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Sample records for camarones silvestres litopenaeus

  1. Uso de diferentes fármacos para anestesiar camarones Litopenaeus vannamei Boone en prácticas de acuacultura - The use of different drugs to anesthetize shrimp Litopenaeus vannamei Boone in aquaculture practices

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    Guzmán-Sáenz, Francisco M

    2010-03-01

    Full Text Available ResumenLas prácticas rutinarias en camarón como la ablación ocular, inseminación artificial, toma de muestra de hemolinfa, colocación de sistemas de identificación (elastómeros, así como algunos manejos para investigación y transporte se dificultan, producen estrés y riesgo de muerte afectando el bienestar. El objetivo del presente trabajo fue determinar por primera vez el efecto anestésico del Hidrocloruro de lidocaína y el Halothano en el camarónLitopenaeus vannamei como medio para facilitar la práctica médica y el manejo de rutina. Se utilizaron camarones Litopenaeus vannamei de 15 a 20 g de peso, en agua marina con parámetros fisicoquímicos controlados. Se realizaron las pruebas en contenedores con 40 L, uno para cada lote de 5 camarones, probando por separado Hidrocloruro de lidocaína y Halothano a diferentes concentraciones en inmersión, midiendo el tiempo de sedación y anestesia general (AG a diferentes dosis. Para verificar el estado anestésico se utilizaroncomo parámetros, el nado, movimientos, respuesta a la agitación del agua y al contacto físico. Al detectar el estado de anestesia general, se procedió a realizar ablaciones oculares, inseminación artificial, toma de muestra de hemolinfa y colocación de elastómeros. Después los camarones se regresaron a sus contenedores originales con una aireación continua y recambio de agua para establecer el tiempo de recuperación. En base a los resultados, se concluye queel anestesiar camarones con las sustancias propuestas, permite realizar los manejos anteriormente señalados con mayor facilidad y menor riesgo de daño o muerte de los mismos mejorando el bienestar animal. SummaryRoutine experiments on shrimp, such as ocular ablation, artificial insemination, haemolymph sampling, placement of identification systems (elastomeres, and some investigation and transport handling, may become difficult, create stress and a high death risk for the animal, affecting its

  2. Uso de diferentes fármacos para anestesiar camarones Litopenaeus vannamei Boone en prácticas de acuacultura - The use of different drugs to anesthetize shrimp Litopenaeus vannamei Boone in aquaculture practices

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    Francisco M. Guzmán-Sáenz; González-Alanís, Pablo; Sanchez Martínez, Jesús G; Gutierrez Salazar, Gilberto; Aguirre Guzmán, Gabriel.; Perez-Castañeda, Roberto

    2010-01-01

    ResumenLas prácticas rutinarias en camarón como la ablación ocular, inseminación artificial, toma de muestra de hemolinfa, colocación de sistemas de identificación (elastómeros), así como algunos manejos para investigación y transporte se dificultan, producen estrés y riesgo de muerte afectando el bienestar. El objetivo del presente trabajo fue determinar por primera vez el efecto anestésico del Hidrocloruro de lidocaína y el Halothano en el camarónLitopenaeus vannamei como medio para facilit...

  3. Control de la reproducción de camarones marinos

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    Jorge Alfaro Montoya

    2016-03-01

    Full Text Available El entendimiento de la biología reproductiva básica es necesario para el mejoramiento de la producción de gametos de camarones. Esta presentación revisará nuestro conocimiento actual en materia de determinación sexual, control hormonal de la maduración, fertilización, y técnicas para la producción comercial de gametos y desove. La maduración controlada de camarones es una actividad comercial; sin embargo, no se conoce el modelo genético de los sexos, ni se ha desarrollado la reversión de sexos y producción de monosexos. La fertilización in vitro de Penaeus es aun una técnica de bajos rendimientos, y la criopreservación de embriones o larvas no existe. La ablación ocular continúa siendo la técnica de manejo hormonal para la maduración de hembras, aunque la aplicación de serotonina podría ser una alternativa en el futuro cercano. El avance logrado en producción de espermatóforos y en el entendimiento de los problemas asociados con su calidad, son nuevas contribuciones para el mejoramiento de la reproducción controlada de camarones.

  4. Efecto de Debaryomyces hansenii en la respuesta antioxidante de juveniles de camarón blanco Litopenaeus vannamei

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    María Pacheco M.

    2012-04-01

    Full Text Available Objetivo. Determinar la respuesta antioxidante [actividad de superóxido dismutasa (SOD y catalasa (CAT] así como la cuenta total de hemocitos (CTH y el contenido de proteínas (CP en camarones (Litopenaeus vannamei expuestos a diferentes dosis y cepas de la levadura Debaryomyces hansenii (DH5, DH6, LL1, y un inmunoestimulante comercial (LAM. Materiales y métodos. Las levaduras fueron cultivadas y suministradas diariamente en concentraciones diferentes (104 – 106 UFC/mL directamente a los tanques de cultivo de los camarones (8 ± 0.2 g mientras que LAM fue aplicado una vez a la semana (0.5 mg/L. Los organismos fueron mantenidos bajo condiciones de laboratorio (28°C, 35%, 80% de recambio diario de agua, dieta comercial para camarón ad libitum. Los tratamientos fueron distribuidos por duplicado y los resultados evaluados a los 15 días con un análisis de varianza y una prueba de Tukey. Resultados. Se registró un CTH significativo (p<0.05 en los tratamientos con DH6 y LL1 (106 UFC/mL comparada con el control, mientras que las cepas DH5 y DH6 revelaron un incremento significativo (p<0.05 de CP con la dosis de 104 UFC/mL. Los camarones tratados con LAM incrementaron significativamente (p<0.05 los valores de SOD y CAT. Conclusiones. Los resultados obtenidos demuestran que D. hansenii incrementa la respuesta antioxidante y CTH en camarones.

  5. Variación temporal en la abundancia de postlarvas y juveniles de camarón azul (Litopenaeus stylirostris) y camarón café (Farfantepenaeus californiensis) en el estuario del Río Colorado

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    MS Galindo-Bect; HM Page; RL Petty; JM Hernández-Ayón; EA Aragón-Noriega; H Bustos-Serrano

    2007-01-01

    Se estudió la variación temporal en la abundancia y composición de postlarvas y juveniles de camarones peneidos en uncanal de mareas del estuario del Río Colorado. Se realizaron muestreos cada dos semanas durante flujo y reflujo de mareas demarzo a noviembre de 2000. Las postlarvas del camarón azul Litopenaeus stylirostris estuvieron presentes a partir de mayo, conmayor densidad durante el flujo de marea a principios de junio. A finales de septiembre se observó un pico de menor densidad, yen ...

  6. Efecto de la salinidad sobre la fisiología energética del camarón blanco Litopenaeus vannamei (Boone Effect of salinity on physiological energetics of white shrimp Litopenaeus vannamei (Boone

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    Gustavo Valdez

    2008-08-01

    Full Text Available Se determinó el balance energético en juveniles de Litopenaeus vannamei, aclimatados a la salinidad como hiperosmóticos (20 ups, isosmóticos (26 ups, o hiposmóticos (32 ups. Además, se calculó la razón atómica O:N. La tasa de ingestión, el consumo de oxígeno, la excreción de amonio y el campo de crecimiento fueron afectados significativamente (p 0.05 en los camarones expuestos a las tres salinidades. Para optimizar las condiciones de cultivo de Litopenaeus vannamei se recomienda mantener a los juveniles en la salinidad para la cual es isosmótico (26 ups, evitando el estrés ambiental, lo que desde el punto de vista fisiológico se canaliza en una mayor cantidad de energía hacia el campo de crecimiento.The energy balance was determined with in Litopenaeus vannamei, juveniles acclimated to salinity where they were hyperosmotic (20 ups, isosmotic (26 ups, and hypo-osmotic (32 ups. Also O:N atomic ratio was calculated. The ingestion rate, oxygen consumption, ammonium excretion and scope for growth were affected significantly (p 0.05 in the shrimps exposed to the three salinities. We recommend maintaining Litopenaeus vannamei juveniles at the salinity level which is isosmotic (26 ups, where they are free of environmental stress, these conditions for white shrimp juveniles would enhance production in the cultivation of this species.

  7. Abundancia de juveniles de camarones peneidos comerciales en la zona norte de Laguna Madre, México Abundance of the commercial penaeid shrimp juveniles in the North zone of Laguna Madre, Mexico

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    Armando T. Wakida-Kusunoki

    2008-03-01

    Full Text Available Se tomaron muestras mensuales de agosto del 2005 a noviembre del 2006, para analizar los cambios de abundancia de juveniles de camarones peneidos comerciales en la parte norte de Laguna Madre, Tamaulipas. En cada una de las nueve estaciones de muestreo se realizó un arrastre nocturno, utilizando una red tipo chinchorro, de tamaño de malla de 1.25 cm y seis metros de longitud de relinga inferior. Se observaron tres especies de peneidos comerciales y cada una de ellas tuvo sus valores más altos de abundancia en diferentes periodos del año. El camarón café, Farfantepenaeus aztecus fue más abundante de abril-mayo; el camarón rosado, Farfantepenaeus dourarum en diciembre-enero y el camarón blanco, Litopenaeus setiferus en julio y septiembre. Estos resultados muestran que estas especies tienen un uso temporal diferencial en Laguna Madre, Tamaulipas.Monthly samplings were carried out during August, 2005 to November, 2006 to analizing the changes of the abundance of commercial juvenile penaeid shrimps in the North part of Laguna Madre, Tamaulipas. In every nine points a night trawl were carried out using a seine net of mesh size of 1.25 cm and 6 m length of bottom rope. Three commercial penaeid shrimp species were found and each one presented high abundance values for different periods of the year. Brown shrimp, Farfantepenaeus aztecus, in April-May period; pink shrimp, Farfantepenaeus dourarum, in December-January, and the white shrimp, Litopenaeus setiferus in July and September. These results showed that different species presented a differential temporal use in Laguna Madre.

  8. Respuesta inmunomoduladora de la superóxido dismutasa en juveniles de camarón blanco (Litopenaeus vannamei expuestos a inmunoestimulantes

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    2005-01-01

    Full Text Available Se estudió la actividad inmunomoduladora de la manganeso superóxido dismutasa (MnSOD en juveniles de camarón blanco (Litopenaeus vannamei expuestos a diferentes inmunoestimulantes. Organismos cuyo peso varió entre 0.7 y 1.0 g fueron inmersos durante 6 h en soluciones de β-glucano, lipopolisacárido (LPS, fucoidán, y Vibrio penaeicida muerto por calor. Se determinó la actividad enzimática de la MnSOD en los organismos para evaluar si los diferentes inmunoestimulantes utilizados eran capaces de inducir actividad antioxidante. Los inmunoestimulantes probados activaron el sistema inmune de los camarones, mostrando un incremento generalizado en la respuesta antioxidante 48 h después del ensayo. El mayor incremento en la actividad de la enzima (3.2 veces superior al grupo control se registró cuando se utilizó la bacterina. Se expusieron los camarones a V. penaeicida 10 días después de la exposición a los inmunoestimulantes, obteniendo un incremento en la actividad antioxidante de los camarones expuestos previamente a β-glucano, LPS y fucoidán. La respuesta enzimática más alta se obtuvo con los organismos estimulados con β-glucano (2.5 veces superior al control. Este estudio demostró la capacidad de los juveniles de camarón para mejorar la respuesta antioxidante después de ser expuestos a inmunoestimulantes y a una bacteria patógena.

  9. Prevalencia y distribución de los principales agentes etiológicos que afectan los langostinos silvestres en Tumbes, Perú

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    Rubén Alfaro Aguilera; Mervin Guevara Torres; Isaías Gonzales Chávez

    2011-01-01

    Se determinó la prevalencia y distribución de diferentes agentes patógenos en langostinos silvestres, en la zona de esteros de la Región Tumbes - Perú, entre marzo y diciembre de 2009. Los canales de marea considerados en este estudio fueron: Boca del Río Tumbes, El Alcalde, Jelí, El Bendito, Envidia, Soledad y Algarrobo. Se colectó un total de 1926 langostinos entre juveniles y pre-adultos de las especies Litopenaeus vannamei, L. stylirostris y Farfantepenaeus californiensis. Utilizando la t...

  10. Effect of hydrosoluble polysaccharides of Macrocystis pyrifera on physiological and metabolic responses of Litopenaeus vannamei infected with Vibrio campbellii Efecto de polisacáridos hidrosolubles de Macrocystis pyrifera sobre las respuestas fisiológicas y metabólicas de Litopenaeus vannamei infectado con Vibrio campbellii

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    Liliana Noemi Sánchez Campos

    2010-01-01

    Full Text Available Adult white shrimp Litopenaeus vannamei between 23 and 32 g of wet weight were injected or submerged in a hydrosoluble polysaccharides extract from Macrocystis pyrifera and infected with Vibrio campbellii. The infection decreased the oxygen consumption rate to 24 mg O2 h-1 kg-1 w.w., in shrimps of the control group, which were only infected with V. campbellii. Immunestimulated shrimps did not decrease their oxygen consumption rate at any hour p.i. (46 mg O2 h-1 kg-1 w.w. maintaining it similar to the pre-injection group. Glucose level in the hemolymph of V. campbellii infected shrimps at two hours p.i. was significantly higher (p > 0.05 than the level of the pre-injection group. L. vannamei injected with the extract showed a significant decrease (p > 0.05 in their glucose level at 12 hours p.i., but at 24 hours p.i. it returned to normal level. Shrimps submerged in the extract showed no significant glucose level difference (p 0.05 immunoestimulated shrimps showed an increase in their total proteins levels. This study concluded that administration of extract of M. pyrifera via injection and immersion in adult white shrimp can be used for immunostimulation purposes.Adultos de camarón blanco Litopenaeus vannamei de entre 23 y 32 g de peso húmedo fueron inyectados o sumergidos en un extracto de polisacáridos hidrosolubles de Macrocystis pyrifera e infectados con Vibrio campbellii. La infección disminuyó la tasa de consumo de oxígeno a 24 mg O2 h-1 kg-1 p.h. en los camarones del grupo control infectados con V. campbellii. Los camarones inmunoestimulados vía inyección e inmersión, mantuvieron una tasa de consumo de oxígeno de 46 mg O2 h-1 kg-1 p.h., similar a los camarones control. La concentración de glucosa en la hemolinfa de los adultos infectados con V. campbellii a las dos horas p.i. fue significativamente más alto (p > 0.05 que la del grupo control de pre-inyección. Los inyectados con el extracto e infectados con V. campbellii

  11. Análisis proteomico de la subunidad LSL1 de la lectina del Camaron Blanco del Golfo de Mexico Litopenaeus setiferus

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    Alpuche-Osorno, Ja,b.Pereyra, Ma.,Vazquez, Lc., Agundis, Ca., Rosas, Cd., Zenteno, Ea. a

    2005-02-01

    Full Text Available Se purificó por filtración en Sephacryl S-300 una subunidad de 84 kDa de la lectina de L. setiferus (LsL1, específica para azucares N-acetilados. Por dicroísmo circular se encontró que LsL1 contiene en su estructura secundaria 49% de hojas plegadas beta y 6.9% de alfa hélices. La secuencia de aminoácidos de la subunidad LsL1 se determinó por MALDI-TOF a partir de péptidos trípticos. Comparando los valores con la base de datos NCBInr (Swiss-Prot 2003/08/06, esta subunidad mostró 23% de similitud con el precursor hemocianina, proteína responsable de la respiración en crustáceos, que a su vez ha sido relacionada con la generación de péptidos de defensa; al alinear el péptido con otras proteínas involucradas con la defensa en crustáceos muestran una similitud del 80% con hemocianina, y profenoloxidasa, por lo que podemos asumir que las lectinas de esta especie participan en procesos de defensa. By filtration in a column of Sephacryll S-300 was purified a subunit of 84 kDa of the lectina of L. setiferus (LsL1, specific for N-acetilated sugars. By Circular dicroism we found that LsL1 in its secondary structure contains 49% of sheet beta and 6.9% of alfa helix. The sequence of amino acids of the subunit LsL1 were assayed from triptic peptides by MALDI-TOF and they had a rank of mass from 768.36 to 3379.76 m/z. Comparing these data with relative values obtained of the database NCBInr (Swiss-Prot 2003/08/06, this protein showed 23% of homology with a precursor of the responsible protein for the respiration in crustaceans, the one that besides has related to the generation of defense peptides. Alignments with other proteins related to mechanisms of defense in crustaceans indicate a homology of the 80% with similar proteins to the hemocyanin, and the profenoloxidase. We can assume that functionally lectinas of this species participate actively in processes of defense.

  12. Respuestas Bioquímicas de Camarones Marinos a Factores Ambientales

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    Juan Alpuche;

    2005-05-01

    Full Text Available Las modificaciones constantes en el medio ambiente marino y los sistemas costeros son ocasionados tanto por desastres naturales como huracanes, terremotos, inundaciones, así como por actividades humanas. En la mayoría de los organismos marinos, las variables ambientales son determinantes de los cambios fisiológicos. Ante alguna modificación en el ambiente ocurren cambios bioquímicos en los organismos que les permiten mantener la homeostasis con su hábitat.El presente trabajo, es una recopilación bibliográfica de las respuestas a variaciones ambientales en peneidos. Se revisaron los cambios generados por variaciones en la temperatura, la geología de las cuencas, contaminantes, salinidad y materia orgánica en el medio marino.Las respuestas bioquímicas de los organismos a cambios químicos en la composición del agua, van desde ajustes en la permeabilidad de las membranas celulares hasta hiperventilación en branquias.Al intervenir la temperatura en los procesos metabólicos de estos organismos, se presentan alteraciones en la regulación de la respuesta inmune, provocando que los mecanismos de defensa se reviertan contra las células propias atacándolas como extrañas.El oxigeno disuelto (OD tiene un papel regulador que está dado por su intervención directa en la capacidad de los organismos para la obtención de energía a partir de la respiración, por la vía de la fosforilación oxidativa.Existen pocos estudios en los cuales se haya evaluado la tolerancia de organismos juveniles o adultos de L. setiferus a contaminantes específicos. Sin embargo, algunos reportes indican que algunas especies resisten más que otras a contaminantes.La salinidad afecta la distribución de animales marinos y estuarinos, por lo cual la distribución de camarones peneidos esta relacionada directamente con gradientes de salinidad formados latitudinalmente.L. setiferus tiene como hábitat un margen geológicamente inactivo, con caracter

  13. Patógenos introducidos al Perú en post larvas de Litopenaeus vannamei importadas

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    Mervin Guevara

    2012-11-01

    Full Text Available El comercio internacional de animales acuáticos es uno de los factores más importantes en la introducción involuntaria de patógenos a una región libre de estos. Los patógenos introducidos han sido causa de enormes pérdidas económicas en organismos bajo cultivo y daños ecológicos en poblaciones silvestres. En este trabajo, utilizando la técnica de la PCR se analizaron postlarvas de Litopenaeus vannamei importadas desde Ecuador para ser utilizada en los cultivos de langostinos en Tumbes. La finalidad fue detectar patógenos como el virus de la mancha blanca (WSV, virus de la cabeza amarilla (YHV, virus de la necrosis hipodérmica y hematopoyética infecciosa (IHHNV, Baculovirus penaei (BP, virus del síndrome de Taura (TSV y la bacteria de la hepatopancreatitis necrotizante (NHPB. Se analizaron 177 muestras de postlarvas en el año 2009 y 274 en el 2010. En el año 2009 se encontraron cuatro patógenos infecciosos en las postlarvas analizadas, siendo más frecuente el IHHNV (9,60%. En el año 2010 la frecuencia de aparición de postlarvas infectadas con IHHNV fue mayor (16,79%. Tanto en el 2009 como en el 2010 se encontraron postlarvas positivas a NHPB, BP y TSV

  14. PRODUCTOS NATURALES COMO ESTIMULADORES DEL SISTEMA INMUNOLÓGICO DE Litopenaeus vannamei, INFECTADO CON Vibrio parahaemolyticus

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    Nelson Pe\\u00F1a-Navarro

    2013-01-01

    Full Text Available El objetivo de este trabajo fue determinar la respuesta inmunológica de Litopenaeus vannamei con manano-oligosacáridos (T1, ajo (T2 y un compuesto de extractos de plantas (T3 después de ser infectados con Vibrio parahaemolyticus. Se realizaron dos bioensayos con una duración de seis (1x106 UFC y catorce (3x106 UFC días en el Centro de Investigación en Ciencias del Mar y Limnología de la Universidad de Costa Rica durante el 2011. Para esta investigación se extrajeron 120 camarones para cada periodo experimental de fincas ubicadas en la Península de Nicoya. Al finalizar la dosificación de los productos se evaluó la ganancia de peso y la conversión alimenticia. Luego de la infección con V. parahaemolyticus se realizaron hemogramas, coagulación, bacteriología de hemolinfa y mortalidad acumulada. Los parámetros inmunológicos no mostraron diferencias estadísticas (P>0,05 entre tratamientos en ningún periodo, sin embargo a los seis días T1 mostró los mejores resultados con 41,07x105 hemocitos/ml; una coagulación de 34,40 s y 4,44x103 UFC/ ml. En el periodo de catorce días T2 obtuvo los mejores valores (55,76x105 hemocitos/ml; una coagulación de 34,20 s y15,4x103 UFC/ml. La mortalidad acumulada se presentó a las diez horas de inoculación, hubo menor cantidad de muertes en T1 y T3 (76,2% a los seis días, mientras que a los catorce días fue para T1 (93,2%. La ganancia de peso y la conversión alimenticia resultaron con diferencias estadísticas (P<0,05 solo para el bioensayo que se extendió por seis días, donde T2 presentó una biomasa de 54,3 g, un incremento en la ganancia de peso de 19,3% y una conversión alimenticia de 1,4.

  15. Respuesta inmune y expresión de genes en el camarón blanco (Litopenaeus vannamei inducida por inmunoestimulantes microbianos

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    Antonio Luna-González

    2013-11-01

    Full Text Available Se realizó un bioensayo de 26 días para evaluar el efecto inmunoestimulante de bacterias ácido lácticas y levaduras (MI, adicionadas en el alimento, en Litopenaeus vannamei. Los tratamientos del bioensayo se realizaron por triplicado: I dieta control (Camaronina®; II MI en alimento, diario; III MI en alimento, cada tres días y; IV MI en alimento, cada seis días. Los camarones sólo eran libres de WSSV. Para el estudio del sistema inmune se hizo un conteo total de hemocitos, se determinó bioquímicamente la concentración de anión superóxido, y la actividad de la fenoloxidasa. También se estudió la expresión semicuantitativa de seis genes del sistema inmune, utilizando la técnica de RT-PCR. No hubo aumento significativo en el crecimiento y la supervivencia, el conteo total de hemocitos, la concentración de la proteína total en plasma y hemocitos, y la concentración del anión superóxido. La actividad de la fenoloxidasa en plasma en el tratamiento IV fue significativamente mayor que en los tratamientos I, II y III. La fenoloxidasa del SLH (proFO en el tratamiento IV fue significativamente mayor que en los tratamientos I y III. La MI provocó una sobreexpresión significativa de los genes que codifican para la profenoloxidasa (tratamiento IV, lisozima (tratamiento III y transglutaminasa (tratamiento II, con respecto a los animales no tratados (control. La mezcla de inmunoestimulantes microbianos puede aumentar la resistencia de L. vannamei contra patógenos en los cultivos.

  16. Control Hormonal de la Fertilidad en Animales Silvestres

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    M P Paredes-Higuera

    2010-01-01

    Las especies silvestres se encuentran en peligro de extinción y se han visto obligadas a tener una vida en cautiverio donde se alteran sus hábitos alimenticios, sociales y las condiciones medioambientales, lo cual afecta el comportamiento reproductivo y la fertilidad. El objetivo principal de la conservación animal es mantener la biodiversidad dado que la remoción o desaparición de una especie puede afectar el funcionamiento del ecosistema global; por esto se ha incrementado el uso de biotecn...

  17. Reproducción de cochinilla silvestre Dactylopius opuntiae (Homóptera: Dactylopiidae)

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    Arnoldo Flores-Hernández; Bernardo Murillo-Amador; José Luis García-Hernández; Enrique Troyo-Diéguez; Edgar Omar Rueda-Puente; José Cruz Salazar-Torres

    2006-01-01

    Por sus características, la cochinilla silvestre ofrece perspectivas de aprovechamiento como fuente de carmín y para el mejoramiento genético de la grana fi na. Por lo anterior, se estableció una multiplicación y crianza de cochinilla silvestre, proveniente de la zona árida del norte de México para caracterizarla, utilizando como hospedero a Opuntia megacantha Salm Dyck. Los resultados indican que la especie silvestre pertenece al género-especie Dactylopius opuntia. Se determinó la presencia ...

  18. Sensory quality of frozen shrimp Litopenaeus vannamei

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    Inês Maria Barbosa Nunes Queiroga

    2014-09-01

    Full Text Available The objective of this research was to evaluate the sensory quality of the marine shrimp Litopenaeus vannamei grown and stored in a freezer. A sensory analysis consisted of the Quality Index Method (MIQ to review the raw shrimp and Descriptive Analysis (AD in cooked shrimp in samples stored for a period of 90 days, using eight previously trained panelists. Accommodation comprising shrimp filet (100-120 pieces / kg samples were subjected to freezing in liquid nitrogen (- 86 °C, Freezing Tunnel (- 35 °C and Domestic Freezer (- 18 °C. At 0, 30, 60 and 90 days of storage in these freezing systems, determination of pH, water holding capacity (WHC, weight loss during cooking (WLC, shearing force, color, total volatile bases (TVB and thiobarbituric acid reactive substances (TBARS were performed. The attributes manual firmness and softness showed better results in rapid freezing. The color parameters (a *, b * and L *, WHC and WCL were higher at 90 days of storage, no significant losses were observed. Highlighted the strength of greater shear in slowly frozen samples at 90 days, confirming the results reported by the sensory panel. At 90 days of storage, the frozen shrimp showed good sensory quality and physical and chemistry characteristics. The shelf life of this shrimp could be set at about 90 days.

  19. Induction of ovarian maturation and spawning by combined treatment of serotonin and a dopamine antagonist, spiperone in Litopenaeus stylirostris and Litopenaeus vannamei

    NARCIS (Netherlands)

    Alfaro Montoya, J.; Zuniga, G.; Komen, J.

    2004-01-01

    The study was designed to develop a reliable technique for inducing ovarian maturation and spawning in Litopenaeus stylirostris and Litopenaeus vannamei, as an alternative to the traditional and destructive eyestalk ablation. Two combinations of molecules were evaluated: (a) serotonin (5-HT) at 50 ¿

  20. COMERCIO DE FAUNA SILVESTRE EN COLOMBIA WILDLIFE TRADE IN COLOMBIA

    Directory of Open Access Journals (Sweden)

    Néstor Javier Mancera Rodríguez

    2008-12-01

    Full Text Available Este trabajo ofrece un panorama sobre las actividades relacionadas con el comercio de bienes derivados de las especies de fauna silvestre en Colombia, abordando el tema desde el desarrollo que ha tenido su actividad productiva, el aprovechamiento extractivo, así como la dinámica de su comercio legal e ilegal en el país y el desarrollo y promoción de alternativas productivas sustentadas en su aprovechamiento. Se analizó la información secundaria de entidades como el Ministerio de Ambiente, Vivienda y Desarrollo Territorial, las Corporaciones Autónomas Regionales y Autoridades Ambientales Urbanas, el Instituto Colombiano de Desarrollo Rural-INCODER, las Autoridades Policiales, los Institutos de Investigación, el Departamento Administrativo Nacional de Estadística, la Dirección de Impuestos y Aduanas Nacionales-DIAN, el Ministerio de Comercio, Industria y Turismo, y PROEXPORT. entre otras. En Colombia, el comercio de especies de fauna silvestre está centrado principalmente en la extracción de ejemplares de forma ilegal, lo cual ha generado desequilibrios en las poblaciones naturales y ha repercutido en el deterioro de la dinámica de los ecosistemas. El comercio legal de fauna silvestre se basa en la producción de unas pocas especies entre las que se destacan la babilla (Caiman crocodilus, el chigüiro (Hydrochaeris hydrochaeris, cerca de 200 especies de peces ornamentales y en menor medida el lobo pollero (Tupinambis nigropunctatus, la iguana (Iguana iguana, la boa (Boa constrictor, escarabajos (Dynastes hercules y mariposas. En el país no se tiene información exacta sobre el número de incautaciones realizadas en los operativos de control al tráfico ilegal de fauna, y no existe un conocimiento de la dinámica de este comercio ilegal.This work offers a current view on the activities related to the trade of derived from the wildlife species in Colombia, approaching the topic from the development that has had its productive activity

  1. Prevalencia y distribución de los principales agentes etiológicos que afectan los langostinos silvestres en Tumbes, Perú

    Directory of Open Access Journals (Sweden)

    Rubén Alfaro Aguilera

    2011-05-01

    Full Text Available Se determinó la prevalencia y distribución de diferentes agentes patógenos en langostinos silvestres, en la zona de esteros de la Región Tumbes - Perú, entre marzo y diciembre de 2009. Los canales de marea considerados en este estudio fueron: Boca del Río Tumbes, El Alcalde, Jelí, El Bendito, Envidia, Soledad y Algarrobo. Se colectó un total de 1926 langostinos entre juveniles y pre-adultos de las especies Litopenaeus vannamei, L. stylirostris y Farfantepenaeus californiensis. Utilizando la técnica de la PCR, se detectó la presencia de los patógenos NHPB (0,62%, IHHNV (0,31%, BP (1,61% y WSV (2,75%; no se encontró infección por TSV. Las tres especies en estudio fueron positivas a WSV y BP, presentándose la mayor prevalencia de infección por WSV (2,98% en la especie L. stylirostris y por BP (2,66% en L. vannamei. La NHPB fue detectada en las especies L. vannamei y L. stylirostris con 0,77% y 0,43% de prevalencia respectivamente. Se obtuvo una prevalencia de 0,52% para IHHNV en L. vannamei. Las más altas prevalencias de las infecciones por WSV, BP, NHPB e IHHNV se registraron en los canales de marea El Alcalde (10,79%, Algarrobo (4,51%, Envidia (2,26% y Jelí (5,05%. Los datos señalan la presencia constante de diversos patógenos virales y bacterianos en diferentes especies de peneidos y su amplia distribución a lo largo del litoral tumbesino, lo que constituye un riesgo potencial para el desarrollo de la acuicultura en la región, y podría afectar las poblaciones naturales de langostinos.

  2. Bioseguridad en el Manejo de Fauna Silvestre y no Convencional

    Directory of Open Access Journals (Sweden)

    Nestor Varela

    2011-06-01

    Full Text Available La bioseguridad es un conjunto de actuaciones para prevenir la pérdida de la integridad biológica a diversas escalas, estando relacionada como concepto a múltiples campos y disciplinas. En las ciencias de la salud, la bioseguridad hace referencia a la prevención de la contaminación derivada del contacto con órganos o tejidos de origen biológico y microorganismos. Los problemas traumáticos, las reacciones alérgicas o irritativas y las zoonosis son las causas de enfermedad más frecuentes en las personas que manejan fauna, por ello la prevención debe dirigirse prioritariamente al control de estos factores. Las medidas de protección incluyen la higiene personal, el aseo, limpieza y desinfección de las instalaciones, elementos y equipos, el empleo de indumentaria de protección personal, y la adopción de protocolos específicos de acuerdo al nivel de riesgo detectado para las diversas actividades realizadas por el personal. Se describe un código de prácticas generales a varios niveles para personas encargadas del manejo de fauna y se describen los niveles de bioseguridad que se emplean como protocolos ante la ausencia de conocimiento sobre bioriesgo durante la práctica con animales silvestres.

  3. Ectoparásitos del orden PHTHIRAPTERA en Aves Silvestres

    Directory of Open Access Journals (Sweden)

    Andrea Saavedra-Orjuela

    2014-12-01

    Full Text Available Los ectoparásitos de las aves silvestres en Colombia han sido poco estudiados. Se tiene conocimiento que éstos animales pueden ser afectados por ácaros, pulgas, garrapatas y moscas. Sin embargo, los piojos juegan un papel protagónico en el ectoparasitismo de aves silvestres y tienden a ser altamente específicos con sus huéspedes; por lo tanto, hay muchas especies de Phthiraptera que se han registrado a partir de un solo huésped, mientras que algunas otras especies se han registrado en varias especies de aves estrechamente relacionadas. Se hace verdaderamente importante desde el ámbito médico veterinario tener registro del tipo de ectoparásito involucrado en caso de haber infestaciones severas. Los factores que favorecen el contacto directo entre las aves infestadas con otras, como el número de veces que esto ocurra, el tiempo de duración de los contactos y la longitud de las plumas, contribuye altamente a la infestación entre individuos. Así mismo, las medidas de los especímenes se relacionan directamente con el estadio de desarrollo en el que se encuentre y éste vivirá todo su ciclo de vida sobre el ave pues es completamente dependiente para su supervivencia. En el orden Phthiraptera se encuentra el suborden Amblycera, de los cuales se conoce que tres familias parasitan a las aves (Laemobothridae, Menoponidae y Ricinidae y el suborden Ischnocera, de la cual sólo una de sus familias principales (Philopteridae parasita a las aves, la cual involucra una numerosa lista de especies.   ECTOPARASITES OF THE PHTHIRAPTERA ORDER IN WILD BIRDS. Abstract. Ectoparasites of wild birds in Colombia have been little studied. It is known these animals can be affected by mites, fleas, ticks and flies. However, lice play a leading role in wild birds ectoparasitism and tend to be highly specific to their guests; therefore, there are many species of Phthiraptera that have been recorded from a single host, while some other species have been

  4. Parasitos de aves e mamíferos silvestres em cativeiro no estado de Pernambuco

    OpenAIRE

    Pauline Marie de Souza Santos; Silvia Gabriela Nunes da Silva; Cristina Farias da Fonseca; Jaqueline Bianque de Oliveira

    2015-01-01

    Resumo: Os animais silvestres são hospedeiros de uma grande variedade de parasitos que podem interferir em sua conservação ex situ. O objetivo deste estudo foi identificar os parasitos gastrointestinais (PGI) e ectoparasitos dos animais do Centro de Triagem de Animais Silvestres (CETAS) do Instituto Brasileiro de Meio Ambiente e Recursos Naturais Renováveis (IBAMA) de Recife, Pernambuco, além de determinar os aspectos do manejo em cativeiro que possam estar relacionados com os parasitos ident...

  5. Estudio de la triquinelosis en la fauna silvestre del noroeste español

    OpenAIRE

    Rodríguez Rodríguez, María Estrella

    2016-01-01

    La triquinelosis en España se considera una zoonosis endémica cuyo ciclo doméstico está aparentemente controlado, pero todos los años aparecen brotes en humana relacionados con el consumo de carne de cerdo y jabalí sin control sanitario. En este sentido, la fauna silvestre actúa de reservorio de Trichinella spp., especialmente el jabalí y los carnívoros. Para conocer la situación epidemiológica actual de Trichinella spp. en la fauna silvestre de Galicia, hemos realizado el estudio más ampl...

  6. Abejas silvestres (Hymenoptera: Apoidea) como bioindicadores en el neotrópico

    OpenAIRE

    Enrique Reyes-Novelo; Virginia Meléndez Ramírez; Hugo Delfín González; Ricardo Ayala

    2009-01-01

    Este trabajo es una revisión sobre el potencial de las abejas silvestres como grupo indicador en estudios de diversidad y fragmentación de hábitat. Se describen los criterios utilizados para seleccionar grupos de organismos como bioindicadores y se discute la información disponible para evaluar si las abejas pueden ser consideradas idóneas. La información disponible sugiere que las abejas silvestres cumplen con los criterios requeridos para considerarlas un grupo indicador y se recomienda su ...

  7. Parásitos de animales silvestres en cautiverio en Lima, Perú

    OpenAIRE

    Lily Arrojo

    2013-01-01

    Los parásitos de los animales silvestres en cautiverio peruanos se registran aquí. La mayoría de las especies son nematodos. Dos especies son al patógeno de host: Prosthenorchois elegans y Strongyloides sp.

  8. Toxoplasmosis Silvestre: relación presa-predador y papel funcional de pequeños felinos silvestres neotropicales en la transmisión -resumen-

    OpenAIRE

    William A Cañón-Franco; N López-Orozco; A F Malheiros; M Santos-Filho; Gennari, S.M.

    2014-01-01

    Pequeños felinos neotropicales se ubican en el tope de la cadena alimenticia y‚ aunque críticamente amenazados‚ ocho especies de felinos son relativamente abundantes en el continente americano‚ sin embargo‚ pocos estudios biológicos sobre la circulación de agentes infecciosos han sido realizados. Para determinar el papel epidemiológico que cumplen las poblaciones de felinos silvestres en la transmisión de la Toxoplasmosis en la región sur de Brasil (estado de Rio Grande do Sul)‚ fue determina...

  9. ZOONOSIS TRANSMITIDAS POR ANIMALES SILVESTRES Y SU IMPACTO EN LAS ENFERMEDADES EMERGENTES Y REEMERGENTES

    Directory of Open Access Journals (Sweden)

    Santiago Monsalve B

    2009-08-01

    Full Text Available Las enfermedades emergentes y reemergentes originadas desde animales de vida silvestre, pueden ser transmitidas a las poblaciones humanas por contacto directo o por vectores. Las zoonosis determinan una gran problemática social epidemiológica. La relación que tiene las enfermedades emergentes y reemergentes está ligada a la pérdida de la biodiversidad por factores antropogénicos, debido a la destrucción de hábitats naturales, el tráfico de fauna y a la pérdida de diversidad genética. Todos estos aspectos juegan un papel en la aparición de las patologías de origen infeccioso. Esta revisión intenta acercarse al conocimiento de las zoonosis transmitidas por animales silvestres y su impacto en las enfermedades emergentes y reemergentes.

  10. Ciutat de Mallorca, 31 de desembre del 1229: Sant Silvestre, Sant Jordi i Jaume I

    OpenAIRE

    Xavier Renedo Puig

    2007-01-01

    Un dels episodis més memorables del Llibre dels feits és la descripció de l’atac final a la Ciutat de Mallorca el 31 de desembre del 1229. Es tracta del dia de sant Silvestre i, encara que Jaume I no esmenti aquesta efemèride en el seu relat, potser no es tracta d’una coincidència. Per un cristià del segle XIII sant Silvestre era, d’acord amb el divulgadíssim Actus Beati Silvestri, el responsable de la conversió al cristianisme de l’emperador Constantí i d’Helena, la seva mare. Era, per tant,...

  11. CARNE DE MONTE Y CONSUMO DE FAUNA SILVESTRE EN LA ORINOQUIA Y AMAZONIA ( COLOMBIA Y VENEZUELA)

    OpenAIRE

    2012-01-01

    Este taller regional estuvo dirigido al tema de carne de monte, consumo de fauna silvestre y procesos alternativos (cría de animales en cautiverio y piscicultura extensiva, entre otros), como elementos que aportan a la seguridad alimentaria de las comunidades locales (rurales e indígenas) en la Orinoquia y Amazonia (Colombia -­ Venezuela). Este evento tuvo como antecedente tres talleres realizados desde 2009 (talleres II, III, IV) y reuniones de expertos llevadas a cabo en 2002 para actual...

  12. [Reseñas Bibliográficas] Atlas de la flora vascular silvestre de Burgos

    Directory of Open Access Journals (Sweden)

    Iñaki Aizpuru

    2005-01-01

    Full Text Available Es una reseña bibliográfica de la obra, Atlas de la flora vascular silvestre de Burgos / Alejandre Sáenz, J.M. García-López, L. Marín Padellano, G. Mateo Sanz, E. Miguélez del coso, C. Molina Martín, G. Montamarta Prieto, S. Patino Sánchez, M.A. Pinto Cebrián 6 J. Valencia Janices.

  13. Toxoplasmosis Silvestre: relación presa-predador y papel funcional de pequeños felinos silvestres neotropicales en la transmisión -resumen-

    Directory of Open Access Journals (Sweden)

    William A Cañón-Franco

    2014-12-01

    Full Text Available Pequeños felinos neotropicales se ubican en el tope de la cadena alimenticia y‚ aunque críticamente amenazados‚ ocho especies de felinos son relativamente abundantes en el continente americano‚ sin embargo‚ pocos estudios biológicos sobre la circulación de agentes infecciosos han sido realizados. Para determinar el papel epidemiológico que cumplen las poblaciones de felinos silvestres en la transmisión de la Toxoplasmosis en la región sur de Brasil (estado de Rio Grande do Sul‚ fue determinada la frecuencia molecular de Toxoplasma gondii. La amplificación del marcador ITS1 fue posible en 31 de los 90 felinos analizados (34‚4%‚ de las especies Puma yagouaroundi (9/22‚ Leopardus geoffroyi (6/22‚ Leopardus tigrinus (8/28‚ Leopardus wiedii (6/10‚ Leopardus pardalis (1/1 y Leopardus colocolo (1/7. Dos nuevos genotipos de T. gondii fueron caracterizados por PCR-RFLP (Lw#31Tn - Py#21Sm y el genotipo Py#56Br previamente descrito en gatos domésticos‚ perros y chigüiros del Brasil. Adicionalmente‚ fue parcialmente caracterizado el genotipo I (Py#36Sm‚ evidenciando la circulación ambiental y su posible relación en la presentación de toxoplasmosis ocular en humanos. Los resultados demuestran que pequeños felinos silvestres tienen un papel importante en la transmisión de la toxoplasmosis en la interfase animales domésticos-silvestres‚ por lo que no se descarta la posible hibridación con genotipos urbanos. La carnivoría y las relaciones presa-predador son fundamentales en la transmisión horizontal de Toxoplasma. Aunque existan variaciones biogeográficas y estacionales en la dieta de los felinos silvestres‚ aproximadamente 40% a 80%‚ corresponde a pequeños mamíferos no voladores (marsupiales y roedores. El estudio de la dinámica de transmisión en diferentes niveles tróficos es de gran interés para la comprensión ecológica del ciclo silvestre de este parásito. Esta propuesta se fundamenta en evaluar

  14. Enfermedad de la mancha del caparazón en el camarón de cultivo Litopenaeus vannamei - Brown spot disease in aquaculture shrimp litopenaeus vanname.

    Directory of Open Access Journals (Sweden)

    Manuel Rubio Limonta

    2012-07-01

    Full Text Available ResumenLa presencia de manchas color café a negro en el camarón Litopenaeus vannamei constituye un síndrome relacionado con infecciones bacterianas o fúngicas en cutícula, apéndices o branquias, causante de depreciación comercial del producto.AbstractThe presence of brown to black spots on shrimp Litopenaeus vannamei is a syndrome related to bacterial or fungal cuticle, appendages or gills, causing commercial depreciation of the product.

  15. Efficacy of marine green alga Ulva fasciata extract on the management of shrimp bacterial diseases Eficacia del extracto del alga marina verde Ulva fasciata sobre el manejo de las enfermedades bacterianas en camarones

    Directory of Open Access Journals (Sweden)

    Joseph Selvin

    2011-07-01

    Full Text Available Secondary metabolites of the green algae, Ulva fasciata, were tested to determine the efficacy of controlling shrimp bacterial pathogens. Exploratory experiments indicated that an intermediate dose (1 g kg-1 of shrimp of Ulva in the diet was highly effective at controlling bacterial pathogens of shrimp, as compared to lower (500 mg kg-1 and higher (1.5 g kg-1 doses. The pilot experiments evaluated the percent of relative protection afforded shrimps treated with Ulva diet and faced with various concentrations of bacterial pathogen. The survival of shrimps treated with Ulva diet was significant (P Metabolites secundario de algas verdes Ulva fasciata fue probado para determinar la eficacia de controlar el camarón pathogens bacterial. Las conclusiones de experimentos exploratorios indicaron que la dosis mediana (1 g kg-1 de camarón de dieta Ulva era sumamente eficaz en el control de pathogens bacterial de camarón cuando comparado al más abajo (500 mg kg-1 y más alto (1,5 g kg-1 dosis. En los experimentos pilotos, la protección de pariente de por ciento de camarones trató con la dieta Ulva y desafió con varias concentraciones de bacterial patógeno fueron evaluados. La supervivencia de camarones trató con la dieta Ulva era significativo (P < 0,01. Basado en las conclusiones presentes, podría ser deducido que U. verde fasciata puede ser una fuente excelente para desarrollar la comida potente medicinal para la dirección de enfermedad de camarón.

  16. Characterization of four hemocyanin isoforms in Litopenaeus vannamei

    Institute of Scientific and Technical Information of China (English)

    XU Jingxiang; RUAN Lingwei; LI Zhen; YU Xiaoman; LI Sedong; SHI Hong; XU Xun

    2015-01-01

    In this study, the gene encoding hemocyanin subunit L, LvHcL, was cloned from Litopenaeus vannamei and the genomic organization was characterized. This gene was diverse with many SNPs and also had at least four isoforms, while one of them (LvHcL4) only had two exons and the exon2 was missed. Transcription analysis showed that these isoforms of LvHcL were up-regulated after WSSV challenge in WSSV-resistant shrimp, while the transcriptions were decreased constantly in WSSV-susceptible shrimp. It is suggested that the hemocyanin had rich polymorphism and was involved in the antiviral response. These results could extend our previous findings and provide insights into the immune feature of hemocyanin, which would be helpful for further studies aimed at antiviral mechanism in inver-tebrate.

  17. Análisis histopatológico en Litopenaeus vannamei infectado con Vibrio parahaemolyticus.

    Directory of Open Access Journals (Sweden)

    Nelson Peña-Navarro

    2015-01-01

    Full Text Available El objetivo de este trabajo fue describir los principales resultados histopatológicos obtenidos de las diversas respuestas inmunológicas y efectos citopáticos en L. vannamei, después de una infección inducida con V. parahaemolyticus. La inoculación bacterial se realizó en el Centro de Investigación en Ciencias del Mar y Limnología de la Universidad de Costa Rica, entre junio y agosto del 2011. Para la investigación se utilizaron 40 camarones extraídos de fincas ubicadas en la Península de Nicoya; a cada uno se le inyectó en promedio 2,0x106 UFC de V. parahaemolyticus. Al finalizar el periodo de infección, se realizaron los análisis histopatológicos en branquias, epitelio subcuticular, hepatopáncreas, intestino, órgano linfoide, tejido hematopoyético, músculo estriado, tejido nervioso, corazón, entre otras. Los principales hallazgos a nivel histológico fueron hemocitos melanizados, infiltración hemocítica, melanosis, esferoides tipo A, B y C, mionecrosis coagulativa, fagocitosis, picnosis, entre otras, útiles para determinar el estado sanitario de los camarones y en particular, el efecto citopático de V. parahaemolyticus en infecciones sistémicas.

  18. Impactes de la fauna silvestre al sector primari : la Vall d'Alinyà

    OpenAIRE

    Montecabra (Grup de recerca); González, Sofia; Grau, Dídac; Pascual, Marta

    2015-01-01

    La Vall d'Alinyà ha estat tradicionalment una zona agrícola i ramadera, encara que actualment està molt reduïda a causa de l'abandonament dels cultius, la falta del relleu generacional, l'envelliment de la població, la migració a la ciutat, entre d'altres. Aquestes activitats humanes conviuen amb la fauna silvestre de la zona, que en alimentar-se parcialment dels cultius, generalment patates i pèsols, on hi causa diversos impactes. És el cas del porc senglar (Sus scrofa) en especial. Hem obse...

  19. Uso tradicional de vertebrados silvestres en la Sierra Nanchititla, México

    OpenAIRE

    Monroy Vilchis, Octavio; Cabrera García, Leonardo; Suárez, Pedro; Zarco González, Martha Mariela; Rodríguez Soto, Clarita; Urios, Vicente

    2008-01-01

    Los pobladores nativos de México se han caracterizado por poseer conocimiento tradicional sobre las especies de flora y fauna de los ambientes donde habitan, así como por sus prácticas de aprovechamiento. En este estudio se documentó el uso tradicional de vertebrados silvestres por parte de los habitantes de las trece comunidades de mayor influencia de la Reserva Natural Sierra Nanchititla (RNSN), se examinó su disponibilidad como recurso en esta área protegida y se analizó la correlación ent...

  20. USO TRADICIONAL DE VERTEBRADOS SILVESTRES EN LA SIERRA NANCHITITLA, MÉXICO

    OpenAIRE

    Octavio Monroy-Vilchis; Leonardo Cabrera; Pedro Suárez; Martha Mariela Zarco-González; Clarita Rodríguez-Soto; Vicente Urios

    2008-01-01

    Los pobladores nativos de México se han caracterizado por poseer conocimiento tradicional sobre las especies de flora y fauna de los ambientes donde habitan, así como por sus prácticas de aprovechamiento. En este estudio se documentó el uso tradicional de vertebrados silvestres por parte de los habitantes de las trece comunidades de mayor influencia de la Reserva Natural Sierra Nanchititla (RNSN), se examinó su disponibilidad como recurso en esta área protegida y se analizó la correlación ent...

  1. ZOONOSIS TRANSMITIDAS POR ANIMALES SILVESTRES Y SU IMPACTO EN LAS ENFERMEDADES EMERGENTES Y REEMERGENTES

    OpenAIRE

    Santiago Monsalve B; Salim Mattar V.; Marco Gonzalez T

    2009-01-01

    Las enfermedades emergentes y reemergentes originadas desde animales de vida silvestre, pueden ser transmitidas a las poblaciones humanas por contacto directo o por vectores. Las zoonosis determinan una gran problemática social epidemiológica. La relación que tiene las enfermedades emergentes y reemergentes está ligada a la pérdida de la biodiversidad por factores antropogénicos, debido a la destrucción de hábitats naturales, el tráfico de fauna y a la pérdida de diversidad genética. Todos es...

  2. Metodologías para la Colecta de Muestras en Fauna Silvestre in situ

    OpenAIRE

    Santiago Monsalve-Buriticá

    2013-01-01

    El estudio de la fauna silvestre implica el manejo de las poblaciones y su hábitat, ya sea para el aprovechamiento de las especies cinegéticas y de importancia comercial, el control de las poblaciones que causan daño a los intereses humanos, o para la conservación de especies amenazadas. Determinar los diferentes métodos para la colecta de muestras en la investigación del médico de la conservación se hace una actividad transversal a otras ciencias de forma transdisciplinaria cuando se trabaja...

  3. IMPACT OF PARASITISM BY GREGARINES (Nematopsis sp) IN FARMING SHRIMP Litopenaeus vannamei

    OpenAIRE

    Francisco M. Guzmán-Sáenz; Roberto Pérez-Castañeda; Gilberto Gutiérrez-Salazar; Pablo González-Alanís; Mario Hernández-Acosta; Jesús G. Sánchez-Martínez

    2014-01-01

    Infestation of gregarines from genus Nematopsis in Pacific white shrimp Litopenaeus vannamei is commonly associated with a decrease in production and low weight, as well as a possible predisposition to viral infections. The goal of our past research was to evaluate the effect of the Nematopsis sp. gregarine parasitosis on the growth of farm-grown Litopenaeus vannamei shrimp. Four cages were built with PVC pipes and plastic screens with a 6mm light diameter, 1,7m in height, and 1,5m in width ...

  4. Métodos de estudio de la reproducción en hembras de ungulados silvestres

    Directory of Open Access Journals (Sweden)

    Riveros F., José Luis.

    2010-10-01

    Full Text Available ResumenEl presente trabajo tiene por objetivo describir el estado actual yproyecciones de las técnicas de estudio de la reproducción en hembras de ungulados silvestres. Inicialmente se caracteriza su estrategia reproductiva y las limitaciones en el conocimiento de su fisiología, dadas por las dificultades en la aplicación de las técnicas utilizadas en especies domésticas. Posteriormente, se describen las técnicas de estudio más utilizadas en ungulados silvestres, comenzando con estudios etológicos, que permiten caracterizar la estación reproductiva mediante la observación de frecuencias de cortejo, monta y partos.SummaryThe present review is focused on the recent advances and perspectives on female ungulates reproduction research techniques. First the reproductive strategy and the reproduction knowledge limitation given by application difficulties of techniques used in domestic species are characterized. Second the mean methods for studies on reproductive morphology and physiology are described beginning with behavior studies that allow reproductive season characterization by the observation of courtship, mate and birth frequencies.

  5. Arbovírus Ilheus em aves silvestres (Sporophila caerulescens e Molothrus bonariensis

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    Pereira Luiz Eloy

    2001-01-01

    Full Text Available OBJETIVO: Relatar o isolamento do vírus Ilheus no Estado de São Paulo e avaliar o seu impacto para a saúde pública. MÉTODOS: O isolamento de vírus foi realizado em camundongos albinos Swiss, a partir de sangue de aves silvestres, capturadas com redes de espera tipo mist net, armadas no nível do solo, no Parque Ecológico do Tietê, São Paulo. A identificação das cepas isoladas foi feita pelos testes de inibição da hemaglutinação, fixação de complemento e neutralização em camundongos. Amostras de plasma de aves e de mamíferos silvestres foram submetidas à pesquisa sorológica para detecção de anticorpos inibidores de hemaglutinação. RESULTADOS: Foram isoladas duas cepas do vírus Ilheus em sangue de aves das espécies Sporophila caerulescens e Molothrus bonariensis e detectados anticorpos em aves das espécies Columbina talpacoti, Geopelia cuneata, Molothrus bonariensis e Sicalis flaveola, em sagüis das espécies Callithrix jacchus e Callithrix penicillata e no quati Nasua nasua. CONCLUSÕES: O isolamento do vírus Ilheus e a detecção de anticorpos específicos em aves residentes, migratórias e de cativeiro, em sagüis e quatis, comprovam a presença desse agente no Parque Ecológico do Tietê. O comportamento migratório de aves silvestres pode determinar a introdução do vírus em outras regiões. Considerando-se a patogenicidade para o homem e a confirmação da circulação desse agente viral em área urbana, freqüentada para atividade de lazer e de educação, o risco de ocorrência de infecção na população humana não pode ser descartado.

  6. Evaluation of the pharmacological treatment of arterial hypertension associated to heart failure in Camarones town. Evaluación del tratamiento farmacológico de la hipertensión arterial asociada a insuficiencia cardiaca en el poblado de Camarones.

    Directory of Open Access Journals (Sweden)

    Maira Quirós Enríquez.

    muestra de 43 pacientes, cifra que representó el 35,2 % del universo de estudio, en seis Consultorios Médicos de la Familia del área urbana del Policlínico Comunitario de Camarones, Palmira, Cienfuegos, durante el primer semestre de 2004. Resultados: El 51,2 % de los pacientes se incluyeron en la clase II de la clasificación de la New York Heart Asociation y el 55,8 % fueron considerados hipertensos grado II. Los antihipertensivos más utilizados fueron el captopril y la clortalidona y dentro de los fármacos asociados a los antihipertensivos se incluyó el dinitrato de isosorbide, la digoxina y el ácido acetil salicílico. El 87,3 % de los pacientes recibían una dosis correcta y en el 88,9 % se siguió un adecuado intervalo de administración. Se consideró adecuada la prescripción en un 65,1 % de los pacientes del estudio. Conclusiones: Los avances en el tratamiento de estas enfermedades se deben a diversos factores. Aunque el estudio muestra que el tratamiento de los pacientes de la serie es adecuado, debe mejorarse siempre que sea posible.

  7. Mating systems in caridean shrimp (Decapoda: Caridea and their evolutionary consequences for sexual dimorphism and reproductive biology Sistemas de apareamiento en camarones carideos (Decapoda: Caridea y sus consecuencias evolutivas en el dimorfismo sexual y biología reproductiva

    Directory of Open Access Journals (Sweden)

    CRISTIÁN CORREA

    2003-06-01

    species with search & attend the situation is variable, and only some males benefit by acquiring large size while others do better by searching or changing sex. In contrast to other crustaceans free-living shrimp do not exhibit precopulatory mate guarding and they are relatively unaggressive. The characteristic and efficient escape behavior of shrimp using their contractile abdomen might be one important trait that has constrained the evolution of such behaviors. Our review indicates that the mating systems of caridean shrimp depend both on environmental and on biological characteristicsEn este articulo revisamos relaciones funcionales y evolutivas entre los sistemas de apareamiento de camarones carideos, y características específicas tales como biología/ecología general, sistemas sexuales, conducta y morfología. Basados en reportes bibliográficos se describió cuatro sistemas de apareamiento, y un quinto fue reconocido pero la informaci��n disponible fue insuficiente para describirlo en detalle. `Monogamia' ocurre en muchas especies que habitan en refugios monopolizables o huéspedes, especialmente cuando las condiciones ambientales restringen la probabilidad de encuentros entre conespecíficos. En contraste, las especies de vida libre experimentan encuentros más frecuentes y los machos pueden dominar o buscar: En el sistema de apareamiento `dominancia vecinal' los machos más grandes rinden mayor éxito reproductivo porque son los que tienen el mejor desempeño en la competencia agresiva por las hembras receptivas. En el sistema `pura búsqueda' son los machos pequeños los que lo hacen mejor porque su agilidad les permite buscar pareja eficazmente entre la población. El cuarto sistema de apareamiento es `búsqueda & compañía' que ocurre en simbiontes solitarios que experimentan condiciones demográficas y ecológicas variables: dependiendo de las condiciones ambientales y del estado ontogenético, puede ser conveniente para los machos buscar o permanecer

  8. Deep-water shrimp fisheries in Latin America: a review Pesquerías de camarones de aguas profundas en América Latina: una revisión

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    Ingo S Wehrtmann

    2012-09-01

    á expandiendo sus actividades hacia aguas profundas. Las características del ciclo de vida de estos recursos de aguas profundas los hacen más vulnerables a la explotación que la mayoría de los recursos de aguas someras. Además, la falta de información sobre la ecología de la mayoría de las especies de aguas profundas constituye una limitación importante para el desarrollo e implementación de estrategias de manejo. Este escenario ha causado preocupación sobre la sustentabilidad de estos recursos y de los posibles impactos ambientales en los ecosistemas de aguas profundas. La pesca comercial en América Latina se extiende también hacia aguas profundas y, considerando las preocupaciones anteriormente mencionadas, se requiere la necesidad de compilar la información disponible sobre los recursos de camarones de aguas profundas y la situación actual de estas pesquerías en América Latina. Esta revisión se enfoca en México, Centro América, Perú, Chile y Brasil y describe las especies explotadas, la flota pesquera (siempre cuando sea disponible, las estadísticas pesqueras y las estrategias de manejo. Un total de 17 especies (10 spp. de Penaeoidea y 7 spp. de Caridea son de interés comercial en América Latina; sin embargo, camarones de aguas profundas solo se pescan actualmente en Costa Rica, Colombia y Chile. Un plan de manejo implementado existe en Chile y Colombia, mientras que en Brasil se aprobaron regulaciones sobre la pesca de camarones de la familia Aristeidae, pero éstas nunca han sido implementadas. Considerando la falta de información sobre la biología de los camarones de aguas profundas, lo que dificulta el desarrollo de estrategias adecuadas de manejo, se requiere urgentemente mejorar la comunicación y colaboración entre los diferentes actores en Latinoamérica. Se sugiere establecer un banco de datos abierto para búsquedas y actualizarlo constantemente, lo que servirá como fuente valiosa de información para investigadores y quienes deben tomar

  9. Poblaciones silvestres de Tagetes filifolia lag. En el centro-sur de México

    OpenAIRE

    Miguel Ángel Serrato Cruz; Juan Saúl Barajas Pérez

    2006-01-01

    Aquí se presentan los primeros informes sobre aspectos ambientales y la morfológicos de 102 colectas de poblaciones silvestres de Tagetes filifolia Lag. obtenidas en la Región Centro-Sur de México, así como información sobre sus usos. La especie se encontró desde los 750 hasta 2686 m de altitud, en 16 regiones que varían en climas: templado (Cw), semicálido A (C) y (A) C, cálido (A) y semiseco (BS), con 27 subtipos climáticos. Se registraron gradientes de expresión morfológicos de la planta e...

  10. Orientalismos peninsulares en el levante andaluz. Nombres y usos de algunas plantas silvestres

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    Torres Montes, Francisco

    2000-06-01

    Full Text Available The author describes sorne wild plants from the provinces of Almería, Granada and Jaén (Andalusia, Spain that have folk names from Eastern Iberia. For each plant he provides the scientific and folk name, the isoglottic line and the lexical arca. He also informs about the use of these plants in folk and household medecine, religious rites, animal husbandry, etc.En este estudio se presentan algunas plantas silvestres de Almería, Granada y Jaén cuyos nombres vulgares proceden del oriente peninsular. De cada planta se da, junto al nombre vulgar y científico, la isoglosa o límite de los orientalismos y sus correspondientes áreas léxicas. Al mismo tiempo se hace el estudio etnográfico de sus usos en la medicina popular o doméstica, ritos religiosos, ganadería, etc.

  11. Arbovírus Ilheus em aves silvestres (Sporophila caerulescens e Molothrus bonariensis)

    OpenAIRE

    Pereira Luiz Eloy; Suzuki Akemi; Coimbra Terezinha Lisieux Moraes; Souza Renato Pereira de; Chamelet Esther Luiza Bocato

    2001-01-01

    OBJETIVO: Relatar o isolamento do vírus Ilheus no Estado de São Paulo e avaliar o seu impacto para a saúde pública. MÉTODOS: O isolamento de vírus foi realizado em camundongos albinos Swiss, a partir de sangue de aves silvestres, capturadas com redes de espera tipo mist net, armadas no nível do solo, no Parque Ecológico do Tietê, São Paulo. A identificação das cepas isoladas foi feita pelos testes de inibição da hemaglutinação, fixação de complemento e neutralização em camundongos. Amostras d...

  12. Leishmaniose tegumentar alviericana na Ilha Grande, Rio de Janeiro: III. reservatórios silvestres e comensais

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    Nelson A. de Araújo Filho

    1981-12-01

    Full Text Available Em 203 animais silvestres e comensais examinados na Praia Vermelha, Ilha Grande, município de Angra dos fíeis, RJ, durante o estudo de um surto de Leishmaniose Tegumentar Americana, foram encontrados 2 exemplares de Proechimys dimidiatus, com lesões hipocrômicas nas extremidades das orelhas, e 1 exemplar de Rattus norvegicus norvegicus, com úlcera de dorso, cuja histopatologia revelou a presença de Leishmania sp. nos 3 exemplares.

  13. Aislamiento de Vibrio sp. a partir de órganos internos de Dormitator latifrons (Richarson, 1844 silvestres – Reporte preliminar

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    Raúl Mendoza-Rodríguez

    2006-09-01

    Full Text Available Se informa el hallazgo de Vibrio sp.utilizando medio de cultivo selectivoTCBS de muestras de tracto intestinal deejemplares de “monengue” Dormitatorlatifrons silvestres, especie que sealimenta principalmente de detritus enambientes inundados de zonas costeras.This report it shows the finding results ofVibrio sp. using selective culture TCBSfrom intestine samples of fishes “Pacificfat sleepers” Dormitator latifronssavages, species that is fed mainly ondetritus in flooded environments ofcoastal zones.

  14. Evidences of abundant hemocyanin variants in shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Zhao, Xianliang; Guo, Lingling; Lu, Xin; Lu, Hui; Wang, Fan; Zhong, Mingqi; Chen, Jiehui; Zhang, Yueling

    2016-09-01

    Hemocyanin (HMC) is a multifunctional immune molecule present in mollusks and arthropods and functions as an important antigen non-specific immune protein. Our previous evidences demonstrated that Litopenaeus vannamei HMC might display extensive molecular diversities. In this study, bioinformatics analysis showed dozens of variant sequences of the HMC subunit with higher molecular weight from L. vannamei (LvHMC). Three variant fragments, named as LvHMCV1-3, which shared 85-99% nucleotide identity with that of the classical form of LvHMC (AJ250830.1), were cloned and characterized. Spatial expression profiles showed that LvHMCV1-3 had different tissue-specific distribution, which were affected by stimulation with six pathogenic bacteria, including Escherichia coli K12, Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio fluvialis, Streptococcus pyogenes and Staphylococcus aureus, with each variant fragment showing a specific stress pattern to different bacterial pathogens. Full length cDNA of LvHMCV3 was further cloned and characterized. The deduced amino acid sequence shared 92% identity with that of LvHMC, possessed a conserved structure characteristic of the HMC family and could be clustered into one branch along with other arthropod HMC in a phylogenetic tree. In addition, the recombinant protein of LvHMCV3 (rLvHMCV3) showed obvious agglutination activities against three aquaculture pathogenic bacteria including E. coli K12, V. parahaemolyticus and S.aureus at concentrations ranging from 31.25-62.5g/mL. It also showed obvious antibacterial activity against V. parahaemolyticus at concentrations 0.02-0.5mg/mL, and possessed the best inhibitive effects compared with those of rLvHMCV4 and rLvHMC. Co-injection of V. parahaemolyticus and rLvHMCV3 in L. vannamei showed significant decrease of the mortality rate at 24-72h after injection. Therefore, these studies suggested that L. vannamei had abundant HMC variants, which possessed obvious resistance to pathogenic

  15. Epidemiology and control of wild rabies in Italy / Epidemiologia e profilassi della rabbia silvestre in Italia

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    Armando Giovannini

    1991-07-01

    Full Text Available Abstract The authors analyse biological characteristics of the fox Vulpes vulpes with regard to the epidemiology of sylvatic rabies in Italy. Emphasis is put on the lack of deepen studies both on fox population densities, and on important behavioural habits. A retrospective analysis of wild rabies epidemics in Italy from 1977 to 1988 has shown: 1 the spread of rabies by 20 to 80 km/year; 2 the existence of three/four year oscillations, clearly evident in single epidemics, but masked in the total number of cases in Italy; 3 the mosaic-like pattern of the final phase of the epidemic; 4 the extinction of outbreaks following the mosaic phase, in a few cases not correlated with human intervention. The authors criticize the control measures enforced in Italy, on the bases of the inefficacy and inefficiency of the thinning out programme and, on the contrary, the efficiency of the oral vaccination of the fox population. Riassunto Gli autori analizzano la biologia della Volpe (Vulpes vulpes in relazione all'epidemiologia della rabbia silvestre in Italia; sottolineano la grave carenza di dati nel nostro Paese sia sulla densità di popolazione della Volpe, sia su aspetti di ecologia comportamentale importanti per l'epidemiologia della rabbia silvestre in Italia dal 1977 al 1988 rilevando: 1 una velocità di avanzamento variabile da 20 a 80 km/anno; 2 una ciclicità tri-quadriennale per singola epidemia che può essere mascherata se si considera erroneamente la sommatoria dei casi accertati; 3 l'andamento a mosaico delle fasi finali delle epidemie; 4 l'estinzione dei focolai successivamente alla fase a mosaico, in alcuni casi non correlabile all'intervento dell'uomo. Infine, gli autori analizzano in modo critico la profilassi della rabbia nella Volpe attuata in Italia, rilevando la scarsa efficacia ed inefficienza dello sfoltimento e, in contrapposizione, l'efficacia e l'economicità della vaccinazione

  16. Parasitos de aves e mamíferos silvestres em cativeiro no estado de Pernambuco

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    Pauline Marie de Souza Santos

    2015-09-01

    Full Text Available Resumo: Os animais silvestres são hospedeiros de uma grande variedade de parasitos que podem interferir em sua conservação ex situ. O objetivo deste estudo foi identificar os parasitos gastrointestinais (PGI e ectoparasitos dos animais do Centro de Triagem de Animais Silvestres (CETAS do Instituto Brasileiro de Meio Ambiente e Recursos Naturais Renováveis (IBAMA de Recife, Pernambuco, além de determinar os aspectos do manejo em cativeiro que possam estar relacionados com os parasitos identificados. Foram coletados ectoparasitos e amostras fecais de 223 aves e mamíferos, as quais foram processadas pelos métodos: microscopia direta, flutuação e sedimentação. Helmintos e/ou protozoários foram detectados em 91 (40,8% amostras fecais, sendo 64 (70,3% de aves e 27 (29,7% de mamíferos. Ovos de Capillaria sp., Ascaridida, Spirurida e oocistos de Eimeria sp. foram detectados nas amostras fecais das aves, enquanto ovos de Trichuris trichiura, Strongyloides sp., Toxocara canis, Ancylostoma sp., Strongylida e oocistos de Coccídios foram encontrados nas amostras fecais de mamíferos. Os ectoparasitos identificados em aves foram Colpocephalum turbinatum, Kurodaia (Kurodaia fulvofasciata, Halipeurus sp., Naubates sp., Saemundssonia sp., Austromenopon sp., Paragoniocotes sp., Brueelia sp., Myrsidea sp. and Pseudolynchia sp., enquanto em mamíferos os ectoparasitos identificados foram Rhipicephalus sanguineus, Amblyomma varium, A. calcaratum, A. nodosum, Ornithodoros talaje e Ctenocephalides felis felis. A. calcaratum e O. talaje são registrados pela primeira vez em Pernambuco e T. tetradactyla é apresentado como novo hospedeiro de O. talaje. Nenhum dos animais estudados apresentou sinais clínicos em decorrência da infecção/infestação parasitária. Parasitos com potencial zoonótico como T. trichiura, Strongyloides sp., T. canis e Ancylostoma sp. foram identificados em primatas não humanos e carnívoros. Precárias condições estruturais

  17. Isoenzymatic variability in wild potatoes Variabilidade isoenzimática em batata silvestre

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    Beatriz Helena Gomes Rocha

    2001-05-01

    Full Text Available Two species of wild potato Solanum commersonii, subspecies commersonii and malmeanum, and S. chacoense, subspecies muelleri occur in southern Brazil. Their rusticity and easy adaptation to extreme climatic conditions make them valuable for breeding programs. The objective of this work was to analyze the isoenzymatic variability of 113 clones of wild potato subspecies. They were collected and maintained at Embrapa-Centro de Pesquisa Agropecuária de Clima Temperado, at Pelotas, RS, Brazil. Enzymes involved in energetic (group I or in peripherical (group II metabolism constituted the material used. Polyacrylamide horizontal gel electrophoresis was used to analyze peroxidase, aspartate transaminase, phosphoglucomutase and isocitrate dehydrogenase isoenzymes. Solanum spp. has considerable genetic variability for isoenzymatic patterns. Cluster analysis classified the clones into 51 subgroups, based on electrophoretic variants of group I enzymes, and into 89, when group II enzyme variants were added. Genotypic differentiation of S. chacoense muelleri in relation to S. commersonii commersonii and S. commersonii malmeanum is evident when expressed through similarity and cluster analysis.No sul do Brasil ocorrem apenas duas espécies silvestres de batata, Solanum commersonii, com as subespécies commersonii e malmeanum, e S. chacoense, com a subespécie muelleri, de interesse aos programas de melhoramento, pela rusticidade e fácil adaptação a condições climáticas extremas. O objetivo deste trabalho foi analisar a variabilidade isoenzimática de 113 clones de batata silvestre. O material foi coletado e mantido na Embrapa-Centro de Pesquisa Agropecuária de Clima Temperado, em Pelotas, RS. Foram usadas enzimas envolvidas nos metabolismos energético (grupo I e periférico (grupo II. Eletroforese horizontal em gel de poliacrilamida foi empregada para análise de isoenzimas de peroxidase, aspartato transaminase, fosfoglucomutase e isocitrato

  18. Optimization of Process Parameters for Litopenaeus vannamei Seafood Sauce using Response Surface Methodology

    OpenAIRE

    Xinyue Li; Jianfeng Sun; Xiaopeng Cui; Jie Wang

    2014-01-01

    In this study, in order to determine the optimum formulation of Litopenaeus vannamei seafood sauce, Response Surface Methodology (RSM) was employed to investigate the effects of contents of thick broad-bean sauce, sweet soybean paste and Shacha sauce on the sensory quality. Statistical analysis of the results showed that the data were adequately fitted into a second-order polynomial model. The independent parameters of contents of thick broad-bean sauce, sweet soybean paste and the quadratics...

  19. Probiotic P-acidilactici application in shrimp Litopenaeus stylirostris culture subject to vibriosis in New Caledonia

    OpenAIRE

    Castex, Mathieu; Chim, Liet; Pham, Dominique; Lemaire, Pierrette; Wabete, Nelly; Nicolas, Jean-Louis; Schmidely, P; Mariojouls, C.

    2008-01-01

    We studied the effects of a lactic acid bacterium, Pediococcus acidilactici (strain MA 18/5M, CNCM), as a dietary probiotic on growth performance and some nutritional and microbiological aspects of the shrimp Litopenaeus stylirostris. Experimental shrimp culture was carried out over 10 weeks, using floating cages of 14 m(2) each set in earthen ponds, in a New Caledonia farm affected by "Summer syndrome", a septicaemic vibriosis caused by Vibrio nigripulchritudo. The experiment design testing ...

  20. Searching for genetic markers of virulence in Vibrio nigripulchritudo, a Litopenaeus stylirostris shrimp pathogen

    OpenAIRE

    Reynaud, Yann

    2007-01-01

    Since 1997, a new pathology seasonally occurs in new caledonian shrimp farms during the warm season. Diseased Litopenaeus stylirostris shrimp suffer from a systemic vibriosis which was attributed to highly pathogenic Vibrio nigripulchritudo. At the present time, only two farms among 17 are affected by the so called "summer syndrome". In such a context it appears urgent to develop reliable diagnostic tools to detect V. nigripulchritudo strains and to differentiate highly virulent strains from ...

  1. Reproduction of the hawaiian strain of pacific blue shrimp Litopenaeus stylirostris in New Caledonia.

    OpenAIRE

    Pham, Dominique; Patrois, Jacques; Goyard, Emmanuel; Maillez, Jean-rene; Broutoi, Francis; Dufour, Robert; Peignon, Jean-marie; Brun, Pierre; Lambert, Christian; Pita, Etienne

    2007-01-01

    The Pacific blue shrimp Litopenaeus stylirostris was introduced in New Caledonia thirty years ago. Because of its high inbreeding, a SPF strain domesticated in Hawaii, genetically differentiated from the Caledonian strain, had to be imported. The two strains’ reproductive performances were compared at different periods and cross breedings were assessed. The average results show that Caledonian animals give twice as many nauplii than the Hawaiian animals. The best cross breeding is obtained wi...

  2. Effect of dietary protein level on growth and energy utilization by Litopenaeus stylirostris under laboratory conditions

    OpenAIRE

    Gauquelin, F; Cuzon, Gerard; Gaxiola, G.; Rosas, C.; Arena, L.; Bureau, D; Cochard, Jean-claude

    2007-01-01

    A study was conducted using a bioenergetics approach to generate information on energy requirement and feed utilization of Litopenaeus stylirostris. Animals (initial mean weight 21 +/- 1 g were fed ad libitum six experimental diets, ranging from 25 to 58% crude protein (CP), for 50 days. Weight gain increased from 21 to 30 g with increasing dietary protein level. Survival rates averaged 80%. Basal metabolism (HeE) and heat increment of feeding (HiE) were monitored using respirometry. HeE...

  3. Aspectos Epidemiológicos de la Enfermedad de Newcastle en Aves Silvestres de Vida Libre

    Directory of Open Access Journals (Sweden)

    Laura Vargas-Castillo

    2014-12-01

    Full Text Available El virus de la enfermedad de Newcastle (NDV pertenece al género Avulavirus, serotipo paramixovirus aviar tipo 1, de la familia Paramyxoviridae. Es altamente contagiosa ya que se ha demostrado que es capaz de infectar a por lo menos 241 especies, de 27 de los 50 órdenes de aves en el mundo; su patogenicidad está dada por el huésped y el tipo de cepa que presente el animal; se manifiesta con signos clínicos relacionados con el sistema respiratorio, digestivo o nervioso en relación al tipo de cepa. En el humano puede causar conjuntivitis leve y limitada. Esta enfermedad se trasmite por contacto directo con aves enfermas o portadoras, al igual que por contacto con ambientes contaminados. Los estudios relacionados con la presencia del virus de la enfermedad de Newcastle en aves silvestres han sido importantes debido a las implicaciones del virus dentro de la industria avícola ya que genera consecuencias socioeconómicas y sanitarias graves e incide en el comercio internacional de animales.

  4. Relación del Virus del Oeste del Nilo con las Aves Silvestres

    Directory of Open Access Journals (Sweden)

    Diego Soler-Tovar

    2009-06-01

    Full Text Available El Virus del Oeste del Nilo (VON es un Flavivirus que produce una encefalomielitis y puede afectar aves y mamíferos; los efectos varían desde poco notorios hasta la muerte, y en su difusión es importante la participación de especies animales como las aves donde es transmitido a través de mosquitos vectores. El VON se conoce de Uganda desde 1937 y está ampliamente distribuido en África, el oriente y la región sur y tropical de Eurasia. En el Hemisferio Occidental, el VON fue la primera causa de enfermedad en poblaciones de aves y humanos en Nueva York en 1999 y la epizootia ha continuado hasta ahora. La presencia del virus en Norteamérica junto con un número relativamente grande de aves migratorias susceptibles al agente, hacen de este virus de especial atención por su posible impacto sobre la fauna silvestre, los animales domésticos y el hombre en Sudamérica. La infección y mortalidad de aves normalmente precede a las epidemias en humanos y por lo tanto el monitoreo de aves es una herramienta preventiva efectiva que debe ser considerada seriamente.

  5. Development of EST-SSR markers by data mining in three species of shrimp: Litopenaeus vannamei, Litopenaeus stylirostris, and Trachypenaeus birdy.

    Science.gov (United States)

    Pérez, Franklin; Ortiz, Juan; Zhinaula, Mariuxi; Gonzabay, Cesar; Calderón, Jorge; Volckaert, Filip A M J

    2005-01-01

    We report on the data mining of publicly available Litopenaeus vannamei expressed sequence tags (ESTs) to generate simple sequence repeat (SSRs) markers and on their transferability between related Penaeid shrimp species. Repeat motifs were found in 3.8% of the evaluated ESTs at a frequency of one repeat every 7.8 kb of sequence data. A total of 206 primer pairs were designed, and 112 loci were amplified with the highest success in L. vannamei. A high percentage (69%) of EST-SSRs were transferable within the genus Litopenaeus. More than half of the amplified products were polymorphic in a small testing panel of L. vannamei. Evaluation of those primers in a larger testing panel showed that 72% of the markers fit Hardy-Weinberg equilibrium, which shows their utility for population genetic analysis. Additionally, a set of 26 of the EST-SSRs were evaluated for Mendelian segregation. A high percentage of monomorphic markers (46%) proved to be polymorphic by singles-stranded conformational polymorphism analysis. Because of the high number of ESTs available in public databases, a data mining approach similar to the one outlined here might yield high numbers of SSR markers in many animal taxa. PMID:16027992

  6. Diagnóstico da fauna silvestre em empresas florestais brasileiras Diagnosis of wild fauna in brazilian forest companies

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    Giovanna Debortoli Medeiros

    2009-02-01

    Full Text Available Com o objetivo de conhecer a situação atual da fauna silvestre em empresas florestais brasileiras, foram pesquisadas 42 razões sociais, entre Associadas e Co-Participantes da Sociedade de Investigações Florestais (SIF, as quais possuem plantios florestais próprios. As informações foram obtidas com base em questionário enviado às referidas empresas, via correio eletrônico, sendo as respostas obtidas também por esse mecanismo. Entre os vários resultados, destaca-se o fato de que 90,9% das empresas associadas já realizaram levantamentos qualitativos da fauna silvestre. No entanto, de modo geral há notória carência de infra-estrutura nas empresas pesquisadas para atender a trabalhos específicos de manejo e conservação da fauna silvestre.Forty-two companies, associates and co-participants of the Forest Investigation Society (SIF and owners of private forest plantations, were assessed to determine the current situation of wild fauna in Brazilian forest companies. Information was obtained through a questionnaire sent to and received from the companies by e-mail. One of the several results obtained was that 90.9% of the associate companies had already carried out qualitative surveys on wild fauna stands.. On the other hand, in general, there is a total lack of infrastructure in the surveyed companies, to specifically assist in the management and conservation of wild fauna.

  7. Aspectos ecológicos y distribución de Tropaeolum tuberosum ssp. silvestre (Tropaeolaceae en Argentina

    Directory of Open Access Journals (Sweden)

    Eva Bulacio

    2012-06-01

    Full Text Available Tropaeolum tuberosum Ruiz et Pav. ssp. silvestre Sparre es una hierba rizomatosa trepadora que en Argentina fue encontrada en las Sierras de Calilegua (Jujuy y en áreas montanas de Catamarca, aunque de esta última localidad sólo se conoce un único e incompleto ejemplar. Habita quebradas húmedas, entre los 2.100 y 2.650 m s.n.m., en el límite superior del bosque montano. El tallo es grácil de hasta 3 m de largo y la porción subterránea está constituida por rizomas alargados y de hasta 1 cm de diámetro. Estas son las principales diferencias con la ssp. tuberosum, en la cual el tallo es más corto, erguido y desarrolla tubérculos bien definidos.Ecological features and distribution of Tropaeolum tuberosum ssp. silvestre (Tropaeolaceae in Argentina. Tropaeolum tuberosum Ruiz et Pav. ssp. silvestre Sparre is a rhizomatous climbing herb that in Argentina was found in the Sierras of Calilegua (Jujuy and Catamarca mountains, yet of this last locality only is known an one and incomplete specimen. It inhabits humid gorges, between 2100-2650 masl, in the upper limit of the montane forest. The slender stem reaches 3 meters in lenght and the underground portion shows elongated rhizomes up to 1 cm in diameter. These are the main differences with the ssp. tuberosum, in which the stem is shorter, erect and develop well-defned tubers.

  8. Secuencia parcial de un fragmento de ADN de patos silvestres homologo al Complejo Mayor de

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    González-Guzmán Sofía

    2009-04-01

    Full Text Available ResumenLa variación individual de la respuesta inmune esta ligada a ladiversidad funcional y estructural del MHC (por sus siglas en inglesMajor Histocompatibility Complex, TcR (Receptores de células T,inmunoglobulinas (anticuerpos, citoquininas, TNF (por sus siglas eningles Tumor Necrosis Factor, entre otras proteínas. Estas proteínasestán codificadas por múltiples y polimórficos genes, la variabilidad de estos genes constituyen las principales bases, en las variaciones de la resistencia a determinada enfermedad o enfermedades. Estascaracterísticas se han documentado en la gallina doméstica, pero no enel pato. En el presente trabajo se amplifico por PCR un fragmento denucleótidos de ADN genómico de pato doméstico que compartió un100% de similitud con un fragmento del MHC clase II de pollo, quecorrespondió a una secuencia similar a DAB1 (Disabled 1.La secuencia DAB1 también fue amplificada en nueve especies de patos silvestres del género Anas que posterior a un análisis por el polimorfismo del largo de los fragmentos de restricción (PLFR con las enzimas RsaI, BsrI y HindIII, se observó que la especie Anas americana,Anas platyrhynchos diazi y Anas platyrhynchos domesticus fueronsutilmente diferentes a la especie Anas discors, Anas crecca, Anascyanoptera, Anas acuta, Anas streptera y Anas clypeata. Hasta dondesabemos, este es el primer reporte que indica la presencia desecuencias similares a DAB1 en patos silvestres. Este estudio podríaayudar a entender porque los patos podrían ser menos susceptibles apresentar neoplasias que en las gallinas.SummaryThe individual response of the immune system is linked to the functional and structural diversity of the MHC (Major Histocompatibility Complex, TcR (T cell receptor, inmunoglobulines (antibodies, citokines, TNF (Tumor Necrosis Factor, among other proteins. These proteins are coded for multiple and polymorphic genes, the variability of these genes constitutes the main bases, in the

  9. Augusto Boal en la educación social: del teatro del oprimido al psicodrama silvestre

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    Manuel F. Vieites García

    2015-02-01

    Full Text Available La obra de Augusto Boal Teatro del Oprimido se suele situar entre las aportaciones más transcendentales del teatro del siglo XX, en tanto sus dictados teóricos y sus propuestas metodológicas y prácticas han calado en ámbitos de la educación social y teatral relacionados con el desarrollo de la democracia cultural. Pasados cuarenta años desde sus primeras experiencias, en este trabajo se propone una genealogía del teatro del oprimido a partir de una lectura crítica de sus textos fundacionales y de alguna experiencia considerada paradigmática en su desarrollo, para mostrar su naturaleza, aportes, fortalezas y debilidades. Igualmente se muestra el tránsito entre una dimensión más educativa y teatral y otra más centrada en la terapia y el desarrollo personal, para concluir que las técnicas y recursos empleados en teatro del oprimido, que proceden de fuentes muy diversas, siguen teniendo potencialidades indudables en propuestas de intervención social, cultural y educativa propias de la pedagogía teatral y la pedagogía social.Cómo referenciar este artículoVieites García, M. F. (2015. Augusto Boal en la educación social: del teatro del oprimido al psicodrama silvestre. Foro de Educación, 13(18, pp. 161-179. doi: http://dx.doi.org/10.14516/fde.2015.013.018.009 

  10. Caracterización morfológica de accesiones silvestres de guayaba

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    Muñoz florez Jaime Eduardo

    2009-06-01

    Full Text Available Se realizó la caracterización morfológica de 22 accesiones silvestres de guayaba Psidium guajava L. 14 colectadas en el municipio de Restrepo (Valle del Cauca, seis en Armenia (Quindío y dos en Pereira (Risaralda. Se utilizaron 12 descriptores cuantitativos y 10 cualitativos de tallo, hojas y frutos; el análisis de agrupamiento se hizo mediante el coeficiente de Dice-Nei-Li y el promedio aritmético no ponderado (UPGMA. La mayor variabilidad se halló en los descriptores peso de la pulpa (CV = 55.92%, peso (CV = 45.23%, y acidez del fruto (CV = 44.75%. El análisis de agrupamientos con base en caracteres cuantitativos permitió establecer cuatro grupos: las accesiones del grupo A (Armenia, presentaron valores promedio de los descriptores de la calidad del fruto (grados Brix, acidez del fruto y relación grados Brix/acidez y valores altos de contenido de pulpa. La mayoría de accesiones del grupo C (Restrepo tuvieron altos valores de calidad del fruto y bajo contenido de pulpa. El grupo B, constituido por accesiones de Armenia y Pereira, se diferenció por valores bajos en los descriptores de rendimiento del fruto (peso del fruto, de la pulpa y diámetro de la cavidad seminal. Las accesiones del grupo D (Restrepo mostraron valores promedios en calidad y rendimiento del fruto. Los descriptores cuantitativos se reunieron en tres variables sintéticas para rendimiento y calidad del fruto que representaron 76.86% de la variabilidad total.

  11. Caracterización morfológica de accesiones silvestres de guayaba

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    Liliana Jiménez Lozano

    2009-04-01

    Full Text Available Se realizó la caracterización morfológica de 22 accesiones silvestres de guayaba Psidium guajava L. 14 colectadas en el municipio de Restrepo (Valle del Cauca, seis en Armenia (Quindío y dos en Pereira (Risaralda. Se utilizaron 12 descriptores cuantitativos y 10 cualitativos de tallo, hojas y frutos; el análisis de agrupamiento se hizo mediante el coeficiente de Dice-Nei-Li y el promedio aritmético no ponderado (UPGMA. La mayor variabilidad se halló en los descriptores peso de la pulpa (CV = 55.92%, peso (CV = 45.23%, y acidez del fruto (CV = 44.75%. El análisis de agrupamientos con base en caracteres cuantitativos permitió establecer cuatro grupos: las accesiones del grupo A (Armenia, presentaron valores promedio de los descriptores de la calidad del fruto (grados Brix, acidez del fruto y relación grados Brix/acidez y valores altos de contenido de pulpa. La mayoría de accesiones del grupo C (Restrepo tuvieron altos valores de calidad del fruto y bajo contenido de pulpa. El grupo B, constituido por accesiones de Armenia y Pereira, se diferenció por valores bajos en los descriptores de rendimiento del fruto (peso del fruto, de la pulpa y diámetro de la cavidad seminal. Las accesiones del grupo D (Restrepo mostraron valores promedios en calidad y rendimiento del fruto. Los descriptores cuantitativos se reunieron en tres variables sintéticas para rendimiento y calidad del fruto que representaron 76.86% de la variabilidad total.

  12. Hacia el Entendimiento de la Analgesia y Anestesia en Animales Silvestres

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    Farnando Nassar-Montoya

    2010-02-01

    Full Text Available La práctica en especies silvestres que lógicamente conlleva la utilización de fármacos analgésicos y anestésicos, presenta una idiosincrasia propia que tiene que ser entendida desde la conceptualización del significado de la medicina veterinaria. Por esto, estos textos inician con una reflexión a partir del siguiente texto de una sentencia del Tribunal Nacional de Ética Profesional de Medicina Veterinaria y Zootecnia de Colombia: “… La investigada se excusó en que realizó el examen clínico general previa la cirugía, sin embargo, el examen superficial o externo del animal no es suficiente para hacer una evaluación completa del estado de salud como lo exige la norma. Cuando un animal va a ser intervenido quirúrgicamente y sometido a anestésicos que le pueden causar la muerte, se deben reducir los riesgos posibles por lo que, como mínimo se debe evaluar el estado de la función hepática y renal de los animales. La médica veterinaria no realizó exámenes pre-quirúrgicos que descartaran problemas de función hepática o renal, causándole con esta omisión una violación al deber profesional del artículo…”

  13. Interrelaciones entre hospedadores, vectores y parásitos sanguíneos en poblaciones de aves silvestres

    OpenAIRE

    Martínez de la Puente, Josué

    2009-01-01

    El parasitismo es una de las relaciones simbióticas más importantes en la naturaleza. Las aves, como la mayoría de los organismos silvestres, se encuentran con frecuencia afectadas por una enorme diversidad de parásitos, tanto endoparásitos como ectoparásitos. El estudio del parasitismo bajo un prisma ecológico-evolutivo es una de las principales vías de investigación mundial en el campo de la biología evolutiva por la importancia de estos organismos parásitos en la evolución de sus hospedado...

  14. CARACTERIZAÇÃO DAS APREENSÕES DE FAUNA SILVESTRE NO ESTADO DO AMAPÁ, AMAZÔNIA ORIENTAL, BRASIL

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    Miguel Benedito Ferreira Dias Junior

    2014-04-01

    Full Text Available Este artigo objetivou caracterizar as apreensões de fauna silvestre no estado do Amapá. Foram analisados registros constantes dos Autos de Infração Ambiental lavrados pelos órgãos de fiscalização ambiental (Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis, Secretaria de Estado do Meio Ambiente, Instituto de Meio Ambiente e Ordenamento Territorial do Amapá e Batalhão de Polícia Militar Ambiental no período de 2005 a 2009. Os resultados mostraram que foram apreendidos 1.986 animais silvestres que geraram R$ 694.937,00 em multas. As apreensões ocorreram em 13 dos 16 municípios amapaenses, com destaque para Macapá e Santana que concentraram 87% das autuações. As infrações mais cometidas foram a posse ou manutenção de animais em cativeiro e o transporte de carne e de animais vivos com 38,14% e 30,77%, respectivamente. Répteis e aves foram os mais apreendidos com 48% e 45%, enquanto os mamíferos foram os menos apreendidos com 7% das apreensões. Do total de espécimes apreendidos, 736 pertencem a nove espécies constantes da lista de espécies ameaçadas, das quais, sete constam simultaneamente nas listas da IUCN e da CITES. Concluiu-se que a fauna silvestre tem sido utilizada de forma indiscriminada para fins de criação e consumo alimentar nas áreas rurais e urbanas do Estado e, que deficiências na execução das atividades de fiscalização dificultam a coibição dessa atividade ilícita. Palavras-chave: Animais silvestres, espécies ameaçadas, fiscalização, órgãos ambientais. DOI: http://dx.doi.org/10.18561/2179-5746/biotaamazonia.v4n1p65-73

  15. Aspectos ecológicos y distribución de Tropaeolum tuberosum ssp. silvestre (Tropaeolaceae) en Argentina

    OpenAIRE

    Eva Bulacio; Hugo Ayarde

    2012-01-01

    Tropaeolum tuberosum Ruiz et Pav. ssp. silvestre Sparre es una hierba rizomatosa trepadora que en Argentina fue encontrada en las Sierras de Calilegua (Jujuy) y en áreas montanas de Catamarca, aunque de esta última localidad sólo se conoce un único e incompleto ejemplar. Habita quebradas húmedas, entre los 2.100 y 2.650 m s.n.m., en el límite superior del bosque montano. El tallo es grácil de hasta 3 m de largo y la porción subterránea está constituida por rizomas alargados y de hasta 1 cm de...

  16. Contribución a la patología de los carnívoros silvestres.

    OpenAIRE

    Sobrino Menchero, Raquel

    2008-01-01

    España cuenta con una rica comunidad de carnívoros silvestres terrestres. Esta tesis parte de las siguientes hipótesis: (1) los carnívoros terrestres, por su ubicación en la cúspide de la pirámide trófica, pueden ser buenos indicadores de la presencia y frecuencia de enfermedades que afectan a distintos taxones animales. (2) la diversidad paisajística de la Península Ibérica, que se asocia con diferentes comunidades de carnívoros terrestres, podría determinar diferencias en la distribución y ...

  17. MAMÍFEROS INVASORES EN LA PATAGONIA ARGENTINA: EL CONEJO SILVESTRE EUROPEO COMO CASO EMBLEMÁTICO

    OpenAIRE

    Never Antonio Bonino Vassallo

    2010-01-01

    Se brinda una reseña de los estudios biológicos y ecológicos realizados sobre el conejo silvestre europeo (Oryctolagus cuniculus), tal vez el más emblemático de los mamíferos exóticos introducidos en Argentina. En el sector continental argentino el conejo se encuentra establecido, y en proceso de dispersión, principalmente en las provincias de Mendoza y Neuquén (NO de la Patagonia). En esta región, el conejo ha demostrado tener un efecto negativo sobre la economía regional, especialmente e...

  18. Potencial nutracêutico de cogumelos silvestres comestíveis do género Agaricus.

    OpenAIRE

    Pereira, Eliana; Barros, Lillian; Martins, Anabela; Isabel C. F. R. Ferreira

    2011-01-01

    Os cogumelos têm uma enorme importância industrial, nomeadamente nos sectores da indústria alimentar e farmacêutica. O volume comercial relacionado com cogumelos silvestres chegou mesmo a atingir, em 2004, 1,5 mil milhões de euros [1]. Assim, o estudo das suas características químicas e biológicas adquire enorme relevância uma vez que eles constituem bioreactores naturais para a produção de compostos com interesse farmacológico [2,3]. Neste trabalho, apresentaremos um estudo comparativo d...

  19. Infecção natural de roedores silvestres pelo Schistosoma mansoni Natural infection of sylvatic rodents by Schistosoma mansoni

    OpenAIRE

    Tânia Maria Correa Silva; Zilton A. Andrade

    1989-01-01

    No município baiano de Planalto, 47% dos roedores silvestres capturados (Nectomys) estavam infectados pelo Schistosoma mansoni, enquanto a prevalência desta infecção na população humana da área era de 3,26%. Os roedores habitam zonas peridomiciliares, têm hábitos aquáticos e eliminam ovos viáveis do S. mansoni. Albergam número variável de vermes e formam granulomas periovulares pequenos, principalmente no fígado e intestinos, sem fibrose hepática importante ou sinais de hipertensão porta. A d...

  20. Ixodídeos em animais silvestres na Região do Planalto Serrano, Estado de Santa Catarina, Brasil

    OpenAIRE

    Marcia Sangaletti Lavina; Antonio Pereira de Souza; Amélia Aparecida Sartor; Anderson Barbosa de Moura

    2015-01-01

    O conhecimento da fauna de ixodídeos, bem como a potencialidade como transmissores de patógenos aos seus hospedeiros e em alguns casos aos humanos, é de grande importância para o estabelecimento de programas de saúde pública e vigilância epidemiológica. Com o objetivo de identificar ixodídeos de mamíferos silvestres no Planalto Serrano de Santa Catarina foram examinados carrapatos, coletados ou recebidos no Laboratório de Parasitologia e Doenças Parasitárias do Centro de Ciências Agroveteriná...

  1. Opciones de gestión para reducir la cacería ilegal: el caso de la Reserva de Vida Silvestre Amazónica Manuripi, Bolivia

    OpenAIRE

    Espinoza, Sophía Kantuta; Dresdner Cid, Jorge; Chávez Rebolledo, Carlos

    2013-01-01

    La recolección de castaña (Bertholletia excelsa) es la principal actividad forestal no maderable de Bolivia y la fuente de ingresos más relevante para comunidades y propietarios privados que habitan dentro el área Reserva Nacional de Vida Silvestre Amazónica Manuripi. Sin embargo, esta actividad está vinculada a problemas de conservación de la fauna silvestre como consecuencia de la cacería realizada por los recolectores que se internan en el bosque para recoger los frutos de la castaña. La c...

  2. Combined effect of external ammonia and molt stage on the blue shrimp Litopenaeus stylirostris physiological response

    OpenAIRE

    Mugnier, Chantal; Justou, Carole

    2004-01-01

    The effect of ambient ammonia and molt stage on the physiological response of the blue shrimp Litopenaeus stylirostris was studied. Shrimps were submitted to 54.6 mg l(-1) ammonia-N (1.76 mg l(-1) NH3-N) for 24 h. Only shrimps in stages C, D-0, D-1 and D-2 were used for the analysis. Haemolymph was assayed for osmoregulatory capacity (OC), magnesium ion (Mg ion), total proteins, oxyhaemocyanin, lactate and glucose. Molt stage had an effect on OC, Mg ion and total proteins in control shrimps, ...

  3. Influence of probiotics on the growth and digestive enzyme activity of white Pacific shrimp ( Litopenaeus vannamei)

    Science.gov (United States)

    Gómez, R. Geovanny D.; Shen, M. A.

    2008-05-01

    The influence of Bacillus probiotics on the digestive enzyme activity and the growth of Litopenaeus vannamei were determined in this study. The shrimp was treated with five percentages (1.5, 3.0, 4.5, 6.0 and 7.5) of probiotics ( Bacillus spp.) supplemented to the feed and cultured for 45d. The growth measured as the weight gain at the end of culturing was significantly ( Pprobiotic-treated shrimps than that of the control (without receiving probiotics). Activities of protease and amylase, two digestive enzymes of the midgut gland and the intestine were significantly ( Pprobiotic-treated shrimp than in the control.

  4. DIAGNÓSTICO JURÍDICO SOBRE EL DECOMISO Y MANEJO POSTDECOMISO DE FAUNA SILVESTRE EN EL DEPARTAMENTO DEL CHOCÓ

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    Lisneider Hinestroza Cuesta

    2011-12-01

    Full Text Available En este artículo se presenta un estudio de carácter descriptivo sobre los decomisos y el manejo postdecomiso de fauna silvestre en el departamento del Chocó utilizado como corredor para el tráfico y comercialización ilegal; se analizan, a partir del marco jurídico colombiano, la definición de fauna silvestre, decomiso y sus consecuencias jurídicas. Entre el 2005 y marzo del 2011 se realizaron en el Chocó 904 decomisos: 316 aves, 321 reptiles y 264 de mamíferos, 1 anfibio, 1 arácnido y 1 pez. La tendencia ha sido el aumento. La principal opción de disposición de fauna decomisada es la Liberación. Se han impuesto solamente sanciones de tipo administrativo sin reporte en el Registro Único de Infractores Ambientales y no se ha adelantado ninguna investigación de tipo penal

  5. Dietas practicas para el cultivo de Litopenaeus schmitti: una revisión (Practical diets for Litopenaeus schmitti shrimp culture: a review

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    Jaime-Ceballos, Barbarito:

    2006-12-01

    Full Text Available Se resumen los resultados del trabajo desarrollado por investigadorescubanos en el campo de la alimentación y nutrición del camarónblanco Litopenaeus schmitti, referidos a la formulación de alimentosbalanceados para las fases de precría y engorde. Aporta información sobre la respuesta de dicha especie a la inclusión de materias primasconvencionales o no en dietas prácticas, constituyendo una vía deabaratamiento de las mismas mediante el uso de ingredientes nacionales. Además se muestran los resultados obtenidos en cuanto aempleo de aglutinantes, tamaños de partículas e investigaciones básicas aplicadas a esta actividad. Estos estudios han servido de base para la obtención de alimentos comerciales eficientes que sustentan el desarrollo de la camaronicultura en Cuba, propiciando el diseño de alimentos acordes a las necesidades y posibilidades del país, constituye un valioso material para el conocimiento y desarrollo del cultivo en cuanto a Results of the research workdeveloped by Cuban investigators on feeding and nutrition of white shrimp Litopenaeus schmitti, referred the food formulation for nursery and grow-out phases. It contributes information on the answer of this species to the inclusion of conventional raw materials or not in practical diets, constituting a via to reduce prices trough the use of national ingredients. In addition results obtained as far as use of aglutinantes, sizes of particles and basic investigations applied to this activity are shown. These studies have served as base to obtain efficient commercial diets that have supported the development of shrimp culture in Cuba, facilitating the designof artifitial food according to the necessities and possibilities of the country, constituting a valuable material for the knowledge anddevelopment of the culture as far as feeding and nutrition.H

  6. Replacement of fish meal with black soldier fly meal in practical diets for Pacific whiteleg shrimp Litopenaeus vannamei

    Science.gov (United States)

    Shrimp account for 15 percent of the total value of internationally traded fishery products, and currently are the largest single aquaculture commodity in value terms. The Pacific whiteleg shrimp (Litopenaeus vannamei) generated USD 11 billion from a production volume of 2.7 million metric tons (mm...

  7. Comparison of immune response of Pacific white shrimp, Litopenaeus vannamei, after multiple and single infections with WSSV and Vibrio anguillarum

    Science.gov (United States)

    Our previous study demonstrated that Pacific white shrimp (Litopenaeus vannamei) infected by multiple pathogens showed higher mortality and death occurred more quickly than those infected by a single pathogen [1]. For better understanding the defense mechanism against white spot syndrome virus (WSSV...

  8. Sensitivity of Larvae and Adult and the Immunologic Characteristics of Litopenaeus vannamei under the Acute Hypoxia

    Directory of Open Access Journals (Sweden)

    Hailong Zhou

    2014-01-01

    Full Text Available Litopenaeus vannamei is one of the most commercially important species of shrimp in the world. In this study, we performed acute hypoxia tests with Litopenaeus vannamei to estimate 12 h median lethal concentration (LC50 values at different life stages. The results indicated that the 12 h LC50 values were significantly different in different life stages of shrimp (P<0.05. The maximum value of 12 h LC50 was 2.113 mg L−1 for mysis III, and the minimum value was 0.535 mg L−1 for adult shrimp with an average total length of 6 cm. The study also determined the hemocyanin concentration (HC and the total hemocyte counts (THC in the conditions of hypoxia and reoxygenation. These results showed that the THC decreased and the HC increased under hypoxia, and the THC increased and the HC decreased in the condition of reoxygenation. These results can provide fundamental information for shrimp farming and seedling and also can guide the breeding selection, as well as being very helpful to better understand the hypoxia stress mechanism of shrimp.

  9. Culturable fungal diversity of shrimp Litopenaeus vannamei boone from breeding farms in Brazil

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    Lidiane Roberta Cruz da Silva

    2011-03-01

    Full Text Available Litopenaeus vannamei, which is the most common shrimp species cultivated in the northeast of Brazil, is very susceptible to microbial diseases, and this consequently affects productivity. There are reports of bacteria, viruses and protozoa in these shrimp, but not fungi. This study aims to isolate and identify fungi present in shrimp Litopenaeus vannamei, and in their nursery waters, at two breeding farms in Brazil. The pathogenic potential of the isolates was assessed through the qualitative detection of proteases and aflatoxin B production. The 146 isolated fungi comprised 46 species. Aspergillus, Penicillium and Furarium were the three most relevant genera and Aspergillus flavus was the predominant species with a total of 33 isolates. Most of the isolated species are known as potentially pathogenic to humans and other animals. Eighteen isolates of A. flavus and two of A. parasiticus were able to produce aflatoxin B and 33 out of the 46 species produced protease, indicating that these fungi may also become pathogenic to shrimp and their consumers.

  10. Neonatal cannibalism in cage-bred wild rabbits (Oryctolagus cuniculus Canibalismo neonatal en conejos silvestres (Oryctolagus cuniculus alojados en jaula

    Directory of Open Access Journals (Sweden)

    P González-Redondo

    2008-01-01

    Full Text Available The aim of this study was to analyse the occurrence of neonatal cannibalism by individually outdoors cage-bred wild rabbit does (Oryctolagus cuniculus. Ninety eight parturitions that were gathered from 19 female cage-born wild rabbits during five consecutive years were monitored for cannibalism incidence. This alteration in maternal behaviour, which was exclusively limited to the peripartum period, had a high incidence (13.3% of parturitions and was significantly associated with inadequate maternal behaviour such as not using straw or hair in nestbuilding or giving birth outside the nest box. In 84.6% of the parturitions with occurrence of cannibalism the does did not introduce hair into the nest boxes, and in 92.3% of the parturitions with cannibalised kits the does did not introduce straw into the nest boxes. Cannibalism was also associated with a large proportion of rabbits that gave birth outside the nest boxes (53.8%. It is discussed that cannibalism practiced by wild rabbit does in captivity is a manifestation of the failure of maternal behaviour, a consequence of the stress they experience in captivity.El objetivo de este estudio fue analizar la práctica de canibalismo neonatal por conejas silvestres (Oryctolagus cuniculus alojadas individualmente en jaulas ubicadas al aire libre. Se controló la incidencia de canibalismo en 98 partos producidos durante cinco años consecutivos por 19 conejas silvestres nacidas en jaula. Esta alteración de la conducta maternal, que estuvo limitada exclusivamente al periparto, tuvo una elevada incidencia (13,3% de los partos y estuvo asociada significativamente con una inadecuada conducta maternal, tal como la no introducción de pelo y paja en los nidales por parte de las conejas o los partos fuera del nidal. En el 84,6% de los partos en los que ocurrió canibalismo las conejas no introdujeron pelo en los nidales, y en el 92,3% de los partos con gazapos canibalizados las conejas no introdujeron paja en

  11. Atividade e moléculas antioxidantes de cogumelos silvestres comestíveis do género Agaricus

    OpenAIRE

    Martins, Anabela; Pereira, Eliana; Barros, Lillian; Isabel C. F. R. Ferreira

    2012-01-01

    Os cogumelos contêm vários compostos antioxidantes nomeadamente, compostos fenólicos (e.g. ácidos fenólicos), tocoferóis e carotenóides. Entre as substâncias biologicamente ativas presentes nos cogumelos, os compostos fenólicos têm atraído muita atenção devido às suas excelentes propriedades como antioxidantes e ao seu potencial anti-inflamatório ou antitumoral [1,2]. Neste trabalho, foi avaliado o potencial antioxidante de seis espécies silvestres comestíveis do género Agaricus: Agaricus bis...

  12. El Virus del Nilo Occidental y las Aves Silvestres de Colombia: experiencia en San Andrés Islas

    Directory of Open Access Journals (Sweden)

    Diego Soler-Tovar

    2006-06-01

    Full Text Available El Virus del Nilo Occidental (VNO es una zoonosis transmitida por zancudos (Culex spp., por lo tanto pertenece al grupo de los Arbovirus, se clasifica dentro del género Flavivirus, familia Flaviviridae. El virión es de cadena sencilla, ARN de secuencia positiva de aproximadamente 11 kb (kilobases. Pertenece al serocomplejo de la Encefalitis Japonesa. Debido a la actualidad e importancia del VNO, entre septiembre de 2005 y febrero de 2006, se realizo una investigación entre la Línea de Microbiología y Epidemiología Veterinaria de la Facultad de Medicina Veterinaria y de Zootecnia de la Universidad Nacional de Colombia y la Fundación ProAves, con el apoyo del Centro de Control y Prevención de Enfermedades de los Estados Unidos (CDC, para detectar el VNO en aves silvestres de San Andrés Islas, Colombia.

  13. Are camouflaged seeds less attacked by wild birds? Sementes camufladas são menos atacadas por aves silvestres?

    Directory of Open Access Journals (Sweden)

    Alexandre de Almeida

    2010-04-01

    Full Text Available Wheat, corn and rice crops in Brazil use seeds treated with systemic insecticide/nematicide carbofuran, mixed to rhodamine B red dye. Carbofuran is toxic and rhodamine B is attractive to wild birds that eat up these seeds, resulting in notable mortality during planting. A field experiment was performed in southeast Brazil to evaluate if camouflaged seeds would be less consumed by wild birds in comparison to commercial seeds with red-colored rhodamine B and aposematic blue seeds. Camouflaged seeds were less removed than seeds with rhodamine B and natural colors. The camouflaging was more effective in the presence of irregularities and litter. There was no removal of blue-colored seeds. As legislation requires treated seeds to receive a different color to avoid accidents with humans, camouflaging may be used as replacement of rhodamine B to reduce mortality rates of wild birds.Plantações de trigo milho e arroz no Brasil utilizam sementes tratadas com o inseticida e nematicida sistêmico carbofuran, associado ao corante vermelho rodamina B. O carbofuran é tóxico e a rodamina B é atrativa às aves silvestres, as quais consomem estas sementes, resultando em notável mortalidade durante o plantio. Um experimento realizado em campo agrícola no sudeste do Brasil mostrou que sementes camufladas foram menos removidas por aves silvestres do que sementes com rodamina B. A camuflagem foi potencializada em presença de irregularidades e serrapilheira no solo. Não houve remoção de sementes de cor azul, mas a formulação granular de cor azul tem causado mortalidade de aves nos USA e Canadá. Como sementes tratadas com agrotóxicos devem, de acordo com a legislação, receber coloração diferenciada para evitar acidentes com humanos, a camuflagem pode ser utilizada, substituindo a rodamina B.

  14. Aplicaciones del Triage en la Medicina de Fauna Silvestre, Exótica y de Zoológico

    Directory of Open Access Journals (Sweden)

    Nestor Varela

    2012-06-01

    Full Text Available En este artículo el autor revisa los elementos conceptuales y metodológicos del triage como herramienta en la atención médica de animales silvestres y exóticos, con objeto de compendiar los aspectos esenciales para que el clínico veterinario realice la evaluación y categorización inicial de los pacientes que ingresan por emergencia a las clínicas, zoológicos, centros de rehabilitación, o que son atendidos en el campo durante accidentes naturales, así como la metodología para establecer las necesidades de abordamiento clínico, condiciones de riesgo y lugar apropiado de tratamiento de estos pacientes. Se incluyen en el documento aspectos básicos sobre normas de conducta ética y legislación, con énfasis en Colombia; además de algunas generalidades sobre manejo de fauna silvestre. Abstract. In this article the author revises conceptual and methodological elements of triage into the wildlife and exotic animals medical care, with object of summarizing essential aspects so that the clinical veterinarian carries out the evaluation and the patients' initial categorization that enter for emergency to the clinics, zoos, rehabilitation centers and that they are assisted in the field, as well as the methodology to establish the necessities of clinical approach, conditions of risk and appropriate place of these patients' treatment. They are included in the document basic aspects about norms of ethical behavior, legislation and some generalities on wildlife management, with emphasis in Colombian legislation.

  15. Ixodídeos em animais silvestres na Região do Planalto Serrano, Estado de Santa Catarina, Brasil

    Directory of Open Access Journals (Sweden)

    Marcia Sangaletti Lavina

    2015-10-01

    Full Text Available O conhecimento da fauna de ixodídeos, bem como a potencialidade como transmissores de patógenos aos seus hospedeiros e em alguns casos aos humanos, é de grande importância para o estabelecimento de programas de saúde pública e vigilância epidemiológica. Com o objetivo de identificar ixodídeos de mamíferos silvestres no Planalto Serrano de Santa Catarina foram examinados carrapatos, coletados ou recebidos no Laboratório de Parasitologia e Doenças Parasitárias do Centro de Ciências Agroveterinárias – CAV/UDESC no período 2001 a 2011. Os ixodídeos eram provenientes de animais silvestres que passaram pelo processo de triagem do Hospital de Clínicas Veterinárias (HCV do CAV e de animais encontrados mortos em rodovias do Estado. De acordo com as chaves dicotômicas específicas identificou-se Amblyomma aureolatum coletados em Pseudalopex gymnocercus, Lycalopex gymnocercus, Leopardus pardalis, Cerdocyon thous, Leopardus tigrinus, Puma concolor e Bufo sp.; A. dubitatum em Hydrochoerus hydrochoeris; A. rotundatum em Puma concolor e Bohtrops sp.; A. ovale em Bufo sp.; e Rhipicephalus microplus em Mazama gouazoubira. Foi identificado um exemplar de A. longirostre que se encontrava em uma residência da área urbana do município de Lages. As espécies A. rotundatum em Bohtrops sp. e em Puma concolor; e A. longirostre, encontrada no ambiente, foram relatadas pela primeira vez no estado de Santa Catarina, Brasil. 

  16. Antimutagenicity and Antiproliferative Studies of Lipidic Extracts from White Shrimp (Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Carolina Moreno-Félix

    2010-11-01

    Full Text Available An organic extract from fresh shrimp (Litopenaeus vannamei was studied for antimutagenic and antiproliferative properties using Salmonella typhimurium tester strains TA98 and TA100 with metabolic activation (S9 and a cancer cell line (B-cell lymphoma, respectively. Shrimp extract was sequentially fractionated by thin layer chromatography (TLC and each fraction was tested for antimutagenic and antiproliferative activities. Crude organic extracts obtained from shrimp reduced the number of revertants caused by aflatoxina B1, showing a dose-response type of relationship. Sequential TLC fractionation of the active extracts produced several antimutagenic and/or antiproliferative fractions. These results suggested that the lipid fraction of the tested species contained compounds with chemoprotective properties that reduce the mutagenicity of AFB1 and proliferation of a cancer cell line.

  17. Metal content of the gulf of California blue shrimp Litopenaeus stylirostris (Stimpson).

    Science.gov (United States)

    Frías-Espericueta, M G; Izaguirre-Fierro, G; Valenzuela-Quiñonez, F; Osuna-López, J I; Voltolina, D; López-López, G; Muy-Rangel, M D; Rubio-Castro, W

    2007-08-01

    The blue shrimp Litopenaeus stylirostris is the main target species of the Gulf of California shrimping fleet, and its heavy metal content might be of concern for human health because of the increasing contamination of the Gulf. The Cd content of shrimp caught by commercial trawlers ranged from 0.38 to 1.05 microg/g and the mean value was significantly lower in the northern fishing grounds. Pb ranged from 3.19 to 9.59 microg/g and was significantly higher in the northern area. There were no significant geographic difference in the case of Cu and Zn (respective means = 25.4 and 57.8 microg/g). The mean values of all metals show that none is of particular concern for human health. PMID:17639322

  18. Essai de substitution de l'érythromycine en élevage larvaire de Litopenaeus stylirostris

    OpenAIRE

    Herlin, Jose

    2001-01-01

    Cette fiche biotechnique présente deux expérimentations visant à réduire voire substituer l'usage de l'érythromycine en élevage larvaire de Litopenaeus stylirostris. La première testant la bactérie Roseobacter gallaeciensis comme probiotique, associée ou non à des changements d'eau différents, n'a pas donné de résultats positifs malgré des résultats intéressants de croissance et de survie, obtenus sur des larves de mollusques au Laboratoire de Physiologie des Invertébrés de L'IFREMER de Brest...

  19. Involvement of penaeidins in defense reactions of the shrimp Litopenaeus stylirostris to a pathogenic vibrio.

    Science.gov (United States)

    Munoz, M; Vandenbulcke, F; Garnier, J; Gueguen, Y; Bulet, P; Saulnier, D; Bachère, E

    2004-04-01

    The present study reports for the first time the involvement of an antimicrobial peptide in the defense reactions of a shrimp infected by a pathogenic Vibrio, Vibrio penaeicida. New members of the penaeidin family were characterized in the shrimp Litopenaeus stylirostris by RT-PCR and RACE-PCR from hemocyte total RNAs, and by mass spectrometry detection and immunolocalization of mature peptides in shrimp hemocytes. In infected shrimps, bacteria and penaeidin distribution colocalized in the gills and the lymphoid organ that represented the main infected sites. Moreover, the shrimp immune response to infection involved massive hemocyte recruitment to infection sites where released penaeidin may participate in the isolation and elimination of the bacteria, We show that the ability of the shrimps to circumvent shrimp infections is closely related to a recovery phase based on the hematopoietic process. PMID:15095016

  20. Antimutagenicity and Antiproliferative Studies of Lipidic Extracts from White Shrimp (Litopenaeus vannamei)

    Science.gov (United States)

    Wilson-Sanchez, Griselda; Moreno-Félix, Carolina; Velazquez, Carlos; Plascencia-Jatomea, Maribel; Acosta, Anita; Machi-Lara, Lorena; Aldana-Madrid, María-Lourdes; Ezquerra-Brauer, Josafat-Marina; Robles-Zepeda, Ramón; Burgos-Hernandez, Armando

    2010-01-01

    An organic extract from fresh shrimp (Litopenaeus vannamei) was studied for antimutagenic and antiproliferative properties using Salmonella typhimurium tester strains TA98 and TA100 with metabolic activation (S9) and a cancer cell line (B-cell lymphoma), respectively. Shrimp extract was sequentially fractionated by thin layer chromatography (TLC) and each fraction was tested for antimutagenic and antiproliferative activities. Crude organic extracts obtained from shrimp reduced the number of revertants caused by aflatoxina B1, showing a dose-response type of relationship. Sequential TLC fractionation of the active extracts produced several antimutagenic and/or antiproliferative fractions. These results suggested that the lipid fraction of the tested species contained compounds with chemoprotective properties that reduce the mutagenicity of AFB1 and proliferation of a cancer cell line. PMID:21139845

  1. Molecular cloning and characterization of a threonine/serine protein kinase lvakt from Litopenaeus vannamei

    Science.gov (United States)

    Ruan, Lingwei; Liu, Rongdiao; Xu, Xun; Shi, Hong

    2014-07-01

    The phosphatidylinositol 3-kinase (PI3K)-AKT pathway is involved in various cellular functions, including anti-apoptosis, protein synthesis, glucose metabolism and cell cycling. However, the role of the PI3K-AKT pathway in crustaceans remains unclear. In the present study, we cloned and characterized the AKT gene lvakt from Litopenaeus vannamei. The 511-residue LVAKT was highly conserved; contained a PH domain, a catalytic domain and a hydrophobic domain; and was highly expressed in the heart and gills of L. vannamei. We found, using Real-Time Quantitative PCR (Q-PCR) analysis, that lvakt was up-regulated during early white spot syndrome virus (WSSV) infection. Moreover, the PI3K-specific inhibitor, LY294002, reduced viral gene transcription, implying that the PI3K-AKT pathway might be hijacked by WSSV. Our results therefore suggest that LVAKT may play an important role in the shrimp immune response against WSSV.

  2. Influence of Probiotics on the Growth and Digestive Enzyme Activity of White Pacific Shrimp (Litopenaeus vannamei)

    Institute of Scientific and Technical Information of China (English)

    G(o)mez R. Geovanny D.; MA Shen

    2008-01-01

    The influence of Bacillus probiotics on the digestive enzyme activity and the growth of Litopenaeus vannamei were de-termined in this study. The shrimp was treated with five percentages (1.5, 3.0, 4.5, 6.0 and 7.5) of probiotics (Bacillus spp.) supple-mented to the feed and cultured for 45d. The growth measured as the weight gain at the end of culturing was significantly (P<0.05) higher in probiotic-treated shrimps than that of the control (without receiving probiotics). Activities of protease and amylase, two digestive enzymes of the midgut gland and the intestine were significantly (P<0.05) higher in probiotic-treated shrimp than in the control.

  3. IMPACT OF PARASITISM BY GREGARINES (Nematopsis sp IN FARMING SHRIMP Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Francisco M. Guzmán-Sáenz

    2014-07-01

    Full Text Available Infestation of gregarines from genus Nematopsis in Pacific white shrimp Litopenaeus vannamei is commonly associated with a decrease in production and low weight, as well as a possible predisposition to viral infections. The goal of our past research was to evaluate the effect of the Nematopsis sp. gregarine parasitosis on the growth of farm-grown Litopenaeus vannamei shrimp. Four cages were built with PVC pipes and plastic screens with a 6mm light diameter, 1,7m in height, and 1,5m in width and length (2,23 m3, and were installed in a farm-growing area in La Pesca, Tamaulipas. Twenty-nine previously weighed shrimp, L. vannamei, were placed in each cage. These had a “2” (6 to 10 parasites per shrimp degree of infestation severity by Nematopsis sp. The shrimp in 2 cages were fed for 5 days with the farm feed and 6g/kg of sodium monensin. The shrimp in the remaining cages were fed with commercial feed. After 5 days, the shrimp in the 4 cages were fed with a balanced feed for 38 days. After this, the biomass of each cage was recorded. The shrimp in the control cages weighed an average of 7,12g at the beginning of research and 9,27g at the end, and the treated shrimp weighed 7,09g and 9,88g, respectively. The non-treated shrimp gained 2,5g and the treated shrimp gained 2,79g. This present research shows that the negative effect on the shrimp growth caused by Nematopsis sp. infestation can be reversed if the infected shrimp are treated against infestation with sodium monensin, which possesses a therapeutic effect that eliminated the infestation without affecting the shrimp growth.

  4. Essential roles of Cdc42 and MAPK in cadmium-induced apoptosis in Litopenaeus vannamei

    Energy Technology Data Exchange (ETDEWEB)

    Peng, Ting; Wang, Wei-Na, E-mail: weina63@aliyun.com; Gu, Mei-Mei; Xie, Chen-Ying; Xiao, Yu-Chao; Liu, Yuan; Wang, Lei

    2015-06-15

    Highlights: • Cd{sup 2+} induces Cdc42 and MAPKs pathway related gene of Litopenaeus vannamei up-regulation. • Reduction of THC, increase of ROS production and apoptotic cell rate were observed when the shrimps exposure to Cd{sup 2+}. • DsRNA-suppression of LvCdc42 and MAPKs during Cd{sup 2+} stress reduces the ROS production and apoptosis. • We conclude that LvCdc42 and MAPKs play key roles in Cd{sup 2+} stress responses of shrimps. - Abstract: Cadmium, one of the most toxic heavy metals in aquatic environments, has severe effects on marine invertebrates and fishes. The MAPK signaling pathway plays a vital role in stress responses of animals. The mitogen-activated protein kinase (MAPK) signaling pathway plays a vital role in animals’ stress responses, including mediation of apoptosis induced by the Rho GTPase Cdc42. However, there is limited knowledge about its function in shrimps, although disorders exacerbated by environmental stresses (including heavy metal pollution) have caused serious mortality in commercially cultured shrimps. Thus, we probed roles of Cdc42 in Litopenaeus vannamei shrimps (LvCdc42) during cadmium exposure by inhibiting its expression using dsRNA-mediated RNA interference. The treatment successfully reduced expression levels of MAPKs (including p38, JNK, and ERK). Cadmium exposure induced significant increases in expression levels of LvCdc42 and MAPKs, accompanied by reductions in total hemocyte counts (THC) and increases in apoptotic hemocyte ratios and ROS production. However, all of these responses were much weaker in LvCdc42-suppressed shrimps, in which mortality rates were higher than in controls. Our results suggest that the MAPK pathway plays a vital role in shrimps’ responses to Cd{sup 2+}. They also indicate that LvCdc42 in shrimps participates in its regulation, and thus plays key roles in ROS production, regulation of apoptosis and associated stress responses.

  5. The microsporidian Enterocytozoon hepatopenaei is not the cause of white feces syndrome in whiteleg shrimp Penaeus (Litopenaeus) vannamei

    OpenAIRE

    Tangprasittipap, Amornrat; Srisala, Jiraporn; Chouwdee, Saisunee; Somboon, Montagan; Chuchird, Niti; Limsuwan, Chalor; Srisuvan, Thinnarat; Flegel, Timothy W; Sritunyalucksana, Kallaya

    2013-01-01

    Background The microsporidian Enterocytozoon hepatopenaei was first described from Thailand in 2009 in farmed, indigenous giant tiger shrimp Penaeus (Penaeus) monodon. The natural reservoir for the parasite is still unknown. More recently, a microsporidian closely resembling it in morphology and tissue preference was found in Thai-farmed, exotic, whiteleg shrimp Penaeus (Litopenaeus) vannamei exhibiting white feces syndrome (WFS). Our objective was to compare the newly found pathogen with E. ...

  6. Feasibility of polyculture of blue shrimp Litopenaeus stylirostris and goldlined rabbitfish Siganus lineatus in a mesocosm system

    OpenAIRE

    Luong, Trung Cong; Hochard, Sebastien; Royer, Florence; Lemonnier, Hugues; Letourneur, Yves

    2014-01-01

    The study was conducted to (1) estimate the effects of polyculture of blue shrimp Litopenaeus stylirostris with goldlined rabbitfish Siganus lineatus on production, environmental quality and water and sediment metabolism and (2) to determine if blue shrimp and goldlined rabbitfish polyculture is possible. The experiment was carried out for 12 weeks in a mesocosm system that consisted of 12 circular outdoor mesocosm fiberglass tanks (1.7 m2, 1275 L water volume). Shrimp (2.9 g) were randomly s...

  7. Physiological response of the blue shrimp Litopenaeus stylirostris to short-term confinement on a pond bottom

    OpenAIRE

    Mugnier, Chantal; Lemonnier, Hugues; Legrand, Alexandre

    2006-01-01

    The blue shrimp Litopenaeus stylirostris was exposed to pond sediment for 48 h and its physiological response was studied in relation to molt stage. Shrimp were maintained in cages within a semi-intensive pond after 4 months of rearing. Hypoosmoregulatory capacity (hypo-OC), magnesium ions (Mg ions), glucose, total proteins and oxyhaemocyanin were assayed in haemolymph. An increase of 370% to 500% in Mg ions concentration and 200% to 266% in glucose concentration, and a decrease in hypo-OC in...

  8. Experimental evaluation of co-culture of juvenile sea cucumbers, Holothuria scabra (Jaeger), with juvenile blue shrimp, Litopenaeus stylirostris (Stimpson)

    OpenAIRE

    Purcell, S.; Patrois, Jacques; Fraisse, N

    2006-01-01

    The co-culture of juvenile sea cucumber Holothuria scabra (Jaeger), or 'sandfish', with juvenile blue shrimp Litopenaeus stylirostris (Stimpson) was tested by growing groups in co-culture and monoculture for 3 weeks in tanks with enriched sand substratum. Feed was supplied on trays, accessible only to shrimp. Survival of shrimp and sandfish was high in all treatments (73-100%). Growth of shrimp did not differ between monoculture and co-culture, but sandfish grew significantly slower in co-cul...

  9. Effect of probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress of Litopenaeus stylirostris under Vibrio nigripulchritudo challenge

    OpenAIRE

    Castex, Mathieu; Lemaire, Pierrette; Wabete, Nelly; Chim, Liet

    2010-01-01

    Antioxidant defences and induced oxidative stress tissue damage of the blue shrimp Litopenaeus stylirostris, under challenge with Vibrio nigriputchritudo, were investigated for a 72-h period. For this purpose, L stylirostris were first infected by immersion with pathogenic V. nigripulchritudo strain SFn1 and then antioxidant defences: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (Gpx), Total antioxidant status (TAS), glutathiones and induced tissue damage (MDA and carbon...

  10. A soft technology to improve survival and reproductive performance of Litopenaeus stylirostris by counterbalancing physiological disturbances associated with handling stress

    OpenAIRE

    Wabete, Nelly; Chim, Liet; Pham, Dominique; Lemaire, Pierrette; Massabuau, J

    2006-01-01

    The consequences of handling stress (fishing, transfer, eyestalk ablation) on shrimp broodstock are poorly documented. The weakness of farmed shrimp, Litopenaeus stylirostris, during winter is a major problem in New Caledonia, because of seasonal climate (tropical-sub-temperate). The transfer of broodstock in winter from earthen outdoor ponds to indoor maturation tanks in the hatchery (T=20 degrees C, Salinity=35 parts per thousand, fed shrimp) usually leads, after 48 h, to high mortality (up...

  11. Metal and metalloid bioaccumulation in the Pacific blue shrimp Litopenaeus stylirostris (Stimpson) from New Caledonia: laboratory and field studies

    OpenAIRE

    Metian, Marc; Eltayeb, Mohamed M.; Hédouin, Laëtitia; Lacoue-Labarthe, Thomas; Teyssié, Jean-Louis; Mugnier, Chantal; Bustamante, Paco; Warnau, Michel

    2010-01-01

    The present work aimed at better understanding metal and metalloid bioaccumulation in the edible Pacific blue shrimp Litopenaeus stylirostris, using both laboratory and field approaches. In the laboratory, the bioaccumulation kinetics of Ag, Cd, Co, Cr, and Zn have been investigated in shrimp exposed via seawater and food, using the corresponding gamma-emitting radiotracers (Ag-110m, Cd-109, Co-57, Cr-51, and Zn-65) and highly sensitive nuclear detection techniques. Results showed that hepato...

  12. Novel transcriptome assembly and improved annotation of the whiteleg shrimp (Litopenaeus vannamei), a dominant crustacean in global seafood mariculture

    OpenAIRE

    Ghaffari, Noushin; Sanchez-Flores, Alejandro; Doan, Ryan; Garcia-Orozco, Karina D.; Chen, Patricia L.; Ochoa-Leyva, Adrian; Lopez-Zavala, Alonso A.; Carrasco, J. Salvador; Hong, Chris; Brieba, Luis G.; Rudiño-Piñera, Enrique; Blood, Philip D; Jason E. Sawyer; Charles D Johnson; Dindot, Scott V.

    2014-01-01

    We present a new transcriptome assembly of the Pacific whiteleg shrimp (Litopenaeus vannamei), the species most farmed for human consumption. Its functional annotation, a substantial improvement over previous ones, is provided freely. RNA-Seq with Illumina HiSeq technology was used to analyze samples extracted from shrimp abdominal muscle, hepatopancreas, gills and pleopods. We used the Trinity and Trinotate software suites for transcriptome assembly and annotation, respectively. The quality ...

  13. The complete mitochondrial genomes of the yellowleg shrimp Farfantepenaeus californiensis and the blue shrimp Litopenaeus stylirostris (Crustacea: Decapoda).

    Science.gov (United States)

    Peregrino-Uriarte, Alma B; Varela-Romero, Alejandro; Muhlia-Almazán, Adriana; Anduro-Corona, Iván; Vega-Heredia, Sarahí; Gutiérrez-Millán, Luis E; De la Rosa-Vélez, Jorge; Yepiz-Plascencia, Gloria

    2009-03-01

    Mitochondria play key roles in many cellular processes. Description of penaeid shrimp genes, including mitochondrial genomes are fairly recent and some are focusing on commercially important shrimp as the Pacific shrimp Litopenaeus vannamei that is being used for aquaculture not only in America, but also in Asia. Much less is known about other Pacific shrimp such as the yellowleg shrimp Farfantepenaeus californiensis and the blue shrimp Litopenaeus stylirostris. We report the complete mitogenomes from these last two Pacific shrimp species. Long DNA fragments were obtained by PCR and then used to get internal fragments for sequencing. The complete F. californiensis and L. stylirostris mtDNAs are 15,975 and 15,988 bp long, containing the 37 common sequences and a control region of 990 and 999 bp, respectively. The gene order is identical to that of the tiger shrimp Penaeus monodon. Secondary structures for the 22 tRNAs are proposed and phylogenetic relationships for selected complete crustacean mitogenomes are included. Phylogenomic relationships among five shrimp show strong statistical support for the monophyly of the genus across the analysis. Litopenaeus species define a clade, with close relationship to Farfantepenaeus, and both clade with the sister group of Penaeus and Fenneropenaeus. PMID:20403743

  14. Distribución, ecología y caracterzación in situ de la vid silvestre en la Reserva de la Biosfera de Urdaibai (Bizkaia

    Directory of Open Access Journals (Sweden)

    Aranburu Albizuri, A., Gallardo Cano, A., López Martínez, M.A., Pérez Izquierdo, M.A., Ocete Rubio, R.

    2009-01-01

    Se incide sobre la necesidad de la conservación in situ y ex situ de este recurso fitogenético. Como Urdaibai ha sido una zona tradicional de producción de chacolí, se señalan posibles lineas de investigación, tanto filogenéticas como para el empleo de material silvestre en la mejora de las variedades cultivadas.

  15. Serological surveillance on South American wild canids for Neospora caninum Sorologia para Neospora caninum em canídeos silvestres sul-americanos

    Directory of Open Access Journals (Sweden)

    C.B. Melo

    2002-08-01

    Full Text Available Esse trabalho objetivou pesquisar anticorpos para Neospora caninum em amostras de soro de canídeos silvestres sul-americanos pela técnica de imunofluorescência indireta (IFI. As amostras foram coletadas de 48 lobos-guará (Chrysocyon brachyurus, provenientes de zoológicos e de vida livre e de dois cachorros-do-mato (Cerdocyon thous. Anticorpos para N. caninum não foram detectados nas amostras testadas.

  16. Estudio preliminar de especies de micobacterias en primates colombianos no humanos en cautiverio en dos centros de rescate de fauna silvestre de Bogotá

    Directory of Open Access Journals (Sweden)

    Barragán Fonseca Karol Bibiana

    2005-07-01

    Full Text Available Se determinó la presencia de diferentes especies de micobacterias en 83 primates colombianos en cautiverio en dos centros de rescate de fauna silvestre de Bogotá. Se evaluaron 166 muestras de sangre y lavado gástrico mediante amplificación por PCR-spoligotyping para micobacterias del
    complejo tuberculosis y Hsp65-PRA para micobacterias no  tuberculosas (MNT. Se obtuvo una prevalencia del 2,4% para micobacterias del complejo Mycobacterium tuberculosis, encontrando un Saimiri sciureus infectado con Mycobacterium microti tipo vole y un Saguinus leucopus infectado con
    Mycobacterium africanum, siendo el primer reporte de estas micobacterias para primates del Nuevo Mundo. La prevalencia para MNT fue de 7,2% perteneciendo a las especies Mycobacterium phlei, M. terrae, M. vaccae, M. flavescens, M. flavescens y M. zsulgaii, algunas de estas, patógenos oportunistas. No existe una diferencia a nivel descriptivo en cuanto a la edad, al sexo y al tipo de entrega de los individuos infectados con MNT y micobacterias tuberculosas con respecto a los individuos que no las presentaron. Los primates del Nuevo Mundo pueden ser portadores sanos de micobacterias tuberculosas lo que representa un riesgo para la salud humana, y con ciertas especies de micobacterias, podría ser un riesgo para las poblaciones de primates silvestres en caso de que estos primates sean liberados en el medio silvestre. Este estudio es relevante para el país, ya que integra conceptos médicos tanto epidemiológicos como microbiológicos, con aspectos relacionados con la conservación de la fauna silvestre.

  17. Infecção natural de roedores silvestres pelo Schistosoma mansoni Natural infection of sylvatic rodents by Schistosoma mansoni

    Directory of Open Access Journals (Sweden)

    Tânia Maria Correa Silva

    1989-06-01

    Full Text Available No município baiano de Planalto, 47% dos roedores silvestres capturados (Nectomys estavam infectados pelo Schistosoma mansoni, enquanto a prevalência desta infecção na população humana da área era de 3,26%. Os roedores habitam zonas peridomiciliares, têm hábitos aquáticos e eliminam ovos viáveis do S. mansoni. Albergam número variável de vermes e formam granulomas periovulares pequenos, principalmente no fígado e intestinos, sem fibrose hepática importante ou sinais de hipertensão porta. A deposição maior de ovos se faz a nível do intestino, sobretudo do jejuno, com passagem de grande número de ovos para as fezes. Miracídios isolados a partir dos ovos retirados dos roedores infectaram normalmente a Biomphalaria glabrata, com eliminação de cercárias, com as quais se provocou infecção no camundongo branco, em tudo semelhante aquelas causadas por outras cepas de origem humana. Também camundongos que foram deixados em contacto com as águas infestadas pelos roedores silvestres se infectaram facilmente, atestando o alto grau de transmissibilidade da área. Conclui-se que os roedores silvestres de planalto toleram bem a infecção esquistossomótica natural, são bons eliminadores de ovos viáveis do S. mansoni, estão infectados por uma cepa semelhante a que infecta o homem e podem ter um papel na manutenção do ciclo vital do S. mansoni na área estudada.In Planalto, a small locality in the interior of the Bahia state, Brazil, 47% of sylvatic rodents were found to be naturally infected with Schistosoma mansoni, whereas the prevalence of the infection in the inhabitants of the area was 3.26%. The rodents (Nectomys live near the houses, in contact with water passing viable schistosome eggs in the stools. Worm burden is variable amongst such rodents. Periovular granulomas are small, especially in liver and intestines, and hepatic fibrosis is mild or absent, with no morphological evidence of portal hypertension being noted

  18. Florescimento e frutificação do maracujazeiro silvestre Passiflora setacea D. C. cultivado em Jaboticabal, SP

    Directory of Open Access Journals (Sweden)

    Elma Machado Ataíde

    2012-06-01

    Full Text Available Muitas espécies de Passiflora apresentam comportamento florífero diferente durante o ano e, consequentemente, com variações nas produções e períodos de colheitas, como observado para o maracujazeiro-amarelo (Passiflora edulis sims. Estudo com espécies silvestre constitui-se numa importante alternativa para usos de novos genótipos no melhoramento das espécies cultivadas comercialmente, em especial, a Passiflora setacea, devido ao grande potencial de mercado; contudo, essa espécie é pouco estudada, principalmente em relação à propagação, germinação, floração e condições de armazenamento. Assim, o objetivo deste trabalho foi avaliar o florescimento e a frutificação do maracujazeiro silvestre Passiflora setacea cultivado em Jaboticabal-SP. A floração e a frutificação foram avaliadas pelo percentual de frutificação através da polinização natural e artificial das flores, realizado no período de agosto a outubro. As características físicas dos frutos foram avaliadas através dos seguintes parâmetros: a massa do fruto (g; b diâmetro longitudinal do fruto (cm; c diâmetro transversal do fruto (cm; d espessura da casca (cm; e rendimento de polpa (%. A característica química foi determinada por meio do teor de sólidos solúveis (SS. Verificou-se que a Passiflora setacea apresentou precocidade de floração em relação ao maracujazeiro-amarelo, com florescimento durante o ano todo, nas condições de Jaboticabal-SP. Observou-se que essa espécie possui bom nível de tolerância às doenças foliares, resistência à morte precoce e apresenta diferentes níveis de compatibilidade entre as plantas, possibilitando a essas características serem utilizadas em programas de melhoramento genético. Constatou-se ainda que as características físicas encontradas nos frutos atendem às exigências da indústria.

  19. Tráfico y tenencia ilegal de fauna silvestre en el departamento de Boyacá

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    Alejandro Cabrejo Bello

    2010-10-01

    Full Text Available Con el fin de establecer cuáles son los principales grupos taxonómicos, suparticipación y las ciudades con mayores índices de tráfico y tenencia ilegal de vida silvestre en el departamento de Boyacá, jurisdicción de la Corporación Autónoma Regional de Boyacá- Corpoboyacá, se tomaron reportes de la base de datos de dicha entidad entre los años 2004 y 2008. Se hallaron registros de 398 individuos decomisados por parte de la autoridad ambiental y otros por entregas voluntarias. El grupo con mayor incidencia fue el de las aves con 58%,seguido por los reptiles con 26% y en tercer lugar los mamíferos con el 16% de los registros. Se observó que las tres principales ciudades del departamento (Tunja, Duitama y Sogamoso reportan las frecuencias más elevadas de incautaciones. Puerto Boyacá, también registra un número elevado de decomisos. Dentro de los tres grupos mencionados, las especies más afectadas correspondena los órdenes Psitaciformes, Primates y Testudínata, por lo cual merecen principal atención. Se recomienda además, implementar planes de conservación y emprender actividades de educación ambiental encaminadas a la utilización sostenible de la fauna en general.

  20. Reflexiones sobre el impacto y manejo de la caza de mamíferos silvestres en los bosques tropicales

    Directory of Open Access Journals (Sweden)

    J.E. Fa

    2013-01-01

    Full Text Available comunidades autóctonas de las selvas en América, África y Asia, la fauna es una fuente importante de proteína animal y también de ingresos deri- vados de la venta de los animales cazados. La caza comercial de fauna selvática, junto con las demandas impuestas por el intenso crecimiento de la población humana en estos ecosistemas, supone sin embargo una amenaza para la viabilidad de muchas poblaciones de mamíferos silvestres. Este impacto pone en riesgo la biodiversidad de estos ecosistemas y puede reducir las fuentes de proteínas para las poblaciones rurales. En este trabajo se presenta un análisis del volumen de carne de monte extraída en selvas tropicales a escala mundial, y se examina su importancia en las dietas y economías de las comunidades rurales y urbanas en estas regiones. Se realiza una breve revisión sobre el estado de conservación de aquellas especies afectadas por la caza, y de los procesos que han contribuido a su situación actual. Se concluye con un análisis de las soluciones propuestas para minimizar el conflicto entre la protección de la biodiversidad y las necesidades de las poblaciones humanas dependientes de ella en las selvas tropicales.

  1. MAMÍFEROS INVASORES EN LA PATAGONIA ARGENTINA: EL CONEJO SILVESTRE EUROPEO COMO CASO EMBLEMÁTICO

    Directory of Open Access Journals (Sweden)

    Never Antonio Bonino Vassallo

    2010-04-01

    Full Text Available Se brinda una reseña de los estudios biológicos y ecológicos realizados sobre el conejo silvestre europeo (Oryctolagus cuniculus, tal vez el más emblemático de los mamíferos exóticos introducidos en Argentina. En el sector continental argentino el conejo se encuentra establecido, y en proceso de dispersión, principalmente en las provincias de Mendoza y Neuquén (NO de la Patagonia. En esta región, el conejo ha demostrado tener un efecto negativo sobre la economía regional, especialmente en áreas ganaderas y en plantaciones comerciales de árboles frutales y maderables. No existen programas oficiales para controlar la dispersión geográfica y/o el impacto de esta especie exótica. La gente que se siente perjudicada por el conejo recurre a intentos individuales para controlar sus efectos negativos. Es necesario implementar monitoreos regulares de la dispersión del conejo para la detección temprana y el manejo de poblaciones recién establecidas; además, se requieren estudios comparativos tendientes a determinar los métodos más adecuados para controlar el impacto del conejo.

  2. Development of EST-SSR markers by data mining in three species of shrimp: Litopenaeus vannamei, Litopenaeus stylirostris, and Trachypenaeus birdy

    OpenAIRE

    Pérez, F; Ortiz, J.; Zhinaula, M.; Gonzabay, C.; Calderón, J.; Volckaert, F.A.M.J.

    2005-01-01

    We report on the data mining of publicly available Litopenaeus vannamei expressed sequence tags (ESTs) to generate simple sequence repeat (SSRs) markers and on their transferability between related Penaeid shrimp species. Repeat motifs were found in 3.8% of the evaluated ESTs at a frequency of one repeat every 7.8 kb of sequence data. A total of 206 primer pairs were designed, and 112 loci were amplified with the highest success in L. vannamei. A high percentage (69%) of EST-SSRs were transfe...

  3. Carotenoides totais em resíduos do camarão Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Larissa Mont'Alverne Jucá Seabra

    2014-02-01

    Full Text Available Considerando-se o possível aproveitamento dos resíduos, provenientes do beneficiamento do camarão, realizouse, neste trabalho, a determinação da concentração de carotenoides totais dos resíduos do camarão Litopenaeus vannamei e da farinha obtida após secagem. As análises foram realizadas nos resíduos in natura e na farinha recémprocessada (dia 0 e aos 60, 120 e 180 dias de armazenamento sob congelamento. Os resíduos frescos apresentaram, no dia 0, teores de 42,74 µg/g de carotenoides totais e, a farinha recém-processada, de 98,51 µg/g. Após 180 dias de armazenamento, sob congelamento, os teores de carotenoides totais diminuíram significativamente, quando comparados com os do dia 0 (p < 0,05.

  4. Effects of Hypoxia on Dopamine Concentration and the Immune Response of White Shrimp (Litopenaeus vannamei)

    Institute of Scientific and Technical Information of China (English)

    HU Fawen; PAN Luqing; JING Futao

    2009-01-01

    Effects of hypoxia on the dopamine concentration and the immune response of White Shrimp Litopenaeus vannamei were studied. The results showed that hypoxia had significant effects on the concentration of dopamine (DA) in the haemolymph, haemocyte count, phenoloxidase activity, phagocytic activity of haemocytes and bacteriolytic and antibacterial activity in the haemolvmph (P<0.05). The concentration of the dopamine in haemolymph reached its maximum in the 3.0 and 1.5mgL-1 DO groups at 12h and 6h, and then returned to normal after 24h and 12h, respectively. All immune parameters decreased with the reduction of dissolved oxygen. Total haemocyte count (THC), the hyaline cells and semi-granular cells in the 3.0mgL-1 DO group became stable after 12h,while granular cells did so after 24h, The THC and different haemocyte count (DHC) in the 1.5mgL-1 DO group became stable alter 24h. Phenoloxidase activity and bacteriolytic activity in the 3.0 and 1.5mgL-1 DO groups reached their stable levels after 24 h and 12 h respectively, while phagocytic activity and antibacterial activity became stable after 24 and 12, and 36 and 24 It, respec-tively. It was also indicated that the changes of dopamine concentrations in haemolymph, haemocyte count and phenoloxidase activ-ity were obviously related to the exposure time under hypoxic conditions.

  5. Antimutagenic Compounds of White Shrimp (Litopenaeus vannamei): Isolation and Structural Elucidation

    Science.gov (United States)

    López-Saiz, Carmen-María; Hernández, Javier; Cinco-Moroyoqui, Francisco-Javier; Velázquez, Carlos; Ocaño-Higuera, Víctor-Manuel; Plascencia-Jatomea, Maribel; Robles-Sánchez, Maribel; Machi-Lara, Lorena; Burgos-Hernández, Armando

    2016-01-01

    According to the World Health Organization, cancer is the main cause of mortality worldwide; thus, the search of chemopreventive compounds to prevent the disease has become a priority. White shrimp (Litopenaeus vannamei) has been reported as a source of compounds with chemopreventive activities. In this study, shrimp lipids were extracted and then fractionated in order to isolate those compounds responsible for the antimutagenic activity. The antimutagenic activity was assessed by the inhibition of the mutagenic effect of aflatoxin B1 on TA98 and TA100 Salmonella tester strains using the Ames test. Methanolic fraction was responsible for the highest antimutagenic activity (95.6 and 95.9% for TA98 and TA100, resp.) and was further separated into fifteen different subfractions (M1–M15). Fraction M8 exerted the highest inhibition of AFB1 mutation (96.5 and 101.6% for TA98 and TA100, resp.) and, after further fractionation, four subfractions M8a, M8b, M8c, and M8d were obtained. Data from 1H and 13C NMR, and mass spectrometry analysis of fraction M8a (the one with the highest antimutagenic activity), suggest that the compound responsible for its antimutagenicity is an apocarotenoid. PMID:27006678

  6. Heritability of body weight and resistance to ammonia in the Pacific white shrimp Litopenaeus vannamei juveniles

    Science.gov (United States)

    Li, Wenjia; Lu, Xia; Luan, Sheng; Luo, Kun; Sui, Juan; Kong, Jie

    2016-01-01

    Ammonia, toxic to aquaculture organisms, represents a potential problem in aquaculture systems, and the situation is exacerbated in closed and intensive shrimp farming operations, expecially for Litopenaeus vannamei. Assessing the potential for the genetic improvement of resistance to ammonia in L. vannamei requires knowledge of the genetic parameters of this trait. The heritability of resistance to ammonia was estimated using two descriptors in the present study: the survival time (ST) and the survival status at half lethal time (SS50) for each individual under high ammonia challenge. The heritability of ST and SS50 were low (0.154 4±0.044 6 and 0.147 5±0.040 0, respectively), but they were both significantly different from zero (P 0.05), suggesting that ST and SS50 could be used as suitable indicators for resistance to ammonia. There were also positive phenotypic and genetic correlation between resistance to ammonia and body weight, which means that resistance to ammonia can be enhanced by the improvement of husbandry practices that increase the body weight. The results from the present study suggest that the selection for higher body weight does not have any negative consequences for resistance to ammonia. In addition to quantitative genetics, tools from molecular genetics can be applied to selective breeding programs to improve the efficiency of selection for traits with low heritability.

  7. Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei

    Science.gov (United States)

    Yu, Yang; Zhang, Xiaojun; Liu, Jingwen; Li, Fuhua; Huang, Hao; Li, Yijun; Liu, Xiaolin; Xiang, Jianhai

    2015-01-01

    Selective breeding of the Pacific white shrimp Litopenaeus vannamei during the last decade has produced new varieties exhibiting high growth rates and disease resistance. However, the identification of new varieties of shrimps from their phenotypic characters is difficult. This study introduces a new approach for identifying varieties of shrimps using molecular markers of microsatellites and mitochondrial control region sequences. The method was employed to identify a new selected variety, Kehai No. 1 (KH-1), from three representative stocks (control group): Zhengda; Tongwei; and a stock collected from Fujian Province, which is now cultured in mainland China. By pooled genotyping of KH-1 and the control group, five microsatellites showing differences between KH-1 and the control group were screened out. Individual genotyping data confirmed the results from pooled genotyping. The genotyping data for the five microsatellites were applied to the assignment analysis of the KH-1 group and the control group using the partial Bayesian assignment method in GENECLASS2. By sequencing the mitochondrial control regions of individuals from the KH-1 and control group, four haplotypes were observed in the KH-1 group, whereas 14 haplotypes were obtained in the control group. By combining the microsatellite assignment analysis with mitochondrial control region analysis, the average accuracy of identification of individuals in the KH-1 group and control group reached 89%. The five selected microsatellite loci and mitochondrial control region sequences were highly polymorphic and could be used to distinguish new selected varieties of L. vannamei from other populations cultured in China.

  8. Modified extensive pond culture of Litopenaeus vannamei for sustainable shrimp culture in the Philippines

    Directory of Open Access Journals (Sweden)

    Cecilia J. Jaspe

    2011-06-01

    Full Text Available The shrimp culture industry provides huge revenues to most aquaculture producing countries,but it is also beset with problems that hamper its sustainability. In the present study, we described amodified extensive pond culture method for white shrimp, Litopenaeus vannamei in the Philippines duringthe wet and dry months. One hectare earthen ponds were prepared and added with organic and/orinorganic fertilizers to stimulate natural food production. The ponds were stocked with L. vannameipostlarvae (PL at a density of 4 PL m-2. A zero-water exchange system of pond management was doneduring the first two months of culture followed by a bi-weekly water exchange until harvest. No artificialfeeding was given during the culture period, instead, the ponds were applied with inorganic fertilizerevery month to ensure continuous supply of natural food. During the culture period, the shrimpsappeared healthy and no disease outbreaks were observed. All the physico-chemical parameters of thewater in the pond were within the optimum range required for shrimp farming and the phytoplanktonpopulation was predominantly green microalgae (Chlorophyta. The shrimps were harvested after 3 to3.5 months of culture or when they reached an average body weight of 13-15 g, with moderate to highsurvival rates depending on the prevailing climatic conditions.

  9. Molecular characterization of penaeidins from two Atlantic Brazilian shrimp species, Farfantepenaeus paulensis and Litopenaeus schmitti.

    Science.gov (United States)

    Barracco, Margherita Anna; de Lorgeril, Julien; Gueguen, Yannick; Bachère, Evelyne

    2005-09-01

    We report here the molecular cloning of new members of the penaeidin family from two Atlantic penaeids from Brazil, Litopenaeus schmitti and Farfantepenaeus paulensis. The presence of penaeidins in the granular hemocytes of both shrimps was first evidenced by immunofluorescence, using polyclonal antibodies raised against L. vannamei penaeidin Litvan PEN3-1. cDNAs from the hemocytes of both Brazilian species were obtained by reverse transcription and the sequences encoding penaeidins were amplified by PCR, using primers based on penaeidin consensus sequences. Five penaeidin clones were obtained. According to the international penaeidin classification (PenBase, http://www.penbase.immunaqua.com), the deduced amino acid sequences of two clones from L. schmitti and two from F. paulensis belong to the PEN2 subgroup and one clone from L. schmitti to the PEN4 subgroup of penaeidins. Surprisingly, no penaeidin from the PEN3 subgroup was obtained in both shrimp species, even though this subgroup appears to be the most commonly expressed in the hemocytes of penaeids.

  10. Genetic variation in a closed line of the white shrimp Litopenaeus vannamei (Penaeidae

    Directory of Open Access Journals (Sweden)

    Eloize Luvesuto

    2007-01-01

    Full Text Available The culture of the marine shrimp Litopenaeus vannamei has recently boosted the Brazilian shrimp industry. However, it is well known that selection methods based solely on phenotypic characteristics, a reduced number of breeders and the practice of inbreeding may promote a significant raise in the genetic similarity of the captive populations, leading to greater disease susceptibility and impairing both the growth and final size of the shrimps. We used four microsatellite loci to investigate genetic variation in three generations (F5, F6 and F7 of a closed and reared L. vannamei lineage. Although an accentuated heterozygosis deficit was detected, we also observed that the captive propagation of this lineage did not lead to a significant loss of genetic variability over the three generations studied. One possible reason for this is that the breeding conditions of this lineage were good enough to prevent any significant loss of genetic variability. However, three generations may have been insufficient to produce detectable changes in genetic frequencies in the loci studied. Alternatively, the microsatellite loci may have been non-neutral (biased and related to the conditions in which the shrimps were kept, resulting in a similar allele pool in respect to these four microsatellites over the three generations studied. Any generalizations regarding microsatellite variation in closed shrimp lines may thus be incomplete and should be carefully analyzed.

  11. Standardization of sodium metabisulfite solution concentrations and immersion time for farmed shrimp Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Luciana Trigueiro de Andrade

    2015-03-01

    Full Text Available Sodium metabisulfite is the main additive used in the prevention of melanosis in shrimp; however, it has currently been employed with great variation in concentration by producers. Thus, the aim of the present study was to determine the correlation between the concentration of the sodium metabisulfite solution and immersion time of the whole shrimp to obtain the concentration of sulfur dioxide (SO2 in the edible muscle of farmed shrimp (Litopenaeus vannamei in accordance with the limit established by law. For this, solutions of sodium metabisulfite at different concentrations (1%, 2 %, 3 %, 4% and 5% were prepared and samples of L. vannamei shrimp (100g were immersed during 10, 20 or 30 minutes at temperature of 7°C. For all treatment assayed the concentration of SO2 was determined in the edible muscle of farmed shrimp (L. vannamei. The results show that for the conditions used in this study, the correlations were linear, with significant increase (P<0.05 in the SO2 concentration in the edible muscle of shrimps both increasing sodium metabisulfite concentration as increasing immersion times, suggesting the immersion of shrimps in a 3% solution for a time of 13 minutes in order to obtain SO2 concentration of 100ppm in its edible muscle in accordance with Brazilian legislation

  12. Comparison of the Respiratory Metabolism of Juvenile Litopenaeus vannamei Cultured in Seawater and Freshwater

    Institute of Scientific and Technical Information of China (English)

    DING Sen; WANG Fang; DONG Shuanglin; LI Ying

    2014-01-01

    Litopenaeus vannamei, a euryhaline species, can be cultured at a wide range of salinities. The emergence of freshwater pond-culture of L. vannamei is an important prelude to the continued development of shrimp culture in China. In this study, we com-pared the respiratory metabolism of juvenile L. vannamei cultured in freshwater and saltwater by measuring their oxygen consump-tion rate (OCR), ammonium-type nitrogen excretion rate (AER) and pyruvate kinase (PK) and lactate dehydrogenase (LDH) activi-ties at different molting stages in order to physiecologically characterize juvenile L. vannamei under freshwater conditions. The re-sults showed that OCR was significantly higher in saltwater than in freshwater at all stages of molting cycle. However, variation of OCR among molting stages in saltwater was similar with that in freshwater, and the highest OCR was observed at post-molting stage. At all stages of molting cycle, AER was significantly higher in freshwater than in saltwater, and the highest was observed at post-molting stage. The activity of PK was significantly higher in saltwater than in freshwater. Conversely, the activity of LDH was higher in freshwater than in saltwater in general. Significant variation of PK and LDH activities in molting cycle was observed in saltwater and freshwater. The results indicated that aerobic metabolism of juvenile L. vannamei was more active in saltwater than in freshwater;while its protein metabolism was more active in freshwater than in saltwater.

  13. Comparison of the respiratory metabolism of juvenile Litopenaeus vannamei cultured in seawater and freshwater

    Science.gov (United States)

    Ding, Sen; Wang, Fang; Dong, Shuanglin; Li, Ying

    2013-11-01

    Litopenaeus vannamei, a euryhaline species, can be cultured at a wide range of salinities. The emergence of freshwater pond-culture of L. vannamei is an important prelude to the continued development of shrimp culture in China. In this study, we compared the respiratory metabolism of juvenile L. vannamei cultured in freshwater and saltwater by measuring their oxygen consumption rate (OCR), ammonium-type nitrogen excretion rate (AER) and pyruvate kinase (PK) and lactate dehydrogenase (LDH) activities at different molting stages in order to physiecologically characterize juvenile L. vannamei under freshwater conditions. The results showed that OCR was significantly higher in saltwater than in freshwater at all stages of molting cycle. However, variation of OCR among molting stages in saltwater was similar with that in freshwater, and the highest OCR was observed at post-molting stage. At all stages of molting cycle, AER was significantly higher in freshwater than in saltwater, and the highest was observed at post-molting stage. The activity of PK was significantly higher in saltwater than in freshwater. Conversely, the activity of LDH was higher in freshwater than in saltwater in general. Significant variation of PK and LDH activities in molting cycle was observed in saltwater and freshwater. The results indicated that aerobic metabolism of juvenile L. vannamei was more active in saltwater than in freshwater; while its protein metabolism was more active in freshwater than in saltwater.

  14. Evaluation of different lipid sources in diet of pacific white shrimp Litopenaeus vannamei at low salinity

    Directory of Open Access Journals (Sweden)

    Ke Chen

    2015-11-01

    Full Text Available Litopenaeus vannamei (1.98 ± 0.28 g were fed diets containing soybean oil (SBO, beef tallow (BFT, fish oil (FIO, linseed oil (LNO, and an equal combination of SBO + BFT + FIO (SBF or SBO + BFT + LNO (SBL as dietary lipid source respectively for 8 weeks at low salinity of 3‰. The shrimp fed the SBL diet had the highest weight gain and survival rate. The whole body fatty acid composition including the EPA and DHA of L. vannamei generally reflected the composition of dietary fatty acids with the highest DHA and EPA found in L. vannamei fed FIO. The activities of fatty acid synthetase, acyl-CoA, diacylgycerol acyltransferase 2, elongase of long-chain fatty acids family member 6, Δ5 and Δ6 fatty acid desaturases of shrimp fed SBL were significantly lower than those fed BFT. The results indicated that fish oil could not be the only lipid source for L. vannamei cultured at low salinity, and the shrimp fed non-fish oil diet with a suitable proportion of PUFAs could obtain the same growth and survival rate as those fed diets with fish oil.

  15. Effect of salinity on regulation mechanism of neuroendocrine-immunoregulatory network in Litopenaeus vannamei.

    Science.gov (United States)

    Zhao, Qun; Pan, Luqing; Ren, Qin; Wang, Lin; Miao, Jingjing

    2016-02-01

    The effects of low salinity (transferred from 31‰ to 26‰, 21‰, and 16‰) on the regulation pathways of neuroendocrine-immunoregulatory network were investigated in Litopenaeus vannamei. The results showed that the hormones (corticotrophin-releasing hormone, adrenocorticotropic hormone) and biogenic amines (dopamine, noradrenaline, 5-hydroxytryptamine) concentrations in lower salinity groups increased significantly within 12 h. The gene expression of biogenic amine receptors showed that dopamine receptor D4 and α2 adrenergic receptor in lower salinity groups decreased significantly within 12 h, whereas the 5-HT7 receptor significantly increased within 1d. The second messenger synthetases (adenylyl cyclase, phospholipase C) and the second messengers (cyclic adenosine monophosphate, cyclic guanosine monophosphate) of lower salinity groups shared a similar trend in which adenylyl cyclase and cyclic adenosine monophosphate reached the maximum at 12 h, whereas phospholipase C and cyclic guanosine monophosphate reached the minimum. The immune parameters (total hemocyte count, phenoloxidase activity, phagocytic activity, crustin expression, antibacterial activity, C-type lectin expression, hemagglutinating activity) in lower salinity groups decreased significantly within 12 h. Except for the total hemocyte count, all the parameters recovered to the control levels afterwards. Therefore, it may be concluded that the neuroendocrine-immunoregulatory network plays a principal role in adapting to salinity changes as the main center for sensing the stress and causes immune response in L. vannamei. PMID:26766179

  16. Transcriptome analysis of the unfolded protein response in hemocytes of Litopenaeus vannamei.

    Science.gov (United States)

    Chen, Yi-Hong; Yuan, Feng-Hua; Bi, Hai-Tao; Zhang, Ze-Zhi; Yue, Hai-Tao; Yuan, Kai; Chen, Yong-Gui; Wen, Shao-Ping; He, Jian-Guo

    2016-07-01

    In this study, Litopenaeus vannamei was injected with double-stranded RNA (dsRNA) against L. vannamei immunoglobulin heavy chain binding protein (LvBip) to activating UPR in the hemocytes, shirmps injected dsRNA against enhanced green fluorescence protein (eGFP) as control group. And genes expression in hemocytes of then were analyzed using Illumina Hiseq 2500 (PE100). By comparing the analyzed results, 1418 unigenes were significantly upregulated, and 596 unigenes were significantly down-regulated upon UPR. Analysis of the differentially expressed genes against known databases indicated that the distribution of gene pathways between the upregulated and down-regulated genes were substantially different. A total of 208 genes of UPR system were obtained, and 69 of them were differentially expressed between the two groups. Results also showed that L. vannamei UPR was involved in various metabolic processes, such as glycometabolism, lipid metabolism, amino acid metabolism, and nucleic acid metabolism. In addition, UPR was emgaged in immune-assicoated signaling pathways, such as NF-κB signaling pathway, NOD-like receptor signaling pathway, Hippo signaling pathway, p38 MAPK signaling pathway and Wnt signaling pathway in L. vannamei. These results improved our current understanding of the L. vannamei UPR, and highlighted its importance in cell homeostasis upon environmental stress. PMID:26497095

  17. Black gill disease of Paciifc white leg shrimp (Litopenaeus vannamei) byAspergillus lfavus

    Institute of Scientific and Technical Information of China (English)

    Naresh Kumar Dewangan; Ayyaru Gopalakrishnan; Daniel Kannan; Narayanasamy Shettu; Ramakrishna Rajkumar Singh

    2015-01-01

    Objective:To study the epidemiology of black gill disease in white leg shrimp which is a major problem being faced by the commercial shrimp farmers who are culturingLitopenaeus vannamei (L. vannamei) in India. Methods:The normal and infected shrimps were collected from shrimp pond and the gill was preserved in appropriate preservative for histopathological examination and scanning electron microscope analysis. Pathogenic fungus was isolated from black gill of L. vannameiin potato dextrose agar medium. Morphological study and fungal strain identification were done by using light microscopy and scanning electron microscope. FungalDNA was amplified byITS4 andITS5 primers and gene sequencing was done by Macrogen Inc., Korea. Phylogenetic tree was prepared by usingMEGA 6 software. Results:Fungal spores and hyphae were observed both in internal and external gill surface of infected shrimps. Fungal spores were round in shape and mature sporangium was observed. The histopathology study showed clearly that infected gill was damaged by the fungi. Scanning electron microscopic study showed adherence of fungi in infected gill. Internal transcribed spacer gene sequencing revealed that it was caused by Aspergillus flavus. Conclusions: The outcome of the present study would help to know the cause of black gill disease and to understand theeffectof pathogenic fungi in shrimp culture. This study will initiate researchers for work in field of treatment or prevention of black gill disease in commercial L. vannameiculture.

  18. Black gill disease of Pacific white leg shrimp (Litopenaeus vannamei by Aspergillus flavus

    Directory of Open Access Journals (Sweden)

    Naresh Kumar Dewangan

    2015-10-01

    Full Text Available Objective: To study the epidemiology of black gill disease in white leg shrimp which is a major problem being faced by the commercial shrimp farmers who are culturing Litopenaeus vannamei (L. vannamei in India. Methods: The normal and infected shrimps were collected from shrimp pond and the gill was preserved in appropriate preservative for histopathological examination and scanning electron microscope analysis. Pathogenic fungus was isolated from black gill of L. vannamei in potato dextrose agar medium. Morphological study and fungal strain identification were done by using light microscopy and scanning electron microscope. Fungal DNA was amplified by ITS4 and ITS5 primers and gene sequencing was done by Macrogen Inc., Korea. Phylogenetic tree was prepared by using MEGA 6 software. Results: Fungal spores and hyphae were observed both in internal and external gill surface of infected shrimps. Fungal spores were round in shape and mature sporangium was observed. The histopathology study showed clearly that infected gill was damaged by the fungi. Scanning electron microscopic study showed adherence of fungi in infected gill. Internal transcribed spacer gene sequencing revealed that it was caused by Aspergillus flavus. Conclusions: The outcome of the present study would help to know the cause of black gill disease and to understand the effect of pathogenic fungi in shrimp culture. This study will initiate researchers for work in field of treatment or prevention of black gill disease in commercial L. vannamei culture.

  19. The Effect of Three Culture Methods on Intensive Culture System of Pacific White Shrimp (Litopenaeus vannamei)

    Institute of Scientific and Technical Information of China (English)

    MA Zhen; WAN Rong; SONG Xiefa; GAO Lei

    2013-01-01

    Different culture methods may affect the intensive culture system of Pacific white shrimp (Litopenaeus vannamei) regarding water quality and growth and economic performance.This study evaluated the potential effects of three culture methods through cultivation of juvenile shrimps under consistent tank management conditions for 84d.The three methods involved shrimp cultivation in different tanks,i.e.,outdoor tanks with cement bottom (mode-C),greenhouse tanks with cement bottom (mode-G) and outdoor tanks with mud-substrate (mode-M).Results showed that water temperature was significantly higher in mode-G than that in mode-C (P <0.05).In contrast to the other two treatments,mode-M had stable pH after 50d cultivation of shrimps.In the mid-late period,the average concentrations of TAN,NO2-N,DIP and COD were significantly lower in mode-M and mode-G compared with those in mode-C (P <0.05).Despite lack of differences in the final shrimp weight among different treatments (P >0.05),mode-M had significantly higher shrimp yield,survival rate and feed conversion rate (P < 0.05) than other modes.There were significant differences in revenue and net return among different treatments (P<0.05).These demonstrated that the treatments of mode-G and mode-M were conductive to the intensive culture system ofL.vannamei.

  20. Antimutagenic Compounds of White Shrimp (Litopenaeus vannamei: Isolation and Structural Elucidation

    Directory of Open Access Journals (Sweden)

    Carmen-María López-Saiz

    2016-01-01

    Full Text Available According to the World Health Organization, cancer is the main cause of mortality worldwide; thus, the search of chemopreventive compounds to prevent the disease has become a priority. White shrimp (Litopenaeus vannamei has been reported as a source of compounds with chemopreventive activities. In this study, shrimp lipids were extracted and then fractionated in order to isolate those compounds responsible for the antimutagenic activity. The antimutagenic activity was assessed by the inhibition of the mutagenic effect of aflatoxin B1 on TA98 and TA100 Salmonella tester strains using the Ames test. Methanolic fraction was responsible for the highest antimutagenic activity (95.6 and 95.9% for TA98 and TA100, resp. and was further separated into fifteen different subfractions (M1–M15. Fraction M8 exerted the highest inhibition of AFB1 mutation (96.5 and 101.6% for TA98 and TA100, resp. and, after further fractionation, four subfractions M8a, M8b, M8c, and M8d were obtained. Data from 1H and 13C NMR, and mass spectrometry analysis of fraction M8a (the one with the highest antimutagenic activity, suggest that the compound responsible for its antimutagenicity is an apocarotenoid.

  1. The cytosolic manganese superoxide dismutase from the shrimp Litopenaeus vannamei: molecular cloning and expression.

    Science.gov (United States)

    Gómez-Anduro, Gracia A; Barillas-Mury, Carolina-V; Peregrino-Uriarte, Alma B; Gupta, Lalita; Gollas-Galván, Teresa; Hernández-López, Jorge; Yepiz-Plascencia, Gloria

    2006-01-01

    Manganese containing superoxide dismutase (SOD) is normally a nuclear-encoded mitochondrial enzyme in eukaryotic organisms; however, a cytoplasmic manganese SOD (cMnSOD) was found in crustaceans that use hemocyanin as oxygen carrier. The complete cDNA and deduced amino acid sequence of a cMnSOD from Litopenaeus vannamei were determined. The coding sequence predicts a 287 residues protein with a unique 61 amino acids extension at the N-terminus and lacking a mitochondrial-targeting sequence. Phylogenetic analysis clusters cMnSODs and mitochondrial MnSODs in two separate groups. cMnSOD transcripts were detected in hemocytes, heart, hepatopancreas, intestine, nervous system, muscle, pleopods and gills. Since hemocytes are key defense cells and their reactions produce superoxide radicals, the infection by white spot syndrome virus on the cMnSOD transcript levels were investigated and found to increase transiently 1h post-infection and then decrease as the viral infection progressed to levels significantly lower than uninfected controls by 12h post-infection. PMID:16504292

  2. Catalase from the white shrimp Penaeus (Litopenaeus) vannamei: molecular cloning and protein detection.

    Science.gov (United States)

    Tavares-Sánchez, Olga L; Gómez-Anduro, Gracia A; Felipe-Ortega, Ximena; Islas-Osuna, Maria A; Sotelo-Mundo, Rogerio R; Barillas-Mury, Carolina; Yepiz-Plascencia, Gloria

    2004-08-01

    Catalase is an antioxidant enzyme that plays a very important role in the protection against oxidative damage by breaking down hydrogen peroxide. It is a very highly conserved enzyme that has been identified from numerous species including bacteria, fungi, plants and animals, but the information about catalase in crustaceans is very limited. A cDNA containing the complete coding sequence for catalase from the shrimp Penaeus (Litopenaeus) vannamei was sequenced and the mRNA was detected by RT-PCR in selected tissues. Catalase was detected in hepatopancreas crude extracts by Western blot analysis with anti-human catalase polyclonal antibodies. The nucleotide sequence is 1692 bp long, including a 72-bp 5'-UTR, a coding sequence of 1515 bp and a 104-bp 3'-UTR. The deduced amino acid sequence corresponds to 505 amino acids with high identity to invertebrate, vertebrate and even bacterial catalases and contains the catalytic residues His71, Asn144, and Tyr354. The predicted protein has a calculated molecular mass of 57 kDa; which coincides with the size of the subunit (approximately 55 kDa) and the tetrameric protein (approximately 230 kDa) detected in hepatopancreas extracts under native conditions. Catalase mRNA level was higher in hepatopancreas, followed by gills and was not detected in muscle. PMID:15325332

  3. Stylicins, a new family of antimicrobial peptides from the Pacific blue shrimp Litopenaeus stylirostris.

    Science.gov (United States)

    Rolland, J L; Abdelouahab, M; Dupont, J; Lefevre, F; Bachère, E; Romestand, B

    2010-03-01

    The present study reports the characterization of Ls-Stylicin1, a novel antimicrobial peptide from the penaeid shrimp, Litopenaeus stylirostris. The predicted mature peptide of 82 residues is negatively charged (theoretical pI=5.0) and characterized by a proline-rich N-terminal region and a C-terminal region containing 13 cysteine residues. The recombinant Ls-Stylicin1 has been isolated in both monomeric and dimeric forms. Both display strong antifungal activity against Fusarium oxysporum (1.25 microM

  4. Molecular phylogeny of western Atlantic Farfantepenaeus and Litopenaeus shrimp based on mitochondrial 16S partial sequences.

    Science.gov (United States)

    Maggioni, R; Rogers, A D; Maclean, N; D'Incao, F

    2001-01-01

    Partial sequences for the 16S rRNA mitochondrial gene were obtained from 10 penaeid shrimp species: Farfantepenaeus paulensis, F. brasiliensis, F. subtilis, F. duorarum, F. aztecus, Litopenaeus schmitti, L. setiferus, and Xiphopenaeus kroyeri from the western Atlantic and L. vannamei and L. stylirostris from the eastern Pacific. Sequences were also obtained from an undescribed morphotype of pink shrimp (morphotype II) usually identified as F. subtilis. The phylogeny resulting from the 16S partial sequences showed that these species form two well-supported monophyletic clades consistent with the two genera proposed in a recent systematic review of the suborder Dendrobranchiata. This contrasted with conclusions drawn from recent molecular phylogenetic work on penaeid shrimps based on partial sequences of the mitochondrial COI region that failed to support recent revisions of the Dendrobranchiata based on morphological analysis. Consistent differences observed in the sequences for morphotype II, coupled with previous allozyme data, support the conclusion that this is a previously undescribed species of Farfantepenaeus. PMID:11161743

  5. Nitrite toxicity of Litopenaeus vannamei in water containing low concentrations of sea salt or mixed salts

    Science.gov (United States)

    Sowers, A.; Young, S.P.; Isely, J.J.; Browdy, C.L.; Tomasso, J.R., Jr.

    2004-01-01

    The uptake, depuration and toxicity of environmental nitrite was characterized in Litopenaeus vannamei exposed in water containing low concentrations of artificial sea salt or mixed salts. In 2 g/L artificial sea salts, nitrite was concentrated in the hemolymph in a dose-dependent and rapid manner (steady-state in about 2 d). When exposed to nitrite in 2 g/L artificial sea salts for 4 d and then moved to a similar environment without added nitrite, complete depuration occurred within a day. Increasing salinity up to 10 g/L decreased uptake of environmental nitrite. Nitrite uptake in environments containing 2 g/L mixed salts (combination of sodium, potassium, calcium and magnesium chlorides) was similar to or lower than rates in 2 g/L artificial sea salt. Toxicity was inversely related to total dissolved salt and chloride concentrations and was highest in 2 g/L artificial sea salt (96-h medial lethal concentration = 8.4 mg/L nitrite-N). Animals that molted during the experiments did not appear to be more susceptible to nitrite than animals that did not molt. The shallow slope of the curve describing the relationship between toxicity and salinity suggests that management of nitrite toxicity in low-salinity shrimp ponds by addition of more salts may not be practical. ?? Copyright by the World Aquaculture Society 2004.

  6. A Comparative Study on the Nonspecific Immunity of Juvenile Litopenaeus vannamei ever Inhabiting Freshwater and Seawater

    Institute of Scientific and Technical Information of China (English)

    JIA Xuying; DING Sen; WANG Fang; DONG Shuanglin

    2014-01-01

    A study on the nonspecific immunity of Litopenaeus vannamei ever inhabiting freshwater and seawater was carried out at different molt stages by comparing their total hemocyte count (THC) and respiratory burst (RB) and activity of phenol oxidase (PO), nitric oxide synthase (NOS) and lysozyme (LY). Two-way ANOVA showed that salinity and molt stage independently affected THC and RB and the activity of PO, NOS and LY of juvenile L. vannamei significantly (P<0.05). The THC and RB and the activity of NOS gradually increased from the post-molt stages (A and B) to the pre-molt stages (D0-D3), which were common in shrimps inhabiting freshwater and seawater. The activity of PO peaked at the inter-molt stage (C), and touched the lowest at the post-molt stage in freshwater and pre-molt stage in seawater. The activity of LY was stable over the molt cycle. The RB and the activity of PO, NOS and LY of juvenile L. vannamei were significantly lower in freshwater than in seawater;whereas THC was significantly higher in freshwater than in seawater (P<0.05). It was concluded that the post-molt stage (especially stage A) was critical to shrimp culture, which should be intensively attended when L. vannamei was cultured in freshwater.

  7. Mercury content and their risk assessment in farmed shrimp Litopenaeus vannamei from NW Mexico.

    Science.gov (United States)

    Delgado-Alvarez, C G; Ruelas-Inzunza, J; Osuna-López, J I; Voltolina, D; Frías-Espericueta, M G

    2015-01-01

    The main objective of this study was to evaluate the total mercury content in hepatopancreas and edible muscle of the whiteleg shrimp Litopenaeus vannamei cultured along the NW coast of Mexico, and to evaluate the potential human health risk due to their consumption. Samples were obtained between May and June 2010 in 26 shrimp farms from the three most important shrimp-producing states of NW Mexico, and total Hg was analyzed after reduction with SnCl2 in a mercury analyzer. The ranges of Hg concentrations of the hepatopancreas were 0.101±0.03-0.184±0.13 μg g(-1) in Sonora, 0.077±0.055-0.813±0.363 μg g(-1) in Sinaloa and 0.139±0.037-0.791±0.33 μg g(-1) in Nayarit. In the muscle, values were from 0.078±0.02 to 0.539±0.09 μg g(-1) in Sonora, 0.154±0.03-0.861±0.423 μg g(-1) in Sinaloa and 0.121±0.041-1.48±0.44 μg g(-1) in Nayarit. Considering the concentrations of Hg in the muscle and the national consumption rate, shrimp farmed in NW Mexico does not represent a risk for human health (HQ<1).

  8. A Comparative study on the nonspecific immunity of juvenile Litopenaeus vannamei ever inhabiting freshwater and seawater

    Science.gov (United States)

    Jia, Xuying; Ding, Sen; Wang, Fang; Dong, Shuanglin

    2014-06-01

    A study on the nonspecific immunity of Litopenaeus vannamei ever inhabiting freshwater and seawater was carried out at different molt stages by comparing their total hemocyte count (THC) and respiratory burst (RB) and activity of phenol oxidase (PO), nitric oxide synthase (NOS) and lysozyme (LY). Two-way ANOVA showed that salinity and molt stage independently affected THC and RB and the activity of PO, NOS and LY of juvenile L. vannamei significantly ( P < 0.05). The THC and RB and the activity of NOS gradually increased from the post-molt stages (A and B) to the pre-molt stages (D0-D3), which were common in shrimps inhabiting freshwater and seawater. The activity of PO peaked at the inter-molt stage (C), and touched the lowest at the post-molt stage in freshwater and pre-molt stage in seawater. The activity of LY was stable over the molt cycle. The RB and the activity of PO, NOS and LY of juvenile L. vannamei were significantly lower in freshwater than in seawater; whereas THC was significantly higher in freshwater than in seawater ( P < 0.05). It was concluded that the post-molt stage (especially stage A) was critical to shrimp culture, which should be intensively attended when L. vannamei was cultured in freshwater.

  9. A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance

    Science.gov (United States)

    Liu, Jingwen; Yu, Yang; Li, Fuhua; Zhang, Xiaojun; Xiang, Jianhai

    2014-11-01

    Anti-lipopolysaccharide factors (ALFs) are basic components of the crustacean immune system that defend against a range of pathogens. The cDNA sequence of a new ALF, designated nLvALF2, with an open reading frame encoding 132 amino acids was cloned. Its deduced amino acid sequence contained the conserved functional domain of ALFs, the LPS binding domain (LBD). Its genomic sequence consisted of three exons and four introns. nLvALF2 was mainly expressed in the Oka organ and gills of shrimps. The transcriptional level of nLvALF2 increased significantly after white spot syndrome virus (WSSV) infection, suggesting its important roles in protecting shrimps from WSSV. Single nucleotide polymorphisms (SNPs) were found in the genomic sequence of nLvALF2, of which 38 were analyzed for associations with the susceptibility/resistance of shrimps to WSSV. The loci g.2422 A>G, g.2466 T>C, and g.2529 G>A were significantly associated with the resistance to WSSV ( P<0.05). These SNP loci could be developed as markers for selection of WSSV-resistant varieties of Litopenaeus vannamei.

  10. Antibiotic resistance of Vibrio parahaemolyticus isolated from pond-reared Litopenaeus vannamei marketed in Natal, Brazil

    Directory of Open Access Journals (Sweden)

    Ligia Maria Rodrigues de Melo

    2011-12-01

    Full Text Available Ten out of fifty fresh and refrigerated samples of shrimp (Litopenaeus vannamei collected from retailers in Natal (Rio Grande do Norte, Northeastern Brazil tested positive for Vibrio parahaemolyticus. The Kanagawa test and multiplex PCR assays were used to detect TDH and TRH hemolysins and the tdh, trh and tlh genes, respectively. All strains were Kanagawa-negative and tlh-positive. Antibiotic susceptibility testing was done for seven antibiotics by the agar diffusion technique. Five strains (50% presented multiple antibiotic resistance to ampicillin (90% and amikacin (60%, while two strains (20% displayed intermediate-level resistance to amikacin. All strains were sensitive to chloramphenicol. Intermediate-level susceptibility and/or resistance to other antibiotics ranged from 10 to 90%, with emphasis on the observed growing intermediate-level resistance to ciprofloxacin. Half our isolates yielded a multiple antibiotic resistance index above 0.2 (range: 0.14-0.29, indicating a considerable risk of propagation of antibiotic resistance throughout the food chain.

  11. Viabilidad fitoclimática de las repoblaciones de pino silvestre (Pinus sylvestris L. en la Sierra de los Filabres (Almería

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    A. Fernández Cancio

    2011-01-01

    Full Text Available La variabilidad climática actual se caracteriza por provocar cambios bruscos en los cursos fitoclimáticos, lo que está desencadenando procesos de mortalidad de especies forestales. El objetivo de este trabajo es analizar la adecuación fitoclimática de las masas artificiales de Pinus sylvestris L. en la Sierra de los Filabres (Almería, para lo cual se analizaron 35 variables fitoclimáticas que determinan la estabilidad climática de la especie. El estudio de los valores interanuales de las variables climáticas indica que entre 1970 y 1980 comenzó una perturbación climática amplia, modificando la estacionalidad y la variabilidad de las precipitaciones y de las temperaturas. Como rasgos más significativos tenemos el aumento de las temperaturas, sobre todo de las máximas, el aumento de la oscilación térmica y de los extremos diarios, la disminución global de precipitación, el aumento grave de la aridez primaveral y el deterioro de todas las variables ómbricas. El estudio de las estaciones con masas repobladas de pino silvestre en Andalucía oriental indica que las estaciones están fuera de la nube de puntos de las masas de pino silvestre establecidas en España. Las masas de pino silvestre de la Sierra de los Filabres están notablemente fuera de estación en todas sus características fitoclimáticas y pueden desaparecer progresiva o bruscamente, sobre todo si se implanta un cambio climático.

  12. ANÁLISIS DEL CRECIMIENTO DE UN GENOTIPO SILVESTRE DE Carica papaya L. CULTIVADO EX SITU Y CV. `MARADOL ROJA ́´

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    Jesús Rodríguez Cabello

    2015-01-01

    Full Text Available La especie Carica papaya L., es nativa del trópico americano y uno de los frutales más cultivados a nivel mundial. En Cuba existen problemas que afectan la producción de este frutal, considerados los más importantes el reducido número de cultivares explotados comercialmente y la baja diversidad genética presente en el banco de germoplasma. Sin embargo, existen genotipos silvestres que constituyen una fuente importante de genes para los programas de mejoramiento y explotación local, los cuales aún no se han caracterizado. Estudios de análisis del crecimiento permiten comprender su comportamiento en diferentes periodos de siembra en condiciones ex situ eidentificar caracteres de crecimiento inicial que posibiliten el aumento del rendimiento y favorezcan los trabajos de mejoramiento en busca de genotipos más productivos. Debido a lo antes señalado, el objetivo de la presente investigación fue realizar estudios del crecimiento del genotipo papaya silvestre de la cuenca Almendares-Vento. Las semillas para los experimentos se obtuvieron de frutos colectados in situ. Las medidas directas en la planta fueron área foliar y peso seco. Para la determinación de la superficie foliar se empleó una cámara digital y un software. El análisis computarizado de las imágenes de hojas obtenidas con la cámara digital permitió estimar el área foliar de forma simple, rápida y económica. La mayor producción de área foliar del genotipo silvestre y similar comportamiento en la masa seca y resto de los indicadores evaluados, indica la disponibilidad de recursos durante toda la fase de crecimiento, lo cual se asocia con su rendimiento

  13. Comparative studies of hemolymph physiology response and HIF-1 expression in different strains of Litopenaeus vannamei under acute hypoxia.

    Science.gov (United States)

    Wei, Lin; Li, Yuhu; Qiu, Liguo; Zhou, Hailong; Han, Qian; Diao, Xiaoping

    2016-06-01

    Litopenaeus vannamei has a high commercial value and is the primary cultured shellfish species globally. In this study, we have compared the hemolymph physiological responses between two L. vannamei strains under acute hypoxia. The results showed that hemocyanin concentration (HC) of strain A6410 was significantly higher than strain Zhengda; Total hemocyte counts (THC) decreased significantly in both strains under hypoxic stress (p  0.05), but in the gills and hepatopancreas under hypoxia for 12 h (p selective breeding process of hypoxia tolerance in L. vannamei. PMID:27016815

  14. Physicochemical, Proximate Composition, Microbiological and Sensory Analysis of Farmed and Wild Harvested White Shrimp Litopenaeus vannamei (Boone, 1931 Tissues

    Directory of Open Access Journals (Sweden)

    M. García-ulloa Gomez

    2013-05-01

    Full Text Available Physicochemical, proximate composition, microbiological and sensory analysis of farmed and wild harvested white shrimp Litopenaeus vannamei muscle were compared. The cultured white shrimp were obtained of two farms, whereas the wild shrimp were collected off the coasts of Sinaloa and Nayarit, Mexico. Both, the farmed and wild white shrimp muscle, supplied a good source of protein and polyunsaturated fatty acids. The physicochemical composition, microbiological and sensory properties could be associated to their origin and handling. The wild shrimp tended to have a better proximate composition than the farmed shrimp, due to the availability of a greater diet variety in their environment.

  15. Effect of dietary probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress status of shrimp Litopenaeus stylirostris

    OpenAIRE

    Castex, Mathieu; Lemaire, Pierrette; Wabete, Nelly; Chim, Liet

    2009-01-01

    in this study we evaluated the effects of the probiotic Pediococcus acidilactici MA18/5M on shrimp, Litopenaeus stylirostris (also called Penaeus stylirostris), first on antioxidant defences and secondly on the oxidative stress status in the shrimps' haemolymph and digestive gland. We conducted two experiments with the same protocol in which shrimps were fed two diets for three weeks: a control diet and a probiotic diet containing 1 g of live P. acidilactici MA18/5M kg(-1). In the first exper...

  16. Response of the blue shrimp Litopenaeus stylirostris to temperature decrease and hypoxia in relation to molt stage

    OpenAIRE

    Mugnier, Chantal; Soyez, Claude

    2005-01-01

    The effect of different levels of dissolved oxygen (DO) and a decreased temperature combined with severe hypoxia were studied in the blue shrimp Litopenaeus stylirostris in relation to molt stage. In the first experiment, shrimps were submitted to DO concentrations of 5 to 1 mg l(-1). Osmoregulatory capacity (OC) was measured in shrimps at molt stage C after 6, 24, or 48 h of exposure. No mortality was recorded but a significant negative effect of DO concentration on hypo-OC was observed for ...

  17. Quality changes during frozen storage of blue shrimp (Litopenaeus stylirostris) with antioxidant, α-tocopherol, under different conditions

    OpenAIRE

    Adriana Zulema Valencia-Perez; Herlinda Soto-Valdez; Josafat Marina Ezquerra-Brauer; Enrique Márquez-Ríos; Wilfrido Torres-Arreola

    2015-01-01

    Fresh blue shrimp (Litopenaeus stylirostris) muscle was stored with antioxidants under different conditions: ANTIOX 2%, packed in bilayer film of polyamide-low density polyethylene film (PA-LDPE) with 2% α-tocopherol; ANTIOX 4%, packed in PA-LDPE film with 4% α-tocopherol; and ANTIOX-GLAZED, samples stored glazed with 2% α-tocopherol. Shrimps packed in PA-LDPE without α-tocopherol were used as CONTROL. All samples were stored at –20 °C for 120 days. As compared to the CONTROL, the shrimp stor...

  18. Nitrogen dynamics model in zero water exchange, low salinity intensive ponds of white shrimp, Litopenaeus vannamei, at Colima, Mexico

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    Francisco A Castillo-Soriano

    2013-03-01

    Full Text Available We present a mathematical model based on differential equations describing the dynamics of nitrogen (NH4+, NO2-, NO3- and organic nitrogen in phytoplankton in ponds of white shrimp (Litopenaeus vannamei, with low salinity and zero turnovers, from planting to harvest. The model predicts the results of commercial production in three ponds. We show that this culture system, without replacement, retains the nitrogen and shrimp produced a lower feed conversion in comparison with systems with replacement. The model can be used to define strategies for improved performance.

  19. Fauna silvestre asociada a ganado vacuno doble propósito en sistema de silvopastoreo, Pinto, Magdalena, Colombia

    Directory of Open Access Journals (Sweden)

    Jaime De La Ossa V.

    2011-12-01

    Full Text Available Objetivo. Evaluar la biodiversidad faunística asociada a ganadería vacuna de doble propósito en dos potreros estructurados con Botriochloa pertusa (colosoana como herbácea dominante, uno de ellos plantado con Crescentia cujete (Bignoniaceae como fuente de alimento adicional y otro con escaso y disperso arbolado. Materiales y métodos. El área de estudio está ubicada en el municipio de Pinto, Magdalena. Cada potrero evaluado fue de 10 ha, se mantuvo en cada uno un lote de quince vacas (½ Cebú, ¼ Pardo y ¼ Costeño con Cuernos, la separación entre las áreas muestreadas fue de 500m. Los registros de fauna se condujeron mediante punto fijo de conteo, en diez sitios de avistamiento, durante 20 días continuos, con cubrimiento visual amplio. Al ganado vacuno adulto presente en las dos áreas de trabajo se le realizó un pesaje individual al inicio del trabajo y al final del mismo, con el fin de determinar posibles diferencias entre ellos. Resultados. Estadísticamente el número de individuos y el número de especies presentaron diferencias significativas, siendo mayor la diversidad biológica para el potrero que poseía la plantación de C. cujete, mientras que el peso corporal de las vacas sometidas a pastoreo en las dos áreas no presentó diferencias significativas. Conclusiones. Se demuestra la importancia de este modelo pecuario como promotor de la conservación faunística local al convertirse enrefugio de variadas especies silvestres, al tiempo que mantiene la productividad existente sin alterar el ambiente sustancialmente.

  20. Revisión del concepto biológico desarrollado en la legislación colombiana relacionada con la fauna silvestre vertebrada terrestre

    Directory of Open Access Journals (Sweden)

    Maldonado Chaparro Adriana A.

    2004-12-01

    Full Text Available En el trabajo se presenta un panorama general del estado actual de la legislación en materia de fauna silvestre vertebrada terrestre en Colombia, el cual es complementado con algunas actividades de conservación y aprovechamiento del recurso faunístico, que han sido adelantadas por
    las autoridades ambientales. Además, se exponen y analizan conceptos biológicos usados dentro de la normatividad, que son considerados claves desde el punto de vista biológico para la aplicación de las normas.

  1. Diagnóstico del tráfico ilegal y del manejo post decomiso de fauna silvestre en nueve corporaciones autónomas regionales de Colombia

    Directory of Open Access Journals (Sweden)

    Montenefgro Olga Lucía

    2007-12-01

    Full Text Available La fauna silvestre siempre ha estado relacionada con el hombre sirviéndole de diferentes maneras, ya sea como fuente de alimento, como compañía, como materia prima para la fabricación de accesorios (pieles y otros productos o como diversión. Una de las causas más importantes de pérdida de diversidad la constituye el aprovechamiento no sostenible de especies de fauna y flora tanto para el consumo directo como para el comercio, lo que conlleva a la reducción en el tamaño de las poblaciones silvestres y por tanto una mayor vulnerabilidad a la extinción. El tráfico ilegal de especies silvestres es considerado a nivel mundial como una de las principales causas de disminución de las poblaciones naturales y como uno de los mercados ilegales que mayores sumas de dinero moviliza anualmente, con el fin de surtir la demanda de los mercados mundiales de la moda, las excentricidades de pequeños grupos, las investigaciones biomédicas y comportamentales, la novedad causada a turistas y las prácticas culturales o religiosas de muchas poblaciones humanas. En Colombia uno de los problemas de conservación más complejos lo representa el manejo de aquellos especímenes de fauna silvestre que son objeto de decomiso por parte de las autoridades ambientales regionales. Para lograr una adecuada disposición de la gran cantidad de animales silvestres confiscados o entregados voluntariamente en Colombia, el Ministerio del Medio Ambiente y las demás autoridades ambientales acordaron poner en marcha nueve centros regionales para el manejo de fauna decomisada o
    Centros de Atención y Valoración (CAV. Esta disposición tenía el propósito de implementar parámetros y protocolos que faciliten tomar decisiones que tanto las instituciones como los profesionales encargados enfrentan a diario. Sin embargo, el manejo de la fauna postdecomiso continua siendo uno de los problemas más complejos debido a la gran cantidad de animales decomisados y a la poca

  2. Estudo de plantas silvestres comestíveis da região mediterrânica como fontes de compostos bioativos

    OpenAIRE

    Moreira, Elsa Cristina da Silva

    2015-01-01

    Dissertação de mestrado, Engenharia Biológica, Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2015 Atualmente as plantas silvestres comestíveis são muito procuradas com o intuito de introduzir novos sabores e texturas na alimentação, particularmente na gastronomia gourmet. Os benefícios para a saúde, decorrentes dos elevados níveis de compostos bioativos presentes nestas plantas, são um adicional motivo de interesse. O objetivo deste trabalho foi avaliar o potencial de extrat...

  3. LA DOMESTICACIÓN DE PLANTAS EN MÉXICO: COMPARACIÓN DE LA FORMA CULTIVADA Y SILVESTRE DE BYRSONIMA CRASSIFOLIA (MALPIGHIACEAE)

    OpenAIRE

    J.C. Raya-Pérez; C.L. Aguirre-Mancilla; K. Gil-Vega; Simpson, J

    2010-01-01

    Las plantas domesticadas presentan una serie de características conocidas como síndrome de domesticación. Esto permite un mejor aprovechamiento de las mismas por parte de los humanos y ha sido estudiada principalmente en plantas anuales herbáceas. En este trabajo se hizo un muestreo de árboles silvestres (changunga) y cultivados (nanche) de Byrsonima crassifolia en la región de Uruapan, México. El objetivo fue buscar diferencias genéticas mediante marcadores moleculares por medio de la técnic...

  4. CARACTERÍSTICAS MORFOLÓGICAS DE FORMAS CULTIVADAS, SILVESTRES E INTERMEDIAS DE FRIJOL COMÚN DE HÁBITO TREPADOR

    OpenAIRE

    Rogelio Lépiz Ildefonso; J. Jesús López Alcocer; J. Jesús Sánchez González; Fernando Santacruz-Ruvalcaba; Ricardo Nuño Romero; Eduardo Rodríguez Guzmán

    2010-01-01

    Se hizo una caracterización morfológica de formas cultivadas, silvestres e intermedias de frijol común (Phaseolus vulgaris L., Fabaceae) de hábito trepador. Se evaluaron 15 materiales en un diseño de bloques completos al azar con tres repeticiones. Para determinar las relaciones morfológicas entre las formas incluidas, se registraron 18 variables cuantitativas con lo que se hicieron análisis de varianza, de agrupamiento con base en la distancia euclidiana y componentes principales. Doce de lo...

  5. Diagnóstico del tráfico ilegal y del manejo post decomiso de fauna silvestre en nueve corporaciones autónomas regionales de Colombia

    OpenAIRE

    Montenefgro Olga Lucía; Mendivelso Gamboa Diego Andrés

    2007-01-01

    La fauna silvestre siempre ha estado relacionada con el hombre sirviéndole de diferentes maneras, ya sea como fuente de alimento, como compañía, como materia prima para la fabricación de accesorios (pieles y otros productos) o como diversión. Una de las causas más importantes de pérdida de diversidad la constituye el aprovechamiento no sostenible de especies de fauna y flora tanto para el consumo directo como para el comercio, lo que conlleva a la reducción en el tamaño de las poblaciones sil...

  6. Efecto de la salinidad en la sobrevivencia de peces silvestres del género Chirostoma durante el transporte y mantenimiento en laboratorio

    OpenAIRE

    Guillermo Artemio Blancas-Arroyo; Rocío Frías-Sevilla; Eréndira De la Rosa-Pimentel; Virginia Suárez-Navarro; José Rodolfo Castro-Gómez; Javier Magaña-Morales

    2014-01-01

    Se comprobó el efecto de la salinidad en la sobrevivencia de peces silvestres del género Chirostoma, durante su transporte y aclimatación. Estos organismos son endémicos de México y conocidos como peces blancos y charales; los cuales se han caracterizado por su hipersensibilidad al manejo y su alto grado de estrés. Los modelos biológicos fueron dos especies de charales: Chirostoma jordani de Xochimilco cuenca de México y C. chapalae de Queréndaro subcuenca de Cuitzeo Michoacán. Se transportar...

  7. Acumulación de cromo en setas silvestres comestibles: factores que influyen en su captación y repercusiones toxicológicas

    OpenAIRE

    MA García; Alonso, J.; AJ Martín; MJ Melgar

    2008-01-01

    La polución metálica medioambiental ha incrementado notablemente el interés por los hongos por ser captadores de metales pesados. Nutricionalmente, el cromo puede considerarse incluso un oligoelemento, pero en dosis excesivas es nocivo para la salud. Los objetivos planteados son la determinación del contenido de cromo en setas comestibles silvestres, el análisis estadístico de factores que influyen en su captación, y finalmente la evaluación del riesgo toxicológico deriva...

  8. Opciones de gestión para reducir la cacería ilegal: El caso de la Reserva de Vida Silvestre Amazónica Manuripi, Bolivia

    Directory of Open Access Journals (Sweden)

    S. Espinoza

    2013-01-01

    Full Text Available La cacería ilegal representa una amenaza para la conservación de la vida silvestre. Las motivaciones y efectos que puede tener son variables y aún no han sido ampliamente estudiados. Aplicando un enfoque teórico-conceptual económico analizamos los diferentes factores que pueden motivar la cacería ilegal y discutimos las implicaciones de gestión. Basándonos en el caso de la cacería ilegal que ocurre durante la recolección de castaña (Bertholletia excelsa en la Reserva de Vida Silvestre Amazónica Manuripi, Bolivia, analizamos los resultados de un extensivo estudio de incumpli- miento. En base a la revisión de fuentes primarias y secundarias de información y al marco conceptual referido a modelos económicos de disuasión, identificamos que la probabilidad de detección y sanción, la moralidad, la legitimidad y la influencia social pueden afectar el cumplimiento de la pro- hibición de caza en la Reserva Manuripi. Adicionalmente, consideramos que el conocimiento de la regulación también puede afectar la decisión de cazar y señalamos evidencia empírica que sugiere que el hábito de consumir carne silvestre y la no-disponibilidad de alternativas a la carne silvestre motivan la cacería ilegal en Manuripi. Bajo este análisis, las recomendaciones para disminuir el riesgo de cacería ilegal durante el periodo de reco- lección de castaña se enfocan en mejorar los esfuerzos de fiscalización, monitoreo y control por parte de la Autoridad Ambiental, paralelamente a la implementación de procesos de concientización dirigidos a modificar los valores éticos y hábitos culturales entre los recolectores.

  9. Intoxicación por munición de plomo en aves silvestres en España y medidas para reducir el riesgo

    OpenAIRE

    Mateo Soria, Rafael; Vallverdú Coll, Núria; Ortiz Santaliestra, Manuel Eloy

    2013-01-01

    La intoxicación por ingestión de munición de plomo en aves silvestres es conocida en España desde finales del siglo pasado. Elevadas densidades de perdigones de plomo en diversos humedales de importancia internacional producen tasas de ingestión de perdigones cercanas al 70% en especies como el ánade rabudo (Anas acuta) y el plumbismo llega a ser una de las principales causas de mortalidad de la amenazada malvasía cabeciblanca (Oxyura leucocephala). El plumbismo también ha sido descrito en di...

  10. Hongos comestibles silvestres: especies exóticas de Suillus (Boletales, Basidiomycota y Lactarius (Russulales, Basidiomycota asociados a cultivos de Pinus elliottii del nordeste argentino

    Directory of Open Access Journals (Sweden)

    Nicolás Niveiro

    2009-01-01

    Full Text Available Los hongos silvestres comestibles son un recurso económico importante que permite obtenerun producto deshidratado que puede comercializarse fácilmente. En el presente trabajo, y conel objeto de dar a conocer este recurso, se ilustran y describen dos especies de hongosmicorrícicos asociadas a cultivos de Pinus elliottii en el nordeste argentino que no han sidomencionadas anteriormente para la región: Lactarius deliciosus para la provincia de Misionesy Suillus granulatus para las provincias de Corrientes y Misiones

  11. Detección de Rickettsia spp. en Suero y Garrapatas de Mamíferos Silvestres en Cautiverio en Montería, Córdoba -resumen-

    OpenAIRE

    Dave Wehdeking-Hernández; Laura Hernández-Fernández; César Rojano-Bolaño; J Ruíz-Rodríguez

    2014-01-01

    Las rickettsiosis transmitidas por garrapatas son causadas por bacterias intracelulares pertenecientes al grupo de las fiebres manchadas del género Rickettsia. Estas zoonosis son algunas de las enfermedades transmitidas por vectores de más antiguo conocimiento‚ e incluyen dentro de su ciclo de transmisión diversas especies de mamíferos. No obstante‚ en Colombia existen pocos trabajos enfocados a conocer la dinámica de las rickettsiosis en animales silvestres. El objetivo de este estudio fue d...

  12. Nuevas cepas de duganella aisladas de la rizosfera de olivo silvestre y cultivado y su uso en la producción de violaceína

    OpenAIRE

    Landa, Blanca B.; Aranda Ocampo, Sergio; Montes Borrego, Miguel; Castillo, Pablo

    2010-01-01

    [ES] La presente invención se refiere a unas cepas bacterianas de Duganella spp. obtenidas de la rizosfera de olivos silvestres y cultivados. Preferentemente las cepas son CECT 7779, CECT 7780 y CECT 7781 y más preferentemente la cepa bacteriana es CECT 7780. Además, la presente invención se refiere a sus combinaciones con otros microorganismos y a las composiciones que comprenden los productos anteriores, así como a un procedimiento para la producción de violaceína y a la violaceína produ...

  13. Nuevas cepas de duganella aisladas de la rizosfera de olivo silvestre y cultivado y su uso en la producción de violaceína

    OpenAIRE

    Landa, Blanca B.; Aranda Ocampo, Sergio; Montes Borrego, Miguel; Castillo Castillo, Miguel

    2010-01-01

    La presente invención se refiere a unas cepas bacterianas de Duganella spp. obtenidas de la rizosfera de olivos silvestres y cultivados. Preferentemente las cepas son CECT 7779, CECT 7780 y CECT 7781 y más preferentemente la cepa bacteriana es CECT 7780. Además, la presente invención se refiere a sus combinaciones con otros microorganismos y a las composiciones que comprenden los productos anteriores, así como a un procedimiento para la producción de violaceína y a la violaceína producida par...

  14. Draft Genome Sequence of Bacillus licheniformis CG-B52, a Highly Virulent Bacterium of Pacific White Shrimp (Litopenaeus vannamei), Isolated from a Colombian Caribbean Aquaculture Outbreak.

    Science.gov (United States)

    Gálvez, Eric J C; Carrillo-Castro, Katerine; Zárate, Lina; Güiza, Linda; Pieper, Dietmar H; García-Bonilla, Erika; Salazar, Marcela; Junca, Howard

    2016-01-01

    Bacillus licheniformis strain CG-B52 was isolated as the etiological agent producing a self-limited outbreak of high mortalities in commercial Litopenaeus vannamei culture ponds on the Colombian Caribbean coast in 2005. Here, we report its draft genome and three novel extrachromosomal elements that it harbors. PMID:27174263

  15. Effects of dietary supplementation of a commercial prebiotic on survival, growth, immune responses, and gut microbiota of the Pacific white shrimp, Litopenaeus vannamei

    Science.gov (United States)

    A 35-day feeding trial was conducted to evaluate growth, bacterial populations of the gastrointestinal tract (GIT), and immune responses of Litopenaeus vannamei fed diets containing the commercial prebiotic PrevidaTM. Four diets were formulated to contain Previda at 0, 0.2, 0.5, 1.0, or 1.6% by wei...

  16. Erratum to: Response to selection, heritability and genetic correlations between body weight and body size in Pacific white shrimp, Litopenaeus vannamei

    Institute of Scientific and Technical Information of China (English)

    Farafidy ANDRIANTAHINA; LIU Xiaolin; HUANG Hao; XIANG Jianhai

    2012-01-01

    The original version of the title of this article unfortunately contained a mistake.The spelling of the [ZResponese]was incorrect.The title should read:Response to selection,heritability and genetic correlations between body weight and body size in Pacific white shrimp,Litopenaeus vannamei.The publishing companies apologize to the authors.

  17. Effects of dietary supplementation of a commercial prebiotic Previda on survival, growth, immune responses and gut microbiota of pacific white shrimp, Litopenaeus vannamei

    Science.gov (United States)

    A 35-day feeding trial was conducted to evaluate growth, bacterial populations of the gastrointestinal tract (GIT), and immune responses of Litopenaeus vannamei fed diets containing the commercial prebiotic PrevidaTM. Four diets were formulated to contain Previda at 0, 0.2, 0.5, 1.0, or 1.6% by we...

  18. Caracterización anatómica y conductividad hidráulica teórica de los elementos de vaso de variedades silvestres y cultivadas de nopal tunero (Opuntia)

    OpenAIRE

    Eulogio Pimienta- Barrios; Sofía Loza Cornejo; María Eugenia González Del Castillo-Aranda

    2003-01-01

    Se estudió la morfología y la conductancia hidráulica teórica de elementos de vaso del xilema en cladodios de especies y variedades cultivadas y silvestres de nopal ( Opuntia spp.) colectados en las regiones semiáridas de los estados de Jalisco y Zacatecas, con el fin de investigar si las diferencias en la resistencia a la sequía que se observan comúnmente en el campo entre especies silvestres y cultivadas de Opuntia pueden estar relacionadas con divergencias en la densidad, la anatomía de lo...

  19. FONTES E NÍVEIS DE VITAMINA C PARA O CAMARÃO Litopenaeus vannamei NA FASE DE RECRIA

    Directory of Open Access Journals (Sweden)

    Neyva Ribeiro Ferraz

    2012-09-01

    Full Text Available The inclusion of different sources and levels of vitamin C was evaluated on growth, survival, protein retention and health of Litopenaeus vannamei with initial weight of 5.90 ± 0.57 g. Five hundred and forty individuals were distributed in 27 cages in a completely randomized design in a 4x2 factorial scheme (four sources and two levels (n = 3. The sources used were ascorbic acid crystal, coated ascorbic acid, ascorbic acid monophosphate and ascorbic acid PEG (polyethylene glycol with two levels: 180 and 260 mg kg-1. No influence was observed (P>0.05 from the source, level of vitamin C nor interaction of these factors on growth, survival, retention of protein and parameters of health of the shrimp. Necrosis was observed in the abdomen of all animals. The use of vitamin C in sources and levels estimated for the shrimp Litopenaeus vannamei weighing over 5 g shows no improvement in growth, survival, protein retention and health of shrimp.

  20. Evaluation of coexposure to inorganic arsenic and titanium dioxide nanoparticles in the marine shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Cordeiro, Lucas; Müller, Larissa; Gelesky, Marcos A; Wasielesky, Wilson; Fattorini, Daniele; Regoli, Francesco; Monserrat, José Marìa; Ventura-Lima, Juliane

    2016-01-01

    The acute toxicity of titanium dioxide nanoparticles (nTiO2) that occur concomitantly in the aquatic environment with other contaminants such as arsenic (As) is little known in crustaceans. The objective of the present study is to evaluate whether coexposure to nTiO2 can influence the accumulation, metabolism, and oxidative stress parameters induced by arsenic exposure in the gills and hepatopancreas of the shrimp Litopenaeus vannamei. Organisms were exposed by dissolving chemicals in seawater (salinity = 30) at nominal concentrations of 10 μg/L nTiO2 or As(III), dosed alone and in combination. Results showed that there was not a significant accumulation of As in either tissue type, but the coexposure altered the pattern of the metabolism. In the hepatopancreas, no changes were observed in the biochemical response, while in the gills, an increase in the glutamate-cysteine-ligase (GCL) activity was observed upon exposure to As or nTiO2 alone, an increase in the reduced glutathione (GSH) levels was observed upon exposure to As alone, and an increase in the total antioxidant capacity was observed upon exposure to nTiO2 or nTiO2 + As. However, these modulations were not sufficient enough to prevent the lipid damage induced by nTiO2 exposure. Our results suggest that coexposure to nTiO2 and As does not alter the toxicity of this metalloid in the gills and hepatopancreas of L. vannamei but does alter its metabolism, favoring its accumulation of organic As species considered moderately toxic.

  1. Selection for growth performance of tank-reared Pacific white shrimp, Litopenaeus vannamei

    Science.gov (United States)

    Andriantahina, Farafidy; Liu, Xiaolin; Huang, Hao; Xiang, Jianhai

    2013-05-01

    Seven growth-related traits were measured to assess the selection response and genetic parameters of the growth of Pacific white shrimp, Litopenaeus vannamei, which had been domesticated in tanks for more than four generations. Phenotypic and genetic parameters were evaluated and fitted to an animal model. Realized response was measured from the difference between the mean growth rates of selected and control families. Realized heritability was determined from the ratio of the selection responses and selection differentials. The animal model heritability estimate over generations was 0.44±0.09 for body weight (BW), and ranged from 0.21±0.08 to 0.37±0.06 for size traits. Genetic correlations of phenotypic traits were more variable (0.51-0.97), although correlations among various traits were high (>0.83). Across generations, BW and size traits increased, while selection response and heritability gradually decreased. Selection responses were 12.28%-23.35% for harvest weight and 3.58%-13.53% for size traits. Heritability estimates ranged from 0.34±0.09 to 0.48±0.15 for harvest weight and 0.17±0.01-0.38±0.11 for size traits. All phenotypic and genetic parameters differed between various treatments. To conclude, the results demonstrated a potential for mass selection of growth traits in L. vannamei. A breeding scheme could use this information to integrate the effectiveness constituent traits into an index to achieve genetic progress.

  2. Analysis of Litopenaeus vannamei transcriptome using the next-generation DNA sequencing technique.

    Directory of Open Access Journals (Sweden)

    Chaozheng Li

    Full Text Available BACKGROUND: Pacific white shrimp (Litopenaeus vannamei, the major species of farmed shrimps in the world, has been attracting extensive studies, which require more and more genome background knowledge. The now available transcriptome data of L. vannamei are insufficient for research requirements, and have not been adequately assembled and annotated. METHODOLOGY/PRINCIPAL FINDINGS: This is the first study that used a next-generation high-throughput DNA sequencing technique, the Solexa/Illumina GA II method, to analyze the transcriptome from whole bodies of L. vannamei larvae. More than 2.4 Gb of raw data were generated, and 109,169 unigenes with a mean length of 396 bp were assembled using the SOAP denovo software. 73,505 unigenes (>200 bp with good quality sequences were selected and subjected to annotation analysis, among which 37.80% can be matched in NCBI Nr database, 37.3% matched in Swissprot, and 44.1% matched in TrEMBL. Using BLAST and BLAST2Go softwares, 11,153 unigenes were classified into 25 Clusters of Orthologous Groups of proteins (COG categories, 8171 unigenes were assigned into 51 Gene ontology (GO functional groups, and 18,154 unigenes were divided into 220 Kyoto Encyclopedia of Genes and Genomes (KEGG pathways. To primarily verify part of the results of assembly and annotations, 12 assembled unigenes that are homologous to many embryo development-related genes were chosen and subjected to RT-PCR for electrophoresis and Sanger sequencing analyses, and to real-time PCR for expression profile analyses during embryo development. CONCLUSIONS/SIGNIFICANCE: The L. vannamei transcriptome analyzed using the next-generation sequencing technique enriches the information of L. vannamei genes, which will facilitate our understanding of the genome background of crustaceans, and promote the studies on L. vannamei.

  3. Polyculture of Litopenaeus vannamei shrimp and Mugil platanus mullet in earthen ponds

    Directory of Open Access Journals (Sweden)

    Léa Carolina de Oliveira Costa

    2013-09-01

    Full Text Available The objective of the present study was to evaluate the growth performance of the shrimp Litopenaeus vannamei and the mullet Mugil platanus in earthen ponds (200 m² located in the Laboratory of Continental Aquaculture of Universidade Federal do Rio Grande (FURG, in both polyculture and monoculture systems. The study consisted of three replicates, as follows: shrimp monoculture (SM, shrimp and mullet polyculture (PO and mullet monoculture (MM. The stocking density was 10 post-larvae shrimp (PL m−2 and 0.67 mullet m−2. Fish and shrimp were fed commercial shrimp meal (38% crude protein once a day. Initially, the amount of feed to shrimp was 20% of their total biomass which was later reduced to 5%. Mullets were fed at 5% of their stocked biomass. The experiment lasted 79 days during the summer of 2007/2008. At harvest, shrimp in monoculture had weight gain (15.59 g, specific growth rate (8.40% day−1, apparent feed conversion (0.88, survival (91% and production (1.454 kg ha−1 significantly higher than in polyculture (1.039 kg ha−1. Mullets in polyculture had significantly better weight gain (42.72 g and specific growth rate (3.99 % day−1 than those in monoculture (31.04 g and 3.69% day−1, respectively, while the mullet condition factor was significantly smaller in polyculture (1.06 than in monoculture (1.13. The apparent feed conversion of the mullets did not present significant differences between monoculture (2.50 and polyculture (2.40. The physical and chemical water parameters were not significantly different in any of the experiments, except for the transparency, which was higher in earthen ponds with mullet monoculture. Polyculture of shrimp and mullet reared together in earthen ponds negatively affects the shrimp production and favors the production of mullets.

  4. Distribution of trace elements in tissues of shrimp species Litopenaeus vannamei (Boone, 1931) from Bahia, Brazil.

    Science.gov (United States)

    Silva, E; Viana, Z C V; Onofre, C R E; Korn, M G A; Santos, V L C S

    2016-02-01

    In this study, concentrations of trace elements in tissues of shrimp species (Litopenaeus vannamei) from farming and zone natural coastal located in the northeastern Brazil were investigated. The elements determination was performed by optical emission spectrometry with inductively coupled plasma (ICP OES). The following ranges of concentrations in the tissues were obtained in µg g-1 dry weight: Al: 13.4-886.5, Cd: 0.93-1.80; Cu: 24.8-152; Fe: 3.2-410.9; Mn: 0.36-24.4; Se: 0.094-9.81 and Zn: 20.3-109.4. The shrimp muscle can be a good iron source (about 88.9 mg-1g dry weight). The distribution of Se concentration in tissues showed much variation between locations, and the concentration levels found in shrimp muscles of wild samples were high, where its levels in 67% of muscle and 50% of others tissues samples exceeded the ANVISA limit, indicating evidence of selenium bioaccumulation. Significant correlation was observed between the following pairs of elements: Fe-Zn (r= -0.70), Mn-Cu (r= -0.74), Se-Cu (r= -0.68), Se-Mn (r= 0.82) in the muscles; Fe-Al (r= 0.99), Mn-Al (r= 0.62), Mn-Fe (r= 0.62), Se-Al (r = 0.88), Se-Fe (r= 0.87), Se-Mn (r= 0.58) in the exoskeleton and Cu-Zn (r = 0.68), Al-Cu (r= 0.88), Fe-Cu (r= 0.95) and Fe-Al (r= 0.97) in the viscera. PMID:26909636

  5. Effects of different enzymatic hydrolysis methods on the bioactivity of peptidoglycan in Litopenaeus vannamei

    Institute of Scientific and Technical Information of China (English)

    SONG Xiaoling; ZHANG Yue; WEI Song; HUANG Jie

    2013-01-01

    The effects of different hydrolysis methods on peptidoglycan (PG) were assessed in terms of their impact on the innate immunity and disease resistance of Pacific white shrimp,Litopenaeus vannamei.PG derived from Bifidobacterium thermophilum was prepared in the laboratory and processed with lysozyme and protease under varying conditions to produce several different PG preparations.A standard shrimp feed was mixed with 0.05% PG preparations to produce a number of experimental diets for shrimp.The composition,concentration,and molecular weight ranges of the soluble PG were analyzed.Serum phenoloxidase and acid phosphatase activity in the shrimp were determined on Days 6-31 of the experiment.The protective activity of the PG preparations was evaluated by exposing shrimp to white spot syndrome virus (WSSV).Data on the composition of the PG preparations indicated that preparations hydrolyzed with lysozyme for 72 h had more low-molecular-weight PG than those treated for 24 h,and hydrolysis by protease enhanced efficiency of hydrolysis compared to lysozyme.SDS-PAGE showed changes in the molecular weight of the soluble PG produced by the different hydrolysis methods.Measurements of serum phenoloxidase and acid phosphatase activity levels in the shrimp indicated that the PG preparations processed with enzymes were superior to the preparation which had not undergone hydrolysis in enhancing the activity of the two serum enzymes.In addition,the preparation containing more low-molecular-weight PG enhanced the resistance of the shrimp to WSSV,whereas no increased resistance was observed for preparations containing less low-molecular-weight PG.These findings suggest that the immunity-enhancing activity of PG is related to its molecular weight and that increasing the quantity of low-molecular-weight PG can fortify the effect of immunity enhancement.

  6. Influence of dietary carbohydrate on the metabolism of juvenile Litopenaeus stylirostris.

    Science.gov (United States)

    Rosas; Cuzon; Gaxiola; Arena; Lemaire; Soyez; Van Wormhoudt A

    2000-06-28

    The effect of dietary carbohydrates (CBH) on glucose and glycogen, digestive enzymes, ammonia excretion and osmotic pressure and osmotic capacity of Litopenaeus stylirostris juveniles was studied. The increase of CBH, ranging between 1 and 33%, stimulates activities of alpha-amylase and alpha-glucosidase in the hepatopancreas. High levels of glucose in hemolymph and of glycogen in the hepatopancreas were reached at the highest level of dietary CBH; however, the kinetics of accumulation is different. Shrimps fed with low level of CBH needed 3 h to reached glucose peak, whereas only 1 h is necessary for high CBH levels. A saturation curve was observed in glycogen level and alpha-amylase activity with maximum values in shrimp-fed diets containing 21% CBH. This level could be used to be included as a maximum shrimp dietary CBH level. Pre-prandial glycogen levels were observed in shrimp fed a diet containing 1% CBH, indicating an important gluconeogenesis, which affected the protein metabolism. The present results show that a diet containing 10% CBH may not be enough to cover the CBH requirement, which could be satisfied by dietary protein content. The low osmotic capacity observed in shrimp fed on a diet containing 10% CBH coincided with a relatively low post-prandial nitrogen excretion which reflects a low concentration of amino acids circulating in hemolymph, which affected the osmotic pressure and the osmotic capacity. These results reflect the high plasticity of shrimp species to use protein to obtain metabolic energy from food and its limited capacity for processing dietary CBH. PMID:10841934

  7. Litopenaeus vannamei immunestimulated with Macrocystis pyrifera extract:improving the immune response against Vibrio campbellii

    Institute of Scientific and Technical Information of China (English)

    Liliana Noemi Snchez Campos; Fernando Daz Herrera; Ana Denisse Re Araujo; Manuel de Jess Acosta Ruiz

    2014-01-01

    Objective:To examine the immune responses of Litopenaeus vannamei after different treatments with a hot water extract of Macrocystis pyrifera (M. pyrifera) and a subsequent challenge with Vibrio campbellii (V. campbellii). Methods: A total of 184 adult white shrimp that were infected with V. campbellii (1í106 CFU/shrimp) were immunostimulate by the hot-water extract from M. pyrifera via either injection (10 µg) or immersion (350 mg/L), the experimental controls were injected with either saline solution or V. campbellii (1í106 CFU/shrimp). The bacterial DNA depuration rate, antimicrobial activity and total hemocyte count were evaluated in hemolymph samples at 2, 6, 12, 24, 48 and 72 h post-infection. Results:Injected shrimp (10 µg M. pyrifera extract) demonstrated the best clearance of bacterial infection, with 82% survival at 72 h post-infection (cellular response). Hemolymph from the immersed organisms had the best antimicrobial activity against Escherichia coli growth;specifically, the most efficient antimicrobial activity was observed at 24 h post-infection. Both types of immunostimulated shrimp had similar total hemocyte counts at 24 h post-infection (1.63-1.59 million/mL);however, after 72 h, injected shrimp had higher total hemocyte counts than immersed animals (2.59 v.s. 0.56 million/mL). Conclusions: The injection of the M. pyrifera hot-water extract facilitated a more efficient response to V. campbellii infection due to the stimulation of the hemocytes of the shrimp. In other words, the cellular immune response was more efficient to eliminate bacterial infection than the humoral response in shrimp.

  8. Selection for growth performance of tank-reared Pacific white shrimp, Litopenaeus vannamei

    Institute of Scientific and Technical Information of China (English)

    ANDRIANTAHINA Farafidy; LIU Xiaolin; HUANG Hao; XIANG Jianhai

    2013-01-01

    Seven growth-related traits were measured to assess the selection response and genetic parameters of the growth of Pacific white shrimp,Litopenaeus vannamei,which had been domesticated in tanks for more than four generations.Phenotypic and genetic parameters were evaluated and fitted to an animal model.Realized response was measured from the difference between the mean growth rates of selected and control families.Realized heritability was determined from the ratio of the selection responses and selection differentials.The animal model heritability estimate over generations was 0.44±0.09 for body weight (BW),and ranged from 0.21±0.08 to 0.37±0.06 for size traits.Genetic correlations of phenotypic traits were more variable (0.51-0.97),although correlations among various traits were high (>0.83).Across generations,BW and size traits increased,while selection response and heritability gradually decreased.Selection responses were 12.28%-23.35% for harvest weight and 3.58%-13.53% for size traits.Heritability estimates ranged from 0.34±0.09 to 0.48±0.15 for harvest weight and 0.17±0.01-0.38±0.11 for size traits.All phenotypic and genetic parameters differed between various treatments.To conclude,the results demonstrated a potential for mass selection of growth traits in L.vannamei.A breeding scheme could use this information to integrate the effectiveness constituent traits into an index to achieve genetic progress.

  9. Using microsatellite markers to identify heritability of Pacific whiteleg shrimpLitopenaeus vannamei

    Institute of Scientific and Technical Information of China (English)

    ANDRIANTAHINA Farafidy; LIU Xiaolin; HUANG Hao

    2015-01-01

    Pacific whiteleg shrimp (Litopenaeus vannamei) is an economically relevant shrimp species in many Asian countries. The specific objective of the current research was to assess microsatellite markers in screening the fast-growth of domesticatedL. vannamei stocks to establish a founder population for breeding-selection plans. The postlarvae produced by the reproduction of second generation broodstock were cultured in the same conditions throughout a five months growing period. Ninety juvenile shrimp were selected from the slow-, medium- and the fast-growth groups, and ten microsatellite markers were used to investigate their genetic diversity, and to understand the improvement of a breeding-selection scheme. Ten polymorphic loci (markers) (M1–M10) were produced at ten loci in this sample, among them Primer M8 was the highest polymorphic locus and M7 was the lowest one. A specific locus was found in the fast-growth group using Primer M5. The longest genetic distance (0.481) was determined between the fast- and medium-growth groups and the shortest (0.098) was between the slow- and medium-growth groups; therefore, the largest genetic identity (0.946) was observed between the slow-and medium-growth groups and the smallest (0.667) was observed between the medium- and fast-growth groups. The Unweighted Paired Group with Arithmetic Average (UPGMA) dendrogram based on Nei’s genetic distances provided two different groups; the first consist of the slow- and medium-growth groups and the second the fast-growth group. Selection response and realized heritability for growth were 11.55% and 31.26%, respectively. Therefore, this set of microsatellite markers would provide a useful tool in shrimp breeding schemes.

  10. Transcriptome Analysis of the Hepatopancreas in the Pacific White Shrimp (Litopenaeus vannamei) under Acute Ammonia Stress

    Science.gov (United States)

    Lu, Xia; Kong, Jie; Luan, Sheng; Dai, Ping; Meng, Xianhong; Cao, Baoxiang; Luo, Kun

    2016-01-01

    In the practical farming of Litopenaeus vannamei, the intensive culture system and environmental pollution usually results in a high concentration of ammonia, which usually brings large detrimental effects to shrimp, such as increasing the susceptibility to pathogens, reducing growth, decreasing osmoregulatory capacity, increasing the molting frequency, and even causing high mortality. However, little information is available on the molecular mechanisms of the detrimental effects of ammonia stress in shrimp. In this study, we performed comparative transcriptome analysis between ammonia-challenged and control groups from the same family of L. vannamei to identify the key genes and pathways response to ammonia stress. The comparative transcriptome analysis identified 136 significantly differentially expressed genes that have high homologies with the known proteins in aquatic species, among which 94 genes are reported potentially related to immune function, and the rest of the genes are involved in apoptosis, growth, molting, and osmoregulation. Fourteen GO terms and 6 KEGG pathways were identified to be significantly changed by ammonia stress. In these GO terms, 13 genes have been studied in aquatic species, and 11 of them were reported potentially involved in immune defense and two genes were related to molting. In the significantly changed KEGG pathways, all the 7 significantly changed genes have been reported in shrimp, and four of them were potentially involved in immune defense and the other three were related to molting, defending toxicity, and osmoregulation, respectively. In addition, majority of the significantly changed genes involved in nitrogen metabolisms that play an important role in reducing ammonia toxicity failed to perform the protection function. The present results have supplied molecular level support for the previous founding of the detrimental effects of ammonia stress in shrimp, which is a prerequisite for better understanding the molecular

  11. Effect of bioactive peptides (BPs) on the development of Pacific white shrimp ( Litopenaeus vannamei Boone, 1931)

    Science.gov (United States)

    Wang, Guangjun; Yu, Ermeng; Li, Zhifei; Yu, Deguang; Wang, Haiying; Gong, Wangbao

    2016-06-01

    The present study was conducted to evaluate the feasibility of replacing fish meal (FM) with bioactive peptides (BPs) in diet of white shrimp ( Litopenaeus vannamei). The changes in growth performance, body composition, non-specific immunity, and water quality were examined after the shrimp were fed four diets, in which 0% (control), 33.3%, 66.7% and 100% of FM was replaced by BPs, respectively. The groups were designated as Con, 1/3BPs, 2/3BPs, and 3/3BPs. A total of 720 shrimp with an initial body weight of 1.46 ± 0.78 g were fed the experimental diets for 56 days. The results revealed that: 1) the weight gain rate (WGR) in 1/3BPs, 2/3BPs, and 3/3BPs was significantly higher than that in Con ( P body crude protein (CP) and crude lipids (CL) were significantly different among groups, while there was no significant difference between crude ash and phosphorus contents; 3) the levels of acid phosphatase (ACP), lysozyme (LZM), superoxide dismutase (SOD), phenol oxidase (PO) and bactericidal activity increased significantly with the inclusion of BPs; 4) in terms of water quality, no significant difference was found in pH and dissolved oxygen among diets during the whole experimental period. Moreover, even though nitrite and ammonium levels tended to increase with time, there was no significant difference among groups. The results indicated that BPs is an applicable alternative of protein source, which can substitute FM in the diets of L. vannamei; it is able to effectively promote growth performance and improve immunity. Moreover, BPs in the diets had no negative impact on water quality.

  12. Molecular characterization of an adiponectin receptor homolog in the white leg shrimp, Litopenaeus vannamei

    Science.gov (United States)

    Kim, Ah Ran; Alam, Md Jobaidul; Yoon, Tae-ho; Lee, Soo Rin; Park, Hyun; Kim, Doo-Nam; An, Doo-Hae; Lee, Jae-Bong; Lee, Chung Il

    2016-01-01

    Adiponectin (AdipoQ) and its receptors (AdipoRs) are strongly related to growth and development of skeletal muscle, as well as glucose and lipid metabolism in vertebrates. Herein we report the identification of the first full-length cDNA encoding an AdipoR homolog (Liv-AdipoR) from the decapod crustacean Litopenaeus vannamei using a combination of next generation sequencing (NGS) technology and bioinformatics analysis. The full-length Liv-AdipoR (1,245 bp) encoded a protein that exhibited the canonical seven transmembrane domains (7TMs) and the inversed topology that characterize members of the progestin and adipoQ receptor (PAQR) family. Based on the obtained sequence information, only a single orthologous AdipoR gene appears to exist in arthropods, whereas two paralogs, AdipoR1 and AdipoR2, have evolved in vertebrates. Transcriptional analysis suggested that the single Liv-AdipoR gene appears to serve the functions of two mammalian AdipoRs. At 72 h after injection of 50 pmol Liv-AdipoR dsRNA (340 bp) into L. vannamei thoracic muscle and deep abdominal muscle, transcription levels of Liv-AdipoR decreased by 93% and 97%, respectively. This confirmed optimal conditions for RNAi of Liv-AdipoR. Knockdown of Liv-AdipoR resulted in significant changes in the plasma levels of ammonia, 3-methylhistine, and ornithine, but not plasma glucose, suggesting that that Liv-AdipoR is important for maintaining muscle fibers. The chronic effect of Liv-AdipoR dsRNA injection was increased mortality. Transcriptomic analysis showed that 804 contigs were upregulated and 212 contigs were downregulated by the knockdown of Liv-AdipoR in deep abdominal muscle. The significantly upregulated genes were categorized as four main functional groups: RNA-editing and transcriptional regulators, molecular chaperones, metabolic regulators, and channel proteins. PMID:27478708

  13. Fenologia de espécies silvestres de macieira como polinizadora das cultivares gala e fuji Phenology of wild apple species like pollinators of gala and fuji cultivars

    Directory of Open Access Journals (Sweden)

    José Luiz Petri

    2008-12-01

    Full Text Available A escolha correta de cultivares polinizadoras na macieira é determinante para a obtenção de altos rendimentos. Muitas regiões produtoras de maçãs do mundo utilizam macieiras silvestres com o fim específico de polinização, porém existem poucas informações quanto ao uso dessas espécies nas condições climáticas do Sul do Brasil. O objetivo deste trabalho foi avaliar o comportamento fenológico de espécies silvestres de macieira quanto à floração em comparação as cultivares comerciais 'Gala' e 'Fuji', nas condições climáticas do Sul do Brasil, em Caçador-SC (latitude 26º42'32" sul, longitude 51º00'50" oeste e altitude de 960 metros. As macieiras silvestres estudadas foram M. atrosanguinea, M. baccata, M. eleyi, M. floribunda, M. hopa, M. platycarpa, M. robusta, 'John Downil', 'Prof. Spengler', 'Milalew imuni', 'Profusion', 'Winter gold' e 'Yellow Siberian'. As espécies silvestres apresentaram grande variabilidade na época de florescimento e na duração do mesmo ao longo dos anos. A maior coincidência do período de floração e com maior regularidade ao longo dos anos foi obtido entre às cultivares Gala e Fuji. 'Prof. Spengler', 'Profusion', 'Winter gold' e 'John Downil' são as espécies silvestres de macieira com maior potencial de utilização como polinizadoras, podendo ser utilizadas complementarmente para polinização das cultivares Gala e Fuji. As espécies M. hopa, M. eleyi e M. atrosanguinea, devido à alta densidade de floração, podem ser utilizadas como segunda opção para a polinização das cultivares Gala e Fuji.The correct choice of pollinator cultivars is an important management factor for obtaining high productivity in apple trees. Many apple production regions in the world adopt wild apple species with the specific purpose of pollination. However, there is little information about the use of this species in the climatic conditions of South Brazil. The objective of this work was to evaluate the

  14. Natural infection by endoparasites among free-living wild animals Infecção natural por endoparasitas em animais silvestres de vida-livre

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    Luciane Holsback

    Full Text Available The objective of this study was to investigate the frequency of occurrence and variety of intestinal parasites among free-living wild animals. Fecal samples from wild mammals and birds at rehabilitation centers in the states of Mato Grosso do Sul and São Paulo were analyzed by sedimentation and flotation-centrifugation methods. Parasite eggs, oocysts, cysts and/or trophozoites were found in 71% of the samples. Cryptosporidium sp. oocysts were detected in fecal samples from oncillas (Leopardus tigrinus and scaly-headed parrots (Pionus maximiliani. Giardia cysts were identified in the feces of a gray brocket (Mazama gouazoubira. Among the most common parasites found, there were eggs from Toxocara cati, Toxascaris leonina and Ancylostoma tubaeforme, and from Cestoda. Several Enterobius sp. eggs were found in the feces of red howler monkeys (Alouatta seniculus. It can be concluded from this study that despite the small number of samples, the diversity of parasites found was noteworthy. Additional information about parasite endofauna in wild animals is needed, since their presence might suggest that there could be proximity to and interactions with domestic animals and/or humans. In addition, further studies on parasites from free-living wild animals are of prime importance for understanding the intensity of anthropic changes in wild environments.O objetivo deste trabalho foi pesquisar a frequência de ocorrência e a variedade de parasitas intestinais de animais silvestres de vida livre. Amostras de fezes de mamíferos e aves silvestres de centros de reabilitação dos Estados do Mato Grosso do Sul e São Paulo, foram analisadas pelos métodos de sedimentação e de centrífugo-flutuação. Foram encontrados ovos, oocistos, cistos e/ou trofozoítos de parasitas em 71% das amostras. Oocistos de Cryptosporidium sp. foram detectados em amostras de fezes de gato-do-mato-pequeno (Leopardus tigrinus e maritacas (Pionus maximiliani. Cistos de Giardia

  15. PARÁMETROS HEMATOLÓGICOS EN POLLUELOS DE PSITÁCIDOS EN CAUTIVERIO DE ORIGEN SILVESTRE

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    J. A. Herrera

    2013-01-01

    Full Text Available El objetivo de este estudio fue describir los parámetros hematológicos en 69 polluelos en cautiverio de origen silvestre, clínicamente sanos, menores de tres meses de edad, en cuatro especies de psitácidos: Pionus senilis (n = 18, Amazona albifrons (n = 15, Amazona autumnalis (n = 25 y Amazona oratrix (n = 11, procedentes de la región central de México. Para determinar dichos parámetros, de cada ejemplar se obtuvieron 500 μl de sangre por punción de la vena braquial. En las cuatro especies citadas, el porcentaje del hematocrito fue de 56,4 ± 3,36; 51 ± 6,52; 47,6 ± 6,47 y 53,8 ± 9,26; el VCM en fl fue de 184,6 ± 13,4; 142,0 ± 25,4; 106,3 ± 11,7 y 162,3 ± 71,9; la cuenta eritrocítica fue de 4,7 ± 2,1 x10 6 /μl; 6,1 ± 8,2 x10 6 /μl; 6,9 ± 1,2 x10 6 /μl y 5,4 ± 1,1 x10 6 /μl; y la cuenta leucocitaria de 3,06 ± 0,15 x10 3 /μl; 3,62 ± 0,24 x10 3 /μl; 4,48 ± 0,36 x10 3 /μl y 3,56 ± 0,71 x10 3 /μl respectivamente para P. senilis , A. albifrons , A. autumnalis y A. oratrix . Se constató tendencia a la heterofilia en A. albifrons y A. oratrix , lo cual puede ser de gran utilidad para un diagnóstico cada vez más preciso en la clínica de aves en cautiverio.

  16. Teste de ELISA indireto para diagnóstico sorológico de leishmaniose visceral em canídeos silvestres

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    Paulo R.B. Ferreira

    2013-04-01

    Full Text Available Na América do Sul, alguns canídeos silvestres são considerados reservatórios naturais da Leishmania chagasi. A resposta imunológica desses animais à Leishmania é pouco conhecida, havendo a necessidade de métodos diagnósticos adequados para esse fim. No presente estudo, é descrita a padronização do ensaio imunoenzimático indireto (ELISA para o diagnóstico sorológico de leishmaniose visceral em canídeos silvestres brasileiros. Foram estudadas amostras de soro e plasma de 12 canídeos cativos: sete lobos-guará (Chrysocyon brachyurus, três raposinhas (Lycalopex vetulus e dois cachorros-do-mato (Cerdocyon thous. As amostras de um C. brachyurus e uma L. vetulus, cativos em área endêmica para LV, que apresentavam doença clínica e positividade em testes de Imunofluorescência Indireta e Reação em Cadeia de Polimerase, foram utilizadas como controles positivos. Foram comparados os conjugados anti-IgG de cão e proteína A, ambos ligados a peroxidase, cujos testes detectaram quatro (04/12 e três (03/12 C. brachyurus soropositivos para anticorpos anti-Leishmania sp., respectivamente. As médias das densidades ópticas (DOs das amostras negativas foram nitidamente mais baixas do que as médias das DOs dos positivos tanto no ELISA com anti-IgG de cão (4,8 vezes como com proteína A (15,5 vezes. Os soros de três C. brachyurus positivos no ELISA indireto foram avaliados por Western blotting e identificaram 22 bandas, sendo imunodominantes as de peso molecular de 19, 22, 24, 45 e 66 kDa. Os testes ELISA com a proteína A e o conjugado anti-IgG de cão apresentaram respectivamente concordância excelente (Kappa = 1; p<0,001 e moderada (Kappa = 0,8; p<0,0015, com o Western blotting. Ambos foram, portanto, considerados adequados a avaliações de triagem de animais cuja resposta humoral de anticorpos indica contato com o parasito, úteis para subsidiar estudos para adequação de metodologias específicas para os canídeos silvestres.

  17. Detección de Rickettsia spp. en Suero y Garrapatas de Mamíferos Silvestres en Cautiverio en Montería, Córdoba -resumen-

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    Dave Wehdeking-Hernández

    2014-12-01

    Full Text Available Las rickettsiosis transmitidas por garrapatas son causadas por bacterias intracelulares pertenecientes al grupo de las fiebres manchadas del género Rickettsia. Estas zoonosis son algunas de las enfermedades transmitidas por vectores de más antiguo conocimiento‚ e incluyen dentro de su ciclo de transmisión diversas especies de mamíferos. No obstante‚ en Colombia existen pocos trabajos enfocados a conocer la dinámica de las rickettsiosis en animales silvestres. El objetivo de este estudio fue detectar‚ por medio de la técnica de PCR en tiempo real‚ la presencia de Rickettsia spp.‚ en muestras de suero y garrapatas colectadas en mamíferos mantenidos en cautiverio en el Centro de Atención y Valoración de Fauna Silvestre de la Corporación autónoma Regional de los Valles del Sinú y San Jorge CAV-CVS‚ entre los años 2009 y 2014. Se obtuvieron muestras de sangre de 14 felinos‚ de las especies Leopardus pardalis (10‚ Puma concolor (3 y Panthera onca (1; de 58 primates‚ correspondientes a las especies Ateles geofroyii (17‚ Alouatta seniculus (14‚ Cebus capucinus (14‚ Saguinus oedipus (6‚ Cebus albifrons (4 Aotus sp. (2 y Ateles belzebuth (1; y de otros mamíferos: Tayassu pecari (1‚ Procyon cancrivorous (1 y Cerdocyon thous (1. En cuanto a las garrapatas‚ se colectaron e identificaron individuos del género Haemaphysalis sp. en un ejemplar de Sylvilagus sp.‚ y de las especies Amblyomma ovale‚ Riphicephalus microplus y Dermacentor nitens en P. concolor. Posteriormente fueron organizadas en pool. No se encontraron muestras de suero positivas a Rickettsia spp. (0/72. Se detectó un pool de garrapatas de la especie A. ovale positivo a este microorganismo. Este es el primer reporte en Colombia de ectoparásitos de Puma concolor positivos a Rickettsia spp. Se requiere continuar con los muestreos de ectoparásitos para determinar si este microorganismo se encuentra circulante entre mamíferos silvestres en el país.

  18. New records of Histoplasma capsulatum from wild animals in the Brazilian Amazon Novos registros de Histoplasma capsulatum em animais silvestres na Amazônia brasileira

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    Roberto Daibes Naiff

    1996-08-01

    Full Text Available Twenty-eight isolates of Histoplasma capsulation were obtained from eight species of forest mammals from the States of Amazonas, Pará and Rondônia in the Amazon Region of Brazil. Primary isolates were obtained by inoculating triturated liver and spleen tissue intradermally and intraperito-neally in hamsters. Mycological diagnosis in hamsters presenting lesions was confirmed by histopathology and culture on Sabouraud dextrose-agar. Infected hamsters developed signs of disease within two to nine months; all had disseminated visceral lesions and most also had skin lesions at the sites of inoculation. None of the hamsters inoculated with skin macerates of the original hosts developed histoplasmosis, and histopathological examination of the viscera of the wild hosts failed to reveal H. capsulation. Prevalence of infection was considerably higher in females than in males both for the opossum Didelphis marsupialis and for total wild animals (479 examined. It is proposed that canopy-dwelling mammals may acquire the infection from conidia borne on convective currents in hollow trees with openings at ground-level.Vinte e oito amostras de Histoplasma capsulatum foram obtidas de oito espécies de mamíferos silvestres nos Estados do Amazonas, Pará e Rondônia. Os isolamentos foram feitos mediante inoculação de amostras trituradas de fígado e baço em hamsters por via intradérmica e intraperitoneal. O diagnóstico micológico nos hamsters que apresentaram lesões foi confirmado por histopatologia e cultivo em meio dextrose-agar de Sabouraud. Os hamsters infectados desenvolveram sinais de doença após dois a nove meses; todos apresentaram lesões disseminadas nas vísceras e a maioria apresentou também lesões cutâneas nos locais da inoculação. Nenhum dos hamsters inoculados com material de pele dos hospedeiros originais desenvolveu histoplasmose, e H. capsulatum não foi detectado nos exames histopatológicos dos animais silvestres. A preval

  19. CRESCIMENTO E SOBREVIVÊNCIA DO CAMARÃO BRANCO DO PACIFÍCO Litopenaeus vannamei (BOONE, 1931 EM DIFERENTES SALINIDADES GROWTH AND SURVIVAL OF PACIFIC WHITE SHRIMP Litopenaeus vannamei (BOONE, 1931 IN DIFFERENT SALINITIES

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    Carlos Henrique dos Anjos dos Santos

    2009-09-01

    Full Text Available O presente estudo teve como objetivo avaliar o desenvolvimento de pós-larvas de Litopenaeus vannamei cultivadas em diferentes salinidades. Realizou-se o experimento no Centro de Tecnologia em Aquicultura da Universidade Federal do Ceará, durante o período de 1.o de setembro de 2006 a 31 de outubro de 2006. Em vinte aquários com volume útil de 30L foram distribuídos, em um delineamento inteiramente casualizado, cinco tratamentos (controle – 35%, 30‰, 20‰, 10‰ e 0‰ com quatro repetições cada. Foi utilizada a densidade de trinta indivíduos/aquário. As pós-larvas, em fase de PL 10, apresentavam peso e comprimento médio total inicial de 0,008 ± 0,001g e 11,00 ± 0,05mm, respectivamente. Os animais foram, gradativamente, aclimatados nas salinidades experimentais. Para as análises estatísticas, aplicou-se a análise de variância (ANOVA e, posteriormente, caso se verificassem diferenças significativas entre os tratamentos, o teste Tukey de separação de médias (? = 0,001. Os parâmetros de qualidade de água analisados mantiveram-se dentro dos padrões aceitáveis para o cultivo da espécie. As pós-larvas cultivadas em 0‰ apresentaram os melhores valores de peso final, incremento em peso, incremento relativo diário de biomassa e taxa de sobrevivência (P<0,001. Todavia, os animais cultivados em 10% mostraram os melhores valores de comprimento total e incremento em comprimento (P<0,001. Os resultados do presente estudo sugerem que pós-larvas de L. vannamei apresentam taxas similares de crescimento e sobrevivência quando cultivadas em diferentes salinidades.

    PALAVRAS-CHAVES: Gradiente salino, laboratório Litopenaeus vannamei, osmorregulação.
    The aim of this work was to evaluate the development of Litopenaeus vannameiat post-larvae (PL at different salinities. This experiment was carried out at the “Centro de Tecnologia em Aqüicultura/UFC” at the period from 01/09/2006 to 31/10/2006. Twenty

  20. Efecto de la salinidad sobre la fisiología energética del camarón blanco Litopenaeus vannamei (Boone) Effect of salinity on physiological energetics of white shrimp Litopenaeus vannamei (Boone)

    OpenAIRE

    Gustavo Valdez; Fernando Díaz; Ana Denisse Re; Elizabeth Sierra

    2008-01-01

    Se determinó el balance energético en juveniles de Litopenaeus vannamei, aclimatados a la salinidad como hiperosmóticos (20 ups), isosmóticos (26 ups), o hiposmóticos (32 ups). Además, se calculó la razón atómica O:N. La tasa de ingestión, el consumo de oxígeno, la excreción de amonio y el campo de crecimiento fueron afectados significativamente (p < 0.05) cuando los organismos fueron aclimatados a 20, 26 y 32 ups. La mayor cantidad de energía ingerida contenida en el alimento se determinó en...

  1. Regulation of water quality and growth characteristics of indoor raceway culture of Litopenaeus vannamei

    Institute of Scientific and Technical Information of China (English)

    ZANG Weiling; YANG Ming; DAI Xilin; HOU Wenjie; LIU Yongshi; DING Fujiang

    2009-01-01

    Two modes of regulating the water quality of experimental ponds in indoor raceway culture of Litopenaeus vannamei were evaluated using simple water treatment facilities. A self-made water purifying net, aeration stone, composite microbe preparation, and Ceratophyllum demersum were placed in the experimental ponds and the culture water was circulated along the raceway inside the pond using a paddle wheel aerator. In addition, the water quality in the experimental pond was improved by draining effluent from the pipeline at the bottom of ponds 7 and 8 (mode I) and exchanging the circulating water in pond 10 (mode II) with the reservoir water in pond 9 using a pump and pipeline. The water quality in the experimental ponds was similar in response to regulation using mode I or mode II. Water quality parameters in the experimental ponds were controlled within a suitable range by simple facilities during culture period without using any chemical treatments. The rich content of dissolved oxygen was maintained by the circular flow and continuous aeration of the pond water. The respective average values of the main water parameters in experimental ponds 7 and 10 in response to regulation of the water quality using modes I and II were as follows: pH 8.17 and 7.99; DO 5.16 mg/L and 5.97 mg/L; CODMn 18.45 and 12.61 mg/L; TAN (NH_3-N) 0.854 mg/L (0.087 mg/L) and 0.427 mg/L (0.012 mg/L); NO_2-N 0.489 mg/L and 0.337 mg/L. Moreover, the average body length and body weight of harvested shrimp of pond 7 and pond 10 were 7.56 cm and 8.99 cm, 5.10 g and 8.33 g, respectively. Furthermore, the survival rate, average biomass yield and average condition factor of the shrimp harvested were 70% and 60%, 2.54 kg/m~2 and 2.14 kg/m~2, and 0.675 g/cm and 0.927 g/cm, respectively. Linear equations describing the relationship between body length and culture time and cubic or power functions describing the relationship between body weight and body length were obtained based on evaluation of the

  2. The known two types of transglutaminases regulate immune and stress responses in white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Chang, Chin-Chyuan; Chang, Hao-Che; Liu, Kuan-Fu; Cheng, Winton

    2016-06-01

    Transglutaminases (TGs) play critical roles in blood coagulation, immune responses, and other biochemical functions, which undergo post-translational remodeling such as acetylation, phosphorylation and fatty acylation. Two types of TG have been identified in white shrimp, Litopenaeus vannamei, and further investigation on their potential function was conducted by gene silencing in the present study. Total haemocyte count (THC), differential haemocyte count (DHC), phenoloxidase activity, respiratory bursts (release of superoxide anion), superoxide dismutase activity, transglutaminase (TG) activity, haemolymph clotting time, and phagocytic activity and clearance efficiency to the pathogen Vibrio alginolyticus were measured when shrimps were individually injected with diethyl pyrocarbonate-water (DEPC-H2O) or TG dsRNAs. In addition, haemolymph glucose and lactate, and haemocytes crustin, lysozyme, crustacean hyperglycemic hormone (CHH), transglutaminaseI (TGI), transglutaminaseII (TGII) and clotting protein (CP) mRNA expression were determined in the dsRNA injected shrimp under hypothermal stress. Results showed that TG activity, phagocytic activity and clearance efficiency were significantly decreased, but THC, hyaline cells (HCs) and haemolymph clotting time were significantly increased in the shrimp which received LvTGI dsRNA and LvTGI + LvTGII dsRNA after 3 days. However, respiratory burst per haemocyte was significantly decreased in only LvTGI + LvTGII silenced shrimp. In hypothermal stress studies, elevation of haemolymph glucose and lactate was observed in all treated groups, and were advanced in LvTGI and LvTGI + LvTGII silenced shrimp following exposure to 22 °C. LvCHH mRNA expression was significantly up-regulated, but crustin and lysozyme mRNA expressions were significantly down-regulated in LvTGI and LvTGI + LvTGII silenced shrimp; moreover, LvTGII was significantly increased, but LvTGI was significantly decreased in LvTGI silenced shrimp

  3. Does zero-water discharged technology enhance culture performance of pacific white shrimp (Litopenaeus vannamei Boone.)?

    Science.gov (United States)

    Suantika, Gede; Anggraeni, Jayanty; Hasby, Fahri Azhari; Yanuwiarti, Ni Putu Indah

    2014-03-01

    Litopenaeus vannamei or white leg shrimp is an introduced shrimp which has successfully cultured in Indonesia. In Indonesia, L. vannamei is commonly cultured on outdoor/earthen pond that requires renewal of water, less control in term of water quality and disease and attributed to unpredictable yield production. Based on the existing culture condition, a system that enable to minimize water consumption, improve the hygiene of the culture and at the same time maintain a more stable yield production is urgent to be developed by using a zero water discharge system. The system consists of: (a) culture tank - to retain and culture the shrimp; (b) CaCO3 grained - buffering agent and substrate of nitrifying bacteria; (c) aeration line - to provide O2 and homogenize the culture; (d) ancho (feeding) - to control an appropriate feed; (e) nitrifying bacteria adding - to consume ammonium and nitrite then convert it to nitrate, and also control pathogen Vibrio sp.; (f) diatom microalgae (Chaetoceros gracilis) - to uptake nitrate, bacteriostatic agent, feed source, provide O2 and shading. In this study, there were 2 treatments: the static culture (batch) system was set as control (K) (in 70 PL/m2), and culture system with zero-water discharge system which was inoculated by 0.02% v/v 106 CFU/ml of mixed culture nitrifying bacteria and diatom microalgae in 70 PL/m2 (P1). The white leg shrimp used in this experiment was at post larvae (PL) 10 and cultured in a batch system (1 × 1 × 0.5 m3 pond) during 2 months. Several parameters including survival rate, mean body weight, and water quality (salinity, temperature, pH, DO, ammonium, nitrite, and nitrate) were measured. Based on the results, biomass of P1 (237.12 ± 31.11) gram is significantly higher than control (K) (180.80 ± 12.26) gram (Pnitrifying bacteria and diatom microalgae can improve productivity of white shrimp by increasing the biomass and maintaining a stable water quality especially ammonium concentration.

  4. Steroid hormone "cortisone" and "20-hydroxyecdysone" involved in the non-specific immune responses of white shrimp (Litopenaeus vannamei).

    Science.gov (United States)

    Wu, Yu-Sheng; Chang, Ching-Hsu; Nan, Fan-Hua

    2016-09-01

    This study investigated the effect of two steroid hormones on phenoloxidase activity, O2(-) production in the haemocytes, total haemocyte count (THC), superoxide dismutase (SOD) activity, glutamate oxaloacetate transaminase (GOT) activity, glutamate pyruvate transaminase (GPT) activity, and plasma cholesterol concentrations in white shrimps (Litopenaeus vannamei). Phenoloxidase activity, THC and plasma cholesterol concentration in shrimps treated with cortisone and 20-hydroxyecdysone were found to be lower when compared with the control groups. In the observation of O2(-) production, treatment of cortisone and hydroxyecdysone were reducing the activity in the 1st day, but to be undiversified with the elapsed time. By contrast, SOD activity in the hepatopancreas, plasma GOT activity, and GPT activity were significantly increased when compared with the control groups. PMID:27403594

  5. Evaluation of the bioaccumulation of heavy metals in white shrimp (Litopenaeus vannamei) along the Persian Gulf coast.

    Science.gov (United States)

    Dadar, Maryam; Peyghan, Rahim; Memari, Hamid Rajabi

    2014-09-01

    The concentrations of heavy metals in Persian Gulf are low, but petrochemical and refinery activities have caused an increase in heavy metal wastes, especially in coastal regions. The present study was done to determine the bioaccumulation of heavy metals in the muscle of white shrimp (Litopenaeus vannamei) using flame atomic absorption spectrophotometry. The experiment was conducted in four important coastal regions of the Persian Gulf: Bushehr, Deylam, Mahshahr, and Abadan. Amounts of seven heavy metals such as Copper (Cu), Iron (Fe), Lead (Pb), Zinc (Zn), Nickel (Ni), Cadmium (Cd), and Cobalt (Co), were measured as µg/g heavy metal in dry weight in the muscle of white shrimp from the afore-mentioned regions during 2011. This study revealed information that the primary risk for human health and the marine life chain was lead in the muscles of white shrimp in Mahshahr, where intense petrochemical and refinery activities are conducted. Concentrations of other heavy metals were lower than world standards.

  6. Effect of inactive yeast cell wall on growth performance, survival rate and immune parameters in Pacific White Shrimp (Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Rutchanee Chotikachinda

    2008-10-01

    Full Text Available Effects of dietary inactive yeast cell wall on growth performance, survival rate, and immune parameters in pacific white shrimp (Litopenaeus vannamei was investigated. Three dosages of inactive yeast cell wall (0, 1, and 2 g kg-1 were tested in three replicate groups of juvenile shrimps with an average initial weight of 7.15±0.05 g for four weeks. There was no significant difference in final weight, survival rate, specific growth rate, feed conversion ratio, feed intake, protein efficiency ratio, and apparent net protein utilization of each treatments. However, different levels of inactive yeast cell wall showed an effect on certain immune parameters (p<0.05. Total hemocyte counts, granular hemocyte count, and bacterial clearance were better in shrimp fed diets supplemented with 1 and 2 g kg-1 inactive yeast cell wall as compared with thecontrol group.

  7. Management measures to control diseases reported by tilapia (Oreochromis spp.) and whiteleg shrimp (Litopenaeus vannamei) farmers in Guangdong, China

    DEFF Research Database (Denmark)

    Li, Kang; Liu, Liping; Clausen, Jesper Hedegaard;

    2016-01-01

    Culture of tilapia (Oreochromis spp.) and whiteleg shrimp (Litopenaeus vannamei) has intensified during the last decade in China with increased production, meanwhile it has also brought some problems, including diseases, increased use of antimicrobials and other chemicals for disease control...... and pond water quality management. This study investigated the knowledge, practices and challenges of tilapia and whiteleg shrimp farmers when preventing and controlling diseases through the use of antimicrobials and other compounds in Guangdong province, which is the most important shrimp and tilapia...... in recent years. Shrimp farms (30) mainly experienced outbreaks of red body disease (19) and white spot syndrome (5), both viral diseases, and so-called “secret death disease” (5) which farmers controlled by application of a variety of disinfectants, probiotics and vitamins. Most of the farmers reported...

  8. Effects of water temperature and dietary carbohydrate levels on growth and energy budget of juvenile Litopenaeus vannamei

    Institute of Scientific and Technical Information of China (English)

    WANG Xingqiang; MA Shen; DONG Shuanglin

    2006-01-01

    A 3×3 factorial experiment was conducted to determine the effects of water temperature (22 ℃, 27℃ and 32℃) and dietary carbohydrate (CBH) levels (15.47%, 29.15% and 41.00%) on growth, food consumption, feed efficiency, apparent digestibility coefficient and energy budget of juvenile Litopenaeus vannamei. The results showed that, at each dietary CBH level, specific growth rate, food consumption and apparent digestibility coefficient generally increased, while feed efficiency decreased with increasing water temperatures. Specific growth rate and food consumption were the highest in the shrimps fed with diet of 29.15% CBH, closely followed by those with 15.47% CBH, and those with 41.00% CBH had the lowest value.

  9. Caracterización morfológica de accesiones silvestres de guayaba Morphologic characterization of wild accesions of guava

    Directory of Open Access Journals (Sweden)

    Liliana Jiménez Lozano

    2009-04-01

    Full Text Available Se realizó la caracterización morfológica de 22 accesiones silvestres de guayaba Psidium guajava L. 14 colectadas en el municipio de Restrepo (Valle del Cauca, seis en Armenia (Quindío y dos en Pereira (Risaralda. Se utilizaron 12 descriptores cuantitativos y 10 cualitativos de tallo, hojas y frutos; el análisis de agrupamiento se hizo mediante el coeficiente de Dice-Nei-Li y el promedio aritmético no ponderado (UPGMA. La mayor variabilidad se halló en los descriptores peso de la pulpa (CV = 55.92%, peso (CV = 45.23%, y acidez del fruto (CV = 44.75%. El análisis de agrupamientos con base en caracteres cuantitativos permitió establecer cuatro grupos: las accesiones del grupo A (Armenia, presentaron valores promedio de los descriptores de la calidad del fruto (grados Brix, acidez del fruto y relación grados Brix/acidez y valores altos de contenido de pulpa. La mayoría de accesiones del grupo C (Restrepo tuvieron altos valores de calidad del fruto y bajo contenido de pulpa. El grupo B, constituido por accesiones de Armenia y Pereira, se diferenció por valores bajos en los descriptores de rendimiento del fruto (peso del fruto, de la pulpa y diámetro de la cavidad seminal. Las accesiones del grupo D (Restrepo mostraron valores promedios en calidad y rendimiento del fruto. Los descriptores cuantitativos se reunieron en tres variables sintéticas para rendimiento y calidad del fruto que representaron 76.86% de la variabilidad total.Quantitative and qualitative morphologic characterization of 22 wild accessions of guava Psidium guajava L. collected in Restrepo (Valle del Cauca, Armenia (Quindío and Pereira (Risaralda was done. Twelve (12 quantitative and ten (10 qualitative descriptors of stem, leaves and fruits were used. The Dice- Nei Li coefficient and the UPGMA was used for the cluster analysis. Dendrograms and principal components analysis were used. The highest variability was associated with fruit descriptors, pulp weight (CV = 55

  10. Rastreio parasitológico em populações de caprinos silvestres, assilvestrados e domésticos no Parque Nacional da Peneda-Gerês

    OpenAIRE

    Figueiredo, Patrícia de Jesus

    2011-01-01

    Dissertação de Mestrado Integrado em Medicina Veterinária A cabra-montesa (Capra pyrenaica) é uma espécie endémica da Península ibérica, uma das mais emblemáticas do Parque Nacional da Peneda-Gerês. Contam-se 13 anos desde o regresso deste ungulado silvestre a território português depois de a espécie ser considerada extinta em 1890. A presença crescente de gado doméstico e assilvestrado, sobretudo de caprinos, nas áreas ocupadas pelas cabras montesas constitui uma das principais ameaças...

  11. Reproducción de cochinilla silvestre Dactylopius opuntiae (Homóptera: Dactylopiidae Reproduction of wild cochineal Dactylopius opuntiae (Homoptera: Dactylopiidae

    Directory of Open Access Journals (Sweden)

    Arnoldo Flores-Hernández

    2006-06-01

    Full Text Available Por sus características, la cochinilla silvestre ofrece perspectivas de aprovechamiento como fuente de carmín y para el mejoramiento genético de la grana fina. Por lo anterior, se estableció una multiplicación y crianza de cochinilla silvestre, proveniente de la zona árida del norte de México para caracterizarla, utilizando como hospedero a Opuntia megacantha Salm Dyck. Los resultados indican que la especie silvestre pertenece al género-especie Dactylopius opuntia. Se determinó la presencia de partenogénesis en hembras. La duración de los estadios biológicos depende del sexo del insecto; el adulto hembra permaneció durante 38.4 días y 4.2 días para machos, los primeros estadios ninfales fueron similares en duración (18.1-19.8 días. El ciclo biológico de las hembras fue de 77 días mientras que el de los machos fue de 43 días. Para las hembras se estimó un periodo de preoviposición de 18.8 días, manteniéndose en oviposición durante 21 días con un promedio de 131 insectos por hembra. La proporción sexual hembras: machos fue 1:1. El tipo de reproducción fue predominantemente sexual, aunque hubo hembras partenogénicas. Este es el primer reporte de Dactylopius opuntiae como cochinilla silvestre asociada a la zona árida del noreste de México, específicamente en el Bolsón de Mapimí, Durango, México.Wild cochineal has characteristics that offer advantage as a source of carmine and for fine cochineal improvement. To characterize wild cochineal, we initiated a breeding effort raising wild cochineal from the arid zone of the north oh Mexico, using as a host organism Opuntia megacantha Salm dick. The results indicate that the wild specie is Dactylopius opuntiae. The presence of parthenogenesis in females was determined. The duration of ontogenetic stages depends on the sex of the insect. The adult female lasted 38.4 days and 4.2 days for males, the first stage nymphs were similar in duration (18-19.8 days. The complete

  12. Arbovírus Ilheus em aves silvestres (Sporophila caerulescens e Molothrus bonariensis) Ilheus arbovirus in wild birds (Sporophila caerulescens and Molothrus bonariensis)

    OpenAIRE

    Luiz Eloy Pereira; Akemi Suzuki; Terezinha Lisieux Moraes Coimbra; Renato Pereira Souza; Esther Luiza Bocato Chamelet

    2001-01-01

    OBJETIVO: Relatar o isolamento do vírus Ilheus no Estado de São Paulo e avaliar o seu impacto para a saúde pública. MÉTODOS: O isolamento de vírus foi realizado em camundongos albinos Swiss, a partir de sangue de aves silvestres, capturadas com redes de espera tipo mist net, armadas no nível do solo, no Parque Ecológico do Tietê, São Paulo. A identificação das cepas isoladas foi feita pelos testes de inibição da hemaglutinação, fixação de complemento e neutralização em camundongos. Amostras d...

  13. A ten-month diseases survey on wild Litopenaeus setiferus (Decapoda: Penaeidae) from Southern Gulf of Mexico.

    Science.gov (United States)

    del Río-Rodríguez, Rodolfo Enrique; Pech, Daniel; Soto-Rodriguez, Sonia Araceli; Gomez-Solano, Monica Isela; Sosa-lopez, Atahualpa

    2013-09-01

    The development of shrimp aquaculture in Mexican coasts of the Gulf of Mexico began to be explored using the Pacific white shrimp Litopenaeus vannamei in the mid 90's. Many concerns over the risk of disease transmission to the economically important native penaeids, have been the main deterrent for the aquaculture of L. vannamei in the region. Concurrently, more than 10 years of research experience on the aquaculture suitability of the native Litopenaeus setiferus from the Terminos Lagoon, in the Yucatán Peninsula, have been accumulated. The aim of this study was then to determine the seasonal variations of the naturally acquired diseases and the possible detection of exotic pathogens. For this, random subsamples (n-60) of juveniles L. setiferus were collected from monthly captures. In order to detect the widest range of pathogens, including infectious hypodermal and hematopoietic necrosis (IHHNV) and white spot syndrome (WSSV) viruses, both histopathological and molecular methods were employed. Monthly prevalence (%) was calculated for every finding. We were able to detect a total of 16 distinct histological anomalies, most of which the presumptive aetiological agent was readily identified. PCR results for viruses were negative. For some pathogens and symbionts, the prevalence was significantly different between the adult and juvenile populations. Prevalence of diseases tended to be higher in juvenile shrimp than in adults. The results of this study indicated that L. setiferus carry a wide variety of pathogens and symbionts that seem to be endemic to penaeids of the Gulf of Mexico, and those juveniles were more conspicuous to acquire pathogens and symbionts than adults. PMID:24027916

  14. CARACTERIZACIÓN MORFOAGRONÓMICA DEL GERMOPLASMA CUBANO DE PAPA (Solanum ssp. EVALUACIÓN DE LAS ESPECIES SILVESTRES. PARTE I

    Directory of Open Access Journals (Sweden)

    J. G. Castillo

    2007-01-01

    Full Text Available En el Instituto Nacional de Ciencias Agrícolas, durante las campañas 2002-2003, 2003-2004 y 2004-2005, se estudiaron 63 especies silvestres del germoplasma cubano de papa. Se realizó una caracterización morfoagronómica de las especies del banco, que incluyó los siguientes caracteres cualitativos: forma del tubérculo, color del tubérculo y profundidad de los ojos así como cuantitativos: rendimiento por planta (t.ha-1, masa promedio del tubérculo, número de tubérculos por planta y altura de la planta. Además, se evaluó el comportamiento ante el hongo A. solani y el virus del enrollamiento de la hoja (PLRV. Los caracteres cualitativos se expresan en porcentajes de presencia dentro de la colección. Para los cuantitativos se determinaron los estadísticos descriptivos, media, valores máximos y mínimos, desviación estándar y coeficiente de variación. Se realizó un análisis de componentes principales, para conocer la distribución de cada una de las especies dentro de la colección y su posible uso en el programa de mejoramiento cubano. Se encontró una alta variabilidad dentro de esta colección de especies silvestres para las condiciones cubanas, así como las fuentes de resistencia para el hongo A. solani y el PLRV. Se demostró, mediante esta caracterización, que existe un amplio pool de genes en esta colección, para ser utilizada más ampliamente en el programa de mejoramiento cubano.

  15. Salinity tolerance, ontogeny of osmoregulation and zootechnical improvement in the larval rearing of the Caledonian Blue Shrimp, Litopenaeus stylirostris (Decapoda, Penaeidae)

    OpenAIRE

    Pham, Dominique; Charmantier, Guy; Wabete, Nelly; Boulo, Viviane; Broutoi, Francis; Mailliez, Jean-rene; Peignon, Jean-marie; Charmantier-Daures, Mireille

    2012-01-01

    The ontogeny of osmoregulationwas investigated in Litopenaeus stylirostris by studying salinity tolerance and osmoregulatory capacity. Shrimp at different larval and postlarval stages were exposed to various salinities and survival was monitored for 24 h. Survival rates exceeded 80% at salinity over 25 ppt (750 mOsm.kg(-1)) at all the stages. At salinities below to 25 ppt, salinity tolerance was higher in nauplii and zoeae than in mysis larvae. Postlarval stages were able to withstand lower s...

  16. Correlation between Detection of a Plasmid and High-Level Virulence of Vibrio nigripulchritudo, a Pathogen of the Shrimp Litopenaeus stylirostris▿

    OpenAIRE

    Reynaud, Yann; Saulnier, Denis; Mazel, Didier; Goarant, Cyrille; Le Roux, Frédérique

    2008-01-01

    Vibrio nigripulchritudo, the etiological agent of Litopenaeus stylirostris summer syndrome, is responsible for mass mortalities of shrimp in New Caledonia. Epidemiological studies led to the suggestion that this disease is caused by an emergent group of pathogenic strains. Genomic subtractive hybridization was carried out between two isolates exhibiting low and high virulence. Our subtraction library was constituted of 521 specific fragments; 55 of these were detected in all virulent isolates...

  17. Grow-out of sandfish Holothuria scabra in ponds shows that co-culture with shrimp Litopenaeus stylirostris is not viable

    OpenAIRE

    Bell, J.; Agudo, N.; Purcell, S.; Blazer, P; Simutoga, M; Pham, Dominique; Della Patrona, Luc

    2007-01-01

    We examined the potential for producing the large numbers of sandfish (Holothuria scabra) needed for restocking programmes by co-culturing juveniles with the shrimp Litopenaeus stylirostris in earthen ponds. Our experiments in hapas within shrimp ponds were designed to detect any deleterious effects of sandfish on shrimp, and vice versa. These experiments showed that a high stocking density of juvenile sandfish had no significant effects on growth and survival of shrimp. However, survival and...

  18. A white spot disease-like syndrome in the Pacific blue shrimp (Litopenaeus stylirostris) as a form of bacterial shell disease

    OpenAIRE

    Goarant, Cyrille; Brizard, Raphael; Marteau, Anne-laure

    2000-01-01

    In May 1997, some white lesions evoking the white spot syndrome disease were observed in Litopenaeus stylirostris broodstock in New Caledonia. The occurrence of these lesions was neither associated with mortality, nor with histological evidence of white spot syndrome baculovirus (WSBV), The evidence suggests that these lesions result from a form of bacterial disease and are associated with an increased bacterial flora on the outer surface of the cuticle, as well as an increased incidence thro...

  19. Ontogeny of osmoregulation in the Pacific blue shrimp, Litopenaeus stylirostris (Decapoda, Penaeidae): Deciphering the role of the Na+/K+-ATPase

    OpenAIRE

    Pham, Dominique; Charmantier, Guy; Boulo, Viviane; Wabete, Nelly; Ansquer, Dominique; Dauga, Clément; Grousset, Evelyse; Labreuche, Yannick; Charmantier-Daures, Mireille

    2016-01-01

    International audience The role of the main ion transporting enzyme Na +/K +-ATPase in osmoregulation processes was investigated in Litopenaeus stylirostris. The development and localization of the osmoregulation sites were studied during ontogenesis by immunodetection of Na+ K+-ATPase using monoclonal antibodies and transmission electron microscopy (TEM). Osmoregulation sites were identified as the pleurae and branchiostegites in the zoeae and mysis stages. In the subsequent post-metamorp...

  20. A Nonluminescent and Highly Virulent Vibrio harveyi Strain Is Associated with “Bacterial White Tail Disease” of Litopenaeus vannamei Shrimp

    OpenAIRE

    Junfang Zhou; Wenhong Fang; Xianle Yang; Shuai Zhou; Linlin Hu; Xincang Li; Xinyong Qi; Hang Su; Layue Xie

    2012-01-01

    Recurrent outbreaks of a disease in pond-cultured juvenile and subadult Litopenaeus vannamei shrimp in several districts in China remain an important problem in recent years. The disease was characterized by "white tail" and generally accompanied by mass mortalities. Based on data from the microscopical analyses, PCR detection and 16S rRNA sequencing, a new Vibrio harveyi strain (designated as strain HLB0905) was identified as the etiologic pathogen. The bacterial isolation and challenge test...

  1. Characterization of a primary cell culture from lymphoid organ of Litopenaeus vannamei and use for studies on WSSV replication

    OpenAIRE

    Li, W.; N. Thao; Corteel, M.; Dantas-Lima, J.J.; Thuong, K.V.; V. V. Tuan; Bossier, P.; Sorgeloos, P.; Nauwynck, H.

    2014-01-01

    Shrimp aquaculture is a booming agro-industry worldwide. Due to intensification of shrimp farming, pathogens emerge. Control of these pathogens especially viral pathogens is essential for a further expansion of this industry. Until now, the lack of shrimp cell lines has limited research on shrimp viral pathogens. In this context, a primary culture from the lymphoid organ of Litopenaeus vannamei was developed and standardized as a platform for further research on white spot syndrome virus (WSS...

  2. 凡纳对虾溶菌酶基因的克隆与性质研究%Gene Cloning and Characterization of Lysozyme Gene in Litopenaeus vannamei

    Institute of Scientific and Technical Information of China (English)

    杜志强; 王建英

    2013-01-01

      凡纳对虾先天免疫系统中的抗菌肽效应分子是发挥抗菌作用的关键分子,其中,溶菌酶分子在对抗细菌的侵染过程中,发挥着重要的清除细菌的作用。以凡纳对虾溶菌酶基因作为研究对象,通过进行基因克隆、氨基酸序列比对、系统进化关系分析以及分子结构的初步预测,推测该溶菌酶基因在凡纳对虾先天免疫系统中发挥着重要的抗菌作用。%  Antimicrobial peptide effectors played the key and important antibacterial role in the Litopenaeus vannamei innate immunity system. And the lysozyme also played an important part in the clearance of bacteria during the bacteria infection process. In this paper , Litopenaeus vannamei lysozyme gene was the research object. And the gene cloning, the amino acids sequence alignment, the phylogenetic relationship analysis, and the preliminary forecasts for molecular structure were done. We speculated that the lysozyme gene played an important part in the antibacterial function in the Litopenaeus vannamei innate immunity system.

  3. Caracterización preliminar de los invertebrados bentónicos capturados accidentalmente en la pesca de camarones en el norte del estado de Río de Janeiro, sudeste de Brasil Preliminary characterization of benthic invertebrates caught as by-catch in the shrimp fishery in the north of the Rio de Janeiro State, southeastern Brazil

    Directory of Open Access Journals (Sweden)

    Igor David da Costa

    2009-01-01

    Full Text Available Para caracterizar la biodiversidad de invertebrados bentónicos que componen la fauna asociada a la pesca de camarones en el puerto del Farol de Sao Thomé, costa norte del estado de Río de Janeiro, se realizaron 11 pescas mensuales en el año 2004 con redes de arrastre de fondo, cuya área de operaciones comprende 3-5 mn desde la línea de costa, entre 22°00'S y 22°20'S. Los datos registrados de cada taxon y/o especie se refieren a la frecuencia de ocurrencia, frecuencia numérica, biomasa, índice de Importancia Relativa y abundancia. En total se registraron 27 especies de invertebrados bentónicos de Porifera, Cnidaria, Mollusca, Annelida, Crustácea, Echinodermata y Bryozoa. Crustácea fue el más representativo, tanto en número de ejemplares de Petrochirus diogenes, Hepatus pudibundus y Callinectes ornatos, como en biomasa de P. diogenes y H. pudibundas. En términos de frecuencia de ocurrencia en los muéstreos, 11 especies (40,7% fueron constantes; 6 (22,2% accesorias y 10 (37,0% accidentales.In order to characterize the biodiversity of the benthic invertebrate by-catch associated with the shrimp fishery at Farol de Sao Thome harbor, northern Rio de Janeiro State, Brazil, in 2004, 11 monthly trawls were conducted using bottom trawl nets between 22°00'S and 22°20'S and from 3 to 5 nm from the shoreline. The analyzed data for each talon and/or species include frequency of occurrence, numeric frequency, biomass, index of Relative Importance, and abundance. In total, 27 benthic invertebrate species were recorded, including Peripheral, Cnidarians, Mollusk, Annelid, Crustacea, Echinodermata, and Bryozoa. The most representative group was Crustacea, both in number of specimens (Petrochirus diogenes, Hepatus pudibundus, Callinectes ornatus and in biomass (P. diogenes, H. pudibundus. In terms of the frequency of occurrence in the samples, 11 species (40.7% were constant, 6 species (22.2% were accessories, and 10 species (37.0% were by-catch.

  4. Técnica de digestibilidad in vitro en ingredientes y alimentos para camarón

    OpenAIRE

    NIETO LO?PEZ, MARTHA GUADALUPE; CRUZ SUA?REZ, LUCI?A ELIZABETH; RICQUE MARIE, DENIS; EZQUERRA BRAUER, MARINA

    2005-01-01

    En el presente trabajo se evaluo? si algunas te?cnicas in vitro o la digestibilidad en otras especies podri?an ser aplicadas en el control de calidad de harinas de pescado, dietas y otros ingredientes utilizados en la alimentacio?n del camaro?n Litopenaeus vanname. Para ello se determinaron los coeficientes de correlacio?n obtenidos en la digestibilidad in vivo en camaro?n y la digestibilidad in vivo en otras especies (salmo?n, trucha o mink), o con cada una de las diferentes te?cnicas in vit...

  5. Management of the fox (Vulpes vulpes) in Lombardy Region (Northern Italy) in relation to rabies / Interventi sulle popolazioni di Volpe (Vulpes vulpes) in Lombardia in relazione al controllo della rabbia silvestre

    OpenAIRE

    Guido Gerletti; Franca Guidali; Giovanni Scherini; Guido Tosi

    1991-01-01

    Abstract A description of the sanitary measures adopted in Lombardy Region against the spreading of rabies in populations of foxes is provided. Detailed accounts are reported for the Province of Sondrio. Riassunto Viene fornito un quadro sintetico relativo all'andamento dell'epidemia di rabbia silvestre nel territorio della Regione Lombardia e ai provvedimenti di controllo sanitario adottati nei confronti delle popolazioni di Volpe....

  6. Ataque de populações humanas por triatomíneos silvestres no Amazonas: uma nova forma de transmissão da infecção chagásica?

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    José Rodrigues Coura

    1994-12-01

    Full Text Available Comprovou-se o "ataque" de triatomineos silvestres (Rhodnius brethesi a colhedores de piaçava (Leopoldinia piaçaba, em piaçabal na localidade de Acuquaia, no Rio Padauari, afluente do Rio Negro, no Município de Barcelos, Estado do Amazonas, onde em trabalho anterior verificamos uma prevalência sorológica para anticorpos anti-T. cruzi em 12,5% da população na sede do município, com uma forte associação do contato dessa população com triatomineos silvestres, conhecidos na área como "piolho da piaçava", os quais vivem predominantemente nas piaçabeiras. O "ataque " de triatomineos a populações humanas foi possivelmente o mecanismo inicial de abordagem dos triatomineos ao homem, adaptando-se posteriormente ao seu peridomicílio e domicílio, principalmente em áreas desmaiadas ou de cerrado com poucos reservatórios silvestres. No caso do Amazonas, relatado neste trabalho, os triatomineos estavam provavelmente famintos por escassez de outros animais de sangue quente para sua alimentação.

  7. Uso do metabissulfito de sódio no controle de microorganismos em camarões marinhos Litopenaeus vannamei (Boone, 1931 = Use of sodium metabisulfite to control microorganism’s population in shrimps Litopenaeus vannamei (Boone, 1931

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    Lílian Maria Nery de Barros Góes

    2006-04-01

    Full Text Available O camarão cultivado destaca-se como um dos principais itens responsáveis pelo superávit da balança comercial do pescado brasileiro. Seu escoamento tem sido realizado para a América do Norte e Europa, onde as exigências de qualidade, especialmente quanto àcarga microbiana no camarão, são extremamente altas. Dadas as exigências dos importadores, objetivou-se avaliar a eficácia do conservante metabissulfito de sódio como agente antimicrobiano na carcinicultura. Camarões Litopenaeus vannamei de classificação 81/100 foram submetidos a 10 concentrações do metabissulfito de sódio (de 1% a 10%, durante 10 e 20 minutos. Foram realizadas contagens de bactérias aeróbias mesófilas, sendo as colônias isoladas submetidas ao teste bacterioscópico pelo método de Gram e a provas bioquímicas. Foram isoladas sete espécies de víbrio e observou-se que o número deunidades formadoras de colônias decresceu em função do uso de metabissulfito, ficando demonstrada a sua ação inibitória sobre as bactérias mesófilas aeróbias em todas as concentrações utilizadas.The cultivated shrimp comes if highlighting as one of the main responsible items for the superavit of the Brazilian’s fish trade balance. Its drainage has been accomplished for North America and Europe, where the quality demands is extremely high, especially for microbial load existent at shrimp. Given the demands of importers, the aim was to evaluate the effectiveness of conservator sodium metabisulfite as an antimicrobial agent in the shrimp culture. Shrimps Litopenaeus vannamei classified as 81/100 (10 to 12 g were submitted to 10 concentrations of sodium metabisulfite (from 1 to 10%, during 10 and 20 minutes. The aerobic mesophilic bacteria were counted and the isolated colonies were submitted to a bacterioscopic test using the Gram method and biochemical tests. It was observed that the number of colony-former units decreased due to metabisulfite use, thus demonstrating

  8. QUALITY OF THE WATER IN SHRIMP FARM Litopenaeus vannamei WITH SYSTEM OF PARTIAL RECIRCULATION SYSTEM. QUALIDADE DA ÁGUA EM FAZENDA DE CAMARÃO MARINHO Litopenaeus vannamei COM SISTEMA DE RECIRCULAÇÃO PARCIAL.

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    Luiz Otávio Brito

    2008-12-01

    Full Text Available The present work aim to characterize the quality of the water affluent and effluent of a shrimp Litopenaeus vannamei farm , which operates with system of partial recirculation system. The samples were accomplished during the low water and high tide periods of the day. The analyzed variables were: dissolved oxygen, temperature, pH, salinity, ammonia, nitrate, nitrite and phosphate. They samples were seven points, distributed strategically in way to characterize the water from the entrance going by the inside of the to your exit farm. ANOVA and Tukey test (P<0,05 were used in statistical analysis. Was observed the pH and dissolved oxygen stayed presenting averages of 7.72 and 6.58mg.L-1, respectively. The final averages went from 1.40 mg.L-1 to ammonia, 0.76 mg.L-1 for nitrate, 0.08 mg.L-1 for nitrite and 1.63 mg.L-1 for the phosphate.

    KEY WORDS: Quality of the water, recirculation, shrimp, vannamei.
    O presente trabalho teve como objetivo caracterizar a qualidade da água afluente e efluente de uma fazenda de camarão marinho Litopenaeus vannamei, que opera com sistema de recirculação parcial de água. As coletas foram realizadas durante os períodos de baixamar e preamar, diurnas, nos dias de lua. Analisaram-se as seguintes variáveis: oxigênio dissolvido, temperatura, pH, salinidade, amônia, nitrato, nitrito e fosfato. Escolheram-se sete pontos de coleta, distribuídos estrategicamente, de modo a caracterizar a água desde a entrada, passando pelo interior da fazenda até sua saída. Para análise estatística, utilizaram-se análise de variância (ANOVA e teste de Tukey (P<0,05. Observou-se que o pH e o oxigênio dissolvido apresentaram uma média de 7,72 e 6,58mg.L-1, respectivamente. As médias finais foram de 1,40mg.L-1 para amônia, 0,76mg.L-1 para nitrato, 0,08mg.L-1 para nitrito e 1,63mg.L-1 para o fosfato.

    PALAVRAS-CHAVES: Camarão, vannamei, qualidade da água, recirculação

  9. Tratamiento de efluentes del cultivo de Litopenaeus vannamei mediante procesos de sedimentación, filtración y absorción Treatments of effluents from Litopenaeus vannamei shrimp cultures through sedimentation, filtration and absorption

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    Roberto Ramos

    2010-01-01

    Full Text Available La eficiencia de remoción de materia particulada y nutrientes disueltos de efluentes de estanques de reproductores de Litopenaeus vannamei fue evaluada a escala de laboratorio, empleando tratamientos de sedimentación, filtración por Crassostrea rhizophorae y absorción por Ulva fasciata. En cada tratamiento se empleó un tiempo de residencia hidráulica del efluente de seis horas. En la etapa de sedimentación se utilizaron estanques con 90 L del efluente. Para la etapa de filtración, se emplearon estanques con 20 L del efluente sobrenadante de la etapa de sedimentación. En la absorción se utilizaron estanques con 15 L del efluente sobrenadante de la etapa de filtración. Al final de cada etapa se colectaron muestras de 500 mL para los análisis de calidad de agua. Los resultados indicaron que en la sedimentación, la turbidez, clorofila-a y sólidos suspendidos totales presentaron una remoción de 93,8%, 94,5% y 65,9%, respectivamente. La filtración alcanzó eficiencias de 89,3% y 100% para bacterias totales y clorofila a, respectivamente. En la absorción, fosfato (PO4-3 , amonio (N-NH4- y nitrato (N-NO3 fueron reducidos en 53,6%, 49,6% y 70,2%, respectivamente, en relación a los valores del estanque control. Los tratamientos combinados de sedimentación, filtración y absorción, bacterias totales (95,1%, turbidez (97,1% sólidos suspendidos totales (81,3%, clorofila-a (99,1%, amonio (54,1%, nitrito (58,0%, nitrato (69,2% y fosfato (52,9%, presentaron una alta eficiencia de remoción, en relación a los valores del efluente bruto. Se concluye que la combinación de tratamientos mejora significativamente la calidad de los efluentes del cultivo del camarón Litopenaeus vannamei.

  10. Arbovírus Ilheus em aves silvestres (Sporophila caerulescens e Molothrus bonariensis Ilheus arbovirus in wild birds (Sporophila caerulescens and Molothrus bonariensis

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    Luiz Eloy Pereira

    2001-04-01

    Full Text Available OBJETIVO: Relatar o isolamento do vírus Ilheus no Estado de São Paulo e avaliar o seu impacto para a saúde pública. MÉTODOS: O isolamento de vírus foi realizado em camundongos albinos Swiss, a partir de sangue de aves silvestres, capturadas com redes de espera tipo mist net, armadas no nível do solo, no Parque Ecológico do Tietê, São Paulo. A identificação das cepas isoladas foi feita pelos testes de inibição da hemaglutinação, fixação de complemento e neutralização em camundongos. Amostras de plasma de aves e de mamíferos silvestres foram submetidas à pesquisa sorológica para detecção de anticorpos inibidores de hemaglutinação. RESULTADOS: Foram isoladas duas cepas do vírus Ilheus em sangue de aves das espécies Sporophila caerulescens e Molothrus bonariensis e detectados anticorpos em aves das espécies Columbina talpacoti, Geopelia cuneata, Molothrus bonariensis e Sicalis flaveola, em sagüis das espécies Callithrix jacchus e Callithrix penicillata e no quati Nasua nasua. CONCLUSÕES: O isolamento do vírus Ilheus e a detecção de anticorpos específicos em aves residentes, migratórias e de cativeiro, em sagüis e quatis, comprovam a presença desse agente no Parque Ecológico do Tietê. O comportamento migratório de aves silvestres pode determinar a introdução do vírus em outras regiões. Considerando-se a patogenicidade para o homem e a confirmação da circulação desse agente viral em área urbana, freqüentada para atividade de lazer e de educação, o risco de ocorrência de infecção na população humana não pode ser descartado.OBJECTIVE: To report the first Ilheus arboviruses isolated from wild birds and analyze its public health impact. METHODS: Wild birds and mammals were captured using mist nets and Tomahawk traps, respectively. Blood samples were drawn from these animals and inoculated intracerebrally in Swiss suckling mice found in the Parque Ecológico do Tietê, Brazil. The isolates were

  11. Diarrea Viral Bovina (DVB y su Impacto en Poblaciones Silvestres: reporte de exposición en venado coliblanca (Odocoileus virginianus -resumen-

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    Pedro E Navas-Suárez

    2014-12-01

    Full Text Available Diversos eventos de origen antrópico (ej., incremento de la demanda alimenticia conducen a efectos como la ampliación de la frontera agrícola y las consecuentes interacciones entre poblaciones domésticas-productivas y silvestres‚ una de las cuales es el contacto directo que desde la perspectiva epidemiológica es riesgoso en términos de interfaz de agentes infecciosos en poblaciones; con lo cual‚ se hace necesario identificar y describir la ecología del patógeno para comprender su importancia en metapoblaciones (domésticos‚ productivos‚ silvestres y humanos. A nivel viral‚ los Pestivirus son conocidos por los impactos productivos y reproductivos en ungulados domésticos‚ mientras que en silvestres se han reportado eventos de morbi-mortalidad en poblaciones in situ de Bison bison‚ Odocoileus hemionus‚ O. virginianus y cabras salvajes confirmados por serología y aislamiento viral principalmente Diarrea Viral Bovina (DVB. En Colombia‚ DVB no es sujeta a control oficial‚ aunque según la Federación Colombiana de Ganaderos (FEDEGAN‚ es de importancia en ganaderías de leche y carne‚ con prevalencia histórica de 41% (2005-2009 (n=5219; por otro lado‚ ocupa el cuarto puesto en solicitud diagnóstica (1-leptospirosis‚ 2-rinotraqueitis infecciosa bovina‚ 3-leucosis‚ los departamentos de mayor diagnóstico son Antioquia‚ Caquetá y Cundinamarca; la enfermedad genera problemas reproductivos en ganaderías‚ donde indicadores reproductivos como el porcentaje de preñez‚ incremento de abortos o momias y disminución de la tasa de natalidad son afectados sensiblemente; al estar incluida en el complejo reproductivo bovino se debe monitorear y controlar. Por otro lado‚ en especies silvestres de Colombia no se conocen reportes de aislamiento o actividad inmunológica; aunque‚ mundialmente se considera que O. virginianus puede hospedarlo y generar un cuadro clínico similar al observado en bovinos (enfermedad o

  12. Vigilancia Epidemiológica de Rickettsia‚ Ehrlichia y Anaplasma en Fauna Silvestre ex situ e in situ de algunas Regiones de Colombia -resumen-

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    Santiago Monsalve-Buriticá

    2014-12-01

    Full Text Available Las enfermedades causadas por bacterias del orden de los Rickettsiales son trasmitidas por ectoparásitos originados en vida silvestre‚ sin embargo‚ en Colombia no existe una línea base del conocimiento en este sentido. Estos microorganismos intracelulares son causantes de enfermedades transmisibles en pacientes humanos; convirtiéndolas en zoonosis de tipo emergente. Este estudio ha permitido determinar la presencia de microorganismos del orden de los Rickettsiales (Ehrlichia spp.‚ Anaplasma spp.‚ y Rickettsia spp. en ejemplares silvestres de centros de conservación ex situ (Zoológicos‚ CAV y CAVR y en algunas regiones en condiciones in situ del país. De igual manera se identifica la infección de estos microorganismos en ectoparásitos vectores relacionados. Por medio de la técnica PCRrt‚ se detectó la presencia de Rickettsia spp.‚ Anaplasma spp.‚ y Ehrlichia spp.‚ en suero obtenido de mamíferos‚ y garrapatas colectadas de reptiles y mamíferos del Centro de Atención y Valoración de Fauna silvestre de la Corporación Autónoma Regional de los Valles del Sinú y San Jorge CAV-CVS en Montería (Córdoba‚ en osos hormigueros (Myrmecophaga tridactyla en Pore (Casanare‚ y en garrapatas obtenidas en ejemplares de titi gris (Saguinus leucopus y su hábitat en la vereda La Parroquia en Mariquita (Tolima. En Montería‚ los resultados obtenidos en mamíferos demostraron la ausencia de Rickettsia spp. en 72 muestras de suero‚ sin embargo se detectó un pool de garrapatas de la especie Amblyomma ovale positivo a este microorganismo‚ siendo el primer reporte en Colombia de ectoparásitos de Puma concolor positivos a Rickettsia spp.‚ mientras que en reptiles se detectaron 18 pools de garrapatas positivos a Rickettsia spp (90 %. En las garrapatas colectadas de nueve osos palmeros (Myrmecophaga tridactyla de vida libre se detectaron 12 pool de garrapatas positivos a este microorganismo (17‚6 %‚ y en ejemplares de

  13. Antioxidant Capacity, Anthocyanins, and Total Phenols of Wild and Cultivated Berries in Chile Capacidad Antioxidante, Antocianinas y Fenoles Totales de Berries Silvestres y Cultivados en Chile

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    Jaime Guerrero C

    2010-12-01

    ón fue determinar la capacidad antioxidante (AC, antocianinas totales (TA y el contenido de fenoles totales (TP, de berries silvestres y cultivados en diferentes localidades de la Región de La Araucanía y Región de Los Ríos (Chile. Para evaluar estos parámetros se utilizó el método de radicales libres 2,2-difenil-2- picrilhidrazilo (DPPH, pH diferencial y Folin-Ciocalteu, respectivamente. Los porcentajes de decoloración del DPPH estuvieron comprendidos entre 67,8% para zarzaparrilla roja y 95,3% para rosa mosqueta. El maqui presentó un contenido de AT significativamente mayor (2240,2 y 1445,3 mg L-1 cianidina 3-glucósido que el resto de los berries, el promedio de AT fue de 335,5 mg L-1. En cuanto al contenido FT los mayores niveles se obtuvieron en los dos cultivares de saskatoon (773,9 y 1001,9 mg L-1 ácido gálico y en rosa mosqueta silvestre (1457,0 y 1140,4 mg L-1 de ácido gálico. En este estudio se concluye que existen diferencias significativas en la capacidad antioxidante de frutos silvestres y cultivados en Chile, observándose una fuerte correlación entre el CA y el contenido de FT.

  14. Ecología de enfermedades infecciosas emergentes y conservación de especies silvestres Ecology of emerging infectious diseases and wild species conservation

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    G Medina-Vogel

    2010-01-01

    Full Text Available Las recientes publicaciones científicas respecto de enfermedades infecciosas emergentes en especies silvestres están aumentando la preocupación internacional en el deterioro de la salud del ecosistema de los océanos, ambientes terrestres y límnicos. Se estudiaron investigaciones publicadas en los últimos 25 años. Los objetivos de este estudio fueron: 1 determinar la importancia del hábitat y la introducción de especies alóctonas, dentro del contexto de la contaminación y el cambio climático, en el surgimiento de enfermedades infecciosas, y 2 evaluar el riesgo de extinción de especies con problemas de conservación. La evaluación de los casos escogidos, más importantes, permite concluir que los patógenos responsables de las enfermedades infecciosas en especies silvestres pueden transmitirse rápidamente entre huéspedes pudiendo llegar a producir en corto tiempo epizootias que pueden poner en peligro poblaciones vulnerables en declinación. La destrucción del hábitat y la introducción de especies alóctonas son variables ambientales que significativamente afectan la ecología de las enfermedades infecciosas emergentes. En cambio la contaminación y el cambio climático actúan como variables ambientales que favorecen la transmisión y la creación de nuevos huéspedes. Esta multicausalidad en el origen de las enfermedades genera la necesidad de un enfoque transdisciplinario para enfrentarlas. El riesgo creciente de epizootias debido a las variables ambientales y biológicas estudiadas, fuerza incluir en los planes de conservación y rescate de especies silvestres la ecología de las enfermedades infecciosas.Recent literature on emerging infectious diseases in wild species raises international concerns about ocean, terrestrial and freshwater ecosystem health. Reports documenting infectious diseases in wild species from the last 25 years were investigated. The goals of the study were to: 1 assess the importance of habitat and

  15. Efecto de ensilados de pescado e hígado de tiburón en el crecimiento de Litopenaeus schmitti, en sustitución de la harina y el aceite de pescado(Effect of fish silage and liver of sharks in the growth of Litopenaeus schmitti in place of fish meal and fish oil

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    Fraga-Castro, Iliana E

    2011-11-01

    Full Text Available ResumenDos diseños experimentales completamente aleatorizado se desarrollaron durante 6 semanas, para evaluar el efecto de dietas con ensilados de pescado (EP e hígado de tiburón (EHT, en el crecimiento de juveniles de camarón blanco Litopenaeus schmitti (peso inicial 1.3 ± 0.12 g. El EP con desechos de tilapia se incluyó en las dietas a niveles de 0, 16.5, 27.5 y 31 %, en sustitución de la harina de pescado.AbstractIn order to evaluate the effect of diets with fish silage (EP and shark liver (EHT, the growth of juvenile white shrimp Litopenaeus schmitti (mean initial weight 1.3 ± 0.12, developed a laboratory scale two completely randomized design for 6 weeks.

  16. Effect of copper on the growth of shrimps Litopenaeus vannamei: water parameters and copper budget in a recirculating system

    Science.gov (United States)

    Cheng, Bo; Liu, Ying; Yang, Hongsheng; Song, Yi; Li, Xian

    2014-09-01

    Shrimps ( Litopenaeus vannamei) were intensively cultured in a recirculating aquaculture system for 98 days to investigate effects of 0.3 mg/L Cu on its performance, Cu budget, and Cu distribution. Shrimps in Cu-treated systems had greater mean final weight (11.10 vs 10.50 g), body length (107.23 vs 106.42 mm), survival rate (67.80% vs 66.40%), and yield (6.42 vs 5.99 kg/m3), and lower feed conversion ratio (1.20 vs 1.29) than those in control systems but the differences were not significant. Vibrio numbers remained stable (104-106 colony forming units/mL) in the rearing tanks of both control and treated systems. Total ammonium-N, nitrite-N, nitrate-N, pH, chemical oxygen demand, 5-day carbonaceous biochemical oxygen demand, and total suspended solids were similar in controls and treatments. Dissolved Cu concentration in the treated systems decreased from 0.284 to 0.089 mg/L while in the control systems it increased from 0.006 2 to 0.018 mg/L. The main sources of Cu in the treated systems were the artificially added component (75.7% of total input), shrimp feed (21.0%), water (2.06%), and shrimp biomass (1.22%). The major outputs of Cu occurred via the mechanical filter (41.7%), water renewal (15.6%), and draining of the sediment trap (15.1%). The foam fractionator removed only 0.69% of total Cu input. Harvested shrimp biomass accounted for 11.68% of Cu input. The Cu concentration of shrimps in the Cu-treated systems (30.70 mg/kg wet weight) was significantly higher than that in control systems (22.02 mg/kg). Both were below the maximum permissible concentration (50 mg/kg) for Cu in seafood for human consumption in China. Therefore, recirculating systems can be used for commercial on-growing of Litopenaeus vannamei without loss of shrimp quality, even in water polluted by 0.30 mg/L Cu. The mechanical filter is the main route for Cu removal.

  17. Prevalência da infecção pelo Toxoplasma gondii em animais domésticos, silvestres e grupamentos humanos da Amazônia

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    José João Ferraroni

    1980-01-01

    Full Text Available Através de Reação de Hemaglutinação Indireta para toxoplasmose foram examinadas amostras de sangue de dez diferentes espécies de animais domésticos e silvestres, de um grupamento humano da cidade de Manaus-Amazonas e de um grupamento humano indígena de área distante, no território de Roraima. Em 108 animais domésticos, o exame sorológico foi reagente em 90,6% dos gatos (Felis catus, 68,4% dos cães (Canis familiaris, 60,0% dos bovinos (Bos sp, 41,2% dos galináceos (Gallus sp e 40,0% dos palmípedes (Cairina sp. Nos 104 animais silvestres foram reagentes 75,0% dos felídeos (Felis sp, 63,6% dos marsupiais (Didelphis marsupialis e Marmosa sp, 63,3% dos primatas (Saimiri sp e 61,1% dos roedores (Proechimys. Entre os dois grupos humanos a prevalência foi de 70,6% nos 51 habitantes da área de Manaus, 64,8% nos 37 silvícolas de Roraima. Os autores discutem os resultados obtidos, assim como os diversos aspectos envolvidos na epidemiologia da toxoplasmose e chamam a atenção para a existência de mecanismos de transmissão ainda não esclarecidos, enfatizando a necessidade de maiores estudos dessa zoonose.Serological examination for Toxoplasma gondii in human blood samples and in blood samples from ten different species of animals obtained in Manaus, State of Amazonas-Brazil, are compared with results obtained from similar examinations of blood from humans living in other areas of the Amazon basin. The domestic cat (Felis catus showed the highest levels of antibody for Toxoplasma gondii, whereas the domestic chicken (Gallus domesticus and duck (Cairina sp the lowest. The other animals showed similar intermediate levels of antibody to this protozoa. The authors discuss the results and several aspects of the involvement in epidemiology of toxoplasmosis and call attention to some transmission mechanisms not yet elucidated.

  18. High prevalence of Enterocytozoon hepatopenaei in shrimps Penaeus monodon and Litopenaeus vannamei sampled from slow growth ponds in India.

    Science.gov (United States)

    Biju, Narayanan; Sathiyaraj, Ganesan; Raj, Mithun; Shanmugam, Venu; Baskaran, Babu; Govindan, Umamaheswari; Kumaresan, Gayathri; Kasthuriraju, Karthick Kannan; Chellamma, Thampi Sam Raj Yohannan

    2016-08-01

    Hepatopancreatic microsporidiosis in cultivated Litopenaeus vannamei and Penaeus monodon is caused by the newly emerged pathogen Enterocytozoon hepatopenaei (EHP). It has been detected in shrimp cultured in China, Vietnam and Thailand and is suspected to have occurred in Malaysia and Indonesia and to be associated with severely retarded growth. Due to retarded shrimp growth being reported at farms in the major grow-out states of Tamilnadu, Andhra Pradesh and Odisha in India, shrimp were sampled from a total of 235 affected ponds between March 2014 and April 2015 to identify the presence of EHP. PCR and histology detected a high prevalence of EHP in both P. monodon and L. vannamei, and infection was confirmed by in situ hybridization using an EHP-specific DNA probe. Histology revealed basophilic inclusions in hepatopancreas tubule epithelial cells in which EHP was observed at various developmental stages ranging from plasmodia to mature spores. The sequence of a region of the small subunit rDNA gene amplified by PCR was found to be identical to EHP sequences deposited in GenBank. Bioassays confirmed that EHP infection could be transmitted orally to healthy shrimp. Histology also identified bacterial co-infections in EHP-infected shrimp sampled from slow-growth ponds with low-level mortality. The data confirm that hepatopancreatic microsporidiosis caused by EHP is prevalent in shrimp being cultivated in India. EHP infection control measures thus need to be implemented urgently to limit impacts of slowed shrimp growth. PMID:27503918

  19. Envelope Proteins of White Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT).

    Science.gov (United States)

    Xie, Shijun; Zhang, Xiaojun; Zhang, Jiquan; Li, Fuhua; Xiang, Jianhai

    2015-01-01

    White spot syndrome virus (WSSV) is a major pathogen in shrimp cultures. The interactions between viral proteins and their receptors on the surface of cells in a frontier target tissue are crucial for triggering an infection. In this study, a yeast two-hybrid (Y2H) library was constructed using cDNA obtained from the stomach and gut of Litopenaeus vannamei, to ascertain the role of envelope proteins in WSSV infection. For this purpose, VP37 was used as the bait in the Y2H library screening. Forty positive clones were detected after screening. The positive clones were analyzed and discriminated, and two clones belonging to the peritrophin family were subsequently confirmed as genuine positive clones. Sequence analysis revealed that both clones could be considered as the same gene, LV-peritrophin (LvPT). Co-immunoprecipitation confirmed the interaction between LvPT and VP37. Further studies in the Y2H system revealed that LvPT could also interact with other WSSV envelope proteins such as VP32, VP38A, VP39B, and VP41A. The distribution of LvPT in tissues revealed that LvPT was mainly expressed in the stomach than in other tissues. In addition, LvPT was found to be a secretory protein, and its chitin-binding ability was also confirmed.

  20. Envelope Proteins of White Spot Syndrome Virus (WSSV Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT.

    Directory of Open Access Journals (Sweden)

    Shijun Xie

    Full Text Available White spot syndrome virus (WSSV is a major pathogen in shrimp cultures. The interactions between viral proteins and their receptors on the surface of cells in a frontier target tissue are crucial for triggering an infection. In this study, a yeast two-hybrid (Y2H library was constructed using cDNA obtained from the stomach and gut of Litopenaeus vannamei, to ascertain the role of envelope proteins in WSSV infection. For this purpose, VP37 was used as the bait in the Y2H library screening. Forty positive clones were detected after screening. The positive clones were analyzed and discriminated, and two clones belonging to the peritrophin family were subsequently confirmed as genuine positive clones. Sequence analysis revealed that both clones could be considered as the same gene, LV-peritrophin (LvPT. Co-immunoprecipitation confirmed the interaction between LvPT and VP37. Further studies in the Y2H system revealed that LvPT could also interact with other WSSV envelope proteins such as VP32, VP38A, VP39B, and VP41A. The distribution of LvPT in tissues revealed that LvPT was mainly expressed in the stomach than in other tissues. In addition, LvPT was found to be a secretory protein, and its chitin-binding ability was also confirmed.

  1. Application of Wet Waste from Shrimp (Litopenaeus vannamei) with or Without Sea Mud to Feeding Sea Cucumber (Stichopus monotuberculatus)

    Institute of Scientific and Technical Information of China (English)

    CHEN Yanfeng; HU Chaoqun; RENChunhua

    2015-01-01

    In the present study, the applicability of the wet waste collected from shrimp (Litopenaeus vannamei) to the culture of sea cucumber (Stichopus monotuberculatus) was determined. The effects of dietary wet shrimp waste on the survival, specific growth rate (SGR), fecal production rate (FPR), ammonia- and nitrite-nitrogen productions of sea cucumber were studied. The total organic matter (TOM) level in the feces of sea cucumber was compared with that in corresponding feeds. Diet C (50% wet shrimp waste and 50% sea mud mash) made sea cucumber grow faster than other diets. Sea cucumber fed with either diet D (25% wet shrimp waste and 75% sea mud mash) or sole sea mud exhibited negative growth. The average lowest total FPR of sea cucumber occurred in diet A (wet shrimp waste), and there was no significant difference in total FPR between diet C and diet E (sea mud mash) (P>0.05). The average ammonia-nitrogen production of sea cucumber in different diet treatments decreased gradually with the decrease of crude protein content in different diets. The average highest nitrite-nitrogen production occurred in diet E treatment, and there was no sig-nificant difference in nitrite-nitrogen production among diet A, diet B (75% wet shrimp waste and 25% sea mud mash) and diet C treatments (P>0.05). In each diet treatment, the total organic matter (TOM) level in feces decreased to different extent compared with that in corresponding feeds.

  2. White Shrimp Litopenaeus vannamei That Have Received Gracilaria tenuistipitata Extract Show Early Recovery of Immune Parameters after Ammonia Stressing.

    Science.gov (United States)

    Chen, Yu-Yuan; Chen, Jiann-Chu; Lin, Yong-Chin; Yeh, Su-Tuen; Huang, Chien-Lun

    2015-06-05

    White shrimp Litopenaeus vannamei immersed in seawater (35‰) containing Gracilaria tenuistipitata extract (GTE) at 0 (control), 400, and 600 mg/L for 3 h were exposed to 5 mg/L ammonia-N (ammonia as nitrogen), and immune parameters including hyaline cells (HCs), granular cells (GCs, including semi-granular cells), total hemocyte count (THC), phenoloxidase (PO) activity, respiratory bursts (RBs), superoxide dismutase (SOD) activity, lysozyme activity, and hemolymph protein level were examined 24~120 h post-stress. The immune parameters of shrimp immersed in 600 mg/L GTE returned to original values earlier, at 96~120 h post-stress, whereas in control shrimp they did not. In another experiment, shrimp were immersed in seawater containing GTE at 0 and 600 mg/L for 3 h and examined for transcript levels of immune-related genes at 24 h post-stress. Transcript levels of lipopolysaccharide and β-1,3-glucan binding protein (LGBP), peroxinectin (PX), cytMnSOD, mtMnSOD, and HSP70 were up-regulated at 24 h post-stress in GTE receiving shrimp. We concluded that white shrimp immersed in seawater containing GTE exhibited a capability for maintaining homeostasis by regulating cellular and humoral immunity against ammonia stress as evidenced by up-regulated gene expression and earlier recovery of immune parameters.

  3. White Shrimp Litopenaeus vannamei That Have Received Gracilaria tenuistipitata Extract Show Early Recovery of Immune Parameters after Ammonia Stressing

    Directory of Open Access Journals (Sweden)

    Yu-Yuan Chen

    2015-06-01

    Full Text Available White shrimp Litopenaeus vannamei immersed in seawater (35‰ containing Gracilaria tenuistipitata extract (GTE at 0 (control, 400, and 600 mg/L for 3 h were exposed to 5 mg/L ammonia-N (ammonia as nitrogen, and immune parameters including hyaline cells (HCs, granular cells (GCs, including semi-granular cells, total hemocyte count (THC, phenoloxidase (PO activity, respiratory bursts (RBs, superoxide dismutase (SOD activity, lysozyme activity, and hemolymph protein level were examined 24~120 h post-stress. The immune parameters of shrimp immersed in 600 mg/L GTE returned to original values earlier, at 96~120 h post-stress, whereas in control shrimp they did not. In another experiment, shrimp were immersed in seawater containing GTE at 0 and 600 mg/L for 3 h and examined for transcript levels of immune-related genes at 24 h post-stress. Transcript levels of lipopolysaccharide and β-1,3-glucan binding protein (LGBP, peroxinectin (PX, cytMnSOD, mtMnSOD, and HSP70 were up-regulated at 24 h post-stress in GTE receiving shrimp. We concluded that white shrimp immersed in seawater containing GTE exhibited a capability for maintaining homeostasis by regulating cellular and humoral immunity against ammonia stress as evidenced by up-regulated gene expression and earlier recovery of immune parameters.

  4. Effects of periodical salinity fluctuation on the growth, molting, energy homeostasis and molting-related gene expression of Litopenaeus vannamei

    Science.gov (United States)

    Zhang, Dan; Guo, Xiantao; Wang, Fang; Dong, Shuanglin

    2016-04-01

    To determine the response of Litopenaeus vannamei to periodical salinity fluctuation, a 30-day experiment was conducted in laboratory. In this experiment, two salinity fluctuation amplitudes of 4 (group S4) and 10 (group S10) were designed. The constant salinity of 30 (group S0) was used as the control. Levels of shrimp growth, molting frequency (MF), cellular energy status (ATP, ADP and AMP), as well as the expression of genes encoding molt-inhibiting hormone (MIH), crustacean hyperglycemic hormone (CHH), ecdysteroid-regulated protein (ERP), and energy-related AMP-activated protein kinase (AMPK) were determined. The results showed that periodical salinity fluctuation significantly influenced all indicators except MF which ranged from 13.3% in group S10 to15.4% in group S4. In comparison with shrimps cultured at the constant salinity of 30, those in group S4 showed a significant elevation in growth rate, food conversion efficiency, cellular energy status, ERP and MIH gene transcript abundance, and a significant reduction in CHH and AMPK transcript abundance (P < 0.05). However, salinity fluctuation of 10 only resulted in a significant variation in MIH and CHH gene expression when compared to the control (P < 0.05). According to our findings, L. vannamei may be highly capable of tolerating salinity fluctuation. When ambient salinity fluctuated at approx. 4, the increased MF and energy stores in organisms may aid to promoting shrimp growth.

  5. Quality changes during frozen storage of blue shrimp (Litopenaeus stylirostris with antioxidant, α-tocopherol, under different conditions

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    Adriana Zulema Valencia-Perez

    2015-06-01

    Full Text Available Fresh blue shrimp (Litopenaeus stylirostris muscle was stored with antioxidants under different conditions: ANTIOX 2%, packed in bilayer film of polyamide-low density polyethylene film (PA-LDPE with 2% α-tocopherol; ANTIOX 4%, packed in PA-LDPE film with 4% α-tocopherol; and ANTIOX-GLAZED, samples stored glazed with 2% α-tocopherol. Shrimps packed in PA-LDPE without α-tocopherol were used as CONTROL. All samples were stored at –20 °C for 120 days. As compared to the CONTROL, the shrimp stored with the antioxidant showed lower lipid oxidation (0.10-0.14 vs 1.58 mgMA/kg of muscle, lost less firmness and astaxanthin content. ANTIOX 2% and ANTIOX-GLAZED showed the lowest concentrations of formaldehyde (0.081-0.083 μM/g. There were no significant differences in color and sensory properties, but differences in the integrity of the muscle fibers were observed. The treatments with α-tocopherol maintained the shrimp muscle quality during frozen storage. However, no significant differences were found between these treatments.

  6. Predictive ability of genomic selection models for breeding value estimation on growth traits of Pacific white shrimp Litopenaeus vannamei

    Science.gov (United States)

    Wang, Quanchao; Yu, Yang; Li, Fuhua; Zhang, Xiaojun; Xiang, Jianhai

    2016-10-01

    Genomic selection (GS) can be used to accelerate genetic improvement by shortening the selection interval. The successful application of GS depends largely on the accuracy of the prediction of genomic estimated breeding value (GEBV). This study is a first attempt to understand the practicality of GS in Litopenaeus vannamei and aims to evaluate models for GS on growth traits. The performance of GS models in L. vannamei was evaluated in a population consisting of 205 individuals, which were genotyped for 6 359 single nucleotide polymorphism (SNP) markers by specific length amplified fragment sequencing (SLAF-seq) and phenotyped for body length and body weight. Three GS models (RR-BLUP, BayesA, and Bayesian LASSO) were used to obtain the GEBV, and their predictive ability was assessed by the reliability of the GEBV and the bias of the predicted phenotypes. The mean reliability of the GEBVs for body length and body weight predicted by the different models was 0.296 and 0.411, respectively. For each trait, the performances of the three models were very similar to each other with respect to predictability. The regression coefficients estimated by the three models were close to one, suggesting near to zero bias for the predictions. Therefore, when GS was applied in a L. vannamei population for the studied scenarios, all three models appeared practicable. Further analyses suggested that improved estimation of the genomic prediction could be realized by increasing the size of the training population as well as the density of SNPs.

  7. ZResponse to selection, heritability and genetic correlations between body weight and body size in Pacific white shrimp, Litopenaeus vannamei

    Science.gov (United States)

    Andriantahina, Farafidy; Liu, Xiaolin; Huang, Hao; Xiang, Jianhai

    2012-03-01

    To quantify the response to selection, heritability and genetic correlations between weight and size of Litopenaeus vannamei, the body weight (BW), total length (TL), body length (BL), first abdominal segment depth (FASD), third abdominal segment depth (TASD), first abdominal segment width (FASW), and partial carapace length (PCL) of 5-month-old parents and of offspnng were measured by calculating seven body measunngs of offspnng produced by a nested mating design. Seventeen half-sib families and 42 full-sib families of L. vannamei were produced using artificial fertilization from 2-4 dams by each sire, and measured at around five months post-metamorphosis. The results show that hentabilities among vanous traits were high: 0.515±0.030 for body weight and 0.394±0.030 for total length. After one generation of selection. the selection response was 10.70% for offspring growth. In the 5th month, the realized heritability for weight was 0.296 for the offspnng generation. Genetic correlations between body weight and body size were highly variable. The results indicate that external morphological parameters can be applied dunng breeder selection for enhancing the growth without sacrificing animals for determining the body size and breed ability; and selective breeding can be improved significantly, simultaneously with increased production.

  8. Hypoxia drives apoptosis independently of p53 and metallothionein transcript levels in hemocytes of the whiteleg shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Felix-Portillo, Monserrath; Martínez-Quintana, José A; Arenas-Padilla, Marina; Mata-Haro, Verónica; Gómez-Jiménez, Silvia; Yepiz-Plascencia, Gloria

    2016-10-01

    The cellular mechanisms used by the shrimp Litopenaeus vannamei to respond to hypoxia have been studied from the energetic metabolism and antioxidant angles. We herein investigated the participation of p53 and metallothionein (MT) in the apoptotic process in response to hypoxia in shrimp hemocytes. The Lvp53 or LvMT genes were efficiently silenced by injection of double stranded RNA for p53 or MT. The effects of silencing on apoptosis were measured as caspase-3 activity and flow cytometry in hemocytes after 24 and 48 h of hypoxia (1.5 mg DO L(-1)). Hemocytes from unsilenced animals had significantly higher apoptosis levels upon both times of hypoxia. The apoptotic levels were diminished but not suppressed in dsp53-silenced but not dsMT-silenced hemocytes after 24 h of hypoxia, indicating a contribution of Lvp53 to apoptosis. Apoptosis in normoxia was significantly higher in dsp53-and dsMT-silenced animals compared to the unsilenced controls, pointing to a possible cytoprotective role of LvMT and Lvp53 during the basal apoptotic program in normoxia. Overall, these results indicate that hypoxia augments apoptosis in shrimp hemocytes and high mRNA levels of Lvp53 and LvMT are not necessary for this response. PMID:27459156

  9. Dextrose as carbon source in the culture of Litopenaeus vannamei (Boone, 1931 in a zero exchange system

    Directory of Open Access Journals (Sweden)

    Sabrina M Suita

    2015-07-01

    Full Text Available This work compared the use of dextrose and molasses as carbon sources for biofloc development, water quality maintenance, microorganism composition and growth performance of Litopenaeus vannamei juveniles in biofloc technology (BFT. Two treatments, dextrose and molasses, were tested with four replicates each. Carbon was added to achieve a C:N-AT (N-(NH3+NH4+ ratio of 6:1. Physical and chemical water quality variables were monitored daily, and shrimp growth was estimated through periodic biometry. After 30 days, survival, final biomass, and feeding conversion rate (FCR were determined. Dissolved organic carbon, chlorophyll-a, floc volume, total ammonia, nitrite, nitrate and phosphate concentrations, and microorganisms (qualified by groups, were measured every three days. Water quality variables remained within acceptable levels throughout the experimental period, except for nitrite, which reached higher levels than recommended for this species. The use of dextrose resulted in higher water transparency, which influenced the remaining centric diatoms. A superior shrimp performance was observed at this treatment, presumably because of variations on the microbial community. Therefore, it is concluded that the addition of dextrose results in a superior growth performance of L. vannamei when cultured in BFT systems.

  10. RAPD markers as predictors of infectious hypodermal and hematopoietic necrosis virus (IHHNV) resistance in shrimp (Litopenaeus stylirostris).

    Science.gov (United States)

    Hizer, Suzanne E; Dhar, Arun K; Klimpel, Kurt R; Garcia, Denise K

    2002-02-01

    Random amplified polymorphic DNA (RAPD) fingerprints of two shrimp populations (Litopenaeus stylirostris) were compared to find genetic marker(s) that may be associated with infectious hypodermal and hematopoietic necrosis virus (IHHNV) resistance or susceptibility. Of the 100 10-mer random primers and 100 intersimple-sequence repeat (ISSR) primers screened, five provided markers specific to the Super Shrimp population and three provided markers specific to the wild caught population. The two populations were further characterized for relative viral load (reported as cycle threshold, CT) using real-time quantitative PCR with primers specific to the IHHNV genome. The beta-actin gene was amplified to serve as a control for normalization of the IHHNV viral load. The mean viral load was significantly lower (C(T) = 34.58; equivalent to 3.3 x 10(1) copies of IHHNV genome/ng DNA) in Super Shrimp than in the wild caught population (CT = 23.49; equivalent to 4.2 x 10(4) copies/ng DNA; P < 0.001; CT values are inversely related to viral load). A preliminary prediction model was created with Classification and Regression Tree (CART) software (Salford Systems, San Diego, Calif.), where the resultant decision tree uses the presence or absence of seven RAPD markers as predictors of the relative viral load. PMID:11908650

  11. Double-stranded RNA confers both preventive and therapeutic effects against Penaeus stylirostris densovirus (PstDNV) in Litopenaeus vannamei.

    Science.gov (United States)

    Ho, Teerapong; Yasri, Pratchayapong; Panyim, Sakol; Udomkit, Apinunt

    2011-01-01

    Penaeus stylirostris densovirus (PstDNV) infection is found widespread in peneaid shrimp, especially in economically important species such as black tiger shrimp Penaeus monodon and Pacific white shrimp Litopenaeus vannamei. Although effective prevention method for viral diseases is not well established in shrimp, the treatment with viral specific double-stranded RNA (dsRNA) or siRNA has given promising results. In present study, dsRNAs corresponding to non-structural (ORF1 and ORF2 overlapping sequence) and structural (ORF3) genes of PstDNV were investigated for their potency to inhibit PstDNV replication in the shrimp. Periodically injection of either ORF1-2 dsRNA or ORF3 dsRNA at three days interval into L. vannamei resulted in substantial inhibition of PstDNV infection. In addition, a possibility for a therapeutic application of dsRNA in PstDNV-infected shrimp was demonstrated by the efficient suppression of PstDNV replication in L. vannamei when the ORF1-2 dsRNA was delivered into the shrimp within 24h post-PstDNV injection. Hence, our results established both the preventive and therapeutic potency of dsRNA to inhibit PstDNV in L. vannamei that could be applied as a potential treatment of PstDNV infection in shrimp. PMID:20869997

  12. Protection of blue shrimp (Litopenaeus stylirostris) against the White Spot Syndrome Virus (WSSV) when injected with shrimp lysozyme.

    Science.gov (United States)

    Mai, Wei-jun; Wang, Wei-na

    2010-04-01

    In this study we found that a blue shrimp (Litopenaeus stylirostris) lysozyme gene (Lslzm) was up-regulated in WSSV-infected shrimp, suggesting that lysozyme is involved in the innate response of shrimp to this virus. Shrimp were intramuscularly injected with Lslzm protein to identify how this recombinant protein protects L. stylirostris from WSSV infection and to determine how this protein influences nonspecific cellular and humoral defense mechanisms. Higher survival rates and a lower viral load (compared with controls) were reported for shrimps that were first injected with the Lslzm protein and then infected with WSSV. In addition, the Lslzm expression level and the immunological parameters (including THC, phagocytic activity, respiratory burst activity, phenoloxidase activity and lysozyme activity) were all significantly higher in the WSSV-infected shrimp treated with the Lslzm protein, compared with the controls. These results indicate that lysozyme is effective at blocking WSSV infection in L. stylirostris and that lysozyme modulates the cellular and humoral defense mechanisms after they are suppressed by the WSSV virus. PMID:20074645

  13. Viral interference between infectious hypodermal and hematopoietic necrosis virus and white spot syndrome virus in Litopenaeus vannamei.

    Science.gov (United States)

    Bonnichon, Valérie; Lightner, Donald V; Bonami, Jean-Robert

    2006-10-17

    White spot syndrome virus (WSSV) is highly virulent and has caused significant production losses to the shrimp culture industry over the last decade. Infectious hypodermal and hematopoietic necrosis virus (IHHNV) also infects penaeid shrimp and, while being less important than WSSV, remains a major cause of significant production losses in Litopenaeus vannamei (also called Penaeus vannamei) and L. stylirostris (also called Penaeus stylirostris). These 2 viruses and their interactions were previously investigated in L. stylirostris. We report here laboratory challenge studies carried out to determine if viral interference between IHHNV and WSSV also occurs in L. vannamei, and it was found that experimental infection with IHHNV induced a significant delay in mortality following WSSV challenge. L. vannamei infected per os with IHHNV were challenged with WSSV at 0, 10, 20, 30, 40 and 50 d post-infection. Groups of naïve shrimp infected with WSSV alone died in 3 d whereas shrimp pre-infected with IHHNV for 30, 40 or 50 d died in 5 d. Real-time PCR analysis showed that the delay correlated to the IHHNV load and that WSSV challenge induced a decrease in IHHNV load, indicating some form of competition between the 2 viruses. PMID:17140141

  14. Metal and metalloid bioaccumulation in the Pacific blue shrimp Litopenaeus stylirostris (Stimpson) from New Caledonia: laboratory and field studies.

    Science.gov (United States)

    Metian, Marc; Hédouin, Laetitia; Eltayeb, Mohamed M; Lacoue-Labarthe, Thomas; Teyssié, Jean-Louis; Mugnier, Chantal; Bustamante, Paco; Warnau, Michel

    2010-01-01

    The present work aimed at better understanding metal and metalloid bioaccumulation in the edible Pacific blue shrimp Litopenaeus stylirostris, using both laboratory and field approaches. In the laboratory, the bioaccumulation kinetics of Ag, Cd, Co, Cr, and Zn have been investigated in shrimp exposed via seawater and food, using the corresponding gamma-emitting radiotracers ((110 m)Ag, (109)Cd, (57)Co, (51)Cr, and (65)Zn) and highly sensitive nuclear detection techniques. Results showed that hepatopancreas and intestine concentrated the metals to the highest extent among the blue shrimp organs and tissues. Moulting was found to play a non negligible detoxification role for Co, Cr and, to a lesser extent, Zn. Metal retention by L. stylirostris widely varied (from a few days to several months), according to the element and exposure pathway considered (a given metal was usually less strongly retained when ingested with food than when it was taken up from the dissolved phase). In the field study, Ag, As, Cd, Co, Cr, Cu, Mn, Ni, and Zn were analysed in shrimp collected from a New Caledonian aquaculture pond. Metal concentrations in the shrimp muscles were generally relatively low and results confirmed the role played by the digestive organs and tissues in the bioaccumulation/storage/detoxification of metals in the Pacific blue shrimp. Preliminary risk considerations indicate that consumption of the shrimp farmed in New Caledonia is not of particular concern for human health. PMID:20637480

  15. cDNA cloning, expression and antibacterial activity of lysozyme C in the blue shrimp (Litopenaeus stylirostris)

    Institute of Scientific and Technical Information of China (English)

    Weijun Mai; Chaoqun Hu

    2009-01-01

    The gene coding for lysozyme in blue shrimp (Litopenaeus stylirostris) was cloned, sequenced and expressed in pET-32a vector. The deduced amino acid sequence of F. Merguiensi lysozyme showed 37-93% similarity with the mouse, human, chicken, and tiger prawn counterparts. The lysozyme was purified to homogeneity and observed as a band of approximately 15 kDa in 15% SDS-PAGE. Semi-quantitative RT-PCR analysis demonstrated that mRNA transcripts of lysozyme could be mainly detected in the tissues of haemocytes, gill, gonad and the lymphoid organ of unchallenged shrimps, whereas the expression of lysozyme transcripts was increased in all the tested tissues after the heat-killed Vibrio alginolyticus challenge. The temporal expression of lysozyme mRNA in haemolymph challenged by Micrococcus luteus and V. Alginolyticus was both up-regulated and reached the maximum level at 8 and 16 h post-stimulation, respec-tively, and then dropped back to the original level. Bacteriolytic activity of the lysozyme against different bacterial cultures was deter-mined by the solid phase and turbidimetric assays. The results demonstrated that the lysozyme we obtained was not only against Gram-positive and Gram-negative bacteria but also against shrimp pathogens V. Alginolyticus and V. Parahemolyticus. In addition, the study of the inhibition mechanism revealed that the antibacterial activity of the lysozyme was a result of the bactericidal effect.

  16. Effects of periodical salinity fluctuation on the growth, molting, energy homeostasis and molting-related gene expression of Litopenaeus vannamei

    Science.gov (United States)

    Zhang, Dan; Guo, Xiantao; Wang, Fang; Dong, Shuanglin

    2016-10-01

    To determine the response of Litopenaeus vannamei to periodical salinity fluctuation, a 30-day experiment was conducted in laboratory. In this experiment, two salinity fluctuation amplitudes of 4 (group S4) and 10 (group S10) were designed. The constant salinity of 30 (group S0) was used as the control. Levels of shrimp growth, molting frequency (MF), cellular energy status (ATP, ADP and AMP), as well as the expression of genes encoding molt-inhibiting hormone (MIH), crustacean hyperglycemic hormone (CHH), ecdysteroid-regulated protein (ERP), and energy-related AMP-activated protein kinase (AMPK) were determined. The results showed that periodical salinity fluctuation significantly influenced all indicators except MF which ranged from 13.3% in group S10 to15.4% in group S4. In comparison with shrimps cultured at the constant salinity of 30, those in group S4 showed a significant elevation in growth rate, food conversion efficiency, cellular energy status, ERP and MIH gene transcript abundance, and a significant reduction in CHH and AMPK transcript abundance ( P < 0.05). However, salinity fluctuation of 10 only resulted in a significant variation in MIH and CHH gene expression when compared to the control ( P < 0.05). According to our findings, L. vannamei may be highly capable of tolerating salinity fluctuation. When ambient salinity fluctuated at approx. 4, the increased MF and energy stores in organisms may aid to promoting shrimp growth.

  17. Structure of nucleoside diphosphate kinase from pacific shrimp (Litopenaeus vannamei) in binary complexes with purine and pyrimidine nucleoside diphosphates.

    Science.gov (United States)

    López-Zavala, Alonso A; Quintero-Reyes, Idania E; Carrasco-Miranda, Jesús S; Stojanoff, Vivian; Weichsel, Andrzej; Rudiño-Piñera, Enrique; Sotelo-Mundo, Rogerio R

    2014-09-01

    Nucleoside diphosphate kinase (NDK; EC 2.7.4.6) is an enzyme that catalyzes the third phosphorylation of nucleoside diphosphates, leading to nucleoside triphosphates for DNA replication. Expression of the NDK from Litopenaeus vannamei (LvNDK) is known to be regulated under viral infection. Also, as determined by isothermal titration calorimetry, LvNDK binds both purine and pyrimidine deoxynucleoside diphosphates with high binding affinity for dGDP and dADP and with no heat of binding interaction for dCDP [Quintero-Reyes et al. (2012), J. Bioenerg. Biomembr. 44, 325-331]. In order to investigate the differences in selectivity, LvNDK was crystallized as binary complexes with both acceptor (dADP and dCDP) and donor (ADP) phosphate-group nucleoside diphosphate substrates and their structures were determined. The three structures with purine or pyrimidine nucleotide ligands are all hexameric. Also, the binding of deoxy or ribonucleotides is similar, as in the former a water molecule replaces the hydrogen bond made by Lys11 to the 2'-hydroxyl group of the ribose moiety. This allows Lys11 to maintain a catalytically favourable conformation independently of the kind of sugar found in the nucleotide. Because of this, shrimp NDK may phosphorylate nucleotide analogues to inhibit the viral infections that attack this organism.

  18. Effect of Salinity on the Biosynthesis of Amines in Litopenaeus vannamei and the Expression of Gill Related Ion Transporter Genes

    Institute of Scientific and Technical Information of China (English)

    PAN Luqing; LIU Hongyu; ZHAO Qun

    2014-01-01

    This study examined the effect of salinity on the expression of Na+/K+-ATPase (NKA) α-subunit and vacuolar-type H+-ATPase (V-ATPase) β-subunit gene in the gill of Litopenaeus vannamei. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay showed that the expression of NKAα-subunit and V-ATPaseβ-subunit gene was significantly influ-enced by salinity. It was found that the NKA activity significantly varied with salinity in time and dose dependent manner;whereas the V-ATPase activity did not. The abundance of NKAα-subunit gene transcript increased rapidly when the salinity decreased from 26b to 21, and slowly when the salinity decreased from 26 to 31 within the first 24 h. When the salinity decreased from 26 to 21, the transcription of NKAα-subunit gene in gill epithelium was higher at 12 h than that at 0 h, which was consistent with the result of immunoblotting assay of NKAα-subunit. In addition, salinity had a significant time-and dose-dependent effect on the concentration of biogenic amines in both hemolymph and gill. As compared to other parameters, the concentration of dopamine (DA) and 5-hydroxytryptamine (5-HT) varied in different patterns when the salinity decreased from 26 to 21 or increased from 26 to 31, sug-gesting that DA and 5-HT played different regulatory roles in osmotic adaption and modulation of shrimp when salinity varies.

  19. TAT improves in vitro transportation of fortilin through midgut and into hemocytes of white shrimp Litopenaeus vannamei

    Science.gov (United States)

    Zhou, Yi; Zhang, Wenbing; Mai, Kangsen; Xu, Wei; Zhang, Yanjiao; Ai, Qinghui; Wang, Xiaojie

    2012-06-01

    Fortilin is a multifunctional protein implicated in many important cellular processes. Since injection of Pm-fortilin reduces shrimp mortality caused by white spot syndrome virus (WSSV), there is potential application of fortilin in shrimp culture. In the present study, in order to improve trans-membrane transportation efficiency, the protein transduction domain of the transactivator of transcription (TAT) peptide was fused to fortilin. The Pichia pastoris yeast expression system, which is widely accepted in animal feeds, was used for production of recombinant fusion protein. Green fluorescence protein (GFP) was selected as a reporter because of its intrinsic visible fluorescence. The fortilin, TAT and GFP fusion protein were constructed. Their trans-membrane transportation efficiency and effects on immune response of shrimp were analyzed in vitro. Results showed that TAT peptide improved in vitro uptake of fortilin into the hemocytes and midgut of Litopenaeus vannamei. The phenoloxidase (PO) activity of hemocytes incubated with GFP-Fortilin or GFP-Fortilin-TAT was significantly increased compared with that in the control without expressed fortilin. The PO activity of hemocytes incubated with 200 μg mL-1 GFP-Fortilin-TAT was significantly higher than that in the group with the same concentration of GFP-Fortilin. Hemocytes incubated with GFP-Fortilin-TAT at all concentrations showed significantly higher nitric oxide synthase (NOS) activity than those in the control or in the GFP-Fortilin treatment. The present in vitro study indicated that TAT fusion protein improved the immune effect of fortilin.

  20. ZResponse to selection, heritability and genetic correlations between body weight and body size in Pacific white shrimp,Litopenaeus vannamei

    Institute of Scientific and Technical Information of China (English)

    Farafidy ANDRIANTAHINA; LIU Xiaolin; HUANG Hao; XIANG Jianhai

    2012-01-01

    To quantify the response to selection,heritability and genetic correlations between weight and size of Litopenaeus vannamei,the body weight (BW),total length (TL),body length (BL),first abdominal segment depth (FASD),third abdominal segment depth (TASD),first abdominal segment width (FASW),and partial carapace length (PCL) of 5-month-old parents and of offspring were measured by calculating seven body measurings of offspring produced by a nested mating design.Seventeen half-sib families and 42 full-sib families of L.vannamei were produced using artificial fertilization from 2-4 dams by each sire,and measured at around five months post-metamorphosis.The results show that heritabilities among various traits were high:0.515+0.030 for body weight and 0.394+0.030 for total length.After one generation of selection,the selection response was 10.70% for offspring growth.In the 5th month,the realized heritability for weight was 0.296 for the offspring generation.Genetic correlations between body weight and body size were highly variable.The results indicate that external morphological parameters can be applied during breeder selection for enhancing the growth without sacrificing animals for determining the body size and breed ability; and selective breeding can be improved significantly,simultaneously with increased production.

  1. Identification of Bacterial Community Composition in Freshwater Aquaculture System Farming of Litopenaeus vannamei Reveals Distinct Temperature-Driven Patterns

    Directory of Open Access Journals (Sweden)

    Yuyi Tang

    2014-08-01

    Full Text Available Change in temperature is often a major environmental factor in triggering waterborne disease outbreaks. Previous research has revealed temporal and spatial patterns of bacterial population in several aquatic ecosystems. To date, very little information is available on aquaculture environment. Here, we assessed environmental temperature effects on bacterial community composition in freshwater aquaculture system farming of Litopenaeus vannamei (FASFL. Water samples were collected over a one-year period, and aquatic bacteria were characterized by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE and 16S rDNA pyrosequencing. Resulting DGGE fingerprints revealed a specific and dynamic bacterial population structure with considerable variation over the seasonal change, suggesting that environmental temperature was a key driver of bacterial population in the FASFL. Pyrosequencing data further demonstrated substantial difference in bacterial community composition between the water at higher (WHT and at lower (WLT temperatures in the FASFL. Actinobacteria, Proteobacteria and Bacteroidetes were the highest abundant phyla in the FASFL, however, a large number of unclassified bacteria contributed the most to the observed variation in phylogenetic diversity. The WHT harbored remarkably higher diversity and richness in bacterial composition at genus and species levels when compared to the WLT. Some potential pathogenenic species were identified in both WHT and WLT, providing data in support of aquatic animal health management in the aquaculture industry.

  2. Evaluation of Pacific white shrimp (Litopenaeus vannamei health during a superintensive aquaculture growout using NMR-based metabolomics.

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    Tracey B Schock

    Full Text Available Success of the shrimp aquaculture industry requires technological advances that increase production and environmental sustainability. Indoor, superintensive, aquaculture systems are being developed that permit year-round production of farmed shrimp at high densities. These systems are intended to overcome problems of disease susceptibility and of water quality issues from waste products, by operating as essentially closed systems that promote beneficial microbial communities (biofloc. The resulting biofloc can assimilate and detoxify wastes, may provide nutrition for the farmed organisms resulting in improved growth, and may aid in reducing disease initiated from external sources. Nuclear magnetic resonance (NMR-based metabolomic techniques were used to assess shrimp health during a full growout cycle from the nursery phase through harvest in a minimal-exchange, superintensive, biofloc system. Aberrant shrimp metabolomes were detected from a spike in total ammonia nitrogen in the nursery, from a reduced feeding period that was a consequence of surface scum build-up in the raceway, and from the stocking transition from the nursery to the growout raceway. The biochemical changes in the shrimp that were induced by the stressors were essential for survival and included nitrogen detoxification and energy conservation mechanisms. Inosine and trehalose may be general biomarkers of stress in Litopenaeus vannamei. This study demonstrates one aspect of the practicality of using NMR-based metabolomics to enhance the aquaculture industry by providing physiological insight into common environmental stresses that may limit growth or better explain reduced survival and production.

  3. Effect of culture intensity and probiotics application on microbiological and environmental parameters in Litopenaeus vannamei culture ponds.

    Science.gov (United States)

    Patil, Prasanna Kumar; Muralidhar, M; Solanki, Haresh G; Patel, Pretesh P; Patel, Krishna; Gopla, Chavali

    2016-01-01

    The present study examined the complex interaction among stocking density and extent of probiotic use with production and environmental parameters in Litopenaeus vannamei culture ponds to suggest suitable management strategies. The study was conducted inL. vannamei culture ponds with stocking density of 35 nos sq m(-1) (Group I) and 56 nos sq m(-1) (Group II) and probiotic application @16.5 kg ha(-1) and 157 kg ha(-1), respectively. There was no significant difference noted between the two groups of ponds in respect to ammonia oxidizing bacteria (AOB) in sediment and nitrite oxidizing bacteria (NOB) in water samples, whereas significantly higher levels of AOB in water samples of high intensity culture ponds (Group II) and NOB in sediment samples of Group I were observed. The levels of sulphur oxidizing bacteria (SOB) and sulphur reducing bacteria (SRB) in Group I pond water and in Group II sediment were significantly higher than their corresponding levels in the other group. In both the groups, ammonia, nitrite and sulphide concentrations were below toxic limits prescribed for shrimp farming. Comparing the production parameters at harvest revealed that low intensity culture ponds (Group I) had higher growth rate, average body weight and significantly lower FCR and higher survival rate than high intensity culture ponds (Group II). The results indicated that application of microbial products in higher quantities did not benefit significantly, and there is a need to regulate quantum and schedule of biological product usage for economically sustainable shrimp culture. PMID:26930856

  4. Population genetic structure of Brazilian shrimp species (Farfantepenaeus sp., F. brasiliensis, F. paulensis and Litopenaeus schmitti: Decapoda: Penaeidae

    Directory of Open Access Journals (Sweden)

    Jaqueline Gusmão

    2005-03-01

    Full Text Available Penaeid shrimps are important resources for worldwide fisheries and aquaculture. In the Southwest Atlantic, Farfantepenaeus brasiliensis, F. paulensis, F. subtilis, Farfantepenaeus sp. and Litopenaeus schmitti are among the most important commercially exploited species. Despite their high commercial value, there is little information available on the different aspects of their biology or genetics and almost no data on their stock structure. We used allozymes to estimate variability levels and population genetic structure of F. brasiliensis, F. paulensis, L. schmitti and the recently detected species Farfantepenaeus sp. along as much as 4,000 km of Brazilian coastline. No population heterogeneity was detected in F. brasiliensis or L. schmitti along the studied area. In contrast, F ST values found for Farfantepenaeus sp. and F. paulensis indicate that the populations of those two species are genetically structured, comprising different fishery stocks. The largest genetic differences in F. paulensis were found between Lagoa dos Patos (South and the two populations from Southeast Brazil. In Farfantepenaeus sp., significant differences were detected between the population from Recife and those from Fortaleza and Ilhéus.

  5. Efecto del fotoperiodo y la temperatura sobre la composición bioquímica en reproductores silvestres de cabrilla sardinera, Mycteroperca rosacea (Streets, 1877

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    José Antonio Estrada-Godínez

    2014-03-01

    Full Text Available Se estimó el factor de condición (K, el índice gonadosomático (IGS, hepatosomático (IHS y de grasa visceral (IGV, así como la composición bioquímica en diferentes tejidos de reproductores silvestres de cabrilla sardinera, Mycteroperca rosacea, para evaluarlos a lo largo de un ciclo reproductivo y ver la relación que presentan los cambios con respecto a la temperatura del agua y fotoperiodo de la zona de muestreo. Se capturaron 187 reproductores, 146 hembras y 41 machos en el golfo de California, México. Se observaron diferencias significativas (P < 0,005 en los IGS, IHS e IGV; en el caso de K no se encontraron diferencias. También, se observaron variaciones significativas (P < 0,05 en la mayoría de los parámetros bioquímicos, encontrándose los valores más altos durante la etapa de desove, mientras que los más bajos durante la etapa de reposo. Todos los índices estimados y los parámetros bioquímicos determinados, se correlacionaron significativamente (P < 0,05 con el fotoperiodo, mientras que solo se encontraron correlaciones significativas (P < 0,05 entre el IGS e IGV con respecto a la temperatura del agua.

  6. Desenterrado os mortos: A reconstrução do passado político brasileiro na obra de Edney Silvestre

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    Gabriela Antunes

    2014-07-01

    Full Text Available Este artigo tem como objetivo analisar a trilogia de estreia de Edney Silvestre, composta por Se eu fechar os olhos agora (2009, A felicidade é fácil (2011, e Vidas provisórias (2013. Pretende-se fazer um breve balanço da contribuição do autor para a reabertura de um debate sobre o recente passado histórico-político brasileiro, e, relacionando os fatos abordados no romance com temas contemporâneos, ressaltar a relevância e a atualidade desse debate. Para isso, os textos serão analisados cronologicamente, o que visa mostrar o desenvolvimento do tratamento do sujeito histórico no conjunto de obras do autor. This paper aims to analyse Edney Silvestre’s trilogy composed by If I close my eyes now (2009, Happiness is easy (2011, and Temporary lives (2013, as well as to review the author’s contribution in reopening a debate on Brazilian’s recent historical and political past. By pointing out the relationship between the facts mentioned in thenovels and contemporary Brazil, it aims to stress the importance and up-to-dateness of this discussion. In order to achieve these goals, the novels will be analysed chronologically, which also intends to reveal the development of the way the author deals with Brazilian history throughout his oeuvre.

  7. Ácaros Asociados a Mamíferos y Aves Silvestres de la Reserva Nacional Forestal Bosque de Yotoco -resumen-

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    A Rueda-Hurtado

    2014-12-01

    Full Text Available Colombia posee cerca del 10% de la biota mundial‚ ubicándola segunda entre los doce países megadiversos; con el fin de conocer la diversidad de ácaros asociados a mamíferos y aves silvestres de la Reserva Nacional Forestal Bosque de Yotoco‚ un ecosistema con múltiples amenazas antropicas‚ se realizó este trabajo‚ se colectaron 22 hospederos‚ teniendo en cuenta los protocolos de bioseguridad‚ ética y bienestar animal y de manejo propio de cada especie. Para la captura de los hospederos se utilizaron trampas Sherman y Redes de Niebla‚ para la extracción de los ácaros se utilizó el método de observación directa en estereoscopio‚ montándose en medio Hoyer’s. Para las determinaciones taxonómicas se usaron las claves de Dowling y colaboradores‚ Ohio State University. En el estudio se encontraron 652 ácaros los suborden‚ Astigmata‚ 4 familias‚ representado principalmente por la familia Lardoglyphidae con un 95% (316‚ seguido por Pteronyssidae con el 3% (9‚ Analgidae con 1%(4 y Dermoglyphidae con 1% (3; Mesostigmata‚ segundo suborden más representado‚ se encontraron 237 individuos de 4 familias‚ Laelapidae con 86% (205‚ Macronyssidae con 7% (16‚ Ascidae con 1% (1 y Dermanyssidae con 6% (15. El suborden menos representado fue el Prostigmata con solo una familia Erythraeidae 100% (83.

  8. Endoparasitos (Nemathelminthes y Platyhelminthes de animales de vida silvestre de la Reserva de Biósfera del Manu, Perú

    Directory of Open Access Journals (Sweden)

    Manuel Tantaleán

    2013-06-01

    Full Text Available Durante el año 2001, se recolectaron 8 géneros de helmintos de mamíferos y aves silvestres del Perú, en la Reserva Biosfera del Manu (Departamentos de Cusco y Madre de Dios, desde los 470 a 3780 m. Los parásitos fueron fijados con alcohol etílico en el campo y transportados al laboratorio para estudiarlos; los nemátodos se diafanizaron y los céstodos y tremátodos se colorearon con carmín de acuerdo a técnicas convencionales. La identificación específica de algunos helmintos fue imposible por el estado en que se encontraban, pero se dan a conocer por ser material de gran importancia y ser algunos de ellos algunos registros nuevos para el Perú. Nuestros hallazgos son los siguientes: Rhopalias caballeroi Kifune y Uyema, 1982, Rhopalias sp. (probable n. sp., Taenia pisiformis Bloch, 1780 (cisticerco, Mesocestoides sp. (tetratyridia, Aprocta sp., Evaginuris branicki Mc Clure, 1932, Haematospiculum sp., Protospirura sp., Diplotriaena sp., Thelazia sp. 1, Thelazia sp. 2, Thelazia sp. 3 y Subulura sp.

  9. Híbridos somáticos obtenidos por fusión de protoplastos entre Solanum tuberosum L. subsp. tuberosum y la especie silvestre Solanum circaeifolium Bitter

    Directory of Open Access Journals (Sweden)

    Rosa Espejo

    2013-03-01

    Full Text Available Con la finalidad de obtener híbridos somáticos interespecíficos, se fusionaron protoplastos de la especie tetraploide Solanum tuberosum y de la especie silvestre diploide Solanum circaeifolium utilizando polietilenglicol. Los productos de fusión fueron cultivados en el medio V-KM suplementado con albúmina de suero bovino. Las primeras divisiones celulares ocurrieron a los 3 a 4 días de cultivo. Después de la formación de colonias se observó una rápida proliferación de callos, a partir de los cuales se regeneraron 19 plantas. El análisis molecular usando RAPD, confirmó que los regenerantes presentaban segmentos de ADN de ambos parentales, sugiriendo su posible naturaleza de híbridos somáticos. Las observaciones del número de cromosomas indicaron que todos los híbridos fueron aneuploides. En condiciones de invernadero, los regenerantes derivados de la fusión de protoplastos, mostraron características morfológicas intermedias entre las líneas parentales. Este estudio muestra la producción de híbridos somáticos de papa con el método de fusión presentado.

  10. Índice de desarrollo y supervivencia de larvas del camarón azul Litopenaeus stylirostris (Stimpson, 1871), alimentadas con diferentes concentraciones de Chaetoceros calcitrans (Paulsen)

    OpenAIRE

    Daniel E Godínez; Arnulfo H Díaz; María del C Gallo

    2005-01-01

    Se llevaron a cabo ensayos alimenticios con larvas del camarón azul (Litopenaeus stylirostris) utilizando cuatro diferentes concentraciones de la microalga Chaetoceroscalcitrans (30000, 60000, 90000 y 120000 cél/ml), con una concentración constante por tratamiento de 10000 cél/ml de Tetraselmis suecica y seis nauplios por ml de Artemia franciscana recién eclosionada. El período de prueba se inició a partir del sub estadio zoea 1 y concluyó hasta la aparición de misis 3. En cada ensayo fueron...

  11. Combined effect of exposure to ammonia and hypoxia on the blue shrimp Litopenaeus stylirostris survival and physiological response in relation to molt stage

    OpenAIRE

    Mugnier, Chantal; Zipper, Etienne; Goarant, Cyrille; Lemonnier, Hugues

    2008-01-01

    The effect of ambient ammonia, hypoxia and combination of both on survival and the physiological and immunological response of the blue shrimp Litopenaeus stylirostris in relation to molt stage was studied. Shrimp were submitted to 44.0-71.5 mg 1(-1) total ammonia-N corresponding to 2.0 mg 1(-1) unionized ammonia NH3-N and/or to 1.5 mg O-2 1(-1) (4.3 kPa) for 24 hours. Survival was recorded and the molt stages of both dead and surviving shrimp determined. Only shrimp in intermolt and premolt ...

  12. Evaluación in vivo del efecto cicatrizante de un gel a base de quitosano obtenido de exoesqueleto de camarón blanco litopenaeus vannamei

    OpenAIRE

    Heimy Franceline Martínez Sánchez; Amada Yerén Escobedo Lozano; Evaristo Méndez-Gómez; Alfredo Emmanuel Vázquez; Manuel de Jesús Sol Hernández; Anahí Elizabeth Osuna Lizárraga

    2014-01-01

    Healing effect of a chitosan-based gel obtained from the exoskeleton of white shrimp Litopenaeus vannamei and its evaluation in vivoTítulo corto: Evaluación del quitosano como cicatrizanteResumen:  En México alrededor del 62 % de la población sufre de accidentes causantes de alteraciones en la piel como quemaduras, heridas y diabetes principalmente. Para atender esta problemática, se propone el uso de un gel de quitosano, obtenido del exoesqueleto de camarón ya que presenta actividad antimicr...

  13. Acute toxicity of pyrazosulfuron-ethyl and permethrin to juvenile Litopenaeus vannamei =Toxicidade aguda de pirazossulfurom-etílico e permethrin em juvenis de camarão branco Litopenaeus vannamei

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    Giovanni Lemos de Mello

    2011-01-01

    Full Text Available The objective of this study was to determine the LC50 (96h of two pesticides: SiriusR 250 SC herbicide of the pyrazosulfuron-ethyl group, and TalcordR insecticide of the permethrin group, on juvenile Litopenaeus vannamei. Shrimp total hemocyte count (THC was also determined as an indication of physiological alterations caused by the pesticides. Juvenile shrimp (5.0 } 0.5 g were exposed to the following concentrations: 0, 0.1, 1.0, 10, 100 and 1000 ƒÊg L-1 SiriusR 250 SC; and 0, 0.001, 0.01, 0.1, 1.0 and 10 ƒÊg L-1 TalcordR. The TalcordR LC50 (96h was of 0.00933 ƒÊg L-1 or 9.33 ng L-1. There were no significant changes in the THC between control and test groups. No SiriusR 250 SC concentrations tested killed more than 50%of the shrimp; therefore, the herbicide was considered not toxic to the juveniles. However, the THC showed significant differences between the control and test groups, suggesting sublethal effects to L. vannamei juveniles. According to the results, the insecticide TalcordR is highly lethal for L. vannamei and the herbicide SiriusR 250 SC was not lethal in the concentrations tested but showed sublethal effects as lower THC. The results demonstrate the risks involved in farming L. vannamei shrimp near rice cultures where these pesticides are routinely used.O objetivo deste trabalho foi determinar a CL50 (96h dos agroquimicos SiriusR 250 SC, herbicida a base de pirazossulfurom-etilico, e TalcordR, inseticida a base de permethrin, em juvenis de Litopenaeus vannamei, bem como avaliar possiveis alteracoes fisiologicas por meio da contagem total de hemocitos (CTH dos camaroes. Juvenis de L. vannamei (5,0 } 0,5 g foram expostos as seguintes concentracoes dos agroquimicos: SiriusR 250 SC, 0; 0,1; 1; 10; 100 e 1.000 ƒÊg L-1, e TalcordR, 0; 0,001; 0,01; 0,1; 1 e 10 ƒÊg L-1. A CL50 (96h do inseticida TalcordR foi de 0,00933 ƒÊg L-1 ou 9,33 ng L-1. Nao houve alteracoes significativas da CTH entre as medias dos grupos

  14. Efeito do tipo de sedimento na eficiência alimentar, crescimento e sobrevivência de Litopenaeus vannamei (Boone, 1931 = Effect of sediment type on feed efficiency, growth and survival of Litopenaeus vannamei (Boone, 1931

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    Daniele Bezerra dos Santos

    2011-09-01

    Full Text Available Este estudo avaliou o desempenho zootécnico do camarão marinho Litopenaeus vannamei em diferentes tipos de substratos não consolidados. Juvenis (0,97 ± 0,27 g foram aclimatados na densidade de 52 m-2, em 21 caixas de polietileno com biofiltros individuais, emum sistema fechado de filtração contínua, sendo submetidos aos tratamentos: 1 A = 25% silte + 25% argila + 25% areia muito fina + 25% areia fina; 2 B = 50% areia fina + 50% areia muito fina e 3 Controle = sem substrato, em um delineamento experimental completamente casualizado, com três tratamentos e sete repetições cada. Os animais foram alimentados com 12% da biomassa/dia com ração peletizada (35% de proteína bruta, ofertada em bandejas e parceladaem duas ofertas diárias (8 e 16h. O estudo teve duração de 48 dias, sendo avaliados: consumo aparente de ração, eficiência alimentar, taxa de crescimento específico, sobrevivência e ganho de peso. Foram acompanhados diariamente salinidade, pH, amônia e temperatura da água. A análise estatística dos dados se deu por meio dos testes Anova ou Kuskal-Wallis, em função da sua parametricidade. Não foram observadas diferenças significativas com relação aos fatores avaliadosnas diferentes granulometrias de sedimento testadas, indicando que estas não exerceram influência sobre o desempenho zootécnico de L. vannamei juvenil.This study aimed to evaluate the zootechnical performance of Litopenaeus vannamei shrimp at different types of unconsolidated substrate. Juvenile shrimp (0.97 ± 0.27 g were acclimatized, in a stocking density of 52 m-2, to 21 polyethylene boxes (50 L connected individually to biofilters in a closed water exchange system. The animals were submitted to the treatments: 1 A = 25% silt + 25% clay + 25% very fine sand + 25% fine sand; 2 B = 50% fine sand + 50% very fine sand and 3 Control = no sediment, with seven replications each. Photoperiod was set on 12L: 12D. Shrimps were fed twice a day (at08:00 and

  15. Desempeño reproductivo de dos líneas de reproductores de camarón blanco Litopenaeus vannamei introducidos en Cuba - Reproductive behaviour of broodstocks from lines of white shrimp Litopenaeus vannamei introducing in Cuba

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    Lic. Missael Guerra

    2010-07-01

    Full Text Available ResumenUno de los mayores problemas en los laboratorios de producción depostlarvas es el “inbreeding”, el que puede afectar las características reproductivas de los animales y tener un impacto negativo en la producción. En este trabajo se evaluaron los registros reproductivos de los progenitores de dos introducciones (I1 e I3 de camarón blanco Litopenaeus vannamei provenientes del mismo lugar mantenidos en ciclo de producción cerrado. Para el estudio se tomaron los resultados del número de hembras maduras diarias (Mad/día; Porciento de cópula (% Cop y nauplios por desove (Nau/Des. Todos estos indicadores fueron comparados entre meses y entre introducciones. El número de hembras maduras fue el único que mostró una distribución normal variando de 119 a 178 y de 43 a 70 para cada una de las introducciones respectivamente. Se observó que existen diferencias significativas entre los meses en todos para los indicadores. Se observó un comportamiento similar de loscaracteres reproductivos, el segundo y tercer mes fueron los mejoresdurante el período de producción. No existieron diferencias significativas en el porciento de cópula ni en el número de nauplios por desoves, pero en el número de hembras maduras si hubo diferencias mostrando valores de 160 para I1 y de 62 para I3; esto probablemente se debe a problemas de manejo, el número de animales que se encontraban en la nave o a condiciones ambientales. Estos parámetros son muy importantes para la diferenciación genética entre líneas, además de ser muy útiles en un posible tratamiento de loci cuantitativo (QTL, por sus siglas en ingles que permitan mejoras los caracteres reproductivos y así obtener reproductores más eficientes.SummaryOne of the major concerns in hatcheries is inbreeding, which can affect fitness-related traits, and may have a significant negative impact on production. We present the analysis of reproductive records for broodstocks from two different

  16. Oxygen consumption of Litopenaeus vannamei juveniles in heterotrophic medium with zero water exchange Consumo de oxigênio de juvenis de Litopenaeus vannamei em meio heterotrófico sem renovação de água

    Directory of Open Access Journals (Sweden)

    Luis Vinatea

    2009-05-01

    Full Text Available This work aimed at determining the dissolved oxygen consumption rate of Litopenaeus vannamei juveniles maintained in a microbial biofloc raceway system at high density with no aeration. Three 4 L bottles were filled for each treatment, sealed hermetically, and placed in an enclosed greenhouse raceway system. Four shrimp (13.2±1.42 g were assigned to two sets of the bottles, which underwent the following treatments: light conditions with no shrimp; dark conditions with no shrimp; light conditions with shrimp; and dark conditions with shrimp. Dissolved oxygen content was measured every 10 min for 30 min. A quadratic behavior was observed in dissolved oxygen concentration over time. Significant differences for oxigen consumption were observed only at 10 and 20 min between shrimp maintained in the dark and those under light conditions. At 10 min, a higher value was observed in shrimp maintained under light, and at 20 min, in the dark. Significant differences between 10 and 20 min and between 10 and 30 min were observed when oxygen consumption was analyzed over time in the presence of light. Under dark conditions there were significant differences only between 20 and 30 min. Lethal oxygen concentration (0.65 mg L-1 would be reached in less than one hour either under light or dark conditions with no aeration.O objetivo deste trabalho foi determinar o consumo de oxigênio dissolvido (OD de juvenis de Litopenaeus vannamei mantidos em sistema de cultivo de bioflocs bacterianos em alta densidade e ausência de aeração. Três garrafas de 4 L foram preenchidas para cada tratamento, fechadas hermeticamente e colocadas em sistema de cultivo fechado. Quatro camarões (13,2±1,42 g foram colocados em dois dos conjuntos de garrafas. Os tratamentos aplicados foram: luminosidade, sem camarões; escuro, sem camarões; luminosidade, com camarões; escuro, com camarões. A concentração de oxigênio dissolvido foi determinada a cada 10 min durante 30 min. Foi

  17. Desarrollo del producto brochetas a partir de camaron l. vannamei.

    OpenAIRE

    Rodríguez, G.M.; Flores, E. R.; Espino, R.; Nodarse, Ma. de L.; Turiño, M.

    2010-01-01

    El sector pesquero del Ministerio de la Industria Alimenticia tiene identificada las producciones de camarón entero, colas de camarón, colas peladas de camarón y camarón partido, todos congelados; a partir de camarón de cultivo. con fines exportables ó comercialización en el mercado de frontera y pescaderías especializadas Precisamente el desarrollo de la camaronicultura ha traído consigo una disminución de los precios en el mercado internacional, debido a la saturación de los mismos po...

  18. Growth and Feed Efficiency of Juvenile Shrimp Litopenaeus vannamei Fed Formulated Diets Containing Different Levels of Poultry by-Product Meal

    Institute of Scientific and Technical Information of China (English)

    CHI Shuyan; TAN Beiping; MAI Kangsen; ZHENG Shixuan

    2009-01-01

    This feeding trial was conducted to evaluate the potential of poultry by-product meal (PBM) as a protein source in the culture ofLitopenaeus vannamei. Seven isonitrogenous and isoenergetic diets were formulated to near to commercial diet with about 40% protein and 7.5% lipid. Fish meal was replaced by 0, 30%, 40%, 50%, 60%, 70% and 100% of PBM (diets 1-7). The diet with 100% fish meal was used as a control (diet 1). Post-larvae were reared in an indoor semi-closed re-circulating system. Each dietary treatment was tested in 4 replicate tanks (260 L) of 40 shrimp, arranged in a completely randomized design. The shrimps were hand-fed for three times a day to near-satiation (0700, 1200 and 1800) for 60d. Percentage weight gain, survival, feed conversion ratio (FCR), protein efficiency ratio (PER) and body composition of shrimps were measured. There were no significant differences (P>0.05) in growth performance among shrimps fed diets 1-5 (0-60% fish meal replacement). However, shrimps fed diet 7 (100% fish meal replacement) had significantly lower (P0.05) among different experimental diets. No differences in body composition were found among shrimps fed different diets. These results showed that up to 70% of fish meal protein can be replaced by PBM without adversely affecting the growth, survival, FCR, PER and body composition of Litopenaeus vannamei.

  19. Molecular cloning and characterisation of a pattern recognition molecule, lipopolysaccharide- and beta-1,3-glucan binding protein (LGBP) from the white shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Cheng, Winton; Liu, Chun-Hung; Tsai, Chiung-Hui; Chen, Jiann-Chu

    2005-04-01

    A lipopolysaccharide- and beta-1,3-glucan binding protein (LGBP) cDNA was cloned from the haemocyte and hepatopancreas of white shrimp Litopenaeus vannamei using oligonucleotide primers and RT-PCR. Both 3'- and 5'-regions were isolated by rapid amplification of cDNA end RACE method. Analysis of nucleotide sequence revealed that the cDNA clone has an open reading frame of 1101 bp encoding a protein of 367 amino acids including a 17 amino acid signal peptide. The calculated molecular mass of the mature proteins (350 amino acids) is 39.92 kDa with an estimated pI of 4.37. Two putative integrin binding motifs (cell adhesion site), RGD (Arg-Gly-Asp) and a potential recognition motif for beta- (1-->3) linkage of polysaccharides were observed in the LGBP. Sequence comparison showed that LGBP deduced amino acid of L. vannamei has an overall similarity of 95%, 92% and 61% to that of blue shrimp Litopenaeus stylirostris LGBP, tiger shrimp Penaeus monodon BGBP and crayfish Pacifastacus leniusculus LGBP, respectively. Quantitative real-time RT-PCR analysis showed that LGBP transcript in haemocyte of L. vannamei increased in 3- and 6-h post Vibrio alginolyticus injection. PMID:15561560

  20. Ataque de populações humanas por triatomíneos silvestres no Amazonas: uma nova forma de transmissão da infecção chagásica?

    Directory of Open Access Journals (Sweden)

    José Rodrigues Coura

    1994-12-01

    Full Text Available Comprovou-se o "ataque" de triatomineos silvestres (Rhodnius brethesi a colhedores de piaçava (Leopoldinia piaçaba, em piaçabal na localidade de Acuquaia, no Rio Padauari, afluente do Rio Negro, no Município de Barcelos, Estado do Amazonas, onde em trabalho anterior verificamos uma prevalência sorológica para anticorpos anti-T. cruzi em 12,5% da população na sede do município, com uma forte associação do contato dessa população com triatomineos silvestres, conhecidos na área como "piolho da piaçava", os quais vivem predominantemente nas piaçabeiras. O "ataque " de triatomineos a populações humanas foi possivelmente o mecanismo inicial de abordagem dos triatomineos ao homem, adaptando-se posteriormente ao seu peridomicílio e domicílio, principalmente em áreas desmaiadas ou de cerrado com poucos reservatórios silvestres. No caso do Amazonas, relatado neste trabalho, os triatomineos estavam provavelmente famintos por escassez de outros animais de sangue quente para sua alimentação.An "attack" of wild triatomines (Rhodnius brethesi topiaçaca workers (Leopoldinia piaçaba is confirmed in the locality of Acuquaia, at Padauari river, affluent of Rio Negro in the municipality of Barcelos, State of Amazonas, Brazil. A serological prevalence of 12.5% for T. cruzi antibodies in human population, in the city of Barcelos, has already been described in a previous paper. A strong association between the serological positivity and the population contact with wild triatomines, known in the area as "piaçava's lice", was verified.

  1. Ecología trófica del jabalí europeo (Sus scrofa silvestre en Chile Trophic ecology of the wild boar (Sus scrofa in Chile

    Directory of Open Access Journals (Sweden)

    ÓSCAR SKEWES

    2007-09-01

    Full Text Available Documentamos la dieta del jabalí silvestre en el centro-sur de Chile basados en el examen de 20 estómagos colectados en los faldeos de volcán Mocho-Choshuenco (39°54' S, 72°02' O y en el Parque Nacional Vicente Pérez Rosales (41°03' S, 71°54' O. La dieta está representada por animales, vegetales y hongos. Entre los vegetales y hongos son consumidas tanto las partes epígeas como hipógeas, lo mismo que frutos y semillas. Hongos y rizomas de Gunnera tinctoria constituyen ítemes vegetales cuantitativamente importantes en la alimentación. El jabalí forrajea en general en ambiente boscoso, pero las especies vegetales más frecuentes en la dieta se encuentran en espacios abiertos o fuera del bosque. El ambiente dominado por Chusquea spp. constituye un importante sitio de alimentación. Los roedores de la familia Muridae dominan entre los animales determinados en la dieta del jabalí. Esta situación, sin embargo, parece obedecer a la ocurrencia de una ratada producto de la semillación masiva de Chusquea spp. durante la época de muestreo. Las aves de la familia Rhinocryptidae e invertebrados (en especial larvas del coleóptero Chiasognathus grantii constituyen parte frecuente de los ítemes animales de la dieta del jabalí. En comparación a estudios en otras partes del mundo, el jabalí silvestre en Chile consume hongos en porcentaje de ocurrencia similar (65 % a su dieta en Estados Unidos de América pero bastante más alta que su dieta en Europa (5-32 %. En relación al volumen del componente animal, el jabalí en Chile consume más (16,1 % que en Estados Unidos de América (6 % y que en Europa (7-13 %We document the diet of wild boar in south-central Chile based on analysis of 20 stomachs collected on the outskirts of Mocho-Choshuenco volcano (39°54' S, 72°02' W and of Vicente Pérez Rosales National Park (41°03' S, 71°54' W. The diet incorporates animals, plants and fungi. Among the latter two, both epigeal and hypogeal parts

  2. Frutos tropicais silvestres e polpas de frutas congeladas: atividade antioxidante, polifenóis e antocianinas Wild fruits and pulps of frozen fruits: antioxidant activity, polyphenols and anthocyanins

    Directory of Open Access Journals (Sweden)

    Eugenia Marta Kuskoski

    2006-08-01

    Full Text Available Os sucos de frutas tropicais conquistam cada vez mais o mercado consumidor, sendo o Brasil um dos principais produtores. Existe grande diversidade de produtos derivados de frutos e constante inserção de novos produtos no mercado de consumo, os quais, na maioria das vezes, ainda não foram devidamente pesquisados com respeito às suas propriedades e atividades benéficas à saúde. Neste trabalho, objetiva-se determinar algumas propriedades de frutos tropicais silvestres in natura e polpas de frutos comercializados congelados. Para determinar a atividade antioxidante, utilizou-se o método do radical 2,2-difeniL-1-picrilhidrazilo (DPPH·; para determinar os polifenóis totais, o método de Folin-Ciocalteu e, para as antocianinas, o método da diferença de pH. As polpas de frutas analisadas foram de amora, uva, açaí, goiaba, morango, acerola, abacaxi, manga, graviola, cupuaçu e maracujá; e os frutos silvestres, jambolão e baguaçu. Representada em TEAC (atividade antioxidante equivalente a Trolox, a atividade antioxidante global das polpas oscila entre mínimos e máximos 0,5 e 53,2mimol g-1, enquanto que, representada em VCEAC (atividade antioxidante equivalente a vitamina C, entre mínimos e máximos de 64,8 e 1198,9mg100g-1. Para os extratos de polpa dos frutos in natura, a atividade antioxidante oscila entre 13,3 e 111,2mimol g-1 (TEAC e entre 42,8 e 2533,1mg 100g-1 (VCEAC. Em ordem decrescente de capacidade antioxidante, encontra-se: acerola> manga> morango> uva> açaí> goiaba> amora> graviola> maracujá> cupuaçu> abacaxi. Os frutos de baguaçu apresentam maior atividade antioxidante que os de jambolão.The tropical fruit juices are gaining ever greater space in the consumer market, and Brazil is one of the main producer countries in this market. There is a great diversity of products derived from fruits and new products for consumption are launched constantly, often without the necessary research into their active properties and

  3. Santuario de fauna silvestre cuevas de Paraguaná, Venezuela: lineamientos técnicos para su diseño

    Directory of Open Access Journals (Sweden)

    Pedro Delfín

    2011-01-01

    Full Text Available En este trabajo, se describen los lineamientos técnicos utilizados para el diseño del Santuario de Fauna Silvestre Cuevas de Paraguaná, estado Falcón, Venezuela, cuyo objetivo contempla la protección de un conjunto de cavernas o simas que actúan como refugios y sitios de reproducción de colonias de murciélagos insectívoros y nectarívoros; estos animales desempeñan un papel clave en la ecología y socioeconomía de ambientes xerofíticos en el norte de Venezuela y otras regiones vecinas. La figura incluye cuatro núcleos, cada uno representado por una caverna circundada por una zona de amortiguamiento de forma cuadrada; dos de estas cavernas se ubican en el sector oriental de la península de Paraguaná y el resto en el lado occidental. Se discuten los principales aspectos ecológicos vinculados con la quiropterofauna que habita el Santuario (los cuales fundamentan su decreto, así como los instrumentos legales que sustentan la propuesta y el escenario socioeconómico que caracteriza al área de estudio; adicionalmente, se describen algunos procedimientos seguidos para la incorporación de actores locales en un programa de sensibilización y educación que sirvió como plataforma para la creación de la figura, presentada en un documento técnico, que fue sometido a consideración de las instituciones gubernamentales con competencia en esta materia

  4. Observações sobre calazar em Jacobina, Bahia. VI - Investigações sobre reservatórios silvestres e comensais

    Directory of Open Access Journals (Sweden)

    I. A. Sherlock

    1988-03-01

    Full Text Available Durante os anos de 1982 a 1986, a investigação sobre mamíferos comensais e silvestres, da periferia da cidade de Jacobina, Bahia, mostrou, ao lado do escasso número de exemplares, uma reduzida variedade específica dessa fauna. Capturou-se apenas 11 espécies, entre as quais, predominou o Didelphis albiventris, que abrangeu 44% dos 213 espécimens capturados. Entre os 193 com exames já concluídos, 84 eram exemplares de D. albiventris e 2 estavam infectados pela Leishmania donovani senso lato, 1 por L. mexicana amazonensis, 1 por L. braziliensis, subespécie e 3 por Trypanosoma cruzi Também foram observadas formas suspeitas de serem amastigotas de leishmanics, nos esfregaços de órgãos de 3 exemplares de Dasyprocta aguti, 1 Cercomys cunicularius - e 1 Oryzomys eliurus. 0 restante dos exemplares, inclusive 14 de Lycalopex vetulus, estava negativo para flagelados. Apesar de reforçado por outros indicadores epidemiológicos, como a predominância específica, a freqüência domiciliar, a atratividade para a vetora Lutzomyia longipalpis, e a concomitância com casos humanos nos mesmos locais, o índice de 2,3% de infecção natural do Didelphis albiventris, não autoriza a conclusão definitiva de ser o marsupial o mais importante reservatório natural da leishmaniose visceral em Jacobina.

  5. Diversidad y aspectos biológicos de abejas silvestres de un ambiente urbano y otro natural de la región central de Santa Fe, Argentina

    Directory of Open Access Journals (Sweden)

    Milagros DALMAZZO

    2010-01-01

    Full Text Available El objetivo del presente trabajo fue relevar el elenco de abejas silvestres en la región central de Santa Fe y comparar la diversidad de especies y aspectos biológicos en dos ambientes de una misma región. Las abejas fueron colectadas en el estrato herbáceo de la Reserva Universitaria “Escuela Granja” de la localidad Esperanza, y en la zona urbana de la localidad Susana, ambas ubicadas dentro de la Provincia Fitogeográfica del Espinal. La colecta se realizó en octubre, noviembre y diciembre de 2004 y en enero, febrero y marzo de 2005, registrando las especies vegetales sobre las que se capturaron. Se colectaron 277 ejemplares en total, de los cuales se identificaron 62 especies (27 géneros en 5 familias. Las curvas de rarefacción indican una tasa de acumulación de especies mayor para la Reserva Ecológica, los índices de diversidad de Shannon son 3,25 para la Reserva Ecológica y 2,64 para la Zona Urbana. Para la comparación de las comunidades en ambos sitios, las abejas fueron clasificadas en tres categorías según la especialización en el uso del recurso floral, sustrato de nidificación y comportamiento social. Se observó una asociación entre los grupos especialistas y de nidificación en el suelo con el ambiente natural, y por otro lado, las especies generalistas y de nidificación en cavidades con el ambiente urbano. No se identificaron diferencias, en cuanto al tipo de comportamiento social de las especies, entre ambos sitios.

  6. Epidemiología molecular de la rabia urbana en Colombia, 1994-2004. La rabia en ecosistemas silvestres como amenaza para la población humana

    Directory of Open Access Journals (Sweden)

    Jorge Boshell

    2004-03-01

    Full Text Available

    En Colombia la rabia ocurre en dos ciclos epidemiológicos principales: la rabia urbana con el perro como principal reservorio y transmisor principalmente a humanos, y la rabia silvestre con especies animales de la vida silvestre como los quirópteros y zorros entre otros, como reservorios y transmisores principalmente a especies ganaderas y eventualmente a humanos que se convierten en víctimas accidentales. Durante los últimos años la rabia silvestre en Colombia ha cobrado mayor relevancia como problema de salud pública que la rabia urbana, a juzgar por el número de víctimas humanas. Con el fin de perfeccionar el diagnóstico y la tipificación del virus de la rabia en Colombia, el Laboratorio Nacional de Referencia del Instituto Nacional de Salud estandarizó técnicas para amplificar y secuenciar un fragmento de ADN complementario (cADN a una fracción de 902 nucleótidos seleccionados del ARN del virus, con el fin de adelantar estudios de epidemiología molecular. La fracción mencionada contiene secuencias que codifican para los aminoácidos 447-525 de la glicoproteína y 1-35 de la proteína L. Además contiene la región intergénica no codificante conocida como Pseudogen Psi.

    Las técnicas estandarizadas fueron las siguientes: a extracción de ARN total a partir de cerebro de ratón infectado. b amplificación molecular por transcripción reversa y reacción en cadena de la polimerasa. c secuenciación del fragmento amplificado.

    Mediante estas técnicas de epidemiología molecular viral se determinó que durante 1994-2004, la rabia urbana se concentró en tres regiones geográficas definidas, con epizootias en el antiplano cundiboyacense, departamento de Arauca y la región Caribe. Las dos primeras se controlaron

  7. Sequencing and de novo analysis of the hemocytes transcriptome in Litopenaeus vannamei response to white spot syndrome virus infection.

    Directory of Open Access Journals (Sweden)

    Shuxia Xue

    Full Text Available BACKGROUND: White spot syndrome virus (WSSV is a causative pathogen found in most shrimp farming areas of the world and causes large economic losses to the shrimp aquaculture. The mechanism underlying the molecular pathogenesis of the highly virulent WSSV remains unknown. To better understand the virus-host interactions at the molecular level, the transcriptome profiles in hemocytes of unchallenged and WSSV-challenged shrimp (Litopenaeus vannamei were compared using a short-read deep sequencing method (Illumina. RESULTS: RNA-seq analysis generated more than 25.81 million clean pair end (PE reads, which were assembled into 52,073 unigenes (mean size = 520 bp. Based on sequence similarity searches, 23,568 (45.3% genes were identified, among which 6,562 and 7,822 unigenes were assigned to gene ontology (GO categories and clusters of orthologous groups (COG, respectively. Searches in the Kyoto Encyclopedia of Genes and Genomes Pathway database (KEGG mapped 14,941 (63.4% unigenes to 240 KEGG pathways. Among all the annotated unigenes, 1,179 were associated with immune-related genes. Digital gene expression (DGE analysis revealed that the host transcriptome profile was slightly changed in the early infection (5 hours post injection of the virus, while large transcriptional differences were identified in the late infection (48 hpi of WSSV. The differentially expressed genes mainly involved in pattern recognition genes and some immune response factors. The results indicated that antiviral immune mechanisms were probably involved in the recognition of pathogen-associated molecular patterns. CONCLUSIONS: This study provided a global survey of host gene activities against virus infection in a non-model organism, pacific white shrimp. Results can contribute to the in-depth study of candidate genes in white shrimp, and help to improve the current understanding of host-pathogen interactions.

  8. Cloning, identification and functional analysis of a β-catenin homologue from Pacific white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Zhang, Shuang; Shi, Lili; L, Kai; Li, Haoyang; Wang, Sheng; He, Jianguo; Li, Chaozheng

    2016-07-01

    Wnt signaling is known to control multiple of cellular processes such as cell differentiation, communication, apoptosis and proliferation, and is also reported to play a role during microbial infection. β-catenin is a key regulator of the Wnt signaling cascade. In the present study, we cloned and identified a β-catenin homologue from Litopenaeus vannamei termed Lvβ-catenin. The full-length of Lvβ-catenin transcript was 2797 bp in length within a 2451 bp open reading frame (ORF) that encoded a protein of 816 amino acids. Lvβ-catenin protein was comprised of several characteristic domains such as an N-terminal region of GSK-β consensus phosphorylation site and Coed coil section, a central region of 12 continuous Armadillo/β-Catenin-like repeat (ARM) domains and a C-terminal region. Real-time PCR showed Lvβ-catenin expression was responsive to Vibrio parahaemolyticus and white spot syndrome virus (WSSV) infection. Dual-reporter analysis showed that over-expression of Lvβ-catenin could induce activation of the promoter activities of several antimicrobial peptides (AMPs) such as shrimp PEN4, suggesting that Lvβ-catenin could play a role in regulating the production of AMPs. Knockdown of Lvβ-catenin enhanced the sensitivity of shrimps to V. parahaemolyticus and WSSV challenge, suggesting Lvβ-catenin could play a positive role against bacterial and viral pathogens. In summary, the results presented in this study provided some insights into the function of Wnt/β-catenin of shrimp in regulating AMPs and the host defense against invading pathogens. PMID:27036405

  9. A Dicer-1 gene from white shrimp Litopenaeus vannamei: expression pattern in the processes of immune response and larval development.

    Science.gov (United States)

    Yao, Xuemei; Wang, Lingling; Song, Linsheng; Zhang, Huan; Dong, Chaohua; Zhang, Ying; Qiu, Limei; Shi, Yaohua; Zhao, Jianmin; Bi, Yongkun

    2010-10-01

    Dicer is a member of the RNAase III family which catalyzes the cleavage of double-stranded RNA to small interfering RNAs and micro RNAs, and then directs sequence-specific gene silencing. In this paper, the full-length cDNA of Dicer-1 was cloned from white shrimp Litopenaeus vannamei (designated as LvDcr1). It was of 7636 bp, including a poly A tail, a 5' UTR of 136 bp, a 3' UTR of 78 bp, and an open reading frame (ORF) of 7422 bp encoding a putative protein of 2473 amino acids. The predicted amino acid sequence comprised all recognized functional domains found in other Dicer-1 homologues and showed the highest (97.7%) similarity to the Dicer-1 from tiger shrimp Penaeus mondon. Quantitative real-time PCR was employed to investigate the tissue distribution of LvDcr1 mRNA, and its expression in shrimps under virus challenge and larvae at different developmental stages. The LvDcr1 mRNA could be detected in all examined tissues with the highest expression level in hemocyte, and was up-regulated in hemocytes and gills after virus injection. These results indicated that LvDcr1 was involved in antiviral defense in adult shrimp. During the developmental stages from fertilized egg to postlarva VII, LvDcr1 was constitutively expressed at all examined development stages, but the expression level varied significantly. The highest expression level was observed in fertilized eggs and followed a decrease from fertilized egg to nauplius I stage. Then, the higher levels of expression were detected at nauplius V and postlarva stages. LvDcr1 expression regularly increased at the upper phase of nauplius, zoea and mysis stages than their prophase. The different expression of LvDcr1 in the larval stages could provide clues for understanding the early innate immunity in the process of shrimp larval development.

  10. Impact of the O2 concentrations on bacterial communities and quality of modified atmosphere packaged Pacific white shrimp (Litopenaeus vannamei).

    Science.gov (United States)

    Qian, Yun-Fang; Yang, Sheng-Ping; Xie, Jing; Xiong, Qing; Gao, Zhi-Li

    2013-12-01

    The importance of spoilage-related bacteria in fresh Pacific white shrimp (Litopenaeus vannamei) under different modified atmospheres (MAs) at 4 °C and the effect of O2 were demonstrated in the current study. The changes of bacterial communities in MA-packed shrimp during cold storage were studied by a combined method of plate counts with isolation and identification. Three gas mixtures were applied: 80% CO2 /5% O2 /15% N2, 80% CO2 /10% O2 /10% N2 and 80% CO2 /20% O2, and unsealed packages of shrimp were used as the control. In addition, the TVB-N, pH, whiteness index, and sensory scores were also determined to evaluate the quality changes of shrimp. MA packaging effectively inhibited the increase of total psychrotrophic bacteria counts and H2 S-producing bacteria counts by about 1.7 and 2.1 log cycles, respectively. The growth of Gram-negative spoilage bacteria in shrimp, including Shewanella spp., Aeromonas spp., and Pseudomonas spp., was inhibited by MA packaging, but the growth rate of Gram-positive bacteria such as lactic acid bacteria (LAB) and Brochothrix spp. were less affected by MA as effectively as Gram-negative bacteria. In comparison with the MA-packaged samples, the counts of H2 S producers in shrimp under a CO2 -enriched atmosphere with 20% O2 were slightly lower than the count in samples under an atmosphere with 5% O2 . However, MA with 20% O2 led to higher concentrations of TVB-N, and lower whiteness values and sensory scores. The shelf life of shrimp under 80% CO2 /10% O2 /10% N2 has been prolonged by > 6 d in comparison with the control according to the sensory scores.

  11. Hemolymph cells apoptosis in imported shrimp Litopenaeus vannamei from Hawaii to Iran, exposed to white spot virus

    Institute of Scientific and Technical Information of China (English)

    Zeliha Selamoglu Talas; Mehmet Fuat Gulhan

    2014-01-01

    Objective: To show hemolymph apoptosis in imported shrimp Litopenaeus vannamei from Hawaii to Iran. Methods: One hundred and eighty shrimps [(7.98±0.54) g] which were collected from a research shrimp farm located in Heleh site in north of Bushehr Province were distributed equally to 6 glass aquariums (50 cmí50 cmí60 cm) as group A in triplicate (imported batch in 2011, without crossing with other generations) with well clean aerated sea water (100 L per aquarium), salinity of 40 ‰ and temperature of 29 °C. Shrimps of group B (produced by crossing the adults of imported batches in 2009 up to 2011) were distributed also among 6 aquariums with the same conditions. Both shrimp groups were injected with concentration of LD50=1í105.4 white spot virus.Results:exposure and reached 100% after 36 h but no mortality was occurred up to 15 d in shrimps of group B. The slide evaluation of hemolymph of group B showed an increasing trend of apoptosis occurrence in all three types of hemolymph cells, hyalinocytes, semi-granulocytes and granulocytes from 24 h to 72 h in contrary to group A that not any apoptosis was observed during the course of the study (15 d). The results showed that in group A, the mortality began approximately 24 h after Conclusions: It is concluded that crossing among the specific pathogen free generations could induce the increasing immunity level through apoptosis to protect them against white spot disease.

  12. Injected phage-displayed-VP28 vaccine reduces shrimp Litopenaeus vannamei mortality by white spot syndrome virus infection.

    Science.gov (United States)

    Solís-Lucero, G; Manoutcharian, K; Hernández-López, J; Ascencio, F

    2016-08-01

    White spot syndrome virus (WSSV) is the most important viral pathogen for the global shrimp industry causing mass mortalities with huge economic losses. Recombinant phages are capable of expressing foreign peptides on viral coat surface and act as antigenic peptide carriers bearing a phage-displayed vaccine. In this study, the full-length VP28 protein of WSSV, widely known as potential vaccine against infection in shrimp, was successfully cloned and expressed on M13 filamentous phage. The functionality and efficacy of this vaccine immunogen was demonstrated through immunoassay and in vivo challenge studies. In ELISA assay phage-displayed VP28 was bind to Litopenaeus vannamei immobilized hemocyte in contrast to wild-type M13 phage. Shrimps were injected with 2 × 10(10) cfu animal(-1) single dose of VP28-M13 and M13 once and 48 h later intramuscularly challenged with WSSV to test the efficacy of the vaccine against the infection. All dead challenged shrimps were PCR WSSV-positive. The accumulative mortality of the vaccinated and challenged shrimp groups was significantly lower (36.67%) than the unvaccinated group (66.67%). Individual phenoloxidase and superoxide dismutase activity was assayed on 8 and 48 h post-vaccination. No significant difference was found in those immunological parameters among groups at any sampled time evaluated. For the first time, phage display technology was used to express a recombinant vaccine for shrimp. The highest percentage of relative survival in vaccinated shrimp (RPS = 44.99%) suggest that the recombinant phage can be used successfully to display and deliver VP28 for farmed marine crustaceans.

  13. Management of the fox (Vulpes vulpes in Lombardy Region (Northern Italy in relation to rabies / Interventi sulle popolazioni di Volpe (Vulpes vulpes in Lombardia in relazione al controllo della rabbia silvestre

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    Guido Gerletti

    1991-07-01

    Full Text Available Abstract A description of the sanitary measures adopted in Lombardy Region against the spreading of rabies in populations of foxes is provided. Detailed accounts are reported for the Province of Sondrio. Riassunto Viene fornito un quadro sintetico relativo all'andamento dell'epidemia di rabbia silvestre nel territorio della Regione Lombardia e ai provvedimenti di controllo sanitario adottati nei confronti delle popolazioni di Volpe. Più in dettaglio è esaminata la situazione in provincia di Sondrio.

  14. Ocorrência de anticorpos anti-Toxoplasma gondii em aves silvestres de três Unidades de Conservação Federais da Paraíba e da Bahia

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    Leontina H.M. Andrade

    2016-02-01

    Full Text Available Resumo: A vigilância e monitoramento de doenças em animais silvestres são imprescindíveis no contexto ambiental e de saúde pública, pois estes animais agem como sentinelas, refletindo alterações ambientais precocemente, o que proporciona maior eficácia no monitoramento ambiental e permite o acesso rápido a informações sobre as condições da área. Neste contexto, as aves são importantes no ciclo biológico do Toxoplasma gondii e na epidemiologia da toxoplasmose, principalmente porque seus tecidos representam importantes fontes de proteína na alimentação de felídeos e humanos. Objetivou-se detectar anticorpos anti-T. gondii, por meio do teste de aglutinação modificada em aves silvestres de três Unidades de Conservação (UC Federais dos Estados da Paraíba e Bahia. No período de dezembro de 2011 a outubro de 2013 foram capturadas com redes de neblina 222 aves silvestres pertencentes a 67 espécies, 27 famílias e 12 ordens. Após a captura, foi colhido sangue de cada animal e separado o soro, que foi submetido ao Teste de Aglutinação Modificada (MAT≥1:25 utilizando taquizoítos inativados na formalina e 2-mercaptoetanol. Dentre as 222 amostras analisadas, três (1,3% foram sororreagentes: 1 de 16 (6,2% pipira-preta Tachyphonus rufus (título 50, 1 de 5 (20% juriti-gemedeira Leptotila rufaxilla (título 50 e 1 de 1 (100% caneleiro-enxofre Casiornis fuscus (título 25. Este é o primeiro relato da ocorrência de anticorpos anti-T. gondii nas referidas espécies de aves silvestres de vida livre nas duas UC Federais estudadas.

  15. Acesso ao alimento artificial e enchimento do trato digestivo de juvenis do camarão marinho Litopenaeus vannamei (Boone (Crustacea, Decapoda, Penaeidae durante as fases clara e escura do período de 24 horas Artificial food access and digestive tract filling of juvenil marine shrimp Litopenaeus vannamei (Boone (Crustacea, Decapoda, Penaeidae during light and dark phases in 24-hour period

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    Cibele S. Pontes

    2005-12-01

    Full Text Available A escassez de dados acerca do comportamento alimentar do camarão marinho Litopenaeus vannamei (Boone, 1931 com relação ao alimento artificial ofertado em bandejas poderá induzir a uma alimentação inadequada, resultando no desperdício da ração, no aporte desnecessário de nutrientes, e conseqüente aumento dos custos com a alimentação e da poluição ambiental potencial desta atividade. Objetivando fornecer subsídios para a melhoria do manejo alimentar praticado nas fazendas, foram realizados estudos comportamentais utilizando 64 juvenis de Litopenaeus vannamei (7,57 ± 1,01g, mantidos em densidade populacional de 33 m², submetidos a fotoperíodo artificial, em ciclo invertido, para observação durante as fases clara e escura. A ração foi ofertada em intervalos pré-estabelecidos, registrando-se posteriormente através de método focal contínuo: a latência para chegada à bandeja, b latência para consumo do alimento e c Índice de Enchimento do Trato Digestivo. Os animais foram mais rápidos para chegar à bandeja e também para iniciar o consumo do alimento nos horários da fase clara. O enchimento do trato digestivo mostrou-se superior na meia hora subsequente à oferta do alimento, especialmente nos horários da fase clara.The few data on the marine shrimp Litopenaeus vannamei (Boone, 1931 behavior related to feed offer in trays can result inadequate feeding by the animal, feed wastage and unnecessary nutrient input, increasing feed costs and potential environmental pollution of that culture. In order to provide tools for improved feed management methods in shrimp farms, a behavioral study was conducted, using 64 Litopenaeus vannamei juveniles (7,57 ± 1,01g, one animal per 33 m². They were submitted to artificial photoperiods, half of them in reversed cycle, in order to register behavior during light and dark phases. The following variables were registered (continuous sampling after feed exposition: a latency to access the

  16. Biorremediación de los efluentes de cultivo del camarón Litopenaeus vannamei (Boone, 1931 utilizando tapetes microbianos en un sistema de recirculación Bioremediacion of effluents ones of the culture of Litopenaeus vannamei (Boone, 1931 using microbial mats in a recirculating system

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    Carlos Lezama-Cervantes

    2010-01-01

    Full Text Available Se desarrolló un sistema de recirculación (SR integrando el cultivo del camarón con tapetes microbianos buscando mitigar los impactos ambientales de los subproductos del cultivo del camarón y avanzar hacia la biorremediación del agua de cultivo. Los consorcios microbianos obtenidos de ambientes naturales (TaM estuvieron constituidos por bacterias (55,6%, cianófitas (18,4%, diatomeas (9%, nemátodos (5,6% y clorófitas (1,4%, entre los grupos taxonómicos principales. La remoción de nutrientes y sólidos se evaluó en un SR (2 ciclos/hora conteniendo (n = 3 60 y 120 ind m-2 de Litopenaeus vannamei. Los resultados demostraron que los TaM redujeron los niveles de nitrógeno amoniacal por encima del 71% diariamente; la demanda bioquímica de oxígeno (DBO5 se redujo más de 68% y los sólidos suspendidos (SST hasta en 62% al compararse con los sistemas control (p 0,05. El agua bio-remediada tiene un efecto positivo en el cultivo de Litopenaeus vannamei, y promueve el crecimiento y sobrevivencia en presencia de TaM en el SR.We developed a recirculating system integrating shrimp culture with microbial mats aiming to mitigate the environmental impacts of by-products from shrimp culture and to advance toward the bioremediation of the shrimp culture water. The microbial consortia collected from natural environments (TaM was constituted by bacteria (55.6%, Cyanophyte (18.4%, diatoms (9%, nematods (5.6% and Chlorophyte algae (1.4% among the major taxonomic groups. The removal of nutrients and solids by these mats was assessed in a recirculating system (2 cycles/hour (n = 3 containing 60 and 120 ind m-2 of Litopenaeus vannamei. Results showed that the microbial mat reduces daily up to 71% of ammonia nitrogen, decreases around 68% of the biochemical oxygen demand (DBO5 and up to 62% of suspended solids (SST when comparing to the control systems (p 0.05. The Bioremediated culture water had a positive effect on the culture of Litopenaeus vannamei, and

  17. Primer registro de la utilización de harinas de Salicornia bigelovii y Scomber japonicus en dietas prácticas para el cultivo súper-intensivo de camarón Litopenaeus stylirostris First record on the use of Salicornia bigelovii and Scomber japonicus fishmeals as feed for Litopenaeus stylirostris under super-intensive farming

    OpenAIRE

    Manuel de J . Acosta-Ruiz; J. Paniagua-Michel; J Olmos-Soto; E Paredes-Escalona

    2011-01-01

    Se evaluó el efecto de las harinas de Salicornia bigelovii (SA) y Scomber japonicus, semiprocesada (HPS) como ingredientes en la formulación de dietas para camarón azul Litopenaeus stylirostris, en cultivo súper-intensivo. Se formularon tres diferentes dietas isoproteicas (40%) e isocalóricas (6 kcal g-1): (DSA), (DHPS), basal (DBA) y una dieta control (DCO). El peso obtenido con DSA y DHPS (0,9 ± 0,014 y 0,8 ± 0,015 g) fue similar a la dieta comercial DCO (0,9 ± 0,07 g), no existieron difere...

  18. Aclimatação do camarão marinho Litopenaeus vannamei (Boone, 1931 à água doce com diferentes estratégias de alimentação e calagem = Acclimatization of the marine shrimp Litopenaeus vannamei (Boone, 1931 to the freshwater with different feeding and liming strategies

    Directory of Open Access Journals (Sweden)

    Paulo de Paula Mendes

    2006-01-01

    Full Text Available Pós-Larvas do camarão Litopenaeus vannamei, na fase de PL9-10 e PL25-26 foram aclimatadas à água doce (0,0 ‰, objetivando promover cultivos em regiões interioranas. Para isso, foram realizados dois experimentos conduzidos em aquários com capacidade útil de 44 litros e densidade de estocagem de 11,36 pós-larvas/L. No experimento 1, foram testadas diferentes concentrações de cal hidratada (0,018 g a 0,031 g e 2 dietas, formuladas à base de ração comercial para camarão e biomassa de artêmia. No experimento 2, foram testadas 5dietas com diferentes concentrações de biomassa de artêmia (0% a 100%, inseridas em rações de peixes e camarões. No final do processo de aclimatação, a sobrevivência das póslarvas variou de 40,44% a 98,04%. Concluiu-se que pós-larvas do Litopenaeus vannamei podem ser aclimatadas à água doce e que o aumento da concentração de cal e de artêmia na ração melhoraram significativamente (pLitopenaeus vannamei postlarvae with 9-10 (PL9-10, 25-26 days old (PL25-26 were acclimated to freshwater (0 ppt for the purpose of attending the inland cultures. Two experiments were conducted in 44 liter aquariums with stocking density of 11.36 postlarvae/L. In experiment 01, different concentrations of hydrated lime (0.018 g a 0.031 g and two diets, formulated with commercial shrimp ration and artemia biomass, were tested. In experiment 02, five diets, withdifferent concentrations of artemia biomass (0 to 100% inserted in fish and shrimp rations, were tested. At the end of the acclimatization process, the postlarvae survival varied from40.44 to 98.04%. Results showed that Litopenaeus vannamei postlarvae can be acclimated to freshwater. Also, the increase of lime concentration and the artemia biomass in the ration improved (p<0.05 the zootechnic parameters of the species in a significant way.

  19. Optimización del procedimiento del cálculo del alimento en estanques de engorde para la eficiencia del cultivo del camarón blanco Litopenaeus vannamei en Cuba (Optimization of the procedure of calculating the fattening food in

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    Jiménez Cabrera, Redney

    2011-04-01

    Full Text Available ResumenLa alimentación es una práctica de manejo importante si se considera su costoelevado y su efecto nocivo en el ecosistema del estanque. El método másutilizado para alimentar camarones en cultivos es por voleo y la dosis dealimento proporcionada por este método, se determina por tablas dealimentación.SummaryFood is an important management practice considering its high cost and itsharmful impact on the ecosystem of the pond. The method used to feed shrimpcrop is broadcast and the dose of food provided by this method is determinedby feeding tables.

  20. Intoxicaciones intencionadas y accidentales de fauna silvestre y doméstica en España: diferencias entre Comunidades Autónomas

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    IS Sánchez-Barbudo

    2012-01-01

    Full Text Available En este estudio se han analizado 1.157 casos sospechosos de intoxicación de fauna silvestre y doméstica en el medio natural (1.800 animales y 340 cebos procedentes de diversas Comunidades Autónomas (CCAA españolas durante el periodo 2004-2010. Se ha detectado un 41,2% de casos positivos (40,8% de animales y 52,6% de cebos. En los carnívoros domésticos la detección del tóxico llegó al 71,4%, lo que indica su utilidad como centinelas del uso de veneno en el medio natural. El 78,3% de los animales que fueron positivos a los análisis toxicológicos han sido considerados como intoxicaciones intencionadas. Las aves rapaces diurnas fueron el grupo más afectado por las intoxicaciones (43,6% del total de animales positivos, seguido de los mamíferos carnívoros (27,1%. Los tóxicos más frecuentemente detectados fueron insecticidas anticolinesterásicos (cebos/animales: 80,4%/65,8%, seguidos de rodenticidas anticoagulantes (5%/19,6%, estricnina (2,2%/6,5% y arsénico (4,5%/2,3%. De las diferencias observadas entre CCAA destaca la preponderancia en el uso de estricnina en Asturias, rodenticidas anticoagulantes en Castilla y León, insecticidas organofosforados en Aragón, insecticidas carbamatos en Castilla-La Mancha y Madrid, y la aparición de otros venenos, como á-cloralosa o barbitúricos, en Cataluña. En resumen, el 82,3% de las intoxicaciones intencionadas fueron debidas a anticolinesterásicos y el 85,5% de las accidentales a rodenticidas anticoagulantes. En futuras regulaciones de plaguicidas y biocidas se debería tener en cuenta el riesgo del uso ilegal en la preparación de cebos envenenados que comporta la comercialización de formulados con alta riqueza de ingredientes activos con baja DL .

  1. Dispersión endozoócora de leguminosas silvestres: desde la recuperación hasta el establecimiento en campo

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    María Eugenia Ramos-Font

    2015-01-01

    Full Text Available Dispersión endozoócora de leguminosas silvestres: desde la recuperación hasta el establecimiento en campoLa dispersión de semillas mediante el tracto digestivo de los animales constituye un mecanismo esencial para la colonización de nuevos nichosecológicos, el mantenimiento de poblaciones vegetales, y para evitar la fragmentación de hábitats. Este trabajo evalúa la capacidad real de dispersiónendozóoora por ganado ovino de Adenocarpus decorticans (AD, A. telonensis (AT, Cytisus fontanesii (CF, C. striatus (CS, Genista florida (GF yG. versicolor (GV mediante el análisis de: recuperación de semillas en heces, germinación en cámara, emergencia en invernadero y establecimientoy supervivencia en campo durante dos años. Nuestros resultados sugieren que el ganado ovino es un agente de dispersión efectivo para la mayoríade las especies, excepto para CF. La recuperación de semillas osciló entre el 32% (AT y GV y el 3.4% (CS. Se observó que el paso por el tractodigestivo estimulaba o no afectaba a la germinación de las semillas en laboratorio. En invernadero, la mayoría de las especies incrementaban oigualaban su emergencia en las heces respecto a las semillas control. Sin embargo, el estiércol dificultó la germinación de la mitad de las especiesen condiciones naturales. A pesar de ello, la probabilidad de reclutamiento de las semillas dispersadas fue similar o superior a la de semillas intactas,excepto para CF. En conclusión, la oveja puede ser un dispersador eficaz de la mayoría de las especies estudiadas y esta capacidad podría utilizarseen planes de gestión y/o restauración forestal.

  2. Comportamento reológico de méis de florada de silvestre Rheological behavior of honey from Serjania glabrata flowers

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    Alexandre J. de M. Queiroz

    2007-04-01

    Full Text Available Estudou-se o comportamento reológico de méis de Apis mellifera produzidos no estado do Piauí, no semi-árido brasileiro, elaborados com florada predominante de silvestre (Serjania glabrata. As amostras foram coletadas ao longo do ano 2000, a partir de coletas realizadas por apicultores, associações e cooperativas de apicultura da região abrangida pelos municípios de Picos, Itainópolis, Vera Mendes e Isaías Coelho. Após as coletas as amostras passaram pelos processos de centrifugação, filtragem e decantação. As medidas reológicas foram feitas nas temperaturas de 20 a 40 ºC utilizando-se um viscosímetro Brookfield, modelo RVT. As leituras de velocidade de rotação e torque foram transformadas em valores de taxa de deformação e tensão de cisalhamento. Os dados de taxa de deformação e tensão de cisalhamento foram ajustados pelas equações da Lei-da-Potência e de Herschel-Bulkley. As amostras apresentaram comportamento pseudoplástico. As equações se ajustaram adequadamente aos resultados experimentais de tensão de cisalhamento em função da taxa de deformação. O aumento de temperatura reduziu a viscosidade aparente em níveis próximos de 80%. Os valores de viscosidade aparente foram bem ajustados por uma equação do tipo Arrhenius.The rheological behavior of Apis mellifera honey produced in the State of Piauí, in the Brazilian semi-arid, obtained with predominance of Serjania glabrata flowers was studied. The samples were collected in the year 2000 by beekeepers and associations and cooperatives of beekeepers in the area covered by the municipal districts of Picos, Itainópolis, Vera Mendes and Isaías Coelho. The samples were centrifuged, filtered, decanted and the rheological measures at temperatures of 20 to 40 ºC using a Brookfield Viscometer RVT model were made. The readings of rotation speed and torque were transformed in shear rate and shear stress values. The data of shear rate and shear stress were fitted

  3. RESPUESTA DE PLANTAS DE PAPAYA SILVESTRE (Carica cubensis Solms AL ESTRÉS HÍDRICO Y SU RECUPERACIÓN: ASPECTOS FISIOLÓGICOS Y DEL CRECIMIENTO

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    Yusnier Díaz Hernández

    2014-01-01

    Full Text Available Carica cubensis Solms es una especie silvestre endémica de Cuba. Son poco conocidos los aspectos fisiológicos de esta especie en condiciones de humedad limitada del suelo, por lo que el presente trabajo tuvo como objetivo estudiar la respuesta de la misma en la fase vegetativa ante la deficiencia hídrica en el suelo y su recuperación. El estudio se condujo en condiciones semi-controladas, donde las plantas fueron sometidas a 21 y 15 días de supresión y recuperación del riego respectivamente. Se monitoreó la humedad en el suelo, y se evaluaron la altura de las plantas, numero de hojas, superficie foliar, las masas secas de la raíz, tallo y hojas y el contenido foliar de clorofilas. Además, se calculó la Tasa Absoluta de Crecimiento (TAC y Área Foliar Específica (AFE. El contenido de humedad en el suelo disminuyó hasta alcanzar valores de un 12 %, se afectó la altura, la superficie foliar, la biomasa de los órganos y el contenido de clorofilas. La relación biomasa de la raíz/biomasa de la parte aérea tendió al aumento en las plantas estresadas, lo que constituye un posible mecanismo de tolerancia a la sequía. El desarrollo de las plantas, por el comportamiento en el tiempo de la TAC, y el AFE, no sufrió modificaciones. Al establecerse el periodo de rehidratación, todas las variables mostraron una tendencia a la recuperación y, al final del periodo, la superficie foliar, el contenido de contenido de clorofilas totales, así como la TAC de la biomasa de raíz, alcanzaron valores similares al control. Se comprueba que en períodos cortos de estrés, seguido de una recuperación, no se afecta de manera general el crecimiento vegetativo de plantas de Carica cubensis.

  4. Dynamic behavior of sylvatic yellow fever in Brazil (1954-2008 Comportamento dinâmico da febre amarela silvestre no Brasil (1954-2008

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    Fernando Portela Câmara

    2011-06-01

    Full Text Available INTRODUCTION: Sylvatic yellow fever (SYF is enzootic in Brazil, causing periodic outbreaks in humans living near forest borders or in rural areas. In this study, the cycling patterns of this arbovirosis were analyzed. METHODS: Spectral Fourier analysis was used to capture the periodicity patterns of SYF in time series. RESULTS: SYF outbreaks have not increased in frequency, only in the number of cases. There are two dominant cycles in SYF outbreaks, a seven year cycle for the central-western region and a 14 year cycle for the northern region. Most of the variance was concentrated in the central-western region and dominated the entire endemic region. CONCLUSIONS: The seven year cycle is predominant in the endemic region of the disease due the greater contribution of variance in the central-western region; however, it was possible identify a 14 cycle that governs SYF outbreaks in the northern region. No periodicities were identified for the remaining geographical regions.INTRODUÇÃO: A febre amarela silvestre (FAS é enzoótica no Brasil, causando surtos periódicos em humanos que vivem próximos às áreas florestais ou em áreas rurais. Neste estudo, foram analisados os padrões de periodicidade desta arbovirose. MÉTODOS: Utilizamos a análise espectral de Fourier para capturar os padrões de periodicidades da FAS em séries temporais. RESULTADOS: Os surtos de FAS aparentemente não aumentaram em frequência, mas em número de casos. Há dois ciclos dominantes na FAS, um de sete anos predominando na região centro-oeste, e um de 14 anos predominando na região norte. A maior parte da variância concentrou-se na região centro-oeste e dominava toda região endêmica. CONCLUSÕES: O ciclo de sete anos é predominante para a região endêmica da doença devido a maior contribuição da variância do centro-oeste. No entanto, foi possível identificar um ciclo de 14 que rege a FAS na região norte. Não foram detectadas periodicidades nas demais

  5. Fenología de la floración en tomate cultivado y especies silvestres relacionadas Flowering phenology in cultivated tomato and related wild species

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    Edwin Fernando Restrepo Salazar

    2008-06-01

    Full Text Available En un diseño experimental de bloques completos al azar se comparó la fenología de la floración de doce accesiones silvestres de Solanum sección Lycopersicum con la del tomate cultivado tipo “chonto”. Los tratamientos se repitieron tres veces. Se evaluó la aparición de las 12 primeras inflorescencias y el tipo de inflorescencia. Las fenologías de las accesiones de la variedad glabratum de S. habrochaites (PI 134417, PI134418 y PI126449, las accesiones LA1624, LA2092 de la variedad typicum de S. habrochaites y la accesión LA 444-1 de S. peruvianum coincidieron con la del tomate “chonto”. No se presentaron diferencias significativas en la variable días a inicio de floración entre las accesiones de la variedad glabratum y el tomate cultivado. Las accesiones de la variedad glabratum de S. habrochaites y el tomate cultivado presentaron inflorescencias simples; mientras que las accesiones de la variedad typicum de S. habrochaites y de la especie S. peruvianum presentaron inflorescencias bifurcadas.The flowering phenology of twelve wild accesions of Solanum section Lycopersicum were compared with those of the cultivated tomato type of “chonto” by using the randomized complete block design. The treatments were repeated three times. The appearance initiate of the first twelve inflorescences and kind of inflorescence were evaluated. The phenologies of the accesions of the glabratum variety from S. habrochaites (PI 134417, PI134418 y PI126449, LA1624 y LA2092 of the typicum variety from S. habrochaites and LA 444-1 from S. peruvianum coincided with those of the “chonto” tomato. There were no significant differences between the glabratum variety accessions and cultivated tomato in the starting days of flowering variable. The accesions of the glabratum variety from S. habrochaites and the cultivated tomato presented simple inflorescences; while the accesions of the typicum variety from S. habrochaites and S. peruvianum specie showed

  6. Effect of probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress of Litopenaeus stylirostris under Vibrio nigripulchritudo challenge.

    Science.gov (United States)

    Castex, Mathieu; Lemaire, Pierrette; Wabete, Nelly; Chim, Liet

    2010-04-01

    Antioxidant defences and induced oxidative stress tissue damage of the blue shrimp Litopenaeus stylirostris, under challenge with Vibrio nigripulchritudo, were investigated for a 72-h period. For this purpose, L. stylirostris were first infected by immersion with pathogenic V. nigripulchritudo strain SFn1 and then antioxidant defences: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (Gpx), Total antioxidant status (TAS), glutathiones and induced tissue damage (MDA and carbonyl proteins) were determined in the digestive gland at 0, 12, 24, 48 and 72 h post-infection (h.p.i.). In the meantime, TAS was also measured in the blood. Infection level of the shrimps during the challenge was followed by determining V. nigripulchritudo prevalence and load in the haemolymph of the shrimps. Changes in all these parameters during the 72-h.p.i. period were recorded for control shrimps and shrimps previously fed for one month with probiotic Pediococcus acidilactici MA18/5M at 10(7) CFU g(-1) of feed. Our results showed that immersion with V. nigripulchritudo led to maximal infection level in the haemolymph at 24 h.p.i. preceding the mortality peak recorded at 48 h.p.i. Significant decreases in the antioxidant defences were detected from 24 h.p.i. and beyond that time infection leaded to increases in oxidative stress level and tissue damage. Compared to control group, shrimps fed the probiotic diet showed lower infection (20% instead of 45% at 24 h.p.i. in the control group) and mortality (25% instead of 41.7% in the control group) levels. Moreover, infected shrimp fed the probiotic compared to uninfected control shrimps exhibited very similar antioxidant status and oxidative stress level. Compared to the infected control group, shrimps fed the probiotic sustained higher antioxidant defences and lower oxidative stress level. This study shows that bacterial infection leads to oxidative stress in L. stylirostris and highlighted a beneficial effect of P

  7. Effect of Dissolved Oxygen on Swimming Ability and Physiological Response to Swimming Fatigue of Whiteleg Shrimp (Litopenaeus vannamei)

    Institute of Scientific and Technical Information of China (English)

    DUAN Yan; ZHANG Xiumei; LIU Xuxu; Dhanrajsingh N. Thakur

    2014-01-01

    The swimming endurance of whiteleg shrimp (Litopenaeus vannamei, 87.66 mm ± 0.25 mm, 7.73 g ± 0.06 g) was exam-ined at various concentrations of dissolved oxygen (DO, 1.9, 3.8, 6.8 and 13.6 mg L-1) in a swimming channel against one of the five flow velocities (v1, v2, v3, v4 and v5). Metabolite contents in the plasma, hepatopancreas and pleopods muscle of the shrimp were quantified before and after swimming fatigue. The results revealed that the swimming speed and DO concentration were significant factors that affected the swimming endurance of L. vannamei. The relationship between swimming endurance and swimming speed at various DO concentrations can be described by the power model (ν·tb=a). The relationship between DO concentration (mg L-1) and the swimming ability index (SAI), defined as SAI= 90000∫ vdt (cm) , can be described as SAI=27.947 DO0.137 (R2=0.9312). The level of DO concentration directly affected the physiology of shrimp, and exposure to low concentrations of DO led to the increases in lactate and energetic substrate content in the shrimp. In responding to the low DO concentration at 1.9 mg L-1 and the swimming stress, L. vannamei exhibited a mix of aerobic and anaerobic metabolism to satisfy the energetic demand, mainly characterized by the utilization of total protein and glycogen and the production of lactate and glucose. Fatigue from swimming led to severe loss of plasma triglyceride at v1, v2, and v3 with 1.9 mg L-1 DO, and at v1 with 3.8, 6.8 and 13.6 mg L-1 DO, whereas the plasma glucose con-tent increased significantly at v3, v4 and v5 with 3.8 and 6.8 mg L-1 DO, and at v5 with 13.6 mg L-1 DO. The plasma total protein and hepatopancreas glycogen were highly depleted in shrimp by swimming fatigue at various DO concentrations, whereas the plasma lactate accumulated at high levels after swimming fatigue at different velocities. These results were of particular value to under-standing the locomotory ability of whiteleg shrimp and its

  8. Real time PCR quantification of WSSV infection in specific pathogen free (SPF) Litopenaeus vannamei (Boone, 1931) exposed to antiviral nucleotide

    Institute of Scientific and Technical Information of China (English)

    MA Badhul Haq; K Kalai Priya; R Rajaram; R Vignesh; M Srinivasan

    2012-01-01

    Objective: To investigate the level of WSSV transmission from the infected tiger prawn Penaeus monodon (P. monodon) to specific pathogen free Litopenaeus vannamei (L. vannamei) in laboratory captivity condition in relation to PCR detection, histopathological observation and viral genome sequence. Methods: Viral DNA was isolated from purified virions by treatment with proteinase K (0.2 mg/mL) and Sarkosyl (1%). The purity and concentration of the DNA were determined by agarose gel electrophoresis. Moribund and dead shrimp were removed and processed for indirect immunofluorescence (IIF) analysis. Histological observation of infected L. vannamei shrimps were revealed by the degenerated cells which were characterized by intranuclear inclusions in the tissues of WSSV infected mid-gut gland, lymphoid organ, gill lamellae and gut epithelium. Total DNA was extracted, from shrimp hemolymph and tissues, with a High Puree PCR template preparation kit. WSSV-DNA was detected using a commercial 2-step PCR detection kit. Results:The present study compares the real-time PCR results with SYBR Green I concentration ranging from 0.2 to 0.7×. The positive standard was used in the range of 102, 104 106, 108 and 1010 copies/ng of DNA in general. The PCR analysis showed the appearance of a prominent band from the PCR amplified product of WSSV-DNA at internal control band of 848 bp. Moderate and severe levels were observed as 650 bp and 910 bp (200 & 2 000 copies) in various transmission routes. The WSSV content in moribund shrimp of all the experimental species (L. vannamei) approximately ranged in nucleotide application by quantification method from 0.000 001 WSSV copies/μg of total DNA. In whole moribund infection animal, approximately 0.02 WSSV copies/μg of DNA was detected in nucleotide applied animal. Conclusion: These results indicate that wild brood stock and native culture shrimp P. monodon may be infected with WSSV and can get transferred into the SPF L. vannamei farming

  9. Optimal content and ratio of lysine to arginine in the diet of Pacific white shrimp, Litopenaeus vannamei

    Institute of Scientific and Technical Information of China (English)

    FENG Zhengfu; DONG Chaohua; WANG Linlin; HU Yanjiang; ZHU Wei

    2013-01-01

    The optimal quantity of dietary lysine (Lys) and arginine (Arg),and the optimal ratio of dietary Lys to Arg for Pacific white shrimp Litopenaeus vannamei were investigated.Coated Lys and Arg were added to a basal diet (37.99% crude protein and 7.28% crude lipid) to provide graded levels of Lys and Arg.The experimental diets contained three Lys levels (2.51%,2.11%,and 1.70% of total diet),and three Arg levels (1.41%,1.80%,and 2.21% of total diet) and all combinations of these levels were tested.Pacific white shrimp,with a mean weight of 3.62±0.1 g,were randomly distributed in 36 fiberglass tanks with 30 shrimp per tank and reared on the experimental diets for 50 days.After the feeding trial,the growth performance,survival,feed conversion rate (FCR),body composition and protease and lipase activities in the hepatopancreases of the experimental shrimps were determined.The results show that weight gain (WG),specific growth rate (SGR),FCR,body protein,body Lys and Arg content were significantly affected by dietary Lys and Arg (P<0.05) and improved when dietary Lys and Arg levels were 2.11%-2.51% and 1.80%-2.21%,respectively.Protease and lipase activities in the hepatopancreases of the shrimps appeared higher when dietary Lys and Arg quantities were 2.11%-2.51% and 1.80%-2.21%,although the difference was not statistically significant (P>0.05).Therefore,according to our results,the optimal Lys and Arg quantities in the diet of Pacific white shrimp,L.vannamei,were considered to be 2.11%-2.51% and 1.80%-2.21%,respectively,and the optimal ratio to be 1:0.88-1:1.05.

  10. Tratamiento de efluentes del cultivo de Litopenaeus vannamei por sedimentación y filtración por la ostra Crassostrea rhizophorae Treatment of Litopenaeus vannamei farming effluents by sedimentation and oyster Crassostrea rhizophorae filtration

    Directory of Open Access Journals (Sweden)

    Roberto Ramos

    2008-01-01

    Full Text Available A escala de laboratorio se evaluaron las eficiencias de remoción del material particulado y nutrientes disueltos desde efluentes de cultivo de Litopenaeus vannamei, utilizando un sistema combinado de dos fases, sedimentación y filtración por la ostra Crassostrea rhizophorae, empleando tres tiempos de retención hidráulica (TRH del efluente, 6, 12 y 24 h. En la fase de sedimentación, se emplearon estanques de 100 L en los cuales fueron colocados 90 L de efluente. En la fase de filtración, se utilizaron estanques de 50 L en los cuales se colocaron 20 L del efluente sobrenadante correspondiente a cada TRH de la fase de sedimentación. Cada unidad experimental de la fase de filtración fue sembrada con 15 ejemplares adultos de ostras con un peso promedio de 80 g manteniendo una biomasa de 1.200 g estanque-1 . Se utilizó un estanque control para cada TRH, conteniendo solamente conchas de ostras sin animal. Los resultados indicaron que en la fase de sedimentación el mejor desempeño fue alcanzado en el tiempo de 24 h, con 56,1% de remoción para la turbidez, 36,3% para los sólidos volátiles totales, la DB0(5 con 31,0% y el amonio con 21,4%. En la fase de filtración, la mayor eficiencia de remoción fue alcanzada en el tiempo de 6 h, con valores de 62,1% para la turbidez, 69,4% para los sólidos disueltos totales, 35,4% para los sólidos volátiles totales, 100% para la clorofila a y 17,2% para la DB0(5, en relación a los valores del estanque control. En esta fase, el amonio presentó un aumento en su concentración en todos los TRH. Calculadas las eficiencias de remoción con las dos fases integradas: sedimentación y filtración, en el tiempo de 6 h, en relación al efluente bruto, el desempeño mejoró significativamente alcanzando valores de 69,3% para la turbidez, 69,0% para los sólidos disueltos totales, 41,3% para los sólidos volátiles totales y 100% para la clorofila a.Efficiency in removing particulate matter and dissolved

  11. A hemocyte-expressed fibrinogen-related protein gene (LvFrep) from the shrimp Litopenaeus vannamei: Expression analysis after microbial infection and during larval development.

    Science.gov (United States)

    Coelho, Jaqueline da Rosa; Barreto, Cairé; Silveira, Amanda da Silva; Vieira, Graziela Cleusa; Rosa, Rafael Diego; Perazzolo, Luciane Maria

    2016-09-01

    Fibrinogen-related proteins (FREPs) comprise a large family of microbial recognition proteins involved in many biological functions in both vertebrate and invertebrate animals. By taking advantage of publicly accessible databases, we have identified a FREP-like homolog in the most cultivated penaeid shrimp, Litopenaeus vannamei (LvFrep). The obtained sequence showed a conserved fibrinogen-related domain (FReD) and displayed significant similarities to FREP-like proteins from other invertebrates and to ficolins from crustaceans. The expression of LvFrep appeared to be limited to circulating hemocytes. Interestingly, LvFrep gene expression was induced in shrimp hemocytes only in response to a Vibrio infection but not to the White spot syndrome virus (WSSV). Moreover, LvFrep transcript levels were detected early in fertilized eggs, suggesting the participation of this immune-related gene in the antimicrobial defenses during shrimp development. PMID:27380968

  12. A hemocyte-expressed fibrinogen-related protein gene (LvFrep) from the shrimp Litopenaeus vannamei: Expression analysis after microbial infection and during larval development.

    Science.gov (United States)

    Coelho, Jaqueline da Rosa; Barreto, Cairé; Silveira, Amanda da Silva; Vieira, Graziela Cleusa; Rosa, Rafael Diego; Perazzolo, Luciane Maria

    2016-09-01

    Fibrinogen-related proteins (FREPs) comprise a large family of microbial recognition proteins involved in many biological functions in both vertebrate and invertebrate animals. By taking advantage of publicly accessible databases, we have identified a FREP-like homolog in the most cultivated penaeid shrimp, Litopenaeus vannamei (LvFrep). The obtained sequence showed a conserved fibrinogen-related domain (FReD) and displayed significant similarities to FREP-like proteins from other invertebrates and to ficolins from crustaceans. The expression of LvFrep appeared to be limited to circulating hemocytes. Interestingly, LvFrep gene expression was induced in shrimp hemocytes only in response to a Vibrio infection but not to the White spot syndrome virus (WSSV). Moreover, LvFrep transcript levels were detected early in fertilized eggs, suggesting the participation of this immune-related gene in the antimicrobial defenses during shrimp development.

  13. Aspects moléculaires et biochimiques des stylicines, peptides multifonctionnels identifiés chez la crevette bleue du Pacifique Litopenaeus stylirostris (Crustacea, Decapoda)

    OpenAIRE

    Rolland, Jean-Luc

    2010-01-01

    Les travaux présentés dans ce mémoire ont été motivés par l’importance économique de l’élevage de la crevette bleue du pacifique Litopenaeus stylirostris dont les fortes mortalités sont principalement dues au développement de maladies bactériennes et virales. Ils ont consisté en la caractérisation des deux premiers membres d’une famille originale de peptides multifonctionnels présents chez les crevettes pénéides, les stylicines. Ces peptides, nommés stylicines 1 et 2, sont des peptides anioni...

  14. Les capacités osmorégulatrices chez la crevette bleue Litopenaeus stylirostris, au cours de l’ontogenèse

    OpenAIRE

    Pham, Dominique

    2011-01-01

    L’étude de l’ontogenèse de l’osmorégulation a été entreprise chez la crevette bleue Litopenaeus stylirostris en Nouvelle-Calédonie. L’implication des tissus de la cavité branchiale dans la régulation ionique a été déterminée au préalable chez des juvéniles par immunolocalisation de trois transporteurs protéiques d’ions, ( la NKA, le co-transporteur NKCC1 et le CFTR ) et par la détection de ionocytes en microscopie électronique. Le rôle de la NKA a été particulièrement étudié et son implicatio...

  15. Does damming of the Colorado River affect the nursery area of blue shrimp Litopenaeus stylirostris (Decapoda: Penaeidae) in the Upper Gulf of California?

    Science.gov (United States)

    Aragón-Noriega, E A; Calderón-Aguilera, L E

    2000-12-01

    After damming the Colorado River the freshwater flow was reduced to 1% of its virgin flow to the Upper Gulf of California (UGC). The ecological effects need to be properly documented. The UGC is the nursery area for Litopenaeus stylirostris, the most profitable fishery in the zone. In order to know the relative abundance of L. stylirostris postlarval stage we conducted a sampled survey every 14 days in 1993, 1994 and 1997, plus an intensive sampling during a complete tide cycle in July 1995 and 1996. We did 10 min trawls each hour during the flood tide. Relative abundance of postlarvae was higher (p < 0.05) in those years when freshwater flow reached the UGC. PMID:11487932

  16. First report on White Spot Syndrome Virus (WSSV infection in white leg shrimp Litopenaeus vannamei (Crustacea, Penaeidae under semi intensive culture condition in India

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    Gunalan Balakrishnan

    2011-07-01

    Full Text Available Scientific shrimp culture began in India in the late eighties along the east coast particularly inAndrapradesh and Tamilnadu. Continuous success of shrimp culture was affected by mass mortalities ofcultured shrimp in 1994. Thereafter disease infection on survival and production of shrimps get itsimportance in culture. The present study is the first report on WSSV (white spot syndrome virusinfection in cultured Litopenaeus vannamei (Boone, 1931 in India. WSSV infection was observed on 70thdays of culture due to cross contamination of white spot infected shrimp from the neighboring farmbecause of birds. Due to this infection within two days the mortality ratio has gone up to 25% in pond 1and 12% in pond 2. So this present study strongly recommends to every shrimp farmers to go for birdfencing & crab fencing to avoid horizontal contamination, before stocking the good quality seed, thenthey will have the risk free WSSV culture.

  17. INFLUÊNCIA DA REFRIGERAÇÃO NA PRESERVAÇÃO DO NÚMERO TOTAL DE HEMÓCITOS DE CAMARÕES MARINHOS Litopenaeus vannamei CULTIVADOS UTILIZANDO-SE CITRATO DE SÓDIO

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    Andréa Christianne Gomes Barretto

    2012-12-01

    Full Text Available For carrying out blood cell count, the use anti-coagulants is necessary, and in the specific case of the hemolymph in shrimp, there is still no standard solution to avoid its coagulation. Sodium citrate is frequently used as anti-coagulant, because it has been previously proven to be efficient in the preservation of the hemocytes of the sea shrimp Litopenaeus vannamei. In 140 samples of hemolymph with sodium citrate (10% in the ratio of 1:1, collected at 2 stages of cultivation, in dry and wet season, were examined for HTC immediately after collection and then again after storage in the refrigerator. All data were analyzed using modeling mathematics formulas (P < 0.05. After storage in the refrigerator, the number of cells decreased considerably. Therefore, the anticoagulant sodium citrate only preserved the hemolymph of the shrimp Litopenaeus vannamei for HTC when the count was done immediately after collection.

  18. Cross breeding of different domesticated lines as a simple way for genetic improvement in small aquaculture industries: Heterosis and inbreeding effects on growth and survival rates of the Pacific blue shrimp Penaeus (Litopenaeus) stylirostris

    OpenAIRE

    Goyard, Emmanuel; Goarant, Cyrille; Ansquer, Dominique; Brun, Pierre; De Decker, Sophie; Dufour, Robert; Galinie, C; Peignon, Jean-marie; Pham, Dominique; Vourey, Elodie; Harache, Yves; Patrois, Jacques

    2008-01-01

    Two populations of the Latin American shrimp Penaeus (Litopenaeus)stylirostris domesticated in Hawaii and in New Caledonia were previously shown to be genetically differentiated and proven highly inbred. In New Caledonia, where different Vibriosis affect shrimp production and antibiotic use is banned in growing ponds, the Hawaiian population was introduced to increase the allelic variability available for local shrimp farmers and start a genetic improvement program. Growth and survival rates ...

  19. Size-composition of Annual Landings in the White Shrimp, Litopenaeus setiferus, Fishery of the Northern Gulf of Mexico, 1960–2006: Its Trend and Relationships with Other Fishery-dependent Variable

    OpenAIRE

    Nance, James M.

    2010-01-01

    The potential for growth overfishing in the white shrimp, Litopenaeus setiferus, fishery of the northern Gulf of Mexico appears to have been of limited concern to Federal or state shrimp management entities, following the cataclysmic drop in white shrimp abundance in the 1940’s. As expected from surplus production theory, a decrease in size of shrimp in the annual landings accompanies increasing fishing effort, and can eventually reduce the value of the landings. Growth overfishing can exa...

  20. Effect synergistic of the radiation and of the refrigeration in the conservation of the white shrimp (Litopenaeus vannamei);Efeito sinergistico da radiacao gama e de refrigeracao na conservacao do camarao-branco-do-pacifico (Litopenaeus vannamei)

    Energy Technology Data Exchange (ETDEWEB)

    Guimaraes-Lopes, Tatiana Giselle

    2006-07-01

    Taking into account shrimp susceptibility to autolise and bacterial decomposition, this study aimed to evaluate the different gamma irradiation doses influence over meat quality related to microbiological, physical-chemical, nutritional and sensorial aspects, during cold storage of the white shrimp (Litopenaeus vannamei). Samples were taken from fresh shrimps without cephalothoraxes, settled into polystyrene trays, packed with flexible plastic film and submitted to different doses of gamma irradiation (1.0 and 3.5 kGy). Samples were kept under refrigeration (5 deg C) and analyzed at the 1st, 7th, 14th and 21st day of storage. Microbiological analyzes were realized (Coagulase-positive Staphylococcus, Salmonella ssp, coliforms, psychotropic and mesophilic population), and physical-chemical and tests (Total volatile bases (TVNB), pH, non proteic nitrogen (NNP), TBARS analysis - reactive substances to thiobarbituric acid (TBA), trimetil amine (TMA) cholesterol tenor and proximate composition). Instrumental color test were realized and hedonic test was taken to determine cooked products sensorial quality during storing period. It was noticed that the irradiation process reduced bacterial population and during storage all the irradiated samples were under the patterns established by the Brazilian legislation (Brazil, 2005a). Non-irradiated samples were inappropriate for consumption after one week of storage, since they were over Staphylococcus established limits. The pH values ranged from 6.05 to 6.39; 19.47 to 80.84 mg.100g-1 for TVNB; 2.61 to 12.85 mg.100g-1 for TMA-N; 0.22 a 1.20 mg of malonaldeide.1000g{sup -1} for TBA and 53.09 to 134.81 mg.100g-1 for cholesterol concentrations. Irradiated samples kept more stability during storage period when compared to non-irradiated samples, regarding to pH, TVNB and TMA levels. Samples irradiation resulted into cholesterol and TBA values reduction at different evaluated moments. No significant differences (p <= 0.05) were found

  1. Observações sobre calazar em Jacobina, Bahia. VI - Investigações sobre reservatórios silvestres e comensais

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    I. A. Sherlock

    1988-03-01

    Full Text Available Durante os anos de 1982 a 1986, a investigação sobre mamíferos comensais e silvestres, da periferia da cidade de Jacobina, Bahia, mostrou, ao lado do escasso número de exemplares, uma reduzida variedade específica dessa fauna. Capturou-se apenas 11 espécies, entre as quais, predominou o Didelphis albiventris, que abrangeu 44% dos 213 espécimens capturados. Entre os 193 com exames já concluídos, 84 eram exemplares de D. albiventris e 2 estavam infectados pela Leishmania donovani senso lato, 1 por L. mexicana amazonensis, 1 por L. braziliensis, subespécie e 3 por Trypanosoma cruzi Também foram observadas formas suspeitas de serem amastigotas de leishmanics, nos esfregaços de órgãos de 3 exemplares de Dasyprocta aguti, 1 Cercomys cunicularius - e 1 Oryzomys eliurus. 0 restante dos exemplares, inclusive 14 de Lycalopex vetulus, estava negativo para flagelados. Apesar de reforçado por outros indicadores epidemiológicos, como a predominância específica, a freqüência domiciliar, a atratividade para a vetora Lutzomyia longipalpis, e a concomitância com casos humanos nos mesmos locais, o índice de 2,3% de infecção natural do Didelphis albiventris, não autoriza a conclusão definitiva de ser o marsupial o mais importante reservatório natural da leishmaniose visceral em Jacobina.From 1982 to 1986, investigations on the natural infection with leishmanias of the sylvatic and commensal mammals were carried out around the city of Jacobina, Bahia, one of the oldest endemic foci of visceral leishmaniasis in Brazil. The species of Marsupialia Didelphis albiventris predominated, with the rate of 44%, over the total of 213 specimens belonging to only 11 different species of mammals collected there. Among the 84 specimens of D. albiventris examined, 2(2.3% were infected with Leishmania donovani sensu lato; 1 with L. mexicana amazonensis, 1 with L. braziliensis sub species, and 3 with Trypanosoma cruzi. Also, amastigote suspected bodies were

  2. Parásitos Gastrointestinales en Monos de Margarita Sapajus apella margaritae (Primates: Cebidae en Estado Silvestre (Nueva Esparta, Venezuela. Hallazgos preliminares -resumen-

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    Glencora M Montesinos-López

    2014-12-01

    Full Text Available El mono capuchino de Margarita es el único primate endémico del territorio insular venezolano y habita las áreas boscosas del oriente de la Isla de Margarita (Edo. Nueva Esparta. Se encuentra bajo la categoría ‘’En Peligro Crítico’’ en la Lista Roja de Especies Amenazadas (UICN y en el Apéndice II del CITES. La fragmentación del hábitat‚ la cacería como plaga de cultivos y el tráfico de monos como mascotas‚ son sus principales amenazas‚ todas de carácter antropogénico. Con el objetivo de evaluar el estado sanitario de esta especie‚ se llevó a cabo un muestreo fecal no invasivo de ejemplares silvestres para realizar descarte de parásitos gastrointestinales. Las muestras se recolectaron durante 2013 - 2014 en el cerro Matasiete (11‚4 Km2 (parte del Monumento Natural Cerros Matasiete y Guayamurí y en el Parque Nacional Cerro El Copey (71,3 km2. Se emplearon plataformas de madera‚ previamente cebadas mediante colocación recurrente de frutas. Se procedió a la recolección seriada de heces sobre las mismas‚ confirmando su origen a través de cámaras trampa y la observación directa de los monos alimentándose sobre estas superficies. Las muestras de heces se conservaron refrigeradas hasta su análisis en el Hospital Veterinario Salamanca‚ para ser procesadas mediante evaluación cualitativa (examen directo y método de concentración por flotación de Willis y técnica especial de cultivos larvarios en algunos casos. Se recolectaron un total de 35 muestras donde se reportó la presencia de huevos de nemátodos de los órdenes Strongylida (45%‚ n=16‚ Rhabditida (20%‚ n=7‚ Spirurida (14%‚ n=5 y Ascaridida (11%‚ n=4‚ identificados en cooperación con la Universidad Central de Venezuela. Estos resultados señalan por primera vez la naturaleza del parasitismo en esta población de primates‚ revelando la necesidad de estudios posteriores con énfasis en identificación más detallada de estos par

  3. Las abejas silvestres de Colombia: Por qué y cómo conservarlas The Colombian wild bees: Why and how to preserve them

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    González Víctor Hugo

    2000-06-01

    Full Text Available Las abejas silvestres de Colombia, al igual que las del resto del mundo, están sufriendo los rigores de las actividades desarrolladas por el hombre y así se catalogan como organismos en peligro. El conocimiento actual de las abejas en nuestro país, como muchos otros insectos, todavía se encuentra en una fase muy incipiente; es fragmentado, local y carece de una síntesis. Tan sólo se conoce racionalmente 5% de las abejas del país, especialmente las especies corbiculadas de la familia Apidae. Muy poco trabajo taxonómico ha sido realizado por investigadores colombianos y prácticamente no existen trabajos por autores extranjeros enfocados en la fauna colombiana. La carencia de especialistas, las dificultades para el envío de material al exterior y la falta de recursos han dificultado la tarea. Sin embargo, la alta riqueza de especies que creemos se encuentra en el país representa una razón más para avanzaren el conocimiento y entendimiento de la biodiversidad de éste grupo. En esta revisión se analizan las principales causas que ponen en riesgo las poblaciones y especies de la apifauna nativa: deforestación, pastoreo, la abeja africanizada y la explotación irracional de las abejas, entre otras. Se proponen algunas acciones a ser emprendidas conjuntamente con centros académicos, agricultores, campesinos, indígenas y la sociedad en general que permitan proteger las especies, teniendo en cuenta que median-te la polinización, se tornan en eslabones esenciales dentro de los ecosistemas permitiendo la conservación de muchas especies vegetales y otras comunidades.The Colombian wild bees, as well as those in the rest of the whole world are undergoing the rigor of human activities and so are considered to be under threat of extinction. The current knowledge about Colombian bees, like other insects, is still in its beginnings, is fragmentated and a synthesis is desirable. Only 5% of the Colombian bees are well known, especially the

  4. Coinfección entre Distemper Canino y un Verme Pulmonar en un Cerdocyon thous en Estado Silvestre en el Municipio de Pereira

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    Nestor Varela-Arias

    2014-12-01

    áticos; en pulmón neumonía broncointersticial con cuerpos de inclusión y múltiples microgranulomas con nemátodos  en estadío L1 intralesionales; en cerebro cambios hipóxicos y en riñón nefrosis tubular multifocal; entre otros hallazgos. La evidencia confirma como causa de muerte una falla multiorgánica por coinfección entre el virus del distemper (Morbillivirus ARN -‚ familia Paramyxoviridae que afecta diferentes mamíferos del orden CARNIVORA y vermes pulmonares compatibles con Filaroides osleri (Oslerus o Angiostrongylus sp. Ambos agentes causan muertes con impacto directo en las poblaciones de zorros en otras latitudes‚ sin embargo la interacción patogénica no está bien descrita‚ por lo que se recomienda monitorear las poblaciones silvestres de C. thous en Galicia‚ zona en la que se han presentado muertes de zorros con signos similares‚ además de importantes generadores de cambio ecosistémico (expansión urbana‚ construcción y operación de proyectos turísticos.

  5. Procedencia y uso de madera de pino silvestre y pino laricio en edificios históricos de Castilla y Andalucía

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    Rodríguez Trobajo, Eduardo

    2008-12-01

    Full Text Available The productive cycle of Scots pine (Pinus sylvestris L. and black pine (Pinus nigra Arn. within the medieval Castilian and Andalusian carpentry is here analysed. New criteria to identify both timbers and its dendrochronological date are shown as previous facts to determine their geographical origin. Historical names of these species and other names, such as alerce, with a vague allocation are also studied. Main historical areas of resource and river ways of Tajo and Guadalquivir used to transport the wood (black pine to interior cities (Sevilla, Toledo, Madrid … are thus identified. On the other hand, wood’s diversification and its selective use is analysed regarding its resistance value. Availability also determines that a sort of wood becomes fossil-guide (especie-guía, which is proposed as chronological indicator for several periods and constructive contexts. Some timber remains dating to the first millennium are studied in detail. They belong to the Mosque of Cordoba and four early medieval churches sited in the Duero valley (La Nave, Baños, Quintanilla and Barriosuso. The empiric dating and analysis of this material offer a post quem chronology for the building of these churches and new facts about the distribution of theses wood’s species in the north-western Iberian peninsula.Se realiza un recorrido a través del ciclo constructivo de las maderas de pino silvestre (Pinus sylvestris L. y pino laricio (Pinus nigra Arn. en el ámbito de la carpintería medieval de Castilla y Andalucía. Nuevos criterios para diferenciar las dos especies de madera y su datación dendrocronológica, son aportados como datos previos para determinar el origen geográfico del material. Son objeto de discusión los nombres históricos de estas especies y otras voces, como alerce, que tienen una imprecisa asignación. Se identifican así las principales áreas históricas de aprovechamiento y las vías fluviales del Tajo y Guadalquivir utilizadas para el

  6. Contribución al Conocimiento de los Parámetros Hematológicos en Armadillo Nueve Bandas (Dasypus novemcinctus, en Núcleos de Reubicación de Fauna Silvestre en la Orinoquia Colombiana (resumen

    Directory of Open Access Journals (Sweden)

    Juan S Melo-Restrepo

    2013-08-01

    Full Text Available El armadillo nueve bandas (Dasypus  novemcinctus, es un pequeño mamífero placentado de la familia Dasypodidae recubierto por una armadura de placas óseas cubiertas por escudos córneos, su distribución va desde Argentina y Chile en Latino América hasta el sur de los Estados Unidos, su carne es muy apetecida, por lo cual es grande la presión del hombre sobre la especie a través de la caza indiscriminada y la destrucción de los hábitats naturales. Con el propósito de conocer el comportamiento y la sanidad de la especie Ex situ se practicó un muestreo a cinco especímenes adultos en los núcleos de reubicación de fauna silvestre bajo el área de influencia de CORMACARENA.

  7. Characterization of rust, early and late leaf spot resistance in wild and cultivated peanut germplasm Caracterização da resistência à ferrugem, mancha preta e mancha castanha em germoplasma silvestre e cultivado de amendoim

    Directory of Open Access Journals (Sweden)

    Alessandra Pereira Fávero

    2009-02-01

    Full Text Available Groundnut (Arachis hypogaea has an AB genome and is one of the most important oil crops in the world. The main constraints of crop management in Brazil are fungal diseases. Several species of the genus Arachis are resistant to pests and diseases. The objective of our experiments was to identify wild species belonging to the taxonomic section Arachis with either A or B (or " non-A" genomes that are resistant to early leaf spot (Cercospora arachidicola, late leaf spot (Cercosporidium personatum and rust (Puccinia arachidis. For the identification of genotypes resistant to fungal diseases, bioassays with detached leaves were done in laboratory conditions, with artificial inoculation, a controlled temperature of 25ºC and a photoperiod of 10 h light/14 h dark, for 20-42 days, depending on the fungi species. Most of the accessions of wild species were more resistant than accessions of A. hypogaea for one, two or all three fungi species studied. Arachis monticola, considered to be a possible tetraploid ancestor or a derivative of A. hypogaea, was also more susceptible to Cercosporidium personatum and Puccinia arachidis, as compared to most of the wild species. Therefore, wild germplasm accessions of both genome types are available to be used for the introgression of resistance genes against three fungal diseases of peanut.O amendoim (Arachis hypogaea possui genoma AB e é uma das mais importantes culturas oleaginosas em todo o mundo. Os principais problemas da cultura no Brasil são as doenças fúngicas. Várias espécies do gênero Arachis são resistentes a pragas e doenças. Este trabalho visou a identificar espécies silvestres pertencentes à seção Arachis associadas aos genomas A ou B (ou " não-A" do amendoim que são resistentes à mancha castanha (Cercospora arachidicola, mancha preta (Cercosporidium personatum e ferrugem (Puccinia arachidis. Para a identificação de genótipos resistentes a doenças fúngicas, bioensaios utilizando

  8. Implicación epidemiológica de carnívoros silvestres de ambientes atlánticos en el mantenimiento y dispersión de Escherichia coli, Trichinella SPP y Ectoparásitos

    OpenAIRE

    Díaz Alcázar, Eduardo Alfonso

    2016-01-01

    El aumento de la población humana está provocando la invasión de diferentes ecosistemas y, con ello, un mayor contacto entre la fauna silvestre, doméstica y el hombre. Algunas especies de carnívoros generalistas han sido capaces de adaptarse con éxito a los entornos antropogénicos, incrementando sus poblaciones; el hecho de que estén situadas en lo alto de la cadena trófica, que sean más longevas y con mayor capacidad de dispersión que otras especies, hace que estos carnívoros actúen como bio...

  9. Effect synergistic of the radiation and of the refrigeration in the conservation of the white shrimp (Litopenaeus vannamei)

    International Nuclear Information System (INIS)

    Taking into account shrimp susceptibility to autolise and bacterial decomposition, this study aimed to evaluate the different gamma irradiation doses influence over meat quality related to microbiological, physical-chemical, nutritional and sensorial aspects, during cold storage of the white shrimp (Litopenaeus vannamei). Samples were taken from fresh shrimps without cephalothoraxes, settled into polystyrene trays, packed with flexible plastic film and submitted to different doses of gamma irradiation (1.0 and 3.5 kGy). Samples were kept under refrigeration (5 deg C) and analyzed at the 1st, 7th, 14th and 21st day of storage. Microbiological analyzes were realized (Coagulase-positive Staphylococcus, Salmonella ssp, coliforms, psychotropic and mesophilic population), and physical-chemical and tests (Total volatile bases (TVNB), pH, non proteic nitrogen (NNP), TBARS analysis - reactive substances to thiobarbituric acid (TBA), trimetil amine (TMA) cholesterol tenor and proximate composition). Instrumental color test were realized and hedonic test was taken to determine cooked products sensorial quality during storing period. It was noticed that the irradiation process reduced bacterial population and during storage all the irradiated samples were under the patterns established by the Brazilian legislation (Brazil, 2005a). Non-irradiated samples were inappropriate for consumption after one week of storage, since they were over Staphylococcus established limits. The pH values ranged from 6.05 to 6.39; 19.47 to 80.84 mg.100g-1 for TVNB; 2.61 to 12.85 mg.100g-1 for TMA-N; 0.22 a 1.20 mg of malonaldeide.1000g-1 for TBA and 53.09 to 134.81 mg.100g-1 for cholesterol concentrations. Irradiated samples kept more stability during storage period when compared to non-irradiated samples, regarding to pH, TVNB and TMA levels. Samples irradiation resulted into cholesterol and TBA values reduction at different evaluated moments. No significant differences (p <= 0.05) were found in

  10. Experimental infection with different bacterial strains in larvae and juvenile Litopenaeus vannamei reared in Santa Catarina State, Brazil - doi: 10.4025/actascibiolsci.v32i3.5471 Experimental infection with different bacterial strains in larvae and juvenile Litopenaeus vannamei reared in Santa Catarina State, Brazil - doi: 10.4025/actascibiolsci.v32i3.5471

    Directory of Open Access Journals (Sweden)

    Adolfo Jatoba

    2010-09-01

    Full Text Available This study evaluated the pathogenic characteristics of bacteria isolated from Litopenaeus vannamei during an outbreak at the Laboratory of Marine Shrimp, UFSC, Santa Catarina State, Brazil. Their virulence potential in larvae and juvenile shrimp and the effects on the total haemocyte count, phenoloxidase activity and serum agglutinate titre were examined after experimental infection. Bacterial strains were isolated from larvae and adult shrimps, identified by the AP120E biochemical system as: two strains of Vibrio alginolyticus, three of Aeromonas salmonicida and one of Pasteurella multocida sp. and Pasteurella sp. All the bacterial strains isolated in this study caused mortality in shrimp. One strain of V. alginolyticus was responsible for 97.3 and 88.7% mortality in larvae and juvenil shrimps, respectively. The shrimp immunological system was influenced by experimental infection with V. alginolyticus. Decrease in the total haemocyte count and increase in the phenoloxidase activity and the serum agglutinate titre (p V. alginolyticus isolated from larvae and juvenile reared marine shrimp.This study evaluated the pathogenic characteristics of bacteria isolated from Litopenaeus vannamei during an outbreak at the Laboratory of Marine Shrimp, UFSC, Santa Catarina State, Brazil. Their virulence potential in larvae and juvenile shrimp and the effects on the total haemocyte count, phenoloxidase activity and serum agglutinate titre were examined after experimental infection. Bacterial strains were isolated from larvae and adult shrimps, identified by the AP120E biochemical system as: two strains of Vibrio alginolyticus, three of Aeromonas salmonicida and one of Pasteurella multocida sp. and Pasteurella sp. All the bacterial strains isolated in this study caused mortality in shrimp. One strain of V. alginolyticus was responsible for 97.3 and 88.7% mortality in larvae and juvenil shrimps, respectively. The shrimp immunological system was influenced by

  11. Assessing the potential toxicity of marine sediments found in petroleum industry areas: A new approach based on responses of postlarval shrimp; Evaluacion del potencial de toxicidad de sedimentos marinos en areas de la industria petrolera: Un nuevo metodo basado en respuestas de postlarvas de camarones

    Energy Technology Data Exchange (ETDEWEB)

    Evangelista, A.J.A.; Nascimento, I.A.; Pereira, S.A.; Lopes, M.B.N.L.; Martins, L.K.P. [Laboratorio de Bio Marinha e Biomonitoramento IBIO-UFBA, Campus Universitario de Ondina, Salvador (Brazil); Fillmann, G [Plymouth Marine Laboratory, West Hoe, PL (United Kingdom)

    2005-03-15

    In this study we tested the toxicity of bulk sediment from the northeastern area of Todos os Santos Bay, Bahia, Brazil, to evaluate environmental impact induced by 50 years of exposure to the local petroleum industry (Petrobras). Sediment samples were collected during one year, at three-month intervals, from four sites in areas of oil extraction (Ilha das Fontes, station 4), transportation (Ilhas de Madre de Deus and Pati, stations 2 and 3) and refinement (RELAM, station1). Two reference stations (5 and 6) were located outside the petroleum influence area, to the south of the bay. Static bioassays were conducted for 96 h, using 7-8 day old Litopenaeus vannamei postlarvae (PL). The assays were conducted in 2.5 L plastic jars containing 200 g of surface (1 cm deep) bulk sediment covered by 2 L of dilution water (filtered seawater, 28 ppt salinity, 27 + 2 degree centigrade and DO under saturation). Fifteen exposed PL in each jar were fed daily on 60 recently hatched Artemia salina nauplii. Physico-chemical parameters were monitored. Mortality and dry weight gain were taken as end-points. The PL mortality data obtained for sediment from the Petrobras stations in comparison to the data from the reference stations were not significantly different (P > 0.05); however, the dry weight gain showed significant differences among stations. A maximum value was reached at station 5 (reference area) and a minimum at station 1 (RELAM refinery). Stations 2 and 3 in petroleum transportation areas did not show significant differences (P > 0.05). To evaluate the sensitivity of this bulk-sediment test in detecting contaminant effects generated by the petroleum industry, the toxicity data were considered in terms of the hydrocarbon levels analyzed in sediments from the same Petrobras areas and in one of the control areas, located outside the bay. The results support the assumption that the bulk-sediment bioassay on penaeid PL is a suitable methodology not only to distinguish between

  12. Evaluación del crecimiento y supervivencia en larvas de camarón blanco Litopenaeus vannamei usando como fuente de alimento microalgas vivas y congeladas

    Directory of Open Access Journals (Sweden)

    Elifonso Isiordia Pérez:

    2007-05-01

    Full Text Available Resumen matraces de 1.5 litros de capacidad con 5 El objetivo del presente rabajo fue evaluar el eplicas cada tratamiento. La densidad de efecto de una dieta monoalgal a base de dos microalgas a suministrar fue de 50,000 células presentaciones Tetraselmis sp. (vivas y / mililitro. Conforme mudaban de estadio se congeladas:producto comercial: INLAND hacía conteo de organismos vivos y se media Seafarm® en la supervivencia y crecimiento longitud El mayor porcentaje de supervivencia en larvas de camarón blanco Litopenaeus y crecimiento se registró en las larvas vannamei en estadios larvales desde limentadas con Tetraselmis sp. vivas (97.3 Protozoea 1 (PZ1 a Protozoea 3 (PZ3. El ±4.6 en PZI, 94.1 ± 2.13 en PZ2 y 30.7±12.6 experimento se realizó en las instalaciones del en PZ3. Sin embargo este resultado no es Centro de Investigación en Alimentación y comparable a los obtenidos con otras especies Desarrollo (CIAD, Unidad Mazatlán Sinaloa. El de microalgas como Chaetoceros muelleri e estudio se realizó bajo condiciones controladas Isochrysis galbana. de temperatura (28°C, salinidad (35‰ y fotoperiodo (12 h luz / oscuridad. Se sembraron 150 organismos en nauplio V en

  13. EFEITO DAS ALTAS DENSIDADES DE ESTOCAGEM NO CRESCIMENTO E SOBREVIVÊNCIA DE Litopenaeus vannamei NA FASE FINAL DE ENGORDA, CULTIVADOS EM SISTEMAS DE BIOFLOCOS (BFT

    Directory of Open Access Journals (Sweden)

    Adriana Ferreira Silva

    2013-09-01

    Full Text Available The aim of this study was to evaluate the effect of high stocking densities on survival, growth and feed conversion rates of Litopenaeus vannamei shrimp, in final growout phase, in a Biofloc Technology (BFT culture system, keeping the same water parameters for all treatments. Shrimps (11.96 ± 1.14 g were stocked in microcosms (0.50/m2 tanks, connected to a BFT system raceway. The study was carried out for 45 days. The shrimp were stocked at densities of 150, 300, 450 and 600 shrimp/m2. Bioflocs were collected for analysis of proximate composition. The results were submitted to one-way ANOVA, and differences were compared by Tukey test (α = 0.05. In T300 and T450, growth and survival were not affected by high stocking densities. The highest biomass reached (T450 was 5.1kg/m² and the best feed conversion rate was 1.54 in T150. The results of this study indicate that stocking densities in the proposed system can be high, but not exceeding 450 shrimp/m². Furthermore, even maintaining the same water parameters for all treatments, there was a negative effect between density and shrimp growth, confirming that this effect is behavioral.

  14. Use of oxygen consumption and ammonium excretion to evaluate the sublethal toxicity of cadmium and zinc on Litopenaeus schmitti (Burkenroad, 1936, Crustacea).

    Science.gov (United States)

    Barbieri, Edison

    2007-06-01

    Penaeid shrimps are important resources for worldwide fisheries and aquaculture. In Brazil, Litopenaeus schmitti (L. schmitti) is a important commercially exploited species and is an ideal animal for studying the impairment caused by the effects of heavy metals that are often detected in coastal areas. The main purpose of the present study was to detect the acute toxicity of cadmium and zinc to L. schmitti and investigate their effects on oxygen consumption and ammonium excretion, investigations that have not been carried out in this species before. First, the acute toxicity of cadmium and zinc to L. schmitti 24, 48, 72, and 96-hour medium lethal concentration was examined, which resulted in the following values: 0.98, 0.54, 0.32, and 0.18 mg/L for cadmium and 1.64, 1.22, 0.86, and 0.31 mg/L for zinc. Furthermore, we also found that exposure of shrimp to cadmium and zinc caused an inhibition in oxygen consumption of 55.92 and 44.09%, respectively, relative to the control. However, after separate exposure to cadmium and zinc, elevations in ammonium excretion were obtained, which were 174.28 and 162.5% higher than the control, respectively.

  15. First report of the pituitary adenylate cyclase activating polypeptide (PACAP) in crustaceans: conservation of its functions as growth promoting factor and immunomodulator in the white shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Lugo, Juana María; Carpio, Yamila; Morales, Reynold; Rodríguez-Ramos, Tania; Ramos, Laida; Estrada, Mario Pablo

    2013-12-01

    The high conservation of the pituitary adenylate cyclase activating polypeptide (PACAP) sequence indicates that this peptide fulfills important biological functions in a broad spectrum of organisms. However, in invertebrates, little is known about its presence and its functions remain unclear. Up to now, in non-mammalian vertebrates, the majority of studies on PACAP have focused mainly on the localization, cloning and structural evolution of this peptide. As yet, little is known about its biological functions as growth factor and immunomodulator in lower vertebrates. Recently, we have shown that PACAP, apart from its neuroendocrine role, influences immune functions in larval and juvenile fish. In this work, we isolated for the first time the cDNA encoding the mature PACAP from a crustacean species, the white shrimp Litopenaeus vannamei, corroborating its high degree of sequence conservation, when compared to sequences reported from tunicates to mammalian vertebrates. Based on this, we have evaluated the effects of purified recombinant Clarias gariepinus PACAP administrated by immersion baths on white shrimp growth and immunity. We demonstrated that PACAP improves hemocyte count, superoxide dismutase, lectins and nitric oxide synthase derived metabolites in treated shrimp related with an increase in total protein concentration and growth performance. From our results, PACAP acts as a regulator of shrimp growth and immunity, suggesting that in crustaceans, as in vertebrate organisms, PACAP is an important molecule shared by both the endocrine and the immune systems.

  16. Quantitation of infectious myonecrosis virus in different tissues of naturally infected Pacific white shrimp, Litopenaeus vannamei, using real-time PCR with SYBR Green chemistry.

    Science.gov (United States)

    da Silva, Suzianny Maria Bezerra Cabral; Pinheiro, Ana Cristina de Aguiar Saldanha; Coimbra, Maria Raquel Moura

    2011-11-01

    The Pacific white shrimp, Litopenaeus vannamei, is the most important shrimp species in volume in world aquaculture. However, in recent decades, outbreaks of diseases, especially viral diseases, have led to significant economic losses, threatening the sustainability of shrimp farming worldwide. In 2004, Brazilian shrimp farming was seriously affected by a new disease caused by the Infectious myonecrosis virus (IMNV). Thus, disease control based on rapid and sensitive pathogen detection methods has become a priority. In this study, a specific quantitation method for IMNV was developed using real-time PCR with SYBR Green chemistry and viral load of the principal target tissues of chronically infected animals was quantified. The quantitative analysis revealed that mean viral load ranged from 5.08×10(8) to 1.33×10(6)copies/μg of total RNA in the hemolymph, 5.096×10(5) to 1.26×10(3)copies/μg in the pleopods, 6.85×10(8) to 3.09×10(4)copies/μg in muscle and 8.15×10(6) to 3.90×10(3)copies/μg in gills. Different viral loads of IMNV were found with greater values in the hemolymph and muscle, followed by the pleopods and gills. PMID:21854806

  17. Effect of addition of Navicula sp. on plankton composition and postlarvae growth of Litopenaeus vannamei reared in culture tanks with zero water exchange

    Directory of Open Access Journals (Sweden)

    Yllana Ferreira-Marinho

    2014-07-01

    Full Text Available The aim of this study was to evaluate the effect of the addition of Navicula sp. on plankton composition and postlarvae growth of Litopenaeus vannamei reared in culture tanks with zero water exchange systems. Four treatments were considered: zero water exchange (ZWE; ZWE with the addition of feed (ZWE-F; ZWE with the addition of Navicula sp. (ZWE-N and ZWE with the addition of feed and Navicula sp. (ZWE-FN, all in triplicate. Shrimp of 17.7 ± 0.02 mg were stocked at a density of 2500 shrimp m-3 and microalgae added on the 1st, 5th and 15th day at a density of 5x10(4 cell mL-1. The shrimp were fed a commercial feed composed by 42% crude protein four times a day except in the ZWE treatment. For data analysis we used Cochran, Shapiro-Wilk, ANOVA, Tukey and Student-t tests (P < 0.05. The most frequent genera were: Anabaena, Arcella, Asplanchma, Bosmina, Brachionus, Cylindrotheca, Daphnia, Fragilaria, Hemiaulus, Keratella, Orthoseira, Oscillatoria, Phymatodocis, Rhabdonema, Skeletonema, Sckizothrix and Ulothrix. Significant differences between treatments were observed for TAN, NO2-N, alkalinity, final weight, weight gain, final biomass, biomass gain, feed conversion ratio, specific growth rate and survival. The ZWE-FN treatment showed better production parameters, indicating the benefits of the addition of Navicula sp. as a natural food source for L. vannamei postlarvae in zero water exchange systems.

  18. Functional Feed Assessment on Litopenaeus vannamei Using 100% Fish Meal Replacement by Soybean Meal, High Levels of Complex Carbohydrates and Bacillus Probiotic Strains

    Directory of Open Access Journals (Sweden)

    Rosalia Contreras

    2011-06-01

    Full Text Available Functional feed supplemented with alternative-economic nutrient sources (protein, carbohydrates, lipids and probiotics are being considered in shrimp/fish aquaculture production systems as an option to increase yield and profits and to reduce water pollution. In this study the probiotic potential to formulate functional feeds have been evaluated using four dietary treatments: Treatment 1 (B + Bs; Bacillus subtilis potential probiotic strain was supplemented to a soybeanmeal (SBM—carbohydrates (CHO basal feed. Treatment 2 (B + Bm; Bacillus megaterium potential probiotic strain was supplemented to the same SBM-CHO basal feed. In Treatment 3 (B; SBM-CHO basal feed was not supplemented with probiotic strains. Treatment 4 (C; fishmeal commercial feed (FM was utilized as positive control. Feeding trials evaluated the survival, growth, and food conversion ratio and stress tolerance of juvenile Litopenaeus vannamei (Boone Pacific white shrimp. Best overall shrimp performance was observed for animals fed with Treatment 1 (B+Bs; additionally, stress tolerance and hemolymph metabolites also showed the best performance in this treatment. SBM-CHO basal feed not supplemented with probiotic strains (B presented smaller growth and lower feed conversion ratio (FCR. Shrimps fed with the fishmeal commercial feed (C presented the lowest stress tolerance to high ammonia and low oxygen levels. Specifically selected B. subtilis strains are recommended to formulate functional and economical feeds containing high levels of vegetable; protein and carbohydrates as main dietary sources in L. vannamei cultures.

  19. Comparison of immune response in Pacific white shrimp, Litopenaeus vannamei, after knock down of Toll and IMD gene in vivo.

    Science.gov (United States)

    Liu, Yongjie; Song, Lei; Sun, Yuhang; Liu, Tao; Hou, Fujun; Liu, Xiaolin

    2016-07-01

    The Toll and immune deficiency (IMD) pathways are essential for inducing immune related genes during invasion of pathogens. In the present study, transcripts of eight pathway-related genes in Litopenaeus vannamei, including Toll, IMD, Pelle, IAP1, TRAF6, ALF, Crustin and Penaeidin3 were analyzed to further understand the potential relationship between Toll and IMD pathway. The high transcription levels of TRAF6, Pelle, Toll, IMD and IAP1 in selected tissues indicates their functional roles in Toll and IMD pathways. The increased mRNA expression of Toll and IMD detected in the early stage might suggest the inducible role of Toll and IMD upon bacterial infection. Moreover, the continuous increase of IMD and the high level of Pelle and TRAF6 in Vibrio anguillarum challenged group indicated that Gram-negative bacterium can activate both the Toll and IMD signaling pathway. Silencing of Toll by a dsRNA-mediated RNAi strongly increased the transcripts of IMD, Pelle, TRAF6, IAP1 and Akirin, knocking down of IMD also markedly increased the transcripts of Toll, Pelle, IAP1 and Akirin. Furthermore, ALF expression was significantly increased in response to V. anguillarum and Micrococcus lysodeikticus challenge, while the transcripts of Crustin and Pen3 in hemocytes were significantly reduced in V. anguillarum group, but rose significantly following M. lysodeikticus infection. In summary, we speculate that Toll and IMD pathway are not independent in shrimp, but linked to defense against bacterial infection. PMID:26855014

  20. Dynamics of vitellogenin and vitellogenesis-inhibiting hormone levels in adult and subadult whiteleg shrimp, Litopenaeus vannamei: relation to molting and eyestalk ablation.

    Science.gov (United States)

    Kang, Bong Jung; Okutsu, Tomoyuki; Tsutsui, Naoaki; Shinji, Junpei; Bae, Sun-Hye; Wilder, Marcy N

    2014-01-01

    Levels of vitellogenin (VG) and vitellogenesis-inhibiting hormone (VIH) in the whiteleg shrimp, Litopenaeus vannamei, were measured by time-resolved fluoroimmunoassay in relation to the molting cycle and ovarian maturation induced by eyestalk ablation. During the molt cycle, VG mRNA expression levels and VG concentrations showed similar patterns of fluctuation. VG levels increased significantly at early intermolt (stage C0) in adults, but not in subadults. Unilateral and bilateral eyestalk ablation increased VG levels in adults, whereas only bilateral eyestalk ablation affected subadults. VIH levels showed contrasting patterns between adults and subadults. In adults, levels were high in late postmolt adults (stage B) and then low thereafter, whereas they increased from postmolt (stage A) to intermolt (stage C0) in subadults and remained high. Unilateral eyestalk ablation increased VIH levels 10 days following ablation in adults, after which levels decreased at 20 days. VIH levels decreased from 10 to 20 days after bilateral ablation. Both unilateral and bilateral ablation led to increased VIH levels in subadults. Eyestalk ablation induced ovarian maturation, but did not reduce VIH concentrations in the hemolymph. This phenomenon was perhaps due to other crustacean hyperglycemic hormone peptides having cross-reactivity with VIH antibodies. This is the first report to quantify concentrations of VG and VIH together in L. vannamei hemolymph, and to examine their relative dynamics.

  1. A Study on the Meat and Bone Meal and Poultry By-product Meal as Protein Substitutes of Fish Meal in Practical Diets for Litopenaeus vannamei Juveniles

    Institute of Scientific and Technical Information of China (English)

    ZHU Wei; MAI Kangsen; ZHANG Baigang; WANG Fuzhen; YU Yu

    2004-01-01

    A study was conducted to evaluate the effects of meat and bone meal(MBM)and poultry by-product meal(PBM)as the replacement of fish meal in the diets on the growth performance, survival and apparent digestibility coefficients(ADC)of Litopenaeus vannamei. The basal diets were formulated with 22% fish meal and other ingredients which provided about 40% protein and 9% lipid in the diet. The experimental diets included MBM or PBM to replace 0, 20%, 40%, 60% and 80% of total fish meal respectively. All diets were iso-nitrogenous and isocaloric in gross terms. The results showed that there were no significant differences(P>0.05)in growth performance and ADC among the treatments fed with the diets in which 0-60% fish meal had been replaced with MBM, while the percent weight gain(WG,%), body length gain(BLG,%)and ADC significantly decreased when the MBM was up to 80% of the fish meal. There were no significant differences(P>0.05)in growth performance and ADC among all the treatments fed with the diets in which 0-80% fish meal had been replaced with PBM.

  2. Quantitation of infectious myonecrosis virus in different tissues of naturally infected Pacific white shrimp, Litopenaeus vannamei, using real-time PCR with SYBR Green chemistry.

    Science.gov (United States)

    da Silva, Suzianny Maria Bezerra Cabral; Pinheiro, Ana Cristina de Aguiar Saldanha; Coimbra, Maria Raquel Moura

    2011-11-01

    The Pacific white shrimp, Litopenaeus vannamei, is the most important shrimp species in volume in world aquaculture. However, in recent decades, outbreaks of diseases, especially viral diseases, have led to significant economic losses, threatening the sustainability of shrimp farming worldwide. In 2004, Brazilian shrimp farming was seriously affected by a new disease caused by the Infectious myonecrosis virus (IMNV). Thus, disease control based on rapid and sensitive pathogen detection methods has become a priority. In this study, a specific quantitation method for IMNV was developed using real-time PCR with SYBR Green chemistry and viral load of the principal target tissues of chronically infected animals was quantified. The quantitative analysis revealed that mean viral load ranged from 5.08×10(8) to 1.33×10(6)copies/μg of total RNA in the hemolymph, 5.096×10(5) to 1.26×10(3)copies/μg in the pleopods, 6.85×10(8) to 3.09×10(4)copies/μg in muscle and 8.15×10(6) to 3.90×10(3)copies/μg in gills. Different viral loads of IMNV were found with greater values in the hemolymph and muscle, followed by the pleopods and gills.

  3. RNA-Seq reveals the dynamic and diverse features of digestive enzymes during early development of Pacific white shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Wei, Jiankai; Zhang, Xiaojun; Yu, Yang; Li, Fuhua; Xiang, Jianhai

    2014-09-01

    The Pacific white shrimp (Litopenaeus vannamei), with high commercial value, has a typical metamorphosis pattern by going through embryo, nauplius, zoea, mysis and postlarvae during early development. Its diets change continually in this period, and a high mortality of larvae also occurs in this period. Since there is a close relationship between diets and digestive enzymes, a comprehensive investigation about the types and expression patterns of all digestive enzyme genes during early development of L. vannamei is of considerable significance for shrimp diets and larvae culture. Using RNA-Seq data, the types and expression characteristics of the digestive enzyme genes were analyzed during five different development stages (embryo, nauplius, zoea, mysis and postlarvae) in L. vannamei. Among the obtained 66,815 unigenes, 296 were annotated as 16 different digestive enzymes including five types of carbohydrase, seven types of peptidase and four types of lipase. Such a diverse suite of enzymes illustrated the capacity of L. vannamei to exploit varied diets to fit their nutritional requirements. The analysis of their dynamic expression patterns during development also indicated the importance of transcriptional regulation to adapt to the diet transition. Our study revealed the diverse and dynamic features of digestive enzymes during early development of L. vannamei. These results would provide support to better understand the physiological changes during diet transition.

  4. 贮藏过程中凡纳滨对虾生物胺的变化%Changes of biogenic amines in Litopenaeus vannamei during storage

    Institute of Scientific and Technical Information of China (English)

    徐杰; 薛长湖; 赵庆喜; 李兆杰; 薛勇

    2007-01-01

    以邻苯二甲醛(OPA)为柱后衍生化试剂,采用反相HPLC法测定凡纳滨对虾(Litopenaeus vannamei)中的酪胺(Tyr)、腐胺(Put)、尸胺(Cad)、组胺(His)、胍丁胺(Agm)、精胺(Spe)和亚精胺(Spd)等7种生物胺.结合虾的感官分析、pH值和T-VBN值测定等方法评价凡纳滨对虾的品质,分析了凡纳滨对虾在不同贮藏温度和贮藏时间下的生物胺种类及含量的变化.结果表明,在0和4℃两种贮藏温度下,尸胺和腐胺发生的变化均最显著,可以其作为凡纳滨对虾新鲜程度的参考指标,确定最长贮藏时间分别为8 d和6 d.

  5. Innate immune-stimulating and immune genes up-regulating activities of three types of alginate from Sargassum siliquosum in Pacific white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Yudiati, Ervia; Isnansetyo, Alim; Murwantoko; Ayuningtyas; Triyanto; Handayani, Christina Retna

    2016-07-01

    The Total Haemocyte Count (THC), phenoloxidase (PO), Superoxide Dismutase (SOD) activity, Phagocytic Activity/Index and Total Protein Plasma (TPP) were examined after feeding the white shrimp Litopenaeus vannamei with diets supplemented with three different types of alginates (acid, calcium and sodium alginates). Immune-related genes expression was evaluated by quantitative Real Time PCR (qRT-PCR). Results indicated that the immune parameters directly increased according to the doses of alginates and time. The 2.0 g kg(-1) of acid and sodium alginate treatments were gave better results. Four immune-related genes expression i.e. LGBP, Toll, Lectin, proPO were up regulated. It is therefore concluded that the supplementation of alginate of Sargassum siliquosum on the diet of L. vannamei enhanced the innate immunity as well as the expression of immune-related genes. It is the first report on the simultaneous evaluation of three alginate types to enhance innate immune parameters and immune-related genes expression in L. vannamei. PMID:26993614

  6. Effect of Dietary Potassium on Growth, Nitrogen Metabolism, Osmoregulation and Immunity of Pacific White Shrimp (Litopenaeus vannamei) Reared in Low Salinity Seawater

    Institute of Scientific and Technical Information of China (English)

    LIU Hongyu; ZHANG Xinjie; TAN Beiping; LIN Yingbo; CHI Shuyan; DONG Xiaohui; YANG Qihui

    2014-01-01

    An 8 weeks feeding experiment was conducted to determine the effect of dietary potassium on the growth and physio-logical acclimation of Pacific white shrimp (Litopenaeus vannamei) reared in diluted seawater (salinity 4). Six semi-purified practical diets containing 0.59, 0.96, 1.26, 1.48, 1.74, and 2.17 g potassium K+per 100 g diet were formulated, respectively. The survival and feed conversion rate did not show significant difference among groups of shrimps given these diets (P>0.05). The shrimps fed the diets containing 0.96-1.48 g K+ per 100 g diet gained the highest weight, specific growth rate, and protein efficiency ratio. Their ammonium-N excretion rate as well as hemolymph concentration of Na+and Cl-were significantly lower than those of the control (P<0.05), but a reverse trend was observed for their gill Na+/K+-ATPase. Moreover, the shrimps fed with 1.48 g K+ per 100 g diet were the highest in hemolymph urea level, and the phenoloxidase and lysozyme activities were significantly higher than those of the control (P<0.05). The growth and physiological response of the test shrimps suggested that diet containing 1.48 g K+per 100 g diet improved the growth of L. vannamei in low-salinity seawater, and enhanced the physiological acclimation of the organism.

  7. The effects of feeding commercial feed formulated for semi-intensive systems on Litopenaeus vannamei production and its profitability in a hyper-intensive biofloc-dominated system

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    André Braga

    2016-05-01

    Full Text Available The present study evaluated the production of Litopenaeus vannamei in a high density biofloc-dominated system using two commercial feeds; a less expensive feed (US$0.99 kg−1 formulated for semi-intensive systems and the more expensive (US$1.75 kg−1 which was designed for hyper-intensive systems. A 67-days study was conducted in six 40 m3 lined with Ethylene Propylene Diene Monomer membrane raceways (RWs filled with mixture of seawater (22 m3, and biofloc-rich water (18 m3. Each RW was stocked (500 shrimp m−3 using juveniles (2.66 g produced from Taura-Resistant and Fast-Growth breeding lines. The study was composed of two feed treatments with three replicates each; the cheaper feed (SI-35 contained 35% crude protein (CP, 7% lipid and 4% fiber while the more expensive one (HI-35 had the same levels of CP and lipid but only 2% fiber. The SI-35 treatment required more solids removal, oxygen, and bicarbonate supplementation than the HI-35 treatment. Weekly growth, total biomass, yield were significantly lower in the SI-35 treatment, whereas feed conversion ratio was higher. The economic analysis indicates that both feeds would be commercially viable, nevertheless, the less-expensive feed financially underperformed the other.

  8. Correlation between Detection of a Plasmid and High-Level Virulence of Vibrio nigripulchritudo, a Pathogen of the Shrimp Litopenaeus stylirostris▿

    Science.gov (United States)

    Reynaud, Yann; Saulnier, Denis; Mazel, Didier; Goarant, Cyrille; Le Roux, Frédérique

    2008-01-01

    Vibrio nigripulchritudo, the etiological agent of Litopenaeus stylirostris summer syndrome, is responsible for mass mortalities of shrimp in New Caledonia. Epidemiological studies led to the suggestion that this disease is caused by an emergent group of pathogenic strains. Genomic subtractive hybridization was carried out between two isolates exhibiting low and high virulence. Our subtraction library was constituted of 521 specific fragments; 55 of these were detected in all virulent isolates from our collection (n = 32), and 13 were detected only in the isolates demonstrating the highest pathogenicity (n = 19), suggesting that they could be used as genetic markers for high virulence capacity. Interestingly, 10 of these markers are carried by a replicon of 11.2 kbp that contains sequences highly similar to those of a plasmid detected in Vibrio shilonii, a coral pathogen. The detection of this plasmid was correlated with the highest pathogenicity status of the isolates from our collection. The origin and consequence of this plasmid acquisition are discussed. PMID:18359828

  9. Correlation between detection of a plasmid and high-level virulence of Vibrio nigripulchritudo, a pathogen of the shrimp Litopenaeus stylirostris.

    Science.gov (United States)

    Reynaud, Yann; Saulnier, Denis; Mazel, Didier; Goarant, Cyrille; Le Roux, Frédérique

    2008-05-01

    Vibrio nigripulchritudo, the etiological agent of Litopenaeus stylirostris summer syndrome, is responsible for mass mortalities of shrimp in New Caledonia. Epidemiological studies led to the suggestion that this disease is caused by an emergent group of pathogenic strains. Genomic subtractive hybridization was carried out between two isolates exhibiting low and high virulence. Our subtraction library was constituted of 521 specific fragments; 55 of these were detected in all virulent isolates from our collection (n = 32), and 13 were detected only in the isolates demonstrating the highest pathogenicity (n = 19), suggesting that they could be used as genetic markers for high virulence capacity. Interestingly, 10 of these markers are carried by a replicon of 11.2 kbp that contains sequences highly similar to those of a plasmid detected in Vibrio shilonii, a coral pathogen. The detection of this plasmid was correlated with the highest pathogenicity status of the isolates from our collection. The origin and consequence of this plasmid acquisition are discussed. PMID:18359828

  10. Litopenaeus vannamei sterile-alpha and armadillo motif containing protein (LvSARM is involved in regulation of Penaeidins and antilipopolysaccharide factors.

    Directory of Open Access Journals (Sweden)

    Pei-Hui Wang

    Full Text Available The Toll-like receptor (TLR-mediated NF-κB pathway is tightly controlled because overactivation may result in severe damage to the host, such as in the case of chronic inflammatory diseases and cancer. In mammals, sterile-alpha and armadillo motif-containing protein (SARM plays an important role in negatively regulating this pathway. While Caenorhabditis elegans SARM is crucial for an efficient immune response against bacterial and fungal infections, it is still unknown whether Drosophila SARM participates in immune responses. Here, Litopenaeus vannamei SARM (LvSARM was cloned and functionally characterized. LvSARM shared signature domains with and exhibited significant similarities to mammalian SARM. Real-time quantitative PCR analysis indicated that the expression of LvSARM was responsive to Vibrio alginolyticus and white spot syndrome virus (WSSV infections in the hemocyte, gill, hepatopancreas and intestine. In Drosophila S2 cells, LvSARM was widely distributed in the cytoplasm and could significantly inhibit the promoters of the NF-κB pathway-controlled antimicrobial peptide genes (AMPs. Silencing of LvSARM using dsRNA-mediated RNA interference increased the expression levels of Penaeidins and antilipopolysaccharide factors, which are L.vannamei AMPs, and increased the mortality rate after V. alginolyticus infection. Taken together, our results reveal that LvSARM may be a novel component of the shrimp Toll pathway that negatively regulates shrimp AMPs, particularly Penaeidins and antilipopolysaccharide factors.

  11. Susceptibility to an inoculum of infectious hypodermal and haematopoietic necrosis virus (IHHNV in three batches of whiteleg shrimp Litopenaeus vannamei (Boone, 1931

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    Cesar Marcial Escobedo-Bonilla

    2014-11-01

    Full Text Available The present study evaluated the susceptibility of three different batches of whiteleg shrimp Litopenaeus vannamei from Mexico to an inoculum of infectious hypodermal and haematopoietic necrosis virus (IHHNV. Each of the three shrimp batches came from a different hatchery. Because of their origin, it was possible that the genetic makeup of these batches was different among each other. The three batches tested showed differences in IHHNV susceptibility. Here, susceptibility is defined as the capacity of the host to become infected, and it can be measured by the infectivity titer. Susceptibility to IHHNV was observed in decreasing order in shrimp from batch 1 (hatchery from El Rosario, Sinaloa, batch 3 (hatchery from Nayarit and batch 2 (hatchery from El Walamo, Sinaloa, respectively. The largest susceptibility difference between batches was 5012 times, and that between early and late juveniles from the same batch was 25 times. These results indicate that within a species, susceptibility to a pathogen such as IHHNV can have large differences. Susceptibility to pathogens is an important trait to consider before performing studies on pathogenesis. It may influence virological parameters such as speed of replication, pathogenicity and virus titer. In order to evaluate the potential use of IHHNV as a natural control agent against white spot syndrome virus (WSSV, it is necessary to know host susceptibility and the kinetics of IHHNV infection. These features can help to determine the conditions in which IHHNV could be used as antagonist in a WSSV infection.

  12. The protection of CpG ODNs and Yarrowia lipolytica harboring VP28 for shrimp Litopenaeus vannamei against White spot syndrome virus infection

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    Q Yi

    2014-04-01

    Full Text Available The white spot syndrome is one of the most serious disease which has caused high mortalities and huge economic losses to shrimp culture. In the present study, the oral administrations with CpG ODNs and Yarrowia lipolytica harboring VP28 (rVP28-yl as dietary supplement for shrimp Litopenaeus vannamei were conducted to evaluate their protective effects against WSSV. After feeding for 15 days, the cumulative mortality and the copy number of WSSV in CpG and rVP28-yl feeding shrimps were significantly lower when they were challenged by WSSV, compared with those in control shrimps (p < 0.05. The caspase-3 activity was suppressed in rVP28-yl feeding shrimps but ascended in CpG feeding shrimps after WSSV challenge. Besides, the PO activity in CpG feeding shrimps was significantly increased after feeding trial, and kept increasing post WSSV challenge (p < 0.05. While the increased NO production was observed both in CpG and rVP28-yl feeding shrimps after feeding trial and WSSV challenge. In addition, increased mRNA expression levels of STAT and Dicer were observed in CpG group post WSSV challenge. These results together indicated that oral feeding of CpG ODNs and rVP28-yl could enhance the innate non-specific immune responses especially antiviral immunity of shrimps in varying degrees, and increase their resistance against WSSV infection

  13. Genome survey and high-density genetic map construction provide genomic and genetic resources for the Pacific White Shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Yu, Yang; Zhang, Xiaojun; Yuan, Jianbo; Li, Fuhua; Chen, Xiaohan; Zhao, Yongzhen; Huang, Long; Zheng, Hongkun; Xiang, Jianhai

    2015-10-27

    The Pacific white shrimp Litopenaeus vannamei is the dominant crustacean species in global seafood mariculture. Understanding the genome and genetic architecture is useful for deciphering complex traits and accelerating the breeding program in shrimp. In this study, a genome survey was conducted and a high-density linkage map was constructed using a next-generation sequencing approach. The genome survey was used to identify preliminary genome characteristics and to generate a rough reference for linkage map construction. De novo SNP discovery resulted in 25,140 polymorphic markers. A total of 6,359 high-quality markers were selected for linkage map construction based on marker coverage among individuals and read depths. For the linkage map, a total of 6,146 markers spanning 4,271.43 cM were mapped to 44 sex-averaged linkage groups, with an average marker distance of 0.7 cM. An integration analysis linked 5,885 genome scaffolds and 1,504 BAC clones to the linkage map. Based on the high-density linkage map, several QTLs for body weight and body length were detected. This high-density genetic linkage map reveals basic genomic architecture and will be useful for comparative genomics research, genome assembly and genetic improvement of L. vannamei and other penaeid shrimp species.

  14. Effect of replacing fish meal with extruded soybean meal on growth, feed utilization and apparent nutrient digestibility of juvenile white shrimp ( Litopenaeus vannamei)

    Science.gov (United States)

    Yang, Qihui; Tan, Beiping; Dong, Xiaohui; Chi, Shuyan; Liu, Hongyu

    2015-10-01

    Extruded soybean meal (ESBM) was evaluated as a protein source for partial replacement of fish meal (FM) in diets of juvenile Litopenaeus vannamei. In the control diet (Diet 1), FM protein was replaced with increasing dietary levels of ESBM (4.28%, 8.40%, 12.62%, 16.82%, and 25.26%) at 10%, 20%, 30%, 40%, and 60% levels (Diets 2 to 6, respectively). An eight-week feeding trial was conducted on 720 juvenile shrimp (0.67 g ± 0.01 g mean initial weight), and nutrient digestibility of the six diets was determined. ESBM could replace 20% of FM without causing a significant reduction in growth of shrimp, but other dietary treatments strongly affected whole body composition. Crude protein content of the whole body fed Diet 6 was significantly lower than that fed Diet 2 ( P < 0.05), while crude lipid content of the whole body fed Diet 5 or 6 was significantly higher than that fed Diet 2 ( P < 0.05). Protein digestibilities of Diets 5 and 6 were significantly lower than that of Diet 1 ( P < 0.05). Digestibility of lipids ranged from 96.97% in Diet 6 to 98.34% in Diet 3, whereas dry matter digestibility decreased with increasing replacement level. This study indicates that 20% FM replacement with ESBM in the basic diet containing 40% protein and 30% FM is optimal for juvenile L. vannamei.

  15. Dietary supplementation of probiotic Bacillus PC465 isolated from the gut of Fenneropenaeus chinensis improves the health status and resistance of Litopenaeus vannamei against white spot syndrome virus.

    Science.gov (United States)

    Chai, Peng-Cheng; Song, Xiao-Ling; Chen, Guo-Fu; Xu, Hua; Huang, Jie

    2016-07-01

    This study conducted a 30-day feeding trial and a subsequent 20-day anti-virus infection trial to determine the effects of probiotic Bacillus PC465 on the growth, health status, and disease resistance of Litopenaeus vannamei. Shrimp samples were fed with three practical diets prepared from shrimp feed containing varying probiotic doses [0 (control), 10(7), and 10(9) CFU g(-1)]. Probiotic supplementation significantly increased the weight gain and survival of L. vannamei (p digestive enzymes, such as amylase, protease, and lipase, in the shrimp mid-gut significantly increased in the probiotic-fed groups on days 15 and 30, except lipase on day 30. The influence of 10(9) CFU g(-1) on enzyme activities was also greater than that of 10(7) CFU g(-1). Scanning electron microscopy revealed folds and large ravines across the interior surface of the mid-gut, and the number of these folds and ravines increased significantly after the probiotic was administered. The probiotic treatment significantly (p digestion, and nutrient absorption of L. vannamei. Probiotic treatment also enhances the microbial structures in the gut, promotes the immune status of shrimp, and provides protection against viral infection. The supplementation with 10(9) CFU g(-1) can also improve the growth and survival of L. vannamei. PMID:27177431

  16. Effect of the diet traditional and non-traditional on the respiration and excretion in larvae of white shrimp Litopenaeus vannamei

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    María Alejandra Medina-Jasso

    2015-11-01

    Full Text Available Objetive. It was studied the respiration and ammoniacal excretion of zoeas and mysis of Litopenaeus vannamei fed with the diet used traditionally (of microalgae and nauplios of artemia and another alternative (not traditional of microalgae with rotifers. Materials and methods. After four hours the oxygen consumption and ammonia excretion in BOD bottles with 60 larvae (closed respirometers was estimated. The concentrations of O2 and NH4 + were measured with an electrode polarográfico in the first case and with the indophenol blue technique for the second. Results. In zoea, oxygen consumption increased with development and showed statistical differences (p=0.023. In mysis, the oxygen consumption were significance in the traditional diet, whereas no differences were alternative (p=0.003. In both stages for the ammoniacal excretion increased development stage and there were detected statistical difference (p<0.001, although to the diets were not noticed significant differences. Conclusions. A higher energy absorption for zoea (I, II y III what mysis (I, II y III larvae was obtained, this is likely an interaction between rates of respiration and excretion caused by variations in the efficiency of absorption by the larvae. The weights obtained in both larvae were not supplied with differences between diets.

  17. Evaluation of the nutritional quality of Chaetoceros muelleri Schütt (Chaetocerotales: Chaetocerotaceae and Isochrysis sp. (Isochrysidales: isochrysidaceae grown outdoors for the larval development of Litopenaeus vannamei (Boone, 1931 (Decapoda: Penaeidae

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    Rodríguez Erika O.

    2012-01-01

    Full Text Available The biomass, proximal composition and fatty acid profile of Isochrysis sp., Chaetoceros muelleri and their mixture, grown under greenhouse conditions, were evaluated. The nutritional value of both species supplied as the monoalgal (Chaetoceros muelleri: Diet I, and Isochrysis sp. Diet II and mixed diet (Diet III for larval Litopenaeus vannamei was also assessed on the basis of the development and biochemical composition of the larvae. The highest protein levels were obtained in Diets I and II (40% and 35%, respectively. No significant differences in larval survival were found among the diets; however, larvae fed on Diet II had the lowest mean larval length.

  18. Ensayo para el cultivo del camarón marino litopenaeus vannamei Pérez-Farfante y Kensley, 1997, a baja salinidad en la estación del Mariel

    OpenAIRE

    Cabrera Machado, J.E.; Jaime Ceballos, B.; Galindo López, J.

    2010-01-01

    Con el objetivo de evaluar el cultivo del camarón marino Litopenaeus vannamei a baja salinidad, se ensayaron diferentes modificaciones del agua de pozo de una localidad cercana al río Mosquito (Mariel, La Habana, Cuba), previo análisis de su composición iónica. Se desarrolló un diseño completamente aleatorizado a escala de laboratorio, durante 14 días, utilizando postlarvas (PL20). Al agua se le realizaron las siguientes modificaciones: I) agua de pozo + sal bruta + sulfato de mag...

  19. An in vitro study of alkaline phosphatase sensitivity to mixture of aflatoxin B1 and fumonisin B1 in the hepatopancreas of coastal lagoon wild and farmed shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Pérez-Acosta, Jesús A; Burgos-Hernandez, Armando; Velázquez-Contreras, Carlos A; Márquez-Ríos, Enrique; Torres-Arreola, Wilfrido; Arvizu-Flores, Aldo A; Ezquerra-Brauer, J Marina

    2016-08-01

    This study aimed to establish the combined effect of aflatoxin B1 (AFB1) and fumonisin B1 (FB1) on wild Litopenaeus vannamei hepatopancreas alkaline phosphatase (AP) activity compared with that of farmed shrimp. AP activity in hepatopancreas extract was confirmed by several specific inhibitor assays. AP activity of wild shrimp was higher than that of farmed shrimp (p mycotoxins concentrations used in the study, AP from farmed L. vannamei was sensitive to the presence of both mycotoxins; however, AP is more sensitive to the combination of AFB1 + FB1 suggesting a possible synergistic or potentiating inhibitory effect. PMID:27040818

  20. Aclimatação do camarão marinho Litopenaeus vannamei (Boone, 1931 à água doce com diferentes estratégias de alimentação e calagem - DOI: 10.4025/actascianimsci.v28i1.668 Acclimatization of the marine shrimp Litopenaeus vannamei (Boone, 1931 to the freshwater with different feeding and liming strategies - DOI: 10.4025/actascianimsci.v28i1.668

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    Bruno Leonardo da Silva Santos

    2006-01-01

    Full Text Available Pós-Larvas do camarão Litopenaeus vannamei, na fase de PL9-10 e PL25-26 foram aclimatadas à água doce (0,0 ‰, objetivando promover cultivos em regiões interioranas. Para isso, foram realizados dois experimentos conduzidos em aquários com capacidade útil de 44 litros e densidade de estocagem de 11,36 pós-larvas/L. No experimento 1, foram testadas diferentes concentrações de cal hidratada (0,018 g a 0,031 g e 2 dietas, formuladas à base de ração comercial para camarão e biomassa de artêmia. No experimento 2, foram testadas 5 dietas com diferentes concentrações de biomassa de artêmia (0% a 100%, inseridas em rações de peixes e camarões. No final do processo de aclimatação, a sobrevivência das póslarvas variou de 40,44% a 98,04%. Concluiu-se que pós-larvas do Litopenaeus vannamei podem ser aclimatadas à água doce e que o aumento da concentração de cal e de artêmia na ração melhoraram significativamente (p Litopenaeus vannamei postlarvae with 9-10 (PL9-10, 25-26 days old (PL25-26 were acclimated to freshwater (0 ppt for the purpose of attending the inland cultures. Two experiments were conducted in 44 liter aquariums with stocking density of 11.36 postlarvae/L. In experiment 01, different concentrations of hydrated lime (0.018 g a 0.031 g and two diets, formulated with commercial shrimp ration and artemia biomass, were tested. In experiment 02, five diets, with different concentrations of artemia biomass (0 to 100% inserted in fish and shrimp rations, were tested. At the end of the acclimatization process, the postlarvae survival varied from 40.44 to 98.04%. Results showed that Litopenaeus vannamei postlarvae can be acclimated to freshwater. Also, the increase of lime concentration and the artemia biomass in the ration improved (p < 0.05 the zootechnic parameters of the species in a significant way.

  1. Bases para la elaboración del plan de ordenamiento territorial de la reserva de fauna silvestre Hueque-Sauca, municipio Píritu, estado Falcón

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    Miguel Antonio Flores

    2010-01-01

    Full Text Available En el presente trabajo se propone una zonificación para el ordenamiento territorial de la reserva de fauna silvestre Hueque-Sauca, ubicada en el Municipio Píritu del Estado Falcón. La misma se basó en el estudio de las potencialidades y limitaciones del medio físico-natural las cuales fueron expresadas en un atlas cartográfico de 22 mapas temáticos realizado en ARCGIS a escala 1: 25.000. Mediante la superposición y análisis de los mapas temáticos, se identificaron los usos y actividades realizados actualmente por los habitantes del RFS Hueque- Sauca y se contrastaron con los determinados por la potencialidad del área y por lo establecido en la legislación ambiental; a fin de elaborar una propuesta de zonificación que sirva de base para la elaboración de los instrumentos normativos para la gestión, conservación y manejo de esta área protegida.

  2. Contribución de Graells a la posición de España en el primer convenio internacional para la protección de ciertas especies de la fauna silvestre (1902

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    Ferrero-García, J. J.

    2012-12-01

    Full Text Available The process followed by Spain to decide whether it should subscribe the International Convention for the Protection of Birds Useful to Agriculture, signed in 1902 is described. The fundamental role played by Mariano de la Paz Graells, one of the foremost Spanish naturalists of the 19th century, in the technical review and acceptance of the draft agreement, drawn up in 1895, is highlighted. Furthermore, a critical analysis of Graells’s position in relation to this so-called first international convention for the conservation of certain wildlife species, has been carried out.

    Se describe el proceso que siguió España para decidir si debía suscribir el Convenio Internacional para la Protección de las Aves Útiles a la Agricultura, firmado en 1902. Se pone de manifiesto el papel fundamental que tuvo Mariano de la Paz Graells, uno de los más importantes naturalistas españoles del siglo XIX, en el examen técnico y aceptación del proyecto de dicho convenio, redactado en 1895. Además, se ha realizado un análisis crítico de la postura de Graells en relación con la que puede calificarse como la primera convención internacional para la conservación de ciertas especies de la fauna silvestre.

  3. DIAGNÓSTICO PARTICIPATIVO DE LAS CONDICIONES SOCIOCULTURALES ASOCIADAS A LA CONSERVACIÓN DE LOS CHILES SILVESTRES (Capsicum spp. EN LA DEPRESIÓN CENTRAL DE CHIAPAS, MÉXICO

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    R. A. A. Bran

    2007-01-01

    Full Text Available Los recursos fitogenéticos son la base de la seguridad alimentaria de la población mundial. México constituye uno de los centros de origen y diversidad de varias especies vegetales, entre ellas diversos tipos de Capsicum. Sin embargo, el mal manejo de ellos está afectando su conservación, fundamentalmente por la acción depredadora del hombre, el uso excesivo de agroquímicos y la deforestación; todo esto explica el esfuerzo de las instituciones científicas y gubernamentales por su cuidado y conservación. El trabajo se realizó en cinco municipios de la región Frailesca de la Depresión Central de Chiapas, México. Se inició con la aplicación de una encuesta a 386 pobladores de la región rural. La información fue evaluada mediante el análisis de frecuencia. Los resultados demostraron que la población rural tiene conocimientos sobre la utilización de los chiles silvestres y semisilvestres como alimento y condimento, no así en los aspectos relacionados con su conservación, la cual es realizada por los campesinos en pequeños huertos.

  4. Migraciones en el chaco semiárido de salta: su relación con la ganadería, la explotación forestal y el uso de la fauna silvestre en el departamento rivadavia

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    F. Barbar\\u00E1n

    2001-01-01

    Full Text Available Considerando que la Colonia Rivadavia se fundó en 1862, con el objetivo principal de aprovechar los pastizales del Chaco Semiárido para exportar ganado al Norte de Chile, analizamos desde una perspectiva histórica y demográfica, la relación existente entre las migraciones humanas y las principales actividades económicas del Departamento Rivadavia. Usando el método residual, se calcularon los saldos migratorios entre los censos nacionales de población y vivienda de 1914, 1947, 1960, 1970, 1980, 1991 y la población proyectada a 1998, resultando todos negativos. La expulsión constante de población, obedece al retroceso de la actividad ganadera por sobrepastoreo, lo que condujo a la degradación del ecosistema y la caída del ingreso, induciendo a los pobladores locales a presionar mas sobre el bosque y la fauna silvestre. La pobreza, consecuencia directa de un problema ambiental, obligó a emigrar de Rivadavia. Esta conclusión se sustenta en la evolución de distintos indicadores socio-económicos que se comparan para distintos períodos de los últimos treinta años: necesidades básicas insatisfechas, desnutrición infantil y el fracaso de distintos proyectos de desarrollo dirigidos principalmente a aborígenes en el período 1969 – 1992

  5. Presença de anticorpos da classe IgM de Leptospira interrogans em animais silvestres do Estado do Tocantins, 2002 Presence of IgM antibodies for Leptospira interrogans in wild animals from Tocantins State, 2002

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    Milton Formiga de Souza Júnior

    2006-06-01

    Full Text Available Quatrocentos e vinte e sete amostras de soro provenientes de animais silvestres foram testadas frente a 18 sorovariedades de Leptospira interrogans. De 286 amostras de Cebus apella, 46 (16,1% foram positivas para as sorovariedades pomona, brasiliensis, mini, swajizak, grippothyphosa, sarmin, fluminense, autumnalis, hebdomadis, guaratuba, javanica e icterohaemorhagiae. Das 82 de Alouatta caraya, 2 (2,4% foram positivas para as sorovariedades mangus e fluminense. Das 31 de Nasua nasua, 4 (12,9% foram positivas para as sorovariedades fluminense e javanica. Das 10 amostras de Cerdocyon thous, 2 (20% foram positivas para as sorovariedades fluminense e brasiliensis. Sete de Dasyprocta sp, 6 de Tamandua tetradactila e 5 de Euphractus sexcintus não apresentaram reatividade.Four hundred and twenty-seven serum samples of wild animals were tested against 18 serovars of Leptospira interrogans. Of 286 samples of Cebus apella, 46 (16.1% were positive for the serovars pomona, brasiliensis, mini, swajizak, grippotyphosa, sarmin, fluminense, autumnalis, hebdomadis, guaratuba, javanica and icterohaemorrhagiae. Of 82 samples of Alouatta caraya, 2 (2.4% were positive for the serovars mangus and fluminense. Of 31 samples of Nasua nasua, 4 (12.9% were positive for the serovars fluminense and javanica, and of 10 samples of Cerdocyon thous, 2 (20 % were positive for the serovars fluminense and brasiliensis. Seven samples of Dasyprocta sp, 6 of Tamandua tetradactyla and 5 of Euphractus sexcintus did not present reactivity.

  6. 凡纳对虾penaeidin2抗菌肽基因的克隆与性质研究%Gene Cloning and Characterization of Penaeidin 2 in Litopenaeus Vannamei

    Institute of Scientific and Technical Information of China (English)

    杜志强; 王建英

    2012-01-01

    Antimicrobial peptides directly play the immunity functions in the innate immunity system of the invertebrate. They play an important role in defending against the bacteria infection. In this paper, the Litopenaeus vannamei penaeidin 2 antibacterial peptides gene is the research object. And the gene cloning,amino acids sequences alignment, phylogenetic relationship analysis, and preliminary forecast for the molecular structure are mainly done. The results show that the Lva-pen 2 gene is an immunity response relevant gene. And it plays an important role in the innate immunity system of the Litopenaeus vannamei.%抗菌肽分子是无脊椎动物先天免疫系统中,直接发挥免疫效应的分子;它在对抗病原细菌侵染的过程中发挥着重要的功能.本文以凡纳对虾对虾素抗菌肽基因penaeidin2作为研究对象,系统地进行了基因克隆、氨基酸序列比对、分子进化关系以及分子结构的初步预测等研究.研究结果显示:Lva-pen2基因是一个免疫反应相关基因,它在凡纳对虾的先天免疫系统中发挥着重要功能.

  7. Phytoplanktonic composition of three cultivation systems used in Litopenaeus vannamei (BOONE, 1931 marine shrimp farms - doi: 10.4025/actascibiolsci.v32i3.4816

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    Ivone Ferreira Borges

    2010-09-01

    Full Text Available The aim of this work is to assess the different compositions of phytoplankton in three cultivation systems of marine shrimps Litopenaeus vannamei (BOONE, 1931, denominated as organic, intensive and semi intensive. The samples were done fortnightly, when phytoplankton was collected by a net for phytoplankton, 65 µm mesh, being then filtrated in a total volume of water of 100 L, and preserved in formaldehyde solution at 4% and identified according to the methodology of Cordeiro et al. (1997. The results show that the densities of Diatoms were of 16.65, 10.47 and 7.57 cel. 103 mL-1 for the organic, intensive and semi intensive cultivations, respectively. As for cyanobacteria, the average figures were 42.06 cel. 103 mL-1 for semi intensive 17.27 cel. 103 mL-1, in the intensive cultivation and 6.11 cel. 103 mL-1 for the organic cultivation system. The dinoflagellates had the highest cellular density in the phytoplankton community analyzed with 61.9 cel. 103 mL-1 in the intensive cultivation, 0.33 and 0.03 cel. 103 mL-1 for both semi intensive and organic cultivation systems respectively. Euglenas presented the results of 4.98 and 14.86 cel. 103 mL-1 only for semi intensive and intensive cultivations. It was then concluded that all cultivations presented average rates below recommended for such studied systems.The aim of this work is to assess the different compositions of phytoplankton in three cultivation systems of marine shrimps Litopenaeus vannamei (BOONE, 1931, denominated as organic, intensive and semi intensive. The samples were done fortnightly, when phytoplankton was collected by a net for phytoplankton, 65 µm mesh, being then filtrated in a total volume of water of 100 L, and preserved in formaldehyde solution at 4% and identified according to the methodology of Cordeiro et al. (1997. The results show that the densities of Diatoms were of 16.65, 10.47 and 7.57 cel. 103 mL-1 for the organic, intensive and semi intensive cultivations

  8. Sequence and Expression Analysis of COPE Gene from Litopenaeus vannmei%凡纳滨对虾COPE基因序列及低温表达分析

    Institute of Scientific and Technical Information of China (English)

    彭金霞; 蒋小珍; 房振峰; 殷勤; 韦嫔媛; 陈晓汉

    2013-01-01

    The cloning of cold tolerant COPE gene was studied in order to provide basis for the molecular mechanism study of Litopenaeus uan-namei cold tolerance characters. By using the homology cloning and RACE-PCR technology for Litopenaeus vannamei COPE gene (Lv-COPE) full-length cDNA sequencing, the bioinformatics analysis was conducted and the fluorescence quantitative PCR expression and Lo-COPE gene expression in tissues under low temperature stress was studied. Results showed that the LvCOPE cDNA length wasl217bp, which contained an 888 bp ORF which encoded a 296 Aa peptide. A conserved TPR region was found in the peptide, and the phylogenetic tree constructed by COPE protein reflected the evolutionary level of the species. The real-time PCR showed that the LvCOPE mRNA express in all tissues were mainly expressed in muscle. The LvCOPE mRNA was downwards regulated in the hepatocirrhosis, heart, gill, and muscle areas of shrimp treated under low temperature. Therefore, LvCOPE was conserved in gene structure and evolution relationship, and the downwards regulation of mRNA under low temperature may related to its cold tolerance function.%通过对已知耐寒候选基因-COPE基因的克隆和研究,为凡纳滨对虾耐寒性状的分子机理研究提供依据.运用同源克隆和RACE-PCR技术获得凡纳滨对虾COPE基因(LvCOPE)全长cDNA序列,对其进行了生物信息学分析,采用荧光定量PCR研究了LvCOPE基因的组织表达谱及其在低温胁迫下表达量的变化.结果显示,LvCOPEcDNA全长1217 bp,包含888 bp开放阅读框,编码296个氨基酸残基,具有保守的TPR结构域.各物种COPE蛋白序列构建的系统进化树能准确反映各物种间的进化关系.IvCOPE mRNA在各组织中呈遍在表达,在肌肉组织中表达量最高.低温表达谱分析显示,bCOPE mRNA在低温处理对虾的肝胰腺、心、鳃、肌肉等组织中均呈下调表达,随着处理温度由15℃降至11℃度,其在肝胰腺中表达量逐渐降

  9. Seleção de variáveis em modelos matemáticos dos parâmetros de cultivo do camarão marinho Litopenaeus vannamei Selection of variables in mathematical models of culture parameters of marine shrimp Litopenaeus vannamei

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    Ady Marinho Bezerra

    2007-03-01

    Full Text Available O objetivo deste trabalho foi selecionar as variáveis de manejo do camarão marinho Litopenaeus vannamei que mais influenciaram nas variáveis-respostas ao cultivo (produção, produtividade, peso final e taxa de sobrevivência, em modelos matemáticos. O banco de dados foi composto por 83 cultivos, realizados no período de 2003 a 2005, obtidos de uma fazenda comercial localizada no litoral sul de Pernambuco. Para estimar os parâmetros dos modelos, utilizou-se a técnica dos mínimos quadrados. A seleção das variáveis foi realizada com o processo "backward elimination" associado ao método de transformação de Box e Cox. A adequação das equações e os pressupostos de normalidade e homocedasticidade, para os erros, foram analisadas com base na análise de variância e análise de resíduo. É possível relacionar essas variáveis e estabelecer predições com as equações.The objective of this work was to select management variables of the marine shrimp Litopenaeus vannamei that most influenced culture variable responses (production, productivity, final weight and survival rate, in mathematical models. The database was composed of 83 cultures in the period of 2003 to 2005, obtained from a shrimp farm located in the South coast of Pernambuco. To estimate the parameters of the models it was used the technique of least square. The selection of variable was carried through the backward elimination process associated to the Box and Cox transformation. The adequacy of the equations and the hypothesis of normality and homogeneous variance for the errors were analyzed based on the analysis of variance and on the analysis of residuals. It is possible to correlate those variables and to establish predictions with the equations.

  10. Influência do aquecimento sobre diferentes métodos de titulação de SO2 residual em camarões Litopenaeus vannamei (Boone, 1931 = Influence of heating under different titration methods of residual SO2 on shrimps Litopenaeus vannamei (Boone, 1931

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    Karla Patrícia Brito de Araújo Vieira

    2008-01-01

    Full Text Available O metabissulfito de sódio é o conservante mais aplicado na carcinicultura, com uso amparado em legislações vigentes no país, todavia, seu resíduo, dióxido de enxofre (SO2, em concentrações altas, pode provocar reações adversas à saúde. Este estudo foidesenvolvido com a finalidade de observar a influência do aquecimento sob diferentes métodos de titulação de SO2 residual em camarões Litopenaeus vannamei. Os camarões foram submetidos a nove concentrações de metabissulfito de sódio e avaliados com diferentesmétodos de detecção de SO2. O método da titulação Adolfo Lutz adaptado apresentou grande sensibilidade para detecção de SO2 residual. O método da titulação iodométrica com aquecimento possibilitou a detecção de maiores níveis de SO2, quando comparado aométodo sem o aquecimento. O aquecimento interferiu negativamente no método da fita reativa. Conclui-se que o aquecimento influenciou positivamente a titulação iodométrica, não ocorrendo o mesmo com a fita reativa.Sodium metabisulfite is the most applied preservative in shrimp culture, with its use supported by current legislation; however, its residue, sulfur dioxide (SO2, in high concentrations can provoke adverse health reactions. This study evaluated the influence of heating under different methods of residual SO2 titration in Litopenaeus vannamei. Shrimps were treated with nine concentrations of sodium metabisulfite and evaluated by different methods of SO2 titration. The adapted Adolfo Lutz titration method presented the highest sensitivity for the detection of SO2. The method of iodometric titration with heating made possible the detention ofhigher levels of SO2, when compared to the same method without heating. The heating influenced negatively on the reactive ribbon method. It is concluded that the heating influenced positively on the iodometric titration, as opposed to the reactive ribbon.

  11. Efeito da adição do melaço na relação carbono/nitrogênio no cultivo de camarão Litopenaeus vannamei na fase berçário = Effect of molasses addition on carbon/nitrogen ratio in the nursery phase of Litopenaeus vannamei shrimp culture

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    Ugo Lima Silva

    2009-10-01

    Full Text Available Investigou-se o efeito da adição do melaço nas relações carbono:nitrogênio (C:N sobre o desempenho zootécnico do camarão Litopenaeus vannamei, na fase berçário, quando cultivado sem renovação de água. As relações do C:N foram avaliadas nas proporções de 25:1 (25M, 15:1 (15M e o controle (0M, sem aplicação de carbono, em delineamento experimental inteiramente casualizado, com quatro repetições. Pós-larvas (PL com peso inicial de 2,5 ± 0,5 mg, foram estocadas em 12 tanques (800 L volume útil, em densidades de 6,25 PL L-1, durante 42 dias de cultivo. Ao final do cultivo, os pesos finais dos camarões dos tratamentos 25M (532,0 mg e 15M (540,0 mg foram superiores (p The effect of molasses addiction on carbon:nitrogen ratios (C:N on the performance of Litopenaeus vannamei shrimp during the nursery phase cultured without water exchange was investigated. The C:N ratios were evaluated in 25:1 (25M and 15:1 (15M proportion and a control (0M, with no carbon source addition, in a randomized experimental design with four replicates. Post-larvae (PL with initial weight of 2.5 ± 0.5 mg were stocked in 12 tanks (800 L net volume, at the density of 6.25 PL L-1 during 42 culture days. At the end of culture, the shrimps weights on 25M (532.0 mg and 15M (540.0 mg treatments were higher (p < 0.05 than the 0M (428.6 mg treatment. Shrimp survival was high in all treatments (77.9 to 90.0%, but without significant difference (p ≥ 0.05. Therefore, the 15 and 25:1 C:N ratios showed an increased growth performance of nursery phase L. vannamei post-larvae cultured without water exchange.

  12. Primer registro de la utilización de harinas de Salicornia bigelovii y Scomber japonicus en dietas prácticas para el cultivo súper-intensivo de camarón Litopenaeus stylirostris First record on the use of Salicornia bigelovii and Scomber japonicus fishmeals as feed for Litopenaeus stylirostris under super-intensive farming

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    Manuel de J . Acosta-Ruiz

    2011-11-01

    Full Text Available Se evaluó el efecto de las harinas de Salicornia bigelovii (SA y Scomber japonicus, semiprocesada (HPS como ingredientes en la formulación de dietas para camarón azul Litopenaeus stylirostris, en cultivo súper-intensivo. Se formularon tres diferentes dietas isoproteicas (40% e isocalóricas (6 kcal g-1: (DSA, (DHPS, basal (DBA y una dieta control (DCO. El peso obtenido con DSA y DHPS (0,9 ± 0,014 y 0,8 ± 0,015 g fue similar a la dieta comercial DCO (0,9 ± 0,07 g, no existieron diferencias significativas (P > 0,05 en talla (cm, peso (g y factor de conversión alimenticio (FCA. Los resultados obtenidos sugieren que DSA y DHPS son factibles de utilizar en la formulación de dietas para camarón por ser ingredientes de bajo costo que pueden sustituir a la harina de maíz y pescado tradicional, respectivamente sin efectos detrimentales en el crecimiento y supervivencia.The effect of semi-processed fishmeals (HPS made using Salicornia bigelovii (SA and Scomber japonicusas ingredients in the formulation of diets for blue shrimp Litopenaeus stylirostris undersuper-intensive farming was evaluated. Three different isoproteinic (40% and isocaloric (6 kcal g-1 diets were formulated: DSA, DHPS, basal (DBA; a fourth control diet (DCO was also used. The weights obtained with DSA and DHPS (0.9 ± 0.014 and 0.8 ± 0.015 g were similar to those obtained with the commercial diet (DCO; 0.9 ± 0.07 g, and no significant differences (P > 0.05 were found for size (cm, weight (g, and the feed conversion ratio (FCR. The results suggest that the use of DSA and DHPS in the formulation of diets for shrimp is feasibleas these are low-cost ingredients that can be used as substitutes for cornstarch and traditional fishmeal, respectively, without detrimental effects for shrimp growth and survival.

  13. Evaluación de la actividad enzimática y contenido de proteína en larvas de camarón blanco Litopenaeus vannamei alimentadas con diferentes dietas (Evaluation of the enzymatic activity and protein content in larvae of white shrimp Litopenaeus vannamei fed with different diets

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    Isiordia-Pérez E

    2007-08-01

    Full Text Available El alimento vivo para el cultivo de camarón requiere especial atención. Los alimentos comúnmente empleados en esta industria, se han seleccionado principalmente por la facilidad de su cultivo más que por sus propiedades nutricionales. El presente trabajo compara el uso de copépodos (Tisbe monozota vivos y muertos y una dieta microligada y microparticulada como alternativa alimenticia de nauplios de Artemia para larvas de camarón evaluando la actividad enzimática y contenido de proteínas de las misma. El estudio se realizó en estadios larvales de camarón blanco Litopenaeus vannamei bajo condiciones controladas de temperatura (28°C, salinidad (35‰ y fotoperiodo (12 h luz / 12h oscuridad. En ambos experimentos el mayor contenido proteico se obtuvo en larvas alimentadas con la dieta experimental y con Artemia viva. a diferencia de la actividad enzimática que fue mayor al utilizar copépodos tanto vivos como muertos. Los resultados demostraron que no hay diferencia significativa en contenido proteico y en actividad de tripsina al usar copépodos (vivos o muertos y nauplios de Artemia , y sugieren a la dieta experimental como buena fuente alternativa en los primeros estadios larvales de camarón blanco L. vannamei The live food for the shrimp culture requires special attention. The food commonly used in this industry, have been selected mainly for the feasibility of their culture rather than their nutritional properties. This present work include alive and dead copepods (Tisbe monozota and a microparticulate microbound diet as alternative live food to Artemia nauplii for shrimp larvae, evaluating their enzymatic activity and protein content. The study was made with larval stages of white shrimp Litopenaeus vannamei under control culture conditions: temperature (28°C, salinity (35% and photoperiodo (12 h light/12h dark. In both experiments the higher protein content was obtained in larvae fed with the microdiet and Artemia nauplii

  14. Comportamento de Litopenaeus vannamei (Boone (Crustacea, Decapoda, Penaeidae em função da oferta do alimento artificial nas fases clara e escura do período de 24 horas Behavior of Litopenaeus vannamei (Boone (Crustacea, Decapoda, Penaeidae in relation to artificial food offer along light and dark phases in a 24 h period

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    Cibele S. Pontes

    2005-09-01

    Full Text Available A escassez de dados acerca do comportamento do camarão marinho Litopenaeus vannamei (Boone, 1931 relacionado ao alimento artificial ofertado em comedouros poderá induzir a uma alimentação inadequada, aumentando a relação custo/benefício e os impactos ambientais potenciais do seu cultivo. Objetivando fornecer subsídios para a melhoria do manejo alimentar praticado nas fazendas, foram desenvolvidos estudos comportamentais utilizando 64 juvenis de L. vannamei (7,57 ± 1,01 g, submetidos a fotoperíodo artificial, metade deles em ciclo invertido, para observação das suas atividades durante as fases clara e escura. A ração foi oferecida em intervalos pré-estabelecidos, registrando-se antes e depois da oferta: exploração, natação, alimentação e inatividade (focal instantâneo. A natação ocorreu preferencialmente durante a fase escura. A alimentação foi mais elevada na primeira meia hora posterior à oferta, com ênfase nos horários da fase clara. O alimento artificial atuou, em ambas as fases, como um indutor da exploração do substrato, tornando os camarões mais ativos em todos os horários posteriores à sua oferta.The few data on the marine shrimp Litopenaeus vannamei (Boone, 1931 behavior related to artificial food offer in feeding trays may result an inadequate feeding by the animal, increasing the cost/benefit relation in shrimp culture and its potential environmental impact. In order to provide tools for optimizing feeding management in shrimp farms, a behavioral study was developed, using 64 L. vannamei juveniles (7,57 ± 1,01 g. They were submitted to artificial photoperiods, half of them in reversed cycle, in order to register their behavior during light and dark phases. In established intervals, the following activities were registered before and after food exposition: exploration, swimming, feeding and inactivity (instantaneous sampling. Swimming occurred mostly during the dark phase. Feeding time was higher in

  15. A preliminary evaluation of the San Pedro daisy (Lasianthaea podocephala tuber powder, as a feed additive on the intensive culture of shrimp (Litopenaeus vannamei under laboratory conditions

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    Marcel Martínez-Porchas

    2013-07-01

    Full Text Available An experimental study was carried out during seven weeks to preliminarily evaluate the effect of the dietary inclusion of powder from the tuber Lasianthaeapodocephala, commonly known as the San Pedro daisy or pionilla, as a feed additive on the production parameters, food consumption, and nutritional condition of the white shrimp, Litopenaeus vannamei, intensively farmed under laboratory conditions. Hypothetically, the additive should improve these parameters as it does when used for humans. The powder was included at different levels (0 [Control], 0.2 [T1] and 1 g kg-1 [T2] in a commercial shrimp feed. The tuber powder exhibited a free amino acid profile being arginine (8.59 mg g-1 and glutamine (3.36 mg g-1 the most abundant. Feed consumption was not influenced by any treatment; however, the survival and the overall production responses were higher in both treatments using the powder (T1 and T2 compared to the control. No significant differences were detected in muscle concentrations of lactate and glucose, although higher protein and lower cholesterol concentrations were observed in shrimp reared in the control. The lower cholesterol concentration of shrimp from the control could be associated to a poor nutritional status. In conclusion, addition of the powder of San Pedro daisy did not improve the feed consumption, but apparently had a positive effect on survival, production response and nutritional status of shrimp. These responses could be associated to an hypothetic effect of some plant components at a nutritional-molecular level, or to a possible antimicrobial effect; however, further specific studies are needed.

  16. Metabolic enzyme activities, metabolism-related genes expression and bioaccumulation in juvenile white shrimp Litopenaeus vannamei exposed to benzo[a]pyrene.

    Science.gov (United States)

    Ren, Xianyun; Pan, Luqing; Wang, Lin

    2014-06-01

    The purpose of this study was to investigate the impact of benzo[a]pyrene (BaP) on metabolic detoxification system and bioaccumulation of white shrimp Litopenaeus vannamei. In this study, juvenile white shrimp L. vannamei were exposed for 21 days at four different concentrations of 0, 0.03, 0.3 and 3μg/L. Detoxification enzyme activities of phase I (aryl hydrocarbon hydroxylase (AHH), 7-ethoxyresorufin O-deethylase (EROD), epoxide hydrolase (EH)) and phase II (glutathione-S-transferase (GST), sulfotransferase (SULT), uridine diphosphate glucuronyl transferase (UGT)) were determined, and results showed that all the detoxification enzyme activities increased in a dose-dependent manner except for the low BaP exposure. Transcription of genes was detected and measured by real-time RT-PCR. It showed that at day six BaP increased cytochrome P450 (CYP) 1A1, GST, SULT visa aryl hydrocarbon receptor (AhR) mRNA expression in a dose-dependent manner, which suggests that they could be potential targets of BaP that disrupt the detoxification system. The consistency of their responses to BaP exposure implies that AhR action may be involved in invertebrate CYP regulation. Additionally, BaP bioaccumulation increased rapidly first and showed an incoming plateau. Besides, the enzyme activities and bioaccumulation in the hepatopancreas were higher than those in the gills. These results will not only provide information on BaP metabolic mechanism for this species, but also scientific data for pollution monitoring. PMID:24636950

  17. Effects of biofloc promotion on water quality, growth, biomass yield and heterotrophic community in Litopenaeus vannamei (Boone, 1931 experimental intensive cultures

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    Irasema E. Luis-Villaseñor

    2015-08-01

    Full Text Available Six 1.2-m3 tanks were stocked with an initial biomass of 500 g m-3 of Litopenaeus vannamei juveniles (individual weight: 1.0±0.3 g, to evaluate the effect of biofloc promotion on water quality and on shrimp growth and production, and to identify the dominant taxa in the heterotrophic communities present in experimental closed cultures. Feeding was ad libitum twice daily with 35% protein shrimp feed. Three tanks were managed as biofloc technology (BFT systems, adding daily an amount of cornmeal equivalent to 50% of the shrimp feed supplied. The remaining three received only shrimp feed and served as controls. Experiment lasted 21 days. The mean concentrations of P-PO4 3- and inorganic dissolved N species (TAN, N-NO2 -, N-NO3 - were significantly lower (P<0.5 in BFT than in the control. The individual final weight, increase in biomass, food, and protein conversion rates were significantly better in BFT than in the control (P<0.05. The mean N content of the shrimp biomass gained in the BFT cultures was equivalent to 45.7% of the protein-N added as feed, and was significantly higher than the 34.7% recycled into shrimp biomass in the control cultures. Bacterial concentrations were not significantly different. Vibrionaceae dominated in both systems; in both some isolates were potential pathogens, and diversity was higher in the control than in the BFT treatment. The advantages of BFT technology are confirmed by the significantly lower TAN and N-NO2 - concentrations, as well as by the better shrimp performance in terms of growth, biomass yield, and food and protein conversion efficiency.

  18. Validation of a commercial insulated isothermal PCR-based POCKIT test for rapid and easy detection of white spot syndrome virus infection in Litopenaeus vannamei.

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    Yun-Long Tsai

    Full Text Available Timely pond-side detection of white spot syndrome virus (WSSV plays a critical role in the implementation of bio-security measures to help minimize economic losses caused by white spot syndrome disease, an important threat to shrimp aquaculture industry worldwide. A portable device, namely POCKIT™, became available recently to complete fluorescent probe-based insulated isothermal PCR (iiPCR, and automatic data detection and interpretation within one hour. Taking advantage of this platform, the IQ Plus™ WSSV Kit with POCKIT system was established to allow simple and easy WSSV detection for on-site users. The assay was first evaluated for its analytical sensitivity and specificity performance. The 95% limit of detection (LOD of the assay was 17 copies of WSSV genomic DNA per reaction (95% confidence interval [CI], 13 to 24 copies per reaction. The established assay has detection sensitivity similar to that of OIE-registered IQ2000™ WSSV Detection and Protection System with serial dilutions of WSSV-positive Litopenaeus vannamei DNA. No cross-reaction signals were generated from infectious hypodermal and haematopoietic necrosis virus (IHHNV, monodon baculovirus (MBV, and hepatopancreatic parvovirus (HPV positive samples. Accuracy analysis using 700 L. vannamei of known WSSV infection status shows that the established assayhassensitivity93.5% (95% CI: 90.61-95.56% and specificity 97% (95% CI: 94.31-98.50%. Furthermore, no discrepancy was found between the two assays when 100 random L. vannamei samples were tested in parallel. Finally, excellent correlation was observed among test results of three batches of reagents with 64 samples analyzed in three different laboratories. Working in a portable device, IQ Plus™ WSSV Kit with POCKIT system allows reliable, sensitive and specific on-site detection of WSSV in L. vannamei.

  19. Immunity enhancement with administration of Gracilaria corticata and Saccharomyces cerevisiae compared to gamma irradiation in expose to WSSV in shrimp, in juvenile Litopenaeus vannamei: A comparative study.

    Science.gov (United States)

    Afsharnasab, Mohammad; Kakoolaki, Shapour; Mohammadidost, Mehrdad

    2016-09-01

    This paper investigates the efficacy of Gracilaria corticata, Saccharomyces cerevisiae and gamma irradiation WSSV as immunostimulants to white shrimp Litopenaeus vannamei. Seven hundred and twenty healthy shrimp SPF L. vannamei subadult with average weight of 10 ± 1.02 g were collected and divided into 8 groups. The first group (T1) was fed with commercial pellet, the second group (T2) fed with S. cerevisiae (2 g/kg), the third group (T3) fed with G. corticata powder mixed with shrimp feed (2 g/kg) and, finally, the fourth group (T4) was fed with commercial pellet and injected intramuscularly gamma irradiant WSSV (1 μl/gbw) for 10 days. The shrimps were then injected with WSSV and maintained for 25 days. The positive control group for each treatment was maintained in the same manner but without injection with WSSV. Moreover, survival rate and immune parameters such as total hemocyte count (THC), total protein plasma (TPP), superoxide dismutase (SOD) activity, peroxidase (POD) activity and phenoloxidase activity (PO) were determined. Results indicated that the survival rates for groups T4, T3 T2 and T1 were 57.05 ± 3.52%, 22.5 ± 0.5%, 15 ± 1.05% and 00.0 ± 0%, respectively. Ultimately, at the end of the study the shrimp group T4 showed higher hematological data: THC, TPP, SOD, POD and PO. The study concluded that gamma irradiant WSSV is effective immunostimulants in shrimp L. vannamei and the immunity has better performances than those of the G. corticata and S. cerevisiae.

  20. An immersion of Gracilaria tenuistipitata extract improves the immunity and survival of white shrimp Litopenaeus vannamei challenged with white spot syndrome virus.

    Science.gov (United States)

    Lin, Yong-Chin; Yeh, Su-Tuen; Li, Chang-Che; Chen, Li-Li; Cheng, Ann-Chang; Chen, Jiann-Chu

    2011-12-01

    The innate immunity and resistance against white spot syndrome virus (WSSV) in white shrimp Litopenaeus vannamei which received the Gracilaria tenuistipitata extract were examined. Shrimp immersed in seawater containing the extract at 0 (control), 400 and 600 mg L(-1) for 3 h were challenged with WSSV at 2 × 10(4) copies shrimp(-1). Shrimp not exposed to the extract and not received WSSV challenge served as unchallenged control. The survival rate of shrimp immersed in 400 mg L(-1) or 600 mg L(-1) extract was significantly higher than that of challenged control shrimp over 24-120 h. The haemocyte count, phenoloxidase activity, respiratory burst, superoxide dismutase activity, and lysozyme activity of shrimp immersed in 600 mg L(-1) extract were significantly higher than those of unchallenged control shrimp at 6, 6, 6, 6, and 6-24 h post-challenge. In another experiment, shrimp which had received 3 h immersion of 0, 400, 600 mg L(-1) extract were challenged with WSSV. The shrimp were then received a booster (3 h immersion in the same dose of the extract), and the immune parameters were examined at 12-120 h post-challenge. The immune parameters of shrimp immersed in 600 mg L(-1) extract, and then received a booster at 9, 21, and 45 h were significantly higher than those of unchallenged control shrimp at 12-48 h post-challenge. In conclusion, shrimp which had received the extract exhibited protection against WSSV as evidenced by the higher survival rate and higher values of immune parameters. Shrimp which had received the extract and infected by WSSV showed improved immunity when they received a booster at 9, 21, and 45 h post-WSSV challenge. The extract treatment caused less decrease in PO activity, and showed better performance of lysozyme activity and antioxidant response in WSSV-infected shrimp.

  1. Dietary administration of a Gracilaria tenuistipitata extract enhances the immune response and resistance against Vibrio alginolyticus and white spot syndrome virus in the white shrimp Litopenaeus vannamei.

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    Sirirustananun, Nuttarin; Chen, Jiann-Chu; Lin, Yong-Chin; Yeh, Su-Tuen; Liou, Chyng-Hwa; Chen, Li-Li; Sim, Su Sing; Chiew, Siau Li

    2011-12-01

    The haemogram, phenoloxidase (PO) activity, respiratory bursts (RBs), superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity, lysozyme activity, and the mitotic index of haematopoietic tissue (HPT) were examined after the white shrimp Litopenaeus vannamei had been fed diets containing the hot-water extract of Gracilaria tenuistipitata at 0 (control), 0.5, 1.0, and 2.0 g kg(-1) for 7-35 days. Results indicated that these parameters directly increased with the amount of extract and time, but slightly decreased after 35 days. RBs, SOD activity, and GPx activity reached the highest levels after 14 days, whereas PO and lysozyme activities reached the highest levels after 28 days. In a separate experiment, white shrimp L. vannamei, which had been fed diets containing the extract for 14 days, were challenged with Vibrio alginolyticus at 2 × 10(6) cfu shrimp(-1) and white spot syndrome virus (WSSV) at 1 × 10(3) copies shrimp(-1), and then placed in seawater. The survival rate of shrimp fed the extract-containing diets was significantly higher than that of shrimp fed the control diet at 72-144 h post-challenge. We concluded that dietary administration of the G. tenuistipitata extract at ≤1.0 g kg(-1) could enhance the innate immunity within 14 days as evidenced by the increases in immune parameters and mitotic index of HPT in shrimp and their enhanced resistance against V. alginolyticus and WSSV infections. Shrimp fed the extract-containing diets showed a higher and continuous increase in the humoral response indicating its persistent role in innate immunity.

  2. Transcriptome and Molecular Pathway Analysis of the Hepatopancreas in the Pacific White Shrimp Litopenaeus vannamei under Chronic Low-Salinity Stress.

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    Ke Chen

    Full Text Available The Pacific white shrimp Litopenaeus vannamei is a euryhaline penaeid species that shows ontogenetic adaptations to salinity, with its larvae inhabiting oceanic environments and postlarvae and juveniles inhabiting estuaries and lagoons. Ontogenetic adaptations to salinity manifest in L. vannamei through strong hyper-osmoregulatory and hypo-osmoregulatory patterns and an ability to tolerate extremely low salinity levels. To understand this adaptive mechanism to salinity stress, RNA-seq was used to compare the transcriptomic response of L. vannamei to changes in salinity from 30 (control to 3 practical salinity units (psu for 8 weeks. In total, 26,034 genes were obtained from the hepatopancreas tissue of L. vannamei using the Illumina HiSeq 2000 system, and 855 genes showed significant changes in expression under salinity stress. Eighteen top Kyoto Encyclopedia of Genes and Genomes (KEGG pathways were significantly involved in physiological responses, particularly in lipid metabolism, including fatty-acid biosynthesis, arachidonic acid metabolism and glycosphingolipid and glycosaminoglycan metabolism. Lipids or fatty acids can reduce osmotic stress in L. vannamei by providing additional energy or changing the membrane structure to allow osmoregulation in relevant organs, such as the gills. Steroid hormone biosynthesis and the phosphonate and phosphinate metabolism pathways were also involved in the adaptation of L. vannamei to low salinity, and the differential expression patterns of 20 randomly selected genes were validated by quantitative real-time PCR (qPCR. This study is the first report on the long-term adaptive transcriptomic response of L. vannamei to low salinity, and the results will further our understanding of the mechanisms underlying osmoregulation in euryhaline crustaceans.

  3. Efecto de la inulina y del ácido fúlvico en la supervivencia, crecimiento, sistema inmune y prevalencia de WSSV en Litopenaeus vannamei

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    Anayeli Gutiérrez-Dagnino

    2015-11-01

    Full Text Available Se estudió el efecto del prebiótico inulina y ácido fúlvico, adicionados en el alimento, sobre el crecimiento, supervivencia, prevalencia de WSSV y sistema inmune de Litopenaeus vannamei. Para esto, se realizó un bioensayo, con tratamientos por triplicado, donde se probaron diferentes concentraciones de los aditivos. Se hizo un análisis de WSSV en organismos infectados con una carga viral relativamente alta utilizando la PCR sencilla y anidada. Al final del bioensayo se extrajo la hemolinfa y se estudió el sistema inmune en hemocitos a nivel bioquímico y genético (PCR cuantitativo. El peso final fue similar en todos los tratamientos y la supervivencia estuvo entre 66,7% y 93,3%. La prevalencia de WSSV disminuyó un 13% respecto al control. El número de hemocitos, la actividad de la fenoloxidasa y la concentración de anión superóxido fueron similares en todos los tratamientos. Los aditivos modularon la expresión de los genes transglutaminasa, superóxido dismutasa y profenoloxidasa, pero no la del receptor Toll. Los aditivos no afectan negativamente el crecimiento y protegen al camarón contra WSSV en organismos infectados con una carga viral relativamente alta. No se observó efecto de los aditivos en los efectores del sistema inmune estudiados a nivel bioquímico pero si modularon la expresión de algunos genes relacionados con el sistema inmune en L. vannamei.

  4. Rapid multiplex polymerase chain reaction for simultaneous detection of Vibrio harveyi, V. parahaemolyticus, and V. vulnificus in pacific white shrimp (Litopenaeus vannamei

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    Kanittada Thongkao

    2016-01-01

    Full Text Available Context: A comparatively small number of species, e.g., Vibrio parahaemolyticus and V. vulnificus, cause disease in both aquatic animals and humans. V. harveyi is marine animal pathogen and rarely causes infections in humans; however, it might become a reservoir of antibiotic-resistant bacteria forms and virulence genes. Aims: 1 to develop rapid multiplex polymerase chain reaction (PCR assay for the simultaneous detection of V. harveyi, V. parahaemolyticus, and V. vulnificus by using vhhP2, tl, and rpoS genes as the respective target genes and 2 to evaluate specificity and determined detection of multiplex PCR technique. Materials and Methods: The multiplex PCR assay was developed and evaluated for specificity on 36 isolates of V. harveyi, 30 isolates of V. parahaemolyticus, and 14 isolates of V. vulnificus, along with other species of Vibrio and non-Vibrio bacterial isolates. Sensitivity of test was described as detection limit of pathogens in lowest amount of sample (CFU/mL or CFU/g was determined by diluted DNA extracts of the pure cultures and spiked pacific white shrimp (Litopenaeus vannamei samples Results: This developed multiplex PCR was proved as an accurate method, which was specific for three Vibrio species. The detection limits of V. harveyi, V. parahaemolyticus, and V. vulnificus in pure cultures and spiked shrimp samples ranged 1.05-4.8 × 103 CFU/mL and 1.9-7 × 104 CFU/g, respectively. Conclusions: This rapid multiplex PCR assay can decrease amount and process of sample preparation, which was time-consuming, and had preferable accuracy. This developed technique will be suitable and useful for food-borne pathogen detection in shrimp and horizontal gene transfer study among different Vibrio species in aquatic animals.

  5. Immunity enhancement with administration of Gracilaria corticata and Saccharomyces cerevisiae compared to gamma irradiation in expose to WSSV in shrimp, in juvenile Litopenaeus vannamei: A comparative study.

    Science.gov (United States)

    Afsharnasab, Mohammad; Kakoolaki, Shapour; Mohammadidost, Mehrdad

    2016-09-01

    This paper investigates the efficacy of Gracilaria corticata, Saccharomyces cerevisiae and gamma irradiation WSSV as immunostimulants to white shrimp Litopenaeus vannamei. Seven hundred and twenty healthy shrimp SPF L. vannamei subadult with average weight of 10 ± 1.02 g were collected and divided into 8 groups. The first group (T1) was fed with commercial pellet, the second group (T2) fed with S. cerevisiae (2 g/kg), the third group (T3) fed with G. corticata powder mixed with shrimp feed (2 g/kg) and, finally, the fourth group (T4) was fed with commercial pellet and injected intramuscularly gamma irradiant WSSV (1 μl/gbw) for 10 days. The shrimps were then injected with WSSV and maintained for 25 days. The positive control group for each treatment was maintained in the same manner but without injection with WSSV. Moreover, survival rate and immune parameters such as total hemocyte count (THC), total protein plasma (TPP), superoxide dismutase (SOD) activity, peroxidase (POD) activity and phenoloxidase activity (PO) were determined. Results indicated that the survival rates for groups T4, T3 T2 and T1 were 57.05 ± 3.52%, 22.5 ± 0.5%, 15 ± 1.05% and 00.0 ± 0%, respectively. Ultimately, at the end of the study the shrimp group T4 showed higher hematological data: THC, TPP, SOD, POD and PO. The study concluded that gamma irradiant WSSV is effective immunostimulants in shrimp L. vannamei and the immunity has better performances than those of the G. corticata and S. cerevisiae. PMID:27377028

  6. Effect of various Na/K ratios in low-salinity well water on growth performance and physiological response of Pacific white shrimp Litopenaeus vannamei

    Science.gov (United States)

    Liu, Hongyu; Tan, Beiping; Yang, Jinfang; Lin, Yingbo; Chi, Shuyan; Dong, Xiaohui; Yang, Qihui

    2014-09-01

    To investigate the influence of sodium to potassium (Na/K) ratios on the growth performance and physiological response of the Pacific white shrimp ( Litopenaeus vananmei), various concentrations of KCl were added to low-salinity well water (salinity 4) in an 8-week culture trial. Six treatments with Na/K ratios of 60:1, 42:1, 33:1, 23:1, 17:1, and 14:1 were replicated in triplicate. The highest weight-gain rate (3 506±48)% and survival rate (89.38±0.88)% was observed in well water with Na/K ratios of 23:1 and 42:1, respectively, while the feed conversion ratio (1.02±0.01), oxygen consumption, and ammonia-N excretion rate was the lowest in the medium with a Na/K ratio of 23:1. Gill Na+-K+-ATPase activity, as an indicator of osmoregulation, peaked in the treatment where the Na/K ratio was 17:1. The total hemocyte count, respiratory burst, and immune-related enzyme activities (ALP, LSZ, PO, and SOD) of L. vananmei were affected significantly by Na/K ratios ( Pcontrol (75±7.07)%. In conclusion, the addition of K+ to low-salinity well water in L. vannamei cultures is feasible. Na/K ratios ranging from 23:1 to 33:1 might improve survival and growth. Immunity and disease resistance are also closely related to the Na/K ratio of the low-salinity well water. The findings may contribute to the development of more efficient K + remediation strategies for L. vananmei culture in low-salinity well water.

  7. Anti-lipopolysaccharide factor in Litopenaeus vannamei (LvALF): a broad spectrum antimicrobial peptide essential for shrimp immunity against bacterial and fungal infection.

    Science.gov (United States)

    de la Vega, Enrique; O'Leary, Nuala A; Shockey, Jessica E; Robalino, Javier; Payne, Caroline; Browdy, Craig L; Warr, Gregory W; Gross, Paul S

    2008-04-01

    Antimicrobial peptides are an essential component of the innate immune system of most organisms. Expressed sequence tag analysis from various shrimp (Litopenaeus vannamei) tissues revealed transcripts corresponding to two distinct sequences (LvALF1 and LvALF2) with strong sequence similarity to anti-lipopolysaccharide factor (ALF), an antimicrobial peptide originally isolated from the horseshoe crab Limulus polyphemus. Full-length clones contained a 528bp transcript with a predicted open reading frame coding for 120 amino acids in LvALF1, and a 623bp transcript with a predicted open reading frame coding for 93 amino acids in LvALF2. A reverse genetic approach was implemented to study the in vivo role of LvALF1 in protecting shrimp from bacterial, fungal and viral infections. Injection of double-stranded RNA (dsRNA) corresponding to the LvALF1 message resulted in a significant reduction of LvALF1 mRNA transcript abundance as determined by qPCR. Following knockdown, shrimp were challenged with low pathogenic doses of Vibrio penaeicida, Fusarium oxysporum or white spot syndrome virus (WSSV) and the resulting mortality curves were compared with controls. A significant increase of mortality in the LvALF1 knockdown shrimp was observed in the V. penaeicida and F. oxysporum infections when compared to controls, showing that this gene has a role in protecting shrimp from both bacterial and fungal infections. In contrast, LvALF1 dsRNA activated the sequence-independent innate anti-viral immune response giving increased protection from WSSV infection.

  8. Trophic transfer of lead through a model marine four-level food chain: Tetraselmis suecica, Artemia franciscana, Litopenaeus vannamei, and Haemulon scudderi.

    Science.gov (United States)

    Soto-Jiménez, M F; Arellano-Fiore, C; Rocha-Velarde, R; Jara-Marini, M E; Ruelas-Inzunza, J; Páez-Osuna, F

    2011-08-01

    The objective of this investigation was to assess the transfer of lead (Pb) along an experimental, four-level food chain: Tetraselmis suecica (phytoplankton) → Artemia franciscana (crustacean, brine shrimp) → Litopenaeus vannamei (crustacean, white shrimp) → Haemulon scudderi (fish, grunt). T. suecica was exposed to a sublethal dose of Pb in solution and then used as the base of a marine food chain. Significant differences in Pb concentrations were found between exposed organisms of the different trophic levels and the control. Particularly, Pb concentrations in fish of the simulated trophic chain were two-to three times higher in the exposed specimens than in the control. Levels of Pb in phytoplankton showed a substantial increase with respect to the solution (level I), with bioconcentration factors averaging from 930 to 3630. In contrast, a strong decrease in Pb concentration from phytoplankton to zooplankton (level II) and from zooplankton to shrimp tissues (level III) was evidenced by bioaccumulation factors metal transfer observed in these two predators, Pb concentration in the grunt fish (level IV) was higher than in the shrimp (level III) (bioaccumulation factor >1.0). Some of the added Pb is transferred from the phytoplankton along the food chain, thus producing a net accumulation of Pb mainly in fish and, to a lesser extent, in shrimp tissues. Because Pb is one of the most pervasive contaminants in coastal ecosystems, its transference by way of diet and potential net accumulation in higher predators is of ecologic importance for marine life. In addition, because shrimp and adult Haemulon scudderi are commercially important resources, this issue is of particular relevance to the safety of marine products. PMID:21082317

  9. Pacific white shrimp (Litopenaeus vannamei) vitellogenesis-inhibiting hormone (VIH) is predominantly expressed in the brain and negatively regulates hepatopancreatic vitellogenin (VTG) gene expression.

    Science.gov (United States)

    Chen, Ting; Zhang, Lv-Ping; Wong, Nai-Kei; Zhong, Ming; Ren, Chun-Hua; Hu, Chao-Qun

    2014-03-01

    Ovarian maturation in crustaceans is temporally orchestrated by two processes: oogenesis and vitellogenesis. The peptide hormone vitellogenesis-inhibiting hormone (VIH), by far the most potent negative regulator of crustacean reproduction known, critically modulates crustacean ovarian maturation by suppressing vitellogenin (VTG) synthesis. In this study, cDNA encoding VIH was cloned from the eyestalk of Pacific white shrimp, Litopenaeus vannamei, a highly significant commercial culture species. Phylogenetic analysis suggests that L. vannamei VIH (lvVIH) can be classified as a member of the type II crustacean hyperglycemic hormone family. Northern blot and RT-PCR results reveal that both the brain and eyestalk were the major sources for lvVIH mRNA expression. In in vitro experiments on primary culture of shrimp hepatopancreatic cells, it was confirmed that some endogenous inhibitory factors existed in L. vannamei hemolymph, brain, and eyestalk that suppressed hepatopancreatic VTG gene expression. Purified recombinant lvVIH protein was effective in inhibiting VTG mRNA expression in both in vitro primary hepatopancreatic cell culture and in vivo injection experiments. Injection of recombinant VIH could also reverse ovarian growth induced by eyestalk ablation. Furthermore, unilateral eyestalk ablation reduced the mRNA level of lvVIH in the brain but not in the remaining contralateral eyestalk. Our study, as a whole, provides new insights on VIH regulation of shrimp reproduction: 1) the brain and eyestalk are both important sites of VIH expression and therefore possible coregulators of hepatopancreatic VTG mRNA expression and 2) eyestalk ablation could increase hepatopancreatic VTG expression by transcriptionally abolishing eyestalk-derived VIH and diminishing brain-derived VIH.

  10. TOTAL REPLACEMENT OF FISHMEAL WITH AN ORGANICALLY CERTIFIED YEAST–BASED PROTEIN IN PACIFIC WHITE SHRIMP (Litopenaeus Vannamei DIETS: LABORATORY AND FIELD TRIALS

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    Ewen McLean

    2006-04-01

    Full Text Available The feasibility of totally replacing the fishmeal component of marine shrimp (Litopenaeus vannamei diets was examined both in the laboratory setting and during a full–scale commercial trial. Animals were fed either a traditional fishmeal–based diet or one in which complete replacement of fishmeal, on a per protein basis, was manufactured using a yeast–based product, NuPro®. Laboratory studies determined that irrespective of diet fed, no difference in shrimp performance (weight gain, survival and SGR occurred. A field trial was thus activated to determine whether lab–scale studies were transferable to the commercial setting. Trials were conducted in earthen ponds from mid–June to early November 2005. Ponds were initially stocked with PL12–16 shrimp at a rate of 100,000 per hectare. At trial end, ponds receiving the NuPro®–based feed had equivalent growth to that of shrimp fed the traditional, fishmeal–based diet. Percent increase in weight from initial values and survival for the NuPro® ponds was 296, 269 and 275%, and 78, 76 and 85% respectively, whereas that for the fishmeal–based diet was 305% and 80% respectively. Noteworthy was that within pond size variation of L. vannamei was lower in NuPro® fed animals (±2.3 g when compared against animals receiving the traditional feed (±4.1 g. Overall observations from the field trial indicate the importance of the »bioreactor« pond with respect to the supply of energy to sustain shrimp growth potential.

  11. Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection

    Institute of Scientific and Technical Information of China (English)

    LUO Zhan; ZHANG Jiquan; LI Fuhua; ZHANG Xiaojun; LIU Chengzhang; XIANG Jianhai

    2011-01-01

    Acting as one of the pattern recognition receptors (PRRs),C-type lectin is believed to mediate pathogen recognition and plays an important role in the clearance of pathogens as part of the innate immune system.In this work,a novel C-type lectin gene (named LvLecl) was cloned from the shrimp Litopenaeus vannamei.The ORF of LvLecl is 510 bp,encoding 169 amino acids.The deduced amino acid sequence contains a putative signal peptide of 19 amino acids at the N-terminal and a carbohydrate recognition domain (CRD) at the C-terminal.LvLecl was mainly expressed in the hepatopancreas.Real-time PCR analysis indicated that the level of LvLecl transcripts significantly changed in the hepatopancreas after the shrimp were artificially challenged with LPS,Micrococcus lysodeikticus and white spot syndrome virus (WSSV).RNAi-based silencing of LvLecl resulted in increases in mortality when the shrimp were challenged with WSSV,and the median lethal time was reduced compared with controls.Although there was no characteristic “EPN” (Glu-Pro-Ser) or “QPD” (Gln-Pro-Asp) motif,the recombinant LvLecl,expressed in Escherichia coli BL21 (DE3),could also agglutinate M.lysodeikticus and Vibrio anguillarum.The agglutinating activities were calcium-dependent and could be inhibited by D-mannose,D-glucose,D-galactose and N-Acetyl-D-mannose.These results suggest that LvLecl might be involved in the immune response against WSSV and bacterial infections and contribute to non-self recognition as a pattem recognition receptor in the innate immune system of the shrimp L.vannamei.

  12. Lipopolysaccharide and β-1,3-glucan-binding protein (LGBP) bind to seaweed polysaccharides and activate the prophenoloxidase system in white shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Chen, Yu-Yuan; Chen, Jiann-Chu; Kuo, Yi-Hsuan; Lin, Yong-Chin; Chang, Yu-Hsuan; Gong, Hong-Yi; Huang, Chien-Lun

    2016-02-01

    Lipopolysaccharide and β-1,3-glucan-binding protein (LGBP), important pattern recognition proteins (PRPs), recognize lipopolysaccharide (LPS) and β-1,3-glucan (βG), known as pathogen-associated molecular patterns (PAMPs), and subsequently trigger innate immunity. Several seaweed polysaccharides and seaweed extracts increase immune parameters and resistance to pathogens. Here, we constructed the expression vector pET28b-LvLGBP and transferred it into Escherichia coli BL21 (DE3) for protein expression and to produce the recombinant protein LGBP (rLvLGBP) in white shrimp Litopenaeus vannamei. We examined the binding of rLvLGBP with seaweed-derived polysaccharides including alginate, carrageenan, fucoidan, laminarin, Gracilaria tenuistipitata extract (GTE), and Sargassum duplicatum extract (SDE), and examined the phenoloxidase activity of shrimp haemocytes incubated with a mixture of rLvLGBP and each polysaccharide. We also examined the binding of rLvLGBP with LPS and βG, and the phenoloxidase activity of shrimp haemocytes incubated with a mixture of rLvLGBP and LPS (rLvLGBP-LPS) or a mixture of rLvLGBP and βG (rLvLGBP-βG). An ELISA binding assay indicated that rLvLGBP binds to LPS, βG, alginate, carrageenan, fucoidan, laminarin, GTE, and SDE with dissociation constants of 0.1138-0.1770 μM. Furthermore, our results also indicated that the phenoloxidase activity of shrimp haemocytes incubated with a mixture of rLvLGBP and LPS, βG, alginate, carrageenan, fucoidan, laminarin, GTE, and SDE significantly increased by 328%, 172%, 200%, 213%, 197%, 194%, 191%, and 197%, respectively compared to controls (cacodylate buffer). We conclude that LvLGBP functions as a PRP, recognizes and binds to LPS, βG, alginate, carrageenan, fucoidan, laminarin, GTE, and SDE, and subsequently leads to activating innate immunity in shrimp. PMID:26522339

  13. Effects of dietary chlorogenic acid on growth performance, antioxidant capacity of white shrimp Litopenaeus vannamei under normal condition and combined stress of low-salinity and nitrite.

    Science.gov (United States)

    Wang, Yun; Li, Zheng; Li, Jian; Duan, Ya-Fei; Niu, Jin; Wang, Jun; Huang, Zhong; Lin, Hei-Zhao

    2015-04-01

    An eight-week feeding trial followed by an acute combined stress test of low-salinity and nitrite were performed to evaluate effects of chlorogenic acid (CGA) on growth performance and antioxidant capacity of white shrimp Litopenaeus vannamei. Shrimp were randomly allocated in 12 tanks (30 shrimp per tank) and triplicate tanks were fed with a control diet or diets containing different levels of CGA (100, 200 and 400 mg kg(-1) feed) as treatment groups. Growth performance including weight gain (WG), biomass gain (BG), feed conversion ratio (FCR), and feed intake were determined after feeding for 56 days. Antioxidant capacity were evaluated by determining the activity of total antioxidant status (TAS), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) as well as the gene expression of GSH-Px and CAT in the hepatopancreas of shrimp at the end of feeding trial and again at the end of the combined stress test. The results indicated that supplemention of CGA had no significant effects on the growth performance and the activities of TAS, SOD, GSH-Px and CAT in hepatopancreas of shrimp cultured under normal conditions for 56 days. However, compared with the control group, CGA (200, 400 mg kg(-1) feed) significantly improved the resistance of L. vannamei against the combined stress of low-salinity and nitrite, as indicated by the significant (P CAT, as well as higher transcript levels of GPx and CAT gene in shrimp treated with CGA in the combined tress test. Our findings suggested that CGA possessed dual-modulatory effects on antioxidant capacity of L. vannamei and could be a potential feed additive that can enhance shrimp resistance against environmental stresses. The recommended application dosage is 200 mg kg(-1) and further studies are needed to clarify the action model of CGA efficiency. PMID:25600509

  14. In situ hybridization demonstrates that Litopenaeus vannamei, L. stylirostris and Penaeus monodon are susceptible to experimental infection with infectious myonecrosis virus (IMNV).

    Science.gov (United States)

    Tang, Kathy F J; Pantoja, Carlos R; Poulos, Bonnie T; Redman, Rita M; Lightnere, Donald V

    2005-02-28

    Infectious myonecrosis virus (IMNV) was recently found to be the cause of necrosis in the skeletal muscle of farm-reared Litopenaeus vannamei from northeastern Brazil. Nucleic acid extracted from semi-purified IMN virions showed that this virus contains a 7.5 kb RNA genome. A cDNA library was constructed, and a clone, designated as IMNV-317, was labeled with digoxigenin-11-dUTP and used as a gene probe for in situ hybridization (ISH). This probe specifically detected IMNV in infected tissues. To determine the susceptibility of 3 species of penaeid shrimp (L. vannamei, L. stylirostris, Penaeus monodon) to IMNV infection, juveniles were injected with purified virions and observed for clinical signs of infection and mortality over a 4 wk period. All L. vannamei exhibited typical lesions after 6 d, and lesions were visible in all L. stylirostris by Day 13. The clinical signs of opaque muscle were not seen in P. monodon, due to their highly pigmented exoskeleton precluding visual detection of lesions. Moderate mortality (20%) occurred in infected L. vannamei. No mortalities were observed in either L. stylirostris or P. monodon. Histological examination and ISH indicated that all 3 species are susceptible to IMNV infection. Using ISH, IMNV was detected in tissues including the skeletal muscle, lymphoid organ, hindgut, and phagocytic cells within the hepatopancreas and heart. In all 3 species, skeletal muscle cells produced the strongest ISH reactions. Based on the onset of clinical signs of infection and mortality, L. vannamei appears to be the most susceptible of these 3 species to IMNV infection. PMID:15819442

  15. Ontogeny of osmoregulation in the Pacific blue shrimp, Litopenaeus stylirostris (Decapoda, Penaeidae): Deciphering the role of the Na(+)/K(+)-ATPase.

    Science.gov (United States)

    Pham, Dominique; Charmantier, Guy; Boulo, Viviane; Wabete, Nelly; Ansquer, Dominique; Dauga, Clément; Grousset, Evelyse; Labreuche, Yannick; Charmantier-Daures, Mireille

    2016-01-01

    The role of the main ion transporting enzyme Na+/K+-ATPase in osmoregulation processes was investigated in Litopenaeus stylirostris. The development and localization of the osmoregulation sites were studied during ontogenesis by immunodetection of Na(+)K(+)-ATPase using monoclonal antibodies and transmission electron microscopy (TEM). Osmoregulation sites were identified as the pleurae and branchiostegites in the zoeae and mysis stages. In the subsequent post-metamorphic stages the osmoregulatory function was mainly located in the epipodites and branchiostegites and osmotic regulation was later detected in the gills. The presence of ionocytes and microvilli in these tissues confirmed their role in ionic processes. The complete open reading frame of the mRNA coding for the α-subunit of Na+K+-ATPase was characterized in L. stylirostris. The resulting 3092-bp cDNA (LsNKA) encodes a putative 1011-amino-acid protein with a predicted molecular mass of 112.3kDa. The inferred amino acid sequence revealed that the putative protein possesses the main structural characteristics of the Na+K+-ATPase α-subunits. Quantitative RT-PCR analyses indicated that LsNKA transcripts did not significantly vary between the different developmental stages. The number of transcripts was about 2.5-fold higher in the epipodites and gills than in any other tissues tested in juveniles. A reverse genetic approach was finally implemented to study the role of LsNKA in vivo. Knockdown of LsNKA expression by gene-specific dsRNA injection led to an increase of shrimp mortality following an abrupt salinity change compared to control animals. These data strongly suggest that LsNKA plays an important role in osmoregulation when the shrimp are challenged by changing salinities. PMID:26827851

  16. Differential regulation of hepatopancreatic vitellogenin (VTG) gene expression by two putative molt-inhibiting hormones (MIH1/2) in Pacific white shrimp (Litopenaeus vannamei).

    Science.gov (United States)

    Luo, Xing; Chen, Ting; Zhong, Ming; Jiang, Xiao; Zhang, Lvping; Ren, Chunhua; Hu, Chaoqun

    2015-06-01

    Molt-inhibiting hormone (MIH), a peptide member of the crustacean hyperglycemic hormone (CHH) family, is commonly considered as a negative regulator during the molt cycle in crustaceans. Phylogenetic analysis of CHH family peptides in penaeidae shrimps suggested that there is no significant differentiation between MIH and vitellogenesis-inhibiting hormone (VIH, another peptide member of CHH family), by far the most potent negative regulator of crustacean vitellogenesis known. Thus, MIH may also play a role in regulating vitellogenesis. In this study, two previously reported putative MIHs (LivMIH1 and LivMIH2) in the Pacific white shrimp (Litopenaeus vannamei) were expressed in Escherichia coli, purified by immobilized metal ion affinity chromatography (IMAC) and further confirmed by western blot. Regulation of vitellogenin (VTG) mRNA expression by recombinant LivMIH1 and LivMIH2 challenge was performed by both in vitro hepatopancreatic primary cells culture and in vivo injection approaches. In in vitro primary culture of shrimp hepatopancreatic cells, only LivMIH2 but not LivMIH1 administration could improve the mRNA expression of VTG. In in vivo injection experiments, similarly, only LivMIH2 but not LivMIH1 could stimulate hepatopancreatic VTG gene expression and induce ovary maturation. Our study may provide evidence for one isoform of MIH (MIH2 in L. vannamei) may serve as one of the mediators of the physiological progress of molting and vitellogenesis. Our study may also give new insight in CHH family peptides regulating reproduction in crustaceans, in particular penaeidae shrimps.

  17. Polyculture of white shrimp, Litopenaeus vannamei and milkfish, Chanos chanos as a strategy for efficient utilization of natural food production in ponds

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    Bessie Joy G. Elle

    2011-12-01

    Full Text Available Both shrimps and milkfish are important aquaculture species in the Philippines. In the presentstudy, we described a modified extensive polyculture method for white shrimp, Litopenaeus vannameiand miklkfish, Chanos chanos in ponds during the wet months. This particular culture system uses two ormore species in a particular production area with the purpose of maximizing the utilization of theavailable natural food present in the system. Two 1-hectare earthen ponds were prepared and addedwith organic and/or inorganic fertilizers to enhance natural food production. The ponds were stocked withwhite shrimp postlarvae (PL at a density of 1 PL m-2 and milkfish at 750 juveniles ha-1. There was nowater exchange during the first two months of culture followed by a bi-weekly water exchange untilharvest. No artificial feeding was provided during the culture period, instead, the ponds weresupplemented with inorganic fertilizers every month to ensure stable production of natural food. Bothshrimps and milkfish appeared healthy and no disease outbreaks were observed during the productioncycle. The physico-chemical parameters of the water in the ponds were within the optimum levels thatare required for both shrimp and milkfish farming. The phytoplankton population was stable andpredominantly composed of Chlorella sp., a green microalga (Chlorophyta. The cultured stock wereharvested after 100 days of culture at sizes of 13-15 for white shrimp and > 250 g for milkfish. Therewas high survival rate of milkfish but low for white shrimps, and this could be affected by the prevailingclimatic condtions during culture and the age and size of the species during stocking.

  18. Activating transcription factor 4 and X box binding protein 1 of Litopenaeus vannamei transcriptional regulated white spot syndrome virus genes Wsv023 and Wsv083.

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    Xiao-Yun Li

    Full Text Available In response to endoplasmic reticulum (ER stress, the signaling pathway termed unfolded protein response (UPR is activated. To investigate the role of UPR in Litopenaeus vannamei immunity, the activating transcription factor 4 (designated as LvATF4 which belonged to a branch of the UPR, the [protein kinase RNA (PKR-like ER kinase, (PERK]-[eukaryotic initiation factor 2 subunit alpha (eIF2α] pathway, was identified and characterized. The full-length cDNA of LvATF4 was 1972 bp long, with an open reading frame of 1299 bp long that encoded a 432 amino acid protein. LvATF4 was highly expressed in gills, intestines and stomach. For the white spot syndrome virus (WSSV challenge, LvATF4 was upregulated in the gills after 3 hpi and increased by 1.9-fold (96 hpi compared to the mock-treated group. The LvATF4 knock-down by RNA interference resulted in a lower cumulative mortality of L. vannamei under WSSV infection. Reporter gene assays show that LvATF4 could upregulate the expression of the WSSV gene wsv023 based on the activating transcription factor/cyclic adenosine 3', 5'-monophosphate response element (ATF/CRE. Another transcription factor of L. vannamei, X box binding protein 1 (designated as LvXBP1, has a significant function in [inositol-requiring enzyme-1(IRE1 - (XBP1] pathway. This transcription factor upregulated the expression of the WSSV gene wsv083 based on the UPR element (UPRE. These results suggest that in L. vannamei UPR signaling pathway transcription factors are important for WSSV and might facilitate WSSV infection.

  19. The anesthetic efficacy of eugenol and the essential oils of Lippia alba and Aloysia triphylla in post-larvae and sub-adults of Litopenaeus vannamei (Crustacea, Penaeidae).

    Science.gov (United States)

    Parodi, Thaylise V; Cunha, Mauro A; Heldwein, Clarissa G; de Souza, Diego M; Martins, Átila Clivea; Garcia, Luciano de O; Wasielesky, Wilson; Monserrat, José María; Schmidt, Denise; Caron, Braulio O; Heinzmann, Berta; Baldisserotto, Bernardo

    2012-04-01

    The aim of this study was to evaluate the anesthesia induction and recovery times of sub-adult and post-larvae white shrimp (Litopenaeus vannamei) that were treated with eugenol and the essential oils (EOs) from Lippia alba and Aloysia triphylla. Oxidative stress parameters in the hemolymph of this species were also analyzed. The concentrations of eugenol, A. triphylla EO and L. alba EO recommended for anesthesia were 200, 300 and 750 μL L(-1) for sub-adults and 175, 300 and 500 μL L(-1) for post-larvae, respectively. The concentrations studied during the transport of sub-adults were between 20 and 50 μL L(-1) eugenol, 20-30 μL L(-1)A. triphylla EO and 50 μL L(-1)L. alba EO. For post-larvae, the optimal concentrations for transport were 20 μL L(-1) eugenol and between 20 and 50 μL L(-1)A. triphylla EO. The white shrimp sub-adults that were exposed to A. triphylla EO (20 μL L(-1)) showed increases in their total antioxidant capacities (150%), catalase (70%) and glutathione-S-transferase (615%) activity after 6 h. L. alba EO (50 μL L(-1)) and eugenol (20 μL L(-1)) also increased GST activity (1292 and 1315%) after 6 h, and eugenol (20 μL L(-1)) decreased the total antioxidant capacity (100%). Moreover, concentrations above 30 μL L(-1) for the EOs of A. triphylla and L. alba and 20 μL L(-1) eugenol were effective at inducing anesthesia and improving the antioxidant system against reactive oxygen species (ROS) after 6 h. PMID:22198554

  20. Dietary supplementation of short-chain fructooligosaccharides influences gastrointestinal microbiota composition and immunity characteristics of Pacific white shrimp, Litopenaeus vannamei, cultured in a recirculating system.

    Science.gov (United States)

    Li, Peng; Burr, Gary S; Gatlin, Delbert M; Hume, Michael E; Patnaik, Susmita; Castille, Frank L; Lawrence, Addison L

    2007-12-01

    Supplementation of prebiotic compounds, including short-chain fructooligosaccharides (scFOS) has been shown to confer benefits on nutrient utilization, growth, and disease resistance of various animal species through improved gastrointestinal (GI) microbiota. However, potential uses of prebiotics for shrimp have not been defined. A 6-wk feeding trial was conducted in a recirculating system to determine the effects of scFOS supplementation on growth performance, immune functions, and GI microbiota composition of Pacific white shrimp (Litopenaeus vannamei). scFOS was supplemented in a nutritionally complete diet (35% crude protein) at 0.025, 0.0500, 0.075, 0.100, 0.200, 0.400, and 0.800% by weight. After 6 wk of feeding, shrimp fed 0, 0.1, and 0.8% scFOS were sampled for assays of immune function and GI microbiota. Dietary supplementation of scFOS did not improve weight gain, feed conversion ratio, or survival of shrimp. Denaturing gradient gel electrophoresis analysis suggested the intestinal tract microbial community from shrimp fed the basal diet was different from that of shrimp fed the scFOS diets [similarity coefficient (SC) = 74.9%)], although the intestinal tract microbial community from shrimp fed the scFOS-supplemented diets was very similar (SC = 92.3%). All the bacterial species contributing to the GI microbial differences were identified, although most of them are uncultured species. Both total hemocyte count and hemocyte respiratory burst increased (P < 0.05) by incremental dietary supplementation of scFOS (0-0.8%). This study is the first to our knowledge to show that dietary scFOS can selectively support growth of certain bacterial species in the GI tract of shrimp and enhance immunity, which may facilitate development of alternative strategies, including novel probiotics and synbiotics, for shrimp growth and health management. PMID:18029496

  1. AVALIAÇÃO DO GANHO DE PESO DE PÓS-LARVAS DO CAMARÃO MARINHO Litopenaeus vannamei (BOONE, 1931, ALIMENTADOS COM PEIXES DA FAUNA ACOMPANHANTE DO CAMARÃO MARINHO EVALUATION OF WEIGHT GAIN IN POST-LARVAE OF MARINE SHRIMP Litopenaeus vannamei (BOONE, 1931 FED ON FISH SPECIES WHICH COMPOSE THE MARINE SHRIMP FISHERIES BYCATCH

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    Henrique José Mascarenhas dos Santos Costa

    2007-04-01

    Full Text Available O objetivo do presente trabalho foi analisar o desenvolvimento inicial de pós-larvas de Litopenaeus vannamei em água doce utilizando como alimento peixes da fauna acompanhante da pesca do camarão marinho. Cultivaram-se 48 pós-larvas com peso e comprimento médio total iniciais de 0,008±0,001 g e 11,0±0,5 mm, respectivamente. Para cada tratamento foram utilizados, como alimento, ração comercial (RC para camarão marinho com 45% de proteína bruta, Opisthonema oglinum (OO (sardinha bandeira e Chloroscombus chrysurus (CC (palombeta, respectivamente. Utilizou-se o delineamento de blocos ao acaso com quatro repetições. A densidade de estocagem consistiu de quatro camarões por aquário. Durante os 75 dias de experimento, a alimentação foi administrada ad libitum. Registrou-se a temperatura média final de 27,1±0,8ºC para os três tratamentos e o pH médio final de 7,68±0,27 para os camarões alimentados com RC e 7,66±0,22 para os indivíduos alimentados com OO e CC, respectivamente. Os pesos médios finais foram de 0,560±0,096 g, 0,495±0,091 g e 0,500±0,101 g e os comprimentos totais finais de 62,1±0,9 mm, 57,0±0,8 mm e 56,2±0,9 mm para os tratamentos com RC, OO e CC, respectivamente. Os resultados do peso e comprimento final mostraram que não houve diferença estatística significativa entre os tratamentos testados. A sobrevivência final foi de 87,5±14,4%, 68,8±12,5% e 62,5±14,3% para o RC, OO e CC, respectivamente. Conclui-se que a utilização de ração para camarão marinho e os peixes marinhos O. oglinum e C. chrysurus como alimento para pós-larvas de camarão marinho apresentam desenvolvimento semelhante. PALAVRAS-CHAVE: Camarão marinho, dietas, Chloroscombus chrysurus, Litopenaeus vannamei, Opisthonema oglinum. The objective of this study was to evaluate the early development of Litopenaeus vannamei post-larvae in freshwater fed on fish species that compose the shrimp fisheries bycatch. Forty eight post

  2. Evaluación de la Presencia de las Enfermedades de Newcastle y Gumboro en Aves Silvestres en Explotaciones Avícolas de Fómeque‚ Cundinamarca -resumen-

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    Laura V Vargas-Castillo

    2014-12-01

    Full Text Available En Colombia‚ se ha reportado la circulación de cepas de enfermedades virales en aves de corral‚ lo cual ha generado preocupación en la industria avícola‚ permitiendo un espacio para la evaluación de las estrategias de prevención y control‚ y de vigilancia epidemiológica en zonas de alta concentración de aves de corral y a la posible transmisión de dichas enfermedades entre las especies. El objetivo de este estudio fue evaluar la circulación de los virus de la enfermedad de Newcastle (Paramyxoviridae‚ Avulavirus y enfermedad de Gumboro (Birnaviridae‚ Birnavirus mediante técnicas serológicas (ELISA y moleculares (RT-PCR‚ identificar las aves silvestres en la zona y establecer la percepción del contacto de éstas con las aves de corral. La presencia de los animales se estableció dentro y adyacente a nueve granjas del municipio de Fómeque‚ Cundinamarca‚ entre 2012 y 2013‚ mediante observación‚ registro fotográfico y captura de individuos; además‚ se llevó a cabo la aplicación de una encuesta con ilustraciones a trabajadores y propietarios identificando el contacto con las aves de corral e instalaciones‚ en el lugar donde fueron observadas. Se identificaron 6 órdenes‚ 17 familias‚ 30 géneros‚ 32 especies y 417 individuos‚ de los cuales en su captura se obtuvieron tres órdenes‚ Passeriformes con el mayor número‚ seguido por Columbiformes y en menor número del orden Apodiformes con tres especies‚ siendo muestreados un total de 48 aves en estrecho contacto con las aves de corral; a su vez‚ los datos suministrados por los trabajadores y propietarios afirmaron la presencia en su mayoría de aves de pequeño a mediano tamaño cerca a la bodegas de almacenamiento‚ sobre las tejas o en árboles frutales‚ y aves de mayor tamaño sobrevolando las granjas. Todas las muestras fueron negativas para la enfermedad de Newcastle (NDV y la enfermedad de Gumboro (IBDV mediante la técnica RT

  3. Caracterización de sitios de percha del guajolote silvestre (Meleagris gallopavo mexicana en Sierra Fría, Aguascalientes, México Roost sites characteristics of wild turkey (Meleagris gallopavo mexicana in Sierra Fria, Aguascalientes, Mexico

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    Marcelo Márquez-Olivas

    2007-06-01

    Full Text Available Entre octubre y noviembre del año 2001 y mayo de 2002 se localizaron 16 sitios de percha de guajolote silvestre (Meleagris gallopavo mexicana en la Sierra Fría de Aguascalientes. El 87.5% de los sitios se encontraron en bosques de pino-encino (en rodales de pinos, en altitudes de 2500 a 2650 m. En la parte alta (2/3 de la cuesta se localizaron 8 de los sitios y 7 sobre laderas de exposición noreste, en una pendiente promedio de 38.9 ± 12.6%. El 62.5% de los sitios se usaron durante todo el periodo de estudio y el resto, de manera ocasional. La mayoría de los dormideros se encontraron cerca de comederos o cebaderos y de parcelas agrícolas. Se contabilizaron de 1 a 23 árboles de percha utilizados por sitio, distribuidos a una distancia promedio de 24.4 ± 17.8 metros. El 81.2% de los árboles (n=170 fueron pinos (Pinus teocote, P. durangensis, P. leiophylla y P. michoacana, 17.6% encinos (Quercus laurina, Q. grisea y Q. eduardii y 1.2% madroños (Arbutus arizonica. La altura promedio de los árboles fue de 16.6 ± 4.4 m y el diámetro 43.2 ± 11.5 cm. La preferencia del guajolote silvestre por utilizar pinos como dormideros en lugar de otras especies arbóreas, se debe posiblemente a que son árboles de mayor porte o a la conformación de su dosel; sin embargo, se ha observado que en lugares donde hay pocos pinos, los guajolotes utilizan para dormir otros árboles de características semejantes, como encinos, madroños o sicomoros.Sixteen roosting sites of Gould's wild turkey (Meleagris gallopavo mexicana were found between October and November of 2001 and May of 2002 in Sierra Fría, Aguascalientes. A large percentage (87.5% of the roost sites was located in oak-pine forest (in pine stands, at an elevation range of 2500 to 2650 meters. Eight roost sites were on the upper portion (2/3 of the ridge, and 7 at northeast exposures with an average slope of 38.9 ± 12.6%. Most (81.2% of the roost trees (n =170 were pines (Pinus teocote, P

  4. Jungle yellow fever: clinical and laboratorial sudies emphasizing viremia on a human case Febre amarela silvestre: estudo clínico e laboratorial, enfatizando a viremia, de um caso humano

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    Elza da S. Nassar

    1995-08-01

    Full Text Available The authors report the clinical, laboratorial and epidemiological aspects of a human case of jungle yellow fever. The patient suffered from fever, chills, sweating, headaches, backaches, myalgia, epigastric pains, nausea, vomiting, diarrhea and prostration. He was unvaccinated and had been working in areas where cases of jungle yellow fever had been confirmed. Investigations concerning the yellow fever virus were performed. Blood samples were collected on several days in the course of the illness. Three of these samples (those obtained on days 5,7 and 10 were inoculated into suckling mice in attempt to isolate virus and to titrate the viremia level. Serological surveys were carried out by using the IgM Antibodies Capture Enzyme Linked Immunosorbent Assay (MAC-ELISA, Complement Fixation (CF, Hemagglulinalion Inhibition (HI and Neutralization (N tests. The yellow fever virus, recovered from the two first samples and the virus titration, showed high level of viremia. After that, specific antibodies appeared in all samples. The interval between the end of the viremia and the appearance of the antibodies was associated with the worsening of clinical symptoms, including bleeding of the mucous membrane. One must be aware of the risk of having a urban epidemics in areas where Aedes aegypti is found in high infestation indexes.Os autores estudaram um caso humano de febre amarela silvestre, sob os aspectos clínico, laboratorial e epidemiológico. O paciente apresentava febre (39ºC, calafrios, sudorese, cefaléia, dor lombar, mialgia, dor abdominal em epigástrio, náuseas, vômitos, diarréia e prostração. Relatava permanência em área onde foram constatados casos de febre amarela silvestre e não havia histórico de vacinação anterior. Frente às suspeitas que levaram à investigação do vírus da febre amarela, foram colhidas várias amostras de sangue no curso da doença. As amostras do 5º, 7º e 10º dias foram submetidas a provas de

  5. EVALUACIÓN In Situ DE LA VARIABILIDAD GENÉTICA DE LOS CHILES SILVESTRES (Capsicum spp. EN LA REGIÓN FRAILESCA DEL ESTADO DE CHIAPAS, MÉXICO

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    R. A. Alonso

    2008-01-01

    Full Text Available Se realizó un estudio con el objetivo de hacer la evaluación in situ de la variabilidad genética de los chiles silvestres en la región Frailesca del estado de Chiapas. La muestra experimental se constituyó con el 10 % de las comunidades y productores de cada municipio. La unidad de muestreo fue el sitio donde se encontraron las plantas. La evaluación in situ se basó en las características cuantitativas y cualitativas contenidas en el descriptor de Capsicum. La variabilidad se midió utilizando los índices de diversidad de Margalef, Simpson y Sorensen. Los caracteres cuantitativos con mayor valor discriminante fueron: número de flores por axila, longitud del filamento, diámetro, longitud y peso de los frutos, longitud de la placenta, número y peso de 1000 semillas. Como resultado del análisis discriminante, se formaron tres grupos: el grupo 1, donde predominan los Capsicum annuum, con seis accesiones que poseen características de C. frutescens; el grupo 2, integrado por las accesiones de Capsicum frutescens, y el grupo 3, con los C. annuum cultivados. Se evidenció la existencia de altos niveles de variabilidad en las muestras de chile evaluadas, las cuales integraron tres grupos con características que identifican las especies: Capsicum frutescens L., Capsicum annuum L. y 12 accesiones cultivadas de Capsicum annuum. La mayor variabilidad se encontró en los municipios La Concordia y Villaflores, existiendo condiciones para la conservación de la variabilidad in situ.

  6. Preferencias en la anidación de tortugas carey (Eretmochelys imbricata y baulas (Dermochelys coriacea en el Refugio Nacional de Vida Silvestre Gandoca Manzanillo, Limón, Costa Rica

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    Lilliana Piedra-Castro

    2015-09-01

    Full Text Available El Caribe costarricense es importante como sitio de alimentación, copulación, anidación y desove de las tortugas marinas, por lo que el presente trabajo pretendió actualizar el patrón de anidación en las tortugas marinas Dermochelys coriacea y Eretmochelys imbricata en el sector Gandoca, del Refugio Nacional de Vida Silvestre Gandoca Manzanillo, Limón, Costa Rica, para proponer medidas de manejo. Se realizaron recorridos nocturnos entre las 8:00 p. m. y 4:00 a. m., la playa se dividió en cinco sectores, en estos se realizaron observaciones directas y de rastros, de las tortugas que elaboraron el nido, entre febrero y agosto del 2011 y 2012. Se registraron tres especies de tortugas marinas, Dermochelys coriacea, Eretmochelys imbricata y Chelonia mydas. La anidación de tortugas baula tuvo su punto máximo entre marzo y julio; por su parte, las carey, entre mayo y junio (2011, y junio hasta agosto en el 2012. Se observó preferencia en la posición de la playa utilizada para construir sus nidos; la baula utiliza principalmente la parte media, mientras que la carey utilizó mayormente la parte baja para anidar (t = 17.2525. Se observó que la baula utilizó frecuentemente el sector C; sin embargo, no se encontraron diferencias en el uso de los sectores. Se concluye que ambas especies tienen preferencias en la selección de la zona de playa en que anidan; la carey en la parte baja cerca de la línea de costa y la baula en la parte media. Además, las tortugas baula utilizan indistintamente los diferentes sectores de la playa estudiada.

  7. Diseño y Evaluación de Elementos de Enriquecimiento Ambiental para el titi gris (Saguinus leucopus en proceso de Rehabilitación, en el Centro de Rehabilitacion de Fauna Silvestre del Oriente de Caldas (CRFSOC

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    Esperanza Sepúlveda-Rojas

    2014-12-01

    Full Text Available El Enriquecimiento Ambiental se realiza en todo proceso de rehabilitación con el fin de adaptar al animal a un medio similar al que encontrará en el momento de la liberación y así mismo reducir el estrés del cautiverio y ofrecer una mejor calidad de vida, para este fin se diseñó y evaluó la efectividad de elementos de enriquecimiento ambiental para una pareja de titi gris (Saguinus leucopus en el Centro de Rehabilitación de Fauna Silvestre del Oriente de Caldas. Primero se realizo la observación preliminar de los individuos, luego se utilizaron los métodos de animal focal con registro continuo para el catalogo comportamental previo al enriquecimiento y animal focal con registro instantáneo para la evaluación de los enriquecedores ambientales. En comparación con el catalogo previo se registraron 14 nuevos comportamientos para el macho, 4 asociados, 10 no asociados y 9 nuevos comportamientos para la hembra , 4 asociados y 5 no asociados, presentando mayores frecuencias las categorías de alimentación y exploración en ambos casos. Los enriquecedores más usados fueron Vara con frutos en vaina y Caja de forrajeo. Se calcularon los índices de Shannon semanales para cada individuo registrándose incremento gradual para ambos de la semana 1 a la 4, lo cual demostró aumento en la diversidad de comportamientos, siendo estos estimulados por los enriquecedores ambientales que se implementaron. La implementación de los enriquecedores se considera exitosa ya que los objetivos se cumplieron, por lo cual concluimos que con este enriquecimiento ambiental se estimulan los comportamientos naturales necesarios para llevar a cabo una rehabilitación exitosa.

  8. Evaluación y valorización económica de la fauna silvestre en el río Algodón, Amazonía peruana

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    Rolando Aquino

    2013-04-01

    Full Text Available El presente trabajo informa sobre los hábitats, abundancia cuantitativa y valorización económica de la fauna silvestre que habitan en la cuenca del río Algodón. El estudio está basado en la diferenciación de hábitats, entrevistas a cazadores y censos por transecto. En 7 de los 10 tipos de hábitats diferenciados fueron realizados censos, donde ocurrieron en total 327 avistamientos directos de mamíferos, aves y reptiles. Entre los mamíferos, el pecarí labiado (Tayassu pecari Link y el choro (Lagothrix lagotricha Humboldt fueron los más abundantes con 9,8 individuos/km2, equivalente a 323,4 kg/km2 de biomasa, y 18,4 individuos/km2 equivalente a 202,4 kg/km2 de biomasa, respectivamente. En aves, la mayor densidad correspondió a la pucacunga (Penelope jacquacu Spix con 5,4 individuos/km2, equivalente a 6,9 kg/km2 de biomasa. Sin considerar las especies nocturnas y algunas diurnas, en el área de estudio se estiman alrededor de 31891 animales de caza entre mamíferos y aves, cuyo valor económico fue estimado en US$ 349374. De ellos, 3479 individuos tienen el valor de comercial y pertenecen a cuatro especies de ungulados y uno de aves, el resto tienen el valor de subsistencia.

  9. Diversidade de helmintos intestinais em mamíferos silvestres e domésticos na Caatinga do Parque Nacional Serra da Capivara, Sudeste do Piauí, Brasil Diversity of wild and domestic mammal's intestinal helminths from the Caatinga of the Parque Nacional Serra da Capivara, Southeast of Piauí, Brazil

    OpenAIRE

    Brandão Martha Lima; Marcia Chame; José Luis Passos Cordeiro; Sérgio Augusto de Miranda Chaves

    2009-01-01

    Estudos sobre biodiversidade permitem avaliações ecossistêmicas e monitoramentos de impactos e mudanças ambientais. A diversidade parasitária pode refletir o processo coevolutivo entre parasitos e hospedeiros, e mudanças ambientais que permitem a perda, ganho ou a manutenção das espécies nesse processo. O presente trabalho utilizou espécies/morfotipos de ovos de helmintos encontrados em fezes de sete mamíferos silvestres (os grupos Dasypodidae e grandes felinos, e as espécies: Tamandua tetrad...

  10. Contenido de alimento y metabolismo ceco-cólico en el tracto digestivo de poblaciones silvestres de iguana negra (Ctenosaura pectinata) en Morelos, México Cecum-colic content of food and metabolism in the digestive tract of wild population black lizard (Ctenosaura pectinata) in Morelos, Mexico

    OpenAIRE

    L Vélez-Hernández; MA Cobos-Peralta; JL Arcos-García

    2012-01-01

    Se colectaron cinco tractos digestivos de iguana negra (Ctenosaura pectinata) en estado silvestre en el estado de Morelos, México, para conocer la composición botánica de la dieta y el metabolismo ceco-cólico. Se observaron frotis realizados del contenido ceco-cólico para registrar la presencia de protozoarios, bacterias y parásitos intestinales existentes. Se obtuvo la media y desviación estándar de las características evaluadas. En el contenido ceco-cólico se identificaron las especies vege...

  11. RESCATE Y MANEJO DE FAUNA SILVESTRE EX SITU EN COLOMBIA: ESTUDIO DE CASO DE UN JAGUAR (PANTHERA ONCA)EN LA ORINOQUÍA COLOMBIANA / MANAGEMENT AND RESCUE OF WILDLIFE EX SITU IN COLOMBIA: A STUDY CASE OF A JAGUAR (PANTHERA ONCA) IN THE COLOMBIAN ORINOQUIA

    OpenAIRE

    Zarrate-Charry, Diego; Laverde Trujillo, Laura M.; Balaguera-Reina, Sergio A.; González-Maya, Jose F.; Trujillo, Fernando

    2009-01-01

    La caza y comercialización de fauna silvestre en Colombia es un problema de amplias proporciones, debido principalmente a la magnitud en la cual se presenta dentro del territorio nacional, siendo catalogada como el tercer negocio de carácter ilegal más rentable del país después del narcotráfi co y la venta de armas. Esta situación hace que los pobladores de zonas rurales y urbanas utilicen este tipo de fauna como método de subsistencia, llevando las poblaciones naturales a situaciones preo...

  12. Análise cromatográfica de ácidos orgânicos em cogumelos silvestres comestíveis do Nordeste de Portugal: validação de uma técnica de UFLC-PDA

    OpenAIRE

    Barros, Lillian; Pereira, Carla; Isabel C. F. R. Ferreira

    2012-01-01

    Na Península Ibérica as verduras (silvestres ou tradicionalmente cultivadas) têm sido alimentos importantes na dieta humana. O valor nutricional de verduras e os seus benefícios para a saúde têm sido reconhecidos como importantes alvos de investigação. Os grelos, inflorescências da couve-nabo (Brassica napus L. var. napus) e os espigos, inflorescências da couve-tronchuda (Brassica oleracea L. var. costata DC.) são exemplos de espécies tradicionalmente cultivadas e amplamente consumidas nas re...

  13. Evaluación in vivo del efecto cicatrizante de un gel a base de quitosano obtenido de exoesqueleto de camarón blanco Litopenaeus vannamei

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    Heimy Franceline Martínez Sánchez

    2014-06-01

    Full Text Available Healing effect of a chitosan-based gel obtained from the exoskeleton of white shrimp Litopenaeus vannamei and its evaluation in vivoTítulo corto: Evaluación del quitosano como cicatrizanteResumen:  En México alrededor del 62 % de la población sufre de accidentes causantes de alteraciones en la piel como quemaduras, heridas y diabetes principalmente. Para atender esta problemática, se propone el uso de un gel de quitosano, obtenido del exoesqueleto de camarón ya que presenta actividad antimicrobiana e inmunológica acelerando la cicatrización. Se evaluó el efecto sinérgico sobre la velocidad de cicatrización, aplicándolo en heridas de 1 cm2 infringidas a 48 ratones albinos, agrupados en cuatro tratamientos; Quitosano 0.15 y 0.30 %, producto cicatrizante (Ketanserina al 2 % y blanco (testigos sin tratamiento. El tiempo para la cicatrización sin tratamiento y el producto comercial fue 14 días, teniendo el control un efecto cicatrizante del 0 %, mientras que los geles de quitosano 0.15 y 0.30 % cicatrizaron en 7 días (P> 0.05 con efecto cicatrizante del 58 % para el quitosano 0.15 y 64 % para el quitosano 0.30.Palabras clave: piel, heridas, gel, quitosano, cicatrización.Abstract: Skin problems in Mexico have steadily increased (62 % by burns, wounds and diabetes mainly. To solve this problem, chitosan can be implemented; this polysaccharide has an antimicrobial and immunology activity against bacteria and fungi, accelerating healing. Therefore, synergistic effect of chitosan gel on the rate of healing, obtained from the exoskeleton of shrimp was evaluated. This was tested by applying it 1 cm2 over inflicted wounds of 48 albino mice, of 45 days old and between 23 to 26 g of weight, during 14 days, and grouped into four treatments: 1 0.15 % chitosan 2 0.30 % chitosan, 3 commercial product 2 % Ketanserin and 4 A batch of untreated controls. The time required for healing without treatment was 14 days, and with the utilization of 0

  14. Influência de duas dietas na qualidade da água dos tanquesberçário, utilizados no cultivo do camarão marinho Litopenaeus vannamei (Boone, 1931

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    Adriano Prysthon da Silva

    2006-01-01

    Full Text Available Cultivos experimentais com pós-larvas do Litopenaeus vannamei, na fase de berçário, foram realizados em uma fazenda de camarão, objetivando avaliar a influência da utilização de duas dietas na qualidade físico-química das águas residuais. As dietas foram formuladas à base de ração comercial e náuplios de artêmia, denominadas MAC e MAA, respectivamente. Foram utilizadas pós-larvas no estágio (PL19, estocadas a 16PL/L em dois tanques-berçário de 60 m3. Ao final de 10 dias de cultivo, as concentrações dos compostosnitrogenados (amônia, nitrito e nitrato e fosfatados (fósforo total e ortofosfatos dos tanquesberçário foram menores na dieta MAA do que na MAC (pExperiments were carried out in a commercial marine shrimp farm in order to evaluate the use of two diets in Litopenaeus vannamei post-larvae, during nursery phase, on physiochemical wastewater quality. Two feeding methods were adopted: Commercial Feeding Method (MAC and Artemia Feeding Method (MAA. The nursery tanks (60 m3 were provided with 19 days post-larvae (PL19 with a stocking density of 16PL/liter. After 10 days of culture, the concentrations of nitrogen (ammonia, nitrite and nitrate and phosphorus compounds (total phosphorus and orthophosphate in the nursery tank were smaller for MAC, rather than MAA treatment (p<0.05. Consequently, MAA treatment induced low levels of daily compounds increment,thus contributing to a significant reduction of the nutrients that caused water eutrophication.

  15. The impact of oxygen consumption by the shrimp Litopenaeus vannamei according to body weight, temperature, salinity and stocking density on pond aeration: a simulation = Impacto do consumo de oxigênio do camarão Litopenaeus vannamei em relação ao peso corporal, temperatura, salinidade na aeração do viveiro: uma simulação

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    Luis Vinatea

    2011-04-01

    Full Text Available A simulation was conducted to determinate the impact caused by thecombination of Litopenaeus vannamei respiratory rate (mg O2 shrimp-1 h-1, the behavior of SOTR (kg O2 h-1 of mechanical aerators as a function of salinity, as well as the oxygen consumption rate of the pond water and soil (mg O2 L-1 h-1 on the aeration of shrimp ponds (1, 10, 50 and 100 ha stocked with different densities (10, 40 and 120 shrimp m-2, salinities (1, 13, 25 and 37 ppt, temperatures (20, 25 and 30°C, and shrimp wet weight (5, 10, 15 and 20 g. Results showed that under lower salinity, with larger shrimp, and higher stocking density, higher will be the quantity of required 2-HP aerators to keep dissolved oxygen over 50% saturation. In addition, under low salinity, with 5 and 10 g shrimp, independent of stocking density, more aerators per hectare are required and electricity cost is higher at 20°C and salinity 1 ppt. Less aerators and lower electricity cost was observed at 30°C, salinities of 25 and 37 ppt, and shrimp of 15 and 20 g. Baseado em estudos de respiração de Litopenaeus vannamei (mg O2 camarão-1 h-1, comportamento do Standard Oxygen Transfer Rate (SOTR, kg O2 h-1 de aeradores mecânicos em função da salinidade, assim como as taxas de respiração da água e do solo (mg O2 L-1 h-1, uma simulação foi realizada a fim de determinar o impacto que estas três variáveis juntas têm sobre a aeração de viveiros (1, 10, 50 e 100 ha, estocados com diferentes densidades (10, 40 e 120 camarões m-2 em salinidades de 1, 13, 25 e 37 ppm, temperaturas de 20, 25 e 30°C e peso úmido dos camarões de 5, 10, 15 e 20 g. Os resultados mostraram que em salinidades mais baixas, com animais maiores e maiores densidades de estocagem, maior será a quantidade de aeradores de 2 cvnecessários para manter o oxigênio dissolvido acima de 50% da saturação. Igualmente, em baixas salinidades e com camarões de 5 e 10 g, independente da densidade de estocagem, mais aeradores

  16. 凡纳滨对虾(Litopenaeus vannamei) F0-ATP合酶b链全长cDNA的克隆及组织分布%cDNA Cloning and Study on Tissue Distribution of F0-ATP Synthase b-chain ofLitopenaeus vannamei

    Institute of Scientific and Technical Information of China (English)

    何晓东; 刘庆慧; 关广阔; 李倩; 李晨; 黄倢

    2015-01-01

    采用RACE方法克隆得到了凡纳滨对虾(Litopenaeus vannamei)的F0-ATP合酶b链基因的全长cDNA序列.生物信息学分析显示,该基因开放阅读框744 bp,编码248个氨基酸,分子量为28.2 kDa.Blast比对结果显示,克隆得到的cDNA序列所编码的氨基酸序列与海虱(Caligus clemensi) F0-ATP合酶β亚基的同源性为50%,与黑腹果蝇(Drosophila melanogaster) F0-ATP合酶β亚基的同源性为60%.免疫组化实验及流式细胞分析表明,F0-ATP合酶b链广泛分布于对虾鳃组织中,并且在对虾血细胞表面有分布.%White spot syndrome virus (WSSV) is a major fatal pathogen to shrimp. It is known that the b-chain of F0-ATP synthase plays a key role in the synthesis of ATP in all living organisms. Evidence from our previous research indicated that the b-chain of F0-ATP synthase ofLitopenaeus vannamei was involved in WSSV infection. However the full-length sequence of the b-chain of F0-ATP synthase in L. vannamei has not been available yet. In this study we cloned the full cDNA using reverse transcription PCR (RT-PCR) and the rapid amplification of cDNA ends (RACE) method. Bioinformatics analysis was performed to predict the amino acid sequence and the secondary and space structure of the b-chain of F0-ATP synthase. We also mapped the homology and phylogenic tree using ClustalX 1.83 and MEGA 4.02. Immuno-histochemical and flow cytometry analysis were carried out to detect the tissue distribution of the b-chain of F0-ATP synthase in L. vannamei. The results showed that the 1129 bp full length cDNA was successfully cloned. Bioinformatics analysis indicated that the full length cDNA had an open reading frame (ORF) of 744 bp that encoded 248 amino acids, and that the predicted molecular weight of the mature peptide was 28.2 kDa. The homology analysis of the b-chain of F0-ATP synthase between species demonstrated that there was a higher similarity betweenL. vannamei andCaligus clemensi (50%), and Drosophila melanogaster

  17. Efeito do tipo de sedimento na eficiência alimentar, crescimento e sobrevivência de Litopenaeus vannamei (Boone, 1931 - doi: 10.4025/actascibiolsci.v33i4.6134 Effect of sediment type on feed efficiency, growth and survival of Litopenaeus vannamei (Boone, 1931 - doi: 10.4025/actascibiolsci.v33i4.6134

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    Fúlvio Aurélio Morais Freire

    2011-09-01

    Full Text Available Este estudo avaliou o desempenho zootécnico do camarão marinho Litopenaeus vannamei em diferentes tipos de substratos não consolidados. Juvenis (0,97 ± 0,27 g foram aclimatados na densidade de 52 m-2, em 21 caixas de polietileno com biofiltros individuais, em um sistema fechado de filtração contínua, sendo submetidos aos tratamentos: 1 A = 25% silte + 25% argila + 25% areia muito fina + 25% areia fina; 2 B = 50% areia fina + 50% areia muito fina e 3 Controle = sem substrato, em um delineamento experimental completamente casualizado, com três tratamentos e sete repetições cada. Os animais foram alimentados com 12% da biomassa/dia com ração peletizada (35% de proteína bruta, ofertada em bandejas e parcelada em duas ofertas diárias (8 e 16h. O estudo teve duração de 48 dias, sendo avaliados: consumo aparente de ração, eficiência alimentar, taxa de crescimento específico, sobrevivência e ganho de peso. Foram acompanhados diariamente salinidade, pH, amônia e temperatura da água. A análise estatística dos dados se deu por meio dos testes Anova ou Kuskal-Wallis, em função da sua parametricidade. Não foram observadas diferenças significativas com relação aos fatores avaliados nas diferentes granulometrias de sedimento testadas, indicando que estas não exerceram influência sobre o desempenho zootécnico de L. vannamei juvenil.This study aimed to evaluate the zootechnical performance of Litopenaeus vannamei shrimp at different types of unconsolidated substrate. Juvenile shrimp (0.97 ± 0.27 g were acclimatized, in a stocking density of 52 m-2, to 21 polyethylene boxes (50 L connected individually to biofilters in a closed water exchange system. The animals were submitted to the treatments: 1 A = 25% silt + 25% clay + 25% very fine sand + 25% fine sand; 2 B = 50% fine sand + 50% very fine sand and 3 Control = no sediment, with seven replications each. Photoperiod was set on 12L: 12D. Shrimps were fed twice a day (at 08

  18. Culture of white shrimp (Litopenaeus vannamei Boone, 1931 with zero water exchange and no food addition: an eco-friendly approach Cultivo de camarón blanco (Litopenaeus vannamei Boone, 1931 sin recambio de agua y sin adición de alimento formulado: un sistema amigable con el ambiente

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    Juan Manuel Audelo-Naranjo

    2012-07-01

    Full Text Available Juveniles (3.5 ± 0.3 g of the white shrimp Litopenaeus vannamei were grown during 40 days with no water exchanges, no food addition and four initial densities (25, 50, 75 and 100 g m-3, corresponding to between 8 and 32 shrimp m-2, to determine growth rates, which could be achieved using the periphyton growing on artificial substrates as the only food source. The experimental culture units were 12 polyethylene 1 m³ cylindrical tanks with 4.8 m² of total submerged surface (bottom and walls, provided with 7.2 m² of artificial substrate (Aquamats™. There were no significant differences in the ammonia and nitrite concentrations determined in the four treatments (0.17-0.19 and 0.10-0.11 mg L-1, respectively, which remained below the respective levels of concern for shrimp cultures. Mean survival was similar, and ranged from close to 91 to 97%, whereas there were significant differences in mean individual weight, which ranged from 11.9-10.6 g shrimp-1 for the two low initial densities (25 y 50 g m-3, to 8.3-7.7 g shrimp-1 for the other treatments. However, because of the high survival and of the higher initial density, the best biomass yield was with 100 g m-3. The final nitrogen contents of sediment and water were lower than the initial values, and between 36 and 60% of the difference was converted into shrimp biomass.Durante 40 días se cultivaron juveniles de camarón blanco Litopenaeus vannamei con un peso individual de 3,5 ± 0,3 g y biomasas iniciales de 25, 50, 75 y 100 g m-3 (equivalente a 8-32 ind m-2, sin cambios de agua y adición de alimento, para determinar la tasa de crecimiento usando como única fuente de alimentación el perifiton desarrollado en sustratos artificiales. Se utilizaron estanques cilíndricos de polietileno de 1 m³ con tres réplicas por tratamiento, con una superficie de 4,8 m² (paredes y fondo y 7,1 m² de sustrato artificial (Aquamats™. No se encontraron diferencias significativas entre las concentraciones

  19. Un estudio de caso en el análisis de la distribución de frecuencias de tallas de Litopenaeus vannamei (Boone, 1931 mediante el uso de estimadores de densidad por Kernel A case study of length frequency distribution analysis of Litopenaeus vannamei (Boone, 1931 using kernel density estimators

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    Gustavo Rivera-Velázquez

    2010-01-01

    Full Text Available Se presenta el uso del estimador de densidad por Kernel (EDK, como una herramienta moderna para analizar la distribución de frecuencia de tallas de Litopenaeus vannamei en su etapa estuarina. Los datos fueron obtenidos en 22 sitios de muestreo dentro del sistema lagunar-estuarino Carretas-Pereyra, a intervalos mensuales entre marzo 2004 y agosto 2005 abarcando las dos épocas del año; estío y precipitación. El camarón fue capturado con atarraya (esparavel de cuatro metros de diámetro y malla de 10 mm. La distribución de cada muestra fue analizada mediante EDKs, usando la función ponderal gaussiana y la amplitud de banda bootstrap. Los valores de las modas dominantes en el tiempo fueron ajustados a la función de crecimiento de von Bertalanffy. Los resultados sugieren un reclutamiento continuo al área de pesca pero con pulso bimodal. La tasa media de crecimiento del camarón fue igual en ambas estaciones climáticas. Los parámetros estimados son similares a los registrados en investigaciones previas por otros autores para la especie en sistemas cercanos. El estudio muestra como el uso de los EDKs seguidos por un método de análisis del crecimiento (en este caso se empleo el análisis de la progresión modal, es un camino objetivo y preciso para el estudio de la distribución de frecuencias de tallas y su aplicación en la estimación de parámetros importantes en la dinámica poblacional de especies de importancia pesquera.This paper, introduces the use of kernel density estimators (EDK's as a modern tool for examining the length-frequency distribution of Litopenaeus vannamei in its estuarine stage. The data were obtained monthly at 22 sampling sites in the Carretas-Pereyra lagoon-estuarine system, from March 2004 to August 2005, covering both seasons of the year: dry and rainy. Shrimp were caught with a 4 m diameter cast net of 10 mm mesh size. The size distribution of each sample was analyzed by means of EDK's, using the Gaussian

  20. Physicochemical properties of cowpea (Vigna unguiculata L. Walp. meals and their apparent digestibility in white shrimp (Litopenaeus vannamei Boone Propiedades fisicoquímicas de harinas de frijol yorimón (Vigna unguiculata L. Walp. y su digestibilidad aparente en camarón Litopenaeus vannamei Boone

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    Martha Elisa Rivas-Vega

    2009-04-01

    Full Text Available The effect of different feed processing methods on the physicochemical properties, and apparent digestibility of cowpea (Vigna unguiculata meals as ingredients in diets for white shrimp (Litopenaeus vannamei was investigated. Five experimental cowpea meals were prepared: whole raw (WRC, dehulled (DC, cooked (CC, germinated (GC and extruded (EXC. The physicochemical properties of the meals were evaluated using differential scanning calorimetry. The meals were included at 15% in diets for L. vannamei (15.4 g to determine firmness of pellets and in vivo digestibility of nutrients by using chromic oxide as inert marker. Six diets were evaluated: a control diet, and five diets containing the different cowpea meals. Transition enthalpy significantly decreased after thermal treatment, from 6.1 J/g in WRC to 1.4 J/g in CC, and disappeared in EXC. Firmness of pellets varied from 1.1 N in the EXC diet to 2.8 N in the WRC diet. A significant negative correlation between transition enthalpy and carbohydrate digestibility was found. Dry matter, protein, carbohydrate and lipid digestibility of cowpea meals significantly increased after germinating, cooking or extruding. It is concluded that germinated, cooked and extruded cowpea meals are highly digestible for shrimp and that enthalpy of transition is negatively correlated with the digestibility of carbohydrates.Se evaluó el efecto de diferentes procesos sobre las propiedades fisicoquímicas y digestibilidad aparente de la harina de frijol yorimón (Vigna unguiculata como ingrediente en alimentos para camarón blanco (Litopenaeus vannamei. Se elaboraron cinco harinas de frijol yorimón: entero crudo (WRC, decorticado (DC, cocido (CC, germinado (GC y extruido (EXC. Las características térmicas de las harinas fueron evaluadas usando calorimetría diferencial de barrido. Se elaboraron seis alimentos experimentales: un alimento control y cinco alimentos conteniendo 15% de las diferentes harinas de frijol yorim

  1. Phytoplanktonic composition of three cultivation systems used in Litopenaeus vannamei (BOONE, 1931 marine shrimp farms = Composição fitoplanctônica em três sistemas de cultivo do camarão marinho Litopenaeus vannamei (BOONE, 1931

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    Michelle Pereira Melo

    2010-07-01

    Full Text Available The aim of this work is to assess the different compositions of phytoplankton in three cultivation systems of marine shrimps Litopenaeus vannamei (BOONE, 1931, denominated as organic, intensive and semi intensive. The samples were done fortnightly, when phytoplankton was collected by a net for phytoplankton, 65 ƒÊm mesh, being then filtrated in a total volume of water of 100 L, and preserved in formaldehyde solution at 4% and identified according to the methodology of Cordeiro et al. (1997. The results show that the densities of Diatoms were of 16.65, 10.47 and 7.57 cel. 103 mL-1 for the organic, intensive and semi intensive cultivations, respectively. As for cyanobacteria, the average figures were 42.06 cel. 103 mL-1 forsemi intensive 17.27 cel. 103 mL-1, in the intensive cultivation and 6.11 cel. 103 mL-1 for the organic cultivation system. The dinoflagellates had the highest cellular density in the phytoplankton community analyzed with 61.9 cel. 103 mL-1 in the intensive cultivation, 0.33 and 0.03 cel. 103 mL-1 for both semi intensive and organic cultivation systems respectively. Euglenas presented the results of 4.98 and 14.86 cel. 103 mL-1 only for semi intensive and intensive cultivations. It was then concluded that all cultivations presented average rates below recommended for such studied systems.Conduziu-se esse trabalho com o objetivo de avaliar as diferentes composicoes fitoplanctonicas em tres sistemas de cultivo para o camarao marinho Litopenaeus vannamei (BOONE, 1931, denominados de organico, intensivo e semiintensivo. As amostragens foram realizadas quinzenalmente, onde o fitoplancton foi coletado atraves de uma rede de plancton, com malha de 65 ƒÊm, sendo filtrado um volume de agua total de 100 litros, que foram preservadas em solucao de formol a 4% e identificadas segundo a metodologia de Cordeiro et al. (1997. Os resultados mostram que as densidades de diatomaceas foram de 16,65; 10,47 e 7,57 cel. 103 mL-1, respectivamente

  2. ISOLATION AND IDENTIFICATION OF STRAINS OF Salmonella spp IN RED-FOOT-TORTOISES FROM WILDLIFE TRADE ISOLAMENTO E IDENTIFICAÇÃO DE CEPAS DE Salmonella spp DE JABUTIS-PIRANGA ORIUNDOS DO TRÁFICO DE ANIMAIS SILVESTRES

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    Oberdan Coutinho Nunes

    2010-04-01

    Full Text Available We investigated Salmonella sp. in red-foot tortoise (Chelonoides carbonaria because of the fact this particular reptile shows high frequency of illegal commerce, and also because this situation represents a serious risk for health. We collected feces of 89 turtles from the wildlife center “Chico Mendes” - IBAMA/SUPES-BA. Characteristic colonies of Salmonella sp. were isolated in 12.36% of the tortoises (11/89. Nine colonies were identificated at the Adolfo Lutz Institute, distributed into six different sorotypes: S. enterica salamae 47:b:-; S. enterica houtenae 21:g,z51; S. Panama; S. Poona; S. Javiana e S. Michigan. All of them, according to literature, without previous description of infection in C. carbonaria whatsoever. However, there are previous cases of infections and antibiotics resistance involving these sorotypes, confirming the risk represented by wildlife trade to public health concerning the children’s exposition to the source of infection as well as the dissemination of resistant sorotypes.

    KEY WORDS: Epidemiology, reptiles, zoonosis.

    Este trabalho objetivou investigar a ocorrência de Salmonella sp. em jabutis-piranga (Chelonoides carbonaria, dada sua elevada frequência no comércio ilegal e risco que representa para a saúde. Coletaram-se fezes de 89 jabutis mantidos no Centro de Triagem de Animais Silvestres Chico Mendes – IBAMA/SUPES-BA. Isolaram-se colônias características de Salmonella sp. em 12,36% dos jabutis (11/89. Destas, nove foram identificadas no Instituto Adolfo Lutz (São Paulo, distribuindo-se em seis diferentes sorovares: S. enterica salamae 47:b:-; S. enterica houtenae 21:g,z51; S. Panama; S. Poona; S. Javiana e S. Michigan, todas, segundo a literatura consultada, sem prévia descrição para C. carbonária. No entanto, estão presentes em relatos de infecções e em estudos sobre resistência a antibióticos, confirmando o risco representado pelo tráfico de animais para a sa

  3. Animales silvestres como reservorios de leptospirosis en Chile: Una revisión de los estudios efectuados en el país Wild animals as reservoirs of leptospirosis in Chile.: Revision of studies in the country

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    J. ZAMORA

    1999-01-01

    Full Text Available Se entrega información sobre ratas y ratones capturados en laprovincia de Valdivia que actúan como reservorios de serovares deLeptospirainterrogans y Leptospira borgpeterseni, así como tambiénse dan a conocer resultados de otras especies animales capturados en lazona central del país. Se analizan los porcentajes de positividaddetectados por especie animal y el consecuente riesgo que significan estosreservorios animales para los animales domésticos y para el hombre. Se completa la información sobre los huéspedes de mantenimientode esta espiroquetosis en animales de vida libre, de acuerdo a una revisiónde la literatura especializada del exterior sobre el tema. Todo ello, enun intento de entregar documentación que alerte sobre los eventualesportadores existentes en nuestro medio y, por ende, ser una posible fuentede infección de enfermedades transmisibles a la especie humana ya diferentes especies de animales domésticos, Por último, se concluye que es del todo conveniente y necesarioque las autoridades correspondientes se preocupen de incentivar y fomentarestudios no sólo sobre leptospirosis, sino que también deotras enfermedades infecciosas y del estado sanitario en general de lafauna silvestre del país, financiando y licitando proyectos de investigación.La información que se logre de los trabajos que se realicen seráde considerable valor en la conservación del equilibrio ecológico,en medicina veterinaria preventiva y en salud públicaInformation is given regarding rats and mice captured in the provinceof Valdivia as reservoirs of serovars of Leptospira interrogansand Leptospira borgpeterseni; results obtained from other wild animalscaptured in the central area of the country are also included. The percentagesof positiveness detected by animal species as well as the consequent riskof these reservoirs for domestic animals and humans are analysed. Additional information on the subject in relation to reservoirs of leptospirasin

  4. Capítulo V: evaluación de especies silvestres de Lycopersicon sp. como fuente de resistencia al insecto plaga Scrobipalpula absoluta (Meyrick y su intento de transferencia a Lycopersicon esculentum Mill

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    Vallejo Cabrera Franco Alirio

    1994-12-01

    Full Text Available

    En condiciones de campo, se evaluaron 32 introducciones silvestres de Lycopersicon sp. y una de la especie cultivada, L. esculentum Mill, con el fin de determinar la resistencia a Scrobipalpula absoluta (Meyrick. Se utilizó el diseño experimental bloques completos al azar con 4 repeticiones. Como bordes se colocaron tres variedades comerciales susceptibles al insecto (Chonto "Mata Verde", Napoli Y Chonto "Santa Cruz Kada" con el fin de tener una fuente de infestación natural y permanente del cogollero dentro del experimento. Se evaluaron los caracteres número de cogollos afectados, número de hojas dañadas, número de ampollas e intensidad de daño. Todas las introducciones de L. hirsutum y L. peruvianum fueron altamente resistentes. Las introducciones 1406 y 1407 de L. pimpinellifolium también fueron altamente resistentes. La introducción 1759 de L. esculentum y L. esculentum var. ceraciforme fueron muy susceptibles. Las introducciones de L. pimpinellifolium y L. hirsutum no tuvieron dificultad para hibridarse con L. esculentum var. Tropic. El cruzamiento L. esculentum x L. peruvianum no presentó semilla híbrida y se requirió efectuar cultivo de embriones inmaduros, in vitro, utilizando el medio Murashige & Skoog pero sin lograrse resultados positivos.

    Thirty two (32 wild accessions of Lycopersicon sp. and one (1 cultivated accession of Lycopersicon esculentum Mill were evaluated to determinate the resistance to Scrobipalpula absoluta (Meyrick. The experiment was conducted with a complete random design and four repetitions. Three (3 susceptibles cultivated accessions of L. esculentum (Chonto "Mata Verde", Napoli and Chonto "Santa Cruz Kada" were sowed to have natural infestation of S. absoluta. Damage intensity, number of buds damaged, number of leaves demaged and number of "blister" in the leaves were evaluated, All evaluated accessions of L. peruvianum showed heigh resistence. 1406 and 1407 accessions of L. pimpinellifolium

  5. Teste de ELISA indireto para diagnóstico sorológico de leishmaniose visceral em canídeos silvestres Indirect ELISA for the serological diagnosis of visceral leishmaniasis in wild canids

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    Paulo R.B. Ferreira

    2013-04-01

    Full Text Available Na América do Sul, alguns canídeos silvestres são considerados reservatórios naturais da Leishmania chagasi. A resposta imunológica desses animais à Leishmania é pouco conhecida, havendo a necessidade de métodos diagnósticos adequados para esse fim. No presente estudo, é descrita a padronização do ensaio imunoenzimático indireto (ELISA para o diagnóstico sorológico de leishmaniose visceral em canídeos silvestres brasileiros. Foram estudadas amostras de soro e plasma de 12 canídeos cativos: sete lobos-guará (Chrysocyon brachyurus, três raposinhas (Lycalopex vetulus e dois cachorros-do-mato (Cerdocyon thous. As amostras de um C. brachyurus e uma L. vetulus, cativos em área endêmica para LV, que apresentavam doença clínica e positividade em testes de Imunofluorescência Indireta e Reação em Cadeia de Polimerase, foram utilizadas como controles positivos. Foram comparados os conjugados anti-IgG de cão e proteína A, ambos ligados a peroxidase, cujos testes detectaram quatro (04/12 e três (03/12 C. brachyurus soropositivos para anticorpos anti-Leishmania sp., respectivamente. As médias das densidades ópticas (DOs das amostras negativas foram nitidamente mais baixas do que as médias das DOs dos positivos tanto no ELISA com anti-IgG de cão (4,8 vezes como com proteína A (15,5 vezes. Os soros de três C. brachyurus positivos no ELISA indireto foram avaliados por Western blotting e identificaram 22 bandas, sendo imunodominantes as de peso molecular de 19, 22, 24, 45 e 66 kDa. Os testes ELISA com a proteína A e o conjugado anti-IgG de cão apresentaram respectivamente concordância excelente (Kappa = 1; pIn South America, some wild canids are considered natural reservoirs of Leishmania chagasi. The immunological response of wild canids to Leishmania is not well understood, and the development of diagnostic methods is necessary for such purpose. In the present study, the standardization of an enzyme-linked immunosorbent

  6. Identificación, establecimiento in vitro y análisis fitoquímico preliminar de especies silvestres de ñame (Dioscorea spp. empleadas con fines medicinales

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    Víctor Andrés Ramos Duarte

    2015-05-01

    Full Text Available Título en ingles: Identification, in vitro establishment and preliminary phytochemical analysis of wild yam (Dioscorea spp. used for medicinal purposesTítulo corto: Identificación, establecimiento in vitro y análisis fitoquímico preliminar de especies silvestres de ñameResumen:  Tubérculos del género Dioscorea comercializados con fines medicinales, fueron recolectados con el propósito de lograr su establecimiento a condiciones in vitro. Previamente se lograron identificar taxonómicamente las especies y por medio de análisis fitoquímicos demostrar su potencial farmacéutico. El material recolectado fue identificado como Dioscorea coriacea, D. lehmannii, D. meridensis, D. polygonoides y una especie comestible D. trifida. Tubérculos recolectados de centros de acopio y traídos de campo fueron lavados, desinfectados, asperjados con Ácido Giberélico (AG3 y sembrados en sustrato BM-2®, en invernadero a 18°C día y 10°C noche. Los tubérculos completos o por secciones fueron almacenados en bolsas herméticas a temperatura ambiente. Posteriormente se desinfectó material vegetal de las especies D. coriacea, D. lehmannii, D. meridensis y D polygonoides, seleccionando explantes de brotes sanos (D. coriacea / laboratorio para su establecimiento. Se evaluaron tres medios de cultivo para establecimiento, el que presentó los mejores resultados fue Medio Murashige & Skoog (1962 suplementado con BAP 1 mL/L, AG3 1 mL/L y Putrescina 2 mL/L. Para la extracción y análisis de metabolitos secundarios se utilizaron tubérculos de D. coriacea, D. lehmannii y D. polygonoides, empleando como solvente de extracción metanol. Se  encontró mayor concentración de extracto vegetal en D. coriacea (54%, y mediante cromatografía en capa delgada (CCD, se confirmó la presencia de saponinas, que resultó mayor en comparación con D. polygonoides especie reconocida por su alto contenido de saponinas. Estos resultados permitirán realizar análisis m

  7. 凡纳滨对虾幼虾的缬氨酸需要量%Valine Requirement of Juvenile Pacific White Shrimp, Litopenaeus vanname

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    周歧存; 王用黎; 黄文文; 霍雅文; 王猛强

    2015-01-01

    An 8-week feeding trial was conducted to study the effect of dietary valine content on growth per-formance, common nutrient composition and amino acid metabolic enzyme activities of juvenile Pacific white shrimp ( Litopenaeus vanname ) , and in order to determine the valine requirementof juvenile Pacific white shrimp. Six isonitrogenous and isoenergetic experimental diets ( crude protein content was about 40.0%, and crude lipid content was about 7. 5%) were formulated with fish meal and soybean meal as main protein sources,and the measued values of valine content ( dry matter basis) in those diets were 1. 56%, 1.64%, 1.72%, 1.82%, 1.90% and 1.96%, respectively. A total of 540 juvenile Pacific white shrimp with the body weight was about 0.3 g were randomly divided into 6 groups with 3 replicates per group ( diet) and 30 juven-iles per replicate. The results showed that the weight gain rate, specific growth rate, protein retention rate and feed efficiency were firstly increased and then decreased with dietary valine content increasing, and the biggest values of them were found when dietary valine content was 1.82%. When dietary valine content was 1.72%, the crude protein content of whole body had the highest value, and it was decreased in different degree with di-etary valine content further increasing. The crude protein content of muscle had the highest value when dietary valine content was 1. 96%, and it was significantly higher than when dietary valine content was 1. 56% or 1.72% ( P0.05) . The highest values of alanine aminotransferase activ-ity in serum and hepatopancreas were all found in the group with the highest content of valine ( the group with the valine content was 1.96%) , and the highest values of alanine aminotransferase activity in muscle and alka-line phosphatase activity in hepatopancreas were all found in the group with the valine content was 1.90%. Di-etary valine content had no significant effect on aspartate aminotransferase activity in

  8. 超高压结合酶法消减南美白对虾虾肉中的过敏原%Allergen Elimination from Litopenaeus vannamei Meat by Ultra-high Pressure Treatment Coupled with Papain Hydrolysis

    Institute of Scientific and Technical Information of China (English)

    谢丹丹; 胡志和; 薛璐; 张博洋

    2012-01-01

    The aim of this study was to investigate the synergistic effect of ultra-high pressure treatment and papain hydrolysis on allergen elimination from the meat of Litopenaeus vannamei. Fresh Litopenaeus vannamei was homogenized after removing the head, tail, shell, and catgut. Three methods, ultra-high pressure treatment, ultra-high pressure treatment followed by papain hydrolysis and simultaneous ultra-high pressure treatment and papain hydrolysis were used to eliminate allergens from the Litopenaeus vannamei homogenate. Indirect ELISA was used to evaluate the results of allergen elimination and the optimal conditions for allergen elimination were explored. The results showed that the optimal conditions for allergen elimination by ultrahigh pressure treatment were 200 MPa, 35 min and 40 ℃. Under these conditions, the optical density at 492 nm (OD492m) after the reaction between antibody and treated Litopenaeus vannamei homogenate was 0.1986. The optimal conditions for ultra-high pressure treatment followed by papain hydrolysis were substrate concentration of 10 g/mL, hydrolysis temperature of 60℃ and enzyme-to-substrate ratio of 1:200, resulting in an OD492nm of 0.0487. The optimal conditions for simultaneous ultra-high pressure treatment in the presence of papain were 300 MPa, 35 min and 40 ℃, which revealed an OD492nm of 0.0516. Based on the above studies, ultra-high pressure treatment is effective for allergen elimination from Litopenaeus vannamei, but inferior to ultra-high pressure treatment followed by papain hydrolysis and ultra-high pressure treatment in the presence of papain.%以虾肉为原料,研究超高压对酶法消减南美白对虾虾肉过敏原的强化作用。将南美白对虾去头、去尾、去壳、去肠线后用匀浆机匀浆,制成虾肉酱,分别采用超高压法、超高压处理后再用木瓜蛋白酶水解、超高压条件下直接酶解的方法消减其过敏原,用间接酶联免疫吸附法检测过敏原消减

  9. Diversidade de helmintos intestinais em mamíferos silvestres e domésticos na Caatinga do Parque Nacional Serra da Capivara, Sudeste do Piauí, Brasil Diversity of wild and domestic mammal's intestinal helminths from the Caatinga of the Parque Nacional Serra da Capivara, Southeast of Piauí, Brazil

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    Brandão Martha Lima

    2009-12-01

    Full Text Available Estudos sobre biodiversidade permitem avaliações ecossistêmicas e monitoramentos de impactos e mudanças ambientais. A diversidade parasitária pode refletir o processo coevolutivo entre parasitos e hospedeiros, e mudanças ambientais que permitem a perda, ganho ou a manutenção das espécies nesse processo. O presente trabalho utilizou espécies/morfotipos de ovos de helmintos encontrados em fezes de sete mamíferos silvestres (os grupos Dasypodidae e grandes felinos, e as espécies: Tamandua tetradactyla, Cebus apella, Alouatta caraya, Cerdocyon thous, Pecari tajacu e dois domésticos (Canis familiaris e Sus scrofa, que cohabitam o Parque Nacional Serra da Capivara (PNSC e seu entorno, para analisar a diversidade dos helmintos intestinais desses mamíferos. Neste trabalho, utilizou-se achados da fauna helmintológica de mamíferos silvestres e domésticos, para avaliar um possível fluxo de helmintos entre estes dois grupos hospedeiros usando Unweighted Pair Group Method with Arithmetic Mean (UPGMA dos hospedeiros com base na composição de suas faunas helmintológicas. Os resultados indicam que a região do PNSC, ainda mantém condições ambientais que ainda propiciam a manutenção da composição da fauna de helmintos de mamíferos silvestres, diferente da encontrada nos animais domésticos.Biodiversity studies allow ecosystem assessment and monitoring of environmental changes and impacts. Parasite diversity could reflect the host/ parasite coevolutionary process and the environment changes that permit the loss, gain or maintenance of species. This survey used species/morphotypes of helminths eggs found in feces from seven wild mammal species (the groups Dasypodidae and Large Cats, and Tamandua tetradactyla, Cebus apella, Alouatta caraya, Cerdocyon thous, Pecari tajacu and from two domestic species (Canis familiaris and Sus scrofa, which occur within the Serra da Capivara National Park (PNSC and surrounding areas in order to analise

  10. Entomological investigation of a sylvatic yellow fever area in São Paulo State, Brazil Investigação entomológica em área de ocorrência de febre amarela silvestre no Estado de São Paulo, Brasil

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    Vera L. F. de Camargo-Neves

    2005-08-01

    Full Text Available Following reports of two autochthonous cases of sylvatic yellow fever in the State of São Paulo, Brazil, in 2000, entomological surveys were conducted with the objective of verifying the occurrence of vector species in forest environments close to or associated with riparian areas located in the western and northwestern regions of the State. Culicidae were captured in 39 sites distributed in four regions. Haemagogus leucocelaenus and Aedes albopictus were the most abundant species and were captured in all the regions studied. H. leucocelaenus was the most abundant species in the municipalities of Santa Albertina and Ouroeste, where the two cases of sylvatic yellow fever had been reported. Mosquitoes from the janthinomys/capricornii group were only found at eight sites in the São José do Rio Preto region, while Sabethes chloropterus was found at one site in Ribeirão Preto. H. leucocelaenus showed its capacity to adapt to a secondary and degraded environment. Our results indicate a wide receptive area for yellow fever transmission in the State of São Paulo, with particular emphasis on the possibility of H. leucocelaenus being involved in the maintenance of this sylvatic focus of the disease.O registro de dois casos autóctones de febre amarela silvestre no Estado de São Paulo, Brasil, em 2000, desencadeou investigações entomológicas com o objetivo de verificar a ocorrência das espécies vetoras em ambientes florestais próximos ou associados às zonas ribeirinhas, situados nas regiões oeste e noroeste do Estado. As capturas foram realizadas em 39 localidades distribuídas por quatro regiões do Estado. Haemagogus leucocelaenus e Aedes albopictus foram as espécies mais abundantes e capturadas em todas as regiões. H. leucocelaenus foi a espécie mais abundante nos municípios de Santa Albertina e Ouroeste, onde os casos de febre amarela silvestre foram registrados. Mosquitos do grupo janthinomys/capricornii foram encontrados em oito

  11. 不同贮藏温度对凡纳滨对虾理化性质的影响%Effects of different storage temperature on the physical and chemical properties of Litopenaeus vannamei

    Institute of Scientific and Technical Information of China (English)

    张强; 代文婷; 金新文

    2016-01-01

    Litopenaeus vannamei were stored at -5 ℃,-1 8 ℃,-35 ℃ and -60 ℃ respectively,the changes of shrimp freshness,thawing loss rate and textural properties were analyzed to study the effects of differ-ent storage temperature on the physical and chemical properties of Litopenaeus vannamei.The thawing loss rate of shrimp was low,and the texture property was better when stored at -5 ℃,-35 ℃ and -60 ℃,but the shelf-life was short when stored at -5 ℃,and it became inedible when stored for 30 days.When stored at -1 8 ℃, the freshness was good,but after thawing,the juice loss was serious,and the texture property of shrimp decreased obviously.When stored at -35 ℃ and -60 ℃,the shrimp quality was not much difference,the shrimp freshness could be kept for a long time.The equipment and operation requirements are very high,and the energy consump-tion is large when stored at -60 ℃.Therefore,-35 ℃ was the ideal storage temperature for Litopenaeus van-namei.%将凡纳滨对虾分别在-5℃、-18℃、-35℃、-60℃进行贮藏,分析虾肉新鲜度、解冻损失率、质构性能的变化,研究不同贮藏温度对凡纳滨对虾理化性质的影响。结果表明:在-5℃、-35℃、-60℃下贮藏,虾肉解冻损失率低,质构性能保持较好,但-5℃保鲜期短,30 d 已不可食用。在-18℃下冷冻贮藏,新鲜度保持良好,但解冻后汁液流失严重,虾的质构性能下降较明显。在-35℃与-60℃下贮藏,虾肉品质差异不大,均能够长时间保持虾新鲜度。由于在-60℃下贮藏对设备和操作要求都很高,且能耗大,因此凡纳滨对虾在-35℃下贮藏较为理想。

  12. The ecology of Triatoma sordida in natural environments in two different regions of the state of Minas Gerais, Brazil Ecologia do Triatoma sordida no ambiente silvestre de duas diferentes regiões do estado de Minas Gerais, Brasil

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    L. Diotaiuti

    1993-06-01

    Full Text Available A study was undertaken about T. sordida in the natural environment in two different regions of the state of Minas Gerais: Itapagipe (Triângulo, an area of cerrado modified by the formation of fields of pasture and agriculture, and Mato Verde (north an area of transition between caatinga and cerrado with profound deforestation in the last years due to the expansion of cotton cultivation. In both regions the principal ecotopes identified were hollow trees and the bark of live or dead trees, where the occurrence of a food source is not frequent. In this environment, the triatomines utilize various food sources; opposums appear to represent an important source of infection. In the north of Minas, a greater concentration of reservoirs and vectors was observed than in the Triángulo which could explain the higher level of infection of the triatomines in the north. Close attention to the process of domiciliation of T. sordida in the north of Minas is recommended where an extensive intervention by man in the natural environment has occurred and where a rise in the population of triatomines in the peridomestic environment has been observed in recent years.Foi realizado um estudo sobre o T. sordida em ambiente silvestre em duas diferentes regiões do estado de Minas Gerais: Itapagipe (Triângulo, área de cerrado modificado para formação de campos de pastagem e agricultura, e Mato Verde (norte uma área de transição entre a caatinga e o cerrado, profundamente desmatada nos últimos anos com a finalidade de expansão do cultivo de algodão. Em ambas as regiões, os principais ecotopos identificados corresponderam a buracos e cascas de árvores vivas ou mortas, onde a ocorrência de fontes de alimentação não é frequente. Neste ambiente, os triatomíneos utilizam-se de variadas fontes de alimentação; gambás parecem representar urna importante fonte de infecção. No norte de Minas, observou-se urna maior concentração de reservatórios e vetores

  13. Distribución geográfica de las especies cultivadas de Amaranthus y de sus parientes silvestres en México Geographical distribution of cultivated species of Amaranthus and their wild relatives in Mexico

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    Eduardo Espitia-Rangel

    Full Text Available La presente investigación tuvo como objetivo obtener los patrones de distribución de Amaranthus cruentus, Amaranthus hypochondriacus y sus parientes silvestres, mediante el uso de Sistemas de Información Geográfica, además de realizar un patrón de variación geográfica de las zonas donde se localizan. El género Amaranthus está distribuido a lo largo de México. A. cruentus y A. hypochondriacus se distribuyen en la parte centro sur del país, mientras que A. powellii está distribuido del centro al norte de México. Por otro lado, A. hybridus se puede cultivar prácticamente en todo México, por lo que fue la especie que presentó la mayor adaptación en comparación a las otras tres especies que mostraron una distribución más limitada. De acuerdo con la hipótesis que A. hybridus puede ser el ancestro más probable de A. cruentus y según los resultados obtenidos en este estudio, esta hipótesis podría ser cierta debido que en toda la distribución de A. cruentus está presente A. hybridus. Cabe mencionar, sobre la hipótesis que A. hypochondriacus puede ser un híbrido entre A. cruentus y A. powellii, se puede concluir que es cierta ya que A. hypochondriacus presentó un patrón de variación latitudinal parecido a A. powellii y un patrón de variación altitudinal similar a A. cruentus; además que todas ellas convergen en el Valle de México.The aim of this work was to obtain the distribution patterns of Amaranthus cruentus, Amaranthus hypochondriacus and its wild relatives using Gorgraphic Information Systems, as well as creating a geographic variation pattern of the areas in which they are found. The genus Amaranthus is distributed throughout Mexico. A. cruentus and A. hypochondriacus are distributed in the south-central area of the country, whereas A. powellii is found in the center and north of Mexico. On the other hand, A. hybridus can be cultivated in practically any part of the country, making it the species with the

  14. Behavioral experiments in a wild passerine cause short-term reductions in parental provisioning and nestling mass Experimentos conductuales en un ave paserina silvestre causan reducciones de corto plazo en el cuidado parental y peso de pichones

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    WOUTER F.D VAN DONGEN

    2009-06-01

    Full Text Available Research on wild animals often involves the capture and temporary removal of individuals from their territory. Although the acute effects of such procedures are well understood in terms of stress hormone profiles, their effect on the behaviour of captured individuals after release is poorly known. Additionally, for socially-monogamous individuals captured whilst breeding, little is known regarding whether the remaining individual compensates for the temporary loss of its partner and whether offspring condition or survival ultimately decreases due to any reductions in parental provisioning. We investigated the influence of field-based experiments requiring temporary adult removal on adult provisioning behaviour and nestling mass in the thorn-tailed rayadito (Aphrastura spinicauda, a Chilean furnariid species with equal parental effort between sexes. During the absence of the removed individual, remaining individuals did not increase nestling provisioning rates while, upon their release, removed individuals did not return to nestling feeding for at least 38 minutes and typically much longer. This drastic reduction in combined parental provisioning negatively affected nestling mass, while average nestling mass increased during a control period, nestlings failed to gain weight during and subsequent to parental removal. Finally, our experiments did not affect fledging probability. Overall, our results indicate that the temporary removal of rayaditos from their territories can cause short-term reductions in parental provisioning and nestling weight, but is unlikely to lead to any long-term effects on nestling survival.Las investigaciones en animales silvestres frecuentemente requieren la captura y remoción de individuos de sus territorios. Si bien se conocen los efectos a nivel hormonal de tales métodos, sus efectos sobre la conducta de los individuos después de la liberación son escasamente conocidos. Además, para individuos de especies mon

  15. Caracterización de micorrizas establecidas entre dos hongos comestibles silvestres y pinos nativos de México Characterization of mycorrhizas established between two wild edible mushrooms and native pines of Mexico

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    Violeta Carrasco-Hernández

    Full Text Available Las especies forestales dependen de la simbiosis que realizan con los hongos ectomicorrízicos, para un crecimiento óptimo. Adicionalmente México es un importante reservorio cultural y biológico de especies de hongos silvestres comestibles a nivel mundial. A pesar de la gran importancia ecológica, antropológica y práctica, existen escasas descripciones de las ectomicorrizas formadas entre hongos ectomicorrizicos comestibles y plantas nativas de México. En el presente trabajo, se realizó la descripción morfológica de las ectomicorrizas de los hongos comestibles Laccaria bicolor y L. proxima en asociación con Pinus patula y P. pseudostrobus. La síntesis de las micorrizas estudiadas se efectuó mediante la inoculación con esporas en un sustrato estéril, bajo condiciones de invernadero. Se describen las ectomicorrizas formadas en plantas 397 días después de la siembra de pinos y de la inoculación con las especies fúngicas. Se observaron diferencias en la micromorfología de las ectomicorrizas de ambas especies fúngicas, siendo los principales caracteres distintivos, la longitud de la ectomicorriza, el tipo de ramificación, la forma de las puntas no ramificadas y el color de los ápices. Ambas especies presentaron el mismo arreglo anatómico de la capa externa del manto. Se adiciona también la descripción de las esporomas de Laccaria bicolor y L. proxima. Este estudio se realizó en el laboratorio de micorrizas del Programa de Edafología del Colegio de Postgraduados en Ciencias Agrícolas (CP en 2009.For an optimum growth, forest species depend on the symbiosis with ectomycorrhizal mushrooms. Additionally, Mexico is an important cultural and biological reserve of species of wild edible mushrooms at world level. In spite of huge ecological, anthropological and practical importance, there are scarce descriptions of ectomycorrhizas formed between edible ectomycorrhizal mushrooms and native plants of Mexico. In this work was done

  16. Experimental infection with different bacterial strains in larvae and juvenile Litopenaeus vannamei reared in Santa Catarina State, Brazil = Infecção experimental em larvas e juvenis de Litopaenaeus vannamei cultivados no Estado de Santa Catarina, Brasil

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    Celso Carlos Buglione

    2010-07-01

    Full Text Available This study evaluated the pathogenic characteristics of bacteria isolated from Litopenaeus vannamei during an outbreak at the Laboratory of Marine Shrimp, UFSC, Santa Catarina State, Brazil. Their virulence potential in larvae and juvenile shrimp and theeffects on the total haemocyte count, phenoloxidase activity and serum agglutinate titre were examined after experimental infection. Bacterial strains were isolated from larvae and adult shrimps, identified by the AP120E biochemical system as: two strains of Vibrioalginolyticus, three of Aeromonas salmonicida and one of Pasteurella multocida sp. and Pasteurella sp. All the bacterial strains isolated in this study caused mortality in shrimp. One strain of V. alginolyticus was responsible for 97.3 and 88.7% mortality in larvae and juvenil shrimps, respectively. The shrimp immunological system was influenced by experimental infection with V. alginolyticus. Decrease in the total haemocyte count and increase in the phenoloxidase activity and the serum agglutinate titre (p Este estudo avaliou as características patogênicas de cepas de bactérias isoladas de Litopenaeus vannamei durante surto de mortalidade no Laboratório de Camarões Marinhos, UFSC, Estado de Santa Catarina, Brasil. Seu potencial de virulência em larvas e juvenis de camarão marinho e os efeitos sobre a contagem total de hemócito, atividade de fenoloxidase e título aglutinante do soro foramavaliados após infecção experimental. As cepas bacterianas foram isoladas de larvas e de camarões adultos e identificadas bioquimicamente pelo sistema API20E como: duas cepas de Vibrio alginolyticus, três de Aeromonas salmonicida e uma de Pasteurella sp. e P. multocida. Todas as cepas isoladas provocaram mortalidade em L. vannamei, e uma de V. alginolyticus resultou em mortalidade de 97,3 e 88,7% para larvas e juvenis de camarões, respectivamente. O sistema imunológico dos camarões juvenis sofreu influência da infecção experimental

  17. El veterinario forense. Caza clandestina en fauna silvestre. Análisis forense y situacional de dos casos en el municipio de Tandil, Argentina (The Forensic Veterinary. Clandestine hunt in wild fauna. Forensic and situational studies in two cases. Tandil county. Argentina

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    Schettino, D.M.

    2007-04-01

    Full Text Available ResumenDos casos de caza clandestina de fauna silvestre ocurren con diferencia de meses en el municipio de Tandil, en los años2005 y 2006. Ambos afectaron a ciervos dama (Dama dama que fueron cazados en diferentes circunstancias. La utilización de perros entrenados para matar como también el uso de armas de guerra en áreas suburbanas está tácitamente prohibida en la legislación actual. Ambos casos son peritados en la FCV-UNCPBA y se analizan las condiciones de cada situación, el respaldo legal y los resultados obtenidos en amboscasos.

  18. Malófagos (Phthiraptera recolhidos de aves silvestres no Zoológico de São Paulo, SP, Brasil Chewing lice (Phthiraptera from wild birds in the São Paulo Zoo, State of São Paulo, SP, Brazil

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    Michel P. Valim

    2005-12-01

    Full Text Available Vinte e oito espécies de malófagos foram recolhidas de aves silvestres no acervo do Zoológico de São Paulo durante o ano de 2003. Vinte e três foram identificadas ao nível de espécie e cinco ao nível genérico. Os relatos de novas espécies no Brasil e novas associações parasitárias para a literatura são discutidos.Twenty-eight species of chewing lice were recovered from wild birds in the São Paulo Zoo during 2003. Twenty-three were identified to species level and five to the generic level. New species records for Brazil and new host-lice associations are discussed.

  19. Enfermedades infecciosas en camaronesPenaeus y langosta Panulirus. Situación actual.

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    Manuel Rubio Limonta

    2012-07-01

    Full Text Available ResumenEl conocimiento de la situación epidemiológica internacional de las enfermedades infecciosas que afectan la salud del camarón y la langosta tienen una importancia relevante, ignorar su significado ha facilitado el desarrollo de epidemias que han devastado áreas de cultivo en todo el mundo.AbstractThe lack of information on international epidemiological situation of the diseases that affect the health of shrimp and lobster have played an important role in the epidemics that have devastated cultivated areas worldwide.

  20. Evaluation of the pharmacological treatment of arterial hypertension associated to heart failure in Camarones town.

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    Pedro Miguel Milián Vázquez.

    2006-12-01

    Full Text Available Background: Arterial hypertension is a risk factor for many cardiovascular and cerebrovascular diseases. Objective: To evaluate the pharmacological treatment in patients with arterial hypertension, also suffering from heart failure. Methods: A descriptive-prospective study was carried out, this consisted in the use of prescription-indication drugs through a simple random sample study of 43 patients, representing the 35.2 % in six Family Clinical Units of the urban area of Camarones’ Communitarian Policlinic, Palmira, Cienfuegos, during the first semester of 2004. Results: the 51.2 % of the patients were included in the class II of the New York Heart Asociation’s classification, and the 55.8% were considered hypertense class II. The hypertensive drugs more used were the captopril and the clortalidone, and among the drugs associated to the hypertensive ones it was included the isosorbide dinitrate, the digoxin and the acetylsalicylic acid. The 87.3 % of the patients received a correct dose, and in the 88.9% it was followed an adequate administration interval. The prescription was considered adequate in the 65.1 % of the studied patients. Conclusions: the advances in the treatment of these diseases are due to different factors, even though the study shows that the treatment of the patient of the series is adecuate, it should be bettered as long as possible.

  1. Influência do aquecimento sobre diferentes métodos de titulação de SO2 residual em camarões Litopenaeus vannamei (Boone, 1931 - DOI: 10.4025/actascianimsci.v30i1.3437 Influence of heating under different titration methods of residual SO2 on shrimps Litopenaeus vannamei (Boone, 1931 - DOI: 10.4025/actascianimsci.v30i1.3437

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    Alfredo Oliveira Galvez

    2008-06-01

    Full Text Available O metabissulfito de sódio é o conservante mais aplicado na carcinicultura, com uso amparado em legislações vigentes no país, todavia, seu resíduo, dióxido de enxofre (SO2, em concentrações altas, pode provocar reações adversas à saúde. Este estudo teve como finalidade observar a influência do aquecimento sob diferentes métodos de titulação de SO2 residual em camarões Litopenaeus vannamei. Os camarões foram submetidos a nove concentrações de metabissulfito de sódio e avaliados diferentes métodos de detecção de SO2. O método da titulação Adolfo Lutz adaptado apresentou grande sensibilidade para detecção de SO2 residual. O método da titulação iodométrica com aquecimento possibilitou a detecção de maiores níveis de SO2, quando comparado ao método sem o aquecimento. O aquecimento interferiu negativamente no método da fita reativa. Conclui-se que o aquecimento influenciou positivamente a titulação iodométrica, não ocorrendo o mesmo com a fita reativa.Sodium metabisulfite is the most applied preservative in shrimp culture, with its use supported by current legislation; however, its residue, sulfur dioxide (SO2, in high concentrations can provoke adverse health reactions. This study evaluated the influence of heating under different methods of residual SO2 titration in em>Litopenaeus vannamei. Shrimps were treated with nine concentrations of sodium metabisulfite and evaluated by different methods of SO2 titration. The adapted Adolfo Lutz titration method presented the highest sensitivity for the detection of SO2. The method of iodometric titration with heating made possible the detention of higher levels of SO2, when compared to the same method without heating. The heating influenced negatively on the reactive ribbon method. It is concluded that the heating influenced positively on the iodometric titration, as opposed to the reactive ribbon.

  2. The impact of oxygen consumption by the shrimp Litopenaeus vannamei according to body weight, temperature, salinity and stocking density on pond aeration: a simulation - doi: 10.4025/actascibiolsci.v33i2.7018 The impact of oxygen consumption by the shrimp Litopenaeus vannamei according to body weight, temperature, salinity and stocking density on pond aeration: a simulation - doi: 10.4025/actascibiolsci.v33i2.7018

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    Rafael Arantes

    2011-05-01

    Full Text Available A simulation was conducted to determinate the impact caused by the combination of Litopenaeus vannamei respiratory rate (mg O2 shrimp-1 h-1, the behavior of SOTR (kg O2 h-1 of mechanical aerators as a function of salinity, as well as the oxygen consumption rate of the pond water and soil (mg O2 L-1 h-1 on the aeration of shrimp ponds (1, 10, 50 and 100 ha stocked with different densities (10, 40 and 120 shrimp m-2, salinities (1, 13, 25 and 37 ppt, temperatures (20, 25 and 30°C, and shrimp wet weight (5, 10, 15 and 20 g. Results showed that under lower salinity, with larger shrimp, and higher stocking density, higher will be the quantity of required 2-HP aerators to keep dissolved oxygen over 50% saturation. In addition, under low salinity, with 5 and 10 g shrimp, independent of stocking density, more aerators per hectare are required and electricity cost is higher at 20°C and salinity 1 ppt. Less aerators and lower electricity cost was observed at 30°C, salinities of 25 and 37 ppt, and shrimp of 15 and 20 g.A simulation was conducted to determinate the impact caused by the combination of Litopenaeus vannamei respiratory rate (mg O2 shrimp-1 h-1, the behavior of SOTR (kg O2 h-1 of mechanical aerators as a function of salinity, as well as the oxygen consumption rate of the pond water and soil (mg O2 L-1 h-1 on the aeration of shrimp ponds (1, 10, 50 and 100 ha stocked with different densities (10, 40 and 120 shrimp m-2, salinities (1, 13, 25 and 37 ppt, temperatures (20, 25 and 30°C, and shrimp wet weight (5, 10, 15 and 20 g. Results showed that under lower salinity, with larger shrimp, and higher stocking density, higher will be the quantity of required 2-HP aerators to keep dissolved oxygen over 50% saturation. In addition, under low salinity, with 5 and 10 g shrimp, independent of stocking density, more aerators per hectare are required and electricity cost is higher at 20°C and salinity 1 ppt. Less aerators and lower electricity cost was

  3. Efeito da adição do melaço na relação carbono/nitrogênio no cultivo de camarão Litopenaeus vannamei na fase berçário - DOI: 10.4025/actascibiolsci.v31i4.4496 Effect of molasses addition on carbon/nitrogen ratio in the nursery phase of Litopenaeus vannamei shrimp culture - DOI: 10.4025/actascibiolsci.v31i4.4496

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    Diogo Bessa Neves Spanghero

    2009-08-01

    Full Text Available Investigou-se o efeito da adição do melaço nas relações carbono:nitrogênio (C:N sobre o desempenho zootécnico do camarão Litopenaeus vannamei, na fase berçário, quando cultivado sem renovação de água. As relações do C:N foram avaliadas nas proporções de 25:1 (25M, 15:1 (15M e o controle (0M, sem aplicação de carbono, em delineamento experimental inteiramente casualizado, com quatro repetições. Pós-larvas (PL com peso inicial de 2,5 ± 0,5 mg, foram estocadas em 12 tanques (800 L volume útil, em densidades de 6,25 PL L-1, durante 42 dias de cultivo. Ao final do cultivo, os pesos finais dos camarões dos tratamentos 25M (532,0 mg e 15M (540,0 mg foram superiores (p L. vannamei cultivadas na fase berçário sem renovação de águaThe effect of molasses addiction on carbon:nitrogen ratios (C:N on the performance of Litopenaeus vannamei shrimp during the nursery phase cultured without water exchange was investigated. The C:N ratios were evaluated in 25:1 (25M and 15:1 (15M proportion and a control (0M, with no carbon source addition, in a randomized experimental design with four replicates. Post-larvae (PL with initial weight of 2.5 ± 0.5 mg were stocked in 12 tanks (800 L net volume, at the density of 6.25 PL L-1 during 42 culture days. At the end of culture, the shrimps weights on 25M (532.0 mg and 15M (540.0 mg treatments were higher (p L. vannamei post-larvae cultured without water exchange.

  4. Studies on early development and larval morphology of pond-reared blue shrimp Litopenaeus stylirostris%细角滨对虾的幼体发育及形态特征

    Institute of Scientific and Technical Information of China (English)

    许尤厚; 胡超群; 张吕平; 陈国良; 吴立峰

    2007-01-01

    以细角滨对虾Litopenaeus stylirostris为实验对象,按时间顺序观察了胚胎各期的形态特征.通过解剖从无节幼体到仔虾第1天各期幼体的附肢,对其发育形态及其刚毛着生方式进行了描述和统计.比较研究了细角滨对虾和凡纳滨对虾L.vannamei无节幼体期形态差异以及色素的变化、溞状幼体期眼上棘形态的差异以及大颚齿数目.结果表明,无节幼体期细角滨对虾的色素明显多于凡纳滨对虾;两者溞状幼体期眼上棘的形态存在明显差异;两者在无节幼体至仔虾第1天各期大颚活动齿的数目变化一致.

  5. 凡纳滨对虾肠道微生物宏基因组 Solexa 测序及其初步分析%Solexa sequencing and analysis of the intestinal microorgan-isms metagenome in Litopenaeus vannamei

    Institute of Scientific and Technical Information of China (English)

    严雪平; 袁剑波; 刘斌

    2012-01-01

      CTAB-酚/氯仿法提取新鲜凡纳滨对虾(Litopenaeus vannamei)肠道微生物宏基因组 DNA.结果表明: CTAB-酚/氯仿法提取总 DNA 的浓度达到92.5 ng/μL,半定量 PCR 法检测微生物基因组相对含量为69.9%.凡纳滨对虾肠道微生物宏基因组用于 Solexa 测序,生物信息学分析结果显示宏基因数据中64.1%的数据属于未知序列,35.5%属于真核生物,而已知的微生物和病毒序列所占比例仅有0.4%

  6. An in vitro study of alkaline phosphatase sensitivity to mixture of aflatoxin B1 and fumonisin B1 in the hepatopancreas of coastal lagoon wild and farmed shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Pérez-Acosta, Jesús A; Burgos-Hernandez, Armando; Velázquez-Contreras, Carlos A; Márquez-Ríos, Enrique; Torres-Arreola, Wilfrido; Arvizu-Flores, Aldo A; Ezquerra-Brauer, J Marina

    2016-08-01

    This study aimed to establish the combined effect of aflatoxin B1 (AFB1) and fumonisin B1 (FB1) on wild Litopenaeus vannamei hepatopancreas alkaline phosphatase (AP) activity compared with that of farmed shrimp. AP activity in hepatopancreas extract was confirmed by several specific inhibitor assays. AP activity of wild shrimp was higher than that of farmed shrimp (p shrimp was inhibited when incubated with AFB1 and FB1. The greatest inhibition occurred when AP was incubated with a mixture of AFB1 and FB1. The IC50 for AFB1 on AP activity of wild and farmed shrimp hepatopancreases was 0.790 and 0.398 μg/mL, respectively. The IC50 of FB1 was 0.87 μg/mL for wild shrimp and 0.69 μg/mL for farmed shrimp. These results suggest that, at the mycotoxins concentrations used in the study, AP from farmed L. vannamei was sensitive to the presence of both mycotoxins; however, AP is more sensitive to the combination of AFB1 + FB1 suggesting a possible synergistic or potentiating inhibitory effect.

  7. Comparison of Pollen Production and Quality Characteristics of Cultivated and Wild Almond Species Comparación de Producción y Características de Calidad del Polen de Especies de Almendro Cultivadas y Silvestres

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    Safder Bayazit

    2011-12-01

    breeding programs.Hay varias especies de almendros silvestres en Turquía incluidas Amygdalus orientalis (Mill., Amygdalus turcomanica (Lincz., Amygdalus fenzliana (Fritsch Lipsky, Amygdalus trichamygdalus (Hand.-Mazz. Woronow, Amygdalus arabica (Olivier, y Amygdalus webbii (Spach. Estas especies ofrecen un gran valor para mejoramiento del almendro; se estudió la viabilidad del polen, tasa de germinación, y producción de polen para siete genotipos de A. orientalis y siete genotipos de A. turcomanica cultivados en condiciones naturales en el sudeste de Anatolia (Gaziantep and §anhurfa provinces, Turkey. También se estudiaron cultivares de almendros (Prunus dulcis [Mill.] D.A. Webb obtenidos desde Pozanti Agricultural Experimental Station, Universidad de Cukurova. Las viabilidades de polen de los diversos genotipos se determinaron por las pruebas 2,3,5-trifeniltetrazolium cloruro (TTC y fluoresceína diacetato (FDA. Las tasas de germinación de polen se establecieron en condiciones de placas Petri (1% agar + 0, 10, 15, y 20% sucrosa mientras la producción de polen se estimó con métodos hemocitométricos. Las tasas de viabilidad de polen fueron cercanas entre ambos métodos para los genotipos de A. orientalis y A. turcomanica. Las tasas de germinación de polen fueron dependientes del contenido de sucrosa y del genotipo. Los cultivares mostraron tasas de germinación de polen similares en todas las concentraciones de sucrosa mientras el polen de los genotipos A. orientalis y A. turcomanica mostraron mayores tasas de germinación en 10% sucrosa. El número de anteras por flor fue mayor en los cultivares mientras el número de granos de polen fue menor en otras especies de almendro. Mientras el número de granos de polen por flor fue relativamente alto en genotipos de A. orientalis, la calidad del polen fue alta en las tres especies en investigación. Los resultados sugirieron que estas dos especies, A. orientalis y A. turcomanica, podrían ser usadas para futuros

  8. Parasites of domestic and wild canids in the region of Serra do Cipó National Park, Brazil Parasitos de canídeos domésticos e silvestres na região do Parque Nacional da Serra do Cipó - Brasil

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    Juliana Lúcia Costa Santos

    2012-09-01

    Full Text Available Over recent decades, diseases have been shown to be important causes of extinctions among wild species. Greater emphasis has been given to diseases transmitted by domestic animals, which have been increasing in numbers in natural areas, along with human populations. This study had the aim of investigating the presence of intestinal helminths in wild canids (maned wolf, Chrysocyon brachyurus, and crab-eating fox, Cerdocyon thous in the Serra do Cipó National Park (43-44º W and 19-20º S and endo and ectoparasites of domestic dogs in the Morro da Pedreira Environmental Protection Area (an area surrounding the National Park. The Serra do Cipó is located in the state of Minas Gerais, Brazil. Among the enteroparasites found in domestic and wild canids, the following taxons were identified: Ancylostomidae, Trichuridae, Toxocara sp., Spirocerca sp., Physaloptera sp., Strongyloides sp., Cestoda, Dipylidium caninum, Diphyllobothriidae, Hymenolepidae, Anoplocephalidae, Trematoda, Acanthocephala and Isospora sp. Domestic dogs were positive for leishmaniasis and Babesia canis in serological tests. Among the ectoparasites, Rhipicephalus sanguineus, Amblyomma cajennense and Ctenocephalides felis felis were observed in domestic dogs. Variations in the chaetotaxy of the meta-episternum and posterior tibia were observed in some specimens of C. felis felis.Nas últimas décadas, as doenças têm sido apontadas como importantes causas de extinção de espécies silvestres. Maior ênfase tem sido dada às doenças transmitidas por animais domésticos que crescem em número, bem como as populações humanas, em áreas naturais. O presente estudo objetivou verificar a presença de helmintos intestinais de canídeos silvestres (lobo-guará - Chrysocyon brachyurus e cachorro-do-mato - Cerdocyon thous do Parque Nacional da Serra do Cipó (43-44º W e 19-20º S e endo e ectoparasitos de cães domésticos da Área de Proteção Ambiental Morro da Pedreira (entorno do

  9. 密度胁迫对凡纳滨对虾稚虾免疫指标及生长的影响%Effects of stocking density on immune parameters and growth of juvenile Litopenaeus vannamei

    Institute of Scientific and Technical Information of China (English)

    张华军; 李卓佳; 张家松; 张晓阳; 曹煜成; 文国樑; 程开敏

    2012-01-01

    将体长为(3.59±0.26)cm的凡纳滨对虾(Litopenaeus vannamei)按1800尾·m-3、1 500尾·m-3和1200尾·m-3的密度分别饲养在注水0.3 m3的圆形玻璃纤维桶(容量为0.5 m3)中30 d,检测了基本水质因子、对虾肝胰腺和肌肉组织中的酚氧化酶(PO)活力、超氧化物歧化酶(SOD)活力、抗菌活力(Ua)、碱性磷酸酶(AKP)活力、体质量增长和成活率,研究了不同放养密度对凡纳滨对虾稚虾免疫指标及生长的影响.统计分析发现,试验过程中水体理化因子均在适宜范围,各处理间水质因子的差异不显著;对虾的PO活力、Ua和AKP活力均随着密度的增加而降低,SOD活力则反之;对虾的体质量增长、体质量特定增长率和成活率随着密度的增加而降低,成活率差异显著(P<0.05).结果表明,凡纳滨对虾(体长<4.8 cm或体质量<1.2g)在密度为1 200~1 800尾·m-3时,密度胁迫可明显影响其免疫指标和生长.%In order to investigate the effects of different stocking density on the immune parameters and growth of juvenile Litopenaeus vannamei, we cultured the shrimps [ body length (3. 59 ± 0. 26) cm ] in 0. 3 m3 fiberglass tanks ( water volume 0. 5 m3 ) at 1800 ind ·m-3, 1500 ind·m-3 and 1200 ind·m-3, respectively, for 30 d to determine the phenoloxidase (PO) activity, superoxide dis-mutase (SOD) activity, antibacterial activity (Ua) , alkaline phosphatase (AKP) activity in hepatopancreas and muscle, as well as weight gain, survival and basic water quality factors. It is showed that the physical and chemical factors vary within suitable range with no significant difference. The PO activity, Ua and AKP activity in the muscle of shrimp reduce while SOD activity increases with the increase of stocking density. The weight gain, special weight growth rate ( SGRw ) and survival reduce with the increasing stocking density with significant difference in survival (P<0. 05 ). The results indicate that stocking density may obivously

  10. Flebotomíneos coletados em matas remanescentes e abrigos de animais silvestres de zoológico no perímetro urbano de Maringá, sul do Brasil. Estudo preliminar Phlebotomines collected in remaining florests and wild animal shelters in zoological garden in the urban area of Maringá, Paraná State, shoutern Brazil. Preliminary study

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    Ueslei Teodoro

    1998-12-01

    Full Text Available Um caso de leishmaniose tegumentar com provável infecção em uma das áreas de matas remanescentes no perímetro urbano de Maringá, Paraná, Brasil, o desconhecimento da fauna e do comportamento de flebotomíneos nestas matas despertaram o interesse desta investigação. Os flebotomíneos foram coletados com armadilhas de Falcão instaladas em matas remanescentes do Parque do Ingá, Bosque Dois e Horto Florestal, de junho a setembro de 1995, no período noturno. Nestas áreas coletaram-se 3.532 flebotomíneos, prevalecendo Lutzomyia (Nyssomyia whitmani (Antunes & Coutinho, 1939 com 3.395 (96,1% exemplares. No Parque do Ingá, onde as coletas também foram feitas em abrigos de animais silvestres, mantidos em zoológico, foram coletados 2.907 flebotomíneos, dos quais 1.723 nestes abrigos. Os resultados mostram melhor adaptação de Lutzomyia whitmani nas matas remanescentes no perímetro urbano de Maringá e sua freqüência nos abrigos dos animais silvestres mantidos em cativeiro.The present study was undertaken after the detection of one case of cutaneous leishmaniasis with presumed infection in one of the three remaining wooded areas in the urban area of the city of Maringá, Southern Brazil; also in view of the lack of knowledge about sand flies and their behavior. From June to September, 1995, sand flies were caught with Falcão traps during the night in the remaining wooded areas (Parque do Ingá, Bosque Dois and Horto Florestal. A total of 2,907 sand flies were caugth in Parque do Ingá; 1,723 of them were aught in forest traps and 1,184 in wild animal shelter traps at the zoo. The results show that Lutzomyia whitmani is better adapted to the three areas under study and that it frequently occurs in wild animal shelters within the urban perimeter of Maringá.

  11. Evidence of hantavirus infection in wild rodents captured in a rural area of the state of São Paulo, Brazil Evidência de infecção por hantavírus em roedores silvestres capturados em uma área rural do estado de São Paulo, Brasil

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    Elba Regina S. de Lemos

    2004-06-01

    Full Text Available Hantaviruses are the etiological agents of the Hantavirus Cardio-Pulmonary Syndrome, a serious rodent-borne disease in Brazil. In order to investigate the occurrence of hantavirus infection in wild rodents, a survey was conducted in three different suburban areas of the municipality of Pedreira, State of São Paulo, Brazil. Of the 145 wild animals captured belonging to 12 different species identified by morphology and karyological analysis, 107 were rodents of the following species: Akodon montensis, Bolomys lasiurus, Calomys tener, Oligoryzomys nigripes, Oligoryzomys flavescens, and Myocastor coypus. Blood samples from these rodents were assayed for the presence of antibodies against hantavirus by IgG ELISA using Andes recombinant nucleocapsid antigen. Antibody reactive to Andes virus was found in two different species, O. nigripes and O. flavescens. These results indicate a potential risk for hantavirus transmission to humans in this area, where reservoir rodents are present in peridomestic settings.Hantavírus é o agente etiológico da síndrome cardio-pulmonar por hantavirus (SCPH, uma importante doença transmitida por roedores no Brasil. Com o objetivo de se conhecer a ocorrência de infecção por hantavírus em pequenos roedores silvestres, uma análise sorológica foi conduzida em três diferentes áreas do subúrbio da cidade de Pedreira, São Paulo. Dos 145 animais silvestres capturados, pertecentes a 12 diferentes espécies identificadas por morfologia e por análise cariológica, 107 eram roedores das seguintes espécies: Akodon montensis, Bolomys lasiurus, Calomys tener, Oligoryzomys nigripes, Oligoryzomys flavescens, e Myocastor coypus. Amostras de sangue desses roedores foram testadas para a presença de anticorpos IgG contra o antígeno do nucleocapsídeo do vírus Andes através do teste ELISA. Reatividade sorológica ao vírus Andes foi observada em duas espécies diferentes, O. nigripes and O. flavescens. Estes resultados

  12. Whitefly species (Hemiptera: Aleyrodidae on wild and cultivated plants in the horticultural region of Rosario, Santa Fe, Argentina Especies de moscas blancas (Hemiptera: Aleyrodidae sobre plantas silvestres y cultivadas en la región hortícola de Rosario, Santa Fe, Argentina

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    Gustavo G. Gonsebatt

    2012-06-01

    Full Text Available Whiteflies of economic importance are polyphagous, being able to develop on a large number of cultivated and spontaneous plants. We recorded the whitefly species on vegetable and flower crops and the wild plants associated, under greenhouse and field conditions, for two years. We observed two species: Trialeurodes vaporariorum (Westwood and the Bemisia tabaci complex (Gennadius. T vaporariorum was recorded on 24 plant species (11 families, 12 and 8 of which are new hosts in Argentina and in the world, respectively. The B. tabaci complex was recorded only on flower production systems, on 19 plant species (11 families, 14 and 7 of which are new hosts in Argentina and in the world, respectively. The crops Glycine max (L. and Chrysanthemum morifolium Ramat., the wild species Amaranthus blitum L., Amaranthus quitensis Kunth, Conyza bonariensis (L., Galinsoga parviflora Cav., Sonchus oleraceus L. and Wedelia glauca (Ortega O. Hoffm. ex Hicken were hosts of both species. The only parasitoid recorded was Eretmocerus californicus near corni Haldeman (Hymenoptera: Aphelinidae from T. vaporariorum. This study, which is the first systematic survey of host plants in the region, intends to provide a better knowledge of the range of whiteflies host plants in Argentina.Las moscas blancas de importancia económica son polífagas y capaces de desarrollarse sobre numerosas plantas cultivadas y espontáneas. Registramos las especies de moscas blancas sobre cultivos hortícolas y de flores, y sobre las plantas silvestres asociadas. Observamos dos especies: Trialeurodes vaporariorum (Westwood y el complejo Bemisia tabaci (Gennadius. T. vaporariorum fue registrada sobre 24 especies de plantas (11 familias, 12 y 8 de las cuales son hospedantes nuevos para Argentina y a nivel mundial, respectivamente. El complejo B. tabaci fue registrado solo en sistemas de producción de flores, sobre 19 especies de plantas (11 familias, 14 y 7 de las cuales son nuevos hospedantes para

  13. Efecto del fotoperiodo y la razón camarón:macroalga en la remoción de nitrógeno amoniacal total por Gracilaria vermiculophylla, en cultivo con Litopenaeus vannamei, sin recambio de agua

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    Adolfo Sánchez-Romero

    2013-11-01

    Full Text Available Se evaluó el efecto del fotoperiodo y la relación de biomasa camarón:macroalga en la remoción de nitrógeno amoniacal total (NAT, por la macroalga Gracilaria vermiculophylla, en cultivo con camarón Litopenaeus vannamei, sin recambio de agua. Se evaluaron cuatro fotoperiodos: 12:12, 14:10, 16:08 y 24:00 h de luz:oscuridad y dos relaciones de biomasa camarón:macroalga, 1:4 y 1:8. El diseño experimental fue factorial de 4x2 con cuatro réplicas por tratamiento. Las menores concentraciones de NAT (0,55 ± 0,17 mg L-1, N-NO² (0,145 ± 0,29 mg L-1 y N-NO3 (3,13 ± 0,73 mg L-1 se registraron en la relación 1:8. Las mayores tasas de crecimiento de la macroalga (3,07 ± 1,44% día-1 se obtuvieron con fotoperiodo de 24:00 h y relación de biomasa 1:8. La mayor tasa de incorporación de N en la macroalga (0,31 ± 0,12 mg N L-1 día-1 ocurrió en las mismas condiciones. El crecimiento del camarón fue similar al reportado en sistemas tradicionales de recirculación. La integración de G. vermiculophylla en el sistema sin recambio, fue capaz de mantener los niveles de compuestos nitrogenados tóxicos en niveles favorables para el crecimiento de L. vannamei.

  14. Comparative analysis of water quality in Litopenaeus vannamei ponds and nutritional quality of shrimp muscle%凡纳滨对虾池塘水质及对虾肌肉品质的对比分析

    Institute of Scientific and Technical Information of China (English)

    李世凯; 江敏; 戴习林; 刘利平; 胡伟国; JAMES S. DIANA

    2012-01-01

    From May to September in 2010,water quality parameters such as water temperature,total dissolved salt,dissolved oxygen,pH,transparency,nitrite nitrogen,ammonia nitrogen,nitrate nitrogen,total nitrogen,reactive phosphorus,total phosphorus,chlorophyll-a and biochemical oxygen demand were analyzed in 22 Litopenaeus vannamei ponds with different culturing methods in Fengxian District,Shanghai.Water used for Farm No.1 was natural fresh water which had been precipitated before being introduced to the ponds.Mixed salt were added to the fresh water for culturing shrimp in Farm No.2.Results were as follows: water temperature,dissolved oxygen and pH didn't change dramatically and could match the demand of Litopenaeus vannamei.The proportion of nitrate nitrogen in ponds to TIN was the highest,the ratio of ammonia nitrogen to TIN increased with time extension,and that of nitrite nitrogen to TIN increased obviously in the later period of culture cycle.Contents of reactive phosphorus decreased gradually while the total phosphorus increased in the whole process of culture.Biochemical oxygen demand and chlorophyll-a also increased gradually with the shrimp growing up.Muscle nutritional quality of Litopenaeus vannamei from the two farms were analyzed and the results were as follows: contents of crude protein and crude fat of shrimp muscle in Farm No.1 were 16.30% and 1.42% respectively,lower than those in shrimp muscle from Farm No.2 which were 18.30% and 1.61%.Content of total amino acids in shrimp from Farm No.1was 23.27%,and the essential amino-acid was 9.09%.While those in shrimp from Farm No.2 were 27.52% and 10.74%.Contents of flavor amino acids in shrimp from Farms No.1 and 2 were 8.52% and 10.16% respectively.%2010年5-9月期间,对上海奉贤区两个养殖场采取不同养殖方式的22个凡纳滨对虾养殖塘的水温(T)、总溶解盐(TDS)、溶解氧(DO)、pH、透明度(SD)、亚硝酸盐氮(NO2-N)、总氨氮(TAN)、硝酸盐氮(NO3-N

  15. Estudo da infecção esquistossomótica produzida pelas linhagens humana e silvestre do Vale do Rio Paraíba do Sul, SP (Brasil, em camundongos isogênicos Some results of schistosomiasis mansoni in inbred mice infected with human and wild rodent strains from the Paraiba do Sul River Valley, S. Paulo, Brazil

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    Othon de Carvalho Bastos

    1979-12-01

    Full Text Available Foi estudada a influência da isogenicidade de camundongos de laboratório na capacidade de penetração de cercárias de Schistosoma mansoni de linhagem humana do Vale do Rio Paraíba do Sul, SP. Foram estudados, comparativamente, aspectos da biologia e da patogenia das linhagens humana e silvestre do S. mansoni do Vale do Rio Paraíba do Sul, SP (Brasil em camundongos isogênicos. Foi vantajoso o uso de camundongos isogênicos devido a maior homogeneidade dos resultados e a maior penetração de cercárias pelo tegumento da cauda dos roedores.The behavior of a human S. mansoni strain was studied in infected, outbred mice. A comparative study was carried out to evaluate the effects of the infection in inbred mice, employing human and wild rodent strains of S. mansoni. The use of inbred mice was advantageous in that it allowed greater uniformity in the results as well as greater penetration of surrounding areas through the caudal tegmentum.

  16. Isolation and Identification of Extracellular Enzyme-Producing Bacteria from the Intestinal Tract of Litopenaeus vannamei%具有多种胞外酶的对虾肠道黏附菌的分离和鉴定

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    刘慧玲; 罗鹏; 杨世平; 李广聪; 莫嘉文; 王蔚

    2012-01-01

    用对虾饲料培养基从健康凡纳滨对虾肠道分离出500株黏附细菌,以产淀粉酶、脂肪酶和蛋白酶能力为指标,筛选出产该3种消化酶的细菌90株,占总菌株的18%.对其中生长较快的69株进行16SrDNA基因测序,确定其分类地位.结果显示,69株菌分别属于不动杆菌属(Acinetobacter)、芽孢杆菌属(Bacillus)、葡萄球菌属(Staphylococcus)、假交替单胞菌属(Pseudoalteromonas)、气单胞菌属(Aeromonas)、嗜盐单胞菌属(Halomonas)、利斯顿氏菌属(Listonella)、莫拉氏菌属(Moraxella)等,其中数量最多是芽胞杆菌属,占鉴定细菌总数的53.62%,数量最少是气单胞菌属和嗜盐单胞菌属,均占鉴定细菌总数的2.90%.表明对虾肠道黏附菌群中具有较多能分泌多种消化酶的细菌,可进一步开发为促进对虾消化功能的益生菌.%500 strains adhesive bacteria were isolated from the intestinal tract of Litopenaeus vannamei by using shrimp-feed abstraction medium. All bacterial strains were screened for producing capability of protease, amylase and lipase. There were 90 strains that could produce three extracellular enzymes, 18.0% of the total intestinal adhesive bacterial populations. 69 strains with rapid growth were identified according to homology analysis of 16S rDNA sequences. They were grouped into eight genera as follows: Acinetobacter, Bacillus, Staphylococcus, Pseudoalteromonas, Aeromonas, Halomonas, Listonella, Moraxella, respectively. The dominant genus was Bacillus, which account for 53.62% of the total identified strains. There were least number strains belong to Aeromonas and Halomonas, which account for 2.90% of the total identified isolates, respectively. The results indicate that there are plentiful extracellular enzyme-producing bacterium adhered to intestine of shrimp with the potential to be promising probiotic of promoting digestion.

  17. 在不同盐度下氯氰菊酯对凡纳滨对虾的急性毒性效应研究%Study on the Acute Toxicity of Cypermethrin on Litopenaeus vannamei in Different Salinity

    Institute of Scientific and Technical Information of China (English)

    邱勇; 严峰; 曾嶒; 熊泽泉

    2012-01-01

    [目的]为不同盐度下氯氰菊酯的使用和调控提供科学依据.[方法]采用静水生物法,研究了不同盐度下氯氰菊酯对凡纳滨对虾的急性毒性效应.[结果]在盐度20和5下氯氰菊酯对凡纳滨对虾的24、48、72、96h半致死浓度(LC50)分别为0.767、0.440、0.383、0.038 2 μg/L和0.437、0.313、0.203、0.170 μg/L.在中、低盐度下氯氰菊酯对凡纳滨对虾的安全浓度(SC)分别为0.038 2和0.017 0μg/L.[结论]极低浓度的氯氰菊酯农药残留就可能会威胁凡纳滨对虾的养殖安全,应尽可能减少在虾塘及附近使用氯氰菊酯.%[Objective] The research aimed to provide scientific basis for the use and control of cypermethrin in different salinity. [ Method] U-sing static bioassay test, the acute toxicity effect of cypermethrin on Litopenaeus vannamei in different salinity were studied. [ Result ] 24, 48, 72, 96 h LCX of cypermethrin to L vannamei in the salinity of 20 and 5 were 0.767, 0.440, 0.383, 0.038 2 |xg/L and 0.437,0.313,0. 203 and 0.170 fig/L respectively. In medium and low salinity, the safe concentration of cypermethrin to L. vannamei was 0.038 2 and 0.017 0 p.g/L respectively. [Conclusion] Very low concentrations of cypermethrin pesticide residues might threaten the safety of L. vannamei culture, so the use of cypermethrin should be reduced as possible in shrimp pond and its surrounding area.

  18. 凡纳滨对虾对虾素3a基因的克隆与序列分析%Gene Cloning and Sequence Analysis of Penaeidin 3a in Litopenaeus vannamei

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    杜志强

    2013-01-01

    Antibacterial peptide molecules are the main immunity effectors in the innate immunity system of the low invertebrate. They play an important part in preventing pathogens invasion. In order to explore the antibacterial peptides molecular diversity and the molecular structure association, we took Litopenaeus vannamei as the study object. Gene cloning and structure analysis of penaeidin 3a antibacterial peptides gene were done. The results indicated that penaeidin 3a molecular possessed classic shrimp penaeidin domain. And there was a spherical structure that formed by eight conserved cysteine residues. Especial-ly , there was a pro-rich motif which played an important role in forming the special molecular function. This study could provide the theoretical basis for the next functional research of penaeidin molecular.%抗菌肽分子是无脊椎动物先天免疫系统中的主要免疫效应分子,在防止病原菌入侵方面,发挥着重要作用.为了探索先天免疫系统中抗菌肽分子的多样性以及分子结构之间的关联,本文以凡纳滨对虾为研究对象,对其enaeidin 3a抗菌肽基因进行了基因克隆和生物信息学分析.研究结果表明penaeidin 3a分子具有经典的对虾素结构域,而且存在着一个由8个保守的半胱氨酸残基形成的球状实体结构,尤其是分子中存在富含脯氨残基的结构基序,对于分子功能的形成具有重要作用.本研究可为下一步对虾素分子功能的研究提供理论基础.

  19. 凡纳滨对虾全同胞家系的建立及生长比较%Full-sib families construction and their growth comparison of Pacific white leg shrimp, Litopenaeus vannamei

    Institute of Scientific and Technical Information of China (English)

    张吕平; 吴立峰; 沈琪; 杜少波; 胡超群

    2009-01-01

    全同胞家系是遗传分析的重要材料.采用自然交配法建立了62个凡纳滨对虾第一代全同胞家系,对其中17个家系进行了比较研究.结果表明,各家系的孵化率、出苗率与雌性亲本的大小相关性不显著.17个家系中有7个家系显示生长优势,其大小顺序依次是A02>A11>A01>A17>A06>A08>A16;7个家系在40周内的生长速度较17个家系的平均值高23.2%.在所有家系中,A01、A02、A11等3个家系的体重显著大于其它家系(P A11 > A01 > A17 > A06 > A08 > A16. These seven faster families are 23.2% higher than the mean value of all 17 families in growth rate (g/week) during the 40 weeks traits (P <0. 05). By comparison of each family, families A01, A02 and All are 25.5% , 31.6% and 31. 0% larger than the mean value of the seventeen families respectively. Moreover, these three families display the smaller coefficient of variation (CV), and the CV values in the middle of growth period are 11. 58% , 9.95% and 8.48% respectively. The CV values of the three families are <5% in the later growth period. Therefore the results indicate that they are three families with excellent economic properties. These results are helpful for further selective breeding of fast-growth line of Litopenaeus vannamei.

  20. Gene expression profiles of Litopenaeus vannamei in response to carbonate alkalinity stress%碳酸盐碱度胁迫下凡纳滨对虾基因的差异表达

    Institute of Scientific and Technical Information of China (English)

    么宗利; 应成琦; 周凯; 王慧; 来琦芳

    2012-01-01

    以广盐性养殖的凡纳滨对虾(Litopenaeus vannamei)为研究对象,采用抑制消减杂交(suppression subtractive hybridization,SSH)和定量PCR的方法,研究其在高碳酸盐碱度胁迫下转录组水平的基因表达差异,以期从基因组水平研究对虾对高碳酸盐碱度胁迫的适应机制.结果表明,以高碳酸盐碱度(20 mmol/L)胁迫第4天凡纳滨对虾鳃组织和低碱度(2mmol/L)对照组鳃组织为材料,通过斑点杂交筛选,发现鳃组织中有158个克隆子表达上调,291个克隆子表达下调.挑选150个高表达差异的克隆子进行测序,获得100个序列,其中50个得到成功注释.经过GO分析,这些注释的差异基因主要分为8大类群,其中碳酸酐酶基因(CA)、Na+-K+-ATPase基因(NKA-α)等与离子调控相关的基因表达量上调,而溶菌酶基因等与先天免疫相关的基因表达量下调,这些结果表明高碳酸盐碱度胁迫下,凡纳滨对虾以增加离子调控的方式进行酸碱平衡的调控,同时其免疫功能受到抑制.此外,还对CA和NKA-α两个基因在鳃和触角腺中的时空表达规律进行了研究,发现高碳酸盐碱度胁迫9d过程中,两个基因在鳃组织中的表达均呈现先高表达后回落的现象,而在触角腺中NKA-α基因则一直维持较高表达水平,说明CA和NKA-α基因在凡纳滨对虾高碳酸盐碱度适应离子调控中起着关键作用,同时还发现除了鳃组织之外,触角腺同样参与了调控.本研究从转录水平初步筛选了高碳酸盐碱度胁迫下凡纳滨对虾的表达差异基因,探索了凡纳滨对虾的耐盐碱机制,对培育适于盐碱地水产养殖的优良品种有着重要的意义.%Saline-alkali water-bodies are common in China. Alkalinity stress is considered to be one of the primary stressors for shrimp in saline-alkali water. Thus, an improved understanding of the molecular response to alkalinity stress is critical for advancing the sustainability of shrimp culture

  1. Composition of the essential oil from the leaves of tree domestic varieties and one wild variety of the guava plant (Psidium guajava L., Myrtaceae Avaliação dos constituintes dos óleos essenciais de folhas de goiabeira (Psidium guajava L., Myrtaceae de três cultivares e da planta silvestre

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    Rafaela Karin de Lima

    2010-03-01

    Full Text Available The compositions of the essential oils from the leaves of three domestic varieties of the guava tree Psidium guajava L. (Paluma, Século XXI and Pedro Sato and of one wild variety were compared. Essential oils were extracted by steam distillation, the components were identified by gas chromatography coupled to mass spectrometry GC-MS, and the apparent concentrations were determined by gas chromatography with a flame ionization detector. The results demonstrated that the three essential oils contained many common substances with a prevalence of 1,8-cineole, whereas the essential oil of the Paluma variety contained 1,8-cineole (42.68% as the major constituent, as well as α-terpineol (38.68%. The principal components of the essential oil of the Século XXI variety were 1,8-cineole (18.83%, trans-caryophyllene (12.08%, and selin-11-en-4-αol (20.98%, while those of the Pedro Sato variety and of the wild plant were 1,8-cineole (17.68% and (12.83%, caryophyllene oxide (9.34% and (9.09%, and selin-11-en-4-α-ol (21.46% and (22.19%, respectively.Os óleos essenciais de folhas de goiabeira Psidium guajava L. de três diferentes cultivares (Paluma, Século XXI e Pedro Sato e da planta silvestre foram analisados a fim de comparar os seus constituintes. O óleo essencial foi obtido por hidrodestilação em aparelho de Clevenger modificado. Para a caracterização e quantificação dos compostos presentes por CG-EM e CG respectivamente. Os resultados mostraram que os três óleos essenciais analisados apresentam inúmeras substâncias em comum com a predominância do 1,8-cineol. O óleo essencial da cultivar Paluma se difere dos demais devido a alta concentração de 1,8-cineol (42,68% e do α-terpineol (38,68%. No óleo essencial da cultivar Século XXI observase a presença do 1,8 cineol (18,83%, trans-carifileno (12,08% e o selin-11-en-4-α-ol (20,98% como majoritários, enquanto que a cultivar Pedro Sato e a planta silvestre apresentaram com o 1

  2. Absconding and migratory behaviors of feral Africanized honey bee (Apis mellifera L. colonies in NE Brazil = Comportamentos de abandono e migração de colônias silvestres da abelha melífera africanizada (Apis mellifera L. no nordeste do Brasil

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    Breno Magalhães Freitas

    2007-10-01

    Full Text Available We investigated the annual movements of feral Africanized honey bee (Apis mellifera L. colonies in the state of Ceará, Brazil, aiming to understand seasonal variations in their population. Arrival and absconding of Africanized honey bee (AHB colonies in the semiaridmunicipality of Canindé and the coastal humid city of Fortaleza (120 km apart were recorded weekly from January 1999 to December 2001, and the data compared to rainfall records in both areas. Results showed that AHB colonies only nest in the semiarid during the rainy season and abscond during the dry season, the opposite from observations taken in Fortaleza. Only 5% of colonies remained in the semiarid area for the entire year due to ant (Camponotus sp. attacks and shortage of nectar and water during the dry season, with most colonies migrating to coastal areas where the weather is milder and many plant species bloom at that time of year. Excessive rainfallprobably pushes AHB colonies back to the semiarid during the rainy season. We concluded that absconding and migration are strategies that allow AHB colonies to survive in the semiarid NE of Brazil, contrary to European honeybees, which have never succeeded in establishing wild colonies in the region.Os movimentos de colônias silvestres da abelha melífera africanizada (Apis mellifera L. no Estado do Ceará, Brasil, foram investigados com o objetivo de compreender variações anuais em sua população. A chegada e a partida de colônias de abelhas africanizadas (AHB,no município semi-árido de Canindé e na úmida cidade litorânea de Fortaleza (separadas por 120 km, foram monitoradas semanalmente, de janeiro de 1999 a dezembro de 2001, e comparados com os dados pluviométricos de chuvas em ambas as áreas. Os resultadosdemonstraram que as abelhas africanizadas somente nidificaram no semi-árido durante a estação chuvosa e o abandonaram na estação seca, ao contrário do observado em Fortaleza. Apenas 5% das col

  3. Impactos de atropelamentos de animais silvestres no trecho da rodovia SP-215 confrontante ao Parque Estadual de Porto Ferreira – Porto Ferreira, SP (Nota Científica. Impacts roadkills mortality of wild animals in the area of SP-215 highway alongside Porto Ferreira State Park – Porto Ferreira, SP (Scientific Note.

    Directory of Open Access Journals (Sweden)

    Sonia Aparecida de SOUZA

    2010-12-01

    Full Text Available No presente trabalho foi realizado o monitoramento dos atropelamentos de animais silvestres na rodovia SP–215 no trecho confrontante ao Parque Estadual de Porto Ferreira. Em seis anos foram registrados 72 indivíduos, sendo 17 espécies de mamíferos, 10 de aves, quatro de répteis, uma de anfíbio e três indivíduos não identificados. Dentre esses, lobo-guará Chrysocyon brachyurus, jaguatirica Leopardus pardalis e cuíca-lanosa Caluromys lanatus se encontram na lista das espécies ameaçadas de extinção do Estado de São Paulo. A partir do conhecimento das espécies atingidas, faz-se necessário o desenvolvimento de estudos e propostas de implantação de medidas mitigadoras à presença da rodovia SP–215 para a conservação da fauna.This paper presents the data collected through the monitoring of roadkills on SP–215 highway in the area alongside Porto Ferreira State Park. During six years, 72 roadkills were recorded, including 17 species of mammals, 10 of birds, five of reptiles, one of amphibians and three unidentified individuals. Among those species, maned wolf Chrysocyon brachyurus, ocelot Leopardus pardalis, and Western woolly opossum Caluromys lanatus are included in the threatened species list of the state of São Paulo. After finding out which species are affected, it is necessary to develop studies and proposals for implementing effective mitigation measures for the presence of SP–215 highway in the area in order to preserve the local fauna.

  4. Infecções naturais de mamíferos silvestres em área endêmica de leishmaniose tegumentar do Estado de São Paulo, Brasil Natural infection of sylvatic mammals of cutaneous leishmaniasis in S. Paulo State, Brazil

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    Oswaldo Paulo Forattini

    1972-09-01

    Full Text Available Descreve-se a presença de foco de leishmaniose tegumentar no vale do rio Moji-Guaçú, em região comum aos municípios de Luiz Antonio, São Carlos, Rincão e Santa Rita do Passa Quatro, no Estado de São Paulo, Brasil. Os casos humanos apresentavam formas clínicas caracterizadas por lesões úlcero-vegetantes, de evolução lenta e pobres em parasitos. As investigações sobre infecção natural em animais silvestres levou ao isolamento de roedores, de três cepas em cultura, duas procedentes de Akodon arviculoides e uma de Oryzomys nigripes. As provas de inoculação em hamsters foram, até o momento, positivas para duas delas, mas com evolução lenta, com manifestações clínicas muito discretas e pobres em parasitos. Pelos dados disponíveis até o momento, parece tratar-se de cepas filiáveis à raça "lenta", à qual se atribui papel na etiologia da forma cutaneo-mucosa da leishmaniose.An endemic area of cutaneous leishmaniasis in the State of S. Paulo, Brazil, is described. Clinical aspects of human cases showed few lesions with slow evolution and few parasites. From investigations on natural infections of local sylvatic mammals by skin cultures technique ensued three strains isolations from Akodon arviculoides and Oryzomys nigripes rodents. Two of these showed to infect hamsters experimentally, but with discrete cutaneous manifestations and few parasites. This aspect seems to be alike the behaviour of "slow" strain, who was included in the possibility of the mucocutaneous leishmaniasis etiology. The presence of sylvatic enzootic forms of the disease is concluded.

  5. Isolation, identification, and pathogenicity analysis of a Vibrio parahaemolyticus strain from Litopenaeus vannamei%一株凡纳滨对虾病原菌的分离、鉴定及其致病力分析

    Institute of Scientific and Technical Information of China (English)

    张宝存; 刘飞; 边慧慧; 刘杰; 潘鲁青; 黄倢

    2012-01-01

    A pathogenic bacterium strain 20100612001 was isolated from the hepatopan-creas tissue of diseased white shrimp Litopenaeus vannamei.The LD50 challenge assay was performed and the LD50 value was found to be 1.44 ×106CFU/ml.The cell morphotype was observed to be bacilli,coccoid rod or pear-like and some cells could be observed intensely stained at two poles.The strain was Gram-negative) sporeless and had a single polar flagellum.It formed transparent or translucent round colonies on 2216E solid medium but green or blue-green colonies on TCBS medium.It showed the highest identity to V.parahaemolyticus or Vibrio natr-iegenis by using fatty acid analysis and Biolog system.Based on 16S rRNA sequencing,the strain is >98.9% identical to several species in Vibrio genus,and the phylogenetic tree revealed that it is most close to Vibrio parahaemolyticus.Taken together,the pathogenic strain 2010060612001 is identified as V.parahaemolyticus.%从患病凡纳滨对虾肝胰腺中分离出菌株20100612001,经人工感染实验证实,该分离菌株对凡纳滨对虾的半数致死量为1.44×106CFU/ml.形态学观察和革兰氏染色表明,该菌株为革兰氏阴性,无芽孢,有一根端极鞭毛;呈球杆状、球状、棒状或梨状且有两极浓染现象;在2216E培养基上为透明或半透明的圆形菌落,而在TCBS选择性培养基上为绿色或蓝绿色菌落.经Biolog碳源利用反应、脂肪酸气相色谱分析得出,该菌株与副溶血弧菌、需钠弧菌等的生理生化特性最相似;16S rDNA序列测定表明,该菌株与弧菌属中几株痛原菌的同源性均达到98.9%以上.在分子进化树中该菌株与副溶血弧菌Vibrio parahaemol yticus的进化地位最接近.综合上述实验结果分析得出,该细菌分离物为副溶血弧菌.

  6. cDNA Clone of Prophenoloxidase for Litopenaeus Stylirostris and Sequence Structure Analysis%细角滨对虾酚氧化酶原cDNA 克隆及序列结构分析

    Institute of Scientific and Technical Information of China (English)

    许尤厚; 胡超群

    2015-01-01

    采用 RT-PCR 原理和长片段扩增技术克隆细角滨对虾酚氧化酶原基因。结果表明,细角滨对虾血淋巴细胞内存在2个 proPO 基因。 proPO gene 1的 cDNA 序列包含有372氨基酸,前190个氨基酸为一个M 家族血蓝蛋白,是一个铜结合位点区域,191-372为一个 C 家族的血蓝蛋白,是一个免疫球蛋白样的区域。proPO gene 2的2个功能位点之间的序列有重叠,proPO gene 2 cDNA 序列的6-935bp 包含了第一个功能位点,928-1464bp 则包含了第二个功能位点。系统进化树比对分析发现2个基因之间的序列差异非常大。细角滨对虾和凡纳滨对虾的 proPO gene 2同处于一个密切相关的群,proPO gene 1则和其他几种对虾的 proPO gene 处于一个群。 proPO gene 2与 proPO gene 1在对虾免疫活动中是否存在不同的功能还有待于进一步的研究。%Prophenoloxidase (proPO) is one of the important factors on humoral immunity of shrimp, so far there are no re-ports for Litopenaeus stylirostris. Depend on techniques of RT-PCR and long fragment amplification cloning, prophenoloxidase gene of L. stylirostris was cloned. The results show that, there are two proPO genes in the lymphocytes of L. stylirostris. ProPO gene 1 cDNA sequence contains 372 amino acids, the first 190 amino acids are a family of M hemocyanin, a copper binding site region, 191-372 is one of the C family of hemocyanin, is an immunoglobulin like region. There are sequence overlap between the 2 functional sites of proPO gene 2, which means that 6-935bp contains the first functional sites, while 928-1464bp contains sec-ond functional sites. The phylogenetic tree alignment analysis showed that sequence structures of two genes is very different. Pro-PO gene 2 of L. stylirostris and L. vannamei was in a closely related group; but proPO gene 1 of L. stylirostris and L. vannamei was in another group with other several shrimp. The function of ProPO gene 2 and proPO gene 1 in shrimp immune

  7. Phytochemical Evaluation of Wild and Cultivated Pepper (Capsicum annuum L. and C. pubescens Ruiz & Pav. from Oaxaca, Mexico Evaluación Fitoquímica en Chile (Capsicum annuum L. and C. pubescens Ruiz & Pav. Silvestre y Cultivado en Oaxaca, México

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    Araceli Minerva Vera-Guzmán

    2011-12-01

    Full Text Available Reports of the last decade show that some types of food and spices included in the human diet, such as pepper (Capsicum annuum L. can have a positive effect on human health. The Mexican pepper germplasm is poorly documented with regard to variety and the amount of phytochemical compounds that it contains. In the present study, the variation of phytochemical compounds was evaluated in nine fruit variants (morphotypes of wild and cultivated pepper grown in Oaxaca. ANOVA detected significant differences among pepper morphotypes and ripeness stages of fruits; vitamin C, total phenols, flavonoids, P-carotene, coordinated chromatic of color, and capsaicinoids. The highest values of vitamin C were found in 'Tabaquero', 'Guero' and 'Costeño' morphotypes (151.6 to 183.2 mg 100 g-1. With regard to total phenols and flavonoids, 'Piquín' and 'Solterito' had the highest levels. Coordinates of color a* and b*, and chroma presented a positive correlation with phenol and flavonoid contents. The evaluated morphotypes differed in capsaicin and dihydrocapsaicin; C. annuum had higher capsaicin content (4.9 to 142 /En la última década, se reportó que el consumo de ciertos alimentos y especias, como el chile (Capsicum annuum L., pueden tener un efecto positivo en la salud. Particularmente, los acervos genéticos mexicanos de chile están poco documentados en relación a la diversidad desde la perspectiva fitoquímica. En este trabajo se evaluó la variación de compuestos fitoquímicos en nueve morfotipos de chile silvestres y cultivados de Oaxaca. El ANDEVA detectó diferencias significativas entre morfotipos y estados de madurez en vitamina C, fenoles, flavonoides, P-caroteno, color, y capsaicinoides. Los valores más altos de vitamina C se determinaron en 'Tabaquero', 'Guero' y 'Costeño' (151.6 a 183.2 mg 100 g-1. En fenoles y flavonoides sobresalieron los tipos 'Piquin' y 'Solterito'. Las coordenadas cromáticas a* y b*, y los tonos (C* se correlacionaron

  8. La educación e interpretación ambiental en las áreas silvestres protegidas del estado: La experiencia del Parque Nacional Conguillío (Región de La Araucanía para fomentar la conservación en los visitantes.

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    M. González

    2014-03-01

    Full Text Available Dentro de las Áreas Silvestres protegidas de la Región de la Araucanía, sur de Chile, se destaca el Parque Nacional Conguillío inserto en la precordillera andina. Considerado un área de gran importancia ecosistémica dada la diversidad de ambientes que presenta producto de la acción vulcanológica propia de esta cordillera, ésta unidad conforma parte del selecto grupo de Reserva de la Biosfera del país. En él es posible encontrar ecosistemas de bosque primario de Araucaria, humedales (incluyendo ríos, lagos y esteros, escoriales y altas cumbres como es el Volcán LLaima y Sierra Nevada, todas de importante valor científico, geológico, geomorfológico y turístico. Su alta concurrencia por sus atractivos escénicos, flora y fauna, generan una importante llegada de visitantes, nacionales y extranjeros especialmente en época estival dado las mejores condiciones metereológicas y la temporada de vacaciones. Es por ésta razón, que el programa de educación e interpretación ambiental creado pioneramente hace 37 años y actualmente vigente como actividades para sus visitantes adultos y niño(as, cobra un mayor sentido de pertenencia para el visitante a través de una experiencia en un ASPE. Las actividades como experiencia, buscan la generación de nuevas actitudes que involucren una conducta del visitante orientada a la sostenibilidad del parque y la apreciación del entorno natural como parte fundamental en el desarrollo del ser humano. En este contexto, el programa actual muestra un crecimiento sostenido de un 7% en número de visitantes entre temporada 2013-2014, siendo objetivo de este trabajo el mostrar antecedentes generales del parque, los senderos más visitados, las especies de flora y fauna más atractivas y/o con problemas de conservación, así como el programa en cuanto a las actividades y sus proyecciones de mejora.

  9. Comparação de métodos de laboratório e de campo para a estimativa da área foliar em fruteiras silvestres Comparison of laboratory and field methods for the estimation of leaf area of wild fruit species

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    M.S. Mielke

    1995-04-01

    Full Text Available Foi realizado um trabalho com o objetivo de estudar diferentes métodos de laboratório e de campo para a estimativa da área foliar de fruteiras silvestres, pertencentes à família Myrtaceae, a saber: uvalheira (Eugenia uvalha Camb., aracazeiro (Psidium cattleyanum Sabine, goiabeira serrana (Feijoa sellowiana Berg. e pitangueira (Eugenia uniflora DC. Entre os métodos de laboratório, também utilizados como "padrão", bons resultados foram obtidos utilizando-se um medidor automático de área foliar, planímetro ou através do método gravimétrico (fotocópia ou papel filtro. Dentre os métodos de campo, os melhores resultados foram obtidos por regressão linear. As estimativas das áreas foliares (y para as quatro espécies estudadas podem ser tomadas a partir das equações: y = 2,658 + 0,554X, para o araçazeiro; y = 0.75X ou y = 0,856X - 2,115, para a goiabeira serrana; y = 0.68X, para a uvalheira e y = 0,69X ou y = 0,503 + 0,643X, para a pitangueira, sendo X = comprimento x largura da folha.This work was carried out in order to study different laboratory and field methods for estimating leaf area of wild fruit species, belonging to the Myrtaceae: "uvalha" plant (Eugenia uvalha Camb., cattley guava plant (Psidium cattleyanum Sabine, feijoa plant (Feijoa sellowiana Berg and Surinam cherry plant (Eugenia uniflora DC. Among the laboratory' methods utilized as "standard methods", good results were obtained with the use of an automatic leaf area meter, planimeter or by the gravimetric method (photocopy or Whatman paper. Among field methods, best results were obtained with linear regression. The estimation of leaf area y for all studied species could be obtained through the equations: y = 2,658 + 0,554 x for cattley guava plant; y = 0,75 x or y = 0,856 x - 2,115 for feijoa plant; y = 0,68 x for "uvalha" plant and y = 0,69 x or y = 0,503 + 0,643 x for Surinam cherry plant, where x = leaf length x leaf width.

  10. 超高压联合高密度 CO2处理钝化对虾多酚氧化酶%Inactivation of polyphenol oxidase from Litopenaeus vannamei treated by ultra high pressure combined dense phase carbon dioxide

    Institute of Scientific and Technical Information of China (English)

    邓倩琳; 刘书成; 刘蒙娜; 刘媛; 郭明慧; 吉宏武; 李承勇; 高静

    2016-01-01

    Ultra high pressure (UHP) and dense phase carbon dioxide (DPCD) processes are effective non-thermal pasteurization methods that have gained increasing attention in inactivation of undesired enzymes and microorganisms in food industry. The advantage of UHP is to process foods that are already packaged and therefore are not liable to post-process contamination. Although UHP effectively eliminates microorganisms, it does not inactivate some key enzymes that reduce the product quality. For example, UHP may increase the activities of polyphenol oxidase (PPO) at lower pressure. As a continuous operation, DPCD needs aseptic filling to containers, but can inactivate enzymes. Therefore it is logical to combine these technologies to benefit from their individual advantages. The presence of carbon dioxide (CO2) in the sample medium might create a more acidic environment and synergistically interact with pressure to damage or alter the structures of enzymes and microbial cells. In order to make up for the disadvantage of UHP in inactivating PPO and use the advantage of DPCD in inactivating PPO, the inactivation effect of PPO from Litopenaeus vannamei treated by UHP combined with CO2 (UHP+CO2) was studied, and the feasibility of developing new shrimp products by UHP+CO2 was explored. The crude PPO extracts of 2 mL were treated with 2% CO2 (v/v) package alone, or UHP alone, or UHP + 2% CO2 (v/v). The treatment temperature was 30±2 ℃. The treatment pressure was 100, 200, 300, 400 and 500 MPa, respectively. The treatment time was 5, 10, 15, 20, 25 and 30 min, respectively. The results showed that: The PPO was inactivated more effectively by UHP+CO2 than CO2 treatment and UHP treatment alone. Treated at 100 MPa for 30 min by UHP+CO2, PPO activity dropped to 18.92%±1.52%. At 200 MPa for 10 min by UHP+CO2, PPO activity dropped to 10.91%±1.08%. At 300 MPa for 10 min by UHP+CO2, 95% PPO was inactivated. At 400 MPa for 5 min by UHP+CO2, the residual activity of PPO was less than 3

  11. Effects of Dietary Cornstarch Levels on Fat Metabolism of Hepatopancrease in Litopenaeus vannamei%不同玉米淀粉水平对凡纳滨对虾肝胰腺脂肪代谢的影响

    Institute of Scientific and Technical Information of China (English)

    郭冉; 刘永坚; 田丽霞; 夏辉; 王家庆

    2011-01-01

    采用8周的生长实验研究了以玉米淀粉为糖源的不同淀粉水平(w为10%、15%、20%、25%、30%、35%)对初始体质量为(0.96±0.02)g凡纳滨对虾的生长、体营养成分组成、肝胰脏显微结构和肝胰腺脂肪合成酶的影响.实验饲料中w(蛋白质)为38%;w(脂肪)=5%.实验在室内循环水族箱内进行,实验用水为天然咸淡水(盐度:6‰~14‰),6组饲料每组设3个重复,每箱30尾虾,饱食量投喂.实验结果表明:w(淀粉)=15%实验组对虾的增质量率、SGR最高,分别为453.62%和3.06,与w(淀粉)=10%和w(淀粉)=20%组无显著性差异,但明显高于其他各组;w(淀粉)=10%组的对虾成活率最高(96.67%),w(淀粉)=25%组最低(66.67%);w(淀粉)在25%~35%时,对虾的增质量率、成活率、SGR显著低于w(淀粉)为10%、15%和20%组(P<0.05).w(淀粉)=20%组的体蛋白含量最低(72.24%),w(淀粉)=30%组最高(75.27%),其余各组没有显著性差异;高淀粉组体脂肪含量相对较高.从凡纳滨对虾的肝胰脏组织学切片观察到,饲料w(淀粉)为10%~35%的范围内,肝胰脏脂肪无异常积累.肝胰腺中脂肪合成酶活性很低,苹果酸脱氢酶活性随饲料淀粉含量的增加而升高.总之,在饲料蛋白含量为w=38%左右时,凡纳滨对虾饲料适宜的淀粉含量(w)为10%~20%.%The ability of Litopenaeus vannamei (initial mean weight: (0. 96 ±0. 02) g) to utilize different levels of cornstarch was examined in terms of growth indices, body composition, microscopic structure and lipogenic enzyme of the hapetopancreas. Six isonitrogenous semipurified diets were fed to shrimp to satiation for 8 weeks in triplicate tanks (30 shrimps per tank) connected to a natural brackish water ( salinity: 6‰ ~ 14‰) recirculating system. Diets contained different levels of cornstarch (w, 10%、 15%、20%、 25%、 30%、 35% ) as the source of carbohydrate. Weight gain (WG), survival rate and SGR were considerably affected by cornstarch

  12. Isolation and Preliminary Identification of Five Suspicious Pathogenic Bacteria from Aquatic Water of White-leg Shrimp Litopenaeus vannamei%南美白对虾养殖水体5株疑似病原菌的分离与初步鉴定

    Institute of Scientific and Technical Information of China (English)

    韩丽; 张玉婷; 孙晓红; 戴习林; 潘迎捷; 赵勇

    2008-01-01

    应用TCBS(硫柠胆蔗琼脂)培养基从上海金山区南美白对虾(Litopenaeus vannamei)养殖水体中分离筛选出5株优势疑似病原菌(编号为S1、S2、S3、S4、S5),应用ARDRA图谱分析、ERIC-PCR分析结合16S rDNA的PCR扩增与测序等多相分子生物学方法对其进行了鉴定.结果表明,S1为Pseudomonas属细菌;S2为Leclercia属细菌;S3为Exihuobacterium属细菌;S4为Jeotgalibacillus属细菌,S5为Staphylococcus属细菌.GenBank登录号为EU251039~EU251043.其中S2、S3、S4是首次从南美白对虾养殖水体中分离出来的疑似病原菌.结果还表明,TCBS培养基并非专一性选择培养弧菌的培养基,其他疑似病原菌也可以在该培养基中生长.

  13. Résistance de la crevette Litopenaeus stylirostris à la bactérie pathogène Vibrio penaeicida : Physiologie, immunologie et pathologie comparées d’une population sélectionnée sur un critère de survie aux épisodes de mortalité et d’une population témoin non sélectionnée.

    OpenAIRE

    De Decker, Sophie

    2004-01-01

    La filière crevette de Nouvelle-Calédonie reposesur la maîtrise de la reproduction contrôlée de la crevette Litopenaeus stylirostris, espèce introduite dans les années 1980. La difficulté majeure que rencontre la filière depuis une dizaine d’années est la récurrence du « Syndrome 93 », qui s’exprime sous forme d’épisodes de mortalités lors des baisses de température aux intersaisons. Ces mortalités sont associées à la bactérie pathogène Vibrio penaeicida Une expérience de sélection sur un cri...

  14. Peumus boldus (Monimiaceae, Magnoliopsida) una especie silvestre promisoria de Chile

    OpenAIRE

    San Martín Acebedo, José; Doll, Ursula

    1998-01-01

    Se organiza y sintetiza la información relacionada con la botánica, ecología y el contenido químico de extraíbles de hojas, corteza y madera de Peumus boldus (Monimiaceae, Magnoliopsida), árbol siempreverde, autóctono, endémico y termófilo de los bosques y matorrales esclerófilos naturales del área mediterránea de Chile. La popularidad y conocimiento de la especie tiene arraigo en la medicina popular empírica, dado el efecto farmacológico atribuible fundamentalmente, al alcaloide boldina, pre...

  15. ANÁLISES COPROPARASITOLÓGICAS DE AVES SILVESTRES CATIVAS

    Directory of Open Access Journals (Sweden)

    Alessandra Snak

    2014-12-01

    Full Text Available In most instances of parasitism in captive birds there is no expression of clinical signs. This fact underscores the importance of performing frequent parasitological examinations in these animals. The aim of this study was to do a coproparasitological analysis to monitor parasitic infections in captive birds in Danilo Galafassi Municipal Park (Municipal Zoo of Cascavel-PR, project approved by the Committee of Ethics and Animal Welfare (CEBEA Campus Palotina-UFPR, protocol nº 29/2010. A total of 228 fecal samples were analyzed from 37 species of captive birds from 22 enclosures in the period from August 2010 to July 2012. Chilled feces were analyzed through flotation methods of Willis-Mollay and simple sedimentation of Hoffmann, Pons and Janer. Out of the analyzed samples, 127 (55.7% were positive and among these 55 (43.3% presented mixed infection. The parasites that were found belonged to the genus Strongyloides, Eimeria, Capillaria, Deletrocephalus and Isospora, the superfamilies Strongyloidea, Ascaroidea and Spiruroidea, the order Trichurida and the class Cestoda. The examinations results of the enclosure of Jabiru mycteia (tuiuiú and Cariama cristata (seriema were negative throughout the study period. Hygienic-sanitary measures and movement control measures of animals and people circulation were introduced to minimize bird’s infection, but it requires further analysis to assess their impact.

  16. Bioseguridad en el Manejo de Fauna Silvestre y no Convencional

    OpenAIRE

    Nestor Varela

    2011-01-01

    La bioseguridad es un conjunto de actuaciones para prevenir la pérdida de la integridad biológica a diversas escalas, estando relacionada como concepto a múltiples campos y disciplinas. En las ciencias de la salud, la bioseguridad hace referencia a la prevención de la contaminación derivada del contacto con órganos o tejidos de origen biológico y microorganismos. Los problemas traumáticos, las reacciones alérgicas o irritativas y las zoonosis son las causas de enfermedad más frecuentes en las...

  17. Efeito de extratos de plantas silvestres da família Solanaceae sobre o controle de Brevicoryne brassicae em couve (Brassica oleracea var. acephala Extracts effect of wild plants of the Solanaceae family on Brevicoryne brassicae control in cabbage (Brassica oleracea var. acephala

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    Patrícia Braga Lovatto

    2004-08-01

    Full Text Available O objetivo principal deste trabalho foi testar o efeito de extratos de plantas silvestres da família Solanaceae disponíveis na região do Vale do Rio Pardo, RS, sobre o pulgão-da-couve (Brevicoryne brassicae, praga agrícola de significativa importância. Nove espécies tóxicas de Solanaceae estão disponíveis na região de estudo: seis pertencem ao gênero Solanum, uma ao gênero Brugmansia e duas são cultivadas e pertencem ao gênero Nicotiana e Capsicum. A fim de determinar a capacidade de repelência e o efeito inseticida, foram testadas as seguintes espécies: B. suaveolens (trombeteira, C. annuum var. variegated (pimenta-de-jardim, N. tabacum var. virginia (fumo, S. aculeatissimum (joá-bravo, S. americanum (erva-moura, S. diflorum (tomatinho, S. fastigiatum var. acicularium (jurubeba, S. fastigiatum var. fastigiatum (jurubeba e S. sisymbriifolium (arrebenta-cavalo. Para obtenção dos extratos foram utilizadas folhas, flores e frutos aplicando-se duas técnicas distintas de elaboração: decocção do material fresco e extração a frio do material seco. Quanto à capacidade de repelência dos extratos, os tratamentos utilizando material fresco de S. fastigiatum var. acicularium (frutos verdes e maduros, 2,5% e 5% de concentração e S. diflorum (frutos verdes e maduros, 2,5% e 5% de concentração demonstraram maior eficácia. Nos testes sobre a biologia do inseto os tratamentos mais eficazes como inseticida foram S. fastigiatum var. fastigiatum e var. acicularium (folhas a 10% de concentração.The objective of this study was to test the effect of plant extracts from Solanaceae available in Vale do Rio Pardo region, RS, on the aphid Brevicoryne brassicae, agricultural pest of significant importance. Nine toxic Solanaceae species are available in the area of study: six are wild belonging to the genus Solanum, one to the genus Brugmansia, and two are cultivated and belong to the genus Nicotiana and Capsicum. To determine the

  18. Variabilidade genética de acessos obtidos de populações cultivadas e silvestres de maracujazeiro-doce com base em marcadores rapd Genetic diversity obtained from cultivated population and native accesses of seewt passion fruit based on rapd markers

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    Graciele Bellon

    2009-03-01

    Full Text Available O maracujazeiro-doce (Passiflora alata Curtis, devido a preços diferenciados, vem ganhando importância dentro do mercado de frutas in natura. O melhoramento genético é fundamental para elevar a qualidade e a produtividade da cultura. Os marcadores moleculares do DNA têm sido muito úteis por permitirem a obtenção de um número praticamente ilimitado de polimorfismo genético sem influência do ambiente. Objetivou-se, neste trabalho, estudar a variabilidade genética de 17 acessos de maracujá-doce, com base em marcadores moleculares RAPD. Um acesso de P. quadrangularis e um de P. edulis foram utilizados como outgroups. Amostras de DNA genômico de cada acesso foram extraídas e 11 iniciadores decâmeros (OPD 04; 07; 08 e16; OPE 18 e 20; OPF 01 e 14; OPG 08; OPH 12 e 16 foram utilizados para a obtenção dos marcadores. Os marcadores obtidos foram convertidos em uma matriz de dados binários, a partir da qual foram estimadas as distâncias genéticas entre os acessos e realizadas análises de agrupamento e de dispersão gráfica. Do total de marcadores, considerando-se apenas os acessos de P. alata, observaram-se 87 (62,12% bandas polimórficas, evidenciando a grande variabilidade intraespecífica. A análise de agrupamento realizada com base nas distâncias genéticas permitiu subdividir os 17 acessos de P. alata em, pelo menos, cinco grupos de similaridade genética. Os acessos silvestres foram os que mais contribuíram para a ampliação da base genética dos materiais estudados, abrindo perspectivas para o uso desses materiais em programas de melhoramento.Sweet passion fruit (Passiflora alata Curtis is gaining importance in the in natura fruit market due to differential value. Genetic breeding is crucial to improve crop quality and productivity. Molecular markers of DNA have been very useful by allowing obtaining a virtually unlimited number of genetic polymorphism without environment influence. This work's objective was to study the

  19. Parámetros hematológicos de la comadreja overa, Didelphis albiventris (Lund, 1841, de poblaciones silvestres del centro de la Argentina Haematological parameters of the White-eared Opossum, Didelphis albiventris (Lund, 1841, wild populations of central Argentina

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    E.L. Tarragona

    2011-12-01

    Full Text Available El objetivo de este trabajo fue obtener parámetros hematológicos en poblaciones silvestres de comadreja overa Didelphis albiventris de la región centro de la Argentina. Se capturaron 39 individuos, a todos ellos se les realizó un recuento total y diferencial de células sanguíneas. Los valores promedio (± desvío estándar obtenidos fueron: eritrocitos 5,01 (±2,4 × 10(6µL-1; leucocitos 27.097 (±14.313 µL-1; neutrófilos 7.600 (±7.097 µL-1 (incluye inmaduros, 794 (±2.738 µL-1; linfocitos 13.110 (±7.541 µL-1; basófilos 1.218 (±1.441 µL-1; eosinófilos 1.983 (±2.458 µL-1; y monocitos 2.321 (±2.818 µL-1. La existencia de asociaciones entre estos parámetros y factores ambientales (estación y del animal (sexo y edad fue evaluada mediante regresión lineal multivariable. Se estableció que los neutrófilos estuvieron significativamente asociados a sexo (p=0.006. Los machos tenían la mitad de los niveles de neutrófilos que las hembras. Los eosinófilos estuvieron asociados a la estación. En verano hubo significativamente más eosinófilos que en invierno (p=0.042. En los extendidos sanguíneos se observaron alteraciones morfológicas de eritrocitos en aproximadamente 55% de las muestras analizadas, las más habituales fueron pilas en monedas y estomatocitos. También se visualizó un tipo celular no descripto anteriormente para esta especie, leucocito anular.The objective of this study was to assess haematological parameters in free-ranging white-bellied opossums (Didelphis albiventris in the central region of Argentina. Total and differential blood cell counts were conducted in thirty-nine captured individuals. The mean (± standard deviation values obtained were: erythrocytes 5.01 (±2.4 x 106µL-1, leukocytes: 27,097 (±14,313 µL-¹; neutrophils 7,600 (±7,097 µL-1 (including immature neutrophils: 794 (±2,738 µL-1; lymphocytes: 13,110 (±7,541 µL-1; basophils: 1,218 (±1,441 µL-1; eosinophils: 1,983 (±2,458 µL-1

  20. Evaluación de poblaciones segregantes producidas a partir de cruzamientos entre tomate cultivado y la accesión silvestre PI134418 de Solanum habrochaites var. glabratum resistente al pasador del fruto. Evaluation of segregating populations from crosses between cultivated tomato and PI134418 wild accession of Solanum habrochaites var. glabratum with resistance to the fruit borer.

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    Edwin F. Restrepo S.

    2008-01-01

    Full Text Available Se realizó la evaluación de poblaciones segregantes producidas a partir de cruzamientos entre tomate S. lycopersicum L. cv. maravilla y la accesión silvestre PI134418 de S. habrochaites var. glabratum resistente al pasador del fruto N. elegantalis. Se encontró que hay dominancia de los alelos que disminuyen la expresión del caraacute;cter peso promedio de fruto. El método del retrocruzamiento resultó maacute;s eficiente que el de la autofecundación, para contrarrestar la dominancia de los alelos que codifican por el tamaño pequeño del fruto. Los tricomas y el número promedio de frutos por racimo no ejercieron ningún efecto sobre el ataque de los frutos por parte del pasador; mientras que el peso promedio de fruto sí tuvo efecto importante sobre dicho ataque, de tal manera que el insecto tiende a producir mayor daño a medida que se incrementa el peso promedio de fruto. Las poblaciones RC1 y RC2 se comportaron como las típicas segregantes para los caracteres evaluados y exhibieron amplia variabilidad en los mismos. El método del retrocruzamiento fue efectivo para romper la asociación negativa entre el peso promedio de fruto y la resistencia al insecto. El peso promedio de algunos de los frutos en los recombinantes RC2 fluctuó entre 45.1 y 68.6 g y mostraron resistencia al pasador.Evaluation of segregating populations from crosses between cultivated tomato S. lycopersicum L. cv. maravilla and PI134418 wild accession of Solanum habrochaites var. glabratum with resistance to the fruit borer N. elegantalis was made. There was dominance of alleles that decrease the expression of the character mean fruit weight. The backcrossing method was more efficient than selfing in order to counteract the dominance of alleles that encode by small fruit size. The thrichomes and the mean number of fruit per bunch did not produce any effect on the fruit attack by the fruit borer; while that mean fruit weight had important effect on this attack

  1. Nucleotide Mixture Supplementation Affects Non-specific Immune and Antioxidant Indices of Juvenile Litopenaeus vannamei%饲料中添加核苷酸混合物对凡纳滨对虾幼虾非特异性免疫和抗氧化指标的影响

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    许丹丹; 黄燕华; 曹俊明; 蓝汉冰; 王国霞; 张荣斌; 陈晓瑛; 严晶

    2011-01-01

    本试验旨在研究外源核苷酸混合物对凡纳滨对虾(Litopenaeus vannamei)幼虾非特异性免疫和抗氧化指标的影响.选取960尾初始体重为(0.43±0.01)g的凡纳滨对虾,随机分为8组(每组设3个重复,每个重复40尾虾),分别投喂在基础饲料中添加0(对照)、0.1、0.2、0.4、0.6、0.8、1.0和1.2g/kg核苷酸混合物(5'-腺苷酸:5'-胞苷酸:5'-尿苷酸二钠:5'-肌苷酸二钠:5'-鸟苷酸二钠=1:1:1:1:1,质量比)的试验饲料,养殖期为5周.结果表明,饲料中添加0.4~1.2g/kg核苷酸混合物可显著或极显著提高凡纳滨对虾幼虾血细胞总数(THC)(P<0.05或P<0.01).与对照组相比,0.6和0.8/kg组血清超氧化物歧化酶(SOD)活性显著升高(P<0.05).0.4g/kg组血清总抗氧化能力(T-AOC)与对照组相比提高了51.0%(P>0.05),并显著高于1.2g/kg组(P<0.05).各组肝胰腺SOD活性和T-AOC随饲料中核苷酸混合物添加量的增加先升高后降低,均在0.4g/kg组达到最高值.各组血清和肝胰腺过氧化物酶(POD)和碱性磷酸酶(AKP)活性均无显著差异(P>0.05).饲料中添加0.2~1.2g/kg核苷酸混合物可显著降低对虾肝胰腺丙二醛(MDA)含量(P<0.05),而各组血清MDA含量差异不显著(P>0.05).0.2、0.4和0.6g/kg组肌肉抗超氧阴离子自由基(O2-·)活性显著高于1.0和1.2g/kg组(P<0.05),其他各组间差异不显著(P>0.05).结果提示,饲料中添加一定量的核苷酸混合物能提高凡纳滨对虾幼虾的非特异性免疫和抗氧化能力,建议添加量为0.4~0.6g/kg.%A 5-week feeding trial was conducted to study the effects of dietary nucleotides on non-specific immune and antioxidant indices of juvenile Litopenaeus vannamei. A total of 960 shrimp with an average body weight of (0.43 ±0.01) g were randomly allocated into 8 groups with 3 replicates per group and 60 shrimp per replicate. The control group were fed with the basal diet, while the other 7 groups were fed with the basal diets added

  2. An Experimental Study on Optimization for Polyculture of Portunus trituberculatus with Litopenaeus vannamei, Ruditapes philip pinarum and Gracilaria lichevoides%三疣梭子蟹、凡纳滨对虾、菲律宾蛤仔和江蓠混养结构优化的实验研究

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    班文波; 田相利; 董双林; 张凯; 高明亮; 张东旭; 奉杰; 张庆起

    2015-01-01

    The study was designed and conducted to optimize the structure of polyculture for Portunus trituberculatus ,Litopenaeus vannamei ,Ruditapes philippinarum and Gracilaria lichevoides ,and to compare their water quality parameters .Crabs ,shrimps and clams were mixed with Gracilaria lichev‐oides ,six treatments were designed respectively was C ,CS ,CSB ,CSBJ1 ,CSBJ2 ,CSBJ3 (C :crab ;S :shrimp;B :clam ;J :Gracilaria lichevoides) .The result showed that the individual specifications of crabs in different treatments were not significantly different(P>0 .05);The highest yield of crab were 3255 .24 kg/hm2 for the treatment of CSBJ2 ;The body weight ,sgr and survival rate of CSBJ1 and CS‐BJ2 were significantly higher than other treatments(P0 .05);During the period of culturing ,there were significantly lower NO2 -N in CSBJ2 than in other treatments on the whole(P<0 .05);The treatments which mixed with Gracilaria lichevoides could significantly reduce the concentration of ammonia nitrogen(P< 0 .05) . Total nitrogen and total phosphorus concentration of CSBJ1 and CSBJ2 were significantly lower than other treatments in the middle and late cultivation(P<0 .05) .In this study , Portunus trituberculatus system mixed with Gracilaria lichevoides in suitable density could improve water quality ,reduce the concentration of total nitrogen ,total phosphorus and ammonia nitrogen ,nitrate nitrogen and improve the production and survival rate of Portunus trituberculatus and Litopenaeus vannamei .Under the condition of this research ,the treatment giving the best culturing benefit was crabs at 6 ind/m2 , shrimps at 45 ind/m2 and clams at 45 ind/m2 ,Gracilaria lichevoides at 0 .36 kg/m2 .%利用海水陆基实验围隔优化了三疣梭子蟹(Portunus trituberculatus)、凡纳滨对虾(Litopenaeus van‐namei)、菲律宾蛤仔(Ruditapes philippinarum)和菊花江蓠(Gracilaria lichevoides)的混养结构。实验设6个处理组,分别为蟹-虾(CS)、蟹-虾

  3. 几株肠道益生菌对凡纳滨对虾非特异免疫力和抗病力的影响%Effects of probiotics from the shrimp intestine on the non-specific immunity and antiviral capacity of Litopenaeus vannamei

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    李桂英; 宋晓玲; 孙艳; 麦康森; 谢国驷; 黄倢

    2011-01-01

    We evaluated the effects of probiotics on the non-specific immunity and antiviral capacity of Lito-penaeus vannamei. We fed groups of shrimp (body weight 3.2 g±0.26 g) a diet supplemented with either l.0×108 CFU/g Bacillus firmus (live) plus 1 % Photobacterium damsela (inactivated), 1 .0× 108 CFU/g Bacillus firmus (live), or l.0×108 CFU/g Bacillus firmus (live) plus 1% Vibrio alginolyticus (inactivated). The control groups were fed a non-supplemented diet. Each diet was fed to triplicate groups of 60 shrimp that were reared in aquaria. We randomly selected 10 shrimp per group every 5 d and measured a range of humoral immune parameters (superoxide dismutase SOD, acidic phosphatase ACP, alkaline phosphatase AKP, nitric oxide synthase NOS and lysozyme UL) in each individual. The shrimp were challenged with white spot syndrome virus (WSSV) after two weeks. We then calculated the cumulative mortality of each experimental group. Supplementation with bacteria significantly increased serum SOD, ACP, AKP, and NOS compared with the controls. There was significantly lower cumulative mortality in shrimp that were fed the probiotics diet (10.7%, 39.3% and 26.2%, respectively) compared to the control (64.28%). Our results suggest that supplementing the diet with shrimp intestine probiotics improves immunity and the antiviral capacity of Litopenaeus vannamei.%以基础饲料为对照组,在基础饲料中分别添加坚强芽孢杆菌活菌(Bacillus firmus)、坚强芽孢杆菌活菌(1.0×108 CFU/g)+美人鱼发光杆菌(Photobacterium damsela)灭活菌(1%)、坚强芽孢杆菌活菌(1.0×108 CFU/g)+溶藻弧菌(Vibrio alginolyticus)灭活菌(1%)配制3种免疫饲料.每组3个重复,对个体质量为(3.2±0.26)g的凡纳滨对虾(Litopenaeus vannamei)进行了为期30d的养殖实验.每5d取样,以血清中的酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、一氧化氮合酶(NOS)、超氧化物歧化酶(SOD)和溶菌酶(UL)活性为免疫指标,探讨了肠道益生

  4. Contenido de alimento y metabolismo ceco-cólico en el tracto digestivo de poblaciones silvestres de iguana negra (Ctenosaura pectinata en Morelos, México Cecum-colic content of food and metabolism in the digestive tract of wild population black lizard (Ctenosaura pectinata in Morelos, Mexico

    Directory of Open Access Journals (Sweden)

    L Vélez-Hernández

    2012-01-01

    Full Text Available Se colectaron cinco tractos digestivos de iguana negra (Ctenosaura pectinata en estado silvestre en el estado de Morelos, México, para conocer la composición botánica de la dieta y el metabolismo ceco-cólico. Se observaron frotis realizados del contenido ceco-cólico para registrar la presencia de protozoarios, bacterias y parásitos intestinales existentes. Se obtuvo la media y desviación estándar de las características evaluadas. En el contenido ceco-cólico se identificaron las especies vegetales guamúchil (Phitecellobium dulce y huizache (Acacia farnesiana. Se estimó en base a materia seca la porción vegetal que conforma la dieta, la cual se distribuyó en frutos (62,9%, hojas (30,6%, rebrotes (2,9% y flores (3,5%. La región ceco-cólica se caracterizó por la concentración (mmol de los ácidos grasos acético (68,9 ± 10,6, butírico (11,98 ± 5,7, propiónico (9,04 ± 1,5 y total (89,9 ± 15, con pH de 7,45 y concentración de nitrógeno amoniacal (7,4 ± 2,8 mg/dl. La microbiota que se observó fue el protozoario Nyctotherus spp. Las bacterias totales oscilaron en concentraciones 7 x 10 a 9 x 10/g, siendo las celulolíticas de 9,2 x 10 hasta 3,5 x 10/g de contenido cecal. Los parásitos encontrados son nematodos identificados como miembros de la superfamilia Oxyuroidea con 655 ± 265/g huevos y 6,300 ± 329 adultos/iguana. Se concluye que la fermentación de la región ceco-cólica de la iguana negra en estado adulto se comporta de manera similar como en las especies herbívoras, en las cuales la fermentación se manifiesta de acuerdo con el alimento consumido. La concentración dietaria de proteína cruda fue de 14,5% y energía de 2,193 Mcal/kg y son animales parasitados con oxiuros.Five digestive tracts of black lizards (Ctenosaura pectinata from a wild population were collected in Morelos State, México, in order to identify the plant composition in the diet and the cecum-colic metabolism. Smears obtained from cecum

  5. 不同形式蛋氨酸对凡纳滨对虾肝胰腺蛋氨酸腺苷转移酶活性的影响%Effect of different methionine on methionine adenosyl transferase activities of hepatopancreas of Pacific white shrimp (Litopenaeus vannamei)

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    迟淑艳; 谭北平; 杨奇慧; 董晓慧; 刘泓宇

    2012-01-01

    试验在低鱼粉饲料中添加不同形式蛋氨酸[晶体蛋氨酸(MET)、邻苯二甲酸醋酸纤维素包被蛋氨酸(CAP)、棕榈酸甘油酯包被蛋氨酸(TPA)、树脂包被蛋氨酸(RES)和羟基蛋氨酸(MHA)],评价对凡纳滨对虾肝胰腺蛋氨酸腺苷转移酶活性的影响.配制5种等氮、等能饲料饲喂凡纳滨对虾,以晶体蛋氨酸为对照组进行50 d的养殖试验.分别在第28 d和50 d时,取对虾肝胰腺测定蛋氨酸腺苷转移酶活性.结果表明,微胶囊化对晶体蛋氨酸在肠道有一定的缓释作用,可以改善晶体蛋氨酸和蛋白质结合态蛋氨酸的吸收效率.%This study was conducted to evaluate the effects of supplementation of crystalline methionine or coated methionine in low fishmeal diets of Pacific white shrimp (Litopenaeus vannamei) on methionine adenosyl transferase activities (MAT). Five iso- nitrogen and iso- caloric diets were prepared in which diets were supplemented with cellulose- acetate- phthalate (CAP), tripalmitin-polyvinyl alcohol (TPA), acrylic resin(RES) coated L-methionine, hydroxyl-melhionine calcium (MHA) and crystalline L-methionine diet (MET, control), respectively. Hepatopancreas was taken for determination of MAT activity at 28 d and 50 d, respectively. The results indicated that the microencapsulation was the better way to delay the release rate for crystalline methionine in the intestine of shrimp and could improve the absorption efficiency of the crystalline amino acids and protein-bound amino acids.

  6. 氨氮胁迫下凡纳滨对虾对副溶血弧菌的易感性%The Immune Response of Litopenaeus vannamei and Its Susceptibility to Vibrio parahaemolyticus Under Stress Caused by Ammonia Nitrogen at Different Concentrations

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    葛红星; 李健; 陈萍; 梁忠秀; 任海; 李东利

    2014-01-01

    Acute Hepatopancreatic Necrosis Syndrome (AHPNS), also know as Early Mortality Syndrome (EMS), was officially reported in China in 2010 and has caused large-scale die-offs of cultivated shrimp in several Asian countries. A research team of the University of Arizona has identified that the pathogen is Vibrio parahaemolyticus which is a strain of a bacterium commonly found in brackish coastal waters around the globe. Because V. parahaemolyticus is a conditioned pathogen, it is very important to study the effects of ammonia nitrogen on the toxicity of this pathogen to Litopenaeus vannamei. To evaluate the effects of total ammonia nitrogen during the outbreak of AHPNS in L. vannamei, we examined the susceptibility to the pathogen, the non-specific immunity and the expression of LvLT mRNA in the hepatopancreas of the shrimp. Shrimp underwent the ammonia stress for 20 days with different ammonia nitrogen concentrations:2.5 mg/L, 5.0 mg/L, 7.5 mg/L, 10.0 mg/L and the control concentration. After the exposure to V. parahaemolyticus, the death rate of shrimp perked in 6–24 h. The accumulative mortality rates of the treated groups were 0, 8%, 12%, 20%and 36%respectively at 120 h. The activities of phenoloxidase (PO) reached the lowest at 12 h in the control, 2.5 and 5.0 mg/L groups, and at 24 h in the 7.5 and 10.0 mg/L groups. The activity of superoxide dismutase (SOD) increased at first and then gradually declined, and lysozyme (LSZ) exhibited the same trend. The expression of LvLT mRNA in the hepatopancreas increased at 6 h in all groups and was significantly higher in the control, 2.5, 5.0 and 7.5 mg/L group than that in the 10.0 mg/L group. The LvLT mRNA expression reached the maximum at 12 h and then declined gradually at 24 h. The results indicated that high ammonia nitrogen could cause depression in the immunity of L. vannamei, and subsequently increase their susceptibility to V. parahaemolyticu and the resultant mortality rate. Therefore it is crucial to regulate

  7. 凡纳滨对虾半集约化养殖池塘浮游微藻优势种变动规律及其对养殖环境的影响%Change of dominant species of planktonic microalgae in Litopenaeus vannamei' s semi -intensive culture ponds and its impact on the culture environment

    Institute of Scientific and Technical Information of China (English)

    彭聪聪; 李卓佳; 曹煜成; 刘孝竹; 胡晓娟

    2011-01-01

    Investigations on the microalgae community were conducted in Litopenaeus vannamei' s semi-intensive culture ponds.The variation about dominant species of microalgae in shrimp ponds were identified ,moreover,their relationships with culture environment and culture efficiency were analysised.The results showed that a total of 16 dominant species of microalgae were identified.The dominant species in initial phase were Chlorella pyrenoidosa, Nitzschia closteriu and Peridinium pusillum; the ones in the mid-phase were Cyclotella operculata, Lyngbya gardnari, Oscillatoria chlorine, K.microscopica Nygaard, C.kessleri, Cyli