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Sample records for camarones silvestres litopenaeus

  1. Descripcion, intensidad de infeccion y prevalencia de metacestodos Lecanicephallidae en camarones peneidos silvestres del nororiente de Venezuela

    National Research Council Canada - National Science Library

    Aguado Garcia, Nieves; Bashirullah, Abul

    2011-01-01

    Las especies de camarones peneidos: Farfantepenaeus brasiliensis, F. notialis, F. subtilis y Litopenaeus schmitti de la region nororiental de Venezuela fueron examinados en busca de cestodos, determinandose la intensidad de infeccion media...

  2. Fuentes de Carbohidratos no tradicionales y su efecto en la digestibilidad de dietas para camarones litopenaeus vannamei

    OpenAIRE

    Gómez,Mauricio; Molina, César

    2001-01-01

    Fuentes de carbohidratos no tradicionales y su efecto en la digestibilidad de dietas para camarones Litopenaeus vannamei Hasta la fecha los alimentos para organismos acuáticos han sido generalmente evaluados en términos de crecimiento y de composición corporal de los animales, confiriendo poca atención a la digestibilidad de los ingredientes o de las dietas compuestas y mucho menos a las posibles interacciones que pueden darse entre niveles de nutrientes presentes en la dieta.

  3. Identificación de protozoaríos parásitos en camarones cultivados del género litopenaeus, colectados en la provincia del Guayas - Ecuador, empleando dos técnicas distintas de histopatología

    OpenAIRE

    L., Daqui

    1999-01-01

    Identificación de protozoarios parásitos en camarones cultivados del género Litopenaeus, colectados en la provincia del Guayas - Ecuador, empleando dos técnicas distintas de histopatología En los primeros meses de 1998, un grupo de epicomensales se estaban desarrollando de manera descontrolada y evitando el crecimiento de camarones litopeneidos en algunos casos o elevando las tasas de mortalidad semanal en otras facilidades.

  4. Evaluacion in vivo del efecto cicatrizante de un gel a base de quitosano obtenido de exoesqueleto de camaron blanco Litopenaeus vannamei

    National Research Council Canada - National Science Library

    Martinez Sanchez, Heimy Franceline; Escobedo Lozano, Amada Yeren; Mendez-Gomez, Evaristo; Vazquez, Alfredo Emmanuel; de Jesus Sol Hernandez, Manuel; Osuna Lizarraga, Anahi Elizabeth

    2014-01-01

    .... Para atender esta problematica, se propone el uso de un gel de quitosano, obtenido del exoesqueleto de camaron ya que presenta actividad antimicrobiana e inmunologica acelerando la cicatrizacion...

  5. Distribucion de Vibrio spp. en agua y sedimento de estanques productores de camaron Litopenaeus vannamei cultivados con agua del lago de Maracaibo

    National Research Council Canada - National Science Library

    Gabriela Suarez, Maria; Medina, Zoraida; Montiel, Marynes; Ibarra, Jose; Salcedo, Astrid

    2015-01-01

    La camaronicultura es uno de los sectores de la acuicultura de mas rapido crecimiento a orillas del Lago de Maracaibo, Venezuela, siendo la especie Litopenaeus vannamei, una de las de mayor produccion...

  6. Influencia de los parámetros ambientales en el crecimiento de camarones Litopenaeus en el sistema de cultivo semi-intensivo, en la granja " Campa s.a."

    Directory of Open Access Journals (Sweden)

    Pablo Guerrero

    1999-12-01

    Full Text Available La influencia de parámetros ambientales (físico-químicos en el desarrollo de camarones Litopeneidos cultivados en el período seco, en una granja cuyo sistema productivo es semi-intensivo, se muestra en las variaciones de su crecimiento. La salinidad el parámetro de mayor influencia, dado que sus fluctuaciones resultaron estadísticamente significativas en tres de los cuatro estanques de producción de camarones de exportación, muestreados en este estudio. La salinidad resultó ser el factor ambiental que mejor explica las variaciones del crecimiento de camarones cultivados (95.4%« c 99.3%, 0.005 c P 0.099, funcionando como parámetro de predicción del crecimiento de ésta especie. El estudio se realizó en la empresa productora de Camarones Camarones del Pacífico S.A (CAMPA S.A, el período comprendido entre octubre/ 1996 a marzo/ 1997.

  7. Comparison of some reproductive characteristics of farmed and wild white shrimp males Litopenaeus vannamei (Decapoda: Penaeidae

    Directory of Open Access Journals (Sweden)

    Sergio Rendón Rodríguez

    2007-03-01

    Full Text Available We rated some reproductive characteristics of white shrimp Litopenaeus vannamei (Boone, 1931 males using 46 farmed individuals (weighing 21.42±0.56 g and 40 wild individuals (weighing 36.10±0.72 g. In farmed shrimps, spermatophore mean weight was 8.94±0.51 mg; total mean sperm count was 3.90±0.27 x 10(6 in each spermatophore; and mean percentage of normal sperm was 86.9±0.37 %. In wild individuals, the respective values were 30.68±2.32 mg; 6.22±1.09 x 10(6; and 62.1±3.56 %. In both groups, the differences between right and left spermatophore were not significant (pResumen Se evaluaron características reproductivas como peso del espermatóforo, número de espermatozoides y porcentaje de espermatozoides normales en 86 organismos de camarón blanco (Litopenaeus vannamei. de éstos, 46 individuos provinieron de estanques de cultivo con un peso de 21.42±0.56 g y el resto, 40, silvestres con un peso de 36.10±0.72 g. En los camarones cultivados, el peso de los espermatóforos fue de 8.94±0.51 mg; el número total de espermatozoides en cada espermatóforo fue de 3.90±0.27 x 10(6; y el porcentaje de espermatozoides normales fue de 86.9±0.37 %. En los silvestres, los valores respectivos fueron de 30.68±2.32 mg; 6.22±1.09 x 10(6; y 62.1±3.56 %. En ambos grupos, las diferencias entre los espermatóforos derecho e izquierdo no fueron estadísticamente diferentes (p<0.01. Las diferencias en el peso de los espermatóforos y el porcentaje de espermatozoides normales, entre los camarones cultivados y silvestres, fueron significativas (p<0.01; sin embargo, en el número de espermatozoides tales diferencias no fueron significativas (p<0.01. La relación entre el peso del espermatóforo (Ws y el peso de los individuos (Wo fue Ws (mg=1.23(Wo-17.34 (r²=0.89, en camarones cultivados; y Ws (mg=2.57(Wo-60.04 (r²=0.64, en los silvestres. En organismos cultivados, la relación entre el número de espermatozoides (Cs y el peso de los individuos (Wo fue

  8. FAUNA SILVESTRE

    Directory of Open Access Journals (Sweden)

    Luis Fernando Osorio, Director 5 Simp. For.

    2012-10-01

    Full Text Available Comité científico del simposio Director Luis Fernando Osorio Vélez, Ph.D. Universidad Nacional de Colombia, sede Medellín. Colombia. Fauna silvestre Brian C. Bock, Ph.D. Universidad de Antioquia. Colombia. Jaime Polanía Vorenberg, Ph.D. Universidad Nacional de Colombia. Colombia. Joan Gastón Zamora Abrego, Ph.D. Universidad Nacional de Colombia. Colombia. Néstor Javier Mancera Rodríguez, Ph.D. Universidad Nacional de Colombia. Colombia. Sergio Solari, Ph.D. Universidad de Antioquia. Colombia.

  9. FAUNA SILVESTRE

    OpenAIRE

    Luis Fernando Osorio, Director 5 Simp. For.

    2012-01-01

    Comité científico del simposio Director Luis Fernando Osorio Vélez, Ph.D. Universidad Nacional de Colombia, sede Medellín. Colombia. Fauna silvestre Brian C. Bock, Ph.D. Universidad de Antioquia. Colombia. Jaime Polanía Vorenberg, Ph.D. Universidad Nacional de Colombia. Colombia. Joan Gastón Zamora Abrego, Ph.D. Universidad Nacional de Colombia. Colombia. Néstor Javier Mancera Rodríguez, Ph.D. Universidad Nacional de...

  10. Control de la reproducción de camarones marinos

    Directory of Open Access Journals (Sweden)

    Jorge Alfaro Montoya

    2016-03-01

    Full Text Available El entendimiento de la biología reproductiva básica es necesario para el mejoramiento de la producción de gametos de camarones. Esta presentación revisará nuestro conocimiento actual en materia de determinación sexual, control hormonal de la maduración, fertilización, y técnicas para la producción comercial de gametos y desove. La maduración controlada de camarones es una actividad comercial; sin embargo, no se conoce el modelo genético de los sexos, ni se ha desarrollado la reversión de sexos y producción de monosexos. La fertilización in vitro de Penaeus es aun una técnica de bajos rendimientos, y la criopreservación de embriones o larvas no existe. La ablación ocular continúa siendo la técnica de manejo hormonal para la maduración de hembras, aunque la aplicación de serotonina podría ser una alternativa en el futuro cercano. El avance logrado en producción de espermatóforos y en el entendimiento de los problemas asociados con su calidad, son nuevas contribuciones para el mejoramiento de la reproducción controlada de camarones.

  11. Comercio de fauna silvestre en Colombia

    National Research Council Canada - National Science Library

    Néstor Javier Mancera Rodríguez; Otto Reyes García

    2008-01-01

      Este trabajo ofrece un panorama sobre las actividades relacionadas con el comercio de bienes derivados de las especies de fauna silvestre en Colombia, abordando el tema desde el desarrollo que ha...

  12. Respuesta inmunomoduladora de la superóxido dismutasa en juveniles de camarón blanco (Litopenaeus vannamei expuestos a inmunoestimulantes

    Directory of Open Access Journals (Sweden)

    2005-01-01

    Full Text Available Se estudió la actividad inmunomoduladora de la manganeso superóxido dismutasa (MnSOD en juveniles de camarón blanco (Litopenaeus vannamei expuestos a diferentes inmunoestimulantes. Organismos cuyo peso varió entre 0.7 y 1.0 g fueron inmersos durante 6 h en soluciones de β-glucano, lipopolisacárido (LPS, fucoidán, y Vibrio penaeicida muerto por calor. Se determinó la actividad enzimática de la MnSOD en los organismos para evaluar si los diferentes inmunoestimulantes utilizados eran capaces de inducir actividad antioxidante. Los inmunoestimulantes probados activaron el sistema inmune de los camarones, mostrando un incremento generalizado en la respuesta antioxidante 48 h después del ensayo. El mayor incremento en la actividad de la enzima (3.2 veces superior al grupo control se registró cuando se utilizó la bacterina. Se expusieron los camarones a V. penaeicida 10 días después de la exposición a los inmunoestimulantes, obteniendo un incremento en la actividad antioxidante de los camarones expuestos previamente a β-glucano, LPS y fucoidán. La respuesta enzimática más alta se obtuvo con los organismos estimulados con β-glucano (2.5 veces superior al control. Este estudio demostró la capacidad de los juveniles de camarón para mejorar la respuesta antioxidante después de ser expuestos a inmunoestimulantes y a una bacteria patógena.

  13. Amendoins silvestres para uso ornamental.

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    Renato Ferraz de Arruda Veiga

    2003-06-01

    Full Text Available Algumas espécies silvestres de amendoim (Arachis spp. gênero Arachis L. (Fabaceae, vêm sendo utilizadas como forração em jardins no Brasil, porém todas com pouca variabilidade já que a distribuição do germoplasma é feita sempre pelos mesmos acessos4. Por outro lado, inúmeras coletas têm sido realizadas, particularmente pela Embrapa Recursos Genéticos e Biotecnologia (Cenargen, disponibilizando acessos até então inacessíveis à pesquisa científica. Em virtude dessa nova disponibilidade e igualmente de híbridos resultantes de pesquisas do Cenargen, organizou-se este trabalho. Foram objeto desta pesquisa cinco espécies: A. glabrata Benth., A. helodes Mart. ex Krapov.& Rigoni, A. pintoi‘Krapov.& W.C.Gregory, A. repens Handro e A. kempff-mercadoi Krapov.,W.C.Gregory & C.E.Simpson, e seis híbridos originados dos paternais: A. appressipila Krapov.&W.C.Gregory, A. paraguariensis Chodat & Hassl., A. pintoi, A. repens e A. vallsi Krapov.& W.C.Gregoryi. O experimento foi desenvolvido no período dos anos agrícolas de 1998 a 2000, na Fazenda Santa Elisa do Instituto Agronômico (IAC, em Campinas (SP, anotando-se o número de flores por planta, a velocidade de desenvolvimento, a capacidade de cobertura do solo, aspectos ornamentais como exuberância das flores e folíolos, coloração e, ainda, sanidade e vigor dos acessos. Os híbridos apresentaram um bom comportamento, porém com ciclo anual, ao passo que os acessos de Arachis kempffmercadoi, A.helodes, A. repens e A. glabrata mostraram-se mais recomendáveis para o uso em jardins por serem perenes. Todos os acessos ficam mais bonitos no verão em razão do período de floração e graças ao verde de sua massa foliar.

  14. Biología de los camarones Peneidos del manglar de Guadalupe

    NARCIS (Netherlands)

    Rojas-Beltrán, Ricardo

    1981-01-01

    Durante el desarrollo de dos campañas preliminares en 1976 hemos podido recolectar ocho especies de Peneidos en el manglar de Guadalupe. Las citaremos en orden de importancia: Penaeus (Melicerlus) aztecus subtilis, P. (M.) brasiliensis, P. (M.) duorarum notialis, P. (Litopenaeus) schmitti,

  15. Microbiota from Litopenaeus vannamei: digestive tract microbial community of Pacific white shrimp (Litopenaeus vannamei)

    National Research Council Canada - National Science Library

    Tzuc, Jaqueline Tuyub; Escalante, Diana Rendíz; Rojas Herrera, Rafael; Gaxiola Cortés, Gabriela; Ortiz, Maria Leticia Arena

    2014-01-01

    Bacteria capable of producing different extracellular enzymes of potential relevance in digestive processes were isolated from the stomach, hepatopancreas and intestine of Pacific white shrimp Litopenaeus vannamei...

  16. Comercio de fauna silvestre en colombia.

    OpenAIRE

    Mancera Rodríguez, Néstor Javier; Reyes García, Otto

    2011-01-01

    Este trabajo ofrece un panorama sobre las actividades relacionadas con el comercio de bienes derivados de las especies de fauna silvestre en Colombia, abordando el tema desde el desarrollo que ha tenido su actividad productiva, el aprovechamiento extractivo, así como la dinámica de su comercio legal e ilegal en el país y el desarrollo y promoción de alternativas productivas sustentadas en su aprovechamiento. Se analizó la información secundaria de entidades como el Ministerio de Ambiente, Viv...

  17. Efecto de Vibrio harveyi en la sobre vivencia de larvas de Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Gabriel Aguirre-Guzmán

    2013-01-01

    Full Text Available Los cultivos acuícolas son de gran relevancia en la alimentación humana, pero crean un medio ambiente artificial que promueve el crecimiento de diferentes especies de bacterias. Las especies del género Vibrio son bacterias de la microflora normal de los camarones peneidos y son también agentes patógenos oportunistas que pueden tomar ventaja de éstos cambios ecológicos generados en los cultivos acuícolas causando diferentes enfermedades, sobrevivencias bajas y pérdidas económicas en la producción de camarón. El objetivo de la presente investigación fue determinar la variación en la sensibilidad, de diferentes subestadios larvarios (nauplio, zoea I-III, misis I-III y en el de postlarva 1, del camarón blanco del Pacifico Litopenaeus vannamei al ser expuestos a tres dosis [103, 105, y 107 unidades formadoras de colonias (UFC ml-1] de V. harveyi, mediante infecciones por inmersión (30 min. Esta especie generó una sobrevivencia baja significativa en las larvas (p < 0,05 solamente en las dosis más altas (105 y 107 UFC ml-1, siendo la última dosis la que presentó los valores más bajos de sobrevivencia. Además, se observó que los subestadios larvales y en el de postlarva 1 fueron más resistentes a V. harveyi al aumentar la edad de los mismos. Esta información tiene gran significado para la industria acuícola, ya que les permite generar estrategias que disminuyan los efectos de V. harveyi y que les permita mejorar el crecimiento y sobrevivencia de las larvas y de postlarva 1 del camarón.

  18. Coeficientes de utilizacion digestiva aparente de materia seca, proteina y aminoacidos esenciales de ingredientes terrestres para el camaron del Pacifico Litopenaeus vannamei (Decapoda: Penaeidae)

    National Research Council Canada - National Science Library

    Terrazas, Martin; Civera, Roberto; Ibarra, Lilia; Goytortua, Ernesto

    2010-01-01

    ...). Los ingredientes evaluados fueron: caseina (CAS), harinas de subproductos avicolas (HSPA) y porcicolas (HSPP), gluten de maiz (GLM) y trigo (GLT), pasta de soya (PS), harinas de sorgo (HS) y trigo (HT). La DAMS y DAP variaron entre 68...

  19. Evidence of Sympatry of Clade A and Clade B Head Lice in a Pre-Columbian Chilean Mummy from Camarones

    Science.gov (United States)

    Boutellis, Amina; Drali, Rezak; Rivera, Mario A.; Mumcuoglu, Kosta Y.; Raoult, Didier

    2013-01-01

    Three different lineages of head lice are known to parasitize humans. Clade A, which is currently worldwide in distribution, was previously demonstrated to be present in the Americas before the time of Columbus. The two other types of head lice are geographically restricted to America and Australia for clade B and to Africa and Asia for clade C. In this study, we tested two operculated nits from a 4,000-year-old Chilean mummy of Camarones for the presence of the partial Cytb mitochondrial gene (270 bp). Our finding shows that clade B head lice were present in America before the arrival of the European colonists. PMID:24204678

  20. Effect of Nile tilapia (Oreochromis niloticus on the growth performance of Pacific white shrimp (Litopenaeus vannamei in a sequential polyculture system Efecto de la tilapia del Nilo (Oreochromis niloticus sobre el crecimiento del camarón blanco del Pacífico (Litopenaeus vannamei, en un sistema de policultivo secuencial

    Directory of Open Access Journals (Sweden)

    Cesar Hernández-Barraza

    2012-11-01

    Full Text Available The present study was carried out at the Environmental Research Laboratory (ERL, University of Arizona, to assess the effect of the addition of Nile tilapia (Oreochromis niloticus, at different densities, on the growth performance of Pacific white shrimp (Litopenaeus vannamei. The growth rate and feed conversion of shrimp, both in polyculture and monoculture, were evaluated. Shrimp-tilapia proportions were 20:8 individuals in Treatment One (T1, 20:4 individuals in Treatment Two (T2 and 20:2 individuals in Treatment Three (T3, while in Treatment Four (T4 shrimp were stocked as a control group with a ratio of 20:0. The experiment lasted for four weeks at 10 ppt water salinity. The shrimp and fish were fed once a day with 8% and 3% of their body weight, respectively, using a 35% protein feed. At the end of the experiment, the average individual weight and best feed conversion ratio were obtained in shrimp polyculture treatment with highest tilapia density 6.08 ± 0.18 g and 1.26 ± 0.01 respectively, while the lowest scores were found in the monoculture treatment with 5.14 ± 0.59 g and 1.35 ± 0.01, respectively (P El presente estudio se llevó a cabo en el Laboratorio de Investigación del Medio Ambiente (ERL. de la Universidad de Arizona, para evaluar el efecto de la adición de la tilapia del Nilo (Oreochromis niloticus a diferentes densidades, en el desempeno del crecimiento del camarón blanco del Pacífico (Litopenaeus vannamei. La tasa de crecimiento y conversión alimenticia del camarón, tanto en policultivo y monocultivo, fueron evaluados. Las proporciones de camarón y la tilapia fueron de 20:8 individuos en el tratamiento uno (T1, 20:4 en el tratamiento dos (T2 y de 20:2 en el tratamiento tres (T3, mientras que en el tratamiento cuatro (T4, únicamente fueron sembrados camarones, participando como grupo control con una relación de 20:0. El experimento se realizó durante cuatro semanas y agua a 10 ppm de salinidad. Los camarones y

  1. Reglamento del comercio internacional de especies amenazadas de fauna silvestre

    OpenAIRE

    Cancela Amigo, Aurora

    2014-01-01

    Treball presentat a l'assignatura d'Ètica i Legislació. Gestió empresarial (102680) La Convención sobre el Comercio Internacional de Especies Amenazadas de Fauna y Flora Silvestres (CITES) es un tratado creado en Washington el 3 de marzo de 1973 ante el creciente problema del cruce de fronteras de especies animales y vegetales de Fauna y Flora Silvestres. Tras muchos años de combate individual, los países llegaron a la conclusión de que era necesaria una cooperación mundial para salvaguard...

  2. Ranino ua uai: Palabra de cosecha de frutas silvestres

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    Hipolito Candre

    2016-01-01

    Full Text Available Este texto, transcrito de una narración del anciano Hipólito Candre, hace un inventario de los frutos silvestres que se encuentran en el monte y cómo eran aprovechados por las antiguas generaciones. También hace referencia a la colecta de miel silvestre, a la pesca con barbasco y trampas y la colecta de ranas comestibles en trampas. En las notas se da la identificación biológica de las especies mencionadas y se agrega información sobre sus épocas de fructificación y otras informaciones recogidas en el campo.

  3. PLAN DE MANEJO ECOTURÍSTICO: ESTUDIO DE CASO ÁREA PROTEGIDA PROVINCIAL CAMARONES, CANTÓN JAMA, MANABÍ

    OpenAIRE

    Neme Yamil Doumet Chilán; Iván Orlando Mendoza Montesdeoca; Consuelo Maribel Mejía Ramos

    2015-01-01

    Esta investigación tuvo como objetivo elaborar un plan de manejo ecoturístico en el área protegida provincial Camarones del cantón Jama. Este estudio forma parte de la estructura de un plan de manejo ejecutado por el Gobierno Provincial de Manabí para la creación de esta área protegida. Fue necesario desarrollar los siguientes estudios y procesos metodológicos: diagnóstico turístico situacional, zonificación, senderización, e implementación de facilidades y señalética turística. Se utilizó...

  4. PRODUCTOS NATURALES COMO ESTIMULADORES DEL SISTEMA INMUNOLÓGICO DE Litopenaeus vannamei, INFECTADO CON Vibrio parahaemolyticus

    Directory of Open Access Journals (Sweden)

    Nelson Pe\\u00F1a-Navarro

    2013-01-01

    Full Text Available El objetivo de este trabajo fue determinar la respuesta inmunológica de Litopenaeus vannamei con manano-oligosacáridos (T1, ajo (T2 y un compuesto de extractos de plantas (T3 después de ser infectados con Vibrio parahaemolyticus. Se realizaron dos bioensayos con una duración de seis (1x106 UFC y catorce (3x106 UFC días en el Centro de Investigación en Ciencias del Mar y Limnología de la Universidad de Costa Rica durante el 2011. Para esta investigación se extrajeron 120 camarones para cada periodo experimental de fincas ubicadas en la Península de Nicoya. Al finalizar la dosificación de los productos se evaluó la ganancia de peso y la conversión alimenticia. Luego de la infección con V. parahaemolyticus se realizaron hemogramas, coagulación, bacteriología de hemolinfa y mortalidad acumulada. Los parámetros inmunológicos no mostraron diferencias estadísticas (P>0,05 entre tratamientos en ningún periodo, sin embargo a los seis días T1 mostró los mejores resultados con 41,07x105 hemocitos/ml; una coagulación de 34,40 s y 4,44x103 UFC/ ml. En el periodo de catorce días T2 obtuvo los mejores valores (55,76x105 hemocitos/ml; una coagulación de 34,20 s y15,4x103 UFC/ml. La mortalidad acumulada se presentó a las diez horas de inoculación, hubo menor cantidad de muertes en T1 y T3 (76,2% a los seis días, mientras que a los catorce días fue para T1 (93,2%. La ganancia de peso y la conversión alimenticia resultaron con diferencias estadísticas (P<0,05 solo para el bioensayo que se extendió por seis días, donde T2 presentó una biomasa de 54,3 g, un incremento en la ganancia de peso de 19,3% y una conversión alimenticia de 1,4.

  5. COMERCIO DE FAUNA SILVESTRE EN COLOMBIA WILDLIFE TRADE IN COLOMBIA

    OpenAIRE

    Néstor Javier Mancera Rodríguez; Otto Reyes García

    2008-01-01

    Este trabajo ofrece un panorama sobre las actividades relacionadas con el comercio de bienes derivados de las especies de fauna silvestre en Colombia, abordando el tema desde el desarrollo que ha tenido su actividad productiva, el aprovechamiento extractivo, así como la dinámica de su comercio legal e ilegal en el país y el desarrollo y promoción de alternativas productivas sustentadas en su aprovechamiento. Se analizó la información secundaria de entidades como el Ministerio de Ambiente, Viv...

  6. Control Hormonal de la Fertilidad en Animales Silvestres

    OpenAIRE

    M P Paredes-Higuera

    2010-01-01

    Las especies silvestres se encuentran en peligro de extinción y se han visto obligadas a tener una vida en cautiverio donde se alteran sus hábitos alimenticios, sociales y las condiciones medioambientales, lo cual afecta el comportamiento reproductivo y la fertilidad. El objetivo principal de la conservación animal es mantener la biodiversidad dado que la remoción o desaparición de una especie puede afectar el funcionamiento del ecosistema global; por esto se ha incrementado el uso de biotecn...

  7. Ecologia comparativa de ectoparasitos em aves silvestres (Palmas, TO)

    OpenAIRE

    Enout, Alexandre Magno Junqueira

    2009-01-01

    Este trabalho teve como objetivo investigar a fauna de ectoparasitos associada às aves silvestres no município de Palmas, estado do Tocantins, Brasil. A área de estudo compreende três Unidades de Conservação. As aves foram capturadas com redes de neblina em Matas de Galeria, inseridas no Bioma Cerrado, entre junho de 2008 e março de 2009. Todas as aves capturadas (exceto beija-flores) foram examinadas quanto à presença de carrapatos, trombiculídeos, malófagos e ácaros de pena. Os ectoparasita...

  8. PLAN DE MANEJO ECOTURÍSTICO: ESTUDIO DE CASO ÁREA PROTEGIDA PROVINCIAL CAMARONES, CANTÓN JAMA, MANABÍ

    Directory of Open Access Journals (Sweden)

    Neme Yamil Doumet Chilán

    2015-10-01

    Full Text Available Esta investigación tuvo como objetivo elaborar un plan de manejo ecoturístico en el área protegida provincial Camarones del cantón Jama. Este estudio forma parte de la estructura de un plan de manejo ejecutado por el Gobierno Provincial de Manabí para la creación de esta área protegida. Fue necesario desarrollar los siguientes estudios y procesos metodológicos: diagnóstico turístico situacional, zonificación, senderización, e implementación de facilidades y señalética turística. Se utilizó manuales de la UICN y Sistema de Áreas de Conservación Provincial (SACP, además programas de georeferenciación y equipos de GPS. Con esto se busca el uso sustentable y la gestión ecoturística de los recursos existentes. Se observa que en los estudios de diagnóstico y zonificación se determina que el área posee una infinidad de recursos naturales y altos indicadores de biodiversidad, se trata de un ecosistema que está en peligro de desaparecer. Es importante la aplicación de un plan de manejo que sirva como línea base para la gestión de los recursos naturales y culturales. La investigación sirvió como fundamento para el análisis, gestión y ejecución de proyectos ecoturísticos que permitirán el desarrollo de actividades vinculadas al turismo y a la sustentabilidad. Los documentos que se generaron estarán a disposición y vinculados a los Ministerios de Ambiente y Turismo.

  9. Respuesta inmune y expresión de genes en el camarón blanco (Litopenaeus vannamei inducida por inmunoestimulantes microbianos

    Directory of Open Access Journals (Sweden)

    Antonio Luna-González

    2013-11-01

    Full Text Available Se realizó un bioensayo de 26 días para evaluar el efecto inmunoestimulante de bacterias ácido lácticas y levaduras (MI, adicionadas en el alimento, en Litopenaeus vannamei. Los tratamientos del bioensayo se realizaron por triplicado: I dieta control (Camaronina®; II MI en alimento, diario; III MI en alimento, cada tres días y; IV MI en alimento, cada seis días. Los camarones sólo eran libres de WSSV. Para el estudio del sistema inmune se hizo un conteo total de hemocitos, se determinó bioquímicamente la concentración de anión superóxido, y la actividad de la fenoloxidasa. También se estudió la expresión semicuantitativa de seis genes del sistema inmune, utilizando la técnica de RT-PCR. No hubo aumento significativo en el crecimiento y la supervivencia, el conteo total de hemocitos, la concentración de la proteína total en plasma y hemocitos, y la concentración del anión superóxido. La actividad de la fenoloxidasa en plasma en el tratamiento IV fue significativamente mayor que en los tratamientos I, II y III. La fenoloxidasa del SLH (proFO en el tratamiento IV fue significativamente mayor que en los tratamientos I y III. La MI provocó una sobreexpresión significativa de los genes que codifican para la profenoloxidasa (tratamiento IV, lisozima (tratamiento III y transglutaminasa (tratamiento II, con respecto a los animales no tratados (control. La mezcla de inmunoestimulantes microbianos puede aumentar la resistencia de L. vannamei contra patógenos en los cultivos.

  10. Induction of ovarian maturation and spawning by combined treatment of serotonin and a dopamine antagonist, spiperone in Litopenaeus stylirostris and Litopenaeus vannamei

    NARCIS (Netherlands)

    Alfaro Montoya, J.; Zuniga, G.; Komen, J.

    2004-01-01

    The study was designed to develop a reliable technique for inducing ovarian maturation and spawning in Litopenaeus stylirostris and Litopenaeus vannamei, as an alternative to the traditional and destructive eyestalk ablation. Two combinations of molecules were evaluated: (a) serotonin (5-HT) at 50

  11. Tráfico y Venta Ilegal de Animales Silvestres en la Ciudad de Cochabamba, Bolivia

    OpenAIRE

    L P Meriles-Treviño; E R Fortón-Rojas

    2008-01-01

    Artículo divulgativo sobre venta ilegal de fauna silvestre en la Ciudad de Cochabamba, Bolívia. Identifica las problemáticas, marco legal, lugares de venta y actividades para mitigar el impacto de esta actividad.

  12. Sensory quality of frozen shrimp Litopenaeus vannamei

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    Inês Maria Barbosa Nunes Queiroga

    2014-09-01

    Full Text Available The objective of this research was to evaluate the sensory quality of the marine shrimp Litopenaeus vannamei grown and stored in a freezer. A sensory analysis consisted of the Quality Index Method (MIQ to review the raw shrimp and Descriptive Analysis (AD in cooked shrimp in samples stored for a period of 90 days, using eight previously trained panelists. Accommodation comprising shrimp filet (100-120 pieces / kg samples were subjected to freezing in liquid nitrogen (- 86 °C, Freezing Tunnel (- 35 °C and Domestic Freezer (- 18 °C. At 0, 30, 60 and 90 days of storage in these freezing systems, determination of pH, water holding capacity (WHC, weight loss during cooking (WLC, shearing force, color, total volatile bases (TVB and thiobarbituric acid reactive substances (TBARS were performed. The attributes manual firmness and softness showed better results in rapid freezing. The color parameters (a *, b * and L *, WHC and WCL were higher at 90 days of storage, no significant losses were observed. Highlighted the strength of greater shear in slowly frozen samples at 90 days, confirming the results reported by the sensory panel. At 90 days of storage, the frozen shrimp showed good sensory quality and physical and chemistry characteristics. The shelf life of this shrimp could be set at about 90 days.

  13. COMERCIO DE FAUNA SILVESTRE EN COLOMBIA WILDLIFE TRADE IN COLOMBIA

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    Néstor Javier Mancera Rodríguez

    2008-12-01

    Full Text Available Este trabajo ofrece un panorama sobre las actividades relacionadas con el comercio de bienes derivados de las especies de fauna silvestre en Colombia, abordando el tema desde el desarrollo que ha tenido su actividad productiva, el aprovechamiento extractivo, así como la dinámica de su comercio legal e ilegal en el país y el desarrollo y promoción de alternativas productivas sustentadas en su aprovechamiento. Se analizó la información secundaria de entidades como el Ministerio de Ambiente, Vivienda y Desarrollo Territorial, las Corporaciones Autónomas Regionales y Autoridades Ambientales Urbanas, el Instituto Colombiano de Desarrollo Rural-INCODER, las Autoridades Policiales, los Institutos de Investigación, el Departamento Administrativo Nacional de Estadística, la Dirección de Impuestos y Aduanas Nacionales-DIAN, el Ministerio de Comercio, Industria y Turismo, y PROEXPORT. entre otras. En Colombia, el comercio de especies de fauna silvestre está centrado principalmente en la extracción de ejemplares de forma ilegal, lo cual ha generado desequilibrios en las poblaciones naturales y ha repercutido en el deterioro de la dinámica de los ecosistemas. El comercio legal de fauna silvestre se basa en la producción de unas pocas especies entre las que se destacan la babilla (Caiman crocodilus, el chigüiro (Hydrochaeris hydrochaeris, cerca de 200 especies de peces ornamentales y en menor medida el lobo pollero (Tupinambis nigropunctatus, la iguana (Iguana iguana, la boa (Boa constrictor, escarabajos (Dynastes hercules y mariposas. En el país no se tiene información exacta sobre el número de incautaciones realizadas en los operativos de control al tráfico ilegal de fauna, y no existe un conocimiento de la dinámica de este comercio ilegal.This work offers a current view on the activities related to the trade of derived from the wildlife species in Colombia, approaching the topic from the development that has had its productive activity

  14. Prevalencia y distribución de los principales agentes etiológicos que afectan los langostinos silvestres en Tumbes, Perú

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    Rubén Alfaro Aguilera

    2011-05-01

    Full Text Available Se determinó la prevalencia y distribución de diferentes agentes patógenos en langostinos silvestres, en la zona de esteros de la Región Tumbes - Perú, entre marzo y diciembre de 2009. Los canales de marea considerados en este estudio fueron: Boca del Río Tumbes, El Alcalde, Jelí, El Bendito, Envidia, Soledad y Algarrobo. Se colectó un total de 1926 langostinos entre juveniles y pre-adultos de las especies Litopenaeus vannamei, L. stylirostris y Farfantepenaeus californiensis. Utilizando la técnica de la PCR, se detectó la presencia de los patógenos NHPB (0,62%, IHHNV (0,31%, BP (1,61% y WSV (2,75%; no se encontró infección por TSV. Las tres especies en estudio fueron positivas a WSV y BP, presentándose la mayor prevalencia de infección por WSV (2,98% en la especie L. stylirostris y por BP (2,66% en L. vannamei. La NHPB fue detectada en las especies L. vannamei y L. stylirostris con 0,77% y 0,43% de prevalencia respectivamente. Se obtuvo una prevalencia de 0,52% para IHHNV en L. vannamei. Las más altas prevalencias de las infecciones por WSV, BP, NHPB e IHHNV se registraron en los canales de marea El Alcalde (10,79%, Algarrobo (4,51%, Envidia (2,26% y Jelí (5,05%. Los datos señalan la presencia constante de diversos patógenos virales y bacterianos en diferentes especies de peneidos y su amplia distribución a lo largo del litoral tumbesino, lo que constituye un riesgo potencial para el desarrollo de la acuicultura en la región, y podría afectar las poblaciones naturales de langostinos.

  15. FLUJO DE GENES ENTRE FRIJOL COMÚN Y SILVESTRE EN COSTA RICA

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    N\\u00E9stor Chaves-Barrantes

    2009-01-01

    Full Text Available Con el objetivo de analizar el flujo de genes entre frijol común silvestre y cultivado, se determinó el porcentaje de alogamia en condiciones naturales de crecimie nto del frijol silvestre y en estación experimental. En el 2004 se evaluó el cruzamie nto natural entre frijol silvestre mexicano G23511A (Phaseolus vulgaris L. y la línea blanca PAN 68, en la Estación Experimental Fabio Baudrit de la Universidad de Costa Ri ca en Alajuela. Para ello se sembraron dos parcelas experimentales; cada una constó de dos bloques de PAN 68 de 10 surcos (15 m de largo y espacia dos 0,6 m. Entre los bloques y en el borde superior de los mismos, se sembraron tres surcos de frijol silvestre como donantes de polen. Se numeraron las hileras y las plantas de cada una en los bloques de frijol blanco. En Quircot de Ca rtago (área donde crece en forma natural P. vulgaris silvestre, en el 2005 y 2006, se sembraron plantas de frijol comercia l (Vainica de Palo a lo largo de seis sitios junto a las poblaciones de frijol silvestre. En ambas localidades la floración de silvestres y comerciales fue coincidente. Se cosechó toda la semilla de los ensayos y se germinó en bandejas, determinando el porcentaje de alogamia por medio del color morado del hip ocotilo, indicativo de aquellas plántulas cruzadas. En Alajuela el porcentaje de cruza varió entre 0,007 y 0,028 %; mie ntras que en Quircot, entre 0 y 0,199 %.

  16. O outro lado do sonho em Morangos Silvestres

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    Eduardo Peñuela Cañizal

    2008-10-01

    Full Text Available Morangos Silvestres é um filme sobre o sonho, a solidão, a vida e a morte. Uma viagem que Isak Born realiza pelo interior de seu ego. A presente análise tenta revelar a construção do sonho na linguagem fí­lmica seguindo os modelos semânticos de metáfora e a visão freudiana dos processos oní­ricos. Parte de um fragmento da sinopse realizadapor Bergman onde se situa o sentido emergente do sonho. Examinar a relação entre o sonho do personagem em relação aos traços psí­quicos do diretor é também objetivo dessa análise.Abstract: Wild Strawberries is a film about dream, loneliness, life and death. A journey toward the center of Isak Born's ego. The present analysis try to show the dream processing in film language following metaphor semantic patterns and Freudian proposal of dreamming processing. The departure point is a frame from Bergman'ssynopsis in which dream emergent meaning lives. The relationship between Isak Borg dream and Bergman psychism is the other point of analysis.

  17. Vertebrados silvestres atropelados na BR 158, RS, Brasil

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    Daniela da Silva Oliveira

    2012-09-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2012v25n4p229   Entre os problemas que ameaçam a fauna silvestre, o atropelamento de animais é uma importante causa de mortalidade para várias espécies. O presente estudo visou identificar as espécies vitimadas por atropelamento em 98km da rodovia BR 158, entre o município de Cruz Alta e o distrito de Val de Serra, município de Júlio de Castilhos, RS e analisar as variações mensais nas taxas de atropelamento e sua correlação com o volume mensal de chuvas. Foram realizadas expedições mensais, entre os meses de abril a setembro de 2007, onde se registrou 61 animais atropelados pertencentes a 15 espécies, com uma taxa de atropelamento de 0,10 ind./km/mês. As espécies com maior número de atropelamentos foram o zorrilho (Conepatus chinga, com 17 indivíduos (28%, seguida pelo graxaim-do-campo (Lycalopex gymnocercus, com sete (11% e o graxaim-do-mato (Cerdocyon thous, com cinco (8%. Não foi encontrada diferença nas taxas de atropelamento entre os meses amostrados, contudo, o mês com maior registro de atropelamentos foi setembro (n=18. Também não foi verificada correlação entre os atropelamentos e a precipitação mensal, no entanto, há uma tendência entre a ocorrência dos atropelamentos com um volume maior de chuvas.

  18. Efecto de la adición de un extracto acuoso de pionilla Lasianthaea podocephala en el cultivo del camarón blanco del Pacífico Litopenaeus vannamei en condiciones de laboratorio

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    Emmanuel Villanueva-Gutiérrez

    2015-11-01

    Full Text Available Se evaluó el efecto de dos concentraciones de un extracto acuoso de la raíz de pionilla (Lasianthaea podocephala Gray, sobre las variables de la calidad del agua, condición fisiológica y parámetros de producción del camarón blanco del Pacífico, Litopenaeus vannamei (Boone, cultivado en condiciones intensivas de laboratorio. Dos tratamientos y un control fueron evaluados por triplicado: T1 (1 mL de extracto por acuario, T2 (3 mL y C (control, 0 mL. No se observó un efecto negativo de los tratamientos sobre los parámetros de la calidad del agua, los cuales estuvieron dentro de rangos aceptables, sin presentar diferencias significativas entre tratamientos (P < 0,05. Algunos de los parámetros de producción tales como la supervivencia, biomasa final y FCA fueron mejores en los tratamientos en que se utilizó el extracto bajo las condiciones experimentales empleadas. La concentración de metabolitos hemolinfáticos, sugiere que los organismos cultivados en los acuarios con extracto tuvieron mejores condiciones, considerando los niveles mayores de proteína y colesterol en su músculo en relación con el control; además los resultados de expresión de genes indican que el extracto podría tener algún efecto inmunoestimulante sobre los camarones. No obstante, se recomienda efectuar estudios adicionales para evaluar y determinar a nivel molecular los ingredientes activos de los tubérculos de raíz de pionilla, para obtener mayor información sobre el uso potencial de este vegetal en la acuacultura.

  19. Los camarones (Crustácea: Decapoda: Nanantia asociados a praderas de Thalassia Testudinum Banks ex koning, en la región de Santa Marta, caribe colombiano Los camarones (Crustácea: Decapoda: Natantia asociados a praderas de Thalassia Testudinum Banks ex koning, en la región de Santa Marta, caribe colombiano

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    Puentes G. Libia Georgina

    1992-09-01

    Full Text Available Bimonthly samplings were made from November 1988 to September 1989 in the bays of Nenguange, Gayraca, Chengue and Santa Marta, in the Colombian Caribbean, in order to inventory and quantify the shrimp populations associated to Thalassia testudinum beds. A suctioning device connected to a net bag with a mesh size of 1 mm was used for collecting. Shrimp populations data related to habitat complexity were quantified. A total of 2185 individuals were collected, 2183 of which belonging to 7 families, 21 genera and 35 species, one mysis stage, and one Peneidae post larva. Although the number of species collected is high, only 9 were considered permanent, 17 occasional and 9 sporadic. The most abundant species were Periclimenes americanus, Alpheus normanni, Latreutes fucorum, Sicyonia laevigata, Hippolyte curacaoensis, A viridari, L. parvulus, Metapenaeopsis goodei, Thor manningi and Processa fimbriata. High variations in the structure and composition of the shrimp populations were found throughout the study; they were related to grass-bed architecture. Con el fin de caracterizar a las poblaciones de camarones asociadas a praderas de Thalassia testudinum, se realizaron muestreos bimensuales durante el período comprendido entre noviembre de 1988 y septiembre de 1989 en las Bahías de Nenguange, Gayraca, Chengue y Santa Marta en el Caribe Colombiano. Para la colecta de camarones se utilizó un succionador, conectado a una bolsa recolectora de red con ojo de malla de 1 mm.Se cuantificaron las poblaciones de camarones y los parámetros relacionados con la complejidad del hábitat. Se colectó un total de 2185 individuos, de los cuales 2183 pertenecen a 7 familias 21 géneros y 35 especies, un estado mysis y una post-larva de Peneidae. Si bien, el número de especies hallado fue alto, solamente 9 se consideraron permanentes, 17 ocasionales y 9 esporádicas. Las especies más abundantes en su orden fueron: Periclimenes americanus, Alpheus normanni

  20. CITES felinos salvajes : (convenio sobre el comercio internacional de especies amenazadas de fauna y flora silvestres) /

    OpenAIRE

    Cabanellas Cabezas, Ana María.

    2011-01-01

    CITES: Convention on International Trade in Endangered Species of Wild Fauna and Flora = Conveni sobre el Comerç Internacional d’Espècies Amenaçades de Fauna i Flora Silvestre. Treball presentat a l'assignatura de Deontologia i Veterinària Legal (21223)

  1. Parásitos de animales silvestres en cautiverio en Lima, Perú

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    Lily Arrojo

    2013-06-01

    Full Text Available Los parásitos de los animales silvestres en cautiverio peruanos se registran aquí. La mayoría de las especies son nematodos. Dos especies son al patógeno de host: Prosthenorchois elegans y Strongyloides sp.

  2. Growth, mortality and migratory pattern of white shrimp (Litopenaeus vannamei, Crustacea, Penaeidae in the Carretas-Pereyra coastal lagoon system, Mexico

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    G Rivera-Velázquez

    2008-06-01

    Full Text Available The growth, mortality and migration pattern of the population of Litopenaeus vannamei Boone 1931 in the Carretas-Pereyra coastal lagoon system, Mexico, were studied. The shrimp spatial distribution and abundance were analyzed in relation to salinity, water temperature, and substrate. A total of 2 669 shrimps was collected at 22 sites sampled monthly from March 2004 to August 2005. Juvenile shrimps of L. vannamei were present in the coastal lagoon system throughout the year, reaching densities from 0.001 to 0.302 ind/m². The estimated daily growth rate was 0.06 to 0.27 mm carapace length (CL. No significant seasonal differences were appreciated. Weekly total mortality (Z was between 0.04 and 0.34. Recruits, juveniles and sub-adults displayed a bimodal distributional pattern regulated by the prevailing conditions during the dry season. The peak abundance of juvenile stages occurred in December-January and March-May. The abundance presented an inverse correlation with salinity (r=-0.42; pSe estudió el crecimiento, la mortalidad y el patrón de migración del camarón Litopenaeus vannamei Boone 1931 en el sistema lagunar costero Carretas Pereyra, México. La distribución espacial y la abundancia fueron analizadas con relación a la salinidad, temperatura y substrato. De marzo de 2004 a agosto de 2005 se recolectó un total de 2669 camarones con un muestreo mensual en 22 sitios. Los jóvenes se hallaron todo el año en el sistema lagunar costero, con densidades entre 0.001 y 0.302 ind/m². La tasa de crecimiento diaria fue de 0.06 a 0.27 mm longitud del cefalotórax (CL y no se apreciaron diferencias significativas entre estaciones. La mortalidad total (Z semanal estuvo entre 0.04 y 0.34. Reclutas, jóvenes y subadultos presentan un patrón de distribución bimodal regulado por las condiciones prevalecientes durante la estación de estío. Los valores máximos de abundancia de los estadios juveniles se presentan en diciembre-enero y marzo-mayo. La

  3. Increasing Calcium Oxide (Cao) to Accelerate Moulting and Survival Rate Vannamei Shrimp (Litopenaeus Vannamei))

    OpenAIRE

    Erlando, Gito; ', Rusliadi; Mulyadi,

    2016-01-01

    The research about the increasing Calcium Oxide (CaO) to accelerate moulting and survival rate vannamei shrimp (Litopenaeus vannamei) was conducted from Agustus until September 2015 at Balai Perikanan Budidaya Air Payau, Instalasi Pembenihan Udang (IPU) Gelung, Situbondo Provinsi Jawa Timur. The purpose of this research was to investigate optimum doses calcium oxide to accelerate moulting and the survival rate vannamei shrimp (Litopenaeus vannamei). Vannamei shrimp with size PL25 were used in...

  4. Resumen de la tesis doctoral Efectos toxicológicos de plaguicidas organofosforados y organoclorados sobre camarones del género Penaeus sp en Sinaloa, México.

    OpenAIRE

    Osuna Flores, Isabel; Galindo Reyes, José Guillermo; Riva Juan, María del Carmen

    1998-01-01

    El presente trabajo tiene como objetivo ampliar los conocimientos sobre la presencia, la toxicidad y los efectos que puedan tener los plaguicidas organofosforados y organoclorados en la Bahía de Ohuira, Topolobampo, Sinaloa, Mexico. Para ello se ha realizado el análisis de residuos de dichos plaguicidas, en agua superficial, sedimento y camarones del género Penaeus sp. Se han realizado tratamientos agudos y subagudos con dichos compuestos y se han evaluado los efectos toxicológicos a travé...

  5. ZOONOSIS TRANSMITIDAS POR ANIMALES SILVESTRES Y SU IMPACTO EN LAS ENFERMEDADES EMERGENTES Y REEMERGENTES

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    Santiago Monsalve B

    2009-08-01

    Full Text Available Las enfermedades emergentes y reemergentes originadas desde animales de vida silvestre, pueden ser transmitidas a las poblaciones humanas por contacto directo o por vectores. Las zoonosis determinan una gran problemática social epidemiológica. La relación que tiene las enfermedades emergentes y reemergentes está ligada a la pérdida de la biodiversidad por factores antropogénicos, debido a la destrucción de hábitats naturales, el tráfico de fauna y a la pérdida de diversidad genética. Todos estos aspectos juegan un papel en la aparición de las patologías de origen infeccioso. Esta revisión intenta acercarse al conocimiento de las zoonosis transmitidas por animales silvestres y su impacto en las enfermedades emergentes y reemergentes.

  6. Tuberculosis de la fauna silvestre en ganaderías de bovino de lidia (Bos Taurus)

    OpenAIRE

    Miralles, Alberto; Mas, A.; Rosa, A.; Seva, J.I.

    2011-01-01

    La tuberculosis es una enfermedad infecciosa causada por microorganismos del Complejo Mycobacterium tuberculosis (CMTB), principalmente compuesto por Mycobacterium bovis y Mycobacterium caprae. En el presente estudio se determinará la presencia de tuberculosis en dos especies de fauna silvestre: el jabalí y el ciervo, los cuales viven en contacto con ganado bovino de lidia. Para ello se estudiaron un total de 32 jabalíes y 8 ciervos, abatidos en dos monterías celebradas en fincas ...

  7. [Reseñas Bibliográficas] Atlas de la flora vascular silvestre de Burgos

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    Iñaki Aizpuru

    2005-01-01

    Full Text Available Es una reseña bibliográfica de la obra, Atlas de la flora vascular silvestre de Burgos / Alejandre Sáenz, J.M. García-López, L. Marín Padellano, G. Mateo Sanz, E. Miguélez del coso, C. Molina Martín, G. Montamarta Prieto, S. Patino Sánchez, M.A. Pinto Cebrián 6 J. Valencia Janices.

  8. Mamíferos silvestres atropelados na rodovia rs-135 e entorno

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    Carla Grasiele Zanin Hegel

    2012-01-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2012v25n2p165 Dentre os impactos ambientais, a fragmentação dos hábitats para uso agrícola e pecuário, tem provocado a descaracterização natural dos ambientes e como consequência, o atropelamento da fauna silvestre nas rodovias. Foram realizadas vistorias semanais ao longo da rodovia RS-135 (km 08 ao 34, entre maio de 2008 e maio de 2010. Os animais atropelados foram coletados e tombados na coleção de mamíferos da Universidade de Passo Fundo (CMUPF, onde tiveram o conteúdo estomacal removido para análise. Foram encontradas 16 espécies de mamíferos silvestres atropelados, distribuídas em 12 famílias, totalizando 95 animais coletados, com uma freqüência de 0,012 espécimes por quilômetro percorrido. As espécies mais abundantes foram Cerdocyon thous (22,1%, Nasua nasua (10,5%, Pseudalopex gymnocercus (9,5% e Cavia aperea (7,4%. Estes atropelamentos juntos representam 49,5% das ocorrências. Este estudo teve por objetivo identificar as espécies atropeladas no trecho da rodovia e contribuir com informações da biologia da mastofauna silvestre do Rio Grande do Sul. Palavras-chave: Atropelamentos, vertebrados silvestres, conteúdo estomacal, conservação.

  9. Adiciones y revisiones al Atlas de la flora vascular silvestre de Burgos

    OpenAIRE

    Alejandre Sáez, Juan Antonio; Arán Redó, Vicente J.; Pablo BARBADILLO ESCRIVÁ DE ROMANÍ; Juan José BARREDO PÉREZ; Javier BENITO AYUSO; Escalante Ruiz, María Josefa; García López, Javier María; Luis MARÍN PADELLANO; Gonzalo MATEO SANZ; Moreno Moral, Gonzalo; Alberto RODRÍGUEZ GARCÍA; Sánchez Fernández, Rafael

    2016-01-01

    Se mencionan 31 táxones con citas y/o comentarios referidos a su existencia en la provincia de Burgos. De ellos 8 suponen una novedad para el catálogo provincial. Additions and revisions for the “Atlas de la flora vascular silvestre de Burgos”, IX. 31 Taxa with either quotations or remarks, related to their existence within the province of Burgos, are mentioned. 8 out of these aforementioned ones mean a novelty value for the provincial catalogue.

  10. DESTINO DOS ANIMAIS SILVESTRES RECEBIDOS PELO CRIADOURO CONSERVACIONISTA DA UNIVERSIDADE DO VALE DO PARAÍBA

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    Terezinha Fátima Oliveira

    2017-01-01

    Full Text Available A fauna silvestre vem sendo ameaçada pela constante retirada de animais da natureza. Como uma possibilidade de minimizar o problema foram criados empreendimentos que visam a conservação ex situ da fauna, fazendo a recepção, tratamento e reabilitação. O levantamento dos dados a respeito do recebimento e das reintroduções de animais silvestres foi realizado a partir da movimentação do plantel durante os anos de 2012 a 2014. No período amostrado o recebimento de animais totalizou em 3.270 exemplares, sendo 5,44% répteis, 83,42% aves e 11,14% mamíferos. No mesmo período foram reintroduzidos 2.798 animais, sendo 7,58% répteis,  82,02% aves  e 10,40% mamíferos.  Levando-se em consideração os dados apresentados observa-se que foram repatriados na natureza 85,56% dos animais recebidos de 2012 a 2014, indicando um sucesso das reabilitações e na colaboração da conservação das espécies de animais silvestres.

  11. Presença do vírus da síndrome da mancha branca em crustáceos decápodes silvestres em lagoas costeiras no Sul do Brasil

    Directory of Open Access Journals (Sweden)

    S.W. Costa

    2012-02-01

    Full Text Available A presença do vírus da síndrome da mancha branca (em inglês WSSV nas principais espécies de camarões, siris e caranguejos de cinco lagoas que recebem o efluente de fazendas afetadas pela enfermidade foi detectada por nested PCR, e inclusões virais nos camarões por histologia. Pela nested PCR encontrou-se a presença de WSSV em 13 de 16 (81,2% amostras de camarões da espécie Farfantepenaeus paulensis, em 13 de 14 (92,8% de Litopenaeus schmitti, em uma de duas de Farfantepenaeus brasiliensis (50%, em 13 de 15 (86,6% de siri da espécie Callinectes danae e em 11 de 12 (91,6% de Callinectes sapidus, e não foi detectada no caranguejo Chasmagnathus granulata em 10 amostras. Inclusões características de WSSV foram observadas em três amostras histológicas de 50 (6,0% no epitélio gástrico e cuticular e nas brânquias de dois exemplares de F. paulensis e um de L. schmitti. É o primeiro relato da presença de WSSV em camarões L. schmitti e no siri C. danae silvestres. As principais espécies de camarões e siris dos ambientes de entorno das fazendas foram contaminadas pelo WSSV, constituindo-se em vetores potenciais do vírus.

  12. Humoral and Haemocytic Responses of Litopenaeus vannamei to Cd Exposure

    Directory of Open Access Journals (Sweden)

    Juan C. Bautista-Covarrubias

    2014-01-01

    Full Text Available White shrimp, Litopenaeus vannamei, subadults were exposed to four dilutions of the 96 h cadmium LC50 reported for postlarvae (PL12 of this species, and the effects were evaluated after 5, 48, and 96 h of exposure. While treatments did not affect survival and hemolymph clotting time increased with time, but not as a response to Cd exposure, the intensity of other responses was related to concentration, to time of exposure, and to their interaction. Hemocyanin decreased with time in all metal concentrations but increased in the control treatment, and an almost similar trend was observed with hemocyte numbers. As an initial response, phenoloxidase activity decreased with all metal concentrations, but it increased later to values similar or higher than the control treatment.

  13. Hacia el Entendimiento de la Analgesia y Anestesia en Animales Silvestres

    OpenAIRE

    Farnando Nassar-Montoya

    2010-01-01

    La práctica en especies silvestres que lógicamente conlleva la utilización de fármacos analgésicos y anestésicos, presenta una idiosincrasia propia que tiene que ser entendida desde la conceptualización del significado de la medicina veterinaria. Por esto, estos textos inician con una reflexión a partir del siguiente texto de una sentencia del Tribunal Nacional de Ética Profesional de Medicina Veterinaria y Zootecnia de Colombia: “… La investigada se excusó en que realizó el examen clínico ge...

  14. IMPORTANCIA CULTURAL DE LA FLORA SILVESTRE DEL DISTRITO DE PAMPAROMÁS, ANCASH, PERÚ

    OpenAIRE

    Castañeda Sifuentes, Roxana; Departamento de Etnobotánica y Botánica Económica, Museo de Historia Natural, Universidad Nacional Mayor de San Marcos, Av. Arenales 1256, Jesús María, Lima, Perú. Facultad de Ciencias Veterinarias y Biológicas, Universidad Científica del Sur, Av. Antigua Carretera Panamericana Sur Km. 19, Villa El Salvador, Lima, Perú. Autor para correspondencia. Facultad de Ciencias Naturales y Matemática, Universidad Nacional Federico Villarreal, Jr. Río Chepén 290, El Agustino, Lima, Perú.; Albán Castillo, Joaquina; Departamento de Etnobotánica y Botánica Económica, Museo de Historia Natural, Universidad Nacional Mayor de San Marcos, Av. Arenales 1256, Jesús María, Lima, Perú.

    2016-01-01

    El presente estudio etnobotánico se realizó en el distrito de Pamparomás. Los objetivos desarrollados estuvieron referidos en rescatar el conocimiento ancestral asociado a la flora silvestre y determinar las especies de mayor importancia cultural. La metodología aplicada en la investigación se basó en la ejecución de entrevistas informales y semiestructuradas a 202 informantes locales. Los datos de las entrevistas informales fueron obtenidos mediante preguntas abiertas durante las caminatas e...

  15. Uso de la fauna silvestre del Lago de Tota. Peces, herpetos, aves y mamíferos.

    OpenAIRE

    Moncaleano Niño, Angela Margarita; Calvachi Zambrano, Byron Alonso

    2011-01-01

    En este estudio, se realiza una aproximación a la identificación de la fauna silvestre de Lago de Tota (Aquitania-Boyacá), particularmente de los grupos de peces, herpetos, aves y mamíferos. Además, se hacen aportes importantes para el conocimiento de la relación de las comunidades de habitantes aledañas con la fauna silvestre, en cuanto a su uso comercial o artesanal. Se realizaron los listados de las especies presentes en lago para cada uno de los grupos de la fauna, a partir de información...

  16. DIAGNÓSTICO JURÍDICO SOBRE EL DECOMISO Y MANEJO POSTDECOMISO DE FAUNA SILVESTRE EN EL DEPARTAMENTO DEL CHOCÓ

    OpenAIRE

    Lisneider Hinestroza Cuesta; Marisela Mena Valencia; Kelly Johana Palacios Sánchez

    2011-01-01

    En este artículo se presenta un estudio de carácter descriptivo sobre los decomisos y el manejo postdecomiso de fauna silvestre en el departamento del Chocó utilizado como corredor para el tráfico y comercialización ilegal; se analizan, a partir del marco jurídico colombiano, la definición de fauna silvestre, decomiso y sus consecuencias jurídicas. Entre el 2005 y marzo del 2011 se realizaron en el Chocó 904 decomisos: 316 aves, 321 reptiles y 264 de mamíferos, 1 anfibio, 1 arácnido y 1 pez. ...

  17. Efficacy of marine green alga Ulva fasciata extract on the management of shrimp bacterial diseases Eficacia del extracto del alga marina verde Ulva fasciata sobre el manejo de las enfermedades bacterianas en camarones

    Directory of Open Access Journals (Sweden)

    Joseph Selvin

    2011-07-01

    Full Text Available Secondary metabolites of the green algae, Ulva fasciata, were tested to determine the efficacy of controlling shrimp bacterial pathogens. Exploratory experiments indicated that an intermediate dose (1 g kg-1 of shrimp of Ulva in the diet was highly effective at controlling bacterial pathogens of shrimp, as compared to lower (500 mg kg-1 and higher (1.5 g kg-1 doses. The pilot experiments evaluated the percent of relative protection afforded shrimps treated with Ulva diet and faced with various concentrations of bacterial pathogen. The survival of shrimps treated with Ulva diet was significant (P Metabolites secundario de algas verdes Ulva fasciata fue probado para determinar la eficacia de controlar el camarón pathogens bacterial. Las conclusiones de experimentos exploratorios indicaron que la dosis mediana (1 g kg-1 de camarón de dieta Ulva era sumamente eficaz en el control de pathogens bacterial de camarón cuando comparado al más abajo (500 mg kg-1 y más alto (1,5 g kg-1 dosis. En los experimentos pilotos, la protección de pariente de por ciento de camarones trató con la dieta Ulva y desafió con varias concentraciones de bacterial patógeno fueron evaluados. La supervivencia de camarones trató con la dieta Ulva era significativo (P < 0,01. Basado en las conclusiones presentes, podría ser deducido que U. verde fasciata puede ser una fuente excelente para desarrollar la comida potente medicinal para la dirección de enfermedad de camarón.

  18. Análisis histopatológico en Litopenaeus vannamei infectado con Vibrio parahaemolyticus.

    Directory of Open Access Journals (Sweden)

    Nelson Peña-Navarro

    2015-01-01

    Full Text Available El objetivo de este trabajo fue describir los principales resultados histopatológicos obtenidos de las diversas respuestas inmunológicas y efectos citopáticos en L. vannamei, después de una infección inducida con V. parahaemolyticus. La inoculación bacterial se realizó en el Centro de Investigación en Ciencias del Mar y Limnología de la Universidad de Costa Rica, entre junio y agosto del 2011. Para la investigación se utilizaron 40 camarones extraídos de fincas ubicadas en la Península de Nicoya; a cada uno se le inyectó en promedio 2,0x106 UFC de V. parahaemolyticus. Al finalizar el periodo de infección, se realizaron los análisis histopatológicos en branquias, epitelio subcuticular, hepatopáncreas, intestino, órgano linfoide, tejido hematopoyético, músculo estriado, tejido nervioso, corazón, entre otras. Los principales hallazgos a nivel histológico fueron hemocitos melanizados, infiltración hemocítica, melanosis, esferoides tipo A, B y C, mionecrosis coagulativa, fagocitosis, picnosis, entre otras, útiles para determinar el estado sanitario de los camarones y en particular, el efecto citopático de V. parahaemolyticus en infecciones sistémicas.

  19. Composición por tallas, edad y crecimiento de Litopenaeus vannamei (Natantia: Penaeidae, en la laguna Mar Muerto, Oaxaca-Chiapas, México

    Directory of Open Access Journals (Sweden)

    Sebastián Ramos-Cruz

    2000-12-01

    Full Text Available De mayo a noviembre de 1996 se muestreó la captura comercial de camarón blanco (L. vannamei en la laguna Mar Muerto, localizada entre los estados de Oaxaca y Chiapas, México. Con la finalidad de conocer el crecimiento de esta especie en el interior de este sistema lagunar se registraron las tallas y pesos individuales de 16,378 camarones que en conjunto pesaron 68,645 g. Las tallas observadas fluctuaron entre 11 y 145 mm de longitud total, con pesos entre 0.1 y 26.3 g. La talla media mínima de reclutamiento a la fase explotada es de 45 mm. Los organismos abandonan el sistema lagunar para reclutarse a la zona marina con una talla que varía entre 83 y 120 mm, con una edad de1.5 a 4.5 meses. Con el análisis de progresión modal se detectaron 13 generaciones en el interior de la laguna. Las tasas de crecimiento fluctuaron entre 0.13 y 1.27 mm/día con un valor medio de 0.64 mm/día. Las longitudes asintóticas estimadas para cada generación variaron entre 105 y 159 mm, ubicándose la talla media en 136 mm, mientras que el peso asintótico medio fue de 15 g, con valores extremos de 8 y 23 g. Los parámetros del modelo de crecimiento de von Bertalanffy son: L = 136 mm, P = 15 g, k = 0.0161 mm y to = 5 mm. Tanto L como P no representan, al menos en este caso, las tallas y pesos asintóticos que la especie alcanza dentro del sistema lagunar, sino que deben de interpretarse como las tallas y pesos medios que debieran tener los organismos al momento de abandonarlo.The commercial catches of white shrimp Litopenaeus vannamei were sampled (May through November 1996 in the Mar Muerto lagoon located between Oaxaca and Chiapas states, Mexico. To measure it’s growth rate at the innermost portions of this system, 16 378 length and weight records were obtained. Length fluctuated between 11 and 145 mm, and weights between 0.1 and 26.3 g. The minimum mean size for recruitment to the exploited phase is 45 mm. Organisms leave the lagunar system for

  20. Sistemas de extraccion para el aislamiento de esterasas con adsorcion interfacial

    National Research Council Canada - National Science Library

    del Monte Martinez, Alberto; Gonzalez Bacerio, Jorge; Romero del Sol, Lazara; Aragon Abreu, Carlos; Vega Villasante, Fernando; Nolasco Soria, Hector; Diaz Brito, Joaquin

    2009-01-01

    En el presente trabajo se optimizaron las condiciones de extraccion de esterasas con actividad en interfaces, a partir de la anemona marina Stichodactyla helianthus y del camaron peneido Litopenaeus vannamei...

  1. Litopenaeus vannamei notch affects lipopolysaccharides induced reactive oxygen species.

    Science.gov (United States)

    Ning, Pei; Zheng, Zhihong; Aweya, Jude Juventus; Yao, Defu; Li, Shengkang; Ma, Hongyu; Wang, Fan; Zhang, Yueling

    2018-04-01

    Notch signaling pathway was originally discovered in the development stage of drosophila but has recently been found to play essential roles in innate immunity. Most previous studies on Notch have focused on mammals, whereas, in this study, we employed the shrimp Litopenaeus vannamei as a model to study the functions of Notch in invertebrate innate immune system. Our results showed that LvNotch was highly expressed in hemocytes and could be strongly induced by lipopolysaccharides (LPS) injection. Small interfering RNA (siRNA)-mediated knockdown of LvNotch could significantly increase LPS induced L. vannamei mortality, which might be due to the fact that LPS induced ROS was greatly enhanced in LvNotch knockdown shrimps. Further, quantitative polymerase chain reaction (qPCR) analysis revealed that LvNotch could affect the expression of multiple genes, including dorsal, relish, anti-lipopolysaccharide factor 1 (ALF1), ALF3 and NADH dehydrogenases which were upregulated, and Hypoxia-inducible factor (HIF, α/β) which were downregulated in LPS treated shrimps. In summary, LvNotch is important in the control of inflammation-induced ROS production in shrimp. Copyright © 2017 Elsevier Ltd. All rights reserved.

  2. Relation on phitoplankton community with Litopenaeus vannamei productivity in biocrete pond

    Directory of Open Access Journals (Sweden)

    T. Budiardi

    2007-07-01

    Full Text Available ABSTRACTManagement of phytoplankton is generally conducted by controlling the concentration of organic matter, fertilization and water exchange.  Organic materials are from uneaten feed and excretion of shrimp.  By using post facto method it was found four class of phytoplankton in biocrete pond at one cycle rearing of white shrimp (Litopenaeus vannamei.  Population at early rearing period was dominated by Bacillariophyceae (50.4%; 13 species and Cyanophyceae (42.41%; 1 species, followed by Dynophyceae (6.2%; 5 species and Chlorophyceae (1.3%; 1 species.  Increment in phytoplankton density was followed by increment in chlorophyll-a and oxygen from photosynthesis, and productivity was 2132 kg/pond.Keywords: phytoplankton, white shrimp, Litopenaeus vannamei, biocrete pond ABSTRAKPengelolaan fitoplankton umumnya dilakukan dengan mengoptimalkan bahan organik serta pemupukan dan pergantian air. Bahan organik berasal dari pakan buatan yang tidak terkonsumsi (sisa pakan dan ekskresi dari udang. Dengan menggunakan metode post facto selama satu siklus pemeliharaan udang vaname (Litopenaeus vannamei pada tambak biocrete diperoleh empat kelas fitoplankton. Dominasi Bacillariophyceae (50,4%; 13 jenis, Cyanophyceae (42,41%; 1 jenis terjadi pada awal pemeliharaan yang diiukuti oleh Dynophyceae (6,2%; 5 jenis dan Chlorophyceae (1,3%; 1 jenis. Peningkatan kelimpahan fitoplankton secara keseluruhan diikuti oleh peningkatan kandungan klorofil-a dan oksigen hasil fotosintesis total sehingga produktifitasnya mencapai 2132 kg/petakKata kunci: fitoplankton, udang vaname, Litopenaeus vannamei, tambak biocrete

  3. The effects of boiling on the allergenic properties of tropomyosin of shrimp (litopenaeus vannamei).

    Science.gov (United States)

    Shrimp play an important role in human nutrition, and is responsible for severe hypersensitivity reactions. The thermal stability of raw and boiled shrimp tropomyosins (TM) has never been reported. The aims of the study were to compare the stability of raw and boiled shrimp TM of Litopenaeus vanname...

  4. Evaluating a quantitative methionine requirement for juvenile Pacific white shrimp Litopenaeus vannamei

    Science.gov (United States)

    A 10-wk feeding trial was conducted as a third study (all conducted in our laboratory) to determine a quantitative requirement of juvenile Litopenaeus vannamei for sulfur amino acid methionine. Juvenile shrimp (mean weight 0.61 +/- 0.13 g) were reared in 110-L aquaria in a seawater recirculating sy...

  5. Uso tradicional de fauna silvestre por el grupo mayangna de Musawás en la Reserva de Biosfera Bosawas

    Directory of Open Access Journals (Sweden)

    Marlon José López González

    2001-08-01

    Full Text Available La presente investigación Se llevó a cabo en la comunidad de Musawás, municipio de Bonanza, Región Autónoma Atlántico Norte, en la Reserva Bosawas. Se realizó una descripción de los principales usos de la fauna silvestre por parte de los indígenas mayangnas. Se encontró que el 100% de los entrevistados hacen uso de la fauna silvestre para la alimentación, un 12% para artesanía, un 55% para el comercio, un 53% para medicina, un 20% para diversión y un gran porcentaje hace un uso mítico de la fauna silvestre. Esta gama de usos cobra una gran importancia en la preservación del recurso fauna. En los mitos se encontraron formas específicas de explicación y ordenamiento de los sucesos con la fauna silvestre; de esta manera responden y explican los fenómenos naturales y las actividades de sobrevivencia consideradas como sobrenaturales.

  6. Redescrição de Angiostrongylus (Parastrongylus costaricensis isolado de novo hospedeiro silvestre, Proechimys sp., na Venezuela (Metastrongyloidea, Angiostrongylidae

    Directory of Open Access Journals (Sweden)

    Cláudia Portes Santos

    1985-03-01

    Full Text Available Angiostrongylus (Parastrongylus costaricensis, nematódeo encontrado em artérias mesentéricas do rato silvestre Proechimys sp., na Venezuela, é redescrito e colocado no subgênero Parastorngylus.Angiostrongylus (Parastrongylus costaricensis is reported from a new host, Proechimys sp., a rodent collected in Venezuela. A study of the species is presented.

  7. Reproducción de cochinilla silvestre Dactylopius opuntiae (Homóptera: Dactylopiidae) Reproduction of wild cochineal Dactylopius opuntiae (Homoptera: Dactylopiidae)

    OpenAIRE

    Arnoldo Flores-Hernández; Bernardo Murillo-Amador; Edgar Omar Rueda-Puente; José Cruz Salazar-Torres; José Luis García-Hernández; Enrique Troyo-Diéguez

    2006-01-01

    Por sus características, la cochinilla silvestre ofrece perspectivas de aprovechamiento como fuente de carmín y para el mejoramiento genético de la grana fina. Por lo anterior, se estableció una multiplicación y crianza de cochinilla silvestre, proveniente de la zona árida del norte de México para caracterizarla, utilizando como hospedero a Opuntia megacantha Salm Dyck. Los resultados indican que la especie silvestre pertenece al género-especie Dactylopius opuntia. Se determinó la presencia d...

  8. Arbovírus Ilheus em aves silvestres (Sporophila caerulescens e Molothrus bonariensis

    Directory of Open Access Journals (Sweden)

    Pereira Luiz Eloy

    2001-01-01

    Full Text Available OBJETIVO: Relatar o isolamento do vírus Ilheus no Estado de São Paulo e avaliar o seu impacto para a saúde pública. MÉTODOS: O isolamento de vírus foi realizado em camundongos albinos Swiss, a partir de sangue de aves silvestres, capturadas com redes de espera tipo mist net, armadas no nível do solo, no Parque Ecológico do Tietê, São Paulo. A identificação das cepas isoladas foi feita pelos testes de inibição da hemaglutinação, fixação de complemento e neutralização em camundongos. Amostras de plasma de aves e de mamíferos silvestres foram submetidas à pesquisa sorológica para detecção de anticorpos inibidores de hemaglutinação. RESULTADOS: Foram isoladas duas cepas do vírus Ilheus em sangue de aves das espécies Sporophila caerulescens e Molothrus bonariensis e detectados anticorpos em aves das espécies Columbina talpacoti, Geopelia cuneata, Molothrus bonariensis e Sicalis flaveola, em sagüis das espécies Callithrix jacchus e Callithrix penicillata e no quati Nasua nasua. CONCLUSÕES: O isolamento do vírus Ilheus e a detecção de anticorpos específicos em aves residentes, migratórias e de cativeiro, em sagüis e quatis, comprovam a presença desse agente no Parque Ecológico do Tietê. O comportamento migratório de aves silvestres pode determinar a introdução do vírus em outras regiões. Considerando-se a patogenicidade para o homem e a confirmação da circulação desse agente viral em área urbana, freqüentada para atividade de lazer e de educação, o risco de ocorrência de infecção na população humana não pode ser descartado.

  9. Helmintos endoparasitos de vertebrados silvestres em Mato Grosso do Sul, Brasil

    Directory of Open Access Journals (Sweden)

    Luiz Eduardo Roland Tavares

    Full Text Available RESUMO Uma lista de espécies de vertebrados silvestres e as espécies de helmintos endoparasitos associadas foi organizada para o estado de Mato Grosso do Sul. Foram encontrados registros de 291 espécies de helmintos endoparasitos, sendo 9 de Acanthocephala, 226 de Nematoda e 65 espécies de Platyhelminthes (23 de Cestoda e 42 de Trematoda associadas a 20 espécies de peixes, nove de anfíbios, 17 de répteis, 103 de aves e 27 espécies de mamíferos. Apenas 12% dos vertebrados do estado foram estudados quanto a sua parasitofauna, ressaltando o quanto a biodiversidade de helmintos parasitos no estado ainda é subestimada.

  10. Orientalismos peninsulares en el levante andaluz. Nombres y usos de algunas plantas silvestres

    Directory of Open Access Journals (Sweden)

    Torres Montes, Francisco

    2000-06-01

    Full Text Available The author describes sorne wild plants from the provinces of Almería, Granada and Jaén (Andalusia, Spain that have folk names from Eastern Iberia. For each plant he provides the scientific and folk name, the isoglottic line and the lexical arca. He also informs about the use of these plants in folk and household medecine, religious rites, animal husbandry, etc.En este estudio se presentan algunas plantas silvestres de Almería, Granada y Jaén cuyos nombres vulgares proceden del oriente peninsular. De cada planta se da, junto al nombre vulgar y científico, la isoglosa o límite de los orientalismos y sus correspondientes áreas léxicas. Al mismo tiempo se hace el estudio etnográfico de sus usos en la medicina popular o doméstica, ritos religiosos, ganadería, etc.

  11. Deep-water shrimp fisheries in Latin America: a review Pesquerías de camarones de aguas profundas en América Latina: una revisión

    Directory of Open Access Journals (Sweden)

    Ingo S Wehrtmann

    2012-09-01

    á expandiendo sus actividades hacia aguas profundas. Las características del ciclo de vida de estos recursos de aguas profundas los hacen más vulnerables a la explotación que la mayoría de los recursos de aguas someras. Además, la falta de información sobre la ecología de la mayoría de las especies de aguas profundas constituye una limitación importante para el desarrollo e implementación de estrategias de manejo. Este escenario ha causado preocupación sobre la sustentabilidad de estos recursos y de los posibles impactos ambientales en los ecosistemas de aguas profundas. La pesca comercial en América Latina se extiende también hacia aguas profundas y, considerando las preocupaciones anteriormente mencionadas, se requiere la necesidad de compilar la información disponible sobre los recursos de camarones de aguas profundas y la situación actual de estas pesquerías en América Latina. Esta revisión se enfoca en México, Centro América, Perú, Chile y Brasil y describe las especies explotadas, la flota pesquera (siempre cuando sea disponible, las estadísticas pesqueras y las estrategias de manejo. Un total de 17 especies (10 spp. de Penaeoidea y 7 spp. de Caridea son de interés comercial en América Latina; sin embargo, camarones de aguas profundas solo se pescan actualmente en Costa Rica, Colombia y Chile. Un plan de manejo implementado existe en Chile y Colombia, mientras que en Brasil se aprobaron regulaciones sobre la pesca de camarones de la familia Aristeidae, pero éstas nunca han sido implementadas. Considerando la falta de información sobre la biología de los camarones de aguas profundas, lo que dificulta el desarrollo de estrategias adecuadas de manejo, se requiere urgentemente mejorar la comunicación y colaboración entre los diferentes actores en Latinoamérica. Se sugiere establecer un banco de datos abierto para búsquedas y actualizarlo constantemente, lo que servirá como fuente valiosa de información para investigadores y quienes deben tomar

  12. Population viability of Alouatta palliata (Primates: Atelidae) and Cebus capucinus (Primates: Cebidae) at Refugio de Vida Silvestre Privado Nogal, Sarapiquí, Heredia, Costa Rica

    National Research Council Canada - National Science Library

    Rodríguez-Matamoros, Jorge; Villalobos-Brenes, Federico; Gutiérrez-Espeleta, Gustavo A

    2012-01-01

    .... The aim of this work was to study the effects of population fragmentation on the long term viability of Alouatta palliata and Cebus capucinus populations, at Refugio de Vida Silvestre Privado Nogal, Sarapiquí (RVSPN), Heredia...

  13. Niveles de glucosa en primates de la especie Saimiri sciureus en cautiverio y en estado silvestre en el departamento del Caqueta

    National Research Council Canada - National Science Library

    Pacheco-Mora, Diego Fernando; Estrada-Cely, Gloria Elena; Triana-Mora, Agustin

    2010-01-01

    ... (mono ardilla o chichico), sometidos a cautiverio en el municipio de Florencia Caqueta, con los de primates en estado silvestre en la vereda Monterrey del municipio San Jose del Fragua--Caqueta...

  14. Uso tradicional de fauna silvestre por el grupo mayangna de Musawás en la Reserva de Biosfera Bosawas

    OpenAIRE

    Marlon José López González; Martín Lezama-López

    2001-01-01

    La presente investigación Se llevó a cabo en la comunidad de Musawás, municipio de Bonanza, Región Autónoma Atlántico Norte, en la Reserva Bosawas. Se realizó una descripción de los principales usos de la fauna silvestre por parte de los indígenas mayangnas. Se encontró que el 100% de los entrevistados hacen uso de la fauna silvestre para la alimentación, un 12% para artesanía, un 55% para el comercio, un 53% para medicina, un 20% para diversión y un gran porcentaje hace un uso mítico de la f...

  15. Evidences of abundant hemocyanin variants in shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Zhao, Xianliang; Guo, Lingling; Lu, Xin; Lu, Hui; Wang, Fan; Zhong, Mingqi; Chen, Jiehui; Zhang, Yueling

    2016-09-01

    Hemocyanin (HMC) is a multifunctional immune molecule present in mollusks and arthropods and functions as an important antigen non-specific immune protein. Our previous evidences demonstrated that Litopenaeus vannamei HMC might display extensive molecular diversities. In this study, bioinformatics analysis showed dozens of variant sequences of the HMC subunit with higher molecular weight from L. vannamei (LvHMC). Three variant fragments, named as LvHMCV1-3, which shared 85-99% nucleotide identity with that of the classical form of LvHMC (AJ250830.1), were cloned and characterized. Spatial expression profiles showed that LvHMCV1-3 had different tissue-specific distribution, which were affected by stimulation with six pathogenic bacteria, including Escherichia coli K12, Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio fluvialis, Streptococcus pyogenes and Staphylococcus aureus, with each variant fragment showing a specific stress pattern to different bacterial pathogens. Full length cDNA of LvHMCV3 was further cloned and characterized. The deduced amino acid sequence shared 92% identity with that of LvHMC, possessed a conserved structure characteristic of the HMC family and could be clustered into one branch along with other arthropod HMC in a phylogenetic tree. In addition, the recombinant protein of LvHMCV3 (rLvHMCV3) showed obvious agglutination activities against three aquaculture pathogenic bacteria including E. coli K12, V. parahaemolyticus and S.aureus at concentrations ranging from 31.25-62.5g/mL. It also showed obvious antibacterial activity against V. parahaemolyticus at concentrations 0.02-0.5mg/mL, and possessed the best inhibitive effects compared with those of rLvHMCV4 and rLvHMC. Co-injection of V. parahaemolyticus and rLvHMCV3 in L. vannamei showed significant decrease of the mortality rate at 24-72h after injection. Therefore, these studies suggested that L. vannamei had abundant HMC variants, which possessed obvious resistance to pathogenic

  16. Leishmaniose tegumentar alviericana na Ilha Grande, Rio de Janeiro: III. reservatórios silvestres e comensais

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    Nelson A. de Araújo Filho

    1981-12-01

    Full Text Available Em 203 animais silvestres e comensais examinados na Praia Vermelha, Ilha Grande, município de Angra dos fíeis, RJ, durante o estudo de um surto de Leishmaniose Tegumentar Americana, foram encontrados 2 exemplares de Proechimys dimidiatus, com lesões hipocrômicas nas extremidades das orelhas, e 1 exemplar de Rattus norvegicus norvegicus, com úlcera de dorso, cuja histopatologia revelou a presença de Leishmania sp. nos 3 exemplares.

  17. METODOLOGÍA BIOÉTICA PARA LA TOMA DE DECISIONES EN FAUNA SILVESTRE SOMETIDA A CAUTIVERIO

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    Gloria Elena Estrada-Cely

    2017-12-01

    Full Text Available El referente legal colombiano relacionado con la regulación del tráfico ilegal de fauna silvestre supone el establecimiento de estrategias de control que necesariamente generan una importante población de especímenes silvestres bajo custodia de las autoridades ambientales. Estos deben ser tratados de manera particular e individual, y la toma de decisiones en relación con ellos, desde el referente legal, supera el ámbito de lo clínico y etológico para ubicarse necesariamente en un ámbito bioético. Para el desarrollo de la investigación fueron empleados métodos analíticos y comparativos, a partir de la revisión del marco legal nacional en materia de uso de animales silvestres y su análisis bioético desde el cual se establecieron los lineamientos para el abordaje de casos, a partir de la construcción de una matriz bioética. Una vez desarrollado el proceso metodológico se estableció que el análisis de problemáticas que involucren especímenes de la fauna silvestre requiere de un abordaje que combine efectivamente referentes filosóficos, éticos, bioéticos, científicos y legales, procurando la resolución de cada caso dentro del marco de calidad de vida del animal y la protección de la vida humana y el ecosistema. Esta investigación propone una metodología efectiva para ello.

  18. PENDUGAAN NUTRIENT BUDGET TAMBAK INTENSIF UDANG, Litopenaeus vannamei

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    Rachmansyah Rachmansyah

    2016-11-01

    Full Text Available Penelitian ini bertujuan untuk mendapatkan nutrient budget tambak intensif udang Litopenaeus vannamei sebagai acuan alokasi input produksi pada tingkat kapasitas asimilasi lingkungan perairan. Pendugaan nutrient budget tambak udang intensif menggunakan pendekatan mass balance, dihitung berdasarkan input nutrien nitrogen - N dan fosfor – P yang berasal dari pakan, benih, pupuk, media probiotik, inflow, dan output nutrien yang ada pada produksi udang, outflow, dan endapan lumpur di dasar tambak. Sampel air, tanah, sedimen, plankton diambil sebelum penebaran dan setiap dua minggu selama pemeliharaan dari tiga petak tambak, masing-masing 5 titik sampel per petak tambak contoh. Analisis nitrogen dan fosfor dilakukan untuk sampel pakan, karkas udang awal dan akhir. Data managemen budi daya meliputi padat penebaran benur 50 ekor m-2, produksi 1.188—1.489 kg/0,25 ha, dan FCR 1,69—2,14; maka total input nutrien tambak udang Litopenaeus vannamei antara 171,9155—179,3778 (176 ± 3,9586 kgN dan 95,2533—99,4180(97,8340 ± 2,3348 kg P. Pakan mendominasi input N sebesar 61,96% ± 0,66%; disusul inflow 30,93% ± 0,70%; pupuk 6,52% ± 0,15%, serta media probiotik dan benur masing-masing <1%. Pola yang sama terjadi pada input phosphorous dengan komposisi 87,75% ± 0,24% dari pakan; 7,73% ± 0,19% pupuk; 4,05% ± 0,25% inflow dan media probiotik < 1%. Total output nitrogen tambak udang vannamei antara 107,1279-110,1438 (108,4957 ± 1,5274 kg N dan 51,6362—63,6576 (56,1292 ± 6,5604 kg P. Komposisi output nitrogen adalah outflow sebanyak 29,82% ± 3,20%; kemudian udang yang dipanen 21,32% ± 1,33%, lumpur atau sludge 10,40% ± 0,81%. Sedangkan komposisi output phosphorous didominasi oleh lumpur 39,03% ± 6,59%; kemudian udang yang dipanen 15,22% ± 0,85% dan outflow 3,09% ± 0,26%. Efisiensi pakan dan air melalui managemen budi daya yang benar menjadi peubah dominan penentu beban limbah tambak udang. This research was aimed to find out nutrient budget

  19. BUDIDAYA UDANG VANAME (Litopenaeus vannamei TEKNOLOGI INTENSIF MENGGUNAKAN BENIH TOKOLAN

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    Markus Mangampa

    2016-11-01

    Full Text Available Salah satu jenis udang yang cukup potensial untuk dikembangkan adalah udang vaname (Litopenaeus vannamei. Budidaya ini berkembang dengan teknologi intensif, namun terbatas pada golongan masyarakat menengah ke atas (padat modal. Riset ini bertujuan untuk mendapatkan data dan informasi pengaruh penggunaan tokolan terhadap produksi, Rasio Konversi Pakan (RKP pada pembesaran udang vaname teknologi intensif. Riset ini dilaksanakan di tambak Punaga, Takalar, Instalasi Balai Riset Perikanan Budidaya Air Payau (BRPBAP, menggunakan empat petak masing masing berukuran 4.000 m2/petak. Hewan uji adalah udang vaname dengan perlakuan: (A pembesaran dengan tebar benur (PL-12, dan (B pembesaran dengan tebar tokolan (PL-27. Setiap perlakuan dengan 2 ulangan, kepadatan benur dan tokolan adalah 50 ekor/m2, dan pemeliharaan berlangsung 80 hari di tambak. Hasil yang diperoleh pada perlakuan B memperlihatkan pertumbuhan mutlak (11,114±0,258 g/ekor, sintasan (92,55±0,23%, produksi (2.087,5±88,2 kg/petak lebih tinggi daripada perlakuan A yaitu: pertumbuhan mutlak (10,085±0,120 g/ekor, sintasan (90,83±8,51%, produksi (1.831,0±149,9 kg/petak, namun ketiga peubah ini berbeda tidak nyata antara kedua perlakuan. RKP lebih rendah pada perlakuan B (1,096±0,034 berbeda nyata dengan perlakuan A (1,257±0,048. Peubah kualitas air memperlihatkan sebaran kisaran yang merata untuk kedua perlakuan, kecuali nitrit (NO2 memperlihatkan kisaran yang tinggi pada perlakuan B (0,18235 mg/L dibandingkan dengan perlakuan A (0,0328 mg/L pada akhir penelitian. Hal ini disebabkan waktu panen yang berbeda sesuai dengan kondisi musim yaitu kualitas air sumber semakin menurun. Kualitas air sumber yang menurun ini diikuti oleh meningkatnya total vibrio di air laut mencapai; 4,33104 cfu/mL dibandingkan dalam air tambak 829.102 cfu/mL. Kesimpulan memperlihatkan bahwa penggunaan tokolan (PL-27 menghasilkan produksi yang tinggi dan rasio konversi pakan yang rendah. One species of shrimps that

  20. Mating systems in caridean shrimp (Decapoda: Caridea and their evolutionary consequences for sexual dimorphism and reproductive biology Sistemas de apareamiento en camarones carideos (Decapoda: Caridea y sus consecuencias evolutivas en el dimorfismo sexual y biología reproductiva

    Directory of Open Access Journals (Sweden)

    CRISTIÁN CORREA

    2003-06-01

    species with search & attend the situation is variable, and only some males benefit by acquiring large size while others do better by searching or changing sex. In contrast to other crustaceans free-living shrimp do not exhibit precopulatory mate guarding and they are relatively unaggressive. The characteristic and efficient escape behavior of shrimp using their contractile abdomen might be one important trait that has constrained the evolution of such behaviors. Our review indicates that the mating systems of caridean shrimp depend both on environmental and on biological characteristicsEn este articulo revisamos relaciones funcionales y evolutivas entre los sistemas de apareamiento de camarones carideos, y características específicas tales como biología/ecología general, sistemas sexuales, conducta y morfología. Basados en reportes bibliográficos se describió cuatro sistemas de apareamiento, y un quinto fue reconocido pero la informaci��n disponible fue insuficiente para describirlo en detalle. `Monogamia' ocurre en muchas especies que habitan en refugios monopolizables o huéspedes, especialmente cuando las condiciones ambientales restringen la probabilidad de encuentros entre conespecíficos. En contraste, las especies de vida libre experimentan encuentros más frecuentes y los machos pueden dominar o buscar: En el sistema de apareamiento `dominancia vecinal' los machos más grandes rinden mayor éxito reproductivo porque son los que tienen el mejor desempeño en la competencia agresiva por las hembras receptivas. En el sistema `pura búsqueda' son los machos pequeños los que lo hacen mejor porque su agilidad les permite buscar pareja eficazmente entre la población. El cuarto sistema de apareamiento es `búsqueda & compañía' que ocurre en simbiontes solitarios que experimentan condiciones demográficas y ecológicas variables: dependiendo de las condiciones ambientales y del estado ontogenético, puede ser conveniente para los machos buscar o permanecer

  1. Parasitos de aves e mamíferos silvestres em cativeiro no estado de Pernambuco

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    Pauline Marie de Souza Santos

    2015-09-01

    Full Text Available Resumo: Os animais silvestres são hospedeiros de uma grande variedade de parasitos que podem interferir em sua conservação ex situ. O objetivo deste estudo foi identificar os parasitos gastrointestinais (PGI e ectoparasitos dos animais do Centro de Triagem de Animais Silvestres (CETAS do Instituto Brasileiro de Meio Ambiente e Recursos Naturais Renováveis (IBAMA de Recife, Pernambuco, além de determinar os aspectos do manejo em cativeiro que possam estar relacionados com os parasitos identificados. Foram coletados ectoparasitos e amostras fecais de 223 aves e mamíferos, as quais foram processadas pelos métodos: microscopia direta, flutuação e sedimentação. Helmintos e/ou protozoários foram detectados em 91 (40,8% amostras fecais, sendo 64 (70,3% de aves e 27 (29,7% de mamíferos. Ovos de Capillaria sp., Ascaridida, Spirurida e oocistos de Eimeria sp. foram detectados nas amostras fecais das aves, enquanto ovos de Trichuris trichiura, Strongyloides sp., Toxocara canis, Ancylostoma sp., Strongylida e oocistos de Coccídios foram encontrados nas amostras fecais de mamíferos. Os ectoparasitos identificados em aves foram Colpocephalum turbinatum, Kurodaia (Kurodaia fulvofasciata, Halipeurus sp., Naubates sp., Saemundssonia sp., Austromenopon sp., Paragoniocotes sp., Brueelia sp., Myrsidea sp. and Pseudolynchia sp., enquanto em mamíferos os ectoparasitos identificados foram Rhipicephalus sanguineus, Amblyomma varium, A. calcaratum, A. nodosum, Ornithodoros talaje e Ctenocephalides felis felis. A. calcaratum e O. talaje são registrados pela primeira vez em Pernambuco e T. tetradactyla é apresentado como novo hospedeiro de O. talaje. Nenhum dos animais estudados apresentou sinais clínicos em decorrência da infecção/infestação parasitária. Parasitos com potencial zoonótico como T. trichiura, Strongyloides sp., T. canis e Ancylostoma sp. foram identificados em primatas não humanos e carnívoros. Precárias condições estruturais

  2. Epidemiology and control of wild rabies in Italy / Epidemiologia e profilassi della rabbia silvestre in Italia

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    Armando Giovannini

    1991-07-01

    Full Text Available Abstract The authors analyse biological characteristics of the fox Vulpes vulpes with regard to the epidemiology of sylvatic rabies in Italy. Emphasis is put on the lack of deepen studies both on fox population densities, and on important behavioural habits. A retrospective analysis of wild rabies epidemics in Italy from 1977 to 1988 has shown: 1 the spread of rabies by 20 to 80 km/year; 2 the existence of three/four year oscillations, clearly evident in single epidemics, but masked in the total number of cases in Italy; 3 the mosaic-like pattern of the final phase of the epidemic; 4 the extinction of outbreaks following the mosaic phase, in a few cases not correlated with human intervention. The authors criticize the control measures enforced in Italy, on the bases of the inefficacy and inefficiency of the thinning out programme and, on the contrary, the efficiency of the oral vaccination of the fox population. Riassunto Gli autori analizzano la biologia della Volpe (Vulpes vulpes in relazione all'epidemiologia della rabbia silvestre in Italia; sottolineano la grave carenza di dati nel nostro Paese sia sulla densità di popolazione della Volpe, sia su aspetti di ecologia comportamentale importanti per l'epidemiologia della rabbia silvestre in Italia dal 1977 al 1988 rilevando: 1 una velocità di avanzamento variabile da 20 a 80 km/anno; 2 una ciclicità tri-quadriennale per singola epidemia che può essere mascherata se si considera erroneamente la sommatoria dei casi accertati; 3 l'andamento a mosaico delle fasi finali delle epidemie; 4 l'estinzione dei focolai successivamente alla fase a mosaico, in alcuni casi non correlabile all'intervento dell'uomo. Infine, gli autori analizzano in modo critico la profilassi della rabbia nella Volpe attuata in Italia, rilevando la scarsa efficacia ed inefficienza dello sfoltimento e, in contrapposizione, l'efficacia e l'economicità della vaccinazione

  3. Isoenzymatic variability in wild potatoes Variabilidade isoenzimática em batata silvestre

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    Beatriz Helena Gomes Rocha

    2001-05-01

    Full Text Available Two species of wild potato Solanum commersonii, subspecies commersonii and malmeanum, and S. chacoense, subspecies muelleri occur in southern Brazil. Their rusticity and easy adaptation to extreme climatic conditions make them valuable for breeding programs. The objective of this work was to analyze the isoenzymatic variability of 113 clones of wild potato subspecies. They were collected and maintained at Embrapa-Centro de Pesquisa Agropecuária de Clima Temperado, at Pelotas, RS, Brazil. Enzymes involved in energetic (group I or in peripherical (group II metabolism constituted the material used. Polyacrylamide horizontal gel electrophoresis was used to analyze peroxidase, aspartate transaminase, phosphoglucomutase and isocitrate dehydrogenase isoenzymes. Solanum spp. has considerable genetic variability for isoenzymatic patterns. Cluster analysis classified the clones into 51 subgroups, based on electrophoretic variants of group I enzymes, and into 89, when group II enzyme variants were added. Genotypic differentiation of S. chacoense muelleri in relation to S. commersonii commersonii and S. commersonii malmeanum is evident when expressed through similarity and cluster analysis.No sul do Brasil ocorrem apenas duas espécies silvestres de batata, Solanum commersonii, com as subespécies commersonii e malmeanum, e S. chacoense, com a subespécie muelleri, de interesse aos programas de melhoramento, pela rusticidade e fácil adaptação a condições climáticas extremas. O objetivo deste trabalho foi analisar a variabilidade isoenzimática de 113 clones de batata silvestre. O material foi coletado e mantido na Embrapa-Centro de Pesquisa Agropecuária de Clima Temperado, em Pelotas, RS. Foram usadas enzimas envolvidas nos metabolismos energético (grupo I e periférico (grupo II. Eletroforese horizontal em gel de poliacrilamida foi empregada para análise de isoenzimas de peroxidase, aspartato transaminase, fosfoglucomutase e isocitrato

  4. Caracterización morfológica de accesiones silvestres de guayaba

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    Muñoz florez Jaime Eduardo

    2009-06-01

    Full Text Available Se realizó la caracterización morfológica de 22 accesiones silvestres de guayaba Psidium guajava L. 14 colectadas en el municipio de Restrepo (Valle del Cauca, seis en Armenia (Quindío y dos en Pereira (Risaralda. Se utilizaron 12 descriptores cuantitativos y 10 cualitativos de tallo, hojas y frutos; el análisis de agrupamiento se hizo mediante el coeficiente de Dice-Nei-Li y el promedio aritmético no ponderado (UPGMA. La mayor variabilidad se halló en los descriptores peso de la pulpa (CV = 55.92%, peso (CV = 45.23%, y acidez del fruto (CV = 44.75%. El análisis de agrupamientos con base en caracteres cuantitativos permitió establecer cuatro grupos: las accesiones del grupo A (Armenia, presentaron valores promedio de los descriptores de la calidad del fruto (grados Brix, acidez del fruto y relación grados Brix/acidez y valores altos de contenido de pulpa. La mayoría de accesiones del grupo C (Restrepo tuvieron altos valores de calidad del fruto y bajo contenido de pulpa. El grupo B, constituido por accesiones de Armenia y Pereira, se diferenció por valores bajos en los descriptores de rendimiento del fruto (peso del fruto, de la pulpa y diámetro de la cavidad seminal. Las accesiones del grupo D (Restrepo mostraron valores promedios en calidad y rendimiento del fruto. Los descriptores cuantitativos se reunieron en tres variables sintéticas para rendimiento y calidad del fruto que representaron 76.86% de la variabilidad total.

  5. Caracterización morfológica de accesiones silvestres de guayaba

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    Liliana Jiménez Lozano

    2009-04-01

    Full Text Available Se realizó la caracterización morfológica de 22 accesiones silvestres de guayaba Psidium guajava L. 14 colectadas en el municipio de Restrepo (Valle del Cauca, seis en Armenia (Quindío y dos en Pereira (Risaralda. Se utilizaron 12 descriptores cuantitativos y 10 cualitativos de tallo, hojas y frutos; el análisis de agrupamiento se hizo mediante el coeficiente de Dice-Nei-Li y el promedio aritmético no ponderado (UPGMA. La mayor variabilidad se halló en los descriptores peso de la pulpa (CV = 55.92%, peso (CV = 45.23%, y acidez del fruto (CV = 44.75%. El análisis de agrupamientos con base en caracteres cuantitativos permitió establecer cuatro grupos: las accesiones del grupo A (Armenia, presentaron valores promedio de los descriptores de la calidad del fruto (grados Brix, acidez del fruto y relación grados Brix/acidez y valores altos de contenido de pulpa. La mayoría de accesiones del grupo C (Restrepo tuvieron altos valores de calidad del fruto y bajo contenido de pulpa. El grupo B, constituido por accesiones de Armenia y Pereira, se diferenció por valores bajos en los descriptores de rendimiento del fruto (peso del fruto, de la pulpa y diámetro de la cavidad seminal. Las accesiones del grupo D (Restrepo mostraron valores promedios en calidad y rendimiento del fruto. Los descriptores cuantitativos se reunieron en tres variables sintéticas para rendimiento y calidad del fruto que representaron 76.86% de la variabilidad total.

  6. Augusto Boal en la educación social: del teatro del oprimido al psicodrama silvestre

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    Manuel F. Vieites García

    2015-02-01

    Full Text Available La obra de Augusto Boal Teatro del Oprimido se suele situar entre las aportaciones más transcendentales del teatro del siglo XX, en tanto sus dictados teóricos y sus propuestas metodológicas y prácticas han calado en ámbitos de la educación social y teatral relacionados con el desarrollo de la democracia cultural. Pasados cuarenta años desde sus primeras experiencias, en este trabajo se propone una genealogía del teatro del oprimido a partir de una lectura crítica de sus textos fundacionales y de alguna experiencia considerada paradigmática en su desarrollo, para mostrar su naturaleza, aportes, fortalezas y debilidades. Igualmente se muestra el tránsito entre una dimensión más educativa y teatral y otra más centrada en la terapia y el desarrollo personal, para concluir que las técnicas y recursos empleados en teatro del oprimido, que proceden de fuentes muy diversas, siguen teniendo potencialidades indudables en propuestas de intervención social, cultural y educativa propias de la pedagogía teatral y la pedagogía social.Cómo referenciar este artículoVieites García, M. F. (2015. Augusto Boal en la educación social: del teatro del oprimido al psicodrama silvestre. Foro de Educación, 13(18, pp. 161-179. doi: http://dx.doi.org/10.14516/fde.2015.013.018.009 

  7. Diagnóstico da fauna silvestre em empresas florestais brasileiras Diagnosis of wild fauna in brazilian forest companies

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    Giovanna Debortoli Medeiros

    2009-02-01

    Full Text Available Com o objetivo de conhecer a situação atual da fauna silvestre em empresas florestais brasileiras, foram pesquisadas 42 razões sociais, entre Associadas e Co-Participantes da Sociedade de Investigações Florestais (SIF, as quais possuem plantios florestais próprios. As informações foram obtidas com base em questionário enviado às referidas empresas, via correio eletrônico, sendo as respostas obtidas também por esse mecanismo. Entre os vários resultados, destaca-se o fato de que 90,9% das empresas associadas já realizaram levantamentos qualitativos da fauna silvestre. No entanto, de modo geral há notória carência de infra-estrutura nas empresas pesquisadas para atender a trabalhos específicos de manejo e conservação da fauna silvestre.Forty-two companies, associates and co-participants of the Forest Investigation Society (SIF and owners of private forest plantations, were assessed to determine the current situation of wild fauna in Brazilian forest companies. Information was obtained through a questionnaire sent to and received from the companies by e-mail. One of the several results obtained was that 90.9% of the associate companies had already carried out qualitative surveys on wild fauna stands.. On the other hand, in general, there is a total lack of infrastructure in the surveyed companies, to specifically assist in the management and conservation of wild fauna.

  8. Tráfico y tenencia ilegal de fauna silvestre en el departamento de Boyacá

    OpenAIRE

    Alejandro Cabrejo Bello

    2010-01-01

    Con el fin de establecer cuáles son los principales grupos taxonómicos, suparticipación y las ciudades con mayores índices de tráfico y tenencia ilegal de vida silvestre en el departamento de Boyacá, jurisdicción de la Corporación Autónoma Regional de Boyacá- Corpoboyacá, se tomaron reportes de la base de datos de dicha entidad entre los años 2004 y 2008. Se hallaron registros de 398 individuos decomisados por parte de la autoridad ambiental y otros por entregas voluntarias. El grupo con mayo...

  9. Parámetros hematológicos en polluelos de psitácidos en cautiverio de origen silvestre

    OpenAIRE

    J.A Herrera; A. Ávalos; Herrera, G.; D. Gómez; Varela, A.; Guzmán, A.; Rosales, A. M.

    2013-01-01

    El objetivo de este estudio fue describir los parámetros hematológicos en 69 polluelos en cautiverio de origen silvestre, clínicamente sanos, menores de tres meses de edad, en cuatro especies de psitácidos: Pionus senilis (n = 18), Amazona albifrons (n = 15), Amazona autumnalis (n = 25) y Amazona oratrix (n = 11), procedentes de la región central de México. Para determinar dichos parámetros, de cada ejemplar se obtuvieron 500 μl de sangre por punción de la vena braquial. En las c...

  10. Enraizamento de espécies silvestres de maracujazeiro utilizando cinco doses de ácido indolilbutírico

    OpenAIRE

    Vaz, Carolina de Faria; Peixoto, José Ricardo; Junqueira,Nilton Tadeu Vilela; Braga,Marcelo Fideles; Santos,Erivanda Carvalho dos; Fonseca,Kenia Gracielle da; Junqueira,Keize Pereira

    2009-01-01

    Este trabalho teve como objetivo avaliar em casa de vegetação o potencial de enraizamento de diferentes espécies silvestres de maracujá, utilizando diferentes doses de ácido indolilbutírico. Estacas herbáceas de plantas adultas de Passiflora setacea, P. coccinea, P. amethystina, P. edulis, P. edulis x P. setacea e P. coccinea x P. setacea foram coletadas e tratadas com doses de 0; 250; 500; 750 e 1.000 mgL-1 de IBA, sendo em seguida plantadas em bandejas com substrato umedecido e mantidas sob...

  11. Diagnóstico jurídico sobre el decomiso y manejo postdecomiso de fauna silvestre en el departamento del Chocó

    OpenAIRE

    Hinestroza Cuesta, Lisneider; Mena Valencia, Marisela; Palacios Sánchez, Kelly Johana; 2011-12-31

    2011-01-01

    En este artículo se presenta un estudio de carácter descriptivo sobre los decomisos y el manejo postdecomiso de fauna silvestre en el departamento del Chocó utilizado como corredor para el tráfico y comercialización ilegal; se analizan, a partir del marco jurídico colombiano, la definición de fauna silvestre, decomiso y sus consecuencias jurídicas. Entre el 2005 y marzo del 2011 se realizaron en el Chocó 904 decomisos: 316 aves, 321 reptiles y 264 de mamíferos, 1 anfibio, 1 arácnido y 1 pez. ...

  12. Priorización de enfermedades virales zoonóticas en la interfaz de cerdos silvestres, cerdos domésticos y seres humanos

    OpenAIRE

    Diana Benavides-Arias; Diego Soler-Tovar

    2016-01-01

    Introducción. Para entender la ecología de las enfermedades es necesario comprender los agentes patógenos en la interfaz de vida silvestre y ganado. Los cerdos silvestres (Sus scrofa) constituyen un problema sanitario cuando se trata de prevenir y controlar las enfermedades zoonóticas, pues en ocasiones sus poblaciones son portadores de agentes infecciosos transmisibles a los cerdos domésticos y a otras especies animales, incluidos los seres humanos. Objetivo. Priorizar las enfermedades zo...

  13. Análise dos perigos associados ao camarão Litopenaeus vannamei no Brasil

    OpenAIRE

    Marcela Leite do Nascimento

    2013-01-01

    O objetivo deste trabalho foi identificar na cadeia produtiva do camarão Litopenaeus vannamei os perigos à saúde animal e à saúde pública e as respectivas medidas de controle, a partir de levantamentos bibliográficos. Os principais perigos à saúde animal identificados foram os vírus da Mancha Branca, Cauda Amarela, Mionecrose Infecciosa, síndrome da Taura e bactérias hepatonecrosantes dos camarões, que ocasionam grandes prejuízos para a cadeia produtiva, devido à queda da produtividade e às e...

  14. Direct nitrous oxide emission from the aquacultured Pacific white shrimp (Litopenaeus vannamei)

    OpenAIRE

    Heisterkamp, Ines M; Schramm, Andreas; de Beer, Dirk; Stief, Peter

    2016-01-01

    The Pacific white shrimp (Litopenaeus vannamei) is widely used in aquaculture, where it is reared at high stocking densities, temperatures, and nutrient concentrations. Here we report that adult L. vannamei shrimp emit the greenhouse gas nitrous oxide (N2O) at an average rate of 4.3 nmol N2O/individual × h, which is 1 to 2 orders of magnitude higher than previously measured N2O emission rates for free-living aquatic invertebrates. Dissection, incubation, and inhibitor experiments with specime...

  15. A Comparative study on the nonspecific immunity of juvenile Litopenaeus vannamei ever inhabiting freshwater and seawater

    Science.gov (United States)

    Jia, Xuying; Ding, Sen; Wang, Fang; Dong, Shuanglin

    2014-06-01

    A study on the nonspecific immunity of Litopenaeus vannamei ever inhabiting freshwater and seawater was carried out at different molt stages by comparing their total hemocyte count (THC) and respiratory burst (RB) and activity of phenol oxidase (PO), nitric oxide synthase (NOS) and lysozyme (LY). Two-way ANOVA showed that salinity and molt stage independently affected THC and RB and the activity of PO, NOS and LY of juvenile L. vannamei significantly ( P vannamei were significantly lower in freshwater than in seawater; whereas THC was significantly higher in freshwater than in seawater ( P vannamei was cultured in freshwater.

  16. Influence of probiotics on the growth and digestive enzyme activity of white Pacific shrimp ( Litopenaeus vannamei)

    Science.gov (United States)

    Gómez, R. Geovanny D.; Shen, M. A.

    2008-05-01

    The influence of Bacillus probiotics on the digestive enzyme activity and the growth of Litopenaeus vannamei were determined in this study. The shrimp was treated with five percentages (1.5, 3.0, 4.5, 6.0 and 7.5) of probiotics ( Bacillus spp.) supplemented to the feed and cultured for 45d. The growth measured as the weight gain at the end of culturing was significantly ( Pprobiotic-treated shrimps than that of the control (without receiving probiotics). Activities of protease and amylase, two digestive enzymes of the midgut gland and the intestine were significantly ( Pprobiotic-treated shrimp than in the control.

  17. Sperm quality of Litopenaeus vannamei broostock injected by PMSG and antidopamin

    Directory of Open Access Journals (Sweden)

    Fahmi Akbar

    2015-10-01

    Full Text Available ABSTRACT The important role in determining the productivity of shrimp was the quality and quantity of shrimp sperm. The decreasing of hatching rate was predicted as the effect of the decreasing quality of sperm. It then could  influence  the number and quality of naupli produced. Hormonal induction of maturation is one of alternative solution that can improve shrimp sperm quality. This study was conducted to examine the effect of pregnant mare serum gonadotropin (PMSG and antidopamine (AD injection on white shrimp Litopenaeus vannamei sperm quality. This research consisted of six treatments which were treatment without eyestalk ablation, eyestalk ablation, and premix PMSG hormone, and AD at the dose  of 0.1 mL/kg, 0.25 mL/kg, 0.5 mL/kg, and 1 mL/kg. The observed parameters were sperm count and percentage of normal and abnormal sperm. The results showed that PMSG hormone and AD injection could improve sperm quality of L. vannamei shrimp. Hormone at the dose of 0.25 mL/kg and 0.5 mL/kg were the optimal doses to increase sperm count and the percentage of normal sperm, also to lower the percentage of abnormal sperm. Keyword: PMSG, AD, sperm quality, Litopenaeus vannamei  ABSTRAK Kuantitas dan kualitas sperma udang jantan sangat berperan penting dalam menentukan produktivitas udang. Terjadinya penurunan daya tetas telur udang diduga karena terjadinya penurunan kualitas sperma. Hal ini berpengaruh terhadap jumlah dan kualitas nauplius yang diproduksi. Induksi maturasi secara hormonal merupakan salah satu alternatif yang dapat meningkatkan kualitas sperma udang. Penelitian ini dilakukan untuk mengkaji pengaruh penyuntikan pregnant mare serum gonadotropin (PMSG dan antidopamin (AD terhadap kualitas sperma udang vaname Litopenaeus vannamei. Penelitian terdiri atas enam perlakuan, yaitu perlakuan tanpa ablasi mata, ablasi mata, dan injeksi dengan premix hormon PMSG dan AD dosis 0,1 mL/kg, 0,25 mL/kg, 0,5 mL/kg, dan 1 mL/kg. Parameter yang diamati

  18. Comparison of immune response of Pacific white shrimp, Litopenaeus vannamei, after multiple and single infections with WSSV and Vibrio anguillarum

    Science.gov (United States)

    Our previous study demonstrated that Pacific white shrimp (Litopenaeus vannamei) infected by multiple pathogens showed higher mortality and death occurred more quickly than those infected by a single pathogen [1]. For better understanding the defense mechanism against white spot syndrome virus (WSSV...

  19. Replacement of fish meal with black soldier fly meal in practical diets for Pacific whiteleg shrimp Litopenaeus vannamei

    Science.gov (United States)

    Shrimp account for 15 percent of the total value of internationally traded fishery products, and currently are the largest single aquaculture commodity in value terms. The Pacific whiteleg shrimp (Litopenaeus vannamei) generated USD 11 billion from a production volume of 2.7 million metric tons (mm...

  20. A major SNP resource for dissection of phenotypic and genetic variation in Pacific white shrimp (Litopenaeus vannamei)

    NARCIS (Netherlands)

    Ciobanu, D.C.; Bastiaansen, J.W.M.; Magrin, J.; Rocha, J.L.; Jiang, D.H.; Yu, N.; Geiger, B.; Deeb, N.; Rocha, D.; Gong, H.; Kinghorn, B.P.; Plastow, G.S.; Steen, van der H.A.M.; Mileham, A.J.

    2010-01-01

    Bioinformatics and re-sequencing approaches were used for the discovery of sequence polymorphisms in Litopenaeus vannamei. A total of 1221 putative single nucleotide polymorphisms (SNPs) were identified in a pool of individuals from various commercial populations. A set of 211 SNPs were selected for

  1. Tratamiento de efluentes del cultivo de Litopenaeus vannamei por sedimentación y filtración por la ostra Crassostrea rhizophorae

    National Research Council Canada - National Science Library

    Ramos, Roberto; Vinatea, Luis; Costa, Rejane da

    2008-01-01

    A escala de laboratorio se evaluaron las eficiencias de remoción del material particulado y nutrientes disueltos desde efluentes de cultivo de Litopenaeus vannamei, utilizando un sistema combinado de dos fases, sedimentación...

  2. DIAGNÓSTICO JURÍDICO SOBRE EL DECOMISO Y MANEJO POSTDECOMISO DE FAUNA SILVESTRE EN EL DEPARTAMENTO DEL CHOCÓ

    Directory of Open Access Journals (Sweden)

    Lisneider Hinestroza Cuesta

    2011-12-01

    Full Text Available En este artículo se presenta un estudio de carácter descriptivo sobre los decomisos y el manejo postdecomiso de fauna silvestre en el departamento del Chocó utilizado como corredor para el tráfico y comercialización ilegal; se analizan, a partir del marco jurídico colombiano, la definición de fauna silvestre, decomiso y sus consecuencias jurídicas. Entre el 2005 y marzo del 2011 se realizaron en el Chocó 904 decomisos: 316 aves, 321 reptiles y 264 de mamíferos, 1 anfibio, 1 arácnido y 1 pez. La tendencia ha sido el aumento. La principal opción de disposición de fauna decomisada es la Liberación. Se han impuesto solamente sanciones de tipo administrativo sin reporte en el Registro Único de Infractores Ambientales y no se ha adelantado ninguna investigación de tipo penal

  3. Species identification of white shrimps Litopenaeus vannamei and other Penaeus sp. by using PCR-RFLP of Cytochrome Oxidase Subunit I (COI) gene

    OpenAIRE

    Chotigeat, W.; Chanumpai, A.; Wanna, W.; Hualkasin, W.; Phongdara, A.

    2004-01-01

    The morphology of imported white shrimp (Litopenaeus vannamei) is similar to other native white shrimp of the Thai peninsula (Penaeus merguiensis, Penaeus silasi and Penaeus indicus) and could not be separated clearly in the juvenile stage. It causes the mixture of them in the culture pond leading to problems on the farm. This work identified the different pattern of cytochrome oxidase subunit I (COI) gene in mitochondrial DNA (mtDNA) of 7 species (Litopenaeus vannamei, Penaeus merguiensis, P...

  4. Culturable fungal diversity of shrimp Litopenaeus vannamei boone from breeding farms in Brazil

    Directory of Open Access Journals (Sweden)

    Lidiane Roberta Cruz da Silva

    2011-03-01

    Full Text Available Litopenaeus vannamei, which is the most common shrimp species cultivated in the northeast of Brazil, is very susceptible to microbial diseases, and this consequently affects productivity. There are reports of bacteria, viruses and protozoa in these shrimp, but not fungi. This study aims to isolate and identify fungi present in shrimp Litopenaeus vannamei, and in their nursery waters, at two breeding farms in Brazil. The pathogenic potential of the isolates was assessed through the qualitative detection of proteases and aflatoxin B production. The 146 isolated fungi comprised 46 species. Aspergillus, Penicillium and Furarium were the three most relevant genera and Aspergillus flavus was the predominant species with a total of 33 isolates. Most of the isolated species are known as potentially pathogenic to humans and other animals. Eighteen isolates of A. flavus and two of A. parasiticus were able to produce aflatoxin B and 33 out of the 46 species produced protease, indicating that these fungi may also become pathogenic to shrimp and their consumers.

  5. Bacterial communities associated with white shrimp (Litopenaeus vannamei larvae at early developmental stages

    Directory of Open Access Journals (Sweden)

    ANTONIUS SUWANTO

    2010-04-01

    Full Text Available Bacterial communities associated with white shrimp (Litopenaeus vannamei larvae at early developmental stages. Biodiversitas 11 (2: 65-68.Terminal Restriction Fragment Length Polymorphism (T-RFLP was used to monitor the dynamics of the bacterial communities associated with early developmental stages of white shrimp (Litopenaeus vannamei larvae. Samples for analysis were egg, hatching nauplii, 24 hours old nauplii, and 48 hours old nauplii which were collected from one cycle of production at commercial hatchery. T-RFLP results indicated that the bacterial community associated with early stages of shrimp development might be transferred vertically from broodstock via egg. There was no significant difference between bacterial communities investigated, except the bacterial community of 48 hours old nauplii. Diversity analyses showed that the bacterial community of egg had the highest diversity and evenness, meanwhile the bacterial community of 48 hours old nauplii had the lowest diversity. Nine phylotypes were found at all stages with high abundance. Those TRFs were identified as γ- proteobacteria, α-proteobacteria, and bacteroidetes group.

  6. Effects of cultured shrimp (Litopenaeus vannamei consumption on serum lipoproteins of healthy normolipidemic men

    Directory of Open Access Journals (Sweden)

    Farzaneh Yousefi

    2012-12-01

    Full Text Available Background: It has been suggested that moderate shrimp consumption in normolipidemic subjects will not adversely affect the overall lipoprotein profile. Hence, shrimp consumption can be included in “healthy heart" nutritional guidelines. However, the effects of cultured shrimp on serum lipoproteins of normal subjects have not yet investigated. Material and Methods: Twenty-five healthy normolipidemic men who were workers of a shrimp farm in Bushehr province participated in a quasi-experimental study. In a crossover six weeks trial, the effect of three days per week diet (containing 300 g cultured shrimp Litopenaeus vannamei /day on serum lipid profile was compared with a zero-marine baseline diet. Results: After six weeks trial, serum triglyceride and HDL-cholesterol levels were not significantly changed from the baseline levels (p>0.05. However, total cholesterol and LDL-cholesterol levels, total cholesterol to HDL-cholesterol and LDL-cholesterol to HDL-cholesterol ratios were significantly increased (p<0.0001. Conclusion: Moderate cultured shrimp (Litopenaeus vannamei consumption can increase total cholesterol and LDL-cholesterol levels in normolipidemic men. Although a diet containing native shrimp has many benefits for healthy persons, but we do not recommend cultured shrimp in a healthy heart diet for persons with dyslipidemia or cardiovascular diseases.

  7. Sensitivity of Larvae and Adult and the Immunologic Characteristics of Litopenaeus vannamei under the Acute Hypoxia

    Directory of Open Access Journals (Sweden)

    Hailong Zhou

    2014-01-01

    Full Text Available Litopenaeus vannamei is one of the most commercially important species of shrimp in the world. In this study, we performed acute hypoxia tests with Litopenaeus vannamei to estimate 12 h median lethal concentration (LC50 values at different life stages. The results indicated that the 12 h LC50 values were significantly different in different life stages of shrimp (P<0.05. The maximum value of 12 h LC50 was 2.113 mg L−1 for mysis III, and the minimum value was 0.535 mg L−1 for adult shrimp with an average total length of 6 cm. The study also determined the hemocyanin concentration (HC and the total hemocyte counts (THC in the conditions of hypoxia and reoxygenation. These results showed that the THC decreased and the HC increased under hypoxia, and the THC increased and the HC decreased in the condition of reoxygenation. These results can provide fundamental information for shrimp farming and seedling and also can guide the breeding selection, as well as being very helpful to better understand the hypoxia stress mechanism of shrimp.

  8. Riesgo de transmisión de la enfermedad de Chagas por intrusión de triatominos y mamíferos silvestres en Bucaramanga, Santander, Colombia

    Directory of Open Access Journals (Sweden)

    Marlene Reyes

    2017-01-01

    Conclusiones. El hallazgo de especies de triatominos intrusivas y de mamíferos con T. cruzi en el domicilio y el peridomicilio, así como en los bosques periurbanos, demuestra el riesgo de infección en las poblaciones que habitan en viviendas urbanas adyacentes a los ecótopos donde se mantiene el ciclo silvestre.

  9. Los Hongos Comestibles Silvestres De Santa Catarina Del Monte, Estado De México

    Directory of Open Access Journals (Sweden)

    B. Arteaga Martínez

    2006-01-01

    Full Text Available Se realizó un estudio sobre la producción de hongos comestibles silvestres en el bosque de coníferas de Santa Catarina del Monte, Estado de México. La fase experimental se llevó a cabo en cinco parcelas de 1 ha cada una, ubicadas en un bosque de pino (Pinus hartwegii Lindl. y en un bosque de oyamel (Abies religiosa (Schl. et Cham. H.B.K.. Se determinó la fenología de aparición de las especies fúngicas, la producción por unidad de superficie, el valor económico por hectárea y las condiciones ecológicas del bosque, donde se desarrollan los hongos. La producción media mensual para cada tipo de vegetación se analizó en función de la variación de la temperatura, precipitación y humedad relativa durante el año. Por el método de correlación se determinó el grado de asociación entre la producción de hongos y las variables: número de especies de hongos, cobertura arbórea, edad promedio del arbolado, diámetro promedio del arbolado, temperatura media mensual y precipitación. Siguiendo el procedimiento del análisis de regresión múltiple y utilizando el método de Stepwise, se identificó qué características ecológicas se relacionan con la producción de hongos, resultando que la temperatura media mensual, diámetro promedio del arbolado, precipitación mensual y edad promedio del arbolado fueron las variables que tuvieron mayor influencia en la producción de hongos. Se encontró en el bosque un total de 24 especies, de las cuales tres fueron exclusivas del bosque de Pinus hartwegii y nueve del bosque de Abies religiosa y 12 especies fueron comunes a ambos tipos de bosques. Asimismo, se observó en el bosque de Pinus un total de 15 especies, cuya producción promedio fue de -107.3 kg·ha-1, con un valor económico de $5,962.00·ha·año-1. En el bosque de Abies se encontraron un total de 21 especies, con una producción total de 214.10 kg·ha-1·año-1, y un valor económico de $9,744. 50·ha·año-1, a precios de 1987.

  10. LAHAN BASAH BUATAN SEBAGAI MEDIA PENGOLAHAN AIR LIMBAH BUDIDAYA UDANG VANAME (Litopenaeus vannamaei BERSALINITAS RENDAH (Constructed Wetland for Remediation of Brackish Wastewater from White Shrimp (Litopenaeus vannamaei Cultivation

    Directory of Open Access Journals (Sweden)

    Syafrudin Raharjo

    2015-07-01

    Full Text Available ABSTRAK Air limbah budidaya udang berjumlah relatif banyak dan mengandung bahan pencemar yang berpotensi mencemari lingkungan. Di sisi lain, air limbah tersebut dapat diolah dan diresirkulasi dalam sistem budidaya udang. Tujuan penelitian ini adalah untuk menyelidiki kemampuan sistem lahan basah buatan-aliran air permukaan (LBB-AAP yang ditanami dengan rumput vetiver (Chrysopogon zizanioides, L dalam menghilangkan pencemar (NO2-, NO3-, NH3, NH4+ dan PO43- dari air limbah budidaya udang vaname (Litopenaeus vannamaei kondisi mesohaline dan mengevaluasi kinerja sistem tersebut. Hasil penelitian menunjukkan bahwa sistem LBB-AAP mampu mengeliminasi parameter NO2-, NO3-, NH3, NH4+ dan PO43- secara signifikan. Rumput vetiver mampu tumbuh pada kondisi mesohaline dan dapat melakukan remediasi air limbah tersebut. Serapan rumput vetiver dalam sistem LBB-AAP untuk NO3-, NH4+ dan PO43-adalah 28, 63 dan 83 %. Desain konstruksi LBB-AAP tipe Hidroponik menunjukkan kinerja terbaik dalam pengendalian air limbah budidaya udang vaname dibandingkan dengan tipe emergent, kombinasi hidroponik dan emergent. ABSTRACT The amount of wastewater shrimp cultivation is relatively/too much, contains a variety of pollutants and potentially pollute the environment. In other side, The wastewater can be treated and also recirculated in shrimp cultivation systems. The purpose of research is to investigate the ability of flow water surface-constructed wetland system (FWS-CWs that planted vetiver grass (Chrysopogon zizanioides, L that removes of pollutants (NO2-, NO3-, NH3, NH4+ and PO43- from wastewater vaname shrimp cultivation (Litopenaeus vannamaei on conditions mesohaline and with the aim of evaluating the performance of the system. The results of the research indicate that FWS-CWs able to eliminate the parameters significantly of NO2-, NO3-, NH3, NH4+ and PO43-. Vetiver grass could grow on mesohaline conditions and it can perform remediation of the wastewater. Uptake of

  11. Leishmaniose tegumentar alviericana na Ilha Grande, Rio de Janeiro: III. reservatórios silvestres e comensais

    Directory of Open Access Journals (Sweden)

    Nelson A. de Araújo Filho

    1981-12-01

    Full Text Available Em 203 animais silvestres e comensais examinados na Praia Vermelha, Ilha Grande, município de Angra dos fíeis, RJ, durante o estudo de um surto de Leishmaniose Tegumentar Americana, foram encontrados 2 exemplares de Proechimys dimidiatus, com lesões hipocrômicas nas extremidades das orelhas, e 1 exemplar de Rattus norvegicus norvegicus, com úlcera de dorso, cuja histopatologia revelou a presença de Leishmania sp. nos 3 exemplares.Of the 203 domestic and wild animais examined in Praia Vermelha, during a study of an outbreak of mucocutaneous leishmaniasis 2 cases of Proechimus dimidiatus were found with hipocromic lesions in the ear extremities and one case of Rattus norvergicus with a back ulcer. Histopathology has shown the presence of Leishmania sp. in the three specimens.

  12. Ecología de la fauna silvestre de la sierra nevada y la Sierra del Ajusco

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    D. Granados Sánchez

    2004-01-01

    Full Text Available Se presenta un estudio sobre la fauna silvestre de la Sierra del Ajusco y la Sierra Nevada, localizadas dentro de la Faja Volcánica Transmexicana (FVT, en la porción oriental del límite meridional de la Cuenca de México. La enorme riqueza biológica de esta zona que rodea la zona metropolitana de la ciudad de México, uno de los mayores complejos urbanos del mundo, ha sobrevivido durante décadas al impacto de la urbanización de las áreas forestales, explotación de recursos naturales, contaminación, cacería, incendios y pastoreo. A pesar de esto, se desconocen muchos de los factores que regulan la dinámica de los ecosistemas en esta región. Con relación a la fauna silvestre la carencia de información es mucho más evidente, aspectos elementales como la diversidad de especies, las interacciones ecológicas, la función de los organismos en el ecosistema, las condiciones de estrés a que están sometidos, han sido poco explorados. En esta investigación se recurrió a observaciones de campo y a la consulta de diferentes fuentes bibliográficas para estimar la riqueza de especies de mamíferos, aves, reptiles y anfibios; las relaciones ecológicas entre los mismos y las consecuencias de la modificación de su hábitat producto de las actividades humanas.

  13. Ácidos grasos y parámetros de calidad del aceite de semilla de uva silvestre (Vitis spp.

    Directory of Open Access Journals (Sweden)

    O. Franco-Mora

    2015-01-01

    Full Text Available México es centro de origen de varias especies de Vitis. La gran mayoría de ellas no son empleadas en la agricultura e industrias derivadas. Como parte de la exploración de su potencial agroindustrial se identificó y cuantificó a los ácidos grasos del aceite de semilla de vid silvestre (Vitis spp. de frutos de Temascaltepec, México y de la accesión E-201, cultivada en Zumpahuacán, México. El contenido promedio de aceite en la semilla fue de 16,7%, encontrando ácido linoleico (71,5%, oleico (17,2%, palmítico (6,6% y esteárico (4,3%. En el aceite obtenido en vides de Temascaltepec se determinó índice de yodo (57,9 g/100 g, índice de saponificación (170,7 mg/g e índice de peroxidos (30 mEq/kg, valores relacionados posiblemente al contenido de ácidos grasos insaturados. Sin embargo, ese mismo grado de insaturación no concordó con el punto de humeo observado (211 °C, lo cual posiblemente está relacionado con el contenido de ácidos palmítico y esteárico. El aceite de semilla de vid silvestre cumple parcialmente con los estándares establecidos para el aceite de semilla de V. vinifera, lo cual implica su potencial culinario, agroindustrial y cosmetológico.

  14. Ixodídeos em animais silvestres na Região do Planalto Serrano, Estado de Santa Catarina, Brasil

    Directory of Open Access Journals (Sweden)

    Marcia Sangaletti Lavina

    2015-10-01

    Full Text Available O conhecimento da fauna de ixodídeos, bem como a potencialidade como transmissores de patógenos aos seus hospedeiros e em alguns casos aos humanos, é de grande importância para o estabelecimento de programas de saúde pública e vigilância epidemiológica. Com o objetivo de identificar ixodídeos de mamíferos silvestres no Planalto Serrano de Santa Catarina foram examinados carrapatos, coletados ou recebidos no Laboratório de Parasitologia e Doenças Parasitárias do Centro de Ciências Agroveterinárias – CAV/UDESC no período 2001 a 2011. Os ixodídeos eram provenientes de animais silvestres que passaram pelo processo de triagem do Hospital de Clínicas Veterinárias (HCV do CAV e de animais encontrados mortos em rodovias do Estado. De acordo com as chaves dicotômicas específicas identificou-se Amblyomma aureolatum coletados em Pseudalopex gymnocercus, Lycalopex gymnocercus, Leopardus pardalis, Cerdocyon thous, Leopardus tigrinus, Puma concolor e Bufo sp.; A. dubitatum em Hydrochoerus hydrochoeris; A. rotundatum em Puma concolor e Bohtrops sp.; A. ovale em Bufo sp.; e Rhipicephalus microplus em Mazama gouazoubira. Foi identificado um exemplar de A. longirostre que se encontrava em uma residência da área urbana do município de Lages. As espécies A. rotundatum em Bohtrops sp. e em Puma concolor; e A. longirostre, encontrada no ambiente, foram relatadas pela primeira vez no estado de Santa Catarina, Brasil. 

  15. Quality of antimicrobial products used in white leg shrimp (Litopenaeus vannamei) aquaculture in Northern Vietnam

    DEFF Research Database (Denmark)

    Tran, Kim Chi; Tran, Minh Phu; Phan, Thi Van

    2018-01-01

    . The documented poor quality of antimicrobial products and inadequate labelling has negative impacts on effective disease treatment; contribute to development of antimicrobial resistance, and the use of such products is associated with food safety and occupational health hazards. There is an urgent need......Antimicrobials are important to treat diseases in aquaculture and the objective of this study was to evaluate the quality of antimicrobial products commonly used in white leg shrimp (Litopenaeus vannamei) aquaculture in Northern Vietnam. A total of 25 antimicrobial products were obtained from 20...... to analyse the concentration of the declared antimicrobials. Results revealed that only 1/12 products with a single antimicrobial contained an active substance within ± 10% (accepted level of variation) of the concentration declared on the product label. More than half of the products contained antimicrobial...

  16. Effect of high hydrostatic pressure on the microbiological, biochemical characteristics of white shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Li, Xiu-Xia; Tian, Xin; Li, Jian-Rong

    2016-06-01

    Using thermal processing (TP) treatment (100 ℃, 1-8 min) as a control, the effects of high hydrostatic pressure (HHP, 200-500 MPa, 2.5-20 min) on the microbiological and biochemical characteristics of white shrimp Litopenaeus vannamei were investigated. The results showed that the efficiency of polyphenol oxidase (PPO) inactivation and log reduction of total plate count (TPC) by HHP treatment were all significantly lower than by TP treatment (p pressure and holding time (p pressure of 300-500 MPa was higher than TP-treated samples, while the yield loss of HHP treatment was significantly lower than with TP treatment (p high pressure of HHP treatment turned the appearance of shrimps slightly pink. © The Author(s) 2015.

  17. Effects of Astaxanthin from Litopenaeus Vannamei on Carrageenan-Induced Edema and Pain Behavior in Mice.

    Science.gov (United States)

    Kuedo, Zulkiflee; Sangsuriyawong, Anantita; Klaypradit, Wanwimol; Tipmanee, Varomyalin; Chonpathompikunlert, Pennapa

    2016-03-19

    Carrageenan produces both inflammation and pain when injected in mouse paws via enhancement of reactive oxygen species formation. We have investigated an effect of astaxanthin extracted from Litopenaeus vannamei in carrageenan-induced mice paw edema and pain. The current study demonstrates interesting effects from astaxanthin treatment in mice: an inhibition of paw edema induced in hind paw, an increase in mechanical paw withdrawal threshold and thermal paw withdrawal latency, and a reduction in the amount of myeloperoxidase enzyme and lipid peroxidation products in the paw. Furthermore the effect was comparable to indomethacin, a standard treatment for inflammation symptoms. Due to adverse effects of indomethacin on cardiovascular and gastrointestinal systems, our study suggests promising prospect of astaxanthin extract as an anti-inflammatory alternative against carrageenan-induced paw edema and pain behavior.

  18. Antimutagenicity and Antiproliferative Studies of Lipidic Extracts from White Shrimp (Litopenaeus vannamei)

    Science.gov (United States)

    Wilson-Sanchez, Griselda; Moreno-Félix, Carolina; Velazquez, Carlos; Plascencia-Jatomea, Maribel; Acosta, Anita; Machi-Lara, Lorena; Aldana-Madrid, María-Lourdes; Ezquerra-Brauer, Josafat-Marina; Robles-Zepeda, Ramón; Burgos-Hernandez, Armando

    2010-01-01

    An organic extract from fresh shrimp (Litopenaeus vannamei) was studied for antimutagenic and antiproliferative properties using Salmonella typhimurium tester strains TA98 and TA100 with metabolic activation (S9) and a cancer cell line (B-cell lymphoma), respectively. Shrimp extract was sequentially fractionated by thin layer chromatography (TLC) and each fraction was tested for antimutagenic and antiproliferative activities. Crude organic extracts obtained from shrimp reduced the number of revertants caused by aflatoxina B1, showing a dose-response type of relationship. Sequential TLC fractionation of the active extracts produced several antimutagenic and/or antiproliferative fractions. These results suggested that the lipid fraction of the tested species contained compounds with chemoprotective properties that reduce the mutagenicity of AFB1 and proliferation of a cancer cell line. PMID:21139845

  19. Genetic improvement of Pacific white shrimp (Penaeus (Litopenaeus vannamei: perspectives for genomic selection

    Directory of Open Access Journals (Sweden)

    Héctor eCastillo-Juárez

    2015-03-01

    Full Text Available The use of breeding programs for the Pacific white shrimp (Penaeus (Litopenaeus vannamei based on mixed linear models with pedigreed data are described. The application of these classic breeding methods yielded continuous progress of great value to increase the profitability of the shrimp industry in several countries. Recent advances in such areas as genomics in shrimp will allow for the development of new breeding programs in the near future that will increase genetic progress. In particular, these novel techniques may help increase disease resistance to specific emerging diseases, which is today a very important component of shrimp breeding programs. Thanks to increased selection accuracy, simulated genetic advance using genomic selection for survival to a disease challenge was up to 2.6 times that of phenotypic sib selection.

  20. The effect of three culture methods on intensive culture system of pacific white shrimp ( Litopenaeus vannamei)

    Science.gov (United States)

    Ma, Zhen; Wan, Rong; Song, Xiefa; Gao, Lei

    2013-09-01

    Different culture methods may affect the intensive culture system of Pacific white shrimp ( Litopenaeus vannamei) regarding water quality and growth and economic performance. This study evaluated the potential effects of three culture methods through cultivation of juvenile shrimps under consistent tank management conditions for 84 d. The three methods involved shrimp cultivation in different tanks, i.e., outdoor tanks with cement bottom (mode-C), greenhouse tanks with cement bottom (mode-G) and outdoor tanks with mud-substrate (mode-M). Results showed that water temperature was significantly higher in mode-G than that in mode-C ( P 0.05), mode-M had significantly higher shrimp yield, survival rate and feed conversion rate ( P vannamei.

  1. Antimutagenicity and Antiproliferative Studies of Lipidic Extracts from White Shrimp (Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Carolina Moreno-Félix

    2010-11-01

    Full Text Available An organic extract from fresh shrimp (Litopenaeus vannamei was studied for antimutagenic and antiproliferative properties using Salmonella typhimurium tester strains TA98 and TA100 with metabolic activation (S9 and a cancer cell line (B-cell lymphoma, respectively. Shrimp extract was sequentially fractionated by thin layer chromatography (TLC and each fraction was tested for antimutagenic and antiproliferative activities. Crude organic extracts obtained from shrimp reduced the number of revertants caused by aflatoxina B1, showing a dose-response type of relationship. Sequential TLC fractionation of the active extracts produced several antimutagenic and/or antiproliferative fractions. These results suggested that the lipid fraction of the tested species contained compounds with chemoprotective properties that reduce the mutagenicity of AFB1 and proliferation of a cancer cell line.

  2. Carbohydrate Moieties and Cytoenzymatic Characterization of Hemocytes in Whiteleg Shrimp Litopenaeus vannamei

    Science.gov (United States)

    Velázquez, Edwin; Rodríguez-Jaramillo, Carmen; Ascencio, Felipe

    2016-01-01

    Hemocytes represent one of the most important defense mechanisms against foreign material in Crustacea and are also involved in a variety of other physiological responses. Fluorescent lectin-binding assays and cytochemical reactions were used to identify specificity and distribution of carbohydrate moieties and presence of several hydrolytic enzymes, in hemocytes of whiteleg shrimp Litopenaeus vannamei. Two general classes of circulating hemocytes (granular and agranular) exist in L. vannamei, which express carbohydrates residues for FITC-conjugated lectins WGA, LEA, and PNA; UEA and Con-A were not observed. Enzymatic studies indicated that acid phosphatase, nonspecific esterase, and specific esterases were present; alkaline phosphatase was not observed. The enzymes and carbohydrates are useful tools in hemocyte classification and cellular defense mechanism studies. PMID:27833641

  3. Molecular cloning and characterization of a threonine/serine protein kinase lvakt from Litopenaeus vannamei

    Science.gov (United States)

    Ruan, Lingwei; Liu, Rongdiao; Xu, Xun; Shi, Hong

    2014-07-01

    The phosphatidylinositol 3-kinase (PI3K)-AKT pathway is involved in various cellular functions, including anti-apoptosis, protein synthesis, glucose metabolism and cell cycling. However, the role of the PI3K-AKT pathway in crustaceans remains unclear. In the present study, we cloned and characterized the AKT gene lvakt from Litopenaeus vannamei. The 511-residue LVAKT was highly conserved; contained a PH domain, a catalytic domain and a hydrophobic domain; and was highly expressed in the heart and gills of L. vannamei. We found, using Real-Time Quantitative PCR (Q-PCR) analysis, that lvakt was up-regulated during early white spot syndrome virus (WSSV) infection. Moreover, the PI3K-specific inhibitor, LY294002, reduced viral gene transcription, implying that the PI3K-AKT pathway might be hijacked by WSSV. Our results therefore suggest that LVAKT may play an important role in the shrimp immune response against WSSV.

  4. Effects of Dietary Lactobacillus plantarum on Growth Performance, Digestive Enzymes and Gut Morphology of Litopenaeus vannamei.

    Science.gov (United States)

    Zheng, Xiaoting; Duan, Yafei; Dong, Hongbiao; Zhang, Jiasong

    2017-07-15

    A 15-day feeding trial was conducted to investigate the effect of dietary Lactobacillus plantarum on growth performance, digestive enzyme activities and gut morphology of juvenile Pacific white shrimp, Litopenaeus vannamei (initial body weight = 7.96 ± 0.59 g). Four microbound diets were formulated to contain fermentation supernatant (FS), live bacteria (LB), dead bacteria (DB), and cell-free extract (CE) of L. plantarum. Results indicated that final weight was significantly higher in FS, DB, and CE group in comparison to the control group (P vannamei via the improvement of digestive enzyme activities and the enterocytes height of shrimp. The results of this study will be essential to promote application of probiotics in shrimp aquaculture.

  5. A comparative study of intensive Litopenaeus vannamei culture on four bottom substrates without water change

    Science.gov (United States)

    Shan, Hongwei; Zhang, Li; Gao, Lei; Su, Yuepeng; Bao, Weiyang; Ma, Shen

    2014-08-01

    The effect of four bottom substrates, oyster shell powder (OP), sugarcane bagasse (SB), a mixture of OP and SB (OS) and fresh soil (FS), on the water quality and bacterial and zooplankton density of intensive shrimp ( Litopenaeus vannamei) culture tanks without water change and the growth performance of cultured shrimp were compared in this study. At the end of a 110 days culturing trial, the total ammonium-N (TAN) of the water on SB and the nitrite nitrogen (NO2-N) on OS was significantly lower than that on the other substrates ( Pvannamei without water change than OP and FS. To our knowledge, this study presents the first evidence regarding the effect of different bottom substrates on intensive shrimp culture.

  6. Essential roles of Cdc42 and MAPK in cadmium-induced apoptosis in Litopenaeus vannamei

    Energy Technology Data Exchange (ETDEWEB)

    Peng, Ting; Wang, Wei-Na, E-mail: weina63@aliyun.com; Gu, Mei-Mei; Xie, Chen-Ying; Xiao, Yu-Chao; Liu, Yuan; Wang, Lei

    2015-06-15

    Highlights: • Cd{sup 2+} induces Cdc42 and MAPKs pathway related gene of Litopenaeus vannamei up-regulation. • Reduction of THC, increase of ROS production and apoptotic cell rate were observed when the shrimps exposure to Cd{sup 2+}. • DsRNA-suppression of LvCdc42 and MAPKs during Cd{sup 2+} stress reduces the ROS production and apoptosis. • We conclude that LvCdc42 and MAPKs play key roles in Cd{sup 2+} stress responses of shrimps. - Abstract: Cadmium, one of the most toxic heavy metals in aquatic environments, has severe effects on marine invertebrates and fishes. The MAPK signaling pathway plays a vital role in stress responses of animals. The mitogen-activated protein kinase (MAPK) signaling pathway plays a vital role in animals’ stress responses, including mediation of apoptosis induced by the Rho GTPase Cdc42. However, there is limited knowledge about its function in shrimps, although disorders exacerbated by environmental stresses (including heavy metal pollution) have caused serious mortality in commercially cultured shrimps. Thus, we probed roles of Cdc42 in Litopenaeus vannamei shrimps (LvCdc42) during cadmium exposure by inhibiting its expression using dsRNA-mediated RNA interference. The treatment successfully reduced expression levels of MAPKs (including p38, JNK, and ERK). Cadmium exposure induced significant increases in expression levels of LvCdc42 and MAPKs, accompanied by reductions in total hemocyte counts (THC) and increases in apoptotic hemocyte ratios and ROS production. However, all of these responses were much weaker in LvCdc42-suppressed shrimps, in which mortality rates were higher than in controls. Our results suggest that the MAPK pathway plays a vital role in shrimps’ responses to Cd{sup 2+}. They also indicate that LvCdc42 in shrimps participates in its regulation, and thus plays key roles in ROS production, regulation of apoptosis and associated stress responses.

  7. Purification and characterization of a lectin from the white shrimp Litopenaeus setiferus (Crustacea decapoda) hemolymph.

    Science.gov (United States)

    Alpuche, Juan; Pereyra, Ali; Agundis, Concepción; Rosas, Carlos; Pascual, Cristina; Slomianny, Marie-Christine; Vázquez, Lorena; Zenteno, Edgar

    2005-06-20

    A 291-kDa lectin (LsL) was purified from the hemolymph of the white shrimp Litopenaeus setiferus by affinity chromatography on glutaraldehyde-fixed stroma from rabbit erythrocytes. LsL is a heterotetramer of two 80-kDa and two 52-kDa subunits, with no covalently-liked carbohydrate, and mainly composed by aspartic and glutamic acids, glycine and alanine, with relatively lower methionine and cysteine contents. Edman degradation indicated that the NH2-terminal of the 80-kDa subunit is composed DASNAQKQHDVNFLL, whereas the NH2-terminal of the 52-kDa subunit is blocked. The peptide mass fingerprint of LsL was predicted from tryptic peptides from each subunit by MALDI-TOF, and revealed that each subunit showed 23 and 22%, respectively, homology with the hemocyanin precursor from Litopenaeus vannamei. Circular dichroism analysis revealed beta sheet and alpha helix contents of 52.7 and 6.1%, respectively. LsL agglutinate at higher titers guinea pig, murine, and rabbit erythrocytes its activity is divalent cation-dependent. N-acetylated sugars, such as GlcNAc, GalNAc, and NeuAc, were the most effective inhibitors of the LsL hemagglutinating activity. Sialylated O-glycosylated proteins, such as bovine submaxillary gland mucin, human IgA, and fetuin, showed stronger inhibitory activity than sialylated N-glycosylated proteins, such as human orosomucoid, IgG, transferrin, and lactoferrin. Desialylation of erythrocytes or inhibitory glycoproteins abolished their capacity to bind LsL, confirming the relevance of sialic acid in LsL-ligand interactions.

  8. Osmoregulatory capacity of the shrimp Litopenaeus vannamei at different temperatures and salinities, and optimal culture environment

    Directory of Open Access Journals (Sweden)

    L. Fernando Bückle

    2006-09-01

    Full Text Available Osmoregulation in Litopenaeus vannamei was studied in a factorial experiment at four temperatures (20, 24, 28 and 32 °C and six salinities (10, 16, 22, 28, 34 and 40 ‰. The isosmotic related points for 20, 24, 28, and 32 °C were 754, 711, 822, and 763 mmol/kg, respectively. This species hyperregulates between at salinities of 10 and 20 ‰ and hyporegulates between 20 and 40 ‰. The isosmotic point in L. vannamei exposed to constant salinities changed in relation to temperature from 717 to 823 mmol/kg. For these experimental conditions, the T-S combination of 32 °C and 28 ‰ produced the best growth. Rev. Biol. Trop. 54 (3: 745-753. Epub 2006 Sept. 29.La respuesta osmorreguladora de Litopenaeus vannamei se estudió en un experimento factorial con cuatro temperaturas (20, 24, 28 y 32 ºC y seis salinidades (10, 16, 22, 28, 34 y 40 ‰. Los puntos isosmóticos relacionados para 20, 24, 28, y 32 ºC fueron 754, 711, 822, y 763 mmol/kg, respectivamente. Esta especie hiperregula dentro del intervalo de 10 y 20 ‰ e hiporegula entre 20 y 40 ‰. El punto isosmótico de L. vannamei expuesto a salinidades constantes cambia en relación a la temperatura desde 717 a 823 mmol/kg. Para estas condiciones experimentales, la combinación T-S de 32 ºC y 28 ‰ produjo el mejor crecimiento.

  9. Recovery of strains of Vibrio parahaemolyticus inoculated in marine shrimp Litopenaeus vannamei exposed to the cooling and freezing temperatures

    OpenAIRE

    Dannielle Batista Rolim Sousa

    2007-01-01

    The aim of this study was to evaluate the survival of Vibrio parahaemolitycus inoculated in meat homogenate of shrimp Litopenaeus vannamei at different temperatures of refrigeration (refrigerator, freezer and isothermic box with ice) during ten days and on the 15th, 20th and 25th days. The experiment was repeated six times during October 2005 to March 2006. Shrimps were obtained on fish market located at Praia do Mucuripe, Fortaleza, CearÃ. In the laboratory, the shrimps wer...

  10. Experimental evaluation of co-culture of juvenile sea cucumbers, Holothuria scabra (Jaeger), with juvenile blue shrimp, Litopenaeus stylirostris (Stimpson)

    OpenAIRE

    Purcell, S; Patrois, Jacques; Fraisse, N.

    2006-01-01

    The co-culture of juvenile sea cucumber Holothuria scabra (Jaeger), or 'sandfish', with juvenile blue shrimp Litopenaeus stylirostris (Stimpson) was tested by growing groups in co-culture and monoculture for 3 weeks in tanks with enriched sand substratum. Feed was supplied on trays, accessible only to shrimp. Survival of shrimp and sandfish was high in all treatments (73-100%). Growth of shrimp did not differ between monoculture and co-culture, but sandfish grew significantly slower in co-cul...

  11. Transcriptome analysis on the exoskeleton formation in early developmetal stages and reconstruction scenario in growth-moulting in Litopenaeus vannamei

    OpenAIRE

    Yi Gao; Jiankai Wei; Jianbo Yuan; Xiaojun Zhang; Fuhua Li; Jianhai Xiang

    2017-01-01

    Exoskeleton construction is an important issue in shrimp. To better understand the molecular mechanism of exoskeleton formation, development and reconstruction, the transcriptome of the entire developmental process in Litopenaeus vannamei, including nine early developmental stages and eight adult-moulting stages, was sequenced and analysed using Illumina RNA-seq technology. A total of 117,539 unigenes were obtained, with 41.2% unigenes predicting the full-length coding sequence. Gene Ontology...

  12. The microsporidian Enterocytozoon hepatopenaei is not the cause of white feces syndrome in whiteleg shrimp Penaeus (Litopenaeus) vannamei

    OpenAIRE

    Tangprasittipap, Amornrat; Srisala, Jiraporn; Chouwdee, Saisunee; Somboon, Montagan; Chuchird, Niti; Limsuwan, Chalor; Srisuvan, Thinnarat; Timothy W Flegel; Sritunyalucksana, Kallaya

    2013-01-01

    Background The microsporidian Enterocytozoon hepatopenaei was first described from Thailand in 2009 in farmed, indigenous giant tiger shrimp Penaeus (Penaeus) monodon. The natural reservoir for the parasite is still unknown. More recently, a microsporidian closely resembling it in morphology and tissue preference was found in Thai-farmed, exotic, whiteleg shrimp Penaeus (Litopenaeus) vannamei exhibiting white feces syndrome (WFS). Our objective was to compare the newly found pathogen with E. ...

  13. Modified extensive pond culture of Litopenaeus vannamei for sustainable shrimp culture in the Philippines

    OpenAIRE

    Cecilia J. Jaspe; Christopher M. A. Caipang; Bessie J. G. Elle

    2011-01-01

    The shrimp culture industry provides huge revenues to most aquaculture producing countries,but it is also beset with problems that hamper its sustainability. In the present study, we described amodified extensive pond culture method for white shrimp, Litopenaeus vannamei in the Philippines duringthe wet and dry months. One hectare earthen ponds were prepared and added with organic and/orinorganic fertilizers to stimulate natural food production. The ponds were stocked with L. vannameipostlarv...

  14. Growth and immune response of Litopenaeus vannamei fed on β-(1, 3 glucan and poly-β hydroxybutyrate

    Directory of Open Access Journals (Sweden)

    , Sarmin

    2015-05-01

    Full Text Available ABSTRACT This research was aimed to examine the growth performance and non-specific immune response of Pacific white shrimp (Litopenaeus vannamei fed on the diet supplemented with β-(1,3 glucan (BG and poly-β-hydroxybutyrate (PHB as feed additives. Shrimp juvenile at an initial body weight of 2.06±0.03 g was randomly distributed into 12 units of aquaria at a density of 20 shrimps/tank and reared for 42 days. The treatments applied in this study were control (without feed additives, 1.5 g/kg BG, 10 g/kg PHB and 1,5 g/kg BG+10 g/kg PHB. Results showed that shrimp fed on 1.5 /kg BG-supplemented feed had significantly higher growth performance and non-specific immune response. Keywords: growth, shrimp, non-specific immune response, Litopenaeus vannamei  ABSTRAK Penelitian ini bertujuan untuk menguji kinerja pertumbuhan dan respons imun nonspesifik udang vaname Litopenaeus vannamei yang diberi pakan dengan penambahan feed additive berupa β-(1,3 glukan (BG dan poli-β-hidroksibutirat (PHB. Juvenil udang 2,06±0,03 g dipelihara pada 12 unit akuarium dengan empat perlakuan dan tiga ulangan, serta padat tebar 20 ekor/tank selama 42 hari pemeliharaan. Perlakuan yang diberikan dalam penelitian ini yaitu penambahan BG (1,5 g/kg, PHB (10 g/kg, dan BG (1,5 g/kg+PHB (10 g/kg, serta kontrol (tanpa penambahan feed additive. Hasil penelitian menunjukkan bahwa udang yang diberi 1,5 g/kg BG memiliki kinerja pertumbuhan dan respons imun nonspesifik yang terbaik. Kata kunci: pertumbuhan, udang, respons imun nonspesifik, Litopenaeus vannamei

  15. Aproximación al uso y tráfico de fauna silvestre en Puerto Carreño, Vichada, Colombia.

    OpenAIRE

    Cruz Antía, Daniel; Gomez, Juan Ricardo

    2011-01-01

    El tráfico ilegal de vida silvestre es un problema que enfrentan los países de alta diversidad biológica, como es el caso de Colombia. La extracción directa de individuos causa disminuciones en las poblaciones naturales y, en algunos casos, llevan a las especies a estados críticos e incluso a la extinción. Una de las regiones, en Colombia, donde es común encontrar una relación directa de extracción-uso y tráfico de vida silvestre, es en la región de la Orinoquia. Este estudio presenta los res...

  16. Estudio preliminar de especies de micobacterias en primates colombianos no humanos en cautiverio en dos centros de rescate de fauna silvestre de Bogotá

    Directory of Open Access Journals (Sweden)

    Barragán Fonseca Karol Bibiana

    2005-07-01

    Full Text Available Se determinó la presencia de diferentes especies de micobacterias en 83 primates colombianos en cautiverio en dos centros de rescate de fauna silvestre de Bogotá. Se evaluaron 166 muestras de sangre y lavado gástrico mediante amplificación por PCR-spoligotyping para micobacterias del
    complejo tuberculosis y Hsp65-PRA para micobacterias no  tuberculosas (MNT. Se obtuvo una prevalencia del 2,4% para micobacterias del complejo Mycobacterium tuberculosis, encontrando un Saimiri sciureus infectado con Mycobacterium microti tipo vole y un Saguinus leucopus infectado con
    Mycobacterium africanum, siendo el primer reporte de estas micobacterias para primates del Nuevo Mundo. La prevalencia para MNT fue de 7,2% perteneciendo a las especies Mycobacterium phlei, M. terrae, M. vaccae, M. flavescens, M. flavescens y M. zsulgaii, algunas de estas, patógenos oportunistas. No existe una diferencia a nivel descriptivo en cuanto a la edad, al sexo y al tipo de entrega de los individuos infectados con MNT y micobacterias tuberculosas con respecto a los individuos que no las presentaron. Los primates del Nuevo Mundo pueden ser portadores sanos de micobacterias tuberculosas lo que representa un riesgo para la salud humana, y con ciertas especies de micobacterias, podría ser un riesgo para las poblaciones de primates silvestres en caso de que estos primates sean liberados en el medio silvestre. Este estudio es relevante para el país, ya que integra conceptos médicos tanto epidemiológicos como microbiológicos, con aspectos relacionados con la conservación de la fauna silvestre.

  17. Actividad citotóxica, anti-inflamatoria y anti-ulcerogénica de plantas silvestres e in vitro de castilleja tenuiflora benth.

    OpenAIRE

    Sanchez Ocampo, Paul Mauricio

    2016-01-01

    Castilleja tenuiflora Benth. (Orobanchaceae) es una planta usada en la medicina tradicional mexicana para tratar tumores, esterilidad, desordenes gastrointestinales y cirrosis. Se han identificado iridoides, feniletanoides y flavonoides en plantas silvestres y en cultivos in vitro de C. tenuiflora, los cuales están asociados a diversas actividades biológicas como antioxidante, antitumoral, citotóxica, anti-inflamatoria e inmunoestimulante. Por estos antecedentes la planta se pr...

  18. Estudio preliminar de especies de micobacterias en primates colombianos no humanos en cautiverio en dos centros de rescate de fauna silvestre de Bogotá

    Directory of Open Access Journals (Sweden)

    Karol Bibiana Barragán Fonseca

    2005-01-01

    Mycobacterium africanum, siendo el primer reporte de estas micobacterias para primates del Nuevo Mundo. La prevalencia para MNT fue de 7,2% perteneciendo a las especies Mycobacterium phlei, M. terrae, M. vaccae, M. flavescens, M. flavescens y M. zsulgaii, algunas de estas, patógenos oportunistas. No existe una diferencia a nivel descriptivo en cuanto a la edad, al sexo y al tipo de entrega de los individuos infectados con MNT y micobacterias tuberculosas con respecto a los individuos que no las presentaron. Los primates del Nuevo Mundo pueden ser portadores sanos de micobacterias tuberculosas lo que representa un riesgo para la salud humana, y con ciertas especies de micobacterias, podría ser un riesgo para las poblaciones de primates silvestres en caso de que estos primates sean liberados en el medio silvestre. Este estudio es relevante para el país, ya que integra conceptos médicos tanto epidemiológicos como microbiológicos, con aspectos relacionados con la conservación de la fauna silvestre.

  19. Optimización del proceso de obtención de biodiesel a partir de colza silvestre (Brassica Campestris

    Directory of Open Access Journals (Sweden)

    Héctor Ramírez

    2012-01-01

    Full Text Available Los objetivos de esta investigación fueron , optimizar el rendimiento de biodiesel a partir del aceite de colza silvestre en función de la relación molar metanol/aceite, la concentración de catalizadores homogéneos NaOH y KOH, te mperatura y tiempo de transesterificación a través de la metodología de superficie respuesta, y determinar las características fisicoquímicas del biodiesel obtenido en condiciones optimizadas. Fue aplic ado un diseño de Plackett y Burman (PB12 para la etap a d e screening y un diseño compuesto central rotacional (DCCR para la optimización final. Las condiciones que maximizan el r endimiento de biodiesel (77.8 % se obtuvieron a concentraciones de 0 – 0.2 % de NaOH y de 0.4 a 0.6 % de KOH , con tiempos de 77 a 81 minutos, manteniendo constante la relación molar metanol/aceite en 6/1 y una temperatura de 60ºC. Las propiedades fisicoquímicas del biodiesel obtenido en condiciones optimizadas cumplen con las especificaciones técnicas dadas por la ASTM D6751 – 07 y EN14214.

  20. Tráfico y tenencia ilegal de fauna silvestre en el departamento de Boyacá

    Directory of Open Access Journals (Sweden)

    Alejandro Cabrejo Bello

    2010-10-01

    Full Text Available Con el fin de establecer cuáles son los principales grupos taxonómicos, suparticipación y las ciudades con mayores índices de tráfico y tenencia ilegal de vida silvestre en el departamento de Boyacá, jurisdicción de la Corporación Autónoma Regional de Boyacá- Corpoboyacá, se tomaron reportes de la base de datos de dicha entidad entre los años 2004 y 2008. Se hallaron registros de 398 individuos decomisados por parte de la autoridad ambiental y otros por entregas voluntarias. El grupo con mayor incidencia fue el de las aves con 58%,seguido por los reptiles con 26% y en tercer lugar los mamíferos con el 16% de los registros. Se observó que las tres principales ciudades del departamento (Tunja, Duitama y Sogamoso reportan las frecuencias más elevadas de incautaciones. Puerto Boyacá, también registra un número elevado de decomisos. Dentro de los tres grupos mencionados, las especies más afectadas correspondena los órdenes Psitaciformes, Primates y Testudínata, por lo cual merecen principal atención. Se recomienda además, implementar planes de conservación y emprender actividades de educación ambiental encaminadas a la utilización sostenible de la fauna en general.

  1. Salmonella spp. em Cebus spp. mantidos em Centros de Triagem de Animais Silvestres no Nordeste do Brasil

    Directory of Open Access Journals (Sweden)

    Débora Rochelly Alves Ferreira

    2011-12-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2012v25n2p181 Objetivou-se com esse estudo isolar e identificar os sorovares de Salmonella spp. em Cebus spp. mantidos em cativeiro. Foram investigados 28 animais mantidos em Centros de Triagem de Animais Silvestres (CETAS do Nordeste do Brasil. Foram realizados suabes retais que foram processados para o isolamento da bactéria em meios seletivos seguido da identificação dos sorovares por meio de provas bioquímicas. Das 28 amostras analisadas, cinco (17,9% foram positivas para Salmonella spp. e os sorovares identificados foram Infantis (40%, Panama (40% e Newport (20%. Ocorreu associação significativa (p<0.05 para as variáveis: instituição (p=0,026, armazenamento de frutas, verduras e hortaliças (p=0,026, fornecimento de carne crua (p=0,026, tipo de bebedouro (p=0,026 e higienização diária de bebedouros (p=0,026. Esse estudo demonstrou que infecções por Salmonella spp. ocorrem em populações de Cebus mantidos em cativeiro e apontou para a necessidade de se realizar investigações periódicas em todo o plantel, assim como implantar medidas de controle para evitar riscos à saúde dos animas e pública.

  2. Fenología de la floración en tomate cultivado y especies silvestres relacionadas

    Directory of Open Access Journals (Sweden)

    Edwin Fernando Restrepo Salazar

    2008-06-01

    Full Text Available En un diseño experimental de bloques completos al azar se comparó la fenología de la floración de doce accesiones silvestres de Solanum sección Lycopersicum con la del tomate cultivado tipo “chonto”. Los tratamientos se repitieron tres veces. Se evaluó la aparición de las 12 primeras inflorescencias y el tipo de inflorescencia. Las fenologías de las accesiones de la variedad glabratum de S. habrochaites (PI 134417, PI134418 y PI126449, las accesiones LA1624, LA2092 de la variedad typicum de S. habrochaites y la accesión LA 444-1 de S. peruvianum coincidieron con la del tomate “chonto”. No se presentaron diferencias significativas en la variable días a inicio de floración entre las accesiones de la variedad glabratum y el tomate cultivado. Las accesiones de la variedad glabratum de S. habrochaites y el tomate cultivado presentaron inflorescencias simples; mientras que las accesiones de la variedad typicum de S. habrochaites y de la especie S. peruvianum presentaron inflorescencias bifurcadas.

  3. Aquacultural importance of the integumental pore pattern in postlarval whiteleg shrimp Litopenaeus vannamei (Decapoda: Penaeidae

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    Jorge Alberto Cabrera-Jiménez

    2006-09-01

    Full Text Available The analysis of integumental pore pattern has identified two intraspecific groups of postlarval shrimp Litopenaeus vannamei (Boone, 1931, a species of which three to five natural populations or groups are expected to be found from Mexico to Peru. Natural distribution and man-made redistributions have not been typified on a population basis in any penaeid, but it is important to do so in this commercially significant group. Important factors to be defined on a geographic population or group basis include differences in domesticity (eg resistance to pathological agents; food conversion indexes and food preferences; growth indices; tolerance to stress of various origins and other attributes useful in aquaculture. This consideration is valid not only for L. vannamei but also for any other shrimp species that has been the object of artificial redistribution. Rev. Biol. Trop. 54 (3: 707-709. Epub 2006 Sept. 29.Mediante el análisis del patrón de poros tegumentarios ha sido posible caracterizar a dos grupos intraespecíficos en dos estadios postlarvales del camarón Litopenaeus vannamei. Se estima que se podrán encontrar entre tres y cinco grupos o poblaciones naturales en el área de distribución natural desde México hasta Perú. En ningún peneido se ha tipificado a los grupos o poblaciones naturales, lo cual es muy importante no solamente en relación con las poblaciones en su medio natural, sino también en relación a las redistribuciones hechas por el ser humano. Esto se debe a las implicaciones acuiculturales y a las diferencias en domesticidad, como la resistencia o sensibilidad a enfermedades virales y a otros agentes patógenos, el factor de conversión y las preferencias alimenticias, el índice de crecimiento, la tolerancia al estrés de diversos orígenes y otros atributos útiles en la acuicultura, todos los cuales no están definidos aún sobre la base de grupos o poblaciones geográficas. Esta consideración es válida no solamente

  4. Direct Nitrous Oxide Emission from the Aquacultured Pacific White Shrimp (Litopenaeus vannamei).

    Science.gov (United States)

    Heisterkamp, Ines M; Schramm, Andreas; de Beer, Dirk; Stief, Peter

    2016-07-01

    The Pacific white shrimp (Litopenaeus vannamei) is widely used in aquaculture, where it is reared at high stocking densities, temperatures, and nutrient concentrations. Here we report that adult L. vannamei shrimp emit the greenhouse gas nitrous oxide (N2O) at an average rate of 4.3 nmol N2O/individual × h, which is 1 to 2 orders of magnitude higher than previously measured N2O emission rates for free-living aquatic invertebrates. Dissection, incubation, and inhibitor experiments with specimens from a shrimp farm in Germany indicated that N2O is mainly produced in the animal's gut by microbial denitrification. Microsensor measurements demonstrated that the gut interior is anoxic and nearly neutral and thus is favorable for denitrification by ingested bacteria. Dinitrogen (N2) and N2O accounted for 64% and 36%, respectively, of the nitrogen gas flux from the gut, suggesting that the gut passage is too fast for complete denitrification to be fully established. Indeed, shifting the rearing water bacterial community, a diet component of shrimp, from oxic to anoxic conditions induced N2O accumulation that outlasted the gut passage time. Shrimp-associated N2O production was estimated to account for 6.5% of total N2O production in the shrimp farm studied here and to contribute to the very high N2O supersaturation measured in the rearing tanks (2,099%). Microbial N2O production directly associated with aquacultured animals should be implemented into life cycle assessments of seafood production. The most widely used shrimp species in global aquaculture, Litopenaeus vannamei, is shown to emit the potent greenhouse gas nitrous oxide (N2O) at a particularly high rate. Detailed experiments reveal that N2O is produced in the oxygen-depleted gut of the animal by bacteria that are part of the shrimp diet. Upon ingestion, these bacteria experience a shift from oxic to anoxic conditions and therefore switch their metabolism to the anaerobic denitrification process, which produces N

  5. Litopenaeus vannamei

    African Journals Online (AJOL)

    STORAGESEVER

    2009-07-20

    Jul 20, 2009 ... Selangor, Malaysia. Accepted 17 March, 2009. The effect of Bacillus subtilis, isolated from digestive tract of Macrobrachium rosenbergii was investigated on .... flasks containing 1000 ml of Tryptone Soy Broth, TSB (Difco, USA) at the same .... yield of product of L. vannamei (Figure 1a), while no significance ...

  6. Antimutagenic Compounds of White Shrimp (Litopenaeus vannamei: Isolation and Structural Elucidation

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    Carmen-María López-Saiz

    2016-01-01

    Full Text Available According to the World Health Organization, cancer is the main cause of mortality worldwide; thus, the search of chemopreventive compounds to prevent the disease has become a priority. White shrimp (Litopenaeus vannamei has been reported as a source of compounds with chemopreventive activities. In this study, shrimp lipids were extracted and then fractionated in order to isolate those compounds responsible for the antimutagenic activity. The antimutagenic activity was assessed by the inhibition of the mutagenic effect of aflatoxin B1 on TA98 and TA100 Salmonella tester strains using the Ames test. Methanolic fraction was responsible for the highest antimutagenic activity (95.6 and 95.9% for TA98 and TA100, resp. and was further separated into fifteen different subfractions (M1–M15. Fraction M8 exerted the highest inhibition of AFB1 mutation (96.5 and 101.6% for TA98 and TA100, resp. and, after further fractionation, four subfractions M8a, M8b, M8c, and M8d were obtained. Data from 1H and 13C NMR, and mass spectrometry analysis of fraction M8a (the one with the highest antimutagenic activity, suggest that the compound responsible for its antimutagenicity is an apocarotenoid.

  7. Nutritive value of diets containing fish silage for juvenile Litopenaeus vannamei (Bonne, 1931).

    Science.gov (United States)

    Gallardo, Pedro; Gaxiola, Gabriela; Soberano, Sofía; Taboada, J Gabriel; Pérez, Marquelia; Rosas, Carlos; Cuzon, Gerard; Espinosa, L Gabriela; Sotelo, Angela

    2012-08-30

    Fish wastes has been used for many years as an alternative in feeds for aquaculture. In the present study weight gain of juvenile white shrimp Litopenaeus vannamei fed diets including fish waste silage (WS), fish waste silage with soybean meal SBM (WS + S) or fish waste meal (WM) was compared. A conventional acidic silage process was applied to obtain from wastes (skin, heads, bones and viscera) of snapper (Lutjanus spp.), grunt (Haemulon plumieri), and grouper (Epinephelus spp.) an ingredient rich in protein. After 3 days ensilage more than 90% protein was hydrolysed. Waste material processed at pH 3.8 lost about 24% tryptophan. Butylated hydroxytoluene (BHT) prevented lipid oxidation, as shown after 45 days with malonaldehyde production. Shrimp fed WS + S diet gained 0.7 g per week higher than those fed WS and WM diets with 0.3 g per week (P < 0.05). WS processed with formic acid under conditions of low pH is beneficial for the white shrimp L. vannamei. It sustained reasonable weight gain combined with soybean meal in practical diets. On the other hand, BHT addition was beneficial in preventing oxidative action during silage preparation. Copyright © 2012 Society of Chemical Industry.

  8. Assessment of viability of sperm cells of Litopenaeus vannamei on cryopreservation

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    Marcela Fornari Uberti

    2014-06-01

    Full Text Available Aiming at assessing the cryopreservation potential of Litopenaeus vannamei sperm cells, 74 spermatophores were manually extracted from the sexually mature individuals. After the toxicity test to define the cryoprotectant concentration, suspensions of spermatic cells were cryopreserved in the groups in freezing solutions comprising different cryoprotectants such as dimethyl sulfoxide (DMSO and ethylene glycol (EG at 10% concentration. Each treatment was divided in subgroups for storage in liquid nitrogen during 0, 30, 60 and 90 days, in triplicate. After thawing at 25ºC for 40 seconds, cell viability in the suspensions was analyzed under the microscope in eosin-nigrosin stain and flow cytometry. There were no significant differences between the cryoprotectants used. For all the treatments, lower and higher mortalities occurred in the 0 and 90 days, respectively. By applying the eosin-nigrosin technique, lower and higher mortalities were 23.17 and 82.11% for DMSO and 29.94 and 83.72% for EG, while the flow cytometry registered mortalities of 2.42 and 55.13% for DMSO and 0.90 and 55.56% for EG. The Spearman correlation coefficient indicated a positive correlation (R=0.91 between the two techniques used. It was concluded that there was a decrease in cell viability within a longer cryopreservation time.

  9. Melanosis and quality changes of Pacific white shrimp (Litopenaeus vannamei) treated with catechin during iced storage.

    Science.gov (United States)

    Nirmal, Nilesh Prakash; Benjakul, Soottawat

    2009-05-13

    Melanosis, microbiological, chemical, and physical changes of Pacific white shrimp (Litopenaeus vannamei) treated with catechin were monitored during iced storage of 10 days. Whole shrimp treated with catechin solution (0.05 or 0.1%) had retarded growth of psychrophilic bacteria and spoilage microorganisms including H(2)S-producing bacteria and enterobacteriaceae throughout storage in comparison with the control and those treated with 1.25% sodium metabisilfite (SMS) (P < 0.05). The lower increases in pH and total volatile base (TVB) content were obtained in the shrimp treated with catechin solution at both levels, compared with those of other samples (P < 0.05). Lipid oxidation, loss in freshness and melanosis were lowered by catechin treatment. In general, the efficacy of catechin in lowering melanosis and quality losses increased with increasing levels used. Additionally, catechin (0.01, 0.05, and 0.1% (w/v)) showed inhibitory activity toward polyphenoloxidase (PPO) of Pacific white shrimp in a dose-dependent manner. Therefore, catechin can be used as a promising melanosis inhibitor as well as an antimicrobial and an antioxidant in ice-stored shrimp.

  10. Pleonal muscle development in the shrimp Penaeus (Litopenaeus) vannamei (Crustacea: Malacostraca: Decapoda: Dendrobranchiata).

    Science.gov (United States)

    Hertzler, Philip L; Freas, William R

    2009-05-01

    Penaeoidean shrimp pleonal muscle is a valuable economic resource worldwide, but little is known of its development during larval stages. The development of pleonal muscle in Penaeus (Litopenaeus) vannamei was studied by rhodamine-phalloidin staining and laser-scanning confocal microscopy. Dorsal pleonal muscle was first evident at the protozoea I stage while ventral pleonal muscle was present by the protozoea II stage. Identifiable ventral pleonal muscles were evident by the protozoea III stage and all ventral muscle types were present in the mysis I. The tail flex response began at the mysis stage and growth of existing pleonal muscles continued. The pleopods formed during the mysis stages, with coxal and basis muscles developed by mysis III. The pleopods became functional beginning with the first post-larval stage. We conclude that the pleonal muscle pattern of P. vannamei larvae is similar to that of adult Penaeus setiferus, and that homologous muscles are present. The major formation of dorsal pleonal muscles occurs during the protozoea II stage, while significant development of ventral pleonal muscles occurs during the protozoea III stage.

  11. Nitrite toxicity of Litopenaeus vannamei in water containing low concentrations of sea salt or mixed salts

    Science.gov (United States)

    Sowers, A.; Young, S.P.; Isely, J.J.; Browdy, C.L.; Tomasso, J.R.

    2004-01-01

    The uptake, depuration and toxicity of environmental nitrite was characterized in Litopenaeus vannamei exposed in water containing low concentrations of artificial sea salt or mixed salts. In 2 g/L artificial sea salts, nitrite was concentrated in the hemolymph in a dose-dependent and rapid manner (steady-state in about 2 d). When exposed to nitrite in 2 g/L artificial sea salts for 4 d and then moved to a similar environment without added nitrite, complete depuration occurred within a day. Increasing salinity up to 10 g/L decreased uptake of environmental nitrite. Nitrite uptake in environments containing 2 g/L mixed salts (combination of sodium, potassium, calcium and magnesium chlorides) was similar to or lower than rates in 2 g/L artificial sea salt. Toxicity was inversely related to total dissolved salt and chloride concentrations and was highest in 2 g/L artificial sea salt (96-h medial lethal concentration = 8.4 mg/L nitrite-N). Animals that molted during the experiments did not appear to be more susceptible to nitrite than animals that did not molt. The shallow slope of the curve describing the relationship between toxicity and salinity suggests that management of nitrite toxicity in low-salinity shrimp ponds by addition of more salts may not be practical. ?? Copyright by the World Aquaculture Society 2004.

  12. Encapsulated Synbiotic Dietary Supplementation at Different Dosages to Prevent Vibriosis in White Shrimp, Litopenaeus vannamei

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    Anis Zubaidah

    2015-10-01

    Full Text Available The aim of this study was to evaluate the effect of encapsulated synbiotic (Bacillus sp. NP5 and oligosaccharide dietary at different dosages on growth performance, survival rate, feed conversion ratio, and immune responses of Litopenaeus vannamei against Vibrio infection. The shrimps of the main treatments were fed by the diet that contained three different dosages of encapsulated synbiotic [0.5% (A, 1% (B, and 2% (C (w/w] with feeding rate of 5% of shrimp biomass (4 times a day. The shrimps of two control treatments (negative control and positive control were fed only by commercial feed without supplementation of encapsulated synbiotic. The growth, feed conversion ratio, and survival rate were observed after 30 days of encapsulated synbiotic dietary. The shrimps were then challenged by injection of Vibrio harveyi (6 log colony forming units/mL 0.1 mL/shrimp, excluded the negative control treatment. Afterward, the survival and immune responses were observed for 9 days after experimental infection. The shrimps treated with 2% encapsulated synbiotic (treatment C in the diet showed the highest growth performance (2.98 ± 0.42%, feed conversion ratio (1.26 ± 0.19, and better immune responses i.e. total hemocyte counts, differential hemocyte count, phenoloxidase, and intestine bacteria observation compared to those of positive control treatment.

  13. Black gill disease of Pacific white leg shrimp (Litopenaeus vannamei by Aspergillus flavus

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    Naresh Kumar Dewangan

    2015-10-01

    Full Text Available Objective: To study the epidemiology of black gill disease in white leg shrimp which is a major problem being faced by the commercial shrimp farmers who are culturing Litopenaeus vannamei (L. vannamei in India. Methods: The normal and infected shrimps were collected from shrimp pond and the gill was preserved in appropriate preservative for histopathological examination and scanning electron microscope analysis. Pathogenic fungus was isolated from black gill of L. vannamei in potato dextrose agar medium. Morphological study and fungal strain identification were done by using light microscopy and scanning electron microscope. Fungal DNA was amplified by ITS4 and ITS5 primers and gene sequencing was done by Macrogen Inc., Korea. Phylogenetic tree was prepared by using MEGA 6 software. Results: Fungal spores and hyphae were observed both in internal and external gill surface of infected shrimps. Fungal spores were round in shape and mature sporangium was observed. The histopathology study showed clearly that infected gill was damaged by the fungi. Scanning electron microscopic study showed adherence of fungi in infected gill. Internal transcribed spacer gene sequencing revealed that it was caused by Aspergillus flavus. Conclusions: The outcome of the present study would help to know the cause of black gill disease and to understand the effect of pathogenic fungi in shrimp culture. This study will initiate researchers for work in field of treatment or prevention of black gill disease in commercial L. vannamei culture.

  14. Increased nucleoside diphosphate kinase activity induces white spot syndrome virus infection in Litopenaeus vannamei.

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    Peng-Fei Liu

    Full Text Available Nucleoside diphosphate kinase (NDK, which has the same sequence as oncoprotein (OP in humans, can induce nucleoside triphosphates in DNA replication by maintenance of the deoxynucleotide triphosphate (dNTP's and is known to be regulated by viral infection in the shrimp Litopenaeus vannamei. This paper describes the relationship between NDK and white spot syndrome virus (WSSV infection. The recombinant NDK was produced by a prokaryotic expression system. WSSV copy numbers and mRNA levels of IE1 and VP28 were significantly increased in shrimp injected with recombinant NDK at 72 h after WSSV infection. After synthesizing dsRNA-NDK and confirming the efficacy of NDK silencing, we recorded the cumulative mortality of WSSV-infected shrimp injected with NDK and dsRNA-NDK. A comparison between the results demonstrated that silencing NDK delayed the death of shrimps. These findings indicate that NDK has an important role influencing the replication of WSSV replication in shrimp. Furthermore, NDK may have potential target as a new therapeutic strategy against WSSV infection in shrimp.

  15. Litopenaeus vannamei hemocyanin exhibits antitumor activity in S180 mouse model in vivo.

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    Shangjie Liu

    Full Text Available Hemocyanin is a multifunctional glycoprotein, which also plays multiple roles in immune defense. While it has been demonstrated that hemocyanin from some mollusks can induce potent immune response and is therefore undergoing clinical trials to be used in anti-tumor immunotherapy, little is currently known about how hemocyanin from arthropods affect tumors. In this study we investigated the anti-tumor activity of hemocyanin from Litopenaeus vannamei on Sarcoma-180 (S180 tumor-bearing mice model. Eight days treatment with 4mg/kg bodyweight of hemocyanin significantly inhibited the growth of S180 up to 49% as compared to untreated. Similarly, histopathology analysis showed a significant decrease in tumor cell number and density in the tissues of treated mice. Moreover, there was a significant increase in immune organs index, lymphocyte proliferation, NK cell cytotoxic activity and serum TNF-α level, suggesting that hemocyanin could improve the immunity of the S180 tumor-bearing mice. Additionally, there was a significant increase in superoxide dismutase (SOD activity and a decrease in the level of malondialdehyde (MDA in serum and liver, which further suggest that hemocyanin improved the anti-oxidant ability of the S180 tumor-bearing mice. Collectively, our data demonstrated that L. vannamei hemocyanin had a significant antitumor activity in mice.

  16. A galectin from shrimp Litopenaeus vannamei is involved in immune recognition and bacteria phagocytosis.

    Science.gov (United States)

    Hou, Fujun; Liu, Yongjie; He, Shulin; Wang, Xianzong; Mao, Aitao; Liu, Zhigang; Sun, Chengbo; Liu, Xiaolin

    2015-06-01

    Galectins are conserved family members with β-galactosides affinity that play multiple functions in embryogenesis, development and regulation of innate and adaptive immunity. However, little functional studies were reported in crustaceans. Here, a shrimp Litopenaeus vannamei galectin (LvGal) cDNA was identified with an open reading frame of 1017 bp, which encodes a putative protein of 338 amino acids. A carbohydrate recognition domain (CRD) and several amino acids residues involved in dimerization were found in LvGal. LvGal mRNA was mainly expressed in gills and hemocytes and upregulated post Vibrio anguillarum challenge. Recombinant LvGal (rLvGal) was expressed in Escherichia coli BL21 (DE3) and the purified rLvGal could strongly bind G(-) bacteria V. anguillarum and G(+) bacteria Micrococcus lysodeikticus. Besides, rLvGal exhibited strong activity to agglutinate V. anguillarum and weak activity to agglutinate M. lysodeikticus but no obvious antibacterial activity was found with selected bacteria. In addition, in vivo experiments showed rLvGal could promote phagocytosis of bacteria by hemocytes. Thus, through these collective data we predicted LvGal is involved in immune recognition and functions as a potential pattern recognition receptor. Copyright © 2015 Elsevier Ltd. All rights reserved.

  17. Identification and characterization of two Croquemort homologues in penaeid shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Hou, Fujun; Liu, Tao; Wang, Qiai; Liu, Yongjie; Sun, Chengbo; Liu, Xiaolin

    2017-01-01

    Croquemort, the homologue of human CD36, is a member of class B scavenger receptors, which is involved in bacteria phagocytosis and cytokins release. However, there is still less information about Croquemort in crustaceans. Here, a Croquemort from Pacific white shrimp Litopenaeus vannamei (LvCroquemort) and its truncated form (LvCroquemort-S1) cDNA sequences were identified, characterized and their role in bacteria clearance was investigated. The deduced protein of LvCroquemort is 533 amino acids and contains typical domains of CD36: the N-terminus and C-terminus in cytoplasm, two transmembrane regions and a large extracellular loop-like domain. However, LvCroquemort-S1 losses partial cDNA sequence in its middle and its deduced protein losses the C-terminal transmembrane region and C-terminus in cytoplasm, the latter of which is found participating in cytokins release in human CD36. LvCroquemort transcript is highly expressed in gills, hemocytes, testis and slightly in heart, hepatopancreas and nerve. Besides, its responses to bacteria Vibrio anguillarum and white spot syndrome virus were examined. Knock-down of LvCroquemort by specific dsRNA reduces bacteria clearance. These initial data will help to further understand roles of Croquemort in crustacean innate immunity. Copyright © 2016 Elsevier Ltd. All rights reserved.

  18. Functional characterization of a reactive oxygen species modulator 1 gene in Litopenaeus vannamei.

    Science.gov (United States)

    He, Hong-Hui; Chi, Yi-Miao; Yuan, Kai; Li, Xiao-Yun; Weng, Shao-Ping; He, Jian-Guo; Chen, Yi-Hong

    2017-11-01

    Reactive oxygen species (ROS) imparts a dual effect on multicellular organisms, wherein high levels are usually harmful, and low levels could facilitate in combating pathogenic microorganisms; therefore, the regulation of ROS production is critical. Previous studies have suggested that ROS contributes to resistance to the white spot syndrome virus (WSSV) or Vibrio alginolyticus in Litopenaeus vannamei. However, the regulation of ROS metabolism in L. vannamei remains elusive. In the present study, we proved that the overexpression of L. vannamei reactive oxygen species modulator 1 (LvROMO1) increases ROS production in Drosophila Schneider 2 (S2) cells. Real-time RT-PCR analysis indicated that LvROMO1 is induced by WSSV or V. alginolyticus infection and β-glucan or microcystin (MC-LR) injection. Further investigation showed that LvROMO1 responding to MC-LR, thereby inducing hemocytes to undergo apoptosis, and ultimately resulting in hepatopancreatic damage. And LvROMO1 downregulation induced an increase in the cumulative mortality of WSSV-infected shrimp by reducing ROS production and suppressing the expression of antimicrobial peptides genes. The findings of present study suggest that LvROMO1 plays an important role in ROS production in L. vannamei and is involved in innate immunity. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Microbiological Investigation of Persistent Mortalities in Litopenaeus vannamei Grown in Low Saline Waters in India.

    Science.gov (United States)

    Sanathkumar, Hirekudel; Ravi, Charan; Padinhatupurayil, Suresh Babu; Mol, Mini; Prasad, Jilagam Krishna; Nayak, Binaya Bhusan

    2014-09-01

    Abstract Microbial diseases are a serious hindrance to successful shrimp aquaculture. The Pacific white shrimp Litopenaeus vannamei is an exotic species recently introduced in India to supplement the failing aquaculture of the Asian tiger shrimp Penaeus monodon due to viral diseases. However, after a brief initial success, the aquaculture of L. vannamei is also experiencing serious problems due to microbial diseases. In this study, we conducted a microbiological investigation into the problem of persistent mortalities in selected L. vannamei farms on the southeastern coast of India. The infected shrimps were positive for the white spot syndrome virus (WSSV) by a nested PCR, though no visible white spots were present on the animals. The shrimps were heavily colonized by Vibrio parahemolyticus, which were isolated from the hepatopancreas, gills, and the body surface. The pond water, despite being low saline groundwater, harbored large numbers of V. parahemolyticus and other Vibrio species, and V. parahemolyticus isolated from L. vannamei were resistant to β-lactam antibiotics and cephalosporins. Our results strongly suggest that the persistent mortalities of L. vannamei were due to a co-infection by V. parahemolyticus and WSSV. Received December 16, 2013; accepted February 14, 2014.

  20. Identification and functional characterization of a novel Spätzle gene in Litopenaeus vannamei.

    Science.gov (United States)

    Yuan, Kai; Yuan, Feng-Hua; Weng, Shao-Ping; He, Jian-Guo; Chen, Yi-Hong

    2017-03-01

    Shrimp innate immunity is the first line of resistance against pathogenic bacteria. The Toll-like receptor (TLR)-NF-κB pathway is vital in this immunity process. In this study, a novel Spätzle gene (LvSpz4) of Litopenaeus vannamei was cloned and functionally characterized. The open reading frame of LvSpz4 was 918 bp, which encoded a putative protein with 305 amino acids. LvSpz4 was most expressed in the gills of L. vannamei. This expression was induced by Vibrio alginolyticus or Staphylococcus aureus infection or lipopolysaccharide stimulation. The reporter gene assay showed that LvSpz4 could activate the promoters of Pen4, Drs, AttA, Mtk, and white spot syndrome virus immediate early gene1 in Drosophila Schneider 2 (S2) cells. Knockdown LvSpz4 increased the cumulative mortality of L. vannamei upon V. alginolyticus infection. The unfolded protein response (UPR) induced the expression of LvSpz4 in L. vannamei. Moreover, the promoter of LvSpz4 was activated by L. vannamei X-Box binding protein 1 and activating transcription factor 4 in S2 cells. These results suggested that LvSpz4 was involved in L. vannamei innate immunity and caused the crosstalk between the TLR-NF-κB pathway and UPR. Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. Effects of Copper on Hemocyte Apoptosis, ROS Production, and Gene Expression in White Shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Guo, Hui; Li, Kexu; Wang, Wei; Wang, Chenggui; Shen, Yuchun

    2017-10-01

    Copper, a common chemical contaminant in aquatic environment, is known to be toxic to aquatic life at high concentrations. In the present study, we evaluated the apoptotic cell ratio and ROS production in hemocytes of the white shrimp Litopenaeus vannamei exposed to 1 or 5 mg L-1 Cu for 0, 3, 6, 12, 24, and 48 h. The expression changes of antioxidant biomarker genes, i.e., copper-zinc superoxide dismutase (Cu-Zn SOD) and catalase (CAT), apoptosis-related genes, i.e., caspase-3 and inhibitor of apoptosis protein (IAP), and a specific biomarker gene of heavy metal pollution, i.e., metallothionein (MT), were also determined in hemocytes. Significant increases in ROS production were observed in both treatment groups at each time points. The apoptotic cell ratios were significantly increased at 6-48 h among shrimp exposed to 1 mg L-1 Cu and at each time points in 5 mg L-1 Cu group. These results indicated that Cu would induce oxidative stress and apoptosis in the hemocyte of L. vannamei. Quantitative real-time PCR analysis revealed that the relative expression levels of Cu-Zn SOD, CAT, caspase-3, IAP, and MT were upregulated in a dose-dependent and time-dependent manner, suggesting the involvement of these genes in stress response against Cu exposure.

  2. Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei

    Science.gov (United States)

    Yu, Yang; Zhang, Xiaojun; Liu, Jingwen; Li, Fuhua; Huang, Hao; Li, Yijun; Liu, Xiaolin; Xiang, Jianhai

    2015-01-01

    Selective breeding of the Pacific white shrimp Litopenaeus vannamei during the last decade has produced new varieties exhibiting high growth rates and disease resistance. However, the identification of new varieties of shrimps from their phenotypic characters is difficult. This study introduces a new approach for identifying varieties of shrimps using molecular markers of microsatellites and mitochondrial control region sequences. The method was employed to identify a new selected variety, Kehai No. 1 (KH-1), from three representative stocks (control group): Zhengda; Tongwei; and a stock collected from Fujian Province, which is now cultured in mainland China. By pooled genotyping of KH-1 and the control group, five microsatellites showing differences between KH-1 and the control group were screened out. Individual genotyping data confirmed the results from pooled genotyping. The genotyping data for the five microsatellites were applied to the assignment analysis of the KH-1 group and the control group using the partial Bayesian assignment method in GENECLASS2. By sequencing the mitochondrial control regions of individuals from the KH-1 and control group, four haplotypes were observed in the KH-1 group, whereas 14 haplotypes were obtained in the control group. By combining the microsatellite assignment analysis with mitochondrial control region analysis, the average accuracy of identification of individuals in the KH-1 group and control group reached 89%. The five selected microsatellite loci and mitochondrial control region sequences were highly polymorphic and could be used to distinguish new selected varieties of L. vannamei from other populations cultured in China.

  3. Effects of hypoxia on dopamine concentration and the immune response of White Shrimp ( Litopenaeus vannamei)

    Science.gov (United States)

    Hu, Fawen; Pan, Luqing; Jing, Futao

    2009-03-01

    Effects of hypoxia on the dopamine concentration and the immune response of White Shrimp Litopenaeus vannamei were studied. The results showed that hypoxia had significant effects on the concentration of dopamine (DA) in the haemolymph, haemocyte count, phenoloxidase activity, phagocytic activity of haemocytes and bacteriolytic and antibacterial activity in the haemolymph ( P<0.05). The concentration of the dopamine in haemolymph reached its maximum in the 3.0 and 1.5 mg L-1 DO groups at 12 h and 6 h, and then returned to normal after 24 h and 12 h, respectively. All immune parameters decreased with the reduction of dissolved oxygen. Total haemocyte count (THC), the hyaline cells and semi-granular cells in the 3.0 mg L-1 DO group became stable after 12 h, while granular cells did so after 24 h. The THC and different haemocyte count (DHC) in the 1.5 mg L-1 DO group became stable after 24 h. Phenoloxidase activity and bacteriolytic activity in the 3.0 and 1.5 mg L-1 DO groups reached their stable levels after 24 h and 12 h respectively, while phagocytic activity and antibacterial activity became stable after 24 and 12, and 36 and 24 h, respectively. It was also indicated that the changes of dopamine concentrations in haemolymph, haemocyte count and phenoloxidase activity were obviously related to the exposure time under hypoxic conditions.

  4. Silencing tyrosine hydroxylase retards depression of immunocompetence of Litopenaeus vannamei under hypothermal stress.

    Science.gov (United States)

    Mapanao, Ratchaneegorn; Chang, Chin-Chyuan; Cheng, Winton; Liu, Kuan-Fu

    2018-01-01

    Tyrosine hydroxylase (TH), the first and rate-limiting step in the synthesis of catecholamines, is required in catecholamine synthesis of the neuroendocrine regulatory network against stress in shrimp. The immunocompetence, catecholamine biosynthesis, and carbohydrate metabolites were evaluated in Litopenaeus vannamei received L. vannamei TH (LvTH) double-stranded (ds)RNA, diethyl pyrocarbonate-water, or non-targeted dsRNA for 3 days then transferred from 28 to 20 or 28 °C. The immunocompetence of LvTH-depleted shrimp held at 28 °C was promoted, and those were downregulated under hypothermal stress and revealed higher level than the other two dsRNA treatments. Meanwhile, the decrease of catecholamine biosynthesis was observed in LvTH-depleted shrimp held at 28 °C, and those were elevated under hypothermal stress and revealed lower levels, compared to two dsRNA treatments. The reduced carbohydrate metabolites was observed in LvTH-depleted shrimp held at 28 °C, and those were upregulated under hypothermal stress and showed lower levels than the other two dsRNA treatments. It was therefore concluded that LvTH-depleted shrimp revealed enhanced immunocompetence and reduced carbohydrate metabolites when exposed to a hypothermal stress condition, and in the meantime, even though catecholamine biosynthesis was downregulated, no significant difference was observed in DA or NE levels. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Immune gene expression for diverse haemocytes derived from pacific white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Yang, Chih-Chiu; Lu, Chung-Lun; Chen, Sherwin; Liao, Wen-Liang; Chen, Shiu-Nan

    2015-05-01

    In this study, diverse haemocytes from Pacific white shrimp Litopenaeus vannamei were spread by flow cytometer sorting system. Using the two commonly flow cytometric parameters FSC and SSC, the haemocytes could be divided into three populations. Microscopy observation of L. vannamei haemocytes in anticoagulant buffer revealed three morphologically distinct cell types designated as granular cell, hyaline cell and semigranular cell. Immune genes, which includes prophenoloxidase (proPO), lipopolysaccharide-β-glucan binding protein (LGBP), peroxinectin, crustin, lysozyme, penaeid-3a and transglutaminase (TGase), expressed from different haemocyte were analysed by quantitative real time PCR (qPCR). Results from the mRNA expression was estimated by relative level of each gene to β-actin gene. Finally, the seven genes could be grouped by their dominant expression sites. ProPO, LGBP and peroxinectin were highly expressed in granular cells, while LGBP, crustin, lysozyme and P-3a were highly expressed in semigranular cells and TGase was highly expressed in hyaline cells. In this study, L. vannamei haemocytes were firstly grouped into three different types and the immune related genes expression in grouped haemocytes were estimated. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. Actin genes and their expression in pacific white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Zhang, Xiaoxi; Zhang, Xiaojun; Yuan, Jianbo; Du, Jiangli; Li, Fuhua; Xiang, Jianhai

    2017-11-30

    Actin is a multi-functional gene family that can be divided into muscle-type actins and non-muscle-type actins. In this study, 37 unigenes encoding actins were identified from RNA-Seq data of Pacific white shrimp, Litopenaeus vannamei. According to phylogenetic analysis, four and three cDNAs belong to cytoplasmic- and heart-type actins and were named LvActinCT and LvActinHT, respectively. 10 cDNAs belong to the slow-type skeletal muscle actins, and 18 belong to the fast-type skeletal muscle actins; they were designated LvActinSSK and LvActinFSK, respectively. Some muscle actin genes formed gene clusters in the genome. Multiple alternative transcription starts sites (ATSSs) were found for LvActinCT1. Based on the early developmental expression profile, almost all LvActins were highly expressed between the early limb bud and post-larval stages. Using LvActinSSK5 as probes, slow-type muscle was localized in pleopod muscle and superficial ventral muscle. We also found three actin genes that were down-regulated in the hemocytes of white spot syndrome virus (WSSV)- and Vibrio parahaemolyticus-infected L. vannamei. This study provides valuable information on the actin gene structure of shrimp, furthers our understanding of the shrimp muscle system and helps us develop strategies for disease control and sustainable shrimp farming.

  7. Transcriptome analysis of the unfolded protein response in hemocytes of Litopenaeus vannamei.

    Science.gov (United States)

    Chen, Yi-Hong; Yuan, Feng-Hua; Bi, Hai-Tao; Zhang, Ze-Zhi; Yue, Hai-Tao; Yuan, Kai; Chen, Yong-Gui; Wen, Shao-Ping; He, Jian-Guo

    2016-07-01

    In this study, Litopenaeus vannamei was injected with double-stranded RNA (dsRNA) against L. vannamei immunoglobulin heavy chain binding protein (LvBip) to activating UPR in the hemocytes, shirmps injected dsRNA against enhanced green fluorescence protein (eGFP) as control group. And genes expression in hemocytes of then were analyzed using Illumina Hiseq 2500 (PE100). By comparing the analyzed results, 1418 unigenes were significantly upregulated, and 596 unigenes were significantly down-regulated upon UPR. Analysis of the differentially expressed genes against known databases indicated that the distribution of gene pathways between the upregulated and down-regulated genes were substantially different. A total of 208 genes of UPR system were obtained, and 69 of them were differentially expressed between the two groups. Results also showed that L. vannamei UPR was involved in various metabolic processes, such as glycometabolism, lipid metabolism, amino acid metabolism, and nucleic acid metabolism. In addition, UPR was emgaged in immune-assicoated signaling pathways, such as NF-κB signaling pathway, NOD-like receptor signaling pathway, Hippo signaling pathway, p38 MAPK signaling pathway and Wnt signaling pathway in L. vannamei. These results improved our current understanding of the L. vannamei UPR, and highlighted its importance in cell homeostasis upon environmental stress. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. Litopenaeus vannamei hemocyanin exhibits antitumor activity in S180 mouse model in vivo

    Science.gov (United States)

    Aweya, Jude Juventus; Zheng, Zhou; Zhong, Mingqi; Chen, Jiehui; Wang, Fan

    2017-01-01

    Hemocyanin is a multifunctional glycoprotein, which also plays multiple roles in immune defense. While it has been demonstrated that hemocyanin from some mollusks can induce potent immune response and is therefore undergoing clinical trials to be used in anti-tumor immunotherapy, little is currently known about how hemocyanin from arthropods affect tumors. In this study we investigated the anti-tumor activity of hemocyanin from Litopenaeus vannamei on Sarcoma-180 (S180) tumor-bearing mice model. Eight days treatment with 4mg/kg bodyweight of hemocyanin significantly inhibited the growth of S180 up to 49% as compared to untreated. Similarly, histopathology analysis showed a significant decrease in tumor cell number and density in the tissues of treated mice. Moreover, there was a significant increase in immune organs index, lymphocyte proliferation, NK cell cytotoxic activity and serum TNF-α level, suggesting that hemocyanin could improve the immunity of the S180 tumor-bearing mice. Additionally, there was a significant increase in superoxide dismutase (SOD) activity and a decrease in the level of malondialdehyde (MDA) in serum and liver, which further suggest that hemocyanin improved the anti-oxidant ability of the S180 tumor-bearing mice. Collectively, our data demonstrated that L. vannamei hemocyanin had a significant antitumor activity in mice. PMID:28854214

  9. Heritability of body weight and resistance to ammonia in the Pacific white shrimp Litopenaeus vannamei juveniles

    Science.gov (United States)

    Li, Wenjia; Lu, Xia; Luan, Sheng; Luo, Kun; Sui, Juan; Kong, Jie

    2016-09-01

    Ammonia, toxic to aquaculture organisms, represents a potential problem in aquaculture systems, and the situation is exacerbated in closed and intensive shrimp farming operations, expecially for Litopenaeus vannamei. Assessing the potential for the genetic improvement of resistance to ammonia in L. vannamei requires knowledge of the genetic parameters of this trait. The heritability of resistance to ammonia was estimated using two descriptors in the present study: the survival time (ST) and the survival status at half lethal time (SS50) for each individual under high ammonia challenge. The heritability of ST and SS50 were low (0.154 4±0.044 6 and 0.147 5±0.040 0, respectively), but they were both significantly different from zero ( P0.05), suggesting that ST and SS50 could be used as suitable indicators for resistance to ammonia. There were also positive phenotypic and genetic correlation between resistance to ammonia and body weight, which means that resistance to ammonia can be enhanced by the improvement of husbandry practices that increase the body weight. The results from the present study suggest that the selection for higher body weight does not have any negative consequences for resistance to ammonia. In addition to quantitative genetics, tools from molecular genetics can be applied to selective breeding programs to improve the efficiency of selection for traits with low heritability.

  10. Encapsulated Synbiotic Dietary Supplementation at Different Dosages to Prevent Vibriosis in White Shrimp, Litopenaeus vannamei

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    Anis Zubaidah

    2015-10-01

    Full Text Available The aim of this study was to evaluate the effect of encapsulated synbiotic (Bacillus sp. NP5 and oligosaccharide dietary at different dosages on growth performance, survival rate, feed conversion ratio, and immune responses of Litopenaeus vannamei against Vibrio infection. The shrimps of the main treatments were fed by the diet that contained three different dosages of encapsulated synbiotic [0.5% (A, 1% (B, and 2% (C (w/w] with feeding rate of 5% of shrimp biomass (4 times a day. The shrimps of two control treatments (negative control and positive control were fed only by commercial feed without supplementation of encapsulated synbiotic. The growth, feed conversion ratio, and survival rate were observed after 30 days of encapsulated synbiotic dietary. The shrimps were then challenged by injection of Vibrio harveyi (6 log colony forming units/mL 0.1 mL/shrimp, excluded the negative control treatment. Afterward, the survival and immune responses were observed for 9 days after experimental infection. The shrimps treated with 2% encapsulated synbiotic (treatment C in the diet showed the highest growth performance (2.98 ± 0.42%, feed conversion ratio (1.26 ± 0.19, and better immune responses i.e. total hemocyte counts, differential hemocyte count, phenoloxidase, and intestine bacteria observation compared to those of positive control treatment.

  11. EFEKTIVITAS PENGGUNAAN BAKTERI UNTUK PERBAIKAN KUALITAS AIR MEDIA BUDI DAYA UDANG VANAME (LITOPENAEUS VANNAMEI SUPER INTENSIF

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    Lufisari Herdianti

    2015-12-01

    Full Text Available Aquaculture activities in coastal area potentially produced wastes which leads water pollution. In order to reduce pollution, the use of several bacteria is very promising. This study was aimed to determine the effectivity of commercial bacterial inocculants to reduce pollution in cultured water. Bacterial inocculant tested in this study was SN®, SB®, and the combination of both inocculants for water quality improvements in white shrimp (Litopenaeus vannamei super intensive culture media. This study was conducting in two steps, namely (i preliminary study and (ii primary study. The preliminary study was aimed to determine the time required to add bacteria based on the total number of bacterial colonies. The result showed that total colonies number of media treated with addition of SN® and SB® were reached maximum within 2 days. The result of primary study showed that the combination of SN® and SB® showed best effect in maintaining water quality of the culture media. This combination are proven reduces ammonia by 96%, nitrite-N by 83%, and COD by 42%.

  12. Antibiotic resistance of Vibrio parahaemolyticus isolated from pond-reared Litopenaeus vannamei marketed in Natal, Brazil

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    Ligia Maria Rodrigues de Melo

    2011-12-01

    Full Text Available Ten out of fifty fresh and refrigerated samples of shrimp (Litopenaeus vannamei collected from retailers in Natal (Rio Grande do Norte, Northeastern Brazil tested positive for Vibrio parahaemolyticus. The Kanagawa test and multiplex PCR assays were used to detect TDH and TRH hemolysins and the tdh, trh and tlh genes, respectively. All strains were Kanagawa-negative and tlh-positive. Antibiotic susceptibility testing was done for seven antibiotics by the agar diffusion technique. Five strains (50% presented multiple antibiotic resistance to ampicillin (90% and amikacin (60%, while two strains (20% displayed intermediate-level resistance to amikacin. All strains were sensitive to chloramphenicol. Intermediate-level susceptibility and/or resistance to other antibiotics ranged from 10 to 90%, with emphasis on the observed growing intermediate-level resistance to ciprofloxacin. Half our isolates yielded a multiple antibiotic resistance index above 0.2 (range: 0.14-0.29, indicating a considerable risk of propagation of antibiotic resistance throughout the food chain.

  13. Responses of prophenoloxidase system and related defence parameters of Litopenaeus vannamei to low salinity

    Science.gov (United States)

    Pan, Luqing; Xie, Peng; Hu, Fawen

    2010-09-01

    In this study, we investigated the effects of low salinity (26 and 21) on the prophenoloxidase (proPO) system and related defence parameters in the shrimp Litopenaeus vannamei. The results showed that low salinity induced a significant increase of dopamine (DA) concentration in haemolymph at 6 h of the experiment; on the other hand, total haemocyte count (THC), differential haemocyte count (DHC) and PO activity decreased over time to the lowest level at 24 h and remained low thereafter. Serine Protease (SP) and Proteinase Inhibitor (PI) activity in the two lower salinity treatments decreased to the lowest level at 12 and 24 h, respectively, and both recovered to the control level at 72 h. In contrast, α2- macroglobulin (α2M) activity in the two lower salinity treatments peaked at 24 h and then decreased to the control level at 72 h. Therefore, it may be concluded that stress-induced DA plays an important temporary role in neurotransmission and causes immune response in L. vannamei in adapting to salinity changes.

  14. Effect of bioactive peptides (BPs) on the development of Pacific white shrimp ( Litopenaeus vannamei Boone, 1931)

    Science.gov (United States)

    Wang, Guangjun; Yu, Ermeng; Li, Zhifei; Yu, Deguang; Wang, Haiying; Gong, Wangbao

    2016-06-01

    The present study was conducted to evaluate the feasibility of replacing fish meal (FM) with bioactive peptides (BPs) in diet of white shrimp ( Litopenaeus vannamei). The changes in growth performance, body composition, non-specific immunity, and water quality were examined after the shrimp were fed four diets, in which 0% (control), 33.3%, 66.7% and 100% of FM was replaced by BPs, respectively. The groups were designated as Con, 1/3BPs, 2/3BPs, and 3/3BPs. A total of 720 shrimp with an initial body weight of 1.46 ± 0.78 g were fed the experimental diets for 56 days. The results revealed that: 1) the weight gain rate (WGR) in 1/3BPs, 2/3BPs, and 3/3BPs was significantly higher than that in Con ( P vannamei; it is able to effectively promote growth performance and improve immunity. Moreover, BPs in the diets had no negative impact on water quality.

  15. The detoxification process, bioaccumulation and damage effect in juvenile white shrimp Litopenaeus vannamei exposed to chrysene.

    Science.gov (United States)

    Ren, Xianyun; Pan, Luqing; Wang, Lin

    2015-04-01

    This study aimed to evaluate the effect of chrysene (CHR) on detoxification enzymes, bioaccumulation and effect of CHR on biomolecule damage in different organs of the juvenile white shrimp Litopenaeus vannamei. In this study, juvenile white shrimp L. vannamei were exposed to CHR for 21 days at four different concentrations as 0, 0.3, 2.1 and 14.7 μg/L. Results showed that CHR bioaccumulation increased rapidly at first then reached a plateau. The activities of aryl hydrocarbon hydroxylase (AHH), 7-ethoxyresorufin O-deethylase (EROD), epoxide hydrolase (EH), glutathione-S-transferase (GST), sulfotransferase (SULT) and uridinediphosphate glucuronyltransferase (UGT) were induced and then became stable gradually. Moreover, 2.1 and 14.7 μg/L CHR treatments increased activity of superoxide dismutase (SOD) in gills and hepatopancreas, while total antioxidant capacity (T-AOC) and GSH/GSSG were suppressed after CHR exposure. Additionally, lipid peroxidation (LPO) levels, protein carbonyl (PC) contents and DNA damage were induced throughout the exposure period, and different trends were detected with time of exposure. Overall, these novel findings of CHR bioaccumulation and resulted toxicity demonstrate that CHR could affect the physical status of L. vannamei. This study will form a solid basis for a realistic extrapolation scientific data for aquaculture water monitoring and food security. Copyright © 2015 Elsevier Inc. All rights reserved.

  16. Transcriptome Analysis of Litopenaeus vannamei in Response to White Spot Syndrome Virus Infection

    Science.gov (United States)

    Chen, Xiuli; Xie, Daxiang; Zhao, Yongzhen; Yang, Chunling; Li, Yongmei; Ma, Ning; Li, Ming; Yang, Qiong; Liao, Zhenping; Wang, Hui

    2013-01-01

    Pacific white shrimp (Litopenaeus vannamei) is the most extensively farmed crustacean species in the world. White spot syndrome virus (WSSV) is one of the major pathogens in the cultured shrimp. However, the molecular mechanisms of the host-virus interaction remain largely unknown. In this study, the impact of WSSV infection on host gene expression in the hepatopancreas of L. vannamei was investigated through the use of 454 pyrosequencing-based RNA-Seq of cDNA libraries developed from WSSV-challenged shrimp or normal controls. By comparing the two cDNA libraries, we show that 767 host genes are significantly up-regulated and 729 genes are significantly down-regulated by WSSV infection. KEGG analysis of the differentially expressed genes indicated that the distribution of gene pathways between the up- and down-regulated genes is quite different. Among the differentially expressed genes, several are found to be involved in various processes of animal defense against pathogens such as apoptosis, mitogen-activated protein kinase (MAPK) signaling, toll-like receptor (TLR) signaling, Wnt signaling and antigen processing and presentation pathways. The present study provides valuable information on differential expression of L. vannamei genes following WSSV infection and improves our current understanding of this host-virus interaction. In addition, the large number of transcripts obtained in this study provides a strong basis for future genomic research on shrimp. PMID:23991181

  17. Identification and functional characterization of a solute carrier family 15, member 4 gene in Litopenaeus vannamei.

    Science.gov (United States)

    Chen, Yong-Gui; Yuan, Kai; Zhang, Ze-Zhi; Yuan, Feng-Hua; Weng, Shao-Ping; Yue, Hai-Tao; He, Jian-Guo; Chen, Yi-Hong

    2016-04-01

    Innate immunity in shrimp is important in resisting bacterial infection. The NF-κB pathway is pivotal in such an immune response. This study cloned and functionally characterized the solute carrier family (SLC) 15 member A 4 (LvSLC15A4) gene in Litopenaeus vannamei. The open reading frame of LvSLC15A4 is 1, 902 bp long and encodes a putative 633-amino acid protein, which is localized in the plasma membrane and intracellular vesicular compartments. Results of the reporter gene assay showed that LvSLC15A4 upregulated NF-κB target genes, including the immediate-early gene 1 of white spot syndrome virus, as well as several antimicrobial peptide genes, such as pen4, CecA, AttA, and Mtk in S2 cells. Moreover, knocked-down expression of LvSLC15A4 reduced pen4 expression in L. vannamei. LvSLC15A4 down-regulation also increased the cumulative mortality of Vibrio parahemolyticus-infected L. vannamei. Furthermore, LvSLC15A4 expression was induced by unfolded protein response (UPR) in L. vannamei hematocytes. These results suggest that LvSLC15A4 participates in L. vannamei innate immunity via the NF-κB pathway and thus may be related to UPR. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. Analysis of genetic diversity and differentiation of seven stocks of Litopenaeus vannamei using microsatellite markers

    Science.gov (United States)

    Zhang, Kai; Wang, Weiji; Li, Weiya; Zhang, Quanqi; Kong, Jie

    2014-08-01

    Seven microsatellite markers were used to evaluate the genetic diversity and differentiation of seven stocks of Litopenaeus vannamei, which were introduced from Central and South America to China. All seven microsatellite loci were polymorphic, with polymorphism information content ( PIC) values ranging from 0.593 to 0.952. Totally 92 alleles were identified, and the number of alleles ( Na) and effective alleles ( Ne) varied between 4 and 21 and 2.7 and 14.6, respectively. Observed heterozygosity ( H o) values were lower than the expected heterozygosity ( H e) values (0.526-0.754), which indicated that the seven stocks possessed a rich genetic diversity. Thirty-seven tests were detected for reasonable significant deviation from Hardy-Weinberg equilibrium. F is values were positive at five loci, suggesting that there was a relatively high degree of inbreeding within stocks. Pairwise F st values ranged from 0.0225 to 0.151, and most of the stock pairs were moderately differentiated. Genetic distance and cluster analysis using UPGMA revealed a close genetic relationship of L. vannamei between Pop2 and Pop3. AMOVA indicated that the genetic variation among stocks (11.3%) was much lower than that within stocks (88.7%). Although the seven stocks had a certain degree of genetic differentiation and a rich genetic diversity, there is an increasing risk of decreased performance due to inbreeding in subsequent generations.

  19. Pulsed Ultraviolet Light Reduces Immunoglobulin E Binding to Atlantic White Shrimp (Litopenaeus setiferus Extract

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    Si-Yin Chung

    2011-06-01

    Full Text Available Pulsed ultraviolet light (PUV, a novel food processing and preservation technology, has been shown to reduce allergen levels in peanut and soybean samples. In this study, the efficacy of using PUV to reduce the reactivity of the major shrimp allergen, tropomyosin (36-kDa, and to attenuate immunoglobulin E (IgE binding to shrimp extract was examined. Atlantic white shrimp (Litopenaeus setiferus extract was treated with PUV (3 pulses/s, 10 cm from light source for 4 min. Tropomyosin was compared in the untreated, boiled, PUV-treated and [boiled+PUV]-treated samples, and changes in the tropomyosin levels were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE. IgE binding of the treated extract was analyzed via immunoblot and enzyme-linked immunosorbent assay (ELISA using pooled human plasma containing IgE antibodies against shrimp allergens. Results showed that levels of tropomyosin and IgE binding were reduced following PUV treatment. However, boiling increased IgE binding, while PUV treatment could offset the increased allergen reactivity caused by boiling. In conclusion, PUV treatment reduced the reactivity of the major shrimp allergen, tropomyosin, and decreased the IgE binding capacity of the shrimp extract.

  20. Pulsed ultraviolet light reduces immunoglobulin E binding to Atlantic white shrimp (Litopenaeus setiferus) extract.

    Science.gov (United States)

    Shriver, Sandra; Yang, Wade; Chung, Si-Yin; Percival, Susan

    2011-07-01

    Pulsed ultraviolet light (PUV), a novel food processing and preservation technology, has been shown to reduce allergen levels in peanut and soybean samples. In this study, the efficacy of using PUV to reduce the reactivity of the major shrimp allergen, tropomyosin (36-kDa), and to attenuate immunoglobulin E (IgE) binding to shrimp extract was examined. Atlantic white shrimp (Litopenaeus setiferus) extract was treated with PUV (3 pulses/s, 10 cm from light source) for 4 min. Tropomyosin was compared in the untreated, boiled, PUV-treated and [boiled+PUV]-treated samples, and changes in the tropomyosin levels were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). IgE binding of the treated extract was analyzed via immunoblot and enzyme-linked immunosorbent assay (ELISA) using pooled human plasma containing IgE antibodies against shrimp allergens. Results showed that levels of tropomyosin and IgE binding were reduced following PUV treatment. However, boiling increased IgE binding, while PUV treatment could offset the increased allergen reactivity caused by boiling. In conclusion, PUV treatment reduced the reactivity of the major shrimp allergen, tropomyosin, and decreased the IgE binding capacity of the shrimp extract.

  1. Transcriptomic response to low salinity stress in gills of the Pacific white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Hu, Dongxu; Pan, Luqing; Zhao, Qun; Ren, Qin

    2015-12-01

    The Pacific white shrimp, Litopenaeus vannamei (L. vannamei), is one of the most farmed species. Salinity is an important environmental factor that affects its growth and distribution. However, the molecular mechanism of the shrimp in response to salinity stress remains largely unclear. High-throughput sequencing is a helpful tool to analyze the molecular response to salinity challenge in shrimp. In the present study, the transcriptomic responses of the gills in L. vannamei under low salinity stress were detected by Illumina's digital gene expression system. A total of 10,725,789 and 10,827,411 reads were generated from the non-changed and low salinity changed groups, respectively. 64,590 Unigenes with an average length of 764 bp were generated. Compared with the control, 585 genes were differentially expressed under low salinity. GO functional analysis and KEGG pathway analysis indicated some vital genes in response to the challenge. Ten genes related to osmoregulation and ambient salinity adaption were selected to validate the DGE results by RT-qPCR. This work provides valuable information to study the mechanism of salinity adaption in L. vannamei. Genes and pathways from the results will be beneficial to reveal the molecular basis of osmoregulation. It also gives an insight into the response to the salinity challenge in L. vannamei. Copyright © 2015 Elsevier B.V. All rights reserved.

  2. Effect of salinity on regulation mechanism of neuroendocrine-immunoregulatory network in Litopenaeus vannamei.

    Science.gov (United States)

    Zhao, Qun; Pan, Luqing; Ren, Qin; Wang, Lin; Miao, Jingjing

    2016-02-01

    The effects of low salinity (transferred from 31‰ to 26‰, 21‰, and 16‰) on the regulation pathways of neuroendocrine-immunoregulatory network were investigated in Litopenaeus vannamei. The results showed that the hormones (corticotrophin-releasing hormone, adrenocorticotropic hormone) and biogenic amines (dopamine, noradrenaline, 5-hydroxytryptamine) concentrations in lower salinity groups increased significantly within 12 h. The gene expression of biogenic amine receptors showed that dopamine receptor D4 and α2 adrenergic receptor in lower salinity groups decreased significantly within 12 h, whereas the 5-HT7 receptor significantly increased within 1d. The second messenger synthetases (adenylyl cyclase, phospholipase C) and the second messengers (cyclic adenosine monophosphate, cyclic guanosine monophosphate) of lower salinity groups shared a similar trend in which adenylyl cyclase and cyclic adenosine monophosphate reached the maximum at 12 h, whereas phospholipase C and cyclic guanosine monophosphate reached the minimum. The immune parameters (total hemocyte count, phenoloxidase activity, phagocytic activity, crustin expression, antibacterial activity, C-type lectin expression, hemagglutinating activity) in lower salinity groups decreased significantly within 12 h. Except for the total hemocyte count, all the parameters recovered to the control levels afterwards. Therefore, it may be concluded that the neuroendocrine-immunoregulatory network plays a principal role in adapting to salinity changes as the main center for sensing the stress and causes immune response in L. vannamei. Copyright © 2016 Elsevier Ltd. All rights reserved.

  3. Antibiotic resistance of Vibrio parahaemolyticus isolated from pond-reared Litopenaeus vannamei marketed in natal, brazil

    Science.gov (United States)

    de Melo, Ligia Maria Rodrigues; Almeida, Dulce; Hofer, Ernesto; dos Reis, Cristhiane Moura Falavina; Theophilo, Grace Nazareth Diogo; Santos, André Felipe das Mercês; Vieira, Regine Helena Silva dos Fernandes

    2011-01-01

    Ten out of fifty fresh and refrigerated samples of shrimp (Litopenaeus vannamei) collected from retailers in Natal (Rio Grande do Norte, Northeastern Brazil) tested positive for Vibrio parahaemolyticus. The Kanagawa test and multiplex PCR assays were used to detect TDH and TRH hemolysins and the tdh, trh and tlh genes, respectively. All strains were Kanagawa-negative and tlh-positive. Antibiotic susceptibility testing was done for seven antibiotics by the agar diffusion technique. Five strains (50%) presented multiple antibiotic resistance to ampicillin (90%) and amikacin (60%), while two strains (20%) displayed intermediate-level resistance to amikacin. All strains were sensitive to chloramphenicol. Intermediate-level susceptibility and/or resistance to other antibiotics ranged from 10 to 90%, with emphasis on the observed growing intermediate-level resistance to ciprofloxacin. Half our isolates yielded a multiple antibiotic resistance index above 0.2 (range: 0.14–0.29), indicating a considerable risk of propagation of antibiotic resistance throughout the food chain. PMID:24031779

  4. Standardization of sodium metabisulfite solution concentrations and immersion time for farmed shrimp Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Luciana Trigueiro de Andrade

    2015-03-01

    Full Text Available Sodium metabisulfite is the main additive used in the prevention of melanosis in shrimp; however, it has currently been employed with great variation in concentration by producers. Thus, the aim of the present study was to determine the correlation between the concentration of the sodium metabisulfite solution and immersion time of the whole shrimp to obtain the concentration of sulfur dioxide (SO2 in the edible muscle of farmed shrimp (Litopenaeus vannamei in accordance with the limit established by law. For this, solutions of sodium metabisulfite at different concentrations (1%, 2 %, 3 %, 4% and 5% were prepared and samples of L. vannamei shrimp (100g were immersed during 10, 20 or 30 minutes at temperature of 7°C. For all treatment assayed the concentration of SO2 was determined in the edible muscle of farmed shrimp (L. vannamei. The results show that for the conditions used in this study, the correlations were linear, with significant increase (P<0.05 in the SO2 concentration in the edible muscle of shrimps both increasing sodium metabisulfite concentration as increasing immersion times, suggesting the immersion of shrimps in a 3% solution for a time of 13 minutes in order to obtain SO2 concentration of 100ppm in its edible muscle in accordance with Brazilian legislation

  5. Carotenoides totais em resíduos do camarão Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Larissa Mont'Alverne Jucá Seabra

    2014-02-01

    Full Text Available Considerando-se o possível aproveitamento dos resíduos, provenientes do beneficiamento do camarão, realizouse, neste trabalho, a determinação da concentração de carotenoides totais dos resíduos do camarão Litopenaeus vannamei e da farinha obtida após secagem. As análises foram realizadas nos resíduos in natura e na farinha recémprocessada (dia 0 e aos 60, 120 e 180 dias de armazenamento sob congelamento. Os resíduos frescos apresentaram, no dia 0, teores de 42,74 µg/g de carotenoides totais e, a farinha recém-processada, de 98,51 µg/g. Após 180 dias de armazenamento, sob congelamento, os teores de carotenoides totais diminuíram significativamente, quando comparados com os do dia 0 (p < 0,05.

  6. Rab from the white shrimp Litopenaeus vannamei: characterization and its regulation upon environmental stress.

    Science.gov (United States)

    Wang, Lei; Wang, Xiao-Rong; Liu, Jin; Chen, Chu-Xian; Liu, Yuan; Wang, Wei-Na

    2015-10-01

    With the destruction of the ecological environment, shrimp cultivation in China has been seriously affected by outbreaks of infectious diseases. Rab, which belong to small GTPase Ras superfamily, can regulate multiple steps in eukaryotic vesicle trafficking including vesicle budding, vesicle tethering, and membrane fusion. Knowledge of Rab in shrimp is essential to understanding regulation and detoxification mechanisms of environmental stress. In this study, we analyzed the functions of Rab from the Pacific white shrimp, Litopenaeus vannamei. Full-length cDNA of Rab was obtained, which was 751 bp long, with open reading frame encoding 206 amino acids. In this study, for the first time, the gene expression of Rab of L. vannamei was analyzed by quantitative real-time PCR after exposure to five kinds of environmental stresses (bacteria, pH, Cd, salinity and low temperature). The results demonstrate that Rab is sensitive and involved in bacteria, pH, and Cd stress responses and Rab is more sensitive to bacteria than other stresses. Therefore we infer that Rab may have relationship with the anti-stress mechanism induced by environment stress in shrimp and Rab could be used as critical biomarkers for environmental quality assessment.

  7. ACUICULTURA EPICONTINENTAL DEL CAMARÓN BLANCO DEL PACÍFICO, Litopenaeus vannamei (Boone, 1931.

    Directory of Open Access Journals (Sweden)

    Daniel Enrique Godínez Siordia

    2010-11-01

    Full Text Available Se hizo una revisión sobre el estatus del cultivo de camarón Litopenaeus vannamei en agua de baja salinidad, presentando características, ventajas, desventajas y medidas de mitigación sobre el impacto ambiental de este sistema de cultivo. Este trabajo aborda aspectos relevantes con la finalidad de trazar un camino hacia la sustentabilidad de esta alternativa de cultivo que se expande rápidamente en México.

  8. Oceanography and Water Quality Condition in Several Waters of Thousand Islands and Its Suitability for White Shrimp Litopenaeus Vannamei Culture

    OpenAIRE

    Effendi, Irzal; Suprayudi, Muhammad Agus; Nurjaya, I Wayan; Surawidjaja, Enang Harris; Supriyono, Eddy; Junior, Muhammad Zairin; Sukenda .

    2016-01-01

    The purposes of this study were to determine oceanographic and water quality parameters and their suitability for white shrimp Litopenaeus vannamei culture. The measurements were carried out on dry season in Semak Daun Island, Karya Island, and Panggang Island waters of Thousand Islands, with areas of 315.0, 12.0, and 102.8 ha, water depth average of 4.6 m (0.5-28.1 m), 14.6 m (0.5-26.7 m), and 5.3 m (0.8-13.6 m), mean current water velocity of 12.9, 12.7, and 13.5 cm/second, respectively. ...

  9. Production and valuation of foods for animal monogástricos, with biological silage of remains of the prawn prosecution (Litopenaeus vannamei) fermented with lactobacillus

    OpenAIRE

    Sánchez, Héctor; Ochoa, Gloria

    2016-01-01

    This study allowed the valuation of diets using biological silage of residuals of the prosecution of (Litopenaeus vannamei) EB, fermented inoculum of commercial organisms (Lactobacillos acidofilos) or isolated from the digestive tract of the pig (Enterococcus hirae, Lactobacillus brevis, Lactobacillus johnsonii, Pediococcus pentosaceus) single use in piglets; EB preparation, cooking residues, milling, mixing (molasses and inoculum) and incubation; diet formulation according sp. and physiologi...

  10. Effects of dietary supplementation of a commercial prebiotic on survival, growth, immune responses, and gut microbiota of the Pacific white shrimp, Litopenaeus vannamei

    Science.gov (United States)

    A 35-day feeding trial was conducted to evaluate growth, bacterial populations of the gastrointestinal tract (GIT), and immune responses of Litopenaeus vannamei fed diets containing the commercial prebiotic PrevidaTM. Four diets were formulated to contain Previda at 0, 0.2, 0.5, 1.0, or 1.6% by wei...

  11. Effects of dietary supplementation of a commercial prebiotic Previda on survival, growth, immune responses and gut microbiota of pacific white shrimp, Litopenaeus vannamei

    Science.gov (United States)

    A 35-day feeding trial was conducted to evaluate growth, bacterial populations of the gastrointestinal tract (GIT), and immune responses of Litopenaeus vannamei fed diets containing the commercial prebiotic PrevidaTM. Four diets were formulated to contain Previda at 0, 0.2, 0.5, 1.0, or 1.6% by we...

  12. Size-composition of Annual Landings in the White Shrimp, Litopenaeus setiferus, Fishery of the Northern Gulf of Mexico, 1960-2006: Its Trend and Relationships with Other Fishery-dependent Variables

    National Research Council Canada - National Science Library

    Nance, James M; Caillouet, Charles W., Jr; Hart, Rick A

    2010-01-01

    The potential for growth overfishing in the white shrimp, Litopenaeus setiferus, fishery of the northern Gulf of Mexico appears to have been of limited concern to Federal or state shrimp management...

  13. LA VIDA SILVESTRE MANEJADA COMO RECURSOS DE USO COMÚN: ESTUDIO DE CASO EN MÉXICO

    Directory of Open Access Journals (Sweden)

    Alejandra Juárez Mondragón

    2015-12-01

    Full Text Available Desde 1997, el ejido Ajuchitlán Santiopan implementó reglas de aprovechamiento del venado cola blanca (Odocoileus virginianus mexicanus. Paralelamente registró una Unidad de Manejo para la Conservación de la Vida Silvestre (UMA, pretendiendo una caza sustentable. Esto implicó un co-manejo entre regulación local y federal. En México existe insuficiente conocimiento de estos procesos y de las repercusiones que generan en los ejidos. El objetivo del estudio fue comprender (desde la percepción de los pobladores la relación entre la creación de la institución local y el establecimiento de la UMA, utilizando la perspectiva teórica de recursos de uso común (RUC. Se realizaron entrevistas, listados libres, observación participante y consulta documental. De los entrevistados, el 46 % consideró que algunas leyes federales afectan tradiciones, y mencionaron que el 20 % dejó de respetar reglas ejidales. Sin embargo, 78 % reconoció importante mantener la UMA, porque: genera empleos temporales, incrementó la abundancia de venados y suscitó la reintroducción natural de especies ya extintas en el ejido. El 100 % coincidió en que la UMA es el factor principal de conservación de especies silvestres. Para continuar con este proceso es esencial replantear colectivamente objetivos de aprovechamiento y estrategias de vigilancia, ante las condiciones actuales.   White-tailed deer (Odocoileus virginianus mexicanus use regulations were implemented by the ejido Ajuchitlán Santiopan, since 1997. In parallel, the ejidatarios registered a Management Unit for the Conservation of Wildlife (UMA, seeking its sustainable hunting. This involved a co-management between local and federal regulation. The knowledge on these types of processes is sparse in Mexico, and of the impact that they generate in the ejidos. The aim of this study was to understand (from the perception of the inhabitants the relationship

  14. Estudio de la resistencia a Bemisia tabaci (Gennadius (Hemiptera: Aleyrodidae en germoplasma cultivado y silvestre de tomate (Solanum lycopersicum L.

    Directory of Open Access Journals (Sweden)

    Yeimy García Valencia

    2013-12-01

    Full Text Available En la Universidad Nacional de Colombia sede Palmira, en condiciones de campo y casa de malla, se evaluaron por preferencia para oviposición y número de ninfas de mosca blanca (Bemisia tabaci (Gennadius varias accesiones silvestres y poblaciones segregantes de tomate resistentes al pasador del fruto, Neoleucinodes elegantalis (Guenée. Se utilizó un diseño de bloques completos al azar con cuatro repeticiones, donde se evaluaron como variables el número de huevos y de ninfas del insecto por hoja. En cada observación se incluyeron tres hojas del tercio superior, medio e inferior del tallo de la planta. Las poblaciones de mosca blanca fueron muy bajas en los dos primeros ciclos de cultivo (en campo debido, probablemente, a las altas precipitaciones. Estas condiciones no permitieron una selección de materiales resistentes o susceptibles a este insecto. En el tercer ciclo de cultivo, bajo condiciones de casa de malla, no se encontraron diferencias significativas entre los retrocruzamientos respecto al testigo susceptible para la variable preferencia por oviposición. El genotipo 4212P4 presentó el menor número de ninfas, mostrando que tiene posiblemente un mecanismo que le confiere menor atracción para B. tabaci. Se encontró que los genotipos menos preferidos para oviposición y con menor número de ninfas fueron PI 134417, PI 134418 y PI 126449 (Solanum habrochaites var. Glabratum S. Knapp y D. M Spooneer.

  15. Sistemas de transmisión del conocimiento etnobotánico de plantas silvestres comestibles en Turbo, Antioquia, Colombia.

    Directory of Open Access Journals (Sweden)

    Ramón Antonio Mosquera Mena

    2015-03-01

    Full Text Available Los diferentes estudios etnobotánicos y etnoecológicos, muestran no solo la gran riqueza de recursos naturales que muchas comunidades han aprovechado de manera sostenible en el tiempo, si no también, la preocupación por la falta de sistematización del conocimiento debido a diferentes situaciones que lo dificultan. Esta investigación tiene como propósito mostrar los diferentes sistemas de transmisión del conocimiento que los habitantes del municipio de Turbo, Antioquia, Colombia, han utilizado para que las generaciones presentes y futuras reconozcan las plantas silvestres comestibles, lo cual es útil para tomar decisiones frente a la necesidad de sistematización del conocimiento de la biodiversidad local. Se utiliza una metodología que comprende las etapas de diseño de la encuesta etnobotánica, la aplicación de la misma teniendo en cuenta el tamaño de la población lo que arroja un total de 360 encuestas aplicadas en los 18 corregimientos del municipio, agrupados en cuatro sectores fisiográficos: Serranía, Litoral, Abanico Aluvial y Planicie Inundable. Los resultados muestran las formas de transmisión de conocimiento en cada uno de los corregimientos que componen la geografía del territorio, evidenciando que continúan primando sistemas de transmisión oral con muy baja sistematización y con un desinterés de la población joven por reconocer dichos recursos orientados a la preservación como fuentede alimento.

  16. Fauna silvestre asociada a ganado vacuno doble propósito en sistema de silvopastoreo, Pinto, Magdalena, Colombia

    Directory of Open Access Journals (Sweden)

    Jaime De La Ossa V.

    2011-12-01

    Full Text Available Objetivo. Evaluar la biodiversidad faunística asociada a ganadería vacuna de doble propósito en dos potreros estructurados con Botriochloa pertusa (colosoana como herbácea dominante, uno de ellos plantado con Crescentia cujete (Bignoniaceae como fuente de alimento adicional y otro con escaso y disperso arbolado. Materiales y métodos. El área de estudio está ubicada en el municipio de Pinto, Magdalena. Cada potrero evaluado fue de 10 ha, se mantuvo en cada uno un lote de quince vacas (½ Cebú, ¼ Pardo y ¼ Costeño con Cuernos, la separación entre las áreas muestreadas fue de 500m. Los registros de fauna se condujeron mediante punto fijo de conteo, en diez sitios de avistamiento, durante 20 días continuos, con cubrimiento visual amplio. Al ganado vacuno adulto presente en las dos áreas de trabajo se le realizó un pesaje individual al inicio del trabajo y al final del mismo, con el fin de determinar posibles diferencias entre ellos. Resultados. Estadísticamente el número de individuos y el número de especies presentaron diferencias significativas, siendo mayor la diversidad biológica para el potrero que poseía la plantación de C. cujete, mientras que el peso corporal de las vacas sometidas a pastoreo en las dos áreas no presentó diferencias significativas. Conclusiones. Se demuestra la importancia de este modelo pecuario como promotor de la conservación faunística local al convertirse enrefugio de variadas especies silvestres, al tiempo que mantiene la productividad existente sin alterar el ambiente sustancialmente.

  17. ANÁLISIS DEL MARCO REGULATORIO EN EL APROVECHAMIENTO DE LOS HONGOS SILVESTRES COMESTIBLES EN MÉXICO

    Directory of Open Access Journals (Sweden)

    Griselda Benítez-Badillo

    2013-01-01

    Full Text Available La recolección y venta de hongos silvestres comestibles, junto con la de otros productos forestales no maderables, constituyen una estrategia alimentaria y de subsistencia para los productores rurales. A pesar de la importancia social, económica, ecológica y cultural, dichas actividades todavía son de las más aisladas y poco conocidas del sector primario nacional. Esto trae como consecuencia la ausencia de políticas, esquemas de apoyo y estrategias de desarrollo para tales actividades. Por lo anterior, el objetivo de este trabajo fue exponer la necesidad de generar estadísticas oficiales en la recolección de hongos y establecer la relación entre la investigación científica, políticas públicas y el marco legal vigente, con el fin de conocer el estado actual de esta actividad y sus perspectivas a futuro. Se observa que el desconocimiento de la recolección de hongos ocasiona que no se le considere y excluya de las políticas de desarrollo del gobierno y de los sectores social y privado. De la misma manera, la falta de información, gestión y vinculación causa el rezago de la recolección y, con ello, su eventual sustitución o reemplazo por otras actividades. Sin embargo, existen ciertas potencialidades que debidamente manejadas pueden representar una alternativa real en el manejo sustentable de los bosques de México.

  18. Prácticas culinarias y (reconocimiento de la diversidad local de verduras silvestres en el Colectivo Mujeres y Maíz de Teopisca, Chiapas, México

    Directory of Open Access Journals (Sweden)

    Celina Guadalupe Solís Becerra

    2014-07-01

    Full Text Available Se analizan los saberes y acciones relacionados con la diversidad local de verduras silvestres, reconocida por las integrantes del Colectivo Mujeres y Maíz de Teopisca, Chiapas, México, a partir de sus prácticas culinarias. Se documentaron trece especies de verduras silvestres mayormente presentes en traspatios y 33 recetas culinarias con incorporación de tales especies. Se destaca la importancia de las verduras silvestres, la gastronomía local, el trabajo cotidiano y la vida campesina, con los cuales se enriquece la relación que las mujeres del colectivo y sus familias tienen con la biodiversidad local a través de su cultura alimentaria.

  19. Biossegurança no manejo de animais silvestres em áreas de confinamento no Estado do Piauí

    Directory of Open Access Journals (Sweden)

    Felipe José Costa Viana

    2016-02-01

    Full Text Available A biossegurança em áreas de confinamento de animais silvestres é questão fundamental para a saúde pública, sobretudo em locais onde os animais são considerados protagonistas da atração, como em parques zoológicos. Estes locais, apesar de toda segurança envolvida na contenção e manejo dos animais, ainda podem ser considerados como ponto de transmissão de zoonoses. Objetivou-se avaliar o grau de insalubridade dos funcionários e o uso de equipamentos de proteção contra patógenos de caráter zoonótico em áreas de confinamento de animais silvestres. O estudo foi realizado através da análise dos dados obtidos por meio de questionário aplicado aos trabalhadores de áreas de confinamento de animais silvestres, cujo enfoque era sobre a rotina de trabalho, manejo dos animais, histórico de saúde e o conhecimento acerca de doenças zoonóticas. As informações coletadas revelaram que, em parte, a administração do local proporciona aos funcionários uma segurança básica, fornecendo EPIs e cursos de atualização no manejo dos animais. Entretanto, a vestimenta utilizada pelos funcionários é lavada em suas próprias residências. Ainda há pontos a serem corrigidos na biossegurança nestes parques, como a construção de vestiários e uniformes próprios, com a lavagem do mesmo em um setor de lavanderia no próprio local.

  20. Antelo, Estanislao, Pedagogías silvestres: los caminos de la formación : Editorial Arandu, Goya, 2015

    OpenAIRE

    Guardabassi, Francisco José

    2016-01-01

    En el libro Pedagogías Silvestres: caminos de la formación, Estanislao Antelo nos presenta una vasta serie de entrevistas a profesores que desempeñan la docencia en asignaturas diversas, como la filosofía, la medicina, la danza, el canto o artes plásticas y que comparten la característica de no haber transitado por ninguna formación pedagógica específica y de sentirse orgullosos de sus trabajos. Ante ellos se despliegan una serie de indagaciones sobre su historia personal y formativa, que con...

  1. HONGOS COMESTIBLES SILVESTRES: ESPECIES EXÓTICAS DE SUILLUS (BOLETALES, BASIDIOMYCOTA Y LACTARIUS (RUSSULALES, BASIDIOMYCOTA ASOCIADAS A CULTIVOS DE PINUS ELLIOTTII DEL NORDESTE ARGENTINO

    Directory of Open Access Journals (Sweden)

    Nicolás Niveiro

    2009-01-01

    Full Text Available Los hongos silvestres comestibles son un recurso económico importante que permite obtenerun producto deshidratado que puede comercializarse fácilmente. En el presente trabajo, y conel objeto de dar a conocer este recurso, se ilustran y describen dos especies de hongosmicorrícicos asociadas a cultivos de Pinus elliottii en el nordeste argentino que no han sidomencionadas anteriormente para la región: Lactarius deliciosus para la provincia de Misionesy Suillus granulatus para las provincias de Corrientes y Misiones

  2. Diagnóstico del tráfico ilegal y del manejo post decomiso de fauna silvestre en nueve corporaciones autónomas regionales de Colombia

    Directory of Open Access Journals (Sweden)

    Montenefgro Olga Lucía

    2007-12-01

    Full Text Available La fauna silvestre siempre ha estado relacionada con el hombre sirviéndole de diferentes maneras, ya sea como fuente de alimento, como compañía, como materia prima para la fabricación de accesorios (pieles y otros productos o como diversión. Una de las causas más importantes de pérdida de diversidad la constituye el aprovechamiento no sostenible de especies de fauna y flora tanto para el consumo directo como para el comercio, lo que conlleva a la reducción en el tamaño de las poblaciones silvestres y por tanto una mayor vulnerabilidad a la extinción. El tráfico ilegal de especies silvestres es considerado a nivel mundial como una de las principales causas de disminución de las poblaciones naturales y como uno de los mercados ilegales que mayores sumas de dinero moviliza anualmente, con el fin de surtir la demanda de los mercados mundiales de la moda, las excentricidades de pequeños grupos, las investigaciones biomédicas y comportamentales, la novedad causada a turistas y las prácticas culturales o religiosas de muchas poblaciones humanas. En Colombia uno de los problemas de conservación más complejos lo representa el manejo de aquellos especímenes de fauna silvestre que son objeto de decomiso por parte de las autoridades ambientales regionales. Para lograr una adecuada disposición de la gran cantidad de animales silvestres confiscados o entregados voluntariamente en Colombia, el Ministerio del Medio Ambiente y las demás autoridades ambientales acordaron poner en marcha nueve centros regionales para el manejo de fauna decomisada o
    Centros de Atención y Valoración (CAV. Esta disposición tenía el propósito de implementar parámetros y protocolos que faciliten tomar decisiones que tanto las instituciones como los profesionales encargados enfrentan a diario. Sin embargo, el manejo de la fauna postdecomiso continua siendo uno de los problemas más complejos debido a la gran cantidad de animales decomisados y a la poca

  3. Dieta del perro (canis familiaris) y sus interacciones con la fauna silvestre de la cuenca alta del Río Otún-Risaralda (Colombia)

    OpenAIRE

    Manjarrés Rodríguez, Tania Solanye

    2015-01-01

    El perro (Canis familiaris) interactúa con la fauna silvestre principalmente por medio de la depredación, competencia y transmisión de enfermedades. Sin embargo, no se conoce con mucha claridad cómo se presentan estas interacciones. Para responder a este interrogante, en este trabajo se identificó la dieta del perro y la presencia de la enfermedad de Parvovirus (CPV). A partir de esta información, contrastada con el conocimiento de los pobladores del área de estudio, se determinaron las posib...

  4. Ocurrencia de Orthopoxvirus en Ardillas Silvestres (Sciuridae) y su Introducción a Nuevas Áreas Geográficas

    OpenAIRE

    Laura Jaramillo-Ortiz; Diego Soler-Tovar

    2015-01-01

    Orthopoxvirus (Familia Poxviridae) es un virus DNA causante de diversas enfermedades zoonóticas que involucran a mamíferos silvestres y domésticos, teniendo como reservorio principal los roedores. Las ardillas son uno de los roedores huéspedes de Orthopoxvirus; sin embargo, su comportamiento es variable de acuerdo a su distribución geográfica. En Norteamérica, el virus es endémico y poco patógeno en ardillas grises (Sciurus carolinensis); de otro lado, en Italia, República de Irlanda y el Rei...

  5. Musculatura da asa da arara canindé (Ara ararauna, Linnaeus, 1758): conhecimento básico aplicado à clínica de animais silvestres

    OpenAIRE

    Kamal Achôa Filho; Rafael Magdanelo Leandro; Marcio Nogueira Rodrigues; Erika Toledo da Fonseca; Vanessa Bertagia Pasqualetti; Maria Angélica Miglino

    2014-01-01

    http://dx.doi.org/10.5007/2175-7925.2014v27n2p137 Este trabalho teve o objetivo de descrever a musculatura das asas da arara canindé (Ara ararauna), visando a contribuir para aumentar o conhecimento acerca de sua anatomia e auxiliar a prática clínica com essa espécie. Foram utilizadas cinco araras provenientes do Centro de Triagem de Animais Silvestres da Paraíba (Cetas-PB), sendo a musculatura identificada mediante dissecação direta. Os músculos flexores, assim como os extensores, mostra...

  6. Priorización de enfermedades virales zoonóticas en la interfaz de cerdos silvestres, cerdos domésticos y seres humanos

    Directory of Open Access Journals (Sweden)

    Diana Benavides-Arias

    2016-08-01

    Conclusión. Los métodos semicuantitativos de priorización son una fuente de información para la toma de decisiones, pero su utilización es poco frecuente en los países en desarrollo por la falta de datos de vigilancia en salud pública. El control de las enfermedades que afectan tanto a los seres humanos como a los animales silvestres, requiere el desarrollo de estrategias que reduzcan la transmisión de patógenos de estos a los animales domésticos y a los seres humanos.

  7. Efecto del fotoperiodo y la temperatura sobre la composición bioquímica en reproductores silvestres de cabrilla sardinera, Mycteroperca rosacea (Streets, 1877)

    OpenAIRE

    José Antonio Estrada-Godínez; Minerva Maldonado-García; Vicente Gracia-López; Manuel Carrillo; René Rebollar-Prudente; Milton Spanopoulos-Zarco

    2014-01-01

    Se estimó el factor de condición (K), el índice gonadosomático (IGS), hepatosomático (IHS) y de grasa visceral (IGV), así como la composición bioquímica en diferentes tejidos de reproductores silvestres de cabrilla sardinera, Mycteroperca rosacea, para evaluarlos a lo largo de un ciclo reproductivo y ver la relación que presentan los cambios con respecto a la temperatura del agua y fotoperiodo de la zona de muestreo. Se capturaron 187 reproductores, 146 hembras y 41 machos en el golfo de Cali...

  8. Diagnóstico del tráfico ilegal y del manejo post decomiso de fauna silvestre en nueve corporaciones autónomas regionales de Colombia

    OpenAIRE

    Montenefgro Olga Lucía; Mendivelso Gamboa Diego Andrés

    2007-01-01

    La fauna silvestre siempre ha estado relacionada con el hombre sirviéndole de diferentes maneras, ya sea como fuente de alimento, como compañía, como materia prima para la fabricación de accesorios (pieles y otros productos) o como diversión. Una de las causas más importantes de pérdida de diversidad la constituye el aprovechamiento no sostenible de especies de fauna y flora tanto para el consumo directo como para el comercio, lo que conlleva a la reducción en el tamaño de las poblaciones sil...

  9. Revisión del concepto biológico desarrollado en la legislación colombiana relacionada con la fauna silvestre vertebrada terrestre

    Directory of Open Access Journals (Sweden)

    Maldonado Chaparro Adriana A.

    2004-12-01

    Full Text Available En el trabajo se presenta un panorama general del estado actual de la legislación en materia de fauna silvestre vertebrada terrestre en Colombia, el cual es complementado con algunas actividades de conservación y aprovechamiento del recurso faunístico, que han sido adelantadas por
    las autoridades ambientales. Además, se exponen y analizan conceptos biológicos usados dentro de la normatividad, que son considerados claves desde el punto de vista biológico para la aplicación de las normas.

  10. DETECCIÓN DE ANTICUERPOS CONTRA EL VIRUS DE LA ENFERMEDAD DE NEWCASTLE EN AVES SILVESTRES PASERIFORMES Y COLUMBIFORMES EN LA PROVINCIA DE CHANCAY.

    OpenAIRE

    Chang B., Pablo; Práctica privada; Icochea D´A., Eliana; Laboratorio de Patología Aviar, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima.; Alba Ch., Monica; Laboratorio de Patología Aviar, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima.

    2014-01-01

    Se utilizó la prueba de inhibición de la hemaglutinación para determinar la prevalencia de anticuerpos contra el virus de la enfermedad de Newcastle en aves cantoras silvestres sanos (Paseriformes) (N=263), las tórtolas y las palomas (Columbiformes) (n=l35) de la provincia de Chancay, Perú. Todos los animales sometidos a pruebas fueron negativos, lo que sugiere que las aves no eran expuestos al virus o que la prevalencia es inferior a 0,75%.

  11. Intoxicación por munición de plomo en aves silvestres en España y medidas para reducir el riesgo

    OpenAIRE

    Mateo, R.; N. Vallverdú-Coll; M.E. Ortiz-Santaliestra

    2013-01-01

    La intoxicación por ingestión de munición de plomo en aves silvestres es conocida en España desde finales del siglo pasado. Elevadas densidades de perdigones de plomo en diversos humedales de importancia internacional producen tasas de ingestión de perdigones cercanas al 70 % en es- pecies como el ánade rabudo (Anas acuta) y el plumbismo llega a ser una de las principales causas de mortalidad de la amenazada malvasía ca- beciblanca (Oxyura leucocephala). El plumbismo también ha sido descrito ...

  12. Técnica de Necropsia, Interpretación de Hallazgos Macroscópicos y Toma de Muestras en Mamíferos Silvestres

    OpenAIRE

    Jahnier Caicedo; Ospina, J. C.; Avila, J

    2012-01-01

    La correcta realización de necropsias y toma de muestras en los animales silvestres de los diferentes sistemas de conservación del país es una herramienta de alta importancia en el manejo epidemiológico de muchas enfermedades. La falta de entrenamiento en las técnicas de necropsia de estos animales dificulta la elaboración de propuestas diagnósticas y de control que repercutan en los problemas sanitarios; por lo cual, se presenta una guía de técnica de necropsia general para mamíferos silvest...

  13. The effect of phytase levels in the diet on the digestibility and growth performance of white shrimp Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Muhammmad Agus Suprayudi

    2013-11-01

    Full Text Available This experiment was conducted to evaluate the effect of phytase levels in the diet on the digestibility and growth performance of white shrimp Litopenaeus vanname. White shrimp were used in the trial with initial body weight of 3.8±0.01 g were reared in  aquarium (60×40×50 cm3 and filled with 60L treated sea water at a density of ten individu. Experimental diet were formulated in isonitrogeneous (36% protein and isocaloric (3960 kcal/kg to contain four levels of phytase: 0 iu/kg (diet A, 500 iu/kg (diet B, 750 iu/kg (diet C, and 1000 iu/kg(diet D. All diets were supplied with 0.5% of Cr2O3 as an indicator for digestibility measurement shrimp fed three times daily at satiation levelfor how many days of rearing. A factorial completely randomized experimental design was selected consisted of four treatments and triplicates. The result showed that the phytase addition improved the digestibility of protein, calcium, and phosphor as well as improve growth performance of white shrimp. Based on evaluation of those parameters, it is concluded that phytase level of500 IU support the best growth performance ofjuvenile white shrimp. Keywords: phytase, feed digestibility, growth,Litopenaeus vannamei

  14. FONTES E NÍVEIS DE VITAMINA C PARA O CAMARÃO Litopenaeus vannamei NA FASE DE RECRIA

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    Neyva Ribeiro Ferraz

    2012-09-01

    Full Text Available The inclusion of different sources and levels of vitamin C was evaluated on growth, survival, protein retention and health of Litopenaeus vannamei with initial weight of 5.90 ± 0.57 g. Five hundred and forty individuals were distributed in 27 cages in a completely randomized design in a 4x2 factorial scheme (four sources and two levels (n = 3. The sources used were ascorbic acid crystal, coated ascorbic acid, ascorbic acid monophosphate and ascorbic acid PEG (polyethylene glycol with two levels: 180 and 260 mg kg-1. No influence was observed (P>0.05 from the source, level of vitamin C nor interaction of these factors on growth, survival, retention of protein and parameters of health of the shrimp. Necrosis was observed in the abdomen of all animals. The use of vitamin C in sources and levels estimated for the shrimp Litopenaeus vannamei weighing over 5 g shows no improvement in growth, survival, protein retention and health of shrimp.

  15. La investigación en torno a la concepción de vida silvestre: Una aproximación al estado del arte en el contexto educativo.Pág 51-87

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    Luis Juan Rubiano O.

    2011-07-01

    Full Text Available Se hace una revisión sobre el origen de la concepción de vida silvestre, rastreando su significado desde el siglo XIV hasta el presente. Aunque existen en Inglaterra referencias muy antiguas sobre la concepción de vida silvestre, es en Estados Unidos donde nace y evoluciona dicho término. Al comparar las concepciones de la vida silvestre en los Estados Unidos respecto a los países latinoamericanos, se evidencia la enorme importancia cultural, educativa, política y social dada a tales concepciones en el continente norteamericano en contraste con Latinoamérica, particularmente Colombia, en donde no se dan tales manifestaciones. Igualmente, la historia de la educación e investigación en educación sobre la vida silvestre en los Estados Unidos revela una notoria inserción de esta temática tanto en los textos divulgativos (educación no formal como en la literatura científica y en los currículos de educación básica y universitaria. En dicho país es posible conjeturar un cambio evolutivo en la concepción de vida silvestre que, de acuerdo con nuestra investigación, se inicia a finales del siglo XIX con un significado de fauna de caza deportiva y culmina, hacia la década de 1970 con la inclusión de todos los reinos de la naturaleza así como su hábitat e interrelaciones. En nuestro país, y en general para Suramérica, es muy escasa la literatura especializada sobre vida silvestre, no existiendo facultades o carreras con dicha temática a pesar de la enorme biodiversidad de este continente. Algunas de las razones aducidas por el autor, que explican esta situación, son la influencia de la visión taxonómica de la naturaleza, privilegiada en Colombia por la Expedición Botánica, y la dificultad para incorporar la educación ambiental en el contexto académico universitario. Se concluye igualmente la escasa investigación en vida silvestre y el potencial que existe para desarrollarla en el campo de las ciencias naturales y sociales

  16. Avaliação de variedades híbridos interespecíficos e espécies silvestres de Manihot como fonte de resistência ao ácaro verde Mononychellus tanajoa (Bondar, 1938) (Acari , Tetranychidae)

    OpenAIRE

    Boaventura, Verônica de Jesus

    2013-01-01

    Este trabalho teve como objetivo, avaliar por meio de estudos biológicos em laboratório e avaliações em campo, a tolerância e/ou resistência de variedades, espécies silvestres e híbridos de Manihot ao ácaro verde M. tanajoa. Os aspectos biológicos do ácaro verde em espécies silvestres de Manihot foram avaliados no laboratório de Entomologia da Embrapa Mandioca e Fruticultura em nove acessos das seguintes espécies: M. esculenta ssp. flabellifolia, M. peruviana e M. carthaginensis ssp. Glaziovi...

  17. Horizontally transferred genes in the genome of Pacific white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Yuan, Jian-Bo; Zhang, Xiao-Jun; Liu, Cheng-Zhang; Wei, Jian-Kai; Li, Fu-Hua; Xiang, Jian-Hai

    2013-08-06

    In recent years, as the development of next-generation sequencing technology, a growing number of genes have been reported as being horizontally transferred from prokaryotes to eukaryotes, most of them involving arthropods. As a member of the phylum Arthropoda, the Pacific white shrimp Litopenaeus vannamei has to adapt to the complex water environments with various symbiotic or parasitic microorganisms, which provide a platform for horizontal gene transfer (HGT). In this study, we analyzed the genome-wide HGT events in L. vannamei. Through homology search and phylogenetic analysis, followed by experimental PCR confirmation, 14 genes with HGT event were identified: 12 of them were transferred from bacteria and two from fungi. Structure analysis of these genes showed that the introns of the two fungi-originated genes were substituted by shrimp DNA fragment, two genes transferred from bacteria had shrimp specific introns inserted in them. Furthermore, around other three bacteria-originated genes, there were three large DNA segments inserted into the shrimp genome. One segment was a transposon that fully transferred, and the other two segments contained only coding regions of bacteria. Functional prediction of these 14 genes showed that 6 of them might be related to energy metabolism, and 4 others related to defense of the organism. HGT events from bacteria or fungi were happened in the genome of L. vannamei, and these horizontally transferred genes can be transcribed in shrimp. This is the first time to report the existence of horizontally transferred genes in shrimp. Importantly, most of these genes are exposed to a negative selection pressure and appeared to be functional.

  18. Identification of cold responsive genes in Pacific white shrimp (Litopenaeus vannamei) by suppression subtractive hybridization.

    Science.gov (United States)

    Peng, Jinxia; Wei, Pinyuan; Chen, Xiuli; Zeng, Digang; Chen, Xiaohan

    2016-01-10

    The Pacific white shrimp (Litopenaeus vannamei) is one of the most widely cultured shrimp species in the world. Despite L. vannamei having tropical origins, it is being reared subtropically, with low temperature stress being one of the most severe threats to its growth, survival and distribution. To unravel the molecular basis of cold tolerance in L. vannamei, the suppression subtractive hybridization (SSH) platform was employed to identify cold responsive genes in the hepatopancreas of L. vannamei. Both forward and reverse cDNA libraries were constructed, followed by dot blot hybridization, cloning, sequence analysis and quantitative real-time PCR. These approaches identified 92 cold induced and 48 cold inhibited ESTs to give a total of 37 cold induced and 17 cold inhibited contigs. Some of the identified genes related to stress response or cell defense, such as tetraspanins (TSPANs), DEAD-box helicase, heat shock proteins (HSPs) and metallothionein (MT), which were more abundant in the forward SSH library than in the reverse SSH library. The most abundant Est was a tetraspanin-8 (TSPAN8) homolog dubbed LvTSPAN8. A multiple sequence alignment and transmembrane domain prediction was also performed for LvTSPAN8. LvTSPAN8 expression was also examined in the gills, muscle, heart and hepatopancreas following cold exposure and showed the highest expression levels in the hepatopancreas. Overall, this study was able to identify several known genes and novel genes via SSH that appear to be associated with cold stress and will help to provide further insights into the molecular mechanisms regulating cold tolerance in L. vannamei. Copyright © 2015 Elsevier B.V. All rights reserved.

  19. An integrin from shrimp Litopenaeus vannamei mediated microbial agglutination and cell proliferation.

    Science.gov (United States)

    Zhang, Ying; Wang, Leilei; Wang, Lingling; Wu, Ning; Zhou, Zhi; Song, Linsheng

    2012-01-01

    Integrins are a family of adhesion receptors which regulate cell proliferation, differentiation, leukocyte migration, and complement receptor-dependent phagocytosis. In invertebrates, as a cell adhesion receptor, β integrins play an important role for the balanced activation of immune defense responses especially during the encounter of infections. The present study attempts to characterize the immune functions of shrimp integrin (LvIntegrin) to have better understanding on the immune system and its regulation mechanisms in shrimps. A shrimp integrin was identified from the Pacific white shrimp Litopenaeus vannamei (designated as LvIntegrin). Its full-length cDNA was of 2621 bp with an open reading frame (ORF) of 2439 bp encoding a polypeptide of 812 amino acids. The mRNA expression of LvIntegrin was significantly up-regulated at 3, 6 and 12 h after Listonella anguillarum challenge. The cDNA fragment encoding β integrin domains (βA and hybrid domain) of LvIntegrin was recombined and expressed in Escherichia coli BL21(DE3)-pLysS. The recombinant protein (rLvIntegrin) could significantly agglutinate the tested microbe including E. coli JM109, L. anguillarum, Micrococcus luteus and Candida dattiladattila in the presence of divalent cations. Moreover, when NIH3T3 cells were cultured with rLvIntegrin, the proliferation rate increased significantly in a dose-dependent manner. LvIntegrin, a shrimp β integrin was identified from L. vannamei, shared several highly conserved features. LvIntegrin exhibited broad-spectrum agglutination activity towards both bacteria and fungi and could improve the proliferation of NIH3T3 cells, indicating that LvIntegrin is involved in the immune response against microbe challenge and regulation of cell proliferation as a cell adhesion receptor in shrimp.

  20. Analysis of Litopenaeus vannamei transcriptome using the next-generation DNA sequencing technique.

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    Chaozheng Li

    Full Text Available BACKGROUND: Pacific white shrimp (Litopenaeus vannamei, the major species of farmed shrimps in the world, has been attracting extensive studies, which require more and more genome background knowledge. The now available transcriptome data of L. vannamei are insufficient for research requirements, and have not been adequately assembled and annotated. METHODOLOGY/PRINCIPAL FINDINGS: This is the first study that used a next-generation high-throughput DNA sequencing technique, the Solexa/Illumina GA II method, to analyze the transcriptome from whole bodies of L. vannamei larvae. More than 2.4 Gb of raw data were generated, and 109,169 unigenes with a mean length of 396 bp were assembled using the SOAP denovo software. 73,505 unigenes (>200 bp with good quality sequences were selected and subjected to annotation analysis, among which 37.80% can be matched in NCBI Nr database, 37.3% matched in Swissprot, and 44.1% matched in TrEMBL. Using BLAST and BLAST2Go softwares, 11,153 unigenes were classified into 25 Clusters of Orthologous Groups of proteins (COG categories, 8171 unigenes were assigned into 51 Gene ontology (GO functional groups, and 18,154 unigenes were divided into 220 Kyoto Encyclopedia of Genes and Genomes (KEGG pathways. To primarily verify part of the results of assembly and annotations, 12 assembled unigenes that are homologous to many embryo development-related genes were chosen and subjected to RT-PCR for electrophoresis and Sanger sequencing analyses, and to real-time PCR for expression profile analyses during embryo development. CONCLUSIONS/SIGNIFICANCE: The L. vannamei transcriptome analyzed using the next-generation sequencing technique enriches the information of L. vannamei genes, which will facilitate our understanding of the genome background of crustaceans, and promote the studies on L. vannamei.

  1. Litopenaeus vannamei NF-κB is required for WSSV replication.

    Science.gov (United States)

    Qiu, Wei; Zhang, Shuang; Chen, Yong-Gui; Wang, Pei-Hui; Xu, Xiao-Peng; Li, Chao-zheng; Chen, Yi-Hong; Fan, Wen-Zhou; Yan, Hui; Weng, Shao-Ping; FrancisChan, Siuming; He, Jian-Guo

    2014-07-01

    Many viruses can hijack the host cell NF-κB as part of their life cycle, diverting NF-κB immune regulatory functions to favor their replications. There were several reports on the functions of Litopenaeus vannamei NF-κB (LvNF-κB) in White spot syndrome virus (WSSV) replication in vitro. Here, we studied the relationship between LvNF-κB family protein Dorsal (LvDorsal) and Relish (LvRelish) with WSSV replication in vivo. The expressions of LvDorsal and LvRelish were significantly upregulated by WSSV challenge. Virus loads and expression of viral envelope protein VP28 in LvDorsal or LvRelish silencing shrimps were significantly lower than the control shrimps injected with EGFP-dsRNA or PBS after challenge with 1×10(5) copies WSSV/shrimp. In addition to the LvDorsal activation of WSV069 (ie1) and WSV303 promoter that we have reported, LvRelish can also activate WSV069 (ie1) and WSV303 promoter by dual luciferase reporter assays through screening 40 WSSV gene promoters that have putative multiple NF-κB binding sites. The promoter activity of the WSV069 (ie1) by LvDorsal activation was significantly higher than that by LvRelish activation. WSSV replication in LvDorsal, LvRelish or WSV303 silencing shrimps were significantly inhibited. These results indicate that the L. vannamei NF-κB family proteins LvDorsal and LvRelish expressions are significantly activated by WSSV challenge and WSSV replication partially relied on the activations of LvDorsal and LvRelish in vivo. Copyright © 2014 Elsevier Ltd. All rights reserved.

  2. Bacterial community characterization of water and intestine of the shrimp Litopenaeus stylirostris in a biofloc system.

    Science.gov (United States)

    Cardona, Emilie; Gueguen, Yannick; Magré, Kevin; Lorgeoux, Bénédicte; Piquemal, David; Pierrat, Fabien; Noguier, Florian; Saulnier, Denis

    2016-07-19

    Biofloc technology (BFT), a rearing method with little or no water exchange, is gaining popularity in aquaculture. In the water column, such systems develop conglomerates of microbes, algae and protozoa, together with detritus and dead organic particles. The intensive microbial community presents in these systems can be used as a pond water quality treatment system, and the microbial protein can serve as a feed additive. The current problem with BFT is the difficulty of controlling its bacterial community composition for both optimal water quality and optimal shrimp health. The main objective of the present study was to investigate microbial diversity of samples obtained from different culture environments (Biofloc technology and clear seawater) as well as from the intestines of shrimp reared in both environments through high-throughput sequencing technology. Analyses of the bacterial community identified in water from BFT and "clear seawater" (CW) systems (control) containing the shrimp Litopenaeus stylirostris revealed large differences in the frequency distribution of operational taxonomic units (OTUs). Four out of the five most dominant bacterial communities were different in both culture methods. Bacteria found in great abundance in BFT have two principal characteristics: the need for an organic substrate or nitrogen sources to grow and the capacity to attach to surfaces and co-aggregate. A correlation was found between bacteria groups and physicochemical and biological parameters measured in rearing tanks. Moreover, rearing-water bacterial communities influenced the microbiota of shrimp. Indeed, the biofloc environment modified the shrimp intestine microbiota, as the low level (27 %) of similarity between intestinal bacterial communities from the two treatments. This study provides the first information describing the complex biofloc microbial community, which can help to understand the environment-microbiota-host relationship in this rearing system.

  3. Hemocyanin from Shrimp Litopenaeus vannamei Has Antiproliferative Effect against HeLa Cell In Vitro.

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    Liyuan Zheng

    Full Text Available Hemocyanin (HMC has been shown to participate in multiple roles of immune defence. In this study, we investigated the antiproliferative effect and underpinning mechanism of HMC from Litopenaeus vannamei in vitro. Sulforhodamine B (SRB assay indicated that HMC could dramatically inhibit the growth of HeLa cells, but not 293T cells under the same conditions. Moreover, typical morphological features of apoptosis in HeLa cells including the formation of apoptotic body-like vesicles, chromatin condensation and margination were observed by using 4, 6-diamidino-2- phenylindole dihydrochloride (DAPI staining and fluorescence analysis. An apoptotic DNA ladder from 180 to 300 bp was also detected. Furthermore, 10 variation proteins associated with apoptosis pathway, viz. G3PDH isoforms 1/2 (G3PDH1/2, aldosereductase, ectodemal dysplasia receptor associated death receptor domain isoform CRA_a (EDARADD, heat shock 60kD protein 1 variant 1 (HSP60, heat shock 70kDa protein 5 precursor (HSP70, heat shock protein 90kDa beta member 1 precursor (HSP90, 14-3-3 protein ζ/δ, Ran and ubiquitin activating enzyme E1(UBE1, were identified from HMC-treated HeLa cells by the proteomic and quantitative real-time RT-PCR strategies. Importantly, the reactive oxygen species (ROS, mitochondrial membrane potential (Δψm and caspase-9/3 activities were changed significantly in HMC-treated HeLa cells. Together, the data suggests that L. vannamei HMC mediates antiproliferative properties through the apoptosis mechanism involving the mitochondria triggered pathway.

  4. Selection for growth performance of tank-reared Pacific white shrimp, Litopenaeus vannamei

    Science.gov (United States)

    Andriantahina, Farafidy; Liu, Xiaolin; Huang, Hao; Xiang, Jianhai

    2013-05-01

    Seven growth-related traits were measured to assess the selection response and genetic parameters of the growth of Pacific white shrimp, Litopenaeus vannamei, which had been domesticated in tanks for more than four generations. Phenotypic and genetic parameters were evaluated and fitted to an animal model. Realized response was measured from the difference between the mean growth rates of selected and control families. Realized heritability was determined from the ratio of the selection responses and selection differentials. The animal model heritability estimate over generations was 0.44±0.09 for body weight (BW), and ranged from 0.21±0.08 to 0.37±0.06 for size traits. Genetic correlations of phenotypic traits were more variable (0.51-0.97), although correlations among various traits were high (>0.83). Across generations, BW and size traits increased, while selection response and heritability gradually decreased. Selection responses were 12.28%-23.35% for harvest weight and 3.58%-13.53% for size traits. Heritability estimates ranged from 0.34±0.09 to 0.48±0.15 for harvest weight and 0.17±0.01-0.38±0.11 for size traits. All phenotypic and genetic parameters differed between various treatments. To conclude, the results demonstrated a potential for mass selection of growth traits in L. vannamei. A breeding scheme could use this information to integrate the effectiveness constituent traits into an index to achieve genetic progress.

  5. Expansion of the Litopenaeus vannamei and Penaeus monodon peptidomes using transcriptome shotgun assembly sequence data.

    Science.gov (United States)

    Christie, Andrew E

    2014-09-15

    The shrimp Litopenaeus vannamei and Penaeus monodon are arguably the most important commercially farmed crustaceans. While expansion of their aquaculture has classically relied on improvements to rearing facilities, these options have largely been exhausted, and today a shift in focus is occurring, with increased investment in manipulating the shrimp themselves. Hormonal control is one strategy for increasing aquaculture output. However, to use it, one must first understand an animal's native hormonal systems. Here, transcriptome shotgun assembly (TSA) data were used to expand the peptidomes for L. vannamei and P. monodon. Via an established bioinformatics workflow, 41 L. vannamei and 25 P. monodon pre/preprohormone-encoding transcripts were identified, allowing for the prediction of 158 and 106 distinct peptide structures for these species, respectively. The identified peptides included isoforms of allatostatin A, B and C, as well as members the bursicon, CAPA, CCHamide, crustacean cardioactive peptide, crustacean hyperglycemic hormone, diuretic hormone 31, eclosion hormone, FLRFamide, GSEFLamide, intocin, leucokinin, molt-inhibiting hormone, myosuppressin, neuroparsin, neuropeptide F, orcokinin, orcomyotropin, pigment dispersing hormone, proctolin, red pigment concentrating hormone, RYamide, SIFamide, short neuropeptide F and tachykinin-related peptide families. While some of the predicted peptides are known L. vannamei and/or P. monodon isoforms (which vet the structures of many peptides identified previously via mass spectrometry and other means), most are described here for the first time. These data more than double the extant catalogs of L. vannamei and P. monodon peptides and provide platforms from which to launch future physiological studies of peptidergic signaling in these two commercially important species. Copyright © 2014 Elsevier Inc. All rights reserved.

  6. SNP Discovery in the Transcriptome of White Pacific Shrimp Litopenaeus vannamei by Next Generation Sequencing

    Science.gov (United States)

    Yu, Yang; Wei, Jiankai; Zhang, Xiaojun; Liu, Jingwen; Liu, Chengzhang; Li, Fuhua; Xiang, Jianhai

    2014-01-01

    The application of next generation sequencing technology has greatly facilitated high throughput single nucleotide polymorphism (SNP) discovery and genotyping in genetic research. In the present study, SNPs were discovered based on two transcriptomes of Litopenaeus vannamei (L. vannamei) generated from Illumina sequencing platform HiSeq 2000. One transcriptome of L. vannamei was obtained through sequencing on the RNA from larvae at mysis stage and its reference sequence was de novo assembled. The data from another transcriptome were downloaded from NCBI and the reads of the two transcriptomes were mapped separately to the assembled reference by BWA. SNP calling was performed using SAMtools. A total of 58,717 and 36,277 SNPs with high quality were predicted from the two transcriptomes, respectively. SNP calling was also performed using the reads of two transcriptomes together, and a total of 96,040 SNPs with high quality were predicted. Among these 96,040 SNPs, 5,242 and 29,129 were predicted as non-synonymous and synonymous SNPs respectively. Characterization analysis of the predicted SNPs in L. vannamei showed that the estimated SNP frequency was 0.21% (one SNP per 476 bp) and the estimated ratio for transition to transversion was 2.0. Fifty SNPs were randomly selected for validation by Sanger sequencing after PCR amplification and 76% of SNPs were confirmed, which indicated that the SNPs predicted in this study were reliable. These SNPs will be very useful for genetic study in L. vannamei, especially for the high density linkage map construction and genome-wide association studies. PMID:24498047

  7. Identification and functional characterization of an endoplasmic reticulum oxidoreductin 1-α gene in Litopenaeus vannamei.

    Science.gov (United States)

    Zhang, Ze-Zhi; Yuan, Kai; Yue, Hai-Tao; Yuan, Feng-Hua; Bi, Hai-Tao; Weng, Shao-Ping; He, Jian-Guo; Chen, Yi-Hong

    2016-04-01

    In the current study, full-length sequence of endoplasmic reticulum oxidoreductin 1-α (LvERO1-α) was cloned from Litopenaeus vannamei. Real-time RT-PCR results showed that LvERO1-α was highly expressed in hemocytes, gills, and intestines. White spot syndrome virus (WSSV) challenge was performed, and the expression of LvERO1-α and two other downstream genes of the double-stranded RNA-activated protein kinase-like ER kinase-eIF2α (PERK-α) pathway, namely, homocysteine-induced endoplasmic reticulum protein (LvHERP) and acylamino-acid-releasing enzyme (LvAARE), strongly increased in the hemocytes. Flow cytometry assay results indicated that the apoptosis rate of L. vannamei hemocytes in the LvERO1-α knockdown group was significantly lower than that of the controls. Moreover, shrimps with knockdown expression of LvERO1-α exhibited decreased cumulative mortality upon WSSV infection. Downregulation of L. vannamei immunoglobulin-binding protein (LvBip), which had been proven to induce unfolded protein response (UPR) in L. vannamei, did not only upregulate LvERO1-α, LvHERP, and LvAARE in hemocytes, but also increased their apoptosis rate, as well as the shrimp cumulative mortality. Furthermore, reporter gene assay results showed that the promoter of LvERO1-α was activated by L. vannamei activating transcription factor 4, thereby confirming that LvERO1-α was regulated by the PERK-eIF2α pathway. These results suggested that LvERO1-α plays a critical role in WSSV-induced apoptosis, which likely occurs through the WSSV-activated PERK-eIF2α pathway. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. Impact of ammonia exposure on coagulation in white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Chang, Zhong-Wen; Chiang, Pei-Chi; Cheng, Winton; Chang, Chin-Chyuan

    2015-08-01

    Ammonia (un-ionized plus ionized ammonia as nitrogen), the end product of protein catabolism, is produced by decomposing organic matter. In aquaculture, shrimp are commonly exposed to high concentrations of ammonia that induces immunological and histological changes. The purpose of this study was to evaluate the effects on hemolymph coagulation time, transglutaminase (TG) activity as well as TG and clottable protein (CP) genes expressions in Litopenaeus vannamei when exposed to ambient ammonia-nitrogen (N) at 0, 1, 5, and 10mg/L for 0, 2, and 7 days. The actual concentrations in control and tests solution were 0.001, 1.15, 5.11, and 11.68mg/L for ammonia-N, and 7×10(-5), 0.080, 0.357, and 0.815mg/L for NH3-N (unionized ammonia). Delayed coagulation time following exposure to 5 and 10mg/L of ambient ammonia-N for 7 days, and increased transglutaminase (TG) activity following exposure to 5 and 1mg/L of ambient ammonia-N for 2 and 7 days, respectively, were observed. Downregulated TG expression and upregulated clottable protein (CP) expression in the hemocytes of L. vannamei exposed to 10 and 5mg/L of ambient ammonia-N for 2 and 7 days, respectively, were shown. These results indicated that ambient ammonia-N (>5mg/L) and NH3-N (>0.357mg/L) interrupted coagulation and down-regulated TG gene expression in L. vannamei, which caused ecotoxicity on immune deficiencies and may contribute the increased susceptibility to infection by pathogens. Copyright © 2015 Elsevier Inc. All rights reserved.

  9. Identification and Function of Myeloid Differentiation Factor 88 (MyD88) in Litopenaeus vannamei

    Science.gov (United States)

    Zhang, Shuang; Li, Chao-Zheng; Yan, Hui; Qiu, Wei; Chen, Yong-Gui; Wang, Pei-Hui; Weng, Shao-Ping; He, Jian-Guo

    2012-01-01

    Myeloid differentiation factor 88 (MyD88) is a universal and essential signaling protein in Toll-like receptor/interleukin-1 receptor-induced activation of nuclear factor-kappa B. In this study, two MyD88 protein variants (LvMyD88 and LvMyD88-1) were identified in Litopenaeus vannamei. The LvMyD88 cDNA is 1,848 bp in length and contains an open reading frame (ORF) of 1,428 bp, whereas the LvMyD88-1 cDNA is 1,719 bp in length and has an ORF of 1,299 bp. Both variants encode proteins with death and Toll/interleukin-1 receptor domains and share 91% sequence identity. In healthy L. vannamei, the LvMyD88 genes were highly expressed in hemocytes but at a low level in the hepatopancreas. The LvMyD88s expression was induced in hemocytes after challenge with lipopolysaccharide, CpG-ODN2006, Vibrio parahaemolyticus, Staphyloccocus aureus, and white spot syndrome virus, but not by poly I∶C. Overexpression of LvMyD88 and LvMyD88-1 in Drosophila Schneider 2 cells led to activation of antimicrobial peptide genes and wsv069 (ie1), wsv303, and wsv371. These results suggested that LvMyD88 may play a role in antibacterial and antiviral response in L. vannamei. To our knowledge, this is the first report on MyD88 in shrimp and a variant of MyD88 gene in invertebrates. PMID:23071706

  10. Horizontally transferred genes in the genome of Pacific white shrimp, Litopenaeus vannamei

    Science.gov (United States)

    2013-01-01

    Background In recent years, as the development of next-generation sequencing technology, a growing number of genes have been reported as being horizontally transferred from prokaryotes to eukaryotes, most of them involving arthropods. As a member of the phylum Arthropoda, the Pacific white shrimp Litopenaeus vannamei has to adapt to the complex water environments with various symbiotic or parasitic microorganisms, which provide a platform for horizontal gene transfer (HGT). Results In this study, we analyzed the genome-wide HGT events in L. vannamei. Through homology search and phylogenetic analysis, followed by experimental PCR confirmation, 14 genes with HGT event were identified: 12 of them were transferred from bacteria and two from fungi. Structure analysis of these genes showed that the introns of the two fungi-originated genes were substituted by shrimp DNA fragment, two genes transferred from bacteria had shrimp specific introns inserted in them. Furthermore, around other three bacteria-originated genes, there were three large DNA segments inserted into the shrimp genome. One segment was a transposon that fully transferred, and the other two segments contained only coding regions of bacteria. Functional prediction of these 14 genes showed that 6 of them might be related to energy metabolism, and 4 others related to defense of the organism. Conclusions HGT events from bacteria or fungi were happened in the genome of L. vannamei, and these horizontally transferred genes can be transcribed in shrimp. This is the first time to report the existence of horizontally transferred genes in shrimp. Importantly, most of these genes are exposed to a negative selection pressure and appeared to be functional. PMID:23914989

  11. LvDJ-1 plays an important role in resistance against Vibrio alginolyticus in Litopenaeus vannamei.

    Science.gov (United States)

    Huang, Mingzhu; Liu, Yuan; Xie, Chenying; Wang, Wei-Na

    2015-05-01

    DJ-1 was first identified as an oncogene that transformed mouse NIH3T3 cells in cooperation with activated Ras. It has since exhibited a variety of functions in a range of organisms. In this study, the DJ-1 gene in Litopenaeus vannamei (LvDJ-1) was identified and characterized. A recombinant protein LvDJ-1 was produced in Pichia pastoris. LvDJ-1 expression in vivo was knocked down by dsRNA-mediated RNA interference (RNAi), which led to significantly decreased levels of LvDJ-1 mRNA and protein. When the L. vannamei were challenged with RNAi and Vibrio alginolyticus, the transcription and expression of copper zinc superoxide dismutase (LvCZSOD) in the hepatopancreas were dramatically lower in shrimp with knocked down LvDJ-1 than in controls. Transcription and expression of P53 (LvP53) were significantly higher in shrimp lacking LvDJ-1 than in controls. Hepatopancreas samples were analyzed using real time polymerase chain reaction and Western blot. Moreover, blood samples from the shrimp, assessed with flow cytometry, showed significant increases in respiratory burst and apoptosis in those lacking LvDJ-1 compared to the controls. Cumulative mortality in the shrimp lacking LvDJ-1 was significantly different from that in the control group after challenge with V. alginolyticus. Altogether, the results prove that LvDJ-1 regulates apoptosis and antioxidant activity, and that these functions play an important role in L. vannamei resistance against V. alginolyticus. Copyright © 2015 Elsevier Ltd. All rights reserved.

  12. Transcriptome Analysis of the Hepatopancreas in the Pacific White Shrimp (Litopenaeus vannamei) under Acute Ammonia Stress

    Science.gov (United States)

    Lu, Xia; Kong, Jie; Luan, Sheng; Dai, Ping; Meng, Xianhong; Cao, Baoxiang; Luo, Kun

    2016-01-01

    In the practical farming of Litopenaeus vannamei, the intensive culture system and environmental pollution usually results in a high concentration of ammonia, which usually brings large detrimental effects to shrimp, such as increasing the susceptibility to pathogens, reducing growth, decreasing osmoregulatory capacity, increasing the molting frequency, and even causing high mortality. However, little information is available on the molecular mechanisms of the detrimental effects of ammonia stress in shrimp. In this study, we performed comparative transcriptome analysis between ammonia-challenged and control groups from the same family of L. vannamei to identify the key genes and pathways response to ammonia stress. The comparative transcriptome analysis identified 136 significantly differentially expressed genes that have high homologies with the known proteins in aquatic species, among which 94 genes are reported potentially related to immune function, and the rest of the genes are involved in apoptosis, growth, molting, and osmoregulation. Fourteen GO terms and 6 KEGG pathways were identified to be significantly changed by ammonia stress. In these GO terms, 13 genes have been studied in aquatic species, and 11 of them were reported potentially involved in immune defense and two genes were related to molting. In the significantly changed KEGG pathways, all the 7 significantly changed genes have been reported in shrimp, and four of them were potentially involved in immune defense and the other three were related to molting, defending toxicity, and osmoregulation, respectively. In addition, majority of the significantly changed genes involved in nitrogen metabolisms that play an important role in reducing ammonia toxicity failed to perform the protection function. The present results have supplied molecular level support for the previous founding of the detrimental effects of ammonia stress in shrimp, which is a prerequisite for better understanding the molecular

  13. Transcriptomic analysis of the hepatopancreas induced by eyestalk ablation in shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Lee, Ji-Hyun; Suryaningtyas, Indyaswan T; Yoon, Tae-Ho; Shim, Jeong Min; Park, Hyun; Kim, Hyun-Woo

    2017-12-01

    Although eyestalk ablation (ESA) is currently considered the most effective method to facilitate molting and maturation, its physiological responses are still not clearly explained in decapod crustaceans. In this study, we analyzed the hepatopancreatic transcriptomes of Litopenaeus vannamei after ESA using the Illumina Miseq platform. After screening 53,029 contigs with high cutoff values (fold change>|10|; P-value1), we were able to identify 105 differentially expressed genes (DEGs), of which 100 were up-regulated and five were down-regulated. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that many DEGs were involved in the synthetic pathways for glycerol and trehalose, which are known to function as the major protectants under conditions of low temperature and osmotic stress in arthropods. Additional analysis of the other DEGs enabled us to classify them in four categories: immunity; cellular trafficking; transcriptional regulation; molting and maturation. Many DEGs were involved in immunity and stress responses, in particular the proPO activation system, which is the major immune and wound-healing system in arthropods. In addition to immunity and stress responses, we were also able to identify DEGs involved in molting and maturation processes (e.g., group I chitinase), as well as those involved in hormone metabolism and trafficking. Collectively, based on the transcriptomic analysis, ESA causes not only stress and immune responses, but also molting and maturation in L. vannamei. The DEGs identified in this study could be useful markers to understand the physiological responses that ESA induces in shrimp, such as molting, maturation, and immunity. Copyright © 2017 Elsevier Inc. All rights reserved.

  14. Whole Transcriptome Analysis Provides Insights into Molecular Mechanisms for Molting in Litopenaeus vannamei

    Science.gov (United States)

    Gao, Yi; Zhang, Xiaojun; Wei, Jiankai; Sun, Xiaoqing; Yuan, Jianbo; Li, Fuhua; Xiang, Jianhai

    2015-01-01

    Molting is one of the most important biological processes in shrimp growth and development. All shrimp undergo cyclic molting periodically to shed and replace their exoskeletons. This process is essential for growth, metamorphosis, and reproduction in shrimp. However, the molecular mechanisms underlying shrimp molting remain poorly understood. In this study, we investigated global expression changes in the transcriptomes of the Pacific white shrimp, Litopenaeus vannamei, the most commonly cultured shrimp species worldwide. The transcriptome of whole L. vannamei was investigated by RNA-sequencing (RNA-seq) throughout the molting cycle, including the inter-molt (C), pre-molt (D0, D1, D2, D3, D4), and post-molt (P1 and P2) stages, and 93,756 unigenes were identified. Among these genes, we identified 5,117 genes differentially expressed (log2ratio ≥1 and FDR ≤0.001) in adjacent molt stages. The results were compared against the National Center for Biotechnology Information (NCBI) non-redundant protein/nucleotide sequence database, Swiss-Prot, PFAM database, the Gene Ontology database, and the Kyoto Encyclopedia of Genes and Genomes database in order to annotate gene descriptions, associate them with gene ontology terms, and assign them to pathways. The expression patterns for genes involved in several molecular events critical for molting, such as hormone regulation, triggering events, implementation phases, skelemin, immune responses were characterized and considered as mechanisms underlying molting in L. vannamei. Comparisons with transcriptomic analyses in other arthropods were also performed. The characterization of major transcriptional changes in genes involved in the molting cycle provides candidates for future investigation of the molecular mechanisms. The data generated in this study will serve as an important transcriptomic resource for the shrimp research community to facilitate gene and genome annotation and to characterize key molecular processes

  15. Analysis of Litopenaeus vannamei Transcriptome Using the Next-Generation DNA Sequencing Technique

    Science.gov (United States)

    Li, Chaozheng; Weng, Shaoping; Chen, Yonggui; Yu, Xiaoqiang; Lü, Ling; Zhang, Haiqing; He, Jianguo; Xu, Xiaopeng

    2012-01-01

    Background Pacific white shrimp (Litopenaeus vannamei), the major species of farmed shrimps in the world, has been attracting extensive studies, which require more and more genome background knowledge. The now available transcriptome data of L. vannamei are insufficient for research requirements, and have not been adequately assembled and annotated. Methodology/Principal Findings This is the first study that used a next-generation high-throughput DNA sequencing technique, the Solexa/Illumina GA II method, to analyze the transcriptome from whole bodies of L. vannamei larvae. More than 2.4 Gb of raw data were generated, and 109,169 unigenes with a mean length of 396 bp were assembled using the SOAP denovo software. 73,505 unigenes (>200 bp) with good quality sequences were selected and subjected to annotation analysis, among which 37.80% can be matched in NCBI Nr database, 37.3% matched in Swissprot, and 44.1% matched in TrEMBL. Using BLAST and BLAST2Go softwares, 11,153 unigenes were classified into 25 Clusters of Orthologous Groups of proteins (COG) categories, 8171 unigenes were assigned into 51 Gene ontology (GO) functional groups, and 18,154 unigenes were divided into 220 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. To primarily verify part of the results of assembly and annotations, 12 assembled unigenes that are homologous to many embryo development-related genes were chosen and subjected to RT-PCR for electrophoresis and Sanger sequencing analyses, and to real-time PCR for expression profile analyses during embryo development. Conclusions/Significance The L. vannamei transcriptome analyzed using the next-generation sequencing technique enriches the information of L. vannamei genes, which will facilitate our understanding of the genome background of crustaceans, and promote the studies on L. vannamei. PMID:23071809

  16. Identification and functional characterization of the TAB2 gene from Litopenaeus vannamei.

    Science.gov (United States)

    Wang, Sheng; Li, Haoyang; Qian, Zhe; Song, Xuan; Zhang, Zijian; Zuo, Hongliang; Xu, Xiaopeng; Weng, Shaoping; He, Jianguo; Li, Chaozheng

    2015-10-01

    In Drosophila, TAB2, an important intermediate in the IMD signaling pathway, plays critical roles in the innate immune response in response to bacterial and viral infection. However, the role of TAB-related proteins in the immune response of shrimp has not yet been established. Here, we reported the identification of a TAB2-like gene in Litopenaeus vannamei designated as LvTAB2. The full-length cDNA of LvTAB2 was 2160 bp with an open reading frame of 1827 bp, which encoded a putative protein of 608 amino acids including a ubiquitin binding domain (CUE) at the N-terminal and a Zinc Finger domain (ZnF) at the C-terminus. Real-time RT-PCR analysis showed that LvTAB2 was expressed in all tested tissues and the expression levels of LvTAB2 in gills and hemocytes were positively induced in response to LPS, Vibrio parahemolyticus and White Spot Syndrome Virus (WSSV) challenges. Dual luciferase reporter assays demonstrated that LvTAB2 was able to induce the expression of antimicrobial peptide (AMP) genes, including Drosophila Attacin A and shrimp Penaeidins. Interestingly, over-expression of LvTAB2 could up-regulate the promoter activities of L. vannamei Vago1, Vago3 and Vago4 genes in S2 cells. To our knowledge, it was the first report that TAB2 participated in innate immune signaling to regulate the expression of Vago genes in invertebrates. Moreover, RNAi-mediated knockdown of LvTAB2 enhanced sensitivity of L. vannamei to Vibrio parahaemolyticus infection and caused elevated virus loads after WSSV infection. We suggested that the LvTAB2 may play important roles in the shrimp innate immunity. Copyright © 2015 Elsevier Ltd. All rights reserved.

  17. Energy metabolism and metabolomics response of Pacific white shrimp Litopenaeus vannamei to sulfide toxicity.

    Science.gov (United States)

    Li, Tongyu; Li, Erchao; Suo, Yantong; Xu, Zhixin; Jia, Yongyi; Qin, Jian G; Chen, Liqiao; Gu, Zhimin

    2017-02-01

    The toxicity and poisoning mechanisms of sulfide were studied in Litopenaeus vannamei from the perspective of energy metabolism and metabolomics. The lethal concentrations of sulfide in L. vannamei (LC50) at 24h, 48h, 72h, and 96h were determined. Sulfide at a concentration of 0, 1/10 (425.5μg/L), and 1/5 (851μg/L) of the LC50 at 96h was used to test the metabolic responses of L. vannamei for 21days. The chronic exposure of shrimp to a higher sulfide concentration of 851μg/L decreased shrimp survival but did not affect weight gain or the hepatopancreas index. The glycogen content in the hepatopancreas and muscle and the activity of hepatopancreas cytochrome C oxidase of the shrimp exposed to all sulfide concentrations were significantly lower, and the serum glucose and lactic acid levels and lactic acid dehydrogenase activity were significantly lower than those in the control. Metabolomics assays showed that shrimp exposed to sulfide had lower amounts of serum pyruvic acid, succinic acid, glycine, alanine, and proline in the 425.5μg/L group and phosphate, succinic acid, beta-alanine, serine, and l-histidine in the 851μg/L group than in the control. Chronic sulfide exposure could disturb protein synthesis in shrimp but enhance gluconeogenesis and substrate absorption for ATP synthesis and tricarboxylic acid cycles to provide extra energy to cope with sulfide stress. Chronic sulfide exposure could adversely affect the health status of L. vannamei, as indicated by the high amounts of serum n-ethylmaleamic acid, pyroglutamic acid, aspartic acid and phenylalanine relative to the control. This study indicates that chronic exposure of shrimp to sulfide can decrease health and lower survival through functional changes in gluconeogenesis, protein synthesis and energy metabolism. Copyright © 2016 Elsevier B.V. All rights reserved.

  18. Identification and functional characterization of heat shock transcription factor1 in Litopenaeus vannamei.

    Science.gov (United States)

    Yan, Hui; Zhang, Shuang; Li, Xiao-Yun; Yuan, Feng-Hua; Qiu, Wei; Chen, Yong-Gui; Weng, Shao-Ping; He, Jian-Guo; Chen, Yi-Hong

    2014-03-01

    Heat shock transcription factors belong to the heat shock factor (HSF) protein family, which are involved in heat shock protein (HSP) gene regulation. They are critical for cell survival upon exposure to harmful conditions. In this study, we identified and characterized a HSF1 (LvHSF1) gene in Litopenaeus vannamei, with a full-length cDNA of 2841 bp and an open reading frame encoding a putative protein of 632 amino acids. Through multiple sequence alignment and phylogenetic analysis, it was revealed that LvHSF1 was closed to insect HSF family, which contained a highly conserved DNA-binding domain, oligomerization domains with HR-A/B, and a nuclear localization signal. Tissues distribution showed that LvHSF1 was widely expressed in all tissues tested. And it was upregulated in hemocytes and gills after Vibrio alginolyticus or Staphylococcus aureus infection. Dual-luciferase reporter assays indicated that LvHSF1 activated the promoters of L. vannamei HSP70 (LvHSP70) and L. vannamei Cactus (LvCactus), while inhibited the expressions of Drosophila antimicrobial peptide (AMP) Atta, Mtk, and L. vannamei AMP PEN4 through NF-κB signal transduction pathway modification. Knocked-down expression of LvHSF1 by dsRNA resulted in downregulations of LvHSP70 and LvCactus, as well as cumulative mortality decreasing under V. alginolyticus or S. aureus infection in L. vannamei. Taken together, our data strongly suggest that LvHSF1 is involved in LvHSP70 regulation, therefore plays a great role in stress resistance. And it also takes part in LvCactus/LvDorsal feedback regulatory pathway modification of L. vannamei, which is in favor of V. alginolyticus or S. aureus infection. Copyright © 2014 Elsevier Ltd. All rights reserved.

  19. Cloning and characterization of a novel hemocyanin variant LvHMCV4 from shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Lu, Xin; Lu, Hui; Guo, Lingling; Zhang, Zehui; Zhao, Xianliang; Zhong, Mingqi; Li, Shengkang; Zhang, Yueling

    2015-10-01

    Recently, we found 3 variants of hemocyanin subunit with higher molecular weight in shrimp Litopenaeus vannamei (Named as LvHMCV1-3). In this study, a novel L. vannamei hemocyanin variant (Named as LvHMCV4) was further cloned and characterized. Bioinformatic analysis predicted that LvHMCV4 contains one open reading frame of 2137 bp and encodes a polypeptide of 678 amino acids. It shares 84-99% cDNA sequences identity to that of the classical form of L. vannamei hemocyanin (LvHMC, AJ250830.1) and LvHMCV1-3. LvHMCV4 possesses a conserved structure characteristic of the hemocyanin family and can be clustered into one branch along with other arthropod hemocyanins in a phylogenetic tree. Further, the full-length DNA of LvHMCV4 contains 2660 bp and two introns, which are located at the 80-538 bp and 2063-2227 bp regions, respectively. In addition, the mRNA transcript of LvHMCV4 was expressed highly in the hepatopancreas, lymphoid, brain and hemocytes, and weakly in the heart, intestine and gill, while no expression was found in the muscle, stomach and gut. Infection by Escherichia coli K12, Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio fluvialis, Streptococcus pyogenes or Staphylococcus aureus up-regulated significantly LvHMCV4 mRNA expression in the hepatopancreas. Furthermore, the recombinant protein of LvHMCV4 (rLvHMCV4) was prepared, which showed agglutination activities against six pathogenic bacteria at concentrations ranging from 15.6 to 125 μg/ml. When co-injected with V. parahaemolyticus in L.vannamei, rLvHMCV4 significantly increased the survival rate after 48 h injection. Together, these studies suggested that hemocyanin variant, LvHMCV4, might be involved in shrimp resistance to pathogenic infection. Copyright © 2015 Elsevier Ltd. All rights reserved.

  20. Identification and characterization of a mitochondrial unfolded protein response transcription factor ATFS-1 in Litopenaeus vannamei.

    Science.gov (United States)

    Chen, Yong-Gui; Yue, Hai-Tao; Zhang, Ze-Zhi; Yuan, Feng-Hua; Bi, Hai-Tao; Yuan, Kai; Weng, Shao-Ping; He, Jian-Guo; Chen, Yi-Hong

    2016-07-01

    A mitochondrial specific stress response termed mitochondrial unfolded protein response (UPR(mt)) is activated in responding to disturbance of protein homeostasis in mitochondria. The activating transcription factor associated with stress-1 (designated as ATFS-1) is the key regulator of UPR(mt). To investigating the roles of ATFS-1 (LvATFS-1) in Litopenaeus vannamei mitochondrial stress remission and immunity, it's full length cDNA was cloned. The open reading frame of LvATFS-1 was 1, 557 bp in length, deducing to a 268 amino acids protein. LvATFS-1 was highly expressed in muscle, hemocytes and eyestalk. Subcellular location assays showed that N-terminal of LvATFS-1 contained a mitochondrial targeting sequence, which could directed the fused EGFP located to mitochondria. And the C-terminal of LvATFS-1, which had a nuclear localization signal, expressed in nucleus. The in vitro experiments verified that LvATFS-1 could reduced the level of intracellular reactive oxygen species (ROS). And results of real-time RT-PCR indicated that LvATFS-1 might scavenge excess ROS via ROS-eliminating genes regulation. Reporter gene assays showed that LvATFS-1 could upregulated the expression of the antimicrobial peptide genes in Drosophila Schneider 2 cells. Results of real-time RT-PCR showed that Vibrio alginolyticus or white spot syndrome virus (WSSV) infection induced the expression of LvATFS-1. And knocked-down LvATFS-1 by RNAi resulted in a higher cumulative mortality of L. vannamei upon V. alginolyticus or WSSV infection. These results suggested that LvATFS-1 not only rolled in mitochondrial specific stress responding, but also important for L. vannamei immunologic defence. Copyright © 2015 Elsevier Ltd. All rights reserved.

  1. Transcriptome Analysis of the Hepatopancreas in the Pacific White Shrimp (Litopenaeus vannamei under Acute Ammonia Stress.

    Directory of Open Access Journals (Sweden)

    Xia Lu

    Full Text Available In the practical farming of Litopenaeus vannamei, the intensive culture system and environmental pollution usually results in a high concentration of ammonia, which usually brings large detrimental effects to shrimp, such as increasing the susceptibility to pathogens, reducing growth, decreasing osmoregulatory capacity, increasing the molting frequency, and even causing high mortality. However, little information is available on the molecular mechanisms of the detrimental effects of ammonia stress in shrimp. In this study, we performed comparative transcriptome analysis between ammonia-challenged and control groups from the same family of L. vannamei to identify the key genes and pathways response to ammonia stress. The comparative transcriptome analysis identified 136 significantly differentially expressed genes that have high homologies with the known proteins in aquatic species, among which 94 genes are reported potentially related to immune function, and the rest of the genes are involved in apoptosis, growth, molting, and osmoregulation. Fourteen GO terms and 6 KEGG pathways were identified to be significantly changed by ammonia stress. In these GO terms, 13 genes have been studied in aquatic species, and 11 of them were reported potentially involved in immune defense and two genes were related to molting. In the significantly changed KEGG pathways, all the 7 significantly changed genes have been reported in shrimp, and four of them were potentially involved in immune defense and the other three were related to molting, defending toxicity, and osmoregulation, respectively. In addition, majority of the significantly changed genes involved in nitrogen metabolisms that play an important role in reducing ammonia toxicity failed to perform the protection function. The present results have supplied molecular level support for the previous founding of the detrimental effects of ammonia stress in shrimp, which is a prerequisite for better

  2. Biochemical changes of Litopenaeus vannamei and Fenneropenaeus indicus in the different stages of WSSV infection

    Directory of Open Access Journals (Sweden)

    Ramachandran Shalini

    2013-01-01

    Full Text Available Objective: To find out the difference in the proximate composition and fatty acid profile of both the species of shrimp Litopenaeus vannamei (L. vannamei and Fenneropenaeus indicus (F. indicus infected with different stages of white spot syndrome virus (WSSV. Methods: Standard methods were followed by estimating the proximate composition and fatty acid analysis. Each fish specimens were beheaded, eviscerated and filleted manually. The tissue samples were oven dried at 67 °C for 24 h. Then the samples were grounded finely with pestle and mortar. The saponified samples were cooled at room temperature for 25 min. They were acidified and methylated by adding 2 mL 54% 6 mol/L HCL in 46% aqueous methanol and incubated at 80 °C for 10 min in water bath. Following the base wash step, the fatty acid methyl esters were cleaned in anhydrous sodium sulphate and then transferred into gas chromatograph sample vial for analysis. Fatty acid methyl esters were separated by gas chromatograph. Results: The proximate composition was higher in the both control tissue than the three (low, moderate, severe infected ones. For L. vannamei and F. indicus, the carbohydrates are 5.07% and 6.18%, and the proteins are 25.01% and 22.17%, respectively. Lipid level recorded was little higher in the shrimps maintained and showed severe sign of WSSV infection than the control and the fatty acid profile result revealed that saturated fatty acids and monounsaturated fatty acid was in higher [48.72% (Severe & 16.87% (low] L. vannamei. In the polyunsaturated fatty acid, F. indicus was 40.47% (low. Conclusions: Our study showed that the healthy shrimps are nutritionally rich than the WSSV affected shrimps.

  3. Biochemical changes of Litopenaeus vannamei and Fenneropenaeus indicus in the different stages of WSSV infection

    Directory of Open Access Journals (Sweden)

    Ramachandran Shalini

    2013-08-01

    Full Text Available Objective: To find out the difference in the proximate composition and fatty acid profile of both the species of shrimp Litopenaeus vannamei (L. vannamei and Fenneropenaeus indicus (F. indicus infected with different stages of white spot syndrome virus (WSSV. Methods: Standard methods were followed by estimating the proximate composition and fatty acid analysis. Each fish specimens were beheaded, eviscerated and filleted manually. The tissue samples were oven dried at 67 °C for 24 h. Then the samples were grounded finely with pestle and mortar. The saponified samples were cooled at room temperature for 25 min. They were acidified and methylated by adding 2 mL 54% 6 mol/L HCL in 46% aqueous methanol and incubated at 80 °C for 10 min in water bath. Following the base wash step, the fatty acid methyl esters were cleaned in anhydrous sodium sulphate and then transferred into gas chromatograph sample vial for analysis. Fatty acid methyl esters were separated by gas chromatograph. Results: The proximate composition was higher in the both control tissue than the three (low, moderate, severe infected ones. For L. vannamei and F. indicus, the carbohydrates are 5.07% and 6.18%, and the proteins are 25.01% and 22.17%, respectively. Lipid level recorded was little higher in the shrimps maintained and showed severe sign of WSSV infection than the control and the fatty acid profile result revealed that saturated fatty acids and monounsaturated fatty acid was in higher [48.72% (Severe & 16.87% (low] L. vannamei. In the polyunsaturated fatty acid, F. indicus was 40.47% (low. Conclusions: Our study showed that the healthy shrimps are nutritionally rich than the WSSV affected shrimps.

  4. PARÁMETROS HEMATOLÓGICOS EN POLLUELOS DE PSITÁCIDOS EN CAUTIVERIO DE ORIGEN SILVESTRE

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    J. A. Herrera

    2013-01-01

    Full Text Available El objetivo de este estudio fue describir los parámetros hematológicos en 69 polluelos en cautiverio de origen silvestre, clínicamente sanos, menores de tres meses de edad, en cuatro especies de psitácidos: Pionus senilis (n = 18, Amazona albifrons (n = 15, Amazona autumnalis (n = 25 y Amazona oratrix (n = 11, procedentes de la región central de México. Para determinar dichos parámetros, de cada ejemplar se obtuvieron 500 μl de sangre por punción de la vena braquial. En las cuatro especies citadas, el porcentaje del hematocrito fue de 56,4 ± 3,36; 51 ± 6,52; 47,6 ± 6,47 y 53,8 ± 9,26; el VCM en fl fue de 184,6 ± 13,4; 142,0 ± 25,4; 106,3 ± 11,7 y 162,3 ± 71,9; la cuenta eritrocítica fue de 4,7 ± 2,1 x10 6 /μl; 6,1 ± 8,2 x10 6 /μl; 6,9 ± 1,2 x10 6 /μl y 5,4 ± 1,1 x10 6 /μl; y la cuenta leucocitaria de 3,06 ± 0,15 x10 3 /μl; 3,62 ± 0,24 x10 3 /μl; 4,48 ± 0,36 x10 3 /μl y 3,56 ± 0,71 x10 3 /μl respectivamente para P. senilis , A. albifrons , A. autumnalis y A. oratrix . Se constató tendencia a la heterofilia en A. albifrons y A. oratrix , lo cual puede ser de gran utilidad para un diagnóstico cada vez más preciso en la clínica de aves en cautiverio.

  5. Fenologia de espécies silvestres de macieira como polinizadora das cultivares gala e fuji Phenology of wild apple species like pollinators of gala and fuji cultivars

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    José Luiz Petri

    2008-12-01

    Full Text Available A escolha correta de cultivares polinizadoras na macieira é determinante para a obtenção de altos rendimentos. Muitas regiões produtoras de maçãs do mundo utilizam macieiras silvestres com o fim específico de polinização, porém existem poucas informações quanto ao uso dessas espécies nas condições climáticas do Sul do Brasil. O objetivo deste trabalho foi avaliar o comportamento fenológico de espécies silvestres de macieira quanto à floração em comparação as cultivares comerciais 'Gala' e 'Fuji', nas condições climáticas do Sul do Brasil, em Caçador-SC (latitude 26º42'32" sul, longitude 51º00'50" oeste e altitude de 960 metros. As macieiras silvestres estudadas foram M. atrosanguinea, M. baccata, M. eleyi, M. floribunda, M. hopa, M. platycarpa, M. robusta, 'John Downil', 'Prof. Spengler', 'Milalew imuni', 'Profusion', 'Winter gold' e 'Yellow Siberian'. As espécies silvestres apresentaram grande variabilidade na época de florescimento e na duração do mesmo ao longo dos anos. A maior coincidência do período de floração e com maior regularidade ao longo dos anos foi obtido entre às cultivares Gala e Fuji. 'Prof. Spengler', 'Profusion', 'Winter gold' e 'John Downil' são as espécies silvestres de macieira com maior potencial de utilização como polinizadoras, podendo ser utilizadas complementarmente para polinização das cultivares Gala e Fuji. As espécies M. hopa, M. eleyi e M. atrosanguinea, devido à alta densidade de floração, podem ser utilizadas como segunda opção para a polinização das cultivares Gala e Fuji.The correct choice of pollinator cultivars is an important management factor for obtaining high productivity in apple trees. Many apple production regions in the world adopt wild apple species with the specific purpose of pollination. However, there is little information about the use of this species in the climatic conditions of South Brazil. The objective of this work was to evaluate the

  6. Natural infection by endoparasites among free-living wild animals Infecção natural por endoparasitas em animais silvestres de vida-livre

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    Luciane Holsback

    Full Text Available The objective of this study was to investigate the frequency of occurrence and variety of intestinal parasites among free-living wild animals. Fecal samples from wild mammals and birds at rehabilitation centers in the states of Mato Grosso do Sul and São Paulo were analyzed by sedimentation and flotation-centrifugation methods. Parasite eggs, oocysts, cysts and/or trophozoites were found in 71% of the samples. Cryptosporidium sp. oocysts were detected in fecal samples from oncillas (Leopardus tigrinus and scaly-headed parrots (Pionus maximiliani. Giardia cysts were identified in the feces of a gray brocket (Mazama gouazoubira. Among the most common parasites found, there were eggs from Toxocara cati, Toxascaris leonina and Ancylostoma tubaeforme, and from Cestoda. Several Enterobius sp. eggs were found in the feces of red howler monkeys (Alouatta seniculus. It can be concluded from this study that despite the small number of samples, the diversity of parasites found was noteworthy. Additional information about parasite endofauna in wild animals is needed, since their presence might suggest that there could be proximity to and interactions with domestic animals and/or humans. In addition, further studies on parasites from free-living wild animals are of prime importance for understanding the intensity of anthropic changes in wild environments.O objetivo deste trabalho foi pesquisar a frequência de ocorrência e a variedade de parasitas intestinais de animais silvestres de vida livre. Amostras de fezes de mamíferos e aves silvestres de centros de reabilitação dos Estados do Mato Grosso do Sul e São Paulo, foram analisadas pelos métodos de sedimentação e de centrífugo-flutuação. Foram encontrados ovos, oocistos, cistos e/ou trofozoítos de parasitas em 71% das amostras. Oocistos de Cryptosporidium sp. foram detectados em amostras de fezes de gato-do-mato-pequeno (Leopardus tigrinus e maritacas (Pionus maximiliani. Cistos de Giardia

  7. PO.EX em EXPO (ou POesia EXperimental em EXPOsição: Ernesto Melo e Castro, António Barros e Silvestre Pestana na Casa da Escrita

    Directory of Open Access Journals (Sweden)

    Manaíra Aires Athayde

    2013-07-01

    Full Text Available Review of three exhibitions: Ernesto MELO E CASTRO, Do Leve à Luz (3 october - 2 november 2012; António BARROS, Progestos_Obgestos (30 november - 21 december 2012; Silvestre PESTANA, Povo Novo Virtual (8 february - 1 march 2013. Ciclo NAS ESCRITAS PO.EX, Casa da Escrita, Coimbra, Portugal.

  8. Efecto de la salinidad sobre la fisiología energética del camarón blanco Litopenaeus vannamei (Boone) Effect of salinity on physiological energetics of white shrimp Litopenaeus vannamei (Boone)

    OpenAIRE

    Gustavo Valdez; Fernando Díaz; Ana Denisse Re; Elizabeth Sierra

    2008-01-01

    Se determinó el balance energético en juveniles de Litopenaeus vannamei, aclimatados a la salinidad como hiperosmóticos (20 ups), isosmóticos (26 ups), o hiposmóticos (32 ups). Además, se calculó la razón atómica O:N. La tasa de ingestión, el consumo de oxígeno, la excreción de amonio y el campo de crecimiento fueron afectados significativamente (p < 0.05) cuando los organismos fueron aclimatados a 20, 26 y 32 ups. La mayor cantidad de energía ingerida contenida en el alimento se determinó en...

  9. Pelacakan Virus Bercak Putih pada Udang Vaname (Litopenaeus vannamei di Lombok dengan Real-Time Polymerase Chain Reaction (DETECTION OF WHITE SPOT SYNDROME VIRUS IN LITOPENAEUS VANNAMEI IN LOMBOK ISLAND USING REAL-TIME POLYMERASE CHAIN REACTION

    Directory of Open Access Journals (Sweden)

    Lulu Arafani

    2016-03-01

    Full Text Available White spot syndrome virus (WSSV is one of the most threatening diseases in shrimp and othercrustaceans affecting global shrimp farming. Since firstly detected in Taiwan in 1992, the disease hasspread globally and followed with considerable socio-economic consequences. This research was performedto detect the WSSV infection in shrimp farming in Lombok Island’s (West Nusa Tenggara using real-timepolymerase chain reaction. Samples of vaname (Litopenaeus vannamei were collected from several shrimpfarming in Lombok. Results indicated that the spread of WSSV has reached shrimp farms in Lombok,especially in Lendang Jae, West Lombok. Therefore, a biosurveillance program is strongly recommendedto government to avoid and halt the spread of the disease in East Indonesia region .

  10. BUDI DAYA UDANG VANAMEI (Litopenaeus vannamei DI TAMBAK DENGAN PADAT TEBAR BERBEDA MENGGUNAKAN SISTEM PEMUPUKAN SUSULAN

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    Gunarto Gunarto

    2016-11-01

    Full Text Available Budi daya udang putih, Litopenaeus vannamei dengan sistem teknologi intensif sulit dikembangkan oleh masyarakat petani kecil. Hal ini disebabkan oleh kebutuhan biaya produksi yang sangat tinggi. Untuk itu, teknologi budi daya udang pola tradisional dengan sistem pemupukan susulan perlu dikembangkan. Tujuan penelitian ini adalah untuk mengetahui padat tebar yang optimal pada budi daya udang vanamei pola tradisional plus dengan sistem pemupukan susulan. Penelitian dilaksanakan dengan menggunakan tambak ukuran 500 m2/petak sebanyak 12 petak. Sebelum penebaran dilakukan persiapan tambak meliputi: pemberantasan hama, pengeringan, dan pengapuran. Hewan uji yang digunakan adalah udang vanamei PL 22 dengan padat tebar yang diuji yaitu 1 ekor/m2 (A, 3 ekor/m2 (B, 5 ekor/m2 (C, dan 7 ekor/m2 (D. Masing-masing perlakuan dengan tiga kali ulangan. Dosis pemupukan susulan menggunakan urea dan TSP, sebanyak 750 g urea dan 375 g SP-36/petak, yang diaplikasikan setiap minggu sekali pada bulan pertama pemeliharaan dan setiap dua minggu sekali pada bulan kedua dan ketiga selama pemeliharaan udang dalam tambak. Hasil penelitian menunjukkan bahwa rata-rata ukuran udang hingga umur pemeliharaan 76 hari di tambak telah mencapai ukuran konsumsi (rata-rata 20—21g. Sintasan udang paling tinggi diperoleh pada perlakuan A dan menunjukkan berbeda nyata (P0,05, tetapi ketiga-tiganya menunjukkan perbedaan yang nyata dengan perlakuan C (P0,05 dengan perlakuan B, tetapi berbeda nyata dengan perlakuan C dan A. Pertumbuhan udang di semua perlakuan sangat nyata (P0.05, but there were significantly different (P0.05 with treatment B, but both of them were significantly different (P<0.05 with treatment C and A. Shrimp growth in all treatments most significantly (P<0.01 influenced by salinity, nitrate, ammonium, Total Organic Matter (TOM, and klekap production, while nitrite, nitrate, phosphate, and klekap production are also most  dominant factors (P<0.01 influences to the shrimp

  11. Response of gut health and microbiota to sulfide exposure in Pacific white shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Suo, Yantong; Li, Erchao; Li, Tongyu; Jia, Yongyi; Qin, Jian G; Gu, Zhimin; Chen, Liqiao

    2017-04-01

    Sulfide is a natural and widely distributed toxicant. It can be commonly found on the interface between water and sediment in the aquatic environment. The Pacific white shrimp Litopenaeus vannamei starts life in the benthic zone soon after the mysis stage, an early stage of post larvae. Therefore, L. vannamei is inevitably affected by exposure to sulfide released from pond sediment. This study explored the toxicant effect of different concentrations of sulfide on the intestinal health and microbiota of Pacific white shrimp by monitoring the change of expression of inflammatory, immune related cytokines, and the structure of the intestinal microbiota. The gut histology, expressions of inflammatory and immune related cytokines (tumor necrosis factor-alpha, C-type lectin 3, myostatin and heat shock transcription factor 1), and the microbiota were determined in L. vannamei after exposure to 0 (control), 425.5 (1/10 LC 50-96 h), and 851 μg/L (1/5 LC 50-96 h) of sulfide for 21 days. With the increase of sulfide concentration, intestinal injury was aggravated and the inflammatory and immune related cytokines generated a range of reactions. The expression of myostatin (MSTN) was significantly down-regulated by the concentration of sulfide exposure. No difference in the expression of heat shock transcription factor 1 (HSF1) was found between the control and shrimp exposed to 425.5 μg/L, but significantly higher HSF1 expression was found in shrimp exposed to 851 μg/L of sulfide. Significantly higher values of tumor necrosis factor-alpha (TNF-α) and C-type lectin 3 (CTL3) were found in the shrimp exposed to 425.5 μg/L of sulfide compared to the control, but a lower value was found in the shrimp exposed to 851 μg/L (P Sulfide also changed the intestinal microbial communities. The abundance of pathogenic bacteria, such as Cyanobacteria, Vibrio and Photobacterium, increased significantly with exposure to the increasing concentration of sulfide. The abundance of

  12. Does zero-water discharged technology enhance culture performance of pacific white shrimp (Litopenaeus vannamei Boone.)?

    Science.gov (United States)

    Suantika, Gede; Anggraeni, Jayanty; Hasby, Fahri Azhari; Yanuwiarti, Ni Putu Indah

    2014-03-01

    Litopenaeus vannamei or white leg shrimp is an introduced shrimp which has successfully cultured in Indonesia. In Indonesia, L. vannamei is commonly cultured on outdoor/earthen pond that requires renewal of water, less control in term of water quality and disease and attributed to unpredictable yield production. Based on the existing culture condition, a system that enable to minimize water consumption, improve the hygiene of the culture and at the same time maintain a more stable yield production is urgent to be developed by using a zero water discharge system. The system consists of: (a) culture tank - to retain and culture the shrimp; (b) CaCO3 grained - buffering agent and substrate of nitrifying bacteria; (c) aeration line - to provide O2 and homogenize the culture; (d) ancho (feeding) - to control an appropriate feed; (e) nitrifying bacteria adding - to consume ammonium and nitrite then convert it to nitrate, and also control pathogen Vibrio sp.; (f) diatom microalgae (Chaetoceros gracilis) - to uptake nitrate, bacteriostatic agent, feed source, provide O2 and shading. In this study, there were 2 treatments: the static culture (batch) system was set as control (K) (in 70 PL/m2), and culture system with zero-water discharge system which was inoculated by 0.02% v/v 106 CFU/ml of mixed culture nitrifying bacteria and diatom microalgae in 70 PL/m2 (P1). The white leg shrimp used in this experiment was at post larvae (PL) 10 and cultured in a batch system (1 × 1 × 0.5 m3 pond) during 2 months. Several parameters including survival rate, mean body weight, and water quality (salinity, temperature, pH, DO, ammonium, nitrite, and nitrate) were measured. Based on the results, biomass of P1 (237.12 ± 31.11) gram is significantly higher than control (K) (180.80 ± 12.26) gram (P< 0,05). Water quality during the culture period in all treatments were still in tolerance range of white leg shrimp post larvae, except ammonium concentration in control (K) (2.612 ± 0.56) mg

  13. PENGARUH PENAMBAHAN KALIUM PADA MASA ADAPTASI PENURUNAN SALINITAS TERHADAP PERFORMA PASCALARVA UDANG VANAMEI (Litopenaeus vannamei

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    Ferdinand Hukama Taqwa

    2008-12-01

    Full Text Available Tujuan penelitian ini adalah untuk mengetahui pengaruh penambahan kalium selama masa adaptasi penurunan salinitas terhadap performa pascalarva udang vanamei. Penelitian ini dilaksanakan untuk mendapatkan kadar kalium optimal yang dapat menurunkan tingkat stres dan meningkatkan sintasan pascalarva udang vanamei setelah melalui masa adaptasi penurunan salinitas. Hewan uji yang digunakan adalah PL20 udang vanamei. Rancangan percobaan menggunakan rancangan acak lengkap dengan perlakuan yang diterapkan adalah penambahan kalium ke air tawar pengencer masing-masing sebanyak 0 mg/L (A, 25 mg/L (B, 50 mg/L (C, dan 75 mg/L (D. Penurunan salinitas dilakukan secara gradual selama 4 hari dari salinitas 25 ppt hingga mencapai 2 ppt. Hasil percobaan menunjukkan bahwa penambahan kalium sebanyak 25 mg/L hingga kadar kalium media menjadi 51 mg/L dapat mengurangi pembelanjaan energi untuk osmoregulasi, tingkat stres, dan laju metabolisme standar sehingga meningkatkan sintasan pascalarva udang vanamei setelah melalui masa adaptasi penurunan salinitas selama 96 jam (4 hari. The objective of this research was to study the effect of potassium addition during salinity acclimatization from 25 ppt down to 2 ppt on the performance of Litopenaeus vannamei postlarvae. This experiment was done to determine optimal dosage of potassium which can reduce stress level and increase survival rate of L. vannamei postlarvae after salinity acclimatization. Specimen test used was PL20 of white shrimp (0.001 g. Experimental design used completely randomized design with four treatments and three replications of different potassium addition levels to freshwater: 0 mg/L (A, 25 mg/L (B, 50 mg/L (C, and 75 mg/L (D. Dilution of salinity was done gradually using freshwater during 4 days from 25 ppt down to 2 ppt. The result of this experiment indicated that the addition of 25 mg/L potassium (potassium level in media was 51 mg/L reduced the energy cost for osmoregulation, level of stress and

  14. Dopamine beta-hydroxylase participate in the immunoendocrine responses of hypothermal stressed white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Cheng, Winton; Ka, Ya-Wen; Chang, Chin-Chyuan

    2016-12-01

    Dopamine beta-hydroxylase (DBH) plays a critical role in catecholamine (CA) synthesis of neuroendocrine regulatory network, and is suggested to be involved in the immunoendocrine responses of invertebrate against bacterial challenge. DBH has been identified in white shrimp, Litopenaeus vannamei, and further investigation on its potential function was conducted after hypothermal stress, pharmaceutical inhibition and gene silencing in the present study. Cloned DBH L. vannamei (LvDBH), belonging to the Copper type II, ascorbate-dependent monooxygenases, was characterized by a DOMON domain, a Cu2_monooxygen domain and three glycosylation sites, and its expression was abundant in thoracic ganglia and haemocytes determined by quantitative real-time PCR. The effects of hypothermal stress showed that LvDBH expression in thoracic ganglia, haemocytes and hepatopancreas as well as the DBH contents in haemocytes and dopamine (DA) and norepinephrine (NE) levels in haemolymph are obviously up-regulated. L. vannamei receiving disulfiram for 30-120 min revealed the inhibition of DBH and NE contents in haemocytes and haemolymph respectively, but high level of DA in haemolymph was noticed. Besides, a significant decrease of LvDBH expression in thoracic ganglia, haemocytes and hepatopancreas were also observed. Subsequently, LvDBH expression was successfully silenced in thoracic ganglia, haemocytes and hepatopancreas of shrimp that received LvDBH-dsRNA for 3 days, and meanwhile, a decrease of DBH contents in haemocytes accompanied by decreased levels of NE and DA in haemolymph were also observed. These results indicate that LvDBH possesses the functional domains responsible for CAs synthesis, and therefore, inhibiting DBH contents in haemocytes by disulfiram and by LvDBH-dsRNA resulted in the impaired synthesis of NE from DA in haemolymph. These also suggest that the increased release of DA and NE in haemolymph for potential modulation of physiological or immunological responses

  15. Vaccination Enhances Early Immune Responses in White Shrimp Litopenaeus vannamei after Secondary Exposure to Vibrio alginolyticus

    Science.gov (United States)

    Lin, Yong-Chin; Chen, Jiann-Chu; Morni, Wan Zabidii W.; Putra, Dedi Fazriansyah; Huang, Chien-Lun; Li, Chang-Che; Hsieh, Jen-Fang

    2013-01-01

    Background Recent work suggested that the presence of specific memory or some form of adaptive immunity occurs in insects and shrimp. Hypervariable pattern recognition molecules, known as Down syndrome cell adhesion molecules, are able to mount specific recognition, and immune priming in invertebrates. In the present study, we attempted to understand the immune response pattern of white shrimp Litopenaeus vannamei which received primary (PE) and secondary exposure (SE) to Vibrio alginolyticus. Methodology Immune parameters and proliferation of haematopoietic tissues (HPTs) of shrimp which had received PE and SE to V. alginolyticus were measured. In the PE trial, the immune parameters and proliferation of HPTs of shrimp that received heat-killed V. alginolyticus (HVa) and formalin-inactivated V. alginolyticus (FVa) were measured. Mortality, immune parameters and proliferation of HPTs of 7-day-HVa-PE shrimp (shrimp that received primary exposure to HVa after 7 days) and 7-day-FVa-PE shrimp (shrimp that received primary exposure to FVa after 7 days) following SE to live V. alginolyticus (LVa) were measured. Phagocytic activity and clearance efficiency were examined for the 7∼35-day-HVa-PE and FVa-PE shrimp. Results HVa-receiving shrimp showed an earlier increase in the immune response on day 1, whereas FVa-receiving shrimp showed a late increase in the immune response on day 5. The 7-day-FVa-PE shrimp showed enhancement of immunity when encountering SE to LVa, whereas 7-day-HVa-PE shrimp showed a minor enhancement in immunity. 7-day-FVa-PE shrimp showed higher proliferation and an HPT mitotic index. Both phagocytic activity and clearance maintained higher for both HVa-PE and FVa-PE shrimp after 28 days. Conclusions HVa- and FVa-receiving shrimp showed the bacteria agglutinated prior to being phagocytised. FVa functions as a vaccine, whereas HVa functions as an inducer and can be used as an immune adjuvant. A combined mixture of FVa and HVa can serve as a

  16. Mitigación de daños provocados por fauna silvestre en cultivos agrícolas en un bosque montano de Bolivia

    Directory of Open Access Journals (Sweden)

    Eddy Pérez L.

    2014-12-01

    Full Text Available El daño causado por fauna silvestre en cultivos agrícolas es una de las causas principales de conflicto dentro y fuera de áreas protegidas. Para un adecuado manejo del conflicto se requieren evaluaciones del daño y de la aplicación de distintos métodos de mitigación. El objetivo del trabajo fue evaluar la efectividad de prácticas agroculturales y cacería de control, para reducir daños por vertebrados silvestres en cultivos agrícolas anuales en el PN ANMI Cotapata. Se trabajó en siete parcelas con prácticas agroculturales, cuatro parcelas con cacería de control y cinco parcelas control, registrándose el tipo y densidad de cultivo, frecuencia de visitas de especies dañinas a huelleros, plantación perdida, especies responsables y biomasa de cosecha. Los resultados muestran que las especies más frecuentes fueron: Dasyprocta sp. y Dasypus novemcinctus, siendo estas 1.6 veces más frecuentes en parcelas con prácticas agroculturales. En promedio se perdió 7.3% de la plantación en parcelas agroculturales, 4.6% en parcelas sujetas a cacería de control, y 27.6% en parcelas control. Las principales especies responsables de las pérdidas fueron: P. tajacu, seguido por Dasyprocta sp. y S. apella. A través de estas estrategias de manejo, redujo las pérdidas en >50%, con lo cual se considera que los métodos puestos a prueba son útiles y se recomienda utilizarlos a gran escala con la consideración de que podría ser necesario innovar cada cierto tiempo, pues los animales silvestres tienden a acostumbrarse a los métodos de ahuyentamiento en ejecución.

  17. Effect of inactive yeast cell wall on growth performance, survival rate and immune parameters in Pacific White Shrimp (Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Rutchanee Chotikachinda

    2008-10-01

    Full Text Available Effects of dietary inactive yeast cell wall on growth performance, survival rate, and immune parameters in pacific white shrimp (Litopenaeus vannamei was investigated. Three dosages of inactive yeast cell wall (0, 1, and 2 g kg-1 were tested in three replicate groups of juvenile shrimps with an average initial weight of 7.15±0.05 g for four weeks. There was no significant difference in final weight, survival rate, specific growth rate, feed conversion ratio, feed intake, protein efficiency ratio, and apparent net protein utilization of each treatments. However, different levels of inactive yeast cell wall showed an effect on certain immune parameters (p<0.05. Total hemocyte counts, granular hemocyte count, and bacterial clearance were better in shrimp fed diets supplemented with 1 and 2 g kg-1 inactive yeast cell wall as compared with thecontrol group.

  18. Ammonia exposure induces oxidative stress, endoplasmic reticulum stress and apoptosis in hepatopancreas of pacific white shrimp (Litopenaeus vannamei).

    Science.gov (United States)

    Liang, Zhongxiu; Liu, Rui; Zhao, Depeng; Wang, Lingling; Sun, Mingzhe; Wang, Mengqiang; Song, Linsheng

    2016-07-01

    Ammonia is one of major environmental pollutants in the aquatic system that poses a great threat to the survival of shrimp. In the present study, the mRNA expression of endoplasmic reticulum (ER) stress marker and unfolded protein response (UPR) related genes, as well as the change of redox enzyme and apoptosis were investigated in hepatopancreas of the pacific white shrimp, Litopenaeus vannamei after the exposure of 20 mg L(-1) total ammonia nitrogen (TAN). Compared with the control group, the superoxide dismutase (SOD) activity in hepatopancreas decreased significantly (p vannamei after exposure to ammonia by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. The results indicated that ammonia exposure could induce oxidative stress, which further caused ER stress and apoptosis in hepatopancreas of L. vannamei. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. Digital gene expression analysis in hemocytes of the white shrimp Litopenaeus vannamei in response to low salinity stress.

    Science.gov (United States)

    Zhao, Qun; Pan, Luqing; Ren, Qin; Hu, Dongxu

    2015-02-01

    The white shrimp Litopenaeus vannamei has been greatly impacted by low salinity stress. To gain knowledge on the immune response in L. vannamei under such stress, we investigated digital gene expression (DEG) in L. vannamei hemocytes using the deep-sequencing platform Illumina HiSeq 2000. In total, 38,155 high quality unigenes with average length 770 bp were generated; 145 and 79 genes were identified up- or down-regulated, respectively. Functional categorization and pathways of the differentially expressed genes revealed that immune signaling pathways, cellular immunity, humoral immunity, apoptosis, cellular protein synthesis, lipid transport and energy metabolism were the differentially regulated processes occurring during low salinity stress. These results will provide a resource for subsequent gene expression studies regarding environmental stress and a valuable gene information for a better understanding of immune mechanisms of L. vannamei under low salinity stress. Copyright © 2014 Elsevier Ltd. All rights reserved.

  20. Novel transcriptome assembly and improved annotation of the whiteleg shrimp (Litopenaeus vannamei), a dominant crustacean in global seafood mariculture

    Science.gov (United States)

    Ghaffari, Noushin; Sanchez-Flores, Alejandro; Doan, Ryan; Garcia-Orozco, Karina D.; Chen, Patricia L.; Ochoa-Leyva, Adrian; Lopez-Zavala, Alonso A.; Carrasco, J. Salvador; Hong, Chris; Brieba, Luis G.; Rudiño-Piñera, Enrique; Blood, Philip D.; Sawyer, Jason E.; Johnson, Charles D.; Dindot, Scott V.; Sotelo-Mundo, Rogerio R.; Criscitiello, Michael F.

    2014-01-01

    We present a new transcriptome assembly of the Pacific whiteleg shrimp (Litopenaeus vannamei), the species most farmed for human consumption. Its functional annotation, a substantial improvement over previous ones, is provided freely. RNA-Seq with Illumina HiSeq technology was used to analyze samples extracted from shrimp abdominal muscle, hepatopancreas, gills and pleopods. We used the Trinity and Trinotate software suites for transcriptome assembly and annotation, respectively. The quality of this assembly and the affiliated targeted homology searches greatly enrich the curated transcripts currently available in public databases for this species. Comparison with the model arthropod Daphnia allows some insights into defining characteristics of decapod crustaceans. This large-scale gene discovery gives the broadest depth yet to the annotated transcriptome of this important species and should be of value to ongoing genomics and immunogenetic resistance studies in this shrimp of paramount global economic importance. PMID:25420880

  1. Evaluation of the bioaccumulation of heavy metals in white shrimp (Litopenaeus vannamei) along the Persian Gulf coast.

    Science.gov (United States)

    Dadar, Maryam; Peyghan, Rahim; Memari, Hamid Rajabi

    2014-09-01

    The concentrations of heavy metals in Persian Gulf are low, but petrochemical and refinery activities have caused an increase in heavy metal wastes, especially in coastal regions. The present study was done to determine the bioaccumulation of heavy metals in the muscle of white shrimp (Litopenaeus vannamei) using flame atomic absorption spectrophotometry. The experiment was conducted in four important coastal regions of the Persian Gulf: Bushehr, Deylam, Mahshahr, and Abadan. Amounts of seven heavy metals such as Copper (Cu), Iron (Fe), Lead (Pb), Zinc (Zn), Nickel (Ni), Cadmium (Cd), and Cobalt (Co), were measured as µg/g heavy metal in dry weight in the muscle of white shrimp from the afore-mentioned regions during 2011. This study revealed information that the primary risk for human health and the marine life chain was lead in the muscles of white shrimp in Mahshahr, where intense petrochemical and refinery activities are conducted. Concentrations of other heavy metals were lower than world standards.

  2. Viabilidade de agentes bacterianos como probiÃtico no cultivo do camarÃo marinho Litopenaeus vannamei

    OpenAIRE

    Deborah Oliveira Amarante

    2016-01-01

    Os probiÃticos surgiram como alternativa ao uso de antibiÃticos no controle de bactÃrias patogÃnicas. Buscando alternativas para reduzir as vibrioses surgiu a utilizaÃÃo das bactÃrias do gÃnero Bacillus como prevenÃÃo para o aparecimento de enfermidades. O objetivo principal desta pesquisa foi testar a viabilidade e eficiÃncia de bactÃrias isoladas do trato intestinal de crustÃceos saudÃveis como agentes probiÃticos no cultivo do camarÃo Litopenaeus vannamei. Foram testadas 17 cepas do gÃnero...

  3. A ten-month diseases survey on wild Litopenaeus setiferus (Decapoda: Penaeidae from Southern Gulf of Mexico

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    Rodolfo Enrique del Río-Rodríguez

    2013-09-01

    Full Text Available The development of shrimp aquaculture in Mexican coasts of the Gulf of Mexico began to be explored using the Pacific white shrimp Litopenaeus vannamei in the mid 90´s. Many concerns over the risk of disease transmission to the economically important native penaeids, have been the main deterrent for the aquaculture of L. vannamei in the region. Concurrently, more than 10 years of research experience on the aquaculture suitability of the native Litopenaeus setiferus from the Terminos Lagoon, in the Yucatán Peninsula, have been accumulated. The aim of this study was then to determine the seasonal variations of the naturally acquired diseases and the possible detection of exotic pathogens. For this, random subsamples (n~60 of juveniles L. setiferus were collected from monthly captures. In order to detect the widest range of pathogens, including infectious hypodermal and hematopoietic necrosis (IHHNv and white spot syndrome (WSSv viruses, both histopathological and molecular methods were employed. Monthly prevalence (% was calculated for every finding. We were able to detect a total of 16 distinct histological anomalies, most of which the presump- tive aetiological agent was readily identified. PCR results for viruses were negative. For some pathogens and symbionts, the prevalence was significantly different between the adult and juvenile populations. Prevalence of diseases tended to be higher in juvenile shrimp than in adults. The results of this study indicated that L. setiferus carry a wide variety of pathogens and symbionts that seem to be endemic to penaeids of the Gulf of Mexico, and those juveniles were more conspicuous to acquire pathogens and symbionts than adults.

  4. A ten-month diseases survey on wild Litopenaeus setiferus (Decapoda: Penaeidae) from Southern Gulf of Mexico.

    Science.gov (United States)

    del Río-Rodríguez, Rodolfo Enrique; Pech, Daniel; Soto-Rodriguez, Sonia Araceli; Gomez-Solano, Monica Isela; Sosa-lopez, Atahualpa

    2013-09-01

    The development of shrimp aquaculture in Mexican coasts of the Gulf of Mexico began to be explored using the Pacific white shrimp Litopenaeus vannamei in the mid 90's. Many concerns over the risk of disease transmission to the economically important native penaeids, have been the main deterrent for the aquaculture of L. vannamei in the region. Concurrently, more than 10 years of research experience on the aquaculture suitability of the native Litopenaeus setiferus from the Terminos Lagoon, in the Yucatán Peninsula, have been accumulated. The aim of this study was then to determine the seasonal variations of the naturally acquired diseases and the possible detection of exotic pathogens. For this, random subsamples (n-60) of juveniles L. setiferus were collected from monthly captures. In order to detect the widest range of pathogens, including infectious hypodermal and hematopoietic necrosis (IHHNV) and white spot syndrome (WSSV) viruses, both histopathological and molecular methods were employed. Monthly prevalence (%) was calculated for every finding. We were able to detect a total of 16 distinct histological anomalies, most of which the presumptive aetiological agent was readily identified. PCR results for viruses were negative. For some pathogens and symbionts, the prevalence was significantly different between the adult and juvenile populations. Prevalence of diseases tended to be higher in juvenile shrimp than in adults. The results of this study indicated that L. setiferus carry a wide variety of pathogens and symbionts that seem to be endemic to penaeids of the Gulf of Mexico, and those juveniles were more conspicuous to acquire pathogens and symbionts than adults.

  5. Caracterización morfológica de accesiones silvestres de guayaba Morphologic characterization of wild accesions of guava

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    Liliana Jiménez Lozano

    2009-04-01

    Full Text Available Se realizó la caracterización morfológica de 22 accesiones silvestres de guayaba Psidium guajava L. 14 colectadas en el municipio de Restrepo (Valle del Cauca, seis en Armenia (Quindío y dos en Pereira (Risaralda. Se utilizaron 12 descriptores cuantitativos y 10 cualitativos de tallo, hojas y frutos; el análisis de agrupamiento se hizo mediante el coeficiente de Dice-Nei-Li y el promedio aritmético no ponderado (UPGMA. La mayor variabilidad se halló en los descriptores peso de la pulpa (CV = 55.92%, peso (CV = 45.23%, y acidez del fruto (CV = 44.75%. El análisis de agrupamientos con base en caracteres cuantitativos permitió establecer cuatro grupos: las accesiones del grupo A (Armenia, presentaron valores promedio de los descriptores de la calidad del fruto (grados Brix, acidez del fruto y relación grados Brix/acidez y valores altos de contenido de pulpa. La mayoría de accesiones del grupo C (Restrepo tuvieron altos valores de calidad del fruto y bajo contenido de pulpa. El grupo B, constituido por accesiones de Armenia y Pereira, se diferenció por valores bajos en los descriptores de rendimiento del fruto (peso del fruto, de la pulpa y diámetro de la cavidad seminal. Las accesiones del grupo D (Restrepo mostraron valores promedios en calidad y rendimiento del fruto. Los descriptores cuantitativos se reunieron en tres variables sintéticas para rendimiento y calidad del fruto que representaron 76.86% de la variabilidad total.Quantitative and qualitative morphologic characterization of 22 wild accessions of guava Psidium guajava L. collected in Restrepo (Valle del Cauca, Armenia (Quindío and Pereira (Risaralda was done. Twelve (12 quantitative and ten (10 qualitative descriptors of stem, leaves and fruits were used. The Dice- Nei Li coefficient and the UPGMA was used for the cluster analysis. Dendrograms and principal components analysis were used. The highest variability was associated with fruit descriptors, pulp weight (CV = 55

  6. Reproducción de cochinilla silvestre Dactylopius opuntiae (Homóptera: Dactylopiidae Reproduction of wild cochineal Dactylopius opuntiae (Homoptera: Dactylopiidae

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    Arnoldo Flores-Hernández

    2006-06-01

    Full Text Available Por sus características, la cochinilla silvestre ofrece perspectivas de aprovechamiento como fuente de carmín y para el mejoramiento genético de la grana fina. Por lo anterior, se estableció una multiplicación y crianza de cochinilla silvestre, proveniente de la zona árida del norte de México para caracterizarla, utilizando como hospedero a Opuntia megacantha Salm Dyck. Los resultados indican que la especie silvestre pertenece al género-especie Dactylopius opuntia. Se determinó la presencia de partenogénesis en hembras. La duración de los estadios biológicos depende del sexo del insecto; el adulto hembra permaneció durante 38.4 días y 4.2 días para machos, los primeros estadios ninfales fueron similares en duración (18.1-19.8 días. El ciclo biológico de las hembras fue de 77 días mientras que el de los machos fue de 43 días. Para las hembras se estimó un periodo de preoviposición de 18.8 días, manteniéndose en oviposición durante 21 días con un promedio de 131 insectos por hembra. La proporción sexual hembras: machos fue 1:1. El tipo de reproducción fue predominantemente sexual, aunque hubo hembras partenogénicas. Este es el primer reporte de Dactylopius opuntiae como cochinilla silvestre asociada a la zona árida del noreste de México, específicamente en el Bolsón de Mapimí, Durango, México.Wild cochineal has characteristics that offer advantage as a source of carmine and for fine cochineal improvement. To characterize wild cochineal, we initiated a breeding effort raising wild cochineal from the arid zone of the north oh Mexico, using as a host organism Opuntia megacantha Salm dick. The results indicate that the wild specie is Dactylopius opuntiae. The presence of parthenogenesis in females was determined. The duration of ontogenetic stages depends on the sex of the insect. The adult female lasted 38.4 days and 4.2 days for males, the first stage nymphs were similar in duration (18-19.8 days. The complete

  7. Produção e caracterização de alotetraploides sintéticos entre espécies silvestres do gênero Arachis

    OpenAIRE

    Santos, Silvio Pereira dos

    2013-01-01

    Registros arqueológicos indicam que o amendoim foi domesticado por índios há pelo menos 3.500 anos na América do Sul. O amendoim (Arachis hypogaea L.) pertence à família Fabaceae, subfamília Faboidea. O gênero Arachis é composto por cerca de 80 espécies, todas endêmicas da América do Sul. O Brasil se destaca por reunir todas as secções e a maioria das espécies. As espécies silvestres, mais próximas ao amendoim cultivado são quase todas diploides e são classificadas em genomas A, B e D. O grup...

  8. Epidemiologia da Leptospirose em animais silvestres na Fundação Parque Zoológico de São Paulo

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    Sandra Helena Ramiro Corrêa

    2004-06-01

    Full Text Available A Leptospirose é uma doença bacteriana de caráter zoonótico que afeta os animais domésticos, silvestres e o homem. Levantamentos sorológicos têm demonstrado o envolvimento de diferentes espécies sinantrópicas e silvestres na epidemiologia da doença. Com o objetivo de conhecer melhor a epidemiologia da Leptospirose dentro da Fundação Parque Zoológico de São Paulo, foi realizado um estudo sorológico nos animais silvestres mantidos em cativeiro, no período de 1996 a 1999. Foram colhidas amostras de sangue de 302 animais atendidos na rotina da Divisão de Veterinária, das quais 59 apresentaram resultado positivo (19.5% para a prova de Soroaglutinação Microscópica. Os sorovares mais prováveis para o conjunto total de resultados foram : copenhageni (15/59=25.4%, pomona (13/59=22% e castellonis (10/59=16.9%. Entre os animais silvestres examinados os sorovares mais prováveis foram : Família Callithrichidae : castellonis (3/3=100%, Família cebidae : copenhageni : (13/21=65%, grippotyphosa (2/21=9.5% e castellonis (1/21=44.7%. Família felidae : pomona (12/17=70.5%, icterohaemorrhagiae (2/17=11.7% e grippotyphosa (1/17=5.8%, Família canidae : castellonis (2/4=50%, cynopteri (1/4=25% e mini (1/4=25%, Família cervidae : mini (1/1=100%, Família bovidae : copenhageni (2/3=66.6%, pomona (1/3=33.3%, Família dasyproctidae : castellonis (2/3=66.6%, Família macropodidae: sentot (1/1=100%, família giraffidae : castellonis (1/1=100%. Animais de vida livre como ratos (Rattus norvegicus e gambás (Didelphis marsupialis também foram submetidos a prova de soroaglutinação necroscópica e cultura bacteriológica. Foram encontrados testes positivos para o sorovar icterohaemorrhagiae em 42,8% dos ratos e 40% dos gambás estudados. As freqüências de positivos quando analisadas do ponto de vista da localização espacial dos recintos destes animais, permitiram a verificação da existência de áreas críticas para exposição à leptospira

  9. ESTRATÉGIAS QUE CONTRIBUEM PARA A IDENTIFICAÇÃO E CONTENÇÃO DE SURTO DE FEBRE AMARELA SILVESTRE NO GOIÁS

    OpenAIRE

    Menezes, Thiago Vinicius N. de; Instituto Federal de Educação, Ciência e Tecnologia de Goiás; Pereira, Sirlene d. F

    2010-01-01

    O foco desse estudo foi apresentar ferramentas que proporcionam tanto a identificação como a contenção e controle dos casos de Febre Amarela Silvestre No Goiás. Este estudo teve por base a análise de dados de morbimortalidade por febre amarela e imagens de satélite (geoprocessamento) de pontos do Goiás. Os dados foram disponibilizados pela Secretaria de Vigilância em Saúde/MS, visando o conhecimento das estatísticas relacionadas à doença, das estratégias de controle adotadas nas três instânci...

  10. Viabilidad poblacional de Alouatta palliata (Primates: Atelidae y Cebus capucinus (Primates: Cebidae en el Refugio de Vida Silvestre Privado Nogal, Sarapiquí, Heredia, Costa Rica

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    Jorge Rodríguez-Matamoros

    2012-06-01

    Full Text Available La destrucción del hábitat conlleva a la fragmentación de poblaciones de especies silvestres y se considera como uno de los principales factores en la extinción de especies A medida que las poblaciones se vuelven más pequeñas, surgen amenazas hacia su estabilidad y persistencia, como resultado de factores estocásticos demográficos, ambientales y genéticos. El objetivo de este trabajo fue estudiar los efectos de la fragmentación de poblaciones en la viabilidad de Alouatta palliata y Cebus capucinus en el Refugio de Vida Silvestre Privado Nogal (RVSPN, Sarapiquí, Heredia. Para esto se uso el programa VORTEX para correr un análisis de viabilidad de poblaciones (PVA para ambas especies. La información utilizada en el PVA proviene de la estructura demográafica de las poblaciones del RVSPN, literatura sobre la historia natural de las especies y artículos relacionados con PVA. Los resultados sugieren que tanto A. palliata como C. capucinus pueden sobrevivir en fragmentos boscosos aislados. Sin embargo, si se incorporan factores como depresión por endogamia, catástrofes o perdida de hábitat, las pequeñas poblaciones fragmentadas se vuelven inestables y aumenta el riesgo de que desaparezcan. Las poblaciones continuas fueron más robustas ante las amenazas incorporadas, por lo que se recomienda continuar con la reforestación para unir los fragmentos boscosos. Es importante darle seguimiento a las poblaciones de ambas especies y tener un manejo de su hábitat para disminuir los efectos negativos de diferentes eventos estocásticos provocados por el ambiente.

  11. Tratamiento de efluentes del cultivo de Litopenaeus vannamei mediante procesos de sedimentación, filtración y absorción Treatments of effluents from Litopenaeus vannamei shrimp cultures through sedimentation, filtration and absorption

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    Roberto Ramos

    2010-01-01

    Full Text Available La eficiencia de remoción de materia particulada y nutrientes disueltos de efluentes de estanques de reproductores de Litopenaeus vannamei fue evaluada a escala de laboratorio, empleando tratamientos de sedimentación, filtración por Crassostrea rhizophorae y absorción por Ulva fasciata. En cada tratamiento se empleó un tiempo de residencia hidráulica del efluente de seis horas. En la etapa de sedimentación se utilizaron estanques con 90 L del efluente. Para la etapa de filtración, se emplearon estanques con 20 L del efluente sobrenadante de la etapa de sedimentación. En la absorción se utilizaron estanques con 15 L del efluente sobrenadante de la etapa de filtración. Al final de cada etapa se colectaron muestras de 500 mL para los análisis de calidad de agua. Los resultados indicaron que en la sedimentación, la turbidez, clorofila-a y sólidos suspendidos totales presentaron una remoción de 93,8%, 94,5% y 65,9%, respectivamente. La filtración alcanzó eficiencias de 89,3% y 100% para bacterias totales y clorofila a, respectivamente. En la absorción, fosfato (PO4-3 , amonio (N-NH4- y nitrato (N-NO3 fueron reducidos en 53,6%, 49,6% y 70,2%, respectivamente, en relación a los valores del estanque control. Los tratamientos combinados de sedimentación, filtración y absorción, bacterias totales (95,1%, turbidez (97,1% sólidos suspendidos totales (81,3%, clorofila-a (99,1%, amonio (54,1%, nitrito (58,0%, nitrato (69,2% y fosfato (52,9%, presentaron una alta eficiencia de remoción, en relación a los valores del efluente bruto. Se concluye que la combinación de tratamientos mejora significativamente la calidad de los efluentes del cultivo del camarón Litopenaeus vannamei.

  12. QUALITY OF THE WATER IN SHRIMP FARM Litopenaeus vannamei WITH SYSTEM OF PARTIAL RECIRCULATION SYSTEM. QUALIDADE DA ÁGUA EM FAZENDA DE CAMARÃO MARINHO Litopenaeus vannamei COM SISTEMA DE RECIRCULAÇÃO PARCIAL.

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    Luiz Otávio Brito

    2008-12-01

    Full Text Available The present work aim to characterize the quality of the water affluent and effluent of a shrimp Litopenaeus vannamei farm , which operates with system of partial recirculation system. The samples were accomplished during the low water and high tide periods of the day. The analyzed variables were: dissolved oxygen, temperature, pH, salinity, ammonia, nitrate, nitrite and phosphate. They samples were seven points, distributed strategically in way to characterize the water from the entrance going by the inside of the to your exit farm. ANOVA and Tukey test (P<0,05 were used in statistical analysis. Was observed the pH and dissolved oxygen stayed presenting averages of 7.72 and 6.58mg.L-1, respectively. The final averages went from 1.40 mg.L-1 to ammonia, 0.76 mg.L-1 for nitrate, 0.08 mg.L-1 for nitrite and 1.63 mg.L-1 for the phosphate.

    KEY WORDS: Quality of the water, recirculation, shrimp, vannamei.
    O presente trabalho teve como objetivo caracterizar a qualidade da água afluente e efluente de uma fazenda de camarão marinho Litopenaeus vannamei, que opera com sistema de recirculação parcial de água. As coletas foram realizadas durante os períodos de baixamar e preamar, diurnas, nos dias de lua. Analisaram-se as seguintes variáveis: oxigênio dissolvido, temperatura, pH, salinidade, amônia, nitrato, nitrito e fosfato. Escolheram-se sete pontos de coleta, distribuídos estrategicamente, de modo a caracterizar a água desde a entrada, passando pelo interior da fazenda até sua saída. Para análise estatística, utilizaram-se análise de variância (ANOVA e teste de Tukey (P<0,05. Observou-se que o pH e o oxigênio dissolvido apresentaram uma média de 7,72 e 6,58mg.L-1, respectivamente. As médias finais foram de 1,40mg.L-1 para amônia, 0,76mg.L-1 para nitrato, 0,08mg.L-1 para nitrito e 1,63mg.L-1 para o fosfato.

    PALAVRAS-CHAVES: Camarão, vannamei, qualidade da água, recirculação

  13. Ectoparasitos de roedores e marsupiais no ambiente silvestre de Ilha Grande, Estado do Rio de Janeiro, Brasil Ectoparasites collected from wild mammals of Ilha Grande, state of Rio de Janeiro, Brazil, are listed

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    Neide Guitton

    1986-06-01

    Full Text Available Apresentamos uma lista de ectoparasitos encontrados em mamíferos silvestres de Ilha Grande, Rio de Janeiro.Ectoparasites collected from wild mammals of Ilha Grande, state of Rio de Janeiro, Brazil, are listed. The most frequent species of mammal was Proechimys dimidiatus (Gunther,1877; Gigantolaelaps oudemansi Fonseca, 1939 and polygenis lakoi Guimarães, 1948 were the predominant species of acari and fleas, respectively.

  14. Embriões liofilizados e flocos de Artemia no cultivo pós-larval de Litopenaeus vannamei (Boone, 1931 - DOI: 10.4025/actascibiolsci.v30i1.1424 Use of freeze-dried embryos and Artemia flakes in the post-larval culture of Litopenaeus vannamei (Boone, 1931 - DOI: 10.4025/actascibiolsci.v30i1.1424

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    Pedro Hercílio de Oliveira Cavalcante

    2008-03-01

    Full Text Available O objetivo do estudo foi avaliar o crescimento e sobrevivência de pós-larvas de Litopenaeus vannamei que receberam embriões liofilizados (EMB e flocos (FLO de Artemia como suplementos alimentares a uma dieta microparticulada (DMP. Nos tratamentos T1, T2 e T3, os camarões receberam DMP e após 2h, acréscimos de FLO (T1; de EMB (T2; e da mesma DMP, anteriormente, ofertada (T3. No T4, as pós-larvas foram alimentadas com DMP, mas sem suplementações posteriores. Não houve diferença significativa (p > 0,05 na sobrevivência dos camarões após o período experimental de 21 dias. Por outro lado, o ganho de peso absoluto (mg peso seco das pós-larvas, cuja dieta foi suplementada com BEM, foi significativamente superior (p Artemia e recomendam sua inclusão na dieta pós-larval de Litopenaeus vannameiThe objective of the study was to evaluate the growth and survival of Litopenaeus vannamei post-larvae fed with freeze-dried embryos and Artemia flakes as feeding supplements to a microparticulate diet (DMP. In treatments T1, T2 and T3, the shrimp were fed with DMP and after 2 hours, received supplements of FLO (T1; EMB (T2; and the same DMP previously offered (T3. In T4, post-larvae were fed with DMP but without additional supplementation. There was no significant difference (p > 0.05 in shrimp survival after the experimental period of 21 days. On the other hand, the absolute weight gain (mg of dry weight of post-larvae whose diet was supplemented with EMB (T2 was significantly higher (p Artemia embryos and recommend their inclusion in the post-larval diet of Litopenaeus vannamei

  15. Effect of copper on the growth of shrimps Litopenaeus vannamei: water parameters and copper budget in a recirculating system

    Science.gov (United States)

    Cheng, Bo; Liu, Ying; Yang, Hongsheng; Song, Yi; Li, Xian

    2014-09-01

    Shrimps ( Litopenaeus vannamei) were intensively cultured in a recirculating aquaculture system for 98 days to investigate effects of 0.3 mg/L Cu on its performance, Cu budget, and Cu distribution. Shrimps in Cu-treated systems had greater mean final weight (11.10 vs 10.50 g), body length (107.23 vs 106.42 mm), survival rate (67.80% vs 66.40%), and yield (6.42 vs 5.99 kg/m3), and lower feed conversion ratio (1.20 vs 1.29) than those in control systems but the differences were not significant. Vibrio numbers remained stable (104-106 colony forming units/mL) in the rearing tanks of both control and treated systems. Total ammonium-N, nitrite-N, nitrate-N, pH, chemical oxygen demand, 5-day carbonaceous biochemical oxygen demand, and total suspended solids were similar in controls and treatments. Dissolved Cu concentration in the treated systems decreased from 0.284 to 0.089 mg/L while in the control systems it increased from 0.006 2 to 0.018 mg/L. The main sources of Cu in the treated systems were the artificially added component (75.7% of total input), shrimp feed (21.0%), water (2.06%), and shrimp biomass (1.22%). The major outputs of Cu occurred via the mechanical filter (41.7%), water renewal (15.6%), and draining of the sediment trap (15.1%). The foam fractionator removed only 0.69% of total Cu input. Harvested shrimp biomass accounted for 11.68% of Cu input. The Cu concentration of shrimps in the Cu-treated systems (30.70 mg/kg wet weight) was significantly higher than that in control systems (22.02 mg/kg). Both were below the maximum permissible concentration (50 mg/kg) for Cu in seafood for human consumption in China. Therefore, recirculating systems can be used for commercial on-growing of Litopenaeus vannamei without loss of shrimp quality, even in water polluted by 0.30 mg/L Cu. The mechanical filter is the main route for Cu removal.

  16. Improvement of survival and development of Pacific white shrimp Litopenaeus vannamei larvae by feeding taurine enriched rotifers

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    Dedi Jusadi

    2015-03-01

    Full Text Available ABSTRACTThe objective of the present experiment was to study the most optimum taurine enrichment concentration of rotifers in improving Pacific white shrimp larva Litopenaeus vannamei survival and development. White shrimp larvae at sixth naupliar stage were reared in 12 units of 500 L fibre glass tanks with a stocking density of 125 ind/L. Starting from zoea two stage (Z-2, the larva was provided with rotifers with different taurine enrichment concentration according to the treatments, i.e. 0 mg/L enrichment medium (A, 25 mg/L (B, 50 mg/L(C, and 100 mg/L (D. The results show that different taurine concentration in the enrichment media increased taurine level in rotifers. Furthermore, the administration of taurine enriched rotifers up to 50 mg/L significantly improved larval survival and may accelerate larval development. The experimental results also concluded that a concentration of 50 mg/L is the most optimum taurine enrichment concentration of rotifers for the improvement of white shrimp larval survival and developmental stage.Keywords: taurine, rotifer, white shrimp, enrichmentABSTRAKPenelitian ini bertujuan untuk mengkaji konsentrasi optimum taurin melalui pengayaan pada rotifera terhadap tingkat kelangsungan hidup dan perkembangan stadia larva udang vaname Litopenaeus vannamei. Larva udang vaname stadia naupli-6 dipelihara dalam 12 tangki fiberglass volume 500 L dengan kepadatan 125 ind/L. Dimulai sejak stadia zoea 2 (Z-2 larva diberi rotifera yang diperkaya dengan taurin dengan konsentrasi yang berbeda sesuai dengan perlakuan, yaitu 0 mg/L media pengkaya (A, 25 mg/L (B, 50mg/L (C, dan 100mg/L (D. Hasil penelitian menunjukkan pengayaan taurin pada konsentrasi yang berbeda menyebabkan peningkatan kandungan taurin rotifera. Sementara pemberian rotifera yang diperkaya taurin hingga 50 mg/L meningkatkan kelangsungan hidup dan mempercepat perkembangan stadia larva udang. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa pemberian

  17. Arbovírus Ilheus em aves silvestres (Sporophila caerulescens e Molothrus bonariensis Ilheus arbovirus in wild birds (Sporophila caerulescens and Molothrus bonariensis

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    Luiz Eloy Pereira

    2001-04-01

    Full Text Available OBJETIVO: Relatar o isolamento do vírus Ilheus no Estado de São Paulo e avaliar o seu impacto para a saúde pública. MÉTODOS: O isolamento de vírus foi realizado em camundongos albinos Swiss, a partir de sangue de aves silvestres, capturadas com redes de espera tipo mist net, armadas no nível do solo, no Parque Ecológico do Tietê, São Paulo. A identificação das cepas isoladas foi feita pelos testes de inibição da hemaglutinação, fixação de complemento e neutralização em camundongos. Amostras de plasma de aves e de mamíferos silvestres foram submetidas à pesquisa sorológica para detecção de anticorpos inibidores de hemaglutinação. RESULTADOS: Foram isoladas duas cepas do vírus Ilheus em sangue de aves das espécies Sporophila caerulescens e Molothrus bonariensis e detectados anticorpos em aves das espécies Columbina talpacoti, Geopelia cuneata, Molothrus bonariensis e Sicalis flaveola, em sagüis das espécies Callithrix jacchus e Callithrix penicillata e no quati Nasua nasua. CONCLUSÕES: O isolamento do vírus Ilheus e a detecção de anticorpos específicos em aves residentes, migratórias e de cativeiro, em sagüis e quatis, comprovam a presença desse agente no Parque Ecológico do Tietê. O comportamento migratório de aves silvestres pode determinar a introdução do vírus em outras regiões. Considerando-se a patogenicidade para o homem e a confirmação da circulação desse agente viral em área urbana, freqüentada para atividade de lazer e de educação, o risco de ocorrência de infecção na população humana não pode ser descartado.OBJECTIVE: To report the first Ilheus arboviruses isolated from wild birds and analyze its public health impact. METHODS: Wild birds and mammals were captured using mist nets and Tomahawk traps, respectively. Blood samples were drawn from these animals and inoculated intracerebrally in Swiss suckling mice found in the Parque Ecológico do Tietê, Brazil. The isolates were

  18. Antioxidant Capacity, Anthocyanins, and Total Phenols of Wild and Cultivated Berries in Chile Capacidad Antioxidante, Antocianinas y Fenoles Totales de Berries Silvestres y Cultivados en Chile

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    Jaime Guerrero C

    2010-12-01

    ón fue determinar la capacidad antioxidante (AC, antocianinas totales (TA y el contenido de fenoles totales (TP, de berries silvestres y cultivados en diferentes localidades de la Región de La Araucanía y Región de Los Ríos (Chile. Para evaluar estos parámetros se utilizó el método de radicales libres 2,2-difenil-2- picrilhidrazilo (DPPH, pH diferencial y Folin-Ciocalteu, respectivamente. Los porcentajes de decoloración del DPPH estuvieron comprendidos entre 67,8% para zarzaparrilla roja y 95,3% para rosa mosqueta. El maqui presentó un contenido de AT significativamente mayor (2240,2 y 1445,3 mg L-1 cianidina 3-glucósido que el resto de los berries, el promedio de AT fue de 335,5 mg L-1. En cuanto al contenido FT los mayores niveles se obtuvieron en los dos cultivares de saskatoon (773,9 y 1001,9 mg L-1 ácido gálico y en rosa mosqueta silvestre (1457,0 y 1140,4 mg L-1 de ácido gálico. En este estudio se concluye que existen diferencias significativas en la capacidad antioxidante de frutos silvestres y cultivados en Chile, observándose una fuerte correlación entre el CA y el contenido de FT.

  19. Divergência genética em germoplasma de aveias silvestres com base em caracteres multicategóricos e quantitativos

    Directory of Open Access Journals (Sweden)

    Simone Meredith Scheffer-Basso

    2012-10-01

    Full Text Available As aveias silvestres são importantes fontes de genes para programas de melhoramento e sua caracterização é fundamental para a efetiva conservação e uso. Por isso, o objetivo deste estudo foi avaliar a divergência genética em uma coleção de 71 subamostras de aveias silvestres, do Banco de Germoplasma da Embrapa Trigo, com base em caracteres multicategóricos e quantitativos. Procederam-se às análises de variância, para os caracteres quantitativos, e multivariada, para ambos os tipos de caracteres. Os métodos de agrupamento UPGMA, a partir da distância euclidiana média (caracteres multicategóricos, e de ligação completa, com base na distância de Mahalanobis (caracteres quantitativos, foram os mais adequados para ilustrar a relação entre as subamostras. A pilosidade da base dos grãos foi o caractere com maior contribuição relativa para divergência genética (32,16% e a menor contribuição foi da pilosidade do nó superior (0,081%. As subamostras divergiram quanto a vinte caracteres: pilosidade da bainha da folha inferior, bordas da lâmina imediatamente abaixo da folha bandeira, nó superior, face externa do lema e base do grão; posição da folha bandeira e das ramificações na panícula; frequência de plantas com folha bandeira recurvada; intensidade da pilosidade do nó superior e da cerosidade do lema; orientação das ramificações na panícula; comprimento dos pelos basais do grão, ráquila, panícula, glumas e planta; cor do lema, tipo de arista, número de grãos por espigueta e ciclo. O germoplasma apresenta elevada variabilidade genética e genes de interesse para o melhoramento de aveias.

  20. DISPONIBILIDAD DE RAÍCES ADVENTICIAS DE TRES ESPECIES SILVESTRES COSECHADAS PARA LA ELABORACIÓN DE ARTESANÍAS EN SANTA ELENA, ANTIOQUIA (COLOMBIA

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    ANA MARÍA BENAVIDES

    2015-01-01

    Full Text Available En los Andes colombianos, un gran número de artesanías se fabrican a partir de raíces adventicias de plantas nativas que crecen de forma silvestre. Sin embargo, la deforestación y las prácticas no sustentables han diezmado la disponibilidad de plantas silvestres productoras de fibra. En este estudio evaluamos la disponibilidad de fibras de tres especies en Santa Elena (Antioquia, Colombia: Asplundia sarmentosa, Philodendron aff. sagittifolium y Clusia multiflora. Después de una búsqueda exhaustiva en la zona de estudio ubicamos menos de 30 individuos adultos de cada especie. Para cada planta se midieron las variables asociadas al tamaño de la planta y se registró el número y la longitud de raíces maduras e inmaduras. Se pesaron fragmentos de raíces para estimar la biomasa. El crecimiento de raíces de cada planta fue monitoreado mensualmente durante siete meses. La disponibilidad actual de fibra (raíces maduras es de ca. 3 352 m (Asplundia 1 481 m, Clusia de 952 m, Philodendron 918 m, con esta cantidad de material se pueden elaborar entre 419 a 838 canastos. De acuerdo a la tasa de crecimiento mensual, una nueva raíz podría alcanzar un tamaño para ser cosechado entre el mes 38 y 71. Plantas más grandes tienen mayor cantidad de raíces maduras, sin embargo, la tasa de crecimiento no está asociada al tamaño de la planta. Nuestros resultados proveen evidencia de que las plantas en Santa Elena presentan una relación (tamaño de la planta, número de raíces maduras que es similar a plantas que se encuentran en zonas no intervenidas, no obstante, la cosecha de fibras en Santa Elena no es una actividad sostenible debido al bajo número de individuos susceptibles de ser cosechados, la lenta tasa de crecimiento de raíces y la alta demanda de fibras. Es necesario implementar estrategias como el enriquecimiento del bosque con estas tres especies para garantizar una actividad sustentable para recolectores y artesanos en Santa Elena y

  1. Evaluación de la resistencia a Prodiplosis longifila Gagné (Diptera: Cecidomyiidae en genotipos de tomate cultivados y silvestres

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    Yuri Mercedes Mena Pérez

    2014-04-01

    Full Text Available Se evaluó la resistencia a Prodiplosis longifila Gagné en tres accesiones silvestres de Solanum habrochaites var. glabratum (PI-134417, PI-134418 y PI-126449 y los materiales avanzados en mejoramiento genético para resistencia al pasador del fruto Neoleucinodes elegantalis: tres genotipos de retrocruzamiento 3: RC3(3143P4, 3247P4, 36110P3 y un genotipo de retrocruzamiento 4: RC4(4212P4, como testigo susceptible se incluyó la variedad Solanum lycopersicum cv. Unapal-Maravilla. Las evaluaciones se realizaron en condiciones de campo y de casa de malla, utilizando un diseño de bloques completos al azar con cuatro repeticiones. Se evaluó el número de brotes sanos/planta, número de brotes con daño por P. longifila, número de brotes con presencia de larvas vivas y muertas del insecto, y número de larvas vivas o muertas por brote. Durante el experimento en campo las poblaciones de P. longifila fueron 0.2 larvas vivas/brote y 4.17% de daño, lo cual no permitió el establecimiento de diferencias entre los genotipos estudiados. En casa de malla, las poblaciones del insecto fueron elevadas, sin embargo las accesiones silvestres no mostraron daño ni fueron preferidas para la oviposición, presentando algún grado de resistencia. Unapal-Maravilla y los genotipos correspondientes a la RC3, fueron susceptibles al insecto. El genotipo 4212P4 mostró el menor daño de las poblaciones segregantes con solo 5.36% de brotes dañados, siendo considerado como de menor preferencia por el insecto plaga. Los genotipos provenientes de retrocruzamientos evaluados en estos ensayos fueron previamente seleccionados por resistencia a N. elegantalis, y en este procedimiento es posible que hayan podido descartarse genotipos con resistencia a P. longifila.

  2. Prevalência da infecção pelo Toxoplasma gondii em animais domésticos, silvestres e grupamentos humanos da Amazônia

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    José João Ferraroni

    1980-01-01

    Full Text Available Através de Reação de Hemaglutinação Indireta para toxoplasmose foram examinadas amostras de sangue de dez diferentes espécies de animais domésticos e silvestres, de um grupamento humano da cidade de Manaus-Amazonas e de um grupamento humano indígena de área distante, no território de Roraima. Em 108 animais domésticos, o exame sorológico foi reagente em 90,6% dos gatos (Felis catus, 68,4% dos cães (Canis familiaris, 60,0% dos bovinos (Bos sp, 41,2% dos galináceos (Gallus sp e 40,0% dos palmípedes (Cairina sp. Nos 104 animais silvestres foram reagentes 75,0% dos felídeos (Felis sp, 63,6% dos marsupiais (Didelphis marsupialis e Marmosa sp, 63,3% dos primatas (Saimiri sp e 61,1% dos roedores (Proechimys. Entre os dois grupos humanos a prevalência foi de 70,6% nos 51 habitantes da área de Manaus, 64,8% nos 37 silvícolas de Roraima. Os autores discutem os resultados obtidos, assim como os diversos aspectos envolvidos na epidemiologia da toxoplasmose e chamam a atenção para a existência de mecanismos de transmissão ainda não esclarecidos, enfatizando a necessidade de maiores estudos dessa zoonose.Serological examination for Toxoplasma gondii in human blood samples and in blood samples from ten different species of animals obtained in Manaus, State of Amazonas-Brazil, are compared with results obtained from similar examinations of blood from humans living in other areas of the Amazon basin. The domestic cat (Felis catus showed the highest levels of antibody for Toxoplasma gondii, whereas the domestic chicken (Gallus domesticus and duck (Cairina sp the lowest. The other animals showed similar intermediate levels of antibody to this protozoa. The authors discuss the results and several aspects of the involvement in epidemiology of toxoplasmosis and call attention to some transmission mechanisms not yet elucidated.

  3. Caracterización preliminar de los invertebrados bentónicos capturados accidentalmente en la pesca de camarones en el norte del estado de Río de Janeiro, sudeste de Brasil Preliminary characterization of benthic invertebrates caught as by-catch in the shrimp fishery in the north of the Rio de Janeiro State, southeastern Brazil

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    Igor David da Costa

    2009-01-01

    Full Text Available Para caracterizar la biodiversidad de invertebrados bentónicos que componen la fauna asociada a la pesca de camarones en el puerto del Farol de Sao Thomé, costa norte del estado de Río de Janeiro, se realizaron 11 pescas mensuales en el año 2004 con redes de arrastre de fondo, cuya área de operaciones comprende 3-5 mn desde la línea de costa, entre 22°00'S y 22°20'S. Los datos registrados de cada taxon y/o especie se refieren a la frecuencia de ocurrencia, frecuencia numérica, biomasa, índice de Importancia Relativa y abundancia. En total se registraron 27 especies de invertebrados bentónicos de Porifera, Cnidaria, Mollusca, Annelida, Crustácea, Echinodermata y Bryozoa. Crustácea fue el más representativo, tanto en número de ejemplares de Petrochirus diogenes, Hepatus pudibundus y Callinectes ornatos, como en biomasa de P. diogenes y H. pudibundas. En términos de frecuencia de ocurrencia en los muéstreos, 11 especies (40,7% fueron constantes; 6 (22,2% accesorias y 10 (37,0% accidentales.In order to characterize the biodiversity of the benthic invertebrate by-catch associated with the shrimp fishery at Farol de Sao Thome harbor, northern Rio de Janeiro State, Brazil, in 2004, 11 monthly trawls were conducted using bottom trawl nets between 22°00'S and 22°20'S and from 3 to 5 nm from the shoreline. The analyzed data for each talon and/or species include frequency of occurrence, numeric frequency, biomass, index of Relative Importance, and abundance. In total, 27 benthic invertebrate species were recorded, including Peripheral, Cnidarians, Mollusk, Annelid, Crustacea, Echinodermata, and Bryozoa. The most representative group was Crustacea, both in number of specimens (Petrochirus diogenes, Hepatus pudibundus, Callinectes ornatus and in biomass (P. diogenes, H. pudibundus. In terms of the frequency of occurrence in the samples, 11 species (40.7% were constant, 6 species (22.2% were accessories, and 10 species (37.0% were by-catch.

  4. Cyanobacteria Community Dynamics and Trophic Status of Intensive Shrimp (Litopenaeus vannamei Farming Pond in Situbondo East Java Indonesia

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    Dian Aliviyanti

    2017-09-01

    Full Text Available The objective of this study was to analyze the dynamics community structure of Cyanobacteria and trophic status in ponds of intensive shrimp culture (Litopenaeus vannamei in Situbondo based on Trophic Diatom Index (TDI.  The ex post facto research was conducted in situ in the hamlet of Pond Mutiara Mas III Klatakan Situbondo East Java Indonesia.  Observation of Cyanobacteria and Diatoms community structure were done every week during four cycles of shrimp farming ponds.  Cycle of shrimp farming ponds is the times for enlargement process of the shrimps from seed to mature which ranges from 90-120 days.  The dependent variables were the density of Cyanobacteria and Diatoms community, as well as chemical parameters nitrite and orthophosphate.  Trophic status was determined from TDI be counted from data of diatom density.  All of the data were then classified using cluster and biplot analysis program PAST Ver. 3.11 to discribe the profile of ecosystem quality.  The research results showed that there were four taxa of Cyanobacteria during farming cycle which the highest density was found from the genus of Oscillatoria.  Based on the value of TDI, we found that the trophic status of water in shrimps pond during the production process was eutrophic until hyper-eutrophic.  The water quality was decreases along with the length of incubation time.

  5. Biotransformation enzyme activities and phase I metabolites analysis in Litopenaeus vannamei following intramuscular administration of T-2 toxin.

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    Deng, Yijia; Wang, Yaling; Sun, Lijun; Lu, Pengli; Wang, Rundong; Ye, Lin; Xu, Defeng; Ye, Riying; Liu, Ying; Bi, Siyuan; Gooneratne, Ravi

    2018-01-01

    T-2 toxin (T-2) is a type-A trichothecene produced by Fusarium that causes toxicity to animals. T-2 contamination of grain-based aquatic feed is a concern for the industries related to edible aquatic crustacean species such as the shrimp industry because it can lead to serious food safety issues. T-2, its metabolites, and selected phase I (EROD, CarE) and phase II (GST, UGT, SULT) detoxification enzymes in hemolymph and tissues were monitored at 0, 5, 10 15, 30, 45, and 60 min following T-2 intramuscular administration (3 mg/kg bw) in shrimp (Litopenaeus vannamei). Marked increases of EROD activity in hepatopancreas and CarE activity in hemolymph, gill, hepatopancreas and intestine were observed followed by increases in phase II enzymes (GST, UGT, SULT) in hepatopancreas, hemolymph, intestine and gill, which remained elevated for an extended period. Time-dependent decrease in shrimp tissue T-2 concentration was observed. HT-2 increased up to 15 min. Most other T-2 metabolites were detected but not T-2 tetraol. Enzyme responses on exposure to T-2 were tissue-specific and time-dependent. Detection results indicated that HT-2 may not be the only important metabolite in aquatic crustacean species. Further investigation into T-2 metabolite toxicity is needed to fully understand the food safety issues related to T-2.

  6. C-Terminal Domain of Hemocyanin, a Major Antimicrobial Protein from Litopenaeus vannamei: Structural Homology with Immunoglobulins and Molecular Diversity

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    Yue-Ling Zhang

    2017-06-01

    Full Text Available Invertebrates rely heavily on immune-like molecules with highly diversified variability so as to counteract infections. However, the mechanisms and the relationship between this variability and functionalities are not well understood. Here, we showed that the C-terminal domain of hemocyanin (HMC from shrimp Litopenaeus vannamei contained an evolutionary conserved domain with highly variable genetic sequence, which is structurally homologous to immunoglobulin (Ig. This domain is responsible for recognizing and binding to bacteria or red blood cells, initiating agglutination and hemolysis. Furthermore, when HMC is separated into three fractions using anti-human IgM, IgG, or IgA, the subpopulation, which reacted with anti-human IgM (HMC-M, showed the most significant antimicrobial activity. The high potency of HMC-M is a consequence of glycosylation, as it contains high abundance of α-d-mannose relative to α-d-glucose and N-acetyl-d-galactosamine. Thus, the removal of these glycans abolished the antimicrobial activity of HMC-M. Our results present a comprehensive investigation of the role of HMC in fighting against infections through genetic variability and epigenetic modification.

  7. Evaluation of dietary soybean meal as fish meal replacer for juvenile whiteleg shrimp, Litopenaeus vannamei reared in biofloc system

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    Hyeonho Yun

    2017-01-01

    Full Text Available Abstract Different levels of dietary soybean meal (SBM as a fish meal (FM replacer, with and without amino acid supplementation, for whiteleg shrimp, Litopenaeus vannamei reared in the biofloc system was examined in eight weeks of feeding trial. Eight experimental diets consisted of a basal diet with 0% FM replacement by SBM provided in clear sea water without biofloc system (S0SW, four diets replacing FM at 0% (S0, 33% (S33, 67% (S67 and 100% (S100 by SBM, and three diets replacing FM at 33% (S33A, 67% (S67A and 100% (S100A by SBM supplemented with amino acids (methionine and lysine in the seawater biofloc system. Results of water quality analyses showed significantly lower total suspended solids and nitrate for S0SW group than all other treatments. Diets S0 and S33A resulted in higher weight gain and specific growth rate among all groups, with no significant differences with S33 group. In addition, whole-body protein and amino acid compositions of shrimp fed S0SW were lower than most biofloc groups. Haemolymph parameters showed significant differences in total protein, cholesterol and triglyceride between groups S0 and S0SW. Also, superoxide dismutase activity showed a decreasing trend with increasing replacement level. In conclusion, based on these results, SBM could replace up to 33% of FM with or without amino acid supplementation in juvenile whiteleg shrimp diets reared in the biofloc system.

  8. Dampak Stres Salinitas Terhadap Prevalensi White Spot Syndrome Virus (WSSV dan Survival Rate Udang Vannamei (Litopenaeus vannamei Pada Kondisi Terkontrol

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    Attabik Mukhammad Amrillah

    2015-08-01

    Full Text Available White spot syndrome (WSS adalah penyakit yang secara signifikan menyebabkan tingginya mortalitas dan kerusakan parah pada budidaya udang. Penelitian ini bertujuan mengetahui dampak stres salinitas terhadap prevalensi WSSV dan survival rate udang vannamei (Litopenaeus vannamei. Penelitian ini menggunakan udang vannamei ukuran PL 40 yang diinfeksi WSSV dengan konsentrasi virus 20 μg/ml pada tiga rentang salinitas yang berbeda 0-10 ppt, 11-20 ppt, 21–30 ppt dan di rendam selama 4 jam kemudian dilakukan pengamatan selama 7 hari pasca infeksi dan diukur survival rate dan kuaitas airnya. Hasil pengamatan menunjukkan bahwa seluruh sampel terinfeksi oleh WSSV, ditunjukkan hasil analisa PCR dan gejala klinis yang timbul. Salinitas 0-10 ppt memberikan hasil persentase survival rate terendah jika dibandingkan dengan rentang salinitas yang lainnya yaitu sebesar 7 ekor atau 33% dari jumlah total sampel yang digunakan. Persentase survival rate udang tertinggi pasca infeksi virus WSSV terdapat pada perlakuan salinitas 21-30 ppt yaitu sebesar 13 ekor atau 63% dari jumlah total individu, dan salinitas 11-20 ppt memiliki persentase survival rate medium yaitu sebesar 10 ekor atau 49% dari jumlah total sampel. Stres salinitas mempengaruhi prevalensi WSSV dengan semakin tingginya tingkat infeksi seiring menurunnya rentang salinitas, akan tetapi survival rate semakin tinggi seiring dengan bertambahnya rentang salinitas.

  9. In vitro digestibility of specific dsRNA by enzymes of digestive tract of shrimp Litopenaeus vannamei

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    Ana R Álvarez-Sánchez

    2017-05-01

    Full Text Available Objective. The digestibility of specific dsRNA by action of the enzymes of digestive tract of the whiteleg shrimp Litopenaeus vannamei was determined in vitro. Materials and methods. Digestive enzyme activity (amylase, lipase, protease, DNase and RNase was measured in the stomach, digestive gland, and anterior, middle, and posterior intestine of juvenile shrimp, and the digestibility of DNA, RNA and the dsRNA-ORF89, specific to WSSV, was determined by in vitro assays, as well as electrophoretic and densitometric analyses. Results. The highest enzymatic activity was found in the digestive gland: amylase (81.41%, lipase (92.60%, protease (78.20%, DNase (90.85%, and RNase (93.14%. The highest digestive capacity against DNA, RNA, and dsRNA was found in the digestive gland (5.11 ng of DNA per minute, 8.55 ng of RNA per minute, and 1.48 ng dsRNA per minute. Conclusions. The highest digestibility of dsRNA-ORF89, specific to WSSV, was found in the digestive gland, whereas the lowest digestibility was observed in the posterior intestine. This is the first report regarding the digestibility of dsRNA-ORF89 by whiteleg shrimp digestive tract enzymes, with potential therapeutic importance in shrimp culture to prevent WSSV disease through balanced feed.

  10. INFLUENCE OF FISH MEAL REDUCTION, ALGAE ADDITION, AND ENZYME USE IN SHRIMP FEEDS ON THE TISSUE COMPOSITION OF Litopenaeus vannamei

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    Sebastian Kaspers

    2012-06-01

    Full Text Available At the Leibniz-Center for Tropical Marine Ecology in Bremen, Germany, shrimps (Litopenaeus vannamei fed feeds with reduced fish meal content, algae and enzyme additions were analysed for their tissue composition. Contents of protein, glycogen, total lipid, linoleic acid (LA, alpha linolenic acid (ALA, eicosapentaenoic acid (EPA and docosahexaenoic acid (DHA of the shrimp abdomen and of the respective feeds were determined. Reduction of fish meal (FM content in shrimp feed and its substitution with “mash” (grain distillery waste-”Schlempe”, corn gluten, pea or coarse colza meal did not influence the tissue composition. Additional inclusion of an algae mixture out of Spirulina spp., Phaeodactylum spp., and Tetraselmis spp. to shrimp feeds as well as the single inclusions of Phaeodactylum spp. or Spirulina spp. had no effect on the tissue composition. Furthermore no improvement of the quality of the shrimps due to an enhanced digestibility of mash by enzymes (mixture of corolase, phytase, xylanase could be detected. In contrast the quality of shrimps seemed to be negatively affected by enzymes in this study. These findings could contribute to making shrimp aqua farming more economic as reduction of feed costs without reduction of the quality of the shrimps would be a large benefit.

  11. Transcriptome analysis on the exoskeleton formation in early developmetal stages and reconstruction scenario in growth-moulting in Litopenaeus vannamei.

    Science.gov (United States)

    Gao, Yi; Wei, Jiankai; Yuan, Jianbo; Zhang, Xiaojun; Li, Fuhua; Xiang, Jianhai

    2017-04-24

    Exoskeleton construction is an important issue in shrimp. To better understand the molecular mechanism of exoskeleton formation, development and reconstruction, the transcriptome of the entire developmental process in Litopenaeus vannamei, including nine early developmental stages and eight adult-moulting stages, was sequenced and analysed using Illumina RNA-seq technology. A total of 117,539 unigenes were obtained, with 41.2% unigenes predicting the full-length coding sequence. Gene Ontology, Clusters of Orthologous Group (COG), the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and functional annotation of all unigenes gave a better understanding of the exoskeleton developmental process in L. vannamei. As a result, more than six hundred unigenes related to exoskeleton development were identified both in the early developmental stages and adult-moulting. A cascade of sequential expression events of exoskeleton-related genes were summarized, including exoskeleton formation, regulation, synthesis, degradation, mineral absorption/reabsorption, calcification and hardening. This new insight on major transcriptional events provide a deep understanding for exoskeleton formation and reconstruction in L. vannamei. In conclusion, this is the first study that characterized the integrated transcriptomic profiles cover the entire exoskeleton development from zygote to adult-moulting in a crustacean, and these findings will serve as significant references for exoskeleton developmental biology and aquaculture research.

  12. Effect of culture intensity and probiotics application on microbiological and environmental parameters in Litopenaeus vannamei culture ponds.

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    Patil, Prasanna Kumar; Muralidhar, M; Solanki, Haresh G; Patel, Pretesh P; Patel, Krishna; Gopla, Chavali

    2016-01-01

    The present study examined the complex interaction among stocking density and extent of probiotic use with production and environmental parameters in Litopenaeus vannamei culture ponds to suggest suitable management strategies. The study was conducted inL. vannamei culture ponds with stocking density of 35 nos sq m(-1) (Group I) and 56 nos sq m(-1) (Group II) and probiotic application @16.5 kg ha(-1) and 157 kg ha(-1), respectively. There was no significant difference noted between the two groups of ponds in respect to ammonia oxidizing bacteria (AOB) in sediment and nitrite oxidizing bacteria (NOB) in water samples, whereas significantly higher levels of AOB in water samples of high intensity culture ponds (Group II) and NOB in sediment samples of Group I were observed. The levels of sulphur oxidizing bacteria (SOB) and sulphur reducing bacteria (SRB) in Group I pond water and in Group II sediment were significantly higher than their corresponding levels in the other group. In both the groups, ammonia, nitrite and sulphide concentrations were below toxic limits prescribed for shrimp farming. Comparing the production parameters at harvest revealed that low intensity culture ponds (Group I) had higher growth rate, average body weight and significantly lower FCR and higher survival rate than high intensity culture ponds (Group II). The results indicated that application of microbial products in higher quantities did not benefit significantly, and there is a need to regulate quantum and schedule of biological product usage for economically sustainable shrimp culture.

  13. Transcriptomic analyses on muscle tissues of Litopenaeus vannamei provide the first profile insight into the response to low temperature stress.

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    Wen Huang

    Full Text Available The Pacific white shrimp (Litopenaeus vannamei is an important cultured crustacean species worldwide. However, little is known about the molecular mechanism of this species involved in the response to cold stress. In this study, four separate RNA-Seq libraries of L. vannamei were generated from 13°C stress and control temperature. Total 29,662 of Unigenes and overall of 19,619 annotated genes were obtained. Three comparisons were carried out among the four libraries, in which 72 of the top 20% of differentially-expressed genes were obtained, 15 GO and 5 KEGG temperature-sensitive pathways were fished out. Catalytic activity (GO: 0003824 and Metabolic pathways (ko01100 were the most annotated GO and KEGG pathways in response to cold stress, respectively. In addition, Calcium, MAPK cascade, Transcription factor and Serine/threonine-protein kinase signal pathway were picked out and clustered. Serine/threonine-protein kinase signal pathway might play more important roles in cold adaptation, while other three signal pathway were not widely transcribed. Our results had summarized the differentially-expressed genes and suggested the major important signaling pathways and related genes. These findings provide the first profile insight into the molecular basis of L. vannamei response to cold stress.

  14. Identification and function of leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2 in Litopenaeus vannamei.

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    Shuang Zhang

    Full Text Available Leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2 is a myeloid differentiation factor 88-interacting protein with a positive regulatory function in toll-like receptor signaling. In this study, seven LRRFIP2 protein variants (LvLRRFIP2A-G were identified in Litopenaeus vannamei. All the seven LvLRRFIP2 protein variants encode proteins with a DUF2051 domain. LvLRRFIP2s were upregulated in hemocytes after challenged with lipopolysaccharide, poly I:C, CpG-ODN2006, Vibrio parahaemolyticus, Staphylococcus aureus, and white spot syndrome virus (WSSV. Dual-luciferase reporter assays in Drosophila Schneider 2 cells revealed that LvLRRFIP2 activates the promoters of Drosophila and shrimp AMP genes. The knockdown of LvLRRFIP2 by RNA interference resulted in higher cumulative mortality of L. vannamei upon V. parahaemolyticus but not S. aureus and WSSV infections. The expression of L. vannamei AMP genes were reduced by dsLvLRRFIP2 interference. These results indicate that LvLRRFIP2 has an important function in antibacterials via the regulation of AMP gene expression.

  15. Daily cycle and body characteristics of mating Litopenaeus vannamei shrimps (Decapoda: Penaeidae) in the wild off southern Sinaloa, Mexico.

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    Calderón-Pérez, J A; Rendón-Rodríguez, S; Solís-Ibarra, R

    2007-03-01

    Mating behavior has been described for very few species of penaeoid shrimps. We describe some of the environmental conditions under which mating of Litopenaeus vannamei took place in the ocean, as inferred by the presence of attached spermatophores on the thelycum of females, combined with the presence of mature males with empty ampulla terminalis, both evidence of recent copulation. Out of a total of twelve 24 hr samplings on board the research vessel B/O "EL PUMA", one was selected to examine mating. There were four periods of observation. The highest frequency of females with attached spermatophores were found during the daytime whilst the lowest was registered during the night. Females with attached spermatophore were mostly at stages IV and V of ovarian maturity and fell within the 30-50 mm of C.L. range. Copulating male size ranged between 30 and 40 mm of C.L. and there was a close relation between the percentage of mating females and males. Male to female ratio varied throughout the sampling period but it was never 1:1. The female mean size was, in all cases, bigger than the male mean size. Among the environmental factors, salinity and oxygen varied very slightly throughout the sampling period; temperature variation was more pronounced and those changes were attributed to the tidal oscillation.

  16. Effect of hemoglobin powder substituted for fishmeal on growth performance, protein digestibility, and trypsin gene expression in Litopenaeus vannamei

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    Duangrat Chookird

    2010-05-01

    Full Text Available Recent increases in the price and demand of fishmeal, the primary protein source in shrimp feeds, have caused asearch for alternative protein sources. Hemoglobin powder (HE is a by-product produced by separating hemoglobin fromplasma of farm animal un-coagulant blood. HE contains high protein content but low lipid content, and thus has highpotential for fishmeal substitution.A six week feeding trial was carried out to investigate effects of HE substituted for fishmeal protein on growth performance,protein digestibility and trypsin gene expression in Pacific white shrimp (Litopenaeus vannamei. Six diets with0%, 12.5%, 25%, 50%, 75% and 100% of HE replacing fishmeal protein were fed four times daily to six groups of shrimp withan average initial weight of 3.53 g/shrimp. Growth of shrimp decreased with increasing level of HE substitution. Although the12.5% HE substitution caused significantly lower final weight, weight gain, SGR, feed intake, PER and PPV in comparisonwith the control diet, FCR of this diet was not statistically different (p0.05. In-vitro and in-vivo protein digestibility of 12.5% HE substitution were significantly lower than that of the controlgroup in which the trypsin gene expression of shrimp fed 12.5% HE substituted diet was the highest.

  17. A Janus Kinase in the JAK/STAT signaling pathway from Litopenaeus vannamei is involved in antiviral immune response.

    Science.gov (United States)

    Song, Xuan; Zhang, Zijian; Wang, Sheng; Li, Haoyang; Zuo, Hongliang; Xu, Xiaopeng; Weng, Shaoping; He, Jianguo; Li, Chaozheng

    2015-06-01

    The JAK/STAT signaling pathways are conserved in evolution and mediate diversity immune responses to virus infection. In the present study, a Janus kinase (designated as LvJAK) gene was cloned and characterized from Litopenaeus vannamei. LvJAK contained the characteristic JAK homology domain (JH domain) from JH1 to JH7 and showed 19% identity (34% similarity) and 21% identity (35% similarity) to Drosophila Hopscotch protein and Human JAK2 protein, respectively. The mRNA of LvJAK was highly expressed in hepatopancreas of L. vannamei and its expression level was prominently upregulated after the stimulation of Poly (I:C) and white spot syndrome virus (WSSV) challenges. There were 10 putative STAT binding motifs in the promoter region of LvJAK, and it could be regulated by LvJAK self or (and) LvSTAT, suggesting that LvJAK is the JAK/STAT pathway target gene and could function as a positive regulator to form a positive feedback loop. In addition, the silencing of LvJAK caused higher mortality rate and virus load, suggesting that LvJAK could play an important role in defense against WSSV. This is the first report about the complete set of JAK/STAT proteins in shrimp and the results provide the evidence of the positive feedback loop mediated by JAK protein present in the JAK/STAT pathway in invertebrates. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. Heat shock 70 kDa protein cognate 5 involved in WSSV toleration of Litopenaeus vannamei.

    Science.gov (United States)

    Yuan, Kai; Yuan, Feng-Hua; He, Hong-Hui; Bi, Hai-Tao; Weng, Shao-Ping; He, Jian-Guo; Chen, Yi-Hong

    2017-07-01

    The expression levels of 97 unigenes encoding heat shock proteins of Litopenaeus vannamei was scanned, and ten of them were significantly induced by white spot syndrome virus (WSSV). Among these genes, heat shock 70 kDa protein cognate 5 (LvHSC70-5) was upregulated to the highest extent and subjected to further studies. Subcellular localization assay revealed that LvHSC70-5 was located in the mitochondria. Aside from WSSV infection, unfolded protein response activation and thermal stress could also upregulate LvHSC70-5. Results of reporter gene assay demonstrated that promoter of LvHSC70-5 was activated by L. vannamei heat shock factor protein 1, activating transcription factor 4 and thermal stress. A decrease in the expression of LvHSC70-5 could reduce the aggregation of proteins in hemocytes and the cumulative mortality of WSSV-infected L. vannamei. LvHSC70-5 in L. vannamei hemocytes was upregulated by mild thermal stress. In addition, mild thermal stress, decreased the copy number of WSSV in shrimp muscle and the cumulative mortality of WSSV-infected L. vannamei. Therefore, collecting results suggested that LvHSC70-5 should be involved in WSSV toleration of shrimp L. vannamei. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Predictive ability of genomic selection models for breeding value estimation on growth traits of Pacific white shrimp Litopenaeus vannamei

    Science.gov (United States)

    Wang, Quanchao; Yu, Yang; Li, Fuhua; Zhang, Xiaojun; Xiang, Jianhai

    2017-09-01

    Genomic selection (GS) can be used to accelerate genetic improvement by shortening the selection interval. The successful application of GS depends largely on the accuracy of the prediction of genomic estimated breeding value (GEBV). This study is a first attempt to understand the practicality of GS in Litopenaeus vannamei and aims to evaluate models for GS on growth traits. The performance of GS models in L. vannamei was evaluated in a population consisting of 205 individuals, which were genotyped for 6 359 single nucleotide polymorphism (SNP) markers by specific length amplified fragment sequencing (SLAF-seq) and phenotyped for body length and body weight. Three GS models (RR-BLUP, BayesA, and Bayesian LASSO) were used to obtain the GEBV, and their predictive ability was assessed by the reliability of the GEBV and the bias of the predicted phenotypes. The mean reliability of the GEBVs for body length and body weight predicted by the different models was 0.296 and 0.411, respectively. For each trait, the performances of the three models were very similar to each other with respect to predictability. The regression coefficients estimated by the three models were close to one, suggesting near to zero bias for the predictions. Therefore, when GS was applied in a L. vannamei population for the studied scenarios, all three models appeared practicable. Further analyses suggested that improved estimation of the genomic prediction could be realized by increasing the size of the training population as well as the density of SNPs.

  20. White Shrimp Litopenaeus vannamei That Have Received Gracilaria tenuistipitata Extract Show Early Recovery of Immune Parameters after Ammonia Stressing

    Science.gov (United States)

    Chen, Yu-Yuan; Chen, Jiann-Chu; Lin, Yong-Chin; Yeh, Su-Tuen; Huang, Chien-Lun

    2015-01-01

    White shrimp Litopenaeus vannamei immersed in seawater (35‰) containing Gracilaria tenuistipitata extract (GTE) at 0 (control), 400, and 600 mg/L for 3 h were exposed to 5 mg/L ammonia-N (ammonia as nitrogen), and immune parameters including hyaline cells (HCs), granular cells (GCs, including semi-granular cells), total hemocyte count (THC), phenoloxidase (PO) activity, respiratory bursts (RBs), superoxide dismutase (SOD) activity, lysozyme activity, and hemolymph protein level were examined 24~120 h post-stress. The immune parameters of shrimp immersed in 600 mg/L GTE returned to original values earlier, at 96~120 h post-stress, whereas in control shrimp they did not. In another experiment, shrimp were immersed in seawater containing GTE at 0 and 600 mg/L for 3 h and examined for transcript levels of immune-related genes at 24 h post-stress. Transcript levels of lipopolysaccharide and β-1,3-glucan binding protein (LGBP), peroxinectin (PX), cytMnSOD, mtMnSOD, and HSP70 were up-regulated at 24 h post-stress in GTE receiving shrimp. We concluded that white shrimp immersed in seawater containing GTE exhibited a capability for maintaining homeostasis by regulating cellular and humoral immunity against ammonia stress as evidenced by up-regulated gene expression and earlier recovery of immune parameters. PMID:26058012

  1. TAT improves in vitro transportation of fortilin through midgut and into hemocytes of white shrimp Litopenaeus vannamei

    Science.gov (United States)

    Zhou, Yi; Zhang, Wenbing; Mai, Kangsen; Xu, Wei; Zhang, Yanjiao; Ai, Qinghui; Wang, Xiaojie

    2012-06-01

    Fortilin is a multifunctional protein implicated in many important cellular processes. Since injection of Pm-fortilin reduces shrimp mortality caused by white spot syndrome virus (WSSV), there is potential application of fortilin in shrimp culture. In the present study, in order to improve trans-membrane transportation efficiency, the protein transduction domain of the transactivator of transcription (TAT) peptide was fused to fortilin. The Pichia pastoris yeast expression system, which is widely accepted in animal feeds, was used for production of recombinant fusion protein. Green fluorescence protein (GFP) was selected as a reporter because of its intrinsic visible fluorescence. The fortilin, TAT and GFP fusion protein were constructed. Their trans-membrane transportation efficiency and effects on immune response of shrimp were analyzed in vitro. Results showed that TAT peptide improved in vitro uptake of fortilin into the hemocytes and midgut of Litopenaeus vannamei. The phenoloxidase (PO) activity of hemocytes incubated with GFP-Fortilin or GFP-Fortilin-TAT was significantly increased compared with that in the control without expressed fortilin. The PO activity of hemocytes incubated with 200 μg mL-1 GFP-Fortilin-TAT was significantly higher than that in the group with the same concentration of GFP-Fortilin. Hemocytes incubated with GFP-Fortilin-TAT at all concentrations showed significantly higher nitric oxide synthase (NOS) activity than those in the control or in the GFP-Fortilin treatment. The present in vitro study indicated that TAT fusion protein improved the immune effect of fortilin.

  2. ZResponse to selection, heritability and genetic correlations between body weight and body size in Pacific white shrimp, Litopenaeus vannamei

    Science.gov (United States)

    Andriantahina, Farafidy; Liu, Xiaolin; Huang, Hao; Xiang, Jianhai

    2012-03-01

    To quantify the response to selection, heritability and genetic correlations between weight and size of Litopenaeus vannamei, the body weight (BW), total length (TL), body length (BL), first abdominal segment depth (FASD), third abdominal segment depth (TASD), first abdominal segment width (FASW), and partial carapace length (PCL) of 5-month-old parents and of offspnng were measured by calculating seven body measunngs of offspnng produced by a nested mating design. Seventeen half-sib families and 42 full-sib families of L. vannamei were produced using artificial fertilization from 2-4 dams by each sire, and measured at around five months post-metamorphosis. The results show that hentabilities among vanous traits were high: 0.515±0.030 for body weight and 0.394±0.030 for total length. After one generation of selection. the selection response was 10.70% for offspring growth. In the 5th month, the realized heritability for weight was 0.296 for the offspnng generation. Genetic correlations between body weight and body size were highly variable. The results indicate that external morphological parameters can be applied dunng breeder selection for enhancing the growth without sacrificing animals for determining the body size and breed ability; and selective breeding can be improved significantly, simultaneously with increased production.

  3. BAC end sequencing of Pacific white shrimp Litopenaeus vannamei: a glimpse into the genome of Penaeid shrimp

    Science.gov (United States)

    Zhao, Cui; Zhang, Xiaojun; Liu, Chengzhang; Huan, Pin; Li, Fuhua; Xiang, Jianhai; Huang, Chao

    2012-05-01

    Little is known about the genome of Pacific white shrimp ( Litopenaeus vannamei). To address this, we conducted BAC (bacterial artificial chromosome) end sequencing of L. vannamei. We selected and sequenced 7 812 BAC clones from the BAC library LvHE from the two ends of the inserts by Sanger sequencing. After trimming and quality filtering, 11 279 BAC end sequences (BESs) including 4 609 pairedends BESs were obtained. The total length of the BESs was 4 340 753 bp, representing 0.18% of the L. vannamei haploid genome. The lengths of the BESs ranged from 100 bp to 660 bp with an average length of 385 bp. Analysis of the BESs indicated that the L. vannamei genome is AT-rich and that the primary repeats patterns were simple sequence repeats (SSRs) and low complexity sequences. Dinucleotide and hexanucleotide repeats were the most common SSR types in the BESs. The most abundant transposable element was gypsy, which may contribute to the generation of the large genome size of L. vannamei. We successfully annotated 4 519 BESs by BLAST searching, including genes involved in immunity and sex determination. Our results provide an important resource for functional gene studies, map construction and integration, and complete genome assembly for this species.

  4. Effect of salinity on the biosynthesis of amines in Litopenaeus vannamei and the expression of gill related ion transporter genes

    Science.gov (United States)

    Pan, Luqing; Liu, Hongyu; Zhao, Qun

    2014-06-01

    This study examined the effect of salinity on the expression of Na+/K+-ATPase (NKA) α-subunit and vacuolar-type H+-ATPase (V-ATPase) β-subunit gene in the gill of Litopenaeus vannamei. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay showed that the expression of NKA α-subunit and V-ATPase β-subunit gene was significantly influenced by salinity. It was found that the NKA activity significantly varied with salinity in time and dose dependent manner; whereas the V-ATPase activity did not. The abundance of NKA α-subunit gene transcript increased rapidly when the salinity decreased from 26b to 21, and slowly when the salinity decreased from 26 to 31 within the first 24 h. When the salinity decreased from 26 to 21, the transcription of NKA α-subunit gene in gill epithelium was higher at 12 h than that at 0 h, which was consistent with the result of immunoblotting assay of NKA α-subunit. In addition, salinity had a significant time- and dose-dependent effect on the concentration of biogenic amines in both hemolymph and gill. As compared to other parameters, the concentration of dopamine (DA) and 5-hydroxytryptamine (5-HT) varied in different patterns when the salinity decreased from 26 to 21 or increased from 26 to 31, suggesting that DA and 5-HT played different regulatory roles in osmotic adaption and modulation of shrimp when salinity varies.

  5. Identification and Function of Leucine-Rich Repeat Flightless-I-Interacting Protein 2 (LRRFIP2) in Litopenaeus vannamei

    Science.gov (United States)

    Zhang, Shuang; Yan, Hui; Li, Chao-Zheng; Chen, Yi-Hong; Yuan, Feng-hua; Chen, Yong-gui; Weng, Shao-Ping; He, Jian-Guo

    2013-01-01

    Leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2) is a myeloid differentiation factor 88-interacting protein with a positive regulatory function in toll-like receptor signaling. In this study, seven LRRFIP2 protein variants (LvLRRFIP2A-G) were identified in Litopenaeus vannamei. All the seven LvLRRFIP2 protein variants encode proteins with a DUF2051 domain. LvLRRFIP2s were upregulated in hemocytes after challenged with lipopolysaccharide, poly I:C, CpG-ODN2006, Vibrio parahaemolyticus, Staphylococcus aureus, and white spot syndrome virus (WSSV). Dual-luciferase reporter assays in Drosophila Schneider 2 cells revealed that LvLRRFIP2 activates the promoters of Drosophila and shrimp AMP genes. The knockdown of LvLRRFIP2 by RNA interference resulted in higher cumulative mortality of L. vannamei upon V. parahaemolyticus but not S. aureus and WSSV infections. The expression of L. vannamei AMP genes were reduced by dsLvLRRFIP2 interference. These results indicate that LvLRRFIP2 has an important function in antibacterials via the regulation of AMP gene expression. PMID:23468989

  6. Molecular characterization and function of the Prohibitin2 gene in Litopenaeus vannamei responses to Vibrio alginolyticus.

    Science.gov (United States)

    Gu, Mei-Mei; Kong, Jing-Rong; Di-Huang; Peng, Ting; Xie, Chen-Ying; Yang, Kai-Yuan; Liu, Yuan; Wang, Wei-Na

    2017-02-01

    Prohibitin2 (PHB2), a potential tumor suppressor protein, plays important roles in inhibition of cell cycle progression, transcriptional regulation, apoptosis and the mitochondrial respiratory chain. To explore its potential roles in crustaceans' immune responses we have identified and characterized LvPHB2, a 891 bp gene encoding a 297 amino acids protein in the shrimp Litopenaeus vannamei. Expression analyses showed that LvPHB2 is expressed in all examined tissues, and largely present in cytoplasm, correlating with its known anti-oxidation function in mitochondria. Luciferase reporter assays showed that over-expression of LvPHB2 could activate the p53 pathway, indicating that it might participate in apoptosis regulation. Quantitative real-time PCR revealed that infection with Vibrio alginolyticus induces its up-regulation in hepatopancreas. Moreover, RNAi knock-down of LvPHB2 in vivo raises mortality rates of L. vannamei infected by V. alginolyticus, and affects expression of STAT3, Caspase3 and p53 genes. We found significantly higher reactive oxygen species production, DNA damage and apoptosis rates in LvPHB2-silenced shrimp challenged with V. alginolyticus than in controls injected with a Green Fluorescent Protein-silencing construct. Our results suggest that LvPHB2 plays a vital role in shrimp responses to V. alginolyticus infection through its participation in regulation of oxidants and apoptosis. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. Effects of rapid temperature changes on HK, PK and HSP70 of Litopenaeus vannamei in different seasons

    Science.gov (United States)

    Guo, Biao; Wang, Fang; Dong, Shuanglin; Hou, Chunqiang

    2010-09-01

    Activities of hexokinase (HK), pyruvate kinase (PK) and levels of HSP70 were measured to evaluate the response of Litopenaeus vannamei to rapid temperature changes under controlled laboratory conditions. Shrimps were subjected to a quick temperature change from 27°C to 17°C for the summer case (Cold temperature treatment), or from 17°C to 27°C for the winter case (Warm temperature treatment). After 0.5, 1, 3, 6, 12, 24, 48, and 72 h of exposure time, shrimps were sampled and prepared for further analysis. The results showed that the effect of acute temperature changes on activities of HK was significant. Patterns of variations of the two glycolytic enzymes suggested that enzymes in the glycolysis cycle could adjust their activities to meet the acute temperature change. The HSP70 level increased in both cold and warm temperature treatments, suggesting that the rapid temperature changes activated the process of body’s self-protection. But the difference in expression peak of HSP70 might be related to the different body size and the higher thermal sensitivity to temperature increase than to temperature decrease of L. vannamei.

  8. Comparative Transcriptome Analysis in the Hepatopancreas Tissue of Pacific White Shrimp Litopenaeus vannamei Fed Different Lipid Sources at Low Salinity

    Science.gov (United States)

    Chen, Ke; Li, Erchao; Xu, Zhixin; Li, Tongyu; Xu, Chang; Qin, Jian G.; Chen, Liqiao

    2015-01-01

    RNA-seq was used to compare the transcriptomic response of hepatopancreas in juvenile Litopenaeus vannamei fed three diets with different lipid sources, including beef tallow (BT), fish oil (FO), and an equal combination of soybean oil + BT + linseed oil (SBL) for 8 weeks at 3 practical salinity unit (psu). A total of 9622 isogenes were annotated in 316 KEGG pathways and 39, 42 and 32 pathways significantly changed in the paired comparisons of FO vs SBL, BT vs SBL, or FO vs BT, respectively. The pathways of glycerolipid metabolism, linoleic acid metabolism, arachidonic acid metabolism, glycerophospholipid metabolism, fatty acid biosynthesis, fatty acid elongation, fatty acid degradation, and biosynthesis of unsaturated fatty acid were significantly changed in all paired comparisons between dietary lipid sources, and the pathways of glycerolipid metabolism, linoleic acid metabolism, arachidonic acid metabolism and glycerophospholipid metabolism significantly changed in the FO vs SBL and BT vs SBL comparisons. These pathways are associated with energy metabolism and cell membrane structure. The results indicate that lipids sources affect the adaptation of L. vannamei to low salinity by providing extra energy or specific fatty acids to change gill membrane structure and control iron balance. The results of this study lay a foundation for further understanding lipid or fatty acid metabolism in L. vannamei at low salinity. PMID:26670122

  9. Effects of periodical salinity fluctuation on the growth, molting, energy homeostasis and molting-related gene expression of Litopenaeus vannamei

    Science.gov (United States)

    Zhang, Dan; Guo, Xiantao; Wang, Fang; Dong, Shuanglin

    2016-10-01

    To determine the response of Litopenaeus vannamei to periodical salinity fluctuation, a 30-day experiment was conducted in laboratory. In this experiment, two salinity fluctuation amplitudes of 4 (group S4) and 10 (group S10) were designed. The constant salinity of 30 (group S0) was used as the control. Levels of shrimp growth, molting frequency (MF), cellular energy status (ATP, ADP and AMP), as well as the expression of genes encoding molt-inhibiting hormone (MIH), crustacean hyperglycemic hormone (CHH), ecdysteroid-regulated protein (ERP), and energy-related AMP-activated protein kinase (AMPK) were determined. The results showed that periodical salinity fluctuation significantly influenced all indicators except MF which ranged from 13.3% in group S10 to15.4% in group S4. In comparison with shrimps cultured at the constant salinity of 30, those in group S4 showed a significant elevation in growth rate, food conversion efficiency, cellular energy status, ERP and MIH gene transcript abundance, and a significant reduction in CHH and AMPK transcript abundance ( P vannamei may be highly capable of tolerating salinity fluctuation. When ambient salinity fluctuated at approx. 4, the increased MF and energy stores in organisms may aid to promoting shrimp growth.

  10. Wsv023 interacted with Litopenaeus vannamei γ-tubulin complex associated proteins 2, and decreased the formation of microtubules.

    Science.gov (United States)

    Chen, Yihong; Bi, Haitao; Li, Xiaoyun; Zhang, Zezhi; Yue, Haitao; Weng, Shaoping; He, Jianguo

    2017-04-01

    A previous study found the key transcription factor of Litopenaeus vannamei PERK-eIF2α pathway cyclic AMP-dependent transcription factor 4 (LvATF4) was involved in the transcriptional regulation of white spot syndrome virus (WSSV) gene wsv023. Knocked-down expression of LvATF4 reduced the viral copy number and the cumulative mortality of WSSV-infected shrimp. These results suggested that wsv023 may be critical to WSSV infection but the precise function of wsv023 was still unknown. By using co-immunoprecipitation and pull-down assays, we show that wsv023 interacts with L. vannamei gamma complex-associated protein 2 (LvGCP2), which is the core protein of the γ-tubulin small complex. Knocked-down, the wsv023 gene significantly reduced the copy number of WSSV in L. vannamei muscle, as well as the cumulative mortality of infected shrimp. And PERK-eIF2α pathway inhibition also showed reduced virus copy number and abrogated shrimp mortality. Furthermore, overexpression of wsv023 inhibited the formation of microtubules in 293T cells. Flow cytometry revealed that WSSV infection similarly decreased the formation of microtubules in L. vannamei haemocytes. These findings suggested that wsv023 plays a role in microtubule organization in host cells, which in turn may be beneficial to WSSV.

  11. Survival of Litopenaeus vannamei shrimp fed on diets supplemented with Dunaliella sp. is improved after challenges by Vibrio parahaemolyticus.

    Science.gov (United States)

    Medina Félix, Diana; López Elías, José Antonio; Campa Córdova, Ángel Isidro; Martínez Córdova, Luis Rafael; Luna González, Antonio; Cortes Jacinto, Edilmar; Huerta Aldaz, Nolberta; Cano Mendoza, Fernando; Burboa Zazueta, María Guadalupe

    2017-09-01

    Survival of Litopenaeus vannamei was evaluated during a Vibrio parahaemolyticus infection. This bacteria has been causing significant economic losses in the shrimp industry due to the appearance of early mortality syndrome (EMS), also known as acute hepatopancreatic necrosis disease (AHPND). Control of bacteria in ponds is difficult to achieve with antibiotics due to environmental infections and antibiotic resistance. New methods have been proposed to control and prevent the impact of bacterial infections. The physiological response indicated by plasma biochemical parameters in shrimp can determine their health and stress status. Meanwhile, shrimp immunology is the key factor in establishing strategies to control diseases. Immunostimulants are the best alternative to antibiotics to prevent or minimize disease damage, and at the same time, these stimulants improve the immune system in shrimp. Four diets containing 1.5, 2, 2.5 and 3% of Dunaliella sp. with high β-carotene content were tested in the present study. After 20days of feeding, organisms were infected with V. parahaemolyticus. Protein, glucose, lactate, triglyceride and cholesterol levels, as well as activity of prophenoloxidase and phenoloxidase, were determined 48 h post-infection (hpi). Shrimp fed a diet with 3% Dunaliella sp. showed the highest survival. Glucose, cholesterol, and triglyceride levels, as well as prophenoloxidase and phenoloxidase activity, were not observed to be suitable indicators during this bacterial infection. The results indicated that the inclusion of Dunaliella sp. in diet increases survival in L. vannamei infected with V. parahaemolyticus. Copyright © 2017. Published by Elsevier Inc.

  12. Effects of artificial infection of Litopenaeus vannamei by Micrococcus lysodeikticus and WSSV on the activity of immunity related enzymes.

    Science.gov (United States)

    Sun, Cheng-Bo; Wang, Gang; Chan, Siuming F

    2015-10-01

    In this study, the activities of 5 immunity related enzymes namely acid phosphatase (ACP), alkaline phosphatase (AKP), phenoloxidase (PO), peroxidase (POD) and lysozyme phosphatase (LZM)) of Litopenaeus vannamei after they have been injected with different concentrations of Micrococcus lysodeikticus and the white spot syndrome virus (WSSV) were examined. The cumulative mortality at 0, 24, 48, 72, 96 h was obtained. Copy numbers of WSSV in L. vannamei after a single infection, secondary infection and concurrent infection were measured. Hemolymph samples of M. lysodeikticus and WSSV injected shrimp were collected at 0, 6, 12 24, 48, 72, 78, 84, 96 and 120 h. The results were: (i) The cumulative mortality of L. vannamei increased as the shrimp were infected with higher concentration of the bacteria; (ii) The most sensitive changes of ACP, AKP and LZM were in the 6.2 × 10(5), 6.2 × 10(6), 6.2 × 10(7) cfu/mL M. lysodeikticus group; (iii) ACP but LZM were more sensitive to M. lysodeikticus than WSSV, and AKP, PO and POD is more sensitive to WSSV; (iv) The copies of WSSV in the co-injected group were higher than WSSV-single infection and WSSV-bacteria-secondary infection group at 48 h. The amount of WSSV in L. vannamei of concurrent infection and WSSV-bacteria-secondary infection groups were higher than that of the WSSV-single infection group. Copyright © 2015. Published by Elsevier Ltd.

  13. Optimal content and ratio of lysine to arginine in the diet of Pacific white shrimp, Litopenaeus vannamei

    Science.gov (United States)

    Feng, Zhengfu; Dong, Chaohua; Wang, Linlin; Hu, Yanjiang; Zhu, Wei

    2013-07-01

    The optimal quantity of dietary lysine (Lys) and arginine (Arg), and the optimal ratio of dietary Lys to Arg for Pacific white shrimp Litopenaeus vannamei were investigated. Coated Lys and Arg were added to a basal diet (37.99% crude protein and 7.28% crude lipid) to provide graded levels of Lys and Arg. The experimental diets contained three Lys levels (2.51%, 2.11%, and 1.70% of total diet), and three Arg levels (1.41%, 1.80%, and 2.21% of total diet) and all combinations of these levels were tested. Pacific white shrimp, with a mean weight of 3.62±0.1 g, were randomly distributed in 36 fiberglass tanks with 30 shrimp per tank and reared on the experimental diets for 50 days. After the feeding trial, the growth performance, survival, feed conversion rate (FCR), body composition and protease and lipase activities in the hepatopancreases of the experimental shrimps were determined. The results show that weight gain (WG), specific growth rate (SGR), FCR, body protein, body Lys and Arg content were significantly affected by dietary Lys and Arg ( P 0.05). Therefore, according to our results, the optimal Lys and Arg quantities in the diet of Pacific white shrimp, L. vannamei, were considered to be 2.11%-2.51% and 1.80%-2.21%, respectively, and the optimal ratio to be 1:0.88-1:1.05.

  14. Proteomic identification of the related immune-enhancing proteins in shrimp Litopenaeus vannamei stimulated with vitamin C and Chinese herbs.

    Science.gov (United States)

    Qiao, Jie; Du, Zhiheng; Zhang, Yueling; Du, Hong; Guo, Lingling; Zhong, Mingqi; Cao, Jingsong; Wang, Xiuying

    2011-12-01

    Recently, strong interest has been focused on immunostimulants to reducing the diseases in shrimp aquaculture. However, information regarding to the related immune-enhancing proteins in shrimps is not available yet. In this study, vitamin C (Vc), Chinese herbs (CH), and the mixture of vitamin C and Chinese herbs (Mix) were tested for their enhancement on shrimp's immune activity. Compared with those in the control group, values of phenoloxidase (PO), superoxide dismutase (SOD) and antibacterial (Ua) activity in the Mix-treated group were improved significantly 12 or 24 days after the treatment. The cumulative mortality was also lower in the Mix-treated group after infection with Vibrio parahemolyticus. Furthermore, comparative proteomic approach was used to assess the protein expression profile in shrimps. Approximately 220-290 and 300-400 protein spots were observed in the 2-DE gels. Among them, 29 and 28 altered proteins from hemocytes and hepatopancreas, respectively, were subjected to matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/MS) analysis. The results revealed that the main altered proteins showed high homologies with Litopenaeus vannamei hemocyanin, hemolymph clottable protein, hemoglobin beta, cytosolic MnSOD, trypsin, cathepsin I(L) and zinc proteinase Mpc1. Together, these studies found Vc and CH were suitable immunostimulants to shrimp L. vannamei, and 7 altered proteins could be involved in the enhanced immune activities. Copyright © 2011 Elsevier Ltd. All rights reserved.

  15. Identification of Bacterial Community Composition in Freshwater Aquaculture System Farming of Litopenaeus vannamei Reveals Distinct Temperature-Driven Patterns

    Directory of Open Access Journals (Sweden)

    Yuyi Tang

    2014-08-01

    Full Text Available Change in temperature is often a major environmental factor in triggering waterborne disease outbreaks. Previous research has revealed temporal and spatial patterns of bacterial population in several aquatic ecosystems. To date, very little information is available on aquaculture environment. Here, we assessed environmental temperature effects on bacterial community composition in freshwater aquaculture system farming of Litopenaeus vannamei (FASFL. Water samples were collected over a one-year period, and aquatic bacteria were characterized by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE and 16S rDNA pyrosequencing. Resulting DGGE fingerprints revealed a specific and dynamic bacterial population structure with considerable variation over the seasonal change, suggesting that environmental temperature was a key driver of bacterial population in the FASFL. Pyrosequencing data further demonstrated substantial difference in bacterial community composition between the water at higher (WHT and at lower (WLT temperatures in the FASFL. Actinobacteria, Proteobacteria and Bacteroidetes were the highest abundant phyla in the FASFL, however, a large number of unclassified bacteria contributed the most to the observed variation in phylogenetic diversity. The WHT harbored remarkably higher diversity and richness in bacterial composition at genus and species levels when compared to the WLT. Some potential pathogenenic species were identified in both WHT and WLT, providing data in support of aquatic animal health management in the aquaculture industry.

  16. White Shrimp Litopenaeus vannamei That Have Received Gracilaria tenuistipitata Extract Show Early Recovery of Immune Parameters after Ammonia Stressing

    Directory of Open Access Journals (Sweden)

    Yu-Yuan Chen

    2015-06-01

    Full Text Available White shrimp Litopenaeus vannamei immersed in seawater (35‰ containing Gracilaria tenuistipitata extract (GTE at 0 (control, 400, and 600 mg/L for 3 h were exposed to 5 mg/L ammonia-N (ammonia as nitrogen, and immune parameters including hyaline cells (HCs, granular cells (GCs, including semi-granular cells, total hemocyte count (THC, phenoloxidase (PO activity, respiratory bursts (RBs, superoxide dismutase (SOD activity, lysozyme activity, and hemolymph protein level were examined 24~120 h post-stress. The immune parameters of shrimp immersed in 600 mg/L GTE returned to original values earlier, at 96~120 h post-stress, whereas in control shrimp they did not. In another experiment, shrimp were immersed in seawater containing GTE at 0 and 600 mg/L for 3 h and examined for transcript levels of immune-related genes at 24 h post-stress. Transcript levels of lipopolysaccharide and β-1,3-glucan binding protein (LGBP, peroxinectin (PX, cytMnSOD, mtMnSOD, and HSP70 were up-regulated at 24 h post-stress in GTE receiving shrimp. We concluded that white shrimp immersed in seawater containing GTE exhibited a capability for maintaining homeostasis by regulating cellular and humoral immunity against ammonia stress as evidenced by up-regulated gene expression and earlier recovery of immune parameters.

  17. Application of wet waste from shrimp ( Litopenaeus vannamei) with or without sea mud to feeding sea cucumber ( Stichopus monotuberculatus)

    Science.gov (United States)

    Chen, Yanfeng; Hu, Chaoqun; Ren, Chunhua

    2015-02-01

    In the present study, the applicability of the wet waste collected from shrimp ( Litopenaeus vannamei) to the culture of sea cucumber ( Stichopus monotuberculatus) was determined. The effects of dietary wet shrimp waste on the survival, specific growth rate (SGR), fecal production rate (FPR), ammonia- and nitrite-nitrogen productions of sea cucumber were studied. The total organic matter (TOM) level in the feces of sea cucumber was compared with that in corresponding feeds. Diet C (50% wet shrimp waste and 50% sea mud mash) made sea cucumber grow faster than other diets. Sea cucumber fed with either diet D (25% wet shrimp waste and 75% sea mud mash) or sole sea mud exhibited negative growth. The average lowest total FPR of sea cucumber occurred in diet A (wet shrimp waste), and there was no significant difference in total FPR between diet C and diet E (sea mud mash) ( P > 0.05). The average ammonia-nitrogen production of sea cucumber in different diet treatments decreased gradually with the decrease of crude protein content in different diets. The average highest nitrite-nitrogen production occurred in diet E treatment, and there was no significant difference in nitrite-nitrogen production among diet A, diet B (75% wet shrimp waste and 25% sea mud mash) and diet C treatments ( P > 0.05). In each diet treatment, the total organic matter (TOM) level in feces decreased to different extent compared with that in corresponding feeds.

  18. Effect of shrimp ( Litopenaeus vannamei) farming waste on the growth, digestion, ammonium-nitrogen excretion of sea cucumber ( Stichopus monotuberculatus)

    Science.gov (United States)

    Chen, Yanfeng; Luo, Peng; Hu, Chaoqun; Ren, Chunhua

    2015-06-01

    In this study, specific growth rate (SGR), ingestion rate (IR), food conversion ratio (FCR), apparent digestion ratio (ADR) and ammonium-nitrogen excretion were determined for sea cucumber ( Stichopus monotuberculatus) reared in plastic containers (70 L; 4 containers each diet treatment). Sea cucumbers were fed with five diets containing different amounts of farming waste from shrimp ( Litopenaeus vannamei) (100%, 75%, 50%, 25% and 0) and a formulated compound (20% sea mud and 80% powdered algae). Sea cucumbers grew faster when they were fed with diet D (25% shrimp waste and 75% formulated compound) than those fed with other diets. Although IR value of sea cucumber fed with diet A (shrimp waste) was higher than those fed with other diets, both the lowest SGR and the highest FCR occurred in this diet group. The highest and the lowest ADR occurred in diet E (formulated compound) and diet A group, respectively, and the same to ammonium-nitrogen excretion. The contents of crude protein, crude lipid and total organic matter (TOM) in feces decreased in comparison with corresponding diets. In the feces from different diet treatments, the contents of crude protein and TOM increased gradually as the contents of crude protein and TOM in diets increased, while crude lipid content decreased gradually as the crude lipid content in diets increased.

  19. Evaluation of Pacific white shrimp (Litopenaeus vannamei health during a superintensive aquaculture growout using NMR-based metabolomics.

    Directory of Open Access Journals (Sweden)

    Tracey B Schock

    Full Text Available Success of the shrimp aquaculture industry requires technological advances that increase production and environmental sustainability. Indoor, superintensive, aquaculture systems are being developed that permit year-round production of farmed shrimp at high densities. These systems are intended to overcome problems of disease susceptibility and of water quality issues from waste products, by operating as essentially closed systems that promote beneficial microbial communities (biofloc. The resulting biofloc can assimilate and detoxify wastes, may provide nutrition for the farmed organisms resulting in improved growth, and may aid in reducing disease initiated from external sources. Nuclear magnetic resonance (NMR-based metabolomic techniques were used to assess shrimp health during a full growout cycle from the nursery phase through harvest in a minimal-exchange, superintensive, biofloc system. Aberrant shrimp metabolomes were detected from a spike in total ammonia nitrogen in the nursery, from a reduced feeding period that was a consequence of surface scum build-up in the raceway, and from the stocking transition from the nursery to the growout raceway. The biochemical changes in the shrimp that were induced by the stressors were essential for survival and included nitrogen detoxification and energy conservation mechanisms. Inosine and trehalose may be general biomarkers of stress in Litopenaeus vannamei. This study demonstrates one aspect of the practicality of using NMR-based metabolomics to enhance the aquaculture industry by providing physiological insight into common environmental stresses that may limit growth or better explain reduced survival and production.

  20. Bacterial Community Associated with Healthy and Diseased Pacific White Shrimp (Litopenaeus vannamei Larvae and Rearing Water across Different Growth Stages

    Directory of Open Access Journals (Sweden)

    Yanfen Zheng

    2017-07-01

    Full Text Available Bacterial communities are called another “organ” for aquatic animals and their important influence on the health of host has drawn increasing attention. Thus, it is important to study the relationships between aquatic animals and bacterial communities. Here, bacterial communities associated with Litopenaeus vannamei larvae at different healthy statuses (diseased and healthy and growth stages (i.e., zoea, mysis, and early postlarvae periods were examined using 454-pyrosequencing of the 16S rRNA gene. Bacterial communities with significant difference were observed between healthy and diseased rearing water, and several bacterial groups, such as genera Nautella and Kordiimonas could also distinguish healthy and diseased shrimp. Rhodobacteraceae was widely distributed in rearing water at all growth stages but there were several stage-specific groups, indicating that bacterial members in rearing water assembled into distinct communities throughout the larval development. However, Gammaproteobacteria, mainly family Enterobacteriaceae, was the most abundant group (accounting for more than 85% in shrimp larvae at all growth stages. This study compared bacterial communities associated with healthy and diseased L. vannamei larvae and rearing water, and identified several health- and growth stage-specific bacterial groups, which might be provided as indicators for monitoring the healthy status of shrimp larvae in hatchery.

  1. Molecular modeling and expression of the Litopenaeus vannamei proliferating cell nuclear antigen (PCNA) after white spot syndrome virus shrimp infection.

    Science.gov (United States)

    de-la-Re-Vega, Enrique; Muhlia-Almazan, Adriana; Arvizu-Flores, Aldo A; Islas-Osuna, Maria A; Yepiz-Plascencia, Gloria; Brieba, Luis G; Sotelo-Mundo, Rogerio R

    2011-01-01

    Proliferating cell nuclear antigen (PCNA) is the eukaryotic sliding clamp that tethers DNA polymerase to DNA during replication. The full-length cDNA of the Pacific white shrimp Litopenaeus vannamei PCNA (LvPCNA) was cloned and encoded a protein of 260 amino acids that is highly similar to other Crustacean PCNAs. The theoretical shrimp PCNA structure has all the domains that are necessary for its interaction with template DNA and DNA polymerase. RT-PCR analysis showed that LvPCNA is expressed mainly in muscle and hemocytes and much less in hepatopancreas and gills. LvPCNA mRNA levels are not statistically different in muscle from healthy and challenged shrimp with the white spot syndrome virus (WSSV). In contrast, the mRNA levels of the viral DNA polymerase show a biphasic pattern with expression at 6 h post-infection and later at 24 and 48 h. These results suggest that in shrimp muscle LvPCNA levels are steadily kept to allow viral replication and that WSSV DNA polymerase (WSSV-DNApol) is more responsive towards later stages of infection. More knowledge of the DNA replication machinery would result in a better understanding of the mechanism and components of viral replication, since the WSSV genome does not have all the components required for assembly of a fully functional replisome.

  2. Dextrose as carbon source in the culture of Litopenaeus vannamei (Boone, 1931 in a zero exchange system

    Directory of Open Access Journals (Sweden)

    Sabrina M Suita

    2015-07-01

    Full Text Available This work compared the use of dextrose and molasses as carbon sources for biofloc development, water quality maintenance, microorganism composition and growth performance of Litopenaeus vannamei juveniles in biofloc technology (BFT. Two treatments, dextrose and molasses, were tested with four replicates each. Carbon was added to achieve a C:N-AT (N-(NH3+NH4+ ratio of 6:1. Physical and chemical water quality variables were monitored daily, and shrimp growth was estimated through periodic biometry. After 30 days, survival, final biomass, and feeding conversion rate (FCR were determined. Dissolved organic carbon, chlorophyll-a, floc volume, total ammonia, nitrite, nitrate and phosphate concentrations, and microorganisms (qualified by groups, were measured every three days. Water quality variables remained within acceptable levels throughout the experimental period, except for nitrite, which reached higher levels than recommended for this species. The use of dextrose resulted in higher water transparency, which influenced the remaining centric diatoms. A superior shrimp performance was observed at this treatment, presumably because of variations on the microbial community. Therefore, it is concluded that the addition of dextrose results in a superior growth performance of L. vannamei when cultured in BFT systems.

  3. Viabilidad poblacional de Alouatta palliata (Primates: Atelidae y Cebus capucinus (Primates: Cebidae en el Refugio de Vida Silvestre Privado Nogal, Sarapiquí, Heredia, Costa Rica Population viability of Alouatta palliata (Primates: Atelidae and Cebus capucinus (Primates: Cebidae at Refugio de Vida Silvestre Privado Nogal, Sarapiquí, Heredia, Costa Rica

    Directory of Open Access Journals (Sweden)

    Jorge Rodríguez-Matamoros

    2012-06-01

    Full Text Available La destrucción del hábitat conlleva a la fragmentación de poblaciones de especies silvestres y se considera como uno de los principales factores en la extinción de especies A medida que las poblaciones se vuelven más pequeñas, surgen amenazas hacia su estabilidad y persistencia, como resultado de factores estocásticos demográficos, ambientales y genéticos. El objetivo de este trabajo fue estudiar los efectos de la fragmentación de poblaciones en la viabilidad de Alouatta palliata y Cebus capucinus en el Refugio de Vida Silvestre Privado Nogal (RVSPN, Sarapiquí, Heredia. Para esto se uso el programa VORTEX para correr un análisis de viabilidad de poblaciones (PVA para ambas especies. La información utilizada en el PVA proviene de la estructura demográafica de las poblaciones del RVSPN, literatura sobre la historia natural de las especies y artículos relacionados con PVA. Los resultados sugieren que tanto A. palliata como C. capucinus pueden sobrevivir en fragmentos boscosos aislados. Sin embargo, si se incorporan factores como depresión por endogamia, catástrofes o perdida de hábitat, las pequeñas poblaciones fragmentadas se vuelven inestables y aumenta el riesgo de que desaparezcan. Las poblaciones continuas fueron más robustas ante las amenazas incorporadas, por lo que se recomienda continuar con la reforestación para unir los fragmentos boscosos. Es importante darle seguimiento a las poblaciones de ambas especies y tener un manejo de su hábitat para disminuir los efectos negativos de diferentes eventos estocásticos provocados por el ambiente.Population viability of Alouatta palliata (Primates: Atelidae and Cebus capucinus (Primates: Cebidae at Refugio de Vida Silvestre Privado Nogal, Sarapiquí, Heredia, Costa Rica. Habitat destruction may cause wildlife population fragmentation and is considered an important factor in small population species extinction. As wildlife populations become smaller, threats to their stability

  4. Compensatory growth and production of white shrimp, Litopenaeus vannamei, in Costa Rica, during the maternity, development and grow-out commercial phases

    OpenAIRE

    Valverde-Moya, José A.; Alfaro-Montoya, Jorge

    2015-01-01

    Compensatory growth and production of Litopenaeus vannamei in commercial cultures in farms in the Gulf of Nicoya, Costa Rica, during 2014 were evaluated in this study. Growth and production were compared between three culture systems starting from an initial maternity (MA) phase with high stocking density (85/m2) of post larvae: a) maternity-development (MA-DE) with a medium stocking density (23.3/m2) and an initial weight of 1.4 g, b) maternity-development-grow-out (MA-DE-G) with a low densi...

  5. Caracterização quali-quantitativa dos efluentes gerados no cultuvo intensivo de Litopenaeus vannamei em sistema de bioflocos

    OpenAIRE

    Arantes, Rafael da Fonseca

    2014-01-01

    Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Aquicultura, Florianópolis, 2014. Determinou-se o efeito de algumas estratégias de manejo do sistema de cultivo de Litopenaeus vannamei com bioflocos sobre o uso da água e o potencial poluidor gerado pelo cultivo. Para isto foram realizados dois experimentos: o primeiro objetivou estudar o efeito de dois métodos de controle de sólidos suspensos totais sobre a qualidade e con...

  6. Tratamiento de efluentes del cultivo de Litopenaeus vannamei mediante procesos de sedimentación, filtración y absorción

    OpenAIRE

    Ramos, Roberto; Vinatea,Luis; Santos, Julia; Da Costa,Rejane

    2010-01-01

    La eficiencia de remoción de materia particulada y nutrientes disueltos de efluentes de estanques de reproductores de Litopenaeus vannamei fue evaluada a escala de laboratorio, empleando tratamientos de sedimentación, filtración por Crassostrea rhizophorae y absorción por Ulva fasciata. En cada tratamiento se empleó un tiempo de residencia hidráulica del efluente de seis horas. En la etapa de sedimentación se utilizaron estanques con 90 L del efluente. Para la etapa de filtración, se emplearo...

  7. Tratamiento de efluentes del cultivo de Litopenaeus vannamei por sedimentación y filtración por la ostra Crassostrea rhizophorae

    OpenAIRE

    Ramos, Roberto; Vinatea,Luis; Costa,Rejane da

    2008-01-01

    A escala de laboratorio se evaluaron las eficiencias de remoción del material particulado y nutrientes disueltos desde efluentes de cultivo de Litopenaeus vannamei, utilizando un sistema combinado de dos fases, sedimentación y filtración por la ostra Crassostrea rhizophorae, empleando tres tiempos de retención hidráulica (TRH) del efluente, 6, 12 y 24 h. En la fase de sedimentación, se emplearon estanques de 100 L en los cuales fueron colocados 90 L de efluente. En la fase de filtración, se u...

  8. Evaluación in vivo del efecto cicatrizante de un gel a base de quitosano obtenido de exoesqueleto de camarón blanco Litopenaeus vannamei

    OpenAIRE

    Heimy Franceline Martínez Sánchez; Amada Yerén Escobedo Lozano; Evaristo Méndez-Gómez; Alfredo Emmanuel Vázquez; Manuel de Jesús Sol Hernández; Anahí Elizabeth Osuna Lizárraga

    2014-01-01

    Healing effect of a chitosan-based gel obtained from the exoskeleton of white shrimp Litopenaeus vannamei and its evaluation in vivoTítulo corto: Evaluación del quitosano como cicatrizanteResumen:  En México alrededor del 62 % de la población sufre de accidentes causantes de alteraciones en la piel como quemaduras, heridas y diabetes principalmente. Para atender esta problemática, se propone el uso de un gel de quitosano, obtenido del exoesqueleto de camarón ya que presenta actividad antimicr...

  9. Caracterización de la textura sensorial e instrumental del camarón de cultivo Litopenaeus vannamei en la camaronera de Tunas de Zaza.

    OpenAIRE

    Tamarit Pino, Y.

    2008-01-01

    El camarón, como el resto de los alimentos, puede hacer uso de las técnicas de Evaluación Sensorial para evaluar su calidad. Dentro de los atributos sensoriales que se analizan, la textura es la primera que se afecta. El objetivo del presente trabajo consistió en constituir una Comisión de Evaluación Sensorial (CES) especializada en textura del camarón de cultivo cocido Litopenaeus vannamei, para lo cual fue necesario estudiar 12 tratamientos para variar la textura del camar...

  10. Bactérias entéricas presentes em amostras de água e camarão marinho Litopenaeus vannamei oriundos de fazendas de cultivo no Estado do Ceará, Brasil

    National Research Council Canada - National Science Library

    Parente, Lucélia Sabóia; Costa, Renata Albuquerque; Vieira, Gustavo Hitzschky Fernandes; Reis, Eliane Moura Falavina dos; Hofer, Ernesto; Fonteles, Antonio Adauto; Vieira, Regine Helena Silva dos Fernandes

    2011-01-01

    ... e identificação de coliformes, e pesquisa de Salmonella em 28 amostras de água e 28 de camarão da espécie Litopenaeus vannamei, oriundas de duas fazendas de cultivo localizadas no Estado do Ceará, Brasil...

  11. Replacement of fish meal with soybean meal, alone or in combination with distiller’s dried grains with solubles in practical diets for Pacific white shrimp, Litopenaeus vannamei, grown in a clear-water system

    Science.gov (United States)

    The objective of this study was to evaluate inclusion of distiller’s dried grains with solubles (DDGS) as partial replacement of commercial, solvent-extracted soybean meal (SBM) in fish meal-free diets for Pacific white shrimp, Litopenaeus vannamei. Aquaria connected to a recirculating biofiltratio...

  12. Endoparasitos (Nemathelminthes y Platyhelminthes de animales de vida silvestre de la Reserva de Biósfera del Manu, Perú

    Directory of Open Access Journals (Sweden)

    Manuel Tantaleán

    2013-06-01

    Full Text Available Durante el año 2001, se recolectaron 8 géneros de helmintos de mamíferos y aves silvestres del Perú, en la Reserva Biosfera del Manu (Departamentos de Cusco y Madre de Dios, desde los 470 a 3780 m. Los parásitos fueron fijados con alcohol etílico en el campo y transportados al laboratorio para estudiarlos; los nemátodos se diafanizaron y los céstodos y tremátodos se colorearon con carmín de acuerdo a técnicas convencionales. La identificación específica de algunos helmintos fue imposible por el estado en que se encontraban, pero se dan a conocer por ser material de gran importancia y ser algunos de ellos algunos registros nuevos para el Perú. Nuestros hallazgos son los siguientes: Rhopalias caballeroi Kifune y Uyema, 1982, Rhopalias sp. (probable n. sp., Taenia pisiformis Bloch, 1780 (cisticerco, Mesocestoides sp. (tetratyridia, Aprocta sp., Evaginuris branicki Mc Clure, 1932, Haematospiculum sp., Protospirura sp., Diplotriaena sp., Thelazia sp. 1, Thelazia sp. 2, Thelazia sp. 3 y Subulura sp.

  13. Desenterrado os mortos: A reconstrução do passado político brasileiro na obra de Edney Silvestre

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    Gabriela Antunes

    2014-07-01

    Full Text Available Este artigo tem como objetivo analisar a trilogia de estreia de Edney Silvestre, composta por Se eu fechar os olhos agora (2009, A felicidade é fácil (2011, e Vidas provisórias (2013. Pretende-se fazer um breve balanço da contribuição do autor para a reabertura de um debate sobre o recente passado histórico-político brasileiro, e, relacionando os fatos abordados no romance com temas contemporâneos, ressaltar a relevância e a atualidade desse debate. Para isso, os textos serão analisados cronologicamente, o que visa mostrar o desenvolvimento do tratamento do sujeito histórico no conjunto de obras do autor.   This paper aims to analyse Edney Silvestre’s trilogy composed by If I close my eyes now (2009, Happiness is easy (2011, and Temporary lives (2013, as well as to review the author’s contribution in reopening a debate on Brazilian’s recent historical and political past. By pointing out the relationship between the facts mentioned in thenovels and contemporary Brazil, it aims to stress the importance and up-to-dateness of this discussion. In order to achieve these goals, the novels will be analysed chronologically, which also intends to reveal the development of the way the author deals with Brazilian history throughout his oeuvre.

  14. Fecundity of Uca uruguayensis and Chasmagnathus granulatus (Decapoda, Brachyura from the "Refugio de Vida Silvestre" Bahía Samborombón, Argentina

    Directory of Open Access Journals (Sweden)

    II. César

    Full Text Available The aim of the present work conducted at the Refugio de Vida Silvestre Bahía Samborombón is to analyse the most relevant aspects of the fecundity of Chasmagnathus granulatus and Uca uruguayensis. Samplings were carried out from March 2001 to February 2003. Ovigerous females of U. uruguayensis (N = 13 and C. granulatus (N = 25 were found during spring and summer, their sizes (CW varied from 9.1 to 11.7 µm for the former species and from 22.8 to 32.4 mm for the latter. The egg diameter in U. uruguayensis ranged from 245 to 260 µm for embryos in the early stage of development and from 250 to 345 µm for those in mid-developmental stage, while in C. granulatus from 250t o 345 µm and from 260 to 365 µm respectively. Fecundity varied from 1126 to 6745 eggs/brood in U. uruguayensis and 15688-57418 eggs/brood in C. granulatus. For those females with broods in mid-developmental stage, several relationships were made. For U. uruguayensis the best correlation coefficients were obtained for the relationships: female weight vs. egg mass weight and carapace width vs. egg mass weight; for C. granulatus the best association was obtained between female size and the egg number and the egg mass weight.

  15. Híbridos somáticos obtenidos por fusión de protoplastos entre Solanum tuberosum L. subsp. tuberosum y la especie silvestre Solanum circaeifolium Bitter

    Directory of Open Access Journals (Sweden)

    Rosa Espejo

    2013-03-01

    Full Text Available Con la finalidad de obtener híbridos somáticos interespecíficos, se fusionaron protoplastos de la especie tetraploide Solanum tuberosum y de la especie silvestre diploide Solanum circaeifolium utilizando polietilenglicol. Los productos de fusión fueron cultivados en el medio V-KM suplementado con albúmina de suero bovino. Las primeras divisiones celulares ocurrieron a los 3 a 4 días de cultivo. Después de la formación de colonias se observó una rápida proliferación de callos, a partir de los cuales se regeneraron 19 plantas. El análisis molecular usando RAPD, confirmó que los regenerantes presentaban segmentos de ADN de ambos parentales, sugiriendo su posible naturaleza de híbridos somáticos. Las observaciones del número de cromosomas indicaron que todos los híbridos fueron aneuploides. En condiciones de invernadero, los regenerantes derivados de la fusión de protoplastos, mostraron características morfológicas intermedias entre las líneas parentales. Este estudio muestra la producción de híbridos somáticos de papa con el método de fusión presentado.

  16. Efecto del fotoperiodo y la temperatura sobre la composición bioquímica en reproductores silvestres de cabrilla sardinera, Mycteroperca rosacea (Streets, 1877

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    José Antonio Estrada-Godínez

    2014-03-01

    Full Text Available Se estimó el factor de condición (K, el índice gonadosomático (IGS, hepatosomático (IHS y de grasa visceral (IGV, así como la composición bioquímica en diferentes tejidos de reproductores silvestres de cabrilla sardinera, Mycteroperca rosacea, para evaluarlos a lo largo de un ciclo reproductivo y ver la relación que presentan los cambios con respecto a la temperatura del agua y fotoperiodo de la zona de muestreo. Se capturaron 187 reproductores, 146 hembras y 41 machos en el golfo de California, México. Se observaron diferencias significativas (P < 0,005 en los IGS, IHS e IGV; en el caso de K no se encontraron diferencias. También, se observaron variaciones significativas (P < 0,05 en la mayoría de los parámetros bioquímicos, encontrándose los valores más altos durante la etapa de desove, mientras que los más bajos durante la etapa de reposo. Todos los índices estimados y los parámetros bioquímicos determinados, se correlacionaron significativamente (P < 0,05 con el fotoperiodo, mientras que solo se encontraron correlaciones significativas (P < 0,05 entre el IGS e IGV con respecto a la temperatura del agua.

  17. Oxygen consumption of Litopenaeus vannamei juveniles in heterotrophic medium with zero water exchange Consumo de oxigênio de juvenis de Litopenaeus vannamei em meio heterotrófico sem renovação de água

    Directory of Open Access Journals (Sweden)

    Luis Vinatea

    2009-05-01

    Full Text Available This work aimed at determining the dissolved oxygen consumption rate of Litopenaeus vannamei juveniles maintained in a microbial biofloc raceway system at high density with no aeration. Three 4 L bottles were filled for each treatment, sealed hermetically, and placed in an enclosed greenhouse raceway system. Four shrimp (13.2±1.42 g were assigned to two sets of the bottles, which underwent the following treatments: light conditions with no shrimp; dark conditions with no shrimp; light conditions with shrimp; and dark conditions with shrimp. Dissolved oxygen content was measured every 10 min for 30 min. A quadratic behavior was observed in dissolved oxygen concentration over time. Significant differences for oxigen consumption were observed only at 10 and 20 min between shrimp maintained in the dark and those under light conditions. At 10 min, a higher value was observed in shrimp maintained under light, and at 20 min, in the dark. Significant differences between 10 and 20 min and between 10 and 30 min were observed when oxygen consumption was analyzed over time in the presence of light. Under dark conditions there were significant differences only between 20 and 30 min. Lethal oxygen concentration (0.65 mg L-1 would be reached in less than one hour either under light or dark conditions with no aeration.O objetivo deste trabalho foi determinar o consumo de oxigênio dissolvido (OD de juvenis de Litopenaeus vannamei mantidos em sistema de cultivo de bioflocs bacterianos em alta densidade e ausência de aeração. Três garrafas de 4 L foram preenchidas para cada tratamento, fechadas hermeticamente e colocadas em sistema de cultivo fechado. Quatro camarões (13,2±1,42 g foram colocados em dois dos conjuntos de garrafas. Os tratamentos aplicados foram: luminosidade, sem camarões; escuro, sem camarões; luminosidade, com camarões; escuro, com camarões. A concentração de oxigênio dissolvido foi determinada a cada 10 min durante 30 min. Foi

  18. Efeito do tipo de sedimento na eficiência alimentar, crescimento e sobrevivência de Litopenaeus vannamei (Boone, 1931 = Effect of sediment type on feed efficiency, growth and survival of Litopenaeus vannamei (Boone, 1931

    Directory of Open Access Journals (Sweden)

    Daniele Bezerra dos Santos

    2011-09-01

    Full Text Available Este estudo avaliou o desempenho zootécnico do camarão marinho Litopenaeus vannamei em diferentes tipos de substratos não consolidados. Juvenis (0,97 ± 0,27 g foram aclimatados na densidade de 52 m-2, em 21 caixas de polietileno com biofiltros individuais, emum sistema fechado de filtração contínua, sendo submetidos aos tratamentos: 1 A = 25% silte + 25% argila + 25% areia muito fina + 25% areia fina; 2 B = 50% areia fina + 50% areia muito fina e 3 Controle = sem substrato, em um delineamento experimental completamente casualizado, com três tratamentos e sete repetições cada. Os animais foram alimentados com 12% da biomassa/dia com ração peletizada (35% de proteína bruta, ofertada em bandejas e parceladaem duas ofertas diárias (8 e 16h. O estudo teve duração de 48 dias, sendo avaliados: consumo aparente de ração, eficiência alimentar, taxa de crescimento específico, sobrevivência e ganho de peso. Foram acompanhados diariamente salinidade, pH, amônia e temperatura da água. A análise estatística dos dados se deu por meio dos testes Anova ou Kuskal-Wallis, em função da sua parametricidade. Não foram observadas diferenças significativas com relação aos fatores avaliadosnas diferentes granulometrias de sedimento testadas, indicando que estas não exerceram influência sobre o desempenho zootécnico de L. vannamei juvenil.This study aimed to evaluate the zootechnical performance of Litopenaeus vannamei shrimp at different types of unconsolidated substrate. Juvenile shrimp (0.97 ± 0.27 g were acclimatized, in a stocking density of 52 m-2, to 21 polyethylene boxes (50 L connected individually to biofilters in a closed water exchange system. The animals were submitted to the treatments: 1 A = 25% silt + 25% clay + 25% very fine sand + 25% fine sand; 2 B = 50% fine sand + 50% very fine sand and 3 Control = no sediment, with seven replications each. Photoperiod was set on 12L: 12D. Shrimps were fed twice a day (at08:00 and

  19. Acute toxicity of pyrazosulfuron-ethyl and permethrin to juvenile Litopenaeus vannamei =Toxicidade aguda de pirazossulfurom-etílico e permethrin em juvenis de camarão branco Litopenaeus vannamei

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    Giovanni Lemos de Mello

    2011-01-01

    Full Text Available The objective of this study was to determine the LC50 (96h of two pesticides: SiriusR 250 SC herbicide of the pyrazosulfuron-ethyl group, and TalcordR insecticide of the permethrin group, on juvenile Litopenaeus vannamei. Shrimp total hemocyte count (THC was also determined as an indication of physiological alterations caused by the pesticides. Juvenile shrimp (5.0 } 0.5 g were exposed to the following concentrations: 0, 0.1, 1.0, 10, 100 and 1000 ƒÊg L-1 SiriusR 250 SC; and 0, 0.001, 0.01, 0.1, 1.0 and 10 ƒÊg L-1 TalcordR. The TalcordR LC50 (96h was of 0.00933 ƒÊg L-1 or 9.33 ng L-1. There were no significant changes in the THC between control and test groups. No SiriusR 250 SC concentrations tested killed more than 50%of the shrimp; therefore, the herbicide was considered not toxic to the juveniles. However, the THC showed significant differences between the control and test groups, suggesting sublethal effects to L. vannamei juveniles. According to the results, the insecticide TalcordR is highly lethal for L. vannamei and the herbicide SiriusR 250 SC was not lethal in the concentrations tested but showed sublethal effects as lower THC. The results demonstrate the risks involved in farming L. vannamei shrimp near rice cultures where these pesticides are routinely used.O objetivo deste trabalho foi determinar a CL50 (96h dos agroquimicos SiriusR 250 SC, herbicida a base de pirazossulfurom-etilico, e TalcordR, inseticida a base de permethrin, em juvenis de Litopenaeus vannamei, bem como avaliar possiveis alteracoes fisiologicas por meio da contagem total de hemocitos (CTH dos camaroes. Juvenis de L. vannamei (5,0 } 0,5 g foram expostos as seguintes concentracoes dos agroquimicos: SiriusR 250 SC, 0; 0,1; 1; 10; 100 e 1.000 ƒÊg L-1, e TalcordR, 0; 0,001; 0,01; 0,1; 1 e 10 ƒÊg L-1. A CL50 (96h do inseticida TalcordR foi de 0,00933 ƒÊg L-1 ou 9,33 ng L-1. Nao houve alteracoes significativas da CTH entre as medias dos grupos

  20. Screening three Finfish Species for their Potential in Removing Organic Matter from the Effluent of White Leg Shrimps (Litopenaeus vannamei Farming

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    Nguyen, LQ.

    2016-01-01

    Full Text Available White leg shrimp (Litopenaeus vannamei farming effluent contains pollutants that include high levels of organic matter (OM nutrients and growth-promoting substances. This study investigated the effects of varied concentrations of white leg shrimp (Litopenaeus vannamei farm wastewater 0, 50, 75 and 100%, on the survival rate (SR of three finfish species: tilapia (Oreochromis niloticus, grey mullet (Mugil cephalus and rabbit fish (Siganus guttatus as part of screening their potential in removing organic matter from the effluent of white leg shrimp farming. The different initial levels of shrimp wastewater from 50% to 100% had no significant effect on the survival rate of tilapia and mullet; but the survival rate of S. guttatus significantly decreased with increasing shrimp wastewater (P<0.05. The results showed that the removal of BOD, COD and TSS occurred in the range of 66-83, 68-81 and 30-54%; respectively and the removal efficiency of OM by mullet was higher than Tilapia in all treatments. The study also indicated that the reduction highest removal of BOD, COD and TSS was achieved being 83.1%, 80.7and 53,7% respectively, at the medium stocking density (25 fish/m2 of mullet.

  1. Species identification of white shrimps Litopenaeus vannamei and other Penaeus sp. by using PCR-RFLP of Cytochrome Oxidase Subunit I (COI gene

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    Chotigeat, W.

    2004-05-01

    Full Text Available The morphology of imported white shrimp (Litopenaeus vannamei is similar to other native white shrimp of the Thai peninsula (Penaeus merguiensis, Penaeus silasi and Penaeus indicus and could not be separated clearly in the juvenile stage. It causes the mixture of them in the culture pond leading to problems on the farm. This work identified the different pattern of cytochrome oxidase subunit I (COI gene in mitochondrial DNA (mtDNA of 7 species (Litopenaeus vannamei, Penaeus merguiensis, Penaeus indicus, Penaeus silasi, Penaeus monodon, Penaeus semisalcatus and Metapenaeus sp. using PCR-RFLP technique. The COI gene fragment was amplified by PCR method and subsequently cleaved by restriction enzyme, RsaI, providing specific bands, 282 bp and 526 bp in L. vannamei, 300 and 582 bp in P. silasi, 295 bp and 555 bp in P. monodon, 376 bp and 524 bp in P. semisulcatus, and 100 bp and 800 bp in Metapenaeus sp; four specific bands, 142 bp, 152 bp, 240 bp and 360 bp in P. merguiensis and 282 bp, 248 bp and 334 bp in P. indicus. This method can be applied to identify and clearly separate the pacific white shrimp (L. vannamei from other morphologically similar species and distant species.

  2. A comparative integrated gene-based linkage and locus ordering by linkage disequilibrium map for the Pacific white shrimp, Litopenaeus vannamei.

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    Jones, David B; Jerry, Dean R; Khatkar, Mehar S; Raadsma, Herman W; Steen, Hein van der; Prochaska, Jeffrey; Forêt, Sylvain; Zenger, Kyall R

    2017-09-04

    The Pacific whiteleg shrimp, Litopenaeus vannamei, is the most farmed aquaculture species worldwide with global production exceeding 3 million tonnes annually. Litopenaeus vannamei has been the focus of many selective breeding programs aiming to improve growth and disease resistance. However, these have been based primarily on phenotypic measurements and omit potential gains by integrating genetic selection into existing breeding programs. Such integration of genetic information has been hindered by the limited available genomic resources, background genetic parameters and knowledge on the genetic architecture of commercial traits for L. vannamei. This study describes the development of a comprehensive set of genomic gene-based resources including the identification and validation of 234,452 putative single nucleotide polymorphisms in-silico, of which 8,967 high value SNPs were incorporated into a commercially available Illumina Infinium ShrimpLD-24 v1.0 genotyping array. A framework genetic linkage map was constructed and combined with locus ordering by disequilibrium methodology to generate an integrated genetic map containing 4,817 SNPs, which spanned a total of 4552.5 cM and covered an estimated 98.12% of the genome. These gene-based genomic resources will not only be valuable for identifying regions underlying important L. vannamei traits, but also as a foundational resource in comparative and genome assembly activities.

  3. [Mass spectrometry identification and immune cross-reactivity of a minor shrimp allergen-sarcoplasmic calcium binding protein from Litopenaeus vannamei].

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    Wang, Cai-xia; Huang, Jian-fang; Xiang, Jun-jian; Sun, Yi-fan; Lv, Si; Guo, Jie

    2012-08-01

    To identify sarcoplasmic calcium-binding protein (SCP) as a minor shrimp allergen by mass spectrometry, and to analyze the immune cross-reactivity among crustacean SCPs. The M(r); 21 000 allergen from Litopenaeus vannamei was identified by MALDI-TOF/TOF-MS. BLAST and ClustalW were used to compare amino acid sequence identity of the allergen among crustaceans. The puritifed M(r); 21 000 allergen was injected subcutaneously in mice to produce the specific polyclonal antibodies to analyze immune cross-reactivity of the allergen with proteins from 8 other species of crustaceans by Western blotting. The M(r); 21 000 shrimp allergen was identified as SCP. Sequence comparison revealed that SCP had 81%-100% amino acid identity among crustaceans. Western blotting showed that the proteins with M(r); about 21 000, corresponding to SCP from Metapenaeus ensis, Penaeus monodon, Oratosquilla oratoria, Macrobrachium rosenbergii, Procambarus clarkii, Portunus pelagicus, Charybdis feriatus, Eriocheir sinensis were recognized by polyclonal antibodies against SCP of Litopenaeus vannamei. SCP is a minor shrimp allergen, and SCPs have a high sequence homology and strong immune cross-reactivity among crustaceans, which can be used as detective, diagnostic and safe immunotherapeutic agents for subjects with shrimp allergy.

  4. Characterization of a vasa-like gene from the pacific white shrimp Litopenaeus vannamei and its expression during oogenesis.

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    Aflalo, Eli D; Bakhrat, Anna; Raviv, Shaul; Harari, Daniel; Sagi, Amir; Abdu, Uri

    2007-02-01

    The vasa gene encodes an ATP-dependent RNA helicase belonging to the DEAD-box family that, in many organisms, is specifically expressed in germline cells throughout the life cycle. In this study we first cloned Pacific white shrimp (Litopenaeus vannamei) partial cDNAs of two members of the DEAD-box family, one belonging to the vasa subfamily (Lv-Vasa) and the other to the PL10 subfamily (Lv-PL10). Examination of their spatial expression pattern in adult tissues revealed that Lv-Vasa is restricted to the gonads, whereas Lv-PL10 is found in gonads as well as in somatic tissues. Next, we cloned the full-length shrimp vasa cDNA and found that Lv-Vasa encoded a protein with a DEAD-like helicase domain followed by a helicase superfamily C-terminal domain. In addition, Lv-Vasa encoded N-terminal three repeats of the C2HC-type zinc finger domain, a motif encoded by vasa genes of several crustaceans and several other invertebrate organisms. In situ hybridization of ovarian sections showed that the Lv-Vasa transcript is localized to the cytoplasm of the oocyte throughout oogenesis. The abundance of Lv-Vasa mRNA in mature oocytes suggests a maternal contribution for the developing embryo. It is demonstrated that the vasa homolog from L. vannamei is a gonad specific germline cell marker that could be exploited to enhance our understanding of developmental and reproductive processes in the germline of this economically important shrimp.

  5. A Novel Vascular Endothelial Growth Factor Receptor Participates in White Spot Syndrome Virus Infection in Litopenaeus vannamei

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    Shihao Li

    2017-11-01

    Full Text Available Vascular endothelial growth factor (VEGF signaling pathway is known to play key roles in endothelial cell proliferation, migration, angiogenesis, vascular permeability, inhibition of apoptosis, and virus infection. In the present study, a novel VEGFR gene (LvVEGFR2 was identified and characterized from Litopenaeus vannamei. The deduced amino acid sequence of LvVEGFR2 possessed typical features of VEGFRs reported in other species, including six IG-like domains, a transmembrane motif, a protein kinase (PK domain, and one tyrosine-PK active site. The transcripts of LvVEGFR2 were mainly detected in hemocytes and lymphoid organ (Oka. Subcellular localization analysis showed that LvVEGFR2 was a membrane protein. Its expression level was obviously upregulated in hemocytes and Oka of the shrimp after white spot syndrome virus (WSSV infection. Knockdown of LvVEGFR2 gene expression by double-strand RNA mediated interference could lead to a decrease of virus copy number in WSSV-infected shrimp. The interaction between LvVEGFR2 and different LvVEGFs (LvVEGF1, LvVEGF2, and LvVEGF3 in shrimp was analyzed at the transcription level and protein level, respectively. Knockdown of LvVEGF2 or LvVEGF3 could downregulate the expression level of LvVEGFR2, and injection of the recombinant LvVEGF2 or LvVEGF3 could upregulate the expression level of LvVEGFR2. Yeast two-hybrid analysis showed that LvVEGFR2 could interact with LvVEGF2 and LvVEGF3 directly. The study improved our understanding on the VEGF signaling pathway of shrimp and its role during WSSV infection.

  6. Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170

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    Irasema E Luis-Villaseñor

    2015-09-01

    Full Text Available The protective effects of two probiotic mixtures was studied using the fingerprints of the bacterial community of Litopenaeus vannamei juveniles exposed to probiotics and challenged with Vibrio parahaemolyticus CAIM 170. Fingerprints were constructed using 16S rRNA gene and the PCR-SSCP (Single strand conformation polymorphism technique, and the probiotics used were an experimental Bacillus mixture (Bacillus tequilensis YC5-2 + B. endophyticus C2-2 and YC3-B and the commercial probiotic Alibio. The DNA for PCR-SSCP analyses was extracted directly from the guts of shrimps treated for 20 days with the probiotics and injected with 2.5*10(5 CFU g-1 of V. parahaemolyticus one week after suspension of the probiotic treatment. Untreated shrimps served as positive (injected with V. parahaemolyticus and negative (not injected controls Analysis of the bacterial community carried out after inoculation and 12 and 48 h later confirmed that V. parahaemolyticus was present in shrimps of the positive control , but not in the negative control or treated with the probiotic mixtures. A significant difference in the diversity of the bacterial community was observed between times after infection. The band patterns in 0-12 h were clustered into a different group from that determined after 48 h, and suggested that during bacterial infection the guts of whiteleg shrimp were dominated by gamma proteobacteria represented by Vibrio sp. and Photobacterium sp. Our results indicate that the experimental and the commercial mixtures are suitable to modulate the bacterial community of L. vannamei and could be used as a probiotic to control vibriosis in juvenile shrimp.

  7. Monitoring of inland waters for culturing shrimp Litopenaeus vannamei: application of a method based on survival and chemical composition.

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    Valencia-Castañeda, G; Millán-Almaraz, M I; Fierro-Sañudo, J F; Fregoso-López, M G; Páez-Osuna, F

    2017-08-01

    This study evaluated the suitability of 21 inland waters (16 well waters and 5 surface waters) from Northwest Mexico via short- (48 h) and medium-term (28 days) tests using postlarvae (PL18) of Litopenaeus vannamei. In the short test, survival was assessed at 48 h after shrimp were placed in groups of 10 postlarvae into 2-L containers of inland water, to which they had been previously acclimated. The second, medium-term test consisted of four replicates with 10 postlarvae, and each group was placed in 15-L containers with the treatment water. Weights (initial and final) and survival were evaluated weekly for 28 days. In those waters for which the short test was positive and the medium-term test was negative and which also had a deficiency of potassium and/or magnesium, a third test was conducted. These last waters were supplemented with salts, and the shrimp survival and weights (initial and final) were recorded for 28 days. The water samples from San Jose, Mochicahui, Sinaloa River, Caimanero inner Lagoon, La Pipima, Campo Santa Fe, Escopama, and Fitmar had >60% survival in the short test. The Caimanero inner Lagoon water had the highest survival (87.5 ± 9.6%) and final mean weight (201.3 ± 86.2 mg). In the third test, it was found that shrimp in the water from La Pipima, Campo Santa Fe, and Fitmar exhibited 100% survival for 2 weeks. Finally, in this work, a decision tree to evaluate the suitability of low-salinity water for shrimp farming was proposed, which can be applied in other regions.

  8. Sequencing and de novo analysis of the hemocytes transcriptome in Litopenaeus vannamei response to white spot syndrome virus infection.

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    Shuxia Xue

    Full Text Available BACKGROUND: White spot syndrome virus (WSSV is a causative pathogen found in most shrimp farming areas of the world and causes large economic losses to the shrimp aquaculture. The mechanism underlying the molecular pathogenesis of the highly virulent WSSV remains unknown. To better understand the virus-host interactions at the molecular level, the transcriptome profiles in hemocytes of unchallenged and WSSV-challenged shrimp (Litopenaeus vannamei were compared using a short-read deep sequencing method (Illumina. RESULTS: RNA-seq analysis generated more than 25.81 million clean pair end (PE reads, which were assembled into 52,073 unigenes (mean size = 520 bp. Based on sequence similarity searches, 23,568 (45.3% genes were identified, among which 6,562 and 7,822 unigenes were assigned to gene ontology (GO categories and clusters of orthologous groups (COG, respectively. Searches in the Kyoto Encyclopedia of Genes and Genomes Pathway database (KEGG mapped 14,941 (63.4% unigenes to 240 KEGG pathways. Among all the annotated unigenes, 1,179 were associated with immune-related genes. Digital gene expression (DGE analysis revealed that the host transcriptome profile was slightly changed in the early infection (5 hours post injection of the virus, while large transcriptional differences were identified in the late infection (48 hpi of WSSV. The differentially expressed genes mainly involved in pattern recognition genes and some immune response factors. The results indicated that antiviral immune mechanisms were probably involved in the recognition of pathogen-associated molecular patterns. CONCLUSIONS: This study provided a global survey of host gene activities against virus infection in a non-model organism, pacific white shrimp. Results can contribute to the in-depth study of candidate genes in white shrimp, and help to improve the current understanding of host-pathogen interactions.

  9. RESPONS PERTUMBUHAN DAN EKSPRESI GEN UDANG VANAME, Litopenaeus vannamei SETELAH DIRENDAM DALAM LARUTAN HORMON PERTUMBUHAN REKOMBINAN IKAN KERAPU KERTANG

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    Siti Subaidah

    2012-12-01

    Full Text Available Pertumbuhan ikan dapat ditingkatkan menggunakan hormon pertumbuhan rekombinan. Penelitian ini bertujuan mengkaji respons pertumbuhan dan ekspresi gen udang vaname, Litopenaeus vannamei setelah direndam dengan hormon pertumbuhan rekombinan ikan kerapu kertang, Epinephelus lanceolatus (rElGH. Pada percobaan pertama, post larva stadia 2 (PL-2 sebanyak 1.500 ekor direndam selama 1 jam dalam 1 liter air laut yang mengandung rElGH lima dosis berbeda, yaitu 150; 15; 1,5; 0,15; dan 0,015 mg/L dan bovine serum albumin 0,01%. Setiap perlakuan diulang 3 kali. Perendaman dilakukan dalam kantong plastik ditambah oksigen (volume air :oksigen = 1:5. Udang dipelihara dalam akuarium volume 60 liter dengan kepadatan 25 ekor/litersampai PL-14. Hasil penelitian menunjukkan bahwa dosis 15 mg/L memberikan peningkatan bobot badan, panjang badan, dan sintasan tertinggi (P<0,05 masing-masing sebesar 37,77%; 12,75%; dan 9,45% dibandingkan dengan kontrol. Ekspresi mRNA single insulin binding domain (SIBD pada PL-14 yang dianalisis dengan realtime PCR menunjukkan kenaikan sebesar 3,3 kali pada udang yang direndam rElGH dibandingkan dengan kontrol, dan dapat dinyatakan bahwa SIBD berperan penting dalam induksi pertumbuhan. Tingkat ekspresi moult inhibiting hormone meningkat sekitar 13%, sedangkan ekspresi cyclopilin A pada udang yang direndam rElGH sama dengan kontrol. Pada percobaan kedua, perendaman PL-2 dalam larutan rElGH dosis 15 mg/L dengan lama waktu 3 jam meningkatkan bobot badan sebesar 62,18% lebih tinggi daripada perendaman 1 jam. Dengan demikian, perendaman udang dalam larutan rElGH meningkatkan pertumbuhan dan ekspresi gen SIBD, dan metode ini dapat berguna dalam peningkatan produksi budidaya.

  10. Effects of ammonia-N stress on metabolic and immune function via the neuroendocrine system in Litopenaeus vannamei.

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    Cui, Yanting; Ren, Xianyun; Li, Jian; Zhai, Qianqian; Feng, Yanyan; Xu, Yang; Ma, Li

    2017-05-01

    The purpose of this study was to evaluate the immunological responses, such as phenoloxidase (PO), antibacterial, and bacteriolytic activities, and metabolic variables, such as oxyhemocyanin, lactate, and glucose levels, of Litopenaeus vannamei exposed to ambient ammonia-N at 0, 2.5, 5, 7.5, and 10 mg/L for 0, 3, 6, 12, 24, and 48 h, and determine the effects of the eyestalk hormone on the metabolic and immune functions of unilateral eyestalk-ablated L. vannamei exposed to ambient ammonia-N at 10 mg/L. The actual concentrations of the control and test solutions were 0.04, 2.77, 6.01, 8.30, and 11.36 mg/L for ammonia-N and 0.01, 0.15, 0.32, 0.44, and 0.60 mg/L for NH3-N (unionized ammonia). The results showed a significant decrease in the PO, antibacterial, and bacteriolytic activities in the plasma as well as a significant increase in the glucose and lactate levels and decreased oxyhemocyanin levels in the hemolymph of L. vannamei exposed to elevated ammonia-N levels. These findings indicated that L. vannamei exposed to ammonia-N might demonstrate weakened metabolic and immunological responses. Moreover, eyestalk removal caused a dramatic decrease in PO, antibacterial, and bacteriolytic activities, which indicated that the eyestalk hormone in L. vannamei exhibited a higher immune response due to the induction of protective mechanisms against ammonia-N stress. Eyestalk removal also caused a dramatic decrease in glucose and lactate levels, suggesting that the eyestalk hormone is involved in glucose metabolism to meet the energy requirements under ammonia-N stress conditions. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. MKK4 from Litopenaeus vannamei is a regulator of p38 MAPK kinase and involved in anti-bacterial response.

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    Wang, Sheng; Yin, Bin; Li, Haoyang; Xiao, Bang; Lǚ, Kai; Feng, Chiguang; He, Jianguo; Li, Chaozheng

    2018-01-01

    LvMKK4, a homologue of the mammalian mitogen-activated protein kinase kinase 4 (MKK4), was isolated and identified from Litopenaeus vannamei in the present study. The full-length cDNA of LvMKK4 is 1947 bp long, with an open reading frame (ORF) of 1185 bp encoding a putative protein of 388 amino acids. LvMKK4 contains several characteristic domains such as D domain, SIAKT motif and kinase domain, all of which are conserved in MAP kinase kinase family. Like mammalian MKK4 but not Drosophila MKK4, LvMKK4 could bind to, phosphorylate and activate p38 MAPK, which provided some insights into the signal transduction mechanism of MKK4-p38 cascade in invertebrates. Our real-time PCR data indicated that LvMKK4 was ubiquitously expressed in all tested tissues and extraordinarily abundant in muscle. Dual luciferase reporter assays in Drosophila S2 cells revealed that LvMKK4 activated the transcription of antimicrobial peptide genes (AMPs), including Drosophila Attacin A, Drosomycin, and shrimp Penaeidins. Additionally, LvMKK4 was up-regulated in both intestine and hepatopancreas by a variety of inflammatory stimuli including LPS, Vibrio parahaemolyticus, Staphhylococcu saureus, Poly (I: C) and white spot syndrome virus. Furthermore, RNAi-mediated knockdown of LvMKK4 enhanced the sensitivity of L. vannamei to V. parahaemolyticus infection. These findings suggested that LvMKK4 played an important role in anti-bacterial response and could be a potential target for inflammation treatment. Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. Comparative proteome analysis of the hepatopancreas from the Pacific white shrimp Litopenaeus vannamei under long-term low salinity stress.

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    Xu, Chang; Li, Erchao; Liu, Yan; Wang, Xiaodan; Qin, Jian G; Chen, Liqiao

    2017-06-06

    Litopenaeus vannamei is a typical euryhaline decapod model to study the osmoregulation mechanism in crustaceans. The proteomic was undertaken using isobaric tags for relative and absolute quantification together with the reverse phase in high-performance liquid chromatography mass spectrometry to quantitatively identify the proteins differentially expressed in the hepatopancreas under low salinity stress (3psu) compared with the control salinity (25psu). 533 proteins and 84 differentially expressed proteins were identified including 58 proteins with the 1.2-fold cut-off value under chronically low salinity stress. Among these proteins, 26 were up-regulated while 32 were down-regulated. 48 out of 58 differentially expressed proteins were annotated in the Uniprot database and were mapped into 38 pathways by KEGG analysis. These proteins were categorized into the pathways for energy metabolism, signaling, immunization and detoxification, lipid and protein metabolism. A more active glycometabolism, positive response detoxification pathway, immunosuppression and positive osmoregulation were identified in L.vannamei under low salinity stress. This study suggests that under chronically low salinity stress, L. vannamei showed low immunity and high demand for energy especially from glycometabolism. Signaling transfer related pathways, especially the Wnt signaling pathways were involved in the process of salinity adaption, but the in-depth mechanism warrants further investigation. In this study, a comprehensive physiological response was studied using proteomics to reveal the underlying mechanism of adaptation to low salinity in L.vannamei, which was the first report on the proteomic response of crustacean to salinity stress. The extensive proteomic investigation on hepatopancreas under low salinity stress provides a new insight into the adaptive mechanism of this euryhaline crustacean species to low salinity. Copyright © 2017 Elsevier B.V. All rights reserved.

  13. Adaptation of the white shrimp Litopenaeus vannamei to gradual changes to a low-pH environment.

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    Han, Si-Yin; Wang, Bao-Jie; Liu, Mei; Wang, Meng-Qiang; Jiang, Ke-Yong; Liu, Xin-Wei; Wang, Lei

    2018-03-01

    pH variation could cause a stress response in euryhaline penaeids, we evaluated the mortality, growth performance, osmoregulation gene expression, digestive enzyme activity, histology, and resistance against Vibrio parahemolyticus of white shrimp Litopenaeus vannamei reared under conditions of gradual changes to a low-pH environment (gradual-low pH, 6.65-8.20) or a high-pH environment (gradual-high pH, 8.20-9.81) versus a normal pH environment (8.14-8.31) during a 28-d experiment. Consequently, under gradual-high pH, the cumulative mortality rate (CMR) rose with time until 39.9% on days 28; the weight gain percentage (WGP) and length gain percentage (LGP) decreased continuously. However, under gradual-low pH, the CMR of shrimp stabilized at 6.67% during 7-28 d; the WGP and LGP decreased first and then returned to normal. These results indicated that L. vannamei displayed a moderate tolerance to gradual-low pH, compared with gradual-high pH. Under gradual-low pH, the Na+/K+-ATPase, cytoplasmic carbonic anydrase (CAc), and glycosyl-phosphatidylinositol-linked carbonic anhydrase (CAg) transcripts of shrimp increased continuously or then back to normal; the amylase, lipase, and trypsin activities decreased first and then returned to normal or increased; the hepatopancreases and midguts showed histopathological lesions first and then got remission. Thus, the major adaptation mechanism of shrimp to gradual-low pH might be its high osmoregulation ability, which made shrimp achieve a new, balanced steady-state, then promoted longer intestinal villi and recuperative hepatopancreases of shrimp with enhanced digestive enzyme activities to increase nutrient absorption after long-term exposure. Meanwhile, the enhanced resistance against V. parahemolyticus under gradual-low pH would probably inhibit disease outbreak in the shrimp farming. Copyright © 2017 Elsevier Inc. All rights reserved.

  14. Isolation of Potential Bacteria as Inoculum for Biofloc Formation in Pacific Whiteleg Shrimp, Litopenaeus vannamei Culture Ponds.

    Science.gov (United States)

    Kasan, Nor Azman; Ghazali, Nurarina Ayuni; Ikhwanuddin, Mhd; Ibrahim, Zaharah

    2017-01-01

    A new green technology to reduce environmental damages while optimizing production of Pacific Whiteleg shrimp, Litopenaeus vannamei was developed known as "Biofloc technology". Microbial communities in biofloc aggregates are responsible in eliminating water exchange and producing microbial proteins that can be used as supplemented feed for L. vannamei. This study aimed to isolate and identify potential bioflocculant-producing bacteria to be used as inoculum for rapid formation of biofloc. For the purpose of this study, bacterial communities during 0, 30 and 70 days of culture (DOC) of L. vannamei grow-out ponds were isolated and identified through phenotypic and 16S rDNA sequences analysis. Phylogenetic relationships between isolated bacteria were then evaluated through phylogenetic tree analysis. One-way analysis of variance (ANOVA) was used to compare the differences of microbial communities at each DOC. Out of 125 bacterial isolates, nine species of bacteria from biofloc were identified successfully. Those bacteria species were identified as Halomonas venusta, H. aquamarina, Vibrio parahaemolyticus, Bacillus infantis, B. cereus, B. safensis, Providencia vermicola, Nitratireductor aquimarinus and Pseudoalteromonas sp., respectively. Through phylogenetic analysis, these isolates belong to Proteobacteria and Firmicutes families under the genera of Halomonas sp., Vibrio sp., Bacillus sp., Providencia sp., Nitratireductor sp. and Pseudoalteromonas sp. In this study, bioflocculant-producing bacteria were successfully identified which are perfect candidates in forming biofloc to reduce water pollution towards a sustainable aquaculture industry. Presence of Halomonas sp. and Bacillus sp. in all stages of biofloc formation reinforces the need for new development regarding the ability of these species to be used as inoculum in forming biofloc rapidly.

  15. Flightless-I (FliI) is a potential negative regulator of the Toll pathway in Litopenaeus vannamei.

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    Zhang, Shuang; Qiu, Wei; Chen, Yong-gui; Yuan, Feng-Hua; Li, Chao-Zheng; Yan, Hui; Weng, Shao-Ping; He, Jian-Guo

    2015-02-01

    Flightless-I (FliI) is a protein negatively modulates the Toll-like receptor (TLR) pathway through interacting with Myeloid differentiation factor 88 (MyD88). To investigate the function of FliI in innate immune responses in invertebrates, Litopenaeus vannamei FliI (LvFliI) was identified and characterized. The full-length cDNA of LvFliI is 4, 304 bp long, with an open reading frame (ORF) encoding a putative protein of 1292 amino acids, including 12 leucine-rich repeat (LRR) domains at the N-terminus and 6 gelsolin homology (GEL) domains at the C-terminus. The LvFliI protein was located in the cytoplasm and LvFliI mRNA was constitutively expressed in healthy L. vannamei, with the highest expression level in the muscle. LvFliI could be up-regulated in hemocytes after lipopolysaccharide (LPS), poly I:C, CpG-ODN2006, Vibrio parahaemolyticus, Staphylococcus aureus, and white spot syndrome virus (WSSV) challenges, suggesting a stimulation response of LvFliI to bacterial and immune stimulant challenges. Upon LPS stimulation, overexpression of LvFliI in Drosophila Schneider 2 cells led to downregulation of Drosophila and shrimp antimicrobial peptide (AMP) genes. Knockdown of LvFliI by RNA interference (RNAi) resulted in an increase of the expression of three shrimp AMP genes (PEN2, crustin, and Lyz1). However, the mortality rates of LvFliI-knockdown shrimp in response to V. parahaemolyticus, S. aureus or WSSV infections were not significantly different from those of the control group. Taken together, all the results suggested that LvFliI may play a negative role in TLR signaling response in L. vannamei. Copyright © 2014 Elsevier Ltd. All rights reserved.

  16. Impact of Vibrio parahaemolyticus and white spot syndrome virus (WSSV) co-infection on survival of penaeid shrimp Litopenaeus vannamei

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    Zhang, Xiaojing; Song, Xiaoling; Huang, Jie

    2016-11-01

    White spot syndrome virus (WSSV) is an important viral pathogen that infects farmed penaeid shrimp, and the threat of Vibrio parahaemolyticus infection to shrimp farming has become increasingly severe. Viral and bacterial cross or superimposed infections may induce higher shrimp mortality. We used a feeding method to infect Litopenaeus vannamei with WSSV and then injected a low dose of V. parahaemolyticus (WSSV+Vp), or we first infected L. vannamei with a low-dose injection of V. parahaemolyticus and then fed the shrimp WSSV to achieve viral infection (Vp+WSSV). The eff ect of V. parahaemolyticus and WSSV co-infection on survival of L. vannamei was evaluated by comparing cumulative mortality rates between experimental and control groups. We also spread L. vannamei hemolymph on thiosulfate citrate bile salt sucrose agar plates to determine the number of Vibrio, and the WSSV copy number in L. vannamei gills was determined using an absolute quantitative polymerase chain reaction (PCR) method. LvMyD88 and Lvakt gene expression levels were detected in gills of L. vannamei by real-time PCR to determine the cause of the diff erent mortality rates. Our results show that (1) the cumulative mortality rate of L. vannamei in the WSSV+Vp group reached 100% on day 10 after WSSV infection, whereas the cumulative mortality rate of L. vannamei in the Vp+WSSV group and the WSSV-alone control group approached 100% on days 11 and 13 of infection; (2) the number of Vibrio in the L. vannamei group infected with V. parahaemolyticus alone declined gradually, whereas the other groups showed significant increases in the numbers of Vibrio ( Pvannamei and vice versa. The combined accelerated proliferation of both V. parahaemolyticus and WSSV led to massive death of L. vannamei.

  17. Nitric oxide as an antimicrobial molecule against Vibrio harveyi infection in the hepatopancreas of Pacific white shrimp, Litopenaeus vannamei.

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    Chen, Ting; Wong, Nai-Kei; Jiang, Xiao; Luo, Xing; Zhang, Lvping; Yang, Dan; Ren, Chunhua; Hu, Chaoqun

    2015-01-01

    Nitric oxide (NO) is a key effector molecule produced in the innate immune systems of many species for antimicrobial defense. However, how NO production is regulated during bacterial infection in invertebrates, especially crustaceans, remains poorly understood. Vibrio harveyi, a Gram-negative marine pathogen, is among the most prevalent and serious threats to the world's shrimp culture industry. Its virulence typically manifests itself through shrimp hepatopancreas destruction. In the current study, we found that NO generated by an in vitro donor system (NOC-18) could rapidly and effectively kill V. harveyi. In addition, injection of heat-killed V. harveyi increased the concentration of NO/nitrite and the mRNA expression of nitric oxide synthase (NOS) in the hepatopancreas of Pacific white shrimp (Litopenaeus vannamei), the commercially most significant shrimp species. Live V. harveyi challenge also induced NO/nitrite production and NOS gene expression in primary L. vannamei hepatopancreatic cells in a time- and dose-dependent manner. Co-incubation of l-NAME, an inhibitor selective for mammalian constitutive NOSs, dose-dependently blocked V. harveyi-induced NO/nitrite production, without affecting V. harveyi-induced NOS mRNA expression. Furthermore, l-NAME treatment significantly increased the survival rate of infecting V. harveyi in cultured primary hepatopancreatic cells of L. vannamei. As a whole, we have demonstrated that endogenous NO produced by L. vannamei hepatopancreatic cells occurs in enzymatically regulated manners and is sufficient to act as a bactericidal molecule for V. harveyi clearance. Copyright © 2014 Elsevier Ltd. All rights reserved.

  18. An activating transcription factor of Litopenaeus vannamei involved in WSSV genes Wsv059 and Wsv166 regulation.

    Science.gov (United States)

    Li, Xiao-Yun; Yue, Hai-Tao; Zhang, Ze-Zhi; Bi, Hai-Tao; Chen, Yong-Gui; Weng, Shao-Ping; Chan, Siuming; He, Jian-Guo; Chen, Yi-Hong

    2014-12-01

    Members of activating transcription factor/cyclic adenosine 3', 5'-monophosphate response element binding protein (ATF/CREB) family are induced by various stress signals and function as effector molecules. Consequently, cellular changes occur in response to discrete sets of instructions. In this work, we found an ATF transcription factor in Litopenaeus vannamei designated as LvATFβ. The full-length cDNA of LvATFβ was 1388 bp long with an open reading frame of 939 bp that encoded a putative 313 amino acid protein. The protein contained a basic region-leucine zipper (bZip) domain that was a common feature among ATF/CREB transcription factors. LvATFβ was highly expressed in intestines, gills, and heart. LvATFβ expression was dramatically upregulated by white spot syndrome virus (WSSV) infection. Pull-down assay revealed that LvATFβ had strong affinity to promoters of WSSV genes, namely, wsv059 and wsv166. Dual-luciferase reporter assay showed that LvATFβ could upregulate the expression of wsv059 and wsv166. Knocked down LvATFβ resulted in decreased expression of wsv059 and wsv166 in WSSV-challenged L. vannamei. Knocked down expression of wsv059 and wsv166 by RNA interference inhibited the replication and reduce the mortality of L. vannamei during WSSV challenge inoculation. The copy numbers of WSSV in wsv059 and wsv166 knocked down group were significant lower than in the control. These results suggested that LvATFβ may be involved in WSSV replication by regulating the expression of wsv059 and wsv166. Copyright © 2014 Elsevier Ltd. All rights reserved.

  19. Involvement of dopamine beta-hydroxylase in the neuroendocrine-immune regulatory network of white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Cheng, Winton; Ka, Ya-Wen; Chang, Chin-Chyuan

    2017-09-01

    In shrimp, the biosynthesis of catecholamines, including dopamine and norepinephrine, is required for physiological and immunological responses against stress. Dopamine beta-hydroxylase (DBH), a copper-containing monooxygenase enzyme that plays an important role in catecholamine synthesis of the neuroendocrine regulatory network, was identified in Litopenaeus vannamei. In the present study, the potential role of DBH in the immunocompetence of L. vannamei was further estimated by depleting DBH by pharmaceutical inhibition of disulfiram and a gene silencing technique of L. vannamei DBH-double-stranded (ds)RNA (LvDBH-dsRNA). Immunocompetence was evaluated following the determination of the total hemocyte count, differential hemocyte count, phenoloxidase activity, respiratory bursts, superoxide dismutase activity, phagocytic activity, and the clearance efficiency as well as the susceptibility against Vibrio alginolyticus infection. At 30-120 min after shrimp had received disulfiram, they exhibited significantly reduced total hemocyte count, phenoloxidase activity of hemocytes in hemolymph, respiratory bursts of hemocytes in hemolymph and per hemocyte, phagocytic activity, clearance efficiency, and survival ratio against V. alginolyticus infection, compared to those injected with saline. In addition, the significantly lower total hemocyte count, phagocytic activity, clearance efficiency, and resistance to V. alginolyticus infection were observed in shrimp that received LvDBH-dsRNA at 3 days post injection compared to those injected with diethyl pyrocarbonate-water or non-targeting gene-dsRNA. The DBH depleted L. vannamei revealed immunosuppression and decreased the survival ratio to V. alginolyticus infection, which indicated that DBH played a crucial role in the neuroendocrine-immune regulatory network. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Combining in silico transcriptome mining and biological mass spectrometry for neuropeptide discovery in the Pacific white shrimp Litopenaeus vannamei

    Science.gov (United States)

    Ma, Mingming; Gard, Ashley L.; Xiang, Feng; Wang, Junhua; Davoodian, Naveed; Lenz, Petra H.; Malecha, Spencer R.; Christie, Andrew E.; Li, Lingjun

    2009-01-01

    The shrimp Litopenaeus vannamei is arguably the most important aquacultured crustacean, being the subject of a multi-billion dollar industry worldwide. To extend our knowledge of peptidergic control in this species, we conducted an investigation combining transcriptomics and mass spectrometry to identify its neuropeptides. Specifically, in silico searches of the L. vannamei EST database were conducted to identify putative prepro-hormone-encoding transcripts, with the mature peptides contained within the deduced precursors predicted via online software programs and homology to known isoforms. MALDI-FT mass spectrometry was used to screen tissue fragments and extracts via accurate mass measurements for the predicted peptides, as well as for known ones from other species. ESI-Q-TOF tandem mass spectrometry was used to de novo sequence peptides from tissue extracts. In total 120 peptides were characterized using this combined approach, including 5 identified both by transcriptomics and by mass spectrometry (e.g. pQTFQYSRGWTNamide, Arg7-corazonin, and pQDLDHVFLRFamide, a myosuppressin), 49 predicted via transcriptomics only (e.g. pQIRYHQCYFNPISCF and pQIRYHQCYFIPVSCF, two C-type allatostatins, and RYLPT, authentic proctolin), and 66 identified solely by mass spectrometry (e.g. the orcokinin NFDEIDRAGMGFA). While some of the characterized peptides were known L. vannamei isoforms (e.g. the pyrokinins DFAFSPRLamide and ADFAFNPRLamide), most were novel, either for this species (e.g. pEGFYSQRYamide, an RYamide) or in general (e.g. the tachykinin-related peptides APAGFLGMRamide, APSGFNGMRamide and APSGFLDMRamide). Collectively, our data not only expand greatly the number of known L. vannamei neuropeptides, but also provide a foundation for future investigations of the physiological roles played by them in this commercially important species. PMID:19852991

  1. Impact of the O2 concentrations on bacterial communities and quality of modified atmosphere packaged Pacific white shrimp (Litopenaeus vannamei).

    Science.gov (United States)

    Qian, Yun-Fang; Yang, Sheng-Ping; Xie, Jing; Xiong, Qing; Gao, Zhi-Li

    2013-12-01

    The importance of spoilage-related bacteria in fresh Pacific white shrimp (Litopenaeus vannamei) under different modified atmospheres (MAs) at 4 °C and the effect of O2 were demonstrated in the current study. The changes of bacterial communities in MA-packed shrimp during cold storage were studied by a combined method of plate counts with isolation and identification. Three gas mixtures were applied: 80% CO2 /5% O2 /15% N2, 80% CO2 /10% O2 /10% N2 and 80% CO2 /20% O2, and unsealed packages of shrimp were used as the control. In addition, the TVB-N, pH, whiteness index, and sensory scores were also determined to evaluate the quality changes of shrimp. MA packaging effectively inhibited the increase of total psychrotrophic bacteria counts and H2 S-producing bacteria counts by about 1.7 and 2.1 log cycles, respectively. The growth of Gram-negative spoilage bacteria in shrimp, including Shewanella spp., Aeromonas spp., and Pseudomonas spp., was inhibited by MA packaging, but the growth rate of Gram-positive bacteria such as lactic acid bacteria (LAB) and Brochothrix spp. were less affected by MA as effectively as Gram-negative bacteria. In comparison with the MA-packaged samples, the counts of H2 S producers in shrimp under a CO2 -enriched atmosphere with 20% O2 were slightly lower than the count in samples under an atmosphere with 5% O2 . However, MA with 20% O2 led to higher concentrations of TVB-N, and lower whiteness values and sensory scores. The shelf life of shrimp under 80% CO2 /10% O2 /10% N2 has been prolonged by > 6 d in comparison with the control according to the sensory scores. © 2013 Institute of Food Technologists®

  2. Hemolymph cells apoptosis in imported shrimp Litopenaeus vannamei from Hawaii to Iran, exposed to white spot virus

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    Zeliha Selamoglu Talas

    2014-07-01

    Full Text Available Objective: To show hemolymph apoptosis in imported shrimp Litopenaeus vannamei from Hawaii to Iran. Methods: One hundred and eighty shrimps [(7.98±0.54 g] which were collected from a research shrimp farm located in Heleh site in north of Bushehr Province were distributed equally to 6 glass aquariums (50 cm×50 cm×60 cm as group A in triplicate (imported batch in 2011, without crossing with other generations with well clean aerated sea water (100 L per aquarium, salinity of 40 ‰ and temperature of 29 °C. Shrimps of group B (produced by crossing the adults of imported batches in 2009 up to 2011 were distributed also among 6 aquariums with the same conditions. Both shrimp groups were injected with concentration of LD50=1×10 5.4 white spot virus. Results: The results showed that in group A, the mortality began approximately 24 h after exposure and reached 100% after 36 h but no mortality was occurred up to 15 d in shrimps of group B. The slide evaluation of hemolymph of group B showed an increasing trend of apoptosis occurrence in all three types of hemolymph cells, hyalinocytes, semi-granulocytes and granulocytes from 24 h to 72 h in contrary to group A that not any apoptosis was observed during the course of the study (15 d. Conclusions: It is concluded that crossing among the specific pathogen free generations could induce the increasing immunity level through apoptosis to protect them against white spot disease.

  3. Comparison of white spot syndrome virus infection resistance between Exopalaemon carinicauda and Litopenaeus vannamei under different salinity stresses

    Science.gov (United States)

    Ge, Qianqian; Yu, Ge; Sun, Ming; Li, Jitao; Li, Jian

    2017-12-01

    Exopalaemon carinicauda is one of the important economic shrimp species in China, and can tolerate a wide range of salinities. However, its disease resistance remains to be unclear in comparison with other shrimp species under salinity stress. In this study, the resistance to white spot syndrome virus (WSSV) of E. carinicauda and Litopenaeus vannamei was determined by comparing their hemocyanin (Hc) and phenoloxidase (PO) activities under different salinity stresses. In E. carinicauda, the PO activity and Hc gene transcript abundance showed a coherent pattern of increase and decrease while Hc content showed a slightly decrease with Vibrio anguillarum and WSSV infections. For both E. carinicauda and L. vannamei under salinity stress, the PO activity showed a positive correlation with the salinity while the Hc content and expression level of its gene increased significantly in salinities of 5, 15 and 25 g L-1. The survival rate of E. carinicauda with WSSV infection was higher than that of L. vannamei in the first 24 h under different salinity stresses. Drastic mortality of E.carinicauda and L. vannamei appeared at 48 h and 3 h post-injection, respectively. Furthermore, compared with L. vannamei, E. carinicauda displayed higher PO activity, Hc content and abundance of Hc gene mRNA. The results collectively indicated that Hc and PO have obviously functional connection in resisting pathogens and tolerating salinity stress, and PO activity and Hc gene mRNA abundance may reflect the resistance of shrimp to disease. E. carinicauda has higher level of immune potential than L. vannamei, suggesting its greater capacity in resisting pathogens under salinity stresses.

  4. Investigating the biometric and physicochemical characteristics of freshly harvested Pacific white shrimp (Litopenaeus vannamei): a comparative approach.

    Science.gov (United States)

    Okpala, Charles Odilichukwu R; Bono, Gioacchino

    2016-03-15

    The practicality of biometrics of seafood cannot be overemphasized, particularly for competent authorities of the shrimp industry. However, there is a paucity of relevant literature on the relationship between biometric and physicochemical indices of freshly harvested shrimp. This work therefore investigated the relationship between biometric (standard length (SL), total weight (TW) and condition factor (CF)) and physicochemical (moisture content, pH, titratable acidity, water activity, water retention index, colour values and fracturability) characteristics of freshly harvested Pacific white shrimp (Litopenaeus vannamei) obtained from three different farms. The relationships between these parameters were determined using correlation and regression analyses. No significant correlation (P > 0.05) was found between the biometric and physicochemical indices of the sampled L. vannamei specimens. Possibly the lack of post-mortem and physical change(s) at day of harvest together with the absence of temporal variable may have collectively limited the degree of any significant correlation between biometric and physicochemical data points measured in this study. Although the TWs of freshly harvested L. vannamei shrimp resembled (P > 0.05), SL and CF differed significantly (P physicochemical variables were independently correlated (P physicochemical characteristics of freshly harvested L. vannamei shrimp. Across the farms studied, however, the biometric data were comparable. To best knowledge, this is the first study to investigate the biometric and physicochemical properties of freshly harvested shrimp using a comparative approach, which is also applicable to other economically important aquaculture species. Overall, this work provides useful information for competent authorities/stakeholders of the fishery industry and serves as a baseline for preservative treatments. © 2015 Society of Chemical Industry.

  5. Ecología trófica del jabalí europeo (Sus scrofa silvestre en Chile Trophic ecology of the wild boar (Sus scrofa in Chile

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    ÓSCAR SKEWES

    2007-09-01

    Full Text Available Documentamos la dieta del jabalí silvestre en el centro-sur de Chile basados en el examen de 20 estómagos colectados en los faldeos de volcán Mocho-Choshuenco (39°54' S, 72°02' O y en el Parque Nacional Vicente Pérez Rosales (41°03' S, 71°54' O. La dieta está representada por animales, vegetales y hongos. Entre los vegetales y hongos son consumidas tanto las partes epígeas como hipógeas, lo mismo que frutos y semillas. Hongos y rizomas de Gunnera tinctoria constituyen ítemes vegetales cuantitativamente importantes en la alimentación. El jabalí forrajea en general en ambiente boscoso, pero las especies vegetales más frecuentes en la dieta se encuentran en espacios abiertos o fuera del bosque. El ambiente dominado por Chusquea spp. constituye un importante sitio de alimentación. Los roedores de la familia Muridae dominan entre los animales determinados en la dieta del jabalí. Esta situación, sin embargo, parece obedecer a la ocurrencia de una ratada producto de la semillación masiva de Chusquea spp. durante la época de muestreo. Las aves de la familia Rhinocryptidae e invertebrados (en especial larvas del coleóptero Chiasognathus grantii constituyen parte frecuente de los ítemes animales de la dieta del jabalí. En comparación a estudios en otras partes del mundo, el jabalí silvestre en Chile consume hongos en porcentaje de ocurrencia similar (65 % a su dieta en Estados Unidos de América pero bastante más alta que su dieta en Europa (5-32 %. En relación al volumen del componente animal, el jabalí en Chile consume más (16,1 % que en Estados Unidos de América (6 % y que en Europa (7-13 %We document the diet of wild boar in south-central Chile based on analysis of 20 stomachs collected on the outskirts of Mocho-Choshuenco volcano (39°54' S, 72°02' W and of Vicente Pérez Rosales National Park (41°03' S, 71°54' W. The diet incorporates animals, plants and fungi. Among the latter two, both epigeal and hypogeal parts

  6. Frutos tropicais silvestres e polpas de frutas congeladas: atividade antioxidante, polifenóis e antocianinas Wild fruits and pulps of frozen fruits: antioxidant activity, polyphenols and anthocyanins

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    Eugenia Marta Kuskoski

    2006-08-01

    Full Text Available Os sucos de frutas tropicais conquistam cada vez mais o mercado consumidor, sendo o Brasil um dos principais produtores. Existe grande diversidade de produtos derivados de frutos e constante inserção de novos produtos no mercado de consumo, os quais, na maioria das vezes, ainda não foram devidamente pesquisados com respeito às suas propriedades e atividades benéficas à saúde. Neste trabalho, objetiva-se determinar algumas propriedades de frutos tropicais silvestres in natura e polpas de frutos comercializados congelados. Para determinar a atividade antioxidante, utilizou-se o método do radical 2,2-difeniL-1-picrilhidrazilo (DPPH·; para determinar os polifenóis totais, o método de Folin-Ciocalteu e, para as antocianinas, o método da diferença de pH. As polpas de frutas analisadas foram de amora, uva, açaí, goiaba, morango, acerola, abacaxi, manga, graviola, cupuaçu e maracujá; e os frutos silvestres, jambolão e baguaçu. Representada em TEAC (atividade antioxidante equivalente a Trolox, a atividade antioxidante global das polpas oscila entre mínimos e máximos 0,5 e 53,2mimol g-1, enquanto que, representada em VCEAC (atividade antioxidante equivalente a vitamina C, entre mínimos e máximos de 64,8 e 1198,9mg100g-1. Para os extratos de polpa dos frutos in natura, a atividade antioxidante oscila entre 13,3 e 111,2mimol g-1 (TEAC e entre 42,8 e 2533,1mg 100g-1 (VCEAC. Em ordem decrescente de capacidade antioxidante, encontra-se: acerola> manga> morango> uva> açaí> goiaba> amora> graviola> maracujá> cupuaçu> abacaxi. Os frutos de baguaçu apresentam maior atividade antioxidante que os de jambolão.The tropical fruit juices are gaining ever greater space in the consumer market, and Brazil is one of the main producer countries in this market. There is a great diversity of products derived from fruits and new products for consumption are launched constantly, often without the necessary research into their active properties and

  7. [Population viability of Alouatta palliata (Primates: Atelidae) and Cebus capucinus (Primates: Cebidae) at Refugio de Vida Silvestre Privado Nogal, Sarapiquí, Heredia, Costa Rica].

    Science.gov (United States)

    Rodríguez-Matamoros, Jorge; Villalobos-Brenes, Federico; Gutiérrez-Espeleta, Gustavo A

    2012-06-01

    Habitat destruction may cause wildlife population fragmentation and is considered an important factor in small population species extinction. As wildlife populations become smaller, threats to their stability and persistence arise as a result of demographic, environmental and genetic stochastic factors. The aim of this work was to study the effects of population fragmentation on the long term viability of Alouatta palliata and Cebus capucinus populations, at Refugio de Vida Silvestre Privado Nogal, Sarapiquí (RVSPN), Heredia. For this we used the computer software VORTEX to run a population viability analysis (PVA) for both species. The input data of the PVA were taken from the demography structure of the RVSPN, literature sources from the species and from PVA related papers. We evaluated two sets of scenarios: small fragmented populations to reflect the population current state, and one larger and continuous population, to reflect the effect of reforestation actions followed by RVSPN to connect forest fragments. Results suggest that both A. palliata and C. capucinus can survive in isolated forest fragments. However, if different factors as inbreeding depression, catastrophes or habitat loss were incorporated to the scenarios, the small fragmented populations become unstable and the risk of extinction increased for both species. Continuous and larger populations were more robust against the threats incorporated in the scenarios when compared to the current situation of smaller and fragmented populations. The best management option for both species would be to continue reforestation efforts in the area to connect forest fragments, with the result of larger and continuous populations of both species. It is important to continue the observation of both species populations, and to promote a habitat management to reduce the negative effects of stochastic environmental events.

  8. Associação entre isoenzimas e matéria seca em batata silvestre Isoenzymes and dry matter content in wild potato

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    BEATRIZ HELENA GOMES ROCHA

    2000-12-01

    Full Text Available Clones de batata silvestre (Solanum spp. foram analisados quanto aos teores de matéria seca em tubérculos e isoenzimas de aspartato transaminase e isocitrato desidrogenase em folhas, com o objetivo de identificar associação entre estas características. Os resultados obtidos em uma amostra de 50 clones indicam grande variabilidade do teor de matéria seca. Clones de S. commersonii malmeanum apresentaram maior teor que os de S. commersonii commersonii, S. chacoense muelleri e de outros cuja espécie não foi identificada. Os padrões isoenzimáticos de folhas de 38 clones da amostra mostraram 15 variantes eletroforéticas de aspartato transaminase e sete de isocitrato desidrogenase. Comparações efetuadas através de tabelas de contingência 2 x 2, usando o teste de chi², com correção para continuidade, permitiram concluir que existe associação significativa entre o teor de matéria seca e as bandas de mobilidade relativa 1,00 de aspartato transaminase e de isocitrato desidrogenase.Wild potato clones (Solanum spp. were analysed for tuber dry matter content, leaf aspartate transaminase and leaf isocitrate dehydrogenase isoenzymes in order to identify association between these characteristics. The results indicate that there is great variability for dry matter content among 50 clones analysed. S. commersonii malmeanum clones had higher dry matter levels than S. commersonii commersonii, S. chacoense muelleri and the clones whose species were not identified. Fifteen aspartate transaminase and seven isocitrate dehydrogenase bands were observed in the gels of 38 clones. Tests of chi², using 2 x 2 contingency table, revealed a significant association between dry matter content and the bands of relative mobility 1.00 of both aspartate transaminase and isocitrate dehydrogenase isoenzymes.

  9. Observações sobre calazar em Jacobina, Bahia. VI - Investigações sobre reservatórios silvestres e comensais

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    I. A. Sherlock

    1988-03-01

    Full Text Available Durante os anos de 1982 a 1986, a investigação sobre mamíferos comensais e silvestres, da periferia da cidade de Jacobina, Bahia, mostrou, ao lado do escasso número de exemplares, uma reduzida variedade específica dessa fauna. Capturou-se apenas 11 espécies, entre as quais, predominou o Didelphis albiventris, que abrangeu 44% dos 213 espécimens capturados. Entre os 193 com exames já concluídos, 84 eram exemplares de D. albiventris e 2 estavam infectados pela Leishmania donovani senso lato, 1 por L. mexicana amazonensis, 1 por L. braziliensis, subespécie e 3 por Trypanosoma cruzi Também foram observadas formas suspeitas de serem amastigotas de leishmanics, nos esfregaços de órgãos de 3 exemplares de Dasyprocta aguti, 1 Cercomys cunicularius - e 1 Oryzomys eliurus. 0 restante dos exemplares, inclusive 14 de Lycalopex vetulus, estava negativo para flagelados. Apesar de reforçado por outros indicadores epidemiológicos, como a predominância específica, a freqüência domiciliar, a atratividade para a vetora Lutzomyia longipalpis, e a concomitância com casos humanos nos mesmos locais, o índice de 2,3% de infecção natural do Didelphis albiventris, não autoriza a conclusão definitiva de ser o marsupial o mais importante reservatório natural da leishmaniose visceral em Jacobina.

  10. Ecología del Paisaje. Un marco para el estudio integrado de la dinámica territorial y su incidencia en la vida silvestre

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    Gurrutxaga San Vicente, Mikel

    2008-12-01

    Full Text Available A review on the conceptual basis and aims of Landscape Ecology, scientific discipline that presents an important geographical component, is realized. It assumes that landscape heterogeneity controls diverse movements and flows of organisms, matter and energy. One of the principal objects of study of the above mentioned discipline is the existing relations between landscape structural patterns and dynamics of wild populations and communities. This is an aspect of great applicability in biodiversity conservation and land-use planning policies. In relation to this subject matter, transdisciplinarity of Landscape Ecology is described.

    Se realiza una revisión sobre las bases conceptuales y los objetivos de la Ecología del Paisaje, disciplina científica que presenta un importante componente geográfico. Como punto de partida asume que la heterogeneidad espacio-temporal del paisaje, resultante de la interacción dinámica de las sociedades humanas con el medio, controla diversos movimientos y flujos de organismos, materia y energía. Uno de los principales objetos de estudio de dicha disciplina son las relaciones existentes entre los cambios estructurales del paisaje y la dinámica de poblaciones y comunidades silvestres, configurándose como un aspecto de gran aplicabilidad en las políticas de conservación de la biodiversidad y de planificación territorial con base ecológica. En relación a esta temática, se describe el carácter transdisciplinar de la Ecología del Paisaje.

  11. Pasado y presente del uso de plantas silvestres con órganos subterráneos de almacenamiento comestibles en la Patagonia

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    Juan J. Ochoa

    2011-07-01

    Full Text Available El presente trabajo es la primara aproximación acerca del papel de las plantas con órganos de almacenamiento subterráneos comestibles (POAS en la Patagonia, desde una perspectiva temporal y espacial, al integrar datos etnohistóricos y etnográficos actuales ligados al uso de las POAS. El 75 % de las 54 fuentes analizadas mencionan el uso de POAS en la alimentación humana de la Patagonia. Las POAS silvestres comestibles registradas ascienden a 51 especies, siendo mayor el conjunto de POAS con raíces comestibles. La estepa y zonas ecotonales resultaron las de mayor riqueza de POAS, probablemente vinculado a adaptaciones ecológicas de éstas a la aridez en el primer caso, como a la alta diversidad relativa que presentan los ecotonos, en el segundo. El registro etnohistórico mostró discontinuidades marcadas, siendo pocas las especies presentes por más de un siglo, destacándose la continuidad documental de A. tuberosa desde el siglo XVIII al presente, así como la incorporación reciente de especies exóticas. Las fuentes etnohistóricas revisadas y su contraste con datos etnobotánicos recientes de campo sugieren dinamismo en los patrones de utilización, que a lo largo del tiempo han llevado al abandono del uso de las POAS en la práctica alimentaria de la Patagonia. Las singularidades encontradas respecto a lo temporal y espacial pueden vincularse tanto a los conocimientos locales y específicos desarrollados a lo largo del tiempo por los habitantes de la Patagonia, como también a las circunstancias sociopolíticas y académicas de cada una de las fuentes analizadas.

  12. ESTRATÉGIAS QUE CONTRIBUEM PARA A IDENTIFICAÇÃO E CONTENÇÃO DE SURTO DE FEBRE AMARELA SILVESTRE NO GOIÁS

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    Thiago Vinicius N. de Menezes

    2010-08-01

    Full Text Available O foco desse estudo foi apresentar ferramentas que proporcionam tanto a identificação como a contenção e controle dos casos de Febre Amarela Silvestre No Goiás. Este estudo teve por base a análise de dados de morbimortalidade por febre amarela e imagens de satélite (geoprocessamento de pontos do Goiás. Os dados foram disponibilizados pela Secretaria de Vigilância em Saúde/MS, visando o conhecimento das estatísticas relacionadas à doença, das estratégias de controle adotadas nas três instâncias da saúde focando principalmente nas medidas de preservação de posteriores epidemias. Vários fatores são identificados através da análise das imagens obtidas através do satélite onde se vê claramente a crescente amplitude que as cidades estão tomando a ponto de degradar o ambiente natural da doença. Medidas são adotadas pelo Ministério da Saúde, a intensificação da vacinação contra a febre amarela para todas as pessoas, acima de seis meses de idade, orientação aos serviços de saúde para que notifiquem de imediato às autoridades sanitárias os casos atendidos e ou internados de pacientes com quadro clínico suspeito de febre amarela, entre outras. As imagens obtidas por geoprocessamento favoreceram na tomada de decisões para a prevenção e controle da doença.

  13. Parásitos gastrointestinales y ectoparásitos de ungulados silvestres en condiciones de vida libre y cautiverio en el trópico mexicano

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    José Manuel Mukul-Yerves

    2014-01-01

    Full Text Available Se identificaron los parásitos gastrointestinales y ectoparásitos presentes en venados cola blanca ( Odocoileus virginianus , pecarí de collar ( Pecari tajacu y venado temazate ( Mazama americana en condiciones de vida libre y cautiverio en el trópico mexicano. Se obtuvieron muestras fecales y ectoparásitos de 12 ungulados de vida libre que se cazaron para fines de subsistencia y de 55 ungulados en condiciones de cautiverio. Las muestras de excremento se analizaron mediante las pruebas de Flotación Centrifugada y McMaster para conocer los géneros y órdenes de parásitos que afectan a los ungulados; así como la excreción de huevos y ooquistes por gramo de excremento. Los ectoparásitos encontrados se identificaron taxonómicamente. Las muestras positivas a nematodos del orden Strongylida y protozoos del orden Eucoccidiorida se cultivaron para su identificación taxonómica a nivel de géneros. En venados cola blanca y temazate en cautiverio se identificaron los géneros Strongyloides, Trichuris, Capillaria, Mammomonogamus y Eimeria; mientras que en los pecaríes de collar los géneros Oesophagostomun, Eimeria e Isospora . En los tres ungulados de vida libre se identificó el género Strongyloides. Sólo se encontraron ectoparásitos en ungulados de vida libre, identificándose la pulga Pulex irritans y el piojo Gliricola porcelli en venados temazate y pecaríes de collar, mientras que la mosca Lipoptena sp se colectó en los venados cola blanca. La garrapata Amblyomma cajennese se encontró parasitando a las tres especies de ungulados estudiados. Se concluye que los ungulados silvestres del presente estudio estuvieron parasitados con nematodos gastrointestinales, protozoos del orden Eucoccidiorida, pulgas, piojos, moscas y garrapatas.

  14. Epidemiología molecular de la rabia urbana en Colombia, 1994-2004. La rabia en ecosistemas silvestres como amenaza para la población humana

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    Jorge Boshell

    2004-03-01

    Full Text Available

    En Colombia la rabia ocurre en dos ciclos epidemiológicos principales: la rabia urbana con el perro como principal reservorio y transmisor principalmente a humanos, y la rabia silvestre con especies animales de la vida silvestre como los quirópteros y zorros entre otros, como reservorios y transmisores principalmente a especies ganaderas y eventualmente a humanos que se convierten en víctimas accidentales. Durante los últimos años la rabia silvestre en Colombia ha cobrado mayor relevancia como problema de salud pública que la rabia urbana, a juzgar por el número de víctimas humanas. Con el fin de perfeccionar el diagnóstico y la tipificación del virus de la rabia en Colombia, el Laboratorio Nacional de Referencia del Instituto Nacional de Salud estandarizó técnicas para amplificar y secuenciar un fragmento de ADN complementario (cADN a una fracción de 902 nucleótidos seleccionados del ARN del virus, con el fin de adelantar estudios de epidemiología molecular. La fracción mencionada contiene secuencias que codifican para los aminoácidos 447-525 de la glicoproteína y 1-35 de la proteína L. Además contiene la región intergénica no codificante conocida como Pseudogen Psi.

    Las técnicas estandarizadas fueron las siguientes: a extracción de ARN total a partir de cerebro de ratón infectado. b amplificación molecular por transcripción reversa y reacción en cadena de la polimerasa. c secuenciación del fragmento amplificado.

    Mediante estas técnicas de epidemiología molecular viral se determinó que durante 1994-2004, la rabia urbana se concentró en tres regiones geográficas definidas, con epizootias en el antiplano cundiboyacense, departamento de Arauca y la región Caribe. Las dos primeras se controlaron

  15. Diversidad y aspectos biológicos de abejas silvestres de un ambiente urbano y otro natural de la región central de Santa Fe, Argentina

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    Milagros DALMAZZO

    2010-01-01

    Full Text Available El objetivo del presente trabajo fue relevar el elenco de abejas silvestres en la región central de Santa Fe y comparar la diversidad de especies y aspectos biológicos en dos ambientes de una misma región. Las abejas fueron colectadas en el estrato herbáceo de la Reserva Universitaria “Escuela Granja” de la localidad Esperanza, y en la zona urbana de la localidad Susana, ambas ubicadas dentro de la Provincia Fitogeográfica del Espinal. La colecta se realizó en octubre, noviembre y diciembre de 2004 y en enero, febrero y marzo de 2005, registrando las especies vegetales sobre las que se capturaron. Se colectaron 277 ejemplares en total, de los cuales se identificaron 62 especies (27 géneros en 5 familias. Las curvas de rarefacción indican una tasa de acumulación de especies mayor para la Reserva Ecológica, los índices de diversidad de Shannon son 3,25 para la Reserva Ecológica y 2,64 para la Zona Urbana. Para la comparación de las comunidades en ambos sitios, las abejas fueron clasificadas en tres categorías según la especialización en el uso del recurso floral, sustrato de nidificación y comportamiento social. Se observó una asociación entre los grupos especialistas y de nidificación en el suelo con el ambiente natural, y por otro lado, las especies generalistas y de nidificación en cavidades con el ambiente urbano. No se identificaron diferencias, en cuanto al tipo de comportamiento social de las especies, entre ambos sitios.

  16. PROECHIMYS SEMISPINOSUS (RATÓN DE ESPINAS: UNA ESPECIE DE FAUNA SILVESTRE CON POTENCIAL PROMISORIO PARA COMUNIDADES NEGRAS DEL DEPARTAMENTO DEL CHOCÓ, PACÍFICO COLOMBIANO

    Directory of Open Access Journals (Sweden)

    Asprilla-Perea Jeferson

    2012-12-01

    Full Text Available Se valoró el potencial de Proechimys semispinosus (ratón de espinas como especiepromisoria para el departamento del Chocó, según su uso tradicional en comunidadesnegras y a través de ensayos de laboratorio como aporte nutricional de su carne. Laalimentación es el principal uso que los pobladores de estas comunidades le dana la especie, y la trampa de cajón es la técnica de cacería que más se utiliza parasu captura. El sabor de la carne de este roedor goza de buena aceptación por partede sus consumidores, y no se han asociado enfermedades humanas a la ingesta dela misma. A nivel socioeconomico, aunque es una importante fuente de proteinapara las comunidades, su carne no se comercializa con frecuencia. Los ensayosde laboratorio mostraron que su carne posee características bromatológicas (20%proteínas, 78% humedad, 0.40% grasas, 0.97% Cenizas y 3.34 Kcal/100 g similaresa carnes domésticas de origen porcino, vacuno y aviar, así como también a otrassilvestres como la guagua (Cuniculus paca y el cuy (Cavia porcellus. Por lotanto, P. semispinosus es una especie de fauna silvestre con potencial promisoriopara comunidades negras en el departamento del Chocó, ya que es ampliamenteutilizado como alimento en localidades rurales de la región (que es la mayor partedel territorio y su carne cuenta con un importante valor nutricional

  17. Evaluación de la resistencia al pasador del fruto Neoleucinodes elegantalis y caracterización morfoagronómica de germoplasma silvestre de Lycopersicon spp.

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    Restrepo S Edwin Fernando

    2006-03-01

    Full Text Available COMPENDIO En un diseño de bloques completos al azar con tres repeticiones se evaluaron diez materiales de L. hirsutum, dos de L. peruvianum y uno de L. escu­lentum cv maravilla (testigo. El 60.3% de los frutos fueron afectados por N. elegantalis en el testigo; mientras que en las accesiones de L. hirsutum y L. peruvianum ningún fruto fue afectado. Todas las accesiones silvestres presentaron resistencia de campo. Los coeficientes de variación de los descriptores cuantitativos usados en la evaluación morfoagronómica, fluctuaron entre 22.88 y 51.89%. El análisis de clasificación con los descriptores cuantitativos, permitió la conformación de cuatro grupos y de tres con los cualitativos. Palabras claves: descriptores, Neoleucinodes elegantalis, materiales. ABSTRACT Resistance evaluation to the fruit borer N. elegantalis and Morphoagronomic characterization in wild germplasm of Lycopersicon spp. The percentage of affected fruits with borer .N. elegantalis in the control treatment (L. esculentum cv maravilla was of 60.3%; whereas in all the evaluated maternals of wild species L. hirsutum and L. peruvianum, none fruit was affected by this plague insect. All the wild materials of Lycopersicon spp showed a resistance of fieldâ€? to the fruit borer. The variation coefficients of the quantitative descriptors used in the morpho agronomic evaluation fluctuated between 23.02 and 58.40%, indicating that thet genetic potential available in this germplasm evalu­ated for breeding programs, depends on the character that it'is looking to improve. The analysis of classification with quantitative and qualitative descriptors allowed with both the formation of four and three groups in wich the similar genetic constitution of the materials in each group was the probable cause of the obtained classification. Key words: Lycopersicon wild species descriptors, genetic constitution. Neoleucinodes elegantalis

  18. Análisis de la dinámica de poblaciones silvestres de Chigüiros Hydrochaeris hydrochaeris (Linnaeus, 1766 como herramienta de manejo hacia el aprovechamiento sostenible y la conservación

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    Elizabeth Mesa González

    2006-01-01

    Full Text Available Se analizó el comportamiento de la dinámica de poblaciones silvestres de chigüiros bajo diferentes condiciones del hábitat y presiones antrópicas, y se formuló una herramienta técnica para apoyar la gestión de manejo que realiza la autoridad ambiental regional relacionada con el aprovechamiento sostenible y la conservación de la especie. Se generó un modelo de simulación determinístico que representa la dinámica de poblaciones silvestres de chigüiros, como resultado de procesos biológicos de natalidad y mortalidad, y del aprovechamiento extractivo anual de individuos en hatos de los municipios de Paz de Ariporo y Hato Corozal (Casanare, Colombia. Una interfase fue diseñada para facilitar el uso del modelo y construir escenarios para simular diferentes alternativas de manejo. El modelo fue usado para evaluar el efecto a largo plazo de la cosecha o extracción de individuos mediante la caza comercial, la cosecha selectiva de adultos por sexos, la presencia de adecuaciones y la variación del área de hábitat adecuado disponible sobre la tendencia de abundancia de tres poblaciones silvestres de chigüiros en la zona de estudio. Las simulaciones realizadas bajo los diferentes escenarios permitieron establecer las distintas respuestas de cada población a los cambios realizados en la tasa de cosecha y el área de hábitat y así recomendar acciones de manejo. Los escenarios evaluados destacan la importancia de hacer una extracción selectiva de adultos por sexos para realizar un aprovechamiento sostenible de la población y garantizarsu conservación. Así mismo, se destaca la importancia de mantener mayores áreas con hábitat adecuado.

  19. Análisis de la dinámica de poblaciones silvestres de Chigüiros Hydrochaeris hydrochaeris (Linnaeus, 1766 como herramienta de manejo hacia el aprovechamiento sostenible y la conservación

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    López Arévalo Hugo Fernando

    2006-06-01

    Full Text Available Se analizó el comportamiento de la dinámica de poblaciones silvestres de chigüiros bajo diferentes condiciones del hábitat y presiones antrópicas, y se formuló una herramienta técnica para apoyar la gestión de manejo que realiza la autoridad ambiental regional relacionada con el aprovechamiento sostenible y la conservación de la especie. Se generó un modelo de simulación determinístico que representa la dinámica de poblaciones silvestres de chigüiros, como resultado de procesos biológicos de natalidad y mortalidad, y del aprovechamiento extractivo anual de individuos en hatos de los municipios de Paz de Ariporo y Hato Corozal (Casanare, Colombia. Una interfase fue diseñada para facilitar el uso del modelo y construir escenarios para simular diferentes alternativas de manejo. El modelo fue usado para evaluar el efecto a largo plazo de la cosecha o extracción de individuos mediante la caza comercial, la cosecha selectiva de adultos por sexos, la presencia de adecuaciones y la variación del área de hábitat adecuado disponible sobre la tendencia de abundancia de tres poblaciones silvestres de chigüiros en la zona de estudio. Las simulaciones realizadas bajo los diferentes escenarios permitieron establecer las distintas respuestas de cada población a los cambios realizados en la tasa de cosecha y el área de hábitat y así recomendar acciones de manejo. Los escenarios evaluados destacan la importancia de hacer una extracción selectiva de adultos por sexos para realizar un aprovechamiento sostenible de la población y garantizarsu conservación. Así mismo, se destaca la importancia de mantener mayores áreas con hábitat adecuado.

  20. Determinación de anticuerpos contra patógenos virales y bacterianos seleccionados en la población de cerdos silvestres (Sus scrofa de la Reserva Natural Bahía Samborombón, Argentina

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    B. Carpinetti

    2017-06-01

    Full Text Available Los cerdos silvestres (Sus scrofa descienden de cruzamientos entre cerdos domésticos liberados durante la colonización con jabalíes salvajes euroasiáticos, liberados con propósitos cinegéticos. Son invasivos y su coexistencia con especies domésticas implica riesgos sanitarios. Argentina es considerada libre de fiebre aftosa (FA, peste porcina clásica (PPC y africana (PPA y síndrome reproductivo y respiratorio porcino (PRRS. La enfermedad de Aujeszky (EA y la leptospirosis son endémicas en ciertas áreas del país. El objetivo fue evaluar la presencia de ciertas enfermedades zoonóticas y/o de importancia para la producción animal y la conservación de la biodiversidad en cerdos silvestres de la Bahía de Samborombón. Se capturaron 118 animales. Se tomaron muestras de suero, tonsilas, músculo, intestino delgado, linfonódulos, entre otras. Se estudió la presencia de anticuerpos contra Brucella spp., coronavirus respiratorio porcino, virus de la estomatitis vesicular, de la FA, de la gastroenteritis transmisible porcina (TGEV, de la PPC, PPA, EA, PRRS y Leptospira spp. Se realizaron análisis bacteriológicos para Mycobacterium spp. Los resultados ratificaron la ausencia de las enfermedades exóticas e indicaron que 36 % de los animales presentó anticuerpos contra Leptospira interrogans serovar pomona y 62,5 % contra el virus de la EA. Estos resultados remarcan la importancia del monitoreo de la interfase productiva/silvestre en función de la salud pública, producción animal y conservación de la biodiversidad.

  1. Descripción del ciclo de vida de una población silvestre de Triatoma dimidiata (Hemiptera: Rediviidae) y análisis genético de tres grupos por medio de marcadores bioquímicos

    OpenAIRE

    Ardila Roldán Susanne Carolina

    2002-01-01

    El insecto de la subfamilia Reduviidae, Triatoma dimidiata, es actualmente en Colombia elsegundo vector más importantes en la transmisión de la enfermedad de Chagas luego de laespecie Rhodnius prolixusquien se halla principalmente en viviendas humanas. Este insecto puedehabitar viviendas rústicas, peridomicilios o sitios aledaños a las viviendas o ambientes silvestres,insectos de los cuales se conoce poco sobre su capacidad vectorial y de infestación. Para es-tudiar algunas aspectos de la bio...

  2. Frecuencia de Varroa destructor, Nosema apis y Acarapis woodi en colonias manejadas y enjambres silvestres de abejas (Apis mellifera en Mérida, Yucatán, México

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    Jesús Froylán Martínez Puc

    2011-01-01

    Full Text Available Las enfermedades y parasitosis que afectan a las abejas melíferas causan importantes pérdidas económicas a la actividad apícola. Sin embargo, los daños provocados por dichas parasitosis se presentan de manera heterogénea en colonias manejadas y en enjambres silvestres. Con la finalidad de determinar la frecuencia y niveles de infestación de V. destructor, N. apis y A. woodi en abejas melíferas en Yucatán, se colectaron un total de 76 muestras de abejas melíferas, durante junio a septiembre de 2006, siendo 27 de colonias manejadas y 49 de enjambres silvestres. La frecuencia de V. destructor en colonias manejadas fue de 62.9 %, con un nivel de infestación de 1.70 ± 0.26 (ácaros/100 abejas, y en los enjambres silvestres fue de 55.1 %, con un nivel de infestación de 1.96 ± 0.44. No se observaron diferencias en la frecuencia (X2 = 0.44, gl = 1, P =0.51, y niveles de infestación (t=0.14, P=0.89. La frecuencia de N. apis en las colonias manejadas fue de 74.0 %, con una media de infestación de 1´480 x 103 ± 232 x 103 (esporas/ abeja, y en los enjambres silvestres de 53.0 %, con una media de infestación de 1´416 x 103 ± 264 x 103, no se observaron diferencias en la frecuencia (X2 = 3.22, gl = 1, P= 0.07 y niveles de infestación (t=0.18, P=0.86. No se detectó la presencia de A. woodi en las muestras analizadas. Los resultados demuestran un aumento en la frecuencia de N. apis en Yucatán.

  3. Ocorrência de anticorpos anti-Toxoplasma gondii em aves silvestres de três Unidades de Conservação Federais da Paraíba e da Bahia

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    Leontina H.M. Andrade

    2016-02-01

    Full Text Available Resumo: A vigilância e monitoramento de doenças em animais silvestres são imprescindíveis no contexto ambiental e de saúde pública, pois estes animais agem como sentinelas, refletindo alterações ambientais precocemente, o que proporciona maior eficácia no monitoramento ambiental e permite o acesso rápido a informações sobre as condições da área. Neste contexto, as aves são importantes no ciclo biológico do Toxoplasma gondii e na epidemiologia da toxoplasmose, principalmente porque seus tecidos representam importantes fontes de proteína na alimentação de felídeos e humanos. Objetivou-se detectar anticorpos anti-T. gondii, por meio do teste de aglutinação modificada em aves silvestres de três Unidades de Conservação (UC Federais dos Estados da Paraíba e Bahia. No período de dezembro de 2011 a outubro de 2013 foram capturadas com redes de neblina 222 aves silvestres pertencentes a 67 espécies, 27 famílias e 12 ordens. Após a captura, foi colhido sangue de cada animal e separado o soro, que foi submetido ao Teste de Aglutinação Modificada (MAT≥1:25 utilizando taquizoítos inativados na formalina e 2-mercaptoetanol. Dentre as 222 amostras analisadas, três (1,3% foram sororreagentes: 1 de 16 (6,2% pipira-preta Tachyphonus rufus (título 50, 1 de 5 (20% juriti-gemedeira Leptotila rufaxilla (título 50 e 1 de 1 (100% caneleiro-enxofre Casiornis fuscus (título 25. Este é o primeiro relato da ocorrência de anticorpos anti-T. gondii nas referidas espécies de aves silvestres de vida livre nas duas UC Federais estudadas.

  4. Estudio Retrospectivo del Ingreso de Animales Provenientes del Tráfico Ilegal a la Unidad de Rescate y Rehabilitación de Animales Silvestres (URRAS), entre febrero de 1996 y agosto de 2006

    OpenAIRE

    Sandra Milena Lamprea-Maldonado; P A Moreno; O Sánchez; Ximena Gómez; Nelson Jurado

    2009-01-01

    Este estudio da a conocer las cifras de animales provenientes del  tráfico y tenencia ilegal que ingresaron a la Unidad de Rescate y Rehabilitación de Animales Silvestres  (URRAS) de la Facultad de Medicina Veterinaria y Zootecnia de la Universidad Nacional de Colombia, en el período comprendido entre Febrero de 1996 y Agosto de 2006. A partir de la información recopilada en la base de datos y actas de ingreso, se establecieron las principales taxas afectadas por el ilícito, tipos de ingreso ...

  5. Identificación Estructural de Compuestos Mayoritarios en Plantas Silvestres de Castilleja tenuiflora Benth y su Acumulación en Cultivos de Raíces in vitro.

    OpenAIRE

    Gomez Aguirre, Yenny Adriana

    2015-01-01

    En este estudio, por primera vez se identificaron el verbascósido e isoverbascósido como los principales feniletanoides glicosilados (FG) en plantas silvestres de Castilleja tenuiflora. Se identificaron dos iridoides glucosilados (IG) aucubina y bartsiósido, así como el flavonoide apigenina. Se ha demostrado la actividad biológica de esos compuestos, como anti-inflamatoria, antioxidante y citotóxica, que pueden estar relacionadas con los usos tradicionales de esta planta. Se de...

  6. Acesso ao alimento artificial e enchimento do trato digestivo de juvenis do camarão marinho Litopenaeus vannamei (Boone (Crustacea, Decapoda, Penaeidae durante as fases clara e escura do período de 24 horas Artificial food access and digestive tract filling of juvenil marine shrimp Litopenaeus vannamei (Boone (Crustacea, Decapoda, Penaeidae during light and dark phases in 24-hour period

    Directory of Open Access Journals (Sweden)

    Cibele S. Pontes

    2005-12-01

    Full Text Available A escassez de dados acerca do comportamento alimentar do camarão marinho Litopenaeus vannamei (Boone, 1931 com relação ao alimento artificial ofertado em bandejas poderá induzir a uma alimentação inadequada, resultando no desperdício da ração, no aporte desnecessário de nutrientes, e conseqüente aumento dos custos com a alimentação e da poluição ambiental potencial desta atividade. Objetivando fornecer subsídios para a melhoria do manejo alimentar praticado nas fazendas, foram realizados estudos comportamentais utilizando 64 juvenis de Litopenaeus vannamei (7,57 ± 1,01g, mantidos em densidade populacional de 33 m², submetidos a fotoperíodo artificial, em ciclo invertido, para observação durante as fases clara e escura. A ração foi ofertada em intervalos pré-estabelecidos, registrando-se posteriormente através de método focal contínuo: a latência para chegada à bandeja, b latência para consumo do alimento e c Índice de Enchimento do Trato Digestivo. Os animais foram mais rápidos para chegar à bandeja e também para iniciar o consumo do alimento nos horários da fase clara. O enchimento do trato digestivo mostrou-se superior na meia hora subsequente à oferta do alimento, especialmente nos horários da fase clara.The few data on the marine shrimp Litopenaeus vannamei (Boone, 1931 behavior related to feed offer in trays can result inadequate feeding by the animal, feed wastage and unnecessary nutrient input, increasing feed costs and potential environmental pollution of that culture. In order to provide tools for improved feed management methods in shrimp farms, a behavioral study was conducted, using 64 Litopenaeus vannamei juveniles (7,57 ± 1,01g, one animal per 33 m². They were submitted to artificial photoperiods, half of them in reversed cycle, in order to register behavior during light and dark phases. The following variables were registered (continuous sampling after feed exposition: a latency to access the

  7. Biorremediación de los efluentes de cultivo del camarón Litopenaeus vannamei (Boone, 1931 utilizando tapetes microbianos en un sistema de recirculación Bioremediacion of effluents ones of the culture of Litopenaeus vannamei (Boone, 1931 using microbial mats in a recirculating system

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    Carlos Lezama-Cervantes

    2010-01-01

    Full Text Available Se desarrolló un sistema de recirculación (SR integrando el cultivo del camarón con tapetes microbianos buscando mitigar los impactos ambientales de los subproductos del cultivo del camarón y avanzar hacia la biorremediación del agua de cultivo. Los consorcios microbianos obtenidos de ambientes naturales (TaM estuvieron constituidos por bacterias (55,6%, cianófitas (18,4%, diatomeas (9%, nemátodos (5,6% y clorófitas (1,4%, entre los grupos taxonómicos principales. La remoción de nutrientes y sólidos se evaluó en un SR (2 ciclos/hora conteniendo (n = 3 60 y 120 ind m-2 de Litopenaeus vannamei. Los resultados demostraron que los TaM redujeron los niveles de nitrógeno amoniacal por encima del 71% diariamente; la demanda bioquímica de oxígeno (DBO5 se redujo más de 68% y los sólidos suspendidos (SST hasta en 62% al compararse con los sistemas control (p 0,05. El agua bio-remediada tiene un efecto positivo en el cultivo de Litopenaeus vannamei, y promueve el crecimiento y sobrevivencia en presencia de TaM en el SR.We developed a recirculating system integrating shrimp culture with microbial mats aiming to mitigate the environmental impacts of by-products from shrimp culture and to advance toward the bioremediation of the shrimp culture water. The microbial consortia collected from natural environments (TaM was constituted by bacteria (55.6%, Cyanophyte (18.4%, diatoms (9%, nematods (5.6% and Chlorophyte algae (1.4% among the major taxonomic groups. The removal of nutrients and solids by these mats was assessed in a recirculating system (2 cycles/hour (n = 3 containing 60 and 120 ind m-2 of Litopenaeus vannamei. Results showed that the microbial mat reduces daily up to 71% of ammonia nitrogen, decreases around 68% of the biochemical oxygen demand (DBO5 and up to 62% of suspended solids (SST when comparing to the control systems (p 0.05. The Bioremediated culture water had a positive effect on the culture of Litopenaeus vannamei, and

  8. Aclimatação do camarão marinho Litopenaeus vannamei (Boone, 1931 à água doce com diferentes estratégias de alimentação e calagem = Acclimatization of the marine shrimp Litopenaeus vannamei (Boone, 1931 to the freshwater with different feeding and liming strategies

    Directory of Open Access Journals (Sweden)

    Paulo de Paula Mendes

    2006-01-01

    Full Text Available Pós-Larvas do camarão Litopenaeus vannamei, na fase de PL9-10 e PL25-26 foram aclimatadas à água doce (0,0 ‰, objetivando promover cultivos em regiões interioranas. Para isso, foram realizados dois experimentos conduzidos em aquários com capacidade útil de 44 litros e densidade de estocagem de 11,36 pós-larvas/L. No experimento 1, foram testadas diferentes concentrações de cal hidratada (0,018 g a 0,031 g e 2 dietas, formuladas à base de ração comercial para camarão e biomassa de artêmia. No experimento 2, foram testadas 5dietas com diferentes concentrações de biomassa de artêmia (0% a 100%, inseridas em rações de peixes e camarões. No final do processo de aclimatação, a sobrevivência das póslarvas variou de 40,44% a 98,04%. Concluiu-se que pós-larvas do Litopenaeus vannamei podem ser aclimatadas à água doce e que o aumento da concentração de cal e de artêmia na ração melhoraram significativamente (pLitopenaeus vannamei postlarvae with 9-10 (PL9-10, 25-26 days old (PL25-26 were acclimated to freshwater (0 ppt for the purpose of attending the inland cultures. Two experiments were conducted in 44 liter aquariums with stocking density of 11.36 postlarvae/L. In experiment 01, different concentrations of hydrated lime (0.018 g a 0.031 g and two diets, formulated with commercial shrimp ration and artemia biomass, were tested. In experiment 02, five diets, withdifferent concentrations of artemia biomass (0 to 100% inserted in fish and shrimp rations, were tested. At the end of the acclimatization process, the postlarvae survival varied from40.44 to 98.04%. Results showed that Litopenaeus vannamei postlarvae can be acclimated to freshwater. Also, the increase of lime concentration and the artemia biomass in the ration improved (p<0.05 the zootechnic parameters of the species in a significant way.

  9. Genetic assessment of residual feed intake as a feed efficiency trait in the Pacific white shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Dai, Ping; Luan, Sheng; Lu, Xia; Luo, Kun; Meng, Xianhong; Cao, Baoxiang; Kong, Jie

    2017-08-04

    Residual feed intake (RFI) was investigated as a measure of feed efficiency in a breeding population of Litopenaeus vannamei. Shrimp from 34 families were housed individually and feed efficiency and growth traits were recorded during two successive growth periods. The objectives of this study were (1) to estimate the heritability of RFI and related traits, including feed efficiency ratio (FER), average daily gain (ADG) and daily feed intake (DFI), (2) to determine the relationships between RFI and other traits, and (3) to evaluate the variation of these traits across two growth periods. Shrimp displayed large inter-individual variation in RFI, FER, ADG and DFI during each growth period. Heritability estimates of all these traits during both periods reached high values (0.577 ± 0.232 to 0.707 ± 0.252). RFI showed weak and no genetic correlations with ADG during the two growth periods between days 1 to 21 (0.135 ± 0.204) and 22 to 42 (-0.018 ± 0.128), respectively, but high positive genetic correlations with DFI (>0.8). Weak and moderate negative genetic correlations were observed between RFI and FER during the two periods (-0.126 ± 0.208 and -0.387 ± 0.183). As evidenced by the high genetic correlations between the two periods for each trait (>0.6), trait performance of the shrimp tended to be consistent across periods. For the first time, accurate measurement of individual feed efficiency on a large scale was achieved in shrimp. Although the estimated heritability reported here for RFI may be overestimated, it is a heritable trait in L. vannamei that can be improved by genetic improvement. For L. vannamei, the biggest potential advantage in using RFI as a measure of feed efficiency is that it is independent of growth rate, and thus genetic selection on RFI has the potential to improve feed efficiency and reduce feed intake, without compromising growth performance.

  10. L-3,4-Dihydroxyphenylalanine (l-DOPA) induces neuroendocrinological, physiological, and immunological regulation in white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Mapanao, Ratchaneegorn; Kuo, Hsin-Wei; Chang, Chin-Chuan; Liu, Kuan-Fu; Cheng, Winton

    2018-01-03

    L-3,4-Dihydroxyphenylalanine (l-DOPA) is a precursor for dopamine (DA) synthesis. Assessments were conducted to analyze the effects of l-DOPA on mediating regulation of neuroendocrinological, immunological, and physiological parameters in the shrimp, Litopenaeus vannamei when they were individually injected with 0.01 N HCl or l-DOPA at 0.5 or 1.0 μmol shrimp -1 for 60, 120, and 240 min. For catecholamine synthesis evaluation, tyrosine hydroxylase (TH) and DA beta hydroxylase (DBH) activities, l-DOPA, DA, and norepinephrine (NE) levels in hemolymph were determined. The total hemocyte count (THC), differential hemocyte count (DHC), phenoloxidase (PO) activity, respiratory bursts (RBs), superoxide dismutase (SOD) activity, phagocytic activity, and clearance efficiency in response to the pathogen, Vibrio alginolyticus were assessed for immune responses, and plasma glucose and lactate levels were for physiological response. Results showed that the TH activity, THC, hyaline cells (HCs), and semigranular cells (SGCs) at 120 min, DA levels at 60-240 min, PO activity in hemocytes per 50 μL of hemolymph at 60-120 min, and PO activity per granulocyte (granular cells (GCs) + SGCs) at 60 min significantly increased, but TH activity, l-DOPA levels, GCs, SGCs, and respiratory bursts in hemocytes per 10 μL of hemolymph at 60 min, respiratory bursts per hemocyte and SOD activity at 120 min, phagocytic activity at 60-240 min, and the clearance efficiency at 60-120 min significantly decreased in shrimp injected with l-DOPA at 1.0 μmol shrimp -1 . In another experiment, 60 min after shrimp had received l-DOPA at 0.5 or 1.0 μmol shrimp -1 , they were challenged with an injection of V. alginolyticus at 2 × 10 5  colony-forming units (cfu) shrimp -1 . The injection of l-DOPA at 1.0 μmol shrimp -1 also significantly increased the cumulative mortality of shrimp by 16.7%, compared to the HCl-challenged control after 120 h. These results suggest

  11. The microsporidian Enterocytozoon hepatopenaei is not the cause of white feces syndrome in whiteleg shrimp Penaeus (Litopenaeus) vannamei.

    Science.gov (United States)

    Tangprasittipap, Amornrat; Srisala, Jiraporn; Chouwdee, Saisunee; Somboon, Montagan; Chuchird, Niti; Limsuwan, Chalor; Srisuvan, Thinnarat; Flegel, Timothy W; Sritunyalucksana, Kallaya

    2013-07-15

    The microsporidian Enterocytozoon hepatopenaei was first described from Thailand in 2009 in farmed, indigenous giant tiger shrimp Penaeus (Penaeus) monodon. The natural reservoir for the parasite is still unknown. More recently, a microsporidian closely resembling it in morphology and tissue preference was found in Thai-farmed, exotic, whiteleg shrimp Penaeus (Litopenaeus) vannamei exhibiting white feces syndrome (WFS). Our objective was to compare the newly found pathogen with E. hepatopenaei and to determine its causal relationship with WFS. Generic primers used to amplify a fragment of the small subunit ribosomal RNA (ssu rRNA) gene for cloning and sequencing revealed that the new parasite from WFS ponds had 99% sequence identity to that of E. hepatopenaei, suggesting it was conspecific. Normal histological analysis using tissue sections stained with hematoxylin and eosin (H&E) revealed that relatively few tubule epithelial cells exhibited spores, suggesting that the infections were light. However, the H&E results were deceptive since nested PCR and in situ hybridization analysis based on the cloned ssu rRNA gene fragment revealed very heavy infections in tubule epithelial cells in the central region of the hepatopancreas in the absence of spores. Despite these results, high prevalence of E. hepatopenaei in shrimp from ponds not exhibiting WFS and a pond that had recovered from WFS indicated no direct causal association between these infections and WFS. This was supported by laboratory oral challenge trials that revealed direct horizontal transmission to uninfected shrimp but no signs of WFS. The microsporidian newly found in P. vannamei is conspecific with previously described E. hepatopenaei and it is not causally associated with WFS. However, the deceptive severity of infections (much greater than previously reported in P. monodon) would undoubtedly have a negative effect on whiteleg shrimp growth and production efficiency and this could be exacerbated by the

  12. Fucoidan effectively provokes the innate immunity of white shrimp Litopenaeus vannamei and its resistance against experimental Vibrio alginolyticus infection.

    Science.gov (United States)

    Kitikiew, Suwaree; Chen, Jiann-Chu; Putra, Dedi Fazriansyah; Lin, Yong-Chin; Yeh, Su-Tuen; Liou, Chyng-Hwa

    2013-01-01

    In this study, we examined the effect of fucoidan on the immune response of white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus infection. Fucoidan induced degranulation, caused changes in the cell morphology, and increased activation of prophenoloxidase (proPO) and the production of superoxide anions in vitro. Shrimp that received fucoidan via immersion at 100, 200, and 400 mg l(-1) after 3 h showed haemocyte proliferation and a higher mitotic index of haematopoietic tissue. In another experiment, the haemocyte count, phenoloxidase (PO) activity, and respiratory bursts (RBs) were examined after the shrimp had been fed diets containing fucoidan at 0 (control), 0.5, 1.0, and 2.0 g kg(-1) for 7-21 days. Results indicated that these parameters directly increased with time. The immune parameters of shrimp fed the 1.0 g kg(-1) diet were significantly higher than those of shrimp fed the 2.0 g kg(-1) diet after 14 and 21 days. Phagocytic activity and the clearance efficiency against V. alginolyticus were significantly higher in shrimp fed the 1.0 g kg(-1) diet compared to those of shrimp fed the 0, 0.5 and 2.0 g kg(-1) diets. In a separate experiment, shrimp that had been fed diets containing fucoidan for 21 days were challenged with V. alginolyticus at 10(6) colony-forming units shrimp(-1). Survival rates of shrimp fed the 1.0 and 2.0 g kg(-1) diets were significantly higher than those of shrimp fed the 0 and 0.5 g kg(-1) diets for 96-120 h. We concluded that fucoidan provokes innate immunity of shrimp as evidenced by haemocyte degranulation, proPO activation, and the mitotic index of haematopoietic tissue, and that dietary administration of fucoidan at 1.0 g kg(-1) enhanced the immune response of shrimp and their resistance against V. alginolyticus infection. Copyright © 2012 Elsevier Ltd. All rights reserved.

  13. Effect of dissolved oxygen on swimming ability and physiological response to swimming fatigue of whiteleg shrimp (Litopenaeus vannamei)

    Science.gov (United States)

    Duan, Yan; Zhang, Xiumei; Liu, Xuxu; Thakur, Dhanrajsingh N.

    2013-11-01

    The swimming endurance of whiteleg shrimp (Litopenaeus vannamei, 87.66 mm ± 0.25 mm, 7.73 g ± 0.06 g) was examined at various concentrations of dissolved oxygen (DO, 1.9, 3.8, 6.8 and 13.6 mg L-1) in a swimming channel against one of the five flow velocities (v 1, v 2, v 3, v 4 and v 5). Metabolite contents in the plasma, hepatopancreas and pleopods muscle of the shrimp were quantified before and after swimming fatigue. The results revealed that the swimming speed and DO concentration were significant factors that affected the swimming endurance of L. vannamei. The relationship between swimming endurance and swimming speed at various DO concentrations can be described by the power model (ν·t b = a). The relationship between DO concentration (mg L-1) and the swimming ability index (SAI), defined as SAI = Σ{0/9000} vdt(cm), can be described as SAI = 27.947 DO0.137 (R 2 = 0.9312). The level of DO concentration directly affected the physiology of shrimp, and exposure to low concentrations of DO led to the increases in lactate and energetic substrate content in the shrimp. In responding to the low DO concentration at 1.9 mg L-1 and the swimming stress, L. vannamei exhibited a mix of aerobic and anaerobic metabolism to satisfy the energetic demand, mainly characterized by the utilization of total protein and glycogen and the production of lactate and glucose. Fatigue from swimming led to severe loss of plasma triglyceride at v 1, v 2, and v 3 with 1.9 mg L-1 DO, and at v 1 with 3.8, 6.8 and 13.6 mg L-1 DO, whereas the plasma glucose content increased significantly at v 3, v 4 and v 5 with 3.8 and 6.8 mg L-1 DO, and at v 5 with 13.6 mg L-1 DO. The plasma total protein and hepatopancreas glycogen were highly depleted in shrimp by swimming fatigue at various DO concentrations, whereas the plasma lactate accumulated at high levels after swimming fatigue at different velocities. These results were of particular value to understanding the locomotory ability of whiteleg

  14. Litopenaeus vannamei activating transcription factor 6 alpha gene involvement in ER-stress response and white spot symptom virus infection.

    Science.gov (United States)

    Yuan, Kai; He, Hong-Hui; Zhang, Chao-Zheng; Li, Xiao-Yun; Weng, Shao-Ping; He, Jian-Guo; Chen, Yi-Hong

    2017-11-01

    A previous study found that inositol-requiring enzyme-1-X-box binding protein 1 (IRE1-XBP1) pathway and the protein kinase RNA (PKR)-like ER kinase-eIF2α (PERK-eIF2α) pathway of shrimp play roles in the unfolded protein response (UPR). And they also be proved that was involved in white spot symptom virus (WSSV) infection. Yet the functions of the third branch in shrimp UPR are still unclear. In this study, we showed that upon UPR activation, activating transcription factor 6 alpha (LvATF6α) of Litopenaeus vannamei was cleaved and transferred from the cytoplasm to the nucleus in 293T cells, indicating that the ATF6 pathway in shrimp is also a branch of UPR. Furthermore, LvATF6α could reduce the apoptosis rate of Drosophila Schneider 2 (S2) cells treated with actinomycin, and knock-down expression of LvATF6α increased the apoptosis rate of shrimp hemocytes. In vivo testing revealed that the short from LvATF6α (LvATF6α-s) was obviously increased after UPR activation or WSSV infection, indicating that the ATF6 pathway was activated in L. vannamei gills under such circumstances. Moreover, knock-down expression of LvATF6α could reduce the cumulative mortality and WSSV copy number in WSSV-infected shrimp. Further study revealed that WSSV may profit from shrimp ATF6 pathway activation in two aspects. First, LvATF6α-s significantly upregulated the expression of the WSSV genes (wsv023, wsv045, wsv083, wsv129, wsv222, wsv249, and wsv343). Second, LvATF6α-s inhibited apoptosis by negatively regulating the apoptosis signal-regulating kinase 1 - (c-Jun N-terminal kinase) pathway. All of these evidences suggested that the ATF6 pathway is a member of the L. vannamei UPR, and it is also engaged in WSSV infection. Copyright © 2017 Elsevier Ltd. All rights reserved.

  15. The microsporidian Enterocytozoon hepatopenaei is not the cause of white feces syndrome in whiteleg shrimp Penaeus (Litopenaeus) vannamei

    Science.gov (United States)

    2013-01-01

    Background The microsporidian Enterocytozoon hepatopenaei was first described from Thailand in 2009 in farmed, indigenous giant tiger shrimp Penaeus (Penaeus) monodon. The natural reservoir for the parasite is still unknown. More recently, a microsporidian closely resembling it in morphology and tissue preference was found in Thai-farmed, exotic, whiteleg shrimp Penaeus (Litopenaeus) vannamei exhibiting white feces syndrome (WFS). Our objective was to compare the newly found pathogen with E. hepatopenaei and to determine its causal relationship with WFS. Results Generic primers used to amplify a fragment of the small subunit ribosomal RNA (ssu rRNA) gene for cloning and sequencing revealed that the new parasite from WFS ponds had 99% sequence identity to that of E. hepatopenaei, suggesting it was conspecific. Normal histological analysis using tissue sections stained with hematoxylin and eosin (H&E) revealed that relatively few tubule epithelial cells exhibited spores, suggesting that the infections were light. However, the H&E results were deceptive since nested PCR and in situ hybridization analysis based on the cloned ssu rRNA gene fragment revealed very heavy infections in tubule epithelial cells in the central region of the hepatopancreas in the absence of spores. Despite these results, high prevalence of E. hepatopenaei in shrimp from ponds not exhibiting WFS and a pond that had recovered from WFS indicated no direct causal association between these infections and WFS. This was supported by laboratory oral challenge trials that revealed direct horizontal transmission to uninfected shrimp but no signs of WFS. Conclusions The microsporidian newly found in P. vannamei is conspecific with previously described E. hepatopenaei and it is not causally associated with WFS. However, the deceptive severity of infections (much greater than previously reported in P. monodon) would undoubtedly have a negative effect on whiteleg shrimp growth and production efficiency and

  16. Roles of receptor for activated protein kinase C1 for modulating immune responses in white shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Chang, Zhong-Wen; Chang, Chin-Chyuan

    2015-10-01

    Complementary (c)DNA encoding a receptor for activated protein kinase C1 (RACK1) messenger (m)RNA of the white shrimp Litopenaeus vannamei, designated LvRACK1, consisted a 1136-bp cDNA containing an open reading frame (ORF) of 954 bp, a 111-bp 5'-untranslated region (UTR), and a 71-bp 3'-UTR, which is a 36 kDa cytosolic protein, belonging to the Trp-Asp40 (WD40) family of proteins, characterized by containing seven highly conserved Trp-Asp40 (WD40) internal repeats, and a poly A tail. The WD repeat of LvRACK1 can be predicted to form a seven-bladed propeller structure with each WD repeat composed of four antiparallel β-sheets. The WD40 domains have been implicated in protein-protein interactions. A comparison of amino acid sequences showed that LvRACK1 was closely related to arthropods RACK1. LvRACK1 cDNA was synthesized in all tested tissues detected with real-time PCR including haemocytes, hepatopancreas, gills, muscles, subcuticular epithelium, intestines, abdominal nervous ganglia, thoracic nervous ganglia, lymphoid organ, stomach, heart, and antennal gland, especially in subcuticular epithelium and gill. LvRACK1 mRNA transcription in haemocytes of L. vannamei injected with Vibrio alginolyticus decreased. The depletion of LvRACK1 of haemocytes in L. vannamei received its dsRNA revealed the increased respiratory bursts per haemocyte, superoxide dismutase (SOD), activity, glutathione peroxidase (GPx) activity, and clotting time, but showed the decreased total haemocyte count (THC), hyaline cells (HCs), phagocytic activity, and transglutaminase (TG) activity. LvRACK1 silenced shrimp showed the upregulated gene expressions of cyMnSOD, mtMnSOD, peroxinectin (PE), and TGI, and showed the downregulated α2-macroglobulin (α2-M), clottable protein (CP), lysozyme, and crustin gene expressions. It is therefore concluded that LvRACK1 is involved in immune defense and signaling transduction in haemocytes of L. vannamei infected with V. alginolyticus. Copyright © 2015

  17. Identification of ecdysteroid signaling late-response genes from different tissues of the Pacific white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Qian, Zhaoying; He, Shulin; Liu, Tao; Liu, Yongjie; Hou, Fujun; Liu, Qiao; Wang, Xianzong; Mi, Xiao; Wang, Ping; Liu, Xiaolin

    2014-06-01

    Ecdysteroids initiate signaling along multiple pathways that regulate various aspects of development, maturation, and reproduction in arthropods. This study was carried out to seek the late target genes of ecdysteroid signaling from different tissues of the Pacific white shrimp, Litopenaeus vannamei. In the present study, eight isoforms of ecdysteroid receptor (EcR), two isoforms of retinoic acid X receptor (RXR), and one homolog of E75 were characterized from L. vannamei. The overall protein sequences and specific functional sites of EcR, RXR and E75 among crustacean species were found highly conserved. Tissue-specific, development stage-specific, and molt stage-specific expression patterns of LvEcR, LvRXR, and LvE75 were detected by qPCR. Double stranded RNA (dsRNA)-mediated RNA interference (RNAi) of any one of the three genes LvEcR, LvRXR and LvE75 caused specific expression changes of the other two, and resulted in corresponding expression changes of two molting related genes Cathepsin-L (LvCHSL) and Hemocyanin (LvHCyn) in the hepatopancreas, two chitin metabolism related genes chitin synthase (LvChS) and chitinase isoenzyme (LvChi2) in the epidermis, and two muscle growth related genes LvActin and myosin heavy chain (LvMHC) in the muscle. In correspondence, after in vivo injections of 20 hydroxyecdysone, specific expression changes of LvEcR, LvRXR, LvE75, LvCHSL and LvHCyn in the hepatopancreas, LvEcR, LvRXR, LvE75, LvChS and LvChi2 in the epidermis, and LvEcR, LvRXR, LvE75, LvActin and LvMHC in the muscle were also observed, respectively. Results in our study indicate multiple functions of ecdysteroids signaling in L. vannamei and the function may be time- and space-specific; ecdysteroids may act through different pathways via its functional receptor heterodimer EcR-RXR and the early responsive gene E75 to perform specific regulation roles on the target genes in different shrimp tissues; LvCHSL and LvHCyn in the hepatopancreas, LvChS and LvChi2 in the

  18. Apparent carbohydrate and lipid digestibility of feeds for whiteleg shrimp, Litopenaeus vannamei (Decapoda: Penaeidae, cultivated at different salinities

    Directory of Open Access Journals (Sweden)

    Milena Gucic

    2013-09-01

    Full Text Available Whiteleg shrimp, Litopenaeus vannamei is one of the most commercially farmed species worldwide because of its fast growth, good survival rate at high farming densities, and osmoregulatory capacity, which makes it an excellent candidate for cultures at different salinities. The knowledge of shrimp nutritional requirements is critical in the formulation of diets to allow optimal growth at different environmental conditions and development stages. The effect of salinity on apparent digestibility of shrimp feed is not well known, and this information is required in shrimp diet formulation. For this purpose, the apparent digestibility coefficients of carbohydrates (ACD and lipids (ALD were determined for juvenile whiteleg shrimps under controlled culture conditions. We evaluated the apparent digestibility of six commercial (D1:37CP, D2:38CP, D3:39CP, D4:34CP, D5:35CP, and D6:37CP and two experimental (E1:33CP and E2:33CP diets for juvenile whiteleg shrimp cultivated at three salinities (5, 35 and 50psu in 60L aquariums. ACD and ALD were determined in vivo using chromic oxide as an inert marker. Our results showed that ALD in most cases was over 80%, independent of salinity, except the E1:33CPdiet which had 74.0% at 50psu. Diet D3:39CP showed the highest ALD coefficient (90.1 and 90.6% at 5 and 35psu, respectively. For ACD, differences were detected between commercial and experimental diets at every salinity level, although salinity effect on ACD was not significant. Diet D4:34CP had the highest coefficient (92.4% at 5psu, and E2:33CP at 35 and 50psu (97.3 and 94.7%. This study demonstrated that there is no significant effect of saline variations on carbohydrate and lipid digestibility by juvenile whiteleg shrimp, under the experimental conditions. Rev. Biol. Trop. 61 (3: 1201-1213. Epub 2013 September 01.

  19. Tratamiento de efluentes del cultivo de Litopenaeus vannamei por sedimentación y filtración por la ostra Crassostrea rhizophorae Treatment of Litopenaeus vannamei farming effluents by sedimentation and oyster Crassostrea rhizophorae filtration

    Directory of Open Access Journals (Sweden)

    Roberto Ramos

    2008-01-01

    Full Text Available A escala de laboratorio se evaluaron las eficiencias de remoción del material particulado y nutrientes disueltos desde efluentes de cultivo de Litopenaeus vannamei, utilizando un sistema combinado de dos fases, sedimentación y filtración por la ostra Crassostrea rhizophorae, empleando tres tiempos de retención hidráulica (TRH del efluente, 6, 12 y 24 h. En la fase de sedimentación, se emplearon estanques de 100 L en los cuales fueron colocados 90 L de efluente. En la fase de filtración, se utilizaron estanques de 50 L en los cuales se colocaron 20 L del efluente sobrenadante correspondiente a cada TRH de la fase de sedimentación. Cada unidad experimental de la fase de filtración fue sembrada con 15 ejemplares adultos de ostras con un peso promedio de 80 g manteniendo una biomasa de 1.200 g estanque-1 . Se utilizó un estanque control para cada TRH, conteniendo solamente conchas de ostras sin animal. Los resultados indicaron que en la fase de sedimentación el mejor desempeño fue alcanzado en el tiempo de 24 h, con 56,1% de remoción para la turbidez, 36,3% para los sólidos volátiles totales, la DB0(5 con 31,0% y el amonio con 21,4%. En la fase de filtración, la mayor eficiencia de remoción fue alcanzada en el tiempo de 6 h, con valores de 62,1% para la turbidez, 69,4% para los sólidos disueltos totales, 35,4% para los sólidos volátiles totales, 100% para la clorofila a y 17,2% para la DB0(5, en relación a los valores del estanque control. En esta fase, el amonio presentó un aumento en su concentración en todos los TRH. Calculadas las eficiencias de remoción con las dos fases integradas: sedimentación y filtración, en el tiempo de 6 h, en relación al efluente bruto, el desempeño mejoró significativamente alcanzando valores de 69,3% para la turbidez, 69,0% para los sólidos disueltos totales, 41,3% para los sólidos volátiles totales y 100% para la clorofila a.Efficiency in removing particulate matter and dissolved

  20. Anatomia do nervo isquiático em mocós (Kerodon rupestris WIED, 1820 aplicada a clínica de animais silvestres

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    Renata Celis dos Santos

    2006-10-01

    Full Text Available Para conhecer a origem do nervo isquiático de mocós (Kerodon rupestris WIED,1820 junto aos forames intervertebrais e a musculatura envolvida em seu trajeto, foram utilizados 10 animais adultos, oriundos do Centro de Multiplicação de Animais Silvestres da Escola Superior de Agricultura de Mossoró (CEMAS-ESAM. Após o óbito natural, estes foram fixados em formol a 10%, e foram dissecados para exposição e visualização do nervo isquiático. Os resultados foram expressos em percentual. Foram verificadas variações na quantidade de vértebras lombares e sacrais. Cinco animais (50% apresentaram sete vértebras lombares e três sacrais, dois (20% apresentaram sete vértebras lombares e quatro sacrais, e dois (20% apresentaram seis vértebras lombares e três sacrais. Um animal (10% apresentou seis vértebras lombares e quatro sacrais. Portanto, a origem do nervo foi diferenciada. Cinco animais (50% tiveram a participação de L7, S1, S2; dois animais (20% L7, S1, com pequena contribuição de S2. Dois animais (20%, de L6, S1, S2; e um animal (10%, de L6, S1 com uma pequena contribuição de S2. A última raiz do nervo isquiático em todas as suas origens, contribuiu para a formação da primeira raiz do nervo pudendo. Constatou-se que ao longo de seu trajeto os nervos isquiáticos cederam ramos para os músculos glúteo médio, glúteo profundo, glúteo surpeficial, emitindo ramos musculares para o bíceps femoral ou da coxa, e para os músculos semimembranoso e semitendinoso, que continua com um tronco calibroso, originando os nervos fibular lateralmente, medialmente o tibial e caudalmente o cutâneo sural plantar lateral.

  1. Comportamento reológico de méis de florada de silvestre Rheological behavior of honey from Serjania glabrata flowers

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    Alexandre J. de M. Queiroz

    2007-04-01

    Full Text Available Estudou-se o comportamento reológico de méis de Apis mellifera produzidos no estado do Piauí, no semi-árido brasileiro, elaborados com florada predominante de silvestre (Serjania glabrata. As amostras foram coletadas ao longo do ano 2000, a partir de coletas realizadas por apicultores, associações e cooperativas de apicultura da região abrangida pelos municípios de Picos, Itainópolis, Vera Mendes e Isaías Coelho. Após as coletas as amostras passaram pelos processos de centrifugação, filtragem e decantação. As medidas reológicas foram feitas nas temperaturas de 20 a 40 ºC utilizando-se um viscosímetro Brookfield, modelo RVT. As leituras de velocidade de rotação e torque foram transformadas em valores de taxa de deformação e tensão de cisalhamento. Os dados de taxa de deformação e tensão de cisalhamento foram ajustados pelas equações da Lei-da-Potência e de Herschel-Bulkley. As amostras apresentaram comportamento pseudoplástico. As equações se ajustaram adequadamente aos resultados experimentais de tensão de cisalhamento em função da taxa de deformação. O aumento de temperatura reduziu a viscosidade aparente em níveis próximos de 80%. Os valores de viscosidade aparente foram bem ajustados por uma equação do tipo Arrhenius.The rheological behavior of Apis mellifera honey produced in the State of Piauí, in the Brazilian semi-arid, obtained with predominance of Serjania glabrata flowers was studied. The samples were collected in the year 2000 by beekeepers and associations and cooperatives of beekeepers in the area covered by the municipal districts of Picos, Itainópolis, Vera Mendes and Isaías Coelho. The samples were centrifuged, filtered, decanted and the rheological measures at temperatures of 20 to 40 ºC using a Brookfield Viscometer RVT model were made. The readings of rotation speed and torque were transformed in shear rate and shear stress values. The data of shear rate and shear stress were fitted

  2. Memoria espacial en monos capuchinos de Argentina: un estudio observacional en vida silvestre / Spatial memory in capuchin monkeys (Argentina:a wildlife observational study

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    María P. Tujague

    2016-03-01

    Full Text Available La memoria espacial se considera un factor selectivo en la evolución de la inteligencia. Los estudios en animales permiten pensar que los procesos cognitivos descriptos en humanos comparten características con los de otras especies. Se estudió el comportamiento espacial vinculado a la búsqueda del alimento de los monos capuchinos y su capacidad de retener información sobre la ubicación y estado del mismo. Durante los seguimientos de tres grupos de Cebus nigritus silvestres se aplicó un diseño observacional cualitativo que evaluó las cantidades de fruta madura e inmadura presentes en los árboles frutales consumidos por los monos en sus desplazamientos diarios. Las visitas a los árboles de frutos indicaron que los monos retienen información particular sobre la ubicación y condición de cada árbol. Los resultados obtenidos son fundamentales para la comprensión del funcionamiento de los mecanismos cognitivos no mediados por el lenguaje, lo cual permite ampliar los conocimientos de la base biológica del comportamiento espacial no humano. Palabras clave: Cebus=Sapajus nigritus; árboles frutales; velocidad de acercamiento; visita Spatial memory is considered a selective factor in the evolution of intelligence. Animal studies lead us to believe that cognitive processes described in humans may have much in common with those of other species. We studied the foraging spatial behavior of capuchin monkeys and their ability to retain information about food location and quality. We analyzed whether individuals were able to integrate stored information about food sites and changes in the ripening of the resource. A qualitative observational design was used to monitor three groups of wild Cebus nigritus in their daily movements, evaluating the amounts of ripe and unripe fruit present in the fruiting trees consumed by monkeys. Visits to fruiting trees showed that monkeys remember specific information on the location and condition of each tree

  3. Estudio de la composición en ácidos grasos del aceite de las semillas en algunas plantas silvestres españolas

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    Vioque, J.

    1994-06-01

    Full Text Available The fatty acid composition of 34 species of plants from a variety of families that grow wild in the Iberian Peninsula was analysed. The aim to the survey was to indentify oils that contain a mix of fatty acids that from a qualitative or quantitative point of view have a commercial value. Because of the diverse taxonomic origin of the samples, the oil content between species was very variable, fluctuating between an average value of 3.4% in the Caryophyllaceae and 31.1% in Euphorbiaceae. Like the oil content, the fatty acid composition was quite variable, but, in general, the main fatty acids were palmitic, oleic, linoleic and linolenic acid, while in the Brassicaceae and in the Apiaceae erucic acid and petroselinic acid were the principal fatty acids respectively.

    Se ha estudiado la composición en ácidos grasos del aceite de las semillas en 34 plantas silvestres de la Península Ibérica. Se pretende la obtención de aceites con una composición en ácidos grasos que cualitativa o cuantitativamente puedan resultar de interés desde un punto de vista comercial. Debido al origen taxonómico tan diverso de las muestras, el contenido en aceite resulta muy variable, oscilando entre un valor medio del 3.4% en las Caryophyllaceae y el 31.1% de media en las Euphorbiaceae. Como el contenido en aceite, la composición en ácidos grasos es también bastante Variable, pero en general los principales ácidos grasos son palmítico, oleico, linoleico y linolénico, mientras que en las Brassicaceae domina el ácido erúcico y en las Apiaceae el ácido petroselínico.

  4. Evaluación de especies silvestres de Lycopersicon sp, como fuente de resistencia a Scrobipalpula absoluta (Meyrick y su intento de transferencia a Lycopersicon esculentum Mill

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    Parra S. Alvaro

    1989-06-01

    Full Text Available En condiciones de campo, se evaluaron 32 introducciones silvestres de Lycopersicon sp. y una de la especie cultivada, L. esculentum Mili, con el fin de determinar la resistencia a Scrobipalpula absoluta (Meyrick. Se utilizó el diseño experimental bloques completos al azar con 4 repeticiones. Como bordes se colocaron tres variedades comerciales susceptibles al insecto (Chonto Mata Verde, Napoli y Chonto Santa Cruz Kada con el fin de tener una fuente de infestación natural y permanente del cogollero dentro del experimento. Se evaluaron los caracteres número de cogollos afectados, número de hojas dañadas, número de ampollas e intensidad de daño. Todas las introducciones de L. hirsutum y L. peruvianum fueron altamente resistentes. Las introducciones 1406 y 1407 de L. pimpinellifolium y L. esculentum también fueron altamente resistentes. La introducción 1759 de L. esculentum var. ceraciforme fueron muy susceptibles. Las introducciones L. pimpinellifolium y L. hirsutum no tuvieron dificultad para hibridarse con L. esculentum var. Tropic. El cruzamiento L. esculentum x L. peruvianum no presento semilla híbrida y se requirió efectuar cultivo de embriones inmaduros in vitro utilizando el medio Murashige & Skoog pero sin lograrse resultados positivos.Thirty two (32 wild accessions of Lycopersicon sp. and one (1 cultivated accession of Lycopersicon esculentum Mill was evaluated to determinate the resistance to Scrobipulpa absoluta (Meyrick. The experiment was conducted with a complete random design and tour repetitions. Three (3 susceptibles cultivated accessions of L. esculentum (Chonto Mata Verde, Napoli and Chonto Santa Cruz Kada was sowed to have natural infestation of S. absoluta. Demage intensity, number of buds demaged, number of leaves demaged and number of "blister" in the leaves was evaluated. AII evaluated accessions of L. hirsutum and L. peruvianum showed height resistance. 1406 and 1407 accessions of L. pimpinellifolium also was

  5. Fenología de la floración en tomate cultivado y especies silvestres relacionadas Flowering phenology in cultivated tomato and related wild species

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    Edwin Fernando Restrepo Salazar

    2008-06-01

    Full Text Available En un diseño experimental de bloques completos al azar se comparó la fenología de la floración de doce accesiones silvestres de Solanum sección Lycopersicum con la del tomate cultivado tipo “chonto”. Los tratamientos se repitieron tres veces. Se evaluó la aparición de las 12 primeras inflorescencias y el tipo de inflorescencia. Las fenologías de las accesiones de la variedad glabratum de S. habrochaites (PI 134417, PI134418 y PI126449, las accesiones LA1624, LA2092 de la variedad typicum de S. habrochaites y la accesión LA 444-1 de S. peruvianum coincidieron con la del tomate “chonto”. No se presentaron diferencias significativas en la variable días a inicio de floración entre las accesiones de la variedad glabratum y el tomate cultivado. Las accesiones de la variedad glabratum de S. habrochaites y el tomate cultivado presentaron inflorescencias simples; mientras que las accesiones de la variedad typicum de S. habrochaites y de la especie S. peruvianum presentaron inflorescencias bifurcadas.The flowering phenology of twelve wild accesions of Solanum section Lycopersicum were compared with those of the cultivated tomato type of “chonto” by using the randomized complete block design. The treatments were repeated three times. The appearance initiate of the first twelve inflorescences and kind of inflorescence were evaluated. The phenologies of the accesions of the glabratum variety from S. habrochaites (PI 134417, PI134418 y PI126449, LA1624 y LA2092 of the typicum variety from S. habrochaites and LA 444-1 from S. peruvianum coincided with those of the “chonto” tomato. There were no significant differences between the glabratum variety accessions and cultivated tomato in the starting days of flowering variable. The accesions of the glabratum variety from S. habrochaites and the cultivated tomato presented simple inflorescences; while the accesions of the typicum variety from S. habrochaites and S. peruvianum specie showed

  6. BIODIVERSIDADE E INTERAÇÃO DE ECTOPARASITOS EM ROEDORES SILVESTRES Holochilus sciureus WAGNER, 1842 (RODENTIA: CRICETIDAE, CAPTURADOS NO ESTADO DO MARANHÃO, BRASIL

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    Guilherme Silva Miranda

    2017-03-01

    Full Text Available Objetivou-se realizar a identificação da ectoparasitofauna do roedor Holochilus sciureus de forma a descrever a dinâmica da inter-relação entre parasitos, o hospedeiro e o ambiente. Para tanto, esses animais foram capturados na cidade de São Bento, estado do Maranhão. Utilizaram-se pentes finos e pinças metálicas para a coleta dos ectoparasitos que foram conservados em etanol a 70% e identificados com auxílio de chaves taxonômicas. Calcularam-se parâmetros ecoepidemiológicos (abundância, prevalência, intensidade máxima e média de cada grupo identificado. A ocorrência de infestações mistas também foi investigada. Realizaram-se testes estatísticos de correlação de Pearson (p<0,05 entre as variáveis: abundância dos ectoparasitos, características biológicas do hospedeiro e índice pluviométrico da região. Foi obtido um total de 104 roedores e 5109 ectoparasitos, que foram identificados como: Prolistrophorus spp. (59,13%, Laelaps sp. (34,9%, Hoplopleura sp. (5,83% e larvas de Metacuterebra sp. (0,03%. Os valores dos parâmetros ecoepidemiológicos foram mais elevados para Prolistrophorus spp. As infestações triplas foram as mais frequentes (57,74%. Dentre as correlações realizadas, somente o comprimento total em roedores machos influenciou de maneira negativa e significativa a abundância de Laelaps sp. O ácaro Prolistrophorus spp., o piolho Hoplopleura e, larvas de Metacuterebra sp. constituem primeiros registros para o estado do Maranhão. Palavras-chave. artrópodes parasitos, mamífero silvestre, Maranhão.

  7. Genetic Relationships of Common Bean (Phaseolus vulgaris L. Race Chile with Wild Andean and Mesoamerican Germplasm Relaciones Genéticas entre el Germoplasma de Poroto (Phaseolus vulgaris L. Raza Chile y Silvestres Andinos y Mesoamericanos

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    Viviana Becerra V

    2011-03-01

    Full Text Available The Chilean common bean (Phaseolus vulgaris L. belongs to the cultivated race Chile and its origin is presumably Andean. The objective of this study was to identify the origin of a group of Chilean accessions based on their genetic relationship with wild material from the Mesoamerican and Andean common bean gene pool. To achieve this objective, universal primers of chloroplast DNA (cpDNA and mitochondrial DNA (mtDNA were used to detect polymorphism using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP. Thirty-two genotypes were analyzed, including wild material from Mexico, Ecuador, Peru, Bolivia, and Argentina, as well as Chilean cultivated genotypes belonging to endemic Chilean accession types (Tórtola, Coscorrón, and Cuyano and naturalized commercial lines (Frutilla, Bayo, Manteca, and Blanco grande. Results showed a low level of polymorphism for cpDNA (23% and mtDNA (24% in wild and cultivated Chilean common bean accessions. Some universal primers and restriction enzyme combinations were more efficient than others in detecting polymorphism. The Chilean materials were closely related to wild accessions collected in Argentina, Bolivia, and Peru indicating their Andean origin. The wild accessions from Ecuador were located in a intermediate position between the Mesoamerican and Andean accessions.El poroto chileno (Phaseolus vulgaris L. pertenece a la raza Chile, cuyo origen es desconocido y presumiblemente andino. El objetivo del trabajo fue identificar el origen de un grupo de genotipos chilenos basado en sus relaciones genéticas con material silvestre perteneciente a los acervos genéticos mesoamericano y andino. Para lograr este objetivo se usaron partidores universales de ADNcp y ADNmt con la metodología de la Reacción en Cadena de la Polimerasa-Fragmentos de Restricción Polimórficos. (PCR-RFLP. Se analizó un total de 32 genotipos de P. vulgaris, los cuales incluyeron materiales silvestres de M

  8. RNA-Seq analysis reveals genes associated with resistance to Taura syndrome virus (TSV) in the Pacific white shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Sookruksawong, Suchonma; Sun, Fanyue; Liu, Zhanjiang; Tassanakajon, Anchalee

    2013-12-01

    Outbreak of Taura syndrome virus (TSV) is one of the major pathogens of the Pacific white shrimp Litopenaeus vannamei. Although selective breeding for improvement of TSV resistance in L. vannamei has been successfully developed and has led to a great benefit to the shrimp farming industry worldwide. The molecular mechanisms underlying the viral resistance in shrimp remain largely unknown. In the present study, we conducted the first transcriptomic profiling of host responses in hemolymph and hemocytes in order to identify the differentially expressed genes associated with resistance to TSV in L. vannamei. High-throughput RNA-Seq was employed, obtaining 193.6 and 171.2 million high-quality Illumina reads from TSV-resistant and susceptible L. vannamei lines respectively. A total of 61,937 contigs were generated with an average length of 546.26 bp. BLASTX-based gene annotation (E-value vannamei. Copyright © 2013 Elsevier Ltd. All rights reserved.

  9. Effect of replacing fish meal with extruded soybean meal on growth, feed utilization and apparent nutrient digestibility of juvenile white shrimp ( Litopenaeus vannamei)

    Science.gov (United States)

    Yang, Qihui; Tan, Beiping; Dong, Xiaohui; Chi, Shuyan; Liu, Hongyu

    2015-10-01

    Extruded soybean meal (ESBM) was evaluated as a protein source for partial replacement of fish meal (FM) in diets of juvenile Litopenaeus vannamei. In the control diet (Diet 1), FM protein was replaced with increasing dietary levels of ESBM (4.28%, 8.40%, 12.62%, 16.82%, and 25.26%) at 10%, 20%, 30%, 40%, and 60% levels (Diets 2 to 6, respectively). An eight-week feeding trial was conducted on 720 juvenile shrimp (0.67 g ± 0.01 g mean initial weight), and nutrient digestibility of the six diets was determined. ESBM could replace 20% of FM without causing a significant reduction in growth of shrimp, but other dietary treatments strongly affected whole body composition. Crude protein content of the whole body fed Diet 6 was significantly lower than that fed Diet 2 ( P vannamei.

  10. Genetic diversity within and between broodstocks of the white shrimp Litopenaeus vannamei (Boone, 1931 (Decapoda, Penaeidae and its implication for the gene pool conservation

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    PD. Freitas

    Full Text Available Genetic variation within and between fifteen closed broodstock lines of the Pacific white shrimp Litopenaeus vannamei, reared at different hatcheries in the Brazilian coast, was assessed by RAPD analysis. Fifty two polymorphic loci were identified when a set of five decamer primers was used in PCR. The genetic diversity analysis within lines evidenced genetic variation loss probably related to bottleneck effects and inbreeding. In addition, the genetic divergence values between the different samples appear to reflect the initial founder composition of such stocks, in some cases, sharing a common origin, suggesting a putative importance of interbreeding for the establishment of genetic improvement programs for these broodstocks. The genetic variation monitoring appears to be helpful to the gene pool conservation of this aquaculture species, mainly if considered its exotic status in Brazil and the current impossibility of new introduction of wild individuals.

  11. Does damming of the Colorado River affect the nursery area of blue shrimp Litopenaeus stylirostris (Decapoda: Penaeidae in the Upper Gulf of California?

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    Eugenio Alberto Aragón-Noriega

    2000-12-01

    Full Text Available After damming the Colorado River the freshwater flow was reduced to 1 % of its virgin flow to the Upper Gulf of California (UGC. The ecological effects need to be properly documented. The UGC is the nursery area for Litopenaeus stylirostris, the most profitable fishery in the zone. In order to know the relative abundance of L. stylirostris postlarval stage we conducted a sampled survey every 14 days in 1993, 1994 and 1997, plus an intensive sampling during a complete tide cycle in July 1995 and 1996. We did 10 min trawls each hour during the flood tide. Relative abundance of postlarvae was higher (pEl represamiento del Río Colorado ha ocasionado que el flujo de agua dulce sobre el Alto Golfo de California (AGC se haya reducido hasta el 1 % del flujo original. Se ha documentado el efecto de la reducción de agua dulce sobre las condiciones hidrográficas del AGC, pero las repercusiones ecológicas no se han descrito apropiadamente. El AGC ha sido área de crianza para especies comerciales como el camarón Litopenaeus stylirostris. Se hicieron recolectas de postlarvas de L. stylirostris en el AGC durante cinco años consecutivos. Los muestreos fueron catorcenalmente en los años de 1993, 1994 y 1997 y se realizó una recolecta diaria durante 15 días consecutivos en los años 1995 y 1996. Para ello se arrastró una red de plancton de 505 µ durante 10 min cada hora durante el flujo de marea. La abundancia relativa de las postlarvas de camarón en esta zona viaria considerablemente en años cuando el flujo de agua dulce incrementa. La abundancia es mayor hasta en un 200 % (p < 0.05 cuando existe descarga de agua dulce al AGC.

  12. Evaluación del gluten de maíz como una fuente reemplazante de la harina de pescado en dietas para el camarón juvenil litopenaeus vannamei

    OpenAIRE

    Molina, César

    2004-01-01

    Evaluación del gluten de maíz como una fuente reemplazante de la harina de pescado en dietas para el camarón juvenil Litopenaeus vannamei En alimentos para especies acuáticas comerciales, la harina de pescado es una de las fuentes de proteína marina más ampliamente usada, por su alto valor nutricional y palatabilidad.

  13. Polyculture of white shrimp, Litopenaeus vannamei and milkfish, Chanos chanos as a strategy for efficient utilization of natural food production in ponds

    OpenAIRE

    Bessie Joy G. Elle; Christopher M. A. Caipang; Cecilia J. Jaspe

    2011-01-01

    Both shrimps and milkfish are important aquaculture species in the Philippines. In the presentstudy, we described a modified extensive polyculture method for white shrimp, Litopenaeus vannameiand miklkfish, Chanos chanos in ponds during the wet months. This particular culture system uses two ormore species in a particular production area with the purpose of maximizing the utilization of theavailable natural food present in the system. Two 1-hectare earthen ponds were prepared and addedwith or...

  14. Diversidad genética y relaciones de parentesco de las poblaciones silvestres y cultivadas de pejibaye (Bactris gasipaes, Palmae, utilizando marcadores microsatelitales

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    José Alfredo Hernández Ugalde

    2008-03-01

    Full Text Available Se evaluó la diversidad genética en cuatro microsatélites de ADN de pejibaye (Bactris gasipaes Kunth para relacionarlos con su evolución y domesticación. Se analizaron 258 muestras procedentes de siete poblaciones silvestres y once razas cultivadas. Todos los loci eran polimórficos y se identificaron 50 alelos en total. La diversidad genética fue alta (0.67. Todas las poblaciones reunidas obtuvieron una alta diferenciación genética (Fst=0.16, pero cuando se separaron en poblaciones occidentales y orientales fue menor (Fst=0.13 para ambas. El flujo genético presente en las poblaciones occidentales fue mayor (Nm=1.71 que en las orientales (Nm=1.62. Por otra parte, se encontró que las razas de Putumayo, Yurimaguas, Vaupés, Tucurrique, y Guatuso aparentemente han sido sometida a una intensa selección humana. Además, la existencia de poblaciones híbridas es el resultado del intercambio entre pueblos del neotrópico e introgresiones con poblaciones silvestres y cultivadas. Se estimó la distancia genética Dm para generar un dendograma por el método del vecino más cercano. Definimos tres grupos de poblaciones: Maracaibo (B. caribaea, B. macana var veragua y B. macana var arapuey, Amazonía Oriental (Tembe, Pará y Acre y el grupo compuesto por dos subgrupos, Occidental (Azuero, Chontilla, Tuira, Cauca, Tucurrique y Guatuso y Alto Amazonas (B. dahlgreniana, Puerto Ayacucho, Solimões, Vaupés y Putumayo. La relación genética coincide con la hipótesis de que la palmera del pejibaye ha sido domesticada independientemente por lo menos en tres regiones.Genetic diversity and kin relationships among wild and cultivated populations of the pejibaye palm (Bactris gasipaes, Palmae using microsatellite markers. The genetic diversity of the peach palm (Pejibaye, Bactris gasipaes Kunth was evaluated using four nuclear DNA microsatellites in an effort to elucidate the evolution and domestication of this crop. A total of 258 samples from seven

  15. Effect synergistic of the radiation and of the refrigeration in the conservation of the white shrimp (Litopenaeus vannamei);Efeito sinergistico da radiacao gama e de refrigeracao na conservacao do camarao-branco-do-pacifico (Litopenaeus vannamei)

    Energy Technology Data Exchange (ETDEWEB)

    Guimaraes-Lopes, Tatiana Giselle

    2006-07-01

    Taking into account shrimp susceptibility to autolise and bacterial decomposition, this study aimed to evaluate the different gamma irradiation doses influence over meat quality related to microbiological, physical-chemical, nutritional and sensorial aspects, during cold storage of the white shrimp (Litopenaeus vannamei). Samples were taken from fresh shrimps without cephalothoraxes, settled into polystyrene trays, packed with flexible plastic film and submitted to different doses of gamma irradiation (1.0 and 3.5 kGy). Samples were kept under refrigeration (5 deg C) and analyzed at the 1st, 7th, 14th and 21st day of storage. Microbiological analyzes were realized (Coagulase-positive Staphylococcus, Salmonella ssp, coliforms, psychotropic and mesophilic population), and physical-chemical and tests (Total volatile bases (TVNB), pH, non proteic nitrogen (NNP), TBARS analysis - reactive substances to thiobarbituric acid (TBA), trimetil amine (TMA) cholesterol tenor and proximate composition). Instrumental color test were realized and hedonic test was taken to determine cooked products sensorial quality during storing period. It was noticed that the irradiation process reduced bacterial population and during storage all the irradiated samples were under the patterns established by the Brazilian legislation (Brazil, 2005a). Non-irradiated samples were inappropriate for consumption after one week of storage, since they were over Staphylococcus established limits. The pH values ranged from 6.05 to 6.39; 19.47 to 80.84 mg.100g-1 for TVNB; 2.61 to 12.85 mg.100g-1 for TMA-N; 0.22 a 1.20 mg of malonaldeide.1000g{sup -1} for TBA and 53.09 to 134.81 mg.100g-1 for cholesterol concentrations. Irradiated samples kept more stability during storage period when compared to non-irradiated samples, regarding to pH, TVNB and TMA levels. Samples irradiation resulted into cholesterol and TBA values reduction at different evaluated moments. No significant differences (p <= 0.05) were found

  16. Avaliação dos parâmetros físico-químicos e análise por componentes principais de méis silvestres produzidos e comercializados no Estado do Rio de Janeiro, Brasil

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    Fábio Cerdeira LIRIO ;Mariana dos Santos BELLO ; Mirian Ribeiro Leite MOURA

    2015-01-01

    Full Text Available O objetivo do trabalho foi avaliar os parâmetros físico-químicos de méis silvestres comercializados no Rio de Janeiro. Foram analisadas 24 amostras, no período entre 2007 e 2008. A análise por componentes principais foi executada para aprimorar os resultados obtidos na caracterização físico-química dos méis silvestres. Os resultados da avaliação físicoquímica mostraram que 12 amostras (50 % estavam em conformidade com a legislação vigente, 10 amostras (42 % foram reprovadas em um parâmetro físico-químico e 2 amostras (8 % foram reprovadas em 2 ou mais parâmetros de qualidade. A análise por componentes principais revelou que três componentes principais apresentaram 84,8 % da variância total dos dados. Além disso, 75 % dos parâmetros físico-químicos foram adequadamente apresentados pelos três componentes principais. Contudo, não foi possível observar o agrupamento eficiente das amostras, por regionalização ou prazo de validade, devido ao comportamento heterogêneo dos parâmetros físico-químicos dos méis silvestres. Portanto, a análise de componentes principais não permitiu a classificação dos méis silvestres. Além disso, a análise físico-química mostrou que as medidas de controle da qualidade devem ser aprimoradas com o objetivo de garantir a qualidade dos méis silvestres produzidos e comercializados no Rio de Janeiro.

  17. Effects of dietary mannan oligosaccharide on growth performance, gut morphology and stress tolerance of juvenile Pacific white shrimp, Litopenaeus vannamei.

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    Zhang, Jian; Liu, Yongjian; Tian, Lixia; Yang, Huijun; Liang, Guiying; Xu, Donghui

    2012-10-01

    An 8-week feeding trial was conducted to investigate the effects of dietary mannan oligosaccharide (MOS) on growth performance, gut morphology, and NH(3) stress tolerance of Pacific white shrimp Litopenaeus vannamei. Juvenile Pacific white shrimp (1080 individuals with initial weight of 2.52 ± 0.01 g) were fed either control diet without MOS or one of five dietary MOS (1.0, 2.0, 4.0, 6.0 and 8.0 g kg(-1)) diets. After the 8-week feeding trial, growth parameters, immune parameters, intestinal microvilli length and resistance against NH(3) stress were assessed. Weight gain (WG) and specific growth rate (SGR) were significantly higher (P shrimp fed 2.0, 4.0, 6.0 and 8.0 g kg(-1) MOS-supplemented diets than shrimp fed control diet. WG and SGR of shrimp fed 2.0 g kg(-1) MOS-supplemented diet was the highest (P shrimp was generally similar (P > 0.05) in all experimental groups. Compared with control group, TEM analysis revealed that 2.0, 4.0, 6.0 and 8.0 g kg(-1) MOS supplementation could significantly increase (P shrimp at the ultrastructural level. After NH(3) stress for 24 h, SR of shrimp fed 2.0, 4.0, 6.0 and 8.0 g kg(-1) MOS-supplemented diets was significantly higher (P shrimp fed control diet. Phenoloxidase (PO) activity of 4.0 g kg(-1) MOS-supplemented group was significantly higher (P < 0.05) than that of control group under normal conditions and NH(3) stress. PO activity significantly decreased (P < 0.05) under NH(3) stress than under normal conditions. Superoxide dismutase (SOD) activity of 4.0, 6.0 and 8.0 g kg(-1) MOS-supplemented groups was significantly higher (P < 0.05) than that of control group under normal conditions. After NH(3) stress for 24 h, SOD activity of all experimental groups also significantly decreased (P < 0.05) compared to normal conditions. These results clearly indicated that dietary MOS could improve growth performance and increase the resistance against NH(3) stress in L. vannamei, and the 2.0-4.0

  18. Las abejas silvestres de Colombia: Por qué y cómo conservarlas The Colombian wild bees: Why and how to preserve them

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    González Víctor Hugo

    2000-06-01

    Full Text Available Las abejas silvestres de Colombia, al igual que las del resto del mundo, están sufriendo los rigores de las actividades desarrolladas por el hombre y así se catalogan como organismos en peligro. El conocimiento actual de las abejas en nuestro país, como muchos otros insectos, todavía se encuentra en una fase muy incipiente; es fragmentado, local y carece de una síntesis. Tan sólo se conoce racionalmente 5% de las abejas del país, especialmente las especies corbiculadas de la familia Apidae. Muy poco trabajo taxonómico ha sido realizado por investigadores colombianos y prácticamente no existen trabajos por autores extranjeros enfocados en la fauna colombiana. La carencia de especialistas, las dificultades para el envío de material al exterior y la falta de recursos han dificultado la tarea. Sin embargo, la alta riqueza de especies que creemos se encuentra en el país representa una razón más para avanzaren el conocimiento y entendimiento de la biodiversidad de éste grupo. En esta revisión se analizan las principales causas que ponen en riesgo las poblaciones y especies de la apifauna nativa: deforestación, pastoreo, la abeja africanizada y la explotación irracional de las abejas, entre otras. Se proponen algunas acciones a ser emprendidas conjuntamente con centros académicos, agricultores, campesinos, indígenas y la sociedad en general que permitan proteger las especies, teniendo en cuenta que median-te la polinización, se tornan en eslabones esenciales dentro de los ecosistemas permitiendo la conservación de muchas especies vegetales y otras comunidades.The Colombian wild bees, as well as those in the rest of the whole world are undergoing the rigor of human activities and so are considered to be under threat of extinction. The current knowledge about Colombian bees, like other insects, is still in its beginnings, is fragmentated and a synthesis is desirable. Only 5% of the Colombian bees are well known, especially the

  19. Flowering phenology in cultivated tomato and related wild species Fenología de la floración en tomate cultivado y especies silvestres relacionadas

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    Lobo Arias Mario

    2008-06-01

    Full Text Available The flowering phenology of twelve wild accesions of Solanum section Lycopersicum were compared with those of the cultivated tomato type of “chonto” by using the randomized complete block design. The treatments were repeated three times. The appearance initiate of the first twelve inflorescences and kind of inflorescence were evaluated. The phenologies of the accesions of the glabratum variety from S. habrochaites (PI 134417, PI134418 y PI126449, LA1624 y LA2092 of the typicum variety from S. habrochaites and LA 444–1 from S. peruvianum coincided with those of the “chonto” tomato. There were no significant differences between the glabratum variety accessions and cultivated tomato in the starting days of flowering variable. The accesions of the glabratum variety from S. habrochaites and the cultivated tomato presented simple inflorescences; while the accesions of the typicum variety from S. habrochaites and S. peruvianum specie showed compound inflorescences.Key words: Solanum lycopersicum; S. habrochaites; S. peruvianum; kind of inflorescence.En un diseño experimental de bloques completos al azar se comparó la fenología de la floración de doce accesiones silvestres de Solanum sección Lycopersicum con la del tomate cultivado tipo “chonto”. Los tratamientos se repitieron tres veces. Se evaluó la aparición de las 12 primeras inflorescencias y el tipo de inflorescencia. Las fenologías de las accesiones de la variedad glabratum de S. habrochaites (PI 134417, PI134418 y PI126449, las accesiones LA

  20. RESISTENCIA AL PERFORADOR DEL FRUTO DEL TOMATE DERIVADA DE ESPECIES SILVESTRES DE Solanum spp. RESISTANCE TO TOMATO FRUIT BORER DERIVED FROM WILD SPECIES OF Solanum spp.

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    Franco Alirio Vallejo Cabrera

    2008-06-01

    Full Text Available Se evaluó la resistencia al pasador del fruto Neoleucinodes elegantalis en doce introducciones silvestres de Solanum sección Lycopersicum. Las introducciones PI 134417, PI134418 y PI 126449 Solanum habrochaites var. glabratum, las introducciones LA 1624 y LA2092 de S. habrochaites var. typicum y la introducción LA 444-1 de S. peruvianum, presentaron alta resistencia al pasador del fruto de tomate N. elegantalis. Se realizó la introgresión genética de la resistencia al pasador del fruto presente en la introducción PI 134418 de S. habrochaites var. glabratum hacia el cultivar Unapal - Maravilla de S. lycopersicum. Se encontró asociación positiva altamente significativa entre el daño causado por el insecto plaga y el peso del fruto; a medida que se recupera el peso del fruto del cultivar Unapal - Maravilla (padre recurrente, por sucesivos retrocruzamientos, se disminuye la resistencia al insecto plaga. Los tricomas y el número de frutos por racimo no afectaron la infestación y el daño de los frutos por parte del insecto; mientras que el peso de fruto si tuvo efecto importante ya que tiende a producir mayor daño a medida que se incrementa el peso de fruto. El método del retrocruzamiento fue efectivo para romper la asociación entre el peso de fruto y la resistencia al insecto. Se obtuvieron plantas recombinantes RC2 con resistencia al pasador del fruto y pesos de fruto entre 45,1 y 68,6g.Twelve wild introductions of Solanum section Lycopersicum were evaluated to determine their resistance to tomato fruit borer Neoleucinodes elegantalis. The introductions PI 134417, PI134418 and PI 126449 of Solanum habrochaites var. glabratum, the introduction LA 1624 and LA2092 of S. habrochaites var. typicum, and the introduction LA 444-1 of S. peruvianum, presented high resistance to the tomato fruit borer of tomato N. elegantalis. Genetic introgression of resistance to tomato fruit borer in the introduction PI 134418 S. habrochaites var. glabratum

  1. Procedencia y uso de madera de pino silvestre y pino laricio en edificios históricos de Castilla y Andalucía

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    Rodríguez Trobajo, Eduardo

    2008-12-01

    Full Text Available The productive cycle of Scots pine (Pinus sylvestris L. and black pine (Pinus nigra Arn. within the medieval Castilian and Andalusian carpentry is here analysed. New criteria to identify both timbers and its dendrochronological date are shown as previous facts to determine their geographical origin. Historical names of these species and other names, such as alerce, with a vague allocation are also studied. Main historical areas of resource and river ways of Tajo and Guadalquivir used to transport the wood (black pine to interior cities (Sevilla, Toledo, Madrid … are thus identified. On the other hand, wood’s diversification and its selective use is analysed regarding its resistance value. Availability also determines that a sort of wood becomes fossil-guide (especie-guía, which is proposed as chronological indicator for several periods and constructive contexts. Some timber remains dating to the first millennium are studied in detail. They belong to the Mosque of Cordoba and four early medieval churches sited in the Duero valley (La Nave, Baños, Quintanilla and Barriosuso. The empiric dating and analysis of this material offer a post quem chronology for the building of these churches and new facts about the distribution of theses wood’s species in the north-western Iberian peninsula.Se realiza un recorrido a través del ciclo constructivo de las maderas de pino silvestre (Pinus sylvestris L. y pino laricio (Pinus nigra Arn. en el ámbito de la carpintería medieval de Castilla y Andalucía. Nuevos criterios para diferenciar las dos especies de madera y su datación dendrocronológica, son aportados como datos previos para determinar el origen geográfico del material. Son objeto de discusión los nombres históricos de estas especies y otras voces, como alerce, que tienen una imprecisa asignación. Se identifican así las principales áreas históricas de aprovechamiento y las vías fluviales del Tajo y Guadalquivir utilizadas para el

  2. Characterization of rust, early and late leaf spot resistance in wild and cultivated peanut germplasm Caracterização da resistência à ferrugem, mancha preta e mancha castanha em germoplasma silvestre e cultivado de amendoim

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    Alessandra Pereira Fávero

    2009-02-01

    Full Text Available Groundnut (Arachis hypogaea has an AB genome and is one of the most important oil crops in the world. The main constraints of crop management in Brazil are fungal diseases. Several species of the genus Arachis are resistant to pests and diseases. The objective of our experiments was to identify wild species belonging to the taxonomic section Arachis with either A or B (or " non-A" genomes that are resistant to early leaf spot (Cercospora arachidicola, late leaf spot (Cercosporidium personatum and rust (Puccinia arachidis. For the identification of genotypes resistant to fungal diseases, bioassays with detached leaves were done in laboratory conditions, with artificial inoculation, a controlled temperature of 25ºC and a photoperiod of 10 h light/14 h dark, for 20-42 days, depending on the fungi species. Most of the accessions of wild species were more resistant than accessions of A. hypogaea for one, two or all three fungi species studied. Arachis monticola, considered to be a possible tetraploid ancestor or a derivative of A. hypogaea, was also more susceptible to Cercosporidium personatum and Puccinia arachidis, as compared to most of the wild species. Therefore, wild germplasm accessions of both genome types are available to be used for the introgression of resistance genes against three fungal diseases of peanut.O amendoim (Arachis hypogaea possui genoma AB e é uma das mais importantes culturas oleaginosas em todo o mundo. Os principais problemas da cultura no Brasil são as doenças fúngicas. Várias espécies do gênero Arachis são resistentes a pragas e doenças. Este trabalho visou a identificar espécies silvestres pertencentes à seção Arachis associadas aos genomas A ou B (ou " não-A" do amendoim que são resistentes à mancha castanha (Cercospora arachidicola, mancha preta (Cercosporidium personatum e ferrugem (Puccinia arachidis. Para a identificação de genótipos resistentes a doenças fúngicas, bioensaios utilizando

  3. Efecto tóxico de DDT y endosulfan en postlarvas de camarón blanco, Litopenaeus vannamei (Decapoda:Penaeidaede Chiapas,México

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    Vicente Castro-Castro

    2005-06-01

    Full Text Available Con el fin de conocer la toxicidad del DDT y endosulfan sobre postlarvas de camarón blanco (Litopenaeus vannaei, se realizaron pruebas de toxicidad aguda en condiciones de laboratorio por 168 h, con temperatura de 29 ± 1 °C, salinidad de 3 ± 1 ‰ y pH en 8 ± 1.Se calculó la concentración letal media (LC50 , la LC50 "incipiente", los tiempos medios de muerte (LT50 , la Máxima Concentración Aceptable del Tóxico (MACT y el "Nivel de Seguridad" (LS; así mismo, en los organismos sobrevivientes se determinó la concentración a la que el crecimiento de los organismos se reduce en un 5 y 50% (CE5 y CE50 . Se evaluaron además las alteraciones en el consumo de oxígeno. El DDT fue 3 veces más tóxico que el endosulfan; sin embargo, los organismos resultaron ser muy sensibles a ambos compuestos. La tasa de crecimiento de las postlarvas disminuyó en un 80 y 50% para el DDT y endosulfan respectivamente. La baja resistencia de las postlarvas al DDT y endosulfan, y las concentraciones de estos compuestos en la laguna, sugieren que si se diera un ingreso adicional de estos plaguicidas al sistema, es muy probable un potencial impacto en la producción de camarón del sistemaToxic efect of DDT and endosulfan in white shrimp postlarvae Litopenaeus vannamei (Decapoda: Penaeidae from Chiapas, Mexico .We analized acute toxicity in white shrimp (Litopenaeus vannamei postlarvae exposed to two chlorinated pesticides, DDT and endosulfan, under laboratory conditions during 168 hours, with controlled temperature (29 ± 1°C, salinity (3 ± 1 ‰ and pH (8 ± 1. Median lethal concentrations (LC50 , "incipient" LC50, median lethal time (LT50 the "maximum acceptable concentration of the toxic compound" (MACT and "the safety level" (SL were determined. The concentration of the compounds at which organism growth was reduced by 5 and 50% (EC5 and EC50 , as well as changes in oxygen consumption patterns were determined in the surviving postlarvae.They were very

  4. Experimental infection with different bacterial strains in larvae and juvenile Litopenaeus vannamei reared in Santa Catarina State, Brazil - doi: 10.4025/actascibiolsci.v32i3.5471 Experimental infection with different bacterial strains in larvae and juvenile Litopenaeus vannamei reared in Santa Catarina State, Brazil - doi: 10.4025/actascibiolsci.v32i3.5471

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    Adolfo Jatoba

    2010-09-01

    Full Text Available This study evaluated the pathogenic characteristics of bacteria isolated from Litopenaeus vannamei during an outbreak at the Laboratory of Marine Shrimp, UFSC, Santa Catarina State, Brazil. Their virulence potential in larvae and juvenile shrimp and the effects on the total haemocyte count, phenoloxidase activity and serum agglutinate titre were examined after experimental infection. Bacterial strains were isolated from larvae and adult shrimps, identified by the AP120E biochemical system as: two strains of Vibrio alginolyticus, three of Aeromonas salmonicida and one of Pasteurella multocida sp. and Pasteurella sp. All the bacterial strains isolated in this study caused mortality in shrimp. One strain of V. alginolyticus was responsible for 97.3 and 88.7% mortality in larvae and juvenil shrimps, respectively. The shrimp immunological system was influenced by experimental infection with V. alginolyticus. Decrease in the total haemocyte count and increase in the phenoloxidase activity and the serum agglutinate titre (p V. alginolyticus isolated from larvae and juvenile reared marine shrimp.This study evaluated the pathogenic characteristics of bacteria isolated from Litopenaeus vannamei during an outbreak at the Laboratory of Marine Shrimp, UFSC, Santa Catarina State, Brazil. Their virulence potential in larvae and juvenile shrimp and the effects on the total haemocyte count, phenoloxidase activity and serum agglutinate titre were examined after experimental infection. Bacterial strains were isolated from larvae and adult shrimps, identified by the AP120E biochemical system as: two strains of Vibrio alginolyticus, three of Aeromonas salmonicida and one of Pasteurella multocida sp. and Pasteurella sp. All the bacterial strains isolated in this study caused mortality in shrimp. One strain of V. alginolyticus was responsible for 97.3 and 88.7% mortality in larvae and juvenil shrimps, respectively. The shrimp immunological system was influenced by

  5. Variacion estacional de una población silvestre de Rhodnius Pallescens Barber 1932 (Heteroptera: triatominae en la costa caribe colombiana

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    1998-01-01

    Full Text Available VARIATION SAISONNIERE D’UNE POPULATION SAUVAGE DE RHODNIUS PALLESCENS BARBER, 1932 (HETEROPTERA:TRIATOMINAE SUR LA COTE CARAÏBE COLOMBIENNE. Dans le cadre du programme franco-colombien “Écologie des triatomes sauvages (Triatominae du département de Sucre (Colombie et évaluation de l’efficacité d’une biopréparation insecticide à partir du champignon entomopathogène Beauveria bassiana (Hyphomycetes” (1994-1996, les auteurs ont étudié la dynamique de population de l’insecte hématophage Rhodnius pallescens dans une palmeraie de l’espèce Attalea butyracea (Arecaceae dans le municipio (cf. nota 1 de San Onofre (côte caraïbe colombienne. Au cours de la saison sèche et de la saison des pluies, un total de 71 palmiers ont été disséqués et 1 356 insectes capturés (163 adultes et 1 196 individus de tous les stades larvaires. 86% des palmiers étaient infestés (83% pendant la saison sèche et 91% pendant la saison des pluies la moyenne d’insectes retrouvés par palmier était de 19,5 (23,6 lors de la saison sèche et 17,6 pendant la saison des pluies. L’analyse des données permet de conclure que l'élément saison n’influe pas sur les variations des densités des insectes mais agit sur les compositions d’âge des populations de R. pallescens et que, même si l’évaluation du bioinsecticide peut se faire pendant toute l’année, il est recommandé de l’effectuer en novembre, au début de la saison des pluies. En el marco del programa colombo-francés “Ecología de triatominos silvestres (Triatominae del Departamento de Sucre (Colombia y evaluación de la eficacia de una biopreparación insecticida en base al hongo entomopatógeno Beauveria bassiana (Hyphomycetes” (1994-1996, los autores estudiaron la dinámica poblacional del insecto hematófago Rhodnius pallescens en un palmeral de la especie Attalea butyracea (Arecaceae en el municipio (1 de San Onofre (costa caribe colombiana. Se realizaron un total de 71 disecciones

  6. Extraction, partial characterization and evaluation of in vitro digestibility of the protein associated with the exoskeleton of white shrimp (Litopenaeus vannamei

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    Escobedo-Lozano, A.Y.

    2014-01-01

    Full Text Available The need of new food sources to satisfy human requirements forces researchers to study any possible alternative supplies. Therefore this study aimed to explore the quality and digestibility evaluation of the protein fractions obtained as a by-product of processing the shrimp exoskeleton Litopenaeus vannamei. Shrimp exoskeletons were washed, they were dried, mincedand they were brought under acid hydrolysis and alkaline hydrolysis; released proteins were then precipitated and characterized. 496 g of demineralized exoskeleton were obtained from 1 kg of shrimp shell; out of these, 376 g corresponded to chitin and 120 g corresponded to protein Total protein content was 33.80 ± 0.34 %. The digestible protein fractions were 26.7 g and 92.1 g were insoluble proteins. Of this latter fraction, the largest proportion belonged to the scleroprotein type. The amino acid profile analysis from the recovered proteins indicated that there were present nine amino acids out of the ten essential ones, being leucine the one with the highest proportion. In vitro digestibility was shown to be up to an 83.7 %. Therefore, it was possible to conclude that the recovery of digestibleproteins from shrimp exoskeleton may be useful in diet formulation.

  7. Contamination assessments of surface water in coastal lagoon (Maluan Bay, China) incorporating biomarker responses and bioaccumulation in hepatopancreas of exposed shrimp (Litopenaeus vannamei)--an integrative approach.

    Science.gov (United States)

    Wang, Zaosheng; Dong, Xiaoxia; Zhou, Shilei; Yan, Changzhou; Yan, Yijun; Chi, Qiaoqiao

    2014-01-01

    Maluan Bay, characterized by various degrees of anthropogenic contamination, is considered as one of the most industrialized and urbanized coastal lagoon in China, where large amounts of metal contaminants in surface water and biota were detected in previous studies. However, no clear discriminating power among sampling sites could be made only through comparisons between contaminant levels and Environmental Quality Standards and especially biological-based monitoring integrating biomarkers and bioaccumulation of exposure are scarce. For this purpose, antioxidants enzymes (superoxide dismutase, catalase, and glutathione peroxidase) and glutathione-S-transferase were assessed using the hepatopancreas of shrimp Litopenaeus vannamei after 7 days laboratory exposure under controlled conditions to characterize the effects of polluted waters to shrimps. The metal concentrations of sampled water and bioaccumulation in hepatopancreatic tissues were also analyzed, and data were linked to biomarkers' responses by multivariate (principal component analysis-factor) analysis. A representation of estimated factor scores was performed to confirm the factor descriptions classifying the pollution status and characterizing the studied sites, which pointed out the impact of multiple sources of contaminants to the water quality and provided further evidences to the existence of clear pollution and toxicological gradients in critical areas. The results of the present investigation underlined that the integrated approach could be a powerful tool for the identification of causal toxic contaminants in complex mixtures and the assessment of human-induced environmental quality of the system in coastal zones.

  8. Hemolymph osmolality and cation concentrations in Litopenaeus vannamei during exposure to artificial sea salt or a mixed-ion solution: relationship to potassium flux.

    Science.gov (United States)

    Sowers, A D; Young, S P; Grosell, M; Browdy, C L; Tomasso, J R

    2006-10-01

    Interest in culturing the Pacific white shrimp Litopenaeus vannamei in low-salinity and brackish-well waters has led to questions about the ability of this species to osmo- and ionoregulate in environments containing low concentrations of ions and in environments with ionic ratios that differ from those found in sea water. After seven days, hemolymph osmolality and potassium, sodium and calcium values were all significantly affected by salinity (as artificial sea salt) with values decreasing with decreasing salinity. These decreases were small, however, relative to decreases in salinity, indicating iono- and osmoregulation with adjustment for gradients. The hemolymph osmolality and sodium and calcium concentrations in shrimp exposed to either 2 g/L artificial sea salt or 2 g/L mixed-ion solution (a mixture of sodium, potassium, calcium, and magnesium chlorides that approximate the concentrations and ratios of these cations found in 2 g/L dilute seawater) did not differ significantly. However, hemolymph potassium levels were significantly lower in shrimp held in the mixed-ion environment. Potassium influx rates were similar in shrimp held in either artificial sea salt or mixed ions. The results of this study indicate that salinity affects hemolymph-cation concentrations and osmolality. Further, differential potassium-influx rates do not appear to be the basis for low hemolymph potassium levels observed in shrimp held in mixed-ion environments.

  9. Dynamics of vitellogenin and vitellogenesis-inhibiting hormone levels in adult and subadult whiteleg shrimp, Litopenaeus vannamei: relation to molting and eyestalk ablation.

    Science.gov (United States)

    Kang, Bong Jung; Okutsu, Tomoyuki; Tsutsui, Naoaki; Shinji, Junpei; Bae, Sun-Hye; Wilder, Marcy N

    2014-01-01

    Levels of vitellogenin (VG) and vitellogenesis-inhibiting hormone (VIH) in the whiteleg shrimp, Litopenaeus vannamei, were measured by time-resolved fluoroimmunoassay in relation to the molting cycle and ovarian maturation induced by eyestalk ablation. During the molt cycle, VG mRNA expression levels and VG concentrations showed similar patterns of fluctuation. VG levels increased significantly at early intermolt (stage C0) in adults, but not in subadults. Unilateral and bilateral eyestalk ablation increased VG levels in adults, whereas only bilateral eyestalk ablation affected subadults. VIH levels showed contrasting patterns between adults and subadults. In adults, levels were high in late postmolt adults (stage B) and then low thereafter, whereas they increased from postmolt (stage A) to intermolt (stage C0) in subadults and remained high. Unilateral eyestalk ablation increased VIH levels 10 days following ablation in adults, after which levels decreased at 20 days. VIH levels decreased from 10 to 20 days after bilateral ablation. Both unilateral and bilateral ablation led to increased VIH levels in subadults. Eyestalk ablation induced ovarian maturation, but did not reduce VIH concentrations in the hemolymph. This phenomenon was perhaps due to other crustacean hyperglycemic hormone peptides having cross-reactivity with VIH antibodies. This is the first report to quantify concentrations of VG and VIH together in L. vannamei hemolymph, and to examine their relative dynamics.

  10. Total carotenoids and antioxidant activity of fillets and shells (in natura or cooked of “Vila Franca” shrimp (Litopenaeus Schmitti in different intervals of storage under freezing

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    Giselda Macena Lira

    Full Text Available ABSTRACT Shrimps are sources of carotenoids, astaxanthin is the predominant, responsible for their special and desirable properties, as well as for their instability under heat treatment during the domestic preparation, industrial processing or storage under freezing. These can cause discoloration and reduce the beneficial health properties. This study aimed to evaluate the effect of heat treatment and storage under freezing (0, 45 and 90 days on the levels of total carotenoids and stability of the antioxidant activity of ethanolic extracts of fillets and shells, raw and cooked, of the white shrimp (“Vila Franca” Litopenaeus schmitti (Burkenroad, 1938. The antioxidant ability of the extracts was evaluated using the radicals DPPH• (2,2-diphenyl-1-picryl-hydrazyl and ABTS+• (2,2’-azino-bis (3-ethylbenzothiazoline-6 sulfonic acid, as well as by the iron reducing power (FRAP test. The extracts of cooked or in natura shrimps (fillets and shells represent dietary sources of carotenoids, displaying antioxidant activity through all the tested methods, after heat treatment and storage under freezing. The antioxidant activity of the extracts was superior to the one of ascorbic acid, mainly in the cooked fillet and shells. The samples of shrimp shells seemed a valuable source of carotenoids, whose antioxidant activity was verified even 90 days after freezing, and can be used in food products as functional natural supplement, adding value to this waste.

  11. Evaluation of the presence of Vibrio parahaemolyticus in white shrimp (Litopenaeus vannamei estuarine-wild from southern Sinaloa and northern Nayarit by microbiological analysis and PCR

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    Méndez-Gómez, E.

    2014-01-01

    Full Text Available Given the incidence of human poisonings attributed to raw shrimp consumption in southern of Sinaloa and northern of Nayarit in recent years, white shrimp (Litopenaeus vannamei was sampled from three wildestuaries where it’s been captured and one sample was taken from a sale center in order to determine the possible presence of toxigenic Vibrio parahaemolyticus, from May to December of 2012. Samples were analyzed by Biochemical test and Polymerase Chain Reaction (PCR, they were also tested for the specific an toxicological identification, using molecular oligo nucleotides markers tlh, tdh and trh. The number of positives results were recorded to a table of most probable number (MPN.The number of samples positive for V. parahaemolyticus, weren’t toxigenic and it was demonstrated that they were below the limit established in the Mexican Official Standard NOM-242-SSA1-2009. In conclusion, the present study revealed that consumption of raw shrimp from the dates and sites sampled did not show to be a risk for human gastrointestinal diseases.

  12. Cloning of sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA) gene from white shrimp, Litopenaeus vannamei and its expression level analysis under salinity stress.

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    Wang, Yanhong; Luo, Peng; Zhang, Lvping; Hu, Chaoqu; Ren, Chunhua; Xia, Jianjun

    2013-11-01

    Sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA) is an intracellular membrane bound enzyme that utilizes the free energy of ATP to transport Ca(2+) against a concentration gradient. In the present study, a new SERCA gene (LvSERCA) from white shrimp (Litopenaeus vannamei) was cloned using suppression subtractive hybridization and rapid amplification of cDNA ends. The full-length cDNA of LvSERCA contained an open reading frame of 3,009 bp coding for 1,002 amino acids with a calculated molecular weight of approximately 109.8 kDa. The identity analysis of the amino acid sequence of LvSERCA showed that it is highly conserved with 10 transmembrane α-helices, one P-domain, one A-domain and one N-domain. The phylogenetic analysis revealed that LvSERCA is similar to other Arthropoda SERCA proteins. The mRNA levels of LvSERCA under salinity stress (3 and 40 g L(-1)) were analyzed by reverse transcription PCR and quantitative real-time PCR. The results showed that LvSERCA was expressed in all tissues detected. LvSERCA mRNA levels were significantly higher under hyper-salinity than hypo-salinity. These results highlight that Ga(2+)-ATPase plays an essential role in adjustment salinity stress, which may be useful for selective breeding of L. vannamei.

  13. Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage

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    Xie, Shijun; Li, Fuhua; Zhang, Xiaojun; Zhang, Jiquan; Xiang, Jianhai

    2017-05-01

    The peritrophic membrane plays an important role in the defense system of the arthropod gut. The digestive tract is considered one of the major tissues targeted by white spot syndrome virus (WSSV) in shrimp. In this study, the nucleotide sequence encoding peritrophin-like protein of Litopenaeus vannamei (LvPT) was amplified from a yeast two-hybrid library of L. vannamei. The epitope peptide of LvPT was predicted with the GenScript OptimumAntigen™ design tool. An anti-LvPT polyclonal antibody was produced and shown to specifically bind a band at 27 kDa, identified as LvPT. The LvPT protein was expressed and its concentration determined. LvPT dsRNA (4 μg per shrimp) was used to inhibit LvPT expression in shrimp, and a WSSV challenge experiment was then performed with reverse gavage. The pleopods, stomachs, and guts were collected from the shrimp at 0, 24, 48, and 72 h post-infection (hpi). Viral load quantification showed that the levels of WSSV were significantly lower in the pleopods, stomachs, and guts of shrimp after LvPT dsRNA interference than in those of the controls at 48 and 72 hpi. Our results imply that LvPT plays an important role during WSSV infection of the digestive tract.

  14. RNA-Seq reveals the dynamic and diverse features of digestive enzymes during early development of Pacific white shrimp Litopenaeus vannamei.

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    Wei, Jiankai; Zhang, Xiaojun; Yu, Yang; Li, Fuhua; Xiang, Jianhai

    2014-09-01

    The Pacific white shrimp (Litopenaeus vannamei), with high commercial value, has a typical metamorphosis pattern by going through embryo, nauplius, zoea, mysis and postlarvae during early development. Its diets change continually in this period, and a high mortality of larvae also occurs in this period. Since there is a close relationship between diets and digestive enzymes, a comprehensive investigation about the types and expression patterns of all digestive enzyme genes during early development of L. vannamei is of considerable significance for shrimp diets and larvae culture. Using RNA-Seq data, the types and expression characteristics of the digestive enzyme genes were analyzed during five different development stages (embryo, nauplius, zoea, mysis and postlarvae) in L. vannamei. Among the obtained 66,815 unigenes, 296 were annotated as 16 different digestive enzymes including five types of carbohydrase, seven types of peptidase and four types of lipase. Such a diverse suite of enzymes illustrated the capacity of L. vannamei to exploit varied diets to fit their nutritional requirements. The analysis of their dynamic expression patterns during development also indicated the importance of transcriptional regulation to adapt to the diet transition. Our study revealed the diverse and dynamic features of digestive enzymes during early development of L. vannamei. These results would provide support to better understand the physiological changes during diet transition. Copyright © 2014 Elsevier Inc. All rights reserved.

  15. Efektivitas Pemberian Bacillus sp. D2.2 pada Media Teknis Molase terhadap Kualitas Air dan Performa Pertumbuhan Udang Vaname (Litopenaeus vannamei

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    Ayu NOVITASARI

    2017-07-01

    Full Text Available Vanamei prawns have fast growth and can reared in high density. it has an impact on water quality deterioration and disruption of survival rates and growth. Various ways to cope has been done, one of them is with probiotic bacteria. The new strain of D2.2 bacteria is thought to be effective of ammonia utilization. Probiotics with local bacteria Bacillus sp. D2.2 is cultured on molasses technical medium to be applied semi-mass. The purpose of this study is to asses the efectivity of Bacillus sp. D2.2 inthe molasses technical medium on water quality and growth performance of vaname prawns (Litopenaeus vannamei. The research was used complete randomized design (RAL with four treatments, A (Control, B (Application of 5 ppm Bacillus sp. D2.2 cultured in molasses technical medium, C (Application of 10 ppm Bacillus sp. D2.2 cultured in molasses technical medium, D (Application of 15 ppm Bacillus sp. D2.2 cultured in molasses technical medium were repeated three times each. The results showed no effect on water quality and shrimp survival rate, but absolute growth (W, daily growth rate (GR and feed conversion ratio (FCR showed that B and C treatment had better  than control. Keywords: Vaname shrimp, growth, Bacillus sp. D2.2, molasses technical medium

  16. Effect of Potential Probiotic Lactococcus lactis Subsp. lactis on Growth Performance, Intestinal Microbiota, Digestive Enzyme Activities, and Disease Resistance of Litopenaeus vannamei.

    Science.gov (United States)

    Adel, Milad; El-Sayed, Abdel-Fattah M; Yeganeh, Sakineh; Dadar, Maryam; Giri, Sib Sankar

    2017-06-01

    The aims of this study were to evaluate the effects of Lactococcus lactis subsp. lactis on the growth, intestinal microbiota, digestive enzyme activity, and disease resistance of Litopenaeus vannamei. Diets containing four different concentrations of L. lactis (0 [basal diet], 106, 107, and 108 CFU g-1) were fed to white shrimps L. vannamei (average weight 5.89 ± 0.36 g) for 8 weeks. At the end of the feeding trial, shrimps were immersed in Caspian Seawater (10.8 ppt) contaminated with 106 CFU ml-1 pathogenic V. anguillarum for 2 h. Results revealed that growth rate, survival, and body protein level were increased with dietary supplementation of L. lactis. The activities of digestive enzymes (cellulose, lipase, amylase, and protease) were significantly higher in the groups fed with diets containing 107 or 108 CFU g-1 L. lactis than those in the control. The Lactobacillus and Bacillus counts were higher (P vannamei fed diet supplemented with 108 CFU g-1 of L. lactis exhibited significantly the highest hematocyte count and post-challenge survival rate (79.2 %). Collectively, these results suggest that dietary supplementation of L. lactis subsp. lactis at 108 CFU g-1 can promote growth performance, digestive enzyme activity, and disease resistance of L. vannamei.

  17. Selectively enhanced expression of prophenoloxidase activating enzyme 1 (PPAE1 at a bacteria clearance site in the white shrimp, Litopenaeus vannamei

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    Jang In-Kwon

    2011-12-01

    Full Text Available Abstract Background The prophenoloxidase-activating (PO activating system plays an important role in the crustacean innate immunity, particularly in wound healing and pathogen defense. A key member of this system is prophenoloxidase-activating enzyme (PPAE, which is the direct activator of prophenoloxidase (proPO. Despite their importance in crustacean PO activating system, the studies on them remain limited. Results Here we report on a PPAE of white shrimp, Litopenaeus vannamei (lvPPAE1, which showed 94% similarity to PPAE1 of Penaeus monodon. We found that lvPPAE1 in fluid hemocytes was down regulated after challenge by Vibrio harveyi but was enhanced when shrimps were exposed to a bacteria-rich environment for long-term. In vivo gene silence of lvPPAE1 by RNAi can significantly reduce the phenoloxidase activity (PO and increase the susceptibility of shrimps to V. harveyi. Although lvPPAE1 was down-regulated in fluid hemocytes by Vibrio challenge, its expression increased significantly in gill after bacteria injection, which is the primary bacteria-clearance tissue. Conclusion Suppressed expression in fluid hemocytes and enhanced expression in gill indicates selectively enhanced expression at the bacterial clearance site. This is a novel feature for PPAE expression. The results will contribute to our understanding of the PO activating system in crustaceans.

  18. Involvement of second messengers in the signaling pathway of vitellogenesis-inhibiting hormone and their effects on vitellogenin mRNA expression in the whiteleg shrimp, Litopenaeus vannamei.

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    Bae, Sun-Hye; Okutsu, Tomoyuki; Tsutsui, Naoaki; Kang, Bong Jung; Chen, Hsiang-Yin; Wilder, Marcy N

    2017-05-15

    We incubated fragments of Litopenaeus vannamei ovary to investigate second messengers involved in the regulation of vitellogenin (vg) mRNA levels. The use of 100nM recombinant vitellogenesis-inhibiting hormone (VIH) (corresponding to recombinant L. vannamei sinus gland peptide-G: rLiv-SGP-G) significantly reduced vg mRNA expression in sub-adults after 8h incubation to less than 20% of the control. The concentration of intracellular cyclic guanosine monophosphate (cGMP) increased 3.2-fold relative to the control after 2h incubation with rLiv-SGP-G. However, it reached levels 18-fold relative to the control after 0.5h incubation with rLiv-SGP-G where 3-isobutyl-1-methylxanthine (a phosphodiesterase inhibitor) was also added. Moreover, vg mRNA expression was significantly reduced to less than 50% of the control after 24h incubation with 1μM A23187 (a calcium ionophore). Thus, rLiv-SGP-G and calcium ionophore reduced vg mRNA expression in in vitro-cultured ovary, and cGMP may be involved in the signaling pathway of VIH. Overall, the above results suggest that vg mRNA expression might be inhibited in vitro by increasing intracellular cGMP and Ca2+ in L. vannamei ovary. Copyright © 2017 Elsevier Inc. All rights reserved.

  19. Innate immune-stimulating and immune genes up-regulating activities of three types of alginate from Sargassum siliquosum in Pacific white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Yudiati, Ervia; Isnansetyo, Alim; Murwantoko; Ayuningtyas; Triyanto; Handayani, Christina Retna

    2016-07-01

    The Total Haemocyte Count (THC), phenoloxidase (PO), Superoxide Dismutase (SOD) activity, Phagocytic Activity/Index and Total Protein Plasma (TPP) were examined after feeding the white shrimp Litopenaeus vannamei with diets supplemented with three different types of alginates (acid, calcium and sodium alginates). Immune-related genes expression was evaluated by quantitative Real Time PCR (qRT-PCR). Results indicated that the immune parameters directly increased according to the doses of alginates and time. The 2.0 g kg(-1) of acid and sodium alginate treatments were gave better results. Four immune-related genes expression i.e. LGBP, Toll, Lectin, proPO were up regulated. It is therefore concluded that the supplementation of alginate of Sargassum siliquosum on the diet of L. vannamei enhanced the innate immunity as well as the expression of immune-related genes. It is the first report on the simultaneous evaluation of three alginate types to enhance innate immune parameters and immune-related genes expression in L. vannamei. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Effects of organic acids and essential oils blend on growth, gut microbiota, immune response and disease resistance of Pacific white shrimp (Litopenaeus vannamei) against Vibrio parahaemolyticus.

    Science.gov (United States)

    He, Wangquan; Rahimnejad, Samad; Wang, Ling; Song, Kai; Lu, Kangle; Zhang, Chunxiao

    2017-11-01

    An 8-week feeding trial was undertaken to evaluate supplemental effects of AviPlus® (AP), a blend of organic acids [citric acid, 25%; sorbic acid, 16.7%] and essential oils [thymol, 1.7%; vanillin, 1.0%], on growth, gut microbiota, innate immunity and disease resistance of Pacific white shrimp (Litopenaeus vannamei) against Vibrio parahaemolyticus. A basal experimental diet was formulated and supplemented with 0, 0.3, 0.6, 0.9 and 1.2 g kg-1 AP to produce five test diets (Con, AP0.3, AP0.6, AP0.9 and AP1.2). Each diet was fed to triplicate groups of shrimp (0.2 ± 0.01 g, mean ± SE) to apparent satiation three times daily. Growth performance and survival rate were not significantly influenced by AP supplementation (P > 0.05). Significantly (P vannamei. Copyright © 2017 Elsevier Ltd. All rights reserved.

  1. First Litopenaeus vannamei WSSV 100% oral vaccination protection using CotC::Vp26 fusion protein displayed on Bacillus subtilis spores surface.

    Science.gov (United States)

    Valdez, A; Yepiz-Plascencia, G; Ricca, E; Olmos, J

    2014-08-01

    Litopenaeus vannamei do not have an adaptive immune response system. White spot syndrome virus (WSSV) is the most severe pathogen in shrimps. Bacillus subtilis spores carrying heterologous antigens on its surface have been evaluated as a vaccine inducing specific systemic responses on vertebrates. Orally administrated Vp28 vaccines have been investigated in crustaceans. Vp26 is also an important constituent of WSSV structure but little is known about its oral vaccination capacity in L. vannamei. In this study, for first time, L. vannamei WSSV protection was carried out using B. subtilis recombinant spores (RS), displaying CotC::Vp26 fusion protein (FP) on its surface. RS-expressing FP were coated on shrimp food pellets and used to feed L. vannamei. Results have shown that orally administered B. subtilis RS protected 100% L. vannamei against WSSV infection. Bacillus subtilis spores orally administrated expressing CotC::Vp26 fusion protein on its surface demonstrated the great capacity of Vp26 to induce immune protection, equally or even greater than Vp28 in L. vannamei. The biotechnological process developed represents an easy to produce, practical to handle, environmentally stable, human-safe and economically feasible opportunity to apply a new Vp26 vaccine in a massively way in shrimp farms. © 2014 The Society for Applied Microbiology.

  2. Genome survey and high-density genetic map construction provide genomic and genetic resources for the Pacific White Shrimp Litopenaeus vannamei

    Science.gov (United States)

    Yu, Yang; Zhang, Xiaojun; Yuan, Jianbo; Li, Fuhua; Chen, Xiaohan; Zhao, Yongzhen; Huang, Long; Zheng, Hongkun; Xiang, Jianhai

    2015-01-01

    The Pacific white shrimp Litopenaeus vannamei is the dominant crustacean species in global seafood mariculture. Understanding the genome and genetic architecture is useful for deciphering complex traits and accelerating the breeding program in shrimp. In this study, a genome survey was conducted and a high-density linkage map was constructed using a next-generation sequencing approach. The genome survey was used to identify preliminary genome characteristics and to generate a rough reference for linkage map construction. De novo SNP discovery resulted in 25,140 polymorphic markers. A total of 6,359 high-quality markers were selected for linkage map construction based on marker coverage among individuals and read depths. For the linkage map, a total of 6,146 markers spanning 4,271.43 cM were mapped to 44 sex-averaged linkage groups, with an average marker distance of 0.7 cM. An integration analysis linked 5,885 genome scaffolds and 1,504 BAC clones to the linkage map. Based on the high-density linkage map, several QTLs for body weight and body length were detected. This high-density genetic linkage map reveals basic genomic architecture and will be useful for comparative genomics research, genome assembly and genetic improvement of L. vannamei and other penaeid shrimp species. PMID:26503227

  3. Effect of pure microcystin-LR on activity and transcript level of immune-related enzymes in the white shrimp (Litopenaeus vannamei).

    Science.gov (United States)

    Chen, Yanyan; Huang, Xianghu; Wang, Jianzhu; Li, Changling

    2017-07-01

    Microcystins (MCs) in freshwater and marine waters released by toxin-producing cyanobacteria have negative impacts to the aquatic environment. This study aimed to investigate the effect of pure microcystin-LR on activity and transcript level of immune-related enzymes in the white shrimp Litopenaeus vannamei. After exposed to varying concentrations of pure microcystin-LR (MC-LR) for 30 days, the activity of superoxide dismutase (SOD), lysozyme (LZM), glutathione peroxidase (GPx), peroxidase (POD), acid phosphatase (ACP), alkaline phosphatase (AKP) and transcript level of cMn-sod, lzm, gpx were investigated in the hepatopancreas of white shrimp (L. vannamei). Immune-related enzyme activities responded differently to MC-LR exposure. SOD, GPx, and POD activity in the hepatopancreas were activated in a concentration-dependent manner while LZM activity was significantly inhibited in the treatment groups. ACP and AKP activity showed an increase, followed by a decrease. The transcript levels of cMn-sod, lzm, and gpx were consistent with changes in their encoding enzyme activity. These results demonstrated that sub-chronical exposure to MC-LR induced the alteration of immune-related enzymes and corresponding genes in the hepatopancreas, which may help explain the presence of detoxification mechanisms in crustaceans and how they were protected from MC-LR stress for a long period of time.

  4. Characterization of Four Novel Caspases from Litopenaeus vannamei (Lvcaspase2-5) and Their Role in WSSV Infection through dsRNA-Mediated Gene Silencing

    Science.gov (United States)

    Wang, Pei-Hui; Wan, Ding-Hui; Chen, Yong-Gui; Weng, Shao-Ping; Yu, Xiao-Qiang; He, Jian-Guo

    2013-01-01

    Apoptosis plays an important role in white spot syndrome virus (WSSV) pathogenesis, and caspases are central players in apoptosis. Here, we cloned four novel caspases (Lvcaspase2-5) from the Pacific white shrimp Litopenaeus vannamei, and investigated their potential roles in WSSV replication using dsRNA-mediated gene silencing. Lvcaspase2-5 have the typical domain structure of caspase family proteins, with the conserved consensus motifs p20 and p10. Lvcaspase2 and Lvcaspase5 were highly expressed in muscle, while Lvcaspase3 was highly expressed in hemocytes and Lvcaspase4 was mainly expressed in intestine. Lvcaspase2-5 could also be upregulated by WSSV infection, and they showed different patterns in various tissues. When overexpressed in Drosophila S2 cells, Lvcaspase2-5 showed different cellular localizations. Using dsRNA-medicated gene silencing, the expression of Lvcaspase2, Lvcaspase3, and Lvcaspase5 were effectively knocked down. In Lvcaspase2-, Lvcaspase3- or Lvcaspase5-silenced L. vannamei, expression of WSSV VP28 gene was significantly enhanced, suggesting protective roles for Lvcaspase2, Lvcaspase3 and Lvcaspase5 in the host defense against WSSV infection. PMID:24376496

  5. Transcriptomic and morphological analyses of Litopenaeus vannamei intestinal barrier in response to Vibrio paraheamolyticus infection reveals immune response signatures and structural disruption.

    Science.gov (United States)

    Qi, Cancan; Wang, Lei; Liu, Mei; Jiang, Keyong; Wang, Mengqiang; Zhao, Wei; Wang, Baojie

    2017-11-01

    The white shrimp Litopenaeus vannamei has been greatly impacted by Vibrio infection. In this study, we investigated the intestinal barrier response of L vannamei following challenge with Vibrio parahaemolyticus E1, by examining morphological changes and transcriptome expression levels. A total of 16,4420 unigenes were obtained from RNAseq data after quality control and assembly, and 4646 differentially expressed genes (DEGs) were identified following Vibrio challenge, of which 2469 unigenes were significantly up-regulated and 2177 were significantly down-regulated. DEGs were determined to be involved in various physical, chemical and immunological intestinal barrier functions, including peritrophin, cytoskeleton and cell junction, pattern recognition receptors, antimicrobial peptide and immune signaling pathways, serine protease/protease inhibitor and prophenoloxidase system, apoptosis and phagocytosis, and antioxidant systems. Fifteen DEGs were randomly selected for validation by real-time quantitative PCR (RT-qPCR) and showed results consistent with the RNA-seq data. Intestinal epithelial cell morphology was also affected by Vibrio challenge, showing epithelial detachment, nuclear pyknosis, and destruction of cell junctions. These results improve our current understanding of the intestinal barrier function in the shrimp response to bacterial infection. Copyright © 2017 Elsevier Ltd. All rights reserved.

  6. IDENTIFICATION OF A LOCAL PROBIOTIC BACTERIUM USING 16S rRNA GENE SEQUENCE THAT WAS USED FOR FIELD TRIAL TO ENHANCED WHITELEG SHRIMP (Litopenaeus vannamei SURVIVAL

    Directory of Open Access Journals (Sweden)

    Tb. Haeru Rahayu

    2015-12-01

    Full Text Available The use of local probiotics in the culture of aquatic organisms is increasing with the demand for more environmental-friendly aquaculture practices. The local bacterium isolate considered as a probiotic was added into the water of whiteleg shrimp (Litopenaeus vannamei culture in a field trial. Four rectangular plastic ponds (ca. 20 m x 30 m per pond were used for 100 days experimentation for six consecutive crops in two years experiment. Survival, harvest size, feed conversion ratio (FCR and Vibrio bacterial count was compared with those of shrimp receiving and none of local isolate. Identification based on 16S rRNA gene sequence shown those isolate was Bacillus pumilus strain DURCK14 with 99% homology. Water shrimp pond added a local isolate had significantly higher survival at about 10.0% to 11.7% than shrimp without added the isolate (p<0.05, and better FCR, but no significant different in shrimp harvest size. Vibrio bacterial was undetected by total plate count. Moreover, it shown better projected yields on an annual basis (three crops per year.

  7. Isolation and cDNA cloning of a novel red colour-related pigment-binding protein derived from the shell of the shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Pan, Chuang; Ishizaki, Shoichiro; Nagashima, Yuji; Gao, Jialong; Watabe, Shugo

    2018-02-15

    Pigment-binding proteins play important roles in crustacean shell colour change. In this study, a red colour-related pigment-binding protein, designated LvPBP75, was purified from the shell of Litopenaeus vannamei. HPLC and PAGE analysis showed that LvPBP75 was a homogeneous monomer with molecular mass of 75kDa. Peptide mass fingerprint analysis revealed that LvPBP75 belonged to hemocyanin, and the released pigment from heated LvPBP75 showed a λmax at 481nm in acetone. The significant red-colour change temperatures were detected at 30 and 80°C, respectively. Based on the determined amino acid fragments, a full-length cDNA of LvPBP75 was cloned and sequenced. The ORF encodes a protein of 662 amino acids having 80% identity with penaeidae hemocyanin. These results strongly suggest a novel function of hemocyanin, namely binding with pigment, and its involvement in L. vannamei shell colour change. Copyright © 2017 Elsevier Ltd. All rights reserved.

  8. Litopenaeus vannamei Sterile-Alpha and Armadillo Motif Containing Protein (LvSARM) Is Involved in Regulation of Penaeidins and antilipopolysaccharide factors

    Science.gov (United States)

    Wang, Pei-Hui; Gu, Zhi-Hua; Wan, Ding-Hui; Zhu, Wei-Bin; Qiu, Wei; Weng, Shao-Ping; Yu, Xiao-Qiang; He, Jian-Guo

    2013-01-01

    The Toll-like receptor (TLR)-mediated NF-κB pathway is tightly controlled because overactivation may result in severe damage to the host, such as in the case of chronic inflammatory diseases and cancer. In mammals, sterile-alpha and armadillo motif-containing protein (SARM) plays an important role in negatively regulating this pathway. While Caenorhabditis elegans SARM is crucial for an efficient immune response against bacterial and fungal infections, it is still unknown whether Drosophila SARM participates in immune responses. Here, Litopenaeus vannamei SARM (LvSARM) was cloned and functionally characterized. LvSARM shared signature domains with and exhibited significant similarities to mammalian SARM. Real-time quantitative PCR analysis indicated that the expression of LvSARM was responsive to Vibrio alginolyticus and white spot syndrome virus (WSSV) infections in the hemocyte, gill, hepatopancreas and intestine. In Drosophila S2 cells, LvSARM was widely distributed in the cytoplasm and could significantly inhibit the promoters of the NF-κB pathway-controlled antimicrobial peptide genes (AMPs). Silencing of LvSARM using dsRNA-mediated RNA interference increased the expression levels of Penaeidins and antilipopolysaccharide factors, which are L.vannamei AMPs, and increased the mortality rate after V. alginolyticus infection. Taken together, our results reveal that LvSARM may be a novel component of the shrimp Toll pathway that negatively regulates shrimp AMPs, particularly Penaeidins and antilipopolysaccharide factors. PMID:23405063

  9. Growth and feed efficiency of juvenile shrimp Litopenaeus vannamei fed formulated diets containing different levels of poultry by-product meal

    Science.gov (United States)

    Chi, Shuyan; Tan, Beiping; Mai, Kangsen; Zheng, Shixuan

    2009-12-01

    This feeding trial was conducted to evaluate the potential of poultry by-product meal (PBM) as a protein source in the culture of Litopenaeus vannamei. Seven isonitrogenous and isoenergetic diets were formulated to near to commercial diet with about 40% protein and 7.5% lipid. Fish meal was replaced by 0, 30%, 40%, 50%, 60%, 70% and 100% of PBM (diets 1-7). The diet with 100% fish meal was used as a control (diet 1). Post-larvae were reared in an indoor semi-closed re-circulating system. Each dietary treatment was tested in 4 replicate tanks (260 L) of 40 shrimp, arranged in a completely randomized design. The shrimps were hand-fed for three times a day to near-satiation (0700, 1200 and 1800) for 60 d. Percentage weight gain, survival, feed conversion ratio (FCR), protein efficiency ratio (PER) and body composition of shrimps were measured. There were no significant differences ( P>0.05) in growth performance among shrimps fed diets 1-5 (0-60% fish meal replacement). However, shrimps fed diet 7 (100% fish meal replacement) had significantly lower ( P0.05) among different experimental diets. No differences in body composition were found among shrimps fed different diets. These results showed that up to 70% of fish meal protein can be replaced by PBM without adversely affecting the growth, survival, FCR, PER and body composition of Litop enaeus vannamei.

  10. Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage

    Science.gov (United States)

    Xie, Shijun; Li, Fuhua; Zhang, Xiaojun; Zhang, Jiquan; Xiang, Jianhai

    2017-11-01

    The peritrophic membrane plays an important role in the defense system of the arthropod gut. The digestive tract is considered one of the major tissues targeted by white spot syndrome virus (WSSV) in shrimp. In this study, the nucleotide sequence encoding peritrophin-like protein of Litopenaeus vannamei (LvPT) was amplified from a yeast two-hybrid library of L. vannamei. The epitope peptide of LvPT was predicted with the GenScript OptimumAntigen™ design tool. An anti-LvPT polyclonal antibody was produced and shown to specifically bind a band at 27 kDa, identified as LvPT. The LvPT protein was expressed and its concentration determined. LvPT dsRNA (4 μg per shrimp) was used to inhibit LvPT expression in shrimp, and a WSSV challenge experiment was then performed with reverse gavage. The pleopods, stomachs, and guts were collected from the shrimp at 0, 24, 48, and 72 h post-infection (hpi). Viral load quantification showed that the levels of WSSV were significantly lower in the pleopods, stomachs, and guts of shrimp after LvPT dsRNA interference than in those of the controls at 48 and 72 hpi. Our results imply that LvPT plays an important role during WSSV infection of the digestive tract.

  11. Combined use of DGT and transplanted shrimp (Litopenaeus vannamei) to assess the bioavailable metals of complex contamination: implications for implementing bioavailability-based water quality criteria.

    Science.gov (United States)

    Wang, Zaosheng; Zhao, Peihong; Yan, Changzhou; Chris, Vulpe D; Yan, Yijun; Chi, Qiaoqiao

    2014-03-01

    The diffusive gradients in thin films (DGT) were field deployed alongside the shrimp Litopenaeus vannamei at seven sites with different levels of contamination to assess the potentially bioavailable and toxic fraction of metal contaminants. After 7 days of exposure, several antioxidant biomarkers were quantified in hepatopancreas of exposed shrimps, and tissue levels as well as the total, dissolved, and DGT-labile concentrations of metal contaminants were determined in the pooled site samples. The results showed that the caged shrimps had high tissue contaminant concentrations and significantly inhibited antioxidant responses at the more contaminated sites. DGT-labile metal concentrations provided better spatial resolution of differences in metal contamination when compared with traditional bottle sampling and transplanted shrimp. The total, dissolved, and DGT-labile metal fractions were used to evaluate the potential bioavailability of metal contaminants, comparing with metal accumulation and further linking to antioxidant biomarker responses in tissues of exposed shrimps. Regression analysis showed the significant correlations between DGT-Cu concentrations and tissue-Cu and activities of some biomarker responses in the shrimp hepatopancreas. This indicated that DGT-labile Cu concentrations provided the better prediction of produced biological effects and of the bioavailability than the total or dissolved concentrations. The study supports the use of methods combining transplanted organisms and passive sampling for assessing the chemical and ecotoxicological status of aqueous environments and demonstrates the capability of the DGT technique as a powerful tool for measuring the bioavailability-based water quality in variable coastal environments.

  12. Functional Feed Assessment on Litopenaeus vannamei Using 100% Fish Meal Replacement by Soybean Meal, High Levels of Complex Carbohydrates and Bacillus Probiotic Strains

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    Rosalia Contreras

    2011-06-01

    Full Text Available Functional feed supplemented with alternative-economic nutrient sources (protein, carbohydrates, lipids and probiotics are being considered in shrimp/fish aquaculture production systems as an option to increase yield and profits and to reduce water pollution. In this study the probiotic potential to formulate functional feeds have been evaluated using four dietary treatments: Treatment 1 (B + Bs; Bacillus subtilis potential probiotic strain was supplemented to a soybeanmeal (SBM—carbohydrates (CHO basal feed. Treatment 2 (B + Bm; Bacillus megaterium potential probiotic strain was supplemented to the same SBM-CHO basal feed. In Treatment 3 (B; SBM-CHO basal feed was not supplemented with probiotic strains. Treatment 4 (C; fishmeal commercial feed (FM was utilized as positive control. Feeding trials evaluated the survival, growth, and food conversion ratio and stress tolerance of juvenile Litopenaeus vannamei (Boone Pacific white shrimp. Best overall shrimp performance was observed for animals fed with Treatment 1 (B+Bs; additionally, stress tolerance and hemolymph metabolites also showed the best performance in this treatment. SBM-CHO basal feed not supplemented with probiotic strains (B presented smaller growth and lower feed conversion ratio (FCR. Shrimps fed with the fishmeal commercial feed (C presented the lowest stress tolerance to high ammonia and low oxygen levels. Specifically selected B. subtilis strains are recommended to formulate functional and economical feeds containing high levels of vegetable; protein and carbohydrates as main dietary sources in L. vannamei cultures.

  13. EFEITO DAS ALTAS DENSIDADES DE ESTOCAGEM NO CRESCIMENTO E SOBREVIVÊNCIA DE Litopenaeus vannamei NA FASE FINAL DE ENGORDA, CULTIVADOS EM SISTEMAS DE BIOFLOCOS (BFT

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    Adriana Ferreira Silva

    2013-09-01

    Full Text Available The aim of this study was to evaluate the effect of high stocking densities on survival, growth and feed conversion rates of Litopenaeus vannamei shrimp, in final growout phase, in a Biofloc Technology (BFT culture system, keeping the same water parameters for all treatments. Shrimps (11.96 ± 1.14 g were stocked in microcosms (0.50/m2 tanks, connected to a BFT system raceway. The study was carried out for 45 days. The shrimp were stocked at densities of 150, 300, 450 and 600 shrimp/m2. Bioflocs were collected for analysis of proximate composition. The results were submitted to one-way ANOVA, and differences were compared by Tukey test (α = 0.05. In T300 and T450, growth and survival were not affected by high stocking densities. The highest biomass reached (T450 was 5.1kg/m² and the best feed conversion rate was 1.54 in T150. The results of this study indicate that stocking densities in the proposed system can be high, but not exceeding 450 shrimp/m². Furthermore, even maintaining the same water parameters for all treatments, there was a negative effect between density and shrimp growth, confirming that this effect is behavioral.

  14. SUBSTITUIÇÃO DE RAÇÃO NO CRESCIMENTO DE JUVENIS DO CAMARÃO MARINHO Litopenaeus vannamei EM LABORATÓRIO

    Directory of Open Access Journals (Sweden)

    CIBELE SOARES PONTES

    2010-01-01

    Full Text Available To evaluate the effect of the temporary replacement of shrimp ration (RC by chicken ration (RF in the culture of Litopenaeus vannamei shrimp, juvenils (1.07 ± 0.26 g, were cultivated (52/m2 for 30 days in 50 L boxes, with constant aeration, 0% of water exchange and fed with 10% of its biomass/day at 08:00 and 16:00 h, according to the treatments: (1 RC; (2 1 week RC + 1 week RF, alternate; (3 RF and (4 1 day RF + 1 day RC, alternate; with 5 repetitions each. There was no difference (P <0.05 in the weight of shrimp on the basis of treatments applied at the beginning (Kruskal-Wallis, P = 0003, 15 days (Kruskal-Wallis, P = 0.98 and at 30 days (Kruskal-Wallis, P = 0.07 to experiment. The Specific Growth Rate (rm ANOVA, P = 0.28 and survival (Friedman, P = 0,34 the animals were significantly similar, indicating that these parameters were not affected by treatments, with the possible replacement of shrimp ration by chicken ration, as a measure of containment of expenditure, for short intervals of time. Studies are needed to validate these results in earth ponds.

  15. Crystallographic Studies Evidencing the High Energy Tolerance to Disrupting the Interface Disulfide Bond of Thioredoxin 1 from White Leg Shrimp Litopenaeus vannamei

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    Adam A. Campos-Acevedo

    2014-12-01

    Full Text Available Thioredoxin (Trx is a small 12-kDa redox protein that catalyzes the reduction of disulfide bonds in proteins from different biological systems. A recent study of the crystal structure of white leg shrimp thioredoxin 1 from Litopenaeus vannamei (LvTrx revealed a dimeric form of the protein mediated by a covalent link through a disulfide bond between Cys73 from each monomer. In the present study, X-ray-induced damage in the catalytic and the interface disulfide bond of LvTrx was studied at atomic resolution at different transmission energies of 8% and 27%, 12.8 keV at 100 K in the beamline I-24 at Diamond Light Source. We found that at an absorbed dose of 32 MGy, the X-ray induces the cleavage of the disulfide bond of each catalytic site; however, the interface disulfide bond was cleaved at an X-ray adsorbed dose of 85 MGy; despite being the most solvent-exposed disulfide bond in LvTrx (~50 Å2. This result clearly established that the interface disulfide bond is very stable and, therefore, less susceptible to being reduced by X-rays. In fact, these studies open the possibility of the existence in solution of a dimeric LvTrx.

  16. Crystallographic studies evidencing the high energy tolerance to disrupting the interface disulfide bond of thioredoxin 1 from white leg shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Campos-Acevedo, Adam A; Rudiño-Piñera, Enrique

    2014-12-15

    Thioredoxin (Trx) is a small 12-kDa redox protein that catalyzes the reduction of disulfide bonds in proteins from different biological systems. A recent study of the crystal structure of white leg shrimp thioredoxin 1 from Litopenaeus vannamei (LvTrx) revealed a dimeric form of the protein mediated by a covalent link through a disulfide bond between Cys73 from each monomer. In the present study, X-ray-induced damage in the catalytic and the interface disulfide bond of LvTrx was studied at atomic resolution at different transmission energies of 8% and 27%, 12.8 keV at 100 K in the beamline I-24 at Diamond Light Source. We found that at an absorbed dose of 32 MGy, the X-ray induces the cleavage of the disulfide bond of each catalytic site; however, the interface disulfide bond was cleaved at an X-ray adsorbed dose of 85 MGy; despite being the most solvent-exposed disulfide bond in LvTrx (~50 Å2). This result clearly established that the interface disulfide bond is very stable and, therefore, less susceptible to being reduced by X-rays. In fact, these studies open the possibility of the existence in solution of a dimeric LvTrx.

  17. Effect of addition of Navicula sp. on plankton composition and postlarvae growth of Litopenaeus vannamei reared in culture tanks with zero water exchange

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    Yllana Ferreira-Marinho

    2014-07-01

    Full Text Available The aim of this study was to evaluate the effect of the addition of Navicula sp. on plankton composition and postlarvae growth of Litopenaeus vannamei reared in culture tanks with zero water exchange systems. Four treatments were considered: zero water exchange (ZWE; ZWE with the addition of feed (ZWE-F; ZWE with the addition of Navicula sp. (ZWE-N and ZWE with the addition of feed and Navicula sp. (ZWE-FN, all in triplicate. Shrimp of 17.7 ± 0.02 mg were stocked at a density of 2500 shrimp m-3 and microalgae added on the 1st, 5th and 15th day at a density of 5x10(4 cell mL-1. The shrimp were fed a commercial feed composed by 42% crude protein four times a day except in the ZWE treatment. For data analysis we used Cochran, Shapiro-Wilk, ANOVA, Tukey and Student-t tests (P < 0.05. The most frequent genera were: Anabaena, Arcella, Asplanchma, Bosmina, Brachionus, Cylindrotheca, Daphnia, Fragilaria, Hemiaulus, Keratella, Orthoseira, Oscillatoria, Phymatodocis, Rhabdonema, Skeletonema, Sckizothrix and Ulothrix. Significant differences between treatments were observed for TAN, NO2-N, alkalinity, final weight, weight gain, final biomass, biomass gain, feed conversion ratio, specific growth rate and survival. The ZWE-FN treatment showed better production parameters, indicating the benefits of the addition of Navicula sp. as a natural food source for L. vannamei postlarvae in zero water exchange systems.

  18. Efectos combinados de las vitaminas c y e dietéticas en la inmunorespuesta del juvenil litopenaeus vannamei antes y después de la suplementación con glucanos

    OpenAIRE

    Molina, César; Rodríguez, Jenny; Echeverría, Fabrizio

    2001-01-01

    Efectos combinados de las vitaminas C y E dietéticas en la inmunorespuesta del juvenil Litopenaeus vannamei antes y después de la suplementación con glucanos Se considera que el estado nutricional tiene una influencia importante en la salud y en la capacidad de los animales para resistir enfermedades. Estudios relacionando la nutrición con la función inmune han sido mayormente desarrollados en peces sin embargo su rol en la resistencia a enfermedades es pobremente entendido.

  19. Efeito da remoção de sólidos suspensos totais e desempenho zootécnico do camarão Litopenaeus vannamei em sistema superintensivo com bioflocos

    OpenAIRE

    Gaona, Carlos Augusto Prata

    2011-01-01

    Dissertação(mestrado) - Universidade Federal do Rio Grande, Programa de Pós-Graduação em Aqüicultura, Instituto de Oceanografia, 2011. A carcinicultura destaca-se pela crescente demanda da atividade, além do alto valor de mercado dos crustáceos. Em função do atrativo comercial, a carcinicultura passou a ser uma alternativa de produção frente a exploração dos recursos naturais. No Brasil, a carcinicultura se intensificou na década de 90 com a espécie Litopenaeus vannamei. Ações tomadas em f...

  20. Evaluation of the nutritional quality of Chaetoceros muelleri Schütt (Chaetocerotales: Chaetocerotaceae and Isochrysis sp. (Isochrysidales: isochrysidaceae grown outdoors for the larval development of Litopenaeus vannamei (Boone, 1931 (Decapoda: Penaeidae

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    Rodríguez Erika O.

    2012-01-01

    Full Text Available The biomass, proximal composition and fatty acid profile of Isochrysis sp., Chaetoceros muelleri and their mixture, grown under greenhouse conditions, were evaluated. The nutritional value of both species supplied as the monoalgal (Chaetoceros muelleri: Diet I, and Isochrysis sp. Diet II and mixed diet (Diet III for larval Litopenaeus vannamei was also assessed on the basis of the development and biochemical composition of the larvae. The highest protein levels were obtained in Diets I and II (40% and 35%, respectively. No significant differences in larval survival were found among the diets; however, larvae fed on Diet II had the lowest mean larval length.

  1. Bacillus NP5 Improves Growth Performance and Resistance Against Infectious Myonecrosis Virus in White Shrimp (Litopenaeus vannamei (Bacillus NP5 Meningkatkan Pertumbuhan dan Ketahanan Terhadap Infeksi Virus Myonecrosis pada Udang Putih (L. vannamei

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    Widanarni Widanarni

    2015-07-01

    Full Text Available Infectious Myonecrosis (IMN merupakan salah satu penyakit yang sering menyerang udang vaname. Probiotik banyak digunakan pada budidaya udang karena terbukti mampu mengurangi serangan penyakit pada udang. Penelitian ini bertujuan untuk menguji pengaruh pemberian probiotik Bacillus NP5 melalui pakan terhadap kinerja pertumbuhan, respons imun, dan resistensi udang vaname terhadap infeksi Infectious Myonecrosis Virus (IMNV. Udang vaname Litopenaeus vannamei (2.41±0.07 g ekor-1 diberi pakan yang disuplementasi probiotik Bacillus NP5 dengan dosis yang berbeda, 102 CFU.g-1 (A, 104 CFU.g-1 (B, 106 CFU.g-1 (C, dan kontrol tanpa suplementasi probiotik (kontrol negatif, KN; kontrol positif, KP selama 30 hari dan dengan tiga ulangan untuk masing-masing dosis, kemudian KP, perlakuan A, B, dan C diuji tantang secara intramuskular dengan IMNV (100 µl.ekor-1. Hasil penelitian ini menunjukkan bahwa udang vaname yang diberi pakan dengan suplementasi probiotik mempunyai laju pertumbuhan harian (LPH, rasio konversi pakan (RKP, dan respons imun yang lebih tinggi. Udang tersebut juga mempunyai total hemocyte count (THC dan resistensi terhadap IMNV yang lebih tinggi dibandingkan kontrol positif. Konsentrasi probiotik 106 CFU.g-1 memberikan hasil terbaik dalam meningkatkan pertumbuhan, respon imun, dan resistensi udang vaname terhadap infeksi IMNV. Kata kunci: probiotik, Bacillus NP5, Litopenaeus vannamei, pertumbuhan, IMNV Infectious Myonecrosis (IMN is one of the most prevalent white shrimp diseases. Probiotics are widely used in shrimp cultivation because they have been proven to reduce shrimp disease outbreak. This study aimed to observe the effect of oraly administered probiotic Bacillus NP5 on the white shrimp's growth performance, immune response, and resistance to Infectious Myonecrosis Virus (IMNV infection. White shrimp Litopenaeus vannamei (2.41±0.07 g individual-1 were fed with a feed supplemented with different doses of the probiotic Bacillus NP5, i

  2. Farinha dos resíduos do camarão Litopenaeus vannamei: caracterização e utilização na formulação de hambúrguer

    OpenAIRE

    Suzanne Florentino da Silva Chaves Damasceno, Karla

    2007-01-01

    O potencial brasileiro para a produção e exportação de camarão Litopenaeus vannamei e o grande volume de resíduo gerado pelo seu beneficiamento motivaram a presente pesquisa com o objetivo de contribuir para o aproveitamento destes e incentivar a utilização da farinha de resíduo de camarão em produtos alimentícios. Foram realizados estudos por meio de planejamento fatorial 23, para definir os parâmetros de fabricação da farinha a partir de resíduos do beneficiamento do camarão ...

  3. Aclimatação do camarão marinho Litopenaeus vannamei (Boone, 1931 à água doce com diferentes estratégias de alimentação e calagem - DOI: 10.4025/actascianimsci.v28i1.668 Acclimatization of the marine shrimp Litopenaeus vannamei (Boone, 1931 to the freshwater with different feeding and liming strategies - DOI: 10.4025/actascianimsci.v28i1.668

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    Bruno Leonardo da Silva Santos

    2006-01-01

    Full Text Available Pós-Larvas do camarão Litopenaeus vannamei, na fase de PL9-10 e PL25-26 foram aclimatadas à água doce (0,0 ‰, objetivando promover cultivos em regiões interioranas. Para isso, foram realizados dois experimentos conduzidos em aquários com capacidade útil de 44 litros e densidade de estocagem de 11,36 pós-larvas/L. No experimento 1, foram testadas diferentes concentrações de cal hidratada (0,018 g a 0,031 g e 2 dietas, formuladas à base de ração comercial para camarão e biomassa de artêmia. No experimento 2, foram testadas 5 dietas com diferentes concentrações de biomassa de artêmia (0% a 100%, inseridas em rações de peixes e camarões. No final do processo de aclimatação, a sobrevivência das póslarvas variou de 40,44% a 98,04%. Concluiu-se que pós-larvas do Litopenaeus vannamei podem ser aclimatadas à água doce e que o aumento da concentração de cal e de artêmia na ração melhoraram significativamente (p Litopenaeus vannamei postlarvae with 9-10 (PL9-10, 25-26 days old (PL25-26 were acclimated to freshwater (0 ppt for the purpose of attending the inland cultures. Two experiments were conducted in 44 liter aquariums with stocking density of 11.36 postlarvae/L. In experiment 01, different concentrations of hydrated lime (0.018 g a 0.031 g and two diets, formulated with commercial shrimp ration and artemia biomass, were tested. In experiment 02, five diets, with different concentrations of artemia biomass (0 to 100% inserted in fish and shrimp rations, were tested. At the end of the acclimatization process, the postlarvae survival varied from 40.44 to 98.04%. Results showed that Litopenaeus vannamei postlarvae can be acclimated to freshwater. Also, the increase of lime concentration and the artemia biomass in the ration improved (p < 0.05 the zootechnic parameters of the species in a significant way.

  4. Descripción del ciclo de vida de una población silvestre de Triatoma dimidiata (Hemiptera: Rediviidae y análisis genético de tres grupos por medio de marcadores bioquímicos

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    Ardila Roldán Susanne Carolina

    2002-11-01

    Full Text Available El insecto de la subfamilia Reduviidae, Triatoma dimidiata, es actualmente en Colombia elsegundo vector más importantes en la transmisión de la enfermedad de Chagas luego de laespecie Rhodnius prolixusquien se halla principalmente en viviendas humanas. Este insecto puedehabitar viviendas rústicas, peridomicilios o sitios aledaños a las viviendas o ambientes silvestres,insectos de los cuales se conoce poco sobre su capacidad vectorial y de infestación. Para es-tudiar algunas aspectos de la biología del insecto, se estudiaron 96 individuos a partir de treshembras del ambiente silvestre para el estudio del ciclo de vida, también se estudió la fecun-didad y fertilidad de las mismas. Se analizó la resistencia al ayuno de 10 insectos de cada unode los estadíos ninfales y los patrones de alimentación y deyección para otros 30 insectos decada estadío. Finalmente se evaluaron 80 individuos de los grupos: domicilio, peridomicilio ysilvestre mediante 10 sistemas isoenzimáticos, con el ánimo de canalizar su filogenia Se obtuvouna fertilidad en las hembras de hasta 97.1%, y una fecundidad máxima de hasta 630 huevospor hembra. El ciclo de vida completo tardó en promedio 356.7 días para las hembras y 368.1para los machos. La resistencia al ayuno para el primer estadío obtuvo un mínimo 18 días y unmáximo 66 días de supervivencia; para el segundo estadío un mínimo 110 días y un máximo de174 días y para el tercer estadío el estudio llegó hasta 174 días como mínimo. El primer estadíoestuvo en capacidad de ingerir mayor cantidad de sangre con respecto a los demás, consu-miendo hasta 12.35 veces su peso inicial. Se observó que el 68% de los individuos defecarondurante e inmediatamente luego de abandonar la fuente de alimento, tardándose en promediotodos los estadíos 42.9 minutos. Genéticamente los tres grupos se comportan como si fuesenuna sola población al no encontrarse distancias altas entre ellos. Se observó una reducci

  5. ¿Es la cobertura forestal conservada y restaurada por las zonas protegidas?: El caso de dos áreas silvestres protegidas en el Pacífico Central de Costa Rica

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    J Antonio Guzmán Q

    2015-09-01

    Full Text Available Resumen:Cambios en el uso del suelo son principalmente consecuencia de las acciones antropogénicas. La actual transformación agrícola y urbana en Costa Rica ha generado preguntas acerca de la efectividad de la conservación y restauración dentro de las áreas protegidas. En este documento nosotros analizamos los patrones de cambio del uso del suelo entre tres periodos: 1997, 2005 y 2010 en términos de magnitud, dirección y velocidad a través de mapas categóricos generados por la foto-interpretación para dos áreas silvestres protegidas y sus áreas aledañas: Parque Nacional La Cangreja (LCNP, el Refugio de Vida Silvestre Rancho Mastatal (RMWR y sus áreas aledañas (SA, esta última compuesta por área de un kilómetro de radio fuera de los límites de las áreas protegidas. La matriz que describe el paisaje dentro de las áreas protegidas es la cobertura natural, compuesta principalmente por la cobertura forestal y tacotales. Encontramos que la cobertura natural más abundante para ambas áreas protegidas fue cubierta forestal en todos los años estudiados. La estabilidad y las grandes áreas de la cubierta forestal en LCNP y RMWR para 2005 y 2010 reflejan que las políticas, las acciones de manejo y vigilancia tienen un impacto positivo en la conservación y restauración de los hábitats naturales en esta zona del Pacifico Central Costarricense. Sin embargo, la alta complejidad del paisaje de SA en 1997, 2005 y 2010 son una prueba de presión antropogénica sobre estas áreas protegidas y sugieren una ineficacia de los gobiernos locales para monitorear y disminuir los cambios de uso del suelo que podrían obstaculizar la gestión, conservación y restauración de especies dentro de las áreas protegidas.

  6. Storage protein profile and amino acid content in wild rice Oryza glumaepatula Perfil da proteína de reserva e conteúdo de aminoácidos no arroz silvestre Oryza glumaepatula

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    Karina Freire D'eça Nogueira Santos

    2013-01-01

    Full Text Available - The objective of this work was to determine the total protein profile and the contents of the four major protein fractions (albumin, globulin, prolamin and glutelin and of the amino acids in the endosperm of the rice wild species Oryza glumaepatula. The experiment was performed with 29 accessions of this species, collected from 13 Brazilian locations, and two commercial cultivars. Protein samples were prepared using dried, polished, and ground grains to obtain homogeneous, dry flour used in the preparation of extracts. Oryza glumaepatula accessions were identified with the highest levels of total protein, albumin and glutelin protein fractions, and amino acids (with the exception of tryptophan in comparison to the two analized rice cultivars. The albumin and glutelin profiles in SDS-Page were distinct between rice cultivars and O. glumaepatula. This wild species has the potential to increase the nutritional quality of rice storage protein through interspecific crosses.- O objetivo deste trabalho foi determinar os perfis de proteína total e o conteúdo das quatro principais frações proteicas (albumina, globulina, prolamina e glutelina e de aminoácidos no endosperma da espécie de arroz silvestre Oryza glumaepatula. O experimento foi realizado com 29 acessos dessa espécie, coletados em 13 locais no Brasil, e duas cultivares comerciais. Amostras de proteínas foram preparadas com grãos secos, polidos e moídos, para obtenção de uma farinha seca e homogênea, usada no preparo dos estratos. Acessos de O. glumaepatula foram identificados com os maiores níveis de proteína total, frações proteicas de albumina e glutelina, e aminoácidos (com exceção do triptofano, em comparação às duas cultivares de arroz avaliadas. Os perfis de albumina e glutelina em SDS-PAGE foram distintos entre as cultivares de arroz e O. glumaepatula. Essa espécie silvestre tem o potencial de aumentar a qualidade nutricional da proteína de reserva do arroz por

  7. Assessing the potential toxicity of marine sediments found in petroleum industry areas: A new approach based on responses of postlarval shrimp; Evaluacion del potencial de toxicidad de sedimentos marinos en areas de la industria petrolera: Un nuevo metodo basado en respuestas de postlarvas de camarones

    Energy Technology Data Exchange (ETDEWEB)

    Evangelista, A.J.A.; Nascimento, I.A.; Pereira, S.A.; Lopes, M.B.N.L.; Martins, L.K.P. [Laboratorio de Bio Marinha e Biomonitoramento IBIO-UFBA, Campus Universitario de Ondina, Salvador (Brazil); Fillmann, G [Plymouth Marine Laboratory, West Hoe, PL (United Kingdom)

    2005-03-15

    In this study we tested the toxicity of bulk sediment from the northeastern area of Todos os Santos Bay, Bahia, Brazil, to evaluate environmental impact induced by 50 years of exposure to the local petroleum industry (Petrobras). Sediment samples were collected during one year, at three-month intervals, from four sites in areas of oil extraction (Ilha das Fontes, station 4), transportation (Ilhas de Madre de Deus and Pati, stations 2 and 3) and refinement (RELAM, station1). Two reference stations (5 and 6) were located outside the petroleum influence area, to the south of the bay. Static bioassays were conducted for 96 h, using 7-8 day old Litopenaeus vannamei postlarvae (PL). The assays were conducted in 2.5 L plastic jars containing 200 g of surface (1 cm deep) bulk sediment covered by 2 L of dilution water (filtered seawater, 28 ppt salinity, 27 + 2 degree centigrade and DO under saturation). Fifteen exposed PL in each jar were fed daily on 60 recently hatched Artemia salina nauplii. Physico-chemical parameters were monitored. Mortality and dry weight gain were taken as end-points. The PL mortality data obtained for sediment from the Petrobras stations in comparison to the data from the reference stations were not significantly different (P > 0.05); however, the dry weight gain showed significant differences among stations. A maximum value was reached at station 5 (reference area) and a minimum at station 1 (RELAM refinery). Stations 2 and 3 in petroleum transportation areas did not show significant differences (P > 0.05). To evaluate the sensitivity of this bulk-sediment test in detecting contaminant effects generated by the petroleum industry, the toxicity data were considered in terms of the hydrocarbon levels analyzed in sediments from the same Petrobras areas and in one of the control areas, located outside the bay. The results support the assumption that the bulk-sediment bioassay on penaeid PL is a suitable methodology not only to distinguish between

  8. Influência do aquecimento sobre diferentes métodos de titulação de SO2 residual em camarões Litopenaeus vannamei (Boone, 1931 = Influence of heating under different titration methods of residual SO2 on shrimps Litopenaeus vannamei (Boone, 1931

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    Karla Patrícia Brito de Araújo Vieira

    2008-01-01

    Full Text Available O metabissulfito de sódio é o conservante mais aplicado na carcinicultura, com uso amparado em legislações vigentes no país, todavia, seu resíduo, dióxido de enxofre (SO2, em concentrações altas, pode provocar reações adversas à saúde. Este estudo foidesenvolvido com a finalidade de observar a influência do aquecimento sob diferentes métodos de titulação de SO2 residual em camarões Litopenaeus vannamei. Os camarões foram submetidos a nove concentrações de metabissulfito de sódio e avaliados com diferentesmétodos de detecção de SO2. O método da titulação Adolfo Lutz adaptado apresentou grande sensibilidade para detecção de SO2 residual. O método da titulação iodométrica com aquecimento possibilitou a detecção de maiores níveis de SO2, quando comparado aométodo sem o aquecimento. O aquecimento interferiu negativamente no método da fita reativa. Conclui-se que o aquecimento influenciou positivamente a titulação iodométrica, não ocorrendo o mesmo com a fita reativa.Sodium metabisulfite is the most applied preservative in shrimp culture, with its use supported by current legislation; however, its residue, sulfur dioxide (SO2, in high concentrations can provoke adverse health reactions. This study evaluated the influence of heating under different methods of residual SO2 titration in Litopenaeus vannamei. Shrimps were treated with nine concentrations of sodium metabisulfite and evaluated by different methods of SO2 titration. The adapted Adolfo Lutz titration method presented the highest sensitivity for the detection of SO2. The method of iodometric titration with heating made possible the detention ofhigher levels of SO2, when compared to the same method without heating. The heating influenced negatively on the reactive ribbon method. It is concluded that the heating influenced positively on the iodometric titration, as opposed to the reactive ribbon.

  9. Padrão de deslocamento do camarão marinho Litopenaeus vannamei (Boone (Crustacea, Decapoda, Penaeidae nas fases clara e escura ao longo de 24 horas Displacement pattern of marine shrimp Litopenaeus vannamei (Boone, 1931 (Crustacea, Decapoda, Penaeidae in light and dark phases during a 24-hour period

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    Cibele S. Pontes

    2006-03-01

    Full Text Available O padrão diário de atividade de Litopenaeus vannamei (Boone, 1931 (Crustacea, Decapoda, Penaeidae é ainda pouco entendido. Com o objetivo de fornecer subsídios para otimizar o manejo nas fazendas de cultivo através do estudo do comportamento deste animal, foram desenvolvidos experimentos com juvenis (7,57 ± 1,01 g, mantidos em aquários (30 l. Para registro da atividade, os aquários foram submetidos a fotoperíodo artificial (fase do claro e do escuro, 12h:12 h, sendo monitorados 16 camarões (33 m² em cada fase, durante 20 dias, em janelas de 15 min a cada hora. A alimentação foi fornecida em horários aleatórios, sendo registrados os deslocamentos horizontal e vertical (focal contínuo e os comportamentos de natação e exploração (focal instantâneo. Os deslocamentos horizontal e vertical dos camarões foram mais acentuados na fase escura, predominando o comportamento de natação. A exploração do substrato ocorreu em ambas fases, acentuando-se entre cinco e sete horas após o acendimento das luzes.Litopenaeus vannamei (Boone, 1931 daily behavior pattern is still poorly understood. With the aim of optimizing the management of shrimp farms through the study of this animal behavior, experiments were performed with juvenile (7.57 ± 1.01, which were marked and kept in aquariums (30 l. To record activity, the aquariums were submitted to an artificial photoperiod (light and dark phases, 12h:12 h, with 16 shrimp (33 m² monitored in each phase for 20 days, for 15-minute periods every hour. Food was provided at random times and a record kept of horizontal and vertical displacement (continuous focal, swimming and exploration behavior (instantaneous focal. The horizontal and vertical displacements of the shrimp were greater during the dark phase, with a predominance of the swimming behavior. Exploration of the substrate occurred in both phases, with a peak between five and seven hours after the lights were turned on.

  10. Primer registro de la utilización de harinas de Salicornia bigelovii y Scomber japonicus en dietas prácticas para el cultivo súper-intensivo de camarón Litopenaeus stylirostris First record on the use of Salicornia bigelovii and Scomber japonicus fishmeals as feed for Litopenaeus stylirostris under super-intensive farming

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    Manuel de J . Acosta-Ruiz

    2011-11-01

    Full Text Available Se evaluó el efecto de las harinas de Salicornia bigelovii (SA y Scomber japonicus, semiprocesada (HPS como ingredientes en la formulación de dietas para camarón azul Litopenaeus stylirostris, en cultivo súper-intensivo. Se formularon tres diferentes dietas isoproteicas (40% e isocalóricas (6 kcal g-1: (DSA, (DHPS, basal (DBA y una dieta control (DCO. El peso obtenido con DSA y DHPS (0,9 ± 0,014 y 0,8 ± 0,015 g fue similar a la dieta comercial DCO (0,9 ± 0,07 g, no existieron diferencias significativas (P > 0,05 en talla (cm, peso (g y factor de conversión alimenticio (FCA. Los resultados obtenidos sugieren que DSA y DHPS son factibles de utilizar en la formulación de dietas para camarón por ser ingredientes de bajo costo que pueden sustituir a la harina de maíz y pescado tradicional, respectivamente sin efectos detrimentales en el crecimiento y supervivencia.The effect of semi-processed fishmeals (HPS made using Salicornia bigelovii (SA and Scomber japonicusas ingredients in the formulation of diets for blue shrimp Litopenaeus stylirostris undersuper-intensive farming was evaluated. Three different isoproteinic (40% and isocaloric (6 kcal g-1 diets were formulated: DSA, DHPS, basal (DBA; a fourth control diet (DCO was also used. The weights obtained with DSA and DHPS (0.9 ± 0.014 and 0.8 ± 0.015 g were similar to those obtained with the commercial diet (DCO; 0.9 ± 0.07 g, and no significant differences (P > 0.05 were found for size (cm, weight (g, and the feed conversion ratio (FCR. The results suggest that the use of DSA and DHPS in the formulation of diets for shrimp is feasibleas these are low-cost ingredients that can be used as substitutes for cornstarch and traditional fishmeal, respectively, without detrimental effects for shrimp growth and survival.

  11. Utilização de modelos estatísticos para avaliar dados de produção do camarão Litopenaeus vannamei cultivados em águas oligohalina e salgada = Use of statistical models to evaluate production data of Litopenaeus vannamei, reared in oligohaline and salty waters

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    Diogo Bessa Neves Spanghero

    2008-10-01

    Full Text Available O camarão marinho Litopenaeus vannamei pode ser cultivado em águas comampla faixa de salinidade (0,5-60 g L-1. Desta forma, objetivou-se comparar dados de produção desse crustáceo quando cultivado em águas oligohalina (0,5-5,0 g L-1 ou salgada (25,0-40,0 g L-1, utilizando-se modelos matemáticos. Para relacionar as variáveis de manejo com as de produção, os modelos foram formulados com base em um banco de dados com 278 cultivos comerciais da região Nordeste do Brasil. Para estimar os parâmetros dos modelos, utilizou-se a técnica dos mínimos quadrados. A seleção das variáveis foi realizada com o processo de Stepwise associado à transformação de Box e Cox. A adequação das equações e os pressupostos de normalidade, para os erros, foram analisados com base na análise de variância, na estatística de Durbin-Watson, na análise de resíduo e no teste denormalidade. Em todas as equações formuladas, a variável cultivo em água salgada (CAS foi selecionada, evidenciando diferença significativa (p The marine shrimp Litopenaeus vannamei may be reared in a wide range of salinity (0.5-60.0 g L-1. In this study, the production data of shrimp reared either in oligohaline (0.5-5.0 g L-1 or salty waters (25.0- 40.0 g L-1 were compared using mathematical models. The models considered variables related to management and production, and were formulated using a database with 278 culture cycles from different shrimp farms in Northeastern Brazil. The least-squarestechnique was applied to estimate the model’s parameters. The selection of variables used the Stepwise process associated to Box and Cox’s transformation. The adequacy of the equations and the normality estimated for the errors were analyzed on the basis of theanalysis of variance in the Durbin-Watson statistics, on the residue analysis and the normality test. For all the formulated equations, the variable ‘culture in salty water’ was selected, evidencing significant

  12. Seleção de variáveis em modelos matemáticos dos parâmetros de cultivo do camarão marinho Litopenaeus vannamei Selection of variables in mathematical models of culture parameters of marine shrimp Litopenaeus vannamei

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    Ady Marinho Bezerra

    2007-03-01

    Full Text Available O objetivo deste trabalho foi selecionar as variáveis de manejo do camarão marinho Litopenaeus vannamei que mais influenciaram nas variáveis-respostas ao cultivo (produção, produtividade, peso final e taxa de sobrevivência, em modelos matemáticos. O banco de dados foi composto por 83 cultivos, realizados no período de 2003 a 2005, obtidos de uma fazenda comercial localizada no litoral sul de Pernambuco. Para estimar os parâmetros dos modelos, utilizou-se a técnica dos mínimos quadrados. A seleção das variáveis foi realizada com o processo "backward elimination" associado ao método de transformação de Box e Cox. A adequação das equações e os pressupostos de normalidade e homocedasticidade, para os erros, foram analisadas com base na análise de variância e análise de resíduo. É possível relacionar essas variáveis e estabelecer predições com as equações.The objective of this work was to select management variables of the marine shrimp Litopenaeus vannamei that most influenced culture variable responses (production, productivity, final weight and survival rate, in mathematical models. The database was composed of 83 cultures in the period of 2003 to 2005, obtained from a shrimp farm located in the South coast of Pernambuco. To estimate the parameters of the models it was used the technique of least square. The selection of variable was carried through the backward elimination process associated to the Box and Cox transformation. The adequacy of the equations and the hypothesis of normality and homogeneous variance for the errors were analyzed based on the analysis of variance and on the analysis of residuals. It is possible to correlate those variables and to establish predictions with the equations.

  13. Comportamento de Litopenaeus vannamei (Boone (Crustacea, Decapoda, Penaeidae em função da oferta do alimento artificial nas fases clara e escura do período de 24 horas Behavior of Litopenaeus vannamei (Boone (Crustacea, Decapoda, Penaeidae in relation to artificial food offer along light and dark phases in a 24 h period

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    Cibele S. Pontes

    2005-09-01

    Full Text Available A escassez de dados acerca do comportamento do camarão marinho Litopenaeus vannamei (Boone, 1931 relacionado ao alimento artificial ofertado em comedouros poderá induzir a uma alimentação inadequada, aumentando a relação custo/benefício e os impactos ambientais potenciais do seu cultivo. Objetivando fornecer subsídios para a melhoria do manejo alimentar praticado nas fazendas, foram desenvolvidos estudos comportamentais utilizando 64 juvenis de L. vannamei (7,57 ± 1,01 g, submetidos a fotoperíodo artificial, metade deles em ciclo invertido, para observação das suas atividades durante as fases clara e escura. A ração foi oferecida em intervalos pré-estabelecidos, registrando-se antes e depois da oferta: exploração, natação, alimentação e inatividade (focal instantâneo. A natação ocorreu preferencialmente durante a fase escura. A alimentação foi mais elevada na primeira meia hora posterior à oferta, com ênfase nos horários da fase clara. O alimento artificial atuou, em ambas as fases, como um indutor da exploração do substrato, tornando os camarões mais ativos em todos os horários posteriores à sua oferta.The few data on the marine shrimp Litopenaeus vannamei (Boone, 1931 behavior related to artificial food offer in feeding trays may result an inadequate feeding by the animal, increasing the cost/benefit relation in shrimp culture and its potential environmental impact. In order to provide tools for optimizing feeding management in shrimp farms, a behavioral study was developed, using 64 L. vannamei juveniles (7,57 ± 1,01 g. They were submitted to artificial photoperiods, half of them in reversed cycle, in order to register their behavior during light and dark phases. In established intervals, the following activities were registered before and after food exposition: exploration, swimming, feeding and inactivity (instantaneous sampling. Swimming occurred mostly during the dark phase. Feeding time was higher in

  14. Efeito da adição do melaço na relação carbono/nitrogênio no cultivo de camarão Litopenaeus vannamei na fase berçário = Effect of molasses addition on carbon/nitrogen ratio in the nursery phase of Litopenaeus vannamei shrimp culture

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    Ugo Lima Silva

    2009-10-01

    Full Text Available Investigou-se o efeito da adição do melaço nas relações carbono:nitrogênio (C:N sobre o desempenho zootécnico do camarão Litopenaeus vannamei, na fase berçário, quando cultivado sem renovação de água. As relações do C:N foram avaliadas nas proporções de 25:1 (25M, 15:1 (15M e o controle (0M, sem aplicação de carbono, em delineamento experimental inteiramente casualizado, com quatro repetições. Pós-larvas (PL com peso inicial de 2,5 ± 0,5 mg, foram estocadas em 12 tanques (800 L volume útil, em densidades de 6,25 PL L-1, durante 42 dias de cultivo. Ao final do cultivo, os pesos finais dos camarões dos tratamentos 25M (532,0 mg e 15M (540,0 mg foram superiores (p The effect of molasses addiction on carbon:nitrogen ratios (C:N on the performance of Litopenaeus vannamei shrimp during the nursery phase cultured without water exchange was investigated. The C:N ratios were evaluated in 25:1 (25M and 15:1 (15M proportion and a control (0M, with no carbon source addition, in a randomized experimental design with four replicates. Post-larvae (PL with initial weight of 2.5 ± 0.5 mg were stocked in 12 tanks (800 L net volume, at the density of 6.25 PL L-1 during 42 culture days. At the end of culture, the shrimps weights on 25M (532.0 mg and 15M (540.0 mg treatments were higher (p < 0.05 than the 0M (428.6 mg treatment. Shrimp survival was high in all treatments (77.9 to 90.0%, but without significant difference (p ≥ 0.05. Therefore, the 15 and 25:1 C:N ratios showed an increased growth performance of nursery phase L. vannamei post-larvae cultured without water exchange.

  15. Presença de anticorpos da classe IgM de Leptospira interrogans em animais silvestres do Estado do Tocantins, 2002 Presence of IgM antibodies for Leptospira interrogans in wild animals from Tocantins State, 2002

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    Milton Formiga de Souza Júnior

    2006-06-01

    Full Text Available Quatrocentos e vinte e sete amostras de soro provenientes de animais silvestres foram testadas frente a 18 sorovariedades de Leptospira interrogans. De 286 amostras de Cebus apella, 46 (16,1% foram positivas para as sorovariedades pomona, brasiliensis, mini, swajizak, grippothyphosa, sarmin, fluminense, autumnalis, hebdomadis, guaratuba, javanica e icterohaemorhagiae. Das 82 de Alouatta caraya, 2 (2,4% foram positivas para as sorovariedades mangus e fluminense. Das 31 de Nasua nasua, 4 (12,9% foram positivas para as sorovariedades fluminense e javanica. Das 10 amostras de Cerdocyon thous, 2 (20% foram positivas para as sorovariedades fluminense e brasiliensis. Sete de Dasyprocta sp, 6 de Tamandua tetradactila e 5 de Euphractus sexcintus não apresentaram reatividade.Four hundred and twenty-seven serum samples of wild animals were tested against 18 serovars of Leptospira interrogans. Of 286 samples of Cebus apella, 46 (16.1% were positive for the serovars pomona, brasiliensis, mini, swajizak, grippotyphosa, sarmin, fluminense, autumnalis, hebdomadis, guaratuba, javanica and icterohaemorrhagiae. Of 82 samples of Alouatta caraya, 2 (2.4% were positive for the serovars mangus and fluminense. Of 31 samples of Nasua nasua, 4 (12.9% were positive for the serovars fluminense and javanica, and of 10 samples of Cerdocyon thous, 2 (20 % were positive for the serovars fluminense and brasiliensis. Seven samples of Dasyprocta sp, 6 of Tamandua tetradactyla and 5 of Euphractus sexcintus did not present reactivity.

  16. Migraciones en el chaco semiárido de salta: su relación con la ganadería, la explotación forestal y el uso de la fauna silvestre en el departamento rivadavia

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    F. Barbar\\u00E1n

    2001-01-01

    Full Text Available Considerando que la Colonia Rivadavia se fundó en 1862, con el objetivo principal de aprovechar los pastizales del Chaco Semiárido para exportar ganado al Norte de Chile, analizamos desde una perspectiva histórica y demográfica, la relación existente entre las migraciones humanas y las principales actividades económicas del Departamento Rivadavia. Usando el método residual, se calcularon los saldos migratorios entre los censos nacionales de población y vivienda de 1914, 1947, 1960, 1970, 1980, 1991 y la población proyectada a 1998, resultando todos negativos. La expulsión constante de población, obedece al retroceso de la actividad ganadera por sobrepastoreo, lo que condujo a la degradación del ecosistema y la caída del ingreso, induciendo a los pobladores locales a presionar mas sobre el bosque y la fauna silvestre. La pobreza, consecuencia directa de un problema ambiental, obligó a emigrar de Rivadavia. Esta conclusión se sustenta en la evolución de distintos indicadores socio-económicos que se comparan para distintos períodos de los últimos treinta años: necesidades básicas insatisfechas, desnutrición infantil y el fracaso de distintos proyectos de desarrollo dirigidos principalmente a aborígenes en el período 1969 – 1992

  17. Contribución de Graells a la posición de España en el primer convenio internacional para la protección de ciertas especies de la fauna silvestre (1902

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    Ferrero-García, J. J.

    2012-12-01

    Full Text Available The process followed by Spain to decide whether it should subscribe the International Convention for the Protection of Birds Useful to Agriculture, signed in 1902 is described. The fundamental role played by Mariano de la Paz Graells, one of the foremost Spanish naturalists of the 19th century, in the technical review and acceptance of the draft agreement, drawn up in 1895, is highlighted. Furthermore, a critical analysis of Graells’s position in relation to this so-called first international convention for the conservation of certain wildlife species, has been carried out.

    Se describe el proceso que siguió España para decidir si debía suscribir el Convenio Internacional para la Protección de las Aves Útiles a la Agricultura, firmado en 1902. Se pone de manifiesto el papel fundamental que tuvo Mariano de la Paz Graells, uno de los más importantes naturalistas españoles del siglo XIX, en el examen técnico y aceptación del proyecto de dicho convenio, redactado en 1895. Además, se ha realizado un análisis crítico de la postura de Graells en relación con la que puede calificarse como la primera convención internacional para la conservación de ciertas especies de la fauna silvestre.

  18. Differential regulation of hepatopancreatic vitellogenin (VTG) gene expression by two putative molt-inhibiting hormones (MIH1/2) in Pacific white shrimp (Litopenaeus vannamei).

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    Luo, Xing; Chen, Ting; Zhong, Ming; Jiang, Xiao;