Sample records for camping

  1. [Hemophilia camps. (United States)

    Juárez-Sierra, Julieta; Del Pilar Torres-Arreola, Laura; Marín-Palomares, Teresa; Dueñas-González, María Teresa; Monteros-Rincón, Martha Patricia; Osorio-Guzmán, Maricela


    We reported the experience of hemophilia camps which was accomplished with patients from hospitals of the Instituto Mexicano del Seguro Social. The aim was to prepare the families and patients regarding the disease treatment, in order to promote the self sufficiency and to know the impact of the program on the course of the disease. Surveys were applied about treatment items and personal opinions were collected. The results of the national hemophilia camp were: group of 56 patients, average 14 years, 2 % women, 51 % severe hemophilia and 43 % had hemophilic brothers. Benefits: patients increased their knowledge about earlier bleeding identification and the self-infusion method; they became aware on their responsibility in self care, timely treatment and duties at home. Hemophilia camps with patients are an option for attitude change before disease complications. Social network creation and the increase in self-sufficiency are other benefits.

  2. Lesbian camp: An unearthing. (United States)

    Nielsen, Elly-Jean


    Camp-a sensibility, a style, and a form of artistic self-expression-is an elusive concept said to be in the eye of the beholder. To refute Susan Sontag's ( 1966 ) claims that camp is apolitical and not especially homosexual, a number of recent scholarly works have been geared toward revealing camp's fundamental gayness. With the odd footnote aside, lesbian camp has been collapsed into the category of gay male camp, if not eclipsed entirely. Despite the negligible efforts made to legitimize lesbian camp, there are numerous salient cultural examples one might draw on to illustrate, typify, and substantiate a lesbian camp sensibility. I lay the ground work for this scholarly exercise by outlining various definitions and critiques of camp, and by discussing its history and application to queer theory. Then, to unveil lesbian camp, three non-mutually exclusive categories are discussed: classic, erotic, and radical. By gathering various strands of inquiry, and various textual examples (e.g., photography, artistic performances, and literary tropes), this article attempts to reach a more inclusive and nuanced understanding of lesbian camp.

  3. Recreation Summer Camps 2016 (United States)

    Montgomery County of Maryland — List of all Camps (Register here: to include Aquatics, Basketball, Soccer, Special Interest, General Sports,...

  4. Registration Summer Camp 2016

    CERN Multimedia


    Reminder: registration for the CERN Staff Association Summer Camp is now open for children from 4 to 6 years old.   More information on the website: The summer camp is open to all children. The proposed cost is 480.-CHF/week, lunch included. The camp will be open weeks 27, 28, 29 and 30, from 8:30 a.m. to 5:30 p.m. For further questions, you are welcome to contact us by email at CERN Staff Association

  5. Scrum Code Camps

    DEFF Research Database (Denmark)

    Pries-Heje, Jan; Pries-Heje, Lene; Dahlgaard, Bente


    is required. In this paper we present the design of such a new approach, the Scrum Code Camp, which can be used to assess agile team capability in a transparent and consistent way. A design science research approach is used to analyze properties of two instances of the Scrum Code Camp where seven agile teams...

  6. Orienteering in Camping. (United States)

    Larson, Elston F.

    One of the recent developments in camping is "orienteering", a program using a map and compass. Orienteering can be dovetailed into an overall camping program and used to "point up" the entire program, or it can be confined to a single simple game. The arrangement depends on the situation. The minimum age of the participants should be about 9 or…

  7. Camp's "Disneyland" Effect. (United States)

    Renville, Gary


    Describes the positive mental, physical, and social growth impacts that the camping experience had on the author, and urges camp program evaluation to plan and implement such changes. Sidebar lists steps of effective evaluation: program goals and objectives, goals of evaluation, implementation of evaluation, data analysis, and findings and…

  8. Friends' Discovery Camp (United States)

    Seymour, Seth


    This article features Friends' Discovery Camp, a program that allows children with and without autism spectrum disorder to learn and play together. In Friends' Discovery Camp, campers take part in sensory-rich experiences, ranging from hands-on activities and performing arts to science experiments and stories teaching social skills. Now in its 7th…

  9. Hitler's Death Camps. (United States)

    Wieser, Paul


    Presents a high school lesson on Hitler's death camps and the widespread policy of brutality and oppression against European Jews. Includes student objectives, instructional procedures, and a chart listing the value of used clothing taken from the Jews. (CFR)

  10. CDC Disease Detective Camp

    Centers for Disease Control (CDC) Podcasts


    The CDC Disease Detective Camp gives rising high school juniors and seniors exposure to key aspects of the CDC, including basic epidemiology, infectious and chronic disease tracking, public health law, and outbreak investigations. The camp also helps students explore careers in public health.  Created: 8/2/2010 by Centers for Disease Control and Prevention (CDC).   Date Released: 8/2/2010.

  11. Rehabilitating camp cities : community driven planning for urbanised refugee camps


    Misselwitz, Philipp


    Focussing on Palestine refugee camps in the Near East, this dissertation aims to shed light on the potential relevance of urban planning to refugee camp environments worldwide. In particular, there is a focus on the role architects and urban planners can play in facilitating participatory planning processes as well as providing guidance and expertise in the development of a spatial vision for Camp Cities. Part I - The Urbanisation of Refugee Camps as a Global Challenge The first part o...

  12. The camp model for entrepreneurship teaching

    DEFF Research Database (Denmark)

    Bager, Torben


    Artiklen omhandler brugen af camps i entrepreneurship undervising - illustreret med danske camp eksempler Udgivelsesdato: online 31.03.2010......Artiklen omhandler brugen af camps i entrepreneurship undervising - illustreret med danske camp eksempler Udgivelsesdato: online 31.03.2010...

  13. Base Camp Design Simulation Training (United States)


    The Army needs officers and noncommissioned officers with requisite base camp competencies. The Army’s Field Manual (FM) 3-34.400 defines a Base Camp...reason, we designed a 600-man base camp on VBS2TM from an AutoCAD diagram found on the Theater Construction Management System (version 3.2). Known

  14. Running Boot Camp

    CERN Document Server

    Toporek, Chuck


    When Steve Jobs jumped on stage at Macworld San Francisco 2006 and announced the new Intel-based Macs, the question wasn't if, but when someone would figure out a hack to get Windows XP running on these new "Mactels." Enter Boot Camp, a new system utility that helps you partition and install Windows XP on your Intel Mac. Boot Camp does all the heavy lifting for you. You won't need to open the Terminal and hack on system files or wave a chicken bone over your iMac to get XP running. This free program makes it easy for anyone to turn their Mac into a dual-boot Windows/OS X machine. Running Bo

  15. A Summer Camp

    Institute of Scientific and Technical Information of China (English)


    正This summer,I had some special days.I joined Dongzhou International Educational Exchange Summer Camp. First,I will tell you about our foreign teachers,they are Shrina and Rebecca. They are friendly and beautiful.They are students at Oxford University. We talked about many things:famous people,subjects in England,different jobs, our deal days,western star signs,what can we say in a restaurant and so on.

  16. Interprofessional Flight Camp. (United States)

    Alfes, Celeste M; Rowe, Amanda S


    The Dorothy Ebersbach Academic Center for Flight Nursing in Cleveland, OH, holds an annual flight camp designed for master's degree nursing students in the acute care nurse practitioner program, subspecializing in flight nursing at the Frances Payne Bolton School of Nursing at Case Western Reserve University. The weeklong interprofessional training is also open to any health care provider working in an acute care setting and focuses on critical care updates, trauma, and emergency care within the critical care transport environment. This year, 29 graduate nursing students enrolled in a master's degree program from Puerto Rico attended. Although the emergency department in Puerto Rico sees and cares for trauma patients, there is no formal trauma training program. Furthermore, the country only has 1 rotor wing air medical transport service located at the Puerto Rico Medical Center in San Juan. Flight faculty and graduate teaching assistants spent approximately 9 months planning for their participation in our 13th annual flight camp. Students from Puerto Rico were extremely pleased with the learning experiences at camp and expressed particular interest in having more training time within the helicopter flight simulator.

  17. Registration Day-Camp 2016

    CERN Multimedia

    Nursery School


    Reminder Registration for the CERN Staff Association Day-camp are open for children from 4 to 6 years old More information on the website: The day-camp is open to all children. An inscription per week is proposed, cost 480.-CHF/week, lunch included The camp will be open weeks 27, 28, 29 and 30, from 8:30 am to 5:30 pm. For further questions, thanks you for contacting us by email at

  18. Summer Camp as Therapeutic Context: The Camp Logan Program. (United States)

    McCammon, Susan; And Others

    These symposium papers describe various aspects of the Camp Logan, South Carolina, program, a therapeutic summer residential program for children, ages 8-14, who have significant behavior problems. The philosophy and advantages of the therapeutic camping model are discussed, e.g., structure during the summer, controlled though informal…

  19. Summer Camp, July 2016

    CERN Multimedia

    Staff Association


    During the month of July, the Staff Association’s Children’s Day-Care Centre and School EVEE held a summer camp for 4- to 6-year-olds. 24 children altogether joined in on the adventures. On the summer camp, the children got to “travel” to a different continent of the world every week. Day after day, they would pass through make-believe Customs upon arrival and get their passports stamped by a “customs officer”. For the first week, we went on a trip to Africa. In the spirit of the theme, the children got to do plenty of crafts and coloring, make their own little bindles and play various games. They even had the chance to visit the Museum of Ethnography in Geneva (MEG), learn to play the balafon and make musical instruments with Sterrenlab. For the second week, we set off to discover the Americas, exploring both the South and the North. Alongside different workshops (singing, dancing, storytelling, crafts), the children could enjoy several special ac...

  20. Kids Camping Takes the Challenge. (United States)

    James, Vickie L.; Hohnbaum, Claudia


    A Wisconsin Girl Scout camp integrated The Healthy Kids Challenge into its program. The camp evaluated policies related to meals, snacks, physical activities, team building, and self-esteem. Staff inservice training resulted in healthier meals on the same budget and developed ownership of the program. Campers and families had opportunities to…

  1. Encountering Child Abuse at Camp. (United States)

    Durall, John K.


    Defines child abuse, including the three categories: physical, sexual, and psychological. Presents characteristics and behaviors of each type of abuse, and long-term effects. Discusses how to handle abuse that occurs at camp, and the effects on the camp. Sidebars present abuse statistics, 15 activities that promote psychological wellness, and 8…

  2. Day Camp Manual: Administration. Book I. (United States)

    Babcock, William

    The first book in a 5-book manual on day camping focuses on summer day camp administration. The book defines day camps as organized group experiences in outdoor living on a day-by-day basis and under trained leadership. It includes a philosophy of day camping, noting benefits to the campers. The book is divided into further chapters that describe…

  3. Slave Labor Camps of the Third Reich. (United States)

    Stone, Adolf


    Describes the ground rules used by Nazi architects in choosing the sites for slave labor camps. While some, like Auschwitz, became extermination camps, others also produced armaments. One camp, Theresienstadt, became a "model" camp to show to reporters and Red Cross representatives. (CS)

  4. Comparison of work motivation in camp supervisors and camp counselors in Greek private camps

    Directory of Open Access Journals (Sweden)

    George Costa


    Full Text Available The purpose of this study was twofold. First, the study sought to better understand the work motivators that led camp supervisors and counselors to accept their job. Second, the study sought to better understand the ranking and rating of 20 work motivators from supervisors and camp counselors. Responders to the research questionnaire (n=121 were camp supervisory staff and counselors, age 15-55, working in seven private camps in Greece. Two instruments were used to collect data. The first instrument collected demographic data while the second instrument focused on ranking and rating 20 work motivators. The study suggested that Herzberg's theory (Motivator / Hygiene does not apply on a full scale. The results suggested that supervisory staff indicated good working conditions, meeting other people and carrying out personal growth are important. In addition, they don't like travel and don't consider working in a camp, as a stable job. The camp counselors want to have fun in their job and the opportunity to work with youth. They also don't consider working in a camp, as a stable job and they don't accept the responsibility in proportion to their position.

  5. Registration Day-Camp 2016

    CERN Multimedia

    Nursery School


    Registration for the CERN SA Day-camp are open for children from 4 to 6 years old From March 14 to 25 for children already enrolled in CERN SA EVE and School From April 4 to 15 for the children of CERN members of the personnel (MP) From April 18 for other children More information on the website: The day-camp is open to all children. An inscription per week is proposed, cost 480.-CHF/week, lunch included The camp will be open weeks 27, 28, 29 and 30, from 8:30 am to 5:30 pm. For further questions, thanks you for contacting us by email at

  6. Hydromania: Summer Science Camp Curriculum.

    Energy Technology Data Exchange (ETDEWEB)

    Moura, Joan


    In 1992, Bonneville Power Administration (BPA) and the US Department of Energy (DOE) began a collaborative pilot project with the Portland Parks and Recreation Community Schools Program and others to provide summer science camps to children in Grades 4--6. Camps run two weeks in duration between late June and mid-August. Sessions are five days per week, from 9 a.m. to 3 p.m. In addition to hands-on science and math curriculum, at least three field trips are incorporated into the educational learning experience. The purpose of the BPA/DOE summer camps is to make available opportunities for fun, motivating experiences in science to students who otherwise would have difficulty accessing them. This includes inner city, minority, rural and low income students. Public law 101-510, which Congress passed in 1990, authorizes DOE facilities to establish collaborative inner-city and rural partnership programs in science and math. A primary goal of the BPA summer hands on science camps is to bring affordable science camp experiences to students where they live. It uses everyday materials to engage students` minds and to give them a sense that they have succeeded through a fun hands-on learning environment.

  7. Starting a Technology Camp for Children. (United States)

    Litowitz, Len S.; Baylor, Steven C.


    Presents information for starting and maintaining a technology camp for students. Includes selecting topics, identifying participants, marketing, managing the camp, budgeting, and benefits to students and host institutions. (JOW)

  8. Eurasia Project—2007 Italian Camp Held

    Institute of Scientific and Technical Information of China (English)


    <正>From July 14 to 29,the Eurasia Project—2007 Italian Camp was held at the Castle Fusano Country Club in Rome. 52 high school students from Germany,Poland,Italy and China participated in the summer camp.

  9. Marketing Camp to Parents and Children. (United States)

    Cony, Steven R.


    An effective camp marketing strategy should address both parents' and children's concerns that influence decisions about camp. Includes strategies for developing a targeted message through print media or video that addresses these concerns and persuades families to choose camp. Stresses the importance of following up with parents and children. (LP)

  10. Extension Sustainability Camp: Design, Implementation, and Evaluation (United States)

    Brain, Roslynn; Upton, Sally; Tingey, Brett


    Sustainability Camps provide an opportunity for Extension educators to be in the forefront of sustainability outreach and to meet the growing demand for sustainability education. This article shares development, implementation, and evaluation of an Extension Sustainability Camp for youth, grades 4-6. Camp impact was measured via daily pre-and…

  11. Summer Science Camps Program (SSC). (United States)

    National Science Foundation, Washington, DC. Directorate for Education and Human Resources.

    The Summer Science Camps (SSC) Program supports residential and commuter enrichment projects for seventh through ninth grade minority students who are underrepresented in science, engineering, and mathematics. Eligible organizations include school districts, museums, colleges, universities, and nonprofit youth-centered and/or community-based…

  12. The Emotional Benefits of Camping. (United States)

    Johnson, Rebecca Cowan


    Regardless of participant background, age, or ethnic origin, camp can aid in the following key components of emotional maturity: open, positive and appropriate expression of feelings; self-acceptance; a sense of self; an awareness and acceptance of others and their feelings; the ability to develop relationships; and emotional stability. (LP)

  13. The NAO goes to camp

    NARCIS (Netherlands)

    Wigdor, N.; Fraaije, A.; Solms, L.; Greeff, J. de; Janssen, J.; Blanson Henkemans, O.A.


    ALIZ-E is a Europe-wide project focusing on long-term child-robot interaction, specifically as a means of educating diabetic children on their condition. This video showcases a recent field study at "SugarKidsClub", a camp devoted to helping 7-12 year-olds handle type-1 diabetes. A wide range of CRI

  14. Summer Camp July 2017 - Registration

    CERN Multimedia

    EVE et École


    The CERN Staff Association’s Summer Camp will be open for children from 4 to 6 years old during four weeks, from 3 to 28 July. Registration is offered on a weekly basis for 450 CHF, lunch included. This year, the various activities will revolve around the theme of the Four Elements. Registration opened on 20 March 2017 for children currently attending the EVE and School of the Association. It will be open from 3 April for children of CERN Members of Personnel, and starting from 24 April for all other children. The general conditions are available on the website of the EVE and School of CERN Staff Association: For further questions, please contact us by email at

  15. Huijia School Summer Camp Program

    Institute of Scientific and Technical Information of China (English)


    As an open and international educational institution, Beijing Huijia Private School is located in Changping, a scenic district in Beijing's northern suburb. In order to strengthen international cultural exchanges, promote the study of Chinese language and the spread of Chinese culture, and make the world know more about China, Huijia School regularly organizes various summer camps for students of different ages every year. Until now, we have already successfully received more than 1,000 students from hom...

  16. Contingency Base Camp Solid Waste Generation (United States)


    wastes gener- ated at Army base camps. The data in this report were obtained from solid waste characterization surveys of base camps in Bosnia, Kosovo ...ER D C/ CE RL T R- 13 -1 7 Contingency Base Camp Solid Waste Generation Co ns tr uc tio n En gi ne er in g R es ea rc h La bo ra to...Contingency Base Camp Solid Waste Generation Stephen D. Cosper, H. Garth Anderson, Kurt Kinnevan, and Byung J. Kim Construction Engineering Research

  17. Cis-Lunar Base Camp (United States)

    Merrill, Raymond G.; Goodliff, Kandyce E.; Mazanek, Daniel D.; Reeves, John D., Jr.


    Historically, when mounting expeditions into uncharted territories, explorers have established strategically positioned base camps to pre-position required equipment and consumables. These base camps are secure, safe positions from which expeditions can depart when conditions are favorable, at which technology and operations can be tested and validated, and facilitate timely access to more robust facilities in the event of an emergency. For human exploration missions into deep space, cis-lunar space is well suited to serve as such a base camp. The outer regions of cis-lunar space, such as the Earth-Moon Lagrange points, lie near the edge of Earth s gravity well, allowing equipment and consumables to be aggregated with easy access to deep space and to the lunar surface, as well as more distant destinations, such as near-Earth Asteroids (NEAs) and Mars and its moons. Several approaches to utilizing a cis-lunar base camp for sustainable human exploration, as well as some possible future applications are identified. The primary objective of the analysis presented in this paper is to identify options, show the macro trends, and provide information that can be used as a basis for more detailed mission development. Compared within are the high-level performance and cost of 15 preliminary cis-lunar exploration campaigns that establish the capability to conduct crewed missions of up to one year in duration, and then aggregate mass in cis-lunar space to facilitate an expedition from Cis-Lunar Base Camp. Launch vehicles, chemical propulsion stages, and electric propulsion stages are discussed and parametric sizing values are used to create architectures of in-space transportation elements that extend the existing in-space supply chain to cis-lunar space. The transportation options to cis-lunar space assessed vary in efficiency by almost 50%; from 0.16 to 0.68 kg of cargo in cis-lunar space for every kilogram of mass in Low Earth Orbit (LEO). For the 15 cases, 5-year campaign

  18. How to run a successful and educational basketball camp


    Brooke LeMar; Joe Deutsch


    Camps are a great introduction to the sport of basketball for children. Universities and colleges usually offer different types of camps, typically during the summer months. Depending on the skill and maturity level of the player, basketball camps can serve a variety of purposes. Some popular types of camps include offensive skills, shooting, team, and youth camps. Regardless of the camp that is chosen, children need to have goals set for themselves before, during, and after to enhance the be...

  19. Mental health in Palestinian camps in Lebanon

    Directory of Open Access Journals (Sweden)

    Fabio Forgione


    Full Text Available Health agencies in refugee camps face the dual challenge of, firstly,convincing both camp populations and the international communitythat mental health disorders deserve treatment as much as any otherillness – and, secondly, building enough trust to encourage people toseek that treatment.

  20. Children with Cancer: Positive Benefits of Camp. (United States)

    Winfree, Christy; Williams, Richard; Powell, Gwynn M.


    A relatively new method of helping pediatric cancer patients cope with their illness is specially designed summer camps. Camp helps children with cancer address psychological effects of the disease, bodily changes, and self-concept, and helps parents and siblings cope. Sidebars present resources and tips on incorporating children with cancer into…

  1. Why It's Good to Go to Camp (United States)

    Howell, Matthew


    In the author's fourth year of undergraduate studies at the University of Waterloo he had the opportunity to explore the benefits attained by children attending a summer camp by way of an academic literature review. The author worked with Dr. Troy Glover who has been commissioned by a group of camping associations to perform a study on the…

  2. Accounting Boot Camp for College Juniors (United States)

    Myring, Mark; Wrege, William; Van Alst, Lucinda


    We describe a day-long introduction to new accounting majors, which we call a boot camp. Boot camp it is an effort to make juniors more aware of their identity, career purposes and learning resources that are now parts of their world, much of which is not covered explicitly in the accounting curriculum. This paper provides an overview of the…

  3. Opening of a summer camp at CERN

    CERN Multimedia

    Nursery School


    The Staff Association has the pleasure to announce the opening of a summer camp in l’EVE et Ecole de l’AP du CERN. With a capacity of 40 children, aged 4 to 6 years, it will be open from July 6 to 30. Registration Summer camp 2015 Registration for the CERN SA Summer camp for children aged 4 to 6 is open 16 to 30 April 2015 More information on the website: The Summer camp is open to all children of CERN Staff. An inscription per week is proposed, cost 480.-CHF/week, lunch included. The camp will be open weeks 28, 29, 30 and 31, from 8:30 am to 5:30 pm.

  4. Generation solar case study : solar summer camp

    Energy Technology Data Exchange (ETDEWEB)



    This document presented a case study of the use of solar power at camp Tanamakoon in Ontario's Algonquin Park. It discussed camp facilities which include solar powered composting toilets and solar heated showers. Composting, recycling, and use of environmentally friendly products were also discussed. The camp also has a grid interactive solar electric system and a solar water heating system. The solar electric system provides backup power to critical loads such as safety lights and an emergency fridge and is also connected to the existing grid electricity system. Any excess energy from the solar system can be used by other kitchen appliances or, any other load anywhere in the camp. The main user of the solar heated water is a large automatic dishwasher which has as a built-in boost heater for those days when the solar heated water is insufficiently hot to sanitize dishes. It was concluded that while camp utility bills have been reduced by this investment in renewable energy technology, the primary objectives of the project were the protection of Tanamakoon's pristine Algonquin environment and the attraction and retention of clients for the camp by enhancing the camping experience. fig.

  5. Design and Development Issues for Educational Robotics Training Camps (United States)

    Ucgul, Memet; Cagiltay, Kursat


    The aim of this study is to explore critical design issues for educational robotics training camps and to describe how these factors should be implemented in the development of such camps. For this purpose, two robotics training camps were organized for elementary school students. The first camp had 30 children attendees, and the second had 22. As…

  6. 2012 USGS Lidar: Brooks Camp (AK) (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. Geological Survey (USGS) had a requirement for high resolution Lidar needed for mapping the Brooks Camp region of Katmai National Park in Alaska....

  7. ESTCP Live Site Demonstrations Former Camp Beale (United States)


    instrumentation package includes a real-time kinematic global positioning system ( RTK - GPS ) receiver for recording positional data and inertial measurement...RS232C ports. For the survey at Camp Beale, the RTK - GPS base station consisted of a Trimble R8 receiver and Trimble HPB450 external radio. CH2M...the former Camp Beale were selected by the ESTCP Program Office. Survey benchmarks for RTK - GPS base station locations had also been established by

  8. Los campings en España

    Directory of Open Access Journals (Sweden)

    Francisco Feo Parrondo


    Full Text Available Los campings son una de las variedades turísticas con más crecimiento en España en las últimas décadas: 52’76% el número de campings y 60’22% el de plazas que ofrecen entre 1980 y 2002. Aunque están presentes en todas las provincias, se ubican mayoritariamente en zonas costeras, superando en muchas localidades a los alojamientos hoteleros.

  9. Camp Sherman, Ohio: History of a World War I Training Camp (United States)


    v Preface...85 Report Documentation Page ERDC/CERL TR-15-25 v Figures... Combed and Brushed on Camp Sherman Parade Ground for Photograph,” The Camp Sherman News, 5 November 1918, 5. ERDC/CERL TR-15-25 38 Figure 32

  10. Camping in the Disciplines: Assessing the Effect of Writing Camps on Graduate Student Writers (United States)

    Busl, Gretchen; Donnelly, Kara Lee; Capdevielle, Matthew


    In the past ten years, an increasing number of universities have begun organizing writing "camps," or full-week immersion experiences, in an effort to address the increased need to support graduate student writing. Outside of anecdotes and testimonials, we have previously had very little data about these camps' success. This study,…

  11. A specialized program for children with developmental disabilities within a "typical" overnight summer camp: Camp Ramah's Tikvah Program. (United States)

    Blas, Howard I


    The Tikvah Program is an overnight camping program at Camp Ramah in New England that serves campers with a range of developmental disabilities. The program has evolved over its 37-year history and includes a camping program, vocational training program, and inclusion program. Select graduates are hired by the camp for summer employment. The Tikvah Program offers a model for serving campers with special needs within a larger "typical" summer camp. Although serving the needs of such campers offers unique challenges, the presence of such a program in a regular summer camp offers tremendous opportunities and benefits for campers with special needs and more typically developing campers.

  12. Contingency Base Camp Operations and Management: Staffing and Organization (United States)


    in a low-intensity conflict such as peacekeeping. The military devel- oped a number of enduring base camps such as Camp Bondsteel in Kosovo and and engineering support All base camps require provision of potable water, waste water collection and treatment, solid waste collection and...IBCT (TF Red Bulls). Because the BCT also had responsibility for their International Security Assistance Force (ISAF) mission outside the wire

  13. Trainer Guide: Business and Finance Managerial. Camp Administration Series. (United States)

    Farley, Elizabeth, Ed.

    Suggested ideas on conducting a managerial workshop for camp directors are offered in this trainer's guide. Workshops must be at least one full day of training (6 hours) on each topic to be counted toward the American Camping Association (ACA) Camp Director Certification Program. Suggested topics to be addressed are: (1) basic principles and…

  14. 14 CFR 91.1427 - CAMP: Manual requirements. (United States)


    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false CAMP: Manual requirements. 91.1427 Section... Operations Program Management § 91.1427 CAMP: Manual requirements. (a) Each program manager who maintains program aircraft under a CAMP must put in the operating manual the chart or description of the...

  15. Benefits of Residential and Nonresidential Youth Summer Camps. (United States)

    Ross, David M.; Driver, B. L.


    Describes survey made as part of Youth Conservation Corps evaluation. Compares personal benefits of residential camping with benefits of nonresidential camps. Concludes residential participants benefited in different ways and to greater extent than nonresidential campers. Residential camping benefits measurable at least nine months after…

  16. Camp Health Aide Manual = Manual para trabajadores de salud. (United States)

    Robinson, June Grube; And Others

    This bilingual manual serves as a textbook for migrant Camp Health Aides. Camp Health Aides are members of migrant labor camps enlisted to provide information about health and social services to migrant workers and their families. The manual is divided into 12 tabbed sections representing lessons. Teaching notes printed on contrasting paper…

  17. Summer Camp and Positive Youth Development: Program with Romanian Youth (United States)

    Feenstra, Jennifer S.


    A variety of activities are used in camps to help promote positive youth development, improving social skills and self-esteem in campers. I expanded on previous camp research in this study to address the influence camps have on trust, belief in the honesty of others, empowerment, and care for others in youth in Eastern Europe. Since 1999, New…

  18. 22 CFR 62.30 - Camp counselors. (United States)


    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Camp counselors. 62.30 Section 62.30 Foreign Relations DEPARTMENT OF STATE PUBLIC DIPLOMACY AND EXCHANGES EXCHANGE VISITOR PROGRAM Specific Program... programs promote international understanding by improving American knowledge of foreign cultures...

  19. Conduct Disorders: Are Boot Camps Effective? (United States)

    Jeter, LaVaughn V.


    Youth diagnosed with "conduct disorder" are often placed in programs using forced compliance and coercive control. One type of intervention used to treat conduct disorder is the boot camp. The basic idea is that disruptive behaviors can be corrected by strict behavioral regulation and an emphasis on skills training (Weis & Toolis 2009; Weis,…

  20. CS Radar Imaging via Adaptive CAMP

    NARCIS (Netherlands)

    Anitori, L.; Otten, M.P.G.; Hoogeboom, P.


    In this paper we present results on application of Compressive Sensing (CS) to high resolution radar imaging and pro- pose the adaptive Complex Approximate Message Passing (CAMP) algorithm for image reconstruction. CS provides a theoretical framework that guarantees, under certain assumptions, recon

  1. E. Coli: Preventing Outbreaks at Camp. (United States)

    McKinney, Mary D.


    One strain of E. coli is not usually found in foods, but has been related to consumption of undercooked ground beef. Symptoms are stomach cramps and diarrhea, and 2-7% of infections lead to hemolytic uremic syndrome, which is life threatening. Camps can prevent outbreaks by avoiding uncooked meat on overnight campouts and requiring appropriate…

  2. 36 CFR 13.1402 - Camping. (United States)


    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Camping. 13.1402 Section 13.1402 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR NATIONAL PARK SYSTEM UNITS IN ALASKA Special Regulations-Klondike Gold Rush National Historical Park §...

  3. Summer camps for children with burn injuries: a literature review. (United States)

    Maslow, Gary R; Lobato, Debra


    The first summer camps for children with burn injuries started over 25 years ago, and as of 2008, there were 60 camps worldwide. This review examines the literature on summer pediatric burn camps. The authors describe common characteristics of burn camp structure, activities, and staffing and then examine the scientific evidence regarding the effect of burn camp programs on campers and camp staff volunteers. A search of Pubmed and Psychinfo databases from 1970 to 2008 for articles related to pediatric burn summer camps identified 17 articles, of which 13 fit the inclusion criteria. Existing literature consists primarily of qualitative studies, suggesting that burn camp can decrease camper isolation, improve self-esteem, and promote coping and social skills. Studies examining volunteer staff at burn camp have consistently found that there are both personal and professional benefits. Quantitative studies of self-esteem have yielded equivocal results. No studies have examined safety or the effect of burn camp on medical or rehabilitation outcomes. For the past 25 years, pediatric summer camps for children with burn injuries have played an important rehabilitation role and provided a strong community that benefits both campers and staff. Future research using more rigorous research methods and examining a broader range of outcomes (eg, safety and medical/rehabilitation outcomes) is recommended.

  4. How to run a successful and educational basketball camp

    Directory of Open Access Journals (Sweden)

    Brooke LeMar


    Full Text Available Camps are a great introduction to the sport of basketball for children. Universities and colleges usually offer different types of camps, typically during the summer months. Depending on the skill and maturity level of the player, basketball camps can serve a variety of purposes. Some popular types of camps include offensive skills, shooting, team, and youth camps. Regardless of the camp that is chosen, children need to have goals set for themselves before, during, and after to enhance the benefits. Each camper should have the opportunity to grow and develop as a basketball player as well as an individual. Running a successful camp is not only rewarding for the coaches, but for the players and campers too, which ultimately leads to the campers choosing to come back every year.

  5. Yoga camp in Ayurvedgrams of Chhattisgarh

    Directory of Open Access Journals (Sweden)

    Raghavendra Madhu


    Full Text Available The clinical and empirical health benefits of yoga and pranayam have been reiterated through research. Yoga is being adopted as a system to alleviate the burden of noncommunicable diseases (NCDs across the globe. The Directorate of AYUSH, Government of Chhattisgarh (DoA, GoCG conducts annual 5-day-yoga camp across 146 Ayurvedgrams in the State. The present article brings out the AYUSH initiatives the State is taking toward active ageing. A total of 71,096 people participated in the 5-day-yoga camp across the State. A mean participation of 5079 people over 5 days was reported across districts. Such statewide practices need to be promoted and appraised.

  6. Flaubert et Du Camp : quelques remarques

    Directory of Open Access Journals (Sweden)

    Michel Brix


    Full Text Available Les spécialistes de Flaubert ont fait de nombreux reproches à Maxime Du Camp et ne prêtent plus guère d’attention à ce qu’il a écrit. C’est une situation très regrettable, qui prive les lecteurs d’informations nombreuses, susceptibles d’éclairer les enjeux mais aussi les impasses et les contradictions du projet esthétique de Flaubert. Quelques cas significatifs sont évoqués dans le présent article, qui se penche plus particulièrement sur la ressemblance entre un passage du livre de Du Camp Le Nil (1854 et les lignes de L’Éducation sentimentale (1869 qui rapportent l’“apparition” de Mme Arnoux.

  7. Core Concepts: Orthopedic Intern Curriculum Boot Camp. (United States)

    Seeley, Mark A; Kazarian, Erick; King, Brandon; Biermann, Janet S; Carpenter, James E; Caird, Michelle S; Irwin, Todd A


    Orthopedic surgical interns must gain a broad array of clinical skills in a short time. However, recent changes in health care have limited resident-patient exposures. With the reported success of simulation training in the surgical literature, the American Board of Orthopaedic Surgery (ABOS) and Residency Review Committee for Orthopaedic Surgery have required that surgical simulation training be a component of the intern curricula in orthopedic surgical residencies. This study examined the short-term effectiveness of an orthopedic "intern boot camp" covering 7 of 17 simulation training concept modules published by the ABOS. Eight orthopedic post-graduate year 1 (PGY-1) residents (study group) completed a structured 3-month curriculum and were compared with 7 post-graduate year 2 (PGY-2) residents (comparison group) who had just completed their orthopedic surgical internship. Seven core skills were assessed using both task-specific and global rating scales. The PGY-1 residents demonstrated a statistically significant improvement in all 7 modules with respect to their task-specific pre-test scores: sterile technique (P=.001), wound closure (P<.001), knot tying (P=.017), casting and splinting (P<.001), arthrocentesis (P=.01), basics of internal fixation (P<.001), and compartment syndrome evaluation (P=.004). After the camp, PGY-1 and -2 scores in task-specific measures were not significantly different. A 3-month simulation-based boot camp instituted early in orthopedic internship elevated a variety of clinical skills to levels exhibited by PGY-2 residents.

  8. Radioprotection of the rat parotid gland by cAMP

    Energy Technology Data Exchange (ETDEWEB)

    Sodicoff, M.; Conger, A.D.


    Most earlier studies showing a radioprotective effect by cAMP show only slight degrees of protection. The present study demonstrates a substantial protective effect (DMF, 1.63) of exogenously administered cAMP on the rat parotid gland and supports the mechanism suggested previously for protection afforded the parotid glands by the ..beta..-adrenergic agonist isoproterenol, which is known to elevate endogenous intracellular cAMP.

  9. Adventure Code Camp: Library Mobile Design in the Backcountry

    Directory of Open Access Journals (Sweden)

    David Ward


    Full Text Available This article presents a case study exploring the use of a student Coding Camp as a bottom-up mobile design process to generate library mobile apps. A code camp sources student programmer talent and ideas for designing software services and features.  This case study reviews process, outcomes, and next steps in mobile web app coding camps. It concludes by offering implications for services design beyond the local camp presented in this study. By understanding how patrons expect to integrate library services and resources into their use of mobile devices, librarians can better design the user experience for this environment.

  10. Camp Insurance 101: Understanding the Fundamentals of a Camp Insurance Program. (United States)

    Garner, Ian


    This short course on insurance for camps discusses coverage, including the various types of liability, property, and other types of coverage; the difference between direct writers, brokers, agents, and captive agents; choosing an insurance company; and checking on the financial stability of recommended carriers. Three Web sites are given for…

  11. Staphylococcus pseudintermedius for CAMP-test. (United States)

    Savini, Vincenzo; Paparella, Antonello; Serio, Annalisa; Marrollo, Roberta; Carretto, Edoardo; Fazii, Paolo


    CAMP test reliably detects Listeria monocytogenes (Lm) and Streptococcus agalactiae (group B streptococcus, GBS); it is traditionally performed streaking the tested isolate perpendicularly to Staphylococcus aureus (Sa), provided that reference Sa strains (that produce β-hemolysin) are used. In a zone of β-hemolysin activity, in fact, GBS and Lm form typical arrow-shaped hemolytic areas. While Sa production of the toxin is strain-dependent, however, that of Staphylococcus pseudintermedius (Sp), a pet-owner colonizer and an emerging human pathogen, is constitutive, then observed in all clinical isolates. Therefore, Sp may indeed represent a valid alternative to perform the assay.

  12. How to Respond to Problem Behavior in Camp. (United States)

    Rotman, Charles B.


    Deals with types of behavior that cannot be allowed among staff or campers in the camp community--use of drugs or alcohol, personal abuse, crime, violence, and sexual activity. Urges camp directors to develop a written policy spelling out standards, expectations, and sanctions. Suggests staff orientation strategies. (JHZ)

  13. Socialization of Adolescents: Cultural Practices in Children's Summer Camp (United States)

    Demakova, Irina D.; Valeeva, Roza A.; Shipova, Alina V.


    The article describes the relevant aspects of the adolescents' cultural practices in children's summer camp, taking into account their specific characteristics. The summer camp is considered as an educational formation and holistic socio-pedagogical body, designed to create conditions for the development of the person. The criteria for inclusion…

  14. Students Become Scientists at Science Skills Boot Camp | Poster (United States)

    At the 2016 Science Skills Boot Camp (SSBC), a one-day training program designed for NIH summer interns with little or no prior research experience, students gathered to learn about basic research and laboratory skills. The boot camp provided a unique opportunity for interns to expand their knowledge of simple bench techniques, scientific papers, and ways to communicate their research.

  15. EduCamp Colombia: Social Networked Learning for Teacher Training (United States)

    Fonseca, Diego Ernesto Leal


    This paper describes a learning experience called EduCamp, which was launched by the Ministry of Education of Colombia in 2007, based on emerging concepts such as e-Learning 2.0, connectivism, and personal learning environments. An EduCamp proposes an unstructured collective learning experience, which intends to make palpable the possibilities of…

  16. College and University Summer Camps: Creative Alternatives to Day Care. (United States)

    Wever, Matthew F.


    The successful summer day camp program of Saint Louis University (Missouri) is described. Program rationale, philosophy, objectives, structure, staffing, activities, and benefits to the university community are discussed. A majority of the children served by the camp are the children, ages 6 to 12, of university employees. (MSE)

  17. Specialized Summer Camps: Provide Benefits for Children and Families Alike (United States)

    Neff, John M.


    The arrival of summer signals a season of endless days of swimming, fishing, summer camps, and other outdoor activities. For children with chronic or terminal illnesses, it can be difficult to participate in many of these activities as well as challenging for parents to find summer camps that not only engage their children, but also offer the…

  18. Art Matters: The Creative Side of the Summer Camp Experience. (United States)

    Foster, Eden


    In western North Carolina, 24 summer camps, business leaders, and a local community arts council collaborated on a project celebrating the visual and performing arts created by campers. Campers' art in every media was displayed in Hendersonville to highlight the benefits of summer camps to the community, including their economic, educational,…

  19. Vision, Leadership, and Change: The Case of Ramah Summer Camps (United States)

    Reimer, Joseph


    In his retrospective essay, Seymour Fox (1997) identified "vision" as the essential element that shaped the Ramah camp system. I will take a critical look at Fox's main claims: (1) A particular model of vision was essential to the development of Camp Ramah; and (2) That model of vision should guide contemporary Jewish educators in creating Jewish…

  20. Residential Grief Camps: An Initial Phenomenological Study of Staff Perspectives (United States)

    Brown, Tiffany B.; Kimball, Thomas G.


    Research has focused primarily on the impact of death on family functioning and the stages and tasks of grief, though little attention has been given to grief camps or the experiences of those who work there. This study explored the experiences of staff at a four-day overnight children's grief camp. Eight participants reported their experience of…

  1. Novel cAMP targets in cell proliferation

    NARCIS (Netherlands)

    Kuiperij, Hinke Bertha


    cAMP is a second messenger that plays a role in a wide variety of biological processes, one of which is the regulation of cell proliferation. Adenylate cyclases generate cAMP in the cell upon activation, followed by binding to and activation of its direct targets, PKA and Epac. PKA is a protein kina

  2. Creating a Healthy Camp Community: A Nurse's Role. (United States)

    Lishner, Kris Miller; Bruya, Margaret Auld

    This book provides an organized, systematic overview of the basic aspects of health program management, nursing practice, and human relations issues in camp nursing. A foremost assumption is that health care in most camps needs improvement. Good health is dependent upon interventions involving social, environmental, and lifestyle factors that…

  3. Directed evolution of the Escherichia coli cAMP receptor protein at the cAMP pocket. (United States)

    Gunasekara, Sanjiva M; Hicks, Matt N; Park, Jin; Brooks, Cory L; Serate, Jose; Saunders, Cameron V; Grover, Simranjeet K; Goto, Joy J; Lee, Jin-Won; Youn, Hwan


    The Escherichia coli cAMP receptor protein (CRP) requires cAMP binding to undergo a conformational change for DNA binding and transcriptional regulation. Two CRP residues, Thr(127) and Ser(128), are known to play important roles in cAMP binding through hydrogen bonding and in the cAMP-induced conformational change, but the connection between the two is not completely clear. Here, we simultaneously randomized the codons for these two residues and selected CRP mutants displaying high CRP activity in a cAMP-producing E. coli. Many different CRP mutants satisfied the screening condition for high CRP activity, including those that cannot form any hydrogen bonds with the incoming cAMP at the two positions. In vitro DNA-binding analysis confirmed that these selected CRP mutants indeed display high CRP activity in response to cAMP. These results indicate that the hydrogen bonding ability of the Thr(127) and Ser(128) residues is not critical for the cAMP-induced CRP activation. However, the hydrogen bonding ability of Thr(127) and Ser(128) was found to be important in attaining high cAMP affinity. Computational analysis revealed that most natural cAMP-sensing CRP homologs have Thr/Ser, Thr/Thr, or Thr/Asn at positions 127 and 128. All of these pairs are excellent hydrogen bonding partners and they do not elevate CRP activity in the absence of cAMP. Taken together, our analyses suggest that CRP evolved to have hydrogen bonding residues at the cAMP pocket residues 127 and 128 for performing dual functions: preserving high cAMP affinity and keeping CRP inactive in the absence of cAMP.

  4. How to run a sports camp – legally speaking

    Directory of Open Access Journals (Sweden)

    Steven Monk


    Full Text Available Camps are a great way to keep kids active throughout the summer and to make money for the program sponsoring them. Planning is needed for a sports camp to be safe for both the camp personnel and the athletes, and to minimize legal negligence.  Having a risk management plan in place is important to insure that everyone is aware of the risks of participating. Background checks on camp personnel help ensure the camp leaders and coaches do not have a criminal background.  It is important to inspect the facilities/equipment to make sure they are safe to use.  Athletic trainers help with injuries that may occur with participation.  Supervisors make sure that everyone is accounted for and camp rules help the campers behave in an appropriate manner.  When planning activities, it is important to look at the skill level of the athletes. If all of these areas are covered, camps should be legally protected if an issue were to arise.

  5. Camp Sports Injuries: Analysis of Causes, Modes and Frequencies

    Directory of Open Access Journals (Sweden)

    Panagiota Papageorgiou


    Full Text Available The purpose of this study was the description of sports injuries sustained by campers at summer camps, aged 7-15 years. A sample of 8 camps from the Greek camp population participated in this sport injury surveillance study. Doctors and camp directors completed reports detailing the number of sports injuries events sustained and provided specific information about each event. During the period of the study, 337 sport injury reports were completed. A total of 237 (70.3% boys and 100 (29.7% girls reported having a sport injury. Age of campers sustaining a sport injury was 10-12 years old (60.8%. The frequency of sports injuries was highest during the first camp season. The leading causes of sports injuries in children’s were: falls, crushed by object, collision with other person and slips. Cut/scratch injuries were the most common diagnoses (38.9%. Football, basketball and volleyball were the most frequent sport activities for injuries. Reports based surveillance systems can be successfully used to conducts sport injury surveillance among children attending summer camps. Data collected via such systems can be used to calculate sports injury rates, to describe patterns of sport injury and to identify risk factors for camper – related sport injuries. The results provide necessary information to develop prevention interventions to decrease the number of youth whose camp experiences are negatively affected by sport injury.

  6. Imaging alterations of cardiomyocyte cAMP microdomains in disease

    Directory of Open Access Journals (Sweden)

    Alexander eFroese


    Full Text Available 3’,5’-cyclic adenosine monophosphate (cAMP is an important second messenger which regulates heart function by acting in distinct subcellular microdomains. Recent years have provided deeper mechanistic insights into compartmentalized cAMP signaling and its link to cardiac disease. In this mini review, we summarize newest developments in this field achieved by cutting-edge biochemical and biophysical techniques. We further compile the data from different studies into a bigger picture of so far uncovered alterations in cardiomyocyte cAMP microdomains which occur in compensated cardiac hypertrophy and chronic heart failure. Finally, future research directions and translational perspectives are briefly discussed.

  7. Understanding a Spiritual Youth Camp as a Consciousness Raising Group: The Effects of a Subculture's Communication. (United States)

    Schnell, Jim

    This paper defines a spiritual youth camp as a consciousness raising group. The camp, founded in 1956 as a community church camp, has been independent of any religious denomination since disassociating from the founding community church in 1986. Communication processes are described as they relate to primary aspects of the camp experience. Primary…

  8. Tying the Design of Your Camp Staff Training to the Delivery of Desired Youth Outcomes (United States)

    Galloway, Robin; Bourdeau, Virginia; Arnold, Mary; Nott, Brooke D.


    As experience camp directors, we've seen the challenges faced by young camp counselors and inexperienced staff. Evaluations from staff at many camps motivated us to help our people be more effective with their campers. In response we created a comprehensive camp staff training. Lessons showed staff what we wanted them to do and say as they…

  9. New York Operation: Military Kids Afterschool Universe Cosmic Camps (United States)

    Schaff, N.


    The Cornell Center for Radiophysics and Space Research education and public outreach program and the New York State Operation: Military Kids program partnered to plan four weekend "Cosmic Camps" for youth from military families using the NASA Afterschool Universe program.

  10. Nationalsozialistische Konzentrations- und Vernichtungslager National Socialist Concentration and Extermination Camps

    Directory of Open Access Journals (Sweden)

    Max Plassmann


    Full Text Available Das Buch stellt Organisationsformen, Zuständigkeiten und Politik hinter dem nationalsozialistischen Konzentrationslagersystem dar.This book presents organization, competences and policies underlying the system of National Socialist concentration camps.

  11. Building a Successful Middle School Outreach Effort: Microscopy Camp (United States)

    Penn, Lee R.; Flynn, Leslie; Johnson, Page


    Microscopy Camp program is designed to introduce acceptable representations of crystalline particles and their atomic structure to twelve-year-old middle school students at a developmental and educational stage.

  12. 14 CFR 91.1413 - CAMP: Responsibility for airworthiness. (United States)


    ..., including airframes, aircraft engines, propellers, rotors, appliances, and parts. (2) Maintaining its..., including airframes, aircraft engines, propellers, rotors, appliances, emergency equipment and parts, under... Ownership Operations Program Management § 91.1413 CAMP: Responsibility for airworthiness. (a) For...

  13. A summer day camp approach to adolescent weight loss. (United States)

    Southam, M A; Kirkley, B G; Murchison, A; Berkowitz, R I


    Twenty-five overweight adolescents completed a summer weight loss day camp program on the Stanford University campus. All participants attended camp four days per week for four hours to learn and practice eating and exercise skills conducive to weight loss. Parents met weekly to discuss the program content and to explore their role in their adolescent's weight management. At posttreatment, reductions were achieved in weight, percent overweight, and skinfold, with greater changes observed for the eight-week group than for the four-week group. Improvements were also evident in participants' self-reported habits and knowledge of weight management concepts. Parent and participant assessment of the camp experience was very positive. The results of the summer weight loss day camp suggest that an intensive program of eating and exercise habit instruction, practice, and monitoring, which allows the participants to remain in the home setting, may provide benefits not found in other more traditional approaches to adolescent weight loss.

  14. Cardiac cAMP: production, hydrolysis, modulation and detection

    Directory of Open Access Journals (Sweden)

    Cédric eBOULARAN


    Full Text Available Cyclic adenosine 3’,5’-monophosphate (cAMP modulates a broad range of biological processes including the regulation of cardiac myocyte contractile function where it constitutes the main second messenger for β-adrenergic receptors’ signaling to fulfill positive chronotropic, inotropic and lusitropic effects. A growing number of studies pinpoint the role of spatial organization of the cAMP signaling as an essential mechanism to regulate cAMP outcomes in cardiac physiology. Here, we will briefly discuss the complexity of cAMP synthesis and degradation in the cardiac context, describe the way to detect it and review the main pharmacological arsenal to modulate its availability.

  15. 14 CFR 91.1423 - CAMP: Maintenance organization. (United States)


    ... Operations Program Management § 91.1423 CAMP: Maintenance organization. (a) Each program manager who... maintenance, or alterations, must organize the performance of those functions so as to separate the...

  16. The Physics of Quidditch Summer Camp: An Interdisciplinary Approach (United States)

    Hammer, Donna; Uher, Tim

    The University of Maryland Physics Department has developed an innovative summer camp program that takes an interdisciplinary approach to engaging and teaching physics. The Physics of Quidditch Camp uniquely sits at the intersection of physics, sports, and literature, utilizing the real-life sport of quidditch adapted from the Harry Potter novels to stimulate critical thinking about real laws of physics and leaps of imagination, while actively engaging students in learning the sport and discussing the literature. Throughout the camp, middle school participants become immersed in fun physics experiments and exciting physical activities, which aim to build and enhance skills in problem-solving, analytical thinking, and teamwork. This camp has pioneered new ways of teaching physics to pre-college students, successfully engaged middle school students in learning physics, and grown a large demand for such activities.

  17. Science and technology camp for girls. Final report

    Energy Technology Data Exchange (ETDEWEB)


    This document reports on the success of Pacific University`s camp held during the summers of 1992 and 1993; ultimate goal of this summer day camp was to increase the number of women in technical and scientific fields. Some experimentation was done with the age groups (7th and 8th grade girls). The curriculum was biology, chemistry, physics, and mathematics/computer science. Laboratory work and field trips were emphasized, along with socialization.

  18. The Effect of a Nature Camp on Children’s Conceptions of Nature


    Esra Yardimci; Gulsen Leblebicioglu


    In this study, a nature camp which provides authentic learning opportunities for children was conducted. Twenty-four 4th and 5th graders (9 girls, 15 boys) participated to the camp. The camp program started with observations in the forest nearby. More focused observations were also made. Children discussed their observations with their friends and scientists. A questionnaire was applied at the beginning and end of the camp. The results showed that the nature camp program was effective in exte...

  19. Multiple facets of cAMP signalling and physiological impact : cAMP compartmentalization in the lung

    NARCIS (Netherlands)

    Oldenburger, Anouk; Maarsingh, Harm; Schmidt, Martina


    Therapies involving elevation of the endogenous suppressor cyclic AMP (cAMP) are currently used in the treatment of several chronic inflammatory disorders, including chronic obstructive pulmonary disease (COPD). Characteristics of COPD are airway obstruction, airway inflammation and airway remodelli

  20. Revisiting cAMP signaling in the carotid body

    Directory of Open Access Journals (Sweden)

    Ana Rita eNunes


    Full Text Available Chronic carotid body (CB activation is now recognized as being essential in the development of hypertension and promoting insulin resistance; thus, it is imperative to characterize the chemotransduction mechanisms of this organ in order to modulate its activity and improve patient outcomes. For several years, and although controversial, cyclic adenosine monophosphate (cAMP was considered an important player in initiating the activation of the CB. However, its relevance was partially displaced in the 90s by the emerging role of the mitochondria and molecules such as AMP-activated protein kinase (AMPK and O2-sensitive K+ channels. Neurotransmitters/neuromodulators binding to metabotropic receptors are essential to chemotransmission in the CB, and cAMP is central to this process. cAMP also contributes to raise intracellular Ca2+ levels, and is intimately related to the cellular energetic status (AMP/ATP ratio. Furthermore, cAMP signaling is a target of multiple current pharmacological agents used in clinical practice. This review provides an outline on 1 the classical view of the cAMP-signaling pathway in the CB that originally supported its role in the O2/CO2 sensing mechanism, 2 present recent evidence on CB cAMP neuromodulation and 3 discuss how CB activity is affected by current clinical therapies that modify cAMP-signaling, namely dopaminergic drugs, caffeine (modulation of A2A/A2B receptors and roflumilast (PDE4 inhibitors. cAMP is key to any process that involves metabotropic receptors and the intracellular pathways involved in CB disease states are likely to involve this classical second messenger. Research examining the potential modification of cAMP levels and/or interactions with molecules associated with CB hyperactivity is currently in its beginning and this review will open doors for future explorations.

  1. Emergency Medicine Residency Boot Camp Curriculum: A Pilot Study

    Directory of Open Access Journals (Sweden)

    Ataya, Ramsey


    Full Text Available Introduction: Establishing a boot camp curriculum is pertinent for emergency medicine (EM residents in order to develop proficiency in a large scope of procedures and leadership skills.  In this article, we describe our program’s EM boot camp curriculum as well as measure the confidence levels of resident physicians through a pre- and post-boot camp survey. Methods: We designed a one-month boot camp curriculum with the intention of improving the confidence, procedural performance, leadership, communication and resource management of EM interns. Our curriculum consisted of 12 hours of initial training and culminated in a two-day boot camp. The initial day consisted of clinical skill training and the second day included code drill scenarios followed by interprofessional debriefing.   Results: Twelve EM interns entered residency with an overall confidence score of 3.2 (1-5 scale across all surveyed skills. Interns reported the highest pre-survey confidence scores in suturing (4.3 and genitourinary exams (3.9. The lowest pre-survey confidence score was in thoracostomy (2.4. Following the capstone experience, overall confidence scores increased to 4.0. Confidence increased the most in defibrillation and thoracostomy. Additionally, all interns reported post-survey confidence scores of at least 3.0 in all skills, representing an internal anchor of “moderately confident/need guidance at times to perform procedure.” Conclusion: At the completion of the boot camp curriculum, EM interns had improvement in self-reported confidence across all surveyed skills and procedures. The described EM boot camp curriculum was effective, feasible and provided a foundation to our trainees during their first month of residency. [West J Emerg Med. 2015;16(2:356–361.

  2. Students' Perceptions of the Long-Term Impact of Attending a "CSI Science Camp" (United States)

    Yanowitz, Karen L.


    A science summer camp is a popular type of informal science experience for youth. While there is no one model of a science camp, these experiences typically allow for more focused and in-depth exploration of different science domains and are usually hands-on and participatory. The goal of this research was to examine the impact of a short science camp program approximately 1 year after students attended the camp. Overall, the results revealed that attending a 2-day forensic science camp had a positive and continuing influence on the participants. Students' science self-efficacy increased immediately after attending the camp and remained higher than pre-camp levels approximately 1 year later. Students were able to articulate why they believed the camp had a long-term impact on their lives. Furthermore, participants attributed a higher level of engaging in additional informal STEM-related activities during the academic year as a result of attending the camp.

  3. Students' Perceptions of the Long-Term Impact of Attending a "CSI Science Camp" (United States)

    Yanowitz, Karen L.


    A science summer camp is a popular type of informal science experience for youth. While there is no one model of a science camp, these experiences typically allow for more focused and in-depth exploration of different science domains and are usually hands-on and participatory. The goal of this research was to examine the impact of a short science camp program approximately 1 year after students attended the camp. Overall, the results revealed that attending a 2-day forensic science camp had a positive and continuing influence on the participants. Students' science self-efficacy increased immediately after attending the camp and remained higher than pre-camp levels approximately 1 year later. Students were able to articulate why they believed the camp had a long-term impact on their lives. Furthermore, participants attributed a higher level of engaging in additional informal STEM-related activities during the academic year as a result of attending the camp.

  4. Advances in Pediatric Cardiology Boot Camp: Boot Camp Training Promotes Fellowship Readiness and Enables Retention of Knowledge. (United States)

    Ceresnak, Scott R; Axelrod, David M; Sacks, Loren D; Motonaga, Kara S; Johnson, Emily R; Krawczeski, Catherine D


    We previously demonstrated that a pediatric cardiology boot camp can improve knowledge acquisition and decrease anxiety for trainees. We sought to determine if boot camp participants entered fellowship with a knowledge advantage over fellows who did not attend and if there was moderate-term retention of that knowledge. A 2-day training program was provided for incoming pediatric cardiology fellows from eight fellowship programs in April 2016. Hands-on, immersive experiences and simulations were provided in all major areas of pediatric cardiology. Knowledge-based examinations were completed by each participant prior to boot camp (PRE), immediately post-training (POST), and prior to the start of fellowship in June 2016 (F/U). A control group of fellows who did not attend boot camp also completed an examination prior to fellowship (CTRL). Comparisons of scores were made for individual participants and between participants and controls. A total of 16 participants and 16 control subjects were included. Baseline exam scores were similar between participants and controls (PRE 47 ± 11% vs. CTRL 52 ± 10%; p = 0.22). Participants' knowledge improved with boot camp training (PRE 47 ± 11% vs. POST 70 ± 8%; p < 0.001) and there was excellent moderate-term retention of the information taught at boot camp (PRE 47 ± 11% vs. F/U 71 ± 8%; p < 0.001). Testing done at the beginning of fellowship demonstrated significantly better scores in participants versus controls (F/U 71 ± 8% vs. CTRL 52 ± 10%; p < 0.001). Boot camp participants demonstrated a significant improvement in basic cardiology knowledge after the training program and had excellent moderate-term retention of that knowledge. Participants began fellowship with a larger fund of knowledge than those fellows who did not attend.

  5. The Popeye Domain Containing Genes and cAMP Signaling

    Directory of Open Access Journals (Sweden)

    Thomas Brand


    Full Text Available 3'-5'-cyclic adenosine monophosphate (cAMP is a second messenger, which plays an important role in the heart. It is generated in response to activation of G-protein-coupled receptors (GPCRs. Initially, it was thought that protein kinase A (PKA exclusively mediates cAMP-induced cellular responses such as an increase in cardiac contractility, relaxation, and heart rate. With the identification of the exchange factor directly activated by cAMP (EPAC and hyperpolarizing cyclic nucleotide-gated (HCN channels as cAMP effector proteins it became clear that a protein network is involved in cAMP signaling. The Popeye domain containing (Popdc genes encode yet another family of cAMP-binding proteins, which are prominently expressed in the heart. Loss-of-function mutations in mice are associated with cardiac arrhythmia and impaired skeletal muscle regeneration. Interestingly, the cardiac phenotype, which is present in both, Popdc1 and Popdc2 null mutants, is characterized by a stress-induced sinus bradycardia, suggesting that Popdc proteins participate in cAMP signaling in the sinuatrial node. The identification of the two-pore channel TREK-1 and Caveolin 3 as Popdc-interacting proteins represents a first step into understanding the mechanisms of heart rate modulation triggered by Popdc proteins.

  6. Evaluating the Effectiveness of the General Surgery Intern Boot Camp. (United States)

    Schoolfield, Clint S; Samra, Navdeep; Kim, Roger H; Shi, Runhua; Zhang, Wayne W; Tan, Tze-Woei


    The aim of our study is to evaluate the effectiveness of newly implemented general surgery intern boot camp. A 2-day didactic and skills-based intern boot camp was implemented before the start of clinical duties. Participants who did not attend all boot camp activities and had prior postgraduate training were excluded. A survey utilizing a 5-point Likert scale scoring system was used to assess the participants' confidence to perform intern-level tasks before and after the boot camp. Subgroup analyses were performed comparing changes in confidence among graduates from home institution versus others and general surgery versus other subspecialties. In the analysis, 21 participants over two years were included. Among them, 7 were graduates from home institution (4 general surgery, 3 subspecialty) and 14 were from other institutions (6 general surgery and 8 subspecialty). There were significant increases in overall confidence levels (pre = 2.79 vs post = 3.43, P surgery (2.78 vs 3.46, P = 0.001) and other specialties (2.74 vs 3.34, P surgery intern boot camp before the start of official rotation is effective in improving confidence level in performing level-appropriate tasks of the incoming new interns.

  7. Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions

    Directory of Open Access Journals (Sweden)

    Yan Xu


    Full Text Available 8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.

  8. Intracellular cAMP signaling by soluble adenylyl cyclase. (United States)

    Tresguerres, Martin; Levin, Lonny R; Buck, Jochen


    Soluble adenylyl cyclase (sAC) is a recently identified source of the ubiquitous second messenger cyclic adenosine 3',5' monophosphate (cAMP). sAC is distinct from the more widely studied source of cAMP, the transmembrane adenylyl cyclases (tmACs); its activity is uniquely regulated by bicarbonate anions, and it is distributed throughout the cytoplasm and in cellular organelles. Due to its unique localization and regulation, sAC has various functions in a variety of physiological systems that are distinct from tmACs. In this review, we detail the known functions of sAC, and we reassess commonly held views of cAMP signaling inside cells.

  9. Investigation of Sylvatic Typhus at a Wilderness Camp

    Centers for Disease Control (CDC) Podcasts


    In this podcast, Dr. Greg Dasch discusses an outbreak of four cases of sylvatic typhus that occurred at a wilderness camp in Pennsylvania. Sylvatic typhus is very rare in the United States, with only 41 cases since it was discovered in the United States in 1975. Lab work at CDC and the discovery that all four camp counselors who became ill had slept in the same bunk at the camp between 2004 and 2006 ultimately led to confirmation that flying squirrels living in the wall of the cabin were to blame for the illnesses.  Created: 6/30/2009 by Emerging Infectious Diseases.   Date Released: 6/30/2009.

  10. Rebuilding Jewish identities in Displaced Persons Camps in Germany

    Directory of Open Access Journals (Sweden)

    Françoise Ouzan


    Full Text Available In the summer 1945, Displaced Persons camps in Germany epitomized a place of contrasts and paradoxes. DPs still languished behind barbed wires after the Allied armies had liberated the concentration camps. The military had assumed that practically all of the Displaced Persons would be sent to their countries of origin. In the spring and summer 1945, 65 000 DPs were sent back home every day and almost six million were repatriated in September 1945. Accurate statistics are impossible, yet, acco...

  11. Science Camp - lystigt eller lærerigt

    DEFF Research Database (Denmark)

    Ahrenkiel, Linda; Albrechtsen, Thomas S. R.


    I oplægget vil vi undersøge fænomenet Science Camps nærmere ved at fortælle om dets historiske udvikling og ikke mindst lægge op til en diskussion af en definition. Derudover vil vi præsentere en case, hvor der med udgangspunkt i et aktuelt ph.d.-projekt er blevet undersøgt, hvad deltagerne får ud...... af at deltage i en science camp: Kan man både vække begejstring og medvirke til læring?...

  12. "Camping up" self-esteem in children with hemophilia. (United States)

    Thomas, D; Gaslin, T C


    Children with hemophilia have often been viewed at greater risk for altered self-esteem than their healthy counterparts. Our article shares the positive effects of the camp experience on children with hemophilia and subsequent enhancement of self-esteem. Interaction and support in the camp environment provide an opportunity for these children to gain independence and "prove" their self-worth and ability. Previous literature has provided a variety of findings on the relationship between chronic illness, such as hemophilia, and self-esteem alterations. We identify many opportunities for future education and research to provide quality nursing support to this unique population.

  13. EduCamp Colombia: Social Networked Learning for Teacher Training

    Directory of Open Access Journals (Sweden)

    Diego Ernesto Leal Fonseca


    Full Text Available This paper describes a learning experience called EduCamp, which was launched by the Ministry of Education of Colombia in 2007, based on emerging concepts such as e-Learning 2.0, connectivism, and personal learning environments. An EduCamp proposes an unstructured collective learning experience, which intends to make palpable the possibilities of social software tools in learning and interaction processes while demonstrating face-to-face organizational forms that reflect social networked learning ideas. The experience opens new perspectives for the design of technology training workshops and for the development of lifelong learning experiences.

  14. Science Skills Boot Camp Gets Interns Ready for Research | Poster (United States)

    By Ashley DeVine, Staff Writer Summer interns learned how to read a scientific paper, present a poster, maintain a laboratory notebook, and much more, at the Science Skills Boot Camp in June. “It was a great experience, and it was a great opportunity to meet some of the other interns also working on the campus,” said Alyssa Klein, a Werner H. Kirsten student intern in the Cellular Immunology Group, Laboratory of Molecular Immunoregulation. “The boot camp covered many topics essential to being a good scientist and science researcher.”

  15. Camp as a Teaching Method in Health Education

    DEFF Research Database (Denmark)

    Ringby, Betina

    methodology and theories/methods put INTO action. The camp method encourages a student-participatory and an inter-professionally approach required to think out-of-the-box. Teachers were offered the possibility to be mentor/coach for students. ‘Effectuation’, the ‘PUSH model’ and the ‘Entrepreneurial...... education to support students in gaining innovative and entrepreneurial skills. Participants A total of 33 physiotherapist students each participated in one of three CAMPS of 48, 24 or 12 hours in an elective module named “Sport, innovation and entrepreneurship”. Methods The project was based on case...

  16. Promoting independence in adolescent paraplegics: a 2-week "camping" experience. (United States)

    Bodzioch, J; Roach, J W; Schkade, J


    In the summer of 1982, Texas Scottish Rite Hospital (Dallas, TX, U.S.A.) sponsored a camp for paraplegic adolescents. Six patients, three boys and three girls 14-17 years of age, participated in a 2-week program that was designed to improve their self-esteem, independence, and eventual employability. In their pre- and postcamp psychological evaluations, the campers demonstrated improvement in social skills and self-concept testing as compared with the scores of a matched control group, although this improvement did not reach statistical significance. We believe the camp was immensely successful, an opinion that was shared by both the campers and their parents.

  17. Seafloor Science and Remotely Operated Vehicle (SSROV) Day Camp: A Week-Long, Hands-On STEM Summer Camp (United States)

    Wheat, C. G.; Fournier, T.; Monahan, K.; Paul, C.


    RETINA (Robotic Exploration Technologies IN Astrobiology) has developed a program geared towards stimulating our youth with innovative and relevant hands-on learning modules under a STEM umbrella. Given the breadth of potential science and engineering topics that excite children, the RETINA Program focuses on interactive participation in the design and development of simple robotic and sensor systems, providing a range of challenges to engage students through project-based learning (PBL). Thus, young students experience scientific discovery through the use and understanding of technology. This groundwork serves as the foundation for SSROV Camp, a week-long, summer day camp for 6th-8th grade students. The camp is centered on the sensors and platforms that guide seafloor exploration and discovery and builds upon the notion that transformative discoveries in the deep sea result from either sampling new environments or making new measurements with sensors adapted to this extreme environment. These technical and scientific needs are folded into the curriculum. Each of the first four days of the camp includes four team-based, hands-on technical challenges, communication among peer groups, and competition. The fifth day includes additional activities, culminating in camper-led presentations to describe a planned mission based on a given geologic setting. Presentations include hypotheses, operational requirements and expected data products. SSROV Camp was initiated last summer for three sessions, two in Monterey, CA and one in Oxford, MS. Campers from both regions grasped key elements of the program, based on written responses to questions before and after the camp. On average, 32% of the pre-test questions were answered correctly compared with 80% of the post-test questions. Additional confirmation of gains in campers' knowledge, skills, and critical thinking on environmental issues and engineering problems were apparent during the "jeopardy" competition, nightly homework

  18. The freshman camp report of Computer & Information Engineering(Educationai Development Symposiums)


    粂野, 文洋; Fumihiro, Kumeno


    In 2015 and 2016, the freshman camp of Computer & Information Engineering was conducted at Kinugawa Park Hotels, Nikko National Park. We successfully carried out the both camps in almost the same program and schedule. Based on this experience, the basic outline of our camp and the process for preparation and execution has been definite. In this report, we explain the basic outline and what we have done in each phase of the camp: plan, preparation, execution and finalizing. We also analyze the...

  19. Streptococcus pyogenes streptolysin O as a cause of false-positive CAMP reactions.


    Tapsall, J W; Phillips, E A


    The synergistic hemolysis of sheep erythrocytes in the CAMP reaction by the sequential action of staphylococcal beta-lysin and the CAMP factor of group B streptococci is the only known function of this extracellular product of group B streptococci. The reaction forms the basis of the CAMP test used to identify group B streptococci because the CAMP factor is believed to be restricted to this group of organisms. However, on occasion other streptococci, notably group A streptococci, may produce ...

  20. Hack City Summer: Computer Camps Can Bring a Vacation of Keyboard Delights. (United States)

    Shell, Ellen Ruppel


    Activities at a summer computer camp (Camp Atari held at East Stroudsburg State College PA) are described. The curriculum, using logic, systematic analysis, and other fundamental programing skills, teaches students to interact effectively and creatively with computers. Sources for finding a computer camp are included. (JN)

  1. Culture Camp, Ethnic Identity, and Adoption Socialization for Korean Adoptees: A Pretest and Posttest Study (United States)

    Baden, Amanda L.


    This study explores the impact of racial-ethnic socialization on adopted South Korean children and adolescents who attended a sleepaway Korean culture camp for one week. This camp provided racial-ethnic socialization experiences via exposure to camp counselors, staff, and teachers who were Korean Americans, Korean nationals, and Korean adult…

  2. The accidental city : violence, economy and humanitarianism in Kakuma refugee camp Kenya

    NARCIS (Netherlands)

    Jansen, B.J.


    In this research I examine social ordering processes in Kakuma refugee camp in Kenya. I view the camp as an accidental city, by which I challenge the image of the camp as a temporary and artificial waiting space or a protracted refugee crisis per se. The reference to the city is both metaphorical

  3. A Multidisciplinary Science Summer Camp for Students with Emphasis on Environmental and Analytical Chemistry (United States)

    Schwarz, Gunnar; Frenzel, Wolfgang; Richter, Wolfgang M.; Ta¨uscher, Lothar; Kubsch, Georg


    This paper presents the course of events of a five-day summer camp on environmental chemistry with high emphasis on chemical analysis. The annual camp was optional and open for students of all disciplines and levels. The duration of the summer camp was five and a half days in the Feldberg Lake District in northeast Germany (federal state of…

  4. 77 FR 56174 - Proposed Establishment of Class D and Class E Airspace; Camp Guernsey, WY (United States)


    ... 14 CFR Part 71 Proposed Establishment of Class D and Class E Airspace; Camp Guernsey, WY AGENCY... action proposes to establish Class D airspace and Class E airspace at Camp Guernsey Airport, Camp... holidays. An informal docket may also be examined during normal business hours at the Northwest...

  5. Reflections on Refugee Students' Major Perceptions of Education in Kakuma Refugee Camp, Kenya (United States)

    Mareng, Chuei D.


    This reflective study explores refugee students' perceptions of the educational approach used in Kakuma Refugee Camp in Kenya. The study focuses on my personal reflections as a teacher and a student in this camp, and as a refugee. My goal of writing this narrative is to reflect fully on the refugee students' life in a camp and then contribute to…

  6. History Matters: Children's Art Education inside the Japanese American Internment Camp (United States)

    Wenger, Gina Mumma


    What did art education look like within the confines of the Japanese American Internment Camp classrooms? Did the art education in the camps reflect the same curriculum that was being taught outside the camps and what other factors may have played a part in the students' experience? I propose that there were at least three significant…

  7. Antagonists of chemoattractants reveal separate receptors for cAMP, folic acid and pterin in Dictyostelium

    NARCIS (Netherlands)

    Haastert, Peter J.M. van; Wit, René J.W. de; Konijn, Theo M.


    Adenosine 3’,5’-monophosphate (cAMP), folic acid and pterin are chemoattractants in the cellular slime molds. The cAMP analog, 3’-amino-cAMP, inhibits a chemotactic reaction to cAMP at a concentration at which the analog is chemotactically inactive. The antagonistic effect of 3’-amino-cAMP on the ch

  8. Activation of FoxO transcription factors contributes to the antiproliferative effect of cAMP

    NARCIS (Netherlands)

    Kuiperij, H.B.; Horst, Armando van der; Raaijmakers, Judith; Weijzen, S.; Medema, R.H.; Bos, J.L.; Burgering, B.M.T.; Zwartkruis, G.J.T.


    cAMP is a potent inhibitor of cell proliferation in a variety of cell lines. Downregulation of cyclin D1 and upregulation of the cell cycle inhibitor p27Kip1 are two mechanisms by which cAMP may induce a G1-arrest. Here we show that cAMP inhibits proliferation of cells that constitutively express cy

  9. What Do Children Most Enjoy about Summer Soccer Camp? Gender and Group Perceptions (United States)

    Jones, Rhys


    One hundred children attending a summer soccer camp in NE Ohio provided written data on what they most enjoyed about the camp. Findings indicated that, overall, they ranked "soccer games and skills" and "camp related activities" as the two leading major categories. In terms of gender group analysis (females = 49; males = 51)…

  10. 78 FR 55671 - Hospital Care and Medical Services for Camp Lejeune Veterans (United States)


    ... the residences and workplaces, in addition to other water systems on Camp Lejeune, have tested... substances while at Camp Lejeune. U.S. Marine Corps Base Camp Lejeune includes base housing, training sites..., 2015. We believe that 2 years would provide veterans sufficient time to learn about the new status...

  11. Elevated cAMP increases aquaporin-3 plasma membrane diffusion

    DEFF Research Database (Denmark)

    Marlar, Saw; Christensen, Eva Arnspang; Koffman, Jennifer Skaarup


    .05)]. Immunoelectron microscopy showed no obvious difference in AQP3-EGFP expression levels or localization in the plasma membrane upon forskolin stimulation. Thus AQP3-EGFP diffusion is altered upon increased cAMP, which may correspond to basolateral adaptations in response to the increased apical water readsorption...

  12. Phun Physics 4 Phemales: Physics Camp for High School Girls (United States)

    Kwon, Chuhee; Gu, Jiyeong; Henriquez, Laura


    The department of Physics and Astronomy with the department of Science Education at California State University, Long Beach hosted summer program of ``Phun Physics 4 Phemales (PP4P)'' during summer 2012 and summer 2013 with the support from APS public outreach program. PP4P summer camp was hosted along with a two-week summer science camp, Young Scientists Camp, which has been institutionalized for the last 14 years since 1999. More than 2,500 3rd -8th grade students and 250 teachers have participated in the program. PP4P program provided the tools and support that female high school students need to pursue careers in physics and/or science, technology, engineering and math (STEM) field. This girls-only camp created connections among the girls and built confidence. In addition PP4P program introduced students to key principles in physics by a hands-on lab environment and demonstrated the real-world social impact of physics. In summer 2012, high school girls worked on physics experimental project on electronics and in summer 2013 they worked on the mechanics. I would share our experience in this program and the impact on the female high school students. This work was supported by 2012 Public Outreach and Informing the Public Grants from American Physical Society.

  13. Addressing Nature Deficit Disorder through Primitive Camping Experiences (United States)

    Allen, Kevin; Varner, Keegan; Sallee, Jeff


    Today's youth suffer from Nature Deficit Disorder, a condition that has been connected to ADHD, shortage of creativity, and general lack of knowledge about the outdoors. A team of educators and specialists are addressing this issue with primitive camping. County educators were trained using experiential learning and train-the-trainer techniques.…

  14. Credit-based livelihood interventions in a Zambian refugee camp

    Directory of Open Access Journals (Sweden)

    Jane Travis


    Full Text Available Establishing community credit facilities has become an important developmental tool for building livelihood strategies. In the refugee camps where the British NGO Christian Outreach Relief andDevelopment (CORD has worked, programmes have provided credit in the form of cash, agricultural inputs or livestock.

  15. Snakes Have Feelings, Too: Elements of a Camp Snake Program. (United States)

    Allen, Robert Ross


    A camp snake program can help campers overcome their fear of snakes, and people cannot truly enjoy nature when they carry a phobia about any one part of it. It can also help overcome prejudice by teaching truth and respect, instilling compassion, and helping campers develop empathy. Advice on catching, handling, identifying, keeping, and feeding…

  16. A Pediatric Cardiology Fellowship Boot Camp improves trainee confidence. (United States)

    Allan, Catherine K; Tannous, Paul; DeWitt, Elizabeth; Farias, Michael; Mansfield, Laura; Ronai, Christina; Schidlow, David; Sanders, Stephen P; Lock, James E; Newburger, Jane W; Brown, David W


    Introduction New paediatric cardiology trainees are required to rapidly assimilate knowledge and gain clinical skills to which they have limited or no exposure during residency. The Pediatric Cardiology Fellowship Boot Camp (PCBC) at Boston Children's Hospital was designed to provide incoming fellows with an intensive exposure to congenital cardiac pathology and a broad overview of major areas of paediatric cardiology practice.

  17. The Career Camp: An Early, Early Career Guidance Program. (United States)

    Wright, Sue Edmonds


    Describes Marshall University's summer camp where young people participate in activities that help prepare them for important career choices they will need to make when entering college. Benefits for the university include: recruitment, enhanced community partnerships, better informed incoming freshman, and publicity for the career center. (JBJ)

  18. Camping Burner-Based Flame Emission Spectrometer for Classroom Demonstrations (United States)

    Ne´el, Bastien; Crespo, Gasto´n A.; Perret, Didier; Cherubini, Thomas; Bakker, Eric


    A flame emission spectrometer was built in-house for the purpose of introducing this analytical technique to students at the high school level. The aqueous sample is sprayed through a homemade nebulizer into the air inlet of a consumer-grade propane camping burner. The resulting flame is analyzed by a commercial array spectrometer for the visible…

  19. Novel H1N1 Flu and Camp

    Centers for Disease Control (CDC) Podcasts


    This podcast gives tips to stay healthy and help prevent infection with novel H1N1 flu if your child or someone you know is going to camp.  Created: 6/30/2009 by Centers for Disease Control and Prevention (CDC).   Date Released: 6/30/2009.

  20. Archaeology and Memory. Former WWII Camps in Europe

    NARCIS (Netherlands)

    Laarse, van der R.; Ooijen, van I.M.A.

    The archaeology of 20th-century war, terror and conflict is a growing field of research. The archaeological research of ‘terrorscapes’ often overlaps with personal and collective memories. Besides memory, the heritage of the camps has been dominated in the last decades by historical research. What t

  1. Menselijk gedrag bij de evacuatie van een camping

    NARCIS (Netherlands)

    Kerstholt, J.H.; Groenewegen-Ter Morsche, K.; Johannink, R.; Getz-Smeenk, A.F.


    In geval van onbeheersbare natuurbranden zal er een beroep moeten worden gedaan op de zelfredzaamheid van burgers. De resultaten van een ontruiming van een camping laten zien dat vele deelnemers niet reageerden op rook en brandgeur en dat ze slechts in actie kwamen nadat z|j door een personeelslid v

  2. Academic Boot Camp for the Writing of Psychology Research Reports (United States)

    Skues, Jason L.; Wise, Lisa


    Herein, we describe the implementation of, and responses to, a structured writing workshop in the form of an academic boot camp. Participants were 42 undergraduate psychology students from a medium-sized Australian university who were completing their major assignment for the semester. A majority of the students expressed satisfaction with the…

  3. Chinese Students’ Winter Camp Tour of the U. S.

    Institute of Scientific and Technical Information of China (English)


    <正>At the invitation of Brigham Young University (BYU)-Hawaii of the U. S., a 26-member middle school student winter camp delegation, organized and sent by the CPAFFC, made a study tour of the U. S. with the theme of enhancing friendship between the

  4. Graying America Presents Golden Opportunities for Camp Directors. (United States)

    Cosky, Alicia C.


    Discusses quality-of-life issues for ever-increasing population of American elderly, emphasizing value of recreation. Offers organized camping as way of exposing older adults to enjoyable physical activities. Cites evidence supporting beneficial effects of regular exercise for elderly, beginning at any age. (TES)

  5. Camp stability predicts patterns of hunter-gatherer cooperation. (United States)

    Smith, Daniel; Dyble, Mark; Thompson, James; Major, Katie; Page, Abigail E; Chaudhary, Nikhil; Salali, Gul Deniz; Vinicius, Lucio; Migliano, Andrea Bamberg; Mace, Ruth


    Humans regularly cooperate with non-kin, which has been theorized to require reciprocity between repeatedly interacting and trusting individuals. However, the role of repeated interactions has not previously been demonstrated in explaining real-world patterns of hunter-gatherer cooperation. Here we explore cooperation among the Agta, a population of Filipino hunter-gatherers, using data from both actual resource transfers and two experimental games across multiple camps. Patterns of cooperation vary greatly between camps and depend on socio-ecological context. Stable camps (with fewer changes in membership over time) were associated with greater reciprocal sharing, indicating that an increased likelihood of future interactions facilitates reciprocity. This is the first study reporting an association between reciprocal cooperation and hunter-gatherer band stability. Under conditions of low camp stability individuals still acquire resources from others, but do so via demand sharing (taking from others), rather than based on reciprocal considerations. Hunter-gatherer cooperation may either be characterized as reciprocity or demand sharing depending on socio-ecological conditions.

  6. Using Science Camps to Develop Understandings about Scientific Inquiry--Taiwanese Students in a U.S. Summer Science Camp (United States)

    Antink-Meyer, Allison; Bartos, Stephen; Lederman, Judith S.; Lederman, Norman G.


    Recent years have witnessed a dramatic rise in the number of middle and high school students from Asian countries participating in U.S.-based summer experiences (Perlez & Gao, 2013). Although summer science camps have been shown to improve students' attitudes and interests related to science and science learning (Bhattacharyya, Mead &…

  7. Novel cAMP signalling paradigms: therapeutic implications for airway disease


    Billington, Charlotte K; Hall, Ian P


    Since its discovery over 50 years ago, cAMP has been the archetypal second messenger introducing students to the concept of cell signalling at the simplest level. As explored in this review, however, there are many more facets to cAMP signalling than the path from Gs-coupled receptor to adenylyl cyclase (AC) to cAMP to PKA to biological effect. After a brief description of this canonical cAMP signalling pathway, a snapshot is provided of the novel paradigms of cAMP signalling. As in the airwa...

  8. Inactivation of Multidrug Resistance Proteins Disrupts Both Cellular Extrusion and Intracellular Degradation of cAMP


    Xie, Moses; Rich, Thomas C.; Scheitrum, Colleen; Conti, Marco; Richter, Wito


    In addition to xenobiotics and several other endogenous metabolites, multidrug-resistance proteins (MRPs) extrude the second-messenger cAMP from various cells. Pharmacological and/or genetic inactivation of MRPs has been shown to augment intracellular cAMP signaling, an effect assumed to be a direct consequence of the blockade of cAMP extrusion. Here we provide evidence that the augmented intracellular cAMP levels are not due exclusively to the prevention of cAMP efflux because MRP inactivati...

  9. Multiple Facets of cAMP Signalling and Physiological Impact: cAMP Compartmentalization in the Lung

    Directory of Open Access Journals (Sweden)

    Martina Schmidt


    Full Text Available Therapies involving elevation of the endogenous suppressor cyclic AMP (cAMP are currently used in the treatment of several chronic inflammatory disorders, including chronic obstructive pulmonary disease (COPD. Characteristics of COPD are airway obstruction, airway inflammation and airway remodelling, processes encompassed by increased airway smooth muscle mass, epithelial changes, goblet cell and submucosal gland hyperplasia. In addition to inflammatory cells, airway smooth muscle cells and (myofibroblasts, epithelial cells underpin a variety of key responses in the airways such as inflammatory cytokine release, airway remodelling, mucus hypersecretion and airway barrier function. Cigarette smoke, being next to environmental pollution the main cause of COPD, is believed to cause epithelial hyperpermeability by disrupting the barrier function. Here we will focus on the most recent progress on compartmentalized signalling by cAMP. In addition to G protein-coupled receptors, adenylyl cyclases, cAMP-specific phospho-diesterases (PDEs maintain compartmentalized cAMP signalling. Intriguingly, spatially discrete cAMP-sensing signalling complexes seem also to involve distinct members of the A-kinase anchoring (AKAP superfamily and IQ motif containing GTPase activating protein (IQGAPs. In this review, we will highlight the interaction between cAMP and the epithelial barrier to retain proper lung function and to alleviate COPD symptoms and focus on the possible molecular mechanisms involved in this process. Future studies should include the development of cAMP-sensing multiprotein complex specific disruptors and/or stabilizers to orchestrate cellular functions. Compartmentalized cAMP signalling regulates important cellular processes in the lung and may serve as a therapeutic target.

  10. [Medicine in the concentration camps of the Third Reich]. (United States)

    Shasha, Shaul M


    Between 1942 and 1944 millions of prisoners were subjected to forced labor in concentration camps throughout the Third Reich, all the while being the victims of a systematic and "scientific" extermination policy. Though the policy was directed mainly against Jews, it was implemented against other "inferior races" as well. The prisoners, stripped of all rights, experienced constant humiliation, uncertain survival and terror. The harsh living condition, characterized by crowding, absent sanitation and poor personal hygiene led to considerable morbidity, mainly due to injuries, infectious diseases and famine, and to high mortality rates. Medical care in the camps was the responsibility of the S.S. Each camp had a chief S.S. physician accompanied by a number of assistants and orderlies. There was also a parallel system of "prisoner-physicians." There was a chief prisoner-physician in every camp, and each block was assigned a "block doctor" who was responsible for sanitation, the removal of corpses, setting up the sick- call and authorizing sick leave. Work teams were accompanied by "mobile doctors" (Streckenpfleger), who dispensed first aid for work injuries. Prisoner-physicians were also charged with disinfecting the blocks and maintaining hygienic conditions in the camp. Every camp had one or more blocks, called "Reviers", that were used for treatment and hospitalization. In the larger camps a number of blocks were designated to function as a sort of hospital (Krankenbau). At times one camp out of a group of camps would be set aside as quarantine, primarily for patients with infectious diseases. Officially, the "Revier" was the responsibility of an S.S. physicians', assisted by a chief prisoner-physician, his assistants and, at times, nurses. But in actuality the Reviers were managed by prisoners (Capos) who did not have medical training but were authorized to make decisions in medical matters such as operations and, on occasion, even performed them. The Reviers

  11. Streptococcus pyogenes CAMP factor attenuates phagocytic activity of RAW 264.7 cells. (United States)

    Kurosawa, Mie; Oda, Masataka; Domon, Hisanori; Saitoh, Issei; Hayasaki, Haruaki; Terao, Yutaka


    Streptococcus pyogenes produces molecules that inhibit the function of human immune system, thus allowing the pathogen to grow and spread in tissues. It is known that S. pyogenes CAMP factor increases erythrocytosis induced by Staphylococcus aureus β-hemolysin. However, the effects of CAMP factor for immune cells are unclear. In this study, we investigated the effects of CAMP factor to macrophages. Western blotting analysis demonstrated that all examined strains expressed CAMP factor protein. In the presence of calcium or magnesium ion, CAMP factor was significantly released in the supernatant. In addition, both culture supernatant from S. pyogenes strain SSI-9 and recombinant CAMP factor dose-dependently induced vacuolation in RAW 264.7 cells, but the culture supernatant from Δcfa isogenic mutant strain did not. CAMP factor formed oligomers in RAW 264.7 cells in a time-dependent manner. CAMP factor suppressed cell proliferation via G2 phase cell cycle arrest without inducing cell death. Furthermore, CAMP factor reduced the uptake of S. pyogenes and phagocytic activity indicator by RAW 264.7 cells. These results suggest that CAMP factor works as a macrophage dysfunction factor. Therefore, we conclude that CAMP factor allows S. pyogenes to escape the host immune system, and contribute to the spread of streptococcal infection.

  12. From camp to kitsch: A queer eye on console fandom

    Directory of Open Access Journals (Sweden)

    Rob Gallagher


    Full Text Available Offering a queer perspective on video game fandom, this article considers the factors that fostered a subculture of Western devotees of Japanese video games in the 1990s. Focused on readers of the English publication Sega Saturn Magazine, it shows how, for these players, Japanese games became the basis of a collective identity founded on precisely the kinds of perverse over-attachment, projective identification and hermeneutic ingenuity that Eve Kosofsky Sedgwick identifies with camp. Citing this subculture as an example of how fans transform the texts they put to use, the article also addresses its implications for our understanding of fandom today, at a time when the proliferation of quantitative analysis techniques is transforming the production and consumption of games. Such techniques, I argue, threaten to compromise the contingency and ambiguity on which camp thrives, instead fostering the kinds of cynical calculation Sedgwick associates with kitsch.

  13. Collaborative Tools Used to Organize a Library Camp Unconference


    Crossett, Laura; Kraus, Joseph; Lawson, Steve


    From July to October, 2008, Laura Crossett, Joseph Kraus and Steve Lawson organized the Library Camp of the West ( This was an unconference that took place on October 10, 2008 at the University of Denver. The authors used many technology tools to organize the event, such as email, wikis, blogs, two tools from Google, the Doodle scheduling Website, Flickr and more. This article will explain how they used those tools to prepare for the unconference.


    Directory of Open Access Journals (Sweden)

    Antony R.


    Full Text Available The study was intended to assess the results of soft tissue release and bony corrective surgery in patients of moderate to severe deformed rigid club foot (CTEV and neglected clubfoot (CTEV at free disabled surgical camps at Chhattisgarh state . MATERIAL AND METHODS : In our study 50 patients were included with 70% male and 30% female with 4 - 16 years of age grou p and 70% unilateral and 30% bilateral foot involvement. Patients were admitted and operated in different free disabled surgical camps at Chhattisgarh state over the period of 36 months (1 may 2004 to 30 th April 2007. Improvement in functional ability and locomotion of all operated patients were assessed by physical and clinical examination. RESULTS : All patients who were operated in our study showed significant improvement in functional ability and locomotion after surgery. All patients were maintaining f unctional ability at follow up duration of 12 months (1 year. 75% patients were walking normally, 10% cases were walking with internal rotation of leg and 5% cases were walking with midtarsal varus foot with AFO with medial bar support. CONCLUSION : Our st udy showed and established that excellent results can be obtained in congenital talipes equinovarus (CTEV patients by soft tissue release with bony corrective surgery. The team work of devoted surgeons, paramedical and rehabilitation staff in whole durati on of camps to achieve the goal. With an aim to help more number of CTEV cases by surgery, our team has started doing surgeries in small institutions, and organize charity camps to help poor patients and mankind even in small clinics

  15. Camp Pendleton Saves 91% in Parking Lot Lighting

    Energy Technology Data Exchange (ETDEWEB)



    Case study describes how Camp Pendleton Marine Corps Base replaced high-pressure sodium (HPS) fixtures in one parking lot with high-efficiency induction fixtures for 91% savings in energy use and $5,700 in cost savings annually. This parking lot is estimated to have a simple payback of 2.9 years. Sitewide up-grades yielded annual savings of 1 million kWh.

  16. ESTCP Live Site Demonstrations Former Camp Beale, Marysville, CA (United States)


    31  Figure 10-1 – Management and Staffing Wiring Diagram . ...........................................................32  Tables Table...the completion of the demonstration. The schedule of field testing activities is provided in Figure 5-1 as a Gantt chart. Activity Name 5 12 19...Management and Staffing Wiring Diagram . 32 33 11.0 REFERENCES 1. “ESTCP Munitions Response, Live Site Demonstrations, former Camp Beale, CA

  17. Building Energy Audit Report for Camp Smith, HI

    Energy Technology Data Exchange (ETDEWEB)

    Chvala, William D.; De La Rosa, Marcus I.; Brown, Daryl R.; Dixon, Douglas R.


    A detailed energy assessment was performed by a team of engineers from Pacific Northwest National Laboratory (PNNL) under contract to the Department of Energy/Federal Energy Management program (FEMP). The effort used the Facility Energy Decision System (FEDS) model to determine how energy is consumed at Camp Smith, identify the most cost-effective energy retrofit measures, and calculate the potential energy and cost savings. This report documents the results of that assessment.

  18. Tidal Marsh Vegetation of China Camp, San Pablo Bay, California


    Baye, Peter R.


    China Camp (Marin County, California) preserves extensive relict stands of salt marsh vegetation developed on a prehistoric salt marsh platform with a complex sinuous tidal creek network. The low salt marsh along tidal creeks supports extensive native stands of Pacific cordgrass (Spartina foliosa). The outer salt marsh accreted following hydraulic gold mining sedimentation. It consists of a wave-scarped pickleweed-dominated (Sarcocornia pacifica) high salt marsh terrace with a broad fringing ...

  19. Smart and Green Energy (SAGE) for Base Camps Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Engels, Matthias; Boyd, Paul A.; Koehler, Theresa M.; Goel, Supriya; Sisk, Daniel R.; Hatley, Darrel D.; Mendon, Vrushali V.; Hail, John C.


    The U.S. Army Logistics Innovation Agency’s (LIA’s) Smart and Green Energy (SAGE) for Base Camps project was to investigate how base camps’ fuel consumption can be reduced by 30% to 60% using commercial off-the-shelf (COTS) technologies for power generation, renewables, and energy efficient building systems. Field tests and calibrated energy models successfully demonstrated that the fuel reductions are achievable.

  20. Flame Retardant Applications in Camping Tents and Potential Exposure


    Keller, Alexander S.; Raju, Nikhilesh P.; Webster, Thomas F.; Stapleton, Heather M.


    Concern has mounted over health effects caused by exposure to flame retardant additives used in consumer products. Significant research efforts have focused particularly on exposure to polybrominated diphenyl ethers (PBDEs) used in furniture and electronic applications. However, little attention has focused on applications in textiles, particularly textiles meeting a flammability standard known as CPAI-84. In this study, we investigated flame retardant applications in camping tents that met C...

  1. Exchange Protein Directly Activated by cAMP (epac) : A Multidomain cAMP Mediator in the Regulation of Diverse Biological Functions

    NARCIS (Netherlands)

    Schmidt, Martina; Dekker, Frank J.; Maarsingh, Harm


    Since the discovery nearly 60 years ago, cAMP is envisioned as one of the most universal and versatile second messengers. The tremendous feature of cAMP to tightly control highly diverse physiologic processes, including calcium homeostasis, metabolism, secretion, muscle contraction, cell fate, and g

  2. What Do Students Gain from a Week at Science Camp? Youth Perceptions and the Design of an Immersive, Research-Oriented Astronomy Camp (United States)

    Fields, Deborah Anne


    This study explored American high school students' perceptions of the benefits of a summer astronomy camp, emphasizing a full cycle of the research process and how the organization of the camp contributed to those perceptions. Semi-structured interviews with students and staff were used to elicit the specific benefits that campers perceived from…

  3. The Effect of a Disability Camp Program on Attitudes towards the Inclusion of Children with Disabilities in a Summer Sport and Leisure Activity Camp (United States)

    Papaioannou, Christina; Evaggelinou, Christina


    The aim of the present study was to examine the impact of a specific Disability Camp Program (DCP) in the attitudes of children without disabilities toward the inclusion of children with disabilities in a summer sport and leisure activity camp. Three hundred eighty-seven campers without disabilities participated in the study and were divided into…

  4. Developing Social Skills of Summer Campers with Autism Spectrum Disorder: A Case Study of Camps on TRACKS Implementation in an Inclusive Day-Camp Setting (United States)

    Maich, Kimberly; Hall, Carmen L.; van Rhijn, Tricia Marie; Quinlan, Laurie


    This research provides preliminary results of an exploratory case study conducted of the Camps on TRACKS program in an inclusive, municipal day-camp program in southwestern Ontario, Canada. Positive changes are demonstrated in the social skills of nine day campers with an autism spectrum disorder (ASD) who participated in the program. In this…

  5. Outbreak of chickenpox in a refugee camp of northern Thailand

    Directory of Open Access Journals (Sweden)

    Camélique Olivier


    Full Text Available Abstract Although chickenpox is a generally mild, self-limited illness of children, it can cause fatal disease in adults. Accumulating reports from tropical countries showed a high prevalence of seronegativity among the adults, implying that varicella diseases could become a heavy burden in tropical countries. However, in the situation of humanitarian emergencies in tropical areas, chickenpox has largely been ignored as a serious communicable disease, due to lack of data regarding varicella mortality and hospital admissions in such a context. This is the first report describing an outbreak of chickenpox in a refugee camp of tropical region. In 2008, we experienced a varicella outbreak in ethnic Lao Hmong refugee camp in Phetchabun Province, northern Thailand. The attack rate was 4.0% (309/7,815 and this caused 3 hospitalizations including one who developed severe varicella pneumonia with respiratory failure. All hospitalizations were exclusively seen in adults, and the proportion of patients ≥15 years old was 13.6% (42/309. Because less exposure to varicella-zoster virus due to low population density has previously been suggested to be one of the reasons behind higher prevalence of susceptible adults in tropics, the influx of displaced people from rural areas to a densely populated asylum might result in many severe adult cases once a varicella outbreak occurs. Control interventions such as vaccination should be considered even in refugee camp, if the confluence of the risk factors present in this situation.

  6. Biological roles of cAMP: variations on a theme in the different kingdoms of life. (United States)

    Gancedo, Juana M


    Cyclic AMP (cAMP) plays a key regulatory role in most types of cells; however, the pathways controlled by cAMP may present important differences between organisms and between tissues within a specific organism. Changes in cAMP levels are caused by multiple triggers, most affecting adenylyl cyclases, the enzymes that synthesize cAMP. Adenylyl cyclases form a large and diverse family including soluble forms and others with one or more transmembrane domains. Regulatory mechanisms for the soluble adenylyl cyclases involve either interaction with diverse proteins, as happens in Escherichia coli or yeasts, or with calcium or bicarbonate ions, as occurs in mammalian cells. The transmembrane cyclases can be regulated by a variety of proteins, among which the α subunit and the βγ complex from G proteins coupled to membrane receptors are prominent. cAMP levels also are controlled by the activity of phosphodiesterases, enzymes that hydrolyze cAMP. Phosphodiesterases can be regulated by cAMP, cGMP or calcium-calmodulin or by phosphorylation by different protein kinases. Regulation through cAMP depends on its binding to diverse proteins, its proximal targets, this in turn causing changes in a variety of distal targets. Specifically, binding of cAMP to regulatory subunits of cAMP-dependent protein kinases (PKAs) affects the activity of substrates of PKA, binding to exchange proteins directly activated by cAMP (Epac) regulates small GTPases, binding to transcription factors such as the cAMP receptor protein (CRP) or the virulence factor regulator (Vfr) modifies the rate of transcription of certain genes, while cAMP binding to ion channels modulates their activity directly. Further studies on cAMP signalling will have important implications, not only for advancing fundamental knowledge but also for identifying targets for the development of new therapeutic agents.

  7. Three-dimensional measurement of cAMP gradients using hyperspectral confocal microscopy (United States)

    Rich, Thomas C.; Annamdevula, Naga; Britain, Andrea L.; Mayes, Samuel; Favreau, Peter F.; Leavesley, Silas J.


    Cyclic AMP (cAMP) is a ubiquitous second messenger known to differentially regulate many cellular functions over a wide range of timescales. Several lines of evidence have suggested that the distribution of cAMP within cells is not uniform, and that cAMP compartmentalization is largely responsible for signaling specificity within the cAMP signaling pathway. However, to date, no studies have experimentally measured three dimensional (3D) cAMP distributions within cells. Here we use both 2D and 3D hyperspectral microscopy to visualize cAMP gradients in endothelial cells from the pulmonary microvasculature (PMVECs). cAMP levels were measured using a FRETbased cAMP sensor comprised of a cAMP binding domain from EPAC sandwiched between FRET donors and acceptors -- Turquoise and Venus fluorescent proteins. Data were acquired using either a Nikon A1R spectral confocal microscope or custom spectral microscopy system. Analysis of hyperspectral image stacks from a single confocal slice or from summed images of all slices (2D analysis) indicated little or no cAMP gradients were formed within PMVECs under basal conditions or following agonist treatment. However, analysis of hyperspectral image stacks from 3D cellular geometries (z stacks) demonstrate marked cAMP gradients from the apical to basolateral membrane of PMVECs. These results strongly suggest that 2D imaging studies of cAMP compartmentalization -- whether epifluorescence or confocal microscopy -- may lead to erroneous conclusions about the existence of cAMP gradients, and that 3D studies are required to assess mechanisms of signaling specificity.

  8. A Computational Modeling and Simulation Approach to Investigate Mechanisms of Subcellular cAMP Compartmentation. (United States)

    Yang, Pei-Chi; Boras, Britton W; Jeng, Mao-Tsuen; Docken, Steffen S; Lewis, Timothy J; McCulloch, Andrew D; Harvey, Robert D; Clancy, Colleen E


    Subcellular compartmentation of the ubiquitous second messenger cAMP has been widely proposed as a mechanism to explain unique receptor-dependent functional responses. How exactly compartmentation is achieved, however, has remained a mystery for more than 40 years. In this study, we developed computational and mathematical models to represent a subcellular sarcomeric space in a cardiac myocyte with varying detail. We then used these models to predict the contributions of various mechanisms that establish subcellular cAMP microdomains. We used the models to test the hypothesis that phosphodiesterases act as functional barriers to diffusion, creating discrete cAMP signaling domains. We also used the models to predict the effect of a range of experimentally measured diffusion rates on cAMP compartmentation. Finally, we modeled the anatomical structures in a cardiac myocyte diad, to predict the effects of anatomical diffusion barriers on cAMP compartmentation. When we incorporated experimentally informed model parameters to reconstruct an in silico subcellular sarcomeric space with spatially distinct cAMP production sites linked to caveloar domains, the models predict that under realistic conditions phosphodiesterases alone were insufficient to generate significant cAMP gradients. This prediction persisted even when combined with slow cAMP diffusion. When we additionally considered the effects of anatomic barriers to diffusion that are expected in the cardiac myocyte dyadic space, cAMP compartmentation did occur, but only when diffusion was slow. Our model simulations suggest that additional mechanisms likely contribute to cAMP gradients occurring in submicroscopic domains. The difference between the physiological and pathological effects resulting from the production of cAMP may be a function of appropriate compartmentation of cAMP signaling. Therefore, understanding the contribution of factors that are responsible for coordinating the spatial and temporal

  9. Chlorella intake attenuates reduced salivary SIgA secretion in kendo training camp participants

    Directory of Open Access Journals (Sweden)

    Otsuki Takeshi


    Full Text Available Abstract Background The green alga Chlorella contains high levels of proteins, vitamins, and minerals. We previously reported that a chlorella-derived multicomponent supplement increased the secretion rate of salivary secretory immunoglobulin A (SIgA in humans. Here, we investigated whether intake of this chlorella-derived supplement attenuated the reduced salivary SIgA secretion rate during a kendo training camp. Methods Ten female kendo athletes participated in inter-university 6-day spring and 4-day summer camps. They were randomized into two groups; one took placebo tablets during the spring camp and chlorella tablets during the summer camp, while the other took chlorella tablets during the spring camp and placebo tablets during the summer camp. Subjects took these tablets starting 4 weeks before the camp until post-camp saliva sampling. Salivary SIgA concentrations were measured by ELISA. Results All subjects participated in nearly all training programs, and body-mass changes and subjective physical well-being scores during the camps were comparable between the groups. However, salivary SIgA secretion rate changes were different between these groups. Salivary SIgA secretion rates decreased during the camp in the placebo group (before vs. second, middle, and final day of camp, and after the camp: 146 ± 89 vs. 87 ± 56, 70 ± 45, 94 ± 58, and 116 ± 71 μg/min, whereas no such decreases were observed in the chlorella group (121 ± 53 vs. 113 ± 68, 98 ± 69,115 ± 80, and 128 ± 59 μg/min. Conclusion Our results suggest that a use of a chlorella-derived dietary supplement attenuates reduced salivary SIgA secretion during a training camp for a competitive sport.

  10. Camping with the Stars: Queer Perfomativity, Pop Intertextuality, and Camp in the Pop Art of Lady Gaga

    Directory of Open Access Journals (Sweden)

    Horn, Katrin


    Full Text Available The article is concerned with the possibility of employing countercultural and subversive strategies in U.S. mainstream media. The concept in question is camp, historically rooted in gay subculture, as performed by pop artist Lady Gaga. She is presented as challenging gender as well as aesthetic norms in her performances via the employment of camp—thus opening her public persona to queer readings.

  11. Barriers and Facilitators for Generalizing Cycling Skills Learned at Camp to Home. (United States)

    Temple, Viviene A; Purves, P Lynn; Misovic, Robyn; Lewis, Coral J; DeBoer, Carrie


    Many children with disabling conditions do not acquire the skills to successfully ride a 2-wheeled bicycle. The aim was to describe cycling patterns before and after an innovative learn-to-ride bike camp and factors that facilitate or hinder the generalization of skills developed at camp to home. Parents and children participated in semistructured interviews 3-4 mo postcamp. Transcripts were examined deductively for participation and contextual influences using a template of codes approach. None of the children were successfully riding a 2-wheeled bicycle before camp. Two patterns of participation were evident from narrative descriptions of postcamp riding: "riders" and "not there yet." Major facilitating factors were the camp itself, the interaction between the camp and the health service, and continued parent involvement. The program transferred well to home for children who were riding independently on the last day of camp. Ongoing support is needed for children "not there yet."

  12. cAMP biosensors applied in molecular pharmacological studies of G protein-coupled receptors

    DEFF Research Database (Denmark)

    Mathiesen, Jesper Mosolff; Vedel, Line; Bräuner-Osborne, Hans


    end-point assays for quantifying GPCR-mediated changes in intracellular cAMP levels exist. More recently, fluorescence resonance energy transfer (FRET)-based cAMP biosensors that can quantify intracellular cAMP levels in real time have been developed. These FRET-based cAMP biosensors have been used...... primarily in single cell FRET microscopy to monitor and visualize changes in cAMP upon GPCR activation. Here, a similar cAMP biosensor with a more efficient mCerulean/mCitrine FRET pair is described for use in the 384-well plate format. After cloning and expression in HEK293 cells, the biosensor...... is characterized in the 384-well plate format and used for measuring the signaling of the G(s)-coupled ß(2)-adrenergic receptor. The procedures described may be applied for other FRET-based biosensors in terms of characterization and conversion to the 384-well plate format....

  13. How Women Work: The Symbolic and Material Reproduction of Migrant Labor Camps in United States Agribusiness

    Directory of Open Access Journals (Sweden)

    Robert CARLEY


    Full Text Available This article analyzes gender exploitation in Mexican and Central American migrant farm worker camps in the U.S through small group interactions. We describe how gender exploitation and oppression is transmitted through the social fabric of the camp. We argue that the camp produces an endogenous system of social interaction, which maintains uneven gender relationships. Our data is based on observations of twenty-five women and girls in three labor camps in North Carolina. Research was conducted over a period of six weeks. We found that women who served as the primary bearers of patrimonial authority best maintained the camp community. We conclude that women who successfully reproduce the authority structure gain social status in the camps and are more likely to stay.

  14. The Effect of a Nature Camp on Children’s Conceptions of Nature

    Directory of Open Access Journals (Sweden)

    Esra Yardimci


    Full Text Available In this study, a nature camp which provides authentic learning opportunities for children was conducted. Twenty-four 4th and 5th graders (9 girls, 15 boys participated to the camp. The camp program started with observations in the forest nearby. More focused observations were also made. Children discussed their observations with their friends and scientists. A questionnaire was applied at the beginning and end of the camp. The results showed that the nature camp program was effective in extending the children’s conception of the nature by including both living and non-living things. Most of them started the camp with a conception of the nature which consisted of only living things whereas only four children left with this conception at the end. Biological aspects of the nature also increased. Children generally stated that living or non-living things exist or live together at the nature, but relationships between them were noticed by fewer children.

  15. College of Agriculture and Life Sciences will host ExxonMobil Bernard Harris Summer Science Camp


    Greiner, Lori A.


    Virginia Tech has been chosen as a site for a 2007 ExxonMobil Bernard Harris Summer Science Camp on June 18-30. The Virginia Tech camp will be one of 20 hosted by universities across the country. The two-week residential camp offers an innovative program that enhances middle school students' knowledge of science, technology, engineering, and mathematics (STEM) while encouraging students to stay in school and fostering leadership and citizenship.

  16. Bicarbonate-responsive “soluble” adenylyl cyclase defines a nuclear cAMP microdomain (United States)

    Zippin, Jonathan H.; Farrell, Jeanne; Huron, David; Kamenetsky, Margarita; Hess, Kenneth C.; Fischman, Donald A.; Levin, Lonny R.; Buck, Jochen


    Bicarbonate-responsive “soluble” adenylyl cyclase resides, in part, inside the mammalian cell nucleus where it stimulates the activity of nuclear protein kinase A to phosphorylate the cAMP response element binding protein (CREB). The existence of this complete and functional, nuclear-localized cAMP pathway establishes that cAMP signals in intracellular microdomains and identifies an alternate pathway leading to CREB activation. PMID:14769862

  17. Bicarbonate-responsive “soluble” adenylyl cyclase defines a nuclear cAMP microdomain



    Bicarbonate-responsive “soluble” adenylyl cyclase resides, in part, inside the mammalian cell nucleus where it stimulates the activity of nuclear protein kinase A to phosphorylate the cAMP response element binding protein (CREB). The existence of this complete and functional, nuclear-localized cAMP pathway establishes that cAMP signals in intracellular microdomains and identifies an alternate pathway leading to CREB activation.

  18. The effectiveness of an American science camp for Taiwanese high school students (United States)

    Kuo, Pi-Chu

    The purposes of this study were: (1) to evaluate the effectiveness of an American science camp for Taiwanese high school students in terms of student attitudes toward science; (2) to understand the factors that affect student attitudes toward science in the American science camp. Qualitative and quantitative data were collected and analyzed to answer my research questions: (1) How did the influence of the abroad science camp differ from the local one in terms of student attitudes toward science? (2) How did gender, grade level, and personality affect student attitudes toward science in the abroad science camp? An Attitudes toward Science Inventory was used in this study to measure student attitudes. The results of factor analysis suggested that the attitudes measured in this study include five common factors: science as school subjects (SC), science in society (SS), value of science (VS), science in laboratory (SL), and nature of science (NS). Significant improvements were found in SS, VS, and NS after the experiences of the abroad science camp. In the local science camp, only NS was non-significant comparing before and after the camp. The results from the comparisons between the two science camps show that different program designs have different impacts on student attitudes toward science. Furthermore, whether the science camps are designed based on learning theory or not, and regardless of how much time the campers spend in science-related activities during science camps, science camps can motivate students' interests in learning science. The results of mixed-design ANOVA for gender, grade level, and personality suggest that most of these personal factors did not significantly affect student attitudes. However, extraversion/introversion and sensing/intuition had impacts on the persuasibility of the abroad science camp.

  19. Cultured lymphocytes from alcoholic subjects have altered cAMP signal transduction.


    Nagy, L E; Diamond, I; Gordon, A.


    Previous work has shown that freshly isolated lymphocytes from alcoholic subjects show significantly reduced basal and adenosine receptor-stimulated cAMP levels. This decrease could be due to ethanol-induced cellular adaptation or to a genetic difference in the regulation of cAMP signal transduction. Therefore, we cultured human lymphocytes in defined medium without ethanol for 7-8 days and then examined differences in receptor-dependent cAMP accumulation between lymphocytes from alcoholic an...


    Directory of Open Access Journals (Sweden)

    Murad Lesmana


    Full Text Available Suatu modifikasi uji CAMP digunakan bersama dengan reaksi biokimiawi untuk identifikasi Vibrio cholerae pada sampel klinis. Dari 579 usap dubur penderita diare, 92 (16% memberikan hasil isolasi V. cholerae 01 biotipe El Tor dan 34 (6% V. cholerae non-01. Semua isolat V. cholerae 01 El Tor menunjukkan reaksi CAMP positif kuat dengan gambaran hemolisis sinergistik lengkap berbentuk sosis; sedangkan V. cholerae non-01 memberikan reaksi CAMP yang sempit dengan pola hemolisis menyerupai bulan sabit. Hasil uji CAMP yang dilakukan bersama dengan reaksi biokimiawi sesuai dengan metode biakan konvensional yang menyertakan tes aglutinasi dengan antiserum V. cholerae 01 untuk mengidentifikasi V. cholerae.

  1. cAMP diffusion in Dictyostelium discoideum: A Green's function method (United States)

    Calovi, Daniel S.; Brunnet, Leonardo G.; de Almeida, Rita M. C.


    A Green’s function method is developed to approach the spatiotemporal equations describing the cAMP production in Dictyostelium discoideum, markedly reducing numerical calculations times: cAMP concentrations and gradients are calculated just at the amoeba locations. A single set of parameters is capable of reproducing the different observed behaviors, from cAMP synchronization, spiral waves and reaction-diffusion patterns to streaming and mound formation. After aggregation, the emergence of a circular motion of amoebas, breaking the radial cAMP field symmetry, is observed.

  2. "cAMP sponge": a buffer for cyclic adenosine 3', 5'-monophosphate.

    Directory of Open Access Journals (Sweden)

    Konstantinos Lefkimmiatis

    Full Text Available BACKGROUND: While intracellular buffers are widely used to study calcium signaling, no such tool exists for the other major second messenger, cyclic AMP (cAMP. METHODS/PRINCIPAL FINDINGS: Here we describe a genetically encoded buffer for cAMP based on the high-affinity cAMP-binding carboxy-terminus of the regulatory subunit RIbeta of protein kinase A (PKA. Addition of targeting sequences permitted localization of this fragment to the extra-nuclear compartment, while tagging with mCherry allowed quantification of its expression at the single cell level. This construct (named "cAMP sponge" was shown to selectively bind cAMP in vitro. Its expression significantly suppressed agonist-induced cAMP signals and the downstream activation of PKA within the cytosol as measured by FRET-based sensors in single living cells. Point mutations in the cAMP-binding domains of the construct rendered the chimera unable to bind cAMP in vitro or in situ. Cyclic AMP sponge was fruitfully applied to examine feedback regulation of gap junction-mediated transfer of cAMP in epithelial cell couplets. CONCLUSIONS: This newest member of the cAMP toolbox has the potential to reveal unique biological functions of cAMP, including insight into the functional significance of compartmentalized signaling events.


    Cecil, R L; Vaughan, H F


    1. 13,460 men, or about 80 per cent of the entire camp strength, were vaccinated against pneumonia with pneumococcus lipovaccine. 2. The dosage employed in all cases was 1 cc. of the lipovaccine containing approximately 10 billion each of Pneumococcus Types I, II, and III. 3. Both the local and general reactions produced by the vaccine were usually mild. Only 0.7 per cent of those who received the vaccine were sufficiently affected to need hospital care. None of these was seriously ill, and a majority of them returned to duty on the 2nd or 3rd day after admission. 4. Most of the troops inoculated were under observation for 2 or 3 months after vaccination. During this period there were 32 cases of Pneumococcus Type I, II, and III pneumonia among the vaccinated four-fifths of camp, and 42 cases of pneumonia of these types among the unvaccinated one-fifth of camp. If, however, all cases of pneumonia that developed within 1 week after vaccination are excluded from the vaccinated group, there remain only 8 cases of pneumonia produced by fixed types, and these were all secondary to severe attacks of influenza. This exclusion is justified by the fact that protective bodies do not begin to appear in the serum until the 8th day after injection of pneumococcus lipovaccine. 5. There is no evidence whatever that pneumococcus vaccine predisposes the individual even temporarily toward either pneumococcus or streptococcus pneumonia. 6. The weekly incidence rate for pneumonia (all types) among the vaccinated troops was conspicuously lower than that for the unvaccinated troops. 7. The pneumonia incidence rate per 1,000 men during the period of the experiment was twice as high for unvaccinated recruits as for vaccinated recruits, and nearly seven times as high for unvaccinated seasoned men as for vaccinated seasoned men. 8. Influenza causes a marked reduction in resistance to pneumonia even among vaccinated men. Of the 155 cases of pneumonia (all types) developing 1 week or more

  4. Classification of MEC with the ALLTEM at Camp Stanley, Texas (United States)

    Asch, T.; Moulton, C.; Smith, D.V.


    The ALLTEM is a multi-axis electromagnetic induction system designed for unexploded ordnance UXO applications. It uses a continuous triangle-wave excitation and provides good late-time signal-to-noise ratio SNR especially for ferrous targets. Multi-axis transmitter Tx and receiver Rx systems such as ALLTEM provide a richer data set from which to invert for the target parameters required to distinguish between clutter and UXO. Inversions of field data acquired between 2006 and 2010 over the Army's UXO Standardized Test sites at the Yuma Proving Ground YPG in Arizona and at the Aberdeen Proving Ground APG in Maryland have produced reasonable and generally repeatable results for many UXO items buried at different orientations and depths. In February-March 2011 ALLTEM data was acquired at two locations on the Camp Stanley Storage Activity CSSA just north of San Antonio, Texas. Camp Stanley is used to store munitions as well as test, fire, and overhaul munitions components. Site B-20 is an open burn/open detonation OBOD area and Site B-27 consists of narrow trenches blasted into limestone containing buried range and munitions debris and possibly MEC. The processing, analysis, and classification techniques developed at the controlled environments of YPG and APG have been applied to these two "live" sites at Camp Stanley. ALLTEM data analysis includes both classical numerical inversion of data from each anomaly and clustering of the raw data by means of a self-organizing map SOM via generalized neural network algorithms. Final classification consists of an integration of both the numerical and SOM results. ?? 2011 Society of Exploration Geophysicists.

  5. Camp Upshur, Marine Corps Base Quantico, VA Architectural Survey (United States)


    ERDC/CERL SR-09-11 viii Unit Conversion Factors Multiply By To Obtain acres 4,046.873 square meters degrees Fahrenheit (F-32)/1.8 degrees Celsius...Office 1952 No 451 26109 Chapel 1952 No 461 26110 Administrative Office 1952 No 473 26111 Administrative Office 1952 No 483 26112 Storage A/G...Floor Tile Replacement-Camp Upshur, Building 26110, floor plan, March 1962 ERDC/CERL SR-09-11 451 B ui ld in g 26 11 0 HISTORIC PHOTOGRAPHS

  6. The Camp Edwards Experiment in Battalion Level Consolidated Field Feeding (United States)


    Cucumbers 6 - 1.0 1 Apple Juices 8 42 8.5 NATIONAL GUARD CAMP EDWARDS MENU REVISED BY NARADCOM & 101ST EWG 7 J"{JLY -1975 Rl 88 MENU I - SATURDAY 9...August 1975 Dinner Cheeseburgers/Hamburgers (~32) Catsup - Mustard Pickle Relish Lyonnaise Potatoes {Q-54) Buttered Cor~ (Q-G-3) Lettuce and... Pickle Relish O’Brien Potatoes (Q-54) Buttered Green Beans (Q-G-1) Lettuce and Tomato Salad (M-33) Sliced Onion French Dressing (M-58) Hamburger

  7. Genetically-encoded tools for cAMP probing and modulation in living systems.

    Directory of Open Access Journals (Sweden)

    Valeriy M Paramonov


    Full Text Available Intracellular 3'-5'-cyclic adenosine monophosphate (cAMP is one of the principal second messengers downstream of a manifold of signal transduction pathways, including the ones triggered by G protein-coupled receptors. Not surprisingly, biochemical assays for cAMP have been instrumental for basic research and drug discovery for decades, providing insights into cellular physiology and guiding pharmaceutical industry. However, despite impressive track record, the majority of conventional biochemical tools for cAMP probing share the same fundamental shortcoming - all the measurements require sample disruption for cAMP liberation. This common bottleneck, together with inherently low spatial resolution of measurements (as cAMP is typically analyzed in lysates of thousands of cells, underpin the ensuing limitations of the conventional cAMP assays: 1 genuine kinetic measurements of cAMP levels over time in a single given sample are unfeasible; 2 inability to obtain precise information on cAMP spatial distribution and transfer at subcellular levels, let alone the attempts to pinpoint dynamic interactions of cAMP and its effectors. At the same time, tremendous progress in synthetic biology over the recent years culminated in drastic refinement of our toolbox, allowing us not only to bypass the limitations of conventional assays, but to put intracellular cAMP life-span under tight control – something, that seemed scarcely attainable before. In this review article we discuss the main classes of modern genetically-encoded tools tailored for cAMP probing and modulation in living systems. We examine the capabilities and weaknesses of these different tools in the context of their operational characteristics and applicability to various experimental set-ups involving living cells, providing the guidance for rational selection of the best tools for particular needs.

  8. Rapid Assessment of Seismic Vulnerability in Palestinian Refugee Camps (United States)

    Al-Dabbeek, Jalal N.; El-Kelani, Radwan J.

    Studies of historical and recorded earthquakes in Palestine demonstrate that damaging earthquakes are occurring frequently along the Dead Sea Transform: Earthquake of 11 July 1927 (ML 6.2), Earthquake of 11 February 2004 (ML 5.2). In order to reduce seismic vulnerability of buildings, losses in lives, properties and infrastructures, an attempt was made to estimate the percentage of damage degrees and losses at selected refugee camps: Al Ama`ri, Balata and Dhaishe. Assessing the vulnerability classes of building structures was carried out according to the European Macro-Seismic Scale 1998 (EMS-98) and the Fedral Emergency Management Agency (FEMA). The rapid assessment results showed that very heavy structural and non structural damages will occur in the common buildings of the investigated Refugee Camps (many buildings will suffer from damages grades 4 and 5). Bad quality of buildings in terms of design and construction, lack of uniformity, absence of spaces between the building and the limited width of roads will definitely increase the seismic vulnerability under the influence of moderate-strong (M 6-7) earthquakes in the future.

  9. Photovoltaic Technology of Electricity Generation for Desert Camping

    Directory of Open Access Journals (Sweden)

    Shafiqur Rehman


    Full Text Available This study presents a case study on the utilization of global solar radiation data on horizontal surface to perform economical feasibility of using Photovoltaic (PV panels with battery backup to meet a small load for a camping site in Saudi Arabia. The analysis considers three scenarios with daily average energy demands of: (i full load, (ii 75% load and (iii half load with annual peak load of 3.84, 3.06 and 2.27 kW, respectively. Each of these loads is further studied economically to investigate the effect of battery storage for 1 to 5 days. The study also compares the cost of electricity generation in $/kWh from PV system and diesel generating systems. The lower mean temperature (~20°C and high intensity of radiation (~ 6.3 kWh m2/day in Abha make it a promising site for the usage of PV systems for desert camping. Analysis of the data indicates that the battery storage capacity cost plays an important role in the overall cost of the PV system. The economical indicators suggest that larger PV systems be preferred over the smaller ones with minimal storage option. The energy generation cost analysis indicated that the diesel generating cost was found to be 29, 56 and 116% higher than the PV system for full, 75% and half load systems, respectively.

  10. The Efficacy of Family Camp Experience for Families Who Have Children with Visual Impairments. Research Report (United States)

    Day, Janice Neibaur; Kleinschmidt, Julia


    This study was designed to address the paucity of research on the efficacy of camps for children with visual impairments and their families. The study evaluated the performance of a two-day camp for families with young visually impaired children at the Utah Schools for the Deaf and the Blind whose program was based on perceived family needs and…

  11. A Temporal-Specific and Transient cAMP Increase Characterizes Odorant Classical Conditioning (United States)

    Cui, Wen; Smith, Andrew; Darby-King, Andrea; Harley, Carolyn W.; McLean, John H.


    Increases in cyclic adenosine monophosphate (cAMP) are proposed to initiate learning in a wide variety of species. Here, we measure changes in cAMP in the olfactory bulb prior to, during, and following a classically conditioned odor preference trial in rat pups. Measurements were taken up to the point of maximal CREB phosphorylation in olfactory…

  12. Offering a Forensic Science Camp to Introduce and Engage High School Students in Interdisciplinary Science Topics (United States)

    Ahrenkiel, Linda; Worm-Leonhard, Martin


    In this article, we present details of a one-week interdisciplinary science camp for high school students in Denmark, "Criminal Camp". We describe the use of forensic science and simulated crimes as a common foundation for teaching the theory and practice of concepts in chemistry, physics, and medicine or biology. The main goal of the…

  13. The Representation and Appropriation of Indigenous Cultures at Ontario Summer Camps. (United States)

    Hamilton, Ty


    Interviews with directors at five Ontario summer camps found that three camps exposed children to stereotypes of Indigenous peoples and to cultural appropriation. This is inconsistent with goals of educating campers about and showing respect for Indigenous cultures. Given the current issues of land-claims and Aboriginal rights, non-Indigenous…

  14. Alaska Native Elders' Contribution to Education: The Fairbanks AISES Science Camp. (United States)

    Bradley, Claudette; Reyes, Maria Elena

    The Fairbanks American Indian Science and Engineering Society (AISES) Science Camp was designed for Alaska Native middle school students from 11 school districts. The camp enables students to learn from Native Elders while completing hands-on science projects; stimulates interest and confidence in mathematics, science, and engineering among Alaska…

  15. Hands-on Summer Camp to Attract K-12 Students to Engineering Fields (United States)

    Yilmaz, Muhittin; Ren, Jianhong; Custer, Sheryl; Coleman, Joyce


    This paper explains the organization and execution of a summer engineering outreach camp designed to attract and motivate high school students as well as increase their awareness of various engineering fields. The camp curriculum included hands-on, competitive design-oriented engineering projects from several disciplines: the electrical,…

  16. Adventure, Wilderness, Outward Bound, Therapeutic Camping, Experiental Learning, Ropes Courses & Games. (United States)

    Gillis, H. Lee

    Focusing on the therapeutic aspects of camping experiences for delinquent and psychologically disturbed youth, this 382-item bibliography lists books, journal articles, theses, dissertations, and unpublished reports written from 1928 to 1983 on adventure education, wilderness experience, Outward Bound, therapeutic camping, experiential learning,…

  17. A novel conditional genetic system reveals that increasing neuronal cAMP enhances memory and retrieval

    NARCIS (Netherlands)

    Isiegas, Carolina; McDonough, Conor; Huang, Ted; Havekes, Robbert; Fabian, Sara; Wu, Long-Jun; Xu, Hui; Zhao, Ming-Gao; Kim, Jae-Ick; Lee, Yong-Seok; Lee, Hye-Ryeon; Ko, Hyoung-Gon; Lee, Nuribalhae; Choi, Sun-Lim; Lee, Jeong-Sik; Son, Hyeon; Zhuo, Min; Kaang, Bong-Kiun; Abel, Ted


    Consistent evidence from pharmacological and genetic studies shows that cAMP is a critical modulator of synaptic plasticity and memory formation. However, the potential of the cAMP signaling pathway as a target for memory enhancement remains unclear because of contradictory findings from pharmacolog

  18. Student-Designed Mapping Project as Part of a Geology Field Camp (United States)

    Kelley, Daniel F.; Sumrall, Jeanne L.; Sumrall, Jonathan B.


    During the summer of 2012, the Louisiana State University (LSU) field camp program was affected by close proximity to the large Waldo Canyon Fire in Colorado Springs, CO, as well as by a fire incident on the field camp property. A mapping exercise was created that incorporated spatial data acquired on the LSU property to investigate research…

  19. Targeting brain tumor cAMP: the case for sex-specific therapeutics

    Directory of Open Access Journals (Sweden)

    Nicole M Warrington


    Full Text Available A relationship between cyclic adenosine 3’, 5’-monophosphate (cAMP levels and brain tumor biology has been evident for nearly as long as cAMP and its synthetase, adenylate cyclase (ADCY have been known. The importance of the pathway in brain tumorigenesis has been demonstrated in vitro and in multiple animal models. Recently, we provided human validation for a cooperating oncogenic role for cAMP in brain tumorigenesis when we found that SNPs in ADCY8 were correlated with glioma (brain tumor risk in individuals with Neurofibromatosis type 1 (NF1. Together, these studies provide a strong rationale for targeting cAMP in brain tumor therapy. However, the cAMP pathway is well known to be sexually dimorphic, and SNPs in ADCY8 affected glioma risk in a sex-specific fashion, elevating the risk for females while protecting males. The cAMP pathway can be targeted at multiple levels in the regulation of its synthesis and degradation. Sex differences in response to drugs that target cAMP regulators indicate that successful targeting of the cAMP pathway for brain tumor patients is likely to require matching specific mechanisms of drug action with patient sex.

  20. The Influence of Science Summer Camp on African-American High School Students' Career Choices (United States)

    Bhattacharyya, Sumita; Mead, Timothy P.; Nathaniel, Rajkumar


    This study explored if a weeklong science camp changed Louisiana African-American high school students' perception of science. A semi-structured survey was used before and after the camp to determine the changes in science attitudes and career choices. Among the perceived benefits were parental involvement, increased science academic ability, and…

  1. 14 CFR 91.1437 - CAMP: Authority to perform and approve maintenance. (United States)


    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false CAMP: Authority to perform and approve maintenance. 91.1437 Section 91.1437 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Ownership Operations Program Management § 91.1437 CAMP: Authority to perform and approve maintenance....

  2. AIDS Knowledge among Latinos: Findings from a Community and Agricultural Labor Camp Survey. (United States)

    Urizar, Guido G., Jr.; Winkleby, Marilyn A.


    A study examining AIDS awareness among northern California Latinos surveyed 817 Latinos from a community and 188 Latino men from migrant labor camps. Misconceptions about AIDS transmission were highest among Latinos with low educational attainment, particularly men from labor camps, older Latinos, and Latinos with low educational attainment who…

  3. Global and local missions of cAMP signaling in neural plasticity, learning and memory

    Directory of Open Access Journals (Sweden)

    Daewoo eLee


    Full Text Available The fruit fly Drosophila melanogaster has been a popular model to study cAMP signaling and resultant behaviors due to its powerful genetic approaches. All molecular components (AC, PDE, PKA, CREB, etc essential for cAMP signaling have been identified in the fly. Among them, adenylyl cyclase (AC gene rutabaga and phosphodiesterase (PDE gene dunce have been intensively studied to understand the role of cAMP signaling. Interestingly, these two mutant genes were originally identified on the basis of associative learning deficits. This commentary summarizes findings on the role of cAMP in Drosophila neuronal excitability, synaptic plasticity and memory. It mainly focuses on two distinct mechanisms (global versus local regulating excitatory and inhibitory synaptic plasticity related to cAMP homeostasis. This dual regulatory role of cAMP is to increase the strength of excitatory neural circuits on one hand, but to act locally on postsynaptic GABA receptors to decrease inhibitory synaptic plasticity on the other. Thus the action of cAMP could result in a global increase in the neural circuit excitability and memory. Implications of this cAMP signaling related to drug discovery for neural diseases are also described.

  4. Assessment of the Psychosocial Development of Children Attending Nursery Schools in Karen Refugee Camps in Thailand (United States)

    Tanaka, Akiko


    The Karen, an ethnic minority group in Burma, have experienced a prolonged state of exile in refugee camps in neighboring Thailand because of ethnic conflict in their home country. Nursery schools in the three largest Karen refugee camps aim to promote the psychosocial development of young children by providing a child-centered, creative,…

  5. The Cyclic Nucleotide Specificity of Three cAMP Receptors in Dictyostelium

    NARCIS (Netherlands)

    Johnson, Ronald L.; Haastert, Peter J.M. van; Kimmel, Alan R.; Saxe III, Charles L.; Jastorff, Bernd; Devreotes, Peter N.


    cAMP receptors mediate signal transduction pathways during development in Dictyostelium. A cAMP receptor (cAR1) has been cloned and sequenced (Klein, P., Sun, T. J., Saxe, C. L., Kimmel, A. R., Johnson, R. L., and Devreotes, P. N. (1988) Science 241, 1467-1472) and recently several other cAR genes h

  6. Neuroeconomics: Two camps gradually converging: What can economics gain from it?

    NARCIS (Netherlands)

    J.J. Vromen (Jack)


    textabstractNeuroeconomics started off as a hybrid project. Two camps, behavioral economics in the scanner (BES) and Glimcher's economics of neural activity (ENA), could be clearly distinguished. The camps disagreed not only about substantive issues but also about what neuroeconomics ultimately aims

  7. Which Social Emotional Competencies Are Enhanced at a Social Emotional Learning Camp? (United States)

    Ee, Jessie; Ong, Chew Wei


    Research studies have shown that educational programmes such as camps and field trips can develop affective and social relationships through personal exposure to outdoor experiences among students. This study will illustrate the outcome of a social emotional learning camp organized for 93 Secondary Two students (mean age 14.1) in Singapore. Both…

  8. Effects of a Conservation Education Camp Program on Campers' Self-Reported Knowledge, Attitude, and Behavior (United States)

    Kruse, Cara K.; Card, Jaclyn A.


    In this study, the authors examined the effects of a conservation education camp program offered through one zoo education department. The conservation education program included 4 levels of camps with increasing levels of animal husbandry. Campers rated their conservation knowledge, attitude, and behavior prior to, immediately after, and 1 month…

  9. Educational Camps and Their Effects on Female Perceptions of Technology Programs (United States)

    Jacobs-Rose, Christina; Harris, Kara


    The purpose of this study was to investigate the impact of Cheering in the Classroom, a four day program designed purposefully to increase female awareness and perceptions of technology fields. The camp included discipline-based activities from the 13 different programs of study based in technology. The camp targeted high school cheerleaders and…

  10. The modulation of cell surface cAMP receptors from Dictyostelium disscoideum by ammonium sulfate

    NARCIS (Netherlands)

    Haastert, Peter J.M. van


    Dictyostelium discoideum cells contain a heterogeneous population of cell surface cAMP receptors with components possessing different affinities (Kd between 15 and 450 nM) and different off-rates of the cAMP-receptor complex (t½ between 0.7 and 150 s). The association of cAMP to the receptor and the

  11. An Evaluation of Hope Following a Summer Camp for Inner-City Youth (United States)

    Kirschman, Keri J.; Roberts, Michael C.; Shadlow, Joanna O.; Pelley, Terri J.


    This study reports changes in the positive psychology construct of hope resulting from adolescents' participation in a 6 week summer camp devoted to developing dance and psychosocial competence skills. Over 5 years, the inner-city camp participants were selected from substantial at-risk situations. Significant positive changes in overall hope were…

  12. Distancing Students from Nature: Science Camp and the Representation of the Human-Nature Relationship (United States)

    Terrill, Laura Anne


    This study investigated the curricular representations of the environment and the human-environment relationship at one residential school sponsored science camp. Data gathered included field notes from observational time at the camp, interviews with staff and classroom teachers, and documents from the site's website, guides, manuals, and…

  13. The Efficacy of Mammography Boot Camp to Improve the Performance of Radiologists

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Eun Hye [Dept. of Radiology, Bucheon Hospital, Soonchunhyang University College of Medicine, Bucheon (Korea, Republic of); Jun, Jae Kwan [National Cancer Control Institute, National Cancer Center, Goyang (Korea, Republic of); Jung, Seung Eun [Dept. of Radiology, Seoul St. Mary' s Hospital, College of Medicine, The Catholic University of Korea, Seoul (Korea, Republic of); Kim, You Me [Dept. of Radiology, Dankook University Hospital, Cheonan (Korea, Republic of); Choi, Nami [Dept. of Radiology, Konkuk University Medical Center, Seoul (Korea, Republic of)


    To evaluate the efficacy of a mammography boot camp (MBC) to improve radiologists' performance in interpreting mammograms in the National Cancer Screening Program (NCSP) in Korea. Between January and July of 2013, 141 radiologists were invited to a 3-day educational program composed of lectures and group practice readings using 250 digital mammography cases. The radiologists' performance in interpreting mammograms were evaluated using a pre- and post-camp test set of 25 cases validated prior to the camp by experienced breast radiologists. Factors affecting the radiologists' performance, including age, type of attending institution, and type of test set cases, were analyzed. The average scores of the pre- and post-camp tests were 56.0 ± 12.2 and 78.3 ± 9.2, respectively (p < 0.001). The post-camp test scores were higher than the pre-camp test scores for all age groups and all types of attending institutions (p < 0.001). The rate of incorrect answers in the post-camp test decreased compared to the pre-camp test for all suspicious cases, but not for negative cases (p > 0.05). The MBC improves radiologists' performance in interpreting mammograms irrespective of age and type of attending institution. Improved interpretation is observed for suspicious cases, but not for negative cases.

  14. The effect of neuropeptides on vessel tone and cAMP production

    DEFF Research Database (Denmark)

    Yao, W; Sheikh, S P; Ottesen, B;


    The effect of VIP, PHM, PHV, PACAP-27, and PACAP-38 on vessel tone and cAMP production was investigated in rabbit ovarian arteries in vitro. The peptides (10(-7)M) induced a significant relaxation on NA-precontracted vessels and displayed similar potencies. The cAMP accumulation induced by PACAP-...

  15. Alexander Pechersky Testifies: an Open Page of Sobibor Death Camp History

    Directory of Open Access Journals (Sweden)

    Lev S. Simkin


    Full Text Available Here, the author introduces the interrogation of the witness Alexander Aronovich Pechersky, the leader of the German death camp Sobibor Revolt during the World War II. Special attention is attached to the daily life of the death camp. The picture of revolt preparation was completed

  16. Les novel·les dels camps de concentració

    Directory of Open Access Journals (Sweden)

    Vicent Simbor Roig


    novelistic fiction, the elaboration of a rather peculiar conception of time and space that makes up the true chronotope of the concentration camp, narrative options with regard to the narrator, etc. The final conclusion is that the testimonial novel describing life in a concentration camp is a model with very clear characteristics.

  17. The progressive nature of concentration camp syndrome in former prisoners of Nazi concentration camps--Not just history, but the important issue of contemporary medicine. (United States)

    Jabłoński, Robert; Rosińczuk, Joanna; Leszek, Jerzy; Uchmanowicz, Izabella; Panaszek, Bernard


    Constant stress, slave labor, tortures, and starvation all affected the health of concentration camp prisoners, contributing to multimorbidities, increased mortality and accelerated development of chronic illnesses, what we have shown in an earlier publication. The interrelated somatic and psychological symptoms gave rise to concentration camp syndrome (KZ-syndrome), which has many features of PTSD, occurring frequently nowadays. The paper attempts at assessing the influence of concentration camp conditions on functional disorders in each system of the human body, occurring in KZ-syndrome, and at demonstrating the progressive nature of the syndrome. A retrospective assessment of the former prisoners' health after 5 and 30 years following their leaving camps was performed based on medical records and surveys. The materials included 250 former prisoners who underwent medical examination in 1950, i.e. 5 years after leaving the camp, of whom 120 former prisoners survived and were examined and surveyed in 1975, i.e. 30 years after leaving the camp. KZ-syndrome was shown to occur in 58.8% of former prisoners 5 years after leaving the camp, and in 77.5% after 30 years (p < 0.001), which confirms the syndrome's chronic and progressive nature. Pathological sequels of internment in concentration camps, in the form of KZ-syndrome, were observed in most former prisoners. Over time, the number of morbidities and the intensity of symptoms increased, which indicates that the syndrome has a chronic and progressive nature. KZ-syndrome is a multi-organ disorder, with numerous chronic comorbidities exacerbating the progression.

  18. cAMP Signals in Drosophila Motor Neurons Are Confined to Single Synaptic Boutons

    Directory of Open Access Journals (Sweden)

    Isabella Maiellaro


    Full Text Available The second messenger cyclic AMP (cAMP plays an important role in synaptic plasticity. Although there is evidence for local control of synaptic transmission and plasticity, it is less clear whether a similar spatial confinement of cAMP signaling exists. Here, we suggest a possible biophysical basis for the site-specific regulation of synaptic plasticity by cAMP, a highly diffusible small molecule that transforms the physiology of synapses in a local and specific manner. By exploiting the octopaminergic system of Drosophila, which mediates structural synaptic plasticity via a cAMP-dependent pathway, we demonstrate the existence of local cAMP signaling compartments of micrometer dimensions within single motor neurons. In addition, we provide evidence that heterogeneous octopamine receptor localization, coupled with local differences in phosphodiesterase activity, underlies the observed differences in cAMP signaling in the axon, cell body, and boutons.

  19. Free will in total institutions: The case of choice inside Nazi death camps. (United States)

    Davidov, Jonathan; Eisikovits, Zvi


    Nazi death camps, as any total institutions, were designed to deny any free will or choice from inmates. Furthermore, former inmates in such extreme conditions often account for their own actions and behavior in such settings as inevitable ("I had no other choice"). This study examines the questions of free will vs. determinism in death camps from a descriptive-phenomenological perspective. Data was collected through in-depth interviews with 20 former death camp inmates. The following themes emerged from the qualitative analysis of the data: the 'selection' experience; 'borrowed time' perception; and the experience of 'nothingness'. A conceptual model grounded in these data was developed to illustrate the inmate's lived experience of choice in the reality of the camps. Analysis of the model indicates that under the extreme conditions of the death camp, free will and existence are interchangeable: "I choose - therefore I am".

  20. Evaluation of undergraduate nursing students' clinical confidence following a mental health recovery camp. (United States)

    Cowley, Thomas; Sumskis, Sue; Moxham, Lorna; Taylor, Ellie; Brighton, Renee; Patterson, Chris; Halcomb, Elizabeth


    In the present study, we evaluate the impact of participation in a mental health recovery camp on the clinical confidence of undergraduate nursing students in dealing with individuals with mental illness. Twenty undergraduate nursing students who participated in the recovery camp completed the Mental Health Nursing Clinical Confidence Scale both before and directly after attending the camp. Data were analysed using descriptive and inferential statistics. Participation in the recovery camp was associated with a statistically-significant increase in students' level of overall confidence between the pretest and post-test data (P students over the age of 25 years and who do not have a family history of mental illness are more likely to self-report a higher level of confidence in both the pre- and post-results. The clinical confidence of undergraduate nursing students improved through participation in an immersive clinical experience within the recovery camp.

  1. Agitation and Propagandistic Work in Soviet POW Camps

    Directory of Open Access Journals (Sweden)

    Gulzhaukhar K. Kokebayeva


    Full Text Available The paper studies the problem of agitation work done among POWs in Soviet camps, the creation of military units and political organizations from POWs. Not only armed force was used during the Second World War, but also the power of words. The battles were accompanied by the information warfare. Opponents tried to use all possible means to manipulate people’s minds. Main directions of agitation and propaganda were defined by the «Soviet bureau of military and political propaganda», as well as the 7th Division of Soviet army. In the propaganda work among German POWs, the priority was given on shaping the ideological and political views of former soldiers and officers of the Wehrmacht. As the result of the analysis of sources the author comes to conclusion that POWs of the Second World War period became the object of testing means and methods of ideological struggle of warring nations.

  2. A test of stress theory: relief workers in refugee camps. (United States)

    Soliman, Hussein H; Gillespie, David F


    The purpose of this paper is to apply a stress model drawn from the literature to the relief and social service workers who have been active in refugee camps for a prolonged period of time. Working in difficult environments, social service workers deliver essential services to refugee populations around the world. A model of four work-stress determinants--tasks, management, appreciation and collaboration--was tested on 274 social workers in five regions of the Middle East (Jordan, Lebanon and Syria, as well as the occupied Palestinian territories of the Gaza Strip and the West Bank). Statistical fit indices were adequate but two relationships were statistically insignificant. The collaboration variable was dropped to create a modified model with tasks indirectly and management and appreciation directly affecting work-related stress. The five direct relationships and two indirect relationships of this modified model are consistent with stress theory, and all relationships--direct and indirect--are statistically significant.

  3. UAV Survey Data from Clifton Camp (ST56557330, Bristol, UK

    Directory of Open Access Journals (Sweden)

    Stephen Gray


    Full Text Available This data was collected via low-altitude UAV (Unmanned Aerial Vehicle survey of an area of Clifton Camp (ST565557330, best known for its Iron Age promontory fort. The dataset comprises of metadata records, near-vertical photographs and a derived 3D polygonal mesh. This dataset has been constructed with two kinds of reuse in mind: Firstly, the area surveyed is culturally rich and underexplored; while some of the non-natural features detected by this survey can be identified, others cannot. This data is intended to inform future investigations of the site. Secondly, the survey methodologies employed and the structuring of the resulting dataset are intended to act as an exemplar, a standard method of creating survey data while prioritising open technologies, and of organising UAV survey datasets to ensure maximum re-usability.

  4. Crisis DSM Generation To Support Refugee Camp Management (United States)

    Gstaiger, Veronika; d'Angelo, Pablo; Schneiderhan, Tobais; Krauss, Thomas


    The extraction of high resolution surface information from satellite data has become an important area of research. One of the numerous fields of application is disaster management. Detailed information about the affected terrain is not only needed for analyses during the emergency relief phase, but also for reconstruction and prevention activities. In this paper the authors present the generation of a Digital Surface Model (DSM) based on three very high resolution optical satellite images. The DSM was produced to supplement a flood mapping activity in Jordan and serves as example for the implementation of scientific results during an emergency request. The flood affected the Zaatari refugee camp in Jordan and was mapped by the Center for Satellite Based Crisis Information (ZKI) at the German Aerospace Center (DLR) in January 2013 under emergency mapping conditions.

  5. The psychological and social impact of camp for children with chronic illnesses: a systematic review update. (United States)

    Moola, F J; Faulkner, G E J; White, L; Kirsh, J A


    Advances in medicine have reduced mortality among children with complex medical conditions, resulting in a growing number of young patients living with chronic illnesses. Despite an improved prognosis, these children experience significant psychosocial morbidity, such as depression and anxiety. Therapeutic summer recreation camps have been proposed as an intervention to enhance quality of life among these children. The purpose of this systematic review was to assess the psychosocial impact of camp for children with chronic illnesses. A systematic review of central databases was undertaken using key words, and a rating tool – the Effective Public Health Practice Project (EPHPP) Quality Assessment Tool for Quantitative Studies – was employed to rate methodological quality. 21 studies were included in this systematic review. Although overall methodological quality was weak, camp participation appeared to offer short-term psychosocial benefits on some parameters in children with a variety of chronic illnesses. There was some consistency in improved social outcomes, such as social interaction and acceptance. Based on the available evidence, it is premature to make robust claims regarding the psychosocial impact of camp as a therapeutic intervention. Theoretically informed camp programs, long-term follow-up, and incorporating camp-based messaging into routine hospital care,may enhance the utility of camp as a potential psychosocial intervention in paediatrics.

  6. Suicide in Inmates in Nazis and Soviet Concentration Camps: Historical Overview and Critique (United States)

    López-Muñoz, Francisco; Cuerda-Galindo, Esther


    Living conditions in concentration camps were harsh and often inhumane, leading many prisoners to commit suicide. We have reviewed this topic in Nazi concentration camps (KL), Soviet special camps, and gulags, providing some preliminary data for our research. Data show that the incidence of suicide in Nazi KL could be up to 30 times higher than the general population and was also much higher than in Soviet special camps (maybe due to more favorable conditions for prisoners and the abolishment of death penalty), while available data on Soviet gulags are contradictory. However, data interpretation is very controversial, because, for example, the Nazi KL authorities used to cover-up the murder victims as suicides. Most of the suicides were committed in the first years of imprisonment, and the method of suicide most commonly used was hanging, although other methods included cutting blood vessels, poisoning, contact with electrified wire, or starvation. It is possible to differentiate two behaviors when committing suicide; impulsive behavior (contact with electrified barbed wire fences) or premeditated suicide (hanging up or through poison). In Soviet special camps, possible motives for suicides could include feelings of guilt for crimes committed, fear of punishment, and a misguided understanding of honor on the eve of criminal trials. Self-destructive behaviors, such as self-mutilation in gulag camps or prisoners who let themselves die, have been widely reported. Committing suicide in concentration camps was a common practice, although precise data may be impossible to obtain. PMID:27303312

  7. cAMP Modulates Macrophage Development by Suppressing M-CSF-Induced MAPKs Activation

    Institute of Scientific and Technical Information of China (English)

    Ning Zhu; Jian Cui; Chunxia Qiao; Yan Li; Yuanfang Ma; Jiyan Zhang; Beifen Shen


    M-CSF is a key cytokine in macrophage development by inducing MAPKs activation, and cAMP can inhibit MAPKs activation induced by inflammatory stimuli. To explore the effects of cAMP on M-CSF-induced MAPKs activation and on macrophage development, the model of bone marrow-derived murine macrophages (BMMs) was used. The effects of cAMP on M-CSF-induced MAPKs activation were analyzed by Western blotting assay, and the effects of cAMP on CD14 and F4/80 expression during macrophage development were examined by FACS analysis.Macrophage morphology showed the successful establishment of the model of macrophage development. Western blotting assay revealed that M-CSF activated ERK, JNK and p38 in both mature and immature macrophages, and cAMP inhibited M-CSF-induced ERK, JNK and p38 activation in a time-dependent manner. FACS analysis revealed that macrophage development was impaired with cAMP pretreatment. In conclusion, cAMP modulates macrophage development by suppressing M-CSF-induced MAPKs activation.

  8. The first camps in Turkey for asthmatic children: six years' experience. (United States)

    Oneş, Ulker; Sapan, Nihat; Yazicioğlu, Mehtap; Güler, Nermin; Tamay, Zeynep; Somer, Ayper; Canitez, Yakup


    The first asthma camp in Turkey was organized for one week in Iznik in September 1996. The camps were continued annually around the same time of the year in 1997, 1998, 2000, and in the consecutive years thereafter. The camp includes educational, sports and social activities. Children's knowledge about asthma and their attitudes towards physical and social activities were evaluated by a questionnaire. Pulmonary function tests were performed on the first and last day of the camp. There was no statistically significant difference between the mean spirometric values of the first and last day of the camp in spite of a vigorous physical and social program. Children enhanced their skills and knowledge about asthma and gained self-confidence in participating in sportive and social activities during the camp program. They did not experience any emergency room visit in the following year. In conclusion, a summer camping experience is very beneficial for asthmatic children in terms of both self-education and social and physical participation.

  9. Suicide in inmates in Nazis and Soviet concentration camps: historical overview and critique

    Directory of Open Access Journals (Sweden)

    Francisco eLopez-Munoz


    Full Text Available Living conditions in concentration camps were harsh and often inhumane, leading many prisoners to commit suicide. We have reviewed this topic in Nazi concentration camps (KL, Soviet special camps and gulags, providing some preliminary data of our research. Data show that the incidence of suicide in Nazi KL could be up to 30 times higher than the general population, and was also much higher than in Soviet special camps (maybe due to more favorable conditions for prisoners and the abolishment of death penalty, while available data on Soviet gulags are contradictory. However, data interpretation is very controversial, because, for example, the Nazi KL authorities used to cover up the murder victims as suicides. Most of suicides were committed in the first years of imprisonment and the method of suicide most commonly used was hanging, although other methods included cutting blood vessels, poisoning, contact with electrified wire, or starvation. It is possible to differentiate two behavior when committing suicide; impulsive behavior (contact with electrified barbed or premeditated suicide (hanging up or through poison. In Soviet special camps, possible motives for suicides could include feelings of guilt for crimes committed, fear of punishment and a misguided understanding of honor on the eve of criminal trials. Self-destructive behaviors such as self-mutilation in gulag camps or prisoners who let themselves die have been widely reported. Committing suicide in concentration camps was a common practice, although precise data may be impossible to obtain.

  10. Responsiveness to Exogenous Camp of a Saccharomyces Cerevisiae Strain Conferred by Naturally Occurring Alleles of Pde1 and Pde2


    Mitsuzawa, H.


    The Saccharomyces cerevisiae strain P-28-24C, from which cAMP requiring mutants derived, responded to exogenously added cAMP. Upon the addition of cAMP, this strain showed phenotypes shared by mutants with elevated activity of the cAMP pathway. Genetic analysis involving serial crosses of this strain to a strain with another genetic background revealed that the responsiveness to cAMP results from naturally occurring loss-of-function alleles of PDE1 and PDE2, which encode low and high affinity...

  11. The role of cAMP in nerve growth factor-promoted neurite outgrowth in PC12 cells



    Nerve growth factor (NGF)-mediated neurite outgrowth in rat pheochromocytoma PC12 cells has been described to be synergistically potentiated by the simultaneous addition of dibutyryl cAMP. To elucidate further the role of cAMP in NGF-induced neurite outgrowth we have used the adenylate cyclase activator forskolin, cAMP, and a set of chemically modified cAMP analogues, including the adenosine cyclic 3',5'-phosphorothioates (cAMPS) (Rp)-cAMPS and (Sp)-cAMPS. These diastereomers have differentia...

  12. Role of cAMP in the reactivation of demembranated ram spermatozoa. (United States)

    San Agustin, J T; Witman, G B


    Ejaculated ram sperm were demembranated with Triton X-100, separated from the detergent-soluble matrix, and reactivated [San Agustin and Witman (1993): Cell Motil. Cytoskeleton 24:264-273]. The percent motility of models prepared from freshly washed sperm was comparable to that of the washed sample before demembranation, regardless of whether cAMP was included in the reactivation medium. However, demembranated models derived from aging or metabolically inhibited sperm exhibited a lower percent reactivation and required cAMP to attain the level of motility of freshly washed sperm. Cyclic AMP was approximately 100 times more effective than cGMP. The requirement for cAMP could be bypassed by addition of porcine heart cAMP-dependent protein kinase (PKA) catalytic subunit to the reactivation medium, demonstrating that cAMP was acting via PKA. The cAMP stimulation of reactivation was not affected by inclusion of the PKA inhibitor PKI(5-24) in the reactivation medium, but was decreased when the models were preincubated with PKI(5-24) prior to reactivation. The cytosol-free models retained > 90% of the sperm PKA activity; therefore, the PKA appears to be anchored to internal sperm structures. This PKA could not be extracted by cAMP or Triton X-100 alone, but only by cAMP and Triton X-100 in combination. We conclude that cAMP-dependent protein phosphorylation is critical for sperm motility, but that the essential protein phosphate sites turn over slowly under our reactivation conditions, so that the cAMP requirement is apparent only in models prepared from sperm having a low internal ATP or cAMP content. Interestingly, reactivation was rapidly blocked by the peptide arg-lys-arg-ala-arg-lys-glu, which has been reported to be a selective inhibitor of cGMP-dependent protein kinase.

  13. Between life and death: experiences of concentration camp mussulmen during the holocaust. (United States)

    Ryn, Z


    During the years of 1981 and 1982, 89 former prisoners of the Auschwitz-Birkenau concentration camp responded to questionnaires on mussulmen-prisoners in the extreme phase of starvation disease. In this article, I describe the origin of the term "mussulman," mussulmens' somatic and mental state, their behavior and camp customs. Prisoners characterized as mussulmen remain between life and death, without expressing emotional reactions and defense mechanisms apart from a hypersensibility to food-related stimuli. A mussulman was a product of the camp factory of death. A deep somatic and emotional stigma remains in those who survived the mussulmen state.

  14. Comparison of cAMP with other radioprotectors against chronic damage to the rat parotid gland

    Energy Technology Data Exchange (ETDEWEB)

    Conger, A.D.; Sodicoff, M.; Samel, A.


    Radiation damage to the parotid gland is protectable by cAMP during the first week after irradiation (acute phase), though appreciable recovery occurred later with or without such protection. Further damage developed later (chronic phase, 60-90 days), and cAMP was still protective against this damage with a dose modification factor of 1.86 for gland weight. A summary of the protective factors, acute and chronic, for WR-2721, isoproterenol, and cAMP is included. Chronic damage is about 1.5 times as great as acute, and protection against acute and chronic damage is about equal for all three compounds.

  15. cAMP prevents TNF-induced apoptosis through inhibiting DISC complex formation in rat hepatocytes

    Energy Technology Data Exchange (ETDEWEB)

    Bhattacharjee, Rajesh [Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA 15213 (United States); Xiang, Wenpei [Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA 15213 (United States); Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People' s Republic of China (China); Wang, Yinna [Vascular Medicine Institute, University of Pittsburgh School of Medicine, 10051-5A BST 3, 3501 Fifth Avenue, Pittsburgh, PA 15261 (United States); Zhang, Xiaoying [Department of Medicine/Endocrinology Division, University of Pittsburgh Medical Center, 200 Lothrop St., Pittsburgh, PA 15213 (United States); Billiar, Timothy R., E-mail: [Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA 15213 (United States)


    Highlights: Black-Right-Pointing-Pointer cAMP blocks cell death induced by TNF and actinomycin D in cultured hepatocytes. Black-Right-Pointing-Pointer cAMP blocks NF-{kappa}B activation induced by TNF and actinomycin D. Black-Right-Pointing-Pointer cAMP blocks DISC formation following TNF and actinomycin D exposure. Black-Right-Pointing-Pointer cAMP blocks TNF signaling at a proximal step. -- Abstract: Tumor necrosis factor {alpha} (TNF) is a pleiotropic proinflammatory cytokine that plays a role in immunity and the control of cell proliferation, cell differentiation, and apoptosis. The pleiotropic nature of TNF is due to the formation of different signaling complexes upon the binding of TNF to its receptor, TNF receptor type 1 (TNFR1). TNF induces apoptosis in various mammalian cells when the cells are co-treated with a transcription inhibitor like actinomycin D (ActD). When TNFR1 is activated, it recruits an adaptor protein, TNF receptor-associated protein with death domain (TRADD), through its cytoplasmic death effector domain (DED). TRADD, in turn, recruits other signaling proteins, including TNF receptor-associated protein 2 (TRAF2) and receptor-associated protein kinase (RIPK) 1, to form a complex. Subsequently, this complex combines with FADD and procaspase-8, converts into a death-inducing signaling complex (DISC) to induce apoptosis. Cyclic AMP (cAMP) is a second messenger that regulates various cellular processes such as cell proliferation, gene expression, and apoptosis. cAMP analogues are reported to act as anti-apoptotic agents in various cell types, including hepatocytes. We found that a cAMP analogue, dibutyryl cAMP (db-cAMP), inhibits TNF + ActD-induced apoptosis in rat hepatocytes. The protein kinase A (PKA) inhibitor KT-5720 reverses this inhibitory effect of cAMP on apoptosis. Cytoprotection by cAMP involves down-regulation of various apoptotic signal regulators like TRADD and FADD and inhibition of caspase-8 and caspase-3 cleavage. We also found

  16. 1992 Environmental Summer Science Camp Program evaluation. The International Environmental Institute of Westinghouse Hanford Company

    Energy Technology Data Exchange (ETDEWEB)


    This report describes the 1992 Westinghouse Hanford Company/US Department of Energy Environmental Summer Science Camp. The objective of the ``camp`` was to motivate sixth and seventh graders to pursue studies in math, science, and the environment. This objective was accomplished through hands-on fun activities while studying the present and future challenges facing our environment. The camp was funded through Technical Task Plan, 424203, from the US Department of Energy-Headquarters, Office of Environmental Restoration and Waste Management, Technology Development,to Westinghouse Hanford Company`s International Environmental Institute, Education and Internship Performance Group.

  17. Exogenous Camp upregulates the expression of glnII and glnK-amtB genes in Sinorhizobium meliloti 1021

    Institute of Scientific and Technical Information of China (English)

    TIAN Zhexian; MAO Xianjun; SU Wei; LI Jian; BECKER Anke; WANG Yiping


    The existence of multiple adenylate cyclase encoding genes implies the importance of Camp in Sinorhizobium meliloti 1021. In this study, as a pioneer step of understanding Camp roles, microarray analysis on S. Meliloti was carried out for the function of exogenous Camp. To our surprise, the result showed that the transcriptions of glnII and glnK genes were significantly upshifted in the presence of exogenous Camp in S. Meliloti. This phenomenon is further confirmed in S. Meliloti that the expression of either glnII or glnK promoter-lacZ translational fusion is higher in the presence of exogenous Camp.Therefore, for the first time, we have identified genes from S. Meliloti whose expression is activated by Camp. The potential physiological role of upregulation of glnII and glnK by Camp is discussed.

  18. A Residential Summer Camp--A Vehicle for Promoting Daily Living Skills. (United States)

    Maron, Sheldon; Hassler, Therese


    In an eight-week residential summer camp program for visually impaired children, activities of daily living (ADL) were offered as part of the regular program. Campers consistently indicated that this was a most worthwhile learning experience. (CL)

  19. From Death To Death:Some Thoughts about Indian Camp by Ernest Hemingway

    Institute of Scientific and Technical Information of China (English)

    LI Hui


    Based on Indian Camp, the thesis probes into the death complex of Ernest Hemingway from the visual angle of Nick, the protagonist of the novel, death complex shown in Hemingway’s works and Nick, the archetype of Hemingway respectively.

  20. Malheur National Wildlife Refuge: Narrative Report: 1938: Camp Buena Vista BF-3: 3: July-August (United States)

    US Fish and Wildlife Service, Department of the Interior — This narrative report from the Civilian Conservation Corps summarizes activities done on Camp Buena Vista. Topics include land development and maintenance, wildlife...

  1. Malheur National Wildlife Refuge: Narrative Report: 1936: Camp Buena Vista BF-3: 2: August (United States)

    US Fish and Wildlife Service, Department of the Interior — This narrative report from the Civilian Conservation Corps summarizes activities done on Camp Buena Vista. Topics include land development and maintenance, wildlife...

  2. Malheur National Wildlife Refuge: Narrative Report: 1937: Camp Buena Vista BF-3: 5: May (United States)

    US Fish and Wildlife Service, Department of the Interior — This narrative report from the Civilian Conservation Corps summarizes activities done on Camp Buena Vista. Topics include land development and maintenance, wildlife...

  3. Malheur National Wildlife Refuge: Narrative Report: 1936: Camp Buena Vista BF-3: 3: September (United States)

    US Fish and Wildlife Service, Department of the Interior — This narrative report from the Civilian Conservation Corps summarizes activities done on Camp Buena Vista. Topics include land development and maintenance, wildlife...

  4. Fiscal year 1939 : Narrative report : Pea Island Migratory Wildfowl Refuge : Camp BF-2 (United States)

    US Fish and Wildlife Service, Department of the Interior — This 1939 narrative report for Pea Island Migratory Wildfowl Refuge provides a roster of army personnel and service personnel, a summary of camp life, a list of...

  5. Malheur National Wildlife Refuge: Narrative Report: 1936: Camp Buena Vista BF-3: 1: July (United States)

    US Fish and Wildlife Service, Department of the Interior — This narrative report from the Civilian Conservation Corps summarizes activities done on Camp Buena Vista. Topics include land development and maintenance, wildlife...

  6. Malheur National Wildlife Refuge: Narrative Report: 1937: Camp Buena Vista BF-3: 3: March (United States)

    US Fish and Wildlife Service, Department of the Interior — This narrative report from the Civilian Conservation Corps summarizes activities done on Camp Buena Vista. Topics include land development and maintenance, wildlife...

  7. Malheur National Wildlife Refuge: Narrative Report: 1937: Camp Buena Vista BF-3: 4: April (United States)

    US Fish and Wildlife Service, Department of the Interior — This narrative report from the Civilian Conservation Corps summarizes activities done on Camp Buena Vista. Topics include land development and maintenance, wildlife...

  8. Modulation of the cAMP signaling pathway after traumatic brain injury


    Atkins, Coleen M.; Oliva, Anthony A.; Alonso, Ofelia F.; Pearse, Damien D.; Bramlett, Helen M; Dietrich, W. Dalton


    Traumatic brain injury (TBI) results in both focal and diffuse brain pathologies that are exacerbated by the inflammatory response and progress from hours to days after the initial injury. Using a clinically relevant model of TBI, the parasagittal fluid-percussion brain injury (FPI) model, we found injury-induced impairments in the cyclic AMP (cAMP) signaling pathway. Levels of cAMP were depressed in the ipsilateral parietal cortex and hippocampus, as well as activation of its downstream targ...

  9. cAMP signaling in blood platelets - old friends and new players.

    Directory of Open Access Journals (Sweden)

    Zaher eRaslan


    Full Text Available Atherothrombosis, the pathology underlying numerous cardiovascular diseases, is a major cause of death globally. Hyperactive blood platelets play a key role in the atherothrombotic process through the release of inflammatory mediators and formation of thrombi. In healthy blood vessels, excessive platelet activation is restricted by endothelial-derived prostacyclin (PGI2 through cyclic adenosine-5’-monophosphate (cAMP and protein kinase A (PKA-dependent mechanisms. Elevation in intracellular cAMP is associated with the control of a number of distinct platelet functions including actin polymerisation, granule secretion, calcium mobilisation and integrin activation. Unfortunately, in atherosclerotic disease the protective effects of cAMP are compromised, which may contribute to pathological thrombosis. The cAMP signalling network in platelets is highly complex with the presence of multiple isoforms of adenylyl cyclase (AC, PKA and phosphodiesterases (PDE. However, a precise understanding of the relationship between specific AC, PKA and PDE isoforms, and how individual signalling substrates are targeted to control distinct platelet functions is still lacking. In other cells types, compartmentalisation of cAMP signalling has emerged as a key mechanism to allow precise control of specific cell functions. A-kinase anchoring proteins (AKAPs play an important role in this spatiotemporal regulation of cAMP signalling networks. Evidence of AKAP-mediated compartmentalisation of cAMP signalling in blood platelets has begun to emerge and is providing new insights into the regulation of platelet function. Dissecting the mechanisms that allow cAMP to control excessive platelet activity without preventing effective haemostasis may unleash the possibility of therapeutic targeting of the pathway to control unwanted platelet activity.

  10. A study about expectations of the athletes from their coaches resting in Olympics preparation camping Gaziantep


    OZTURK, Huseyin; AVSAR, Omur; CILDIR, Idris


    This is a descriptive study, aims to specify the expectations of the athletes from their coaches resting in Olympic Preparation Camp. A total of 120 athletes resting in Olympic Preparation Camp in Gaziantep participated the research including 94 men and 26 women. The expectation scale (ASABÖ, 2008) was used for collecting the data of this research which was used in scientific study done by Güzel before (7). The scale consist of three sub-dimensions; behavior, coaching model and personality. S...

  11. cAMP prevents TNF-induced apoptosis through inhibiting DISC complex formation in rat hepatocytes. (United States)

    Bhattacharjee, Rajesh; Xiang, Wenpei; Wang, Yinna; Zhang, Xiaoying; Billiar, Timothy R


    Tumor necrosis factor α (TNF) is a pleiotropic proinflammatory cytokine that plays a role in immunity and the control of cell proliferation, cell differentiation, and apoptosis. The pleiotropic nature of TNF is due to the formation of different signaling complexes upon the binding of TNF to its receptor, TNF receptor type 1 (TNFR1). TNF induces apoptosis in various mammalian cells when the cells are co-treated with a transcription inhibitor like actinomycin D (ActD). When TNFR1 is activated, it recruits an adaptor protein, TNF receptor-associated protein with death domain (TRADD), through its cytoplasmic death effector domain (DED). TRADD, in turn, recruits other signaling proteins, including TNF receptor-associated protein 2 (TRAF2) and receptor-associated protein kinase (RIPK) 1, to form a complex. Subsequently, this complex combines with FADD and procaspase-8, converts into a death-inducing signaling complex (DISC) to induce apoptosis. Cyclic AMP (cAMP) is a second messenger that regulates various cellular processes such as cell proliferation, gene expression, and apoptosis. cAMP analogues are reported to act as anti-apoptotic agents in various cell types, including hepatocytes. We found that a cAMP analogue, dibutyryl cAMP (db-cAMP), inhibits TNF+ActD-induced apoptosis in rat hepatocytes. The protein kinase A (PKA) inhibitor KT-5720 reverses this inhibitory effect of cAMP on apoptosis. Cytoprotection by cAMP involves down-regulation of various apoptotic signal regulators like TRADD and FADD and inhibition of caspase-8 and caspase-3 cleavage. We also found that cAMP exerts its affect at the proximal level of TNF signaling by inhibiting the formation of the DISC complex upon the binding of TNF to TNFR1. In conclusion, our study shows that cAMP prevents TNF+ActD-induced apoptosis in rat hepatocytes by inhibiting DISC complex formation.

  12. Clinical Boot Camp: An Innovative Simulation Experience to Prepare Nursing Students for Obstetric and Pediatric Clinicals. (United States)

    Hogewood, Connie; Smith, Tedra; Etheridge, Sherita; Britt, Sylvia


    Obstetric and pediatric patients require unique specialized care not included in traditional adult health education. To prepare nursing students for clinical rotations beginning the second week of class, faculty developed an innovative one-day simulation seminar, the OB/PEDS Boot Camp, in which groups of students rotated through six stations of obstetric and pediatric simulation exercises. This article provides insight on the development and implementation of the OB/PEDS Boot Camp.

  13. Linking cellular actin status with cAMP signaling in Candida albicans. (United States)

    Wang, Yue; Zou, Hao; Fang, Hao-Ming; Zhu, Yong


    The fungal pathogen Candida albicans has a remarkable ability to switch growth forms. Particularly, the yeast-to-hyphae switch is closely linked with its virulence. A range of chemicals and conditions can promote hyphal growth including serum, peptidoglycan, CO2, neutral pH, and elevated temperature. All these signals act essentially through the adenylyl cyclase Cyr1 that synthesizes cAMP. Cells lacking Cyr1 are completely defective in hyphal growth. Recently, cellular actin status is found to influence cAMP synthesis. However, how Cyr1 senses and processes multiple external and internal signals to produce a contextually proper level of cAMP remains unclear. We hypothesized that Cyr1 itself possesses multiple sensors for different signals and achieves signal integration through a combined allosteric effect on the catalytic center. To test this hypothesis, we affinity-purified a Cyr1-containing complex and found that it could enhance cAMP synthesis upon treatment with serum, peptidoglycan or CO2 in vitro. The data indicate that the complex is an essentially intact sensor/effector apparatus for cAMP synthesis. The complex contains two more subunits, the cyclase-associated protein Cap1 and G-actin. We discovered that G-actin plays a regulatory role, rendering cAMP synthesis responsive to actin dynamics. These findings shed new lights on the mechanisms that regulate cAMP-mediated responses in fungi.

  14. Spatiotemporal coupling of cAMP transporter to CFTR chloride channel function in the gut epithelia. (United States)

    Li, Chunying; Krishnamurthy, Partha C; Penmatsa, Himabindu; Marrs, Kevin L; Wang, Xue Qing; Zaccolo, Manuela; Jalink, Kees; Li, Min; Nelson, Deborah J; Schuetz, John D; Naren, Anjaparavanda P


    Cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-regulated chloride channel localized at apical cell membranes and exists in macromolecular complexes with a variety of signaling and transporter molecules. Here, we report that the multidrug resistance protein 4 (MRP4), a cAMP transporter, functionally and physically associates with CFTR. Adenosine-stimulated CFTR-mediated chloride currents are potentiated by MRP4 inhibition, and this potentiation is directly coupled to attenuated cAMP efflux through the apical cAMP transporter. CFTR single-channel recordings and FRET-based intracellular cAMP dynamics suggest that a compartmentalized coupling of cAMP transporter and CFTR occurs via the PDZ scaffolding protein, PDZK1, forming a macromolecular complex at apical surfaces of gut epithelia. Disrupting this complex abrogates the functional coupling of cAMP transporter activity to CFTR function. Mrp4 knockout mice are more prone to CFTR-mediated secretory diarrhea. Our findings have important implications for disorders such as inflammatory bowel disease and secretory diarrhea.

  15. Discovery of a cAMP deaminase that quenches cyclic AMP-dependent regulation. (United States)

    Goble, Alissa M; Feng, Youjun; Raushel, Frank M; Cronan, John E


    An enzyme of unknown function within the amidohydrolase superfamily was discovered to catalyze the hydrolysis of the universal second messenger, cyclic-3',5'-adenosine monophosphate (cAMP). The enzyme, which we have named CadD, is encoded by the human pathogenic bacterium Leptospira interrogans. Although CadD is annotated as an adenosine deaminase, the protein specifically deaminates cAMP to cyclic-3',5'-inosine monophosphate (cIMP) with a kcat/Km of 2.7 ± 0.4 × 10(5) M(-1) s(-1) and has no activity on adenosine, adenine, or 5'-adenosine monophosphate (AMP). This is the first identification of a deaminase specific for cAMP. Expression of CadD in Escherichia coli mimics the loss of adenylate cyclase in that it blocks growth on carbon sources that require the cAMP-CRP transcriptional activator complex for expression of the cognate genes. The cIMP reaction product cannot replace cAMP as the ligand for CRP binding to DNA in vitro and cIMP is a very poor competitor of cAMP activation of CRP for DNA binding. Transcriptional analyses indicate that CadD expression represses expression of several cAMP-CRP dependent genes. CadD adds a new activity to the cAMP metabolic network and may be a useful tool in intracellular study of cAMP-dependent processes.

  16. Forced migration and sexual abuse: experience of Congolese adolescent girls in Kigeme refugee camp, Rwanda

    Directory of Open Access Journals (Sweden)

    Innocent Iyakaremye


    Full Text Available Background This study deals with the link between forced migration and sexual abuse, with a special focus on adolescent girls. Existing literature associates forced migration with sexual abuse and identifies adolescent girls as the most vulnerable. However, little is known about the situation of sexual abuse among Congolese refugees in Rwanda since their arrival in 2012 due to the conflict between Congolese government forces and the M23 rebel group. This study was initiated to explore the situation of sexual abuse of Congolese adolescent girls in Kigeme camp and to suggest remedial strategies. Participants and procedure Qualitative data were collected through individual interviews and focus group discussions (FGDs with adolescent girls. Interviews also involved parents, boys, camp authorities, and neighbouring citizens. Results The findings show that rape, unwanted physical touching, sexual exploitation, commercial sex, early marriage and girl trafficking are the main forms of sexual abuse. These are facilitated by the miserable life in the camp, shortcomings in the camp layout and security system, and adolescent developmental stage. They negatively impact girls’ reproductive health, social integration and mental health. Conclusions Existing strategies to address sexual abuse in the camp have had positive but insufficient results, and thus need to be improved and reinforced. Improvement is suggested in the areas of the abuse reporting system, the camp layout and security system, involvement of men and youth, and the consolidation of anti-GBV (gender-based violence clubs.

  17. The development of a sustainable, community-supported children's bereavement camp. (United States)

    Bachman, Betty


    This article describes the creation and development of a sustainable, community-supported children's bereavement camp. Numerous grief camps were examined prior to the project development. The project development was guided by the S.M.A.R.T. (S--Strategic/specific; M--Measurable; A--Achievable/attainable; R--Realistic; and T--Time-framed) stratagem to direct steps toward the development of the bereavement camp. Outcome measures included program participation, as well as evaluations completed by campers, family members, and volunteers. Camp attendance continues to grow, with 48 children the first year and an average of 65 the following 3 years. According to post-evaluation surveys, campers were able to integrate back into school with a decrease in stress and an increase in their ability to verbalize their grief, share feelings and begin to trust others. One child "got her sparkle back" according to her grandmother. Several campers commented that camp allowed them to see themselves as normal children. The goal of Camp Healing Hearts was that campers would laugh again, and they are.

  18. Epac and PKA: a tale of two intracellular cAMP receptors

    Institute of Scientific and Technical Information of China (English)

    Xiaodong Cheng; Zhenyu Ji; Tamara Tsalkova; Fang Mei


    cAMP-mediated signaling pathways regulate a multitude of important biological processes under both physiological and pathological conditions,including diabetes,heart failure and cancer.In eukaryotic cells,the effects of cAMP are mediated by two ubiquitously expressed intracellular cAMP receptors,the classic protein kinase A (PKA)/cAMP-dependent protein kinase and the recently discovered exchange protein directly activated by cAMP(Epac)/cAMP-regulated guanine nucleotide exchange factors.Like PKA,Epac contains an evolutionally conserved cAMP binding domain that acts as a molecular switch for sensing intracellular second messenger cAMP levels to control diverse biological functions.The existence of two families of cAMP effectors provides a mechanism for a more precise and integrated control of the cAMP signaling pathways in a spatial and temporal manner.Depending upon the specific cellular environments as well as their relative abundance,distrbution and localization,Epac and PKA may act independently,converge synergistically or oppose each other in regulating a specific cellular function.

  19. Regulation of cAMP Intracellular Levels in Human Platelets Stimulated by 2-Arachidonoylglycerol. (United States)

    Signorello, Maria Grazia; Leoncini, Giuliana


    We demonstrated that in human platelets the endocannabinoid 2-arachidonoylglycerol (2-AG) decreased dose- and time-dependently cAMP intracellular levels. No effect on cAMP decrease induced by 2-AG was observed in the presence of the adenylate cyclase inhibitor SQ22536 as well in platelets pretreated with the thromboxane A2 receptor antagonist, SQ29548 or with aspirin, inhibitor of arachidonic acid metabolism through the cyclooxygenase pathway. An almost complete recovering of cAMP level was measured in platelets pretreated with the specific inhibitor of phosphodiesterase (PDE) 3A, milrinone. In platelets pretreated with LY294002 or MK2206, inhibitors of PI3K/AKT pathway, and with U73122, inhibitor of phospholipase C pathway, only a partial prevention was shown. cAMP intracellular level depends on synthesis by adenylate cyclase and hydrolysis by PDEs. In 2-AG-stimulated platelets adenylate cyclase activity seems to be unchanged. In contrast PDEs appear to be involved. In particular PDE3A was specifically activated, as milrinone reversed cAMP reduction by 2-AG. 2-AG enhanced PDE3A activity through its phosphorylation. The PI3K/AKT pathway and PKC participate to this PDE3A phosphorylation/activation mechanism as it was greatly inhibited by platelet pretreatment with LY294002, MK2206, U73122, or the PKC specific inhibitor GF109203X. Taken together these data suggest that 2-AG potentiates its power of platelet agonist reducing cAMP intracellular level.

  20. Spatiotemporal Coupling of cAMP Transporter to CFTR Chloride Channel Function in the Gut Epithelia (United States)

    Li, Chunying; Krishnamurthy, Partha C.; Penmatsa, Himabindu; Marrs, Kevin L.; Wang, Xue Qing; Zaccolo, Manuela; Jalink, Kees; Li, Min; Nelson, Deborah J.; Schuetz, John D.; Naren, Anjaparavanda P.


    SUMMARY Cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-regulated chloride channel localized at apical cell membranes and exists in macromolecular complexes with a variety of signaling and transporter molecules. Here we report that the multidrug resistance protein 4 (MRP4), a cAMP transporter, is functionally and physically associates with CFTR. Adenosine-stimulated CFTR-mediated chloride currents are potentiated by MRP4 inhibition, and this potentiation is directly coupled to attenuated cAMP efflux through the apical cAMP transporter. CFTR single-channel recordings and FRET-based intracellular cAMP dynamics suggest that a compartmentalized coupling of cAMP transporter and CFTR occurs via the PDZ scaffolding protein, PDZK1, forming a macromolecular complex at apical surfaces of gut epithelia. Disrupting this complex abrogates the functional coupling of cAMP transporter activity to CFTR function. MRP4 knockout mice are more prone to CFTR-mediated secretory diarrhea. Our findings have important implications for disorders such as inflammatory bowel disease and secretory diarrhea. PMID:18045536

  1. Can You Hack It? Validating Predictors for IT Boot Camps (United States)

    Gear, Courtney C.

    Given the large number of information technology jobs open and lack of qualified individuals to fill them, coding boot camps have sprung up in response to this skill gap by offering a specialized training program in an accelerated format. This fast growth has created a need to measure these training programs and understand their effectiveness. In the present study, a series of analyses examined whether specific or combinations of predictors were valid for training performance in this coding academy. Self-rated, daily efficacy scores were used as outcome variables of training success and correlation results showed a positive relationship with efficacy scores and the logic test score as a predictor. Exploratory analyses indicated a Dunning-Kruger effect where students with lower education levels experience higher overall mood during the training program. Limitations of the study included small sample size, severe range restriction in predictor scores, lack of variance in predictor scores, and low variability in training program success. These limitations made identifying jumps between training stages difficult to identify. By identifying which predictors matter most for each stage of skill acquisition, further research should consider more objective variables such as instructor scores which can serve as a guideline to better asses what stage learners join at and how to design curriculum and assignments accordingly (Honken, 2013).

  2. Flame Retardant Applications in Camping Tents and Potential Exposure. (United States)

    Keller, Alexander S; Raju, Nikhilesh P; Webster, Thomas F; Stapleton, Heather M


    Concern has mounted over health effects caused by exposure to flame retardant additives used in consumer products. Significant research efforts have focused particularly on exposure to polybrominated diphenyl ethers (PBDEs) used in furniture and electronic applications. However, little attention has focused on applications in textiles, particularly textiles meeting a flammability standard known as CPAI-84. In this study, we investigated flame retardant applications in camping tents that met CPAI-84 standards by analyzing 11 samples of tent fabrics for chemical flame retardant additives. Furthermore, we investigated potential exposure by collecting paired samples of tent wipes and hand wipes from 27 individuals after tent setup. Of the 11 fabric samples analyzed, 10 contained flame retardant additives, which included tris(1,3-dichloroisopropyl) phosphate (TDCPP), decabromodiphenyl ether (BDE-209), triphenyl phosphate, and tetrabromobisphenol-A. Flame retardant concentrations were discovered to be as high as 37.5 mg/g (3.8% by weight) in the tent fabric samples, and TDCPP and BDE-209 were the most frequently detected in these samples. We also observed a significant association between TDCPP levels in tent wipes and those in paired hand wipes, suggesting that human contact with the tent fabric material leads to the transfer of the flame retardant to the skin surface and human exposure. These results suggest that direct contact with flame retardant-treated textiles may be a source of exposure. Future studies will be needed to better characterize exposure, including via inhalation and dermal sorption from air.

  3. Binding of the auxiliary subunit TRIP8b to HCN channels shifts the mode of action of cAMP. (United States)

    Hu, Lei; Santoro, Bina; Saponaro, Andrea; Liu, Haiying; Moroni, Anna; Siegelbaum, Steven


    Hyperpolarization-activated cyclic nucleotide-regulated cation (HCN) channels generate the hyperpolarization-activated cation current Ih present in many neurons. These channels are directly regulated by the binding of cAMP, which both shifts the voltage dependence of HCN channel opening to more positive potentials and increases maximal Ih at extreme negative voltages where voltage gating is complete. Here we report that the HCN channel brain-specific auxiliary subunit TRIP8b produces opposing actions on these two effects of cAMP. In the first action, TRIP8b inhibits the effect of cAMP to shift voltage gating, decreasing both the sensitivity of the channel to cAMP (K1/2) and the efficacy of cAMP (maximal voltage shift); conversely, cAMP binding inhibits these actions of TRIP8b. These mutually antagonistic actions are well described by a cyclic allosteric mechanism in which TRIP8b binding reduces the affinity of the channel for cAMP, with the affinity of the open state for cAMP being reduced to a greater extent than the cAMP affinity of the closed state. In a second apparently independent action, TRIP8b enhances the action of cAMP to increase maximal Ih. This latter effect cannot be explained by the cyclic allosteric model but results from a previously uncharacterized action of TRIP8b to reduce maximal current through the channel in the absence of cAMP. Because the binding of cAMP also antagonizes this second effect of TRIP8b, application of cAMP produces a larger increase in maximal Ih in the presence of TRIP8b than in its absence. These findings may provide a mechanistic explanation for the wide variability in the effects of modulatory transmitters on the voltage gating and maximal amplitude of Ih reported for different neurons in the brain.

  4. TLR-2 Recognizes Propionibacterium acnes CAMP Factor 1 from Highly Inflammatory Strains (United States)

    Ollagnier, Guillaume; Désiré, Nathalie; Sayon, Sophie; Raingeaud, Jöel; Marcelin, Anne-Geneviève; Calvez, Vincent; Khammari, Amir; Batteux, Frédéric; Dréno, Brigitte; Dupin, Nicolas


    Background Propionibacterium acnes (P. acnes) is an anaerobic, Gram-positive bacteria encountered in inflammatory acne lesions, particularly in the pilosebaceous follicle. P. acnes triggers a strong immune response involving keratinocytes, sebocytes and monocytes, the target cells during acne development. Lipoteicoic acid and peptidoglycan induce the inflammatory reaction, but no P. acnes surface protein interacting with Toll-like receptors has been identified. P. acnes surface proteins have been extracted by lithium stripping and shown to induce CXCL8 production by keratinocytes. Methodology and principal findings Far-western blotting identified two surface proteins, of 24.5- and 27.5-kDa in size, specifically recognized by TLR2. These proteins were characterized, by LC-MS/MS, as CAMP factor 1 devoid of its signal peptide sequence, as shown by N-terminal sequencing. Purified CAMP factor 1 induces CXCL8 production by activating the CXCL8 gene promoter, triggering the synthesis of CXCL8 mRNA. Antibodies against TLR2 significantly decreased the CXCL8 response. For the 27 P. acnes strains used in this study, CAMP1-TLR2 binding intensity was modulated and appeared to be strong in type IB and II strains, which produced large amounts of CXCL8, whereas most of the type IA1 and IA2 strains presented little or no CAMP1-TLR2 binding and low levels of CXCL8 production. The nucleotide sequence of CAMP factor displays a major polymorphism, defining two distinct genetic groups corresponding to CAMP factor 1 with 14 amino-acid changes from strains phylotyped II with moderate and high levels of CAMP1-TLR2 binding activity, and CAMP factor 1 containing 0, 1 or 2 amino-acid changes from strains phylotyped IA1, IA2, or IB presenting no, weak or moderate CAMP1-TLR2 binding. Conclusions Our findings indicate that CAMP factor 1 may contribute to P. acnes virulence, by amplifying the inflammation reaction through direct interaction with TLR2. PMID:27902761

  5. Impact of incarceration in Nazi concentration camps on multimorbidity of former prisoners

    Directory of Open Access Journals (Sweden)

    Jablonski RK


    Full Text Available Robert K Jablonski,1 Jerzy Leszek,2 Joanna Rosińczuk,3 Izabella Uchmanowicz,4 Bernard Panaszek11Department and Clinic of Internal Diseases, Geriatry and Allergology, 2Department of Psychiatry, 3Department of Nervous System Diseases, Department of Clinical Nursing, 4Division of Nursing in Internal Medicine Procedures, Department of Clinical Nursing, Wroclaw Medical University, Wroclaw, PolandObjective: To show the extent to which the health of former prisoners was affected by incarceration in extermination camps after 5 and 30 years of leaving the camp, and to determine the etiological factors underlying particular dysfunctions.Methods: Medical records of former prisoners developed in 1950 (n=250 and 1975 (n=120 were then, after several decades, retrospectively analyzed and compared with the control group, randomized and matched according to age, sex, occupation, and environment. None of the subjects in the control group was a prisoner either at a concentration camp or at any other prison or detention facility.Results: Multimorbidity affected mainly the central nervous system (CNS. Five years after leaving a camp, CNS dysfunctions were observed in 66% of former prisoners. Skeletal (42.4% and cardiovascular system (34.4% dysfunctions were the second and third most frequent dysfunctions. Thirty years after leaving a camp, the most prevalent coexisting conditions were also found within the CNS (80%, cardiovascular system (58.33%, and skeletal system (55%. Five and 30 years after leaving a camp, multiorgan lesions were found in 21.6% and 60% of survivors, respectively. Multimorbidity was more frequent in a group of prisoners who underwent the state of apathy and depression or who had been incarcerated longer than 24 months. The rate of CNS diseases was four times higher, and the rate of cardiovascular diseases or skeletal system dysfunctions was two times higher, in the study group after 30 years of leaving a camp compared with the control group

  6. Images d’un camp de vacances en pays socialiste

    Directory of Open Access Journals (Sweden)

    Ania Szczepanska


    Full Text Available En 1976, Marcel Lozinski choisit d’aller filmer un camp de vacances organisé par le mouvement de la jeunesse socialiste dans la région des lacs de Mazurie en Pologne. Le cinéaste décide de filmer le quotidien de ces jeunes familles en vacances, entre quiz politiques, leçons de savoir vivre et concours de la famille modèle. Pour cela, il élabore un protocole de travail singulier : aux vacanciers s’ajoutent des personnes complices du cinéaste dont le rôle sera pour certains de participer activement à la vie collective, pour d’autres de s’y opposer.Tourné en 1976, le documentaire Comment vivre attendra cinq années avant d’être diffusé en salle, en tant que fiction. Pourquoi cette diffusion retardée et surtout, que penser de cette requalification a posteriori ? Outre l’analyse du film lui-même, un entretien mené avec Marcel Lozinski ainsi que des archives consultées à la filmothèque de Varsovie apporteront des éléments d’analyse sur la réception de l’œuvre par les autorités cinématographiques de l’époque, mais également sur le sens produit par les dispositifs mis en place par le cinéaste au cours de ce tournage.

  7. G beta gamma signaling reduces intracellular cAMP to promote meiotic progression in mouse oocytes. (United States)

    Gill, Arvind; Hammes, Stephen R


    In nearly every vertebrate species, elevated intracellular cAMP maintains oocytes in prophase I of meiosis. Prior to ovulation, gonadotropins trigger various intra-ovarian processes, including the breakdown of gap junctions, the activation of EGF receptors, and the secretion of steroids. These events in turn decrease intracellular cAMP levels in select oocytes to allow meiotic progression, or maturation, to resume. Studies suggest that cAMP levels are kept elevated in resting oocytes by constitutive G protein signaling, and that the drop in intracellular cAMP that accompanies maturation may be due in part to attenuation of this inhibitory G protein-mediated signaling. Interestingly, one of these G protein regulators of meiotic arrest is the Galpha(s) protein, which stimulates adenylyl cyclase to raise intracellular cAMP in two important animal models of oocyte development: Xenopus leavis frogs and mice. In addition to G(alpha)(s), constitutive Gbetagamma activity similarly stimulates adenylyl cyclase to raise cAMP and prevent maturation in Xenopus oocytes; however, the role of Gbetagamma in regulating meiosis in mouse oocytes has not been examined. Here we show that Gbetagamma does not contribute to the maintenance of murine oocyte meiotic arrest. In fact, contrary to observations in frog oocytes, Gbetagamma signaling in mouse oocytes reduces cAMP and promotes oocyte maturation, suggesting that Gbetagamma might in fact play a positive role in promoting oocyte maturation. These observations emphasize that, while many general concepts and components of meiotic regulation are conserved from frogs to mice, specific differences exist that may lead to important insights regarding ovarian development in vertebrates.

  8. Preserved cardiac function despite marked impairment of cAMP generation.

    Directory of Open Access Journals (Sweden)

    Mei Hua Gao

    Full Text Available OBJECTIVES: So many clinical trials of positive inotropes have failed, that it is now axiomatic that agents that increase cAMP are deleterious to the failing heart. An alternative strategy is to alter myocardial Ca(2+ handling or myofilament response to Ca(2+ using agents that do not affect cAMP. Although left ventricular (LV function is tightly linked to adenylyl cyclase (AC activity, the beneficial effects of AC may be independent of cAMP and instead stem from effects on Ca(2+ handling. Here we ask whether an AC mutant molecule that reduces LV cAMP production would have favorable effects on LV function through its effects on Ca(2+ handling alone. METHODS AND RESULTS: We generated transgenic mice with cardiac-directed expression of an AC6 mutant (AC6mut. Cardiac myocytes showed impaired cAMP production in response to isoproterenol (74% reduction; p<0.001, but LV size and function were normal. Isolated hearts showed preserved LV function in response to isoproterenol stimulation. AC6mut expression was associated with increased sarcoplasmic reticulum Ca(2+ uptake and the EC50 for SERCA2a activation was reduced. Cardiac myocytes isolated from AC6mut mice showed increased amplitude of Ca(2+ transients in response to isoproterenol (p = 0.0001. AC6mut expression also was associated with increased expression of LV S100A1 (p = 0.03 and reduced expression of phospholamban protein (p = 0.01. CONCLUSION: LV AC mutant expression is associated with normal cardiac function despite impaired cAMP generation. The mechanism appears to be through effects on Ca(2+ handling - effects that occur despite diminished cAMP.

  9. Laurel Clark Earth Camp: Building a Framework for Teacher and Student Understanding of Earth Systems (United States)

    Colodner, D.; Buxner, S.; Schwartz, K.; Orchard, A.; Titcomb, A.; King, B.; Baldridge, A.; Thomas-Hilburn, H.; Crown, D. A.


    Laurel Clark Earth Camp is designed to inspire teachers and students to study their world through field experiences, remote sensing investigations, and hands on exploration, all of which lend context to scientific inquiry. In three different programs (for middle school students, for high school students, and for teachers) participants are challenged to understand Earth processes from the perspectives of both on-the ground inspection and from examination of satellite images, and use those multiple perspectives to determine best practices on both a societal and individual scale. Earth Camp is a field-based program that takes place both in the “natural” and built environment. Middle School Earth Camp introduces students to a variety of environmental science, engineering, technology, and societal approaches to sustainability. High School Earth Camp explores ecology and water resources from southern Arizona to eastern Utah, including a 5 day rafting trip. In both camps, students compare environmental change observed through repeat photography on the ground to changes observed from space. Students are encouraged to utilize their camp experience in considering their future course of study, career objectives, and lifestyle choices. During Earth Camp for Educators, teachers participate in a series of weekend workshops to explore relevant environmental science practices, including water quality testing, biodiversity surveys, water and light audits, and remote sensing. Teachers engage students, both in school and after school, in scientific investigations with this broad based set of tools. Earth Stories from Space is a website that will assist in developing skills and comfort in analyzing change over time and space using remotely sensed images. Through this three-year NASA funded program, participants will appreciate the importance of scale and perspective in understanding Earth systems and become inspired to make choices that protect the environment.

  10. Increases in cAMP, MAPK Activity and CREB Phosphorylation during REM Sleep: Implications for REM Sleep and Memory Consolidation


    Luo, Jie; Phan, Trongha X.; Yang, Yimei; Garelick, Michael G.; Storm, Daniel R.


    The cyclic adenosine monophosphate (cAMP), mitogen-activated protein kinase (MAPK) and cAMP response element-binding protein (CREB) transcriptional pathway is required for consolidation of hippocampus-dependent memory. In mice, this pathway undergoes a circadian oscillation required for memory persistence that reaches a peak during the daytime. Since mice exhibit polyphasic sleep patterns during the day, this suggested the interesting possibility that cAMP, MAPK activity and CREB phosphorylat...

  11. Camp life: Are northern work camps safe havens for a migrant workforce, or dens of iniquity rampant with sex, drugs and alcohol?

    Energy Technology Data Exchange (ETDEWEB)

    Laverty, K.


    Two studies, dealing with life in work camps in northern Alberta and yielding contradictory results, are discussed. One study by a graduate student in sociology found that many of the men and women housed in work camps in remote locations of the northeastern oilsands belt use drugs, alcohol and casual sex to relieve boredom and loneliness. The other study, commissioned by the Athabasca Regional Issues Working Group (RWIG) found that camp workers visit Fort McMurray on the average of just over once a week, and use that time to take care of normal business, such as visiting health care professionals, buying gasoline, clothing, etc. It found no evidence of widespread sex, or drug or alcohol abuse among work camp residents. The RWIG study surveyed 25 per cent of the 6,272 worker population living in three camps in the Wood Buffalo region during June 2003. The study prepared by V. Taylor for a M.A. degree in sociology at the University of Calgary was severely criticized, primarily for its conclusions being based on a sample size of only nine men and one woman. Despite the criticism, the Taylor study made headlines across the country and has been instrumental in raising awareness of the special needs of a mobile workforce. A more broadly-based study is in progress at the University of Alberta, supported by the RCMP and a number of workplace stakeholders. Its objectives are to examine the situation more thoroughly, identify gaps in services and to explore long term solutions to what is undeniably a serious problem, indicated, if not proven, by the Taylor study.

  12. Early Entry for Youth into the Ocean Science Pipeline Through Ocean Science School Camp and Summer Camp Programs: A Key Strategy for Enhancing Diversity in the Ocean Sciences (United States)

    Crane, N. L.; Wasser, A.; Weiss, T.; Sullivan, M.; Jones, A.


    Educators, policymakers, employers and other stakeholders in ocean and other geo-science fields face the continuing challenge of a lack of diversity in these fields. A particular challenge for educators and geo-science professionals promoting ocean sciences is to create programs that have broad access, including access for underrepresented youth. Experiential learning in environments such as intensive multi-day science and summer camps can be a critical captivator and motivator for young people. Our data suggest that youth, especially underrepresented youth, may benefit from exposure to the oceans and ocean science through intensive, sustained (eg more than just an afternoon), hands-on, science-based experiences. Data from the more than 570 youth who have participated in Camp SEA Lab's academically based experiential ocean science camp and summer programs provide compelling evidence for the importance of such programs in motivating young people. We have paid special attention to factors that might play a role in recruiting and retaining these young people in ocean science fields. Over 50% of program attendees were underrepresented youth and on scholarship, which gives us a closer look at the impact of such programs on youth who would otherwise not have the opportunity to participate. Both cognitive (knowledge) and affective (personal growth and motivation) indicators were assessed through surveys and questionnaires. Major themes drawn from the data for knowledge growth and personal growth in Camp SEA Lab youth attendees will be presented. These will be placed into the larger context of critical factors that enhance recruitment and retention in the geo-science pipeline. Successful strategies and challenges for involving families and broadening access to specialized programs such as Camp SEA Lab will also be discussed.

  13. Relaxin Stimulates cAMP Production in MCF-7 Cells upon Overexpression of Type V Adenylyl Cyclase


    Nguyen, Bao T.; Dessauer, Carmen W.


    Relaxin stimulates cAMP production and activation of ERK and PI3K in THP-1 cells. Relaxin also stimulates protein kinase C zeta (PKCζ) translocation to the plasma membrane in a PI3K-dependent manner in THP-1 and MCF-7 cells. However, relaxin did not increase cAMP production in MCF-7 cells. We overexpressed different adenylyl cyclase (AC) isoforms in MCF-7 cells to examine coupling of endogenous relaxin receptors to cAMP production. Overexpression of types II and IV AC had no effect on cAMP pr...

  14. Requirement of cAMP signaling for Schwann cell differentiation restricts the onset of myelination. (United States)

    Bacallao, Ketty; Monje, Paula V


    Isolated Schwann cells (SCs) respond to cAMP elevation by adopting a differentiated post-mitotic state that exhibits high levels of Krox-20, a transcriptional enhancer of myelination, and mature SC markers such as the myelin lipid galactocerebroside (O1). To address how cAMP controls myelination, we performed a series of cell culture experiments which compared the differentiating responses of isolated and axon-related SCs to cAMP analogs and ascorbate, a known inducer of axon ensheathment, basal lamina formation and myelination. In axon-related SCs, cAMP induced the expression of Krox-20 and O1 without a concomitant increase in the expression of myelin basic protein (MBP) and without promoting axon ensheathment, collagen synthesis or basal lamina assembly. When cAMP was provided together with ascorbate, a dramatic enhancement of MBP expression occurred, indicating that cAMP primes SCs to form myelin only under conditions supportive of basal lamina formation. Experiments using a combination of cell permeable cAMP analogs and type-selective adenylyl cyclase (AC) agonists and antagonists revealed that selective transmembrane AC (tmAC) activation with forskolin was not sufficient for full SC differentiation and that the attainment of an O1 positive state also relied on the activity of the soluble AC (sAC), a bicarbonate sensor that is insensitive to forskolin and GPCR activation. Pharmacological and immunological evidence indicated that SCs expressed sAC and that sAC activity was required for morphological differentiation and the expression of myelin markers such as O1 and protein zero. To conclude, our data indicates that cAMP did not directly drive myelination but rather the transition into an O1 positive state, which is perhaps the most critical cAMP-dependent rate limiting step for the onset of myelination. The temporally restricted role of cAMP in inducing differentiation independently of basal lamina formation provides a clear example of the uncoupling of signals

  15. Requirement of cAMP signaling for Schwann cell differentiation restricts the onset of myelination.

    Directory of Open Access Journals (Sweden)

    Ketty Bacallao

    Full Text Available Isolated Schwann cells (SCs respond to cAMP elevation by adopting a differentiated post-mitotic state that exhibits high levels of Krox-20, a transcriptional enhancer of myelination, and mature SC markers such as the myelin lipid galactocerebroside (O1. To address how cAMP controls myelination, we performed a series of cell culture experiments which compared the differentiating responses of isolated and axon-related SCs to cAMP analogs and ascorbate, a known inducer of axon ensheathment, basal lamina formation and myelination. In axon-related SCs, cAMP induced the expression of Krox-20 and O1 without a concomitant increase in the expression of myelin basic protein (MBP and without promoting axon ensheathment, collagen synthesis or basal lamina assembly. When cAMP was provided together with ascorbate, a dramatic enhancement of MBP expression occurred, indicating that cAMP primes SCs to form myelin only under conditions supportive of basal lamina formation. Experiments using a combination of cell permeable cAMP analogs and type-selective adenylyl cyclase (AC agonists and antagonists revealed that selective transmembrane AC (tmAC activation with forskolin was not sufficient for full SC differentiation and that the attainment of an O1 positive state also relied on the activity of the soluble AC (sAC, a bicarbonate sensor that is insensitive to forskolin and GPCR activation. Pharmacological and immunological evidence indicated that SCs expressed sAC and that sAC activity was required for morphological differentiation and the expression of myelin markers such as O1 and protein zero. To conclude, our data indicates that cAMP did not directly drive myelination but rather the transition into an O1 positive state, which is perhaps the most critical cAMP-dependent rate limiting step for the onset of myelination. The temporally restricted role of cAMP in inducing differentiation independently of basal lamina formation provides a clear example of the

  16. Evidence for cAMP as a mediator of gonadotropin secretion from male pituitaries

    Energy Technology Data Exchange (ETDEWEB)

    Bourne, G.A.; Baldwin, D.M.


    The purpose of this study was to use sodium flufenamate, a compound that inhibits gonadotropin-releasing hormone (GnRH)-stimulated adenosine 3',5'-cyclic monophosphate (cAMP) production in the pituitary, to evaluate the potential role of cAMP as a mediator of GnRH-stimulated gonadotropin secretion from male pituitaries. Quartered male pituitaries were perifused at 37/sup 0/C and sequential effluent fractions collected every 10 min. Infusions of GnRH resulted in a twofold increase in luteinizing hormone (LH) and follicle-stimulating hormone (FSH) secretion. Cycloheximide, 5, completely inhibited the GnRH-stimulated LH and FSH secretion. Infusions of 0.1 mM flufenamate had similar effects on gonadotropin secretion as cycloheximide, whereas the administration of 5 mM dibutyryl cAMP in combination with GnRH and flufenamate restored the secretory responses of both hormones. The flufenamate-inhibited GnRH stimulated LH and FSH release, which was restored by DBcAMP and appeared to be protein synthesis dependent and specific for cAMP.These results suggest an indirect role for cAMP as a mediator of gonadotropin secretion from male pituitaries. However, in contrast to female pituitaries, the secretion of these hormones form male pituitaries is completely dependent on cAMP and de novo protein synthesis.

  17. cAMP level modulates scleral collagen remodeling, a critical step in the development of myopia.

    Directory of Open Access Journals (Sweden)

    Yijin Tao

    Full Text Available The development of myopia is associated with decreased ocular scleral collagen synthesis in humans and animal models. Collagen synthesis is, in part, under the influence of cyclic adenosine monophosphate (cAMP. We investigated the associations between cAMP, myopia development in guinea pigs, and collagen synthesis by human scleral fibroblasts (HSFs. Form-deprived myopia (FDM was induced by unilateral masking of guinea pig eyes. Scleral cAMP levels increased selectively in the FDM eyes and returned to normal levels after unmasking and recovery. Unilateral subconjunctival treatment with the adenylyl cyclase (AC activator forskolin resulted in a myopic shift accompanied by reduced collagen mRNA levels, but it did not affect retinal electroretinograms. The AC inhibitor SQ22536 attenuated the progression of FDM. Moreover, forskolin inhibited collagen mRNA levels and collagen secretion by HSFs. The inhibition was reversed by SQ22536. These results demonstrate a critical role of cAMP in control of myopia development. Selective regulation of cAMP to control scleral collagen synthesis may be a novel therapeutic strategy for preventing and treating myopia.

  18. Visitor evaluations of management actions at a highly impacted Appalachian Trail camping area (United States)

    Daniels, M.L.; Marion, J.L.


    Protected area management involves balancing environmental and social objectives. This is particularly difficult at high-use/high-impact recreation sites, because resource protection objectives may require substantial site management or visitor regulation. This study examined visitors? reactions to both of these types of actions at Annapolis Rocks, Maryland, a popular Appalachian Trail camping area. We surveyed visitors before and after implementation of camping policies that included shifting camping to designated newly constructed campsites and prohibiting campfires. Survey results reveal that visitors were more satisfied with all social and environmental indicators after the changes were enacted. An Importance-Performance analysis also determined that management actions improved conditions for factors of greatest concern to campers prior to the changes. Posttreatment visitors were least satisfied with factors related to reduced freedom and to some characteristics of the constructed campsites. Although there was evidence of visitor displacement, the camping changes met management goals by protecting the camping area?s natural resources and improving social conditions.

  19. Eviprostat Activates cAMP Signaling Pathway and Suppresses Bladder Smooth Muscle Cell Proliferation

    Directory of Open Access Journals (Sweden)

    Masayuki Takeda


    Full Text Available Eviprostat is a popular phytotherapeutic agent for the treatment of lower urinary tract symptoms (LUTS. At present, the signaling mechanisms underlying its therapeutic effects are still poorly understood. Given that cAMP has been reported to suppress cell hyperplasia and hypertrophy in various pathological situations, we asked whether the effect of Eviprostat could be ascribed to the activation of the cAMP signaling pathway. In the study, exposure of cAMP response element (CRE-secreted alkaline phosphatase (SEAP (CRE-SEAP-reporter cells to Eviprostat elevated SEAP secretion, which was associated with an increased phosphorylation of vasodilator-stimulated phosphoprotein (VASP and cAMP-response element-binding protein (CREB, as well as enhanced expression of CRE-regulated protein connexin43, indicating an activation of the cAMP signaling pathway. Consistent with these observations, Eviprostat-induced expression of Cx43 was abolished in the presence of adenylyl cyclase inhibitor SQ22536 or PKA inhibitor H89, whereas it was mimicked by adenylyl cyclase activator, forskolin. Further analysis demonstrated that Eviprostat significantly potentiated the effect of phosphodiesterase 3 (PDE3 inhibitor, but not that of PDE4 inhibitor, on CRE activation. Moreover, Eviprostat suppressed PDGF-induced activation of ERK and Akt and inhibited cell proliferation and hillock formation in both mesangial cells and bladder smooth muscle cells. Collectively, activation of the cAMP signaling pathway could be an important mechanism by which Eviprostat exerts its therapeutic effects for LUTS.

  20. Refugees in and out North Africa: a study of the Choucha refugee camp in Tunisia. (United States)

    Dourgnon, Paul; Kassar, Hassène


    In recent years, North African (NA) countries ceased to be emigration-only countries and are now on the verge of becoming immigration as well as transit countries for economic migrants and refugees. Contextual as well as structural long-term factors are driving these changes. The ongoing crises in Africa and the Middle East are prompting strong outflows of refugees, which are likely to induce NA countries to share some common public policy and public health concerns with European countries in a near future. This article highlights some aspects of these changes, from the study of the consequences of the 2011 Libyan crisis in Tunisia. It addresses individual trajectories and health concerns of refugees in and out North Africa from a study of the Choucha camp in Tunisia. The camp opened to immigrants from Libya during the 2011 crisis and accommodated the bulk of the refugees flow to Tunisia until July 2012. The study includes a monographic approach and a qualitative survey in the Choucha camp refugees. We describe the crisis history and the health response with a focus on the camp. We then address refugees' trajectories, and health needs and concerns from the interviews we collected in the camp in April 2012.

  1. The effect of hypoxia on PGE2-stimulated cAMP generation in HMEC-1. (United States)

    Wiktorowska-Owczarek, Anna; Owczarek, Jacek


    Prostaglandin E2 (PGE2) is generated in various cells, including endothelial cells, and is responsible for various functions, such as vascular relaxation and angiogenesis. Effects of PGE2 are mediated via receptors EP1-EP4, among which EP2 and EP4 are coupled to Gs protein which activates adenylate cyclase (AC) and cAMP synthesis. The aim of this work was to study the ability of human microvascular endothelial cells (HMEC-1) to synthesize cAMP in the presence of PGE2, and to determine the effect of hypoxia on the PGE2- stimulated cAMP level. It was decided to evaluate the effect of PGE2 on the secretion of VEGF, an inducer of angiogenesis. In summary, our findings show that PGE2 induces cAMP production, but hypoxia may impair PGE2-stimulated activity of the AC-cAMP signaling pathway. These results suggest that the cardioprotective effect of PGE2/EP4/cAMP may be attenuated during ischemia. Furthermore, this study indicates that the pro-angiogenic effect of PGE2 is not associated with VEGF secretion in HMEC-1 cells.

  2. Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging.

    Directory of Open Access Journals (Sweden)

    Haruki Odaka

    Full Text Available Cyclic AMP is a ubiquitous second messenger, which mediates many cellular responses mainly initiated by activation of cell surface receptors. Various Förster resonance energy transfer-based ratiometric cAMP indicators have been created for monitoring the spatial and temporal dynamics of cAMP at the single-cell level. However, single fluorescent protein-based cAMP indicators have been poorly developed, with improvement required for dynamic range and brightness. Based on our previous yellow fluorescent protein-based cAMP indicator, Flamindo, we developed an improved yellow fluorescent cAMP indicator named Flamindo2. Flamindo2 has a 2-fold expanded dynamic range and 8-fold increased brightness compared with Flamindo by optimization of linker peptides in the vicinity of the chromophore. We found that fluorescence intensity of Flamindo2 was decreased to 25% in response to cAMP. Live-cell cAMP imaging of the cytosol and nucleus in COS7 cells using Flamindo2 and nlsFlamindo2, respectively, showed that forskolin elevated cAMP levels in each compartment with different kinetics. Furthermore, dual-color imaging of cAMP and Ca2+ with Flamindo2 and a red fluorescent Ca2+ indicator, R-GECO, showed that cAMP and Ca2+ elevation were induced by noradrenaline in single HeLa cells. Our study shows that Flamindo2, which is feasible for multi-color imaging with other intracellular signaling molecules, is useful and is an alternative tool for live-cell imaging of intracellular cAMP dynamics.

  3. Genetically-Encoded Yellow Fluorescent cAMP Indicator with an Expanded Dynamic Range for Dual-Color Imaging (United States)

    Odaka, Haruki; Arai, Satoshi; Inoue, Takafumi; Kitaguchi, Tetsuya


    Cyclic AMP is a ubiquitous second messenger, which mediates many cellular responses mainly initiated by activation of cell surface receptors. Various Förster resonance energy transfer-based ratiometric cAMP indicators have been created for monitoring the spatial and temporal dynamics of cAMP at the single-cell level. However, single fluorescent protein-based cAMP indicators have been poorly developed, with improvement required for dynamic range and brightness. Based on our previous yellow fluorescent protein-based cAMP indicator, Flamindo, we developed an improved yellow fluorescent cAMP indicator named Flamindo2. Flamindo2 has a 2-fold expanded dynamic range and 8-fold increased brightness compared with Flamindo by optimization of linker peptides in the vicinity of the chromophore. We found that fluorescence intensity of Flamindo2 was decreased to 25% in response to cAMP. Live-cell cAMP imaging of the cytosol and nucleus in COS7 cells using Flamindo2 and nlsFlamindo2, respectively, showed that forskolin elevated cAMP levels in each compartment with different kinetics. Furthermore, dual-color imaging of cAMP and Ca2+ with Flamindo2 and a red fluorescent Ca2+ indicator, R-GECO, showed that cAMP and Ca2+ elevation were induced by noradrenaline in single HeLa cells. Our study shows that Flamindo2, which is feasible for multi-color imaging with other intracellular signaling molecules, is useful and is an alternative tool for live-cell imaging of intracellular cAMP dynamics. PMID:24959857

  4. 8-OH-DPAT facilitated memory consolidation and increased hippocampal and cortical cAMP production. (United States)

    Manuel-Apolinar, L; Meneses, A


    Animals were submitted to an associative learning task named Pavlovian/instrumental autoshaping (P/I-A) and treated with selective 5-HT1A and 5-HT7 receptor agonists and antagonists. Next, they were sacrificed, their brains removed, dissected and changes on cortical and hippocampal cyclic adenosine monophosphate (cAMP) production were determined. Results revealed that, the 8-OH-DPAT treatment facilitated memory consolidation of autoshaping and that effect was blocked completely by WAY100635 and partially by DR4004. WAY100635 or DR4004 alone had no effect on autoshaping. The cAMP results were complex and yielded no clear relationship to the memory results. Thus, cortical and hippocampal increased on cAMP production was observed following administration of the 5-HT(1A/7) agonist 8-OH-DPAT. The memory effect was, completely or partially, reversed by the selective antagonists WAY100635 (5-HT1A) or DR4004 (5-HT7), respectively.

  5. When "humanitarianism" becomes "development": the politics of international aid in Syria's Palestinian refugee camps. (United States)

    Gabiam, Nell


    In recent years, the United Nations Relief and Works Agency for Palestine Refugees in the Near East (UNRWA) has attempted to go beyond its role as a provider of relief and basic services in Palestinian refugee camps and emphasize its role as a development agency. In this article, I focus on the Neirab Rehabilitation Project, an UNRWA-sponsored development project taking place in the Palestinian refugee camps of Ein el Tal and Neirab in northern Syria. I argue that UNRWA's role as a relief-centered humanitarian organization highlights the everyday suffering of Palestinian refugees, suffering that has become embedded in refugees’ political claims. I show that UNRWA's emphasis on “development” in the refugee camps is forcing Palestinian refugees in Ein el Tal and Neirab to reassess the political narrative through which they have understood their relationship with UNRWA.

  6. Hydromania II: Journey of the Oncorhynchus. Summer Science Camp Curriculum 1994.

    Energy Technology Data Exchange (ETDEWEB)

    Moura, Joan; Swerin, Rod


    The Hydromania II curriculum was written for the third in a series of summer science camp experiences targeting students in grades 4--6 who generally have difficulty accessing supplementary academic programs. The summer science camp in Portland is a collaborative effort between Bonneville Power Administration (BPA), the US Department of Energy (DOE), and the Portland Parks and Recreation Community Schools Program along with various other cooperating businesses and organizations. The curriculum has also been incorporated into other summer programs and has been used by teachers to supplement classroom activities. Camps are designed to make available, affordable learning experiences that are fun and motivating to students for the study of science and math. Inner-city, under-represented minorities, rural, and low-income families are particularly encouraged to enroll their children in the program.

  7. Benefits of a mainstreamed summer camp experience for teens with ESRD. (United States)

    Klee, K M


    This is a report on a pilot project integrating children with end stage renal disease (ESRD) with well children for a summer camp experience. As the teen with ESRD prepares to enter the work force and college, he/she will have to adapt to a variety of situations that will not adapt to his/her unique medical condition. These issues motivated a pilot project in which 9 ESRD children were mainstreamed into a 2 week, YMCA summer camp experience. Pre and post questionnaires were developed and distributed to the camper, family, and the cabin counselor along with interviews to assess the value of the experience. All the children left camp more independent and knowledgeable about their self-care. The results of this pilot project indicate that children with ESRD can adapt to their environment and increase independence, self-care and self-esteem through supervised mainstreamed experiences.

  8. Different effects of transcriptional regulators MarA, SoxS and Rob on susceptibility of Escherichia coli to cationic antimicrobial peptides (CAMPs): Rob-dependent CAMP induction of the marRAB operon. (United States)

    Warner, Douglas M; Levy, Stuart B


    Cationic antimicrobial peptides (CAMPs), a component of the mammalian immune system, protect the host from bacterial infections. The roles of the Escherichia coli transcriptional regulators MarA, SoxS and Rob in susceptibility to these peptides were examined. Overexpression of marA, either in an antibiotic-resistant marR mutant or from a plasmid, decreased bacterial susceptibility to CAMPs. Overexpression of the soxS gene from a plasmid, which decreased susceptibility to antibiotics, unexpectedly caused no decrease in CAMP susceptibility; instead it produced increased susceptibility to different CAMPs. Deletion or overexpression of rob had little effect on CAMP susceptibility. The marRAB operon was upregulated when E. coli was incubated in sublethal amounts of CAMPs polymyxin B, LL-37 or human beta-defensin-1; however, this upregulation required Rob. Deletion of acrAB increased bacterial susceptibility to polymyxin B, LL-37 and human beta-defensin-1 peptides. Deletion of tolC yielded an even greater increase in susceptibility to these peptides and also led to increased susceptibility to human alpha-defensin-2. Inhibition of cellular proton-motive force increased peptide susceptibility for wild-type and acrAB deletion strains; however, it decreased susceptibility of tolC mutants. These findings demonstrate that CAMPs are both inducers of marA-mediated drug resistance through interaction with Rob and also substrates for efflux in E. coli. The three related transcriptional regulators show different effects on bacterial cell susceptibility to CAMPs.

  9. Professional burnout of personnel working in summer camps of people with disabilities

    Directory of Open Access Journals (Sweden)

    Giannia N.


    Full Text Available Introduction: Professional burnout constitutes a syndrome of physical and mental exhaustion occurring in working environment with symptoms that are present after frequent and long-term exposure in difficult working conditions especially in special education settings where a higher burnout in noticed due to tne nature of the profession. Purpose: The purpose of this study was to examine professional burnout of working personnel in summer camps for people with disabilities. Materials – Methods: The sample consisted of 35 participants (11 men and 25 women all camp personnel escorting people with disabilities in summer camps. Each participant completed the Maslach Burnout Inventory (Maslach & Jackson, 1986 comprised of 22 items that assess the variables of exhaustion, accomplishment and depersonalization. Omega total reliability analysis revealed an internal consistency ranging from good (ω total= .72 to high (ω total = .91 and a positive interaction between all factors. Independent samples t-test revealed differences personal accomplishment (t = -1.9 , df = 33, p = 0.32 και depersonalization t = -2.59 , df = 33, p = .014 according to participation in physical activity during the summer camp period. ANOVA analysis showed statistically significant differences in depersonalization (F (3, 33 = 2.81, p = .043, η2 =.076 , exhaustion (F (3, 33 = .335, p = .032, η2 =.084 , and personal accomplishment (F (3, 33 = 4.67, p = .008, η2 =.090 in relation to work satisfaction and type of disability. Conclusions: Professional burnout of personnel working in summer camps for people with disabilities appears high due to many hours and high working load that is affected by the nature of disability, participation of personnel in physical activities and their satisfaction derived from primary profession other than summer camp work.

  10. Geophysical Summer Field Camp: Answering questions about the subsurface for the local community (United States)

    van Wijk, K.; Batzle, M.; Liberty, L.; Raynolds, R.


    Summer Geophysics Field Camp is part of the core requirement for undergraduate Geophysics majors at Boise State University (CSM), as well as at Colorado School of Mines (CSM). We have found it to be most effectively taught when the target of the camp involves answering questions, which impact society. For example, currently the CSM/BSU geophysics summer camp focuses on ground water resources and geothermal potential in the Upper Arkansas River Basin, a part of the Rio Grande Rift system in Chaffee County, Colorado. A prime goal is to train students how to combine diverse sources of information into a unified interpretation: Students examine lithologies and structures on the periphery of the basin. Cross sections are constructed to predict the geophysical signature. Geophysical tools then are used to ascertain the gross structure and examine subsurface conditions in greater detail. These tools include surveying, regional gravity, deep and shallow seismic surveys, magnetics, DC resistivity, Ground Penetrating Radar, electromagnetics, hydrochemistry, and karaoke. While BSU and CSM own a considerable amount of geophysical hardware, our field camps are only possible because of extensive support by corporations and governmental agencies. In addition, the Society of Exploration Geohysics (SEG) Foundation provides financial support, Chaffee County assists with housing costs, and local land owners provide open access. In turn, the field camp results aid the community of Chaffee County in assessing their water resources for long term growth planning, as well as understanding the geothermal potential for hydroelectric power generation. BSU is currently exploring with the SEG Foundation under the Geophysicists Without Borders program to apply this model of combined education and social outreach in the form a geophysics camp for Southeast Asia, where we propose to study geohazards,geoarcheology and groundwater issues.

  11. Evidences for involvement of endogenous cAMP in Arabidopsis defense responses to Verticillium toxins

    Institute of Scientific and Technical Information of China (English)

    Jing JIANG; Ling Wen FAN; Wei Hua WU


    Although there were reports suggesting the involvement of endogenous cAMP in plant defense signaling cascades,there is no direct evidence supporting this notion yet and the detailed mechanism is unclear. In the present study, we have used pathogenic fungi Verticillium dahliae and Arabidopsis plants as a model system of plant-microb interaction to demonstrate the function of endogenous cAMP in Arabidopsis defense responses. Both V. dahliae inoculation and Verticillium toxins injection induced typical "wilt" symptoms in Arabidopsis seedlings. When either 8-Br-AMP (a membrane permeable cAMP analogue) or salicylic acid (SA) was applied to Arabidopsis, the plants became resistant to V. dahliae toxins. However, addition of 8-Br-AMP did not increase the resistance of Arabidopsis transgenic plants deficient in SA to the toxins, suggesting that cAMP might act upstream of SA in plant defense signaling pathway.Indeed, 8-Br-cAMP and forskolin, an activator of adenylyl cyclase, significantly stimulated the endogenous SA level in plants, whereas DDA, an inhibitor of adenylyl cyclase dramatically reduced toxin-induced SA increase. Both the endogenous cAMP and SA increased significantly in Arabidopsis seedlings treated with toxins. Furthermore, transcription level of pathogenesis-related protein 1 gene (PR1) was strongly induced by both 8-Br-cAMP and the toxin treatment. Taken together, our data demonstrate that endogenous cAMP is involved in plant defense responses against Verticilliumsecreted toxins by regulating the production of the known signal SA in plant defense pathway.

  12. Cooperation between cAMP signalling and sulfonylurea in insulin secretion. (United States)

    Shibasaki, T; Takahashi, T; Takahashi, H; Seino, S


    Although glucose is physiologically the most important regulator of insulin secretion, glucose-induced insulin secretion is modulated by hormonal and neural inputs to pancreatic β-cells. Most of the hormones and neurotransmitters evoke intracellular signals such as cAMP, Ca²⁺ , and phospholipid-derived molecules by activating G protein-coupled receptors (GPCRs). In particular, cAMP is a key second messenger that amplifies insulin secretion in a glucose concentration-dependent manner. The action of cAMP on insulin secretion is mediated by both protein kinase A (PKA)-dependent and Epac2A-dependent mechanisms. Many of the proteins expressed in β-cells are phosphorylated by PKA in vitro, but only a few proteins in which PKA phosphorylation directly affects insulin secretion have been identified. On the other hand, Epac2A activates the Ras-like small G protein Rap in a cAMP-dependent manner. Epac2A is also directly activated by various sulfonylureas, except for gliclazide. 8-pCPT-2'-O-Me-cAMP, an Epac-selective cAMP analogue, and glibenclamide, a sulfonylurea, synergistically activate Epac2A and Rap1, whereas adrenaline, which suppresses cAMP production in pancreatic β-cells, blocks activation of Epac2A and Rap1 by glibenclamide. Thus, cAMP signalling and sulfonylurea cooperatively activate Epac2A and Rap1. This interaction could account, at least in part, for the synergistic effects of incretin-related drugs and sulfonylureas in insulin secretion. Accordingly, clarification of the mechanism of Epac2A activation may provide therapeutic strategies to improve insulin secretion in diabetes.

  13. Discrimination Study with the Man-Portable Vector (MPV) EMI Sensor at Former Camp Beale, California (United States)


    accuracy relative to  RTK   GPS : IVS located in open field Daily survey 10 MPV Demonstration at Camp Beale ‐ SKY RESEARCH 11 MPV Demonstration at Camp Beale...positional accuracy with GPS was severely degraded. Data collected in the field, on the Instrument Verification Strip (IVS) and test pit were made...accuracy with GPS was severely degraded. Data collected in the field, on the Instrument Verification Strip (IVS) and test pit were made available for

  14. Biophysical Techniques for Detection of cAMP and cGMP in Living Cells

    Directory of Open Access Journals (Sweden)

    Viacheslav O. Nikolaev


    Full Text Available Cyclic nucleotides cAMP and cGMP are ubiquitous second messengers which regulate myriads of functions in virtually all eukaryotic cells. Their intracellular effects are often mediated via discrete subcellular signaling microdomains. In this review, we will discuss state-of-the-art techniques to measure cAMP and cGMP in biological samples with a particular focus on live cell imaging approaches, which allow their detection with high temporal and spatial resolution in living cells and tissues. Finally, we will describe how these techniques can be applied to the analysis of second messenger dynamics in subcellular signaling microdomains.

  15. Communication and Shared Practices are Bringing NASA STEM Resources to Camp Youth (United States)

    LaConte, K.; Shaner, A.; Shipp, S.; Garst, B.; Bialeschki, M. D.; Netting, R.; Erickson, K.


    In 2012, NASA and the American Camp Association (ACA) entered into an alliance to further both organizations' goals and objectives with regard to science, technology, engineering, and mathematics (STEM) education. This alliance is providing camp staff—and their young audiences—access to NASA's resources. NASA disseminates resources (e.g., pathways for requesting guest presenters, informal learning lesson plans), conducts ACA professional development (online and at ACA conferences), and coordinates efforts around key events (e.g., spacecraft launches). ACA promotes awareness of NASA resources through their communications and services. Together, the organizations are working to inspire a new generation of scientists, engineers, explorers, educators, and innovators to pursue STEM careers.

  16. Growth hormone-releasing hormone stimulates cAMP release in superfused rat pituitary cells.


    Horváth, J E; Groot, K. de; Schally, A V


    The release of growth hormone (GH) and cAMP was studied in superfused rat pituitary cells by infusing growth hormone-releasing hormone (GHRH) at different doses or a combination of GHRH and somatostatin 14 (SS-14). Three-minute pulses of GHRH caused a dose-dependent GH and cAMP release (effective concentration of 50% of the maximal biological effect is 0.21 nM and 52.5 nM, respectively). The lowest effective doses of GHRH in the superfusion system were 0.03 nM for GH release and 0.3 nM for cA...

  17. Rock falls from Glacier Point above Camp Curry, Yosemite National Park, California (United States)

    Wieczorek, Gerald F.; Snyder, James B.


    A series of rock falls from the north face of Glacier Point above Camp Curry, Yosemite National Park, California, have caused reexamination of the rock-fall hazard because beginning in June, 1999 a system of cracks propagated through a nearby rock mass outlining a future potential rock fall. If the estimated volume of the potential rock fall fails as a single piece, there could be a risk from rock-fall impact and airborne rock debris to cabins in Camp Curry. The role of joint plane orientation and groundwater pressure in the fractured rock mass are discussed in light of the pattern of developing cracks and potential modes of failure.

  18. Nobody Can See Atoms: Science Camps Highlighting Approaches for Making Chemistry Accessible to Blind and Visually Impaired Students (United States)

    Wedler, Henry B.; Boyes, Lee; Davis, Rebecca L.; Flynn, Dan; Franz, Annaliese; Hamann, Christian S.; Harrison, Jason G.; Lodewyk, Michael W.; Milinkevich, Kristin A.; Shaw, Jared T.; Tantillo, Dean J.; Wang, Selina C.


    Curricula for three chemistry camp experiences for blind and visually impaired (BVI) individuals that incorporated single- and multiday activities and experiments accessible to BVI students are described. Feedback on the camps from students, mentors, and instructors indicates that these events allowed BVI students, who in many cases have been…

  19. Developing water and sanitation services in refugee settings from emergency to sustainability - The case of Zaatari Camp in Jordan

    NARCIS (Netherlands)

    Van der Helm, A.W.C.; Bhai, A.; Coloni, F.; Koning, W.J.G.; De Bakker, P.T.


    Three years after Zaatari camp was established in the Hashemite Kingdom of Jordan, to host Syrian refugees, its population has grown to 82,000 persons. Zaatari is one of the largest refugee camps in the world, in one of the most water scarce areas on earth. Since its establishment, drinking water ha

  20. High incidence of subcutaneous emphysema in children in a Somali refugee cAMP during measles outbreak. (United States)

    Moons, Peter; Thallinger, Monica


    During an outbreak of measles in a refugee camp in Ethiopia, 9 patients (age range 4 months to 18 years) were diagnosed with subcutaneous emphysema. Incidence of this rare complication of measles in this refugee camp was higher than previously reported. We hypothesize that the high incidence is most likely related to poor physical state of the refugee population with high rates of malnutrition.

  1. 78 FR 46598 - Notice of Availability of the Draft Rob Jaggers Camping Area Business Plan and Expanded Amenity... (United States)


    ... Bureau of Land Management Notice of Availability of the Draft Rob Jaggers Camping Area Business Plan and... prepared and is making available to the public the Draft Rob Jaggers Camping Area Business Plan and... written comments on the Draft Business Plan by December 15, 2013. ADDRESSES: Copies of the Draft...

  2. Camp Creates a World of Magic: The Trail to Innovative Thinking Begins at the ACA National Conference. (United States)

    Coleman, Marla


    The American Camping Association 2001 National Conference at Walt Disney World draws parallels between the administration of camp programs and practices at Disney World. Seminars led by Disney managers focus on recruitment of college students, development of a corporate culture and philosophy, emphasis on environment, and quality service that…

  3. Development and evaluation of a learner-centered educational summer camp program on soft skills for baccalaureate nursing students. (United States)

    Lau, Ying; Wang, Wenru


    The objectives were to develop a learner-centered educational camp program for nursing students and to evaluate 4 areas of soft skills, communication ability, clinical interaction, interpersonal relationships, and social problem solving, before and after the program. The results showed that the summer camp program was effective in improving nursing students' soft skills.

  4. Change Agent Research on the BANA-Can/Am Summer Camp for Young People with Eating Disorders. (United States)

    Moriarty, Dick; And Others

    This document reports on the model and method used to design, implement, coordinate, and evaluate a summer camp for young people with eating disorders. The basic approach used at the camp is described as the Sports Institute for Research model, a systems analysis model which focuses on: (1) the ultimate goal or mission; (2) obstacles or problems…

  5. Burn-injured adolescents report gaining multiple developmental benefits and improved life skills as a result of burn camp attendance. (United States)

    Rimmer, Ruth Brubaker; Pressman, Melissa S; Takach, Oliver P; Bay, R Curtis; Croteau, Renee; Hansen, Linda D; Foster, Kevin N; Caruso, Daniel M


    Anecdotally, burn camp has been reported to be a positive developmental and rehabilitative experience for attendees; there is little empirical data to support this belief. This study sought to explore whether burn camp either directly or indirectly elicits positive development outcomes in pediatric burn survivors or increases their psychosocial well-being and achievement. The Youth Experience Survey 2.0, a 66-item self-report inventory designed to measure developmental experiences in an organized youth activity, was administered to children aged 11 to 18 years attending summer burn camp. One hundred and ten burn-injured youth, 58 male and 52 female, reported that burn camp had positively impacted their lives through improved identity exploration, goal-setting and problem-solving abilities, increased physical activity, communication, emotional regulation, and time management skills (P camp for more than 5 years resulted in greater improvement. Study results support the burn camp experience as a far-reaching and positive developmental activity. Participants credited the camp experience with helping them with identity formation and reflection, improved social interactions, and increased initiative; all positive developmental outcomes for youth. Results suggest that burn camp participation not only helps burn-injured youth to deal with their burns but also assists them in the development of social and basic life skills, which will allow them to navigate the transition from youth to adulthood, more effectively and successfully.

  6. Relationship between Adaptation of the Folic Acid and the cAMP Mediated cGMP Response in Dictyostelium

    NARCIS (Netherlands)

    Haastert, Peter J.M. van


    Chemotactic stimulation of post-vegetative Dictyostelium cells with folic acid or aggregative cells with cAMP results in a fast transient cGMP response which peaks at 10 s; basal levels are recovered in about 30-40 s. Stimulation with folic acid or cAMP rapidly desensitizes the cells for equal or lo

  7. Notes from the field: mortality among refugees fleeing Somalia--Dadaab refugee camps, Kenya, July-August 2011. (United States)


    Refugee camps in Dadaab, Kenya, currently are receiving Somali refugees fleeing famine and armed conflict at a rate of approximately 1,400 refugees per day. New arrivals are at an elevated risk for mortality because of severe famine in Somalia, the dangerous journey, and overcrowding in the camps.

  8. Writing and Teaching behind Barbed Wire: An Exiled Composition Class in a Japanese-American Internment Camp (United States)

    Wu, Hui


    By reflecting on Japanese internment camps executed by the U.S. government in World War II, this article examines camp schools' curricula and writing assignments and an English teacher's response to student essays to show how racially profiled students and their Caucasian teacher negotiated the political meanings of civil rights and freedom.…

  9. Camp of Hip-Hop - kõigile kohustuslik / Mari Hiiemäe ; kommenteerinud Joel Juht

    Index Scriptorium Estoniae

    Hiiemäe, Mari


    Üheksandat korda toimuvast rahvusvahelisest tantsulaagrist ja tänavakultuuri tutvustavast noortelaagrist Camp of Hip-Hop, mis toimub Lääne Virumaal Käsmus. 28. juunil toimub kõigile huvilistele meelelahutusüritus Camp of Hip-Hop Championships, kus näitavad oma tantsuoskusi laagris osalejad ja maailmas tunnustatud koreograafid

  10. Effects of Short Term Camp Periods on Aerobic and Anaerobic Performance Parameters in Ice Hockey National Team Athletes (United States)

    Eler, Serdar


    This study was conducted for determining the effects of trainings, applied to athletes during short term camp period, on their aerobic and anaerobic performance. Measurements were made by the participation of 28 volunteer male ice hockey national team players. During the 15-day camp period, 10-minute running and stretching for warming and then…

  11. Cyclic AMP (cAMP) confers drug resistance against DNA damaging agents via PKAIA in CML cells. (United States)

    Xiao, Ling-Yi; Kan, Wai-Ming


    Cyclic adenosine monophosphate (cAMP) regulates many vital functions such as metabolism, proliferation, differentiation and death. Depending on cell types and stimulators, cAMP could either promote or attenuate cell death. cAMP signal can be transduced by protein kinase A (PKA) and/or exchange protein directly activated by cAMP (EPAC). In CML cells, cAMP may suppress their proliferation and enhance their differentiation. However, the role of cAMP on DNA damaging agent toxicity and the mechanism involved has not been studied. In this study, we studied the effect of cAMP on the sensitivity of CML cells to DNA damaging agents. We observed that forskolin (FSK) and dibutyryl-cAMP (DBcAMP) decreased cisplatin and etoposide-induced cell death in K562 cells. Moreover, PKA activator prevented K562 cells from DNA damaging agent-induced cell death while EPAC activator had no effect. Furthermore, we found that the PKA subtype, PKAIA, was involved in cAMP-attenuated resistance in K562 cells. Taken together, our results suggest that increased cAMP level confers CML cells to acquire a novel mechanism against DNA damaging agent toxicity via PKAIA. Thus, PKAIA inhibitor may be helpful in overcoming the resistance to DNA damaging agents in CML cells.

  12. Current Practices for Training Staff to Accommodate Youth with Special Health Care Needs in the 4-H Camp Setting (United States)

    Mouton, Lauren; Bruce, Jacklyn


    The theory of inclusion is the foundation for the study reported here; inclusion is a focus not only of formal education, but also of nonformal educational settings such as 4-H. Ideally, 4-H camps are designed to serve youth of all backgrounds and abilities. By accommodating youth with special health care needs, 4-H camps are effectively meeting…

  13. An evaluation of productivity and mortality factors influencing goose populations: A status report of the 1984 waterfowl monitoring effort at Old Chevak field camp (United States)

    US Fish and Wildlife Service, Department of the Interior — During spring and summer 1984 data were obtained from nine field camps located on the Yukon Delta National Wildlife Refuge one cooperative camp and eight refuge...

  14. Determination of Trace Level of cAMP in Locusta Migratoria Manilensis Meyen by HPLC with Fluorescence Derivation

    Directory of Open Access Journals (Sweden)

    Canping Pan


    Full Text Available A sensitive and rapid method was developed for the determination of cAMP inLocusta migratoria manilensis Meyen by high-performance liquid chromatography withfluorescence detection. The cAMP was derivatized using chloroacetaldehyde and TBASbuffer/methanol was used as the mobile phase. A detection quantification of 40 fmol/mlcould be achieved when using fluorescence detection. An HPLC-MS method using DMHAas an ion-pair agent to analyze cAMP was also demonstrated. We studied the effect ofdopamine and other stimulants on cAMP levels from isolated locust central nervoussystems. The new method is well suited for the analysis of cAMP in small biologicalsamples.

  15. Baccalaureate nursing student experiences at a camp for children with diabetes: the impact of a service-learning model. (United States)

    Vogt, Marjorie A; Chavez, Ruth; Schaffner, Barbara


    Camp nurses deliver health care in a non-traditional setting. Research indicates that camp has a positive impact on campers with diabetes mellitus, but the impact on student nurse knowledge of diabetes care and confidence in the role as a member of the health care team is unknown. This study examined the experiences of student nurses at a summer residential camp for children with diabetes using a service-learning framework. Camp positively increased knowledge of diabetes in the nursing students. Reflective journals identified themes of anxiety, fatigue, responsibility, and increased confidence in the nursing student, while developing empathy for the lifestyle of children with diabetes. Future research on camp nursing is indicated.

  16. Geochemistry of eastern North American CAMP diabase dykes (United States)

    Callegaro, S.; Marzoli, A.; Bertrand, H.; Reisberg, L. C.; Chiaradia, M.; Bellieni, G.


    The Piedmont area of the Appalachians and the coastal plains of eastern North America (ENA) were intruded between 202 and 195 Ma[1,2] by swarms of diabase (dolerite) dykes and a few sills of the Central Atlantic magmatic province (CAMP). Different trends are observed for the dykes, from NW (more frequent in the South), to N-S and NE, thus a radial pattern and a coeval emplacement driven by the bulging effect of a mantle plume were initially inferred[3]. Conversely, based on field data, an age progression can be defined from NW- to N- and NE-oriented dykes, supporting a diachronous northward rift-to-drift transition during Pangaea break-up. New geochemical data on 74 ENA dykes suggest a dominant lithospheric mantle source for these magmatic suite. A deep enriched mantle source is further discarded by the crystallization temperatures (ca.1350°C) calculated[4] for high-Fo (up to Fo89) olivines, which do not reflect very hot (i.e. mantle-plume) potential mantle temperatures. Incompatible trace element contents are fairly homogeneous and generally low, e.g. (La/Yb)Ch (0.54-2.39), typical of melts derived from a quite depleted shallow (spinel) mantle-source. However, isotopic compositions of ENA dykes display a considerable spread in initial isotopic signatures, do not show correlation with incompatible trace elements, and are independent of the orientation and age of the dykes, i.e. 87Sr/86Sr200Ma (0.7043-0.7088), ɛNd200Ma (-6.67-+2.42) and 206Pb/204Pb200Ma (17.46-18.61). Pb isotopic compositions plot above the NHRL, at positive Δ7/4 (10-17) and Δ8/4 (19-73), calling for an enriched ancient component in the mantle source. Unradiogenic 187Os/188Os200Ma ratios (0.127-0.144) argue for negligible amounts of crustal contamination and, coupled with the large range of Sr-Nd-Pb isotopic compositions, suggest generation from a strongly heterogeneous mantle source, probably metasomatized lithosphere. Lithospheric mantle underlying the Appalachian orogen may have undergone

  17. Environmental effects of dredging. Preliminary guidelines and conceptual framework for comprehensive analysis of migration pathways (CAMP) of contaminated dredged material. Technical notes

    Energy Technology Data Exchange (ETDEWEB)

    Myers, T.E.


    The purpose of this note is to present the conceptual groundwork for the Comprehensive Analysis of Migration Pathways (CAMP). The conceptualization process for CAMP is discussed and available techniques for implementing CAMP are examined. Disposal of contaminated dredged material in a confined disposal facility is used to benchmark conceptual development. Case studies that illustrate analysis of selected migration pathways are also described.

  18. Impact of Conflict in Syria on Syrian Children at the Zaatari Refugee Camp in Jordan (United States)

    Jabbar, Sinaria Abdel; Zaza, Haidar Ibrahim


    This paper describes a study performed to investigate the impact of the conflict in Syria on Syrian refugee children. The Zaatari refugee camp in Jordan was chosen for this task. Two control (comparison) groups of children were selected: one from the Jordanian Ramtha district, which is just across the border from Syria, and that indirectly feel…

  19. Evidence for cAMP as a mediator of gonadotropin secretion from female pituitaries

    Energy Technology Data Exchange (ETDEWEB)

    Bourne, G.A.; Baldwin, D.M.


    Sodium flufenamate, which inhibited gonadotropin-releasing hormone (GnRH)-stimulated increases in adenosine 3',5'-cyclic monophosphate (cAMP), was used to evaluate the potential role of cAMP as a mediator of GnRH-stimulated gonadotropin secretion. Quartered pituitaries from diestrous II female rats were perifused at 37/sup 0/C, and sequential effluent fractions were collected every 10 min. Administration of GnRH resulted in a characteristic biphasic response for both luteinizing hormone (LH) and follicle-stimulating hormone (FSH), whereas 5 cycloheximide inhibited the secondary augmented responses (phase II) of both hormones. Infusions of 0.1 mM flufenamate inhibited GnRH-stimulated gonadotropin secretion in a manner similar to that of cycloheximide, whereas the administration of 5 mM dibutyryl cAMP in combination with GnRH and flufenamate resulted in the restoration of LH and FSH secretion. The dibutyryl cAMP-restored response appeared to be protein synthesis dependent and specific for cAMP. These results suggest that although the cyclic nucleotide is not involved in the acute release of LH and FSH, it does appear to play a pivotal but indirect role in phase II release of the hormones, by effects involving the stimulation of de novo protein synthesis.

  20. Sustainability Logistics Basing Science and Technology Objective. Demonstration #1 - 1000 Person Camp Demo (United States)


    69  Table 16: Energy , Fuel and Water Data for Meal sustainment/logistics elements (e.g., energy , fuel , water, waste, etc.). It can provide Contingency Base commanders with information that could...assist in making informed decisions about the management of energy , fuel , water, and waste.  Hybrid Power Trailer (HPT) – electrical power generation

  1. Balancing Educational Practice with Psychological Theory: Lukinsky's Study of a Bold Camp Ramah Curriculum (United States)

    Reimer, Joseph


    Missing from the growing literature on Jewish camps is Lukinsky's (1968) pioneering study of the curriculum to teach responsibility that he designed for the 1966 Ramah American Seminar. Reviewing this work I discovered that Lukinsky--under Schwab's (1971) influence--creates a rare balance between his own perspectives as an educational practitioner…

  2. Outdoor Centers and Camps: A 'Natural' Location for Youth Leadership Development. ERIC Digest. (United States)

    Kielsmeier, James C.

    This digest offers camp leaders, outdoor experiential educators, school and college faculty, or youth agency staff who are interested in nurturing youth leaders a framework for designing youth leadership programs which employ an outdoor setting as a "leadership classroom." Basic categories for leadership theory are defined: trait theory,…

  3. An innovative summer camp program improves weight and self-esteem in obese children (United States)

    To determine the potential benefits of a residential summer camp to treat childhood obesity, 21 obese, multiethnic children (aged 11.4 +/- 1.4 years; body mass index [BMI] percentile 98.5 +/- 1.4; BMI z score 2.30 +/- 0.33) from a diverse socioeconomic background were enrolled in a 2-week summer cam...

  4. Summer camp and self-esteem of school-age inner-city children. (United States)

    Readdick, Christine A; Schaller, G Robert


    The present study was designed to test the hypothesis that a session of summer camp would increase the self-esteem of economically disadvantaged, school-age children from New York's inner-city neighborhoods. This study was conducted at a small, coeducational residential summer camp in the Pocono Mountains designed for children ages 6-12 years from low-income areas of New York City. During each of four 12-day sessions, the Piers-Harris Children's Self-concept Scale was administered as a pretest and posttest to a sample of 68 children (36 boys and 32 girls; 33 African American, 34 Hispanic, and 1 Asian) of 742 attending camp for the sumnmer. Children scored significantly higher on the measure of self-esteem at the end of camp than at the beginning. Positive descriptions and ratings of self on popularity increased significantly. Observations and interviews with children suggested physical and social environmental features, such as contact with nature and having the same counselor as a previous year, may support self-esteem. Findings are discussed within a framework for biophilia, an innate urge to affiliate with nature which unfolds from earliest childhood on.

  5. CPAFFC Sends Student Delegation On Winter Camp Tour of the U.S.

    Institute of Scientific and Technical Information of China (English)


    <正>Invited by Brigham Young University (BYU)-Hawaii of the U-nited States, a 21-member middle school student delegation, organized by the CPAFFC with the aim of enhancing friendship between the Chinese and American students, made a winter camp tour of the United States from January 29 to February 12.

  6. Child's culture-related experiences with a social robot at diabetes camps

    NARCIS (Netherlands)

    Neerincx, A.; Sacchitelli, F.; Kaptein, R.; Pal, S. van der; Oleari, E.; Neerincx, M.A.


    This paper investigates the experiences of Italian and Dutch children while interacting with a social robot that is designed to support their diabetes self-management. Observations of children's behaviors and analyses of questionnaires at diabetes camps, showed positive experiences with variation (e

  7. Malnourished children in refugee camps and lack of connection with services after US resettlement. (United States)

    Lutfy, Caitlyn; Cookson, Susan T; Talley, Leisel; Rochat, Roger


    Identifying and addressing malnutrition among US-bound refugee children is an important human rights issue. Failure to address childhood malnutrition can impair cognitive development and productivity. The target population was children aged 6-59 months, originating from eight countries representing 51 % of US-resettled refugees for 2005-2011, living in 22 camps prior to potential US-resettlement. The corresponding camp-level nutritional survey data were evaluated. State Refugee Health Coordinators were surveyed on nutritional assessment, reporting and referrals for their US-refugee medical screenings. From 2004 to 2010, half of the camps (63 total surveys) had global acute malnutrition prevalence over 15 % at least once (surveys not done annually) and anemia prevalence greater than 40 %. The majority of US-refugee medical screenings included height and weight measurements but few used national or WHO standards to evaluate presence or level of malnutrition. Improve overseas camp monitoring and link these nutritional data to US-resettling refugee children to inform potential nutritional interventions. Domestically, use WHO or US growth standards for anthropometrics to determine presence of malnutrition and need for corrective action.

  8. Adenyl cyclases and cAMP in plant signaling - Past and present

    KAUST Repository

    Gehring, Christoph A.


    In lower eukaryotes and animals 3\\'-5\\'-cyclic adenosine monophosphate (cAMP) and adenyl cyclases (ACs), enzymes that catalyse the formation of cAMP from ATP, have long been established as key components and second messengers in many signaling pathways. In contrast, in plants, both the presence and biological role of cAMP have been a matter of ongoing debate and some controversy. Here we shall focus firstly on the discovery of cellular cAMP in plants and evidence for a role of this second messenger in plant signal transduction. Secondly, we shall review current evidence of plant ACs, analyse aspects of their domain organisations and the biological roles of candidate molecules. In addition, we shall assess different approaches based on search motifs consisting of functionally assigned amino acids in the catalytic centre of annotated and/or experimentally tested nucleotide cyclases that can contribute to the identification of novel candidate molecules with AC activity such as F-box and TIR proteins. 2010 Gehring; licensee BioMed Central Ltd.

  9. Plant collectors in Ecuador: Camp, Prieto, Giler and Jørgensen

    DEFF Research Database (Denmark)

    Balslev, Henrik; Joyal, Elaine


    The itinerary of W. H. Camp and his assistants in Ecuador has been reconstructed from fieldbooks deposited in the archives of the library at The New York Botanical Garden. The visited localities are listed according to collection numbers, an alphabetical list of the localities with geographical c...

  10. Marketing Your Camp to Diverse Populations: Tips To Reach Ethnic Markets. (United States)

    Nicodemus, Teresa


    Cultural differences enhance the wonder of camp. Tips for reaching diverse populations include emphasizing multicultural programming in promotional materials, distributing flyers in diverse neighborhoods, having diverse staff, advertising on radio stations popular with ethnic communities, offering scholarships, advertising through national ethnic…

  11. Defense Health Care: Activities Related to Past Drinking Water Contamination at Marine Corps Base Camp Lejeune (United States)


    help prevent tooth decay . After the water is treated, it is stored in ground and elevated storage reservoirs. When needed, treated water...service support, and medical support, as well as a Naval Hospital and Naval Dental Center. Base housing at Camp Lejeune consists of enlisted family

  12. Identity Development of Youth during Participation at an Informal Science Education Camp (United States)

    Riedinger, Kelly


    In this exploratory case study, I investigated the ways that youth engaged in negotiating their identity during learning conversations at an informal science education camp. In particular, I was interested in exploring the ways that youth positioned themselves within their learning group and how this influenced their identities as learners of…

  13. Tales from Camp Wilde: Queer(y)ing Environmental Education Research (United States)

    Gough, Noel; Gough, Annette


    This paper questions the relative silence of queer theory and theorizing in environmental education research. We explore some possibilities for queering environmental education research by fabricating (and inviting colleagues to fabricate) stories of Camp Wilde, a fictional location that helps us to expose the facticity of the field's…

  14. Mental Health Outcomes for Youth Living in Refugee Camps: A Review. (United States)

    Vossoughi, Nadia; Jackson, Yo; Gusler, Stephanie; Stone, Katie


    Currently, there are approximately 10.8 million child refugees worldwide. Youth living in refugee camps face a wide range of difficulties placing them at risk for trauma exposure and negative mental health outcomes. However, little is known about the mental health functioning of these youth. The present review provides a systematic review of mental health outcomes for refugee/displaced youth residing in refugee camps. Twenty studies were included in the present review. Among these studies, the prevalence of mental health disorders varied greatly with some studies reporting null effects for disorders like posttraumatic stress disorder and others reporting prevalence as high as 87%. Levels of anxiety, somatic symptoms, depression, and aggression also varied across studies. The results point to the significant need for more research on the mental health of youth residing in refugee camps. Despite the wide range of measurement approaches, the evidence points to a fairly consistent finding of a range of maladjustment problems for youth living in refugee camps. Implications for improving the methodology for investigating mental health are discussed.

  15. 78 FR 41720 - CampCo Petition to Allow Commercial Distribution of Tritium Markers (United States)


    ... rulemaking (PRM) filed with the Commission by CampCo (the petitioner) on December 2, 2011, and supplemented... the public or entering the comment submissions into ADAMS. II. The Petition The NRC received a PRM....802, ``Petition for rulemaking,'' and the petition has been docketed as PRM-32-8. The NRC...

  16. 76 FR 64 - Safety and Health Requirements Related to Camp Cars (United States)


    ...). ``Construction'' includes the `` lacement of a mobile or modular facility,'' which includes placement of a camp... authority, FRA is proposing to amend regulations on construction of employee sleeping quarters. In... right of way of a railroad are within the scope of the prohibition against beginning construction...

  17. Proteomic signatures implicate cAMP in light and temperature responses in Arabidopsis thaliana

    KAUST Repository

    Thomas, Ludivine


    The second messenger 3\\'-5\\'-cyclic adenosine monophosphate (cAMP) and adenylyl cyclases (ACs), enzymes that catalyse the formation of cAMP from ATP, are increasingly recognized as important signaling molecules in a number of physiological responses in higher plants. Here we used proteomics to identify cAMP-dependent protein signatures in Arabidopsis thaliana and identify a number of differentially expressed proteins with a role in light- and temperature-dependent responses, notably photosystem II subunit P-1, plasma membrane associated cation-binding protein and chaperonin 60 β. Based on these proteomics results we conclude that, much like in cyanobacteria, algae and fungi, cAMP may have a role in light signaling and the regulation of photosynthesis as well as responses to temperature and we speculate that ACs could act as light and/or temperature sensors in higher plants. Biological significance: This current study is significant since it presents the first proteomic response to cAMP, a novel and key second messenger in plants. It will be relevant to researchers in plant physiology and in particular those with an interest in second messengers and their role in biotic and abiotic stress responses. © 2013 Elsevier B.V.

  18. Parents' Perceptions of Benefits of Summer Camp for Campers with Mental Retardation. (United States)

    McCormick, Bryan; And Others


    Study explored the perception of parents of campers with mental retardation regarding the benefits of summer camps. Parents rated the relative importance of 22 possible benefits and indicated 6 dimensions of benefits in an ideal program: social skill development, social competence, respite care, cognitive development, expressive development, and…

  19. Post-Secondary Learning Priorities of Workers in an Oil Sands Camp in Northern Alberta (United States)

    Fahy, Patrick J.; Steel, Nancy


    This paper reports results to date of a three-year project by Athabasca University, intended to determine the education and training needs and interests of employees in a work camp in northern Alberta's oil sands. (Future reports will address results of efforts to provide programming suiting the needs identified, and the uptake, satisfaction,…

  20. The central role of cAMP in regulating Plasmodium falciparum merozoite invasion of human erythrocytes.

    Directory of Open Access Journals (Sweden)

    Amrita Dawn


    Full Text Available All pathogenesis and death associated with Plasmodium falciparum malaria is due to parasite-infected erythrocytes. Invasion of erythrocytes by P. falciparum merozoites requires specific interactions between host receptors and parasite ligands that are localized in apical organelles called micronemes. Here, we identify cAMP as a key regulator that triggers the timely secretion of microneme proteins enabling receptor-engagement and invasion. We demonstrate that exposure of merozoites to a low K+ environment, typical of blood plasma, activates a bicarbonate-sensitive cytoplasmic adenylyl cyclase to raise cytosolic cAMP levels and activate protein kinase A, which regulates microneme secretion. We also show that cAMP regulates merozoite cytosolic Ca2+ levels via induction of an Epac pathway and demonstrate that increases in both cAMP and Ca2+ are essential to trigger microneme secretion. Our identification of the different elements in cAMP-dependent signaling pathways that regulate microneme secretion during invasion provides novel targets to inhibit blood stage parasite growth and prevent malaria.

  1. A Behaviorally-Oriented Residential Camping Program for Obese Children and Adolescents. (United States)

    McKenzie, Thomas L.


    Behavioral strategies were used to teach exercise and weight control habits, and were combined with diet planning, nutrition education, and a strenuous physical education program in a residential summer camp, to successfully bring about a significant reduction in body fat and heart disease risk of obese boys, 8-18 years old. (Author/JDD)

  2. Soldier Quality of Life (Operational) and Readiness at Contingency Base Camps: Insights From Qualitative Interviews (United States)


    SOCIAL PSYCHOLOGY MORALE INTERVIEWS GROUP DYNAMICS QOL(QUALITY OF LIFE) SURVEYS...camps and Soldier/small unit readiness to guide the development of a quantitative readiness survey. • During structured interviews, 31 Soldiers...Ensuring optimal conditions for rest, nutrition, and spiritual and psychological support. Executive Summary 4UNCLASSIFIED Background 5UNCLASSIFIED What is

  3. Tianjin Cathy Future Art Troupe Attends International Children’s Summer Camp in DPRK

    Institute of Scientific and Technical Information of China (English)


    <正>The Tianjin Municipal People’s Association for Friendship with Foreign Countries organized and sent a delegation consisting of 15 members of the Cathy Future Children’s Art Troupe to attend the International Children’s Camp in Songdowon,DPRK.

  4. The cAMP Signaling and MAP Kinase Pathways in Plant Pathogenic Fungi

    NARCIS (Netherlands)

    Mehrabi, R.; Zhao, X.; Kim, Y.; Xu, J.R.


    The key components of the well conserved cyclic AMP signaling and MAP kinase pathways have been functionally characterized in the corn smut Ustilago maydis, rice blast fungus Magnaporthe grisea, and a few other fungal pathogens. In general, the cAMP signaling and the MAP kinase cascade homologous to

  5. 75 FR 3212 - Office of Elementary and Secondary Education; College Assistance Migrant Program (CAMP) (United States)


    ... Office of Elementary and Secondary Education; College Assistance Migrant Program (CAMP) Catalog of.... Department of Education, Office of Migrant Education, 400 Maryland Avenue, SW., Room 3E344, Washington, DC.... Department of Education, Office of Migrant Education, 400 Maryland Avenue, SW., room 3E323, Washington,...

  6. Measuring adaptability: psychological examinations of Jewish detainees in Cyprus internment camps. (United States)

    Zalashik, Rakefet; Davidovitch, Nadav


    Two medical delegations, one from Palestine and one from the United States, were sent to detainment camps in Cyprus in the summer of 1947. The British Mandatory government had set up these camps in the summer of 1946 to stem the flow of Jewish immigrants into Palestine after World War II. The purpose of the medical delegations was to screen the camps' inhabitants and to propose a mental-health program for their life in Palestine. We examine the activities of these two delegations within the context of their scientific interest in the psycho-pathology of displaced persons after World War II and as part of a broader project of mental hygiene. According to the delegations, the detainees would be a potential source of strength for building a new society if they adapted to life in Palestine. However, they would become a burden if they failed to be absorbed. At the same time, the medical delegations also saw the detainee camps as a potential "living laboratory" for scientific exploration. The case of the two medical delegations in Cyprus is also a story about constructing and transgressing medical borders. Apart from the obvious fact that this case study deals with movement of people, refugees as well as health-care workers, it is also about the transmission of knowledge and professions across the ocean.

  7. Effects of diazepam on orthodontic tooth movement and alveolar bone cAMP levels in cats. (United States)

    Burrow, S J; Sammon, P J; Tuncay, O C


    Cyclic AMP has been suggested as a possible intracellular mediator in bone remodeling during tooth movement. Accordingly, an increase in the level of this nucleotide should result in faster tooth movement. Breakdown of cAMP was inhibited by administration of diazepam in eight cats undergoing orthodontic tooth movement; another matched group of eight animals served as controls. Orthodontic appliances consisted of coil springs stretching between the right side maxillary and mandibular canines and third premolars. The data for tooth movement and cAMP concentrations were analyzed by repeated measures factorial analyses of variance. The results indicated that administration of diazepam increased the rate of tooth movement at P less than 0.0005 and, interestingly, although diazepam had no effect on undisturbed tissues, it lowered the cAMP levels in the periodontal tissues of orthodontically moved teeth at P less than 0.01. On the basis of these results, it was concluded that the concentration of cAMP did not correlate with bone remodeling in this model and perhaps should not be used as an index of periodontal-tissue response during orthodontic tooth movement.

  8. Results of Kinder Camp: Stovall-Shaw Elementary School, Summer 2001. (United States)

    Irvine, David J.

    North Carolina's Kinder Camp is a 4-week program designed to prepare children for entry into kindergarten. All participants must have completed Project Family Read. A North Carolina elementary school measured the programs effectiveness by using the Cognitive/Language Profile of the American Guidance Service Early Screening Profiles as a pre- and…

  9. Examining Camper Learning Outcomes and Knowledge Retention at Oklahoma FFA Leadership Camp (United States)

    Brown, Nicholas R.; Terry, Robert, Jr.; Kelsey, Kathleen D.


    The National FFA Organization is committed to providing non-formal learning activities focusing on leadership education. Summer camps are a major component of FFA activities and concentrate on personal growth, leadership development, and recreational activities for youth. This repeated measures study determined the level of cognitive gain and the…

  10. Adventure Camp Programs, Self-Concept, and Their Effects on Behavioral Problem Adolescents (United States)

    Larson, Bruce A.


    The purpose of this study was to examine the effects of an adventure camp program on the self-concept of adolescents with behavioral problems. Subjects in the study included 61 randomly selected male and female adolescents ranging in age from 9 to 17 years with behavioral problems. The treatment group of 31 adolescents was randomly selected from a…

  11. Effectiveness of a Therapeutic Summer Camp for Children with ADHD: Phase I Clinical Intervention Trial (United States)

    Hantson, Julie; Wang, Pan Pan; Grizenko-Vida, Michael; Ter-Stepanian, Marina; Harvey, William; Joober, Ridha; Grizenko, Natalie


    Objective: The objective of this study was to evaluate the effectiveness of a 2-week therapeutic summer day camp for children with ADHD, which included a social skills training program and parent psychoeducation and training program. This was an open-label, nonrandomized Phase I Clinical Intervention Trial. Method: Parents completed the Weiss…

  12. Effects of a Developmental Boot Camp: Improving Student Performance on a College Placement Test (United States)

    Hill, Heather H.


    Nationwide, students are entering college unprepared for college-level work. Recent high school graduates are placing into developmental courses at an alarming rate. The purpose of this research study is to examine the effect of a developmental boot camp on standardized placement test scores of students enrolling at a community college in North…

  13. Assessment of Mobilization and Leadership Challenges in Azerbaijani IDP and Refugee Camps. (United States)

    Affolter, Friedrich W.; Findlay, Henry J.


    A study analyzed community mobilization and leadership challenges in Azerbaijan refugee and internally displaced people (IDP) camps. The research determined that there is a lack of capacity to mobilize the community to effective community action and learning. (Contains 21 references.) (Author/JOW)

  14. Developmental regulation and evolution of cAMP signaling in Dictyostelium

    NARCIS (Netherlands)

    Álvarez-Curto, Elisa


    Through evolution the social amoebas have developed mechanisms to adapt to environmental changes and ensure survival. This thesis explores the evolutionary origins of cAMP signalling and regulation of developmental decisions in the model organism Dictyostelium discoideum. It also shows the first mol

  15. Effect of an Engineering Camp on Students' Perceptions of Engineering and Technology (United States)

    Hammack, Rebekah; Ivey, Toni A.; Utley, Juliana; High, Karen A.


    Students' knowledge about a profession influences their future decisions about careers. Research indicates that students tend to hold stereotypical views of engineers, which would hinder engineering as a career choice. The purpose of this study was to measure how participating in a week long engineering summer camp affected middle school students'…

  16. Connecting Out-of-School Learning to Home: Digital Postcards from Summer Camp (United States)

    Zimmerman, Heather Toomey; Gamrat, Christopher; Hooper, Simon


    Parents and children are rapidly adopting mobile technologies, yet designs for mobile devices that serve a communication function to connect parents to children's out-of-school time activities are limited. As a result, our team designed the Digital Postcard Maker so that children attending summer camps can create digital photographs to send…

  17. 75 FR 62755 - Mt. Hood National Forest, Oregon; Cooper Spur-Government Camp Land Exchange (United States)


    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Forest Service Mt. Hood National Forest, Oregon; Cooper Spur-Government Camp Land Exchange AGENCY: Forest... land. The acquisition of the wetlands at Cooper Spur and the easement on the wetlands at...

  18. Lighting the Fire for 25 years: The Nature and Legacy of Astronomy Camp (United States)

    McCarthy, Donald W.; Hooper, E.; Benecchi, S. D.; Henry, T. J.; Kirkpatrick, J. D.; Kulesa, C.; Oey, M. S.; Regester, J.; Schlingman, W. M.; Camp Staff, Astronomy


    In 1988, Astronomy Camp began in an era when science was entirely the realm of professionals, astronomical observatories were off-limits to the public at night, and scientists were not encouraged to spend time in science education. Since then we have grown a dynamic science education program that immerses individuals (ages 11-80), educators, schools, and Girl Scout Leaders in authentic science at Arizona’s research observatories in the Catalina mountains and at Kitt Peak. Often labeled “life changing,” these residential programs have engaged thousands of people from 49 U.S. states and 20 foreign countries. Female enrollment has increased steadily, and women now generally outnumber men in our teenage programs. Graduate students have played a major creative role and many have gone on to become educators and research leaders around the world. By involving a wide range of ages, the Camps have helped strengthen the STEM-pipeline. Many of our alumni remain in touch via social and professional networks and have developed not only into professional astronomers but also into leaders throughout society, parents, and educators. Our emphasis on age-appropriate research helped inspire today’s concepts of research-based science education and Citizen Science. An accompanying paper (E. Hooper et al.) discusses our approach to project-oriented astronomical research. Scientific discoveries include Near-Earth Objects, supernova classification, and lightcurves of Kuiper Belt Objects. The Camps have also contributed to educational research involving informal science education, youth perceptions, and student identities. Ironically, the Camps have leveraged new initiatives in both research and education at NOAO, LSST, and JWST. Here we review the philosophy, conduct, and content of Astronomy Camp and summarize the unexpected nature of its ongoing legacy. We remain grateful to The University of Arizona Alumni Association for its long-term encouragement and support.

  19. cAMP signaling prevents podocyte apoptosis via activation of protein kinase A and mitochondrial fusion.

    Directory of Open Access Journals (Sweden)

    Xiaoying Li

    Full Text Available Our previous in vitro studies suggested that cyclic AMP (cAMP signaling prevents adriamycin (ADR and puromycin aminonucleoside (PAN-induced apoptosis in podocytes. As cAMP is an important second messenger and plays a key role in cell proliferation, differentiation and cytoskeleton formation via protein kinase A (PKA or exchange protein directly activated by cAMP (Epac pathways, we sought to determine the role of PKA or Epac signaling in cAMP-mediated protection of podocytes. In the ADR nephrosis model, we found that forskolin, a selective activator of adenylate cyclase, attenuated albuminuria and improved the expression of podocyte marker WT-1. When podocytes were treated with pCPT-cAMP (a selective cAMP/PKA activator, PKA activation was increased in a time-dependent manner and prevented PAN-induced podocyte loss and caspase 3 activation, as well as a reduction in mitochondrial membrane potential. We found that PAN and ADR resulted in a decrease in Mfn1 expression and mitochondrial fission in podocytes. pCPT-cAMP restored Mfn1 expression in puromycin or ADR-treated podocytes and induced Drp1 phosphorylation, as well as mitochondrial fusion. Treating podocytes with arachidonic acid resulted in mitochondrial fission, podocyte loss and cleaved caspase 3 production. Arachidonic acid abolished the protective effects of pCPT-cAMP on PAN-treated podocytes. Mdivi, a mitochondrial division inhibitor, prevented PAN-induced cleaved caspase 3 production in podocytes. We conclude that activation of cAMP alleviated murine podocyte caused by ADR. PKA signaling resulted in mitochondrial fusion in podocytes, which at least partially mediated the effects of cAMP.

  20. Regulation of melanogenesis: the role of cAMP and MITF

    Directory of Open Access Journals (Sweden)

    Michał Otręba


    Full Text Available The article presents the melanogenesis pathway and the role of cyclic adenosine monophosphate (cAMP and microphthalmia transcription factor (MITF in regulation of this process. Products of melanogenesis are eu- and/or pheomelanins synthesized in a multistage process of tyrosine oxidation and polymerization. The conversions require the presence of tyrosinase (TYR, key enzyme, tyrosine hydroxylase isoform I (THI and tyrosinase related proteins (TRP1 and TRP2. Many types of signal molecules and transcription factors participate in regulation of melanin synthesis, but the most important are cAMP and MITF. cAMP is the second messenger in the intracellular signal cascade, which is synthesized from adenosine triphosphate (ATP by adenylyl cyclase, activated among others by the melanocortin receptor and the αS subunit of G protein. The signal molecule cAMP regulates MITF, TYR, THI, GTP-cyclohydroxylase I (GTP-CHI transcription and phenylalanine hydroxylase (PAH phosphorylation at Ser16 by protein kinase A (PKA. Mutations of genes encoding proteins belonging to the cAMP signal cascade may lead to McCune-Albright and Carney syndromes. MITF is one of the most important nuclear transcription factors regulating melanogenesis. Currently 10 isoforms of human MITF are known, but in melanocytes only MITF-M, MITF-Mdel, MITF-A and MITF-H occur. MITF transcription factor regulates melanogenesis by activation of tyrosinase, TRP1 and TRP2 transcription. It also affects expression of other factors regulating melanosome maturation, biogenesis and transport. Moreover, it regulates melanocyte proliferation and protection against apoptosis. Mutations of the MITF gene may lead to hereditary diseases: Waardenburg type II and Tietz syndromes.

  1. cAMP signaling prevents podocyte apoptosis via activation of protein kinase A and mitochondrial fusion. (United States)

    Li, Xiaoying; Tao, Hua; Xie, Kewei; Ni, Zhaohui; Yan, Yucheng; Wei, Kai; Chuang, Peter Y; He, John Cijiang; Gu, Leyi


    Our previous in vitro studies suggested that cyclic AMP (cAMP) signaling prevents adriamycin (ADR) and puromycin aminonucleoside (PAN)-induced apoptosis in podocytes. As cAMP is an important second messenger and plays a key role in cell proliferation, differentiation and cytoskeleton formation via protein kinase A (PKA) or exchange protein directly activated by cAMP (Epac) pathways, we sought to determine the role of PKA or Epac signaling in cAMP-mediated protection of podocytes. In the ADR nephrosis model, we found that forskolin, a selective activator of adenylate cyclase, attenuated albuminuria and improved the expression of podocyte marker WT-1. When podocytes were treated with pCPT-cAMP (a selective cAMP/PKA activator), PKA activation was increased in a time-dependent manner and prevented PAN-induced podocyte loss and caspase 3 activation, as well as a reduction in mitochondrial membrane potential. We found that PAN and ADR resulted in a decrease in Mfn1 expression and mitochondrial fission in podocytes. pCPT-cAMP restored Mfn1 expression in puromycin or ADR-treated podocytes and induced Drp1 phosphorylation, as well as mitochondrial fusion. Treating podocytes with arachidonic acid resulted in mitochondrial fission, podocyte loss and cleaved caspase 3 production. Arachidonic acid abolished the protective effects of pCPT-cAMP on PAN-treated podocytes. Mdivi, a mitochondrial division inhibitor, prevented PAN-induced cleaved caspase 3 production in podocytes. We conclude that activation of cAMP alleviated murine podocyte caused by ADR. PKA signaling resulted in mitochondrial fusion in podocytes, which at least partially mediated the effects of cAMP.

  2. Phosphodiesterases in the rat ovary: effect of cAMP in primordial follicles. (United States)

    Petersen, Tonny Studsgaard; Stahlhut, Martin; Andersen, Claus Yding


    Phosphodiesterases (PDEs) are important regulators of the intracellular cAMP concentration, which is a central second messenger that affects a multitude of intracellular functions. In the ovaries, cAMP exerts diverse functions, including regulation of ovulation and it has been suggested that augmented cAMP levels stimulate primordial follicle growth. The present study examined the gene expression, enzyme activity and immunolocalization of the different cAMP hydrolysing PDEs families in the rat ovary. Further, the effect of PDE4 inhibition on primordial follicle activation in cultured neonatal rat ovaries was also evaluated. We found varied expression of all eight families in the ovary with Pde7b and Pde8a having the highest expression each accounting for more than 20% of the total PDE mRNA. PDE4 accounted for 15-26% of the total PDE activity. Immunoreactive PDE11A was found in the oocytes and PDE2A in the corpora lutea. Incubating neonatal rat ovaries with PDE4 inhibitors did not increase primordial follicle activation or change the expression of the developing follicle markers Gdf9, Amh, Inha, the proliferation marker Mki67 or the primordial follicle marker Tmeff2. In addition, the cAMP analogue 8-bromo-cAMP did not increase AKT1 or FOXO3A phosphorylation associated with follicle activation or increase the expression of Kitlg known to be associated with follicle differentiation but did increase the Tmeff2, Mki67 and Inha expression in a dose-dependent manner. In conclusion, this study shows that both Pde7b and Pde8a are highly expressed in the rodent ovary and that PDE4 inhibition does not cause an increase in primordial follicle activation.

  3. Biochemical characterization and cellular imaging of a novel, membrane permeable fluorescent cAMP analog

    Directory of Open Access Journals (Sweden)

    Zaccolo Manuela


    Full Text Available Abstract Background A novel fluorescent cAMP analog (8-[Pharos-575]- adenosine-3', 5'-cyclic monophosphate was characterized with respect to its spectral properties, its ability to bind to and activate three main isoenzymes of the cAMP-dependent protein kinase (PKA-Iα, PKA-IIα, PKA-IIβ in vitro, its stability towards phosphodiesterase and its ability to permeate into cultured eukaryotic cells using resonance energy transfer based indicators, and conventional fluorescence imaging. Results The Pharos fluorophore is characterized by a Stokes shift of 42 nm with an absorption maximum at 575 nm and the emission peaking at 617 nm. The quantum yield is 30%. Incubation of the compound to RIIα and RIIβ subunits increases the amplitude of excitation and absorption maxima significantly; no major change was observed with RIα. In vitro binding of the compound to RIα subunit and activation of the PKA-Iα holoenzyme was essentially equivalent to cAMP; RII subunits bound the fluorescent analog up to ten times less efficiently, resulting in about two times reduced apparent activation constants of the holoenzymes compared to cAMP. The cellular uptake of the fluorescent analog was investigated by cAMP indicators. It was estimated that about 7 μM of the fluorescent cAMP analog is available to the indicator after one hour of incubation and that about 600 μM of the compound had to be added to intact cells to half-maximally dissociate a PKA type IIα sensor. Conclusion The novel analog combines good membrane permeability- comparable to 8-Br-cAMP – with superior spectral properties of a modern, red-shifted fluorophore. GFP-tagged regulatory subunits of PKA and the analog co-localized. Furthermore, it is a potent, PDE-resistant activator of PKA-I and -II, suitable for in vitro applications and spatial distribution evaluations in living cells.

  4. RU SciTech: Weaving Astronomy and Physics into a University-sponsored Summer Camp for Middle School Students (United States)

    Hart, Quyen N.


    We present a successful model for organizing a small University-sponsored summer camp that integrates astronomy and physics content with other science disciplines and computer programming content. The aim of our science and technology camp is to engage middle school students in a wide array of critical thinking tasks and hands-on activities centered on science and technology. Additionally, our program seeks to increase and maintain STEM interest among children, particularly in under-represented populations (e.g., Hispanic, African-American, women, and lower socioeconomic individuals) with hopes of decreasing disparities in diversity across many STEM fields.During this four-day camp, organized and facilitated by faculty volunteers, activities rotated through many STEM modules, including optics, telescopes, circuit building, computer hardware, and programming. Specifically, we scaffold camp activities to build upon similar ideas and content if possible. Using knowledge and skills gained through the AAS Astronomy Ambassadors program, we were able to integrate several astronomy activities into the camp, leading students through engaging activities, and conduct educational research. We present best practices on piloting a similar program in a university environment, our efforts to connect the learning outcomes common across all the modules, specifically in astronomy and physics, outline future camp activities, and the survey results on the impact of camp activities on attitudes toward science, technology, and science careers.

  5. The social and cultural life of the prisoners in the Jewish forced labor camp at Skarzysko-Kamienna. (United States)

    Karay, F


    The article discusses the social and cultural life among the Jewish prisoners at the Skarzysko-Kamienna forced labor camp in central Poland. The activities included organized observances of religious and national customs and creative arts and performances of many kinds. The activities served several functions for the artists and audiences. Whether as a source of income in the struggle for physical survival, or as a psychological escape from the terrible reality of daily life in the camp, or as a unifying element for the fragmented prisoner population, or as a from of political resistance, these social and cultural activities in the camp constitute a singular phenomenon in the history of the Holocaust.

  6. gidakiimanaaniwigamig (gidaa) science camps for 3-12 students at Fond du Lac Band of Lake Superior Chippewa (United States)

    Ito, E.; Kowalczak, C.; Dalbotten, D. M.; Pellerin, H.; Greensky, L.


    Gidaa informal science camps have been held in various formats and emphases for over 10 years at Fond du Lac (FDL). These camps provided a safe place for young people to be "nerds" but did not have specific focus. In the last 5 years the camp themes became more focused on environmental issues facing the reservation. From 2009 to 2014 the camps focused on issues surrounding the health of wild rice plants. Since September 2014, we are focusing on climate literacy and how climate changes are affecting tribal environment. We learned from our wild rice themed activities that students are more focused and interested in topics that affect them and their reservation and traditional activities.Each camp has a presentation by a staff from the FDL Resource Management Division. These presentations are sometimes given on Friday evenings after dinner and yet students remain alert, respectful, and actively take notes. The presentation topic is known in advance and camp teachers (local middle and high school teachers who have been with us for 5 to 10 years) plan lessons around that topic. In addition, college students, some of them past camp participants, are on hand to assist the teachers and to provide role models. Students have investigated, for example, possible effects of climate change on the timing of rising of sugar maple sap and whether sugar maple tress will continue to thrive in their area, efforts to bring back sturgeon to local rivers and how the changes in water temperature may affect that effort, possible climate connection to decline of moose and walleye pike population at 5 weekend camps during the last academic year. We have conducted 2 surveys by an outside evaluator and will have the results before the next round of camps starts in fall 2015 to help us tweak the format if necessary. Teachers and planners (PIs) are happy with the way camps are running this year; there is a good balance of camp activities that allow teachers to translate and adapt the

  7. Effects of a recreational physical activity summer camp on body composition, metabolic syndrome and physical fitness in obese children. (United States)

    Roriz DE Oliveira, Mafalda S; Teixeira Seabra, André F; Ribeiro Maia, José A


    This study sought to examine the effects of a recreational physical activity summer camp on body composition, metabolic syndrome, and physical fitness in obese children. Forty-eight children (8-10 years; Body Mass Index ≥85th percentile) completed 4-weeks of a structured recreational physical activity program summer camp (5 hours/day, 5 days/week). Over the 4-weeks, significant reductions (Psummer camp yields several body-composition, metabolic-syndrome, and physical fitness benefits in obese children and should represent an effective support for their health development.

  8. Forskolin's effect on transient K current in nudibranch neurons is not reproduced by cAMP. (United States)

    Coombs, J; Thompson, S


    Forskolin, a diterpene extracted from Coleus forskolii, stimulates the production of cAMP in a variety of cells and is potentially an important tool for studying the role of cAMP in the modulation of neuronal excitability. We studied the effects of forskolin on neurons of nudibranch molluscs and found that it caused characteristic, reversible changes in the amplitude and waveform of the transient K current, IA, and also activated an inward current similar to the cAMP-dependent inward current previously described in molluscan neurons. Forskolin altered the time course of IA activation and inactivation but did not affect the voltage dependence or the reversal potential of the current. IA normally inactivates exponentially, but in forskolin the time course of inactivation can be fit by the sum of 2 exponentials with an initial rate that is faster than the control and a final rate that is much slower. On depolarization in forskolin, IA begins to activate at the normal rate, but a slower component of activation is also seen. The changes in IA in the nudibranch cells were qualitatively different than the changes caused by forskolin in Aplysia bag cell neurons (Strong, 1984). Experiments were performed to determine whether these effects of forskolin require cAMP. Intracellular injection of cAMP, application of membrane-permeable analogs of cAMP, application of phosphodiesterase inhibitors, and intracellular injection of the active catalytic subunit of cAMP-dependent protein kinase did not affect the amplitude or waveform of IA. Also, the changes in IA that are caused by forskolin were not prevented or reversed by intracellular injection of an inhibitor of cAMP-dependent protein kinase. Cyclic AMP did, however, activate inward current at voltages near the resting potential. We conclude that the changes in IA and the activation of inward current represent separate affects of forskolin. The inward current appears to depend on an increase in intracellular cAMP, while the

  9. Writing Intensive Undergraduate Field Camp and Education: Expanding the Classroom and Preparing Students for the Workforce (United States)

    Ford, M. T.; McGehee, T. L.


    There has always been a strong perception within the geoscience community that a capstone field course was the pinnacle of an undergraduate geoscience degree. Such a course draws from the student's accumulated knowledge base, using information from multiple sub-disciplines to solve "real-world" problems. Since 2006, there has been a 92% increase in students attending field camps (Status of the Geoscience Workforce 2014 - AGI). But, the number of field camps has significantly declined. In 1995, 35% of geoscience departments offered a summer field course but by 2006 that number had dropped to 15% (Status Report on Geoscience Summer Field Camps - AGI) and since 2009, the number of field camps listed in the directory has dropped from 100 to about 75. This decline is despite the fact that 88% of industry professionals believe fieldwork should "be an integral and required part of undergraduate programs" (Petcovic, et al., 2014). In 2012, in order to meet the growing needs of industry and better prepare our students, Texas A&M University-Kingsville developed an in-house, unique set of field courses that expand the limits of the classroom. We have two required courses. One is similar to a traditional field camp except that it contains a writing intensive component. The six-credit course runs for seven weeks. Prior to camp, students are required to write an introduction (geologic history section) on the study area. We spend two weeks in the field, mapping daily (Big Bend National Park), and then return to Kingsville. Students then have two weeks to finish a fully referenced paper, including their edited introduction, methods, observations, interpretations, discussion and conclusions and once complete, they begin the introduction for the next area. This is another two-week field session, in central Texas. After this, we return the first paper which has been edited for content by geoscience faculty and for grammar by an English instructor. Students spend the next

  10. Transcriptome changes and cAMP oscillations in an archaeal cell cycle

    Directory of Open Access Journals (Sweden)

    Soppa Jörg


    Full Text Available Abstract Background The cell cycle of all organisms includes mass increase by a factor of two, replication of the genetic material, segregation of the genome to different parts of the cell, and cell division into two daughter cells. It is tightly regulated and typically includes cell cycle-specific oscillations of the levels of transcripts, proteins, protein modifications, and signaling molecules. Until now cell cycle-specific transcriptome changes have been described for four eukaryotic species ranging from yeast to human, but only for two prokaryotic species. Similarly, oscillations of small signaling molecules have been identified in very few eukaryotic species, but not in any prokaryote. Results A synchronization procedure for the archaeon Halobacterium salinarum was optimized, so that nearly 100% of all cells divide in a time interval that is 1/4th of the generation time of exponentially growing cells. The method was used to characterize cell cycle-dependent transcriptome changes using a genome-wide DNA microarray. The transcript levels of 87 genes were found to be cell cycle-regulated, corresponding to 3% of all genes. They could be clustered into seven groups with different transcript level profiles. Cluster-specific sequence motifs were detected around the start of the genes that are predicted to be involved in cell cycle-specific transcriptional regulation. Notably, many cell cycle genes that have oscillating transcript levels in eukaryotes are not regulated on the transcriptional level in H. salinarum. Synchronized cultures were also used to identify putative small signaling molecules. H. salinarum was found to contain a basal cAMP concentration of 200 μM, considerably higher than that of yeast. The cAMP concentration is shortly induced directly prior to and after cell division, and thus cAMP probably is an important signal for cell cycle progression. Conclusion The analysis of cell cycle-specific transcriptome changes of H. salinarum

  11. [Encapsulated voices : Estonian sound recordings from the German prisoner-of-war camps in 1916-1918] / Tõnu Tannberg

    Index Scriptorium Estoniae

    Tannberg, Tõnu, 1961-


    Arvustus: Encapsulated voices : Estonian sound recordings from the German prisoner-of-war camps in 1916-1918 (Das Baltikum in Geschichte und Gegenwart, 5). Hrsg. von Jaan Ross. Böhlau Verlag. Köln, Weimar und Wien 2012

  12. High adenylyl cyclase activity and in vivo cAMP fluctuations in corals suggest central physiological role. (United States)

    Barott, K L; Helman, Y; Haramaty, L; Barron, M E; Hess, K C; Buck, J; Levin, L R; Tresguerres, M


    Corals are an ecologically and evolutionarily significant group, providing the framework for coral reef biodiversity while representing one of the most basal of metazoan phyla. However, little is known about fundamental signaling pathways in corals. Here we investigate the dynamics of cAMP, a conserved signaling molecule that can regulate virtually every physiological process. Bioinformatics revealed corals have both transmembrane and soluble adenylyl cyclases (AC). Endogenous cAMP levels in live corals followed a potential diel cycle, as they were higher during the day compared to the middle of the night. Coral homogenates exhibited some of the highest cAMP production rates ever to be recorded in any organism; this activity was inhibited by calcium ions and stimulated by bicarbonate. In contrast, zooxanthellae or mucus had >1000-fold lower AC activity. These results suggest that cAMP is an important regulator of coral physiology, especially in response to light, acid/base disturbances and inorganic carbon levels.

  13. Digital Geologic Map of the American Camp Unit and vicinity, Washington (NPS, GRD, GRE, SAJH, SJIS digital map) (United States)

    National Park Service, Department of the Interior — The Digital Geologic Map of the American Camp Unit and vicinity, Washington is composed of GIS data layers complete with ArcMap 9.2 layer (.LYR) files, two ancillary...

  14. The Trumpeter, Company 424 Civilian Conservation Corps Camp Redington in Swan Quarter, North Carolina; October Issue, Volume 1 (United States)

    US Fish and Wildlife Service, Department of the Interior — This document is a historic monthly report of Camp Redington Civilian Conservation Corps located in Swan Quarter, North Carolina. It describes the progress and...

  15. Children and Youth Camp Safety Act, 1978. Hearing Before the Subcommittee on Child and Human Development of the Committee on Human Resources, United States Senate, 95th Congress, 2nd Session on S. 258--To Provide for the Development and Implementation of Programs for Children and Youth Camp Safety (March 21, 1978). (United States)

    Congress of the U.S., Washington, DC. Senate Committee on Human Resources.

    The product of some 10 years of work directed toward federal legislation addressing and defining youth camp safety, the Youth Camp Safety Act (S. 258), as presented in these hearings, calls for the federal government to assume a role in the development of state health and safety standards for children attending youth camps in any state in the…

  16. IL-4 induces cAMP and cGMP in human monocytic cells

    Directory of Open Access Journals (Sweden)

    B. Dugas


    Full Text Available Human monocytes, preincubated with IFN-γ respond to IL-4 by a cGMP increase through activation of an inducible NO synthase. Here, IL-4 was found to induce an accumulation of cGMP (1 – 3 min and cAMP (20 – 25 min in unstimulated monocytes. This was impaired with NOS inhibitors, but also with EGTA and calcium/calmodulin inhibitors. These results suggest that: (1 IL-4 may stimulate different NOS isoforms in resting and IFN-γ activated monocytes, and (2 cAMP accumulation may be partially dependent on the NO pathway. By RT-PCR, a type III constitutive NOS mRNA was detected in U937 monocytic cells. IL-4 also increased the [Ca2+]i in these cells. Different NOS may thus be expressed in monocytic cells depending on their differentiation and the signals they receive.

  17. Narratives from Jenin Refugee Camp: Children as extreme defence against the disintegration of family and community

    Directory of Open Access Journals (Sweden)

    Guido Veronese


    Full Text Available This paper aim to explore practices that create serious risks to the physical and psychological  health of Palestinian children. The typical stories of three children interviewed at Jenin Refugee Camp are subjected to content analysis. This analysis also extends to the micro and macro social developmental context of these children (which the share with the entire population of the camp. Key themes emerging from the analysis include the need to "redeem" grand parents and parents (depressed, preoccupied, without hope, intolerance of imprisonment and being coerced into confined spaces, which are invaded on daily bases, the need to spaces to play in, the acceleration, through violence, toward adulthood. The motivations leading to dangerous practices and risk and protective factors are discussed.

  18. H.E. Mr Josep Piqué i Camps, Minister of Science and Technology, Spain

    CERN Multimedia

    Patrice Loïez


    Photo 01: H.E. Mr Josep Piqué i Camps, Minister for Science and Technology, Spain (right foreground) visiting SM 18 with (from left to right) F. Rodriguez Mateos, G. Babé, C. Dopazo in front of H.E. Mr J. Pérez-Villanueva y Tovar, Ambassador. Photo 05: The delegation, accompanied by Spanish scientists at CERN, also visited the LHC superconducting magnet test hall. From l. to r.: Matteo Cavalli-Sforza of CERN, Josep Piqué i Camps, Spanish Minister of Science and Technology, César Dopazo, Director-General of CIEMAT (Spanish Research Centre for Energy, Environment and Technology), Juan Antonio Rubio, ETT Division Leader at CERN, Manuel Aguilar-Benitez, Spanish Delegate to Council, Gonzalo León, Secretary-General of Scientific Policy to the Minister, and Manuel Delfino, IT Division Leader at CERN.

  19. Ultraviolet light induction of skin carcinoma in the mouse; influence of cAMP modifying agents. (United States)

    Zajdela, F; Latarjet, R


    A short review of pathogenic factors in U.V. light skin carcinogenesis in the mouse is presented. Caffeine and theophylline applied locally during U.V. irradiation caused a 50 percent reduction of skin tumour induction in Swiss mice. These two chemicals are inhibitors of DNA postreplication repair, but they also raise the intracellular level of cyclic AMP by inhibiting cAMP phosphodiesterase with, as a consequence, a possible slowing down of cellular growth. Control experiments using three different chemicals capable of raising the cAMP level in epidermal cells gave negative results. These experimental data are compatible with our original hypothesis according to which production of skin cancers by U.V. radiation is in same way related to DNA repair which helps the cell to survive but allows or favours the occurrence of errors in cellular DNA.

  20. An innovative summer camp program improves weight and self-esteem in obese children. (United States)

    Wong, William W; Abrams, Stephanie H; Mikhail, Carmen; Terrazas, Norma L; Wilson, Theresa A; Arceo, Diana; Mrowczynski, Paula K; King, Kristi L; Stansel, Amanda D; Albright, Ashley N; Barlow, Sarah E; Brown, Kimberly O; Brown, Jason D; Klish, William J


    To determine the potential benefits of a residential summer camp to treat childhood obesity, 21 obese, multiethnic children (aged 11.4+/-1.4 years; body mass index [BMI] percentile 98.5+/-1.4; BMI z score 2.30+/-0.33) from a diverse socioeconomic background were enrolled in a 2-week summer camp program. Significant improvements (P<0.04) were observed in self-esteem (+0.27+/-0.33 point), body weight (-3.7+/-1.2 kg), BMI (-1.60+/-0.48 kg/m), BMI z score (-0.12+/-0.06), number of curl ups (+10.9+/-21.5), systolic and diastolic blood pressure (-10.8+/-13.4 and -9.4+/-5.5 mmHg, respectively), and heart rate (-8.2+/-12.7 bpm).

  1. Spatial and verbal memory test scores following yoga and fine arts camps for school children. (United States)

    Manjunath, N K; Telles, Shirley


    The performance scores of children (aged 11 to 16 years) in verbal and spatial memory tests were compared for two groups (n = 30, each), one attending a yoga camp and the other a fine arts camp. Both groups were assessed on the memory tasks initially and after ten days of their respective interventions. A control group (n = 30) was similarly studied to assess the test-retest effect. At the final assessment the yoga group showed a significant increase of 43% in spatial memory scores (Multivariate analysis, Tukey test), while the fine arts and control groups showed no change. The results suggest that yoga practice, including physical postures, yoga breathing, meditation and guided relaxation improved delayed recall of spatial information.

  2. Narratives from Jenin Refugee Camp: Children as extreme defence against the disintegration of family and community

    Directory of Open Access Journals (Sweden)

    Guido Veronese


    Full Text Available This paper aim to explore practices that create serious risks to the physical and psychological health of Palestinian children. The typical stories of three children interviewed at Jenin Refugee Camp are subjected to content analysis. This analysis also extends to the micro and macro social developmental context of these children (which the share with the entire population of the camp. Key themes emerging from the analysis include the need to "redeem" grand parents and parents (depressed, preoccupied, without hope, intolerance of imprisonment and being coerced into confined spaces, which are invaded on daily bases, the need to spaces to play in, the acceleration, through violence, toward adulthood. The motivations leading to dangerous practices and risk and protective factors are discussed.

  3. Camping the gothic: que(e)ring sexuality in Truman Capote's Other Voices, Other Rooms. (United States)

    Mitchell-Peters, B


    Since its release in the late 1940s, Other Voices, Other Rooms has remained an arguably unpopular novella in the works of Truman Capote; its criticism is thus far from recent. Most critiques explore the work in its historical milieu of Southern-gothic fiction, either intentionally or unintentionally avoiding the very prominent queer themes. This article acknowledges Capote's use of gothic paradigms and the text's process of undermining gothic motifs to highlight its two adolescent queer characters. Moreover, the text's own Camp discourse is the liberating force that extinguishes the looming Southern-gothic background to expose the sexual possibilities for its young characters. Amidst the sea of late forties and fifties fiction that frequently ensconced the death of the homosexual character, this novella serves as an exception: through a humorous Camp aesthetic, the text gives birth to its inherent queer desires.

  4. Associative conditioning analog selectively increases cAMP levels of tail sensory neurons in Aplysia. (United States)

    Ocorr, K A; Walters, E T; Byrne, J H


    Bilateral clusters of sensory neurons in the pleural ganglia of Aplysia contain cells involved in a defensive tail withdrawal reflex. These cells exhibit heterosynaptic facilitation in response to noxious skin stimulation that can be mimicked by the application of serotonin. Recently it has been shown that this facilitation can be selectively amplified by the application of a classical conditioning procedure to individual sensory neurons. We now report that an analog of this classical conditioning paradigm produces a selective amplification of the cAMP content of isolated sensory neuron clusters. The enhancement is achieved within a single trial and appears to be localized to the sensory neurons. These results indicate that a pairing-specific enhancement of cAMP levels may be a biochemical mechanism for associative neuronal modifications and perhaps learning.

  5. The Role of Camp in Promoting the Participants’ Spoken English Expression

    Directory of Open Access Journals (Sweden)

    jalaluddin Jalaluddin


    Full Text Available The study investigated the topics of participants’ spoken expressionin an English camp and how the topics were discussed.A case study was applied as the research design. Data were gained from focus-group interviews, observation, and questionnaire. The results showed that the participants talked about various topics, which could be categorized into two types i.e. guided topics and situational topics. Guided topics were discussed by the participants in guided conditions. On the other hand, situational topics appeared naturally with respect to the situation. The data also indicated that the activeness and confidence of the participants to talk in English gradually increasedduring the English camp. The findings suggested that English campsbeheld regularly as they can boost the participants’ English speaking skill.

  6. Propionibacterium acnes CAMP factor and host acid sphingomyelinase contribute to bacterial virulence: potential targets for inflammatory acne treatment.

    Directory of Open Access Journals (Sweden)

    Teruaki Nakatsuji

    Full Text Available BACKGROUND: In the progression of acne vulgaris, the disruption of follicular epithelia by an over-growth of Propionibacterium acnes (P. acnes permits the bacteria to spread and become in contact with various skin and immune cells. METHODOLOGY/PRINCIPAL FINDINGS: We have demonstrated in the present study that the Christie, Atkins, Munch-Peterson (CAMP factor of P. acnes is a secretory protein with co-hemolytic activity with sphingomyelinase that can confer cytotoxicity to HaCaT keratinocytes and RAW264.7 macrophages. The CAMP factor from bacteria and acid sphingomyelinase (ASMase from the host cells were simultaneously present in the culture supernatant only when the cells were co-cultured with P. acnes. Either anti-CAMP factor serum or desipramine, a selective ASMase inhibitor, significantly abrogated the P. acnes-induced cell death of HaCaT and RAW264.7 cells. Intradermal injection of ICR mouse ears with live P. acnes induced considerable ear inflammation, macrophage infiltration, and an increase in cellular soluble ASMase. Suppression of ASMase by systemic treatment with desipramine significantly reduced inflammatory reaction induced by intradermal injection with P. acnes, suggesting the contribution of host ASMase in P. acnes-induced inflammatory reaction in vivo. Vaccination of mice with CAMP factor elicited a protective immunity against P. acnes-induced ear inflammation, indicating the involvement of CAMP factor in P. acnes-induced inflammation. Most notably, suppression of both bacterial CAMP factor and host ASMase using vaccination and specific antibody injection, respectively, cooperatively alleviated P. acnes-induced inflammation. CONCLUSIONS/SIGNIFICANCE: These findings envision a novel infectious mechanism by which P. acnes CAMP factor may hijack host ASMase to amplify bacterial virulence to degrade and invade host cells. This work has identified both CAMP factor and ASMase as potential molecular targets for the development of drugs

  7. Campsites as Utopias? A Socio-spatial Reading of the Postwar Holiday Camp in Belgium, 1950s to 1970s


    Janina Gosseye; Hilde Heynen


    This paper contrasts the objectives that underlie the development of government-funded holiday camps for family vacations in Belgium with the socio-spatial practices of their initial users. Drawing on oral history, archival material, photographs and site plans, we argue that holidaymakers did not just experience the holiday camp as an environment where they could reconnect with their family and pursue authentic experiences in close contact with nature – as their initiators had intended – but ...

  8. Abundance, Species Richness, and Reproductive Success of Tidal Marsh Birds at China Camp State Park, Marin County, California


    Wood, Julian K.; Liu, Leonard; Nur, Nadav; Herzog, Mark; Warnock, Nils


    Extensive habitat loss and degradation have resulted in decreases in populations of tidal marsh breeding birds in the San Francisco Estuary in the past 150 years. We conducted point count surveys and nest monitoring in tidal marsh habitat at China Camp State Park from 1996 through 2007 to assess bird abundance, species richness and reproductive success over time. We found overall species richness at China Camp to be significantly lower than that of other San Pablo Bay tidal marshes, but also ...

  9. Ca2+ participates in α1B-adrenoceptor-mediated cAMP response in HEK293 cells

    Institute of Scientific and Technical Information of China (English)

    Yao SONG; Yun-fang LI; Er-dan DONG; Qi-de HAN; You-yi ZHANG


    Aim: To investigate the α1B-adrenoceptor (α1B-AR)-mediated cAMP response and underlying mechanisms in HEK293 cells. Methods: Full-length cDNA encoding α1B-AR was transfected into HEK293 cells using the calcium phosphate precipitation method, and α1B-AR expression and cAMP accumulation were determined by using the saturation radioligand binding assay and ion-exchange chromatography, respectively. Results: Under agonist stimulation, α1B-AR mediated cAMP synthesis in HEK293 cells, and blockade by PLC-PKC or tyrosine kinase did not reduce cAMP accumulation induced by NE. Pretreatment with pertussis toxin(PTX) had little effect on basal cAMP accumulation as well as norepinephrine(NE)-stimulated cAMP accumulation. In addition, pretreatment with cholera toxin(CTX) neither mimicked nor blocked the effect induced by NE. The extracellular Ca2+ chelator egtazic acid (EGTA), nonselective Ca2+ channel blocker CdC12 and calmodulin (CaM) inhibitor W-7 significantly reduced NE-induced cAMP accumulation from 1.59%±0.47% to 1.00%±0.31%, 0.78%±0.23%, and 0.90%±0.40%,respectively. Conclusion: By coupling with a PTX-insensitive G protein, α1B-AR promotes Ca2+ influx via receptor-dependent Ca2+ channels, then Ca2+ is linked to CaM to form a Ca2+-CaM complex, which stimulates adenylyl cyclase (AC),thereby increasing the cAMP production in HEK293 cell lines.

  10. Evaluation of the NOAA CAREERS Weather Camp's Effectiveness in Promoting Atmospheric Science amongst High School Students (United States)

    Olgin, J. G.; Fitzgerald, R. M.; Morris, V. R.


    The NOAA Center for Atmospheric Science (NCAS) sponsors the Channeling Atmospheric Research into Educational Experiences Reaching Students program (CAREERS); a program that manages a network of weather camps for students in secondary education with particular focus on increasing access for students from traditionally underrepresented backgrounds. Hosted by a college or university, the primary mission goals of the program are to engage students in discussions, lectures and interactive projects to better learn and comprehend a suite of atmospheric science disciplines (i.e. weather forecasting, environmental modeling, atmospheric data acquisition), and guide talented students towards higher education to pursue careers in atmospheric science primarily, or toward other STEM field professions. The need to evaluate and analyze the program's efficacy is crucial for continued growth and sustainability. Therefore a means to identify and measure the success of the program's initiatives will be addressed. Two Hispanic serving institutions, the University of Texas at El Paso (UTEP) and the University of Puerto Rico in Mayaguez (UPRM), both hosted the CAREER weather camps during the summers of 2012 and 2013, and provide the basis of this initial analysis. Participants performed entrance surveys of their knowledge of atmospheric science prior to the course. They were then re-evaluated through exit surveys over the topics covered during the weather camp. These data will be analyzed to correlate which program activities worked best in increasing participant awareness (i.e. geology tours of the local area, discussion on local climate variations, geophysical and geochemical demonstrations), and comprehension of atmospheric science. A comparison between the two universities on their uniqueness in program design and execution will also highlight those activities that best progressed CAREERS' program goals. Results from this analysis, along with possible new strategies for improved

  11. Effect of an Engineering Camp on Students’ Perceptions of Engineering and Technology


    Hammack, Rebekah; Ivey, Toni A; Utley, Juliana; High, Karen A


    Students’ knowledge about a profession influences their future decisions about careers. Research indicates that students tend to hold stereotypical views of engineers, which would hinder engineering as a career choice. The purpose of this study was to measure how participating in a week long engineering summer camp affected middle school students’ (N519) attitudes towards engineering and their conceptions of engineering and technology. Results indicate that participation in the programs had a...

  12. Stochastic noise and synchronisation during Dictyostelium aggregation make cAMP oscillations robust


    Kim, J


    Author Summary The molecular network, which underlies the oscillations in the concentration of adenosine 3′, 5′-cyclic monophosphate (cAMP) during the aggregation phase of starvation-induced development in Dictyostelium discoideum, achieves remarkable levels of robust performance in the face of environmental variations and cellular heterogeneity. However, the reasons for this robustness remain poorly understood. Tools and concepts from the field of control engineering provide powerful methods...

  13. Assessing Operation Purple: A Program Evaluation of a Summer Camp for Military Youth (United States)


    caregivers, respectively. Spouses of deployed servicemembers are also at risk for depression and other emotional problems (Mansfield et al., 2010...communities (Goodwin and Staples, 2005). Meltzer and Rourke (2005) examined social comparisons among adolescents at a summer camp for cancer patients...Parental Depression ,” Journal of Family Psychology, Vol. 21, No. 4, December 2007, pp. 703–713. Bers, Marina Umaschi, and Clement Chau, “Fostering Civic

  14. Grow Together under the Sunshine——The 4th Summer Camp for AIDS Orphaned Children

    Institute of Scientific and Technical Information of China (English)



    @@ The 4th summer camp for AIDS-orphaned children was held in Beijing from August 2-8,2007.Co-sponsored by the China Youth Concern Commission and China Foundation for the Prevention and Control of STDs (sexually transmitted disease) and AIDS,the event was attended by 80 AIDS-orphaned children aged 8-16 from 18 counties and cities of 8 provinces of Shanxi,Yunnan,Henan,Jilin,Liaoning,Guizhou,Sichuan and Anhui.

  15. Adsorption/Desorption Measurements of Nitroglycerin and Dinitrotoluene in Camp Edwards, Massachusetts Soil (United States)


    Figure 1. Fate-and-transport conceptual model for the deposition of fired propellant ing nitrocellulose , nitroglycerin, and dinitrotoluene...Double-base propellants used within newer small-arms ammunition typi- cally contain up to 84% nitrocellulose (NC), with 10% nitroglycerin (NG), a... modifier , and flash suppressant in gun propellants . Consequently, they have been identified at the SAR and gun and mortar firing points at Camp

  16. The microbiologic quality of drinking water in North Carolina migrant labor camps. (United States)

    Ciesielski, S; Handzel, T; Sobsey, M


    A two-year study of the microbiological quality of drinking water in 27 randomly selected North Carolina migrant labor camps yielded total and fecal coliform prevalences of 44 percent and 26 percent, respectively in 1988 and similar but higher prevalences in 1989. Preoccupancy testing by county sanitarians had found virtually no total coliform contamination. These findings suggest that a potential source of contamination existed and that current testing protocols which rely on preoccupancy testing may be inadequate.

  17. FimL regulates cAMP synthesis in Pseudomonas aeruginosa.

    Directory of Open Access Journals (Sweden)

    Yuki F Inclan

    Full Text Available Pseudomonas aeruginosa, a ubiquitous bacteria found in diverse ecological niches, is an important cause of acute infections in immunocompromised individuals and chronic infections in patients with Cystic Fibrosis. One signaling molecule required for the coordinate regulation of virulence factors associated with acute infections is 3', 5'-cyclic adenosine monophosphate, (cAMP, which binds to and activates a catabolite repressor homolog, Vfr. Vfr controls the transcription of many virulence factors, including those associated with Type IV pili (TFP, the Type III secretion system (T3SS, the Type II secretion system, flagellar-mediated motility, and quorum sensing systems. We previously identified FimL, a protein with histidine phosphotransfer-like domains, as a regulator of Vfr-dependent processes, including TFP-dependent motility and T3SS function. In this study, we carried out genetic and physiologic studies to further define the mechanism of action of FimL. Through a genetic screen designed to identify suppressors of FimL, we found a putative cAMP-specific phosphodiesterase (CpdA, suggesting that FimL regulates cAMP levels. Inactivation of CpdA increases cAMP levels and restores TFP-dependent motility and T3SS function to fimL mutants, consistent with in vivo phosphodiesterase activity. By constructing combinations of double and triple mutants in the two adenylate cyclase genes (cyaA and cyaB, fimL, and cpdA, we show that ΔfimL mutants resemble ΔcyaB mutants in TM defects, decreased T3SS transcription, and decreased cAMP levels. Similar to some of the virulence factors that they regulate, we demonstrate that CyaB and FimL are polarly localized. These results reveal new complexities in the regulation of diverse virulence pathways associated with acute P. aeruginosa infections.

  18. Reaching the unreached: Mobile surgical camps in a remote village of Himachal Pradesh

    Directory of Open Access Journals (Sweden)

    Sonal Bathla


    Conclusion: Uterovaginal prolapse is not only socially embarrassing and disabling; its surgical treatment is complex and costly too. The free mobile surgical camps under "Project Prolapse" in Shillai, Himachal Pradesh has provided relief to old neglected, disabled women suffering from prolapse in this remote village. Parallel counseling of women and dais for safe hospital delivery and training subordinates in prolapse surgery may help in addressing the problem of POP in this area in the long run.

  19. Retinoic acid and cAMP inhibit rat hepatocellular carcinoma cell proliferation and enhance cell differentiation

    Energy Technology Data Exchange (ETDEWEB)

    Ionta, M. [Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Alfenas MG (Brazil); Departamento de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo SP (Brazil); Rosa, M.C.; Almeida, R.B.; Freitas, V.M.; Rezende-Teixeira, P.; Machado-Santelli, G.M. [Departamento de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo SP (Brazil)


    Hepatocellular carcinoma (HCC) is the third highest cause of cancer death worldwide. In general, the disease is diagnosed at an advanced stage when potentially curative therapies are no longer feasible. For this reason, it is very important to develop new therapeutic approaches. Retinoic acid (RA) is a natural derivative of vitamin A that regulates important biological processes including cell proliferation and differentiation. In vitro studies have shown that RA is effective in inhibiting growth of HCC cells; however, responsiveness to treatment varies among different HCC cell lines. The objective of the present study was to determine if the combined use of RA (0.1 µM) and cAMP (1 mM), an important second messenger, improves the responsiveness of HCC cells to RA treatment. We evaluated the proliferative behavior of an HCC cell line (HTC) and the expression profile of genes related to cancer signaling pathway (ERK and GSK-3β) and liver differentiation [E-cadherin, connexin 26 (Cx26), and connexin 32 (Cx32)]. RA and cAMP were effective in inhibiting the proliferation of HTC cells independently of combined use. However, when a mixture of RA and cAMP was used, the signals concerning the degree of cell differentiation were increased. As demonstrated by Western blot, the treatment increased E-cadherin, Cx26, Cx32 and Ser9-GSK-3β (inactive form) expression while the expression of Cx43, Tyr216-GSK-3β (active form) and phosphorylated ERK decreased. Furthermore, telomerase activity was inhibited along treatment. Taken together, the results showed that the combined use of RA and cAMP is more effective in inducing differentiation of HTC cells.

  20. Cartographies of the Political Camp of Afro-Descendents in Latin America

    Directory of Open Access Journals (Sweden)

    Agustín Lao-Montes


    Full Text Available This article lays out, in general terms, what it calls the political camp of Afro-descendents in Latin America. After establishing a series of theoretical and methodological criteria for the historical analysis of black movements in modernity and the Afro-American movements in particular, the article focuses on the emergence of afro-descendant movements in Latin America during the last part of the 1980s. One of the principal arguments is that in the 1990s a political camp of afro-descendents starts to emerge in the region of Latin America based on a series of developments, including the emergence of new social movements that included ethno-racial movements of Afros and indigenous people, events of regional importance like the contra-celebration of 1492 in 1992, the World Conference against Racism 2001 in Durban, South Africa, and the effects of the neoliberal pattern of globalization. The political camp of Afro-descendents is composed not only of social movements, but also of state actors and transnational actors (such as the World Bank and the Ford Foundation. The article concludes with an analysis of the challenges and perspectives of Afro-American politics in general and of Afro-Latin movements in particular considering the current crisis of the modern/colonial world-system.

  1. Seizure Suppression by High Temperature via cAMP Modulation in Drosophila (United States)

    Saras, Arunesh; Tanouye, Mark A.


    Bang-sensitive (BS) Drosophila mutants display characteristic seizure-like activity (SLA) and paralysis after mechanical shock . After high-frequency electrical stimulation (HFS) of the brain, they generate robust seizures at very low threshold voltage. Here we report an important phenomenon, which effectively suppresses SLA in BS mutants. High temperature causes seizure suppression in all BS mutants (parabss1, eas, sda) examined in this study. This effect is fully reversible and flies show complete recovery from BS paralysis once the temperature effect is nullified. High temperature induces an increase in seizure threshold after a brief pulse of heat shock (HS). By genetic screening, we identified the involvement of cAMP in the suppression of seizures by high temperature. We propose that HS induces adenylyl cyclase which in turn increases cAMP concentration which eventually suppresses seizures in mutant flies. In summary, we describe an unusual phenomenon, where high temperature can suppress SLA in flies by modulating cAMP concentration. PMID:27558668

  2. Looking Outside: Summer Camp for Children Who Have Undergone a Solid-Organ Transplant. (United States)

    Bilhartz, Jacob L; Drayton, Amy K; Shieck, Victoria L


    A high-quality critical care team is an essential component of any successful organ transplant program. From pretransplant care to the crucial postoperative period, its importance cannot be discounted. However, because of the focused nature of work in an intensive care unit (ICU), all too often members of the ICU team are not able to see and appreciate the ultimate fruits of their labor. These are factors that can contribute to the high rates of burnout and turnover among ICU teams. This article presents the concept of a summer camp for children who have received a solid-organ transplant. We discuss a vehicle by which ICU staff as well as other members of a patient's care team can gain a better appreciation of the full nature of both medical and, perhaps more importantly in this situation, nonmedical goals and outcomes in pediatric transplantation. We review our institutional experience running a summer camp for these children, discuss some of the important points in making such a camp successful, and discuss potential benefits to the campers as well as those taking care of them.

  3. [Evaluation of metabolic control in a summer camp for diabetic children (Villarcayo 1991)]. (United States)

    Fuertes Alija, J J; Ruiz Pérez, E; Galindo Jimeno, M; Ergueta Martín, P; González Sarmiento, E


    The relevance of the diabetological education in the treatment of diabetis mellitus must be a prioritary goal in order to reach a proper metabolic control and to avoid as much as possible both acute metabolic complications and long-term vascular and neurological complications, achieving thus a total integration of the diabetic patient in the society. We have monitored during 15 days a group of diabetic children with IDDM in a summer camp, assessing in all of them several clinical and biochemical parameters. The results have been statistically analyzed using the Student's test. After the stay in the summer camp, we observed a significant reduction in the total dose of insulin (p < 0.001) and in the number of hypoglycemias (p < 0.001) and an increase in the average levels of capillary glycemia at the end of the study period when compared with the beginning, with p < 0.001 (breakfast preprandial), p < 0.05 (lunch preprandial) and p < 0.01 (dinner and night preprandial). We did not observe any significant differences in the other parameters studied. In inclusion, the stay in the summer camp of this group had a positive effect, achieving a better metabolic control with lower doses of insulin.

  4. The Effect of an Altitude Training Camp on Swimming Start Time and Loaded Squat Jump Performance. (United States)

    García-Ramos, Amador; Štirn, Igor; Padial, Paulino; Argüelles-Cienfuegos, Javier; De la Fuente, Blanca; Calderón, Carmen; Bonitch-Góngora, Juan; Tomazin, Katja; Strumbelj, Boro; Strojnik, Vojko; Feriche, Belén


    This study evaluated the influence of an altitude training (AT) camp on swimming start time and loaded squat jump performance. To accomplish this goal, 13 international swimmers (8 women, 5 men) were allocated to both the control (Sea Level Training, SLT) and experimental conditions (AT, 2320 m above sea level) that were separated by a one year period. All tests (15 m freestyle swimming start and loaded squat jumps with additional loads of 25%, 50%, 75%, and 100% of swimmers' body weight) were performed before and after a concurrent 3-week strength and endurance training program prescribed by the national coach. Following the SLT camp, significant impairments in swimming start times to 10 (+3.1%) and 15 m (+4.0%) were observed (P camp (-0.89%; P > 0.05). Trivial changes in peak velocity were obtained during the loaded squat jump after both training periods (effect sizes: effectiveness of power-oriented resistance training in the development of explosive actions.

  5. Diatom acclimation to elevated CO2 via cAMP signalling and coordinated gene expression (United States)

    Hennon, Gwenn M. M.; Ashworth, Justin; Groussman, Ryan D.; Berthiaume, Chris; Morales, Rhonda L.; Baliga, Nitin S.; Orellana, Mónica V.; Armbrust, E. V.


    Diatoms are responsible for ~40% of marine primary productivity, fuelling the oceanic carbon cycle and contributing to natural carbon sequestration in the deep ocean. Diatoms rely on energetically expensive carbon concentrating mechanisms (CCMs) to fix carbon efficiently at modern levels of CO2 (refs , , ). How diatoms may respond over the short and long term to rising atmospheric CO2 remains an open question. Here we use nitrate-limited chemostats to show that the model diatom Thalassiosira pseudonana rapidly responds to increasing CO2 by differentially expressing gene clusters that regulate transcription and chromosome folding, and subsequently reduces transcription of photosynthesis and respiration gene clusters under steady-state elevated CO2. These results suggest that exposure to elevated CO2 first causes a shift in regulation, and then a metabolic rearrangement. Genes in one CO2-responsive cluster included CCM and photorespiration genes that share a putative cAMP-responsive cis-regulatory sequence, implying these genes are co-regulated in response to CO2, with cAMP as an intermediate messenger. We verified cAMP-induced downregulation of CCM gene δ-CA3 in nutrient-replete diatom cultures by inhibiting the hydrolysis of cAMP. These results indicate an important role for cAMP in downregulating CCM and photorespiration genes under elevated CO2 and provide insights into mechanisms of diatom acclimation in response to climate change.

  6. Dermatologic challenges of health care for displaced people. lessons from a German emergency refugee camp

    Directory of Open Access Journals (Sweden)

    Uwe Wollina


    Full Text Available Background: The World faces the highest waves of displaced people since World War II. There is limited knowledge about need of dermatological care for refugees and asylum seekers. Methods: We report the experience with a temporary emergency refugee camp in Dresden form the viewpoint of a hospital department. This is a descriptive report covering the period of 10 weeks. Results: In this refugee camp up to 1 100 people were hosted. The male to female ratio was 5.3. The majority of inhabitants were young males (60%, 20% were children. While 40% of refuges came from Syria, Afghanistan, Iraq and Pakistan were also important countries of origin. Communication war a crucial issue while providing health care. Dermatologic service was granted as consultation, outpatient and inpatient clinic. Most contacts were noted in the outpatient clinic. The majority of patient attended the clinic with communicable diseases such as bacterial or viral infections and infestations. Wounds and chronic inflammatory diseases were rather uncommon. Only 4 patients had to be treated in the hospital (inpatient clinic. Conclusions: Displaced people (refugees, asylum seekers come in big waves to Europe. Dermatologic service is an important part of first aid health care in an emergency camp. Language barriers and cultural barriers have to overcome for optimal service. This is the first report from Germany.

  7. A refugee camp in the centre of Europe: clinical characteristics of asylum seekers arriving in Brussels (United States)

    van Berlaer, Gerlant; Bohle Carbonell, Francisca; Manantsoa, Sofie; de Béthune, Xavier; Buyl, Ronald; Debacker, Michel; Hubloue, Ives


    Background In the summer of 2015, the exodus of Syrian war refugees and saturation of refugee camps in neighbouring countries led to the influx of asylum-seekers in European countries, including Belgium. This study aims to describe the demographic and clinical characteristics of asylum seekers who arrived in a huddled refugee camp, in the centre of a well-developed country with all medical facilities. Methods Using a descriptive cross-sectional study design, physicians of Médecins du Monde prospectively registered age, gender, origin, medical symptoms and diagnoses of all patients presenting to an erected field hospital in Brussels in September 2015. Diagnoses were post hoc categorised according to the International Classification of Diseases. Results Of 4037 patients examined in the field hospital, 3907 were included and analysed for this study. Over 11% of patients suffered from injuries, but these were outnumbered by the proportion of patients with respiratory (36%), dental (9%), skin (9%) and digestive (8%) diagnoses. More than 49% had features of infections at the time of the consultation. Conclusions Asylum seekers arriving in a refugee camp in Brussels after a long and hazardous journey suffer mostly from respiratory, dental, skin and digestive diseases. Still, one in seven suffers from injury. These findings, consistent with other reports, should be anticipated when composing emergency medical teams and interagency emergency health or similar kits to be used in a field hospital, even in a Western European country. Trial registration number ISRCTN13523620, Results. PMID:27884856

  8. Controlling fertilization and cAMP signaling in sperm by optogenetics. (United States)

    Jansen, Vera; Alvarez, Luis; Balbach, Melanie; Strünker, Timo; Hegemann, Peter; Kaupp, U Benjamin; Wachten, Dagmar


    Optogenetics is a powerful technique to control cellular activity by light. The light-gated Channelrhodopsin has been widely used to study and manipulate neuronal activity in vivo, whereas optogenetic control of second messengers in vivo has not been examined in depth. In this study, we present a transgenic mouse model expressing a photoactivated adenylyl cyclase (bPAC) in sperm. In transgenic sperm, bPAC mimics the action of the endogenous soluble adenylyl cyclase (SACY) that is required for motility and fertilization: light-stimulation rapidly elevates cAMP, accelerates the flagellar beat, and, thereby, changes swimming behavior of sperm. Furthermore, bPAC replaces endogenous adenylyl cyclase activity. In mutant sperm lacking the bicarbonate-stimulated SACY activity, bPAC restored motility after light-stimulation and, thereby, enabled sperm to fertilize oocytes in vitro. We show that optogenetic control of cAMP in vivo allows to non-invasively study cAMP signaling, to control behaviors of single cells, and to restore a fundamental biological process such as fertilization.

  9. The nature and impact of chronic stressors on refugee children in Ban Mai Nai Soi camp, Thailand. (United States)

    Meyer, Sarah; Murray, Laura K; Puffer, Eve S; Larsen, Jillian; Bolton, Paul


    Refugee camps are replete with risk factors for mental health problems among children, including poverty, disruption of family structure, family violence and food insecurity. This study, focused on refugee children from Burma, in Ban Mai Nai Soi camp in Thailand, sought to identify the particular risks children are exposed to in this context, and the impacts on their mental health and psychosocial well-being. This study employed two qualitative methods--free list interviews and key informant interviews--to identify the main problems impacting children in Ban Mai Nai Soi camp and to explore the causes of these problems and their impact on children's well-being. Respondents in free list interviews identified a number of problems that impact children in this context, including fighting between adults, alcohol use by adults and children, and child abuse and neglect. Across the issues, the causes included economic and social conditions associated with living in the camp and changes in family structures. Children are chronically exposed to stressors during their growth and development in the camp environment. Policies and interventions in areas of protracted displacement in camp-based settings should work to address these stressors and their impacts at community, household and individual levels.

  10. Altered beta-adrenergic receptor-stimulated cAMP formation in cultured skin fibroblasts from Alzheimer donors. (United States)

    Huang, H M; Gibson, G E


    An alteration in signal transduction systems in Alzheimer's disease would likely be of pathophysiological significance, because these steps are critical to normal brain function. Since dynamic processes are difficult to study in autopsied brain, the current studies utilized cultured skin fibroblasts. The beta-adrenergic-stimulated increase in cAMP was reduced approximately 80% in fibroblasts from Alzheimer's disease compared with age-matched controls. The deficit in Alzheimer fibroblasts in response to various adrenergic agonists paralleled their beta-adrenergic potency, and enhancement of cAMP accumulation by a non-adrenergic agonist, such as prostaglandin E1, was similar in Alzheimer and control fibroblasts. Diminished adenylate cyclase activity did not underlie these abnormalities, since direct stimulation of adenylate cyclase by forskolin elevated cAMP production equally in Alzheimer and control fibroblasts. Cholera toxin equally stimulated cAMP formation in Alzheimer and control fibroblasts. Moreover, cholera toxin partially reduced isoproterenol-induced cAMP deficit in Alzheimer fibroblasts. Pertussis toxin, on the other hand, did not alter the Alzheimer deficits. The results suggest either that the coupling of the GTP-binding protein(s) to the beta-adrenergic receptor is abnormal or that the sensitivity of receptor is altered with Alzheimer's disease. Further, any hypothesis about Alzheimer's disease must explain why a reduced beta-adrenergic-stimulated cAMP formation persists in tissue culture.

  11. cAMP effects in neuroendocrine tumors: The role of Epac and PKA in cell proliferation and adhesion. (United States)

    Vitali, E; Cambiaghi, V; Spada, A; Tresoldi, A; Zerbi, A; Peverelli, E; Carnaghi, C; Mantovani, G; Lania, A G


    cAMP effects have been initially attributed to protein kinase A (PKA) activation. Subsequently, two exchange proteins directly activated by cAMP (Epac1/2) have been identified as cAMP targets. Aim of this study was to investigate cAMP effects in pancreatic-NET (P-NET) and bronchial carcinoids and in corresponding cell lines (QGP-1 and H727) on cell proliferation and adhesion and to determine PKA and Epac role in mediating these effects. We found that cAMP increased cyclin D1 expression in P-NET and QGP-1 cells, whereas it had opposite effects on bronchial carcinoids and H727 cells and it promoted cell adhesion in QGP-1 and H727 cells. These effects are mimicked by Epac and PKA specific analogs, activating the small GTPase Rap1. In conclusion, we demonstrated that cAMP exerted divergent effects on proliferation and promoted cell adhesion of different neuroendocrine cell types, these effects being mediated by both Epac and PKA and involving the same effector GTPase Rap1.

  12. Effect of cAMP on short-circuit current in isolated human ciliary body

    Institute of Scientific and Technical Information of China (English)

    WU Ren-yi; MA Ning; HU Qian-qian


    Background Cyclic adenosine monophosphate (cAMP) could activate chloride channels in bovine ciliary body and trigger an increase in the ionic current (short-circuit current,Isc) across the ciliary processes in pigs.The purpose of this study was to investigate how cAMP modulates Isc in isolated human ciliary processes and the possible involvement of chloride transport across the tissue in cAMP-induced Isc change.Methods In an Ussing-type chamber system,the Isc changes induced by the cAMP analogue 8-bromo-cAMP and an adenylyl cyclase activator forskolin in isolated human ciliary processes were assessed.The involvement of Cl-component in the bath solution was investigated.The effect of Cl-channel (10 μmol/L niflumic acid and 1 mmol/L 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid (DIDS)),K+ channel (10 mmol/L tetraethylammonium chloride (TEA)),or Na+ channel blockers (1 mmol/L amiloride) on 8-bromo-cAMP-induced Isc change was also studied.Results Dose-dependently,8-bromo-cAMP (10 nmol/L-30 μmol/L) or forskolin (10 nmol/L-3 μmol/L) increased Isc across the ciliary processes with an increase in negative potential difference on the non-pigmented epithelium (NPE) side of the tissue.Isc increase induced by 8-bromo-cAMP was more pronounced when the drug was applied on the NPE side than on the pigmented epithelium side.When the tissue was bathed in low Cl-solutions,the Isc increase was significantly inhibited.Finally,niflumic acid and DIDS,but not TEA or amiloride,significantly prevented the Isc increase induced by 8-bromo-cAMP.Conclusions cAMP stimulates stroma-to-aqueous anionic transport in isolated human ciliary processes.Chloride is likely to be among the ions,the transportation of which across the tissue is triggered by cAMP,suggesting the potential role of cAMP in the process of aqueous humor formation in human eyes.

  13. Pleiotropic effects of cAMP on germination, antibiotic biosynthesis and morphological development in Streptomyces coelicolor. (United States)

    Süsstrunk, U; Pidoux, J; Taubert, S; Ullmann, A; Thompson, C J


    In wild-type Streptomyces coelicolor MT1110 cultures, cyclic adenosine 3',5' monophosphate (cAMP) was synthesized throughout the developmental programme with peaks of accumulation both during germination and later when aerial mycelium and actinorhodin were being produced. Construction and characterization of an adenylate cyclase disruption mutant (BZ1) demonstrated that cAMP facilitated these developmental processes. Although pulse-labelling experiments showed that a similar germination process was initiated in BZ1 and MT1110, germ-tube emergence was severely delayed in BZ1 and never occurred in more than 85% of the spores. Studies of growth and development on solid glucose minimal medium (SMMS, buffered or unbuffered) showed that MT1110 and BZ1 produced acid during the first rapid growth phase, which generated substrate mycelium. Thereafter, on unbuffered SMMS, only MT1110 resumed growth and produced aerial mycelium by switching to an alternative metabolism that neutralized its medium, probably by reincorporating and metabolizing extracellular acids. BZ1 was not able to neutralize its medium or produce aerial mycelium on unbuffered SMMS; these defects were suppressed by high concentrations (>1 mM) of cAMP during early growth or on buffered medium. Other developmental mutants (bldA, bldB, bldC, bldD, bldG) also irreversibly acidified this medium. However, these bald mutants were not suppressed by exogenous cAMP or neutralizing buffer. BZ1 also differentiated when it was cultured in close proximity to MT1110, a property observed in cross-feeding experiments between bald mutants and commonly thought to reflect diffusion of a discrete positively acting signalling molecule. In this case, MT1110 generated a more neutral pH environment that allowed BZ1 to reinitiate growth and form aerial mycelium. The fact that actinorhodin synthesis could be induced by concentrations of cAMP (< 20 microM) found in the medium of MT1110 cultures, suggested that it may serve as a

  14. Preliminary Outcomes from a Week-Long Environmental Engineering Summer Camp for High School Female Students (United States)

    Balasubramanian, S.; Koloutsou-Vakakis, S.


    There is a need for environment engineers and sustainability managers to address global environmental, energy and health challenges. Environmental literacy programs at K-12 level provide a unique opportunity in motivating young minds in joining STEM and also provide additional value in learning about "saving planet earth". The Women in Engineering at the University of Illinois organize an annual week long camp, for female high school students with tracks corresponding to different fields of Engineering. The Environmental Engineering and Sustainability (EES) track is organized by faculty and graduate students of the Civil and Environmental Engineering department and introduces students to concepts in sustainability and systems thinking in connection with air and water quality, climate change and renewable energy. This study is a preliminary assessment of the relevance of the EES outreach track conducted in July 2014 in student learning. Specific goals include assessing (a) demographics of participants and their motivation to join this camp, (b) educational and enjoyability quotients of the modules and (c) learning and motivational outcomes using the Likert scale. A pre-camp survey indicated keen interest in learning about environmental engineering (4.56/5.0) and expected this camp to be a venue to learn about related career choices (4.9/5.0). Five days of instruction were divided thematically and included a mix of lectures, activity based learning, demonstrations and field visits. Overall modules were rated as educational (4.4/5.0) and enjoyable (4.5/5.0). Modules with hands-on learning were best received (4.67/5.0) and rated unique (4.7/5.0). Post camp, participants acknowledged the important contribution of environmental engineers to society (4.8/5.0) and could relate the different modules to the role engineer's play (4.06/5.0) for sustainability. On an average, the participants evinced interest in engineering as a career choice (4.0/5.0) but there was a broader

  15. Traditional Geology Field Camp: A capstone course at South Dakota School of Mines and Technology (BHNSFS) (United States)

    Uzunlar, N.; Lisenbee, A. L.


    The Black Hills Natural Sciences Field Station (BHNSFS) has provided field training in geology and geological engineering for more than 40 years, and since the 1980's as a consortium serving five schools with South Dakota School of Mines and Technology as the coordinator. The traditional summer geology field camp is a five week long, intense program aimed to prepare students for subsequent professional geologic experiences. It is delivered from two separate facilities, one in the Black Hills (South Dakota) from a beautiful log lodge along Sand Creek, in eastern Wyoming, and a second from the town of Taskesti along the North Anatolian fault approximately 200 km east of Istanbul, Turkey. At both locations, the courses maintain a strong emphasis on basic field applications, including the use of GPS as a mapping tool in most exercises. The preparation of well-written reports, based on field descriptions supplemented by research on the web or through published documents, is strongly emphasized. Projects at the Black Hills field camp includes mapping of Precambrian basement, Paleozoic stratigraphy, and Laramide Tertiary plutons and structural features as welll as post-Laramide,, faulted continental strata. The popular Taskesti field camp utilizes the diverse geology of the Tethyan realm, as well as the culture and history, of central Turkey (Anatolia). The course is based at a Turkish Government Earthquake Research Center facility along the North Anatolian fault. Students examine and map selected locations across the Izmir-Ankara suture including: 1) Deformed Cretaceous and Tertiary carbonate and clastic strata of the Sakarya micro-continent in a fore-arc basin; 2) Marble and skarn surrounding Eocene, subduction-related granite intruded into a passive margin sequence in the Sivrihisar region of central Anatolia; 3) Faulted and folded Neogene strata in the northern flank of the post-Tethyan, Haymana Basin and the contrasting terrains across the North Anatolian fault (J

  16. Ethics Camp: The Appropriate Management Model for Northeastern Region Youth, Ecclesiastical Region 9

    Directory of Open Access Journals (Sweden)

    Wongsathit Wisupee


    Full Text Available Problem statement: Youth’s ethics tended to be a continuous increased problem and they were in their age without judgment. Youths were very important for national development in future, if their problems were not urgently solved it would overall effect to way of live in their families, communities, socials and Thailand. Approach: This research aimed to study backgrounds, current conditions, problems on management and the appropriate management model of Ethics Camp for the Northeastern Region Youth, Ecclesiastical Region 9. The study was a qualitative research conducted in 4 provinces 1 place of the Northeastern Region Youth, Ecclesiastical Region 9 and Thailand. Observation form, survey form, interview form, focus group discussion and study shop were used as research tools. Triangulation was used to test all data and data was analyzed by descriptive research. Results: The result founded that the background in overall of 4 places were began by making study trips of directors or lecturing monks at famous camp and they, later, brought those models back to establish ethics camps in their own provinces. For current conditions and problems, it was found that; in aspect of personnel-there was lacking of competent personnel, in aspect of budget-there was lacking of budget for expenses, in aspect of material-some place had old and damaged equipments, in aspect of management-there was not enough budget for long-term training and also lacking of lecturing monks, in aspect of marketing-there was lacking of public relation and distinct documents of courses and in aspect of technological innovation-lecturing monks had no knowledge to produce modern technological media. For appropriate management model, it was found that; in aspect of personnel-camp directors should have wide and long vision, intelligence, perseverance, honesty and human relation while lecturing monks must be professional; in aspect of budget-charges for any expenses should be depended on

  17. Reconstructive Surgery Camp for Leprosy Deformities in a Tertiary Hospital: An Example of Service Delivery at Low Costs (United States)

    Bhatt, Nikita R.; Vyas, Kinnari; Merchant, Rajiv


    Introduction Seventy percent of all cases of leprosy in the world occur in India. 8,462 new cases of disability were reported in India between 2010-11. Reconstructive Surgery Camps (RSC) provide free of cost plastic surgical expertise to patients of leprosy with deformity. Aim The aim of this article was to report the outcomes of a RSC in a tertiary level university hospital in India. We also described the types of deformities in the patient group and the cost of conducting such a camp. Materials and Methods The RSC involved 130 patients with leprosy related deformities operated by a team of plastic surgeons in a tertiary university hospital over 5 days. Health workers of the National Leprosy Elimination Program identified patients at community level. The camp was funded by the central government of India and the patients were provided incentives for undergoing treatment. Results Plantar ulcer was the commonest deformity (51.5%) while lagopthalmos (9.2%) was the least common deformity in the patient group. The overall complication rate in our study was around 10.6% (n=11). The total cost of this camp was 730,000 rupees (£7029.9). Conclusion Reconstructive surgery in a camp setup is a low cost alternative of correcting leprosy related deformity. It also provides valuable practical experience in reconstructive surgery to surgical trainees. Tertiary hospital based camps for conducting large-scale surgeries may be a cost effective alternative to reduce waiting lists in public health sectors. Long-term studies monitoring patients operated in a camp setting would be worthwhile. PMID:27630868

  18. Contos de Camp Wilde: tornando queer a pesquisa em educação ambiental Tales from Camp Wilde: queer(ying environmental education research

    Directory of Open Access Journals (Sweden)

    Noel Gough


    Full Text Available Este artigo questiona o relativo silêncio da teoria e da teorização queer sobre a pesquisa em educação ambiental. Exploramos algumas possibilidades para tornar queer a pesquisa em educação ambiental ao criar (estimulando outras/os a fazer o mesmo narrativas de Camp Wilde, um local imaginário que nos ajuda a expor o fato de este ser um campo marcado por uma construção heteronormativa. Essas narrativas propõem métodos alternativos de representação e (reprodução do sujeito e do objeto de nossas indagações e nossas identidades como pesquisadoras/es. As/os colaboradoras/es utilizam-se de diferentes recursos teóricos como história da arte, desconstrução, ecofeminismo, crítica literária, estudos culturais populares e pós-estruturalismo feminista a fim de desenvolver uma nova orientação para a pesquisa em Educação Ambiental, a qual esperamos que jamais seja categorizada como um 'novo gênero'.This paper questions the relative silence of queer theory and theorizing in environmental education research. We explore some possibilities for queering environmental education research by fabricating (and inviting colleagues to fabricate stories of Camp Wilde, a fictional location that helps usto expose the facticity of the field's heteronormative constructedness. These stories suggest alternative ways of (representing and (reproducing both the subjects/objects of our inquiries and our identities as researchers. The contributors draw on a variety of theoretical resources from art history, deconstruction, ecofeminism, literary criticism, popular cultural studies, and feminist poststructuralism to perform an orientation to environmental education research that we hope will never be arrested by its categorization as a "new genre."

  19. Le voyage au service d’une peinture de la France et des Français : Maxime Du Camp en Hollande

    Directory of Open Access Journals (Sweden)

    Rozanne Versendaal


    Full Text Available This article aims to study the travelogue En Hollande (1859, written by the French author and photographer Maxime Du Camp. This travel document deals with a two weeks journey that Du Camp made in Holland in the winter of 1857. The article particularly examines the way Du Camp has painted the picture of France and the French people, while travelling through different regions of the Netherlands. The article presents in detail some cultural, religious and political aspects of the French identity and culture Du Camp reflects upon in his text.

  20. The Geothermal Field Camp: Capacity building for geothermal energy systems in Indonesia (United States)

    Moeck, I.; Sule, R.; Saptadji, N. M.; Deon, F.; Herdianita, N. R.; Jolie, E.; Suryantini, N.; Erbas, K.


    In July 2011, the first geothermal field camp was hold on Java/Indonesia near the city Bandung south of the volcanic field Tangkuban Perahu. The course was organized by the Institut Teknologie Bandung (ITB) and International Centre for Geothermal Research (ICGR) of the German Centre of Geosciences (GFZ). The purpose of the Geothermal Field Camp is to combine both field based work and laboratory analysis to ultimately better understand the data collected in field and to integrate data gained by various disciplines. The training belongs to a capacity building program for geothermal energy systems in Indonesia and initially aims to train the trainers. In a later stage, the educational personal trained by the Geothermal Field Camp shall be able to hold their individual Geothermal Field Camp. This is of special interest for Indonesia where the multitude of islands hindered a broad uniform education in geothermal energy systems. However, Indonesia hold the largest geothermal potential worldwide and educated personal is necessary to successfully develop this huge potential scattered over region in future. The interdisciplinary and integrative approach combined with field based and laboratory methodologies is the guiding principle of the Geothermal Field Camp. Tangkuban Perahu was selected because this field allows the integration of field based structural geological analysis, observation and sampling of geothermal manifestations as hot springs and sinters and ultimately of structural geology and surface geochemistry. This innovative training introduces in methods used in exploration geology to study both, fault and fracture systems and fluid chemistry to better understand the selective fluid flow along certain fractures and faults. Field geology covered the systematic measurement of faults and fractures, fault plane and fracture population analysis. In addition, field hydro-geochemistry focused on sampling techniques and field measurements onsite. Subsequent data analysis

  1. Impact of an informal learning science camp on urban, low socioeconomic status middle school students and participating teacher-leaders (United States)

    Votaw, Nikki L.

    Studies suggest that students have difficulty connecting science to their own lives (Lee & Fradd, 1998; Aikenhead, 1996). This difficulty results in a decline in students' attitudes toward science, leading to low science achievement. These factors result in fewer students interested in careers related to science, specifically for urban, minority students. The purpose of this study was to examine the impact that a ten day informal learning immersion science camp had on the participants, both urban, low-socioeconomic status middle school students and teacher-leaders. The students were incoming seventh grade students involved in a community-based scholar program designed to recruit and support socioeconomically disadvantaged, academically talented students. The teacher-leaders were professional educators working toward an advanced degree. This ten day camp included seven visits to different sites and complementary classroom-based activities. The purpose of the camp was to immerse the students in informal learning environments that affect their daily lives. Students and teacher-leaders visited facilities that provide public utility services (i.e. power plant, sewage treatment facility, and water company), zoo, large commercial cave system, planetarium, university based electrooptics and nanotechnology center, and forest and arboretum. These site visits were supported by activities that were provided by teacher-leaders. A model used as a framework for studying learning in the context of this ten day camp as Falk and Dierking's (2000) Contextual Model for Learning. This model described three basic intersecting elements that contributed to learning within the given context. The three contexts (personal, sociocultural, and physical) intersect affecting the learning that takes place. A mixed methodology design was employed to determine the impact of the camp on students' content knowledge and attitudes toward science. Qualitative data were collected to determine the impact

  2. -Adrenergic receptors on rat ventricular myocytes: characteristics and linkage to cAMP metabolism

    Energy Technology Data Exchange (ETDEWEB)

    Buxton, I.L.O.; Brunton, L.L.


    When incubated with purified cardiomyocytes from adult rat ventricle, the 1-antagonist (TH)prazosin binds to a single class of sites with high affinity. Competition for (TH)prazosin binding by the 2-selective antagonist yohimbine and the nonselective -antagonist phentolamine demonstrates that these receptors are of the 1-subtype. In addition, incubation of myocyte membranes with (TH)yohimbine results in no measurable specific binding. Agonist competition for (TH)prazosin binding to membranes prepared from purified myocytes demonstrates the presence of two components of binding: 28% of 1-receptors interact with norepinephrine with high affinity (K/sub D/ = 36 nM), whereas the majority of receptors (72%) have a low affinity for agonist (K/sub D/ = 2.2 M). After addition of 10 M GTP, norepinephrine competes for (TH)prazosin binding to a single class of sites with lower affinity (K/sub D/ = 2.2 M). Incubation of intact myocytes for 2 min with 1 M norepinephrine leads to significantly less cyclic AMP (cAMP) accumulation than stimulation with either norepinephrine plus prazosin or isoproterenol. Likewise, incubation of intact myocytes with 10 W M norepinephrine leads to significantly less activation of cAMP-dependent protein kinase than when myocytes are stimulated by both norepinephrine and the 1-adrenergic antagonist, prazosin or the US -adrenergic agonist, isoproterenol. They conclude that the cardiomyocyte 1 receptor is coupled to a guanine nucleotide-binding protein, that 1-receptors are functionally linked to decreased intracellular cAMP content, and that this change in cellular cAMP is expressed as described activation of cAMP-dependent protein kinase.

  3. Developing knowledge intensive ideas in engineering education: the application of camp methodology (United States)

    Heidemann Lassen, Astrid; Løwe Nielsen, Suna


    Background: Globalization, technological advancement, environmental problems, etc. challenge organizations not just to consider cost-effectiveness, but also to develop new ideas in order to build competitive advantages. Hence, methods to deliberately enhance creativity and facilitate its processes of development must also play a central role in engineering education. However, so far the engineering education literature provides little attention to the important discussion of how to develop knowledge intensive ideas based on creativity methods and concepts. Purpose: The purpose of this article is to investigate how to design creative camps from which knowledge intensive ideas can unfold. Design/method/sample: A framework on integration of creativity and knowledge intensity is first developed, and then tested through the planning, execution and evaluation of a specialized creativity camp with focus on supply chain management. Detailed documentation of the learning processes of the participating 49 engineering and business students is developed through repeated interviews during the process as well as a survey. Results: The research illustrates the process of development of ideas, and how the participants through interdisciplinary collaboration, cognitive flexibility and joint ownership develop highly innovative and knowledge-intensive ideas, with direct relevance for the four companies whose problems they address. Conclusions: The article demonstrates how the creativity camp methodology holds the potential of combining advanced academic knowledge and creativity, to produce knowledge intensive ideas, when the design is based on ideas of experiential learning as well as creativity principles. This makes the method a highly relevant learning approach for engineering students in the search for skills to both develop and implement innovative ideas.

  4. Point prevalence of pseudoexfoliation syndrome in patients scheduled for cataract surgery in eye camps in Yemen

    Directory of Open Access Journals (Sweden)

    Al-Shaer Mutahar


    Full Text Available Purpose: To study the point prevalence of pseudoexfoliation syndrome (PXS among Yemeni patients in different governorates with age-related cataract scheduled for surgery. Settings: Eye camps organized by the Nibras Health Society to perform cataract surgeries during the years 2002-2006. All patients aged 40 years and above were included in the study. Materials and Methods: A total of 2535 eyes of 2535 patients from 13 governorates, scheduled for cataract surgery in eye camps, were included. All eyes underwent complete eye examination before the surgery and were evaluated for the signs of pseudoexfoliation material in the pupil, iris and lens capsule on dilated slit lamp examination. Results: The study found 495 of the 2535 eyes (19.53% with PXS with males more commonly affected than females (55.2 and 44.8%, respectively. The mean age of patients with PXS was 66.2 years while it was 64.6 years in non-PXS patients. The prevalence of pseudoexfoliation syndrome increased with age (10.1% in the age group of 41-50 years that increased to 28.8% in the age group of more than 81 years old. The rate of PXS detection in camps in 13 governorates ranged from 13.33 to 24.22% with an overall rate of 19.53%. The lowest rate was noticed in Sana′a and the highest in Al-Dhale governorate. Conclusion: This pilot study confirms that PXS was common in patients undergoing cataract surgery in Yemen with an increased detection rate with age. This study also highlights the prevalence of an ocular disease that is associated with systemic and ocular complications; however, further studies based on population studies are needed.

  5. Dibutyryl cAMP effects on thromboxane and leukotriene production in decompression-induced lung injury (United States)

    Little, T. M.; Butler, B. D.


    Decompression-induced venous bubble formation has been linked to increased neutrophil counts, endothelial cell injury, release of vasoactive eicosanoids, and increased vascular membrane permeability. These actions may account for inflammatory responses and edema formation. Increasing the intracellular cAMP has been shown to decrease eicosanoid production and edema formation in various models of lung injury. Reduction of decompression-induced inflammatory responses was evaluated in decompressed rats pretreated with saline (controls) or dibutyryl cAMP (DBcAMP, an analog of cAMP). After pretreatment, rats were exposed to either 616 kPa for 120 min or 683 kPa for 60 min. The observed increases in extravascular lung water ratios (pulmonary edema), bronchoalveolar lavage, and pleural protein in the saline control group (683 kPa) were not evident with DBcAMP treatment. DBcAMP pretreatment effects were also seen with the white blood cell counts and the percent of neutrophils in the bronchoalveolar lavage. Urinary levels of thromboxane B2, 11-dehydrothromboxane B2, and leukotriene E4 were significantly increased with the 683 kPa saline control decompression exposure. DBcAMP reduced the decompression-induced leukotriene E4 production in the urine. Plasma levels of thromboxane B2, 11-dehydrothromboxane B2, and leukotriene E4 were increased with the 683-kPa exposure groups. DBcAMP treatment did not affect these changes. The 11-dehydrothromboxane B2 and leukotriene E4 levels in the bronchoalveolar lavage were increased with the 683 kPa exposure and were reduced with the DBcAMP treatment. Our results indicate that DBcAMP has the capability to reduce eicosanoid production and limit membrane permeability and subsequent edema formation in rats experiencing decompression sickness.

  6. Training Diaries during Altitude Training Camp in Two Olympic Champions: An Observational Case Study. (United States)

    Pugliese, Lorenzo; Serpiello, Fabio R; Millet, Grégoire P; La Torre, Antonio


    Traditionally, Live High-Train High (LHTH) interventions were adopted when athletes trained and lived at altitude to try maximising the benefits offered by hypoxic exposure and improving sea level performance. Nevertheless, scientific research has proposed that the possible benefits of hypoxia would be offset by the inability to maintain high training intensity at altitude. However, elite athletes have been rarely recruited as an experimental sample, and training intensity has almost never been monitored during altitude research. This case study is an attempt to provide a practical example of successful LHTH interventions in two Olympic gold medal athletes. Training diaries were collected and total training volumes, volumes at different intensities, and sea level performance recorded before, during and after a 3-week LHTH camp. Both athletes successfully completed the LHTH camp (2090 m) maintaining similar absolute training intensity and training volume at high-intensity (> 91% of race pace) compared to sea level. After the LHTH intervention both athletes obtained enhancements in performance and they won an Olympic gold medal. In our opinion, LHTH interventions can be used as a simple, yet effective, method to maintain absolute, and improve relative training intensity in elite endurance athletes. Key PointsElite endurance athletes, with extensive altitude training experience, can maintain similar absolute intensity during LHTH compared to sea level.LHTH may be considered as an effective method to increase relative training intensity while maintaining the same running/walking pace, with possible beneficial effects on sea level performance.Training intensity could be the key factor for successful high-level LHTH camp.

  7. Preliminary assessment report for Camp Carroll Training Center, Installation 02045, Anchorage, Alaska. Installation Restoration Program

    Energy Technology Data Exchange (ETDEWEB)

    Krokosz, M.; Sefano, J.


    This report presents the results of the preliminary assessment (PA) conducted by Argonne National Laboratory at the Alaska Army National Guard property known as Camp Carroll Training Center, located on the Fort Richardson Army facility near Anchorage, Alaska. Preliminary assessments of federal facilities are being conducted to compile the information necessary for the completion of preremedial activities and to provide a basis for establishing, corrective actions in response to releases of hazardous substances. The principal objective of the PA is to characterize the site accurately and determine the need for further action by examining site activities, types and quantities of hazardous substances used, the nature and amounts of wastes generated or stored at the facility, and potential pathways by which contamination could affect public health and the environment. The primary environmentally significant operations (ESOs) associated with the property are (1) the Alaska Air National Guard storage area behind Building S57112 (Organizational Maintenance Shop [OMS] 6); (2) the state of Alaska maintenance facility and the soil/tar-type spill north of the state of Alaska maintenance facility; (3) the waste storage area adjacent to OMS 6; (4) the contaminated area from leaking underground storage tanks (USTs) and the oil-water separator; and (5) soil staining in the parking area at the Camp Carroll Headquarters Building. Camp Carroll appears to be in excellent condition from an environmental standpoint, and current practices are satisfactory. Argonne recommends that the Alaska Department of Military Affairs consider remediation of soil contamination associated with all storage areas, as well as reviewing the practices of other residents of the facility. Argonne also recommends that the current methods of storing waste material behind Building S57112 (OMS 6) be reviewed for alternatives.

  8. Camps 2.0: exploring the sequence and structure space of prokaryotic, eukaryotic, and viral membrane proteins. (United States)

    Neumann, Sindy; Hartmann, Holger; Martin-Galiano, Antonio J; Fuchs, Angelika; Frishman, Dmitrij


    Structural bioinformatics of membrane proteins is still in its infancy, and the picture of their fold space is only beginning to emerge. Because only a handful of three-dimensional structures are available, sequence comparison and structure prediction remain the main tools for investigating sequence-structure relationships in membrane protein families. Here we present a comprehensive analysis of the structural families corresponding to α-helical membrane proteins with at least three transmembrane helices. The new version of our CAMPS database (CAMPS 2.0) covers nearly 1300 eukaryotic, prokaryotic, and viral genomes. Using an advanced classification procedure, which is based on high-order hidden Markov models and considers both sequence similarity as well as the number of transmembrane helices and loop lengths, we identified 1353 structurally homogeneous clusters roughly corresponding to membrane protein folds. Only 53 clusters are associated with experimentally determined three-dimensional structures, and for these clusters CAMPS is in reasonable agreement with structure-based classification approaches such as SCOP and CATH. We therefore estimate that ∼1300 structures would need to be determined to provide a sufficient structural coverage of polytopic membrane proteins. CAMPS 2.0 is available at

  9. Increases in cAMP, MAPK activity, and CREB phosphorylation during REM sleep: implications for REM sleep and memory consolidation. (United States)

    Luo, Jie; Phan, Trongha X; Yang, Yimei; Garelick, Michael G; Storm, Daniel R


    The cyclic adenosine monophosphate (cAMP), mitogen-activated protein kinase (MAPK), and cAMP response element-binding protein (CREB) transcriptional pathway is required for consolidation of hippocampus-dependent memory. In mice, this pathway undergoes a circadian oscillation required for memory persistence that reaches a peak during the daytime. Because mice exhibit polyphasic sleep patterns during the day, this suggested the interesting possibility that cAMP, MAPK activity, and CREB phosphorylation may be elevated during sleep. Here, we report that cAMP, phospho-p44/42 MAPK, and phospho-CREB are higher in rapid eye movement (REM) sleep compared with awake mice but are not elevated in non-REM sleep. This peak of activity during REM sleep does not occur in mice lacking calmodulin-stimulated adenylyl cyclases, a mouse strain that learns but cannot consolidate hippocampus-dependent memory. We conclude that a preferential increase in cAMP, MAPK activity, and CREB phosphorylation during REM sleep may contribute to hippocampus-dependent memory consolidation.

  10. Nou equipament poliesportiu, piscines i urbanització (de l'obertura C/ Camp del ferro)


    Viladot Solé, Albert


    El projecte parteix del concurs proposat per BIMSA a la ubicació anomenada Camp del Ferro, al barri de la Sagrera, a Barcelona. Aquest concurs respon a la necessitat de construir un nou poliesportiu que substitueixi l'existent, situat a un solar a pocs metres de l'espai intervingut, i afectat pel futur parc lineal de la Sagrera. Aquest poliesportiu municipal té actualment l'hoquei patins com a activitat predominant i és la seu de l'Associació Esportiva Sant Andreu. A part de l'hoquei, també h...

  11. The molecular biology of memory: cAMP, PKA, CRE, CREB-1, CREB-2, and CPEB. (United States)

    Kandel, Eric R


    The analysis of the contributions to synaptic plasticity and memory of cAMP, PKA, CRE, CREB-1, CREB-2, and CPEB has recruited the efforts of many laboratories all over the world. These are six key steps in the molecular biological delineation of short-term memory and its conversion to long-term memory for both implicit (procedural) and explicit (declarative) memory. I here first trace the background for the clinical and behavioral studies of implicit memory that made a molecular biology of memory storage possible, and then detail the discovery and early history of these six molecular steps and their roles in explicit memory.

  12. The molecular biology of memory: cAMP, PKA, CRE, CREB-1, CREB-2, and CPEB

    Directory of Open Access Journals (Sweden)

    Kandel Eric R


    Full Text Available Abstract The analysis of the contributions to synaptic plasticity and memory of cAMP, PKA, CRE, CREB-1, CREB-2, and CPEB has recruited the efforts of many laboratories all over the world. These are six key steps in the molecular biological delineation of short-term memory and its conversion to long-term memory for both implicit (procedural and explicit (declarative memory. I here first trace the background for the clinical and behavioral studies of implicit memory that made a molecular biology of memory storage possible, and then detail the discovery and early history of these six molecular steps and their roles in explicit memory.

  13. Offering a Forensic Science Camp To Introduce and Engage High School Students in Interdisciplinary Science Topics

    DEFF Research Database (Denmark)

    Ahrenkiel, Linda; Worm-Leonhard, Martin


    In this article, we present details of a one-week interdisciplinary science camp for high school students in Denmark, “Criminal Camp”. We describe the use of forensic science and simulated crimes as a common foundation for teaching the theory and practice of concepts in chemistry, physics......’ attitudes toward science in general and the applications within forensic science in particular. From the free-response answers given in the survey, it was furthermore inferred that the participants enjoyed working with science in an interdisciplinary context, and that they gained both concrete knowledge...

  14. Access to safe water and personal hygiene practices in the Kulandia Refugee Camp (Jerusalem

    Directory of Open Access Journals (Sweden)

    Mohamad Issa


    Full Text Available Diarrheal illness, frequently associated with fecal-oral transmission, is one of the leading causes of death worldwide. It is commonly preventable through the implementation of safe water practices. This experiment concerns how to best implement safe water practices in a quasi-permanent refugee camp setting with limited ability for structural changes. Specifically, we explore how health promotion activities that help identify target groups for hygiene interventions can play a role in disease prevention. An anonymous survey was conducted at the United Nations Relief and Works Agency Health Clinic in the Kulandia refugee camp to assess the safe water and personal hygiene practices. Demographic and social characteristics, accessible water and personal hygiene characteristics, and gastrointestinal (GI burden for individuals and their households were assessed. A total of 96 individuals were enrolled; 62 females and 34 males. Approximately 58% of the sample had soap available and washed hands before and after eating and when preparing food. Piped water was the main source of drinking water (62%, while 31% of our sample utilized tanker-trucks. 93% of participants had access to toilet facilities, with 86% of these facilities being private households. 55% practice extra water hygiene measures on their household drinking water source. 51.3% considered vendor cleanliness when they were buying food. 51% had received formal health education. 68.8% had been taught by their parents, but only 55.2% were teaching their children and 15.6% had consistent access to a health professional for hygiene inquiries. Individual variables and hygiene practices associated with lower rates of diarrheal illnesses included having water piped into the home, proper hand washing, adequate soap availability, proper consideration of vendor cleanliness, higher income, levels of education, health hygiene education, and having access to healthcare professions to discuss hygiene related

  15. Access to Safe Water and Personal Hygiene Practices in the Kulandia Refugee Camp (Jerusalem). (United States)

    Issa, Mohamad; McHenry, Michael; Issa, Abdul Aziz; Blackwood, R Alexander


    Diarrheal illness, frequently associated with fecal-oral transmission, is one of the leading causes of death worldwide. It is commonly preventable through the implementation of safe water practices. This experiment concerns how to best implement safe water practices in a quasi-permanent refugee camp setting with limited ability for structural changes. Specifically, we explore how health promotion activities that help identify target groups for hygiene interventions can play a role in disease prevention. An anonymous survey was conducted at the United Nations Relief and Works Agency Health Clinic in the Kulandia refugee camp to assess the safe water and personal hygiene practices. Demographic and social characteristics, accessible water and personal hygiene characteristics, and gastrointestinal (GI) burden for individuals and their households were assessed. A total of 96 individuals were enrolled; 62 females and 34 males. Approximately 58% of the sample had soap available and washed hands before and after eating and when preparing food. Piped water was the main source of drinking water (62%), while 31% of our sample utilized tanker-trucks. 93% of participants had access to toilet facilities, with 86% of these facilities being private households. 55% practice extra water hygiene measures on their household drinking water source. 51.3% considered vendor cleanliness when they were buying food. 51% had received formal health education. 68.8% had been taught by their parents, but only 55.2% were teaching their children and 15.6% had consistent access to a health professional for hygiene inquiries. Individual variables and hygiene practices associated with lower rates of diarrheal illnesses included having water piped into the home, proper hand washing, adequate soap availability, proper consideration of vendor cleanliness, higher income, levels of education, health hygiene education, and having access to healthcare professions to discuss hygiene related matters. This is

  16. 'Plasma Camp': A Different Approach to Professional Development for Physics Teachers

    Energy Technology Data Exchange (ETDEWEB)

    Andrew Post-Zwicker and Nicholas R. Guilbert


    The Plasma Physics and Fusion Energy Institute ('Plasma Camp') was inaugurated in 1998 as a way to address two areas of concern in the professional development of high-school physics teachers: involving teachers in the theory and methods of a current area of research in physics and connecting the research experience back into the classroom. The Institute, run jointly by a scientist and a teacher, immersed high-school teachers from across the country in laboratory investigations and in pedagogical projects for two weeks at Princeton University's Plasma Physics Laboratory. The goals, structure, and initial outcomes of the Institute are discussed.

  17. The Observatory-Camping: a place of posible apprenticeship to the education and popularization of the Astronomy (United States)

    Navone, H. D.; Gastaud, C.; Pattini, N.; Aquilano, R.

    The development of Astronomy in the Educations area presents limitations as: poor equipment, trouble in managing space and time, difficulty to make experiences. The sky contaminations in the city, because of the luminosity, make the astronomical observation just impossible. But, the formal education has a wonderful media for the Astronomy: camping; this activity is very close to physical education. The proposal is the transformation of camping in "observatory-camping", to introduce astronomical concepts by the exploration of the sky. The methodology for this job is the observation of the sky by the eyes, first, and then making use of binoculars and a telescope, far from urban centres. Finally, this experience has place in the Planta de Campamentos N° 7502 (Máximo Paz, Santa Fe), which belongs to Ministerio de Educación de la Provincia de Santa Fe. FULL TEXT IN SPANISH

  18. Race in California's prison fire camps for men: prison politics, space, and the racialization of everyday life. (United States)

    Goodman, Philip


    The vast majority of social scientists agree that race is "socially constructed." Yet many scholars of punishment and prisons still treat race as static, self-evident categories. One result is that not enough is known about the production, meanings, and consequences of race as experienced by prisoners and those who guard and manage them. The author's research on California's prison fire camps uncovers the micro-level ways in which race is performed and imbued with meaning; he reveals how racial understandings color people and settings. One puzzle is that prisoners in California's fire camps will fight natural disasters side by side, sharing water and provisions, but separate into racial groups when in the camp itself. In part to answer this (and in part to develop better understandings of race and prisons more generally), the author unpacks the variegated nature of punishment and the spatialization of race and advocates for research that is faithful to the constructivist framework.

  19. Interpreting performance of mammograms by radiology residents trained in breast imaging: Comparison with radiologists who attend mammography boot camp

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Eun Hye [Dept. of Radiology, Bucheon Hospital, Soonchunhyang University College of Medicine, Bucheon (Korea, Republic of); Kim, Sung Hun [Dept. of Radiology, Seoul St. Mary' s Hospital, The Catholic University of Korea College of Medicine, Seoul (Korea, Republic of); An, Jin Kyung [Dept. of Radiology, Eulji General Hospital, Eulji University College of Medicine, Seoul (Korea, Republic of); Choi, Seon Hyeong [Dept. of Radiology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul (Korea, Republic of); Kim, Suk Jung [Dept. of Radiology, Haeundae Paik Hospital, Inje University College of Medicine, Busan (Korea, Republic of)


    To compare the interpreting performance of mammograms between residents trained in breast imaging and radiologists who attended the mammography boot camp (MBC) suggesting directions for training in breast imaging. We compared the performance of 61 residents trained in breast imaging for more than 2 months at 21 institutions with that of 141 radiologists who attended MBC using the same test case series. We compared mean scores and rates of correct answers between the two groups and examined residents' mean scores varied by institution. Residents' mean score was 60.9 ± 12.2 and radiologists' mean scores were 56.0 ± 12.2 (p = 0.004) and 78.3 ± 9.2 (p < 0.001) before and after camp, respectively. Residents were superior to pre-camp radiologists in interpreting microcalcifications (70.5% vs. 56.4%; p < 0.001) and true-negative cases (71.8% vs. 59.2%; p < 0.001). They were inferior to post-camp radiologists in interpreting mass/asymmetry (56.7% vs. 86.6%; p < 0.001) and microcalcifications (70.5% vs. 90.3%; p < 0.001). The mean score of all institutions except one was 61.8 ± 9.4. Trained residents' interpreting performance of mammograms is superior to that of pre-camp radiologists but inferior to that of post-camp radiologists. Substantial training in interpreting mammograms during residency is suggested.

  20. Characterization of a new CAMP factor carried by an integrative and conjugative element in Streptococcus agalactiae and spreading in Streptococci.

    Directory of Open Access Journals (Sweden)

    Sarah Chuzeville

    Full Text Available Genetic exchanges between Streptococci occur frequently and contribute to their genome diversification. Most of sequenced streptococcal genomes carry multiple mobile genetic elements including Integrative and Conjugative Elements (ICEs that play a major role in these horizontal gene transfers. In addition to genes involved in their mobility and regulation, ICEs also carry genes that can confer selective advantages to bacteria. Numerous elements have been described in S. agalactiae especially those integrated at the 3' end of a tRNA(Lys encoding gene. In strain 515 of S. agalactiae, an invasive neonate human pathogen, the ICE (called 515_tRNA(Lys is functional and carries different putative virulence genes including one encoding a putative new CAMP factor in addition to the one previously described. This work demonstrated the functionality of this CAMP factor (CAMP factor II in Lactococcus lactis but also in pathogenic strains of veterinary origin. The search for co-hemolytic factors in a collection of field strains revealed their presence in S. uberis, S. dysgalactiae, but also for the first time in S. equisimilis and S. bovis. Sequencing of these genes revealed the prevalence of a species-specific factor in S. uberis strains (Uberis factor and the presence of a CAMP factor II encoding gene in S. bovis and S. equisimilis. Furthermore, most of the CAMP factor II positive strains also carried an element integrated in the tRNA(Lys gene. This work thus describes a CAMP factor that is carried by a mobile genetic element and has spread to different streptococcal species.

  1. Opioid receptor activation triggering downregulation of cAMP improves effectiveness of anti-cancer drugs in treatment of glioblastoma (United States)

    Friesen, Claudia; Hormann, Inis; Roscher, Mareike; Fichtner, Iduna; Alt, Andreas; Hilger, Ralf; Debatin, Klaus-Michael; Miltner, Erich


    Glioblastoma are the most frequent and malignant human brain tumors, having a very poor prognosis. The enhanced radio- and chemoresistance of glioblastoma and the glioblastoma stem cells might be the main reason why conventional therapies fail. The second messenger cyclic AMP (cAMP) controls cell proliferation, differentiation, and apoptosis. Downregulation of cAMP sensitizes tumor cells for anti-cancer treatment. Opioid receptor agonists triggering opioid receptors can activate inhibitory Gi proteins, which, in turn, block adenylyl cyclase activity reducing cAMP. In this study, we show that downregulation of cAMP by opioid receptor activation improves the effectiveness of anti-cancer drugs in treatment of glioblastoma. The µ-opioid receptor agonist D,L-methadone sensitizes glioblastoma as well as the untreatable glioblastoma stem cells for doxorubicin-induced apoptosis and activation of apoptosis pathways by reversing deficient caspase activation and deficient downregulation of XIAP and Bcl-xL, playing critical roles in glioblastomas’ resistance. Blocking opioid receptors using the opioid receptor antagonist naloxone or increasing intracellular cAMP by 3-isobutyl-1-methylxanthine (IBMX) strongly reduced opioid receptor agonist-induced sensitization for doxorubicin. In addition, the opioid receptor agonist D,L-methadone increased doxorubicin uptake and decreased doxorubicin efflux, whereas doxorubicin increased opioid receptor expression in glioblastomas. Furthermore, opioid receptor activation using D,L-methadone inhibited tumor growth significantly in vivo. Our findings suggest that opioid receptor activation triggering downregulation of cAMP is a promising strategy to inhibit tumor growth and to improve the effectiveness of anti-cancer drugs in treatment of glioblastoma and in killing glioblastoma stem cells. PMID:24626197

  2. Systems biology investigation of cAMP modulation to increase SMN levels for the treatment of spinal muscular atrophy.

    Directory of Open Access Journals (Sweden)

    Sean G Mack

    Full Text Available Spinal muscular atrophy (SMA, a leading genetic cause of infant death worldwide, is an autosomal recessive disorder caused by the loss of SMN1 (survival motor neuron 1, which encodes the protein SMN. The loss of SMN1 causes a deficiency in SMN protein levels leading to motor neuron cell death in the anterior horn of the spinal cord. SMN2, however, can also produce some functional SMN to partially compensate for loss of SMN1 in SMA suggesting increasing transcription of SMN2 as a potential therapy to treat patients with SMA. A cAMP response element was identified on the SMN2 promoter, implicating cAMP activation as a step in the transcription of SMN2. Therefore, we investigated the effects of modulating the cAMP signaling cascade on SMN production in vitro and in silico. SMA patient fibroblasts were treated with the cAMP signaling modulators rolipram, salbutamol, dbcAMP, epinephrine and forskolin. All of the modulators tested were able to increase gem formation, a marker for SMN protein in the nucleus, in a dose-dependent manner. We then derived two possible mathematical models simulating the regulation of SMN2 expression by cAMP signaling. Both models fit well with our experimental data. In silico treatment of SMA fibroblasts simultaneously with two different cAMP modulators resulted in an additive increase in gem formation. This study shows how a systems biology approach can be used to develop potential therapeutic targets for treating SMA.

  3. Signaling transcript profile of the asexual intraerythrocytic development cycle of Plasmodium falciparum induced by melatonin and cAMP (United States)

    Rozanski, Andrei; Parreira, Kleber S.; Moraes, Miriam S.; Martins, David C.; Hashimoto, Ronaldo F.; Galante, Pedro A.F.; Garcia, Célia R.S.


    According to the World Health Organization (WHO), Plasmodium falciparum is the deadliest parasite among all species. This parasite possesses the ability to sense molecules, including melatonin (MEL) and cAMP, and modulate its cell cycle accordingly. MEL synchronizes the development of this malaria parasite by activating several cascades, including the generation of the second messenger cAMP. Therefore, we performed RNA sequencing (RNA-Seq) analysis in P. falciparum erythrocytic stages (ring, trophozoite and schizont) treated with MEL and cAMP. To investigate the expression profile of P. falciparum genes regulated by MEL and cAMP, we performed RNA-Seq analysis in three P. falciparum strains (control, 3D7; protein kinase 7 knockout, PfPK7-; and PfPK7 complement, PfPK7C). In the 3D7 strain, 38 genes were differentially expressed upon MEL treatment; however, none of the genes in the trophozoite (T) stage PfPK7- knockout parasites were differentially expressed upon MEL treatment for 5 hours compared to untreated controls, suggesting that PfPK7 may be involved in the signaling leading to differential gene expression. Moreover, we found that MEL modified the mRNA expression of genes encoding membrane proteins, zinc ion-binding proteins and nucleic acid-binding proteins, which might influence numerous functions in the parasite. The RNA-Seq data following treatment with cAMP show that this molecule modulates different genes throughout the intraerythrocytic cycle, namely, 75, 101 and 141 genes, respectively, in the ring (R), T and schizont (S) stages. Our results highlight P. falciparum's perception of the external milieu through the signaling molecules MEL and cAMP, which are able to drive to changes in gene expression in the parasite. PMID:28050233

  4. [Suitability of spatial pattern of camping sites in Langxiang Natural Reserve, Northeast Chi- na, based on GIS technology]. (United States)

    Yuan, Wei; Zhang, Jie; Tan Ji-qiang; Zhou, Bo; Kang, Rui-cun; Wang, Ai-hong; Liu, Wei; Zhang, Lu


    It is an effective way for natural reserves to enhance self-supportive ability and realize sustainable development by developing ecotourism. Taking the experimental zone of Langxiang Natural Reserve in Heilongjiang Province as research object, the forest sub-compartment as research unit, and spatial pattern of environmental suitability of camping sites as research content, an evaluation index system taking natural environment, geographical security, infrastructure and traffic as project levels was built. Delphi and AHP methods were used to determine index weights. A spatial distribution map of camping environmental suitability in Langxiang Natural Reserve was drawn using the GIS spatial information processing technology based on "3S" measurement and the survey data. The results showed that the highest score for quantification of environmental suitability was 90, while the lowest score was 78, and the average value was 83.66 in the 1067 forest sub-compartments for test. The area of forest sub-compartments which were suitable for camping was 1094.44 hm2, being 12.2% of the experimental zone. The forest sub-compartments which had high environmental suitability in the research area were distributed uniformly and centralized with low degree of fragmentation. It was suggested that the contiguous forest sub-compartments with high scores of environmental suitability could be integrated for camping tourism. Due to the high level of environmental suitability for camping, the experimental zone of Langxiang Natural Reserve is suitable for developing camping tourism. Based on "3S" technology, the land use conditions of ecotourism environment of a natural reserve could be evaluated quickly and quantitatively by mathematical model.

  5. [C-AMP concentration in various organs of female rats and in human ovaries with aging (author's transl)]. (United States)

    Arima, M


    The change in 3',5'-cyclic adenosine monophosphate (c-AMP) concentration was observed in various organs of rats in gonadal cycle in adult group and with aging (30, 70, 100, 120 weeks), and in human ovaries with aging. 1) The average c-AMP concentration of ovaries of rats showed a significant change with estrus cycle and was higher in the following sequence: proestrus, diestrus II, diestrus I and estrus phase. This tendency was also seen in hypothalamus and pituitary, but was not statistically significant, 2) The average c-AMP concentration in tissues began to decline significantly from 70 weeks in cerebral cortex and hypothalamus, and from 80 weeks in ovaries. However, on the other hand the concentrations in pituitary, liver and adrenal declined markedly from 100 weeks. 3) The c-AMP in ovaries of 80 weeks rats by pregnant mare serum (PMS) road increased by 0.5-fold in concentration, and by 0.6-fold in whole tissue relative to that of 30 weeks rats. 4) A significant difference in serum LH and FSH level between ovarian artery and vein was not found in cycling mature group, non-cycling climacteric group and post-menopausal group of women. 5) Both average concentrations and total values of c-AMP in ovaries of non-cycling climacteric and post-menopausal women were lower than those of mature cycling women. This fact may imply a different response by ovarian tissues such as corpus luteum, follicle and other tissues to gonadotropin. From these results of c-AMP in tissues, it is concluded that the decline of ovarian function with aging of rats was relatively earlier than pituitary, although being delayed compared with hypothalamus, and were the ovarian function in humans declined in the premenopausal period.

  6. War, oppression, refugee camps fuel spread of HIV. Migration and HIV. (United States)


    Evidence from countries such as Rwanda, Bosnia, and Sierra Leone links war and forced migration to the spread of HIV. In complex emergencies such as war, the social cohesion characteristic of stable societies is disrupted and families are dispersed, thereby increasing people's vulnerability. An estimated 30,000-40,000 women were raped during the war in Bosnia. In refugee camps, women may be forced to trade sex for food and protection for themselves and their children. Even when refugees are integrated into receiving communities, they remain vulnerable to sexual exploitation. Medical practitioners in refugee settings tend to emphasize diseases such as diarrhea, malaria, and respiratory illnesses. After a complex emergency, when a minimum range of health services is being re-established, HIV prevention is often considered a secondary issue. The International Federation of the Red Cross has advocated meeting the sexual health needs of refugees during the first 6 weeks of an emergency situation. The Federation provides condoms to refugees in transit and assistance to rape victims. The United Nations High Commission on Refugees ensures that refugee camps provide HIV/AIDS information, access to condoms, screening of donated blood, and observance of universal medical precautions.

  7. Plasma levels of cAMP, cGMP and CGRP in sildenafil-induced headache

    DEFF Research Database (Denmark)

    Kruuse, Christina Rostrup; Frandsen, E; Schifter, S;


    Sildenafil, a selective inhibitor of the cyclic guanosine monophosphate (cGMP) degrading phosphodiestrase 5 (PDE5), induced migraine without aura in 10 of 12 migraine patients and in healthy subjects it induced significantly more headache than placebo. The aim of the present study was to determin...... an important role of these signalling molecules, the present study questions whether cAMP and cGMP in peripheral blood can be used for monitoring pathophysiological events in headache and migraine mechanisms.......Sildenafil, a selective inhibitor of the cyclic guanosine monophosphate (cGMP) degrading phosphodiestrase 5 (PDE5), induced migraine without aura in 10 of 12 migraine patients and in healthy subjects it induced significantly more headache than placebo. The aim of the present study was to determine...... whether the pain-inducing effects of sildenafil would be reflected in plasma levels of important signalling molecules in migraine: cGMP, cyclic adenosine monophosphate (cAMP) and calcitonin gene-related peptide (CGRP). Ten healthy subjects (four women, six men) and 12 patients (12 women) suffering from...

  8. The RGS protein Crg2 regulates both pheromone and cAMP signalling in Cryptococcus neoformans. (United States)

    Xue, Chaoyang; Hsueh, Yen-Ping; Chen, Lydia; Heitman, Joseph


    G proteins orchestrate critical cellular functions by transducing extracellular signals into internal signals and controlling cellular responses to environmental cues. G proteins typically function as switches that are activated by G protein-coupled receptors (GPCRs) and negatively controlled by regulator of G protein signalling (RGS) proteins. In the human fungal pathogen Cryptococcus neoformans, three G protein alpha subunits (Gpa1, Gpa2 and Gpa3) have been identified. In a previous study, we identified the RGS protein Crg2 involved in regulating the pheromone response pathway through Gpa2 and Gpa3. In this study, a role for Crg2 was established in the Gpa1-cAMP signalling pathway that governs mating and virulence. We show that Crg2 physically interacts with Gpa1 and crg2 mutations increase cAMP production. crg2 mutations also enhance mating filament hyphae production, but reduce cell-cell fusion and sporulation efficiency during mating. Although crg2 mutations and the Gpa1 dominant active allele GPA1(Q284L) enhanced melanin production under normally repressive conditions, virulence was attenuated in a murine model. We conclude that Crg2 participates in controlling both Gpa1-cAMP-virulence and pheromone-mating signalling cascades and hypothesize it may serve as a molecular interface between these two central signalling conduits.

  9. Training Diaries during Altitude Training Camp in Two Olympic Champions: An Observational Case Study

    Directory of Open Access Journals (Sweden)

    Lorenzo Pugliese, Fabio R. Serpiello, Grégoire P. Millet, Antonio La Torre


    Full Text Available Traditionally, Live High-Train High (LHTH interventions were adopted when athletes trained and lived at altitude to try maximising the benefits offered by hypoxic exposure and improving sea level performance. Nevertheless, scientific research has proposed that the possible benefits of hypoxia would be offset by the inability to maintain high training intensity at altitude. However, elite athletes have been rarely recruited as an experimental sample, and training intensity has almost never been monitored during altitude research. This case study is an attempt to provide a practical example of successful LHTH interventions in two Olympic gold medal athletes. Training diaries were collected and total training volumes, volumes at different intensities, and sea level performance recorded before, during and after a 3-week LHTH camp. Both athletes successfully completed the LHTH camp (2090 m maintaining similar absolute training intensity and training volume at high-intensity (> 91% of race pace compared to sea level. After the LHTH intervention both athletes obtained enhancements in performance and they won an Olympic gold medal. In our opinion, LHTH interventions can be used as a simple, yet effective, method to maintain absolute, and improve relative training intensity in elite endurance athletes.

  10. Funktionelle in vitro-Effekte CAMP/CGMP-modulierender Pharmaka am humanen Detrusormuskel

    Directory of Open Access Journals (Sweden)

    Ückert St


    Full Text Available Die zyklischen Nukleotidmonophosphate cAMP und cGMP regulieren als intrazelluläre Second Messenger zahlreiche Gewebe- und Organfunktionen. cAMP und cGMP werden von zellulären Adenylat- und Guanylatzyklasen synthetisiert und von Phosphodiesterasen degradiert, die somit Schlüsselenzyme im Prozeß der Tonusregulation glatter Muskulatur sind. Die Markteinführung des PDE5-Inhibitors Sildenafil (Viagra hat dem Konzept der PDE-Inhibition auch in der Urologie breite Akzeptanz verschafft. Eigene Arbeiten der vergangenen Jahre beschreiben die Präsenz der PDE-Isoenzyme 1, 2, 3, 4 und 5 in der Muskulatur des humanen Detrusors und zeigen das klinische Potential des PDE1-Inhibitors Vinpocetin in der Behandlung der motorischen Dranginkontinenz. Mit dem Ziel der Charakterisierung geeigneter Substanzen für die Pharmakotherapie der Detrusorhyperaktivität haben wir die in vitro-Effekte neuer, selektiver Inhibitoren der PDE des Typs 2, 3 und 5 auf isolierte humane Detrusormuskulatur untersucht und mit denen des Diterpens Forskolin (Aktivator der Adenylatzyklase und der Stickoxid (NO-Donatoren Dihydropyridin (DHP und Na+Nitroprussid (NNP verglichen.

  11. cAMP receptor protein (CRP) downregulates Klebsiella pneumoniae nif promoters in Escherichia coli

    Institute of Scientific and Technical Information of China (English)


    In enteric bacteria, in response to the PTS system, the cAMP receptor protein (CRP) mediates the glucose effect, via regulating s70-dependent catabolic genes at transcriptional level. In this study, it is observed that the nitrogen fixation capacity of Klebsiella pneumoniae varies strongly when cells are grown on different carbohydrates, and this carbon effect occurs at the level of nif gene expression. Here we show that CRP can repress s54-dependent nif promoters (nifB, nifE, nifF, nifH, nifJ, nifLA and nifU), in a cAMP dependent fashion, in closed related E. coli background. Sequence analysis of these nif promoters indicates that there is no direct correlation between the fold of CRP-cAMP-mediated inhibition and the upstream cis elements at the promoters. In addition, the crp gene of K. pneumoniae has been isolated and sequenced, which is structural and functional highly homologous to that of E. coli. This suggests that CRP-cAMP-mediated inhibition on the nif promoters could be the reason for carbon effect on nitrogen fixation and thus has its physiological significance. A novel regulatory linkage between carbon metabolism and nitrogen fixation is proposed.

  12. The NDUFS4 nuclear gene of complex I of mitochondria and the cAMP cascade. (United States)

    Papa, Sergio


    Results of studies on the role of the 18 kDa (IP) polypeptide subunit of complex I, encoded by the nuclear NDUFS4 gene, in isolated bovine heart mitochondria and human and murine cell cultures are presented.The mammalian 18 kDa subunit has in the carboxy-terminal sequence a conserved consensus site (RVS), which in isolated mitochondria is phosphorylated by cAMP-dependent protein kinase (PKA). The catalytic and regulatory subunits of PKA have been directly immunodetected in the inner membrane/matrix fraction of mammalian mitochondria. In the mitochondrial inner membrane a PP2Cgamma-type phosphatase has also been immunodetected, which dephosphorylates the 18 kDa subunit, phosphorylated by PKA. This phosphatase is Mg(2+)-dependent and inhibited by Ca(2+). In human and murine fibroblast and myoblast cultures "in vivo", elevation of intracellular cAMP level promotes phosphorylation of the 18 kDa subunit and stimulates the activity of complex I and NAD-linked mitochondrial respiration. Four families have been found with different mutations in the cDNA of the NDUFS4 gene. These mutations, transmitted by autosomal recessive inheritance, were associated in homozygous children with fatal neurological syndrome. All these mutations destroyed the phosphorylation consensus site in the C terminus of the 18 kDa subunit, abolished cAMP activation of complex I and impaired its normal assembly.

  13. Excerpt from After Camp: Portraits in Midcentury Japanese American Life and Politics

    Directory of Open Access Journals (Sweden)

    Greg Robinson


    Full Text Available In his book By Order of the President: FDR and the Internment of Japanese Americans (2001, Greg Robinson discussed FDR’s decision to remove Japanese Americans from their homes and concentrate them in internment camps. Now in this chapter from his recently published book, After Camp: Portraits in Midcentury Japanese American Life and Politics, he unearths Roosevelt’s grandiose and frightening idea of their return to civil society by scattering them—two or three families at a time—in small towns, all away from the west coast. He also thought such a scatter plan would suit refugee European Jews who he hoped would settle in Latin America. Indeed he thought ethnic minorities crowded into American cities might also benefit if resettled in small towns. While this is a racial story, FDR’s vision here was also driven by the rural sentiments of FDR, the gentleman farmer. That produced some highly regarded programs, most notably the Civilian Conservation Corps (CCC and the “Greenbelt towns.” But the massive population transfers that Robinson shows to have been on the president’s mind would have made a mockery of the US rights tradition. Of equal importance, Robinson’s examination of the role of major social scientists recruited to the project provides an object lesson in the dangers of intellect seduced by power.

  14. Exchange factors directly activated by cAMP mediate melanocortin 4 receptor-induced gene expression (United States)

    Glas, Evi; Mückter, Harald; Gudermann, Thomas; Breit, Andreas


    Gs protein-coupled receptors regulate many vital body functions by activation of cAMP response elements (CRE) via cAMP-dependent kinase A (PKA)-mediated phosphorylation of the CRE binding protein (CREB). Melanocortin 4 receptors (MC4R) are prototypical Gs-coupled receptors that orchestrate the hypothalamic control of food-intake and metabolism. Remarkably, the significance of PKA for MC4R-induced CRE-dependent transcription in hypothalamic cells has not been rigorously interrogated yet. In two hypothalamic cell lines, we observed that blocking PKA activity had only weak or no effects on reporter gene expression. In contrast, inhibitors of exchange factors directly activated by cAMP-1/2 (EPAC-1/2) mitigated MC4R-induced CRE reporter activation and mRNA induction of the CREB-dependent genes c-fos and thyrotropin-releasing hormone. Furthermore, we provide first evidence that extracellular-regulated kinases-1/2 (ERK-1/2) activated by EPACs and not PKA are the elusive CREB kinases responsible for MC4R-induced CREB/CRE activation in hypothalamic cells. Overall, these data emphasize the pivotal role of EPACs rather than PKA in hypothalamic gene expression elicited by a prototypical Gs-coupled receptor. PMID:27612207

  15. Cross-talk between signaling pathways can generate robust oscillations in calcium and cAMP.

    Directory of Open Access Journals (Sweden)

    Fernando Siso-Nadal

    Full Text Available BACKGROUND: To control and manipulate cellular signaling, we need to understand cellular strategies for information transfer, integration, and decision-making. A key feature of signal transduction is the generation of only a few intracellular messengers by many extracellular stimuli. METHODOLOGY/PRINCIPAL FINDINGS: Here we model molecular cross-talk between two classic second messengers, cyclic AMP (cAMP and calcium, and show that the dynamical complexity of the response of both messengers increases substantially through their interaction. In our model of a non-excitable cell, both cAMP and calcium concentrations can oscillate. If mutually inhibitory, cross-talk between the two second messengers can increase the range of agonist concentrations for which oscillations occur. If mutually activating, cross-talk decreases the oscillation range, but can generate 'bursting' oscillations of calcium and may enable better filtering of noise. CONCLUSION: We postulate that this increased dynamical complexity allows the cell to encode more information, particularly if both second messengers encode signals. In their native environments, it is unlikely that cells are exposed to one stimulus at a time, and cross-talk may help generate sufficiently complex responses to allow the cell to discriminate between different combinations and concentrations of extracellular agonists.

  16. Antidiabetic sulfonylureas and cAMP cooperatively activate Epac2A. (United States)

    Takahashi, Toshimasa; Shibasaki, Tadao; Takahashi, Harumi; Sugawara, Kenji; Ono, Aika; Inoue, Naoko; Furuya, Toshio; Seino, Susumu


    Sulfonylureas are widely used drugs for treating insulin deficiency in patients with type 2 diabetes. Sulfonylureas bind to the regulatory subunit of the pancreatic β cell potassium channel that controls insulin secretion. Sulfonylureas also bind to and activate Epac2A, a member of the Epac family of cyclic adenosine monophosphate (cAMP)-binding proteins that promote insulin secretion through activation of the Ras-like guanosine triphosphatase Rap1. Using molecular docking simulation, we identified amino acid residues in one of two cyclic nucleotide-binding domains, cNBD-A, in Epac2A predicted to mediate the interaction with sulfonylureas. We confirmed the importance of the identified residues by site-directed mutagenesis and analysis of the response of the mutants to sulfonylureas using two assays: changes in fluorescence resonance energy transfer (FRET) of an Epac2A-FRET biosensor and direct sulfonylurea-binding experiments. These residues were also required for the sulfonylurea-dependent Rap1 activation by Epac2A. Binding of sulfonylureas to Epac2A depended on the concentration of cAMP and the structures of the drugs. Sulfonylureas and cAMP cooperatively activated Epac2A through binding to cNBD-A and cNBD-B, respectively. Our data suggest that sulfonylureas stabilize Epac2A in its open, active state and provide insight for the development of drugs that target Epac2A.

  17. Quality of life of Syrian refugees living in camps in the Kurdistan Region of Iraq. (United States)

    Aziz, Izaddin A; Hutchinson, Claire V; Maltby, John


    The current study explores the perceived quality of life of Syrian refugees who have entered the Kurdistan Region of Iraq. Two hundred and seventy participants residing in refugee camps in the Erbil region in Kurdistan completed the WHOQOL-BREF, which measures Quality of Life (QOL) within four domains; physical, psychological, social relationships and environment. Syrian refugees in Kurdistan scored significantly lower for general population norms on physical health, psychological and environment QOL, and score significantly lower for physical health and psychological QOL for refugees in the Gaza strip. However, respondents in the current sample scored significantly higher on environment QOL compared to refugees in the Gaza strip, and significantly higher on all the QOL domains than those reported for refugees in West Africa. Finally, Syrian refugees in Kurdistan scored significantly higher than general population norms for social relationships QOL. The current findings provide the first report of QOL domain scores among Syrian refugees in the Kurdistan camps and suggest that social relationships and environmental QOL circumstances are relatively satisfactory, and that further investigation might be focused on physical and psychological QOL.

  18. cAMP Stimulation of HCO3- Secretion Across Airway Epithelia

    Directory of Open Access Journals (Sweden)

    Welsh MJ


    Full Text Available To test for the presence of HCO(3(- transport across airway epithelia, we measured short-circuit current in primary cultures of canine and human airway epithelia bathed in a Cl(--free, HCO(3(-/CO(2-buffered solution. cAMP agonists stimulated a secretory current that was likely carried by HCO(3(- because it was absent in HCO(3(--free solutions. In addition, the cAMP-stimulated current was inhibited by the carbonic anhydrase inhibitor, acetazolamide, and by the apical addition of a blocker of cystic fibrosis transmembrane conductance regulator (CFTR, diphenylamine-2-carboxylate. The current was dependent on Na(+ because it was inhibited by removing Na(+ from the submucosal solution and by inhibition of the Na(+-K(+-ATPase with ouabain. The cAMP-stimulated current was absent in cystic fibrosis (CF airway epithelia. These data suggest that cAMP agonists can stimulate HCO(3(- secretion across airway epithelia and that CFTR may provide a conductive pathway for HCO(3(- movement across the apical membrane.

  19. Malaria in Kakuma refugee camp, Turkana, Kenya: facilitation of Anopheles arabiensis vector populations by installed water distribution and catchment systems

    Directory of Open Access Journals (Sweden)

    Cetron Martin S


    Full Text Available Abstract Background Malaria is a major health concern for displaced persons occupying refugee camps in sub-Saharan Africa, yet there is little information on the incidence of infection and nature of transmission in these settings. Kakuma Refugee Camp, located in a dry area of north-western Kenya, has hosted ca. 60,000 to 90,000 refugees since 1992, primarily from Sudan and Somalia. The purpose of this study was to investigate malaria prevalence and attack rate and sources of Anopheles vectors in Kakuma refugee camp, in 2005-2006, after a malaria epidemic was observed by staff at camp clinics. Methods Malaria prevalence and attack rate was estimated from cases of fever presenting to camp clinics and the hospital in August 2005, using rapid diagnostic tests and microscopy of blood smears. Larval habitats of vectors were sampled and mapped. Houses were sampled for adult vectors using the pyrethrum knockdown spray method, and mapped. Vectors were identified to species level and their infection with Plasmodium falciparum determined. Results Prevalence of febrile illness with P. falciparum was highest among the 5 to 17 year olds (62.4% while malaria attack rate was highest among the two to 4 year olds (5.2/1,000/day. Infected individuals were spatially concentrated in three of the 11 residential zones of the camp. The indoor densities of Anopheles arabiensis, the sole malaria vector, were similar during the wet and dry seasons, but were distributed in an aggregated fashion and predominantly in the same zones where malaria attack rates were high. Larval habitats and larval populations were also concentrated in these zones. Larval habitats were man-made pits of water associated with tap-stands installed as the water delivery system to residents with year round availability in the camp. Three percent of A. arabiensis adult females were infected with P. falciparum sporozoites in the rainy season. Conclusions Malaria in Kakuma refugee camp was due mainly

  20. [Quantitative and qualitative evaluation of group nourishment in 16-18 aged youths in a summer sports camp]. (United States)

    Gacek, Maria; Marian, Fiedor


    The purpose of this work was an attempt to evaluate the way of group nourishment among the teenage youths on a summer sports camp with respect to quantity and quality of food. The distribution of BMI rate among 165 girls and 131 boys--the participants of the camp was also estimated. The research showed that the analyzed decade menus were typically correct considering the average energetic value, they contained the overstandard amounts of protein and phosphorus, insufficiency of calcium, iron, magnesium and group B and C vitamins as well. At qualitative evaluation the analyzed menus received 21 points according to Starzynska's scale (satisfactory grade).

  1. cAMP increases surface expression of NKCC2 in rat thick ascending limbs: role of VAMP. (United States)

    Ortiz, Pablo A


    NaCl absorption by the thick ascending limb of Henle's loop (TAL) is mediated by the apical Na-K-2Cl cotransporter NKCC2. cAMP increases NaCl absorption in the TAL by stimulating NKCC2. In oocytes, cAMP increases NKCC2 activity by regulating its trafficking. However, the mechanism by which cAMP stimulates NKCC2 in TALs is not clear. We hypothesized that cAMP increases surface expression of NKCC2 and NaCl absorption in TALs and that vesicle-associated membrane protein (VAMP) is involved in this mechanism. We used surface biotinylation of rat medullary TALs (mTAL) to examine surface and total NKCC2 levels. When mTAL suspensions were treated with dibutyryl cAMP (db-cAMP) or forskolin plus IBMX for 20 min, surface NKCC2 expression increased by 126 +/- 23 and 92 +/- 17% above basal, respectively (P < 0.03). No changes in total NKCC2 expression were observed, suggesting that cAMP increased translocation of NKCC2. We studied the role of VAMP in NKCC2 translocation and found that incubating mTALs with tetanus toxin (30 nM), which inhibits vesicle trafficking by inactivating VAMP-2 and -3, completely blocked the stimulatory effect of db-cAMP on surface NKCC2 expression (tetanus toxin = 100% vs. tetanus toxin + db-cAMP = 102 +/- 21% of control; not significant). We studied VAMP-2 and -3 expression and localization in isolated perfused TALs by confocal microscopy and found that both of them were located in the subapical space of the TAL. Finally, in isolated perfused mTALs, db-cAMP increased net Cl absorption by 95.0 +/- 34.8% (P < 0.03), and pretreatment of TALs with tetanus toxin blocked the stimulation of Cl absorption (from 110.9 +/- 15.9 to 109.7 +/- 15.6 pmol.min(-1).mm(-1); not significant). We concluded that cAMP increases NKCC2 surface expression by a mechanism involving VAMP and that NKCC2 trafficking to the apical membrane is involved in the stimulation of TAL NaCl absorption by cAMP.

  2. Differences in HIV-related behaviors at Lugufu refugee camp and surrounding host villages, Tanzania

    Directory of Open Access Journals (Sweden)

    Mbaruku Godfrey


    Full Text Available Abstract Background An HIV behavioral surveillance survey was undertaken in November 2005 at Lugufu refugee camp and surrounding host villages, located near western Tanzania's border with the Democratic Republic of Congo (DRC. Methods The sample size was 1,743 persons based on cluster survey methodology. All members of selected households between 15–49 years old were eligible respondents. Questions included HIV-related behaviors, population displacement, mobility, networking and forced sex. Data was analyzed using Stata to measure differences in proportions (chi-square and differences in means (t-test between gender, age groups, and settlement location for variables of interest. Results Study results reflect the complexity of factors that may promote or inhibit HIV transmission in conflict-affected and displaced populations. Within this setting, factors that may increase the risk of HIV infections among refugees compared to the population in surrounding villages include young age of sexual initiation among males (15.9 years vs. 19.8 years, p = .000, high-risk sex partners in the 15–24 year age group (40% vs. 21%, χ2 33.83, p = .000, limited access to income (16% vs. 51% χ2 222.94, p = .000, and the vulnerability of refugee women, especially widowed, divorced and never-married women, to transactional sex (married vs. never married, divorced, widowed: for 15–24 age group, 4% and 18% respectively, χ2 8.07, p = .004; for 25–49 age group, 4% and 23% respectively, χ2 21.46, p = .000. A majority of both refugee and host village respondents who experienced forced sex in the past 12 months identified their partner as perpetrator (64% camp and 87% in villages. Although restrictions on movements in and out of the camp exist, there was regular interaction between communities. Condom use was found to be below 50%, and expanded population networks may also increase opportunities for HIV transmission. Availability of refugee health services may be

  3. cAMP dependent and independent regulation of thyroglobulin synthesis by two clones of the OVNIS 6H thyroid cell line. (United States)

    Aouani, A; Hovsépian, S; Fayet, G


    The hormonal regulation of thyroglobulin synthesis has been studied using two independent clones of the OVNIS 6H cell line. Insulin, hydrocortisone and TSH were able to stimulate thyroglobulin synthesis, whereas transferrin, somatostatin and glycyl-histidyl-lysine were without effect. Insulin stimulated thyroglobulin synthesis without affecting cAMP production. Hydrocortisone, when combined with insulin was a stimulator too; this stimulation was not accompanied by an increase in cAMP. TSH alone was unable to stimulate either cAMP or thyroglobulin synthesis. The stimulatory effect of TSH on thyroglobulin synthesis took place only when combined with insulin or insulin plus hydrocortisone, and was mediated by cAMP. Consequently, insulin and hydrocortisone stimulated thyroglobulin synthesis by cAMP-independent mechanisms, whereas TSH acted via the cAMP system. Forskolin mimicked TSH effects on cAMP and thyroglobulin synthesis. Calf serum inhibited cAMP and thyroglobulin production. Optimal cAMP and thyroglobulin synthesis as well as TSH responsiveness were obtained in serum-free medium supplemented with 5 micrograms/ml insulin, 100 nM hydrocortisone and 1 mU/ml TSH.

  4. An Infection Control Program for a 2009 Influenza A H1N1 Outbreak in a University-Based Summer Camp (United States)

    Tsalik, Ephraim L.; Cunningham, Coleen K.; Cunningham, Hannah M.; Lopez-Marti, Maria G.; Sangvai, Devdutta G.; Purdy, William K.; Anderson, Deverick J.; Thompson, Jessica R.; Brown, Monte; Woods, Christopher W.; Jaggers, L. Brett; Hendershot, Edward F.


    Objectives: Describe two 2009-H1N1 influenza outbreaks in university-based summer camps and the implementation of an infection control program. Participants: 7,906 campers across 73 residential camps from May 21-August 2, 2009. Methods: Influenza-like-illness (ILI) was defined as fever with cough and/or sore throat. Influenza A was identified…

  5. Transiently increasing cAMP levels selectively in hippocampal excitatory neurons during sleep deprivation prevents memory deficits caused by sleep loss

    NARCIS (Netherlands)

    Havekes, Robbert; Bruinenberg, Vibeke M.; Tudor, Jennifer C.; Ferri, Sarah L.; Baumann, Arnd; Meerlo, Peter; Abel, Ted


    The hippocampus is particularly sensitive to sleep loss. Although previous work has indicated that sleep deprivation impairs hippocampal cAMP signaling, it remains to be determined whether the cognitive deficits associated with sleep deprivation are caused by attenuated cAMP signaling in the hippoca

  6. Signal Transduction in Dictyostelium fgd A Mutants with a Defective Interaction between Surface cAMP Receptors and a GTP-binding Regulatory Protein

    NARCIS (Netherlands)

    Kesbeke, Fanja; Snaar-Jagalska, B. Ewa; Haastert, Peter J.M. van


    Transmembrane signal transduction was investigated in four Dictyostelium discoideum mutants that belong to the fgd A complementation group. The results show the following. (a) Cell surface cAMP receptors are present in fgd A mutants, but cAMP does not induce any of the intracellular responses, inclu

  7. 78 FR 40958 - Amendment of Restricted Areas R-2504A & R-2504B; Camp Roberts, CA, and Restricted Area R-2530... (United States)


    ...-2504B; Camp Roberts, CA, and Restricted Area R-2530; Sierra Army Depot, CA AGENCY: Federal Aviation...- 2504A and R-2504B, Camp Roberts, CA, and restricted area R-2530, Sierra Army Depot, CA, by removing the... time is in effect. The intended time of designation for restricted areas R-2504A and R- 2504B is...

  8. Dual role of cAMP and involvement of both G-proteins and ras in regulation of ERK2 in Dictyostelium discoideum. (United States)

    Knetsch, M L; Epskamp, S J; Schenk, P W; Wang, Y; Segall, J E; Snaar-Jagalska, B E


    Dictyostelium discoideum expresses two Extracellular signal Regulated Kinases, ERK1 and ERK2, which are involved in growth, multicellular development and regulation of adenylyl cyclase. Binding of extracellular cAMP to cAMP receptor 1, a G-protein coupled cell surface receptor, transiently stimulates phosphorylation, activation and nuclear translocation of ERK2. Activation of ERK2 by cAMP is dependent on heterotrimeric G-proteins, since activation of ERK2 is absent in cells lacking the Galpha4 subunit. The small G-protein rasD also activates ERK2. In cells overexpressing a mutated, constitutively active rasD, ERK2 activity is elevated prior to cAMP stimulation. Intracellular cAMP and cAMP-dependent protein kinase (PKA) are essential for adaptation of the ERK2 response. This report shows that multiple signalling pathways are involved in regulation of ERK2 activity in D.discoideum.

  9. Melanocortin 1 receptor mutations impact differentially on signalling to the cAMP and the ERK mitogen-activated protein kinase pathways. (United States)

    Herraiz, Cecilia; Jiménez-Cervantes, Celia; Zanna, Paola; García-Borrón, José C


    Melanocortin 1 receptor (MC1R), a Gs protein-coupled receptor expressed in melanocytes, is a major determinant of skin pigmentation, phototype and cancer risk. MC1R activates cAMP and mitogen-activated protein kinase ERK1/ERK2 signalling. When expressed in rat pheochromocytoma cell line cells, the R151C, R160W and D294H MC1R variants associated with melanoma and impaired cAMP signalling mediated ERK activation and ERK-dependent, agonist-induced neurite outgrowth comparable with wild-type. Dose-response curves for ERK activation and cAMP production indicated higher sensitivity of the ERK response. Thus, the melanoma-associated MC1R mutations impact differently on cAMP and ERK signalling, suggesting that cAMP is not responsible for functional coupling of MC1R to the ERK cascade.

  10. Signal transduction in Dictyostelium fgd A mutants with a defective interaction between surface cAMP receptors and a GTP-binding regulatory protein [published erratum appears in J Cell Biol 1988 Dec;107(6 Pt 1):following 2463



    Transmembrane signal transduction was investigated in four Dictyostelium discoideum mutants that belong to the fgd A complementation group. The results show the following. (a) Cell surface cAMP receptors are present in fgd A mutants, but cAMP does not induce any of the intracellular responses, including the activation of adenylate or guanylate cyclase and chemotaxis. (b) cAMP induces down- regulation and the covalent modification (presumably phosphorylation) of the cAMP receptor. (c) The inhi...

  11. Field camp: Using traditional methods to train the next generation of petroleum geologists (United States)

    Puckette, J.O.; Suneson, N.H.


    The summer field camp experience provides many students with their best opportunity to learn the scientific process by making observations and collecting, recording, evaluating, and interpreting geologic data. Field school projects enhance student professional development by requiring cooperation and interpersonal interaction, report writing to communicate interpretations, and the development of project management skills to achieve a common goal. The field school setting provides students with the opportunity to observe geologic features and their spatial distribution, size, and shape that will impact the student's future careers as geoscientists. The Les Huston Geology Field Camp (a.k.a. Oklahoma Geology Camp) near Ca??on City, Colorado, focuses on time-tested traditional methods of geological mapping and fieldwork to accomplish these goals. The curriculum consists of an introduction to field techniques (pacing, orienteering, measuring strike and dip, and using a Jacob's staff), sketching outcrops, section measuring (one illustrating facies changes), three mapping exercises (of increasing complexity), and a field geophysics project. Accurate rock and contact descriptions are emphasized, and attitudes and contacts are mapped in the field. Mapping is done on topographic maps at 1:12,000 and 1:6000 scales; air photos are provided. Global positioning system (GPS)-assisted mapping is allowed, but we insist that locations be recorded in the field and confirmed using visual observations. The course includes field trips to the Cripple Creek and Leadville mining districts, Floris-sant/Guffey volcano area, Pikes Peak batholith, and the Denver Basin. Each field trip is designed to emphasize aspects of geology that are not stressed in the field exercises. Students are strongly encouraged to accurately describe geologic features and gather evidence to support their interpretations of the geologic history. Concise reports are a part of each major exercise. Students are grouped

  12. Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi

    Directory of Open Access Journals (Sweden)

    Dilvani O. Santos


    Full Text Available Macromolecule synthesis of Trypanosoma cruzi in culture was monitored using radioactive tracers. Cells of different days in culture displayed a preferential incorporation of precursors as follows: 1 day for (³H-thymidine cells; 3 days for (³H-uridine cells, and 4 days for (³H-leucine cells. Autoradiographic studies showed that (³H-thymidine was incorporated in the DNA of both kinetoplast and nucleus in this order. Shifts in the intracellular content of cAMP either by addition of dibutyryl-cAMP or by stimulation of the adenylcyclase by isoproterenol, caused an inhibition in the synthesis of DNA, RNA and proteins. Addition to the T. cruzi cultures of these agents which elevate the intracellular content ofcAMP provoked an interruption of cell proliferation as a result of the impairment of macromolecule synthesis. A discrimination was observed among the stereoisomers of isoproterenol, the L configuration showing to be most active.A síntese de macromoléculas de T. cruzi em cultura foi monitorada usando traçadores radioativos. Células de diferentes dias em cultura mostraram uma incorporação preferencial de precursores comco se seguez: 1 dia para (3H-timidina; 3 dias para (3H-uridina e 4 dias para (3H-leucina. Estudos autoradiográficos mostraram que (3H-leucina. Estudos autoradiográficos mostraram que (3H-timidina foi incorporada no DNA de ambos, cinetoplasto e núcleo, nesta ordem. Alterações no conteúdo intracelular de cAMP seja por adição de dibutiril-cAMP ou por estimulação de adenilciclase por isoproterenol, causav am inibição na síntese de DNA, RNA e proteínas. A adição destes agentes que elevam o conteúdo intracelular de cAMP em culturas de T.cruzi provocou inibição de crescimento, com resultado da síntese macromolecular imperfeita. Foi observada uma discriminação entre os estereoisômeros de isoproterenol, sendo a configuração L, a mais ativa.

  13. Intimate partner physical violence among women in Shimelba refugee camp, northern Ethiopia

    Directory of Open Access Journals (Sweden)

    Feseha Girmatsion


    Full Text Available Abstract Background Domestic violence has unwanted effects on the physical and psychological well-being of women, which have been recognized globally as an important public health problem. Violence perpetrated by intimate partner is one form of domestic violence, a serious human rights abuse and a public health issue, among refugees owing to its substantial consequences for women's physical, mental and reproductive health problems. Because the incidents are under-reported, the true scale of the problem is unknown and unexamined among refugee women in Ethiopia. Thus, this study aim to assess the magnitude of intimate partner physical violence and associated factors among women in Shimelba refugee camp, Northern Ethiopia. Methods A community-based cross-sectional study was conducted among a sample of 422 refugee women from March to April 2011. A simple random sampling method was used to select the study subjects from seven zones of the refugee camp. Census was done to identify all households with women having an intimate partner. A pre-tested interviewer guided structured questionnaire was used for data collection. Data were entered, cleaned and analyzed using SPSS software version 16.0. Descriptive, bivariate and multivariate logistic regression analyses were done where applicable. A p-value less than 0.05 with 95% CI were set and used as a cut-off point to examine the statistical association between the explanatory and outcome variables. Results The prevalence of physical violence in the last 12 months and lifetime were 107(25.5% and 131(31.0% respectively. The commonest forms of physical violence reported included slapping 101(61.6% and throwing objects 32(19.5%. Significant risk factors associated with experiencing physical violence were being a farmer (AOR = 3.0[95%CI: 1.7, 5.5], knowing women in neighborhood whose husband to beat them (AOR = 1.87[95%CI: 1.0, 3.5], being a Muslim (AOR = 2.4 [95%C.I: 1.107, 5.5], and having a drunkard partner

  14. Summary of Fresher's Camp in the Department of Products Engineering and Environmental Management(Educationai Development Symposiums)


    鈴木,宏典; Hironori, Suzuki


    This article summarizes the fresher's camp in the department of PEEM (Products Engineering and Environmental Management), Nippon Institute of Technology. Not only the effect on newcomer student s, but also the benefit to the upperclass students were summarized in terms of the Project Based Learning. Further improvements were finally discussed.

  15. Santa Fe Indian Camp, House 21, Richmond, California: Persistence of Identity among Laguna Pueblo Railroad Laborers, 1945-1982. (United States)

    Peters, Kurt


    In 1880 the Laguna people and the predecessor of the Atchison, Topeka, and Santa Fe Railroad reached an agreement giving the railroad unhindered right-of-way through Laguna lands in exchange for Laguna employment "forever." Discusses the Laguna-railroad relationship through 1982, Laguna labor camps in California, and the persistence of…

  16. Addressing “Nature-Deficit Disorder”: A Mixed Methods Pilot Study of Young Adults Attending a Wilderness Camp

    Directory of Open Access Journals (Sweden)

    Sara L. Warber


    Full Text Available Background and Objectives. Rapid urbanization raises concern about chronic human health issues along with less frequent interaction with the natural world. “Nature-deficit disorder,” a nonclinical term, describes this potential impact on the well-being of youth. We conducted a mixed methods pilot study of young adults attending a four-week wilderness camp to investigate whether nature-based camp experiences would increase connection to nature and promote multiple dimensions of well-being. Methods. Participants completed precamp (n = 46 and postcamp (n = 36 online questionnaires including nature-related and holistic well-being measures. Differences were investigated using paired t-tests. Interviews (n = 16 explored camp experiences and social relations. Results. All nature-related measures—exposure, knowledge, skills, willingness to lead, perceived safety, sense of place, and nature connection—significantly increased. Well-being outcomes also significantly improved, including perceived stress, relaxation, positive and negative emotions, sense of wholeness, and transcendence. Physical activity and psychological measures showed no change. Interviews described how the wilderness environment facilitated social connections. Conclusion. Findings illustrate the change in nature relations and well-being that wilderness camp experiences can provide. Results can guide future research agendas and suggest that nature immersion experiences could address the risk of “nature-deficit disorder,” improve health, and prepare future environmental leaders.

  17. cAMP levels in fast- and slow-twitch skeletal muscle after an acute bout of aerobic exercise (United States)

    Sheldon, A.; Booth, F. W.; Kirby, C. R.


    The present study examined whether exercise duration was associated with elevated and/or sustained elevations of postexercise adenosine 3',5'-cyclic monophosphate (cAMP) by measuring cAMP levels in skeletal muscle for up to 4 h after acute exercise bouts of durations that are known to either produce (60 min) or not produce (10 min) mitochondrial proliferation after chronic training. Treadmill-acclimatized, but untrained, rats were run at 22 m/min for 0 (control), 10, or 60 min and were killed at various postexercise (0, 0.5, 1, 2, and 4 h) time points. Fast-twitch white and red (quadriceps) and slow-twitch (soleus) muscles were quickly excised, frozen in liquid nitrogen, and assayed for cAMP with a commercial kit. Unexpectedly, cAMP contents in all three muscles were similar to control (nonexercise) at most (21 of 30) time points after a single 10- or 60-min run. Values at 9 of 30 time points were significantly different from control (P single bout of endurance-type exercise is not, by itself, associated with exercise duration.

  18. Characterization of cAMP accumulation mediated by three α1—adrenoceptor subtypes in HEK293 cells

    Institute of Scientific and Technical Information of China (English)

    SONGYao; HUANGYan; DongEr-Dan; HANQi-De; ZHANGYou-Yi


    AIM:To investigate the characterization of cAMP response mediated by α1-adrenoceptor (α1-AR) subtypes in HEK293 cells. METHODS:(1) Full-length cDNA encoding three α1-AR subtypes were transfected into HEK293 cells by the calcium phosphate precipitation method, respectively. (2) The densities of α1-AR subtypes expressed in HEK293 cells were measured by radioligand binding assay. (3)cAMP accumulation was measured by [3H] adenine prelabeling method. RESULTS: (1)Activation of each of three subtypes resulted in an increase of cAMP accumulation in HEK293 cells in a dose-dependent manner, which was inhibited by selective α1-AR antagonist prazosin. (2) Comparing the pharmacological property, the maximal responses of α1A-AR to agonists were the most potent, while the sensitivity of α1-AR subtypes to norepinephrine(NE) was the highest. CONCLUSION: Each of three α1-AR subtypes can mediate cAMP accumulation in HEK293 cell line, and there are differences in pharmacological property.

  19. Social Skills Development in Children with Attention Deficit/Hyperactivity Disorder through Participation in a Residential Summer Camp Program. (United States)

    Guettal, Zoe; Potter, Tom


    A study assessed social skills development in children aged 8-13 with attention deficit/hyperactivity disorder after they attended a residential summer camp. Questionnaires returned by parents of 12 such campers reported increases in age-appropriate behavior, independence, self-esteem, connection with peers, and participation in group activities.…

  20. 77 FR 31395 - The Temporary Labor Camps Standard; Extension of the Office of Management and Budget's (OMB... (United States)


    ... the number of migrant workers from 135,830 to 109,760 workers. Additionally, based upon this new data... Occupational Safety and Health Administration The Temporary Labor Camps Standard; Extension of the Office of...: Occupational Safety and Health Administration (OSHA), Labor. ACTION: Request for public comments. SUMMARY:...

  1. Professional Development of Elementary and Science Teachers in a Summer Science Camp: Changing Nature of Science Conceptions (United States)

    Karaman, Ayhan


    Many countries all over the world have recently integrated nature of science (NOS) concepts into their science education standards. Providing professional support to teachers about NOS concepts is crucially important for successful implementation of the standards. For this purpose, a summer science camp was offered to elementary and science…

  2. Trauma Healing in Refugee Camps in Guinea: A Psychosocial Program for Liberian and Sierra Leonean Survivors of Torture and War (United States)

    Stepakoff, Shanee; Hubbard, Jon; Katoh, Maki; Falk, Erika; Mikulu, Jean-Baptiste; Nkhoma, Potiphar; Omagwa, Yuvenalis


    From 1999 to 2005, the Minneapolis-based Center for Victims of Torture (CVT) served Liberian and Sierra Leonean survivors of torture and war living in the refugee camps of Guinea. A psychosocial program was developed with 3 main goals: (1) to provide mental health care; (2) to train local refugee counselors; and (3) to raise community awareness…

  3. Gene Expression Patterns Define Key Transcriptional Events InCell-Cycle Regulation By cAMP And Protein Kinase A

    Energy Technology Data Exchange (ETDEWEB)

    Zambon, Alexander C.; Zhang, Lingzhi; Minovitsky, Simon; Kanter, Joan R.; Prabhakar, Shyam; Salomonis, Nathan; Vranizan, Karen; Dubchak Inna,; Conklin, Bruce R.; Insel, Paul A.


    Although a substantial number of hormones and drugs increase cellular cAMP levels, the global impact of cAMP and its major effector mechanism, protein kinase A (PKA), on gene expression is not known. Here we show that treatment of murine wild-type S49 lymphoma cells for 24 h with 8-(4-chlorophenylthio)-cAMP (8-CPTcAMP), a PKA-selective cAMP analog, alters the expression of approx equal to 4,500 of approx. equal to 13,600 unique genes. By contrast, gene expression was unaltered in Kin- S49 cells (that lack PKA) incubated with 8-CPTcAMP. Changes in mRNA and protein expression of several cell cycle regulators accompanied cAMP-induced G1-phase cell-cycle arrest of wild-type S49 cells. Within 2h, 8-CPT-cAMP altered expression of 152 genes that contain evolutionarily conserved cAMP-response elements within 5 kb of transcriptional start sites, including the circadian clock gene Per1. Thus, cAMP through its activation of PKA produces extensive transcriptional regulation in eukaryotic cells. These transcriptional networks include a primary group of cAMP-response element-containing genes and secondary networks that include the circadian clock.

  4. An Investigation of the Role of Learning Conversations in Youth's Authoring of Science Identities during an Informal Science Camp (United States)

    Riedinger, Kelly; McGinnis, J. Randy


    In this investigation, we examined how youth during learning conversations at an informal science education camp negotiated and authored identities as learners of science. Using identity theory as our analytical lens, we investigated by application of qualitative methodology the socially constructed nature of youth's identities. We focused on the…

  5. Virodhamine and CP55,940 modulate cAMP production and IL-8 release in human bronchial epithelial cells

    NARCIS (Netherlands)

    Gkoumassi, E.; Dekkers, B. G. J.; Droege, M. J.; Elzinga, C. R. S.; Schmidt, M.; Meurs, H.; Zaagsma, J.; Nelemans, S. A.


    Background and purpose: We investigated expression of cannabinoid receptors and the effects of the endogenous cannabinoid virodhamine and the synthetic agonist CP55,940 on cAMP accumulation and interleukin-8 (IL-8) release in human bronchial epithelial cells. Experimental approach: Human bronchial e

  6. Impact of drug awareness and treatment camps on attendance at a community outreach de-addiction clinic

    Directory of Open Access Journals (Sweden)

    Om Prakash Giri


    Full Text Available Background: Substance misuse is an increasing problem in urban and rural India. The utility of community-based interventions and preventive strategies are increasingly emphasized in this context. The drug de-addiction and treatment center, Department of Psychiatry, Postgraduate Institute of Medical Education and Research, has been running a drug de-addiction and treatment clinic at Kharar Civil Hospital, Kharar, District Mohali, Punjab, since 1998. As part of an effort to enhance this community outreach program, community-based drug awareness and treatment camps have been organized since March 2004 in villages in and around Tehsil Kharar of Mohali. Aim: To study the impact of the drug awareness and treatment camps on the attendance of patients at the community outreach drug de-addiction and treatment clinic at Kharar Civil Hospital. Methods: Sociodemographic and clinical variables, including treatment outcome-related variables, of patients attending the clinic at Kharar Civil Hospital, before and after the camps were compared. Discussion and Conclusion: The study showed a positive impact on drug awareness and treatment camps held in the community on outpatient attendance at a community outreach clinic, with attendance increasing more than 1.8 times.

  7. 75 FR 30850 - Final Supplementary Rules for Camping on Undeveloped Public Lands in Montana, North Dakota, and... (United States)


    ... Recreation Planner, BLM Montana State Office, 5001 Southgate Drive, Billings, MT 59101-4669. FOR FURTHER... Drive, Billings, Montana 59101-4669, (406) 896-5038. SUPPLEMENTARY INFORMATION: I. Background II... at 72 FR 19958 (April 20, 2007). The rules in the Notice of Camping Limits on Public Lands in...

  8. Master Gardener-Led Lessons Increase Knowledge in Gardening and Environmental Science for Iowa Summer Camp Youth (United States)

    Black, Bruce J.; Haynes, Cynthia; Schrock, Denny; Duerfeldt, Kevin; Litchfield, Ruth


    Gardening and nutrition lessons for children can affect knowledge, actions, and behaviors that support more healthful lifestyles. The objective of the study described in this article was to determine the effectiveness of a master gardener--led education program for youth at a week-long summer camp in Iowa. Garden knowledge was assessed via a…

  9. At-Risk Boys' Social Self-Efficacy and Physical Activity Self-Efficacy in a Summer Sports Camp (United States)

    Su, Xiaoxia; Xiang, Ping; McBride, Ron E.; Liu, Jiling; Thornton, Michael A.


    This study examined at-risk boys' social self-efficacy and physical activity self-efficacy within Bandura's self-efficacy framework. A total of 97 boys, aged between 10 and 13 years, attending a summer sports camp completed questionnaires assessing their social self-efficacy, physical activity self- efficacy, prosocial behaviors, and effort.…

  10. Inhibin alpha gene expression in human trophoblasts is regulated by interactions between TFAP2 and cAMP signaling pathways. (United States)

    Depoix, Christophe L; Debiève, Frédéric; Hubinont, Corinne


    Inhibin α (Inha) gene expression is regulated, in rat granulosa cells, via a cyclic 3',5'-adenosine monophosphate (AMP)-response element (CRE) found in a region of the promoter that is homologous to the human INHA promoter. We previously found that during in vitro cytotrophoblast differentiation, human INHA gene expression was regulated by TFAP2A via association with an AP-2 site located upstream of this CRE. The aim of this study was to evaluate if the human INHA gene was also regulated by cAMP in trophoblasts, and to investigate the possible crosstalk between TFAP2 and cAMP signaling pathways in the regulation of INHA gene expression. Treatment with cAMP or forskolin increased INHA mRNA expression by 7- and 2-fold in primary cytotrophoblasts and choriocarcinoma-derived BeWo cells, respectively. Treatment with the protein kinase A inhibitor H-89 reduced forskolin-induced luciferase activity by ∼40% in BeWo cells transfected with an INHA promoter-driven luciferase reporter vector. TFAP2 overexpression increased basal luciferase activity, whereas the dominant repressor KCREB abolished it. Surprisingly, mutation of the CRE also eliminated the TFAP2-induced transcription, although TFAP2 overexpression was still able to increase forskolin-induced luciferase activity when the AP-2 binding site, but not the CRE site, was mutated. Thus, INHA gene expression is upregulated by cAMP via CRE in human trophoblasts, and TFAP2 regulates this expression by interacting with CRE.

  11. A Randomized Evaluation of the Maryland Correctional Boot Camp for Adults: Effects on Offender Antisocial Attitudes and Cognitions (United States)

    Mitchell, Ojmarrh; MacKenzie, Doris L.; Perez, Deanna M.


    This research addresses the question: Does the military atmosphere of a treatment-oriented boot camp lead to greater reductions in antisocial attitudes and cognitions than a standard correctional facility that is also treatment-oriented? A self-report measure of antisocial attitudes and cognitions was collected from 118 inmates randomly assigned…

  12. Effect of ovine luteinizing hormone (oLH) charge isoforms on VEGF and cAMP production. (United States)

    Montero-Pardo, Arnulfo; Diaz, Daniel; Olivares, Aleida; González-Padilla, Everardo; Murcia, Clara; Gómez-Chavarín, Margarita; Gutiérrez-Ospina, Gabriel; Perera-Marín, Gerardo


    Although an increase in VEGF expression and synthesis in association with LH has been established; it is unknown if all LH isoforms act similarly. This study evaluated the production of cAMP and VEGF among LH isoforms in two in vitro bioassays. The LH was obtained from hypophyses and the group of isoforms was isolated by chromatofocusing. cAMP production was assessed using the in vitro bioassay of HEK-293 cells and VEGF production was evaluated in granulosa cells. Immunological activity was measured with a homologous RIA. Immunoactivity and bioactivity for each isoform were compared against a standard, by estimating the IC50 and the EC50. The basic isoforms were more immunoactive than the standard. The neutral and the moderately acidic had an immunological activity similar to the standard. The acidic isoform was the least immunoreactive. cAMP production at the EC50 dose was similar among the basic isoforms, the moderately acidic and the standard; for the neutral and the acidic, the EC50 dose was higher. It was observed that compared with the control, VEGF production at the lowest LH dose was no different in the standard and each isoform. In the intermediate dose, a positive response was caused in the standard and the neutral and basic isoforms. Although the acidic isoform showed a dose-dependent response, it was not significant relative to the control. In conclusion, the basic isoform generated the greatest cAMP and VEGF production, similar to the reference standard, and the acidic the smallest.

  13. The Effect of Informal and Formal Interaction between Scientists and Children at a Science Camp on Their Images of Scientists (United States)

    Leblebicioglu, Gulsen; Metin, Duygu; Yardimci, Esra; Cetin, Pinar Seda


    A number of studies have already investigated children's stereotypical images of scientists as being male, old, bald, wearing eyeglasses, working in laboratories, and so forth. There have also been some interventions to impose more realistic images of scientists. In this study, a science camp was conducted in Turkey with a team of scientists…

  14. Effects of a Summer Camp Program on Enhancing the Academic Achievement Skills of Children with Autism Spectrum Disorders (United States)

    Davenport, Teresa L.


    The purpose of this study was to determine if a summer camp utilizing academic and behavioral remediation programming could increase the academic achievement of children with autism spectrum disorders. Academic achievement was measured using the Wide Range Achievement Test-Fourth Edition (WRAT4; Wilkinson & Robertson, 2006) and an Informal…

  15. Effects of the STAR Intervention Program on Interactions between Campers with and without Disabilities during Inclusive Summer Day Camp Activities (United States)

    Boyd, Christina M.; Fraiman, Jeffrey L.; Hawkins, Kelly A.; Labin, Jennifer M.; Sutter, Mary Beth; Wahl, Meghan R.


    The purpose of this study was to examine the effects of a peer intervention program designed to increase interactions between children with and without disabilities in an inclusive summer camp. A multiple probe single subject design was used to determine the effects of the STAR intervention on six dyads of campers aged five through ten over two…

  16. Distributed Cognition Minicourse: An Instructional Intervention to Improve the Effectiveness of One-Day Environmental Education Camps (United States)

    Broyles, Iris A.


    This study evaluated the effectiveness of a distributed cognition hybrid minicourse in increasing a 1-day camp's ability to effect long-term knowledge retention and pro-environment attitudes and behaviors in sixth graders. The preevent-postevent minicourse was designed to reduce cognitive overload generated by an intense immersion into the…

  17. Impact of Participating in a Short-Term Intervention Model of Sports Education Camps for Children with Visual Impairments (United States)

    Mc Mahon, John M.


    This three-paper format dissertation explores three topics relevant to participating in a short-term model Sports Education Camp for youth with vision impairments. The three papers are independent studies, yet build upon each other by first measuring physical performance in certain skills, then exploring their levels of self-perception, body mass…

  18. Ammonia differentially suppresses the cAMP chemotaxis of anterior-like cells and prestalk cells in Dictyostelium discoideum

    Indian Academy of Sciences (India)

    Ira N Feit; Erika J Medynski; Michael J Rothrock


    A drop assay for chemotaxis to cAMP confirms that both anterior-like cells (ALC) and prestalk cells (pst cells) respond to cAMP gradients. We present evidence that the chemotactic response of both ALC and pst cells is suppressed by ammonia, but a higher concentration of ammonia is required to suppress the response in pst cells. ALC show a chemotactic response to cAMP when moving on a substratum of prespore cells in isolated slug posteriors incubated under oxygen. ALC chemotaxis on a prespore cell substratum is suppressed by the same concentration of ammonia that suppresses ALC chemotaxis on the agar substratum in drop assays. Chemotaxis suppression is mediated by the unprotonated (NH3) species of ammonia. The observed suppression, by ammonia, of ALC chemotaxis to cAMP supports our earlier hypothesis that ammonia is the tip-produced suppressor of such chemotaxis. We discuss implications of ammonia sensitivity of pst cells and ALC with regard to the movement and localization of ALC and pst cells in the slug and to the roles played by ALC in fruiting body formation. In addition, we suggest that a progressive decrease in sensitivity to ammonia is an important part of the maturation of ALC into pst cells.

  19. Taking Flight: Adolescent Girls' Camps in Lok Jumbish of Rajasthan, India. Education for All: Making It Work. Innovation Series. (United States)

    United Nations Educational, Scientific and Cultural Organization, Bangkok (Thailand). Principal Regional Office for Asia and the Pacific.

    The Education for All: Making It Work series is a major international interagency program designed to collect, analyze, and promote successful basic education projects in the least developed and developing countries. This project report describes the innovative work being undertaken in adolescent girls' camps in Lok Jumbish (Rajasthan, India).…

  20. Kinetic Super-Resolution Long-Wave Infrared (KSR LWIR) Thermography Diagnostic for Building Envelopes: Camp Lejeune, NC (United States)


    15-18 31 frame relative to the door reference described previously. The mean emis - sive cracks were assigned an estimated value of 0.14...11, Camp Lejeune. ERDC/CERL TR-15-18 88 The wall to the left of the door at timestamp 322:07 has a number of emis - sive hot spots, as