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Sample records for calliphoridae criada emdieta

  1. Desenvolvimento pós-embrionário de Cochliomyia macellaria (Fabricius (Diptera: Calliphoridae, criada em duas dietas naturais, sob condições controladas

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    Débora Cardoso

    2012-08-01

    Cochliomyia macellaria tem interese médico e médico veterinário por ser veiculadora de enteropatógenos e causadora de miíase secundária. A busca por dietas com baixo custo que mantenham a qualidade da criação torna-se necessária. Objetivou-se avaliar a dieta moela de frango no desenvolvimento pós-embrionário de C. macellaria, comparada a carne bovina. Quarenta neolarvas (3ª geração foram transferidas para 80g de dieta (quatro repetições/tratamento. Foi registrada a massa corporal das larvas maduras, e estas transferidas para tubos de ensaio e mantidas a T 30ºC dia/28ºC noite, UR 70±10% e 12h de fotofase. As observações foram diárias. Houve diferença significativa entre a massa corporal das larvas criadas na dieta moela de frango (0,067g e carne bovina (0,062g. Não houve diferença significativa entre a duração média em dias do estágio larval (4,00; 4,17, pupal (4,09; 4,04; de neolarva a adulto (8,07 e 8,16, para moela e carne, respectivamente. A viabilidade média larval, pupal e total (> 85% não diferiram estatisticamente pelo teste t de Student, 5% de significância. Não houve desvio da razão sexual e não foi observada anormalidade dos adultos. A dieta moela de frango é uma alternativa eficaz e de baixo custo para criação de C. macellaria em laboratório.

  2. Development Period of Forensic Importance Calliphoridae (Diptera: Brachycera in Urban Area Under Natural Conditions in Manaus, Amazonas, Brazil

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    Alex Barros-Souza

    2012-06-01

    Resumo. Para descrever o tempo de desenvolvimento dos imaturos de Calliphoridae sob condições naturais, dois experimentos foram realizados no Campus II do Instituto Nacional de Pesquisas da Amazônia (INPA, Manaus, Amazonas, um na estação chuvosa e o outro na estação menos chuvosa. Cadáveres de porcos domésticos (25kg cada foram utilizados como substrato atrativo para a ovipostura dos califorídeos. Fêmeas grávidas de Calliphoridae foram coletadas e os ovos foram transferidos para potes plásticos contendo placas de Petri com carne bovina moída. As espécies criadas, com respectivo tempo de desenvolvimento de ovo a adulto (em dias, na estação mais chuvosa e menos chuvosa, foram: Chrysomya albiceps (Wiedemann, 14,5 e 9,4 dias, Chrysomya megacephala (Fabricius, 10,7 e 9,4, Hemilucilia segmentaria (Fabricius, 11,5 e 10,7 Lucilia eximia (Wiedemann 19,4 e 14,3 e Paralucilia paraensis (Mello, 11,8 dias, essa criada somente na estação menos chuvosa. Este é o primeiro registro do tempo de desenvolvimento de P. paraensis.

  3. Rearing five species of Diptera (Calliphoridae of forensic importance in Colombia in semicontrolled field conditions

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    María C. Vélez

    2008-01-01

    Full Text Available The family Calliphoridae is widely known to lead the colonization of corpses and their development rates are frequently used to estimate the postmortem interval. This study presents the larval growth of five forensically important species of Calliphoridae in Colombia. Rearing took place in semicontrolled field conditions where the egg masses were collected. We show curves of larval growth, larval length and time intervals to reach all immature stages for Lucilia eximia and Cochliomyia macellaria at two sites with different climatic conditions and for Chrysomya albiceps, Chrysomya megacephala and Calliphora nigribasis at one site. Overall, high temperatures speeded up the development of the species reared at two different sites, whereas low temperatures for C. nigribasis, lengthened the total development time. Differences between this study and others can be explained by the experimental conditions in the field without the possibility of strict laboratory rearing controls.La familia Calliphoridae es ampliamente conocida por liderar la colonización de los cadáveres y sus tasas de desarrollo son frecuentemente utilizadas para estimar el intervalo postmortem. Este estudio presenta el crecimiento larval de cinco especies de Calliphoridae de importancia forense en Colombia, considerando que la cría se dio en condiciones de campo semicontroladas en los lugares donde las masas de huevos fueron colectadas. Mostramos también, los intervalos de longitud y el tiempo empleado en alcanzar todos los estadios inmaduros para Lucilia eximia y Cochliomyia macellaria en dos lugares con diferentes condiciones climáticas y Chrysomya albiceps, Chrysomya megacephala y Calliphora nigribasis, en un solo lugar. En general, las altas temperaturas producen una aceleración en el desarrollo de las especies criadas en dos sitios diferentes, mientras que bajas temperaturas para C. nigribasis, alargaron el tiempo utilizado para completar el desarrollo. Las diferencias

  4. [Biological characteristics of calliphoridae and its application in forensic medicine].

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    Zhao, Boa; Wen, Charn; Qi, Li-Li; Wang, He; Wang, Ji

    2013-12-01

    Diptera Calliphoridae is the first major kind of flies that appears on the decomposed corpses. In forensic entomology, according to the living characteristics of Calliphoridae flies, we could accurately estimate postmortem interval (PMI) in a murder or unidentified case and could provide useful clues to solve the case. This paper introduces the characteristics of the biology and morphology of Diptera Calliphoridae, and reviews the combined application of forensic entomology, molecular biology, mathematical morphology and toxicology.

  5. Comportamento de poedeiras criadas a diferentes densidades e tamanhos de grupo em ambiente enriquecido

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    Danilo Florentino Pereira

    2013-06-01

    Full Text Available O objetivo deste trabalho foi determinar diferenças comportamentais entre poedeiras criadas sob diferentes densidades e tamanhos de grupo, em condições de ambiente enriquecido. Foram utilizadas poedeiras Isa Brown com idade entre 30 e 32 semanas alojadas em galpões de escala reduzida e distorcida. As aves foram criadas durante 28 dias, em baias com cama de maravalha, poleiro e ninho. Foram avaliados dois tamanhos de grupos (6 e 12 aves e duas densidades de criação (774 e 1.440 cm² por ave, em arranjo fatorial com três repetições. Em amostras de vídeo de 15 min, foram registrados as frequências e os tempos de expressão dos comportamentos: arrumar penas, banho de areia, bater asas, beber água, bicar, coçar a cabeça, ciscar, comer, empoleirar, esticar perna, perseguir, sentar e visitar o ninho. Foram observados efeitos significativos dos tratamentos e da interação entre eles. O grupo de seis aves manifestou aumento da frequência de comportamentos que indicam maior frustração das aves, independentemente da densidade. O tamanho de grupo é o fator mais importante para o bem-estar das aves.

  6. Flies (Calliphoridae, Muscidae and Beetles (Silphidae from Human Cadavers in Cali, Colombia

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    Barreto Mauricio

    2002-01-01

    Full Text Available Adult specimens of Cochliomyia macellaria, Chrysomya megacephala, Ch. rufifacies, Lucilia sp. (Calliphoridae, Musca domestica (Muscidae, Oxelytrum discicolle (Silphidae and Sarcophagidae were recovered from 12 human cadavers in Cali, Valle, Colombia. Information regarding these findings is presented.

  7. Desempenho e qualidade de ovos de galinhas poedeiras criadas em gaiolas enriquecidas e ambiente controlado

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    Daniele L. de Oliveira

    2014-11-01

    Full Text Available Objetivou-se, neste trabalho, avaliar o desempenho e a qualidade dos ovos de poedeiras criadas em gaiolas enriquecidas e ambiente controlado, alojadas em câmara climática sob três temperaturas: 20, 26 e 32 ºC e U.R, 60%. As gaiolas apresentavam bebedouros, comedouros, ninhos e uma área com areia e lixa. Foram utilizadas 36 poedeiras da linhagem Dekalb White, com 27 semanas de idade, com seis aves/gaiolas. Foram analisados o consumo de ração e água, a produção e a qualidade dos ovos. O delineamento experimental foi inteiramente casualizado, com três tratamentos e seis repetições. Nas condições ambiente de 20 e 26 ºC as aves se mostraram dentro da zona de conforto térmico sem diferença significativa e apresentando efeito positivo na qualidade dos ovos com melhores valores das médias nos parâmetros de qualidade. Na temperatura ambiente de 32 ºC, considerada fora da zona de conforto térmico, as aves apresentaram evidências de estresse térmico, propiciando aumento da ingestão de água, redução no consumo de ração, redução nos valores de percentual de produção e nos parâmetros de qualidade dos ovos. As gaiolas enriquecidas foram adequadas às galinhas apresentando produção de ovos com boa resistência da casca.

  8. Action of Ants on Vertebrate Carcasses and Blow Flies (Calliphoridae).

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    Paula, Michele C; Morishita, Gustavo M; Cavarson, Carolina H; Gonçalves, Cristiano R; Tavares, Paulo R A; Mendonça, Angélica; Súarez, Yzel R; Antonialli-Junior, William F

    2016-11-01

    Forensic entomology is a science that uses insect fauna as a tool to assist in criminal investigations and civil proceedings. Although the most researched insects are the Diptera and Coleoptera, ants may be present in all stages of decomposition. The aim of this study was to evaluate the role of ants and their action on blow flies during the decomposition process. Experiments were performed in which four pig carcasses were exposed in the cold and dry season (November/2012 and March/2013) and four in the hot and wet season (May/2013 and August/2013). Flies were the first insects to detect and interact with the carcasses, and six species of the Calliphoridae family were identified. Ants (Hymenoptera: Formicidae) were the second group, with six subfamilies identified. Myrmycinae represented 42% of the species, followed by Formicinae (28%), Ectatominae and Ponerinae (both 10%), and Ecitoninae and Dolichoderinae (both 5%). The ants acted on the carcasses as predators of visiting species, omnivores, and necrophagous, in all cases significantly affecting the decomposition time, slowing it down when the ants preyed on adult and immature insects consuming the carcass, or accelerating it by consuming the carcass and creating holes that could serve as gateways for the action of other organisms. The ants also generated artifacts that could lead to forensic misinterpretation.

  9. Response of Lucilia sericata (Diptera: Calliphoridae) to Screwworm Oviposition Attractant.

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    Chaudhury, M F; Zhu, J J; Skoda, S R

    2015-07-01

    The sheep blowfly, Lucilia sericata Meigen (Diptera: Calliphoridae), causes sheep myiasis in various parts of the world. Female flies are attracted to sheep following various olfactory cues emanating from the sheep's body, and oviposit on suitable substrates on sheep ultimately causing myiasis. Earlier workers attempted to reduce fly population in the field, with some success, using traps baited with various attractants. This research was conducted to determine if L. sericata would respond to a recently developed synthetic attractant that has attracted gravid screwworms, Cochliomyia hominivorax Coquerel, and stimulated them to oviposit. Results of the laboratory bioassays demonstrated that gravid females L. sericata were attracted to substrates treated with the synthetic screwworm attractant composed of five compounds--dimethyl disulfide, dimethyl trisulfide, phenol, p-cresol, and indole. Tests with various combinations of these compounds suggest that the sulfur compounds and indole are the most important compounds to elicit attraction and stimulate oviposition, while phenol and p-cresol may have minor roles. Semiochemical baits based on these compounds may be useful in the field to trap gravid L. sericata. Published by Oxford University Press on behalf of Entomological Society of America 2015. This work is written by US Government employees and is in the public domain in the US.

  10. Abundance and seasonality of Cochliomyia macellaria (Diptera: Calliphoridae) in Southern Pantanal, Brazil Sazonalidade de Cochliomyia macellaria (Diptera: Calliphoridae) no Pantanal Sul-mato-grossense, Brasil

    OpenAIRE

    2011-01-01

    Cochliomyia macellaria (Diptera: Calliphoridae), known as the secondary screwworm, occurs in the Americas and has medical-veterinary and forensic importance. This study aimed to describe the seasonal fluctuation of this species in the Pantanal region, Central-Western Brazil. From December 2004 to November 2007 fly catches using four windoriented traps baited with decaying bovine liver were carried out at the Nhumirim ranch, Nhecolândia subregion, Southern Pantanal. Traps remained active throu...

  11. What's in a frog stomach? Solving a 150 year old mystery (Diptera: Calliphoridae)

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    The taxon Acanthosoma chrysalis Mayer, 1844, described from Germany on a number of alleged parasites encysted in the peritoneal wall of the stomach of edible frogs, is revised and shown to be first instar larvae of blow flies (Calliphoridae). Based on the shape of mouthhooks and abdominal cuticular ...

  12. Morfologia e duração do desenvolvimento pós-embrionário de Chrysomya megacephala (Diptera: Calliphoridae em condições de laboratório

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    Karine Brenda Barros-Cordeiro

    2010-01-01

    Full Text Available A morfologia e o tempo de desenvolvimento pós-embrionário de Chrysomya megacephala (Fabricius criada em condições de laboratório e em dieta de carne bovina, foi descrita e comparada ao de outras espécies. Os parâmetros analisados foram à duração dos instares larvais e a morfologia. Para isso as larvas foram fixadas e preservadas em intervalos de tempo de 2 horas, desde a eclosão das larvas até a 50ª hora, a fim de se determinar o tempo de desenvolvimento de cada estágio. O tempo total de desenvolvimento larval para C. megacephala foi de 98 horas, com média de comprimento 15,51 mm. Essa espécie tem comportamento necrobiontófago e de importância sanitária e médico-legal, usada na estimativa do intervalo post-mortem em investigação criminal.Morphology and duration of the post-embryonary development of Chrysomya megacephala (Diptera: Calliphoridae in laboratory conditions. The morphology and developmental time of the post-embryonic stage of Chrysomya megacephala (Fabricius reared in laboratory on a beef diet, is described and compared to those of other blow-flies species. The parameters analyzed were the duration of the larval stages and the morphology. Larvae were fixed and preserved at 2 hours intervals, from larval eclosion until the 50th hour of life, in order to determine the duration of each stage. The total period of larval development in C. megacephala was 98 hours and their mean length was 15.51 mm. This species presents a necrobiontophagous behavior and has sanitary and medico-legal importance, being used for estimating postmortem interval in criminal investigations.

  13. Repellent Action of Carapa guianensis and Caesalpinia ferrea for flies species of Calliphoridae family

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    Ciciane Pereira Marten Fernandes

    2016-05-01

    Full Text Available ABSTRACT: Myiases occur by the infestation of fly larvae in tissues of live vertebrate animals, resulting in economic loss. Phytotherapy is considered an important alternative in the control of insects, which may reduce the economic impacts . Carapa guianensis is a plant that has been studied as a repellent against mosquitoes and Caesalpinia ferrea is reported in tropical climates, and there are few studies about its repellent action. The present study was designed to evaluate the repellent action of s C. guianensis and C. ferrea plants on flies species of the Calliphoridae family. W.O.T. traps containing deteriorated bovine liver and herbs cream of at concentrations of 20 and 50% were used to catch the flies. It was reported that the creams containing C. ferrea at concentrations of 20 and 50% and C. guianensis at the concentration of 50% have repellent effect against species of Calliphoridae family.

  14. Abundance and seasonality of Cochliomyia macellaria (Diptera: Calliphoridae in Southern Pantanal, Brazil Sazonalidade de Cochliomyia macellaria (Diptera: Calliphoridae no Pantanal Sul-mato-grossense, Brasil

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    Wilson Werner Koller

    2011-03-01

    Full Text Available Cochliomyia macellaria (Diptera: Calliphoridae, known as the secondary screwworm, occurs in the Americas and has medical-veterinary and forensic importance. This study aimed to describe the seasonal fluctuation of this species in the Pantanal region, Central-Western Brazil. From December 2004 to November 2007 fly catches using four windoriented traps baited with decaying bovine liver were carried out at the Nhumirim ranch, Nhecolândia subregion, Southern Pantanal. Traps remained active throughout the study period and collections were carried out on a weekly basis. A total of 159,397 Calliphoridae were caught and C. macellaria (57.33% was the most abundant species. C. macellaria occurred all over the year showing a bimodal behavior with peaks in May-July (late autumn/early winter and October-December (spring.Cochliomyia macellaria (Diptera: Calliphoridae, conhecida como mosca-varejeira, ocorre no continente americano e apresenta importância médico-veterinária e forense. O presente estudo teve como objetivo conhecer a flutuação sazonal dessa espécie na região do Pantanal. De dezembro/2004 a novembro/2007 foram realizadas coletas de dípteros na fazenda Nhumirim, sub-região da Nhecolândia, Pantanal sul-mato-grossense. Foram utilizadas quatro armadilhas orientadas pelo vento, iscadas com fígado bovino deteriorado. As armadilhas permaneceram ativas durante todo o período de estudo, e coletas foram realizadas semanalmente. Foram capturados 159.397 califorídeos, sendo C. macellaria (57,33% a espécie mais abundante. C. macellaria foi observada em todos os meses do ano, apresentando comportamento bimodal com picos populacionais em maio/julho (final de outono/início de inverno e outubro/dezembro (primavera.

  15. Checklist and distribution maps of the blow flies of Venezuela (Diptera, Calliphoridae, Mesembrinellidae).

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    Velásquez, Yelitza; Martínez-Sánchez, Ana Isabel; Thomas, Arianna; Rojo, Santos

    2017-01-01

    A checklist of the 39 species of blow flies (Calliphoridae and Mesembrinellidae) so far known to occur in Venezuela is provided, based on a thorough literature review and the examination of ca. 500 specimens deposited in the main entomological collections of the country. Data from the literature and museum collections were used to generate distribution maps for 37 species. Three species are recorded from Venezuela for the first time: Chrysomya putoria (Wiedemann, 1830), Mesembrinella spicata Aldrich, 1925 and Mesembrinella umbrosa Aldrich, 1922.

  16. Determination of Calliphoridae (Diptera) fauna and seasonal distribution on carrion in Ankara province.

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    Sabanoğlu, Burcu; Sert, Osman

    2010-07-01

    This study was conducted from March 2006 to 2007. The aim of the study was to determine the forensically significant Calliphoridae (Diptera) species and their seasonal distribution in Ankara province at Beytepe Campus. Pig carcasses were killed by a veterinary using pentobarbital sodium with intervals from 2 to 3 weeks to a month. Samples were collected from the carcass everyday in the morning, in the afternoon, and at sunset. Temperature and parameters of weather, such as cloudy, sunny, rainy, and snowy, were recorded at each visit. Phaenicia sericata (Meigen), Chrysomya albiceps (Wiedemann), Calliphora vomitoria (Linnaeus), and Calliphora vicina (Robineau-Desvoidy) species, which belong to Calliphoridae family, were collected. It was shown that the seasonal distribution of the collected species was different from each other. The species were determined on carcass between the following times; P. sericata, from April to November; C. albiceps, from May to November; Ca. vomitoria, from February to June and from September to December; and Ca. vicina, from February to May and in June and September. Calliphoridae fauna and its annual seasonal distribution in Turkey on 12 pig carcasses were reported for the first time in this study.

  17. Scanning Electron Microscopy Investigations of Third-Instar Larva of Cordylobia rodhaini (Diptera: Calliphoridae), an Agent of Furuncular Myiasis.

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    Pezzi, M; Cultrera, R; Chicca, M; Leis, M

    2015-05-01

    A scanning electron microscopy study of the third larval instar of Cordylobia rodhaini Gedoelst (Diptera: Calliphoridae), causing obligatory furuncular myiasis, is presented here for the first time. The larvae were collected from a patient exposed to them in the tropical rainforest of Kibale National Park (Uganda). Distinctive features are described in sequence from the anterior region to the posterior region, highlighting the morphological features of antennae, maxillary palps, structures related to mouth opening, sensory structures, thoracic and abdominal spines, and anterior and posterior spiracles. The results are compared with those of other Calyptrata flies, mainly from the family Calliphoridae and, when possible, with Cordylobia anthropophaga Blanchard (Diptera: Calliphoridae), the only other species of genus Cordylobia investigated by scanning electron microscopy.

  18. Checklist and distribution maps of the blow flies of Venezuela (Diptera, Calliphoridae, Mesembrinellidae)

    Science.gov (United States)

    Velásquez, Yelitza; Martínez-Sánchez, Ana Isabel; Thomas, Arianna; Rojo, Santos

    2017-01-01

    Abstract A checklist of the 39 species of blow flies (Calliphoridae and Mesembrinellidae) so far known to occur in Venezuela is provided, based on a thorough literature review and the examination of ca. 500 specimens deposited in the main entomological collections of the country. Data from the literature and museum collections were used to generate distribution maps for 37 species. Three species are recorded from Venezuela for the first time: Chrysomya putoria (Wiedemann, 1830), Mesembrinella spicata Aldrich, 1925 and Mesembrinella umbrosa Aldrich, 1922. PMID:28228670

  19. Prospect of a method of infected wound healing with a help of Calliphoridae blowfly screwworms

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    L. I. Faly

    2012-02-01

    Full Text Available Complex assessment of possibilities of infected wound healing with a help of screwworms of blowfly of the family Calliphoridae in the hospitals of Ukraineis offered. On the basis of available data the mechanism of therapeutic action is shown, and the medicinal properties of substances containing in the secretions and excretions of the blowfly screwworms are described. The gist of the treatment method and traits of the blowfly cultivation in a laboratory are highlighted. The quality standards for specialized biological laboratories are presented. The proposed wound healing method is proved as promising.

  20. New locality record of Isomyia paurogonita Fang & Fan, 1986 (Diptera: Calliphoridae) from Peninsular Malaysia and Borneo.

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    Heo, C C; Aisha, S; Kurahashi, H; Omar, B

    2013-03-01

    Isomyia paurogonita Fang & Fan, 1986 (Diptera: Calliphoridae), a rare species of the subfamily Rhiniinae (tribe Cosminini) was recorded for the first time in Malaysia. We collected one male and two females during a field trip conducted at Genting Highland, Pahang, peninsular Malaysia in May 2011. A 3-day old cow liver was offered as attractant and dipterans collected were transferred to the laboratory for specimens processing and identification. The adults of I. paurogonita were attracted to the odour and then captured by using a sweep net. Isomyia paurogonita was also recorded from two other localities in Peninsular and Malaysian Borneo, namely Gombak Utara, Selangor and Sibu, Sarawak.

  1. Parasitoid wasps (Hymenoptera from puparia of sarcosaprophagous flies (Diptera: Calliphoridae; Sarcophagidae in Buenos Aires, Argentina

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    Adriana OLIVA

    2008-01-01

    Full Text Available Se registró la emergencia de parasitoides (Hymenoptera de crías experimentales de Diptera sarcosaprófagas (Calliphoridae, Sarcophagidae, atraídas a cebos de carne bovina, en Buenos Aires (Argentina durante 1998-2003. Se determinaron cuatro taxones: Tachinaephagus zealandicus Ashmead (Encyrtidae, Brachymeria podagrica (Fabricius (Chalcididae, Nasonia vitripennis (Walker (Pteromaliidae y Alysia sp. (Braconidae: Alysiinae. Sólo las dos primeras especies resultaron abundantes en todos los años. Se ha graficado el número total de emergencias de cada especie para cada mes, junto con las temperaturas promedio máxima y mínima.

  2. Beyond barcoding: a mitochondrial genomics approach to molecular phylogenetics and diagnostics of blowflies (Diptera: Calliphoridae).

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    Nelson, Leigh A; Lambkin, Christine L; Batterham, Philip; Wallman, James F; Dowton, Mark; Whiting, Michael F; Yeates, David K; Cameron, Stephen L

    2012-12-15

    Members of the Calliphoridae (blowflies) are significant for medical and veterinary management, due to the ability of some species to consume living flesh as larvae, and for forensic investigations due to the ability of others to develop in corpses. Due to the difficulty of accurately identifying larval blowflies to species there is a need for DNA-based diagnostics for this family, however the widely used DNA-barcoding marker, cox1, has been shown to fail for several groups within this family. Additionally, many phylogenetic relationships within the Calliphoridae are still unresolved, particularly deeper level relationships. Sequencing whole mt genomes has been demonstrated both as an effective method for identifying the most informative diagnostic markers and for resolving phylogenetic relationships. Twenty-seven complete, or nearly so, mt genomes were sequenced representing 13 species, seven genera and four calliphorid subfamilies and a member of the related family Tachinidae. PCR and sequencing primers developed for sequencing one calliphorid species could be reused to sequence related species within the same superfamily with success rates ranging from 61% to 100%, demonstrating the speed and efficiency with which an mt genome dataset can be assembled. Comparison of molecular divergences for each of the 13 protein-coding genes and 2 ribosomal RNA genes, at a range of taxonomic scales identified novel targets for developing as diagnostic markers which were 117-200% more variable than the markers which have been used previously in calliphorids. Phylogenetic analysis of whole mt genome sequences resulted in much stronger support for family and subfamily-level relationships. The Calliphoridae are polyphyletic, with the Polleninae more closely related to the Tachinidae, and the Sarcophagidae are the sister group of the remaining calliphorids. Within the Calliphoridae, there was strong support for the monophyly of the Chrysomyinae and Luciliinae and for the sister

  3. Methods for external disinfection of blow fly (Diptera: Calliphoridae) eggs prior to use in wound debridement therapy

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    Maggot debridement therapy (MDT) is the use of the larval stage of flies (i.e., Calliphoridae) to remove necrotic tissue and disinfect wounds. Effective MDT requires an aseptic technique to prevent the unintentional introduction of pathogenic bacteria into a wound to be debrided, yet the external s...

  4. Comparative morphology of the spermathecae of some species of Chrysomya Robineau-Desvoidy and Cochliomyia Townsend (Diptera, Calliphoridae

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    Érica Sevilha Harterreiten-Souza

    2012-03-01

    Full Text Available Comparative morphology of the spermathecae of some species of Chrysomya Robineau-Desvoidy and Cochliomyia Townsend (Diptera, Calliphoridae. Little is known about the morphology of the chitinized structures of the spermathecae of the Calliphoridae. In this work, the spermathecae of Chrysomya albiceps Wiedemann, 1819, C. megacephala Fabricius, 1794, Cochliomyia macellaria Fabricius, 1775 and C. hominivorax Coquerel, 1858 are described and illustrated. The occurrence in one species of four spermathecae, an atypical form for blow flies, was recorded for the first time. The analysis of these structures will allow a better understanding of this group as well as provide taxonomic characters for future phylogenetic studies.Morfologia comparada das espermatecas de espécies de Chrysomya Robineau-Desvoidy e Cochliomyia Townsend (Diptera, Calliphoridae. Pouco se conhece sobre a morfologia das estruturas quitinizadas das espermatecas de Calliphoridae. Nesse trabalho as espermatecas de Chrysomya albiceps Wiedemann, 1819, C. megacephala Fabricius, 1794, Cochliomyia macellaria Fabricius, 1775 e C. hominivorax Coquerel, 1858 são descritas e ilustradas. Foi registrada pela primeira vez a ocorrência em uma espécie com quatro espermatecas, uma forma atípica em califorídeos. A análise dessas estruturas possibilitará uma melhor compreensão do grupo e fornecerá caracteres taxonômicos para futuros estudos filogenéticos.

  5. ANÁLISIS GENÉTICO DE CARACTERÍSTICAS REPRODUCTIVAS DE VACAS HOLSTEIN CRIADAS EN UN AMBIENTE SUBTROPICAL

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    \\u00C1ngel R\\u00EDos-Utrera

    2010-01-01

    Full Text Available El objetivo del presente trabajo fue estimar la heredabilidad y la repetibilidad de características reproductivas de vacas Holstein criadas en condiciones subtropicales. Se analizaron los registros de 171 hembras, las cuales fueron hijas de 67 sementales y 100 vacas. Las hembras nacieron de 1986 a 2006, en el sitio experimental Las Margaritas del Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP, en Hueytamalco, Puebla, México. Los parámetros genéticos fueron estimados para el intervalo parto-primer calor, intervalo parto-primer servicio, número de servicios por concepción, días abiertos e intervalo entre partos. Los componentes de varianza para el intervalo parto-primer calor, intervalo parto-primer servicio, intervalo entre partos y días abiertos fueron estimados con un modelo animal de repetibilidad para una sola característica, mientras que los componentes de varianza para número de servicios por concepción fueron estimados con un modelo animal simple, el que sólo incluyó el efecto genético aditivo individual como efecto aleatorio. Las estimaciones de los componentes de varianza se hicieron mediante máxima verosimilitud restringida libre de derivadas, utilizando el programa MTDFREML. Las estimaciones de heredabilidad fueron bajas, de 0,01 (días abiertos a 0,13 (intervalo entre partos, al igual que las de repetibilidad, que fueron de 0,11 (intervalo parto-primer servicio a 0,16 (días abiertos. En general, los estimados de heredabilidad y repetibilidad obtenidos están dentro de los intervalos reportados en la literatura científica. La magnitud de los estimados indica que la respuesta directa a la selección para una sola característica sería lenta.

  6. Using the Developmental Gene Bicoid to Identify Species of Forensically Important Blowflies (Diptera: Calliphoridae

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    Seong Hwan Park

    2013-01-01

    Full Text Available Identifying species of insects used to estimate postmortem interval (PMI is a major subject in forensic entomology. Because forensic insect specimens are morphologically uniform and are obtained at various developmental stages, DNA markers are greatly needed. To develop new autosomal DNA markers to identify species, partial genomic sequences of the bicoid (bcd genes, containing the homeobox and its flanking sequences, from 12 blowfly species (Aldrichina grahami, Calliphora vicina, Calliphora lata, Triceratopyga calliphoroides, Chrysomya megacephala, Chrysomya pinguis, Phormia regina, Lucilia ampullacea, Lucilia caesar, Lucilia illustris, Hemipyrellia ligurriens and Lucilia sericata; Calliphoridae: Diptera were determined and analyzed. This study first sequenced the ten blowfly species other than C. vicina and L. sericata. Based on the bcd sequences of these 12 blowfly species, a phylogenetic tree was constructed that discriminates the subfamilies of Calliphoridae (Luciliinae, Chrysomyinae, and Calliphorinae and most blowfly species. Even partial genomic sequences of about 500 bp can distinguish most blowfly species. The short intron 2 and coding sequences downstream of the bcd homeobox in exon 3 could be utilized to develop DNA markers for forensic applications. These gene sequences are important in the evolution of insect developmental biology and are potentially useful for identifying insect species in forensic science.

  7. Diversity and synanthropy of Calliphoridae (Diptera) in the region of Rio Claro, SP, Brazil.

    Science.gov (United States)

    De Souza, C R; Zuben, C J V

    2012-06-01

    Dipteran blowflies (Calliphoridae) are of great medical and hygienic importance as vectors of pathogens and as parasites of living and dead tissue, and their association with carrion allows their use in forensic entomology. The objective of this study was to determine the synanthropic index of adult Calliphoridae (Diptera) collected in Rio Claro, São Paulo. Sampling occurred between September 2009 and August 2010. Traps baited with sardines, beef liver, and minced meat were assessed for five consecutive days per month in three distinct ecological areas representing urban, rural, and forest environments. The most abundant species was Lucilia eximia (Wiedemann), followed by Chrysomya albiceps (Wiedemann) and Chrysomya megacephala (Fabricius). Lucilia eximia was the only species present in all seasons and the only species collected during the winter. The season with the lowest abundance was winter, with 69 (5.5%) specimens, and spring was the season with the greatest number of specimens collected (774-61.8%). The only species found outside inhabited areas (synanthropic) was Lucilia cuprina (Wiedemann), with a synanthropy index (SI) value of +5.7. The SI values for the other species were negative, showing a preference for uninhabited areas. The rural and urban areas were most similar in terms of species composition as were the beef and sardine baits. Among the baits used, liver attracted the greatest abundance of calliphorids, whereas minced meat attracted the greatest diversity.

  8. Forensically important calliphoridae (diptera) associated with pig carrion in rural north-central Florida

    Science.gov (United States)

    Gruner, Susan V.; Slone, D.H.; Capinera, J.L.

    2007-01-01

    A study to determine the relative abundance and seasonality of forensically important blow flies (Diptera: Calliphoridae) in rural north-central Florida was conducted using pig carcasses (Sus scrofa L.) as models for human bodies. Seven species of Calliphoridae were collected: Lucilia coeruleiviridis (=Phoenicia) (Macquart), Cochliomyia macellaria (F.), Chrysomya rufifaces (Macquart), Phormia regina (Meigen), Chrysomya megacephala (F.), and a few specimens of Calliphora livida Hall, and Calliphora vicina Robineau-Desvoidy. Species composition in aerial collections of adult flies, preserved larval collections, and samples of larvae reared to the adult stage were all highly correlated. Relative abundance of the species found was significantly different, with L. coeruleiviridis the most abundant species year-round. The relative abundance of the collected species varied significantly by day of decomposition and by season, with significant interactions between season and day, season and species, and day and species. L. coeruleiviridis, C. macellaria, C. rufifaces, and P. regina were found during the entire year, two C. vicina specimens and 11 C. livida specimens were collected from December to March, whereas C. megacephala was collected only from June through September. ?? 2007 Entomological Society of America.

  9. Genome sequence of Phormia regina Meigen (Diptera: Calliphoridae): implications for medical, veterinary and forensic research.

    Science.gov (United States)

    Andere, Anne A; Platt, Roy N; Ray, David A; Picard, Christine J

    2016-10-28

    Blow flies (Diptera: Calliphoridae) are important medical, veterinary and forensic insects encompassing 8 % of the species diversity observed in the calyptrate insects. Few genomic resources exist to understand the diversity and evolution of this group. We present the hybrid (short and long reads) draft assemblies of the male and female genomes of the common North American blow fly, Phormia regina (Diptera: Calliphoridae). The 550 and 534 Mb draft assemblies contained 8312 and 9490 predicted genes in the female and male genomes, respectively; including > 93 % conserved eukaryotic genes. Putative X and Y chromosomes (21 and 14 Mb, respectively) were assembled and annotated. The P. regina genomes appear to contain few mobile genetic elements, an almost complete absence of SINEs, and most of the repetitive landscape consists of simple repetitive sequences. Candidate gene approaches were undertaken to annotate insecticide resistance, sex-determining, chemoreceptors, and antimicrobial peptides. This work yielded a robust, reliable reference calliphorid genome from a species located in the middle of a calliphorid phylogeny. By adding an additional blow fly genome, the ability to tease apart what might be true of general calliphorids vs. what is specific of two distinct lineages now exists. This resource will provide a strong foundation for future studies into the evolution, population structure, behavior, and physiology of all blow flies.

  10. Review of Thompsoniella Guimarães with description of a new species from Colombia (Diptera, Calliphoridae, Mesembrinellinae

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    Marta Wolff

    2014-12-01

    Full Text Available Review of Thompsoniella Guimarães with description of a new species from Colombia (Diptera, Calliphoridae, Mesembrinellinae. The Mesembrinellinae (Diptera, Calliphoridae are exclusively Neotropical with nine genera comprising 36 recognized species, including the genus Thompsoniella Guimarães with a single species, T. anomala Guimarães. We describe a new species, Thompsoniella andina sp. nov., from the Departments of Antioquia and Caldas, Colombia (Cordillera Central of the Andes, between 2600 - 2700 m and redescribe T. anomala. A key to the nine genera of Mesembrinellinae and a key to the males of the two species of Thompsoniella are provided. Color photographs to illustrate the two species of Thompsoniella and drawings of the male genitalia of both species are also provided. Here we record Thompsoniella for the first time in Colombia.

  11. The blowflies of the Madeira Archipelago: species diversity, distribution and identification (Diptera, Calliphoridae s. l.)

    Science.gov (United States)

    Prado e Castro, Catarina; Szpila, Krzysztof; Martínez-Sánchez, Anabel; Rego; Silva, Isamberto; Serrano, Artur R.M.; Boieiro, Mário

    2016-01-01

    Abstract Knowledge on the taxonomic diversity and distribution of blowflies from the Madeira Archipelago is updated. New and interesting findings are reported for poorly studied islands and islets of this archipelago, together with a brief analysis of the diversity of Macaronesian Calliphoridae s. l. Seven blowfly species were collected during this study, including the first records of Calliphora vicina Robineau-Desvoidy, 1830, Chrysomya albiceps (Wiedemann, 1819), Lucilia sericata (Meigen, 1826), Pollenia rudis (Fabricius, 1794) and Stomorhina lunata (Fabricius, 1805) from Porto Santo, and of Calliphora vicina, Lucilia sericata and Stomorhina lunata from Desertas Islands. The presence of Calliphora loewi Enderlein, 1903 in Madeira Laurisilva forest is discussed and its first instar larva is redescribed, revealing important differences in relation to its original description. An identification key to the adult Madeiran blowflies is provided for the first time. PMID:27917052

  12. Morphology and Developmental Rate of the Blow Fly, Hemipyrellia ligurriens (Diptera: Calliphoridae: Forensic Entomology Applications

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    Nophawan Bunchu

    2012-01-01

    Full Text Available Hemipyrellia ligurriens (Diptera: Calliphoridae is a forensically important blow fly species presented in many countries. In this study, we determined the morphology of all stages and the developmental rate of H. ligurriens reared under natural ambient conditions in Phitsanulok province, northern Thailand. Morphological features of all stages based on observing under a light microscope were described and demonstrated in order to use for identification purpose. Moreover, development time in each stage was given. The developmental time of H. ligurriens to complete metamorphosis; from egg, larva, pupa to adult, took 270.71 h for 1 cycle of development. The results from this study may be useful not only for application in forensic investigation, but also for study in its biology in the future.

  13. Primer reporte de miasis hospitalaria por Lucilia cuprina (Diptera: Calliphoridae en Costa Rica

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    Lucía Quesada-Lobo

    2012-12-01

    Full Text Available Introducción. Las miasis hospitalarias son entidades con una importancia manifiesta en salud pública. La documentación de este tipo de casos es escasa en la literatura biomédica regional y mundial. Objetivo. Informar un caso de miasis hospitalaria en Costa Rica, donde el agente etiológico implicado fue Lucilia cuprina (Diptera: Calliphoridae. Este caso de miasis hospitalaria figura como el primer informe para Latinoamérica asociado con este agente etiológico. Presentación del caso. Una paciente de 91 años de edad, con signos de inmunosupresión, afectación grave de la función pulmonar y asistencia respiratoria mecánica, presentó larvas en ambas fosas nasales al séptimo día después del ingreso hospitalario. Varios ejemplares fueron recolectados y procesados para su identificación. La identificación taxonómica de los ejemplares recolectados estableció que la especie de los muscomorfos correspondía a L. cuprina. Conclusión. El presente constituye el primer caso de miasis hospitalaria por L. cuprina en la literatura biomédica de Costa Rica y el primero registrado en Latinoamérica.   doi: http://dx.doi.org/10.7705/biomedica.v32i4.690

  14. BACTERIA CARRIED BY CHRYSOMYA MEGACEPHALA (FABRICIUS, 1794 (DIPTERA: CALLIPHORIDAE IN SINOP, MATO GROSSO, BRAZIL

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    J. S. Carneiro

    2014-07-01

    Full Text Available Chrysomya megacephala (Diptera: Calliphoridae, popularly known as blowfly, has a great capacity for dispersion and, due to factors such as food abundance and favorable climate, it colonizes Brazil completely in a short time. These insects are important to the sectors of epidemiology, public health and forensics, especially due to carrying microorganisms such as bacteria, viruses, protozoa and helminthes, which are responsible for the spread of diseases such as dysentery, cholera, botulism, typhoid fever, brucellosis, polio, smallpox and tuberculosis. The objective of this study was to verify the diversity of bacteria carried by this species in the Federal University of Mato Grosso – Campus of Sinop during the month of January of 2012. The flies were collected using two traps baited with 100 g of fresh sardines on each and maintained in the field for 24 hours. Twenty specimens of C. megacephala were placed in Petri dishes, to walk for two minutes upon Nutrient Agar (NA. After establishment of the colonies, isolation of the bacteria on the NA medium and their multiplication in test tubes containing the same culture medium was performed, and later sent to identification by gas chromatography. The bacteria encountered were Aquaspirillum polymorphum; Burkholderia ambifaria; Burkholderia anthina; Burkholderia cepacia; Burkholderia cenocepacia; Burkholderia pyrrocinia; Burkholderia stabilis; Paenibacillus macerans; Virgibacillus pantothenticus, Bacillus subtilis e Photorhabdus luminescens luminescens, with the last two species considered of importance in the plant protection sector.

  15. Chemotaxonomic Profile and Intraspecific Variation in the Blow Fly of Forensic Interest Chrysomya megacephala (Diptera: Calliphoridae).

    Science.gov (United States)

    Paula, Michele C; Antonialli-Junior, William F; Mendonça, Angélica; Michelutti, Kamylla B; Eulalio, Aylson D M M; Cardoso, Claudia A L; de Lima, Thiago; Von Zuben, Cláudio J

    2017-01-01

    Necrophagous insects such as blow flies (Diptera: Calliphoridae) are considered crucial in forensic entomology. Identification at species level and determination of larval stage are the basis for estimation of postmortem interval (PMI). Insect evidence can also be used in the determination of crime scenes, since body displacement is common. The aim of this study was to determine the chemotaxonomic profile and intraspecific variability of the forensically important blow fly Chrysomya megacephala (F. 1794). Adults were collected in the municipalities of Dourados-MS (Brazil) and Rio Claro-SP (Brazil), and then transferred to the laboratory for oviposition and development of the immature stages. Chemical analysis of cuticular compounds was performed by gas chromatography. Cuticular chemical profiles varied significantly between the two populations, as well as between developmental stages, supporting the use of these compounds as a complementary tool to help identify the species and its stages, along with geographical variability. This could greatly accelerate forensic investigations, eliminating the need to allow the fly larvae to develop until adult stage in order to confirm the species identity and sample origin.

  16. Occurrence of blow fly species (Diptera: calliphoridae) in Phitsanulok Province, Northern Thailand.

    Science.gov (United States)

    Bunchu, Nophawan; Sukontason, Kom; Sanit, Sangob; Chidburee, Polprecha; Kurahashi, Hiromu; Sukontason, Kabkaew L

    2012-12-01

    Based on the current forensic importance of blow flies (Diptera: Calliphoridae), their biological aspects have been studied increasingly worldwide. The blow fly fauna in Phitsanulok Province, Northern Thailand was studied from May 2009 to April 2010 in the residential, agricultural, mountainous and forested areas of Muang, Wat Bot, Nakhon Thai and Wang Thong districts, respectively, in order to know the occurrence of blow flies in this province. Collections were carried out monthly using commercial funnel fly traps and sweeping methods, with 1-day tainted pork viscera as bait. Identification of adult blow flies exhibited 14 634 specimens, comprising of 5 subfamilies, 14 genera and 36 species. Chrysomya megacephala (Fabricius, 1794) and Achoetandrus rufifacies (Macquart, 1843) were the most and second most abundant species trapped, respectively. These two species of carrion flies prevailed in all the types of land investigated. We calculated and compared the diversity indices, species evenness and richness, and similarity coefficients of the blow fly species in various areas. The data from this study may be used to identify the potential of forensicallyimportant fly species within Phitsanulok Province and fulfill the information on blow fly fauna in Thailand.

  17. Effect of age on cuticular hydrocarbon profiles in adult Chrysomya putoria (Diptera: Calliphoridae).

    Science.gov (United States)

    Braga, Marina Vianna; Pinto, Zeneida Teixeira; Queiroz, Margareth Maria de Carvalho; Blomquist, Gary James

    2016-02-01

    A species-specific complex mixture of highly stable cuticular hydrocarbons (CHCs) covers the external surface of all insects. Components can be readily analyzed by gas chromatography coupled to mass spectrometry (GC-MS) to obtain a cuticular hydrocarbon profile, which may be used as an additional tool for the taxonomic differentiation of insect species and also for the determination of the age and sex of adult and immature forms. We used GC-MS to identify and quantify the CHCs of female and male Chrysomya putoria (Wiedemann, 1818) (Diptera: Calliphoridae) from one to five days old. CHCs ranged from C21 to C35 for females and from C21 to C37 in males. Major compounds were the same for both sexes and were 2-MeC28, C29:1, n-C29, 15-,13-MeC29, 2-MeC30, C31:1, n-C31 and 15-,13-MeC31. The relative abundance of each component, however, varied with age. Cluster Analysis using Bray-Curtis measure for abundance showed that cuticular hydrocarbon profiles are a strong and useful tool for the determination of age in adult C. putoria.

  18. Blowflies (Diptera, Calliphoridae) Associated with Pig Carcasses in a Caatinga Area, Northeastern Brazil.

    Science.gov (United States)

    Alves, A C F; Santos, W E; Farias, R C A P; Creão-Duarte, A J

    2014-04-01

    Studies that focused on Calliphoridae associated with pig carcasses are abundant in southern and southeastern Brazil; however, there are few in northeast. Here, we present an inventory of the blowfly species associated with the stages of decomposition of pig carcasses in a caatinga area during dry and rainy seasons. The study took place at the Private Reserve for the Environmental Inheritance "Fazenda Almas," state of Paraíba, Brazil. Using a modified version of the Shannon trap, 32,909 adult specimens belonging to eight species were captured. During the dry season, Cochliomyia macellaria (Fabricius) (52.2%) and Chrysomya albiceps (Wiedemann) (39.9%) were the most abundant species. In the rainy season, when the majority of individuals were captured (93.7%), Chloroprocta idioidea (Robineau-Desvoidy) (71.1%) was the most abundant. Five decomposition stages were recognized, being the active decay the most attractive to colonization by blowflies, except for Lucilia eximia (Wiedemann), which was more abundant in the bloated stage.

  19. A new species of Giovanella Bonatto (Diptera, Calliphoridae, Mesembrinellinae from Colombia

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    Marta Wolff

    2013-06-01

    Full Text Available A new species of Giovanella Bonatto (Diptera, Calliphoridae, Mesembrinellinae from Colombia. The Mesembrinellinae are exclusively Neotropical, with 33 nominal species distributed in nine genera. Only the genera Mesembrinella, Eumesembrinella, Huascaromusca and Laneella had until now been recorded in Colombia. In this work we present a new species of Giovanella Bonatto, 2005, genus with only one species, G. bolivar Bonatto, differing from the new species G. carvalhoi sp. nov., in the characters: legs entirely dark chestnut; thorax with dorsocentrals setae 1:2; abdomen with T5 with discal bristles poorly differentiated; T6 symmetric and paraphallus not extended and with denticules. Giovanella carvalhoi sp. nov. is from Cordillera Oriental, from the Departments of Caquetá (Amazonian foothills and Santander, Colombia, collected between 22002400 m., and associated with decomposing organic matter. A key for the identification of males and females of the two species of Giovanella, illustrations of the genitalia and photographs of male and female of new species are also presented.

  20. Ultrastructure of immature stages of Cochliomyia macellaria (Diptera: Calliphoridae), a fly of medical and veterinary importance.

    Science.gov (United States)

    Mendonça, Paloma Martins; Barbosa, Rodrigo Rocha; Cortinhas, Lucas Barbosa; dos Santos-Mallet, Jacenir Reis; de Carvalho Queiroz, Margareth Maria

    2014-10-01

    Cochliomyia macellaria (Diptera: Calliphoridae) is known as the secondary screwworm because it causes secondary or facultative myiasis when the larvae feed on necrotic tissues. This fly has a significant medical and veterinary importance since it has been reported to transport eggs of Dermatobia hominis (human botfly), which can cause significant economic losses to livestock. Since this screwworm has been collected colonizing both pig carcasses and human cadavers, it is considered one of the most important species for forensic entomology studies. Scanning electron microscopy (SEM) gives detailed information on the morphological characteristics which can help identify the immature forms of the flies. The aim of this study was to describe and analyze the morphological characteristics of the eggs, all the larval instars, and the puparia of Cochliomyia macellaria using SEM. The egg is ellipsoid and the dorsal surface is concave. The islands inside the median area had no anastomosis, but some perforations could be observed. From the second larval instar onwards, besides the intersegmental spines, other bands of spines were observed at the abdominal segments. Two spiracular openings were visible on the first and second larval instars, which were not expected. These characteristics are specific to Cochliomyia genus. The number and the general aspect of the spine tips in the cephalic region, the intersegmental bands on the abdomen, and the number of the spiracular openings could together help identify C. macellaria.

  1. Methods for external disinfection of blow fly (Diptera: Calliphoridae) eggs prior to use in wound debridement therapy.

    Science.gov (United States)

    Brundage, Adrienne L; Crippen, Tawni L; Tomberlin, Jeffery K

    2016-03-01

    Maggot debridement therapy (MDT) is the use of the larval stage of flies (i.e., Calliphoridae) to remove necrotic tissue and disinfect wounds. Effective MDT requires aseptic technique to prevent the unintentional introduction of pathogenic bacteria into a wound to be debrided; yet the external surface of Calliphoridae eggs is often heavily contaminated with bacteria. Studies of external disinfection of dipteran eggs have been reported, but neither their efficacy nor effect on egg viability has been adequately assessed. The present study evaluated the efficacy of ten disinfection techniques involving immersion, rinse, or a combination of both in formalin, Lysol, formaldehyde, bleach, ethanol, Sporgon, or benzalkonium chloride. All techniques resulted in significant decreases in culturable, aerobic bacterial load on Lucilia cuprina eggs. For L. cuprina, a 10 minute 3% Lysol immersion was the most efficacious, disinfecting 96.67% of egg samples, while resulting in 31.84% egg mortality. The 5% formalin immersion was least efficacious, disinfecting only 3.33% of L. cuprina egg samples, while resulting in 33.51% egg mortality. A formaldehyde immersion, one of the most commonly used disinfection techniques, was moderately effective, disinfecting 66.7% of egg samples, while resulting in 40.16% egg mortality. For Chrysomya rufifacies and Cochliomyia macellaria egg samples, the 10 minute 3% Lysol immersion disinfected 100% of the samples, and for Lucilia sericata, 80% of egg samples, while resulting in 33.97%, 7.34%, and 36.96% egg mortality, respectively. H2 CO disinfected 16.67% of Ch. rufifacies, 26.67% of C. macellaria, and 56.67% of L. sericata egg samples, while resulting in 21.98%, 10.18%, and 32.19% egg mortality, respectively. Due to its high disinfection efficacy and relatively low egg mortality, a 10 minutes 3% Lysol immersion is recommended for sterilizing Calliphoridae eggs prior to rearing of larvae for use in MDT.

  2. ITS2 PARA LA IDENTIFICACIÓN DE CALIFÓRIDOS (DIPTERA: CALLIPHORIDAE DE IMPORTANCIA FORENSE EN COLOMBIA

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    Edison R. LEA-CHARRIS

    2016-01-01

    Full Text Available La entomología forense es una disciplina que utiliza insectos para obtener información útil en la determinación del intervalo postmortem (IPM. Las moscas de la familia Calliphoridae son muy utilizadas en entomología forense, sin embargo, su identificación a nivel de especie puede dificultarse cuando el individuo se encuentra incompleto o en estadio inmaduro. En el presente trabajo, se evaluó el potencial de la región ITS2 del genoma nuclear para la identificación de especies de Calliphoridae en Colombia utilizando tres aproximaciones: comparando distancias genéticas utilizando la metodología de códigos de barra, haciendo una reconstrucción filogenética, y con enzimas de restricción (PCR-RFLPs. Se secuenciaron un total de 520 pb en 44 individuos pertenecientes a 16 especies. Se calcularon los valores de distancia intraespecífica e interespecíficas utilizando el modelo K2P. Los valores de distancia intraespecífica oscilaron entre 0 y 0,252 %, mientras que las distancias interespecíficas fluctuaron entre 3,6 y 18,9 %, evidenciándose que esta técnica puede ser utilizada como código de barras genético en la identificación de especies de la familia Calliphoridae. Tanto en los análisis de Neighbour-Joining como en los análisis bayesianos el 90 % de los géneros presentan una monofilia sustentada en probabilidad posterior de 0,89 a 1. En todos los casos la especie Blepharicnema splendens agrupa con el género Lucilia. Con base en las secuencias obtenidas se utilizó la aplicación NEBCutter para identificar cuatro enzimas de restricción las cuales se probaron en el laboratorio y se comprobó su utilidad para la identificación rápida de especies de Calliphoridae en Colombia.

  3. ITS2 para la identificación de Califóridos (Diptera: Calliphoridae de importancia forense en Colombia

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    Edison R. Lea-Charris

    2016-09-01

    Full Text Available La entomología forense es una disciplina que utiliza insectos para obtener información útil en la determinación del intervalo postmortem (IPM. Las moscas de la familia Calliphoridae son muy utilizadas en entomología forense, sin embargo, su identificación a nivel de especie puede dificultarse cuando el individuo se encuentra incompleto o en estadio inmaduro. En el presente trabajo, se evaluó el potencial de la región ITS2 del genoma nuclear para la identificación de especies de Calliphoridae en Colombia utilizando tres aproximaciones: comparando distancias genéticas utilizando la metodología de códigos de barra, haciendo una reconstrucción filogenética, y con enzimas de restricción (PCR-RFLPs. Se secuenciaron un total de 520 pb en 44 individuos pertenecientes a 16 especies. Se calcularon los valores de distancia intraespecífica e interespecíficas utilizando el modelo K2P. Los valores de distancia intraespecífica oscilaron entre 0 y 0,252 %, mientras que las distancias interespecíficas fluctuaron entre 3,6 y 18,9 %, evidenciándose que esta técnica puede ser utilizada como código de barras genético en la identificación de especies de la familia Calliphoridae. Tanto en los análisis de Neighbour-Joining como en los análisis bayesianos el 90 % de los géneros presentan una monofilia sustentada en probabilidad posterior de 0,89 a 1. En todos los casos la especie Blepharicnema splendens agrupa con el género Lucilia. Con base en las secuencias obtenidas se utilizó la aplicación NEBCutter para identificar cuatro enzimas de restricción las cuales se probaron en el laboratorio y se comprobó su utilidad para la identificación rápida de especies de Calliphoridae en Colombia.

  4. First Report of Myiasis Caused by Cochliomyia hominivorax (Diptera: Calliphoridae) in a Diabetic Foot Ulcer Patient in Argentina

    OpenAIRE

    Olea, María Sofía; Néstor CENTENO; Aybar, Cecilia Adriana Veggiani; Ortega, Eugenia Silvana; Galante, Guillermina Begoña; Olea, Luis; Juri, María Julia Dantur

    2014-01-01

    Myiasis is usually caused by flies of the Calliphoridae family, and Cochliomyia hominivorax is the etiological agent most frequently found in myiasis. The first case of myiasis in a diabetic foot of a 54-year-old male patient in Argentina is reported. The patient attended the hospital of the capital city of Tucumán Province for a consultation concerning an ulcer in his right foot, where the larval specimens were found. The identification of the immature larvae was based on their morphological...

  5. Parasitoid wasps (Hymenoptera from puparia of sarcosaprophagous flies (Diptera: Calliphoridae; Sarcophagidae in Buenos Aires, Argentina Avispas parasitoides (Hymenoptera a partir de puparios de moscas sarcosaprófagas (Diptera: Calliphoridae; Sarcophagidae en Buenos Aires, Argentina

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    Adriana Oliva

    2008-12-01

    Full Text Available Emergence of parasitoid Hymenoptera from experimental rearings of sarcosaprophagous Diptera (Calliphoridae, Sarcophagidae attracted to beef baits were recorded in Buenos Aires (Argentina from 1998 to 2003. Four taxa were identified: Tachinaephagus zealandicus Ashmead (Encyrtidae, Brachymeria podagrica (Fabricius (Chalcididae, Nasonia vitripennis (Walker (Pteromaliidae and Alysia sp. (Braconidae: Alysiinae. Only the first two species were abundant in all years. The number of added monthly emergences of each species is presented and correlated with monthly mean maximum-minimum temperatures.Se registró la emergencia de parasitoides (Hymenoptera de crías experimentales de Diptera sarcosaprófagas (Calliphoridae, Sarcophagidae, atraídas a cebos de carne bovina, en Buenos Aires (Argentina durante 1998-2003. Se determinaron cuatro taxones: Tachinaephagus zealandicus Ashmead (Encyrtidae, Brachymeria podagrica (Fabricius (Chalcididae, Nasonia vitripennis (Walker (Pteromaliidae y Alysia sp. (Braconidae: Alysiinae. Sólo las dos primeras especies resultaron abundantes en todos los años. Se ha graficado el número total de emergencias de cada especie para cada mes, junto con las temperaturas promedio máxima y mínima.

  6. Thermoregulation in larval aggregations of carrion-feeding blow flies (Diptera: Calliphoridae)

    Science.gov (United States)

    Slone, D.H.; Gruner, Susan V.

    2007-01-01

    The growth and development of carrion-feeding calliphorid (Diptera Calliphoridae) larvae, or maggots, is of great interest to forensic sciences, especially for estimation of a postmortem interval (PMI). The development rate of calliphorid larvae is influenced by the temperature of their immediate environment. Heat generation in larval feeding aggregations (=maggot masses) is a well-known phenomenon, but it has not been quantitatively described. Calculated development rates that do not include internally generated temperatures will result in overestimation of PMI. Over a period of 2.5 yr, 80 pig, Sus scrofa L., carcasses were placed out at study sites in north central Florida and northwestern Indiana. Once larval aggregations started to form, multiple internal and external temperatures, and weather observations were taken daily or every few days between 1400 and 1800 hours until pupation of the larvae. Volume of each aggregation was determined by measuring surface area and average depth. Live and preserved samples of larvae were taken for species identification. The four most common species collected were Lucilia coeruleiviridis (=Phaenicia) (Macquart) (77%), Cochliomyia macellaria (F.) (8.3%), Chrysomya rufifaces (Macquart) (7.7%), and Phormia regina (Meigen) (5.5%). Statistical analyses showed that 1) volume of a larval mass had a strong influence on its temperature, 2) internal temperatures of masses on the ground were influenced by soil temperature and mass volume, 3) internal temperatures of masses smaller than 20 cm3 were influenced by ambient air temperature and mass volume, and 4) masses larger than 20 cm3 on the carcass had strongly regulated internal temperatures determined only by the volume of the mass, with larger volumes associated with higher temperatures. Nonsignificant factors included presence of rain or clouds, shape of the aggregation, weight of the carcass, species composition of the aggregation, time since death, or season.

  7. Survey of the Genetic Diversity of Forensically Important Chrysomya (Diptera: Calliphoridae) from Egypt.

    Science.gov (United States)

    Salem, Abeer M; Adham, Fatma K; Picard, Christine J

    2015-05-01

    Minimum postmortem interval estimations of a corpse using blow fly larvae in medicolegal investigations require correct identification and the application of appropriate developmental data of the identified fly species. Species identification of forensically relevant blow flies could be very difficult and time consuming when specimens are damaged or in the event of morphologically indistinguishable immature stages, which are most common at crime scenes. In response to this, an alternative, accurate determination of species may depend on sequencing and molecular techniques for identification. Chrysomyinae specimens (n = 158) belonging to three forensically important species [Chrysomya albiceps (Wiedemann), Chrysomya megacephala (F.), and Chrysomya marginalis (Wiedemann)] (Diptera: Calliphoridae) were collected from four locations in Egypt (Giza, Dayrout, Minya, and North Sinai) and sequenced across the mitochondrial cytochrome oxidase subunit I (COI) gene. Phylogenetic analyses using neighbor-joining, maximum likelihood and maximum parsimony methods resulted in the same topological structure and confirmed DNA based identification of all specimens. Interspecific divergence between pairs of species was 5.3% (C. marginalis-C. megacephala), 7% (C. albiceps-C. megacephala), and 8% (C. albiceps-C. marginalis). These divergences are sufficient to confirm the utility of cytochrome oxidase subunit I gene in the molecular identification of these flies in Egypt. Importantly, the maximum intraspecific divergence among individuals within a species was <1% and the least nucleotide divergence between species used for phylogenetic analysis was 3.6%. This study highlights the need for thorough and diverse sampling to capture all of the possible genetic diversity if DNA barcoding is to be used for molecular identification.

  8. A metagenomic assessment of the bacteria associated with Lucilia sericata and Lucilia cuprina (Diptera: Calliphoridae).

    Science.gov (United States)

    Singh, Baneshwar; Crippen, Tawni L; Zheng, Longyu; Fields, Andrew T; Yu, Ziniu; Ma, Qun; Wood, Thomas K; Dowd, Scot E; Flores, Micah; Tomberlin, Jeffery K; Tarone, Aaron M

    2015-01-01

    Lucilia Robineau-Desvoidy (Diptera: Calliphoridae) is a blow fly genus of forensic, medical, veterinary, and agricultural importance. This genus is also famous because of its beneficial uses in maggot debridement therapy (MDT). Although the genus is of considerable economic importance, our knowledge about microbes associated with these flies and how these bacteria are horizontally and trans-generationally transmitted is limited. In this study, we characterized bacteria associated with different life stages of Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) and in the salivary gland of L. sericata by using 16S rDNA 454 pyrosequencing. Bacteria associated with the salivary gland of L. sericata were also characterized using light and transmission electron microscopy (TEM). Results from this study suggest that the majority of bacteria associated with these flies belong to phyla Proteobacteria, Firmicutes, and Bacteroidetes, and most bacteria are maintained intragenerationally, with a considerable degree of turnover from generation to generation. In both species, second-generation eggs exhibited the highest bacterial phylum diversity (20 % genetic distance) than other life stages. The Lucilia sister species shared the majority of their classified genera. Of the shared bacterial genera, Providencia, Ignatzschineria, Lactobacillus, Lactococcus, Vagococcus, Morganella, and Myroides were present at relatively high abundances. Lactobacillus, Proteus, Diaphorobacter, and Morganella were the dominant bacterial genera associated with a survey of the salivary gland of L. sericata. TEM analysis showed a sparse distribution of both Gram-positive and Gram-negative bacteria in the salivary gland of L. sericata. There was more evidence for horizontal transmission of bacteria than there was for trans-generational inheritance. Several pathogenic genera were either amplified or reduced by the larval feeding on decomposing liver as a resource. Overall, this study provides

  9. Fly artifact documentation of Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) - a forensically important blowfly species in Malaysia.

    Science.gov (United States)

    Zuha, R M; Supriyani, M; Omar, B

    2008-04-01

    Analysis on fly artifacts produced by forensically important blowfly, Chrysomya megacephala (Fabricius) (Diptera:Calliphoridae), revealed several unique patterns. They can be divided into fecal spots, regurgitation spots and swiping stains. The characteristics of fecal spots are round with three distinct levels of pigmentation; creamy, brownish and darkly pigmented. Matrix of the spots appears cloudy. The round spots are symmetrical and non-symmetrical, delineated by irregular and darker perimeter which only visible in fairly colored fecal spots. Diameter of these artifacts ranged from 0.5 mm to 4 mm. Vomit or regurgitation spots are determined by the presence of craters due to sucking activity of blowflies and surrounded by thickly raised and darker colored perimeter. The size of these specks ranged from 1 mm to 2 mm. Matrix of the spots displays irregular surface and reflective under auxiliary microscope light. Swiping stains due to defecation by flies consists of two distinguishable segments, the body and tail. It can be seen as a tear drop-like, sperm-like, snake-like and irregular tadpole-like stain. The direction of body and tail is inconsistent and length ranged between 4.8 mm to 9.2 mm. A finding that should be highlighted in this observation is the presence of crater on tadpole-like swiping stain which is apparent by its raised border characteristic and reflective under auxiliary microscope light. The directionality of this darkly brown stain is random. This unique mix of regurgitation and swiping stain has never been reported before. Highlighting the features of artifacts produced by flies would hopefully add our understanding in differentiating them from blood spatters produced from victims at crime scenes.

  10. Impact of abiotic factor changes in blowfly, Achoetandrus rufifacies (Diptera: Calliphoridae), in northern Thailand.

    Science.gov (United States)

    Klong-Klaew, Tunwadee; Sukontason, Kom; Ngoen-klan, Ratchadawan; Moophayak, Kittikhun; Irvine, Kim N; Kurahashi, Hiromu; Prangkio, Chira; Sanit, Sangob; Sukontason, Kabkaew L

    2014-04-01

    Understanding how medically important flies respond to abiotic factor changes is necessary for predicting their population dynamics. In this study, we investigated the geographical distribution of the medically important blowfly, Achoetandrus rufifacies (Macquart) (Diptera: Calliphoridae), and ascertained the response to climatic and physio-environmental factors in Chiang Mai, northern Thailand. Adult fly surveys were carried out every 2 weeks from May 2009 to May 2010 at 18 systematically randomized study sites in three districts of Chiang Mai province (Mueang Chiang Mai, Mae Rim, and Hang Dong), using reconstructable funnel traps with 1-day tainted beef offal as bait. During the study period, 8,861 adult A. rufifacies were captured, with peak densities being observed at the end of winter (i.e., late February) and throughout most of the summer (May to March). Population density had a weak but significant (α = 0.05) positive correlation with temperature (r = 0.329) and light intensity (r = 0.231), and a weak but significant (α = 0.05) negative correlation with relative humidity (r = -0.236). From the six ecological land use types (disturbed mixed deciduous forest, mixed deciduous forest, mixed orchard, lowland village, city town, and paddy field), greater fly densities were observed generally in the disturbed mixed deciduous forest and lowland village, but not in the paddy fields. In conclusion, A. rufifacies are abundant from the end of winter and throughout most of the summer in northern Thailand, with population density being weakly positively correlated with temperature and light intensity, but weakly negatively correlated with relative humidity. The greatest densities of this fly species were collected in disturbed mixed deciduous forest and lowland village land uses. The prediction of annual and season specific distributions of A. rufifacies were provided in each season and all-year patterns using a co-kriging approach (ArcGIS9.2).

  11. Lembranças de aluna/professora numa escola criada por uma mineradora de carvão: memórias e significados

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    Giani Rabelo

    2012-07-01

    Full Text Available  O artigo apresenta percepções teóricas metodológicas sobre duas entrevistas que trazem as lembranças de uma aluna e também professora numa escola criada pela Carbonífera Próspera, em Criciúma, Sul de Santa Catarina, em 1946. Além de tratar da relação do trabalho com a educação numa perspectiva histórica, o referido estudo problematiza a memória no âmbito da história oral como fonte histórica de pesquisa.Palavras-chave: educação e trabalho, história da educação, história oral, memória.   Abstract The article presents methodologies theoretical perceptions on two interviews that bring the memory of a pupil and teacher in a school created Prospera Carboniferous in Criciúma, South of Santa Catarina, in 1946. Besides discut the relation of the work with the education in a historical perspective, the related study problematical the memory in the ambit of oral history as historical source of research. Keywords: education and work, history of the education, oral history, memory. 

  12. Calliphoridae (Diptera from wild, suburban, and urban sites at three Southeast Patagonian localities: Calliphoridae (Diptera de ambientes no habitados, suburbanos y urbanos en tres localidades del sudeste patagónico

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    Juan C. Mariluis

    2008-06-01

    Full Text Available Species composition, relative abundance, sex ratio and habitat preference of blowflies (Diptera: Calliphoridae from Caleta Olivia, Puerto Deseado, and Puerto San Julián (Santa Cruz Province, Argentina were studied during late spring and summer in 2004-2005. Results showed a higher prevalence of the exotic species, Calliphora vicina (Robineau-Desvoidy and Phaenicia sericata (Meigen at urban sites over the natives, Compsomyops fulvicrura (Robineau-Desvoidy and Sarconesia chlorogaster (Wiedemann, which shows a strong preference for those sampling sites either not inhabited or less influenced by human activities. Sex ratio was female biased for all species, except for Sarconesia chlorogaster, which exhibited a male prevalence over females.Durante fines de la primavera-verano de 2004-2005, se analizó la composición, abundancia relativa y proporción de sexos de especies de Calliphoridae (Diptera en las localidades de Caleta Olivia, Puerto Deseado y Puerto San Julián (Provincia de Santa Cruz, Argentina. Tres sitios de muestreo afectados diferencialmente por la intervención humana fueron seleccionados en cada localidad. En las áreas urbanas, las especies exóticas Calliphora vicina (Robineau-Desvoidy y Phaenicia sericata (Meigen fueron dominantes. Por el contrario, las nativas Compsomyiops fulvicrura (Robineau-Desvoidy y Sarconesia chlorogaster (Wiedemann predominaron tanto en áreas inhabitadas como en aquellas con bajo impacto humano. En los cebos utilizados para las capturas, se observó mayor proporción de hembras en todas las especies, excepto Sarconesia chlorogaster que mostró mayor proporción de machos.

  13. Características reprodutivas da paca fêmea (Agouti paca criada em cativeiro Reprodutive characteristics of the female paca (Agouti paca raised in captivity

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    Diva Anelie de Araújo Guimarães

    2008-01-01

    Full Text Available O objetivo do trabalho foi estudar a biologia reprodutiva da Agouti paca criada em cativeiro. Os resultados demonstraram que, a duração média do ciclo estral foi de 32,5 + 3,7 dias e o período gestacional de 148,6 + 4,8 dias. O intervalo entre partos foi de 224,5 + 52,2 dias e o primeiro cio pós-parto foi de 25,6 + 8,8 dias. A maioria (55,6 % das fêmeas apresentou dois partos por ano, com o nascimento de um filhote por parto, sendo 44,7 % fêmeas e 55,3 % machos. Ao nascer o peso médio das fêmeas foi de 605,9 + 87,5 g e dos machos 736,7 + 108,4 g (P The objective of this paper was to study the reproductive biology of the Agouti paca raised in captivity. It was observed that the estrous cycle average was 32.5 + 3.7 days, gestation length 148.6 + 4.8 days, parturition interval 224.5 + 52.2 days, and the first post-partum 25.6 + 8.8 days. It was also observed that 55.5% of the females presented two parturitions per year with one young per parturition, of which 44.7% were females and 55.3% were males. The birth weight was 605.9 + 87.8 g for females and 736.7 + 108.4 g for males (P<0.05. Puberty in females occurred between 8 to 12 months; for this, however, more detailed investigations are necessary.

  14. Alterações patológicas do sistema genital de cutias (Dasyprocta aguti Linnaeus, 1758 fêmeas criadas em cativeiro

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    Jael S. Batista

    Full Text Available Resumo: Este artigo apresenta relatos de sete distintas patologias de origem reprodutiva ainda não descritas em cutias (Dasyprocta aguti Linnaeus, 1758 fêmeas, que afetaram a fertilidade ou resultaram na morte do animal. Descreveu-se a natureza, a localização e a frequência das alterações patológicas macroscópicas e histológicas dos órgãos que compõem o sistema reprodutivo de cutias fêmeas, criadas sob condições de cativeiro no semiárido do Brasil. Foram avaliados através do exame anatomopatológico o aparelho reprodutivo de trinta e nove cutias mortas naturalmente e encaminhadas ao Laboratório de Patologia Veterinária, no período de fevereiro de 2010 a maio de 2015. Destas, constatou-se alterações patológicas no sistema reprodutivo de 10 (25,6 %. No total, 13 alterações foram observadas, sendo que, em alguns animais haviam a coexistência de mais de uma alteração. Assim, as alterações patológicas encontradas foram: endometrite (n=4; 30,8%, piometra (n=3; 23%, retenção de placenta (n=2; 15,4%, maceração fetal (n=1; 7,7%, mumificação fetal (n=1; 7,7%, parto distócico (n=1; 7,7% e ovários afuncionais (n=1; 7,7%.

  15. Sarcophagidae and Calliphoridae related to Rhinella schneideri (Anura, Bufonidae, Bothrops moojeni (Reptilia, Serpentes and Mabuya frenata (Reptilia, Lacertilia carcasses in Brasília, Brazil

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    Roger Maia Dias Ledo

    2012-09-01

    Full Text Available Sarcophagidae and Calliphoridae related to Rhinella schneideri (Anura, Bufonidae, Bothrops moojeni (Reptilia, Serpentes and Mabuya frenata (Reptilia, Lacertilia carcasses in Brasília, Brazil. This paper presents a list of necrophagous insects associated with small size carrions of two reptiles and one amphibian, found in areas of riparian forests and Cerrado sensu stricto physiognomies in a Conservation Unit located in Brasilia, Distrito Federal. We found seven species of insects related to these carcasses, being five Sarcophagidae, one Calliphoridae and one Braconidae parasitoid wasp. Lucilia eximia and Peckia (Pattonella intermutans were the most abundant species in the study, corroborating with other studies that suggests that these species have specializations for colonization of small size animal carcasses.

  16. Keys to the blow flies of Taiwan, with a checklist of recorded species and the description of a new species of Paradichosia Senior-White (Diptera, Calliphoridae

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    Shih-Tsai Yang

    2014-08-01

    Full Text Available Blow flies (Diptera: Calliphoridae show a great diversity in behavior and ecology, play important roles in ecosystems, and have medical and forensic importance to humans. Despite this, the taxonomy and classification of Taiwan's Calliphoridae have rarely been studied. In this study, specimens of Taiwanese calliphorids were collected and carefully studied, and all 76 species recorded in Taiwan are listed following the identification keys. Dichotomous keys to all subfamilies, tribes, genera, and species of blow flies recorded in Taiwan are provided, including 16 species that are newly recorded from Taiwan. In addition, one new species of the genus Paradichosia Senior-White is described and illustrated. We also discuss the morphological differences between the specimens of Silbomyia hoeneana Enderlein collected from China and Taiwan, a species that has only been found previously in Southern China.

  17. Brachymeria podagrica (Fabricius (Hymenoptera: Chalcididae as a parasitoid of Chrysomya albiceps (Wiedemann (Diptera: Calliphoridae: first report in Brazil

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    Marchiori C.H.

    2002-01-01

    Full Text Available Este trabalho relata a primeira ocorrência do parasitóide Brachymeria podagrica (Fabricius, 1787 parasitando estádios imaturos de Chrysomya albiceps (Wiedemann (Diptera: Calliphoridae no Brasil. Utilizaram-se armadilhas contendo como isca vísceras de galinha. As pupas foram obtidas pelo método de flutuação. Elas foram colocadas individualmente em cápsulas de gelatina e mantidas até a emergência das moscas e/ou dos parasitóides. Foram obtidas 29 pupas de C. albiceps, quatro das quais emergiram parasitóides. A prevalência de parasitismo foi de 13,7%.

  18. [Cutaneous myiasis by Cochliomyia hominivorax (Coquerel) (Diptera Calliphoridae) in Hospital Universidad del Norte, Soledad, Atlántico].

    Science.gov (United States)

    de la Ossa, Napoleón; Castro, Luis Eduardo; Visbal, Lila; Santos, Ana María; Díaz, Esther; Romero-Vivas, Claudia M E

    2009-03-01

    Human myiasis is the parasitism of human tissues by fly larvae. Diagnoses are based on clinical pattern of tissue damage and presence of insect stages. Herein, a case myiasis is described in a seven-year-old female child. She presented with fever associated with abscessed scalp lesions containing exposed larvae. Severe pediculosis was also observed. The patient was hospitalized and treated with clindamycin, gentamicin (for bacterial secondary infections) and ivermectin (treatment for lice) after which the patient showed clinical improvement and was discharged four days later. Since human myiasis can be caused by a number of different species, larvae were collected from the patient and identified as those of Cochliomyia hominivorax (Diptera: Calliphoridae). Because other cases of coinfestation of flies and lice are on record, health workers are to be alerted about the possible pediculosis-myasis risk.

  19. Effect of Lunar Phases, Tides, and Wind Speed on the Abundance of Diptera Calliphoridae in a Mangrove Swamp.

    Science.gov (United States)

    Batista-da-Silva, J A

    2014-02-01

    Abiotic factors, such as lunar phases and tides, have a significant effect on insect development. Reproduction and immature development are usually interlinked to these abiotic factors. The tide is at its highest levels at full moon or new moon, hindering the feeding of the immature or causing their drowning. The oviposition by adult females is also compromised on these days because much of the available food is submerged. Another important abiotic factor is the wind, which displaces odoriferous particles in the air. Wind speed and direction are important elements to indicate potential sources of food for insects. I report on the effects of lunar phases, tides, and wind speed on the Calliphoridae fauna in mangrove swamps. The different species collected were identified, and the predominant species in the area were quantified. A total of 1,710 flies were collected over a 1-year period. Six Calliphoridae flies, Chloroprocta idioidea (Robineau-Desvoidy), Chrysomya megacephala (Fabricius), Chrysomya albiceps (Wiedemann), Chrysomya putoria (Wiedemann), Cochliomyia macellaria (Fabricius), and Lucilia eximia (Wiedemann) were collected. Data indicated that lunar phases have a significant effect on the abundance of C. albiceps (r = 0.39, p < 0.01), and that the variation of the tides also affected the abundance of C. putoria (r = 0.40, p < 0.00), C. macellaria (r = 0.41, p < 0.00), and C. idioidea (r = 0.31, p < 0.04). The wind speed, however, did not affect these species.

  20. Biometria corporal e parâmetros hematológicos de Trachemys scripta elegans e Trachemys dorbignyi (Testudines: Emydidae criadas em cativeiro em Petrolina, Pernambuco

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    Adriana Gradela

    Full Text Available RESUMO: Este estudo objetivou avaliar a biometria corporal e o perfil hematológico de Trachemys scripta elegans (N=28 e de Trachemys dorbignyi (N=22 criadas em cativeiro na região do submédio do Vale do São Francisco, semiárido nordestino brasileiro, visando estabelecer valores sanguíneos básicos de saúde e gerar dados úteis na fisiologia comparativa de Testudines. Após 120 dias de adaptação e jejum de 24 horas, 2,5 mL de sangue foram coletados do seio occipital dorsal e depositados em tubo com heparina sódica para a avaliação, na sequência, dos níveis hematologicos. A contagem total de eritrócitos (CTE e global de leucócitos (CGL foi realizada em câmara de Neubauer; a dosagem de hemoglobina (HGB pelo método da método da cianometahemoglobina e o hematócrito (HCT através da técnica do microhematócrito. A partir da CTE estabeleceram-se matematicamente os índices hematimétricos. A biometria corporal também foi avaliada: a massa corporal (MC, g; b dimensões máximas da carapaça [comprimento (CMC, cm e largura (LMC, cm];c dimensões máximas do plastrão [comprimento (CMP, cm e largura (LMP, cm]; d comprimento total da cauda (CTC, cm; e comprimento linear da base da cauda ao orifício cloacal (CprC, cm; f comprimento linear do orifício cloacal a extremidade da cauda(CpoC, cm. T. scripta elegans apresentaram valores maiores (P 0,05 entre as espécies. Os resultados demostram que a maior parte da variação observada entre T. scripta elegans e T. dorbignyi é explicada pelas variáveis biométricas e que algumas correlações hematológicas caracterizam diferenças interespecíficas. Conclui-se que os resultados lançam luz sobre valores de referência para estas espécies mantidas em cativeiro na região do semiárido e servem como um modelo para a fisiologia comparativa intra e interespécies.

  1. Morfometría geométrica alar para la identificación de Chrysomya albiceps y C. megacephala (Diptera: Calliphoridae de Venezuela Geometric wing morphometrics for Chrysomya albiceps and C. megacephala identification (Diptera: Calliphoridae from Venezuela

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    Marianela Vásquez

    2012-09-01

    Full Text Available Calliphoridae es una de las familias con el mayor número de especies de importancia forense, donde sus fases inmaduras se alimentan y desarrollan sobre material en descomposición. En Venezuela, son pocos los estudios en este taxon, sin embargo se ha reportado la dominancia del género Chrysomya sobre los otros dípteros. El objetivo fue analizar la variación morfométrica en la arquitectura alar, como herramienta de apoyo a la identificación de dípteros de importancia forense. Se fotografiaron 168 alas de C. albiceps (n=111 y C. megacephala (n=57 y se registraron configuraciones de coordenadas (x, y, se alinearon mediante Análisis Generalizado de Procrustes. Se efectuaron Análisis de Componentes Principales y comparaciones pareadas entre distancia parcial de Procrustes. El análisis estadístico de varianza encontró diferencias en el tamaño isométrico del ala (Kruskal-Wallis. El ACP mostró la separación de ambas especies, y la prueba de configuraciones determinó diferencias significativas (F Goodall. Las principales diferencias entre ambas especies se encontraron en: ruptura de la subcosta, unión de R2+3 con el borde del ala, unión dm-cu, y unión de Cu con dm-cu, lo que confirma que la morfometría alar es una herramienta idónea en la discriminación de especies de Calliphoridae.Calliphoridae is one of the families with the greatest number of species with forensic importance, which immature stages feed and develop on decaying material. in Venezuela, there are few studies on this taxon reporting the dominance of Chrysomya over other carrion flies. The goal of this study was to analyze the variations on wing morphometrics, to support the identification of two forensic flies. For this we photographed a total of 168 wings from C. albiceps (n=111 and C. megacephala (n=57. Landmark coordinate (x, y configurations were registered and aligned by Generalized Procrustes Analysis. Principal Component Analysis and shape significance test

  2. Sobrevivência de pupas de Lucilia eximia (Wiedemann (Diptera, Calliphoridae após submersão em laboratório Survival of Lucilia eximia (Wiedemann (Diptera, Calliphoridae pupae after submersion in laboratory

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    Alex Sandro Barros de Souza

    2009-01-01

    Full Text Available Entre Maio e Junho de 2008 foi observado a sobrevivência das pupas de Lucilia eximia (Diptera, Calliphoridae após submersão em laboratório. Para este experimento foram utilizadas 480 pupas de mesma idade, sendo estas divididas em oito grupos: o controle não foi submerso e os demais grupos foram submersos de um a sete dias. O aumento do período de submersão diminui a sobrevivência, com um dia de submersão a sobrevivência é de 80%, com dois dias 40%, é de 30% a partir do terceiro dia, no quarto dia cai para 23,34% e no quinto dia fica em 10%. Após este período a mortalidade sobe para 100%. Este padrão pode ser explicado pela curva "U-shaped" que ocorre no consumo de O2 durante o período pupal, onde o consumo é maior no início e no final do período pupal. O tempo de submersão também afeta o tempo de desenvolvimento, aumentando o período pupal. Estes dados têm o potencial para serem utilizados em investigações envolvendo Entomologia Forense, para a estimativa do tempo de submersão de um cadáver.Between May and June of 2008, the survival of the pupae of Lucilia eximia (Diptera, Calliphoridae was observed after submersion in laboratory. For this experiment 480 pupae of same age were used, these were divided in eight groups: the group control was not submerged and the other groups were submerged from one to seven days. The increase of the submersion period reduces the survival, with one day of submersion the survival is of 80%, with two days 40%, of 30% with three days, in the fourth day it falls to 23,34% and in the fifth day it is 10%. After this period the mortality rises to 100%. This pattern can be explained by the curve "U-shaped" that happens in the consumption of O2 during the pupal period, where the consumption is larger at the beginning and at the end of the pupal period. The time of submersion also affects the development time, increasing the pupal period. These data are potentially useful in estimating duration of

  3. Perfil hematológico de cabras clinicamente sadias criadas no Cariri paraibano Profile hematological of goat clinical healthy servants in Cariri paraibano

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    Leilson Rocha Bezerra

    2008-06-01

    Full Text Available Objetivou-se com este trabalho estudar o perfil hematológico de cabras sem raça definida, criadas na região do Cariri paraibano em regime semi-intensivo e mantidas sob as mesmas condições ambientais e de manejo, como também a busca de valores de referência que se adeqüem à nossa região. Foram coletadas amostras sanguíneas de 143 animais clinicamente sadios, com idades entre 1 e 12 anos. A partir de uma única amostra de sangue de cada animal foram realizados o eritrograma (contagem de hemácias, volume globular, hemoglobina, volume corpuscular médio, hemoglobina corpuscular média e concentração de hemoglobina corpuscular média e o leucograma (contagem total e diferencial de leucócitos. Os dados obtidos foram avaliados estatisticamente por meio do Teste de Tukey em nível de significância de 5%, comparando os resultados com valores encontrados por outros autores. Os resultados demonstraram que não foram evidenciadas diferenças significativas de resultados encontrados por outros autores para as variáveis avaliadas, porém houve diferenças para alguns parâmetros descritos na literatura para animais de mesma raça e faixas etárias, refletindo a influência de fatores como altitude,clima, nutrição e manejo entre outros. Desta forma, evidencia-se a necessidade de pesquisas para o estabelecimento de valores regionais.On aimed to study the profile hematological of female goats of the sem race defined, raised in the area of Cariri Paraibano at a semi-intensive regime and under the same environmental and management conditions and handling as well as the search for reference values that adequate to our region. Sanguine samples of 143 clinically healthy animals were collected with ages between 1 and 12 years. Starting from just a single sample of blood of each animal the eritrograma was accomplished (Red blood cell count, packed cell volume, hemoglobin, mean corpuscular values, mean corpuscular hemoglobin and mean corpuscular

  4. Goniometria dos membros pélvicos de pacas (Cuniculus paca criadas em cativeiro Goniometry of the hind limbs of pacas (Cuniculus paca raised in captivity

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    Fábio André Pinheiro de Araújo

    2009-12-01

    Full Text Available O trabalho teve por objetivo avaliar por meio de estudos goniométricos os membros pélvicos de pacas criadas em cativeiro. Foram utilizadas oito pacas (Cuniculus paca, sendo três fêmeas e cinco machos, com peso entre 6,0 kg e 8,2 kg, nos quais foi aferido o movimento de extensão e flexão das articulações coxofemoral, joelho e tarsocrural utilizando-se um goniômetro de plástico. Foram realizadas duas aferições para cada membro pelo mesmo avaliador, com intervalo de uma semana entre cada aferição, com os animais sob anestesia dissociativa. Não houve diferença estatística nas aferições dos ângulos entres os momentos. As variações entre as medidas de flexão e extensão, efetuadas em cada momento pelo mesmo avaliador, foi entre 1º e 5º para cada membro. Os valores goniométricos médios foram: flexão de 43,96º±7,62 e extensão de 118,31º±9,79 para a articulação coxofemoral; flexão de 54,25º±10,24 e extensão 131,53º±7,89 para o joelho; flexão de 45,94º±7,16 e extensão de 145,03º±5,06 para a articulação tarsocrural. Sendo assim, observou-se que a amplitude de movimento das pacas é maior na articulação tarsocrural, seguida respectivamente do joelho e da articulação coxofemoral.The aim of this study was to evaluate by goniometry the hind limbs of pacas raised in captivity. Eight mature pacas (Cuniculus paca, three females and five males, weighing 6.0-8.2 kg, were used. The quantity of joint flexion and extension motion of the hip joint, stifle joint and tarsocrural joint was measured using a plastic standard goniometer. Under dissociative anesthesia, two measures were made for each member in each animal by the same observer at an interval of one week. There were no statistical differences of the quantity of joint motion between the moments. The flexion and extension angles varied from 1º to 5º in each moment for the same observer. The mean values of the angles of flexion and extension were, respectively

  5. Avaliação do impacto do programa de coleta seletiva de lixo na frequência de calliphoridae e muscidae em Tupã-SP / Evaluation of the impact of selective collection program in the frequency of Calliphoridae and Muscidae in Tupã, São Paulo (Brazil

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    Leonice Seolin Dias

    2014-04-01

    Full Text Available The present paper discusses the importance of analysing the frequency of flies of the Muscidae and Calliphoridae family, evaluating the impact of the implementation of urban garbage collection’s programs. It was examined the case study of the city Tupa – Sao Paulo’s State- which was divided in two distinct phases: phase 1 – between June and October of 2001, characterized by the presence of landfill and; phase 2 – from November of 2001 to May of 2002, characterized by the beginning of the implementation of selective collection and recycling and gradual dump grouding. The procedure for the capture of the insects was made with ten traps made with two-liter plastics bottles placed in houses and trees around the landfill. As an attractive, 400 mL of spill from sugar cane was used and replaced weekly. In both phases of the research was observed that the most frequent and constant family was the Calliphoridae. There was a positive correlation before the selective collection between the number of Calliphoridae and the rainfall (0,638 and the number of muscideos and the average temperature (0,605. After the collection program started, this correlation was not observed any more. To conclude, the program of selective collection implanted in the city of Tupa resulted in a decrease in the frequency of diptera. The analysis of the Callidoridae and Muscidae float demonstrated to be an efficient indicator of the environmental quality.

  6. MOSCAS SINANTROPICAS PERJUDICIALES, UN DESAFÍO ACTUAL: Musca domestica L. (MUSCIDAE Y Chrysomya megacephala (FABRICIUS (CALLIPHORIDAE

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    Priscila Maria da Silva Gomes

    2015-12-01

    Full Text Available The synanthropic animals of a particular locality corresponds to some species of wild native fauna that have adapted either temporary or permanently, the new environment modified by human action. Musca domestica and Chrysomya megacephala have a eusinantropico behavior, or their life cycle occurs in anthropized environment. So the article aims to contribute to the characterization of Musca domestica L. (Diptera: Muscidae and Chrysomya megacephala (Fabricius (Diptera: Calliphoridae among the main species of harmful synanthropic flies food health importance. The control populations of harmful synanthropic species requires knowledge of the bionomics of the species. The adoption of advertising campaigns, environmental and health education projects, citizenship, to the biological vector control should be focused on: food waste reduction; Promotion programs (and expansion of existing ones of basic separation of organic waste, inorganic and risk of domestic origin, commercial and various public and private service activities; Mapping of special areas of risk to public health, especially areas with most polluting activity by human excreta and secretions and domestic animals, organic waste and wastewater focuses exposed to open air, aimed at implementing a continuous system of hygiene and sanitation, integrating the activities of various sectors of society in these areas DOI: http://dx.doi.org/10.12957/sustinere.2015.20002

  7. First report of myiasis caused by Cochliomyia hominivorax (Diptera: Calliphoridae) in a diabetic foot ulcer patient in Argentina.

    Science.gov (United States)

    Olea, María Sofía; Centeno, Néstor; Aybar, Cecilia Adriana Veggiani; Ortega, Eugenia Silvana; Galante, Guillermina Begoña; Olea, Luis; Juri, María Julia Dantur

    2014-02-01

    Myiasis is usually caused by flies of the Calliphoridae family, and Cochliomyia hominivorax is the etiological agent most frequently found in myiasis. The first case of myiasis in a diabetic foot of a 54-year-old male patient in Argentina is reported. The patient attended the hospital of the capital city of Tucumán Province for a consultation concerning an ulcer in his right foot, where the larval specimens were found. The identification of the immature larvae was based on their morphological characters, such as the cylindrical, segmented, white yellow-coloured body and tracheas with strong pigmentation. The larvae were removed, and the patient was treated with antibiotics. The larvae were reared until the adults were obtained. The adults were identified by the setose basal vein in the upper surface of the wing, denuded lower surface of the wing, short and reduced palps, and parafrontalia with black hairs outside the front row of setae. The main factor that favoured the development of myiasis is due to diabetes, which caused a loss of sensibility in the limb that resulted in late consultation. Moreover, the poor personal hygiene attracted the flies, and the foul-smelling discharge from the wound favoured the female's oviposition. There is a need to implement a program for prevention of myiasis, in which the population is made aware not only of the importance of good personal hygiene and home sanitation but also of the degree of implication of flies in the occurrence and development of this disease.

  8. Identification of forensically important Chrysomya (Diptera: Calliphoridae) species using the second ribosomal internal transcribed spacer (ITS2).

    Science.gov (United States)

    Nelson, Leigh A; Wallman, James F; Dowton, Mark

    2008-05-20

    The identification of forensically important blowflies of the genus Chrysomya (Diptera: Calliphoridae) may be hampered by their close morphological similarities, especially as immatures. In contrast to most previous studies, the utility of a nuclear rather than mitochondrial genetic marker was investigated to solve this problem. The second internal transcribed spacer (ITS2) of ribosomal DNA (rDNA) was amplified and sequenced from all nine Chrysomya species known from Australia. Difficulties encountered with direct sequencing of ITS2 for Chrysomya flavifrons necessitated cloning prior to sequencing for this species, which revealed a low level (0-0.23%) of intraindividual variation. Five restriction enzymes (DraI, BsaXI, BciVI, AseI and HinfI) were identified that were able to differentiate most members of the genus by polymerase chain reaction (PCR) restriction fragment length polymorphism (PCR-RFLP). The PCR-RFLP analysis revealed characteristic restriction profiles for all species except the closely related species pairs Chrysomya latifrons+Chrysomya semimetallica and Chrysomya incisuralis+Chrysomya rufifacies. Ch. incisuralis and Ch. rufifacies were able to be separated using the size differences resulting from amplification of the entire ITS region. The lack of intraspecific ITS2 sequence variation among eight Ch. incisuralis specimens was verified by the identical restriction profiles generated from these specimens. A DNA-based approach, such as PCR-RFLP, has the capacity to be useful for the identification of forensic entomological evidence in cases where morphological characters are unreliable.

  9. Abundância relativa e sazonalidade de espécies de Chrysomya (Diptera: Calliphoridae) no Pantanal Sul-Mato-Grossense, Brasil.

    OpenAIRE

    2010-01-01

    As espécies do gênero Chrysomya Robineau-Desvoidy 1830 (Diptera: Calliphoridae) exercem papel importante na saúde pública por causarem miíases e por veicularem enteropatógenos. Este estudo visou estimar a abundância relativa e a sazonalidade das três espécies de Chrysomya (C. albiceps, C. putoria e C. megacephala) que ocorrem no Pantanal. Os dípteros foram capturados em quatro armadilhas orientadas pelo vento (Wind Oriented Trap - W.O.T.), iscadas com fígado bovino deteriorado. As armadilhas ...

  10. Determinação de idade cronológica de Calliphoridae (Diptera através da deposição pós-emergencial de quitina

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    Emygdio L. A. Monteiro Filho

    1989-01-01

    Full Text Available A method for chronological age determination based on mesothoracic post-phragma post-emergence deposition of chitin is used for Chrysomya putoria and Phaenicia cuprina (Diptera, Calliphoridae. The age of these species were obtained by the maximum period of deposition which were of 11-12 days.

  11. Associação da medroxiprogesterona ao protocolo Ovsynch para inseminação artificial em tempo fixo de búfalas cíclicas (Bubalus bubalis) criadas na Amazônia Oriental

    OpenAIRE

    Garcia, A. R.; NAHUM, B. de S.; LOURENÇO JÚNIOR, J. B.; COSTA, N. A. da; K.S Gonçalves; MIYASAKI, M. Y. A.; A.F.C. Andrade; ARRUDA, R. P.

    2008-01-01

    O presente estudo visou avaliar os efeitos da associação da medroxiprogesterona (análogo sintético da progesterona) ao protocolo Ovsynch sobre o crescimento folicular, a ovulação e a taxa de concepção de búfalas criadas na Amazônia Oriental (Tracuateua-PA). Vinte e sete fêmeas adultas (G1 n=14 e G2 n=13), cíclicas, sem bezerro ao pé e com ECC 3,5 foram submetidas a Ovsynch. Os animais do G2 receberam 60 mg de medroxiprogesterona entre D0 e D7 (D0=início do tratamento). A ultra-sonografia ovar...

  12. Muscidae, Sarcophagidae, Calliphoridae e Mesembrinellidae (Diptera da Estação Biológica de Santa Lúcia (Santa Teresa, Espírito Santo, Brasil

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    Leandro Silva Barbosa

    2014-01-01

    Full Text Available O primeiro registro de dípteros das famílias Muscidae, Sarcophagidae, Calliphoridae e Mesembrinellidae da Estação Biológica de Santa Lúcia (EBSL é apresentado e discutido, baseado em coletas realizadas no ano de 2007. Os exemplares foram capturados com o auxílio de armadilha adaptada de modelo proposto para lepidópteros, utilizando sardinha como isca e Malaise, além de coleta ativa com rede entomológica. Como resultado foram obtidos 1.253 espécimes, com destaque para as famílias Calliphoridae, Muscidae, Sarcophagidae e Mesembrinellidae. É discutida a dualidade gerada pela presença de espécies com baixo grau de sinantropia e outras altamente sinantrópicas, caracterizando a área de coleta como uma região de transição entre áreas rural e florestal. A observação de espécies sinantrópicas está provavelmente correlacionada ao efeito de borda. Os resultados evidenciam a importância da criação de uma zona de amortecimento no entorno da EBSL com intuito de minimizar os impactos gerados às espécies nativas.

  13. Evaluation of the internal transcribed spacer 2 (ITS2) as a molecular marker for phylogenetic inference using sequence and secondary structure information in blow flies (Diptera: Calliphoridae).

    Science.gov (United States)

    Marinho, M A T; Junqueira, A C M; Azeredo-Espin, A M L

    2011-09-01

    The internal transcribed spacer 2 (ITS2) is a small non-coding region located inside the nuclear ribosomal DNA cluster. ITS2 sequence variability is thought to be appropriate to differentiate species and for phylogenetic reconstructions analyses, which can be further improved if structural information is considered. We evaluated the potential of ITS2 as a molecular marker for phylogenetic inference in Calliphoridae (Diptera: Brachycera) using a broad range of inference methods and different substitution models, accounting or not for structural information. Sequence analyses revealed a hierarchically organized pattern of sequence variation and a small level of nucleotide substitution saturation. Intragenomic variation due to small sequence repeats was found mainly in the most variable domain (IV), but it has no significant impact on the phylogenetic signal at the species level. Inferred secondary structures revealed that GC pairs are more frequently found flanking bulges and loops regions in more conserved domains, thus ensuring structure stability. In the phylogenetic analyses, the use of substitution models accounting for structural information significantly improves phylogenetic inference in both neighbour-joining and Bayesian analyses, although the former provides limited resolution for dealing with highly divergent sequences. For Bayesian analyses, a significant improvement in likelihood was observed when considering structure information, although with small changes in topology and overall support, probably reflecting better evolutionary rates estimates. Based on these findings, ITS2 is a suitable molecular marker for phylogenetic analyses in Calliphoridae, at both species and generic level. © Springer Science+Business Media B.V. 2011

  14. Effect of morphine on the growth rate of Calliphora stygia (Fabricius) (Diptera: Calliphoridae) and possible implications for forensic entomology.

    Science.gov (United States)

    George, Kelly A; Archer, Melanie S; Green, Lauren M; Conlan, Xavier A; Toop, Tes

    2009-12-15

    Insect specimens collected from decomposing bodies enable forensic entomologists to estimate the minimum post-mortem interval (PMI). Drugs and toxins within a corpse may affect the development rate of insects that feed on them and it is vital to quantify these effects to accurately calculate minimum PMI. This study investigated the effects of morphine on growth rates of the native Australian blowfly, Calliphora stygia (Fabricius) (Diptera: Calliphoridae). Several morphine concentrations were incorporated into pet mince to simulate post-mortem concentrations in morphine, codeine and/or heroin-dosed corpses. There were four treatments for feeding larvae; T 1: control (no morphine); T 2: 2 microg/g morphine; T 3: 10 microg/g morphine; and T 4: 20 microg/g morphine. Ten replicates of 50 larvae were grown at 22 degrees C for each treatment and their development was compared at four comparison intervals; CI 1: 4-day-old larvae; CI 2: 7-day-old larvae; CI 3: pupae; and CI 4: adults. Length and width were measured for larvae and pupae, and costae and tibiae were measured for adults. Additionally, day of pupariation, day of adult eclosion, and survivorship were calculated for each replicate. The continued presence of morphine in meat was qualitatively verified using high-performance liquid chromatography with acidic potassium permanganate chemiluminescence detection. Growth rates of C. stygia fed on morphine-spiked mince did not differ significantly from those fed on control mince for any comparison interval or parameter measured. This suggests that C. stygia is a reliable model to use to accurately age a corpse containing morphine at any of the concentrations investigated.

  15. Sarcophagidae and Calliphoridae related to Rhinella schneideri (Anura, Bufonidae, Bothrops moojeni (Reptilia, Serpentes and Mabuya frenata (Reptilia, Lacertilia carcasses in Brasília, Brazil Sarcophagidae e Calliphoridae associados às carcaças de Rhinella schneideri (Anura, Bufonidae, Bothrops moojeni (Reptilia, Serpentes e Mabuya frenata (Reptilia, Lacertilia em Brasília, Distrito Federal, Brasil

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    Roger Maia Dias Ledo

    2012-09-01

    Full Text Available Sarcophagidae and Calliphoridae related to Rhinella schneideri (Anura, Bufonidae, Bothrops moojeni (Reptilia, Serpentes and Mabuya frenata (Reptilia, Lacertilia carcasses in Brasília, Brazil. This paper presents a list of necrophagous insects associated with small size carrions of two reptiles and one amphibian, found in areas of riparian forests and Cerrado sensu stricto physiognomies in a Conservation Unit located in Brasilia, Distrito Federal. We found seven species of insects related to these carcasses, being five Sarcophagidae, one Calliphoridae and one Braconidae parasitoid wasp. Lucilia eximia and Peckia (Pattonella intermutans were the most abundant species in the study, corroborating with other studies that suggests that these species have specializations for colonization of small size animal carcasses.Sarcophagidae e Calliphoridae associados às carcaças de Rhinella schneideri (Anura, Bufonidae, Bothrops moojeni (Reptilia, Serpentes e Mabuya frenata (Reptilia, Lacertilia em Brasília, Distrito Federal, Brasil. Este trabalho apresenta uma lista de insetos decompositores associados a carcaças de pequeno porte de dois répteis e de um anfíbio, encontrados em áreas de matas de galeria e de cerrado sensu stricto em unidades de conservação do Distrito Federal. Foram encontradas sete espécies de insetos associados a essas carcaças, sendo cinco sarcofagídeos, um califorídeo e uma vespa parasitóide Braconidae. Lucilia eximia e Peckia (Pattonella intermutans foram as espécies mais abundantes, corroborando com outros estudos que sugerem que estas espécies apresentam especializações para a colonização de carcaças menores.

  16. Abundância relativa e sazonalidade de espécies de Chrysomya (Diptera: Calliphoridae) no Pantanal Sul-Mato-Grossense, Brasil Chrysomya (Diptera: Calliphoridae) relative abundance and species seasonality in the Pantanal, State of Mato Grosso do Sul, Brazil

    OpenAIRE

    2010-01-01

    As espécies do gênero Chrysomya Robineau-Desvoidy 1830 (Diptera: Calliphoridae) exercem papel importante na saúde pública por causarem miíases e por veicularem enteropatógenos. Este estudo visou estimar a abundância relativa e a sazonalidade das três espécies de Chrysomya (C. albiceps, C. putoria e C. megacephala) que ocorrem no Pantanal. Os dípteros foram capturados em quatro armadilhas orientadas pelo vento (Wind Oriented Trap - W.O.T.), iscadas com fígado bovino deteriorado. As armadilhas ...

  17. Abundância relativa e sazonalidade de espécies de Chrysomya (Diptera: Calliphoridae no Pantanal Sul-Mato-Grossense, Brasil Chrysomya (Diptera: Calliphoridae relative abundance and species seasonality in the Pantanal, State of Mato Grosso do Sul, Brazil

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    Elaine Cristina Corrêa

    2010-06-01

    Full Text Available As espécies do gênero Chrysomya Robineau-Desvoidy 1830 (Diptera: Calliphoridae exercem papel importante na saúde pública por causarem miíases e por veicularem enteropatógenos. Este estudo visou estimar a abundância relativa e a sazonalidade das três espécies de Chrysomya (C. albiceps, C. putoria e C. megacephala que ocorrem no Pantanal. Os dípteros foram capturados em quatro armadilhas orientadas pelo vento (Wind Oriented Trap - W.O.T., iscadas com fígado bovino deteriorado. As armadilhas foram mantidas ativas durante todo o estudo, realizado na fazenda Nhumirim, base experimental da Embrapa Pantanal, sub-região da Nhecolândia, Pantanal Sul-Mato-Grossense. No período de dezembro/2004 a novembro/2007, foram capturados 159.086 espécimes de Calliphoridae, sendo 31,87% do gênero Chrysomya. A espécie mais abundante do gênero foi C. albiceps (30,86%, seguida de C. megacephala (0,67% e C. putoria (0,34%. As três espécies apresentaram flutuações sazonais semelhantes, com dois picos populacionais anuais, observados entre junho e agosto e entre outubro e dezembro.Species of the genus Chrysomya Robineau-Desvoidy 1830 (Diptera: Calliphoridae play an important role in public health due to myiasis and transmission of enteropathogens. This study aimed to estimate the relative abundance and seasonality of the three Chrysomya species (C. albiceps, C. putoria, and C. megacephala that occur in the Pantanal region. The flies were caught by four Wind Oriented Traps, baited with decayed bovine liver. Traps were kept active all over the study carried out from December 2004 to November 2007 at Nhumirim ranch, located at the Nhecolândia subregion, Southern Pantanal. A total of 159,086 Calliphoridae flies were collected and 31.87% of them belonged to the genus Chrysomya. Among calliphorid dipterans the most abundant species was C. albiceps (30.86%, followed by C. megacephala (0.67%, and C. putoria (0.34%. The three Chrysomya species showed similar

  18. Ocorrência e Sazonalidade de Muscóides (Diptera, Calliphoridae de Importância Sanitária no Município de Itaboraí, RJ, Brasil

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    José Batista-da-Silva

    2010-04-01

    Abstract. This work was carried out to contribute to the knowledge of Calliphoridae flies (Diptera in Itaboraí, RJ, Brazil and quantify the predominant species of health importance. The flies were captured in eight different points in the city over a one year period, always using fish as bait, separated by species and kept properly in an entomological box in the Laboratório de Transmissores de Leishmaniose (Setor de Entomologia Médica e Forense - IOC / FIOCRUZ, RJ. A total of 1792 Calliphoridae flies were captured, belonging to seven (7 species: Chloroprocta idioidea (Robineau-Desvoidy (0.11%, Chrysomya megacephala (Fabricius (87.94%, Chrysomya albiceps (Wiedemann (6.70%, Chrysomya putoria (Wiedemann (1.23%, Cochliomyia macellaria (Fabricius (0.56%, Hemilucilia segmentaria (Fabricius (0.33%, Lucilia eximia (Wiedemann (3.13%.

  19. Miíases Humanas Causadas por Larvas de Cochliomyia hominivorax (Coquerel (Diptera: Calliphoridae em São Gonçalo, RJ, Brasil: Uma Abordagem Sócio-Econômica

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    José Batista-da-Silva

    2011-12-01

    Human Myiasis Caused by Larvae of Cochliomyia hominivorax (Coquerel (Diptera: Calliphoridae in São Gonçalo, RJ, Brazil: Socio-Economic Approach Abstract. This study was carried out between April and September 2008 and reports on the occurrence of human myiasis caused by the New World screwworm, Cochliomyia hominivorax (Coquerel (Diptera: Calliphoridae in São Gonçalo in the state of Rio de Janeiro, Brazil. Liquid or solid vaseline was used to suffocate the larvae, which were then preserved in 70% ethanol and sent to the Instituto Oswaldo Cruz for identification. There were 01 male and 02 females black patients with ages ranging from 36 to 76 and C. hominivorax were identified in all 03 cases of myiasis. Open wounds were the main cause of the parasitosis, whereas poor personal hygiene, the low educational level, alcoholism, bedridden patients were possibly secondary factors.

  20. Aspectos fisiológicos de Chrysomya megacephala (F.) (Diptera: Calliphoridae): metabolismo energético, termorregulação e neurofisiologia

    OpenAIRE

    Gomes, Guilherme

    2012-01-01

    Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) foi introduzida no Brasil há algumas décadas atrás, e é uma espécie de mosca-varejeira de considerável importância médico-sanitária por ser veiculadora de enteropatógenos, poder causar miíases secundárias, e também ter grande importância em estudos forenses por auxiliar na estimativa do intervalo pós-morte (IPM) em cadáveres, além de ter importância agrícola, como polinizadora. Desta forma, diversos trabalhos utilizam esta espécie com...

  1. Traumatic Myiasis Caused by an Association of Sarcophaga tibialis (Diptera: Sarcophagidae) and Lucilia sericata (Diptera: Calliphoridae) in a Domestic Cat in Italy.

    Science.gov (United States)

    Pezzi, Marco; Whitmore, Daniel; Chicca, Milvia; Lanfredi, Margherita; Leis, Marilena

    2015-08-01

    We describe here a rare case of traumatic myiasis occurred in August 2014, caused by an association of 2 Diptera species, Sarcophaga tibialis Macquart (Diptera: Sarcophagidae) and Lucilia sericata (Meigen) (Diptera: Calliphoridae), in a domestic cat in northern Italy. Species identification was based on adult male morphology. The present case is the first report of S. tibialis as an agent of myiasis in Italy, and also the first ever report of myiasis caused by an association of S. tibialis and L. sericata. The cat developed an extensive traumatic myiasis in a large wound on the rump, which was treated pharmacologically and surgically. The biology, ecology, and distribution of S. tibialis and L. sericata are also discussed. A literature review is provided on cases of myiasis caused by S. tibialis, and cases of myiasis by L. sericata involving cats worldwide and humans and animals in Italy.

  2. Effects of forest fragmentation on dipterofauna (Calliphoridae at the Reserva Biológica do Tinguá, Nova Iguaçu, RJ

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    ACP. Ferraz

    Full Text Available Samples were collected every month in three different sites of the Reserva Biológica do Tinguá, Brazil: site A was located on the border of the forest and sites B and C were located 1,000 and 500 m, respectively, towards the forest interior. The objective was to determine edge effects on a fragment of the Atlantic Forest. The greatest species richness was observed in sites A and B (23 species, compared with site C (16 species. Site A showed the greatest abundance and constancy, independent of the degree of synanthropy. Asynanthropic species were more abundant and constant in sites B and C. Site B showed the greatest diversity; and sites A and B showed the greatest similarity of populations. There was no significant correlation between Calliphoridae richness and canopy openness except in site C. Richness and abundance were positively correlated with subwood density, except for richness in site B.

  3. Correlation of molecular expression with diel rhythm of oviposition in Calliphora vicina (Robineau-Desvoidy) (Diptera: Calliphoridae) and implications for forensic entomology.

    Science.gov (United States)

    George, Kelly A; Archer, Melanie S; Toop, Tes

    2015-01-01

    This study explored the molecular mechanisms potentially underlying blow fly nocturnal oviposition. A behavioral study revealed that Calliphora vicina (Robineau-Desvoidy) (Diptera: Calliphoridae) possesses a diel rhythm of oviposition in light under 12:12 light/dark conditions. Reversal to 12:12 dark/light resulted in oviposition behavior changing to align with the adjusted regime in most females, but four of 59 experimental females lacked a diel rhythm of oviposition (were arrhythmic). Real-time PCR was used to monitor the molecular expression levels of known circadian genes per and tim in C. vicina to determine whether gene expression and behavior correlated. As with behavior, reversing light/dark conditions changed rhythmic gene expression to align with an adjusted light regime. This suggests that although it is unlikely that C. vicina will colonize dead bodies at night, arrhythmic females and oviposition in the dark was demonstrated.

  4. Scanning electron microscopic studies on antenna of Hemipyrellia ligurriens (Wiedemann, 1830) (Diptera: Calliphoridae)-A blow fly species of forensic importance.

    Science.gov (United States)

    Hore, Garima; Maity, Aniruddha; Naskar, Atanu; Ansar, Waliza; Ghosh, Shyamasree; Saha, Goutam Kumar; Banerjee, Dhriti

    2017-08-01

    Blow flies (Diptera: Calliphoridae) are one of the foremost organisms amongst forensic insects to colonize corpses shortly after death, thus are of immense importance in the domain of forensic entomology. The blow fly Hemipyrellia ligurriens (Wiedemann, 1830) (Diptera: Calliphoridae) is considered as a forensically important fly species globally and is also known for its medical and veterinary importance. In the present study, we report for the first time scanning electron microscopic studies on the morphology of sensilla of antenna of adult male and female of H. ligurriens is with profound importance in better understanding of the insect morphology from forensic entomological perspective, and also could aid in proper identification of the species from other calliphorid flies. The structural peculiarities observed in the (i) antenna of H. ligurriens with three segments- scape, pedicel and flagellum with dorso-laterally placed arista (ii) densely covered microtrichia and most abundant trichoid sensilla identified on the antenna (iii) observation of only one type of sensilla, chaetic sensilla (ChI) on the scape (iv) two types of chaetic sensilla (ChI and ChII) and styloconic sensilla on the pedicel (v) the flagellum with three types of sensilla- trichoid, basiconic and coeloconic sensilla (vi) Basiconic sensilla with multiporous surfaces with characteristic olfactory function. Moderate sexual dimorphism in the width of the flagellum, the females with wider flagella than the males, bear significance to the fact that they bear more multi-porous sensilla than the males, thus suffice their need to detect oviposition sites. Significant difference was observed in the length and width of coeloconic sensilla between the two sexes, the females showed bigger coeloconic sensilla, suggesting their function in oviposition site detection and successful colonization in corpses. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. Effects on microhymenopteran progeny of different host exposure periods (Chrysomya megacephala, Calliphoridae to the parasitoid wasp Nasonia vitripennis (Hymenoptera: Pteromalidae

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    Renata da Silva Mello

    2010-02-01

    Full Text Available To test the assumption that exposure of the host to parasitoid for long periods could provoke superparasitism, the aim of this work was to test the consequences on the immature development time, productivity of parasitoids per pupa, sex ratio and rate of parasitism of Nasonia vitripennis bred in Chrysomya megacephala pupae. Each individual pupa was placed in a glass tube with one parasitoid female for 24, 48, 72 and 96 h period of exposure, under controlled laboratory conditions. Twenty replicates of each period were performed. ANOVA with a 5% significance level was applied. The 72 h exposure caused the immature development time to increase. The mean parasitoids emergence per pupa did not vary significantly with the time of exposure. There were a significantly higher number of females than males and a trend in sex ratio deviation towards the females in all of the treatments. An increase in the number of unviable pupae rates were observed with increased exposure time.Para testar a hipótese que longos períodos de exposição do hospedeiro ao parasitóide podem ocasionar o superparasitismo, o presente estudo teve como objetivo testar as conseqüências sobre o tempo de desenvolvimento do imaturo, a produtividade de parasitóides, razão sexual e taxa de parasitismo de Nasonia vitripennis criadas em pupas de Chrysomya megacephala. Cada pupa foi individualizada e alocada em um tudo de ensaio com uma fêmea de N. vitripennis por 24, 48, 72 ou 96 h, sob condições laboratoriais controladas. Foram realizadas 20 réplicas para cada período de exposição. ANOVA com significância de 5 % foi aplicada. A exposição de 72 h causou um aumento no tempo desenvolvimento. A média de emergência de parasitóides não variou significativamente entre os tempos de exposição. Houve um número significativamente maior de fêmeas e uma tendência ao desvio da razão sexual para fêmeas em todos os tratamentos. Foi verificado um aumento no número de pupas invi

  6. Ocorrência de ectoparasitos em avestruzes (Struthio camelus criadas no semi-árido baiano Occurence of ectoparasites in ostriches (Struthio camelus reared in semi-arid region of Bahia

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    Maria Ângela O. Almeida

    2008-09-01

    Full Text Available No período de maio a agosto de 2005, foram visitados 19 planteis de avestruzes na região semi-árida do município de Irecê, Bahia, objetivando identificar os ectoparasitos. As aves, predominantemente da raça "African Black", eram criadas em sistema intensivo e distribuídas, por faixa etária, em colônias, trio, casal ou creche. Durante a inspeção das aves, as plumas colhidas aleatoriamente, de várias regiões do corpo, foram acondicionadas em sacos plásticos e as larvas, colhidas das miíases, em álcool etílico a 70%. Em 74% (14/19 e 47% (04/19 dos plantéis encontravam-se avestruzes infestadas, respectivamente, pelas espécies Struthiopterolichus bicaudatus e Struthiolipeurus rheae. Somente em um plantel (5,3% foi encontrado um animal parasitado por larvas de Cochliomyia hominivorax.In the period of May to August of 2005, 19 ostrich's farms were visited in the semi-arid area of the Irecê, Bahia, in order to describe the ectoparasites. The African Black ostriches were reared in intensive system, and distributed for age group, in colonies, trio, couple or crèche. In each farm, after visually inspection of the ostrich, the feathers were taken in several areas of the body and were kept in plastic bags. The screwworms found were collected and stored in 70% ethanol. In 74% (14/19 and 47% (04/19 of the flocks were found ostriches infested, respectively, for the Struthiopterolichus bicaudatus (acari and Struthiolipeurus rheae (mallophaga. In only one flock (5.3% it was found an animal with Cochliomyia hominivorax larvae.

  7. Valores hematológicos de capivaras (Hydrochoerus Hydrochaeris criadas em cativeiro no município de Botucatu, SP Hematological values of captive capybara (Hydrochoerus Hydrochaeris in Botucatu county, São paulo, SP, Brazil

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    Maid Eliane Arouca

    2000-10-01

    Full Text Available Utilizaram-se onze capivaras (Hydrochoerus hydrochaeris, cinco machos e seis fêmeas, sadias, com dois anos de idade, criadas em regime de cativeiro na Fazenda Experimental Lageado da FMVZ - UNESP de Botucatu - SP, submetidas à anestesia dissociativa com midazolam e quetamina, para o estabelecimento de valores hematológicos. Este estudo compreendeu a determinação dos valores normais de eritrograma, leucograma, proteína plasmática total e fibrinogênio.Os valores médios encontrados para machos e fêmeas foram, respectivamente, 3,62 e 3,71x10(6/mil para eritrócitos, 15,0 e 15,4g/dl para hemoglobina, 48 e 49% para volume globular, 4630 e 5200/mil para leucócitos, 6,3 e 6,4g/dl para proteína plasmática total e 240 e 316,6mg/dl para fibrinogênio. Observou-se, desse modo, a ausência de diferenças entre os sexos.Eleven healthy capybara (Hydrochoerus hydrochaeris from the Experimental Farm Lageado - FMVZ - UNESP - Botucatu, SP - Brazil, were used in order to determine the hematological values, under Midazolan and Ketamine anesthesia. This study included the determination of normal eritrogram, leucogram, total plasmatic protein and fibrinogen values. The mean values for males and females were red cell 3.62 and 3.71x10(6/mul, hemoglobin 15.0 and 15.4g/dl, PCV 48 and 49%, white cell 4630 and 5200/mul, total plasmatic protein 6.3 and 6.4mg/dl and fibrinogen 240 and 316.6mg/dl, respectively. Diference among sex was not found.

  8. Enterobactérias associadas a adultos de Musca domestica (Linnaeus, 1758 (Diptera: Muscidae e Chrysomya megacephala (Fabricius, 1754 (Diptera: Calliphoridae no Jardim Zoológico, Rio de Janeiro Enterobacteria associated to adults of Musca domestica (Linnaeus, 1758 (Diptera: Muscidae and Chrysomya megacephala (Fabricius, 1754 (Diptera: Calliphoridae at the Zoo of Rio de Janeiro, Brazil

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    V.C. Oliveira

    2006-08-01

    Full Text Available Enterobactérias foram identificadas em adultos de Musca domestica (Linnaeus, 1758 (Diptera: Muscidae e Chrysomya megacephala (Fabricius, 1754 (Diptera: Calliphoridae. Ambas as espécies foram capturadas no Jardim Zoológico da cidade do Rio de Janeiro e tiveram a superfície externa do corpo lavada e o sistema digestivo dissecado, para análise bacteriológica. Identificaram-se Escherichia coli, Citrobacter sp., Proteus mirabilis, Morganella sp., Klebsiella sp., Pseudomonas sp., Enterobacter sp. e Salmonella Agona. P. mirabilis foi o isolado bacteriano mais freqüente. Em duas amostragens (8% de C. megacephala, isolou-se Salmonella Agona. As amostras de E. coli não foram enteropatogênicas. M. domestica e C. megacephala são potenciais veiculadoras de bactérias causadoras de enterites em humanos e animais.Enterobacteria were identified in adults of Musca domestica (Linnaeus, 1758 (Diptera: Muscidae and Chrysomya megacephala (Fabricius, 1754 (Diptera: Calliphoridae. Both species were captured in the Zoo of Rio de Janeiro. They had their external body surface washed and their digestive system dissected for bacteriological analysis. Escherichia coli, Citrobacter sp., Proteus mirabilis, Morganella sp., Klebsiella sp., Pseudomonas sp., Enterobacter sp. and Salmonella serovar Agona were isolated in the samples. P. mirabilis was the species most frequent isolated. Strains of Salmonella Agona were isolated from two samples (8% of C. megacephala. Enteropathogenic E. coli was not isolated. M. domestica and C. megacephala showed themselves as potential vectors of agents related to enteric diseases in humans and other animals.

  9. Forensically Important Blow Flies Chrysomya pinguis, C. villeneuvi, and Lucilia porphyrina (Diptera: Calliphoridae) in a Case of Human Remains in Thailand.

    Science.gov (United States)

    Monum, Tawatchai; Sukontason, Kabkaew L; Sribanditmongkol, Pongruk; Sukontason, Kom; Samerjai, Chutharat; Limsopatham, Kwankamol; Suwannayod, Suttida; Klong-Klaew, Tunwadee; Wannasan, Anchalee

    2017-02-01

    This is the first study to report Chrysomya pinguis (Walker) and Lucilia porphyrina (Walker) (Diptera: Calliphoridae) as forensically important blow fly species from human cadavers in Thailand, in addition to Chrysomya villeneuvi (Patton) already known in Thailand. In 2016, a fully decomposed body of an unknown adult male was discovered in a high mountainous forest during winter in Chiang Mai province. The remains were infested heavily with thousands of blow fly larvae feeding simultaneously on them. Morphological identification of adults reared from the larvae, and molecular analysis based on sequencing of 1,247 bp partial mitochondrial cytochrome c oxidase subunit 1 gene (CO1) of the larvae and puparia, confirmed the above mentioned 3 species. The approving forensic fly evidence by molecular approach was described for the first time in Thailand. Moreover, neighbor-joining phylogenetic analysis of the CO1 was performed to compare the relatedness of the species, thereby affirming the accuracy of identification. As species of entomofauna varies among cases in different geographic and climatic circumstances, C. pinguis and L. porphyrina were added to the list of Thai forensic entomology caseworks, including colonizers of human remains in open, high mountainous areas during winter. Further research should focus on these 3 species, for which no developmental data are currently available.

  10. The forensically important blow fly, Chrysomya megacephala (Diptera: Calliphoridae), is more likely to walk than fly to carrion at low light levels.

    Science.gov (United States)

    Smith, Joshua L; Palermo, Nicholas A; Theobald, Jamie C; Wells, Jeffrey D

    2016-09-01

    One factor that influences estimates of time since death using entomological evidence is whether or not blow flies nocturnally oviposit. Field studies focusing on egg laying have found it occurs on an inconsistent basis. A key but poorly understood factor in nocturnal oviposition is a blow fly's ability to locate carrion under low light levels. It has been speculated that blow flies are more likely to walk than fly to carrion during the night, but this has not been empirically tested. We directly compared guided walking versus flying using infrared sensors under low light levels in laboratory conditions for Chrysomya megacephala (F.) (Diptera: Calliphoridae), a blow fly previously described to be nocturnal. We found C. megacephala is more likely to walk than fly toward carrion under low light levels (p=0.016). We did not, however, find differences between males and females for walking (p=0.48) or flying (p=0.42) despite male C. megacephala possessing eyes better suited for increased light capture. These results demonstrate the need to better understand where blow flies go at night, as bodies found within a fly's walking distance are more likely to be colonized.

  11. Chrysomya albiceps (Wiedemann and Hemilucilia segmentaria (Fabricius (Diptera, Calliphoridae used to estimate the postmortem interval in a forensic case in Minas Gerais, Brazil

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    Cecília Kosmann

    2011-12-01

    Full Text Available ABSTRACT. Chrysomya albiceps (Wiedemann and Hemilucilia segmentaria (Fabricius (Diptera, Calliphoridae used to estimate the postmortem interval in a forensic case in Minas Gerais, Brazil. The corpse of a man was found in a Brazilian highland savanna (cerrado in the state of Minas Gerais. Fly larvae were collected at the crime scene and arrived at the laboratory three days afterwards. From the eight pre-pupae, seven adults of Chrysomya albiceps (Wiedemann, 1819 emerged and, from the two larvae, two adults of Hemilucilia segmentaria (Fabricius, 1805 were obtained. As necrophagous insects use corpses as a feeding resource, their development rate can be used as a tool to estimate the postmortem interval. The post-embryonary development stage of the immature collected on the body was estimated as the difference between the total development time and the time required for them to become adults in the lab. The estimated age of the maggots from both species and the minimum postmortem interval were four days. This is the first time that H. segmentaria is used to estimate the postmortem interval in a forensic case.

  12. Detection of food source by PCR analysis of the gut contents of Aldrichina grahami (Aldrich) (Diptera: Calliphoridae) during post-feeding period

    Institute of Scientific and Technical Information of China (English)

    KAI LI; GONG-YIN YE; JIA-YING ZHU; CUI HU

    2007-01-01

    The last meal of sarcophagous maggots may be useful in identifying the specieson whose flesh they have fed (the"host"species). The DNA profile of the host species mayindeed be detectable in the"last meal". In this paper, mitochondrial DNA analysis of gutcontents was used to identify the prior host of post-feeding larvae of Aldrichina grahami(Aldrich) (Diptera: Calliphoridae). A modified logistic equation was fitted to estimate theprobability of identifying the host under five different constant temperatures (16, 20, 24, 28and 32℃). Our results shows that the detected time ranged from a maximum of 24 h at 32℃to 42 h at 16℃ and a minimum of 12 h at 32℃ to 30 h at 16℃. Furthermore, the host detectiontime was also calculated to give the maximal time after larval hatching from the egg. Theseresults indicate that, in criminal cases where the maggots stray from the corpse, the last mealof the larvae should not be overlooked as potentially critical evidence.

  13. Efeito da escopolamina sobre o desenvolvimento de Chrysomya putoria (Diptera: Calliphoridae e sua importância para a estimativa do intervalo pós-morte

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    Patricia Jacqueline Thyssen

    2011-09-01

    Full Text Available A presença de drogas nos tecidos de um corpo pode afetar o desenvolvimento de larvas de moscas necrófagas que são encontradas alimentando-se neste substrato. Essa observação já foi constatada para várias drogas tais como heroína, cocaína, certos analgésicos, e compostos anfetamínicos e benzodiazepínicos, entre outras. Assim, neste estudo investigamos o efeito da escopolamina – uma droga amplamente usada como analgésico – sobre a taxa de desenvolvimento de larvas de Chrysomya putoria (Diptera: Calliphoridae, visando também avaliar e quantificar as conseqüências de tal interferência para a estimativa do intervalo pós-morte (IPM. Os resultados aqui obtidos mostram claramente que a escopolamina afetou significativamente a taxa de desenvolvimento de imaturos de C. putoria, quando presente em seu substrato alimentar. Tendo em vista que o IPM em muitos casos é calculado levando em consideração o peso ou a idade dos imaturos coletados em um corpo, a não consideração deste fator de interferência negativa pode conseqüentemente levar a uma subestimativa do tempo de morte em até 48 horas, em certos casos.

  14. Morfometria geométrica alar como ferramenta para a identificação de Lucilia sericata e Calliphora vicina (Diptera: Calliphoridae

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    Marcos Patrício Macedo

    2016-11-01

    Full Text Available Lucilia sericata (Meigen e Calliphora vicina (Robineau-Desvoidy (Diptera, Calliphoridae estão entre as espécies de dípteros de interesse forense. A correta identificação do espécime, ou fragmento deste, coletado em um local de crime é etapa fundamental para o emprego da entomologia forense em investigações criminais. Nesse estudo, avaliou-se a possibilidade de discriminação entre duas espécies de califorídeos pela morfologia alar, por meio de análises de morfometria geométrica. Foram analisadas as asas esquerdas de 253 espécimes, sendo 119 indivíduos de C. vicina e 134 de L. sericata, por meio de análises de variáveis canônicas e análises discriminantes. Das 253 comparações par a par, 2 erros de identificação (0,7% foram registrados para análise discriminante, enquanto 3 erros de classificação (2,3% foram registrados para o teste de validação cruzada. Esse resultado sugere um alto índice de confiabilidade da técnica. Mais estudos são necessários para a validação dessa técnica para seu uso na prática forense.

  15. Forensically Important Blow Flies Chrysomya pinguis, C. villeneuvi, and Lucilia porphyrina (Diptera: Calliphoridae) in a Case of Human Remains in Thailand

    Science.gov (United States)

    Monum, Tawatchai; Sukontason, Kabkaew L.; Sribanditmongkol, Pongruk; Sukontason, Kom; Samerjai, Chutharat; Limsopatham, Kwankamol; Suwannayod, Suttida; Klong-klaew, Tunwadee; Wannasan, Anchalee

    2017-01-01

    This is the first study to report Chrysomya pinguis (Walker) and Lucilia porphyrina (Walker) (Diptera: Calliphoridae) as forensically important blow fly species from human cadavers in Thailand, in addition to Chrysomya villeneuvi (Patton) already known in Thailand. In 2016, a fully decomposed body of an unknown adult male was discovered in a high mountainous forest during winter in Chiang Mai province. The remains were infested heavily with thousands of blow fly larvae feeding simultaneously on them. Morphological identification of adults reared from the larvae, and molecular analysis based on sequencing of 1,247 bp partial mitochondrial cytochrome c oxidase subunit 1 gene (CO1) of the larvae and puparia, confirmed the above mentioned 3 species. The approving forensic fly evidence by molecular approach was described for the first time in Thailand. Moreover, neighbor-joining phylogenetic analysis of the CO1 was performed to compare the relatedness of the species, thereby affirming the accuracy of identification. As species of entomofauna varies among cases in different geographic and climatic circumstances, C. pinguis and L. porphyrina were added to the list of Thai forensic entomology caseworks, including colonizers of human remains in open, high mountainous areas during winter. Further research should focus on these 3 species, for which no developmental data are currently available. PMID:28285509

  16. Use of wing morphometrics to identify populations of the Old World screwworm fly, Chrysomya bezziana (Diptera: Calliphoridae): a preliminary study of the utility of museum specimens.

    Science.gov (United States)

    Hall, M J R; MacLeod, N; Wardhana, A H

    2014-10-01

    The Old World screwworm (OWS) fly, Chrysomya bezziana (Diptera: Calliphoridae), is a major economic and welfare problem for humans and animals in the Old World tropics. Using a bootstrapped log likelihood ratio test of the output of Procrustes principal components and canonical variates analyses for a small sample of museum specimens from which 19 2D wing landmarks had been collected: (1) a consistent and statistically significant difference exists between landmark configurations derived from wings of pinned specimens and those removed from the body and mounted on slides; (2) a highly statistically significant sexual dimorphism in wing morphometry was identified; and (3) a highly statistically significant difference in wing morphometry between populations of the OWS fly from Africa (Tanzania, South Africa Sudan, Zaire, Zimbabwe,) and Asia (Sumba, Indonesia) exists. These results show that wing orientation and gender must be considered when conducting morphometric investigations of OWS fly wings. The latter result is also consistent with results from previous molecular and morphological studies, which indicate there are two distinct genetic lineages within this species. Wing morphometry holds great promise as a practical tool to aid in identification of the geographical origin of introductions of this important pest species, by providing diagnostic markers to distinguish geographical populations and complement molecular diagnostics.

  17. Miasis cutánea por Cochliomyia hominivorax (Coquerel (Díptera: Calliphoridae en el Hospital Universidad del Norte, Soledad, Atlántico

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    Claudia M.E. Romero-Vivas

    2009-03-01

    Full Text Available La miasis humana es el parasitismo de órganos y tejidos producido por especies de larvas del orden Díptera. El diagnóstico se realiza con base en hallazgos clínicos y se confirma con estudios entomológicos. Se presenta el caso de una niña de siete años de edad que fue llevada por su padre al servicio de urgencias por presentar fiebre asociada a una lesión abscedada en el cuero cabelludo, con salida espontánea de larvas. Como hallazgo en el examen físico se reportó pediculosis grave. La paciente fue hospitalizada y tratada con clindamicina, gentamicina e ivermectina, y mostró mejoría de sus condiciones clínicas. Se dio alta médica al cuarto día de estancia hospitalaria. Se recolectaron larvas en estadio dos de Cochliomyia hominivorax (Diptera: Calliphoridae directamente del área lesionada, observándose la asociación miasis-pediculosis; por lo tanto, se alerta a los trabajadores del área de la salud del riesgo potencial que representa la pediculosis para el desarrollo de la miasis.

  18. Hemilucilia segmentaria (Fabricius, 1805 (Diptera: Calliphoridae as new biological vector of eggs of Dermatobia hominis (Linnaeus Jr., 1781 (Diptera: Oestridae in Reserva Biológica do Tinguá, Rio de Janeiro, Brazil

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    Marinho Clarissa Rezende

    2003-01-01

    Full Text Available The aim of this note was to record for the first time the finding of Hemilucilia segmentaria acting as biological vector of Dermatobia hominis, during a study of the diversity of Calliphoridae at Reserva Biológica do Tinguá, Rio de Janeiro, Brazil. The insects were captured using traps baited with chicken vicera, for a period of 28-30 h twice per month. In the period of one year, 1987 insects were collected, 7.5% of which belonged to the H. segmentaria; of these a female was captured in May 2001, carrying a mass of 20 eggs on the left side of its abdomen.

  19. Desempenho reprodutivo de Nasonia vitripennis Walker (Hymenoptera: Pteromalidae em pupas crioconservadas de Chrysomya megacephala Fabricius (Diptera: Calliphoridae: avaliação preliminar Reproductive performance of Nasonia vitripennis Walker (Hymenoptera: Pteromalidae on Chrysomya megacephala Fabricius (Diptera: Calliphoridae cryopreserved pupae: preliminary evaluation

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    Eliane Maria Vieira Milward-de-Azevedo

    2004-02-01

    Full Text Available Avaliou-se o desempenho reprodutivo de Nasonia vitripennis Walker (Hymenoptera: Pteromalidae em pupas de Chrysomya megacephala Fabricius (Diptera: Calliphoridae previamente armazenadas a - 20ºC de temperatura, durante 77 dias, com e sem passagem prévia em nitrogênio líquido (NL por um, três e 15 minutos. O grupo controle foi caracterizado por pupas hospedeiras frescas. Os muscóides foram expostos aos parasitóides durante 72 horas. Utilizou-se a relação de uma pupa muscóide por fêmea parasitóide. A amostra de pupas frescas permitiu a emergência de 15 parasitóides/ pupa, em média, enquanto 10 parasitóides / pupa emergiram dos espécimens prévia e diretamente armazenados em freezer (-20ºC. Observou-se um acentuado decréscimo do desempenho reprodutivo dos microhimenópteros que exploraram os substratos previamente submetidos ao NL durante um, três e 15 minutos (: 6,1; 5,5 e 5,7, respectivamente. A dissecação das pupas hospedeiras revelou um expressivo número de pteromalídeos imaturos, nas amostras que foram expostas ao NL, e de adultos faratos, em todos os tratamentosThe reproductive performance of Nasonia vitripennis Walker (Hymenoptera: Pteromalidae was evaluated on pupae of Chrysomya megacephala Fabricius (Diptera: Calliphoridae kept at -20ºC, during 77 days, with and no previous passage for liquid nitrogen (NL by one, three and 15 minutes. Control groups were characterized for fresh pupae hosts. There was one pupa for each parasitoid. The sample of fresh pupae exhibited average of 15 emergent parasitoids / pupa while pupae stored directly at freezer (-20ºC presented an average of 10 emergent parasitoids / pupa. In the samples exposed at one, three and 15 minutes in NL, accentuated decrease was observed on emergent hymenopterans reproductive performance (: 6.1; 5.5 and 5.7 respectively. The dissection of pupae revealed a large number of immature pteromalid in the groups with liquid nitrogen passage and farate adults in

  20. Perfil eletroforético das proteínas séricas de serpentes Crotalus durissus terrificus (cascavel criadas em cativeiro Serum protein electrophoresis profile of the rattlesnake Crotalus durissus terrificus kept in captivity

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    Joandes Henrique Fonteque

    2009-06-01

    Full Text Available As serpentes peçonhentas dos gêneros Bothrops e Crotalus têm sido mantidas em cativeiro visando à extração de venenos para a produção de imunobiológicos. O conhecimento da fisiologia desses animais e as alterações na concentração de proteínas e suas frações séricas são importantes para a identificação precoce de importantes enfermidades que cursam com estados de hipoproteinemia e hiperproteinemia. O objetivo do trabalho foi determinar a concentração de proteína total e o perfil eletroforético das proteínas séricas de serpentes Crotalus durissus terrificus (cascavel criadas em cativeiro. Foram colhidas amostras de sangue da veia coccígea ventral de 21 serpentes adultas e sadias, divididas em dois grupos: Grupo 1 de 12 machos com peso médio de 588,89±193,55g, e Grupo 2 de nove fêmeas com peso médio de 708,33±194,04g. A proteína total sérica foi determinada pelo método de refratometria e a eletroforese em gel de agarose. Obtiveram-se valores da proteína total sérica (g/dL de 4,51±0,50 para machos e de 4,82±0,72 para fêmeas, e para machos e fêmeas de 4,64±0,61. Foram identificadas pela eletroforese quatro frações protéicas (g/dL: albumina, a, b, g-globulinas e calculada a relação albumina:globulina. As serpentes fêmeas apresentaram maiores valores para as variáveis, albumina e para a relação albumina/globulina (AG diferindo significativamente (PThe poisonous snakes of the genera Crotalus and Bothrops have been kept in captivity with the purpose of extracting poison for the production of immunobiological. Knowledge of the physiology of these animals and serum proteins concentration changes are important for early identification of major diseases which lead to states of hypoproteinemia and hyperproteinemia. The objective was to determine the concentration of total protein and serum protein electrophoresis profile of Crotalus durissus terrificus (rattlesnake in captivity. Blood samples were taken from

  1. Níveis de cloreto de sódio para aves de corte da linhagem Colonial criadas em semiconfinamento Sodium chloride levels for Colonial broiler chickens reared in free-range system

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    Sandra Regina Freitas Pinheiro

    2011-07-01

    Full Text Available Objetivou-se determinar níveis de inclusão de cloreto de sódio (NaCl em rações para aves machos e fêmeas da linhagem Colonial nas fases inicial (1 a 28 dias, de crescimento (28 a 56 dias e final (56 a 84 dias criadas em semiconfinamento. Em cada ensaio, 480 aves com idade correspondente à fase de criação foram alojadas em 24 unidades experimentais contendo áreas de abrigo e de pastejo. O delineamento experimental utilizado foi o inteiramente casualizado, em esquema fatorial 4 × 2 (níveis de NaCl e sexos, totalizando oito tratamentos e três repetições de 20 aves. Os níveis de NaCl avaliados foram: 0,20; 0,40; 0,60 e 0,80% na fase inicial; 0,10; 0,30; 0,50 e 0,70% na fase de crescimento e 0,10; 0,25; 0,40 e 0,55% na fase final. Foram avaliados o ganho de peso, o consumo de ração, a ingestão de água, a conversão alimentar, o potencial hidrogeniônico, a pressão parcial de gás carbônico no sangue, a concentração de hematócritos e os teores de sódio e cloro no sangue. Para a fase inicial, o nível recomendado é de 0,53% de NaCl na ração para aves de ambos os sexos. Para a fase de crescimento, os níveis de 0,40 e 0,43% de NaCl, respectivamente, para machos e fêmeas e, para a fase final, de 0,25% para aves de ambos os sexos, atendem às exigências nutricionais.The objective of this work was to determine inclusion levels of sodium chloride (NaCl in diets for male and female Colonial strain birds in the starter (from one to 28 days of age, growing (from 28 to 56 days of age and finisher (from 56 to 84 days of age phases reared in free-range system. In each trial, 480 birds at age corresponding to the rearing phase were housed in 24 experimental units containing areas of shelter and pasture. The experimental design used was a completely randomized in a 4 × 2 factorial arrangement (NaCl levels × sex, totaling eight treatments and three replications of 20 birds. Levels of NaCl evaluated were: 0.20, 0.40, 0.60 and 0.80% in

  2. Exigências de lisina digestível para aves de corte da linhagem ISA Label criadas em semiconfinamento Digestible lysine requirements for ISA Label broilers reared in free-range system

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    D.C. Nagib Nascimento

    2009-10-01

    Full Text Available Foram realizados três experimentos para determinar as exigências de lisina digestível para aves da linhagem ISA Label, de ambos os sexos, criadas em semiconfinamento durante as fases: inicial (1 a 28 dias, de crescimento (28 a 56 dias e final (56 a 84 dias. Em cada experimento, foram utilizadas 480 aves, alojadas em 24 piquetes, cada um contendo abrigo coberto de 3,13m² e área de pastejo de 72,87m². O delineamento experimental utilizado foi o inteiramente ao acaso, em esquema fatorial 4x2 (níveis de lisina e sexo com três repetições de 20 aves cada. Os níveis de lisina digestível avaliados foram: 0,850; 0,970; 1,090 e 1,210% na fase inicial; 0,750; 0,870; 0,990 e 1,110% na fase de crescimento e 0,640; 0,760; 0,880 e 1,000% na fase final. Foram mensuradas as variáveis de desempenho, característica de carcaça, deposição de proteína e gordura corporal, peso e teor de proteína das penas. Com base nos resultados de desempenho, recomendam-se 1, 041; 1,006 e 0,760% de lisina digestível em rações para aves ISA Label nas fases inicial, de crescimento e final, respectivamente.Three assays were carried out to determine digestible lysine requirement for ISA Label, both genders, in free-range system on starter (1 to 28 days, growing (28 to 56 days e finishing phases (56 to 84 days. A total of 480 birds were distributed into 24 pens, each one composed by shelter (3.13m² and pasture (72.87m². The experimental design was a completely randomized in factorial arrangement (four levels of lysine and two genders with three replicates of 20 birds. The digestible lysine levels were 0.85, 0.97, 1.09, and 1.21% for starter; 0.75, 0.87, 0.99, and 1.11% for growing; and 0.64, 0.76, 0.88, and 1.00% for finishing phases. The analyzed parameters were performance, carcass yield, protein and fat deposition, weight, and protein in the feathers. Based on bird performance the estimated digestible lysine level, was 1.041, 1.006, and 0.760% for the starter

  3. Developmental rates of immatures of three Chrysomya species (Diptera: Calliphoridae) under the effect of methylphenidate hydrochloride, phenobarbital, and methylphenidate hydrochloride associated with phenobarbital.

    Science.gov (United States)

    Rezende, Fábio; Alonso, Marcela A; Souza, Carina M; Thyssen, Patrícia J; Linhares, Arício X

    2014-05-01

    Entomotoxicology is focused on obtaining data on necrophagous entomofauna, for criminal investigations purposes. This study aimed to evaluate the effect of different concentrations of methylphenidate hydrochloride, phenobarbital, and their association on the developmental rate, larval and pupal survivorship, and the interval of emergence of adults of Chrysomya albiceps (Wiedemann), Chrysomya megacephala (Fabricius), and Chrysomya putoria (Wiedemann) (Diptera: Calliphoridae). Considering the therapeutic dose (TD) of methylphenidate hydrochloride (0.29 mg/Kg), the concentrations tested were 10× TD, 50× TD, and 100× TD. For phenobarbital, the concentrations used were 1× TD (=150 mg/Kg), 3.3× TD, and 6.7× TD. For the association of the drugs, the combinations used were 10× TD-methylphenidate hydrochloride plus 1× TD-phenobarbital, 50× TD-methylphenidate hydrochloride plus 3.3× TD-phenobarbital, and 100× TD-methylphenidate hydrochloride plus 6.7× TD-phenobarbital. The control group, without addition of drug, was maintained under the same conditions of temperature (25 ± 1 °C), humidity (70 ± 10%), and photoperiod (12 h). Specimens of each group were weighed every 12 h until pupariation. The developmental rate of the three Chrysomya species immatures was monitored. For C. albiceps the developmental time was delayed in 24 h for methylphenidate hydrochloride group and in 12 h for the phenobarbital and the drugs association groups. The effect was observed only at specific ages for C. megacephala, without altering the developmental time. For C. putoria, the developmental time was delayed in 12 h for methylphenidate hydrochloride group and in 24 h for the phenobarbital and the drugs association groups. The emergence interval was similar among all experimental groups, but larval and pupal viabilities were affected in different ways.

  4. Isolation of the Male-Specific Transformer Exon as a Method for Immature Specimen Sex Identification in Chrysomya megacephala (Diptera: Calliphoridae).

    Science.gov (United States)

    Smith, J L; Wells, J D

    2017-03-01

    Being able to efficiently differentiate between male and female individuals in the immature forms of insects allows for investigations into sexually dimorphic patterns of growth rates and gene expression. For species lacking sex-specific morphological characteristics during these periods, alternative methods must be devised. Commonly, isolation of sex determination genes reveals sex-specific band patterns and allows for markers that can be used in insect control. For blow flies, a family that includes flies of medical and forensic importance, sex has previously been identified in some members using the male-specific exon in the transformer gene. This gene is relatively conserved between members of the genera Cochliomyia and Lucilia (Diptera: Calliphoridae), and we isolated a portion of this gene in an additional forensically and medically important blow fly genus using the widespread Chrysomya megacephala (F.). We found a relatively high level of conservation between exons 1 and 2 of transformer and were able to amplify a region containing the male-specific exon in C. megacephala. A sex-specific molecular diagnostic test based on the presence of sexually dimorphic PCR product bands showed the expected genotype for adults and intrapuparial period specimens of known sex. The same result could be obtained from single third-instar larval specimens, opening up the possibility to not only determine if development rates are sex dependent, but also to investigate the development of sexually dimorphic traits of interest in C. megacephala. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  5. Níveis nutricionais de fósforo disponível para aves de corte ISA Label criadas em semiconfinamento Nutritional levels of available phosphorus for ISA Label broilers chickens reared in free-range system

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    Sandra Regina Freitas Pinheiro

    2011-02-01

    Full Text Available Foram realizados três ensaios para determinar os níveis nutricionais de fósforo disponível (Pd para machos e fêmeas da linhagem ISA Label nas fases inicial (1 a 28 dias, crescimento (28 a 56 dias e final (56 a 84 dias criadas em semiconfinamento. Em cada ensaio, 480 aves com idade correspondente à fase de criação foram alojadas em 24 unidades experimentais contendo áreas de abrigo e de pastejo. O delineamento experimental utilizado foi o inteiramente casualizado, em esquema fatorial 4 × 2 (níveis de Pd e sexos com três repetições de 20 aves. Os níveis de fósforo disponível avaliados foram: 0,25; 0,36; 0,47 e 0,58% na fase inicial; 0,18; 0,31; 0,44 e 0,57% na fase de crescimento; e 0,14; 0,27; 0,40 e 0,53% na fase final. Foram avaliados o ganho de peso, consumo de ração, consumo de Pd, conversão alimentar, teores de fósforo, cálcio e cinzas na tíbia e resistência à quebra óssea. De acordo com os resultados, o nível ótimo de Pd na ração na fase inicial, para machos e fêmeas são de 0,39 e 0,49%, que correspondem ao consumo de 3,94 e 3,96 g de Pd/ave, respectivamente. Para a fase de crescimento, recomenda-se 0,35% de Pd na ração para aves de ambos os sexos, que correspondem a consumo de 8,45 e 6,70 g de Pd/ave. Na fase final, recomendam-se os níveis de 0,32 e 0,30% de Pd, que correspondem a consumos de 12 e 9,5 g de Pd/ave para machos e fêmeas, respectivamente.It was performed three trials to determine the nutritional levels of available phosphorus (aP for males and females of the strain ISA Label in the starter (1 to 28 days, growing (28 to 56 days and finisher (56 to 84 days phases, reared in a free-range system. In each trial, 480 birds with age corresponding to rearing phase were housed in 24 experimental units with shelter and pasture areas. It was used a complete random experimental design, in a 4 × 2 factorial scheme (levels of aP and sexes with three replicates of 20 birds. The levels of aP evaluated were

  6. Variability of the morphometric features of Calliphora vicina (Diptera, Calliphoridae under the varying and constant micro-climatic condi-tions

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    L. I. Faly

    2013-03-01

    Full Text Available Variability of the main morphometric features of imago flies Calliphora vicina R.-D. (Diptera, Calliphoridae of two samples was studied. First sample consists of individuals caught in the wild (park ecosystems of Dnipropetrovsk, the second one – specimens cultured in the laboratory under the constant temperature and humidity. Possible using of C. vicina R.-D. as a bioindicator of anthropogenic factors is analysed. Environmental factors may act as the stimulators of adaptive changes in physiological functions, as the constraints that cause impossibility of the existence of certain species in particular conditions, and as modifiers that determine the morpho- anatomical and physiological changes in organisms. The most significant differences between studied samples were found for the width (“laboratory” individuals are characterized by larger head size and for the length of limbs segments. The fluctuating range of the head width in specimens collected in the wild is much wider, due to the heterogeneity of the micro-climatic conditions of the larvae development and trophic resources. Maximal negative asymmetry of the head width is observed in individuals C. vicina R.-D. of the “natural” sample as compared with “laboratory” individuals. Among imagoes caught in the wild the individuals with a relatively wide head are dominated, as evidenced by the large positive value of kurtosis. At the same time, the distribution of values in “laboratory” individuals is almost normal. In adults bred in the laboratory the shortening of segments of the leg pair I is observed in comparison with the individuals of “natural” sample. Similar results were recorded for other insect groups cultivated in a laboratory. For most other linear measurements of the C. vicina R.-D. body the differences between samples are not registered. Ephemeral existence of the substrate of blow flies leads to higher prevailing evolutionary adaptation of species to varying

  7. 中国丽蝇科三新种(双翅目:丽蝇科)%Three new species of calliphoridae from Sichuan, China (Dpitera)

    Institute of Scientific and Technical Information of China (English)

    冯炎; 薛万琦

    2000-01-01

    1982-1987年采自中国四川山区蝇类标本中,发现丽蝇科Calliphoridae丽蝇族Calliphorini三新种:宽颜拟粉蝇Polleniopsis latifacialis sp.nov.,红头陪丽蝇Bellardia ruffceps sp.nov.,华夏蚓蝇Onesia huaxiaae sp.nov.模式标本存沈阳师范学院昆虫研究所.%This paper describes three new species of Calliphoridae from mountinous region of Sichuan Prov., named Polleniopsis latifacialis sp. nov., Bellardia ruficeps sp. nov. and Onesia huaxiaae sp. nov., The type specimens are kept in the lnstitute of Entomology, Shenyang Normal University. 1. Polleniopsis latifacialis sp. nov. (Figs, 1 - 5 ) ♂ :Body length 9.0 - 10.0mm. This new species similar to the Polleniopsis viridiventris Chen et Fan, 1988 from Xizang Auton. Reg.. but differs from the al bristles present; basicosta yellow; fore tibia with a row of ad setae(6 -7); ac (1-2) + 3; acrophallus of aedeagus curvous forward. ♂ :Body length 8.0mm. Frons about one-third the head width; interfrontalia red brown, both sides parallel; frontal triangle not reaching the anterior margin of frons; inferior orbitals 10, superior orbitals 2 inclined forward; width of parafacials about 2.3 times the width of 3rd antennal segment; abdomen with median stripein trace; 3rd tergite without postmarginal bristle. Type locality : Holotype.♂, Mt. Erlang(tree farm, 2680m; 29°53′N, 102°10′E), Sichuan Prov., 1982 - 08 - 26, collected by Feng Yah; paratype 6 ♂ ♂, 1♂, 1988 - 07 - 12, collected by Yang Shibin. Locality same as holotype. 2 Bellardia ruficeps Sp. nov. (Figs. 6 - 10) ♂ :Body length 7.0mm. The new species differs from all other known species of this genus in having the combination of the following characters: Front surface of head yellowbrown in largely; facial carina well developed; outer posthumeral bristles present; without anterioria; r1 vein on dorsum of apical half with a row of setae(6- 7); surstyli of male broader and larger, reflex platelet on hypophallus

  8. Tratamiento de las úlceras crónicas en los miembros inferiores con un equivalente cutáneo autólogo y desbridación con larvas de Lucilia sp. (Diptera: Calliphoridae. Reporte de un caso. Chronic lower limb ulcers treatment with autologous skin equivalent and larvae debriding (diptera calliphoridae

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    Sergio Estrada Mira

    2007-12-01

    Full Text Available Introducción: las úlceras en los miembros inferiores constituyen una causa importante de hospitalización y deterioro en la calidad de vida de los pacientes, al interferir con sus actividades laborales y sociales. Estas lesiones obedecen a diferentes enfermedades, la más frecuente de las cuales en Medellín es la insuficiencia venosa; una vez diagnosticadas, existen muchas alternativas de tratamiento, que buscan crear un lecho apropiado para el cierre de la herida. El uso de larvas para el desbridamiento es una alternativa rápida y de bajo costo, con la cual se logra un tejido de granulación adecuado para la aplicación de equivalentes cutáneos autólogos, que constituyen una opción para pacientes refractarios a otros tratamientos. Materiales y métodos: se seleccionó y evaluó a una paciente de acuerdo con los criterios de inclusión establecidos para el estudio. Para desbridar la úlcera se recurrió a la aplicación de larvas de Lucilia sp. (Diptera: Calliphoridae, obtenidas a partir de huevos previamente desinfectados con hipoclorito de sodio y sembrados en un medio de cultivo con antibióticos. Se aplicaron las larvas directamente en la lesión y posteriormente se procedió a tapar esta con muselina, permitiendo la obtención de oxígeno y facilitando el drenaje del tejido necrótico. Las larvas se dejaron por 48 horas, al cabo de las cuales se retiraron con una pinza estéril y se descartaron en alcohol al 70%; luego se procedió a evaluar la limpieza de la lesión. Este procedimiento se llevó a cabo en el Laboratorio de Entomología (GIEM de la Universidad Antioquia. Se procesó una biopsia de piel de la paciente, obtenida siguiendo un protocolo previamente establecido, para obtener queratinocitos y fibroblastos. Para el cultivo primario de queratinocitos el 90% de las células obtenidas de la biopsia se sembró en cajas de cultivo de 75 cm2, en presencia de 7 x 106 células de la línea 3T3- Swiss tratadas con mitomicina C (10 g

  9. Aspectos biológicos de Chrysoperla externa (Hagen, 1861 (Neuroptera: Chrysopidae alimentada com Bemisia tabaci (Gennadius, 1889 biótipo B (Hemiptera: Aleyrodidae criada em três hospedeiros Biological aspects of Chrysoperla externa (Hagen, 1861 (Neuroptera: Chrysopidae fed on Bemisia tabaci (Gennadius, 1889 biotype B (Hemiptera: Aleyrodidae reared in three hosts

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    Cláudio Gonçalves Silva

    2004-04-01

    Full Text Available Estudaram-se alguns aspectos biológicos de adultos de Chrysoperla externa (Hagen oriundos de larvas alimentadas com ninfas da mosca-branca Bemisia tabaci (Gennadius biótipo B, criadas em folhas de pepino (Cucumis sativus var. caipira, couve (Brassica oleraceae var. acephala e na planta invasora leiteiro (Euphorbia heterophylla. Discos foliares dos hospedeiros contendo ninfas de terceiro e quarto estádios da mosca-branca foram acondicionados em placas de Petri contendo ágar-água a 1%. Em cada placa foi colocada uma larva de C. externa recém-eclodida, onde permaneceram até a emergência dos adultos. Foram avaliados o peso logo após a emergência, os períodos de pré-oviposição, oviposição, efetivo de oviposição, pós-oviposição, número total de ovos produzidos por fêmea e longevidade. Avaliaram-se, também, o período embrionário e a viabilidade dos ovos, coletando-se, uma vez por semana, uma amostra de 10% do total de ovos produzidos no dia, ao longo de todo o período reprodutivo. O peso de machos e fêmeas não diferiu significativamente em função do hospedeiro da presa, sendo em média de 4,7 mg. De maneira geral, adultos de C. externa oriundos de larvas alimentadas com ninfas de B. tabaci biótipo B criadas em leiteiro apresentaram performance inferior em relação àqueles oriundos do pepino e da couve. O período de oviposição foi, em média, de 49,5 dias para o pepino e a couve, e de 31,6 dias para o leiteiro. A produção de ovos foi reduzida em aproximadamente 50% em leiteiro, sendo de 293,8 ovos; no pepino e couve, a média foi de 591,3 ovos. Ninfas de B. tabaci biótipo B criadas em leiteiro causaram uma redução da viabilidade dos ovos de C. externa, que foi 62,8% e 57,7% inferior ao verificado para o pepino e a couve, respectivamente.The aim was to study some biological aspects of adults of Chrysoperla externa (Hagen, from larvae fed on nymphs of third and fourth stages of Bemisia tabaci (Gennadius biotype B

  10. Biologia e exigências térmicas de cinco linhagens de Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae criadas em ovos de Tuta absoluta (Meyrick (Lepidoptera: Gelechiidae Biology and thermal requeriments of five Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae strains, reared on eggs of Tuta absoluta (Meyrick (Lepidoptera: Gelechiidae

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    Dirceu Pratissoli

    2006-12-01

    Full Text Available O objetivo desta pesquisa foi estudar a biologia e as exigências térmicas de cinco linhagens de Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae coletadas em áreas comerciais de tomate, no Estado do Espírito Santo, em ovos da traça-do-tomateiro Tuta absoluta (Meyrick (Lepidoptera: Gelechiidae, sob diferentes temperaturas constantes e flutuantes. A duração do ciclo de desenvolvimento (ovo-adulto das cinco linhagens de T. pretiosum criadas em ovos de T. absoluta foi afetada significativamente pela temperatura, na faixa das constantes (15 a 30°C. Esse mesmo fato não foi observado nas temperaturas alternadas (26/14 e 29/16°C. O desempenho das linhagens nas diferentes temperaturas mostrou que L2 e L5 apresentaram os melhores resultados quanto à taxa de emergência de seus descendentes. A razão sexual das cinco linhagens foi afetada pela temperatura, havendo diferença significativa entre os valores obtidos nas faixas das temperaturas (constante e alternada estudadas. As linhagens de T. pretiosum apresentaram uma temperatura base (tb que variou de 8,70 a 13,45°C, e uma constante térmica (K de 90,09 a 159,43 graus/dia.The objective of this research was to evaluate the biology and the thermal requeriments of five strains of Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae collected in commercial areas of tomato, in Espírito Santo State, on eggs the tomato leafminer Tuta absoluta (Meyrick (Lepidoptera: Gelechiidae under constant and alternating temperatures. The duration of the development cycle (egg-adult of the five strains of T. pretiosum reared in eggs of T. absoluta was affected significantly by the temperature, in the band of the constants (15 to 30°C. This same fact was not observed in the alternated temperatures (26/14 and 29/16°C. The performance of the strains in the different temperatures, showed that L2 and L5 presented the best results concerning the emergency rate of their descendants of viability

  11. Perfil hematológico de búfalas da raça Murrah, criadas ao sol e à sombra, em clima tropical da Amazônia Oriental Hematologic profile of murrah buffalo cows, raised in the sun and under shade, at tropical climate of the eastern amazon

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    Jamile Andréa Rodrigues Silva

    2011-01-01

    Full Text Available Foram avaliadas as respostas hematológicas de 20 búfalas, criadas ao sol (grupo - SS e à sombra (grupo - CS, em Belém, Pará. Os animais do grupo CS (n = 10 estavam em sistema silvipastoril, com Racosperma mangium e os do SS (n = 10, em piquetes sem sombra, em pastagem de Brachiaria humidicola, água para beber e sal mineral. Foram mensuradas temperatura do ar (TA, umidade relativa do ar (UR e temperatura de globo negro, em cada tratamento. A coleta de sangue para eritrograma e número total de leucócitos foi realizada a cada 14 dias, às 13 h, durante o ano de 2009. Através da análise de variância constatou-se que em todos os períodos do ano, a TA e índice de temperatura de globo e umidade (ITGU foram diferentes (P We evaluated the hematologic responses of 20 female buffaloes raised under full sun (group NS and shade (group SG at Belem, Para , Brazil. The animals in the SG group (n = 10 were maintained in paddocks under a silvopastoral system with Racosperma mangium, and the ones in the NS (n = 10, in paddocks with Brachiaria humidicola, with drinking water and mineral salt, but without access to shade. Air temperature (AT, relative air humidity (RH and black global temperature (BGT were measured for each treatment. Blood sampling for erythrogram and leukocyte count were undertaken at intervals of 14 days, and carried out at 1:00 p.m., during 2009. AT and black global temperature and humidity index (BGHI were different (P < 0.05 throughout the year, and their highest values were observed in the NS group. During the rainier period, the SG group presented the highest values of leukocytes (P < 0.05, although in the transition and low rainy periods, the highest values were observed in the NS group. In the low rainy period, the erythrocytes count showed the highest values in the NS group. The highest levels of hemoglobin (P < 0.05 were observed during the transition and low rainy periods. Only hemoglobin presented a significant and

  12. Efeito do processamento do milho sobre o desempenho e composição de carcaça de piaba (Leporinus friderici criada em tanques-rede Effect of the corn processing in the performance and carcass composition of the piaba (Leporinus friderici in the cage farm

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    Jodnes Sobreira Vieira

    2005-04-01

    Full Text Available Com este experimento objetivou-se verificar os efeitos dos processamentos do milho sobre o desempenho e composição de carcaça de piaba (Leporinus friderici criada em tanques-rede. Foi conduzido na Usina Hidrelétrica da Companhia Energética de Minas Gerais (CEMIG, município de Itutinga, com duração de 45 dias. Foram utilizados 24 tanques-rede de 1 M³, com 480 alevinos. O delineamento foi em blocos casualizados, com 4 tratamentos (rações formuladas com milho moído, extrusado, laminado e peletizado e 3 repetições. Os dados foram submetidos à análise de variância e as médias dos tratamentos, comparadas pelo teste Scott & Knott. Os resultados não mostraram diferença significativa para os parâmetros de desempenho (peso final médio, consumo aparente de ração médio, conversão alimentar aparente, ganho de peso total médio, ganho de peso diário médio (P>0,05, porém, o milho extrusado e o moído aumentaram o teor de proteína (PThe experiment with the objective of verify the effects of the processing (extrusion, rolling and pelleting of corn on the performance and composition of the Piaba (Leporinus frederici carcass, was conducted in the Usina Hidreletrica da Companhia Energética de Minas Gerais - CEMIG (Minas Gerais Hydroelectric Station, in the Itutinga- MG , with the lenght of 45 days. The experiment was undertaken in 24 cages of 1M³, 480 fingerlings were utilized, they were distributed into blocks. The four experimental rations were formulated based in corn meal, extruded corn, rolled corn and pelleted corn. The data obtained were submitted to the analysis of variance and the comparison of means of the treatments by the Scott & Knott test. The results showed any statistical difference in the performance parameters. However, the use of extruded corn and corn meal increased protein content (P<0.05 and decreased fat content (P<0.05 of the carcass traits, these results showed, improved protein utilization for structural

  13. Níveis iônicos e enzimáticos de cutias (Dasyprocta sp. hígidas, criadas em cativeiro, influência do sexo e da idade Ion and enzymatic levels of healthy agouti (Dasyprocta sp. raised in captivit. Influence of gender and age

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    E.E.A. Ribeiro

    2008-06-01

    Full Text Available Determinou-se o perfil do ionograma e enzimas hepáticas de cutias (Dasyprocta sp. saudáveis, criadas em cativeiro, como também se avaliou a influência de sexo, idade e interação sexo-idade. Foi adotado um delineamento inteiramente ao acaso, em arranjo fatorial 2 x 4 (dois sexos e quatro faixas etárias, com três repetições, totalizando 24 cutias. Foram determinados os valores para o cálcio (Ca, fósforo (P, cloretos (Cl, aspartato aminotransferase (AST, alanina aminotransferase (ALT, fosfatase alcalina (FA e gama-glutamiltransferase (GGT. Os valores médios obtidos foram: Ca= 7,62+2,59mg/dl; P= 3,91+1,41mg/dl; Cl= 58,63+16,45mg/dl; AST= 119,54+79,35UI/ml; ALT= 28,08+15,53UI/ml; FA= 26,95+14,01UI/ml e GGT= 25,34+19,44UI/ml. O valor de P foi maior nas fêmeas e da FA nos machos. Os níveis de FA diminuíram com o aumento da idade.This research studied the profile of the ionogram and hepatic enzymes of healthy agoutis (Dasyprocta sp. raised in captivity as well as evaluated the influence of gender, age and interaction gender-age. It was used a completely randomized design, in a factorial arrangement of 2 x 4 (two genders and four age groups, with three repetitions, totaling 24 agoutis. The values were determined for calcium (Ca, phosphorus (P, chlorides (Cl, aspartate aminotransaminase (AST, alanine aminotransaminase (ALT, alkaline phosphatase (ALP, and gamma-glutamyltransferase (GGT. The mean values were: Ca= 7.62±2.59mg/dl; P= 3.91±1.41mg/dl; Cl= 58.63±16,45mg/dl; AST= 119.54±79.35UI/ml; ALT= 28.08±15.53UI/ml; ALP= 26.95±14.01UI/ml, and GGT= 25.34±19.44UI/ml. The value of P was larger in females and ALP in males. As the age increased, levels of ALP decreased.

  14. Comparison of salivary cortisol concentrations in Jaguars kept in captivity with differences in exposure to the public Comparação das concentrações de cortisol salivar em onças-pintadas criadas em cativeiro com diferenças de exposição ao público

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    Julio César Montanha

    2009-09-01

    Full Text Available For the most part, jaguars kept in captivity are used for educational and recreational purposes and it hasn't been determined the different impacts that public exposure would have on these animals. In this study, we compared the salivary cortisol, one of the stress indicator hormones, in seven captive jaguars exposed to the high and low public visitation. Saliva was collected using an absorbent material that was chewed by the animals and subsequently analyzed through an immunoenzymatic assay. Salivary cortisol concentrations were significantly higher in the jaguars with higher public exposure, which suggest a more hormonal stress response. These results indicated that jaguars, animals usually of solitary and secretive behavior, may have their welfare considerably diminished by increased public exposure. Additionally, design of the enclosures, without refuges and proximity of people, could predispose jaguars to psychological stress.Em geral, onças-pintadas criadas em cativeiro são usadas para fins educacionais e de recreação. No entanto, o impacto da exposição ao público sobre esses animais não tem sido avaliado. Neste estudo, foi comparado o impacto de dois diferentes níveis de exposição ao público em sete onças-pintadas, alojadas em dois criadouros, por meio da avaliação de um dos hormônios indicadores de estresse, o cortisol salivar. A saliva foi coletada usando um material absorvente que foi mastigado pelos animais e subsequentemente analisado por imunoenzimoensaio. As concentrações de cortisol salivar foram significativamente maiores em onças-pintadas com maior exposição ao público. Esses resultados sugerem que onças-pintadas, normalmente solitárias e esquivas na natureza, podem ter seu bem-estar consideravelmente diminuído pelo aumento de exposição ao público. Este estudo é o primeiro relato de mensuração de cortisol salivar em onças-pintadas.

  15. Redução da proteína da dieta com base no conceito de proteína ideal para tilápias-do-nilo (Oreochromis niloticus criadas em tanques-rede Dietary protein reduction based on ideal protein concept for Nile Tilapia (Oreochromis niloticus cultured in net pens

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    Daniele Botaro

    2007-06-01

    Full Text Available Avaliou-se o desempenho de tilápias-do-nilo (Oreochromis niloticus criadas em tanques-rede e alimentadas com dietas contendo 27,0 (controle; 25,2; 24,3 e 22,7% de proteína digestível. Aminoácidos cristalinos (L-lisina, DL-metionina e L-treonina foram adicionados à dieta considerando o conceito de proteína ideal e simulando o perfil de aminoácidos da dieta controle. Os peixes (34,63±19 g foram alimentados manualmente com dietas isoenergéticas (3.075 kcal de energia digestível/kg de dieta até saciedade aparente, três vezes ao dia, durante 91 dias. Utilizou-se um delineamento inteiramente casualizado, com quatro tratamentos, três repetições e 25 peixes/unidade experimental. Não foram observados efeitos dos níveis de proteína digestível sobre o ganho de peso, a conversão alimentar, a taxa de eficiência protéica, o peso da carcaça eviscerada, o rendimento de carcaça, o peso e o rendimento de filé, a sobrevivência e o hematócrito. Houve efeito quadrático dos níveis de proteína digestível sobre o consumo; o maior valor foi estimado para a dieta contendo 24,41% de proteína digestível e excreção de nitrogênio, na qual o melhor resultado estimado foi obtido com peixes que receberam a dieta contendo 24,92% de PD. Com a redução nos níveis de proteína digestível, observou-se aumento linear na retenção de nitrogênio. É possível reduzir o nível de proteína digestível, de 27 (29,1% de PB para 24,3% (26,6% de PB, em dietas para tilápias-do-nilo criadas em tanques-rede. Essa redução deve ser feita por meio da suplementação de aminoácidos (com base no conceito de proteína ideal, considerando o desempenho e o custo da dieta/kg ganho em filé.This study was carried out to evaluate the performance of Nile tilapia, cultured in net pens, and fed diets containing 27.0 (control, 25.2, 24.3, and 22.7% of digestible protein. Crystalline amino acids (L-lysine, DL-methionine and L-threonine were added to keep amino acid

  16. Influência do parasitismo por monogeas no desenvolvimento de tilápias-do-Nilo (Oreochromis niloticus (Linnaeus, 1757 criadas em sistemas de tanques-rede na represa de Capivara, PR The influence of branchial parasitism by monogenoid trematodes on the development of Nile tilapia(Oreochromis niloticus Linnaeus, 1757 bred in net-pond systems in Capivara Dam, PR

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    Rodrigo Zanolo

    2009-03-01

    Full Text Available Neste trabalho foi avaliada a influência do parasitismo branquial por monogenóideos no desenvolvimento de tilápias-do-nilo criadas em 4 tanques-rede, com volume de 4 m3 cada, durante aproximadamente 5 meses. Os peixes juvenis, apresentando peso médio inicial de 37,65 g provenientes de outras pisciculturas, foram estocados na densidade de 250 animais.m-3 e monitorados mensalmente até sua comercialização com peso médio final de 485,4 g. A prevalência desses ectoparasitas apresentou-se alta, entre 90 e 100% em todos os meses. Os maiores valores de intensidade média de infestação - IMI e abundância média de infestação - AM ocorreram durante os 2 primeiros meses de cativeiro, apresentando um novo aumento no último mês de criação. Os únicos parasitos da classe Monogenoidea presentes nas brânquias dos animais examinados foram da família Dactylogyridae. Os valores de oxigênio dissolvido, temperatura, pH, nitrito e amônia estiveram dentro da normalidade. Nessas condições, não houve diferenças significativas entre o fator de condição relativo - Kn médio entre os animais parasitados e não parasitados e também nos diferentes níveis de infestação. Assim mostrou que, dentro dessas condições de criação, a relação parasito-hospedeiro-ambiente apresentou- se em equilíbrio sem causar grandes prejuízos aos animais.Tilapias are fish originally from Africa which nowadays are commercially bred in almost 100 countries, being one of the most commercially bred species in the world. In this work the trematode population of the monogenoidea group present in the branchiae of Nile tilapias bred in 4 net-ponds with volume of 4 m3 each, was monitored during 5 months. The juvenile fish, presenting initial average weight of 37.65 g originated from other piscicultures, were stocked in the density of 250 animals.m-3 and monthly monitored until their commercialization, with final average weight of 485.4 g. The prevalence of these

  17. Parâmetros de crescimento e metabólicos da tilápia vermelha criada em tanques-rede flutuantes em açude de pequeno porte Growth and metabolic parameters of red tilapia reared in floating net cages in a small reservoir

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    Damares Perecim Roviero

    2000-05-01

    Full Text Available O objetivo deste estudo foi gerar informação sobre parâmetros de crescimento e metabólicos da tilápia vermelha criada em tanques-rede num açude de pequeno porte. Foram instalados 12 tanques-rede flutuantes (5m3, em um açude de 1 ha, estocados com 25, 50, 75 e 100 tilápias vermelhas revertidas por m3, e alimentadas com duas rações extrusadas comerciais (32 e 28% PB, durante 213 dias. Não foram observadas diferenças significativas (P > 0,05 entre as diferentes densidades de estocagem ao final do período experimental com relação aos parâmetros de crescimento: peso corporal, comprimento total, fator de condição, conversão alimentar aparente, taxa de sobrevivência, ganho de peso médio diário e taxa de crescimento específico. Também não foram observadas diferenças significativas (P > 0,05 no glicogênio hepático, índice hepato-somático e lipídio hepático entre as densidades testadas. Contudo, a glicemia apresentou uma pequena diminuição entre o início do experimento e o 64o dia, retornando ao nível inicial a partir do 118o dia. O nível de glicogênio hepático foi mais elevado (P o dia, e o lipídio hepático apresentou maior valor no final do período experimental (P The objective of this study was to provide information on growth and metabolic parameters of red tilapia reared in cages in a small reservoir. Twelve floating net cages (5 m3 placed in an 1-ha reservoir were stocked with 25, 50, 75 and 100 sex-reversed red tilapias/m3, fed with two commercial floating feeds (32 and 28% crude protein, during 213 days. No effect (P > 0.05 of stocking density was observed at the end of the experimental period on body weight, body length, condition factor, feed conversion rate, survival rate, mean daily weight gain and specific growth rate. No differences (P > 0.05 on liver glycogen, liver somatic index (LSI, and total liver lipid were verified among the tested densities. However, blood glucose decreased slightly (P > 0

  18. Biochemical polymorphisms and genetic relationships between Brazilian and foreign breeds of pigs reared in Brazil Polimorfismos bioquímicos e o relacionamento genético entre raças suínas brasileiras e estrangeiras criadas no Brasil

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    Cláudia Helena Tagliaro

    1999-06-01

    Full Text Available The genetic variability of 14 protein systems encoded by 15 structural loci was investigated in blood samples of Piau and Caruncho pig breeds. The results were compared with those obtained previously for samples of Landrace, Large White, Duroc and Mouro. The degree of genetic variability obtained for Piau (He=0.114 was similar to that estimated for other breeds reared in Brazil (Landrace, He=0.116; Large White, He=0.119; Duroc, 0.095; Mouro, He= 0.130. Caruncho showed the lowest variability (He= 0.056. The gene frequencies at the polymorphic loci were used to evaluate the usefulness of these systems for paternity testing and the combined probabilities of paternity exclusion were estimated at 58% for the Piau and 36% for the Caruncho breed. Analysis of genetic distances revealed that the greatest similarity observed was between Piau and Landrace (D=0.042. Caruncho showed the greatest divergence among all breeds compared and the distances between this breed and others range from 0.107 (with Landrace to 0.176 (with Duroc. The tree constructed by UPGMA and Rogers’ Distance gave a topology in which Piau and Mouro joined with the European breeds (Landrace and Large White whereas Caruncho was separated from all the other breeds. The results of the analysis of the Caruncho samples should be interpreted with caution since the number of animals studied was small.Foi investigada a variabilidade genética de 14 sistemas protéicos codificados por 15 locos estruturais em amostras de sangue de suínos das raças Piau e Caruncho. Os resultados foram comparados com àqueles obtidos previamente para amostras de Landrace, Large White, Duroc e Mouro. O grau de variabilidade genética obtida para Piau (He=0,114 foi similar àquelas estimadas para outras raças criadas no Brasil (Landrace, He=0,116; Large White, He=0,119; Duroc, 0,095; Mouro, He= 0,130. Caruncho apresentou a menor variabilidade (He= 0,056. A partir das freqüências gênicas dos locos polim

  19. Relações entre tamanho de sedimentos erodidos, velocidade da enxurrada, rugosidade superficial criada pelo preparo e tamanho de agregados em solo submetido a diferentes manejos Relationships between size of eroded sediments, runoff velocity, surface roughness created by tillage, and size of aggregates in a soil submmited to different managements

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    Leandro Bochi da Silva Volk

    2009-10-01

    Full Text Available Vários fatores determinam a seletividade do processo de erosão hídrica pluvial no que se refere ao tamanho dos sedimentos transportados na enxurrada. Dentre eles, destacam-se a intensidade da chuva e da enxurrada a ela associada, a textura e o grau de consolidação da camada superficial do solo, a forma em que a erosão ocorre (entre sulcos, sulco ou voçoroca, a cobertura do solo por resíduos culturais, o microrrelevo do terreno ou a rugosidade superficial resultante do seu preparo e o tamanho e a estabilidade dos agregados do solo. Considerando isso, realizou-se este trabalho com o propósito de estabelecer relações quantitativas entre o índice D50 da distribuição de tamanho dos sedimentos erodidos, a velocidade da enxurrada, o índice IR da rugosidade superficial do solo criada pelo preparo e o diâmetro médio ponderado (DMP dos agregados do solo, em solo submetido a diferentes formas de manejo. O estudo foi desenvolvido em campo, na Estação Experimental Agronômica da Universidade Federal do Rio Grande do Sul (EEA/UFRGS, em Eldorado do Sul (RS, aplicando-se chuva simulada sobre um Argissolo Vermelho com textura francoargiloarenosa na camada superficial e declividade média de 0,115 m m-1. Esse solo havia sido submetido ao uso agrícola de diferentes modos (cultivos contínuo e interrompido, com diferentes sequências culturais (gramíneas e leguminosas de inverno e verão, implantadas em fileira, usando a técnica de semeadura direta, durante 7,5 anos (partindo da condição original de campo nativo. Efetuaram-se sete testes de erosão na pesquisa, cada um deles na intensidade constante de chuva de 63,5 mm h-1 e com duração de 1,5 h, usando o simulador de chuva de braços rotativos e parcelas experimentais com dimensões de 3,5 x 11,0 m cada uma. Os referidos testes de erosão foram realizados nas seguintes condições físicas de superfície do solo: (a solo não-mobilizado, com completa e nenhuma cobertura por res

  20. Exigências de proteína bruta e energia metabolizável para codornas japonesas machos criadas para a produção de carne Energy and protein requirements for male Japanese quails reared for meat production

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    N.T.E. Oliveira

    2002-04-01

    Full Text Available O experimento objetivou estimar as exigências de proteína bruta (PB e energia metabolizável (EM para máximo desempenho de machos de codornas japonesas criadas para a produção de carne e determinação da idade de abate que resultasse em peso máximo das aves. Quatrocentos e cinqüenta codornas foram utilizadas em delineamento experimental de blocos ao acaso, com cinco repetições de seis codornas por unidade experimental. Os tratamentos, de dietas formuladas a partir da combinação de cinco níveis de PB (18, 20, 22, 24 e 26% e três níveis de EM (2800, 3000 e 3200kcal/kg de ração, foram alocados nas parcelas e os quatro períodos experimentais nas sub-parcelas. As variáveis estudadas durante os quatro períodos (5 a 16; 16 a 27; 27 a 38 e 38 a 49 dias de idade foram consumo de ração (CR, ganho de peso (GP, conversão alimentar (CA e peso vivo (PV. No período total (5 a 49 dias de idade foram estudados consumo de ração acumulado (CRA, ganho de peso acumulado (GPA e conversão alimentar acumulada (CAA. As exigências estimadas de PB e EM durante o primeiro (5 a 16 e terceiro (27 a 38 períodos foram 26 e 2800 e 18% e 3200kcal/kg de ração, respectivamente. A exigência de PB no segundo período (16 a 27 dias de idade foi de 26%, não sendo possível estimar a exigência energética. No quarto (38 a 49 dias de idade período experimental a exigência estimada de EM foi de 3200kcal/kg de ração, não sendo possível estimar a exigência protéica. No período total as exigências protéica e energética, estimadas para máximos ganhos de peso acumulados, foram de 26% e 3200kcal/kg de ração, respectivamente. As idades estimadas que resultaram em PV máximo dependeram do nível de PB e de EM da dieta, variando de 57 a 85 e 60 a 74 dias, respectivamente.The experiment aimed to estimate the crude protein (CP and metabolizable energy (ME requirements for maximum performance of male Japanese quails reared for meat production purpose

  1. Associação da medroxiprogesterona ao protocolo Ovsynch para inseminação artificial em tempo fixo de búfalas cíclicas (Bubalus bubalis criadas na Amazônia Oriental The association of medroxyprogesterone to the Ovsynch protocol for the fixed time artificial insemination of cyclic buffaloes (Bubalus bubalis in the Eastern Amazon

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    Alexandre Rossetto Garcia

    2008-01-01

    Full Text Available O presente estudo visou avaliar os efeitos da associação da medroxiprogesterona (análogo sintético da progesterona ao protocolo Ovsynch sobre o crescimento folicular, a ovulação e a taxa de concepção de búfalas criadas na Amazônia Oriental (Tracuateua-PA. Vinte e sete fêmeas adultas (G1 n=14 e G2 n=13, cíclicas, sem bezerro ao pé e com ECC 3,5 foram submetidas a Ovsynch. Os animais do G2 receberam 60 mg de medroxiprogesterona entre D0 e D7 (D0=início do tratamento. A ultra-sonografia ovariana foi realizada nos D 0, 7, 9 e 10. O contingente de folículos pequenos diferiu no D7 (G1: 4,57±0,60 versus G2: 6,54±0,67; P=0,05. Tempo e tratamento influenciaram o diâmetro folicular no D7. O crescimento do folículo dominante entre D7 e D9 foi maior nos animais tratados (G1: 2,05±0,49 mm/dia versus 3,48±0,41 mm/dia; P0,05. Os achados sugerem que a medroxiprogesterona (1 aumenta recrutamento folicular e retarda o crescimento dos folículos com diâmetro maior que 5,0 mm entre D0 e D7; (2 sua retirada incrementa em 1,7 vezes o crescimento folicular do D7 ao D9; (3 pode contribuir para a ovulação de folículos maiores e, em tese, para maior formação de tecido luteínico; (4 não promove ovulação precoce após o Ovsynch; (5 não eleva as taxas de concepção após sincronização de fêmeas cíclicas e com bom escore corporal, devendo ser avaliada para uso em fêmeas acíclicas ou com ECC mais baixo.The aim of this study was to evaluate the effect of the association of medroxyprogesterone with the Ovsynch protocol on the follicular growth, ovulation, and conception rate of buffaloes in the Eastern Amazon (Tracuateua-PA. Twenty-seven cyclic, non-lactating females (G1 n=14 and G2 n=13 with a 3.5 BCS were synchronized with the Ovsynch. Animals from G2 received a supplementation of 60 mg of medroxyprogesterone from D0 to D7 (D0=starting day of treatment. Ultrasonography evaluation on ovaries was realized on Days 0, 7, 9, and 10. A

  2. Morfologia, ultraestrutura e morfometria do tegumento da paca (Cuniculus paca Linnaeus, 1766 criada em cativeiro

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    José G.M.P. Isola

    2013-05-01

    Full Text Available Considerando-se a falta de informações detalhadas sobre a morfologia da paca, sendo o segundo maior roedor da fauna brasileira, de excelente qualidade de carne e, dada a importância do tegumento comum, inclusive para o bom manejo em cativeiro, e até mesmo estudos como uma opção de animal de experimentação, descreveu-se a morfologia, morfometria e a ultraestrutura da pele de oito pacas (Cuniculus paca machos e fêmeas, mediante a análise comparativa de segmentos cutâneos das regiões cervical, dorsal e medial do carpo. Observaram-se macroscopicamente as características da pelagem. Parte dos segmentos das regiões cutâneas foi analisada à microscopia de luz e parte, à microscopia eletrônica de varredura. Mensuraram-se as espessuras da derme, epiderme, camada córnea, perfis das fibras de colágeno da derme reticular e a área dos perfis das células das glândulas sebáceas repletas. Analisaram-se os resultados pela estatística descritiva e teste "T" (p<0,001. A coloração da pelagem da paca é castanho avermelhado com cerdas organizadas em grupos. A arquitetura da cútis e os anexos cutâneos se assemelham aos dos mamíferos em geral, embora haja ausência de glândulas sudoríparas. De acordo com a analise morfométrica, pôde-se inferir que a arquitetura da cútis de pacas machos e fêmeas apresenta diferenças quando comparada entre os sexos e também em um mesmo animal, diferenciando-se entre diversas regiões corpóreas.

  3. INTESTINAL MICROBIOTE IN AGOUTIS BRED IN CAPTIVITY MICROBIOTA INTESTINAL EM CUTIAS CRIADAS EM CATIVEIRO

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    LEANDRO LUÍS MARTINS

    2009-07-01

    Full Text Available Intestines are colonized by microorganisms, which vary according to the species and the intestinal region. Thus, due the lack of information about this subject in literature, this research was developed for better knowing some aspects of the intestinal microbiology of the agouti, considered one of the biggest Brazilian wild rodents. Three males and three not pregnant adult females, belonging to the Catanduva City Zoo, SP, went through laparotomy after anesthesia. Samples were collected with sterile swab in the medium thirds of the jejune, cecum and greater colon after enterotomy. They were cultivated in Sabouraud agar and in TSA (triptone-soy agar. In the jejune, the growth of Staphylococcus aureus and Escherichia coli was of 83.33%, Salmonella spp. of 66.67% and Clostridium spp. of 50.00% of the samples. In the cecum, they were identified Staphylococcus aureus in 33.33%, Escherichia coli, Salmonella spp. and Clostridium spp. in 100% and Streptococcus spp. in 16.67% of the samples were identified. In the greater colon, the growth of Salmonella spp., Escherichia coli and Clostridium spp. in 66.67%, Staphylococcus aureus in 83.33%, and Streptococcus spp. in 16.67% of the samples was observed, showing the occurrence of variations in the bacteriae proportion according to the intestinal region. 

    KEY WORDS: Dasyprocta azarae, intestines, microbiote.

    Os intestinos são colonizados por microorganismos, variáveis de acordo com a espécie e região intestinal. Assim, em virtude da ausência de informações sobre esse assunto na literatura, esta pesquisa foi desenvolvida para melhor se conhecer alguns aspectos da microbiota intestinal da cutia, um dos maiores roedores selvagens brasileiros. Três machos e três fêmeas adultas vazias, pertencentes ao Zoológico Municipal de Catanduva, SP, foram submetidos a laparotomia mediante anestesia. Amostras com swab estéril foram coletadas nos terços médios do jejuno, ceco e cólon maior, após enterotomia, sendo semeadas em ágar- Sabouraud e em ágar-triptona-soja (TSA. No jejuno, houve o crescimento de Staphylococcus aureus e Escherichia coli em 83,33%, Salmonella spp. em 66,67% e Clostridium spp. em 50,00% das amostras. No ceco, identificaram-se Staphylococcus aureus em 33,33%, Escherichia coli, Salmonella spp. e Clostridium spp. em 100% e Streptococcus spp.  em 16,67% das amostras. No cólon maior, houve o crescimento de Salmonella spp., Escherichia coli e Clostridium spp. em 66,67%, Staphylococcus aureus em 83,33%, e Streptococcus spp. em 16,67% das amostras, mostrando a ocorrência de variações na proporção bacteriana de acordo com a região intestinal.

    PALAVRAS-CHAVES: Dasyprocta azarae, intestinos, microbiota.

  4. Estrutura, ultraestrutura e morfometria da veia cava de paca (Cuniculus paca Linnaeus, 1766 criada em cativeiro

    Directory of Open Access Journals (Sweden)

    Sérgio Pinter Garcia Filho

    2013-05-01

    Full Text Available A paca (Cuniculus paca é o segundo maior roedor da fauna brasileira. Apresenta carne de excelente qualidade, o que incentiva a criação comercial. Além disso, este animal pode tornar-se uma opção válida em experimentação embora poucas sejam as informações detalhadas sobre sua morfologia. Assim, objetivou-se descrever a morfologia, morfometria e ultraestrutura de segmentos das porções cranial e caudal da veia cava de quatro pacas (Cuniculus paca adultas excedentes do plantel do Setor de Animais Silvestres do Departamento de Zootecnia da FCAV-Unesp. Os segmentos venosos foram analisados à microscopia de luz e à microscopia eletrônica de varredura. Foram mensuradas as espessuras do complexo formado pelas túnicas íntima e média, além da túnica adventícia e analisou-se os resultados pela estatística descritiva, teste "T" pareado (p<0,05. Em relação à espessura das túnicas estudadas, comprovou-se que os valores da espessura das túnicas íntima, média e adventícia, para todos os animais, foram significativamente maiores no segmento cranial. As camadas das paredes dos vasos apresentaram variações entre si quanto à estrutura e espessura, supostamente devido a uma adaptação à exigência funcional.

  5. The Medical Biology of Brazilian Calliphoridae: Mechanism for Disease Transmission

    Science.gov (United States)

    1985-01-01

    well to a consideration of the myiasis producers C. hominivorax, Chrysomya albiceps, and Dermatobia hominis . The latter is a serious pest of people...The habits, distribution, % and life cycles of the majority of these flies are largely unknown. These facts are neces- sary to (1) identify the...distribution, and life cycles of the majority of these flies are largely unknown. These facts are necessary to (1) identify the potentially important vectors

  6. Mass rearing of Lucilia sericata Meigen (Diptera:Calliphoridae)

    Institute of Scientific and Technical Information of China (English)

    Firoozfar; F; Moosa-Kazemi; H; Baniardalani; M; Abolhassani; M; Khoobdel; M; Rafinejd; J

    2011-01-01

    Objective:To carry out an experimental study with the main objective of mass rearing of sheep flies(Lucilia sericata).Methods:Hand collection and beef- or cattle liver-baited net traps were used for field fly sampling from April,2010 to November,2010.The samples collected from different places were placed in properly labeled tubes and sent to the Entomology Laboratory. Since maggot identification is important in inducing mortality,they were kept under insectary condition to develop to adult stage and identified using systematic keys.Results:A total of 218 flies were collected in three rounds of sampling from the field of Tehran and Karaj Counties.In the first generation,433 flies including 135(31.17%) male,and 298(68.82%) female were yielded. The female/male of parent ratio was calculated as 1.72 in Tehran and in Karaj areas,whereas it was 2,20%and 1.81%,respectively in Fl and F2 generations,respectively.Conclusions:During this study,the mass rearing of sheep blow fly has been established at the School of Public Health, Tehran University of Medical Sciences and can be used for producing flies for maggot therapy.

  7. Mass rearing of Lucilia sericata Meigen (Diptera:Calliphoridae)

    Institute of Scientific and Technical Information of China (English)

    Firoozfar F; Moosa-Kazemi H; Baniardalani M; Abolhassani M; Khoobdel M; Rafinejd J

    2011-01-01

    Objective: To carry out an experimental study with the main objective of mass rearing of sheep flies (Lucilia sericata). Methods: Hand collection and beef- or cattle liver-baited net traps were used for field fly sampling from April, 2010 to November, 2010. The samples collected from different places were placed in properly labeled tubes and sent to the Entomology Laboratory. Since maggot identification is important in inducing mortality, they were kept under insectary condition to develop to adult stage and identified using systematic keys. Results: A total of 218 flies were collected in three rounds of sampling from the field of Tehran and Karaj Counties. In the first generation, 433 flies including 135 (31.17%) male, and 298 (68.82%) female were yielded. The female/male of parent ratio was calculated as 1.72 in Tehran and in Karaj areas, whereas it was 2.20% and 1.81%, respectively in F1 and F2 generations, respectively. Conclusions: During this study, the mass rearing of sheep blow fly has been established at the School of Public Health, Tehran University of Medical Sciences and can be used for producing flies for maggot therapy.

  8. Attraction and Oviposition of Lucilia eximia (Diptera: Calliphoridae) to Resources Colonized by the Invasive Competitor Chrysomya albiceps (Diptera: Calliphoridae).

    Science.gov (United States)

    Spindola, Aline F; Zheng, Le; Tomberlin, Jeffery K; Thyssen, Patricia J

    2016-10-15

    The present study aimed to determine if the presence of immatures of the invasive blow fly species Chrysomya albiceps (Wiedemann) influences the adult behavior of the native species Lucilia eximia (Wiedemann) in Brazil. The level of attraction and oviposition by the native species was assessed in a dual-choice assay. The evaluation was based on sex and stage of ovarian development of L. eximia adults to a resource not colonized (NCR) or colonized (RPC) with eggs, different instars, or densities of C. albiceps A significant difference in attraction was observed based on sex and stages of ovarian development. Males and nongravid females were more attracted to RPC, whereas gravid females preferred NCR. Moreover, males exhibited the lowest response in all assays among the three sex categories examined. In general, adults preferably oviposited on NCR rather than RPC. Also, between the eggs and second instar treatments, L. eximia laid more eggs on RPC with eggs than second instars (predatory stage). Lucilia eximia attraction to second-instar C. albiceps at different densities was marginally significant. Overall, results indicate the invasive species, C. albiceps, is impacting the behavior of the native blow fly, L. eximia, with regards to its attraction and colonization of vertebrate carrion, which could explain why native blow fly populations have significantly decreased since the introduction of C. albiceps.

  9. Del despertar de la criada a la criada despierta. Reflexión en torno a la écfrasis y el valor de la imagen en un relato de Daniel Briguet

    Directory of Open Access Journals (Sweden)

    Lucía Campanella

    2015-07-01

    Full Text Available This paper analyses the ekphrasis – in its double meaning of “vivid description” and “description of a work of art” – in “El despertar de la criada”, a Daniel Briguet's short story (Rosario, Homo Sapiens, 2010. Through close reading of both the story and the painting (Le lever de la bonne, Eduardo Sívori, 1887 we will study the change and the permanence in the representation of domesticity and its protagonists in the XIXth - XXIth centuries.

  10. A geração criada por mulheres: o pai e o masculino no Clube da Luta

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    Charles Elias Lang

    2011-01-01

    Full Text Available El presente trabajo discute cuestiones relativas a la paternidad y lo masculino. Freud empezó su práctica clínica en un momento marcado por un modelo particular de família, en lo cual existia un discurso que delineaba la masculinidad y la paternidad y estas se ligaban al discurso religioso. En el discurso religioso, había un modelo consistente de lo que se esperaba y un lugar determinado para el hombre y para el padre, en la família y en la sociedad. Hasta 1938, Lacan ha resaltado la degradación del papel de padre, y há caracterizado nuestro tiempo como marcado por la declinación de la función paterna. Partiendo de la idea que la crisis contemporánea tiene como punto basilar la declinación de la función paterna, se hace necesário investigar la diferencia entre los conceptos que son utilizados en esta via, como los conceptos de imagen, imago, papel y función paternas. Además, buscamos alcanzar el núcleo de nuestra investigación verificando el modo como son trabajadas la cuestiones relativas a la paternidad, lo masculino y lo viril en la película El club de la pelea, de 1999. La película fue basada en la novela homónima escrita por Chuck Palahniuk y publicada em 1996, en lo cual el protagonista narra su historia y los eventos que llevaram a criación de Clubs de pelea.

  11. Análise qualitativa do estabelecimento da espermatogênese em cutias (Dasyprocta aguti criadas em cativeiros

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    Antônio Chaves de Assis-Neto

    2003-01-01

    Full Text Available A determinação do estabelecimento da puberdade é bastante estudada em animais domésticos e roedores, no entanto, são escassas as pesquisas com a finalidade de estabelecer parâmetros para a biologia reprodutiva em cutias. Foram utilizadas 31 cutias machos da espécie Dasyprocta agouti, oriundas da Universidade Federal do Piauí, Estado do Piauí, e da Escola Superior de Agricultura de Mossoró, Estado do Rio Grande do Norte. Imediatamente após a orquiectomia foram retirados fragmentos e estes foram processados histologicamente, os tecidos foram corados com hematoxilina-eosina e analisou-se os parâmetros seguintes: aspectos de luminação dos túbulos seminíferos; presença de espermatócitos primários; presença de espermátides e formação dos primeiros estágios do ciclo do epitélio seminífero (CES segundo o método da morfologia tubular. O período desde o nascimento até os cinco meses de idade correspondeu à fase impúbere; dos seis aos oito meses de idade a fase de transição da pré-puberdade à puberdade; dos nove aos dez meses de idade à fase da puberdade; e dos doze aos quartoze meses de idade à fase da pós-puberdade. A puberdade da cutia (Dasyprocta aguti, ocorreu em animais a partir dos sete meses de idade, e o estabelecimento da puberdade foi constatado em todos os animais estudados aos nove meses de idade.

  12. Análise da Metodologia Criada por Miguel Cifuentes Referente à Capacidade de Carga Turística

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    Maurício Delgado

    2013-02-01

    Full Text Available Muitos pesquisadores do setor turístico têm utilizado a metodologia da capacidade de carga. A idéia básica é estabelecer um número máximo de visitantes em uma determinada localidade, com o objetivo de minimizar os impactos provocados pela visitação. Entretanto, esta metodologia está incompleta ao atrelar os impactos da visitação apenas ao número de visitantes, deixando de considerar aspectos subjetivos e qualitativos relacionados ao turismo. Esta pesquisa demonstra a falta de cientificidade desta metodologia e salienta que limitar o número de visitantes é apenas um dos métodos de controle dos impactos provocados pelo turismo. Many researchers in the tourism sector have used the methodology of carrying capacity. The basic principie is to establish a maximum number of visitors in some place in order to minimize the impact of tourism. However, this methodology is incomplete as it only relates the impacts of tourist activity to the number of visitors, without considering some of the subjective and qualitative aspects of the effects of tourism. This research demonstrates the unscientific nature of this methodology and stresses that limiting the number of visitors to a locality is only one method to control the impacts of tourism.

  13. O processo empreendedor em empresas criadas por necessidade The entrepreneurial process in firms created out of necessity

    Directory of Open Access Journals (Sweden)

    Janis Elisa Ruppenthal

    2012-01-01

    Full Text Available O ofício de criar empresas é inerente aos empreendedores. Quando a motivação é a necessidade, o risco de insucessos aumenta muito. Nesse trabalho, busca-se conhecer, compreender e compartilhar os aprendizados de um grupo de empreendedores que passaram por essa situação e obtiveram sucesso. A partir do ponto de vista teórico dos autores que tratam do pragmatismo dos processos empreendedoriais, o estudo de caso buscou, no decurso das entrevistas com os empresários, a relação entre as teorias e práticas. Instituída a relação, sugere-se um modelo de atuação empreendedora que possa orientar outros empresários em situação semelhante. Ao final, observou-se que ter seu próprio negócio os motivou e a atenção aos feedbacks os estimulou para as mudanças. O elevado grau de coerência entre o empreendedor, seu negócio e o ambiente favoreceram a situação. Enquanto desenvolviam experiências empreendedoriais, essas pessoas, influenciadas por diferentes modos de atuação, alteraram os seus, levando-as a desenvolver o processo empreendedor. Diante disso, pode-se dizer que suas atitudes catalisaram as mudanças, caracterizadas pelo elevado grau de assertividade.Creating firms is an inherent entrepreneurial spirit. When necessity is the motivation, the risk of failure is high. The objective of this study is to understand and share the learnings and experiences of an entrepreneurial group that was faced with this challenging situation and reached success. From the theoretical point of view of the authors who dealt with the entrepreneurial process, the case study aimed to establish a relationship between theory and practice through interviews with entrepreneurs. Once this relationship was established, a model of entrepreneurial action that can guide other entrepreneurs in similar situations was suggested. It was observed that the idea of having their own business motivated the entrepreneurs and the feedback received stimulated changes. Prudent entrepreneurship, the business created, and the environment favored this situation. While developing entrepreneurial experiences, these entrepreneurs, influenced by different types and styles of management, changed their own management approach and developed the entrepreneurial process. This behavior speeded up changes leading to high levels of accomplishment.

  14. AmbiÃncia e bem-estar de ovelhas criadas em sistema agrossilvipastoril no semiÃrido cearense.

    OpenAIRE

    IsmÃnia FranÃa de Brito

    2012-01-01

    This study aimed to establish the physiological parameters of ewes reared in agrosilvopasture system in the northeast semiarid and hence assess environmental conditions offered by the system. Thus, 22 crossbred ewes were selected and evaluated during the dry and rainy seasons, considering the following physiological parameters: heart rate (HR), respiratory rate (RR), surface temperature (ST) and rectal temperature (RT) during shifts morning and afternoon. Six mini-stations and meteorological ...

  15. Lucilia eximia (Diptera: Calliphoridae, a new alternative for maggot therapy. Case series report Lucilia eximia (Diptera: Calliphoridae, una nueva alternativa para la terapia larval

    Directory of Open Access Journals (Sweden)

    Silvia Emelia Herrera Higuita

    2010-05-01

    Full Text Available

    Larval therapy is used in the treatment of infected chronic wounds by allowing the removal of necrotic tissue, which induces the formation of granular tissue and the growth of healthy skin.

     

    Considering the increasing prevalence of antibiotic resistance, this type of therapy may be an effective alternative in the management of infected chronic wounds. In this article we report the use of maggot therapy using the Lucilia eximia species in 42 patients with chronic skin wounds associated to different pathologies including: venous and arterial ulcers, diabetic foot, sickle cell disease, vasculopathy, elephantiasis, Berger disease, pyoderma gangrenosum (PG, traumatic wounds, erysipelas, and hospital acquired infections. Four cases are depicted photographically.

    La terapia larval es utilizada desde los años 30 del siglo pasado para la remoción del tejido necrótico en el tratamiento de úlceras crónicas infectadas logrando con ello promover la formación de tejido granuloso para el crecimiento de piel sana; especialmente a partir de la aparición de la resistencia a los antibioticos se la reconoce como una alternativa eficaz. Se presentan los resultados de la aplicación de la terapia larval en 42 pacientes con úlceras de diferentes orígenes, utilizando, como una nueva alternativa en larviterapia, la especie Lucilia eximia. Se presenta documentación fotografica de la aplicacion de esta terapia en cuatro casos.

     

     

  16. Lucilia eximia (Diptera: Calliphoridae), a new alternative for maggot therapy. Case series report Lucilia eximia (Diptera: Calliphoridae), una nueva alternativa para la terapia larval

    OpenAIRE

    Silvia Emelia Herrera Higuita; Carolina Rivera Álvarez; Marta Isabel Wolff Echeverri; Mónica María Escobar Franco; Juan Carlos Wolff Idárraga

    2010-01-01

    Larval therapy is used in the treatment of infected chronic wounds by allowing the removal of necrotic tissue, which induces the formation of granular tissue and the growth of healthy skin.

    Curva de sobrevivência e estimativa de entropia em Lucilia cuprina (Diptera, Calliphoridae Survivorship curve and estimate of entropy in Lucilia cuprina (Diptera, Calliphoridae

    Directory of Open Access Journals (Sweden)

    Francely M. Fernandes

    2003-09-01

    Full Text Available Lucilia cuprina (Wiedemann, 1830 is a cosmopolite blowfly species of medical and veterinary importance because it produces myiasis, mainly in ovine. In order to evaluate the demographic characteristics of this species, survivorship curves for 327 adult males and 323 adult females, from generation F1 maintained under experimental conditions, were obtained. Entropy was utilized as the estimator of the survival pattern to quantify the mortality distribution of individuals as a function of age. The entropy values 0.216 (males and 0.303 (females were obtained. These results denote that, considering the survivorship interval until the death of the last individual for each sex, the males present a tendency of mortality in more advanced age intervals, in comparison with the females.

  17. First Record of Chrysomya rufifacies (Macquart (Diptera, Calliphoridae in Southeastern Brazil

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    Antonia Ribeiro

    2013-12-01

    Resumo. A presente nota relata o primeiro registro da espécie Chrysomya rufifacies (Macquart, no Sudeste do Brasil, no Município de Seropédica, Rio de Janeiro. As coletas foram realizadas com auxílio de armadilhas para dípteros e a isca utilizada foi peixe fresco.

  18. Microsatellite markers for population genetic studies of the blowfly Chrysomya putoria (Diptera: Calliphoridae

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    Rosangela Aparecida Rodrigues

    2009-11-01

    Full Text Available The investigation of the genetic variation and population structure of Chrysomya species is of great interest for both basic and applied research. However, very limited genetic information is available for this genus across its geographical distribution. Here, we describe 12 polymorphic microsatellite loci isolated from Chrysomya putoria with expected heterozygosities ranging from 0.1402-0.8312. These markers are of potential applied interest for forensic entomologists and for the characterisation of the genetic structure of C. putoria from recently colonised regions, with great promise for understanding the colonisation dynamics and spread of the genus Chrysomya in the New World.

  19. Distinction among the puparia of three blowfly species (Diptera: Calliphoridae frequently found on unburied corpses

    Directory of Open Access Journals (Sweden)

    JA Amorim

    2001-08-01

    Full Text Available Calliphorid larvae are important in the decomposition of carrion. Since these larvae are present in the primary stages of succession on carcasses, they may be important indicators of death time and the movement of corpses in homicide investigations. In this study we examined the morphological differences among puparia of Chrysomya megacephala, C. putoria and Cochliomyia macellaria. Puparia of the three species (N=30, each were obtained from the F2 generation bred in culture medium at 25° C, and 60% relative humidity on a 12 h photoperiod. The interspecific differences found were related to the conspicuousness of six tubercles located in the region near the posterior spiracles and to the distance between the two peritrema involving the spiracles. The latter were (mean ± SD 15.2 ± 3.1 mum for C. megacephala, 18.8 ± 2.8 mum for C. putoria and 16.5 ± 3.5 mum for C. macellaria. The results of the present study may be useful in forensic entomology.

  1. Nasal Nosocomial Myiasis Infection Caused by Chrysomya bezziana (Diptera: Calliphoridae Following the Septicemia: A Case Report

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    Seyed Farzin MIRCHERAGHI

    2016-10-01

    Full Text Available A 74 yr old woman from Gonabad, southern part of Khorasan Razavi Province of Iran was admitted to a Hospital of Gonabad, because of respiratory distress, exertional dyspnea and fever. Close contact with domes­tic animals, history of chronic obstructive pulmonary disease (COPD, and completely resolved pulmonary tuberculosis (TB in remote past, were nota­ble parts of her past medical history. Due to clinical, paraclinical and radio­graphic findings and because of recent hospitalization, she was admitted to internal medicine ward with the diagnosis of health care associated pneumo­nia (HCAP. Despite the application of broad-spectrum antibiotics and ap­propriate supportive care, she had a poor response to the treatment. During the daily visit in Intensive Care Unit (ICU, numerous white larvae were de­tected in both nostrils. Further investigation of oropharynx and tracheal tube aspiration, showed no more larvae in mentioned parts. An hour later, nasal spontaneous bleeding occurred. Otorhinolaryngology consultation was per­formed and led to surgical procedure. In ENT examination, there were nu­merous larvae and massive clot formation in both inferior meatuses and distal nasal septum perforation. Thirty-seven extracted larvae were transferred to Medical Entomology lab by vial 70% ethanol and 5 live larvae for rearing. After pre­cise investigation by aid of light microscopy, the larvae were identified as Chrysomya bezziana. Due to discovered 2nd larvae stage and duration of hospitalization, this infestation was identified as nasal myiasis.

  2. "Epidermal Streaming" and associated phenomena displayed by Larvae of Chrysomyia marginalis (Wd. (Diptera: CALLIPHORIDAE at carcasses

    Directory of Open Access Journals (Sweden)

    L.E.O Braack

    1984-12-01

    Full Text Available Competition with vertebrate scavengers for carrion necessitates blow-fly larvae to make rapid and effective use of dead animals. Blow-fly eggs are deposited in shaded, concealed positions at a carcass, and the larvae need to enter the body to feed on muscle and other soft tissues as soon as possible after eclosion. They do so by digesting away the skin with enzymes, but this is often preceded by an exploratory phase in which larvae migrate over the carcass to find wounds by which they are able to gain more rapid entry into the body. Epidermal streaming is one method of performing this exploratory phase, and involves movement by small larvae below the superficial outermost layer of skin. Several possible benefits of this behaviour are discussed.

  3. Description of the male of Laneella perisi (Mariluis) (Diptera: Calliphoridae) n. comb.

    Science.gov (United States)

    Wolff, M; Ramos-Pastrana, Y; Pujol-Luz, J R

    2013-02-01

    The male Laneella perisi (Mariluis) n. comb. is described based on specimens collected in the Cordillera Oriental (1,370-1,450 m asl), Florencia-Suaza, Caquetá, Colombia. A key to separate the two species of the genus Laneella and illustrations of the male genitalia and female abdomen, terminalia, and spermatheca are also presented.

  4. Development of the oriental latrine fly, Chrysomya megacephala (Diptera: Calliphoridae), at five constant temperatures

    Science.gov (United States)

    Gruner, S. V.; Slone, D.H.; Capinera, J.L.; Turco, M. P.

    2017-01-01

    Chrysomya megacephala (Fabricius) is a forensically important fly that is found throughout the tropics and subtropics. We calculated the accumulated development time and transition points for each life stage from eclosion to adult emergence at five constant temperatures: 15, 20, 25, 30, and 35 °C. For each transition, the 10th, 50th, and 90th percentiles were calculated with a logistic linear model. The mean transition times and % survivorship were determined directly from the raw laboratory data. Development times of C. megacephala were compared with that of two other closely related species, Chrysomya rufifacies (Macquart) and Phormia regina (Meigen). Ambient and larval mass temperatures were collected from field studies conducted from 2001–2004. Field study data indicated that adult fly activity was reduced at lower ambient temperatures, but once a larval mass was established, heat generation occurred. These development times and durations can be used for estimation of a postmortem interval (PMI).

  5. A fresh liver agar substrate for rearing small numbers of forensically important blow flies (Diptera: Calliphoridae)

    Science.gov (United States)

    Gruner, Susan V.; Slone, Daniel H.

    2014-01-01

    Forensically important calliphorids can be reared on a mixture of beef liver and agar. Small pieces of meat, especially fresh or frozen beef liver, will desiccate in 2–6 h, but this simple-to-make feeding substrate remains moist for at least 12 h at 25 and 30°C without desiccation, even in small (5 g) amounts. We determined the survivorship of small numbers of Chrysomya megacephala (F.) (first-instar larvae to adult eclosion) raised on 5 g of liver agar and fresh beef liver. We found that all larvae raised on 5 g of liver died due to desiccation, but survivorship on 5 g of liver agar was equivalent to that on larger (50 g) pieces of either liver agar or beef liver.

  6. Focus stacking technique in identification of forensically important Chrysomya species (Diptera: Calliphoridae

    Directory of Open Access Journals (Sweden)

    Noha A. Elleboudy

    2016-09-01

    Recommendations: Further studies on the blowfly species that occur in Egypt and documentation of their key for identification are recommended to facilitate the diverse applications of these important insects in forensic investigations.

  7. A new DNA extraction protocol for screwworm fly Cochliomyia species. (Diptera: Calliphoridae.

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    Gustavo eEcheverría-Fonseca

    2015-01-01

    Full Text Available Modifications to the DNA isolation protocol from Cochliomyia spp., are suggested based on the Chelex® 100 reactive. To apply the sterile insect technique (SIT program it is necessary to study the molecular variations of endemic populations with efficient, fast and low costs techniques. The test samples were collected in the Pichincha province of Ecuador. The isolation protocol had 3 steps: a pretreatment (optional, b mechanic and chemical lysis, c two incubations; then the supernatant were separated by centrifugation. Furthermore, variations in concentrations of magnesium chloride present in the master mix were evaluated. Results showed a high efficiency in isolation with approximately 1.20 hours of manipulation (without pretreatment. Additionally, the quality of the amplicon that was visualized on 2% agarose (w/v showed that the magnesium chloride concentration was influential in the PCR reaction mix.

  8. Investigating the potential of fluorescent fingerprint powders as a marker for blow fly larvae (Diptera: calliphoridae).

    Science.gov (United States)

    Rosati, Jennifer Y; Robinson, Scott D; Devine, Richard

    2015-05-01

    Four fluorescent fingerprint powders (RedWop(™) , GreenWop(™) , Basic Yellow(™) , and Yellow Powder(™) ) were evaluated as a marker for blow fly larvae. Administration methods included ingestion (high vs. low concentration) or topical. Ingestion of high concentrations of Basic Yellow(™) and RedWop(™) caused higher larval mortality. Basic Yellow(™) delayed development and adult emergence while RedWop(™) and Yellow Powder(™) had a significant effect on particular stages of development, however, emergence time was not altered. Optimal administration is through ingestion at low concentration levels (powders was 450 nm. This research can aid in investigative training to increase visibility of larval and pupal blow flies. It can also be used in entomological studies to differentiate between larval blow flies (or other dipteran) species or individuals to further understand complex interactions and behavior during larval development.

  9. A review of bacterial interactions with blow flies (Diptera: Calliphoridae) of medical, veterinary, and forensic importance

    Science.gov (United States)

    Blow flies are commonly associated with decomposing material. In most cases, the larvae are found feeding on decomposing vertebrate remains. However, some species have specialized to feed on living tissue or can survive on other alternate resources like feces. Because of their affiliation with su...

  10. Efeito de quatro anitibioticos sobre larvas de Chrysomya putoria (Wiedewann) (Diptera: Calliphoridae) utilizadas em bioterapia

    OpenAIRE

    Maria Lucia Marcucci Torres

    2005-01-01

    Resumo: A bioterapia ou terapia de desbridamento larval, é a utilização de larvas vivas de moscas varejeiras para tratamento de feridas de difícil cicatrização. Este tipo de tratamento foi descoberto acidentalmente em condições de campos de batalha, teve seu auge nas décadas de 1930 e 1940 e atualmente tem sido utilizado em muitos países. As larvas aplicadas na ferida promovem a cura por meio de vários mecanismos, como liquefação do tecido necrosado, remoção das bactérias, secreção de substân...

  11. The effect of two barbiturates on the development of three calliphoridae species (Diptera) of forensic importance

    OpenAIRE

    2008-01-01

    Resumo: A Entomologia Forense utiliza dados de desenvolvimento e aspectos ecológicos de insetos necrófagos com o objetivo de auxiliar as investigações criminais, entre outros, no cálculo da estimativa do intervalo pós-morte (IPM). Igualmente, pode-se caracterizar a causa da morte quando essas drogas são detectadas em insetos imaturos que se alimentam dos corpos em decomposição. Além disso, os insetos podem servir como uma ferramenta alternativa para análises toxicológicas na ausência de tecid...

  12. Induced Cannibalism in Experimental Populations of the Forensic Indicator Chrysomya putoria Wiedemann (Diptera: Calliphoridae).

    Science.gov (United States)

    Botteon, V W; Fernandes, F S; Godoy, W A C

    2016-04-01

    We analyzed the behavior of third-instars of Chrysomya putoria as potential cannibals in experimental populations. Cannibalism rates were evaluated in three settings observed for 3, 6, 9, and 24 h, placing injured and uninjured larvae of C. putoria together. Our data heavily support that C. putoria larvae behave as cannibals when induced by a wound in another larva, and also after starving for 24 h. The probability of cannibalism increased as a function of time, both in no-choice and in choice experiments evidencing that time is a determining factor for cannibalism induction in C. putoria. However, the treatment combining injured with uninjured larvae showed the highest probability of cannibalism. These results suggest that C. putoria larvae may cannibalize under scarcity of food over long time or the presence of injured larvae. This study is useful to understand the behavior of C. putoria feeding on ephemeral substrates such as carrion or corpses and brings relevant and significant contribution to population ecology of blowflies and also forensic entomology.

  13. Differentiation between Lucilia cuprina and Hemipyrellia ligurriens (Diptera: Calliphoridae) larvae for use in forensic entomology applications.

    Science.gov (United States)

    Sukontason, Kom; Sribanditmongkol, Pongruk; Ngoen-klan, Ratchadawan; Klong-klaew, Tunwadee; Moophayak, Kittikhun; Sukontason, Kabkaew L

    2010-02-01

    Lucilia cuprina and Hemipyrellia ligurriens are forensically important blow flies in many countries. The immature stage, in particular the third instar, of both species are generally similar in morphological appearance. In this study, the use of three main characteristics, the posterior spiracle, the dorsal spines between the first and second thoracic segments, and the cephalopharyngeal skeleton, allows for identification of both fly species, with the number of papillae on anterior spiracle being a supportive characteristic in some cases. Comparison of these characteristics is illustrated in detail.

  14. Morphology of immature stages of Hemipyrellia ligurriens (Wiedemann) (Diptera: Calliphoridae) for use in forensic entomology applications.

    Science.gov (United States)

    Sukontason, Kabkaew L; Sribanditmongkol, Pongruk; Chaiwong, Tarinee; Vogtsberger, Roy C; Piangjai, Somsak; Sukontason, Kom

    2008-09-01

    In forensic investigations, all immature stages of flies (egg, larvae, and puparium) can serve as entomological evidence at death scenes. These insects are primarily used to estimate the post mortem interval (PMI), but can also be involved in the analysis of toxic substances, determining manner of death, and in indicating relocation of a corpse in homicide cases. In this study, we present the morphology of the egg, larvae, and puparium of Hemipyrellia ligurriens, a blow fly species of forensic importance in Thailand. Examination was conducted using scanning electron microscopy (SEM). The egg stage was found to display a relatively wide plastron region (or median hatch line area) that spans almost the entire length of the egg. The median hatch line is oriented in an upright position. External chorionic sculpture of the egg is present in a hexagonal pattern whose reticular boundaries are slightly elevated. In the larval stages, the most prominent morphological changes were detected upon comparison of the first to the second instar; whereas, the differences between second and third instar larvae were less obvious outside of the increase in number of posterior spiracular slits. Most of the major differences involve body size and structure of the anterior and posterior spiracles. Each anterior spiracle in both the second and third instars projects five to seven papillae apically. Each posterior spiracular disc of a third instar exhibits a complete peritreme, three spiracular slits, and a prominent button that is ventromedially located. The puparium is coarctate and features a clustered bubble membrane comprised of approximately 57 mammillate structures positioned dorsolaterally on each side of the first abdominal segment in young puparia. This feature is replaced by short, tubular respiratory horns in aged puparia. This study provides more detailed exposure of important morphological features that can be used for accurate identification of immature stages of H. ligurriens. Information presented can aid in forensic investigations involving this fly species.

  15. Postfeeding radial dispersal in larvae of Chrysomya albiceps (Diptera: Calliphoridae): implications for forensic entomology.

    Science.gov (United States)

    Gomes, Leonardo; Zuben, Claudio José Von

    2005-12-01

    Blowflies utilize discrete and ephemeral breeding sites for larval nutrition. After the exhaustion of food, larvae begin dispersing in search of sites to pupate or additional food sources, a process referred as postfeeding larval dispersal. Some of the most important aspects of this process were investigated in the blowfly Chrysomya albiceps, employing a circular arena to allow radial dispersion of larvae from the center. The results showed a positive correlation between burial depth and distance, and a negative correlation between distance and pupal weight. These results can be used in forensic entomology for the postmortem interval estimation of human corpses in medico-criminal investigations.

  16. Localization of the glycoprotein Cb42 in larvae of the screwworm fly Chrysomya bezziana (Diptera: Calliphoridae

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    Creig Eisemanni

    2000-10-01

    Full Text Available The glycoprotein Cb-peritrophin-42 was localized in third instar larvae of Chrysomya bezziana using primary antibodies raised against a non-glycosylated bacterial recombinant form of this protein. Both immunofluorescent antibody techniques on unfixed whole mounts of gut tissues and immunogold electron microscopy techniques on ultra-thin sections of fixed and embedded tissues were employed. The protein was shown to be exposed over the whole of both surfaces of the peritrophic membrane and to occur throughout its thickness. Immunogold labelling indicated that Cb-peritrophin-42 was expressed in the peritrophic membrane-secreting cells of the cardia, a specialized peritrophic membrane-forming organ situated at the junction of the foregut and midgut. The accessibility of Cb-peritrophin-42 present in intact peritrophic membrane to the primary antibodies used in the immunofluorescent antibody localization indicates that this glycoprotein is a potential molecular target for vaccination of host animals against larvae of Chrysomya bezziana.

  17. Distribution and Persistence of Sterile Screwworms (Diptera: Calliphoridae) Released at the Panama-Colombia Border.

    Science.gov (United States)

    Skoda, Steven R; Phillips, Pamela L; Sagel, Agustin; Chaudhury, Muhammad F

    2017-04-01

    The sterile insect technique is used by the Comisión Panamá - Estados Unidos para la Erradicación y Prevención del Gusano Barrenador del Ganado (COPEG) to maintain a barrier at the border of Panama and Colombia to prevent screwworms, Cochliomyia hominivorax (Coquerel), from South America reinfesting North America. Before studying the distribution and persistence of sterilized, mass-produced screwworms released in the barrier zone, the utility of applying fluorescent dust (∼1.0 mg/fly) to pupae and to newly emerged adults was evaluated to determine the potential effect on fly survival. The flight ability of flies collected from two adult emergence/collection systems (enclosed towers and open chambers) and treated with low (∼0.20 mg/fly) or high (∼1.0 mg/fly) amounts of fluorescent powder was compared. The distribution and persistence of sterile screwworms marked with fluorescent powder (∼0.20 mg/fly), after collection from the same two adult emergence/collection systems, was compared after their release in the barrier zone. The results demonstrated that: 1) fluorescent dust did not negatively affect sterile screwworm longevity or flight ability; 2) no differences were detected between sterile flies collected from the two emergence systems; and 3) sterile screwworms distributed evenly in the barrier zone and persisted for > 6 d. This information was useful in implementing the use of a new sterile fly emergence/collection system and deploying a new strain by COPEG for the barrier zone maintenance program; it will be valuable for evaluating alternative release strategies of sterile screwworms by the eradication and barrier maintenance program. Published by Oxford University Press on behalf of Entomological Society of America 2017. This work is written by US Government employees and is in the public domain in the US.

  18. A fresh liver agar substrate for rearing small numbers of forensically important blow flies (Diptera: Calliphoridae).

    Science.gov (United States)

    Gruner, Susan V; Slone, Daniel H

    2014-05-01

    Forensically important calliphorids can be reared on a mixture of beef liver and agar. Small pieces of meat, especially fresh or frozen beef liver, will desiccate in 2-6 h, but this simple-to-make feeding substrate remains moist for at least 12 h at 25 and 30 degrees C without desiccation, even in small (5 g) amounts. We determined the survivorship of small numbers of Chrysomya megacephala (F.) (first-instar larvae to adult eclosion) raised on 5 g of liver agar and fresh beef liver. We found that all larvae raised on 5 g of liver died due to desiccation, but survivorship on 5 g of liver agar was equivalent to that on larger (50 g) pieces of either liver agar or beef liver.

  19. Ophthalmomyiasis caused by a Phormia sp. (Diptera: Calliphoridae) larva in an enucleated patient.

    Science.gov (United States)

    Kim, Jae-Soo; Kim, Jong-Wan; Lee, Hye-Jung; Lee, In-Yong; Oh, Sang-Ah; Seo, Min

    2011-06-01

    Ophthalmomyiasis rarely occurs worldwide, and has not been reported in Korea. We present here a case of ophthalmomyiasis caused by Phormia sp. fly larva in an enucleated eye of a patient. In June 2010, a 50-year-old man was admitted to Dankook University Hospital for surgical excision of a malignant melanoma located in the right auricular area. He had a clinical history of enucleation of his right eye due to squamous cell carcinoma 5 years ago. During hospitalization, foreign body sensation developed in his right eye, and close examination revealed a fly larva inside the eye, which was evacuated. The larva was proved to be Phormia sp. based on the morphology of the posterior spiracle. Subsequently, no larva was found, and the postoperative course was uneventful without any complaints of further myiasis. This is the first case of ophthalmomyiasis among the literature in Korea, and also the first myiasis case caused by Phormia sp. in Korea.

  20. BIOCHEMISTRY CHARACTERIZATION OF EXCRETION / SECRETION PRODUCT OF Cochliomyia hominivorax LARVAE (DIPTERA : CALLIPHORIDAE

    Directory of Open Access Journals (Sweden)

    Denise Gonçalves Teixeira

    2016-10-01

    Full Text Available The species Cochliomyia hominivorax, also known as screwworm fly, is an obligate parasite of warm- blooded animals and its geographic range extends thoughout South America, except Chile. This fly causes significant economic losses and has great importance in Brazil. Few studies have focused on the excretion and secretion products of this species, and this research aimed to study the enzymes present in the secretion and excretion (E/S products of the three larval instars of C. hominivorax. The E/S profile of proteins was obtained by polyacrylamide gel electrophoresis and proteolytic activity was analyzed using gelatin, azocasein and Na-benzoyl-arginine-nitroanilide as substrates.  In E/S products of the three instars, proteins were detected with an apparent molecular weight ranging between 116 and 20 kDa. In the azocasein assay, at different pH ranges, the major proteolytic activity occurred at pH 7.5 for all larval instars. Assays were performed using the same substrates   in which the samples were treated with the inhibitors Benzamidine, Pepstatin A, 4-(2-Aminoethyl benzenesulfonyl fluoride hydrochloride (AEBSF, N-α-tosyl-L-lysine chloromethyl ketone (TLCK, N-α- tosyl-L-phenylalanine chloromethyl ketone (TPCK, Ethylenediamine tetraacetic acid (EDTA, and Leupeptin-trans-Epoxysuccinyl-leucylamido(4-guanidino butane (E-64. Proteinases present in the E/S product of first larvae instar are mostly serine trypsin and chymotrypsin proteases, whereas for second and third instars serine proteases and aspartyl proteases were predominantly observed. Biochemical characterization of E/S products of all larval stages of C. hominivorax helps to improve the understanding of the physiology and the interaction of this parasite with host tissues. Keywords: Enzyme; fly; myiasis; parasites.

  1. Desempenho e características de carcaça suína de dois cruzamentos de linhagens comerciais criadas em cama sobreposta

    OpenAIRE

    2007-01-01

    Para avaliar o efeito de desempenho zootécnico e rendimento de carcaça comercial de suínos criados em cama sobreposta foram conduzidos três experimentos. No experimento foram utilizados 480 suínos, onde foram acompanhados o desempenho zootécnico entre sexo e cruzamento. Foram utilizados dois cruzamentos de duas linhagens fêmea Dalland com macho PIC 412 - Agroceres e fêmea DanBred com macho PIC 412 - Agroceres, onde foi observados efeitos significativos nos sexos para consumo diário (CD) e sem...

  2. Análise espermática de perdizes (Rhynchotus rufescens criadas em cativeiro e suplementadas com selênio

    Directory of Open Access Journals (Sweden)

    Paola Almeida de Araújo Góes

    2011-10-01

    Full Text Available Due to the commercial importance of the red-winged tinamou (Rhynchotus rufescens, for the past few years, the employment of reproductive biotechnologies has been attempted. Thirty animals were randomly assigned into two groups: control group (no selenium and treatment group (supplemented with 0,2 a 0,8 mg selenium/ 1000 kg ration. Animals were allocated at the FCAV - UNESP/Jaboticabal (2007-2008. Semen collections were performed by digital manipulation and divided in pools of at least 150 µL. After the immediate evaluation of motility, vigour, concentration and morphology, an aliquot of 20 µL was diluted in 300 µL of physiologic solution in order to test acrosome and membrane integrities, which were performed by counting 200 cells for each test. Cells were evaluated as follows: 1 Intact acrosome: lilac acrosome; Non-intact acrosome: pink acrosome; 2 Live cells: non stained; Dead: stained. Data was statistically analysed using the SAS System for Windows. No differences were found between treatment and control groups for volume, motility, vigour, mean number of spermatozoa per animal, concentration, Intact acrosome, Intact membrane. The difference found on midpiece sperm defect (Se = 1,33 ± 0,53 and control = 3,78 ± 0,69, p = 0.0107 may be due to the damages caused by the selenium deficiency to the architecture of the midpiece, which compromises sperm mobility and fertilization capacity.

  3. Erupção dental em pacas (Agouti paca criadas em cativeiro Dental eruption in pacas (Agouti paca bred in captivity

    Directory of Open Access Journals (Sweden)

    F.S. Oliveira

    2007-04-01

    Full Text Available Fez-se o acompanhamento radiográfico mensal da erupção dental de cinco fêmeas e sete machos da espécie Agouti paca durante 30 meses. Os animais foram radiografados mês-a-mês. As pacas nasceram com três dentes em cada hemiarcada dental, um incisivo de crescimento contínuo por toda a vida, um pré-molar decíduo (o único que é decíduo e um molar. No quarto mês houve a erupção do segundo molar. O nivelamento da superfície de oclusão dos dentes inferiores ocorreu no sexto mês e o dos superiores ao redor do nono mês. No 14° mês, houve o início da erupção do terceiro molar e, no 16° mês, a evidenciação radiográfica do pré-molar permanente, ainda com preservação de correspondente decíduo. No 21° mês, ocorreu o nivelamento da superfície de oclusão dos quatro dentes superiores e inferiores. Entre o 22° e o 24° mês, iniciou-se a queda dos pré-molares decíduos inferiores e, entre o 23º e o 24° mês, a dos superiores. Entre o 24º e o 25º mês, a superfície de oclusão dos dentes inferiores estava nivelada, e a dos dentes superiores aconteceu no 27º mês.Five females and seven males of Agouti paca were used to study dental eruption. In a monthly radiographic survey of the dental eruption, during 30 consecutive months, animals were radiographed. Pacas were born with three teeth in each dental hemiarcade, one incisor of continuous growth, one premolar (the only one deciduous and one molar. The second molar erupted in the fourth month. The occlusal surfaces of lower teeth were at the same level in the sixth month and the upper teeth around ninth month. The beginning of the third molar eruption occurred in the 14th month. Perception of the first permanent premolar was around the 16th month, and its correspondent deciduous was still retained. The occlusal surfaces of upper and lower teeth were at the same level in the 21st month. The eruption of the first lower deciduous premolar occurred between the 22nd and 24th month and the upper´s between the 23rd and 24th month. The occlusal surfaces of lower teeth were at the same level between the 24th and 25th month and the upper's on the 27th month.

  4. PESO PÓS-PARTO, FREQÜÊNCIA E PREVALÊNCIA DO TIPO DE PARTO DE CUTIAS (Dasyprocta sp CRIADAS EM CATIVEIRO

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    Maria Acelina Martins de Carvalho

    2006-10-01

    Full Text Available A pesquisa objetivou avaliar parâmetros de desenvolvimento de cutias, buscando fornecer dados para contribuir com a seleção, o melhoramento genético e formas adequadas de manejo para estes animais. Foram observados o peso pós-parto, freqüência e a prevalência do tipo de parto em função da época do ano em 33 fêmeas. Constatou-se que não houve interação entre época do ano e tipo de parto (P>0,05. O peso médio pós-parto foi de 2,24 Kg e 2,39 Kg, para o período de janeiro a dezembro, respectivamente, não apresentando diferença significativa para esta variável (P>0,05. Com relação ao tipo de parto, as cutias com parto simples apresentaram peso médio de 2,32 Kg e as com parto duplo 2,38 Kg, não sendo estes valores significativamente diferentes entre si (P>0,05. Concluiu-se que o peso da cutia fêmea pós-parto, a freqüência e o tipo de parto (simples e duplos não sofrem influência da época do ano e que a cutia em cativeiro se comporta como poliéstrica contínua. PALAVRAS-CHAVE: Cutia, peso pós-parto, prevalência, tipo de parto.

  5. Enfermidades de cutias (Dasyprocta aguti criadas em cativeiro diagnosticadas pelo exame anatomopatológico Diseases of agouti (Dasyprocta aguti raised in captivity diagnosed by pathological examination

    Directory of Open Access Journals (Sweden)

    Jael S. Batista

    2010-06-01

    Full Text Available O exame necroscópico é especialmente útil no diagnóstico de enfermidades em animais silvestres. Em muitas ocasiões, as manifestações clínicas não são características como em animais domésticos, sendo frequente a ocorrência de óbitos em animais assintomáticos. Este trabalho objetivou realizar um estudo retrospectivo sobre as doenças de cutias diagnosticadas pelo Laboratório de Patologia Veterinária da Universidade Federal Rural do Semi-Árido, através do exame anatomopatológico no período de 2006 a 2009. Em 32 cutias submetidas à necropsia, as percentagens das enfermidades diagnosticadas foram: morte perinatal pelo complexo inanição/hipotermia (21,6%, urolitíase obstrutiva (6,24%, distocia (6,24%, obstrução do ceco por areia - sablose (6,24%, intussuscepção (3,20%, fecaloma (3,20% e obstrução do esôfago (3,20%. Dezesseis (16 animais permaneceram sem diagnóstico, dos quais nove (28,48% apresentavam avançado estado autolítico e em sete (21,60% não foram observadas lesões macro e microscópicas compatíveis com nenhuma enfermidade. Este artigo apresenta relatos de doenças ainda não descritas em cutias e seus resultados poderão produzir literatura sobre os aspectos patológicos destas enfermidades nessa espécie.Necroscopic examination is remarkably useful to diagnose wild animal's diseases. In several occasions the clinical signs are not charactheristic as in domestic animals and the occurrence of death in asymptomatic animals is frequent. Thus, the present work aimed to accomplish a retrospective study on agouti diseases diagnosed by pathological examination in the Laboratory of Veterinary Pathology, Federal Rural University of the Semi-arid, through January 2006 to December 2006. In 32 agoutis submitted to the necropsy, the percentage of diagnosed diseases was: perinatal death due hypothermia/ inanition complex (21.6%, obstructive urolithiasis (6.24%, dystocia (6.24%, cecum sablosis (6.24%, intussusceptions (3.20%, fecaloma (3.20% and esophagus obstruction (3.20%. A total of 16 (50.08% animals remained undiagnosed in which nine (28.48% showed advanced autolysis and seven (21.60% agouti had none macroscopic or microscopic lesions compatible with any disease. The present article presents reports of some diseases not yet diagnosed in agoutis and these results may produce literature review about the pathologic aspects of these diseases in this species.

  6. PERFIL METABÓLICO DE OVELHAS SANTA INÊS EM DIFERENTES FASES DE GESTAÇÃO CRIADAS EM SISTEMA SEMI-INTENSIVO NO ESTADO DO AMAZONAS

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    Roseane Pinto Martins de Oliveira

    2014-03-01

    Full Text Available The aim of this study was to evaluate the nutritional metabolic condition of ewes at different stages of pregnancy. The analysis of the metabolic profile was through blood collection, using 30 sheep divided into three groups according to the stage of gestation (days: G1: between 50 and 60 days; G2: between 80 and 90 days, and G3: between 130 and 140 days. The analyzes of metabolites were performed by commercial kits. The concentrations of total protein, albumin, globulin and urea did not differ, except for urea, which showed increased levels. The mean glucose remained below the normal minimum value. For cholesterol, the means were 89.86 mg / dL, 86.01 mg / dL and 92.23 mg / dL for G1, G2 and G3, respectively. The animals of G1 and G2 presented statistically similar means for triglycerides, differing from G3 animals. The β-hydroxybutyrate mean remained within the reference. Calcium levels were below the reference, showing the lowest level in G3 (8.86 mg/dL. Phosphorus levels decreased in G3. Any stage of pregnancy showed severe deficiencies in protein energy intake and diet, however requiring adequate calcium and phosphorus to the end of pregnancy.

  7. Análise de sobrevivência e estimativa de entropia para Sarconesia chlorogaster (Wiedemann (Diptera, Calliphoridae Survival analysis and estimation of entropy of Sarconesia chlorogaster (Wiedemann (Diptera, Calliphoridae

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    Maurício O. Moura

    1999-01-01

    Full Text Available The life expectancy of Sarconesia chlorogaster (Wiedemann, 1830 reared in artificial diet and controlled temperatures chamber set at 27±1oC, 70±10% RH and 12 hours of photophase was analysed using entropy (H. Entropy (H was used to quantify the distribuition of deaths between ages and then quantify the impact of mortality on life expectancy. The entropy values obtained for males (H=0,245 and females (H=0,299 were intermediary between the theoretical values of H=0,5 and H=0 suggesting a tendency toward rectangular distribuitions in both sexes. The effect of mortality across ali ages on expectation of life was different at each age. For males the highest values were found between days 10 and 20 and between days 15 and 25 for females. This íindings imply that small changes in female mortality will have a greater impact on female life expectancy than will have on male expectation of life.

  8. Dietas naturais na criação de Chrysomya albiceps (Wiedemann, 1819; Diptera: Calliphoridae): estudo comparado Natural diets to rear Chrysomya albiceps (Wiedemann, 1819; Diptera: Calliphoridae): a comparative study

    OpenAIRE

    Rosemary Cardoso Ribeiro; Eliane Maria Vieira Milward-de-Azevedo

    1997-01-01

    Procurou-se observar o desenvolvimento pós-embrionário de Chrysomya albiceps, testando-se, comparativamente, dietas à base de sardinha e carne eqüina. Os substratos frescos foram previamente mantidos a 30°C de temperatura durante duas horas. Foram inoculadas 50 neolarvas em 100g de dieta por repetição. Utilizaram-se quatro repetições por tratamento. O experimento foi conduzido em condições de laboratório. O peso médio das larvas após o abandono espontâneo das dietas foi de 91,19mg, no sub...

  9. Dietas naturais na criação de Chrysomya albiceps (Wiedemann, 1819; Diptera: Calliphoridae: estudo comparado Natural diets to rear Chrysomya albiceps (Wiedemann, 1819; Diptera: Calliphoridae: a comparative study

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    Rosemary Cardoso Ribeiro

    1997-12-01

    Full Text Available Procurou-se observar o desenvolvimento pós-embrionário de Chrysomya albiceps, testando-se, comparativamente, dietas à base de sardinha e carne eqüina. Os substratos frescos foram previamente mantidos a 30°C de temperatura durante duas horas. Foram inoculadas 50 neolarvas em 100g de dieta por repetição. Utilizaram-se quatro repetições por tratamento. O experimento foi conduzido em condições de laboratório. O peso médio das larvas após o abandono espontâneo das dietas foi de 91,19mg, no substrato à base de carne eqüina e 76,01mg, no substrato à base de sardinha, a diferença foi, portanto, significativa. A taxa de sobrevivência larval registrada foi superior a 80%, enquanto a taxa de sobrevivência pupal foi próxima a 100%. A razão sexual foi próxima a 0,5. A dieta à base de carne eqüina mostrou-se mais adequada ao desenvolvimento de C. albiceps, pois as larvas mantidas neste substrato apresentaram-se mais pesadas, o que potencializa a capacidade reprodutiva do adulto.The objective of the present study was to observe the postembryonic development of Chrysomya albiceps reared on sardines or horse meat and to compare the results. Fresh substratos were maintened m an oven ai 30°C for 2 hours. Fifty newly emerged larvas were inoculated into 100g diel per reptication. Four replications per treatment were used. The experiment was conducted under laboratory conditions. The mean weight of postfeeding larvas was 91.19mg for especimens reared on horse meat and 76.01mg for especimens reared on sardines, with a marked difference between diets. Larval viability was more than 80% for the two diets, while pupal viability was dose to 100%. The sex ratio was about 0.5. The horse meat diet proved to be more adequate for the development of C. albiceps since the larvas weighed more, with consequent potentiations of adult reproductive abilily.

  10. Morfologia e duração dos ínstares larvais de Chrysomya putoria (Wiedemann (Diptera, Calliphoridae, em laboratório Morphology and duration of larval instars of Chrysomya putoria (Wiedemann (Diptera, Calliphoridae, in laboratory

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    Marcio Sacramento de Oliveira

    2007-01-01

    Full Text Available Este trabalho visou descrever a morfologia e o tempo de desenvolvimento das larvas de Chrysomya putoria, um importante díptero para saúde pública e medicina legal. As amostras foram coletadas a cada duas horas e, a partir da 56ª hora, as coletas foram realizadas com intervalos de 12 horas até o abandono das larvas maduras do meio de criação. As larvas de C. putoria apresentaram um tempo total de desenvolvimento de 92 horas quando mantidas a 27 ºC com comprimento médio de 13,42 mm e características morfológicas peculiares que as diferenciam das outras espécies exóticas e autóctones do Brasil. O esqueleto cefalofaringeano das larvas de terceiro ínstar é robusto e esclerotizado; o gancho labial é completamente pigmentado e curvo, a parte posterior é mais larga que a anterior, mostrando-se levemente pontiagudo. Estas larvas não possuem esclerito oral acessório e o corno dorsal e o ventral apresentam áreas menos pigmentadas. Nota-se uma região não pigmentada em continuação aos cornos, destacando-se no tamanho na região do corno ventral com uma incisão média bem demarcada. Apresentam de 10 a 11 ramificações no espiráculo anterior, peritrema incompleto e presença do botão espiracular, a partir do segundo ínstar.The objective of this work was to describe the morphology and the developmental time of Chrysomya putoria larvae, a fly of public health and forensic importance. The samples were collected at 2 hr intervals, during the first 56 hr and at 12 hr intervals thereafter, until all mature larvae had abandoned the diet. The total developmental time for Chrysomya putoria larvae at 27 ºC was 92 hr, with a mean length of 13.42 mm. The larvae presented unique morphological characteristics which differentiated them from other exotic and autochthonous species found in Brazil. The cephalopharyngeal skeleton of the third instar larvae is robust and sclerotized; the mouth hook is completely pigmented and curved; the posterior end is wider than the anterior, being slightly pointed. The accessory oral sclerit is absent and the dorsal and ventral horns have areas with less pigmentation. It is possible to visualize an area with no pigmentation adjacent to the horns, outstanding in size near the ventral corn with a well delimited medial incision. The anterior spiracles presented 10 to 11 ramifications, open peritrema and the presence of a spiracular bud from the second instar on.

  11. Study of the combined radial post-feeding dispersion of the blowflies Chrysomya megacephala (Fabricius and C. albiceps (Wiedemann (Diptera, Calliphoridae Estudo da dispersão radial combinada de Chrysomya megacephala (Fabricius e C. albiceps (Wiedemann (Diptera, Calliphoridae

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    Leonardo Gomes

    2005-09-01

    Full Text Available Blowflies use discrete and ephemeral substrates to feed their larvae. After they run out of food, the larvae begin to disperse in order to find adequate places for pupation or additional food sources, a process named post-feeding larval dispersion. Some important aspects of this process were studied in a circular arena allowing the combined radial post-feeding dispersion from the center of the arena of C. albiceps and C. megacephala larvae. To determine the location of each pupa, the arena was divided in 72 identical sections starting from the center. The distance from the center, the depth and weight of each pupa were evaluated. Statistical tests were done to verify the relation between weight, depth and distance for pupation. From the total an average of 976 larvae released (488 for each species were collected considering both experiments 456 C. megacephala pupae and 488 of C. albiceps. This demonstrates that C. albiceps probably preyed on 32 C. megacephala larvae during post-feeding dispersion. The study of this dispersion process can be used to estimate the postmortem interval (PMI of human cadavers in legal medicine.As moscas- varejeiras utilizam-se de substratos discretos e efêmeros para alimentar suas larvas. Após deixarem o substrato alimentar, as larvas começam a dispersar em busca de locais adequados para pupação e fontes adicionais de alimento, um processo denominado dispersão larval pós-alimentar. Alguns aspectos importantes desse processo foram estudados em uma arena permitindo a dispersão radial combinada de larvas de C. megacephala e C. albiceps. Para determinar a localização de cada pupa, a arena foi dividida em 72 setores iguais começando do centro. A distância a partir do centro, a profundidade e o peso de cada pupa foram determinados. Testes estatísticos foram feitos para verificar a relação entre peso, profundidade e distância para pupação. De um total em média de 976 larvas soltas (488 de cada espécie foram coletadas considerando ambos os experimentos 456 larvas de C. megacephala e 488 larvas de C. albiceps. Isso demonstrou que as larvas de C. albiceps provavelmente predaram 32 larvas de C. megacephala. O estudo desse processo de dispersão pode auxiliar na estimativa do intervalo pós- morte (IPM em estudos de medicina legal.

  12. Dispersão larval pós-alimentar de Lucilia sericata (Diptera, Calliphoridae em condições de laboratório Post-feeding larval dispersion of Lucilia sericata (Diptera, Calliphoridae in laboratory

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    Sabrina M Pires

    2010-09-01

    Full Text Available Os dípteros califorídeos são os primeiros indivíduos a compor a sucessão faunística de cadáveres em decomposição, auxiliando assim na estimativa do intervalo pós-morte. Estudos de dispersão pós-alimentar de suas larvas possuem relevância para investigações médico-criminais. Diante disto, uma arena circular, simulando o ambiente natural, foi montada em laboratório a fim de verificar-se a dispersão larval radial pós-alimentar de Lucilia sericata (Meigen, 1826, tentando assim estabelecer relações entre as variáveis peso, distância e profundidade de enterramento na arena. Os resultados demonstraram que 45% das pupas foram recuperadas a uma profundidade compreendida entre 6 cm e 8 cm e a uma distância entre 33 cm e 45 cm do centro da arena. Não houve diferenças significativas quanto à propagação e profundidade de enterramento de machos, fêmeas e indivíduos inviáveis. Com relação ao peso, verificou-se que a média das fêmeas (x = 32,35 mg foi superior a dos machos (x = 30,28 mg. A análise de correlação e de regressão entre peso e distância percorrida e entre peso e profundidade foram positivas, ou seja, pupas oriundas de larvas mais pesadas propagaram e se aprofundaram mais. O experimento permitiu ainda concluir que uma arena circular possibilita o deslocamento das larvas em todas as direções.Calliphorid flies are the first organisms of the faunal succession associated with decomposing bodies, and can help in the estimation of post-mortem interval. Therefore, studies on post-feeding larval dispersion are relevant to medico-criminal investigations. A circular arena simulating the natural environment was built in the laboratory in order to examine the radial post-feeding larval dispersion of Lucilia sericata (Meigen, 1826 and the relationship between weight, distance, and depth of burial. Our findings indicated that most pupae were recovered at a depth between 6 cm and 8 cm and at a distance of 33 cm and 45 cm from the center of the arena. No significant differences were found regarding the propagation and depth of males, females, and non-viable individuals. The mean weight of females (x = 32.35 mg was higher than that of males (x = 30.28 mg. The correlation and regression analysis between weight and distance, and between weight and depth was directly proportional suggesting that heavier pupae dispersed farther and deeper. Our study also showed that a circular arena allows the dispersion of larvae into all directions.

  13. Identification of forensically important blow fly species (Diptera: Calliphoridae) in China by mitochondrial cytochrome oxidase I gene differentiation

    Institute of Scientific and Technical Information of China (English)

    Qin-Lai Liu; Li Yang; Kun-Lu Wu; Ling-mei Lan; Jiang-Feng Wang; Yao-Qing Chen; Ji-Feng Cai; Yun-Feng Chang; Yan Gu; Ya-Dong Guo; Xing-Hua Wang; Ji-FangWeng; Ming Zhong; Xiang Wang

    2011-01-01

    Unambiguous and rapid sarcosaphagous insect species identification is an essential requirement for forensic investigations. Although some insect species are difficult to classify morphologically, they can be effectively identified using molecular methods based on similarity with abundant authenticated reference DNA sequences in local databases.However, local databases are still relatively incomplete in China because of the large land area with distinct regional conditions. In this study, 75 forensically important blow flies were collected from 23 locations in 16 Chinese provinces, and a 278-bp segment of the cytochrome oxidase subunit Ⅰ gene of all specimens was successfully sequenced. Phylogenetic analysis of the sequenced segments showed that all Calliphorid specimens were properly assigned into nine species with relatively strong supporting values, thus indicating that the 278-bp cytochrome oxidase subunit one region is suitable for identification of Calliphorid species. The clear difference between intraspecific threshold and interspecific divergence confirmed the potential of this region for Calliphorid species identification,especially for distinguishing between morphologically similar species. Intraspecific geographic variations were observed in Lucilia sericata (Meigen, 1826) and Lucilia caesar (Linnaeus, 1758).

  14. Miíase por Lucilia eximia (Diptera: Calliphoridae em Didelphis albiventris (Mammalia: Didelphidae no Brasil Central

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    Edison Cansi

    2011-12-01

    Abstract. In May 2009 were collected 18 larvae of Lucilia eximia (Wiedemann, a fly responsible for primary and secondary myiasis in livestock and humans. The larvae were taken from the myiasis on anal and auricular regions of an opossum Didelphis albiventris (Lund, in Brasília Zoo, and later identified in the laboratory. After 15 days, 15 adults emerged from L. eximia. This is the first record of this blowfly causing a primary myiasis in a marsupial species in the Brasília Cerrado.

  15. Meat Feeding Restricts Rapid Cold Hardening Response and Increases Thermal Activity Thresholds of Adult Blow Flies, Calliphora vicina (Diptera: Calliphoridae).

    Science.gov (United States)

    Coleman, Paul C; Bale, Jeffrey S; Hayward, Scott A L

    2015-01-01

    Virtually all temperate insects survive the winter by entering a physiological state of reduced metabolic activity termed diapause. However, there is increasing evidence that climate change is disrupting the diapause response resulting in non-diapause life stages encountering periods of winter cold. This is a significant problem for adult life stages in particular, as they must remain mobile, periodically feed, and potentially initiate reproductive development at a time when resources should be diverted to enhance stress tolerance. Here we present the first evidence of protein/meat feeding restricting rapid cold hardening (RCH) ability and increasing low temperature activity thresholds. No RCH response was noted in adult female blow flies (Calliphora vicina Robineau-Desvoidy) fed a sugar, water and liver (SWL) diet, while a strong RCH response was seen in females fed a diet of sugar and water (SW) only. The RCH response in SW flies was induced at temperatures as high as 10°C, but was strongest following 3h at 0°C. The CTmin (loss of coordinated movement) and chill coma (final appendage twitch) temperature of SWL females (-0.3 ± 0.5°C and -4.9 ± 0.5°C, respectively) was significantly higher than for SW females (-3.2 ± 0.8°C and -8.5 ± 0.6°C). We confirmed this was not directly the result of altered extracellular K+, as activity thresholds of alanine-fed adults were not significantly different from SW flies. Instead we suggest the loss of cold tolerance is more likely the result of diverting resource allocation to egg development. Between 2009 and 2013 winter air temperatures in Birmingham, UK, fell below the CTmin of SW and SWL flies on 63 and 195 days, respectively, suggesting differential exposure to chill injury depending on whether adults had access to meat or not. We conclude that disruption of diapause could significantly impact on winter survival through loss of synchrony in the timing of active feeding and reproductive development with favourable temperature conditions.

  16. Meat Feeding Restricts Rapid Cold Hardening Response and Increases Thermal Activity Thresholds of Adult Blow Flies, Calliphora vicina (Diptera: Calliphoridae.

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    Paul C Coleman

    Full Text Available Virtually all temperate insects survive the winter by entering a physiological state of reduced metabolic activity termed diapause. However, there is increasing evidence that climate change is disrupting the diapause response resulting in non-diapause life stages encountering periods of winter cold. This is a significant problem for adult life stages in particular, as they must remain mobile, periodically feed, and potentially initiate reproductive development at a time when resources should be diverted to enhance stress tolerance. Here we present the first evidence of protein/meat feeding restricting rapid cold hardening (RCH ability and increasing low temperature activity thresholds. No RCH response was noted in adult female blow flies (Calliphora vicina Robineau-Desvoidy fed a sugar, water and liver (SWL diet, while a strong RCH response was seen in females fed a diet of sugar and water (SW only. The RCH response in SW flies was induced at temperatures as high as 10°C, but was strongest following 3h at 0°C. The CTmin (loss of coordinated movement and chill coma (final appendage twitch temperature of SWL females (-0.3 ± 0.5°C and -4.9 ± 0.5°C, respectively was significantly higher than for SW females (-3.2 ± 0.8°C and -8.5 ± 0.6°C. We confirmed this was not directly the result of altered extracellular K+, as activity thresholds of alanine-fed adults were not significantly different from SW flies. Instead we suggest the loss of cold tolerance is more likely the result of diverting resource allocation to egg development. Between 2009 and 2013 winter air temperatures in Birmingham, UK, fell below the CTmin of SW and SWL flies on 63 and 195 days, respectively, suggesting differential exposure to chill injury depending on whether adults had access to meat or not. We conclude that disruption of diapause could significantly impact on winter survival through loss of synchrony in the timing of active feeding and reproductive development with favourable temperature conditions.

  17. Spatio-temporal dynamics and transition from asymptotic equilibrium to bounded oscillations in Chrysomya albiceps (Diptera, Calliphoridae

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    Wesley Augusto Conde Godoy

    2001-07-01

    Full Text Available The sensitivity of parameters that govern the stability of population size in Chrysomya albiceps and describe its spatial dynamics was evaluated in this study. The dynamics was modeled using a density-dependent model of population growth. Our simulations show that variation in fecundity and mainly in survival has marked effect on the dynamics and indicates the possibility of transitions from one-point equilibrium to bounded oscillations. C. albiceps exhibits a two-point limit cycle, but the introduction of diffusive dispersal induces an evident qualitative shift from two-point limit cycle to a one fixed-point dynamics. Population dynamics of C. albiceps is here compared to dynamics of Cochliomyia macellaria, C. megacephala and C. putoria.

  18. Seasonal variations in bacterial communities and antibiotic-resistant strains associated with green bottle flies (Diptera: Calliphoridae).

    Science.gov (United States)

    Wei, Ting; Ishida, Ryuichi; Miyanaga, Kazuhiko; Tanji, Yasunori

    2014-05-01

    Green bottle flies occur frequently around human environments in Japan. Many species of green bottle flies have been studied with regard to their importance in forensic examinations or clinical therapies, but the bacterial communities associated with this group of flies have not been comprehensively investigated. In this research, 454 pyrosequencing was used to reveal the bacterial communities in green bottle flies collected in different seasons. Meanwhile, the bacteria were screened with selective media and tested for antibiotic susceptibility. Samples collected in three different seasons harbored distinctive bacterial communities. The predominant genera associated with green bottles flies were Staphylococcus in spring, Ignatzschineria in summer, and Vagococcus, Dysgonomonas, and an unclassified Acetobacteraceae in autumn. An upward trend in bacterial community diversity was observed from spring to autumn. Changes in climatic conditions could be the cause of these seasonal variations in fly-associated bacterial communities. The species of isolated antibiotic-resistant bacteria also differed across seasons, but it was difficult to correlate seasonal changes in antibiotic-resistant bacteria with changes in whole communities. A number of multiple-antibiotic-resistant bacteria were isolated, and some of these strains were closely affiliated with pathogens such as Enterococcus faecalis and Enterococcus faecium, which could cause serious threats to public health. Overall, this research provided us with information about the composition and seasonality of bacterial communities in green bottle flies, and highlighted the risks of fly-mediated dissemination of antibiotic-resistant pathogens.

  19. Mesembrinellinae (Diptera: Calliphoridae to edge effects in the Tinguá Biological Reserve, Rio de Janeiro, Brazil

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    B. Q. Gadelha

    Full Text Available Abstract In this study we describe the diversity of Mesembrinelinae in a biological reserve in the city of Nova Iguaçu, State of Rio de Janeiro. Traps containing sardines were distributed seasonally, at four sites: Site A (22° 58.788’ S, 43° 43.459’ W, in a forest edge, and sites B (22° 58.523’ S, 43° 44.540’ W, C (22° 58.350’ S, 43° 44.678’ W, and D (22° 34.865’ S, 43° 27.063’ W, located 1,000m, 500m, and 2,000m respectively, inwards from the edge. A total of 2,150 individuals of Mesembrinellinae were collected, representing ten species. Laneela nigripes Guimarães, 1977 was the most abundant species, followed by Mesembrinella bellardiana Aldrich, 1922, Eumesembrinella cyaneicincta (Surcouf, 1919 and Mesembrinella semihyalina Mello, 1967. These species were common and constant during the study period. Mesembrinella batesi Aldrich, 1922, Eumesembrinella quadrilineata (Fabricius, 1805 and Huascaromusca aeneiventris (Wiedmann, 1830 were the less abundant flies, being considered rare and accidental. Eumesembrinella besnoiti (Seguy, 1925 was rare and accessory. Eumesembrinella cyaneicincta, M. bellardiana, M. semihyalina and M. bicolor were mostly collected in site B, while L. nigripes was mostly collected in site C. The edge effect was not evident since the four sites showed similar populations. Site B showed a strong positive relationship between abundance and richness, in site C the correlation was positive and weak, and there was no correlation in A and D. The highest abundance of specimens was recorded during autumn and winter. These flies occurred from the edge up to 2,000m inside the forest.

  20. Caracterización citogenética de lucilia sericata (Meigen, 1826 (Diptera: calliphoridae, Cepa Bogotá, Colombia

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    Yisethe Acuña Morera

    2011-08-01

    Full Text Available Objective: Lucilia sericata is an important species for medical and forensic purposes, it is used in maggot therapy in the treatment of chronic wounds and in medical-legal studies for establishing the post-mortem interval and the transfer of corpses. Currently there are no records of the cytogenetic characteristics of this fly in Neotropical region. The main objective of this study wasto identify morphometric characteristics and primary structures fromkaryotype of L. sericata strain Bogota, Colombia. Methods and materials: Embryonated eggs were taken, which were previously surface sterilized, macerated and then seeded in L-15 medium culture, supplemented with 20% fbs and incubated at 28 ºC, without C02 atmosphere. The preparation of chromosomeswas obtained from semiconfluent monolayers, pretreated with various solutions: antimitotic (Colchicine, hypotonic (KCl 0.075 M and fixative (Carnoy, methanol and acetic acid, 3:1. C-banding technique was carried out to identify chromosomal regions of constitutive heterochromatin. Results: Morphometric parameters were obtained from each pair of chromosomes. The diploid karyotype number obtained from cell cultures was 2n = 12; they were classified morphologically,according to patterns established previously, as follows: pairs I,ç II, IV and V were metacentric and pair III was submetacentric. On the other hand, the sexual pair was heteromorphic, being X chromosome metacentric and Y chromosome submetacentric. C banding was positive for all chromosome pairs. Conclusions: The cytogenetic characteristics of L. sericata, strain Bogotá, were established according to number, shape, centromer position and heterochromatic regions.

  1. Estudo da dispersão larval radial pós-alimentar em Chrysomya megacephala (Fabricius (Diptera, Calliphoridae

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    Gomes Leonardo

    2003-01-01

    Full Text Available Blowflies utilize discrete and ephemeral sites for breeding and larval nutrition. After the exhaustion of food, the larvae begin dispersing to search for sites to pupate or to additional food source, process referred as postfeeding larval dispersal. Some of the most important aspects of this process were investigated in Chrysomya megacephala, utilizing a circular arena to permit the radial dispersion of larvae from the center. To determinate the localization of each pupa, the arena was split in 72 equal sectors from the center. For each pupa, distance from the center of arena, weight and depth were determined. Statistical tests were performed to verify the relation among weight, depth and distance of burying for pupation. It was verified that the larvae that disperse farther are those with higher weights. The majority of individuals reached the depth of burying for pupation between 7 and 18 cm. The study of this process of dispersion can be utilized in the estimation of postmortem interval (PMI for human corpses in medico-criminal investigations.

  2. Calliphora vicina (Diptera: Calliphoridae) pupae: a timeline of external morphological development and a new age and PMI estimation tool.

    Science.gov (United States)

    Brown, Katherine; Thorne, Alan; Harvey, Michelle

    2015-07-01

    The minimum postmortem interval (PMI(min)) is commonly estimated using calliphorid larvae, for which there are established age estimation methods based on morphological and development data. Despite the increased duration and sedentary nature of the pupal stage of the blowfly, morphological age estimation methods are poorly documented and infrequently used for PMI determination. The aim of this study was to develop a timeline of metamorphosis, focusing on the development of external morphology (within the puparium), to provide a means of age and PMI estimation for Calliphora vicina (Rob-Desvoidy) pupae. Under controlled conditions, 1,494 pupae were reared and sampled at regular time intervals. After puparium removal, observations of 23 external metamorphic developments were correlated to age in accumulated degree hours (ADH). Two age estimation methods were developed based on (1) the combination of possible age ranges observed for each characteristic and (2) regression analyses to generate age estimation equations employing all 23 characteristics observed and a subset of ten characteristics most significantly correlated with age. Blind sample analysis indicated that, using the combination of both methods, pupal age could be estimated to within ±500 ADH with 95% reliability.

  3. Determinación de la actividad antibacteriana de los extractos y hemolinfa de larvas de Lucilia eximia (Diptera: Calliphoridae)

    OpenAIRE

    Giraldo Hincapié, Paula Andrea

    2014-01-01

    Resumen: Lucilia eximia es una especie neotropical, de importancia médica y forense que ha sido utilizada en la terapia larval como método de desinfección, desbridamiento y cicatrización de heridas ulcerativas en pacientes en la ciudad de Medellín. En el presente trabajo, mediante técnicas de ultrafiltración y cromatografía de alta resolución (HPLC) se realizó una búsqueda preliminar de compuestos con actividad antibacteriana, presentes en exosecreciones y hemolinfa de larvas de Lucilia e...

  4. Evaluating the Effects of Different Vegetation Types on Necrophagous Fly Communities (Diptera: Calliphoridae; Sarcophagidae): Implications for Conservation

    Science.gov (United States)

    Pereira de Sousa, José Roberto; Carvalho-Filho, Fernando da Silva; Juen, Leandro; Esposito, Maria Cristina

    2016-01-01

    The present study was conducted in five different phytogeographic zones of the Brazilian state of Maranhão, three of which (the Amazon Forest, Cerrado, and Palm Groves) are more heterogeneous, whereas the other two (Marshlands and Mangroves) are more homogeneous. In each zone, nine sites were visited for the collection of necrophagous flies using bait traps in 2010, 2011, and 2012. The calliphorid and sarcophagid communities observed at each site were compared in terms of species richness, composition, and abundance. The more heterogeneous zones had higher species richness, except in the case of the sarcophagids in the forest habitats. The calliphorids Chloroprocta idioidea (Robineau- Desvoidy, 1830), Mesembrinella bicolor (Fabricius, 1805), Hemilucilia semidiaphana (Rondani, 1850) and Lucilia eximia (Wiedemann, 1819) were more closely associated with the Cerrado, Palm Grove and Amazon Forest zones, and Chrysomya megacephala (Fabricius, 194) with the Mangrove. In the sarcophagids, Peckia (Euboettcheria) subducta (Lopes, 1935) and P. (Pattonella) palidipilosa (Curran & Walley, 1934) were associated with the Amazon Forest, and P. (Sarcodexia) lambens (Wiedemann, 1830) and Tricharaea (Sarcophagula) occidua (Fabricius, 1794) with the Palm Grove and Cerrado zones. In the calliphorids, the greatest dissimilarity was recorded between the Amazon Forest and the Mangrove and Lowland grassland zones. In the sarcophagids, by contrast, the greatest dissimilarities were recorded between the Amazon Forest and all the other four zones. In general, then, the phytogeographic zones with the highest environmental heterogeneity were characterized by the greatest species richness and abundance of necrophagous flies. PMID:27798664

  5. Estimating the age of Calliphora vicina eggs (Diptera: Calliphoridae): determination of embryonic morphological landmarks and preservation of egg samples.

    Science.gov (United States)

    Martín-Vega, Daniel; Hall, Martin J R

    2016-05-01

    Blow fly eggs may sometimes be the only entomological evidence recovered in a forensic case, especially in cooler weather when hatching might take several days: hence, a method for estimating their age is greatly needed. However, developmental data on blow fly eggs are mainly limited to records of the time to larval hatching. The current paper describes the morphological changes occurring during embryogenesis of the blow fly Calliphora vicina Robineau-Desvoidy and their timing in relation to temperature, in order to determine those characters which can be used for simple egg age estimation using light microscopy. At 7.3 and 25 °C, 15 easily visualised morphological landmarks were determined in C. vicina living embryos, allowing for their age estimation with a resolution of 10-20% of total egg developmental time. The observed age intervals were compared to the embryonic stages described for the fruit fly Drosophila melanogaster Meigen, which are used as reference data in multiple developmental studies. Moreover, current guidelines for preservation of egg samples, which recommend the placement of living eggs directly into 80% ethanol, were tested against the hot water killing (HWK) method prior to preservation in 80% ethanol, recommended for larval and pupal specimens. Direct placement of eggs into 80% ethanol caused marked decomposition of samples, and no morphological landmarks were discernible. On the other hand, HWK fixation prior to preservation in 80% ethanol enabled visualisation of 11 of the 15 age-specific morphological landmarks that were discernible in living embryos. Therefore, HWK fixation prior to preservation in 80% ethanol is recommended for egg samples, thus unifying the protocols for collecting entomological evidence.

  6. Study of some biological aspects of the blowfly Chrysomya albiceps (Wiedemann 1819 (Diptera: Calliphoridae in Jeddah, Saudi Arabia

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    Layla A.H. Al-Shareef

    2016-03-01

    Full Text Available We reared Chrysomya albiceps (Wiedemann 1819 unadult stages (first larval instar, second larval instar, third larval instar and pupal stage under four constant temperatures. Results proved that increasing temperature from 20 to 25, 30 and 35 °C reduced total larval stage duration (9–6, 4.83 and 4.75 days, respectively and pupal duration (7, 5.5, 4 and 1.5 days, respectively. C. albiceps larvae at first instar reached adult stage in the longest time at 20 °C (16 days, and in the shortest time at 35 °C (6.25 days. The accumulation degree-day (ADD at 20, 25, 30, 35 °C for first larval instar were 8.86, 13.86, 18.86, 23.86 DD, for second larval instar were 10.5, 12, 17, 22 DD and for third larval instar were 35.88, 42.08, 43.97, 56.43 DD. Heat requirements for larval stage at different temperatures; 20, 25, 30 and 35 °C (49.68, 63.12, 75.01 and 97.47 DD were more than the pupal requirements at the same temperatures (39.78, 58.76, 62.73 and 31.02 DD. Total heat requirements for C. albiceps to develop from the first larval instar to adult eclosion were the lowest at 20 °C (89.46 DD and the highest at 30 °C (129.138 DD. Decreasing of temperature increased larval body length at the same age. The development curves for C. albiceps were established at four constant temperatures using larval length and the time since egg hatching.

  7. Lucilia silvarum Meigen, 1826 (Diptera: Calliphoridae)--a new species of interest for forensic entomology in Europe.

    Science.gov (United States)

    Fremdt, Heike; Szpila, Krzysztof; Huijbregts, Johannes; Lindström, Anders; Zehner, Richard; Amendt, Jens

    2012-10-10

    In Europe, the blowfly genus Lucilia is represented in Forensic Entomology mainly by the species L. ampullacea, L. caesar, L. illustris and L. sericata. In the US, Lucilia silvarum is rarely recorded as a carrion breeding species but usually as a more or less exclusive parasite of frogs and toads. We present three forensic cases from different European countries reporting, for the first time, L. silvarum on human bodies that were found close to lakes, wetlands, or riversides. To use this species for post-mortem interval estimations, thermal development data is needed. The first step is accurate identification by morphological and molecular means. Therefore, we analysed a 611 bp part of the mitochondrial COI region for 23 specimens of L. silvarum from 9 different geographical regions, all of which give the same haplotype. Differences within the haplotype varied by up to 0.2%. Comparison between the haplotype found and those published on GenBank showed up to 1.2% variance. Moreover, we present an updated key for the morphological identification of the third larval instars of European Lucilia spp. of forensic importance, adding not only L. silvarum, but also L. cuprina which was recorded in Europe for the first time about 20 years ago.

  8. Do necrophagous blowflies (Diptera: Calliphoridae) lay their eggs in wounds?: Experimental data and implications for forensic entomology.

    Science.gov (United States)

    Charabidze, Damien; Depeme, Aurore; Devigne, Cedric; Hedouin, Valery

    2015-08-01

    This study was designed to examine the common belief that necrophagous blowflies lay their eggs in wounds. The egg-laying behaviour of Lucilia sericata was observed under controlled conditions on wet, artificially wounded or short-haired areas of rat cadavers. Flies laid significantly more eggs on the wet area and the area with short hair than on the dry area or area with long hair. No eggs were observed inside the wounds in any of the replicates. The effect of egg immersion (body fluids often exudes in wounds) on the survival rate of larvae was also investigated. In low water condition, an average of 72.7±7.9% of the larvae survived and they reached a mean length of 7.5±0.6mm. In contrast, submerging eggs under a high volume of water strongly affected their survival rate (25±3.7%) and development. Similar results were observed using unfrozen pig blood instead of water. These data question the information found in the literature regarding the preferential egg-laying behaviour of Calliphorids flies in wounds.

  9. Preliminary screening of the larvicidal effect of Brevibacillus laterosporus strains against the blowfly Chrysomya megacephala(Fabricius, 1794 (Diptera: Calliphoridae

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    Isabel Nogueira Carramaschi

    2015-08-01

    Full Text Available AbstractINTRODUCTION: This study evaluated whether different strains of Brevibacillus laterosporus could be used to control larvae of the blowfly Chrysomya megacephala , a pest that affects both human and animal health.METHODS:Mortality rates were recorded after 1-mL suspensions of sporulated cells of 14 different strains of B. laterosporus were added to 2.5g of premixed diet consisting of rotting ground beef fed to first instar larvae of C. megacephala . All bioassays were performed using 10 larvae per strain, with a minimum of three replicates for each bioassay. Larval mortality was recorded daily up to seven days.RESULTS:Strains Bon 707, IGM 16-92, and Shi 3 showed the highest toxicity toward the larvae producing 70.5%, 64.5%, and 51.6% of larval mortality, respectively, which was significantly higher than that in the control group (p < 0.05. In contrast, strains NRS 1642, NRS 661, NRS 590 BL 856, NRS 342, ATCC 6457, Bon 712, and NRS 1247 showed limited or no pathogenic activity against the target larvae.CONCLUSIONS:Our preliminary data indicated that B. laterosporus could be used to develop bioinsecticides against C. megacephala .

  10. Preliminary screening of the larvicidal effect of Brevibacillus laterosporus strains against the blowfly Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae).

    Science.gov (United States)

    Carramaschi, Isabel Nogueira; Pereira, Lorrane de Andrade; Queiroz, Margareth Maria de Carvalho; Zahner, Viviane

    2015-01-01

    This study evaluated whether different strains of Brevibacillus laterosporus could be used to control larvae of the blowfly Chrysomya megacephala, a pest that affects both human and animal health. Mortality rates were recorded after 1-mL suspensions of sporulated cells of 14 different strains of B. laterosporus were added to 2.5g of premixed diet consisting of rotting ground beef fed to first instar larvae of C. megacephala. All bioassays were performed using 10 larvae per strain, with a minimum of three replicates for each bioassay. Larval mortality was recorded daily up to seven days. Strains Bon 707, IGM 16-92, and Shi 3 showed the highest toxicity toward the larvae producing 70.5%, 64.5%, and 51.6% of larval mortality, respectively, which was significantly higher than that in the control group (p bioinsecticides against C. megacephala.

  11. Estudo morfologico e histoquimico das glandulas sexuais acessorias de chrysomya putoria durante a ovogenese (wiedemann) (diptera : calliphoridae)

    OpenAIRE

    1995-01-01

    Resumo: Chrysomya putoria é uma mosca varejeira normalmente associada a locais com precárias condições de higiene. Na natureza ela cria-se em carcaças e no esterco que acumula-se sob as gaiolas de galinhas poedeiras. Esta mosca está no Brasil a cerca de duas décadas mas na Áftica, seu local de origem, ela é descrita como díptero causador facultativo de miíase. As glândulas sexuais acessórias de temeas tem um importante papel em diferentes etapas da fertilização do ovo e sua ausência experimen...

  12. The effect of blow flies (Diptera: Calliphoridae) on the size and weight of mangos (Mangifera indica L.)

    OpenAIRE

    Saeed, Shafqat; NAQQASH, Muhammad Nadir; Jaleel, Waqar; Saeed, Qamar; Ghouri, Fozia

    2016-01-01

    Background: Pollination has a great effect on the yield of fruit trees. Blow flies are considered as an effective pollinator compared to hand pollination in fruit orchards. Therefore, this study was designed to evaluate the effect of different pollination methods in mango orchards. Methodology: The impact of pollination on quantity and quality of mango yield by blow flies was estimated by using three treatments, i.e., open pollinated trees, trees were covered by a net in the presence of blow ...

  13. Influence of paraquat on Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) infesting minced-beef substrates in Kelantan, Malaysia.

    Science.gov (United States)

    Mahat, Naji A; Yin, Chu L; Jayaprakash, Paul T

    2014-03-01

    This study investigated the influence of paraquat, a prevalent poison used by suicides, on initial oviposition and development of Chrysomya megacephala (Fabricius) using minced-beef substrates. Paraquat in lethal dose for human (40 mg/kg), two times the lethal dose (80 mg/kg) and five times the lethal dose (200 mg/kg) were mixed thoroughly with respective minced-beef substrates (1 kg each) that were decomposed in a shaded habitat fully protected from rain. Results of four replications of the above experiment revealed that the presence of paraquat neither delayed initial oviposition nor prolonged the developmental stages of C. megacephala. Therefore, estimation of postmortem interval (PMI) based on empirical baseline data obtained using animal models devoid of any poisons would still be appropriate for estimating PMI in paraquat-related deaths.

  14. Influence of rain and malathion on the oviposition and development of blowflies (Diptera: Calliphoridae) infesting rabbit carcasses in Kelantan, Malaysia.

    Science.gov (United States)

    Mahat, N A; Zafarina, Z; Jayaprakash, P T

    2009-11-20

    The influence of rain and malathion on the initial oviposition as well as development of blowfly species infesting rabbit carcasses decomposing in sunlit and shaded habitats were studied over a period of 1 year in Kelantan, Malaysia. Chrysomya megacephala (Fabricius) was the most dominant species that infested the carcasses, followed by Chrysomya rufifacies (Macquart). In general, rain, depending on its intensity, delayed initial oviposition by 1-2 days and prolonged the pupation period by 1-3 days. The presence of malathion in the carcasses delayed initial oviposition by 1-3 days and prolonged the pupation period by 2-3 days. These findings deserve consideration while estimating postmortem interval since rain is a commonplace occurrence in Malaysia and malathion is one of the common poisons as an agent for choice to commit suicide.

  15. Biologia de Imaturos e Adultos de Podisus nigrispinus (Dallas (Hemiptera: Pentatomidae Alimentados com Lagartas de Dione juno juno (Cramer (Lepidoptera: Nymphalidae, criadas em Diferentes Genótipos de Maracujazeiro

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    Marina Angelini

    2015-12-01

    Abstract. The development of Podisus nigrispinus (Dallas (Hemiptera: Pentatomidae nymphs fed with Dione juno juno (Cramer (Lepidoptera: Nymphalidae larvae reared on leaves of the passion fruit genotypes Passiflora edulis Sims., Passiflora edulis f. flavicarpa Deg. (Seleção de Jaboticabal, P. edulis f. flavicarpa (cv. Sul Brasil and P. edulis f. flavicarpa (cv. Maguary FB-100, P. alata, P. serrato-digitata and P. foetida. The experiment was conducted under controlled conditions (temperature: 26 ± 1°C, RH = 60 ± 10% and photophase of 14 hours. Second instar nymphs of the predator were kept in plastic cups (4.5 cm high and 7.5 cm wide groups of five. Ten-day-old (approx. 2 cm D. juno juno larvae reared on leaves of different passion fruit genotypes were provided daily to P. nigrispinus. The experiment was carried out with 10 replications, totalizing 50 nymphs per treatment. Daily evaluations were performed to measure the duration and viability of each instar, the body mass of nymphs (24 hours after each ecdysis and adults, and the duration and viability of the nymph phase and adult longevity under starvation. Results show the influence of passion fruit genotypes at the third trophic level, since larvae reared with P. edulis f. flavicarpa (Seleção de Jaboticabal have shown to be more adequate for predator development. The results of this experiment show the influence of passion fruit genotypes on the third trophic level. The genotype P. edulis f. flavicarpa cv. Sul Brasil has a less appropriate to the predator, suggesting a negative way the association between host plant resistance and use of biological control. Already P. edulis and genotypes P. edulis f. flavicarpa Jaboticabal Selection can be used along with the predator because these cultivars did not affect the third trophic level. P. edulis f. flavicarpa cv. Maguary FB-100, considered ill-suited to the development of larvae of D. juno juno, affected the predator, resulting in a negative association between this genotype and biological control.

  16. Citologia do leite de búfalas (Bubalus bubalis hígidas criadas no Estado de São Paulo, Brasil Milk citology of healthy buffaloes (Bubalus bubalis bred in São Paulo state, Brazil

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    Alice Maria Melville Paiva Della Libera

    2004-08-01

    Full Text Available As avaliações quantitativas e qualitativas das células presentes no leite são empregadas para a compreensão de diversos processos, fisiológicos ou não da glândula mamária. Apesar disso, as citações da literatura são conflitantes quanto às búfalas sadias e, para elucidar essa questão, avaliou-se a celularidade do leite em 108 amostras de quartos mamários de búfalas hígidas em lactação, submetendo-as à contagem de células somáticas (CCS, automática e por microscopia óptica, além de estabelecer-se a predominância celular empregando duas técnicas (Prescott e Breed e citocentrifugação. Os resultados das CCS do leite, por técnicas microscópica e automática foram semelhantes, mas a predominância celular analisada por microscopia diferiu em valores relativos. Foram identificadas mais células mononucleares nas lâminas de suspensão celular citocentrifugada, e mais leucócitos polimorfonucleares na técnica de Prescott e Breed. A lâmina de citocentrifugação permitiu melhor avaliação da morfologia celular sendo identificados 61,1% de monócitos e macrófagos; 32,9% de neutrófilos; 5,3% de linfócitos e 0,7% de eosinófilos. Os fagócitos mononucleares apresentaram uma acentuada plasticidade de suas estruturas, com variados padrões morfológicos.The quantitative and qualitative evaluations of cells present on milk are important for understanding many events, physiologic or not, of the mammary gland. Despite that, citations in the literature are conflicting concerning healthy buffaloes. This study evaluated milk cellularity in 108 samples of healthy buffaloes mammary glands. They were analyzed by somatic cell count, automatic and microscopic, and the cellular types in slides of milk smear and also after cytocentrifugation of cellular suspension (differential citology evaluation. Results of milk somatic cells count, by microscopic and automatic techniques, were similar but cellular predominance (from Prescott - Breed or cytocentrifugation differed in relative values. More mononuclear cells were found in slides of cytocentrifugation of cellular suspension smears and more leucocytes on direct microscopic count technique. The cytocentrifugation smears showed a better evaluation of cellular morphology, identifying 61.1% of monocytes and macrophages, 32.9% of neutrophils, 5.3% of lymphocytes and 0.7% of eosinophils. Mononuclear phagocytes showed a high plasticity on structure with many morphological patterns.

  17. Qualidade do ovo de galinhas poedeiras criadas em galpões no semi-árido paraibano Quality of eggs of laying hens reared in poultry houses in the semi-arid Paraiba

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    Jair L. Trindade

    2007-12-01

    Full Text Available Avaliar índices ambientais dos galpões e os zootécnicos em aves de postura leve da linhagem Lohamann, em condições de verão e inverno de 2005 na região do semi-árida paraibana, foi o objetivo primordial deste trabalho, no qual se utilizaram 34.500 aves com diferentes idades: 32, 50, 60 e 71 semanas, acondicionadas em quatro galpões. Avaliaram-se os índices ambientais temperatura do ar, umidade relativa do ar, índice de temperatura do globo negro e umidade, carga térmica de radiação e velocidade do vento; e os produtivos (produção total de ovos, peso do ovo, peso do albúmen, peso da gema e peso das cascas, parâmetros geométricos (área e volume e a unidade Haugh (UH do ovo, em função da idade. Quanto ao ambiente no interior dos aviários, não houve diferença significativa na media dos índices ambientais, que ficaram dentro da zona de conforto. A idade das aves teve influência nos índices produção total, peso do ovo e peso da gema. A maior produção foi obtida com aves mais jovens, enquanto o peso do ovo e da gema foi com aves de maior idade. Os parâmetros geométricos volume e área do ovo não foram influenciados pela idade das aves. Conforme a UH os ovos de melhor qualidade se referem aos das aves com 32 e 50 semanas.The objective of this research was to evaluate production indexes for laying hens of the Lohamann lineage, under the summer and winter conditions of 2005, in São José da Mata district of Campina Grande, which is located in the Paraíban semi-arid region. 34.500 hens were used with ages of 32, 50, 60 and 71 weeks, and which were reared in four poultry houses. The production indexes (total production of eggs, egg weight, albumen weight, egg yolk weight, and rinds weight, geometric parameters (area and volume and Haugh unit of egg were calculated as a function of hen age. The production indexes in all rearing systems followed the technical recommendations. The age of the hens influenced the indexes of total production, egg weight and also egg yolk weight. The highest production of eggs was obtained with the youngest birds. On the other hand, egg weight and the egg yolk weight were obtained with the oldest birds. The geometric parameters (volume and area were not influenced by the age of the hens. According to Haugh unit index, the best evaluated hen eggs were the ones that were between 32 and 50 weeks.

  18. AVALIAÇÃO BIOQUÍMICA DO ÁCIDO SIÁLICO COMO BIOMARCADOR TUMORAL EM NOVILHAS CRIADAS EM PROPRIEDADE ENDÊMICA PARA HEMATÚRIA ENZOÓTICA DOS BOVINOS

    Directory of Open Access Journals (Sweden)

    MARCOS COELHO DE CARVALHO

    2011-06-01

    Full Text Available Pteridium aquilinum (bracken fern is one of the most concerning toxic vegetables in many countries in the world. Therefore, science aims to find ways for the epidemiological control of bovine intoxication with the purpose of making early diagnoses of Bovine Enzootic Hematuriae. Researches have shown a high correlation between the excessive production of mucoprotein by animals with Bovine Enzootic Hematuriae and sialic acid, proving that this acid acts as an inflammatorybiochemical marker associated to bladder tumor development. Based on this information, serum sialic acid levels of clinically healthy Nellore heifers (control group were statistically compared to Nellore heifers raised in bracken fern endemic properties (experimental group. The average level of serum sialic acid of the control groups was not different from the sialic acid level of the affected group (0.611 ± 0.225 g/L and 0.615 ± 0.258 g/L, respectively. Results obtained in the present study demonstrate no differences of the sialic acid average levels between the groups. Therefore, measurement ofsialic acid serum levels was not effective as a neoplasic biochemical marker in Nellore heifers continuously fed with bracken fern.

  19. Mortalidade por "septicemia dos peixes tropicais" em tilápias criadas em consorciação com suínos Edwardsiella septicemia mortality in tilapia-integrated with pig in fish farming

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    M.C.S. Muratori

    2001-12-01

    Full Text Available Com o objetivo de estudar a variação sazonal da septicemia dos peixes tropicais provocada por Edwardsiella tarda foram observadas 50 tilápias (Oreochromis sp. no verão, 45 no outono, 55 no inverno e 105 na primavera. Os peixes doentes apresentavam opacidade de córnea, dificuldade respiratória, nado desordenado, nódulos nas brânquias e lesões hemorrágicas na pele e nadadeiras. Após três dias de observação as tilápias morriam naturalmente. Nas necrópsias foi encontrado ascite com distensão da cavidade celomática e lesões hemorrágicas e necróticas no fígado, baço e rins. No interior do tubo digestivo observou-se enterite hemorrágica. Isolou-se Edwardsiella tarda a partir de provas positivas para H2S, lisina, indol, motilidade e glicose com gás. Os índices de mortalidade foram: na primavera 69,9%, no inverno 63,6%, no verão 48,0% e no outono 40,0%.A rural property was observed for one year to obtain seasonal observations of Edwardsiella septicemia in tilapias (Oreochromis sp. from an integrated fish farm (using pig excrements as food. Fifty tilapias on summer, forty-five on autumn, fifty-five on winter and one hundred and five on spring were collected and evaluated microbiologically for the presence of Edwardsiella tarda. Samples of the external surface (skin, gills and fin, intestines and muscle were analyzed. Fishes were transported alive to the laboratory, and maintained for five days for observation. The external signs observed were opacity of cornea, respiratory difficulty, disordered swimming, nodules on gills, hemorrhagic lesions under the skin and fins and incoordination of the posterior part of the body. In general, after three days of observation, the tilapias died. At necropsy, ascites with the distension of the celomatic cavity, hemorrhagic and necrotic lesions in the liver, spleen and kidneys were found. Hemorrhagic enteritis was observed in the gut tube. Edwardsiella tarda isolated presented the following biochemical profile: negative urea, sucrose, phenylalanine desaminase and malonate utilization, and positive indole, methyl red, H2S, motility, glucose with gas production and lysine decarboxylase. The mortality rates were 69.9% on spring, 63.6% on winter, 48.0% on summer and 40.0% on autumn, highest rates occurring on spring and winter (P<0,05.

  20. Perfil hematológico de cabras Saanen e mestiças (1/2 Saanen e 1/2 Anglo-nubiana criadas em clima tropical do Ceará

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    Priscila T. Souza

    2015-01-01

    Full Text Available Objetivou-se verificar a influência das condições climáticas sobre o perfil hematológico de cabras Saanen e mestiça (1/2 Saanen x 1/2 Anglo-nubiana, bem como determinar valores de referência para esses animais criados no Ceará. Foram utilizadas 30 cabras que tiveram o sangue coletado mensalmente durante a época chuvosa (fevereiro a maio e seca (agosto a novembro para realização do eritrograma e leucograma. As médias foram comparadas pelo teste t-Student e Mann Whitney, quando constatada distribuição paramétrica e não paramétrica dos dados, respectivamente, com significância quando p0,05. As hemácias e neutrófilos segmentados apresentaram uma correlação negativa com a temperatura ambiente, porém positiva com a umidade do ar e a temperatura retal (p<0,05. O hematócrito correlacionou-se positivamente com a temperatura ambiente e frequência respiratória (p<0,05. Os leucócitos e linfócitos mostraram uma correlação negativa com a temperatura retal (p<0,05. Conclui-se, portanto, que fêmeas Saanen sofrem mais influência das variações climáticas e a época chuvosa tem maior impacto negativo sobre os parâmetros hematológicos. Os valores obtidos poderão servir como referência para esses genótipos no Ceará.

  1. Perfil bioquímico de algumas enzimas no plasma sangüíneo de potras da raça Brasileiro de Hipismo (BH criadas em Colina, Estado de São Paulo

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    Fernando José Benesi

    2009-08-01

    Full Text Available Com o propósito de avaliar o perfil bioquímico de algumas enzimas, em potras sadias da raça BH (Brasileiro de Hipismo, utilizaram-se 380 amostras de plasma sangüíneo colhidas de 19 animais desde o nascimento até 24 meses de vida. Na análise dos resultados evidenciou-se que os valores médios das enzimas FA (fosfatase alcalina e CK (creatina quinase foram máximos entre o nascimento e 24 horas de vida (FA-1995.50 UI/ ; CK-189.13UI/L, enquanto que para a LD (lactato desidrogenase e GGT (gama glutamiltransferase as maiores magnitudes ocorreram, respectivamente, entre 3 e 4 dias (LD-479.11UI/L e aos 10 dias de idade (GGT-18.70UI/L. As FA, CK, LD e GGT, mostraram diminuições dos valores médios, respectivamente, até 6 meses (FA-323.50UI/L, 20 dias (CK-51UI/ L, 19 meses (LD-214.00UI/L e 4 meses (GGT-11.40UI/L estabilizando-se a seguir, com pequenas variações. A atividade da AST (aspartato aminotransferase que foi mínima logo após o nascimento (AST- 43.38UI/L, aumentou até os sete dias de vida (AST-110.89UI/L, e a seguir diminuiu progressivamente, com pequenas oscilações, até o final do estudo. Todas as enzimas avaliadas sofreram variações influenciadas pelo fator etário, particularmente no período inicial de vida dos animais estudados.

  2. UTILIZAÇÃO DE DIFERENTES ESTRUTURAS DE VARIÂNCIA RESIDUAL EM MODELOS DE REGRESSÃO ALEATÓRIA PARA DESCRIÇÃO DA CURVA DE CRESCIMENTO DE PERDIZES (Rhynchotus rufescens CRIADAS EM CATIVEIRO

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    Patrícia Tholon

    2008-01-01

    Full Text Available Random regression models (RRM allows considering heterogeneous residual variances to describe the growth for each age. However, this feature increases the number of parameters to be estimated in the maximization likelihood function process. Searching for more parsimonious RRM, several approaches have been suggested. One of them is the use of different structures of residual variances modelled through step function in different classes with similar variance or through variance functions. A total of 7,369 records of body weight of partridges, measured from birth to 210 days of partridges born from 2000 to 2004 were used in this research. The random regression models applied to the data set considered different structures of residual variances and were performed by the restricted maximum likelihood method. The residual variances were modeled using classes of 210 (R210 and 30 (R30 ages and variance functions with orders ranging from quadratic (VF2 to nine (VF9. The R30 considered birds weighted in the same week. The random effects included were the genetic additive direct and the permanent environment effects of the animal. It was not possible to include the maternal effects in the models. All random effects were modelled by sixth order regression on Legendre polynomials. The models were compared by the likelihood ratio test, the Akaike's information criterion and the Schwarz's Bayesian information criterion. Best results were showed by the models R210 and VF5. In conclusion, the most parsimonious model was VF5 and should be applied to fit growth records of partridges.

  3. Association of acepromazine with propofol in giant amazon turtles Podocnemis expansa reared in captivity Associação da acepromazina com propofol em tartarugas-da-amazônia Podocnemis expansa criadas em cativeiro

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    José Roberto Ferreira Alves-Júnior

    2012-08-01

    Full Text Available PURPOSE: To evaluate the effects of different concentrations of an anesthetic association in giant amazon turtles (Podocnemis expansa. METHODS: Twenty healthy P. expansa of both sexes weighing between 1.0 and 1.5kg commercially bred in the Araguaia River Valley, Goias, Brazil, were separated into two groups (G1 n=10 and G2 n=10. Each group received a respective protocol: P1= acepromazine (0.5 mg/kg IM and propofol (5 mg/kg IV and P2 = acepromazine (0.5 mg/kg IM and propofol (10 mg/kg IV. The acepromazine was administered in the left thoracic member and the propofol in the cervical vertebral sinus. Assessments were made of the anesthetic parameters of locomotion, muscle relaxation, response to pain stimuli in the right thoracic and pelvic members and heartbeat. RESULTS: The anesthetic induction time was the same for both protocols (P1 and P2; however the P2 effects were of a longer duration. CONCLUSION: The sedation achieved with both protocols (P1 and P2 were satisfactory for the biological sample collection, physical examinations and minor surgeries on this species.OBJETIVO: Avaliar os efeitos de uma associação anestésica com diferentes concentrações em tartarugas-da-amazônia (Podocnemis expansa. MÉTODOS: Vinte P. expansa, hígidas, de ambos os sexos, com massa corporal entre 1,0 e 1,5 kg, de um criatório comercial localizado no vale do rio Araguaia, Goiás, Brasil, foram distribuídas em dois grupos (G1 n=10 e G2 n=10. Cada grupo recebeu um protocolo sendo: P1 = acepromazina (0,5 mg/kg IM e propofol (5 mg/kg IV e P2 = acepromazina (0,5 mg/kg IM e propofol (10 mg/kg IV, aplicados nos grupos G1 e G2, respectivamente. A acepromazina foi aplicada no membro torácico esquerdo e o propofol no seio vertebral cervical. Foram avaliados os parâmetros anestésicos: locomoção, relaxamento muscular, resposta aos estímulos dolorosos no membro torácico direito e nos membros pelvinos e frequência cardíaca. RESULTADOS: O tempo de indução anestésica foi o mesmo para ambos os protocolos (P1 e P2, porém o P2 apresentou efeitos mais duradouros. CONCLUSÃO: As sedações obtidas por esses protocolos (P1 e P2 foram satisfatórias para a colheita de amostras biológicas, exames físicos e realização de pequenos procedimentos cirúrgicos nesta espécie.

  4. La globalización de los roles de género en las representaciones fílmicas de L.A.: señoras blancas, criadas latinas

    OpenAIRE

    Fernández Rodríguez, Carolina

    2013-01-01

    The aim of this paper is to analyse the concept of globalization taking into account two aspects which are often disregarded. First, the fact that globalization is very often inseparable from territorial concretion (and not spatial neutralization, as it is often maintained). Second, the fact that for women it may imply the reproduction of gender discrimination (man/woman) in the relationship between First World Women and Third World Women. The city of Los Angeles, the most photographed city i...

  5. Crescimento e desempenho de linhagens de aves pescoço pelado criadas em sistema semi-confinado Growth and performance of naked neck broiler reared in free-range system

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    Leilane Rocha Barros Dourado

    2009-06-01

    Full Text Available Este trabalho foi realizado para avaliar o crescimento e desempenho de duas linhagens de aves pescoço pelado. Foram utilizadas 400 aves, distribuídas em um delineamento inteiramente casualizado, os tratamentos consistiram de um esquema fatorial 2 x 2 (linhagens x sexo, com cinco repetições de 20 aves cada. Foram utilizadas aves Pescoço Pelado das linhagens Sasso e ISA Label. As aves receberam rações formuladas à base de milho e de farelo de soja para atender às exigências nutricionais. Foi determinado o ganho de peso (GP, o consumo de ração (CR e a conversão alimentar (CA aos 28, 56 e 84 dias de idade e o rendimento de carcaça e de partes aos 84 dias de idade. Não foi observada interação entre sexo e linhagem (P>0,05 para nenhuma das variáveis de desempenho analisadas. Para CR e GP houve diferenças significativas (PA trial was carried out to evaluate the growth and performance of naked neck birds. Four hundred birds were distributed into four treatments in factorial arrangement (2 strains x 2 genders, with five replicates of 20 birds each. The naked neck strains evaluated were Sasso and ISA Label. The birds were fed a diet formulated with corn and soybean meal according to the nutritional requirements. Food intake, body weight gain and food conversion were determined at 28, 56, and 84 days as well as the yield of carcass and cuts at 84 days. No interaction was observed between gender and strain (P>0.05. Food intake and body weight gain were different (P <0.05 between the strains and between the genders. Sasso strain presented higher performance than ISA Label. The parameters estimated for Gompertz equation, the weigh at maturity (Wm and time that the growth rate is maximum (t* were higher and maturity rate (b was lower for Sasso strain than those of Isa Label strain. These results indicated that the Isa Label was more precocious than Sasso strain. The females showed better breast yield while the males showed better thigh and drumstick yield.

  6. Toxicity of neem oil to Bemisia tabaci biotype B nymphs reared on dry bean Toxicidade de óleo de nim para ninfas de Bemisia tabaci biótipo B criadas em feijoeiro

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    Patricia Valle Pinheiro

    2009-04-01

    Full Text Available The objective of this work was to determine the most susceptible nymphal stage of Bemisia tabaci biotype B to neem (Azadirachta indica A. Juss. oil applied to dry bean (Phaseolus vulgaris L. in a screenhouse. A solution of commercial oil (Dalneem extracted from neem seeds was sprayed directly on each nymphal instar at 0, 0.1, 0.25, 0.5, 1 and 2% concentrations for lethal concentration (LC determination, and at 0, 0.5 and 1% concentrations for lethal time (LT determination. The number of living and dead nymphs was recorded five days after spraying for LC determination, and daily during six days for LT determination. The LC50 estimated for fourth instar nymphs occurred at 0.56% concentration. For all instars, LC50 and LC95 were estimated at 0.32 and 2.78% concentrations, respectively. The estimated values of LT50 at 1% concentration were 2.46, 4.45, 3.02 and 6.98 days for the first to fourth instars, respectively. The LT50 occurred at five days for 0.5% and at four days for 1% concentration in all instars. A mortality rate of over 80% was observed on the 6th day for the first to third instars at 1% concentration. The first three nymphal stages were more susceptible to neem oil when compared to the fourth nymphal stage.O objetivo deste trabalho foi determinar o estágio ninfal de Bemisia tabaci biótipo B mais suscetível ao óleo de nim (Azadirachta indica A. Juss. aplicado em feijoeiro (Phaseolus vulgaris L., em casa telada. Foram avaliados o tempo letal (TL e concentração letal (CL do óleo comercial de sementes de nim Dalneem. Para CL, concentrações de 0, 0,1, 0,25, 0,5, 1 e 2% do produto foram pulverizadas diretamente sobre as ninfas em cada ínstar. Para TL, o produto foi avaliado a 0, 0,5 e 1% de óleo de nim em cada ínstar. Ninfas vivas e mortas foram contadas cinco dias após a pulverização para CL e diariamente para TL durante seis dias. Para o quarto ínstar, a CL50 foi de 0,56% de óleo de nim. Considerando todos os ínstares, CL50 e CL95 foram estimadas em 0,32 e 2,78% de óleo de nim, respectivamente. Os TL50 para 1% de nim foram estimados em 2,46, 4,45, 3,02 e 6,98 dias para o primeiro, segundo, terceiro e quarto ínstares, respectivamente. Os TL50 estimados para 0,5 e 1% de óleo de nim foram de cinco e quatro dias, respectivamente, considerando todos os ínstares. No sexto dia, foi observada mortalidade superior a 80% para o primeiro, segundo e terceiro ínstares a 1% de óleo de nim. Os três primeiros ínstares foram mais suscetíveis ao óleo de nim que o quarto ínstar.

  7. Morfometria do trato digestório da tartaruga-da-Amazônia (Podocnemis expansa criada em sistema comercial Digestive tract morphometric of tartaruga-da-Amazônia (Podocnemis expansa kept in commercial captive systems

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    Vera Lúcia Ferreira Luz

    2003-02-01

    Full Text Available Estudos de parâmetros morfométricos do trato gastrointestinal (TGI são necessários para o conhecimento dos processos digestórios dos alimentos no organismo animal e indicar a preferência alimentar de uma espécie. Foram amostrados, bimensalmente, 100 filhotes capturados aleatoriamente em oito criadouros no município de Diorama, Goiás. O experimento foi conduzido com animais entre 23 a 29 meses de idade, que tiveram seus desempenhos avaliados por medidas biométricas do comprimento retilíneo da carapaça e do peso. Para o cálculo das relações corporais dos órgãos tomou-se como base o peso vivo individual de cinco tartarugas, em cada idade estudada, perfazendo um total de quatro colheitas com 20 exemplares de cada criadouro. As análises de comparação de médias foram realizadas pelo teste de Duncan. Os resultados indicaram que o estômago representou maior percentual do trato digestório, com 44,20%, seguido pelo intestino delgado, 28,48%, e o intestino grosso, com 20,93%, baseados na relação corporal com o TGI vazio. O TGI cheio apresentou comprimento médio de 72,75 cm. Para o intestino delgado foi obtida a média de 46,68 cm e para o intestino grosso 14,00 cm. As análises das relações corporais indicaram que o estômago e o intestino delgado apresentaram maior capacidade de armazenamento, sugerindo que desempenham importante função na digestão de alimentos consumidos.It is necessary to carry out a study that focuses on the morphometrical parameters of the gastrointestinal tract (TGI, which can provide subsidies for getting knowledge about the digestive processes that happen into the animal organism. Bimontly, it was sampled 100 P. expansa hatchlings, which were randomly captured from eight commercial flocks located at the county of Diorama, State of Goiás/Brazil. The experiment was conducted with animals of 23 to 29 months of ages, from which the biometrical measures were taken, in order to evaluate their growth performances. It was taken two biometrical measures, that is, the carapace rectlineal length - in milimeters, and the weight gain- in grams. Aiming at the calculation of the body relationships, it was taken, as a basis, the individual gross weight of five hatchlings, from each one of the studied ages, totalizing an amount of four crops of 20 specimens from each involved commercial flock. The comparison analyses of the averages were accomplished by Duncan test. Based upon the relations between the empty TGI and the whole body, the results pointed out that the stomach corresponded to the major portion of the digestive tract, that reached 44.20% of it. Second, came the small gut, that reached 28.48% of it, followed by the large gut, that reached 20.93% of the digestive tract. The full TGI showed a mean lenght of 72.75 cm. As for the small gut, it was obtained an average length of 46.68 cm and, as for the large gut, it was observed an average lenght of 14.00 cm. The body relation analyses indicated that the stomach and the small gut showed the best storage capacities and, this result suggests that those viscera play an important role in the food digestion.

  8. Características da lactação de vacas Hereford criadas em um sistema de produção extensivo na região da campanha do Rio Grande do Sul

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    Pimentel Marcelo Alves

    2006-01-01

    Full Text Available A produção de leite de vacas Hereford primíparas e multíparas manejadas sobre campo nativo foi estudada utilizando-se 144 lactações em um período de quatro anos (1995, 1998, 2000, 2001. A estimativa da produção de leite foi realizada em intervalos de 21 dias pela técnica da pesagem antes e após a mamada, do nascimento à desmama (189 dias. Foram considerados os fatores fixos ano, ordem de parto, prenhez, sexo do bezerro e raça do touro. As vacas multíparas produziram mais leite (8,27+0,26 que as primíparas (5,81+0,25 kg dia-1 no pico de lactação (42 dias. A produção total de leite em multíparas também foi superior (158,34+25,51 à das primíparas (915,44+24,47 kg. A produção de leite não foi influenciada pelos demais fatores. A maior produção de leite durante o pico de lactação consiste na principal diferença entre as curvas de lactação de vacas multíparas e primíparas, que, no entanto, apresentam amplitude de produção de leite similar nas condições estudadas. O leite fornece a energia suficiente para atender às exigências de mantença e ganho dos bezerros durante os dois primeiros meses da lactação e, apenas para mantença, até o quarto mês de lactação.

  9. Desempenho de cutias (Dasyprocta prymnolopha criadas em cativeiro do nascimento até o desmame em Teresina, Piauí Performance of agouti (Dasyprocta prymnolopha bred in captivity according to sex and parturition in Teresina, Piauí

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    João Batista Lopes

    2004-12-01

    Full Text Available Objetivou-se avaliar a influência do sexo e do tipo de parto (simples ou duplo sobre peso, comprimento corporal, perímetro torácico ao nascer e ao desmame de cutias, bem como o ganho de peso total e o ganho médio diário, em busca de subsídios que contribuam para a preservação e a exploração racional desses animais. Foram avaliados 41 animais (26 machos e 15 fêmeas, em delineamento experimental inteiramente casualizado em esquema fatorial 2x2, associando-se os tratamentos: sexo (macho, fêmea e tipo de parto (duplo e simples. O peso médio, o perímetro torácico médio, o comprimento corporal dos filhotes ao nascer para, machos e fêmeas, foram de 147,62 g e 146,25 g; 12,26 cm e 12,17 cm; 19,91 cm e 19,39 cm, respectivamente. Para as mesmas variáveis, os valores ao desmame para machos e fêmeas foram 481,92 g e 509,33 g; 17,12 cm e 16,85 cm; 30,07 cm e 30,42 cm, respectivamente. Não houve diferença significativa entre os parâmetros avaliados, quando comparados ao sexo e ao tipo de parto (simples ou duplo. Concluiu-se que o peso de filhotes de cutia ao nascer e ao desmame não tem relação com o sexo e o tipo de parto; o peso médio, o comprimento corporal e o perímetro torácico de filhotes de cutia ao nascer e ao desmame, respectivamente, são, para ambos os sexos, em torno de 145 g e 500 g, 20 e 30 cm e 12 e 17 cm; o ganho de peso médio diário do nascer ao desmame é de 7,76 g.This experiment was carried out to evaluate the effect of sex and parturition type (simple or double on live weight, body length, thoracic diameter at birth and at weaning of agouti, as well as total weight gain and daily mean weight gain, to obtain subsidies that contribute to preservation and rational exploration of these animals. Fourty-one animals (26 males and 15 females were allotted to a completely randomized a design. Animal weight, thoracic diameter and body length of agouti at birth, for males and females, were respectively: 147.62 g and 146.25 g; 12.26 cm and 12.17 cm; 19.91 cm and 19.39 cm. For the same variables, the values at wean for males and females were, respectively: 481.92 g and 509.33 g; 17.12 cm and 16.85 cm; 30.07 cm and 30.42 cm. No significant difference among the studied variables was observed, when compared to the sex and parturition type (simple or double. It was concluded that the variables studied of of agouti at birth and at weaning were affected neither by sex and nor by parturition type; animal weight, body length and thoracic diameter of agouti at birth and weaning were around 145 g and 500 g, 20 and 30 cm and 12 and 17 cm, respectively; and daily mean weight gain from to birth to weaning is about 7.76 g for both sexes.

  10. Temperatura no desenvolvimento e na reprodução de cochonilhas criadas sobre abóboras Temperature in the development and reproduction of scales reared on squash

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    Lília de Lima Andrade

    2008-12-01

    Full Text Available O desenvolvimento de insetos é dependente da temperatura. Nesta pesquisa, foi verificada a influência da temperatura no desenvolvimento e na reprodução de Chrysomphalus aonidum (L. em câmaras climatizadas com UR do ar 70±10%, fotofase de 12h e temperaturas constantes de 17, 19, 21, 23, 25 e 27 ± 1°C, tendo como substrato abóboras "cabotiá" (Curcubita maxima x Curcubita moschata var. Tetsukabuto. Foi observada a influência da temperatura nos parâmetros biológicos da cochonilha, sendo que temperaturas entre 23 e 27°C foram as mais adequadas ao inseto, propiciando menor duração do período ninfal e maior produção diária de ninfas por fêmea. Temperaturas entre 17 e 19°C provocam maior duração na fase ninfal, maior longevidade das fêmeas e menor produção diária de ninfas por fêmea.The development of the insects is dependent on the temperature. In this research, it was verified the influence of the temperature in the development and reproduction of the Chrysomphalus aonidum under climate controlled conditions (RH 70±10%, photophase of 12h, and seven constant temperatures of 17, 19, 21, 23, 25, and 27 ± 1°C; on "cabotiá" squash (Curcubita maxima x Curcubita moschata var. Tetsukabuto substrate. The temperature had influence in the biological parameters of the scale. The temperatures between 23 and 27°C were the most adequate to the scale, causing smaller duration of the nymphal period, and higher daily production of nymphs per female. At the temperatures between 17 and 19°C, the scale presented higher duration in the nymphal phase, and longevity of the females and smaller daily production of nymphs per female.

  11. Aproveitamento de alimentos de origem animal pela tartaruga-da-amazônia: Podocnemis expansa criada em cativeiro The use of animal feed in the diet of captive Arrau sideneck: Podocnemis expansa

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    Cauê Guion de Almeida

    2009-03-01

    Full Text Available Foi realizado um trabalho para determinar o aproveitamento alimentar da farinha de carne e ossos (FCO, farinha de vísceras de aves (FVA e farinha de peixe (FP em tartaruga-da-amazônia, por meio dos coeficientes de digestibilidade aparente (CDA da matéria seca (MS, proteína bruta (PB, extrato etéreo (EE e energia bruta (EB. Os animais experimentais foram 136 filhotes provenientes do Projeto Quelônios da Amazônia, no estado do Mato Grosso, mantidos em caixas com renovação de água e temperatura média de 29ºC. Os CDA foram determinados com dietas contendo 0,1% do marcador óxido de crômio III (Cr2O3. Os CDA da MS, PB, EE e EB foram, respectivamente, de 79,10; 87,61; 93,83 e 79,61% para FCO; 92,45; 94,89; 96,55 e 92,71% para FV e 93,53; 95,13; 94,05 e 93,18% para FP. Os melhores coeficientes foram obtidos com a farinha de peixe e a farinha de vísceras de aves.The effect of animal feed was studied in the Arrau sideneck, by the evaluation of apparent digestibility coefficient (ADC of the dry matter (DM, crude protein (CP, ether extract (EE and gross energy (GE of the meat and bone meal (MBM, poultry by-products meal (PBM and fish meal (FM. The turtles used were 136 hatchlings from Projeto Quelônios da Amazônia, State of Mato Grosso. Experimental animals were raised in plastic containers, with continuous water flow and mean temperature kept at 29ºC. ADC was determined by the use of diets labeled with 0.1% of chromium oxide III (Cr2O3. The ADC of DM, CP, EE and GE were, respectively, 79.10, 87.61, 93.83 and 79.61% for MBM, 92.45, 94.89, 96.55 and 92.71% for PBM and 93.53, 95.13, 94.05 and 93.18% for FM. The best coefficients were obtained with the fish meal and poultry by-products meal.

  12. The Effect of Flunitrazepam (Rohypnol(®) ) on the Development of Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) and its Implications for Forensic Entomology.

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    Baia, Tainá Costa; Campos, Alessandra; Wanderley, Bruno Mattos Silva; Gama, Renata Antonaci

    2016-07-01

    This study investigated the potential effects of flunitrazepam (known as "date rape drug") on the developmental cycle of Chrysomya megacephala, an important forensic species, and their possible implications for the calculation of the PMI. A 1050 C. megacephala eggs were divided into five groups with seven replications each. The eggs were placed on artificial diet prepared with four drug concentrations of flunitrazepam (4, 8, 16, and 32 ng/g), besides the control group (prepared with water). Were evaluated the potential effects on development time, weight gain, and mortality during the cycles. The drug had no significant effect on development time or mortality although it did affect the weight of the pupae and adults (Kruskal-Wallis, p < 0.05). The result can be deduced that the determination of the postmortem interval is not affected.

  13. Evaluating the Effect of Sarconesiopsis magellanica (Diptera: Calliphoridae Larvae-Derived Haemolymph and Fat Body Extracts on Chronic Wounds in Diabetic Rabbits

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    Jennifher Góngora

    2015-01-01

    Full Text Available We evaluated extracts taken from S. magellanica third instar larvae fat body and haemolymph using a diabetic rabbit model and compared this to the effect obtained with the same substances taken from Lucilia sericata larvae. Alloxan (a toxic glucose analogue was used to induce experimental diabetes in twelve rabbits. Dorsal wounds were made in each animal and they were infected with Staphylococcus aureus and Pseudomonas aeruginosa. They were then treated with haemolymph and lyophilized extracts taken from the selected blowflies’ larvae fat bodies. Each wound was then evaluated by using rating scales and histological analysis. More favourable scores were recorded on the PUSH and WBS scales for the wounds treated with fat body derived from the larvae of both species compared to that obtained with haemolymph; however, wounds treated with the substances taken from S. magellanica had better evolution. Histological analysis revealed that treatment led to tissue proliferation and more effective neovascularisation in less time with both species’ fat body extracts compared to treatment with just haemolymph. The results suggest the effectiveness of the substances evaluated and validate them in the animal model being used here as topical agents in treating chronic wounds.

  14. Volume of larvae Is the most important single predictor of mass temperatures in the forensically important Calliphorid, Chrysomya megacephala (Diptera: Calliphoridae)

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    Gruner, S. V.; Slone, D.H.; Capinera, J.L.; Turco, M. P.

    2017-01-01

    Calliphorid species form larval aggregations that are capable of generating heat above ambient temperature. We wanted to determine the relationship between volume, number of larvae, and different combinations of instars on larval mass heat generation. We compared different numbers of Chrysomya megacephala (F.) larvae (40, 100, 250, 600, and 2,000), and different combinations of instars (∼50/50 first and second instars, 100% second instars, ∼50/50 second and third instars, and 100% third instars) at two different ambient temperatures (20 and 30 °C). We compared 13 candidate multiple regression models that were fitted to the data; the models were then scored and ranked with Akaike information criterion and Bayesian information criterion. The results indicate that although instar, age, treatment temperature, elapsed time, and number of larvae in a mass were significant, larval volume was the best predictor of larval mass temperatures. The volume of a larval mass may need to be taken into consideration for determination of a postmortem interval.

  15. Revision of the frog fly genus Caiusa Surcouf, 1920 (Diptera, Calliphoridae), with a note on the identity of Plinthomyia emimelania Rondani, 1875.

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    Rognes, Knut

    2015-04-30

    The Oriental, Australasian and Oceanian genus Caiusa Surcouf, 1920 is revised, species concepts being based on male and female genitalia. A key to males for all known species, and a key to females for all except one are given. All relevant types still in existence have been studied, complete synonymies given and the geographical distribution reconsidered. The eight species included in the genus are: Caiusa borneoensis sp. nov. (Malaysia, Thailand, Vietnam); Caiusa coomani Séguy, 1948 (China, Malaysia, Singapore, Thailand, Vietnam); Caiusa indica Surcouf, 1920 (Australia, Cambodia, India, Indonesia, Malaysia, Papua New Guinea, Philippines, Singapore, Solomon Islands, Sri Lanka, Thailand, Vietnam); Caiusa karrakerae sp. nov. (Malaysia, Thailand); Caiusa kurahashii sp. nov. (Indonesia, Japan, Philippines); Caiusa pooae sp. nov. (Thailand); Caiusa testacea Senior-White, 1923 (India, Nepal, Sri Lanka) and Caiusa violacea Séguy, 1925, stat. rev. (Cambodia, China, Laos, Malaysia, Taiwan, Thailand, Vietnam). A lectotype is designated for Caiusa indica to fix the interpretation of the name. Caiusa nigronitens Senior-White, 1923, syn. nov. and Caiusa surcoufi Bezzi, 1927, syn. nov. are established as junior synonyms of Caiusa indica. Caiusa violacea is correctly diagnosed and errors in the original description of the female holotype are pointed out. Caiusa dubiosa Villeneuve, 1927 is established as a junior synonym of C. violacea, syn. nov. Seven Caiusa species have been reared from the egg mass of various species of frogs. The reproductive mode of the eighth species, i.e., C. indica, is unknown. Five species, i.e., C. borneoensis, C. coomani, C. karrakerae, C. kurahashii and C. violacea have been reared from one or more of the foam nesting frog species Chiromantis nongkhorensis (Cochran, 1927), Polypedates leucomystax (Gravenhorst, 1927), Polypedates megacephalus Hallowell, 1861, Rhacophorus annamensis Smith, 1924, Rhacophorus dulitensis Boulenger, 1892, Rhacophorus kio Ohler & Delorme, 2005 and Rhacophorus owstoni (Stejneger, 1907) all belonging in the family Rhacophoridae in Anura. These five Caiusa species all have a specialised ovipositor tip, with small spine-like setae on the ST8 and the hypoproct, probably enabling the flies to oviposit on a foam nest with a hardened outer surface. They form a monophyletic group on account of these features of the ovipositor, unique in the Oestroidea. The sixth species, C. testacea, has been reared from a frog egg mass, the frog species being unknown. Its ovipositor structure is also unknown. The seventh species, C. pooae, has been reared once from the jelly-like egg mass of Feihyla hansenae (Cochran, 1927), also in Rhacophoridae. Caiusa pooae females do not have spine-like setae on the ovipositor, a fact correlated with the soft outer surface of the jelly-like egg mass on which a C. pooae female had oviposited. The extreme rarity of C. pooae oviposition on Feihyla hansenae egg masses may indicate that this fly perhaps has another, unknown, regular oviposition substrate. Caiusa pooae and C. indica make up a second monophyletic group within Caiusa. Caiusa indica, the most common and most widespread species of the genus, has an ovipositor structure similar to C. pooae. Its breeding substrate is unknown and it occurs both within and outside the distributional area of Rhacophoridae. Possibly both C. indica and C. pooae share a regular oviposition substrate that has still to be discovered. The holotype female of Plinthomyia emimelania Rondani, 1875 from Sarawak is established as a member of the genus Bengalia Robineau-Desvoidy, 1830, thus Plinthomyia Rondani, 1875 becomes a junior synonym of Bengalia Robineau-Desvoidy, 1830, syn. nov. It is removed from the synonymy of Phumosia Robineau-Desvoidy, 1830.

  16. Glutamate-gated chloride channel subunit cDNA sequencing of Cochliomyia hominivorax (Diptera: Calliphoridae): cDNA variants and polymorphisms.

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    Lopes, Alberto Moura Mendes; de Carvalho, Renato Assis; de Azeredo-Espin, Ana Maria Lima

    2014-09-01

    The New World screwworm (NWS) Cochliomyia hominivorax (Coquerel) is one of the major myiasis-causing flies that injures livestock and leads to losses of ~US$ 2.7 billions/year in the Neotropics. Ivermectin (IVM), a macrocyclic lactone (ML), is the most used preventive insecticide for this parasite and targets the glutamate-gated chloride (GLUCLα) channels. Several authors have associated altered GluClα homologues to MLs resistance in invertebrates, although studies about resistance in NWS are limited to other genes. Here, we aimed to characterise the NWS GluClα (ChGluClα) cDNA and to search for alterations associated with IVM resistance in NWS larvae from a bioassay. The open reading frame of the ChGluClα comprised 1,359 bp and encoded a sequence of 452 amino acids. The ChGluClα cDNAs of the bioassay larvae showed different sequences that could be splice variants, which agree with the occurrence of alternative splicing in GluClα homologues. In addition, we found cDNAs with premature stop codons and the K242R SNP, which occurred more frequently in the surviving larvae and was located close to mutation (L256F) involved in ML resistance. Although these alterations were in low frequency, the ChGluClα sequencing will allow further studies to find alterations in the gene of resistant natural populations.

  17. Persistence of antibiotic-resistant and -sensitive Proteus mirabilis strains in the digestive tract of the housefly (Musca domestica) and green bottle flies (Calliphoridae).

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    Wei, Ting; Miyanaga, Kazuhiko; Tanji, Yasunori

    2014-10-01

    Synanthropic flies have been implicated in the rapid dissemination of antibiotic-resistant bacteria and resistance determinants in the biosphere. These flies stably harbor a considerable number of bacteria that exhibit resistance to various antibiotics, but the mechanisms underlying this phenomenon remain unclear. In this study, we investigated the persistence of antibiotic-resistant bacteria in the digestive tract of houseflies and green bottle flies, using Proteus mirabilis as a model microorganism. One resistant strain carried the blaTEM and aphA1 genes, and another carried a plasmid containing qnrD gene. Quantitative PCR and 454 pyrosequencing were used to monitor the relative abundance of the Proteus strains, as well as potential changes in the overall structure of the whole bacterial community incurred by the artificial induction of Proteus cultures. Both antibiotic-resistant and -sensitive P. mirabilis strains persisted in the fly digestive tract for at least 3 days, and there was no significant difference in the relative abundance of resistant and sensitive strains despite the lower growth rate of resistant strains when cultured in vitro. Therefore, conditions in the fly digestive tract may allow resistant strains to survive the competition with sensitive strains in the absence of antibiotic selective pressure. The composition of the fly-associated bacterial community changed over time, but the contribution of the artificially introduced P. mirabilis strains to these changes was not clear. In order to explain these changes, it will be necessary to obtain more information about bacterial interspecies antagonism in the fly digestive tract.

  18. Effect of preservative solutions on preservation of Calliphora augur and Lucilia cuprina larvae (Diptera: Calliphoridae) with implications for post-mortem interval estimates.

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    Day, Donnah M; Wallman, James F

    2008-07-18

    A major role of forensic entomology is to estimate the post-mortem interval. An entomologist's estimate of post-mortem interval is based on a series of generally valid assumptions, error in any of which can alter the accuracy of an estimate. The initial process of collecting and preserving maggots can itself lead to error, as can the method of killing and preservation. Since circumstances exist where it is not possible to rear maggots, methods of killing and preservation can be vital to preserving the integrity of entomological evidence. In this study, a number of preservation techniques used at crime scenes and in mortuaries were examined, and their effect on feeding third-instar larvae of Calliphora augur and Lucilia cuprina evaluated. The preservatives used were 70, 75, 80, 90 and 100% EtOH, Kahle's solution and 10% formalin. Each treatment was replicated three times. The effect of handling on first- and second-instar, feeding and post-feeding third-instar larvae of C. augur was also examined and compared to unhandled controls. Finally, the effects of preservatives were noted when larvae of C. augur and L. cuprina were placed into preservatives alive. It was found that continued handling is detrimental to specimens because preservative evaporates from both the vial and the specimens. No single preservative type was found to be entirely suitable for both species if DNA retrieval is desired. Specimens placed into most preservatives alive exhibited adverse colour changes, desiccation, sunkeness and agglomeration. It is concluded that the reaction to preservative type might be species specific and that different instars of the same species might also react differently.

  19. Volume of Larvae Is the Most Important Single Predictor of Mass Temperatures in the Forensically Important Calliphorid, Chrysomya megacephala (Diptera: Calliphoridae).

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    Gruner, S V; Slone, D H; Capinera, J L; Turco, M P

    2017-01-01

    Calliphorid species form larval aggregations that are capable of generating heat above ambient temperature. We wanted to determine the relationship between volume, number of larvae, and different combinations of instars on larval mass heat generation. We compared different numbers of Chrysomya megacephala (F.) larvae (40, 100, 250, 600, and 2,000), and different combinations of instars (∼50/50 first and second instars, 100% second instars, ∼50/50 second and third instars, and 100% third instars) at two different ambient temperatures (20 and 30 °C). We compared 13 candidate multiple regression models that were fitted to the data; the models were then scored and ranked with Akaike information criterion and Bayesian information criterion. The results indicate that although instar, age, treatment temperature, elapsed time, and number of larvae in a mass were significant, larval volume was the best predictor of larval mass temperatures. The volume of a larval mass may need to be taken into consideration for determination of a postmortem interval.

  20. Dynamics of experimental populations of native and introduced blowflies (Diptera: Calliphoridae: mathematical modelling and the transition from asymptotic equilibrium to bounded oscillations

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    WAC Godoy

    1996-10-01

    Full Text Available The equilibrium dynamics of native and introduced blowflies is modelled using a density-dependent model of population growth that takes into account important features of the life-history in these flies. A theoretical analysis indicates that the product of maximum fecundity and survival is the primary determinant of the dynamics. Cochliomyia macellaria, a blowfly native to the Americas and the introduced Chrysomya megacephala and Chrysomya putoria, differ in their dynamics in that the first species shows a damping oscillatory behavior leading to a one-point equilibrium, whereas in the last two species population numbers show a two-point limit cycle. Simulations showed that variation in fecundity has a marked effect on the dynamics and indicates the possibility of transitions from one-point equilibrium to bounded oscillations and aperiodic behavior. Variation in survival has much less influence on the dynamics.

  1. Lucilia sericata strain from Colombia: Experimental colonization, life tables and evaluation of two artificial diets of the blowfly Lucilia sericata (Meigen) (Diptera: Calliphoridae), Bogotá, Colombia strain.

    Science.gov (United States)

    Rueda, Luis C; Ortega, Luis G; Segura, Nidya A; Acero, Víctor M; Bello, Felio

    2010-01-01

    The objective of this work was to establish, under experimental laboratory conditions, a colony of Lucilia sericata, Bogotá-Colombia strain, to build life tables and evaluate two artificial diets. This blowfly is frequently used in postmortem interval studies and in injury treatment. The parental adult insects collected in Bogotá were maintained in cages at 22°C±1 average temperature, 60%±5 relative humidity and 12 h photoperiodicity. The blowflies were fed on two artificial diets that were evaluated over seven continuous generations. Reproductive and population parameters were assessed. The life cycle of the species was expressed in the number of days of the different stages: egg = 0.8±0.1, larvae I = 1.1±0.02, larvae II = 1.94±0.16, larvae III = 3.5±0.54, pupae = 6.55±0.47, male adult = 28.7±0.83 and female adult = 33.5±1.0. Total survival from egg stage to adult stage was 91.2% for diet 1, while for diet 2 this parameter was 40.5%. The lifetime reproductive output was 184.51±11.2 eggs per female. The population parameters, as well as the reproductive output of the blowflies that were assessed, showed relatively high values, giving evidence of the continuous increase of the strain over the different generations and making possible its maintenance as a stable colony that has lasted for more than two years.

  2. Behavior of the combined radial post-feeding larval dispersal of the blowflies Chrysomya megacephala and Chrysomya albiceps (Diptera, Calliphoridae and implications for forensic entomology

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    Leonardo Gomes

    2007-03-01

    Full Text Available The aims of this work was to study the life stages of Chrysomya megacephala in a circular arena allowing the combined radial post-feeding dispersal from the center of the arena of C. albiceps and C. megacephala larvae. To determine the location of each pupa, the distance from the center, the depth and weight of each pupa formed were analyzed. For the larvae of C. albiceps, females released buried deeper than males, reaching an average depth of 10.74 cm and C. megacephala larvae presented greater average results than C. albiceps for all variables, particularly distance, which was 16.02 cm for this species and 12.30 cm for C. albiceps. C. albiceps larvae preyed on 30 C. megacephala larvae in both experiments. These results could be used in forensic entomology because the evaluation of Post Mortem Interval (PMI was one of the most important aspects of legal medicine, and it could be underestimated if the older dispersing larvae or those that dispersed longer and faster or deeper, were not taken into account.O objetivo desse trabalho foi estudar estágios de vida de Chrysomya megacephala em uma arena circular permitindo a dispersão larval pós-alimentar combinada a partir do centro das larvas de C. megacephala e C. albiceps. Para determinar a localização de cada pupa a distância a partir do centro, a profundidade e o peso foram coletados. Do total de larvas soltas as fêmeas de C. albiceps se enterraram mais profundamente que os machos, alcançando uma profundidade de 10,74 cm. Apesar dessa diferença, as larvas de C. megacephala apresentaram resultado em média maiores para todas a variáveis, particularmente a distância, a qual foi 16,02 cm para essa espécie e 12,30 para C. albiceps. Além disso, as larvas de C. albiceps predaram 30 larvas de C. megacphala durante esse processo em ambos os experimentos. Esses estudos podem ser utilizados em Entomologia forense porque a estimativa do Intervalo Pós Morte (IPM é um dos aspectos mais importantes em medicina legal e a não consideração das larvas que dispersam mais longe ou se enterram mais podem levar a uma subestimativa do IPM.

  3. Estudo comparado do desenvolvimento pós-embrionário de Cochliomyia macellaria (Fabricius (Diptera, Calliphoridae em duas dietas à base de carne, em laboratório Comparative studyy of post-embryonic development of Cochliomyia macellaria(Fabricius (Diptera, Calliphoridae under two meat diets, in laboratory

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    Sandra L. da Cunha e Silva

    1994-01-01

    Full Text Available The influence of a diet based on putrid horse meat over the post-embryonic development of Cochliomyia macellaria (Fabricius, 1775 under controlled conditions (UR 65 10% and 14 hours of photophase was compared with the results obtained using a meat broth diet to which other sources of animal and vegetable protein were added. The flies were maintained at 30ºC, from egg until mature larvae spontaneously abandoned the diet. They were then transfered to a climatized chamber at 27ºC. The larvae and pupae viability and the weight of the mature larvae were significantly inferior, when a diet based on meat broth was used, even though the larvae period was significantly increased with this diet. This type of diet did not charge the time of development of the pupae. The inoculation of the egg mass directly over the diet was recommend, instead of the technique in which the egg masses are transfered to humid filter paper, followed by the handling of the recently ecloded larvae.

  4. Associação entre larvas de Chrysomya megacephala (Fabricius e Chrysomya albiceps (Wiedemann, Chrysomya megacephala (Fabricius e Cochliomyia macellaria (Fabricius (Calliphoridae, Diptera sob condições de laboratório Association between Chrysomya megacephala (Fabricius and Chrysomya albiceps (Wiedemann, Chrysomya megacephala (Fabricius and Cochliomyia macellaria (Fabricius larvae (Calliphoridae, Diptera, under laboratory conditions

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    Valéria Magalhães Aguiar-Coelho

    1995-01-01

    Full Text Available This paper intends to recognize some aspects of interspecific relationships between Chrysomya megacephala (Fabricius and C. albiceps (Wiedemann and between C. megacephala and Cochliomyia macellaria (Fabricius larvae in laboratory. A diet consisting of decomposing horse flesh was used, and the relation of 1 larva/g diet was established. The development of the two species was done in pure and associated cultures. The association with Chrysomya albiceps influenced the post-embrionary development period and the weight of mature larvae of the C. megacephala, reducing their. Such weight decrease, along with the predation of larvae of C. megacephala larvae, yelded a decrease in survival in all of the stages. Larvae of this species showed an increase in its weight when associated with Cochliomyia macellaria. The native species responded to the association by reducing its body weigth and its viability. This association doesnt influence the duration of post-embrionary development.

  5. Diversidade de Calliphoridae (Insecta: Diptera na base de extração petrolífera da Bacia do Rio Urucu, na Amazônia brasileira Diversity of Calliphoridae flies (Insecta: Diptera in the oil extraction field of Rio Urucu basin, in brasilian Amazonian

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    Maria Cristina Esposito

    2010-09-01

    Full Text Available A diversidade e abundância de dípteros califorídeos de três ambientes (clareira artificial, clareira natural e mata de Porto Urucu/AM foram avaliadas em coletas anuais realizadas em 2004, 2005 e 2006. Ao longo destes três anos foram coletados 2.121 exemplares pertencentes a 14 espécies. As espécies mais abundantes foram Chloroprocta idioidea (Robineau- Desvoidy, Eumesembrinella randa (Walker e Hemilucilia semidiaphana (Rondani. Os habitats de matas e clareiras naturais apresentaram maior abundância de califorídeos quando comparados às clareiras artificiais, com índices de diversidade e equitabilidade também maiores do que em clareiras artificiais, onde a dominância foi mais elevada.The diversity and abundance of blow flies in three environments (anthropic gaps, tree-fall gaps, and primary "terra firme" forest of Porto Urucu/AM were evaluated in annual collects realized in 2004, 2005, and 2006. During these three years were collected 2,121 specimens belonging to 14 species. The most abundant species were Chloroprocta idioidea (Robineau- Desvoidy, Eumesembrinella randa (Walker, and Hemilucilia semidiaphana (Rondani. Primary forests and tree-fall gaps enviroments, showed higher blow flies abundance than anthropic gaps, with diversity and equitability index higher than anthropic gaps, when the dominance value was high.

  6. Efeito do tipo de substrato para pupação na dispersão larval pós-alimentar de Chrysomya albiceps (Diptera, Calliphoridae Effect of the substrate for pupation in the postfeeding larval dispersal of Chrysomya albiceps (Diptera: Calliphoridae

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    Leonardo Gomes

    Full Text Available As moscas-varejeiras utilizam substratos discretos e efêmeros para posturas dos ovos e para alimentação das larvas. Após a exaustão de recursos, as larvas começam a procurar por um sítio de pupação no habitat ou por mais fonte de alimento adicional (dispersão larval pós-alimentar. No entanto, o tipo de substrato de dispersão pode afetar este processo; assim, procurou-se avaliar o comportamento de dispersão de Chrysomya albiceps (Wiedemann 1819 por meio da localização das pupas em arenas circulares preenchidas com vermiculita, comparando-se com outros estudos feitos em serragem. As arenas foram dividas em 72 setores iguais a partir do centro de dispersão para facilitar a localização das pupas no substrato. A massa, a distância de dispersão do centro de arena, e a profundidade de cada pupa foram determinadas e verificou-se a relação entre peso, profundidade e distância do centro de dispersão. Pôde-se constatar que as larvas com menor massa foram as que percorreram maior distância do centro de dispersão, como também foi observado em estudos anteriores que empregaram outros substratos. O sítio de pupação da maioria dos indivíduos foi entre 2 e 8 cm de profundidade (média 4,96±2,97 cm. Tal estudo do processo de dispersão pode ser útil na estimativa do intervalo pós-morte (IPM para corpos humanos em investigações médico-criminais.Blowflies utilize discrete and ephemeral sites for breeding and larval nutrition. After the exhaustion of food, the larvae begin dispersing to search for pupation sites or for additional food sources, a process referred to as postfeeding larval dispersal. However the nature of the substrate can affect this process. Some of the most important aspects of this behavior were here investigated in Chrysomya albiceps (Wiedemann, 1819, utilizing a circular arena covered with vermiculite allowing the post-feeding larval dispersal from the center and to comparing with others studies that used sawdust. To determinate the location of each pupa, the arena was divided into 72 equal sectors from the center. For each pupa, weight, dispersal distance from the center of arena, and depth were determined. Statistical tests were performed to verify the relationship among weight, depth and distance of burial for pupation. The larvae that dispersed farthest were those with lower weights, as in other studies that employed other substrates. The majority of individuals reached the burial depth for pupation between 2 and 8 cm (mean 4.96±2.97 cm. The study of this dispersal process can be helpful in the estimation of postmortem interval (PMI for human corpses in medico-criminal investigations.

  7. Dispersão larval radial pós-alimentar em Lucilia cuprina (Diptera, Calliphoridae: profundidade, peso e distância de enterramento para pupação Postfeeding radial larval dispersion in Lucilia cuprina (Diptera, Calliphoridae: depth, weight and distance of burying for pupation

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    Leonardo Gomes

    2004-06-01

    Full Text Available Blowflies utilize discrete and ephemeral sites for breeding and larval nutrition. After the exhaustion of food, the larvae begin dispersing to search for sites to pupate or for additional food source, process referred as postfeeding larval dispersal. Some aspects of this process were investigated in Lucilia cuprina (Wiedemann, 1830, utilizing a circular arena to permit the radial dispersion of larvae from the food source in the center. To determine the localization of each pupa, the arena was split into 72 equal sectors from the center. For each pupa, distance from the center of arena, weight and depth were determined. Statistical tests were performed to verify the relation among weight, depth and distance of burying for pupation. It was verified that the larvae that disperse farthest are those with lowest weights. The majority of individuals reached the depth of burying for pupation between 7 and 18 cm. The study of this process of dispersion can be utilized in the estimation of postmortem interval (PMI for human corpses in medico-criminal investigations.

  8. Influence of photoperiod on body weight and depth of burrowing in larvae of Chrysomya megacephala (Fabricius (Diptera, Calliphoridae and implications for forensic entomology A influência do fotoperíodo no peso corpóreo e na profundidade de enterramento em larvas de Chrysomya megacephala (Fabricius (Diptera, Calliphoridae e as implicações para entomologia forense

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    Leonardo Gomes

    2006-03-01

    Full Text Available Blowflies use discrete, ephemeral breeding sites for larval nutrition. After exhaustion of the food supply, the larvae disperse in search of sites to pupate or to seek other sources of food in a process known as post-feeding larval dispersal. In this study, some of the most important aspects of this process were investigated in larvae of the blowflies Chrysomya megacephala exposed to a variety of light: dark (LD cycles (0:0 h, 12:12 h and 24:0 h and incubated in tubes covered with vermiculite. For each pupa, the body weight and depth of burrowing were determined. Statistical tests were used to examine the relationship of depth of burrowing and body weight to photoperiod at which burrowing occurred. The study of burial behavior in post-feeding larval dispersing can be useful for estimating the postmortem interval (PMI of human corpses in forensic medicine.Moscas-varejeiras usam substratos discretos e efêmeros para nutrição larval. Após a exaustão do suprimento de comida, as larvas dispersam na procura por locais para pupação na outros recursos de alimento em um processo conhecido como dispersão larval pós- alimentar. Nesse estudo, alguns dos aspectos mais importantes desse processo foram investigados em larvas de moscas-varejeiras Chrysomya megacephala expostas a uma variação de ciclos luz: escuro (LD (0:24h, 12:12h e 24:0h e incubadas em tubos cobertos com vermiculita. Para cada pupa, o peso corpóreo e a profundidade de enterramento foram determinados. Testes estatísticos foram usados para examinar a relação entre profundidade de enterramento e o peso corpóreo e o fotoperíodo a que esse enterramento ocorreu. O estudo do comportamento de enterramento na dispersão larval pós-alimentar pode ser útil para estimar o intervalo pós-morte (IPM em cadáveres humanos em medicina forense.

  9. Aspectos biológicos de Thyrinteina arnobia (Lep.: Geometriadae provenientes de lagartas criadas em folhas de Eucalyptus cloeziana ou de Psidium guajava sob condições de campo Biological aspects of Thyrinteina arnobia (Lep.: Geometridae adults originated from caterpillars reared on leaves of Eucalyptus cloeziana or Psidium guajava under field conditions

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    Anderson Mathias Holtz

    2003-12-01

    Full Text Available Os plantios de Eucalyptus no Brasil podem sofrer danos por espécies nativas de insetos de diversas ordens, como Orthoptera, Coleoptera e Lepidoptera. Esses insetos podem alimentar-se tanto de mirtáceas brasileiras como goiabeira, gabirobeira, jabuticabeira, entre outras, como de espécies do gênero Eucalyptus. Entre os desfolhadores, destaca-se Thyrinteina arnobia (Stoll (Lepidoptera: Geometridae como o mais daninho dessa ordem para a eucaliptocultura brasileira. Este trabalho teve por objetivo avaliar aspectos biológicos de adultos de T. arnobia provenientes de lagartas alimentadas com plantas de eucalipto e, ou, goiabeira. Adultos deste inseto criados em folhas de eucalipto e, ou, de goiabeira apresentaram diferenças significativas para a maioria dos aspectos biológicos avaliados, exceto para a duração dos períodos de préoviposição, de oviposição e razão sexual. Assim, insetos herbívoros que vivem em hospedeiros filogeneticamente próximos ao eucalipto são capazes de causar danos consideráveis em reflorestamentos com espécies desse grupo, o que provavelmente ocorre pelo fato de elas estarem ainda em processo de adaptação a essa praga que atacaria o eucalipto, por estar fugindo da pressão exercida por barreiras físicas e químicas existentes nas mirtáceas nativas brasileiras.Eucalyptus plantations in Brazil may be damaged by native insects of many orders including Orthoptera, Coleoptera and Lepidoptera. These insects feed on Brazilian tree species of the family Myrtaceae to which the genus Eucalyptus belongs. The Lepidoptera Thyrinteina arnobia Stoll (Lepidoptera: Geometridae is the most harmful defoliator of Eucalyptus in Brazil. The objective of this work was to evaluate biological aspects of T. arnobia adults originated from caterpillars fed on guava or eucalyptus plants. Adults of T. arnobia originated from caterpillars reared with eucalyptus or guava leaves presented significant differences for most biological aspects evaluated, except for duration of preoviposition and oviposition periods and sex ratio. This shows that species feeding on host plants related to eucalyptus can cause considerable damage in reforestation with species of this genus. T. arnobia insects are still undergoing an imposed adaptation process to eucalyptus plants and likely feed on these plants to escape from pressure by physical and chemical barriers of Brazilian native Myrtaceae.

  10. Exigências de Proteína Bruta e Energia Metabolizável para Codornas Japonesas Criadas para a Produção de Carne Crude Protein and Metabolizable Energy Requirements for Japanese Quails Reared for Meat Production

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    Newton Tavares Escocard de Oliveira

    2002-01-01

    Full Text Available Objetivando-se estimar as exigências de proteína bruta (PB e energia metabolizável (EM para máximo desempenho de codornas Japonesas de ambos os sexos, para a produção de carne, além de determinar a idade ótima de abate, 450 codornas de cada sexo, num total de 900 codornas, foram utilizadas em um delineamento experimental de blocos casualizados, com cinco repetições de seis codornas por unidade experimental. Os tratamentos foram constituídos do sexo das codornas e das dietas experimentais, resultantes das combinações de cinco níveis de PB (18, 20, 22, 24 e 26% e três níveis de EM (2800, 3000 e 3200 kcal/kg de ração, e distribuídos nas parcelas e nos quatro períodos experimentais, como subparcelas. As exigências estimadas de PB e EM durante o primeiro (5 a 16, terceiro (27 a 38 e quarto (38 a 49 dias de idade períodos foram: 26 e 2800, 18 e 3200 e 19,62% e 3200 kcal/kg ração para fêmeas e 26 e 2800, 18 e 3200 e 18% e 3200 kcal/kg ração para machos, respectivamente. A exigência de PB para fêmeas e machos no segundo período (16 a 27 dias de idade foi de 26%, entretanto, não foi possível estimar a exigência de EM para ambos os sexos, em relação ao mesmo período. No período total, a exigência protéica estimada para ganho de peso máximo de fêmeas e machos foi de 24,73%. As idades estimadas que resultaram em peso máximo dependeram do nível de PB da dieta, variando de 91 a 189 para fêmeas e de 57 a 83 dias para machos.Four hundred and fifty quails of each sex, in a total of nine hundred quails, were assigned to a completely randomized design with five replicates of six quails per experimental unit, to estimate the crude protein (CP and metabolizable energy (ME requirements for maximum performance of females and males Japanese quails reared for meat production purpose and to determine the best slaughter age. The treatments were consisted of the quails sex and the experimental diets, resultant from the combination of five levels of CP (18, 20, 22, 24 and 26% and three level of ME (2800, 3000 and 3200 kcal/kg of diet, and allotted to the plots and to the four experimental periods, as splitplots. The estimates of CP and ME requirements during the first (5 to 16, third (27 to 38 and fourth (38 to 49 days of age periods were: 26 and 2800; 18 and 3200 and 19,62% and 3200 kcal/kg of diet for females and 26 and 2800; 18 and 3200 and 18% and 3200 kcal/kg of diet for males, respectively. The CP requirement for females and males in the second (16 to 27 days of age period was 26%, however it was not possible to estimate the ME requirement for both sexes, in relation to the same period. For the whole period, the estimated crude protein requirement for maximum weight gain of females and males was 24.73%. The estimated ages for maximum body weight depended on the crude protein level of diet and varied from 91 to 189 days of age for females and from 57 to 83 days for males.

  11. Anticorpos IgG anti-Neospora caninum e Toxoplasma gondii em búfalas (Bubalus bubalis criadas no estado do Pará Occurrence of IgG antibodies anti-Neospora caninum and Toxoplasma gondii in female water buffaloes (Bubalus bubalis raised in the Brazilian state of Pará

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    Sandro P. Silva

    2010-05-01

    Full Text Available Para determinar a ocorrência de anticorpos IgG anti-Neospora caninum e Toxoplasma gondii em 14 unidades produtivas de búfalos, situadas em 13 municípios no estado do Pará, foram coletadas amostras de soro sanguíneo de 374 fêmeas adultas. Os soros foram submetidos à reação de imunofluorescência indireta (RIFI, utilizando-se os títulos 200 e 64, respectivamente como ponto de corte para N. caninum e T. gondii. Obteve-se 153 (40,9% de animais soropositivos para N. caninum com 100% das propriedades com focos da infecção, enquanto que quatro búfalas (1,1% foram soropositivas em quatro fazendas para T. gondii e uma búfala (0,27% foi soropositiva para ambos parasitos. A presença de anticorpos é um indicativo da circulação desses protozoários em búfalos das propriedades estudadas, representando uma fonte de infecção para outros animais, assim como o possível envolvimento em distúrbios reprodutivos nessa espécie.To investigate the occurrence of antibodies IgG anti-Neospora caninum and Toxoplasma gondii, blood samples were collected from 374 adult female water buffaloes originated from 14 production units located in 13 counties of the state of Pará. The sera were subjected to indirect fluorescent antibody (IFA, using titers of 200 and 64 as IFA cut off points for N. caninum and T. gondii, respectively. Hundred and fifty three (40.9% buffaloes were sero-positive for N. caninum, with 100% of the properties being sources of infection. Four buffaloes (1.1% in four farms were seropositive for T. gondii, and one buffalo cow (0.27% was seropositive for the two parasites. The presence of antibodies indicates that these protozoan parasites are circulating among buffaloes from the properties studied, representing a source of infection to other animals, as well as a possible cause of reproductive disorders in this species.

  12. Avaliação do desempenho e rendimento de carcaça de quatro linhagens de frangos de corte criadas em Goiás Performance and carcass yield of four broiler strains raised in Goiás, Brazil

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    José Henrique Stringhini

    2003-02-01

    Full Text Available Um experimento foi conduzido no Aviário Experimental do Abatedouro São Salvador, Itaberaí - GO, com o objetivo de se avaliarem os parâmetros de desempenho e qualidade de carcaça de quatro linhagens de frangos de corte. Foram avaliados o desempenho (41, 44 e 48 dias e o rendimento de carcaça (41 e 44 dias das linhagens Ross, Cobb, Arbor Acres e Avian Farms. O delineamento experimental adotado foi em blocos ao acaso num esquema fatorial 4x2 (linhagens x sexo com quatro repetições de 63 aves para cada tratamento. Os resultados desse experimento apontaram desempenho e peso de carcaça superiores dos machos quando comparados às fêmeas, contudo nas características de rendimento de carcaça se mostraram semelhantes. A linhagem Ross teve ganho de peso e peso ao abate superiores às demais linhagens aos 44 dias de idade, mas isto não ocorreu nas demais fases. Não houve diferenças entre as linhagens em termos de rendimento de carcaça ou de cortes.An experiment was carried out in the Abatedouro São Salvador, Goiás, Brazil, in order to evaluate the performance and carcass characteristics of four different broiler strains. The performance and carcass characteristics of Ross, Cobb, Avian Farms and Arbor Acres chickens were measured until 41, 44 and 48 days of age and the carcass parameters were measured at 41 and 44 days of age. Broilers were allotted in an randomized block design in a 4x2 factorial scheme (strains x sex with four replicates of 63 birds each. It was observed better productive parameters and heavier body and carcass weight for male broilers than females, although no effect of sex occurred in carcass yield. Ross birds showed heavier body weight and higher cumulative weight gain at 44 days of age, but this was not observed in the other phases tested. There were no differences in commercial parts yield and carcass characteristics between the different strains.

  13. Machos e Brothers: uma etnografia sobre o armário em relações homoeróticas masculinas criadas on-line Machos and brothers: an ethnography about the closet in male homoerotic relations created on-line

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    Richard Miskolci

    2013-04-01

    Full Text Available A partir de uma etnografia feita com homens que se apresentam como Macho ou Brother em salas de bate-papo dirigidas a um público gay e bissexual da cidade de São Paulo, este artigo explora como eles articulam plataformas de busca de parceiros com o objetivo de criar relações em segredo. Esses homens apresentam-se e buscam parceiros que se descrevem como "discretos" e "fora do meio gay", forjando relações dentro do regime de controle da sexualidade conhecido como armário. Busco expor e compreender as normas de sexualidade e gênero que regem o armário desses usuários, os quais buscam manter seu status heterossexual mesmo tendo relações amorosas e/ou sexuais com outros homens.Based on an ethnography with men who introduce themselves as Macho or Brother in chatrooms directed toward a gay and bisexual audience in the city of São Paulo, this article explores how these users articulate different platforms to create secret relationships. These men introduce themselves and search for partners that can be described as "straight acting" and "not part of the gay scene" creating relations shaped by the sexuality control regime known as the closet. This analysis tries to expose gender and sexuality norms that shape these users' closet, or the way they act trying to keep their heterosexual status while also having sexual or love relationship with other men.

  14. Natureza humana criada em laboratório: biologização e genetização do parentesco nas novas tecnologias reprodutivas Human nature created in the laboratory: the biologization and genetization of kinship in new reproductive technologies

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    Naara Luna

    2005-08-01

    Full Text Available As novas tecnologias reprodutivas, também chamadas de 'reprodução assistida', são procedimentos que permitem a procriação sem a relação sexual, sendo as técnicas mais conhecidas a inseminação artificial e a fertilização in vitro (bebê de proveta. O acesso às técnicas também levanta a discussão sobre a genetização dos laços de parentesco, em contraste com as práticas de adoção ou procedimentos como a doação de gametas (óvulos e espermatozóides e embriões. Com base em pesquisa etnográfica com usuárias e profissionais envolvidos em reprodução assistida, e em bibliografia específica, este trabalho visa discutir que concepções de natureza humana estão implicadas na biologização e na genetização do parentesco nas novas tecnologias reprodutivas.New reproductive technologies or assisted reproduction are procedures that make procreation possible without sexual relations, the most well-known being artificial insemination and in vitro fertilization ('test-tube' babies. Use of these new techniques raises the issue of the genetization of kinship ties, in contrast with adoption or procedures involving the donation of gametes (eggs and sperms and embryos. Based both on ethnographic research with users and professionals in assisted reproduction and also on bibliographic research, the article discusses the concepts of human nature implied in the biologization and genetization of kinship through these new reproductive technologies.

  15. Capacidade reprodutiva de fêmeas de Apanteles galleriae (Hymenoptera, Braconidae em lagartas de Galleria mellonella e Achroia grisella (Lepidoptera, Pyralidae criadas com dietas diferentes Reproductive capacity of Apanteles galleriae females (Hymenoptera, Braconidae in Galleria mellonella and Achroia grisella larvae (Lepidoptera, Pyralidae reared on different diets

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    Giuliano Grici Zacarin

    2004-06-01

    Full Text Available The reproductive capacity of females of Apanteles galleriae (Wilkinson, 1932 was evaluated in fifth instar caterpillars of Galleria mellonella (Linnaeus, 1758 and Achroia grisella (Fabricius, 1754 fed on standard diet and diets enriched with protein. The reproductive capacity of parasitoid females on fifth instar caterpillars of G. mellonella and A. grisella with variable weight was also evaluated. The host weight interfered in the sex ratio of the obtained parasitoids. In heavier caterpillars, the investment in female descendants was greater than in males, and in lighter caterpillars the inverse occurred.

  16. Condições térmicas ambientais e desempenho de aves criadas em aviários com e sem o uso de forro Environmental thermal conditions and performance of broilers raised in aviaries with and without the use of polyethylene lining

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    P.G. Abreu

    2007-08-01

    Full Text Available O experimento foi realizado em delineamento inteiramente ao acaso, em esquema fatorial 2x2, para avaliar o efeito de época de ano, o tipo de forro de polietileno sobre as condições térmicas, de umidade e o desempenho de aves de corte. Foram utilizados quatro aviários, cada um dividido internamente em quatro boxes para alojar 200 aves cada. As variáveis de desempenho avaliadas foram peso vivo, ganho de peso, consumo de ração, conversão alimentar e mortalidade das aves do nascimento ao 42º dia de idade. O desempenho das aves foi melhor com aviários com forro. As condições térmicas foram melhoradas cm a utilização de forro nos aviários.This experiment was carried out in a completely randomized experimental design, factorial arrangement 2x2, to evaluate the effect of period of year and type polyethylene liming on thermal and humidity of aviaries and performance of broiler. Four aviaries were divided in four boxes to house 200 chickens each. The recorded performance traits were body weight, weight gain feed intake, feed:weght gain ratio and mortality from hatch to 42 days of age. Better thermal conditions and higher chicken performance were observed in the polyethylene liming aviaries.

  17. Parâmetros sangüíneos e urinários, no pré e pós parto, de búfalas criadas em sistema exclusivo de pastejo The blood and urinary values in the pre and pos-parturient period of buffaloes, kept on exclusive pasture feeding

    Directory of Open Access Journals (Sweden)

    Carlos Magno Chaves de Oliveira

    2003-06-01

    Full Text Available Foi avaliada a ocorrência de alterações nas concentrações de glicose sanguínea, proteína plasmática total, hematócrito e presença de corpos cetônicos na urina de oito búfalas leiteiras da raça Murrah, com idade variando entre 5 e 10 anos, com no mínimo duas lactações, clinicamente sadias, desde 60 dias antes até 60 dias pós-parto. As concentrações médias de glicose sangüínea e o valor médio do hematócrito diminuíram significativamente no pós-parto (pEight lactating buffaloes of the Murrah breed were used from 60 days before up to 60 days after calving, in order to evaluate alterations in the concentration of blood glucose, total plasmatic protein, haematocrit and the presence of ketonic bodies in the urine. The 5 to 10- year old buffaloes were clinically healthy and had had at least two lactations. During the experimental period the animals were maintained on Brachiaria brizantha pasture and supplemented with a mineral mixture and water.The media concentrations of blood glucose and the media values of the haematocrit diminished significantly after calving (p<0,05. The concentrations of total plasmatic protein did not show significant variation during the pre and post-calving period. The ketonic bodies in the pre-calving period were only detected in the urine of one buffaloe, but after the 32nd day of lactation ketonic bodies were detected in all animals. There was a direct relationship between the color of the urine positive by the Rothera test and blood glucose concentrations. It can be concluded that at the beginning of lactation the buffaloes had an energetic deficit, characterized by a decline of blood glucose concentrations and the presence of ketonic bodies in the urine, and that lactation caused a progressive decline of the hematocrit, but that the concentration of total plasmatic protein did not vary during the pre and post-calving period.

  18. Persistência na lactação para vacas da raça Holandesa criadas no Estado do Rio Grande do Sul via modelos de regressão aleatória Lactation persistency for Holstein cows raised in the State of Rio Grande do Sul using a random regression model

    Directory of Open Access Journals (Sweden)

    Cristian Kelen Pinto Dorneles

    2009-08-01

    Full Text Available Foram utilizados 21.702 registros de produção de leite no dia do controle de 2.429 vacas primíparas da raça Holandesa, filhas de 233 touros, coletados em 33 rebanhos do Estado do Rio Grande do Sul, entre 1992 e 2003, para estimar parâmetros genéticos, para três medidas de persistência (PS1, PS2 e PS3 e para a produção de leite até 305 dias (P305 de lactação. Os modelos de regressão aleatória ajustados aos controles leiteiros entre o sexto e o 300o dia de lactação incluíram o efeito de rebanho-ano-mês do controle, a idade da vaca ao parto e os parâmetros do polinômio de Legendre de ordem quatro, para modelar a curva média da produção de leite da população e os parâmetros do mesmo polinômio, para modelar os efeitos aleatórios genético-aditivo direto e de ambiente permanente. As estimativas de herdabilidade obtidas foram 0,05, 0,08 e 0,19, respectivamente, para PS1, PS2 e PS3 e 0,25, para P305 sugerindo a possibilidade de ganho genético por meio da seleção para PS3 e para P305. As correlações genéticas entre as três medidas de persistência e P305, variaram de -0,05 a 0,07, indicando serem persistência e produção, características determinadas por grupos de genes diferentes. Assim, consequentemente, a seleção para P305, geralmente praticada, não promove progresso genético para a persistência.There were used 21,702 test day milk yields from 2,429 first parity Holstein breed cows, daughters of 2,031 dams and 233 sires, distributed over 33 herds in the state of Rio Grande do Sul, from 1992 to 2003. Genetic parameters for three measures of lactation persistency (PS1, PS2 e PS3 and for milk production to 305 days (P305 were evaluated. A random regression model adjusted by fourth order Legendre polynomial was used. The random regression model adjusted to test day between the sixth and the 305th lactation day included the herd-year-season of the test day, the age of the cow at the parturition effects and the order fourth Legendre polinomial parameters, for modeling the milk production average curve of the population, and parameters of the same polinomial for modeling the random additive genetic and permanent environmental effects. The estimated heritabilities were 0.05, 0.08 and 0.19, respectively to PS1, PS2 and PS3, and 0.25 to P305, suggesting the possibility of a genetic gain by selection for PS3 and P305. The genetic correlations between persistency measurements and P305 ranged from -0.05 to 0.07, suggesting being, persistency and milk yield, characteristics determined by different gene groups, and that the selection for P305, usually done, do not promote genetic progress for persistency.

  19. Composição nutricional do casco da tartaruga-da-Amazônia (Podocnemis expansa criada em cativeiro e em idade de abate Centesimal nutrition of the tartaruga-da-Amazônia Podocnemis expansa shell bred in captivity and at slaughter age

    Directory of Open Access Journals (Sweden)

    Renata Cristina Scarlato

    2007-08-01

    Full Text Available A criação de tartarugas em cativeiro no Brasil vem se expandindo nos últimos anos, destacando-se o aumento do volume de abate da espécie Podocnemis expansa para obtenção de carne. Este estudo foi realizado na UFRuralRJ, objetivando-se analisar quantitativamente a composição centesimal, valor calórico, teor de colesterol, perfil em ácidos graxos e aminoácidos, e minerais de 60 cascos de P. expansa em idade de abate mantidas em criatório legalizado e registrado pelo IBAMA. Concluiu-se que o casco de P. expansa é rico em nutrientes, apresentando proteína de alto valor biológico para adultos, lipídios, ácidos graxos essenciais, minerais e baixo teor de colesterol, possibilitando sua utilização como suplemento nutricional após estudos futuros comprobatórios da biodisponibilidade dos nutrientes presentes.Breeding of turtles in Brazil has increased recently, and the increase of the volume of the species Podocnemis expansa to obtain meat is significant. This study was done at UFRuralRJ, and the aim was to analyse the centesimal composition, caloric value, tenor of cholesterol, fatty acids and amino acids and minerals of 60 P. expansa shells quantitativity at slaughter age maintained by IBAMA, a legal and registered breeder. It can be concluded that the P. expansa shell is rich in nutrients, with a high biological value protein for adults, lipids, essentials fatty acids, minerals and low tenor of cholesterol, possible to use as a nutritional supplement after corroborating future studies of the biodisponibility of the present nutrients.

  20. The role of botfly myiasis due to Dermatobia hominis L.Jr. (Diptera:Cuterebridae) as a predisposing factor to New World screwworm myiasis (Cochliomyia hominivorax coquerel) (Diptera:Calliphoridae).

    Science.gov (United States)

    Ruíz-Martínez, I; Gómez, F; Pérez, J M; Poudevigne, F A

    1996-07-23

    In the tropics, the botfly Dermatobia hominis and the NWS Cochliomyia hominivorax are the most important myiasis agents in cattle. It is frequently reported that furuncular lesions due to D. hominis are a predisposing cause for screwworm myiasis. Our results pointed out that only 5.2 to 7.4% of C. hominivorax gravid females oviposited in the offered furuncular lesions. Of 3242 eggs layed on botfly lesions (BFL), only 82 (2.5%) developed to second instar and died. In the flies tested, the furuncular lesions due to Dermatobia were used as food supply in 81.3% of the cases. In our opinion, the role of pH, the microflora associated with BFL, and the foruncular structure were the reasons for this lack of attraction. BFL do not serve as a predisposing factor for screwworm myiasis in the tropics.

  1. 不同组织源食物对大头金蝇生长发育的影响%Effect of Feeding on Different Tissues on Larva Development of Chrysomya megacephala (Diptera : Calliphoridae)

    Institute of Scientific and Technical Information of China (English)

    李学博; 吕宙; 万立华; 王尧; 姚岚; 王清山

    2012-01-01

    目的 观察猪的不同脏器组织来源食物对大头金蝇(Chrysomya megacephala)生长发育的影响.方法 在25℃恒温条件下,分别利用猪的脑、肝、肌肉,以及肌肉与脂肪的混合物(7:3)各200g饲养大头金蝇幼虫,约200只/组.幼虫孵化16h后,每12h测量幼虫的体长和体重,并测各组蛹长和蛹重,每次取样10只.观察记录各组不同阶段的样本数量,并计算发育历期,统计各实验组幼虫及蛹的死亡率和成虫的性别比,比较各组之间的差异.结果 肝组幼虫生长缓慢,与其他3组比较达到最大体长和体重的时间延迟36 h,其2龄和3龄幼虫期及总发育历期明显大于其他3组(P<0.01).混合食物组幼虫最大体长[(14.89±0.39)mm]显著小于脑组、肌肉组和肝组[分别为(17.81±0.54)、(16.94±0.43)和(17.14±0.27) mm] (P<0.01),肝组和混合食物组幼虫最大体重[分别为(73.5±6.8)和(63.0+5.4) mg]均显著小于脑组和肌肉组[分别为(91.2±7.5)和(86.3±7.3) mg] (P<0.01).混合食物组蛹长显著小于脑组、肌肉组和肝组(P<0.01),混合食物组和肝组蛹重均显著小于脑组和肌肉组(P<0.01).混合食物组幼虫和蛹的死亡率分别为(9.8±3.1)%和(8.9±3.1)%,显著高于脑组[(5.5±3.1)%和(4.6±1.5)%]、肌肉组[(4.7±2.2)%和(3.8±2.0)%]和肝组[(5.4±2.3)%和(4.8±1.7)%)](均P<0.01),后3组之间差异无统计学意义(P>0.05).各组成虫的性别比差异无统计学意义(P>0.05).结论 取食肝组织的大头金蝇幼虫发育历期较长,取食含有30%脂肪的混合食物的幼虫及蛹的体长和体重较小,死亡率亦较高.%Objective To observe the effect of feeding on different pig tissues on the development of Chrysomya megacephala larvae. Methods About 200 larvae each were reared on four different substrates, I.e. Pig's brain, liver, muscle and a mixture of minced pork muscle and fat (7 : 3) at a constant temperature of 25℃. Length and weight of larvae and pupae were measured at 12 h interval 16 h after eclosion. 10 larvae or pupae were collected each time. The time of development, mortality, and sex ratio of adults were recorded. Results Three replicated experiments showed that the larvae fed on liver grew slowly, time of reaching maximum length and weight was delayed for about 24-36 h, and the duration of larva development was longer than that of other groups (P0.05). Conclusion The development duration of the larvae fed on liver is longer than other groups. The body length and weight of larvae and pupae fed on mixture diet are less than other groups with higher mortality.

  2. 丝光绿蝇触角鞭节感受器官超显微形态研究%Ultrastructure of the antennal funiculus sensory organs of adult Lucilia sericata (Meigen) (Diptera:Calliphoridae)

    Institute of Scientific and Technical Information of China (English)

    刘贤慧; 李心钰; 张东

    2015-01-01

    Objectives] To investigate the ultrastructure, and discuss the potential functions of antennal sensilla on funiculus of Lucilia sericata, which is a synanthropic fly of great medical importance. [Methods] Using transmission electron microscopy and a laser scanning confocal microscope. [Results] The ultrastructure of four types of sensilla, including trichoid sensilla (Tr), basiconic sensilla (Ba), coeloconic sensilla (Co), and sensory sacculus (SS), were identified and descripted. [Conclusion] Tr and Ba may function as olfactory receptors, while Co might be involved in thermo- or hygro- reception. In the sensory sacculus, non-pore basiconic sensilla could be hygro- and thermo- sensilla, whereas basiconic-like sensilla and coeloconic-like sensilla might indicate a potential chemoreceptor function. Sensilla in the sacculus can function together, which demonstrates that the sensory sacculus acts as a functional complex. Due to the various types of sensilla and the complex sacculus, the antennae of flies could be a useful model for morphological, functional and evolutionary research on insect antennae.%【目的】观察研究重要的医学昆虫丝光绿蝇Lucilia sericata触角感受器的形态,以明确不同类型感受器的结构及功能。【方法】采用透射电镜与激光共聚焦显微镜技术相结合的方法。【结果】明确并详细描述了毛型感受器、锥型感受器、腔锥型感受器及感觉囊的形态结构。【结论】毛型感受器和锥型感受器可能为化学感受器,腔锥型感受器可能为温湿度感受器;感觉囊中的无孔锥型感受器可能为温湿度感受器,类锥型感受器及类腔锥型感受器可能为化学感受器,各类型感受器同时行使功能,表明感觉囊为一个功能复合体。蝇类触角的感器类型多样、囊结构复杂,可作为研究昆虫触角感器形态、功能及演化的模式类群。

  3. Morphology and identification of first instars of the European and Mediterranean blowflies of forensic importance

    DEFF Research Database (Denmark)

    Szpila, K.; Hall, M. J. R.; Pape, T.

    2013-01-01

    First instars of Lucilia ampullacea Villeneuve, Lucilia caesar Linnaeus, Lucilia cuprina Weidemann, Lucilia richardsi Collin, Lucilia sericata Meigen and Lucilia silvarum Meigen (Diptera: Calliphoridae) are thoroughly documented with scanning electron microscopy images, light microscopy photographs...

  4. Preliminary evaluation of maggot (Diptera: Calliphoridae) therapy as a potential treatment for leishmaniasis ulcers Evaluación preliminar en un modelo animal de la terapia con larvas de Lucilia sericata para el tratamiento de la leishmaniasis cutánea

    OpenAIRE

    Jazzmin Arrivillaga; Milagros Oviedo; Jaime Rodríguez

    2008-01-01

    Introduction. Maggot debridement therapy has been widely used for treating a variety of scarred-over soft-tissue wounds. Published accounts record several illnesses in which treatment with larval therapy has promoted injury healing in conjunction with infection by bacterial pathogens resistant to conventional antibiotics.
    Objective. An initial test of the maggot therapy was developed for cutaneous injuries produced by Leishmania amazonensis.
    Materials and methods. An exp...

  5. 家蝇和大头金蝇在麦麸和猪瘦肉上的产卵选择和发育差异%Difference in oviposition preference and development between Musca Domestica (Diptera:Muscidae)and Chrysomya megacephala (Diptera:Calliphoridae) on wheat bran and pork muscle

    Institute of Scientific and Technical Information of China (English)

    王争艳; 莫建初

    2009-01-01

    In many areas in China, Chrysomya megacephala F. Has gradually invaded the ecological niches occupied by Musca domestica L. And become the dominant species at garbage sites in the urban areas. To explore the influence of foods on the development of M. Domestica and C. Megacephala larvae when theywere reared solely or jointly, the oviposition preference and life history of the F, generation of wild blowfly C. Megacephala and housefly M. Domestica on wet wheat bran, pork muscle and their mixture were observed in the laboratory. The results showed that: (1) Blowflies preferred to lay their eggs on substrates containing pork muscle while houseflies preferred to lay their eggs on substrates containing wheat bran. (2) Newly-hatched housefly and blowfly larvae could reach adulthood on diets containing pork muscle. When raised on wheat bran, newly-hatched housefly larvae could reach adulthood while newly-hatched and 2nd instar blowfly larvae all died before entering into the next instar, but some 3rd instar blowfly larvae could reach adulthood. (3) When the same number of newly-hatched housefly and blowfly larvae grew mixedly on diets containing pork muscle, blowflies exhibited shorter developmental duration and higher survival rate than houseflies. When reared mixedly with houseflies on wheat bran, blowflies showed shorter developmental duration and higher survival rate than the same instar blowflies that grew solely on wheat bran. These results suggest that in coexistence the housefly can promote the utilization of the vegetable resource by the blowfly. This might be one of important reasons why the blowfly can invade successfully the ecological niches occupied dominantly by the housefly.%在中国许多地区,大头金蝇 Chrysomya megacephala F.已侵入过去由家蝇Musca domestica L.占绝对优势的垃圾生态位,逐渐成为城市蝇类的优势种.为了解单独和混合饲养时食物种类对家蝇和大头金蝇幼虫生长发育的影响,在室内观察了野外大头金蝇和家蝇F1,代在湿麦麸、猪瘦肉以及两者混合物上的产卵选择和生活史.结果显示:(1)大头金蝇嗜在含有猪瘦肉的基质上产卵,而家蝇嗜在含有麦麸的基质上产卵;(2)初孵家蝇和大头金蝇幼虫都能在含有瘦肉的食物上发育至成虫.在麦麸上,初孵家蝇幼虫能发育至成虫,而初孵和2龄大头金蝇幼虫在进入下一龄期前全部死亡,但少数3龄大头金蝇幼虫能发育至成虫;(3)等量初孵家蝇与大头金蝇在含有猪瘦肉的食物上共同生长时,与家蝇相比,大头金蝇的发育历期较短、存活率较高.与家蝇在麦麸上共同生长时,与在麦麸上独立生长的同龄大头金蝇相比,大头金蝇的发育历期较短、存活率较高.这些结果表明,共生时家蝇可促进大头金蝇对植物质营养的利用,这也许是大头金蝇能成功侵入家蝇占绝对优势的垃圾生态位的一个重要原因.

  6. Comparsion of the efficacy of chemomechanical and mechanical methods of caries removal in the reduction of streptococcus mutans and lactobacillus spp in carious dentine of primary teeth Comparação da eficácia dos métodos químico-mecânico e mecânico de remoção de cárie na redução de streptococcus mutans e lactobacillus spp da dentina criada de dentes decíduos

    Directory of Open Access Journals (Sweden)

    Gisele Quariguasi Tobias Lima

    2005-12-01

    Full Text Available The methods of caries removal have been the subject of many studies over the last years. At present, methods involving the least tissue invasion have been outstanding in the field of surgical-restorative treatment. In this context, the Carisolv™ system has appeared as a less traumatic and less invasive approach, particularly in pedodontics. Thus, the objective of the present study was to carry out a comparative analysis of the dentinal structure of primary molars before and after the removal of carious tissue by mechanical (low speed drills and conventional dentinal curettes and chemomechanical (Carisolv™ kit procedures based on quantitative culture for cariogenic bacteria to determine the number of bacteria present in the carious dentine after both treatments. Sixty primary molars from children ranging in age from 4 to 8 years, with active occlusal caries in dentine, were divided into two groups (A and B of 30 teeth each, with group A having been treated by the chemomechanical technique and group B by the mechanical technique. Dentin samples were placed in glass vials containing 1 mL thioglycolate broth and submitted to culture to determine the number of S. mutans and Lactobacillus per mg of decayed dentine. The results did not reveal significant differences between the two methods of caries removal; however, the chemomechanical method was more efficient in completely eliminating S. mutans (p=0.02. In summary, the present results confirm previous studies showing that the two methods are comparable in reducing Lactobacillus, but Carisolv™ is more effective in the elimination of S. mutans.Os métodos de remoção de cárie têm sido objeto de muitos estudos nos últimos anos. Atualmente, aqueles que determinam mínima invasão tecidual têm se sobressaído no campo do tratamento cirúrgico-restaurador. Neste contexto, surgiu o sistema Carisolv™ como uma abordagem menos traumática, com destaque na odontopediatria e menos invasiva. Desta forma, este estudo teve como objetivo analisar comparativamente a estrutura dentinária de molares decíduos antes e após a remoção do tecido cariado pelos métodos mecânico (brocas de baixa rotação e curetas dentinárias convencionais e químico-mecânico (Kit Carisolv™, através de exame microbiológico quantitativo, verificando-se o número de bactérias/mg presentes na dentina cariada após cada tratamento. Foram utilizados 60 molares decíduos de crianças na faixa etária de 4 a 8 anos de idade que apresentavam cárie oclusal ativa em dentina, os quais foram divididos em dois grupos (A e B de 30 dentes cada, onde o grupo A foi tratado com a técnica químico-mecânica e o grupo B foi tratado com a técnica mecânica. As amostras de dentina foram coletadas e depositadas em frascos de vidro contendo 1mL de caldo Tioglicolato e pérolas de vidro. Em seguida, foram imediatamente encaminhadas para análise microbiológica para que fossem determinadas as concentrações de S. mutans e Lactobacillus por mg de dentina cariada. Os resultados não revelaram diferenças significantes na comparação dos dois métodos de remoção de cárie; contudo, foi verificado que o método químico-mecânico era mais eficiente na eliminação total de S. mutans (p=0,02. Em síntese, os nossos resultados confirmam alguns estudos prévios em que os métodos são comparáveis na redução de Lactobacillus, mas o Carisolv™ é mais eficaz na eliminação de S. mutans.

  7. Comparsion of the efficacy of chemomechanical and mechanical methods of caries removal in the reduction of streptococcus mutans and lactobacillus spp in carious dentine of primary teeth Comparação da eficácia dos métodos químico-mecânico e mecânico de remoção de cárie na redução de streptococcus mutans e lactobacillus spp da dentina criada de dentes decíduos

    OpenAIRE

    Gisele Quariguasi Tobias Lima; Eurípedes Gomes de Oliveira; João Inácio Lima de Souza; Valério Monteiro Neto

    2005-01-01

    The methods of caries removal have been the subject of many studies over the last years. At present, methods involving the least tissue invasion have been outstanding in the field of surgical-restorative treatment. In this context, the Carisolv™ system has appeared as a less traumatic and less invasive approach, particularly in pedodontics. Thus, the objective of the present study was to carry out a comparative analysis of the dentinal structure of primary molars before and after the re...

  8. Sarcosaprophagous Diptera assemblages in natural habitats in central Spain: spatial and seasonal changes in composition.

    Science.gov (United States)

    Martín-Vega, D; Baz, A

    2013-03-01

    The composition and spatial distribution of sarcosaprophagous Diptera assemblages were studied using carrion-baited traps along a bioclimatic gradient of natural habitats in central Spain throughout the different seasons during 1 year. Calliphoridae and Muscidae were the most abundant families, accounting for, respectively, 41.9% and 35.1% of all Diptera specimens collected. Other abundant families were Heleomyzidae (8.4%), Sarcophagidae (6.9%) and Piophilidae (5.1%). Fly assemblage compositions differed among bioclimatic levels, with Chrysomya albiceps (Wiedemann) (Diptera: Calliphoridae) being the dominant species in mesomediterranean habitats, Muscina levida (Harris) (Diptera: Muscidae) the dominant species in supramediterranean habitats, and Prochyliza nigrimana (Meigen) (Diptera: Piophilidae) the dominant species in oromediterranean habitats. Differences in assemblage composition were also found among seasons. Thermophobic species such as Calliphora vicina Robineau-Desvoidy (Diptera: Calliphoridae) and some species of Heleomyzidae were well represented during autumn, winter and spring in the three bioclimatic levels sampled. By contrast, thermophilic species such as Ch. albiceps and Lucilia sericata (Meigen) (Diptera: Calliphoridae) and most Muscidae and Sarcophagidae species were more abundant during summer and in mesomediterranean habitats located at lower elevations. Knowledge of the preferences of some species for certain habitats may be of ecological and forensic value and may establish a starting point for further research.

  9. Characteristics of African-American literature in Kathryn Stockett´s The Help and Alice Walker´s The Color Purple

    OpenAIRE

    Greve, Nina Louise

    2016-01-01

    Discuto si la novela Señoras y Criadas, escrita por una norteamericana blanca, puede considerarse una novela afroamericana a pesar de la etnicidad de la autora. La novela trata sobre la vida de las criadas afroamericanas en los Estados Unidos en la época de los 60, y se ha comparado con El Color Púrpura por su enfoque en el sufrimiento de la mujer negra. En este trabajo, primero establezco cuáles son los elementos esenciales de la literatura afroamericana: la identidad, el rol de la mujer y ...

  10. Characteristics of African-American literature in Kathryn Stockett´s The Help and Alice Walker´s The Color Purple

    OpenAIRE

    Greve, Nina Louise

    2016-01-01

    Discuto si la novela Señoras y Criadas, escrita por una norteamericana blanca, puede considerarse una novela afroamericana a pesar de la etnicidad de la autora. La novela trata sobre la vida de las criadas afroamericanas en los Estados Unidos en la época de los 60, y se ha comparado con El Color Púrpura por su enfoque en el sufrimiento de la mujer negra. En este trabajo, primero establezco cuáles son los elementos esenciales de la literatura afroamericana: la identidad, el rol de la mujer y ...

  11. Rate of development of forensically-important Dipterain southern Brazil

    Directory of Open Access Journals (Sweden)

    Rodrigo F. Krüger

    2010-01-01

    Full Text Available Dipteran larvae were collected from rabbit (Oryctolagus cunniculus L. carcasses during the four seasons in 2005 in the southernmost state of Rio Grande do Sul, Brazil. The larvae were fed ground beef at ambient temperatures following collection from carcasses. The development of each species under these conditions was estimated. The most abundant species in the carcasses were Lucilia eximia (Wiedemann and Chrysomya albiceps (Wiedemann (Calliphoridae, and they were found in all seasons. The data were fitted to a linear model that describes the relationship between temperature and linear developmental rating. These two species are primary forensic indicators in southern Brazil. Other species such as Hemilucilia semidiaphana (Rondani (Calliphoridae, Synthesiomyia nudiseta (Wulp, Muscina stabulans (Fallen (Muscidae, and Fannia pusio (Wiedemann (Fanniidae were forensically less important because they only occurred in high frequency in certain seasons and during the first days of carcass decomposition.

  12. Necrophagous Muscoids that develop in carcasses of Didelphis albiventris Lund, 1841 (Didelphimorphia, Didelphidae in Rio Grande do Sul, Brazil

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    Ândrio Zafalon da Silva

    2010-06-01

    Full Text Available Information regarding the similarity of the fauna that colonizes decomposing wildlife is insipient among the different carcass models. Species with different tissues and alimentary diets are hypothesized to be a colonization source of different necrophagous species. To verify this hypothesis, we observed the decomposition of a carcass of Didelphis albiventris Lund, 1841 (Didelphimorphia, Didelphidae, that had been run over by a vehicle. 2,273 adults were reared from specimens at immature stages collected on the carcass. The most representative species belongs to Calliphoridae, Muscidae, Sarcophagidae and Fanniidae, with numeric dominance of Lucilia eximia Wiedemann, 1819 (Diptera, Calliphoridae. Another six species were found, besides non-identified Fanniidae specimens. All species were colonizers of traditionally used forensic models.

  13. Comparative fly species composition on indoor and outdoor forensic cases in Malaysia.

    Science.gov (United States)

    Syamsa, Rizal Abdullah; Omar, Baharudin; Ahmad, Firdaus Mohd Salleh; Hidayatulfathi, Othman; Shahrom, Abd Wahid

    2017-01-01

    Forensic entomology refers to the science of collection and analysis of insect evidence in order to determine the minimum time period since death. This study aimed to investigate the occurrence of forensically important flies on 34 human remains referred to Universiti Kebangsaan Malaysia Medical Centre over a period of three years. Entomological specimens were collected at the death scenes and/or during autopsies. Live specimens were reared into adults while preserved specimens were processed for species identification. Five families, seven genera and nine species of flies were identified from human remains. The results of the study showed Chrysomya megacephala (Calliphoridae) maggots occurred on corpses with the highest frequency (70.6%), followed by Ch. rufifacies (Calliphoridae) (44.1%), sarcophagid fly (Sarcophagidae) (38.2%), Synthesiomya nudiseta (Muscidae) (20.6%), Megaselia scalaris (Phoridae) (14.7%), Lucilia cuprina (Calliphoridae) (5.9%), Ch. nigripes (Calliphoridae) (5.9%), Eristalis spp. (Syrphidae) (5.9%) and Hydrotaea spinigera (Muscidae) (2.9%). The greatest fly diversity occurred on remains recovered indoors (eight species) compared to outdoors (three species). Whilst, single and double infestations were common for both indoor and outdoor cases, multiple infestation of up to six species was observed in one of the indoor cases. Although large numbers of fly species were found on human remains, the predominant species were still those of Chrysomya, while S. nudiseta was found only on human remains recovered from indoors. The present study provides additional knowledge in the context of Malaysian forensic entomology and the distribution of forensically important flies which is of relevance to forensic science. Copyright © 2016 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.

  14. A Preliminary Analysis of Insects of Medico-legal Importance in Curitiba, State of Paraná

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    Mauricio Osvaldo Moura

    1997-03-01

    Full Text Available A survey of the carrion fauna was made at two sites in Curitiba, State of Paraná, with the objective of describing the insects associated with carrion and setting up a preliminary data-base for medico-legal purposes in south Brazil. Vertebrate exclusion experiments were carried out in each season between 1994 and 1995 with a 250 g laboratory-bred rat (Rattus norvegicus. Five stages of decomposition were identified: fresh, bloated, decaying, dry and adipocere-like. Some species showed seasonal and site preference and so could be used to identify the probable place and season where death took place. Sarconesia chlorogaster (Diptera, Calliphoridae was restricted to an open field site and to cooler months. Hemilucilia semidiaphana (Diptera, Calliphoridae and Pattonella resona (Diptera, Sarcophagidae were restricted to the forest site and warmer months. Phaenicia eximia (Diptera, Calliphoridae and Oxyletrum discicolle (Coleoptera, Silphidae were present at both sites throughout the year and could be useful for population level analysis. Dissochaetus murray (Coleoptera, Cholevidae was present throughout the year at the forest site and was associated with the adipocere-like stage. Ants played an important role producing post-mortem injuries to the carcasses. Insects of 32 species are reported as being useful in community level approaches

  15. A checklist of arthropods associated with pig carrion and human corpses in Southeastern Brazil

    Directory of Open Access Journals (Sweden)

    LML Carvalho

    2000-01-01

    Full Text Available Necrophagous insects, mainly Diptera and Coleoptera, are attracted to specific stages of carcass decomposition, in a process of faunistic succession. They are very important in estimating the postmortem interval, the time interval between the death and the discovery of the body. In studies done with pig carcasses exposed to natural conditions in an urban forest (Santa Genebra Reservation, located in Campinas, State of São Paulo, southeastern Brazil, 4 out of 36 families of insects collected - Calliphoridae, Sarcophagidae, Muscidae (Diptera and Dermestidae (Coleoptera - were considered of forensic importance, because several species were collected in large numbers both visiting and breeding in pig carcasses. Several species were also observed and collected on human corpses at the Institute of Legal Medicine. The species belonged to 17 different families, 6 being of forensic importance because they were reared from human corpses or pig carcasses: Calliphoridae, Sarcophagidae, Muscidae, Piophilidae (Diptera, Dermestidae, Silphidae and Cleridae (Coleoptera. The most important species were: Diptera - Chrysomya albiceps, Chrysomya putoria, Hemilucilia segmentaria, Hemilucilia semidiaphana (Calliphoridae, Pattonella intermutans (Sarcophagidae, Ophyra chalcogaster (Muscidae, Piophila casei (Piophilidae; Coleoptera - Dermestes maculatus (Dermestidae, Oxyletrum disciolle (Silphidae and Necrobia rufipes (Cleridae.

  16. Effect of bait decomposition on the attractiveness to species of Diptera of veterinary and forensic importance in a rainforest fragment in Brazil.

    Science.gov (United States)

    Oliveira, Diego L; Soares, Thiago F; Vasconcelos, Simão D

    2016-01-01

    Insects associated with carrion can have parasitological importance as vectors of several pathogens and causal agents of myiasis to men and to domestic and wild animals. We tested the attractiveness of animal baits (chicken liver) at different stages of decomposition to necrophagous species of Diptera (Calliphoridae, Fanniidae, Muscidae, Phoridae and Sarcophagidae) in a rainforest fragment in Brazil. Five types of bait were used: fresh and decomposed at room temperature (26 °C) for 24, 48, 72 and 96 h. A positive correlation was detected between the time of decomposition and the abundance of Calliphoridae and Muscidae, whilst the abundance of adults of Phoridae decreased with the time of decomposition. Ten species of calliphorids were registered, of which Chrysomya albiceps, Chrysomya megacephala and Chloroprocta idioidea showed a positive significant correlation between abundance and decomposition. Specimens of Sarcophagidae and Fanniidae did not discriminate between fresh and highly decomposed baits. A strong female bias was registered for all species of Calliphoridae irrespective of the type of bait. The results reinforce the feasibility of using animal tissues as attractants to a wide diversity of dipterans of medical, parasitological and forensic importance in short-term surveys, especially using baits at intermediate stages of decomposition.

  17. A idéia de progresso no processo de institucinalização nacional das ciências no Brasil: a sociedade auxiliadora da indústria nacional

    Directory of Open Access Journals (Sweden)

    Bertol Domingues, Heloisa María

    1996-12-01

    Full Text Available The first Scientific institutions in Brasil created during the colonial period, defended the value of the thought that identified Enlightenment with Science. We would like to point out the roll played in the construction of the Nacional State by the Sociedade Auxiliadora da Industria Nacional, created after the independency, in 1825, although working officially since 1827.

    [pt] No Brasil, as primeiras instituções científicas que foram criadas ainda no tempo colonial, foram sociedades científicas, que professavam os valores daquele pensamento que identificava as luzes com as ciências. Gostaria de destacar aqui o Auxiliadora da Indusria Nacional, criada logo após a independê, em 1825 e funcionando oficialmente desde 1827.

  18. Unidades de conservação, Legislação ambiental e a APA Petrópolis

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    Julieta Laudelina de Paiva

    2010-12-01

    Full Text Available As unidades de conservação são criadas com o objetivo de manter a diversidade biológica, proteger espécies ameaçadas de extinção, preservar e restaurar ecossistemas naturais. A Área de Proteção Ambiental da Região Serrana de Petrópolis – APA PETRÓPOLIS, primeira APA federal, foi criada por apresentar rica biodiversidade além de ter seus atributos naturais relativamente bem conservados. A população residente na APA Petrópolis está submetida ao cumprimento de várias determinações legais nos três níveis administrativos.

  19. Siete especies nuevas de Allorhogas (Hymenoptera: Braconidae: Doryctinae) de México

    OpenAIRE

    Juan José Martínez; Alejandro Zaldívar-Riverón

    2015-01-01

    Se describen, ilustran y caracterizan molecularmente 7 especies nuevas del género gallícola Allorhogas (Braconidae: Doryctinae): A. amuzgo sp. nov., A. coccolobae sp. nov., A. crassifemur sp. nov., A. jaliscoensis sp. nov., A. marshi sp. nov., A. parvus sp. nov. y A. scotti sp. nov. Estas especies fueron recolectadas en bosques tropicales caducifolios de la costa del Pacífico mexicano en Jalisco, Oaxaca y Guerrero. Allorhogas coccolobae fue criada de agallas foliares en Coccoloba barbadensis ...

  20. The theory of social representations in environmental studies

    OpenAIRE

    Fagundes, Beatriz

    2009-01-01

    This article has for finality to present a reflection about the Theory of Social Representations, created by Moscovici in 1961, up from its historical context after looking for to understand like ordinary people build social representations about certain phenomenon and how they contribute to the geographic studies. Este artigo tem por finalidade apresentar uma reflexão sobre a Teoria das Representações Sociais, criada por Moscovici em 1961, partindo do seu contexto histórico, depois procu...

  1. Fenomenologia e Existencialismo: articulando nexos, costurando sentidos

    Directory of Open Access Journals (Sweden)

    Ariane Patrícia Ewald

    2014-04-01

    Full Text Available O presente texto procura expor historicamente o surgimento do movimento fenomenológico e do existencialismo, explorando alguns dos seus principais expoentes e algumas noções fundamentais. Procura também discutir a dificuldade em definir um termo que dê conta da variedade de concepções criadas a partir de cada autor, tanto na fenomenologia quanto no existencialismo.

  2. A vanguarda e a dialéctica: uma nota sobre a nouvelle vague

    Directory of Open Access Journals (Sweden)

    Luís Miguel Cardoso

    2016-02-01

    Na caminhada que afastava o romance das formulações tradicionais do enredo, surgia, como nos diz Aguiar e Silva2, o Nouveau Roman, designação criada por jornalistas, que identificava uma tipologia que aparecera após 1950, possuindo como principal ideal o afastamento dos vectores tradicionais na concepção do romance e uma aproximação a Joyce, Woolf, Faulkner e Dos Passos.

  3. Inventario de los parasitoides de Liriomyza spp. (Diptera: Agromyzidae) en la región sur de Honduras

    OpenAIRE

    2016-01-01

    Se presenta un inventario de 25 especies de parasitoides (Hymenoptera) que atacan a tres especies de minadores foliares (Liriomyza) en tres departamentos de la región sur de Honduras. La especie predominante fue Liriomyza sativae. criada de 25 especies de cultivos y plantas silvestres. Las infestaciones de L. sativae fueron mayores en cultivos de Cucurbitaceae, Solanaceae y Fabaceae, y en las plantas silvestres Kallstroemia maxima (Zygophyllaceae) y Ricinus communis (Euphorbiaceae). Se detall...

  4. Experiência de repovoamento com Sicalis flaveola brasiliensis (Gmelin, 1789 (Passeriformes, Emberizidae em área destinada à pecuária leiteira

    Directory of Open Access Journals (Sweden)

    Luiz Octavio Marcondes-Machado

    1988-01-01

    Full Text Available Relatam-se experiências feitas com exemplares de Sicalis flaveola brasiliensis, reintroduzidos, a partir de aves criadas em cativeiro e também recentemente capturadas, para repovoar a fazenda Jatibaia, em Campinas, Estado de São Paulo.Experiments made with Sicalis flaveola brasiliensis are related. Specimens both bred in captivity and recently captured were realeased to recolonize the farm Jatibaia, at Campinas, state of São Paulo, Brazil.

  5. Experiência de repovoamento com Sicalis flaveola brasiliensis (Gmelin, 1789) (Passeriformes, Emberizidae) em área destinada à pecuária leiteira

    OpenAIRE

    Luiz Octavio Marcondes-Machado

    1988-01-01

    Relatam-se experiências feitas com exemplares de Sicalis flaveola brasiliensis, reintroduzidos, a partir de aves criadas em cativeiro e também recentemente capturadas, para repovoar a fazenda Jatibaia, em Campinas, Estado de São Paulo.Experiments made with Sicalis flaveola brasiliensis are related. Specimens both bred in captivity and recently captured were realeased to recolonize the farm Jatibaia, at Campinas, state of São Paulo, Brazil.

  6. Monitoramento das helmintoses gastrintestinais em rebanho leiteiro criado em sistema de produção orgânica na Fazendinha Agroecológica.

    OpenAIRE

    2009-01-01

    O objetivo deste trabalho foi utilizar técnicas parasitológicas para monitorar o grau de parasitismo como subsídio para a criação de programas de controle das infecções por nematóides gastrintestinais em vacas leiteiras criadas em sistema orgânico na Fazendinha Agroecológica, convênio Embrapa/Universidade Federal Rural do Rio de Janeiro (UFRuralRJ)/Pesagro-Rio.

  7. Patient with Tracheostomy Parasitized in Hospital by Larvae of the Screwworm, Cochliomyia hominivorax

    Science.gov (United States)

    Batista-da-Silva, José A.; Borja, Gonzalo E. M.; Queiroz, Margareth M. C.

    2011-01-01

    Myiasis is the infestation of living vertebrates by fly larvae that feed for at least part of their development on the host's dead or living tissues, body substances, or ingested food. The occurrences of traumatic myiasis in humans and animals in urban and rural environments represent serious economic and public health concerns. This study reports a 49-year-old tracheostomized man undergoing chemotherapy treatment who was parasitized in the hospital in São Gonçalo, Rio de Janeiro, Brazil, by larvae of the screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) in the thoracic cavity. PMID:22243525

  8. Records of Chrysomya albiceps in Northern Italy: an ecological and forensic perspective

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    S Vanin

    2009-07-01

    Full Text Available Knowledge of the carrion-breeding insects present at a local level is important and necessary for defining the post-mortem interval. Climate changes and globalisation are affecting species ranges and population dynamics. In this note, we report the incidence of Chrysomya albiceps (Diptera: Calliphoridae on dead human bodies and carrion in Northern Italy. These data confirm the spread of this species in the Northern regions. The partial sequencing of a 583-bp region of the cytochrome oxidase subunit 1 gene of an Adriatic population did not reveal any difference compared to the same genomic region in the African and South American populations of this species.

  9. Sex-biased captures of sarcosaprophagous Diptera in carrion-baited traps.

    Science.gov (United States)

    Martín-Vega, Daniel; Baz, Arturo

    2013-01-01

    The use of carrion-baited traps is a common and widely extended practice in the study of sarcosaprophagous Diptera. However, it implies different areas of bias, one of them being the different responses of males and females to carrion bait, which results in possible biased sex ratios in the captures. In the present study, the use of carrion-baited traps revealed significant female-biased captures in the families Calliphoridae, Muscidae, and Sarcophagidae, whereas the collected species of the families Piophilidae, Heleomyzidae, and Ulidiidae showed different patterns in the observed sex ratios. Possible explanations according to existing literature and the types of mating behaviors of the different families are discussed.

  10. Calculating time since death in a mock crime case comparing a new computational method (ExLAC) with the ADH method.

    Science.gov (United States)

    Reibe-Pal, Saskia; Madea, Burkhard

    2015-03-01

    We compared the results of calculating a minimum post-mortem interval (PMImin) in a mock crime case using two different methods: accumulated degree hours (ADH method) and a newly developed computational model called ExLAC. For the ADH method we further applied five reference datasets for the development time of Calliphora vicina (Diptera: Calliphoridae) from 5 different countries and our results confirmed the following: (1) Reference data for blowfly development that has not been sampled using a local blowfly colony should not, in most circumstances, be used in estimating a PMI in real cases; and (2) The new method ExLAC might be a potential alternative to the ADH method.

  11. Determination of methylphenidate in Calliphorid larvae by liquid-liquid extraction and liquid chromatography mass spectrometry - Forensic entomotoxicology using an in vivo rat brain model

    DEFF Research Database (Denmark)

    Bushby, Sarah K.; Thomas, Nicky; Priemel, Petra A.;

    2012-01-01

    and Calliphorid larvae) by liquid-liquid extraction with recovery of >80%, and quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LC-MS/MS assay was validated for entomotoxicological use and initially applied to male Sprague-Dawley rats (n=6) that were dosed with MPH (20mg/kg) ante......The aim of this study was to examine the potential forensic utilisation of blowfly larvae (Diptera: Calliphoridae) as an alternative toxicological specimen for the detection of the psychotropic model drug methylphenidate (MPH). MPH was extracted from biological matrices (rat brain, serum...

  12. Foraging by Polybia (Trichothorax ignobilis (Hymenoptera, Vespidae on flies at animal carcasses Forrageamento de Polybia (Trichothorax ignobilis sobre moscas visitantes de carcaças animais

    Directory of Open Access Journals (Sweden)

    Leonardo Gomes

    2007-09-01

    Full Text Available Proteins for brood nutrition of social wasps are obtained from many prey, including insects (even bees and other wasps, spiders and bits of decaying meat. After being captured and killed, prey are reduced to a shapeless mass and distributed to the brood. Little is known about the foraging activity, especially on this group. Herein we describe the sequence of foraging behaviours of the social wasp Polybia (Trichothorax ignobilis for hunting flies (Diptera: Calliphoridae and Muscidae over pig carcasses. To our knowledge, there are few scientific descriptions of prey foraging behaviour on this species.Proteínas para nutrição da prole de vespas sociais são obtidas de várias presas, incluindo insetos (mesmo abelhas e outras vespas, aranhas e pedaços de carne em decomposição. Após serem capturadas e mortas, as presas são reduzidas a massas amorfas e distribuídas para a prole. Pouco é sabido sobre a atividade de forrageamento, especialmente nesse grupo. Assim nós descrevemos a seqüência de comportamento de forrageamento da vespa social Polybia (Trichothorax ignobilis capturando moscas (Díptera: Calliphoridae e Muscidae em carcaças de porcos. Para nosso conhecimento, há poucas descrições científicas do comportamento de forrageamento nessa espécie.

  13. An initial study of insect succession on decomposing rabbit carrions in Harare, Zimbabwe

    Institute of Scientific and Technical Information of China (English)

    Nyasha Mabika; Ron Masendu; Gilbert Mawera

    2014-01-01

    Objective: To investigate insects visiting sun exposed and shaded decomposing rabbit carcasses and to establish the relationship between insects and carcasses which may be of forensic importance in Harare. Methods: Two rabbits weighing 2.3 kg and 2.5 kg were killed by sharp blows on the head. One was exposed to the sun while the other was placed under shade. The carcasses were allowed to decompose and insects were collected twice a day for the first week and thereafter once a day up to the end of the 7 weeks. Maggots were also collected from the decomposing carcasses and reared.Results:Five dipteran families (Calliphoridae, Muscidae, Sarcophagidae, Phoridae and Drosophilidae) were identified from the sun-exposed carcass. Species collected included Luciliacuprina (L. cuprina), Chrysomya albiceps (C. albiceps), Musca domestica, Sarcophaga sp. and Drosophila sp. Four families (Calliphoridae, Muscidae, Phoridae, Anthomyiidae) were identified from the shaded carcass. Representatives of these families included L. cuprina, C. albiceps, Musca domestica, and Hydrotaea sp. Three Coleopteran families (Histeridae, Cleridae and Dermestidae) were identified from both carcasses. The observed species were Saprinus sp., Necrobia rufipes and Dermestes sp. Formicidae (Hymenoptera) was represented by only one species (Pheidole sp.). Flies which emerged from the rearing units were L. cuprina, Lucilia sp., C. albiceps, Sarcophaga sp. and Sepsis sp.). Conclusions: Of the dipteran species collected during the study, L. cuprina and C. albiceps could be important for further forensic studies since they were collected from the carcasses and also observed from the rearing units.

  14. Insects (Diptera) associated with cadavers at the Institute of Legal Medicine in Pernambuco, Brazil: implications for forensic entomology.

    Science.gov (United States)

    Oliveira, Tatiana Costa; Vasconcelos, Simao Dias

    2010-05-20

    Increasing rates of unsolved homicides in Brazil prompt the need for applied entomological data to be used as a complementary tool by criminal investigators. In that context, we analyzed the occurrence of forensically important insect species (Order Diptera) on 14 cadavers taken into the Institute of Legal Medicine (ILM), in Pernambuco, Brazil, according to the conditions of the body and the pattern of colonisation by insects. Simultaneously, we surveyed the diversity of insects in the surrounding environment using bait traps. Five species were present on cadavers: Chrysomya albiceps, Chrysomya megacephala and Cochliomyia macellaria (Calliphoridae), Oxysarcodexia riograndensis and Ravinia belforti (Sarcophagidae). A total of 4689 adult insects belonging to 24 species of seven dipteran families (Calliphoridae, Sarcophagidae, Muscidae, Fanniidae, Phoridae, Anthomyiidae and Stratiomyidae) was collected at the ILM premises. C. albiceps was the most frequent species on the corpses and the most abundant in the traps. Species referred to as of forensic importance, such as Lucilia eximia, Chrysomya putoria, Oxysarcodexia modesta and Ophyra chalcogaster were collected on traps, but not on cadavers. There seems to be a limited colonisation of cadavers at the scene of the death, despite the ubiquity of necrophagous species in the area. The results contribute to differentiate between species that are involved in decomposition and those found in and around the mortuary installations of the ILM, thus providing potential clues about the locality of death and the post-mortem interval.

  15. Forensic entomology of high-rise buildings in Malaysia: Three case reports.

    Science.gov (United States)

    Syamsa, R A; Omar, B; Zuha, R M; Faridah, M N; Swarhib, M S; Hidayatulfathi, O; Shahrom, A W

    2015-06-01

    The distributions of flies are not only confined to ground level but can also be at higher altitudes. Here, we report three forensic cases involving dipterans in high-rise buildings in Kuala Lumpur, Malaysia. Case 1 involved a corpse of adult female found at the top floor of a fifteen-story apartment. Case 2 dealt with a body of a 75-year-old female discovered in a bedroom on the eleventh floor of an eighteen-story building, while Case 3 was a 52-year-old male found in his fifth floor shop house. Interestingly, entomological analysis revealed that all corpses were infested with similar Dipterans: Megaselia scalaris (Loew) (Diptera: Phoridae), Synthesiomyia nudiseta (Wulp) (Diptera: Muscidae) and sarcophagid (Diptera: Sarcophagidae). The first two species were commonly associated with corpses found indoors at ground level. We noted the additional occurrence of blowflies Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) and Chrysomya rufifacies Macquart (Diptera: Calliphoridae) larvae in Case 2 and Case 3, respectively. Findings from this study are significant as they demonstrate that certain groups of fly can locate dead bodies even in high-rise buildings. Forensic entomofauna research on corpses found at high elevation is scarce and our study has highlighted the peculiarity of the fly species involved in Malaysia.

  16. Decomposition and dipteran succession in pig carrion in central Argentina: ecological aspects and their importance in forensic science.

    Science.gov (United States)

    Horenstein, M Battán; Linhares, A Xavier; De Ferradas, B Rosso; García, D

    2010-03-01

    Data on the insect species associated with corpse decomposition are particularly important for estimation of the post-mortem interval (PMI) in forensic science because the PMI is based on the lifecycle and behaviour of necrophagous insects, among other measures. To determine the dipteran succession on pig carrion, four experiments, one in each season, were carried out during 2004 in a rural area of Córdoba, central Argentina. Two pigs (Sus scrofa L.) were used in each of the four experiments. At each time-point one pig was placed in the shade and the other under direct sunlight. Insects were collected daily during the first 4 weeks and thereafter every 2 or 3 days. Five stages of decomposition were observed and a total of 24 710 adult specimens were collected, belonging to the following eight families of Diptera: Calliphoridae; Muscidae; Sarcophagidae; Phoridae; Piophilidae; Fanniidae; Sphaeroceridae, and Anthomyiidae. All Calliphoridae collected in this study were considered to be of potential forensic importance because of their necrophagous behaviour and because their immature stages use carrion as a food source. Other species, such as Musca domestica L. and Ophyra aenescens (Wiedemann), were also considered to represent potential forensic indicators.

  17. A Modified Trap for Adult Sampling of Medically Important Flies (Insecta: Diptera

    Directory of Open Access Journals (Sweden)

    Kamran Akbarzadeh

    2012-12-01

    Full Text Available Background: Bait-trapping appears to be a generally useful method of studying fly populations. The aim of this study was to construct a new adult flytrap by some modifications in former versions and to evaluate its applicability in a subtropical zone in southern Iran.Methods: The traps were constructed with modification by adding some equipment to a polyethylene container (18× 20× 33 cm with lid. The fresh sheep meat was used as bait. Totally 27 adult modified traps were made and tested for their efficacies to attract adult flies. The experiment was carried out in a range of different topographic areas of Fars Province during June 2010.Results: The traps were able to attract various groups of adult flies belonging to families of: Calliphoridae, Sarcophagidae, Muscidae, and Faniidae. The species of Calliphora vicina (Diptera: Calliphoridae, Sarcophaga argyrostoma (Diptera: Sarcophagidae and Musca domestica (Diptera: Muscidae include the majority of the flies collected by this sheep-meat baited trap.Conclusion: This adult flytrap can be recommended for routine field sampling to study diversity and population dynamics of flies where conducting of daily collection is difficult.

  18. Resiliência do retalho independente centenário de Lisboa

    OpenAIRE

    Rousseau, José António

    2013-01-01

    Como compreender os mecanismos ou os processos que, empresas retalhistas independentes de Lisboa, criadas ao longo do século XIX e algumas até anteriores, conseguiram dobrar o século XX e chegar aos dias de hoje ainda saudáveis, lucrativas e com potencial para o futuro? Tal situação só pode ser explicada pela fantástica capacidade de resiliência demonstrada na sua actividade ao longo de quase dois séculos. Mas o que é, no que consiste a resiliência e quais as suas capacidades e factores po...

  19. Hierarquia social e uso de sombra por vacas leiteiras

    OpenAIRE

    Pellizzoni, Camila

    2011-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Agroecossistemas, Florianópolis, 2011 As interações entre comportamento social e recursos ambientais, tais como a sombra, podem influenciar o bem-estar e o desempenho de vacas leiteiras criadas a pasto. Quando os recursos são limitados, a competição pode levar à exclusão de alguns animais, sendo que a qualidade do recurso em disputa também pode afetar a intensidade da ...

  20. Evolución en el tratamiento de las úlceras por presión

    OpenAIRE

    Pérez Niño, Sofía

    2015-01-01

    Las úlceras por presión suponen un gran problema en el ámbito sanitario por eso el objetivo de este trabajo es evaluar la evidencia científica disponible sobre la evolución en el tratamiento de las úlceras por presión. Es objeto de estudio la utilización de plantas, condimentos, resinas, aceites para el tratamiento de las úlceras y; la utilización de miel y larvas estériles criadas en laboratorios para limpiar los tejidos necrosados y no dañar el tejido sano; como los numerosos avances lograd...

  1. La Internacionalización de la reproducción la inserción laboral de la mujer inmigrante en los servicios de proximidad /

    OpenAIRE

    2002-01-01

    Consultable des del TDX Títol obtingut de la portada digiltalitzada Ciertamente, la mercantilización del trabajo doméstico-familiar siempre ha existido, de la mano de la figura de la criada o criado y del servicio doméstico tradicional. Lo que sí es nuevo es el contexto en el que surge el actual incremento masivo de los «servicios de proximidad», que pueden definirse como aquellas actividades remuneradas destinadas a satisfacer las necesidades de las personas y de las familias que surge...

  2. Parasitismo gastrintestinal em diferentes intensidades de pastejo no capim Tânzania, em caprinos

    OpenAIRE

    Silva, Helenara Machado da [UNESP

    2008-01-01

    Os objetivos gerais deste estudo visaram verificar o parasitismo gastrintestinal em diferentes intensidades de pastejo no capim Tanzânia (Panicum maximum (Jacq.) cv. Tanzânia-1), em caprinos e avaliar o método Famacha em cabras ¾ Boer ¼ Saanen e Saanen, criadas em sistema de pastejo, sob condições subtropicais. Foram utilizadas 65 cabras, sendo 21 cabras da raça Saanen e 44 cabras de composição genética ¾ Boer ¼ Saanen, pesando entre 35 a 40 kg. No tratamento de baixa intensidade de pastejo (...

  3. A Biblioteca da Real Academia dos Guardas-Marinha: a constituição e organização de um acervo especializado mediada pela formação profissional do oficialato militar-

    OpenAIRE

    2014-01-01

    Em 1802, era criada uma biblioteca para prover lentes e alunos da Real Academia dos Guardas-Marinha, instituição militar de ensino que formava o oficialato empregado na Marinha portuguesa, de saberes diversos relacionados com a atividade de uma marinha de guerra. Com a vinda daquela Academia junto com a Corte, a Biblioteca dos Guardas-Marinha tornou-se a primeira biblioteca pública a funcionar no Rio de Janeiro. Contudo, é a organização temática estabelecida pelo seu primeiro diretor que demo...

  4. Usos de si e (in)formalidade no trabalho da empregada doméstica diarista

    OpenAIRE

    Claudia Regina Barroso Ribeiro

    2014-01-01

    A tese investiga as dramáticas do uso de si e as normas do trabalho informal das empregadas domésticas diaristas, analisando como acontece a interação entre as normas definidas pelos empregadores e as novas normas, criadas pelas diaristas, para dar sentido às suas atividades, estabelecendo os modos de gestão do cotidiano. Trata-se de uma pesquisa predominantemente qualitativa e buscou analisar uma situação determinada, a das trabalhadoras diaristas da Região Metropolitana de Belo Horizonte. A...

  5. Resenha crítica do livro Escuela de la Guitarra: Exposición de la teoría instrumental de Abel Carlevaro

    Directory of Open Access Journals (Sweden)

    Aluísio Coelho Barros

    2014-07-01

    Full Text Available Esta resenha pretende apresentar e discutir alguns dos pontos abordados por Abel Carlevaro em seu livro Escuela de la Guitarra: Exposición de la teoría instrumental. O objetivo é de aproximar violonistas estudantes e profissionais à escola técnica de violão criada por Abel Carlevaro, já considerada uma das mais importantes escolas técnicas do século XX. Dentro dos limites de uma resenha, os principais assuntos abordados por Carlevaro são detalhadamente discutidos com uma abordagem crítica.

  6. Resenha crítica do livro Escuela de la Guitarra: Exposición de la teoría instrumental de Abel Carlevaro

    OpenAIRE

    Aluísio Coelho Barros

    2014-01-01

    Esta resenha pretende apresentar e discutir alguns dos pontos abordados por Abel Carlevaro em seu livro Escuela de la Guitarra: Exposición de la teoría instrumental. O objetivo é de aproximar violonistas estudantes e profissionais à escola técnica de violão criada por Abel Carlevaro, já considerada uma das mais importantes escolas técnicas do século XX. Dentro dos limites de uma resenha, os principais assuntos abordados por Carlevaro são detalhadamente discutidos com uma abordagem crítica....

  7. The symbolic surrealistic image in the poetry of Juan Eduardo Cirlot

    OpenAIRE

    2010-01-01

    Este artigo procura estudar as imagens surrealistas e a sua polivalência na poesia de Juan Eduardo Cirlot, um dos membros do grupo catalão Dau al Set e um dos mais interessantes críticos de arte em Espanha na segunda metade do século XX. Considerando que as imagens criam a sua própria realidade e exprimem uma necessidade espiritual de viver nessa realidade criada, o nosso propósito é o de compreender, através da profunda afinidade com o surrealismo onírico, a obsessão recorrent...

  8. EM BUSCA DA PALAVRA CONECTADA, IN-CORPORADA: A INSERÇÃO DA PSICOTERAPIA CORPORAL NO CAMPO PSICOLÓGICO DOS ANOS 80

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo Melo Oliveira

    2013-10-01

    Full Text Available Dentre as abordagens da psicologia clínica, a psicoterapia corporal teve uma rápida e significativa expansão no decorrer dos anos 80, nos grandes centros urbanos brasileiros. Essa difusão deu-se nas brechas criadas pelo boompsicanalítico que, desde os anos 70, criava espaços para a formação de uma cultura psicológica entre as camadas médias de nossa sociedade.

  9. EM BUSCA DA PALAVRA CONECTADA, IN-CORPORADA: A INSERÇÃO DA PSICOTERAPIA CORPORAL NO CAMPO PSICOLÓGICO DOS ANOS 80

    OpenAIRE

    2013-01-01

    Dentre as abordagens da psicologia clínica, a psicoterapia corporal teve uma rápida e significativa expansão no decorrer dos anos 80, nos grandes centros urbanos brasileiros. Essa difusão deu-se nas brechas criadas pelo boompsicanalítico que, desde os anos 70, criava espaços para a formação de uma cultura psicológica entre as camadas médias de nossa sociedade.

  10. Respuesta de Sigmodon hispidus (Rodentia: Muridae) a la inoculación experimental con el virus de la estomatitis vesicular serotipo New Jersey

    OpenAIRE

    Rodriguez, Julia; Dolz, Gaby; Jimenez, Ana Eugenia; Herrero, Marco Vinicio; Salman, Mo

    2013-01-01

    Se han reportado anticuerpos contra el Virus de Estomatitis Vesicular, serotipo New Jersey (VEV-NJ), en Sigmodon hispidus (rata algodonera) libres en Costa Rica. Con el fin de evaluar su respuesta ante el virus y su potencial rol como reservorio, treinta ratas algodoneras adultas, criadas en laboratorio, fueron inoculadas experimentalmente con 107 TCID50/ml VEV-NJ de la siguiente manera: 10 vía subcutánea (grupo A), 10 por abrasión oral (grupo B) y 10 por vía intranasal (grupo C). Se incluyer...

  11. Music finger reading

    OpenAIRE

    Fabiana Fator Gouvea Bonilha

    2006-01-01

    Resumo: O presente estudo foi motivado pela experiência pessoal de sua autora,musicista com deficiência visual. Ele aborda aspectos referentes ao ensino da Musicografia Braille, que por sua vez, consiste na notação musical criada por Louis Braille para uso de pessoas cegas. A partir de um enfoque qualitativo, buscou-se investigar a percepção de estudantes de Música com deficiência visual e de seus respectivos professores acerca das condições atuais de aplicação da Musicografia Braille ao c...

  12. Discriminação entre olhos normais e glaucomatosos mediante polarimetria de varredura a laser

    OpenAIRE

    Magacho,Leopoldo; Marcondes, Ana Maria; Costa,Vital Paulino

    2004-01-01

    OBJETIVOS: Avaliar a capacidade da polarimetria de varredura a laser de discriminar entre olhos normais e glaucomatosos. MÉTODOS: Cento e doze pacientes com glaucoma primário de ângulo aberto e 88 indivíduos normais foram incluídos no estudo. Todos os indivíduos foram submetidos a exame oftalmológico completo, perimetria computadorizada (Humphrey 24-2, full threshold), avaliando-se Mean Deviation (MD) e Correct Pattern Standard Deviation (CPSD) e exame com GDx. Curvas ROC foram criadas e novo...

  13. Preparo de vacina oleosa experimental contra bronqute infecciosa das galinhas e avaliação em aves gnotobióticas (GN) em aves livres de patógenos (SPF)

    OpenAIRE

    Dorileia Oliveira Resende

    1985-01-01

    Preparo de vacina oleosa experimental contra bronquite infecciosa das galinhas (big) e avaliação em aves gnotibióticas (gn) e em aves livres de patógenos (sff). Foi preparada uma vacina oleosa experimental, sob forma de emulsão dupla e testada em aves gn e em aves spf.oProduto final atingiu os requisitos de estabilidade, viscosidade e tipo de emulsão. As aves gn foram criadas em isoladores apropriados e divididas em dois grupos de quatro aves cada (vacinadas e não vacinadas). As aves spf fora...

  14. Transformações socioculturais da alimentação hospitalar

    OpenAIRE

    Godoy,Andresa Michele; Lopes,Doraci Alves; Garcia, Rosa Wanda Diez

    2007-01-01

    O artigo busca no passado os elementos constituintes da cultura alimentar hospitalar, com o objetivo de encontrar as raízes históricas da alimentação em instituições hospitalares. Pressupõe-se que a dieta não seja apenas resultante de procedimentos tecnocientíficos, mas também historicamente construída, criada e recriada perante aspectos econômicos e socioculturais. Realizou-se um mapeamento inicial em arquivos universitários, municipais e hospitalares de Campinas (SP), além de entrevistas co...

  15. Plano de Marketing : Iogurteria do Bairro 2014

    OpenAIRE

    Caupers, Margarida Maria Vargas Firmino e Goes

    2013-01-01

    Mestrado em Marketing O presente trabalho de projecto traduz-se na realização de um Plano de Marketing para a Iogurteria do Bairro. Esta empresa foi criada por dois amigos e sócios e foi lançada em Março de 2012 no mercado da restauração, mais propriamente em frozen yogurt. Este Plano de Marketing tem como objectivos aumentar o reconhecimento da marca perante os clientes, fidelizar os clientes existentes e angariar clientes novos e aumentar as vendas e a notoriedade da marca perante os con...

  16. STRUCUTRAL ORGANIZATION OF A REGULAMENTORY AGENCY: CASE STUDY

    OpenAIRE

    DIANA KELMAN FRAJTAG

    2002-01-01

    O modelo de Mintzberg das cinco configurações estruturais é útil para a classificação das organizações e para a identificação de suas tendências evolutivas. O modelo serviu de suporte para uma análise crítica da estrutura adotada pela Agência Nacional de Águas - ANA. A ANA foi criada pela lei 9984/2000 com a missão de, simultaneamente, regular um bem de uso público - o uso dos rios - e implementar o Sistema Nacional de Gerenciamento de Recursos Hídricos. A ANA te...

  17. Florística, fitossociologia e aspectos da dinâmica de um remanescente de mata da encosta no Morro Santana, Porto Alegre, Rio Grande do Sul

    OpenAIRE

    Deize de Vargas

    2005-01-01

    A cidade de Porto Alegre, devido à localização, abriga espécies vegetais procedente de dois corredores principais: o corredor Atlântico e o corredor do Alto Uruguai. Os morros graníticos sobressaem-se na paisagem porto-alegrense abrigando matas nas suas encostas sul e campos nos topos e encostas nortes, devido às condições criadas pela exposição solar diferenciada. O Morro Santana, com 311 metros é o mais alto deles. Com o objetivo de caracterizar a composição e a estrutura e aspectos da dinâ...

  18. Uso do teste de Scott-Knott e da análise de agrupamentos, na obtenção de grupos de locais para experimentos com cana-de-açúcar

    OpenAIRE

    Cristiane Mariana Rodrigues da Silva

    2008-01-01

    O Centro de Tecnologia Canavieira (CTC), situado na cidade de Piracicaba, é uma associação civil de direito privado, criada em agosto de 2004, com o objetivo de realizar pesquisa e desenvolvimento em novas tecnologias para aplicação nas atividades agrícolas, logísticas e industriais dos setores canavieiro e sucroalcooleiro e desenvolver novas variedades de cana-de-açúcar. Há 30 anos, são feitos experimentos, principalmente no estado de São Paulo onde se concentra a maior parte dessas unidades...

  19. A diplomacia das cátedras: a política cultural externa alemã e o ensino superior paulista - os casos da USP e da Escola Paulista de Medicina (1934-1942

    Directory of Open Access Journals (Sweden)

    André Felipe Cândido da Silva

    2013-06-01

    Full Text Available O artigo trata da participação da diplomacia alemã nas negociações relativas à contratação de professores para a USP, criada em 1934, e para a Escola Paulista de Medicina, do ano anterior. Ao mesmo tempo em que a diplomacia do Terceiro Reich procurou garantir as cátedras para os alemães, a origem judaica dos nomeados para a USP envolveu conflitos e impasses, que não ocorreram com a mesma intensidade no caso do patologista Walter Büngeler, contratado pela escola médica em 1936.

  20. Um dilema feminino: o câncer de colo do útero

    OpenAIRE

    AMORIM, Linamar Teixeira de

    2009-01-01

    O tratamento do câncer do colo do útero, uma doença com desenvolvimento lento, é eficaz, principalmente nas lesões precursoras. Para compreender essa doença, na perspectiva da sociologia aplicada à saúde, realizou-se um estudo com mulheres que vivenciaram a experiência do câncer uterino, mediante entrevista semiestruturada, visando apreender as representações criadas por elas. As entrevistas revelaram a coexistência tanto de aspectos positivos quanto negativos na experiência com a doenç...

  1. Divulga????o do Parque Ecol??gico Bernardo Say??o

    OpenAIRE

    Lima, La??s de Souza

    2011-01-01

    O atual estilo de vida da sociedade vem afastando cada vez mais o homem da natureza, o que justifica as crises ambientais hoje existentes. As Unidades de Conserva????o bem como as atividades de educa????o ambiental nelas desenvolvidas, foram criadas a fim de conservar a biodiversidade. O objetivo geral do presente trabalho foi sensibilizar uma comunidade urbana para a no????o de que o homem faz parte da natureza e ambos estabelecem uma rela????o de m??tua intera????o, os objetivos espec??fico...

  2. Sonhos de Nabucodonosor: aspectos da propaganda do Estado Novo Pernambucano

    OpenAIRE

    2005-01-01

    Este trabalho tem por objetivo explorar as diversas formas e imagens criadas e utilizadas pela propaganda oficial do Estado Novo pernambucano durante seu período de vigência, entre 1937 e 1945, mais particularmente em seus primeiros cinco anos, a grosso modo, o período em que o Brasil ainda não se envolvera na II Guerra Mundial. Durante estes oito anos, Pernambuco foi governado pelo interventor federal Agamenon Magalhães. A partir da análise do que ainda resta do vasto mater...

  3. Utilizando resultados de atividades com tecnologias digitais como elementos constituintes da avaliação: uma experiência na disciplina de biologia no ensino médio

    OpenAIRE

    Faria, Kely Cemin

    2011-01-01

    Este volume apresenta o resultado da pesquisa associada ao trabalho da Dissertação de Mestrado da autora, intitulado “Utilizando resultados de atividades com tecnologias digitais como elementos constituintes da avaliação: uma experiência na disciplina de biologia no ensino médio”, o qual teve por objetivo investigar as contribuições e possibilidades da construção de Blogs, elaboração/utilização de vídeos digitais e atividades numa sala de aula virtual criada no ambiente Moodle como elementos ...

  4. Fatores da embalagem de café orgânico torrado e moído na intenção de compra do consumidor

    OpenAIRE

    Suzana Maria Della Lucia; Valéria Paula Rodrigues Minim; Carlos Henrique Osório Silva; Luis Antonio Minim

    2007-01-01

    O efeito de algumas características da embalagem de café orgânico sobre a intenção de compra do consumidor foi avaliado, utilizando-se a conjoint analysis. Quatro características, cada uma com dois níveis, foram manipuladas: preço do produto (alto e baixo), cor da embalagem (vermelha e verde), marca (conhecida e desconhecida) e informação sobre orgânicos (com e sem os dizeres "produto isento de agrotóxicos" e "não agride o meio ambiente"). Dezesseis embalagens hipotéticas foram criadas seguin...

  5. Avaliação da intenção de compra de couve minimamente processada Consumer intention to purchase for minimally processed cabbage

    OpenAIRE

    Dantas,Maria I. S.; Rosires Deliza; Minim,Valéria P.R.; Duncan Hedderley

    2005-01-01

    Vários fatores influenciam a percepção do produto pelo consumidor e, conseqüentemente, sua intenção de compra. Dentre tais fatores pode estar a expectativa criada pelas características da embalagem e do rótulo, pois representam o primeiro contato entre o indivíduo e o produto. O objetivo desse trabalho foi avaliar o efeito das características da embalagem na intenção de compra de couve minimamente processada, considerando as diferenças individuais dos consumidores para processar as informaçõe...

  6. Redes sociais virtuais de informação sobre amor: comportamento e cultura informacional de usuários do Orkut

    OpenAIRE

    Ruleandson do Carmo Cruz

    2011-01-01

    Estuda as redes sociais virtuais de informação sobre amor formadas por usuários do site Orkut, focando-se no comportamento e na cultura informacional de tais usuários, bem como na representação social do amor por eles criada. Tem como objeto de estudo cinco das comunidades virtuais sobre amor existentes no referido site: "Se é amor q... seja verdadeiro!", "Amor e sexo em debate", "Eu ACREDITO no amor", "Amor, Respeito e Confiança" e "O mito do amor romântico". Considera como universo empírico...

  7. Preparo de vacina oleosa experimental contra bronqute infecciosa das galinhas e avaliação em aves gnotobióticas (GN) em aves livres de patógenos (SPF)

    OpenAIRE

    Dorileia Oliveira Resende

    1985-01-01

    Preparo de vacina oleosa experimental contra bronquite infecciosa das galinhas (big) e avaliação em aves gnotibióticas (gn) e em aves livres de patógenos (sff). Foi preparada uma vacina oleosa experimental, sob forma de emulsão dupla e testada em aves gn e em aves spf.oProduto final atingiu os requisitos de estabilidade, viscosidade e tipo de emulsão. As aves gn foram criadas em isoladores apropriados e divididas em dois grupos de quatro aves cada (vacinadas e não vacinadas). As aves spf fora...

  8. CLIMA ORGANIZACIONAL: MODELO DE PESQUISA DOS FATORES DE SATISFAÇÃO NO TRABALHO E AMBIENTE ORGANIZACIONAL

    OpenAIRE

    Laís Novaes Pillar de Oliveira Castro; Túlio Baita dos Reis; Everton Luis Pinheiro Dos Santos; George Silva Machado; Rosana Candiano Rangel

    2016-01-01

    As organizações são sistemas sociais criadas com a função de suprir alguma necessidade do homem e, para isso, precisam de uma estrutura com pessoas nelas envolvidas. Este projeto teve por objetivo, desenvolver um instrumento de pesquisa de clima organizacional a fim de demonstrar a importância do clima organizacional no que se refere à satisfação dos colaboradores nas organizações. O estudo buscou, por meio da literatura existente, explicar as complexidades das organizações e das pessoas como...

  9. A relação da reputação com a satisfação de clientes, jornalistas e colaboradores : o caso do Grupo Altis Hotels

    OpenAIRE

    2011-01-01

    Mestrado em Marketing A atitude dos públicos internos e externos para com uma empresa é influenciada pela forma como percepcionam a sua reputação. Especialmente no sector de serviços, onde a experiência dos públicos externos é criada pela interacção com os colaboradores, as perspectivas dos diferentes públicos são vistas como interdependentes. Por outro lado, a percepção de líderes de opinião como os jornalistas, cuja função é reportar sobre as empresas, influencia as atitudes dos outros p...

  10. A experiência da Rede de Agroecologia do Leste Paulista (SP) – Brasil

    OpenAIRE

    Urchei,Mário Artemio; Corrales, Francisco Miguel; Canuto, João Carlos; Queiroga, Joel Leandro de; Braga, Kátia Sampaio Malagoli; Camargo,Ricardo Costa Rodrigues de; Ramos, Myrian Suely Teixeira Alves dos Santos; Ramos Filho, Luiz Octavio

    2015-01-01

    A Rede de Agroecologia do Leste Paulista (SP) – Brasil foi criada em 2005 a partir de uma articulação entre Agricultores(as) Familiares da região e de um conjunto de organizações governamentais e não governamentais de ensino, pesquisa e extensão com o objetivo de contribuir para o desenvolvimento local sustentável fundamentado nos princípios da Agroecologia. Para isso, as estratégias desenvolvidas pela Rede ao longo desses dez anos foram pautadas por processos participativos voltados à constr...

  11. Narrar, mostrar, ver: como analisar um álbum de fotos de família em conexão com uma entrevista narrativa = Narrating, showing, seeing: how to analyze a family photo album in connection with a narrative interview

    OpenAIRE

    2014-01-01

    Álbuns de fotos de família são lugares onde memórias são criadas e fixadas (Chalfen, 1987). Eles consistem principalmente de fotografias e outros tipos de material visual (cartões postais, bilhetes ou ingressos etc. ), bem como de legendas e comentários mais ou menos extensos. Tradicionalmente, os retratos são organizados em um livreto, isto é, em uma ordem sequencial que se baseia implicitamente em uma estrutura pictórica e narrativa de apresentação e criação de uma “moldura familiar” (Hirsc...

  12. Investigando Jean Piaget : a epistemologia genética e o apriorismo

    OpenAIRE

    Rizzon, Gisele

    2009-01-01

    O presente estudo constitui-se na Dissertação de Mestrado apresentada ao Programa de Pós-Graduação da Universidade de Caxias do Sul, pertencente à linha de pesquisa Educação, Epistemologia e Linguagem, e ancora-se nos pressupostos teóricos e conceituais da Epistemologia Genética. Essa teoria, criada pelo pensador suíço Jean Piaget, com o fim investigativo de entender os processos cognitivos, mais especificamente, no que se direciona a revelar a gênese do conhecimento. O intuito principal do e...

  13. o papel das TIC no exercício da cidadania dos adultos

    OpenAIRE

    Álvaro, Ana Margarida Domingues

    2011-01-01

    Numa sociedade profundamente marcada pela tecnoogia e pela globalização, novos desafios se colocam aos indivíduos que deverão estar preparados para uma nova cidadania - a cidadania digital. Começámos por enquadrar a sociedade da informação e a transição para a sociedade do conhecimento, no sentido em que não basta ter acesso à informação é preciso transformá-la em conhecimento efetivo. Contudo apesar de todas as oportunidades criadas pela tecnologia, há ainda o problema da exclusão social e d...

  14. A escrava Isaura : uma visão multidimensional

    OpenAIRE

    Guimarães Caminha Neto, José

    2003-01-01

    Este trabalho busca revelar os muitos segredos que se escondem por trás das representações de uma escrava mestiça, nascida sob o signo do Romantismo, chamada Isaura. A personagem criada por Bernardo Guimarães na segunda metade do século XIX revela-se cheia de contradições que, nesta pesquisa, se explicam nas teorias pós-modernas. Assim como o Folhetim, gênero híbrido criado da união do Jornalismo Impresso com a Literatura, Isaura sofre o preconceito de não ser aceita pela so...

  15. O imperialismo, fase contemporanea do capitalismo

    OpenAIRE

    Benitez, Jose Leandro Farias

    1990-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina. Centro de Ciencias Juridicas Esta dissertação analisa o imperialismo segundo a teoria criada por Lênin e tenta mostrar sua validade na interpretação das manifestações contemporâneas do fenômeno. O método utilizado é o indutivo. A obra de Lênin "O Imperialismo, Fase Superior do Capitalismo" deu origem à teoria marxista-leninista do imperialismo, que é caracterizado, como resultante da concentração da produção e do capital que...

  16. Conceitos alternativos sobre radicais livres: origens, prevalência e intervenções

    OpenAIRE

    Rosiris Sindeaux de Alencar Pires de Oliveira

    2016-01-01

    Este trabalho teve por objetivos: levantar conceitos alternativos sobre radicais livres na mídia impressa e em livros de Bioquímica; examinar os conceitos alternativos prevalentes em pós-graduandos e em professores de Química e Biologia do Ensino Médio; e, planejar e aplicar intervenção para reduzir a influência destes conceitos. Para o levantamento na mídia impressa, foi selecionada a revista Veja, período de 01/01/2000 a 31/07/2014, e foram criadas as seguintes categorias para classificar o...

  17. Elementos do Projeto Político Profissional da Associação Nacional das Enfermeiras Diplomadas Brasileiras presentes nos Annaes de Enfermagem Elementos del Proyecto Politico Profesional de la Asociación Nacional de Enfermeras Graduadas Brasileras presentes en los Annaes de Enfermagem Elements of the Political Professional Project of the Brazilian Graduate Nurses National Association present in the Annaes de Enfermagem

    OpenAIRE

    Jane Liliane Gonçalves da Cruz; Isaac Rosa Marques

    2006-01-01

    A revista Annaes de Enfermagem foi criada para publicar idéias, conceitos, resultados da produção científica, reflexões e, principalmente, o de expor e discutir o Projeto Político Profissional da Associação Nacional de Enfermeiras Diplomadas Brasileiras (ANEDB). Este estudo teve como objetivo descrever e caracterizar estes elementos presentes na referida revista no período de 1932 a 1941. Trata-se de um estudo de abordagem qualitativa pelo método histórico. Os principais elementos do projeto ...

  18. A PÓS-GRADUAÇÃO COMO CENÁRIO SOCIAL DE CONSTITUIÇÃO DE SUBJETIVIDADES

    OpenAIRE

    Ataídes, Cláudia Barrozo de Queiroz

    2005-01-01

    Esta pesquisa investiga a pós-graduação apresentando-a como cenário social de constituição de subjetividades. A partir de indicadores produzidos em encontros mensais com nove mestrandos, realizados entre os meses de março de 2004 e maio de 2005, a subjetividade social, categoria criada por González Rey (2003) e as forças sociopsicológicas, em especial a sociometria, categoria proposta por Moreno (1992), são exploradas com o intuito de ampliar a reflexão sobre os processos so...

  19. A Comunicação no Cinema dos Sentidos: Abordando a Imersão sob a Perspectiva do Som

    OpenAIRE

    OPOLSKI, Débora Regina

    2015-01-01

    O presente trabalho pretende abordar a imersão criada pelo Cinema dos Sentidos sob a perspectiva do som. De acordo com Robert Stam (2011), no Cinema dos Sentidos, a sensação predomina sobre a narrativa e o som cinematográfico aparece como um elemento capaz de aumentar a sensação de realidade da imagem. A criação da imersão é discutida com base em três aspectos sonoros: o estabelecimento do ponto de escuta; o som no supercampo em conjunto com as questões da espacialidade sonora e; o hiper-real...

  20. A tecelagem das margens: Por que tão solo? Dança e dramaturgia

    OpenAIRE

    Tarcisio dos Santos Ramos

    2008-01-01

    Esta dissertação tem como proposta central a investigação da dramaturgia do espetáculo POR QUE TÃO SOLO?, criado em Belo Horizonte, no ano de 2006, pela Marcenaria Centro de Criação Cênica, como resultado do Prêmio Klauss Vianna de Dança/Funarte. O principal objetivo da pesquisa é identificar os elementos dramatúrgicos constitutivos do espetáculo e discutir seu caráter dialógico e as conexões criadas durante o processo criativo. Pretende-se, ainda, revelar os mecanismos que contribuíram para...

  1. Gestão Multiprofissional em Gerontologia: do Tratamento Farmacológico às Intervenções Psicossociais

    OpenAIRE

    Paulo Renato Canineu; Paola Renata Brandão Canineu Bizar; Rafael Fernando Brandão Canineu

    2010-01-01

    Com o crescimento da população idosa brasileira, é cada vez mais importante formar profissionais que saibam conduzir o processo de envelhecimento. A Gerontologia é uma ciência relativamente nova, estando ainda em desenvolvimento desde que foi criada. Há mudanças no perfil dos gerontólogos e geriatras brasileiros nos últimos anos, favorecendo uma ação mais eficiente em relação a esta realidade, principalmente com a visão da interdisciplinaridade, propiciando assim, ir muito além da utilização ...

  2. Título da página eletrónica: SVAC ‑ International Research Group “Sexual Violence in Armed Conflict”

    Directory of Open Access Journals (Sweden)

    Tatiana Moura

    2013-02-01

    Full Text Available A rede internacional Sexual Violence in Armed Conflict (SVAC, criada oficialmente em 2010, reúne um conjunto de investigadoras de diversas áreas científicas (historiadores/as, sociólogos/as, juristas, filósofos/as, psicólogos/as, especialistas das área da literatura e dos estudos culturais, etc. e de ativistas dos direitos humanos que trabalham sobre questões relacionadas com a violência sexual em conflitos armados. O projeto inicial partiu das investigadoras do Hamburger Institut für Sozia...

  3. Fatores da embalagem de café orgânico torrado e moído na intenção de compra do consumidor Organic coffee packaging factors on consumer purchase intention

    OpenAIRE

    Suzana Maria Della Lucia; Valéria Paula Rodrigues Minim; Carlos Henrique Osório Silva; Luis Antonio Minim

    2007-01-01

    O efeito de algumas características da embalagem de café orgânico sobre a intenção de compra do consumidor foi avaliado, utilizando-se a conjoint analysis. Quatro características, cada uma com dois níveis, foram manipuladas: preço do produto (alto e baixo), cor da embalagem (vermelha e verde), marca (conhecida e desconhecida) e informação sobre orgânicos (com e sem os dizeres "produto isento de agrotóxicos" e "não agride o meio ambiente"). Dezesseis embalagens hipotéticas foram criadas seguin...

  4. Efeitos da temperatura e da umidade relativa sobre o desempenho e o rendimento de cortes nobres de frangos de corte de 1 a 49 dias de idade

    OpenAIRE

    Oliveira,Rita Flávia Miranda de; Donzele,Juarez Lopes; Abreu,Márvio Lobão Teixeira de; Ferreira,Rony Antônio; Vaz,Roberta Gomes Marçal Vieira; Cella,Paulo Segatto

    2006-01-01

    Foram utilizados 180 pintos machos de 1 dia de idade, da linhagem Ross (peso inicial 43,0 ± 0,2 g) para avaliação dos efeitos de diferentes ambientes sobre o desempenho e as características de carcaça. As aves foram criadas no período de 1 a 49 dias de idade em um delineamento experimental inteiramente casualizado, com três ambientes (conforto térmico, calor seco e calor úmido), seis repetições por tratamento e dez aves por unidade experimental. As rações e a água foram fornecidas à vo...

  5. Acompanhamento terapêutico e clínica das psicoses

    Directory of Open Access Journals (Sweden)

    Lúcia Grossi dos Santos

    Full Text Available O artigo apresenta o acompanhamento terapêutico como modalidade clínica no tratamento da psicose, criada a partir da reforma psiquiátrica e que vem se firmando no contexto da desospitalização psiquiátrica. Além de um estudo histórico do surgimento desse dispositivo clínico, o trabalho pretende discutir as funções do acompanhamento terapêutico, a sua particularidade dentro do trabalho de equipe no tratamento da psicose e seu papel na reinserção social do psicótico.

  6. EMBRAFILME : a estrutura de comercialização na gestão Roberto Farias (1974-1979)

    OpenAIRE

    Sergio Ribeiro de Aguiar Santos

    2003-01-01

    Resumo: Esse projeto visa resgatar um período do cinema brasileiro através da EMBRAFILME (Empresa Brasileira de Filmes S.A) no que diz respeito às atividades de comercialização da empresa, criada a partir da gestão do cineasta Roberto Farias (1974-1979) como diretor - geral da Empresa. o trabalho visa resgatar a participação da Empresa no cenário cinematográfico brasileiro como executora da política nacional de distribuição de filmes no Brasil e sua comercialização no exterior, buscando avali...

  7. O educador social P.e Joaquim Alves Brás e a obra de Santa Zita

    OpenAIRE

    Martins, Ernesto

    2010-01-01

    A presente investigação historico-descritiva e documental sobre a Obra de Santa Zita, do seu fundador (Joaquim Alves Brás) expresso nas suas ações e pensamento, pretende valorizar a “criada de servir” ou empregada doméstica desde o Estado Novo. Esta preocupação social e humana pelas empregadas domésticas, pretende dignificar pessoal e socioprofissional, do papel destas pessoas no trabalho no seio da familia portuguesa. Esta obra, com as suas instituições e valências, foram promovendo a mulher...

  8. A Vitivinicultura na região do Alentejo: A passagem de um setor tradicional para um setor inovador: O caso da sub-região vitivinícola de Reguengos de Monsaraz

    OpenAIRE

    Correia, Ana Sofia Paixão

    2016-01-01

    O setor vitivinícola sofreu uma grande transformação na região do Alentejo. Foram criadas importantes iniciativas de dinamização do setor vitivinícola, através do apoio prestado por associações, empresas e instituições regionais. A sub-região vitivinícola de Reguengos de Monsaraz é um forte exemplo do crescimento que ocorreu na vitivinicultura. Os produtores apostaram na inovação para melhorarem a cultura da sua vinha e a produção dos seus vinhos. Reguengos de Monsaraz apresenta um e...

  9. Fatores de risco, etiologia e aspectos clínicos da mastite em ovelhas de corte no Paraná

    OpenAIRE

    Priscilla F. V. Pereira; Stotzer,Erika S.; Lucienne G. Pretto-Giordano; Ernst E. Müller; Júlio A. N. Lisbôa

    2014-01-01

    A mastite em ovelhas da raça Santa Inês apresenta-se como um problema de grande proporção e gravidade e é dificilmente tratada com sucesso. O objetivo deste estudo foi caracterizar aspectos clínicos, epidemiológicos e etiológicos da mastite clínica em ovelhas de corte criadas no norte do Paraná. O presente estudo foi realizado entre os meses de outubro de 2009 a setembro de 2010 envolvendo 54 rebanhos de ovinos de corte de diferentes raças. Durante as visitas às propriedades, um questionário ...

  10. Redes sociais virtuais de informação sobre amor: comportamento e cultura informacional de usuários do Orkut

    OpenAIRE

    Ruleandson do Carmo Cruz

    2011-01-01

    Estuda as redes sociais virtuais de informação sobre amor formadas por usuários do site Orkut, focando-se no comportamento e na cultura informacional de tais usuários, bem como na representação social do amor por eles criada. Tem como objeto de estudo cinco das comunidades virtuais sobre amor existentes no referido site: "Se é amor q... seja verdadeiro!", "Amor e sexo em debate", "Eu ACREDITO no amor", "Amor, Respeito e Confiança" e "O mito do amor romântico". Considera como universo empírico...

  11. Historias de dos desgraciadas. Estereotipos de la culpa en la literatura popular española de los siglos XVIII y XIX

    OpenAIRE

    Sinclair, Alison

    2016-01-01

    En este artículo se contrastan los casos de dos mujeres a quienes se les echaba la culpa de hacer mal, y que como consecuencia se veían calificadas de «desgraciadas»: Rosaura de Trujillo, víctima de violación, y Teresa, la criada castigada por Dios por su falta de caridad para con los pobres. Ambos casos pertenecen al género de los pliegos sueltos, forma de literatura popular en España, su origen es el siglo XVIII, y volvieron a publicarse repetidas veces hasta bien entrado el XIX. Al caer fu...

  12. Redimensionando a noção de aprendizagem nas relações entre perfil conceitual e contexto: uma abordagem sócio-cultural-histórica

    OpenAIRE

    Andre Machado Rodrigues

    2009-01-01

    Nesta dissertação apresentamos um estudo teórico que visa, a partir de uma perspectiva sócio-cultural-histórica, rever o modelo cognitivo de perfil conceitual inserindo em sua estrutura a noção de contexto. Para realizar esta conciliação teórica entre perfil conceitual e contexto, nos utilizamos da teoria da atividade e dos ferramentais teóricos que ela dispõe para entender o uso e a formação de conceitos. A teoria criada por Vigotski e posteriormente desenvolvida por Leontiev e Engeström som...

  13. Margaret Atwood: la sirena de géneros

    OpenAIRE

    Freire, Espido

    2009-01-01

    En la narrativa de Margaret Atwood la sorpresa, el uso poético del lenguaje y la reconstrucción constante de la identidad de los personajes guían al lector a traves de los distintos textos. Tanto en la reconstrucción de Los diarios de Susanna Moodie como en la traducción de emociones y sentimientos de la reina de Dinamarca en «La respuesta de Getrudis», la figura de las protagonistas femeninas fuertes e imprevisibles reinventan el misterio del eterno femenino. Las criadas y...

  14. O Casamento e a TPM: novos tempos, novos sentidos

    Directory of Open Access Journals (Sweden)

    Gabriela Boemler Hollenbach

    2003-12-01

    Full Text Available O objetivo do trabalho é analisar a forma como a revista TPM, Trip Para Mulher, criada para ser uma alternativa às revistas femininas tradicionais, trata do tema casamento. A partir dos pressupostos teóricos da Análise de Discurso Francesa, a investigação procurou encontrar os sentidos dominantes a respeito do casamento nas matérias publicadas na revista entre Abril e Julho de 2003, concluindo que o casamento ainda é considerado importante e desejável para as mulheres.

  15. Pedro Abelardo e São Bernardo de Claraval: duas formas conflitantes (?) de conhecer o mesmo Deus

    OpenAIRE

    Dias, Cléber

    2009-01-01

    O presente texto analisa as formas como Pedro Abelardo e S. Bernardo apresentam Deus para verificar se elas são conflitantes ou não. Enquanto que Pedro Abelardo propõe a possibilidade do conhecimento de Deus por via racional a partir de argumentos cosmológicos e da investigação sobre os atributos divinos, Bernardo de Claraval refuta o uso da lógica/dialética para o conhecimento de Deus. Se bem que S. Bernardo também vê na consideração sobre as coisas criadas e nos atributos divinos um modo de...

  16. Substratos utilizados para a criação de dípteros caliptratos no jardim zoológico do Rio de Janeiro (Rio-Zoo) Breeding media used for rearing flies in the Zoological Garden of Rio de Janeiro (Rio - Zoo)

    OpenAIRE

    José Mario d'Almeida

    1989-01-01

    No Rio-Zoo, foram criados 7.377 dípteros caliptratos, pertencentes a quatro famílias e 22 espécies. As criações foram efetuadas de agosto de 1987 a abril de 1988. Foram utilizados como substratos de criação: banana, camarão, carcaça de camundongo,fezes caninas, fezes humanas, fígado bovino, lula, mamão, peixe, siri e tomate. As espécies criadas com maior freqüência foram: Fannia sp. (subgrupo pusio), Atherigona orientalis, Chrysomyia megacephala, Phaenicia eximia. Paraphrissopoda chrysosotoma...

  17. La Salle e seu projeto educativo

    OpenAIRE

    Marcos Corbellini

    2011-01-01

    Resumo No dia 6 de junho de 1694 foi criada a Sociedade das Escolas cristãs, integrada por treze cidadãos, entre eles um sacerdote, João Batista de La Salle. Para buscar sua finalidade de manter escolas gratuitas para os filhos dos artesãos e dos pobres, a sociedade produziu muitos discursos, através dos quais explicitou suas concepções de vida e sua maneira de conduzir as escolas. Entre esses escritos estão as Cartas. Tem-se conhecimento de 133 cartas redigidas por La Salle, das quais 110 di...

  18. Modelo brasileiro de treinamento expert de saltos ornamentais

    OpenAIRE

    Márcia Cristina Custódia Ferreira

    2012-01-01

    O desenvolvimento do indivíduo até alcançar a expert performance, fundamenta-se na prática deliberada com professores especializados. Os futuros experts precisam adquirir mecanismos de monitoramento e controle para avaliar sua performance para poder gradualmente modificar seus métodos e ampliar as suas habilidades. Baseado no método de investigação criada por Ericsson e Simon (1984), Ericsson e Oliver (1988), este estudo procurou descobrir, à partir da aplicação do Protocolo de Análise em tre...

  19. Effects of rphedrine on cardiovascular and renal function of dogs anesthetized with sodium pentobarbital

    OpenAIRE

    Rosa Beatriz Amorim; José Reinaldo Cerqueira Braz; Yara Marcondes Machado Castiglia; Pedro Thadeu Galvão Vianna; Norma Sueli Pinheiro Módolo

    2002-01-01

    JUSTIFICATIVA E OBJETIVOS: Com a perspectiva criada por algumas pesquisas de ações diferenciadas da efedrina sobre a função renal, dependendo da dose utilizada, e considerando-se as controvérsias ainda existentes a respeito dos seus efeitos sobre a função renal, esta pesquisa experimental tem como objetivo verificar se doses diferentes de efedrina determinam efeitos hemodinâmicos e renais diferenciados. MÉTODO: Em 32 cães anestesiados com pentobarbital sódico (PS), submetidos a preparação cir...

  20. Efeitos da efedrina sobre as funções cardiovascular e renal de cães sob anestesia com pentobarbital sódico

    OpenAIRE

    Amorim, Rosa Beatriz [UNESP; Braz, José Reinaldo Cerqueira; Castiglia, Yara Marcondes Machado [UNESP; Vianna, Pedro Thadeu Galvão [UNESP; Módolo, Norma Sueli Pinheiro

    2002-01-01

    JUSTIFICATIVA E OBJETIVOS: Com a perspectiva criada por algumas pesquisas de ações diferenciadas da efedrina sobre a função renal, dependendo da dose utilizada, e considerando-se as controvérsias ainda existentes a respeito dos seus efeitos sobre a função renal, esta pesquisa experimental tem como objetivo verificar se doses diferentes de efedrina determinam efeitos hemodinâmicos e renais diferenciados. MÉTODO: Em 32 cães anestesiados com pentobarbital sódico (PS), submetidos a preparação cir...

  1. Física do meio ambiente: desafios de uma disciplina focada na formação de habilidades gerais

    OpenAIRE

    2013-01-01

    A disciplina Física do Meio Ambiente (FMA) foi criada em 1976 no Departamento de Física Teórica e Experimental da UFRN e, atualmente, faz parte da estrutura curricular do curso de Licenciatura em Física da UFRN e é de caráter obrigatório. Tal caráter se justifica, dentre outros motivos, pelo fato de que esta disciplina representa uma boa oportunidade dos licenciandos estudarem de que forma a Física pode contribuir para a compreensão de fenômenos relativos ao nosso Meio Ambiente, sejam eles re...

  2. Contribuição para o estudo do custo unitário das análises laboratoriais e sua comparação com a tabela de procedimentos da Associação Médica Brasileira - AMB 92, em um laboratório de pequeno porte

    OpenAIRE

    Gisele Palo Corrêa de Freitas

    2005-01-01

    O Laboratório de Análises Clínicas (LAC) vem buscando, no decorrer dos anos, alternativas quanto à sua capacidade em gerar receita. A busca por melhores resultados incrementou a parceria com as organizações chamadas de \\"convênios médicos\\" ou \\"medicina de grupo\\" que, em geral, remuneram as análises laboratoriais com base em tabelas de procedimentos criadas pela Associação Médica Brasileira AMB. Destas, a mais utilizada é a Tabela AMB 92, devido a utilizar valores de Coeficiente de Honorári...

  3. Thomas Ewbank: visão de hospitalidade de um turista republicano no Brasil Império

    Directory of Open Access Journals (Sweden)

    João dos Santos Filho

    2011-11-01

    Full Text Available Escritor, inventor, etnólogo, cientista e desenhista Thomas Ewbank inglês radicado nos Estados Unidos, desembarca no Rio de Janeiro em primeiro de fevereiro de 1846. Com seus 54 anos de idade, para visitar seu irmão mais velho Joseph Ewbank protestante, natural de Castel Barnard, Durham, Inglaterra, casado com a brasileira Eufrasia Marques Lisboa e residindo na Rua do Catete próximo ao Largo do Machado. Fundador em 17 de fevereiro de 1833 da primeira Loja maçônica brasileira que trabalha no Rito inglês a Orphan Lodge, criada no Rio de Janeiro.

  4. A utilizaçao da droga Halquinol® como promotor de crescimento e coadjuvante no controle da coccidiose

    OpenAIRE

    Cardoso, Maria Antoanete Brandalize; Flemming, Jose Sidney, 1949-

    2013-01-01

    O principal objetivo deste trabalho foi o estudo da influência da droga halquinol (Roxolin 60) como promotor de crescimento, bem como sua possível ação no controle da coccidiose, em frangos de corte. Foram analisados parâmetros como ganho de peso, conversão alimentar, viabilidade e índice de eficiência produtiva. Foram constituídos seis tratamentos, num total de 2.880 aves criadas em piso, com diferentes níveis da droga na ração. Observou-se que a utilização de halquinol como promotor de cres...

  5. Desenvolvimento do predador Podisus nigrispinus alimentado com Spodoptera frugiperda e Tenebrio molitor.

    OpenAIRE

    Oliveira,Harley Nonato de; Pratissoli, Dirceu; Pedruzzi,Eder Pin; Espindula,Marcelo Curitiba

    2004-01-01

    Ninfas de Podisus nigrispinus (Heteroptera: Pentatomidae) têm sido criadas em laboratório com larvas de Tenebrio molitor L. (Coleoptera: Tenebrionidae). No entanto, não existem relatos sobre a predação, no campo ou em laboratório, de P. nigrispinus em Spodoptera frugiperda (Lepidoptera: Noctuidae), uma das principais pragas de inúmeras culturas no Brasil. Este trabalho teve o objetivo de avaliar o desenvolvimento ninfal e características reprodutivas do percevejo predador P. nigrispinus em la...

  6. Desenvolvimento do predador Podisus nigrispinus alimentado com Spodoptera frugiperda e Tenebrio molitor Development of the predator Podisus nigrispinus fed on Spodoptera frugiperda and Tenebrio molitor

    OpenAIRE

    Harley Nonato de Oliveira; Dirceu Pratissoli; Eder Pin Pedruzzi; Marcelo Curitiba Espindula

    2004-01-01

    Ninfas de Podisus nigrispinus (Heteroptera: Pentatomidae) têm sido criadas em laboratório com larvas de Tenebrio molitor L. (Coleoptera: Tenebrionidae). No entanto, não existem relatos sobre a predação, no campo ou em laboratório, de P. nigrispinus em Spodoptera frugiperda (Lepidoptera: Noctuidae), uma das principais pragas de inúmeras culturas no Brasil. Este trabalho teve o objetivo de avaliar o desenvolvimento ninfal e características reprodutivas do percevejo predador P. nigrispinus em la...

  7. Biología reproductiva y caracterización morfológica de los estadios larvarios de Hermetia illucens (L., 1758) (Diptera: Stratiomyidae). Bases para su producción masiva en Europa

    OpenAIRE

    Gobbi, Flavia Paola

    2012-01-01

    Hermetia illucens (Linnaeus, 1758) es un díptero estratiomido (Diptera, Stratiomyidae) vulgarmente denominado "mosca soldado negra" (Black Soldier Fly, BSF en inglés) de origen posiblemente neotropical pero actualmente está presente en zonas cálidas de todo el mundo, debido a su transporte accidental o a su introducción deliberada con diferentes usos. La especie es susceptible de ser criada a escala masiva y los estadios larvarios pueden alimentarse de multitud de restos orgánicos de muy dive...

  8. Análise da atividade turística desenvolvida na área de proteção ambiental dos Recifes de Corais RN

    OpenAIRE

    Silva, Clébia Bezerra da

    2009-01-01

    As unidades de conservação são criadas para proteger a natureza. A maneira como elas se relacionam com as comunidades vizinhas e com seu visitantes é determinante para que seus objetivos sejam atingidos. O presente trabalho analisa a forma como é desenvolvida a atividade turística na Área de Proteção Ambiental dos Recifes de Corais (APARC), através da identificação da imagem que moradores do distrito de Maracajaú e os turistas que visitam o parracho de Maracajaú têm com relação a este, com...

  9. Título da página electrónica: Mission de Recherche Droit et Justice

    Directory of Open Access Journals (Sweden)

    João Paulo Dias

    2003-10-01

    Full Text Available A Mission de Recherche Droit et Justice foi criada, em 1994, numa iniciativa conjunta entre o Ministério da Justiça francês e o Centre Nationale de Recherches Scientifiques. Esta associação permite a integração, numa estrutura comum, de meios humanos, intelectuais e materiais, públicos e privados, considerados necessários para o desenvolvimento de actividades científicas de investigação. Entre os seus membros fundadores com poderes deliberativos no Conselho de Administração, encontramos també...

  10. Avaliação da promoção do aleitamento materno em Hospitais Amigos da Criança

    OpenAIRE

    Mateus Freire L Souza; Priscilla Nunes Ortiz; Poliana Louzada Soares; Tatiana de Oliveira Vieira; Graciete Oliveira Vieira; Luciana Rodrigues Silva

    2011-01-01

    OBJETIVO: Avaliar o cumprimento dos Passos 4 a 10 dentre os Dez Passos para o Sucesso do Aleitamento Materno, preconizados pela Iniciativa Hospital Amigo da Criança (IHAC), criada pela Organização Mundial da Saúde (OMS). MÉTODOS: Estudo descritivo de corte transversal. Foram realizadas entrevistas com 100 puérperas nos dois hospitais credenciados pela IHAC em Salvador. Não foram incluídas mães ou recém-nascidos que não poderiam oferecer ou receber aleitamento materno exclusivo, respectivament...

  11. “La Nana”, y la evolución de una sujeción atávica al empleo remunerado

    Directory of Open Access Journals (Sweden)

    Manuel Gárate

    2009-11-01

    Full Text Available Probablemente una de las primeras reacciones después de ver La Nana, sea el rechazo ante una institución tan iberoamericana como es la criada o empleada doméstica. Institución desaparecida de la mayor parte de los hogares europeos durante la primera mitad del siglo XX a causa del encarecimiento de la mano de obra, en América Latina aún refleja el abismo que separa a las clases sociales. Al menos en Chile – la realidad que mejor conozco –  el servicio doméstico surge como una necesidad económ...

  12. A insustentável teoria da sustentabilidade: Ideologia e reificação no discurso empresarial da responsabilidade social no Brasil

    OpenAIRE

    Batista, Erika [UNESP

    2013-01-01

    Dadas as condições de exploração e esgotamento dos recursos naturais e sociais criadas pelo modo de sociabilidade capitalista contemporâneo, uma nova tentativa de reestruturação produtiva avança na direção de novas fontes de reprodução da acumulação do capital configurando um novo modelo organizacional: o da sustentabilidade. Articulado sobre três pilares – o desenvolvimento sustentável, a governança corporativa e a responsabilidade social empresarial – esta teoria organizacional está present...

  13. Insect Fauna Associated with Exposed Pig Carcasses in Southern Brazil

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    Ana Ries

    2015-12-01

    Resumo. O conhecimento dos padrões de insetos que visitam carcaças em decomposição e a análise dos parâmetros relacionados a sua presença auxilia na determinação do intervalo post-mortem (IPM. Estas informações dependem de estudos regionais, pois a diversidade de insetos e as condições ambientais interferem neste contexto. O objetivo do estudo foi analisar a entomofauna que coloniza carcaças expostas em diferentes estágios de decomposição na área de estudo. O experimento foi conduzido no sul do Brasil utilizando três porcos domésticos (Sus scrofa Linnaeus, abatidos no local. Insetos adultos associados às carcaças foram amostrados diariamente com rede entomológica, coleta manual e armadilhas pitfall. Foram realizados testes estatísticos para avaliar a diversidade de insetos. Durante o processo de decomposição (14 dias, espécimes de Scarabaeidae (25% e Calliphoridae (23% foram os mais abundantes, considerando-se a totalidade das amostras. A diversidade de insetos é distinguível quando são analisados todos os parâmetros. A fase de Putrefação negra (IV exibiu a maior diversidade de acordo com os métodos aplicados. O padrão de sucessão foi estabelecido a partir do índice de dominância das espécies: fase Fresca - Lucilia eximia Wiedemann (Diptera, Calliphoridae, fase Cromática e de Inchamento – Chrysomya albiceps (Wiedemann (Diptera, Calliphoridae, e fase de Putrefação Negra e de Esqueletização – Aphodiinae sp. 1 (Coleoptera, Scarabaeidae.

  14. Oral myiasis in an adult associated with filariasis and Hansen's disease.

    Science.gov (United States)

    Candamourty, Ramesh; Venkatachalam, Suresh; Yuvaraj, Vaithilingam; Sujee, Chembilary

    2013-01-01

    Oral myiasis is a common parasitic infestation of live human and animals caused by species of dipteran fly larvae known as maggots which may be secondary to medical disease. This case involves a 51-year-old female, poorly debilitated with advanced periodontal disease infected by the dipteral larvae in the anterior maxillary region which belonged to the family Calliphoridae and Chrysomya bezziana species. This lady was neglected from her family and presented oral myiasis with the previous history of filariasis and Hansen's disease. Secondary infestations may occur in cancrum oris, oral extraction wounds, jaw bone wounds, oral leprosy lesion, filariasis, and carcinoma. Hansen's disease (leprosy) is bacterial in origin whereas filariasis (elephantiasis) is parasitic in origin like-myiasis. The treatment consisted of manual removal of the larvae by topical application of turpentine oil, oral therapy, and surgical debridement of the oral wound.

  15. Visitation patterns of principal species of the insect-complex at carcasses in the Kruger National Park

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    L.E.O Braack

    1981-01-01

    Full Text Available Two full-grown impala rams Aepyceros melampus were shot on 1978.01.07 in the Pafuri area of the Kruger National Park, Republic of South Africa. The carcasses were placed in enclosures 2,7 km apart and used to monitor the visitation patterns of insects. Collections of insects were made at four-hourly intervals for the first six days after placement of the carcasses, and thereafter every six hours up to the eleventh and final day. A figure is given to describe changes in the physical attributes of the carcasses through time. Twelve figures depict the patterns of arrival of insects at the carrion habitat. Species from the following families are represented: Cleridae, Dermestidae, Histeridae, Scarabaeidae, Silphidae, Staphylinidae, Trogidae (Coleoptera; Calliphoridae, Muscidae, Piophilidae, Sepsidae (Diptera; Diapriidae and Formicidae (Hymenoptera. The results indicate that species have distinctive periods of abundance and presents an overall picture of insect succession at carrion.

  16. The type specimens of Calyptratae (Diptera) housed in non-traditional institutions in Argentina.

    Science.gov (United States)

    Patitucci, Luciano Damián; Mulieri, Pablo Ricardo; Domínguez, M Cecilia; Mariluis, Juan Carlos

    2015-01-14

    The type material of species of Calyptratae Diptera belonging to Anthomyiidae, Calliphoridae, Fanniidae, Muscidae, Sarcophagidae, and Tachinidae, housed in the collections of non-traditional institutions in Argentina were examined. These collections were included in the recently created "Sistema Nacional de Datos Biológicos" (National Biological Data System). We examined four collections: "Administración Nacional de Laboratorios e Institutos de Salud 'Dr. Carlos G. Malbrán'" (ANLIS), "Instituto Nacional de Tecnología Agropecuaria, Castelar, Buenos Aires" (INTA), "Instituto Argentino de Investigaciones de las Zonas Áridas" (IADIZA); and "Fundación Félix de Azara" (CFA). Comparison of the original descriptions of these species with the label information revealed the existence of 24 holotypes, 5 lectotypes, 11 syntypes, and 441 paratypes/paralectotypes. Complete information is given for each type, including reference to the original description, label data, and preservation condition. 

  17. Using Estimated On-Site Ambient Temperature Has Uncertain Benefit When Estimating Postmortem Interval

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    Laurent Dourel

    2010-01-01

    Full Text Available The forensic entomologist uses weather station data as part of the calculation when estimating the postmortem interval (PMI. To reduce the potential inaccuracies of this method caused by the distance between the crime scene and the meteorological station, temperature correlation data from the site of the corpse may be used. This experiment simulated the impact of retrospective weather data correction using linear regression between seven stations and sites in three climatic exposure groups during three different seasons as part of the accumulated degree days calculation for three necrophagous species (Diptera: Calliphoridae. No consistent benefit in the use of correlation or the original data from the meteorological stations was observed. In nine cases out of 12, the data from the weather station network limited the risk of a deviation from reality. The forensic entomologist should be cautious when using this correlation model.

  18. DNA extraction and barcode identification of development stages of forensically important flies in the Czech Republic.

    Science.gov (United States)

    Olekšáková, Tereza; Žurovcová, Martina; Klimešová, Vanda; Barták, Miroslav; Šuláková, Hana

    2017-03-21

    Several methods of DNA extraction, coupled with 'DNA barcoding' species identification, were compared using specimens from early developmental stages of forensically important flies from the Calliphoridae and Sarcophagidae families. DNA was extracted at three immature stages - eggs, the first instar larvae, and empty pupal cases (puparia) - using four different extraction methods, namely, one simple 'homemade' extraction buffer protocol and three commercial kits. The extraction conditions, including the amount of proteinase K and incubation times, were optimized. The simple extraction buffer method was successful for half of the eggs and for the first instar larval samples. The DNA Lego Kit and DEP-25 DNA Extraction Kit were useful for DNA extractions from the first instar larvae samples, and the DNA Lego Kit was also successful regarding the extraction from eggs. The QIAamp DNA mini kit was the most effective; the extraction was successful with regard to all sample types - eggs, larvae, and pupari.

  19. Blow Flies from Forest Fragments Embedded in Different Land Uses: Implications for Selecting Indicators in Forensic Entomology.

    Science.gov (United States)

    de Souza, Mirian S; Pepinelli, Mateus; de Almeida, Eduardo C; Ochoa-Quintero, Jose M; Roque, Fabio O

    2016-01-01

    Given the general expectation that forest loss can alter biodiversity patterns, we hypothesize that blow fly species abundances differ in a gradient of native vegetation cover. This study was conducted in 17 fragments across different landscapes in central Brazil. Different land cover type proportions were used to represent landscape structure. In total, 2334 specimens of nine species of Calliphoridae were collected. We used principal component analysis (PCA) to reduce dimensionality and multicollinearity of the landscape data. The first component explained 70%, and it represented a gradient of forest-pasture land uses. Alien species showed a wide distribution in different fragments with no clear relationship between the abundance values and the scores of PCA axes, whereas native species occurred only in areas with a predominance of forest cover. Our study revealed that certain native species may be sensitive to forest loss at the landscape scale, and they represent a bioindicator in forensic entomology.

  20. Multiple species of scuttle flies (Diptera: Phoridae) as contaminants in forensic entomology laboratory insect colony.

    Science.gov (United States)

    Zuha, R M; Jenarthanan, L X Q; Disney, R H L; Omar, B

    2015-09-01

    In forensic entomology, larval rearing usually includes the presence of biological contaminants including scuttle flies (Diptera: Phoridae). Scuttle flies are recognized as forensically important insects and have been reported causing nuisance and contamination in laboratory environments. This paper reports for the first time the finding of multiple scuttle fly species affecting colonies of third instar larvae of the Oriental latrine blowfly, Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae), reared indoors at the Forensic Science Simulation Site, Universiti Kebangsaan Malaysia. Adult scuttle flies were discovered inside a rearing container after the emergence of adult C. megacephala., The scuttle fly species are Megaselia scalaris (Loew), M. spiracularis Schmitz and Puliciphora borinquenensis (Wheeler). Notes on the life history and biology of these species are discussed herein.

  1. Forensic entomology and the estimation of the minimum time since death in indoor cases.

    Science.gov (United States)

    Bugelli, Valentina; Forni, David; Bassi, Luciani Alessandro; Di Paolo, Marco; Marra, Damiano; Lenzi, Scilla; Toni, Chiara; Giusiani, Mario; Domenici, Ranieri; Gherardi, Mirella; Vanin, Stefano

    2015-03-01

    Eight cases that occurred indoors in which the insects played an important role in the mPMI estimation are presented. The bodies of socially isolated people and old people living alone were discovered in central Italy between June and November. mPMI ranged from a few days to several weeks. Insects were collected during the body recovery and the postmortem. Climatic data were obtained from the closest meteorological stations and from measurements performed on the site. Sarcophagidae and Calliphoridae species were present in 75% of the cases with Lucilia sericata and Chrysomya albiceps collected in 50% of the cases. Chrysomya albiceps was always found in association with Lucilia species. Scuttle flies (Phoridae) were found in 37.5% of the cases, confirming the ability of these species in indoor body colonization. We show that if sealed environment may delay, the insect arrival dirty houses may create the environment where sarcosaprophagous insects are already present.

  2. A forensic entomology case from the Amazon rain forest of Brazil.

    Science.gov (United States)

    Pujol-Luz, José R; Marques, Helder; Ururahy-Rodrigues, Alexandre; Rafael, José Albertino; Santana, Fernando H A; Arantes, Luciano C; Constantino, Reginaldo

    2006-09-01

    The first case of application of forensic entomology in the Brazilian Amazonia is described. The corpses of 26 men were found in the rainforest in Rondonia State, Brazil. Fly larvae collected on the bodies during autopsy were identified as Paralucilia fulvinota (Diptera, Calliphoridae). No data or specimens were collected at the crime scene. At the laboratory, the larvae developed into pupae in 58 h and into adults in 110.5 h. The total development time for P. fulvinota was measured in field experiments inside the forest. The age of the larvae when collected from the bodies was estimated as the difference between the time required for them to become adults and the total development time for this species. The estimated age of the maggots and the minimum postmortem interval was 5.7 days.

  3. Volatile organic compounds released by blowfly larvae and pupae: new perspectives in forensic entomology.

    Science.gov (United States)

    Frederickx, C; Dekeirsschieter, J; Brostaux, Y; Wathelet, J-P; Verheggen, F J; Haubruge, E

    2012-06-10

    To evaluate postmortem intervals (PMIs), one should take into account the determined age of necrophagous flies present on the cadaver. However, PMI determination needs further improvement, and rapid and accurate approaches have therefore to be developed. While previous studies have focussed on insect cuticular hydrocarbons, here we explore the volatile profile released by larvae and pupae of Calliphora vicina Robineau-Desvoidy (Diptera: Calliphoridae). We monitored changes in volatile compounds daily, by headspace solid-phase microextraction, followed by gas chromatography-mass spectrometry. Branched and unbranched hydrocarbons, alcohols, esters and acids were identified, and the volatile profile was shown to vary, in both composition and quantity, with the age of the larva/pupa under investigation. We concluded, based on the analysis of the released volatile organic compounds, that it is possible to increase the accuracy of the estimated PMI, through improved estimation of the age of blowflies present on the cadaver.

  4. A review of necrophagous insects colonising human and animal cadavers in south-east Queensland, Australia.

    Science.gov (United States)

    Farrell, Julianne F; Whittington, Andrew E; Zalucki, Myron P

    2015-12-01

    A review of insects collected from decomposing human remains in south-east Queensland yielded 32 species in three orders (Diptera, Coleoptera, Hymenoptera) and 11 families (Calliphoridae, Sarcophagidae, Muscidae, Phoridae, Sepsidae, Chironomidae, Dermestidae, Cleridae, Histeridae, Staphylinidae, Encyrtidae). There were 15 cases where remains were located indoors and five cases where remains were outdoors, in both terrestrial and aquatic environments. Coleoptera were strongly associated with outdoors remains, while dipteran species composition was similar in both indoor and outdoor habitats. Some Diptera were only associated with indoors remains, while others were similarly restricted to remains recovered outdoors. Hymenopteran parasitoids were active in both habitats. Comparative collections were made from other vertebrate remains, including road-kill and farmed animals throughout south-east Queensland (Qld) and northern New South Wales (NSW) during the same period.

  5. Dipteran fauna associated with decaying organic matter in semi-deciduous forest fragments in southern Goiás, Brazil

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    José Eduardo Neto de Sousa

    2014-12-01

    Full Text Available Dipteran diversity was studied in four seasonal, semi-deciduous forest fragments in southern Goiás state, Brazil. Species richness of Muscidae, Calliphoridae and Sarcophagidae families was analyzed. The insects were collected each two months over the course of one year, using traps with decaying bait. The traps were kept in the field for five days each sampling period. A total of 1,956 individuals belonging to 13 families were collected. 29 species were identified belonging to the main families. Muscidae had the highest abundance, while Sarcophagidae presented the greatest number of species. We also report the occurrence of Philornis schildi (Muscidae for the first time in Brazil. These data reinforce the importance of preserving these areas, and indicate the need for new studies to improve knowledge of the biodiversity associated with these fragments. This information may also be useful in future studies regarding the impact of human activity on insect fauna associated with this environment.

  6. First Report of Necrophagous Insects on Human Corpses in Riyadh, Saudi Arabia.

    Science.gov (United States)

    Alajmi, R A; AlGhufaili, H; Farrukh, A; Aljohani, H; Mashaly, A M A

    2016-11-01

    Necrophagous species of insects provide useful complementary data to estimate the postmortem interval in forensic cases. Here, for the first time, we report on insect specimens collected from human corpses in Riyadh, Kingdom of Saudi Arabia. During the study, 14 beetle larvae were collected from the outdoor corpse (case report one) and five flies and seven beetles were collected from the indoor corpse (case report two). Sequencing was performed to study the mitochondrial DNA (mtDNA) as the prospective basis of an identification technique. The sequencing focused on a section of the cytochrome oxidase I encoding region of mtDNA. Two beetle species, Dermestes frischii (Kugelann) and Dermestes maculatus (De Geer) (Coleoptera: Dermestidae), and one fly species, Chrysomya albiceps (Wiedemann) (Diptera: Calliphoridae), were identified. These results will be instrumental in the implementation of a Saudi database of forensically relevant insects.

  7. Diptera Brachycera found inside the esophagus of a mummified adult male from the early XIX century, Lisbon, Portugal

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    Márcia Souto Couri

    2008-03-01

    Full Text Available Fly puparia and adult fragments of diptera muscid were found inside the esophagus of a mummified body from the early XIX century, buried inside the crypt of the Sacrament Church (Lisbon, Portugal. The identification of the material revealed a monospecific colonization by Ophyra capensis (Wiedemann (Diptera: Muscidae, a species known to invade corpses in the ammoniacal fermentation wave. This species can be found in corpses kept indoors, not available to the early waves of blowflies (Diptera: Calliphoridae. In the present case, the number of pupae and their developmental stage suggest that the female invaded the mummified corpse through the partially opened mouth and the oviposition took place directly inside the esophagus. This is the first case of O. capensis infesting internal organs of an intact corpse. The use of chemical products for the embalming process probably explains why external colonization did not occur.

  8. Insects of forensic importance from Rio Grande do Sul state in southern Brazil Insetos de importância forense do Rio Grande do Sul, sul do Brasil

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    Alex Sandro Barros de Souza

    2008-01-01

    Full Text Available The experiment was conducted throughout the year 2005, at the Universidade Federal de Pelotas campus. The objectives of the study were to analyze the decomposition of rabbit (Oryctolagus cunniculus L. with mean weight 2.67 Kg carcass and describe the interaction of insects acting on it, as well as the insect's potential use in legal medicine. We collected 5.239 insect specimens; 1.827 of them were obtained from larvae collected from carcasses and reared. The specimens were identified and 20 species were of forensic importance. The species Lucilia eximia (Wiedemann, 1819 and Chrysomya albiceps (Wiedemann, 1819 (Diptera, Calliphoridae were better indicators of post-mortem interval (PMI because they occurred in all seasons and were the first to reach the carcass. Hemilucilia semidiaphana (Rondani, 1850, H. segmentaria (Fabricius, 1805 (Diptera, Calliphoridae, Muscina stabulans (Fallén, 1817 and Synthesiomyia nudiseta (Wulp, 1883 (Diptera, Muscidae can disclose death time because they occur only in certain months of the year. Oxyletrum discicolle (Brullé, 1840 (Coleoptera, Silphidae and Dermestes maculates De Geer, 1774 (Coleoptera, Desmestidae were found in advanced stages of decomposition.Durante todas as estações do ano de 2005 foi conduzido um experimento em uma área do campus da Universidade Federal de Pelotas. O objetivo do estudo foi analisar a decomposição de carcaças de coelho (Oryctolagus cunniculus L. pesando 2,67 Kg em média e descrever como os insetos atuam na decomposição e seu possível uso na medicina-legal. Foram coletados 5.239 espécimes; 1.827 foram obtidos a partir da criação de imaturos coletados na carcaça. Foram identificadas 20 espécies com importância forense. As espécies mais propícias para serem usadas com indicadoras de intervalo post-mortem (IPM são Lucilia eximia (Wiedemann, 1819 e Chrysomya albiceps (Wiedemann, 1819 (Diptera, Calliphoridae por terem sido encontradas em todas as estações de coleta

  9. Insect succession on remains of human and animals in Shenzhen, China.

    Science.gov (United States)

    Wang, Yu; Ma, Meng-Yun; Jiang, Xin-Yu; Wang, Jiang-Feng; Li, Liang-Liang; Yin, Xiao-Jun; Wang, Min; Lai, Yue; Tao, Lu-Yang

    2017-02-01

    Most forensic entomological succession studies have been carried out using pig or rabbit carcasses; however, there have been few studies on the differences between insect succession patterns on human cadavers and on animal carcasses. In order to clarify the differences between decomposition and insect succession patterns of human cadavers and animal carcasses, one 49.5kg human cadaver, two large pig carcasses (45 and 48kg), two small pig carcasses (23 and 25kg) and two rabbit carcasses (both 1.75kg) were placed in the same field conditions in Shenzhen, China for a comparative study on August, 2013. The results indicated that: (1) The duration from fresh to skeletonization is in order of human cadaver>large pig carcasses>small pig carcasses>rabbit carcasses; (2) insect assemblages (including developmental stages) are more complex on larger carcasses, in order of human cadaver=large pig carcasses>small pig carcasses>rabbit carcasses; (3) the developmental rates of the same forensically important fly species on all carcasses are consistent; (4) all identified species of Calliphoridae can complete development of one generation on human cadaver, and both large and small pig carcasses, while on rabbit carcasses, only a subset of the Calliphoridae species can finish development of one generation; (5) beetles can generate offspring on human cadaver, and both large and small pig carcasses, while they do not generate offspring on rabbit carcasses. This study provides useful comparative data for decomposition and insect succession pattern of human cadaver with animal carcasses. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  10. Levantamento de dípteros de interesse forense em uma área de cerrado em Brasília - doi: 10.5102/ucs.v8i1.1055

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    Rodrigo César da Silva Castro

    2010-10-01

    Full Text Available A entomologia forense é a ciência que utiliza os conhecimentos sobre os insetos como ferramenta para solucionar casos em vários países. O presente estudo consistiu em analisar a frequência de coleta de famílias de dípteros de interesse forense em um fragmento de Cerrado. Foi utilizado um sistema de armadilhas suspensas a 1,5 m de altura do solo feito a partir de garrafas plásticas recicláveis, contendo isca de carne bovina em decomposição durante 26 dias. Após 60 dias de coletas, indivíduos das famílias Calliphoridae, Sarcophagidae, Muscidae e Uliididae foram identificados e a frequência de ocorrência dessas famílias foi estabelecida. Observou-se maior frequência de coleta de indivíduos das famílias Calliphoridae e Muscidae durante os primeiros 26 dias de experimento. Após esse período, as famílias Sarcophagidae e Uliididae começaram a aparecer em maior número. Dessa forma, foi possível estabelecer uma frequência de ocorrência de quatro famílias de dípteros em função de uma maior atratividade da isca em decomposição em um fragmento de Cerrado de Brasília.

  11. Repellent effect of some household products on fly attraction to cadavers.

    Science.gov (United States)

    Charabidze, Damien; Bourel, Benoit; Hedouin, Valery; Gosset, Didier

    2009-08-10

    The most common task of a forensic entomologist is to determine an accurate minimum post-mortem interval (PMI) using necrophagous fly larvae found on carrion. More often, blowflies (Diptera: Calliphoridae) are the first insects to detect the cadaver and, if the circumstances are favourable, to leave eggs on the body. However, several studies reveal that products such as gas or paint found on the cadaver induce a delay in the colonisation of the body, leading to an under-estimate of the PMI. Six common household products (gas, mosquito citronella repellent, perfume, bleach, hydrochloric acid and soda) were added to dead rats (Rattus norvegicus) in a field (Lille Forensic Institute, France). The presence of necrophagous flies was checked at regular intervals during 1 month. This experiment was repeated at the same period for four consecutive years. Results clearly showed the repellent effect of three of the six tested substances: gas (petroleum spirit), perfume and mosquito citronella repellent, which resulted in a mean delay of several days in the appearance of the first Dipteran species. Experiments were then carried out in controlled conditions in order to confirm previous observations. An olfactometer was specially designed to observe the behaviour of female Calliphora vicina (Diptera: Calliphoridae) in response to mice (Mus musculus) cadaver odour stimuli combined with household products. Dead mouse odour was a strong attractive stimulus for most of the tested individuals. Furthermore, it was noticed that the presence of mosquito citronella repellent, perfume, hydrochloric acid and paradichlorobenzene produced a significant repellent effect on female flies. All these results together confirm the repellent effect of some household products on flies and the necessity for forensic entomologists to consider this hypothesis when estimating the PMI.

  12. Isolation of poxvirus from debilitating cutaneous lesions on four immature grackles (Quiscalus sp.)

    Science.gov (United States)

    Docherty, D.E.; Long, R.I.R.; Flickinger, Edward L.; Locke, L.N.

    1991-01-01

    Poxvirus was isolated from nodules on four immature grackles (Quiscalus sp.) collected in two residential areas of Victoria, Texas. All of the birds were emaciated and had nodules on the eyelids, bill, legs, toes, and areas of the skin on the wings, neck, and ventral abdomen. These pox nodules were extensive and probably interfered with both sight and flight. The preliminary diagnosis was confirmed by virus isolation, histopathology, and electron microscopy. Poxvirus was isolated on the chorioallantoic membrane of embryonated hen's eggs and in Muscovy duck embryo fibroblast cell culture. Phaenicia calliphoridae (blowfly) larvae were found in one of the pox nodules, raising the possibility of mechanical transmission of the virus by contaminated adult blowfiles. /// Se aisl?? virus de viruela a partir de n??dulos presentes en cuatro estorninos inmaduros (Quiscalus sp.) capturados en dos ?!reas residenciales de Victoria, Texas. Las cuatro aves estaban emaciadas y presentaron n??dulos en los p?!rpados, pico, patas, dedos, y algunas ?!reas de la piel de las alas, cuello, y regi??n inferior del abdomen. Estos n??dulos producidos por viruela eran extensos y probablemente interfirieron con la visi??n y el vuelo. El diagn??stico presuntivo fue confirmado mediante aislamiento viral, histopatolog?-a, y microscop?-a electr??nica. Se aisl?? el virus de viruela en la membrana corioalantoidea de huevos embrionados de gallina y en cultivos celulares de fibroblasto de embri??n de pato. Se encontraron larvas de mosca azul (Phaenicia calliphoridae) en uno de los n??dulos de viruela, increment?!ndose as?- la posibilidad de transmisi??n mec?!nica del virus mediante moscas adultas contaminadas.

  13. Insects associated with edible mushroom Lentinula edodes (Berk. Pegler (Agaricales: Agaricaceae cultivation in Brazil Insetos associados ao cultivo do cogumelo comestível Lentinula edodes (Berk. Pegler (Agaricales: Agaricaceae no Brasil

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    Luzia Paccola Meirelles

    2002-11-01

    Full Text Available Lentinula edodes has adapted itself well to the Brazilian climate and its cultivation techniques are accessible to small and large producers. As with any other crop, the shiitake faces insect and pest attack problems, both during the mycelia colonization period in the substrate and during the basidiocarp formation. There is no report up to now of the pests that may attack in the crop in Brazil. Consequently, this study was carried out as a preliminary survey of the insects associated with shiitake cultivation both in the growing substrate and in the fruiting bodies. The samples were taken from properties situated in Londrina (PR, Rolândia (PR Nova Friburgo (RJ Sorocaba (SP and Viçosa (MG. As a result, insects from the orders Coleoptera (Bostrichidae, Staphilinidae and Dermestidae, Diptera (Calliphoridae, Himenoptera (Formicidae and Lepidoptera (Stenomatidae, were found. O cultivo de Lentinula edodes apresenta-se em crescente expansão no Brasil. Um dos problemas enfrentados pelos produtores é o ataque de insetos, tanto no substrato de crescimento do micélio, como durante o processo de formação do basidiocarpo. Este trabalho teve por objetivo fazer um levantamento preliminar dos insetos associados ao cultivo do shiitake. Deste modo, foram realizadas coletas de insetos invasores de culturas, por meio do uso de puçá e sugadores, em Londrina (PR, Rolândia (PR, Nova Friburgo (RJ, Sorocaba (SP e Viçosa (MG. Como resultado, foram encontrados insetos das ordens Coleóptera (Bostrichidae, Staphilinidae e Dermestidae, Diptera (Calliphoridae, Himenoptera (Formicidae e Lepidoptera (Stenomatidae.  

  14. Seasonal structure and dynamics of sarcosaprophagous fauna on pig carrion in a rural area of Cordoba (Argentina): their importance in forensic science.

    Science.gov (United States)

    Battán Horenstein, Moira; Rosso, Beatriz; García, M Dolores

    2012-04-10

    Four experiments, one in each season, were carried out during 2004 in a rural area of Córdoba, central Argentina. Two pigs (Sus scrofa L.), weighing approximately 8 kg each, were used in each of the four experiments. The animals were killed by a sharp blow to the head and immediately placed in an appropriate arthropod trap. One pig was placed in the shade and the other under direct sunlight. This research was conducted to determine the seasonal structure and dynamics of arthropods that constitute the sarcosaprophagous community in a given area of the central region of Argentina. The decomposition process was divided into five stages: fresh, bloated, decay, advanced decay and dry. The duration of each stage varied in different seasons, showing that the temperature and humidity are the most important variables that influence this process. A total of 51,500 adults and 36,909 immature were collected. More than 80% of the specimens collected belong to Insecta, and within this, Diptera were the most abundant order. The flies were mainly represented by adults and immature of the following species: Chrysomya albiceps (Wiedemann) (Calliphoridae), Musca domestica Linnaeus (Muscidae) and Fannia femoralis (Stein) (Fanniidae). Coleoptera were mainly represented by adults and immature of the species Creophilus maxillosus (Linnaeus) (Staphylinidae) and Dermestes maculatus (Geer) (Dermestidae). More than 50% of hymenopteran fauna collected belong to Formicidae. The trophic levels associated with the decomposition of the remains included five categories: necrophagous, parasites and predators of the necrophagous species, omnivores, opportunists and adventives. During faunal succession a strong dominance of the necrophagous species of the family Calliphoridae and Muscidae was observed, mainly during the early stages of decay process in all seasons.

  15. Qualidade da carne de frangos de corte submetidos a dietas suplementadas com crômio, criados em diferentes temperaturas ambientais - DOI: 10.4025/actascianimsci.v29i2.218 Meat quality of broiler chicks submeted the supplemented diets with chromium and reared in differents enviromental temperatures - DOI: 10.4025/actascianimsci.v29i2.218

    Directory of Open Access Journals (Sweden)

    Nívea Maria Brancaci Zeoula

    2007-11-01

    Full Text Available Foram realizados dois experimentos. O experimento um objetivou avaliar a composição da carne do peito de frangos alimentados com dietas suplementadas com crômio (0, 400, 800 e 1.200 μg Cr kg-1 complexado a levedura, criados sob diferentes condições de temperatura (quente, termoneutra e fria. O experimento dois objetivou avaliar a qualidade da carne do peito de frangos alimentados com dietas suplementadas com crômio (0 e 400 μg Cr kg-1, criados nas mesmas condições do experimento um e desossados em diferentes períodos (0, 5 e 24 horas. Os resultados mostram que as aves criadas em ambiente frio apresentaram a carne com menor porcentagem de matéria mineral e crômio e maior intensidade de vermelho, enquanto as aves criadas em temperatura termoneutra apresentaram carne com menor porcentagem de umidade, e as aves criadas em temperatura quente, carne com maior luminosidade. Já a suplementação de crômio não proporcionou qualquer efeito na composição e na qualidade de carne, enquanto que o aumento no período de desossa proporcionou um menor comprimento de sarcômero, força de cisalhamento e intensidade de amarelo, porém uma maior luminosidade.Two experiments were conducted being the objective of the first experiment to evaluate the the composition of breast meat of broiler chicks fed with diets supplemented with chromium yeast (0; 400; 800 and 1,200 μg Cr kg-1 reared in different environmental temperatures (hot, thermoneutral and cold. The second experiment aimed to evaluate the quality of breast meat of broiler chicks fed with diets supplemented with chromium yeast (0 and 400 μg Cr kg-1, reared in different environmental temperatures (hot, thermoneutral and cold and deboned in different periods (0, 5 and 24 hours. The results showed that birds reared in cold environmental temperature showed meat with lower mineral matter percentage and chromium and higher values of redness, whereas the birds reared in thermoneutral environment

  16. Especificidade alimentar: em busca de um caráter taxonômico para a diferenciação de duas espécies crípticas de cochonilhas do gênero Planococcus (Hemíptera: Pseudococcidae

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    Ana Luiza Viana de Sousa

    2012-09-01

    Full Text Available O objetivo deste trabalho foi detectar uma possível especificidade alimentar de duas espécies crípticas de cochonilhas do gênero Planococcus, refletida em seu desenvolvimento em frutos de cacaueiro, cafeeiro e citros. A cochonilha Planococcus minor (Maskell, obtida de frutos de cacau (Theobroma cacao L. cv. Comum, e Planococcus citri (Risso, de lavoura de café (Coffea arabica L. cv. Mundo Novo e de mudas de citros (Citrus sinensis L. Osbeck cv. Bahia, foram criadas em abóboras (Cucurbita maxima L., em laboratório. Rosetas com frutos de café foram mantidas sobre uma lâmina de 5 mm de ágar-água em placas de Petri, vedadas com filme plástico de polietileno. Em frutos de citros e cacau foram utilizadas gaiolas plásticas cilíndricas (1,5 cm x 3,0 cm, vedadas com voile na parte superior, as quais foram fixadas nos frutos por meio de um elástico. Os bioensaios foram conduzidos em câmaras climatizadas a 25 ± 1ºC, 70 ± 10% UR e 12 horas de fotofase, utilizando-se de 30 repetições. Para as cochonilhas provenientes de cafeeiro, o substrato café proporcionou o maior período ninfal de fêmeas (20,8 dias e maior longevidade (31,7 dias. Para fêmeas oriundas de cacau, o substrato cacau promoveu o menor período ninfal (21,1 dias e maior longevidade (25,0 dias. Para aquelas oriundas de citros, o substrato cacau promoveu o menor período ninfal (18,4 dias, e o substrato citros, a maior longevidade (32,0 dias. As maiores porcentagens de mortalidade (50% foram obtidas das ninfas oriundas de frutos de cacau e citros, criadas em café e cacau, e as menores foram das ninfas oriundas de frutos de café, independentemente do substrato em que foram criadas. A cochonilha P. minor mostra uma associação mais estreita com o cacau e, eventualmente, café, em relação ao citros, o que explicaria sua maior ocorrência em cacau. No entanto, P. citri não evidencia nenhuma especificidade para os três substratos testados.

  17. Qualidade da carne de frangos de corte submetidos a dietas suplementadas com crômio, criados em diferentes temperaturas ambientais = Meat quality of broiler chicks submeted the supplemented diets with chromium and reared in differents enviromental temperatures

    Directory of Open Access Journals (Sweden)

    Alexandre Oba

    2007-04-01

    Full Text Available Foram realizados dois experimentos. O experimento um objetivou avaliar a composição da carne do peito de frangos alimentados com dietas suplementadas com crômio (0, 400, 800 e 1.200 μg Cr kg-1 complexado a levedura, criados sob diferentes condições de temperatura (quente, termoneutra e fria. O experimento dois objetivou avaliar a qualidade da carne do peito de frangos alimentados com dietas suplementadas com crômio (0 e 400 μg Cr kg-1, criados nas mesmas condições do experimento um e desossados em diferentes períodos (0, 5 e 24 horas. Os resultados mostram que as aves criadas em ambiente frio apresentaram a carne com menor porcentagem de matéria mineral e crômio e maior intensidade de vermelho, enquanto as aves criadas em temperatura termoneutra apresentaram carne com menor porcentagem de umidade, e as aves criadas em temperatura quente, carne com maior luminosidade. Já a suplementação de crômio não proporcionou qualquer efeito na composição e na qualidade de carne, enquanto que o aumento no período de desossa proporcionou um menor comprimento de sarcômero, força de cisalhamento e intensidade de amarelo, porém uma maior luminosidade.Two experiments were conducted being the objective of the first experiment to evaluate the the composition of breast meat of broiler chicks fed with diets supplemented with chromium yeast (0; 400; 800and 1,200 μg Cr kg-1 reared in different environmental temperatures (hot, thermoneutral and cold. The second experiment aimed to evaluate the quality of breast meat of broiler chicks fed with diets supplemented with chromium yeast (0 and 400 μg Cr kg-1, reared in different environmental temperatures (hot, thermoneutral and cold and deboned in different periods (0, 5 and 24 hours. The results showed that birds reared in cold environmental temperature showed meat with lower mineral matter percentage and chromium and higher values of redness, whereas the birds reared in thermoneutral environment

  18. A interpretação ao piano no século XXI: novas técnicas e recursos

    OpenAIRE

    Fernandes, Maria João Correia

    2015-01-01

    A presente investigação pretende estudar de que forma as novas técnicas e recursos para piano, usadas por compositores do século XX e XXI, podem influenciar o trabalho do intérprete, quais as dificuldades por elas criadas e a forma como podem alterar a preparação do pianista. O projeto está organizado em duas componentes, uma teórica e uma empírica. A primeira está dividida em duas partes e começa com um enquadramento histórico sobre a música para piano desde o início do século XX até a...

  19. Peso à cobrição e ganho de peso durante a gestação de cabras nativas, exóticas e mestiças no semi-árido

    OpenAIRE

    Medeiros,Geovergue Rodrigues de; Pimenta Filho, Edgard Cavalcanti; Sousa,Wandrick Hauss de; Brito,Evaneusa de Alves

    2004-01-01

    Avaliaram-se os efeitos de mês, ano, ordem de parto e tipo de parto sobre o peso à cobrição (PC) e o ganho de peso durante a gestação (GP), respectivamente, de 753 e 527 cabras nativas Canindés, 463 e 333 cabras exóticas Anglo-nubianas, 374 e 296 Alpinas, 151 e 87 de mestiças ½ Alpina (A) ½ Sem Raça Definida (SRD), 92 e 73 ¾ Alpina (A) ¼ SRD, criadas na Estação Experimental de Pendência - EMEPA-PB, Soledade-PB, na microrregião semi-árida da Paraíba, em regime semi-intensivo, no período de 198...

  20. Alarico, chefe dos visigodos : rebelião e poder nos finais do império romano (395-410)

    OpenAIRE

    Gama, Bruno Manuel Martins

    2017-01-01

    As transformações político-militares do final do século IV e início do século V retratam o período de desagregação do Império Romano, que dominava todo o Mediterrâneo, grande parte do continente europeu, do Próximo Oriente e Norte de Africa. As instabilidades criadas pelos conflitos internos e pressões de forças externas ao limes ditariam o fim da parte ocidental do Império e o surgimento de um novo mapa europeu, de novos reinos criados pelos germanos e outros bárbaros. Neste contexto é pr...

  1. A civilização maia: contextualização historiográfica e arqueológica The Mayan Civilization: historiographic and archeological contextualization

    OpenAIRE

    Alexandre Guida Navarro

    2008-01-01

    Este artigo versa sobre a contextualização historiográfica e arqueológica da civilização maia. O intuito é traçar um perfil das primeiras explorações nos sítios arqueológicos, observar a construção do conhecimento criada acerca das primeiras informações obtidas pela Arqueologia, além das diversas vinculações das pesquisas empreendidas em relação aos contextos históricos de sua época e as teorias utilizadas atualmente pelos maístas.This article deals with the historiographic and archeological ...

  2. PROPOSIÇÃO DE UM MÉTODO PARA MENSURAÇÃO DE RANKINGS COM EMPREGO DA TÉCNICA DELPHI

    OpenAIRE

    Sidney Chaves; José Afonso Mazzon; Cesar Alexandre de Souza

    2013-01-01

    A técnica Delphi tem sido largamente empregada em estudos e pesquisas, tanto em sua forma tradicional, voltada à geração de estimativas, quanto para a elaboração de rankings. Entretanto, apesar de ser uma técnica criada há mais de 60 anos, ainda não se encontram na literatura acadêmica proposições adequadamente estruturadas e completas de sistemáticas e métodos para aplicação da Delphi em sua ranking form. Para suprir esta lacuna, propõe-se um Método para Obter e Analisar Rankings com Emprego...

  3. As várias faces de Simone: um encontro com os anseios humanos

    Directory of Open Access Journals (Sweden)

    Bernardes, Saimon

    2006-01-01

    Full Text Available Em 2002, o filme S1m0ne, de Andrew Nicoll, trouxe à tona uma nova discussão. No filme, um diretor de cinema passa a utilizar uma atriz criada pelo computador chamada Simone e que, em pouco tempo, torna- se um mito para as pessoas do mundo inteiro. Na era em que digital, virtual, clonagem e fama tornaram- se assuntos comumente falados, o filme levanta diversas possibilidades para essa discussão. Diante disso, o objetivo deste artigo é realizar uma análise do filme S1m0ne focalizando, principalmente, os aspectos: representação, digital, simulacro, mito e imaginário

  4. Título da página electrónica: Réseau d’Etude sur l’Enseignement Supérieur (RESUP

    Directory of Open Access Journals (Sweden)

    Aline Seabra Santos

    2012-10-01

    Full Text Available A RESUP, Rede de Estudo sobre o Ensino Superior, foi criada em 2001 com o objectivo de promover a investigação em sociologia, ciências políticas, ciências da educação e economia no domínio do Ensino Superior. Por três grandes motivos, a sua página, disponível apenas em francês, pode revelar-se muito útil aos investigadores interessados nas questões do Ensino Superior. Por um lado, através dela podem ser consultadas as Actas de jornadas científicas e colóquios organizados pela RESUP sobre o En...

  5. A Biblioteca da Real Academia dos Guardas-Marinha: a constituição e organização de um acervo especializado mediada pela formação profissional do oficialato militar-

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    Carlos André Lopes da Silva

    2014-07-01

    Full Text Available Em 1802, era criada uma biblioteca para prover lentes e alunos da Real Academia dos Guardas-Marinha, instituição militar de ensino que formava o oficialato empregado na Marinha portuguesa, de saberes diversos relacionados com a atividade de uma marinha de guerra. Com a vinda daquela Academia junto com a Corte, a Biblioteca dos Guardas-Marinha tornou-se a primeira biblioteca pública a funcionar no Rio de Janeiro. Contudo, é a organização temática estabelecida pelo seu primeiro diretor que demonstra o entrelaçamento entre conteúdos científicos e artes mecânicas na formação profissional do oficialato militar-naval no período joanino.

  6. Proteção ambiental e atividade minerária

    OpenAIRE

    Corrêa, Jacson

    2001-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Jurídicas, Programa de Pós-Graduação em Direito. Análise da exploração do carvão na região sul de Santa Catarina e suas consequências ambientais, a partir de estudo de caso envolvendo a defesa da Área de Proteção Ambiental criada pelo Município de Criciúma nas localidades de Morro Albino e Morro Estevão. Trata a questão ecológica sob a ótica da operacionalização prática do direito ambiental e dos princípios...

  7. The order-picking routing problem for low-level order picker in a warehouse

    OpenAIRE

    Antonio Carlos Bonassa; Claudio Barbieri da Cunha

    2011-01-01

    O presente trabalho trata do problema da determinação de rota de separação manual de peças em armazéns que minimize a distância total percorrida pelo separador. O problema abordado é prático e comum a várias empresas, com impacto nos custos operacionais e relevância para a assertividade em relação aos itens coletados. Ainda assim, o tema é pouco explorado nos estudos de roteirização disponíveis em língua portuguesa e muitas empresas optam por confiar nas rotas criadas empiricamente pelos sepa...

  8. Carta de Búzios: proposta para o controle da toxoplasmose no Brasil = Letter of Búzios: proposition for the control of toxoplasmosis in Brazil

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    Bichara, Cléa C.

    2010-01-01

    Full Text Available Nos dias 20 e 21 de setembro de 2008, em Armação dos Búzios, Rio de Janeiro, por ocasião do Congresso Centenário do Toxoplasma, foi realizado o Primeiro Simpósio Nacional de Toxoplasmose, que reuniu profissionais que se dedicam ao estudo da toxoplasmose. Foi criada a Rede Brasileira de Toxoplasmose, com finalidade de incrementar a parceria em pesquisa e a troca de experiências assistenciais, de forma a encontrar soluções mais abrangentes, que possam beneficiar o maior número possível de brasileiros. Este documento sintetiza as preocupações desse grupo de especialistas e propõe a inclusão dos itens descritos abaixo na pauta dos órgãos oficiais

  9. Cheese yield, casein fractions and major components of milk of Saanen and Anglo-Nubian dairy goats Rendimento de produção de queijos, frações de caseína e principais componentes do leite de cabras Saanen e Anglo-Nubiana

    OpenAIRE

    J. P. Damián; Sacchi,I.; S. Reginensi; D. de Lima; Bermúdez, J.

    2008-01-01

    Avaliaram-se os componentes, as frações de caseína e o rendimento na produção de queijo do leite de cabras das raças Saanen e Anglo-Nubiana, principais raças criadas no Uruguai. O estudo foi realizado em uma fazenda com sistema de criação semi-intensiva. O leite das cabras Anglo-Nubianas apresentou teores mais elevados de gordura (4,65 vs 3,59%), proteína total (3,48 vs 2,84%), caseína total (2,82 vs 2,23%), e maior rendimento na produção de queijos (22,00 vs 15,03kg/100l) que o leite das cab...

  10. Cheese yield, casein fractions and major components of milk of Saanen and Anglo-Nubian dairy goats

    OpenAIRE

    J. P. Damián; Sacchi,I.; S. Reginensi; D. de Lima; Bermúdez, J.

    2008-01-01

    Avaliaram-se os componentes, as frações de caseína e o rendimento na produção de queijo do leite de cabras das raças Saanen e Anglo-Nubiana, principais raças criadas no Uruguai. O estudo foi realizado em uma fazenda com sistema de criação semi-intensiva. O leite das cabras Anglo-Nubianas apresentou teores mais elevados de gordura (4,65 vs 3,59%), proteína total (3,48 vs 2,84%), caseína total (2,82 vs 2,23%), e maior rendimento na produção de queijos (22,00 vs 15,03kg/100l) que o leite das cab...

  11. Educação e desenvolvimento em Pernambuco entre 2004 e 2014: desvelando os nexos do programa de educação integral com o rejuvenescimento da teoria do capital humano

    OpenAIRE

    SANTIAGO, Frederico Marcio Leandro

    2014-01-01

    Esta pesquisa aborda a relação entre educação e desenvolvimento no Programa de Educação Integral de Pernambuco (PEI). O Programa de Educação Integral foi instituído pela Lei Complementar nº 125/2008 e se constitui como principal estratégia do governo de Eduardo Campos para requalificar o Ensino Médio na rede pública estadual de educação se materializando através das Escolas de Referência no Ensino Médio (Erem), criadas pelo Decreto Estadual nº 34.607/2010. Buscamos desvelar os nexos presentes...

  12. Mineração de dados para inferência de relações solo-paisagem em mapeamentos digitais de solo.

    OpenAIRE

    CRIVELENTI, R. C.; COELHO, R. M.; ADAMI, S. F.; OLIVEIRA, S. R. M.

    2010-01-01

    O objetivo deste trabalho foi desenvolver uma metodologia para mapeamento digital de solos na escala 1:100.000 com a aplicação de técnicas de mineração de dados a descritores de relevo e a dados de mapas geológico e pedológico preexistentes. Foi criada uma base de dados digitais a partir de cartas topográficas e temáticas, que permitiu elaboração do modelo digital de elevação (MDE) da folha Dois Córregos, SP (escala 1:50.000). A partir do MDE, foram calculados os parâmetros geomorfométricos d...

  13. REPRESENTAÇÕES DO FEMININO EM THE TURN OF THE SCREW: A PRECEPTORA COMO ANJO E MONSTRO

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    LINDA CATARINA GUALDA

    2007-01-01

    Full Text Available Este artigo analisa como se constrói a representação feminina em The Turn of the Screw, tendo como ponto de partida a preceptora. O ideal de mulher apresentado é uma criação masculina e faz referência a uma construção social que tem a ver com a distinção masculino/feminino, colocando a mulher numa posição de inferioridade e veiculando uma imagem negativa dessa mulher. Ointeresse do estudo é esse estereótipo de mulher que se constrói a partir dos mitos concebidos e se incorpora ao gênero dando-lhe um caráter de naturalidade. À luz da linha feminista da crítica literária, pretendemos refletir a respeito da identidade de gênero criada e veiculada sob uma ótica masculina.

  14. O leitor e as armadilhas do texto nos contos: ‘O Evangelho Segundo Marcos’ de Jorge Luis Borges e ‘Via Crucis’ de Clarice Lispector

    OpenAIRE

    Schmidt, Marina Kione

    2012-01-01

    O presente artigo consiste em  desenvolver uma análise para identificar e interpretar o modelo de leitor, conferindo a ele um espaço de movimentação para que se estabeleça um diálogo entre os contos O Evangelho segundo Marcos de Jorge Luis Borges e Via Crucis  de Clarice Lispector. Identificaremos aqui o processo de fragmentação e construção das armadilhas  criadas pelos  narradores  no decorrer das  tramas,  acompanhando a trajetória de cada  narrador e personagens, apontado  os artifícios t...

  15. Avaliação clínica de pacientes com distonia idiopática

    OpenAIRE

    Torben Cavalcante Bezerra

    2016-01-01

    INTRODUÇÃO: A distonia é caracterizada por contrações musculares sustentadas, frequentemente gerando movimentos de torção, repetitivos ou posturas anormais. A classificação de distonia é baseada em três eixos: idade de início, distribuição e etiologia. A definição criada pelo Comitê da Fundação de Pesquisa Médica em Distonia, em 1984, ainda está em uso atualmente. Questionamentos têm sido feitos para uma nova proposta de classificação de distonia. MATERIAIS E MÉTODOS: Trata-se de um estudo tr...

  16. Televisão, violência e efeitos midiáticos

    Directory of Open Access Journals (Sweden)

    Mériti de Souza

    Full Text Available No mundo contemporâneo, observamos a restrição das relações interpessoais em decorrência da violência nas grandes cidades, do estímulo ao convívio restrito à família e ao par amoroso e da produção da subjetividade individualizada. A ilusão criada pela televisão de que o telespectador participa das situações reproduzidas na tela contribui para com essa restrição, produzindo o espaço público mediatitizado. Dentre as implicações desse processo, trabalhamos com a idéia de o discurso televisivo assumir a função de porta-voz do telespectador, veiculando a violência secundária, ao estabelecer significados para experiências virtuais do sujeito.

  17. Os cursos de enfermagem da cruz vermelha brasileira e o início da enfermagem profissional no Brasil

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    Maria Lúcia Mott

    2002-10-01

    Full Text Available O artigo discute as origens da enfermagem profissional no Brasil e o papel das escolas de enfermagem da Cruz Vermelha Brasileira, criadas no Rio de Janeiro, no período da década de 1910. A pesquisa baseia-se na análise de diferentes tipos de documentos como artigos, manuais de enfermagem, estatutos, relatórios, entre outros. Aponta para a necessidade de se repensar afirmações cristalizadas na bibliografia da História da Enfermagem Brasileira, com relação à vinculação entre a chegada ao Brasil das enfermeiras da Missão Rockefeller e a profissionalização da enfermagem no país e o papel de precursora na formação de enfermeiras atribuído à Escola de Enfermeiras Dona Anna Nery, fundada no Rio de Janeiro, em 1923.

  18. EDITORIAL: Ciberpajé

    OpenAIRE

    2016-01-01

    A arte criada exclusivamente como capa para essa edição da revista Húmus é uma reflexão poética e crítica sobre a complexa relação humana com o meio ambiente do qual depende, relação baseada atualmente em uma degradante aceleração do consumismo e da obsolescência programada dos produtos. Ela foi intitulada de “Gaia e o paradoxo humano”. A figura feminina enraizada na terra simboliza Gaia, ela tem em seu ventre um signo da morte e em suas mãos um signo da vida. Mesmo entristecida continua dadi...

  19. A reconfiguração do sindicalismo brasileiro nos anos 2000: as bases sociais e o perfil político-ideológico da Conlutas

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    Patrícia Vieira Trópia

    2013-06-01

    Full Text Available Este artigo tem como objetivo contribuir para a compreensão do perfil socioeconômico e político-ideológico das bases sociais da Conlutas, central sindical e popular criada em 2004 a partir de uma dissidência da CUT, e que conta com a participação de diferentes movimentos sociais. A análise tem como base um survey realizado durante o 1º Congresso Nacional da Conlutas, ocorrido em julho de 2008 na cidade de Betim (MG. Os dados mostram que a Conlutas representa, em sua maioria, trabalhadores não manuais, do setor público, especialmente da educação, que recebem até 3 salários mínimos e que têm uma situação de trabalho caracterizada pela estabilidade.

  20. Participação pública, Comunicação e inclusão Digital

    OpenAIRE

    Guzzi, Adriana de Araujo

    2006-01-01

    o termo participação pública é utilizado em programas, projetos e iniciativas de governos de Estado democráticos. o debate em tomo do que seja a participação não é novo, mas com o advento e a propagação das tecnologias de comunicação, sobretudo a Internet, novas possibilidades democráticas foram criadas, já que os mecanismos inaugurados têm a capacidade de mudar a maneira pela qual as consultas à opinião pública, e conseqüentemente a escuta a essas consultas, podem ser trata...

  1. Análise do setor bancário : a presença dos bancos espanhóis no Brasil

    OpenAIRE

    2010-01-01

    Apresenta as razões que levaram bancos espanhóis a investir no setor bancário brasileiro e analisa os resultados dessa estratégia. Discute a relação entre o processo de consolidação mundial do setor, as estratégias utilizadas pelos bancos espanhóis e os efeitos no sistema bancário brasileiro. Descreve a formação do setor bancário no Brasil e sua consolidação a partir do Plano Real. Analisa a internacionalização dos bancos espanhóis e a opção pela América Latina. Discute as expectativas criada...

  2. Eficacia del Fipronil en el control del ciclo peridomiciliario de Triatoma infestans en un área con resistencia a la Deltametrina

    Directory of Open Access Journals (Sweden)

    Gentile Alberto G.

    2004-01-01

    Full Text Available Se investigó la eficacia del Fipronil líquido al 1,0% contra T. infestans, administrado pour on en aves y mamíferos, tanto en condiciones de laboratorio como en el terreno. En laboratorio se trataron con Fipronil una gallina, un perro y un caprino y se alimentaron sobre ellos ninfas III resistentes a Deltametrina. Como grupo control se utilizaron ninfas del mismo estadio criadas en laboratorio. Durante la fase de terreno se impregnaron con Fipronil 4 perros, 1 gato y 2 gallinas cluecas. La infestación intradomiciliaria fue tratada mediante Aerotermia. En laboratorio la mortalidad ninfaria fue del 100,0% a los 7 días y del 88,8% a los 30 días. En terreno, la disminución del Índice de Densidad Triatomínea - medida a los 30 días - fue del 65,4%.

  3. Em prol do sacrifício do isolamento: lepra e filantropia na Argentina e no Brasil, 1930-1946

    Directory of Open Access Journals (Sweden)

    José Augusto Leandro

    2013-09-01

    Full Text Available O Patronato de Leprosos, na Argentina, e a Federação das Sociedades de Assistência aos Lázaros e Defesa Contra a Lepra, no Brasil, foram instituições criadas com o objetivo de auxiliar doentes do mal de lázaro e suas famílias. Capitaneadas por mulheres da elite, as entidades, a despeito das singularidades dos seus contextos nacionais, agiram de maneira similar, ambas complementando as políticas de saúde direcionadas à lepra em seus respectivos países. Este artigo pretende demonstrar as semelhanças nas estratégias de atuação das duas instituições filantrópicas que, durante as décadas de 1930 e 1940, agiram em consonância com os médicos defensores do isolamento compulsório.

  4. FRACASSO E MÍSTICA – A PROPÓSITO DE CIORAN, E. M. Lacrimi și sfinți. Bucareste: Editura Humanitas, 1992.

    Directory of Open Access Journals (Sweden)

    Roberto Antônio Câmara Zarco

    2013-08-01

    Full Text Available O presente artigo detém como objetivo central, o expor da imagem/conceito do “místico fracassado” composta pelo contemporâneo filósofo/pensador romeno E. M. Cioran. Para este fim, utilizar-se-á como eixo bibliográfico central a obra Lacrimi și sfinți [Lágrimas e santos] de Cioran. No mérito da instrumentação metodológica, servir-se-á principalmente de contraposição da imagem/conceito do “místico bem-sucedido”, conforme essa é esboçada na Tradição medieval, ocidental e cristã, com a do “místico fracassado”, haja vista a primeira ser de forma íntima conectada ao modo como a segunda é criada por Cioran.

  5. Gastronomia Molecular: Desconstruindo 20 anos de Uma Tendência

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    Joana Pellerano

    2013-06-01

    Full Text Available A gastronomia molecular gera interesse e controvérsia desde que foi criada, em 1992, nos workshops de Erice, na Itália. Da curiosidade dos cientistas Nicholas Kurti e Hervé This, que desenvolveram a disciplina, à aplicação prática encontrada por chefs de todo o mundo, a gastronomia molecular é perseguida por parecer ser mais complicada do que realmente é: o estudo meticuloso dos alimentos. O objetivo desse artigo é descrever o seu surgimento e o que trouxe ao cenário contemporâneo enquanto tendência gastronômica. Para tanto, foi realizada uma revisão bibliográfica em livros e citações na imprensa estrangeira, o que permitiu descrever os motivos para que essa forma de cozinhar cause tanta polêmica.

  6. Realidades e controvérsias em relação ao conceito psicanalítico de autismo normal

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    Maria Izabel Tafuri

    2003-12-01

    Full Text Available Neste trabalho discute-se a evolução do pensamento psicanalítico a partir da noção de autismo normal e sua posterior refutação, feita por Tustin, em 1991. A proposta de refutação pode ser vista como perpetuação de outro erro: a imprecisão conceitual criada por Mahler desde a definição original do termo. Tustin restringiu, à moda de Mahler, a noção de satisfação autística do lactente na “casca do ovo” à de autismo normal.Dessa forma, a refutação do autismo normal recai na mesma problemáticagerada por Bleuler e Kanner: a subtração de Eros do conceito de auto-erotismo.

  7. Participação, deliberação e comunicação pública: o caso do orçamento participativo de Lisboa

    OpenAIRE

    Correia, Alan Albuquerque Ribeiro

    2012-01-01

    Dissertação apresentada para cumprimento dos requisitos necessários à obtenção do grau de Mestre em Ciências da Comunicação, variante de Comunicação Estratégica O Orçamento Participativo de Lisboa é uma política pública inovadora criada em 2008. A iniciativa tem o objetivo de convocar a população para escolher a maneira como será empregado um determinado percentual do orçamento municipal. Numa lógica de participação direta, os cidadãos devem propor um projeto e, caso ele seja viável tecnic...

  8. Educação a distância: avanços históricos e legislação brasileira

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    Sander Neves

    2013-06-01

    Full Text Available O presente artigo retrata a história da educação à distância no mundo, bem como o advento da internet e sua relação com a educação. Através dessa nova ferramenta tecnológica, foram criadas outras metodologias tecnológicas que aproximaram ainda mais do ensino e aprendizagem proporcionando a abertura de novos cursos à distância. O artigo também aborda as leis que regulamentam e definem o ensino a distância no Brasil, discutindo suas contribuições para o desenvolvimento e a qualidade dessa nova modalidade educacional.  

  9. A Ignorância Letrada: ensaio sobre a mediocrização do ambiente acadêmico

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    Paulo Roberto Almeida

    2010-08-01

    Full Text Available Ensaio sobre a crescente deterioração da qualidade da produção acadêmica brasileira na área de humanas, examinando a natureza do problema, suas causas, suas consequências mais evidentes e as evidências disponíveis, de modo indireto. Ademais das pedagogias freireanas, que estão na raiz da tragédia educacional nos dois primeiros ciclos de ensino, o sistema de educação superior padece de viseiras mentais criadas por ideologias supostamente progressistas que deformam a percepção da realidade e contribuem para a erosão de qualidade nos diferentes ciclos de ensino.

  10. “Una flor al tiempo dedicada”: la voz de Celia en la poética de Sor Juana Inés de la Cruz

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    Leonardo Sancho Dobles

    2015-12-01

    Full Text Available Este artículo analiza la actitud dialógica que la comedia Los empeños de una casa de Sor Juana Inés de la Cruz establece con los propios poemas de la escritora. Como referencia particular se hace énfasis en la voz de la criada Celia en la comedia y en los sonetos dedicados al motivo de la rosa para, finalmente, establecer algunas ideas con respecto a la poética de la poeta novohispana. This work analyzes the way in which the comedy House of Desires by Sor Juana Inés de la Cruz establishes a dialogic attitude with other poems of the writer. As a particular reference, I will emphasize the voice of the maid, Celia, in the comedy, as well as in the sonnets dedicated to the ‘rose’ motif, to establish, finally, some ideas on the poetics of the novohispanic poet.

  11. Humanidades e medicina: razão e sensibilidade na formação médica

    OpenAIRE

    Rios,Izabel Cristina

    2010-01-01

    No presente artigo, discute-se brevemente o ensino de humanidades na medicina e apresenta-se uma proposta criada no Centro de Desenvolvimento da Educação Médica (CEDEM) da Faculdade de Medicina da Universidade de São Paulo (FMUSP) para a integração dessa área no currículo médico. A integração curricular é uma estratégia de ensino fundamentada na visão sistêmica que envolve ações de amplo alcance educacional. No ensino de humanidades na medicina, são ações que visam à sensibilização das pessoa...

  12. Humanities and medicine: reason and sensibility in the medical education

    OpenAIRE

    Izabel Cristina Rios

    2010-01-01

    No presente artigo, discute-se brevemente o ensino de humanidades na medicina e apresenta-se uma proposta criada no Centro de Desenvolvimento da Educação Médica (CEDEM) da Faculdade de Medicina da Universidade de São Paulo (FMUSP) para a integração dessa área no currículo médico. A integração curricular é uma estratégia de ensino fundamentada na visão sistêmica que envolve ações de amplo alcance educacional. No ensino de humanidades na medicina, são ações que visam à sensibilização das pessoa...

  13. Revolução Farroupilha e discurso historiográfico: os escritos de Carlos Dante de Moraes

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    Francisco das Neves Alves

    2007-04-01

    Full Text Available A construção de discursos historiográficos a respeito da Revolução Farroupilha receberia um impulso sem precedentes a partir da virada dos anos vinte para os trinta do século XX. Progressivamente, essa guerra civil viria a ser transformada no mais importante acontecimento da história gaúcha e seria criada uma série de pressupostos que se transformariam em verdadeiros axiomas da historiografia gaúcha, através da formação de uma rede discursiva. Essa construção historiográfica se estenderia por décadas e seus efeitos se fazem sentir até o presente. O objetivo deste trabalho é analisar os escritos de Carlos Dante de Moraes que muito a contento se inseriram no discurso historiográfico predominante acerca da Revolução Farroupilha.

  14. Efeito da nicarbazina sobre o desempenho de frangos de corte recebendo diferentes níveis de lisina submetidos a estresse por calor

    OpenAIRE

    Donzele Juarez Lopez; Oliveira Rita Flávia Miranda de; Cella Paulo Cegatto; Oliveira Neto Adhemar Rodrigues de

    2001-01-01

    Este trabalho foi realizado para avaliar o efeito da adição de nicarbazina e níveis de lisina na ração de frangos de corte no período de 1 a 21 dias de idade e seu efeito residual sobre o período de 22 a 49 dias de idade. As aves foram criadas em ambiente de alta temperatura. Foram utilizados 480 frangos de corte machos da marca Ross, em delineamento inteiramente casualizado em esquema fatorial 2x4 (adição ou não de nicarbazina e nível de lisina) seis repetições e 10 aves por unidade experime...

  15. Efeito da nicarbazina sobre o desempenho de frangos de corte recebendo diferentes níveis de lisina submetidos a estresse por calor Effect of nicarbazin on the performance of broiler chicks fed diets with different lysine levels submitted to heat stress

    OpenAIRE

    Juarez Lopez Donzele; Rita Flávia Miranda de Oliveira; Paulo Cegatto Cella; Adhemar Rodrigues de Oliveira Neto

    2001-01-01

    Este trabalho foi realizado para avaliar o efeito da adição de nicarbazina e níveis de lisina na ração de frangos de corte no período de 1 a 21 dias de idade e seu efeito residual sobre o período de 22 a 49 dias de idade. As aves foram criadas em ambiente de alta temperatura. Foram utilizados 480 frangos de corte machos da marca Ross, em delineamento inteiramente casualizado em esquema fatorial 2x4 (adição ou não de nicarbazina e nível de lisina) seis repetições e 10 aves por unidade experime...

  16. Acordos de conservação da Reserva Biológica do Lago Piratuba

    OpenAIRE

    2015-01-01

    A Reserva Biológica do Lago Piratuba foi criada em 1980 como uma área isenta de ocupações humanas e sem uso de seus recursos naturais. No entanto, já existiam populações tradicionais que ocupavam e utilizavam a área, em vários pontos no interior dos limites propostos. Diante dos conflitos de uso e ocupação em razão da categoria da unidade de conservação, foram estabelecidas normas e ações específicas para compatibilizar a presença das populações tradicionais residentes com os objetivos da res...

  17. O campo da sexologia no Brasil: constituição e institucionalização

    OpenAIRE

    Jane A. Russo; Rohden,Fabíola; Torres,Igor; Faro,Livi

    2009-01-01

    Neste artigo apresentamos e discutimos os resultados parciais da investigação sobre a constituição do campo da sexologia contemporânea no Brasil, parte da pesquisa comparativa "Sexualidade, ciência e profissão na América Latina". Uma segunda onda sexológica surgiu no Brasil em finais dos anos 1970 no Rio de Janeiro, com um viés essencialmente clínico, e em São Paulo, com um viés educacional. A década seguinte marcou um período de institucionalização no qual são criadas as duas associações que...

  18. perspetiva comparativa com o sistema ProTaper®

    OpenAIRE

    Martins, Filipe Augusto Moreno Cornélio do Carmo

    2012-01-01

    Trabalho apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Medicina Dentária A Endodontia é uma área da Medicina Dentária que tem evoluído muito ao longo dos tempos. Surgiu no século XVII e desde aí, os conceitos, as técnicas e os materiais têm apresentado uma constante evolução e desenvolvimento. Alguns desses exemplos são as novas ligas metálicas que têm sido criadas e investigadas; os novos métodos de potenciação da irrigação; e as n...

  19. Eficacia del Fipronil en el control del ciclo peridomiciliario de Triatoma infestans en un área con resistencia a la Deltametrina

    Directory of Open Access Journals (Sweden)

    Alberto G. Gentile

    2004-10-01

    Full Text Available Se investigó la eficacia del Fipronil líquido al 1,0% contra T. infestans, administrado pour on en aves y mamíferos, tanto en condiciones de laboratorio como en el terreno. En laboratorio se trataron con Fipronil una gallina, un perro y un caprino y se alimentaron sobre ellos ninfas III resistentes a Deltametrina. Como grupo control se utilizaron ninfas del mismo estadio criadas en laboratorio. Durante la fase de terreno se impregnaron con Fipronil 4 perros, 1 gato y 2 gallinas cluecas. La infestación intradomiciliaria fue tratada mediante Aerotermia. En laboratorio la mortalidad ninfaria fue del 100,0% a los 7 días y del 88,8% a los 30 días. En terreno, la disminución del Índice de Densidad Triatomínea - medida a los 30 días - fue del 65,4%.

  20. The use of centrifugal pendulums for torsional vibration control in automotive powertrains = : O uso de pêndulos centrífugos para controle de vibrações torcionais em trens de potência automotivos

    OpenAIRE

    Vinícius Gabriel Segala Simionatto

    2015-01-01

    Resumo: Absorvedores de Vibração Pendulares Centrífugos (ou CPVAs) são uma tecnologia criada em 1911, que trouxe excelentes resultados na redução de vibração torcional de muitos sistemas. Porém, sua aplicação em trens de potência automotivos para redução de vibração no virabrequim tornou-se inviável ao final da década de 1940 pela existência de dampers viscosos à base de silicone, uma solução mais barata e com performance similar em certas aplicações. Contudo, a vibração torcional transmitida...

  1. Margaret Atwood: la sirena de géneros

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    Freire, Espido

    2009-06-01

    Full Text Available En la narrativa de Margaret Atwood la sorpresa, el uso poético del lenguaje y la reconstrucción constante de la identidad de los personajes guían al lector a traves de los distintos textos. Tanto en la reconstrucción de Los diarios de Susanna Moodie como en la traducción de emociones y sentimientos de la reina de Dinamarca en «La respuesta de Getrudis», la figura de las protagonistas femeninas fuertes e imprevisibles reinventan el misterio del eterno femenino. Las criadas y las señoras, desde sus peculiares puntos de vista, definen la sociedad con distancia y escepticismo, sabedoras de que son más de lo que el otro (el lector o los personajes pueden atisbar. En un acto lúdico constante, la literatura se convierte en un juego eterno, con las palabras como reglas.

  2. Título da página electrónica: Social Movements International Network/Red Internacional de los Movimientos Sociales

    OpenAIRE

    José Manuel Mendes

    2004-01-01

    No âmbito do Fórum Social Mundial, foi criada a Rede Internacional de Movimentos Sociais, com sede em São Paulo. Esta é a página dessa Rede, que congrega um vasto conjunto de movimentos sociais de diferentes países que têm como objectivo principal a luta contra a globalização neoliberal e todas as formas de opressão e exploração a nível mundial. A página apresenta-se em edição bilingue, em inglês e espanhol, e abre com as conclusões dos encontros internacionais realizados e com a agenda da Re...

  3. Representações sobre a política de institucionalização de crianças e adolescentes na perspectiva de funcionários e moradores do entorno das casas de acolhimento, Viçosa-MG: uma discussão sobre a convivência familiar e comunitária

    OpenAIRE

    Lopes, Jaqueline de Freitas

    2014-01-01

    O ponto central em que se assenta essa pesquisa é a institucionalização de crianças e adolescentes, uma das questões polêmicas dos estudos relacionados à família, uma vez que a existência de crianças e adolescentes em um contexto de abandono, miséria e violência extrema reflete uma das faces do processo de exclusão social vivenciado pela população brasileira. Diante desse abandono familiar e social, foram criadas, como medida de proteção, as casas de acolhimento institucional, nas quais são a...

  4. As missões de paz da ONU e a questão de Timor Leste : ponto de inflexão?

    OpenAIRE

    2006-01-01

    A Organização das Nações Unidas (ONU) foi criada com o objetivo precípuo de assegurar e promover a paz mundial. Em mais de 60 anos de existência, a Organização ainda não logrou implementar a força militar permanente (ver artigo 47, parágrafo 3º da Carta da ONU) que seria a principal encarregada pela consecução desse objetivo por intermédio da coordenação da Comissão de Estado Maior. O fato de não ter constituído a referida força não significou a paralisação da ONU nos assuntos concernentes à ...

  5. As Missões e Paz da ONU: a evolução da face mais visível de atuação de um organismo internacional

    OpenAIRE

    2011-01-01

    A Organização das Nações Unidas (ONU) foi criada com o objetivo precípuo de assegurar e promover a paz mundial. Em mais de 60 anos de existência, a Organização ainda não logrou implementar uma força militar permanente (ver artigo 47, parágrafo 3º da Carta da ONU) que seria a principal encarregada pela consecução desse objetivo por intermédio da coordenação da Comissão de Estado Maior. As missões de paz da ONU são a face mais visível do trabalho da Organização na promoção da paz mundial. Este ...

  6. Desculpe, não ouvi!

    Directory of Open Access Journals (Sweden)

    Comissão Editorial

    2017-01-01

    Full Text Available Carioca, criada em São Paulo e formada em Comunicação Social pela Universidade Anhembi Morumbi, a autora do livro Desculpe, não ouvi! é surda desde os 10 anos de idade. ( ... "o aprendizado mais importante com a perda auditiva foi aprender a observar o outro. Quando você se torna diferente por alguma razão, o mundo passa a te olhar de outra maneira e você se torna mais observador. Observa como o outro vai te receber antes de você se apresentar. Observa o ambiente antes de chamar atenção para si. Todos esses aprendizados, por mais dolorosos que tenham sido, me transformaram na pessoa que sou hoje e, apesar do quão difícil foi, sou grata pela oportunidade que tive de aprender tudo isso"

  7. Acreditação e qualidade da educação superior : abrindo a caixa-preta do sistema de acreditação de cursos superiores do Mercosul

    OpenAIRE

    Serikawa, Leonardo Kazuo dos Santos

    2014-01-01

    Criada em 2009, a Rede de Agências Nacionais de Acreditação (RANA) tem como função aprofundar a integração do MERCOSUL por meio do Sistema de Acreditação de Cursos Superiores (ARCU-SUL). Ante a importância dessa rede, o objetivo principal desta dissertação é conhecer o papel desempenhado por ela no reconhecimento da qualidade da educação superior no MERCOSUL. Entre seus alvos estão estabelecer critérios regionais de qualidade de cursos de graduação para a melhoria permanente da formação super...

  8. O Riso Crispado: O Macarrônico Alemão de A Manha e a Ascensão e Queda do Nazismo

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    Ana Carina Baron Engerrof

    2002-12-01

    Full Text Available Este ensaio contempla a produção de A Manha feita por personagens alemãs, criadas, ao que tudo indica, por Aparício Torelly. A discussão tem como um dos objetivos analisar maneiras pelas quais interpretações do “ser” alemão no Brasil daquele momento são efetivadas. Para tanto, partindo de uma análise rápida e parcial da produção atribuída a “colaboradores” alemães do periódico, serão discutidos originais que se referem a um único tema: o nazismo.

  9. UTILIZAÇÃO DE MÉTODOS ÁGEIS E SUAS IMPLICAÇÕES

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    Deullam Justi dos Santos

    2017-01-01

    Full Text Available Após a crise de software que ocorreu na década de 70, durante a qual nasceu a engenharia de software, pois os sistemas da época estavam com problemas para serem terminados, estourando prazos e orçamentos, e quando eram terminados tinham uma baixa qualidade com bugs e difícil manutenção. Por conta disso,foram criadas metodologias para o desenvolvimento dos sistemas.Este artigo apresenta 17 métodos ágeis como Scrum, Extreme programing, Lean, que foram utilizados por autores, Foi feita uma pesquisa bibliográfica em artigos onde foi feito uma tabela contendo os autores, metodologias utilizadas e quais os resultados de sua utilização, e gráficos de comparações das metodologias ágeis.

  10. Impacto da temperatura e fotoperíodo no desenvolvimento ovariano e oviposição de traça-das-crucíferas Impact of temperature and photoperiod on ovarian development and oviposition of the Diamondback Moth

    Directory of Open Access Journals (Sweden)

    Allan Crema

    2004-06-01

    Full Text Available A migração é um fenômeno que pode ocorrer em algumas espécies de insetos e ela geralmente começa quando os ovários não estão desenvolvidos, ou o estão parcialmente. Para traça-das-crucíferas, Plutella xylostella (Lepidoptera: Yponomeutidae já foi observado que a densidade larval afetou o grau de desenvolvimento ovariano. Neste trabalho foi determinado o impacto da temperatura e do fotoperíodo no desenvolovimento ovariano e na oviposição do inseto. Larvas de quarto estágio foram criadas individualmente em placas de Petri à temperaturas de 14 e 25ºC e fotofase de 8 e 14 h. As fêmeas recém-emergidas foram mortas em um freezer, dissecadas sob um microscópio e o ovário observado. Para determinar o impacto da temperatura sobre a oviposição do inseto, larvas de quarto estágio foram criadas a temperaturas de 14 e 25ºC e, quando os machos e fêmeas emergiram, casais foram colocados individualmente em placas de Petri. Foi determinado o período de pré-oviposição e o número de ovos depositados no primeiro dia. Os resultados mostraram que o grau de desenvolvimento ovariano não foi afetado pela temperatura e fotoperíodo empregados, indicando que estes fatores não afetaram o potencial migratório da espécie. Fêmeas criadas a 25ºC depositaram seus ovos dois dias após a emergência enquanto que fêmeas criadas a 14ºC o fizeram aos cinco dias, sugerindo que o período de pré-oviposição é aumentado a baixas temperaturas. O impacto destes resultados sobre a migração e dispersão da TDC são discutidos.Migration occurs in some insect species, and in many cases it commences when the ovaries are immature. Environmental conditions can affect the rate of ovarian development and oviposition of insects. In the Diamondback Moth (DBM, Plutella xylostella (Lepidoptera: Yponomeutidae, the larval density affected the rate of ovarian development. In this work we determined the impact of temperatures and photoperiods on DBM ovarian

  11. Pedro e Jorge Reinel (at.1504-60)

    OpenAIRE

    Moreira, Rafael

    2015-01-01

    Na côrte dos reis D. João II e D. Manuel I de Portugal, ponto de encontro de gentes de todas as raças e proveniências, os escravos negros da Senegâmbia e Guiné, batizados, instruídos e casados com criadas mestiças ou até brancas, formavam uma elite, em que os mais aptos poderiam especializar-se em variados ofícios, de criados da alta nobreza a músicos e artistas. De 1470-80 até c.1540, distinguiu-se um grupo de habilíssimos entalhadores de marfim da Serra Leoa, criadores da arte híbrida chama...

  12. A elite carioca e sua pobreza envergonhada

    Directory of Open Access Journals (Sweden)

    Ana Vasconcellos Freitas

    2014-06-01

    Full Text Available Em 1938 foi criada na cidade do Rio de Janeiro a instituição de caridade católica Apoio Fraternal, com a missão de amparar exclusivamente pessoas que tinham perdido fortuna e posição social a ponto de necessitarem da ajuda alheia. Tal amparo de caráter absolutamente sigiloso previa um complexo auxílio material e religioso. Este artigo pretende abordar a existência de uma pobreza envergonhada e suas especificidades com a finalidade de exemplificar as tensões e conflitos que envolvem o pertencimento a um grupo social. As fontes analisadas encontram-se no acervo da instituição, ainda em funcionamento nos dias de hoje.

  13. Redes bayesianas para inferência de redes regulatórias de genes

    OpenAIRE

    2005-01-01

    Nos últimos anos, um grande volume de dados de várias espécies vem sendo obtido através de novas técnicas criadas e aperfeiçoadas pela biologia. Entre elas, tecnologias para medir as diferenças das expressões dos genes, através de concentrações de mRNA (microarray), estão se tornando extremamente populares e seus custos estão diminuindo. A inferência de redes regulatórias de genes a partir de dados de expressão gênica para estudar o metabolismo dos organismos é um processo impo...

  14. Título da Página electrónica: Social Movements International Secretariat

    Directory of Open Access Journals (Sweden)

    Vanda Pacheco

    2012-10-01

    Full Text Available Esta página, disponível em inglês e castelhano, foi criada por um conjunto de activistas de movimentos sociais, que em comum dizer ter a luta contra a “globalização neo-liberal, a guerra, o racismo, o sistema de castas, o fanatismo religioso, a pobreza , o patriarcado e todas as formas de discriminação e exclusão económica, étnica, social, política, cultural, sexual e de género”. Destacam-se os documentos produzidos no âmbito dos cinco Fóruns Sociais Mundiais (FSM já realizados, tais como as...

  15. Sujeito da enunciação: singularidade que advém da sintaxe da enunciação Enunciative subject: singularity derived from the enunciative syntax

    Directory of Open Access Journals (Sweden)

    Valdir do Nascimento Flores

    2013-01-01

    Full Text Available Este texto, a partir da leitura da teoria enunciativa criada por Émile Benveniste, desenvolve a noção de sujeito da enunciação ligando-a, metodologicamente, à noção de sintaxe de enunciação como forma de propor uma possibilidade de análise de fatos de língua. O texto ilustra a proposta metodológica em um dado de linguagem infantil.This text, based on the reading of the enunciative theory created by Emile Benveniste, develops the notion of enunciative subject, methodologically linking it to the notion of enunciative syntax in order to propose a possibility for analyzing facts of language. The text illustrates this methodological proposal using data from child language.

  16. Joseph Roth, tradutor do Império perdido

    Directory of Open Access Journals (Sweden)

    Luis S. Krausz

    2015-06-01

    Full Text Available Este artigo discute um folhetim jornalístico do escritor austríaco Joseph Roth que trata de uma miniatura do Templo de Salomão, encontrada por ele num café da Hirtenstrasse berlinense, no coração do que foi, na década de 1920, o bairro habitado pelos judeus do Leste da Europa na capital alemã. A nostalgia, sob cujo signo foi criada tal miniatura, torna-se uma metáfora da nostalgia pelo império Austro-Húngaro, chave para a compreensão da obra romanesca deste escritor. Ao mesmo tempo, esta nostalgia rothiana é contextualizada no âmbito das crenças místicas e messiânicas do judaísmo tradicional.

  17. A Revolução Russa e a construção da III Internacional

    Directory of Open Access Journals (Sweden)

    Carlos Prado

    2017-02-01

    Full Text Available O presente texto analisa a influência que a revolução russa exerceu sobre a construção da III Internacional (Comintern. Num primeiro momento investiga-se a guerra e a falência da II Internacional. Posteriormente aborda-se o processo e os debates que antecederam a construção da nova organização. Por fim, analisam-se os documentos produzidos pelo primeiro congresso da III Internacional, evidenciando como a experiência russa, influenciou o programa da Comintern. Ao apontar a necessidade da tomada do poder do Estado e da construção da ditadura do proletariado pelo regime dos sovietes, a recém-criada Internacional Comunista estava abraçando o modelo russo de revolução.

  18. Título da página electrónica: Escola de Cultura de Paz

    Directory of Open Access Journals (Sweden)

    Mónica Rafael Simões

    2012-10-01

    Full Text Available A Escola de Cultura de Paz foi criada em 1999, dando seguimento à Cátedra UNESCO sobre Paz e Direitos Humanos da Universidade Autónoma de Barcelona, com o objectivo de trabalhar para uma cultura de paz, promovendo a investigação e formação em áreas relacionadas com prevenção e análise de conflitos, processos de paz, desarmamento, direitos humanos e educação para a paz. Esta página é coordenada por Vicenç Fisas e produzida pela sua equipa de jovens investigadores da Universidade Autónoma de B...

  19. Título da página electrónica: Escola de Cultura de Paz

    OpenAIRE

    Mónica Rafael Simões

    2005-01-01

    A Escola de Cultura de Paz foi criada em 1999, dando seguimento à Cátedra UNESCO sobre Paz e Direitos Humanos da Universidade Autónoma de Barcelona, com o objectivo de trabalhar para uma cultura de paz, promovendo a investigação e formação em áreas relacionadas com prevenção e análise de conflitos, processos de paz, desarmamento, direitos humanos e educação para a paz. Esta página é coordenada por Vicenç Fisas e produzida pela sua equipa de jovens investigadores da Universidade Autónoma de B...

  20. Título da página electrónica: Social Movements International Network/Red Internacional de los Movimientos Sociales

    Directory of Open Access Journals (Sweden)

    José Manuel Mendes

    2012-10-01

    Full Text Available No âmbito do Fórum Social Mundial, foi criada a Rede Internacional de Movimentos Sociais, com sede em São Paulo. Esta é a página dessa Rede, que congrega um vasto conjunto de movimentos sociais de diferentes países que têm como objectivo principal a luta contra a globalização neoliberal e todas as formas de opressão e exploração a nível mundial. A página apresenta-se em edição bilingue, em inglês e espanhol, e abre com as conclusões dos encontros internacionais realizados e com a agenda da Re...

  1. Primitivo, naïf, ingênuo: um estudo da recepção e notas para uma interpretação da pintura de Heitor dos Prazeres

    OpenAIRE

    Patricia Miranda D\\'Avila

    2009-01-01

    A proposta da pesquisa consistiu em analisar quatro assuntos pertinentes à compreensão da pintura de Heitor dos Prazeres. Em primeiro lugar, conhecer historicamente o ambiente no qual as obras foram criadas e a relação do artista com o universo do samba carioca, em segundo, recolher análises críticas que atestassem a recepção dessa produção, em terceiro, analisar resquícios ou elementos que comprovem a proximidade da invenção do artista em questão com referências ao imaginário de herança afri...

  2. Título do sítio electrónico: Fédération Européenne des Associations Nacionales Travaillant avec les Sans-Abri e European Observatory on Homelessness

    Directory of Open Access Journals (Sweden)

    João Aldeia

    2012-10-01

    Full Text Available Optou-se por apresentar conjuntamente as duas páginas pela sua estreita articulação temática e institucional. Criada em 1989, a FEANTSA – Fédération Européenne des Associations Nacionales Travaillant avec les Sans-Abri – é uma organização não-governamental (ONG dedicada a eliminar a pobreza e exclusão social de indivíduos sem-abrigo ou em risco de conhecer esta situação, que congrega mais de 100 ONGs locais e nacionais de vários Estados-membros da União Europeia, sendo financiada pela Comiss...

  3. O processo de reabilitação de edifícios abandonados em Vitória-ES: acertos e entraves

    OpenAIRE

    Vívian Gazzoli Araújo

    2012-01-01

    A partir da década de 70 os centros das grandes cidades brasileiras passaram por um esvaziamento ocasionado pelo deslocamento da população em direção às novas centralidades. Isso provocou uma redução da densidade demográfica da região central e o surgimento de edificações vazias. Com a criação do Ministério das Cidades foram criadas algumas políticas públicas que incentivam a reabilitação dessas regiões abandonas e a revitalização de edifícios de valor histórico. Em Vitória, para reduzir o dé...

  4. Responsividade do serviço de enfermagem na visão do cliente Responsividad del servicio de enfermería en la visión del paciente Nursing care responsiveness from the client's view

    OpenAIRE

    Ana Vanessa Deffaccio Rodrigues; Dagmar Willamowius Vituri; Maria do Carmo Lourenço Haddad; Marli Terezinha Oliveira Vannuchi; William Tiago Oliveira

    2012-01-01

    A expectativa criada pelo indivíduo na hospitalização e a percepção que ele tem do cuidado que recebe quando hospitalizado são aspectos que definem seu grau de satisfação. Assim, surgiu o conceito responsividade no campo da avaliação em saúde. O objetivo deste estudo é mensurar a responsividade do serviço de enfermagem de uma unidade médico-cirúrgica de um hospital universitário público, na visão do cliente, por meio de um instrumento que aborda duas categorias: Expectativas e Percepções. Tra...

  5. O Ecoturismo em Santo Antônio do Itambé (MG) como indutor do envolvimento comunitário no entorno de Unidades de Conservação

    OpenAIRE

    2013-01-01

    Este artigo visa refletir sobre a forma com que o ecoturismo induz o envolvimento das comunidades localizadas no entorno de Unidades de Conservação, considerando que elas são criadas com intuito, também, de uso público, no qual o turismo se enquadra e é visto pelo governo como importante ação para preservação das Unidades de Conservação e valorização das culturas das comunidades de seu entorno. É proposto o estudo de caso da pequena cidade de Santo Antônio do Itambé para observar a influência...

  6. PERSPECTIVAS PARA CONSTRUÇÃO PARTICIPATIVA DO ECOTURISMO DE BASE COMUNITÁRIA NA RESEX CASSURUBÁ

    OpenAIRE

    2011-01-01

    Nos municípios de Caravelas e Nova Viçosa, extremo sul da Bahia, existe o complexo estuarino Cassurubá que, por sua importância social, cultural e ambiental, está inserido na área da recém criada Reserva Extrativista Cassurubá. Nesta unidade de conservação de uso sustentável está sendo proposto um projeto de ecoturismo de base comunitária que envolve 15 famílias ribeirinhas e 13 instituições atuantes na localidade. A finalidade do projeto é iniciar a implantação deste tipo de turismo proporci...

  7. As Guerras Justas e a escravidão indígena em Minas Gerais nos séculos XVIII e XIX The "Fair Wars" and the indigenous slavery in Minas Gerais in the XVIII and XIX centuries

    Directory of Open Access Journals (Sweden)

    Marcia Amantino

    2006-06-01

    Full Text Available Este artigo busca analisar as diferentes imagens que foram criadas sobre os índios em Minas Gerais demonstrando que, na maioria das vezes, determinados grupamentos indígenas foram associados às feras. Assim, todas as medidas repressivas contra suas aldeias eram justificadas, inclusive, as Guerras Justas. O artigo analisa também alguns documentos sobre a escravidão indígena na região até pelo menos, o século XIX.This article searches to analyses the different images form created about the Indians in Minas Gerais, demonstrating that, in most times, determined aboriginal groupings were seen like wild animals. Thus, all the repressive measures taken against its villages had been justified, also, the "Fair Wars"... The article also analyses some documents about aboriginal slavery in that region until at least, century XIX.

  8. A universalidade em grande sertão: veredas e a noção de gramatica universaL

    Directory of Open Access Journals (Sweden)

    Lorenzo VITRAL

    2009-07-01

    Full Text Available Este artigo propõe uma reflexão sobre a sintaxe criada por Guimarães Rosa na sua obra-prima Grande sertão: veredas, Admite-se normalmente que sua linguagem utiliza volteios e estruturas provenientes de línguas estrangeiras, de certos dialetos falados do português do Brasil e de estágios históricos do português, Propomos que esses recursos são tão-somente elementos que incitam mecanismos da Gramática Universal, isto é, a entidade teórica proposta na teoria da Gramática Gerativa.

  9. A universalidade em grande sertão: veredas e a noção de gramatica universaL

    Directory of Open Access Journals (Sweden)

    Lorenzo VITRAL

    2013-11-01

    Full Text Available Este artigo propõe uma reflexão sobre a sintaxe criada por Guimarães Rosa na sua obra-prima Grande sertão: veredas, Admite-se normalmente que sua linguagem utiliza volteios e estruturas provenientes de línguas estrangeiras, de certos dialetos falados do português do Brasil e de estágios históricos do português, Propomos que esses recursos são tão-somente elementos que incitam mecanismos da Gramática Universal, isto é, a entidade teórica proposta na teoria da Gramática Gerativa.

  10. Título da página electrónica: 1968 in Europe

    Directory of Open Access Journals (Sweden)

    Miguel Cardina

    2012-10-01

    Full Text Available Se os protestos estudantis ocorridos em Paris ou Praga durante o “longo ano” de 1968 são alvos de frequente evocação, bem menor é a visibilidade dos processos contestatários surgidos em outras paragens do território europeu, situadas em qualquer uma das metades criadas pela linha divisória do antigo muro de Berlim. Este site tem desde logo o mérito de apresentar cerca de uma vintena de cronologias, alinhadas por critérios geográficos ou temáticos, cuja leitura permite perceber o carácter tran...

  11. Avaliação histometrica da regeneração periodontal obtida com o uso de membranas reabsorviveis de acido polilactico em defeitos periodontais em cães

    OpenAIRE

    Enilson Antonio Sallum

    1996-01-01

    Resumo: O objetivo deste estudo foi avaliar o uso das membranas reabsorvíveis (GUIDOR `MARCA REGISTRADA´) no tratamento de defeitos periodontais em cães, utilizando análise histométrica, comparando com o uso da terapia convencional. Deiscências ósseas vestibulares (3x5mm) foram criadas nas raízes distais dos terceiros pré-molares mandibulares (P3) em 5 cães e uma tira matriz metálica tipo Toffelmire foi fixada na área. Três meses após a fabricação do defeito, as bandas metálicas foram removid...

  12. Société Musicale Indépendante – SMI, sua fundação, sua bandeira e o testemunho da imprensa especializada em torno de sua primeira temporada artística

    Directory of Open Access Journals (Sweden)

    Danieli Verônica Longo Benedetti

    2015-06-01

    Full Text Available O presente artigo, segmento de pesquisa de Pós-Doutorado sediada no Departamento de Música da ECA/USP e amparada pela FAPESP, pretende traçar o histórico da Société Musicale Indépendante – SMI, sociedade musical francesa criada em 1909 por um grupo de compositores entre os quais Maurice Ravel, Charles Koechlin e Florent Schmitt, cujo principal objetivo foi promover a música contemporânea sem distinção de escola e nacionalidade. Fundamentado em material coletado no acervo privado de Charles Koechlin e da Bibliothèque nationale de France-BnF o texto pretende ainda uma reflexão sobre a primeira temporada artística da SMI por meio do testemunho da crítica especializada publicado por importantes órgãos de imprensa da época.

  13. Importância das imagens na metodologia de pesquisa em psicologia social

    OpenAIRE

    Medina Filho,Antonio Luiz de

    2013-01-01

    Discussão dos limites derivados da utilização praticamente exclusiva da linguagem verbal na metodologia de pesquisa em ciências humanas e sociais e proposição de uma reflexão sobre a incorporação sistemática de outras dimensões linguísticas, em especial a utilização de imagens criadas pelos sujeitos da pesquisa. A expressão imagética articulada com a expressão verbal fornece uma riqueza de informação que possibilita a ampliação das possibilidades de eficácia das pesquisas podendo levar a resu...

  14. Risco de quedas nas UCCI do distrito de Viseu : estudo comparativo escala de Morse e escala de Dowton

    OpenAIRE

    Almeida, Catarina Inês Soares; Duarte, João Carvalho; Chaves, Cláudia Margarida Correia Balula, co-orient.

    2012-01-01

    Enquadramento: O envelhecimento e as alterações na estrutura social e familiar determinam o aparecimento de novas necessidades, cuja satisfação reclama mudanças tanto a nível de saúde como a nível social, que devem ser capazes de desenvolver respostas que possam contribuir para a manutenção e restauração da dignidade e da qualidade de vida. Neste contexto através da Resolução do Conselho de Ministros nº 84/2005 foi criada na dependência do Ministério da Saúde, a Comissão para o Desenvolviment...

  15. Differences in wing melanization and pigmentation pattern in Drosophila buzzatii (Diptera: Drosophilidae under chemical stress

    Directory of Open Access Journals (Sweden)

    Nicolás MONGIARDINO KOCH

    2012-01-01

    Full Text Available Recientemente, hemos avanzado en nuestra comprensión de las bases genéticas y los mecanismos subyacentes al proceso de melanización durante el desarrollo, lo que permite el estudio de los procesos evolutivos que gobiernan la variabilidad de pigmentación presente en la naturaleza. Sin embargo, los factores ambientales que contribuyen a dicha variabilidad han recibido poca atención, aún cuando pueden conllevar profundas consecuencias a nivel ecológico. En este trabajo, presentamos un método para analizar los patrones de melanización alar de drosofílidos, de forma cualitativa y cuantitativa. Ponemos a prueba esta metodología comparando las alas de Drosophila buzzatii Patterson & Wheeler, criadas en medio control y con el agregado de alcaloides. Los alcaloides fueron extraídos del huésped secundario de estas moscas, Echinopsis terscheckii (Parm. ex Pfeiff. Friedrich & Rowley, los cuales, se sabe, presentan efectos negativos en el desarrollo de D. buzzatii. Las moscas criadas en un medio enriquecido con alcaloides mostraron una mayor melanización alar, junto con diferencias en la distribución espacial del pigmento. Planteamos modificaciones a nivel metabólico y de expresión génica para explicar los cambios que las condiciones de estrés inducen sobre el carácter. Proponemos la utilización de este procedimiento para el estudio de la dependencia ambiental de la melanización.

  16. Exercer a Postura Crítica: Desafios no Estágio em Psicologia Escolar

    Directory of Open Access Journals (Sweden)

    Adriana Marcondes Machado

    Full Text Available Os estudantes de graduação do curso de Psicologia do Instituto de Psicologia da Universidade de São Paulo, ao cursarem as disciplinas relacionadas à área da Psicologia Escolar, realizam estágios em instituições educativas. Imbuídos de uma postura crítica, os estagiários entram em contato com demandas de tratamento, prevenção e adaptação e compreendem a produção histórica destas. Todavia, em muitos momentos, os estagiários julgam essas demandas como se fossem equivocadas e, dessa forma, não conseguem perceber a multiplicidade de elementos que as constituem e as formas para agir em sua produção. O objetivo deste artigo é apresentar um percurso de trabalho enfatizando as estratégias criadas para dar conta de um dos desafios que se apresentam no grupo de supervisão: intervir nesse momento no qual os estagiários consideram o funcionamento da escola como algo exterior a eles. As estratégias criadas indicam que a desconstrução necessária na formação dos estudantes de Psicologia para que aprendam a intervir nos processos institucionais vividos durante os estágios requer, também, a desconstrução de uma forma de pensar que cria um suposto sujeito fora do diagrama de forças em que o processo do estágio faz parte.

  17. Efeito da Densidade de Criação e do Sexo Sobre o Empenamento, Incidência de Lesões na Carcaça e Qualidade da Carne de Peito de Frangos de Corte

    Directory of Open Access Journals (Sweden)

    Garcia RG

    2002-01-01

    Full Text Available O experimento foi conduzido nas instalações experimentais da Faculdade de Medicina Veterinária e Zootecnia - UNESP, Campus de Botucatu, SP, Brasil, com o objetivo de avaliar o efeito da densidade de criação e do sexo sobre o empenamento, incidência de lesões na carcaça e a qualidade de carne de peito de frangos de corte. Foram utilizados 1950 pintos de corte sexados, da linhagem Ross, distribuídos em um delineamento inteiramente casualizado, com esquema fatorial com 3 densidades (10, 13 e 16 aves/m² e dois sexos com 5 repetições, sendo que uma foi destinada exclusivamente para reserva, criados até os 42 dias de idade. Aos 28, 35 e 42 dias foram amostradas 3 aves por repetição para a determinação do empenamento através da porcentagem de penas e 10 aves para a determinação do escore de empenamento. Também aos 42 dias de idade todas as aves foram identificadas na pata com anilhas numeradas e submetidas à avaliação da incidência de lesões na pele. Foram escolhidas ao acaso 5 aves por repetição para a determinação da qualidade da carne de peito. Pode-se concluir que o aumento na densidade de criação promoveu uma diminuição na velocidade de empenamento e, conseqüentemente, uma maior incidência de lesões na carcaça. O comprimento, a largura e a espessura do peito foram menores para as aves criadas na maior densidade, e a perda de peso por cozimento foi maior para as aves criadas na maior densidade.

  18. A Indústria de Petróleo no Brasil: avaliação histórica da concepção da empresa Petrobrás

    Directory of Open Access Journals (Sweden)

    Paula Meyer Soares

    2012-06-01

    Full Text Available A indústria petrolifera no Brasil remonta os anos 50. A crise mundial que eclodiu nos anos 30 impactou fortemente na trajetoria de crescimento da economia brasileira e de vários outros países. Inicia-se entao um lento e gradual processo de transição do modelo de crescimento economico assentado no setor externo para um modelo de substituição de importações cujo objetivo principal era fundar um parque industrial forte e competitivo capaz de gerar renda e empregos a solidez desse modelo dependia da consolidação de uma industria de base e pesada (infraestrutura sólidas. Nesse sentido foi criada a empresa Petrobrás, durante o Governo Vargas e desde então o Brasil tornou-se pioneiro na exploração e produção de petróleo no mercado mundial. O referido estudo fará uma retrsopectiva historica da industria petrolifera no Brasil considerando as suas particularidades e evolução do marco regulatório. A ideia central é analisar o contexto em que foi criada a Petrobrás e principais resultados alcancados nesse segmento nos dias de hoje. A metodologia utilizada baseou-se em referencial teórico que aborda o assunto e dados estatisticos que ilustrem essas mudanças regulatórias e economicas nesses anos desde a sua criação.

  19. Nomenclatural Studies Toward a World List of Diptera Genus-Group Names. Part IV: Charles Henry Tyler Townsend.

    Science.gov (United States)

    Evenhuis, Neal L; Pont, Adrian C; Whitmore, Daniel

    2015-06-25

    ]; Azygobothria Townsend, 1911 [Tachinidae]; Brachymasicera Townsend, 1911 [Tachinidae]; Calocarcelia Townsend, 1927 [Tachinidae]; Cnephalodes Townsend, 1911 [Tachinidae]; Cyacyrtoneura Townsend, 1931 [Muscidae]; Cyrtoneuropsis Townsend, 1931 [Muscidae]; Cyrtosoma Brauer & Bergenstamm, 1893 [Tachinidae]; Epiphyllophila Townsend, 1927 [Tachinidae]; Eucalodexia Townsend, 1892 [Tachinidae]; Eumesembrina Townsend, 1908 [Muscidae]; Eumyobia Townsend, 1911 [Tachinidae]; Eusisyropa Townsend, 1908 [Tachinidae]; Gabanimyia Townsend, 1914 [Tachinidae]; Galactomyia Townsend, 1908 [Tachinidae]; Girschneria Townsend, 1919 [Tachinidae]; Gymnochaetopsis Townsend, 1914 [Tachinidae]; Himantostomopsis Townsend, 1921 [Tachinidae]; Incamyia Townsend, 1912 [Tachinidae]; Lithoexorista Townsend, 1921 [Tachinidae]; Muscopteryx Townsend, 1892 [Tachinidae]; Myocuphocera Townsend, 1931 [Tachinidae]; Myxexoristops Townsend, 1911 [Tachinidae]; Neojurinia Townsend, 1914 [Tachinidae]; Newsteadina Townsend, 1921 [Glossinidae]; Ommasicera Townsend, 1911 [Tachinidae]; Ophirion Townsend, 1911 [Tachinidae]; Ophiriodexia Townsend, 1911 [Tachinidae]; Ophiriosturmia Townsend, 1911 [Tachinidae]; Opsozelia Townsend, 1919 [Tachinidae]; Paleotachina Townsend, 1921 [Tachinidae]; Palexorista Townsend, 1921 [Tachinidae]; Phasiatacta Townsend, 1911 [Tachinidae]; Philippolophosia Townsend, 1927 [Tachinidae]; Phrissopolia Townsend, 1908 [Tachinidae]; Pseudokea Townsend, 1927 [Tachinidae]; Pygocalcager Townsend, 1935 [Tachinidae]; Trichobius Townsend, 1891 [Hippoboscidae]; Villeneuvia Townsend, 1921 [Tachinidae]; Zonoepalpus Townsend, 1927 [Tachinidae]; Zygosturmia Townsend, 1911 [Tachinidae].        The following names previously treated as available are shown to be unavailable.-Genera: Denatella Townsend, 1931, n. stat. [Calliphoridae]; Epseudocyptera Townsend, 1927, n. stat. [Tachinidae]; Eustomatodexia Townsend, 1892, n. stat. [Tachinidae].-Species: Epseudocyptera epalpata Townsend, 1927, n. stat. [Tachinidae

  20. The Complete Mitochondrial Genome of Delia antiqua and Its Implications in Dipteran Phylogenetics.

    Directory of Open Access Journals (Sweden)

    Nai-Xin Zhang

    Full Text Available Delia antiqua is a major underground agricultural pest widely distributed in Asia, Europe and North America. In this study, we sequenced and annotated the complete mitochondrial genome of this species, which is the first report of complete mitochondrial genome in the family Anthomyiidae. This genome is a double-stranded circular molecule with a length of 16,141 bp and an A+T content of 78.5%. It contains 37 genes (13 protein-coding genes, 22 tRNAs and 2 rRNAs and a non-coding A+T rich region or control region. The mitochondrial genome of Delia antiqua presents a clear bias in nucleotide composition with a positive AT-skew and a negative GC-skew. All of the 13 protein-coding genes use ATN as an initiation codon except for the COI gene that starts with ATCA. Most protein-coding genes have complete termination codons but COII and ND5 that have the incomplete termination codon T. This bias is reflected in both codon usage and amino acid composition. The protein-coding genes in the D. antiqua mitochondrial genome prefer to use the codon UUA (Leu. All of the tRNAs have the typical clover-leaf structure, except for tRNASer(AGN that does not contain the dihydrouridine (DHU arm like in many other insects. There are 7 mismatches with U-U in the tRNAs. The location and structure of the two rRNAs are conservative and stable when compared with other insects. The control region between 12S rRNA and tRNAIle has the highest A+T content of 93.7% in the D. antiqua mitochondrial genome. The control region includes three kinds of special regions, two highly conserved poly-T stretches, a (TAn stretch and several G(AnT structures considered important elements related to replication and transcription. The nucleotide sequences of 13 protein-coding genes are used to construct the phylogenetics of 26 representative Dipteran species. Both maximum likelihood and Bayesian inference analyses suggest a closer relationship of D. antiqua in Anthomyiidae with Calliphoridae

  1. Key to the adults of the most common forensic species of Diptera in South America Chave de identificação para as espécies comuns de Diptera da América do Sul de interesse forense

    Directory of Open Access Journals (Sweden)

    Claudio José Barros de Carvalho

    2008-09-01

    Full Text Available Flies (Diptera, blow flies, house flies, flesh flies, horse flies, cattle flies, deer flies, midges and mosquitoes are among the four megadiverse insect orders. Several species quickly colonize human cadavers and are potentially useful in forensic studies. One of the major problems with carrion fly identification is the lack of taxonomists or available keys that can identify even the most common species sometimes resulting in erroneous identification. Here we present a key to the adults of 12 families of Diptera whose species are found on carrion, including human corpses. Also, a summary for the most common families of forensic importance in South America, along with a key to the most common species of Calliphoridae, Muscidae, and Fanniidae and to the genera of Sarcophagidae are provided. Drawings of the most important characters for identification are also included.Diptera (califorídeos, sarcofagídeos, motucas, moscas comuns e mosquitos é a uma das quatro ordens megadiversas de insetos. Diversas espécies desta ordem podem rapidamente colonizar cadáveres humanos e são de utilidade potencial para estudos de entomologia forense. Um dos maiores problemas com moscas que visitam matéria orgânica animal em decomposição é a falta de taxonomistas ou chaves de identificação disponíveis que possam identificar as espécies mais comuns ou mesmo, algumas vezes podendo resultar em identificações errôneas. Neste artigo é apresentada uma chave para adultos de 12 famílias de Diptera com espécies encontradas em matéria orgânica animal em decomposição, incluindo cadáveres humanos. Também é incluído um sumário das mais importantes famílias com espécies de interesse forense na América do Sul e chave de identificação das espécies mais comuns de Calliphoridae, Muscidae e Fanniidae e dos gêneros de Sarcophagidae. Esquemas dos mais importantes caracteres utilizados para identificação dessas espécies são também incluídos.

  2. Determinación del intervalo postmortem mediante el estudio de la sucesión de insectos en dos cadáveres hallados en el interior de una finca rústica en Madrid Assessment of post-mortem interval through the study of the insect succession in two corpses found indoors in a country house in Madrid

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    A.M. García-Rojo

    2009-04-01

    Full Text Available En este artículo se presenta un caso estudiado en el Laboratorio de Entomología Forense de la Sección de Antropología de la Comisaría General de Policía Científica (Cuerpo Nacional de Policía. En él, se analizaron muestras entomológicas recogidas en dos cadáveres durante la práctica de la Inspección Ocular Técnico Policial, llevada a cabo en la localidad de Fuenlabrada, sita en la zona Sur de Madrid (España, Región Paleártica. Los dos cadáveres, en avanzado estado de descomposición, fueron encontrados en el interior de una finca rústica en invierno del año 2001. Las evidencias de interés forense recogidas consistieron en dípteros de las Familias Calliphoridae, Muscidae y Phoridae y Coleoptera de las Familias Cleridae, Dermestidae e Histeridae. Además, arácnidos que permitieron afirmar la compatibilidad de los especímenes con el entorno. La sucesión de los insectos recogidos permitió estimar el intervalo post-mortem en un año.In this paper, it is reported a case which was studied in the Forensic Entomology Laboratory belonging to the Anthropology Section from the General Department of the Scientific Police (Spanish National Police. We analysed entomological evidences collected during death scene investigation performed in Fuenlabrada, south of Madrid administrative region (Spain, Paleartic Region. Both corpses, in advanced decomposition stage, were found indoors in a country house in winter 2001. Samples collected consisted of Diptera identified as Calliphoridae, Muscidae and Phoridae Families; Coleoptera corresponding to Cleridae, Dermestidae and Histeridae ones. Furthermore, spiders which allowed us to confirm the compatibility with the place where carcasses were found. The study of the insect succession allowed the estimation of post-mortem interval in one year.

  3. Using bacterial and necrophagous insect dynamics for post-mortem interval estimation during cold season: Novel case study in Romania.

    Science.gov (United States)

    Iancu, Lavinia; Carter, David O; Junkins, Emily N; Purcarea, Cristina

    2015-09-01

    Considering the biogeographical characteristics of forensic entomology, and the recent development of forensic microbiology as a complementary approach for post-mortem interval estimation, the current study focused on characterizing the succession of necrophagous insect species and bacterial communities inhabiting the rectum and mouth cavities of swine (Sus scrofa domesticus) carcasses during a cold season outdoor experiment in an urban natural environment of Bucharest, Romania. We monitored the decomposition process of three swine carcasses during a 7 month period (November 2012-May 2013) corresponding to winter and spring periods of a temperate climate region. The carcasses, protected by wire cages, were placed on the ground in a park type environment, while the meteorological parameters were constantly recorded. The succession of necrophagous Diptera and Coleoptera taxa was monitored weekly, both the adult and larval stages, and the species were identified both by morphological and genetic characterization. The structure of bacterial communities from swine rectum and mouth tissues was characterized during the same time intervals by denaturing gradient gel electrophoresis (DGGE) and sequencing of 16S rRNA gene fragments. We observed a shift in the structure of both insect and bacterial communities, primarily due to seasonal effects and the depletion of the carcass. A total of 14 Diptera and 6 Coleoptera species were recorded on the swine carcasses, from which Calliphora vomitoria and C. vicina (Diptera: Calliphoridae), Necrobia violacea (Coleoptera: Cleridae) and Thanatophilus rugosus (Coleoptera: Silphidae) were observed as predominant species. The first colonizing wave, primarily Calliphoridae, was observed after 15 weeks when the temperature increased to 13°C. This was followed by Muscidae, Fanniidae, Anthomyiidae, Sepsidae and Piophilidae. Families belonging to Coleoptera Order were observed at week 18 when temperatures raised above 18°C, starting with

  4. The Complete Mitochondrial Genome of Delia antiqua and Its Implications in Dipteran Phylogenetics.

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    Zhang, Nai-Xin; Yu, Guo; Li, Ting-Jing; He, Qi-Yi; Zhou, Yong; Si, Feng-Ling; Ren, Shuang; Chen, Bin

    2015-01-01

    Delia antiqua is a major underground agricultural pest widely distributed in Asia, Europe and North America. In this study, we sequenced and annotated the complete mitochondrial genome of this species, which is the first report of complete mitochondrial genome in the family Anthomyiidae. This genome is a double-stranded circular molecule with a length of 16,141 bp and an A+T content of 78.5%. It contains 37 genes (13 protein-coding genes, 22 tRNAs and 2 rRNAs) and a non-coding A+T rich region or control region. The mitochondrial genome of Delia antiqua presents a clear bias in nucleotide composition with a positive AT-skew and a negative GC-skew. All of the 13 protein-coding genes use ATN as an initiation codon except for the COI gene that starts with ATCA. Most protein-coding genes have complete termination codons but COII and ND5 that have the incomplete termination codon T. This bias is reflected in both codon usage and amino acid composition. The protein-coding genes in the D. antiqua mitochondrial genome prefer to use the codon UUA (Leu). All of the tRNAs have the typical clover-leaf structure, except for tRNASer(AGN) that does not contain the dihydrouridine (DHU) arm like in many other insects. There are 7 mismatches with U-U in the tRNAs. The location and structure of the two rRNAs are conservative and stable when compared with other insects. The control region between 12S rRNA and tRNAIle has the highest A+T content of 93.7% in the D. antiqua mitochondrial genome. The control region includes three kinds of special regions, two highly conserved poly-T stretches, a (TA)n stretch and several G(A)nT structures considered important elements related to replication and transcription. The nucleotide sequences of 13 protein-coding genes are used to construct the phylogenetics of 26 representative Dipteran species. Both maximum likelihood and Bayesian inference analyses suggest a closer relationship of D. antiqua in Anthomyiidae with Calliphoridae, Calliphoridae is a

  5. Sampling necrophagous and predatory insects using different lures in a Mexican pine forest Muestreo de insectos necrófagos y depredadores utilizando diferentes cebos en un bosque de pino en México

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    Gerardo Sánchez-Rojas

    2011-09-01

    Full Text Available Sampling with a single bait as a lure is a commonly used practice in studies of necrophilous insect communities. Here, we determined if there were differences in necrophilous insect species richness and abundance when attracted to 4 types of carrion: squid, bird, lizard, and mouse. A comparison between necrophagous and predatory insects was also made to detect whether they differ in their abundance, depending on the bait used. Thirteen species of Silphidae, Staphylinidae (Coleoptera, Calliphoridae, and Sarcophagidae (Diptera were collected in the baited traps; no single bait was able to attract all species. The abundances of 5 species varied significantly depending on the type of bait used. Predatory insects were less abundant than necrophagous insects in lizard and mouse baits. These results show that an evaluation of the structure of a necrophilous insect community can depend on the bait used for sampling.La utilización de un solo tipo de cebo es una práctica común en los estudios de comunidades de insectos necrófilos. En este trabajo determinamos si existen diferencias en la riqueza y abundancia de especies de insectos necrófilos atraídos a 4 tipos de carroña: calamar, ave, lagartija y ratón. También se determinó si la abundancia de insectos necrófagos y depredadores difiere dependiendo del tipo de cebo que se utilice. Se recolectaron 13 especies de Silphidae, Staphylinidae (Coleoptera, Calliphoridae y Sarcophagidae (Diptera; ningún tipo de cebo logró atraer a todas las especies. Se encontró que la abundancia de 5 de las especies difirió significativamente dependiendo del cebo utilizado. La abundancia de los insectos depredadores fue menor que la de los carroñeros en los cebos de lagartija y ratón. Estos resultados muestran que la evaluación de la estructura de una comunidad de insectos necrófilos puede depender del tipo de cebo que se utilice.

  6. 三种蝇类昆虫(双翅目)足爪垫结构的超微形态研究%ULTRASTRUCTURAL MORPHOLOGY OF THE PULVILLI IN MUSCA DOMESTICA, BERCAEA CRUENTATA AND CHRYSOMYA PINGUIS (DIPTERA)

    Institute of Scientific and Technical Information of China (English)

    张学书; 梁爱萍

    2012-01-01

    利用扫描电子显微镜对双翅目3种蝇类昆虫即家蝇Musca domestica L.(蝇科)、红尾粪麻蝇Bercaea cruentata(Meigen)(麻蝇科)和肥躯金蝇Chrysomya pinguis (Walker)(丽蝇科)足的爪垫超微形态结构进行了研究,研究发现每种蝇类的前、中、后足爪垫的面积存在一定的差别,中足和后足爪垫面积较前足大;爪垫腹面均密被刚毛,每根刚毛由刚毛杆(setal shaft)和末端平板(terminal plate)组成,刚毛主要有铲状、勺状和柳叶状3种类型;后足爪垫上刚毛的密度和刚毛末端面积一般小于前、中足;位于爪垫边缘处的刚毛较长.研究还发现爪垫上的刚毛均为中空结构,且丽蝇科昆虫的刚毛末端具开口.%The ultrastructure of the pulvilli of three fly species, viz. Musca domestica L. (Muscidae), Bercaea cruentata ( Meigen ) ( Sarcophagidae ) and Chrysomya pinguis (Walker) (Calliphoridae) was studied with the scanning electron microscopy. In each species, the size of the ventral surface of pulvilli on the fore, middle and hind legs is different. The ventral area of pulvilli on the middle and hind legs is larger than that of the fore legs. The ventral surface of pulvilli is fully covered with numerous tenent setae. The setae consists of the basal elongate setal shaft and an distal tip. Three kinds of tenent setae, viz. spatula-like, spoon-like and willow leaf-like tenent setae were found. The setal shaft on marginal area of the pulvillus is longer than that in other areas. Both the size of the tip area and the density of tenent setae of pulvilli on hind legs are smaller than those on die fore and middle legs. The setal shaft is hollow centrally. A ventral opening on the setal tip was observed in Chrysomya pinguis (Calliphoridae).

  7. New records of Muscidae (Diptera in Campo Grande, MS, Brazil Novos registros de dípteros muscóides em Campo Grande, MS, Brasil

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    Hera Luana Luiz

    2012-12-01

    Full Text Available Synanthropic flies outstanding beside other flies due their relative abundance close to domestic animals and human population, to which they are able to cause myiasis or transmit pathogenic agents. As they're necrophagous they act as corpse decomposers and are useful in the forensic entomology in the post mortem interval determination. This study aimed to know flies diversity and abundance in Campo Grande, State of Mato Grosso do Sul. Captures were made weekly, utilizing three traps baited with decaying fish meat, from June of 2008 to May of 2009 in a remainder ciliary forest of the Embrapa's Cattle Beef Experimental Farm. The dipterans families that were considered and respectively number of collected specimens were: Calliphoridae (105,334; Muscidae (27,999; Sarcophagidae (21,083; Fanniidae (17,759 and Mesembrinellidae (305, totalizing 172,480 dipterous. To the local known species some Muscidae were increased as follows: Neomuscina atincticosta, Pseudoptilolepis elbida, Polietina orbitalis, Polietina flavithorax, Scutellomusca scutellaris, Graphomya analis and Morellia couriae.As moscas sinantrópicas se destacam pelo fato de serem relativamente abundantes junto à população humana e animais domésticos, podendo causar a estes miíases ou transmitir agentes patogênicos. Por serem necrófagas, atuam na decomposição de cadáveres e são úteis para a entomologia forense como indicadores na determinação do intervalo post mortem - IPM. Este trabalho visou conhecer a diversidade e a abundância das espécies em Campo Grande, Estado de Mato Grosso do Sul. As capturas foram realizadas semanalmente, com três armadilhas, utilizando isca de peixe deteriorado, durante o período de junho de 2008 a maio de 2009 em mata ciliar remanescente na fazenda experimental da Embrapa Gado de Corte. Foram consideradas as seguintes famílias de dípteros, seguidas pelo número de exemplares obtidos: Calliphoridae (105.334; Muscidae (27.999; Sarcophagidae (21

  8. New insight in the epidemiology of avian botulism outbreaks: necrophagous flies as vectors of Clostridium botulinum type C/D.

    Science.gov (United States)

    Anza, Ibone; Vidal, Dolors; Mateo, Rafael

    2014-12-01

    Avian botulism outbreaks spread through the bird carcass-maggot cycle, in which Clostridium botulinum and blowflies interact to ensure their reproduction in a mutualistic relationship where neurotoxin/spore-bearing maggot is one of the keystones. Here we investigated the hypothesis that adult blowflies may also play a significant role in botulism outbreaks by carrying C. botulinum cells between carcasses. We carried out a field experiment placing bird carcasses free of C. botulinum type C/D in containers only accessible to necrophagous flying insects in wetlands where avian botulism outbreaks were occurring and in control sites. Additionally, we performed laboratory trials to evaluate if blowflies may carry C. botulinum type C/D and for how long. Maggots bearing C. botulinum type C/D developed in 27.5% of carcasses placed in wetlands during botulism outbreaks. Calliphoridae flies in laboratory trials were able to transfer C. botulinum between two points and excreted it in their spots for up to 24 h after an infective feeding. Our results confirm that adult necrophagous flies play a role in the spreading of botulism outbreaks, which have implications in the epidemiology of this disease.

  9. Insects associated with the composting process of solid urban waste separated at the source

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    Gladis Estela Morales

    2010-01-01

    Full Text Available Sarcosaprophagous macroinvertebrates (earthworms, termites and a number of Diptera larvae enhance changes in the physical and chemical properties of organic matter during degradation and stabilization processes in composting, causing a decrease in the molecular weights of compounds. This activity makes these organisms excellent recyclers of organic matter. This article evaluates the succession of insects associated with the decomposition of solid urban waste separated at the source. The study was carried out in the city of Medellin, Colombia. A total of 11,732 individuals were determined, belonging to the classes Insecta and Arachnida. Species of three orders of Insecta were identified, Diptera, Coleoptera and Hymenoptera. Diptera corresponding to 98.5% of the total, was the most abundant and diverse group, with 16 families (Calliphoridae, Drosophilidae, Psychodidae, Fanniidae, Muscidae, Milichiidae, Ulidiidae, Scatopsidae, Sepsidae, Sphaeroceridae, Heleomyzidae, Stratiomyidae, Syrphidae, Phoridae, Tephritidae and Curtonotidae followed by Coleoptera with five families (Carabidae, Staphylinidae, Ptiliidae, Hydrophilidae and Phalacaridae. Three stages were observed during the composting process, allowing species associated with each stage to be identified. Other species were also present throughout the whole process. In terms of number of species, Diptera was the most important group observed, particularly Ornidia obesa, considered a highly invasive species, and Hermetia illuscens, both reported as beneficial for decomposition of organic matter.

  10. Characterization of necrophagus entomofauna in a typical agricultural area in Emilia-Romagna region (Northern Italy

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    M. Calzolari

    2014-04-01

    Full Text Available This study presents a checklist of Dipterans and Coleopterans accountable for carrion decay in the Po Valley (Italy, a contribution to the Forensic Entomology knowledge in Italy. Insects colonizing two pig carcasses in an agricultural area in Mezzani municipality (Parma, Northern Italy were sampled by pitfall traps and original Malaise-like traps, which allowed the sampling of a very relevant number of flying insects. A checklist of 57 taxa was obtained, of which 26 were considered of forensic importance. For the latter the arrival time of adult specimens on the carcasses was recorded, as an important parameter in minimum post mortem interval estimation. Dipterans (6141 specimens were the most common insects; the fastest specimens to detect and colonize the carcass belonged to the Calliphoridae family, while Fanniidae and Muscidae infested the carrion until completion of the skeletal stage. Coleopterans appeared later (308 adult specimens and 114 larvae were captured. Staphylinidae, Dermestidae, and Histeridae were the most common coleopterans sampled in this study.

  11. Patterns of diversity and abundance of carrion insect assemblages in the Natural Park "Hoces del Río Riaza" (central Spain).

    Science.gov (United States)

    Baz, Arturo; Cifrián, Blanca; Martín-Vega, Daniel

    2014-01-01

    The patterns of diversity and abundance of the carrion insect species in the different habitats of the Natural Park "Hoces del Río Riaza" (central Spain) were studied with the use of carrion-baited traps. Representativeness of the inventories was assessed with the calculation of randomized species richness curves and nonparametric estimators. Coleoptera families, Silphidae and Dermestidae, and Diptera families, Calliphoridae and Muscidae, were dominant in every sampling habitat, but differences in the patterns of diversity and abundance were found. Lusitanian oakwood and riparian forest were the most diverse habitats with high abundance of saprophagous species, whereas more open (i.e., exposed to continuous sunlight during the day) habitats showed lower diversity values and a different species composition and distribution of species abundance, favoring thermophilous species and necrophagous species with high tolerance to different environmental conditions. Differences in the bioclimatical features of the sampled habitats are suggested to explain the composition and diversity of the carrion insect assemblages in different environments.

  12. Cochliomyia Homnivorax in an advanced stage in the oral cavity

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    Layla Gomes

    Full Text Available Myiasis is characterized by the invasion of body or cavity tissues of live animals by larva. It is most frequently observed in underdeveloped and tropical countries, but there are cases described worldwide. Conventional treatment consists of mechanical removal of the larvae, one by one, which is a painful, embarrassing and repugnant process, both for the professional and patient. Although it is not considered rare, it has been observed that the dental professional has little knowledge for the diagnosis and treatment of this pathology. For this reason, this study reports a case of oral myiasis at an advanced stage, which affected a nine-year-old patient, treated at a medical-dental clinic. Diagnosis was based on the visual presence of Cochliomyia homnivorax larvae, diptera of the Calliphoridae family, which were between the second and third stages of development. Predisposing factors, such as the lack of information, malnutrition, poor oral hygiene, preexistenceof oral lesions and severe halitosis have a decisive influence in the appearance and progression of oral myiasis. A brief literaturereview was also conducted, in which this pathology was discussed, including the importance of early clinical diagnosis, its etiology, possible associations with other pathologies, and different types of treatment.

  13. Genetic approaches for studying myiasis-causing flies: molecular markers and mitochondrial genomics.

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    de Azeredo-Espin, Ana Maria Lima; Lessinger, Ana Cláudia

    2006-01-01

    "Myiasis-causing flies" is a generic term that includes species from numerous dipteran families, mainly Calliphoridae and Oestridae, of which blowflies, screwworm flies and botflies are among the most important. This group of flies is characterized by the ability of their larvae to develop in animal flesh. When the host is a live vertebrate, such parasitism by dipterous larvae is known as primary myiasis. Myiasis-causing flies can be classified as saprophagous (free-living species), facultative or obligate parasites. Many of these flies are of great medical and veterinary importance in Brazil because of their role as key livestock insect-pests and vectors of pathogens, in addition to being considered important legal evidence in forensic entomology. The characterization of myiasis-causing flies using molecular markers to study mtDNA (by RFLP) and nuclear DNA (by RAPD and microsatellite) has been used to identify the evolutionary mechanisms responsible for specific patterns of genetic variability. These approaches have been successfully used to analyze the population structures of the New World screwworm fly Cochliomyia hominivorax and the botfly Dermatobia hominis. In this review, various aspects of the organization, evolution and potential applications of the mitochondrial genome of myiasis-causing flies in Brazil, and the analysis of nuclear markers in genetic studies of populations, are discussed.

  14. DNA typing of Calliphorids collected from human corpses in Malaysia.

    Science.gov (United States)

    Kavitha, R; Tan, T C; Lee, H L; Nazni, W A; Sofian-Azirun, M

    2013-03-01

    Estimation of post-mortem interval (PMI) is crucial for time of death determination. The advent of DNA-based identification techniques forensic entomology saw the beginning of a proliferation of molecular studies into forensically important Calliphoridae (Diptera). The use of DNA to characterise morphologically indistinguishable immature calliphorids was recognised as a valuable molecular tool with enormous practical utility. The local entomofauna in most cases is important for the examination of entomological evidences. The survey of the local entomofauna has become a fundamental first step in forensic entomological studies, because different geographical distributions, seasonal and environmental factors may influence the decomposition process and the occurrence of different insect species on corpses. In this study, calliphorids were collected from 13 human corpses recovered from indoors, outdoors and aquatic conditions during the post-mortem examination by pathologists from the government hospitals in Malaysia. Only two species, Chrysomya megacephala and Chrysomya rufifacies were recovered from human corpses. DNA sequencing was performed to study the mitochondrial encoded COI gene and to evaluate the suitability of the 1300 base pairs of COI fragments for identification of blow fly species collected from real crime scene. The COI gene from blow fly specimens were sequenced and deposited in GenBank to expand local databases. The sequenced COI gene was useful in identifying calliphorids retrieved from human corpses.

  15. Fauna and abundance of medically important flies of Muscidae and Fanniidae (Diptera) in Tehran, Iran

    Institute of Scientific and Technical Information of China (English)

    MehdiKhoobdel; BehrozDavari

    2011-01-01

    Objective:To determine the faunal diversity of Muscidae and Fanniidae flies in Tehran, Iran. Methods:A net-capturing by direct observing method and fly trap were used to capture adult flies. To determine the fauna of flies in different habitats, 4 biotopes including corpse (human, birds, livestock), garbage and decaying organic matters, animal carcasses and human indoor habitat were selected. Big hashing nets (95 cm in diameter) have been used for adult flies capture in these biotopes. Results: In this study, totally 2 418 adult flies from 8 families including Muscidae, Fanniidae, Calliphoridae, Sarcophagidae, Tachinidae, Syrphidae, Anthomyiidae and Conopidae were captured. Among these, 1 279 belong to Muscidae and Fanniidae families. Four genera and 5 medically important species captured from two above mentioned families. Conclusions:Based on scientific documentation, 2 species of Muscina stabulans (M. stabulans) and Fannia scalaris (F. scalaris) are reported for the first time in Iran. However M. stabulans is a cosmopolitan species and its presence in Iran was probable.

  16. Large-scale mitogenomics enables insights into Schizophora (Diptera) radiation and population diversity.

    Science.gov (United States)

    Junqueira, Ana Carolina M; Azeredo-Espin, Ana Maria L; Paulo, Daniel F; Marinho, Marco Antonio T; Tomsho, Lynn P; Drautz-Moses, Daniela I; Purbojati, Rikky W; Ratan, Aakrosh; Schuster, Stephan C

    2016-02-25

    True flies are insects of the order Diptera and encompass one of the most diverse groups of animals on Earth. Within dipterans, Schizophora represents a recent radiation of insects that was used as a model to develop a pipeline for generating complete mitogenomes using various sequencing platforms and strategies. 91 mitogenomes from 32 different species were sequenced and assembled with high fidelity, using amplicon, whole genome shotgun or single molecule sequencing approaches. Based on the novel mitogenomes, we estimate the origin of Schizophora within the Cretaceous-Paleogene (K-Pg) boundary, about 68.3 Ma. Detailed analyses of the blowfly family (Calliphoridae) place its origin at 22 Ma, concomitant with the radiation of grazing mammals. The emergence of ectoparasitism within calliphorids was dated 6.95 Ma for the screwworm fly and 2.3 Ma for the Australian sheep blowfly. Varying population histories were observed for the blowfly Chrysomya megacephala and the housefly Musca domestica samples in our dataset. Whereas blowflies (n = 50) appear to have undergone selective sweeps and/or severe bottlenecks in the New World, houseflies (n = 14) display variation among populations from different zoogeographical zones and low levels of gene flow. The reported high-throughput mitogenomics approach for insects enables new insights into schizophoran diversity and population history of flies.

  17. [Dipterans of forensic interest in two vegetation profiles of cerrado in Uberlândia, State of Minas Gerais, Brazil].

    Science.gov (United States)

    Rosa, Thiago A; Babata, Micaela L Y; De Souza, Carina M; De Sousa, Danielle; De Mello-Patiu, Cátia A; Mendes, Júlio

    2009-01-01

    Carrion breeding insects may be of great utility in crimes briefing. Studies on forensic entomology are restricted to few localities in Brazil and very few of them were done in the cerrado vegetation (a type of Savannah) until now. This work aimed to survey the main species of dipterans associated with the decomposition process of Sus scrofa and the insect succession pattern in carcasses in the Cerrado. The study was carried out in the dry and humid season of the year, in two cerrado vegetation profiles in Uberlândia, MG. Two pig carcasses were placed in each vegetation profile and time period of the year. The decomposition process was slower and insects were more abundant in the dry season. Differences in temperature, air humidity and pluvial precipitation seemed to be the main physical factors responsible for the differences in the time of decomposition of the pig carcasses between the two seasons. Fourteen species of dipterans (59,467 specimens) bred in the decomposing carcasses. Chrysomya albiceps (Wiedemann) was the species more abundantly breed in the two periods. The other species that breed in the carcasses were: C. putoria (Wiedemann), Hemilucilia segmentaria(Fabricius), Lucilia eximia(Wiedemann) (Calliphoridae), Peckia (Pattonella) intermutans(Walker), Peckia (Squamatoides) trivitatta(Curran), Sarcodexia lambens(Wiedemann) (Sarcophagidae), Musca domestica(L.), Ophyra aenescens(Wiedemann), Stomoxys calcitransL. (Muscidae), Fannia pusio(Wiedemann), Fannia sp. (Fanniidae), Hermetia illuscens L. (Stratiomyidae) and Phoridae sp. The Cerrado of this region presents many dipterans potential forensic indicators.

  18. Differential Diptera succession patterns onto partially burned and unburned pig carrion in southeastern Brazil

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    J Oliveira-Costa

    Full Text Available In the present contribution we compared the entomological succession pattern of a burned carcass with that of an unburned one. For that, we used domestic pig carcasses and focused on Calliphoridae, Muscidae and Sarcophagidae flies, because they are the ones most commonly used in Postmortem Interval estimates. Adult and immature flies were collected daily. A total of 27 species and 2,498 specimens were collected, 1,295 specimens of 26 species from the partially burned carcass and 1,203 specimens of 22 species from the control carcass (unburned. The species composition in the two samples differed, and the results of the similarity measures were 0.875 by Sorensen and 0.756 by Bray-Curtis index. The results obtained for both carcasses also differ with respect to the decomposition process, indicating that the post mortem interval would be underestimated if the entomological succession pattern observed for a carcass under normal conditions was applied to a carbonized carcass.

  19. Indoor decomposition study in Malaysia with special reference to the scuttle flies (Diptera: Phoridae

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    Raja M. Zuha

    2016-09-01

    Full Text Available Scuttle flies (Diptera: Phoridae are a diversified insect group of forensic importance. Their frequent presence on human corpses indoors and in concealed environments can be the sole indicators to estimate the minimum post mortem interval (PMImin. However, bionomics of scuttle flies on decomposing animal carcasses are rarely documented indoors. The objective of this research is to observe and document the occurrence of scuttle flies on decomposing animal carcass placed inside a portable cabin maintained at room temperature (≈25.0 °C in Bangi, Malaysia. This study was conducted in two rounds for a period of 40-day each and samplings were carried out in different intervals. Adult scuttle flies were aspirated directly from the carcass and preserved in 70% ethanol. Their larvae and pupae were reared until adult stage to facilitate identification. Megaselia scalaris (Loew, Megaselia spiracularis (Schmitz and Dohrniphora cornuta (Bigot were the scuttle flies found on the carcasses with M. scalaris being the earliest and dominant to colonize the body. This cosmopolitan species proved to be the best indicator to estimate PMImin indoor but in the increased presence of other fly species, it might be relegated to a secondary role. The scuttle flies were also found to coexist with other dipterans of forensic importance in an indoor environment, mainly Chrysomya megacephala (Fabricius (Diptera: Calliphoridae. This information expands the knowledge on the bionomics of scuttle flies on decomposing animal remains indoors.

  20. A mathematical model applied for assisting the estimation of PMI in a case of forensic importance. First record of Conicera similis (Diptera: Phoridae) in a corpse.

    Science.gov (United States)

    García-Rojo, A M; Martínez-Sánchez, A; López, R; García de la Vega, J M; Rica, M; González, M; Disney, R H L

    2013-09-10

    We present a forensic case associated with skeletonized human remains found inside a cistern in a coastal town located in the eastern Iberian Peninsula (Valencian Regional Government, Spain). In order to analyse the particular environmental conditions that occurred during oviposition and development of the collected insects, estimated temperatures at the crime scene were calculated by a predictive mathematical model. This model analyses the correlation between the variability of the internal temperature depending on the variability of the external ones. The amplitude of the temperature oscillations inside the tank and the containment of the enclosure is reduced by the presence of water. Such variation occurred within about 2h due to the time required for heat exchange. The differential equations employed to model differences between outdoor and indoor temperatures were an essential tool which let us estimate the post-mortem interval (PMI) that was carried out by the study of the insect succession and the development time of the collected Diptera specimens under the adjusted temperatures. The presence of live larvae and pupae of Sarcophagidae and empty pupae of Calliphoridae, Sarcophagidae, Fanniidae, Muscidae, Phoridae and Piophilidae and the decomposition stage suggested the possibility that the remains were in the tank at least a year. We highlight the absence of Calliphora and Lucilia spp., and the first occurrence of the phorid Conicera similis in a human cadaver among the entomological evidence. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  1. EFFECTS OF THALLIUM ON THE LARVAL DEVELOPMENT OF LUCILIA SERICATA MEIGEN 1826 AND PMI ESTIMATION

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    Arif Gökhan BAŞARAN

    2011-08-01

    Full Text Available Determination of larval growth rate of and forensic analysis of the age of Calliphoridae larvae on a corpse are useful evidence in legal investigations for the estimation of exact death time and time duration after death; post mortem interval. However many factors, such as temperature, tissue type and contamination of drugs and toxins, effect larval development of blow fly larvae and consequently theestimation of post mortem interval. The present study examined the larval growth rate of a forensically important blow fly species, Lucilia sericata Meigen 1826 in different concentrations (0,12; 0,25; 0,50; 1 and 2 μg/g of toxic heavy metal Thallium under controlled laboratory conditions. Body length and weight, death ratio of larvae and pupa between experimental and control groups were compared. Results demonstrated that the development rate of larvae between uncontaminated and contaminated diets varies significantly. In short, they molted later, reached maximum length more slowly and sometimesproduced significantly smaller pupae in contaminated food source. These results emphasized that the importance of determining the contamination rate of toxins in tissue for the forensic entomologist,while using development rates from standard curves based on larvae fed non-contaminated mediums.

  2. Chronobiological studies on body search, oviposition and emergence of Megaselia scalaris (Diptera, Phoridae) in controlled conditions.

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    Bostock, Esta; Green, Edward W; Kyriacou, Charalambos P; Vanin, Stefano

    2017-03-18

    Circadian clocks have evolved to synchronize physiology, metabolism and behaviour to the 24-h geophysical cycles of the Earth. Understanding the circadian clock mechanism could play an important role in forensic entomology because it temporally gates behaviour such as locomotor activities, feeding, mating, egg laying and adult emergence which could provide useful information for crime reconstruction. The scuttle fly Megaselia scalaris colonises both exposed and buried bodies, whether indoors or outdoors. Locomotor activity, oviposition and adult emergence of this species have been investigated using technologies employed in many previous Drosophila circadian studies. The results reported here clearly highlight the underlying role of the circadian clock in regulating the behaviour of males and females of M. scalaris, and show the role of light as a "zeitgeber" for clock resetting. In contrast to Calliphoridae, M. scalaris can reach the oviposition site and lay eggs in darkness both during the day and the night, although the number of ovipositing females is lower under subjective darkness. The number of eggs laid shows a clear circadian rhythm with much higher numbers laid during the day than during night or subjective night. In conclusion, locomotor activity and oviposition rate of M. scalaris is under circadian clock control with significant forensic implications.

  3. Field calibration of blowfly-derived DNA against traditional methods for assessing mammal diversity in tropical forests.

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    Lee, Ping-Shin; Gan, Han Ming; Clements, Gopalasamy Reuben; Wilson, John-James

    2016-11-01

    Mammal diversity assessments based on DNA derived from invertebrates have been suggested as alternatives to assessments based on traditional methods; however, no study has field-tested both approaches simultaneously. In Peninsular Malaysia, we calibrated the performance of mammal DNA derived from blowflies (Diptera: Calliphoridae) against traditional methods used to detect species. We first compared five methods (cage trapping, mist netting, hair trapping, scat collection, and blowfly-derived DNA) in a forest reserve with no recent reports of megafauna. Blowfly-derived DNA and mist netting detected the joint highest number of species (n = 6). Only one species was detected by multiple methods. Compared to the other methods, blowfly-derived DNA detected both volant and non-volant species. In another forest reserve, rich in megafauna, we calibrated blowfly-derived DNA against camera traps. Blowfly-derived DNA detected more species (n = 11) than camera traps (n = 9), with only one species detected by both methods. The rarefaction curve indicated that blowfly-derived DNA would continue to detect more species with greater sampling effort. With further calibration, blowfly-derived DNA may join the list of traditional field methods. Areas for further investigation include blowfly feeding and dispersal biology, primer biases, and the assembly of a comprehensive and taxonomically-consistent DNA barcode reference library.

  4. Primer registro de artropodofauna cadavérica en sustratos humanos y animales en San Juan, Argentina

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    Fernando H. ABALLAY

    2008-01-01

    Full Text Available En el presente trabajo se estudiaron los artrópodos carroñeros que acudieron a cadáveres de vertebrados al aire libre en la provincia de San Juan, Argentina. El objetivo fue inventariar la composición específica de la artropodofauna cadavérica, asociada a diferentes sustratos de vertebrados en descomposición. Se colectaron muestras de artrópodos sobre restos animales y humanos en condiciones de campo y sobre cadáveres de cerdos domésticos colocados al aire libre bajo condiciones controladas. Se registraron, por primera vez para la provincia de San Juan, 40 especies de artropodofauna tanatológica incluidas en cuatro órdenes y 15 familias. Se incorpora, como primera cita para la fauna forense argentina, un necrófago: Megelenophorus americanus Lacordaire (Coleoptera: Tenebrionidae, y tres necrófilas: Polybia ruficeps Schrottky (Hymenoptera: Vespidae, Pheidole bergi Mayr (Hymenoptera, Formicidae, Myrmicinae y Ectatomma brunneum Smith (Hymenoptera, Formicidae, Ponerinae. Se citan 18 especies necrófagas, 18 necrófilas, una omnívora y seis oportunistas sobre siete diferentes sustratos cadavéricos de vertebrados. Se brindan nuevos registros de distribución de 18 especies de insectos. Se confirma la estacionalidad invernal de Callíphora vicina Robineau-Desvoidy (Diptera: Calliphoridae.

  5. Large-scale mitogenomics enables insights into Schizophora (Diptera) radiation and population diversity

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    Junqueira, Ana Carolina M.; Azeredo-Espin, Ana Maria L.; Paulo, Daniel F.; Marinho, Marco Antonio T.; Tomsho, Lynn P.; Drautz-Moses, Daniela I.; Purbojati, Rikky W.; Ratan, Aakrosh; Schuster, Stephan C.

    2016-01-01

    True flies are insects of the order Diptera and encompass one of the most diverse groups of animals on Earth. Within dipterans, Schizophora represents a recent radiation of insects that was used as a model to develop a pipeline for generating complete mitogenomes using various sequencing platforms and strategies. 91 mitogenomes from 32 different species were sequenced and assembled with high fidelity, using amplicon, whole genome shotgun or single molecule sequencing approaches. Based on the novel mitogenomes, we estimate the origin of Schizophora within the Cretaceous-Paleogene (K-Pg) boundary, about 68.3 Ma. Detailed analyses of the blowfly family (Calliphoridae) place its origin at 22 Ma, concomitant with the radiation of grazing mammals. The emergence of ectoparasitism within calliphorids was dated 6.95 Ma for the screwworm fly and 2.3 Ma for the Australian sheep blowfly. Varying population histories were observed for the blowfly Chrysomya megacephala and the housefly Musca domestica samples in our dataset. Whereas blowflies (n = 50) appear to have undergone selective sweeps and/or severe bottlenecks in the New World, houseflies (n = 14) display variation among populations from different zoogeographical zones and low levels of gene flow. The reported high-throughput mitogenomics approach for insects enables new insights into schizophoran diversity and population history of flies. PMID:26912394

  6. Alimentary Canal of the Adult Blow Fly, Chrysomya megacephala (F. (Diptera: Calliphoridae—Part I: Ultrastructure of Salivary Glands

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    Worachote Boonsriwong

    2012-01-01

    Full Text Available The salivary gland ultrastructure of the adult male blow fly, Chrysomya megacephala (F. (Diptera: Calliphoridae, was investigated at the ultrastructural level using light microscopy (LM, scanning electron microscopy (SEM, and transmission electron microscopy (TEM. The salivary glands are paired structures composed of a single median deferent duct bifurcated into two long, narrow efferent ducts connected to the coiled tubular glands. The SEM image of the gland surface revealed that the basal lamina is relatively smooth in general, but the whole surface appeared as a trace of rough swollen insertion by intense tracheal ramification. Ultrastructurally, the salivary gland is enclosed within the basal lamina, and interdigitation cytoplasmic extensions were apparent between the adjacent gland cells. The basement membrane appeared infoldings that is similar to the complex of the labyrinth channel. The cytoplasm characteristic of the gland revealed high activity, based on the abundance of noticeable secretory granules, either singly or in an aggregated reservoir. In addition, mitochondria were found to intersperse among rich parallel of arrays rough endoplasmic reticulum. Thick cuticle, which was well-delineated and electron dense, apically lined the gland compartments, with discontinuity of the double-layer cuticle revealing a trace of secretion discharged into the lumen. Gross anatomy of the adult salivary gland was markedly different from that of the third instar of the same species, and structural dissimilarity is discussed briefly.

  7. Survey of the Synanthropic Flies Associated with Human Habitations in Ubon Ratchathani Province of Northeast Thailand

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    Tarinee Chaiwong

    2012-01-01

    Full Text Available Synanthropic fly surveys were performed to determine the species composition and abundance in Ubon Ratchathani province in Northeast Thailand. Adult fly collections were conducted in various human habitations from two districts—Muang Ubon Ratchathani and Warinchamrap, at fresh-food markets, garbage piles, restaurants, school cafeterias, and rice paddy fields. Customized reconstructable funnel fly traps baited with 250 g of 1-day tainted beef were used for fly collections from September 2010–February 2011. A total of 3,262 flies were captured, primarily consisting of three families including: Calliphoridae (6 species, Muscidae (3 species, and Sarcophagidae (11 species. The blow fly, Chrysomya megacephala, and the house fly, Musca domestica, were the dominant species collected from both districts at all collection sites. C. megacephala was predominant in paddy fields, restaurants and garbage piles, while M. domestica was numerically dominant in fresh-food markets and school cafeterias. The current survey identified various species of synanthropic flies with close associations to humans and with the ability to transmit human pathogens in Ubon Ratchathani province; providing crucial information that may be used for developing control and sanitation management plans in this particular area.

  8. Strengthen forensic entomology in court--the need for data exploration and the validation of a generalised additive mixed model.

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    Baqué, Michèle; Amendt, Jens

    2013-01-01

    Developmental data of juvenile blow flies (Diptera: Calliphoridae) are typically used to calculate the age of immature stages found on or around a corpse and thus to estimate a minimum post-mortem interval (PMI(min)). However, many of those data sets don't take into account that immature blow flies grow in a non-linear fashion. Linear models do not supply a sufficient reliability on age estimates and may even lead to an erroneous determination of the PMI(min). According to the Daubert standard and the need for improvements in forensic science, new statistic tools like smoothing methods and mixed models allow the modelling of non-linear relationships and expand the field of statistical analyses. The present study introduces into the background and application of these statistical techniques by analysing a model which describes the development of the forensically important blow fly Calliphora vicina at different temperatures. The comparison of three statistical methods (linear regression, generalised additive modelling and generalised additive mixed modelling) clearly demonstrates that only the latter provided regression parameters that reflect the data adequately. We focus explicitly on both the exploration of the data--to assure their quality and to show the importance of checking it carefully prior to conducting the statistical tests--and the validation of the resulting models. Hence, we present a common method for evaluating and testing forensic entomological data sets by using for the first time generalised additive mixed models.

  9. [Forensic entomology].

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    Açikgöz, Halide Nihal

    2010-01-01

    Odour of the animal or human corpses immediately after death is very attractive for insects and other invertebrates. Blue and green bottle flies from the Calliphoridae family are the first colonizers of cadaver and immediately later necrophagous Diptera from the Sarcophagidae family settle on the same corpse. It is essential to determine the time past after death for elucidating the event in case of the homicide or suspicious death, and it is directly proportional to the post mortem interval expected time, which is based upon the speed of the larval growth. In this article, we purposed to stress the special interest of forensic entomology for the scientists who will apply this science in their forensic researches and case studies, and also to provide information to our judges, prosecutors and law enforcement agents in order to consider the entomological samples to be reliable and applicable evidences as biological stains and hairs. We are of the opinion that if any forensic entomologist is called to the crime scene or if the evidences are collected and then delivered to an entomologist, the forensic cases will be elucidated faster and more accurately.

  10. Are the evidences of forensic entomology preserved in ethanol suitable for SEM studies?

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    López-Esclapez, Raquel; García, María-Dolores; Arnaldos, María-Isabel; Presa, Juan José; Ubero-Pascal, Nicolás

    2014-07-01

    In forensic practice, the use of arthropod evidences to estimate the postmortem interval is a very good approach when the elapsed time from death is long, but it requires the correct identification of the specimens. This is a crucial step, not always easy to achieve, in particular when dealing with immature specimens. In this case, scanning electronic microscopy (SEM) can be useful, but the techniques used to preserve specimens in forensic practice are usually different from those used to prepare specimens for SEM studies. To determine whether forensic evidences preserving techniques are also compatible with SEM analysis, we have compared specimens of all the immature stages of Calliphora vicina Robineau-Desvoidy, 1830 (Diptera, Calliphoridae) preserved in 70% ethanol, with others prepared with aldehydic fixative techniques that are more appropriate for SEM studies. At the same time, two drying techniques have also been compared with both fixative techniques, the critical point drying and air-drying following with hexamethyldisilizane treatment (HMDS). Our results indicate that there are not basis against recommending the use of ethanol to preserve forensic entomological evidences and that both drying methods appear to offer good results for second and third instar larvae, although HMDS behaves better with eggs and pupae.

  11. Virtual forensic entomology: improving estimates of minimum post-mortem interval with 3D micro-computed tomography.

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    Richards, Cameron S; Simonsen, Thomas J; Abel, Richard L; Hall, Martin J R; Schwyn, Daniel A; Wicklein, Martina

    2012-07-10

    We demonstrate how micro-computed tomography (micro-CT) can be a powerful tool for describing internal and external morphological changes in Calliphora vicina (Diptera: Calliphoridae) during metamorphosis. Pupae were sampled during the 1st, 2nd, 3rd and 4th quarter of development after the onset of pupariation at 23 °C, and placed directly into 80% ethanol for preservation. In order to find the optimal contrast, four batches of pupae were treated differently: batch one was stained in 0.5M aqueous iodine for 1 day; two for 7 days; three was tagged with a radiopaque dye; four was left unstained (control). Pupae stained for 7d in iodine resulted in the best contrast micro-CT scans. The scans were of sufficiently high spatial resolution (17.2 μm) to visualise the internal morphology of developing pharate adults at all four ages. A combination of external and internal morphological characters was shown to have the potential to estimate the age of blowfly pupae with a higher degree of accuracy and precision than using external morphological characters alone. Age specific developmental characters are described. The technique could be used as a measure to estimate a minimum post-mortem interval in cases of suspicious death where pupae are the oldest stages of insect evidence collected.

  12. Forensic entomology cases in Thailand: a review of cases from 2000 to 2006.

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    Sukontason, Kom; Narongchai, Paitoon; Kanchai, Chaturong; Vichairat, Karnda; Sribanditmongkol, Pongruk; Bhoopat, Tanin; Kurahashi, Hiromu; Chockjamsai, Manoch; Piangjai, Somsak; Bunchu, Nophawan; Vongvivach, Somsak; Samai, Wirachai; Chaiwong, Tarinee; Methanitikorn, Rungkanta; Ngern-Klun, Rachadawan; Sripakdee, Duanghatai; Boonsriwong, Worachote; Siriwattanarungsee, Sirisuda; Srimuangwong, Chaowakit; Hanterdsith, Boonsak; Chaiwan, Khankam; Srisuwan, Chalard; Upakut, Surasak; Moopayak, Kittikhun; Vogtsberger, Roy C; Olson, Jimmy K; Sukontason, Kabkaew L

    2007-10-01

    This paper presents and discusses 30 cases of cadavers that had been transferred for forensic entomology investigations to the Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, northern Thailand, from 2000 to 2006. Variable death scenes were determined, including forested area and suburban and urban outdoor and indoor environments. The fly specimens found in the corpses obtained were the most commonly of the blow fly of family Calliphoridae, and consisted of Chrysomya megacephala (F.), Chrysomya rufifacies (Macquart) Chrysomya villeneuvi Patton, Chrysomya nigripes Aubertin, Chrysomya bezziana Villeneuve, Chrysomya chani Kurahashi, Lucilia cuprina (Wiedemann), Hemipyrellia ligurriens (Wiedemann), and two unknown species. Flies of the family Muscidae [Hydrotaea spinigera Stein, Synthesiomyia nudiseta (Wulp)], Piophilidae [Piophila casei (L.)], Phoridae [Megaselia scalaris (Loew)], Sarcophagidae [Parasarcophaga ruficornis (F.) and three unknown species], and Stratiomyiidae (Sargus sp.) were also collected from these human remains. Larvae and adults of the beetle, Dermestes maculatus DeGeer (Coleoptera: Dermestidae), were also found in some cases. Chrysomya megacephala and C. rufifacies were the most common species found in the ecologically varied death scene habitats associated with both urban and forested areas, while C. nigripes was commonly discovered in forested places. S. nudiseta was collected only from corpses found in an indoor death scene.

  13. Pollination and mating systems of Apodanthaceae and the distribution of reproductive traits in parasitic angiosperms.

    Science.gov (United States)

    Bellot, Sidonie; Renner, Susanne S

    2013-06-01

    The most recent reviews of the reproductive biology and sexual systems of parasitic angiosperms were published 17 yr ago and reported that dioecy might be associated with parasitism. We use current knowledge on parasitic lineages and their sister groups, and data on the reproductive biology and sexual systems of Apodanthaceae, to readdress the question of possible trends in the reproductive biology of parasitic angiosperms. • Fieldwork in Zimbabwe and Iran produced data on the pollinators and sexual morph frequencies in two species of Apodanthaceae. Data on pollinators, dispersers, and sexual systems in parasites and their sister groups were compiled from the literature. • With the possible exception of some Viscaceae, most of the ca. 4500 parasitic angiosperms are animal-pollinated, and ca. 10% of parasites are dioecious, but the gain and loss of dioecy across angiosperms is too poorly known to infer a statistical correlation. The studied Apodanthaceae are dioecious and pollinated by nectar- or pollen-foraging Calliphoridae and other flies. • Sister group comparisons so far do not reveal any reproductive traits that evolved (or were lost) concomitant with a parasitic life style, but the lack of wind pollination suggests that this pollen vector may be maladaptive in parasites, perhaps because of host foliage or flowers borne close to the ground.

  14. A preliminary identification of insect successive wave in Egypt on control and zinc phosphide-intoxicated animals in different seasons

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    Marah Mohammad Abd El-Bar

    2016-09-01

    Full Text Available The presented study aimed primarily to document a baseline data of the decay process of rabbits and guinea pigs and their associated arthropod fauna, which are placed in an urban city: El Abbassyia, Cairo Governorate, Egypt, during winter and summer seasons, and to compare these data with the corresponding figure for zinc phosphide-intoxicated carrions. Generally, control rabbits and control guinea pigs were faster in their decay comparing the corresponding figure of the zinc phosphide–intoxicated group. A delay in colonization of insects was noticed either in the winter season for both groups, or additionally for the zinc phosphide groups. The associated insect fauna was represented in 6 orders, 20 families, and 36 genera and species. Necrophagous arthropods that supported decomposition of carcasses were mainly of orders Diptera and Coleoptera. Calliphoridae was the first insect family that colonized the different carcasses. The mean numbers of control immature dipterous maggots and similarly, the control coleopteran larvae significantly exceeded the corresponding mean numbers for the zinc phosphide-intoxicated groups in both winter and summer seasons in either rabbits or guinea pig groups. Moreover, the mean numbers of dipterous maggots or coleopteran larvae of rabbits significantly surpassed the corresponding figures for guinea pigs in both seasons. This study may add as a reference for the succession wave arthropod fauna in Cairo Governorate in winter and summer seasons. Moreover, it is the first record of the arthropod successive wave on zinc phosphide–intoxicated remains.

  15. Dipterofauna Associated with Sus scrofa Linné, 1758, Carcasses in Urban and Coastal Regions of São Paulo State, Brazil

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    Maria Luiza Cavallari

    2015-01-01

    Full Text Available Cadaverous entomofauna successions vary according to the region, environment, and climate, and such differences may occur within the same country due to seasonal variations. The present study aimed to analyze and compare the dipterofauna that visit or colonize carcasses in the urban and coastal areas of São Paulo, Brazil, during summer and winter seasons. Four swine (Sus scrofa Linné, 1758 carcasses of approximately 12 kg were used. The animals were previously euthanized and then placed in metal cages covered with a flight intercept trap (Shannon, modified. In total, 10,495 flies from 39 families were collected, with 15 species belonging to the Calliphoridae family, 14 species belonging to the Fanniidae family, 43 species belonging to the Muscidae family, and 22 species belonging to the Sarcophagidae family. Flies from these four families visited all carcasses; however, they did not show the highest visitation frequencies in all of the trials. Species variations occurred between the experiments that were performed at different locations and in different seasons. Furthermore, difference in the number of insects attracted to each stage of decomposition was observed. In addition to the four families highlighted above, the families Phoridae, Sepsidae, Otitidae, and Piophilidae were observed in all carcasses.

  16. A combined protocol for identification of maggots of forensic interest.

    Science.gov (United States)

    Tuccia, Fabiola; Giordani, Giorgia; Vanin, Stefano

    2016-07-01

    In Forensic Entomology the estimation of the age of insects is used for the estimation of the minimum post-mortem interval. As insect development is temperature dependent and species specific, a correct species identification is therefore fundamental. In the majority of cases the molecular identification is based on a destructive approach. In this paper a working protocol for molecular identification of fly larvae without affecting the anatomical characters used for morphological identification is presented. The suggested technique allows the preservation of the larval exoskeleton and of the unused soft tissues in the same vial allowing a repetition of both the morphological and molecular identification and reducing the risk of loss of the evidence. This method also allows the possibility of measuring the size of the specimens before their morphological and biomolecular characterization. In order to demonstrate that this technique can be applied on maggots of a large spectrum of dimensions it has been tested and validated using larvae of different size from ~1.7-1.3cm [Calliphora vomitoria and Lucilia sericata (Diptera: Calliphoridae)] to ~10-6.5mm [Musca domestica (Diptera: Muscidae) and Megaselia scalaris (Diptera: Phoridae)]. The importance of a unique identifier and of a complete database with all the specimen information (origin, sample size, identification, etc.) is also discussed.

  17. COII ”long fragment” reliability in characterisation and classification of forensically important flies

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    Sanaa M. Aly

    2017-01-01

    Full Text Available Introduction : Molecular identification of collected flies is important in forensic entomological analysis guided with accurate evaluation of the chosen genetic marker. The selected mitochondrial DNA segments can be used to properly identify species. The aim of the present study was to determine the reliability of the 635-bp-long cytochrome oxidase II gene (COII in identification of forensically important flies. Material and methods: Forty-two specimens belonging to 11 species ( Calliphoridae: Chrysomya albiceps , C. rufifacies , C. megacephala , Lucilia sericata , L. cuprina ; Sarcophagidae: Sarcophaga carnaria , S. dux , S. albiceps , Wohlfahrtia nuba ; Muscidae: Musca domestica , M. autumnalis were analysed. The selected marker was amplified using PCR followed by sequencing. Nucleotide sequence divergences were calculated using the K2P (Kimura two-parameter distance model, and a NJ (neighbour-joining phylogenetic tree was constructed. Results : All examined specimens were assigned to the correct species, formed distinct monophyletic clades and ordered in accordance with their taxonomic classification. Intraspecific variation ranged from 0 to 1% and interspecific variation occurred between 2 and 20%. Conclusions : The 635-bp-long COII marker is suitable for clear differentiation and identification of forensically relevant flies.

  18. Keragaman Jenis dan Prevalensi Lalat Pasar Tradisional di Kota Bogor (DIVERSITY AND PREVALENCE OF FLIES AT TRADITIONAL MARKETS IN BOGOR CITY

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    Puguh Wahyudi

    2016-01-01

    Full Text Available Bogor city is one of the greater Jabodetabek area which has a fairly high growth of the modern market.This should not shift the role of traditional market, if accompanied with an increase in the number andquality of traditional markets, among others by controlling infestations of flies on the market that can bea vector of various diseases.This research was conducted to identify the diversity and infestation of fliesspesies in five old Bogor traditional markets. The flies were collected using insect nets and then killed withchloroform to count and identification purposes. Measuring the prevalence of flies infestation in eachmarket were using sticky fly paper on block sale of meat, fish and outside market environment. Therewere ten fly spesies belong to four main families that Calliphoridae (C. megacephala, C. saffranea, C.rufifacies, and Lucilia sericata, Muscidae (M. domestica, M. conducens, and M. fasciata, Sarcophagidae (S.haemorroidalis, and S. fuscicauda, and Drosophilidae (Drosophila repleta. The others three families werePhoridae, Anthomyiidae, and Syrphidae. Fly diversity index on each markets were 1.203 (Bogor Market,1.038 (Sukasari Market, 2.678 (Anyar Market, 1.017 (Jambu Dua Market, and 1.618 (Gunung BatuMarket. Measurement of Calliphorid flies infestations as an indicator of the presence of litterdecomposition of organic material showed a high concentration in the market environment.These resultsillustrate the general environmental sanitation of traditional markets are bad.

  19. Temporal distribution of ten calyptrate dipteran species of medicolegal importance in Rio de Janeiro, Brazil

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    Rodrigo Rocha Barbosa

    2010-03-01

    Full Text Available Brazilian studies involving entomological succession patterns in carcasses have been used to describe the necrophagous entomofauna of a determined municipality or region with forensic objectives. Following the same objectives, an ecological study with 10 calyptrate dipterans was carried out during the winter of 2007 and the summer of 2008 in the metropolitan region of the municipality of Rio de Janeiro. The aim of this study was to describe several aspects of the phenology of these species in three neighbouring areas. Carcasses of three domestic pigs (Sus scrofa L. were used in each season as models for forensic and legal medicine investigations in the region. Temperature, relative humidity and rainfall were measured daily and their relations with population abundance of the colonising species and the decomposition stages were analysed. Ten fly species were recorded to be colonising the carcasses, five of which belonged to the Calliphoridae family, three to the Muscidae, one to the Fanniidae and one to the Sarcophagidae family. Data show preferences of these species for climatic season and decomposition stage, as well as for the studied area and suggest that short distances can significantly influence the abundance of some species.

  20. Influence of weather conditions on fly abundance and its implications for transmission of rabbit haemorrhagic disease virus in the North Island of New Zealand.

    Science.gov (United States)

    Henning, J; Schnitzler, F-R; Pfeiffer, D U; Davies, P

    2005-09-01

    Blowflies (Diptera: Calliphoridae) and flesh flies (Diptera: Sarcophagidae) are potential vectors of rabbit haemorrhagic disease virus (RHDV) in New Zealand. The associations between habitat and weather factors on the abundance of these flies were investigated. Between October 1999 and June 2001, flies were trapped on open pasture and in dense vegetation patches on farmland in the Himatangi area of the North Island. Five calliphorid species were trapped commonly at scrub edges and the most abundant sarcophagid, Oxysarcodexia varia Walker, was trapped mainly on open pasture. An abundance peak of O. varia was probably associated with the occurrence of a rabbit haemorrhagic disease (RHD) outbreak in the study area. Overall abundance of flies varied according to habitat and species, and species numbers differed between seasons and years. The all-day minimum temperature 3 weeks before trapping was a significant variable in all models of fly abundance, whereas average rainfall did not affect fly abundance. The all-day temperature range was significant only for O. varia. The influence of other climatic factors varied between fly species. Climate dependent variations in fly abundance may contribute to the risk of transmission of RHD, which occurred intermittently on the site during the study period.