent University di Abeokuta e la Caleb Unviersity di Lagos . La ricerca è finalizzata a trovare l' attuale consapevolezza degli studenti nell'uso del web2.0 , catturare il loro modello nell'utilizzo di web2.0 e determinare la sua rilevanza in ricerche accademiche . A tal fine, circa 161 studenti che rappresentano il 67,1 % dell'intero campione di studio hanno preso parte al sondaggio compilando un questionario. Il saggio fornisce un quadro dettagliato dei risultati con relative discussioni e raccomandazioni.

  8. A reflectivity profilometer for the optical characterisation of grade reflectivity mirrors in the 250 nm - 1100 nm spectral region

    Energy Technology Data Exchange (ETDEWEB)

    Colucci, Alessandro; Nichelatti, Enrico [ENEA, Centro Ricerche Casaccia, Rome (Italy). Dipt. Innovazione

    1998-04-01

    It`s developed the prototype of an instrument that can be used for the optical characterisation of graded reflectivity mirrors at any wavelength in the spectral region from 250 nm to 1100 nm. The instrument utilises a high-pressure Xe arc lamp as light source. Light is spectrally filtered by means of a grating monochromator. The sample is illuminated with an image of the monochromator exit slit. After reflection from the sample, this image is projected onto a 1024-elements charge-coupled device linear array driven by a digital frame board and interfaced with a personal computer. It`s tested the instrument accuracy by comparing measurement results with the corresponding ones obtained by means of a laser scanning technique. Measurement Rms repeatability has been estimated to be approximately of 0.8%. [Italiano] E` stato sviluppato il prototipo di uno strumento per la catatterizzazione ottica di specchi a riflettivita` variabile, operante a qualsiasi lunghezza d`onda nell`intervallo spettrale da 250 nm a 1100 nm. La sorgente dello strumento e` una lampada ad arco allo Xenon ad alta pressione. La luce e` filtrata spettralmente per mezzo di un monocromatore a reticolo. Il campione viene illuminato da un`immagine della fenditura d`uscita del monocromatore. Dopo essere stata riflessa dal campione, questa immagine viene proiettata su un array CCD lineare a 1024 elementi, connesso elettronicamente a una scheda digitale e interfacciato a un personal computer. L`accuratezza dello strumento e` stata verificata confrontando alcune misure con le corrispondenti misure ottenute mediante una tecnica a scansione laser. La ripetibilita` RMS delle misure e` stata stimata essere circa dello 0.8%.

  9. Variabilità morfologica ed ecologica in Neomys fodiens e Neomys anomalus nell'Appennino settentrionale

    Directory of Open Access Journals (Sweden)

    Dino Scaravelli

    2003-10-01

    Full Text Available I due Neomys italiani sono ancora da chiarire dal punto di vista della loro caratterizzazione morfologica e ecologica. Il lavoro prende in considerazione un campione di entrambe le specie proveniente da habitat forestali dell?Appennino settentrionale per i quali sono stati identificati i principali parametri ambientali. Vengono quindi descritte la variabilità dei tratti morfologici delle due specie in aree localizzate nel Parco Nazionale Foreste Casentinesi, Monte Falterona e Campigna nell?Appennino tosco-romagnolo. Risultano di sicuro effetto discriminatorio la maschera facciale, il rapporto piede posteriore/coda e i caratteri cranici. Sulla base dei criteri identificativi si sono realizzate rilevazioni di misure corporee per le due specie e una comparazione degli habitat utilizzati. N. fodiens appare unica specie nelle faggete-abetine e dominante nei castagneti, mentre nell?Ontaneta e nelle zone aperte e termofile si registra la sola presenza di N. anomalus. Mancano entrambi nei prati cespugliati, nella pecceta e nella cerreta. I gradienti presenti sono quindi illustrati. Non appare una differenza altitudinale nel campione esaminato, posto in stazioni tra i 400 e i 1300 m, ma per entrambe vi sono maggiori riscontri nella fascia tra 700 e 850 m. Nell?analisi multivariata rispetto alle altre specie e alle variabili ambientali si riscontra sempre una discreta correlazione con la presenza di acqua di una certa ampiezza, che comunque è significativa solo per N. fodiens, mentre risulta di interesse la positiva correlazione di N. anomalus con Apodemus sylvaticus.

  10. A whole plant approach to evaluate the water use of mediterranean maquis species in a coastal dune ecosystem

    Science.gov (United States)

    Mereu, S.; Salvatori, E.; Fusaro, L.; Gerosa, G.; Muys, B.; Manes, F.

    2009-02-01

    An integrated approach has been used to analyse the water relations of three Mediterranean species, A. unedo L., Q. ilex L. and P. latifolia L. co-occurring in a coastal dune ecosystem. The approach considered leaf level gas exchange, sap flow measurements and structural adaptations between 15 May and 31 July 2007, and was necessary to capture the different response of the three species to the same environment. The complexity of the response was proportional to the complexity of the system, characterized by a sandy soil with a low water retention capacity and the presence of a water table. The latter did not completely prevent the development of a drought response, and species differences in this responses have been partially attributed to a different root distribution. Sap flow of A. unedo decreased rapidly in response to the decline of Soil Water Content, while that of Q. ilex decreased only moderately. Midday leaf water potential of P. latifolia and A. unedo was between 2.2 and 2.7 MPa through the measuring period, while in Q. ilex it reached a value of 3.4 MPa at the end of the season. A. unedo was the only species to decrease the leaf area to sapwood area ratio from 23.9±1.2 (May) to 15.2±1.5 (July), as a response to drought. A. unedo also underwent an almost stepwise loss on hydraulic conductivity, such a loss didn't occur for Q. ilex, while P. latifolia was able to slightly increase hydraulic conductivity, showing how different plant compartments coordinate differently between species as a response to drought. Such different coordination affects the gas exchange between vegetation and the atmosphere, and has implications for the response of the Mediterranean coastal dune ecosystems to climate change.

  11. An integrated approach shows different use of water resources from Mediterranean maquis species in a coastal dune ecosystem

    Science.gov (United States)

    Mereu, S.; Salvatori, E.; Fusaro, L.; Gerosa, G.; Muys, B.; Manes, F.

    2009-11-01

    An integrated approach has been used to analyse the dependence of three Mediterranean species, A. unedo L., Q. ilex L., and P. latifolia L. co-occurring in a coastal dune ecosystem on two different water resources: groundwater and rainfed upper soil layers. The approach included leaf level gas exchanges, sap flow measurements and structural adaptations between 15 May and 31 July 2007. During this period it was possible to capture different species-specific response patterns to an environment characterized by a sandy soil, with a low water retention capacity, and the presence of a water table. The latter did not completely prevent the development of a drought response and, combined with previous studies in the same area, response differences between species have been partially attributed to different root distributions. Sap flow of A. unedo decreased rapidly with the decline of soil water content, while that of Q. ilex decreased only moderately. Midday leaf water potential of P. latifolia and A. unedo ranged between -2.2 and -2.7 MPa throughout the measuring period, while in Q. ilex it decreased down to -3.4 MPa at the end of the season. A. unedo was the only species that responded to drought with a decrease of its leaf area to sapwood area ratio from 23.9±1.2 (May) to 15.2±1.5 (July). While A. unedo also underwent an almost stepwise loss on hydraulic conductivity, such a loss did not occur for Q. ilex, whereas P. latifolia was able to slightly increase its hydraulic conducitivity. These differences show how different plant compartments coordinate differently between species in their responses to drought. The different responses appear to be mediated by different root distributions of the species and their relative resistances to drought are likely to depend on the duration of the periods in which water remains extractable in the upper soil layers.

  12. Contrasting impacts of continuous moderate drought and episodic severe droughts on the aboveground-biomass increment and litterfall of three coexisting Mediterranean woody species.

    Science.gov (United States)

    Liu, Daijun; Ogaya, Romà; Barbeta, Adrià; Yang, Xiaohong; Peñuelas, Josep

    2015-11-01

    Climate change is predicted to increase the aridity in the Mediterranean Basin and severely affect forest productivity and composition. The responses of forests to different timescales of drought, however, are still poorly understood because extreme and persistent moderate droughts can produce nonlinear responses in plants. We conducted a rainfall-manipulation experiment in a Mediterranean forest dominated by Quercus ilex, Phillyrea latifolia, and Arbutus unedo in the Prades Mountains in southern Catalonia from 1999 to 2014. The experimental drought significantly decreased forest aboveground-biomass increment (ABI), tended to increase the litterfall, and decreased aboveground net primary production throughout the 15 years of the study. The responses to the experimental drought were highly species-specific. A. unedo suffered a significant reduction in ABI, Q. ilex experienced a decrease during the early experiment (1999-2003) and in the extreme droughts of 2005-2006 and 2011-2012, and P. latifolia was unaffected by the treatment. The drought treatment significantly increased branch litterfall, especially in the extremely dry year of 2011, and also increased overall leaf litterfall. The drought treatment reduced the fruit production of Q. ilex, which affected seedling recruitment. The ABIs of all species were highly correlated with SPEI in early spring, whereas the branch litterfalls were better correlated with summer SPEIs and the leaf and fruit litterfalls were better correlated with autumn SPEIs. These species-specific responses indicated that the dominant species (Q. ilex) could be partially replaced by the drought-resistant species (P. latifolia). However, the results of this long-term study also suggest that the effect of drought treatment has been dampened over time, probably due to a combination of demographic compensation, morphological and physiological acclimation, and epigenetic changes. However, the structure of community (e.g., species composition

  13. Callus formation impedes adventitious rhizogenesis in air layers of broadleaved tree species

    Directory of Open Access Journals (Sweden)

    Sanjay Singh

    2014-07-01

    Full Text Available Callusing and root induction in air layering was evaluated aiming at evolution of procedure for mass clonal propagation of mature ortets of five tropical broadleaf species differing in their potential for adventitious root formation in shoot cuttings as: Anogiessus latifolia < Boswellia serrata < Dalbergia latifolia < Gmelina arborea < Dalbergia sissoo. Two experiments were conducted in rainy season during consecutive years; without application of growth regulators in the first year and with growth regulators (T1 - water, T2- 100 ppm indole-3-acetic acid, T3-100 ppm thiamine-HCl and T4 -combination of T2 + T3 in the next year. Air layered branches were detached from the trees to record percentage of alive airlayers, callusing and rooting (% as well as root number and root length. Response to air layering was found to be highly variable in five tree species but appeared to be feasible procedure for clonal propagation of mature ortets of B. serrata and D. sissoo with 100% (in auxin + thiamine treatment and 83.3% (in auxin treatment success, respectively. Maximum callusing (% was found in D. latifolia while no callusing was observed in D. sissoo, which is most easy-to-root among all five species. Callus formation impedes adventitious rhizogenesis in air layers as significant negative correlation of callusing (% and adventitious root formation was recorded in air layers of five tropical broadleaved tree species. Application of exogenous auxin alone or in combination with thiamine circumvents callusing to ensure direct development of roots for successful air layering.

  14. Chemical constituents of selected Sudanese medicinal and aromatic plants

    International Nuclear Information System (INIS)

    Sudanese medicinal and aromatic plants (Alternanthra repens, Ambrosia maritima, Citrus paradisi, Croton zambesicus, Lepidium sativum, Morettia phillaena, Nauclea latifolia, Plectranthus barbatus, Pluchea dioscorides, and Sphaeranthus suaveolens) were analyzed for their chemical composition, mineral contents and secondary constituents. The concentration of manganese, copper, iron, nickel, lead, zinc and potassium in plant samples was performed using x-ray fluorescence spectrometry. The trace elements found in the smallest amount of the investigated plant species are lead, nickel and copper, while high concentration was detected for potassium, iron and manganese. Mn was accumulated with high level in Alternanthra repens species. Potassium was abundant in S. suaveolens and Ambrosia maritima. The values of concentration obtained for all studied elements were compared with published values of reference material, trace elements in Hay (powder) by International Atomic Energy Agency. Phyto chemical analysis of investigated plants was performed for constituents: Flavonoids, saponins, tannins, alkaloids, amino acids and sugars. The methanolic extracts of P.barbatus, C.paradisi, A.repens, N.latifolia, L. sativum and C. zambesicus are found to contain alkaloids. Results of TLC analysis were shown as Rf values for saponins, bitter principles, essential oils, flavonoids and alkaloids. Quantification of flavonoids and tannins showed that flavonoid content was highest in case of Alternanthera repens and Sphaeranthus suavertens, whereas the highest tannin content was in case of Nauclea latifolia and Sphaearanthus suavertens. The results suggest that the user of traditional Sudanese crude drugs should be warned of potential danger of heavy metal poisoning because their concentrations seem to be higher than maximum values allowed by health agencies in several countries. This study has provided some biochemical basis for the ethno medical use of extracts from different candidate plant

  15. THE FLORA OF HEKIMDAG (BOZDAG, ESKISEHIR

    Directory of Open Access Journals (Sweden)

    Murat ARDIÇ

    2011-02-01

    Full Text Available In this study the flora of Eskişehir Hekimdağ (Bozdağ were investigated. According to Davis's grid system, the research area lies in B3 square. The plant samples collected in this region and determined that there are 59 families, as well as 467 species and subspecies taxa belonging to 219 genera. The phytogeographical distribution is as follows: 49 Mediterranean, 31 Euro-Siberian, 19 Irano-Turanian, 6 Euxin and 362 unknown or other-regional elements. The largest families are Asteraceae (50, Fabaceae (38, Lamiaceae (24, Poaceae (21 and Liliaceae (18 in terms of the number of species and subspecies included. Genera represented by the highest number of species are Centaurea (7, Alyssum (6, Silene, Astragalus, Trifolium, Lathyrus ve Campanula (5 . The number of endemic taxa within the study area is 41 (10.86%. 13 taxa are new records for the B3 square.

  16. Improved phytoaccumulation of cadmium by genetically modified tobacco plants (Nicotiana tabacum L.). Physiological and biochemical response of the transformants to cadmium toxicity

    International Nuclear Information System (INIS)

    The response of tobacco plants (Nicotiana tabacum L.)-non-transformed and transformed with a metallothionein gene MThis from Silene vulgaris L. - to increase cadmium supply in the nutrient solution was compared. The transgenic plants accumulated significantly more Cd both in the roots and the leaves. Visual toxicity symptoms and disturbance in water balance were correlated with Cd tissue content. Treatment with 300 μM CdCl2 resulted in inhibition of photosynthesis and mobilization of the ascorbate-glutathione cycle. Treatment with 500 μM CdCl2 led to irreversible damage of photosynthesis and oxidative stress. An appearance of a new peroxidase isoform and changes in the leaf polypeptide pattern were observed at the highest Cd concentration. The level of non-protein thiols gradually increased following the Cd treatment both in transgenic and non-transformed plants. - Genetic transformation of Nicotiana tabacum L. by metallothionein gene improved phytoaccumulation of cadmium

  17. Conceptual design and sampling procedures of the biological programme of NuukBasic

    DEFF Research Database (Denmark)

    Aastrup, Peter; Nymand, Josephine; Raundrup, Katrine;

    Vegetation Index (NDVI). The fl ux of CO2 is measured in natural conditions as well as in manipulations simulating increased temperature, increased cloud cover, shorter growing season, and longer growing season. The effect of increased UV-B radiation on plant stress is studied by measuring chlorophyll fl...... vegetation transect, and monitoring reproductive phenology of Salix glauca, Loiseleuria procumbens, Eriophorum angustifolium, and Silene acaulis. The progression in vegetation greenness is followed along the vegetation transect and in the plant phenology plots by measurement of Normalized Diff erence...... terrestrial mammals occur in the study area. All observations of mammals are recorded ad-hoc. Monitoring in lakes include ice cover, water chemistry, physical conditions, species composition of plankton, vegetation, bottom organisms and fi sh. Physical-chemical parameters, phytoplankton and zooplankton...

  18. Targetingβ-secretase with RNAi in neural stem cells for Alzheimer’s disease therapy

    Institute of Scientific and Technical Information of China (English)

    Zhonghua Liu; Shengliang Li; Zibin Liang; Yan Zhao; Yulin Zhang; Yaqi Yang; Minjuan Wang; Feng Li

    2013-01-01

    There are several major pathological changes in Alzheimer’s disease, including apoptosis of cho-linergic neurons, overactivity or overexpression ofβ-site amyloid precursor protein cleaving enzyme 1 (BACE1) and inflammation. In this study, we synthesized a 19-nt oligonucleotide targeting BACE1, the key enzyme in amyloid beta protein (Aβ) production, and introduced it into the pSilenCircle vector to construct a short hairpin (shRNA) expression plasmid against the BACE1 gene. We transfected this vector into C17.2 neural stem cells and primary neural stem cells, resulting in downregulation of the BACE1 gene, which in turn induced a considerable reduction in reducing Aβprotein production. We anticipate that this technique combining celltransplantation and gene ther-apy wil open up novel therapeutic avenues for Alzheimer’s disease, particularly because it can be used to simultaneously target several pathogenetic changes in the disease.

  19. Irradiated fuel behavior under accident heating conditions and correlation with fission gas release and swelling model (Chicago)

    International Nuclear Information System (INIS)

    We analyse the mixed oxide fast fuel response to off normal conditions obtained by means of an out-of-pile transient simulation apparatus designed to provide direct observations (temperature, pressure, fuel motion) of fuel fission gas phenomena that might occur during the transients. The results are concerning fast transient tests (0,1 to 1 second) obtained with high gas concentration irradiated fuel (4 to 7 at % burn up, 0,4 cm3Xe + Kr /g.UPuO2). The kinetics of fission gas release during the transients have been directly measured and then compared with the calculated results issued of the Chicago model. This model agrees, quite well, with other experiments done in the silene prompt reactor. Other gases than xenon and krypton (such as hydrogen and carbon monoxide) do not play any role in fuel behavior, since they have been completely ruled out

  20. Arbuscular mycorrhiza and plant succesion on zinc smelter spoil heap in Katowice-Wełnowiec

    Directory of Open Access Journals (Sweden)

    Ewa Gucwa-Przepióra

    2014-02-01

    Full Text Available Mycorrhizal status of plants colonising the zinc wastes in Katowice was surveyed. In total 69 vascular plant species (25 families appearing on the investigated area have been noted. More than 60% of them were mycorrhizal. Non-mycorrhizal species, such as Cardaminopsis arenosa and Silene vulgaris dominated the early successional part of the zinc heap. Tussilago farfara was the only AM plant there, however, no arbuscules were developed at this stage. The number of AM species was increased on the 20 years old part of the zinc wastes and on the older 30-50 years old areas. AM plants constituted about 60% of the total number of species there. The frequency of particular AM species was the highest on the oldest part of the investigated area. The usefulness of the results for restoration practices was discussed.

  1. Sementes nocivas que ocorreram em amostras de sementes de azevém (Lolium multiflorum, analisadas no Rio Grande do Sul nos anos de 1978 e 1979 Weed seeds in ryegrass seeds, analysed in Rio Grande do Sul, during 1978 and 1979

    Directory of Open Access Journals (Sweden)

    H. Giaretta

    1984-06-01

    Full Text Available Foram levantadas informações sobre a qualidade da semente de azevém utilizadas no Rio Grande do Sul e produzida no próprio Estado ou em outras uni dades da Federação e mesmo em outros Países, nos anos de 1978 e 1979. Estas informações foram obtidas através de fichas e Boletins de Análise de Sementes dos Laboratórios de Análise de Sementes (LAS do Rio Grande do Sul. Em 1978 analisou-se 2.319 t de azevém sendo 74% da semente oriunda do Rio Grande do Sul e 26% introduzida, enquanto que das 4.772 t analisadas em 1979 99,6% são do Rio Grande do Sul e 0,4% são introduzidas. O percentual de sementes de azevém, produzidas no Estado, contaminadas com sementes nocivas foi de 61,5% em 1978 e de 60,0% em 1979; e o de sementes int roduzidas no Estado foi de 45,6% em 1978 e de 29,4%em 1979. Foi observado que ent re as sementes originárias do RS destacaram -se com maior ocorrênci a em 1978 as espécies nocivas de Silene gallica, Setaria geniculata, Anthemis cotula, Digitaria adscendens e Echinochloa spp, enquanto que nas sementes int roduzidas desta caram-se Sida spp e Rumex spp; em 1979, na semente oriunda do Estado desta caram-se Amaranthus spp, Silene gallica e Setaria geniculata, enquanto que nas sementes introduzidas a maior ocorrência foi de Setaria geniculata, Echinochloa spp e Solanum spp.This paper presents a quality record of rye-grass seeds produced in Rio Grande do Sul or imported from other countries. It refers to the presence of weeds in ryegrass during 1978 and 1979. These record were obtained in the Analysis Bulletins at the Seed Analysis Laboratory of the Instituto de Pesquisas Agronômicas (IPAGRO Rio Grande do Sul°Brasil and in those from other institutions. This State produced 74% of 2.319 t analysed seeds and 26% come from other places in 1978. In 1979 4.772 t were registred, 99,6% from here and 0,4% from outside. The percentage of ryegrass seeds contaminated with weed seeds produced in the State, in 1978, were 61

  2. Cytotoxic effects of selective species of Caryophyllaceae in Iran

    Directory of Open Access Journals (Sweden)

    F. Naghibi

    2014-04-01

    Full Text Available Cancer is a major cause of death worldwide and causes serious problems in human life. It is developed by uncontrolled growth of a cell or a group of cells. There are many difficulties in treatment of cancer and many researchers are involved in investigating for effective drugs to treat the disease. Caryophyllaceae is a large family of about 86 genera and 2200 herbaceous or subshrub species. The family is known for its ornamental plants and saponin compounds. In the present study, the potential cytotoxic activity of 17 selected species from Caryophyllaceae has been investigated against MCF-7, HepG-2, A-549, HT-29 and MDBK cells using MTT assay. Five species exhibited cytotoxic effects with IC50 values < 100 μg/mL. Silene ampullata and Acanthophyllum bracteatum extracts were toxic only against MCF-7 cell line suggesting them as suitable candidates for more investigations of breast cancer studies.

  3. Biosynthesis of Cd-bound phytochelatins by Phaeodactylum tricornutum and their speciation by size-exclusion chromatography and ion-pair chromatography coupled to ICP-MS.

    Science.gov (United States)

    Loreti, Valeria; Toncelli, Daniel; Morelli, Elisabetta; Scarano, Gioacchino; Bettmer, Jörg

    2005-10-01

    Cd-bound phytochelatins (Cd-PCs) have been synthesised by incubation of Phaeodactylum tricornutum cell cultures with Cd and purified by size-exclusion chromatography-UV-Vis. These complexes, which were identified in previous work, have now been used as model substances to develop and optimise ion-pair chromatography (IPC) coupled to inductively coupled plasma-mass spectrometry (ICP-MS) for analysis of Cd-PCs. Subsequent analysis of samples taken from Silene vulgaris plants cultivated under heavy metal stress conditions revealed Cd signals but no Cd-PC signals. By use of isotopically enriched (116)Cd-PCs the sample preparation steps were verified to determine the stability of the analytes. We observed species transformation between Cd-PCs and other unidentified Cd complexes. Consequently, the kinetic and thermodynamic lability of Cd-PCs are decisive factors in their detection.

  4. Induction of flowering by 5-azacytidine in some plant species: relationship between the stability of photoperiodically induced flowering and flower-inducing effect of DNA demethylation.

    Science.gov (United States)

    Kondo, Hiroshi; Miura, Takashi; Wada, Kaede C; Takeno, Kiyotoshi

    2007-11-01

    The flower-inducing effect of 5-azacytidine, a DNA demethylating reagent, was examined in several plant species with a stable or unstable photoperiodically induced flowering state under non-inductive photoperiodic conditions. The long day plant Silene armeria, whose flowering state is stable and the short day plant Pharbitis nil, whose flowering state is unstable were induced to flower by 5-azacytidine under a non-inductive condition. Thus, the replacement of photoinduction by 5-azacytidine treatment is not specific to Perilla frutescens. On the other hand, 5-azacytidine did not induce flowering in Xanthium strumarium whose flowering state is stable and Lemna paucicostata whose flowering state is unstable. Thus, epigenetics caused by DNA demethylation may be involved in the regulation of photoperiodic flowering irrespective of the stability of the photoperiodically induced flowering state. PMID:18251884

  5. Fissile solution dynamics: Student research

    Energy Technology Data Exchange (ETDEWEB)

    Hetrick, D.L.

    1994-09-01

    There are two research projects in criticality safety at the University of Arizona: one in dynamic simulation of hypothetical criticality accidents in fissile solutions, and one in criticality benchmarks using transport theory. We have used the data from nuclear excursions in KEWB, CRAC, and SILENE to help in building models for solution excursions. An equation of state for liquids containing gas bubbles has been developed and coupled to point-reactor dynamics in an attempt to predict fission rate, yield, pressure, and kinetic energy. It appears that radiolytic gas is unimportant until after the first peak, but that it does strongly affect the shape of the subsequent power decrease and also the dynamic pressure.

  6. Laser induced spectroscopy breakdown (LIBS) application to heavy metal detection in soils; Applicazioni della tecnica Laser induced breakdown spectroscopy (LIBS) alla determinazione dei metalli pesanti nei suoli

    Energy Technology Data Exchange (ETDEWEB)

    Barbini, R.; Fantoni, R.; Palucci, A.; Ribezzo, S.; Colao, F. [ENEA, Centro Ricerche Frascati, Frascati, RM (Italy). Div. Fisica Applicata; Capitelli, F. [Bari, Univ., Bari (Italy). Dipt. di Biologia e Chimica Agroforestale ed Ambientale

    2000-07-01

    and the sample upon which the analytic use of LIBS technique relays. RSD % (Relative Standard Deviation) for LIBS and ICP data have been compared. LIBS values appeared systematically higher than the ICP ones. Also the correlation coefficients, calculated as the ratio between certified value and experimental one, both for LIBS and ICP, were considerably less close to one for LIBS than for ICP. In order to minimize the matrix effect, a model for correcting LIBS data by taking into account the plasma temperature experimentally determined both in the unknown and in the standard sample was introduced. Preliminary results showed a considerable improvement in the correlation coefficients for corrected LIBS data. Once the model validity is proven for different elements used in the plasma temperature determination, which is possible once known all the parameters of Boltzmann equation for experimental intensity spectral line, it is quite general and can be applied on all the samples and for all the elements. [Italian] Il LIBS (Laser induced breakdown spectroscopy) e' una tecnica spettroscopica sperimentale che trova applicazione nell'analisi di campioni di interesse ambientale, quali suoli e rocce, ed industriali, come leghe metalliche. In questo rapporto sono presentati i risultati dell'applicazione della tecnica a campioni eterogenei di suoli certificati dal Joint Research Centre di Ispra (Varese) per la valutazione delle concentrazioni di metalli pesanti di interesse agronomico (Cd, Cr, Cu, Fe, Mn, Ni, Pb, Zn). Nella tecnica LIBS, un impulso laser d'alta potenza e' focalizzato sul campione in modo da provocare sulla superficie dello stesso un piccolo volume di plasma. Le emissioni delle singole specie atomiche sono raccolte da fibra ottica e analizzate da un monocromatore e da un analizzatore ottico (iCCD). Il riconoscimento e l'assegnazione delle emissioni spettrali corrispondenti ai singoli elementi atomici permette di riconoscere la

  7. Evaluación de extractos y fracciones de plantas colombianas en modelos de inflamación aguda, subcrónica y crónica

    OpenAIRE

    Gonzalez, María Cristina; Ospina, Luis Fernando; Calle, Jairo; Rincon, Javier

    2008-01-01

    Se evaluó la actividad antiinflamatoria de extractos y fracciones de Acnistus arborescens, Baccharis latifolia, Myrcianthes leucoxila, Physalis peruviana y Salvia rubescens en los modelos in vivo de inflamación edema tópico en oreja de ratón, edema plantar por carragenina en rata y bolsa de aire en ratón, con profundización en modelo de artritis por adyuvante en rata. Inicialmente se realizó el screening de un total de 34 fracciones en el modelo de edema auricular en ratón, de los cuales se s...

  8. Dinâmica populacional de plantas daninhas sob diferentes sistemas de manejo nas culturas de milho e feijão Weed population dynamics under different corn and bean production systems

    OpenAIRE

    A. Jakelaitis; L.R. Ferreira; A.A. Silva; E.L. Agnes; G.V. Miranda; A.F.L. Machado

    2003-01-01

    Com o objetivo de avaliar a dinâmica populacional de plantas daninhas por meio de parâmetros fitossociológicos, realizou-se este trabalho com as culturas de milho e feijão em cultivos sucessivos, no período de novembro de 1998 a maio de 2001, em um Argissolo Vermelho-Amarelo Câmbico, fase terraço, em Viçosa-MG. A comunidade de plantas daninhas era composta por Amaranthus deflexus, Brachiaria plantaginea, Cyperus rotundus, Galinsoga parviflora, Mucuna aterrima e Oxalis latifolia. Os tratamento...

  9. Typifications and synonymy in Polystichum (Dryopteridaceae) from Chile and Argentina

    Science.gov (United States)

    Morero, Rita E.; Barrington, David S.; McHenry, Monique A.; Condack, João P. S.; Barboza, Gloria E.

    2016-01-01

    Abstract Polystichum Roth is one of the largest and most taxonomically challenging fern genera. South American species have a rich and complex nomenclatural history; many of the early names are inadequately typified. Based on extensive examination of original type material, we designate eleven lectotypes (including Aspidium mohrioides, Aspidium montevidense f. imbricata, Aspidium montevidense f. squamulosa, Aspidium plicatum, Aspidium pycnolepis, Dicksonia andina, Polystichum elegans, Polystichum mohrioides f. latifolia, Polystichum multifidum var. autranii, Polystichum platyphyllum var. kurtziana, and Polypodium polystichoides), and one neotype (Polystichum brongniartianum) for Polystichum taxa. Furthermore, three new synonyms are proposed. PMID:27489490

  10. Potencial del banco de semillas en la regeneración de la vegetación del humedal jaboque, bogotá, colombia

    OpenAIRE

    MONTENEGRO-S., ALBA L.; ÁVILA PARRA, YUDY A.; MENDIVELSO-CH., HOOZ A.; Vargas, Orlando

    2013-01-01

    Se estudió el banco de semillas germinable (BS) en seis parches de vegetación en lazona conservada del humedal Jaboque: 1) Rumex conglomeratus - Polygonum sp. 2)Juncus effusus - Polygonum sp. 3) Typha latifolia 4) Juncus effusus 5) Pennisetumclandestinum - Polygonum sp. 6) Pennisetum clandestinum – Holcus lanatus. En cadaparche se evaluó la composición, la densidad de las especies presentes en el BS) el tipode BS que poseen algunas de las especies nativas del humedal) la similitud entre lacom...

  11. Eficiência do triclopyr no controle de plantas daninhas em gramado (Paspalum notatum) Triclopyr efficiency in controlling weeds in the lawn (Paspalum notatum)

    OpenAIRE

    F.C.L. Freitas; L. R. Ferreira; Silva, A. A.; J.G. Barbosa; G.V. Miranda; A.F.L. Machado

    2003-01-01

    As plantas daninhas interferem nos gramados, prejudicando a sua formação, qualidade e estética. O carrapicho-beiço-de-boi (Desmodium incanum) e a zórnia (Zornia latifolia), quando não controlados, podem atingir níveis de infestação que chegam a dizimar o gramado. Dentre as diversas técnicas de manejo de plantas daninhas em gramados, destaca-se o emprego de herbicidas; todavia, as informações sobre a utilização desses produtos em gramados são escassas. O objetivo deste trabalho foi avaliar a e...

  12. Diversidad de la fauna de artropodos terrestres en el humedal jaboque, bogotá-colombia

    OpenAIRE

    SÁNCHEZ-N., DAVID; AMAT-GARCÍA, GERMÁN D.

    2012-01-01

    En el Humedal Jaboque, ubicado dentro del perímetro urbano de la ciudad de Bogotá, se estudió la composición taxonómica y trófica, la riqueza y la diversidad de la fauna de artrópodos terrestres asociada a cinco comunidades de vegetación cuyas especies dominantes eran: Juncus effusus, Juncus effusus y Polygonum punctatum; Schoenoplectus californicus, Typha latifolia y Bidens laevis. Se colectaron 8944 individuos que representan una biomasa seca de 9757.8 mg., correspondientes a 212 morfoespec...

  13. Caracterización de la artropofauna terrestre del humedal jaboque (bogotá, colombia)

    OpenAIRE

    Amat García Germán; Sánchez Núñez David Alejandro

    2012-01-01

    En el humedal Jaboque, se estudió la diversidad y la estructura trófica de la artropofauna asociada a las comunidades de vegetación Juncus effusus, Juncus effusus-Polygonum punctatum, Schoenoplectus californicus y Typha latifolia. La composición y riqueza asociada a la comunidad Bidens laevis y la composición de los insectos antófilos relacionados a algunas plantas. Se identificaron en total 212 morfoespecies, distribuidas en 48 familias y siete órdenes. A lo largo del estudio se colectaron 8...

  14. A karyotypic and anatomical study of an unidentified liliaceous plant

    Directory of Open Access Journals (Sweden)

    J. J. Spies

    1983-12-01

    Full Text Available An unidentified sterile liliaceous plant and three possible relatives were studied karyotypically and anatomically. All these plants have a somatic chromosome number of 2n = 14. The possibility of the sterile plant having arisen as a result of a mutation appears unlikely, when compared with the possibility of hybrid origin. Chromosome morphology rules out  Bulbine latifolia (L.f. R. & S. and Aloe arborescens Mill, as possible parents. The sterile plant and Aloe marlothii Berger have similar karyotypes and. therefore. A. marlothii may be one of the parents. A close relationship between the sterile plant and the genus Aloe is further confirmed by their similar epidermal structure.

  15. An integrated approach shows different use of water resources from Mediterranean maquis species in a coastal dune ecosystem

    OpenAIRE

    Mereu, S; Salvatori, E.; L. Fusaro; G. Gerosa; Muys, B.; Manes, F

    2009-01-01

    An integrated approach has been used to analyse the dependence of three Mediterranean species, A. unedo L., Q. ilex L., and P. latifolia L. co-occurring in a coastal dune ecosystem on two different water resources: groundwater and rainfed upper soil layers. The approach included leaf level gas exchanges, sap flow measurements and structural adaptations between 15 May and 31 July 2007. During this period it was possible to capture differ...

  16. Germinación en el laboratorio de varias especies arbustivas mediterráneas : efecto de la temperatura

    OpenAIRE

    Salvador, Raimon; Lloret Maya, Francisco

    1995-01-01

    Después de la aplicación de los tratamientos de temperatura (control, 75°C 1 hora y 120°C 5 minutos), la germinación en el laboratorio de las semillas de las especies estudiadas ha sido diferente en cada una de ellas. Los tratamientos de temperatura han producido una estimulación significativa de la germinación sólo en Phyllirea latifolia, un arbusto rebrotador, y en Cistus salviifolius, una especie tipicamente germinadora y más raramente rebrotadora. En esta última especie, sin embargo, el t...

  17. ECOLOGICAL STATUS, DIVERSITY RESOURCES AND DISTRIBUTION OF THE LITTLE KNOWN GENUS TAINIA BLUME (ORCHIDACEAE IN ASSAM OF NORTH EAST INDIA

    Directory of Open Access Journals (Sweden)

    Khyanjeet Gogoi¹, Raju Das² and Rajendra Yonzone³

    2012-06-01

    Full Text Available Among the Orchid flora of Assam, four species of terrestrial Orchid Tainia recorded viz., T. angustifolia, T. latifolia, T. minor and T. wrayana in an intensive field survey during 1996-2010. The present paper deals Tainia species diversity and distribution in Assam of North East India. This attempt is the first step to correct taxonomic identification to workout currently accepted botanical names with present ecological status, date of collection, habitat, altitudinal ranges, phenology and local and general distribution of Tainia species in the regions.

  18. A multi-source portable light emitting diode spectrofluorometer.

    Science.gov (United States)

    Obeidat, Safwan; Bai, Baolong; Rayson, Gary D; Anderson, Dean M; Puscheck, Adam D; Landau, Serge Y; Glasser, Tzach

    2008-03-01

    A portable luminescence spectrofluorometer weighing only 1.5 kg that uses multiple light emitting diodes (LEDs) as excitation sources was developed and evaluated. Excitation using a sequence of seven individual broad-band LED emission sources enabled the generation of excitation-emission spectra using a light weight (pistache or Mastic) and Philyria latifolia, and the herbaceous species Medicago sativa (alfalfa), Trifolium spp. (clover), and a feed concentrate. Application of multi-way principal component analysis (MPCA) to the resulting three-dimensional data sets enabled discernment among these various diet constituents. PMID:18339242

  19. Assessing intraspecific variation in effective dispersal along an altitudinal gradient: a test in two Mediterranean high-mountain plants.

    Directory of Open Access Journals (Sweden)

    Carlos Lara-Romero

    Full Text Available BACKGROUND: Plant recruitment depends among other factors on environmental conditions and their variation at different spatial scales. Characterizing dispersal in contrasting environments may thus be necessary to understand natural intraspecific variation in the processes underlying recruitment. Silene ciliata and Armeria caespitosa are two representative species of cryophilic pastures above the tree line in Mediterranean high mountains. No explicit estimations of dispersal kernels have been made so far for these or other high-mountain plants. Such data could help to predict their dispersal and recruitment patterns in a context of changing environments under ongoing global warming. METHODS: We used an inverse modelling approach to analyse effective seed dispersal patterns in five populations of both Silene ciliata and Armeria caespitosa along an altitudinal gradient in Sierra de Guadarrama (Madrid, Spain. We considered four commonly employed two-dimensional seedling dispersal kernels exponential-power, 2Dt, WALD and log-normal. KEY RESULTS: No single kernel function provided the best fit across all populations, although estimated mean dispersal distances were short (<1 m in all cases. S. ciliata did not exhibit significant among-population variation in mean dispersal distance, whereas significant differences in mean dispersal distance were found in A. caespitosa. Both S. ciliata and A. caespitosa exhibited among-population variation in the fecundity parameter and lacked significant variation in kernel shape. CONCLUSIONS: This study illustrates the complexity of intraspecific variation in the processes underlying recruitment, showing that effective dispersal kernels can remain relatively invariant across populations within particular species, even if there are strong variations in demographic structure and/or physical environment among populations, while the invariant dispersal assumption may not hold for other species in the same environment

  20. Nectar sugar composition of European Caryophylloideae (Caryophyllaceae) in relation to flower length, pollination biology and phylogeny.

    Science.gov (United States)

    Witt, T; Jürgens, A; Gottsberger, G

    2013-10-01

    Floral nectar composition has been explained as an adaptation to factors that are either directly or indirectly related to pollinator attraction. However, it is often unclear whether the sugar composition is a direct adaptation to pollinator preferences. Firstly, the lower osmolality of sucrose solutions means that they evaporate more rapidly than hexose solutions, which might be one reason why sucrose-rich nectar is typically found in flowers with long tubes (adapted to long-tongued pollinators), where it is better protected from evaporation than in open or short-tubed flowers. Secondly, it can be assumed that temperature-dependent evaporation is generally lower during the night than during the day so that selection pressure to secrete nectar with high osmolality (i.e. hexose-rich solutions) is relaxed for night-active flowers pollinated at night. Thirdly, the breeding system may affect selection pressure on nectar traits; that is, for pollinator-independent, self-pollinated plants, a lower selective pressure on nectar traits can be assumed, leading to a higher variability of nectar sugar composition independent of pollinator preferences, nectar accessibility and nectar protection. To analyse the relations between flower tube length, day vs. night pollination and self-pollination, the nectar sugar composition was investigated in 78 European Caryophylloideae (Caryophyllaceae) with different pollination modes (diurnal, nocturnal, self-pollination) using high-performance liquid chromatography (HPLC). All Caryophylleae species (Dianthus and relatives) were found to have nectar with more than 50% sucrose, whereas the sugar composition of Sileneae species (Silene and relatives) ranged from 0% to 98.2%. In the genus Silene, a clear dichotomous distribution of sucrose- and hexose-dominant nectars is evident. We found a positive correlation between the flower tube length and sucrose content in Caryophylloideae, particularly in day-flowering species, using both conventional

  1. Valutazione dell'affidabilità degli operatori per l'identificazione microscopica di peli di mammiferi

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    Paolo Ciucci

    2003-10-01

    Full Text Available La quantificazione dei resti indigesti contenuti negli escrementi è tra le tecniche più frequentemente utilizzate per studiare la dieta del lupo, dove il riconoscimento delle specie preda, nel caso dei mammiferi, si basa su caratteristiche microscopiche distintive del pelo. Tale tecnica, è tuttavia soggetta ad errori individuali nonostante l'esperienza dell'operatore e il ricorso a testi o collezioni di riferimento. Congiuntamente ad una mancata standardizzazione delle procedure di preparazione e trattamento del campione, queste fonti di errore possono alterare significativamente i risultati. Nell'ambito di un progetto UE-Life sull'ecologia del Lupo, in tre Parchi Regionali dell'Emilia-Romagna (2001-2004, abbiamo quindi applicato una procedura standardizzata per la selezione, raccolta, preparazione e trattamento dei campioni fecali di lupo ai fini dell'analisi della dieta. A tal fine è stato organizzato un corso di formazione da parte di docenti qualificati per un totale di 150 ore, suddiviso in moduli e articolato in esercitazioni e prove pratiche di autovalutazione. Al corso, e dopo un ulteriore periodo di esercitazioni individuali (ca. 300 ore/operatore, è quindi seguito un esame di identificazione che ha permesso di quantificare l'affidabilità di ciascun operatore. Il test per l'identificazione a livello di specie è stato basato su un campione di 120 peli, la cui specie di origine era ignota ai candidati (blind test, suddivisi tra ungulati selvatici (n=54: Capriolo, Cervo, Daino, Muflone, Cinghiale, ungulati domestici (n=21: bovini, ovini, caprini, equini, canidi (n= 18: lupo, cane, volpe e altre specie (n=27: lepre, marmotta, tasso, martora, faina, puzzola, gatto, scoiattolo, talpa, muridi. Un sottocampione di 65 peli è stato utilizzato per valutare l'affidabilità nel riconoscimento delle classi d'età (< 5 mesi, ≥ 5 mesi per le sole categorie degli ungulati selvatici (n=54 e degli

  2. Evidence-based Medicine e Clinical Governance: una survey tra giovani chirurghi italiani

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    Gabriele Maritati

    2012-05-01

    Full Text Available Background. La Clinical Governance (CG rappresenta in Italia la strategia di politica sanitaria di riferimento per la promozione di elevati standard qualitativi all’interno del SSN. Al centro della CG si colloca il movimento culturale dell’Evidence-based Medicine (EBM. Obiettivi. Misurare all’interno di un campione di giovani chirurghi: 1 la conoscenza dell’EBM e della CG 2 la rilevanza riconosciuta ai temi dell’EBM e della CG nella propria prospettiva professionale. Metodi. Un questionario anonimo è stato inviato via e-mail ai chirurghi iscritti alla Società Polispecialistica Italiana Giovani Chirurghi (SPIGC e a professionisti segnalati da altri colleghi. I chirurghi eleggibili dovevano essere specializzati da non oltre 4 anni o iscritti all’ultimo anno della Scuola di Specializzazione. Risultati. Sono stati invitati a partecipare 1077 chirurghi (1020 iscritti alla SPIGC e 57 segnalati. Dei 254 rispondenti (24% 137 erano eleggibili. Di questi, il 96% ha riferito di conoscere il termine Evidence-based Medicine e il 54 % quello di Clinical Governance. Il 62% ha dichiarato di conoscere la definizione dell’EBM e solo il 18% quello della CG. Per il 90% di essi, l’EBM condizionerà in maniera significativa la propria attività nei prossimi 15 anni; tale percentuale si riduce al 72% in merito alla CG. Inoltre, il 41% ritiene di non essere in grado di valutare critica mente la letteratura scientifica. Limiti. Il tasso di risposta alla survey potrebbe essere considerevolmente sottostimato poiché la mailing list della SPIGC comprende 1020 indirizzi non aggiornati in funzione dei soci correntemente iscritti che sono attualmente 435. Inoltre, i chirurghi partecipanti alla survey potrebbero non essere rappresentativi della totalità dei giovani chirurghi italiani, in quanto espressione di un sottogruppo particolarmente motivato alle tematiche dell’EBM e della CG. Conclusioni. Nonostante i giovani chirurghi italiani riconoscano la

  3. Valutazione del carico di stress nei Vigili del Fuoco: correlazione con il profilo psicologico ed alcuni indicatori biologici di rischio

    Directory of Open Access Journals (Sweden)

    P. Giacobazzi

    2003-05-01

    Full Text Available

    Obiettivo: approfondire i rapporti tra carico lavorativo, personalità ed alcuni indicatori biochimici di risposta allo stress le cui modificazioni possono contribuire a spiegare la suscettibilità/resistenza al rischio di malattie.

     Materiali e metodi: l’indagine è stata effettuata su un gruppo di Vigili del Fuoco (n°61 confrontati con un gruppo di controllo (ricercatori universitari; entrambi hanno aderito tramite consenso informato e fornito un campione di saliva per la misura del cortisolo e delle IgA in condizioni di base. Un sottogruppo di vigili ha fornito anche un campione di saliva dopo un turno lavorativo notturno (condizione di stress. Parallelamente si è effettuato un assessment psicologico tramite la somministrazione di questionari quali: lo State-Trait Anxiety Inventory, il Big Five Observer, il Locus of Control ed il Sensation Seeking Scale. Risultati: in condizioni basali i Vigili del Fuoco mostrano un livello di cortisolo salivare più elevato dei controlli (24,9 ± 11,3 vs 20,0 ± 8,6 nmol/l, p=0,091, mentre il livello di IgA è simile nei due gruppi. Nei vigili, dopo lo stress lavorativo, si è evidenziato un calo significativo delle IgA (296,5 ± 160,5 vs 191,9 ± 95,3 Bg/ml, p=0,014 ed una correlazione positiva tra il cortisolo salivare e l’ansietà di stato e di tratto.

    Conclusioni: i Vigili del Fuoco sono risultati meno ansiosi, più predisposti al rischio e con un profilo di personalità di tipo realistico in linea con la scelta professionale. Lo stress lavorativo si associa ad una riduzione significativa dell’ escrezione salivare di IgA, assunta come indice di attività del sistema immunitario, mentre il cortisolo salivare, che rappresenta un indicatore sensibile di attività neuroendocrina sotto stress, pur non modificandosi in termini assoluti si associa con il livello di ansietà dei vigili allo studio.

  4. Heavy metals determination in aquatic species for food purposes

    Energy Technology Data Exchange (ETDEWEB)

    Locatelli, C.; Torsi, G. [Bologna Univ., Bologna (Italy). Dept. of Chemistry G. Ciamician

    2001-02-01

    New analytical procedures and sample mineralizations are proposed regarding the determination of arsenic, selenium, copper, lead, cadmium, zinc and mercury in matrices involved in food chain as mussel, clams and fishes. As regards As, Se, Cu, Pb, Cd and Zn determinations. H{sub 2}SO{sub 4}-HNO{sub 3} acidic mixture is used for the digestion is performed using a concentrated supra pure H{sub 2}SO{sub 4}-K{sub 2}Cr{sub 2}O{sub 7} mixture and the results are compared with those from other conventional methods (DPASV) are employed for determining simultaneously selenium, arsenic and copper, lead, cadmium, zinc, respectively, while mercury determination is carried out by the cold vapour atomic absorption spectrometry (CV-AAS) with reduction with SnCl{sub 2}. The voltammetric measurements were performed using a conventional three-electrode cell and the ammonia-ammonium chloride buffer (ph 9.3) as supporting electrolyte. For all the elements, in addition to the detection limits, precision and accuracy data are also reported: the former, expressed as relative standard deviation (S{sub r}), and the latter, expressed as relative error (e), are in all cases between 3 to 6%. [Italian] Viene proposta una nuova procedura analitica ed una nuova mineralizzazione del campione per la determinazione di arsenico, selenio, rame, piombo, cadmio, zinco e mercurio in matrici coinvolte nella catena alimentare come mitili e pesci. Per la determinazione di As, Se, Cu, Pb, Cd, Zn, le matrici sono state mineralizzate con una miscela acida H{sub 2}SO{sub 4}-HNO{sub 3}. Nel caso del Hg, la mineralizzazione del campione e' stata effettuata mediante una miscela H{sub 2}SO{sub 4}-K{sub 2}Cr{sub 2}O{sub 7} ed i risultati confrontati con quelli ottenuti impiegando altri metodi convenzionali. Per la simultanea determinazione di arsenico, selenio, rame, piombo, cadmio, zinco, sono state impiegate la voltammetria differenziale pulsata catodica (DPCSV) ed anodica (DPASV), mentre il mercurio e

  5. L’uso delle risorse online per la ricerca delle informazioni cliniche da parte degli infermieri

    Directory of Open Access Journals (Sweden)

    Paola Ferri

    2012-06-01

    Full Text Available Background. Numerosi studi internazionali documentano che gli infermieri utilizzano risorse online per ricercare informazioni cliniche utili alla pratica quotidiana. I professionisti, tuttavia, dichiarano numerose difficoltà, tra cui la mancanza di tempo, le difficoltà di accesso alle biblioteche e ai loro servizi, le limitate competenze nella ricerca bibliografica. Obiettivi. Descrivere l’uso delle risorse online da parte degli infermieri italiani per ritrovare informazioni cliniche. Metodi. Studio trasversale condotto come survey attraverso l’invio di un questionario postale a un campione casuale di 520 infermieri da una popolazione di 977 infermieri di 2 dipartimenti affini (area critica appartenenti a 3 diversi ospedali della provincia di Modena. Risultati. Sono stati restituiti 298 questionari, pari al 57% del campione. Quasi il 90% degli infermieri dichiara di utilizzare Internet almeno una volta al giorno; solo il 60% può accedere alla rete sul posto di lavoro. Circa il 60% degli infermieri dichiara di usare solo i motori di ricerca come fonte di informazioni cliniche online e di non conoscere alcuna banca dati. MEDLINE è la banca dati più utilizzata dal 40% dei professionisti. Il 74% degli intervistati dichiara di utilizzare spesso le risorse online: da ogni giorno ad almeno 1 volta alla settimana. Le risorse online vengono utilizzate principalmente per la ricerca di informazioni necessarie a colmare gap di conoscenze cliniche e per mantenere aggiornati procedure, protocolli e istruzioni operative. Gli ostacoli maggiormente rilevati sono la mancanza di tempo, la limitata conoscenza della lingua inglese, difficoltà nell’utilizzo del computer e l’assenza di formazione specifica. Limiti. Percentuale modesta di questionari restituiti; popolazione in studio appartenente ad una sola azienda sanitaria; mancata verifica dell’attendibilità dello strumento, testato soltanto per la validità di contenuto. Conclusioni. Gli

  6. Informa(ction: How to do things with medicine information leaflets

    Directory of Open Access Journals (Sweden)

    Anna Vita Bianco

    2016-01-01

    Full Text Available Abstract – This paper analyses how Patient Information Leaflets (PILs of over-the-counter medical products convey information. More precisely, the article focuses on “instructions” on how to use medicines and related “instructional information” (Trimble 1985. The latter is used in PILs to explain why consumers should follow the instructions. The aim is to show, then, how and whether the PILs sampled help consumers to translate effectively the information contained into actions. The first part of the article examines the deontic devices employed in 8 English and Italian PILs of the most common over-the-counter medicines for human consumption used to relieve the same symptoms. The second part, instead, analyses whether and how the eight leaflets sampled followed the ‘instructions’ contained in one of the authoritative works by Sless and Shrensky in this field (2006. Both Australian authors, indeed, provide useful advice on how to write directives and related explanations in PILs.Riassunto – Questo lavoro si propone di analizzare come i foglietti illustrativi dei medicinali da banco veicolano le informazioni. Nel caso specifico, l’attenzione è rivolta alle “istruzioni” per l’uso (dei medicinali e alle “informazioni sulle istruzioni” (Trimble 1985. Quest’ultime vengono utilizzate nei foglietti illustrativi per spiegare ai consumatori perché seguire specifiche istruzioni. L’intento è quello di mostrare, quindi, come e se i foglietti illustrativi dei medicinali presi a campione aiutino i consumatori a tradurre in modo efficace le informazioni in azioni. La prima parte dell’articolo è incentrata sulle strategie deontiche utilizzate in 8 foglietti illustrativi di medicinali da banco inglesi e italiani per uso umano, usati comunemente per curare la stessa tipologia di sintomi. La seconda parte, invece, analizza se e come gli otto foglietti illustrativi presi a campione hanno seguito, a loro volta, le

  7. Die anderen Ausdrucksweisen: subtile Offensiven Different Modes of Expression: Subtle Offensives

    Directory of Open Access Journals (Sweden)

    Ella Jasiowka

    2002-03-01

    Full Text Available Was passiert, wenn der „Herrscher des filmischen Blickes“ eine Frau ist? Anhand zweier jeweils sehr unterschiedlicher Beispiele „weiblicher“ Filmkunst zeigt die Autorin, dass es nötig ist, herkömmliche Interpretationsmuster zu überwinden, um dem Filmschaffen einzelner Künstler und Künstlerinnen auf den Grund zu gehen. Da es nicht die „feministische Kunst“ an sich gibt, muß man für das Werk so unterschiedlicher Künstlerinnen wie Sally Potter und Jane Campion jeweils unterschiedliche Schlüssel suchen, um ihnen gerecht zu werden. Dies tut Radkiewicz in ihrem Werk – aus feministischer Perspektive. Sie holt jeweils verschieden weit aus, wenn es darum geht, künstlerische Herkunft, Biographie, Vorbilder und Ziele der Künstlerinnen zu deren Werk in Bezug zu setzten. Ohne zu bahnbrechend neuen Erkenntnissen zu kommen, gelingt es ihr doch, ein komplexes Bild der von ihr behandelten Filmemacherinnen und den Filmen zu entwerfen, das einen umfassenden Gesamteindruck vermittelt. Die Kürze des Buches bringt dabei eine Konzentration mit sich, die zu einer Weiterbeschäftigung und Vertiefung einlädt.What happens if the filmmaker directing the audience’s gaze is a woman? Radkiewicz uses two very different examples of “female” film art to portray the two directions into which this kind of undertaking can lead. In so doing, she demonstrates that traditional methods of analysis no longer suffice to thoroughly examine the work of an artist. Since there is no neatly defined category of “feminist art,” one has to take into consideration different kinds of approaches in order to do justice to the work of film directors like Jane Campion and Sally Potter. Radkiewicz does exactly that in her work, and thus provides an analysis that is informed by a feminist perspective. She includes the filmmakers’ artistic trajectory, background, biography, and goals into her interpretation of Campion’s and Potter’s films. While Radkiewicz does

  8. Rilevazione della percezione corporea, dell' autostima corporea e dei disturbi del comportamento alimentare in adolescenti vittime di abuso sessuale durante l' infanzia.

    Directory of Open Access Journals (Sweden)

    L. Bassoli

    2003-05-01

    Full Text Available

    Obiettivi: l’esperienza dell’abuso sessuale si ripercuote su diversi aspetti della personalità delle vittime. Il nostro studio ha analizzato la sua influenza sulla percezione corporea (Test delle figure di Collins, sull’autostima corporea (Scala Corporea del Test Multidimensionale Autostima di Bracken e sull’adozione di comportamenti alimentari patologici (Eating Attitude Test.

    Materiali e metodi: il campione della ricerca è composto da 2935 studenti maggiorenni frequentanti le 212 classi quinte dei 46 istituti superiori milanesi selezionati per la ricerca. Gli strumenti sono i test sopraindicati ed un questionario ad hoc per indagare le caratteristiche dell’eventuale abuso sessuale. La compilazione e la restituzione dei questionari hanno salvaguardato rigorosamente l’anonimato.

    Risultati: nel campione, l’esperienza dell’abuso incide sulla percezione corporea aumentando il divario tra l’immagine del corpo in cui ci si riconosce e l’immagine che si vorrebbe avere con una differenza media che nei maschi passa da 0,1 nei non abusati a –0,4 in vittime d’abuso lieve fino a 1 in vittime d’abuso grave, mentre nelle ragazze i valori sono rispettivamente 0,8, 0,9 e 1,3. La percentuale a rischio per disturbi del comportamento alimentare sale notevolmente tra le vittime d’abuso, essendo 1,5% e 11,8% in maschi e femmine non vittimizzati, 2,3% e 15,9% rispettivamente in vittime d’abuso lieve e 27,3% e 24,1% in vittime d’abuso grave. Anche sull’autostima corporea l’esperienza dell’abuso sembra avere un forte impatto che si manifesta con un aumento dei casi di bassa autostima che risultano il 15,6% e il 35,5% tra maschi e femmine non abusati, il 16% e il 41,2%, rispettivamente, tra chi ha subito un abuso lieve e il 27,3% e il 50% tra le vittime d’abuso grave.

    Conclusioni: la ricerca suggerisce che essere vittima abuso sessuale

  9. Appropriatezza della profilassi antibiotica perioperatoria in pediatria: un audit clinico

    Directory of Open Access Journals (Sweden)

    Klaus Peter Biermann

    2012-08-01

    Full Text Available Background. La disponibilità di numerose evidenze scientifiche in tema di profilassi antibiotica perioperatoria, insieme alla necessità di uniformare la pratica clinica e verificare la trasferibilità delle raccomandazioni in ambito pediatrico, hanno motivato la decisione di pianificare un ciclo di audit presso l’Azienda Ospedaliero-Universitaria Meyer di Firenze. Obiettivi. Valutare l’appropriatezza dell’antibiotico profilassi secondo le raccomandazione formulate dalle più autorevoli linee guida; identificare gli ostacoli all’implementazione delle raccomandazioni e definire gli interventi finalizzati alla loro rimozione, inclusi l’aggiornamento e la diffusione del protocollo interno all’ospedale, redatto nel 2005. Metodi. Un campione di 115 interventi chirurgici è stato analizzato rispetto alla somministrazione della profilassi antibiotica in chirurgia pediatrica. La pratica prescrittiva è stata confrontata con quanto raccomandato dalle linee guida, verificando l’appropriatezza della somministrazione antibiotica, il timing, il dosaggio in rapporto al peso, il ricorso alla seconda dose in relazione alla durata dell’intervento e la scelta della molecola. Risultati. Il tasso di appropriatezza della profilassi antibiotica è del 87.8% (101/115, con un’appropriatezza d’uso (ricorso alla somministrazione, quando raccomandata dell’81.4% (35/43 e un’appropriatezza del non uso (non somministrazione, quando non raccomandata del 91.7% (66/72. Tra i 41 soggetti sottoposti a profilassi, 12 risultano in pre-trattamento e pertanto la scelta della molecola è condizionata dal trattamento in corso al momento dell’intervento. Dei rimanenti 29, il 75.9% sono sottoposti a profilassi con cefalosporine di III generazione, non raccomandate dalle linee guida. Limiti. Lo studio è stato condotto su un campione di 115 cartelle cliniche, pari a poco più del 3% dei dimessi nei primi sette mesi del 2011. La scelta dei criteri di

  10. Modelli organizzativi per la gestione della Day surgery in ambito pediatrico (DSP

    Directory of Open Access Journals (Sweden)

    T. Langiano

    2003-05-01

    Full Text Available

    Obiettivi: nell’ambito delle iniziative avviate negli ultimi anni a livello nazionale e regionale volte al recupero di appropriatezza nell’erogazione di prestazioni sanitarie mediante la deospedalizzazione dei pazienti, si inserisce l’indicazione del PO Materno-infantile 1998-2000, tra gli obiettivi relativi alla assistenza al bambino in ospedale, “all’applicazione di protocolli mirati a ridurre i ricoveri impropri attraverso l’implementazione del DH…”. Al fine di valutare la situazione attualmente esistente in tema di DSP e di promuovere una omogeneizzazione, mediante la definizione e la sperimentazione di un protocollo operativo specifico, il Ministero della Salute ha affidato all’OPBG il coordinamento del Progetto “Sviluppo dei criteri e modelli di riferimento per la diffusione della day surgery in campo pediatrico”. Nell’ambito del Progetto è stato effettuato un censimento dei modelli di DSP esistenti sul territorio nazionale.

    Metodologia: è stato individuato un campione di 108 ospedali che, nel 2000, hanno dimesso almeno 500 pazienti di età inferiore a 18 anni attribuiti a DRG chirurgici e che rendono conto del 52,2% dell’attività svolta in chirurgia pediatrica sul territorio nazionale. La scheda di rilevazione predisposta conteneva domande finalizzate a individuare le principali soluzioni organizzative adottate per l’erogazione di ricoveri in DSP e a delineare la casistica trattata.

    Risultati: alla rilevazione (settembre 2002-gennaio 2003 hanno partecipato 88 ospedali (81,5% del campione, di cui 49 effettuano prestazioni in DSP. I risultati indicano una discreta variabilità dei modelli organizzativi della DSP e una elevata concentrazione della casistica trattata: i 10 DRG più frequentemente trattati spiegano oltre l’80% dei casi di DSP.

    Conclusioni: i risultati ottenuti dimostrano come in Italia esistano ancora ampi

  11. Phosphorus utilization and microbial community in response to lead/iron addition to a waterlogged soil

    Institute of Scientific and Technical Information of China (English)

    ZHONG Shunqing; WU Yuping; XU Jianming

    2009-01-01

    Constructed wetlands have emerged as a viable option for helping to solve a wide range of water quality problems. However, heavy metals adsorbed by substrates would decrease the growth of plants, impair the functions of wetlands and eventually result in failure of contaminant removal. Typha latifolia L., tolerant to heavy metals, has been widely used for phytoremediation of Pb/Zn mine tailings under waterlogged conditions. This study examined effects of iron as ferrous sulfate (100 and 500 mg/kg) and lead as lead nitrate (0, 100, 500 and 1000 mg/kg) on phosphorus utilization and microbial community structure in a constructed wetland. Wetland plants (T. latifolia) were grown for 8 weeks in rhizobags filled with a paddy soil under waterlogged conditions. The results showed that both the amount of iron plaque on the roots and phosphorus adsorbed on the plaque decreased with the amount of lead addition. When the ratio of added iron to lead was 1:1, phosphorus utilized by plants was the maximum. Total amount of phospholipids fatty acids (PLFAs) was 23%-59% higher in the rhizosphere soil than in bulk soil. The relative abundance of Gram-negative bacteria, aerobic bacteria, and methane oxidizing bacteria was also higher in the rhizosphere soil than in bulk soil, but opposite was observed for other bacteria and fungi. Based on cluster analysis, microbial communities were mostly controlled by the addition of ferrous sulfate and lead nitrate in rhizosphere and bulk soil, respectively.

  12. Effect of ecological factors on the zonation of wetland vegetation

    Directory of Open Access Journals (Sweden)

    Richard Hrivnák

    2011-04-01

    Full Text Available The influence of some ecological factors to aquatic and marsh vegetation was studied during 1998-2000. Three basic vegetation units (Caricetum buekii, Typhetum latifoliae and Ceratophylletum submersi and three transitional communities were defined in the belt transect, which was established along the moisture gradient. The content of available soil nutrients in individual vegetation types differed only in case of the Ceratophyllum submersum community, where a higher magnesium and nitrogen content accumulated due to specific environmental conditions. Water and marsh vegetation is usually characterised by a pronounced spatial and temporal dynamics. In the studied area, its zonation was dependent from the terrain morphology, and both depth and duration of floods. The fluctuation of ground and surface water table during a three-year period caused changes in the occurrence and cover of several species (e.g. Carex buekii, Typha latifolia, aquatic macrophytes. Pronounced changes in the cover of some species occurred even within a single vegetation season due to the long-term sink of water table below the ground surface.

  13. Screening of 18 species for digestate phytodepuration.

    Science.gov (United States)

    Pavan, Francesca; Breschigliaro, Simone; Borin, Maurizio

    2015-02-01

    This experiment assesses the aptitude of 18 species in treating the digestate liquid fraction (DLF) in a floating wetland treatment system. The pilot system was created in NE Italy in 2010 and consists of a surface-flow system with 180 floating elements (Tech-IA®) vegetated with ten halophytes and eight other wetland species. The species were transplanted in July 2011 in basins filled with different proportions of DLF/water (DLF/w); periodic increasing of the DLF/w ratio was imposed after transplanting, reaching the worst conditions for plants in summer 2012 (highest EC value 7.3 mS cm/L and NH4-N content 225 mg/L). It emerged that only Cynodon dactylon, Typha latifolia, Elytrigia atherica, Halimione portulacoides, Salicornia fruticosa, Artemisia caerulescens, Spartina maritima and Puccinellia palustris were able to survive under the system conditions. Halophytes showed higher dry matter production than other plants. The best root development (up to 40-cm depth) was recorded for Calamagrostis epigejos, Phragmites australis, T. latifolia and Juncus maritimus. The highest nitrogen (10-15 g/m(2)) and phosphorus (1-4 g/m(2)) uptakes were obtained with P. palustris, Iris pseudacorus and Aster tripolium. In conclusion, two halophytes, P. palustris and E. atherica, present the highest potential to be used to treat DLF in floating wetlands.

  14. Water level, vegetation composition and plant productivity explain greenhouse gas fluxes in temperate cutover fens after inundation

    Directory of Open Access Journals (Sweden)

    M. Minke

    2015-10-01

    Full Text Available Rewetting of temperate continental cutover peatlands generally implies the creation of flooded areas, which are – dependent on water depth – colonized by helophytes such as Eriophorum angustifolium, Carex spp., Typha latifolia or Phragmites australis. Reeds of Typha and Phragmites are reported to be large sources of methane, but data on net CO2 uptake are contradictory for Typha and rare for Phragmites. This paper describes the effect of vegetation, water level and nutrient conditions on greenhouse gas (GHG emissions for representative vegetation types along water level gradients at two rewetted cutover fens (mesotrophic and eutrophic in Belarus. Greenhouse emissions were measured with manual chambers in weekly to few – weekly intervals over a two years period and interpolated by modelling. All sites had negligible nitrous oxide exchange rates. Most sites were carbon sinks and small GHG sources. Methane emissions were generally associated with net ecosystem CO2 uptake. Small sedges were minor methane emitters and net CO2 sinks, while Phragmites australis sites released large amounts of methane and sequestered very much CO2. Variability of both fluxes increased with site productivity. Floating mats composed of Carex tussocks and Typha latifolia were a source for both methane and CO2. We conclude that shallow, stable flooding is a better measure to arrive at low GHG emissions than deep flooding, and that the risk of high GHG emissions consequent on rewetting is larger for eutrophic than for mesotrophic peatlands.

  15. Novos registros de espécies da subtribo Ecliptinae (Heliantheae - Asteraceae para a Amazônia brasileira New records of species of the Ecliptinae subtribe (Heliantheae - Asteraceae to the Brazilian Amazon

    Directory of Open Access Journals (Sweden)

    Genilson Alves dos Reis e Silva

    2010-09-01

    Full Text Available Sete espécies da subtribo Ecliptinae encontradas nos estados do Acre, Amapá, Amazonas, Pará e Rondônia, são apresentadas como novos registros para a Amazônia brasileira: Acmella uliginosa, Aspilia camporum, Aspilia ulei, Melanthera latifolia, Melanthera nivea, Spilanthes nervosa e Wedelia calycina. São apresentadas descrições e ilustrações para as espécies, dados sobre a distribuição geográfica, hábitat, época de floração e frutificação. Os novos registros evidenciam a importância de estudos sobre a flora amazônica e demonstram a necessidade de coletas mais intensas na região.The following seven species of the subtribe Ecliptinae found in the states of Acre, Amapá, Amazonas, Pará and Rondônia have been recorded at the Brazilian Amazonia, for the first time: Acmella uliginosa, Aspilia camporum, Aspilia ulei, Melanthera latifolia, Melanthera nivea, Spilanthes nervosa and Wedelia calycina. Species descriptions and illustrations are presented, as well as information about geographic distribution, habitats and phenology. These new records highlight the importance of the floristic studies in Amazonia, and the need to carry out intensive fieldwork to improve the sampling in this region.

  16. Functional linkage between N acquisition strategies and aeration capacities of hydrophytes for efficient oxygen consumption in roots.

    Science.gov (United States)

    Nakamura, Motoka; Nakamura, Takatoshi; Tsuchiya, Takayoshi; Noguchi, Ko

    2013-02-01

    We evaluated the specific strategies of hydrophytes for root O(2) consumption in relation to N acquisition and investigated whether the strategies varied depending on the aeration capacity. Aeration capacity of roots is an important factor for determining hypoxia tolerance in plants. However, some hydrophytes possessing quite different aeration capacities often co-occur in wetlands, suggesting that root O(2) consumption also strongly affects hypoxia tolerance. We cultivated Phragmites australis with high aeration capacity and Zizania latifolia with low aeration capacity in hypoxic conditions with NH(4)(+) or NO(3)(-) treatment and compared the growth, N uptake, N assimilation and root respiration between the two species. In Z. latifolia grown with NH(4)(+) treatment, high N uptake activity and restrained root growth led to sufficient N acquisition and decrease in whole-root respiration rate. These characteristics consequently compensated for the low aeration capacity. In contrast, in P. australis, low N uptake activity was compensated by active root growth, but the whole-root respiration rate was high. This high root respiration rate was allowed by the high aeration capacity. The O(2) consumption-related traits of hydrophyte roots were closely correlated with N acquisition strategies, which consequently led to a compensational relationship with the root aeration capacity. It is likely that this functional linkage plays an important role as a core mechanism in the adaptation of plants to hypoxic soils.

  17. Polyploid evolution and Pleistocene glacial cycles: A case study from the alpine primrose Primula marginata (Primulaceae

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    Casazza Gabriele

    2012-04-01

    Full Text Available Abstract Background Recent studies highlighted the role of Pleistocene climatic cycles in polyploid speciation and of southern Alpine refugia as reservoirs of diversity during glacial maxima. The polyploid Primula marginata, endemic to the southwestern Alps, includes both hexaploid and dodecaploid cytotypes that show no ecological or morphological differences. We used flow cytometry to determine variation and geographic distribution of cytotypes within and between populations and analyses of chloroplast (cp and nuclear ribosomal (nr DNA sequences from the Internal Transcribed Spacer (ITS region to infer the evolutionary history of the two cytotypes and the auto- vs. allopolyploid origin of dodecaploid populations. Results We did not detect any intermediate cytotypes or variation of ploidy levels within populations. Hexaploids occur in the western and dodecaploids in the eastern part of the distributional range, respectively. The cpDNA and nrDNA topologies are in conflict, for the former supports shared ancestry between P. marginata and P. latifolia, while the latter implies common origins between at least some ITS clones of P. marginata and P. allionii. Conclusions Our results suggest an initial episode of chloroplast capture involving ancestral lineages of P. latifolia and P. marginata, followed by polyploidization between P. marginata-like and P. allionii-like lineages in a southern refugium of the Maritime Alps. The higher proportion of ITS polymorphisms in dodecaploid than in hexaploid accessions of P. marginata and higher total nucleotide diversity of ITS clones in dodecaploid vs. hexaploid individuals sequences are congruent with the allopolyploid hypothesis of dodecaploid origin.

  18. Ecological aspects of selenium effects on plant growth and species diversity in soils with elevated concentrations of salinity and selenium

    Energy Technology Data Exchange (ETDEWEB)

    Huang, Zhangzhi.

    1991-01-01

    A field study was conducted in soils with elevated concentrations of salinity and selenium during 1986-1990 at Kesterson Reservoir, Merced County, California. The investigation was conducted in three stages of plant habitat restoration: (1) wet habitat, (2) dry habitat, and (3) fill dirt cover habitat. The total water extractable selenium concentrations of wet habitat, dry habitat and fill dirt cover habitat were 2260-3700, 90-670, and undetectable-37 [mu]g/kg dry soil, respectively. Among the vascular flowering plants, saltgrass (Distichlis spicata L.) was the dominant species in dry habitat, and cattail (Typha latifolia L.) was the dominant species in wet habitat in the evaporation ponds at Kesterson. High concentrations of selenium were found in Kesterson marsh plant species. In wet habitat, selenium concentrations averaged 12.50 ppm ([mu]g/g dry wt) in Distichlis spicata leaves, 15.20 ppm in Typha latifolia leaves and 4.10 ppm in Juncus mexicanus leaves, respectively. In dry habitat, the tissue selenium concentration was about 1.5 ppm for Distichlis spicata and 4 ppm for Atriplex species. In fill dirt cover habitat, plant tissue selenium concentrations ranged from 1 to 19 ppm. Biomass distribution, species richness, and selenium accumulation of plants were studied for four sites during 1988-1990. At two sites, the surface soil consisted of fill dirt. Another two sites were native-soil cover (including Kesterson sediment).

  19. Effect of cyclic phytoremediation with different wetland plants on municipal wastewater.

    Science.gov (United States)

    Farid, Mudassar; Irshad, Muhammad; Fawad, Muhammad; Ali, Zeshan; Eneji, A Egrinya; Aurangzeb, Naureen; Mohammad, Ashiq; Ali, Barkat

    2014-01-01

    Phytoremediation is a promising cleanup technology for contaminated soils, groundwater, and wastewater that is both low-tech and low-cost. The objective of this study was to investigate the ameliorative effect of phytoremediation on municipal wastewater (MWW). For this purpose, a phytoremediation garden was established using different aquatic plants species [Pistia stratiotes, Eichhornia crassipess, Hydrocotyle umbellatta, Lemna minor, Tyhpa latifolia, and Scirpus acutus] in seven earthen pond systems (P1-P7) for the cyclic treatment of MWW. The physico-chemical analysis of MWW was carried out before and after the cyclic phytoremediation. Results showed that pH, EC and turbidity of MWW were reduced by 5.5%, 33.7%, and 93.1%, respectively after treatment (from P1 to P7). Treatment system also reduced total dissolved solids (TDS) by 35.2%, Cl by 61%, HCO3 by 29.2%, hardness by 45.7%, Ca by 32.3% and Mg by 55.9%. Nitrate concentration was reduced by 77.6% but SO4 was enhanced slightly. An ameliorative combined effect of wetland plants namely L. minor, T. latifolia, and S. acutus on MWW was noticed. Sequential phytoremediation with a mixture of plants was more effective than that relying only on a single plant species. PMID:24912243

  20. Producción de frutos y semillas de dos especies arbóreas nativas en un bosque mesófilo de montaña de Veracruz, México

    Directory of Open Access Journals (Sweden)

    Yureli García-De La Cruz

    2015-07-01

    Full Text Available Se estimó y comparó la producción de frutos y semillas de una muestra de árboles pertenecientes a Alchornea latifolia y Liquidambar styraciflua en un bosque de niebla en la zona centro del estado de Veracruz. Los individuos se seleccionaron con base en sus características fenotípicas; se tomaron datos estructurales (diámetro a la altura del pecho, altura y cobertura y éstas se compararon con la producción semillera en cada especie. Se estimó una producción de 70 380 frutos, 140 760 semillas y 6.02 kg por árbol en Alchornea latifolia y, 5 738 frutos, 303 218 semillas y 0.7 kg por árbol en Liquidambar styraciflua. Ninguna de las variables estructurales de los árboles superiores contribuyó a explicar la producción de frutos y semillas. Sin embargo, la variación entre individuos (identidad del árbol contribuyó a explicar las diferencias observadas en la producción de infrutescencias por rama, el número de semillas por infrutescencia, así como el peso de las semillas por infrutescencia en ambas especies.

  1. Nuevas citas de Asteraceae para la provincia de Catamarca (Argentina New records of Asteraceae for the province of Catamarca (Argentina

    Directory of Open Access Journals (Sweden)

    Susana E. Freire

    2011-06-01

    Full Text Available Se mencionan los siguientes nuevos registros de Asteráceas para la provincia de Catamarca, Argentina: Baccharis acaulis, B. boliviensis var. latifolia, B. flexuosa, B. glutinosa var. angustissima, B. stenophylla (Tribu Astereae, Eupatorium patens var. tomentosum (Tribu Eupatorieae, Cosmos bipinnatus, C. peucedanifolius var. peucedanifolius, Helianthus annuus (Tribu Heliantheae, Gamochaeta erythractis, G. longipedicellata, Gnaphalium lacteum, Tessaria integrifolia var. integrifolia (Tribu Inuleae, Chuquiraga ruscifolia (Tribu Mutisieae, Senecio crepidifolius, S. subulatus var. subulatus, Xenophyllum incisum var. incisum (Tribu Senecioneae. Se citan además el hábitat de los taxones y las provincias fitogeográficas que integran. Se propone una nueva combinación, Baccharis glutinosa Pers. var. angustissima (DC. Giuliano.The following Asteraceae are mentioned for the province of Catamarca: Baccharis acaulis, B. boliviensis var. latifolia, B. flexuosa, B. glutinosa var. angustissima, B. stenophylla (Tribe Astereae, Eupatorium patens var. tomentosum (Tribe Eupatorieae, Cosmos bipinnatus, C. peucedanifolius var. peucedanifolius, Helianthus annuus (Tribe Heliantheae, Gamochaeta erythractis, G. longipedicellata, Gnaphalium lacteum, Tessaria integrifolia var. integrifolia (Tribe Inuleae, Chuquiraga ruscifolia (Tribe Mutisieae, Senecio crepidifolius, S. subulatus var. subulatus, Xenophyllum incisum var. incisum (Tribe Senecioneae. The habitat and phytogeographic provinces of the taxa are mentioned. The following new combination is proposed: Baccharis glutinosa Pers. var. angustissima (DC. Giuliano.

  2. Influência do Cultivo Agrícola Convencional nas Características Químicas e Macrofauna Edáfica

    Directory of Open Access Journals (Sweden)

    Lécio Resende Pereira Júnior

    2010-10-01

    Full Text Available With the increasing population, the exploitation of natural resources, increasingly, has intensified. However, research which highlight the relationship between the production system and environmental sustainability are still incipient. In this sense, this study aimed to evaluate the influence of conventional cultivation, chemical characteristics and edafic macrofauna. The research was performed in the cities of Areia and Remigio, PB, from August to September/2009. Five sites were selected, cultivated Psidium guajava, Mimosa caesalpiniifolia, Citrus latifolia, the consortium Annona muricata x Citrus latifolia and a forest area. It was installed a total of 25 traps Provid and the soil sampled. Later the traps were collected and identified the macrofauna taxonomic level of Order. The experimental design was completely randomized. It was found 370.0 individuals captured in eight different Orders, with a predominance of the Orders Hymenoptera, Coleoptera and Araneae. There was a decrease in organic matter content, CTC and an increase in the levels of soil nutrients. It was concluded that the organic and mineral fertilization, combined with the reduced management of the soil result in reduced impacts on the diversity of soil organisms and the human disturbance of areas used for agriculture impairs negatively the edaphic ecosystem dynamics

  3. Screening of 18 species for digestate phytodepuration.

    Science.gov (United States)

    Pavan, Francesca; Breschigliaro, Simone; Borin, Maurizio

    2015-02-01

    This experiment assesses the aptitude of 18 species in treating the digestate liquid fraction (DLF) in a floating wetland treatment system. The pilot system was created in NE Italy in 2010 and consists of a surface-flow system with 180 floating elements (Tech-IA®) vegetated with ten halophytes and eight other wetland species. The species were transplanted in July 2011 in basins filled with different proportions of DLF/water (DLF/w); periodic increasing of the DLF/w ratio was imposed after transplanting, reaching the worst conditions for plants in summer 2012 (highest EC value 7.3 mS cm/L and NH4-N content 225 mg/L). It emerged that only Cynodon dactylon, Typha latifolia, Elytrigia atherica, Halimione portulacoides, Salicornia fruticosa, Artemisia caerulescens, Spartina maritima and Puccinellia palustris were able to survive under the system conditions. Halophytes showed higher dry matter production than other plants. The best root development (up to 40-cm depth) was recorded for Calamagrostis epigejos, Phragmites australis, T. latifolia and Juncus maritimus. The highest nitrogen (10-15 g/m(2)) and phosphorus (1-4 g/m(2)) uptakes were obtained with P. palustris, Iris pseudacorus and Aster tripolium. In conclusion, two halophytes, P. palustris and E. atherica, present the highest potential to be used to treat DLF in floating wetlands. PMID:25005162

  4. 复方氯己定联合苦丁茶对呼吸机相关性肺炎口腔的护理效果%Effect of the compound of chlorhexidine and"Kuding"tea on the oral hygiene and prevention of ventilator -associated pneumonia

    Institute of Scientific and Technical Information of China (English)

    李磊

    2014-01-01

    目的:探讨复方氯己定联合苦丁茶对机械通气患者预防呼吸机相关性肺炎口腔的护理效果。方法选取机械通气患者162例,随机分为A组、B组、C组。 A组患者采用复方氯己定擦洗联合苦丁茶冲洗,B组患者采用复方氯己定擦洗,C组患者采用苦丁茶冲洗。连续7d观察三组患者口腔霉菌、口臭、口腔溃疡、咽部和气管内细菌定植数、VAP的发生率,不良反应、口腔护理效果。结果口咽部细菌定植发生率A组患者明显低于B组(P<0.05),A组和B组均明显低于C组(均P<0.05);VAP发生率A组患者明显低于B组( P<0.05),A组和B组均明显低于C组(均P<0.05);牙齿、舌苔发黄等发生率A组和C组均明显低于B组(均P<0.05),A组患者明显低于C组(P<0.05)。结论采用复方氯己定冲洗、擦洗联合苦丁茶液冲洗对VAP患者进行口腔护理可以起到很好地抗菌消炎作用,降低VAP的发生率。%Objective To study the clinical effect of the compound comprising chlorhexidine and "Kuding"tea ( Folium Ilicis Latifoliae) in preventing patients who are connected to ventilator machines from contracting ventilator -associated pneumonia ( VAP) .Methods A total of 162 patients who were on ventilator machines were divided randomly into three groups , A, B, and C.Group A used a compound of chlorhexidine and "Kuding"tea ( Folium Ilicis Latifoliae ) for oral washing; Group B used chlorhexidine for oral washing;Group C used"Kuding"tea ( Folium Ilicis Latifoliae ) for oral washing.The effect on the oral hygiene of the patients were observed.Results The incidence of bacterial colonization in the pharynx oralis and trachea , VAP, and adverse effect of the oral cavity in group A patients were lower than that of group B and group C.Conclusions Using the compound of chlorhexidine and "Kuding"tea ( Folium Ilicis Latifoli-ae) for oral washing is better than using chlorhexidine or "Kuding

  5. State of weed infestation and features of sugar beet protection in Belarus

    Directory of Open Access Journals (Sweden)

    Soroka Sergey Vladimirovich

    2006-01-01

    Full Text Available The changes of phytosanitary situation recently taking place in sugar beet crops in the Republic of Belarus are shown. It is noticed that in the crop agrocoenosises there is a high infestation level caused by Japanese barnyard millet (Echinochloa crus-galli (L Pal. Beauv, field sowthistle (Sonchus arvensis L, chickweed (Stellaria media (L Vill, quick grass (Agropyron repens (L Pal Beauv, matricary (Matricaria perforate Merat, creeping thistle (Circium arvense (L scop, marsh woundwort (Stachus palustris L wild buckwheat (Polygonum convolvulus L, bristle stem hemp nettle (Galeopsis tetrahit L, common horsetail (Equisetum arvense L, field forget-me-not (Myosotis arvensis (L Hill, shepherd's purse (Capsella bursa-pastoris (L Med etc. Due to non-observance of preventive and separate agrotechnical techniques especially in spring-summer period, such weeds as bedstraw (Galium aparine L, white campion (Melandrium album (Mill Garcke, green amaranthus (Amaranthus retroflexus L started to appear in the crops. To protect sugar beet effectively, two variants of herbicides application are proposed. The first one - a combined, one stipulating soil action herbicides application before planting or before sugar beet seedlings emergence and on seedlings - to carry out two treatment by post-emergence preparations. The second variant, a split post- -emergence herbicide application (two-three times spraying on growing weeds at small application rates. In the next 5-6 years, a combined method will be of a primary importance in the conditions of the Republic.

  6. Tolerance or Assimilation: The Legends of the Chinese Restaurant and "The Gypsy's Tavern"

    Directory of Open Access Journals (Sweden)

    Ivan Kovačević

    2016-02-01

    Full Text Available Studying urban legends, the French folklorist Véronique Campion-Vincent posed the question of whether some of the more recent legends preach tolerance. The "elevator incident" or "swallowed ticket" legends display a different attitude to Others from that found in classic xenophobic urban legends. This different attitude is also to be found in two legends recorded in Serbia, namely, the legend of the Chinese restaurant and the legend of "The Gypsy's Tavern". An analysis of the two legends shows that the ambiguity of "tolerance legends" does not arise from the fact that they speak about a xenophobic environment while at the same time having a denouement that "preaches" tolerance, but rather from the fact that the "preaching" relates to those Others who have gone through a process of acculturation, who have been assimilated and who have accepted the rules of "our" culture. These legends do not preach tolerance towards the Otherness of Others but towards Others who are striving to become or have managed to become "Us".

  7. Preliminary study for the determination of heavy metal in ground samples by GF-ASS Zeeman; Studio preliminare per la determinazione di metalli pesanti in campioni di suolo mediante analisi GF-AAS Zeeman

    Energy Technology Data Exchange (ETDEWEB)

    Casabianca, T.; Bitonte, R.; Epifani, M.; Ubaldi, C. [ENEA, Divisione Tecnologie Ingegneria e Servizi Ambientali, Centro Ricerche Trisaia, MT (Italy)

    2001-07-01

    In the framework of SIMOA project have been investigated methods to evaluate the level of soil contamination due to heavy metals. In this wok, it is discussed a procedure to measure topsoil bioavailable fraction of seven heavy metals (Cd, Cu, Pb, Ni, Cr, Hg). The adopted procedure is based on acid digestion followed by instrumental detection by means of graphite furnace atomic-absorption spectrophotometry (GFAAS) using Zeeman effect to reduce background contribution. Details of samples preparation and analysis, experimental setup optimization and statistical data analysis are presented, together with a discussion on method accuracy and data interpretation. [Italian] Nell'ambito del progetto SIMOA (Sistema Integrato di Monitoraggio Ambientale) per il monitoraggio ambientale nel bacino del Basento (Regione Basilicata, Italia), vengono investigati i metodi per il controllo dei livelli di inquinamento del suolo da parte di metalli pesanti. Nel presente lavoro viene proposta una procedura per determinare il livello di concentrazione della frazione biodisponibile di sette metalli pesanti (Cadmio, Rame, Piombo, Nickel, Cromo, Mercurio) in campion di suolo superficiale. Il metodo e' basato su di un trattamento di digestione acida in forno a microonde cui segue la rivelazione strumentale mediante spettrofotometria di assorbimento atomico in fornetto di grafite (GFAAS) con effetto Zeeman per la correzione del fondo. Si descrivono in dettaglio le fasi di preparazione dei campioni, la metodologia di misura e l'analisi statistica dei dati, oltre ad una discussione sull'attendibilita' del metodo e sui futuri sviluppi.

  8. Gender Is Cultural Rather Than Natural--Jane Campion’s The Piano

    Institute of Scientific and Technical Information of China (English)

    李懿

    2014-01-01

    Sex and gender have been confusingly categorised by many scholars. Sex refers to biological characteristics;gender, can also be influenced by biology but does not determined by it. Unlike sex, which is determined by nature at birth, gender is a social construct specifying the culturally prescribed roles that men and women are to follow and represent. In order to argue that gender attaches to culture and that gender identity is shaped socially and culturally, this essay analyses Jane Campion ’s The Piano from three perspectives. Firstly, the essay will examine the way people in the film represent their gender characters through three re-peatedly demonstrated narrative motifs, clothing, the axe and the piano, to illustrate that gender is performative and that cultural gender identities advocate specific performances in terms of behaviour, speech, views, etc. (Butler, 2011). Then, through an analy-sis of the different gazes of the characters in the film, the essay indicates that social and cultural interaction between the genders can go beyond and blur the boundaries of the stereotypical categories of male and female. In the third section, the essay will argue that social rules and discourse also play a prominent role in shaping people’s gender, by discussing how the characters represent and re-define their gender identity under the pressure of the patriarchal society they inhabit.

  9. La vulnerabilità finanziaria: un’analisi per classi di reddito

    Directory of Open Access Journals (Sweden)

    Paolo Acciari

    2014-12-01

    Full Text Available In questo lavoro, mediante l’utilizzo di un ampio campione di individui tra i 25 e i 54 anni, si analizza la partecipazione al mercato del credito e la vulnerabilità finanziaria per classe di reddito tra il 2005 e il 2011. Nel periodo considerato la percentuale di individui che detenevano un debito di almeno 75 mila euro è aumentata, prevalentemente per il rallentamento del ritmo di rimborso del debito. La dinamica è stata eterogenea tra le classi di reddito: i nuovi indebitati appartenevano prevalentemente ai decili di reddito più elevati; il rallentamento nel ritmo di rimborso è stato più accentuato per le fasce di reddito medie e basse. Nel periodo sono aumentate le difficoltà nel pagamento del debito: i tassi di anomalia nel quintile più povero della popolazione erano 10 volte più elevati di quelli nel quintile più ricco; tale divario si è ampliato con la crisi. Nostre stime mostrano che l’instabilità del reddito accresce significativamente l’insorgenza di difficoltà finanziarie. Tuttavia, l’elevata concentrazione del debito presso le classi di reddito più ricche ha contribuito a contenere la rischiosità complessiva.

  10. [Funzioni esecutive metacognitive ed emozionali/motivazionali nel disturbo dello spettro dell'autismo e nel disturbo da deficit di attenzione con iperattività: risultati preliminari].

    Science.gov (United States)

    Panerai, Simonetta; Tasca, Domenica; Ferri, Raffaele; Catania, Valentina; Genitori D'Arrigo, Valentina; Di Giorgio, Rosa; Zingale, Marinella; Trubia, Grazia; Torrisi, Anna; Elia, Maurizio

    2016-01-01

    RIASSUNTO. Scopo. I deficit delle funzioni esecutive (FE) sono frequentemente osservati nei disturbi dello spettro dell'autismo (ASD) e nel disturbo da deficit di attenzione con iperattività (ADHD). Lo scopo di questo studio è quello di valutare e confrontare le funzioni esecutive metacognitive ed emozionali/motivazionali di bambini con ASD e ADHD, sia fra di loro che con un gruppo di controllo. Metodi. Il campione è costituito da un totale di 58 soggetti, di cui 17 con ASD senza disabilità intellettiva, 18 con ADHD-manifestazione combinata e 23 con sviluppo tipico, abbinati per genere, età cronologica e livello intellettivo. Le valutazioni hanno riguardato alcune aree del funzionamento esecutivo, nello specifico pianificazione, flessibilità mentale, generatività e inibizione della risposta, che rappresentano sia le funzioni esecutive metacognitive sia quelle emozionali/motivazionali. Risultati. I risultati hanno rilevato un'ampia sovrapposizione delle disfunzioni esecutive nei due gruppi clinici con ASD e ADHD, e non sono stati indicativi della presenza di due profili realmente distinti del funzionamento esecutivo. Tuttavia, nell'ADHD è stato trovato un deficit più severo nell'inibizione della risposta prepotente (funzione emozionale/motivazionale). Conclusioni. I risultati del nostro studio sono parzialmente in accordo con quelli della letteratura. Ulteriori ricerche con gruppi più numerosi potranno chiarire più approfonditamente quali punti di forza e debolezza, nell'ampio spettro delle funzioni esecutive, differenzino fra loro le prestazioni di persone con ASD e ADHD.

  11. Flow sorting of the Y sex chromosome in the dioecious plant Melandrium album

    Energy Technology Data Exchange (ETDEWEB)

    Veuskens, J.; Jacobs, M.; Negrutiu, I. [Free Univ. of Brussels (Belgium)] [and others

    1995-12-01

    The preparation of stable chromosome suspensions and flow cytometric sorting of both the Y sex chromosome of the white campion, Melandrium album, and the deleted Y chromosome of an asexual mutant, 5K63, is described. The principle has been to maintain transformed roots in vitro, synchronize and block mitosis, reduce cells to protoplasts, and lyse these to release chromosomes. Such in vitro material, unlike many cell suspensions, showed a stable karyotype. Factors critical to producing high-quality chromosome suspensions from protoplasts include osmolality of isolation solutions and choice of spindle toxin and of lysis buffer. Agrobacterium rhizogenes transformed young growing root cultures were synchronized at G1/S with 50 {mu}M aphidicolin for 24 h and released to a mitotic block with 30 {mu}M oryzalin for 11 h. Protoplast preparations from such tissue routinely had metaphase indices reaching 15%. Suspensions of intact metaphase chromosomes, with few chromatids, were obtained by lysing swollen mitotic protoplasts in a citric acid/disodium phosphate buffer. Except for the presence of clumps of autosomal chromosomes near the X and Y chromosome zones, monoparametric histograms of fluorescence intensities of suspensions stained with 4{prime},6-diamidino-2-phenylindole showed profiles similar to theoretical flow karyotypes. Two types of Y chromosomes, one full-length and one partially deleted (from the asexual mutant), could be sorted at 90% purity (21-fold enrichment of Y). These results are discussed in the context of sex determination and differentiation in higher plants. 45 refs., 6 figs., 2 tabs.

  12. An analytical method for determination of mercury by cold vapor atomic absorption spectroscopy; Determinazione di mercurio. Metodo per spettrometria di assorbimento atomico a vapori freddi (CV-AAS)

    Energy Technology Data Exchange (ETDEWEB)

    Campanella, L. [Rome Univ. La Sapienza, Rome (Italy); Mastroianni, D.; Capri, S.; Pettine, M. [CNR, Rome (Italy). Ist. di Ricerca sulle Acque; Spezia, S.; Bettinelli, M. [ENEL, Unified Modelling Language, Piacenza (Italy)

    1999-09-01

    An analytical procedure for the determination of total mercury in wastewaters and natural waters is described. Aqueous samples are fast digested with nitric acid by using the microwave-oven technique; the analysis of mercury is then performed by cold vapor atomic absorption spectrometry (CV-AAS) using two possible instrumental apparatus (batch system or flow injection). Sodium borohydride is used as the reducing agent for mercury in solution (Method A). The use of amalgamation traps on gold for the preconcentration of mercury lowers the detection limit of the analyte (Method B). [Italian] Viene descritta una procedura analitica per la determinazione del mercurio totale in acque di scarico e naturali. Il campione acquoso viene sottoposto a mineralizzazione con acido nitrico in forno a microonde e analizzato mediante spettroscopia di assorbimento atomico a vapori freddi (CV-AAS) in due possibili configurazioni strumentali (sistema batch oppure flow injection), utilizzando sodio boro idruro come agente riducente del mercurio (metodo A). L'impiego della trappola di oro per la preconcentrazione del mercurio mediante amalgama consente di determinare l'analita a livelli di pochi ng/L (metodo B).

  13. Efectos de los equipos de trabajo autogestionados (EQTA y del trabajo individual sobre características grupales e individuales

    Directory of Open Access Journals (Sweden)

    AITOR ARITZETA

    2001-01-01

    Full Text Available El presente trabajo observa la medida en la que la estructuración de los trabajadores, de una empresa de automoción, a partir de Equipos de Trabajo Autogestionados EQTA tiene efectos significativos con respecto a las 19 características grupales relacionadas con la efectividad(Campion, Medsker y Higgs, 1993. Partiendo de dos muestras estadísticamente independientes pertenecientes a la misma organización, se observa también, las diferencias que con respecto a la satisfacción laboral, compromiso grupal, autoestima y autonomía personal muestran los sujetos de los EQTA (N=188 y los que realizan su trabajo de forma individual (N= 44. Los resultados indican que los EQTA obtienen valores superiores, con respecto a los que trabajan individualmente, en 13 de las 19 características, estando 18 relacionadas con la satisfacción. Solamente 9 características se relacionaron con la satisfacción en los grupos de trabajo individual. El compromiso grupal, aunque estadísticamente igual en ambos grupos, aparece relacionado con la satisfacción en los EQTA. Los resultados obtenidos demuestran que los EQTA son beneficiosos, no solo económicamente, sino también para el desarrollo de los individuos en la organización. El trabajo tiene implicaciones para el diseño de los grupos autónomos en las organizaciones.

  14. Towards science educational spaces as dynamic and coauthored communities of practice

    Science.gov (United States)

    Dhingra, Koshi

    2008-04-01

    In this essay review, four studies around the themes of identity and globalization are summarized and analyzed. The researchers' perspectives are generally grounded in Brown and Campione's ideas on situated knowledge ( Classroom lessons: Integrating cognitive theory and classroom practice (pp. 229-270). Cambridge: The MIT Press/Bradford Books, 1994) and Lave and Wenger's definition of learning as an activity fostered through participation in communities of practice ( Situated learning. Legitimate peripheral participation. Cambridge: University of Cambridge Press, 1991). Questions about the goals of science education spaces, the nature of globalization in relation to practices in schools, the role of identities-in-practice in relation to participation in communities of practice such as classrooms are explored. Recommendations for key design features in effective science educational spaces, based upon the findings presented in the collection of four studies, are offered. School, it is suggested here, functions best as a clearing house for the myriad science-related stories student participants generate in their various communities of practice (e.g., within popular culture, family, community, informal educational sites). In this way, school has the potential to construct bridges between multiple student experiences and identities-in-practice.

  15. Die anderen Ausdrucksweisen: subtile Offensiven

    Directory of Open Access Journals (Sweden)

    Ella Jasiowka

    2002-03-01

    Full Text Available Was passiert, wenn der „Herrscher des filmischen Blickes“ eine Frau ist? Anhand zweier jeweils sehr unterschiedlicher Beispiele „weiblicher“ Filmkunst zeigt die Autorin, dass es nötig ist, herkömmliche Interpretationsmuster zu überwinden, um dem Filmschaffen einzelner Künstler und Künstlerinnen auf den Grund zu gehen. Da es nicht die „feministische Kunst“ an sich gibt, muß man für das Werk so unterschiedlicher Künstlerinnen wie Sally Potter und Jane Campion jeweils unterschiedliche Schlüssel suchen, um ihnen gerecht zu werden. Dies tut Radkiewicz in ihrem Werk – aus feministischer Perspektive. Sie holt jeweils verschieden weit aus, wenn es darum geht, künstlerische Herkunft, Biographie, Vorbilder und Ziele der Künstlerinnen zu deren Werk in Bezug zu setzten. Ohne zu bahnbrechend neuen Erkenntnissen zu kommen, gelingt es ihr doch, ein komplexes Bild der von ihr behandelten Filmemacherinnen und den Filmen zu entwerfen, das einen umfassenden Gesamteindruck vermittelt. Die Kürze des Buches bringt dabei eine Konzentration mit sich, die zu einer Weiterbeschäftigung und Vertiefung einlädt.

  16. Remedial actions for contaminated soil; Software di simulazione della bonifica di terreni contaminati

    Energy Technology Data Exchange (ETDEWEB)

    De Marco, S.; Bevilaqua, P. [Trieste Univ., Trieste (Italy). Dipt. di Ingegneria Chimica dell' Ambiente e delle Materie Prime

    2002-02-01

    Nowadays remediation of contaminated soils is possible using suitable technologies. The software herewith presented is a decisional support system that enables to verify the quality of soil after remediation. It only needs the results of laboratory analyses carried out on representative sample of contaminated soil. The software gives for a contaminated soil several remediation technologies; for each one it provides treatment costs, amount and quality of reclaimed soil. The software is done with dialog boxes that make it an easy tool also for non-skilled users. [Italian] Oggigiorno la bonifica dei terreni contaminati e' possibile mediante l'utilizzo di tecnologie appropriate. L'uso di un sistema decisionale, qual' e' il software di seguito presentato, permette di verificare, partendo da alcune analisi di laboratorio eseguite su un campione rappresentativo, la qualita' del terreno ottenuto dopo opportuna bonifica. Il sistema fornisce per un dato tipo di terreno inquinato diverse alternative impiantistiche e per ciascuna di esse presenta i costi di trattamento e la qualita' e i volumi di terreno bonificati. Il software e' realizzato mediante finestre di dialogo che lo rendono di facile utilizzo anche per non esperti del settore.

  17. Rare case of isolated osteochondroma of the zygomatic bone: an endoscopic-assisted approach.

    Science.gov (United States)

    Romano, Antonio; Dell'Aversana, Giovanni; Corvino, Raffaele; Abbate, Vincenzo; Iaconetta, Giorgio; Califano, Luigi

    2015-11-03

    L’osteocondroma è una neoplasia benigna che nel distretto cranio-facciale colpisce spesso il corpo ed il ramo mandibolare e soltanto di rado può interessare il processo coronoide e l’arco zygomatico. In questo articolo descriviamo un raro caso di osteocondroma isolato dell’osso zygomatico trattato mediante approccio chirurgico intraorale endoscopicamente assistito ed in letteratura non sono riportati altri casi di questa patologia trattati con procedure endoscopicamente assistite. Una donna caucasica di cinquantadue anni, è stata osservata nel nostro reparto nel mese di marzo del 2012, la paziente lamentava dolore in regione zygomatica destra. La tomografia computerizzata (TC) del distretto osseo interessato mostrava una lesione sessile non definita. Il sospetto diagnostico è stato di osteocondroma. L’intervento è stato realizzato in anestesia generale mediante approccio intraorale endoscopicamente assistito. All’esame istologico del campione operatorio è stata confermata la diagnosi di osteocondroma. Nell’immediato post operatorio la paziente non ha presentato edema, dolore o febbre. L’uso dell’endoscopia nel trattamento chirurgico di questa patologia quindi ha consentito di ottenere una maggiore precisione e una maggiore rispetto delle strutture anatomiche. L’approccio intraorale ci garantisce l’assenza di cicatrici visibili e l’ausilio dell’endoscopia ci consente di avere una migliore visione di tutte le strutture anatomiche, una buona gestione della patologia riducendo quindi il rischio di complicanze intraoperatorie quali: fratture patologiche e lesioni del VII nervo cranico.

  18. Personal Networks and Migration Decision: The Case of Jamaican Brain Drain

    Directory of Open Access Journals (Sweden)

    Rosalyn NEGRÓN

    2012-05-01

    Full Text Available Brain drain is the phenomena where the most educated citizens of a country migrate to countries with better opportunities. This typically affects developing countries more negatively than developed countries. Given the close proximity to the US and the high standard of education of its citizens, Jamaica tends to be particularly hard hit by this brain drain. In this paper I examine intentions to migrate among skilled and educated Jamaicans. Specifically, I explore to what extent the composition of their personal network affects their decision to migrate. The data set consists of 62 university students, roughly half of who intended to migrate. Data were collected on 40 people that they knew, including information about social support provided by their social networks. The socioeconomic data about respondents did not predict intentions to migrate. However, students at Campion College, a prestigious high school linked to upper middle class status, were significantly more likely to express an interest in migration than students from other schools. Frequency of travel abroad was negatively related to intention to migrate for those that had traveled at least once. The proportion of network members that provided informational and career advice was significantly higher for those that intended to migrate. Implications of these findings for immigration policy in Jamaica and receiving countries are discussed.

  19. Oscillazioni del mesone D^0

    CERN Document Server

    Schiraldi, Vito; Morello, Michael Joseph

    In fisica delle particelle, con il termine di "oscillazione" si indica la trasformazione di una particella neutra nella sua antiparticella e viceversa, fenomeno dovuto al fatto che gli autostati di flavor non sono autostati della matrice di massa. Il Modello Standard delle interazioni fondamentali predice che l'oscillazione del mesone D^0 avvenga su tempi molto maggiori di una vita media, e appaia perciò come un effetto piccolo, inferiore al percento, in contrasto con il caso dei mesoni B^0 e B^0_s che oscillano con molta maggiore rapidità. Sperimentalmente, l'oscillazione dei mesoni D^0 è rimasta inosservata fino a tempi recenti: la prima evidenza sperimentale dell'esistenza di un effetto di oscillazione è stata ottenuta soltanto nella primavera del 2007 dagli esperimenti BaBar (SLAC, USA) e Belle (KEKB, Japan). Nell'agosto 2007 anche l’esperimento CDF (Fermilab, USA) ha presentato una evidenza simile, basata sull'analisi di una parte del campione di dati disponibile. La tesi descrive gli aspetti speri...

  20. TYPHA × GLAUCA GODR., A HYBRID OF TYPHA L.(TYPHACEAE) NEWLY RECORDED IN CHINA AND ITS MORPHOLOGICAL CHARACTERISTICS%Typha × glauca Godr., 香蒲属(香蒲科)中国新记录杂种及其形态特征

    Institute of Scientific and Technical Information of China (English)

    朱秀玉; 王东

    2013-01-01

    Typha L. (Typhaceae), one of the most common aquatic plants found in marshes and shallow water, is almost cosmopolitan. However, the distribution of approximately 24 species has two main centers: Eurasia and North America. To date 12 species have been reported in China. Typha was described by Linnaeus in 1753, and its taxonomy remains confusing because of the variability in its reproductive and vegetative characteristics, and its frequent hybridization. Seven hybrids of Typha species were reported from North America and Europe but none of them has been recorded in China. We have discovered a hybrid cattail, Typha x glauca Godr. (T angustifolia L. x T latifolia L.), through extensive field collections and herbarium studies. That was a newly recorded taxon for China. We studied the hybrid T x glauca in the field and examined Typha collections from the following herbaria including PE, IBSC, CDBI, HIB, WU, SZ, and CCNU. Both vegetative and reproductive characteristics were measured for available specimens of the hybrid and parental species from China. Pollen grains of Typha species were collected from our field collections, and were examined under a light microscope at x 1000 magnification. Morphological features of the hybrid and parental species was illustrated and discussed, and an identification key for them was provided. The hybrid was morphologically variable and generally intermediate to the parental species. The most readily diagnostic features of the hybrid were: 1. leaves were glaucous; 2. pollen was typically a mixture of monads, dyads, triads, and tetrads (vs. tetrads in T latifolia and monads in T angustifolia), often abortive; 3. pistillate bracteoles were narrower than the stigma (vs. broader in T angustifolia and absent in T latifolia), and 4. the stigmas were liner-lanceolate (vs. linear in T angustifolia, and lanceolate to ovate-lanceolate in T. latifolia). Importantly, the presence /or absence of dyads and triads of pollen grains could be used to

  1. A whole plant approach to evaluate the water use of mediterranean maquis species in a coastal dune ecosystem

    Directory of Open Access Journals (Sweden)

    S. Mereu

    2009-02-01

    Full Text Available An integrated approach has been used to analyse the water relations of three Mediterranean species, A. unedo L., Q. ilex L. and P. latifolia L. co-occurring in a coastal dune ecosystem. The approach considered leaf level gas exchange, sap flow measurements and structural adaptations between 15 May and 31 July 2007, and was necessary to capture the different response of the three species to the same environment. The complexity of the response was proportional to the complexity of the system, characterized by a sandy soil with a low water retention capacity and the presence of a water table. The latter did not completely prevent the development of a drought response, and species differences in this responses have been partially attributed to a different root distribution. Sap flow of A. unedo decreased rapidly in response to the decline of Soil Water Content, while that of Q. ilex decreased only moderately. Midday leaf water potential of P. latifolia and A. unedo was between 2.2 and 2.7 MPa through the measuring period, while in Q. ilex it reached a value of 3.4 MPa at the end of the season. A. unedo was the only species to decrease the leaf area to sapwood area ratio from 23.9±1.2 (May to 15.2±1.5 (July, as a response to drought. A. unedo also underwent an almost stepwise loss on hydraulic conductivity, such a loss didn't occur for Q. ilex, while P. latifolia was able to slightly increase hydraulic conductivity, showing how different plant compartments coordinate differently between species as a response to drought. Such different coordination affects the gas exchange between vegetation and the atmosphere, and has implications for the response of the Mediterranean coastal dune ecosystems to climate change.

  2. Water level, vegetation composition, and plant productivity explain greenhouse gas fluxes in temperate cutover fens after inundation

    Science.gov (United States)

    Minke, Merten; Augustin, Jürgen; Burlo, Andrei; Yarmashuk, Tatsiana; Chuvashova, Hanna; Thiele, Annett; Freibauer, Annette; Tikhonov, Vitalij; Hoffmann, Mathias

    2016-07-01

    Peat extraction leaves a land surface with a strong relief of deep cutover areas and higher ridges. Rewetting inundates the deep parts, while less deeply extracted zones remain at or above the water level. In temperate fens the flooded areas are colonized by helophytes such as Eriophorum angustifolium, Carex spp., Typha latifolia or Phragmites australis dependent on water depth. Reeds of Typha and Phragmites are reported as large sources of methane, but data on net CO2 uptake are contradictory for Typha and rare for Phragmites. Here, we analyze the effect of vegetation, water level and nutrient conditions on greenhouse gas (GHG) emissions for representative vegetation types along water level gradients at two rewetted cutover fens (mesotrophic and eutrophic) in Belarus. Greenhouse gas emissions were measured campaign-wise with manual chambers every 2 to 4 weeks for 2 years and interpolated by modelling. All sites had negligible nitrous oxide exchange rates. Most sites were carbon sinks and small GHG sources. Methane emissions generally increased with net ecosystem CO2 uptake. Mesotrophic small sedge reeds with water table around the land surface were small GHG sources in the range of 2.3 to 4.2 t CO2 eq. ha-1 yr-1. Eutrophic tall sedge - Typha latifolia reeds on newly formed floating mats were substantial net GHG emitters in the range of 25.1 to 39.1 t CO2 eq. ha-1 yr. They represent transient vegetation stages. Phragmites reeds ranged between -1.7 to 4.2 t CO2 eq. ha-1 yr-1 with an overall mean GHG emission of 1.3 t CO2 eq. ha-1 yr-1. The annual CO2 balance was best explained by vegetation biomass, which includes the role of vegetation composition and species. Methane emissions were obviously driven by biological activity of vegetation and soil organisms. Shallow flooding of cutover temperate fens is a suitable measure to arrive at low GHG emissions. Phragmites australis establishment should be promoted in deeper flooded areas and will lead to moderate, but

  3. An integrated approach shows different use of water resources from Mediterranean maquis species in a coastal dune ecosystem

    Directory of Open Access Journals (Sweden)

    F. Manes

    2009-11-01

    Full Text Available An integrated approach has been used to analyse the dependence of three Mediterranean species, A. unedo L., Q. ilex L., and P. latifolia L. co-occurring in a coastal dune ecosystem on two different water resources: groundwater and rainfed upper soil layers. The approach included leaf level gas exchanges, sap flow measurements and structural adaptations between 15 May and 31 July 2007. During this period it was possible to capture different species-specific response patterns to an environment characterized by a sandy soil, with a low water retention capacity, and the presence of a water table. The latter did not completely prevent the development of a drought response and, combined with previous studies in the same area, response differences between species have been partially attributed to different root distributions. Sap flow of A. unedo decreased rapidly with the decline of soil water content, while that of Q. ilex decreased only moderately. Midday leaf water potential of P. latifolia and A. unedo ranged between −2.2 and −2.7 MPa throughout the measuring period, while in Q. ilex it decreased down to −3.4 MPa at the end of the season. A. unedo was the only species that responded to drought with a decrease of its leaf area to sapwood area ratio from 23.9±1.2 (May to 15.2±1.5 (July. While A. unedo also underwent an almost stepwise loss on hydraulic conductivity, such a loss did not occur for Q. ilex, whereas P. latifolia was able to slightly increase its hydraulic conducitivity. These differences show how different plant compartments coordinate differently between species in their responses to drought. The different responses appear to be mediated by different root distributions of the species and their relative resistances to drought are likely to depend on the duration of the periods in which water remains extractable in the upper soil layers.

  4. Biological Activity and Field Efficacy of Sulfosulfuron in Glasshouse and Wheat Field%磺酰磺隆的室内除草活性及对小麦田杂草田间防除效果

    Institute of Scientific and Technical Information of China (English)

    高兴祥; 高宗军; 房锋; 张悦丽; 齐军山; 李美

    2013-01-01

    [Aims] Sulfosulfuron is a new registered herbicide in our country. The paper aims to determine the biological activity and weed control effect of sulfosulfuron. [Methods] The whole plant dose-response experiments were conducted to evaluate the efficacy of sulfosulfuron on 11 major weeds growing in pots in glasshouse. Sulfosulfuron was applied postemergence to plots arranged in a randomized complete block design with four replications in wheat fields. [Results] The result of experiments in glasshouse showed that the sulfosulfuron had best effect on Capsella bursapastoris, Beckmannia syzigachne, Sclerochloa kengiana with the ED,, of 1.49, 0.98, 5.33 g a.i./ha respectively, displayed better effect on Galium spurium, Lithospermum arvense, Silene conoidea, and had good inhibition on the growth of Lolium multifloTum, Avena fatua, Bromus japonicus. It only had slightly control effect or no effect to Descuminia sophia and Aegilops tauschii. The results of field experiments also showed that sulfosulfuron had better control on Lamium amplexicaule, Galium aparina, Silene conoidea, Lithospermum arvense without causing injury to wheat. [Conclusions] It was illustrated that sulfosulfuron could be applied in wheat fields to control most broadleaf and grass weeds.%[目的]探讨磺酰磺隆对小麦田杂草的除草活性.[方法]采用室内生物测定方法测定了磺酰磺隆对11种麦田杂草的除草活性,并在小麦田进行了田间试验.[结果]温室试验表明:磺酰磺隆对荠菜、菵草和硬草有很好的防除效果,ED50值分别为1.49、0.98、5.33 g a.i./hm2;对猪殃殃、麦家公、麦瓶草也有较好的效果;对野燕麦等有较好的抑制生长作用,但对播娘蒿、节节麦效果较差.田间试验表明:磺酰磺隆对猪殃殃杂草等均有较好的防效且对小麦安全.[结论]磺酰磺隆可以应用于小麦田防除多种杂草.

  5. Insecticidal and Enzyme Inhibitory Activities of Sparassol and Its Analogues against Drosophila suzukii.

    Science.gov (United States)

    Kim, Junheon; Jang, Miyeon; Lee, Kyoung-Tae; Yoon, Kyungjae Andrew; Park, Chung Gyoo

    2016-07-13

    Drosophila suzukii is an economically important pest in America and Europe as well as in Asia. Sparassol and methyl orsellinate are naturally produced by the cultivating mushrooms Sparassis cripta and Sparassis latifolia. Fumigant and contact toxicities of synthetic sparassol and its analogues, methyl orsellinate and methyl 2,4-dimethoxy-6-methylbenzoate (DMB), were investigated. Negligible fumigant activity was observed from the tested compounds. However, DMB showed the strongest contact toxicity, followed by sparassol and methyl orsellinate. The possible modes of action of the compounds were assessed for their acetylcholinesterase (AChE)- and glutathione S-transferase (GST)-inhibiting activities. AChE activity was weakly inhibited by methyl orsellinate and DMB, but GST was inhibited by sparassol, methyl orsellinate, and DMB. Thus, DMB could be a promising alternative to common insecticides as it can be easily synthesized from sparassol, which is the natural product of Sparassis species. Sparassis species could be an industrial resource of DMB. PMID:27327201

  6. The effects of disturbance on forest structure and diversity at different altitudes in Garhwal Himalaya

    Institute of Scientific and Technical Information of China (English)

    Munesh KUMA; Chandra Mohan SHARMA; Govind Singh RAJWAR

    2009-01-01

    The effects of disturbance on forest structure and diversity along an altitudinal gradient in the temperate, sub-tropical, and tropical regions of Garhwal Himalaya were assessed. Each region was further categorized into undisturbed (UD), mildly disturbed (MD), and highly disturbed (HD) sites on the basis of magnitude of disturbance in these forests. On UD sites of temperate, sub-tropical and tropical regions, Quercus leucotrichophora, Anogeissus latifolia and Holoptelea integrifolia were the dominant tree species respectively. The highest values of tree density (1028 ind*hm-2) and total basal cover at breast height (31.70 m2 *hm-2) were recorded for UD site of temperate region, whereas maximum species diversity (3.128) and equitability (14.09) values were observed for HD site of tropical region. The structure and composition of the forests were greatly affected by the degree of disturbance.

  7. CRECIMIENTO Y PRODUCTIVIDAD DE CHAYA (Cnidoscolus chayamansa MCVAUGH, EUPHORBIACEAE) CON DENSIDAD DE PLANTACIÓN VARIABLE

    OpenAIRE

    Jesús Mao E. Aguilar Luna; Pedro A. Macario Mendoza; Esperanza Huerta Lwanga; Salvador Hernández Daumás; Ramiro de Alba Becerra; Eduardo García Villanueva

    2011-01-01

    Se evaluó el efecto que tiene la competenciainterespecífica en el crecimiento y la productividad de la chayacon densidad de plantación (DP) variable, en un suelocalcimórfico de tipo rendzina negra en Noh-Bec, Quintana Roo,México. La chaya se estableció a 1.50 x 3.00 m, utilizando estacassin hojas; se asoció con árboles jóvenes (menores de dosaños) de Cedrela odorata y Citrus latifolia en un diseño deplantación en círculo 'Nelder' de 3154 m2. Se establecieron ochoDP de 2602 a 3772 plantas.ha-1...

  8. Long-term ecological behaviour of abandoned uranium mill tailings

    International Nuclear Information System (INIS)

    Semi-aquatic and terrestrial areas on abandoned or inactive uranium mill tailings in Ontario were studied in order to identify the growth characteristics of the naturally invading species dominating these areas. Semi-aquatic areas of tailings sites have been invaded by cattails. These species formed wetland communities which varied in size, but all were essentially monocultures of Typha latifolia, T. angustifolia, or of the hybrids T. glauca. Sedges, Scripus cyperinus (wool-grass) and Phragmites australis (reed-grass), were found in transition zones between the cattail stand and the dry section of the tailings site. The expansion of the cattail stands appeared to be controlled by the hydrological conditions on the site, rather than the chemical characteristics of the tailings

  9. Curvas dose-resposta em espécies de plantas daninhas com o uso do herbicida glyphosate(1 Dose-response curves in weed species with the use of herbicide glyphosate

    Directory of Open Access Journals (Sweden)

    André Luiz de Souza Lacerda

    2004-01-01

    Full Text Available Com o objetivo de verificar a resposta de diferentes espécies de plantas daninhas ao herbicida glyphosate, foi desenvolvido em câmara de crescimento um experimento inteiramente casualizado, com cinco repetições no Departamento de Produção Vegetal da ESALQ/USP. Para isso, foram estudadas seis espécies de plantas daninhas: Bidens pilosa L., Tridax procumbens L., Digitaria insularis (L. Fedde, Spermacoce latifolia Aubl., Ipomoea grandifolia L. e Commelina benghalensis L. Utilizou-se o herbicida glyphosate, inibidor da enzima EPSPs, com doses em escalas de 0,0; 11,3; 22,5; 45; 90; 180; 360; 720 e 1.440 g.ha-1 i.a. A elaboração das curvas dose-resposta foi feita pelo ajuste da equação dos dados coletados de biomassa verde utilizando o modelo log-logístico: Y = C+D-C / 1 + Exp{b[log(x-log(RC50]}, calculado por modelo estatístico não linear dos dados pelo programa estatístico SAS. Após a determinação das curvas dose-resposta nas espécies acima citadas concluiu-se que Bidens pilosa foi considerada a planta daninha mais suscetível ao herbicida glyphosate, pois apresentou menor RC50 (31,86 g.ha-1 i.a.. As espécies Tridax procumbens, Digitaria insularis, Spermacoce latifolia, Ipomoea grandifolia, Commelina benghalensis obtiveram RC50 de 58,40; 128,50; 250,44; 615,49 e >1.440 g.ha-1 i.a. respectivamente. Portanto, a quantidade necessária de herbicida para reduzir em 50% a biomassa verde foi de 1,83; 4,03; 7,86; 32 e >359,56 vezes maior em relação à Bidens pilosa respectivamente.With the objective of veryfing the response of different doses in species of weeds by herbicide glyphosate, an experiment was conducted in growth chamber in a completetly randomized design with three repetitions, in the Department of Vegetable Production at ESALQ/USP. For that, were chosen six species of weeds: Bidens pilosa L., Tridax procumbens L., Digitaria insularis (L. Feed, Spermacoce latifolia Aubl., Ipomoea grandifolia L. and Commelina benghalensis L

  10. Utilization of emergent aquatic plants for biomass-energy-systems development

    Energy Technology Data Exchange (ETDEWEB)

    Kresovich, S.; Wagner, C.K.; Scantland, D.A.; Groet, S.S.; Lawhon, W.T.

    1982-02-01

    A review was conducted of the available literature pertaining to the following aspects of emergent aquatic biomass: identification of prospective emergent plant species for management; evaluation of prospects for genetic manipulation; evaluation of biological and environmental tolerances; examination of current production technologies; determination of availability of seeds and/or other propagules, and projections for probable end-uses and products. Species identified as potential candidates for production in biomass systems include Arundo donax, Cyperus papyrus, Phragmites communis, Saccharum spontaneum, Spartina alterniflora, and Typha latifolia. If these species are to be viable candidates in biomass systems, a number of research areas must be further investigated. Points such as development of baseline yield data for managed systems, harvesting conceptualization, genetic (crop) improvement, and identification of secondary plant products require refinement. However, the potential pay-off for developing emergent aquatic systems will be significant if development is successful.

  11. EFFECTIVENESS OF GLYPHOSATE AND 2.4 D AMIN HERBICIDES TO CONTROL WEEDS UNDER Shorea selanica Bl. PLANTATION IN CARITA TRIAL GARDEN, BANTEN

    Directory of Open Access Journals (Sweden)

    Ari Wibowo

    2007-03-01

    Full Text Available A trial was carried out in Carita, West Java, to identify the effectiveness of Glyphosate and 2.4 D Amin Herbicide to control weeds under Shorea selanica Bl. plantation. The trial was conducted through the application of Glyphosate and 2.4 D Amin Herbicide with dosages of 5, 6, 7, 8 and 9 liter per ha and compared with Glyphosate herbicide 5 liter per ha, manual treatment, and control (no treatment. The result showed that Glyphosate and 2.4 D Amin Herbicide could be used to control weeds in order to maintaining S. selanica Bl. plantation. Minimum dosage of 6 liter/ha was effective to control weeds such as Chromolaena odorata DC, Mikania micrantha Will, Lantana camara L, Imperata cylindrica Beauv., Melastoma malabathricum L, and Boreria latifolia Bl. Furthermore, there was no symptom of poison on S. selanica Bl. plantation after herbicide application with all dosages applied.

  12. Susceptibility of two-week old Lymnaea natalensis to some plant extracts.

    Science.gov (United States)

    Kela, S L; Ogunsusi, R A; Ogbogu, V C; Nwude, N

    1989-01-01

    The molluscacidal potency of 17 Nigerian plants extracted by the unevaporated crude water (UECW) method was evaluated on two-week old Lymnaea natalensis Krauss. Five extracts were not active but extracts of Balanites aegytiaca, Blighia sapida, Boswellia dalzielii, Cissampelos mucronata, Detarium microcarpum, Kigelia africana, Opilia celtidifolia, Parkia clappertoniana, Polygonum limbatum, Pseudocedrela kotschyi, Nauclea latifolia and Securidaca longipedunculata were molluscacidal. There is potential for their future use in the integrated control of Lymnaea natalensis, as well as other snails. Mortality data for lethal concentration values for all extracts were analysed by use of probit transformation. The upper and lower fiducial limits of the LC50 (P = 0.05) were also determined. PMID:2626571

  13. Screening of some Nigerian plants for molluscicidal activity.

    Science.gov (United States)

    Kela, S L; Ogunsusi, R A; Ogbogu, V C; Nwude, N

    1989-01-01

    Methanolic (MEOH), evaporated crude water (ECW) and unevaporated crude water (UECW) extracts of 25 Nigerian plants, used for different medicinal and domestic purposes were screened for molluscacidal activity on laboratory-reared Lymnaea natalensis Krauss. Seven of the plants were not active; extracts from 18 (72 per cent) of the plants, some of which are renowned fish poisons, had molluscicidal activity. These were Acacia nilotica, Aristolochia albida, Balanites aegyptiaca, Blighia sapida, Boswellia dalzielii, Detarium microcarpum, Gnidia kraussiana, Kigelia africana, Nauclea latifolia, Opilia celtidefolia, Parkia clappertoniana, Polygonum limbatum, Pseudocedrela kotschyi, Sclerocarya birrea, Securidaca longipedunculata, Ximenia americana, Vetiveria nigritana and Ziziphus abyssinica. The LC50 of these extracts were determined. It is strongly recommended that the toxic effects of these extracts against fish, cercariae, snail eggs and mammals be further investigated so as to determine the right concentration, especially for use in fish ponds. PMID:2626572

  14. Constructed wetland as an ecotechnological tool for pollution treatment for conservation of Ganga river.

    Science.gov (United States)

    Rai, U N; Tripathi, R D; Singh, N K; Upadhyay, A K; Dwivedi, S; Shukla, M K; Mallick, S; Singh, S N; Nautiyal, C S

    2013-11-01

    With aim to develop an efficient and ecofriendly approach for on-site treatment of sewage, a sub-surface flow constructed wetland (CW) has been developed by raising potential aquatic macrophytes; Typha latifolia, Phragmites australis, Colocasia esculenta, Polygonum hydropiper, Alternanthera sessilis and Pistia stratoites in gravel as medium. Sewage treatment potential of CW was evaluated by varying retention time at three different stages of plant growth and stabilization. After 6 months, monitoring of fully established CW indicated reduction of 90%, 65%, 78%, 84%, 76% and 86% of BOD, TSS, TDS, NO3-N, PO4-P and NH4-N, respectively in comparison to inlet after 36 h of retention time. Sewage treatment through CW also resulted in reduction of heavy metal contents. Thus, CW proved an effective method for treatment of wastewater and may be developed along river Ganga stretch as an alternative technology. Treated water may be drained into river to check further deterioration of Ganga water quality.

  15. Development of a Competent and Trouble Free DNA Isolation Protocol for Downstream Genetic Analyses in Glycine Species

    Directory of Open Access Journals (Sweden)

    Muhammad Amjad Nawaz

    2016-08-01

    Full Text Available Extraction of deoxyribose nucleic acid (DNA from plants is preliminary step in molecular biology. Fast and cost effective genomic DNA isolation from Glycine species for downstream application is a major bottleneck. Here we report a high throughput and trouble free method for genomic DNA extraction from leaf and seeds of Glycine species with high quality and quantity. Protocol reports the optimization by employing different concentrations of CTAB and PVP in extraction buffer. Efficiency of optimized protocol was compared with frequently used DNA extraction methods. Wide adoptability and utility of this protocol was confirmed by DNA extraction from leaves as well as seeds of G. max, G. soja, G. tomentella and G. latifolia. Extracted DNA was successfully subjected to PCR amplification of five microsatellite markers and four putative glycosyltransferase genes. DNA extraction protocol is reproducible, trouble free, rapid and can be adopted for plant molecular biology applications.

  16. Exploration of High elevation liana colonies on Mt. Slamet, Central Java, Indonesia

    Directory of Open Access Journals (Sweden)

    WS Hoover

    2009-12-01

    Full Text Available One hundred forty–five individual lianas were distributed on 2 East facing ridges on the second highest mountain on Java, Mt. Slamet (3418 m., Central Java, Indonesia. Twenty one colonies were observed on small flat areas on ridges. The liana species observed include: Embelia pergamacea, Toddalia asiatica, Elaeagnus latifolia, Schefflera lucida, Vaccinium laurifolium and Lonicera javanica. Diameter of each liana was measured and liana density/flat area calculated. Floristic collecting was under- taken within the elevational gradient of liana distribution. Data suggest an ecotone transition from lower to upper mon- tane forest is observed between 2200 and 2300 m, though forest types are difficult to determine due to disturbance caused by fire at the upper elevations. Observing lianas at these unusuall high elevations with near pluvial rainfall, con- tradict established scientific theory concerning global distribution and abundance of lianas.  

  17. The first record of Baccharis L. (Asteraceae) as a host-plant genus for Nepticulidae (Lepidoptera), with description of new Stigmella species from South America.

    Science.gov (United States)

    Stonis, Jonas R; Diškus, Arūnas; Remeikis, Andrius; Remeikis, Andrius; Davis, Donald R; Solis, M Alma; Torres, Nixon Cumbicus

    2016-01-01

    We record the first Nepticulidae species found to feed on Baccharis L. (Asteraceae). Despite the high species richness of Baccharis in the Western Hemisphere, no nepticulid has ever been recorded feeding on Baccharis. In this paper we describe six new Stigmella Schrank species feeding on Baccharis: S. emarginatae Diškus & Stonis, sp. nov., S. bipartita Diškus & Stonis, sp. nov., S. tripartita Diškus & Stonis, sp. nov., S. latifoliae Remeikis, Diškus & Stonis, sp. nov., S. baccharicola Diškus & Stonis, sp. nov., and S. confertae Diškus & Stonis, sp. nov. The remaining two taxa are left unnamed. All taxa are illustrated with photographs of adults, their genitalia, and their leaf-mines. Additionally, leaf-mines on Baccharis salicifolia are documented. PMID:27395706

  18. Fatty acid composition of oil synthesized by Aspergillus nidulans.

    Science.gov (United States)

    Sharma, N D; Mathur, J M; Saxena, B S; Sen, K

    1981-01-01

    The filamentous fungus Aspergillus nidulans Eidam strain 300 was found to be capable of synthesizing 24.9% oil or remarkably low free fatty acidity, in a chemically defined medium with 34% glucose as sole carbon source. although the total content of oil synthesized was less, utilization of the carbon source is better as shown by the high (8.4) fat coefficient. The major component fatty acids of the oil were palmitic, stearic, oleic and linoleic and are influenced by the source of carbon. Palmitoleic acid is present in traces, confirming thereby the general observation that high oil formers produce oil of low hexadecenoic acid content. The relatively high stearic acid content of the oil distinguishes it from those of other microorganisms and resembles the oil produced by certain tropical plants, such as Madhuca latifolia. PMID:7026394

  19. Emission of isoprene from common Indian plant species and its implications for regional air quality.

    Science.gov (United States)

    Singh, Rashmi; Singh, Abhai Pratap; Singh, M P; Kumar, Animesh; Varshney, C K

    2008-09-01

    Isoprene is most dominant volatile organic compounds (VOC) emitted by many plants. In this study 40 common Indian plant species were examined for isoprene emission using dynamic flow through enclosure chamber technique. Isoprene emission rates of plants species were found to vary from undetectable to 69.5 microg g(-1) h(-1) (Madhuca latifolia). Besides, an attempt has been made to evaluate suitability of 80 common Indian plant species for planting programmes. Out of 80 species, 29 species were moderate to high emitters (10 to < or =25 microg g(-1) h(-1)), 12 species were low emitter emitters (1 to < or =10 microg g(-1) h(-1)) and remaining 39 species were found to be negligible or non emitters (<1 microg g(-1) h(-1)) of isoprene. About 50% plant species selected for planting programmes in India were found to be moderate to high emitters of isoprene. PMID:17952698

  20. Studies on production and biological potential of prodigiosin by Serratia marcescens.

    Science.gov (United States)

    Suryawanshi, Rahul K; Patil, Chandrashekhar D; Borase, Hemant P; Salunke, Bipinchandra K; Patil, Satish V

    2014-07-01

    Efficacy of Serratia marcescens for pigment production and biological activity was investigated. Natural substrates like sweet potato, mahua flower extract (Madhuca latifolia L.), and sesam at different concentrations were taken. As a carbon source microorganism favored potato powder was followed by sesam and mannitol, and as nitrogen source casein hydrolysate was followed by yeast and malt extract. The effect of inorganic salts on pigment production was also studied. At final optimized composition of suitable carbon, nitrogen source, and trace materials and at suitable physiological conditions, prodigiosin production was 4.8 g L(-1). The isolated pigment showed antimicrobial activity against different pathogenic bacteria and fungi. Extracted pigment was characterized by spectroscopy, Fourier transform infrared (FTIR), and thin layer chromatography (TLC) which confirm production of biological compound prodigiosin. This study suggests that use of sweet potato powder and casein can be a potential alternative bioresource for commercial production of pigment prodigiosin. PMID:24781979

  1. THE ALLIANCE TRACHYNION DISTACHYAE RIVAS-MARTINEZ 1978 IN CENTRAL ITALYIN CENTRAL ITALY

    Directory of Open Access Journals (Sweden)

    M. DE SANCTIS

    2010-01-01

    Full Text Available The therophytic basiphilous vegetation of Central Italy has been generally framed in a single association Trifolio scabri- Hypochoeridetum achyrophori Biondi et al. 1997. We show that a considerable floristic variation exists inside Trifolio-Hypochoeridetum in Central Italy, and that it should be treated as a complex of no less than five different associations: Medicagini rigidulae-Trifolietum scabri Fanelli et al. hoc loco, Trifolio scabri-Hypochoeridetum achyrophori Biondi, Izco. Ballelli & Formica 1997 s.s, Hippocrepido siliquosae- Brachypodietum distachyi Fanelli et al. ad interim, Trigonello gladiatae-Brachypodietum distachyi Fanelli et al. hoc loc, Crucianello latifoliae-Hypochoeridetum achyrophori Filesi, Blasi, Di Marzio 1996. These associations are floristically and ecologically distinct, and show different geographical ranges in Central Italy. The great diversity of Trachynion distachyae in Central Italy is related to the widespread occurrence of limestone and calcareous alluvial rocks and to a climate with abundant winter and autumn rains very favourable to the development of winter annuals.

  2. Virucidal activity of essential oils from aromatic plants of San Luis, Argentina.

    Science.gov (United States)

    García, C C; Talarico, L; Almeida, N; Colombres, S; Duschatzky, C; Damonte, E B

    2003-11-01

    Essential oils obtained from eight aromatic plants of San Luis Province, Argentina, were screened for virucidal activity against herpes simplex virus type 1 (HSV-1), Junin virus (JUNV) and dengue virus type 2 (DEN-2). The most potent inhibition was observed with the essential oil of Lippia junelliana and Lippia turbinata against JUNV with virucidal concentration 50% (VC(50)) values in the range 14-20 ppm, whereas Aloysia gratissima, Heterotheca latifolia and Tessaria absinthioides inhibited JUNV in the range 52-90 ppm. The virucidal activity was time- and temperature-dependent. The essential oils of A. gratissima, Artemisia douglasiana, Eupatorium patens and T. absinthioides inactivated HSV-1 at 65-125 ppm. However, only A. douglasiana and E. patens had any discernible effect on DEN-2 infectivity with VC(50) values of 60 and 150 ppm, respectively. PMID:14595590

  3. Constructed wetland as an ecotechnological tool for pollution treatment for conservation of Ganga river.

    Science.gov (United States)

    Rai, U N; Tripathi, R D; Singh, N K; Upadhyay, A K; Dwivedi, S; Shukla, M K; Mallick, S; Singh, S N; Nautiyal, C S

    2013-11-01

    With aim to develop an efficient and ecofriendly approach for on-site treatment of sewage, a sub-surface flow constructed wetland (CW) has been developed by raising potential aquatic macrophytes; Typha latifolia, Phragmites australis, Colocasia esculenta, Polygonum hydropiper, Alternanthera sessilis and Pistia stratoites in gravel as medium. Sewage treatment potential of CW was evaluated by varying retention time at three different stages of plant growth and stabilization. After 6 months, monitoring of fully established CW indicated reduction of 90%, 65%, 78%, 84%, 76% and 86% of BOD, TSS, TDS, NO3-N, PO4-P and NH4-N, respectively in comparison to inlet after 36 h of retention time. Sewage treatment through CW also resulted in reduction of heavy metal contents. Thus, CW proved an effective method for treatment of wastewater and may be developed along river Ganga stretch as an alternative technology. Treated water may be drained into river to check further deterioration of Ganga water quality. PMID:24080292

  4. A case of fasciolasis hepatic in Zunyi City,Guizhou Province%肝片形吸虫病1例

    Institute of Scientific and Technical Information of China (English)

    王锐峰; 唐玲静; 周爱明

    2014-01-01

    Four adults of Fasciola hepatica were found from the bile ducts of a patient diagnosed as biliary calculi during a surgi-cal operation. We investigated retrospectively the infection source and concluded that the patient may be infected by eating raw or half-cooked Zizania latifolia,an aquatic plant,which was contaminated with metacercariae of F. hepatica.%报道1例胆管结石嵌顿患者,术中发现4条肝片形吸虫。对患者进行了感染源追溯,该病例可能由于生食或半生食含有囊蚴的水生植物茭白而感染。

  5. The phytoremediation ability of a polyculture constructed wetland to treat boron from mine effluent

    Energy Technology Data Exchange (ETDEWEB)

    Türker, Onur Can [Faculty of Science and Letters, Department of Biology, Aksaray University, Aksaray (Turkey); Böcük, Harun, E-mail: hbocuk@anadolu.edu.tr [Faculty of Science, Department of Biology, Anadolu University, Eskişehir (Turkey); Yakar, Anıl [Faculty of Science, Department of Biology, Anadolu University, Eskişehir (Turkey)

    2013-05-15

    Highlights: ► We assessed the phytoremediation ability of a polyculture constructed wetland (PCW) to treat boron (B) from mine effluent. ► B in mine effluent decreased from 187 mg l{sup −1} to 123 mg l{sup −1} (32% removal rate) through the PCW. ► Estimated methane production, energy yields and electrical energy yields of the PCW increased with biomass production. ► Cattails accumulated more than 250 mg kg{sup −1} B and common reed accumulated 38 mg kg{sup −1} B at the end of the experiment. -- Abstract: This study focuses on describing the ability of a small-scale, subsurface-flow-polyculture-constructed wetland (PCW) to treat boron (B) mine effluent from the world's largest borax mine (Kırka, Turkey) under field conditions. This application is among the first effluent treatment methods of this type in both Turkey and the world. This study represents an important resource on how subsurface-flow-constructed wetlands could be used to treat B mine effluents in the field conditions. To this end, an experimental wetland was vegetated with common reed (Phragmites australis) and cattails (Typha latifolia), and mine effluent was moved through the wetland. The results of the present study show that B concentrations of the mine effluent decreased from 187 to 123 mg l{sup −1} (32% removal rate) on average. The T. latifolia individuals absorbed a total of 250 mg kg{sup −1} whereas P. australis in the PCW absorbed a total of 38 mg kg{sup −1} B during the research period.

  6. Comparative responses of freshwater organisms to exposures of a commercial naphthenic acid.

    Science.gov (United States)

    Kinley, Ciera M; McQueen, Andrew D; Rodgers, John H

    2016-06-01

    Comparative toxicity studies using unconfounded exposures can prioritize the selection of sensitive sentinel test species and refine methods for evaluating ecological risks of complex mixtures like naphthenic acids (NAs), a group of organic acids associated with crude oils and energy-derived waters that have been a source of aquatic toxicity. The objectives of this study were to compare responses of freshwater aquatic organisms (vertebrate, invertebrates, and a macrophyte; in terms of acute toxicity) to Fluka commercial NAs and to compare measured toxicity data with peer-reviewed toxicity data for other commercial NA sources and energy-derived NA sources. Exposures were confirmed using high performance liquid chromatography. Responses (7-d LC50s/EC50) ranged from 1.9 mg L(-1) for Pimephales promelas to 56.2 mg L(-1) for Typha latifolia. Following P. promelas in order of decreasing sensitivity were Ceriodaphnia dubia (7-d LC50 = 2.8 mg L(-1)), Hyalella azteca (7-d LC50 = 4.1 mg L(-1)), Chironomus dilutus (7-d LC50 = 6.5 mg L(-1)), and T. latifolia (7-d EC50 = 56.2 mg L(-1)), indicating that in terms of sensitivities, fish > invertebrates > plant for Fluka NAs in this study. Factors that affect exposures and measurements of exposures differ among commercial and energy-derived NAs and constrain comparisons. Despite differences in exposures, fish and invertebrates were relatively sensitive to both commercial and energy-derived NA sources (based on laboratory measurements and peer-reviewed data) and could be appropriate sentinel species for risk evaluations. PMID:27016812

  7. Self-medication with tannin-rich browse in goats infected with gastro-intestinal nematodes.

    Science.gov (United States)

    Amit, M; Cohen, I; Marcovics, A; Muklada, H; Glasser, T A; Ungar, E D; Landau, S Y

    2013-12-01

    Primates self-medicate to alleviate symptoms caused by gastro-intestinal nematodes (GIN) by consuming plants that contain secondary compounds. Would goats display the same dietary acumen? Circumstantial evidence suggests they could: goats in Mediterranean rangelands containing a shrub - Pistacia lentiscus - with known anthelmintic properties consume significant amounts of the shrub, particularly in the fall when the probability of being infected with GIN is greatest, even though its tannins impair protein metabolism and deter herbivory. In order to test rigorously the self-medication hypothesis in goats, we conducted a controlled study using 21 GIN-infected and 23 non-infected goats exposed to browse foliage from P. lentiscus, another browse species - Phillyrea latifolia, or hay during the build-up of infection. GIN-infected goats showed clear symptoms of infection, which was alleviated by P. lentiscus foliage but ingesting P. lentiscus had a detrimental effect on protein metabolism in the absence of disease. When given a choice between P. lentiscus and hay, infected goats of the Mamber breed showed higher preference for P. lentiscus than non-infected counterparts, in particular if they had been exposed to Phillyrea latifolia before. This was not found in Damascus goats. Damascus goats, which exhibit higher propensity to consume P. lentiscus may use it as a drug prophylactically, whereas Mamber goats, which are more reluctant to ingest it, select P. lentiscus foliage therapeutically. These results hint at subtle trade-offs between the roles of P. lentiscus as a food, a toxin and a medicine. This is the first evidence of self-medication in goats under controlled conditions. Endorsing the concept of self-medication could greatly modify the current paradigm of veterinary parasitology whereby man decides when and how to treat GIN-infected animals, and result in transferring this decision to the animals themselves. PMID:24140164

  8. Fertilizer comparison test of the special formula fertilizer for Zizania latifoia%茭白配方专用肥肥效比较试验

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

      通过与常规施肥比较,研究茭白配方专用肥对茭白生物学性状、产量和品质的影响。试验结果表明:茭白商品性有明显改善,粗脂肪、维生素C、总糖、氨基酸等品质指标增幅达到极显著水平,并可实现增产增效21.96%,节约肥料成本6.53%,利润提高25.98%。在配方专用肥的基础上增施氮肥,不能提高茭白产量,且肥料成本增加,种植效益明显下降。%In this study, by comparing the conventional fertilization with the special formula fertilizer for Zizania latifolia, the effect of the special formula fertilizer on biological characters, yield and quality were studied. The results show that there is a significant improvement in Zizania latifolia commodity traits, and the crude fat, vitamin C, total sugars, amino acids, and other quality indicators are increased significantly. Appling special formula fertilizer results in 21.96% increase in yield and efficiency, a 6.53% reduction in fertilizer costs, a 25.98% increase in profit. In addition, adding nitrogen fertilizer on the basis of the formula specialty fertilizer can’t improve Zizania latifolia’s production, and planting efficiency decreases with the increase of fertilizer costs.

  9. Phytochemical, Antimicrobial, and Toxicological Evaluation of Traditional Herbs Used to Treat Sore Throat

    Directory of Open Access Journals (Sweden)

    Arifa Mehreen

    2016-01-01

    Full Text Available The in vitro antibacterial activities of 29 traditional medicinal plants used in respiratory ailments were assessed on multidrug resistant Gram-positive and Gram-negative bacteria isolated from the sore throat patients and two reference strains. The methanolic, n-hexane, and aqueous extracts were screened by the agar well diffusion assay. Bioactive fractions of effective extracts were identified on TLC coupled with bioautography, while their toxicity was determined using haemolytic assay against human erythrocytes. Qualitative and quantitative phytochemical analysis of effective extracts was also performed. Methanolic extract of 18 plants showed antimicrobial activity against test strains. Adhatoda vasica (ZI = 17–21 mm, MIC: 7.12–62.5 μg/mL, Althaea officinalis (ZI = 16–20 mm, MIC: 15.62–31.25 μg/mL, Cordia latifolia (ZI = 16–20 mm, MIC: 12.62–62.5 μg/mL, Origanum vulgare (ZI = 20–22 mm, MIC: 3–15.62 μg/mL, Thymus vulgaris (ZI = 21–25 mm, MIC: 7.81–31.25 μg/mL, and Ziziphus jujuba (ZI = 14–20 mm, MIC: 7.81–31.25 μg/mL showed significant antibacterial activity. Alkaloid fractions of Adhatoda vasica, Cordia latifolia, and Origanum vulgare and flavonoid fraction of the Althaea officinalis, Origanum vulgare, Thymus Vulgaris, and Ziziphus jujuba exhibited antimicrobial activity. Effective plant extracts show 0.93–0.7% erythrocyte haemolysis. The results obtained from this study provide a scientific rationale for the traditional use of these herbs and laid the basis for future studies to explore novel antimicrobial compounds.

  10. Indicator species of essential forest tree species in the Burdur district.

    Science.gov (United States)

    Negiz, Mehmet Güvenç; Eser, Yunus; Kuzugüdenll, Emre; Izkan, Kürşad

    2015-01-01

    The forests of Burdur district for long have been subjected to over grazing and individual selection. As a result of this, majority of the forest areas in the district were degraded. In the district, afforestation efforts included majority of forestry implementations. It is well known that selecting suitable species plays an important role for achieving afforestation efforts. In this context, knowing the indicator species among the target species would be used in afforestation efforts, studies on the interrelationships between environmental factors and target species distribution is vital for selecting suitable species for a given area. In this study, Anatolian Black pine (Pinus nigra), Red pine (Pinus brutia), Crimean juniper (Juniperus excelsa) and Taurus cedar (Cedrus libani), essential tree species, were considered as target species. The data taken from 100 sample plots in Burdur district was used. Interspecific correlation analysis was performed to determine the positive and negative indicator species among each of the target species. As a result of ICA, 2 positive (Berberis crataegina, Juniperus oxycedrus), 2 negative (Phillyrea latifolia, Quercus coccifera) for Crimean Juniper, I positive (Juniperus oxycedrus), 3 negative (Onopordium acanthium, Fraxinus ornus, Phillyrea latifolia) for Anatolian black pine, 3 positive (Paliurus spina-christi, Quercus coccifer, Crataegus orientalis), 2 negative (Berberis crataegina, Astragalus nanus) for Red pine and 3 positive (Berberis crataegina, Rhamnus oleoides, Astragalus prusianus) 2 negative (Paliurus spina-christi, Quercus cerris) for Taurus cedarwere defined as indicator plant species. In this way, practical information was obtained for selecting the most suitable species, among the target species, for afforestation efforts in Burdur district. PMID:26591889

  11. Structure of aquatic vegetation of a large lake, western border of the Brazilian Pantanal.

    Science.gov (United States)

    Cunha, N L; Delatorre, M; Rodrigues, R B; Vidotto, C; Gonçalves, F; Scremin-Dias, E; Damasceno-Júnior, G; Pott, V J; Pott, A

    2012-08-01

    Studies on Neotropical aquatic macrophytes have increased in recent decades, however species richness in wetlands of South America is far from being fully known. In addition, studies having an ecological approach are scarce in the Pantanal. Rapid assessments are essential for gaining knowledge of the biodiversity in the region. This study was performed in five sites of the Baía do Castelo, the western border of the Brazilian Pantanal, which included wild-rice patches, floating mats and floating meadows. At each site, plots of 0.5 × 0.5 m were set (n = 137), species of aquatic macrophytes were identified, their coverage was measured and the plot depth was estimated. We recorded 57 species in 26 families, of which Poaceae was the richest. The most frequent and abundant species was Commelina schomburgkiana; the second most frequent was Oryza latifolia,followed by Leersia hexandra, Enydra radicans and Pityrogramma calomelanos. The latter species was second in cover, followed by Pontederia rotundifolia, Eichhornia azurea, E. crassipes and Enydra radicans. These five species and C. schomburgkiana (the most abundant) together represent more than half of the coverage on the lake. Pontederia rotundifolia, Ludwigia helminthorrhiza, Pistia stratiotes, E. azurea, E. crassipes, Enydra radicans and Panicum elephantipes were strongly associated with deeper areas, while Oryza latifolia, Leersia hexandra and Salvinia auriculata were prevalent in shallow areas. Pityrogramma calomelanos, Ludwigia nervosa, Ipomoea alba, Cayaponia podantha, Polygonum acuminatum, Rhynchanthera novemnervia and Ludwigia leptocarpa were highly correlated with floating meadows. The structure of the habitat, natural dynamics and zonation of aquatic vegetation in the Baía do Castelo seems to be influenced by a variation in water levels, which promotes spatial segregation, most likely due to competition and/habitat preference. PMID:22990823

  12. New Discoveries of Phaeophycean Fossils in the Early Cambrian,Haikou, Kunming,Yunnan, Southwest China%云南海口早寒武世褐藻化石新知

    Institute of Scientific and Technical Information of China (English)

    徐兆良

    2001-01-01

    主要报道了产自云南海口耳材村早寒武世筇竹寺组玉案山段澄江生物群中的褐藻化石——拟点叶藻(Punctariopsis latifolia gen. et sp. nov.)、简单拟点叶藻(Punctariopsis simplex gen. et sp. nov.)和古文德带藻(Vendotaenia cf. antiqua Gnilovskaya) 3种.这些化石丰富了澄江生物群物种的多样性,为研究该群生物爆发性演化提供了新的证据.同时通过对现生褐藻和褐藻化石形态学的比较研究,表明化石属和现代属在系统演化上可能有较密切的亲缘关系,并进一步证明了澄江生物群在云南海口地区发生于一个潮间带和亚潮带的海水环境.%The paper deals with the new phaeophycean fossils in the Early Cambrian from Chengjiang Biota at Ercai Village of Haikou, Kunming, Yunnan, Southwest China. Three new taxa, Punctariopsis latifolia gen. et sp. nov., P.simplex gen. et sp. nov. and Vendotaenia cf. antiqua Gnilovskaya are described here in detail. These new findings show that the Chengjiang Biota is high in species diversity of fossil algae and might offer new evidence for a better understanding of the Cambrian explosive biological evolution. The fossil genus described here is found to have a close relationship with the living Punctaria based on similar morphology. The results suggest that the Chengjiang Biota in Haikou area of Kunming was living in an intertidal and subtidal seawater environment.

  13. Eficiência do triclopyr no controle de plantas daninhas em gramado (Paspalum notatum Triclopyr efficiency in controlling weeds in the lawn (Paspalum notatum

    Directory of Open Access Journals (Sweden)

    F.C.L. Freitas

    2003-04-01

    Full Text Available As plantas daninhas interferem nos gramados, prejudicando a sua formação, qualidade e estética. O carrapicho-beiço-de-boi (Desmodium incanum e a zórnia (Zornia latifolia, quando não controlados, podem atingir níveis de infestação que chegam a dizimar o gramado. Dentre as diversas técnicas de manejo de plantas daninhas em gramados, destaca-se o emprego de herbicidas; todavia, as informações sobre a utilização desses produtos em gramados são escassas. O objetivo deste trabalho foi avaliar a eficiência do herbicida triclopyr no controle de Desmodium incanum e Zornia latifolia, infestantes da grama-batatais (Paspalum notatum. O trabalho foi desenvolvido em gramado estabelecido no campus da Universidade Federal de Viçosa, em Viçosa-MG, entre os meses de abril e maio de 2000. Os tratamentos avaliados foram seis doses do triclopyr (0,00; 0,24; 0,48; 0,72; 0,96; e 1,20 kg ha-1 e uma dose do 2,4-D + picloram (0,48 + 0,128 kg ha-1, dispostos em delineamento experimental com blocos ao acaso e quatro repetições. O tamanho de cada parcela foi de 12 m² (4,0 x 3,0 m, sendo os tratamentos aplicados em uma faixa de 2,0 m de largura, ficando 1,0 m como testemunha lateral, para auxiliar nas avaliações visuais. Foram realizadas avaliações de fitotoxicidade aos 10 e 20 DAT (Dias Após o Tratamento e nível de controle aos 15 e 30 DAT, ambos com base na testemunha lateral. Em nenhum dos tratamentos avaliados verificaram-se danos na qualidade do gramado. A partir das doses de 0,48 e 0,66 kg ha-1 de triclopyr foram obtidos controles iguais ou superiores a 90% de Desmodium incanum e Zornia latifolia, respectivamente. Esses resultados foram semelhantes ao do tratamento-padrão (2,4D + picloram a 0,48 + 0,128 kg ha-1. Concluiu-se que o triclopyr mostrou-se seletivo para grama-batatais, proporcionando excelente controle das plantas daninhas acima mencionadas.Weeds interfere in the formation, quality and aesthetics of the lawn. Desmodium incanum

  14. Naturally-assisted metal phytoextraction by Brassica carinata: Role ofroot exudates

    Energy Technology Data Exchange (ETDEWEB)

    Quartacci, Mike F., E-mail: mfquart@agr.unipi.i [Dipartimento di Chimica e Biotecnologie Agrarie, Universita di Pisa, Via del Borghetto 80, 56124 Pisa (Italy); Irtelli, Barbara [Dipartimento di Chimica e Biotecnologie Agrarie, Universita di Pisa, Via del Borghetto 80, 56124 Pisa (Italy); Gonnelli, Cristina; Gabbrielli, Roberto [Dipartimento di Biologia Vegetale, Sezione di Ecologia e Fisiologia Vegetale, Universita di Firenze, Via Micheli 1, 50121 Firenze (Italy); Navari-Izzo, Flavia [Dipartimento di Chimica e Biotecnologie Agrarie, Universita di Pisa, Via del Borghetto 80, 56124 Pisa (Italy)

    2009-10-15

    Due to relatively high chelant dosages and potential environmental risks it is necessary to explore different approaches in the remediation of metal-contaminated soils. The present study focussed on the removal of metals (As, Cd, Cu, Pb and Zn) from a multiple metal-contaminated soil by growing Brassica carinata plants in succession to spontaneous metallicolous populations of Pinus pinaster, Plantago lanceolata and Silene paradoxa. The results showed that the growth of the metallicolous populations increased the extractable metal levels in the soil, which resulted in a higher accumulation of metals in the above-ground parts of B. carinata. Root exudates of the three metallicolous species were analysed to elucidate their possible role in the enhanced metal availability. The presence of metals stimulated the exudation of organic and phenolic acids as well as flavonoids. It was suggested that root exudates played an important role in solubilising metals in soil and in favouring their uptake by roots. - Phytoextraction of metals is enhanced in Brassica carinata grown in succession to metallicolous populations of spontaneous species.

  15. The program of international intercomparison of accident dosimetry; Le programme d'intercomparaison internationale de dosimetrie d'accident 10-12 juin 2002

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-06-01

    The French institute of radioprotection and nuclear safety (IRSN) has carried out in June 2002 an international intercomparison program for the testing of the physical and biological accident dosimetry techniques. The intercomparison is jointly organized by the IRSN and the OECD-NEA with the sustain of the European commission and the collaboration of the CEA centre of Valduc (France). About 30 countries have participated to this program. Each country has supplied its own dosimeters and biological samples which have been irradiated using the Silene reactor of CEA-Valduc or a {sup 60}Co source. These experiments allow to test the new dosimetric techniques that have been developed since the previous intercomparison program (1993) and to confirm or improve the performances of older techniques. Aside from the intercomparison exercise, this report makes a status of the known radiological accidents and of the effects of high doses of ionizing radiations on human health (symptoms, therapeutics). It explains the phenomenology of criticality accidents, the prevention means, and the history of such accidents up to the Tokai-Mura one in 1999. Finally, the dosimetry of criticality is presented with its physical and biological techniques. (J.S.)

  16. Ecological implications of reduced pollen supply in the alpine: a case study using a dominant cushion plant species [v2; ref status: indexed, http://f1000r.es/3xc

    Directory of Open Access Journals (Sweden)

    Anya Reid

    2014-08-01

    Full Text Available The reproductive assurance hypothesis states that self-incompatible female plants must produce twice the number of seeds relative to their self-compatible hermaphroditic counterparts to persist in gynodioecious populations. This is a viable life-history strategy, provided that pollination rates are sufficiently high. However, reduced pollination rates in alpine plants are likely due to climate induced plant-pollinator mismatches and general declines in pollinators. Using a gynodioecious population of the dominant plant Silene acaulis (Caryophyllaceae, we tested the reproductive assurance hypothesis and also the stress gradient hypothesis with a series of pollinator exclusion trials and extensive measurements of subsequent reproductive output (gender ratio, plant size, percent fruit-set, fruit weight, seeds per fruit, total seeds, seed weight, and seed germination. The reproductive assurance hypothesis was supported with female plants being more sensitive to and less likely to be viable under reductions in pollination rates. These findings are the first to show that the stress gradient hypothesis is also supported under a gradient of pollen supply instead of environmental limitations. Beneficiary abundance was negatively correlated to percent fruit-set under current pollen supply, but became positive under reduced pollen supply suggesting that there are important plant-plant-pollinator interactions related to reproduction in these alpine plant species.

  17. Naturally-assisted metal phytoextraction by Brassica carinata: Role ofroot exudates

    International Nuclear Information System (INIS)

    Due to relatively high chelant dosages and potential environmental risks it is necessary to explore different approaches in the remediation of metal-contaminated soils. The present study focussed on the removal of metals (As, Cd, Cu, Pb and Zn) from a multiple metal-contaminated soil by growing Brassica carinata plants in succession to spontaneous metallicolous populations of Pinus pinaster, Plantago lanceolata and Silene paradoxa. The results showed that the growth of the metallicolous populations increased the extractable metal levels in the soil, which resulted in a higher accumulation of metals in the above-ground parts of B. carinata. Root exudates of the three metallicolous species were analysed to elucidate their possible role in the enhanced metal availability. The presence of metals stimulated the exudation of organic and phenolic acids as well as flavonoids. It was suggested that root exudates played an important role in solubilising metals in soil and in favouring their uptake by roots. - Phytoextraction of metals is enhanced in Brassica carinata grown in succession to metallicolous populations of spontaneous species.

  18. Dietary effects of four phytoecdysteroids on growth and development of the Indian meal moth, Plodia interpunctella.

    Science.gov (United States)

    Rharrabe, Kacem; Sayan, Fouad; Lafont, René

    2010-01-01

    Using pure phytoecdysteroids isolated from Ajuga iva (L.) Schreber (Lamiales: Lamiaceae) and Silene nutans L. (Caryophyllales: Caryophyllaceae), plants known for their high ecdysteroid content, a study was carried out on the effects of ingestion of four different phytoecdysteroids (20-hydroxyecdysone, polypodine B, ponasterone A and makisterone A) on the growth and development of the Indian meal moth, Plodia interpunctella Hübner (Lepidoptera: Pyralidae) larvae when added at a concentration of 200 ppm in their diet. The experiments clearly showed the susceptibility of P. interpunctella to phytoecdysteroid ingestion. The toxicity of phytoecdysteroids manifested itself by a decrease in larval weight, induction of cannibalism and an increase of mortality, together with disruption of development. The severity of the phytoecdysteroid effect on P. interpunctella depended on the structure of the molecule. The results demonstrate that the minimal structural differences existing between these four phytoecdysteroids significantly affected their toxicity toward P. interpunctella. Makisterone A was the most toxic of the four compounds towards P. interpunctella larvae. In conclusion, phytoecdysteroids ingestion evokes disruptive growth effects on P. interpunctella. This work supports a role for phytoecdysteroids in plant defence against phytophagous insects.

  19. LLNL Results from CALIBAN-PROSPERO Nuclear Accident Dosimetry Experiments in September 2014

    Energy Technology Data Exchange (ETDEWEB)

    Lobaugh, M. L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Hickman, D. P. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wong, C. W. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wysong, A. R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Merritt, M. J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Heinrichs, D. P. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Topper, J. D. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2015-05-21

    Lawrence Livermore National Laboratory (LLNL) uses thin neutron activation foils, sulfur, and threshold energy shielding to determine neutron component doses and the total dose from neutrons in the event of a nuclear criticality accident. The dosimeter also uses a DOELAP accredited Panasonic UD-810 (Panasonic Industrial Devices Sales Company of America, 2 Riverfront Plaza, Newark, NJ 07102, U.S.A.) thermoluminescent dosimetery system (TLD) for determining the gamma component of the total dose. LLNL has participated in three international intercomparisons of nuclear accident dosimeters. In October 2009, LLNL participated in an exercise at the French Commissariat à l’énergie atomique et aux énergies alternatives (Alternative Energies and Atomic Energy Commission- CEA) Research Center at Valduc utilizing the SILENE reactor (Hickman, et.al. 2010). In September 2010, LLNL participated in a second intercomparison at CEA Valduc, this time with exposures at the CALIBAN reactor (Hickman et al. 2011). This paper discusses LLNL’s results of a third intercomparison hosted by the French Institut de Radioprotection et de Sûreté Nucléaire (Institute for Radiation Protection and Nuclear Safety- IRSN) with exposures at two CEA Valduc reactors (CALIBAN and PROSPERO) in September 2014. Comparison results between the three participating facilities is presented elsewhere (Chevallier 2015; Duluc 2015).

  20. Detoxification of arsenic by phytochelatins in plants.

    Science.gov (United States)

    Schmöger, M E; Oven, M; Grill, E

    2000-03-01

    As is a ubiquitous element present in the atmosphere as well as in the aquatic and terrestrial environments. Arsenite and arsenate are the major forms of As intoxication, and these anions are readily taken up by plants. Both anions efficiently induce the biosynthesis of phytochelatins (PCs) ([gamma-glutamate-cysteine](n)-glycine) in vivo and in vitro. The rapid induction of the metal-binding PCs has been observed in cell suspension cultures of Rauvolfia serpentina, in seedlings of Arabidopsis, and in enzyme preparations of Silene vulgaris upon challenge to arsenicals. The rate of PC formation in enzyme preparations was lower compared with Cd-induced biosynthesis, but was accompanied by a prolonged induction phase that resulted finally in higher peptide levels. An approximately 3:1 ratio of the sulfhydryl groups from PCs to As is compatible with reported As-glutathione complexes. The identity of the As-induced PCs and of reconstituted metal-peptide complexes has unequivocally been demonstrated by electrospray ionization mass spectroscopy. Gel filtration experiments and inhibitor studies also indicate a complexation and detoxification of As by the induced PCs.

  1. НОВЫЕ ДЛЯ СИБИРИ И МАЛОИЗВЕСТНЫЕ ЧУЖЕРОДНЫЕ ВИДЫ РАСТЕНИЙ

    OpenAIRE

    Эбель, Александр

    2013-01-01

    В статье сообщаются сведения о распространении в Сибири 6 видов заносных растений, ранее не отмеченных для этой территории (Anthemis arvensis, Carduus hamulosus, Centaurea majorovii, Elytrigia intermedia, Papaver dubium, Physalis peruviana), 4 видов редких в Сибири ксенофитов (Cuscuta campestris, Erucastrum gallicum, Poa compressa, Silene dichotoma) и 4 видов, дичающих из культуры (Malva moschata, Pyrethrum parthenium, Symphytum caucasicum, Xanthoxalis corniculata)....

  2. Evaluation of native plant flower characteristics for conservation biological control of Prays oleae.

    Science.gov (United States)

    Nave, A; Gonçalves, F; Crespí, A L; Campos, M; Torres, L

    2016-04-01

    Several studies have shown that manipulating flowering weeds within an agroecosystem can have an important role in pest control by natural enemies, by providing them nectar and pollen, which are significant sources of nutrition for adults. The aim of this study was to assess if the olive moth, Prays oleae (Bernard, 1788) (Lepidoptera: Praydidae), and five of its main natural enemies, the parasitoid species Chelonus elaeaphilus Silvestri (Hymenoptera: Braconidae), Apanteles xanthostigma (Haliday) (Hymenoptera: Braconidae), Ageniaspis fuscicollis (Dalman) (Hymenoptera: Encyrtidae) and Elasmus flabellatus (Fonscolombe) (Hymenoptera: Eulophidae), as well as the predator Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae), can theoretically access the nectar from 21 flowering weeds that naturally occur in olive groves. Thus, the architecture of the flowers as well as the mouthpart structure and/or the head and thorax width of the pest and its enemies were analyzed. The results suggested that all beneficial insects were able to reach nectar of the plant species from Apiaceae family, i.e. Conopodium majus (Gouan) Loret, Daucus carota L. and Foeniculum vulgare Mill., as well as Asparagus acutifolius L., Echium plantagineum L., Capsella bursa-pastoris (L.) Medik., Raphanus raphanistrum L., Lonicera hispanica Boiss. et Reut., Silene gallica L., Spergula arvensis L., Hypericum perforatum L., Calamintha baetica Boiss. et Reut, Malva neglecta Wallr. and Linaria saxatilis (L.) Chaz. P. oleae was not able to access nectar from five plant species, namely: Andryala integrifolia L., Chondrilla juncea L., Dittrichia viscosa (L.) Greuter, Sonchus asper (L.) Hill and Lavandula stoechas L. PMID:26780918

  3. Natural and planted flora of the log mountain surface - mined demonstration area, Bell County, Kentucky

    Energy Technology Data Exchange (ETDEWEB)

    Thompson, R.L. [Berea College, KY (United States); Wade, G.L. [USDA Forest Service, Burlington, VT (United States); Straw, R.A. [Univ. of Tennessee Plateau Experiment Station, Crossville, TN (United States)

    1996-12-31

    A descriptive study of the naturally invading and planted flora was conducted during 1984-1985 on a 14- and 21-year-old contour surface mine the 14.2 ha Log Mountain Demonstration Area (LMDA), in Bell County, Kentucky. Six habitats are designated from areas created from coal mining; the 1963 bench, 1970 bench, bench highwalls, mine outslopes, mine seeps, and coal haul-telephone microwave tower road. Twenty-four of 25 woody and herbaceous species (11 indigenous, 13 non-indigenous) have persisted from plantings by personnel of the Northeastern Forest Experiment Station, USDA Forest Service. We recommend 11 native and exotic woody and herbaceous species for planting on coal surface-mined areas. An annotated list of vascular plants comprises 360 taxa (286 indigenous, 74 non-indigenous) in 224 genera from 82 families. Taxa consist of 1 Lycopodiophyta, 1 Equisetophyta, 8 Polypodiophyta, 7 Pinophyta, and 343 Magnoliophyta. The most species-rich families are the Asteraceae (64), Poaceae (39), Fabaceae (20), Cyperaceae (16), Rosaceae (13), and Lamiaceae (11). A total of 155 Bell County distribution records were documented. Three threatened Kentucky species (Gentiana decora, Liparis loeselii, Silene ovata) were present in refugial habitats created by surface mining. The high species richness has resulted from native and naturalized invading species from the environs, native and exotic planted species, and species from the remnant seed bank. Forest vegetation is a complex mosaic of natural and semi-natural plant communities on the unplanted and planted areas of LMDA.

  4. Extensions to Dynamic System Simulation of Fissile Solution Systems

    Energy Technology Data Exchange (ETDEWEB)

    Klein, Steven Karl [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Bernardin, John David [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Kimpland, Robert Herbert [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Spernjak, Dusan [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-08-24

    Previous reports have documented the results of applying dynamic system simulation (DSS) techniques to model a variety of fissile solution systems. The SUPO (Super Power) aqueous homogeneous reactor (AHR) was chosen as the benchmark for comparison of model results to experimental data for steadystate operation.1 Subsequently, DSS was applied to additional AHR to verify results obtained for SUPO and extend modeling to prompt critical excursions, ramp reactivity insertions of various magnitudes and rate, and boiling operations in SILENE and KEWB (Kinetic Experiment Water Boiler).2 Additional models for pressurized cores (HRE: Homogeneous Reactor Experiment), annular core geometries, and accelerator-driven subcritical systems (ADAHR) were developed and results reported.3 The focus of each of these models is core dynamics; neutron kinetics, thermal hydraulics, radiolytic gas generation and transport are coupled to examine the time-based evolution of these systems from start-up through transition to steady-state. A common characteristic of these models is the assumption that (a) core cooling system inlet temperature and flow and (b) plenum gas inlet pressure and flow are held constant; no external (to core) component operations that may result in dynamic change to these parameters are considered. This report discusses extension of models to include explicit reference to cooling structures and radiolytic gas handling. The accelerator-driven subcritical generic system model described in References 3 and 4 is used as a basis for this extension.

  5. Scale dependence of sex ratio in wild plant populations: implications for social selection.

    Science.gov (United States)

    Sanderson, Brian J; Augat, Malcolm E; Taylor, Douglas R; Brodie, Edmund D

    2016-03-01

    Social context refers to the composition of an individual's social interactants, including potential mates. In spatially structured populations, social context can vary among individuals within populations, generating the opportunity for social selection to drive differences in fitness functions among individuals at a fine spatial scale. In sexually polymorphic plants, the local sex ratio varies at a fine scale and thus has the potential to generate this opportunity. We measured the spatial distribution of two wild populations of the gynodioecious plant Silene vulgaris and show that there is fine-scale heterogeneity in the local distribution of the sexes within these populations. We demonstrate that the largest variance in sex ratio is among nearest neighbors. This variance is greatly reduced as the spatial scale of social interactions increases. These patterns suggest the sex of neighbors has the potential to generate fine-scale differences in selection differentials among individuals. One of the most important determinants of social interactions in plants is the behavior of pollinators. These results suggest that the potential for selection arising from sex ratio will be greatest when pollen is shared among nearest neighbors. Future studies incorporating the movement of pollinators may reveal whether and how this fine-scale variance in sex ratio affects the fitness of individuals in these populations. PMID:26865952

  6. Research Nuclear Reactors

    International Nuclear Information System (INIS)

    Published in English and in French, this large report first proposes an overview of the use and history of research nuclear reactors. It discusses their definition, and presents the various types of research reactors which can be either related to nuclear power (critical mock-ups, material test reactors, safety test reactors, training reactors, prototypes), or to research (basic research, industry, health), or to specific particle physics phenomena (neutron diffraction, isotope production, neutron activation, neutron radiography, semiconductor doping). It reports the history of the French research reactors by distinguishing the first atomic pile (ZOE), and the activities and achievements during the fifties, the sixties and the seventies. It also addresses the development of instrumentation for research reactors (neutron, thermal, mechanical and fission gas release measurements). The other parts of the report concern the validation of neutronics calculations for different reactors (the EOLE water critical mock-up, the MASURCA air critical mock-up dedicated to fast neutron reactor study, the MINERVE water critical mock-up, the CALIBAN pulsed research reactor), the testing of materials under irradiation (OSIRIS reactor, laboratories associated with research reactors, the Jules Horowitz reactor and its experimental programs and related devices, irradiation of materials with ion beams), the investigation of accident situations (on the CABRI, Phebus, Silene and Jules Horowitz reactors). The last part proposes a worldwide overview of research reactors

  7. Rhizosphere characteristics of indigenously growing nickel hyperaccumulator and excluder plants on serpentine soil

    International Nuclear Information System (INIS)

    Field study reinforces that root exudates may contribute to nickel hyperaccumulation in Thlaspi goesingense Halacsy. - The role of rhizosphere processes in metal hyperaccumulation is largely unexplored and a matter of debate, related field data are virtually not available. We conducted a field survey of rhizosphere characteristics beneath the Ni hyperaccumulator Thlaspi goesingense Halacsy and the metal-excluder species Silene vulgaris L. and Rumex acetosella L. growing natively on the same serpentine site. Relative to bulk soil and to the rhizosphere of the excluder species, we found significantly increased DOC and Ni concentrations in water extracts of T. goesingense rhizosphere, whereas exchangeable Ni was depleted due to excessive uptake of Ni. Chemical speciation analysis using the MINTEQA2 software package revealed that enhanced Ni solubility in Thlaspi rhizosphere is driven by the formation of Ni-organic complexes. Moreover, ligand-induced dissolution of Ni-bearing minerals is likely to contribute to enhanced Ni solubility. Increased Mg and Ca concentrations and pH in Thlaspi rhizosphere are consistent with ligand-induced dissolution of orthosilicates such as forsterite (Mg2SiO4). Our field data reinforce the hypothesis that exudation of organic ligands may contribute to enhanced solubility and replenishment of metals in the rhizosphere of hyperaccumulating species

  8. Сообщение о нескольких новых местонахождениях редких видов сосудистых растений на территории Ленинградской области и Санкт-Петербурга

    OpenAIRE

    Доронина, А.

    2008-01-01

    В статье приводятся сведения о новых местонахождениях 16 редких видов сосудистых растений на территории Ленинградской области и Санкт-Петербурга. Pulsatilla vernalis, Lycopodiella inundata, Dactylorhiza baltica, D. traunsteineri, Silene tatarica, Isatis tinctoria, Orobanche pallidiflora, Lathraea squamaria, Corallorhiza trifida, Botrychium lunaria—охраняемые виды; Potentilla bifurca, Conium maculatum—редкие адвентивные виды, Echium vulgare, Moneses uniflora, Galeobdolon luteum—редкие виды в в...

  9. TL detectors for gamma ray dose measurements in criticality accidents.

    Science.gov (United States)

    Miljanić, Saveta; Zorko, Benjamin; Gregori, Beatriz; Knezević, Zeljka

    2007-01-01

    Determination of gamma ray dose in mixed neutron+gamma ray fields is still a demanding task. Dosemeters used for gamma ray dosimetry are usually in some extent sensitive to neutrons and their response variations depend on neutron energy i.e., on neutron spectra. Besides, it is necessary to take into account the energy dependence of dosemeter responses to gamma rays. In this work, several types of thermoluminescent detectors (TLD) placed in different holders used for gamma ray dose determination in the mixed fields were examined. Dosemeters were from three different institutions: Ruder Bosković Institute (RBI), Croatia, JoZef Stefan Institute (JSI), Slovenia and Autoridad Regulatoria Nuclear (ARN), Argentina. All dosemeters were irradiated during the International Intercomparison of Criticality Accident Dosimetry Systems at the SILENE Reactor, Valduc, June 2002. Three accidental scenarios were reproduced and in each irradiation the dosemeters were exposed placed on the front of phantom and 'free in air'. Following types of TLDs were used: 7LiF (TLD-700), CaF2:Mn and Al2O3:Mg,Y-all from RBI; CaF2:Mn from JSI and 7LiF (TLD-700) from ARN. Reported doses were compared with the reference values as well as with the values obtained from the results of all participants. The results show satisfactory agreement with other dosimetry systems used in the Intercomparison. The influence of different types of holders and applied corrections of dosemeters' readings are discussed. PMID:17369267

  10. Inspecting the transformation of Roman settlements in the Upper Potenza Valley (Marche region across Late Antiquity and into the Early Medieval era

    Directory of Open Access Journals (Sweden)

    Francesca Carboni

    2015-12-01

    Full Text Available The following analysis shows the changes occurred in the settlement patterns in the upper Potenza river valley (MC, Marche region during the transition period between Late Antiquity and Early Middle Ages. This analysis is mainly based on the results of a geoarchaeological project, which has been carried out by a team from Ghent University since 2000. The review of the pottery collected during the fi eld survey has allowed for a better defined chronology of the last phase of occupation of the rural sites identifi ed in the sample zone, located within an intermediate basin between the Umbria-Marche Apennines and a lateral dorsal ridge, in areas dominated by the hilltops of Monte Primo and Monte Castel Santa Maria. For some of these sites, it is now possible to ascertain a continuity of life up to the end of the seventh century and further into the Middle Ages. La presente analisi illustra le trasformazioni delle modalità insediative avvenute nel periodo di transizione fra la tarda antichità e il medioevo nell’alta valle del fi ume Potenza (MC, Marche. Essa si basa sui risultati del progetto condotto con metodo geo-archeologico da un gruppo di ricerca dell’Università di Ghent, dal 2000. La revisione del materiale ceramico raccolto nel corso delle ricognizioni ha consentito di defi nire meglio le ultime fasi di occupazione dei siti rurali identifi cati nella zona campione in questione, posizionata all’interno del bacino intramontano posto fra l’Appennino umbro-marchigiano e una dorsale montuosa laterale, dominata dalle cime del Monte Primo e del Monte Santa Maria. Per alcuni di questi siti è stato, infatti, possibile accertare una continuità di occupazione estesa fi no al VII secolo e oltre, in età medievale.

  11. IL PROCESSO DI PRODUZIONE SCRITTA: LA COESIONE VERBALE IN TESTI DI SCUOLA PRIMARIA

    Directory of Open Access Journals (Sweden)

    Fabiana Rosi

    2015-02-01

    Full Text Available Una delle principali difficoltà che incontrano i bambini della scuola primaria nella produzione scritta riguarda la coesione verbale, ovvero il mantenimento lungo il testo della dimensione temporale inizialmente selezionata per riportare gli eventi. L’articolo esamina questo specifico tipo di competenza testuale negli alunni di sei classi del secondo ciclo della scuola primaria, tre delle quali hanno partecipato alla sperimentazione educativa sulla produzione di testi scritti “Osservare l’interlingua”. Il confronto fra i dati raccolti all’inizio e alla fine dell’anno scolastico mostra una riduzione dei salti immotivati da un tempo verbale all’altro, definiti shift temporali, nei testi prodotti nelle classi del campione sperimentale in misura maggiore rispetto a quelle di controllo e sottolinea gli effetti positivi, a breve e lungo termine, di una didattica task-based condotta con approccio inclusivo, cooperativo ed attivo.  The process of writing: verb cohesion in primary school textsOne of principal difficulties in producing written texts for primary school pupils concerns verb cohesion, i.e. maintaining the temporal dimension initially chosen for expressing events throughout the text. The paper examines this specific textual competence in pupils from six classes within second cycle of primary school. Three of these classes were involved in the “Observing interlanguage” project focused on written production. The comparison of data collected at the beginning and the end of school year shows a decrease in temporal shifts (transitions from one verb tense to another in the texts produced by the students involved in the project with regard to the control group. These findings highlight the positive effects, both short-term and long-term, of task-based teaching through an inclusive, cooperative and active approach. 

  12. The Causality of Casualness in the Translations of World Poetry: Jorie Graham vs Mary Oliver in Italy

    Directory of Open Access Journals (Sweden)

    Paola Loreto

    2014-10-01

    Full Text Available The essay explores the impact of poetry translation on the circulation of the works of American poets in Italy. The research is conducted by means of a double approach: in the first place, the aesthetic and cultural value of the poetry of two American women poets (Jorie Graham and Mary Oliver is assessed, whose work has received a radically different reception. Secondly, the reasons for this disparity of treatment are sought in the interrelation between the practices and activity of poetry translation and of publication, as they are customarily led in Italy. These are inferred from sample interviews with professionals in both fields. The results of the investigation highlight a combination of the casual and the causal, the causing factor being the occasional encounter of a poet with a translator, who then chooses his/her author, texts, and translating strategies according to his/her personal aesthetic, and sometimes ideological, judgment. Il saggio offre un’analisi dell’influenza delle traduzioni sulla circolazione della poesia americana contemporanea in Italia. La ricerca si articola in due momenti: in primo luogo viene fornita una valutazione estetica e culturale dell’opera di due poetesse americane, Jorie Graham e Mary Oliver, la cui ricezione in Italia è particolarmente significativa perché sta conoscendo fortune opposte; in secondo luogo viene condotta un’indagine dei motivi di questa disparità di trattamento nell’interazione tra le pratiche di traduzione e le dinamiche di pubblicazione in atto in Italia. Queste sono state dedotte da alcune interviste campione con professionisti dei due campi. I risultati hanno evidenziato una combinazione di fattori casuali e causali, nella quale l’incontro personale e a volte fortuito tra un poeta e un traduttore mette in moto strategie di selezione e di traduzione motivate da ragioni estetiche e a volte ideologiche. 

  13. A strategy of fox management in Italy: the guide lines of the Istituto Nazionale di Biologia della Selvaggina / Proposte per una strategia nazionale di gestione della Volpe: le linee direttrici dell'Istituto Nazionale di Biologia della Selvaggina

    Directory of Open Access Journals (Sweden)

    Silvano Toso

    1991-07-01

    Full Text Available Abstract Authors critically consider the usual fox management in Italy and suggest a new strategy concerning the hunting activity and population control. The following topics are discussed: a improvement of the knowledge about the geographical and ecological distribution of foxes using the information furnished by a specific national atlas; b population dynamics monitoring mainly obtained from the bag records and the cheking of breeding dens in selected sampling areas; c hunting methods and season; d guide-lines for the policy of fox control based on an up-to-date review about the effects of fox predation on game and the effectiveness of the different methods carried on. Riassunto Gli Autori esaminano criticamente il tipo di gestione cui viene sottoposta la Volpe Vulpes vulpes in Italia e suggeriscono una nuova strategia per ciò che concerne il prelievo venatorio ed il controllo delle popolazioni. In particolare vengono affrontati i seguenti temi: a miglioramento delle conoscenze sulla distribuzione geografica ed ecologica della specie attraverso la redazione di uno specifico atlante nazionale; b monitoraggio della dinamica delle popolazioni, ottenuto soprattutto con l'analisi dei carnieri ed il conteggio delle tane riproduttive in aree campione; c tempi e modalità del prelievo venatorio; d analisi delle attuali conoscenze circa l'impatto della predazione della Volpe sulla selvaggina e conseguenti indicazioni sull'opportunità e efficacia delle operazioni di controllo delle popolazioni volpine anche in relazione ai metodi diretti ed indiretti utilizzati.

  14. Fungistatic activity of all-trans retinoic acid against Aspergillus fumigatus and Candida albicans

    Directory of Open Access Journals (Sweden)

    Campione E

    2016-04-01

    Full Text Available Elena Campione,1 Roberta Gaziano,2 Daniele Marino,2 Augusto Orlandi3 1Department of Dermatology, 2Department of Microbiology, 3Department of Anatomic Pathology, University of Rome Tor Vergata, Rome, Italy Purpose: Fungal infections are a major complication in hematologic and neoplastic patients causing severe morbidity and mortality. Aspergillus fumigatus and Candida albicans are among the most invasive opportunistic pathogens in immunocompromised patients, and classic antifungal drugs are frequently unsuccessful in these patients. Recent reports hypothesize that the antifungal efficacy of all-trans retinoic acid (ATRA is mainly related to its strong capacity to stimulate monocyte-mediated immunity, but no consideration was given to its potential direct fungistatic activity. Moreover, ATRA offers the opportunity for systemic therapy. Methods and results: We investigated the efficacy of ATRA at different concentrations for its antifungal activity against opportunistic A. fumigatus and C. albicans obtained from clinical samples according to standard protocols. A fungistatic activity of ATRA on A. fumigatus and C. albicans at 0.5–1 mM concentration was documented up to 7 days. Conclusion: This is the first evidence of a direct and strong fungistatic activity of ATRA against A. fumigatus and C. albicans. The potential adjuvant therapeutic application of ATRA might be useful in the treatment and/or prevention of systemic mycoses in immunocompromised patients. The discovery of a direct fungistatic activity, in association with its reported immunomodulatory properties, makes ATRA an excellent candidate for new combined antifungal strategies for systemic mycoses in immunocompromised and cancer patients. Keywords: all-trans retinoic acid, fungistatic activity, fungal infections

  15. Prevalenza di anticorpi anti-Legionella in gruppi di operatori odpedalieri provenienti da due regioni italiane

    Directory of Open Access Journals (Sweden)

    G. Stancanelli

    2003-05-01

    Full Text Available

    Obiettivi: é stato condotto uno studio di prevalenza per stabilire la presenza di anticorpi anti- Legionella in persone che svolgono diverse attività lavorative, tenendo conto dei fattori personali e ambientali che possono associarsi ad un maggior rischio espositivo.

    Metodi: sono state esaminate 160 persone di entrambi i sessi, suddivisi in base alla professione in operatori sanitari (n.52, operai (n.55 e impiegati (n.53. Dopo l’adesione tramite consenso informato, per ciascun soggetto veniva compilato un apposito questionario per indagare sui fattori di rischio per l’esposizione a Legionella spp e prelevato un campione di sangue per la ricerca sul siero di anticorpi IgG anti-Legionella (metodo IFA

    Risultati: il 43,8% dei medici e il 33,3% degli infermieri sono risultati positivi (titolo›1:128, contro il 16,4% degli operai e il 13,2 degli impiegati (10,55, p‹0,05. I gruppi più frequenti erano L. pneumophila sierogruppo 7-14 (77,1% dei positivi e L. non pneumophila, L’esame dei fattori di rischio non ha evidenziato associazioni significative con sesso, fumo, bevande alcoliche, anzianità lavorativa ed altre caratteristiche personali. I sintomi simil-influenzali nell’ultimo anno e gli episodi bronco-polmonari riferibili agli ultimi 10 anni non si associavano ad un maggior numero e/o grado di positività.

    Conclusioni: la sieropositività per Legionella è risultata associata con lo svolgimento delle mansioni di medico e/o infermiere ad indicare una maggior esposizione a Legionella spp negli ambienti sanitari, ma nella maggior parte dei casi si trattava di anticorpi verso i sierogruppi a bassa espressività clinica, come dimostrato dalla assenza anamnestica di legionellosi nel gruppo allo studio.

  16. I PANNELLI DI SALA DEL MUSEO DEL 900: CARATTERISTICHE LINGUISTICHE E TESTUALI

    Directory of Open Access Journals (Sweden)

    Elena Villa

    2016-02-01

    Full Text Available Il lavoro cerca di analizzare le caratteristiche linguistiche e testuali dei pannelli di sala dei Musei. L’analisi è stata svolta seguendo un approccio di tipo orizzontale. Il testo è stato indagato secondo una modalità gerarchica considerando i  quattro livelli di analisi caratteristici dei linguaggi settoriali: la morfologia, la sintassi, il lessico e la testualità.Sono stati esaminati casi particolari di pannelli esplicativi, cioè quelli che introducono alle prime sei sale del Museo del Novecento di Milano; inoltre sono stati forniti degli esempi concreti a supporto dell’analisi linguistica e la trascrizione effettiva dei testi presi in esame al fine di dare maggiore esaustività al lavoro.Si è infine sottoposto il campione testuale scelto a due classi di studenti universitari stranieri appartenenti ai progetti Socrates Erasmus e Marco Polo, per testarne l’effettiva comprensibilità da parte di quelli che potrebbero essere potenziali visitatori. Gallery panels in the Museo del 900: linguistic and textual featuresThe paper analyzes the linguistic and textual gallery panels at the Museo del 900. The analysis was carried out using a horizontal approach. The text was investigated considering the four hierarchical levels of analysis for specialized language: morphology, syntax, vocabulary and textuality. The explanatory gallery panels that introduce the first six halls of the Museo del Novecento in Milan were examined. Specific examples support the linguistic analysis as well as the actual transcript of the texts examined. The sample text was submitted to two groups of foreign university students (participants in the Socrates Erasmus and Marco Polo projects in order to test its understandability by subjects who could be potential visitors.

  17. Indagine sulle opinioni, conoscenze ed attitudini dei docenti scolastici della provincia di Frosinone in tema di tossicodipendenza

    Directory of Open Access Journals (Sweden)

    M. Ferrara

    2003-05-01

    Full Text Available

    Obiettivi: lo studio in questione mirava a valutare le conoscenze circa gli effetti e l’uso personale del fumo di tabacco e delle droghe in generale in un campione di insegnanti delle Scuole Medie Superiori della provincia di Frosinone.

    Metodi: sono stati somministrati questionari anonimi a 350 docenti frequentanti un Corso di Aggiornamento sulle tossicodipendenze.

    Risultati: il 23.4% ha dichiarato di essere fumatore, con un consumo medio pro die di 10 sigarette: di questi la maggior parte era di sesso femminile. Sul fastidio che si prova in presenza di un fumatore il 54.6% ha risposto positivamente, tra cui il 14.6% sono fumatori. Il 94% dichiara di non aver utilizzato alcun tipo di sostanza mentre il 5.5%, tra cui in prevalenza femmine, dichiara di utilizzare psicofarmaci. Le sostanze stupefacenti ritenute più pericolose sono eroina 82.9%, cocaina 81.1%, allucinogeni 70%, canapa indiana 54.9%; alcol 58% e tabacco 18% sono meno pericolose. Il 15.7% ritiene che l’abitudine al fumo sia scatenata dalla socializzazione, il 15.1% dall’imitazione, il 15.4% dal piacere e dalla curiosità, l’11.1% dal sentirsi grandi, l’8% dal gusto del proibito. Il 97.1% mostra un adeguato livello di conoscenza sulle droghe e il cervello è il principale organo danneggiato (96.3%.

    Conclusioni: la conoscenza dei docenti in materia di tossicodipendenze sembra concentrata sulle droghe illegali: infatti, i rischi legati al consumo di alcol e tabacco sono sottovalutati e non sono chiari i fattori determinanti la dipendenza. Infine i docenti hanno espresso l’esigenza di aggiornamento per l’insufficiente opera di informazione e prevenzione svolta da parte delle istituzioni competenti.

  18. [Empathy in the nurse practitioners of medical area].

    Science.gov (United States)

    Facco, Simona; Cirio, Luigi; Galante, Jenni; Dimonte, Valerio

    2014-01-01

    Introduzione. Attualmente sono pochi gli studi che indagano la capacità empatica nella popolazione infermieristica. Obiettivo di questo studio è analizzare la capacità empatica degli infermieri che prestano servizio in area medica in relazione a genere, età, formazione e anzianità di servizio. Materiali e metodi. Lo studio è di tipo descrittivo-osservazionale, su un campione di 60 infermieri. Lo strumento utilizzato per la valutazione dell’empatia è la scala di valutazione JSPE (versione HP) costituita da 20 item, in cui gli intervistati indicano il loro accordo o disaccordo con una scala Likert a 7 punti. Il range di valori possibili è da 20 a 140: il punteggio più alto corrisponde a una maggiore capacità empatica. Il questionario ha dimostrato la validità e l'affidabilità della versione italiana del JSPE con un coefficiente alfa di Cronbach di 0,85. Il punteggio Il livello di significatività statistica accettato è pari a P ≤ 0,05. Risultati. La media della capacità empatica dei professionisti intervistati è risultata di 104/140. Dai dati analizzati, emerge che con l’aumentare degli anni di servizio alla professione si verifica una diminuzione dell’empatia così come il permanere nello stesso reparto provoca una diminuzione empatica. Inoltre è emersa una maggiore empatia degli infermieri della neurologia rispetto alla medicina generale. Conclusioni. Lo studio evidenzia come i professionisti abbiano necessità di incrementare la capacità empatica soprattutto col trascorrere degli anni di esercizio alla professione. Probabilmente come meccanismo di difesa, il professionista tende a proteggersi dal coinvolgimento emotivo, favorendo le attività di natura più tecnico-gestuale, piuttosto che relazionale.

  19. studio descrittivo delle evidenze neuropsicologiche in un gruppo di pazienti HIV positivi afferenti al reparto malattie infettive dell’ospedale di Alessandria

    Directory of Open Access Journals (Sweden)

    Amendola G.

    2012-01-01

    Full Text Available Obiettivi: Le ormai note complicanze neurologiche nell’infezione da HIV comportano la necessità di introdurre la figura del neuropsicologo nel team dedicato, con l’obiettivo iniziale di definire un modello di presa in carico per l’utenza e descriverne le caratteristiche e i bisogni rilevati.Metodologia: data la particolare caratterizzazione sottocorticale del danno neuro-cognitivo secondario a HIV, che interessa in modo trasversale un po’ tutti i sistemi neurocognitivi, è stato necessario ricorrere, sul campione iniziale che qui descriviamo, composto da 31 pazienti, ad un’ampia batteria di prove neuropsicologiche, in linea con i protocolli internazionali, completata da un questionario di valutazione dell’umore (Beck Depression Inventory e un’intervista sulla tipologia, qualità e livelli di soddisfazione all’interno dei principali legami di appartenenza.Risultati: i dati fino oggi raccolti hanno mostrato specifiche difficoltà a livello di funzioni esecutive. Alcuni indici di confronto, che dovranno essere sottoposti a successive conferme e più fini analisi, sono risultati suggestivi per la possibilità di individuare e selezionare, tra quelli comunemente usati in questo campo, alcuni paradigmi neuropsicologici dotati della più alta validità di costrutto e di specificità predittiva. Conclusioni: le azioni neuropsicologiche sembrano poter rappresentare, oltre ché un fattore preventivo per i pazienti con HIV, una verifica ed eventuale adeguamento dei livelli di aderenza alle terapie, utile al fine di ottimizzare le risorse. Garantiscono inoltre, grazie all’offerta di un possibile “contenitore” dato dallo specialista, di ridurre il gravoso carico emotivo della malattia.

  20. The determination of cadmium, lead and vanadium by high resolution ICP-MS in Antarctic snow samples

    Energy Technology Data Exchange (ETDEWEB)

    Lanza, F. [Joh. Gutenberg Univ., Mainz (Germany). Inst. fuer Anorganische und Analytische Chemie; Trincherini, P. [European Union Joint Research Centre, Ispra, VA (Italy). Inst. of Environment

    2000-02-01

    Double focusing ICP-MS was successfully used in this research for the direct determination of the cadmium, lead and vanadium content of a set of Antarctic samples collected during the 11. Italian Expedition to Antarctica. For cadmium and lead measurements the low resolution mode was chosen since it ensured the highest ion sensitivity and it allowed the highest detection limits to be attained; in the case of vanadium the high resolution mode was used to solve the isobaric interference. As far as vanadium and cadmium are concerned by GFAAS coupled with different preconcentration procedures. The vanadium, cadmium and lead content measured in a riverine water reference material (SRLS-3) was found in agreement with the certified values. [Italian] Si e' utilizzato uno spettrometro ICP-MS a doppia focalizzazione per la determinazione diretta di cadmio, piombo e vanadio in una serie di campioni di neve antartica superficiale raccolta durante l'undicesima spedizione italiana in Antartide. Per le determinazioni di cadmio e piombo si e' scelta la procedura a bassa risoluzione, che ha garantito la piu' elevata sensibilita' ed ha permesso di ottenere limiti di determinazione migliori; nel caso del vanadio si e' utilizzata la procedura ad alta risoluzione che ha permesso di risolvere problemi di interferenza isobarica. Per quanto riguarda cadmio e vanadio, i risultati delle determinazioni mediante ICP-MS sono risultati in buon accordo con quelli ottenuti mediante GFAAS accoppiata a diverse procedure di preconcentrazione. Il contenuto di cadmio, piombo e vanadio misurato con le procedure sviluppate in un campione certificato di acqua di fiume (SRLS-3) e' risultato in accordo con i valori certificati.

  1. Initial investigation into computer scoring of candidate essays for personnel selection.

    Science.gov (United States)

    Campion, Michael C; Campion, Michael A; Campion, Emily D; Reider, Matthew H

    2016-07-01

    [Correction Notice: An Erratum for this article was reported in Vol 101(7) of Journal of Applied Psychology (see record 2016-32115-001). In the article the affiliations for Emily D. Campion and Matthew H. Reider were originally incorrect. All versions of this article have been corrected.] Emerging advancements including the exponentially growing availability of computer-collected data and increasingly sophisticated statistical software have led to a "Big Data Movement" wherein organizations have begun attempting to use large-scale data analysis to improve their effectiveness. Yet, little is known regarding how organizations can leverage these advancements to develop more effective personnel selection procedures, especially when the data are unstructured (text-based). Drawing on literature on natural language processing, we critically examine the possibility of leveraging advances in text mining and predictive modeling computer software programs as a surrogate for human raters in a selection context. We explain how to "train" a computer program to emulate a human rater when scoring accomplishment records. We then examine the reliability of the computer's scores, provide preliminary evidence of their construct validity, demonstrate that this practice does not produce scores that disadvantage minority groups, illustrate the positive financial impact of adopting this practice in an organization (N ∼ 46,000 candidates), and discuss implementation issues. Finally, we discuss the potential implications of using computer scoring to address the adverse impact-validity dilemma. We suggest that it may provide a cost-effective means of using predictors that have comparable validity but have previously been too expensive for large-scale screening. (PsycINFO Database Record PMID:27077525

  2. L’ITALIANO ALL’UNIVERSITÉ DE SAVOIE

    Directory of Open Access Journals (Sweden)

    Andrea Costantini

    2014-02-01

    Full Text Available Il lavoro traccia un profilo degli studenti iscritti al corso di laurea triennale in Lingua, letteratura e cultura italiana della facoltà di Lettere, lingue e scienze umane dell’Université de Savoie (Chambéry, Francia. Basandosi su informazioni raccolte presso un campione di ventisei studenti tramite un apposito questionario, l’articolo delinea un ritratto degli apprendenti e ne analizza le esperienze passate e presenti di apprendimento linguistico; i contatti che essi hanno con l’Italia e con parlanti italofoni; le motivazioni di ordine culturale, professionale e personale che li hanno spinti all’apprendimento della lingua italiana. A partire dal loro profilo che i dati fanno emergere si stabilisce un confronto, in merito alle dinamiche di studio e alle motivazioni all’apprendimento dell’italiano, tra gli studenti di Chambéry e l’utenza dei corsi di lingua italiana organizzati dagli Istituti italiani di cultura nel mondo.  Italian at the Université de Savoie The article profiles students enrolled in the undergraduate program in Italian language, literature and culture at the Faculty of Arts, Languages and Humanities at the Université de Savoie (Chambéry, France. Based on information gathered from a sample of twenty-six students through a questionnaire, the article outlines a portrait of the learners and analyzes their past and present experience regarding language learning, contact they have with Italy and Italian speakers and the cultural, professional and personal reasons that brought them to study the Italian language. Based on the data, a comparison regarding the dynamics of Italian language learning and motivation among students in Chambéry and users of Italian language courses organized by the Italian Cultural Institutes in the world can be made.

  3. Urban schools' teachers enacting project-based science

    Science.gov (United States)

    Tal, Tali; Krajcik, Joseph S.; Blumenfeld, Phyllis C.

    2006-09-01

    What teaching practices foster inquiry and promote students to learn challenging subject matter in urban schools? Inquiry-based instruction and successful inquiry learning and teaching in project-based science (PBS) were described in previous studies (Brown & Campione, [1990]; Crawford, [1999]; Krajcik, Blumenfeld, Marx, Bass, & Fredricks, [1998]; Krajcik, Blumenfeld, Marx, & Solloway, [1994]; Minstrell & van Zee, [2000]). In this article, we describe the characteristics of inquiry teaching practices that promote student learning in urban schools. Teaching is a major factor that affects both achievement of and attitude of students toward science (Tamir, [1998]). Our involvement in reform in a large urban district includes the development of suitable learning materials and providing continuous and practiced-based professional development (Fishman & Davis, in press; van Es, Reiser, Matese, & Gomez, [2002]). Urban schools face particular challenges when enacting inquiry-based teaching practices like those espoused in PBS. In this article, we describe two case studies of urban teachers whose students achieved high gains on pre- and posttests and who demonstrated a great deal of preparedness and commitment to their students. Teachers' attempts to help their students to perform well are described and analyzed. The teachers we discuss work in a school district that strives to bring about reform in mathematics and science through systemic reform. The Center for Learning Technologies in Urban Schools (LeTUS) collaborates with the Detroit Public Schools to bring about reform in middle-school science. Through this collaboration, diverse populations of urban-school students learn science through inquiry-oriented projects and the use of various educational learning technologies. For inquiry-based science to succeed in urban schools, teachers must play an important role in enacting the curriculum while addressing the unique needs of students. The aim of this article is to

  4. Reflections About the Role of Intellectuals in Latin America Reflexiones acerca del rol de los intelectuales en América Latina Reflexões sobre o papel dos intelectuais na América Latina

    Directory of Open Access Journals (Sweden)

    Judith Naidorf

    2010-10-01

    Full Text Available

    The purpose of this paper is to address some theoretical aspects - concepts that contribute to reflection on the specific role of the current Latin American intellectual. The contributions of various authors will help us to characterize a role that is constantly rethinked, analyzed and disputed.

    The proposal is to retake the theoretical developments in the category "intellectual" from Antonio Gramsci and Immanuel Wallerstein mainly, but making reference to its most intense moments in the 60-70 decades with Sartre and Foucault in Europe. It also highlights the contributions made by Pierre Bourdieu, Sousa Santos and the contributions to Latin America by Fals Borda, Florestan Fernandez, Octavio Ianni and Eliseo Veron, among others. Also included are examples of statements for the EZLN by Subcomandante Marcos and refers to the analysis performed by Julio Gambina and Daniel Campione for the Argentine case.

    It’s not the interest of this work to arrive at definitions finished about the social function of

  5. Effect of Iron Plaque on Root Growth and Activity of Two Wetland Plants%根表铁膜对2种景观湿地植物根系发育及活力的影响

    Institute of Scientific and Technical Information of China (English)

    钟顺清

    2015-01-01

    Root activity is an important indicator of plant response to environmental conditions.High iron content (Fe-plaques)frequently form on roots of wetland plants,reflecting the adaptation of wetland plants to submersion, facilitates nutrient uptake and prevents uptake of phytotoxins.In this study,root activity and the growth of adventi-tious roots of Typha latifolia and Iris pseudacorus in solution culture experiments were determined to investigate the response of wetland plant roots to iron plaque induced on the root surface.Typha latifolia and Iris pseudacorus (each 60 plants)were cultivated in Hoagland nutrient solution for one week.After adventitious roots of Typha lati-folia and Iris pseudacorus emerged,the existing root was removed and the plants were cultivated in the Hoagland so-lution for an additional three weeks at a pH of 5 .5 .Thirty-five plants with similar shoot height and root length were transferred to seven plastic buckets with a solution pH of 5.0 and different Fe2+(FeSO4·7H2O)concentrations (0,10,20,60,100,150,200 mg/L)to induce iron plague formation.The buckets were covered with black po-lyethylene bags and filled with N2 gas.After five days,two plants were removed from solution to determine root ac-tivity.One week later,the fresh weight and activity of the adventitious roots of both species in all treatment groups were measured.Adventitious root growth of Typha latifolia was inhibited by Fe2+concentrations of less than 100 mg/L,but was promoted at concentrations over 100 mg/L.At higher Fe2+concentrations,the root activity of Typha latifolia decreased and there was a large difference [9.70 μg/(g·h)]between maximum and minimum values.However,the influence of Fe plague on the adventitious root growth of Iris pseudacorus was not significant in all treatments,and the root activity of Iris pseudacorus ranged from 2.36 μg/(g·h)to 5.48 μg/(g·h)with little variation among treatments.There was a significant negative relationship between the root

  6. Hot wire chemical vapor deposition chemistry in the gas phase and on the catalyst surface with organosilicon compounds.

    Science.gov (United States)

    Shi, Yujun

    2015-02-17

    CONSPECTUS: Hot wire chemical vapor deposition (HWCVD), also referred to as catalytic CVD (Cat-CVD), has been used to produce Si-containing thin films, nanomaterials, and functional polymer coatings that have found wide applications in microelectronic and photovoltaic devices, in automobiles, and in biotechnology. The success of HWCVD is largely due to its various advantages, including high deposition rate, low substrate temperatures, lack of plasma-induced damage, and large-area uniformity. Film growth in HWCVD is induced by reactive species generated from primary decomposition on the metal wire or from secondary reactions in the gas phase. In order to achieve a rational and efficient optimization of the process, it is essential to identify the reactive species and to understand the chemical kinetics that govern the production of these precursor species for film growth. In this Account, we report recent progress in unraveling the complex gas-phase reaction chemistry in the HWCVD growth of silicon carbide thin films using organosilicon compounds as single-source precursors. We have demonstrated that laser ionization mass spectrometry is a powerful diagnostic tool for studying the gas-phase reaction chemistry when combined with the methods of isotope labeling and chemical trapping. The four methyl-substituted silane molecules, belonging to open-chain alkylsilanes, dissociatively adsorb on W and Ta filaments to produce methyl radical and H2 molecule. Under the typical deposition pressures, with increasing number of methyl substitution, the dominant chemistry occurring in the gas phase switches from silylene/silene reactions to free-radical short chain reactions. This change in dominant reaction intermediates from silylene/silene to methyl radicals explains the observation from thin film deposition that silicon carbide films become more C-rich with a decreasing number of Si-H bonds in the four precursor molecules. In the case of cyclic monosilacyclobutanes, we have

  7. Seed release in serotinous lodgepole pine forests after mountain pine beetle outbreak.

    Science.gov (United States)

    Teste, François P; Lieffers, Victor J; Landhausser, Simon M

    2011-01-01

    There are concerns that large-scale stand mortality due to mountain pine beetle (MPB) could greatly reduce natural regeneration of serotinous Rocky Mountain (RM) lodgepole pine (Pinus contorta var. latifolia) because the closed cones are held in place without the fire cue for cone opening. We selected 20 stands (five stands each of live [control], 3 years since MPB [3-yr-MPB], 6 years since MPB [6-yr-MPB], and 9 years since MPB [9-yr-MPB] mortality) in north central British Columbia, Canada. The goal was to determine partial loss of serotiny due to fall of crown-stored cones via breakage of branches and in situ opening of canopy cones throughout the 2008 and 2009 growing seasons. We also quantified seed release by the opening of forest-floor cones, loss of seed from rodent predation, and cone burial. Trees killed by MPB three years earlier dropped approximately 3.5 times more cones via branch breakage compared to live stands. After six years, MPB-killed stands had released 45% of their canopy seed bank through cone opening, cone fall due to breakage, and squirrel predation. Further losses of canopy seed banks are expected with time since we found 9-yr-MPB stands had 38% more open canopy cones. This was countered by the development of a modest forest-floor seed bank (6% of the original canopy seed bank) from burial of cones; this seed bank may be ecologically important if a fire or anthropogenic disturbance reexposes these cones. If adequate levels of regeneration are to occur, disturbances to create seedbeds must occur shortly after tree mortality, before the seed banks are lost. Our findings also suggest that the sustained seed rain (over at least nine years) after MPB outbreak may be beneficial for population growth of ground-foraging vertebrates. Our study adds insight to the seed ecology of serotinous pines under a potentially continental-wide insect outbreak, threatening vast forests adapted to regeneration after fire. Key words: biotic disturbance; cone

  8. Distribución de plantas vasculares a escala local. Taxones con mayor ocupación geográfica en Ambite y Utande (La Alcarria Occidental

    Directory of Open Access Journals (Sweden)

    García-Abad Alonso, Juan Javier

    2016-06-01

    Full Text Available The method for performing Floral Cover Indices (FCI, presented in a previous article, is applied to two 100 km2 U.T.M. grid cells (30TVK86, Ambite and 30TWL02,Utande. They are located in different sectors of the natural region of Western “La Alcarria”. The goal is to know and compare distributions of presence and abundance of vascular plants at local scale. Geographical factors affecting cover of the flora is widely exposed. Analysis method and chorological maps drawing with four spatial levels (1, 4, 25 and 100 km2 are presented. Results of the twelve taxa with the highest FCI in the set of two grid cells and phytogeographical interpretations are shown: Genista scorpius, Quercus ilex subsp. ballota, Thymus vulgaris, Helianthemum cinereum subsp. rotundifolium, Brachypodium retusum, Thymus zygis subsp. sylvestris, Lavandula latifolia, Dactylis hispanica, Rosmarinus officinalis, Koeleria vallesiana, Quercus faginea y Salvia lavandulifolia.Se aplica la Metodología de Índices de Ocupación de la Flora, presentada en un artículo anterior, en dos cuadrículas de 100 km2 pertenecientes a La Alcarria Occidental, pero localizadas en sectores diferentes (Ambite, 30TVK86, y Utande, 30TWL02, con objeto de conocer y comparar las distribuciones geográficas de la presencia y abundancia de plantas vasculares a escala local. Se exponen ampliamente los factores geográficos diferenciales que inciden en la ocupación de la flora. Se presentan el método de análisis y la elaboración de la cartografía corológica para cuatro resoluciones cartográficas diferentes (1, 4, 25 y 100 km2. Se muestran y se comentan fitogeográficamente los resultados de los doce taxones con mayor IOP en el conjunto de las dos cuadrículas: Genista scorpius, Quercus ilex subsp. ballota, Thymus vulgaris, Helianthemum cinereum subsp. rotundifolium, Brachypodium retusum, Thymus zygis subsp. sylvestris, Lavandula latifolia, Dactylis hispanica, Rosmarinus officinalis, Koeleria

  9. Treatment of Marine-Aquaculture Effluent by the Multi-Soil-Layer (MSL) System and Subsurface Flow Constructed Wetland%多介质土壤滤层系统(MSL)与潜流式人工湿地技术处理海水养殖废水的效能比较

    Institute of Scientific and Technical Information of China (English)

    宋颖; 黄玉婷; 葛川; 张浩; 陈昕; 张志剑; 罗安程

    2014-01-01

    To evaluate the feasibility of using multi-soil-layer ( MSL ) system and subsurface flow constructed wetland to treat the wastewater of marine cultured Penaeus vannamei and to determine the suitable process for the local aquaculture wastewater pollution characteristics. In this study, MSL system and four constructed wetland systems with Spartina anglica, Phragmites australis, Typha latifolia and unplanted system were evaluated for their potentials of pollutants removal capacity. The results showed the average removal rates of chemical oxygen demand (COD), total phosphorus (TP), total nitrogen (TN), ammonia nitrogen (NH4+-N) and nitrate ( NO3--N) by MSL system were 80. 38℅ ± 2. 14℅, 68. 14℅ ± 3. 51℅, 40. 79℅ ± 3. 10℅, 42. 68℅ ± 2. 90℅ and 54. 19℅ ± 5. 15℅, respectively. Additionally, the ability of pollutants removal of other four wetland systems decreased in the order: Spartina anglica, Phragmites australis, Typha latifolia and unplanted system.%为探索经济可行的海水养殖废水处理技术,选取南美白对虾海水养殖废水,对比研究了多介质土壤滤层( multi-soil-layer, MSL)系统和4种不同植物配置潜流式人工湿地(大米草型、芦苇型、香蒲型和无植物型)在400 L•( m2•d)-1水力负荷下的废水处理效果.结果表明, MSL对化学需氧量( COD)、总磷( TP)、总氮( TN)、氨氮( NH4+-N)和硝态氮( NO3--N)的平均去除率分别为80.38℅±2.14℅、68.14℅±3.51℅、40.79℅±3.10℅、42.68℅±2.90℅和54.19℅±5.15℅,均高于其他4种不同植物配置的潜流式人工湿地的处理效果.4种人工湿地系统对各污染物的去除率从高到低依次为:大米草型﹥芦苇型﹥香蒲型﹥无植物型.

  10. Absorção de água pelas raízes do limoeiro sob distintas freqüências de irrigação Water uptake by lemon roots under different irrigation frequencies

    Directory of Open Access Journals (Sweden)

    Delfran B. dos Santos

    2005-09-01

    Full Text Available Objetivou-se, nesse trabalho, caracterizar a absorção de água pelas raízes do limoeiro 'Taiti' (Citrus latifolia Tanaka no perfil de solo sob diferentes intervalos de irrigação por microaspersão. O estudo foi realizado em condições semi-áridas, em solo franco arenoso, cuja absorção de água pelas raízes das plantas foi determinada a partir da umidade em diversas posições no perfil do solo entre duas irrigações, por meio de sondas de TDR. A distribuição radicular foi avaliada a partir de dados de comprimento e diâmetro das raízes nas mesmas posições das sondas de TDR. Conforme os resultados, verificou-se que: (i à medida que as raízes se afastam do microaspersor, a extração de água pelas raízes do limoeiro é reduzida; (ii o limoeiro extrai mais água do solo quando não há restrição de disponibilidade hídrica; (iii sempre que os intervalos entre as irrigações aumentam, cresce também a área de extração de água pelo sistema radicular do limoeiro; (iv as raízes das plantas com diâmetros entre 0,5-2 mm foram as que mais extraíram água do solo.The present work had the objective of characterizing water uptake by 'Taiti' lemon (Citrus latifolia Tanaka roots in the soil profile under different microsprinkler irrigation intervals. The work was conducted under semi-arid conditions, in a sandy loam soil. Water uptake by roots was determined from soil water content by means of TDR probes, at several positions in the soil profile between two irrigation events. The root distribution was evaluated based upon data of length and root diameter at the same locations of TDR probes. According to the results, it was verified that: (i water uptake reduced as the distance root-microsprinkler increased; (ii lemon crop extracts more water when there is no restriction to the available water in the wetted volume; (iii the area of water uptake by lemon roots increases with increase in the irrigation intervals; (iv roots with

  11. Decomposition dynamics of mixed litter in a seasonally flooded forest near the Orinoco river

    Science.gov (United States)

    Bastianoni, Alessia; Chacón, Noemí; Méndez, Carlos L.; Flores, Saúl

    2015-04-01

    We evaluated the decomposition of a litter mixture in the seasonally flooded forest of a tributary of the Orinoco river. This mixture was prepared using three litter species, based on the litter fall rate observed over a complete hydro-period (2012-2013). The mixture loading ratio was 0.46 of Pouteria orinocoensis (Sapotaceae), 0.38 of Alibertia latifolia (Rubiaceae) and 0.16 of Acosmium nitens (Fabaceae). The initial chemical composition of each single litter species was also determined. Litterbags (20 × 20 cm, 2 mm opening) containing either each single species or the mixture, were deployed on the flooded forest soil and sampled after 30, 240, 270, 300 and 330 days. There were differences in initial total N and P concentrations, with A. nitens (AN) showing the highest nutrient concentrations (%NAN = 1.86 ± 0.19; %PAN = 0.058 ± 0.008) and P. orinocoensis (PO) and A. latifolia (AL) the lowest (%NPO = 0.92 ± 0.06; %NAL = 1.04 ± 0.04; %PPO = 0.029 ± 0.005; %PAL = 0.032 ± 0.001). Litter from AN showed the greatest mass loss (55%) and fastest decomposition rate (k = 0.00185 ± 0.00028) while litter from AL and the mixture showed the smallest mass loss (24% and 27% respectively) and the slowest decomposition rate (kAL = 0.00078 ± 0.00012 and kMIX = 0.00077 ± 0.00006). Decomposition rates were significantly and positively correlated with initial N (r = 0.556, p < 0.05) and P concentrations (r = 0.482, p < 0.05). Nevertheless, there were no significant differences between the expected decomposition rate and the observed decomposition rate of the mixture (additive response). To test the nature of the additivity, an enhancement factor (f) on decomposition rates for each single species was calculated. The species with the highest and smallest value of f were AN and AL, respectively. The fact that two out of the three species had values significantly different from 1, suggests that the additivity detected in our mixture was a consequence of the counterbalancing of

  12. SEX-DETector: A Probabilistic Approach to Study Sex Chromosomes in Non-Model Organisms

    Science.gov (United States)

    Muyle, Aline; Käfer, Jos; Zemp, Niklaus; Mousset, Sylvain; Picard, Franck; Marais, Gabriel AB

    2016-01-01

    We propose a probabilistic framework to infer autosomal and sex-linked genes from RNA-seq data of a cross for any sex chromosome type (XY, ZW, and UV). Sex chromosomes (especially the non-recombining and repeat-dense Y, W, U, and V) are notoriously difficult to sequence. Strategies have been developed to obtain partially assembled sex chromosome sequences. Most of them remain difficult to apply to numerous non-model organisms, either because they require a reference genome, or because they are designed for evolutionarily old systems. Sequencing a cross (parents and progeny) by RNA-seq to study the segregation of alleles and infer sex-linked genes is a cost-efficient strategy, which also provides expression level estimates. However, the lack of a proper statistical framework has limited a broader application of this approach. Tests on empirical Silene data show that our method identifies 20–35% more sex-linked genes than existing pipelines, while making reliable inferences for downstream analyses. Approximately 12 individuals are needed for optimal results based on simulations. For species with an unknown sex-determination system, the method can assess the presence and type (XY vs. ZW) of sex chromosomes through a model comparison strategy. The method is particularly well optimized for sex chromosomes of young or intermediate age, which are expected in thousands of yet unstudied lineages. Any organisms, including non-model ones for which nothing is known a priori, that can be bred in the lab, are suitable for our method. SEX-DETector and its implementation in a Galaxy workflow are made freely available. PMID:27492231

  13. Land Contamination and Soil-Plant Interactions in the Imperina Valley Mine (Belluno, Venetian Region, Italy)

    Science.gov (United States)

    Bini, Claudio; Wahsha, Mohammad; Fontana, Silvia; Zilioli, Diana

    2010-05-01

    In Italy, ore exploitation, particularly that of mixed sulphides, has been abandoned since the final thirty years of the last century, and a quantity of mine dumps has been discharged in wide areas of the land, provoking evident environmental damages to landscape, soil and vegetation, with potential risk for human health. The present study concerns the distribution and mobility of heavy metals (Ni, Cr, Cu, Pb, Zn, Fe and Mn) in the soils of a mine site and their transfer to wild flora. Soils and wild plants were sampled from mixed sulphides mine dumps in Imperina valley (Belluno, Italy), and the concentrations of heavy metals were determined. Chemical analyses carried out on 10 soil profiles (mostly entisols) of the mineralised area revealed metal concentrations generally above the international target levels (Cu up to 3160 mg kg-1 , Pb up to 23600 mg kg-1, Zn up to 1588 mg kg-1, Fe up to 52,30 %). The concentrations of Ni, Cr and Mn, instead, are below the reference limits. Moreover, a highly significant correlation was observed between the concentrations of metals in soils (Fe, Pb, Zn and Cu). Metal concentration in selected wild plants of the mineralized area is moderately high, in particolar Cu, Pb, Zn in the roots of Plantago major, Pb and Zn in the leaves of Taraxacum officinale, Zn and Pb in Salix spp. The translocation coefficient (BAC) from soil to plant (hypogean portion), and within the plant (epigean portion) vary from 0,37 in Plantago major to 2,97 in Silene dioica, two known accumulator plants. Salix spp present high translocation coefficients from soil to plant, and from roots to leaves. In particular, essential metals present a translocation coefficient ≥1 (with the order Mn>Zn>Cu>Fe), while toxic metals have coefficients Plantago major has very high metal concentrations in the roots and could be recommended for phytostabilization.

  14. Development of efficient RNA interference system using EGF-displaying phagemid particles

    Institute of Scientific and Technical Information of China (English)

    Hua JIANG; Xiu-mei CAI; Bi-zhi SHI; Jie ZHANG; Zong-hai LI; Jian-ren GU

    2008-01-01

    Aim:To develop an efficient RNA interference system using phagemid particles displaying the epidermal growth factor (EGF) ligand. Methods:pSilencer 1.0-siEGFP and pSilencer4.1-siAkt plasmids were constructed by gene clone technology. The modified helper phage genome (plasmid) M 13KO7EGFCT was used to pack-age phagemids, such as pSilencer 1.0-siEGFP and pSilencer4.1-siAkt. ELISA was used to quantify the titer of the progeny virus particles. Single-strand DNA was extracted and analyzed by agarose gel electrophoresis to evaluate the percentage of the phagemid particles. The expression level of the reporter gene enhanced green fluorescence protein (EGFP) was determined by transducing phagemid par-ticles packaging pSileneer 1.0-siEGFP into cells. The level of Akt gene expression in cells transduced phagemid particles packaging pSilencer4. 1-siAkt was exam-ined by Western blotting. Hydroxycamptothecin (HCPT) was used to enhance the gene transduction efficiency. Results:RNAi vectors pSilencer1.0-siEGFP and pSilencer4.1-siAkt were successfully constructed. Phagemid-encoding siRNA can be packaged efficiently. After the cells were infected by EGF displaying phagemid particles in the presence of HCPT, the expression of the target gene EGFPor Akt was substantially downregulated. Conclusion:Cell-targeted phagemid particles are efficient siRNA delivery vectors in the presence of HCPT.

  15. Large entropic effects on the thermochemistry of silicon nanodusty plasma constituents.

    Science.gov (United States)

    Seal, Prasenjit; Truhlar, Donald G

    2014-02-19

    Determination of the thermodynamic properties of reactor constituents is the first step in designing control strategies for plasma-mediated deposition processes and is also a key fundamental issue in physical chemistry. In this work, a recently proposed multistructural statistical thermodynamic method is used to show the importance of multiple structures and torsional anharmonicity in determining the thermodynamic properties of silicon hydride clusters, which are important both in plasmas and in thermally driven systems. It includes five different categories of silicon hydride clusters and radicals, including silanes, silyl radicals, and silenes. We employed a statistical mechanical approach, namely the recently developed multistructural (MS) anharmonicity method, in combination with density functional theory to calculate the partition functions, which in turn are used to estimate thermodynamic quantities, namely Gibbs free energy, enthalpy, entropy, and heat capacity, for all of the systems considered. The calculations are performed using all of the conformational structures of each molecule or radical by employing the multistructural quasiharmonic approximation (MS-QH) and also by including torsional potential anharmonicity (MS-T). For those cases where group additivity (GA) results are available, the thermodynamic quantities obtained from our MS-T calculations differ considerably due to the fact that the GA method is based on single-structure data for isomers of each stoichiometry, and hence lack multistructural effects; whereas we find that multistructural effects are very important in silicon hydride systems. Our results also indicate that the entropic effect on the thermochemistry is huge and is dominated by multistructural effects. The entropic effect of multiple structures is also expected to be important for other kinds of chain molecules, and its effect on nucleation kinetics is expected to be large.

  16. SEX-DETector: A Probabilistic Approach to Study Sex Chromosomes in Non-Model Organisms.

    Science.gov (United States)

    Muyle, Aline; Käfer, Jos; Zemp, Niklaus; Mousset, Sylvain; Picard, Franck; Marais, Gabriel Ab

    2016-01-01

    We propose a probabilistic framework to infer autosomal and sex-linked genes from RNA-seq data of a cross for any sex chromosome type (XY, ZW, and UV). Sex chromosomes (especially the non-recombining and repeat-dense Y, W, U, and V) are notoriously difficult to sequence. Strategies have been developed to obtain partially assembled sex chromosome sequences. Most of them remain difficult to apply to numerous non-model organisms, either because they require a reference genome, or because they are designed for evolutionarily old systems. Sequencing a cross (parents and progeny) by RNA-seq to study the segregation of alleles and infer sex-linked genes is a cost-efficient strategy, which also provides expression level estimates. However, the lack of a proper statistical framework has limited a broader application of this approach. Tests on empirical Silene data show that our method identifies 20-35% more sex-linked genes than existing pipelines, while making reliable inferences for downstream analyses. Approximately 12 individuals are needed for optimal results based on simulations. For species with an unknown sex-determination system, the method can assess the presence and type (XY vs. ZW) of sex chromosomes through a model comparison strategy. The method is particularly well optimized for sex chromosomes of young or intermediate age, which are expected in thousands of yet unstudied lineages. Any organisms, including non-model ones for which nothing is known a priori, that can be bred in the lab, are suitable for our method. SEX-DETector and its implementation in a Galaxy workflow are made freely available. PMID:27492231

  17. Epigenetic changes of Arabidopsis genome associated with altered DNA methyltransferase and demethylase expressions after gamma irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Ji Eun; Cho, Eun Ju; Kim, Ji Hong; Chung, Byung Yeoup; Kim, Jin Hong [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2012-05-15

    DNA methylation at carbon 5 of cytosines is a hall mark of epigenetic inactivation and heterochromatin in both plants and mammals. In Arabidopsis, DNA methylation has two roles that protect the genome from selfish DNA elements and regulate gene expression. Plant genome has three types of DNA methyltransferase, METHYLTRANSFERASE 1 (MET1), DOMAINREARRANGED METHYLASE (DRM) and CHROMOMETHYLASE 3 (CMT3) that are capable of methylating CG, CHG (where H is A, T, or C) and CHH sites, respectively. MET1 is a maintenance DNA methyltransferase that controls CG methylation. Two members of the DRM family, DRM1 and DRM2, are responsible for de novo methylation of CG, CHG, and CHH sites but show a preference for CHH sites. Finally, CMT3 principally carries out CHG methylation and is involved in both de novo methylation and maintenance. Alternatively, active DNA demethylation may occur through the glycosylase activity by removing the methylcytosines from DNA. It may have essential roles in preventing transcriptional silencing of transgenes and endogenous genes and in activating the expression of imprinted genes. DNA demetylation in Arabidopsis is mediated by the DEMETER (DME) family of bifunctional DNA glycosylase. Three targets of DME are MEA (MEDEA), FWA (FLOWERING WAGENINGEN), and FIS2 (FERTILIZATION INDEPENDENT SEED 2). The DME family contains DEMETER-LIKE 2 (DML2), DML3, and REPRESSOR OF SILENING 1 (ROS1). DNA demetylation by ROS1, DML2, and DML3 protect the hypermethylation of specific genome loci. ROS1 is necessary to suppress the promoter methylation and the silencing of endogenous genes. In contrast, the function of DML2 and DML3 has not been reported. Several recent studies have suggested that epigenetic alterations such as change in DNA methylation and histone modification should be caused in plant genomes upon exposure to ionizing radiation. However, there is a lack of data exploring the underlying mechanisms. Therefore, the present study aims to characterize and

  18. Land contamination and soil evolution in abandoned mine areas (Italy)

    Science.gov (United States)

    Bini, Claudio; Wahsha, Mohammad; Spiandorello, Massimo

    2014-05-01

    In Italy ore research and exploitation are nearly exhausted since the end of the last century, leaving on the land a huge amount of mine waste, therefore provoking evident environmental damage including landscape, vegetation and the food chain, and a potential threat to human health. The increasing environmental consciousness of general population compelled Public Administrators to set down effective legislation acts on this subject (e.g. D.L. 152/2006), and more generally on environmental contamination. In this work we present the results of a survey carried out at several mixed sulphides mine sites in Italy, exploited for at least a millennium, and closed in the '60s of the last century. Biogeochemical analyses carried out on 50 soil profiles (mostly Entisols and Inceptisols) and vegetation in the proximal and distal areas of ore exploitation show metal concentrations overcoming legislation limits on average (Cu up to 3160 mg kg-1 , Pb up to 23600 mg kg-1, Zn up to 1588 mg kg-1, Fe up to 52,30 %). Ni, Cr and Mn concentrations, instead, are generally below the reference levels. Metal concentrations in native vegetation of the examined areas are moderately to highly elevated. Significant amounts of Cu, Pb, Zn in roots of Plantago major and Silene dioica, in leaves of Taraxacum officinale, and Salix spp, have been recorded. Essential elements, in particular, present Translocation Coefficients (TC) >1, with Mn>Zn>Cu>Fe. Toxic elements (Cd, Cr, Pb), instead, present TCparks in these areas could enhance their educational and scientific value, contributing in the meantime to general population amusement and recreation. Finally, it is the occasion for soil scientists to submit to the scientific community new classification proposals of this new kind of soils. Key-words: mine waste, heavy metals, accumulator plants, phytoremediation, soil genesis, soil classification

  19. Determination of lead in papers and cardboards for food packaging; La determinazione del piombo in carte e cartoni per alimenti. Giornata di studio

    Energy Technology Data Exchange (ETDEWEB)

    Costantini, S.; Giordano, R. [eds.] [Istituto Superiore di Sanita`, Rome (Italy). Lab. di Tossicologia Applicata

    1998-06-01

    The meaning of a legal limit of lead in papers for food packaging is based on the fact that a material having high amount of lead could potentially contain other contaminants. The sample preparation represents a very important step that must be carefully considered in order to have a correct final result. The atomic absorption graphite furnace resulted the most utilized technique, since it offers both good accuracy and sensitivity; nevertheless, numerous factors influencing the determination must be considered. Besides obtaining accurate values, the problem is to take correct decision in presence of a variability not eliminable; in this case, the statistics can give a valid help by means of a mathematical model. The suitability of the materials used in a Quality control programme must be considered a parameter of capital importance, since the performance of the laboratories could be heavily influenced by the quality of samples. During 1997, a collaborative trial has been carried out with the participation of 70 laboratories of the national service. [Italiano] Il significato della presenza di un limite di piombo nelle carte destinate al contatto con alimenti risiede nel fatto che un materiale che non rispetta tale parametro puo` contenere altri contaminanti non prevedibili. La preparazione del campione da sottoporre alla determinazione e` una fase molto delicata da non sottovalutare ai fini della correttezza del risultato analitico finale. La tecnica dell`assorbimento atomico a fornace di grafite e` risultata la piu` utlizzata, in funzione delle buone accuratezza e sensibilita` del metodo; tuttavia, numerosi fattori che possono influenzare negativamente questo tipo di analisi devono essere tenuti in considerazione. Datala presenza di un limite di legge, il problema non e` solo quello di ottenere valori precisi ed accurati, ma di prendere decisioni corrette in presenza di una variabilita` di fondo non eliminabile. La statistica decisionale, attraverso un modello

  20. Anthropogenically derived mercury in sediments of Pialassa Baiona, Ravenna, Italy

    Energy Technology Data Exchange (ETDEWEB)

    Fabbri, D.; Gemelli, S.; Vassura, I. [Bologna Univ., Bologna (Italy). Lab. di Chimica Ambientale; Langone, L.; Miserocchi, S. [Consiglio Nazionale delle Ricerche, Istituto di Geologia Marina, Bologna (Italy); Trombini, C. [Bologna Univ., Bologna (Italy). Dipt. di Chimica G. Ciamician

    2001-10-01

    Pialassa Baiona, a coastal wetland near the city of Ravenna, has been impacted for two decades (1958-1978) by industrial discharges containing mercury and floating agglomerates of residues of polymerization processes. Although the industrial use of mercury completely ceased in the early 90's, surface sediments are still highly contaminated, mercury concentrations decreasing from the southern sub-basin, close to the discharge point, to the farthest northern border. Concentrations of total mercury, synthetic polymers (determined by pyrolysis-GC/MS), total organic carbon, C/N ratio, total sulfur and {sup 210}Pb dating, were determined in sediment cores collected in the southern and northern sub-basins. Mercury and polymers exhibited parallel profiles with a peak corresponding to the historic emission record in the southern core, while in northern cores peaks of maximum concentration display a delay reflecting the time required for the pollutants to migrate. A recently developed mercury sequential extraction procedure was applied to the most polluted layers to study inorganic mercury speciation. Results indicate differences between the southern and northern areas, suggesting a more efficient binding of mercury to sediments in the southern sub-basin. [Italian] La Pialassa Baiona, una laguna costiera in prossimita' della citta' di Ravenna, per due decadi (1958-1978) e' stata sottoposta all'impatto di scarichi industriali contenenti, fra l'altro, mercurio ed agglomerati gallegianti di residui di processi di polimerizzazione. Sebbene l'uso industriale di mercurio sia completamente cessato all'inizio degli anni 90, i sedimenti superficiali sono tuttora fortemente contaminati, con concentrazioni che decrescono muovendosi dal bacino meridionale che riceveva gli scarichi, in direzione del confine settentrionale. In carote di sedimento campionate nei sotto-bacini meridionale e settentrionale sono state determinate le concentrazioni di

  1. Introduction of a new management programme for the urban solid waste in the placed organized by 'Consorzio AREA' of Copparo (FE). Verification at pilot scale; Introduzione di un nuovo sistema per la gestione dei rifiuti solidi urbani nel territorio del Consorzio AREA di Copparo (FE). Verifica pilota del sistema

    Energy Technology Data Exchange (ETDEWEB)

    Ceccotto, D.; Barboni, A.; Malago, L.; Sunseri, M.; Spiandorello, R. [Consorzio Aziendale di Igiene Ambientale e Servizi Polivalenti, Copparo, FE (Italy)

    1999-08-01

    , minimizzare i costi dell'esercizioi dello stesso, sono state verificate tremite l'applicazione del sistema ad un campione pilota, rappresentativo dell'intero bacino. E' inoltre presentatao in allegato lo studio per l'ottimazione dei percorsi di raccolta tramite metodologie numeriche computerizzata.

  2. ELABORAZIONE DI UN QUESTIONARIO PER LA RILEVAZIONE DEI BISOGNI COMUNICATIVI DEGLI ADULTI IMMIGRATI

    Directory of Open Access Journals (Sweden)

    Annamaria Aquilino

    2012-02-01

    Full Text Available L’intensità del fenomeno migratorio in Italia suscita una riflessione critica sui bisogni linguistici di un  nuovo pubblico di apprendenti, spinti verso la conoscenza della lingua italiana da una forte esigenza di integrazione sociale. L’articolo presenta l’elaborazione di un questionario come strumento d’indagine essenziale per la rilevazione dei bisogni comunicativi degli immigrati adulti. Progettato nell’ambito di un corso di italiano L2  presso il CTP di Rozzano (Milano e sottoposto a un campione di 24 stranieri, il questionario si  è rivelato molto utile per la definizione del profilo dei singoli apprendenti e dell’intera classe che, caratterizzata da una grande differenziazione, non è sempre facile da gestire. L’interpretazione finale dei dati ha messo in luce non solo gli elementi di diversità ma ha anche permesso di cogliere alcuni tratti omogenei molto interessanti, indispensabili per la programmazione del percorso didattico, che sarà tanto efficace quanto più si adatterà alla realtà psicologica e socio-culturale degli apprendenti.     Questionnaire design to Survey the communicative needs of adult immigrants   Widespread immigration in Italy has brought about critical reflection on the linguistic needs of a new group of learners, highly motivated to learn the Italian language because of their strong need for social integration.  This article presents the designing of a questionnaire as an essential tool for surveying the communicative needs of adult immigrants.  Designed for an Italian L2 course held at the CTP in Rozzano (Milano and administered to 24 foreigners, the questionnaire was very useful for defining the profile of the single learners and the whole class group, which was dissimilar, and thus not the easiest to conduct.  The final interpretation of the data brought to light not only the elements of diversity but they also allowed us to recognize a few interesting common traits, essential for

  3. Indagini preliminari su una collezione osteologica di orso delle caverne (Ursus spelaeus Rosenmüller & Heinroth provenienti da una grotta in val di Lima (Toscana

    Directory of Open Access Journals (Sweden)

    G. Bresci

    2003-10-01

    morfologia dentaria che ha permesso, anche se con precisione approssimata visto il piccolo campione disponibile, di stimare una datazione del materiale raccolto.

  4. L’UMORISMO NELLA DIDATTICA DELL’ITALIANO PER STRANIERI. PROPOSTE DIDATTICHE .

    Directory of Open Access Journals (Sweden)

    Elisa Gironzetti

    2010-09-01

    Full Text Available L'articolo, dopo aver presentato le più avanzate ricerche di carattere pragmalinguistico che hanno contribuito a definire la "competenza umoristica" come una specifica abilità linguistico-comunicativa,  invita a considerare l'umorismo come uno strumento valido ed efficace per rendere la lezione di italiano per stranieri un momento di apprendimento, ma anche di socializzazione, di comprensione e tolleranza interculturale. I testi umoristici costituiscono infatti un campione di lingua autentico, ricco di contenuti linguistici, paralinguistici e culturali, insomma, un ottimo spunto per la creazione di attività didattiche non solo interessanti, ma anche divertenti e motivanti. L'obiettivo finale di questo studio è proporre attività didattiche - di cui si fornisce esempi -  per insegnare (con l'umorismo nel contesto di una classe di lingua italiana per stranieri ispanofoni. Le attività didattiche proposte si basano sull'analisi di un corpus bilingue spagnolo/italiano di testi umoristici prestando particolare attenzione agli elementi linguistici e paralinguistici che caratterizzano l'umorismo nelle due lingue e ai contenuti socioculturali specifici necessari per poter comprendere il testo umoristico. Abstract   After a thorough survey of the latest research regarding pragmatic-linguistic issues which have contributed to defining "humor competence" as a specific linguistic-communicative skill, this article suggests considering humor as a valid and effective tool to make Italian language lessons for foreigners into moments for learning as well as socialization, understanding and intercultural tolerance. Humorous texts present authentic language, rich with linguistic, paralinguistic and cultural content, and they are an excellent stimulus for creating interesting, fun and motivating didactic activities. The aim of this study is to propose didactic activities - with examples- to teach humor (with humor in the context of an Italian language class

  5. La qualitá percepita dei pazienti con infezione da HIV presso il servizio di diagnosi e cura dell' AIDS di Novara

    Directory of Open Access Journals (Sweden)

    M. Renna

    2003-05-01

    Full Text Available

    Obiettivi: costruire e sperimentare un modello per lo studio della qualità percepita dai pazienti affetti da HIV/AIDS.

    Metodi: è stato condotto uno studio di tipo osservazionale descrittivo mediante un’indagine con l’utilizzo di un questionario di soddisfazione presso il Servizio di diagnosi e terapia dell’AIDS dell’UOA di Malattie Infettive dell’ASO “Maggiore della Carità” di Novara. I dati sono stati analizzati mediante l’analisi fattoriale e l’analisi dei cluster.

    Risultati: il campione è stato rappresentato da 62 soggetti (35 uomini e 27 donne, età media di 39,40 anni. Sono stati identificati tre fattori principali determinanti la soddisfazione dei pazienti: l’assistenza infermieristica (che ha spiegato il 24,03% della variabilità delle risposte, l’assistenza medica (21,18% e l’organizzazione del servizio (22,22%. Nel dettaglio livelli di soddisfazione elevati sono stati registrati per la qualità dell’assistenza del personale, la competenza dei medici ed il funzionamento della terapia, mentre i più bassi sono stati misurati per il rispetto della privacy e la disponibilità di servizi accessori. L’analisi dei cluster ha permesso di aggregare gli utenti in 3 gruppi in base al livello di soddisfazione. I fattori che hanno maggiormente determinato il diverso livello di soddisfazione misurato nei tre gruppi sono stati: la prima accoglienza presso il servizio, il livello di informazione ricevuto e l’efficacia nel controllo del dolore e dei sintomi.

    Conclusioni: il modello di analisi adottato ha reso possibile l’identificazione dei determinanti della soddisfazione degli utenti afferenti al servizio, evidenziando gli elementi di criticità del percorso assistenziale. I risultati hanno dimostrato la necessità per gli studi di valutazione della qualità percepita di adottare i metodi e gli strumenti della ricerca

  6. Valutazione della qualitá dei dati in uno studio post marketing

    Directory of Open Access Journals (Sweden)

    D. D’Alessandro

    2003-05-01

    fornito un dato che si discosta da quello atteso, comportando un'imprecisione nella stima del rischio per i 928 anziani da loro monitorati, pari al 3,2% dell'intero campione.

  7. Polimorfismi nei geni metabolici come fattori dirischio per il carcinoma gastrico (GC: risultati preliminari di uno studio caso-controllo

    Directory of Open Access Journals (Sweden)

    S. Boccia

    2003-05-01

    Full Text Available

    Obiettivi: valutare le frequenze dei principali geni metabolici in un campione di casi di GC (Carcinoma Gastrico e di controlli, per determinare se esiste un’associazione con il rischio di GC, e considerare le possibili associazioni tra i genotipi studiati ed alcuni parametri clinico-patologici.

    Metodi: è stato condotto uno studio caso-controllo ospedaliero, tra novembre 1999 e maggio 2003 reclutando 48 pazienti con diagnosi di adenocarcinoma gastrico sottoposti a gastrectomia, e 48 controlli appaiati per sesso ed età presso il Policlinico Universitario “A. Gemelli” in Roma. Per genotipizzare gli individui rispetto ai geni GSTM1, NAT2, SULT1A1, CYP1A1, CYP2E1 (5’ flanking e introne 6, è stata usata la tecnica della PCR con analisi del profilo dei frammenti di restrizione (PCR-RFLP. L’analisi statistica ha previsto l’impiego di test non parametrici e della regressione logistica.

    Risultati: le frequenze dei polimorfismi nei geni metabolici sono risultate in alcuni casi differenti nei casi rispetto ai controlli sebbene non si evidenzi una differenza statisticamente significativa. In particolare il genotipo GSMT1 null è più elevato nei pazienti rispetto ai controlli (63.8% vs. 51% e gli individui NAT2 acetilatori lenti risultano più frequenti nei casi rispetto ai controlli (69.6% vs. 57.45%. L’istotipo diffuso di GC è associato significativamente ai pazienti eterozigoti per l’allele SULT1A1 (c_= 8.216; p= 0.018, ed i pazienti NAT2 acetilatori lenti tendono ad avere un grading tumorale alto (c_= 7.425; p= 0.006.

    Conclusioni: i risultati preliminari di questo studio evidenziano associazioni tra alcuni genotipi metabolici e parametri clinico-patologici, che se confermati potrebbero permettere di identificare sottogruppi con prognosi più sfavorevole di GC da indirizzare preventivamente verso trattamenti specifici.

  8. L’impatto del ricovero ospedaliero sulla qualità della vita dei pazienti: uno studio prospettico

    Directory of Open Access Journals (Sweden)

    Luca Giuseppe Re

    2012-05-01

    Full Text Available Background. Secondo l’Organizzazione Mondiale della Sanità la qualità di vita (QdV è “la personale percezione che un individuo ha della propria posizione nella vita, nel contesto di una cultura e di un insieme di valori nei quali egli vive, anche in relazione ai propri obiettivi, aspettative e preoccupazioni”. Numerosi sono gli strumenti psicometrici progettati per misurare in ambito sanitario l’impatto dell’erogazione di interventi clinico-assistenziali sulla QdV degli utenti, tuttavia poca attenzione si è posta sul ruolo di alcuni predittori - socio-demografici, clinici ed economico-sanitari - durante la degenza ospedaliera. Obiettivi. Accertare l’esistenza ed il tipo di associazione tra alcuni predittori e la variazione della QdV alla dimissione; esaminare i possibili risvolti per la pratica infermieristica. Metodi. Lo studio, condotto presso la struttura ospedaliera del polo universitario di Milano, ha somministrato all’ingresso e alla dimissione dei pazienti lo strumento psicometrico EuroQol-5D. Per valutare l’impatto del trattamento sulla QdV è utilizzato il test ?2, mentre per misurare significatività e rilevanza dei predittori è stato creato un modello di regressione logistica multipla. Risultati. L’erogazione di un trattamento clinico durante il ricovero migliora la QdV percepita (p < 0.001. La proporzione di esiti positivi nei trattamenti medici rispetto a quelli chirurgici è superiore (p < 0.05, così come quella nei ricoveri urgenti (p < 0.01. Il modello di regressione logistica multipla mostra associazioni significative tra età (p < 0.05, degenza (p < 0.05, tempo di intervento (p < 0.01 e variazione della QdV. Limiti. Studio monocentrico; campione di convenienza; periodo di osservazione limitato con possibili scostamenti nel numero e tipologia di ricoveri rispetto alla media annua. Conclusioni. La QdV percepita alla dimissione migliora in caso di erogazione di un trattamento clinico, in particolare se

  9. Habitat use by the european wild rabbit (Oryctolagus cuniculus in a coastal sandy dune ecosystem of central italy

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    Francesco Santilli

    2010-08-01

    Full Text Available Abstract
    The European wild rabbit (Oryctolagus cuniculus has been recurrently introduced into Italy since the Roman times. Some populations occur along sandy coastal dunes (Central Italy. There is no information about the ecology and habitat preferences of wild rabbits in this particular ecosystem. During May and June 2009, we assessed the distribution of wild rabbits in a 120 ha protected area (province of Livorno, Tuscany, by faecal pellet counts in 170 circular plots (d = 1 m. Range size defined by Kernel Analysis was 27.3 ha (95% and 13.2 ha (50%. Habitat selection was assessed through Jacobs’ index of selection. Rabbits selected the first dunes that offer both food (psammophilous grasses and the cover of tamarisk shrubs. Holm oak woods and pinewoods were avoided, probably because of the lack of undergrowth.

    Riassunto
    Uso dell'habitat da parte del coniglio selvatico (Oryctolagus cuniculus in un ecosistema dunale costiero dell’Italia centrale. In Italia sono presenti diverse popolazioni di coniglio selvatico (Oryctolagus cuniculus derivanti da introduzioni effettuate in epoche diverse. Alcune di queste sono localizzate lungo le coste sabbiose della Toscana. Le informazioni sull’ecologia e sulle preferenze ambientali delle popolazioni che vivono in questi particolari ecosistemi sono molto carenti. In questo studio è stato analizzato l’uso dell'habitat da parte del coniglio selvatico in un area costiera protetta della provincia di Livorno delle dimensioni di 120 ha. La distribuzione di questo lagomorfo è stata stimata nel periodo maggio - giugno 2009 in base alla presenza/assenza delle feci in 170 cerchi campione di 1 m di diametro. L’areale occupato dalla specie è stato definito tramite Kernel Analysis al 95% (27.3 ha e 50% (13.2 ha. La selezione di habitat è stata stimata tramite l’indice di Jacobs. Il coniglio selvatico

  10. The diet of the fox (Vulpes vulpes in woodlands of Orobie Alps (Lombardy region, Northern Italy / Alimentazione della Volpe (Vulpes vulpes in aree boscate delle Alpi Orobie

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    Marco Cantini

    1991-07-01

    Full Text Available Abstract The diet of the fox was investigated by analysis of 273 scats, collected along standard trails from April to November 1987 and 1988. Food habits of foxes were described for three altitudinal ranges. Mammals, mainly Clethrionomys glareolus and Microtus multiplex, were the staple food (percentage of frequency 42.8%, followed by fruits and other vegetables (26.7% and 37.3% respectively. Birds, Invertebrates (mainly Insects and garbage were little eaten. The game species (ungulates, hares, pheasants occurred with a low frequency (8.4% in the diet. The trophic niche breadth varied little through the altitudinal ranges and the seasons. The trophic niche overlap between the fox and the genus Martes (190 scats of M. martes and M. foina were examined is relatively wide (O=0.868. Riassunto La dieta della Volpe (Vulpes vulpes in aree boscate delle Alpi Orobie (Val Lesina è stata indagata nel periodo aprile-novembre 1987 e 1988 mediante l'analisi di 273 feci, raccolte lungo percorsi-campione ricadenti in tre piani vegetazionali. I Mammiferi, in particolare Clethrionomys glareolus e Microtus multiplex, sono la componente principale della dieta (frequenza percentuale 42,8%. Rilevante è anche il consumo di frutti (soprattutto in estate e autunno e di altri vegetali (26,7% e 37,3% rispettivamente, mentre poco frequente è quello di Uccelli, Invertebrati e rifiuti. Complessivamente ridotta è l'azione predatoria della Volpe nei confronti delle specie di interesse venatorio (Ungulati, lepri, Galliformi. L'ampiezza della nicchia trofica mostra modeste variazioni stagionali e altitudinali. I1 grado di sovrapposizione tra la nicchia trofica della Volpe e quella del genere Martes, quest'ultima ricavata dall'analisi di 190 feci di Martora (M. martes e Faina (M. foina, è elevato (O=0,868. Tuttavia, poiché in condizioni di

  11. La promozione del parto naturale: effetti di un ambulatorio dedicato sulla prevalenza del taglio cesareo

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    Valentina Simonetti

    2015-04-01

    Full Text Available Background. Il taglio cesareo (TC è una procedura chirurgica eseguita in tutto il mondo, spesso senza una reale necessità, con un trend in continuo aumento sopratutto nei paesi industrializzati. In Italia la prevalenza del TC è più elevata rispetto ad altri paesi europei. Tale pratica espone le donne a rischio di complicanze evitabili e aumenta i costi dell’assistenza sanitaria. Tra i fattori riconducibili al ricorso al TC vengono riportate: carenze organizzative, medicina difensiva, convinzione che sia una modalità elettiva di nascita, scarso supporto alla donna del personale sanitario nel percorso di accompagnamento alla nascita. Diverse evidenze suggeriscono che interventi educativi rivolti alle donne in gravidanza aumentano la probabilità del parto naturale (PN rispetto al cesareo: un ambulatorio dedicato al PN potrebbe risultare determinante nel dipanare dubbi ed incertezze della gestante e promuovere la scelta del parto naturale rispetto al TC. Obiettivi. Confrontare la prevalenza dei TC prima e dopo l’implementazione di un ambulatorio dedicato alla promozione del PN. Metodi. L’indagine è stata condotta consultando i dati delle cartelle cliniche delle pazienti afferenti al Dipartimento Materno-Infantile della Azienda USL di Pescara dal 1 febbraio al 31 ottobre 2011, prima dell’attivazione dell’ambulatorio, e dal 1 febbraio al 31 ottobre 2012, dopo l’attivazione dello stesso. Sono state incluse tutte le schede ostetriche delle donne con gravidanza a termine (37 settimane di gestazione; feto vivo in presentazione cefalica; precedente parto spontaneo; precedente TC eseguito in travaglio con buona dilatazione cervicale raggiunta; intervallo tra i parti di 18-24 mesi. Risultati. Sul totale delle cartelle esaminate (2.623, il campione per l’anno 2011 e 2012 è costituito rispettivamente da donne che hanno espletato prevalentemente il PN (62%; 68% con un’età media di 32.0±1.9 e 31.6±0.3, di nazionalità italiana (82%; 87

  12. Misure di tenacità a frattura su acciai utilizzando velocità di deformazione elevate

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    Enrico Lucon

    2007-10-01

    Full Text Available La conoscenza delle proprietà meccaniche di tipo dinamico per i materiali metallici è utile ogniqualvolta la sensibilità alla velocità di deformazione è di rilevanza per un acciaio, e qualora le condizionireali di carico per una struttura (in caso di normale esercizio o di situazioni d'emergenza siano diverse dal caso statico. Inoltre, in alcuni studi l'aumento della velocità di deformazione serve a simulare gli effetti di altri meccanismi di fragilizzazione quali l'invecchiamento termico (thermal ageing o l'irraggiamento.La presente memoria fornisce una panoramica dell'esperienza maturata al Centro Nucleare Belga (SCK•CEN nel campo delle misure di tenacità a frattura su acciai in condizioni di velocità di deformazione elevata, con particolare riguardo alle prove di resilienza strumentata su provini Charpy precriccati (PCVN.Dopo una breve dissertazione sui meccanismi fondamentali che aiutano a comprendere gli effetti della velocità di deformazione sulla tenacità degli acciai in regime fragile e duttile, vengono presentate le procedure sperimentali ed analitiche per misurare la tenacità a frattura con velocità di deformazione elevata, prendendo in considerazione da un lato le principali normative internazionali (ASTM e ISO e dall'altro il lavoro di normazione attualmente in corso sotto il coordinamento di SCK•CEN: la revisione della norma ASTM E1921 (metodo della Master Curve per la misura della tenacità in regime di transizione duttile/fragile e lo sviluppo di una nuova norma ISO sulle prove di tenacità dinamica con provini PCVN. Quest'ultimo documento è presentato in maggior dettaglio, concentrando l'attenzione sulla determinazione della tenacità dinamica in campo fragile con il metodo dell'Impact Response Curve e in campo duttile (curve di esistenza J-R mediante l'uso di metodi mono- e multi-campione.In conclusione, vengono presentati alcuni esempi tratti dalla banca dati sviluppata da SCK•CEN per le misure di tenacit

  13. Stato nutrizionale dei bambini toscani di terza elementare

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    M. Giacchi

    2003-05-01

    Full Text Available

    La Regione Toscana nel 2001/2002 ha partecipato al progetto pilota nazionale “Sorveglianza ed educazione nutrizionale basata su dati locali per la prevenzione delle malattie cronico degenerative correlate all’obesità” al fine di sperimentare una metodologia per l’implemantazione di un sistema di sorveglianza nutrizionale in età pediatrica a livello regionale.

    Obiettivi: scopo di questo lavoro è approfondire le conoscenze sullo stato nutrizionale e descrivere la relazione che questo ha con il livello di istruzione materna e l’ampiezza demografica del comune di residenza.

    Metodi: il campione è composto da 3076 bambini di cui 1585 maschi e 1491 femmine di 8-9 anni. Lo stato nutrizionale è stato valutato tramite la rilevazione delle misure antropometriche, peso e altezza, ed il successivo calcolo dell’Indice di Massa Corporea (IMC; le classi di IMC sono state individuate secondo le indicazioni di Cole et al.; il livello di istruzione della madre è stato suddiviso in tre classi: basso, medio ed alto livello. Risultati: dall’analisi dei dati è emerso che la prevalenza del sovrappeso è del 22,6% (22,7% nei maschi e 22,5% nelle femmine e quella dell’obesità dell’8,8% (9,4% nei maschi e 8,2% nelle femmine. Il 58% dei bambini normopeso e il 43% degli obesi ha la madre con livello di istruzione alto, mentre il 4% dei normopeso e il 10,5% degli obesi ha la madre con livello di istruzione basso. La percentuale più bassa di obesità (6,5% è stata riscontrata in comuni con ampiezza demografica superiore ai 30000 abitanti.

    Conclusioni: la prevalenza del sovrappeso e dell’obesità in età pediatrica in Toscana è più bassa rispetto alla media nazionale. Esiste una relazione inversa tra l’Indice di Massa Corporea del bambino ed livello di istruzione della madre (p‹ .001.

  14. Caratterizzazione di ceppi Listeria monocytogenes isolati da formaggi a pasta molle e semi-molle prelevati nella regione Abruzzo

    Directory of Open Access Journals (Sweden)

    Vicdalia A. Acciari

    2011-01-01

    Full Text Available Sono stati caratterizzati 47 ceppi di Listeria monocytogenes isolati in un’indagine condotta su formaggi prelevati al dettaglio. Cinque formaggi (gorgonzola, taleggio, asiago, crescenza e brie sono stati selezionati tra quelli a pasta semi molle e molle di maggior consumo su scala nazionale e più frequentemente contaminati da Listeria monocytogenes. Per ogni ceppo è stato determinato il sierotipo, il pattern di resistenza agli antibiotici e il profilo di macrorestrizione con la Pulsed Field Gel Electrophoresis (PFGE. I principali sierotipi rilevati sono risultati l’1/2a (76,6% e l’1/2c (21,3% mentre il sierotipo 1/2b è stato rilevato solo in 1 campione. Il 97,9% dei ceppi è risultato resistente all’oxicillina, l’80,9% alla lincomicina e il 78,7% alla clindamicina. La resistenza a 2 antibiotici è stata rilevata nel 17% dei ceppi con due diversi pattern (OXCC, OXL mentre a 3 nel 70,2% con un unico profilo (OXCCL. Non sono stati rilevati ceppi sensibili a tutte le molecole testate. Con l’analisi combinata dei profili di macrorestrizione di AscI e ApaI, sono stati identificati 11 pulsotipi suddivisi in 3 cluster. Due pulsotipi sono risultati prevalenti raggruppando rispettivamente il 57,4% e il 21,3% dei ceppi isolati. L’analisi dei profili PFGE non ha rilevato relazioni tra pulsotipo e tipo di formaggio, ditta produttrice o punto vendita. La valutazione della distribuzione temporale dei pulsotipi ha messo in evidenza la presenza di un profilo persistente durante tutto il periodo di studio, ad eccezione dei mesi di agosto e settembre in cui è stato rilevato un pulsotipo diverso. Pertanto l’indagine ha permesso di rilevare la variabilità nel tempo della prevalenza di alcuni specifici pulsotipi che ha fatto ipotizzare l’intervento di fattori che hanno influenzato le dinamiche di contaminazione di questi prodotti. Studi su larga scala potrebbero contribuire al chiarimento di tale fenomeno.

  15. Urban traffic pollution and citizens information; Misure anti inquinamento da traffico urbano e partecipazione del cittadino. Uno studio di caso

    Energy Technology Data Exchange (ETDEWEB)

    Giuntarelli, P.; Borrelli, G.; Brini, S. [ENEA, Centro Ricerche Casaccia, Rome (Italy). Dipt. Ambiente

    1999-07-01

    su un campione di cittadini di due quartieri di Roma, si propone di esaminare il grado di accettazione sociale di una serie di misure tendenti a ridurre l'inquinamento atmosferico causato dal traffico veicolare urbano, dal momento che trova unanime consenso riconoscere al traffico veicolare e piu' in generale al sistema di vita urbano le maggiori responsabilita' per quanto riguarda la produzione di sostanze inquinanti dell'aria.

  16. Variabilità microsatellitare in popolazioni italiane di martora, Martes martes

    Directory of Open Access Journals (Sweden)

    Licia Colli

    2003-10-01

    Full Text Available La martora, Martes martes, è un mustelide storicamente presente su gran parte del territorio italiano, soprattutto dove siano presenti foreste mature altamente strutturate e ricche di risorse. Nel corso dei decenni passati, cause quali la deforestazione con conseguente frammentazione dell'habitat, la caccia e la persecuzione da parte dell'uomo hanno portato ad un sensibile declino sia nel numero d'individui sia di popolazioni in tutta l'Europa. Frammentazione degli areali e diminuzione del numero di soggetti costituiscono le cause principali di un limitato flusso genico con conseguente formazione di popolazioni isolate di martora e perdita di variabilità genetica (Kyle et al., 2003. Per queste ragioni la martora risulta attualmente tutelata secondo quanto previsto dalla Convenzione di Berna (appendice III e dalla Direttiva europea 92/43/CEE "Habitats & Species". Di fronte a prospettive di tipo conservazionistico, diventa necessario disporre di metodologie per la valutazione della variabilità residua nella specie a livello d'individui e di popolazioni. Secondo numerosi Autori, i microsatelliti costituiscono marcatori molto efficaci nella descrizione della struttura genetica e della storia demografica di specie (Beaumont & Bruford, 1999; Linares, 1999. Il presente lavoro propone i risultati ottenuti attraverso il confronto di due campioni costituiti da individui di Martes martes provenienti dalla Sardegna (25 esemplari e dal territorio italiano (6 esemplari. L'analisi è stata condotta a livello di 7 loci microsatellitari dinucleotidici polimorfici. Il valore di FST calcolato dal confronto dei due gruppi è risultato pari a 0,102 e ricade nel range di variazione identificato di recente in popolazioni nord-europee di martora (Kyle et al., 2003. L'analisi della varianza molecolare (AMOVA, condotta a due livelli gerarchici, ha permesso in particolare di ripartire la varianza del campione

  17. L’identificazione di virus citopatogeni isolati dall’ambiente:ricerca ed applicazione di protocolli analitici

    Directory of Open Access Journals (Sweden)

    A. Carducci

    2003-05-01

    Full Text Available

    Obiettivi: la ricerca dei virus enterici in matrici ambientali, soprattutto nei reflui urbani, risulta di grande interesse sia per la valutazione del rischio di natura virale sia per compiere studi epidemiologici.

    Lo scopo di questo lavoro è stato l’applicazione parallela di diverse tecniche attualmente in uso in virologia clinica a isolati citopatogeni ambientali al fine di ottenere una precisa identificazione degli agenti rilevati.

    Metodi: sono stati analizzati 21 campioni di aerosol, provenienti da impianti di depurazione di reflui civili a fanghi attivi, e risultati citopatogeni in colture di cellule BGM.

    Dopo una nuova semina in “shell vials”, su tre linee cellulari continue per mettere in evidenza eventuali differenze nello spettro di citopatogenicità. Per l’identificazione degli isolati virali, sono state adottate in parallelo: microscopia elettronica, tecniche biomolecolari di amplificazione genica, elettroforesi su gel di poliacrilamide (PAGE, test immunoenzimatici, sieroneutralizzazione.

    Risultati: la semina in “shell vials” ha confermato la
    presenza di particelle virali infettive in tutti i campioni
    tranne uno; inoltre ha permesso di ridurre il
    tempo di comparsa dell’effetto citopatico a 3-4 giorni
    contro i 6-8 giorni necessari seminando in fiasche.
    La microscopia elettronica ha individuato la presenza
    di particelle enterovirus-simili in 18 dei 21
    campioni e di virioni della famiglia Reoviridae in 8.
    La positività dei campioni è stata ottenuta, inoltre,
    con la RT-PCR, per 2 campioni di enterovirus e 2 di
    reovirus e con la PAGE in 6 campioni. Il presente
    studio ha portato ad un’identificazione sufficientemente
    certa di reovirus in 2 campioni e di enterovirus
    in 1 campione.

    Conclusioni: l’identificazione di isolati ambientali si

  18. 武陵片区8种水果醇提取物抗氧化活性比较%The Comparison in Antioxidant Activity of Alcohol Extracts from Eight Kinds of Fruit in Wulingshan Region

    Institute of Scientific and Technical Information of China (English)

    唐苗; 李芳; 黄河清; 周小花; 唐申健; 侯娟

    2016-01-01

    比较武陵片区8种新鲜水果的醇提取物的抗氧化活性。采用DPPH法对武陵片区8种新鲜水果醇提取物抗氧化活性的比较。DPPH法测量的结果:这8种水果醇提取物抗氧化活性由强到弱依次是红枣、野葡萄、柑橘,柚子、柿子、猕猴桃、八月瓜、雪莲果。这8种水果都具有较强抗氧化作用,其有效成分值得进一步开发研究,并为以后的水果抗氧化研究提供参考依据。%The DPPH method were used to compare the antioxidant activity of alcohol extracts from eight kinds of fruit in Wulingshan region. Results showed that the antioxidant activity of the eight kinds of fruit of alcohol extracts from strong to weak was in turns as follows: red dates, wild grape, citrus, shaddock, persimmon, kiwifruit, stauntonia latifolia, yacon. All the eight kinds of fruit had stronger antioxidant effect, the effective ingredient was worth further development and research, and reference for the later research on fruit antioxidant activity was provided.

  19. Drought changes the dynamics of trace element accumulation in a Mediterranean Quercus ilex forest

    International Nuclear Information System (INIS)

    We conducted a field drought manipulation experiment in an evergreen oak Mediterranean forest from 1999 to 2005 to investigate the effects of the increased drought predicted for the next decades on the accumulation of trace elements that can be toxic for animals, in stand biomass, litter and soil. Drought increased concentrations of As, Cd, Ni, Pb and Cr in roots of the dominant tree species, Quercus ilex, and leaf Cd concentrations in Arbutus unedo and of Phillyrea latifolia codominant shrubs. The increased concentration of As and Cd can aggravate the toxic capacity of those two elements, which are already next or within the levels that have been shown to be toxic for herbivores. The study also showed a great reduction in Pb biomass content (100-135 g ha-1) during the studied period (1999-2005) showing the effectiveness of the law that prohibited leaded fuel after 2001. The results also indicate that drought increases the exportation of some trace elements to continental waters. - Drought increased biomass concentrations of As and Cd and favors exportation of some trace elements to continental waters in a Mediterranean forest

  20. Chewing sandpaper: grit, plant apparency, and plant defense in sand-entrapping plants.

    Science.gov (United States)

    LoPresti, Eric F; Karban, Richard

    2016-04-01

    Sand entrapment on plant surfaces, termed psammophory or sand armor, is a phylogenetically and geographically widespread trait. The functional significance of this phenomenon has been poorly investigated. Sand and soil are nonnutritive and difficult for herbivores to process, as well as visually identical to the background. We experimentally investigated whether this sand coating physically protected the plant from herbivores or increased crypsis (e.g., decreased apparency to herbivores). We tested the former hypothesis by removing entrapped sand from stems, petioles, and leaves of the sand verbena Abronia latifolia and by supplementing natural sand levels in the honeyscented pincushion plant Navarretia mellita. Consistent with a physical defensive function, leaves with sand present or supplemented suffered less chewing herbivory than those with sand removed or left as is. To test a possible crypsis effect, we coated some sand verbena stems with green sand, matching the stem color, as well as others with brown sand to match the background color. Both suffered less chewing herbivory than controls with no sand and herbivory did not significantly differ between the colors, suggesting crypsis was not the driving resistance mechanism. Strong tests of plant apparency are rare; this experimental approach may be possible in other systems and represents one of few manipulative tests of this long-standing hypothesis. PMID:27220199

  1. Aboriginal uses and management of ethnobotanical species in deciduous forests of Chhattisgarh state in India

    Directory of Open Access Journals (Sweden)

    Kala Chandra

    2009-08-01

    Full Text Available Abstract A study on the native uses of ethnobotanical species was carried out in the south Surguja district of Chhattisgarh state in India with the major objective of identifying different food and medicinal plant species and also to understand their ongoing management and conservation. Through questionnaire and personal interviews, a total of 73 ethnobotanical species used by tribal and non-tribal communities were documented, of these 36 species were used in curing different types of diseases and 22 were used as edible food plants. This rich traditional knowledge of local people has an immense potential for pharmacological studies. The outside forces, at present, were mainly blamed to change the traditional system of harvesting and management of ethnobotanical species. The destructive harvesting practices have damaged the existing populations of many ethnobotanical species viz., Asparagus racemosus, Dioscorea bulbifera, Boswellia serrata, Buchnania lanzan, Sterculia urens and Anogeissus latifolia. The sustainable harvesting and management issues of ethnobotanical species are discussed in view of their conservation and management.

  2. Meiotic studies in some selected angiosperms from the Kashmir Himalayas

    Institute of Scientific and Technical Information of China (English)

    Syed Mudassir JEELANI; Santosh KUMARI; Raghbir Chand GUPTA

    2012-01-01

    As a part of our program to explore and evaluate genetic diversity of flowering plants of the Kashmir Himalayas,meiotic studies have been carried out on 150 wild species.Of these,Caltha alba (2n =32),Delphinium roylei (2n =16),D.uncinatum (2n =16),Ranunculus palmatifidus (2n =28),and Sedum heterodontum (2n =14) have been cytologically worked out for the first time.New intraspecific diploid or polyploid cytotypes have been recorded for Alchemilla vulgaris (2n =34,96),Arabis amplexicaulis (2n =16),Impatiens amphorata (2n =14),Ⅰ.racemosa (2n =12),Ⅰ.sutcata (2n =16,12),Meconopsis latifolia (2n =14),Potentilla supina (2n =14),Saxifraga cernua (2n =16),Sium latijugam (2n =24),and Vicatia coniifolia (2n =44).Four species,Arabidopsis thaliana (2n =10),Berberis vulgaris (2n =28),Potentilla nubicola (2n =14),and P.sericea (2n =28),have been cytologically reported for the first time from India.A large number of meiotic abnormalities have been observed in most of these species,leading to a reduction in pollen fertility and production of heterogeneous-sized pollen grains.

  3. EFICIENCIA DE UN HUMEDAL DE FLUJO SUBSUPERFICIAL PARA EL TRATAMIENTO DE AGUAS RESIDUALES DEL EMPACADO DE HORTALIZAS

    Directory of Open Access Journals (Sweden)

    Amado E. Navarro

    2013-01-01

    Full Text Available Con el objetivo de tratar el agua resultante del pelado de vegetales de una empacadora situada en Atlixco, México, se utilizó un sistema de humedales construidos de flujo subsuperficial horizontal, a nivel de mesocosmos, compuesto por un sedimentador, una celda con grava de río y plantada con Phragmites australis, seguida por dos celdas paralelas, con grava y piedra volcánica, plantadas con Typha latifolia. Operando por semilotes, el tiempo de residencia hidráulica en cada celda fue de 5 días. Las características del agua residual y los resultados del tratamiento fueron altamente variables dependiendo del vegetal procesado. El porcentaje de reducción de la Demanda Química y Bioquímica de Oxígeno (DQO/DBO fue de 77/48 con altas cargas orgánicas del agua residual (DBO > 2000 mg L-1 y de 92/94 con bajas cargas (DBO < 1200 mg L-1. Otros parámetros analizados (pH, conductividad eléctrica, potencial redox, NO2-, NO3- y NH4+ mostraron valores aceptables a la salida del sistema.

  4. Enhanced levels of S-linalool by metabolic engineering of the terpenoid pathway in spike lavender leaves.

    Science.gov (United States)

    Mendoza-Poudereux, Isabel; Muñoz-Bertomeu, Jesús; Navarro, Alicia; Arrillaga, Isabel; Segura, Juan

    2014-05-01

    Transgenic Lavandula latifolia plants overexpressing the linalool synthase (LIS) gene from Clarkia breweri, encoding the LIS enzyme that catalyzes the synthesis of linalool were generated. Most of these plants increased significantly their linalool content as compared to controls, especially in the youngest leaves, where a linalool increase up to a 1000% was observed. The phenotype of increased linalool content observed in young leaves was maintained in those T1 progenies that inherit the LIS transgene, although this phenotype was less evident in the flower essential oil. Cross-pollination of transgenic spike lavender plants allowed the generation of double transgenic plants containing the DXS (1-deoxy-d-xylulose-5-P synthase), coding for the first enzyme of the methyl-d-erythritol-4-phosphate pathway, and LIS genes. Both essential oil yield and linalool content in double DXS-LIS transgenic plants were lower than that of their parentals, which could be due to co-suppression effects linked to the structures of the constructs used.

  5. Repellent Activity of Extracts of Wild Rice Species against Panonychus citri and Aphis citricola in Associated with Esterase Isoenzyme in Insests

    Institute of Scientific and Technical Information of China (English)

    WAN Shu-qing; LIU Xiang-fa; FENG Guo-zhong; PAN Da-jian

    2006-01-01

    Six species of wild rice with different ecophenotypes including Oryza grandiglumis (E6-1, E6-3 / 6-4), O. minuta (E13-9,E13-13), O. officinalis (E15-8, E15-13), O. punctata (E16-1, E16-3, E1 6-13), O. granulata (E7-4), and O. latifolia (101392, E9-1, E9-10)were extracted with methnol and the repellent activity of the extracts against the two insects Aphis citricola and Panonychus citri were studied. The extracts of O. officinalis E15-8 showed higher repellent rate to the two insects than those of the other species. The repellent rates of the extracts of E15-8 to P. citriand A. citricola were 83.26% and 87.86% at 5×104 μg/mL in 24 h and 87.95% and 82.43% in 48 h, respectively. The extracts of O. officinalis E15-8 had the effect of inhibition to the esterase of the two insects.

  6. Perfil químico y biológico de aceites esenciales de plantas aromáticas de interés agro-industrial en Castilla-La Mancha (España

    Directory of Open Access Journals (Sweden)

    Santana, O.

    2012-06-01

    Full Text Available The chemical composition and biological activities of essential oils of Salvia officinalis L., Salvia lavandulifolia Vahl., Lavandula x intermedia Emeric ex Loisel., Lavandula latifolia Medik., Lavandula angustifolia Mill. and Thymus vulgaris L. are presented. The essential oils have been analysed by Gas Chromatography Mass Spectrometry and 61 compounds were identified, 23 of which represented more than 1% of the essential oil. The 1,8 cineole (16-23% appeared as the main compound of Salvia sp. essential oils. The high content of α-thujone was characteristic in S. officinalis oil. Remarkable concentrations of linalool (30-33%, camphor (5-17% and linalyl acetate (9-28% were detected in Lavandula sp. oils while carvacrol (21.6% and p-cimene (23.7% were the most abundant compounds in T. vulgaris oil. Biological characterization was based on their bioplaguicide activity. The essential oils studied had strong antifeedant effects against Leptinotarsa decemlineata Say, Spodoptera littoralis Boisd., Myzus persicae Sulzer and Rhopalosiphum padi L., phytotoxic activity against Lactuca sativa L. and Lolium perenne L. and also exhibited high antifungal activity against Fusarium sp. Oils from T. vulgaris and L. latifolia showed the highest levels of bioactivity against all target species. These results provide an added-value to the essential oils of aromatic plants of agro-industrial interest for its potential use in the development of natural agrochemicals.

    En este trabajo se presenta el estudio químico y biológico de los aceites esenciales de Salvia officinalis L., Salvia lavandulifolia Vahl., Lavandula x intermedia Emeric ex Loisel., Lavandula latifolia Medik., Lavandula angustifolia Mill. y Thymus vulgaris L. El estudio químico por cromatografía de gases acoplada a

  7. Assessment of native plant species for phytoremediation of heavy metals growing in the vicinity of NTPC sites, Kahalgaon, India.

    Science.gov (United States)

    Kumari, Alka; Lal, Brij; Rai, Upendra Nath

    2016-01-01

    The present investigation was carried out to screen native plants growing in fly ash (FA) contaminated areas near National Thermal Power Corporation (NTPC), Kahalgaon, Bihar, India with a view to using them for the eco-restoration of the area. A total number of 30 plant species (5 aquatic and 25 terrestrial including 6 ferns) were collected and their diversity status and dominance were also studied. After screening of dominant species at highly polluted site, 8 terrestrial and 5 aquatic plants were analyzed for heavy metals (Fe, Zn, Cu, Ni, Si, Al, Pb, Cr, and Cd). Differential accumulations of various heavy metals by different species of plants were observed. Typha latifolia was found to be most efficient metal accumulator of Fe (927), Cu (58), Zn (87), Ni (57), Al (67), Cd (95), and Pb (69), and Azolla pinnata as Cr (93) hyper-accumulator among aquatic species in µg g(-1). In terrestrial species the maximum levels of Fe (998), Zn (81), Ni (93), Al (121), and Si (156) were found in Croton bonplandium. However, there was high spatial variability in total metal accumulation in different species indicated by coefficient of variation (CV%). These results suggest that various aquatic, some dominant terrestrial plants including fern species may be used in a synergistic way to remediate and restore the FA contaminated wastelands. PMID:26442874

  8. Hybrid pine for tough sites

    International Nuclear Information System (INIS)

    A test planting of 30 first- and second-generation pitch x loblolly pine (pinus rigida x P. taeda) hybrids was established on a West Virginia minesoil in 1985. The site was considered orphaned because earlier attempts at revegetation were unsuccessful. The soil was acid (pH 4.6), lacking in nutrients, and compacted. Vegetation present at the time of planting consisted of a sparse cover of tall fescue (Festuca arundinacea) and poverty grass (Danthonia spicata) and a few sourwood (Oxydendrum arboreum) and mountain laurel (Kalmia latifolia) seedlings. In the planting trial, 30 different hybrids were set out in 4 tree linear plots replicated 5 times. The seedlings had been grown in containers for 1 yr before outplanting. Evaluations made after 6 growing seasons showed overall plantation survival was 93%; six hybrids and one open-pollinated cross survived 100%. Individual tree heights ranged from 50 to 425 cm with a plantation average of 235 cm (7.7 ft). Eleven of the hybrids had average heights that exceeded the plantation average. Another test planting of tree and shrub species on this site has very poor survival. Therefore, pitch x loblolly hybrid pine can be recommended for reclaiming this and similar sites

  9. Characterizing the physical and genetic structure of the lodgepole pine × jack pine hybrid zone: mosaic structure and differential introgression.

    Science.gov (United States)

    Cullingham, Catherine I; James, Patrick M A; Cooke, Janice E K; Coltman, David W

    2012-12-01

    Understanding the physical and genetic structure of hybrid zones can illuminate factors affecting their formation and stability. In north-central Alberta, lodgepole pine (Pinus contorta Dougl. ex Loud. var. latifolia) and jack pine (Pinus banksiana Lamb) form a complex and poorly defined hybrid zone. Better knowledge of this zone is relevant, given the recent host expansion of mountain pine beetle into jack pine. We characterized the zone by genotyping 1998 lodgepole, jack pine, and hybrids from British Columbia, Alberta, Saskatchewan, Ontario, and Minnesota at 11 microsatellites. Using Bayesian algorithms, we calculated genetic ancestry and used this to model the relationship between species occurrence and environment. In addition, we analyzed the ancestry of hybrids to calculate the genetic contribution of lodgepole and jack pine. Finally, we measured the amount of gene flow between the pure species. We found the distribution of the pine classes is explained by environmental variables, and these distributions differ from classic distribution maps. Hybrid ancestry was biased toward lodgepole pine; however, gene flow between the two species was equal. The results of this study suggest that the hybrid zone is complex and influenced by environmental constraints. As a result of this analysis, range limits should be redefined. PMID:23346232

  10. Extractor capacity of different plant species cultivated in wetlands used to pig wastewater treatment

    Directory of Open Access Journals (Sweden)

    Antonio Teixeira de Matos

    2009-08-01

    Full Text Available The objective of this study was to evaluate the extracting capacity of different plant species when cultivated in constructed wetlands systems (CWS for the treatment of pig wastewaters (PW. For this, four CWS were constructed with 24.0 m x 1.1 m x 0.7 m, sealed with geomembrana of polyvinyl chloride (PVC and filled with 0.4 m of gravel “zero”. In CWS1, CWS2 and CWS3 were planted cattail (Typha latifolia L., Alternanthera philoxeroides (Mart. Griseb. and grass-Tifton 85 (Cynodon dactylon Pers., respectively. In CWS4 was planted Alternanthera on the 1st third, Typha in 2nd third and tifton-85 in the 3rd third of the bed. After passing through a organic filter filled with crushed sugar cane bagasse, the ARS was applied in SACS in a flow of 0.8 m3 d-1, which provided a detention time of 4.8 days. There was a trend to obtain higher extraction of pollutants by plants grown at the beginning of the CWS. The Alternanthera plant species that was presented greater capacity for nutrient extractor, extracting 9.5 and 23% of all total-N and K applied through ARS. Plants extracted small amounts of copper from the ARS. Because of the improved performance of plants, Alternanthera or Tifton-85 grass must be cultivated in CWS for the ARS treatment.

  11. О НЕКОТОРЫХ АССОЦИАЦИЯХ ПРИБРЕЖНО-ВОДНОЙ И БОЛОТНОЙ РАСТИТЕЛЬНОСТИ КУРСКОЙ ОБЛАСТИ

    OpenAIRE

    Полуянов, А.

    2008-01-01

    Дана характеристика трех новых для Курской области ассоциаций прибрежноводной и болотной растительности: Equisetetum fluviatilis Steffen 1931, Phragmitetum communis Savich 1926 и Typhetum latifoliae (Soo 1927) Long 1973. Ассоциации относятся к союзу Phragmition communis Koch 1926 порядка Phragmitetalia Koch 1926 класса PhragmitiMagnocaricetea Klika in Klika et Novak 1941. Сообщества ассоциаций широко распространены по территории области....

  12. Drought tolerance and growth in populations of a wide-ranging tree species indicate climate change risks for the boreal north.

    Science.gov (United States)

    Montwé, David; Isaac-Renton, Miriam; Hamann, Andreas; Spiecker, Heinrich

    2016-02-01

    Choosing drought-tolerant planting stock in reforestation programs may help adapt forests to climate change. To inform such reforestation strategies, we test lodgepole pine (Pinus contorta Doug. ex Loud. var latifolia Englm.) population response to drought and infer potential benefits of a northward transfer of seeds from drier, southern environments. The objective is addressed by combining dendroecological growth analysis with long-term genetic field trials. Over 500 trees originating from 23 populations across western North America were destructively sampled in three experimental sites in southern British Columbia, representing a climate warming scenario. Growth after 32 years from provenances transferred southward or northward over long distances was significantly lower than growth of local populations. All populations were affected by a severe natural drought event in 2002. The provenances from the most southern locations showed the highest drought tolerance but low productivity. Local provenances were productive and drought tolerant. Provenances from the boreal north showed lower productivity and less drought tolerance on southern test sites than all other sources, implying that maladaptation to drought may prevent boreal populations from taking full advantage of more favorable growing conditions under projected climate change. PMID:26463121

  13. Usefulness of different vascular plant species for passive biomonitoring of Mediterranean rivers.

    Science.gov (United States)

    Baldantoni, Daniela; Alfani, Anna

    2016-07-01

    Choosing native vascular plants as nutrient and toxic element accumulators for passive biomonitoring of urban river quality is not an easy task in Mediterranean rivers, due to the particular climate determining high variations in river hydrology. To identify potential biomonitors for this area, the roots of seven species (Angelica sylvestris, Apium nodiflorum, Tradescantia fluminensis, Nasturtium officinale, Persicaria lapathifolia, Arctium lappa, Typha latifolia), growing in seven sites along the River Irno (Southern Italy), were collected in July 2010 and analyzed regarding their capability to accumulate Cd, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, V, and Zn through atomic absorption spectrometry. Notwithstanding the expected different accumulation degree among the species, they highlighted similar spatial contamination gradients, and all of them appeared suitable, alone or in combination, for river passive biomonitoring. A. nodiflorum, in particular, appeared the best biomonitor for the River Irno, where severe anthropogenic impacts were detected: high Cu and Cd contamination from vine cultivation in the upper stretch, and Pb, Zn, and Mn contamination in the medium stretch from airborne dusts coming from a cast iron foundry. PMID:27040538

  14. Naturalistic Engineering for risk prevention in two slopes in southern Quito

    Directory of Open Access Journals (Sweden)

    Anita Argüello

    2013-12-01

    Full Text Available (Received: 2013/11/01 - Accepted: 2013/12/12Naturalistic engineering is a technical-scientific modern science cobining civil, environmental and geotechnical engineerings. It studies and uses building materials, plants, organic and synthetic materials for holding slopes. San Luis de Chillogallo and El Recreo are located in the South of Quito, where two projects for erosion control, containment and environmental recovery, have been implemented. These are pilot interventions that allow applying strategies and capabilities of estimation and reduction of risks from disasters. To implement the works, the ground was shifted, the organic and inorganic matter was wiped out, and unstable parts of the slope were removed, reshaping the slope through land exclusion and relocation. Subsequently, depending on the shape of each slope, specific techniques where designed and implemented. Double Wall Crib and Latin Triangular Branching techniques were used in San Luis de Chillogallo. Live Grating and Latino Triangular Branching techniques were used in El Recreo. Plants such as: Alder, Alnus glutinosa; paper tree, Polylepis sp.; chilca, Baccharis latifolia; lechero Euphorbia lactiflua and Tilo, Tilia platyphyllos; have been used in these projects. These plants are fast growing species and they have adapted successfully on the two slopes intervened.

  15. Root distribution of rootstocks for 'Tahiti' lime

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    Neves Carmen Silvia Vieira Janeiro

    2004-01-01

    Full Text Available Field studies on citrus roots are important for genetic selection of cultivars and for management practices such as localized irrigation and fertilization. To characterize root systems of six rootstocks, taking into consideration chemical and physical characteristics of a clayey Typic Hapludox of the Northern State of Paraná, this study was performed having as scion the 'IAC-5 Tahiti' lime [Citrus latifolia (Yu. Tanaka]. The rootstocks 'Rangpur' lime (C. limonia Osbeck, 'Africa Rough' lemon (C. jambhiri Lush., 'Sunki' mandarin [C. sunki (Hayata hort. ex Tan.], Poncirus trifoliata (L. Raf., 'C13' citrange [C. sinensis (L. Osb. x P. trifoliata (L. Raf] and 'Catânia 2' Volkamer lemon (C. volkameriana Ten. & Pasq. were used applying the trench profile method and the SIARCS® 3.0 software to determine root distribution. 'C-13' citrange had the largest root system. 'Volkamer' lemon and 'Africa Rough' lemon presented the smallest amount of roots. The effective depth for 80 % of roots was 31-53 cm in rows and 67-68 cm in inter-rows. The effective distance of 80 % of roots measured from the tree trunk exceeded the tree canopy for P. trifoliata, 'Sunki' mandarin, and 'Volkamer' and 'Africa Rough' lemons.

  16. Influence of different plant species on methane emissions from soil in a restored Swiss wetland.

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    Gurbir S Bhullar

    Full Text Available Plants are a major factor influencing methane emissions from wetlands, along with environmental parameters such as water table, temperature, pH, nutrients and soil carbon substrate. We conducted a field experiment to study how different plant species influence methane emissions from a wetland in Switzerland. The top 0.5 m of soil at this site had been removed five years earlier, leaving a substrate with very low methanogenic activity. We found a sixfold difference among plant species in their effect on methane emission rates: Molinia caerulea and Lysimachia vulgaris caused low emission rates, whereas Senecio paludosus, Carex flava, Juncus effusus and Typha latifolia caused relatively high rates. Centaurea jacea, Iris sibirica, and Carex davalliana caused intermediate rates. However, we found no effect of either plant biomass or plant functional groups--based on life form or productivity of the habitat--upon methane emission. Emissions were much lower than those usually reported in temperate wetlands, which we attribute to reduced concentrations of labile carbon following topsoil removal. Thus, unlike most wetland sites, methane production in this site was probably fuelled chiefly by root exudation from living plants and from root decay. We conclude that in most wetlands, where concentrations of labile carbon are much higher, these sources account for only a small proportion of the methane emitted. Our study confirms that plant species composition does influence methane emission from wetlands, and should be considered when developing measures to mitigate the greenhouse gas emissions.

  17. Essential oils and distilled straws of lavender and lavandin: a review of current use and potential application in white biotechnology.

    Science.gov (United States)

    Lesage-Meessen, Laurence; Bou, Marine; Sigoillot, Jean-Claude; Faulds, Craig B; Lomascolo, Anne

    2015-04-01

    The Lavandula genus, which includes lavender (Lavandula angustifolia) and lavandin (L. angustifolia × Lavandula latifolia), is cultivated worldwide for its essential oils, which find applications in perfumes, cosmetics, food processing and, more recently, in aromatherapy products. The chemical composition of lavender and lavandin essential oils, usually produced by steam distillation from the flowering stems, is characterized by the presence of terpenes (e.g. linalool and linalyl acetate) and terpenoids (e.g. 1,8-cineole), which are mainly responsible for their characteristic flavour and their biological and therapeutic properties. Lavender and lavandin distilled straws, the by-products of oil extraction, were traditionally used for soil replenishment or converted to a fuel source. They are mineral- and carbon-rich plant residues and, therefore, a cheap, readily available source of valuable substances of industrial interest, especially aroma and antioxidants (e.g. terpenoids, lactones and phenolic compounds including coumarin, herniarin, α-bisabolol, rosmarinic and chlorogenic acids). Accordingly, recent studies have emphasized the possible uses of lavender and lavandin straws in fermentative or enzymatic processes involving various microorganisms, especially filamentous fungi, for the production of antimicrobials, antioxidants and other bioproducts with pharmaceutical and cosmetic activities, opening up new challenging perspectives in white biotechnology applications. PMID:25761625

  18. Simulation of metabolism-based herb-drug interaction: towards safe and efficacious use of NIPRD-AM1

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    Bulus Adzu

    2013-05-01

    Full Text Available Objective: To evaluate the effect of NIPRD-AM1 on CYP3A4 in order to generate clinically significant data for its safe and efficacious use. Materials and Methods: NIPRD-AM1 is a phytomedicine developed from aqueous root extracts of Nauclea latifolia Smith (Rubiaceae for the treatment of uncomplicated malaria. The effect of NIPRD-AM1 on CYP3A4 was measured with and without the addition of NIPRD-AM1, by testing different concentrations of the product at 37 °C in reactive mixtures with ketoconazole (2.5 µM as the positive control. Results: Results showed a very low IC50 value of 0.01 mg/ml similar to that of ketoconazole (0.016 mg/ml. Conclusion: Metabolic processes of NIPRD-AM1 are likely to inhibit CYP3A4, with potential implication on drugs that are CYP3A4 substrates. This is a promising approach for guidance towards the safe and efficacious use of NIPRD-AM1.

  19. Simulation of metabolism-based herb-drug interaction: towards safe and efficacious use of NIPRD-AM1

    Science.gov (United States)

    Adzu, Bulus; Mustapha, Kudirat Bola; Masimirembwa, Collen; Obodozie, Obiageri; Kirim, Rukaiyatu Abdullahi; Gamaniel, Karniyus Shingu

    2013-01-01

    Objective: To evaluate the effect of NIPRD-AM1 on CYP3A4 in order to generate clinically significant data for its safe and efficacious use. Materials and Methods: NIPRD-AM1 is a phytomedicine developed from aqueous root extracts of Nauclea latifolia Smith (Rubiaceae) for the treatment of uncomplicated malaria. The effect of NIPRD-AM1 on CYP3A4 was measured with and without the addition of NIPRD-AM1, by testing different concentrations of the product at 37 °C in reactive mixtures with ketoconazole (2.5 µM) as the positive control. Results: Results showed a very low IC50 value of 0.01 mg/ml similar to that of ketoconazole (0.016 mg/ml). Conclusion: Metabolic processes of NIPRD-AM1 are likely to inhibit CYP3A4, with potential implication on drugs that are CYP3A4 substrates. This is a promising approach for guidance towards the safe and efficacious use of NIPRD-AM1. PMID:25050275

  20. A corm-specific gene encodes tarin, a major globulin of taro (Colocasia esculenta L. Schott).

    Science.gov (United States)

    Bezerra, I C; Castro, L A; Neshich, G; de Almeida, E R; de Sá, M F; Mello, L V; Monte-Neshich, D C

    1995-04-01

    A gene encoding a globulin from a major taro (Colocasia esculenta L. Schott) corm protein family, tarin (G1, ca. 28 kDa) was isolated from a lambda Charon 35 library, using a cDNA derived from a highly abundant corm-specific mRNA, as probe. The gene, named tar1, and the corresponding cDNA were characterized and compared. No introns were found. The major transcription start site was determined by primer extension analysis. The gene has an open reading frame (ORF) of 765 bp, and the deduced amino acid sequence indicated a precursor polypeptide of 255 residues that is post-translationally processed into two subunits of about 12.5 kDa each. The deduced protein is 45% homologous to curculin, a sweet-tasting protein found in the fruit pulp of Curculigo latifolia and 40% homologous to a mannose-binding lectin from Galanthus nivalis. Significant similarity was also found at the nucleic acid sequence level with genes encoding lectins from plant species of the Amaryllidaceae and Lilliaceae families.

  1. 10种湿地水生经济植物综合评价%Comprehensive Evaluation on Ten Kinds of Aquatic Economic Plants in Wetlands

    Institute of Scientific and Technical Information of China (English)

    熊汉锋; 彭有轩; 孙海元; 史玉虎; 吴翠

    2009-01-01

    选取了6个评价因子,采用实地调查分析和层次分析方法对长江流域的10种湿地水生经济植物进行了经济功能和生态功能的综合评价.结果表明:综合效益最好的是莲藕,其次为茭白、藜蒿、水芹、芡实、荸荠、菱角、芦苇、水稻,综合效益最差的是芋头;实际测定值与单因子权重排序一致.%Six evaluation factors were chosen. The economic and ecological functions of ten aquatic economic plants in wetlands of Yangtze River Basin were evaluated comprehensively by adopting the methods of field survey and Analytical Hierarchy Process. The results showed that Nelumbo nucifeya Gaertn had the best comprehensive benefit, followed by Zizania latifolia, Artemisia selengensis Turcz, Oenanthe javanica, Euryale ferox Salisb, Eleocharis dulcis, Trapa bicornis, Phragmites australis, Oryza sativa and Colocasia esculenta . The rank of actual measured value was in accordance with weight of single factor.

  2. CRECIMIENTO Y PRODUCTIVIDAD DE CHAYA (Cnidoscolus chayamansa MCVAUGH, EUPHORBIACEAE CON DENSIDAD DE PLANTACIÓN VARIABLE

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    Jesús Mao E. Aguilar Luna

    2011-01-01

    Full Text Available Se evaluó el efecto que tiene la competenciainterespecífica en el crecimiento y la productividad de la chayacon densidad de plantación (DP variable, en un suelocalcimórfico de tipo rendzina negra en Noh-Bec, Quintana Roo,México. La chaya se estableció a 1.50 x 3.00 m, utilizando estacassin hojas; se asoció con árboles jóvenes (menores de dosaños de Cedrela odorata y Citrus latifolia en un diseño deplantación en círculo 'Nelder' de 3154 m2. Se establecieron ochoDP de 2602 a 3772 plantas.ha-1 con 10 repeticiones. Lasvariables de respuesta fueron: capacidad de enraizamiento,tasa de crecimiento, índice de vigor, índice de área foliar yproducción de biomasa. El mayor enraizamiento de estacas dechaya se obtuvo de 2889 a 3772 plantas.ha-1; sin embargo, elmejor crecimiento y productividad se obtuvo de 2706 a2889 plantas.ha-1.

  3. Floral development in Tribe Detarieae (Leguminosae: Caesalpinioideae): Amherstia, Brownea, and Tamarindus.

    Science.gov (United States)

    Tucker, S C

    2000-10-01

    Floral development was compared among three taxa in caesalpinioid tribe Detarieae sensu lato: Amherstia nobilis and Tamarindus indica have racemose, helically arranged inflorescences, while Brownea latifolia has cauliflorous capitate flower clusters that arise as racemes. All have acropetal flower order; initiation and development are sequential in all except Brownea, which is synchronous. All have paired persistent showy bracteoles. Floral symmetry is dorsiventral (zygomorphic) in all except Brownea, with radial symmetry at anthesis. Sepals initiate helically on a circular floral apex, starting with a median abaxial sepal, in all. Petals are initiated helically in Brownea, and unidirectionally in Amherstia and Tamarindus. Stamens are initiated unidirectionally in each stamen whorl in all except Amherstia, in which the outer whorl is bidirectional. The carpel initiates concurrently with the petals in Brownea, and with the outer stamens in the other taxa. The two upper (adaxial) sepal primordia become fused during development in all, so that the calyx appears tetramerous. Some reduced petals occur in Amherstia and Tamarindus, and some reduced stamens occur in all. All produce a hypanthium by zonal growth, and all except Tamarindus have the gynoecium attached adaxially to the hypanthial rim. PMID:11034916

  4. Growth and metabolism of Typha species in relation to cutting treatments

    Energy Technology Data Exchange (ETDEWEB)

    Sale, P.J.M.; Wetzel, R.G.

    1983-01-01

    Container and field experiments, in which Typha latifolia L. and Typha angustifolia L. were cut either above or below the water level, were conducted to determine the physiological basis for reports that the latter treatment was more effective as a control measure. In containers, measurements of oxygen concentrations within the aerenchyma of the rhizome both with an oxygen electrode and by gas chromatography showed that oxygen could diffuse very readily to plant parts growing in an anoxic environment if there was a small amount of leaf or cut plant stem growing above the water level. When all shoots were cut below water, the oxygen in submersed plant parts was rapidly consumed and anaerobic respiration resulted in the production of ethanol. Lactate or elevated malate levels were not found. The below-water biomass decayed rapidly under these conditions and the plants had a much lower regenerative ability than plants cut above water where oxygen continued to reach the roots and rhizomes. In the field, three cuts during the growing season below water were sufficient to kill nearly all the underwater biomass; similar cuts above water reduced the total biomass compared with uncut plants, but much of the underwater biomass remained healthy and able to regenerate. 21 references, 2 figures, 5 tables.

  5. Sulfate removal and sulfur transformation in constructed wetlands: The roles of filling material and plant biomass.

    Science.gov (United States)

    Chen, Yi; Wen, Yue; Zhou, Qi; Huang, Jingang; Vymazal, Jan; Kuschk, Peter

    2016-10-01

    Sulfate in effluent is a challenging issue for wastewater reuse around the world. In this study, sulfur (S) removal and transformation in five batch constructed wetlands (CWs) treating secondary effluent were investigated. The results showed that the presence of the plant cattail (Typha latifolia) had little effect on sulfate removal, while the carbon-rich litter it generated greatly improved sulfate removal, but with limited sulfide accumulation in the pore-water. After sulfate removal, most of the S was deposited with the valence states S (-II) and S (0) on the iron-rich gravel surface, and acid volatile sulfide was the main S sink in the litter-added CWs. High-throughput pyrosequencing revealed that sulfate-reducing bacteria (i.e. Desulfobacter) and sulfide-oxidizing bacteria (i.e. Thiobacillus) were dominant in the litter-added CWs, which led to a sustainable S cycle between sulfate and sulfide. Overall, this study suggests that recycling plant litter and iron-rich filling material in CWs gives an opportunity to utilize the S in the wastewater as both an electron acceptor for sulfate reduction and as an electron donor for nitrate reduction coupled with sulfide oxidation. This leads to the simultaneous removal of sulfate, nitrate, and organics without discharging toxic sulfide into the receiving water body. PMID:27423407

  6. [Selection and purification potential evaluation of woody plant in vertical flow constructed wetlands in the subtropical area].

    Science.gov (United States)

    Chen, Yong-Hua; Wu, Xiao-Fu; Hao, Jun; Chen, Ming-Li; Zhu, Guang-Yu

    2014-02-01

    In order to solve the problem that wetland herbaceous plants tend to die during winter in subtropics areas, selection and purification potential evaluation experiments were carried out by introducing into the constructed wetlands 16 species of woody wetland plants. Cluster analysis was performed by including the morphological characteristics, physiological characteristics, as well as nitrogen and phosphorus accumulation of the woody wetland plants. The results indicated that there were significant differences among the tested woody plants in their survival rate, height increase, root length increase and vigor, Chlorophyll content, Superoxide dismutase, Malonaldehyde, Proline, Peroxidase, biomass, average concentration and accumulation of nitrogen and phosphorus. Based on the established evaluation system, the tested plants were clustered into 3 groups. The plants in the 1st group possessing high purification potentials are Nerium oleander and Hibiscus syriacus. Those in the 2nd group possessing moderate purification potentials are Trachycarpus fortune, Llex latifolia Thunb., Gardenia jasminoides, Serissa foetida and Ilex crenatacv Convexa. And those in the 3rd group with low purification potentials are Jasminum udiflorum, Hedera helix, Ligustrum vicaryi, Ligustrum lucidum, Buxus sempervives, Murraya paniculata, Osmanthus fragrans, Mahoniafortune and Photinia serrulata. PMID:24812951

  7. Study on Orchid Diversity in Gunung Simpang Nature Reserve, West Java

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    DWI MURTI PUSPITANINGTYAS

    2005-04-01

    Full Text Available Gunung Simpang Nature Reserve is located in West Java Province. It covers a 15.000 hectare area of highland forest, the altitude range between 800 to 1823 m asl. Orchid inventory and exploration were conducted to study orchid diversity in this conservation areas. Living plant was collected for ex situ conservation purpose in Botanic Garden. Observation on the population of terrestrial and epiphyte orchid was also done to study the dominant orchid in that area. It was recorded that there were 137 orchid species belonging to 51 genera, 95 species of which were epiphytes and 42 other species were terrestrial orchids. 134 species of which were collected in Cibodas Botanic Garden, which is suitable place for highland plant. The most dominant terrestrial orchid was Plocoglottis javanica. Other species were also abundantly found, such as Phaius pauciflorus, Liparis rheedii, Diglyphosa latifolia, Neuwiedia zollingeri var. javanica, Calanthe ceciliae, C. speciosa and Phaius callosus. Some epiphyte orchids were very common found, that are Agrostophyllum majus, Coelogyne speciosa, Dendrobium mutabile, Agrostophyllum bicuspidatum, Pholidota ventricosa and Eria javanica. Some attractive orchids are potential for ornamental plant, such as Vanda tricolor, Phaius callosus, Phaius tankervilleae, Arundina graminifolia, Bulbophyllum lobbii, Coelogyne speciosa, Calanthe ceciliae, Calanthe triplicata and Calanthe speciosa.

  8. Screening the wetland plant species Alisma plantago-aquatica, Carex rostrata and Phalaris arundinacea for innate tolerance to zinc and comparison with Eriophorum angustifolium and Festuca rubra Merlin

    Energy Technology Data Exchange (ETDEWEB)

    Matthews, David J. [Wetland Ecology Research Group, Department of Botany, University College Dublin, Belfield, Dublin 4 (Ireland)]. E-mail: davematt00@hotmail.com; Moran, Bridget M. [Wetland Ecology Research Group, Department of Botany, University College Dublin, Belfield, Dublin 4 (Ireland); Otte, Marinus L. [Wetland Ecology Research Group, Department of Botany, University College Dublin, Belfield, Dublin 4 (Ireland)

    2005-03-01

    Several wetland plant species appear to have constitutive metal tolerance. In previous studies, populations from contaminated and non-contaminated sites of the wetland plants Typha latifolia, Phragmites australis, Glyceria fluitans and Eriophorum angustifolium were found to be tolerant to high concentrations of metals. This study screened three other species of wetland plants: Alisma plantago-aquatica, Carex rostrata and Phalaris arundinacea for innate tolerance to zinc. The degree of tolerance was compared to known zinc-tolerant E. angustifolium and Festuca rubra Merlin. It was found that A. plantago-aquatica and P. arundinacea did not posses innate tolerance to zinc, but that C. rostrata was able to tolerate elevated levels of zinc, at levels comparable to those tolerated by E. angustifolium and F. rubra Merlin. The findings support the theory that some wetland angiosperm species tend to be tolerant to exposure to high levels of metals, regardless of their origin. - Some wetland angiosperms are tolerant to high concentrations of metals, regardless of conditions in the plants' natural habitat.

  9. Review of potential environmental impacts of transgenic glyphosate-resistant soybean in Brazil.

    Science.gov (United States)

    Cerdeira, Antonio L; Gazziero, Dionsio L P; Duke, Stephen O; Matallo, Marcus B; Spadotto, Claudio A

    2007-01-01

    Transgenic glyphosate-resistant soybeans (GRS) have been commercialized and grown extensively in the Western Hemisphere, including Brazil. Worldwide, several studies have shown that previous and potential effects of glyphosate on contamination of soil, water, and air are minimal, compared to those caused by the herbicides that they replace when GRS are adopted. In the USA and Argentina, the advent of glyphosate-resistant soybeans resulted in a significant shift to reduced- and no-tillage practices, thereby significantly reducing environmental degradation by agriculture. Similar shifts in tillage practiced with GRS might be expected in Brazil. Transgenes encoding glyphosate resistance in soybeans are highly unlikely to be a risk to wild plant species in Brazil. Soybean is almost completely self-pollinated and is a non-native species in Brazil, without wild relatives, making introgression of transgenes from GRS virtually impossible. Probably the highest agricultural risk in adopting GRS in Brazil is related to weed resistance. Weed species in GRS fields have shifted in Brazil to those that can more successfully withstand glyphosate or to those that avoid the time of its application. These include Chamaesyce hirta (erva-de-Santa-Luzia), Commelina benghalensis (trapoeraba), Spermacoce latifolia (erva-quente), Richardia brasiliensis (poaia-branca), and Ipomoea spp. (corda-de-viola). Four weed species, Conyza bonariensis, Conyza Canadensis (buva), Lolium multiflorum (azevem), and Euphorbia heterophylla (amendoim bravo), have evolved resistance to glyphosate in GRS in Brazil and have great potential to become problems. PMID:17562462

  10. Comportamento de leguminosas (Adesmia, Lotus, Trifolium em mistura com festuca Response of legumes (Adesmia, Lotus, Trifolium in mixture with tall fescue

    Directory of Open Access Journals (Sweden)

    Simone Meredith Scheffer-Basso

    2002-11-01

    Full Text Available A introdução de leguminosas hibernais é uma das práticas mais importantes para incrementar a quantidade, a qualidade e a sustentabilidade de pastagens perenes no Sul do Brasil. Durante os anos de 2000 e 2001, foi avaliado o comportamento de seis leguminosas temperadas (Adesmia latifolia - nativa, Lotus corniculatus, L. subbiflorus, L. uliginosus e Trifolium repens - cv. Yi e Regal, em mistura com festuca (Festuca arundinacea. Entre a semeadura e o último corte decorreram 475 dias, sendo realizados seis cortes: no outono-inverno/2000, foi feito um corte; na primavera-verão, três e, no outono-inverno/2001, dois cortes. Os intervalos entre cortes variaram entre 43 a 91 dias, sendo menores na estação estival. No total do período experimental obtiveram-se, na média das misturas, 15.038 kg/ha de MS, sendo que, na primavera-verão foram produzidos cerca de 54% desse total, com taxa de crescimento média de 46 kg/ha/dia de MS. Nos meses de outono-inverno de 2000 e 2001, foram obtidas taxas de 22 e 24 kg/ha/dia de MS. O cornichão e o trevo-branco cv. Yi foram as leguminosas mais produtivas, sendo que a primeira destacou-se na primavera-verão (3.500 kg/ha de MS e a última participou com 86,3% na mistura no outono-inverno/2001, com 2.300 kg/ha de MS. As misturas contendo essas leguminosas apresentaram a menor quantidade de invasoras e produziram, respectivamente, 13.663 e 11.184 kg/ha de MS, sendo 82 e 71% desses totais compostos das leguminosas, festuca e azevém. A. latifolia não teve bom estabelecimento e sua participação foi de 0,84% no primeiro corte. L. subbiflorus teve uma boa participação no primeiro ano (37%, mas desapareceu no segundo ano. L. uliginosus mostrou boa persistência, produzindo 1.400 kg/ha de MS no outono/2001.Overseeding temperate legumes is one of the most practices to increase the quantity, quality and sustainability of perennial pastures in Southern Brazil. During the years of 2000 and 2001 the response of six

  11. Phytoremediation of imazalil and tebuconazole by four emergent wetland plant species in hydroponic medium.

    Science.gov (United States)

    Lv, Tao; Zhang, Yang; Casas, Mònica E; Carvalho, Pedro N; Arias, Carlos A; Bester, Kai; Brix, Hans

    2016-04-01

    Pollution from pesticide residues in aquatic environments is of increasing concern. Imazalil and tebuconazole, two commonly used systemic pesticides, are water contaminants that can be removed by constructed wetlands. However, the phytoremediation capability of emergent wetland plants for imazalil and tebuconazole, especially the removal mechanisms involved, is poorly understood. This study compared the removal of both pesticides by four commonly used wetland plants, Typha latifolia, Phragmites australis, Iris pseudacorus and Juncus effusus, and aimed to understand the removal mechanisms involved. The plants were individually exposed to an initial concentration of 10 mg/L in hydroponic solution. At the end of the 24-day study period, the tebuconazole removal efficiencies were relatively lower (25%-41%) than those for imazalil (46%-96%) for all plant species studied. The removal of imazalil and tebuconazole fit a first-order kinetics model, with the exception of tebuconazole removal in solutions with I. pseudacorus. Changes in the enantiomeric fraction for imazalil and tebuconazole were detected in plant tissue but not in the hydroponic solutions; thus, the translocation and degradation processes were enantioselective in the plants. At the end of the study period, the accumulation of imazalil and tebuconazole in plant tissue was relatively low and constituted 2.8-14.4% of the total spiked pesticide in each vessel. Therefore, the studied plants were able to not only take up the pesticides but also metabolise them.

  12. Research progress in the phytochemistry and biology of Ilex pharmaceutical resources

    Directory of Open Access Journals (Sweden)

    Dacheng Hao

    2013-02-01

    Full Text Available Ilex is a botanical source for various health-promoting and pharmaceutically active compounds that have been used in traditional Chinese medicine and food for thousands of years. Increasing interest in Ilex pharmaceutical and food resources has led to additional discoveries of terpenoids, saponins, polyphenols (especially flavonoids, glycosides, and many other compounds in various Ilex species, and to investigation of their chemotaxonomy, molecular phylogeny and pharmacology. In continuation of our studies on Ilex pharmacology and phylogeny, we review the phytochemistry, chemotaxonomy, molecular biology and phylogeny of Ilex species and their relevance to health-promotion and therapeutic efficacy. The similarity and dissimilarity between Ilex paraguariensis, the source plant of mate tea, and the source plants of large-leaved Kudingcha (e.g., Ilex kudingcha and Ilex latifolia are discussed. It is essential to utilize emerging technologies in non-Camellia tea studies to promote the sustainable utilization of Ilex resources and the identification and development of novel compounds with potential health and clinical utility. Systems biology and “-omics” technologies will play an increasingly important role in pharmaceutical and food research on the bioactive compounds of Ilex species.

  13. The Acoustical Properties of Indonesian Hardwood Species

    Directory of Open Access Journals (Sweden)

    Tarcisius Rio Mardikanto

    2012-08-01

    Full Text Available The acoustical properties of four Indonesian tropical hardwood species were evaluated in this study. The objectives of this study were to determine acoustical parameters e.g. logarithmic decrement, sound absorption, sound velocity as well as density and wood stiffness; and to evaluate the potential of those species for acoustical purposes. Sonokeling (Dalbergia latifolia, Mahoni (Swietenia mahagony, Acacia (Acacia mangium and Manii wood (Maesopsis eminii were selected in this research. Three different cutting plane patterns of sawn timber (quarter-sawn, flat-sawn, and plain-sawn were converted into small specimens. The methods for determining acoustical properties were longitudinal vibration testing and time of flight of ultrasonic wave method. The result showed no significant difference (α=0.05 of acoustical properties in logarithmic decrement, sound absorption, and ultrasonic velocity means on quarter-sawn, flat-sawn, and plain-sawn for all wood species tested. We found that Mahoni and Sonokeling had good acoustical properties of logarithmic decrement, ultrasonic wave velocity, and ratio of wood stiffness to wood density; and is preferred for crafting musical instruments. Acacia and Manii woods are recommended for developing acoustic panels in building construction because those species possess higher sound absorption values.

  14. [Selection of winter plant species for wetlands constructed as sewage treatment systems and evaluation of their wastewater purification potentials].

    Science.gov (United States)

    Chen, Yong-hua; Wu, Xiao-fu; Chen, Ming-li; Jiang, Li-juan; Li, Ke-lin; Lei, Dian; Wang, Hai-bin

    2010-08-01

    In order to establish an evaluation system for selection of winter wetland plants possessing high wastewater purification potentials in subtropics areas, designed sewage treatment experiments were carried out by introducing into the constructed wetlands 25 species of winter wetland plants. Cluster analysis was performed by including harmful environment-resistant enzyme and substrate enzyme activities into the commonly applied plant screening and assessment indexes system. The obtained results indicated that there were significant differences among the tested winter plants in their root length and vigor, leaf malonaldehyde (MDA), biomass, average nitrogen and phosphorus concentration and uptake, and urease and phosphoric acid enzyme activities in the root areas. Based on the established evaluation system, the tested plants were clustered into 3 groups. The plants in the 1st group possessing high purification potentials are Oenanthe javanica, Brassicacapestris, Juncus effusu, Saxifragaceae, Iris pseudoacorus, Osmanthus fragrans and Iris ensata; those in the 2nd group possessing moderate purification potentials are Brassica oleracea var acephala, Calendula officinalis, Aucuba japonica, Ligustrum lucidu, Beta vulgaris, Rhododendron simsii and Ilex latifolia; and those in the 3rd group with low purification potentials are Brassica oleracea var acephala, Calistephus chinensis, Rosa chinensis, Antirrhinums, Liriope palatyphylla, Zephyranthes candida, Fatshedera lizei, Petunia hybrida, Ilex quihoui, Dianthus caryophyllus and Loropetalum chinensis.

  15. Evaluation of an innovative approach based on prototype engineered wetland to control and manage boron (B) mine effluent pollution.

    Science.gov (United States)

    Türker, Onur Can; Türe, Cengiz; Böcük, Harun; Yakar, Anıl; Chen, Yi

    2016-10-01

    A major environmental problem associated with boron (B) mining in many parts of the world is B pollution, which can become a point source of B mine effluent pollution to aquatic habitats. In this study, a cost-effective, environment-friendly, and sustainable prototype engineered wetland was evaluated and tested to prevent B mine effluent from spilling into adjoining waterways in the largest B reserve in the world. According to the results, average B concentrations in mine effluent significantly decreased from 17.5 to 5.7 mg l(-1) after passing through the prototype with a hydraulic retention time of 14 days. The results of the present experiment, in which different doses of B had been introduced into the prototype, also demonstrated that Typha latifolia (selected as donor species in the prototype) showed a good resistance to alterations against B mine effluent loading rates. Moreover, we found that soil enzymes activities gradually decreased with increasing B dosages during the experiment. Boron mass balance model further showed that 60 % of total B was stored in the filtration media, and only 7 % of B was removed by plant uptake. Consequently, we suggested that application of the prototype in the vicinity of mining site may potentially become an innovative model and integral part of the overall landscape plan of B mine reserve areas worldwide. Graphical Abstract ᅟ. PMID:27364490

  16. Distribution of seed plants with respect to tide levels and water salinity in the natural tidal marshes of the northern San Francisco Bay Estuary, California

    Science.gov (United States)

    Atwater, Brian F.; Hedel, Charles W.

    1976-01-01

    Shoaling of subtidal and intertidal mud flats has permitted tidal marshes to spread across large marginal areas of the San Francisco Bay estuary during the past several thousand years. By 1850 A.D. the tidal marshes of the estuary, including those of the Sacramento - San Joaquin Delta, covered an area nearly twice as large as the area of open water. Nearly 95 percent of these marshes have been diked or filled during the past 125 years. Species distributions along leveled transects at six tidal marshes indicate that elevation and water salinity are the principal ecological factors that-control the distribution of seed plants in the remaining natural tidal marshes of the northern San Francisco Bay estuary. Marsh surfaces situated near mean tide level are populated by robust monocotyledons (e.g., Spartina foliosa, Scirpus californicus), whereas surfaces situated near high-tide levels support dicotyledons and a few small monocotyledonous species (e.g., Salicornia virginica, Distichlis spicata). Marshes near the seaward end of the estuary are typically occupied by 10-15 salt-tolerant species (e.g., Spartina foliosa, Salicornia virginica), whereas marshes at the riverward end of the estuary are inhabited by as many as 30 species, most of which are known to tolerate moderate or small amounts of salt (e.g., Scirpus spp., Phragmites communis, Typha latifolia).

  17. Vegetation changes and partitioning of selenium in 4-year-old constructed wetlands treating agricultural drainage.

    Science.gov (United States)

    Lin, Z Q; Terry, N; Gao, S; Mohamed, S; Ye, Z H

    2010-03-01

    The knowledge of selenium (Se) partitioning in treatment wetlands and wetland vegetation management are essential for long-term effective operation of constructed wetlands treating Se-laden agricultural tile-drainage in central California. In this field study, samples from different compartments of treatment wetlands were collected and the vegetation change in each wetland cell was examined four years after the wetland's inception. The results showed that saltgrass (Distichlis spicata) and rabbitfoot grass (Polypogon monspeliensis) were less competitive than cattail (Typha latifolia) and saltmarsh bulrush (Scirpus robustus). Over 90% of the wetland cell originally vegetated with saltgrass or rabbitfoot grass was occupied by invasive plants--i.e., when invasive species were not controlled in the wetlands. More Se was likely found in sediments from vegetated regions, compared to the unvegetated areas of the wetland cell. Particularly, rhizosphere sediments accumulated about 4-fold more Se than non-rhizosphere sediments. Among the total Se retained in the wetland 90% of the total Se was partitioned in the top 10-cm layer of sediment. The Se accumulation in plant materials accounted for about 2% of the total Se mass retained in each wetland cell. This field study demonstrated that wetland plants play significant roles in the treatment of Se-laden agricultural drainage.

  18. Flora of the Mediterranean Rivers in Bulgaria

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    Yordanka G. Hristeva

    2015-06-01

    Full Text Available Species composition and distribution of aquatic bryophytes and vascular plants assemblages in Mediteranean Rivers in Bulgaria are presented in this work. Aquatic macrophytes were studied at thirteen rivers in South Bulgaria during 2014, together with abiotic factors (flow velocity, shading, and substrate type, mean depth and altitude. In total, 73 species were registered, of them 13 bryophytes and 60 vascular plants were identified. Aquatic bryophytes included 10 mosses and 3 liverworts. The recorded bryophytes species refer to 7 families and 12 genera. The most frequently distributed species was Leptodictyum riparium (Hedw. Warnst., followed by Cratoneuron filicinum (Hedw. Spruce and Platyhypnidium riparioides (Hedw. Dixon, Brachythecium rivulare Schimp. and Hygroamblystegium tenax (Hedw. Jenn. The recorded 60 species of vascular plants refer to 25 families and 43 genera. The most common hydrophyte species was Lemna minor L., followed by Ranunculus trichophyllus Chaix, Myriophyllum spicatum L. and Potamogeton nodosus Poir. The most abundant species from the group of helophytes and amphiphytes was Mentha aquatica L., followed by Agrostis stolonifera L. Mentha spicata L., Berula erecta (Huds. Coville, Juncus effusus L., Lycopus europaeus L., Lythrum salicaria L., Phalaris arundinacea L., Ranunculus repens L., Sparganium erectum L., Typha latifolia L., and Veronica anagalis-aquatica L. The majority of studied rivers sites were sunny, with moderate velocity, stony bottom, average depth up to 0.3 m and altitude between 100 and 500 m a.s.l.

  19. Revegetation dynamics after 15 years of rewetting in two extracted peatlands in Sweden

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    S.A. Kozlov

    2016-02-01

    Full Text Available The restoration of extracted peatlands poses challenges with regard to their recolonisation by vegetation. The most significant problems are unstable water levels, destroyed propagule bank, and temperature fluctuations on bare peat surfaces. Rewetting is considered necessary for the re-establishment of peat-forming vegetation. Revegetation was investigated in a long-term field study at two rewetted extracted peatland sites in south-central Sweden, namely Västkärr (originally a lagg fen and Porla (originally a bog. Both sites were expanses of bare peat before rewetting. Rewetting procedures were applied in 1999 and strongly promoted plant colonisation. At Västkärr, colonisation started in the first year after rewetting, mostly by species that were not found during repeat vegetation surveys 15 years later. By 2014, Västkärr was a shallow lake surrounded by mesotrophic and eutrophic vegetation with species such as Carex rostrata, Lemna minor, Typha latifolia, Phalaris arundinacea and Phragmites australis. Revegetation of the Porla site was slower and started with Eriophorum vaginatum and Polytrichum spp. Sphagnum mosses appeared after six years and had established quite well after 13 years. A residual Sphagnum peat layer, inflowing surface water and groundwater provided spatially variable nutrient conditions. Sphagnum species and E. vaginatum established in nutrient-poor areas, while C. rostrata, P. australis and Eriophorum angustifolium colonised more nutrient-rich locations.

  20. Herbivory in a Mediterranean forest: browsing impact and plant compensation

    Science.gov (United States)

    Focardi, Stefano; Tinelli, Aleandro

    2005-11-01

    The compensatory response of plants to defoliation is likely to have important effects on plant-ungulate equilibria in forested ecosystems. We investigated the responses of six species of Mediterranean bushes to defoliation by wild ungulates, comparing an index of browsing impact with the productivity of plants in both open and exclusion plots. The data revealed a great diversity of plant responses to herbivory: Rubus ulmifolius was able to over-compensate and replace the lost tissues. Phillyrea latifolia exhibited a similar, albeit less evident, pattern, while Cistus salvifolius was severely damaged by browsing. Other species, such as Quercus ilex, Juncus acutus and Erica arborea, were not attacked to a large extent and suffered little or no harm. The results strongly suggest that Mediterranean ecosystems may tolerate large stocking rates of ungulates. However, the reduction of plant biomass due to browsing was very different in the six studied species, suggesting that when herbivory becomes severe the structure of the ecosystem will change with the more tolerant plants becoming more abundant. We can apply these results to improve management and conservation of relict coastal forests in the Mediterranean basin which are usually of small size and where decision-makers have to compromise between the conservation of plants and that of large mammals.

  1. Facing drought in a Mediterranean post-fire community: tissue water relations in species with different life traits

    Science.gov (United States)

    Borghetti, Marco; Magnani, F.; Fabrizio, A.; Saracino, A.

    2004-03-01

    Bulk shoot water potential, the osmotic component and the bulk modulus of elasticity were measured throughout one growing season in four species co-occurring in a post-fire Mediterranean community in southern Italy: Pinus halepensis, Phillyrea latifolia, Cistus salvifolius and Rosmarinus officinalis. A severe drought occurred throughout the measurement period. Large seasonal fluctuations have been observed for both predawn and afternoon water potential in all species. Although minimum values down to -4 MPa have been measured, plant water potential always recovered to less negative values after drought. Daily amplitude of water potential decreased with increasing plant water stress in all species. In Cistus and Rosmarinus less ability for short-term control of plant water status has been assessed. Osmotic potential at full turgor did not display clear seasonal patterns, with no consistent ranking of species by their osmotic values. In most cases, no osmotic adjustment (lowering of osmotic potentials) and no change in tissue elastic properties were observed in response to increasing summer drought and intensity of water stress.

  2. Effect of two successive wildfires in Pinus halepensis stands of central Greece

    Directory of Open Access Journals (Sweden)

    Y. Spanos

    2008-05-01

    Full Text Available We estimated differences, five years after a wildfire, in soil and vegetation between Pinus halepensis stands that were once burnt (1998 and stands that were burnt twice in a short time interval (1995 and 1998, in the area of Penteli, central Greece. The parameters monitored were the physical and chemical attributes of upper soil layer and the vegetation composition, density and height. The results showed that five years after the wildfire, soil pH did not differ between areas burnt once and twice, while the organic matter was higher in the once-burnt areas. The vegetation composition was similar in the two areas and the dominant species were those pre-existing the fire. On the contrary, vegetation density was considerably lower in the twice-burnt areas. The height of woody species oscillated in the same levels in the two areas. The plant community was composed mainly by resprouting species, like Quercus coccifera, Pistacia lentiscus, or Phillyrea latifolia, and less individuals from seed-regenerated species, like Pinus halepensis, Cistus monspeliensis, and C. creticus. It is suggested that the recurrence of wildfire affected negatively the ecosystem attributes, and contributed to the increased risk of degradation.

  3. Plants and phytochemicals for peptic ulcer: An overview

    Directory of Open Access Journals (Sweden)

    Saikat Sen

    2009-01-01

    Full Text Available World is rich in medical lore. Plants are the basis of life on earth and are central to people′s livelihoods. The use of plants in religious ceremonies as well as for magic and medicinal purposes is very commonplace and widespread. Plants and phyto-constituents are better choice to treat diseases than the allopathic drugs. Most of the drugs used in primitive medicine were originated from plants and are the earliest and principal natural source of medicines. The drugs from plants are fairly innocuous and relatively free from toxic effects. The nature has provided us various medicinal plants which became the storehouse of remedies to cure all ailments of mankind. In modern era many plant-derived compounds have been used as drugs, either in their original or semi-synthetic form. Peptic ulcer is widespread and common health problem now a day. Generation of free radicals, decrease in mucosal defensive factor or increase in mucosal injurious factor causes peptic ulcer. In this review attempts have been made to know about some plants and their constituents which may be used in treatment or prevention of peptic ulcer. Various plants like Anogeissus latifolia, Alchornea castaneaefolia, Utleria salicifolia, Solanum nigrum, Ocimum sanctum, Asparagus racemosus, Scoparia dulcis, Byrsonima crassa etc. and their phyto-constituents proved active in antiulcer therapy.

  4. Long-term ecological behaviour of abandoned uranium mill tailings. 1

    International Nuclear Information System (INIS)

    Inactive uranium mill tailings were surveyed in the Province of Ontario to describe their surface characteristics, identify naturally invading biota, and determine essential chemical and physical parameters associated with the tailings. Inactive tailings sites can have wet areas, tailings completely covered with water, and dry areas. In the wet areas of most sites, wetland vegetation stands were found which were dominated by species of cattails (Typhaceae), along with some species of rushes (Juncaceae) and sedges (Cyperceae). Dry areas of the tailings exhibited a variety of surface features which are often a reflection of different amelioration efforts. Most of the indigenous species of vascular plants identified on dry areas of the tailings occurred only sporadically. Invading plants found on most sites were the tree species, trembling aspen (Populus tremuloides) and paper birch (Betula papyrifera). Elemental concentration and some physical characteristics of the tailings collected from a depth of 0-20 cm were determined. Uptake of heavy metals and radionuclides were evaluated in trees found in the dry areas and in cattails (Typha latifolia) in the wetland areas. Water bodies on tailings and surface water leaving the tailings, before and after treatment, were characterized in this survey. Aquatic bryophytes have invaded some water bodies on the tailings, and acid tolerant algae were evident in most of the water associated with the tailings. Ecological processes occurring on inactive uranium mill tailings which were identified in this survey are essential in evaluating the long-term fate of these waste sites

  5. Seedling growth and survival of selected wild edible fruit species of the Sikkim Himalaya, India

    Science.gov (United States)

    Sundriyal, Manju; Sundriyal, R. C.

    2005-07-01

    In the Sikkim Himalaya, an enormous variety of wild growing plants are exploited at large scale for collection of their edible parts, of which six most prominently utilized fruit species (viz., Baccaurea sapida, Diploknema butyracea, Elaeagnus latifolia, Eriolobus indica, Machilus edulis and Spondias axillaris) were investigated. The growth of nursery raised seedlings was measured at 3 month intervals until two years old in terms of absolute growth rate (AGR), relative growth rate (RGR), net assimilation rate (NAR), leaf area ratio (LAR), leaf weight ratio (LWR), stem weight ratio (SWR), root weight ratio (RWR) and root-shoot ratio (RSR). Spondias axillaris and Machilus edulis had the maximum AGR, RGR, LAR and SWR among all species. LWR was highest for B. sapida. RGR, LAR and LWR declined with the age of seedlings. RGR was negatively correlated with NAR, SWR, RWR and RSR, though it showed a positive relationship with LAR. For all species, seedlings attained significant sizes after one year of age, and showed reasonable survival after transplantation into the farmers' fields. It is expected that information on the growth behaviour of these species would be useful while they are adopted into agroforestry systems. It is suggested that these species should be multiplied at large scale and distributed to the local inhabitants to reduce pressure on them in natural stands as well as provide economic benefit to the subsistence farmers.

  6. Analysis of sterol and other components present in unsaponifiable matters of mahua, sal and mango kernel oil.

    Science.gov (United States)

    Dhara, Rupali; Bhattacharyya, Dipak K; Ghosh, Mahua

    2010-01-01

    The amount and characterization of phytosterol and other minor components present in three Indian minor seed oils, mahua (Madhuca latifolia), sal (Shorea robusta) and mango kernel (Mangifera indica), have been done. Theses oils have shown commercial importance as cocoa-butter substitutes because of their high symmetrical triglycerides content. The conventional thin layer chromatography (TLC), gas chromatography (GC) & gas chromatography-mass spectroscopy (GC-MS) techniques were used to characterize the components and the high performance thin layer chromatography (HPTLC) technique was used to quantify the each group of components. The experimental data showed that the all the three oils are rich in sterol content and among all the sterols, beta-sitosterol occupies the highest amount. Sal oil contains appreciable amount of cardenolides, gitoxigenin. Tocopherol is present only in mahua oil and oleyl alcohol is present in mango kernel oil. Hydrocarbon, squalene, is present in all the three oils. The characterization of these minor components will help to detect the presence of the particular oil in specific formulations and to assess its stability as well as nutritional quality of the specific oil. PMID:20299763

  7. Toxic compounds in honey.

    Science.gov (United States)

    Islam, Md Nazmul; Khalil, Md Ibrahim; Islam, Md Asiful; Gan, Siew Hua

    2014-07-01

    There is a wealth of information about the nutritional and medicinal properties of honey. However, honey may contain compounds that may lead to toxicity. A compound not naturally present in honey, named 5-hydroxymethylfurfural (HMF), may be formed during the heating or preservation processes of honey. HMF has gained much interest, as it is commonly detected in honey samples, especially samples that have been stored for a long time. HMF is a compound that may be mutagenic, carcinogenic and cytotoxic. It has also been reported that honey can be contaminated with heavy metals such as lead, arsenic, mercury and cadmium. Honey produced from the nectar of Rhododendron ponticum contains alkaloids that can be poisonous to humans, while honey collected from Andromeda flowers contains grayanotoxins, which can cause paralysis of limbs in humans and eventually leads to death. In addition, Melicope ternata and Coriaria arborea from New Zealand produce toxic honey that can be fatal. There are reports that honey is not safe to be consumed when it is collected from Datura plants (from Mexico and Hungary), belladonna flowers and Hyoscamus niger plants (from Hungary), Serjania lethalis (from Brazil), Gelsemium sempervirens (from the American Southwest), Kalmia latifolia, Tripetalia paniculata and Ledum palustre. Although the symptoms of poisoning due to honey consumption may differ depending on the source of toxins, most common symptoms generally include dizziness, nausea, vomiting, convulsions, headache, palpitations or even death. It has been suggested that honey should not be considered a completely safe food.

  8. Rootstocks for 'Tahiti' lime

    Directory of Open Access Journals (Sweden)

    Stenzel Neusa Maria Colauto

    2004-01-01

    Full Text Available The 'Tahiti' lime (Citrus latifolia Tanaka is an important commercial citrus cultivar in Brazil. 'Rangpur' lime has being used as its main rootstock, but it is susceptible to root rot caused by Phytophthora, reducing tree longevity. An experiment was set up in a randomized block design, with three trees per plot of each rootstock and four replicates, and run for 12 years, aiming to compare the performance of 'IAC-5 Tahiti' lime, budded on 'Rangpur' lime (Citrus limonia Osb.; 'C-13' citrange (Citrus sinensis (L. Osb. × Poncirus trifoliata (L. Raf.; 'African' rough lemon (Citrus jambhiri Lush.; 'Volkamer' lemon (Citrus volkameriana Ten. & Pasq.; trifoliate orange (Poncirus trifoliata (L. Raf.; 'Sunki' mandarin (Citrus sunki Hort. ex Tan. and 'Cleopatra' mandarin (Citrus reshni Hort. ex Tan.. Eleven years after the establishment of the orchard, trees with the greatest canopy development were budded on 'C-13' citrange and 'African' rough lemon, and both differed significantly from trees budded on trifoliate orange, 'Sunki' and 'Cleopatra' mandarins, which presented the smallest canopy development. Trees budded on 'Rangpur' lime and 'C-13' citrange had the highest cumulative yields, and were different from trees budded on trifoliate orange, 'Cleopatra' and 'Sunki' mandarins. There was no rootstock effect on mean fruit weight and on the total soluble solid/acid ratio in the juice. The 'Rangpur' lime and the 'Cleopatra' mandarin rootstocks reduced longevity of plants.

  9. Ecological Control Techniques of Nitrogen and Phosphorus Non-Point Source Pollution for Hill Region Paddy Field%丘陵区稻田氮磷面源污染生态治理技术试验研究

    Institute of Scientific and Technical Information of China (English)

    朱海洋; 郑世宗; 徐海波

    2009-01-01

    This paper systematically analyses effects of different crops (Zizania latifolia、lotus、chufa)and proportions(1:5、1:10、1:20) to control nitrogen and phosphorus non-point source pollution from hill region paddy field on field experiment. The suitable pattern of ecological control techniques of nitrogen and phosphorus non-point source pollution is presented,namely to construct 1:20 proportion Zizania latifolia wetland system.%针对丘陵区稻田排放的氮磷面源污染,在田间试验基础上,系统地分析了不同水生经济作物(茭白、莲藕、荸荠)、不同湿地配置比例(1:5、1:10、1:20)对其的净化效果.综合2年的试验结果,针对丘陵区稻田氮磷面源污染,研究提出了适宜的生态治理技术模式,即构建1:20配置比例的茭白湿地系统.

  10. Anti-Inflammatory Properties and Chemical Characterization of the Essential Oils of Four Citrus Species.

    Directory of Open Access Journals (Sweden)

    Jorge Luis Amorim

    Full Text Available Citrus fruits have potential health-promoting properties and their essential oils have long been used in several applications. Due to biological effects described to some citrus species in this study our objectives were to analyze and compare the phytochemical composition and evaluate the anti-inflammatory effect of essential oils (EO obtained from four different Citrus species. Mice were treated with EO obtained from C. limon, C. latifolia, C. aurantifolia or C. limonia (10 to 100 mg/kg, p.o. and their anti-inflammatory effects were evaluated in chemical induced inflammation (formalin-induced licking response and carrageenan-induced inflammation in the subcutaneous air pouch model. A possible antinociceptive effect was evaluated in the hot plate model. Phytochemical analyses indicated the presence of geranial, limonene, γ-terpinene and others. EOs from C. limon, C. aurantifolia and C. limonia exhibited anti-inflammatory effects by reducing cell migration, cytokine production and protein extravasation induced by carrageenan. These effects were also obtained with similar amounts of pure limonene. It was also observed that C. aurantifolia induced myelotoxicity in mice. Anti-inflammatory effect of C. limon and C. limonia is probably due to their large quantities of limonene, while the myelotoxicity observed with C. aurantifolia is most likely due to the high concentration of citral. Our results indicate that these EOs from C. limon, C. aurantifolia and C. limonia have a significant anti-inflammatory effect; however, care should be taken with C. aurantifolia.

  11. Anti-Inflammatory Properties and Chemical Characterization of the Essential Oils of Four Citrus Species.

    Science.gov (United States)

    Amorim, Jorge Luis; Simas, Daniel Luiz Reis; Pinheiro, Mariana Martins Gomes; Moreno, Daniela Sales Alviano; Alviano, Celuta Sales; da Silva, Antonio Jorge Ribeiro; Fernandes, Patricia Dias

    2016-01-01

    Citrus fruits have potential health-promoting properties and their essential oils have long been used in several applications. Due to biological effects described to some citrus species in this study our objectives were to analyze and compare the phytochemical composition and evaluate the anti-inflammatory effect of essential oils (EO) obtained from four different Citrus species. Mice were treated with EO obtained from C. limon, C. latifolia, C. aurantifolia or C. limonia (10 to 100 mg/kg, p.o.) and their anti-inflammatory effects were evaluated in chemical induced inflammation (formalin-induced licking response) and carrageenan-induced inflammation in the subcutaneous air pouch model. A possible antinociceptive effect was evaluated in the hot plate model. Phytochemical analyses indicated the presence of geranial, limonene, γ-terpinene and others. EOs from C. limon, C. aurantifolia and C. limonia exhibited anti-inflammatory effects by reducing cell migration, cytokine production and protein extravasation induced by carrageenan. These effects were also obtained with similar amounts of pure limonene. It was also observed that C. aurantifolia induced myelotoxicity in mice. Anti-inflammatory effect of C. limon and C. limonia is probably due to their large quantities of limonene, while the myelotoxicity observed with C. aurantifolia is most likely due to the high concentration of citral. Our results indicate that these EOs from C. limon, C. aurantifolia and C. limonia have a significant anti-inflammatory effect; however, care should be taken with C. aurantifolia.

  12. Hyperspectral reflectance response of freshwater macrophytes to salinity in a brackish subtropical marsh.

    Science.gov (United States)

    Tilley, David R; Ahmed, Muneer; Son, Ji Ho; Badrinarayanan, Harish

    2007-01-01

    Coastal freshwater wetlands are threatened by increased salinity due to relative sea level rise and reduced freshwater inputs. Remote radiometric measurement of freshwater marsh canopies to detect small shifts in water column salinity would be useful for assessing salinity encroachment. We measured leaf hyperspectral (300-1100 nm) reflectance of freshwater macrophytes (cattail, Typha latifolia and sea oxeye, Borrichia frutescens) in a field study in a subtropical brackish (2.5-4.5 parts per thousand salinity, per thousand) marsh to determine salinity effects on visible and near-infrared spectral band reflectance and to identify reflectance indices sensitive to small (1 per thousand) changes in wetland salinity. For sea oxeye, floating-position water band index [fWBI = R(900)/minimum(R(930) - R(980)), where R(lambda) = reflectance at band lambda], normalized difference vegetation index [NDVI = (R(774) - R(681))/(R(774) + R(681))], and a proposed wetland salinity reflectance ratio (WSRR = R(990)/R(933)) were sensitive to salinity with R2 of 40, 35, and 65%, respectively (p wetland salinity, which would help with monitoring salinity effects on coastal wetlands. PMID:17412913

  13. Treatment of swine wastewater in marsh-pond-marsh constructed wetlands.

    Science.gov (United States)

    Reddy, G B; Hunt, P G; Phillips, R; Stone, K; Grubbs, A

    2001-01-01

    Swine waste is commonly treated in the USA by flushing into an anaerobic lagoon and subsequently applying to land. This natural system type of application has been part of agricultural practice for many years. However, it is currently under scrutiny by regulators. An alternate natural system technology to treat swine wastewater may be constructed wetland. For this study we used four wetland cells (11 m width x 40 m length) with a marsh-pond-marsh design. The marsh sections were planted to cattail (Typha latifolia, L.) and bulrushes (Scirpus americanus). Two cells were loaded with 16 kg N ha(-1) day(-1) with a detention of 21 days. They removed 51% of the added N. Two additional cells were loaded with 32 kg ha(-1) day(-1) with 10.5 days detention. These cells removed only 37% of the added N. However, treatment operations included cold months in which treatment was much less efficient. Removal of N was moderately correlated with the temperature. During the warmer periods removal efficiencies were more consistent with the high removal rates reported for continuous marsh systems--often > than 70%. Phosphorus removal ranged from 30 to 45%. Aquatic macrophytes (plants and floating) assimilated about 320 and 35 kg ha(-1), respectively of N and P. PMID:11804147

  14. The potential role of cattail-reinforced clay plaster in sustainable building

    Directory of Open Access Journals (Sweden)

    G. Georgiev

    2014-06-01

    Full Text Available Sustainable development is a key goal in town and country planning, as well as in the building industry. The main aims are to avoid inefficient land use, to improve the energy efficiency of buildings and, thus, to move towards meeting the challenges of climate change. In this article we consider how the use of a traditional low-energy building material, namely clay, might contribute. Recent research has identified a promising connection between the reinforcement of clay for internal wall plastering with fibres from the wetland plant Typha latifolia (cattail and the positive environmental effects of cultivating this species. If large quantities of Typha fibres were to be used in building, the need for cultivation of the plant would increase and create new possibilities for the renaturalisation of polluted or/and degraded peatlands. We explore the topic first on the basis of literature, considering the suitability of Typha for this application and possibilities for its sustainable cultivation, as well as implications for the life cycle analyses of buildings in which it is used. We then report (qualitatively the results of testing different combinations of clay with natural plant (straw and cattail fibres for their suitability as a universal plaster, which demonstrate clearly the superior properties of Typha fibres as a reinforcement material for clay plaster mortars.

  15. Phytoremediation of imazalil and tebuconazole by four emergent wetland plant species in hydroponic medium.

    Science.gov (United States)

    Lv, Tao; Zhang, Yang; Casas, Mònica E; Carvalho, Pedro N; Arias, Carlos A; Bester, Kai; Brix, Hans

    2016-04-01

    Pollution from pesticide residues in aquatic environments is of increasing concern. Imazalil and tebuconazole, two commonly used systemic pesticides, are water contaminants that can be removed by constructed wetlands. However, the phytoremediation capability of emergent wetland plants for imazalil and tebuconazole, especially the removal mechanisms involved, is poorly understood. This study compared the removal of both pesticides by four commonly used wetland plants, Typha latifolia, Phragmites australis, Iris pseudacorus and Juncus effusus, and aimed to understand the removal mechanisms involved. The plants were individually exposed to an initial concentration of 10 mg/L in hydroponic solution. At the end of the 24-day study period, the tebuconazole removal efficiencies were relatively lower (25%-41%) than those for imazalil (46%-96%) for all plant species studied. The removal of imazalil and tebuconazole fit a first-order kinetics model, with the exception of tebuconazole removal in solutions with I. pseudacorus. Changes in the enantiomeric fraction for imazalil and tebuconazole were detected in plant tissue but not in the hydroponic solutions; thus, the translocation and degradation processes were enantioselective in the plants. At the end of the study period, the accumulation of imazalil and tebuconazole in plant tissue was relatively low and constituted 2.8-14.4% of the total spiked pesticide in each vessel. Therefore, the studied plants were able to not only take up the pesticides but also metabolise them. PMID:26841287

  16. Impact of a Mountain Pine Beetle Outbreak on Young Lodgepole Pine Stands in Central British Columbia

    Directory of Open Access Journals (Sweden)

    Amalesh Dhar

    2015-09-01

    Full Text Available The current mountain pine beetle (MPB (Dendroctonous ponderosae Hopkins epidemic has severely affected pine forests of Western Canada and killed millions of hectares of lodgepole pine (Pinus contorta Dougl. ex Loud. var. latifolia Engelm. forest. Generally, MPB attack larger and older (diameter > 20 cm or >60 years of age trees, but the current epidemic extends this limit with attacks on even younger and smaller trees. The study’s aim was to investigate the extent of MPB attack in young pine stands and its possible impact on stand dynamics. Although MPB attacks were observed in trees as small as 7.5 cm diameter at breast height (DBH and as young as 13 years old, the degree of MPB attack (percent stems ha−1 increased with increasing tree diameter and age class (13–20, 21–40, 41–60, and 61–80 years old (6.4%, 49.4%, 62.6%, and 69.5% attack, respectively, by age class which is greater than that reported from previous epidemics for stands of this age. The mean density of surviving residual structure varied widely among age classes and ecological subzones. Depending on age class, 65% to 77% of the attacked stands could contribute to mid-term timber supply. The surviving residual structure of young stands offers an opportunity to mitigate the effects of MPB-attack on future timber supply, increase age class diversity, and enhance ecological resilience in younger stands.

  17. Lead and zinc accumulation and tolerance in populations of six wetland plants

    Energy Technology Data Exchange (ETDEWEB)

    Deng, H. [Biology Department and Croucher Institute for Environmental Sciences, Hong Kong Baptist University, Kowloon, Hong Kong (China); Department of Environmental Science and Technology, East China Normal University, Shanghai (China); Ye, Z.H. [Biology Department and Croucher Institute for Environmental Sciences, Hong Kong Baptist University, Kowloon, Hong Kong (China); School of Life Sciences, Zhongshan (Sun Yat-sen) University, Guangzhou 510275 (China); Wong, M.H. [Biology Department and Croucher Institute for Environmental Sciences, Hong Kong Baptist University, Kowloon, Hong Kong (China)]. E-mail: mhwong@hkbu.edu.hk

    2006-05-15

    Wetland plants such as Typha latifolia and Phragmites australis have been indicated to show a lack of evolution of metal tolerance in metal-contaminated populations. The aim of the present study is to verify whether other common wetland plants such as Alternanthera philoxeroides and Beckmannia syzigachne, also possess the same characteristics. Lead and zinc tolerances in populations of six species collected from contaminated and clean sites were examined by hydroponics. In general, the contaminated populations did not show higher metal tolerance and accumulation than the controls. Similar growth responses and tolerance indices in the same metal treatment solution between contaminated and control populations suggest that metal tolerance in wetland plants are generally not further evolved by contaminated environment. The reasons may be related to the special root anatomy in wetland plants, the alleviated metal toxicity by the reduced rooting conditions and the relatively high innate metal tolerance in some species. - Populations from metal contaminated sites did not have significantly higher metal tolerance indices.

  18. The effect of temperature on forest production in Canada, Finland and Sweden. Predicted effects of a global warming on production of lodgepole pine and Scots pine in the northern boreal forest

    International Nuclear Information System (INIS)

    The aims of this study were to analyse relationships between forest production and climatic factors under different biogeoclimatic conditions and, thus, to enhance our ability to predict changes in production following temperature increases. Production in the IUFRO 70/71 provenance test series with lodgepole pine (Pinus contorta var. latifolia) was correlated to climate data from adjacent meteorological stations. Field-tests in Canada (British Columbia and the Yukon) and Scandinavia (Finland and Sweden) were evaluated about 20 years after planting. The temperature regime was strongly correlated to forest production in the northern boreal forest regions. The temperature during the growing season as a whole and the length of it seem to be more important than the maximum summer temperature. The relationship between production and temperature was weaker in Canada than in Scandinavia, and production increased generally more on poor and intermediate sites than on rich sites. According to the presented algorithms, an increase in the temperature sum from 600 to 1200 degree days, would theoretically result in an increase in site index of between 5 and 13 m for lodgepole pine, and slightly lower for Scots pine. The highest increases would occur in Scandinavia. Temperature plots show that, especially in northern Scandinavia, a higher mean temperature would prolong the growing season, and this may make short spells with above 0 deg C-temperatures during the dormant period. Together with drought during the growing season, this may increase the frequency of climate-related frost damage

  19. Study of some characteristic Mediterranean vegetation species best suited for renaturalization of terminal-phase municipal solid waste (MSW) landfills in Puglia (Southern Italy)

    Science.gov (United States)

    De Mei, Massimiliano; Di Mauro, Mariaida

    2006-07-01

    Natural recovery of worked-out or closed municipal solid waste (MSW) landfills is a current topic, but knowledge about the adaptability of Mediterranean vegetation species to such stressful conditions is still quite poor. Autochthonous plants were selected to withstand the stresses such as hot climate and drought typical of Mediterranean areas; this characteristic potentially allows the plants an easier, efficient adaptation. Our aim was to provide information in order to obtain an adequate quality of environmental renewal of a landfill and a reduced management cost while ensuring rehabilitation to an acceptable naturalistic state. The investigation lasted 3 years; some Mediterranean scrub native plant species were selected and monitored in their morphological (total and relative height, basal diameter, number of inter-nodes) and physiological (photosynthetic rate and water potential) activity. In order to test dependence on CO 2 concentration, different meteorological parameters were also monitored. Ceratonia siliqua, Phillyrea latifolia, Olea europaea and Quercus ilex showed considerable adaptability, reacting positively to every improvement in environmental conditions, particularly those of a meteorological nature. Survival and growth was satisfactory in Hedysarum coronarium, Medicago sativa, Lotus corniculatus, Rosmarinus officinalis, Myrtus communis and Viburnum tinus. Fraxinus ornus and Acer campestre suffered stress during the summer dry period and recovered quickly when atmospheric conditions improved. A drop irrigation system to ensure a satisfactory soil moisture during summer dry periods was the fundamental element for survival.

  20. Fast separation of triterpenoid saponins using supercritical fluid chromatography coupled with single quadrupole mass spectrometry.

    Science.gov (United States)

    Huang, Yang; Zhang, Tingting; Zhou, Haibo; Feng, Ying; Fan, Chunlin; Chen, Weijia; Crommen, Jacques; Jiang, Zhengjin

    2016-03-20

    Triterpenoid saponins (TSs) are the most important components of some traditional Chinese medicines (TCMs) and have exhibited valuable pharmacological properties. In this study, a rapid and efficient method was developed for the separation of kudinosides, stauntosides and ginsenosides using supercritical fluid chromatography coupled with single quadrupole mass spectrometry (SFC-MS). The separation conditions for the selected TSs were carefully optimized after the initial screening of eight stationary phases. The best compromise for all compounds in terms of chromatographic performance and MS sensitivity was obtained when water (5-10%) and formic acid (0.05%) were added to the supercritical carbon dioxide/MeOH mobile phase. Beside the composition of the mobile phase, the nature of the make-up solvent for interfacing SFC with MS was also evaluated. Compared to reversed phase liquid chromatography, the SFC approach showed higher resolution and shorter running time. The developed SFC-MS methods were successfully applied to the separation and identification of TSs present in Ilex latifolia Thunb., Panax quinquefolius L. and Panax ginseng C.A. Meyer. These results suggest that this SFC-MS approach could be employed as a useful tool for the quality assessment of natural products containing TSs as active components. PMID:26773536