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Sample records for calibrated diversity assay

  1. Calibrated user-friendly reverse transcriptase-PCR assay

    DEFF Research Database (Denmark)

    Bor, M V; Sørensen, B S; Rammer, P;

    1998-01-01

    We report a competitive reverse transcriptase-PCR (RT-PCR) assay and a calibrated user-friendly RT-PCR assay (CURT-PCR) for epidermal growth factor receptor (EGFR) mRNA. A calibrator was prepared from isolated rat liver RNA, and the amount of EGFR mRNA was determined by competitive RT-PCR. In CURT-PCR......, a calibration curve was developed by plotting the ratio between the amount of PCR product originating from the calibrator and the RNA internal standard vs the amount of EGFR mRNA present in the calibrator. A fixed amount of RNA internal standard was coamplified with the EGFR mRNA present in the...... calibrator or in the sample, using the same primer set. The primers were chosen in regions of the EGFR mRNA that show 100% homology between human, rat, and mouse. The amount of EGFR in the unknown samples was calculated from the calibration curve based on the ratio between PCR product originating from the...

  2. On the efficiency calibration of a drum waste assay system

    CERN Document Server

    Dinescu, L; Cazan, I L; Macrin, R; Caragheorgheopol, G; Rotarescu, G

    2002-01-01

    The efficiency calibration of a gamma spectroscopy waste assay system, constructed by IFIN-HH, was performed. The calibration technique was based on the assumption of a uniform distribution of the source activity in the drum and also a uniform sample matrix. A collimated detector (HPGe--20% relative efficiency) placed at 30 cm from the drum was used. The detection limit for sup 1 sup 3 sup 7 Cs and sup 6 sup 0 Co is approximately 45 Bq/kg for a sample of about 400 kg and a counting time of 10 min. A total measurement uncertainty of -70% to +40% was estimated.

  3. Development of a Calibration Strip for Immunochromatographic Assay Detection Systems.

    Science.gov (United States)

    Gao, Yue-Ming; Wei, Jian-Chong; Mak, Peng-Un; Vai, Mang-I; Du, Min; Pun, Sio-Hang

    2016-01-01

    With many benefits and applications, immunochromatographic (ICG) assay detection systems have been reported on a great deal. However, the existing research mainly focuses on increasing the dynamic detection range or application fields. Calibration of the detection system, which has a great influence on the detection accuracy, has not been addressed properly. In this context, this work develops a calibration strip for ICG assay photoelectric detection systems. An image of the test strip is captured by an image acquisition device, followed by performing a fuzzy c-means (FCM) clustering algorithm and maximin-distance algorithm for image segmentation. Additionally, experiments are conducted to find the best characteristic quantity. By analyzing the linear coefficient, an average value of hue (H) at 14 min is chosen as the characteristic quantity and the empirical formula between H and optical density (OD) value is established. Therefore, H, saturation (S), and value (V) are calculated by a number of selected OD values. Then, H, S, and V values are transferred to the RGB color space and a high-resolution printer is used to print the strip images on cellulose nitrate membranes. Finally, verification of the printed calibration strips is conducted by analyzing the linear correlation between OD and the spectral reflectance, which shows a good linear correlation (R² = 98.78%). PMID:27367694

  4. EPA Traceability Protocol for Assay and Certification of Gaseous Calibration Standards (2012 Revision)

    Science.gov (United States)

    In 1997, the U.S. Environmental Protection Agency (EPA) in Research Triangle Park, North Carolina, revised its 1993 version of its traceability protocol for the assay and certification of compressed gas and permeation-device calibration standards. The protocol allows producers o...

  5. Lyophilized standards for the calibration of real time PCR assay for hepatitis C virus RNA

    Institute of Scientific and Technical Information of China (English)

    WANG Lu-nan; WU Jian-min; DENG Wei; SHEN Zi-yu; CHEN Wen-xiang; LI Jin-ming

    2006-01-01

    Background Since October 1997, an international standard for hepatitis C virus (HCV) nucleic acid amplification technology assay, 96/790, has been available. We compared a series of lyophilized standards with known HCV RNA concentrations against the international standard in fluorescence quantitative PCR detection.Methods A series of lyophilized sera were calibrated by ROCHE COBAS AMPLICOR HCV Monitor test against the international standard and sent to various manufacturers to analyse the samples using their own kits.Then calibration curves from the series were compared with that obtained from the external standard calibration curve with the manufacture's series.Results The standard calibration curve with the series of lyophilized serum showed an excellent correlation(R2>0.98), slope and intercept that were similar to those from the manufacture's series. When the standard calibration curve from the series of lyophilized standards were used to define the values of the given sample,lower coefficients of variation between kits from different manufactures were obtained.Conclusion The results showed that the lyophilized standards could be used to setup the standard calibration curve for clinical HCV RNA quantitative PCR detection.

  6. Multiplex cytological profiling assay to measure diverse cellular states.

    Directory of Open Access Journals (Sweden)

    Sigrun M Gustafsdottir

    Full Text Available Computational methods for image-based profiling are under active development, but their success hinges on assays that can capture a wide range of phenotypes. We have developed a multiplex cytological profiling assay that "paints the cell" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects seven major cellular components. In a pilot screen of bioactive compounds, the assay detected a range of cellular phenotypes and it clustered compounds with similar annotated protein targets or chemical structure based on cytological profiles. The results demonstrate that the assay captures subtle patterns in the combination of morphological labels, thereby detecting the effects of chemical compounds even though their targets are not stained directly. This image-based assay provides an unbiased approach to characterize compound- and disease-associated cell states to support future probe discovery.

  7. New data concerning the efficiency calibration of a drum waste assay system

    International Nuclear Information System (INIS)

    The study is focused on the efficiency calibration of a gamma spectroscopy system for drum waste assay.The measurement of a radioactive drum waste is usually difficult because of its large volume, the varied distribution of the waste within the drum and its high self attenuation.To solve this problems, a complex calibration of the system is required. For this purpose, a calibration drum provided with seven tubes, placed at different distances from its center was used, the rest of the drum being filled with Portland cement. For the efficiency determination of a uniformly distributed source, a linear source of 152 Eu was used.The linear calibration source was introduced successively inside the seven tubes, the gamma spectra being recorded while the drum was translated and simultaneously rotated. Using the GENIE-PC software, the gamma-spectra were analyzed and the detection efficiencies for shell-sources were obtained. Using this efficiencies, the total response of the detector and the detection efficiency appropriate to a uniform volume source were calculated. For the efficiency determination of a non-homogenous source, additional measurements in the following geometries were made. First, with a 152 Eu point source placed in front of the detector, measured in all seven tubes, the drum being only rotated. Second, with the linear source of 152 Eu placed in front of the detector, measured in all seven tubes, only the drum being rotated. For each position the gamma spectra was recorded and the detection efficiency was calculated.The obtained values for efficiency were verified using GESPECOR software, which has been developed for the computation of the efficiency of Ge detectors for a wide class of measurement configurations, using Monte-Carlo method. (authors)

  8. Contactless vector network analysis using diversity calibration with capacitive and inductive coupled probes

    Directory of Open Access Journals (Sweden)

    T. Zelder

    2007-06-01

    Full Text Available Contactless vector network analysis based on a diversity calibration is investigated for the measurement of embedded devices in planar circuits. Conventional contactless measurement systems based on two probes for each measurement port have the disadvantage that the signal-to-noise system dynamics strongly depends on the distance between the contactless probes.

    In order to avoid a decrease in system dynamics a diversity based measurement system is presented. The measurement setup uses one inductive and two capacitive probes. As an inductive probe a half magnetic loop in combination with a broadband balun is introduced. In order to eliminate systematic errors from the measurement results a diversity calibration algorithm is presented. Simulation and measurement results for a one-port configuration are shown.

  9. Diversity in the applications of the single cell gel electrophoresis (comet) assay / Cristal Huysamen

    OpenAIRE

    Huysamen, Cristal

    2005-01-01

    The development of the single cell gel electrophoresis assay (Comet assay) as a powerful method for measuring DNA strand breakage and repair, has lead to a broader understanding of the impact of certain internal and external factors on DNA damage. This study describes the establishment of the Comet assay in our laboratory and its application in a diversity of studies. These studies include the monitoring of the effect of exercise on DNA damage and repair with the purpose of ...

  10. EPA Traceability Protocol for Assay and Certification of Gaseous Calibration Standards (EPA/600/R-12/531, May 2012)

    Science.gov (United States)

    In 1997, the U.S. Environmental Protection Agency (EPA) in Research Triangle Park, North Carolina, revised its 1993 version of its traceability protocol for the assay and certification of compressed gas and permeation-device calibration standards. The protocol allows producers of...

  11. Use of calibration standards and the correction for sample self-attenuation in gamma-ray nondestructive assay

    International Nuclear Information System (INIS)

    The efficient use of appropriate calibration standards and the correction for the attenuation of the gamma rays within an assay sample by the sample itself are two important and closely related subjects in gamma-ray nondestructive assay. Much research relating to those subjects has been done in the Nuclear Safeguards Research and Development program at the Los Alamos National Laboratory since 1970. This report brings together most of the significant results of that research. Also discussed are the nature of appropriate calibration standards and the necessary conditions on the composition, size, and shape of the samples to allow accurate assays. Procedures for determining the correction for the sample self-attenuation are described at length including both general principles and several specific useful cases. The most useful concept is that knowing the linear attenuation coefficient of the sample (which can usually be determined) and the size and shape of the sample and its position relative to the detector permits the computation of the correction factor for the self-attenuation. A major objective of the report is to explain how the procedures for determining the self-attenuation correction factor can be applied so that calibration standards can be entirely appropriate without being particularly similar, either physically or chemically, to the items to be assayed. This permits minimization of the number of standards required to assay items with a wide range of size, shape, and chemical composition. 17 references, 18 figures, 2 tables

  12. Comparison of a High-Resolution Melting Assay to Next-Generation Sequencing for Analysis of HIV Diversity

    OpenAIRE

    Matthew M Cousins; Ou, San-San; Wawer, Maria J.; Munshaw, Supriya; Swan, David; Magaret, Craig A.; Mullis, Caroline E.; Serwadda, David; Stephen F Porcella; Gray, Ronald H.; Thomas C Quinn; Donnell, Deborah; Susan H Eshleman; Redd, Andrew D.

    2012-01-01

    Next-generation sequencing (NGS) has recently been used for analysis of HIV diversity, but this method is labor-intensive, costly, and requires complex protocols for data analysis. We compared diversity measures obtained using NGS data to those obtained using a diversity assay based on high-resolution melting (HRM) of DNA duplexes. The HRM diversity assay provides a single numeric score that reflects the level of diversity in the region analyzed. HIV gag and env from individuals in Rakai, Uga...

  13. Collaborative study for the calibration of replacement batches for the heparin low-molecular-mass for assay biological reference preparation.

    Science.gov (United States)

    Terao, E; Daas, A

    2016-01-01

    The European Pharmacopoeia (Ph. Eur.) prescribes the control of the activity of low molecular mass heparins by assays for anti-Xa and anti-IIa activities (monograph 0828), using a reference standard calibrated in International Units (IU). An international collaborative study coded BSP133 was launched in the framework of the Biological Standardisation Programme (BSP) run under the aegis of the Council of Europe and the European Commission to calibrate replacement batches for the dwindling stocks of the Heparin low-molecular-mass for assay Biological Reference Preparation (BRP) batch 8. Thirteen official medicines control and manufacturers laboratories from European and non-European countries took part in this study to calibrate two freeze-dried candidate batches against the 3rd International Standard (IS) for heparin, low molecular weight (11/176; 3rd IS). The Heparin low-molecular-mass for assay BRP (batch 8) was also included in the test panel to check the continuity between subsequent BRP batches. Taking into account the stability data, the results of this collaborative study and on the basis of the central statistical analysis performed at the European Directorate for the Quality of Medicines & HealthCare (EDQM), the 2 candidate batches were officially adopted by the Commission of the European Pharmacopoeia as Heparin low-molecular-mass for assay BRP batches 9 and 10 with assigned anti-Xa activities of 102 and 100 IU/vial and anti-IIa activities of 34 and 33 IU/vial respectively. PMID:27507705

  14. Rapid Diagnosis of Mycobacterial Infections and Quantitation of Mycobacterium tuberculosis Load by Two Real-Time Calibrated PCR Assays

    Science.gov (United States)

    Broccolo, Francesco; Scarpellini, Paolo; Locatelli, Giuseppe; Zingale, Anna; Brambilla, Anna M.; Cichero, Paola; Sechi, Leonardo A.; Lazzarin, Adriano; Lusso, Paolo; Malnati, Mauro S.

    2003-01-01

    Sensitive and specific techniques to detect and identify Mycobacterium tuberculosis directly in clinical specimens are important for the diagnosis and management of patients with tuberculosis (TB). We developed two real-time PCR assays, based on the IS6110 multicopy element and on the senX3-regX3 intergenic region, which provide a rapid method for the diagnosis of mycobacterial infections. The sensitivity and specificity of both assays were established by using purified DNA from 71 clinical isolates and 121 clinical samples collected from 83 patients, 20 of whom were affected by TB. Both assays are accurate, sensitive, and specific, showing a complementary pattern of Mycobacterium recognition: broader for the IS6110-based assay and restricted to the M. tuberculosis complex for the senX3-regX3-based assay. Moreover, the addition of a synthetic DNA calibrator prior to DNA extraction allowed us to measure the efficiency of DNA recovery and to control for the presence of PCR inhibitors. The mycobacterial burden of the clinical samples, as assessed by direct microscopy, correlates with the M. tuberculosis DNA load measured by the senX3-regX3-based assay. In addition, reduced levels of M. tuberculosis DNA load are present in those patients subjected to successful therapy, suggesting a potential use of this assay for monitoring treatment efficacy. Therefore, these assays represent a fully controlled high-throughput system for the evaluation of mycobacterial burden in clinical specimens. PMID:14532183

  15. The Role of Mathematical Methods in Efficiency Calibration and Uncertainty Estimation in Gamma Based Non-Destructive Assay - 12311

    International Nuclear Information System (INIS)

    Mathematical methods are being increasingly employed in the efficiency calibration of gamma based systems for non-destructive assay (NDA) of radioactive waste and for the estimation of the Total Measurement Uncertainty (TMU). Recently, ASTM (American Society for Testing and Materials) released a standard guide for use of modeling passive gamma measurements. This is a testimony to the common use and increasing acceptance of mathematical techniques in the calibration and characterization of NDA systems. Mathematical methods offer flexibility and cost savings in terms of rapidly incorporating calibrations for multiple container types, geometries, and matrix types in a new waste assay system or a system that may already be operational. Mathematical methods are also useful in modeling heterogeneous matrices and non-uniform activity distributions. In compliance with good practice, if a computational method is used in waste assay (or in any other radiological application), it must be validated or benchmarked using representative measurements. In this paper, applications involving mathematical methods in gamma based NDA systems are discussed with several examples. The application examples are from NDA systems that were recently calibrated and performance tested. Measurement based verification results are presented. Mathematical methods play an important role in the efficiency calibration of gamma based NDA systems. This is especially true when the measurement program involves a wide variety of complex item geometries and matrix combinations for which the development of physical standards may be impractical. Mathematical methods offer a cost effective means to perform TMU campaigns. Good practice demands that all mathematical estimates be benchmarked and validated using representative sets of measurements. (authors)

  16. A universally calibrated microplate ferric reducing antioxidant power (FRAP) assay for foods and applications to Manuka honey.

    Science.gov (United States)

    Bolanos de la Torre, Amparo Angelica S; Henderson, Terence; Nigam, Poonam Singh; Owusu-Apenten, Richard K

    2015-05-01

    The ferric reducing antioxidant power (FRAP) assay was recently adapted to a microplate format. However, microplate-based FRAP (mFRAP) assays are affected by sample volume and composition. This work describes a calibration process for mFRAP assays which yields data free of volume effects. From the results, the molar absorptivity (ε) for the mFRAP assay was 141,698 M(-1) cm(-1) for gallic acid, 49,328 M(-1) cm(-1) for ascorbic acid, and 21,606 M(-1) cm(-1) for ammonium ferrous sulphate. The significance of ε (M(-1) cm(-1)) is discussed in relation to mFRAP assay sensitivity, minimum detectable concentration, and the dimensionless FRAP-value. Gallic acid showed 6.6 mol of Fe(2+) equivalents compared to 2.3 mol of Fe(+2) equivalents for ascorbic acid. Application of the mFRAP assay to Manuka honey samples (rated 5+, 10+, 15+, and 18+ Unique Manuka Factor; UMF) showed that FRAP values (0.54-0.76 mmol Fe(2+) per 100g honey) were strongly correlated with UMF ratings (R(2)=0.977) and total phenols content (R(2) = 0.982)whilst the UMF rating was correlated with the total phenols (R(2) = 0.999). In conclusion, mFRAP assay results were successfully standardised to yield data corresponding to 1-cm spectrophotometer which is useful for quality assurance purposes. The antioxidant capacity of Manuka honey was found to be directly related to the UMF rating. PMID:25529660

  17. Plasmid-Based Materials as Multiplex Quality Controls and Calibrators for Clinical Next-Generation Sequencing Assays.

    Science.gov (United States)

    Sims, David J; Harrington, Robin D; Polley, Eric C; Forbes, Thomas D; Mehaffey, Michele G; McGregor, Paul M; Camalier, Corinne E; Harper, Kneshay N; Bouk, Courtney H; Das, Biswajit; Conley, Barbara A; Doroshow, James H; Williams, P Mickey; Lih, Chih-Jian

    2016-05-01

    Although next-generation sequencing technologies have been widely adapted for clinical diagnostic applications, an urgent need exists for multianalyte calibrator materials and controls to evaluate the performance of these assays. Control materials will also play a major role in the assessment, development, and selection of appropriate alignment and variant calling pipelines. We report an approach to provide effective multianalyte controls for next-generation sequencing assays, referred to as the control plasmid spiked-in genome (CPSG). Control plasmids that contain approximately 1000 bases of human genomic sequence with a specific mutation of interest positioned near the middle of the insert and a nearby 6-bp molecular barcode were synthesized, linearized, quantitated, and spiked into genomic DNA derived from formalin-fixed, paraffin-embedded-prepared hapmap cell lines at defined copy number ratios. Serial titration experiments demonstrated the CPSGs performed with similar efficiency of variant detection as formalin-fixed, paraffin-embedded cell line genomic DNA. Repetitive analyses of one lot of CPSGs 90 times during 18 months revealed that the reagents were stable with consistent detection of each of the plasmids at similar variant allele frequencies. CPSGs are designed to work across most next-generation sequencing methods, platforms, and data analysis pipelines. CPSGs are robust controls and can be used to evaluate the performance of different next-generation sequencing diagnostic assays, assess data analysis pipelines, and ensure robust assay performance metrics. PMID:27105923

  18. A High-resolution Typing Assay for Uropathogenic Escherichia coli Based on Fimbrial Diversity.

    Science.gov (United States)

    Ren, Yi; Palusiak, Agata; Wang, Wei; Wang, Yi; Li, Xiao; Wei, Huiting; Kong, Qingke; Rozalski, Antoni; Yao, Zhi; Wang, Quan

    2016-01-01

    Urinary tract infections (UTIs) are one of the most common bacterial infections in humans, causing cystitis, pyelonephritis, and renal failure. Uropathogenic Escherichia coli (UPEC) is the leading cause of UTIs. Accurate and rapid discrimination of UPEC lineages is useful for epidemiological surveillance. Fimbriae are necessary for the adherence of UPEC strains to host uroepithelia, and seem to be abundant and diverse in UPEC strains. By analyzing all the possible fimbrial operons in UPEC strains, we found that closely related strains had similar types of chaperone-usher fimbriae, and the diversity of fimbrial genes was higher than that of multilocus sequence typing (MLST) genes. A typing assay based on the polymorphism of four gene sequences (three fimbrial genes and one housekeeping gene) and the diversity of fimbriae present was developed. By comparison with the MLST, whole-genome sequence (WGS) and fumC/fimH typing methods, this was shown to be accurate and have high resolution, and it was also relatively inexpensive and easy to perform. The assay can supply more discriminatory information for UPEC lineages, and have the potential to be applied in epidemiological surveillance of UPEC isolates.

  19. Extracellular Enzyme Activity assay as indicator of soil microbial functional diversity and activity

    DEFF Research Database (Denmark)

    Hendriksen, Niels Bohse; Winding, Anne

    2012-01-01

    Extracellular Enzyme Activity assay as indicator of soil microbial functional diversity and activity Niels Bohse Hendriksen, Anne Winding. Department of Environmental Science, Aarhus University, 4000 Roskilde, Denmark Soil enzymes originate from a variety of organisms, notably fungi and bacteria...... and especially hydrolytic extracellular enzymes are of pivotal importance for decomposition of organic substrates and biogeochemical cycling. Their activity reflects the functional diversity and activity of the microorganisms involved in decomposition processes which are essential processes for soil functioning...... and soil ecosystem services. The soil enzyme activity has been measured by the use of fluorogenic model substrates e.g. methylumbelliferyl (MUF) substrates for a number of enzymes involved in the degradation of polysaccharides as cellulose, hemicellulose and chitin, while degradation of proteins has been...

  20. Extracellular enzyme activity assay as indicator of soil microbial functional diversity and activity

    DEFF Research Database (Denmark)

    Hendriksen, Niels Bohse; Winding, Anne

    2012-01-01

    Extracellular enzyme activity assay as indicator of soil microbial functional diversity and activity Niels Bohse Hendriksen, Anne Winding. Department of Environmental Science, Aarhus University, 4000 Roskilde, Denmark Soils provide numerous essential ecosystem services such as carbon cycling...... of soil microbial functions is still needed. In soil, enzymes originate from a variety of organisms, notably fungi and bacteria and especially hydrolytic extracellular enzymes are of pivotal importance for decomposition of organic substrates and biogeochemical cycling. Their activity will reflect...... the functional diversity and activity of the microorganisms involved in decomposition processes. Their activity has been measured by the use of fluorogenic model substrates e.g. methylumbelliferyl (MUF) substrates for a number of enzymes involved in the degradation of polysacharides as cellulose, hemicellulose...

  1. Evaluation of a Heparin-Calibrated Antifactor Xa Assay for Measuring the Anticoagulant Effect of Oral Direct Xa Inhibitors.

    Science.gov (United States)

    Beyer, Jacob; Trujillo, Toby; Fisher, Sheila; Ko, Ann; Lind, Stuart E; Kiser, Tyree H

    2016-07-01

    The introduction of oral direct anti-Xa anticoagulants apixaban and rivaroxaban has significantly impacted the treatment and prevention of thromboembolic disease. Clinical scenarios exist in which a quantitative assessment for degree of anticoagulation due to these agents would aid management. The purpose of this work was to evaluate the chromogenic antifactor Xa assay calibrated with heparin standards at our institution for assessment of intensity of anticoagulation with rivaroxaban or apixaban in addition to its current use for unfractionated heparin or low-molecular-weight heparin. We also aimed to propose expected steady state peak and trough antifactor Xa activities for these agents based upon dosing regimens approved for nonvalvular atrial fibrillation. Antifactor Xa activity correlated very strongly with apixaban and rivaroxaban concentration in both spiked samples and treated patient plasma samples (r (2) = .99, P < .001). This correlation was observed over a broad range (20-500 ng/mL) of drug concentrations, as sample dilution with pooled normal plasma significantly extended the range of quantitative assessment. Based on drug concentrations previously published in pharmacokinetic studies, the expected steady state peak and trough antifactor Xa activity ranges for apixaban are 1.80 to 2.20 IU/mL and 0.70 to 1.10 IU/mL, respectively. For rivaroxaban, these ranges are 3.80 to 6.20 IU/mL and 0.60 to 1.00 IU/mL, respectively. In conclusion, our findings demonstrate that heparin-calibrated antifactor Xa activity correlates strongly with apixaban and rivaroxaban concentration. The dilution of samples allowed for this correlation to be extended over the majority of on-therapy drug concentrations. PMID:26842561

  2. Impact of Mutation Type and Amplicon Characteristics on Genetic Diversity Measures Generated Using a High-Resolution Melting Diversity Assay

    OpenAIRE

    Cousins, Matthew M.; Donnell, Deborah; Eshleman, Susan H.

    2013-01-01

    We adapted high-resolution melting (HRM) technology to measure genetic diversity without sequencing. Diversity is measured as a single numeric HRM score. Herein, we determined the impact of mutation types and amplicon characteristics on HRM diversity scores. Plasmids were generated with single-base changes, insertions, and deletions. Different primer sets were used to vary the position of mutations within amplicons. Plasmids and plasmid mixtures were analyzed to determine the impact of mutati...

  3. Genetic diversity in Silene sennenii Pau (Caryophyllaceae assayed through DNA-based techniques

    Directory of Open Access Journals (Sweden)

    Blanché, C.

    2012-12-01

    Full Text Available Silene sennenii is a narrow endemic species from the NE of the Iberian Peninsula. It is considered as EN (“Endangered” according to the IUCN criteria and is under legal protection in Catalonia. In the present work we report an assay using three different approaches for surveying the genetic diversity in this rare, endangered campion: analysis of chloroplast haplotypes, AFLPs and transferability of microsatellites previously developed for Silene latifolia. None of the nine chloroplast regions sequenced showed any variability. Five out of the 21 microsatellite loci tested were polymorphic although more loci are required in order to perform a robust population genetics study. Regarding the AFLP analysis, five out of the 26 pairs of primers tested exhibited moderate levels of variability and therefore they could be useful for further investigating the genetic structure of S. sennenii. Although preliminary, our results based on three different genetic markers are in agreement with the low values of genetic variation already reported for this species employing allozymes.Silene sennenii es una especie endémica, circunscrita a un área extremadamente reducida al NE de la Península Ibérica. Está catalogada como EN («En Peligro» según criterios UICN y se encuentra legalmente protegida en Cataluña. En el presente trabajo se expone el ensayo de tres aproximaciones diferentes al estudio de su diversidad genética: análisis de haplotipos cloroplásticos, AFLPs y transferibilidad de microsatélites diseñados previamente para Silene latifolia. Ninguna de las nueve regiones cloroplásticas secuenciadas ha presentado variabilidad. Se han obtenido cinco loci microsatélites polimórficos de los 21 ensayados, cantidad insuficiente para llevar a cabo un estudio robusto sobre genética poblacional. En cuanto a AFLPs, cinco combinaciones de cebadores de las 26 probadas han mostrado niveles moderados de variabilidad siendo así útiles para posteriores

  4. Improvement of phase diversity algorithm for non-common path calibration in extreme AO context

    Science.gov (United States)

    Robert, Clélia; Fusco, Thierry; Sauvage, Jean-François; Mugnier, Laurent

    2008-07-01

    Exoplanet direct imaging with a ground-based telescope needs a very high performance adaptive optics (AO) system, so-called eXtreme AO (XAO), a coronagraph device, and a smart imaging process. One limitation of AO system in operation remains the Non Common Path Aberrations (NCPA). To achieve the ultimate XAO performance, these aberrations have to be measured with a dedicated wavefront sensor placed in the imaging camera focal plane, and then pre-compensated using the AO closed loop process. In any events, the pre-compensation should minimize the aberrations at the coronagraph focal plane mask. An efficient way for the NCPA measurement is the phase diversity technique. A pixel-wise approach is well-suited to estimate NCPA on large pupils and subsequent projection on the deformable mirror with Cartesian geometry. However it calls for a careful regularization for optimal results. The weight of the regularization is written in close-form for un-supervised tuning. The accuracy of NCPA pre-compensation is below 8 nm for a wide range of conditions. Point-by-point phase estimation improves the accuracy of the Phase Diversity method. The algorithm is validated in simulation and experimentally. It will be implemented in SAXO, the XAO system of the second generation VLT instrument: SPHERE.

  5. Determination of folate concentrations in diverse potato germplasm using a trienzyme extraction and microbiological assay

    Science.gov (United States)

    We determined total folate concentrations of potato tubers from 67 cultivars, advanced breeding lines, or wild species. Folates were extracted by a tri-enzyme treatment and analyzed by using a Lactobacillus rhamnosus microbiological assay. Folate concentrations varied from 521 ± 96 to 1373 ± 230 ng/...

  6. Electroluminescent TCC, C3dg and fB/Bb epitope assays for profiling complement cascade activation in vitro using an activated complement serum calibration standard.

    Science.gov (United States)

    van Vuuren, B Jansen; Bergseth, G; Mollnes, T E; Shaw, A M

    2014-01-15

    Electroluminescent assays for epitopes on the complement components C3dg, terminal complement complex (TCC) and factor B/Bb (fB/Bb) have been developed with capture and detection antibodies to produce detection limits C3dg=91±9ng/mL, TCC=3±0.1ng/mL and fB=55.7±0.1ng/mL. The assay performance was assessed against a series of zymosan and heat aggregated IgG (HAIgG) in vitro activations of complement using a calibrated activated complement serum (ACS) as calibration standard. The ACS standard was stable within 20% accuracy over a 6-month period with freeze-thaw cycles as required. Differential activation of the complement cascade was observed for TCC showing a pseudo-first order formation half-life of 3.5h after activation with zymosan. The C3dg activation fragment indicates a 10% total activation for both activation agents. The kinetic-epitope analysis for fB indicates that the capture epitope is on the fB/Bb protein fragment which can then become covered by the formation of C3bBb or C3bBbP complexes during the time course of the cascade.

  7. Calibration and validation of the 14C-labelled polyethylene glycol-binding assay for tannins in tropical browse

    International Nuclear Information System (INIS)

    This study evaluates the radiolabelled polyethylene glycol (PEG)-binding procedure [Silanikove, N., Shinder, D., Gilboa, N., Eyal, M., Nitsan, Z., 1996. Polyethylene glycol-binding to plant samples as an assay for the biological effects of tannins: predicting the negative effects of tannins in Mediterranean browse on rumen degradation. J. Agric. Food Chem. 44, 3230-3234] for tannin analysis, using 27 tropical browse plants. In this method, the amount of PEG bound to a plant sample is assumed to be a reflection of its tannin content. The method was modified to exclude the use of non-tanniniferous substrate for estimating non-specific binding (NSB) in tannin-containing substrates. Non-specific binding values varied widely (0.4-2.8 mg PEG/100 mg DM tannin-free substrate) when the tannin-free substrate was changed from wheat straw to either rye grass or maize shoots. We therefore propose a modified radiolabelled PEG-binding method to estimate the level of PEG-binding (PEGb) to tannin-containing foliage without using tannin-free substrate to correct for non-specific binding. In this approach, incremental levels of each tanniniferous substrate were used to generate PEGb values. The resultant linear response was analysed and tannin activity was expressed as the slope of the response curve (PEGbSlope) observed for each substrate. The slope takes into account the non-specific binding in each substrate, thus PEGbSlope does not require correction for NSB using tannin-free samples. This approach improved the correlation between PEGb and the 125I-labelled bovine serum albumin precipitation assay. Relationships between the modified PEG-binding assay and radiolabelled bovine serum albumin assay, in vitro tannin bioassay and colorimetric assays are presented. (author)

  8. Calibration and validation of the {sup 14}C-labelled polyethylene glycol-binding assay for tannins in tropical browse

    Energy Technology Data Exchange (ETDEWEB)

    Mlambo, V. [Animal Production Unit, FAO/IAEA Agriculture and Biotechnology Laboratory, Seibersdorf (Austria)]. E-mail: vmlambo@agric.uniswa.sz; Makkar, H.P.S. [Animal Production and Health Section, Joint FAO/IAEA Division of Nuclear Techniques in Agriculture and Food, International Atomic Energy Agency, Vienna (Austria)

    2005-08-19

    This study evaluates the radiolabelled polyethylene glycol (PEG)-binding procedure [Silanikove, N., Shinder, D., Gilboa, N., Eyal, M., Nitsan, Z., 1996. Polyethylene glycol-binding to plant samples as an assay for the biological effects of tannins: predicting the negative effects of tannins in Mediterranean browse on rumen degradation. J. Agric. Food Chem. 44, 3230-3234] for tannin analysis, using 27 tropical browse plants. In this method, the amount of PEG bound to a plant sample is assumed to be a reflection of its tannin content. The method was modified to exclude the use of non-tanniniferous substrate for estimating non-specific binding (NSB) in tannin-containing substrates. Non-specific binding values varied widely (0.4-2.8 mg PEG/100 mg DM tannin-free substrate) when the tannin-free substrate was changed from wheat straw to either rye grass or maize shoots. We therefore propose a modified radiolabelled PEG-binding method to estimate the level of PEG-binding (PEGb) to tannin-containing foliage without using tannin-free substrate to correct for non-specific binding. In this approach, incremental levels of each tanniniferous substrate were used to generate PEGb values. The resultant linear response was analysed and tannin activity was expressed as the slope of the response curve (PEGbSlope) observed for each substrate. The slope takes into account the non-specific binding in each substrate, thus PEGbSlope does not require correction for NSB using tannin-free samples. This approach improved the correlation between PEGb and the {sup 125}I-labelled bovine serum albumin precipitation assay. Relationships between the modified PEG-binding assay and radiolabelled bovine serum albumin assay, in vitro tannin bioassay and colorimetric assays are presented. (author)

  9. A multilocus assay reveals high nucleotide diversity and limited differentiation among Scandinavian willow grouse (Lagopus lagopus

    Directory of Open Access Journals (Sweden)

    Quintela Maria

    2008-12-01

    Full Text Available Abstract Background There is so far very little data on autosomal nucleotide diversity in birds, except for data from the domesticated chicken and some passerines species. Estimates of nucleotide diversity reported so far in birds have been high (~10-3 and a likely explanation for this is the generally higher effective population sizes compared to mammals. In this study, the level of nucleotide diversity has been examined in the willow grouse, a non-domesticated bird species from the order Galliformes, which also holds the chicken. The willow grouse (Lagopus lagopus has an almost circumpolar distribution but is absent from Greenland and the north Atlantic islands. It primarily inhabits tundra, forest edge habitats and sub-alpine vegetation. Willow grouse are hunted throughout its range, and regionally it is a game bird of great cultural and economical importance. Results We sequenced 18 autosomal protein coding loci from approximately 15–18 individuals per population. We found a total of 127 SNP's, which corresponds to 1 SNP every 51 bp. 26 SNP's were amino acid replacement substitutions. Total nucleotide diversity (πt was between 1.30 × 10-4 and 7.66 × 10-3 (average πt = 2.72 × 10-3 ± 2.06 × 10-3 and silent nucleotide diversity varied between 4.20 × 10-4and 2.76 × 10-2 (average πS = 9.22 × 10-3 ± 7.43 × 10-4. The synonymous diversity is approximately 20 times higher than in humans and two times higher than in chicken. Non-synonymous diversity was on average 18 times lower than the synonymous diversity and varied between 0 and 4.90 × 10-3 (average πa = 5.08 × 10-4 ± 7.43 × 103, which suggest that purifying selection is strong in these genes. FST values based on synonymous SNP's varied between -5.60 × 10-4 and 0.20 among loci and revealed low levels of differentiation among the four localities, with an overall value of FST = 0.03 (95% CI: 0.006 – 0.057 over 60 unlinked loci. Non-synonymous SNP's gave similar results. Low

  10. Sequence diversity within the HA-1 gene as detected by melting temperature assay without oligonucleotide probes

    Directory of Open Access Journals (Sweden)

    Mattiuz Pier

    2005-10-01

    Full Text Available Abstract Background The minor histocompatibility antigens (mHags are self-peptides derived from common cellular proteins and presented by MHC class I and II molecules. Disparities in mHags are a potential risk for the development of graft-versus-host disease (GvHD in the recipients of bone marrow from HLA-identical donors. Two alleles have been identified in the mHag HA-1. The correlation between mismatches of the mHag HA-1 and GvHD has been suggested and methods to facilitate large-scale testing were afterwards developed. Methods We used sequence specific primer (SSP PCR and direct sequencing to detect HA-1 gene polymorphisms in a sample of 131 unrelated Italian subjects. We then set up a novel melting temperature (Tm assay that may help identification of HA-1 alleles without oligonucleotide probes. Results We report the frequencies of HA-1 alleles in the Italian population and the presence of an intronic 5 base-pair deletion associated with the immunogeneic allele HA-1H. We also detected novel variable sites with respect to the consensus sequence of HA-1 locus. Even though recombination/gene conversion events are documented, there is considerable linkage disequilibrium in the data. The gametic associations between HA-1R/H alleles and the intronic 5-bp ins/del polymorphism prompted us to try the Tm analysis with SYBR® Green I. We show that the addition of dimethylsulfoxide (DMSO during the assay yields distinct patterns when amplicons from HA-1H homozygotes, HA-1R homozygotes, and heterozygotes are analysed. Conclusion The possibility to use SYBR® Green I to detect Tm differences between allelic variants is attractive but requires great caution. We succeeded in allele discrimination of the HA-1 locus using a relatively short (101 bp amplicon, only in the presence of DMSO. We believe that, at least in certain assets, Tm assays may benefit by the addition of DMSO or other agents affecting DNA strand conformation and stability.

  11. Role of Assay Type in Determining Free 25-Hydroxyvitamin D Levels in Diverse Populations

    Energy Technology Data Exchange (ETDEWEB)

    Nielson, Carrie; Jones, Kerry; Chun, Rene F.; Jacobs, Jon M.; Wang, Ying; Hewison, M.; Adams, John S.; Swanson, Christine; Lee, Christine; Vanderschueren, Dirk; Prentice, Ann; Smith, Richard D.; Shi, Tujin; Gao, Yuqian; Zmuda, Joseph M.; Lapidus, Jodi; Cauley, Jane A.; Bouillon, Roger; Shoenmakers, Inez; Orwoll, Eric S.

    2016-04-28

    Serum 25-hydroxyvitamin D (25OHD) is the most frequently used marker of vitamin D status. Low 25OHD is associated with bone loss, fractures {Cauley, 2010 #1516;Ensrud, 2009 #1517}, and other adverse health outcomes {Theodoratou, 2014 #1518}. Most extra renal tissues only have access to free 25OHD, and free 25OHD concentration is thus a plausible biomarker of 25OHD availability and function {Johnsen, 2014 #1443;Chun, 2014 #1343}. Stronger associations with free or bioavailable 25OHD than with total 25OHD were reported for serum calcium, parathyroid hormone (PTH) {Bhan, 2012 #1124} and bone mineral density (BMD) {Powe, 2011 #1129}. These findings have led to the suggestion that free or bioavailable 25OHD may provide a more clinically relevant measure of tissue 25OHD availability and vitamin D status {Powe, 2013 #1369; Holick NEJM 2013 editorial}. The US Preventive Services Task Force (LeFevre 2015) recently pointed to the gap in research regarding bioavailable and free 25OHD and noted the possibility that these may be better markers of tissue 25OHD availability. Free 25OHD is conventionally calculated from the concentrations of total 25OHD, vitamin D binding protein (DBP) and albumin, with or without a factor accounting for DBP genotype-specific binding affinities {Bouillon, 1981 #1207;Chun, 2012 #1143; Powe, 2011 #1129;Johnsen, 2014 #1443}. DBP—or Group specific component (GC) {Hirschfeld, 1959 #1468}—polymorphisms (rs4588 and rs7041) give rise to three major polymorphic isoforms of DBP, GC-1F, GC-1S and GC-2, the frequencies of which differ globally, with GC-1F alleles more common in populations of African descent {Kambou, 1986 #1122}. Although DBP is a primary component of free and bioavailable 25OHD calculations, substantial variation in DBP between assays has been noted (Powe suggest to cite here previous publications as in previous version of this Ms). Using DBP measured by a monoclonal antibody Powe et al. {Powe, 2013 #1369} concluded that calculated free

  12. New recombinant cyclohexylamine oxidase variants for deracemization of secondary amines by orthogonally assaying designed mutants with structurally diverse substrates

    Science.gov (United States)

    Li, Guangyue; Yao, Peiyuan; Cong, Peiqian; Ren, Jie; Wang, Lei; Feng, Jinhui; Lau, Peter C. K.; Wu, Qiaqing; Zhu, Dunming

    2016-05-01

    To further expand the substrate range of the cyclohexylamine oxidase (CHAO) from Brevibacterium oxydans, a library of diverse mutants was created and assayed toward a group of structurally diverse substrates. Among them, mutants T198A and M226A exhibited enhanced activity relative to wt CHAO for most (S)-enantiomers of primary amines and some secondary amines. While mutants T198I, L199I, L199F, M226I and M226T were more active than wt CHAO toward the primary amines, mutants T198F, L199T, Y321A, Y321T, Y321I and Y321F enhanced the enzyme activity toward the secondary amines. In particular, mutant Y321I displayed an enhanced catalytic efficiency toward 1-(4-methoxybenzyl)-1, 2, 3, 4, 5, 6, 7, 8-octahydroisoquinoline (13). Whereas a double mutant, Y321I/M226T, acted on (S)-N-(prop-2-yn-1-yl)-2, 3-dihydro-1H-inden-1-amine [(S)-8]. Since (R)-8 is an irreversible inhibitor of monoamine oxidase and (S)-13 is an intermediate of dextromethorphan, a cough suppressant drug, deracemizations of 8 and 13 were carried out with crude enzyme extracts of the respective mutants. This resulted in 51% and 78% isolated yields of (R)-8 and (S)-13, respectively, each with high enantiomeric excess (93% and 99% ee). The results demonstrated the application potential of the evolved CHAO mutants in drug synthesis requiring chiral secondary amines.

  13. Editor's Highlight: Analysis of the Effects of Cell Stress and Cytotoxicity on In Vitro Assay Activity Across a Diverse Chemical and Assay Space.

    Science.gov (United States)

    Judson, Richard; Houck, Keith; Martin, Matt; Richard, Ann M; Knudsen, Thomas B; Shah, Imran; Little, Stephen; Wambaugh, John; Woodrow Setzer, R; Kothya, Parth; Phuong, Jimmy; Filer, Dayne; Smith, Doris; Reif, David; Rotroff, Daniel; Kleinstreuer, Nicole; Sipes, Nisha; Xia, Menghang; Huang, Ruili; Crofton, Kevin; Thomas, Russell S

    2016-08-01

    Chemical toxicity can arise from disruption of specific biomolecular functions or through more generalized cell stress and cytotoxicity-mediated processes. Here, responses of 1060 chemicals including pharmaceuticals, natural products, pesticidals, consumer, and industrial chemicals across a battery of 815 in vitro assay endpoints from 7 high-throughput assay technology platforms were analyzed in order to distinguish between these types of activities. Both cell-based and cell-free assays showed a rapid increase in the frequency of responses at concentrations where cell stress/cytotoxicity responses were observed in cell-based assays. Chemicals that were positive on at least 2 viability/cytotoxicity assays within the concentration range tested (typically up to 100 μM) activated a median of 12% of assay endpoints whereas those that were not cytotoxic in this concentration range activated 1.3% of the assays endpoints. The results suggest that activity can be broadly divided into: (1) specific biomolecular interactions against one or more targets (eg, receptors or enzymes) at concentrations below which overt cytotoxicity-associated activity is observed; and (2) activity associated with cell stress or cytotoxicity, which may result from triggering specific cell stress pathways, chemical reactivity, physico-chemical disruption of proteins or membranes, or broad low-affinity non-covalent interactions. Chemicals showing a greater number of specific biomolecular interactions are generally designed to be bioactive (pharmaceuticals or pesticidal active ingredients), whereas intentional food-use chemicals tended to show the fewest specific interactions. The analyses presented here provide context for use of these data in ongoing studies to predict in vivo toxicity from chemicals lacking extensive hazard assessment. PMID:27208079

  14. Editor's Highlight: Analysis of the Effects of Cell Stress and Cytotoxicity on In Vitro Assay Activity Across a Diverse Chemical and Assay Space.

    Science.gov (United States)

    Judson, Richard; Houck, Keith; Martin, Matt; Richard, Ann M; Knudsen, Thomas B; Shah, Imran; Little, Stephen; Wambaugh, John; Woodrow Setzer, R; Kothya, Parth; Phuong, Jimmy; Filer, Dayne; Smith, Doris; Reif, David; Rotroff, Daniel; Kleinstreuer, Nicole; Sipes, Nisha; Xia, Menghang; Huang, Ruili; Crofton, Kevin; Thomas, Russell S

    2016-08-01

    Chemical toxicity can arise from disruption of specific biomolecular functions or through more generalized cell stress and cytotoxicity-mediated processes. Here, responses of 1060 chemicals including pharmaceuticals, natural products, pesticidals, consumer, and industrial chemicals across a battery of 815 in vitro assay endpoints from 7 high-throughput assay technology platforms were analyzed in order to distinguish between these types of activities. Both cell-based and cell-free assays showed a rapid increase in the frequency of responses at concentrations where cell stress/cytotoxicity responses were observed in cell-based assays. Chemicals that were positive on at least 2 viability/cytotoxicity assays within the concentration range tested (typically up to 100 μM) activated a median of 12% of assay endpoints whereas those that were not cytotoxic in this concentration range activated 1.3% of the assays endpoints. The results suggest that activity can be broadly divided into: (1) specific biomolecular interactions against one or more targets (eg, receptors or enzymes) at concentrations below which overt cytotoxicity-associated activity is observed; and (2) activity associated with cell stress or cytotoxicity, which may result from triggering specific cell stress pathways, chemical reactivity, physico-chemical disruption of proteins or membranes, or broad low-affinity non-covalent interactions. Chemicals showing a greater number of specific biomolecular interactions are generally designed to be bioactive (pharmaceuticals or pesticidal active ingredients), whereas intentional food-use chemicals tended to show the fewest specific interactions. The analyses presented here provide context for use of these data in ongoing studies to predict in vivo toxicity from chemicals lacking extensive hazard assessment.

  15. Type-specific PCR assays for Babesia bovis msa-1 genotypes in Asia: Revisiting the genetic diversity in Sri Lanka, Mongolia, and Vietnam.

    Science.gov (United States)

    Liyanagunawardena, Nilukshi; Sivakumar, Thillaiampalam; Kothalawala, Hemal; Silva, Seekkuge Susil Priyantha; Battsetseg, Badgar; Lan, Dinh Thi Bich; Inoue, Noboru; Igarashi, Ikuo; Yokoyama, Naoaki

    2016-01-01

    Babesia bovis is the most virulent Babesia organism, resulting in a high mortality rate in cattle. The genetic diversity of B. bovis merozoite surface antigens (MSAs), such as MSA-1, MSA-2b, and MSA-2c, might be linked to altered immune profiles in the host animals. The present study aimed to develop type-specific PCR assays for Asian msa-1 genotypes, thereby re-analyzing the genetic diversity of msa-1 in Sri Lanka, Mongolia, and Vietnam. Specific primers were designed for nine Asian msa-1 genotypes, which had been detected based on the phylogeny constructed using msa-1 gene sequences retrieved from the GenBank database. Specificity of the type-specific PCR assays was confirmed using plasmids containing the inserts of msa-1 gene fragments that represent Asian genotypes. Furthermore, no amplicons were observed by these PCR assays when DNA samples of Babesia bigemina, Babesia ovata, Theileria annulata, Theileria orientalis, Trypanosoma evansi, Trypanosoma theileri, Anaplasma marginale, and Anaplasma bovis, and non-infected bovine blood were analyzed. In total, 109 B. bovis-positive blood DNA samples sourced from Sri Lanka (44 cattle), Mongolia (26 cattle), and Vietnam (23 cattle and 16 water buffaloes) were then screened by the type-specific PCR assays. The sequences derived from all of the PCR amplicons were phylogenetically analyzed. Out of 109 DNA samples, 23 (20 from cattle and 3 from water buffaloes) were positive for at least one genotype. In agreement with previous studies, five and four different genotypes were detected among the DNA samples from Sri Lanka and Vietnam, respectively. In contrast, four genotypes, including three novel genotypes, were detected from Mongolia. Five DNA samples were found to be co-infected with multiple genotypes. The sequences of the PCR amplicons clustered phylogenetically within the corresponding clades. These findings indicated that the type-specific PCR assays described herein are useful for the determination of genotypic

  16. Type-specific PCR assays for Babesia bovis msa-1 genotypes in Asia: Revisiting the genetic diversity in Sri Lanka, Mongolia, and Vietnam.

    Science.gov (United States)

    Liyanagunawardena, Nilukshi; Sivakumar, Thillaiampalam; Kothalawala, Hemal; Silva, Seekkuge Susil Priyantha; Battsetseg, Badgar; Lan, Dinh Thi Bich; Inoue, Noboru; Igarashi, Ikuo; Yokoyama, Naoaki

    2016-01-01

    Babesia bovis is the most virulent Babesia organism, resulting in a high mortality rate in cattle. The genetic diversity of B. bovis merozoite surface antigens (MSAs), such as MSA-1, MSA-2b, and MSA-2c, might be linked to altered immune profiles in the host animals. The present study aimed to develop type-specific PCR assays for Asian msa-1 genotypes, thereby re-analyzing the genetic diversity of msa-1 in Sri Lanka, Mongolia, and Vietnam. Specific primers were designed for nine Asian msa-1 genotypes, which had been detected based on the phylogeny constructed using msa-1 gene sequences retrieved from the GenBank database. Specificity of the type-specific PCR assays was confirmed using plasmids containing the inserts of msa-1 gene fragments that represent Asian genotypes. Furthermore, no amplicons were observed by these PCR assays when DNA samples of Babesia bigemina, Babesia ovata, Theileria annulata, Theileria orientalis, Trypanosoma evansi, Trypanosoma theileri, Anaplasma marginale, and Anaplasma bovis, and non-infected bovine blood were analyzed. In total, 109 B. bovis-positive blood DNA samples sourced from Sri Lanka (44 cattle), Mongolia (26 cattle), and Vietnam (23 cattle and 16 water buffaloes) were then screened by the type-specific PCR assays. The sequences derived from all of the PCR amplicons were phylogenetically analyzed. Out of 109 DNA samples, 23 (20 from cattle and 3 from water buffaloes) were positive for at least one genotype. In agreement with previous studies, five and four different genotypes were detected among the DNA samples from Sri Lanka and Vietnam, respectively. In contrast, four genotypes, including three novel genotypes, were detected from Mongolia. Five DNA samples were found to be co-infected with multiple genotypes. The sequences of the PCR amplicons clustered phylogenetically within the corresponding clades. These findings indicated that the type-specific PCR assays described herein are useful for the determination of genotypic

  17. Calibrating snakehead diversity with DNA barcodes: expanding taxonomic coverage to enable identification of potential and established invasive species.

    Science.gov (United States)

    Serrao, Natasha R; Steinke, Dirk; Hanner, Robert H

    2014-01-01

    Detecting and documenting the occurrence of invasive species outside their native range requires tools to support their identification. This can be challenging for taxa with diverse life stages and/or problematic or unresolved morphological taxonomies. DNA barcoding provides a potent method for identifying invasive species, as it allows for species identification at all life stages, including fragmentary remains. It also provides an efficient interim taxonomic framework for quantifying cryptic genetic diversity by parsing barcode sequences into discontinuous haplogroup clusters (typical of reproductively isolated species) and labelling them with unique alphanumeric identifiers. Snakehead fishes are a diverse group of opportunistic predators endemic to Asia and Africa that may potentially pose significant threats as aquatic invasive species. At least three snakehead species (Channa argus, C. maculata, and C. marulius) are thought to have entered North America through the aquarium and live-food fish markets, and have established populations, yet their origins remain unclear. The objectives of this study were to assemble a library of DNA barcode sequences derived from expert identified reference specimens in order to determine the identity and aid invasion pathway analysis of the non-indigenous species found in North America using DNA barcodes. Sequences were obtained from 121 tissue samples representing 25 species and combined with public records from GenBank for a total of 36 putative species, which then partitioned into 49 discrete haplogroups. Multiple divergent clusters were observed within C. gachua, C. marulius, C. punctata and C. striata suggesting the potential presence of cryptic species diversity within these lineages. Our findings demonstrate that DNA barcoding is a valuable tool for species identification in challenging and under-studied taxonomic groups such as snakeheads, and provides a useful framework for inferring invasion pathway analysis.

  18. Calibrating snakehead diversity with DNA barcodes: expanding taxonomic coverage to enable identification of potential and established invasive species.

    Directory of Open Access Journals (Sweden)

    Natasha R Serrao

    Full Text Available Detecting and documenting the occurrence of invasive species outside their native range requires tools to support their identification. This can be challenging for taxa with diverse life stages and/or problematic or unresolved morphological taxonomies. DNA barcoding provides a potent method for identifying invasive species, as it allows for species identification at all life stages, including fragmentary remains. It also provides an efficient interim taxonomic framework for quantifying cryptic genetic diversity by parsing barcode sequences into discontinuous haplogroup clusters (typical of reproductively isolated species and labelling them with unique alphanumeric identifiers. Snakehead fishes are a diverse group of opportunistic predators endemic to Asia and Africa that may potentially pose significant threats as aquatic invasive species. At least three snakehead species (Channa argus, C. maculata, and C. marulius are thought to have entered North America through the aquarium and live-food fish markets, and have established populations, yet their origins remain unclear. The objectives of this study were to assemble a library of DNA barcode sequences derived from expert identified reference specimens in order to determine the identity and aid invasion pathway analysis of the non-indigenous species found in North America using DNA barcodes. Sequences were obtained from 121 tissue samples representing 25 species and combined with public records from GenBank for a total of 36 putative species, which then partitioned into 49 discrete haplogroups. Multiple divergent clusters were observed within C. gachua, C. marulius, C. punctata and C. striata suggesting the potential presence of cryptic species diversity within these lineages. Our findings demonstrate that DNA barcoding is a valuable tool for species identification in challenging and under-studied taxonomic groups such as snakeheads, and provides a useful framework for inferring invasion pathway

  19. Calibrating snakehead diversity with DNA barcodes: expanding taxonomic coverage to enable identification of potential and established invasive species.

    Science.gov (United States)

    Serrao, Natasha R; Steinke, Dirk; Hanner, Robert H

    2014-01-01

    Detecting and documenting the occurrence of invasive species outside their native range requires tools to support their identification. This can be challenging for taxa with diverse life stages and/or problematic or unresolved morphological taxonomies. DNA barcoding provides a potent method for identifying invasive species, as it allows for species identification at all life stages, including fragmentary remains. It also provides an efficient interim taxonomic framework for quantifying cryptic genetic diversity by parsing barcode sequences into discontinuous haplogroup clusters (typical of reproductively isolated species) and labelling them with unique alphanumeric identifiers. Snakehead fishes are a diverse group of opportunistic predators endemic to Asia and Africa that may potentially pose significant threats as aquatic invasive species. At least three snakehead species (Channa argus, C. maculata, and C. marulius) are thought to have entered North America through the aquarium and live-food fish markets, and have established populations, yet their origins remain unclear. The objectives of this study were to assemble a library of DNA barcode sequences derived from expert identified reference specimens in order to determine the identity and aid invasion pathway analysis of the non-indigenous species found in North America using DNA barcodes. Sequences were obtained from 121 tissue samples representing 25 species and combined with public records from GenBank for a total of 36 putative species, which then partitioned into 49 discrete haplogroups. Multiple divergent clusters were observed within C. gachua, C. marulius, C. punctata and C. striata suggesting the potential presence of cryptic species diversity within these lineages. Our findings demonstrate that DNA barcoding is a valuable tool for species identification in challenging and under-studied taxonomic groups such as snakeheads, and provides a useful framework for inferring invasion pathway analysis. PMID

  20. [Multisite-based approach to assure inter-assay system compatibility among different exclusive-typed immunoassay systems through determining exchanged calibrators].

    Science.gov (United States)

    Yamauchi, Megumi S; Yamane, Nobuhisa; Toshimitsu, Shoji; Sato, Hisatsune; Fujino, Tatsuya

    2010-01-01

    It is well known that most exclusive-typed immunoassay systems are highly precise but are poor in compatibility of their determinations. Thus, it is difficult to compare the determinations among different systems, posing problems when a patient is transferred to different hospitals or when a laboratory intends to change the system currently used. In the study, we tried to approach how to assure inter-immunoassay compatibility among four different systems through determination of the exchanged calibrators. First, determinations of total protein and albumin, and electrophoretic fractionation demonstrated marked differences among calibrators in their protein constituent. Some calibrators were prepared with human sera, but others were with inorganic or non-human albumin-based solution. Regression analysis of calibrators between the indicated concentrations by manufacturers and those actually determined by the different immunoassay systems revealed that; most slopes were closed to 1.0 for alpha-fetoprotein and prostate-specific antigen, but widely dissociated from 0.28 to 4.71 for CA19-9. In evaluation of clinical serum samples, determinations by one immunoassay system were compared with those converted based on a linear regression equation that was obtained by determination of the exchanged calibrators. However, this procedure could not improve compatibility, and positive effects of conversion varied by immunoassay systems combined, and also by test parameters. With these, we concluded that simple conversion of determinations by using the exchanged calibrators and a statistical linear regression could not provide us with the expected compatibility. Thus, standardization of target molecules or probes, and of calibrator constituent were urgent issue to assure inter-immunoassay compatibility. PMID:20169939

  1. Application of the neuroblastoma assay for paralytic shellfish poisons to neurotoxic freshwater cyanobacteria: interlaboratory calibration and comparison with other methods of analysis.

    Science.gov (United States)

    Humpage, Andrew R; Ledreux, Aurélie; Fanok, Stella; Bernard, Cécile; Briand, Jean-François; Eaglesham, Geoff; Papageorgiou, John; Nicholson, Brenton; Steffensen, Dennis

    2007-07-01

    Paralytic shellfish poisons (PSPs) are produced by freshwater cyanobacteria and pose a threat to human and animal drinking-water supplies. The wide range of toxin analogues (and the likelihood that further analogues remain to be discovered) means that chromatographic methods are not always reliable indicators of toxicity. Although the mouse bioassay remains the method of choice in the seafood industry, its use is increasingly being questioned on ethical grounds. The cell-based Neuro-2A neuroblastoma toxicity assay is an alternative bioassay validated for testing shellfish extracts, so it was of interest to determine its applicability with the different suite of toxin analogues produced by cyanobacteria. Cyanobacterial bloom samples from Australia, Brazil, and France were assayed using the neuroblastoma assay, liquid chromatography-tandem mass spectrometry (LC-MS/MS), high-performance liquid chromatography with postcolumn derivatization and fluorescence detection, and the Jellett Rapid Test for PSP. To assess interlaboratory variability, the neuroblastoma assay was set up in laboratories in Paris (France) and Adelaide (Australia). Neuroblastoma and chromatographic methods gave comparable results except in the case of the neurotoxic Brazilian samples: LC-MS/MS did not detect the putative new PSPs contained in these samples. Inter- and intralaboratory variability of the neuroblastoma assay was typical of biological assays but no greater than that found for interassay variability between different chromatographic determinations. The batch of Jellett Rapid Tests for PSP used did not yield quantitative results. Overall, the neuroblastoma assay was useful as a screening assay for determination of toxicity caused by saxitoxin neurotoxins in freshwater cyanobacteria, having the advantage of being sensitive to unidentified toxins that currently cannot be quantified by chromatographic means. PMID:17665694

  2. 32P-adduct assay: Comparative recoveries of structurally diverse DNA adducts in the various enhancement procedures

    International Nuclear Information System (INIS)

    A (32)P-adduct assay for the measurement of low levels (1 adduct per 10(sup 7) nucleotides) of binding of carcinogens to DNA has been reported previously. In this procedure, DNA is enzymatically hydrolyzed to 3'-monophosphates of normal nucleosides and adducts, which are 5'-(32)P-labeled by T4 polynucleotide kinase and (lambda(32)P)ATP. Labeled adducts are resolved by TLC. Enrichment of adducts by extraction in 1-butanol or digestion with nuclease P1 prior to (32)P-labeling, however, increased the sensitivity of detection for many adducts to a level of 1 per 10(sup 9-10) nucleotides, although adduct recovery particularly in the latter assay depended on the chemical nature of adducts. The observation that chemical structure of an adduct may be detrimental in its recovery in the enzyme- and extraction-mediated enrichment procedures may serve as a probe in the structural characterization of adducts of unknown carcinogens

  3. Rover waste assay system

    Energy Technology Data Exchange (ETDEWEB)

    Akers, D.W.; Stoots, C.M.; Kraft, N.C.; Marts, D.J. [Idaho National Engineering Lab., Idaho Falls, ID (United States)

    1997-11-01

    The Rover Waste Assay System (RWAS) is a nondestructive assay system designed for the rapid assay of highly-enriched {sup 235}U contaminated piping, tank sections, and debris from the Rover nuclear rocket fuel processing facility at the Idaho Chemical Processing Plant. A scanning system translates a NaI(Tl) detector/collimator system over the structural components where both relative and calibrated measurements for {sup 137}Cs are made. Uranium-235 concentrations are in operation and is sufficiently automated that most functions are performed by the computer system. These functions include system calibration, problem identification, collimator control, data analysis, and reporting. Calibration of the system was done through a combination of measurements on calibration standards and benchmarked modeling. A description of the system is presented along with the methods and uncertainties associated with the calibration and analysis of the system for components from the Rover facility. 4 refs., 2 figs., 4 tabs.

  4. Rover waste assay system

    International Nuclear Information System (INIS)

    The Rover Waste Assay System (RWAS) is a nondestructive assay system designed for the rapid assay of highly-enriched 235U contaminated piping, tank sections, and debris from the Rover nuclear rocket fuel processing facility at the Idaho Chemical Processing Plant. A scanning system translates a NaI(Tl) detector/collimator system over the structural components where both relative and calibrated measurements for 137Cs are made. Uranium-235 concentrations are in operation and is sufficiently automated that most functions are performed by the computer system. These functions include system calibration, problem identification, collimator control, data analysis, and reporting. Calibration of the system was done through a combination of measurements on calibration standards and benchmarked modeling. A description of the system is presented along with the methods and uncertainties associated with the calibration and analysis of the system for components from the Rover facility. 4 refs., 2 figs., 4 tabs

  5. Interpreting sperm DNA damage in a diverse range of mammalian sperm by means of the two-tailed comet assay

    OpenAIRE

    Cortés-Gutiérrez, Elva I.; López-Fernández, Carmen; Fernández, José Luis; DÁVILA-RODRÍGUEZ, MARTHA I.; Johnston, Stephen D; Gosálvez, Jaime

    2014-01-01

    Key Concepts The two-dimensional Two-Tailed Comet assay (TT-comet) protocol is a valuable technique to differentiate between single-stranded (SSBs) and double-stranded DNA breaks (DSBs) on the same sperm cell. Protein lysis inherent with the TT-comet protocol accounts for differences in sperm protamine composition at a species-specific level to produce reliable visualization of sperm DNA damage. Alkaline treatment may break the sugar–phosphate backbone in abasic sites or at sites wi...

  6. Assay methods for U-238, Th-232, and Pb-210 in lead and calibration of Bi-210 bremsstrahlung emission from lead

    CERN Document Server

    Orrell, John L; Arnquist, Isaac J; Eggemeyer, Tere A; Glasgow, Brian D; Hoppe, Eric W; Keillor, Martin E; Morley, Shannon M; Myers, Allan W; Overman, Cory T; Shaff, Sarah M; Thommasson, Kimbrelle S

    2015-01-01

    Assay methods for measuring 238U, 232Th, and 210Pb concentrations in refined lead are presented. The 238U and 232Th concentrations are determined using isotope dilution inductively coupled plasma mass spectrometry (ID-ICP-MS) after anion exchange column separation of dissolved lead samples. The 210Pb concentration is inferred through {\\alpha}-spectroscopy of a daughter isotope, 210Po, after chemical precipitation separation on dissolved lead samples. Subsequent to the 210Po {\\alpha}-spectroscopy assay, a method for evaluating 210Pb concentrations in solid lead samples was developed via measurement of bremsstrahlung radiation from \\b{eta}-decay of a daughter isotope, 210Bi, by employing a 14-crystal array of high purity germanium (HPGe) detectors. Ten sources of refined lead were assayed. The 238U concentrations were <34 microBq/kg and the 232Th concentrations ranged <0.6-15 microBq/kg, as determined by the ICP-MS assay method. The 210Pb concentrations ranged from ~0.1-75 Bq/kg, as inferred by the 210Po ...

  7. A lack of premature termination codon read-through efficacy of PTC124 (Ataluren in a diverse array of reporter assays.

    Directory of Open Access Journals (Sweden)

    Stuart P McElroy

    Full Text Available The drug molecule PTC124 (Ataluren has been described as a read-through agent, capable of suppressing premature termination codons (PTCs and restoring functional protein production from genes disrupted by nonsense mutations. Following the discovery of PTC124 there was some controversy regarding its mechanism of action with two reports attributing its activity to an off-target effect on the Firefly luciferase (FLuc reporter used in the development of the molecule. Despite questions remaining as to its mechanism of action, development of PTC124 continued into the clinic and it is being actively pursued as a potential nonsense mutation therapy. To thoroughly test the ability of PTC124 to read through nonsense mutations, we conducted a detailed assessment comparing the efficacy of PTC124 with the classical aminoglycoside antibiotic read-through agent geneticin (G418 across a diverse range of in vitro reporter assays. We can confirm the off-target FLuc activity of PTC124 but found that, while G418 exhibits varying activity in every read-through assay, there is no evidence of activity for PTC124.

  8. Calibration of the γ-H2AX DNA double strand break focus assay for internal radiation exposure of blood lymphocytes.

    Science.gov (United States)

    Eberlein, Uta; Peper, Michel; Fernández, Maria; Lassmann, Michael; Scherthan, Harry

    2015-01-01

    DNA double strand break (DSB) formation induced by ionizing radiation exposure is indicated by the DSB biomarkers γ-H2AX and 53BP1. Knowledge about DSB foci formation in-vitro after internal irradiation of whole blood samples with radionuclides in solution will help us to gain detailed insights about dose-response relationships in patients after molecular radiotherapy (MRT). Therefore, we studied the induction of radiation-induced co-localizing γ-H2AX and 53BP1 foci as surrogate markers for DSBs in-vitro, and correlated the obtained foci per cell values with the in-vitro absorbed doses to the blood for the two most frequently used radionuclides in MRT (I-131 and Lu-177). This approach led to an in-vitro calibration curve. Overall, 55 blood samples of three healthy volunteers were analyzed. For each experiment several vials containing a mixture of whole blood and radioactive solutions with different concentrations of isotonic NaCl-diluted radionuclides with known activities were prepared. Leukocytes were recovered by density centrifugation after incubation and constant blending for 1 h at 37°C. After ethanol fixation they were subjected to two-color immunofluorescence staining and the average frequencies of the co-localizing γ-H2AX and 53BP1 foci/nucleus were determined using a fluorescence microscope equipped with a red/green double band pass filter. The exact activity was determined in parallel in each blood sample by calibrated germanium detector measurements. The absorbed dose rates to the blood per nuclear disintegrations occurring in 1 ml of blood were calculated for both isotopes by a Monte Carlo simulation. The measured blood doses in our samples ranged from 6 to 95 mGy. A linear relationship was found between the number of DSB-marking foci/nucleus and the absorbed dose to the blood for both radionuclides studied. There were only minor nuclide-specific intra- and inter-subject deviations.

  9. Calibration of the γ-H2AX DNA double strand break focus assay for internal radiation exposure of blood lymphocytes.

    Directory of Open Access Journals (Sweden)

    Uta Eberlein

    Full Text Available DNA double strand break (DSB formation induced by ionizing radiation exposure is indicated by the DSB biomarkers γ-H2AX and 53BP1. Knowledge about DSB foci formation in-vitro after internal irradiation of whole blood samples with radionuclides in solution will help us to gain detailed insights about dose-response relationships in patients after molecular radiotherapy (MRT. Therefore, we studied the induction of radiation-induced co-localizing γ-H2AX and 53BP1 foci as surrogate markers for DSBs in-vitro, and correlated the obtained foci per cell values with the in-vitro absorbed doses to the blood for the two most frequently used radionuclides in MRT (I-131 and Lu-177. This approach led to an in-vitro calibration curve. Overall, 55 blood samples of three healthy volunteers were analyzed. For each experiment several vials containing a mixture of whole blood and radioactive solutions with different concentrations of isotonic NaCl-diluted radionuclides with known activities were prepared. Leukocytes were recovered by density centrifugation after incubation and constant blending for 1 h at 37°C. After ethanol fixation they were subjected to two-color immunofluorescence staining and the average frequencies of the co-localizing γ-H2AX and 53BP1 foci/nucleus were determined using a fluorescence microscope equipped with a red/green double band pass filter. The exact activity was determined in parallel in each blood sample by calibrated germanium detector measurements. The absorbed dose rates to the blood per nuclear disintegrations occurring in 1 ml of blood were calculated for both isotopes by a Monte Carlo simulation. The measured blood doses in our samples ranged from 6 to 95 mGy. A linear relationship was found between the number of DSB-marking foci/nucleus and the absorbed dose to the blood for both radionuclides studied. There were only minor nuclide-specific intra- and inter-subject deviations.

  10. Calibration of the γ-H2AX DNA double strand break focus assay for internal radiation exposure of blood lymphocytes.

    Science.gov (United States)

    Eberlein, Uta; Peper, Michel; Fernández, Maria; Lassmann, Michael; Scherthan, Harry

    2015-01-01

    DNA double strand break (DSB) formation induced by ionizing radiation exposure is indicated by the DSB biomarkers γ-H2AX and 53BP1. Knowledge about DSB foci formation in-vitro after internal irradiation of whole blood samples with radionuclides in solution will help us to gain detailed insights about dose-response relationships in patients after molecular radiotherapy (MRT). Therefore, we studied the induction of radiation-induced co-localizing γ-H2AX and 53BP1 foci as surrogate markers for DSBs in-vitro, and correlated the obtained foci per cell values with the in-vitro absorbed doses to the blood for the two most frequently used radionuclides in MRT (I-131 and Lu-177). This approach led to an in-vitro calibration curve. Overall, 55 blood samples of three healthy volunteers were analyzed. For each experiment several vials containing a mixture of whole blood and radioactive solutions with different concentrations of isotonic NaCl-diluted radionuclides with known activities were prepared. Leukocytes were recovered by density centrifugation after incubation and constant blending for 1 h at 37°C. After ethanol fixation they were subjected to two-color immunofluorescence staining and the average frequencies of the co-localizing γ-H2AX and 53BP1 foci/nucleus were determined using a fluorescence microscope equipped with a red/green double band pass filter. The exact activity was determined in parallel in each blood sample by calibrated germanium detector measurements. The absorbed dose rates to the blood per nuclear disintegrations occurring in 1 ml of blood were calculated for both isotopes by a Monte Carlo simulation. The measured blood doses in our samples ranged from 6 to 95 mGy. A linear relationship was found between the number of DSB-marking foci/nucleus and the absorbed dose to the blood for both radionuclides studied. There were only minor nuclide-specific intra- and inter-subject deviations. PMID:25853575

  11. Interferon Gamma Release Assay versus Tuberculin Skin Testing among Healthcare Workers of Highly Diverse Origin in a Moderate Tuberculosis Burden Country.

    Science.gov (United States)

    Al Hajoj, Sahal; Varghese, Bright; Datijan, Alria; Shoukri, Mohammed; Alzahrani, Ali; Alkhenizan, Abdallah; AlSaif, Abdulaziz; Althawadi, Sahar; Fernandez, Grace; Alrajhi, Abdulrahman

    2016-01-01

    Health care workers (HCW's) are always at an increased risk of contracting tuberculosis (TB) infection. In Saudi Arabia, Interferon Gamma Release Assay (IGRA) has not been evaluated as a screening tool for latent TB infection (LTBI) among HCW's considering their high demographic diversity. During February 2012 to January 2015 a cross sectional study has been conducted in a tertiary care center with maximum demographically diverse staff population in the capital city-Riyadh. After a short interview and consenting, all the candidates were subjected to tuberculin skin test (TST) and QuantiFERON TB gold In-tube test (QFT). A logistic regression analysis was carried out for establishing the associations between putative risk factors and the diagnostic tests. The candidates were classified according to geographical origin and a detailed analysis was conducted on the impact of their origin towards the results of TST and QFT. Of the 1595 candidates enrolled, 90.6% were BCG vaccinated, female (67.9%) and mainly nurses (53.2%). Candidates with high risk of suspected or confirmed TB patient exposure were 56.1% and 76.5% of them had origin were associated with TST positivity while Occupational TB exposure and geographical origin of the candidates were associated with QFT positivity. A regular follow up on recently TST converted candidates showed no progression to active TB. The putative factors associated with the discordance were origin of the candidate (p value Origin of the candidates has strong association with the results of TST and QFT. The discordant results particularly TST negative and QFT positive needs more detailed analysis. PMID:27148876

  12. Interferon Gamma Release Assay versus Tuberculin Skin Testing among Healthcare Workers of Highly Diverse Origin in a Moderate Tuberculosis Burden Country.

    Science.gov (United States)

    Al Hajoj, Sahal; Varghese, Bright; Datijan, Alria; Shoukri, Mohammed; Alzahrani, Ali; Alkhenizan, Abdallah; AlSaif, Abdulaziz; Althawadi, Sahar; Fernandez, Grace; Alrajhi, Abdulrahman

    2016-01-01

    Health care workers (HCW's) are always at an increased risk of contracting tuberculosis (TB) infection. In Saudi Arabia, Interferon Gamma Release Assay (IGRA) has not been evaluated as a screening tool for latent TB infection (LTBI) among HCW's considering their high demographic diversity. During February 2012 to January 2015 a cross sectional study has been conducted in a tertiary care center with maximum demographically diverse staff population in the capital city-Riyadh. After a short interview and consenting, all the candidates were subjected to tuberculin skin test (TST) and QuantiFERON TB gold In-tube test (QFT). A logistic regression analysis was carried out for establishing the associations between putative risk factors and the diagnostic tests. The candidates were classified according to geographical origin and a detailed analysis was conducted on the impact of their origin towards the results of TST and QFT. Of the 1595 candidates enrolled, 90.6% were BCG vaccinated, female (67.9%) and mainly nurses (53.2%). Candidates with high risk of suspected or confirmed TB patient exposure were 56.1% and 76.5% of them had origin were associated with TST positivity while Occupational TB exposure and geographical origin of the candidates were associated with QFT positivity. A regular follow up on recently TST converted candidates showed no progression to active TB. The putative factors associated with the discordance were origin of the candidate (p value Origin of the candidates has strong association with the results of TST and QFT. The discordant results particularly TST negative and QFT positive needs more detailed analysis.

  13. Characterization of Genetic Diversity of Bacillus anthracis in France by Using High-Resolution Melting Assays and Multilocus Variable-Number Tandem-Repeat Analysis ▿ †

    Science.gov (United States)

    Derzelle, S.; Laroche, S.; Le Flèche, P.; Hauck, Y.; Thierry, S.; Vergnaud, G.; Madani, N.

    2011-01-01

    Using high-resolution melting (HRM) analysis, we developed a cost-effective method to genotype a set of 13 phylogenetically informative single-nucleotide polymorphisms (SNPs) within the genome of Bacillus anthracis. SNP discrimination assays were performed in monoplex or duplex and applied to 100 B. anthracis isolates collected in France from 1953 to 2009 and a few reference strains. HRM provided a reliable and cheap alternative to subtype B. anthracis into one of the 12 major sublineages or subgroups. All strains could be correctly positioned on the canonical SNP (canSNP) phylogenetic tree, except the divergent Pasteur vaccine strain ATCC 4229. We detected the cooccurrence of three canSNP subgroups in France. The dominant B.Br.CNEVA sublineage was found to be prevalent in the Alps, the Pyrenees, the Auvergne region, and the Saône-et-Loire department. Strains affiliated with the A.Br.008/009 subgroup were observed throughout most of the country. The minor A.Br.001/002 subgroup was restricted to northeastern France. Multiple-locus variable-number tandem-repeat analysis using 24 markers further resolved French strains into 60 unique profiles and identified some regional patterns. Diversity found within the A.Br.008/009 and B.Br.CNEVA subgroups suggests that these represent old, ecologically established clades in France. Phylogenetic relationships with strains from other parts of the world are discussed. PMID:21998431

  14. Calibration uncertainty

    DEFF Research Database (Denmark)

    Heydorn, Kaj; Anglov, Thomas

    2002-01-01

    uncertainty was verified from independent measurements of the same sample by demonstrating statistical control of analytical results and the absence of bias. The proposed method takes into account uncertainties of the measurement, as well as of the amount of calibrant. It is applicable to all types......Methods recommended by the International Standardization Organisation and Eurachem are not satisfactory for the correct estimation of calibration uncertainty. A novel approach is introduced and tested on actual calibration data for the determination of Pb by ICP-AES. The improved calibration...

  15. Incidence in diverse pig populations of an IGF2 mutation with potential influence on meat quality and quantity: An assay based on real time PCR (RT-PCR).

    Science.gov (United States)

    Carrodeguas, José Alberto; Burgos, Carmen; Moreno, Carlos; Sánchez, Ana Cristina; Ventanas, Sonia; Tarrafeta, Luis; Barcelona, José Antonio; López, Maria Otilia; Oria, Rosa; López-Buesa, Pascual

    2005-11-01

    IGF2, insulin-like growth factor 2, is implicated in myogenesis and lean meat content. A mutation in a single base (A for G substitution) of the gene for IGF2 (position 3072 in intron 3) has been recently described as the cause of a major QTL effect on muscle growth in pigs [Van Laere, A. S, Nguyen, M., Braunschweig, M., Nezer, C., Collete, C., & Moreau, L. et al. (2003). Nature, 425, 832-836]. We describe here a rapid assay based on real time PCR (RT-PCR) to detect this mutation. We have evaluated the incidence of the mutation in commercial pig crosses, in three populations of purebred Iberian or Iberian×Duroc crosses, and in cured meat products and wild boars. The incidence of the mutation varies among these groups. Penetrance of the A mutation is about 80% in the commercial population. Purebred Iberian pigs were all homozygous G/G whereas crosses of Iberian pigs were heterozygous (90%) or homozygous A/A (10%). The implications of this gene for the selection of Iberian pigs are discussed.

  16. Traceable Pyrgeometer Calibrations

    Energy Technology Data Exchange (ETDEWEB)

    Dooraghi, Mike; Kutchenreiter, Mark; Reda, Ibrahim; Habte, Aron; Sengupta, Manajit; Andreas, Afshin; Newman, Martina

    2016-05-02

    This poster presents the development, implementation, and operation of the Broadband Outdoor Radiometer Calibrations (BORCAL) Longwave (LW) system at the Southern Great Plains Radiometric Calibration Facility for the calibration of pyrgeometers that provide traceability to the World Infrared Standard Group.

  17. Colour calibration for colour reproduction

    OpenAIRE

    Emmel, P.; R. D. Hersch

    2000-01-01

    Due to the proliferation of low-cost colour devices (digital colour cameras, scanners, printers etc.) during the last few years, colour calibration has become an important issue. Such devices should faithfully reproduce colour images, but experience shows they don't. Among the main reasons, we note the diversity of acquisition, display and printing technologies which makes standardization difficult. Each device has a different gamut, i.e. a different set of colours that it can acquire or repr...

  18. Requirements for the accreditation of a calibration laboratory

    International Nuclear Information System (INIS)

    CNEA's activity in calibration is recent but it has a significant development. To assure high quality results, activity must be sustained and improved from day to day. The calibrations laboratory was accredited before Laboratories Qualification Committee, thus adding reliability to its results and making it more competitive when compared to other laboratories not accredited. Among other services given are supervision and follow up of calibrations in laboratories, participation in interlaboratory assays together with other calibration laboratories and assessments on calibration aspects of measuring equipment. (author)

  19. Standard guide for making quality nondestructive assay measurements

    CERN Document Server

    American Society for Testing and Materials. Philadelphia

    2009-01-01

    1.1 This guide is a compendium of Quality Measurement Practices for performing measurements of radioactive material using nondestructive assay (NDA) instruments. The primary purpose of the guide is to assist users in arriving at quality NDA results, that is, results that satisfy the end user’s needs. This is accomplished by providing an acceptable and uniform basis for the collection, analysis, comparison, and application of data. The recommendations are not compulsory or prerequisites to achieving quality NDA measurements, but are considered contributory in most areas. 1.2 This guide applies to the use of NDA instrumentation for the measurement of nuclear materials by the observation of spontaneous or stimulated nuclear radiations, including photons, neutrons, or the flow of heat. Recommended calibration, operating, and assurance methods represent guiding principles based on current NDA technology. The diversity of industry-wide nuclear materials measurement applications and instrumentation precludes disc...

  20. Angiogenesis Assays.

    Science.gov (United States)

    Nambiar, Dhanya K; Kujur, Praveen K; Singh, Rana P

    2016-01-01

    Neoangiogenesis constitutes one of the first steps of tumor progression beyond a critical size of tumor growth, which supplies a dormant mass of cancerous cells with the required nutrient supply and gaseous exchange through blood vessels essentially needed for their sustained and aggressive growth. In order to understand any biological process, it becomes imperative that we use models, which could mimic the actual biological system as closely as possible. Hence, finding the most appropriate model is always a vital part of any experimental design. Angiogenesis research has also been much affected due to lack of simple, reliable, and relevant models which could be easily quantitated. The angiogenesis models have been used extensively for studying the action of various molecules for agonist or antagonistic behaviour and associated mechanisms. Here, we have described two protocols or models which have been popularly utilized for studying angiogenic parameters. Rat aortic ring assay tends to bridge the gap between in vitro and in vivo models. The chorioallantoic membrane (CAM) assay is one of the most utilized in vivo model system for angiogenesis-related studies. The CAM is highly vascularized tissue of the avian embryo and serves as a good model to study the effects of various test compounds on neoangiogenesis. PMID:26608294

  1. Trinocular Calibration Method Based on Binocular Calibration

    Directory of Open Access Journals (Sweden)

    CAO Dan-Dan

    2012-10-01

    Full Text Available In order to solve the self-occlusion problem in plane-based multi-camera calibration system and expand the measurement range, a tri-camera vision system based on binocular calibration is proposed. The three cameras are grouped into two pairs, while the public camera is taken as the reference to build the global coordinate. By calibration of the measured absolute distance and the true absolute distance, global calibration is realized. The MRE (mean relative error of the global calibration of the two camera pairs in the experiments can be as low as 0.277% and 0.328% respectively. Experiment results show that this method is feasible, simple and effective, and has high precision.

  2. ORNL calibrations facility

    International Nuclear Information System (INIS)

    The ORNL Calibrations Facility is operated by the Instrumentation Group of the Industrial Safety and Applied Health Physics Division. Its primary purpose is to maintain radiation calibration standards for calibration of ORNL health physics instruments and personnel dosimeters. This report includes a discussion of the radioactive sources and ancillary equipment in use and a step-by-step procedure for calibration of those survey instruments and personnel dosimeters in routine use at ORNL

  3. Spiral reader calibration

    International Nuclear Information System (INIS)

    The method to calibrate the spiral reader (SR) is presented. A brief description of the main procedures of the calibration program SCALP, adapted for the IHEP equipment and purposes, is described. The precision characteristics of the IHEP SR have been analysed on the results, presented in the form of diagrams. There is a calibration manual for the user

  4. Residual gas analyzer calibration

    Science.gov (United States)

    Lilienkamp, R. H.

    1972-01-01

    A technique which employs known gas mixtures to calibrate the residual gas analyzer (RGA) is described. The mass spectra from the RGA are recorded for each gas mixture. This mass spectra data and the mixture composition data each form a matrix. From the two matrices the calibration matrix may be computed. The matrix mathematics requires the number of calibration gas mixtures be equal to or greater than the number of gases included in the calibration. This technique was evaluated using a mathematical model of an RGA to generate the mass spectra. This model included shot noise errors in the mass spectra. Errors in the gas concentrations were also included in the valuation. The effects of these errors was studied by varying their magnitudes and comparing the resulting calibrations. Several methods of evaluating an actual calibration are presented. The effects of the number of gases in then, the composition of the calibration mixture, and the number of mixtures used are discussed.

  5. OLI Radiometric Calibration

    Science.gov (United States)

    Markham, Brian; Morfitt, Ron; Kvaran, Geir; Biggar, Stuart; Leisso, Nathan; Czapla-Myers, Jeff

    2011-01-01

    Goals: (1) Present an overview of the pre-launch radiance, reflectance & uniformity calibration of the Operational Land Imager (OLI) (1a) Transfer to orbit/heliostat (1b) Linearity (2) Discuss on-orbit plans for radiance, reflectance and uniformity calibration of the OLI

  6. The GERDA calibration system

    International Nuclear Information System (INIS)

    A system with three identical custom made units is used for the energy calibration of the GERDA Ge diodes. To perform a calibration the 228Th sources are lowered from the parking positions at the top of the cryostat. Their positions are measured by two independent modules. One, the incremental encoder, counts the holes in the perforated steel band holding the sources, the other measures the drive shaft's angular position even if not powered. The system can be controlled remotely by a Labview program. The calibration data is analyzed by an iterative calibration algorithm determining the calibration functions for different energy reconstruction algorithms and the resolution of several peaks in the 228Th spectrum is determined. A Monte Carlo simulation using the GERDA simulation software MAGE has been performed to determine the background induced by the sources in the parking positions.

  7. Sandia WIPP calibration traceability

    International Nuclear Information System (INIS)

    This report summarizes the work performed to establish calibration traceability for the instrumentation used by Sandia National Laboratories at the Waste Isolation Pilot Plant (WIPP) during testing from 1980-1985. Identifying the calibration traceability is an important part of establishing a pedigree for the data and is part of the qualification of existing data. In general, the requirement states that the calibration of Measuring and Test equipment must have a valid relationship to nationally recognized standards or the basis for the calibration must be documented. Sandia recognized that just establishing calibration traceability would not necessarily mean that all QA requirements were met during the certification of test instrumentation. To address this concern, the assessment was expanded to include various activities

  8. Calibration of X-ray densitometers for the determination of uranium and plutonium concentrations in reprocessing input and product solutions

    International Nuclear Information System (INIS)

    In June 1985 a calibration exercise has been carried out, which included the calibration of the KfK K-Edge Densitometer for uranium assay in the uranium product solutions from reprocessing, and the calibration of the Hybrid K-Edge/K-XRF Instrument for the determination of total uranium and plutonium in reprocessing input solutions. The calibration measuremnts performed with the two X-ray densitometers are described and analyzed, and calibration constants are evaluated from the obtained results. (orig.)

  9. Segment Based Camera Calibration

    Institute of Scientific and Technical Information of China (English)

    马颂德; 魏国庆; 等

    1993-01-01

    The basic idea of calibrating a camera system in previous approaches is to determine camera parmeters by using a set of known 3D points as calibration reference.In this paper,we present a method of camera calibration in whih camera parameters are determined by a set of 3D lines.A set of constraints is derived on camea parameters in terms of perspective line mapping.Form these constraints,the same perspective transformation matrix as that for point mapping can be computed linearly.The minimum number of calibration lines is 6.This result generalizes that of Liu,Huang and Faugeras[12] for camera location determination in which at least 8 line correspondences are required for linear computation of camera location.Since line segments in an image can be located easily and more accurately than points,the use of lines as calibration reference tends to ease the computation in inage preprocessing and to improve calibration accuracy.Experimental results on the calibration along with stereo reconstruction are reported.

  10. Lidar Calibration Centre

    Science.gov (United States)

    Pappalardo, Gelsomina; Freudenthaler, Volker; Nicolae, Doina; Mona, Lucia; Belegante, Livio; D'Amico, Giuseppe

    2016-06-01

    This paper presents the newly established Lidar Calibration Centre, a distributed infrastructure in Europe, whose goal is to offer services for complete characterization and calibration of lidars and ceilometers. Mobile reference lidars, laboratories for testing and characterization of optics and electronics, facilities for inspection and debugging of instruments, as well as for training in good practices are open to users from the scientific community, operational services and private sector. The Lidar Calibration Centre offers support for trans-national access through the EC HORIZON2020 project ACTRIS-2.

  11. Equipment for dosemeter calibration

    International Nuclear Information System (INIS)

    The device is used for precise calibration of dosimetric instrumentation, such as used at nuclear facilities. The high precision of the calibration procedure is primarily due to the fact that one single and steady radiation source is used. The accurate alignment of the source and the absence of shielding materials in the beam axis make for high homogeneity of the beam and reproducibility of the measurement; this is also contributed to by the horizontal displacement of the optical bench, which ensures a constant temperature field and the possibility of adjusting the radiation source at a sufficient distance from the instrument to be calibrated. (Z.S.). 3 figs

  12. Input calibration for negative originals

    Science.gov (United States)

    Tuijn, Chris

    1995-04-01

    One of the major challenges in the prepress environment consists of controlling the electronic color reproduction process such that a perfect match of any original can be realized. Whether this goal can be reached depends on many factors such as the dynamic range of the input device (scanner, camera), the color gamut of the output device (dye sublimation printer, ink-jet printer, offset), the color management software etc. The characterization of the color behavior of the peripheral devices is therefore very important. Photographs and positive transparents reflect the original scene pretty well; for negative originals, however, there is no obvious link to either the original scene or a particular print of the negative under consideration. In this paper, we establish a method to scan negatives and to convert the scanned data to a calibrated RGB space, which is known colorimetrically. This method is based on the reconstruction of the original exposure conditions (i.e., original scene) which generated the negative. Since the characteristics of negative film are quite diverse, a special calibration is required for each combination of scanner and film type.

  13. SPOTS Calibration Example

    Directory of Open Access Journals (Sweden)

    Patterson E.

    2010-06-01

    Full Text Available The results are presented using the procedure outlined by the Standardisation Project for Optical Techniques of Strain measurement to calibrate a digital image correlation system. The process involves comparing the experimental data obtained with the optical measurement system to the theoretical values for a specially designed specimen. The standard states the criteria which must be met in order to achieve successful calibration, in addition to quantifying the measurement uncertainty in the system. The system was evaluated at three different displacement load levels, generating strain ranges from 289 µstrain to 2110 µstrain. At the 289 µstrain range, the calibration uncertainty was found to be 14.1 µstrain, and at the 2110 µstrain range it was found to be 28.9 µstrain. This calibration procedure was performed without painting a speckle pattern on the surface of the metal. Instead, the specimen surface was prepared using different grades of grit paper to produce the desired texture.

  14. Traceable Pyrgeometer Calibrations

    Energy Technology Data Exchange (ETDEWEB)

    Dooraghi, Mike; Kutchenreiter, Mark; Reda, Ibrahim; Habte, Aron; Sengupta, Manajit; Andreas, Afshin; Newman, Martina; Webb, Craig

    2016-05-02

    This presentation provides a high-level overview of the progress on the Broadband Outdoor Radiometer Calibrations for all shortwave and longwave radiometers that are deployed by the Atmospheric Radiation Measurement program.

  15. Air Data Calibration Facility

    Data.gov (United States)

    Federal Laboratory Consortium — This facility is for low altitude subsonic altimeter system calibrations of air vehicles. Mission is a direct support of the AFFTC mission. Postflight data merge is...

  16. Approximation Behooves Calibration

    DEFF Research Database (Denmark)

    da Silva Ribeiro, André Manuel; Poulsen, Rolf

    2013-01-01

    Calibration based on an expansion approximation for option prices in the Heston stochastic volatility model gives stable, accurate, and fast results for S&P500-index option data over the period 2005–2009.......Calibration based on an expansion approximation for option prices in the Heston stochastic volatility model gives stable, accurate, and fast results for S&P500-index option data over the period 2005–2009....

  17. Scanner calibration revisited

    Directory of Open Access Journals (Sweden)

    Pozhitkov Alexander E

    2010-07-01

    Full Text Available Abstract Background Calibration of a microarray scanner is critical for accurate interpretation of microarray results. Shi et al. (BMC Bioinformatics, 2005, 6, Art. No. S11 Suppl. 2. reported usage of a Full Moon BioSystems slide for calibration. Inspired by the Shi et al. work, we have calibrated microarray scanners in our previous research. We were puzzled however, that most of the signal intensities from a biological sample fell below the sensitivity threshold level determined by the calibration slide. This conundrum led us to re-investigate the quality of calibration provided by the Full Moon BioSystems slide as well as the accuracy of the analysis performed by Shi et al. Methods Signal intensities were recorded on three different microarray scanners at various photomultiplier gain levels using the same calibration slide from Full Moon BioSystems. Data analysis was conducted on raw signal intensities without normalization or transformation of any kind. Weighted least-squares method was used to fit the data. Results We found that initial analysis performed by Shi et al. did not take into account autofluorescence of the Full Moon BioSystems slide, which led to a grossly distorted microarray scanner response. Our analysis revealed that a power-law function, which is explicitly accounting for the slide autofluorescence, perfectly described a relationship between signal intensities and fluorophore quantities. Conclusions Microarray scanners respond in a much less distorted fashion than was reported by Shi et al. Full Moon BioSystems calibration slides are inadequate for performing calibration. We recommend against using these slides.

  18. Energy calibration via correlation

    CERN Document Server

    Maier, Daniel

    2015-01-01

    The main task of an energy calibration is to find a relation between pulse-height values and the corresponding energies. Doing this for each pulse-height channel individually requires an elaborated input spectrum with an excellent counting statistics and a sophisticated data analysis. This work presents an easy to handle energy calibration process which can operate reliably on calibration measurements with low counting statistics. The method uses a parameter based model for the energy calibration and concludes on the optimal parameters of the model by finding the best correlation between the measured pulse-height spectrum and multiple synthetic pulse-height spectra which are constructed with different sets of calibration parameters. A CdTe-based semiconductor detector and the line emissions of an 241 Am source were used to test the performance of the correlation method in terms of systematic calibration errors for different counting statistics. Up to energies of 60 keV systematic errors were measured to be le...

  19. Calibrating nacelle lidars

    Energy Technology Data Exchange (ETDEWEB)

    Courtney, M.

    2013-01-15

    Nacelle mounted, forward looking wind lidars are beginning to be used to provide reference wind speed measurements for the power performance testing of wind turbines. In such applications, a formal calibration procedure with a corresponding uncertainty assessment will be necessary. This report presents four concepts for performing such a nacelle lidar calibration. Of the four methods, two are found to be immediately relevant and are pursued in some detail. The first of these is a line of sight calibration method in which both lines of sight (for a two beam lidar) are individually calibrated by accurately aligning the beam to pass close to a reference wind speed sensor. A testing procedure is presented, reporting requirements outlined and the uncertainty of the method analysed. It is seen that the main limitation of the line of sight calibration method is the time required to obtain a representative distribution of radial wind speeds. An alternative method is to place the nacelle lidar on the ground and incline the beams upwards to bisect a mast equipped with reference instrumentation at a known height and range. This method will be easier and faster to implement and execute but the beam inclination introduces extra uncertainties. A procedure for conducting such a calibration is presented and initial indications of the uncertainties given. A discussion of the merits and weaknesses of the two methods is given together with some proposals for the next important steps to be taken in this work. (Author)

  20. Performance Testing of PCR Assay in Blood Samples for the Diagnosis of Toxoplasmic Encephalitis in AIDS Patients from the French Departments of America and Genetic Diversity of Toxoplasma gondii: A Prospective and Multicentric Study

    Science.gov (United States)

    Ajzenberg, Daniel; Lamaury, Isabelle; Demar, Magalie; Vautrin, Cyrille; Cabié, André; Simon, Stéphane; Nicolas, Muriel; Desbois-Nogard, Nicole; Boukhari, Rachida; Riahi, Homayoun; Dardé, Marie-Laure; Massip, Patrice; Dupon, Michel; Preux, Pierre-Marie; Labrunie, Anaïs; Boncoeur, Marie-Paule

    2016-01-01

    Background Toxoplasmic encephalitis in patients with AIDS is a life-threatening disease mostly due to reactivation of Toxoplasma gondii cysts in the brain. The main objective of this study was to evaluate the performance of real-time PCR assay in peripheral blood samples for the diagnosis of toxoplasmic encephalitis in AIDS patients in the French West Indies and Guiana. Methodology/Principal Findings Adult patients with HIV and suspicion of toxoplasmic encephalitis with start of specific antitoxoplasmic therapy were included in this study during 40 months. The real-time PCR assay targeting the 529 bp repeat region of T. gondii was performed in two different centers for all blood samples. A Neighbor-Joining tree was reconstructed from microsatellite data to examine the relationships between strains from human cases of toxoplasmosis in South America and the Caribbean. A total of 44 cases were validated by a committee of experts, including 36 cases with toxoplasmic encephalitis. The specificity of the PCR assay in blood samples was 100% but the sensitivity was only 25% with moderate agreement between the two centers. Altered level of consciousness and being born in the French West Indies and Guiana were the only two variables that were associated with significantly decreased risk of false negative results with the PCR assay. Conclusion/Significance Our results showed that PCR sensitivity in blood samples increased with severity of toxoplasmic encephalitis in AIDS patients. Geographic origin of patients was likely to influence PCR sensitivity but there was little evidence that it was caused by differences in T. gondii strains. Trial Registration ClinicalTrials.gov NCT00803621 PMID:27355620

  1. Performance Testing of PCR Assay in Blood Samples for the Diagnosis of Toxoplasmic Encephalitis in AIDS Patients from the French Departments of America and Genetic Diversity of Toxoplasma gondii: A Prospective and Multicentric Study.

    Directory of Open Access Journals (Sweden)

    Daniel Ajzenberg

    2016-06-01

    Full Text Available Toxoplasmic encephalitis in patients with AIDS is a life-threatening disease mostly due to reactivation of Toxoplasma gondii cysts in the brain. The main objective of this study was to evaluate the performance of real-time PCR assay in peripheral blood samples for the diagnosis of toxoplasmic encephalitis in AIDS patients in the French West Indies and Guiana.Adult patients with HIV and suspicion of toxoplasmic encephalitis with start of specific antitoxoplasmic therapy were included in this study during 40 months. The real-time PCR assay targeting the 529 bp repeat region of T. gondii was performed in two different centers for all blood samples. A Neighbor-Joining tree was reconstructed from microsatellite data to examine the relationships between strains from human cases of toxoplasmosis in South America and the Caribbean. A total of 44 cases were validated by a committee of experts, including 36 cases with toxoplasmic encephalitis. The specificity of the PCR assay in blood samples was 100% but the sensitivity was only 25% with moderate agreement between the two centers. Altered level of consciousness and being born in the French West Indies and Guiana were the only two variables that were associated with significantly decreased risk of false negative results with the PCR assay.Our results showed that PCR sensitivity in blood samples increased with severity of toxoplasmic encephalitis in AIDS patients. Geographic origin of patients was likely to influence PCR sensitivity but there was little evidence that it was caused by differences in T. gondii strains.ClinicalTrials.gov NCT00803621.

  2. Calibration of the TVO spent BWR reference fuel assembly

    International Nuclear Information System (INIS)

    In 1989 the Support Programmes of Finland (FSP) and Sweden (SSP) initiated a joint task to cross calibrate the burnup of the IAEA spent BWR reference fuel assembly at the TVO AFR storage facility (TVO KPA-STORE) in Finland. The reference assembly, kept separately under the IAEA seal, is used for verification measurements of spent fuel by GBUV method (SG-NDA-38). The cross calibration was performed by establishing a calibration curve, 244Cm neutron rate versus burnup, using passive neutron assay (PNA) measurements. The declared burnup of the reference assembly was compared with the burnup value deduced from the calibration curve. A calibration line was also established by using the GBUV method with the aid of high resolution gamma ray spectrometry (HRGS). Normalization between the two different facilities was performed using sealed neutron and gamma calibration sources. The results of the passive neutron assay show consistency, better than 1 %, between the declared mean burnup of the reference assembly and the burnup deduced from the calibration curve. The corresponding consistency is within +-2 % for the HRGS measurements

  3. Fast facility spent-fuel and waste assay instrument

    International Nuclear Information System (INIS)

    A delayed-neutron assay instrument was installed in the Fluorinel Dissolution and Fuel Storage Facility at Idaho National Engineering Laboratory. The dual-assay instrument is designed to measure both spent fuel and waste solids that are produced from fuel processing. A set of waste standards, fabricated by Los Alamos using uranium supplied by Exxon Nuclear Idaho Company, was used to calibrate the small-sample assay region of the instrument. Performance testing was completed before installation of the instrument to determine the effects of uranium enrichment, hydrogenous materials, and neutron poisons on assays. The unit was designed to measure high-enriched uranium samples in the presence of large neutron backgrounds. Measurements indicate that the system can assay low-enriched uranium samples with moderate backgrounds if calibrated with proper standards

  4. Managing diversity.

    Science.gov (United States)

    Epting, L A; Glover, S H; Boyd, S D

    1994-06-01

    The U.S. work force is becoming increasingly diverse as the 20th century approaches. Statistics prove that most organizations are experiencing gender, culture, and age diversity within their labor forces. All managers and leaders must accept this diversity and work to handle it effectively. This article examines the current literature concerning management of diversity and its implications for the health care profession. Gender, culture, and age diversity and the potential problems that may arise with each are also addressed. Reasons to manage diversity are offered, as well as methods of managing diversity for both the manager and the chief executive officer. PMID:10134144

  5. HAWC Timing Calibration

    CERN Document Server

    Huentemeyer, Petra; Dingus, Brenda

    2009-01-01

    The High-Altitude Water Cherenkov (HAWC) Experiment is a second-generation highsensitivity gamma-ray and cosmic-ray detector that builds on the experience and technology of the Milagro observatory. Like Milagro, HAWC utilizes the water Cherenkov technique to measure extensive air showers. Instead of a pond filled with water (as in Milagro) an array of closely packed water tanks is used. The event direction will be reconstructed using the times when the PMTs in each tank are triggered. Therefore, the timing calibration will be crucial for reaching an angular resolution as low as 0.25 degrees.We propose to use a laser calibration system, patterned after the calibration system in Milagro. Like Milagro, the HAWC optical calibration system will use ~1 ns laser light pulses. Unlike Milagro, the PMTs are optically isolated and require their own optical fiber calibration. For HAWC the laser light pulses will be directed through a series of optical fan-outs and fibers to illuminate the PMTs in approximately one half o...

  6. Calibration Under Uncertainty.

    Energy Technology Data Exchange (ETDEWEB)

    Swiler, Laura Painton; Trucano, Timothy Guy

    2005-03-01

    This report is a white paper summarizing the literature and different approaches to the problem of calibrating computer model parameters in the face of model uncertainty. Model calibration is often formulated as finding the parameters that minimize the squared difference between the model-computed data (the predicted data) and the actual experimental data. This approach does not allow for explicit treatment of uncertainty or error in the model itself: the model is considered the %22true%22 deterministic representation of reality. While this approach does have utility, it is far from an accurate mathematical treatment of the true model calibration problem in which both the computed data and experimental data have error bars. This year, we examined methods to perform calibration accounting for the error in both the computer model and the data, as well as improving our understanding of its meaning for model predictability. We call this approach Calibration under Uncertainty (CUU). This talk presents our current thinking on CUU. We outline some current approaches in the literature, and discuss the Bayesian approach to CUU in detail.

  7. Polarimetric Palsar Calibration

    Science.gov (United States)

    Touzi, R.; Shimada, M.

    2008-11-01

    Polarimetric PALSAR system parameters are assessed using data sets collected over various calibration sites. The data collected over the Amazonian forest permits validating the zero Faraday rotation hypotheses near the equator. The analysis of the Amazonian forest data and the response of the corner reflectors deployed during the PALSAR acquisitions lead to the conclusion that the antenna is highly isolated (better than -35 dB). Theses results are confirmed using data collected over the Sweden and Ottawa calibration sites. The 5-m height trihedrals deployed in the Sweden calibration site by the Chalmers University of technology permits accurate measurement of antenna parameters, and detection of 2-3 degree Faraday rotation during day acquisition, whereas no Faraday rotation was noted during night acquisition. Small Faraday rotation angles (2-3 degree) have been measured using acquisitions over the DLR Oberpfaffenhofen and the Ottawa calibration sites. The presence of small but still significant Faraday rotation (2-3 degree) induces a CR return at the cross-polarization HV and VH that should not be interpreted as the actual antenna cross-talk. PALSAR antenna is highly isolated (better than -35 dB), and diagonal antenna distortion matrices (with zero cross-talk terms) can be used for accurate calibration of PALSAR polarimetric data.

  8. GTC Photometric Calibration

    Science.gov (United States)

    di Cesare, M. A.; Hammersley, P. L.; Rodriguez Espinosa, J. M.

    2006-06-01

    We are currently developing the calibration programme for GTC using techniques similar to the ones use for the space telescope calibration (Hammersley et al. 1998, A&AS, 128, 207; Cohen et al. 1999, AJ, 117, 1864). We are planning to produce a catalogue with calibration stars which are suitable for a 10-m telescope. These sources will be not variable, non binary and do not have infrared excesses if they are to be used in the infrared. The GTC science instruments require photometric calibration between 0.35 and 2.5 microns. The instruments are: OSIRIS (Optical System for Imaging low Resolution Integrated Spectroscopy), ELMER and EMIR (Espectrógrafo Multiobjeto Infrarrojo) and the Acquisition and Guiding boxes (Di Césare, Hammersley, & Rodriguez Espinosa 2005, RevMexAA Ser. Conf., 24, 231). The catalogue will consist of 30 star fields distributed in all of North Hemisphere. We will use fields containing sources over the range 12 to 22 magnitude, and spanning a wide range of spectral types (A to M) for the visible and near infrared. In the poster we will show the method used for selecting these fields and we will present the analysis of the data on the first calibration fields observed.

  9. TARGETLESS CAMERA CALIBRATION

    Directory of Open Access Journals (Sweden)

    L. Barazzetti

    2012-09-01

    Full Text Available In photogrammetry a camera is considered calibrated if its interior orientation parameters are known. These encompass the principal distance, the principal point position and some Additional Parameters used to model possible systematic errors. The current state of the art for automated camera calibration relies on the use of coded targets to accurately determine the image correspondences. This paper presents a new methodology for the efficient and rigorous photogrammetric calibration of digital cameras which does not require any longer the use of targets. A set of images depicting a scene with a good texture are sufficient for the extraction of natural corresponding image points. These are automatically matched with feature-based approaches and robust estimation techniques. The successive photogrammetric bundle adjustment retrieves the unknown camera parameters and their theoretical accuracies. Examples, considerations and comparisons with real data and different case studies are illustrated to show the potentialities of the proposed methodology.

  10. The MINOS calibration detector

    International Nuclear Information System (INIS)

    This paper describes the MINOS calibration detector (CalDet) and the procedure used to calibrate it. The CalDet, a scaled-down but functionally equivalent model of the MINOS Far and Near detectors, was exposed to test beams in the CERN PS East Area during 2001-2003 to establish the response of the MINOS calorimeters to hadrons, electrons and muons in the range 0.2-10GeV/c. The CalDet measurements are used to fix the energy scale and constrain Monte Carlo simulations of MINOS

  11. Individual dosimetry and calibration

    International Nuclear Information System (INIS)

    In 1995 both the Individual Dosimetry and Calibration Sections worked under the condition of a status quo and concentrated fully on the routine part of their work. Nevertheless, the machine for printing the bar code which will be glued onto the film holder and hence identify the people when entering into high radiation areas was put into operation and most of the holders were equipped with the new identification. As far as the Calibration Section is concerned the project of the new source control system that is realized by the Technical Support Section was somewhat accelerated

  12. Marine X-band Weather Radar Data Calibration

    DEFF Research Database (Denmark)

    Thorndahl, Søren Liedtke; Rasmussen, Michael R.

    2012-01-01

    Application of weather radar data in urban hydrology is evolving and radar data is now applied for both modelling, analysis, and real time control purposes. In these contexts, it is allimportant that the radar data is well calibrated and adjusted in order to obtain valid quantitative precipitation...... estimates. This paper presents some of the challenges in small marine X-band radar calibration by comparing three calibration procedures for assessing the relationship between radar and rain gauge data. Validation shows similar results for precipitation volumes but more diverse results on peak rain...

  13. Entropic calibration revisited

    Energy Technology Data Exchange (ETDEWEB)

    Brody, Dorje C. [Blackett Laboratory, Imperial College, London SW7 2BZ (United Kingdom)]. E-mail: d.brody@imperial.ac.uk; Buckley, Ian R.C. [Centre for Quantitative Finance, Imperial College, London SW7 2AZ (United Kingdom); Constantinou, Irene C. [Blackett Laboratory, Imperial College, London SW7 2BZ (United Kingdom); Meister, Bernhard K. [Blackett Laboratory, Imperial College, London SW7 2BZ (United Kingdom)

    2005-04-11

    The entropic calibration of the risk-neutral density function is effective in recovering the strike dependence of options, but encounters difficulties in determining the relevant greeks. By use of put-call reversal we apply the entropic method to the time reversed economy, which allows us to obtain the spot price dependence of options and the relevant greeks.

  14. LOFAR Facet Calibration

    Science.gov (United States)

    van Weeren, R. J.; Williams, W. L.; Hardcastle, M. J.; Shimwell, T. W.; Rafferty, D. A.; Sabater, J.; Heald, G.; Sridhar, S. S.; Dijkema, T. J.; Brunetti, G.; Brüggen, M.; Andrade-Santos, F.; Ogrean, G. A.; Röttgering, H. J. A.; Dawson, W. A.; Forman, W. R.; de Gasperin, F.; Jones, C.; Miley, G. K.; Rudnick, L.; Sarazin, C. L.; Bonafede, A.; Best, P. N.; Bîrzan, L.; Cassano, R.; Chyży, K. T.; Croston, J. H.; Ensslin, T.; Ferrari, C.; Hoeft, M.; Horellou, C.; Jarvis, M. J.; Kraft, R. P.; Mevius, M.; Intema, H. T.; Murray, S. S.; Orrú, E.; Pizzo, R.; Simionescu, A.; Stroe, A.; van der Tol, S.; White, G. J.

    2016-03-01

    LOFAR, the Low-Frequency Array, is a powerful new radio telescope operating between 10 and 240 MHz. LOFAR allows detailed sensitive high-resolution studies of the low-frequency radio sky. At the same time LOFAR also provides excellent short baseline coverage to map diffuse extended emission. However, producing high-quality deep images is challenging due to the presence of direction-dependent calibration errors, caused by imperfect knowledge of the station beam shapes and the ionosphere. Furthermore, the large data volume and presence of station clock errors present additional difficulties. In this paper we present a new calibration scheme, which we name facet calibration, to obtain deep high-resolution LOFAR High Band Antenna images using the Dutch part of the array. This scheme solves and corrects the direction-dependent errors in a number of facets that cover the observed field of view. Facet calibration provides close to thermal noise limited images for a typical 8 hr observing run at ∼ 5\\prime\\prime resolution, meeting the specifications of the LOFAR Tier-1 northern survey.

  15. LOFAR facet calibration

    CERN Document Server

    van Weeren, R J; Hardcastle, M J; Shimwell, T W; Rafferty, D A; Sabater, J; Heald, G; Sridhar, S S; Dijkema, T J; Brunetti, G; Brüggen, M; Andrade-Santos, F; Ogrean, G A; Röttgering, H J A; Dawson, W A; Forman, W R; de Gasperin, F; Jones, C; Miley, G K; Rudnick, L; Sarazin, C L; Bonafede, A; Best, P N; Bîrzan, L; Cassano, R; Chyży, K T; Croston, J H; Ensslin, T; Ferrari, C; Hoeft, M; Horellou, C; Jarvis, M J; Kraft, R P; Mevius, M; Intema, H T; Murray, S S; Orrú, E; Pizzo, R; Simionescu, A; Stroe, A; van der Tol, S; White, G J

    2016-01-01

    LOFAR, the Low-Frequency Array, is a powerful new radio telescope operating between 10 and 240 MHz. LOFAR allows detailed sensitive high-resolution studies of the low-frequency radio sky. At the same time LOFAR also provides excellent short baseline coverage to map diffuse extended emission. However, producing high-quality deep images is challenging due to the presence of direction dependent calibration errors, caused by imperfect knowledge of the station beam shapes and the ionosphere. Furthermore, the large data volume and presence of station clock errors present additional difficulties. In this paper we present a new calibration scheme, which we name facet calibration, to obtain deep high-resolution LOFAR High Band Antenna images using the Dutch part of the array. This scheme solves and corrects the direction dependent errors in a number of facets that cover the observed field of view. Facet calibration provides close to thermal noise limited images for a typical 8 hr observing run at $\\sim$ 5arcsec resolu...

  16. Calibration of farmer dosemeters

    International Nuclear Information System (INIS)

    The Farmer Dosemeters of Atomic Energy Medical Centre (AEMC) Jamshoro were calibrated in the Secondary Standard Dosimetry Laboratory (SSDL) at PINSTECH, using the NPL Secondary Standard Therapy level X-ray exposure meter. The results are presented in this report. (authors)

  17. Calibration Of Oxygen Monitors

    Science.gov (United States)

    Zalenski, M. A.; Rowe, E. L.; Mcphee, J. R.

    1988-01-01

    Readings corrected for temperature, pressure, and humidity of air. Program for handheld computer developed to ensure accuracy of oxygen monitors in National Transonic Facility, where liquid nitrogen stored. Calibration values, determined daily, based on entries of data on barometric pressure, temperature, and relative humidity. Output provided directly in millivolts.

  18. Commodity-Free Calibration

    Science.gov (United States)

    2008-01-01

    Commodity-free calibration is a reaction rate calibration technique that does not require the addition of any commodities. This technique is a specific form of the reaction rate technique, where all of the necessary reactants, other than the sample being analyzed, are either inherent in the analyzing system or specifically added or provided to the system for a reason other than calibration. After introduction, the component of interest is exposed to other reactants or flow paths already present in the system. The instrument detector records one of the following to determine the rate of reaction: the increase in the response of the reaction product, a decrease in the signal of the analyte response, or a decrease in the signal from the inherent reactant. With this data, the initial concentration of the analyte is calculated. This type of system can analyze and calibrate simultaneously, reduce the risk of false positives and exposure to toxic vapors, and improve accuracy. Moreover, having an excess of the reactant already present in the system eliminates the need to add commodities, which further reduces cost, logistic problems, and potential contamination. Also, the calculations involved can be simplified by comparison to those of the reaction rate technique. We conducted tests with hypergols as an initial investigation into the feasiblility of the technique.

  19. Measurement System & Calibration report

    DEFF Research Database (Denmark)

    Kock, Carsten Weber; Vesth, Allan

    This Measurement System & Calibration report is describing DTU’s measurement system installed at a specific wind turbine. A major part of the sensors has been installed by others (see [1]) the rest of the sensors have been installed by DTU. The results of the measurements, described in this report...

  20. Calibration with Absolute Shrinkage

    DEFF Research Database (Denmark)

    Øjelund, Henrik; Madsen, Henrik; Thyregod, Poul

    2001-01-01

    In this paper, penalized regression using the L-1 norm on the estimated parameters is proposed for chemometric je calibration. The algorithm is of the lasso type, introduced by Tibshirani in 1996 as a linear regression method with bound on the absolute length of the parameters, but a modification...

  1. NVLAP calibration laboratory program

    Energy Technology Data Exchange (ETDEWEB)

    Cigler, J.L.

    1993-12-31

    This paper presents an overview of the progress up to April 1993 in the development of the Calibration Laboratories Accreditation Program within the framework of the National Voluntary Laboratory Accreditation Program (NVLAP) at the National Institute of Standards and Technology (NIST).

  2. Pleiades Absolute Calibration : Inflight Calibration Sites and Methodology

    Science.gov (United States)

    Lachérade, S.; Fourest, S.; Gamet, P.; Lebègue, L.

    2012-07-01

    In-flight calibration of space sensors once in orbit is a decisive step to be able to fulfil the mission objectives. This article presents the methods of the in-flight absolute calibration processed during the commissioning phase. Four In-flight calibration methods are used: absolute calibration, cross-calibration with reference sensors such as PARASOL or MERIS, multi-temporal monitoring and inter-bands calibration. These algorithms are based on acquisitions over natural targets such as African deserts, Antarctic sites, La Crau (Automatic calibration station) and Oceans (Calibration over molecular scattering) or also new extra-terrestrial sites such as the Moon and selected stars. After an overview of the instrument and a description of the calibration sites, it is pointed out how each method is able to address one or several aspects of the calibration. We focus on how these methods complete each other in their operational use, and how they help building a coherent set of information that addresses all aspects of in-orbit calibration. Finally, we present the perspectives that the high level of agility of PLEIADES offers for the improvement of its calibration and a better characterization of the calibration sites.

  3. Mercury CEM Calibration

    Energy Technology Data Exchange (ETDEWEB)

    John F. Schabron; Joseph F. Rovani; Susan S. Sorini

    2007-03-31

    The Clean Air Mercury Rule (CAMR) which was published in the Federal Register on May 18, 2005, requires that calibration of mercury continuous emissions monitors (CEMs) be performed with NIST-traceable standards. Western Research Institute (WRI) is working closely with the Electric Power Research Institute (EPRI), the National Institute of Standards and Technology (NIST), and the Environmental Protection Agency (EPA) to facilitate the development of the experimental criteria for a NIST traceability protocol for dynamic elemental mercury vapor generators. The traceability protocol will be written by EPA. Traceability will be based on the actual analysis of the output of each calibration unit at several concentration levels ranging from about 2-40 ug/m{sup 3}, and this analysis will be directly traceable to analyses by NIST using isotope dilution inductively coupled plasma/mass spectrometry (ID ICP/MS) through a chain of analyses linking the calibration unit in the power plant to the NIST ID ICP/MS. Prior to this project, NIST did not provide a recommended mercury vapor pressure equation or list mercury vapor pressure in its vapor pressure database. The NIST Physical and Chemical Properties Division in Boulder, Colorado was subcontracted under this project to study the issue in detail and to recommend a mercury vapor pressure equation that the vendors of mercury vapor pressure calibration units can use to calculate the elemental mercury vapor concentration in an equilibrium chamber at a particular temperature. As part of this study, a preliminary evaluation of calibration units from five vendors was made. The work was performed by NIST in Gaithersburg, MD and Joe Rovani from WRI who traveled to NIST as a Visiting Scientist.

  4. Field calibration of cup anemometers

    DEFF Research Database (Denmark)

    Schmidt Paulsen, Uwe; Mortensen, Niels Gylling; Hansen, Jens Carsten;

    2007-01-01

    A field calibration method and results are described along with the experience gained with the method. The cup anemometers to be calibrated are mounted in a row on a 10-m high rig and calibrated in the free wind against a reference cup anemometer. The method has been reported [1] to improve the...... statistical bias on the data relative to calibrations carried out in a wind tunnel. The methodology is sufficiently accurate for calibration of cup anemometers used for wind resource assessments and provides a simple, reliable and cost-effective solution to cup anemometer calibration, especially suited for...

  5. Diversity management

    OpenAIRE

    Horázná, Eliška

    2011-01-01

    The thesis is focused on diversity management in the Czech Republic. The author deals with diversity of economically active population in the Czech Republic and also, based on the screening of websites of selected companies, analyses the extent to which companies in the Czech Republic deal with diversity management in the context of personal marketing. Emphasis is placed on how the studied companies present their approaches to diversity and whether it is also used in individual job offers. Th...

  6. Embracing Diversity

    NARCIS (Netherlands)

    S. Puntoni (Stefano)

    2015-01-01

    markdownabstract__Abstract__ Societies are vastly more diverse today than they used to be and, in many industries, developing theories and approaches that recognize and capitalize on this greater consumer diversity is crucial. In business schools, diversity tends to be discussed only in relation to

  7. Development of an integrated assay facility

    International Nuclear Information System (INIS)

    The I.R.I.S. concept proposed the use of passive examination and active interrogation techniques in an integrated assay facility. A linac would generate the interrogating gamma and neutron beams. Insufficiently detailed knowledge about active neutron and gamma interrogation of 500 litre drums of cement immobilised intermediate level waste led to a research programme which is now in its main experimental stage. Measurements of interrogation responses are being made using simulated waste drums containing actinide samples and calibration sources, in an experimental assay assembly. Results show that responses are generally consistent with theory, but that improvements are needed in some areas. A preliminary appraisal of the engineering and economic aspects of integrated assay shows that correct operational sequencing is required to achieve the short cycle time needed for high throughput. The main engineering features of a facility have been identified

  8. Photometric calibrations for 21st century science

    Energy Technology Data Exchange (ETDEWEB)

    Kent, Stephen; /Fermilab; Kaiser, Mary Elizabeth; /Johns Hopkins U.; Deustua, Susana E.; /Baltimore, Space Telescope Sci.; Smith, J.Allyn; /Austin Peay State U.; Adelman, Saul; /Citadel Military Coll.; Allam, Sahar S.; /Fermilab; Baptista, Brian; /Indiana U.; Bohlin, Ralph C.; /Baltimore, Space Telescope Sci.; Clem, James L.; /Louisiana State U.; Conley, Alex; /Colorado U.; Edelstein, Jerry; /UC, Berkeley, Space Sci. Dept. /NOAO, Tucson

    2009-02-01

    The answers to fundamental science questions in astrophysics, ranging from the history of the expansion of the universe to the sizes of nearby stars, hinge on our ability to make precise measurements of diverse astronomical objects. As our knowledge of the underlying physics of objects improves along with advances in detectors and instrumentation, the limits on our capability to extract science from measurements is set, not by our lack of understanding of the nature of these objects, but rather by the most mundane of all issues: the precision with which we can calibrate observations in physical units. In principle, photometric calibration is a solved problem - laboratory reference standards such as blackbody furnaces achieve precisions well in excess of those needed for astrophysics. In practice, however, transferring the calibration from these laboratory standards to astronomical objects of interest is far from trivial - the transfer must reach outside the atmosphere, extend over 4{pi} steradians of sky, cover a wide range of wavelengths, and span an enormous dynamic range in intensity. Virtually all spectrophotometric observations today are calibrated against one or more stellar reference sources, such as Vega, which are themselves tied back to laboratory standards in a variety of ways. This system's accuracy is not uniform. Selected regions of the electromagnetic spectrum are calibrated extremely well, but discontinuities of a few percent still exist, e.g., between the optical and infrared. Independently, model stellar atmospheres are used to calibrate the spectra of selected white dwarf stars, e.g. the HST system, but the ultimate accuracy of this system should be verified against laboratory sources. Our traditional standard star systems, while sufficient until now, need to be improved and extended in order to serve future astrophysics experiments. This white paper calls for a program to improve upon and expand the current networks of

  9. Polarimetric calibration of large mirrors

    CERN Document Server

    Ariste, A Lopez

    2015-01-01

    Aims: To propose a method for the polarimetric calibration of large astronomical mirrors that does not require use of special optical devices nor knowledge of the exact polarization properties of the calibration target. Methods: We study the symmetries of the Mueller matrix of mirrors to exploit them for polarimetric calibration under the assumptions that only the orientation of the linear polarization plane of the calibration target is known with certainty. Results: A method is proposed to calibrate the polarization effects of single astronomical mirrors by the observation of calibration targets with known orientation of the linear polarization. We study the uncertainties of the method and the signal-to-noise ratios required for an acceptable calibration. We list astronomical targets ready for the method. We finally extend the method to the calibration of two or more mirrors, in particular to the case when they share the same incidence plane.

  10. The Calibration Reference Data System

    Science.gov (United States)

    Greenfield, P.; Miller, T.

    2016-07-01

    We describe a software architecture and implementation for using rules to determine which calibration files are appropriate for calibrating a given observation. This new system, the Calibration Reference Data System (CRDS), replaces what had been previously used for the Hubble Space Telescope (HST) calibration pipelines, the Calibration Database System (CDBS). CRDS will be used for the James Webb Space Telescope (JWST) calibration pipelines, and is currently being used for HST calibration pipelines. CRDS can be easily generalized for use in similar applications that need a rules-based system for selecting the appropriate item for a given dataset; we give some examples of such generalizations that will likely be used for JWST. The core functionality of the Calibration Reference Data System is available under an Open Source license. CRDS is briefly contrasted with a sampling of other similar systems used at other observatories.

  11. Lidar calibration experiments

    DEFF Research Database (Denmark)

    Ejsing Jørgensen, Hans; Mikkelsen, T.; Streicher, J.;

    1997-01-01

    A series of atmospheric aerosol diffusion experiments combined with lidar detection was conducted to evaluate and calibrate an existing retrieval algorithm for aerosol backscatter lidar systems. The calibration experiments made use of two (almost) identical mini-lidar systems for aerosol cloud...... detection to test the reproducibility and uncertainty of lidars. Lidar data were obtained from both single-ended and double-ended Lidar configurations. A backstop was introduced in one of the experiments and a new method was developed where information obtained from the backstop can be used in the inversion...... algorithm. Independent in-situ aerosol plume concentrations were obtained from a simultaneous tracer gas experiment with SF6, and comparisons with the two lidars were made. The study shows that the reproducibility of the lidars is within 15%, including measurements from both sides of a plume...

  12. Optical tweezers absolute calibration

    CERN Document Server

    Dutra, R S; Neto, P A Maia; Nussenzveig, H M

    2014-01-01

    Optical tweezers are highly versatile laser traps for neutral microparticles, with fundamental applications in physics and in single molecule cell biology. Force measurements are performed by converting the stiffness response to displacement of trapped transparent microspheres, employed as force transducers. Usually, calibration is indirect, by comparison with fluid drag forces. This can lead to discrepancies by sizable factors. Progress achieved in a program aiming at absolute calibration, conducted over the past fifteen years, is briefly reviewed. Here we overcome its last major obstacle, a theoretical overestimation of the peak stiffness, within the most employed range for applications, and we perform experimental validation. The discrepancy is traced to the effect of primary aberrations of the optical system, which are now included in the theory. All required experimental parameters are readily accessible. Astigmatism, the dominant effect, is measured by analyzing reflected images of the focused laser spo...

  13. Microbead agglutination based assays

    KAUST Repository

    Kodzius, Rimantas

    2013-01-21

    We report a simple and rapid room temperature assay for point-of-care (POC) testing that is based on specific agglutination. Agglutination tests are based on aggregation of microbeads in the presence of a specific analyte thus enabling the macroscopic observation. Such tests are most often used to explore antibody-antigen reactions. Agglutination has been used for protein assays using a biotin/streptavidin system as well as a hybridization based assay. The agglutination systems are prone to selftermination of the linking analyte, prone to active site saturation and loss of agglomeration at high analyte concentrations. We investigated the molecular target/ligand interaction, explaining the common agglutination problems related to analyte self-termination, linkage of the analyte to the same bead instead of different microbeads. We classified the agglutination process into three kinds of assays: a two- component assay, a three-component assay and a stepped three- component assay. Although we compared these three kinds of assays for recognizing DNA and protein molecules, the assay can be used for virtually any molecule, including ions and metabolites. In total, the optimized assay permits detecting analytes with high sensitivity in a short time, 5 min, at room temperature. Such a system is appropriate for POC testing.

  14. Astrid-2 SSC ASUMagnetic Calibration

    DEFF Research Database (Denmark)

    Primdahl, Fritz

    1997-01-01

    Report of the inter calibration between the starcamera and the fluxgate magnetometer onboard the ASTRID-2 satellite. This calibration was performed in the night between the 15. and 16. May 1997 at the Lovö magnetic observatory.......Report of the inter calibration between the starcamera and the fluxgate magnetometer onboard the ASTRID-2 satellite. This calibration was performed in the night between the 15. and 16. May 1997 at the Lovö magnetic observatory....

  15. Program Calibrates Strain Gauges

    Science.gov (United States)

    Okazaki, Gary D.

    1991-01-01

    Program dramatically reduces personnel and time requirements for acceptance tests of hardware. Data-acquisition system reads output from Wheatstone full-bridge strain-gauge circuit and calculates strain by use of shunt calibration technique. Program nearly instantaneously tabulates and plots strain data against load-cell outputs. Modified to acquire strain data for other specimens wherever full-bridge strain-gauge circuits used. Written in HP BASIC.

  16. Mesoscale hybrid calibration artifact

    Science.gov (United States)

    Tran, Hy D.; Claudet, Andre A.; Oliver, Andrew D.

    2010-09-07

    A mesoscale calibration artifact, also called a hybrid artifact, suitable for hybrid dimensional measurement and the method for make the artifact. The hybrid artifact has structural characteristics that make it suitable for dimensional measurement in both vision-based systems and touch-probe-based systems. The hybrid artifact employs the intersection of bulk-micromachined planes to fabricate edges that are sharp to the nanometer level and intersecting planes with crystal-lattice-defined angles.

  17. Dosimetry and Calibration Section

    International Nuclear Information System (INIS)

    The two tasks of the Dosimetry and Calibration Section at CERN are the Individual Dosimetry Service which assures the personal monitoring of about 5000 persons potentially exposed to ionizing radiation at CERN, and the Calibration Laboratory which verifies all the instruments and monitors. This equipment is used by the sections of the RP Group for assuring radiation protection around CERN's accelerators, and by the Environmental Section of TISTE. In addition, nearly 250 electronic and 300 quartz fibre dosimeters, employed in operational dosimetry, are calibrated at least once a year. The Individual Dosimetry Service uses an extended database (INDOS) which contains information about all the individual doses ever received at CERN. For most of 1997 it was operated without the support of a database administrator as the technician who had assured this work retired. The Software Support Section of TIS-TE took over the technical responsibility of the database, but in view of the many other tasks of this Section and the lack of personnel, only a few interventions for solving immediate problems were possible

  18. Calibration of Underwater Sound Transducers

    Directory of Open Access Journals (Sweden)

    H.R.S. Sastry

    1983-07-01

    Full Text Available The techniques of calibration of underwater sound transducers for farfield, near-field and closed environment conditions are reviewed in this paper .The design of acoustic calibration tank is mentioned. The facilities available at Naval Physical & Oceanographic Laboratory, Cochin for calibration of transducers are also listed.

  19. Radioreceptor opioid assay

    International Nuclear Information System (INIS)

    A radioreceptor assay is described for assaying opioid drugs in biological fluids. The method enables the assay of total opioid activity, being specific for opioids as a class but lacking specificity within the class. A radio-iodinated opioid and the liquid test sample are incubated with an opiate receptor material. The percentage inhibition of the binding of the radio-iodinated compound to the opiate receptor is calculated and the opioid activity of the test liquid determined from a standard curve. Examples of preparing radio-iodinated opioids and assaying opioid activity are given. A test kit for the assay is described. Compared to other methods, this assay is cheap, easy and rapid. (U.K.)

  20. Absolute nuclear material assay

    Science.gov (United States)

    Prasad, Manoj K.; Snyderman, Neal J.; Rowland, Mark S.

    2010-07-13

    A method of absolute nuclear material assay of an unknown source comprising counting neutrons from the unknown source and providing an absolute nuclear material assay utilizing a model to optimally compare to the measured count distributions. In one embodiment, the step of providing an absolute nuclear material assay comprises utilizing a random sampling of analytically computed fission chain distributions to generate a continuous time-evolving sequence of event-counts by spreading the fission chain distribution in time.

  1. Identity, Diversity and Diversity Management

    DEFF Research Database (Denmark)

    Holck, Lotte; Muhr, Sara Louise; Villeseche, Florence

    2016-01-01

    The purpose of this paper is to examine the relationship between the identity and diversity literatures and discuss how a better understanding of the theoretical connections between the two informs both diversity research and diversity management practices. Design/methodology/approach – Literature...... review followed by a discussion of the theoretical and practical consequences of connecting the identity and diversity literatures. Findings – The authors inform future research in three ways. First, by showing how definitions of identity influence diversity theorizing in specific ways. Second......, the authors explore how such definitions entail distinct foci regarding how diversity should be analyzed and interventions actioned. Third, the authors discuss how theoretical coherence between definitions of identity and diversity perspectives – as well as knowledge about a perspective’s advantages...

  2. Calibration effects on orbit determination

    Science.gov (United States)

    Madrid, G. A.; Winn, F. B.; Zielenbach, J. W.; Yip, K. B.

    1974-01-01

    The effects of charged particle and tropospheric calibrations on the orbit determination (OD) process are analyzed. The calibration process consisted of correcting the Doppler observables for the media effects. Calibrated and uncalibrated Doppler data sets were used to obtain OD results for past missions as well as Mariner Mars 1971. Comparisons of these Doppler reductions show the significance of the calibrations. For the MM'71 mission, the media calibrations proved themselves effective in diminishing the overall B-plane error and reducing the Doppler residual signatures.

  3. A variable acceleration calibration system

    Science.gov (United States)

    Johnson, Thomas H.

    2011-12-01

    A variable acceleration calibration system that applies loads using gravitational and centripetal acceleration serves as an alternative, efficient and cost effective method for calibrating internal wind tunnel force balances. Two proof-of-concept variable acceleration calibration systems are designed, fabricated and tested. The NASA UT-36 force balance served as the test balance for the calibration experiments. The variable acceleration calibration systems are shown to be capable of performing three component calibration experiments with an approximate applied load error on the order of 1% of the full scale calibration loads. Sources of error are indentified using experimental design methods and a propagation of uncertainty analysis. Three types of uncertainty are indentified for the systems and are attributed to prediction error, calibration error and pure error. Angular velocity uncertainty is shown to be the largest indentified source of prediction error. The calibration uncertainties using a production variable acceleration based system are shown to be potentially equivalent to current methods. The production quality system can be realized using lighter materials and a more precise instrumentation. Further research is needed to account for balance deflection, forcing effects due to vibration, and large tare loads. A gyroscope measurement technique is shown to be capable of resolving the balance deflection angle calculation. Long term research objectives include a demonstration of a six degree of freedom calibration, and a large capacity balance calibration.

  4. Cultural diversity

    OpenAIRE

    Raghavan, Raghu

    2011-01-01

    The concept of cultural diversity has emerged as an influential one having impact on multiple policy and legal instruments especially following the adoption of the UNESCO Convention on the Protection and Promotion of the Diversity of Cultural Expressions in 2005. The discussions on its appropriate implementation are however profoundly fragmented and often laden with political considerations. The present brief paper offers some thoughts on the meaning of cultural diversity and its implementati...

  5. SAR antenna calibration techniques

    Science.gov (United States)

    Carver, K. R.; Newell, A. C.

    1978-01-01

    Calibration of SAR antennas requires a measurement of gain, elevation and azimuth pattern shape, boresight error, cross-polarization levels, and phase vs. angle and frequency. For spaceborne SAR antennas of SEASAT size operating at C-band or higher, some of these measurements can become extremely difficult using conventional far-field antenna test ranges. Near-field scanning techniques offer an alternative approach and for C-band or X-band SARs, give much improved accuracy and precision as compared to that obtainable with a far-field approach.

  6. Use of Radiometrically Calibrated Flat-Plate Calibrators in Calibration of Radiation Thermometers

    Science.gov (United States)

    Cárdenas-García, D.; Méndez-Lango, E.

    2015-08-01

    Most commonly used, low-temperature, infrared thermometers have large fields of view sizes that make them difficult to be calibrated with narrow aperture blackbodies. Flat-plate calibrators with large emitting surfaces have been proposed for calibrating these infrared thermometers. Because the emissivity of the flat plate is not unity, its radiance temperature is wavelength dependent. For calibration, the wavelength pass band of the device under test should match that of the reference infrared thermometer. If the device under test and reference radiometer have different pass bands, then it is possible to calculate the corresponding correction if the emissivity of the flat plate is known. For example, a correction of at is required when calibrating a infrared thermometer with a "" radiometrically calibrated flat-plate calibrator. A method is described for using a radiometrically calibrated flat-plate calibrator that covers both cases of match and mismatch working wavelength ranges of a reference infrared thermometer and infrared thermometers to be calibrated with the flat-plate calibrator. Also, an application example is included in this paper.

  7. A Simple Accelerometer Calibrator

    Science.gov (United States)

    Salam, R. A.; Islamy, M. R. F.; Munir, M. M.; Latief, H.; Irsyam, M.; Khairurrijal

    2016-08-01

    High possibility of earthquake could lead to the high number of victims caused by it. It also can cause other hazards such as tsunami, landslide, etc. In that case it requires a system that can examine the earthquake occurrence. Some possible system to detect earthquake is by creating a vibration sensor system using accelerometer. However, the output of the system is usually put in the form of acceleration data. Therefore, a calibrator system for accelerometer to sense the vibration is needed. In this study, a simple accelerometer calibrator has been developed using 12 V DC motor, optocoupler, Liquid Crystal Display (LCD) and AVR 328 microcontroller as controller system. The system uses the Pulse Wave Modulation (PWM) form microcontroller to control the motor rotational speed as response to vibration frequency. The frequency of vibration was read by optocoupler and then those data was used as feedback to the system. The results show that the systems could control the rotational speed and the vibration frequencies in accordance with the defined PWM.

  8. On chromatic and geometrical calibration

    DEFF Research Database (Denmark)

    Folm-Hansen, Jørgen

    1999-01-01

    we present the implementation of a complete calibration method for an accurate colour texture measurement device called VMX2000, the calibration for uneven laser sheet illumination in a flow measuring system and the use of automatic detection of calibration targets for a DLT/warping in a 3D PIV......The main subject of the present thesis is different methods for the geometrical and chromatic calibration of cameras in various environments. For the monochromatic issues of the calibration we present the acquisition of monochrome images, the classic monochrome aberrations and the various sources...... of non-uniformity of the illumination of the image plane. Only the image deforming aberrations and the non-uniformity of illumination are included in the calibration models. The topics of the pinhole camera model and the extension to the Direct Linear Transform (DLT) are described. It is shown how...

  9. Optimising automation of a manual enzyme-linked immunosorbent assay

    Directory of Open Access Journals (Sweden)

    Corena de Beer

    2011-12-01

    Full Text Available Objective: Enzyme-linked immunosorbent assays (ELISAs are widely used to quantify immunoglobulin levels induced by infection or vaccination. Compared to conventional manual assays, automated ELISA systems offer more accurate and reproducible results, faster turnaround times and cost effectiveness due to the use of multianalyte reagents.Design: The VaccZyme™ Human Anti-Haemophilus influenzae type B (Hib kit (MK016 from The Binding Site Company was optimised to be used on an automated BioRad PhD™ system in the Immunology Laboratory (National Health Laboratory Service in Tygerberg, South Africa.Methods: An automated ELISA system that uses individual well incubation was compared to a manual method that uses whole-plate incubation.Results: Results were calculated from calibration curves constructed with each assay. Marked differences in calibration curves were observed for the two methods. The automated method produced lower-than-recommended optical density values and resulted in invalid calibration curves and diagnostic results. A comparison of the individual steps of the two methods showed a difference of 10 minutes per incubation cycle. All incubation steps of the automated method were subsequently increased from 30 minutes to 40 minutes. Several comparative assays were performed according to the amended protocol and all calibration curves obtained were valid. Calibrators and controls were also included as samples in different positions and orders on the plate and all results were valid.Conclusion: Proper validation is vital before converting manual ELISA assays to automated or semi-automated methods. 

  10. Calibration procedure for zenith plummets

    Directory of Open Access Journals (Sweden)

    Jelena GUČEVIĆ

    2013-09-01

    Full Text Available Zenith plummets are used mainly in applied geodesy, in civil engineering surveying, for materialization of the local vertical. The error of the vertical deflection of the instrument is directly transferred to the error of the observing construction. That is why a proper calibration procedure for the zenithlot is required. Metrological laboratory of the Faculty of Civil Engineering in Belgrade developed such calibration procedure. Here we present a mathematical model of the calibration and some selected results.

  11. Calibration procedure for zenith plummets

    OpenAIRE

    Jelena GUČEVIĆ; Delčev, Siniša; Vukan OGRIZOVIĆ

    2013-01-01

    Zenith plummets are used mainly in applied geodesy, in civil engineering surveying, for materialization of the local vertical. The error of the vertical deflection of the instrument is directly transferred to the error of the observing construction. That is why a proper calibration procedure for the zenithlot is required. Metrological laboratory of the Faculty of Civil Engineering in Belgrade developed such calibration procedure. Here we present a mathematical model of the calibration and som...

  12. Calibration of neutron albedo dosemeters.

    Science.gov (United States)

    Schwartz, R B; Eisenhauer, C M

    2002-01-01

    It is shown that by calibrating neutron albedo dosemeters under the proper conditions, two complicating effects will essentially cancel out, allowing accurate calibrations with no need for explicit corrections. The 'proper conditions' are: a large room (> or = 8 m on a side). use of a D2O moderated 252Cf source, and a source-to-phantom calibration distance of approximately 70 cm. PMID:12212898

  13. CPTAC Assay Portal: a repository of targeted proteomic assays

    Energy Technology Data Exchange (ETDEWEB)

    Whiteaker, Jeffrey R.; Halusa, Goran; Hoofnagle, Andrew N.; Sharma, Vagisha; MacLean, Brendan; Yan, Ping; Wrobel, John; Kennedy, Jacob; Mani, DR; Zimmerman, Lisa J.; Meyer, Matthew R.; Mesri, Mehdi; Rodriguez, Henry; Abbateillo, Susan E.; Boja, Emily; Carr, Steven A.; Chan, Daniel W.; Chen, Xian; Chen, Jing; Davies, Sherri; Ellis, Matthew; Fenyo, David; Hiltket, Tara; Ketchum, Karen; Kinsinger, Christopher; Kuhn, Eric; Liebler, Daniel; Lin, De; Liu, Tao; Loss, Michael; MacCoss, Michael; Qian, Weijun; Rivers, Robert; Rodland, Karin D.; Ruggles, Kelly; Scott, Mitchell; Smith, Richard D.; Thomas, Stefani N.; Townsend, Reid; Whiteley, Gordon; Wu, Chaochao; Zhang, Hui; Zhang, Zhen; Paulovich, Amanda G.

    2014-06-27

    To address these issues, the Clinical Proteomic Tumor Analysis Consortium (CPTAC) of the National Cancer Institute (NCI) has launched an Assay Portal (http://assays.cancer.gov) to serve as a public repository of well-characterized quantitative, MS-based, targeted proteomic assays. The purpose of the CPTAC Assay Portal is to facilitate widespread adoption of targeted MS assays by disseminating SOPs, reagents, and assay characterization data for highly characterized assays. A primary aim of the NCI-supported portal is to bring together clinicians or biologists and analytical chemists to answer hypothesis-driven questions using targeted, MS-based assays. Assay content is easily accessed through queries and filters, enabling investigators to find assays to proteins relevant to their areas of interest. Detailed characterization data are available for each assay, enabling researchers to evaluate assay performance prior to launching the assay in their own laboratory.

  14. Radiological Calibration and Standards Facility

    Data.gov (United States)

    Federal Laboratory Consortium — PNNL maintains a state-of-the-art Radiological Calibration and Standards Laboratory on the Hanford Site at Richland, Washington. Laboratory staff provide expertise...

  15. Calibration Techniques for VERITAS

    CERN Document Server

    Hanna, David

    2007-01-01

    VERITAS is an array of four identical telescopes designed for detecting and measuring astrophysical gamma rays with energies in excess of 100 GeV. Each telescope uses a 12 m diameter reflector to collect Cherenkov light from air showers initiated by incident gamma rays and direct it onto a `camera' comprising 499 photomultiplier tubes read out by flash ADCs. We describe here calibration methods used for determining the values of the parameters which are necessary for converting the digitized PMT pulses to gamma-ray energies and directions. Use of laser pulses to determine and monitor PMT gains is discussed, as are measurements of the absolute throughput of the telescopes using muon rings.

  16. RX130 Robot Calibration

    Science.gov (United States)

    Fugal, Mario

    2012-10-01

    In order to create precision magnets for an experiment at Oak Ridge National Laboratory, a new reverse engineering method has been proposed that uses the magnetic scalar potential to solve for the currents necessary to produce the desired field. To make the magnet it is proposed to use a copper coated G10 form, upon which a drill, mounted on a robotic arm, will carve wires. The accuracy required in the manufacturing of the wires exceeds nominal robot capabilities. However, due to the rigidity as well as the precision servo motor and harmonic gear drivers, there are robots capable of meeting this requirement with proper calibration. Improving the accuracy of an RX130 to be within 35 microns (the accuracy necessary of the wires) is the goal of this project. Using feedback from a displacement sensor, or camera and inverse kinematics it is possible to achieve this accuracy.

  17. TOD to TTP calibration

    Science.gov (United States)

    Bijl, Piet; Reynolds, Joseph P.; Vos, Wouter K.; Hogervorst, Maarten A.; Fanning, Jonathan D.

    2011-05-01

    The TTP (Targeting Task Performance) metric, developed at NVESD, is the current standard US Army model to predict EO/IR Target Acquisition performance. This model however does not have a corresponding lab or field test to empirically assess the performance of a camera system. The TOD (Triangle Orientation Discrimination) method, developed at TNO in The Netherlands, provides such a measurement. In this study, we make a direct comparison between TOD performance for a range of sensors and the extensive historical US observer performance database built to develop and calibrate the TTP metric. The US perception data were collected doing an identification task by military personnel on a standard 12 target, 12 aspect tactical vehicle image set that was processed through simulated sensors for which the most fundamental sensor parameters such as blur, sampling, spatial and temporal noise were varied. In the present study, we measured TOD sensor performance using exactly the same sensors processing a set of TOD triangle test patterns. The study shows that good overall agreement is obtained when the ratio between target characteristic size and TOD test pattern size at threshold equals 6.3. Note that this number is purely based on empirical data without any intermediate modeling. The calibration of the TOD to the TTP is highly beneficial to the sensor modeling and testing community for a variety of reasons. These include: i) a connection between requirement specification and acceptance testing, and ii) a very efficient method to quickly validate or extend the TTP range prediction model to new systems and tasks.

  18. Calibration of Nanopositioning Stages

    Directory of Open Access Journals (Sweden)

    Ning Tan

    2015-12-01

    Full Text Available Accuracy is one of the most important criteria for the performance evaluation of micro- and nanorobots or systems. Nanopositioning stages are used to achieve the high positioning resolution and accuracy for a wide and growing scope of applications. However, their positioning accuracy and repeatability are not well known and difficult to guarantee, which induces many drawbacks for many applications. For example, in the mechanical characterisation of biological samples, it is difficult to perform several cycles in a repeatable way so as not to induce negative influences on the study. It also prevents one from controlling accurately a tool with respect to a sample without adding additional sensors for closed loop control. This paper aims at quantifying the positioning repeatability and accuracy based on the ISO 9283:1998 standard, and analyzing factors influencing positioning accuracy onto a case study of 1-DoF (Degree-of-Freedom nanopositioning stage. The influence of thermal drift is notably quantified. Performances improvement of the nanopositioning stage are then investigated through robot calibration (i.e., open-loop approach. Two models (static and adaptive models are proposed to compensate for both geometric errors and thermal drift. Validation experiments are conducted over a long period (several days showing that the accuracy of the stage is improved from typical micrometer range to 400 nm using the static model and even down to 100 nm using the adaptive model. In addition, we extend the 1-DoF calibration to multi-DoF with a case study of a 2-DoF nanopositioning robot. Results demonstrate that the model efficiently improved the 2D accuracy from 1400 nm to 200 nm.

  19. Transgenic Animal Mutation Assays

    Institute of Scientific and Technical Information of China (English)

    Tao Chen; Ph.D.D.A.B.T.

    2005-01-01

    @@ The novel transgenic mouse and rat mutation assays have provided a tool for analyzing in vivo mutation in any tissue, thus permitting the direct comparison of cancer incidence with mutant frequency.

  20. Lateral flow assays.

    Science.gov (United States)

    Koczula, Katarzyna M; Gallotta, Andrea

    2016-06-30

    Lateral flow assays (LFAs) are the technology behind low-cost, simple, rapid and portable detection devices popular in biomedicine, agriculture, food and environmental sciences. This review presents an overview of the principle of the method and the critical components of the assay, focusing on lateral flow immunoassays. This type of assay has recently attracted considerable interest because of its potential to provide instantaneous diagnosis directly to patients. The range and interpretation of results and parameters used for evaluation of the assay will also be discussed. The main advantages and disadvantages of LFAs will be summarized and relevant future improvements to testing devices and strategies will be proposed. Finally, the major recent advances and future diagnostic applications in the LFA field will be explored. PMID:27365041

  1. Nondestructive assay instrumentation for Savannah River Plant reprocessing accountability

    International Nuclear Information System (INIS)

    We have designed, developed, and calibrated three different types of nondestructive assay systems for the Savannah River Plant (SRP). These systems will be delivered to SRP in 1986 and become part of the nuclear material accounting instrumentation at one of SRP's reprocessing facilities. Among the various types of nondestructive assay systems to be implemented are a neutron counter (Los Alamos National Laboratory - LANL), a four-station calorimeter (Mound Laboratories), a waste solution assay system (LANL), two gamma-ray solution concentration assay systems (LLNL), two x-ray fluorescence analysis concentration assay systems (LLNL), and one 2-detector plutonium solids isotopics system (LLNL). Los Alamos also has the responsibility of combining the individual measurement systems into an integrated accountability capability. Each NDA instrument will report results to a central Instrument Control Computer (ICC). Figure 1 illustrates schematically the integrated system with each Laboratory's contribution shown by dotted lines

  2. New Rapid Spore Assay

    Science.gov (United States)

    Kminek, Gerhard; Conley, Catharine

    2012-07-01

    The presentation will detail approved Planetary Protection specifications for the Rapid Spore Assay for spacecraft components and subsystems. Outlined will be the research and studies on which the specifications were based. The research, funded by ESA and NASA/JPL, was conducted over a period of two years and was followed by limited cleanroom studies to assess the feasibility of this assay during spacecraft assembly.

  3. Understanding Diversity

    NARCIS (Netherlands)

    D.L. van Knippenberg (Daan)

    2007-01-01

    textabstractDaan van Knippenberg is Professor of Organizational Behavior at RSM Erasmus University, Erasmus University Rotterdam, The Netherlands. His research interests include work group performance, especially work group diversity and group decision making, leadership, in particular the roles of

  4. Gender Diversities

    DEFF Research Database (Denmark)

    Agustin, Lise Rolandsen; Siim, Birte

    2014-01-01

    The article analyses the European Year for Combating Poverty and Social Exclusion (2010) (EY 2010) with the aim of identifying the nature of gender diversities in EU policies. We argue that the EU handles issues related to gender and diversity in particular ways; this approach is characterized...... by non-citizen/citizen and redistribution/recognition divisions. Employing intersectionality as the methodological approach to gender diversities, the article shows how gender and ethnicity are articulated in the policy-making process which led to the adoption of EY 201, the activities undertaken during...... the EY 2010, and the evaluation of EY 2010. The case study is suitable for developing a dynamic multi-level model for analysing gendered diversities at the transnationmal level: It illustrates how the EU policy frame interacts with particular national contexts in promoting or hundering the advancement...

  5. Diversity management

    OpenAIRE

    Kupková, Barbora

    2014-01-01

    The Bachelor's Thesis analyses the extent of integration of Diversity Management in selected companies from the Czech Republic as well as application of its methods during a recruitment process. The thesis aims to map out the role of particular HR departments in this field of management and also to find out whether those companies analyse or evaluate the results and benefits of this modern approach. The attitudes to managing diversity of selected companies and the tools they uses for that wer...

  6. Challenges in X-band Weather Radar Data Calibration

    DEFF Research Database (Denmark)

    Thorndahl, Søren; Rasmussen, Michael R.

    2009-01-01

    Application of weather radar data in urban hydrology is evolving and radar data is now applied for both modelling, analysis and real time control purposes. In these contexts, it is all-important that the radar data well calibrated and adjusted in order to obtain valid quantitative precipitation...... estimates. This paper compares two calibration procedures for a small marine X-band radar by comparing radar data with rain gauge data. Validation shows a very good consensus with regards to precipitation volumes, but more diverse results on peak rain intensities....

  7. Tectonic calibrations in molecular dating

    Institute of Scientific and Technical Information of China (English)

    Ullasa KODANDARAMAIAH

    2011-01-01

    Molecular dating techniques require the use of calibrations, which are usually fossil or geological vicariance-based.Fossil calibrations have been criticised because they result only in minimum age estimates. Based on a historical biogeographic perspective, Ⅰ suggest that vicariance-based calibrations are more dangerous. Almost all analytical methods in historical biogeography are strongly biased towards inferring vicariance, hence vicariance identified through such methods is unreliable. Other studies, especially of groups found on Gondwanan fragments, have simply assumed vicariance. Although it was previously believed that vicariance was the predominant mode of speciation, mounting evidence now indicates that speciation by dispersal is common, dominating vicariance in several groups. Moreover, the possibility of speciation having occurred before the said geological event cannot be precluded. Thus, geological calibrations can under- or overestimate times, whereas fossil calibrations always result in minimum estimates. Another major drawback of vicariant calibrations is the problem of circular reasoning when the resulting estimates are used to infer ages of biogeographic events. Ⅰ argue that fossil-based dating is a superior alternative to vicariance, primarily because the strongest assumption in the latter, that speciation was caused by the said geological process, is more often than not the most tenuous. When authors prefer to use a combination of fossil and vicariant calibrations, one suggestion is to report results both with and without inclusion of the geological constraints. Relying solely on vicariant calibrations should be strictly avoided.

  8. The Advanced LIGO Photon Calibrators

    CERN Document Server

    Karki, S; Kandhasamy, S; Abbott, B P; Abbott, T D; Anders, E H; Berliner, J; Betzwieser, J; Daveloza, H P; Cahillane, C; Canete, L; Conley, C; Gleason, J R; Goetz, E; Kissel, J S; Izumi, K; Mendell, G; Quetschke, V; Rodruck, M; Sachdev, S; Sadecki, T; Schwinberg, P B; Sottile, A; Wade, M; Weinstein, A J; West, M; Savage, R L

    2016-01-01

    The two interferometers of the Laser Interferometry Gravitaional-wave Observatory (LIGO) recently detected gravitational waves from the mergers of binary black hole systems. Accurate calibration of the output of these detectors was crucial for the observation of these events, and the extraction of parameters of the sources. The principal tools used to calibrate the responses of the second-generation (Advanced) LIGO detectors to gravitational waves are systems based on radiation pressure and referred to as Photon Calibrators. These systems, which were completely redesigned for Advanced LIGO, include several significant upgrades that enable them to meet the calibration requirements of second-generation gravitational wave detectors in the new era of gravitational-wave astronomy. We report on the design, implementation, and operation of these Advanced LIGO Photon Calibrators that are currently providing fiducial displacements on the order of $10^{-18}$ m/$\\sqrt{\\textrm{Hz}}$ with accuracy and precision of better ...

  9. Antenna Calibration and Measurement Equipment

    Science.gov (United States)

    Rochblatt, David J.; Cortes, Manuel Vazquez

    2012-01-01

    A document describes the Antenna Calibration & Measurement Equipment (ACME) system that will provide the Deep Space Network (DSN) with instrumentation enabling a trained RF engineer at each complex to perform antenna calibration measurements and to generate antenna calibration data. This data includes continuous-scan auto-bore-based data acquisition with all-sky data gathering in support of 4th order pointing model generation requirements. Other data includes antenna subreflector focus, system noise temperature and tipping curves, antenna efficiency, reports system linearity, and instrument calibration. The ACME system design is based on the on-the-fly (OTF) mapping technique and architecture. ACME has contributed to the improved RF performance of the DSN by approximately a factor of two. It improved the pointing performances of the DSN antennas and productivity of its personnel and calibration engineers.

  10. Design, construction, and validation of a modular library of sequence diversity standards for polymerase chain reaction.

    Science.gov (United States)

    Baum, Paul D; Young, Jennifer J; Zhang, Qianjun; Kasakow, Zeljka; McCune, Joseph M

    2011-04-01

    Methods to measure the sequence diversity of polymerase chain reaction (PCR)-amplified DNA lack standards for use as assay calibrators and controls. Here we present a general and economical method for developing customizable DNA standards of known sequence diversity. Standards ranging from 1 to 25,000 sequences were generated by directional ligation of oligonucleotide "words" of standard length and GC content and then amplified by PCR. The sequence accuracy and diversity of the library were validated using AmpliCot analysis (DNA hybridization kinetics) and Illumina sequencing. The library has the following features: (i) pools containing tens of thousands of sequences can be generated from the ligation of relatively few commercially synthesized short oligonucleotides; (ii) each sequence differs from all others in the library at a minimum of three nucleotide positions, permitting discrimination between different sequences by either sequencing or hybridization; (iii) all sequences have identical length, GC content, and melting temperature; (iv) the identity of each standard can be verified by restriction digestion; and (v) once made, the ends of the library may be cleaved and replaced with sequences to match any PCR primer pair. These standards should greatly improve the accuracy and reproducibility of sequence diversity measurements.

  11. Computer-determined assay time based on preset precision

    Energy Technology Data Exchange (ETDEWEB)

    Foster, L.A.; Hagan, R.; Martin, E.R.; Wachter, J.R.; Bonner, C.A.; Malcom, J.E. [Los Alamos National Lab., NM (United States). Nuclear Materials Measurement and Accountability

    1994-08-01

    Most current assay systems for special nuclear materials (SNM) operate on the principle of a fixed assay time which provides acceptable measurement precision without sacrificing the required throughput of the instrument. Waste items to be assayed for SNM content can contain a wide range of nuclear material. Counting all items for the same preset assay time results in a wide range of measurement precision and wastes time at the upper end of the calibration range. A short time sample taken at the beginning of the assay could optimize the analysis time on the basis of the required measurement precision. To illustrate the technique of automatically determining the assay time, measurements were made with a segmented gamma scanner at the Plutonium Facility of Los Alamos National Laboratory with the assay time for each segment determined by counting statistics in that segment. Segments with very little SNM were quickly determined to be below the lower limit of the measurement range and the measurement was stopped. Segments with significant SNM were optimally assays to the preset precision. With this method the total assay time for each item is determined by the desired preset precision. This report describes the precision-based algorithm and presents the results of measurements made to test its validity.

  12. Assay method and compositions

    International Nuclear Information System (INIS)

    Methods are described for measuring catecholamine levels in human and animal body fluids and tissues using the catechol-O-methyl-transferase (COMT) radioassay. The assay involves incubating the biological sample with COMT and the tritiated methyl donor, S-adenosyl-L-methionine(3H)-methyl. The O-methylated (3H) epinephrine and/or norepinephrine are extracted and oxidised to vanillin-3H which in turn is extracted and its radioactivity counted. When analysing dopamine levels the assay is extended by vanillin-3H and raising the pH of the aqueous periodate phase from which O-methylated (3H) dopamine is extracted and counted. The assay may be modified depending on whether measurements of undifferentiated total endogenous catecholamine levels or differential analyses of the catecholamine levels are being performed. The sensitivity of the assay can be as low as 5 picograms for norepinephrine and epinephrine and 12 picograms for dopamine. The assemblance of the essential components of the assay into a kit for use in laboratories is also described. (U.K.)

  13. A calibrated Franklin chimes

    Science.gov (United States)

    Gonta, Igor; Williams, Earle

    1994-05-01

    Benjamin Franklin devised a simple yet intriguing device to measure electrification in the atmosphere during conditions of foul weather. He constructed a system of bells, one of which was attached to a conductor that was suspended vertically above his house. The device is illustrated in a well-known painting of Franklin (Cohen, 1985). The elevated conductor acquired a potential due to the electric field in the atmosphere and caused a brass ball to oscillate between two bells. The purpose of this study is to extend Franklin's idea by constructing a set of 'chimes' which will operate both in fair and in foul weather conditions. In addition, a mathematical relationship will be established between the frequency of oscillation of a metallic sphere in a simplified geometry and the potential on one plate due to the electrification of the atmosphere. Thus it will be possible to calibrate the 'Franklin Chimes' and to obtain a nearly instantaneous measurement of the potential of the elevated conductor in both fair and foul weather conditions.

  14. Automated assay data processing and quality control: A review and recommendations

    International Nuclear Information System (INIS)

    Automated data processing and quality control of assays offers not only increased speed but also a more thorough and statistically rigorous analysis of results. This review outlines the motivations, statistical definitions, and mathematical methods pertinent to assay data processing. The presentation concentrates on basic concepts rather than specific mathematical formulae. The numerous automated calibration procedures are discussed and summarized in tabular form. A comprehensive view of data processing is offered which includes much more than simple calibration and interpolation. A small number of calculator and computer programs which provide an acceptably detailed statistical analysis of assays are recommended. Finally, possible future developments in hardware and software are discussed. (author)

  15. Interpreting coagulation assays.

    Science.gov (United States)

    Green, David

    2010-09-01

    The interpretation of coagulation assays requires knowledge of the principal clotting pathways. The activated partial thromboplastin time is sensitive to all hemostatic factors except FVII, whereas the prothrombin time reflects levels of prothrombin and FV, FVII, and FX. Using the two tests in concert is helpful in identifying hemophilia, the coagulopathy of liver disease, and disseminated intravascular coagulation. In addition, the activated partial thromboplastin time and prothrombin time are used for monitoring anticoagulant therapy with heparin and warfarin, respectively. Measurement of D-dimer is informative in patients suspected of having thrombotic disorders and determining the risk of thrombosis recurrence. Mixing tests distinguish clotting factor deficiencies from circulating anticoagulants such as heparin, the lupus anticoagulant, and antibodies directed against specific clotting factors. The modified Bethesda assay detects and provides an indication of the strength of FVIII inhibitors. However, interpreting the results of coagulation assays is not always straightforward, and expert consultation is occasionally required to resolve difficult clinical situations. PMID:20855988

  16. Mercury Continuous Emmission Monitor Calibration

    Energy Technology Data Exchange (ETDEWEB)

    John Schabron; Eric Kalberer; Ryan Boysen; William Schuster; Joseph Rovani

    2009-03-12

    Mercury continuous emissions monitoring systems (CEMs) are being implemented in over 800 coal-fired power plant stacks throughput the U.S. Western Research Institute (WRI) is working closely with the Electric Power Research Institute (EPRI), the National Institute of Standards and Technology (NIST), and the Environmental Protection Agency (EPA) to facilitate the development of the experimental criteria for a NIST traceability protocol for dynamic elemental mercury vapor calibrators/generators. These devices are used to calibrate mercury CEMs at power plant sites. The Clean Air Mercury Rule (CAMR) which was published in the Federal Register on May 18, 2005 and vacated by a Federal appeals court in early 2008 required that calibration be performed with NIST-traceable standards. Despite the vacature, mercury emissions regulations in the future will require NIST traceable calibration standards, and EPA does not want to interrupt the effort towards developing NIST traceability protocols. The traceability procedures will be defined by EPA. An initial draft traceability protocol was issued by EPA in May 2007 for comment. In August 2007, EPA issued a conceptual interim traceability protocol for elemental mercury calibrators. The protocol is based on the actual analysis of the output of each calibration unit at several concentration levels ranging initially from about 2-40 {micro}g/m{sup 3} elemental mercury, and in the future down to 0.2 {micro}g/m{sup 3}, and this analysis will be directly traceable to analyses by NIST. The EPA traceability protocol document is divided into two separate sections. The first deals with the qualification of calibrator models by the vendors for use in mercury CEM calibration. The second describes the procedure that the vendors must use to certify the calibrators that meet the qualification specifications. The NIST traceable certification is performance based, traceable to analysis using isotope dilution inductively coupled plasma

  17. Neutral Comet Assay

    OpenAIRE

    2013-01-01

    The Comet assay (or Single Cell Gel Electrophoresis assay) is a sensitive technique to detect DNA damage at the level of an individual cell. This technique is based on micro-electrophoresis of cells DNA content. Briefly, cells are embedded in agarose, lysed and submitted to an electric field, before the staining step with a fluorescent DNA binding dye. Damaged DNA (charged DNA) migrates in this field, forming the tail of a “comet”, while undamaged DNA remained in the head of the “comet”. The ...

  18. Lateral flow strip assay

    Science.gov (United States)

    Miles, Robin R.; Benett, William J.; Coleman, Matthew A.; Pearson, Francesca S.; Nasarabadi, Shanavaz L.

    2011-03-08

    A lateral flow strip assay apparatus comprising a housing; a lateral flow strip in the housing, the lateral flow strip having a receiving portion; a sample collection unit; and a reagent reservoir. Saliva and/or buccal cells are collected from an individual using the sample collection unit. The sample collection unit is immersed in the reagent reservoir. The tip of the lateral flow strip is immersed in the reservoir and the reagent/sample mixture wicks up into the lateral flow strip to perform the assay.

  19. Automated phantom assay system

    International Nuclear Information System (INIS)

    This paper describes an automated phantom assay system developed for assaying phantoms spiked with minute quantities of radionuclides. The system includes a computer-controlled linear-translation table that positions the phantom at exact distances from a spectrometer. A multichannel analyzer (MCA) interfaces with a computer to collect gamma spectral data. Signals transmitted between the controller and MCA synchronize data collection and phantom positioning. Measured data are then stored on disk for subsequent analysis. The automated system allows continuous unattended operation and ensures reproducible results

  20. A comprehensive company database analysis of biological assay variability.

    Science.gov (United States)

    Kramer, Christian; Dahl, Göran; Tyrchan, Christian; Ulander, Johan

    2016-08-01

    Analysis of data from various compounds measured in diverse biological assays is a central part of drug discovery research projects. However, no systematic overview of the variability in biological assays has been published and judgments on assay quality and robustness of data are often based on personal belief and experience within the drug discovery community. To address this we performed a reproducibility analysis of all biological assays at AstraZeneca between 2005 and 2014. We found an average experimental uncertainty of less than a twofold difference and no technologies or assay types had higher variability than others. This work suggests that robust data can be obtained from the most commonly applied biological assays.

  1. Mexican national pyronometer network calibration

    Science.gov (United States)

    VAldes, M.; Villarreal, L.; Estevez, H.; Riveros, D.

    2013-12-01

    In order to take advantage of the solar radiation as an alternate energy source it is necessary to evaluate the spatial and temporal availability. The Mexican National Meterological Service (SMN) has a network with 136 meteorological stations, each coupled with a pyronometer for measuring the global solar radiation. Some of these stations had not been calibrated in several years. The Mexican Department of Energy (SENER) in order to count on a reliable evaluation of the solar resource funded this project to calibrate the SMN pyrometer network and validate the data. The calibration of the 136 pyronometers by the intercomparison method recommended by the World Meterological Organization (WMO) requires lengthy observations and specific environmental conditions such as clear skies and a stable atmosphere, circumstances that determine the site and season of the calibration. The Solar Radiation Section of the Instituto de Geofísica of the Universidad Nacional Autónoma de México is a Regional Center of the WMO and is certified to carry out the calibration procedures and emit certificates. We are responsible for the recalibration of the pyronometer network of the SMN. A continuous emission solar simulator with exposed areas with 30cm diameters was acquired to reduce the calibration time and not depend on atmospheric conditions. We present the results of the calibration of 10 thermopile pyronometers and one photovoltaic cell by the intercomparison method with more than 10000 observations each and those obtained with the solar simulator.

  2. Lateral flow assays

    NARCIS (Netherlands)

    Posthuma-Trumpie, G.A.; Amerongen, van A.

    2012-01-01

    A simple version of immunochemical-based methods is the Lateral Flow Assay (LFA). It is a dry chemistry technique (reagents are included); the fluid from the sample runs through a porous membrane (often nitrocellulose) by capillary force. Typically the membrane is cut as a strip of 0.5*5 cm. In most

  3. Hyaluronic Acid Assays

    DEFF Research Database (Denmark)

    Itenov, Theis Skovsgaard; Kirkby, Nikolai S; Bestle, Morten H;

    2015-01-01

    BACKGROUD: Hyaluronic acid (HA) is proposed as a marker of functional liver capacity. The aim of the present study was to compare a new turbidimetric assay for measuring HA with the current standard method. METHODS: HA was measured by a particle-enhanced turbidimetric immunoassay (PETIA) and enzyme...

  4. Instrument for assaying radiation

    Energy Technology Data Exchange (ETDEWEB)

    Coleman, Jody Rustyn; Farfan, Eduardo B.

    2016-03-22

    An instrument for assaying radiation includes a flat panel detector having a first side opposed to a second side. A collimated aperture covers at least a portion of the first side of the flat panel detector. At least one of a display screen or a radiation shield may cover at least a portion of the second side of the flat panel detector.

  5. Doing Diversity

    DEFF Research Database (Denmark)

    Just, Sine Nørholm; Christiansen, Tanja Juul

    2012-01-01

    invite audiences to take up subject positions, understood as combinations of identity and agency. Danish diversity management rhetoric functions as an illustrative example; in analyzing this type of rhetoric we show how subjects are called into restrained positions of similarity/difference and thereby...

  6. Generational diversity.

    Science.gov (United States)

    Kramer, Linda W

    2010-01-01

    Generational diversity has proven challenges for nurse leaders, and generational values may influence ideas about work and career planning. This article discusses generational gaps, influencing factors and support, and the various generational groups present in today's workplace as well as the consequences of need addressing these issues. The article ends with a discussion of possible solutions.

  7. Generational diversity.

    Science.gov (United States)

    Kramer, Linda W

    2010-01-01

    Generational diversity has proven challenges for nurse leaders, and generational values may influence ideas about work and career planning. This article discusses generational gaps, influencing factors and support, and the various generational groups present in today's workplace as well as the consequences of need addressing these issues. The article ends with a discussion of possible solutions. PMID:20395729

  8. Calibration of "Babyline" RP instruments

    CERN Multimedia

    2015-01-01

      If you have old RP instrumentation of the “Babyline” type, as shown in the photo, please contact the Radiation Protection Group (Joffrey Germa, 73171) to have the instrument checked and calibrated. Thank you. Radiation Protection Group

  9. Pressures Detector Calibration and Measurement

    CERN Document Server

    AUTHOR|(CDS)2156315

    2016-01-01

    This is report of my first and second projects (of 3) in NA61. I did data taking and analysis in order to do calibration of pressure detectors and verified it. I analyzed the data by ROOT software using the C ++ programming language. The first part of my project was determination of calibration factor of pressure sensors. Based on that result, I examined the relation between pressure drop, gas flow rate of in paper filter and its diameter.

  10. Beam Imaging and Luminosity Calibration

    CERN Document Server

    Klute, Markus; Salfeld-Nebgen, Jakob

    2016-01-01

    We discuss a method to reconstruct two-dimensional proton bunch densities using vertex distributions accumulated during LHC beam-beam scans. The $x$-$y$ correlations in the beam shapes are studied and an alternative luminosity calibration technique is introduced. We demonstrate the method on simulated beam-beam scans and estimate the uncertainty on the luminosity calibration associated to the beam-shape reconstruction to be below 1\\%.

  11. WFC3: UVIS Dark Calibration

    Science.gov (United States)

    Bourque, Matthew; Biretta, John A.; Anderson, Jay; Baggett, Sylvia M.; Gunning, Heather C.; MacKenty, John W.

    2014-06-01

    Wide Field Camera 3 (WFC3), a fourth-generation imaging instrument on board the Hubble Space Telescope (HST), has exhibited excellent performance since its installation during Servicing Mission 4 in May 2009. The UVIS detector, comprised of two e2v CCDs, is one of two channels available on WFC3 and is named for its ultraviolet and visible light sensitivity. We present the various procedures and results of the WFC3/UVIS dark calibration, which monitors the health and stability of the UVIS detector, provides characterization of hot pixels and dark current, and produces calibration files to be used as a correction for dark current in science images. We describe the long-term growth of hot pixels and the impacts that UVIS Charge Transfer Efficiency (CTE) losses, postflashing, and proximity to the readout amplifiers have on the population. We also discuss the evolution of the median dark current, which has been slowly increasing since the start of the mission and is currently ~6 e-/hr/pix, averaged across each chip. We outline the current algorithm for creating UVIS dark calibration files, which includes aggressive cosmic ray masking, image combination, and hot pixel flagging. Calibration products are available to the user community, typically 3-5 days after initial processing, through the Calibration Database System (CDBS). Finally, we discuss various improvements to the calibration and monitoring procedures. UVIS dark monitoring will continue throughout and beyond HST’s current proposal cycle.

  12. Incompatible Ages for Clearwing Butterflies Based on Alternative Secondary Calibrations.

    Science.gov (United States)

    Garzón-Orduña, Ivonne J; Silva-Brandão, Karina L; Willmott, Keith R; Freitas, André V L; Brower, Andrew V Z

    2015-09-01

    The recent publication of a time-tree for the plant family Solanaceae (nightshades) provides the opportunity to use independent calibrations to test divergence times previously inferred for the diverse Neotropical butterfly tribe Ithomiini. Ithomiini includes clades that are obligate herbivores of Solanaceae, with some genera feeding on only one genus. We used 8 calibrations extracted from the plant tree in a new relaxed molecular-clock analysis to produce an alternative temporal framework for the diversification of ithomiines. We compared the resulting age estimates to: (i) a time-tree obtained using 7 secondary calibrations from the Nymphalidae tree of Wahlberg et al. (2009), and (ii) Wahlberg et al.'s (2009) original age estimates for the same clades. We found that Bayesian clock estimates were rather sensitive to a variety of analytical parameters, including taxon sampling. Regardless of this sensitivity however, ithomiine divergence times calibrated with the ages of nightshades were always on average half the age of previous estimates. Younger dates for ithomiine clades appear to fit better with factors long suggested to have promoted diversification of the group such as the uplifting of the Andes, in the case of montane genera. Alternatively, if ithomiines are as old as previous estimates suggest, the recent ages inferred for the diversification of Solanaceae seem likely to be seriously underestimated. Our study exemplifies the difficulty of testing hypotheses of divergence times and of choosing between alternative dating scenarios, and shows that age estimates based on seemingly plausible calibrations may be grossly incongruent.

  13. Troubling Diversity?

    DEFF Research Database (Denmark)

    Jæger, Kirsten; Jensen, Annie Aarup

    2009-01-01

    Focussing on the cultural encounter between nurses and ethnic minority patients in Danish hospitals, this paper presents the results of a comprehensive analysis of nursing discourses on cultural difference and intercultural contact. Articles from the Danish professional journal ‘The Nurse......', published in the period from 2000 to 2008, pertaining to cultural contact and intercultural understanding have been analyzed in order to uncover nurses' experience of ethnic and cultural diversity and the ways, in which these experiences challenge their cultural and professional expertise. Results...... are related to recent contributions to diversity management theory and intercultural communication theory, calling for a strengthened focus on the historical, political, and social dimensions of intercultural contact. In continuation of these trends, an alternative, theoretical framework...

  14. Non-Destructive Assay of Curium Contaminated Transuranic Waste Drums

    Energy Technology Data Exchange (ETDEWEB)

    Foster, L.A.

    1998-11-01

    At the Plutonium Facility at Los Alamos National Laboratory, a series of non-destructive assays were performed on five transuranic waste (TRU) drums containing non-plutonium scrap metal that was potentially contaminated with weapons grade plutonium and trace quantities of curium. Typically, waste drums containing metal matrices are assayed for plutonium content using passive neutron coincidence counting techniques. The presence of trace quantities of Cm-244 prevents this type of analysis because of the strong coincidence signal created by spontaneous fission of Cm-244. To discriminate between the plutonium and curium materials present, an active neutron measurement technique was used. A Cf shuffler designed for measurement of uranium bearing materials was calibrated for plutonium in the active mode. The waste drums were then assayed for plutonium content in the shuffler using the active-mode calibration. The curium contamination levels were estimated from the difference between the active-mode measurement in the shuffler and a passive assay in a neutron coincidence counter. Far field gamma-ray measurements were made to identify additional radioactive contaminants and to corroborate the plutonium measurement results obtained from the active-mode assay. This report describes in detail the measurement process used for characterization of these waste drums. The measurement results and the estimated uncertainty will be presented.

  15. Specific and global coagulation assays in the diagnosis of discrepant mild hemophilia A.

    Science.gov (United States)

    Bowyer, Annette E; Van Veen, Joost J; Goodeve, Anne C; Kitchen, Steve; Makris, Michael

    2013-12-01

    The activity of the factor VIII coagulation protein can be measured by three methods: a one or two-stage clotting assay and a chromogenic assay. The factor VIII activity of most individuals with mild hemophilia A is the same regardless of which method is employed. However, approximately 30% of patients show marked discrepancies in factor VIII activity measured with the different methods. The objective of this study was to investigate the incidence of assay discrepancy in our center, assess the impact of alternative reagents on factor VIII activity assays and determine the usefulness of global assays of hemostasis in mild hemophilia A. Factor VIII activity was measured in 84 individuals with mild hemophilia A using different reagents. Assay discrepancy was defined as a two-fold or greater difference between the results of the one-stage and two-stage clotting assays. Rotational thromboelastometry and calibrated automated thrombography were performed. Assay discrepancy was observed in 31% of individuals; 12% with lower activity in the two-stage assay and 19% with lower activity in the one-stage assay. The phenotype could not always be predicted from the individual's genotype. Chromogenic assays were shown to be a suitable alternative to the two-stage clotting assay. Thromboelastometry was found to have poor sensitivity in hemophilia. Calibrated automated thrombography supported the results obtained by the two-stage and chromogenic assays. The current international guidelines do not define the type of assay to be used in the diagnosis of mild hemophilia A and some patients could be misclassified as normal. In our study, 4% of patients would not have been diagnosed on the basis of the one-stage factor VIII assay. Laboratories should use both one stage and chromogenic (or two-stage) assays in the diagnosis of patients with possible hemophilia A.

  16. Managing diversity.

    Science.gov (United States)

    Wagner, M

    1991-09-30

    One look at projections for the U.S. work force through the year 2000 shows why healthcare administrators will be facing some new challenges. With the majority of new workers belonging to minority groups, "managing diversity" has become the newest catch phrase as executives work to reduce tensions resulting from race, gender or culture-based differences among workers, while also learning to understand and value those differences.

  17. Mathematical calibration of Ge detectors, and the instruments that use them

    Energy Technology Data Exchange (ETDEWEB)

    Bronson, F.L.; Young, B. [Canberra Industries, Meriden, CT (United States)

    1997-11-01

    Efficiency calibrations for Ge detectors are typically done with the use of multiple energy calibrations sources which are added to a bulk matrix intended to simulate the measurement sample, and then deposited in the sample container. This is rather easy for common laboratory samples. Bu, even there, for many environmental samples, waste assay samples, and operational health physics samples, accurate calibrations are difficult. For these situations, various mathematical corrections or direct calibration techniques are used at Canberra. EML has pioneered the use of mathematical calibrations following source-based detector characterization measurements for in situ measurements of environmental fallout. Canberra has expanded this by the use of MCNP for the source measurements required in EML. For other calibration situations, MCNP was used directly, as the primary calibration method. This is demonstrated to be at least as accurate as source based measurements, and probably better. Recently, a new method [ISOCS] has been developed and is nearing completion. This promises to be an easy to use calibration software that can be used by the customer for in situ gamma spectroscopy to accurately measure many large sized samples, such as boxes, drums, pipes, or to calibrate small laboratory-type samples. 8 refs., 8 figs., 5 tabs.

  18. Disagreement between Human Papillomavirus Assays

    DEFF Research Database (Denmark)

    Rebolj, Matejka; Preisler, Sarah; Ejegod, Ditte Møller;

    2014-01-01

    We aimed to determine the disagreement in primary cervical screening between four human papillomavirus assays: Hybrid Capture 2, cobas, CLART, and APTIMA. Material from 5,064 SurePath samples of women participating in routine cervical screening in Copenhagen, Denmark, was tested with the four...... assays. Positive agreement between the assays was measured as the conditional probability that the results of all compared assays were positive given that at least one assay returned a positive result. Of all 5,064 samples, 1,679 (33.2%) tested positive on at least one of the assays. Among these, 41......-65 years (n = 2,881), 23% tested positive on at least one assay, and 42 to 58% of these showed positive agreement on any compared pair of the assays. While 4% of primary screening samples showed abnormal cytology, 6 to 10% were discordant on any pair of assays. A literature review corroborated our findings...

  19. Advances in Assays and Analytical Approaches for Botulinum Toxin Detection

    Energy Technology Data Exchange (ETDEWEB)

    Grate, Jay W.; Ozanich, Richard M.; Warner, Marvin G.; Bruckner-Lea, Cindy J.; Marks, James D.

    2010-08-04

    Methods to detect botulinum toxin, the most poisonous substance known, are reviewed. Current assays are being developed with two main objectives in mind: 1) to obtain sufficiently low detection limits to replace the mouse bioassay with an in vitro assay, and 2) to develop rapid assays for screening purposes that are as sensitive as possible while requiring an hour or less to process the sample an obtain the result. This review emphasizes the diverse analytical approaches and devices that have been developed over the last decade, while also briefly reviewing representative older immunoassays to provide background and context.

  20. The Matrigel Cytokine Assay

    OpenAIRE

    sprotocols

    2015-01-01

    Authors: Ole Audun Haabeth, Bjarne Bogen & Alexandre Corthay ### Abstract Cytokines represent a diverse group of soluble proteins that play a key role in a number of physiological processes, including regulation of both the innate and adaptive immune responses. Cytokines are generally secreted in small amounts, and are relatively unstable. Therefore, detection and measurement of cytokine local concentrations in a tissue extracellular matrix can be challenging. To investigate cytok...

  1. The KLOE Online Calibration System

    Institute of Scientific and Technical Information of China (English)

    E.Pasqualucci

    2001-01-01

    Based on all the features of the KLOE online software,the online calibration system performs current calibration quality checking in real time and starts automatically new calibration procedures when needed.Acalibration manager process controls the system,implementing the interface to the online system,receiving information from the run control and translating its state transitions to a separate state machine.It acts as a " calibration run controller"and performs failure recovery when requested by a set of process checkers.The core of the system is a multi-threaded OO histogram server that receives histogramming commands by remote processes and operates on local ROOT histograms.A client library and C,fortran and C++ application interface libraries allow the user to connect and define his own histogram or read histograms owned by others using an bool-like interface.Several calibration processes running in parallel in a destributed,multiplatform environment can fill the same histograms,allowing fast external information check.A monitor thread allow remote browsing for visual inspection,Pre-filtered data are read in nonprivileged spy mode from the data acquisition system via the Kloe Integrated Dataflow,privileged spy mode from the data acquisiton system via the Kole Integrated Dataflow.The main characteristics of the system are presented.

  2. Nondestructive analysis of nuclear materials by isotopic source assay system

    International Nuclear Information System (INIS)

    Destructive assay is an effective method for the analysis of nuclear materials in nuclear fuels, but is not suitable for some aspects of nuclear fuel cycle, for example, for accounting and control or safeguard purposes. Isotopic Source Assay System was imported from Intelcom Rad. Tech. Company, and the results of assay of enriched uranium and plutonium sealed for passive and active assay by the system are presented. A 252Cf source is provided for the assay system. Assay of unknown samples by this system is carried out relatively to the measurement of known standards. Several known standards approximating the physical and chemical properties of unknown samples are prepared to make calibration curves. When one fission event occurs in a sample, a few neutrons (2.5 neutrons on the average) and gamma ray (about 7 photons) are emitted simultaneously. By three detector coincidence out of four, one count is registered by the assay system. First, statistical informations and geometry were examined. Then, three kinds of enriched uranium were measured to examine the measurement on 238U. Passive and active measurements were performed on 4.32 grams of PuO2 during one month to know reproducibility. In conclusion of these tests, it was proved to be able to apply this system to the analysis of nuclear materials similar in enrichment or isotopic composition, and scraps and wastes containing known matrix materials. (Wakatsuki, Y.)

  3. Robust versatile tyrosine kinase assay for HTS in drug discovery

    Science.gov (United States)

    Deshpande, Sudhir S.; Mineyev, I.; Owicki, John C.

    1999-04-01

    A fluorescence polarization assay was developed as an alternative to the radiolabeled SPA assays currently used to monitor the activity of tyrosine kinases in drug discovery. The assay can be used with enzymes having substrate specificity similar to that of the insulin receptor, the EGF receptor and the Src kinase receptor enzymes. The assay is easy to configure in 96, 384 and 1536-well microplates in assay volumes ranging from (mu) L with minimal efforts. The reconstituted reagents are stable for up to 24 hr at ambient temperatures, thereby minimizing the need for replenishing the stock solutions during the course of a high-throughput screen. Because of the stability and equilibrium kinetics, the assay allows the user the luxury of scheduling the reading of plates any time up to 24 hr after the completion of the assay without substantial deterioration in the assay signal. The antibody and the tracer solutions can also be premixed and added as a preformed complex in a single step. The performance of the assay with the insulin receptor kinase is described. In addition, given the diversity of the substrates used in measuring the activity of different tyrosine kinases, LJL's on-going efforts to provide different antibodies of wide ranging specificity and sensitivity are described.

  4. Radon assay for SNO+

    International Nuclear Information System (INIS)

    The SNO+ experiment will study neutrinos while located 6,800 feet below the surface of the earth at SNOLAB. Though shielded from surface backgrounds, emanation of radon radioisotopes from the surrounding rock leads to back-grounds. The characteristic decay of radon and its daughters allows for an alpha detection technique to count the amount of Rn-222 atoms collected. Traps can collect Rn-222 from various positions and materials, including an assay skid that will collect Rn-222 from the organic liquid scintillator used to detect interactions within SNO+

  5. FLUIDICS DEVICE FOR ASSAY

    DEFF Research Database (Denmark)

    2007-01-01

    The present invention relates to a device for use in performing assays on standard laboratory solid supports whereon chemical entities are attached. The invention furthermore relates to the use of such a device and a kit comprising such a device. The device according to the present invention is a......, when operatively connected, one or more chambers (21) comprising the chemical entities (41), the inlet(s) (5) and outlet(s) (6) and chambers (21) being in fluid connection. The device further comprise means for providing differing chemical conditions in each chamber (21)....

  6. Growth cone collapse assay.

    Science.gov (United States)

    Cook, Geoffrey M W; Jareonsettasin, Prem; Keynes, Roger J

    2014-01-01

    The growth cone collapse assay has proved invaluable in detecting and purifying axonal repellents. Glycoproteins/proteins present in detergent extracts of biological tissues are incorporated into liposomes, added to growth cones in culture and changes in morphology are then assessed. Alternatively purified or recombinant molecules in aqueous solution may be added directly to the cultures. In both cases after a defined period of time (up to 1 h), the cultures are fixed and then assessed by inverted phase contrast microscopy for the percentage of growth cones showing a collapsed profile with loss of flattened morphology, filopodia, and lamellipodia.

  7. Radon assay for SNO+

    Energy Technology Data Exchange (ETDEWEB)

    Rumleskie, Janet [Laurentian University, Greater Sudbury, Ontario (Canada)

    2015-12-31

    The SNO+ experiment will study neutrinos while located 6,800 feet below the surface of the earth at SNOLAB. Though shielded from surface backgrounds, emanation of radon radioisotopes from the surrounding rock leads to back-grounds. The characteristic decay of radon and its daughters allows for an alpha detection technique to count the amount of Rn-222 atoms collected. Traps can collect Rn-222 from various positions and materials, including an assay skid that will collect Rn-222 from the organic liquid scintillator used to detect interactions within SNO+.

  8. Stable isotope dilution assays in mycotoxin analysis

    Energy Technology Data Exchange (ETDEWEB)

    Rychlik, Michael; Asam, Stefan [Universitaet Muenchen, Lehrstuhl fuer Lebensmittelchemie der Technischen, Garching (Germany)

    2008-01-15

    The principle and applications of stable isotope dilution assays (SIDAs) in mycotoxin analysis are critically reviewed. The general section includes historical aspects of SIDAs, the prerequisites and limitations of the use of stable isotopically labelled internal standards, and possible calibration procedures. In the application section actual SIDAs for the analysis of trichothecenes, zearalenone, fumonisins, patulin, and ochratoxin A are presented. The syntheses and availability of labelled mycotoxins for use as internal standards is reviewed and specific advances in food analysis and toxicology are demonstrated. The review indicates that LC-MS applications, in particular, require the use of stable isotopically labelled standards to compensate for losses during clean-up and for discrimination due to ion suppression. As the commercial availability of these compounds continues to increase, SIDAs can be expected to find expanding use in mycotoxin analysis. (orig.)

  9. Power calibrations for TRIGA reactors

    International Nuclear Information System (INIS)

    The purpose of this paper is to establish a framework for the calorimetric power calibration of TRIGA reactors so that reliable results can be obtained with a precision better than ± 5%. Careful application of the same procedures has produced power calibration results that have been reproducible to ± 1.5%. The procedures are equally applicable to the Mark I, Mark II and Mark III reactors as well as to reactors having much larger reactor tanks and to TRIGA reactors capable of forced cooling up to 3 MW in some cases and 15 MW in another case. In the case of forced cooled TRIGA reactors, the calorimetric power calibration is applicable in the natural convection mode for these reactors using exactly the same procedures as are discussed below for the smaller TRIGA reactors (< 2 MW)

  10. Facility for dosimetric instrument calibration

    International Nuclear Information System (INIS)

    A structure is designed consisting of a rotary support of containers with radiation sources and of a rotary plug mounted above the sources. A support post with a slide rest and arms rotating around the post longitudinal axis is mounted in the centre of the container support. THe arms support instruments to be calibrated. The colimation cone of the respective source is directed to the tensor of the instrument being calibrated. The slide rest is balanced using a counterpoise mounted in the support post. Sources are not removed from the containers in source change during measurement. The support can hold more containers and the slide rest can support more instruments to be calibrated than the existing configurations. (M.D.). 2 figs

  11. NASA's Metrology and Calibration program

    Science.gov (United States)

    Kern, Frederick A.

    1991-01-01

    The success of the National Aeronautics and Space Administration's scientific and engineering projects is often based on the capability and quality of the metrology and calibration programs of its field centers and facilities. A Metrology and Calibration Working Group, having representation from each of the NASA field centers and facilities, was established to develop and implement a program to provide a centralized direction and agency wide focus. A brief history of the Group is presented. The development of the Group's operating philosophy, the long term objectives, the measurement research and development program, and review of accomplishments is discussed.

  12. GREAT/SOFIA atmospheric calibration

    CERN Document Server

    Guan, Xin; Graf, Urs U; Güsten, Rolf; Okada, Yoko; Torres, Miguel Angel Requena; Simon, Robert; Wiesemeyer, Helmut

    2012-01-01

    The GREAT observations need frequency-selective calibration across the passband for the residual atmospheric opacity at flight altitude. At these altitudes the atmospheric opacity has both narrow and broad spectral features. To determine the atmospheric transmission at high spectral resolution, GREAT compares the observed atmospheric emission with atmospheric model predictions, and therefore depends on the validity of the atmospheric models. We discusse the problems identified in this comparison with respect to the observed data and the models, and describe the strategy used to calibrate the science data from GREAT/SOFIA during the first observing periods.

  13. Field calibration of cup anemometers

    DEFF Research Database (Denmark)

    Kristensen, L.; Jensen, G.; Hansen, A.;

    2001-01-01

    . It is concluded that the method has the advantage that many anemometers can be calibrated accurately with a minimum of work and cost. The obvious disadvantage is that the calibration of a set of anemometersmay take more than one month in order to have wind speeds covering a sufficiently large magnitude range...... in a wind direction sector where we can be sure that the instruments are exposed to identical, simultaneous wind flows. Another main conclusion is thatstatistical uncertainty must be carefully evaluated since the individual 10 minute wind-speed averages are not statistically independent....

  14. Performance standard for dose Calibrator

    CERN Document Server

    Darmawati, S

    2002-01-01

    Dose calibrator is an instrument used in hospitals to determine the activity of radionuclide for nuclear medicine purposes. International Electrotechnical Commission (IEC) has published IEC 1303:1994 standard that can be used as guidance to test the performance of the instrument. This paper briefly describes content of the document,as well as explains the assessment that had been carried out to test the instrument accuracy in Indonesia through intercomparison measurement.Its is suggested that hospitals acquire a medical physicist to perform the test for its dose calibrator. The need for performance standard in the form of Indonesia Standard is also touched.

  15. Tank calibration; Arqueacao de tanques

    Energy Technology Data Exchange (ETDEWEB)

    Chan, Ana [PETROBRAS S.A., Rio de Janeiro, RJ (Brazil)

    2003-07-01

    This work relates the analysis of the norms ISO (International Organization for Standardization) for calibration of vertical cylindrical tanks used in fiscal measurement, established on Joint Regulation no 1 of June 19, 2000 between the ANP (National Agency of Petroleum) and the INMETRO (National Institute of Metrology, Normalization and Industrial Quality). In this work a comparison between norms ISO and norms published by the API (American Petroleum Institute) and the IP (Institute of Petroleum) up to 2001 was made. It was concluded that norms ISO are wider than norms API, IP, and INMETRO methods in the calibration of vertical cylindrical tanks. (author)

  16. Optimizing diversity

    CERN Document Server

    Frein, Yannick; Sebo, Andras

    2007-01-01

    We consider the problem of minimizing the size of a family of sets G such that every subset of 1,...,n can be written as a disjoint union of at most k members of G, where k and n are given numbers. This problem originates in a real-world application aiming at the diversity of industrial production. At the same time, the minimum of G so that every subset of 1,...,n is the union of two sets in G has been asked by Erdos and studied recently by Furedi and Katona without requiring the disjointness of the sets. A simple construction providing a feasible solution is conjectured to be optimal for this problem for all values of n and k and regardless of the disjointness requirement; we prove this conjecture in special cases including all (n,k) for which n <= 3k holds, and some individual values of n and k.

  17. Design Diversity

    DEFF Research Database (Denmark)

    Dankl, Kathrina

    2014-01-01

    The publication 'Design Diversity', an exhibition catalogue, focuses on aging and design – a product culture in transformation that aims to help change conventional notions of the later years of life. Age is positioned as a generational issue that has the same relevance for all age groups...... courageous projects for "best agers" and "golden agers" never get beyond the prototype stage, products that paint a more "beige" picture of everyday life can be found in large numbers. This fact raises some key questions: Does the existing product culture reflect today's views on old age? Do contemporary...... awareness of the fact that material culture shapes our view of aging, and therefore is also capable of changing it....

  18. Calibration Report for the WRAP Facility Gamma Energy Analysis System [104-ND-06-102A

    Energy Technology Data Exchange (ETDEWEB)

    WILLS, C.E.

    2000-02-15

    The Waste Receiving And Processing facility (WRAP) adheres to providing gamma-ray spectroscopy instrument calibrations traceable to the National Institute for Standards and Technology (NIST) standards. The detectors are used to produce quantitative results for the Waste Isolation Pilot Plant (WIPP) and must meet calibration programmatic calibration goals. Instruments must meet portions of ANSI N42.14, 1978 guide for Germanium detectors. The Non-Destructive Assay (NDA) Gamma Energy Analysis (GEA) utilizes NIST traceable line source standards for the detector system calibrations. The counting configuration is a series of drums containing the line sources and different density filler matrices. The drums are used to develop system efficiencies with respect to density. The efficiency and density correction factors are required for the processing of drummed waste materials of similar densities. The calibration verification is carried out after the calibration is deemed final, by counting a second drum of NIST traceable sources. Three in-depth calibrations have been completed on one of the two systems to date, the first being the system acceptance plan. This report has a secondary function; that being the development of the instrument calibration errors which are to be folded into the Total Instrument Uncertainty document, HNF-4050.

  19. Calibration Report for the WRAP Facility Gamma Energy Analysis System (104-ND-06-102A)

    Energy Technology Data Exchange (ETDEWEB)

    WILLS, C.E.

    2000-03-13

    The Waste Receiving And Processing facility (WRAP) adheres to providing gamma-ray spectroscopy instrument calibrations traceable to the National Institute for Standards and Technology (NIST) standard{sup (4)}. The detectors are used to produce quantitative results for the Waste Isolation Pilot Plant (WIPP) and must meet calibration programmatic calibration goals. Instruments must meet portions of ANSI N42.14, 1978 guide for Germanium detectors. The Non-Destructive Assay (NDA) Gamma Energy Analysis (GEA) utilizes NIST traceable line source standards for the detector system calibrations. The counting configuration is a series of drums containing the line sources and different density filler matrices. The drums are used to develop system efficiencies with respect to density. The efficiency and density correction factors are required for the processing of drummed waste materials of similar densities. The calibration verification is carried out after the calibration is deemed final, by counting a second drum of NIST traceable sources. Three in-depth calibrations have been completed on one of the two systems to date, the first being the system acceptance plan. This report has a secondary function; that being the development of the instrument calibration errors which are to be folded into the Total Instrument Uncertainty document, HNF-4050.

  20. Recommended Inorganic Chemicals for Calibration.

    Science.gov (United States)

    Moody, John R.; And Others

    1988-01-01

    All analytical techniques depend on the use of calibration chemicals to relate analyte concentration to instrumental parameters. Discusses the preparation of standard solutions and provides a critical evaluation of available materials. Lists elements by group and discusses the purity and uses of each. (MVL)

  1. Reliability-Based Code Calibration

    DEFF Research Database (Denmark)

    Faber, M.H.; Sørensen, John Dalsgaard

    2003-01-01

    . Thereafter the code calibration problem is presented in its principal decision theoretical form and it is discussed how acceptable levels of failure probability (or target reliabilities) may be established. Furthermore suggested values for acceptable annual failure probabilities are given for ultimate...

  2. Measurement System and Calibration report

    DEFF Research Database (Denmark)

    Kock, Carsten Weber; Vesth, Allan

    This Measurement System & Calibration report is describing DTU’s measurement system installed at a specific wind turbine. A major part of the sensors has been installed by others (see [1]) the rest of the sensors have been installed by DTU. The results of the measurements, described in this report...

  3. Measurement System and Calibration report

    DEFF Research Database (Denmark)

    Vesth, Allan; Kock, Carsten Weber

    The report describes power curve measurements carried out on a given wind turbine. The measurements are carried out in accordance to Ref. [1]. A site calibration has been carried out; see Ref. [2], and the measured flow correction factors for different wind directions are used in the present...

  4. Scalar Calibration of Vector Magnetometers

    DEFF Research Database (Denmark)

    Merayo, José M.G.; Brauer, Peter; Primdahl, Fritz;

    2000-01-01

    the parameters for a given data set. Therefore, a magnetometer may be characterized inexpensively in the Earth's magnetic-field environment. This procedure has been used successfully in the pre-flight calibration of the state-of-the-art magnetometers on board the magnetic mapping satellites Orsted, Astrid-2...

  5. Calibration and Characterization of the Small Sample Calorimeter

    Energy Technology Data Exchange (ETDEWEB)

    Santi, Peter A. [Los Alamos National Laboratory; Perry, Katherine A. [Los Alamos National Laboratory

    2012-08-13

    An early component of the Joint Fuel Cycle Study (JFCS) between the United States and the Republic of Korea is a test of gram scale electrochemical recycling of spent fuel which is to be performed at Idaho National Laboratory (INL). Included in this test is the development of Nondestructive Assay (NDA) technologies that would be applicable for International Atomic Energy Agency (IAEA) safeguards of the electrochemical recycling process. Of upmost importance to safeguarding the fuel cycle associated with the electrochemical recycling process is the ability to safeguard the U/TRU ingots that will be produced in the process. For the gram scale test, the ingots that will be produced will have an expected thermal power of approximately 130 mW. To ascertain how well the calorimetric assay NDA technique can perform in assaying these ingots, Los Alamos National Laboratory (LANL) has characterized and calibrated a small solid-state calorimeter called the Small Sample Calorimeter (SSC3) to perform these measurements at LANL. To calibrate and characterize the SSC3, a series of measurements were performed using certified {sup 238}Pu heat standards whose power output is traceable back to the National Institute of Standards and Technology (NIST) electrical standards. The results of these measurements helped establish both the calibration of the calorimeter as well as the expected performance of the calorimeter in terms of its accuracy and precision as a function of thermal power of the item that is being measured. In this report, we will describe the measurements that were performed and provide a discussion of the results of these measurements.

  6. Radio Interferometric Calibration Using a Riemannian Manifold

    CERN Document Server

    Yatawatta, Sarod

    2013-01-01

    In order to cope with the increased data volumes generated by modern radio interferometers such as LOFAR (Low Frequency Array) or SKA (Square Kilometre Array), fast and efficient calibration algorithms are essential. Traditional radio interferometric calibration is performed using nonlinear optimization techniques such as the Levenberg-Marquardt algorithm in Euclidean space. In this paper, we reformulate radio interferometric calibration as a nonlinear optimization problem on a Riemannian manifold. The reformulated calibration problem is solved using the Riemannian trust-region method. We show that calibration on a Riemannian manifold has faster convergence with reduced computational cost compared to conventional calibration in Euclidean space.

  7. NIR calibration of soluble stem carbohydrates for predicting drought tolerance in spring wheat

    Science.gov (United States)

    Soluble stem carbohydrates are a component of drought response in wheat (Triticum aestivum L.) and other grasses. Near-infrared spectroscopy (NIR) can rapidly assay for soluble carbohydrates indirectly, but this requires a statistical model for calibration. The objectives of this study were: (i) to ...

  8. Calibration and seasonal adjustment for matched case-control studies of vitamin D and cancer.

    Science.gov (United States)

    Gail, Mitchell H; Wu, Jincao; Wang, Molin; Yaun, Shiaw-Shyuan; Cook, Nancy R; Eliassen, A Heather; McCullough, Marjorie L; Yu, Kai; Zeleniuch-Jacquotte, Anne; Smith-Warner, Stephanie A; Ziegler, Regina G; Carroll, Raymond J

    2016-06-15

    Vitamin D measurements are influenced by seasonal variation and specific assay used. Motivated by multicenter studies of associations of vitamin D with cancer, we formulated an analytic framework for matched case-control data that accounts for seasonal variation and calibrates to a reference assay. Calibration data were obtained from controls sampled within decile strata of the uncalibrated vitamin D values. Seasonal sine-cosine series were fit to control data. Practical findings included the following: (1) failure to adjust for season and calibrate increased variance, bias, and mean square error and (2) analysis of continuous vitamin D requires a variance adjustment for variation in the calibration estimate. An advantage of the continuous linear risk model is that results are independent of the reference date for seasonal adjustment. (3) For categorical risk models, procedures based on categorizing the seasonally adjusted and calibrated vitamin D have near nominal operating characteristics; estimates of log odds ratios are not robust to choice of seasonal reference date, however. Thus, public health recommendations based on categories of vitamin D should also define the time of year to which they refer. This work supports the use of simple methods for calibration and seasonal adjustment and is informing analytic approaches for the multicenter Vitamin D Pooling Project for Breast and Colorectal Cancer. Published 2016. This article has been contributed to by US Government employees and their work is in the public domain in the USA. PMID:27133461

  9. New NIR Calibration Models Speed Biomass Composition and Reactivity Characterization

    Energy Technology Data Exchange (ETDEWEB)

    2015-09-01

    Obtaining accurate chemical composition and reactivity (measures of carbohydrate release and yield) information for biomass feedstocks in a timely manner is necessary for the commercialization of biofuels. This highlight describes NREL's work to use near-infrared (NIR) spectroscopy and partial least squares multivariate analysis to develop calibration models to predict the feedstock composition and the release and yield of soluble carbohydrates generated by a bench-scale dilute acid pretreatment and enzymatic hydrolysis assay. This highlight is being developed for the September 2015 Alliance S&T Board meeting.

  10. Hyperpolarized NMR Probes for Biological Assays

    Directory of Open Access Journals (Sweden)

    Sebastian Meier

    2014-01-01

    Full Text Available During the last decade, the development of nuclear spin polarization enhanced (hyperpolarized molecular probes has opened up new opportunities for studying the inner workings of living cells in real time. The hyperpolarized probes are produced ex situ, introduced into biological systems and detected with high sensitivity and contrast against background signals using high resolution NMR spectroscopy. A variety of natural, derivatized and designed hyperpolarized probes has emerged for diverse biological studies including assays of intracellular reaction progression, pathway kinetics, probe uptake and export, pH, redox state, reactive oxygen species, ion concentrations, drug efficacy or oncogenic signaling. These probes are readily used directly under natural conditions in biofluids and are often directly developed and optimized for cellular assays, thus leaving little doubt about their specificity and utility under biologically relevant conditions. Hyperpolarized molecular probes for biological NMR spectroscopy enable the unbiased detection of complex processes by virtue of the high spectral resolution, structural specificity and quantifiability of NMR signals. Here, we provide a survey of strategies used for the selection, design and use of hyperpolarized NMR probes in biological assays, and describe current limitations and developments.

  11. PathogenMip Assay: A Multiplex Pathogen Detection Assay

    OpenAIRE

    Akhras, Michael S.; Sreedevi Thiyagarajan; Villablanca, Andrea C.; Davis, Ronald W; Pål Nyrén; Nader Pourmand

    2007-01-01

    The Molecular Inversion Probe (MIP) assay has been previously applied to a large-scale human SNP detection. Here we describe the PathogenMip Assay, a complete protocol for probe production and applied approaches to pathogen detection. We have demonstrated the utility of this assay with an initial set of 24 probes targeting the most clinically relevant HPV genotypes associated with cervical cancer progression. Probe construction was based on a novel, cost-effective, ligase-based protocol. The ...

  12. Radiometric Calibration of Osmi Imagery Using Solar Calibration

    Science.gov (United States)

    Lee, Dong-Han; Kim, Yong-Seung

    2000-12-01

    OSMI (Ocean Scanning Multi-Spectral Imager) raw image data (Level 0) were acquired and radiometrically corrected. We have applied two methods, using solar & dark calibration data from OSMI sensor and comparing with the SeaWiFS data, to the radiometric correction of OSMI raw image data. First, we could get the values of the gain and the offset for each pixel and each band from comparing the solar & dark calibration data with the solar input radiance values, calculated from the transmittance, BRDF (Bidirectional Reflectance Distribution Function) and the solar incidence angle (¥â,¥è) of OSMI sensor. Applying this calibration data to OSMI raw image data, we got the two odd results, the lower value of the radiometric corrected image data than the expected value, and the Venetian Blind Effect in the radiometric corrected image data. Second, we could get the reasonable results from comparing OSMI raw image data with the SeaWiFS data, and get a new problem of OSMI sensor.

  13. SMAP RADAR Calibration and Validation

    Science.gov (United States)

    West, R. D.; Jaruwatanadilok, S.; Chaubel, M. J.; Spencer, M.; Chan, S. F.; Chen, C. W.; Fore, A.

    2015-12-01

    The Soil Moisture Active Passive (SMAP) mission launched on Jan 31, 2015. The mission employs L-band radar and radiometer measurements to estimate soil moisture with 4% volumetric accuracy at a resolution of 10 km, and freeze-thaw state at a resolution of 1-3 km. Immediately following launch, there was a three month instrument checkout period, followed by six months of level 1 (L1) calibration and validation. In this presentation, we will discuss the calibration and validation activities and results for the L1 radar data. Early SMAP radar data were used to check commanded timing parameters, and to work out issues in the low- and high-resolution radar processors. From April 3-13 the radar collected receive only mode data to conduct a survey of RFI sources. Analysis of the RFI environment led to a preferred operating frequency. The RFI survey data were also used to validate noise subtraction and scaling operations in the radar processors. Normal radar operations resumed on April 13. All radar data were examined closely for image quality and calibration issues which led to improvements in the radar data products for the beta release at the end of July. Radar data were used to determine and correct for small biases in the reported spacecraft attitude. Geo-location was validated against coastline positions and the known positions of corner reflectors. Residual errors at the time of the beta release are about 350 m. Intra-swath biases in the high-resolution backscatter images are reduced to less than 0.3 dB for all polarizations. Radiometric cross-calibration with Aquarius was performed using areas of the Amazon rain forest. Cross-calibration was also examined using ocean data from the low-resolution processor and comparing with the Aquarius wind model function. Using all a-priori calibration constants provided good results with co-polarized measurements matching to better than 1 dB, and cross-polarized measurements matching to about 1 dB in the beta release. During the

  14. Calibration of the solar radio spectrometer

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    This paper shows some improvements and new results of calibration of Chinese solar radio spectrometer by analyzing the daily calibration data recorded in the period of 1997-2007. First, the calibration coefficient is fitted for three bands (1.0-2.0 GHz, 2.6-3.8 GHz, 5.2-7.6 GHz) of the spectrometer by using the moving-average method confined by the property of the daily calibration data. By this calibration coefficient, the standard deviation of the calibration result was less than 10 sfu for 95% frequencies of 2.6-3.8 GHz band in 2003. This result is better than that calibrated with the constant coefficient. Second, the calibration coefficient is found in good correlation with local air temperature for most frequencies of 2.6-3.8 GHz band. Moreover, these results are helpful in the research of the quiet solar radio emission.

  15. Calibration of the solar radio spectrometer

    Institute of Scientific and Technical Information of China (English)

    TAN ChengMing; YAN YiHua; TAN BaoLin; XU GuiRong

    2009-01-01

    This paper shows some improvements and new results of calibration of Chinese solar radio spectrom-eter by analyzing the daily calibration data recorded in the period of 1997-2007. First, the calibration coefficient is fitted for three bands (1.0-2.0 GHz, 2.6-3.8 GHz, 5.2-7.6 GHz) of the spectrometer by using the moving-average method confined by the property of the daily calibration data. By this calibration coefficient, the standard deviation of the calibration result was less than 10 sfu for 95% frequencies of 2.6-3.8 GHz band in 2003. This result is better than that calibrated with the constant coefficient. Second, the calibration coefficient is found in good correlation with local air temperature for most frequencies of 2.6-3.8 GHz band. Moreover, these results are helpful in the research of the quiet solar radio emission.

  16. Attitude Sensor and Gyro Calibration for Messenger

    Science.gov (United States)

    O'Shaughnessy, Daniel; Pittelkau, Mark E.

    2007-01-01

    The Redundant Inertial Measurement Unit Attitude Determination/Calibration (RADICAL(TM)) filter was used to estimate star tracker and gyro calibration parameters using MESSENGER telemetry data from three calibration events. We present an overview of the MESSENGER attitude sensors and their configuration is given, the calibration maneuvers are described, the results are compared with previous calibrations, and variations and trends in the estimated calibration parameters are examined. The warm restart and covariance bump features of the RADICAL(TM) filter were used to estimate calibration parameters from two disjoint telemetry streams. Results show that the calibration parameters converge faster with much less transient variation during convergence than when the filter is cold-started at the start of each telemetry stream.

  17. Crop physiology calibration in the CLM

    Directory of Open Access Journals (Sweden)

    I. Bilionis

    2015-04-01

    scalable and adaptive scheme based on sequential Monte Carlo (SMC. The model showed significant improvement of crop productivity with the new calibrated parameters. We demonstrate that the calibrated parameters are applicable across alternative years and different sites.

  18. Calibration and Validation of Measurement System

    DEFF Research Database (Denmark)

    Kofoed, Jens Peter; Riemann, Sven; Knapp, Wilfried

    The report deals with the calibration of the measuring equipment on board the Wave Dragon, Nissum Bredning prototype.......The report deals with the calibration of the measuring equipment on board the Wave Dragon, Nissum Bredning prototype....

  19. Astrid-2 EMMA Magnetic Calibration

    DEFF Research Database (Denmark)

    Merayo, José M.G.; Brauer, Peter; Risbo, Torben;

    1998-01-01

    The Swedish micro-satellite Astrid-2 contains a tri-axial fluxgate magnetometer with the sensor co-located with a Technical University of Denmark (DTU) star camera for absolute attitude, and extended about 0.9 m on a hinged boom. The magnetometer is part of the RIT EMMA electric and magnetic fields...... experiment built as a collaboration between the DTU, Department of Automation and the Department of Plasma Physics, The Alfvenlaboratory, Royal Institute of Technology (RIT), Stockholm. The final magnetic calibration of the Astrid-2 satellite was done at the Lovoe Magnetic Observatory under the Geological...... Survey of Sweden near Stockholm on the night of May 15.-16., 1997. The magnetic calibration and the intercalibration between the star camera and the magnetic sensor was performed by measuring the Earth's magnetic field and simultaneously observing the star sky with the camera. The rotation matrix between...

  20. Calibration of a Parallel Kinematic Machine Tool

    Institute of Scientific and Technical Information of China (English)

    HE Xiao-mei; DING Hong-sheng; FU Tie; XIE Dian-huang; XU Jin-zhong; LI Hua-feng; LIU Hui-lin

    2006-01-01

    A calibration method is presented to enhance the static accuracy of a parallel kinematic machine tool by using a coordinate measuring machine and a laser tracker. According to the established calibration model and the calibration experiment, the factual 42 kinematic parameters of BKX-I parallel kinematic machine tool are obtained. By circular tests the comparison is made between the calibrated and the uncalibrated parameters and shows that there is 80% improvement in accuracy of this machine tool.

  1. Binary Classifier Calibration: Non-parametric approach

    OpenAIRE

    Naeini, Mahdi Pakdaman; Cooper, Gregory F.; Hauskrecht, Milos

    2014-01-01

    Accurate calibration of probabilistic predictive models learned is critical for many practical prediction and decision-making tasks. There are two main categories of methods for building calibrated classifiers. One approach is to develop methods for learning probabilistic models that are well-calibrated, ab initio. The other approach is to use some post-processing methods for transforming the output of a classifier to be well calibrated, as for example histogram binning, Platt scaling, and is...

  2. GREAT/SOFIA atmospheric calibration

    OpenAIRE

    Guan, Xin; Stutzki, Jürgen; Graf, Urs U.; Güsten, Rolf; Okada, Yoko; Torres, Miguel Angel Requena; Simon, Robert; Wiesemeyer, Helmut

    2012-01-01

    The GREAT observations need frequency-selective calibration across the passband for the residual atmospheric opacity at flight altitude. At these altitudes the atmospheric opacity has both narrow and broad spectral features. To determine the atmospheric transmission at high spectral resolution, GREAT compares the observed atmospheric emission with atmospheric model predictions, and therefore depends on the validity of the atmospheric models. We discusse the problems identified in this compari...

  3. Calibrating thermal behavior of electronics

    Science.gov (United States)

    Chainer, Timothy J.; Parida, Pritish R.; Schultz, Mark D.

    2016-05-31

    A method includes determining a relationship between indirect thermal data for a processor and a measured temperature associated with the processor, during a calibration process, obtaining the indirect thermal data for the processor during actual operation of the processor, and determining an actual significant temperature associated with the processor during the actual operation using the indirect thermal data for the processor during actual operation of the processor and the relationship.

  4. Los Alamos second-generation system for passive and active neutron assays of drum-size containers

    International Nuclear Information System (INIS)

    We describe in a comprehensive fashion the Los Alamos second-generation system for passive and active neutron assays of drum-size containers. The developmental history of this 7-year project is presented with emphasis on the pulsed active neutron technique (differential dieaway), which has achieved milligram levels of assay sensitivity for both plutonium and uranium wastes. We describe in detail the matrix effects for both passive and active neutron assays. We present in a thorough fashion our novel approach to achieving comprehensive corrections for these matrix effects using measurements made during the assays. We develop a matrix correction formalism based on separate neutron absorption and moderator indices determined from these measurements. These are presented as a series of analytic functions fitted to the data. Absolute calibrations and calibration standards are discussed, as is a practical means (pink drum measurements) of achieving routine calibration verification at all implementation sites. We present our overall assay algorithm, integrating absolute calibrations with matrix corrections. We also present a systematic error formalism that is based on the matrix response data. Finally, we outline a strategy for the verification of our entire assay formalism. This is based on measurements with a set of salted waste matrix drums combined with systematic assay intercomparisons of well-characterized transuranic wastes

  5. Unassisted 3D camera calibration

    Science.gov (United States)

    Atanassov, Kalin; Ramachandra, Vikas; Nash, James; Goma, Sergio R.

    2012-03-01

    With the rapid growth of 3D technology, 3D image capture has become a critical part of the 3D feature set on mobile phones. 3D image quality is affected by the scene geometry as well as on-the-device processing. An automatic 3D system usually assumes known camera poses accomplished by factory calibration using a special chart. In real life settings, pose parameters estimated by factory calibration can be negatively impacted by movements of the lens barrel due to shaking, focusing, or camera drop. If any of these factors displaces the optical axes of either or both cameras, vertical disparity might exceed the maximum tolerable margin and the 3D user may experience eye strain or headaches. To make 3D capture more practical, one needs to consider unassisted (on arbitrary scenes) calibration. In this paper, we propose an algorithm that relies on detection and matching of keypoints between left and right images. Frames containing erroneous matches, along with frames with insufficiently rich keypoint constellations, are detected and discarded. Roll, pitch yaw , and scale differences between left and right frames are then estimated. The algorithm performance is evaluated in terms of the remaining vertical disparity as compared to the maximum tolerable vertical disparity.

  6. Model Calibration in Watershed Hydrology

    Science.gov (United States)

    Yilmaz, Koray K.; Vrugt, Jasper A.; Gupta, Hoshin V.; Sorooshian, Soroosh

    2009-01-01

    Hydrologic models use relatively simple mathematical equations to conceptualize and aggregate the complex, spatially distributed, and highly interrelated water, energy, and vegetation processes in a watershed. A consequence of process aggregation is that the model parameters often do not represent directly measurable entities and must, therefore, be estimated using measurements of the system inputs and outputs. During this process, known as model calibration, the parameters are adjusted so that the behavior of the model approximates, as closely and consistently as possible, the observed response of the hydrologic system over some historical period of time. This Chapter reviews the current state-of-the-art of model calibration in watershed hydrology with special emphasis on our own contributions in the last few decades. We discuss the historical background that has led to current perspectives, and review different approaches for manual and automatic single- and multi-objective parameter estimation. In particular, we highlight the recent developments in the calibration of distributed hydrologic models using parameter dimensionality reduction sampling, parameter regularization and parallel computing.

  7. PACS photometer calibration block analysis

    CERN Document Server

    Moór, A; Kiss, Cs; Balog, Z; Billot, N; Marton, G

    2013-01-01

    The absolute stability of the PACS bolometer response over the entire mission lifetime without applying any corrections is about 0.5% (standard deviation) or about 8% peak-to-peak. This fantastic stability allows us to calibrate all scientific measurements by a fixed and time-independent response file, without using any information from the PACS internal calibration sources. However, the analysis of calibration block observations revealed clear correlations of the internal source signals with the evaporator temperature and a signal drift during the first half hour after the cooler recycling. These effects are small, but can be seen in repeated measurements of standard stars. From our analysis we established corrections for both effects which push the stability of the PACS bolometer response to about 0.2% (stdev) or 2% in the blue, 3% in the green and 5% in the red channel (peak-to-peak). After both corrections we still see a correlation of the signals with PACS FPU temperatures, possibly caused by parasitic h...

  8. New method for calibration of sun photometers

    Institute of Scientific and Technical Information of China (English)

    H.H.Asadov; I.G.Chobanzadeh

    2009-01-01

    A new method for calibration of sun photometers based on Bouguer-Beer law is proposed.The developed basic equation of calibration makes it possible to formulate the derivative methods of calibration on the basis of photometric measurements upon optical air masses,the ratio of which is an integer number.

  9. 48 CFR 908.7113 - Calibration services.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Calibration services. 908... ACQUISITION PLANNING REQUIRED SOURCES OF SUPPLIES AND SERVICES Acquisition of Special Items 908.7113 Calibration services. Orders for calibration services may be placed with the National Institute of...

  10. Net analyte signal calculation for multivariate calibration

    NARCIS (Netherlands)

    Ferre, J.; Faber, N.M.

    2003-01-01

    A unifying framework for calibration and prediction in multivariate calibration is shown based on the concept of the net analyte signal (NAS). From this perspective, the calibration step can be regarded as the calculation of a net sensitivity vector, whose length is the amount of net signal when the

  11. Isolation of Potent CGRP Neutralizing Antibodies Using Four Simple Assays.

    Science.gov (United States)

    Neal, Frances; Arnold, Joanne; Rossant, Christine J; Podichetty, Sadhana; Lowne, David; Dobson, Claire; Wilkinson, Trevor; Colley, Caroline; Howes, Rob; Vaughan, Tristan J

    2016-01-01

    Calcitonin gene-related peptide (CGRP) is a small neuropeptide and a potent vasodilator that is widely associated with chronic pain and migraine. An antibody that inhibits CGRP function would be a potential therapeutic for treatment of these disorders. Here we describe the isolation of highly potent antibodies to CGRP from phage and ribosome display libraries and characterization of their epitope, species cross-reactivity, kinetics, and functional activity. Homogenous time-resolved fluorescence (HTRF) binding assays identified antibodies with the desired species cross-reactivity from naïve libraries, and HTRF epitope competition assays were used to characterize and group scFv by epitope. The functional inhibition of CGRP and species cross-reactivity of purified scFv and antibodies were subsequently confirmed using cAMP assays. We show that epitope competition assays could be used as a surrogate for functional cell-based assays during affinity maturation, in combination with scFv off-rate ranking by biolayer interferometry (BLI). This is the first time it has been shown that off-rate ranking can be predictive of functional activity for anti-CGRP antibodies. Here we demonstrate how, by using just four simple assays, diverse panels of antibodies to CGRP can be identified. These assay formats have potential utility in the identification of antibodies to other therapeutic targets. PMID:26450103

  12. Comparison of RIA, IRMA and LIA assays of serum ferritin

    International Nuclear Information System (INIS)

    The results of RIA, IRMA and LIA assays of ferritin in the human serum were compared. Various kits: IRMA FARMOS SPECTRIA (Finland), IRMA (CIS), the non-kit method RIA based on human placental ferritin prepared by the authors, and LIA-AMERLITE AMERSHAM (England) were evaluated and compared with RIA AMERSHAM (England). It was found that IRMA FARMOS SPECTRIA and the non-kit RIA assay based on placental ferritin give comparable and lower results in the whole range of the calibration curve (0-1000 μg/l) of RIA Amersham: 10-15% lower for the non-kit assay and 15-20% lower for the IRMA assay. The IRMA assay (CIS) gives comparable and higher results in the range up to 300 μg/l. The results for more concentrated solutions of ferritin with above 350 μg/l are low and unreliable. Correlation is not linear. Amerlite, a non-isotopic method, gives results comparable with the RIA method as far as accuracy and correctness is concerned. The range of the calibration curve is 0-2000 μg/l. The values of ferritin in serum are somewhat lower in the range up to 100 μg/l and the same values are somewhat higher in the range above 100 μg/l when comapred with the commercial RIA kit of Amersham. Altogether the values measured are within the range of statistical variance of values measured by the RIA Amersham method. (author) 4 tabs., 3 figs., 4 refs

  13. Practical assay issues with the PERT/PBRT assay: a highly sensitive reverse transcriptase assay.

    Science.gov (United States)

    Chang, A; Dusing, S

    2006-01-01

    Product safety testing for retroviruses can be achieved by a panel of screening assays, including electron microscopy, viral gene specific PCRs, virus propagation, and detection of reverse transciptase activity. The application of PCR-based reverse transcriptase assays (PERT) that are approximately a million-fold more sensitive than conventional nucleotide incorporation assays in the testing of biologicals is described. Use of PERT assays can be applied to three areas: (i) screening for adventitious retrovirus contamination; (ii) detecting and quantifying endogenous viral particle load and (iii) monitoring levels of infectious retrovirus generation in cell lines that contain endogenous retroviruses.

  14. Muon Energy Calibration of the MINOS Detectors

    Energy Technology Data Exchange (ETDEWEB)

    Miyagawa, Paul S.

    2004-09-01

    MINOS is a long-baseline neutrino oscillation experiment designed to search for conclusive evidence of neutrino oscillations and to measure the oscillation parameters precisely. MINOS comprises two iron tracking calorimeters located at Fermilab and Soudan. The Calibration Detector at CERN is a third MINOS detector used as part of the detector response calibration programme. A correct energy calibration between these detectors is crucial for the accurate measurement of oscillation parameters. This thesis presents a calibration developed to produce a uniform response within a detector using cosmic muons. Reconstruction of tracks in cosmic ray data is discussed. This data is utilized to calculate calibration constants for each readout channel of the Calibration Detector. These constants have an average statistical error of 1.8%. The consistency of the constants is demonstrated both within a single run and between runs separated by a few days. Results are presented from applying the calibration to test beam particles measured by the Calibration Detector. The responses are calibrated to within 1.8% systematic error. The potential impact of the calibration on the measurement of oscillation parameters by MINOS is also investigated. Applying the calibration reduces the errors in the measured parameters by {approx} 10%, which is equivalent to increasing the amount of data by 20%.

  15. "Calibration-on-the-spot": How to calibrate an EMCCD camera from its images.

    Science.gov (United States)

    Mortensen, Kim I; Flyvbjerg, Henrik

    2016-07-06

    In order to count photons with a camera, the camera must be calibrated. Photon counting is necessary, e.g., to determine the precision of localization-based super-resolution microscopy. Here we present a protocol that calibrates an EMCCD camera from information contained in isolated, diffraction-limited spots in any image taken by the camera, thus making dedicated calibration procedures redundant by enabling calibration post festum, from images filed without calibration information.

  16. Calibration-on-the-spot”: How to calibrate an EMCCD camera from its images

    DEFF Research Database (Denmark)

    Mortensen, Kim; Flyvbjerg, Henrik

    2016-01-01

    In order to count photons with a camera, the camera must be calibrated. Photon counting is necessary, e.g., to determine the precision of localization-based super-resolution microscopy. Here we present a protocol that calibrates an EMCCD camera from information contained in isolated, diffraction......-limited spots in any image taken by the camera, thus making dedicated calibration procedures redundant by enabling calibration post festum, from images filed without calibration information....

  17. Calibration of the Cherenkov Telescope Array

    CERN Document Server

    Gaug, Markus; Berge, David; Reyes, Raquel de los; Doro, Michele; Foerster, Andreas; Maccarone, Maria Concetta; Parsons, Dan; van Eldik, Christopher

    2015-01-01

    The construction of the Cherenkov Telescope Array is expected to start soon. We will present the baseline methods and their extensions currently foreseen to calibrate the observatory. These are bound to achieve the strong requirements on allowed systematic uncertainties for the reconstructed gamma-ray energy and flux scales, as well as on the pointing resolution, and on the overall duty cycle of the observatory. Onsite calibration activities are designed to include a robust and efficient calibration of the telescope cameras, and various methods and instruments to achieve calibration of the overall optical throughput of each telescope, leading to both inter-telescope calibration and an absolute calibration of the entire observatory. One important aspect of the onsite calibration is a correct understanding of the atmosphere above the telescopes, which constitutes the calorimeter of this detection technique. It is planned to be constantly monitored with state-of-the-art instruments to obtain a full molecular and...

  18. Multi-Axis Accelerometer Calibration System

    Science.gov (United States)

    Finley, Tom; Parker, Peter

    2010-01-01

    A low-cost, portable, and simplified system has been developed that is suitable for in-situ calibration and/or evaluation of multi-axis inertial measurement instruments. This system overcomes facility restrictions and maintains or improves the calibration quality for users of accelerometer-based instruments with applications in avionics, experimental wind tunnel research, and force balance calibration applications. The apparatus quickly and easily positions a multi-axis accelerometer system into a precisely known orientation suitable for in-situ quality checks and calibration. In addition, the system incorporates powerful and sophisticated statistical methods, known as response surface methodology and statistical quality control. These methods improve calibration quality, reduce calibration time, and allow for increased calibration frequency, which enables the monitoring of instrument stability over time.

  19. MODIS Instrument Operation and Calibration Improvements

    Science.gov (United States)

    Xiong, X.; Angal, A.; Madhavan, S.; Link, D.; Geng, X.; Wenny, B.; Wu, A.; Chen, H.; Salomonson, V.

    2014-01-01

    Terra and Aqua MODIS have successfully operated for over 14 and 12 years since their respective launches in 1999 and 2002. The MODIS on-orbit calibration is performed using a set of on-board calibrators, which include a solar diffuser for calibrating the reflective solar bands (RSB) and a blackbody for the thermal emissive bands (TEB). On-orbit changes in the sensor responses as well as key performance parameters are monitored using the measurements of these on-board calibrators. This paper provides an overview of MODIS on-orbit operation and calibration activities, and instrument long-term performance. It presents a brief summary of the calibration enhancements made in the latest MODIS data collection 6 (C6). Future improvements in the MODIS calibration and their potential applications to the S-NPP VIIRS are also discussed.

  20. Training In Diversity Management

    Directory of Open Access Journals (Sweden)

    Sonja Treven

    2011-07-01

    Full Text Available The labor force all around the world is becoming increasingly diverse. Thus, organizations that can manage employee diversity effectively gain a competitive advantage. In such organizations diversity training is a necessity. Diversity training helps managers understand and value individual differences and develop strong diagnostic skills. The paper explores various approaches to training, like awareness-based and skill-based diversity training. A special attention to potential problems that may occur in the process of diversity training is given.

  1. Diversity: A Philosophical Perspective

    OpenAIRE

    Sahotra Sarkar

    2010-01-01

    In recent years, diversity, whether it be ecological, biological, cultural, or linguistic diversity, has emerged as a major cultural value. This paper analyzes whether a single concept of diversity can underwrite discussions of diversity in different disciplines. More importantly, it analyzes the normative justification for the endorsement of diversity as a goal in all contexts. It concludes that no more than a relatively trivial concept of diversity as richness is common to all contexts. Mor...

  2. Diversity: A Philosophical Perspective

    Directory of Open Access Journals (Sweden)

    Sahotra Sarkar

    2010-01-01

    Full Text Available In recent years, diversity, whether it be ecological, biological, cultural, or linguistic diversity, has emerged as a major cultural value. This paper analyzes whether a single concept of diversity can underwrite discussions of diversity in different disciplines. More importantly, it analyzes the normative justification for the endorsement of diversity as a goal in all contexts. It concludes that no more than a relatively trivial concept of diversity as richness is common to all contexts. Moreover, there is no universal justification for the endorsement of diversity. Arguments to justify the protection of diversity must be tailored to individual contexts.

  3. Protocols for calibrating multibeam sonar

    OpenAIRE

    Foote, Kenneth G.; Chu, Dezhang; Hammar, Terence R.; Baldwin, Kenneth C.; Mayer, Larry A.; Hufnagle, Lawrence C. jr.; Jech, J. Michael

    2005-01-01

    Development of protocols for calibrating multibeam sonar by means of the standard-target method is documented. Particular systems used in the development work included three that provide the water-column signals, namely the SIMRAD SM2000/90- and 200-kHz sonars and RESON SeaBat 8101 sonar, with operating frequency of 240 kHz. Two facilities were instrumented specifically for the work: a sea well at the Woods Hole Oceanographic Institution and a large, indoor freshwater tank at the University o...

  4. Calibration of TOB+ Thermometer's Cards

    CERN Document Server

    Banitt, Daniel

    2014-01-01

    Motivation - Under the new upgrade of the CMS detector the working temperature of the trackers had been reduced to -27 Celsius degrees. Though the thermal sensors themselves (Murata and Fenwal thermistors) are effective at these temperatures, the max1542 PLC (programmable logic controller) cards, interpreting the resistance of the thermal sensors into DC counts usable by the DCS (detector control system), are not designed for these temperatures in which the counts exceed their saturation and therefor had to be replaced. In my project I was in charge of handling the emplacement and calibration of the new PLC cards to the TOB (tracker outer barrel) control system.

  5. From Antenna to Assay

    Science.gov (United States)

    Moore, Evan G.; Samuel, Amanda P. S.; Raymond, Kenneth N.

    2009-01-01

    Conspectus Ligand-sensitized, luminescent lanthanide(III) complexes are of considerable importance because their unique photophysical properties (microsecond to millisecond lifetimes, characteristic and narrow emission bands, and large Stokes shifts) make them well suited as labels in fluorescence-based bioassays. The long-lived emission of lanthanide(III) cations can be temporally resolved from scattered light and background fluorescence to vastly enhance measurement sensitivity. One challenge in this field is the design of sensitizing ligands that provide highly emissive complexes with sufficient stability and aqueous solubility for practical applications. In this Account, we give an overview of some of the general properties of the trivalent lanthanides and follow with a summary of advances made in our laboratory in the development of highly luminescent Tb(III) and Eu(III) complexes for applications in biotechnology. A focus of our research has been the optimization of these compounds as potential commercial agents for use in Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our approach involves developing high-stability octadentate Tb(III) and Eu(III) complexes that rely on all-oxygen donor atoms and using multi-chromophore chelates to increase molar absorptivity; earlier examples utilized a single pendant chromophore (that is, a single “antenna”). Ligands based on 2-hydroxyisophthalamide (IAM) provide exceptionally emissive Tb(III) complexes with quantum yield values up to ∼60% that are stable at the nanomolar concentrations required for commercial assays. Through synthetic modification of the IAM chromophore and time-dependent density functional theory (TD-DFT) calculations, we have developed a method to predict absorption and emission properties of these chromophores as a tool to guide ligand design. Additionally, we have investigated chiral IAM ligands that yield Tb(III) complexes possessing both high quantum yield values and strong

  6. Translating pharmacodynamic biomarkers from bench to bedside: analytical validation and fit-for-purpose studies to qualify multiplex immunofluorescent assays for use on clinical core biopsy specimens.

    Science.gov (United States)

    Marrero, Allison; Lawrence, Scott; Wilsker, Deborah; Voth, Andrea Regier; Kinders, Robert J

    2016-08-01

    Multiplex pharmacodynamic (PD) assays have the potential to increase sensitivity of biomarker-based reporting for new targeted agents, as well as revealing significantly more information about target and pathway activation than single-biomarker PD assays. Stringent methodology is required to ensure reliable and reproducible results. Common to all PD assays is the importance of reagent validation, assay and instrument calibration, and the determination of suitable response calibrators; however, multiplex assays, particularly those performed on paraffin specimens from tissue blocks, bring format-specific challenges adding a layer of complexity to assay development. We discuss existing multiplex approaches and the development of a multiplex immunofluorescence assay measuring DNA damage and DNA repair enzymes in response to anti-cancer therapeutics and describe how our novel method addresses known issues. PMID:27663477

  7. Pulse-based internal calibration of polarimetric SAR

    DEFF Research Database (Denmark)

    Dall, Jørgen; Skou, Niels; Christensen, Erik Lintz

    1994-01-01

    Internal calibration greatly diminishes the dependence on calibration target deployment compared to external calibration. Therefore the Electromagnetics Institute (EMI) at the Technical University of Denmark (TUD) has equipped its polarimetric SAR, EMISAR, with several calibration loops and devel......Internal calibration greatly diminishes the dependence on calibration target deployment compared to external calibration. Therefore the Electromagnetics Institute (EMI) at the Technical University of Denmark (TUD) has equipped its polarimetric SAR, EMISAR, with several calibration loops...

  8. CALIBRATION OF SEISMIC ATTRIBUTES FOR RESERVOIR CHARACTERIZATION

    Energy Technology Data Exchange (ETDEWEB)

    Wayne D. Pennington

    2001-04-01

    The project, ''Calibration of Seismic Attributes for Reservoir Calibration,'' is on schedule and making unplanned discoveries in addition to those intended when the project commenced. The discoveries, planned and unplanned, can be grouped into four classes: pitfalls to avoid in interpretation of seismic attributes; suggested workflows to follow in working with seismic attributes; new methods of calculating certain new attributes which we feel to be useful; and new theoretical approaches to certain petrophysical properties. We are using data from Wyoming, North Texas, South Texas, and the Gulf of Mexico offshore of Louisiana. These environments provide a diverse array of physical conditions and rock types, and a variety of interpretation methods to be applied to them. The Wyoming field is a very difficult one, including alternating layers of thin beds of coals, shales, and hard sandstones, and there may be an observable effect due to hydrocarbon production; we are using this field as the ''test'' of those techniques and methods we have developed or that we prefer based on our work on the other fields. Work on this field is still underway, although progressing nicely. The work on the public domain data sets in Texas, Boonsville and Stratton, is complete except for some minor additional processing steps, and final write-ups are underway. The work on the Gulf of Mexico field has been completed to the extent originally planned, but it has led us to such important new observations and discoveries that we have expanded our original scope to include time-lapse studies and petrophysical aspects of pressure changes; work on this expanded scope is continuing. Presentations have been made at professional-society meetings, company offices, consortium workshops, and university settings. Papers, including one review paper on ''Reservoir Geophysics'' have been published. Several Master's theses, which will spin off

  9. New Application of the Comet Assay: Chromosome–Comet Assay

    OpenAIRE

    Cortés-Gutiérrez, Elva I.; DÁVILA-RODRÍGUEZ, MARTHA I.; Fernández, José Luís; López-Fernández, Carmen; Gosálbez, Altea; Gosálvez, Jaime

    2011-01-01

    The comet assay is a well-established, simple, versatile, visual, rapid, and sensitive tool used extensively to assess DNA damage and DNA repair quantitatively and qualitatively in single cells. The comet assay is most frequently used to analyze white blood cells or lymphocytes in human biomonitoring studies, although other cell types have been examined, including buccal, nasal, epithelial, and placental cells and even spermatozoa. This study was conducted to design a protocol that can be use...

  10. MODIS solar reflective calibration traceability

    Science.gov (United States)

    Xiong, Xiaoxiong; Butler, Jim

    2009-08-01

    Long-term climate data records often consist of observations made by multiple sensors. It is, therefore, extremely important to have instrument overlap, to be able to track instrument stability, to quantify measurement uncertainties, and to establish an absolute measurement scale traceable to the International System of Units (SI). The Moderate Resolution Imaging Spectroradiometer (MODIS) is a key instrument for both the Terra and Aqua missions, which were launched in December 1999 and May 2002, respectively. It has 20 reflective solar bands (RSB) with wavelengths from 0.41 to 2.2μm and observes the Earth at three nadir spatial resolutions: 0.25km, 0.5km, and 1km. MODIS RSB on-orbit calibration is reflectance based with reference to the bi-directional reflectance factor (BRF) of its on-board solar diffuser (SD). The SD BRF characterization was made pre-launch by the instrument vendor using reference samples traceable directly to the National Institute of Standards and Technology (NIST). On-orbit SD reflectance degradation is tracked by an on-board solar diffuser stability monitor (SDSM). This paper provides details of this calibration chain, from pre-launch to on-orbit operation, and associated uncertainty assessments. Using MODIS as an example, this paper also discusses challenges and key design requirements for future missions developed for accurate climate studies.

  11. Crop physiology calibration in CLM

    Directory of Open Access Journals (Sweden)

    I. Bilionis

    2014-10-01

    Full Text Available Farming is using more terrestrial ground, as population increases and agriculture is increasingly used for non-nutritional purposes such as biofuel production. This agricultural expansion exerts an increasing impact on the terrestrial carbon cycle. In order to understand the impact of such processes, the Community Land Model (CLM has been augmented with a CLM-Crop extension that simulates the development of three crop types: maize, soybean, and spring wheat. The CLM-Crop model is a complex system that relies on a suite of parametric inputs that govern plant growth under a given atmospheric forcing and available resources. CLM-Crop development used measurements of gross primary productivity and net ecosystem exchange from AmeriFlux sites to choose parameter values that optimize crop productivity in the model. In this paper we calibrate these parameters for one crop type, soybean, in order to provide a faithful projection in terms of both plant development and net carbon exchange. Calibration is performed in a Bayesian framework by developing a scalable and adaptive scheme based on sequential Monte Carlo (SMC.

  12. MODIS Solar Reflective Calibration Traceability

    Science.gov (United States)

    Xiong, Xiaoxiong; Butler, Jim

    2009-01-01

    Long-term climate data records often consist of observations made by multiple sensors. It is, therefore, extremely important to have instrument overlap, to be able to track instrument stability, to quantify, measurement uncertainties, and to establish absolute scale traceable to the International System of Units (SI). The Moderate Resolution Imaging Spectroradiometer (MODIS) is a key instrument for both the Terra and Aqua missions, which were launched in December 1999 and May 2002, respectively. It has 20 reflective solar bands (RSB) with wavelengths from 0.41 to 2.2 micrometers and observes the Earth at three nadir spatial resolutions: 0.25km, 0.5km, and 1km. MODIS RSB on-orbit calibration is reflectance based with reference to the bidirectional reflectance factor (BRF) of its on-board solar diffuser (SD). The SD BRF characterization was made pre-launch by the instrument vendor using reference samples traceable directly to the National Institute of Standards and Technology (NIST). On-orbit SD reflectance degradation is tracked by an on-board solar diffuser monitor (SDSM). This paper provides details of this calibration chain, from prelaunch to on-orbit operation, and associated uncertainty assessments. Using MODIS as an example, this paper also discusses challenges and key design requirements for future missions developed for accurate climate studies.

  13. Calibration and rectification research for fish-eye lens application

    Science.gov (United States)

    Feng, Weijia; Zhang, Baofeng; Cao, Zuoliang; Zong, Xiaoning; Röning, Juha

    2011-01-01

    The purpose of this paper aims to promote the application of fish-eye lens. Accurate parameters calibration and effective distortion rectification of an imaging device is of utmost importance in machine vision. Fish-eye lens produces a hemispherical field of view of an environment, which appears definite significant since its advantage of panoramic sight with a single compact visual scene. But fish-eye lens image has an unavoidable inherent severe distortion. The precise optical center is the precondition for other parameters calibration and distortion correction. Therefore, three different optical center calibration methods have been researched for diverse applications. Support Vector Machine (SVM) and Spherical Equidistance Projection Algorithm (SEPA) are integrated to replace traditional rectification methods. SVM is a machine learning method based on the theory of statistics, which have good capabilities of imitating, regression and classification. In this research, SVM provides a mapping table between the fish-eye image and the standard image for human eyes. Two novel training models have been designed. SEPA has been applied to promote the rectification effect of the edge of fish-eye lens image. The validity and effectiveness of our achievements are demonstrated by processing the real images.

  14. Comparison of chemosensitivity tests: clonogenic assay versus MTT assay.

    Directory of Open Access Journals (Sweden)

    Kawada K

    2002-06-01

    Full Text Available When the development of chemotherapeutic agents reaches the clinical trial stage, it is necessary to perform drug sensitivity tests quickly in order to select the most promising agents for the treatment of cancer. In order to assess the possibility of using the 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT assay as a substitute for the human tumor clonogenic assay (HTCA, we evaluated the correlation between the results obtained by these 2 assays in 5 human lung cancer cell lines. The correlation coefficient between the results of the HTCA and the MTT assay was 0.673, indicating a relatively good correlation. The correlation was most prominent in platinum analogues (r = 0.939 and good in anthracyclines/anthracenedione (r = 0.611. However, no significant correlation was observed in vinca alkaloids, etoposide, irinotecan, SN-38 (an active metabolite of irinotecan, and rhizoxin. The results of the MTT assay showed a high degree of correlation with those of the HTCA in predicting the sensitivity of cancer cell lines to platinum analogues, and anthracyclines/anthracenedione. These results suggest that the MTT assay may be more convenient and quickly performed than the HTCA and can replace HTCA in evaluating the effects of anticancer agents, especially the platinum analogues and anthracyclines/anthracenedione.

  15. Calorimetric assay of minor actinides

    Energy Technology Data Exchange (ETDEWEB)

    Rudy, C.; Bracken, D.; Cremers, T.; Foster, L.A.; Ensslin, N.

    1996-12-31

    This paper reviews the principles of calorimetric assay and evaluates its potential application to the minor actinides (U-232-4, Am-241, Am- 243, Cm-245, Np-237). We conclude that calorimetry and high- resolution gamma-ray isotopic analysis can be used for the assay of minor actinides by adapting existing methodologies for Pu/Am-241 mixtures. In some cases, mixtures of special nuclear materials and minor actinides may require the development of new methodologies that involve a combination of destructive and nondestructive assay techniques.

  16. Calorimetric assay of minor actinides

    International Nuclear Information System (INIS)

    This paper reviews the principles of calorimetric assay and evaluates its potential application to the minor actinides (U-232-4, Am-241, Am- 243, Cm-245, Np-237). We conclude that calorimetry and high- resolution gamma-ray isotopic analysis can be used for the assay of minor actinides by adapting existing methodologies for Pu/Am-241 mixtures. In some cases, mixtures of special nuclear materials and minor actinides may require the development of new methodologies that involve a combination of destructive and nondestructive assay techniques

  17. HPGe Detector Efficiency Calibration Using HEU Standards

    Energy Technology Data Exchange (ETDEWEB)

    Salaymeh, S.R.

    2000-10-12

    The Analytical Development Section of SRTC was requested by the Facilities Disposition Division (FDD) to determine the holdup of enriched uranium in the 321-M facility as part of an overall deactivation project of the facility. The 321-M facility was used to fabricate enriched uranium fuel assemblies, lithium-aluminum target tubes, neptunium assemblies, and miscellaneous components for the production reactors. The facility also includes the 324-M storage building and the passageway connecting it to 321-M. The results of the holdup assays are essential for determining compliance with the Solid Waste's Waste Acceptance Criteria, Material Control and Accountability, and to meet criticality safety controls. Two measurement systems will be used to determine highly enriched uranium (HEU) holdup: One is a portable HPGe detector and EG and G Dart system that contains high voltage power supply and signal processing electronics. A personal computer with Gamma-Vision software was used to provide an MCA card, and space to store and manipulate multiple 4096-channel g-ray spectra. The other is a 2 inches x 2 inches NaI crystal with an MCA that uses a portable computer with a Canberra NaI plus card installed. This card converts the PC to a full function MCA and contains the ancillary electronics, high voltage power supply and amplifier, required for data acquisition. This report describes and documents the HPGe point, line, area, and constant geometry-constant transmission detector efficiency calibrations acquired and calculated for use in conducting holdup measurements as part of the overall deactivation project of building 321-M.

  18. Another look at volume self-calibration: calibration and self-calibration within a pinhole model of Scheimpflug cameras

    Science.gov (United States)

    Cornic, Philippe; Illoul, Cédric; Cheminet, Adam; Le Besnerais, Guy; Champagnat, Frédéric; Le Sant, Yves; Leclaire, Benjamin

    2016-09-01

    We address calibration and self-calibration of tomographic PIV experiments within a pinhole model of cameras. A complete and explicit pinhole model of a camera equipped with a 2-tilt angles Scheimpflug adapter is presented. It is then used in a calibration procedure based on a freely moving calibration plate. While the resulting calibrations are accurate enough for Tomo-PIV, we confirm, through a simple experiment, that they are not stable in time, and illustrate how the pinhole framework can be used to provide a quantitative evaluation of geometrical drifts in the setup. We propose an original self-calibration method based on global optimization of the extrinsic parameters of the pinhole model. These methods are successfully applied to the tomographic PIV of an air jet experiment. An unexpected by-product of our work is to show that volume self-calibration induces a change in the world frame coordinates. Provided the calibration drift is small, as generally observed in PIV, the bias on the estimated velocity field is negligible but the absolute location cannot be accurately recovered using standard calibration data.

  19. Real‑time, fast neutron detection for stimulated safeguards assay

    International Nuclear Information System (INIS)

    The advent of low‑hazard organic liquid scintillation detectors and real‑time pulse‑shape discrimination (PSD) processing has suggested a variety of modalities by which fast neutrons, as opposed to neutrons moderated prior to detection, can be used directly to benefit safeguards needs. In this paper we describe a development of a fast‑neutron based safeguards assay system designed for the assessment of 235U content in fresh fuel. The system benefits from real‑time pulse‑shape discrimination processing and auto‑calibration of the detector system parameters to ensure a rapid and effective set‑up protocol. These requirements are essential in optimising the speed and limit of detection of the fast neutron technique, whilst minimising the intervention needed to perform the assay.

  20. Research of Camera Calibration Based on DSP

    Directory of Open Access Journals (Sweden)

    Zheng Zhang

    2013-09-01

    Full Text Available To take advantage of the high-efficiency and stability of DSP in the data processing and the functions of OpenCV library, this study brought forward a scheme that camera calibration in DSP embedded system calibration. An arithmetic of camera calibration based on OpenCV is designed by analyzing the camera model and lens distortion. The transplantation of EMCV to DSP is completed and the arithmetic of camera calibration is migrated and optimized based on the CCS development environment and the DSP/BIOS system. On the premise of realizing calibration function, this arithmetic improves the efficiency of program execution and the precision of calibration and lays the foundation for further research of the visual location based on DSP embedded system.

  1. Absolute calibration in vivo measurement systems

    International Nuclear Information System (INIS)

    Lawrence Livermore National Laboratory (LLNL) is currently investigating a new method for obtaining absolute calibration factors for radiation measurement systems used to measure internally deposited radionuclides in vivo. Absolute calibration of in vivo measurement systems will eliminate the need to generate a series of human surrogate structures (i.e., phantoms) for calibrating in vivo measurement systems. The absolute calibration of in vivo measurement systems utilizes magnetic resonance imaging (MRI) to define physiological structure, size, and composition. The MRI image provides a digitized representation of the physiological structure, which allows for any mathematical distribution of radionuclides within the body. Using Monte Carlo transport codes, the emission spectrum from the body is predicted. The in vivo measurement equipment is calibrated using the Monte Carlo code and adjusting for the intrinsic properties of the detection system. The calibration factors are verified using measurements of existing phantoms and previously obtained measurements of human volunteers. 8 refs

  2. Herschel SPIRE FTS Relative Spectral Response Calibration

    CERN Document Server

    Fulton, Trevor; Baluteau, Jean-Paul; Benielli, Dominique; Imhof, Peter; Lim, Tanya; Lu, Nanyao; Marchili, Nicola; Naylor, David; Polehampton, Edward; Swinyard, Bruce; Valtchanov, Ivan

    2014-01-01

    Herschel/SPIRE Fourier transform spectrometer (FTS) observations contain emission from both the Herschel Telescope and the SPIRE Instrument itself, both of which are typically orders of magnitude greater than the emission from the astronomical source, and must be removed in order to recover the source spectrum. The effects of the Herschel Telescope and the SPIRE Instrument are removed during data reduction using relative spectral response calibration curves and emission models. We present the evolution of the methods used to derive the relative spectral response calibration curves for the SPIRE FTS. The relationship between the calibration curves and the ultimate sensitivity of calibrated SPIRE FTS data is discussed and the results from the derivation methods are compared. These comparisons show that the latest derivation methods result in calibration curves that impart a factor of between 2 and 100 less noise to the overall error budget, which results in calibrated spectra for individual observations whose n...

  3. Biogeographic calibrations for the molecular clock.

    Science.gov (United States)

    Ho, Simon Y W; Tong, K Jun; Foster, Charles S P; Ritchie, Andrew M; Lo, Nathan; Crisp, Michael D

    2015-09-01

    Molecular estimates of evolutionary timescales have an important role in a range of biological studies. Such estimates can be made using methods based on molecular clocks, including models that are able to account for rate variation across lineages. All clock models share a dependence on calibrations, which enable estimates to be given in absolute time units. There are many available methods for incorporating fossil calibrations, but geological and climatic data can also provide useful calibrations for molecular clocks. However, a number of strong assumptions need to be made when using these biogeographic calibrations, leading to wide variation in their reliability and precision. In this review, we describe the nature of biogeographic calibrations and the assumptions that they involve. We present an overview of the different geological and climatic events that can provide informative calibrations, and explain how such temporal information can be incorporated into dating analyses.

  4. Calibration biases in logical reasoning tasks

    OpenAIRE

    Guillermo Macbeth; Alfredo López Alonso; Eugenia Razumiejczyk; Rodrigo Sosa; Carolina Pereyra; Humberto Fernández

    2013-01-01

    The aim of this contribution is to present an experimental study about calibration in deductive reasoning tasks. Calibration is defi ned as the empirical convergence or divergence between the objective and the subjective success. The underconfi dence bias is understood as the dominance of the former over the latter. The hypothesis of this study states that the form of the propositions presented in the experiment is critical for calibration phenomena. Affi rmative and negative propositions are...

  5. Calibration Procedure for 3D Turning Dynamometer

    DEFF Research Database (Denmark)

    Axinte, Dragos Aurelian; Belluci, Walter

    1999-01-01

    The aim of the static calibration of the dynamometer is to obtain the matrix for evaluating cutting forces through the output voltage of the piezoelectric cells and charge amplifiers. In the same time, it is worth to evaluate the linearity of the dependencies between applied forces and output...... of the piezoelectric cells;5. Mounting of the dynamometer;6. Calibration of the dynamometer;7. Data analysis;8. Uncertainty budget of the calibration....

  6. Calibration of Nacelle-based Lidar instrument

    DEFF Research Database (Denmark)

    Yordanova, Ginka; Courtney, Michael

    This report presents the result of the lidar calibration performed for a two-beam nacelle based lidar at DTU’s test site for large wind turbines at Høvsøre, Denmark. Calibration is here understood as the establishment of a relation between the reference wind speed measurements with measurement...... uncertainties provided by measurement standard and corresponding lidar wind speed indications with associated measurement uncertainties. The lidar calibration concerns the 10 minute mean wind speed measurements....

  7. 1987 calibration of the TFTR neutron spectrometers

    Energy Technology Data Exchange (ETDEWEB)

    Barnes, C.W.; Strachan, J.D. (Los Alamos National Lab., NM (USA); Princeton Univ., NJ (USA). Plasma Physics Lab.)

    1989-12-01

    The {sup 3}He neutron spectrometer used for measuring ion temperatures and the NE213 proton recoil spectrometer used for triton burnup measurements were absolutely calibrated with DT and DD neutron generators placed inside the TFTR vacuum vessel. The details of the detector response and calibration are presented. Comparisons are made to the neutron source strengths measured from other calibrated systems. 23 refs., 19 figs., 6 tabs.

  8. Radiocarbon calibration - past, present and future

    Energy Technology Data Exchange (ETDEWEB)

    Plicht, J. van der E-mail: plicht@phys.rug.nl

    2004-08-01

    Calibration of the Radiocarbon timescale is traditionally based on tree-rings dated by dendrochronology. At present, the tree-ring curve dates back to about 9900 BC. Beyond this limit, marine datasets extend the present calibration curve INTCAL98 to about 15 600 years ago. Since 1998, a wealth of AMS measurements became available, covering the complete {sup 14}C dating range. No calibration curve can presently be recommended for the older part of the dating range until discrepancies are resolved.

  9. Variability of assay methods for total and free PSA after WHO standardization.

    Science.gov (United States)

    Foj, L; Filella, X; Alcover, J; Augé, J M; Escudero, J M; Molina, R

    2014-03-01

    The variability of total PSA (tPSA) and free PSA (fPSA) results among commercial assays has been suggested to be decreased by calibration to World Health Organization (WHO) reference materials. To characterize the current situation, it is necessary to know its impact in the critical cutoffs used in clinical practice. In the present study, we tested 167 samples with tPSA concentrations of 0 to 20 μg/L using seven PSA and six fPSA commercial assays, including Access, ARCHITECT i2000, ADVIA Centaur XP, IMMULITE 2000, Elecsys, and Lumipulse G1200, in which we only measured tPSA. tPSA and fPSA were measured in Access using the Hybritech and WHO calibrators. Passing-Bablok analysis was performed for PSA, and percentage of fPSA with the Hybritech-calibrated access comparison assay. For tPSA, relative differences were more than 10 % at 0.2 μg/L for ARCHITECT i2000, and at a critical concentration of 3, 4, and 10 μg/L, the relative difference was exceeded by ADVIA Centaur XP and WHO-calibrated Access. For percent fPSA, at a critical concentration of 10 %, the 10 % relative difference limit was exceeded by IMMULITE 2000 assay. At a critical concentration of 20 and 25 %, ADVIA Centaur XP, ARCHITECT i2000, and IMMULITE 2000 assays exceeded the 10 % relative difference limit. We have shown significant discordances between assays included in this study despite advances in standardization conducted in the last years. Further harmonization efforts are required in order to obtain a complete clinical concordance.

  10. Diversity in Organizations

    OpenAIRE

    Antonio S. Mello; Ruckes, Martin

    2001-01-01

    This Paper develops a theory of diversity in work groups within organizations. Diversity is determined by the group members' dfferences in backgrounds. Diverse teams possess more information than homogeneous ones. If beliefs and preferences are expressed openly, diverse teams can reach better decisions. However, due to their members' heterogeneous backgrounds diverse teams are more prone to conflict. The Paper shows that the relative performance of heterogeneous and homogeneous groups depends...

  11. MULTIPLE ELECTRONIC CONTROL UNITS CALIBRATION SYSTEM BASED ON EXPLICIT CALIBRATION PROTOCOL AND J1939 PROTOCOL

    Institute of Scientific and Technical Information of China (English)

    YANG Shiwei; ZHU Keqing; XU Quankui; YANG Lin; ZHUO Bin

    2008-01-01

    The rising number of electronic control units (ECUs) in vehicles and the decreasing time to market have led to the need for advanced methods of calibration. A multi-ECU calibration system was developed based on the explicit calibration protocol (XCP) and J1939 communication protocol to satisfy the need of calibrating multiple ECUs simultaneously. The messages in the controller area network (CAN) are defined in the J1939 protocol. Each CAN node can get its own calibration messages and information from other ECUs, and block other messages by qualifying the CAN messages with priority, source or destination address. The data field of the calibration message is designed with the XCP , with CAN acting as the transport layer. The calibration sessions are setup with the event-triggered XCP driver in the master node and the responding XCP driver in the slave nodes. Mirroring calibration variables from ROM to RAM enables the user to calibrate ECUs online. The application example shows that the multi-ECU calibration system can calibrate multiple ECUs simultaneously, and the main program can also accomplish its calculation and send commands to the actuators in time. By the multi-ECU calibration system, the calibration effort and time can be reduced and the variables in ECU can get a better match with the variables of other ECUs.

  12. Efficiency calibration of low background gamma spectrometer

    International Nuclear Information System (INIS)

    A method of efficiency calibration is described. The authors used standard ores of U, Ra and Th (power form), KCl and Cs-137 sources to do calibration volume-sources which were directly placed on the detector end cap. In such a measuring geometry, it is not necessary to make coincidence-summing correction. The efficiency calibration curve obtained by the method were compared with results measured by Am-241, Cd-109 and Eu-152 calibration sources. The agree in the error of about 5%

  13. Calibration metrology for fixed irradiation sensors

    International Nuclear Information System (INIS)

    After having recalled the regulatory and technical framework of the calibration of radioprotection measurement instruments, and outlined some technical and operational constraints, the authors report the development of an in situ calibration methodology, i.e. without displacement of the sensor. After the presentation of the calibration chain (from the measurement given by a fixed sensor to the reference value given by a primary standard), they indicate the definition and calculation of the different calibration coefficients allowing the linking up of the different levels, and also the taking of uncertainties into account. They finally report the validation of results

  14. The calibration system for the GERDA experiment

    International Nuclear Information System (INIS)

    The GERDA experiment uses the neutrinoless double beta decay to probe three fundamental questions in neutrino physics - Are they Dirac or Majorana particles? What is their absolute mass? What is the mass hierarchy of the three generations? In my talk I present the calibration system for the Ge semiconductor diodes enriched in Ge-76. The system is used to set the energy scale and calibrate the pulse shapes which will be used to further reject background events. The lowest possible background is crucial for the whole experiment and therefore the calibration system must not interfere with the data acquisition phase while at the same time operate efficiently during the calibration runs.

  15. Research of Camera Calibration Based on DSP

    OpenAIRE

    Zheng Zhang; Yukun Wan; Lixin Cai

    2013-01-01

    To take advantage of the high-efficiency and stability of DSP in the data processing and the functions of OpenCV library, this study brought forward a scheme that camera calibration in DSP embedded system calibration. An arithmetic of camera calibration based on OpenCV is designed by analyzing the camera model and lens distortion. The transplantation of EMCV to DSP is completed and the arithmetic of camera calibration is migrated and optimized based on the CCS development environment and the ...

  16. Sloan Digital Sky Survey Photometric Calibration Revisited

    Energy Technology Data Exchange (ETDEWEB)

    Marriner, John; /Fermilab

    2012-06-29

    The Sloan Digital Sky Survey calibration is revisited to obtain the most accurate photometric calibration. A small but significant error is found in the flat-fielding of the Photometric telescope used for calibration. Two SDSS star catalogs are compared and the average difference in magnitude as a function of right ascension and declination exhibits small systematic errors in relative calibration. The photometric transformation from the SDSS Photometric Telescope to the 2.5 m telescope is recomputed and compared to synthetic magnitudes computed from measured filter bandpasses.

  17. FY2008 Calibration Systems Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Cannon, Bret D.; Myers, Tanya L.; Broocks, Bryan T.

    2009-01-01

    The Calibrations project has been exploring alternative technologies for calibration of passive sensors in the infrared (IR) spectral region. In particular, we have investigated using quantum cascade lasers (QCLs) because these devices offer several advantages over conventional blackbodies such as reductions in size and weight while providing a spectral source in the IR with high output power. These devices can provide a rapid, multi-level radiance scheme to fit any nonlinear behavior as well as a spectral calibration that includes the fore-optics, which is currently not available for on-board calibration systems.

  18. Absolute calibration of the Auger fluorescence detectors

    Energy Technology Data Exchange (ETDEWEB)

    Bauleo, P.; Brack, J.; Garrard, L.; Harton, J.; Knapik, R.; Meyhandan, R.; Rovero, A.C.; /Buenos Aires, IAFE; Tamashiro, A.; Warner, D.

    2005-07-01

    Absolute calibration of the Pierre Auger Observatory fluorescence detectors uses a light source at the telescope aperture. The technique accounts for the combined effects of all detector components in a single measurement. The calibrated 2.5 m diameter light source fills the aperture, providing uniform illumination to each pixel. The known flux from the light source and the response of the acquisition system give the required calibration for each pixel. In the lab, light source uniformity is studied using CCD images and the intensity is measured relative to NIST-calibrated photodiodes. Overall uncertainties are presently 12%, and are dominated by systematics.

  19. Sloan Digital Sky Survey Photometric Calibration Revisited

    Science.gov (United States)

    Marriner, J.

    2016-05-01

    The Sloan Digital Sky Survey calibration is revisited to obtain the most accurate photometric calibration. A small but significant error is found in the flat-fielding of the Photometric telescope used for calibration. Two SDSS star catalogs are compared and the average difference in magnitude as a function of right ascension and declination exhibits small systematic errors in relative calibration. The photometric transformation from the SDSS Photometric Telescope to the 2.5 m telescope is recomputed and compared to synthetic magnitudes computed from measured filter bandpasses.

  20. Calibration services for medical applications of radiation

    Energy Technology Data Exchange (ETDEWEB)

    DeWerd, L.A.

    1993-12-31

    Calibration services for the medical community applications of radiation involve measuring radiation precisely and having traceability to the National Institute of Standards and Technology (NIST). Radiation therapy applications involve the use of ionization chambers and electrometers for external beams and well-type ionization chamber systems as well as radioactive sources for brachytherapy. Diagnostic x-ray applications involve ionization chamber systems and devices to measure other parameters of the x-ray machine, such as non-invasive kVp meters. Calibration laboratories have been established to provide radiation calibration services while maintaining traceability to NIST. New radiation applications of the medical community spur investigation to provide the future calibration needs.

  1. KINIK, Absorber Rod Calibration Kinetics

    International Nuclear Information System (INIS)

    1 - Description of program or function: KINIK is an inverse kinetic code that solves the inverse form of the point kinetic equations using the Runge-Kutta method. An optimization procedure is involved to control the time step and to reduce the running time. Up to 24 delayed neutron groups of different types (in case of heavy water as moderator or beryllium as reflector) are considered. KINIK is commonly applied to determine reactivity worths and to calibrate absorber rods. Following a rod drop, neutron flux or power is recorded as a function of time and used as input. 2 - Method of solution: The inverse point kinetic equations are numerically solved for each time step using the Runge-Kutta method. The input data resulting from measurements are first approximated by polynomials of maximum degree 10 using a least-squares approach

  2. Clustered Calibration: An Improvement to Radio Interferometric Direction Dependent Self-Calibration

    CERN Document Server

    Kazemi, Sanaz; Zaroubi, Saleem

    2013-01-01

    The new generation of radio synthesis arrays, such as LOFAR and SKA, have been designed to surpass existing arrays in terms of sensitivity, angular resolution and frequency coverage. This evolution has led to the development of advanced calibration techniques that ensure the delivery of accurate results at the lowest possible computational cost. However, the performance of such calibration techniques is still limited by the compact, bright sources in the sky, used as calibrators. It is important to have a bright enough source that is well distinguished from the background noise level in order to achieve satisfactory results in calibration. We present "clustered calibration" as a modification to traditional radio interferometric calibration, in order to accommodate faint sources that are almost below the background noise level into the calibration process. The main idea is to employ the information of the bright sources' measured signals as an aid to calibrate fainter sources that are nearby the bright sources...

  3. Design and Calibration of a Cryogenic Blackbody Calibrator at Centimeter Wavelengths

    CERN Document Server

    Kogut, A J; Fixsen, D J; Limon, M; Mirel, P G A; Levin, S; Seiffert, M; Lubin, P M

    2004-01-01

    We describe the design and calibration of an external cryogenic blackbody calibrator used for the first two flights of the Absolute Radiometer for Cosmology, Astrophysics, and Diffuse Emission (ARCADE) instrument. The calibrator consists of a microwave absorber weakly coupled to a superfluid liquid helium bath. Half-wave corrugations viewed 30 deg off axis reduce the return loss below -35 dB. Ruthenium oxide resistive thermometers embedded within the absorber monitor the temperature across the face of the calibrator. The thermal calibration transfers the calibration of a reference thermometer to the flight thermometers using the flight thermometer readout system. Data taken near the superfluid transition in 8 independent calibrations 4 years apart agree within 0.3 mK, providing an independent verification of the thermometer calibration at temperatures near that of the cosmic microwave background.

  4. The physical principles of XRF calibrations

    International Nuclear Information System (INIS)

    Full text: XRF Control and calibration software has come a long way in recent years. Advances in the multiple regression software sophistication and speed of computers have provided an essential resource to the XRF analyst. Over recent years there has been a trend amongst some analysts to develop XRF calibrations based exclusively on the statistical information given by calibration software. Multiple regression statistics are designed for non-correlated data sets but give unpredictable results if there are significant correlations in the standards used. This is typical for calibrations weighted towards certified reference materials (CRM's) which, being natural materials, contain correlated concentrations. Purely statistical methods in calibration development have applicability only over very short concentration ranges and for materials whose composition varies little. Beyond these ranges, the calibration has the potential to be unstable and has been known to produce significant deviations in analysis of unknown samples. The statistical information generated during XRF calibrations can be a very useful tool when used in conjunction with knowledge of the physics behind the correction factors applied. The matrix coefficients represent physical absorption/enhancement effects within the sample and are not arbitrary numbers used to get a good fit to the calibration line. Inappropriate use of matrix factors and overlap factors can produce low RMS values but erroneous results in unknown samples. This talk will contain examples to demonstrate hazards with different calibration strategies and will include coverage of the following topics: physical effects occurring within the sample as a result of X-ray irradiation; use of multiple regression statistics and what role it plays in the calibration; calibration strategies using synthetic and CRM standards; determining appropriate theoretical and semi-empirical matrix corrections and line overlap factors. Copyright (1999

  5. Does Staff Diversity Imply Openness to Diversity?

    Science.gov (United States)

    Lauring, Jakob; Selmer, Jan

    2013-01-01

    Purpose: Post-secondary educational organizations are currently some of the most diverse settings to be found. However, few educational studies have dealt with staff diversity and hardly any has looked outside the USA. The purpose of this paper is to present a study of members of international university departments in Denmark. The authors set out…

  6. Does staff diversity imply openness to diversity?

    DEFF Research Database (Denmark)

    Lauring, Jakob; Selmer, Jan

    2013-01-01

    Purpose – Post-secondary educational organizations are currently some of the most diverse settings to be found. However, few educational studies have dealt with staff diversity and hardly any has looked outside the USA. The purpose of this paper is to present a study of members of international...

  7. CERN Diversity Newsletter - November 2015

    CERN Document Server

    Kaltenhauser, Kristin; CERN. Geneva. HR Department

    2015-01-01

    Quarterly CERN Diversity Newsletter, informing on recent and ongoing diversity activities, and interesting reads, videos and other links related to diversity. Subscribe here: https://diversity.web.cern.ch/2015/07/subscribe-diversity-newsletter

  8. CERN Diversity Newsletter - September 2016

    CERN Document Server

    Guinot, Genevieve

    2016-01-01

    Quarterly CERN Diversity Newsletter, informing on recent and ongoing diversity activities, and interesting reads, videos and other links related to diversity. Subscribe here: https://diversity.web.cern.ch/2015/07/subscribe-diversity-newsletter

  9. CERN Diversity Newsletter - March 2016

    CERN Document Server

    Kaltenhauser, Kristin; CERN. Geneva. HR Department

    2016-01-01

    Quarterly CERN Diversity Newsletter, informing on recent and ongoing diversity activities, and interesting reads, videos and other links related to diversity. Subscribe here: https://diversity.web.cern.ch/2015/07/subscribe-diversity-newsletter

  10. Time-Resolved Fluorescence Assays.

    Science.gov (United States)

    Ma, Chen-Ting; Sergienko, Eduard A

    2016-01-01

    Fluorescence-based detection techniques are popular in high throughput screening due to sensitivity and cost-effectiveness. Four commonly used techniques exist, each with distinct characteristics. Fluorescence intensity assays are the simplest to run, but suffer the most from signal interference. Fluorescence polarization assays show less interference from the compounds or the instrument, but require a design that results in change of fluorophore-containing moiety size and usually have narrow assay signal window. Fluorescence resonance energy transfer (FRET) is commonly used for detecting protein-protein interactions and is constrained not by the sizes of binding partners, but rather by the distance between fluorophores. Time-resolved fluorescence resonance energy transfer (TR-FRET), an advanced modification of FRET approach utilizes special fluorophores with long-lived fluorescence and earns its place near the top of fluorescent techniques list by its performance and robustness, characterized by larger assay window and minimized compound spectral interference. TR-FRET technology can be applied in biochemical or cell-based in vitro assays with ease. It is commonly used to detect modulation of protein-protein interactions and in detection of products of biochemical reactions and cellular activities. PMID:27316992

  11. Commutability of the CRM 470 C-reactive protein value in the Dade Behring N High Sensitivity CRP assay.

    Science.gov (United States)

    Johnson, A Myron; Ledue, Thomas B; Collins, Marilyn F

    2003-02-01

    Certified Reference Material 470 (CRM 470) demonstrates commutability with both the manufacturer's calibrator and with dilutions of serum pools in the Dade Behring N High Sensitivity assay for C-reactive protein (CRP). Both regression and back calibration show similar nonlinearity for all materials, largely due to the method of calibration curve fitting used in this assay. Significant differences in values among the currently available commercial assays can be largely overcome by using appropriate calibration curve fitting and the recommended value transfer protocol, which includes a minimum of two assay runs on each of at least 3 separate days, with weight correction of all reconstitutions and dilutions. An initial weight-corrected dilution should be made each day because of the relatively high level of CRP in CRM 470. In our opinion, the degree of nonlinearity, imprecision, and differences in values in currently available assays renders the use of fixed clinical decision cut-points questionable for high-sensitivity CRP. An alternative approach is suggested.

  12. AXAF calibration: the HXDS flow proportional counters

    Science.gov (United States)

    Wargelin, Bradford J.; Kellogg, Edwin M.; McDermott, Walter C.; Evans, Ian N.; Vitek, S. A.

    1997-07-01

    The design, performance, and calibration of the seven flow proportional counters (FPCs) used during AXAF ground calibration are described. Five of the FPCs served as beam normalization detectors (BNDs), and two were used in the telescope focal plane in combination with a set of apertures to measure the point response functions and effective areas of the AXAF mirrors and transmission gratings. The BNDs also provide standards for determining the effective areas of the several telescope/grating/flight-detector combinations. With useful energy resolution and quantum efficiency over the entire 100-eV to 10 keV AXAF energy band, the FPCs provided most of the data acquired during AXAF calibration. Although the principles of proportional counter operation are relatively simple, AXAF's stringent calibration goals require detailed calibration and modeling of such effects as window- support-wire obscuration, window deformation between the support wires, electron diffusion and avalanche processes, gain nonuniformities, and gas pressure and temperature variations. Detector aperture areas and signal processing deadtime must also be precisely determined, and detector degradation during the many months of AXAF calibration must be prevented. The FPC calibration program is based on measurement of individual components (such as window transmission and aperture size) and the relative quantum efficiencies of complete detector systems, as well as absolute QE calibration of selected detectors at the BESSY synchrotron, an x-ray source of precisely known intensity.

  13. Planck 2013 results. V. LFI calibration

    DEFF Research Database (Denmark)

    Planck Collaboration,; Aghanim, N.; Armitage-Caplan, C.;

    2014-01-01

    We discuss the methods employed to photometrically calibrate the data acquired by the Low Frequency Instrument on Planck. Our calibration is based on the Solar Dipole, caused by motion of the Solar System with respect to the CMB rest frame, which provides a signal of a few mK with the same spectr...

  14. Sky camera geometric calibration using solar observations

    Science.gov (United States)

    Urquhart, Bryan; Kurtz, Ben; Kleissl, Jan

    2016-09-01

    A camera model and associated automated calibration procedure for stationary daytime sky imaging cameras is presented. The specific modeling and calibration needs are motivated by remotely deployed cameras used to forecast solar power production where cameras point skyward and use 180° fisheye lenses. Sun position in the sky and on the image plane provides a simple and automated approach to calibration; special equipment or calibration patterns are not required. Sun position in the sky is modeled using a solar position algorithm (requiring latitude, longitude, altitude and time as inputs). Sun position on the image plane is detected using a simple image processing algorithm. The performance evaluation focuses on the calibration of a camera employing a fisheye lens with an equisolid angle projection, but the camera model is general enough to treat most fixed focal length, central, dioptric camera systems with a photo objective lens. Calibration errors scale with the noise level of the sun position measurement in the image plane, but the calibration is robust across a large range of noise in the sun position. Calibration performance on clear days ranged from 0.94 to 1.24 pixels root mean square error.

  15. Optimal, Reliability-Based Code Calibration

    DEFF Research Database (Denmark)

    Sørensen, John Dalsgaard

    2002-01-01

    Reliability based code calibration is considered in this paper. It is described how the results of FORM based reliability analysis may be related to the partial safety factors and characteristic values. The code calibration problem is presented in a decision theoretical form and it is discussed how...

  16. Lidar to lidar calibration phase 2

    DEFF Research Database (Denmark)

    Yordanova, Ginka; Courtney, Michael

    This report presents the results from phase 2 of a lidar to lidar (L2L) calibration procedure. Phase two of the project included two measurement campaigns conducted at given sites. The purpose was to find out if the lidar-to-lidar calibration procedure can be conducted with similar results...

  17. The long term stability of lidar calibrations

    DEFF Research Database (Denmark)

    Courtney, Michael; Gayle Nygaard, Nicolai

    Wind lidars are now used extensively for wind resource measurements. One of the requirements for the data to be accepted in support of project financing (so-called ‘banka-bility’) is to demonstrate the long-term stability of lidar cali-brations. Calibration results for six Leosphere WindCube li...

  18. Microfabricated field calibration assembly for analytical instruments

    Science.gov (United States)

    Robinson, Alex L.; Manginell, Ronald P.; Moorman, Matthew W.; Rodacy, Philip J.; Simonson, Robert J.

    2011-03-29

    A microfabricated field calibration assembly for use in calibrating analytical instruments and sensor systems. The assembly comprises a circuit board comprising one or more resistively heatable microbridge elements, an interface device that enables addressable heating of the microbridge elements, and, in some embodiments, a means for positioning the circuit board within an inlet structure of an analytical instrument or sensor system.

  19. 14 CFR 33.45 - Calibration tests.

    Science.gov (United States)

    2010-01-01

    ... Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: AIRCRAFT ENGINES Block Tests; Reciprocating Aircraft Engines § 33.45 Calibration tests. (a) Each engine must be subjected to the calibration tests necessary to establish its power characteristics...

  20. Calibration of Ground-based Lidar instrument

    DEFF Research Database (Denmark)

    Yordanova, Ginka; Gómez Arranz, Paula

    This report presents the result of the lidar calibration performed for the given Ground-based Lidar at DTU’s test site for large wind turbines at Høvsøre, Denmark. Calibration is here understood as the establishment of a relation between the reference wind speed measurements with measurement unce...

  1. Calibration of Nacelle-based Lidar instrument

    DEFF Research Database (Denmark)

    Yordanova, Ginka; Courtney, Michael

    This report presents the result of the lidar calibration performed for a two-beam nacelle based lidar at DTU’s test site for large wind turbines at Høvsøre, Denmark. Calibration is here understood as the establishment of a relation between the reference wind speed measurements with measurement un...

  2. Radiocarbon calibration - past, present and future

    NARCIS (Netherlands)

    van der Plicht, J

    2004-01-01

    Calibration of the Radiocarbon timescale is traditionally based on tree-rings dated by dendrochronology. At present, the tree-ring curve dates back to about 9900 BC. Beyond this limit, marine datasets extend the present calibration curve INTCAL98 to about 15600 years ago. Since 1998, a wealth of AMS

  3. Building X-ray Diffraction Calibration Software

    Energy Technology Data Exchange (ETDEWEB)

    Lande, Joshua; /Marlboro Coll.

    2007-10-31

    X-ray diffraction is a technique used to analyze the structure of crystals. It records the interference pattern created when x-rays travel through a crystal. Three dimensional structure can be inferred from these two dimensional diffraction patterns. Before the patterns can be analyzed, diffraction data must be precisely calibrated. Calibration is used to determine the experimental parameters of the particular experiment. This is done by fitting the experimental parameters to the diffraction pattern of a well understood crystal. Fit2D is a software package commonly used to do this calibration but it leaves much to be desired. In particular, it does not give very much control over the calibration of the data, requires a significant amount of manual input, does not allow for the calibration of highly tilted geometries, does not properly explain the assumptions that it is making, and cannot be modified. We build code to do this calibration while at the same time overcoming the limitations of Fit2D. This paper describes the development of the calibration software and the assumptions that are made in doing the calibration.

  4. Astronomical calibration of the Maastrichtian (Late Cretaceous)

    DEFF Research Database (Denmark)

    Husson, Dorothée; Galbrun, Bruno; Laskar, Jacques;

    2011-01-01

    Recent improvements to astronomical modeling of the Solar System have contributed to important refinements of the Cenozoic time scale through astronomical calibration of sedimentary series. We extend this astronomical calibration into the Cretaceous, on the base of the 405 ka orbital eccentricity...

  5. The calibration of portable and airborne gamma-ray spectrometers - theory, problems, and facilities

    International Nuclear Information System (INIS)

    A gamma-ray spectrometer for use in geological exploration possesses four stripping ratios and three window sensitivities which must be determined to make the instrumentation applicable for field assay or airborne measurement of potassium, uranium, and thorium contents in the ground. Survey organizations in many parts of the world perform the instrument calibration using large pads of concrete which simulate a plane ground of known radioelement concentration. Calibration and monitoring trials with twelve facilities in ten countries prove that moisture absorption, radon exhalation, and particle-size effects can offset a radiometric grade assigned to concrete whose aggregate contains an embedded radioactive mineral. These and other calibration problems are discussed from a combined theoretical and practical viewpoint. (author)

  6. Design and use of concrete pads for the calibration of radiometric survey instrumentation

    International Nuclear Information System (INIS)

    A gamma-ray spectrometer for use in geological exploration possesses four stripping ratios and three window sensitivities which must be determined to make the instrumentation applicable for field assay or airborne measurement of potassium, uranium, and thorium contents in the ground. Survey organizations in many parts of the world perform the instrument calibration using large pads of concrete which simulate a plane ground of known radioelement concentration. Calibration and monitoring trials with twelve facilities in ten countries prove that moisture absorption, radon exhalation, and particle-size effects can offset a radiometric grade assigned to concrete whose aggregate contains an embedded radioactive mineral. These and other calibration problems are discussed from a combined theoretical and practical viewpoint

  7. Barcoded microchips for biomolecular assays.

    Science.gov (United States)

    Zhang, Yi; Sun, Jiashu; Zou, Yu; Chen, Wenwen; Zhang, Wei; Xi, Jianzhong Jeff; Jiang, Xingyu

    2015-01-20

    Multiplexed assay of analytes is of great importance for clinical diagnostics and other analytical applications. Barcode-based bioassays with the ability to encode and decode may realize this goal in a straightforward and consistent manner. We present here a microfluidic barcoded chip containing several sets of microchannels with different widths, imitating the commonly used barcode. A single barcoded microchip can carry out tens of individual protein/nucleic acid assays (encode) and immediately yield all assay results by a portable barcode reader or a smartphone (decode). The applicability of a barcoded microchip is demonstrated by human immunodeficiency virus (HIV) immunoassays for simultaneous detection of three targets (anti-gp41 antibody, anti-gp120 antibody, and anti-gp36 antibody) from six human serum samples. We can also determine seven pathogen-specific oligonucleotides by a single chip containing both positive and negative controls.

  8. DECal: A Spectrophotometric Calibration System For DECam

    CERN Document Server

    Marshall, J L; DePoy, D L; Prochaska, Travis; Allen, Richard; Behm, Tyler W; Martin, Emily C; Veal, Brannon; Villanueva,, Steven; Williams, Patrick; Wise, Jason

    2013-01-01

    DECal is a new calibration system for the CTIO 4 m Blanco telescope. It is currently being installed as part of the Dark Energy Survey and will provide both broadband flat fields and narrowband (about 1 nm bandwidth) spectrophotometric calibration for the new Dark Energy Camera (DECam). Both of these systems share a new Lambertian flat field screen. The broadband flat field system uses LEDs to illuminate each photometric filter. The spectrophotometric calibration system consists of a monochromator-based tunable light source that is projected onto the flat field screen using a custom line-to-spot fiber bundle and an engineered diffuser. Several calibrated photodiodes positioned along the beam monitor the telescope throughput as a function of wavelength. This system will measure the wavelength-dependent instrumental response function of the total telescope+instrument system in the range 300 < lambda < 1100nm. The spectrophotometric calibration will be performed regularly (roughly once per month) to determ...

  9. Systems and methods of eye tracking calibration

    DEFF Research Database (Denmark)

    2014-01-01

    Methods and systems to facilitate eye tracking control calibration are provided. One or more objects are displayed on a display of a device, where the one or more objects are associated with a function unrelated to a calculation of one or more calibration parameters. The one or more calibration...... parameters relate to a calibration of a calculation of gaze information of a user of the device, where the gaze information indicates where the user is looking. While the one or more objects are displayed, eye movement information associated with the user is determined, which indicates eye movement of one...... or more eye features associated with at least one eye of the user. The eye movement information is associated with a first object location of the one or more objects. The one or more calibration parameters are calculated based on the first object location being associated with the eye movement information....

  10. Parallel Calibration for Sensor Array Radio Interferometers

    CERN Document Server

    Brossard, Martin; Pesavento, Marius; Boyer, Rémy; Larzabal, Pascal; Wijnholds, Stefan J

    2016-01-01

    In order to meet the theoretically achievable imaging performance, calibration of modern radio interferometers is a mandatory challenge, especially at low frequencies. In this perspective, we propose a novel parallel iterative multi-wavelength calibration algorithm. The proposed algorithm estimates the apparent directions of the calibration sources, the directional and undirectional complex gains of the array elements and their noise powers, with a reasonable computational complexity. Furthermore, the algorithm takes into account the specific variation of the aforementioned parameter values across wavelength. Realistic numerical simulations reveal that the proposed scheme outperforms the mono-wavelength calibration scheme and approaches the derived constrained Cram\\'er-Rao bound even with the presence of non-calibration sources at unknown directions, in a computationally efficient manner.

  11. Dutch X-band SLAR calibration

    Science.gov (United States)

    Groot, J. S.

    1990-01-01

    In August 1989 the NASA/JPL airborne P/L/C-band DC-8 SAR participated in several remote sensing campaigns in Europe. Amongst other test sites, data were obtained of the Flevopolder test site in the Netherlands on August the 16th. The Dutch X-band SLAR was flown on the same date and imaged parts of the same area as the SAR. To calibrate the two imaging radars a set of 33 calibration devices was deployed. 16 trihedrals were used to calibrate a part of the SLAR data. This short paper outlines the X-band SLAR characteristics, the experimental set-up and the calibration method used to calibrate the SLAR data. Finally some preliminary results are given.

  12. Electromagnetic Calorimeter Calibration with $\\pi^{0}$

    CERN Multimedia

    Puig Navarro, A

    2009-01-01

    Several methods can be used in order to achieve precise calibration of the LHCb Electromagnetic Calorimeter (ECAL) once reasonable cell equalization has been reached. At low transverse energy, the standard calibration procedure is an iterative method based on the fit of the $\\gamma\\gamma$ invariant mass distribution for each cell of the decay $\\pi^{0}\\to\\gamma\\gamma$ with resolved photons. A new technique for generating the combinatorial background of such decays directly from data has been developed. Knowledge of the background could allow an alternative calibration method based on a event by event fit of the same $\\gamma\\gamma$ invariant mass distribution where contributions from groups of cells are considered in a single fit. The background generation procedure and this possible new calibration method are presented in this poster, in addition to an overview of the LHCb Calorimetry system and ECAL calibration techniques.

  13. The ATLAS Electromagnetic Calorimeter Calibration Workshop

    CERN Multimedia

    Hong Ma; Isabelle Wingerter

    The ATLAS Electromagnetic Calorimeter Calibration Workshop took place at LAPP-Annecy from the 1st to the 3rd of October; 45 people attended the workshop. A detailed program was setup before the workshop. The agenda was organised around very focused presentations where questions were raised to allow arguments to be exchanged and answers to be proposed. The main topics were: Electronics calibration Handling of problematic channels Cluster level corrections for electrons and photons Absolute energy scale Streams for calibration samples Calibration constants processing Learning from commissioning Forty-five people attended the workshop. The workshop was on the whole lively and fruitful. Based on years of experience with test beam analysis and Monte Carlo simulation, and the recent operation of the detector in the commissioning, the methods to calibrate the electromagnetic calorimeter are well known. Some of the procedures are being exercised in the commisssioning, which have demonstrated the c...

  14. Calibration biases in logical reasoning tasks

    Directory of Open Access Journals (Sweden)

    Guillermo Macbeth

    2013-08-01

    Full Text Available The aim of this contribution is to present an experimental study about calibration in deductive reasoning tasks. Calibration is defi ned as the empirical convergence or divergence between the objective and the subjective success. The underconfi dence bias is understood as the dominance of the former over the latter. The hypothesis of this study states that the form of the propositions presented in the experiment is critical for calibration phenomena. Affi rmative and negative propositions are distinguished in their cognitive processing. Results suggests that monotonous compound propositions are prone to underconfi dence. An heuristic approach to this phenomenon is proposed. The activation of a monotony heuristic would produce an illusion of simplicity that generates the calibration bias. These evidence is analysed in the context of the metacognitive modeling of calibration phenomena.

  15. Assessing students' metacognitive calibration with knowledge surveys

    Science.gov (United States)

    Lindsey, Beth A.; Nagel, Megan

    2013-01-01

    "Calibration" is an aspect of metacognition that describes how well students assess their own knowledge. One tool that can help to assess student calibration is the knowledge survey (KS). On a KS, students rate their confidence in their ability to answer questions related to course content. A comparison of a student's confidence level with their actual performance on course exams gives an indication of the student's metacognitive calibration. We report on a study that explores students' responses to a KS in introductory physics and chemistry courses serving both STEM and non-STEM populations. In many courses, Delta (the difference between KS-score and final exam score, a measure of calibration) was anti-correlated with final exam performance. No relationship was found between Delta and students' scientific reasoning abilities. We also report preliminary findings on how calibration differs for questions of a quantitative nature vs. those of a more conceptual nature.

  16. Calibration of phase detector using IQ modulator

    International Nuclear Information System (INIS)

    The beam energy of the J-PARC Linac is calculated by TOF (Time-of-Flight) method with the flight distance and beam phases at the two of measurement points. Because the accuracy of the beam energy measurement is directly depending on the errors in the phase measurement system, all 111 beam phase monitors are calibrated annually. Here, we adopted a calibration method using the IQ modulator as a method for carrying out more simply and accurately, calibration of the phase detection circuit is provided to the phase detection system. In the calibration, we have used the trombone circuit for the adjustment of the reference frequency, but it is thought that the procedure using an IQ modulator is more simple and accurate, and it reduces the time for the calibration. We describe the procedure of the phase detection system and the method of energy calculation. In addition, the general descriptions of the IQ modulation specification and its performances are introduced. (author)

  17. Absolute sensitivity calibration of extreme ultraviolet photoresists

    Energy Technology Data Exchange (ETDEWEB)

    Jones, Juanita; Naulleau, Patrick P.; Gullikson, Eric M.; Aquila, Andrew; George, Simi; Niakoula, Dimitra

    2008-05-16

    One of the major challenges facing the commercialization of extreme ultraviolet (EUV) lithography remains simultaneously achieving resist sensitivity, line-edge roughness, and resolution requirement. Sensitivity is of particular concern owing to its direct impact on source power requirements. Most current EUV exposure tools have been calibrated against a resist standard with the actual calibration of the standard resist dating back to EUV exposures at Sandia National Laboratories in the mid 1990s. Here they report on an independent sensitivity calibration of two baseline resists from the SEMATECH Berkeley MET tool performed at the Advanced Light Source Calibrations and Standards beamline. The results show the baseline resists to be approximately 1.9 times faster than previously thought based on calibration against the long standing resist standard.

  18. Calibration of Sr-90 ophthalmic applicators

    International Nuclear Information System (INIS)

    The purpose of this paper is to alert users of Sr-90 ophthalmic applicators about potential large errors in calibration of these devices. A discrepancy of more than 50% in calibration of Sr-90 ophthalmic applicators between the US National Institute of Standards and Technology (NIST) and one foreign manufacturer (the world's only remaining supplier) has been reported. A single-plane Sr-90 ophthalmic applicator was calibrated by the manufacturer, by NIST, and by the University of Wisconsin. The manufacturer's close rate calibration is confined to a 3-mm-diameter active area, while NIST measures all beta radiation emitted into a 2-PI solid angle. The discrepancy was verified by means of a technique based on that of NIST. Reports of calibrations at NIST of applicators made by several American manufacturers (no longer available) indicate that large discrepancies exist for other manufacturers as well

  19. Selection of stars to calibrate Gaia

    Science.gov (United States)

    Carrasco, J. M.; Voss, H.; Jordi, C.; Fabricius, C.; Pancino, E.; Altavilla, G.

    2015-05-01

    Gaia is an all-sky survey satellite, launched by ESA on 19th December 2013, to obtain parallaxes and proper motions to microarcsecond level precision, radial velocities and astrophysical parameters for about one billion objects down to a limiting magnitude of 20. The chosen strategy to perform the photometric calibration is to split the process into two steps, internal and external calibration. The internal calibration will combine all different transits of a given source to a common reference internal system producing a 'mean' Gaia observation. This internal calibration accounts for the differential instrumental effects (in sensitivity, aperture, PSF, etc.). They depend on the colour and type of the source. For this reason, a selection of calibration sources ensuring a good representation of all kind of observed sources is needed. The entire magnitude and colour range of the sources have to be covered by these calibration stars and for all calibration intervals. It is a challenge to obtain a suitable colour distribution for the standards, especially for bright sources and the daily large scale calibration intervals. Once the mean Gaia observations are produced, a final step, the external calibration, transforms them to absolute fluxes and wavelengths. In principle, few calibration sources are needed (about 200 spectrophotometric standard stars, SPSS, are currently being considered). They need to have accurate determinations of their absolute fluxes and their non-variability need to be ensured below 1% precision. For this purpose, a big international observational effort is being done (using telescopes as 2.2m@CAHA, TNG@LaPalma, NTT@LaSilla, LaRuca@SPM, and others). During this observational effort some cases of non-expected variability of the SPSS candidates have been discovered.

  20. Development of the Abbott MATRIX Aero assay for the measurement of specific IgE.

    Science.gov (United States)

    Lindberg, R E; Anawis, M A; Bailey, M; Mangat, D; Frank, P M; Hrusovsky, I G; Hooyman, L; Putterman, C; Defreese, J D

    1991-01-01

    An enzyme immunoassay has been developed for the quantitation of specific immunoglobulin E (IgE) in human serum to a panel of allergens. The assay system, called the Abbott MATRIX Aero, includes an instrument, reagents and test cell disposables. Each test cell contains fourteen airborne allergens individually localized on a nitrocellulose solid phase. Individual calibration curves for each allergen are established by the manufacturer and included in barcode form with each test kit. Stable factory calibration eliminates the need to establish a calibration curve with each assay run. The instrument automatically incubates, washes, and reads the test cell and prints each result, which ensures assay reproducibility and provides ease-of-use. Analysis of test results shows good agreement with another in vitro assay for specific IgE. The Abbott MATRIX Aero is a sensitive, reproducible and easy-to-use system for the measurement of specific IgE to a panel of fourteen allergens simultaneously using a single, small volume of serum. PMID:1806584

  1. Automation of dosimeters calibration for radiotherapy in secondary dosimetric calibration laboratory of the CPHR

    International Nuclear Information System (INIS)

    This paper presents the design and implementation of an automated system for measurements in the calibration of reference radiation dosimeters. It was made a software application that performs the acquisition of the measured values of electric charge, calculated calibration coefficient and automates the calibration certificate issuance. These values are stored in a log file on a PC. The use of the application improves control over the calibration process, helps to humanize the work and reduces personnel exposure. The tool developed has been applied to the calibration of dosimeters radiation patterns in the LSCD of the Centro de Proteccion e Higiene de las Radiaciones, Cuba

  2. Managing Workplace Diversity

    Directory of Open Access Journals (Sweden)

    Harold Andrew Patrick

    2012-04-01

    Full Text Available Diversity management is a process intended to create and maintain a positive work environment where the similarities and differences of individuals are valued. The literature on diversity management has mostly emphasized on organization culture; its impact on diversity openness; human resource management practices; institutional environments and organizational contexts to diversity-related pressures, expectations, requirements, and incentives; perceived practices and organizational outcomes related to managing employee diversity; and several other issues. The current study examines the potential barriers to workplace diversity and suggests strategies to enhance workplace diversity and inclusiveness. It is based on a survey of 300 IT employees. The study concludes that successfully managing diversity can lead to more committed, better satisfied, better performing employees and potentially better financial performance for an organization.

  3. Putting Diversity to Work

    DEFF Research Database (Denmark)

    Holck, Lotte

    2016-01-01

    organizational approval of a diversity agenda, open-mindedness towards change and legitimacy in regard to diversity. Paradoxically, change efforts designed to alter the status quo were, in practice, derailed and circumvented through power dynamics reproducing organizational inequality. Research limitations...

  4. A Cryogenic Infrared Calibration Target

    CERN Document Server

    Wollack, Edward J; Rinehart, Stephan A

    2014-01-01

    A compact cryogenic calibration target is presented that has a peak diffuse reflectance, $R \\le 0.003$, from $800-4,800\\,{\\rm cm}^{-1}$ $(12-2\\,\\mu$m). Upon expanding the spectral range under consideration to $400-10,000\\,{\\rm cm}^{-1}$ $(25-1\\,\\mu$m) the observed performance gracefully degrades to $R \\le 0.02$ at the band edges. In the implementation described, a high-thermal-conductivity metallic substrate is textured with a pyramidal tiling and subsequently coated with a thin lossy dielectric coating that enables high absorption and thermal uniformity across the target. The resulting target assembly is lightweight, has a low-geometric profile, and has survived repeated thermal cycling from room temperature to $\\sim4\\,$K. Basic design considerations, governing equations, and test data for realizing the structure described are provided. The optical properties of selected absorptive materials -- Acktar Fractal Black, Aeroglaze Z306, and Stycast 2850 FT epoxy loaded with stainless steel powder -- are character...

  5. HIBP calibration on WEGA stellarator

    Energy Technology Data Exchange (ETDEWEB)

    Podoba, Yuriy; Otte, Matthias; Wagner, Friedrich [Max-Planck-Institut fuer Plasmaphysik, Greifswald (Germany); Zhezhera, Alexander; Chmyga, Alexander; Kozachok, Alexander; Komarov, Alexander; Bondarenko, Ivan; Deshko, Galina; Khrebtov, Sergey; Krupnik, Ludmila [Kharkov Institute of Plasma Physics, Kharkov (Ukraine)

    2009-07-01

    The heavy ion beam probe (HIBP) is an established non-perturbing diagnostic for determining spatial distributions of plasma potential, density, temperature and poloidal magnetic field of magnetically confined plasmas. These quantities can be determined from the change in the ion beam parameters (charge, intensity and trajectory) passing through a plasma volume due to collisions with electrons and interactions with the confining magnetic field. The WEGA HIBP operates with a Na{sup +} with an energy of 39.5 keV and beam current 35 {mu}A. Conventionally the coordinate mapping of the HIBP is provided by ray tracing calculations of the ion beam in the magnetic field. However, it is very difficult to include all physical effects in the model, thus the result of the calculations may significantly differ from the real probing position. In order to improve the mapping precision an additional measurements of the beam position have been provided using a beam detector array inside the vacuum vessel. This allows to compare the measured and calculated ion beam position in order to find out the reasons for the coordinate mismatch and include adjustments in the calculation code. Results of this calibration are presented in this work.

  6. SMAP Radar Processing and Calibration

    Science.gov (United States)

    West, R.; Jaruwatanadilok, S.; Kwoun, O.; Chaubell, M.

    2013-01-01

    The Soil Moisture Active Passive (SMAP) mission is part of the NASA space-based Earth observation program, and consists of an L-band radar and radiometer scheduled for launch into sun synchronous orbit in late 2014. A joint effort of the Jet Propulsion Laboratory (JPL) and the Goddard Space Flight Center (GSFC), the SMAP mission draws heavily on the design and risk reduction heritage of the Hydrosphere State (Hydros) mission [1], [2]. The SMAP science and applications objectives are to: 1) understand processes that link the terrestrial water, energy and carbon cycles, 2) estimate global water and energy fluxes at the land surface, 3) quantify net carbon flux in boreal landscapes, 4) enhance weather and climate forecast skill, and 5) develop improved flood prediction and drought monitoring capability. To meet these science objectives, SMAP ground processing will combine the attributes of the radar and radiometer observations (in terms of their spatial resolution and sensitivity to soil moisture, surface roughness, and vegetation) to estimate soil moisture with 4% volumetric accuracy at a resolution of 10 km, and freeze-thaw state at a resolution of 1-3 km. Model sensitivities translate the soil moisture accuracy to a radar backscatter accuracy of 1 dB (1 sigma) at 3 km resolution and a brightness temperature accuracy of 1.3 K at 40 km resolution. This paper will describe the level 1 radar processing and calibration challenges and the choices made so far for the algorithms and software implementation.

  7. International diversity management

    DEFF Research Database (Denmark)

    Lauring, Jakob

    2013-01-01

    challenges in balancing between global integration and local responsiveness. The aim of this paper is to illustrate some of the central problems that multinational corporations need to deal with when transferring diversity management practices from headquarters to local subsidiaries. This is illustrated......While the concern with demographic diversity in organizations has increased during recent years, international diversity management still remains an understudied area. This is unfortunate since the transfer of diversity management practices within multinational corporations faces particular...

  8. Diversity does not travel!

    DEFF Research Database (Denmark)

    Lund, Rebecca; Meriläinen, Susan; Tienari, Janne

    2014-01-01

    In this chapter we offer insights into the social construction of diversity in Finnish organizations and society. In Finnish organizations, gender is highlighted while other markers of diversity are blotted out. 'Non-Finns' become subject to cultural assimilation. The US-based concept of Diversity...... Management becomes adopted and adapted in particular ways. Standardized concepts of diversity and its management do not travel, rather they become translated locally. In organizational practice, globalization is slow and laborious....

  9. SPRT Calibration Uncertainties and Internal Quality Control at a Commercial SPRT Calibration Facility

    Science.gov (United States)

    Wiandt, T. J.

    2008-06-01

    The Hart Scientific Division of the Fluke Corporation operates two accredited standard platinum resistance thermometer (SPRT) calibration facilities, one at the Hart Scientific factory in Utah, USA, and the other at a service facility in Norwich, UK. The US facility is accredited through National Voluntary Laboratory Accreditation Program (NVLAP), and the UK facility is accredited through UKAS. Both provide SPRT calibrations using similar equipment and procedures, and at similar levels of uncertainty. These uncertainties are among the lowest available commercially. To achieve and maintain low uncertainties, it is required that the calibration procedures be thorough and optimized. However, to minimize customer downtime, it is also important that the instruments be calibrated in a timely manner and returned to the customer. Consequently, subjecting the instrument to repeated calibrations or extensive repeated measurements is not a viable approach. Additionally, these laboratories provide SPRT calibration services involving a wide variety of SPRT designs. These designs behave differently, yet predictably, when subjected to calibration measurements. To this end, an evaluation strategy involving both statistical process control and internal consistency measures is utilized to provide confidence in both the instrument calibration and the calibration process. This article describes the calibration facilities, procedure, uncertainty analysis, and internal quality assurance measures employed in the calibration of SPRTs. Data will be reviewed and generalities will be presented. Finally, challenges and considerations for future improvements will be discussed.

  10. Beyond fossil calibrations: Realities of molecular clock practices in evolutionary biology

    Directory of Open Access Journals (Sweden)

    Christy Anna Hipsley

    2014-05-01

    Full Text Available Molecular-based divergence dating methods, or molecular clocks, are the primary neontological tool for estimating the temporal origins of clades. While the appropriate use of vertebrate fossils as external clock calibrations has stimulated heated discussions in the paleontological community, less attention has been given to the quality and implementation of other calibration types. In lieu of appropriate fossils, many studies rely on alternative sources of age constraints based on geological events, substitution rates and heterochronous sampling, as well as dates secondarily derived from previous analyses. To illustrate the breadth and frequency of calibration types currently employed, we conducted a literature survey of over 600 articles published from 2007 to 2013. Over half of all analyses implemented one or more fossil dates as constraints, followed by geological events and secondary calibrations (15% each. Vertebrate taxa were subjects of nearly half of all studies, while invertebrates and plants together accounted for 43%, followed by viruses, protists and fungi (3% each. Current patterns in calibration practices were disproportionate to the number of discussions on their proper use, particularly regarding plants and secondarily derived dates, which are both relatively neglected. Based on our survey, we provide a comprehensive overview of the latest approaches in clock calibration, and outline strengths and weaknesses associated with each. This critique should serve as a call to action for researchers across multiple communities, particularly those working on clades for which fossil records are poor, to develop their own guidelines regarding selection and implementation of alternative calibration types. This issue is particularly relevant now, as time-calibrated phylogenies are used for more than dating evolutionary origins, but often serve as the backbone of investigations into biogeography, diversity dynamics and rates of phenotypic

  11. Beyond fossil calibrations: realities of molecular clock practices in evolutionary biology.

    Science.gov (United States)

    Hipsley, Christy A; Müller, Johannes

    2014-01-01

    Molecular-based divergence dating methods, or molecular clocks, are the primary neontological tool for estimating the temporal origins of clades. While the appropriate use of vertebrate fossils as external clock calibrations has stimulated heated discussions in the paleontological community, less attention has been given to the quality and implementation of other calibration types. In lieu of appropriate fossils, many studies rely on alternative sources of age constraints based on geological events, substitution rates and heterochronous sampling, as well as dates secondarily derived from previous analyses. To illustrate the breadth and frequency of calibration types currently employed, we conducted a literature survey of over 600 articles published from 2007 to 2013. Over half of all analyses implemented one or more fossil dates as constraints, followed by geological events and secondary calibrations (15% each). Vertebrate taxa were subjects in nearly half of all studies, while invertebrates and plants together accounted for 43%, followed by viruses, protists and fungi (3% each). Current patterns in calibration practices were disproportionate to the number of discussions on their proper use, particularly regarding plants and secondarily derived dates, which are both relatively neglected in methodological evaluations. Based on our survey, we provide a comprehensive overview of the latest approaches in clock calibration, and outline strengths and weaknesses associated with each. This critique should serve as a call to action for researchers across multiple communities, particularly those working on clades for which fossil records are poor, to develop their own guidelines regarding selection and implementation of alternative calibration types. This issue is particularly relevant now, as time-calibrated phylogenies are used for more than dating evolutionary origins, but often serve as the backbone of investigations into biogeography, diversity dynamics and rates of

  12. Intercomparison and calibration of dose calibrators used in nuclear medicine facilities

    CERN Document Server

    Costa, A M D

    2003-01-01

    The aim of this work was to establish a working standard for intercomparison and calibration of dose calibrators used in most of nuclear medicine facilities for the determination of the activity of radionuclides administered to patients in specific examinations or therapeutic procedures. A commercial dose calibrator, a set of standard radioactive sources, and syringes, vials and ampoules with radionuclide solutions used in nuclear medicine were utilized in this work. The commercial dose calibrator was calibrated for radionuclide solutions used in nuclear medicine. Simple instrument tests, such as linearity response and variation response with the source volume at a constant source activity concentration were performed. This instrument may be used as a reference system for intercomparison and calibration of other activity meters, as a method of quality control of dose calibrators utilized in nuclear medicine facilities.

  13. Intersectionality, Diversity and Gender

    DEFF Research Database (Denmark)

    Siim, Birte; Agustin, Lise Rolandsen

    2016-01-01

    In the discourses of Danish politicians on ethno-national diversity and integration, the notion of diversity is gendered, especially the articulation of the ‘working woman’ and her labor market participation. Equality, diversity and gender are, thus, intertwined in political, discursive construct...

  14. Unity in Diversity.

    Science.gov (United States)

    Purves, Alan C.

    The cultural diversities of peoples and dialects in the United States have brought a richness to the English language that has made it one of the most supple of all the languages in the world. In addition to the diversity in the language are the diversities in literature, technology, nationality, politics, and styles of teaching. Teachers of…

  15. Multilevel and Diverse Classrooms

    Science.gov (United States)

    Baurain, Bradley, Ed.; Ha, Phan Le, Ed.

    2010-01-01

    The benefits and advantages of classroom practices incorporating unity-in-diversity and diversity-in-unity are what "Multilevel and Diverse Classrooms" is all about. Multilevel classrooms--also known as mixed-ability or heterogeneous classrooms--are a fact of life in ESOL programs around the world. These classrooms are often not only multilevel…

  16. Chromosome aberration assays in Allium

    Energy Technology Data Exchange (ETDEWEB)

    Grant, W.F.

    1982-01-01

    The common onion (Allium cepa) is an excellent plant for the assay of chromosome aberrations after chemical treatment. Other species of Allium (A. cepa var. proliferum, A. carinatum, A. fistulosum and A. sativum) have also been used but to a much lesser extent. Protocols have been given for using root tips from either bulbs or seeds of Allium cepa to study the cytological end-points, such as chromosome breaks and exchanges, which follow the testing of chemicals in somatic cells. It is considered that both mitotic and meiotic end-points should be used to a greater extent in assaying the cytogenetic effects of a chemical. From a literature survey, 148 chemicals are tabulated that have been assayed in 164 Allium tests for their clastogenic effect. Of the 164 assays which have been carried out, 75 are reported as giving a positive reaction, 49 positive and with a dose response, 1 positive and temperature-related, 9 borderline positive, and 30 negative; 76% of the chemicals gave a definite positive response. It is proposed that the Allium test be included among those tests routinely used for assessing chromosomal damage induced by chemicals.

  17. Bacterial mutagenicity assays: test methods.

    Science.gov (United States)

    Gatehouse, David

    2012-01-01

    The most widely used assays for detecting chemically induced gene mutations are those employing bacteria. The plate incorporation assay using various Salmonella typhimurium LT2 and E. coli WP2 strains is a short-term bacterial reverse mutation assay specifically designed to detect a wide range of chemical substances capable of causing DNA damage leading to gene mutations. The test is used worldwide as an initial screen to determine the mutagenic potential of new chemicals and drugs.The test uses several strains of S. typhimurium which carry different mutations in various genes of the histidine operon, and E. coli which carry the same AT base pair at the critical mutation site within the trpE gene. These mutations act as hot spots for mutagens that cause DNA damage via different mechanisms. When these auxotrophic bacterial strains are grown on a minimal media agar plates containing a trace of the required amino-acid (histidine or tryptophan), only those bacteria that revert to amino-acid independence (His(+) or Tryp(+)) will grow to form visible colonies. The number of spontaneously induced revertant colonies per plate is relatively constant. However, when a mutagen is added to the plate, the number of revertant colonies per plate is increased, usually in a dose-related manner.This chapter provides detailed procedures for performing the test in the presence and absence of a metabolic activation system (S9-mix), including advice on specific assay variations and any technical problems. PMID:22147566

  18. Assays for B lymphocyte function.

    Science.gov (United States)

    Bondada, Subbarao; Robertson, Darrell A

    2003-11-01

    This unit describes the antigenic stimulation of in vitro antibody production by B cells and the subsequent measurement of secreted antibodies. The first basic protocol is a generalized system for inducing in vitro antibody production and can accommodate various types of antigens under study. Secreted antibodies can then be measured with an enzyme-linked immunosorbent assay (ELISA) or other soluble-antibody detection systems. Alternatively, the number of antibody-producing cells can be quantified by plaque-forming cell (PFC) assays presented in this unit: the Cunningham-Szenberg and the Jerne-Nordin techniques. Both methods employ specially prepared slide chambers, described here, in which the antibody-producing B cells are mixed with complement and indicator sheep red blood cells (SRBC), or with trinitrophenol-modified SRBC (TNP-SRBC), with subsequent lysis and counting of plaques. Because IgM antibodies fix complement efficiently, whereas IgG and IgA antibodies do not, unmodified PFC assays measure only IgM antibodies. The assay can be modified, however, to measure all classes of antibodies or to enumerate total immunoglobulin-secreting B cells, as described in alternate protocols. Yet another method of measuring the number of antibody-producing B cells (in a class-specific fashion) is to use the ELISPOT technique described in UNIT 7.14. The resting B cells used in these procedures are prepared as described in the final support protocols for Percoll gradient centrifugation. PMID:18432909

  19. Assessment of assay sensitivity and precision in a malaria antibody ELISA.

    Science.gov (United States)

    Rajasekariah, G Halli R; Kay, Graeme E; Russell, Natrice V; Smithyman, Anthony M

    2003-01-01

    Many types of ELISA-based immunodiagnostic test kits are commercially available in the market for specific indications. These kits provide necessary assay components, reagents, and guidelines to perform the assay under designated optimal conditions. By using these kits, any unknown or test sample can be assessed as negative or positive based on the results of referral calibrator (Ref+ve and Ref-ve) samples. It is essential to provide reliable test kits to end-users with adequate quality control analysis. Therefore, it is necessary to check the kit for any variations in its performance. While developing a malaria antibody ELISA test-kit, we optimized assay conditions with chequer-board analyses and developed an assay protocol. We have taken out kits randomly from the assembly line and had them evaluated by operators who are new to the test-kits. Assays are performed as per the test guidelines provided. Sera, diluted serially, have shown a clear discriminatory signal between a negative vs. positive sample. A COV is determined by evaluating the Ref-ve calibrator in replicate antigen-coated wells from 6 different plates. This COV is used as a tool to determine S/N ratio of test samples. Besides Ref-ve and Ref+ve calibrators, additional field serum samples are tested with the test kit. Several performance indices, such as mean, standard deviation, %CV are calculated, and the inter- and intra-assay variations determined. The assay precision is determined with large and small replicate samples. In addition, assays are performed concurrently in triplicate-, duplicate-, and single-wells, and the results are analyzed for any assay variations. Different plate areas are identified in antigen-coated 96-well plates and tested blind to detect any variations. The S/N ratio is found to be a very effective tool in determining the assay sensitivity. The %CV was within 10-15%. Variations seen in the assays are found to be due to operator errors and not due to kit reagents. These

  20. Forecast of physicochemical properties and chemical composition of gasoline from infrared spectra, using multivariate calibration; Previsao de propriedades fisico-quimicas e composicao quimica da gasolina a partir de espectros infravermelhos, utilizando calibracao multivariada

    Energy Technology Data Exchange (ETDEWEB)

    Cocco, Lilian Cristina; Yamamoto, Carlos Itsuo [Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil). Lab. de Analise de Combustiveis Automotivos (LACAUTets)

    2008-07-01

    This work describes the attainment of mathematical models, applying multivariate calibration in infrared spectrum with ATR, from 128 gasoline samples with diverse chemical compositions, collected in a period of two and a half years. Infrared spectra had been used to assemble the input matrix for the modeling, whereas the standardized assays and gaseous chromatography had supplied the output matrices. Ninety samples were been used for training and 38 for testing. In order to calibrate chemical composition from chromatography, the techniques of mass spectrometry and chemical ionization were used to identify unknown substances and improve the fitting of the mathematical models. Two hundred and ninety substances were detected and identified, from which 100 were unknown. Six PLS/PCR models were attained to predict some properties as specific mass, Reid vapor pressure, T10, T50, T90 and PFE from distillation curve. Another six PLS/PCR models were attained to predict the amount of aromatics, paraffins, isoparaffins, naphthenes, olefins and oxygenates. In general, mathematical models were attained with good training fit, with correlation coefficients higher than 0,975 (T10) and reaching a maximum of 0,998 (naphthenes) and they are able to forecast an average chemical percentage and properties of interest from gasoline, with acceptable prediction errors. (author)

  1. Calibration of higher eigenmodes of cantilevers

    Science.gov (United States)

    Labuda, Aleksander; Kocun, Marta; Lysy, Martin; Walsh, Tim; Meinhold, Jieh; Proksch, Tania; Meinhold, Waiman; Anderson, Caleb; Proksch, Roger

    2016-07-01

    A method is presented for calibrating the higher eigenmodes (resonant modes) of atomic force microscopy cantilevers that can be performed prior to any tip-sample interaction. The method leverages recent efforts in accurately calibrating the first eigenmode by providing the higher-mode stiffness as a ratio to the first mode stiffness. A one-time calibration routine must be performed for every cantilever type to determine a power-law relationship between stiffness and frequency, which is then stored for future use on similar cantilevers. Then, future calibrations only require a measurement of the ratio of resonant frequencies and the stiffness of the first mode. This method is verified through stiffness measurements using three independent approaches: interferometric measurement, AC approach-curve calibration, and finite element analysis simulation. Power-law values for calibrating higher-mode stiffnesses are reported for several cantilever models. Once the higher-mode stiffnesses are known, the amplitude of each mode can also be calibrated from the thermal spectrum by application of the equipartition theorem.

  2. Preliminary evaluation of a Neutron Calibration Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Alvarenga, Talysson S.; Neves, Lucio P.; Perini, Ana P.; Sanches, Matias P.; Mitake, Malvina B.; Caldas, Linda V.E., E-mail: talvarenga@ipen.br, E-mail: lpneves@ipen.br, E-mail: aperini@ipen.br, E-mail: msanches@ipen.br, E-mail: mbmitake@ipen.br, E-mail: lcaldas@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Federico, Claudio A., E-mail: claudiofederico@ieav.cta.br [Instituto de Estudos Avancados (IEAv/DCTA), Sao Jose dos Campos, SP (Brazil). Dept. de Ciencia e Tecnologia Aeroespacial

    2013-07-01

    In the past few years, Brazil and several other countries in Latin America have experimented a great demand for the calibration of neutron detectors, mainly due to the increase in oil prospection and extraction. The only laboratory for calibration of neutron detectors in Brazil is localized at the Institute for Radioprotection and Dosimetry (IRD/CNEN), Rio de Janeiro, which is part of the IAEA SSDL network. This laboratory is the national standard laboratory in Brazil. With the increase in the demand for the calibration of neutron detectors, there is a need for another calibration services. In this context, the Calibration Laboratory of IPEN/CNEN, Sao Paulo, which already offers calibration services of radiation detectors with standard X, gamma, beta and alpha beams, has recently projected a new calibration laboratory for neutron detectors. In this work, the ambient equivalent dose rate (H⁎(10)) was evaluated in several positions inside and around this laboratory, using Monte Carlo simulation (MCNP5 code), in order to verify the adequateness of the shielding. The obtained results showed that the shielding is effective, and that this is a low-cost methodology to improve the safety of the workers and evaluate the total staff workload. (author)

  3. Increased Automation in Stereo Camera Calibration Techniques

    Directory of Open Access Journals (Sweden)

    Brandi House

    2006-08-01

    Full Text Available Robotic vision has become a very popular field in recent years due to the numerous promising applications it may enhance. However, errors within the cameras and in their perception of their environment can cause applications in robotics to fail. To help correct these internal and external imperfections, stereo camera calibrations are performed. There are currently many accurate methods of camera calibration available; however, most or all of them are time consuming and labor intensive. This research seeks to automate the most labor intensive aspects of a popular calibration technique developed by Jean-Yves Bouguet. His process requires manual selection of the extreme corners of a checkerboard pattern. The modified process uses embedded LEDs in the checkerboard pattern to act as active fiducials. Images are captured of the checkerboard with the LEDs on and off in rapid succession. The difference of the two images automatically highlights the location of the four extreme corners, and these corner locations take the place of the manual selections. With this modification to the calibration routine, upwards of eighty mouse clicks are eliminated per stereo calibration. Preliminary test results indicate that accuracy is not substantially affected by the modified procedure. Improved automation to camera calibration procedures may finally penetrate the barriers to the use of calibration in practice.

  4. Evaluation of 1066 ToxCast Chemicals in a human stem cell assay for developmental toxicity (SOT)

    Science.gov (United States)

    To increase the diversity of assays used to assess potential developmental toxicity, the ToxCast chemical library was screened in the Stemina devTOX quickPREDICT assay using human embryonic stem (hES) cells. A model for predicting teratogenicity was based on a training set of 23 ...

  5. Landsat TM and ETM+ thermal band calibration

    Science.gov (United States)

    Barsi, J.A.; Schott, J.R.; Palluconi, F. D.; Helder, D.L.; Hook, S.J.; Markham, B.L.; Chander, G.; O'Donnell, E. M.

    2003-01-01

    Landsat-5 has been imaging the Earth since March 1984, and Landsat-7 was added to the series of Landsat instruments in April 1999. The Landsat Project Science Office and the Landsat-7 Image Assessment System have been monitoring the on-board calibration of Landsat-7 since launch. Additionally, two separate university teams have been evaluating the on-board thermal calibration of Landsat-7 through ground-based measurements since launch. Although not monitored as closely over its lifetime, a new effort is currently being made to validate the calibration of Landsat-5. Two university teams are beginning to collect ground truth under Landsat-5, along with using other vicarious calibration methods to go back into the archive to validate the history of the calibration of Landsat-5. This paper considers the calibration efforts for the thermal band, band 6, of both the Landsat-5 and Landsat-7 instruments. Though stable since launch, Landsat-7 had an initial calibration error of about 3 K, and changes were made to correct for this beginning 1 October 2000 for data processed with the National Landsat Archive Production System (NLAPS) and beginning 20 December 2000 for data processed with the Landsat Product Generation System (LPGS). Recent results from Landsat-5 vicarious calibration efforts show an offset of –0.7 K over the lifetime of the instrument. This suggests that historical calibration efforts may have been detecting errors in processing systems rather than changes in the instrument. A correction to the Landsat-5 processing has not yet been implemented but will be in the near future.

  6. EFFECTIVE DIVERSITY MANAGEMENT INITIATIVES

    Directory of Open Access Journals (Sweden)

    Suat Begec

    2013-01-01

    Full Text Available Diversity characteristics defines into four areas; personality, internal and external characteristics, and organizational characteristics. Today it is hard to find individuals, organizational and management styles all similar to each other. Twenty-first century leaders face diversity challenges in many arenas and it is a fact that leaders have to live with these diversities. Values affects on the management and organization systems. The global values gain importance and remove the sources of diversities. The leaders believe that the values should be mostly protected. This article focuses on effective diversity management initiatives.

  7. Geometric calibration of ERS satellite SAR images

    DEFF Research Database (Denmark)

    Mohr, Johan Jacob; Madsen, Søren Nørvang

    2001-01-01

    Geometric calibration of the European Remote Sensing (ERS) Satellite synthetic aperture radar (SAR) slant range images is important in relation to mapping areas without ground reference points and also in relation to automated processing. The relevant SAR system parameters are discussed...... and calibrated by using the European Space Agency (ESA) transponders at Flevoland. The resulting accuracy of the slant range images corresponds to 10 m horizontally on the ground. The results are verified by using runway intersections and corner reflectors surveyed with differential GPS techniques. Based......, is described to allow other researchers to geometrically calibrate their processing systems...

  8. Calibration of Ground-based Lidar instrument

    DEFF Research Database (Denmark)

    Yordanova, Ginka; Gómez Arranz, Paula

    This report presents the result of the lidar calibration performed for the given Ground-based Lidar at DTU’s test site for large wind turbines at Høvsøre, Denmark. Calibration is here understood as the establishment of a relation between the reference wind speed measurements with measurement...... uncertainties provided by measurement standard and corresponding lidar wind speed indications with associated measurement uncertainties. The lidar calibration concerns the 10 minute mean wind speed measurements. The comparison of the lidar measurements of the wind direction with that from wind vanes...... measurements are given for information only....

  9. Calibration of Ground-based Lidar instrument

    DEFF Research Database (Denmark)

    Yordanova, Ginka; Gómez Arranz, Paula

    This report presents the result of the lidar calibration performed for the given Ground-based Lidar at DTU’s test site for large wind turbines at Høvsøre, Denmark. Calibration is here understood as the establishment of a relation between the reference wind speed measurements with measurement...... uncertainties provided by measurement standard and corresponding lidar wind speed indications with associated measurement uncertainties. The lidar calibration concerns the 10 minute mean wind speed measurements. The comparison of the lidar measurements of the wind direction with that from wind vanes...

  10. Global analysis of the phase calibration operation

    Science.gov (United States)

    Lannes, André

    2005-04-01

    A global approach to phase calibration is presented. The corresponding theoretical framework calls on elementary concepts of algebraic graph theory (spanning tree of maximal weight, cycles) and algebraic number theory (lattice, nearest lattice point). The traditional approach can thereby be better understood. In radio imaging and in optical interferometry, the self-calibration procedures must often be conducted with much care. The analysis presented should then help in finding a better compromise between the coverage of the calibration graph (which must be as complete as possible) and the quality of the solution (which must of course be reliable).

  11. Calibration for 3D Structured Light Measurement

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    A calibration procedure was developed for three-dimensional(3D) binocular structured light measurement systems. In virtue of a specially designed pattern, matching points in stereo images are extracted. And then sufficient 3D space points are obtained through pairs of images with the intrinsic and extrinsic parameters of each camera estimated prior and consequently some lights are calibrated by means of multi point fitting. Finally, a mathematical model is applied to interpolate and approximate all dynamic scanning lights based on geometry. The process of calibration method is successfully used in the binocular 3D measurement system based on structured lights and the 3D reconstruction results are satisfying.

  12. Design of a neutron source for calibration

    International Nuclear Information System (INIS)

    The neutron spectra produced by an isotopic neutron source located at the center of moderating media were calculated using Monte Carlo method in the aim to design a neutron source for calibration purposes. To improve the evaluation of the dosimetric quantities, is recommended to calibrate the radiation protection devices with calibrated neutron sources whose neutron spectra being similar to those met in practice. Here, a 239Pu-Be neutron source was inserted in H2O, D2O and polyethylene cylindrical moderators in order to produce neutron spectra that resembles spectra found in workplaces

  13. Calibration boards for the ATLAS LAr calorimeters

    International Nuclear Information System (INIS)

    In order to calibrate the ATLAS Liquid Argon (LAr) calorimeters to an accuracy better than 1%, over 16 bit dynamic range, chips have been designed in DMILL technology. The design and performance of a 16 bit DAC, a static low offset operational amplifier and a digital chip to control the calibration boards are presented. A 8 channels board using these chips has also been realised and carefully measured as this module will be replicated 16 times to design the final 128 channels calibration board. (authors)

  14. Implementing a technique to improve the accuracy of shuffler assays of waste drums

    Energy Technology Data Exchange (ETDEWEB)

    Rinard, P.M.

    1996-07-01

    The accuracy of shuffler assays for fissile materials is generally limited by the accuracy of the calibration standards, but when the matrix in a large drum has a sufficiently high hydrogen density (as exists in paper, for example) the accuracy in the active mode can be adversely affected by a nonuniform distribution of the fissile material within the matrix. This paper reports on a technique to determine the distribution nondestructively using delayed neutron signals generated by the shuffler itself. In assays employing this technique, correction factors are applied to the result of the conventional assay according to the distribution. Maximum inaccuracies in assays with a drum of paper, for example, are reduced by a factor of two or three.

  15. An ECVAG trial on assessment of oxidative damage to DNA measured by the comet assay

    DEFF Research Database (Denmark)

    Johansson, Clara; Møller, Peter; Forchhammer, Lykke;

    2010-01-01

    The increasing use of single cell gel electrophoresis (the comet assay) highlights its popularity as a method for detecting DNA damage, including the use of enzymes for assessment of oxidatively damaged DNA. However, comparison of DNA damage levels between laboratories can be difficult due to...... differences in assay protocols (e.g. lysis conditions, enzyme treatment, the duration of the alkaline treatment and electrophoresis) and in the end points used for reporting results (e.g. %DNA in tail, arbitrary units, tail moment and tail length). One way to facilitate comparisons is to convert primary comet...... assay end points to number of lesions/10(6) bp by calibration with ionizing radiation. The aim of this study was to investigate the inter-laboratory variation in assessment of oxidatively damaged DNA by the comet assay in terms of oxidized purines converted to strand breaks with formamidopyrimidine DNA...

  16. Calibration methods for rotating shadowband irradiometers and evaluation of calibration duration

    Directory of Open Access Journals (Sweden)

    W. Jessen

    2015-10-01

    Full Text Available Resource assessment for Concentrated Solar Power (CSP needs accurate Direct Normal Irradiance (DNI measurements. An option for such measurement campaigns are Rotating Shadowband Irradiometers (RSIs with a thorough calibration. Calibration of RSIs and Si-sensors in general is complex because of the inhomogeneous spectral response of such sensors and incorporates the use of several correction functions. A calibration for a given atmospheric condition and air mass might not work well for a different condition. This paper covers procedures and requirements for two calibration methods for the calibration of Rotating Shadowband Irradiometers. The necessary duration of acquisition of test measurements is examined in regard to the site specific conditions at Plataforma Solar de Almeria (PSA in Spain. Data sets of several long-term calibration periods from PSA are used to evaluate the deviation of results from calibrations with varying duration from the long-term result. The findings show that seasonal changes of environmental conditions are causing small but noticeable fluctuation of calibration results. Certain periods (i.e. November to January and April to May show a higher likelihood of particularly adverse calibration results. These effects can partially be compensated by increasing the inclusions of measurements from outside these periods. Consequently, the duration of calibrations at PSA can now be selected depending on the time of the year in which measurements are commenced.

  17. Linking Diversity and Differentiation

    Directory of Open Access Journals (Sweden)

    Hans-Rolf Gregorius

    2010-03-01

    Full Text Available Generally speaking, the term differentiation refers to differences between collections for the distribution of specified traits of their members, while diversity deals with (effective numbers of trait states (types. Counting numbers of types implies discrete traits such as alleles and genotypes in population genetics or species and taxa in ecology. Comparisons between the concepts of differentiation and diversity therefore primarily refer to discrete traits. Diversity is related to differentiation through the idea that the total diversity of a subdivided collection should be composed of the diversity within the subcollections and a complement called “diversity between subcollections”. The idea goes back to the perception that the mixing of differentiated collections increases diversity. Several existing concepts of “diversity between subcollections” are based on this idea. Among them, β-diversity and fixation (inadvertently called differentiation are the most prominent in ecology and in population genetics, respectively. The pertaining measures are shown to quantify the effect of differentiation in terms of diversity components, though from a dual perspective: the classical perspective of differentiation between collections for their type compositions, and the reverse perspective of differentiation between types for their collection affiliations. A series of measures of diversity-oriented differentiation is presented that consider this dual perspective at two levels of diversity partitioning: the overall type or subcollection diversity and the joint type-subcollection diversity. It turns out that, in contrast with common notions, the measures of fixation (such as FST or GST refer to the perspective of type rather than subcollection differentiation. This unexpected observation strongly suggests that the popular interpretations of fixation measures must be reconsidered.

  18. Controlling variation in the comet assay

    OpenAIRE

    Collins, Andrew R; El Yamani, Naouale; Lorenzo, Yolanda; Shaposhnikov, Sergey; Brunborg, Gunnar; Azqueta, Amaya

    2014-01-01

    Variability of the comet assay is a serious issue, whether it occurs from experiment to experiment in the same laboratory, or between different laboratories analysing identical samples. Do we have to live with high variability, just because the comet assay is a biological assay rather than analytical chemistry? Numerous attempts have been made to limit variability by standardizing the assay protocol, and the critical steps in the assay have been identified; agarose concentration, duration of ...

  19. Updates on the Performance and Calibration of HST/STIS

    Science.gov (United States)

    Lockwood, Sean A.; Debes, John H.; Ely, Justin; Monroe, TalaWanda; Biretta, John A.; De Rosa, Gisella; Fix, Mees; Fox, Andrew; Jedrzejewski, Robert I.; Oliveira, Cristina M.; Peeples, Molly S.; Penton, Steven V.; Plesha, Rachel; Proffitt, Charles R.; Roman-Duval, Julia; Sahnow, David J.; Sonnentrucker, Paule; Taylor, Joanna M.; Walborn, Nolan R.; White, James

    2016-01-01

    The Space Telescope Imaging Spectrograph (STIS) on the Hubble Space Telescope (HST) has been on orbit for almost 19 years and continues to produce high quality scientific results using a diverse complement of operating modes. These include spatially resolved spectroscopy in the UV and optical, high spatial resolution echelle spectroscopy in the UV, and solar-blind imaging in the UV. In addition, STIS possesses unique visible-light coronagraphic modes that keep the instrument at the forefront of exoplanet and debris-disk research. As the instrument's characteristics evolve over the instrument's lifetime, the instrument team at Space Telescope Science Institute monitors its performance and works towards improving the quality of its products. Here we present updates on the status of the STIS CCD and FUV & NUV MAMA detectors, as well as changes to the CalSTIS reduction pipeline and available instrument modes. We present on-going work to calibrate the BAR5 occulter, as well as calibration improvements for the echelle gratings.

  20. Champion Island, Galapagos Stable Oxygen Calibration Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Galapagos Coral Stable Oxygen Calibration Data. Sites: Bartolome Island: 0 deg, 17 min S, 90 deg 33 min W. Champion Island: 1 deg, 15 min S, 90 deg, 05 min W....

  1. Bartolome Island, Galapagos Stable Oxygen Calibration Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Galapagos Coral Stable Oxygen Calibration Data. Sites: Bartolome Island: 0 deg, 17'S, 90 deg 33' W. Champion Island: 1 deg, 15'S, 90 deg, 05' W. Urvina Bay (Isabela...

  2. A transmission calibration method for superconducting resonators

    CERN Document Server

    Cataldo, Giuseppe; Barrentine, Emily M; Brown, Ari D; Moseley, Samuel H; U-Yen, Kongpop

    2014-01-01

    A method is proposed and experimentally explored for \\textit{in-situ} calibration of complex transmission data for superconducting microwave resonators. This cryogenic calibration method accounts for the instrumental transmission response between the vector network analyzer reference plane and the device calibration plane. Once calibrated, the observed resonator response was modeled in detail by two approaches. The first, a phenomenological model based on physically realizable rational functions, enables the extraction of multiple resonance frequencies and widths for coupled resonators without explicit specification of the circuit network. In the second, an ABCD-matrix representation for the distributed transmission line circuit is used to model the observed response from the characteristic impedance and propagation constant. When used in conjunction with electromagnetic simulations, the kinetic inductance fraction can be determined with this method with an accuracy of 2%. Datasets for superconducting microst...

  3. Calibration of a photomultiplier array spectrometer

    Science.gov (United States)

    Bailey, Steven A.; Wright, C. Wayne; Piazza, Charles R.

    1989-01-01

    A systematic approach to the calibration of a photomultiplier array spectrometer is presented. Through this approach, incident light radiance derivation is made by recognizing and tracing gain characteristics for each photomultiplier tube.

  4. Entry tank calibration in TOR pilot plant

    International Nuclear Information System (INIS)

    The objective of this communication is the description of calibration measurements used for determining the uranium and plutonium mass entry in the fast neutron fuel reprocessing pilot plant (TOR) of Marcoule

  5. Calibration of the JEM-EUSO detector

    Directory of Open Access Journals (Sweden)

    Gorodetzky P.

    2013-06-01

    Full Text Available In order to unveil the mystery of ultra high energy cosmic rays (UHECRs, JEM-EUSO (Extreme Universe Space Observatory on-board Japan Experiment Module will observe extensive air showers induced by UHECRs from the International Space Station orbit with a huge acceptance. Calibration of the JEM-EUSO instrument, which consists of Fresnel optics and a focal surface detector with 5000 photomultipliers, is very important to discuss the origin of UHECRs precisely with the observed results. In this paper, the calibration before launch and on-orbit is described. The calibration before flight will be performed as precisely as possible with integrating spheres. In the orbit, the relative change of the performance will be checked regularly with on-board and on-ground light sources. The absolute calibration of photon detection efficiency may be performed with the moon, which is a stable light source in the nature.

  6. Strain Gauges Mounted To Retain Calibration

    Science.gov (United States)

    Butler, Barry L.

    1993-01-01

    Silicon-based semiconductor strain gauges mounted in such way they retain original calibration for several years instead of few months. Improvement effected by bonding gauges to ceramic substrates with glasses instead of epoxies as adhesives.

  7. Calibration of sources for alpha spectroscopy systems

    International Nuclear Information System (INIS)

    This paper describes the calibration methodology for measuring the total alpha activity of plane and thin sources with the Alpha Spectrometer for Silicon Detector in the Nuclear Measures and Dosimetry laboratory at IEAv/CTA. (author)

  8. Absolute calibration technique for spontaneous fission sources

    International Nuclear Information System (INIS)

    An absolute calibration technique for a spontaneously fissioning nuclide (which involves no arbitrary parameters) allows unique determination of the detector efficiency for that nuclide, hence of the fission source strength

  9. Low Power, Self Calibrated Vector Magnetometer Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This Phase I SBIR project investigates a novel approach to vector magnetometry based on high precision measurements of the total magnetic field. The calibration is...

  10. The PREMOS/PICARD instrument calibration

    Science.gov (United States)

    Schmutz, Werner; Fehlmann, André; Hülsen, Gregor; Meindl, Peter; Winkler, Rainer; Thuillier, Gérard; Blattner, Peter; Buisson, François; Egorova, Tatiana; Finsterle, Wolfgang; Fox, Nigel; Gröbner, Julian; Hochedez, Jean-François; Koller, Silvio; Meftah, Mustapha; Meisonnier, Mireille; Nyeki, Stephan; Pfiffner, Daniel; Roth, Hansjörg; Rozanov, Eugene; Spescha, Marcel; Wehrli, Christoph; Werner, Lutz; Wyss, Jules U.

    2009-08-01

    PREMOS is a space experiment scheduled to fly on the French solar mission PICARD. The experiment comprises filter radiometers and absolute radiometers to measure the spectral and total solar irradiance. The aim of PREMOS is to contribute to the long term monitoring of the total solar irradiance, to use irradiance observations for 'nowcasting' the state of the terrestrial middle atmosphere and to provide long term sensitivity calibration for the solar imaging instrument SODISM on PICARD. In this paper we describe the calibration of the instruments. The filter radiometer channels in the visible and near IR were characterized at PMOD/WRC and the UV channels were calibrated at PTB Berlin. The absolute radiometers were compared with the World Radiometric Reference at PMOD/WRC and a power calibration relative to a primary cryogenic radiometer standard was performed in vacuum and air at NPL.

  11. Computer Vision Assisted Virtual Reality Calibration

    Science.gov (United States)

    Kim, W.

    1999-01-01

    A computer vision assisted semi-automatic virtual reality (VR) calibration technology has been developed that can accurately match a virtual environment of graphically simulated three-dimensional (3-D) models to the video images of the real task environment.

  12. Calibration device for ultrasonic inspection equipment

    International Nuclear Information System (INIS)

    The inspection equipment is introduced into the reactor vessel and deposited there. The real inspection of the vessel is performed by means of a manipulator arm carrying an ultrasonic transducer field and being desplaceable along nine axes of motion for the inspection of welds. In order to be sure of adequate testing the origin of the field must exactly be known. This is achieved by means of a calibration unit by which the exact zero position in the vessel is fixed, the position of the ultrasonic transducer is calibrated and its angular position can be determined. It has got a spherical calibration body and at least one inlined reflection surface (tuncated cone surface) that can take a fixed position with respect to the ultrasonic transducer. The calibration body and/or the reflection surface are moved with respect to the ultrasonic transducer until the maximum amplitude of the reflected signal is obtained. (DG)

  13. Low radioactivity spectral gamma calibration facility

    International Nuclear Information System (INIS)

    A low radioactivity calibration facility has been constructed at the Nevada Test Site (NTS). This facility has four calibration models of natural stone that are 3 ft in diameter and 6 ft long, with a 12 in. cored borehole in the center of each model and a lead-shielded run pipe below each model. These models have been analyzed by laboratory natural gamma ray spectroscopy (NGRS) and neutron activation analysis (NAA) for their K, U, and Th content. Also, 42 other elements were analyzed in the NAA. The 222Rn emanation data were collected. Calibrating the spectral gamma tool in this low radioactivity calibration facility allows the spectral gamma log to accurately aid in the recognition and mapping of subsurface stratigraphic units and alteration features associated with unusual concentrations of these radioactive elements, such as clay-rich zones

  14. HPS instrument calibration laboratory accreditation program

    Energy Technology Data Exchange (ETDEWEB)

    Masse, F.X; Eisenhower, E.H.; Swinth, K.L.

    1993-12-31

    The purpose of this paper is to provide an accurate overview of the development and structure of the program established by the Health Physics Society (HPS) for accrediting instrument calibration laboratories relative to their ability to accurately calibrate portable health physics instrumentation. The purpose of the program is to provide radiation protection professionals more meaningful direct and indirect access to the National Institute of Standards and Technology (NIST) national standards, thus introducing a means for improving the uniformity, accuracy, and quality of ionizing radiation field measurements. The process is designed to recognize and document the continuing capability of each accredited laboratory to accurately perform instrument calibration. There is no intent to monitor the laboratory to the extent that each calibration can be guaranteed by the program; this responsibility rests solely with the accredited laboratory.

  15. HPS instrument calibration laboratory accreditation program

    International Nuclear Information System (INIS)

    The purpose of this paper is to provide an accurate overview of the development and structure of the program established by the Health Physics Society (HPS) for accrediting instrument calibration laboratories relative to their ability to accurately calibrate portable health physics instrumentation. The purpose of the program is to provide radiation protection professionals more meaningful direct and indirect access to the National Institute of Standards and Technology (NIST) national standards, thus introducing a means for improving the uniformity, accuracy, and quality of ionizing radiation field measurements. The process is designed to recognize and document the continuing capability of each accredited laboratory to accurately perform instrument calibration. There is no intent to monitor the laboratory to the extent that each calibration can be guaranteed by the program; this responsibility rests solely with the accredited laboratory

  16. The skin-blanching assay.

    Science.gov (United States)

    Smit, P; Neumann, H A M; Thio, H B

    2012-10-01

    The skin-blanching assay is used for the determination and bioequivalence of dermatologic glucocorticoids (GCs). The exact mechanism of the production of blanching is not fully understood, but it is considered that local vasoconstriction of the skin microvasculature and the consequent blood-flow reduction cause this phenomenon. Several factors influence skin blanching, including drug concentration, duration of application, nature of vehicle, occlusion, posture and location. The intensity of vasoconstriction can be measured in several ways: visual or quantitative methods, such as reflectance spectroscopy, thermography, laser Doppler velocimetry and chromametry. In literature, contradicting results in the correlation of the skin-blanching assay with different tests to determine GC sensitivity have been reported, limiting its clinical usefulness.

  17. Comet Assay in Cancer Chemoprevention.

    Science.gov (United States)

    Santoro, Raffaela; Ferraiuolo, Maria; Morgano, Gian Paolo; Muti, Paola; Strano, Sabrina

    2016-01-01

    The comet assay can be useful in monitoring DNA damage in single cells caused by exposure to genotoxic agents, such as those causing air, water, and soil pollution (e.g., pesticides, dioxins, electromagnetic fields) and chemo- and radiotherapy in cancer patients, or in the assessment of genoprotective effects of chemopreventive molecules. Therefore, it has particular importance in the fields of pharmacology and toxicology, and in both environmental and human biomonitoring. It allows the detection of single strand breaks as well as double-strand breaks and can be used in both normal and cancer cells. Here we describe the alkali method for comet assay, which allows to detect both single- and double-strand DNA breaks. PMID:26608293

  18. Nondestructive assay of sale materials

    International Nuclear Information System (INIS)

    This paper covers three primary areas: (1) reasons for performing nondestructive assay on SALE materials; (2) techniques used; and (3) discussion of investigators' revised results. The study shows that nondestructive calorimetric assay of plutonium offers a viable alternative to traditional wet chemical techniques. For these samples, the precision ranged from 0.4 to 0.6% with biases less than 0.2%. Thus, for those materials where sampling errors are the predominant source of uncertainty, this technique can provide improved accuracy and precision while saving time and money as well as reducing the amount of liquid wastes to be handled. In addition, high resolution gamma-ray spectroscopy measurements of solids can provide isotopic analysis data in a cost effective and timely manner. The timeliness of the method can be especially useful to the plant operator for production control and quality control measurements

  19. Automatic colorimetric calibration of human wounds

    Directory of Open Access Journals (Sweden)

    Meert Theo

    2010-03-01

    Full Text Available Abstract Background Recently, digital photography in medicine is considered an acceptable tool in many clinical domains, e.g. wound care. Although ever higher resolutions are available, reproducibility is still poor and visual comparison of images remains difficult. This is even more the case for measurements performed on such images (colour, area, etc.. This problem is often neglected and images are freely compared and exchanged without further thought. Methods The first experiment checked whether camera settings or lighting conditions could negatively affect the quality of colorimetric calibration. Digital images plus a calibration chart were exposed to a variety of conditions. Precision and accuracy of colours after calibration were quantitatively assessed with a probability distribution for perceptual colour differences (dE_ab. The second experiment was designed to assess the impact of the automatic calibration procedure (i.e. chart detection on real-world measurements. 40 Different images of real wounds were acquired and a region of interest was selected in each image. 3 Rotated versions of each image were automatically calibrated and colour differences were calculated. Results 1st Experiment: Colour differences between the measurements and real spectrophotometric measurements reveal median dE_ab values respectively 6.40 for the proper patches of calibrated normal images and 17.75 for uncalibrated images demonstrating an important improvement in accuracy after calibration. The reproducibility, visualized by the probability distribution of the dE_ab errors between 2 measurements of the patches of the images has a median of 3.43 dE* for all calibrated images, 23.26 dE_ab for all uncalibrated images. If we restrict ourselves to the proper patches of normal calibrated images the median is only 2.58 dE_ab! Wilcoxon sum-rank testing (p Conclusion The investigators proposed an automatic colour calibration algorithm that ensures reproducible colour

  20. Protein binding assay for hyaluronate

    Energy Technology Data Exchange (ETDEWEB)

    Lacy, B.E.; Underhill, C.B.

    1986-11-01

    A relatively quick and simple assay for hyaluronate was developed using the specific binding protein, hyaluronectin. The hyaluronectin was obtained by homogenizing the brains of Sprague-Dawley rats, and then centrifuging the homogenate. The resulting supernatant was used as a source of crude hyaluronectin. In the binding assay, the hyaluronectin was mixed with (/sup 3/H)hyaluronate, followed by an equal volume of saturated (NH/sub 4/)/sub 2/SO/sub 4/, which precipitated the hyaluronectin and any (/sup 3/H)hyaluronate associated with it, but left free (/sup 3/H)hyaluronate in solution. The mixture was then centrifuged, and the amount of bound (/sup 3/H)hyaluronate in the precipitate was determined. Using this assay, the authors found that hyaluronectin specifically bound hyaluronate, since other glycosaminoglycans failed to compete for the binding protein. In addition, the interaction between hyaluronectin and hyaluronate was of relatively high affinity, and the size of the hyaluronate did not appear to substantially alter the amount of binding. To determine the amount of hyaluronate in an unknown sample, they used a competition assay in which the binding of a set amount of (/sup 3/H)hyaluronate was blocked by the addition of unlabeled hyaluronate. By comparing the degree of competition of the unknown samples with that of known amounts of hyaluronate, it was possible to determine the amount of hyaluronate in the unknowns. They have found that this method is sensitive to 1 ..mu..g or less of hyaluronate, and is unaffected by the presence of proteins.

  1. Lidar to lidar calibration phase 1

    DEFF Research Database (Denmark)

    Yordanova, Ginka; Courtney, Michael

    This report presents a feasibility study of a lidar to lidar (L2L) calibration procedure. Phase one of the project was conducted at Høvsøre, Denmark. Two windcubes were placed next to the 116m met mast and different methods were applied to obtain the sensing height error of the lidars. The purpose...... is to find the most consistent method and use it in a potential lidar to lidar calibration procedure....

  2. Hydraulic Calibrator for Strain-Gauge Balances

    Science.gov (United States)

    Skelly, Kenneth; Ballard, John

    1987-01-01

    Instrument for calibrating strain-gauge balances uses hydraulic actuators and load cells. Eliminates effects of nonparallelism, nonperpendicularity, and changes of cable directions upon vector sums of applied forces. Errors due to cable stretching, pulley friction, and weight inaccuracy also eliminated. New instrument rugged and transportable. Set up quickly. Developed to apply known loads to wind-tunnel models with encapsulated strain-gauge balances, also adapted for use in calibrating dynamometers, load sensors on machinery and laboratory instruments.

  3. Calibration and monitoring for crystal calorimetry

    CERN Document Server

    Zhu, Ren Yuan

    2005-01-01

    Crystal calorimetry provides excellent energy resolution in high energy and nuclear physics. The light output of heavy crystal scintillators, however, suffers from not negligible damage in radiation environment. A precision calibration and monitoring thus is crucial for maintaining crystal precision in situ. The performance of calibration and monitoring approaches used by BaBar, CLEO and L3 experiments are presented. The design and construction of a laser- based light monitoring system for CMS PWO calorimeter is also discussed.

  4. Automatic Calibration Of Manual Machine Tools

    Science.gov (United States)

    Gurney, Rex D.

    1990-01-01

    Modified scheme uses data from multiple positions and eliminates tedious positioning. Modification of computer program adapts calibration system for convenient use with manually-controlled machine tools. Developed for use on computer-controlled tools. Option added to calibration program allows data on random tool-axis positions to be entered manually into computer for reduction. Instead of setting axis to predetermined positions, operator merely sets it at variety of arbitrary positions.

  5. Virtual Calibration Chamber CPT on Ticino sand

    OpenAIRE

    Butlanska, Joanna; Arroyo Alvarez de Toledo, Marcos; Gens Solé, Antonio

    2010-01-01

    The following paper surnmarizes results of CPT's performed in virtual calibration chamber (VCC) built with a 3D model based on the distinct element method (DEM). A discrete material tailored to mimic Ticino sand is tested at different densities, stress and stress history. The limit cone tip resistance from the numerical experiments shows quantitative agreement with different empirical curves summarizing previous tests on Ticino sand in physical calibration chambers (ENEL and ISMES).

  6. pH sensor calibration procedure

    OpenAIRE

    Artero Delgado, Carola; Nogueras Cervera, Marc; Manuel Lázaro, Antonio; Prat Tasias, Jordi; Prat Farran, Joana d'Arc

    2013-01-01

    This paper describes the calibration of pH sensor located at the OBSEA marine Observatory. This instrument is based on an industrial pH electrode that is connected to a CTD instrument (Conductivity, Temperature, and Depth ). The calibration of the pH sensor has been done using a high precision spectrophotometer pH meter from Institute of Marine Sciences (ICM), and in this way it has been obtained a numerical function for the p H sensor propor...

  7. Calibration of the Super-Kamiokande Detector

    OpenAIRE

    Abe, K.; Hayato, Y.; Iida, T; Iyogi, K.; Kameda, J.; Kishimoto, Y.; Koshio, Y.; Marti, Ll.; Miura, M.; Moriyama, S.; Nakahata, M.(University of Tokyo, Institute for Cosmic Ray Research, Kamioka Observatory, Kamioka, Japan); Nakano, Y.; Nakayama, S.; Obayashi, Y.; Sekiya, H.

    2013-01-01

    Procedures and results on hardware level detector calibration in Super-Kamiokande (SK) are presented in this paper. In particular, we report improvements made in our calibration methods for the experimental phase IV in which new readout electronics have been operating since 2008. The topics are separated into two parts. The first part describes the determination of constants needed to interpret the digitized output of our electronics so that we can obtain physical numbers such as photon count...

  8. A Method to Test Model Calibration Techniques

    Energy Technology Data Exchange (ETDEWEB)

    Judkoff, Ron; Polly, Ben; Neymark, Joel

    2016-08-26

    This paper describes a method for testing model calibration techniques. Calibration is commonly used in conjunction with energy retrofit audit models. An audit is conducted to gather information about the building needed to assemble an input file for a building energy modeling tool. A calibration technique is used to reconcile model predictions with utility data, and then the 'calibrated model' is used to predict energy savings from a variety of retrofit measures and combinations thereof. Current standards and guidelines such as BPI-2400 and ASHRAE-14 set criteria for 'goodness of fit' and assume that if the criteria are met, then the calibration technique is acceptable. While it is logical to use the actual performance data of the building to tune the model, it is not certain that a good fit will result in a model that better predicts post-retrofit energy savings. Therefore, the basic idea here is that the simulation program (intended for use with the calibration technique) is used to generate surrogate utility bill data and retrofit energy savings data against which the calibration technique can be tested. This provides three figures of merit for testing a calibration technique, 1) accuracy of the post-retrofit energy savings prediction, 2) closure on the 'true' input parameter values, and 3) goodness of fit to the utility bill data. The paper will also discuss the pros and cons of using this synthetic surrogate data approach versus trying to use real data sets of actual buildings.

  9. "Calibration-on-the-spot'': How to calibrate an EMCCD camera from its images

    DEFF Research Database (Denmark)

    Mortensen, Kim; Flyvbjerg, Henrik

    In localization-based microscopy, super-resolution is obtained by analyzing isolated diffraction-limited spots imaged, typically, with EMCCD cameras. To compare experiments and calculate localization precision, the photon-to-signal amplification factor is needed but unknown without a calibration...... of the camera. Here we show how this can be done post festum from just a recorded image. We demonstrate this (i) theoretically, mathematically, (ii) by analyzing images recorded with an EMCCD camera, and (iii) by analyzing simulated EMCCD images for which we know the true values of parameters. In summary, our...... method of calibration-on-the-spot allows calibration of a camera with unknown settings from old images on file, with no other info needed. Consequently, calibration-on-the-spot also makes future camera calibrations before and after measurements unnecessary, because the calibration is encoded in recorded...

  10. Fluorescence Polarization Assays in Small Molecule Screening

    Science.gov (United States)

    Lea, Wendy A.; Simeonov, Anton

    2011-01-01

    Importance of the field Fluorescence polarization (FP) is a homogeneous method that allows rapid and quantitative analysis of diverse molecular interactions and enzyme activities. This technique has been widely utilized in clinical and biomedical settings, including the diagnosis of certain diseases and monitoring therapeutic drug levels in body fluids. Recent developments in the field has been symbolized by the facile adoption of FP in high-throughput screening (HTS) and small molecule drug discovery of an increasing range of target classes. Areas covered in this review The article provides a brief overview on the theoretical foundation of FP, followed by updates on recent advancements in its application for various drug target classes, including G-protein coupled receptors (GPCRs), enzymes and protein-protein interactions (PPIs). The strengths and weaknesses of this method, practical considerations in assay design, novel applications, and future directions are also discussed. What the reader will gain The reader will be informed of the most recent advancements and future directions of FP application to small molecule screening. Take home message In addition to its continued utilization in high-throughput screening, FP has expanded into new disease and target areas and has been marked by increased use of labeled small molecule ligands for receptor binding studies. PMID:22328899

  11. Revised landsat-5 thematic mapper radiometric calibration

    Science.gov (United States)

    Chander, G.; Markham, B.L.; Barsi, J.A.

    2007-01-01

    Effective April 2, 2007, the radiometric calibration of Landsat-5 (L5) Thematic Mapper (TM) data that are processed and distributed by the U.S. Geological Survey (USGS) Center for Earth Resources Observation and Science (EROS) will be updated. The lifetime gain model that was implemented on May 5, 2003, for the reflective bands (1-5, 7) will be replaced by a new lifetime radiometric-calibration curve that is derived from the instrument's response to pseudoinvariant desert sites and from cross calibration with the Landsat-7 (L7) Enhanced TM Plus (ETM+). Although this calibration update applies to all archived and future L5 TM data, the principal improvements in the calibration are for the data acquired during the first eight years of the mission (1984-1991), where the changes in the instrument-gain values are as much as 15%. The radiometric scaling coefficients for bands 1 and 2 for approximately the first eight years of the mission have also been changed. Users will need to apply these new coefficients to convert the calibrated data product digital numbers to radiance. The scaling coefficients for the other bands have not changed. ?? 2007 IEEE.

  12. Calibration of the Hydrological Simulation Program Fortran (HSPF) model using automatic calibration and geographical information systems

    Science.gov (United States)

    Al-Abed, N. A.; Whiteley, H. R.

    2002-11-01

    Calibrating a comprehensive, multi-parameter conceptual hydrological model, such as the Hydrological Simulation Program Fortran model, is a major challenge. This paper describes calibration procedures for water-quantity parameters of the HSPF version 10·11 using the automatic-calibration parameter estimator model coupled with a geographical information system (GIS) approach for spatially averaged properties. The study area was the Grand River watershed, located in southern Ontario, Canada, between 79° 30 and 80° 57W longitude and 42° 51 and 44° 31N latitude. The drainage area is 6965 km2. Calibration efforts were directed to those model parameters that produced large changes in model response during sensitivity tests run prior to undertaking calibration. A GIS was used extensively in this study. It was first used in the watershed segmentation process. During calibration, the GIS data were used to establish realistic starting values for the surface and subsurface zone parameters LZSN, UZSN, COVER, and INFILT and physically reasonable ratios of these parameters among watersheds were preserved during calibration with the ratios based on the known properties of the subwatersheds determined using GIS. This calibration procedure produced very satisfactory results; the percentage difference between the simulated and the measured yearly discharge ranged between 4 to 16%, which is classified as good to very good calibration. The average simulated daily discharge for the watershed outlet at Brantford for the years 1981-85 was 67 m3 s-1 and the average measured discharge at Brantford was 70 m3 s-1. The coupling of a GIS with automatice calibration produced a realistic and accurate calibration for the HSPF model with much less effort and subjectivity than would be required for unassisted calibration.

  13. NuSTAR ground calibration: The Rainwater Memorial Calibration Facility (RaMCaF)

    DEFF Research Database (Denmark)

    Brejnholt, Nicolai; Christensen, Finn Erland; Jakobsen, Anders Clemen;

    2011-01-01

    The Nuclear Spectroscopic Telescope Array (NuSTAR) is a NASA Small Explorer mission that will carry the first focusing hard X-ray (5-80 keV ) telescope to orbit. The ground calibration of the three flight optics was carried out at the Rainwater Memorial Calibration Facility (RaMCaF) built for this...... purpose. In this article we present the facility and its use for the ground calibration of the three optics....

  14. Is Your System Calibrated? MRI Gradient System Calibration for Pre-Clinical, High-Resolution Imaging

    OpenAIRE

    James O'Callaghan; Jack Wells; Simon Richardson; Holly Holmes; Yichao Yu; Simon Walker-Samuel; Bernard Siow; Lythgoe, Mark F

    2014-01-01

    High-field, pre-clinical MRI systems are widely used to characterise tissue structure and volume in small animals, using high resolution imaging. Both applications rely heavily on the consistent, accurate calibration of imaging gradients, yet such calibrations are typically only performed during maintenance sessions by equipment manufacturers, and potentially with acceptance limits that are inadequate for phenotyping. To overcome this difficulty, we present a protocol for gradient calibration...

  15. Correlation between the genotoxicity endpoints measured by two different genotoxicity assays: comet assay and CBMN assay

    Directory of Open Access Journals (Sweden)

    Carina Ladeira

    2015-06-01

    The use of the CBMN assay in in vitro genetic toxicology testing is well established and in fact it has become an accepted standard method to assess the genotoxic hazard of chemicals which led to the development of a special guideline by the Organization for Economic Cooperation and Development (OECD, the OECD 487 guideline (Kirsch-Volders et al., 2014. The CBMN assay is an effective tool for the study of cellular and nuclear dysfunction caused by in vitro or in vivo aging, micronutrient deficiency or excess, genotoxins exposure and genetic defects in genome maintenance. It is also fruitful in the emerging fields of nutrigenomics and toxicogenomics and their combinations, as it becomes increasingly clear that nutrient status also impacts on sensitivity to exogenous genotoxins (Fenech, 2005, 2007. Many results obtained by this assay indicate the potential predictive value of the CBMN assay with respect to cancer risk and validate its use as a test for detecting nutritional, environmental and genetic factors that are potentially carcinogenic. Also it is used by pharmaceutical industry, human biomonitoring of genotoxic exposures and its increasing application in preventive medicine and nutrition and the increased investment in the automation of the CBMN assay are indicative of the increasing importance of this test (Fenech, 2007. The comet assay or single-cell gel electrophoresis (SCGE is a simple, sensitive method for detecting DNA-strand breaks. Cells embedded in agarose on a microscope slide are lysed with detergent and 2.5 M NaCl and fresh Triton X-100 to remove membranes and soluble cell constituents, including most histones, leaving the DNA, still supercoiled and attached to a nuclear matrix, as a nucleoid. A break in one strand of a DNA loop is enough to release the supercoiling, and during electrophoresis the relaxed loops are able to extend towards the anode (Fairbairn et al., 1995; Collins et al., 1997; Moller et al., 2000; Azqueta et al., 2009; Collins

  16. Contributions of the SDR Task Network tool to Calibration and Validation of the NPOESS Preparatory Project instruments

    Science.gov (United States)

    Feeley, J.; Zajic, J.; Metcalf, A.; Baucom, T.

    2009-12-01

    The National Polar-orbiting Operational Environmental Satellite System (NPOESS) Preparatory Project (NPP) Calibration and Validation (Cal/Val) team is planning post-launch activities to calibrate the NPP sensors and validate Sensor Data Records (SDRs). The IPO has developed a web-based data collection and visualization tool in order to effectively collect, coordinate, and manage the calibration and validation tasks for the OMPS, ATMS, CrIS, and VIIRS instruments. This tool is accessible to the multi-institutional Cal/Val teams consisting of the Prime Contractor and Government Cal/Val leads along with the NASA NPP Mission team, and is used for mission planning and identification/resolution of conflicts between sensor activities. Visualization techniques aid in displaying task dependencies, including prerequisites and exit criteria, allowing for the identification of a critical path. This presentation will highlight how the information is collected, displayed, and used to coordinate the diverse instrument calibration/validation teams.

  17. Energy calibration issues in nuclear resonant vibrational spectroscopy: observing small spectral shifts and making fast calibrations.

    Science.gov (United States)

    Wang, Hongxin; Yoda, Yoshitaka; Dong, Weibing; Huang, Songping D

    2013-09-01

    The conventional energy calibration for nuclear resonant vibrational spectroscopy (NRVS) is usually long. Meanwhile, taking NRVS samples out of the cryostat increases the chance of sample damage, which makes it impossible to carry out an energy calibration during one NRVS measurement. In this study, by manipulating the 14.4 keV beam through the main measurement chamber without moving out the NRVS sample, two alternative calibration procedures have been proposed and established: (i) an in situ calibration procedure, which measures the main NRVS sample at stage A and the calibration sample at stage B simultaneously, and calibrates the energies for observing extremely small spectral shifts; for example, the 0.3 meV energy shift between the 100%-(57)Fe-enriched [Fe4S4Cl4](=) and 10%-(57)Fe and 90%-(54)Fe labeled [Fe4S4Cl4](=) has been well resolved; (ii) a quick-switching energy calibration procedure, which reduces each calibration time from 3-4 h to about 30 min. Although the quick-switching calibration is not in situ, it is suitable for normal NRVS measurements.

  18. Correlation between the genotoxicity endpoints measured by two different genotoxicity assays: comet assay and CBMN assay

    OpenAIRE

    Carina Ladeira; Susana Viegas; Gomes, Manuel C.

    2015-01-01

    The cytokinesis-block micronucleus cytome (CBMN) assay is a comprehensive system for measuring DNA damage; cytostasis and cytotoxicity-DNA damage events are scored specifically in once-divided binucleated cells. The endpoints possible to be measured are micronuclei (MN), a biomarker of chromosome breakage and/or whole chromosome loss, nucleoplasmic bridges (NPB), a biomarker of DNA misrepair and/or telomere end-fusions, and nuclear buds (NBUD), a biomarker of elimination of amplified DNA and/...

  19. Fluorescence polarization assays in high-throughput screening and drug discovery: a review

    Science.gov (United States)

    Hall, Matthew D.; Yasgar, Adam; Peryea, Tyler; Braisted, John C.; Jadhav, Ajit; Simeonov, Anton; Coussens, Nathan P.

    2016-06-01

    The sensitivity of fluorescence polarization (FP) and fluorescence anisotropy (FA) to molecular weight changes has enabled the interrogation of diverse biological mechanisms, ranging from molecular interactions to enzymatic activity. Assays based on FP/FA technology have been widely utilized in high-throughput screening (HTS) and drug discovery due to the homogenous format, robust performance and relative insensitivity to some types of interferences, such as inner filter effects. Advancements in assay design, fluorescent probes, and technology have enabled the application of FP assays to increasingly complex biological processes. Herein we discuss different types of FP/FA assays developed for HTS, with examples to emphasize the diversity of applicable targets. Furthermore, trends in target and fluorophore selection, as well as assay type and format, are examined using annotated HTS assays within the PubChem database. Finally, practical considerations for the successful development and implementation of FP/FA assays for HTS are provided based on experience at our center and examples from the literature, including strategies for flagging interference compounds among a list of hits.

  20. Standard test method for nondestructive assay of radioactive material by tomographic gamma scanning

    CERN Document Server

    American Society for Testing and Materials. Philadelphia

    2010-01-01

    1.1 This test method describes the nondestructive assay (NDA) of gamma ray emitting radionuclides inside containers using tomographic gamma scanning (TGS). High resolution gamma ray spectroscopy is used to detect and quantify the radionuclides of interest. The attenuation of an external gamma ray transmission source is used to correct the measurement of the emission gamma rays from radionuclides to arrive at a quantitative determination of the radionuclides present in the item. 1.2 The TGS technique covered by the test method may be used to assay scrap or waste material in cans or drums in the 1 to 500 litre volume range. Other items may be assayed as well. 1.3 The test method will cover two implementations of the TGS procedure: (1) Isotope Specific Calibration that uses standards of known radionuclide masses (or activities) to determine system response in a mass (or activity) versus corrected count rate calibration, that applies to only those specific radionuclides for which it is calibrated, and (2) Respo...

  1. Teaching Diverse Learners. Diversities in the Classroom.

    Science.gov (United States)

    Glazer, Susan Mandel

    1996-01-01

    Describes "diverse" as a socially acceptable term for both gifted children and at-risk children. Recommends describing children's specific behavior to create a more definitive picture. Includes example of observation of a "dysgraphic" child and the specific behaviors expressed, suggesting that results of observation can yield ideas about…

  2. Design of standards for nondestructive assay of special nuclear material

    Energy Technology Data Exchange (ETDEWEB)

    Smith, H.A. Jr.; Stewart, J.E. [Los Alamos National Lab., NM (United States); Ruhter W. [Lawrence Livermore National Lab., CA (United States)

    1997-05-01

    Nondestructive assay (NDA) of special nuclear material (SNM) involves a variety of measurement techniques, instruments, and nuclear materials. High-quality measurements require well-characterized SNM standards that represent the expected range of mass, chemical composition, and physical properties of the SNM to be measured. Due to the very limited commercial availability of NDA standards, facilities must usually produce their own standards, both to meet their specific measurement needs and to comply with existing regulations. This paper will describe the current extent to which NDA standards are commercially available. The authors will further describe the types of NDA standards used to calibrate and verify the measurement techniques commonly used in the safeguards of SNM. Several types of NDA standards will be discussed in detail to illustrate the considerations that go into specifying and designing traceable, representative standards for materials accounting measurements.

  3. Calibration Of Partial-Pressure-Of-Oxygen Sensors

    Science.gov (United States)

    Yount, David W.; Heronimus, Kevin

    1995-01-01

    Report and analysis of, and discussion of improvements in, procedure for calibrating partial-pressure-of-oxygen sensors to satisfy Spacelab calibration requirements released. Sensors exhibit fast drift, which results in short calibration period not suitable for Spacelab. By assessing complete process of determining total drift range available, calibration procedure modified to eliminate errors and still satisfy requirements without compromising integrity of system.

  4. The ISO SWS Calibration: Strategy and Lessons Learned

    NARCIS (Netherlands)

    Roelfsema, P. R.; Valentijn, E. A.; Bauer, O. H.; Beintema, D. A.; Boxhoorn, D. R.; Feuchtgruber, H.; de Graauw, T.; Heras, A. M.; Huygen, R.; Kester, D. J. M.; Lahuis, F.; Leech, K.; Lorente, R.; Morris, P. W.; Salama, A.; Schaeidt, S. G.; Shipman, R. F.; Vandenbussche, B.; Weiprecht, E.

    2003-01-01

    Instrument calibration can be seen as having several different components; clearly there is the use of specific calibration tools according to specific procedures on well defined data sets to yield calibration parameters. Also the application of such calibration parameters to data is part of the gen

  5. 21 CFR 874.1080 - Audiometer calibration set.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Audiometer calibration set. 874.1080 Section 874...) MEDICAL DEVICES EAR, NOSE, AND THROAT DEVICES Diagnostic Devices § 874.1080 Audiometer calibration set. (a) Identification. An audiometer calibration set is an electronic reference device that is intended to calibrate...

  6. Enzyme-linked immunosorbent assay characterization of basal variation and heritability of systemic microfibrillar-associated protein 4.

    Directory of Open Access Journals (Sweden)

    Susanne Gjørup Sækmose

    Full Text Available BACKGROUND: Microfibrillar-associated protein 4 (MFAP4 is a systemic biomarker that is significantly elevated in samples from patients suffering from hepatic cirrhosis. The protein is generally localized to elastic fibers and other connective tissue fibers in the extracellular matrix (ECM, and variation in systemic MFAP4 (sMFAP4 has the potential to reflect diverse diseases with increased ECM turnover. Here, we aimed to validate an enzyme-linked immunosorbent assay (ELISA for the measurement of sMFAP4 with an emphasis on the robustness of the assay. Moreover, we aimed to determine confounders influencing the basal sMFAP4 variability and the genetic contribution to the basal variation. METHODS: The sandwich ELISA was based on two monoclonal anti-MFAP4 antibodies and was optimized and calibrated with a standard of recombinant MFAP4. The importance of pre-analytical sample handling was evaluated regarding sample tube type, time, and temperature conditions. The mean value structure and variance structure was determined in a twin cohort including 1,417 Danish twins (age 18-67 years by mixed-effect linear regression modeling. RESULTS: The practical working range of the sandwich ELISA was estimated to be 4-75 U/ml. The maximum intra- and inter-assay variation was estimated to be 8.7% and 6.6%, respectively. Sample handling and processing appeared to influence MFAP4 measurements only marginally. The average concentration of sMFAP4 in the serum was 18.9 ± 8.4 (SD U/ml in the twin cohort (95% CI: 18.5-19.4, median sMFAP4 17.3 U/ml. The mean structure model was demonstrated to include waist-hip ratio, age, and cigarette smoking status in interactions with gender. A relatively low heritability of h(2 = 0.24 was found after applying a model including additive genetic factors and shared and non-shared environmental factors. CONCLUSIONS: The described ELISA provides robust measures of the liver fibrosis marker sMFAP4. The low heritability and the relatively

  7. Putting Diversity to Work

    DEFF Research Database (Denmark)

    Holck, Lotte

    2016-01-01

    Purpose: The purpose of this article is to critically explore why a diversity agenda in favor of equal opportunities failed despite apparent organizational support and commitment to diversity. Design/methodology/approach: Drawing on data from a municipal center, this study inquires into how organ...

  8. An Algorithmic Diversity Diet?

    DEFF Research Database (Denmark)

    Sørensen, Jannick Kirk; Schmidt, Jan-Hinrik

    2016-01-01

    With the growing influence of personalized algorithmic recommender systems on the exposure of media content to users, the relevance of discussing the diversity of recommendations increases, particularly as far as public service media (PSM) is concerned. An imagined implementation of a diversity d...

  9. Equality, Innovation and Diversity.

    Science.gov (United States)

    Smith, Janet

    1999-01-01

    Offers some ideas concerning promotion of gender equality and diversity within European Union-funded programs and activities. Reviews efforts since the 1970s to foster equal access in European schools and universities, examines some principles of innovation and entrepreneurship, and considers stages in diversity policy development. (DB)

  10. Evolution & Diversity in Plants.

    Science.gov (United States)

    Pearson, Lorentz C.

    1988-01-01

    Summarizes recent findings that help in understanding how evolution has brought about the diversity of plant life that presently exists. Discusses basic concepts of evolution, diversity and classification, the three-line hypothesis of plant evolution, the origin of fungi, and the geologic time table. Included are 31 references. (CW)

  11. Species diversity modulates predation

    NARCIS (Netherlands)

    Kratina, P.; Vos, M.; Anholt, B.R.

    2007-01-01

    Predation occurs in a context defined by both prey and non-prey species. At present it is largely unknown how species diversity in general, and species that are not included in a predator's diet in particular, modify predator–prey interactions.Therefore we studied how both the density and diversity

  12. Global Diversity and Leadership.

    Science.gov (United States)

    Ruiz, Art

    2003-01-01

    Argues that global diversity has become a business imperative in today's business climate. Global diversity is of core importance even for companies that are considered domestic. Suggests community colleges need help in understanding their customer base and their shifting values in order to meet their needs and win customer loyalty. (NB)

  13. Issue Brief on Diversity

    Science.gov (United States)

    Division on Developmental Disabilities, Council for Exceptional Children (NJ1), 2013

    2013-01-01

    During the past year, the Diversity Committee of the Division of Developmental Disabilities (DDD) Board worked with the Board and the Issues Committee Chair to develop an issue brief addressing diversity, its impact on the membership and the wider community that is served by the work of DDD, resulting in recommendations that will influence policy…

  14. Calibration Matters: Advances in Strapdown Airborne Gravimetry

    Science.gov (United States)

    Becker, D.

    2015-12-01

    Using a commercial navigation-grade strapdown inertial measurement unit (IMU) for airborne gravimetry can be advantageous in terms of cost, handling, and space consumption compared to the classical stable-platform spring gravimeters. Up to now, however, large sensor errors made it impossible to reach the mGal-level using such type IMUs as they are not designed or optimized for this kind of application. Apart from a proper error-modeling in the filtering process, specific calibration methods that are tailored to the application of aerogravity may help to bridge this gap and to improve their performance. Based on simulations, a quantitative analysis is presented on how much IMU sensor errors, as biases, scale factors, cross couplings, and thermal drifts distort the determination of gravity and the deflection of the vertical (DOV). Several lab and in-field calibration methods are briefly discussed, and calibration results are shown for an iMAR RQH unit. In particular, a thermal lab calibration of its QA2000 accelerometers greatly improved the long-term drift behavior. Latest results from four recent airborne gravimetry campaigns confirm the effectiveness of the calibrations applied, with cross-over accuracies reaching 1.0 mGal (0.6 mGal after cross-over adjustment) and DOV accuracies reaching 1.1 arc seconds after cross-over adjustment.

  15. Evolution of Altimetry Calibration and Future Challenges

    Science.gov (United States)

    Fu, Lee-Lueng; Haines, Bruce J.

    2012-01-01

    Over the past 20 years, altimetry calibration has evolved from an engineering-oriented exercise to a multidisciplinary endeavor driving the state of the art. This evolution has been spurred by the developing promise of altimetry to capture the large-scale, but small-amplitude, changes of the ocean surface containing the expression of climate change. The scope of altimeter calibration/validation programs has expanded commensurately. Early efforts focused on determining a constant range bias and verifying basic compliance of the data products with mission requirements. Contemporary investigations capture, with increasing accuracies, the spatial and temporal characteristics of errors in all elements of the measurement system. Dedicated calibration sites still provide the fundamental service of estimating absolute bias, but also enable long-term monitoring of the sea-surface height and constituent measurements. The use of a network of island and coastal tide gauges has provided the best perspective on the measurement stability, and revealed temporal variations of altimeter measurement system drift. The cross-calibration between successive missions provided fundamentally new information on the performance of altimetry systems. Spatially and temporally correlated errors pose challenges for future missions, underscoring the importance of cross-calibration of new measurements against the established record.

  16. WFC3: Improved WFC3 Calibration Products

    Science.gov (United States)

    Gunning, Heather C.; Sosey, M. L.; Anderson, J.; Lee, J. C.; Pirzkal, N.; MacKenty, J. W.; Kozhurina-Platais, V.; Deustua, S. E.; Hammer, D.; Dahlen, T.; Sabbi, E.; Mack, J.; Baggett, S. M.; WFC3 Team

    2014-01-01

    The Wide Field Camera 3 (WFC3) is a fourth-generation UV/visible and IR imaging instrument on the Hubble Space Telescope (HST). Installed in May 2009, during HST servicing mission 4, both channels have been performing very well on-orbit. To provide optimum calibrated data, the WFC3 team routinely updates and refines the calibration software and associated files, designated as calibration products. We present some of the recently improved calibration products that will be of interest to current and future users of WFC3, including information on the chip-dependent zeropoints and flat fields, post-flash calibrations, and detector-to-image distortion corrections. The latter results in four new extensions (two per chip and dimension), in all UVIS FLTs retrieved from MAST after September 10, 2013. The D2IMFILE contains astrometric corrections for shifts of the raw X and Y positions induced by the lithographic-mask pattern. We discuss the migration of CALWF3 from the STSDAS package to HSTCAL, a package independent of IRAF; as a consequence, the IRAF/STSDAS version of CALWF3 is no longer being updated. Finally, we summarize recent improvements to aXe, a PyRAF/IRAF software package that enables automated extraction of spectra from WFC3 slitless spectral (grism) images. Updated versions of aXe are made available as part of the STSDAS testing environment (SSBX).

  17. PERSONALISED BODY COUNTER CALIBRATION USING ANTHROPOMETRIC PARAMETERS.

    Science.gov (United States)

    Pölz, S; Breustedt, B

    2016-09-01

    Current calibration methods for body counting offer personalisation for lung counting predominantly with respect to ratios of body mass and height. Chest wall thickness is used as an intermediate parameter. This work revises and extends these methods using a series of computational phantoms derived from medical imaging data in combination with radiation transport simulation and statistical analysis. As an example, the method is applied to the calibration of the In Vivo Measurement Laboratory (IVM) at Karlsruhe Institute of Technology (KIT) comprising four high-purity germanium detectors in two partial body measurement set-ups. The Monte Carlo N-Particle (MCNP) transport code and the Extended Cardiac-Torso (XCAT) phantom series have been used. Analysis of the computed sample data consisting of 18 anthropometric parameters and calibration factors generated from 26 photon sources for each of the 30 phantoms reveals the significance of those parameters required for producing an accurate estimate of the calibration function. Body circumferences related to the source location perform best in the example, while parameters related to body mass show comparable but lower performances, and those related to body height and other lengths exhibit low performances. In conclusion, it is possible to give more accurate estimates of calibration factors using this proposed approach including estimates of uncertainties related to interindividual anatomical variation of the target population. PMID:26396263

  18. Adaptable Multivariate Calibration Models for Spectral Applications

    Energy Technology Data Exchange (ETDEWEB)

    THOMAS,EDWARD V.

    1999-12-20

    Multivariate calibration techniques have been used in a wide variety of spectroscopic situations. In many of these situations spectral variation can be partitioned into meaningful classes. For example, suppose that multiple spectra are obtained from each of a number of different objects wherein the level of the analyte of interest varies within each object over time. In such situations the total spectral variation observed across all measurements has two distinct general sources of variation: intra-object and inter-object. One might want to develop a global multivariate calibration model that predicts the analyte of interest accurately both within and across objects, including new objects not involved in developing the calibration model. However, this goal might be hard to realize if the inter-object spectral variation is complex and difficult to model. If the intra-object spectral variation is consistent across objects, an effective alternative approach might be to develop a generic intra-object model that can be adapted to each object separately. This paper contains recommendations for experimental protocols and data analysis in such situations. The approach is illustrated with an example involving the noninvasive measurement of glucose using near-infrared reflectance spectroscopy. Extensions to calibration maintenance and calibration transfer are discussed.

  19. Polarimetric PALSAR System Model Assessment and Calibration

    Science.gov (United States)

    Touzi, R.; Shimada, M.

    2009-04-01

    Polarimetric PALSAR system parameters are assessed using data sets collected over various calibration sites. The data collected over the Amazonian forest permits validating the zero Faraday rotation hypotheses near the equator. The analysis of the Amazonian forest data and the response of the corner reflectors deployed during the PALSAR acquisitions lead to the conclusion that the antenna is highly isolated (better than -35 dB). Theses results are confirmed using data collected over the Sweden and Ottawa calibration sites. The 5-m height trihedrals deployed in the Sweden calibration site by the Chalmers University of technology permits accurate measurement of antenna parameters, and detection of 2-3 degree Faraday rotation during day acquisition, whereas no Faraday rotation was noted during night acquisition. Small Faraday rotation angles (2-3 degree) have been measured using acquisitions over the DLR Oberpfaffenhofen and the Ottawa calibration sites. The presence of small but still significant Faraday rotation (2-3 degree) induces a CR return at the crosspolarization HV and VH that should not be interpreted as the actual antenna cross-talk. PALSAR antenna is highly isolated (better than -35 dB), and diagonal antenna distortion matrices (with zero cross-talk terms) can be used for accurate calibration of PALSAR polarimetric data.

  20. Numerical calibration of a Lorentz force flowmeter

    International Nuclear Information System (INIS)

    Numerical simulation of complex-shaped devices for contactless electromagnetic flow measurement in metallurgy is a challenge for computational magnetohydrodynamics. We report a series of numerical simulations which demonstrate for the first time that it is possible to predict the calibration constant of a generic Lorentz force flowmeter (LFF) with an uncertainty close to the requirements of real-life industrial applications. Our simulations involve both magnetostatic computations of a complex-shaped magnet system and magnetohydrodynamic computations of the flow of a liquid metal in a nozzle under the influence of a predominantly transverse magnetic field. In order to assess the role of turbulence, the simulations have been performed both for laminar and for turbulent flows using Reynolds-averaged Navier–Stokes equations in the latter case. In addition to the numerical simulations we have measured the calibration constant of the considered LFF using room-temperature liquid metal instead of liquid aluminum. A comparison between the numerically predicted and the measured values of the calibration constant shows that they differ by only 3.4%. This result suggests that numerical calibration of a LFF may become an economic alternative to expensive full-scale experimental calibration. (paper)

  1. SCIAMACHY Level 1 data: calibration concept and in-flight calibration

    NARCIS (Netherlands)

    Lichtenberg, G.; Kleipool, Q.; Krijger, J.M.; Soest, G. van; Hees, R. van; Tilstra, L.G.; Acarreta, J.R.; Aben, I.; Ahlers, B.; Bovensmann, H.; Chance, K.; Gloudemans, A.M.S.; Hoogeveen, R.W.M.; Jongma, R.T.N.; Noël, S.; Piters, A.; Schrijver, H.; Schrijvers, C.; Sioris, C.E.; Skupin, J.; Slijkhuis, S.; Stammes, P.; Wuttke, M.

    2006-01-01

    The calibration of SCIAMACHY was thoroughly checked since the instrument was launched on-board ENVISAT in February 2002. While SCIAMACHY's functional performance is excellent since launch, a number of technical difficulties have appeared, that required adjustments to the calibration. The problems ca

  2. Lipase production by diverse phylogenetic clades of Aureobasidium pullulans

    Science.gov (United States)

    Thirty-nine strains representing 12 diverse phylogenetic clades of Aureobasidium pullulans were surveyed for lipase production using a quantitative assay. Strains in clades 4 and 10 produced 0.2-0.3 U lipase/ml, while color variant strain NRRL Y-2311-1 in clade 8 produced 0.54 U lipase/ml. Strains i...

  3. Embracing cultural diversity.

    Science.gov (United States)

    Casady, W M

    2001-01-01

    Healthcare providers from all backgrounds are taught the Western medicine approach with little consideration given to cultural-specific care. Yet, today it is difficult to ignore that approximately 33 percent of Americans originate from ethnically diverse groups. As our population continues to become more diversified, it is imperative that healthcare professionals become more sensitive to cultural differences. Effectively managing cultural diversity in the workplace requires a complex set of skills as well as an understanding of the concept. Communication skills will be challenged in a complex and diverse work environment. Managers must learn to listen. Embracing cultural diversity is a two-step process. The first step begins with personal self-interest and self-examination. The second step in the process is the "awakening." Tomorrow's successful managers will take an active role today in creating an environment that views diversity as an asset to the work force. PMID:11302066

  4. Managing Protean Diversity

    DEFF Research Database (Denmark)

    Marfelt, Mikkel Mouritz; Muhr, Sara Louise

    2016-01-01

    . In this article, we follow the call for critically investigating the contexts influencing diversity management by analyzing the development of a global human resource management project initiated to promote a culturally diverse workforce. We find that despite good intentions, as well as support from the top......Recently, global workforce diversity and its management have received criticism for not paying attention to the contextual influence stemming from socially constructed dialectics of power and politics. These contextual dynamics, however, tend to be viewed as external to the organization...... management, the project dissolves through micropolitics and power dynamics. We contribute to the critical literature on workforce diversity by identifying how organizational contextual dynamics influence the way the concept of workforce diversity is constructed and understood at work. Based on these findings...

  5. Serum indices: managing assay interference.

    Science.gov (United States)

    Farrell, Christopher-John L; Carter, Andrew C

    2016-09-01

    Clinical laboratories frequently encounter samples showing significant haemolysis, icterus or lipaemia. Technical advances, utilizing spectrophotometric measurements on automated chemistry analysers, allow rapid and accurate identification of such samples. However, accurate quantification of haemolysis, icterus and lipaemia interference is of limited value if laboratories do not set rational alert limits, based on sound interference testing experiments. Furthermore, in the context of increasing consolidation of laboratories and the formation of laboratory networks, there is an increasing requirement for harmonization of the handling of haemolysis, icterus and lipaemia-affected samples across different analytical platforms. Harmonization may be best achieved by considering both the analytical aspects of index measurement and the possible variations in the effects of haemolysis, icterus and lipaemia interferences on assays from different manufacturers. Initial verification studies, followed up with ongoing quality control testing, can help a laboratory ensure the accuracy of haemolysis, icterus and lipaemia index results, as well as assist in managing any biases in index results from analysers from different manufacturers. Similarities, and variations, in the effect of haemolysis, icterus and lipaemia interference in assays from different manufacturers can often be predicted from the mechanism of interference. Nevertheless, interference testing is required to confirm expected similarities or to quantify differences. It is important that laboratories are familiar with a number of interference testing protocols and the particular strengths and weaknesses of each. A rigorous approach to all aspects of haemolysis, icterus and lipaemia interference testing allows the analytical progress in index measurement to be translated into improved patient care. PMID:27147624

  6. SNO+ Scintillator Purification and Assay

    Science.gov (United States)

    Ford, R.; Chen, M.; Chkvorets, O.; Hallman, D.; Vázquez-Jáuregui, E.

    2011-04-01

    We describe the R&D on the scintillator purification and assay methods and technology for the SNO+ neutrino and double-beta decay experiment. The SNO+ experiment is a replacement of the SNO heavy water with liquid scintillator comprised of 2 g/L PPO in linear alkylbenzene (LAB). During filling the LAB will be transported underground by rail car and purified by multi-stage distillation and steam stripping at a flow rate of 19 LPM. While the detector is operational the scintillator can be recirculated at 150 LPM (full detector volume in 4 days) to provide repurification as necessary by either water extraction (for Ra, K, Bi) or by functional metal scavenger columns (for Pb, Ra, Bi, Ac, Th) followed by steam stripping to remove noble gases and oxygen (Rn, O2, Kr, Ar). The metal scavenger columns also provide a method for scintillator assay for ex-situ measurement of the U and Th chain radioactivity. We have developed "natural" radioactive spikes of Pb and Ra in LAB and use these for purification testing. Lastly, we present the planned operating modes and purification strategies and the plant specifications and design.

  7. Proteasome Assay in Cell Lysates

    Science.gov (United States)

    Maher, Pamela

    2016-01-01

    The ubiquitin-proteasome system (UPS) mediates the majority of the proteolysis seen in the cytoplasm and nucleus of mammalian cells. As such it plays an important role in the regulation of a variety of physiological and pathophysiological processes including tumorigenesis, inflammation and cell death (Ciechanover, 2005; Kisselev and Goldberg, 2001). A number of recent studies have shown that proteasome activity is decreased in a variety of neurological disorders including Parkinson's disease, Alzheimer's disease, amyotrophic lateral sclerosis and stroke as well as during normal aging (Chung et al., 2001; Ciechanover and Brundin, 2003; Betarbet et al., 2005). This decrease in proteasome activity is thought to play a critical role in the accumulation of abnormal and oxidized proteins. Protein clearance by the UPS involves two sequential reactions. The first is the tagging of protein lysine residues with ubiquitin (Ub) and the second is the subsequent degradation of the tagged proteins by the proteasome. We herein describe an assay for the second of these two reactions (Valera et al., 2013). This assay uses fluorogenic substrates for each of the three activities of the proteasome: chymotrypsin-like activity, trypsin-like activity and caspase-like activity. Cleavage of the fluorophore from the substrate by the proteasome results in fluorescence that can be detected with a fluorescent plate reader.

  8. An Optimal Calibration Method for a MEMS Inertial Measurement Unit

    Directory of Open Access Journals (Sweden)

    Bin Fang

    2014-02-01

    Full Text Available An optimal calibration method for a micro-electro-mechanical inertial measurement unit (MIMU is presented in this paper. The accuracy of the MIMU is highly dependent on calibration to remove the deterministic errors of systematic errors, which also contain random errors. The overlapping Allan variance is applied to characterize the types of random error terms in the measurements. The calibration model includes package misalignment error, sensor-to-sensor misalignment error and bias, and a scale factor is built. The new concept of a calibration method, which includes a calibration scheme and a calibration algorithm, is proposed. The calibration scheme is designed by D-optimal and the calibration algorithm is deduced by a Kalman filter. In addition, the thermal calibration is investigated, as the bias and scale factor varied with temperature. The simulations and real tests verify the effectiveness of the proposed calibration method and show that it is better than the traditional method.

  9. The chemistry behind antioxidant capacity assays.

    Science.gov (United States)

    Huang, Dejian; Ou, Boxin; Prior, Ronald L

    2005-03-23

    This review summarizes the multifaceted aspects of antioxidants and the basic kinetic models of inhibited autoxidation and analyzes the chemical principles of antioxidant capacity assays. Depending upon the reactions involved, these assays can roughly be classified into two types: assays based on hydrogen atom transfer (HAT) reactions and assays based on electron transfer (ET). The majority of HAT-based assays apply a competitive reaction scheme, in which antioxidant and substrate compete for thermally generated peroxyl radicals through the decomposition of azo compounds. These assays include inhibition of induced low-density lipoprotein autoxidation, oxygen radical absorbance capacity (ORAC), total radical trapping antioxidant parameter (TRAP), and crocin bleaching assays. ET-based assays measure the capacity of an antioxidant in the reduction of an oxidant, which changes color when reduced. The degree of color change is correlated with the sample's antioxidant concentrations. ET-based assays include the total phenols assay by Folin-Ciocalteu reagent (FCR), Trolox equivalence antioxidant capacity (TEAC), ferric ion reducing antioxidant power (FRAP), "total antioxidant potential" assay using a Cu(II) complex as an oxidant, and DPPH. In addition, other assays intended to measure a sample's scavenging capacity of biologically relevant oxidants such as singlet oxygen, superoxide anion, peroxynitrite, and hydroxyl radical are also summarized. On the basis of this analysis, it is suggested that the total phenols assay by FCR be used to quantify an antioxidant's reducing capacity and the ORAC assay to quantify peroxyl radical scavenging capacity. To comprehensively study different aspects of antioxidants, validated and specific assays are needed in addition to these two commonly accepted assays. PMID:15769103

  10. e-Calibrations: using the Internet to deliver calibration services in real time at lower cost

    International Nuclear Information System (INIS)

    The National Institute of Standards and Technology (NIST) is expanding into a new frontier in the delivery of measurement services. The Internet will be employed to provide industry with electronic traceability to national standards. This is a radical departure from the traditional modes of traceability and presents many new challenges. The traditional mail-based calibration service relies on sending artifacts to the user, who then mails them back to NIST for evaluation. The new service will deliver calibration results to the industry customer on-demand, in real-time, at a lower cost. The calibration results can be incorporated rapidly into the production process to ensure the highest quality manufacturing. The service would provide the US radiation processing industry with a direct link to the NIST calibration facilities and its expertise, and provide an interactive feedback process between industrial processing and the national measurement standard. Moreover, an Internet calibration system should contribute to the removal of measurement-related trade barriers

  11. Trainee Readiness For Diversity Training

    Directory of Open Access Journals (Sweden)

    Yunhyung Chung

    2013-11-01

    Full Text Available Although trainee readiness is critical for diversity training effectiveness, extant research has not paid attention to the relationship between trainee readiness for diversity training and diversity training outcomes. This study identifies motivational, behavioral, and cognitive trainee readiness for diversity training and proposes a theoretical framework of how individual characteristics (perceived discrimination, demographic attributes, and previous diversity-related experience and organizational characteristics (diversity climate and demographic dissimilarity influence motivational, behavioral, and cognitive trainee readiness for diversity training.

  12. 21 CFR 864.7525 - Heparin assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Heparin assay. 864.7525 Section 864.7525 Food and... HEMATOLOGY AND PATHOLOGY DEVICES Hematology Kits and Packages § 864.7525 Heparin assay. (a) Identification. A heparin assay is a device used to determine the level of the anticoagulant heparin in the...

  13. Multicentre comparison of a diagnostic assay

    DEFF Research Database (Denmark)

    Waters, Patrick; Reindl, Markus; Saiz, Albert;

    2016-01-01

    ) assays in neuromyelitis optica spectrum disorders (NMOSD). METHODS: Coded samples from patients with neuromyelitis optica (NMO) or NMOSD (101) and controls (92) were tested at 15 European diagnostic centres using 21 assays including live (n=3) or fixed cell-based assays (n=10), flow cytometry (n=4...

  14. 21 CFR 864.7490 - Sulfhemoglobin assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Sulfhemoglobin assay. 864.7490 Section 864.7490 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED... assay. (a) Identification. A sulfhemoglobin assay is a device consisting of the reagents,...

  15. 21 CFR 864.7250 - Erythropoietin assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Erythropoietin assay. 864.7250 Section 864.7250... assay. (a) Identification. A erythropoietin assay is a device that measures the concentration of erythropoietin (an enzyme that regulates the production of red blood cells) in serum or urine. This...

  16. 21 CFR 864.7425 - Carboxyhemoglobin assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Carboxyhemoglobin assay. 864.7425 Section 864.7425 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED... assay. (a) Identification. A carboxyhemoglobin assay is a device used to determine the...

  17. 21 CFR 866.3210 - Endotoxin assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Endotoxin assay. 866.3210 Section 866.3210 Food... DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3210 Endotoxin assay. (a) Identification. An endotoxin assay is a device that uses serological techniques in whole blood. The device...

  18. The cryogenic balance design and balance calibration methods

    Science.gov (United States)

    Ewald, B.; Polanski, L.; Graewe, E.

    1992-07-01

    The current status of a program aimed at the development of a cryogenic balance for the European Transonic Wind Tunnel is reviewed. In particular, attention is given to the cryogenic balance design philosophy, mechanical balance design, reliability and accuracy, cryogenic balance calibration concept, and the concept of an automatic calibration machine. It is shown that the use of the automatic calibration machine will improve the accuracy of calibration while reducing the man power and time required for balance calibration.

  19. A Review of Sensor Calibration Monitoring for Calibration Interval Extension in Nuclear Power Plants

    Energy Technology Data Exchange (ETDEWEB)

    Coble, Jamie B.; Meyer, Ryan M.; Ramuhalli, Pradeep; Bond, Leonard J.; Hashemian, Hash; Shumaker, Brent; Cummins, Dara

    2012-08-31

    Currently in the United States, periodic sensor recalibration is required for all safety-related sensors, typically occurring at every refueling outage, and it has emerged as a critical path item for shortening outage duration in some plants. Online monitoring can be employed to identify those sensors that require calibration, allowing for calibration of only those sensors that need it. International application of calibration monitoring, such as at the Sizewell B plant in United Kingdom, has shown that sensors may operate for eight years, or longer, within calibration tolerances. This issue is expected to also be important as the United States looks to the next generation of reactor designs (such as small modular reactors and advanced concepts), given the anticipated longer refueling cycles, proposed advanced sensors, and digital instrumentation and control systems. The U.S. Nuclear Regulatory Commission (NRC) accepted the general concept of online monitoring for sensor calibration monitoring in 2000, but no U.S. plants have been granted the necessary license amendment to apply it. This report presents a state-of-the-art assessment of online calibration monitoring in the nuclear power industry, including sensors, calibration practice, and online monitoring algorithms. This assessment identifies key research needs and gaps that prohibit integration of the NRC-approved online calibration monitoring system in the U.S. nuclear industry. Several needs are identified, including the quantification of uncertainty in online calibration assessment; accurate determination of calibration acceptance criteria and quantification of the effect of acceptance criteria variability on system performance; and assessment of the feasibility of using virtual sensor estimates to replace identified faulty sensors in order to extend operation to the next convenient maintenance opportunity. Understanding the degradation of sensors and the impact of this degradation on signals is key to

  20. Control console of the gamma calibration room

    International Nuclear Information System (INIS)

    The Nuclear Centre of Mexico has a Ionizing Radiation Metrology Center (CMRI). This is in charge of the calibration in Mexico and Latin America of equipment dedicated to radiation measurement as industrial, medical as other fields. The importance to ensure that the equipment stay justly calibrated, it is imposed the necessity of automating the different rooms which the CMRI has. in this case it will be exposed the Calibration room for gamma radiation type. The operation of this application was carried out with the LabVIEW development platform and also in C language. The hardware associated is: personal computer with two cards using the 8255 device, 16 channels with optical isolation to manage input/output TTL type, 16 channels with optical isolation to management of charges to 127 V a.c., and 2 channels for 90V d.c. motors. (Author)

  1. Multichannel radiometer calibration: a new approach

    Science.gov (United States)

    Diaz, Susana; Booth, Charles R.; Armstrong, Roy; Brunat, Claudio; Cabrera, Sergio; Camilion, Carolina; Casiccia, Claudio; Deferrari, Guillermo; Fuenzalida, Humberto; Lovengreen, Charlotte; Paladini, Alejandro; Pedroni, Jorge; Rosales, Alejandro; Zagarese, Horacio; Vernet, Maria

    2005-09-01

    The error in irradiance measured with Sun-calibrated multichannel radiometers may be large when the solar zenith angle (SZA) increases. This could be particularly detrimental in radiometers installed at mid and high latitudes, where SZAs at noon are larger than 50° during part of the year. When a multiregressive methodology, including the total ozone column and SZA, was applied in the calculation of the calibration constant, an important improvement was observed. By combining two different equations, an improvement was obtained at almost all the SZAs in the calibration. An independent test that compared the irradiance of a multichannel instrument and a spectroradiometer installed in Ushuaia, Argentina, was used to confirm the results.

  2. A Calibrating Device for Rogowski Coil Development

    Institute of Scientific and Technical Information of China (English)

    LV Liang; LI Junhao; HUANG Jianjun; JI Shengchang; LI Yanming

    2007-01-01

    A calibrating device for the Rogowski coil is developed,which can be used to calibrate the Rogowski coil having a partial response time within tens of nanoseconds.Its key component is a step current generator,which can generate the output with a rise time of less than 2 ns and a duration of larger than 300 ns.The step current generator is composed by a pulse forming line(PFL)and a pulse transmission line(PTL).A TEM(transverse electromagnetic mode)coaxial measurement unit is used as PTL,and the coil to be calibrated and the referenced standard Rogowski coil can be fixed in the unit.The effect of the dimensions of the TEM unit is discussed theoretically as well as experimentally.

  3. ATLAS Tile calorimeter calibration and monitoring systems

    CERN Document Server

    Chomont, Arthur Rene; The ATLAS collaboration

    2016-01-01

    The ATLAS Tile Calorimeter (TileCal) is the central section of the hadronic calorimeter of the ATLAS experiment and provides important information for reconstruction of hadrons, jets, hadronic decays of tau leptons and missing transverse energy. This sampling calorimeter uses steel plates as absorber and scintillating tiles as active medium. The light produced by the passage of charged particles is transmitted by wavelength shifting fibres to photomultiplier tubes (PMTs), located on the outside of the calorimeter. The readout is segmented into about 5000 cells (longitudinally and transversally), each of them being read out by two PMTs in parallel. To calibrate and monitor the stability and performance of each part of the readout chain during the data taking, a set of calibration systems is used. The TileCal calibration system comprises Cesium radioactive sources, laser and charge injection elements and it allows to monitor and equalize the calorimeter response at each stage of the signal production, from scin...

  4. Oregon State TRIGA reactor power calibration study

    International Nuclear Information System (INIS)

    As a result of a recent review of the Oregon State TRIGA Reactor (OSTR) power calibration procedure, an investigation was performed on the origin and correctness of the OSTR tank factor and the calibration method. It was determined that there was no clear basis for the tank factor which was being used (0.0525 deg. C/kwh) and therefore a new value was calculated (0.0493 deg. C/kwh). The calculational method and likely errors are presented in the paper. In addition, a series of experimental tests were conducted to decide if the power calibration was best performed with or without a mixer, at 100 KW or at 1 MW. The results of these tests along with the final recommendation are presented. (author)

  5. Ion Beam Energy Calibration Method for Accelerator

    International Nuclear Information System (INIS)

    Ion beam energy calibration methods, i e : nuclear reaction method, magnetic field method and calorimeter method were elaborated and studied from its advantage and disadvantage in this paper. Ion beam energy calibration method for accelerator using the method of magnetic field on 3 MV Tandem Accelerator have been carried out at Tiara, JAERI, Japan. The result showed that the energy of ion beam current is 43.56 keV. The result of study conclude that nuclear reaction method generally used to calibrate ion beam energy at the accelerator of energy larger than 2 MeV, calorimetric method for the accelerator electron including linac, magnetic field method for all particle type of accelerator. (author)

  6. Absolute radiometric calibration of the CCRS SAR

    Science.gov (United States)

    Ulander, Lars M. H.; Hawkins, Robert K.; Livingstone, Charles E.; Lukowski, Tom I.

    1991-11-01

    Determining the radar scattering coefficients from SAR (synthetic aperture radar) image data requires absolute radiometric calibration of the SAR system. The authors describe an internal calibration methodology for the airborne Canada Centre for Remote Sensing (CCRS) SAR system, based on radar theory, a detailed model of the radar system, and measurements of system parameters. The methodology is verified by analyzing external calibration data acquired over a 6-month period in 1988 by the C-band radar using HH polarization. The results indicate that the overall error is +/- 0.8 dB (1-sigma) for incidence angles +/- 20 deg from antenna boresight. The dominant error contributions are due to the antenna radome and uncertainties in the elevation angle relative to the antenna boresight.

  7. Kinematic calibration of orthoglide-type mechanisms

    CERN Document Server

    Pashkevich, Anatoly; Wenger, Philippe

    2006-01-01

    The paper proposes a novel calibration approach for the Orthoglide-type mechanisms based on observations of the manipulator leg parallelism during mo-tions between the prespecified test postures. It employs a low-cost measuring system composed of standard comparator indicators attached to the universal magnetic stands. They are sequentially used for measuring the deviation of the relevant leg location while the manipulator moves the TCP along the Cartesian axes. Using the measured differences, the developed algorithm estimates the joint offsets that are treated as the most essential parameters to be adjusted. The sensitivity of the meas-urement methods and the calibration accuracy are also studied. Experimental re-sults are presented that demonstrate validity of the proposed calibration technique

  8. Muon Calibration at SoLid

    CERN Document Server

    Saunders, Daniel

    2016-01-01

    The SoLid experiment aims to make a measurement of very short distance neutrino oscillations using reactor antineutrinos. Key to its sensitivity are the experiments high spatial and energy resolution, combined with a very suitable reactor source and efficient background rejection. The fine segmentation of the detector (cubes of side 5cm), and ability to resolve signals in space and time, gives SoLid the capability to track cosmic muons. In principle a source of background, these turn into a valuable calibration source if they can be cleanly identified. This work presents the first energy calibration results, using cosmic muons, of the 288kg SoLid prototype SM1. This includes the methodology of tracking at SoLid, cosmic ray angular analyses at the reactor site, estimates of the time resolution, and calibrations at the cube level.

  9. Calibration of mixed-polarization interferometric observations

    CERN Document Server

    Marti-Vidal, Ivan; Conway, John; Zensus, Anton J

    2016-01-01

    Heterodyne receivers register the sky signal on either a circular polarization basis (where it is split into left-hand and right-hand circular polarization) or a linear polarization basis (where it is split into horizontal and vertical linear polarization). We study the problem of interferometric observations performed with telescopes that observe on different polarization bases, hence producing visibilities that we call "mixed basis" (i.e., linear in one telescope and circular in the other). We present novel algorithms for the proper calibration and treatment of such interferometric observations and test our algorithms with both simulations and real data. The use of our algorithms will be important for the optimum calibration of forthcoming observations with the Atacama Large mm/submm Array (ALMA) in very-long-baseline interferometry (VLBI) mode. Our algorithms will also allow us to optimally calibrate future VLBI observations at very high data rates (i.e., wide bandwidths), where linear-polarization feeds w...

  10. Development of an immunochromatographic assay based on carbon nanoparticles for the determination of the phytoregulator forchlorfenuron

    NARCIS (Netherlands)

    Suaréz-Pantaleón, C.; Wichers, J.H.; Abad-Somovilla, A.; Amerongen, van A.

    2013-01-01

    Rapid analytical methods enabling the determination of diverse targets are essential in a number of research areas, from clinical diagnostics to feed and food quality and safety. Herein, the development of a quantitative immunochromatographic assay for the detection of the synthetic phytoregulator f

  11. 基于极坐标的摄像机标定%Camera calibration based on polar coordinate

    Institute of Scientific and Technical Information of China (English)

    方旭; 达飞鹏; 郭涛

    2012-01-01

    In order to improve the flexibility of multi-camera calibration and the diversity of calibration boards, the polar coordinate system is used to design the calibration board, and the camera calibration is based on the board. The feature points in the world coordinate are assigned by the designed board, and the positions of the feature points in the image coordinate are identified accordingly. The camera are calibrated after mapping the positions of the feature points in the image coordinate and the world coordinate. The calibration method only requires small sets of correspondences ( about 20 pairs) , and, meanwhile, it can ensure the accuracy and robustness. Small sets of correspondences and the diversity of images can avoid the blocking and inadequate public spaces for multi-camera calibration, which is helpful for the multi-camera calibration. It is more flexible than the traditional multi-camera calibration on the distribution of cameras and boards. Compared with the traditional method with accurate board, the calibration board design and the calibration system are simpler to achieve and they can also ensure the accuracy.%为了改进多摄像机标定实现的方便性和参考标定板的多样性,用极坐标设计标定板并以此来进行摄像机参数的标定.根据设计好的标定板确定世界坐标下的特征点,识别相应的特征点在图像坐标的位置,匹配特征点在图像坐标和世界坐标的位置关系后完成摄像机的标定.该标定方法只需大约20点对左右数据,同时保证标定的结果和鲁棒性;较少的点对和图像的多样性避免了遮挡和公共部分不足的问题,有利于完成多摄像机标定,较传统多摄像机标定方法在分配摄像机和标定板之间的位置关系上更加灵活.与传统的精确标定板的结果比较,在保证精度情况下,标定板设计和整个标定系统的实现则更加简单易行.

  12. Calibration of radiation protection monitoring equipment

    International Nuclear Information System (INIS)

    The facilities and equipment required for calibrating radiation protection monitoring equipment are both complex and expensive. The staff required to operate the equipment and perform the standardization have to be experienced, well trained and technically competent in the use of secondary or primary radiation standards and equipment. Thus an organization cannot commit considerable resources to perform calibration work purely for academic or scientific reasons. In most cases it must be justified by legal requirements pertaining within the country. These legal requirements on measuring devices for radiation protection purposes may be broadly divided into those relating to instruments intended for area monitoring and to those intended for individual monitoring

  13. New volumetric technique for calibrating neutron probes

    International Nuclear Information System (INIS)

    Studies in laboratory conditions were carried out to determine neutron calibration curves. Soils were packer in barrels to attain values of bulk density similar to those in the field. A mariotte reservoir supplies water to the barels in a way that keeps a water table of 10 cm. A water flow is established from this saturated region to the top of the barrel. The water depth is correlated to the numerical integration of the count rate with depth during the process of capillary tise. The coefficient of determination was 0.999. The calibration curve was independent of the initial misture contents of the soil profile. (author)

  14. Time calibration of the ANTARES neutrino telescope

    International Nuclear Information System (INIS)

    The ANTARES deep-sea neutrino telescope comprises a three-dimensional array of photomultipliers to detect the Cherenkov light induced by upgoing relativistic charged particles originating from neutrino interactions in the vicinity of the detector. The large scattering length of light in the deep sea facilitates an angular resolution of a few tenths of a degree for neutrino energies exceeding 10 TeV. In order to achieve this optimal performance, the time calibration procedures should ensure a relative time calibration between the photomultipliers at the level of 1 ns. The methods developed to attain this level of precision are described. (authors)

  15. Intercomparison of Laboratory Radiance Calibration Standards

    Science.gov (United States)

    Pavri, Betina; Chrien, Tom; Green, Robert; Williams, Orlesa

    2000-01-01

    Several standards for radiometric calibration were measured repeatedly with a spectroradiometer in order to understand how they compared in accuracy and stability. The tested radiance standards included a NIST 1000 W bulb and halon panel, two calibrated and stabilized integrating spheres, and a cavity blackbody. Results indicate good agreement between the blackbody and 1000 W bulb/spectralon panel, If these two radiance sources are assumed correct, then the integrating spheres did not conform. to their manufacturer-reported radiances in several regions of the spectrum. More detailed measurements am underway to investigate the discrepancy.

  16. Calibrating Images from the MINERVA Cameras

    Science.gov (United States)

    Mercedes Colón, Ana

    2016-01-01

    The MINiature Exoplanet Radial Velocity Array (MINERVA) consists of an array of robotic telescopes located on Mount Hopkins, Arizona with the purpose of performing transit photometry and spectroscopy to find Earth-like planets around Sun-like stars. In order to make photometric observations, it is necessary to perform calibrations on the CCD cameras of the telescopes to take into account possible instrument error on the data. In this project, we developed a pipeline that takes optical images, calibrates them using sky flats, darks, and biases to generate a transit light curve.

  17. Local Volatility Calibration Using An Adjoint Proxy

    Directory of Open Access Journals (Sweden)

    Gabriel TURINICI

    2008-11-01

    Full Text Available We document the calibration of the local volatility in a framework similar to Coleman, Li and Verma. The quality of a surface is assessed through a functional to be optimized; the specificity of the approach is to separate the optimization (performed with any suitable optimization algorithm from the computation of the functional where we use an adjoint (as in L. Jiang et. al. to obtain an approximation; moreover our main calibration variable is the implied volatility (the procedure can also accommodate the Greeks. The procedure performs well on benchmarks from the literature and on FOREX data.

  18. Calibration of the solar neutrino detectors

    Energy Technology Data Exchange (ETDEWEB)

    Caccianiga, Barbara; Re, Alessandra Carlotta [Universita degli Studi Milano (Italy); INFN, Milano (Italy)

    2016-04-15

    Calibrations have been crucial for the success of solar neutrino experiments. In this contribution we review the calibration strategies adopted by different solar neutrino experiments. In particular, we will emphasize their common critical aspects and their main differences. In order to do so, we will schematically divide the solar neutrino experiments in two groups: those based on radiochemical techniques, i.e. Homestake, Gallex/GNO, SAGE and those based on real-time techniques i.e. Kamiokande, Super-Kamiokande, SNO, Borexino and KamLAND. (orig.)

  19. E-book Management with Calibre Software

    OpenAIRE

    N P, Jamsheer

    2013-01-01

    Keeping pace with the technology is a big challenge for modern libraries. Reading habits and requirements of the library users are changing. Libraries have to provide access to the varying forms of information available. In addition to the conventional book lending, users should be given access to e-books. This artcle examines how to manage e-books in a library using calibre e-book management software. Calibre is a highly user friendly personal e-book manager. This free software can be used i...

  20. Electrical Calibration for Calvet Type Microcalorimetry

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The deyjce construction and working principle of Calvet type heat conductive microcalorimetry are generally ineroduced in this paper. Based on previous work, we design a reactor vessel and its electrical calibration equipment according to our special study systems. The heat constant of our improved microalorimetry is gotten by means of electrical calibration on the basis of Tian's equation. The results gotten by in tegration method are identical with ones gotten by peak method. It shows that our improved device works well enough to develop further studies.``

  1. On Calibration of pH Meters

    OpenAIRE

    Da-Ming Zhu; Cheng, K. L.

    2005-01-01

    The calibration of pH meters including the pH glass electrode, ISE electrodes, buffers, and the general background for calibration are reviewed. Understanding of basic concepts of pH, pOH, and electrode mechanism is emphasized. New concepts of pH, pOH, as well as critical examination of activity, and activity coefficients are given. The emergence of new solid state pH electrodes and replacement of the salt bridge with a conducting wire have opened up a new horizon for pH measurements. A pH bu...

  2. SI-Traceable Calibrations of Celestial Objects

    Science.gov (United States)

    Cramer, C. E.; Lykke, K. R.; Woodward, J. T.

    2016-05-01

    Photometric calibration is currently the leading source of systematic uncertainty in supernova surveys that aim to determine the nature of dark energy. The bulk of this uncertainty is due to imperfect knowledge of the spectral energy distribution of stars used as primary standards. We review the challenges associated with establishing an absolute calibration of stellar spectra and describe how it is possible to do better by using recent advances in optical metrology, paying particular attention to the measurement chain establishing SI-traceability and reporting of measurement uncertainties.

  3. Making transuranic assay measurements using modern controllers

    International Nuclear Information System (INIS)

    This paper describes methodology and computer-controlled instrumentation developed at the Los Alamos National Laboratory that accurately performs nondestructive assays of large containers bearing transuranic wastes and nonradioactive matrix materials. These assay systems can measure fissile isotopes with 1-mg sensitivity and spontaneous neutron-emitting isotopes at a 10-mg sensitivity. The assays are performed by neutron interrogation, detection, and counting in a custom assay chamber. An International Business Machines Personal Computer (IBM-PC) is used to control the CAMAC-based instrumentation system that acquires the assay data. 6 refs., 7 figs

  4. Establishment of Immunoradiometric Assay for Carcinoembryonic Antigen

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Two anti-CEA monoclonal antibodies are used, one is coated on the microtiter plate, the other is labeled to make 125I-CEAMcAb. The one-step assay is established based on immunoradiometric assay(IRMA). The sensitivity of the assay is 0.5 μ g/L. The intra-assay CVs and the inter-assay CVs are lower than 10.0% and 15.0%, respectively. The analytical recoveries are ranged from 97.4% to 107.8%. The reference cut-out value of 35 normal serum is lower than

  5. CERN Diversity Newsletter - July 2015

    CERN Document Server

    Kaltenhauser, Kristin; CERN. Geneva. HR Department

    2015-01-01

    The first official edition of the CERN Diversity Newsletter, informing on recent and ongoing diversity activities, and interesting reads, videos and other links related to diversity. Subscribe here: https://diversity.web.cern.ch/2015/07/subscribe-diversity-newsletter

  6. Predictive Assay For Cancer Targets

    Energy Technology Data Exchange (ETDEWEB)

    Suess, A; Nguyen, C; Sorensen, K; Montgomery, J; Souza, B; Kulp, K; Dugan, L; Christian, A

    2005-09-19

    Early detection of cancer is a key element in successful treatment of the disease. Understanding the particular type of cancer involved, its origins and probable course, is also important. PhIP (2-amino-1-methyl-6 phenylimidazo [4,5-b]pyridine), a heterocyclic amine produced during the cooking of meat at elevated temperatures, has been shown to induce mammary cancer in female, Sprague-Dawley rats. Tumors induced by PhIP have been shown to contain discreet cytogenetic signature patterns of gains and losses using comparative genomic hybridization (CGH). To determine if a protein signature exists for these tumors, we are analyzing expression levels of the protein products of the above-mentioned tumors in combination with a new bulk protein subtractive assay. This assay produces a panel of antibodies against proteins that are either on or off in the tumor. Hybridization of the antibody panel onto a 2-D gel of tumor or control protein will allow for identification of a distinct protein signature in the tumor. Analysis of several gene databases has identified a number of rat homologs of human cancer genes located in these regions of gain and loss. These genes include the oncogenes c-MYK, ERBB2/NEU, THRA and tumor suppressor genes EGR1 and HDAC3. The listed genes have been shown to be estrogen-responsive, suggesting a possible link between delivery of bio-activated PhIP to the cell nucleus via estrogen receptors and gene-specific PhIP-induced DNA damage, leading to cell transformation. All three tumors showed similar silver staining patterns compared to each other, while they all were different than the control tissue. Subsequent screening of these genes against those from tumors know to be caused by other agents may produce a protein signature unique to PhIP, which can be used as a diagnostic to augment optical and radiation-based detection schemes.

  7. Urban thermal diversity

    Institute of Scientific and Technical Information of China (English)

    KoenSTEEMERS; MarylisRAMOS; MariaSINOU

    2003-01-01

    This paper introduces the interrelationships between urban form, microclimate and thermal comfort. It draws on recent research of monitoring, surveying and modelling urban thermal characteristics and proposes a method of mapping urban diversity. Because the urban context provides a rich and varied environment that influences the way we use urban spaces (movement, sequence, activity) and how we feel in them (stimulation, thermal comfort), the aim here is to highlight the notion of diversity. Thus thermal diversity is used as a measure of the urban environment, rather than more conventional spatially or temporally fixed average values.

  8. Religious diversity and pluralism

    DEFF Research Database (Denmark)

    Ahlin, Lars; Borup, Jørn; Fibiger, Marianne Qvortrup;

    2012-01-01

    . Religious diversity has grown in Denmark with the arrival of new immigrant groups and with new forms and interpretations of traditional religious and spiritual traditions. More importantly, the relations and interactions between religious groups -- the hallmarks of religious pluralism -- are still incipient....... Both religious diversity and religious pluralism build on assumptions of stable relationships between religion and religious adherents and clear-cut boundaries between religious groups, assumptions which may be difficult to sustain in late modern societies. This article gives an overview of the Project......'s findings and discusses theoretical challenges related to religious diversity and religious pluralism....

  9. Calibration Procedures on Oblique Camera Setups

    Science.gov (United States)

    Kemper, G.; Melykuti, B.; Yu, C.

    2016-06-01

    Beside the creation of virtual animated 3D City models, analysis for homeland security and city planning, the accurately determination of geometric features out of oblique imagery is an important task today. Due to the huge number of single images the reduction of control points force to make use of direct referencing devices. This causes a precise camera-calibration and additional adjustment procedures. This paper aims to show the workflow of the various calibration steps and will present examples of the calibration flight with the final 3D City model. In difference to most other software, the oblique cameras are used not as co-registered sensors in relation to the nadir one, all camera images enter the AT process as single pre-oriented data. This enables a better post calibration in order to detect variations in the single camera calibration and other mechanical effects. The shown sensor (Oblique Imager) is based o 5 Phase One cameras were the nadir one has 80 MPIX equipped with a 50 mm lens while the oblique ones capture images with 50 MPix using 80 mm lenses. The cameras are mounted robust inside a housing to protect this against physical and thermal deformations. The sensor head hosts also an IMU which is connected to a POS AV GNSS Receiver. The sensor is stabilized by a gyro-mount which creates floating Antenna -IMU lever arms. They had to be registered together with the Raw GNSS-IMU Data. The camera calibration procedure was performed based on a special calibration flight with 351 shoots of all 5 cameras and registered the GPS/IMU data. This specific mission was designed in two different altitudes with additional cross lines on each flying heights. The five images from each exposure positions have no overlaps but in the block there are many overlaps resulting in up to 200 measurements per points. On each photo there were in average 110 well distributed measured points which is a satisfying number for the camera calibration. In a first step with the help of

  10. CALIBRATION PROCEDURES ON OBLIQUE CAMERA SETUPS

    Directory of Open Access Journals (Sweden)

    G. Kemper

    2016-06-01

    Full Text Available Beside the creation of virtual animated 3D City models, analysis for homeland security and city planning, the accurately determination of geometric features out of oblique imagery is an important task today. Due to the huge number of single images the reduction of control points force to make use of direct referencing devices. This causes a precise camera-calibration and additional adjustment procedures. This paper aims to show the workflow of the various calibration steps and will present examples of the calibration flight with the final 3D City model. In difference to most other software, the oblique cameras are used not as co-registered sensors in relation to the nadir one, all camera images enter the AT process as single pre-oriented data. This enables a better post calibration in order to detect variations in the single camera calibration and other mechanical effects. The shown sensor (Oblique Imager is based o 5 Phase One cameras were the nadir one has 80 MPIX equipped with a 50 mm lens while the oblique ones capture images with 50 MPix using 80 mm lenses. The cameras are mounted robust inside a housing to protect this against physical and thermal deformations. The sensor head hosts also an IMU which is connected to a POS AV GNSS Receiver. The sensor is stabilized by a gyro-mount which creates floating Antenna –IMU lever arms. They had to be registered together with the Raw GNSS-IMU Data. The camera calibration procedure was performed based on a special calibration flight with 351 shoots of all 5 cameras and registered the GPS/IMU data. This specific mission was designed in two different altitudes with additional cross lines on each flying heights. The five images from each exposure positions have no overlaps but in the block there are many overlaps resulting in up to 200 measurements per points. On each photo there were in average 110 well distributed measured points which is a satisfying number for the camera calibration. In a first

  11. Summary of KOMPSAT-5 Calibration and Validation

    Science.gov (United States)

    Yang, D.; Jeong, H.; Lee, S.; Kim, B.

    2013-12-01

    Korean Multi-Purpose Satellite 5 (KOMPSAT-5), equipped with high resolution X-band (9.66 GHz) Synthetic Aperture Radar (SAR), is planning to be launched on August 22, 2013. With the satellite's primary mission objective being providing Geographical Information System (GIS), Ocean monitoring and Land management, and Disaster and ENvironment monitoring (GOLDEN), it is expected that its applications for scientific research on geographical processes will be extensive. In order to meet its mission objective, the KOMPSAT-5 will provide three different kinds of SAR imaging modes; High Resolution Mode (1 m resolution, 5 km swath), Standard Mode (3 m resolution, 30 km swath), and Wide Swath Mode (20 m resolution, 100 km swath). The KOMPSAT-5 will be operated in a 550 km sun-synchronous, dawn- dusk orbit with a 28-day ground repeat cycle providing valuable image information on Earth surface day-or-night and even in bad weather condition. After successful launch of the satellite, it will go through Launch and Early Operation (LEOP) and In-Orbit Testing (IOT) period about for 6 months to carry out various tests on satellite bus and payload systems. The satellite bus system will be tested during the first 3 weeks after the launch focusing on the Attitude and Orbit Control Subsystem (AOCS) and Integrated GPS Occultation Receiver (IGOR) calibration. With the completion of bus system test, the SAR payload system will be calibrated during initial In-Flight check period (11 weeks) by the joint effort of Thales Alenia Space Italy (TAS-I) and Korea Aerospace Research Institute (KARI). The pointing and relative calibration will be carried out during this period by analyzing the doppler frequency and antenna beam pattern of reflected microwave signal from selected regions with uniform backscattering coefficients (e.g. Amazon rainforest). A dedicated SAR calibration, called primary calibration, will be allocated at the end of LEOP for 12 weeks to perform thorough calibration activities

  12. X ray spectra and qualities for use in diagnostic radiology and equipment calibration

    International Nuclear Information System (INIS)

    The goal of this work was the standardization of radiation qualities of diagnostic X ray equipment of the Assay Laboratory of the Institute for Radiation Protection and Dosimetry (IRD) of the National Commission of Nuclear Energy, Brazil. X ray spectra were determined from pulse height distribution measured directly on the primary beam using a high pure planar Ge detector. A program was developed to convert pulse height distribution into radiation spectra in the range from 20 to 150 keV. X ray qualities based on those used by the 'Physikalish-Technish Bundesantalt' (PTB) primary laboratory were implanted in three radiological equipment of the Assay Laboratory. These qualities simulate radiation beams on patients submitted to typical radiological examinations. Besides the spectrometric system, a reference measurement system based on an ionization chamber calibrated in air kerma was used to establish parameters such as kilovoltage, first and second half-value layer, mean energy, effective energy and inherent filtration. Our data have shown that the implantation of these radiation qualities in the Assay Laboratory results on a metrological basis for calibration of dose measurement assemblies and kV-meters, like those used by IRD to evaluate the parameters of X ray equipment around the country. A catalogue of spectral data resulting from this work is a data bank that allows various applications like dose calculation using Monte Carlo simulation techniques. (author)

  13. Activity assay of membrane transport proteins

    Institute of Scientific and Technical Information of China (English)

    Hao Xie

    2008-01-01

    Membrane transport proteins are integral membrane proteins and considered as potential drug targets. Activity assay of transport proteins is essential for developing drugs to target these proteins. Major issues related to activity assessment of transport proteins include availability of transporters,transport activity of transporters, and interactions between ligands and transporters. Researchers need to consider the physiological status of proteins (bound in lipid membranes or purified), availability and specificity of substrates, and the purpose of the activity assay (screening, identifying, or comparing substrates and inhibitors) before choosing appropriate assay strategies and techniques. Transport proteins bound in vesicular membranes can be assayed for transporting substrate across membranes by means of uptake assay or entrance counterflow assay. Alternatively, transport proteins can be assayed for interactions with ligands by using techniques such as isothermal titration calorimetry, nuclear magnetic resonance spectroscopy, or surface plasmon resonance. Other methods and techniques such as fluorometry, scintillation proximity assay, electrophysiological assay, or stopped-flow assay could also be used for activity assay of transport proteins. In this paper the major strategies and techniques for activity assessment of membrane transport proteins are reviewed.

  14. Total Measurement Uncertainty for the Plutonium Finishing Plant (PFP) Segmented Gamma Scan Assay System

    CERN Document Server

    Fazzari, D M

    2001-01-01

    This report presents the results of an evaluation of the Total Measurement Uncertainty (TMU) for the Canberra manufactured Segmented Gamma Scanner Assay System (SGSAS) as employed at the Hanford Plutonium Finishing Plant (PFP). In this document, TMU embodies the combined uncertainties due to all of the individual random and systematic sources of measurement uncertainty. It includes uncertainties arising from corrections and factors applied to the analysis of transuranic waste to compensate for inhomogeneities and interferences from the waste matrix and radioactive components. These include uncertainty components for any assumptions contained in the calibration of the system or computation of the data. Uncertainties are propagated at 1 sigma. The final total measurement uncertainty value is reported at the 95% confidence level. The SGSAS is a gamma assay system that is used to assay plutonium and uranium waste. The SGSAS system can be used in a stand-alone mode to perform the NDA characterization of a containe...

  15. Calibration Issues and Operating System Requirements for Electron-Probe Microanalysis

    Science.gov (United States)

    Carpenter, P.

    2006-01-01

    Instrument purchase requirements and dialogue with manufacturers have established hardware parameters for alignment, stability, and reproducibility, which have helped improve the precision and accuracy of electron microprobe analysis (EPMA). The development of correction algorithms and the accurate solution to quantitative analysis problems requires the minimization of systematic errors and relies on internally consistent data sets. Improved hardware and computer systems have resulted in better automation of vacuum systems, stage and wavelength-dispersive spectrometer (WDS) mechanisms, and x-ray detector systems which have improved instrument stability and precision. Improved software now allows extended automated runs involving diverse setups and better integrates digital imaging and quantitative analysis. However, instrumental performance is not regularly maintained, as WDS are aligned and calibrated during installation but few laboratories appear to check and maintain this calibration. In particular, detector deadtime (DT) data is typically assumed rather than measured, due primarily to the difficulty and inconvenience of the measurement process. This is a source of fundamental systematic error in many microprobe laboratories and is unknown to the analyst, as the magnitude of DT correction is not listed in output by microprobe operating systems. The analyst must remain vigilant to deviations in instrumental alignment and calibration, and microprobe system software must conveniently verify the necessary parameters. Microanalysis of mission critical materials requires an ongoing demonstration of instrumental calibration. Possible approaches to improvements in instrument calibration, quality control, and accuracy will be discussed. Development of a set of core requirements based on discussions with users, researchers, and manufacturers can yield documents that improve and unify the methods by which instruments can be calibrated. These results can be used to

  16. Comparison of Droplet Digital PCR and Quantitative PCR Assays for Quantitative Detection of Xanthomonas citri Subsp. citri.

    Science.gov (United States)

    Zhao, Yun; Xia, Qingyan; Yin, Youping; Wang, Zhongkang

    2016-01-01

    Droplet digital polymerase chain reaction (ddPCR) is a novel molecular biology technique providing absolute quantification of target nucleic acids without the need for an external calibrator. Despite its emerging applications in medical diagnosis, there are few reports of its use for the detection of plant pathogens. This work was designed to assess the diagnosis potential of the ddPCR for absolute quantitative detection of Xanthomonas citri subsp. citri, a quarantine plant pathogenic bacterium that causes citrus bacterial canker in susceptible Citrus species. We transferred an established quantitative PCR (qPCR) assay for citrus bacterial canker diagnosis directly to the ddPCR format and compared the performance of the two methods. The qPCR assay has a broader dynamic range compared to the ddPCR assay and the ddPCR assay has a significantly higher degree of sensitivity compared to the qPCR assay. The influence of PCR inhibitors can be reduced considerably in the ddPCR assay because the collection of end-point fluorescent signals and the counting of binomial events (positive or negative droplets) are associated with a Poisson algorithm. The ddPCR assay also shows lower coefficient of variation compared to the qPCR assay especially in low target concentration. The linear association of the measurements by ddPCR and qPCR assays is strong (Pearson correlation = 0.8633; Pvalue of ddPCR technology in the diagnosis of plant disease and quarantine applications. PMID:27427975

  17. Calibration of Local Area Weather Radar—Identifying significant factors affecting the calibration

    Science.gov (United States)

    Pedersen, Lisbeth; Jensen, Niels Einar; Madsen, Henrik

    2010-07-01

    A Local Area Weather Radar (LAWR) is an X-band weather radar developed to meet the needs of high resolution rainfall data for hydrological applications. The LAWR system and data processing methods are reviewed in the first part of this paper, while the second part of the paper focuses on calibration. The data processing for handling the partial beam filling issue was found to be essential to the calibration. LAWR uses a different calibration process compared to conventional weather radars, which use a power-law relationship between reflectivity and rainfall rate. Instead LAWR uses a linear relationship of reflectivity and rainfall rate as result of the log transformation carried out by the logarithmic receiver as opposed to the linear receiver of conventional weather radars. Based on rain gauge data for a five month period from a dense network of nine gauges within a 500 × 500 m area and data from a nearby LAWR, the existing calibration method was tested and two new methods were developed. The three calibration methods were verified with three external gauges placed in different locations. It can be concluded that the LAWR calibration uncertainties can be reduced by 50% in two out of three cases when the calibration is based on a factorized 3 parameter linear model instead of a single parameter linear model.

  18. Phytochemical diversity drives plant–insect community diversity

    OpenAIRE

    Richards, Lora A.; Dyer, Lee A.; Forister, Matthew L.; Smilanich, Angela M.; Dodson, Craig D.; Leonard, Michael D.; Jeffrey, Christopher S.

    2015-01-01

    Phytochemical diversity is a key component of functional diversity. Challenges in quantifying phytochemical diversity have limited our understanding of the causes and consequences of variation in phytochemical diversity across plant species and families. Here we show that phytochemical diversity across dozens of plant species predicts herbivore diversity, herbivore specialization, phototoxicity, herbivory, and attack by natural enemies. Our approach and findings provide a framework for future...

  19. A Rapid and Efficient Luminescence-based Method for Assaying Phosphoglycosyltransferase Enzymes.

    Science.gov (United States)

    Das, Debasis; Walvoort, Marthe T C; Lukose, Vinita; Imperiali, Barbara

    2016-01-01

    Phosphoglycosyltransferases (PGTs) are families of integral membrane proteins with intriguingly diverse architectures. These enzymes function to initiate many important biosynthetic pathways including those leading to peptidoglycan, N-linked glycoproteins and lipopolysaccharide O-antigen. In spite of tremendous efforts, characterization of these enzymes remains a challenge not only due to the inherent difficulties associated with the purification of integral membrane proteins but also due to the limited availability of convenient assays. Current PGT assays include radioactivity-based methods, which rely on liquid-liquid or solid-liquid extractions, multienzyme systems linked to lactate dehydrogenase and NAD(+) generation, and HPLC-based approaches, all of which may suffer from low sensitivity and low throughput. Herein, we present the validation of a new luminescence-based assay (UMP-Glo) for measuring activities of PGT enzymes. This assay measures UMP, the by-product of PGT reactions, in a sensitive and quantitative manner by measuring the luminescence output in a discontinuous coupled assay system. The assay is rapid and robust in nature, and also compatible with microtiter plate formats. Activity and kinetic parameters of PglC, a PGT from Campylobacter jejuni, were quickly established using this assay. The efficacy of the assay was further corroborated using two different PGTs; PglC from Helicobacter pullorum and WecA from Thermatoga maritima. PMID:27624811

  20. SCIAMACHY Level 1 data: calibration concept and in-flight calibration

    Directory of Open Access Journals (Sweden)

    G. Lichtenberg

    2006-01-01

    Full Text Available The calibration of SCIAMACHY was thoroughly checked since the instrument was launched on-board ENVISAT in February 2002. While SCIAMACHY's functional performance is excellent since launch, a number of technical difficulties have appeared, that required adjustments to the calibration. The problems can be separated into three types: (1 Those caused by the instrument and/or platform environment. Among these are the high water content in the satellite structure and/or MLI layer. This results in the deposition of ice on the detectors in channels 7 and 8 which seriously affects the retrievals in the IR, mostly because of the continuous change of the slit function caused by scattering of the light through the ice layer. Additionally a light leak in channel 7 severely hampers any retrieval from this channel. (2 Problems due to errors in the on-ground calibration and/or data processing affecting for example the radiometric calibration. A new approach based on a mixture of on-ground and in-flight data is shortly described here. (3 Problems caused by principal limitations of the calibration concept, e.g. the possible appearance of spectral structures after the polarisation correction due to unavoidable errors in the determination of atmospheric polarisation. In this paper we give a complete overview of the calibration and problems that still have to be solved. We will also give an indication of the effect of calibration problems on retrievals where possible. Since the operational processing chain is currently being updated and no newly processed data are available at this point in time, for some calibration issues only a rough estimate of the effect on Level 2 products can be given. However, it is the intention of this paper to serve as a future reference for detailed studies into specific calibration issues.

  1. Spectrophotometric validation of assay method for selected medicinal plant extracts

    Directory of Open Access Journals (Sweden)

    Matthew Arhewoh

    2014-09-01

    Full Text Available Objective: To develop UV spectrophotometric assay validation methods for some selected medicinal plant extracts.Methods: Dried, powdered leaves of Annona muricata (AM and Andrographis paniculata (AP as well as seeds of Garcinia kola (GK and Hunteria umbellata (HU were separately subjected to maceration using distilled water. Different concentrations of the extracts were scanned spectrophotometrically to obtain wavelengths of maximum absorbance. The different extracts were then subjected to validation studies following international guidelines at the respective wavelengths obtained.Results: The results showed linearity at peak wavelengths of maximum absorbance of 292, 280, 274 and 230 nm for GK, HU, AM and AP, respectively. The calibration curves for the different concentrations of the extract gave R2 values ranging from 0.9831 for AM to 0.9996 for AP the inter-day and intra-day precision study showed that the relative standard deviation (% was ≤ 10% for all the extracts.Conclusion: The aqueous extracts and isolates of these plants can be assayed and monitored using these wavelengths.

  2. Measuring dabigatran with the dilute Russell viper venom confirm assay in an anticoagulation clinic population.

    Science.gov (United States)

    McGlasson, David L; Fritsma, George A

    2016-01-01

    The dabigatran dose-response is predictable; however, it is necessary to measure plasma levels in a variety of clinical conditions. We evaluated a novel dabigatran measure - the 'dilute Russell viper venom confirm (DRVVC) assay' - against current developmental assays and a reference method. We measured plasma dabigatran and compared results from the Stago Sta-Clot DRVVC assay, Stago Ecarin Chromogenic Assay, Biophen Hemoclot Thrombin Inhibitor, and liquid chromatography tandem mass spectrometry. We obtained dabigatran calibrators and controls from Biophen, and performed the coagulation assays using a Stago STA-R Evolution coagulometer. Liquid chromatography tandem mass spectrometry method specimens were performed on an AB Sciex instrument at LabCorp. We enrolled 97 anticoagulation clinic patients (mean age 76 years) who were taking 150 mg dabigatran twice daily. All had creatinine clearances above 30 ml/min; patients were not excluded for concurrent medications or health issues. Citrated blood specimens were processed immediately, and stored at -70°C. We did not correlate collection time with medication time. We employed descriptive statistics, analysis of variance, and the Bland-Altman difference plot to assess the data. The range for all assays was 11.6-917 ng/ml. Analysis of variance generated a P value of 0.1 and Bland-Altman differences were all below 4.0% compared with DRVVC. The DRVVC measures dabigatran with validity comparable to other methods.

  3. Managing Protean Diversity

    DEFF Research Database (Denmark)

    Marfelt, Mikkel Mouritz; Muhr, Sara Louise

    2016-01-01

    Recently, global workforce diversity and its management have received criticism for not paying attention to the contextual influence stemming from socially constructed dialectics of power and politics. These contextual dynamics, however, tend to be viewed as external to the organization. In this ......Recently, global workforce diversity and its management have received criticism for not paying attention to the contextual influence stemming from socially constructed dialectics of power and politics. These contextual dynamics, however, tend to be viewed as external to the organization...... management, the project dissolves through micropolitics and power dynamics. We contribute to the critical literature on workforce diversity by identifying how organizational contextual dynamics influence the way the concept of workforce diversity is constructed and understood at work. Based on these findings...

  4. Managing biological diversity

    Science.gov (United States)

    Samson, Fred B.; Knopf, Fritz L.

    1993-01-01

    Biological diversity is the variety of life and accompanying ecological processes (Off. Technol. Assess. 1987, Wilcove and Samson 1987, Keystone 1991). Conservation of biological diversity is a major environmental issue (Wilson 1988, Counc. Environ. Quality 1991). The health and future of the earth's ecological systems (Lubchenco et al. 1991), global climate change (Botkin 1990), and an ever-increasing rate in loss of species, communities, and ecological systems (Myers 1990) are among issues drawing biological diversity to the mainstream of conservation worldwide (Int. Union Conserv. Nat. and Nat. Resour. [IUCN] et al. 1991). The legal mandate for conserving biological diversity is now in place (Carlson 1988, Doremus 1991). More than 19 federal laws govern the use of biological resources in the United States (Rein 1991). The proposed National Biological Diversity Conservation and Environmental Research Act (H.R. 585 and S.58) notes the need for a national biological diversity policy, would create a national center for biological diversity research, and recommends a federal interagency strategy for ecosystem conservation. There are, however, hard choices ahead for the conservation of biological diversity, and biologists are grappling with how to set priorities in research and management (Roberts 1988). We sense disillusion among field biologists and managers relative to how to operationally approach the seemingly overwhelming charge of conserving biological diversity. Biologists also need to respond to critics like Hunt (1991) who suggest a tree farm has more biological diversity than an equal area of old-growth forest. At present, science has played only a minor role in the conservation of biological diversity (Weston 1992) with no unified approach available to evaluate strategies and programs that address the quality and quantity of biological diversity (Murphy 1990, Erwin 1992). Although actions to conserve biological diversity need to be clearly defined by

  5. Diversity and Generation X.

    Science.gov (United States)

    Davis, S K

    2001-09-01

    Managing Generation X (1965-1980) to be of better service to patients and organizations is a challenge for nurse managers. This article provides action scenarios that assist in understanding diversity and generations.

  6. In-Flight Pitot-Static Calibration

    Science.gov (United States)

    Foster, John V. (Inventor); Cunningham, Kevin (Inventor)

    2016-01-01

    A GPS-based pitot-static calibration system uses global output-error optimization. High data rate measurements of static and total pressure, ambient air conditions, and GPS-based ground speed measurements are used to compute pitot-static pressure errors over a range of airspeed. System identification methods rapidly compute optimal pressure error models with defined confidence intervals.

  7. Computerized Techniques for Calibrating Pressure Balances

    Science.gov (United States)

    Simpson, D. I.

    1994-01-01

    Pressure balances are generally calibrated by the cross-floating technique, where the forces acting on two similar devices in hydrostatic equilibrium are compared. It is a skilled and time-consuming process which has not previously lent itself to significant automation; computers have mostly been used only to calculate results after measurements have been taken. The objective of the present work was to develop real-time computerized measurement techniques to ease the calibration task, which would fully integrate into a single package with versatile software for calculating and displaying results. The calibration process is now conducted by studying graphical computer displays which derive their inputs from differential-pressure transducers and capacitance or optical displacement sensors. The mass imbalance between oil-operated pressure balances is calculated by interpolating between changes in piston rate-of-fall. Differential-pressure transducers are used to estimate mass imbalances between gas-operated balances, and a quick in situ method for determining their sensitivity has been developed. The new techniques have been successfully applied to a variety of pressure balance designs and substantial reductions in calibration times have been achieved. Reduced levels of scatter have revealed small systematic differences between gauge and absolute modes of operation.

  8. Calibration of the MACHO photometry database

    Energy Technology Data Exchange (ETDEWEB)

    Alcock, A

    1998-10-23

    The MACHO Project is a microlensing survey that monitors the brightnesses of ~60 million stars in the Large Magellanic Cloud (LMC), Small Magellanic Cloud, and Galactic bulge. The database presently contains more photometric measurements than previously recorded in the history of astronomy. We describe the calibration of the MACHO two-color photometry and transformation to the standard Kron-Cousins V and R system. This allows for proper comparison with all other observations on the Kron-Cousins standard system. The highest precision calibrations are for ~9 million stars in the LMC bar. For these stars, independent photometric measurements in field-overlap regions indicate standard deviations δvR = 0.020 mag. Calibrated MACHO photometry data are compared with published photometric sequences and with new Hubble Space Telescope observations. We additionally describe the first application of these calibrated data: the construction of the "efficiency" color-magnitude diagram which will be used to calculate our experimental sensitivity for detecting microlensing in the LMC.

  9. Calibration of the ALLEGRO resonant detector

    Energy Technology Data Exchange (ETDEWEB)

    McHugh, Martin P [Department of Physics, Loyola University, New Orleans, Louisiana 70118 (United States); Johnson, Warren W [Department of Physics and Astronomy, Louisiana State University, Baton Rouge, Louisiana 70803 (United States); Hamilton, William O [Department of Physics and Astronomy, Louisiana State University, Baton Rouge, Louisiana 70803 (United States); Hanson, Jonathan [Department of Physics and Astronomy, Louisiana State University, Baton Rouge, Louisiana 70803 (United States); Heng, Ik Siong [University of Glasgow, Glasgow G12 8QQ (United Kingdom); McNeese, Daniel [Department of Physics, Loyola University, New Orleans, Louisiana 70118 (United States); Miller, Phillip [Department of Physics and Astronomy, Louisiana State University, Baton Rouge, Louisiana 70803 (United States); Nettles, Damon [Department of Physics and Astronomy, Louisiana State University, Baton Rouge, Louisiana 70803 (United States); Weaver, Jordan [Department of Physics and Astronomy, Louisiana State University, Baton Rouge, Louisiana 70803 (United States); Zhang Ping [Department of Physics and Astronomy, Louisiana State University, Baton Rouge, Louisiana 70803 (United States)

    2005-09-21

    We describe a method for calibrating the ALLEGRO resonant detector. The resulting response function can be used to transform the observed data backwards to gravitational strain data. These data are the input to a cross-correlation analysis to search for stochastic gravitational waves.

  10. Soil moisture calibration of TDR multilevel probes

    Directory of Open Access Journals (Sweden)

    Serrarens Daniel

    2000-01-01

    Full Text Available Time domain reflectometry (TDR probes are increasingly used for field estimation of soil water content. The objective of this study was to evaluate the accuracy of the multilevel TDR probe under field conditions. For this purpose, eight such TDR probes were installed in small plots that were seeded with beans and sorghum. Data collection from the probes was such that soil moisture readings were automated and logged using a standalone field unit. Neutron probe measurements were used to calibrate the TDR probes. Soil-probe contact and soil compaction were critical to the accuracy of the TDR, especially when a number of TDR probes are combined for a single calibration curve. If each probe is calibrated individually, approximate measurement errors were between 0.005 and 0.015 m³ m-3. However, measurement errors doubled to approximately 0.025 to 0.03 m³ m-3, when TDR probes were combined to yield a single calibration curve.

  11. Ground calibrations of Nuclear Compton Telescope

    Science.gov (United States)

    Chiu, Jeng-Lun; Liu, Zhong-Kai; Bandstra, Mark S.; Bellm, Eric C.; Liang, Jau-Shian; Perez-Becker, Daniel; Zoglauer, Andreas; Boggs, Steven E.; Chang, Hsiang-Kuang; Chang, Yuan-Hann; Huang, Minghuey A.; Amman, Mark; Chiang, Shiuan-Juang; Hung, Wei-Che; Lin, Chih-Hsun; Luke, Paul N.; Run, Ray-Shine; Wunderer, Cornelia B.

    2010-07-01

    The Nuclear Compton Telescope (NCT) is a balloon-borne soft gamma ray (0.2-10 MeV) telescope designed to study astrophysical sources of nuclear line emission and polarization. The heart of NCT is an array of 12 cross-strip germanium detectors, designed to provide 3D positions for each photon interaction with full 3D position resolution to imaging, effectively reduces background, and enables the measurement of polarization. The keys to Compton imaging with NCT's detectors are determining the energy deposited in the detector at each strip and tracking the gamma-ray photon interaction within the detector. The 3D positions are provided by the orthogonal X and Y strips, and by determining the interaction depth using the charge collection time difference (CTD) between the anode and cathode. Calibrations of the energy as well as the 3D position of interactions have been completed, and extensive calibration campaigns for the whole system were also conducted using radioactive sources prior to our flights from Ft. Sumner, New Mexico, USA in Spring 2009, and from Alice Springs, Australia in Spring 2010. Here we will present the techniques and results of our ground calibrations so far, and then compare the calibration results of the effective area throughout NCT's field of view with Monte Carlo simulations using a detailed mass model.

  12. Analysis and calibration techniques for superconducting resonators

    Science.gov (United States)

    Cataldo, Giuseppe; Wollack, Edward J.; Barrentine, Emily M.; Brown, Ari D.; Moseley, S. Harvey; U-Yen, Kongpop

    2015-01-01

    A method is proposed and experimentally explored for in-situ calibration of complex transmission data for superconducting microwave resonators. This cryogenic calibration method accounts for the instrumental transmission response between the vector network analyzer reference plane and the device calibration plane. Once calibrated, the observed resonator response is analyzed in detail by two approaches. The first, a phenomenological model based on physically realizable rational functions, enables the extraction of multiple resonance frequencies and widths for coupled resonators without explicit specification of the circuit network. In the second, an ABCD-matrix representation for the distributed transmission line circuit is used to model the observed response from the characteristic impedance and propagation constant. When used in conjunction with electromagnetic simulations, the kinetic inductance fraction can be determined with this method with an accuracy of 2%. Datasets for superconducting microstrip and coplanar-waveguide resonator devices were investigated and a recovery within 1% of the observed complex transmission amplitude was achieved with both analysis approaches. The experimental configuration used in microwave characterization of the devices and self-consistent constraints for the electromagnetic constitutive relations for parameter extraction are also presented.

  13. Planck 2015 results. V. LFI calibration

    CERN Document Server

    Ade, P A R; Ashdown, M; Aumont, J; Baccigalupi, C; Banday, A J; Barreiro, R B; Bartolo, N; Battaglia, P; Battaner, E; Benabed, K; Benoît, A; Benoit-Lévy, A; Bernard, J -P; Bersanelli, M; Bielewicz, P; Bock, J J; Bonaldi, A; Bonavera, L; Bond, J R; Borrill, J; Bouchet, F R; Bucher, M; Burigana, C; Butler, R C; Calabrese, E; Cardoso, J -F; Catalano, A; Chamballu, A; Christensen, P R; Colombi, S; Colombo, L P L; Crill, B P; Curto, A; Cuttaia, F; Danese, L; Davies, R D; Davis, R J; de Bernardis, P; de Rosa, A; de Zotti, G; Delabrouille, J; Dickinson, C; Diego, J M; Dole, H; Donzelli, S; Doré, O; Douspis, M; Ducout, A; Dupac, X; Efstathiou, G; Elsner, F; Enßlin, T A; Eriksen, H K; Fergusson, J; Finelli, F; Forni, O; Frailis, M; Franceschi, E; Frejsel, A; Galeotta, S; Galli, S; Ganga, K; Giard, M; Giraud-Héraud, Y; Gjerløw, E; González-Nuevo, J; Górski, K M; Gratton, S; Gregorio, A; Gruppuso, A; Hansen, F K; Hanson, D; Harrison, D L; Henrot-Versillé, S; Herranz, D; Hildebrandt, S R; Hivon, E; Hobson, M; Holmes, W A; Hornstrup, A; Hovest, W; Huffenberger, K M; Hurier, G; Jaffe, A H; Jaffe, T R; Juvela, M; Keihänen, E; Keskitalo, R; Kisner, T S; Knoche, J; Krachmalnicoff, N; Kunz, M; Kurki-Suonio, H; Lagache, G; Lähteenmäki, A; Lamarre, J -M; Lasenby, A; Lattanzi, M; Lawrence, C R; Leahy, J P; Leonardi, R; Lesgourgues, J; Levrier, F; Liguori, M; Lilje, P B; Linden-Vørnle, M; López-Caniego, M; Lubin, P M; Macías-Pérez, J F; Maggio, G; Maino, D; Mandolesi, N; Mangilli, A; Maris, M; Martin, P G; Martínez-González, E; Masi, S; Matarrese, S; McGehee, P; Meinhold, P R; Melchiorri, A; Mendes, L; Mennella, A; Migliaccio, M; Mitra, S; Montier, L; Morgante, G; Mortlock, D; Moss, A; Munshi, D; Murphy, J A; Naselsky, P; Nati, F; Natoli, P; Netterfield, C B; Nørgaard-Nielsen, H U; Novikov, D; Novikov, I; Paci, F; Pagano, L; Pajot, F; Paoletti, D; Partridge, B; Pasian, F; Patanchon, G; Pearson, T J; Peel, M; Perdereau, O; Perotto, L; Perrotta, F; Pettorino, V; Piacentini, F; Pierpaoli, E; Pietrobon, D; Pointecouteau, E; Polenta, G; Pratt, G W; Prézeau, G; Prunet, S; Puget, J -L; Rachen, J P; Rebolo, R; Reinecke, M; Remazeilles, M; Renzi, A; Rocha, G; Romelli, E; Rosset, C; Rossetti, M; Roudier, G; Rubiño-Martín, J A; Rusholme, B; Sandri, M; Santos, D; Savelainen, M; Scott, D; Seiffert, M D; Shellard, E P S; Spencer, L D; Stolyarov, V; Sutton, D; Suur-Uski, A -S; Sygnet, J -F; Tauber, J A; Tavagnacco, D; Terenzi, L; Toffolatti, L; Tomasi, M; Tristram, M; Tucci, M; Tuovinen, J; Türler, M; Umana, G; Valenziano, L; Valiviita, J; Van Tent, B; Vassallo, T; Vielva, P; Villa, F; Wade, L A; Wandelt, B D; Watson, R; Wehus, I K; Wilkinson, A; Yvon, D; Zacchei, A; Zonca, A

    2015-01-01

    We present a description of the pipeline used to calibrate the Planck Low Frequency Instrument (LFI) timelines into thermodynamic temperatures for the Planck 2015 data release, covering 4 years of uninterrupted operations. As in the 2013 data release, our calibrator is provided by the spin-synchronous modulation of the CMB dipole, exploiting both the orbital and solar components. Our 2015 LFI analysis provides an independent Solar dipole estimate in excellent agreement with that of HFI and within $1\\sigma$ (0.3 % in amplitude) of the WMAP value. This 0.3 % shift in the peak-to-peak dipole temperature from WMAP and a global overhaul of the iterative calibration code increases the overall level of the LFI maps by 0.45 % (30 GHz), 0.64 % (44 GHz), and 0.82 % (70 GHz) in temperature with respect to the 2013 Planck data release, thus reducing the discrepancy with the power spectrum measured by WMAP. We estimate that the LFI calibration uncertainty is at the level of 0.20 % for the 70 GHz map, 0.26 % for the 44 GHz...

  14. The External Calibrator for Hydrogen Observatories

    CERN Document Server

    Jacobs, Daniel C; Bowman, Judd; Neben, Abraham R; Stinnett, Benjamin; Turner, Lauren

    2016-01-01

    Multiple instruments are pursuing constraints on dark energy, observing reionization and opening a window on the dark ages through the detection and characterization of the 21cm hydrogen line across the redshift spectrum, from nearby to z=25. These instruments, including CHIME in the sub-meter and HERA in the meter bands, are wide-field arrays with multiple-degree beams, typically operating in transit mode. Accurate knowledge of their primary beams is critical for separation of bright foregrounds from the desired cosmological signals, but difficult to achieve through astronomical observations alone. Previous beam calibration work has focused on model verification and does not address the need of 21cm experiments for routine beam mapping, to the horizon, of the as-built array. We describe the design and methodology of a drone-mounted calibrator, the External Calibrator for Hydrogen Observatories (ECHO), that aims to address this need. We report on a first set of trials to calibrate low-frequency dipoles and co...

  15. Beam Calibration of Radio Telescopes with Drones

    Science.gov (United States)

    Chang, Chihway; Monstein, Christian; Refregier, Alexandre; Amara, Adam; Glauser, Adrian; Casura, Sarah

    2015-11-01

    We present a multifrequency far-field beam map for the 5-m dish telescope at the Bleien Observatory measured using a commercially available drone. We describe the hexacopter drone used in this experiment, the design of the flight pattern, and the data analysis scheme. This is the first application of this calibration method to a single-dish radio telescope in the far-field. The high signal-to-noise ratio data allows us to characterize the beam pattern with high accuracy out to at least the fourth side-lobe. The resulting two-dimensional beam pattern is compared with that derived from a more traditional calibration approach using an astronomical calibration source. We discuss the advantages of this method compared to other beam calibration methods. Our results show that this drone-based technique is very promising for ongoing and future radio experiments, where the knowledge of the beam pattern is key to obtaining high-accuracy cosmological and astronomical measurements.

  16. Beam calibration of radio telescopes with drones

    CERN Document Server

    Chang, Chihway; Refregier, Alexandre; Amara, Adam; Glauser, Adrian; Casura, Sarah

    2015-01-01

    We present a multi-frequency far-field beam map for the 5m dish telescope at the Bleien Observatory measured using a commercially available drone. We describe the hexacopter drone used in this experiment, the design of the flight pattern, and the data analysis scheme. This is the first application of this calibration method to a single dish radio telescope in the far-field. The high signal-to-noise data allows us to characterise the beam pattern with high accuracy out to at least the 4th side-lobe. The resulting 2D beam pattern is compared with that derived from a more traditional calibration approach using an astronomical calibration source. We discuss the advantages of this method compared to other beam calibration methods. Our results show that this drone-based technique is very promising for ongoing and future radio experiments, where the knowledge of the beam pattern is key to obtaining high-accuracy cosmological and astronomical measurements.

  17. Space Power Facility Reverberation Chamber Calibration Report

    Science.gov (United States)

    Lewis, Catherine C.; Dolesh, Robert J.; Garrett, Michael J.

    2014-01-01

    This document describes the process and results of calibrating the Space Environmental Test EMI Test facility at NASA Plum Brook Space Power Facility according to the specifications of IEC61000-4-21 for susceptibility testing from 100 MHz to 40 GHz. The chamber passed the field uniformity test, in both the empty and loaded conditions, making it the world's largest Reverberation Chamber.

  18. CALIBRATION OF A WEARABLE GLUCOSE SENSOR

    NARCIS (Netherlands)

    SCHMIDT, FJ; AALDERS, AL; SCHOONEN, AJM; DOORENBOS, H

    1992-01-01

    Calibration of glucose sensors proved difficult for electrodes with immobilized glucose-oxidase. The correlation between the sensitivity of the electrodes in vitro and in vivo appeared to be poor. We developed a new type of glucose sensor, based on a microdialysis system, in which an oxygen electrod

  19. Calibration of sound velocimeter in pure water

    Science.gov (United States)

    Li, Zhiwei; Zhang, Baofeng; Li, Tao; Zhu, Junchao; Xie, Ziming

    2016-01-01

    Accurate measurement of sound speed is important to calibrate a sound velocity profiler which provides real-time sound velocity to the sonar equipment in oceanographic survey. The sound velocity profiler calculates the sound speed by measuring the time-of-flight of a 1 MHz single acoustic pulse to travel over about 300 mm path. A standard sound velocimeter instrument was invited to calibrate the sound velocity profiler in pure water at temperatures of 278,283, 288, 293, 298, 303 and 308K in a thermostatic vessel at one atmosphere. The sound velocity profiler was deployed in the thermostatic vessel alongside the standard sound velocimeter instrument and two platinum resistance thermometers (PRT) which were calibrated to 0.002k by comparison with a standard PRT. Time of flight circuit board was used to measure the time-of-flight to 22 picosecond precision. The sound speed which was measured by the sound velocity profiler was compared to the standard sound speed calculated by UNESCO to give the laboratory calibration coefficients and was demonstrated agreement with CTD-derived sound speed using Del Grosso's seawater equation after removing a bias.

  20. Tanks for liquids: calibration and errors assessment

    International Nuclear Information System (INIS)

    After a brief reference to some of the problems raised by tanks calibration, two methods, theoretical and experimental are presented, so as to achieve it taking into account measurement errors. The method is applied to the transfer of liquid from one tank to another. Further, a practical example is developed. (author)