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Sample records for calendula officinalis lin

  1. Biochemical structure of Calendula officinalis.

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    Korakhashvili, A; Kacharava, T; Kiknavelidze, N

    2007-01-01

    Calendula officinalis is a well known medicinal herb. It is common knowledge that its medicinal properties are conditioned on biologically active complex substances of Carotin (Provitamin A), Stearin, Triterpiniod, Plavonoid, Kumarin, macro and micro compound elements. Because of constant need in raw material of Calendula officinalis, features of its ontogenetic development agro-biological qualities in various eco regions of Georgia were investigated. The data of biologically active compounds, biochemical structure and the maintenance both in flowers and in others parts of plant is presented; the pharmacological activity and importance in medicine was reviewed.

  2. Seed germination of Calendula officinalis 'Carola' in response to temperature

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    Calendula (Calendula officinalis 'Carola') is a potential agronomic oilseed crop with application in the paint, coating, and cosmetic industry. Calendula has historically been used for herbal medicinal purposes and an ornamental plant. With the discovery that calendula seeds contain high concentrati...

  3. “IN VITRO” MULTIPLICATION OF CALENDULA OFFICINALIS L.

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    Vantu Smaranda

    2015-11-01

    Full Text Available The aim of the present study was to develop a regeneration procedures for Calendula officinalis L., as analternative for biomass production. Calendula officinalis L. (Asteraceae is an important medicinal plant species withmultitherapeutic, cosmetic, values. Meristematic explants taken from seedlings of Calendula officinalis L. germinated inaseptic conditions were tested for their regenerative potential. The regeneration of whole plants was obtained in twosteps: the shoots were excised and transferred to fresh medium and then rooting of these shoots was achieved on the samemedium with 0,02 mg/l benzylaminopurine and 1 mg/l 2,4 dichlorophenoxyacetic acid. The excised shoots weresubcultured for roots induction. Regenerated plants were transferred to ex vitro conditions for an acclimatisation period

  4. Growth and yield response of calendula (Calendula officinalis) to sowing date in the northern U.S.

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    Calendula (Calendula officinalis L.) seed is a rich source of the conjugated C18:3 fatty acid calendic acid and can serve as a replacement for VOCs in many industrial chemicals such as paints, coatings and adhesives. Calendula is widely adapted to temperate climates and may be a beneficial rotationa...

  5. Amelioration of myocardial ischemic reperfusion injury with Calendula officinalis.

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    Ray, Diptarka; Mukherjee, Subhendu; Falchi, Mario; Bertelli, Aldo; Das, Dipak K

    2010-12-01

    Calendula officinalis of family Asteraceae, also known as marigold, has been widely used from time immemorial in Indian and Arabic cultures as an anti-inflammatory agent to treat minor skin wound and infections, burns, bee stings, sunburn and cancer. At a relatively high dose, calendula can lower blood pressure and cholesterol. Since inflammatory responses are behind many cardiac diseases, we sought to evaluate if calendula could be cardioprotective against ischemic heart disease Two groups of hearts were used: the treated rat hearts were perfused with calendula solution at 50 mM in KHB buffer (in mM: sodium chloride 118, potassium chloride 4.7, calcium chloride 1.7, sodium bicarbonate 25, potassium biphosphate 0.36, magnesium sulfate 1.2, and glucose 10) for 15 min prior to subjecting the heart to ischemia, while the control group was perfused with the buffer only. Calendula achieved cardioprotection by stimulating left ventricular developed pressure and aortic flow as well as by reducing myocardial infarct size and cardiomyocyte apoptosis. Cardioprotection appears to be achieved by changing ischemia reperfusion-mediated death signal into a survival signal by modulating antioxidant and anti-inflammatory pathways as evidenced by the activation of Akt and Bcl2 and depression of TNFα. The results further strengthen the concept of using natural products in degeneration diseases like ischemic heart disease.

  6. Effect of Calendula officinalis cream on achilles tendon healing.

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    Aro, A A; Perez, M O; Vieira, C P; Esquisatto, M A M; Rodrigues, R A F; Gomes, L; Pimentel, E R

    2015-02-01

    In recent years, the scientific community has undertaken research on plant extracts, searching for compounds with pharmacological activities that can be used in diverse fields of medicine. Calendula officinalis L. is known to have antioxidant, anti-inflammatory, antibacterial, and wound healing properties when used to treat skin burns. Therefore, the purpose of this study was to analyze the effects of C. officinalis on the initial phase of Achilles tendon healing. Wistar rats were separated in three groups: Calendula (Cal)-rats with a transected tendon were treated with topical applications of C. officinalis cream and then euthanized 7 days after injury; Control (C)-rats were treated with only vehicle after transection; and Normal (N)-rats without tenotomy. Higher concentrations of hydroxyproline (an indicator of total collagen) and non-collagenous proteins were observed in the Cal group in relation to the C group. Zymography showed no difference in the amount of the isoforms of metalloproteinase-2 and of metalloproteinase-9, between C and Cal groups. Polarization microscopy images analysis showed that the Cal group presented a slightly higher birefringence compared with the C group. In sections of tendons stained with toluidine blue, the transected groups presented higher metachromasy as compared with the N group. Immunocytochemistry analysis for chondroitin-6-sulfate showed no difference between the C and Cal groups. In conclusion, the topical application of C. officinalis after tendon transection increases the concentrations of collagen and non-collagenous proteins, as well as the collagen organization in the initial phase of healing.

  7. Solid lipid nanoparticles for delivery of Calendula officinalis extract.

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    Arana, Lide; Salado, Clarisa; Vega, Sandra; Aizpurua-Olaizola, Oier; de la Arada, Igor; Suarez, Tatiana; Usobiaga, Aresatz; Arrondo, José Luis R; Alonso, Alicia; Goñi, Félix M; Alkorta, Itziar

    2015-11-01

    Solid lipid nanoparticles (SLN) composed of long-chain fatty acids (palmitic acid, stearic acid or arachidic acid), Epikuron 200 (purified phosphatidylcholine), and bile salts (cholate, taurocholate or taurodeoxycholate) have been prepared by dilution of a microemulsion. A total of five different systems were prepared, and characterized by photon correlation spectroscopy, transmission electron microscopy, differential scanning calorimetry, and infrared spectroscopy. The SLN formulation showing optimal properties (lowest size and polydispersity index and highest zeta potential) was obtained with stearic acid and taurodeoxycholate as cosurfactant. This formulation was loaded with Calendula officinalis extract, a natural compound used on ophthalmic formulations given its anti-inflammatory, emollient, and wound repairing activity. Calendula-loaded SLN preparations were characterized in order to determine loading capacity and entrapment efficiency. In vitro cytotoxicity and wound healing efficacy of Calendula-loaded SLN compared to that of a free plant extract were evaluated on a conjunctival epithelium cell line WKD. Our results suggest that this SLN formulation is a safe and solvent-free Calendula extract delivery system which could provide a controlled therapeutic alternative for reducing disease-related symptoms and improving epithelium repair in ocular surface.

  8. Calendula officinalis: Central depressive effect and subacute toxicity

    OpenAIRE

    2009-01-01

    Flores da Calendula officinalis L. (calêndula) vêm sendo utilizadas popularmente como cicatrizante, antiinflamatório e sedativo, entre outras atividades. Os principais componentes químicos encontrados nas flores são óleos essenciais, ácido salicílico, carotenóides, flavonóides, taninos e saponinas triterpênicas. Atividades ansiolítica e analgésica foram relatadas em plantas que apresentam flavonóides em sua composição. Nesse trabalho a atividade do extrato etanólico das flores da ...

  9. Wound healing activity of flower extract of Calendula officinalis.

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    Preethi, Korengath C; Kuttan, Ramadasan

    2009-01-01

    The effects of oral and topical application of Calendula officinalis flower extract on excision wounds made in rats were checked. The parameters assessed were the days needed for re-epithelization and percentage of wound closure. The hydroxy proline and hexosamine content in the granuloma tissue of the wound was also measured. The percentage of wound closure was 90.0% in the extract-treated group, whereas the control group showed only 51.1% on the eighth day of wounding (p < .01). The days needed for re-epithelization were 17.7 for the control animals; extract treatment at a dose of 20 or 100 mg/kg b.wt reduced the period to 14 and 13 days, respectively. A significant increase was observed in the hydroxy proline and hexosamine content in the extract-treated group compared with the untreated animals. The data indicate potent wound healing activity ofC. officinalis extract.

  10. Studies on spasmogenic and spasmolytic activities of Calendula officinalis flowers.

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    Bashir, Samra; Janbaz, Khalid H; Jabeen, Qaiser; Gilani, Anwar H

    2006-10-01

    The aqueous-ethanol extract of Calendula officinalis flowers (Co.Cr) was studied for its possible spasmolytic and spasmogenic effects in isolated gut preparations. In rabbit jejunum, Co.Cr caused a dose-dependent (0.03-3.0 mg/mL) relaxation of spontaneous and K+-induced contractions, suggestive of calcium channel blockade (CCB). In a few preparations, a mild non-reproducible spasmogenic effect was observed at lower doses, followed by relaxation. The CCB effect was confirmed when pretreatment of the jejunum preparations with Co.Cr produced a dose-dependent rightward shift in the Ca(++) dose-response curves, similar to that of verapamil. Activity-directed fractionation revealed that the spasmolytic activity of the plant was concentrated in its organic fractions. The aqueous fraction exhibited a marked atropine sensitive spasmogenic effect but was found to be devoid of any spasmolytic effect. These data indicate that the crude extract of Calendula officinalis flowers contains both spasmolytic and spasmogenic constituents, exhibiting these effects through calcium channel blocking and cholinergic activities and this study provides a scientific base for its traditional use in abdominal cramps and constipation.

  11. Analysis of carotenoid composition in petals of calendula (Calendula officinalis L.).

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    Kishimoto, Sanae; Maoka, Takashi; Sumitomo, Katsuhiko; Ohmiya, Akemi

    2005-11-01

    Nineteen carotenoids were identified in extracts of petals of orange- and yellow-flowered cultivars of calendula (Calendula officinalis L.). Ten carotenoids were unique to orange-flowered cultivars. The UV-vis absorption maxima of these ten carotenoids were at longer wavelengths than that of flavoxanthin, the main carotenoid of calendula petals, and it is clear that these carotenoids are responsible for the orange color of the petals. Six carotenoids had a cis structure at C-5 (C-5'), and it is conceivable that these (5Z)-carotenoids are enzymatically isomerized at C-5 in a pathway that diverges from the main carotenoid biosynthesis pathway. Among them, (5Z,9Z)-lycopene (1), (5Z,9Z,5'Z,9'Z)-lycopene (3), (5'Z)-gamma-carotene (4), and (5'Z,9'Z)-rubixanthin (5) has never before been identified. Additionally, (5Z,9Z,5'Z)-lycopene (2) has been reported only as a synthesized compound.

  12. Assessment of In vitro Sun Protection Factor of Calendula Officinalis L. (Asteraceae) Essential Oil Formulation.

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    Mishra, Ak; Mishra, A; Chattopadhyay, P

    2012-01-01

    The present study was undertaken to study the sunscreen activity of herbal formulation. There is no evidence of the sun protection factor (SPF) studies on essential oil of Calendula flowers (Calendula officinalis L., Asteraceae). The study investigates the in vitro SPF by ultraviolet specrtophotometry method of Calendula flower oil in a cream formulation. Calendula oil was isolated by Clavenger's apparatus, compositions were identified by GC-MS and the cream of calendula flower oil was prepared by homogenization method followed by evaluation for physical parameters. The sun protection factor of cream was evaluated by in vitro method employing UV-visible spectrophotometer (Shimazdu-1600). The SPF of Calendula oil in cream formulation exhibited good activity (SPF = 14.84 ± 0.16). Finding of this study suggested that calendula oil cream can be used to protect the skin from UV radiations in form of sunscreen cream and to maintain the natural pigmentation of the skin.

  13. Toxicological studies on hydroalcohol extract of Calendula officinalis L.

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    Silva, Erick J R; Gonçalves, Eduardo S; Aguiar, Fábio; Evêncio, Liriane B; Lyra, Mariana M A; Coelho, Maria Cristina O C; Fraga, Maria do Carmo C A; Wanderley, Almir G

    2007-04-01

    The hydroalcohol extract (HAE) of Calendula officinalis L. was evaluated for its acute toxicity by the oral route in rats and mice and for the subacute effect on hematological, biochemical and morphologic parameters in rats. In the acute toxicity test, HAE failed to cause death in the animals after administration of oral doses up to 5.0 g/kg. Oral treatment with HAE at 0.025, 0.25, 0.5 and 1.0 g/kg did not induce hematological alterations when compared with the control group. In the biochemical parameters, there was an increase in blood urea nitrogen (BUN) and in alanine transaminase (ALT) levels. Morphological examination of the brain, kidney and heart did not show any alteration. However, inflammatory sites were found in the lung and liver, which were associated, respectively, with oral gavage and a possible hepatotoxic effect. HAE was non-toxic in rats, although there was evidence of renal and liver overload.

  14. DETERMINATION OF ANTIMICROBIAL ACTIVITY OF EXTRACTS OF CALENDULA OFFICINALIS FLOWERS

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    P. V. Afanasyeva

    2016-01-01

    Full Text Available Pot marigold (Calendula officinalis L. is one of the most popular medicinal plants in the Russian Federation and abroad. The wide range of pharmacological activity of this medicinal plant is determined by carotenoids, flavonoids, saponins. These biologically active substances give total therapeutic effect of flowers of Calendula officinalis and medicines on base of pot marigold. This paper discusses the results of comparative investigations for a determination of antimicrobial activity of aqueous and aqueous- alcoholic extracts from pot marigold flowers. Detection of the minimum inhibitory concentration (MIC was carried out by using the method of double serial dilutions in broth. The following microorganisms were used as test cultures: Bacillus cereus, Candida albicans, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The study showed that the widest spectrum of antibacterial activity has water extract of pot marigold flowers. As for Pseudomonas aeruginosa the most active medicine is tincture (1:10 with 70% alcohol. As for Escherichia coli the only phytopharmaceutical – water extract of marigold flowers, reveals antimicrobial activity. Against Bacillus cereus the most effective properties was indicated for tincture (1:5 with 70% ethanol and the liquid extract (1:2 with 70% alcohol. In case of Candida albicans, tincture (1:10 with 70% alcohol exhibited the highest activity.

  15. Evaluation of chemical enhancement on phytoremediation effect of Cd-contaminated soils with Calendula officinalis L.

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    Liu, Jianv; Zhou, Qixing; Wang, Song

    2010-07-01

    The popular ornamental plant Calendula officinalis L was studied for its potential application in the phytoremediation of cadmium (Cd)-contaminated soils. Enhancements to the Cd accumulation by the application of sodium dodecyl sulfate (SDS), ethylenediaminetriacetic acid (EDTA) and ethylenegluatarotriacetic acid (EGTA) to the soil were investigated. Under these chemically enhanced treatments, EDTA was observed to be toxic to the plants leading to retarded growth. However, the application of SDS and/or EGTA was shown to result in significantly increased plant biomass (p Calendula officinalis L. for applications of phytoremediation of Cd-contaminated sites.

  16. Biological Activities of Oleanolic Acid Derivatives from Calendula officinalis Seeds.

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    Zaki, Ahmed; Ashour, Ahmed; Mira, Amira; Kishikawa, Asuka; Nakagawa, Toshinori; Zhu, Qinchang; Shimizu, Kuniyoshi

    2016-05-01

    Phytochemical examination of butanol fraction of Calendula officinalis seeds led to the isolation of two compounds identified as 28-O-β-D-glucopyranosyl-oleanolic acid 3-O-β-D-glucopyranosyl (1→3)-β-D-glucopyranosiduronic acid (CS1) and oleanolic acid 3-O-β-D-glucopyranosyl (1→3)-β-D-glucopyranosiduronic acid (CS2). Biological evaluation was carried out for these two compounds such as melanin biosynthesis inhibitory, hyaluronic acid production activities, anti obesity using lipase inhibition and adipocyte differentiation as well as evaluation of the protective effect against hydrogen peroxide induced neurotoxicity in neuro-2A cells. The results showed that, compound CS2 has a melanin biosynthesis stimulatory activity; however, compound CS1 has a potent stimulatory effect for the production of hyaluronic acid on normal human dermal fibroblast from adult (NHDF-Ad). Both compounds did not show any inhibitory effect on both lipase and adipocyte differentiation. Compound CS2 could protect neuro-2A cells and increased cell viability against H2 O2 . These activities (melanin biosynthesis stimulatory and protective effect against H2 O2 of CS2 and hyaluronic acid productive activities of these triterpene derivatives) have been reported for the first time. Copyright © 2016 John Wiley & Sons, Ltd.

  17. Mechanized harvesting and primary processing of Calendula officinalis L. inflorescences

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    Branislav Veselinov

    2014-04-01

    Full Text Available Manual harvesting of marigold (Calendula officinalis L. inflorescences results in superior quality, but requires enormous manpower. The objectives of the research were to examine the possibility of mechanized harvesting of marigold inflorescences by virtual rotating comb-type chamomile harvester, widely used in South Eastern Europe, and to develop the mechanical separator for the inflorescences from the harvested mass. The impact of coefficient R, ratio of the harvester’s penetration into the inflorescences horizon and the width of inflorescences band (i.e. the average value of the highest and lowest inflorescences span on the harvest yield, was tested first. Separation was performed by a bespoke separator that uses five cascades of oscillating sieves made of longitudinally situated rods. Influence of oscillation frequencies and rod distances on separation efficiency was investigated. It was found that, for coefficient R value of 1.3, an average of 97% of the total inflorescences yield can be harvested. The proportion of inflorescences with stem length up to 2 cm was 65% and 35% for stems over 2 cm. The result of mechanized harvest was the reduction in both the number of succeeding harvests and the yield, due to bud damages and elimination. The highest separation efficiency was obtained by using 2.7 Hz oscillation frequency and combination of 6/8 mm rods’ distances in sieves. After three passes of the harvested mass through the device, approximately 92% of inflorescences were separated. Future investigations should be directed towards solving the issue of mechanical shortening of the inflorescences stems and evaluating the economic viability of mechanized harvesting within the proposed procedure.

  18. Biological Activities of Asteraceae (Achillea millefolium and Calendula officinalis) and Lamiaceae (Melissa officinalis and Origanum majorana) Plant Extracts.

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    García-Risco, Mónica R; Mouhid, Lamia; Salas-Pérez, Lilia; López-Padilla, Alexis; Santoyo, Susana; Jaime, Laura; Ramírez de Molina, Ana; Reglero, Guillermo; Fornari, Tiziana

    2017-03-01

    Asteraceae (Achillea millefolium and Calendula officinalis) and Lamiaceae (Melissa officinalis and Origanum majorana) extracts were obtained by applying two sequential extraction processes: supercritical fluid extraction with carbon dioxide, followed by ultrasonic assisted extraction using green solvents (ethanol and ethanol:water 50:50). The extracts were analyzed in terms of the total content of phenolic compounds and the content of flavonoids; the volatile oil composition of supercritical extracts was analyzed by gas chromatography and the antioxidant capacity and cell toxicity was determined. Lamiaceae plant extracts presented higher content of phenolics (and flavonoids) than Asteraceae extracts. Regardless of the species studied, the supercritical extracts presented the lowest antioxidant activity and the ethanol:water extracts offered the largest, following the order Origanum majorana > Melissa officinalis ≈ Achillea millefolium > Calendula officinalis. However, concerning the effect on cell toxicity, Asteraceae (especially Achillea millefolium) supercritical extracts were significantly more efficient despite being the less active as an antioxidant agent. These results indicate that the effect on cell viability is not related to the antioxidant activity of the extracts.

  19. Efeito cicatrizante e atividade antibacteriana da Calendula officinalis L. cultivada no Brasil Healing effect and antibacterial activity of Calendula officinalis L. cultivated in Brazil

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    L.M.L. Parente

    2009-01-01

    Full Text Available Desde a antiguidade propriedades medicinais são atribuídas às flores da Calendula officinalis L. (Asteraceae destacando-se a atividade cicatrizante. Estudos sobre a atividade geral de plantas medicinais na cicatrização vêm sendo realizados, sem especificar sobre qual das fases da cicatrização a planta atua. Neste trabalho a atividade cicatrizante e antiinflamatória do extrato etanólico das flores da C. officinalis cultivada no Brasil foi avaliada em feridas cutâneas de ratos Wistar, por meio de avaliação macroscópica e histológica. A atividade antimicrobiana do extrato e das frações hexânica e diclorometano também foi avaliada. A atividade antiinflamatória do extrato etanólico da calêndula foi atribuída à diminuição da exsudação serosa, da hiperemia, da deposição de fibrina e da hiperplasia epidermal, além de resultar em crostas mais delgadas e umedecidas. Observaram-se também aumento de colágeno no tecido de granulação e efeito antibacteriano. Assim, o extrato etanólico da calêndula atuou de forma positiva sobre a atividade cicatricial em feridas cutâneas de ratos, bem como apresentou atividade antibacteriana in vitro.Since ancient history medicinal properties are attributed to flowers of Calendula officinalis L. (Asteraceae, mainly concerning its healing activity. Studies about the general activity of medicinal plants in healing wounds have been conducted without specifying in which healing phase the plant acts. In this work, the anti-inflammatory and healing activity of the ethanolic extract of C. officinalis flowers cultured in Brazil was evaluated in cutaneous wounds of Wistar rats through macroscopic and histological evaluation. The antibacterial activity of the extract and dichloromethane and hexane fractions was also evaluated. The anti-inflammatory activity of calendula ethanolic extract led to a reduction in plasmatic exudation, hyperemia, fibrin deposition and epidermal hyperplasia, besides

  20. Bioecological features and corrective properties of Achillea filipendulina Lam., Calendula officinalis L. and Hypericum perforatum L.

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    Elshan Shamilov

    2013-04-01

    Full Text Available The study is devoted to investigations on bioecological features and corrective properties of two species from the family Asteraceae Dumort. (Achillea filipendulina Lam. and Calendula officinalisL. and one species from the family Hypericaceae Juss. (Hypericum perforatumL. which are growing in the flora of Azerbaijan. It has revealed that the extract obtained from the collection of these plants shows corrective influence.

  1. Comparing the effects of Calendula officinalis and clotrimazole on vaginal Candidiasis: A randomized controlled trial.

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    Saffari, Elnaz; Mohammad-Alizadeh-Charandabi, Sakineh; Adibpour, Mohammad; Mirghafourvand, Mojgan; Javadzadeh, Yousef

    2016-11-23

    This triple-blind trial examined the effects of Calendula officinalis vaginal cream on the treatment of vaginal Candidiasis (primary outcome) and sexual function (secondary outcome). Married women aged 18-45 years with vaginal Candidiasis (n = 150) were recruited from April to October 2014 and randomized into Calendula and clotrimazole groups, using 5-g vaginal cream every night for seven nights. Clinical and laboratory assessments were conducted at 10-15 and 30-35 days after intervention and the female sexual function index was assessed at 30-35 days. Six women were lost to follow-up. The frequency of testing negative for Candidiasis in the Calendula group was significantly lower at the first (49% vs. 74%; odds ratio (OR) 0.32; 95% confidence interval (CI) 0.16-0.67) but higher at the second (77% vs. 34%; OR 3.1; 95% CI 1.5-6.2) follow-up compared to the clotrimazole group. The frequency of most signs and symptoms were almost equal in the two groups at the first follow-up, but were significantly lower in the Calendula group at the second follow-up. Sexual function had almost equal significant improvement in both groups. Calendula vaginal cream appears to have been effective in the treatment of vaginal Candidiasis and to have a delayed but greater long-term effect compared to clotrimazole.

  2. The evaluation of trifloxystrobin in protection of Calendula officinalis (Pot marigold) against Erysiphe cichoracearum DC.

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    Kurzawińska, H; Duda-Surman, J

    2006-01-01

    The aim of the two-years field's examinations was the evaluation of the fungicide Zato 50 WG (biologically active substances BAS--trifloxystrobin 50%) in protection of Calendula officinalis (Pot marigold) against Erysiphe cichoracearum. Mentioned fungicide was applied at three concentrations: 0.1, 0.15 and 0.2%. As the standard fungicide Amistar 250 SC (biologically active substances BAS - azoxystrobin 250 g/dm3) was used. In every year of research work the four protective treatments were carried out. The estimation of infestation degree of Calendula officinalis leafs by the Erysiphe cichoracearum was made 5 times. Before each treatment four analysis were done, whereas the last analysis--the fifth one was executed after 10 days from the last protective spraying. According to the results, it was found that investigated preparations significant reduced the mean infestation degree of Calendula officinalis leafs by the Erysiphe cichoracearum compared to the control. The results pointed, that in protection of the mentioned plant by the powdery mildew the 0.2% dose of Zato 50WG showed the best suitability.

  3. Carotenoid isomerase is key determinant of petal color of Calendula officinalis.

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    Kishimoto, Sanae; Ohmiya, Akemi

    2012-01-02

    Orange petals of calendula (Calendula officinalis) accumulate red carotenoids with the cis-configuration at the C-5 or C-5' position (5-cis-carotenoids). We speculated that the orange-flowered calendula is a carotenoid isomerase (crtiso) loss-of-function mutant that impairs the cis-to-trans conversion of 5-cis-carotenoids. We compared the sequences and enzyme activities of CRTISO from orange- and yellow-flowered calendulas. Four types of CRTISO were expressed in calendula petals. The deduced amino acid sequence of one of these genes (CoCRTISO1) was different between orange- and yellow-flowered calendulas, whereas the sequences of the other three CRTISOs were identical between these plants. Analysis of the enzymatic activities of the CoCRTISO homologs showed that CoCRTISO1-Y, which was expressed in yellow petals, converted carotenoids from the cis-to-trans-configuration, whereas both CoCRTISO1-ORa and 1-ORb, which were expressed in orange petals, showed no activity with any of the cis-carotenoids we tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines linked closely to petal color. These data indicate that CoCRTISO1 is a key regulator of the accumulation of 5-cis-carotenoids in calendula petals. Site-directed mutagenesis showed that the deletion of Cys-His-His at positions 462-464 in CoCRTISO1-ORa and a Gly-to-Glu amino acid substitution at position 450 in CoCRTISO1-ORb abolished enzyme activity completely, indicating that these amino acid residues are important for the enzymatic activity of CRTISO.

  4. Usage of Calendula officinalis in the prevention and treatment of radiodermatitis: a randomized double-blind controlled clinical trial

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    Franciane Schneider

    2015-04-01

    Full Text Available OBJECTIVE To evaluate the efficacy of Calendula officinalis in relation to Essential Fatty Acids for the prevention and treatment of radiodermatitis. METHOD This is a randomized double-blind controlled clinical trial with 51 patients with head and neck cancer in radiotherapy treatment divided into two groups: control (27 and experimental (24. RESULTS There is statistically significant evidence (p-value = 0.0120 that the proportion of radiodermatitis grade 2 in Essential Fatty Acids group is higher than Calendula group. Through the Kaplan-Meier survival curve we observed that Essential Fatty Acids group has always remained below the Calendula group survival curve, due to the lower risk of developing radiodermatitis grade 1, which makes the usage of Calendula more effective, with statistical significance (p-value = 0.00402. CONCLUSION Calendula showed better therapeutic response than the Essential Fatty Acids in the prevention and treatment of radiodermatitis. Brazilian Registry of Clinical Trials: RBR-237v4b.

  5. Content of total carotenoids in Calendula officinalis L. from different countries cultivated in Estonia.

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    Raal, Ain; Kirsipuu, Kadri; Must, Reelika; Tenno, Silvi

    2009-01-01

    The aim of the present study was to investigate the content of total carotenoids in different collections of pot marigold (Calendula officinalis L.) inflorescences. Commercial seeds (42 samples) of C. officinalis were obtained from nine countries and cultivated in private gardens in three different counties of Estonia. The content of total carotenoids, determined spectrophotometrically (lambda=455 nm) varied in the investigated collections from 0.20 to 3.51%. The amount of pigments may depend on the type of plants, the importing company, the color of the ligulate and tubular florets, and the place and time of cultivation. For medicinal purposes, C. officinalis with brownish-yellow ligulate and tubular florets should be preferred. The best was found to be 'Golden Emperor' from Finland.

  6. Effect of water regime on the growth, flower yield, essential oil and proline contents of Calendula officinalis

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    SAMI ALI METWALLY

    2013-11-01

    Full Text Available Metwally SA,Khalid KA, Abou-Leila BH. 2013. Effect of water regime on the growth, flower yield, essential oil and proline contents of Calendula officinalis. Nusantara Bioscience 5: 63-67. The effects of water regime on the growth, content of essential oil and proline of Calendula officinalis L. plants were investigated. Water regimes of 75% of field water capacity increased certain growth characters [i.e. plant height (cm, leaf area (cm2, flower diameter (cm and spike stem diameter] and vase life (day. Water regime promoted the accumulation of essential oil content and its main components as well as proline contents.

  7. Actividad cicatrizante y ensayos de irritación de la crema de Calendula officinalis al 1%

    OpenAIRE

    2007-01-01

    El proceso de cicatrización de una herida en la piel involucra la compleja interacción de mu- chos tipos de células y ocurre como una cascada secuencial de procesos solapados e íntimamente relaciona- dos. A Calendula officinalis L. se le atribuyen efectos curativos sobre las lesiones de la piel como cicatrizante, por lo que se estudió el efecto cicatrizante e irritante ocular dérmico y oftálmico de una crema al 1% de Calendula officinalis L. sobre la cicatrización de heridas abiertas en piel ...

  8. Effect of Semiarid Environment on Some Nutritional and Antinutritional Attributes of Calendula (Calendula officinalis

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    Muhammad Iqbal

    2015-01-01

    Full Text Available Stressful environments have been shown to affect the metabolism in some plants. In the present study, we assessed whether semiarid environment (saline and saline alkaline soil could affect the nutritional (total proteins, phenolics and riboflavin contents, and catalase activity and antinutritional (hydrogen peroxide and malondialdehyde contents properties differently in different plant parts (leaves, flowers, and roots of calendula. Although salinity decreased plant biomass, it did not affect total protein and phenolics contents in the calendula. All plant parts were rich in riboflavin contents. However, plants grown under saline-alkali soil had relatively more riboflavin contents in the flowers. Salinity increased hydrogen peroxide (H2O2 concentration in the flowers and roots, whereas saline-alkali soil did not affect it. Plants exposed to both saline and saline-alkali soil had greater catalase activity in the flowers and leaves. Plants exposed to salinity had higher malondialdehyde (MDA contents in the flowers compared with nonsaline and saline-alkali conditions. Nonetheless, the possibility of safely using different parts of calendula as nutraceutical was in the order flower > root > leaf. Overall, the results suggested that plant can be grown in mild saline-alkali (EC ≤ 7; pH = 8.5 soils without affecting its nutraceutical properties.

  9. Angiogenic activity of Calendula officinalis flowers L. in rats Atividade angiogênica das flores da Calendula officinalis L. em ratos

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    Leila Maria Leal Parente

    2011-02-01

    Full Text Available Purpose: In this work, angiogenic activity of Calendula officinalis L. (Asteraceae ethanolic extract and dichloromethane and hexanic fractions were evaluated, considering medicinal properties, especially healing activity, are attributed to this plant. Methods: Models using 36 rats and 90 embryonated eggs were used to evaluate healing and angiogenic activities of extracts and fractions of the plant, through the induction of skin wounds and the chorioallantoic membrane, respectively. The effect of vascular proliferation was also tested from the study to verify the intensity of expression of vascular endothelial growth factor (VEGF in cutaneous wounds in rats. Results: The angiogenic activity of the extract and the fractions was evidenced in both experimental models. It was verified that this effect is not directly related to the expression of VEGF and it could be associated to other pro-angiogenic factors. Conclusion: The healing activity referred to C. officinalis is related, among other factors, to its positive effect on angiogenesis, characterized by the induction of neovascularization.Objetivo: Neste trabalho a atividade sobre a angiogênese do extrato etanólico (EEC e das frações diclorometano e hexânica das flores de Calendula officinalis L. (Asteraceae cultivada no Brasil foram avaliados, visto que propriedades medicinais têm sido atribuídas às flores da planta, destacando-se a atividade cicatrizante. Métodos: Modelos utilizando 36 ratos e 90 ovos embrionados foram usados para avaliar as atividades cicatrizante e angiogênica dos extratos e frações da planta, por meio da indução de feridas cutâneas e da membrana corioalantóide, respectivamente. O efeito proliferativo vascular foi também testado a partir do estudo imunoistoquímico, realizado para verificar a intensidade da expressão do fator de crescimento endotelial vascular (VEGF na derme de ratos. Resultados: A atividade angiogênica do extrato e das frações foi

  10. Componential profile and amylase inhibiting activity of phenolic compounds from Calendula officinalis L. leaves.

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    Olennikov, Daniil N; Kashchenko, Nina I

    2014-01-01

    An ethanolic extract and its ethyl acetate-soluble fraction from leaves of Calendula officinalis L. (Asteraceae) were found to show an inhibitory effect on amylase. From the crude extract fractions, one new phenolic acid glucoside, 6'-O-vanilloyl-β-D-glucopyranose, was isolated, together with twenty-four known compounds including five phenolic acid glucosides, five phenylpropanoids, five coumarins, and nine flavonoids. Their structures were elucidated based on chemical and spectral data. The main components, isoquercitrin, isorhamnetin-3-O-β-D-glucopyranoside, 3,5-di-O-caffeoylquinic acid, and quercetin-3-O-(6''-acetyl)-β-D-glucopyranoside, exhibited potent inhibitory effects on amylase.

  11. Effect of magnetic field on germination of two Calendula officinalis L. cultivars

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    Baradaranrad, A. Arouiee, H

    2016-12-01

    Full Text Available The main objective of this study is to determine the effects of magnetic treatment, in addition to the geomagnetic field, on germination of two Calendula officinalis L. cultivars, “Antares flashback” and “Gold star”. Groups of 50 seeds were exposed to 1505.2 and 2260.8 mT magnetic field generated by 1 mA electrical flow in 60 and 90 round coils for 6 and 24 hours. The selected germination parameters were germination rate and percentage, radicle and plumule length, seed vigor.

  12. Final report of the Cosmetic Ingredient Review Expert Panel amended safety assessment of Calendula officinalis-derived cosmetic ingredients.

    Science.gov (United States)

    Andersen, F Alan; Bergfeld, Wilma F; Belsito, Donald V; Hill, Ronald A; Klaassen, Curtis D; Liebler, Daniel C; Marks, James G; Shank, Ronald C; Slaga, Thomas J; Snyder, Paul W

    2010-01-01

    Calendula officinalis extract, C officinalis flower, C officinalis flower extract, C officinalis flower oil, and C officinalis seed oil are cosmetic ingredients derived from C officinalis. These ingredients may contain minerals, carbohydrates, lipids, phenolic acids, flavonoids, tannins, coumarins, sterols and steroids, monoterpenes, sesquiterpenes, triterpenes, tocopherols, quinones, amino acids, and resins. These ingredients were not significantly toxic in single-dose oral studies using animals. The absence of reproductive/developmental toxicity was inferred from repeat-dose studies of coriander oil, with a similar composition. Overall, these ingredients were not genotoxic. They also were not irritating, sensitizing, or photosensitizing in animal or clinical tests but may be mild ocular irritants. The Cosmetic Ingredient Review (CIR) Expert Panel concluded that these ingredients are safe for use in cosmetics in the practices of use and concentration given in this amended safety assessment.

  13. Calendula

    Science.gov (United States)

    ... On Ltd) that contains mullein, garlic, calendula, and St. John’s wort to the ear for 3 days reduces ear ... calamus, California poppy, catnip, hops, Jamaican dogwood, kava, St. John's wort, skullcap, valerian, yerba mansa, and others.

  14. Atividade antiinflamatória do granulado de Calendula officinalis L. e Matricaria recutita L.

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    L.R. Sartori

    2003-01-01

    Full Text Available Este trabalho descreve os resultados da atividade antiinflamatória de um granulado composto pelos extratos secos de Calendula officinalis L. e Matricaria recutita L. (GECOMR® - granulado encapsulado de Calendula officinalis e Matricaria recutita. Estas espécies são utilizadas na medicina popular devido às suas atividades antiinflamatória, antiúlcera gástrica e antiespasmódica. A redução do processo edematogênico nos grupos de animais tratados com o GECOMR foi observada para todos agentes inflamatórios testados (Carragenina, dextrana e histamina. Esta associação mostrou-se eficaz sobre os modelos de edema de pata induzidos pelas diferentes substâncias inflamatórias, com as doses de 100 e 250 mg.kg-1. A associação dos extratos hidroalcoólicos destas espécies medicinais não modificou a indicação isolada de cada uma.

  15. Phytochemical evaluation and in vitro antioxidant and photo-protective capacity of Calendula officinalis L. leaves

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    V.C.K.N. DEUSCHLE

    2015-01-01

    Full Text Available ABSTRACT The plant Calendula officinalis L. is widely applied due to its medicinal properties, which are mainly dermatological and ornamental. The goal of this study is to assess the phytochemical components in a hydroethanolic extract (HECO from the leaves of Calendula officinalis L. using UV-VIS spectrophotometry and thin layer chromatography (TLC, as well as to identify and quantify the components related to its antioxidant capacity employing high performance liquid chromatographic (HPLC. The antioxidant capacity evaluation was performed using the DPPH method for superoxide and hydroxyl radicals. The photo-protective capacity was evaluated by UVspectrophotometry in order to determine the in vitro Sun Protection Factor(SPF. The results show the plant’s strong antioxidant activity (DPPH and hydroxyl methods, which we believe to be related to the presence of flavonoids (24.67 mg/g, polyphenols (33.90 mg/g, condensed tannins (27.30 mg/g, and the amount of rutin (37.25 mg/g, and quercetin (6.09 mg/g found during the study. The HECO presented a good antioxidant capacity, most likely due to the polyphenols, flavonoids, and tannins in its contents. However, the obtained SPF of 1.89 ± 0.05 does not allow the plant to be classified as a stand-alone sunscreen, and more studies are needed in order to test its ability to enhance sunscreens in existing cosmetic formulations.

  16. Low-level laser therapy and Calendula officinalis in repairing diabetic foot ulcers

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    Ana Flávia Machado de Carvalho

    Full Text Available Abstract OBJECTIVE To evaluate the effects of low-level laser therapy isolated and associated with Calendula officinalis oil in treating diabetic foot ulcers. METHOD An experimental, randomized, controlled, prospective, interventional clinical case study using a quantitative approach. The sample consisted of 32 diabetic patients of both genders. Participants were randomly divided into four groups. Doppler Ultrasound evaluation of the Ankle-Brachial Index, brief pain inventory and analog pain scale were performed at baseline and after 30 days. RESULTS Reduced pain was observed in the Low-level laser therapy and Low-level laser therapy associated with Essential Fatty Acids groups (p<0.01. Regarding the Ankle-Brachial Index and Doppler Ultrasound, all groups remained stable. By analyzing lesion area reduction, Low-level laser therapy associated with Essential fatty acids group showed a significance of p=0.0032, and the Low-level laser therapy group showed p=0.0428. CONCLUSION Low-level laser therapy, performed alone or associated with the Calendula officinalis oil was effective in relieving pain and accelerating the tissue repair process of diabetic foot.

  17. Phenotypic and biochemical profile changes in calendula (Calendula officinalis L.) plants treated with two chemical mutagenesis.

    Science.gov (United States)

    El-Nashar, Y I; Asrar, A A

    2016-05-06

    Chemical mutagenesis is an efficient tool used in mutation-breeding programs to improve the vital characters of the floricultural crops. This study aimed to estimate the effects of different concentrations of two chemical mutagens; sodium azide (SA) and diethyl sulfate (DES). The vegetative growth and flowering characteristics in two generations (M1 and M2) of calendula plants were investigated. Seeds were treated with five different concentrations of SA and DES (at the same rates) of 1000, 2000, 3000, 4000, and 5000 ppm, in addition to a control treatment of 0 ppm. Results showed that lower concentrations of SA mutagen had significant effects on seed germination percentage, plant height, leaf area, plant fresh weight, flowering date, inflorescence diameter, and gas-exchange measurements in plants of both generations. Calendula plants tended to flower earlier under low mutagen concentrations (1000 ppm), whereas higher concentrations delayed flowering significantly. Positive results on seed germination, plant height, number of branches, plant fresh weight, and leaf area were observed in the M2-generation at lower concentrations of SA (1000 ppm), as well as at 4000 ppm DES on number of leaves and inflorescences. The highest total soluble protein was detected at the concentrations of 1000 ppm SA and 2000 ppm DES. DES showed higher average of acid phosphatase activity than SA. Results indicated that lower concentrations of SA and DES mutagens had positive effects on seed germination percentage, plant height, leaf area, plant fresh weight, flowering date, inflorescence diameter, and gas-exchange measurements. Thus, lower mutagen concentrations could be recommended for better floral and physio-chemical performance.

  18. The effects of extraction method on recovery rutin from Calendula officinalis L. (Asteraceae

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    Frederico Severino Martins

    2014-01-01

    Full Text Available Background: Calendula officinalis L. (Asteraceae is a Mediterranean specie, but in Europe and America it is cultivated for ornamental or medicinal purposes. This species is widely used for presenting activities, antiinflammatory antibacterial and antioxidant. However the therapeutic action is linked to the amount of assets of the extracted raw material. The extraction method of bioactive compounds is an important step in the manufacturing of herbal medicines, because secondary metabolites with therapeutic potential are usually found in small quantities in plant materials. Objective: Due the medical and commercial importance of C. officinalis, this study aimed to evaluate the impact of the extraction method on the quality of herbal extract and optimize the extraction of rutin from C. officinalis. Materials and Methods : The extraction of rutin was performed by ultrasound and shaker and the optimized conditions were determined by response surface methodology. Results: The results of ultrasound extraction assisted (UEA and maceration dynamic (MD showed that rutin yield ranged from 0.218 to 2.28% (w/w when extract by ultrasound and 0.1-1.44% by MD. The optimal extraction condition for rutin (2.48% to UEA or 1.46% to MD from C. officinalis by UEA or MD were a 19-22 min extraction, ethanol: water ratio of 35-40% and 0.05-0.056 mg/mL to raw material: solvent ratio. Conclusion: The UEA is more efficient to extraction rutin.

  19. PI3K-mediated proliferation of fibroblasts by Calendula officinalis tincture: implication in wound healing.

    Science.gov (United States)

    Dinda, Manikarna; Dasgupta, Uma; Singh, Namrata; Bhattacharyya, Debasish; Karmakar, Parimal

    2015-04-01

    Calendula officinalis, a member of the Asteraceae family, is a flowering plant and has been used for its antibacterial, antifungal, antiviral, antiinflammatory, anticancer and wound healing activity. The mode of action of C. officinalis tincture on wound healing is poorly understood. Here, we investigated the role of C. officinalis tincture (CDOT) on cell viability and wound closure. C. officinalis tincture stimulated both proliferation and migration of fibroblasts in a statistically significant manner in a PI3K-dependent pathway. The increase in phosphorylation of FAK (Tyr 397) and Akt (Ser 473) was detected after treatment of CDOT. Inhibition of the PI3K pathway by wortmannin and LY294002 decreased both cell proliferation and cell migration. HPLC-ESI MS revealed the presence of flavonol glycosides as the major compounds of CDOT. Altogether, our results showed that CDOT potentiated wound healing by stimulating proliferation and migration of fibroblast in a PI3K-dependent pathway, and the identified compounds are likely to be responsible for wound healing activity.

  20. Controversial effects of Calendula officinalis L. on Biochemical and Pathological Factors of Nephropathy in Diabetic Wistar Rats

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    Salehi

    2015-07-01

    Full Text Available Background Chronic hyperglycemia leads to microvascular and macrovascular complications such as diabetic nephropathy. Medicinal plants are good sources for finding new therapeutic chemicals to improve diabetes and relieve its symptoms. Objectives The purpose of the present study was to evaluate the effect of the hydroalcoholic extract (300 mg/kg of Calendula officinalis (marigold on blood biochemical profiles and histopathological changes in kidney of streptozotocin-induced diabetic rats. Materials and Methods Twenty male Wistar rats were divided to four groups: Normal control (NC, diabetic control (DC, normal C. officinalis (N+CO 300 mg/kg, and diabetic C. officinalis (D+CO 300 mg/kg. The rats were treated for a period of 13 weeks. Diabetes was induced by Streptozocin injection, intraperitoneally. Level of glucose, urea, creatinine and also total anti-oxidant capacity, malondialdehyde, total oxidant status in serum and histological alterations in the kidney were analyzed. Results Level of serum glucose, urea, creatinine, malondialdehyde and total oxidant status were increased in diabetic rats, whereas, total anti-oxidant capacity was decreased compared to the control animals. Also, histological findings confirmed the absence of integrity in glomerulus and mass infiltration in kidney tissue in diabetic rats compared to the normal controls. Calendula officinalis extract had no effect on blood glucose, but it decreased blood urea nitrogen and creatinine, total oxidant status and malondialdehyde while it increased total anti-oxidant capacity in the diabetic extract-treated group when compared to diabetic rats. Calendula officinalis could not prevent nephropathy changes in the diabetic rats. Conclusions Therefore, our results suggest that although administration of 300 mg/kg of Calendula officinalis extract showed salutary effects on anti-oxidant profile, yet its protective effects on anti-diabetic and regenerative properties on nephropathy were

  1. Role of priming technique in germination parameters of calendula (Calendula officinalis L. seeds

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    Karimi Mahnaz

    2016-01-01

    Full Text Available Seed priming is known for its promoting effects on seed and seedling properties under stress conditions such as salinity, temperature and drought stresses. Poor plant stand establishment is a common problem for marigold production. Therefore, an experiment was conducted to investigate the effect of different seed priming techniques on calendula seed germination and seedling enzyme activity. For this purpose, different seed priming techniques: hydropriming (distilled water, 24 and 72 h, osmopriming (0.02% or 0.04% KNO3 for 24 and 72 h and hormonal priming (100 or 500 mg L-1 GA3 for 24 and 72 h were assessed. Seeds without any treatment were considered as control. Compared with control, 100 mg L-1 GA3 and distilled water for 72 h showed the highest promoting effects on germination percentage. The highest vigor index (525.75 was related to hydropriming (for 24 h treatment. Also, the maximum root length was found with 0.02% KNO3 for 72 h. Catalase significantly increased in the primed seeds. The highest CAT activity and total soluble sugars were found in the seeds primed with 100 mg L-1 GA3 for 72 h. Moreover, the enzyme activity was significantly higher in the distilled water and KNO3 treatments compared with the untreated seeds.

  2. Growth, development and content of flavonoids in calendula (Calendula officinalis L.

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    Isabela Cristina Gomes Honório

    2016-01-01

    Full Text Available Flavonoids, the pharmaco-active compounds studied in this work, in general, are located in the shoots as natural pigments that protect the plants from damage by oxidizing agents. The experiment was conducted in the greenhouse of the Fitotecnia Department, Federal University of Viçosa, Minas Gerais Staty. The experimental design was completely randomized with five treatments and five replications. The treatments were the time of harvest: zero, three, six, nine, and twelve days after anthesis (DAA. The height of the plant (mm, stem diameter (mm, number of leaves and total flavonoid content (%, w w-1 were determined. We developed growth curves for calendula with growth behavior assessed through time for height, stem diameter and number of leaves. The time of the harvest season positively influenced the total flavonoid contents and the average productivity of flowers, and the highest content of flavonoids was found three DAA. The average productivity of the flowers and the accumulation of flavonoids were heterogeneous among harvest times (0, 3, 6, 9 and 12 days after anthesis. The highest yield of flavonoids was obtained three days after anthesis; therefore, a harvest of the inflorescences is recommended every three days.

  3. Healing acceleration of acetic acid-induced colitis by marigold (Calendula officinalis in male rats

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    Nader Tanideh

    2016-01-01

    Full Text Available Background/Aim: Ulcerative colitis (UC is a type of chronic inflammatory bowel disease with unknown etiology. Several therapeutic strategies such as consumption of medicinal plants have been used for its treatment. The aim of this study was to evaluate healing effects of Calendula officinalis hydroalcoholic extract in experimentally induced UC in rat. Materials and Methods: Ninety-six rats, weighing 200 ± 20 g, were randomly divided into eight equal groups. UC induced by 3% acetic acid and oral doses of C. officinalis extract, 1500 and 3000 mg/kg, and enema (gel 10% and 20% were given. Two groups as positive controls were given asacol (enema and oral mesalamine. Negative control groups were given normal saline and base gel. On days 3 and 7, intestinal histopathology and weight changes, plus oxidative stress indices including malondialdehyde (MDA level and myeloperoxidase (MPO activity were assayed. Results: A significant increase in the body weight of rats was seen in the group given C. officinalis extract 3000 mg/kg orally, oral mesalamine, and 20% intracolonic gel form of marigold extract compared with negative control and base gel groups during the experimental period. Acute inflammation and granular atrophy after UC induction were resolved completely completely by both 20% intracolonic gel and 3000 mg/kg orally. An increase in MPO activity and a decrease in MDA level in response to oral and intracolonic gel form of C. officinalis were observed 3 and and 7 days after treatment (P < 0.05. Conclusion: Our results indicate that oral and enema forms of hydroalcoholic extract of C. officinalis can be offered as are potential therapeutic agents for UC induced in rats.

  4. Evaluation of Calendula officinalis as an anti-plaque and anti-gingivitis agent

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    Mayur Sudhakar Khairnar

    2013-01-01

    Full Text Available Background: Calendula officinalis (C. officinalis, commonly known as pot marigold, is a medicinal herb with excellent antimicrobial, wound healing, and anti-inflammatory activity. Aim: To evaluate the efficacy of C. officinalis in reducing dental plaque and gingival inflammation. Materials and Methods: Two hundred and forty patients within the age group of 20-40 years were enrolled in this study with their informed consent. Patients having gingivitis (probing depth (PD ≤3 mm, with a complaint of bleeding gums were included in this study. Patients with periodontitis PD ≥ 4 mm, desquamative gingivitis, acute necrotizing ulcerative gingivitis (ANUG, smokers under antibiotic coverage, and any other history of systemic diseases or conditions, including pregnancy, were excluded from the study. The subjects were randomly assigned into two groups - test group (n = 120 and control group (n = 120. All the test group patients were advised to dilute 2 ml of tincture of calendula with 6 ml of distilled water and rinse their mouths once in the morning and once in the evening for six months. Similarly, the control group patients were advised to use 8 ml distilled water (placebo as control mouthwash and rinse mouth twice daily for six months. Clinical parameters like the plaque index (PI, gingival index (GI, sulcus bleeding index (SBI, and oral hygiene index-simplified (OHI-S were recorded at baseline (first visit, third month (second visit, and sixth month (third visit by the same operator, to rule out variable results. During the second visit, after recording the clinical parameters, each patient was subjected to undergo a thorough scaling procedure. Patients were instructed to carry out regular routine oral hygiene maintenance without any reinforcement in it. Results: In the absence of scaling (that is, between the first and second visit, the test group showed a statistically significant reduction in the scores of PI, GI, SBI (except OHI-S (P < 0

  5. Acute and subchronic oral toxicities of Calendula officinalis extract in Wistar rats.

    Science.gov (United States)

    Lagarto, Alicia; Bueno, Viviana; Guerra, Isbel; Valdés, Odalys; Vega, Yamile; Torres, Leonid

    2011-05-01

    We have studied the acute and subchronic oral toxicities of Calendula officinalis extract in male and female Wistar rats. A single acute C. officinalis extract dose of 2000 mg/kg dissolved in distilled water was administered by oral gavage for acute toxicity. Subchronic doses of 50, 250 and 1000 mg/kg/day were administered in drinking water. The major toxicological endpoints examined included animal body weight, water and food intake, selected tissue weights, and histopathological examinations. In addition, we examined blood elements: hematocrit, hemoglobin concentration, erythrocyte count, total and differential leukocyte count and blood clotting time and blood chemistry: glucose, total cholesterol, urea, total proteins, alkaline phosphatase, alanine aminotransferase (ALT) and aspartate aminotransferase (AST). In the acute study, there were no mortality and signs of toxicity. In the subchronic study, several of the blood elements were significantly affected in males and females after 90 days; hemoglobin, erythrocytes, leukocytes and blood clotting time. For blood chemistry parameters, ALT, AST and alkaline phosphatase were affected. Histopathological examination of tissues showed slight abnormalities in hepatic parenchyma that were consistent with biochemical variations observed. These studies indicate that the acute and subchronic toxicities of C. officinalis extract are low.

  6. Antifungal activity of the essential oil from Calendula officinalis L. (asteraceae growing in Brazil Atividade antifúngica do óleo essencial da Calendula officinalis cultivada no Brasil

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    Zilda Cristiane Gazim

    2008-03-01

    Full Text Available This study tested in vitro activity of the essential oil from flowers of Calendula officinalis using disk-diffusion techniques. The antifungal assay results showed for the first time that the essential oil has good potential antifungal activity: it was effective against all 23 clinical fungi strains tested.Neste estudo foi avaliada a atividade antifúngica do óleo essencial, obtido das flores de Calendula officinalis utilizando-se técnica de difusão em discos. Os resultados dos ensaios antifúngicos mostraram pela primeira vez, que o óleo essencial tem um grande potencial antifúngico, pois foi efetivo contra todas as 23 amostras clínicas de fungos testados.

  7. Subacute effect of cigarette smoke exposure in rats: protection by pot marigold (Calendula officinalis L.) extract.

    Science.gov (United States)

    Ozkol, Halil; Tülüce, Yasin; Koyuncu, Ismail

    2012-02-01

    This study was carried out to determine the preventive effect of Calendula officinalis L. (pot marigold) on rats exposed to cigarette smoke (CS). Rats were divided into three groups as control, CS and CS + pot marigold (PM). The rats in the CS and CS + PM groups were subjected to CS for 1 h twice a day for 23 days. PM (100 mg/kg body weight) was given to rats in the CS + PM group by gavage, 1 h before each administration period. While malondialdehyde, protein carbonyl contents and reduced glutathione level of the CS group increased, their levels diminished by PM administration. In addition, glutathione peroxidase (GPx), superoxide dismutase activities and β-carotene, vitamins A and C levels decreased in the CS group compared to control, however activities of these enzymes and concentration of vitamins were elevated by PM supplementation. This investigation showed that administration of PM supplied relative protection against subacute CS-induced cell injury.

  8. Componential Profile and Amylase Inhibiting Activity of Phenolic Compounds from Calendula officinalis L. Leaves

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    Daniil N. Olennikov

    2014-01-01

    Full Text Available An ethanolic extract and its ethyl acetate-soluble fraction from leaves of Calendula officinalis L. (Asteraceae were found to show an inhibitory effect on amylase. From the crude extract fractions, one new phenolic acid glucoside, 6′-O-vanilloyl-β-D-glucopyranose, was isolated, together with twenty-four known compounds including five phenolic acid glucosides, five phenylpropanoids, five coumarins, and nine flavonoids. Their structures were elucidated based on chemical and spectral data. The main components, isoquercitrin, isorhamnetin-3-O-β-D-glucopyranoside, 3,5-di-O-caffeoylquinic acid, and quercetin-3-O-(6′′-acetyl-β-D-glucopyranoside, exhibited potent inhibitory effects on amylase.

  9. Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis Utilização de técnicas de cultura de tecidos na proliferação de brotos e enraizamento de Calendula officinalis

    OpenAIRE

    Cristiane Pimentel Victório; Celso Luiz Salgueiro Lage; Alice Sato

    2012-01-01

    The high demand for plant material from Calendula officinalis in the production of herbal medicines and cosmetics, turns the technique of plant-tissue culture into one of the alternatives for the improvement of crops over a short period of time. A protocol for tissue culture was developed from segments of seedlings of C. officinalis, in order to improve the proliferation of shoots and roots. We used a Murashige and Skoog (MS½N) medium, reduced to half the concentrations of NH4NO3 and KNO3 to ...

  10. Fatty acid composition of lipids in pot marigold (Calendula officinalis L. seed genotypes

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    Dulf Francisc V

    2013-01-01

    Full Text Available Abstract Background Calendula officinalis L. (pot marigold is an annual aromatic herb with yellow or golden-orange flowers, native to the Mediterranean climate areas. Their seeds contain significant amounts of oil (around 20%, of which about 60% is calendic acid. For these reasons, in Europe concentrated research efforts have been directed towards the development of pot marigold as an oilseed crop for industrial purposes. Results The oil content and fatty acid composition of major lipid fractions in seeds from eleven genotypes of pot marigold (Calendula officinalis L. were determined. The lipid content of seeds varied between 13.6 and 21.7 g oil/100 g seeds. The calendic and linoleic acids were the two dominant fatty acids in total lipid (51.4 to 57.6% and 28.5 to 31.9% and triacylglycerol (45.7 to 54.7% and 22.6 to 29.2% fractions. Polar lipids were also characterised by higher unsaturation ratios (with the PUFAs content between 60.4 and 66.4%, while saturates (consisted mainly of palmitic and very long-chain saturated fatty acids were found in higher amounts in sterol esters (ranging between 49.3 and 55.7% of total fatty acids. Conclusions All the pot marigold seed oils investigated contain high levels of calendic acid (more than 50% of total fatty acids, making them favorable for industrial use. The compositional differences between the genotypes should be considered when breeding and exploiting the pot marigold seeds for nutraceutical and pharmacological purposes.

  11. Potential of extracts from Saponaria officinalis and Calendula officinalis to modulate in vitro rumen fermentation with respect to their content in saponins.

    Science.gov (United States)

    Budan, Alexandre; Bellenot, Denis; Freuze, Ingrid; Gillmann, Louisa; Chicoteau, Pierre; Richomme, Pascal; Guilet, David

    2014-01-01

    Saponins have the potential to favorably modulate rumen fermentation, but there is generally a lack of the chemical structures associated with the described effects. The activity of extracts from Calendula officinalis and Saponaria officinalis in the rumen was evaluated in vitro. The S. officinalis root extract, reduced CH₄ production by 8.5% and increased total VFA concentration by 25.2%. C. officinalis and S. officinalis root extracts and the S. officinalis aerial part extract decreased the acetate to propionate ratio from 8.6 to 17.4%, according to the extract. An HPLC-ELSD analysis indicated that the saponin content ranged from 43.6 to 57.6 mg/g of dry matter (DM) in the C. officinalis extracts and from 224.0 to 693.8 mg/g of DM in the S. officinalis extracts, expressed as the hederacoside C equivalent. Identification of the saponin compounds present in the extracts by HPLC-MS(n) suggested that the saponin profile modulated the biological activities, showing the importance of determining the structure of saponins when evaluating extracts.

  12. Anti-inflammatory activity of flower extract of Calendula officinalis Linn. and its possible mechanism of action.

    Science.gov (United States)

    Preethi, Korengath Chandran; Kuttan, Girija; Kuttan, Ramadasan

    2009-02-01

    Calendula officinalis flower extract possessed significant anti-inflammatory activity against carrageenan and dextran-induced acute paw edema. Oral administration of 250 and 500 mg/kg body weight Calendula extract produced significant inhibition (50.6 and 65.9% respectively) in paw edema of animals induced by carrageenan and 41.9 and 42.4% respectively with inflammation produced by dextran. In chronic anti-inflammatory model using formalin, administration of 250 and 500 mg/kg body weight Calendula extract produced an inhibition of 32.9 and 62.3% respectively compared to controls. TNF-alpha production by macrophage culture treated with lipopolysaccharide (LPS) was found to be significantly inhibited by Calendula extract. Moreover, increased levels of proinflammatory cytokines IL- 1beta, IL-6, TNF-alpha and IFN-gamma and acute phase protein, C- reactive protein (CRP) in mice produced by LPS injection were inhibited significantly by the extract. LPS induced cyclooxygenase-2 (Cox-2) levels in mice spleen were also found to be inhibited by extract treatment. The results showed that potent anti-inflammatory response of C. officinalis extract may be mediated by the inhibition of proinflammatory cytokines and Cox-2 and subsequent prostaglandin synthesis.

  13. INVESTIGATION OF DIURETIC ACTIVITY OF PHYTOPREPARATIONS OF CALENDULA OFFICINALIS L. FLOWERS

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    V. A. Kurkin

    2016-01-01

    Full Text Available The flowers of the pot marigold (Calendula officinalis L. are part of combination herbal medicinal preparations which applied in the treatment of diseases of the hepatobiliary system, gastrointestinal tract, diseases of kidneys and the urinary tract, such as «Fitonephrol» («Urological tea». Calendula flowers make a contribution in the total therapeutic activity of «Urological tea» as a component which has anti-inflammatory and reparative properties. As is widely known that phytopharmaceuticals usually combine the variety of therapeutic activity and softness of pharmacological effects along with the absence of a significant number of side effects and contraindications which is extremely important in the treatment of chronic pathologies. The main purpose was to study diuretic activity of phytopreparations of pot marigold.Materials and methods. Investigations of discovering of diuretic action of pot marigold’s phytopreparations were carried out on white rats of both sexes weighing 200–220 g (8 sets of experiments. Each experimental group consisted of ten animals. The effect of pot marigold medicines at a dose of 50 mg/kg was carried out background the administered intragastrically at 3% aqueous load of 4 and 24 hours of the experiment. There are such drugs as a subjects to examination: calendula tincture (1 : 10 (industrial production, calendula tincture (1 : 5 and calendula liquid extract (1 : 2 of 70% alcohol, obtained at the Department of Pharmacognosy with Botany and Bases of Phytotherapy, at a dose of 50 mg/kg. Animals were housed in the vivarium on a standard diet with free access to water. The day before the experiment the rats received intragastric water load in the amount of 3% of body weight. On the day of the experiment the animals of the control group was administered water-alcohol load and experienced – intragastrically drug dose of 50 mg/kg in an identical volume of water. As a control was used 70% alcohol. Animals were

  14. Application of the threshold of toxicological concern approach for the safety evaluation of calendula flower (Calendula officinalis) petals and extracts used in cosmetic and personal care products.

    Science.gov (United States)

    Re, T A; Mooney, D; Antignac, E; Dufour, E; Bark, I; Srinivasan, V; Nohynek, G

    2009-06-01

    Calendula flower (Calendula officinalis) (CF) has been used in herbal medicine because of its anti-inflammatory activity. CF and C. officinalis extracts (CFE) are used as skin conditioning agents in cosmetics. Although data on dermal irritation and sensitization of CF and CFE's are available, the risk of subchronic systemic toxicity following dermal application has not been evaluated. The threshold of toxicological concern (TTC) is a pragmatic, risk assessment based approach that has gained regulatory acceptance for food and has been recently adapted to address cosmetic ingredient safety. The purpose of this paper is to determine if the safe use of CF and CFE can be established based upon the TTC class for each of its known constituents. For each constituent, the concentration in the plant, the molecular weight, and the estimated skin penetration potential were used to calculate a maximal daily systemic exposure which was then compared to its corresponding TTC class value. Since the composition of plant extracts are variable, back calculation was used to determine the maximum acceptable concentration of a given constituent in an extract of CF. This paper demonstrates the utility and practical application of the TTC concept when used as a tool in the safety evaluation of botanical extracts.

  15. Study regarding the effect of calendula officinalis cream in healing of pressure sores

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    Ravanbakhsh Esmaili

    2008-01-01

    Full Text Available (Received 13 July, 2008 ; Accepted 29 Oct, 2008 Abstract Background and Purpose: Pressure sore is common among patients with prolonged stay in hospitals or homes. Treatment of such sores is costly and performed by various procedures. Considering the effective role of calendula officinalis in treatment of sores, ulcers and cutaneous inflammations, this study is conducted to determine the effect of this cream in healing pressure sores.Materials and methods: This is a pre/post comparative clinical trial done on 20 patients with pressure sores who received the recommended treatment.The condition of patients such as, duration of pressure sore, and the extent of the sore were recorded. The sore was washed with normal saline, dried, followed by applying calendula cream three times a day for a duration of 4 weeks. Each week, the extent and rate of healing was observed and recorded.Results: The rate of healing in patients under our study was 56.6%. Majority (55% of patients had partial healing. The time of healing was in weeks 3.5±1.2 (P<0.001. The complete healing occurred in the 3rd and 4th week, subsequent to the beginning of the treatment.Conclusion: Considering the availability of the cream and its affordability, it can be used in the treatment of pressure sores. In addition, it can be used as a drug for treatment of patients who are in hospitals and/or in their homes. J Mazand Univ Med Sci 2008; 18(66:19-25(Persian

  16. Extracts from Calendula officinalis offer in vitro protection against H2 O2 induced oxidative stress cell killing of human skin cells.

    Science.gov (United States)

    Alnuqaydan, Abdullah M; Lenehan, Claire E; Hughes, Rachel R; Sanderson, Barbara J

    2015-01-01

    The in vitro safety and antioxidant potential of Calendula officinalis flower head extracts was investigated. The effect of different concentrations (0.125, 0.5, 1.0, 2.0 and 5.0% (v/v)) of Calendula extracts on human skin cells HaCaT in vitro was explored. Doses of 1.0% (v/v) (0.88 mg dry weight/mL) or less showed no toxicity. Cells were also exposed to the Calendula extracts for either 4, 24 or 48 h before being exposed to an oxidative insult (hydrogen peroxide H2 O2 ) for 1 h. Using the MTT cytotoxicity assay, it was observed that two independent extracts of C. officinalis gave time-dependent and concentration-dependent H2 O2 protection against induced oxidative stress in vitro using human skin cells. Pre-incubation with the Calendula extracts for 24 and 48 h increased survival relative to the population without extract by 20% and 40% respectively following oxidative challenge. The antioxidant potential of the Calendula extracts was confirmed using a complimentary chemical technique, the DPPH(●) assay. Calendula extracts exhibited free radical scavenging abilities. This study demonstrates that Calendula flower extracts contain bioactive and free radical scavenging compounds that significantly protect against oxidative stress in a human skin cell culture model.

  17. The performance of young pigs fed different amounts of marigold (Calendula officinalis) meal; a pilot study

    NARCIS (Netherlands)

    Hindle, V.A.; Mathijssen-Kamman, A.A.; Stockhofe, N.; Cone, J.W.

    2002-01-01

    Hexane-extracted calendula meal was tested in an acceptance trial with pigs to determine their response to calendula meal. Performance parameters included feed intake, daily growth and post mortem histopathological examination of vital organs. Although calendula meal showed potential as a ration ing

  18. Bioefficacy of Calendula officinalis Linn. (Asteraceae extracts in the control of Spodoptera litura Fabricus (Noctuidae: Lepidoptera under laboratory conditions.

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    Doddamane Manjulakumari

    2012-08-01

    Full Text Available The effect of Calendula officinalis L on the nutritional physiological parameters of Spodoptera litura Fab larvae were studied by treating with leaf and flower extracts in various solvents. Protease and amylase enzyme activities along with their respective substrates were assayed in hemolymph and midgut of treated and untreated larvae. Irrespective of tissues, all the extracts showed significant reduction in the biochemical profile compared to controls. The most significant change was observed in chloroform and petroleum ether leaf and flower extracts treated larvae compared to other extracts suggesting their components affect nutritional physiology of S.litura. Hence, Calendula extracts can not only be used to treat human ailments but also as an effective botanical pesticide.

  19. Antibacterial and antiparasitic activity of oleanolic acid and its glycosides isolated from marigold (Calendula officinalis).

    Science.gov (United States)

    Szakiel, Anna; Ruszkowski, Dariusz; Grudniak, Anna; Kurek, Anna; Wolska, Krystyna I; Doligalska, Maria; Janiszowska, Wirginia

    2008-11-01

    The antibacterial and antiparasitic activities of free oleanolic acid and its glucosides and glucuronides isolated from marigold (Calendula officinalis) were investigated. The MIC of oleanolic acid and the effect on bacterial growth were estimated by A600 measurements. Oleanolic acid's influence on bacterial survival and the ability to induce autolysis were measured by counting the number of cfu. Cell morphology and the presence of endospores were observed under electron and light microscopy, respectively. Oleanolic acid inhibited bacterial growth and survival, influenced cell morphology and enhanced the autolysis of Gram-positive bacteria suggesting that bacterial envelopes are the target of its activity. On the other hand, glycosides of oleanolic acid inhibited the development of L3 Heligmosomoides polygyrus larvae, the infective stage of this intestinal parasitic nematode. In addition, both oleanolic acid and its glycosides reduced the rate of L3 survival during prolonged storage, but only oleanolic acid glucuronides affected nematode infectivity. The presented results suggest that oleanolic acid and its glycosides can be considered as potential therapeutic agents.

  20. Reproductive assessment of hydroalcohol extract of Calendula officinalis L. in Wistar rats.

    Science.gov (United States)

    Silva, Erick J R; Costa-Silva, João H; Evêncio, Liriane B; Fraga, Maria do Carmo C A; Coelho, Maria Cristina O C; Wanderley, Almir G

    2009-10-01

    The present study was conducted to evaluate the effects of the administration of a hydroalcohol extract of Calendula officinalis L. flowers (HAE) on the reproductive function of Wistar rats. Four groups of adult male rats were treated orally with HAE at doses of 0, 0.25, 0.5 and 1.0 g/kg for 60 consecutive days. From day 53 to 60 of treatment, rats were mated with untreated and fertile female rats. Reproductive parameters including testicular morphology, reproductive organ weights, fertility index and offspring viability were evaluated. In another protocol, groups of pregnant rats were treated orally with the same doses of HAE from days 1 to 6 (preimplantation period), 7 to 14 (organogenic period) or 15 to 19 (fetal period) of pregnancy. On day 20 of pregnancy, rats were killed for evaluation of maternal and fetal parameters. The results showed that the treatment with HAE did not affect male reproductive parameters. Besides, it was non-toxic in the preimplantation and organogenic periods of pregnancy. However, the HAE induced a decrease of the maternal weight gain when administered during the fetal period. In conclusion, the HAE did not affect male fertility nor had toxic effects in early and middle periods of pregnancy. However, the HAE caused maternal toxicity when administered during the fetal period of pregnancy.

  1. Antioxidant polyphenol and flavone contents in correlation with cultivation technology for Calendula officinalis L.

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    Ersilia ALEXA

    2009-05-01

    Full Text Available During the last two decades there has been a strong trend to returning to plant therapy. Research highlight polyphenol and flavone content of Calendula officinalis L playing an important role in protecting man’s health. Natural antioxidants are spread particularly in the plant kingdom[2]. Bio-flavones and polyphenols are man’s main allies in his fight against all threats to body’s health, including viruses, cancer,, toxic substances and micro-organisms. Antioxidant action of polyphenols is largely used in medicine and pharmacy as deactivating agents of ultraviolet radiation and metal ions. These substances decompose peroxides and inhibit the forming action of free radicals, thus hindering disease appearance [3]. The paper aims to establish that the yield capacity of the analysed cultivars was significant influenced by the cultivation technology. Sowing densities is one of the most important technological step for marigold. Fertilisation also had great influence on yield capacity. Concerning the polyphenol content and the flavones content we can observe that the technology had great impact on their quantity and quality, so in order to obtain best results we should take in consideration the fact that is vital to improve the cultivation technology for marigold. The quality of marigold yield is given by the content of active elements from the inflorescences. From these elements we study in particularly the polyphenols and the flavones.

  2. Production of oleanolic acid glycosides by hairy root established cultures of Calendula officinalis L.

    Science.gov (United States)

    Długosz, Marek; Wiktorowska, Ewa; Wiśniewska, Anita; Pączkowski, Cezary

    2013-01-01

    In order to initiate hairy root culture initiation cotyledons and hypocotyls of Calendula officinalis L. were infected with Agrobacterium rhizogenes strain ATCC 15834 or the same strain containing pCAMBIA 1381Z vector with β-glucuronidase reporter gene under control of promoter of NIK (Nematode Induced Kinase) gene. The efficiency of induction of hairy roots reached 33.8% for cotyledons and 66.6% for hypocotyls together for both transformation experiments. Finally, eight control and nine modified lines were established as a long-term culture. The hairy root cultures showed the ability to synthesize oleanolic acid mainly (97%) as glycosides; control lines contained it at the average 8.42 mg · g(-1) dry weight in tissue and 0.23 mg · dm(-3) in medium; modified lines: 4.59 mg · g(-1) for the tissue, and 0.48 mg · dm(-3) for the medium. Additionally lines showed high positive correlation between dry/fresh weight and oleanolic acid concentration in tissue. Using the Killiani mixture in acidic hydrolysis of oleanolic acid glycosides released free aglycones that were partially acetylated in such conditions.

  3. Magnetic molecularly imprinted polymer for the selective extraction of quercetagetin from Calendula officinalis extract.

    Science.gov (United States)

    Ma, Run-Tian; Shi, Yan-Ping

    2015-03-01

    A new magnetic molecularly imprinted polymers (MMIPs) for quercetagetin was prepared by surface molecular imprinting method using super paramagnetic core-shell nanoparticle as the supporter. Acrylamide as the functional monomer, ethyleneglycol dimethacrylate as the crosslinker and acetonitrile as the porogen were applied in the preparation process. Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD) and Vibrating sample magnetometer (VSM) were applied to characterize the MMIPs, and High performance liquid chromatography (HPLC) was utilized to analyze the target analytes. The selectivity of quercetagetin MMIPs was evaluated according to their recognition to template and its analogues. Excellent binding for quercetagetin was observed in MMIPs adsorption experiment, and the adsorption isotherm models analysis showed that the homogeneous binding sites were distributed on the surface of the MMIPs. The MMIPs were employed as adsorbents in solid phase extraction for the determination of quercetagetin in Calendula officinalis extracts. Furthermore, this method is fast, simple and could fulfill the determination and extraction of quercetagetin from herbal extract.

  4. PARAMETERS OF THE IMMUNOLOGICAL PROFILE IN CHICKENS TREATED WITH A CALENDULA OFFICINALIS EXTRACTION

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    C DUMITRU

    2002-11-01

    Full Text Available Tests were carried out on thirty, 42 days old Rock x Cornish chickens, divided into three experimental groups: I - control injected with saline, II - injected with a Calendula officinalis extraction supplemented with Bayol and III - treated with Bayol. The effects of the in vivo treatments and simultaneous antigen priming (0.5 ml of a 5 % suspension of SRBC on their humoral (lysozyme, anti-SRBC antibodies and cell-mediated (leucocyte blast transformation responses were investigated. Lysozyme (51.50 ± 32.30, 56.80 ± 41.27, 29.50 ± 22.73 μg/ml(p<0.05, and anti-SRBC antibody titers (2.35 ± 0.86, 2.05 ± 0.65 and 1.90 ± 0.55 were the lowest in Bayol treated group. Spontaneous stimulation index was positively influenced by the in vivo Bayol therapy (57.20 ± 8.88 per cent, when compared to that recorded for the other groups (31.06 ± 18.93 group I, 33.37 ± 19.26 group II.

  5. Actividad Antioxidante de extractos de Calendula officinalis asociados a soportes de colágeno tipo I

    OpenAIRE

    Flórez Cabrera, Adriana Matilde

    2014-01-01

    En este trabajo se evaluó la actividad antioxidante de los metabolitos secundarios presentes en dos extractos, oleoso e hidroglicólico, de flores de Calendula officinalis liberados de forma controlada por soportes de colágeno tipo I con micropartículas de gelatina-colágeno desarrollados mediante ingeniería de tejidos, con el fin determinar su posible aplicación en heridas cutáneas crónicas con elevadas concentraciones de especies reactivas de oxígeno. Se realizaron pruebas de citotoxicida...

  6. An investigation of CO2 extraction of marigold (Calendula officinalis L.

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    LIDIJA PETROVIC

    2007-04-01

    Full Text Available Essential oil content (0.05 % of marigold (Calendula officinalis L. was determined using the official steam distillation procedure. High-pressure CO2 extraction of plant material under supercritial (100, 200 and 300 bar and 40 °C and subcritical (60, 90 and 120 bar and 15 °C conditions for 3 h was investigated. It was found that the increase in the pressure promoted an increase in the yield. The essential oil contents obtained from the investigated CO2 extracts by steam distillation were significantly higher (1.52–2.70 times and increased with pressure.Major constituents of the oil, identified using GC-MS and GC-FID, were a-cadinol (26.54 %, T-cadinol and T-muurolol (9.80 %, g-cadinene (2.99 %, hexadecanoic acid (2.95 %, and ledane (2.45 %. In addition, the essential oils of the CO2 extracts contained d-cadinene (6.50–19.87 % under supercritical and 16.09–19.41 % under subcritical conditions, which was not found in the essential oil obtained from the plant by steam distillation. The extraction kinetics was investigated at 200 bar and 40 °C. The total extract obtained after 10 h of extraction was 6.54 % and essential oil content in it, refering to plant material, was 0.209 %, which is 4.16 times more than the one determined by the standard steam distillation procedure.

  7. Thin-layer chromatography analysis and scavenging activity of marigold (Calendula officinalis L extracts

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    Ćetković Gordana S.

    2003-01-01

    Full Text Available The methanol, petroleum ether, chloroform, ethyl acetate, n-butanol and water extracts were obtained by extraction of marigold flower (Calendula officinalis L. The content of total phenolic compounds, determined by UV spectrophotometric method using the Folin-Ciocalteu reagent, was 15.12 mg/g. The content of total flavonoids, determined by UV spectrophotometric method according to Markham, was 5.13 mg/g. Qualitative determination of phenolic compounds in the extracts was performed by one- and two-dimensional thin-layer chromatography (TLC procedures. The results of one- and two-dimensional TLC analyses showed that different flavonoids and phenolic acids were present in the investigated extracts. The greatest number of flavonoids (rutin, quercetin and some unidentified flavonoid glycosides and phenolic acids (chlorogenic, caffeic, coumaric and vanillic acid were deteminated in methanol extract. The influence of marigold extracts, in concentration range 0.6-1.2 mg/mL, on 2,2’-diphenyl-1-picrylhydrazyl (DPPH free radicals was investigated by electron spin resonance (ESR spectroscopy. All extracts showed scavenging activity (SA in the following order: ethyl acetate > n-butanol > methanol > water > chloroform > petroleum ether. The SA increased with increasing concentration of extracts. The ethyl acetate and n-butanol extracts exibited the most significant SA. These extracts in concentration of 1.2 mg/mL eliminated completely DPPH radicals. The lowest SA had chloroform and petroleum ether extracts (in concentration of 0.6 mg/mL SA=0%. The SA of marigold extracts is attributed to its hydrogen-donating ability and scavenging effect.

  8. Antimicrobial activity of Calendula officinalis petal extracts against fungi, as well as Gram-negative and Gram-positive clinical pathogens.

    Science.gov (United States)

    Efstratiou, Efstratios; Hussain, Abdullah I; Nigam, Poonam S; Moore, John E; Ayub, Muhammad A; Rao, Juluri R

    2012-08-01

    The aim of the present study was to assess the antimicrobial activity of methanol and ethanol extracts of pot marigold (Calendula officinalis) petals against clinical pathogens. The antimicrobial potential of C. officinalis extracts was evaluated against a panel of microorganisms isolated from patients at the Belfast City Hospital (BCH), including bacteria and fungi, using disc diffusion assay. Methanol extract of C. officinalis exhibited better antibacterial activity against most of the bacteria tested, than ethanol extract. Both methanol and ethanol extracts showed excellent antifungal activity against tested strains of fungi, while comparing with Fluconazole.

  9. A dual and opposite effect of Calendula officinalis flower extract: chemoprotector and promoter in a rat hepatocarcinogenesis model.

    Science.gov (United States)

    Barajas-Farias, L M; Pérez-Carreón, J I; Arce-Popoca, E; Fattel-Fazenda, S; Alemán-Lazarini, L; Hernández-García, S; Salcido-Neyoy, M; Cruz-Jiménez, F G; Camacho, J; Villa-Treviño, S

    2006-02-01

    Calendula officinalis extracts have protective and cytotoxic effects. We previously reported the dual activity of C. officinalis in primary rat hepatocyte cultures treated with N-nitrosodiethylamine. At nM concentrations it was anti-genotoxic while at microM concentrations it exhibited genotoxic effects. Here we tested the activity of Calendula officinalis in vivo in male Fischer 344 rats initiated with N-nitrosodiethylamine, promoted with 2-acetylaminofluorene, and 70 % partially hepatectomized. Liver gamma-glutamyltranspeptidase positively altered hepatocyte foci 25 days after initiation were our end point. The protective effect of C. officinalis started at 0.1 mg/kg concentration, increased at 0.5 mg/kg and reached its maximum at 2.5 mg/kg, when it decreased the area and number of altered foci by 55 % and 49 %, respectively, in comparison with rats treated only with carcinogen. At 5 mg/kg the number and area of altered hepatocyte foci were still lower, but almost reached the figures of carcinogen-treated rats. Ten and 20 mg/kg doses produced a notorious increment in the area and number of altered hepatic foci, and at 40 mg/kg of extract the increment was 40 % and 53 %, respectively. Additionally, when 2-acetylaminofluorene was substituted by a 40 mg/kg C. officinalis extract, a promoting effect was observed with increments of 175 % and 266 % in area and number of altered hepatocyte foci with respect to controls. When N-nitrosodiethylamine was substituted by 40 mg/kg of extract, the latter did not show initiator activity. In summary, we showed a protecting activity of C. officinalis at low doses, but doses above 10 mg/kg increased altered hepatocyte foci. This dual effect is an example of the phenomenon of hormesis. Furthermore, 40 mg/kg of dry weight extract administered instead of 2-acetylaminofluorene induced a clear promoting activity. These in vivo results are similar and consistent with those reported by us in primary rat liver cell cultures.

  10. Impact of exogenous salicylic acid on growth and ornamental characteristics of calendula (Calendula officinalis L. under salinity stress

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    Bayat H.

    2012-04-01

    Full Text Available Application of salicylic acid (SA as a phytohormone has been increased due to resistance to stresses such as salt stress. Pot experiments were conducted to determine the effect of exogenous salicylic acid application on growth and ornamental characteristics of calendula grown under salt stress and greenhouse conditions. For this purpose a factorial experiment based on completely randomized design was conducted with 3 levels of SA (0 (control, 1, 2 mM and 3 levels of NaCl (0, 100 and 200 mM with 4 replications. At flowering stage, SA was applied with spraying two times in two week intervals. NaCl was also applied as drench (200 ml per pot in two day intervals. The results showed that salinity decreased the growth, Chlorophyll reading values, flower number per plant and flower diameter. However, foliar applications of SA resulted in greater root, shoot and total dry weight, plant height and leaf area of calendula plants under salt stress. The highest chlorophyll reading values was obtained from 2.00 mM SA application in all NaCl treatments. Salinity decreased number of flower per plant and flower diameter as ornamental characteristics; however SA increased them under salinity stress. Plants treated with 1.00 mM SA had the highest flower diameter at 100 and 200 mM of NaCl. Electrolyte leakage increased by salinity, however foliar application of SA significantly reduced electrolyte leakage under salt stress. Based on the present results, foliar application of SA treatments can ameliorate the negative effects of salinity on the growth and ornamental characteristics of calendula plants.

  11. Structure and cytotoxic activity of sesquiterpene glycoside esters from Calendula officinalis L.: Studies on the conformation of viridiflorol.

    Science.gov (United States)

    D'Ambrosio, Michele; Ciocarlan, Alexandru; Colombo, Elisa; Guerriero, Antonio; Pizza, Cosimo; Sangiovanni, Enrico; Dell'Agli, Mario

    2015-09-01

    Topic applications of Calendula officinalis L. lipophilic extracts are used in phytotherapy to relieve skin inflammatory conditions whereas infusions are used as a remedy for gastric complaints. Such a different usage might be explained by some cytotoxicity of lipophilic extracts at gastric level but little is known about this. Therefore, we screened the CH2Cl2 extract from the flowers of C. officinalis by MTT and LDH assays in human epithelial gastric cells AGS. This bioassay-oriented approach led to the isolation of several sesquiterpene glycosides which were structurally characterized by spectroscopic measurements, chemical reactions and MM calculations. The conformational preferences of viridiflorol fucoside were established and a previously assigned stereochemistry was revised. The compounds 1a, 2a and 3f showed comparably high cytotoxicity in the MTT assays, whereas the effect on LDH release was lower. Our study provides new insights on the composition of C. officinalis extracts of medium polarity and identifies the main compounds that could be responsible for cytotoxic effects at gastric level.

  12. Total flavonoid content in varieties of Calendula officinalis L. originating from different countries and cultivated in Estonia.

    Science.gov (United States)

    Raal, Ain; Kirsipuu, Kadri

    2011-03-01

    Total flavonoid contents in the inflorescences of different varieties of Calendula officinalis L. were investigated. The commercial seeds (20 samples) of varieties of C. officinalis originating from eight European countries were cultivated in home gardens in two different counties of Estonia. Total flavonoid contents, determined spectrophotometrically (λ = 370 nm), varied from each other by more than three times (0.21-0.68%) in the investigated varieties. The variety with the highest flavonoid content was 'Kablouna', produced by the Finnish company Siemen (0.68%). Other varieties with high flavonoid content, such as 'Touch of Red' produced by the Latvian company Kurzemes Seklas (0.55%), 'Golden Emperor' produced by the Finnish company Suvipiha (0.50%), 'Pomyk' from Poland (0.50%), etc., may also be preferred for cultivation as natural sources, as they are also rich in flavonoids. The amount of total flavonoids depends on the variety and/or the place and time of cultivation. There appeared to be no conclusive relationship between the total flavonoid content and the colour of ligulate and tubular florets of C. officinalis.

  13. D-Glucose as a modifying agent in gelatin/collagen matrix and reservoir nanoparticles for Calendula officinalis delivery.

    Science.gov (United States)

    Lam, P-L; Kok, S H-L; Bian, Z-X; Lam, K-H; Tang, J C-O; Lee, K K-H; Gambari, R; Chui, C-H

    2014-05-01

    Gelatin/Collagen-based matrix and reservoir nanoparticles require crosslinkers to stabilize the formed nanosuspensions, considering that physical instability is the main challenge of nanoparticulate systems. The use of crosslinkers improves the physical integrity of nanoformulations under the-host environment. Aldehyde-based fixatives, such as formaldehyde and glutaraldehyde, have been widely applied to the crosslinking process of polymeric nanoparticles. However, their potential toxicity towards human beings has been demonstrated in many previous studies. In order to tackle this problem, D-glucose was used during nanoparticle formation to stabilize the gelatin/collagen-based matrix wall and reservoir wall for the deliveries of Calendula officinalis powder and oil, respectively. In addition, therapeutic selectivity between malignant and normal cells could be observed. The C. officinalis powder loaded nanoparticles significantly strengthened the anti-cancer effect towards human breast adenocarcinoma MCF7 cells and human hepatoma SKHep1 cells when compared with the free powder. On the contrary, the nanoparticles did not show significant cytotoxicity towards normal esophageal epithelial NE3 cells and human skin keratinocyte HaCaT cells. On the basis of these evidences, D-glucose modified gelatin/collagen matrix nanoparticles containing C. officinalis powder might be proposed as a safer alternative vehicle for anti-cancer treatments.

  14. Antibacterial activity of antipsoriatic herbs: Cassia tora, Momordica charantia and Calendula officinalis

    Directory of Open Access Journals (Sweden)

    T S Roopashree

    2008-09-01

    Full Text Available Summary: In view of increasing resistance to existing antimicrobial agents, herbal drugs are being looked as very importance source for discovery of new agents for treating various ailments related to bacterial infections. Cassia tora, Calendula officinalis and Momordica charantia are well known plants in Asia including India which posses wide range of pharmacological activities. These drugs have been used in India as  folk remedy in the form of decoctions and infusions to treat bacterial infections and also claimed to be an effective against variety of skin conditions like psoriasis, acne, wounds etc. The present investigation was carried out to study the unexplored area of these drugs towards their antibacterial activity with respect to their traditional use as antipsoriatic agents. The herbs were subjected to successive extraction using different solvents and the extracts were subjected to antibacterial evaluation against both gram positive and gram negative organisms by cup plate technique. Among the various extracts, aqueous extracts were found to be more effective against all the bacteria. Staphyllococcus aureus was more susceptible to the aqueous extracts among the tested organisms.   Industrial relevance: The selected herbs have been used traditionally for treating skin diseases like psoriasis, for which there is no complete cure till date. Though exact mechanism of these herbs for their effectiveness in psoriasis is not understood, according to American academy of dermatology antibacterial therapy could also be used for treating psoriasis. In view of this study of antibacterial activity was carried out. Among the various extracts, aqueous extracts exhibited highest activity especially against S. aureus which has been one of the organisms which aggravate conditions like psoriasis. As the aqueous extracts have shown excellent activity, these extracts could be formulated individually or in combination as external or internal dosage forms

  15. Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis Utilização de técnicas de cultura de tecidos na proliferação de brotos e enraizamento de Calendula officinalis

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    Cristiane Pimentel Victório

    2012-09-01

    Full Text Available The high demand for plant material from Calendula officinalis in the production of herbal medicines and cosmetics, turns the technique of plant-tissue culture into one of the alternatives for the improvement of crops over a short period of time. A protocol for tissue culture was developed from segments of seedlings of C. officinalis, in order to improve the proliferation of shoots and roots. We used a Murashige and Skoog (MS½N medium, reduced to half the concentrations of NH4NO3 and KNO3 to verify the effect of different types of explants (basal, intermediate, and apical, a medium containing beach sand as support instead of agar, and the effect of auxins and cytokinins (TDZ tidiazuron; BAP, 6-benzylaminopurine, IAA, indol-3-acetic acid, IBA, indol-3-butyric acid, NAA, naphthalene-acetic acid on plant development in vitro. The results showed pronounced rooting from the apical explants, as well as a greater elongation of shoots and number of leaves. The solid medium was more suitable for the C. officinalis cultures. Shoot proliferation was dependent on cytokinins with better results from the influence of TDZ or BAP compared to the other treatments. Plants regenerated from the medium containing TDZ displayed a glazed appearance and morphogenetic deformations. The highest rate for rooting (80% was obtained using IAA 0.1mgL-1. Through in-vitro propagation, healthy C. officinalis plants were obtained with roots which can acclimatise, allowing the continuous supplement of raw material.A alta demanda por material vegetal de Calendula officinalis para produção de fitoterápicos e cosméticos, configura a técnica de cultura de tecidos vegetais como uma das alternativas para o aprimoramento das culturas em curto período de tempo. Um protocolo de cultura de tecidos foi desenvolvido a partir de segmentos de plântulas de C. officinalis, no intuito de melhorar a proliferação de brotos e o enraizamento. Foi utilizado o meio Murashige e Skoog (MS

  16. Antioxidant activity of Calendula officinalis extract: inhibitory effects on chemiluminescence of human neutrophil bursts and electron paramagnetic resonance spectroscopy.

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    Braga, Pier Carlo; Dal Sasso, Monica; Culici, Maria; Spallino, Alessandra; Falchi, Mario; Bertelli, Aldo; Morelli, Roberto; Lo Scalzo, Roberto

    2009-01-01

    There is growing interest in natural chemical compounds from aromatic, spicy, medicinal and other plants with antioxidant properties in order to find new sources of compounds inactivating free radicals generated by metabolic pathways within body tissue and cells, mainly polymorphonuclear leukocytes (PMNs) whose overregulated recruitment and activation generate a large amount of reactive oxygen species (ROS) and reactive nitrogen species (RNS), leading to an imbalance of redox homeostasis and oxidative stress. The aim of this study was to examine whether a propylene glycol extract of Calendula officinalis interferes with ROS and RNS during the PMN respiratory bursts, and to establish the lowest concentration at which it still exerts antioxidant activity by means of luminol-amplified chemiluminescence. Electron paramagnetic resonance (EPR) spectroscopy was also used in order to confirm the activity of the C. officinalis extract. The C. officinalis extract exerted its anti-ROS and anti-RNS activity in a concentration-dependent manner, with significant effects being observed at even very low concentrations: 0.20 microg/ml without L-arginine, 0.10 microg/ml when L-arginine was added to the test with phorbol 12-myristate 13-acetate and 0.05 microg/ml when it was added to the test with N-formyl-methionyl-leucyl-phenylalanine. The EPR study confirmed these findings, 0.20 microg/ml being the lowest concentration of C. officinalis extract that significantly reduced 2,2-diphenyl-1-picrylhydrazyl. These findings are interesting for improving the antioxidant network and restoring the redox balance in human cells with plant-derived molecules as well as extending the possibility of antagonizing the oxidative stress generated in living organisms when the balance is in favor of free radicals as a result of the depletion of cell antioxidants.

  17. Pharmacodynamic Research and Safety Evaluation of Calendula Officinalis L.%金盏菊的安全性评价及药效学研究

    Institute of Scientific and Technical Information of China (English)

    郑佳; 卢先明; 徐利; 于宗强

    2013-01-01

    Objective: To study the pharmacodynamic of Calendula officinalis L and to evaluate its safety. Methods: The safety evaluation of Calendula officinalis L was evaluated by maximum tolerance of mice. The effects of Calendula officinali L. on bleeding time and blood coagulation time in mice were observed through the methods of tail cutting and blood coagulation in glass capillary and anti - inflammatory effect was investigated by xylene - induced mice ear swelling test Results: The maximum tolerance dose to mice was 80 g/kg, which was equal to 320 times of aducts'maximum oral dose per day. The bleeding time was shortened significantly by the high dosage of Calendula officinalis L decocting; the blood coagulation time was also shortened significantly by high dosage, middle dosage and low dosage of Calendula officinalis L decocting, and Calendula officinalis L decocting has certain trend on anti - inflammatory effect, but it did not have statistical significance. Conclusion: There was no any acute toxic reaction of Calendula officinalis L decocting and the security is very well It has the effect of hemostasis and has a certain anti - inflammatory effect The renewable resources of Calendula officinalis L are rich and it is worthy to be developed and utilized.%目的:对金盏菊的药效学进行初步研究,并对其安全性做出评价.方法:采用最大耐受量测定法对金盏菊进行安全性评价;分别采用剪尾法、毛细玻璃管法、二甲苯致小鼠耳肿胀法观察金盏菊对小鼠出血时间、凝血时间的影响及抗炎作用.结果:小鼠最大耐受量为80g/kg,为成人每日最大用量的320倍.金盏菊水煎液高剂量组能明显缩短小鼠的出血时间(P<0.05);金盏菊水煎液高剂量组、中剂量组、低剂量组均能明显缩短小鼠的凝血时间(P<0.05);金盏菊水煎液对二甲苯致小鼠耳肿胀有一定的抗炎趋势,但不具备统计学意义.结论:金盏菊水煎液安全性好,有良好的止

  18. In vitro acaricidal activity of ethanolic and aqueous floral extracts of Calendula officinalis against synthetic pyrethroid resistant Rhipicephalus (Boophilus) microplus.

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    Godara, R; Katoch, R; Yadav, A; Ahanger, R R; Bhutyal, A D S; Verma, P K; Katoch, M; Dutta, S; Nisa, F; Singh, N K

    2015-09-01

    Detection of resistance levels against deltamethrin and cypermethrin in Rhipicephalus (Boophilus) microplus collected from Jammu (India) was carried out using larval packet test (LPT). The results showed the presence of resistance level II and I against deltamethrin and cypermethrin, respectively. Adult immersion test (AIT) and LPT were used to evaluate the in vitro efficacy of ethanolic and aqueous floral extracts of Calendula officinalis against synthetic pyrethroid resistant adults and larvae of R. (B.) microplus. Four concentrations (1.25, 2.5, 5 and 10 %) of each extract with four replications for each concentration were used in both the bioassays. A concentration dependent mortality was observed and it was more marked with ethanolic extract. In AIT, the LC50 values for ethanolic and aqueous extracts were calculated as 9.9 and 12.9 %, respectively. The egg weight of the live ticks treated with different concentrations of the ethanolic and aqueous extracts was significantly lower than that of control ticks; consequently, the reproductive index and the percent inhibition of oviposition values of the treated ticks were reduced. The complete inhibition of hatching was recorded at 10 % of ethanolic extract. The 10 % extracts caused 100 % mortality of larvae after 24 h. In LPT, the LC50 values for ethanolic and aqueous extracts were determined to be 2.6 and 3.2 %, respectively. It can be concluded that the ethanolic extract of C. officinalis had better acaricidal properties against adults and larvae of R. (B.) microplus than the aqueous extract.

  19. Evaluation of in vitro anticancer activity of Ocimum basilicum, Alhagi maurorum, Calendula officinalis and their parasite Cuscuta campestris.

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    Mandana Behbahani

    Full Text Available The present investigation was carried out to study the relationship between presence of cytotoxic compounds in Ocimum basilicum, Alhagi maurorum, Calendula officinalis and their parasite Cuscuta campestris. The cytotoxic activity of the pure compounds was performed by MTT assay against breast cancer cell lines (MCF-7 and MDA-MB-231 and normal breast cell line (MCF 10A. The induction of apoptosis was measured by the expression levels of p53, bcl-2, bax and caspase-3 genes using quantitative Real Time PCR. Three active fractions were detected by nuclear magnetic resonance as lutein, lupeol and eugenol, respectively, in C. officinalis, A. maurorum and O. basilicum. These compounds and their epoxidized forms were also detected in their parasite C. campestris. The cytotoxic activity of lutein epoxide, lupeol epoxide and eugenol epoxide was significantly more than lutein, lupeol and eugenol. The mRNA expression level of p53, caspase-3 and bax genes were increased in both cancer cells treated with all pure compounds. However, bcl-2 gene expression decreased in treated breast cancer cells. In conclusion, all the data indicated that the epoxide forms of lupeol, lutein and eugenol are potential drug candidates for inducing apoptosis in human breast cancer cells.

  20. Evaluation of in vitro anticancer activity of Ocimum basilicum, Alhagi maurorum, Calendula officinalis and their parasite Cuscuta campestris.

    Science.gov (United States)

    Behbahani, Mandana

    2014-01-01

    The present investigation was carried out to study the relationship between presence of cytotoxic compounds in Ocimum basilicum, Alhagi maurorum, Calendula officinalis and their parasite Cuscuta campestris. The cytotoxic activity of the pure compounds was performed by MTT assay against breast cancer cell lines (MCF-7 and MDA-MB-231) and normal breast cell line (MCF 10A). The induction of apoptosis was measured by the expression levels of p53, bcl-2, bax and caspase-3 genes using quantitative Real Time PCR. Three active fractions were detected by nuclear magnetic resonance as lutein, lupeol and eugenol, respectively, in C. officinalis, A. maurorum and O. basilicum. These compounds and their epoxidized forms were also detected in their parasite C. campestris. The cytotoxic activity of lutein epoxide, lupeol epoxide and eugenol epoxide was significantly more than lutein, lupeol and eugenol. The mRNA expression level of p53, caspase-3 and bax genes were increased in both cancer cells treated with all pure compounds. However, bcl-2 gene expression decreased in treated breast cancer cells. In conclusion, all the data indicated that the epoxide forms of lupeol, lutein and eugenol are potential drug candidates for inducing apoptosis in human breast cancer cells.

  1. Mutagenic influences of colchicine on phenological and molecular diversity of Calendula officinalis L.

    Science.gov (United States)

    El-Nashar, Y I; Ammar, M H

    2016-04-26

    Six different colchicine concentrations: 0, 400, 800, 1200, 1600, and 2000 ppm, in combination with four soaking time treatments (1, 2, 3, and 4 h), were selected to assess the effects on germination, vegetative growth, and flower yield components in calendula plants. The molecular diversity among the treatments was assessed using ten SRAP marker combinations. Seed soaking in colchicine significantly enhanced both the fresh and the dry shoot and root masses, flowering date, number of flowers per plant, and flower diameter. At 1200-ppm colchicine combined with a 4-h soaking time, a superior effect on seed germination was observed, whereas 800 ppm for 4 h produced the highest number of flowers and the largest flower diameter. The earliest flowering time was found at 800 ppm combined with a short soaking time (1 h), while the 4-h soaking time with 800 ppm, is recommended for growing calendula outdoors, since it enhances flower development. At the molecular level, 752 fragments were successfully amplified using the SRAP primers, with 280 genetic loci found throughout the calendula genome. The polymorphism percentage ranged from 79 to 100% and the polymorphic information content (PIC) values ranged between 0.85 and 0.97. The high number of detected loci and PIC values suggests a great power of SRAP markers in detecting mutant molecular diversity. Our results clearly show the existence of genetic variation among colchicine treated calendula plants and the clustering of the studied mutants was concordant with the colchicine concentration used.

  2. Assessment of plant development, morphology and flavonoid content in different cultivation treatments of Calendula officinalis L: , Asteraceae

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    Luis Fernando Nunes Alves Paim

    2010-12-01

    Full Text Available The objective of this study was to examine the influence of cultivation treatments on plant development, morphology and flavonoid content in Calendula officinalis L., Asteraceae. The cultivation treatments were liming (LIM, liming + organic fertilizer (LORG and liming + NPK chemical fertilizer (LCHEM. The experiment was completely randomized with sixty repetitions and the means were compared by ANOVA and Duncan's test (significance of 95%. The results show that LORG and LCHEM produced greater plant development compared with LIM treatment. The LORG treatment also produced plants with greater homogeneity, fresh mass, and higher numbers of flowers per plant, but lower biomass of flower produced by each individual. Morphoanatomical variations were also observed with tubular flowers with yellow centres (TCA and tubular flowers with brown centres (TAC that produced a higher content of flavonoids (1.41 and 1.44% respectively, compared with ligulate flowers with yellow centres (LCA and ligulate flowers with brown centres (LAC (0.89 and 0.95% respectively. The chemical compounds in the flowers of calendula viewed by thin-layer chromatography showed that the treatments did not have any influence on the qualitative chemical profile of the plant. However, cultivars of calendula that underwent treatment LCHEM produced a greater quantity of total flavonoids (1.14% against 1.04% for LORG and 1.03% for LIM.O objetivo deste estudo foi avaliar a influência dos tratos culturais no desenvolvimento vegetal, morfologia e teor de flavonóides para Calendula officinalis L., Asteraceae. Os tratos culturais foram: calagem (CA, calagem + adubação orgânica (CAO e calagem + adubação química NPK (CAQ. O experimento foi inteiramente casualizado com sessenta repetições e a comparação das médias realizada por ANOVA e teste de Duncan (significância de 95%. Os resultados apontam que CAO e CAQ apresentaram maior desenvolvimento vegetal comparados com tratamento CA. O

  3. Qualitative and quantitative high performance thin layer chromatography analysis of Calendula officinalis using high resolution plate imaging and artificial neural network data modelling.

    Science.gov (United States)

    Agatonovic-Kustrin, S; Loescher, Christine M

    2013-10-10

    Calendula officinalis, commonly known Marigold, has been traditionally used for its anti-inflammatory effects. The aim of this study was to investigate the capacity of an artificial neural network (ANN) to analyse thin layer chromatography (TLC) chromatograms as fingerprint patterns for quantitative estimation of chlorogenic acid, caffeic acid and rutin in Calendula plant extracts. By applying samples with different weight ratios of marker compounds to the system, a database of chromatograms was constructed. A hundred and one signal intensities in each of the HPTLC chromatograms were correlated to the amounts of applied chlorogenic acid, caffeic acid, and rutin using an ANN. The developed ANN correlation was used to quantify the amounts of 3 marker compounds in calendula plant extracts. The minimum quantifiable level (MQL) of 610, 190 and 940 ng and the limit of detection (LD) of 183, 57 and 282 ng were established for chlorogenic, caffeic acid and rutin, respectively. A novel method for quality control of herbal products, based on HPTLC separation, high resolution digital plate imaging and ANN data analysis has been developed. The proposed method can be adopted for routine evaluation of the phytochemical variability in calendula extracts.

  4. The comparison of in vivo antigenotoxic and antioxidative capacity of two propylene glycol extracts of Calendula officinalis (marigold) and vitamin E in young growing pigs.

    Science.gov (United States)

    Frankic, T; Salobir, K; Salobir, J

    2009-12-01

    The objective of the study was to evaluate the protective effect of Calendula officinalis propylene glycol extracts against oxidative DNA damage and lipid peroxidation induced by high polyunsaturated fatty acid (PUFA) intake in young growing pigs. Forty young growing pigs were assigned to five treatment groups: control; oil (linseed oil supplementation); C. officinalis 1 and 2 groups (linseed oil plus 3 ml/day of C. officinalis propylene glycol extracts); and vitamin E group (linseed oil plus 100 mg/kg of vitamin E). Lymphocyte DNA fragmentation and 24-h urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) excretion were measured to determine DNA damage. Lipid peroxidation was studied by analysing plasma and urine malondialdehyde (MDA), and urine isoprostane concentrations (iPF2α-VI), total antioxidant status of plasma and glutathione peroxidase (GPx) assays. C. officinalis 1 (extract from petals) effectively protected DNA from oxidative damage. It indicated a numerical trend towards the reduction of plasma MDA and urinary iPF2α-VI excretion. Its effect was comparable with that of vitamin E. C. officinalis 2 (extract from flower tops) showed less antioxidant potential than the extract from petals. We can conclude that the amount of C. officinalis extracts proposed for internal use by traditional medicine protects the organism against DNA damage induced by high PUFA intake.

  5. The effect of different nitrogen fertilization rates on yield and quality of marigold (Calendula officinalis L. 'Tokaj' raw material

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    Beata Król

    2012-12-01

    Full Text Available Pot marigold (Calendula officinalis L. is an annual ornamental plant which is also grown for herbal raw material (flower heads used in the pharmaceutical and cosmetic industries. A field experiment was carried out in the years 2006-2008 in the Experimental Farm of the University of Life Sciences in Lublin. The study was conducted on loess soil with the granulometric composition of silt loam. The aim of the experiment was to determine the effect of different nitrogen rates (0, 40, 80, 120, 160 kg N × ha-1 on some morphological features of flower heads as well as on yield and quality of pot marigold raw material. Flowering of pot marigold was shortest in the control treatment (32 days and longest (43 days in the plot where nitrogen fertilization had been applied at the highest rate (160 kg N × ha-1. Nitrogen fertilization had a significant influence on the number of flower heads per plant, but no significant difference was found in diameter as well as in ligulate flowers and tubular flowers in the flower head. It was found to increase significantly raw material yield after the application of 80 kg N × ha-1, compared to the control treatment. Yield of flower heads did not differ markedly for fertilization rates from 80 to 160 kg N × ha-1. Nitrogen fertilization modified slightly essential oil content (this content increased with increasing nitrogen rates, but at the same time it decreased the percentage of flavonoid compounds.

  6. Effect of sowing date and plant density on grain and flower yield of Pot Marigold (Calendula officinalis L.

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    mohamad javad seghatol eslami

    2009-06-01

    Full Text Available Pot marigold (Calendula officinalis L. is a medicinal herb whose dried flower heads are used to heal wounds. In order to study the effects of sowing dates and plant density on grain and flower yield of pot marigold, an experiment was conducted at Agricultural Research Center of Islamic Azad University, Birjand Branch in 2005. Three sowing dates (30 March, 14 April and 30 April and three plant densities (plant distances on row were 10, 20 and 30 centimeters were compared in a split- plot experiment based on a randomized complete block design with 3 replications. Seed and flower yields were significantly different at planting dates and plant densities. Sowing date had significant effects on flower and seed harvest index. The latest sowing dates had the highest flower and seed harvest index. Plant density had not significant effect on flower harvest index, but the effect on seed harvest index, was significant. In total our result showed that the first sowing date with 25 plants/m2 had the highest grain and flower yield. Keywords: Marigold, sowing date, plant density, medicinal plant.

  7. Lead content in pot marigold (Calendula officinalis L.) inflorescences and leaves: impact of precipitations and vicinity of motorway.

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    Meos, Andres; Jüriado, Tiiu; Matto, Vallo; Raal, Ain

    2011-05-01

    Trace metal contamination is a major environmental and health problem virtually in all countries. The present study was aimed to estimate the lead content of pot marigold (Calendula officinalis L.) inflorescences and leaves collected from a nonpolluted test field. The lead content in dry pot marigold inflorescences was 9.34 ± 0.79 µg/g, in dry leaves 11.57 ± 0.47 µg/g, and in soil 0.649 ± 0.012 µg/g. The distance of pot marigold collection beds (30-220 m from the motorway) had no effect on lead content. There was a strong positive correlation between the amount of precipitations and lead content of pot marigold leaves but not inflorescences indicating the soil as primarily the source of increased lead content. In conclusion, no effect of motorway vicinity was found for pot marigold inflorescences or leaves lead content; however, as a precaution, it is not recommended to collect the plants during or just after showers.

  8. Anti-inflammatory, anti-tumor-promoting, and cytotoxic activities of constituents of marigold (Calendula officinalis) flowers.

    Science.gov (United States)

    Ukiya, Motohiko; Akihisa, Toshihiro; Yasukawa, Ken; Tokuda, Harukuni; Suzuki, Takashi; Kimura, Yumiko

    2006-12-01

    Ten oleanane-type triterpene glycosides, 1-10, including four new compounds, calendulaglycoside A 6'-O-methyl ester (2), calendulaglycoside A 6'-O-n-butyl ester (3), calendulaglycoside B 6'-O-n-butyl ester (5), and calendulaglycoside C 6'-O-n-butyl ester (8), along with five known flavonol glycosides, 11-15, were isolated from the flowers of marigold (Calendula officinalis). Upon evaluation of compounds 1-9 for inhibitory activity against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation (1 microg/ear) in mice, all of the compounds, except for 1, exhibited marked anti-inflammatory activity, with ID50 values of 0.05-0.20 mg per ear. In addition, when 1-15 were evaluated against the Epstein-Barr virus early antigen (EBV-EA) activation induced by TPA, compounds 1-10 exhibited moderate inhibitory effects (IC50 values of 471-487 mol ratio/32 pmol TPA). Furthermore, upon evaluation of the cytotoxic activity against human cancer cell lines in vitro in the NCI Developmental Therapeutics Program, two triterpene glycosides, 9 and 10, exhibited their most potent cytotoxic effects against colon cancer, leukemia, and melanoma cells.

  9. Effect of Arbuscular Mycorrhizal Fungi On Yield and Phytoremediation Performance of Pot Marigold (Calendula officinalis L.) Under Heavy Metals Stress.

    Science.gov (United States)

    Tabrizi, Leila; Mohammadi, Siavash; Delshad, Mojtaba; Moteshare Zadeh, Babak

    2015-01-01

    In order to study the effect of mycorrhizal fungi (inoculated and non-inoculated) and heavy metals stress [0, Pb (150 and 300 mg/kg) and Cd (40 and 80 mg/kg)] on pot marigold (Calendula officinalis L.), a factorial experiment was conducted based on a randomized complete block design with 4 replications in Research Greenhouse of Department of Horticultural Sciences, University of Tehran, Iran, during 2012-2013. Plant height, herbal and flower fresh and dry weight, root fresh and dry weight and root volume, colonization percentage, total petal extract, total petal flavonoids, root and shoot P and K uptakes, and Pb and Cd accumulations in root and shoot were measured. Results indicated that with increasing soil Pb and Cd concentration, growth and yield of pot marigold was reduced significantly; Cd had greater negative impacts than Pb. However, mycorrhizal fungi alleviated these impacts by improving plant growth and yield. Pot marigold concentrated high amounts of Pb and especially Cd in its roots and shoots; mycorrhizal plants had a greater accumulation of these metals, so that those under 80 mg/kg Cd soil(-1) accumulated 833.3 and 1585.8 mg Cd in their shoots and roots, respectively. In conclusion, mycorrhizal fungi can improve not only growth and yield of pot marigold in heavy metal stressed condition, but also phytoremediation performance by increasing heavy metals accumulation in the plant organs.

  10. An investigation of influence of solvent on the degradation kinetics of carotenoids in oil extracts of Calendula officinalis

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    DEJAN BEZBRADICA

    2005-02-01

    Full Text Available The stability of carotenoids was studied in marigold oil extracts prepared with following solvents: Myritol 312®, paraffin oil, almond oil, olive oil, sunflower oil, grape seed oil, and soybean oil. The concentration of the carotenoids was determined by spectroscopic measurement at 450 nm. Degradation rate showed a first order dependence on the concentration of carotenoids with a faster first stage (which lasted 35–50 days, depending on the solvent and a slower second stage. The highest degradation rates were observed in extracts prepared with linoleic acid rich solvents (sunflower oil, soybean oil and grape seed oil, while the lowest were found in oil with saturated fatty acids (Myritol 312® and paraffin oil. These results confirm the connection between the degradation of carotenoids and lipid autoxidation, and suggest that the influence of the oil solvents on the stability of oil extracts of Calendula officinalis is a factor that must be considered when selecting a solvent for the production of marigold oil extracts.

  11. A Bio-Guided Fractionation to Assess the Inhibitory Activity of Calendula officinalis L. on the NF-κB Driven Transcription in Human Gastric Epithelial Cells

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    Elisa Colombo

    2015-01-01

    Full Text Available Calendula officinalis L. has been largely known for its topical anti-inflammatory properties; however, there are no experimental evidences about its antiphlogistic effect at the gastric level. To investigate whether marigold might exert an activity against gastric inflammation, a CH2Cl2 extract obtained from C. officinalis flowers was evaluated in vitro on the NF-κB pathway. The lipophilic extract demonstrated a significant inhibitory effect on the NF-κB driven transcription. The identification of active compounds was conducted by a bio-guided fractionation of the extract that afforded 16 fractions. Fraction J exhibited a concentration-dependent inhibitory activity on the NF-κB driven transcription and significantly contributed to the antiphlogistic effect showed by CH2Cl2 extract. The main components of fraction J were loliolide and the fucoside acetates of β-eudesmol and viridiflorol. HPLC analysis of fractions D and E led to the identification and isolation of triterpene esters that showed a concentration-dependent inhibition of the NF-κB driven transcription, with faradiol-3-myristate and the corresponding aglycone being the most active compounds. The present study provides some experimental evidences that Calendula officinalis L. may exert an anti-inflammatory activity on the gastric district by the inhibition of the NF-κB system, identifying the compounds responsible, at least in part, for the observed effect.

  12. Antimicrobial activity of Calendula officinalis, Camellia sinensis and chlorhexidine against the adherence of microorganisms to sutures after extraction of unerupted third molars

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    Raquel Lourdes Faria

    2011-10-01

    Full Text Available OBJECTIVE: The objective of this study was to compare the antimicrobial effect of mouthwashes containing Calendula officinalis L., Camellia sinensis (L. Kuntze and 0.12% chlorhexidine digluconate on the adherence of microorganisms to suture materials after extraction of unerupted third molars. MATERIAL AND METHODS: Eighteen patients with unerupted maxillary third molars indicated for extraction were selected (n=6 per mouthwash. First, the patients were subjected to extraction of the left tooth and instructed not to use any type of antiseptic solution at the site of surgery (control group. After 15 days, the right tooth was extracted and the patients were instructed to use the Calendula officinalis, Camellia sinensis or chlorhexidine mouthwash during 1 week (experimental group. For each surgery, the sutures were removed on postoperative day 7 and placed in sterile phosphate-buffered saline. Next, serial dilutions were prepared and seeded onto different culture media for the growth of the following microorganisms: blood agar for total microorganism growth; Mitis Salivarius bacitracin sucrose agar for mutans group streptococci; mannitol agar for Staphylococcus spp.; MacConkey agar for enterobacteria and Pseudomonas spp., and Sabouraud dextrose agar containing chloramphenicol for Candida spp. The plates were incubated during 24-48 h at 37ºC for microorganism count (CFU/mL. RESULTS: The three mouthwashes tested reduced the number of microorganisms adhered to the sutures compared to the control group. However, significant differences between the control and experimental groups were only observed for the mouthwash containing 0.12% chlorhexidine digluconate. CONCLUSIONS: Calendula officinalis L. and Camellia sinensis (L. Kuntze presented antimicrobial activity against the adherence of microorganisms to sutures but were not as efficient as chlorhexidine digluconate.

  13. COMPARATIVE ANALYSIS OF THE USE OF CMR POMADE (BELLISPERENIS + CALENDULA OFFICINALIS + MYRISTICASEBIFERA AND PHYSIOLOGICAL SOLUTION IN TISSUE SCARRING OF RATS (RATHUSNORVEGICUS

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    E. S. Fernandes

    2014-09-01

    Full Text Available Pharmaceutical market has several types of ointments that are used in animals for healing processes, arising from traumatic injuries or surgical procedures. The use of homeopathic medicines in the treatment of skin wounds has grown significantly, but no one knows for sure the efficiency of such drugs. Thus, this paper aimed to measure the drug's effectiveness CMR (Bellisperenis + Calendula officinalis Myristica sebifera in comparison to physiologic solution in treating skin wounds produced in rats. Standardized in the back of the animals on both sides (right and left evaluating the healing potential of homeopathic ointment CMR compared to saline lesions were performed.

  14. Treatment of Bothrops alternatus envenomation by Curcuma longa and Calendula officinalis extracts and ar-turmerone Tratamento local do envenenamento por Bothrops alternatus com extrato de Curcuma longa e Calendula officinalis e ar-turmerone

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    M.M. Melo

    2005-02-01

    Full Text Available It was investigated the efficiency of two extracts of plants and one fraction of their properties against the local effects of bothropic envenomation. Bothrops alternatus venom (1.25µg diluted in 100µl of sterile saline solution was inoculated (intradermally into the shaved dorsal back skin of 30 New Zealand rabbits. The animals were divided in six groups receiving the following treatments: group I: subcutaneous application of Curcuma longa extract (1.0ml; group II: topic treatment of Curcuma longa hydroalcoholic extract (1.0ml; group III: topic application of ar-turmerone in vaseline (1.0g; group IV: topic application of Curcuma longa methanolic extract (1.0ml; group V: topic application of Calendula officinalis ointment (1.0g; group VI: topic application of saline (1.0ml. These treatments were done at 30 minutes, and at 2, 4, 24 and 72 hours after venom inoculation. Intensity of local edema, hemorrhagic halo and necrosis were evaluated until 168h after that. Additionally, seven days after the Bothrops venom inoculation, blood was collected from heart with and without EDTA (10% for hemogram and biochemical parameters (total protein, blood urea nitrogen, creatinine, and fibrinogen and all the animals were anesthetized, sacrificed by ether inhalation and submitted to necropsy. Fragments of tissues were taken for histopathological evaluation. The most efficient treatment for inhibition of edema, necrosis and local hemorrhage after Bothrops alternatus venom was the topic application of ar-turmerone.Investigou-se a eficácia do extrato de plantas no tratamento local do envenenamento botrópico. Veneno de serpentes Bothrops alternatus (1,25µg diluído em 100µl de solução salina estéril foi inoculado (via intradérmica entre as escápulas de 30 coelhos. Os animais foram divididos em seis grupos (tratamentos: grupo I: tratamento subcutâneo com extrato de Curcuma longa; grupo II: tratamento tópico com extrato hidroalcoólico de Curcuma longa

  15. High performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC) for the qualitative and quantitative analysis of Calendula officinalis-advantages and limitations.

    Science.gov (United States)

    Loescher, Christine M; Morton, David W; Razic, Slavica; Agatonovic-Kustrin, Snezana

    2014-09-01

    Chromatography techniques such as HPTLC and HPLC are commonly used to produce a chemical fingerprint of a plant to allow identification and quantify the main constituents within the plant. The aims of this study were to compare HPTLC and HPLC, for qualitative and quantitative analysis of the major constituents of Calendula officinalis and to investigate the effect of different extraction techniques on the C. officinalis extract composition from different parts of the plant. The results found HPTLC to be effective for qualitative analysis, however, HPLC was found to be more accurate for quantitative analysis. A combination of the two methods may be useful in a quality control setting as it would allow rapid qualitative analysis of herbal material while maintaining accurate quantification of extract composition.

  16. Planting depth for oilseed calendula

    Science.gov (United States)

    Calendula (Calendula officinalis L.) is not only a popular ornamental plant in temperate climates, but also a potential oilseed crop. Its seed oil has high levels of calendic acid, which makes it a highly valued drying oil with important industrial applications. Much basic agronomic information on c...

  17. Protective effect of Calendula officinalis Linn. flowers against 3-nitropropionic acid induced experimental Huntington's disease in rats.

    Science.gov (United States)

    Shivasharan, B D; Nagakannan, Pandian; Thippeswamy, Boreddy Shivanandappa; Veerapur, Veeresh Prabakar; Bansal, Punit; Unnikrishnan, Mazhuvancherry K

    2013-10-01

    Oxidative stress (OS) and nitric oxide mechanisms have been recently proposed in 3-nitropropionic acid (3-NP)-induced neurotoxicity. The compounds, having antioxidant, anti-inflammatory and estrogenic effects, have been suggested for neuroprotection in different experimental models. Calendula officinalis Linn. flower extract (COE) is known for its potent antioxidant, anti-inflammatory, estrogenic and neuroprotective activities. Hence, the present study was designed to evaluate the neuroprotective effect of COE on 3-NP-induced neurotoxicity in rats by observing behavioral changes, OS and striatal damage in rat brain. Adult female Wistar rats were pretreated with vehicle or COE (100 and 200 mg/kg) for 7 days, followed by cotreatment with 3-NP (15 mg/kg, intraperitoneally) for the next 7 days. At the end of the treatment schedule, rats were evaluated for alterations in sensory motor functions and short-term memory. Animals were sacrificed and brain homogenates were used for the estimation of lipid peroxidation (LPO), glutathione, total thiols, glutathione S-transferase, catalase and nitrite. A set of brain slices was used for the evaluation of neuronal damage in the striatal region of the brain. 3-NP caused significant alterations in animal behavior, oxidative defense system evidenced by raised levels of LPO and nitrite concentration, and depletion of antioxidant levels. It also produced a loss of neuronal cells in the striatal region. Treatment with COE significantly attenuated behavioral alterations, oxidative damage and striatal neuronal loss in 3-NP-treated animals. The present study shows that COE is protective against 3-NP-induced neurotoxicity in rats. The antioxidant, anti-inflammatory and estrogenic properties of COE may be responsible for its neuroprotective action.

  18. EFFECTS OF PLANTING SPACE AND HARVEST TIME ON THE NUMBER, WEIGHT AND DIAMETER OF MARIGOLD (CALENDULA OFFICINALIS L. FLOWERS

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    Nada Parađiković

    2013-06-01

    Full Text Available The study was conducted during 2010 in marigold (Calendula officinalis L. to determine the effects of three plant densities (plant density A - 65 cm x 35 cm; plant density B - 65 cm x 25 cm; plant density C – 55 cm x 25 cm and harvest time on the number, weight and diameter of marigold flowers. The results showed that the plant density significantly influenced the number of flowers per plant and flower weight. The largest number of flowers per plant was recorded in the plant density B (13.2 and the lowest (9.87 in the plant density C. The lowest flower weight was recorded in the plant density C (1.31 g and was statistically lower than the flower weight in the plant densities A (1.42 g and B (1.38 g. The plant density significantly influenced the number of flowers on side branches, being the highest in the plant density B. The diameter of the marigold flower was not significantly influenced by the plant density. During the experiment, a total of 13 harvests were achieved. The greatest number of flowers per plant was harvested in the eighth, ninth and tenth harvest, while the largest flower weight was measured in the fifth and twelfth harvest. On the average, the number of flowers per plant / harvest was 11.63 and the weight of flowers was 1.38 g. Diameter of marigold flowers ranged from 2.89 cm to 3.59 cm in the thirteenth and the third harvest, respectively. The number of flowers on side branches per plant / harvest was 11.61.

  19. Development of lamellar gel phase emulsion containing marigold oil (Calendula officinalis) as a potential modern wound dressing.

    Science.gov (United States)

    Okuma, C H; Andrade, T A M; Caetano, G F; Finci, L I; Maciel, N R; Topan, J F; Cefali, L C; Polizello, A C M; Carlo, T; Rogerio, A P; Spadaro, A C C; Isaac, V L B; Frade, M A C; Rocha-Filho, P A

    2015-04-25

    Appropriate therapeutics for wound treatments can be achieved by studying the pathophysiology of tissue repair. Here we develop formulations of lamellar gel phase (LGP) emulsions containing marigold (Calendula officinalis) oil, evaluating their stability and activity on experimental wound healing in rats. LGP emulsions were developed and evaluated based on a phase ternary diagram to select the best LGP emulsion, having a good amount of anisotropic structure and stability. The selected LGP formulation was analyzed according to the intrinsic and accelerated physical stability at different temperatures. In addition, in vitro and in vivo studies were carried out on wound healing rats as a model. The LGP emulsion (15.0% marigold oil; 10.0% of blend surfactants and 75.0% of purified water [w/w/w]) demonstrated good stability and high viscosity, suggesting longer contact of the formulation with the wound. No cytotoxic activity (50-1000 μg/mL) was observed in marigold oil. In the wound healing rat model, the LGP (15 mg/mL) showed an increase in the leukocyte recruitment to the wound at least on days 2 and 7, but reduced leukocyte recruitment after 14 and 21 days, as compared to the control. Additionally, collagen production was reduced in the LGP emulsion on days 2 and 7 and further accelerated the process of re-epithelialization of the wound itself. The methodology utilized in the present study has produced a potentially useful formulation for a stable LGP emulsion-containing marigold, which was able to improve the wound healing process.

  20. Optimization of chemometric approaches for the extraction of isorhamnetin-3-O-rutinoside from Calendula officinalis L.

    Science.gov (United States)

    Moraes, Maria Lourdes Leite; da Silva, Heron Dominguez Torres; Blanes, Lucas; Doble, Philip; Tavares, Marina Franco Maggi

    2016-06-05

    The application of Design of Experiments (DoE) to the determination of optimum conditions for an extraction process relies on the correct selection of mathematical models. The linear model is the one typically used; however, in some cases it does not always have superior performance, ignoring the real nature of the data and its appropriate descriptive model. In order to evaluate the extraction efficiency of isorhamnetin-3-O-rutinoside from flowers of Calendula officinalis L. a multivariate factorial analysis was used. Simulations were conducted using linear, quadratic, full cubic and special cubic models. A Simplex-Centroid design was chosen as it delivered greater precision with only minor errors versus other models tested. Analyses were performed by capillary zone electrophoresis using sodium tetraborate buffer (40mmolL(-1), pH 9.4) containing 10% methanol. The detection was linear over a range of 8.0-50.0mgL(-1) (r(2)=0.996), and the limits of detection (LOD) and quantification (LOQ) for isorhamnetin-3-O-rutinoside were 3.44mgL(-1) and 11.47mgL(-1), respectively. The full cubic model showed the best extraction results, with an error of 3.40% compared to analysis of variance, and a determination coefficient of 0.974. The difference between the responses at the reference point, calculated by the model, and the experimental response, varies around 2.72% for full cubic model. Comparison of the four models showed the full cubic model was the most appropriate one, allowing greater efficiency in the extraction of isorhamnetin-3-O-rutinoside. Selection of the model made it possible to obtain a 60% increase in sensitivity compared to the linear model.

  1. Reviews of Salicylic Acid Mist Spray on Amount of Flavonoids, Anthocyanins, Sugar and Root Performance at Marigold Flower Species (Calendula Officinalis L at Hydroponics System

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    Fatemeh Naghavi

    2016-03-01

    Full Text Available This research is performed in order of studying anti-stress effect of salicylic acid material on Calendula Officinalis L after germinating. Different Concentrations of salicylic acid ( 2,4,+ and 8 milimolar on Seedlings of Calendula Officinalis L flower Hydroponic and greenhouse conditions during 4 days was studied. Between concentration of mist sprayed salicylic acid, 6 molar concentration had the best effect on Qualitative and quantitative performance of mentioned plant. Reviews indicate that high concntrations cause stop in growth of plant and Destruction of plant and also low concentrations had no particular effect. Results from measuring Flavonoids, anthocyanins and root performance showed that their rate after 24 hours in comparison to Control plants, indicates a meaningful increase. On treating salicylic acid there was a meaningful decrease on Germination of treated plants in comparison to control group was observed. Overally, salicylic acid at 6 molar concentration results in increase of Flavonoids, root, anthocyanins and Soluble sugars in comparison to control group.

  2. 不同培养基质对金盏菊生长的影响研究%Effects of Different Culture Medium on the Growth of Calendula officinalis

    Institute of Scientific and Technical Information of China (English)

    陈峰; 沈迎春; 刘阳; 李莉

    2014-01-01

    Using different culture medium,using pot Calendula officinalis as experimental material,research the effect of garden soil,mushroom residue,vermiculite and mixed of these three medium as culture medi-um on the growth and development of Calendula officinalis. The test results show that:the different culture medium containing nutrients difference;effect of different culture medium on the growth of Calendula offici-nalis is different;effect of different culture substrates on chlorophyll content,Catalase activity,superoxide dis-mutase(SOD)activity are different. Comprehensive morphological and physiological indexes on the growth of Calendula officinalis can be concluded:the mushroom residue as growth culture medium of Calendula offici-nalis is best.%采用不同的培养基质,利用盆栽金盏菊作为试验材料,研究田园土、菌渣、蛭石及三者的混合基质作为培养基对金盏菊生长发育的影响。结果表明:不同培养基质所含营养成分差别较大;不同培养基质对金盏菊生长发育影响不同;不同培养基质对金盏菊叶绿素含量、过氧化氢酶活性、超氧化物歧化酶(SOD)活性等影响均不同。综合金盏菊生长的形态指标和生理指标可知:用菌渣作为培养基质的金盏菊长势最好。

  3. The Water Fraction of Calendula officinalis Hydroethanol Extract Stimulates In Vitro and In Vivo Proliferation of Dermal Fibroblasts in Wound Healing.

    Science.gov (United States)

    Dinda, Manikarna; Mazumdar, Swagata; Das, Saurabh; Ganguly, Durba; Dasgupta, Uma B; Dutta, Ananya; Jana, Kuladip; Karmakar, Parimal

    2016-10-01

    The active fraction and/or compounds of Calendula officinalis responsible for wound healing are not known yet. In this work we studied the molecular target of C. officinalis hydroethanol extract (CEE) and its active fraction (water fraction of hydroethanol extract, WCEE) on primary human dermal fibroblasts (HDF). In vivo, CEE or WCEE were topically applied on excisional wounds of BALB/c mice and the rate of wound contraction and immunohistological studies were carried out. We found that CEE and only its WCEE significantly stimulated the proliferation as well as the migration of HDF cells. Also they up-regulate the expression of connective tissue growth factor (CTGF) and α-smooth muscle actin (α-SMA) in vitro. In vivo, CEE or WCEE treated mice groups showed faster wound healing and increased expression of CTGF and α-SMA compared to placebo control group. The increased expression of both the proteins during granulation phase of wound repair demonstrated the potential role of C. officinalis in wound healing. In addition, HPLC-ESI MS analysis of the active water fraction revealed the presence of two major compounds, rutin and quercetin-3-O-glucoside. Thus, our results showed that C. officinalis potentiated wound healing by stimulating the expression of CTGF and α-SMA and further we identified active compounds. Copyright © 2016 John Wiley & Sons, Ltd.

  4. Antioxidant Capacity of Calendula Officinalis Flowers Extract and Prevention of Radiation Induced Oropharyngeal Mucositis in Patients with Head and Neck Cancers: A Randomized Controlled Clinical Study

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    Mohammad Taghi Kazemi

    2013-03-01

    Full Text Available This study was designed to determine the effect of Calendula officinalis flowers extract mouthwash as oral gel on radiation-induced oropharyngeal mucositis (OM in patients with head-and-neck cancer. Forty patients with neck and head cancers under radiotherapy or concurrent chemoradiotherapy protocols were randomly assigned to receive either 2% calendula extract mouthwash or placebo (20 patients in each group. Patients were treated with telecobalt radiotherapy at conventional fractionation (200 cGy/fraction, five fractions weekly, 30–35 fractions within 4–7 weeks. The oropharyngeal mucositis was evaluated by two clinical investigators (a radiation oncologist and a dentist, using the oral mucositis assessment scale (OMAS. Trying to find out the possible mechanism of action of the treatment, total antioxidant, polyphenol and flavonoid contents, and quercetin concentration of the mouth wash were measured. Calendula mouthwash significantly decreased the intensity of OM compared to placebo at week 2 (score: 5.5 vs. 6.8, p = 0.019, week 3 (score: 8.25 vs. 10.95, p < 0.0001 and week 6 (score: 11.4 vs. 13.35, p = 0.031. Total antioxidant, polyphenol and flavonoid contents and quercetin concentration of the 2% extract were 2353.4 ± 56.5 μM, 313.40 ± 6.52 mg/g, 76.66 ± 23.24 mg/g, and 19.41 ± 4.34 mg/l, respectively. Calendula extract gel could be effective on decreasing the intensity of radiotherapy- induced OM during the treatment and antioxidant capacity may be partly responsible for the effect.

  5. Hepato and reno protective action of Calendula officinalis L. flower extract.

    Science.gov (United States)

    Preethi, Korengath Chandran; Kuttan, Ramadasan

    2009-03-01

    Flower extract of C. officinalis L. was evaluated for its protective effect against CCl4 induced acute hepatotoxicity and cisplatin induced nephrotoxicity. The activities of serum marker enzymes of liver injury like glutamate pyruvate transaminase (SGPT), glutamate oxaloacetate transaminase (SGOT) and alkaline phosphatase (ALP) which were increased by CCl4 injection was found to be significantly reduced by the pretreatment of the flower extract at 100 and 250 mg/kg body weight. The lipid peroxidation in liver, the marker of membrane damage and the total bilirubin content in serum were also found to be at significantly low level in the extract pretreated group, indicating its protective role. The kidney function markers like urea and creatinine were significantly increased in cisplatin treated animals. However, their levels were found to be lowered in the extract pretreated groups (100 and 250 mg/kg body weight). Moreover, cisplatin induced myelosuppression was ameliorated by the extract pretreatment. Treatment with the extract produced enhancement of antioxidant enzymes--superoxide dismutase and catalase and glutathione. Results suggest a protective role of the flower extract of C. officinalis against CCl4 induced acute hepatotoxicity and cisplatin induced nephrotoxicity. Extract has been found to contain several carotenoids of which lutein, zeaxanthin and lycopene predominates. Possible mechanism of action of the flower extract may be due to its antioxidant activity and reduction of oxygen radicals.

  6. Analysis of the essential oils from Calendula officinalis growing in Brazil using three different extraction procedures Análise por CG-EM do óleo essencial de Calendula officinalis cultivado no Brasil utilizando-se três diferentes processos de extração

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    Zilda Cristina Gazim

    2008-09-01

    Full Text Available Terpenes and aroma volatiles from flowers of Calendula officinalis cultivated in southeastern Brazil were obtained by steam distillation (SD, headspace-cold finger (HS-CF extraction and headspace solid-phase microextraction (HS-SPME coupled with gas chromatography and mass spectrometric analysis. The dried flowers contained 0.1% oil. Kovats indices and mass spectra were used to identify 27 individual components in the various volatile fractions. The main components present in the volatile fractions of the C. officinalis flowers, obtained by SD, HS-SPME, and HS-CF, were δ-cadinene (22.5, 22.1, and 18.4 % and γ-cadinene (8.9, 25.4, and 24.9 % while 20.4 % of α-cadinol was seen only after SD extraction.Terpenos e aromas voláteis das flores de Calendula officinalis cultivados no sudoeste do Brasil foram isolados por arraste a vapor (SD, dedo frio (HS-CF e micro extração em fase sólida (HS-SPME acoplada à espectrometria de massas. As flores secas da C. officinalis contêm 0,1% de óleo essencial e foram identificadas 27 substâncias químicas através do cálculo do índice de Kováts e interpretação dos espectros de massas. As substâncias majoritárias presentes no óleo essencial das flores de C. officinalis, obtido por SD, HS-SPME e HS-CF foram δ-cadinene (22,5; 22,1 e 18,4 % γ-cadinene (8,9, 25,4 e 24,9 % e 20.4 % de α-cadinol foi observado apenas na extração por arraste a vapor.

  7. A new extract of the plant calendula officinalis produces a dual in vitro effect: cytotoxic anti-tumor activity and lymphocyte activation

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    Collado Antonia

    2006-05-01

    Full Text Available Abstract Background Phytopharmacological studies of different Calendula extracts have shown anti-inflamatory, anti-viral and anti-genotoxic properties of therapeutic interest. In this study, we evaluated the in vitro cytotoxic anti-tumor and immunomodulatory activities and in vivo anti-tumor effect of Laser Activated Calendula Extract (LACE, a novel extract of the plant Calendula Officinalis (Asteraceae. Methods An aqueous extract of Calendula Officinalis was obtained by a novel extraction method in order to measure its anti-tumor and immunomodulatory activities in vitro. Tumor cell lines derived from leukemias, melanomas, fibrosarcomas and cancers of breast, prostate, cervix, lung, pancreas and colorectal were used and tumor cell proliferation in vitro was measured by BrdU incorporation and viable cell count. Effect of LACE on human peripheral blood lymphocyte (PBL proliferation in vitro was also analyzed. Studies of cell cycle and apoptosis were performed in LACE-treated cells. In vivo anti-tumor activity was evaluated in nude mice bearing subcutaneously human Ando-2 melanoma cells. Results The LACE extract showed a potent in vitro inhibition of tumor cell proliferation when tested on a wide variety of human and murine tumor cell lines. The inhibition ranged from 70 to 100%. Mechanisms of inhibition were identified as cell cycle arrest in G0/G1 phase and Caspase-3-induced apoptosis. Interestingly, the same extract showed an opposite effect when tested on PBLs and NKL cell line, in which in vitro induction of proliferation and activation of these cells was observed. The intraperitoneal injection or oral administration of LACE extract in nude mice inhibits in vivo tumor growth of Ando-2 melanoma cells and prolongs the survival day of the mice. Conclusion These results indicate that LACE aqueous extract has two complementary activities in vitro with potential anti-tumor therapeutic effect: cytotoxic tumor cell activity and lymphocyte activation

  8. Total antioxidant and oxidant status of plasma and renal tissue of cisplatin-induced nephrotoxic rats: protection by floral extracts of Calendula officinalis Linn.

    Science.gov (United States)

    Verma, Pawan Kumar; Raina, Rajinder; Sultana, Mudasir; Singh, Maninder; Kumar, Pawan

    2016-01-01

    The present study was aimed to determine the total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) of plasma and renal tissue in cisplatin (cDDP) induced nephrotoxic rats and its protection by treatments with floral extracts of Calendula officinalis Linn. Treatment with cDDP elevated (p GSH) and antioxidant enzymes compared to the control group. Pre- and post-treatments of ethanolic floral extract of C. officinalis along with cDDP restored (p > 0.05) CR, albumin, TOS, GSH and activities of antioxidant enzymes in blood and renal tissue. Ethanolic extract treatments reduced (p < 0.05) MDA level in renal tissue without restoring the erythrocyte MDA level following cDDP treatment. These observations were further supported by the histopathological findings in renal tissue. Observations of the present study have shown that treatments with ethanolic floral extract of C. officinalis protect cDDP induced nephrotoxicity by restoring antioxidant system of the renal tissue.

  9. Produção de biomassa de calendula officinalis l. adubada com fósforo e cama-de-frango Biomass yield of Calendula officinalis L. fertilized with phosporus and chicken manure

    Directory of Open Access Journals (Sweden)

    Valdenise Carbonari Barboza

    2009-04-01

    Full Text Available Objetivou-se, neste trabalho, avaliar a influência do fósforo e da cama-de-frango semidecomposta na produção de capítulos florais da Calendula officinalis L. Os fatores em estudo foram cinco doses de fósforo (4,3; 25,8; 43,0; 60,2; 81,7 kg ha-1 de P2O5, na forma de superfosfato triplo e cinco doses de cama-de-frango semidecomposta (1.000; 6.000; 10.000; 14.000 e 19.000 kg ha-1. Para combinar o fósforo e a cama-de-frango, usou-se a matriz experimental Plan Puebla III resultando em nove tratamentos (25,8 e 6.000; 60,2 e 6.000; 25,8 e 14.000; 60,2 e 14.000; 43,0 e 10.000; 4,3 e 6.000; 81,7 e 14.000; 25,8 e 1.000; 60,2 e 19.000 kg ha-1 de P e cama-de-frango, respectivamente no delineamento de blocos casualizados, com quatro repetições. A altura média final das plantas, aos 120 dias, ficou em torno de 60,0 cm, independente dos tratamentos. A produção máxima de capítulos florais da calêndula foi de 9.755,16 mil ha-1,obtida com o uso de 49,15 kg ha-1 de P2O5 associada a 13.401,10 kg ha-1 de cama-de-frango. A maior produção (11.058,89 kg ha-1de massa fresca de capítulos florais de calêndula foi obtida com 49,98 kg ha-1 de P2O5 e 13.266,59 kg ha-1 de cama-de-frango. As produções máximas de massas secas de capítulos florais (2.227,61 kg ha-1 foram obtidas com 39,62 kg ha-1 de P2O5 e 8.109,75 kg ha-1 de cama-de-frango. Concluiu-se que para se obter maior produção de massa seca de capítulos florais, componente de maior importância comercial, a calêndula deve ser cultivada utilizando adubação com HH 40 kg ha-1 de P2O5 e H" 8.000 kg ha-1 de cama-de-frango.The aim of this work was to evaluate the influence of phosphorus and semi-decomposed chicken manure on capitula yield of Calendula officinalis L. The studied factors were five doses of phosphorus (4.3, 25.8, 43.0, 60.2, and 81.7 kg ha-1 P2O5 in the triple super phosphate form and five doses of semi-decomposed chicken manure (1000, 6000, 10000, 14000, and 19000 kg ha-1. The Plan

  10. EFFECT OF POLYPHENOLIC COMPOUNDS ISOLATED FROM CARTHAMUS TINCTORIUS AND CALENDULA OFFICINALIS L., ON FUNCTIONAL ACTIVITY OF IMMUNE CELLS UNDER CONDITIONS OF CYTOSTATIC IMMUNOSUPPRESSION

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    N. V. Masnaya

    2013-01-01

    Full Text Available The purpose of the study – to study the effect of polyphenolic compounds extracted from the flowers of safflower oil and calendula, the functional activity of immune cells in cytotoxic immune suppression.Conventional methods determined the total number of splenocytes, relative (% and absolute (106, the number of antibody-forming cells (AFC in the spleen of mice by local hemolysis by Cunningham.Evaluated the effect of compounds of natural origin on the cellular immune response in the delayed-type hypersensitivity (DTH. Phagocytic activity of peritoneal macrophages was determined by the method based on the intensity of their capture ink particles. We studied the functional activity of peritoneal macrophages by NBT test (spontaneous and stimulated. Studies were conducted on male mice Category 1 (conventional linear mouse line CBA/CaLac aged 2–2.5 months, weighing 20–22 g. After the introduction mice line CBA/CaLac of polyphenolic compounds derived from flowers of Carthamus tinctorius and flowers of Calendula officinalis L. during the 5-day course in dose 50 mg/kg was observed stimulation of the humoral immune response (total number of splenocytes, the number of antibodies in the spleen cells and the functional activity of macrophages and Immunomodulating effect on the humoral immunity and the functional activity of macrophages after a single injection of cyclophosphamide in dose 250 mg/kg. Immunotropic activity of polyphenolic compounds is higher than that those of the reference product of tincture of Echinacea purpurea.

  11. Cicatrização de úlceras por pressão com extrato Plenusdermax® de Calendula officinalis L.

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    Marcelo Buzzi

    2016-04-01

    Full Text Available RESUMO Objetivo: Avaliar os benefícios terapêuticos do extrato de bioativos de Calendula officinalis Plenusdermax® na cicatrização de úlceras de pressão. Métodos: estudo observacional de coorte realizado com quarenta e um pacientes com diagnóstico de úlcera por pressão com tamanho da ferida estável por mais de três meses. Os pacientes foram avaliados quinzenalmente durante 30 semanas, em relação a redução da área da lesão, controle de infecção, tipos de tecido e exsudato e microbiologia das úlceras. Resultados: a proporção de pacientes que cicatrizaram completamente após 15 e 30 semanas de tratamento foi 63% e 88%, respectivamente, sendo que a média de tempo de cicatrização foi de 12,5 ± 7,8 semanas. Não foram observados eventos adversos durante o tratamento. Conclusão: os resultados do estudo indicam que Plenusdermax® de bioativos de C. officinalis é um tratamento seguro que promove a cicatrização de úlceras de pressão.

  12. Post-emergence herbicides useful in calendula

    Science.gov (United States)

    Easy and effective weed control is required by growers who are considering new industrial crops. Post-emergence herbicides typically are the products of choice by today’s growers. Unfortunately, post-emergence herbicides with proven safety margins are not known for calendula (Calendula officinalis),...

  13. Calendula and camelina response to nitrogen fertility

    Science.gov (United States)

    The emerging oil-seed crops calendula (Calendula officinalis) and camelina (Camelina sativa L.) can provide a domestic, renewable, non-food alternative to imported oil sources for bioenergy and industrial purposes. However, very little information exists concerning nitrogen (N) fertilizer guidelines...

  14. Eficiência nutricional do Nitrogênio e produção de biomassa em Calendula officinalis L. (Asteraceae em condições de casa de vegetação Nitrogen nutritional efficiency and biomass production in Calendula officinalis L. (Asteraceae under greenhouse conditions

    Directory of Open Access Journals (Sweden)

    A.P. Serra

    2013-01-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito da aplicação de diferentes doses de nitrogênio no solo e de épocas de coleta sobre a eficiência nutricional do nitrogênio e a produção de biomassa em Calendula officinalis L. (Asteraceae em condições de casa de vegetação. O substrato utilizado foi a camada de 0 - 20 cm de Latossolo Vermelho Distroférrico. O delineamento experimental foi inteiramente casualizado, com quatro repetições. Os tratamentos foram constituídos de quatro doses de N (0, 21, 42 e 84mg dm-3 e cinco épocas de colheita (15, 30, 45, 60 e 90 dias após o transplante. O teor de N na inflorescência de calêndula reduziu com as épocas de coleta, redução essa que ocorreu possivelmente devido à menor eficiência da absorção desse elemento pela planta e menor translocação do N para as inflorescências. Pode se concluir que as épocas de avaliação influenciaram na resposta da planta à eficiência de absorção (EFA, sendo a máxima EFA de N aos 51 dias após o transplante das mudas. Observou-se aumento na translocação de N da parte aérea (folhas e caules para as inflorescências, tendo como consequência a redução do N na parte aérea. Houve incremento na produção de biomassa de calêndula em função das épocas de coleta , e do aumento das doses de N aplicadas no solo. A eficiência nutricional do N foi influenciada pelas épocas de avaliação e pelo teor de N presente no solo.The aim of this study was to evaluate the effect of both the application of different nitrogen levels on the soil and the harvest periods on nitrogen nutritional efficiency and biomass production in Calendula officinalis L. (Asteraceae under greenhouse conditions. The employed substrate was 0-20cm layer of Oxisol. Experimental design was completely randomized, with four replicates. Treatments consisted of four N levels (0, 21, 42 and 84 mg dm-3 and five harvest periods (15, 30, 45, 60 and 90 days after transplanting. N levels in

  15. Effects of Planting Date and Plant Density on Physiological Indices, Quantity and Quality Traits of Two Varieties of Marigold (Calendula officinalis L.

    Directory of Open Access Journals (Sweden)

    A Sepehri

    2016-07-01

    Full Text Available Introduction Marigold (Calendula officinalis L. is originated from North West Africa and Mediterranean area, is a medicinal plant used for several purposes. It is an annual herb or short-lived perennial from the Asteraceae family with yellow or orange flowers. The Marigold has been used as a traditional medicine and food dye, but is currently used as an anti-inflammatory and wound healer. It is grown for drug, obtained from the flowers. The flowers blossom during summer three or more times per year. The essential oil of yellow or orange petals of Calendula officinalis L. is one of the important yield components which is used for food and medicine. Moreover, the seed has an oil content of 5-20 %. Seed oil could be used as a binder in paints, coating and cosmetics. Growth, development and production of medicinal plants, as well as other plants are affected by genetic and agronomic factors. Planting date and plant density are two most important factors that can affect yield and yield components. Planting date affects the quantity and quality of secondary metabolites of medicinal plants. The optimum sowing date and plant density can improve the light and temperature absorption and other factors during the growing season. The positive effects of optimal planting date and plant density has been described by a number of researchers. The Plant population is dependent on the plant characters, growth period, time and method of cultivation. Also, the suitable sowing date has advantages for maximum production. Early sowing in the spring causes weakly establishment of plant and late planting date shortens growth period and simultaneous flowering period due to high temperature in summer. In this study, the effects of plant density and planting date on physiological indices, quantity and quality of two varieties of spare and compact marigold has been evaluated. Materials and Methods In order to determine the effects of planting date and plant density on

  16. A Prospective, Descriptive Study to Assess the Clinical Benefits of Using Calendula officinalis Hydroglycolic Extract for the Topical Treatment of Diabetic Foot Ulcers.

    Science.gov (United States)

    Buzzi, Marcelo; de Freitas, Franciele; Winter, Marcos

    2016-03-01

    Diabetic foot ulcers (DFUs) have a significant impact on patient quality of life. A prospective, descriptive pilot study was conducted between May 2012 and December 2013 through the dermatology outpatient unit in a Brazilian hospital to evaluate the clinical benefits of using Calendula officinalis hydroglycolic extract in the treatment of DFUs. Patients diagnosed with a stable neuropathic ulcer of >3 months' duration; ranging in size from 0.5-40 cm(2); without osteomyelitis, gangrene, bone exposure, cancer, or deep tissue infection; ages 18-90 years; with adequate glycemic control and no history of an allergy to C. officinalis were enrolled. Patients provided demographic and diabetes-related information and were evaluated biweekly for 30 weeks or until healing (ie, full epithelialization with no wound drainage). DFUs were measured and clinically examined for microbiological flora and presence of odor, tissue type (eg, granulation, fibrin sloth, necrosis), exudate, and retraction rate using planimetry images. Patients' blood tests and neuropathic pain assessment (the latter by clinician-directed questionnaire) were performed at baseline and the end of treatment; pain also was assessed during dressing changes using a 10-point rating scale. Patients' ulcers were treated twice daily with C. officinalis hydroglycolic extract spray solution and covered with saline-moistened, sterile, nonadherent gauze and bandages followed by foot offloading with adequate protective footwear. Patients received their first treatment in the clinic then performed care at home. From a potential population of 109 patients, 25 did not meet the inclusion criteria. Of the remaining 84 participants enrolled, 43 withdrew before study completion; cited reasons included lost to follow-up (16), medical judgment (2), failure to attend >3 scheduled visits (17), protocol violation (5), and death (3). Forty-one (41) - 17 women, average age 62 years (range 44-82 years), average glycemic level 153 mg

  17. Potencial de extratos à base de Calendula officinalis L. na indução da síntese de fitoalexinas e no efeito fungistático sobre Botrytis cinerea, in vitro Potential of Calendula officinalis L. extracts in inducing phytoalexin synthesis and fungistatic effect on Botrytis cinerea in vitro

    Directory of Open Access Journals (Sweden)

    S.M. Mazaro

    2013-01-01

    Full Text Available Foram desenvolvidos três experimentos com o objetivo de avaliar o potencial de preparados a base de calêndula (Calendula officinalis L. na indução de fitoalexinas em cotilédones de soja, na indução de mecanismos de resistência em frutos de morango, e o efeito fungistático sobre Botrytis cinerea in vitro. O delineamento experimental foi inteiramente casualizado com quatro repetições, para 15 tratamentos resultantes da combinação de três formas de extração (extrato alcoólico, infusão, e maceração em cinco concentrações (zero; 1,25; 2,5; 5; e 10%. Os resultados demonstram que os preparados de C. officinalis apresentaram capacidade de indução das fitoalexinas gliceolinas em cotilédones de soja. Na aplicação dos preparados em pós-colheita de morangos ocorreu alteração no teor de flavonóides, bem como a atividade da enzima FAL foi estimulada pela aplicação dos extratos; no entanto, não foi constatado o controle de podridão dos frutos. O efeito fungistático foi observado na extração por maceração em todas as suas concentrações reduzindo o crescimento do fungo B. cinerea in vitro sendo que, a partir de 2,5%, observou-se inibição total. A extração por infusão também apresentou resposta positiva na redução do crescimento de B. cinerea, com melhor resposta na concentração de 10% do preparado.Three experiments were carried out to evaluate the potential of calendula (Calendula officinalis L. extracts for phytoalexin induction in soybean cotyledons, resistance mechanism induction in strawberry fruits and fungistatic effect on Botrytis cinerea in vitro. Experimental design was completely randomized with 15 treatments resulting from the combination of three forms of extraction (alcohol extract, infusion and maceration at five concentrations (zero, 1.25, 2.5, 5 and 10%. Results showed that C. officinalis extracts could induce the phytoalexins glyceollins in soybean cotyledons. In the application of extracts

  18. 金盏花药学研究概况%Overview of Pharmacological Research on Calendula officinalis L.

    Institute of Scientific and Technical Information of China (English)

    孙曼; 赵兵; 姚默; 李祥楠; 廖雁; 巩江; 倪士峰

    2011-01-01

    Based on extensive literature search, this paper summarized species, composition, pharmacology, clinical application of C. Officinalis , to provide scientific materials for further development and utilization.%在广泛文献检索基础上,对金盏花的种属、成分、药理、临床应用等进行了概述,为其深入开发利用提供了科学资料.

  19. Teor e rendimento de flavonóides em calêndula (Calendula officinalis L. cultivada com diferentes lâminas de irrigação Flavonoid content and yield in marigold plant (Calendula officinalis L. cultivated under different irrigation depths

    Directory of Open Access Journals (Sweden)

    D.P.G. Bortolo

    2009-01-01

    Full Text Available A Calendula officinalis L. apresenta uso ornamental, culinário e terapêutico. A inflorescência é usada com ação cicatrizante e antiinflamatória. O experimento analisou o efeito de quatro lâminas de irrigação em condições de campo, na produção de flavonóides em calêndula. As lâminas baseadas na evaporação do Tanque Classe A (ECA foram: 0, 50, 100 e 150% ECA; sendo aplicadas desde o início do florescimento até o final do ciclo da cultura. O teor de flavonóides não apresentou diferenças estatísticas. Os maiores valores de matéria seca de flores e rendimento de flavonóides foram obtidos no tratamento sem irrigação. O teor de prolina foi maior para o tratamento com estresse por excesso hídrico. A deficiência hídrica acumulada para o tratamento sem irrigação sinalizou uma possível morte por deficiência hídrica. Com a ocorrência da precipitação no período de pré-florescimento, a planta, originária do Mediterrâneo, respondeu com florescimento abundante em massa e em número de flores. Assim, para Presidente Prudente, caso não ocorra a reposição hídrica do solo por precipitação, é necessário o uso de irrigação complementar com 200 mm apenas no período de pré-florescimento, para induzir ao máximo florescimento e máximo rendimento de flavonóides por hectare.Calendula officinalis L. presents ornamental, culinary and therapeutic use. Its inflorescence is used to heal and as an anti-inflammatory. This study analyzed the effect of four different irrigation depths under field conditions on flavonoid production in marigold plant (Calendula officinalis L.. The water depths were estimated based on the class A pan evaporation (CPE: 0, 50, 100 and 150% CPE; they were applied from the beginning of flowering to the end of the culture cycle. There were no statistical differences in flavonoid content. The highest values of flower dry weight and flavonoid yield were obtained with the treatment without irrigation

  20. Repellency Effects of Essential Oils of Myrtle (Myrtus Communis, Marigold (Calendula Officinalis Compared with DEET Against Anopheles Stephensi on Human Volunteers

    Directory of Open Access Journals (Sweden)

    M Khoobdel

    2011-12-01

    Full Text Available Background: Malaria and leishmaniasis are two most significant parasitic diseases which are endemic in Iran. Over the past decades, interest in botanical repellents has increased as a result of safety to human. The comparative effi­cacy of essential oils of two native plants, myrtle (Myrtus communis and marigold (Calendula officinalis collected from natural habitats at southern Iran was compared with DEET as synthetic repellent against Anopheles stephensi on human subjects under laboratory condition. Methods: Essential oils from two species of native plants were obtained by Clevenger-type water distillation. The protec­tion time of DEET, marigold and myrtle was assessed on human subject using screened cage method against An. stephensi. The effective dose of 50% essential oils of two latter species and DEET were determined by modified ASTM method. ED50 and ED90 values and related statistical parameters were calculated by probit analysis. Results: The protection time of 50% essential oils of marigold and myrtle were respectively 2.15 and 4.36 hours com­pared to 6.23 hours for DEET 25%. The median effective dose (ED50 of 50% essential oils was 0.1105 and 0.6034 mg/cm2 respectively in myrtle and marigold. The figure for DEET was 0.0023 mg/cm2.Conclusion: This study exhibited that the repellency of both botanical repellents was generally lower than DEET as a synthetic repellent. However the 50% essential oil of myrtle showed a moderate repellency effects compared to mari­gold against An. stephensi.

  1. 卢旺达产金盏菊花化学成分研究Ⅱ%Study on the constituents from Rwandan Calendula officinalis flos Ⅱ

    Institute of Scientific and Technical Information of China (English)

    陈玥; 李晓霞; 武一玫; 董勇喆; 王涛; 张祎

    2014-01-01

    目的 对卢旺达产金盏菊(Calendula officinalisL)的化学成分进行研究,为进一步开发利用该植物资源提供依据.方法 采用大孔吸附树脂、葡聚糖凝胶Sephadex LH-20等柱色谱及制备型高效液相色谱等方法进行分离纯化,并通过理化性质与波谱学数据相结合的方法鉴定化合物的结构.结果 从金盏菊中分离鉴定了6个单体成分,分别为金盏菊苷A(1,calendnoside A)、异鼠李素(2,isorhamnetin)、异鼠李素3-O-β-D-吡喃葡萄糖苷(3,isorhamnetin 3-O-β-D-glucopyranoside)、异鼠李素3-O-(6"-乙酰基)-β-D-吡喃葡萄糖苷(4,isorhamnetin 3-O-(6"-acetyl)-β-D-glucopyranoside)、异鼠李素3-O-β-D-呋喃洋芹糖基(1→2)-β-D-吡喃葡萄糖苷(5,isorhamnetin 3-O-β-D-apiofurranosyl(1→2)-β-D-glucopyranoside)和isorhamnetin 3-O-(2-O-β-xylopyranosyl-6-O-β-rhamnopyranosyl)-β-glucopyranoside (6).结论 化合物1为新化合物,化合物4~6为首次从金盏菊属植物中分离得到.

  2. Repellency Effects of Essential Oils of Myrtle (Myrtus communis, Marigold (Calendula officinalis Compared with DEET against Anopheles stephensi on Human Volunteers

    Directory of Open Access Journals (Sweden)

    M Tavassoli

    2011-12-01

    Full Text Available Background: Malaria and leishmaniasis are two most significant parasitic diseases which are endemic in Iran. Over the past decades, interest in botanical repellents has increased as a result of safety to human. The comparative effi­cacy of essential oils of two native plants, myrtle (Myrtus communis and marigold (Calendula officinalis collected from natural habitats at southern Iran was compared with DEET as synthetic repellent against Anopheles stephensi on human subjects under laboratory condition. Methods:  Essential oils from two species of native plants were obtained by Clevenger-type water distillation. The protec­tion time of DEET, marigold and myrtle was assessed on human subject using screened cage method against An. stephensi. The effective dose of 50% essential oils of two latter species and DEET were determined by modified ASTM method. ED50 and ED90 values and related statistical parameters were calculated by probit analysis.   Results: The protection time of 50% essential oils of marigold and myrtle were respectively 2.15 and 4.36 hours com­pared to 6.23 hours for DEET 25%. The median effective dose (ED50 of 50% essential oils was 0.1105 and 0.6034 mg/cm2 respectively in myrtle and marigold. The figure for DEET was 0.0023 mg/cm2. Conclusion: This study exhibited that the repellency of both botanical repellents was generally lower than DEET as a synthetic repellent. However the 50% essential oil of myrtle showed a moderate repellency effects compared to mari­gold against An. stephensi.

  3. Calendula: A Potential New Oilseed Crop for the Northern U.S.

    Science.gov (United States)

    Seeds of calendula (Calendula officinalis) are a rich source of calendic acid (conjugated C18:3), a highly oxidative fatty acid that can be used to replace volatile organic compounds (VOC) as a drying agent in many industrial chemicals including paints and adhesives. Calendula flourishes in temperat...

  4. Influência do substrato e do tamanho da célula de bandejas de poliestireno expandido no desenvolvimento de mudas e produção de calêndula (Calendula officinalis L. Influence of substrate and cell size of expanded polystyrene tray on the development and production of marigold (Calendula officinalis L. seedlings

    Directory of Open Access Journals (Sweden)

    C.K.R Barbosa

    2010-03-01

    Full Text Available A calêndula (Calendula officinalis L. é uma planta medicinal anual de origem Mediterrânica que apresenta propriedades antiinflamatórias, antivirais, antigenotóxicas. A propagação desta planta normalmente é feita por sementes, sendo o uso de bandejas de poliestireno uma forma econômica de produção de mudas. Foram conduzidos dois experimentos com o objetivo de avaliar a interferência de dois tamanhos de célula em bandeja de poliestireno expandido (40 cm³ e 12 cm³ e de dois substratos (solo + esterco bovino curtido e substrato comercial tipo Plantmax para hortaliças® sobre a qualidade de mudas e a produção de calêndula. O delineamento estatístico nos dois experimentos foi em blocos casualizados, dispostos em arranjo fatorial 2 x 2 (dois substratos e dois tamanhos de células com seis repetições. Os tratamentos, referentes aos tamanhos das células foram bandeja com 128 células (40 cm³ de volume e, bandeja com 288 células (12 cm³ de volume. Os substratos utilizados foram solo de cerrado + esterco bovino curtido (1:1 e substrato comercial tipo "Plantmax para hortaliças®". Parte das mudas foi transplantada para avaliação da produção de capítulos. Houve diferenças significativas entre os substratos para as variáveis: matéria seca de parte aérea, matéria seca da raiz e matéria seca total. O uso do substrato formulado com solo de cerrado e esterco bovino proporcionou o melhor desenvolvimento das mudas de calêndula. Contudo, os diferentes tipos de substrato e bandejas de poliestireno não influenciaram significativamente a produção de capítulos das mudas transplantadas.Marigold Calendula (Calendula officinalis L. is an annual medicinal plant of Mediterranean origin which presents anti-inflammatory, antiviral and antigenotoxic properties. This species normally propagates through seeds, and the use of polystyrene trays is an economic way to produce its seedlings. Two experiments were carried out to evaluate the

  5. Controlled release of an extract of Calendula officinalis flowers from a system based on the incorporation of gelatin-collagen microparticles into collagen I scaffolds: design and in vitro performance.

    Science.gov (United States)

    Jiménez, Ronald A; Millán, Diana; Suesca, Edward; Sosnik, Alejandro; Fontanilla, Marta R

    2015-06-01

    Aiming to develop biological skin dresses with improved performance in the treatment of skin wounds, acellular collagen I scaffolds were modified with polymeric microparticles and the subsequent loading of a hydroglycolic extract of Calendula officinalis flowers. Microparticles made of gelatin-collagen were produced by a water-in-oil emulsion/cross-linking method. Thereafter, these microparticles were mixed with collagen suspensions at three increasing concentrations and the resulting mixtures lyophilized to make microparticle-loaded porous collagen scaffolds. Resistance to enzymatic degradation, ability to associate with the C. officinalis extract, and the extract release profile of the three gelatin-collagen microparticle-scaffold prototypes were assessed in vitro and compared to collagen scaffolds without microparticles used as control. Data indicated that the incorporation of gelatin-collagen microparticles increased the resistance of the scaffolds to in vitro enzymatic degradation, as well as their association with the C. officinalis flower extract. In addition, a sharp decrease in cytotoxicity, as well as more prolonged release of the extract, was attained. Overall results support the potential of these systems to develop innovative dermal substitutes with improved features. Furthermore, the gelatin-collagen mixture represents a low-cost and scalable alternative with high clinical transferability, especially appealing in developing countries.

  6. Preclinical evaluation of collagen type I scaffolds, including gelatin-collagen microparticles and loaded with a hydroglycolic Calendula officinalis extract in a lagomorph model of full-thickness skin wound.

    Science.gov (United States)

    Millán, D; Jiménez, R A; Nieto, L E; Linero, I; Laverde, M; Fontanilla, M R

    2016-02-01

    Previously, we have developed collagen type I scaffolds including microparticles of gelatin-collagen type I (SGC) that are able to control the release of a hydroglycolic extract of the Calendula officinalis flower. The main goal of the present work was to carry out the preclinical evaluation of SGC alone or loaded with the C. officinalis extract (SGC-E) in a lagomorph model of full-thickness skin wound. A total of 39 rabbits were distributed in three groups, of 13 animals each. The first group was used to compare wound healing by secondary intention (control) with wound healing observed when wounds were grafted with SGC alone. Comparison of control wounds with wounds grafted with SGC-E was performed in the second group, and comparison of wounds grafted with SGC with wounds grafted with SGC-E was performed in the third group. Clinical follow-ups were carried in all animals after surgery, and histological and histomorphometric analyses were performed on tissues taken from the healed area and healthy surrounding tissue. Histological and histomorphometric results indicate that grafting of SGC alone favors wound healing and brings a better clinical outcome than grafting SGC-E. In vitro collagenase digestion data suggested that the association of the C. officinalis extract to SGC increased the SGC-E cross-linking, making it difficult to degrade and affecting its biocompatibility.

  7. Effects of different nitrogen levels and plant density on flower, essential oils and extract production and nitrogen use efficiency of Marigold (Calendula officinalis.

    Directory of Open Access Journals (Sweden)

    ali akbar ameri

    2009-06-01

    Full Text Available Efficient use of nitrogen for medicinal plants production, might increase flower dry matter, essential oil and extract yield and reduce cost of yield production. A two year (2005 and 2006 field study was conducted in Torogh region(36,10° N,59.33° E and 1300 m altitude of Mashhad, Iran, to observe the effects of different nitrogen and densities on flower dry matter, essential oil and extract production and nitrogen use efficiency (NUE in a multi-harvested Marigold (Calendula officinalis. The levels of Nitrogen fertilizer (N were 0, 50, 100 and 150 kg ha-1 and levels of density were 20, 40, 60 and 80 plant m-2. The combined analysis results revealed significant effects of N and density levels on flower dry matter, essential oil and extract production and NUE of Marigold. The highest dry flower production obtained by 150 kg ha-1 N and 80 plant m-2 plant population (102.86 g m-2. The higher flower dry matter production caused more essential oil and extract production in high nitrogen and density levels. Agronomic N-use efficiency (kg flower dry matter yield per kg N applied, physiological efficiency (kg flower dry matter yield per kg N absorbed and fertilizer N-recovery efficiency (kg N absorbed per kg N applied, expressed as % for marigold across treatments ranged from 6.8 to14.9, 12.3 to 33.6 and 55.5 to 77.6, respectively and all were greater for N application at 50 compared with150 kg N ha-1, and under high density than low density. The amount of essential oil and extract per 100g flower dry matter decreased during the flower harvesting period. The higher amount of essential oil and extract obtained at early flowering season. The essential oil and extract ranged from 0.22 to 0.12 (ml. per 100g flower dry matter and 2.74 to 2.13 (g per 100g flower dry matter respectively.

  8. Influence of Foliage Fertilizer Application on the Growth and Development of Calendula officinalis%叶面喷肥对金盏菊生长发育的影响

    Institute of Scientific and Technical Information of China (English)

    谭家伟

    2011-01-01

    Experiments of foliage fertilizer application were done with different concentrations of KH2PO4. The results showed that 2‰ KH2PO4 can not only promote the growth of the seedlings, but also advance the flowering period, increase the yield and quality of the flowers and chlorophyll content, satisfying the long-term nutritional needs of Calendula officinalis for its growth. The phosphorus and potassium fertilizer is suitable for the growth and development of potted plants of Calendula offwinalis.%选用不同浓度的KH2PO4分别对盆栽金盏菊进行了叶面喷肥试验。结果表明:2‰KH2PO4不仅可促进金盏菊幼苗生长,还可提旱花期,提高花的产量及品质,增加叶绿素含量,能满足金盏菊生长对养分的长期需求,即磷钾肥更适于盆栽金盏菊的生长发育。

  9. Influência da adubação e da cobertura morta na produtividade e no teor de flavonóides de Calendula officinalis L. (Asteraceae Influence of fertilization and mulching on the productivity and flavonoid content of Calendula officinalis L. (Asteraceae

    Directory of Open Access Journals (Sweden)

    J.C Borella

    2011-01-01

    Full Text Available Desenvolveu-se ensaio de cultivo de Calendula officinalis, variando-se os tipos de adubação e utilização de cobertura morta na observação da produção de inflorescências, droga vegetal e quantificação dos flavonóides presentes nestes materiais.O delineamento experimental adotado foi de blocos casualizados com 3 repetições, em arranjo fatorial de 3 x 2, constituído de seis tratamentos, sendo 1. adubação química (100 kg de N ha-1 + 50 kg de P ha-1; 2. adubação química + cobertura morta (grama seca - Paspalum notatum; 3. adubação orgânica (esterco bovino curtido 50 t ha-1; 4. adubação orgânica + cobertura morta; 5. sem adubação química ou orgânica + cobertura morta; 6. sem adubação química ou orgânica e sem cobertura morta (testemunha. Os resultados de número capítulos produzidos, massa seca das inflorescências e massa da droga vegetal foram totalizados após cinco meses do plantio. A quantificação de flavonóides foi realizada através de método espectrofotométrico. Os dados foram analisados estatisticamente (ANOVA/Teste Tukey-Kramer-5% e mostraram diferenças significativas (70% superior no número de inflorescências, massa seca das inflorescências e massa da droga vegetal entre o tratamento com adubação orgânica (3 e os tratamentos sem adubação química ou orgânica com cobertura morta (5 e o testemunha (6. As demais comparações, inclusive o teor de flavonóides, entre os diversos tratamentos não resultaram em diferenças significativas.Calendula officinalis was experimentally grown under field conditions with different fertilization types and mulching in order to evaluate inflorescence production, plant drugs and flavonoid levels. Experimental design was in randomized blocks, with 3 replicates, in a 3x2 factorial arrangement, consisting of six treatments, as follows: 1. chemical fertilization (100 kg N ha-1 + 50 kg P ha-1; 2. chemical fertilization + mulch (dry grass - Paspalum notatum; 3

  10. Variation in seed lipids in Calendula germplasm

    Science.gov (United States)

    Calendula officinalis (pot marigold) has considerable promise as an industrial crop, with a long history as an ornamental and medicinal plant. It is also marketed as an ingredient in cosmetics and a colorant. It produces unusual seed lipids, which can provide an additional market for commercial Ca...

  11. Physical chemistry evaluation of stability, spreadability, in vitro antioxidant, and photo-protective capacities of topical formulations containing Calendula officinalis L. leaf extract

    Directory of Open Access Journals (Sweden)

    Viviane Cecília Kessler Nunes Deuschle

    2015-03-01

    Full Text Available Calendula is used widely in cosmetic formulations that present phenolic compounds in their chemical constitution. The objective of our research was to develop and evaluate the stability of topical formulations containing 5% hydro-ethanolic extract of calendula leaves, including spreadability, and in vitro photo-protective, and antioxidant capacity. To evaluate the stability, we used organoleptic characteristics, pH, and viscosity parameters. Antioxidant capacity was measured by the DPPH (2,2-diphenyl-1-picrylhydrazyl method, and the photo-protective capacity by SPF spectrophotometric measure. All formulations were stable. The calendula extract formulations in gel and cream showed no significant variations in pH, and the cream formulations presented lower viscosity variations than gel formulations. The spreadability of the gel formulations was superior to those in cream. The formulations also presented good antioxidant capacities and an FPS of around 1.75. In accordance with the results, the formulations can be used as antioxidants, but considering the low SPF obtained, calendula cannot be considered as a stand-alone sunscreen, yet may well be tested in future studies towards verifying enhancement of synthetic sunscreens.

  12. Effect of intercropping carrot (Daucus carota L. with french marigold (Tagetes patula nana L. and pot marigold (Calendula officinalis L. on the occurrence of some pests and quality of carrot yield

    Directory of Open Access Journals (Sweden)

    Beata Jankowska

    2012-12-01

    Full Text Available Intercropping combines different aspects of the interaction between organisms in ecosystems and may be classified as a pro-ecological method of plant cultivation limiting the harmful human interference in the environment, especially the use of chemicals. It also allows high yield, good quality, and economic productivity to be achieved. The aim of the present study, conducted in the years 2003–2004 in Mydlniki near Kraków, was to determine the effect of intercropping carrot Nardin F1 with French marigold (Tagetes patula nana L. 'Kolombina' and pot marigold (Calendula officinalis L. 'Promyk' on carrot yield, its quality, and the occurrence of pests. On average in both years of the study, intercropping did not reduce total or marketable carrot yield. In 2003 intercropping contributed to a significant increase in marketable yield compared with yield from sole cropping. In 2004 the dry matter content was higher in the roots of carrots grown with pot marigold. The treatments intercropped with both French and pot marigold were characterized by an increased content of sugars in carrot roots and in 2004 year – of carotenoids. Intercropping had a significant effect on the number of roots damaged by the carrot rust fly, Psila rosae, (the best in this respect was the combination with Tagetes and by nematodes (the best in this respect was the combination with Calendula. The number of larvae of carrot psyllid, Trioza viridula, was significantly lower in the plots where carrot was intercropped. The study did not find intercropping to have a positive effect on reducing the occurrence of the root aphid.

  13. ESSENTIAL OIL OF CALENDULA OFFICINALIS

    Directory of Open Access Journals (Sweden)

    Aziza Juraeva

    2014-11-01

    Full Text Available The article presents the results of research into component composition of raw calendula’s essential oil. The study was conducted by gas chromatography mass spectrophotometry method under the following conditions: column - CP-Wax58FFAPCB24,5 mmx 250mm x0,20 mmnominal, the mobile-phase-He (helium, speed-column mobile phase-1.0 ml /min, volume sample injection-5mkl; resolutions(5:1, in the Split, high-boiling chamber -220 ◦C, detector temperature 275◦C; Aux-200 ◦ C, column temperature 70C◦ gradually increased from 10◦C / min to 250◦C, maintained to the end of analysis temperature regime(250◦Cfor 5min.In the course of the research the following substances were indentified - 1H cyclopropazulen-0, 981%, α-amorphous-1, 926%, γ-cadinene-11, 598%, 1H-cyclopropazulen 4-oldecahydro-1-0.825% viridiflorol-2, 029%, β-cubeb -3.809%, T-1 muurololnaftalenol-8.522%.In addition, it was ascertained that in different parts of the world calendula’s essential oils differ from each other in qualitative composition and quantitative components.

  14. Avaliação do tratamento subcrônico com o extrato hidroalcoólico de Calendula officinalis L. sobre os parâmetros bioquímicos e hematológicos em ratas Wistar

    Directory of Open Access Journals (Sweden)

    E.J.R. Silva

    2005-06-01

    Full Text Available Os efeitos da administração oral subcrônica do extrato hidroalcoólico (EHA preparado de flores de Calendula officinalis L. foram investigados sobre os parâmetros hematológicos e bioquímicos em ratas Wistar adultas. Quarenta ratas (n=10/grupo foram tratadas durante 30 dias consecutivos com EHA por via oral nas doses de 0,25, 0,5, e 1,0 g/kg de peso e, em seguida, determinados os perfis bioquímico e hematológico e a massa dos órgãos. Os resultados mostram que durante o período do tratamento não se observou sinais de toxicidade ou morte. Os parâmetros bioquímicos e hematológicos, assim como a massa dos órgãos não foram modificados pela administração subcrônica do EHA, excetuando-se aumento significativo de 24,2% para uréia na maior dose estudada e aumento, respectivamente, de 62,3, 30,2 e 44,4%, para ALT. Na hematologia, registrou-se flutuação dentro dos valores de referência na contagem diferencial de neutrófilos, linfócitos e monócitos. Dessa forma, a administração subcrônica do extrato hidroalcoólico de Calendula officinalis não produz efeitos tóxicos sobre a maioria dos parâmetros bioquímicos e hematológicos estudados em ratas Wistar adultas. Entretanto, o aumento dos níveis séricos de uréia e alanina aminotransferase (ALT em doses elevadas sugere sobrecargas renal e hepática, respectivamente, as quais devem ser investigadas em maiores detalhes.

  15. Mastering analytical challenges for the characterization of pentacyclic triterpene mono- and diesters of Calendula officinalis flowers by non-aqueous C30 HPLC and hyphenation with APCI-QTOF-MS.

    Science.gov (United States)

    Nicolaus, Christoph; Sievers-Engler, Adrian; Murillo, Renato; D'Ambrosio, Michele; Lämmerhofer, Michael; Merfort, Irmgard

    2016-01-25

    Pentacyclic triterpene mono- and diesters have been isolated from Calendula officinalis flowers. GC-MS, APCI-Exactive Orbitrap HR-MS and NMR allowed to identify the triterpene skeleton in various samples (different triterpene mixtures from Calendula n-hexane extract). NMR provided evidence that triterpene diesters are present in the samples as well. However, the corresponding quasi-molecular ions could not be detected by APCI-Exactive Orbitrap HR-MS. Instability of triterpene diesters and loss of a fatty acid residue, respectively, in the ion-source made their MS detection challenging. Thus, a set of new APCI-QTOF-MS methods (using the TripleTOF 5600+ mass spectrometer) were developed which made it eventually possible to solve this problem and confirm the diester structures by MS via quasi-molecular ion [M+H](+) detection. Direct infusion APCI-QTOF MS experiments in MS/MS high sensitivity scan mode with low collision energy and multi-channel averaging acquisition (MCA) allowed the detection of quasi-molecular ions of triterpene diesters for the first time and unequivocally confirmed the presence of faradiol 3,16-dimyristate and -dipalmitate, as well as the corresponding mixed diesters faradiol 3-myristate,16-palmitate and faradiol 3-palmitate,16-myristate. Preferential loss of the fatty acid in 16-position made it possible to distinguish the mixed diesters by MS/MS spectra. Their chromatographic separations turned out to be challenging due to their bulkiness and extended molecular dimensions. However, separation could be achieved by an uncommon non-aqueous RPLC mode with an in-house synthesized C30 phase. Finally, two (U)HPLC-APCI-QTOF-MS methods with C18- and C30-based non-aqueous RPLC provided suitable, sensitive assays to monitor the presence of monoesters and diesters of various triterpenes (faradiol, maniladiol, arnidiol, arnitriol A and lupane-3β,16β,20-triol esters) in the n-hexane extract of C. officinalis with high mass resolution and good mass accuracy.

  16. EFFECTS OF HORMONES ADDITION FOR IN VITRO PLANT DEVELOPMENT OF CALENDULLA OFFICINALIS

    Directory of Open Access Journals (Sweden)

    IRINA-CLAUDIA ALEXA

    2015-05-01

    Full Text Available The current study presented in vitro plant development of Calendula officinalis on MS (Murashige and Skoog medium supplemented with different formula of hormones. The morphogenetic response was evaluated by recording the number of plantlets, the plant height and weight. The results showed that small amounts of hormones can improve the plantlets growth in the case of Calendula officinalis.

  17. Effect of Exogenous Matters on Development of Calendula officinalis under Seawater Stress%外源物影响海水胁迫金盏菊发育的效应

    Institute of Scientific and Technical Information of China (English)

    吴文杰; 王琼

    2010-01-01

    以金盏菊(Calendula officinalis)为材料,研究甜菜碱(Glycinebetaine,GB)和硫酸锌(ZnSO4)对遭受35%海水胁迫的金盏菊幼苗的形态建成及其生理生化特性的影响.结果表明:外源GB(100~300 mg/L)和ZnSO4(1×10-5mol/L)可以促进金盏菊种子的萌发和幼苗的形态建成,促进叶中叶绿素含量的积累,降低根系内丙二醛(MDA)含量,提高过氧化物酶(POD)的活性,增强植物的渗透调节能力,缓解海水盐分胁迫对金盏菊幼苗的伤害.

  18. 陇东黄土高原石油污染土壤环境因子对金盏菊(Calendula officinalis)-微生物联合修复的响应%Environmental factors responses to petroleum-contaminated soil in situ remediation by using a combination of Calendula officinalis and microbial agent in eastern Gansu Province

    Institute of Scientific and Technical Information of China (English)

    王金成; 井明博; 段春燕; 张希彪; 刘建新; 肖朝霞; 周天林

    2015-01-01

    为筛选适合陇东黄土高原石油污染土壤修复植物,选取当地“适生”植物金盏菊(Calendula officinalis)联合微生物菌剂开展了为期5个月的原位修复实验.采用常规方法和PCR-DGGE技术分析了修复过程中土壤理化性质、酶活性及土壤微生物群落遗传多样性等15项土壤环境因子的动态变化情况及其对金盏菊生态修复的响应.结果表明,随着原位修复的进行,①土壤脲酶、多酚氧化酶、过氧化氢酶及脱氢酶活性均不同程度呈增加的趋势(p<0.05).②土壤TPHs、pH、有机质、含盐率的变化情况不尽相同,但总体呈下降的趋势(p<0.05);土壤碱解氮、速效磷及速效钾含量总体呈上升趋势(p<0.05).③土壤微生物群落遗传多样性结果显示,Shannon-Wiener多样性指数和Pielou均匀度指数整体呈逐步增加的趋势.④PCA分析结果显示,土壤脲酶、脱氢酶及碱解氮等环境因子对金盏菊修复表现为促进作用,而有机质含量过高将不利于金盏菊的植物修复.金盏菊和微生物菌剂联合修复过程中,土壤多酚氧化酶、速效磷、脱氢酶及速效钾发挥主要作用,而土壤TPHs和含盐率过高则影响了联合修复效果.本研究旨在分析金盏菊用于陇东地区油污土壤的生态响应,结果有助于筛选出适合陇东黄土高原土壤类别-适生植物-土著降解菌群的组合,利用乡土物种的适生性降低修复成本,为陇东地区油污土壤生态修复提供新的技术方案和背景资料.

  19. A Review of the Use of Topical Calendula in the Prevention and Treatment of Radiotherapy-Induced Skin Reactions.

    Science.gov (United States)

    Kodiyan, Joyson; Amber, Kyle T

    2015-04-23

    Calendula is a topical agent derived from a plant of the marigold family Calendula Officinalis. Containing numerous polyphenolic antioxidants, calendula has been studied in both the laboratory and clinical setting for the use in treating and preventing radiation induced skin toxicity. Despite strong evidence in the laboratory supporting calendula's mechanism of action in preventing radiation induced skin toxicity, clinical studies have demonstrated mixed results. In light of the controversy surrounding the efficacy of calendula in treating and preventing radiodermatitis, the topic warrants further discussion.

  20. The Effects of Leaf-Spraying Urea and Calcium Superphosphate on the Growth and Deveplpment of Calendula officinalis L.%叶面喷施尿素和过磷酸钙对金盏菊生长发育的影响

    Institute of Scientific and Technical Information of China (English)

    曾长立; 程辉; 张萍

    2004-01-01

    研究了在金盏菊(Calendula officinalis L.)的生长初期叶面喷施各种不同浓度配比的尿素和过磷酸钙对其营养性状和花发育的影响,结果表明:尿素和过磷酸钙配合进行叶面喷施,能促进生长,增加株高、叶片数及花朵数,其中以0.1%的尿素和1.0%的过磷酸钙配合效果最佳.

  1. A review on phytochemistry and ethnopharmacological aspects of genus Calendula.

    Science.gov (United States)

    Arora, Disha; Rani, Anita; Sharma, Anupam

    2013-07-01

    This review includes 84 references on the genus Calendula (Asteraceae) and comprises ethnopharmacology, morphology and microscopy, phytoconstituents, pharmacological reports, clinical studies and toxicology of the prominent species of Calendula. Triterpene alcohols, triterpene saponins, flavonoids, carotenoids and polysaccharides constitute major classes of phytoconstituents of the genus. A few species of this genus have medicinal value, among these Calendula officinalis Linn., has been traditionally used in the treatment of various skin tumors, dermatological lesions, ulcers, swellings and nervous disorders as well as almost 200 cosmetic formulations, i.e., creams, lotions, shampoos. Despite a long tradition of use of some species, the genus has not been explored properly. In the concluding part, the future scope of Calendula species has been emphasized with a view to establish their multifarious biological activities and mode of action.

  2. 湿法消解火焰原子吸收法测定黄土复合污染修复植物金盏菊幼苗中的铅和镉%Determination of Lead and Cadmium in Calendula Officinalis Seedlings for Phytoremediation of Multi-Contaminated Loess by Using Flame Atomic Absorption Spectrometry with Wet Digestion

    Institute of Scientific and Technical Information of China (English)

    范春辉; 杜波; 张颖超; 高雅琳; 常敏

    2016-01-01

    Atomic absorption spectrometry (AAS)is widely used in the analysis and detection of heavy metals.The optimal operation conditions on metals detection are important for the stabilization and repeatability of scientific research,which affects the expected inves-tigation objectives greatly.Thus,it becomes a key issue to establish appropriate detection methods in scientific works.Calendula offi-cinalis was used for lead/cadmium remediation in contaminated loess,and the flame atomic absorption spectrometry (FAAS)with wet digestion was applied to analyze the contents of lead/cadmium in Calendula officinalis seedlings.The investigation data was further studied to reveal the bioaccumulation efficiency of lead/cadmium in Calendula officinalis seedlings.The results showed:the limits of detection are 0.104 and 0.007 mg·L-1 for lead and cadmium;the recovery rates of lead and cadmium are from 94.33% to 110.78%and 97.73% to 107.50% respectively.The relative standard deviations (RSD)are between 4.11% and 4.75% for lead while between 1.11% and 2.77% for cadmium,and It’s been proved that the method is accurate and reliable.The low accumulation efficiency of lead with Calendula officinalis seedlings might be related to the electronegativity of lead while the growth period of calendula officinalis seedlings and environmental factors.The accumulation content of cadmium is 104.85 mg·kg-1 with cadmium concentration of 50 mg· kg-1 .The co-existing of lead in loess is positive for cadmium accumulation by Calendula officinalis seedlings,and the synergistic effect might work in the process.The established detection method is effective for quantitative analysis of lead and cadmium in calendula offi-cinalis seedlings and significant for future research.%原子吸收光谱法(AAS)广泛应用于重金属分析检测领域,优化测试过程的操作条件,进而保障分析结果的稳定性和重现性,直接关系到预期研究目标的有效实现,建立精准的

  3. A Review of the Use of Topical Calendula in the Prevention and Treatment of Radiotherapy-Induced Skin Reactions

    Directory of Open Access Journals (Sweden)

    Joyson Kodiyan

    2015-04-01

    Full Text Available Calendula is a topical agent derived from a plant of the marigold family Calendula Officinalis. Containing numerous polyphenolic antioxidants, calendula has been studied in both the laboratory and clinical setting for the use in treating and preventing radiation induced skin toxicity. Despite strong evidence in the laboratory supporting calendula’s mechanism of action in preventing radiation induced skin toxicity, clinical studies have demonstrated mixed results. In light of the controversy surrounding the efficacy of calendula in treating and preventing radiodermatitis, the topic warrants further discussion.

  4. Deficiência hídrica e aplicação de ABA nas trocas gasosas e no acúmulo de flavonoides em calêndula (Calendula officinalis L. = Water deficit and ABA application on leaf gas exchange and flavonoid content in marigold (Calendula officinalis L..

    Directory of Open Access Journals (Sweden)

    Ana Cláudia Pacheco

    2011-04-01

    Full Text Available O objetivo deste trabalho foi avaliar os efeitos da deficiencia hidrica e aplicacao de acido abscisico (ABA sobre aspectos fisiologicos e teor de flavonoides em plantas de calendula. Oexperimento foi instalado em condicoes de casa-de-vegetacao com plantas envasadas. No inicio do florescimento de plantas de calendula, foram aplicados quatro intervalos de suspensao da irrigacao (irrigacao diaria; tres; seis e nove dias sem irrigar, acompanhados por tres doses de ABA (0, 10 e 100 ƒÊM. Avaliou-se o conteudo relativo de agua na folha (CRA e as trocas gasosas, utilizando-se um analisador portatil por infravermelho (A: fotossintese liquida, gs: condutancia estomatica, E: transpiracao, Ci: concentracao intercelular de CO2 e EUA: eficiencia de uso daagua. Aos nove dias sem irrigacao ocorreram reducoes significativas em todas as variaveis de trocas gasosas analisadas, independente da aplicacao de ABA. Concluiu-se que o efeito principal do ABA foi o de causar diminuicao na gs, a qual foi acompanhada de reducao em A somente quando as plantas estavam desidratadas. As intensidades de deficiencia hidrica testadas nao causaram interferencia no acumulo de flavonoides nas inflorescencias. Entretanto, o ABA restringiu a biossintese de flavonoides, tanto nas plantas-controle como nas plantas submetidas a deficiencia hidrica.The goal of this study was to evaluate the effects of water deficit and abscisic acid (ABA application on physiological parameters and flavonoid production in marigold plant. The experiment was performed under nursery conditions with potted plants. It was tested water deficit by withholding water (control . diary irrigation, 3, 6 and9 days without irrigation followed by 3 ABA concentrations (0, 10 e 100 ƒÊM applied in the beginning of blooming. It was evaluated the relative water content and the leaf gas exchange using a portable infrared gas analyzer (A: net photosynthesis, gs: stomatal conductance, E: transpiration, Ci: CO2 intercellular

  5. Postemergence herbicides for calendula

    Science.gov (United States)

    Calendula is an alternative oilseed crop whose seed oil is valued as a substitute for tung oil and a replacement for petroleum-based volatile organic compounds in paints and other coatings. Calendula is not yet grown extensively as an agronomic crop, and its tolerances to most herbicides are unknown...

  6. The growth and bioaccumulation characteristic of Calendula officinalis L under lead/cadmium co-contamination%铅/镉复合污染物对金盏菊生长的影响及在其体内富集特征

    Institute of Scientific and Technical Information of China (English)

    丁绍兰; 杜波; 范春辉

    2015-01-01

    花卉类植物的抗逆环境能力较强,生长周期较短、生物量较大;利用花卉类植物修复重金属污染土壤,有效避免了食物链循环效应对人体健康的潜在威胁,因此,该修复技术的安全性较好、实用性较强。通过盆栽试验研究Pb/Cd复合污染对金盏菊(Calendula officinalis L )幼苗生长状况的影响,同时考察土壤持水量对Pb/Cd富集行为的影响。研究发现:污染胁迫条件下,金盏菊幼苗的出苗率都维持在45%以上;黄土Pb/Cd对金盏菊生物量的影响不大,金盏菊幼苗的生长过程能够一定程度上改良黄土理化性质。通过扫描电镜发现,幼苗根部较空白对照组发生显著改变,以适应重金属的复合胁迫作用。此外,过高或过低的土壤持水量都不利于金盏菊幼苗对Pb/Cd的吸收积累,根部是金盏菊幼苗积累Cd的主要部位,且金盏菊幼苗对黄土中Cd的萃取能力较强。%Having a short grow th periodicity and a strong enrichment in biomass ,the adaption to adverse environment of ornamental plants is quite strong ,and the circulatory effect of food chain ,a potential threat to human health ,could be effectively avoided w hen using ornamental plants to restore contaminated soil with heavy metal ,so this technology are proved security and practicability .The pot experiments were conducted to study the effect of Pb/Cd com‐bined pollution on the grow th of Calendula officinalis L and the effect of soil moisture on the bioaccumulation behavior of the seedlings .The results indicated that the emergence rate remained 45% or more under the contamination condition ,the lead/cadmium in loess had a little effect on the biomass of Calendula officinalis L ,and the grow th process of seedlings could improve partly physical and chemical properties of contaminated loess .Compared with the CK ,the root morphology of seedlings was significantly changed by SEM so that Calendu

  7. FTIR Analysis of Secondary Abiotic Stress Response of Calendula Officinalis Seedlings to Lead and Cadmium in Multi-Contaminated Loess%黄土区金盏菊幼苗对 Pb/Cd 次生非生物胁迫响应的红外光谱研究

    Institute of Scientific and Technical Information of China (English)

    范春辉; 常敏; 张颖超; 高雅琳

    2016-01-01

    -vanced instruments are effective to resolve the scientific issues,while the related researches are seldom investigated.Taking ca-lendula officinalis seedlings as experimental samples,scanning electron microscope (SEM)and Fourier transform infrared spectroscopy (FT-IR)were applied to compare the surface characteristics and functional groups variation,respectively.The re-sponse process and tolerance mechanism of calendula officinalis seedlings to lead and cadmium stress were further analyzed. The results showed:with the increasing concentration of lead and cadmium in loess,the calyptra of calendula officinalis seed-lings become bending and wilting;the amount of root hairs decreases greatly while no significant variations of aerial parts are found on surface characteristics.The stress of lead and cadmium are responsible for the difference of FTIR spectra of Calendula officinalis seedlings.The peak absorbance at 3 573 cm-1 decreases and the peak becomes more complex with the increasing con-tents of lead and cadmium.It might be the coordination effect between lead/cadmium and hydroxyl,which affects the synthesis and secretion of organic matters.The movement of C—H vibration peak is associated with the lipid oxidationwhile the changes at 1 631 and 1 574 cm-1 suggest the protein component differences.The shifting peak at 1 385 cm-1 might be associated with the methylation of pectin and lipid,and the behavior is positive for the adaption process of calendula officinalis seedlings to lead and cadmium stress.The FTIR spectra are effective to reveal the phytoremediation mechanism on heavy metals contamination in pedosphere.

  8. Metabolism of [3-{sup 3}H]oleanolic acid in the isolated ``Calendula officinalis`` leaf cells and transport of the synthesized glycosides, to the cell wall and the extracellular space

    Energy Technology Data Exchange (ETDEWEB)

    Szakiel, A.; Wasiukiewicz, I.; Janiszowska, W. [Warsaw Univ. (Poland). Katedra Biochemii

    1995-12-31

    It has been shown for the first time that [3-{sup 3}H]oleanolic acid glycosides formed in the cytosol of ``C. officinalis`` leaf cells are transported to the extracellular space in the form of pentaglucoside VI (44%), whereas glucuronides derived from [3-{sup 3}H]oleanolic acid 3-O-monoglucuronide (29%) as well as a part of glucosides (24%) were transported into the cell walls. (author). 15 refs, 2 figs, 1 tab.

  9. 施肥对金盏菊生长、开花及养分吸收的影响%Effects of Nitrogen,Phosphorus,and Potassium Fertilizer on the Growth,Blossom,and N,P,K Uptake in Calendula officinalis L.

    Institute of Scientific and Technical Information of China (English)

    张文君; 鲁剑巍; 蒋志平; 王亚艺

    2010-01-01

    研究了氮、磷、钾不同施肥处理对金盏菊(Calendula officinalis L.)生长、开花、干物质积累量及养分吸收的影响.结果表明:推荐施肥[N-P2O5-K2O:(0.4-0.2-0.3) g/kg]能有效促进金盏菊的生长及花朵开放,其中干物质积累量和总花朵数,分别比不施肥处理增加了264. 3%和323. 1%,比习惯施肥增加了16.0%和25.0%;不施肥、习惯施肥和推荐施肥三者干物质日积累量高峰均出现在移栽后29~35 d,分别为0. 15、 0. 43、 0.42 g/d;各处理氮、磷、钾吸收量之间均存在显著差异,推荐施肥处理N、P、K养分累积量分别为0.33、 0.04、 0.39 g/株,分别是不施肥处理的6.93倍、 3.94倍和3.61倍,为习惯施肥的1.49倍、 1.35倍和1.24倍,各处理金盏菊对N、P、K养分吸收比例变幅为1∶(0.13~0.24)∶(1.20~2.30),平均比例为1∶0. 17∶1. 65.

  10. 转 merA 基因金盏菊对环境中汞污染的修复效果%Remediation Effect of Transgenic Calendula officinalis with merA Gene on Mercury Pollution

    Institute of Scientific and Technical Information of China (English)

    张雷; 丁艳丽; 付涌玉

    2015-01-01

    The remediation effect of transgenic C.officinalis with merA gene obtained by Agrobacterium-mediated genetic transformation system on mercury pollution was analyzed to study the mercury enrichment rate.The results showed that the 84% Hg2+ in soil can be transported into leaves of transgenic C.officinalis with merA gene and then most Hg2+ in leaves can be converted into nontoxic Hg under the action of merA gene,which can increase its tolerance to HgCl2 stress.The proline and MDA content,and activity of SOD and POD of transgenic C.officinalis with merA gene are increased under Hg2+ stress to varying degrees.In conclusion,transgenic C.officinalis with merA gene has the higher tolerance to Hg2+ stress.%为了提高金盏菊植物对环境中重金属(Hg)污染的修复效果,构建 merA 重组表达载体质粒,再经农杆菌介导法建立遗传转化体系筛选出转 merA 基因的金盏菊植株,研究其对汞的富集率。结果表明:转 merA 基因金盏菊对土壤中 Hg2+的转移率提高84%,而且大部分 Hg2+在 merA 基因作用下,转化为无毒的 Hg 挥发到空气中,提高了其忍受 HgCl2的浓度;在 Hg2+胁迫下,转基因植株中脯氨酸含量、SOD 酶活性、POD 酶活性及 MDA 含量均有不同程度的提高。转基因金盏菊具有较强的抗重金属汞胁迫的能力。

  11. Effect of fertilisation and harvest period on polar metabolites of Calendula oficcinalis

    Directory of Open Access Journals (Sweden)

    Eduardo Felipe Alves Fernandes

    2013-10-01

    Full Text Available The present study evaluated the chemical profile of polar extracts of Calendula officinalis L., Asteraceae, that were grown under different cultivation conditions: chemical fertilisation, organic fertilisation and mulching. Furthermore, we investigated metabolite variations during plant development by comparing the metabolites from harvested plants at 60 and 120 days after planting. We used HPLC-DAD-MS/MS to tentatively identify metabolites. In total, we identified seven known compounds: five flavonoid glycosides and two caffeoylquinic acids derivatives. There were no statistically significant differences in the expression of metabolites from plants grown under the examined soil treatments. However, five substances varied according to harvest time, suggesting that the biosynthesis of polar metabolites of Calendula officinalis is not affected by changes in soil composition. Therefore, this plant could represent a source for phytomedicines with a constant content of polar metabolites.

  12. Growth of Pelargonium zonale and Calendula officinalis when utilizing green waste compost as a peat substitute%园林废弃物堆肥替代泥炭用于天竺葵和金盏菊栽培

    Institute of Scientific and Technical Information of China (English)

    魏乐; 李素艳; 李燕; 龚小强; 孙向阳

    2016-01-01

    为探索园林废弃物堆肥替代泥炭用于天竺葵Pelargonium zonale和金盏菊Calendula officinalis栽培基质的可能性,将改良后的园林废弃物堆肥分别以0%,25%,50%,75%和100%的比例替代泥炭进行试验.将天竺葵和金盏菊的种子分别播种在不同基质中进行育苗研究,当幼苗生长35 d后进行测定.将幼苗分别移栽到不同基质中,栽培6个月后进行测定.结果表明:随园林废弃物比例的增加,基质的容重增加,总孔隙度度、通气孔隙度和持水孔隙度呈下降趋势.园林废弃物比例的增加提高了基质pH值、电导率值、大量元素和微量元素质量分数.育苗试验显示:天竺葵幼苗在园林废弃物处理中的生长量显著(P<0.05)低于对照组,即使在25%的低比例下也会对幼苗产生不良影响.金盏菊幼苗在添加25%和50%的园林废弃物处理中的生长量与对照组一致.盆栽试验显示:添加园林废弃物显著(P<0.05)增加了天竺葵和金盏菊的地上部分干质量、株高、茎直径和叶面积以及金盏菊的根干质量和花蕾数.园林废弃物堆肥可以部分代替泥炭作为金盏菊育苗基质及天竺葵和金盏菊的栽培基质.

  13. [Antimicrobial activity of Calendula L. plants].

    Science.gov (United States)

    Radioza, S A; Iurchak, L D

    2007-01-01

    The sap of different organs of genus Calendula plant species has been studied for antimicrobial activity. The sap of racemes demonstrated the most expressed antimicrobial effect while that of the roots - the least one. Calendula species inhibited all tested pathogenic microorganisms, especially Pseudomonas syringae, P. fluorescens, Xanthomonas campestris, Agrobacterium tumefaciens. Calendula suffruticosa was the most active to all investigated microorganisms.

  14. Spectral Evidence of In-Situ Regulatory Mechanisms on Chemical Speciation of Lead/Cadmium Affected by Dissolved Organic Matter Extracted from Rhizosphere Loess of Calendula Officinalis Seedling%金盏菊根际圈黄土溶解性有机质对Pb/Cd赋存形态原位调控机制的光谱学证据

    Institute of Scientific and Technical Information of China (English)

    范春辉; 徐慧荟; 张颖超

    2016-01-01

    The various characteristics of DOM have great impact on the transformation process of contaminants in soil due to the generation of the feedback regulation in cycles of plant,Rhizosphere soil and environmental contaminants.Currently,more at-tention was paid on contaminants behaviors of adsorption,transformation,accumulation and detoxification than DOM derived from Rhizosphere soil.The chemical speciation of lead/cadmium and growth efficiency related variations were revealed in the growth process of Calendulaofficinalis seedlings,and the spectral characteristics of DOM in Rhizosphere loess were discussed with UV,FT-IR and 3D-EEMs spectra.The results showed:the dominant contents of lead/cadmium are in residual and ex-changeable speciation,and the exchangeable contents increase greatly after the growth of Calendula officinalis seedlings.It causes negative effects on the height and emergency rate of Calendulaofficinalis seedlings with lead/cadmium,and the Calen-dulaofficinalis is able to positively improve loess characteristics.The root of Calendulaofficinalis appears to be longer,thin-ner and flexuous.The maximum absorption band of DOM in UV spectra locates in 200~240 nm,and the sharper and greater in-tensity of peaks can be detected under lead/cadmium stress.The absorption peaks in FT-IR spectra move from 3 444 and 1 637 to 3 440 and 1 645 cm-1 ,respectively,indicating the combination effects between metal ions and functional groups of—OH and C—O .The dominant fluorescence peaks of DOM are found to be aroundλex/em=240/430,described as fulvic-acid like compo-nents,and metals in loess affect more on peaks intensity than locations.The characteristics of DOM in Rhizosphere loess of Calendula officinalis seedlings contain much micro-eco-environmental information,and the spectral approaches are efficient to reveal the relationship between DOM and chemical speciation of lead/cadmium.%在污染场地植物修复过程中,植物根系通过释放活性分泌物主动

  15. “IN VITRO” MULTIPLICATION OF CALENDULA OFFICINALIS L.

    Directory of Open Access Journals (Sweden)

    Smaranda Vantu

    2015-09-01

    steps: the shoots were excised and transferred to fresh medium and then rooting of these shoots was achieved on the same medium with 0,02 mg/l benzylaminopurine and 1 mg/l 2,4 dichlorophenoxyacetic acid. The excised shoots were subcultured for roots induction. Regenerated plants were transferred to ex vitro conditions for an acclimatisation period.

  16. Mechanical harvesting and cleaning of Calendula officinalis and Dimorphotheca pluvialis

    NARCIS (Netherlands)

    Breemhaar, H.G.; Bouman, A.

    1995-01-01

    From 1990 to 1994 the research institutes of the Agricultural Research Department in the Netherlands (DLO-NL) investigated potential new arable oilseed crops for industrial application as part of the National Oilseeds Programme (NOP). The Institute of Agricultural and Environmental Engineering (IMAG

  17. Effects of Calendula Essential Oil-Based Cream on Biochemical Parameters of Skin of Albino Rats against Ultraviolet B Radiation.

    Science.gov (United States)

    Mishra, Arun K; Mishra, Amrita; Verma, Anurag; Chattopadhyay, Pronobesh

    2012-01-01

    Reactive oxygen species (ROS) generated from UV-B radiation have the capacity to cause oxidative decomposition which leads to the formation of toxic components as well as lipid peroxidation. Considering this fact, the present study was performed to evaluate the effect of a cream (O/W) containing the essential oil of Calendula officinalis on biochemical parameters of the skin of albino rats against UV-B radiation. The fingerprint analysis of Calendula essential oil was performed by HPLC with special reference to 1,8-cineole and α-pinene. The results indicated that the treatment with creams containing 4% and 5% of Calendula essential oil caused a significant decrease in the malonyldialdehyde level, whereas the levels of catalase, glutathione, superoxide dismutase, ascorbic acid, and the total protein level were significantly increased after 1 month of daily irradiation and treatment when compared to untreated control groups. The results suggest that the cutaneous application of the essential oil of Calendula prevents UV-B-induced alterations in the level of antioxidants in skin tissue.

  18. Response of FTIR and Raman Spectra on Cell Wall of Calendula Officinalis Seedlings Roots to the Co-Contamination Stress of Lead and Cadmium in Loess%黄土区金盏菊幼苗根部细胞壁对 Pb/Cd复合胁迫响应的FTIR和Raman光谱

    Institute of Scientific and Technical Information of China (English)

    范春辉; 高雅琳; 杜波

    2016-01-01

    Calendula officinalis seedlings roots to the stress of lead/cadmium in loess .%植物修复法是新兴的重金属污染土壤修复手段,也是未来极富应用潜力的主流技术之一。植物根部细胞壁作为重金属/土壤/植物相的交界面,天然地成为修复效能调控过程的关键部位和信号通道。植物细胞壁与重金属离子的作用行为具有物理化学和生理生化的双重属性,但以光谱技术为切入点,原位解析植物根部细胞壁对土壤重金属离子的响应关系还不多见。以黄土区修复植物金盏菊幼苗为研究对象,分析Pb/Cd复合胁迫对其根部细胞壁形貌的影响,借助X射线荧光光谱(XRF)、X射线衍射(XRD)、傅里叶变换红外光谱(FTIR)和拉曼光谱(Raman)揭示细胞壁对Pb/Cd胁迫的响应信号。结果发现:Pb/Cd胁迫导致金盏菊根部细胞壁弯曲萎缩,表面分布若干点状深色沉积物颗粒;XRF证实细胞壁Pb/Cd含量增加,但XRD图谱没有发现典型Pb/Cd结晶峰。FTIR图谱中—OH振动峰定位于3416cm-1处,表明Pb/Cd离子与—OH间可能存在配位键合;1701和1593cm-1处的特征峰分别移动到1736和1618cm-1,说明Pb/Cd胁迫改变了金盏菊根部细胞壁蛋白质结构属性。Raman光谱中2960cm-1附近峰强增加,暗示Pb/Cd胁迫影响了细胞壁纤维素分子排列方向。可以认为,细胞壁组分(果胶、蛋白质、纤维素等)和典型官能团(—OH,N—H,CO等)对于减缓Pb/Cd胁迫引起的金盏菊根部细胞壁毒害效应贡献较大。

  19. Calendula extract: effects on mechanical parameters of human skin.

    Science.gov (United States)

    Akhtar, Naveed; Zaman, Shahiq Uz; Khan, Barkat Ali; Amir, Muhammad Naeem; Ebrahimzadeh, Muhammad Ali

    2011-01-01

    The aim of this study was to evaluate the effects of newly formulated topical cream of Calendula officinalis extract on the mechanical parameters of the skin by using the cutometer. The Cutometer 580 MPA is a device that is designed to measure the mechanical properties of the skin in response to the application of negative pressure. This non-invasive method can be useful for objective and quantitative investigation of age related changes in skin, skin elasticity, skin fatigue, skin hydration, and evaluation of the effects of cosmetic and antiaging topical products. Two creams (base and formulation) were prepared for the study. Both the creams were applied to the cheeks of 21 healthy human volunteers for a period of eight weeks. Every individual was asked to come on week 1, 2, 3, 4, 5, 6, 7, and 8 and measurements were taken by using Cutometer MPA 580 every week. Different mechanical parameters of the skin measured by the cutometer were; R0, R1, R2, R5, R6, R7, and R8. These were then evaluated statistically to measure the effects produced by these creams. Using ANOVA, and t-test it was found that R0, and R6 were significant (p 0.05). The instrumental measurements produced by formulation reflected significant improvements in hydration and firmness of skin.

  20. Rapid evaluation and comparison of natural products and antioxidant activity in calendula, feverfew, and German chamomile extracts.

    Science.gov (United States)

    Agatonovic-Kustrin, Snezana; Babazadeh Ortakand, Davoud; Morton, David W; Yusof, Ahmad P

    2015-03-13

    The present study describes a simple high performance thin layer chromatographic (HPTLC) method for the simultaneous quantification of apigenin, chamazulene, bisabolol and the use of DPPH free radical as a post-chromatographic derivatization agent to compare the free radical scavenging activities of these components in leaf and flower head extracts from feverfew, German chamomile and marigold from the Asteraceae family. Feverfew (Tanacetum parthenium) leaves have been traditionally used in the treatment of migraine with parthenolide being the main bioactive compound. However, due to similar flowers, feverfew is sometimes mistaken for the German chamomile (Matricaria recutita). Bisabolol and chamazulene are the main components in chamomile essential oil. Marigold (Calendula officinalis) was included in the study for comparison, as it belongs to the same family. Parthenolide was found to be present in all leaf extracts but was not detected in calendula flower extract. Chamazulene and bisabolol were found to be present in higher concentrations in chamomile and Calendula flowers. Apigenin was detected and quantified only in chamomile extracts (highest concentration in flower head extracts). Antioxidant activity in sample extracts was compared by superimposing the chromatograms obtained after post-chromatographic derivatization with DPPH and post-chromatographic derivatization with anisaldehyde. It was found that extracts from chamomile flower heads and leaves have the most prominent antioxidant activity, with bisabolol and chamazulene being the most effective antioxidants.

  1. LIN Qiu-cheng (

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Professor LIN Qiu-cheng, born in Xianyou county, Fujian Province in March 1931, was graduated from Fujian Medical College in 1954 and assigned to Union Hospital affiliated to Fujian Medical College. From 1958 to 1961, he studied traditional Chinese medicine (TCM) systematically and was awarded the third class prize by State Health Ministry for his excellent score. After then, he assumed office as vice president of Fujian Research Institute of TCM. Also he had been the vice president of Fujian People's Hospital and Fujian Second People's Hospital. Now, Prof. LIN is chief physician of Fujian Research Institute of TCM, tutor of postgraduates on integrated traditional Chinese and western medicine (TCM-WM) of Fujian College of TCM, supervisor of Ph.D. students in Beijing University of TCM, evaluation expert of State Drug Administration, member of State Drug Committee of Clinical Research Base, director of Committee on Medical Ethics and director of Research Department on Gerontology of Fujian Research Institute of TCM.……

  2. Inibição do crescimento micelial de Cercospora calendulae Sacc. por extratos de plantas medicinais

    Directory of Open Access Journals (Sweden)

    J.M. Nascimento

    2013-01-01

    Full Text Available O uso de extratos e óleos essenciais de plantas medicinais tem sido amplamente estudado no controle de doenças de plantas. O objetivo da realização do presente trabalho foi avaliar o efeito de extratos de Ruta graveolens L., Mentha x villosa, Calendula officinalis L., Momordica charantia L., Symphytum officinale L., Ageratum conyzoides L. e Ricinus comunis L., nas concentrações de 0, 250, 500, 1000, 2000, 5000 e 10000 mg L-1, sobre a inibição do crescimento micelial de Cercospora calendulae Sacc. in vitro. Os extratos foram obtidos por infusão. O experimento foi desenvolvido no Laboratório de Fitopatologia da FCA/UFGD, estado de Mato Grosso do Sul, em delineamento inteiramente casualizado, esquema fatorial 7 extratos x 7 concentrações, com seis repetições. Foi detectado efeito dos extratos e suas concentrações sobre o crescimento do fungo, sendo a interação significativa. Os extratos de calêndula, arruda, hortelã e melão de São Caetano, nas maiores concentrações resultaram em maiores porcentagens de inibição, próximas de 100%, 30%, 35% e 40%, respectivamente, a 10000 mg L-1.

  3. Salvia officinalis in dentistry

    Directory of Open Access Journals (Sweden)

    Nikhita Narayanan

    2015-01-01

    Full Text Available Salvia officinalis is a medicinal herb used as an cosmetic, flavoring agent, It has antibacterial, antifungal, anticaries, antiplaque, antiviral, astringent, and other useful properties, it is also used in dental practice for the management of periodontal disease and to prevent halitosis. The objective of this article is to highlight various uses of S. officinalis in the dental field along with its use in medical problems.

  4. Estimativa do filocrono em calêndula Estimating the phyllochron in calendula

    Directory of Open Access Journals (Sweden)

    Jana Koefender

    2008-08-01

    Full Text Available O objetivo deste trabalho foi estimar o filocrono em plantas de calêndula (Calendula officinalis L. cultivadas em diferentes épocas de semeaduras. Realizaram-se três épocas de semeaduras: 06/04/2005, 23/06/2005 e 03/10/2006, no interior de uma estufa plástica com área de 240m2, instalada no campus da Universidade Federal de Santa Maria, UFSM. A semeadura foi realizada em vasos plásticos com capacidade volumétrica para cinco litros. O delineamento experimental foi inteiramente casualizado com 320 vasos em cada época, sendo distribuído em quatro fileiras de 80 vasos cada. Em uma planta de oito vasos de cada quatro fileiras, fez-se a contagem, semanalmente, do número de folhas emergidas da haste principal e na primeira haste lateral. Os dados do número de folhas foram correlacionados com a soma térmica diária acumulada, considerando-se uma temperatura base de 8°C. Constatou-se que o filocrono variou com a época de semeadura, sendo o menor valor igual 15,9°C dia folha-1, obtido na 2ª época de semeadura e o maior, igual a 24,5°C dia folha-1, na 1ª época na haste principal e 48,9°C dia folha-1 na semeadura de outubro na primeira haste lateral.The objective of this study was to estimate the phyllochron in calendula (Calendula officinalis L. grown in different sowing dates. Three sowing dates (06/04/2005, 23/06/2005 and 03/10/2006 were performed inside a 240m2 plastic greenhouse at the Campus of the Federal University of Santa Maria, RS, Brazil. Sowing was in five liter plastic pots. The experimental design was a completely randomized with 320 pots on each sowing date and rows with 80 pots. The number of leaves in the main stem and oi the first lateral branch was counted on a weekly basis in one plant of eight pots. The number of leaves data was regressed against accumulated thermal time, assuming a base temperature of 8oC. The main stem phyllochron varied with sowing date, with the lowest value (15.9°C day leaf -1 obtained on

  5. Rosmarinus officinalis vitroculture initiation

    Directory of Open Access Journals (Sweden)

    Liviu POP

    2007-05-01

    Full Text Available In this experiment we have initiated a Rosmarinus officinalis vitroculture, on different growth media. As biological material we used apexes, taken from an only plant. The development medium have consisted in Murashige and Skoog standard mixture, where growth regulators were added, resulting 4 experimental variants: V0 – control variant – basic medium (BM, V1 – BM + 2mg/l BA + 1mg/l IBA, V2 - BM + 2mg/l BA + 1mg/l IAA, V3 –BM + 2mg/l BA + 1mg/l NAA. The experiment lasted for 90 days. We have found that the initiation of Rosmarinus officinalis vitroculture is possible, the best growth medium for this purpose being the basic one (V0 - Murashige and Skoog without growth regulators.

  6. Effect of biostimulant application on production and flavonoid content of marigold (Calendula officinalis L.

    Directory of Open Access Journals (Sweden)

    Vivian Pupo de Oliveira Machado

    2014-12-01

    Full Text Available The production of medicinal plants as raw material for industry must associate quality with biomass formation and, with this purpose, the application of plant growth regulators has been studied in these crops. The objective of this study was to evaluate the effect of a biostimulant on growth, inflorescence production and flavonoid content in marigold. The experiment was conducted in a greenhouse and the treatments consisted of increasing doses of the biostimulant (0, 3, 6, 9, 12 and 15 mL L-1 applied by foliar spraying in ten consecutive applications. The experiment was arranged in a completely randomized design, with six treatments and ten repetitions. The number of leaves and flowerheads and dry matter of roots increased linearly with increasing doses of the growth promoter, with 20%, 36.97% and 97.28% increases, respectively, compared with the control. The total dry mass and shoot dry mass showed maximum values at the highest dose tested of 15 mL L-1 (with increases of 40.09% and 46.30%, respectively. Plant height and flavonoid content reached the highest values at a dose of 6 mL L-1. The biostimulant promoted the development of marigold and positively influenced the synthesis of the secondary compound of medicinal interest. Among the tested doses, the application of rates between 6 and 9 mL L-1 of the biostimulant is recommended for more efficient large-scale production of marigold.

  7. Developements of Calendula officinalis Oil%金盏菊油开发利用

    Institute of Scientific and Technical Information of China (English)

    郭玉宝; 裘爱泳; 徐霞

    2002-01-01

    金盏菊富含多种功能性化合物,如类黄酮、三萜烯皂甙、类胡萝卜素、维生素E及共轭十八碳三烯酸等.本文主要综述国内外对金盏菊油研究现状和可能的工业应用,并展望其开发前景.

  8. Determination of total lipids and characterization of marigold flower extracts (Calendula officinalis

    Directory of Open Access Journals (Sweden)

    Novković Vesna M.

    2005-01-01

    Full Text Available Bioactive extracts from marigold flower are important ingredients for parapharmaceutical and cosmetic preparations. Their antiflogistic holeretic.antimicrobic, antidermatic and anticancer effects are due to the presence of flavonoids, carotenoids, etheric oils, and terpenoids. This study presents the results of spectrophotometric investigation for the overall carotene content calculated as (3-caroten (442 nm, visual and physico-chemical characteristics according to Ph.Jug. V in oil extracts of marigold flower obtained by maceration (room temperature and 70°C and percolation (room temperature with olive oil and sunflower oil by original procedures.The largest content of (3-carotene (57.5 mg/kg of oil extracts is in the oil extract obtained by maceration for 72 hours with olive oil (solvomodulus 1:5 m/m at room temperature.

  9. LINS Curve in Romanian Economy

    Directory of Open Access Journals (Sweden)

    Emilian Dobrescu

    2016-02-01

    Full Text Available The paper presents theoretical considerations and empirical evidence to test the validity of the Laffer in Narrower Sense (LINS curve as a parabola with a maximum. Attention is focused on the so-called legal-effective tax gap (letg. The econometric application is based on statistical data (1990-2013 for Romania as an emerging European economy. Three cointegrating regressions (fully modified least squares, canonical cointegrating regression and dynamic least squares and three algorithms, which are based on instrumental variables (two-stage least squares, generalized method of moments, and limited information maximum likelihood, are involved.

  10. 金盏菊花类胡萝卜素的提取及性质研究%Study on the Extraction and Property of Carotenoids from Colendula Officinalis L.Flowers

    Institute of Scientific and Technical Information of China (English)

    胡晓丹; 谢笔钧

    2001-01-01

    主要对中国产金盏菊花中类胡萝卜素的提取、纯化及其稳定性进行了研究,为金盏菊花类胡萝卜素的提取和应用提供了科学依据。%The paper mainly studied the extraction, purification, stability of carotenoids from calendula officinalis L. Flowers in China, which provides scientfic basis for the extraction and application of the carotenoids.

  11. LIN-44/Wnt directs dendrite outgrowth through LIN-17/Frizzled in C. elegans Neurons.

    Directory of Open Access Journals (Sweden)

    Leonie Kirszenblat

    2011-09-01

    Full Text Available Nervous system function requires proper development of two functional and morphological domains of neurons, axons and dendrites. Although both these domains are equally important for signal transmission, our understanding of dendrite development remains relatively poor. Here, we show that in C. elegans the Wnt ligand, LIN-44, and its Frizzled receptor, LIN-17, regulate dendrite development of the PQR oxygen sensory neuron. In lin-44 and lin-17 mutants, PQR dendrites fail to form, display stunted growth, or are misrouted. Manipulation of temporal and spatial expression of LIN-44, combined with cell-ablation experiments, indicates that this molecule is patterned during embryogenesis and acts as an attractive cue to define the site from which the dendrite emerges. Genetic interaction between lin-44 and lin-17 suggests that the LIN-44 signal is transmitted through the LIN-17 receptor, which acts cell autonomously in PQR. Furthermore, we provide evidence that LIN-17 interacts with another Wnt molecule, EGL-20, and functions in parallel to MIG-1/Frizzled in this process. Taken together, our results reveal a crucial role for Wnt and Frizzled molecules in regulating dendrite development in vivo.

  12. Saponaria officinalis Seeds Germination Morphology and Biology

    Directory of Open Access Journals (Sweden)

    Margaret Yu. Ishmuratova

    2013-01-01

    Full Text Available This work deals with the study of influence of terms and storage conditions on Saponaria officinalis seed quality, stored in different containers (paper, plastic, fabric and glass, in the different temperature conditions, under light or darkness. The morphology, biology of Saponaria officinalis seeds was described. Basing in the obtained results, we recommend to store Saponaria officinalis seeds within 3 months in paper container at the temperature of - 18˚С.

  13. Rosmarinus officinalis L.

    OpenAIRE

    2015-01-01

    Проведено изучение аминокислотного состава побегов пряно-ароматического растения – розмарина лекарственного (Rosmarinus officinalis L.), интродуцированного в ботаническом саду Пятигорского медико-фармацевтического института. Экспериментальные исследования проводили на аминокислотном анализаторе – ААА 400, автоматизированном жидкостном хроматографе с компьютерным управлением. Установлено наличие 16 аминокислот, в том числе 9 незаменимых. В наибольшем количестве содержатся аспарагиновая кислота...

  14. Dr. Lin Zhu's Clinical Experience in Treating Mental Disorders

    Institute of Scientific and Technical Information of China (English)

    Ma Xueqing; Wang Xinzhong

    2005-01-01

    @@ Mental disorders are mainly caused by the emotional factors. Chief physician Lin Zhu, a famous TCM doctor in Beijing, is very good at treating this kind of disorders. The following is a summary of Prof. Lin's experience in this aspect.

  15. Evaluation of Calendula mucilage as a mucoadhesive and controlled release component in buccal tablets.

    Science.gov (United States)

    Sabale, V; Patel, V; Paranjape, A

    2014-01-01

    Mucoadhesive drug delivery systems were developed to sustain drug delivery via various mucus membranes for either local or systemic delivery of poorly absorbed drugs such as peptides and proteins as well as drugs that are subjected to high first-pass metabolism. The present study was undertaken to use isolated Calendula mucilage as a mucoadhesive agent and to formulate controlled release buccoadhesive tablets with an intention to avoid hepatic first-pass metabolism as well as to enhance residence time of drug in the buccal cavity. The mucilage was isolated from the Calendula petals by aqueous extraction method and characterized for various physiochemical parameters as well as for its adhesive properties. By using direct compression technique, tablets were prepared containing dried mucilage and chlorpheniramine maleate (CPM) as a model drug. Three batches of tablets were prepared and evaluated containing three mucoadhesive components namely Methocel K4M, Carbopol 974P and isolated Calendula mucilage in 16.66%, 33.33 % and 50 % (1:2:3 ratio) resulting in 9 different formulations. FTIR studies between mucilage and CPM suggested the absence of a chemical interaction between CPM and Calendula mucilage. The results of the study showed that the isolated mucilage had good physicochemical and morphological characteristics and tablets conformed to the pharmacopoeial specifications. Also in vitro release studies showed controlled action of drug with increasing the concentration of the isolated Calendula mucilage as a mucoadhesive agent in the formulations. Permeability studies indicated that permeability behavior was not statistically different (P>0.05) by changing the mucoadhesive component. The formulated mucoadhesive tablets for buccal administration containing 75 mg Calendula mucilage showed controlled drug release. Thus, mucoadhesive natural Calendula mucilage based buccal tablets for controlled release were successfully formulated.

  16. Lin Hairong:A Quiet Commentary

    Institute of Scientific and Technical Information of China (English)

    Jade; Franklin

    2007-01-01

    In looking at the works of Lin Hairong, one senses that the artist is seeking to capture life's tranquil moments; those fleeting glimpses of halcyon clarity and peace that remain uncontaminated by the frenetic pace of daily existence. Moments such as these have become few and far between however, in an age that the artist feels "is too quick to change".

  17. Analysis list: lin-42 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-42 + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-42.1.tsv h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-42.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/li...n-42.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-42..tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/.gml ...

  18. The Lin28/let-7 Axis Regulates Glucose Metabolism

    NARCIS (Netherlands)

    Zhu, Hao; Shyh-Chang, Ng; Segre, Ayellet V.; Shinoda, Gen; Shah, Samar P.; Einhorn, William S.; Takeuchi, Ayumu; Engreitz, Jesse M.; Hagan, John P.; Kharas, Michael G.; Urbach, Achia; Thornton, James E.; Triboulet, Robinson; Gregory, Richard I.; Altshuler, David; Daley, George Q.

    2011-01-01

    The let-7 tumor suppressor microRNAs are known for their regulation of oncogenes, while the RNA-binding proteins Lin28a/b promote malignancy by inhibiting let-7 biogenesis. We have uncovered unexpected roles for the Lin28/let-7 pathway in regulating-metabolism. When overexpressed in mice, both Lin28

  19. Analysis list: lin-37 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-37 Embryo,Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/li...n-37.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-37.5.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/ce10/target/lin-37.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-37.Embryo....tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-37.Larvae.tsv http:

  20. Analysis list: lin-15B [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-15B Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-15...B.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-15B.5.tsv http://dbarchive.biosciencedbc....jp/kyushu-u/ce10/target/lin-15B.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-15B.Larvae.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  1. Complete genome sequence of Bacillus amyloliquefaciens strain Co1-6, a plant growth-promoting rhizobacterium of Calendula officinalis

    Energy Technology Data Exchange (ETDEWEB)

    Koeberl, Martina; White, Richard A.; Erschen, Sabine; Spanberger, Nora; El-Arabi, Tarek F.; Jansson, Janet K.; Berg, Gabriele

    2015-08-13

    The genome sequence of Bacillus amyloliquefaciens strain Co1-6, a plant growth-promoting rhizobacterium (PGPR) with broad-spectrum antagonistic activities against plant pathogenic fungi, bacteria and nematodes, consists of a single 3.9 Mb circular chromosome. The genome reveals genes putatively responsible for its promising biocontrol and PGP properties.

  2. 金盏菊色素的提取及分离%Calendula officinalis Pigment Extraction and Separation

    Institute of Scientific and Technical Information of China (English)

    马丽英; 彭军强

    2013-01-01

    采用纸层析、硅胶板层析和柱层析分别对水和酒精提取的金盏菊色素进行分离.结果表明:水提取的色素较为纯净,只有一种黄色色素;而酒精可将金盏菊中的类胡萝卜素提取出来.水提取的色素不稳定,易被光分解;而酒精提取的色素光稳定性较好.酒精提取的色素用展开剂苯∶乙醚∶丙酮=1∶1∶1及丙酮∶乙醚∶氯仿=1∶1∶1分离效果较好.

  3. Stability study of calendula officinalis yellow pigment%金盏菊桔费色素稳定性研究

    Institute of Scientific and Technical Information of China (English)

    李清芳; 马成仓; 濮云飞

    2001-01-01

    研究了金盏菊桔黄色素的溶解性,对光、热的稳定性及几种常用食品添加剂对金盏菊桔黄色素稳定性的影响,并分析了酸、碱、几种常见离子对其稳定性的作用,为生产上使用色素提供依据.

  4. 金盏花配方施肥效应研究%Studies on Formula Fertilization Effect to Calendula officinalis

    Institute of Scientific and Technical Information of China (English)

    朱亚; 赵永平; 何庆祥; 周彦芳; 刘强

    2011-01-01

    采用"3414"试验设计,对氮磷钾肥不同配施比例与金盏花产量关系进行研究.结果表明:不同氮、磷、钾肥配拖对鲜花产量影响达显著水平,各施肥处理的鲜花产量分别比不施肥处理增产3 045.9-6 414.2kg/hm2;缺氮对产量影响最大,磷肥次之,钾肥最小;最佳产量施肥量为:N 158.54 kg/hm2,P2O5 126.61kg/hm2,K2O 62.80kg/hm2,最佳产量为33 722.58 kg/hm2.

  5. HPLC Fingerprint of Calendula officinalis Flower%金盏花HPLC特征指纹图谱研究

    Institute of Scientific and Technical Information of China (English)

    邢占芬; 成洪达; 张平平; 宫磊; 马丽娅

    2014-01-01

    目的:建立金盏花的HPLC特征指纹图谱,为科学评价其质量提供方法.方法:以Hypersil ODS C18柱(250 mm×4.6 mm,5μm)为色谱柱,乙腈-水为流动相进行梯度洗脱,流速1.0 mL/min,检测波长220 nm,柱温35cc;采用中药色谱指纹图谱评价系统进行相似度分析.结果:确定了含有11个色谱峰的特征指纹图谱,不同产地金盏花特征图谱相似度均在0.90以上.结论:建立的方法简单、可靠,可用于金盏花的鉴别和质量控制.

  6. Effect of Calendula officinalis hydroalcoholic extract on passive avoidance learning and memory in streptozotocin-induced diabetic rats

    Directory of Open Access Journals (Sweden)

    Shirin Moradkhani

    2015-01-01

    Conclusion: Taken together, CO extract can improve PAL and memory impairments in STZ-diabetic rats. This improvement may be due to its antioxidant, anticholinergic activities or its power to reduce hyperglycemia.

  7. The seamy side of natural medicines: contact sensitization to arnica (Arnica montana L.) and marigold (Calendula officinalis L.).

    Science.gov (United States)

    Reider, N; Komericki, P; Hausen, B M; Fritsch, P; Aberer, W

    2001-11-01

    Medical remedies of plant origin have gained increasing popularity in recent years. Both anaphylactic and eczematous allergic reactions are on the rise, accordingly. Arnica and marigold, both of the Compositae family, are in widespread use, but only limited data are available on their allergenic potential. We tested 443 consecutive patients, in addition to the European standard and other series, with Compositae mix, sesquiterpene lactone mix, arnica, marigold, and propolis. 5 subjects ( approximately 1.13%) reacted to arnica, 9 ( approximately 2.03%) to marigold. The Compositae mix was positive in 18 cases ( approximately 4.06%). Among them were 3 out of 5 individuals with a sensitization to arnica, and 4 out of 9 who reacted to marigold. Sensitization to arnica and marigold was often accompanied by reactions to nickel, Myroxylon Pereirae resin, fragrance mix, propolis, and colophonium. We conclude that Compositae allergy contributes significantly to the epidemiology of contact dermatitis and that sensitization to arnica and marigold cannot be assessed by testing with the Compositae or sesquiterpene mix alone. As extracts of these plants are frequently used in occupational and cosmetic products, patch testing with additional plant extracts or adjustment of the commercial Compositae mix to regional conditions is recommended.

  8. The LIN-15A and LIN-56 transcriptional regulators interact to negatively regulate EGF/Ras signaling in Caenorhabditis elegans vulval cell-fate determination.

    Science.gov (United States)

    Davison, Ewa M; Saffer, Adam M; Huang, Linda S; DeModena, John; Sternberg, Paul W; Horvitz, H Robert

    2011-03-01

    The restricted expression of epidermal growth factor (EGF) family ligands is important for proper development and for preventing cancerous growth in mammals. In Caenorhabditis elegans, the class A and B synthetic multivulva (synMuv) genes redundantly repress expression of lin-3 EGF to negatively regulate Ras-mediated vulval development. The class B synMuv genes encode proteins homologous to components of the NuRD and Myb-MuvB/dREAM transcriptional repressor complexes, indicating that they likely silence lin-3 EGF through chromatin remodeling. The two class A synMuv genes cloned thus far, lin-8 and lin-15A, both encode novel proteins. The LIN-8 protein is nuclear. We have characterized the class A synMuv gene lin-56 and found it to encode a novel protein that shares a THAP-like C(2)CH motif with LIN-15A. Both the LIN-56 and LIN-15A proteins localize to nuclei. Wild-type levels of LIN-56 require LIN-15A, and wild-type levels and/or localization of LIN-15A requires LIN-56. Furthermore, LIN-56 and LIN-15A interact in the yeast two-hybrid system. We propose that LIN-56 and LIN-15A associate in a nuclear complex that inhibits vulval specification by repressing lin-3 EGF expression.

  9. The reproductive strategies of the heterocarpic annual Calendula arvensis (Asteraceae)

    Science.gov (United States)

    Ruiz De Clavijo, E.

    2005-09-01

    Achene polymorphism and various aspects of the reproductive biology of the annual Calendula arvensis L. (Field marigold), were studied to determine the reproductive strategies of the plant. This species normally produces three types of achene: rostrate, cymbiform and annular. Rostrate and cymbiform achenes are larger and heavier than annular achenes, and are adapted to long-range dispersal (by epizoochory and anemochory, respectively). In contrast, annular achenes are smaller in size and weight, and are adapted to short-range dispersal. Achenes germinate over a broad range of temperatures (both in light and in darkness), exhibiting cymbiform achenes the highest germination percentages and annular achenes the lowest under all conditions tested. A fraction of the three types of achenes exhibit dormancy and presumably enter the soil seedbank. Achene types adapted for long-range dispersal (rostrate and cymbiform achenes) produce seedlings that are best able to emerge from deeper burial depths, and that are initially stronger and exhibit earlier flowering than the plants from the annular achenes (which are likely to disperse over shorter distances). These features, together with the fact that fruiting occurs even in the absence of pollinators (automatic geitonogamy), the different mechanisms for achene dispersal (zoochory, anemochory and myrmerochory), and the extended germination, flowering and fruiting periods, facilitate establishment and expansion of this species in unpredictable and disturbed habitats.

  10. Analysis list: lin-13 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-13 Embryo,Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/...lin-13.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-13.5.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/ce10/target/lin-13.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-13.Embryo....tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-13.Larvae.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/ce10/colo/Embryo.gml,http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  11. Analysis list: lin-35 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-35 Adult,Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-35.1.tsv http:...//dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-35.5.tsv http://dbarchive.bioscienced...bc.jp/kyushu-u/ce10/target/lin-35.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-35.Adult.tsv,http:...//dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-35.Larvae.tsv http://...dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Adult.gml,http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  12. Analysis list: lin-53 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-53 Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-53....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-53.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/li...n-53.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-53.Larvae.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  13. Analysis list: lin-61 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-61 Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-61....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-61.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/li...n-61.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-61.Larvae.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  14. Analysis list: lin-39 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-39 Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-39....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-39.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/li...n-39.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-39.Larvae.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  15. Analysis list: lin-11 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-11 Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-11....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-11.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/li...n-11.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-11.Larvae.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  16. Analysis list: lin-52 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-52 Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-52....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-52.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/li...n-52.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-52.Larvae.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  17. Analysis list: lin-9 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-9 Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-9.1....tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-9.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/li...n-9.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-9.Larvae.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  18. Analysis list: lin-54 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-54 Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-54....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-54.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/li...n-54.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-54.Larvae.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  19. Calendula oil processing : seed classification, oil extraction, refining process development and oil quality aspects

    NARCIS (Netherlands)

    Janssens, R.J.J.

    2000-01-01

    The difference in Calendula oil quality from fractions obtained after seed classification is enormous. The oil quality varies from excellent to very poor, according to important aspects such as in the hulls and dust fraction, high free fatty acid values (13% vs. 0.6%) are found. This can be explaine

  20. Anticandidal, antibacterial, cytotoxic and antioxidant activities of Calendula arvensis flowers.

    Science.gov (United States)

    Abudunia, A-M; Marmouzi, I; Faouzi, M E A; Ramli, Y; Taoufik, J; El Madani, N; Essassi, E M; Salama, A; Khedid, K; Ansar, M; Ibrahimi, A

    2016-12-20

    Calendula arvensis (CA) is one of the important plants used in traditional medicine in Morocco, due to its interesting chemical composition. The present study aimed to determine the anticandidal, antioxidant and antibacterial activities, and the effects of extracts of CA flowers on the growth of myeloid cancer cells. Also, to characterize the chemical composition of the plant. Flowers of CA were collected based on ethnopharmacological information from the villages around the region Rabat-Khemisset, Moroccco. The hexane and methanol extracts were obtained by soxhlet extraction, while aqueous extracts was obtained by maceration in cold water. CA extracts were assessed for antioxidant activity using four different methods (DPPH, FRAP, TEAC, β-carotene bleaching test). Furthermore, the phenolic and flavonoid contents were measured, also the antimicrobial activity has been evaluated by the well diffusion method using several bacterial and fungal strains. Finally, extracts cytotoxicity was assessed using MTT test. Phytochemical quantification of the methanolic and aqueous extracts revealed that they were rich with flavonoid and phenolic content and were found to possess considerable antioxidant activities. MIC values of methanolic extracts were 12.5-25μg/mL. While MIC values of hexanolic extracts were between 6.25-12.5μg/mL and were bacteriostatic for all bacteria while methanolic and aqueous extracts were bactericidal. In addition, the extracts exhibited no activity on Candida species except the methanolic extract, which showed antifungal activity onCandida tropicalis 1 and Candida famata 1. The methanolic and aqueous extracts also exhibited antimyeloid cancer activity (IC50 of 31μg/mL). In our study, we conclude that the methanolic and aqueous extracts were a promising source of antioxidant, antimicrobial and cytotoxic agents.

  1. C. elegans LIN-18 is a Ryk ortholog and functions in parallel to LIN-17/Frizzled in Wnt signaling.

    Science.gov (United States)

    Inoue, Takao; Oz, Helieh S; Wiland, Debra; Gharib, Shahla; Deshpande, Rashmi; Hill, Russell J; Katz, Wendy S; Sternberg, Paul W

    2004-09-17

    Wnt proteins are intercellular signals that regulate various aspects of animal development. In Caenorhabditis elegans, mutations in lin-17, a Frizzled-class Wnt receptor, and in lin-18 affect cell fate patterning in the P7.p vulval lineage. We found that lin-18 encodes a member of the Ryk/Derailed family of tyrosine kinase-related receptors, recently found to function as Wnt receptors. Members of this family have nonactive kinase domains. The LIN-18 kinase domain is dispensable for LIN-18 function, while the Wnt binding WIF domain is required. We also found that Wnt proteins LIN-44, MOM-2, and CWN-2 redundantly regulate P7.p patterning. Genetic interactions indicate that LIN-17 and LIN-18 function independently of each other in parallel pathways, and different ligands display different receptor specificities. Thus, two independent Wnt signaling pathways, one employing a Ryk receptor and the other a Frizzled receptor, function in parallel to regulate cell fate patterning in the C. elegans vulva.

  2. RNA-binding protein Lin28 in cancer and immunity.

    Science.gov (United States)

    Jiang, Shuai; Baltimore, David

    2016-05-28

    The highly conserved RNA-binding protein, Lin28, is involved in many biological processes, including development, reprogramming, pluripotency, and metabolism. Importantly, Lin28 functions as an oncogene, promoting tumor progression and metastasis in various human cancers. Lin28 can regulate gene expression either by directly binding to mRNAs or by blocking microRNA biogenesis, and the underlying mechanisms include Let-7-dependent and Let-7-independent modes of action. Recent evidence shows that Lin28 also plays a fundamental role in immunity. The roles of Lin28 in disease are complex and require characterization of its physiological functions in cancer and immunological contexts. Here we review emerging information on the role of Lin28 in cancer and immunity and the molecular mechanisms it uses. We discuss our present knowledge of the system and highlight remaining mysteries related to the functions of this small RNA-binding protein. This knowledge may lead to Lin28 becoming a diagnostic marker for cancer or immune-related diseases and a possible therapeutic target.

  3. PHYTOCHEMICAL ANALYSIS OF FUMARIA OFFICINALIS L. (FUMARIACEAE)

    OpenAIRE

    Paltinean, Ramona; Toiu, Anca; Wauters, Jean-Noël; Frederich, Michel; Tits, Monique; Angenot, Luc; Mircea TAMAS; Crisan, Gianina

    2016-01-01

    The present study describes the investigation of active compounds from several samples of Fumaria officinalis L. (Fumariaceae). The identification of the isoquinoline alkaloids (allocryptopine, chelidonine, protopine, bicuculline, sanguinarine, cheleritrine, stylopine and hydrastine) was performed by comparison with reference standards using an HPLC-DAD method, and their quantification by LC-DAD and spectrophotometric methods. The presence of polyphenolic compounds was simultaneously assessed...

  4. Development of mouthwash with Rosmarinus officinalis extract

    Directory of Open Access Journals (Sweden)

    Isabela Moreira Baumgratz de Paula

    2014-12-01

    Full Text Available Rosmarinus officinalis, which belongs to the Lamiaceaefamily, is a species of medicinal flora with therapeutic properties. In order to exploit the benefits of these properties, a mouthwash formulation was developed, with careful selection of raw materials to meet pharmacotechnical requirements. Extracts of the plant were incorporated into a mouthwash, which was shown to have inhibitory action in vitro against the micro-organisms commonly found in periodontics. Controls for assessing the quality of the drugs were carried out, quantifying phenols and flavonoids as chemical markers. Mouthwash solutions were formulated containing 0.1, 5 and 10% ethanol extract of R. officinalis; and 0.05, 5 and 10% of the hexane fraction of R. officinalis. In order to evaluate synergism, ethanol extract and hexane fraction were also added to formulations containing 0.05% sodium fluoride and 0.12% chlorhexidine digluconate. These formulations were assessed for inhibitory effect against the specific microorganisms involved in the process of bacterial plaque formation, S. mutans(ATCC25175 and C. albicans(ATCC 10231, frequently found in cases of oral infections. The agar diffusion method was used to evaluate the inhibitory activity of extracts and formulations. All mouthwash solutions displayed inhibitory activity having higher sensitivity to S. mutansfor the 5% ethanol extract+0.05% sodium fluoride, and greater sensitivity to C. albicansfor the 10% hexane fraction. Results were characterized by the appearance of a growth inhibition halo, justifying the utilization and association of extracts of R. officinalis.

  5. Phytoremediation of stable Cs from solutions by Calendula alata, Amaranthus chlorostachys and Chenopodium album.

    Science.gov (United States)

    Moogouei, Roxana; Borghei, Mehdi; Arjmandi, Reza

    2011-10-01

    Uptake rate of (133)Cs, at three different concentrations of CsCl, by Calendula alata, Amaranthus chlorostachys and Chenopodium album plants grown outdoors was studied. These plants grow abundantly in semi-arid regions and their varieties exist in many parts of the world. When exposed to lowest Cs concentration 68 percent Cs was remediated by Chenopodium album.(133)Cs accumulation in shoots of Amaranthus chlorostachys reached its highest value of 2146.2 mg kg(-1) at a (133)Cs supply level of 3.95 mg l(-1) of feed solution. The highest concentration ratio value was 4.89 for Amaranthus chlorostachys, whereas for the other tests it ranged from 0.74 to 3.33. Furthermore uptake of (133)Cs by all three species increased with increasing metal concentrations. The results also indicated that hydroponically grown Calendula alata, Amaranthus chlorostachys and Chenopodium album could be used as potential candidate plants for phytoremediation of solutions contaminated with Cs.

  6. On the low regularity of the Benney-Lin equation

    Science.gov (United States)

    Chen, Wengu; Li, Junfeng

    2008-03-01

    We consider the low regularity of the Benney-Lin equation ut+uux+uxxx+[beta](uxx+uxxxx)+[eta]uxxxxx=0. We established the global well posedness for the initial value problem of Benney-Lin equation in the Sobolev spaces for 0[greater-or-equal, slanted]s>-2, improving the well-posedness result of Biagioni and Linares [H.A. Biaginoi, F. Linares, On the Benney-Lin and Kawahara equation, J. Math. Anal. Appl. 211 (1997) 131-152]. For s<-2 we also prove some ill-posedness issues.

  7. Effects of chlorhexidine, essential oils and herbal medicines (Salvia, Chamomile, Calendula) on human fibroblast in vitro.

    Science.gov (United States)

    Wyganowska-Swiatkowska, Marzena; Urbaniak, Paulina; Szkaradkiewicz, Anna; Jankun, Jerzy; Kotwicka, Malgorzata

    2016-01-01

    Antiseptic rinses have been successfully used in inflammatory states of the gums and oral cavity mucosa. Antibacterial effects of chlorhexidine, essential oils and some herbs are well documented. Reaction of host tissue to these substances has much poorer documentation. The aim of the study was to analyse the influence of chlorhexidine (CHX), essential oil (EO: thymol, 0.064%; eucalyptol, 0.092%; methyl salicylate, 0.060%; menthol, 0.042%) mouth rinses and salvia, chamomile and calendula brews on fibroblast biology in vitro. The human fibroblast CCD16 line cells were cultured in incubation media which contained the examined substances. After 24 and 48 hours, the cell morphology, relative growth and apoptosis were evaluated. Exposure of fibroblasts to CHX, EO or salvia caused various changes in cell morphology. Cells cultured for 48 hours with CHX revealed a noticeably elongated shape of while cells cultured in high EO concentration or with salvia were considerably smaller and contracted with fewer projections. Chlorhexidine, EO and salvia reduced the fibroblast proliferation rate and stimulated cell death. Both reactions to EO were dose dependent. Cells exposure to chamomile or calendula brews did not change morphology or proliferation of fibroblasts. The results of this in vitro study showed that in contrast to chamomile and calendula, the brews of EO, CHX or salvia had a negative influence on fibroblast biology.

  8. Comparison of the Effects of Chamomile and Calendula Ointments on Diaper Rash

    Directory of Open Access Journals (Sweden)

    Zahra Afshari

    2015-06-01

    Full Text Available Background: Diaper Rash is a common skin disorder in the infancy that makes infants and their parents anxious and nervous. Due to the high prevalence of dermatitis and its complications in addition to adverse effects of chemical drugs, herbal therapy is an appropriate solution. Aim: Comparison of the effects of Chamomile and Calendula ointments on severity of Diaper Rash. Methods: This double-blind randomized controlled trial was conducted on 90 infants under one year who were hospitalized in Sabalan Hospital of Ardebil. The infants were treated by Chamomile and Calendula ointments for one week. The severity of dermatitis was evaluated on days one, 3 and 7.  In order to assess the severity of dermatitis, the checklist of Diaper Rash five-point scale was used. Data were analyzed by software SPSS version 14 using chi-square, ANOVA, T-Test. Results: The age in the Chamomile group was (8. 8 ± 2.3 and in Calendula group was (9.0± 2.4 months. The severity of dermatitis in Chamomile group before treatment was 1.6 ± 0.5, on the third day 0.8 ± 0.4, and on the seventh day zero (p

  9. 原位杂交检测lin-28 mRNA在 Caenorhabditis elegans中的分布%Distribution of lin-28 mRNA in Wild-type and Mutants of C. elegans Via in situ Hybridization

    Institute of Scientific and Technical Information of China (English)

    杨玉荣; 郑卫玲

    2008-01-01

    利用原位杂交检测了异时性基因lin-28 mRNA在野生型和lin-4,lin-14突变体以及lin-4,lin-14双突变体中的分布,发现lin-28 mRNA在胚胎和成虫生殖腺中存在.还发现lin-4突变不改变lin-28 mRNA分布,证明了lin-28表达独立于lin-4抑制通路的存在.

  10. In vitro expansion of Lin{sup +} and Lin{sup −} mononuclear cells from human peripheral blood

    Energy Technology Data Exchange (ETDEWEB)

    Norhaiza, H. Siti; Zarina, Z. A. Intan; Hisham, Z. A. Shahrul [School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Selangor (Malaysia); Rohaya, M. A. W. [Department of Orthodontics, Faculty of Dentistry, Universiti Kebangsaan Malaysia, 50300, Kuala Lumpur (Malaysia)

    2013-11-27

    Haematopoietic stem cells (HSCs) are used in the therapy of blood disorders due to the ability of these cells to reconstitute haematopoietic lineage cells when transplanted into myeloablative recipients. However, substantial number of cells is required in order for the reconstitution to take place. Since HSCs present in low frequency, larger number of donor is required to accommodate the demand of transplantable HSCs. Therefore, in vitro expansion of HSCs will have profound impact on clinical purposes. The aim of this study was to expand lineage negative (Lin{sup −}) stem cells from human peripheral blood. Total peripheral blood mononuclear cells (PBMNCs) were fractionated from human blood by density gradient centrifugation. Subsequently, PBMNCs were subjected to magnetic assisted cell sorter (MACS) which depletes lineage positive (Lin{sup +}) mononuclear cells expressing lineage positive markers such as CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a to obtained Lin{sup −} cell population. The ability of Lin{sup +} and Lin{sup −} to survive in vitro was explored by culturing both cell populations in complete medium consisting of Alpha-Minimal Essential Medium (AMEM) +10% (v/v) Newborn Calf Serum (NBCS)+ 2% (v/v) pen/strep. In another experiment, Lin{sup +} and Lin{sup −} were cultured with complete medium supplemented with 10ng/mL of the following growth factors: stem cell factor (SCF), interleukin (IL)-3, granulocyte-macrophage colony stimulating factor (GM-CSF), 2IU/mL of Erythropoietin (Epo) and 20ng/mL of IL-6. Three samples were monitored in static culture for 22 days. The expansion potential was assessed by the number of total viable cells, counted by trypan blue exclusion assay. It was found that Lin{sup +} mononuclear cells were not able to survive either in normal proliferation medium or proliferation medium supplemented with cytokines. Similarly, Lin{sup −} stem cells were not able to survive in proliferation medium however

  11. The signal pathway of Lin28 and tumor%Lin28信号通路与肿瘤

    Institute of Scientific and Technical Information of China (English)

    荚耘路; 吕可真; 林娜; 胡文献

    2014-01-01

    Lin28 is a highly conserved RNA-binding protein that has an important role in human body development, metabolism, and tumorigenesis. Previous studies have found that the activation of Lin28 can suppress the maturity of let-7 miRNA in stem cells and promote the proliferation of stem cells by upregulating cell-cycle related genes, such as cyclins A and B. Other mechanisms underlying the activation of Lin28 can selectively block the processes of pre-let-7 miRNA in embryonic stem cells and ultimately promote tumori-genesis. In addition, Lin28 affects the occurrence, development, and prognosis of cancer by inhibiting the differentiation and maturation of miRNA and by moderating the expression of some cell-cycle related genes. Lin28 has an important function in the tumor's tolerance of chemotherapy and radiotherapy. We review the physiological function of Lin28 in tumors and its function in tumor treatment.%Lin28是一种结构上高度保守的RNA结合蛋白,其在机体正常生长发育代谢及某些疾病状态,如肿瘤发生发展中有重要作用。近年的研究发现,Lin28在机体中的过表达能够抑制let-7 miRNA的功能,并上调某些细胞周期相关基因如cyclin A、cyclin B等的表达,从而促进干细胞的增殖。在人类恶性肿瘤细胞中,Lin28的激活能选择性地阻断原始let-7 miRNA的加工成熟并最终促进肿瘤的发生;Lin28在肿瘤疾病进程中的其他作用机制包括:通过抑制肿瘤相关microRNA的分化成熟、上调细胞周期相关基因的表达影响肿瘤的发展及预后。另外,Lin28在肿瘤放化疗耐受相关研究中的重要作用日益凸显。为此,本文对Lin28的相关生理功能和其在肿瘤发生发展的相关机制及治疗前景做一综述。

  12. 原位杂交检测历lin-28mRNA在Caenorhabditis elegans中的分布

    Institute of Scientific and Technical Information of China (English)

    杨玉荣; 郑卫玲

    2008-01-01

    利用原位杂交检测了异时性基因lin-28mRNA在野生型和lin-4,lin-14突变体以及lin-4,lin-14双突变体中的分布,发现lin-28mlLNA在胚胎和成虫生殖腺中存在.还发现lin-4突变不改变lin-28mlLNA分布,证明了lin-28表达独立于lin-4抑制通路的存在.

  13. The Policy Issues in Lin an Modei Forest of China

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Based on the analysis on policy such as property rights, tax and administration fee, harvesting and circulation of forest products in Lin 'an Modei Forest, some policy issues in the development of Lin 'an Model Forest towards sustainable forest management were raised: (1) The level of timber tax and fee is quite high and there are many kinds of taxes and fees such as fees for forest silviculture, forestry maintenance, forest protection, city construction, town-service, additional fee for education and o...

  14. lin-8, which antagonizes Caenorhabditis elegans Ras-mediated vulval induction, encodes a novel nuclear protein that interacts with the LIN-35 Rb protein.

    Science.gov (United States)

    Davison, Ewa M; Harrison, Melissa M; Walhout, Albertha J M; Vidal, Marc; Horvitz, H Robert

    2005-11-01

    Ras-mediated vulval development in C. elegans is inhibited by the functionally redundant sets of class A, B, and C synthetic Multivulva (synMuv) genes. Three of the class B synMuv genes encode an Rb/DP/E2F complex that, by analogy with its mammalian and Drosophila counterparts, has been proposed to silence genes required for vulval specification through chromatin modification and remodeling. Two class A synMuv genes, lin-15A and lin-56, encode novel nuclear proteins that appear to function as a complex. We show that a third class A synMuv gene, lin-8, is the defining member of a novel C. elegans gene family. The LIN-8 protein is nuclear and can interact physically with the product of the class B synMuv gene lin-35, the C. elegans homolog of mammalian Rb. LIN-8 likely acts with the synMuv A proteins LIN-15A and LIN-56 in the nucleus, possibly in a protein complex with the synMuv B protein LIN-35 Rb. Other LIN-8 family members may function in similar complexes in different cells or at different stages. The nuclear localization of LIN-15A, LIN-56, and LIN-8, as well as our observation of a direct physical interaction between class A and class B synMuv proteins, supports the hypothesis that the class A synMuv genes control vulval induction through the transcriptional regulation of gene expression.

  15. EFECTO DE EXTRACTOS ACUOSOS DE Helianthus annuus Lin. SOBRE EL CRECIMIENTO DE Solanum lycopersicum Lin.

    Directory of Open Access Journals (Sweden)

    Yaniuska González Perigó

    2015-01-01

    Full Text Available Con el objetivo de determinar el efecto de extractos acuosos de Helianthus annuus Lin. sobre el crecimiento de Solanum lycopersicum Lin, se tomaron muestras de raíz y hojas de plantas recolectadas de un sistema de policultivo ubicado en un agroecosistema montañoso. Los extractos acuosos de girasol se obtuvieron a partir de raíz y hojas de plantas recolectadas durante dos años. Se evaluaron los efectos de estos extractos sobre la germinación, longitud de la radícula y del hipocótilo de semillas de tomate certificadas, para ello se ejecutaron nueve tratamientos con cuatro repeticiones dispuestos en placas Petri. Los extractos de raíz de girasol a los 15, 30 y 45 días y de hojas a los 75 y 90 días de desarrollo inhibieron la germinación de las semillas de tomate y el crecimiento de la radícula y el hipocótilo. Los extractos de raíz de girasol con 75 y 90 días, estimularon el crecimiento de la radícula y del hipocótilo del tomate. Estos resultados demuestran que el girasol produce sustancias químicas que inhiben el crecimiento del tomate y no se recomienda asociar estos dos cultivos de forma simultánea.

  16. MEDICINAL USES AND PHYTOCONSTITUENTS OF PAEONIA OFFICINALIS

    Directory of Open Access Journals (Sweden)

    Ahmad F

    2012-04-01

    Full Text Available Paeonia officinalis (European peony, Common peony has been cultivated in Europe for years. The root has been used medicinally for over 2,000 years mainly in the treatment for epilepsy and to promote menstruation. Root is also antispasmodic, diuretic, sedative and tonic and has been successfully employed in the treatment of convulsions and spasmodic nervous affections such as epilepsy. It has also been used in the treatment of whooping cough whilst suppositories are sometimes made of the root to relieve anal and intestinal spasms, hemorrhoids and varicose veins. Experimentally it has been proved to have antihypertensive, abortifacient action and anti-ulcer activity. The roots of this plant are of great medicinal significance in unani system and homeopathy. The roots contain asparagin, benzoic acid, flavonoids, paeoniflorin, paeonin, paeonol, protoanemonin, tannic acid, triterpenoids, and volatile oil. This review covers botany, traditional uses and the phytoconstituents of the roots of Paeonia officinalis.

  17. Two new acetylenic compounds from Asparagus officinalis.

    Science.gov (United States)

    Li, Xue-Mei; Cai, Jin-Long; Wang, Wen-Xiang; Ai, Hong-Lian; Mao, Zi-Chao

    2016-01-01

    Two new acetylenic compounds, asparoffins A (1) and B (2), together with two known compounds, nyasol (3) and 3″-methoxynyasol (4), were isolated from stems of Asparagus officinalis. The structures of two new compounds were elucidated on the basis of detailed spectroscopic analyses (UV, IR, MS, 1D, and 2D NMR). All compounds were evaluated for their cytotoxicities against three human cancer cell lines.

  18. The Analysis of Lin Yutang’s Humor

    Institute of Scientific and Technical Information of China (English)

    陈晓琴

    2014-01-01

    Lin Yutang is called the master humorist in China. He advocates humor and spiritual literature in his maga-zine Lun Yu (Analects) which attracts essays and readership. His informal but polished style in both Chinese and English makes him one of the most influential writers of his generation, and his compilations and translations of classic Chinese texts into English are bestsellers in the West. Humor is a distinctive feature in his works. In his book, he uses humor as he sees appropriate, and through humor he criticizes social realities, depicts characters and introduces original expressions, etc. His humor can be related to some aspects in his life. In this paper, I will briefly analysis Lin's sense of humor and how Lin' applies humor in his works.

  19. Topical Calendula and Betamethasone Valerate in the prevention of acute radiation dermatitis: a randomized prospective trial

    Directory of Open Access Journals (Sweden)

    Fotouhi M

    2007-07-01

    Full Text Available Background: Acute radiation dermatitis is a very common side effect of radiation therapy for many cancers, including breast cancer. Despite the high prevalence of acute radiation dermatitis as well as wet desquamation, only a few trials studying the prophylaxis of this complication using topical treatment have been conducted. In spite of these studies, some controversy still exists about regarding treatments for acute radiation dermatitis, as does some concern about their long-term complications. For this reason, we conducted a clinical trial for a new treatment with the same effectiveness as corticosteroids, but fewer complications. Methods: This trial included 60 patients with pathologic diagnoses of breast cancer for whom radiotherapy had been planned. Patients were 30-73 years old. Patients with radical mastectomy received 5000 cGy over five weeks, and those with conservative surgery received 6000 cGy over six weeks divided in 200 cGy fractions. Patients were divided randomly into two groups: one group received a moderately-potent glucocorticoid steroid, 0.1% betamethasone ointment (30, and the other received the new treatment, 0.1% calendula ointment (30. All patients applied their respective drugs twice daily within the tangential field from the first day of radiation treatment until one month after treatment was completed. Starting one week after radiation therapy commenced, patients were monitored weekly for symptoms of dermatitis and the degree of severity as well as possible adverse drug effects, in addition to such monitoring on the days of their appointments. Four weeks after termination of therapy, patients were again examined, at which time they completed a questionnaire about dermatologic complications. Results: The mean time to develop dermatitis was 3.7 weeks for the betamethasone group and 3.87 weeks for the calendula group. Maximal dermatitis intensity during treatment in the betamethasone group was: 0, 6.7%; I, 73.3%; II, 16

  20. Eindrapportage Calendula-keten Project : een internationale keten-innovatie-opgave met technologische, economische en sociaal-culturele aspecten

    NARCIS (Netherlands)

    PRI,

    2008-01-01

    Doel van dit project is het duurzaam organiseren van een innovatieve, internationale, agro-industriële keten voor hernieuwbare grondstoffen met Calendula als praktijkvoorbeeld. Daarnaast beoogt het project om in een samenwerking van technologische en gamma-georiënteerde kennisinstellingen te leren h

  1. The Impact of Aloe vera and Calendula on Perineal Healing after Episiotomy in Primiparous Women: A Randomized Clinical Trial

    Directory of Open Access Journals (Sweden)

    Farideh Eghdampour

    2013-11-01

    Full Text Available Introduction: Episiotomy is used for enlarging the perineum. Aloe vera and Calendula have been used for treating different diseases from ancient times, limited researches have been done regarding the healing of these plants. Since the effect of their ointment on episiotomy healing has not been studied, this study is being done for determining the impact of Aloe vera and Calendula on episiotomy healing in primiparous women. Methods: This clinical trial involves 111 qualified primiparous women admitted in Lolagar hospital. They were randomly categorized into three groups of control (n=1 and experimental (n=2 groups. The women in experimental group used Aloe vera and Calendula Ointment every 8 hours and the control group used hospital routine on episiotomy for 5 days. The data were collected by demographic questionnaire and redness, edema, ecchymosis, discharge and approximation scale (REEDA which investigated the episiotomy healing before and five days after intervention in two groups. ANOVA, Tukey test, Kruskal-wallis, Chi-square were used for data analysis. Results: The three groups do not have statistically significant different regarding demographic and other intervening variables. Comparing the mean of REEDA in five days after delivery showed statistically significant difference between control and experimental groups.Conclusion: According to the results, using Aloe vera and Calendula ointment considerably increases the speed of episiotomy wound healing so it can be used for quickening the episiotomy healing.

  2. Study of calendula and Gaillardia growth in two composts prepared from agroindustrial wastes.

    Science.gov (United States)

    Roudsari, O Nouri; Akbari, B

    2007-05-01

    Two composts prepared from agroindustrial wastes were assayed as substrates: C1 from brewing waste (yeast and malt) plus lemon tree pruning and C2 from the solid fraction of olive mill wastewater plus olive leaves. Sixteen substrates were prepared by combining each compost with Sphagnum peat or a Commercial Substrate (CS) in different proportions. The nutrients (N and K) provided by the composts, which acted as slow-release fertilizers, influenced especially the development of calendula, although the physical and physicochemical properties such as total pore space and Electrical Conductivity (EC) were also relevant. On the other hand, in the salt-sensitive Gaillardia hybrid, EC and chloride concentration were the main factors influencing growth. The best results were found in substrates prepared by mixing C1 at up to 75% with peat, or at up to 50% with CS, or by mixing C2 at up to 50% with peat or CS, for calendula. For salt-sensitive species such as Gaillardia, adequate substrates for plant development were found for C1 at up to 50% with peat or CS, but the use of C2 should be limited to 25% in mixtures with peat or CS. Therefore, composts of agroindustrial origin such as these can be used as an alternative to peat and CSs for growing ornamental plants, provided the mixture contains at least 25% peat or CS.

  3. Morphological and Biological Study of Sanguisorba Officinalis Germination

    Directory of Open Access Journals (Sweden)

    Alexandra Sh. Dodonova

    2013-01-01

    Full Text Available This work deals with the study of influence of terms and storage conditions on Sanguisorba officinalis seed material quality, put into various containers (paper, plastic, fabric, glass, in the different temperature conditions, light and dark grown. The morphology, biology of Sanguisorba officinalis seeds was described and experiments on cryopreservation were made. Basing on the study, we recommend to store Sanguisorba officinalis seed material within 3 months at a temperature of +4˚С in glass container, use plastic container for cryopreservation and to defreeze seeds by double boiling.

  4. The pluripotency factor LIN28 marks undifferentiated spermatogonia in mouse

    Directory of Open Access Journals (Sweden)

    Kaestner Klaus H

    2009-06-01

    Full Text Available Abstract Background Life-long production of spermatozoa depends on spermatogonial stem cells. Spermatogonial stem cells exist among the most primitive population of germ cells – undifferentiated spermatogonia. Transplantation experiments have demonstrated the functional heterogeneity of undifferentiated spermatogonia. Although the undifferentiated spermatogonia can be topographically divided into As (single, Apr (paired, and Aal (aligned spermatogonia, subdivision of this primitive cell population using cytological markers would greatly facilitate characterization of their functions. Results In the present study, we show that LIN28, a pluripotency factor, is specifically expressed in undifferentiated spermatogonia (As, Apr, and Aal in mouse. Ngn3 also specifically labels undifferentiated spermatogonia. We used Ngn3-GFP knockin mice, in which GFP expression is under the control of all Ngn3 transcription regulatory elements. Remarkably, Ngn3-GFP is only expressed in ~40% of LIN28-positive As (single cells. The percentage of Ngn3-GFP-positive clusters increases dramatically with the chain length of interconnected spermatogonia. Conclusion Our study demonstrates that LIN28 specifically marks undifferentiated spermatogonia in mice. These data, together with previous studies, suggest that the LIN28-expressing undifferentiated spermatogonia exist as two subpopulations: Ngn3-GFP-negative (high stem cell potential and Ngn3-GFP-positive (high differentiation commitment. Furthermore, Ngn3-GFP-negative cells are found in chains of Ngn3-GFP-positive spermatogonia, suggesting that cells in the Aal spermatogonia could revert to a more primitive state.

  5. File list: Oth.Lar.05.lin-35.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Lar.05.lin-35.AllCell ce10 TFs and others lin-35 Larvae SRX331086,SRX233462,SRX...059240,SRX233461,SRX331088,SRX059241 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Oth.Lar.05.lin-35.AllCell.bed ...

  6. The lin-15 locus encodes two negative regulators of Caenorhabditis elegans vulval development.

    Science.gov (United States)

    Huang, L S; Tzou, P; Sternberg, P W

    1994-01-01

    During Caenorhabditis elegans vulval development, an inductive signal from the anchor cell stimulates three of the six vulval precursor cells (VPCs) to adopt vulval rather than nonvulval epidermal fates. Genes necessary for this induction include the lin-3 growth factor, the let-23 receptor tyrosine kinase, and let-60 ras. lin-15 is a negative regulator of this inductive pathway. In lin-15 mutant animals, all six VPCs adopt vulval fates, even in the absence of inductive signal. Previous genetic studies suggested that lin-15 is a complex locus with two independently mutable activities, A and B. We have cloned the lin-15 locus by germline transformation and find that it encodes two nonoverlapping transcripts that are transcribed in the same direction. The downstream transcript encodes the lin-15A function; the upstream transcript encodes the lin-15B function. The predicted lin-15A and lin-15B proteins are novel and hydrophilic. We have identified a molecular null allele of lin-15 and have used it to analyze the role of lin-15 in the signaling pathway. We find that lin-15 acts upstream of let-23 and in parallel to the inductive signal. Images PMID:8054684

  7. Ethnobotanical and phytopharmacological review of Scindapsus officinalis (“Gajapippali”

    Directory of Open Access Journals (Sweden)

    Kuljeet Kaur

    2017-01-01

    Full Text Available Scindapsus officinalis (S. officinalis holds a reputed position in Ayurvedic system of medicine. It has been ethanobotanically used to treat diarrhea (“atisara”, worm infestation (“krmiroga”, and as antipyretic. Literature survey on S. officinalis was carried out via electronic search in PubMed, SciFinder, Scirus, Google Scholar, Agricola and Web of Science and a library search. Results revealed that a very specific botanical description of the plant is still not available. The plant is mistaken within the hybrids and other plants of genus Scindapsus and family Araceae. Since ethnobotanically the plant is of much importance, chemistry of the plant yet needs to be fully explored. Thus the need of the hour is to comprehend the fragmented information available on the botany, traditional uses, phytochemistry and pharmacology of S. officinalis which could help in the correct identification of the sample and avoid adulteration due to mistaken identity.

  8. In vitro and ex vivo antiangiogenic activity of Salvia officinalis.

    Science.gov (United States)

    Keshavarz, Maryam; Mostafaie, Ali; Mansouri, Kamran; Bidmeshkipour, Ali; Motlagh, Hamid Reza Mohammadi; Parvaneh, Shahram

    2010-10-01

    Angiogenesis is a key process in the promotion of cancer and its metastasis. Herein, the antiangiogenic activity of Salvia officinalis extract and its fractions was investigated. S. officinalis aerial parts were extracted with ethanol and its successive hexane, ethyl acetate, n-butanol and aqueous fractions were evaluated for their antiangiogenic activities using human umbilical vein endothelial cells (HUVEC) capillary tube formation and rat aorta models in a three-dimensional collagen matrix. Furthermore, antimigrative effects of the fractions were assessed using a wound healing model. The ethanol extract of S. officinalis (ESO) potently inhibited capillary tube formation in HUVEC and rat aorta models of angiogenesis, and its hexane fraction (HSO) exerted the highest inhibitory effect. In addition, the ethanol extract of S. officinalis and its hexane fraction showed a dose-dependent inhibitory activity on the migration of the endothelial cells in the wound healing model. Furthermore, ESO inhibited endothelial cell proliferation at 50-200 μg/mL in a dose-dependent manner. These findings indicated some new pharmacological activities of S. officinalis such as antiangiogenic in vitro and ex vivo, and antimigrative activity in vitro. Therefore, S. officinalis could be a candidate as a useful herb with therapeutic or preventive activity against angiogenesis related disorders.

  9. The C. elegans LIM homeobox gene lin-11 specifies multiple cell fates during vulval development.

    Science.gov (United States)

    Gupta, Bhagwati P; Wang, Minqin; Sternberg, Paul W

    2003-06-01

    LIM homeobox family members regulate a variety of cell fate choices during animal development. In C. elegans, mutations in the LIM homeobox gene lin-11 have previously been shown to alter the cell division pattern of a subset of the 2 degrees lineage vulval cells. We demonstrate multiple functions of lin-11 during vulval development. We examined the fate of vulval cells in lin-11 mutant animals using five cellular markers and found that lin-11 is necessary for the patterning of both 1 degrees and 2 degrees lineage cells. In the absence of lin-11 function, vulval cells fail to acquire correct identity and inappropriately fuse with each other. The expression pattern of lin-11 reveals dynamic changes during development. Using a temporally controlled overexpression system, we show that lin-11 is initially required in vulval cells for establishing the correct invagination pattern. This process involves asymmetric expression of lin-11 in the 2 degrees lineage cells. Using a conditional RNAi approach, we show that lin-11 regulates vulval morphogenesis. Finally, we show that LDB-1, a NLI/Ldb1/CLIM2 family member, interacts physically with LIN-11, and is necessary for vulval morphogenesis. Together, these findings demonstrate that temporal regulation of lin-11 is crucial for the wild-type vulval patterning.

  10. The Effects of Medicinal Plants of Melissa officinalis and Salvia officinalis on Primary Dysmenorrhea

    Directory of Open Access Journals (Sweden)

    R. Kalvandi

    2014-07-01

    Full Text Available Introduction & Objective: Primary dysmenorrhea is one of the common problems in women, especially in young women. Although dysmenorrhea is not life threatening , it can have ad-verse effect on quality of life and cause disability or ineffectiveness. With regard to the young society suffering from it, various treatments are offered for dysmenorrhea one of which is medicinal plants. This study investigated the effect of Melissa officinalis and Salvia officinalis on primary dysmenorrhea Materials & Methods: This study was a clinical trial study performed on 50 girls with dys-menorrheal taking Salvia officinalis as infusion for five days before menstruation and five days during the menstruation. The consumption of the plants was repeated in the next two periods in the same way. Severity of pain, blooding duration and dysmenorrhea duration were evaluated with VAS (Visual Analog Scale Questionnaire in their next two cycles. Data were analyzed by chi-square, t-test and WILCOXON statistical test. Results: The results of this study showed that after using the plants, pain severity and pain duration significantly reduced P<0.01so that pain severity decreased from 6.30 to 3.94 and 3.24, (on a scale of 1 to 10 after taking the plants in the first and second periods ,respectively. The maximum pain duration before the treatment was 1 to 6 h (28.6% and this number reduced to less than 1 h after using these medicinal plants (38.0%. Mean of blooding duration was 6.36 days before the treatment. After the first and second duration, this value reached 6.48 and 6.34 days, respectively. It was determined that taking oral seda-tives reduced from 56% to 26% and 22% after the first and second periods, respectively. Fi-nally, after the second period, 42% of the girls reported high rates of satisfaction because of using the medicinal plants. Conclusion: Generally, it was showed that Melissa officinalis and Salvia officinalis signifi-cantly reduced pain severity and pain

  11. 金盏菊花黄色素的性质研究%Property of yellow pigment from Calendula officinalis L

    Institute of Scientific and Technical Information of China (English)

    胡晓丹; 谢笔钧; 王建中

    2001-01-01

    该文采用溶剂提取法和紫外可见分光光度法,研究了金盏菊花黄色素的提取方法、稳定性和抗氧化性以及色素提取物的各项指标.结果表明:金盏菊花黄色素主要是类胡萝卜素,用等体积的石油醚和丙酮作浸提剂有较高的提取率,所得提取物呈红色油状液体,具有油溶性,各项指标均符合国家标准;该色素对热、酸、碱、氧化剂、还原剂、室内散射光、紫外光、络合剂及大部分金属离子稳定,但对日光及少数金属离子(如Fe3+,Al3+,Cu2+)不稳定;该色素有显著的抗油脂氧化效果.

  12. Protective effects of ψ taraxasterol 3-O-myristate and arnidiol 3-O-myristate isolated from Calendula officinalis on epithelial intestinal barrier.

    Science.gov (United States)

    Dall'Acqua, Stefano; Catanzaro, Daniela; Cocetta, Veronica; Igl, Nadine; Ragazzi, Eugenio; Giron, Maria Cecilia; Cecconello, Laura; Montopoli, Monica

    2016-03-01

    The triterpene esters ᴪ taraxasterol-3-O-myristate (1) and arnidiol-3-O-myristate (2) were tested for their ability to protect epithelial intestinal barrier in an in vitro model. Their effects on ROS production and on trans-epithelial resistance were investigated on CaCo-2 cell monolayers both in basal and stress-induced conditions. Both compounds were able to modulate the stress damage induced by H2O2 and INFγ+TNFα, showing a potential use as model compounds for the study of new therapeutic agents for intestinal inflammations.

  13. Study on the morphology and histology of Calendula officinalis%金盏菊的形态组织学研究

    Institute of Scientific and Technical Information of China (English)

    郑佳; 卢先明; 高必兴; 辛天怡

    2014-01-01

    目的 研究金盏菊的形态组织学特征.方法 采用生药学常规方法对金盏菊进行性状鉴别、显微鉴别.结果 确定了金盏菊的药材性状、组织构造及粉末的主要鉴别特征.结论 组织学特征可作为金盏菊的鉴别依据.

  14. Studies on Fertilization Effect and Fertilizer Formula Screen for Calendula officinalis L.%盆栽金盏菊氮磷钾肥料配方的研究

    Institute of Scientific and Technical Information of China (English)

    蒋志平; 鲁剑巍; 李文西; 邹娟

    2008-01-01

    采用"3414"不完全正交回归设计,利用盆栽试验研究金盏菊氮磷钾肥施用效果,并筛选适宜的肥料配方.结果表明,氮肥和磷肥对金盏菊的营养生长和生殖生长均有较大影响,而钾肥的效果主要表现在生长后期,氮、磷间有明显的正交互作用.以金盏菊总花数、功能叶片数、株高、植株干样质量为指标进行统计分析,得出盆栽(每盆装基质2 kg)金盏菊氮(N)、磷(P2O5)、钾(K2O)的最佳施用量为0.4、0.2和0.3 g·kg-1.

  15. The flavonoid glycosides from Rwandan Calendula officinalis L.%卢旺达产金盏菊花的黄酮苷类成分

    Institute of Scientific and Technical Information of China (English)

    陈玥; 石萍萍; 李晓霞; 刘丽丽; 王涛; 张祎

    2014-01-01

    目的 对卢旺达产金盏菊花的化学成分进行研究,为进一步开发利用该植物资源提供依据.方法 采用大孔吸附树脂、正相硅胶、Sephadex LH-20、反相ODS等柱色谱以及制备型高效液相色谱法等手段进行分离纯化,并通过理化性质与核磁共振等波谱学数据相结合的方法鉴定化合物的结构.结果 从金盏菊花体积分数为70%的乙醇溶液提取物中分离鉴定了6个单体成分,分别为异鼠李素-3-O-芸香糖苷(isorhamnetin-3-O-rutinoside,1)、异鼠李素-3-O-新橙皮糖苷(isorhamnetin-3-O-neohespeidoside,2)、香蒲新苷(typhaneoside,3)、槲皮素-3-O-β-D-新橙皮糖苷(quercetin-3-O-β-D-neohespeidoside,4)、芦丁(rutin,5)、槲皮素-3-O-(2”,6”-二-O-α-L-鼠李糖基)-β-D-葡萄糖苷(quercetin-3-O-(2",6"-di-O-α-L-rhamnopyranosyl)-β-D-glucopyranoside,6).结论 首次对东非产金盏菊干燥花的化学成分进行研究,分离得到了6个黄酮类化合物.

  16. A Dynamic Attitude Measurement System Based on LINS

    Directory of Open Access Journals (Sweden)

    Hanzhou Li

    2014-08-01

    Full Text Available A dynamic attitude measurement system (DAMS is developed based on a laser inertial navigation system (LINS. Three factors of the dynamic attitude measurement error using LINS are analyzed: dynamic error, time synchronization and phase lag. An optimal coning errors compensation algorithm is used to reduce coning errors, and two-axis wobbling verification experiments are presented in the paper. The tests indicate that the attitude accuracy is improved 2-fold by the algorithm. In order to decrease coning errors further, the attitude updating frequency is improved from 200 Hz to 2000 Hz. At the same time, a novel finite impulse response (FIR filter with three notches is designed to filter the dither frequency of the ring laser gyro (RLG. The comparison tests suggest that the new filter is five times more effective than the old one. The paper indicates that phase-frequency characteristics of FIR filter and first-order holder of navigation computer constitute the main sources of phase lag in LINS. A formula to calculate the LINS attitude phase lag is introduced in the paper. The expressions of dynamic attitude errors induced by phase lag are derived. The paper proposes a novel synchronization mechanism that is able to simultaneously solve the problems of dynamic test synchronization and phase compensation. A single-axis turntable and a laser interferometer are applied to verify the synchronization mechanism. The experiments results show that the theoretically calculated values of phase lag and attitude error induced by phase lag can both match perfectly with testing data. The block diagram of DAMS and physical photos are presented in the paper. The final experiments demonstrate that the real-time attitude measurement accuracy of DAMS can reach up to 20″ (1σ and the synchronization error is less than 0.2 ms on the condition of three axes wobbling for 10 min.

  17. Translator and Identity—Taking Lin Yutang as an Example

    Institute of Scientific and Technical Information of China (English)

    任春燕

    2016-01-01

    with the prosperous development of translation, the identity of translator has attracted more and more attention. The relationship of translator and identity is worth studying. This article takes translator Lin Yutang as an example to see how identities influence a translator. The conclusion is that the choice of translation martial, translation strategy, translation attitude and other aspects in translation are influenced by Li Yutang's identities such as his religious identity, class identity, national identity and personal identity.

  18. Monoterpene synthases from common sage (Salvia officinalis)

    Energy Technology Data Exchange (ETDEWEB)

    Croteau, Rodney Bruce (Pullman, WA); Wise, Mitchell Lynn (Pullman, WA); Katahira, Eva Joy (Pullman, WA); Savage, Thomas Jonathan (Christchurch 5, NZ)

    1999-01-01

    cDNAs encoding (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase from common sage (Salvia officinalis) have been isolated and sequenced, and the corresponding amino acid sequences has been determined. Accordingly, isolated DNA sequences (SEQ ID No:1; SEQ ID No:3 and SEQ ID No:5) are provided which code for the expression of (+)-bornyl diphosphate synthase (SEQ ID No:2), 1,8-cineole synthase (SEQ ID No:4) and (+)-sabinene synthase SEQ ID No:6), respectively, from sage (Salvia officinalis). In other aspects, replicable recombinant cloning vehicles are provided which code for (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase, or for a base sequence sufficiently complementary to at least a portion of (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant monoterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase may be used to obtain expression or enhanced expression of (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase in plants in order to enhance the production of monoterpenoids, or may be otherwise employed for the regulation or expression of (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase, or the production of their products.

  19. Inhibitory effects of Sanguisorba officinalis L.on MRSA biofilms formation%地榆对MRSA生物被膜形成的抑制作用

    Institute of Scientific and Technical Information of China (English)

    王馨; 陈菲; 王龙; 谢鲲鹏; 谢明杰

    2016-01-01

    Objective To investigate inhibitory effects of Sanguisorba officinalis L.on biofilms of MRSA41577.Methods Congo red agar method and crystal violet semi-quantitative method were used to detect the biofilms-forming ability of tested strains; TTC assay was used to detect inhibitory effects of Sanguisorba officinalis L.on biofilms formation and mature biofilms of MRSA41577,as well as effects of Sanguisorba officinalis L.in combination with vancomycin on mature biofilms of MRSA41577.Results Sanguisorba officinalis L.showed significant inhibitory both on biofilms formation and mature biofilms, minimum inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) of biofilms formation were 1 mg/mL and 8 mg/mL,MIC of mature biofilms was 4 mg/mL.The sensitivity of mature biofilms to vancomycin was greatly increased when Sanguisorba officinalis L.was combined with vancomycin with subinhibitory concentrations.Sanguisorba officinalis L.at 1/4 MIC can inhibit mature biofilms when combined with vancomycin at 4 μg/mL, while vancomycin didn't show inhibitory effects on mature biofilms when concentrations were below 64 μg/mL. Conclusion Sanguisorba officinalis L.has significant inhibitory on biofilms formation, the mechanism may be related to Sanguisorba officinalis L.destroyed biofilms and make vancomycin penetrate into the biofilms to finish the bactericidal activity.%目的:研究地榆对MRSA41577菌株生物被膜( bacterial biofilm,BF)的抑制作用。方法采用刚果红法和结晶紫半定量法检测供试菌株BF的形成能力;采用氯化三苯基四氮唑( TTC)法测定地榆对MRSA41577BF形成和成熟BF的抑制作用,以及地榆与万古霉素联用对MRSA41577成熟BF的抑制作用。结果地榆对MRSA41577BF形成和成熟BF有显著的抑制作用,其抑制MRSA41577BF形成的最低抑菌浓度(minimum inhibitory concentration, MIC)和最低杀菌浓度(minimal bactericidal concentration,MBC)分别为1 mg

  20. Detection of lin-4 mRNA in Caenorhabditis elegans Wild Type and Mutants via in Situ Hybridization%原位杂交检测lin-4 mRNA在Caenorhabditis elegans中的表达

    Institute of Scientific and Technical Information of China (English)

    高雅君; 杨玉荣

    2007-01-01

    lin-4是控制Caenorhabditis elegans(C.elegans)幼虫发育的异时性基因,也是一种小RNA分子(microRNA).通过整体原位杂交检测小RNA lin-4在野生型和lin-14、lin-28突变体中的区域性表达,探讨lin-4在C.elegans发育时空控制中的作用.结果表明:lin-4 mRNA在胚胎发育的早期和中期表达,胚胎后期至L1期末没有表达,之后又持续表达,成虫中也可以检测到lin-4 mRNA的存在.在lin-14和lin-28突变体中,lin-4的表达基本与野生型一致,不受lin-14、lin-28基因突变的影响,说明lin-14和lin-28是lin-4的下游基因.

  1. Lin-28 promotes symmetric stem cell division and drives adaptive growth in the adult Drosophila intestine.

    Science.gov (United States)

    Chen, Ching-Huan; Luhur, Arthur; Sokol, Nicholas

    2015-10-15

    Stem cells switch between asymmetric and symmetric division to expand in number as tissues grow during development and in response to environmental changes. The stem cell intrinsic proteins controlling this switch are largely unknown, but one candidate is the Lin-28 pluripotency factor. A conserved RNA-binding protein that is downregulated in most animals as they develop from embryos to adults, Lin-28 persists in populations of adult stem cells. Its function in these cells has not been previously characterized. Here, we report that Lin-28 is highly enriched in adult intestinal stem cells in the Drosophila intestine. lin-28 null mutants are homozygous viable but display defects in this population of cells, which fail to undergo a characteristic food-triggered expansion in number and have reduced rates of symmetric division as well as reduced insulin signaling. Immunoprecipitation of Lin-28-bound mRNAs identified Insulin-like Receptor (InR), forced expression of which completely rescues lin-28-associated defects in intestinal stem cell number and division pattern. Furthermore, this stem cell activity of lin-28 is independent of one well-known lin-28 target, the microRNA let-7, which has limited expression in the intestinal epithelium. These results identify Lin-28 as a stem cell intrinsic factor that boosts insulin signaling in intestinal progenitor cells and promotes their symmetric division in response to nutrients, defining a mechanism through which Lin-28 controls the adult stem cell division patterns that underlie tissue homeostasis and regeneration.

  2. Rosmarinus officinalis essential oil: antiproliferative, antioxidant and antibacterial activities

    Directory of Open Access Journals (Sweden)

    Abdullah Ijaz Hussain

    2010-12-01

    Full Text Available The aim of this work was to investigate and compare the antiproliferative, antioxidant and antibacterial activities of Rosmarinus officinalis essential oil, native to Pakistan. The essential oil content from the leaves of R. officinalis was 0.93 g 100g-1. The GC and GC-MS analysis revealed that the major components determined in R. officinalis essential oil were 1,8-cineol (38.5%, camphor (17.1%, α-pinene (12.3%, limonene (6.23%, camphene (6.00% and linalool (5.70%. The antiproliferative activity was tested against two cancer (MCF-7 and LNCaP and one fibroblast cell line (NIH-3T3 using the MTT assay, while, the antioxidant activity was evaluated by the reduction of 2, 2-diphenyl-1-picryl hydrazyl (DPPH and measuring percent inhibition of peroxidation in linoleic acid system. The disc diffusion and modified resazurin microtitre-plate assays were used to evaluate the inhibition zones (IZ and minimum inhibitory concentration (MIC of R. officinalis essential oil, respectively. It is concluded from the results that Rosmarinus officinalis essential oil exhibited antiproliferative, antioxidant and antibacterial activities.

  3. lin-28 controls the succession of cell fate choices via two distinct activities.

    Directory of Open Access Journals (Sweden)

    Bhaskar Vadla

    Full Text Available lin-28 is a conserved regulator of cell fate succession in animals. In Caenorhabditis elegans, it is a component of the heterochronic gene pathway that governs larval developmental timing, while its vertebrate homologs promote pluripotency and control differentiation in diverse tissues. The RNA binding protein encoded by lin-28 can directly inhibit let-7 microRNA processing by a novel mechanism that is conserved from worms to humans. We found that C. elegans LIN-28 protein can interact with four distinct let-7 family pre-microRNAs, but in vivo inhibits the premature accumulation of only let-7. Surprisingly, however, lin-28 does not require let-7 or its relatives for its characteristic promotion of second larval stage cell fates. In other words, we find that the premature accumulation of mature let-7 does not account for lin-28's precocious phenotype. To explain let-7's role in lin-28 activity, we provide evidence that lin-28 acts in two steps: first, the let-7-independent positive regulation of hbl-1 through its 3'UTR to control L2 stage-specific cell fates; and second, a let-7-dependent step that controls subsequent fates via repression of lin-41. Our evidence also indicates that let-7 functions one stage earlier in C. elegans development than previously thought. Importantly, lin-28's two-step mechanism resembles that of the heterochronic gene lin-14, and the overlap of their activities suggests a clockwork mechanism for developmental timing. Furthermore, this model explains the previous observation that mammalian Lin28 has two genetically separable activities. Thus, lin-28's two-step mechanism may be an essential feature of its evolutionarily conserved role in cell fate succession.

  4. Therapeutic Inhibitors of LIN28/let-7 Pathway in Ovarian Cancer

    Science.gov (United States)

    2015-09-01

    AWARD NUMBER: W81XWH-14-1-0138 TITLE: Therapeutic Inhibitors of LIN28/let-7 Pathway in Ovarian Cancer PRINCIPAL INVESTIGATOR: John P. Hagan...2015 4. TITLE AND SUBTITLE Therapeutic Inhibitors of LIN28/let-7 Pathway in Ovarian Cancer 5a. CONTRACT NUMBER 5b. GRANT NUMBER W81XWH-14-1...prognosis in multiple cancer types, including ovarian. Silent in almost all adult cells, the Lin28/let-7 pathway is implicated directly in cancer stem

  5. An in-depth review on the medicinal flora Rosmarinus officinalis (Lamiaceae).

    Science.gov (United States)

    Begum, Asia; Sandhya, Subarda; Shaffath Ali, Syed; Vinod, Kombath Ravindran; Reddy, Swapna; Banji, David

    2013-01-01

    Rosmarinus officinalis (Rosemary) is a common household plant which belongs to the family Lamiaceae and is grown in many parts of the world. It is a woody, perennial herb with fragrant, evergreen, needle-like leaves and white, pink, purple or blue flowers. The two most commonly grown hardy Rosemaries are Rosmarinus officinalis 'Arp' and R. officinalis 'Madelene Hill' (syn. 'Hill Hardy'). The other cultivars of the plant are R. officinalis 'Albus', R. officinalis 'Bendenen Blue', R. officinalis 'Goodwin Creek', R. officinalis 'Herb Cottage', R. officinalis 'Logee's Light Blue', R. officinalis 'Miss Jessup's Upright', R. officinalis 'Russian River', R. officinalis 'Salem'. The chemical constituents include bitter principle, resin, tannic acid, volatile oils and fl avonoids. The volatile oil consists of borneol, bornyl acetate, camphene, cineol, pinene and camphor. It is used for problems involved in central nervous system, cardio vascular system, genito urinary conditions, liver treatments, reproductive system and respiratory system. The volatile oil of the plant is used in oils and lotions for the treatment of various ailments like arthritis, gout, muscular pain, neuralgia, wound and rubbed into hair for stimulating the hair bulbs to renewed activity, to prevent premature baldness.

  6. Chemical constituents from Cornus officinalis and their biological activity 1

    Directory of Open Access Journals (Sweden)

    Zhan-Ying Ma

    2012-01-01

    Full Text Available Objective: To study the chemical constituents from Cornus officinalis Sieb., Et Zucc, and their peroxisome proliferator-activated receptors (PPARs agonist activity. Materials and Methods: The leaves of C. officinalis were extracted three times with 90% EtOH at room temperature. The ethanol extracts were combined and concentrated under reduced pressure to yield residue, which was isolated and purified by silica gel and reverse-phase C 18 column chromatography. The structures were elucidated on the basis of spectroscopic evidence and their physiochemical characteristics. Cell-based luciferase reporter gene assays were used to evaluate PPARα/γ agonistic activities. Results: Five compounds were isolated and elucidated as 10-hydroxyhastatoside (1, β-dihydrocornin (2, isoquercitrin (3, loganin (4 and oleanolic acid (5. Conclusion: Compounds 1 and 2 were obtained from C. officinalis for the first time. Compound 3 exhibited moderate agonistic activities for PPARα, with EC 50 values of 29.5 μM.

  7. Selective blockade of microRNA processing by Lin-28

    OpenAIRE

    Viswanathan, Srinivas R.; Daley, George Q.; Gregory, Richard I.

    2008-01-01

    MicroRNAs (miRNAs) play critical roles in development, and dysregulation of miRNA expression has been observed in human malignancies. Recent evidence suggests that the processing of several primary miRNA transcripts (pri-miRNAs) is blocked post-transcriptionally in embryonic stem (ES) cells, embryonal carcinoma (EC) cells, and primary tumors. Here we show that Lin-28, a developmentally regulated RNA-binding protein, selectively blocks the processing of pri-let-7 miRNAs in embryonic cells. Usi...

  8. Brecht e Ivan Lins: Um diálogo intertextual

    Directory of Open Access Journals (Sweden)

    Eloá Heise

    2000-11-01

    Full Text Available Das Thema dieses Aufsatzes betrifft die Verbindungen und Wechselwirkungen zwischen Dichtern und ihren Werken über sprachliche und politische Grenzen hinweg. Am Beispiel von Brechts Gedicht An die Nachgeborenen (1938 und des Songs Aos nossos filhos (An unsere Kinder - Ende der 70er Jahre von Ivan Lins und Vitor Martins wird gezeigt, daß die Interpretation nicht auf eine immanente Perspektive beschränkt werden kann. Um eine angemessene Analyse geben zu können, ist es notwendig, den historischen und sozio-politischen Kontext in Betracht zu ziehen.

  9. Targeting ornithine decarboxylase reverses the LIN28/Let-7 axis and inhibits glycolytic metabolism in neuroblastoma.

    Science.gov (United States)

    Lozier, Ann M; Rich, Maria E; Grawe, Anissa Pedersen; Peck, Anderson S; Zhao, Ping; Chang, Anthony Ting-Tung; Bond, Jeffrey P; Sholler, Giselle Saulnier

    2015-01-01

    LIN28 has emerged as an oncogenic driver in a number of cancers, including neuroblastoma (NB). Overexpression of LIN28 correlates with poor outcome in NB, therefore drugs that impact the LIN28/Let-7 pathway could be beneficial in treating NB patients. The LIN28/Let-7 pathway affects many cellular processes including the regulation of cancer stem cells and glycolytic metabolism. Polyamines, regulated by ornithine decarboxylase (ODC) modulate eIF-5A which is a direct regulator of the LIN28/Let-7 axis. We propose that therapy inhibiting ODC will restore balance to the LIN28/Let-7 axis, suppress glycolytic metabolism, and decrease MYCN protein expression in NB. Difluoromethylornithine (DFMO) is an inhibitor of ODC in clinical trials for children with NB. In vitro experiments using NB cell lines, BE(2)-C, SMS-KCNR, and CHLA90 show that DFMO treatment reduced LIN28B and MYCN protein levels and increased Let-7 miRNA and decreased neurosphere formation. Glycolytic metabolic activity decreased with DFMO treatment in vivo. Additionally, sensitivity to DFMO treatment correlated with LIN28B overexpression (BE(2)-C>SMS-KCNR>CHLA90). This is the first study to demonstrate that DFMO treatment restores balance to the LIN28/Let-7 axis and inhibits glycolytic metabolism and neurosphere formation in NB and that PET scans may be a meaningful imaging tool to evaluate the therapeutic effects of DFMO treatment.

  10. LIN28 binds messenger RNAs at GGAGA motifs and regulates splicing factor abundance.

    Science.gov (United States)

    Wilbert, Melissa L; Huelga, Stephanie C; Kapeli, Katannya; Stark, Thomas J; Liang, Tiffany Y; Chen, Stella X; Yan, Bernice Y; Nathanson, Jason L; Hutt, Kasey R; Lovci, Michael T; Kazan, Hilal; Vu, Anthony Q; Massirer, Katlin B; Morris, Quaid; Hoon, Shawn; Yeo, Gene W

    2012-10-26

    LIN28 is a conserved RNA-binding protein implicated in pluripotency, reprogramming, and oncogenesis. It was previously shown to act primarily by blocking let-7 microRNA (miRNA) biogenesis, but here we elucidate distinct roles of LIN28 regulation via its direct messenger RNA (mRNA) targets. Through crosslinking and immunoprecipitation coupled with high-throughput sequencing (CLIP-seq) in human embryonic stem cells and somatic cells expressing exogenous LIN28, we have defined discrete LIN28-binding sites in a quarter of human transcripts. These sites revealed that LIN28 binds to GGAGA sequences enriched within loop structures in mRNAs, reminiscent of its interaction with let-7 miRNA precursors. Among LIN28 mRNA targets, we found evidence for LIN28 autoregulation and also direct but differing effects on the protein abundance of splicing regulators in somatic and pluripotent stem cells. Splicing-sensitive microarrays demonstrated that exogenous LIN28 expression causes widespread downstream alternative splicing changes. These findings identify important regulatory functions of LIN28 via direct mRNA interactions.

  11. Salvia officinalis L. coverage on plants development

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    C.T.A. CRUZ-SILVA

    Full Text Available ABSTRACT Medicinal plants with essential oils in their composition havetypicallybeen shown to be promising in plant control. Sage (Salvia officinalis L. is cited for its allelopathic effects. This study evaluated the allelopathic potential of dried sage leaves in vegetation, soil and the development of Lycopersicon esculentum Mill. (tomato, Panicum maximum Jacq. (guinea grass and Salvia hispanica L. (chia plants. Three seedlings were transplanted seven days after germination in 1 kg plastic containers with soil, in a greenhouse. The grinded dry mass of sage was placed at rates of 3.75; 7.5 15 t ha-1, and a control (no mass. After 30 days, the chlorophyll index of tomato and guinea grass plants were inhibited with 7.5 and 15 t ha-1 sage cover crops. Tomato shoot length was inhibited in all tested rates, and guinea grass plants showed some reduction in growth when using the highest rate of sage mass (15 t ha-1. The dry mass of tomato and guinea grass plants was reduced when using the15 t ha-1, and 7.5 and 15 t ha-1 of sage cover crops, respectively. It can be concluded that there was some effect of sage coverage on the soil in tomato and guinea grass, but no effect was observed on chia plants.

  12. Constituintes das sementes de Copaifera officinalis L. Constituents from Copaifera officinalis L. seeds

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    Valdir F. Veiga Junior

    2007-01-01

    Full Text Available O gênero Copaifera L. é um dos mais importantes economicamente na Região Amazônica devido, principalmente, à produção dos óleos de copaíba, oléo-resinas com diversas propriedades farmacológicas confirmadas. Apesar disso, os estudos fitoquímicos com as sementes das árvores do gênero Copaifera L.são raros. Copaifera officinalis foi a primeira espécie do gênero Copaifera a ser descrita. Este trabalho descreve a composição dos extratos obtidos em hexano e em acetato de etila das sementes de C. officinalis. No extrato obtido em hexano, a análise por cromatografia em fase gasosa utilizando padrões e através de espectrometria de massas permitiu a identificação de: esqualeno, tetradecano, hexadecano, campesterol, estigmasterol e beta-sitosterol; os ácidos graxos hexadecanóico, 9-octadecenóico e octadecanóico (majoritários; e decanóico, eicosanóico, docosanóico e tetracosanóico (minoritários. Cumarina foi isolada do extrato em acetato de etila e identificada por técnicas de RMN.Copaifera L. is one of the most economically important plant genera in the Amazon Region, since it exudes a resin-oil named copaiba oil possessing several confirmed pharmacological properties. In spite of that, phytochemical studies of the seeds from this genus are rare. Copaifera officinalis L. was the first species in the genus Copaifera to be described. This paper describes the chemical composition of the hexane and ethyl acetate extracts of the seeds from this species. In the hexane extract, gas chromatography and mass spectrometry analyses enabled us to identify squalene, tetradecane, hexadecane, campesterol, stigmasterol and beta-sitosterol; hexadecanoic, 9-octadecenoic and octadecanoic acids (major substances; as well as decanoic, eicosanoic, docosanoic and tetracosanoic (minor substances acids. Coumarin was detected in the ethyl acetate extract, isolated and identified by NMR.

  13. Hypersensitivity to Parietaria officinalis pollen in newcomers to the area with the plant.

    Science.gov (United States)

    Cvitanović, S; Marusić, M; Juricić, M; Vrdoljak, E; Petrovecki, M; Rozga, A; Stavljenić-Rukavina, A

    1993-11-01

    Hypersensitivity to Parietaria officinalis (wall pellitory) pollen and other environmental allergens was studied in pollinosis patients allergic to P. officinalis pollen who were born in areas without P. officinalis and later moved to the city of Split, where P. officinalis is responsible for some 65% of pollinosis cases. Highly significant positive correlations were found for both the intensity of skin test reaction and concentration of specific serum IgE with the length of residence in the area. In contrast, the respective data on subjects hypersensitive to P. officinalis pollen allergen, but born and living in the area of Split, revealed a tendency to negative correlation between age and intensity of hypersensitivity to P. officinalis. A number of patients from both groups were tested for presence of serum IgE antibodies specific for 14 common environmental allergens. Hypersensitivity to P. officinalis pollen was associated with hypersensitivity to olive, mugwort, and birch pollen in newcomers; hypersensitivity to birch and, to some extent, olive pollen was significantly more frequent in newcomers than in autochthonous patients who were allergic to P. officinalis pollen. Regardless of whether the patients were autochthons or newcomers to the area with P. officinalis, hypersensitivity to P. officinalis mostly excluded hypersensitivity to Dermatophagoides farinae and D. pteronyssinus, and vice versa.

  14. Importance of the pluripotency factor LIN28 in the mammalian nucleolus during early embryonic development.

    Science.gov (United States)

    Vogt, Edgar J; Meglicki, Maciej; Hartung, Kristina Ilka; Borsuk, Ewa; Behr, Rüdiger

    2012-12-01

    The maternal nucleolus is required for proper activation of the embryonic genome (EGA) and early embryonic development. Nucleologenesis is characterized by the transformation of a nucleolar precursor body (NPB) to a mature nucleolus during preimplantation development. However, the function of NPBs and the involved molecular factors are unknown. We uncover a novel role for the pluripotency factor LIN28, the biological significance of which was previously demonstrated in the reprogramming of human somatic cells to induced pluripotent stem (iPS) cells. Here, we show that LIN28 accumulates at the NPB and the mature nucleolus in mouse preimplantation embryos and embryonic stem cells (ESCs), where it colocalizes with the nucleolar marker B23 (nucleophosmin 1). LIN28 has nucleolar localization in non-human primate (NHP) preimplantation embryos, but is cytoplasmic in NHP ESCs. Lin28 transcripts show a striking decline before mouse EGA, whereas LIN28 protein localizes to NPBs at the time of EGA. Following knockdown with a Lin28 morpholino, the majority of embryos arrest between the 2- and 4-cell stages and never develop to morula or blastocyst. Lin28 morpholino-injected embryos arrested at the 2-cell stage were not enriched with nucleophosmin at presumptive NPB sites, indicating that functional NPBs were not assembled. Based on these results, we propose that LIN28 is an essential factor of nucleologenesis during early embryonic development.

  15. sel-11 and cdc-42, two negative modulators of LIN-12/Notch activity in C. elegans.

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    Min Sung Choi

    Full Text Available BACKGROUND: LIN-12/Notch signaling is important for cell-cell interactions during development, and mutations resulting in constitutive LIN-12/Notch signaling can cause cancer. Loss of negative regulators of lin-12/Notch activity has the potential for influencing cell fate decisions during development and the genesis or aggressiveness of cancer. METHODOLOGY/PRINCIPAL FINDINGS: We describe two negative modulators of lin-12 activity in C. elegans. One gene, sel-11, was initially defined as a suppressor of a lin-12 hypomorphic allele; the other gene, cdc-42, is a well-studied Rho GTPase. Here, we show that SEL-11 corresponds to yeast Hrd1p and mammalian Synoviolin. We also show that cdc-42 has the genetic properties consistent with negative regulation of lin-12 activity during vulval precursor cell fate specification. CONCLUSIONS/SIGNIFICANCE: Our results underscore the multiplicity of negative regulatory mechanisms that impact on lin-12/Notch activity and suggest novel mechanisms by which constitutive lin-12/Notch activity might be exacerbated in cancer.

  16. Fetal deficiency of lin28 programs life-long aberrations in growth and glucose metabolism.

    Science.gov (United States)

    Shinoda, Gen; Shyh-Chang, Ng; Soysa, T Yvanka de; Zhu, Hao; Seligson, Marc T; Shah, Samar P; Abo-Sido, Nora; Yabuuchi, Akiko; Hagan, John P; Gregory, Richard I; Asara, John M; Cantley, Lewis C; Moss, Eric G; Daley, George Q

    2013-08-01

    LIN28A/B are RNA binding proteins implicated by genetic association studies in human growth and glucose metabolism. Mice with ectopic over-expression of Lin28a have shown related phenotypes. Here, we describe the first comprehensive analysis of the physiologic consequences of Lin28a and Lin28b deficiency in knockout (KO) mice. Lin28a/b-deficiency led to dwarfism starting at different ages, and compound gene deletions showed a cumulative dosage effect on organismal growth. Conditional gene deletion at specific developmental stages revealed that fetal but neither neonatal nor adult deficiency resulted in growth defects and aberrations in glucose metabolism. Tissue-specific KO mice implicated skeletal muscle-deficiency in the abnormal programming of adult growth and metabolism. The effects of Lin28b KO could be rescued by Tsc1 haplo-insufficiency in skeletal muscles. Our data implicate fetal expression of Lin28a/b in the regulation of life-long effects on metabolism and growth, and demonstrate that fetal Lin28b acts at least in part via mTORC1 signaling.

  17. Isolation and Purification Chemical Compound from Melissa officinalis

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    Jamal A Nasser

    2014-01-01

    Full Text Available A chemical study was done on the leaves of Melissa officinalis. The separations of the chemical components were carried out by different chromatographic techniques and their structures were elucidated by spectroscopic method including nuclear magnetic resonance as well as gas chromatography-mass spectrometry. Three compounds were isolated and identified as friedoolean-7-one , stigmasterol and betulinic acid.

  18. Emblica officinalis (Amla: A review of potential therapeutic applications

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    Prasan R Bhandari

    2012-01-01

    Full Text Available Emblica officinalis Gaertn. or Phyllanthus emblica Linn, commonly known as Indian gooseberry or Amla, is perhaps the most important medicinal plant in the Indian traditional system of medicine, the Ayurveda. Several parts of the plant are used to treat a variety of diseases, but the most important is the fruit. Many ailments are treated by the fruit which is used either alone or in combination with other plants. These include common cold and fever; as a diuretic, laxative, liver tonic, refrigerant, stomachic, restorative, alterative, antipyretic, anti-inflammatory, hair tonic; to prevent peptic ulcer and dyspepsia, and as a digestive. E. officinalis possesses antipyretic, analgesic, antitussive, antiatherogenic, adaptogenic, cardioprotective, gastroprotective, antianemic, antihypercholesterolemic, wound healing, antidiarrheal, antiatherosclerotic, hepatoprotective, nephroprotective, and neuroprotective properties as demonstrated in numerous preclinical studies. Furthermore, experimental studies have reported that E. officinalis and some of its phytochemicals also exhibit anticarcinogenic properties. E. officinalis is also reported to possess radiomodulatory, chemomodulatory, chemopreventive, free radical scavenging, antioxidant, anti-inflammatory, antimutagenic and immunomodulatory activities. These properties are efficacious in the treatment and prevention of cancer. This review summarizes the results related to these properties and also emphasizes the aspects that warrant future research establishing its activity and utility as a cancer preventive and therapeutic drug in humans.

  19. A new heterocyclic compound from Cyathula officinalis Kuan

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    A new heterocyclic compound,named 5,5'-diisobutoxy-2,2'-bifuran(1),and four known compounds(2-5) were isolated from the roots of Cyathula officinalis Kuan.Their structures were elucidated by spectroscopic methods.Among these compounds,5,5'- dibutoxy-2,2'-bifuran(2) was isolated for the first time from this plant.

  20. Methyl carnosate, an antibacterial diterpene isolated from Salvia officinalis leaves.

    Science.gov (United States)

    Climati, Elisa; Mastrogiovanni, Fabio; Valeri, Maria; Salvini, Laura; Bonechi, Claudia; Mamadalieva, Nilufar Zokirzhonovna; Egamberdieva, Dilfuza; Taddei, Anna Rita; Tiezzi, Antonio

    2013-04-01

    Ethanolic extracts of Salvia officinalis leaves demonstrated antibacterial activity against Bacillus cereus. Fractionation of the extracts led to the isolation of the most active antibacterial compound, which, from spectroscopic and LC-MS evidence, was proved to be the diterpene, methyl carnosate.

  1. Two New Triterpenoids from the Roots of Sanguisorba officinalis L.

    Institute of Scientific and Technical Information of China (English)

    Fan ZHANG; Tie-Jun FU; Shu-Lin PENG; Zhong-Rong LIU; Li-Sheng DING

    2005-01-01

    Two new triterpenoids, octanordammar- 1,11,13(17)-trien- 17-ol-3,16-dione (1) and lup- 12-en- 15α,19β-diol-3,11-dioxo-28-oic acid (4), as well as 13 known compounds were isolated from the roots of Sanguisorba officinalis L. (Rosaceae). Their structures were determined using spectroscopic methods.

  2. Potential of Calendula alata for phytoremediation of stable cesium and lead from solutions.

    Science.gov (United States)

    Borghei, Mehdi; Arjmandi, Reza; Moogouei, Roxana

    2011-10-01

    Calendula alata plants were tested for their potential to remove stable cesium and lead from solutions in a 15-day period. The plants were grown hydroponically and placed in solutions containing CsCl and Pb(C₂H₃O₂)₂ at different concentrations (0.6, 2 and 5 mg l⁻¹). When plants were incubated in CsCl solutions 46.84 ± 2.12%, 41.35 ± 1.59%, and 52.06 ± 1.02% cesium was found to be remediated after 15 days. Moreover, more than 99% lead was removed from the Pb(C₂H₃O₂)₂ solution in all three concentrations after 15 days during the same period. When both CsCl and Pb(C₂H₃O₂)₂ were supplemented together in the solution, 9.92 ± 1.22%, 45.56 ± 3.52%, and 46.16 ± 1.48% cesium and 95.30 ± 0.72%, 96.64 ± 0.30%, and 99.02 ± 0.04% lead were removed after 15 days. The present study suggests that hydroponically grown C. alata could be used as a potential candidate plant for phytoremediation of cesium and lead from solutions; however, plants were found to be more efficient for the remediation of lead than cesium.

  3. The TRIM-NHL protein LIN-41 controls the onset of developmental plasticity in Caenorhabditis elegans.

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    Cristina Tocchini

    2014-08-01

    Full Text Available The mechanisms controlling cell fate determination and reprogramming are fundamental for development. A profound reprogramming, allowing the production of pluripotent cells in early embryos, takes place during the oocyte-to-embryo transition. To understand how the oocyte reprogramming potential is controlled, we sought Caenorhabditis elegans mutants in which embryonic transcription is initiated precociously in germ cells. This screen identified LIN-41, a TRIM-NHL protein and a component of the somatic heterochronic pathway, as a temporal regulator of pluripotency in the germline. We found that LIN-41 is expressed in the cytoplasm of developing oocytes, which, in lin-41 mutants, acquire pluripotent characteristics of embryonic cells and form teratomas. To understand LIN-41 function in the germline, we conducted structure-function studies. In contrast to other TRIM-NHL proteins, we found that LIN-41 is unlikely to function as an E3 ubiquitin ligase. Similar to other TRIM-NHL proteins, the somatic function of LIN-41 is thought to involve mRNA regulation. Surprisingly, we found that mutations predicted to disrupt the association of LIN-41 with mRNA, which otherwise compromise LIN-41 function in the heterochronic pathway in the soma, have only minor effects in the germline. Similarly, LIN-41-mediated repression of a key somatic mRNA target is dispensable for the germline function. Thus, LIN-41 appears to function in the germline and the soma via different molecular mechanisms. These studies provide the first insight into the mechanism inhibiting the onset of embryonic differentiation in developing oocytes, which is required to ensure a successful transition between generations.

  4. Oxidative stress modulation by Rosmarinus officinalis in CCl4-induced liver cirrhosis.

    Science.gov (United States)

    Gutiérrez, Rosalinda; Alvarado, José L; Presno, Manuel; Pérez-Veyna, Oscar; Serrano, Carmen J; Yahuaca, Patricia

    2010-04-01

    Rosmarinus officinalis (Lamiaceae) possesses antioxidant activity and hepatoprotective effects, and so may provide a possible therapeutic alternative for chronic liver disease. The effect produced by a methanolic extract of Rosmarinus officinalis on CCl(4)-induced liver cirrhosis in rats was investigated using both prevention and reversion models. Over the course of the development of cirrhosis, the increased enzymatic activities of gamma-glutamyl transpeptidase and alanine aminotransferase, and the rise in bilirubin levels caused by CCl(4) administration, were prevented by Rosmarinus officinalis co-administration. When the cirrhosis by oxidative stress was evaluated as an increase on liver lipoperoxidation, total lipid peroxides, nitric oxide in serum, and loss of erythrocyte plasma membrane stability, R. officinalis was shown to prevent such alterations. On cirrhotic animals treated with CCl(4), histological studies showed massive necrosis, periportal inflammation and fibrosis which were modified by R. officinalis. These benefits on experimental cirrhosis suggest a potential therapeutic use for R. officinalis as an alternative for liver cirrhosis.

  5. Ameliorative effects of thyme and calendula extracts alone or in combination against aflatoxins-induced oxidative stress and genotoxicity in rat liver.

    Science.gov (United States)

    Abdel-Aziem, Sekena H; Hassan, Aziza M; El-Denshary, Ezzeldein S; Hamzawy, Mohamed A; Mannaa, Fathia A; Abdel-Wahhab, Mosaad A

    2014-05-01

    The aims of the current work were to evaluate the hepatoprotective effect of calendula flowers and/or thyme leave extracts on aflatoxins (AFs)-induced oxidative stress, genotoxicity and alteration of p53 bax and bcl2 gene expressions. Eighty male Sprague-Dawley rats were divided into eight equal groups including: the control group, the group fed AFs-contaminated diet (2.5 mg/kg diet) for 5 weeks, the groups treated orally with thyme and/or calendula extract (0.5 g/kg b.w) for 6 weeks and the groups pretreated orally with thyme and/or calendula extract 1 week before and during AFs treatment for further 5 weeks. Blood, liver and bone marrow samples were collected for biochemical analysis, gene expression, DNA fragmentation and micronucleus assay. The results showed that AFs induced significant alterations in oxidative stress markers, increased serum AFP and inflammatory cytokine, percentage of DNA fragmentation, the expression of pro-apoptotic gene p53 and bax accompanied with a decrease in the expression of bcl2. Animals treated with the extracts 1 week before AFs treatment showed a significant decrease in oxidative damage markers, micronucleated cells, DNA fragmentation and modulation of the expression of pro-apoptotic genes. These results suggested that both calendula and thyme extracts had anti-genotoxic effects due to their higher content of total phenolic compounds.

  6. Bacterial diversity and real-time PCR based assessment of linA and linB gene distribution at hexachlorocyclohexane contaminated sites.

    Science.gov (United States)

    Lal, Devi; Jindal, Swati; Kumari, Hansi; Jit, Simran; Nigam, Aeshna; Sharma, Pooja; Kumari, Kirti; Lal, Rup

    2015-03-01

    The disposal of hexachlorocyclohexane (HCH) muck has created large number of HCH dumpsites all over the world from where the harmful HCH isomers are leaking into the environment. Bacteria have evolved at such contaminated sites that have the ability to degrade HCH. Degradation of various HCH isomers in bacterial strains is mediated primarily by two genes: linA and linB which encode dehydrochlorinase and haloalkane dehalogenase respectively. In this study we explored one such highly contaminated HCH dumpsite located in Lucknow, Uttar Pradesh, India. To assess the biostimulation potential of the contaminated site, microbial diversity study and real-time PCR based quantification of lin genes was carried out. The soil samples from dumpsite and surrounding areas were found to be highly contaminated with HCH residue levels as high as 1.8 × 10(5)  mg kg(-1). The residues were detected in areas upto 13 km from the dumpsite. Sphingomonads, Chromohalobacter, and Marinobacter were the dominant genera present at the dump-site. Role of Sphingomonads in HCH degradation has been well documented. The highest copy numbers of linA and linB genes as determined using real-time PCR were 6.2 × 10(4) and 5.3 × 10(5), respectively, were found in sample from the dump site. The presence of Sphingomonads, linA, and linB genes from HCH contaminated soil indicates the presence of indigenous bacterial communities capable of HCH degradation.

  7. Neuroprotective effect of Rosmarinus officinalis extract on human dopaminergic cell line, SH-SY5Y.

    Science.gov (United States)

    Park, Se-Eun; Kim, Seung; Sapkota, Kumar; Kim, Sung-Jun

    2010-07-01

    Hydrogen peroxide (H2O2) is a major Reactive Oxygen Species (ROS), which has been implicated in many neurodegenerative conditions including Parkinson's disease (PD). Rosmarinus officinalis (R. officinalis) has been reported to have various pharmacological properties including anti-oxidant activity. In this study, we investigated the neuroprotective effects of R. officinalis extract on H2O2-induced apoptosis in human dopaminergic cells, SH-SY5Y. Our results showed that H2O2-induced cytotoxicity in SH-SY5Y cells was suppressed by treatment with R. officinalis. Moreover, R. officinalis was very effective in attenuating the disruption of mitochondrial membrane potential and apoptotic cell death induced by H2O2. R. officinalis extract effectively suppressed the up-regulation of Bax, Bak, Caspase-3 and -9, and down-regulation of Bcl-2. Pretreatment with R. officinalis significantly attenuated the down-regulation of tyrosine hydroxylase (TH), and aromatic amino acid decarboxylase (AADC) gene in SH-SY5Y cells. These findings indicate that R. officinalis is able to protect the neuronal cells against H2O2-induced injury and suggest that R. officinalis might potentially serve as an agent for prevention of several human neurodegenerative diseases caused by oxidative stress and apoptosis.

  8. The role of Lin28b in myeloid and mast cell differentiation and mast cell malignancy.

    Science.gov (United States)

    Wang, L D; Rao, T N; Rowe, R G; Nguyen, P T; Sullivan, J L; Pearson, D S; Doulatov, S; Wu, L; Lindsley, R C; Zhu, H; DeAngelo, D J; Daley, G Q; Wagers, A J

    2015-06-01

    Mast cells (MCs) are critical components of the innate immune system and important for host defense, allergy, autoimmunity, tissue regeneration and tumor progression. Dysregulated MC development leads to systemic mastocytosis (SM), a clinically variable but often devastating family of hematologic disorders. Here we report that induced expression of Lin28, a heterochronic gene and pluripotency factor implicated in driving a fetal hematopoietic program, caused MC accumulation in adult mice in target organs such as the skin and peritoneal cavity. In vitro assays revealed a skewing of myeloid commitment in LIN28B-expressing hematopoietic progenitors, with increased levels of LIN28B in common myeloid and basophil-MC progenitors altering gene expression patterns to favor cell fate choices that enhanced MC specification. In addition, LIN28B-induced MCs appeared phenotypically and functionally immature, and in vitro assays suggested a slowing of MC terminal differentiation in the context of LIN28B upregulation. Finally, interrogation of human MC leukemia samples revealed upregulation of LIN28B in abnormal MCs from patients with SM. This work identifies Lin28 as a novel regulator of innate immune function and a new protein of interest in MC disease.

  9. The C. elegans hox gene lin-39 controls cell cycle progression during vulval development.

    Science.gov (United States)

    Roiz, Daniel; Escobar-Restrepo, Juan Miguel; Leu, Philipp; Hajnal, Alex

    2016-10-01

    Cell fate specification during organogenesis is usually followed by a phase of cell proliferation to produce the required number of differentiated cells. The Caenorhabditis elegans vulva is an excellent model to study how cell fate specification and cell proliferation are coordinated. The six vulval precursor cells (VPCs) are born at the first larval stage, but they arrest in the G1 phase of the cell cycle until the beginning of the third larval stage, when their fates are specified and the three proximal VPCs proliferate to generate 22 vulval cells. An epidermal growth factor (EGF) signal from the gonadal anchor cell combined with lateral DELTA/NOTCH signaling between the VPCs determine the primary (1°) and secondary (2°) fates, respectively. The hox gene lin-39 plays a key role in integrating these spatial patterning signals and in maintaining the VPCs as polarized epithelial cells. Using a fusion-defective eff-1(lf) mutation to keep the VPCs polarized, we find that VPCs lacking lin-39 can neither activate lateral NOTCH signaling nor proliferate. LIN-39 promotes cell cycle progression through two distinct mechanisms. First, LIN-39 maintains the VPCs competent to proliferate by inducing cdk-4 cdk and cye-1 cyclinE expression via a non-canonical HOX binding motif. Second, LIN-39 activates in the adjacent VPCs the NOTCH signaling pathway, which promotes VPC proliferation independently of LIN-39. The hox gene lin-39 is therefore a central node in a regulatory network coordinating VPC differentiation and proliferation.

  10. Identification of microRNAs regulating reprogramming factor LIN28 in embryonic stem cells and cancer cells.

    Science.gov (United States)

    Zhong, Xiaomin; Li, Ning; Liang, Shun; Huang, Qihong; Coukos, George; Zhang, Lin

    2010-12-31

    LIN28 (a homologue of the Caenorhabditis elegans lin-28 gene) is an evolutionarily conserved RNA-binding protein and a master regulator controlling the pluripotency of embryonic stem cells. Together with OCT4, SOX2, and NANOG, LIN28 can reprogram somatic cells, producing induced pluripotent stem cells. Expression of LIN28 is highly restricted to embryonic stem cells and developing tissues. In human tumors, LIN28 is up-regulated and functions as an oncogene promoting malignant transformation and tumor progression. However, the mechanisms of transcriptional and post-transcriptional regulation of LIN28 are still largely unknown. To examine microRNAs (miRNAs) that repress LIN28 expression, a combined in silico prediction and miRNA library screening approach was used in the present study. Four miRNAs directly regulating LIN28 (let-7, mir-125, mir-9, and mir-30) were initially identified by this approach and further validated by quantitative RT-PCR, Western blot analysis, and a LIN28 3'-UTR reporter assay. We found that expression levels of these four miRNAs were clustered together and inversely correlated with LIN28 expression during embryonic stem cell differentiation. In addition, the expression of these miRNAs was remarkably lower in LIN28-positive tumor cells compared with LIN28-negative tumor cells. Importantly, we demonstrated that these miRNAs were able to regulate the expression and activity of let-7, mediated by LIN28. Taken together, our studies demonstrate that miRNAs let-7, mir-125, mir-9, and mir-30 directly repress LIN28 expression in embryonic stem and cancer cells. Global down-regulation of these miRNAs may be one of the mechanisms of LIN28 reactivation in human cancers.

  11. Chemical composition of Rosmarinus officinalis essential oil and antioxidant action against gastric damage induced by absolute ethanol in the rat

    Directory of Open Access Journals (Sweden)

    Christiane Takayama

    2016-08-01

    Conclusions: We suggest that the monoterpenes present in the essential oil obtained from R. officinalis may be among the active principles responsible for the antioxidant activity shown by essential oil of R. officinalis.

  12. Antiviral compounds and one new iridoid glycoside from Cornus officinalis

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    From Cornus officinalis Sieb. et Zucc., bioassay-guided fractionation led to the isolation of four active tannin compounds with high effectiveness of inhibiting Hepatitis C virus NS3 serine protease in vitro. The compounds are: 1, 2, 3, 6-tetragalloyl-β-D-glucopyranose ( 1 ), 1, 2, 3, 4, 6- pentagalloyl-β-D-glucopyranose (2), Tellimagrandin Ⅰ (3) and Tellimagrandin Ⅱ (4). The four compounds could inhibit HCV NS3 protease in vitro with IC50 values of 6.98, 5.11, 7.0 and 4.8 μmol/L respectively. In addition, a new iridoid glycoside (5) was also isolated from Cornus officinalis Sieb. et Zucc., which was assigned to be 7-O-butyl morroniside by spectroscopic analysis.

  13. Determination Of Antioxidant Activities In Freshliver (Salvia Officinalis) Plant

    OpenAIRE

    Arıduru, Rana; Arabacı, Gülnur

    2013-01-01

    In this study, we determined the antioxidant activities of four different solvent fractions (ethanol, methanol, acetone and ethyl acetate) obtained from Freshliver plant leaves (Salvia officinalis) by employing two different assays such as 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) and Folin-Ciocaltaeu method. The results showed that ethanolextract of freshliver plant exhibited the highest total phenolic contents (43.55 mg GAE/g extract), followed by methanol-extract of freshliver plant (23...

  14. Self-Similar Solutions to the Lin-Reissner-Tsien Equation%Lin- Reissner-Tsien方程的自相似解

    Institute of Scientific and Technical Information of China (English)

    J·郝泽曼; K·法拉菲路; R·A·冯歌德; 黄雅意

    2011-01-01

    The Lin-Reissner-Tsien equation describes unsteady transonic flows under the transonic approximation. The equation was reduced to an ordinary differential equation via a similarity transformation. The resulting equation was then solved analytically, and in some cases even exactly. Numerical simulations were provided for the cases in which there were no exact solutions. Traveling wave solutions were also obtained.%Lin-Reissner-Tsien方程描述了在跨音速近似下的不稳定跨音速流动.通过相似变换,将Lin-Reissner-Tsien方程简化为一个常微分方程.然后解析求解所得到的方程,并在某些情况下得到的正好是精确解.上述情况下无法得到精确解时,给出了数值模拟.还得到了行波解.

  15. PHYTOCHEMICAL AND TOXICITY STUDY OF EMBLICA OFFICINALIS (AMLA

    Directory of Open Access Journals (Sweden)

    Pandey Govind

    2011-03-01

    Full Text Available In the present study, phytochemistry and toxicities (acute and chronic of Emblica officinalis fruit (Amla have been determined. The hydroalcoholic extract (HAE of amla was prepared and its extractability was found to be 46.9%. Different chemical tests showed the presence of various active principles or phytoconstituents, viz., alkaloids, glycosides, reducing sugars, tannins, resins, saponins, sterols and fixed oils. For acute toxicity, including median lethal dose (LD50 of amla, its HAE was administered @ 250, 500 and 1000 mg/kg body weight to female albino rats of groups 2 to 4, respectively. Rats of group 1 were given normal saline to serve as control. There was no mortality up to 48 hr, hence this drug showed the LD50 above 1000 mg/kg. For chronic toxicity of E. officinalis HAE, similar drug dosage schedule was applied in groups 1 to 4 of rats as used for acute toxicity study; however, the drug was given for 3 weeks. During this period, E. officinalis did not cause any untoward effect.

  16. Subcellular effects of drought stress in Rosmarinus officinalis.

    Science.gov (United States)

    Olmos, E; Sánchez-Blanco, M J; Ferrández, T; Alarcón, J J

    2007-01-01

    The use of Rosmarinus officinalis, and other wild plant species, in the Mediterranean area is an interesting solution in order to avoid the desertification and rapid soil erosion, because of their good resistance to environmental conditions. Previous articles have described experiments designed to determine the impact of water stress at the plant level in this species, but more knowledge is required at the subcellular and ultrastructural levels. An anatomic and ultrastructural study of the leaves was conducted on Rosmarinus officinalis plants growing under different water treatments. In the leaves of water-stressed plants, the leaf water potential and turgor decreased, and leaf osmotic potential became more negative with respect to control plants. The anatomic investigations showed that both the mesophyll intercellular spaces and the epidermal cell size were reduced significantly under the more intense drought stress conditions. At the subcellular level, chloroplasts accumulated plastoglobuli and lipid bodies, and cuticle thickness was increased under water stress. In our experiment, the anatomic and ultrastructural modifications of Rosmarinus officinalis could be considered an additional adaptation to drought stress together with physiological and biochemical modifications as antioxidant accumulation.

  17. Another Security Improvement over the Lin et al.'s E-voting Scheme

    Science.gov (United States)

    Asaar, Maryam Rajabzadeh; Mohajeri, Javad; Salmasizadeh, Mahmoud

    In 2003, Lin et al. have proposed an electronic voting scheme which can be utilized in large-scale elections, and claimed it detects double voting. But in this paper, by presenting an attack, we show that voters can successfully vote more than once without being detected. Hence, we propose a new modified scheme based on the Lin et al.'s scheme with the same efficiency to solve this weakness and analyze its security.

  18. In vitro effects of Salvia officinalis L. essential oil on Candida albicans

    Directory of Open Access Journals (Sweden)

    Tularat Sookto

    2013-05-01

    Conclusions: S. officinalis L. essential oil exhibited anticandidal activities against C. albicans and had inhibitory effects on the adhesion of the cells to PMMA resin surface. With further testing and development, S. officinalis essential oil may be used as an antifungal denture cleanser to prevent candidal adhesion and thus reduce the risk of candida-associated denture stomatitis.

  19. Lin-Kernighan Heuristic Adaptation for the Generalized Traveling Salesman Problem

    CERN Document Server

    Karapetyan, Daniel

    2010-01-01

    Lin-Kernighan heuristic is known to be one of the most successful heuristics for the Traveling Salesman Problem (TSP). It has also proven its efficiency in application to some other problems. However, it was never applied to the the Generalized Traveling Salesman Problem (GTSP) though it has the same nature as TSP. In this paper we discuss possible adaptations of TSP heuristics for GTSP and focus on the case of Lin-Kernighan algorithm. At first, we provide an easy-to-understand description of the original Lin-Kernighan heuristic. Then we propose several adaptations, both trivial and complicated ones. Finally, we conduct a fair competition between all the variations of Lin-Kernighan adaptation and some other GTSP heuristics. It appears that our adaptation of Lin-Kernighan algorithm for GTSP reproduces the success of the original heuristic. Different variations of our adaptation outperform all other heuristics in a wide range of tradeoffs between solution quality and running time, making Lin-Kernighan the state...

  20. Recent Trends in Potential Traditional Indian Herbs Emblica Officinalis and Its Medicinal Importance

    Directory of Open Access Journals (Sweden)

    Lokesh Deb

    2012-05-01

    Full Text Available Emblica Officinalis is a natural, efficacious, an antioxidant with the richest natural source of Vitamin C. Emblica Officinalis berries have the highest amount of naturally occurring vitamin C of any ripe fruit in the world used as a traditional food. Numerous studies conducted on Emblica Officinalis fruit suggest that it has anti-viral properties and also functions as an anti-bacterial and anti-fungal agent. The gelatinous plum-sized Amla fruit contains naturally occurring vitamin, heat stable vitamin C. A clinical study on patients with pulmonary tuberculosis showed that the vitamin C contained in Emblica Officinalis was better assimilated than synthetic vitamin C. Further research of contemporary and traditional medical literature indicates that Emblica Officinalis either in combination with other herbs or alone has been useful in the amelioration of colds, warts, skin afflictions, influenza, anemia, diabetes, lung conditions, elevated cholesterol and as an immune restorative in cancer conditions. It is one of the best natural antiageing remedies Emblica Officinalis is used in treatment of Acne and other skin problems. Emblica Officinalis is amazingly effective natural anti-ageing product. Emblica Officinalis is very effective in treatment of Acidity and Peptic ulcers. Emblica Officinalis is rich in Vitamin C, Calcium, Iron, essential amino acids and many other vitamins and minerals and anti-oxidants. Regular Use of Emblica Officinalis improves immunity, fights cancers, rejuvenates the body. It fights chronic diseases like hypertension, high Cholesterol, Diabetes, AIDS, influenza, Chronic cough and cold, Chronic infections, Chronic fatigue and Chronic inflammatory conditions. Ayurveda describes it as one of the best herbs for Diabetes, bleeding disorders, strength and stamina promoter.

  1. Metabolism of monoterpanes: metabolic fate of (+)-camphor in sage (Salvia officinalis). [Salvia officinalis

    Energy Technology Data Exchange (ETDEWEB)

    Croteau, R.; El-Bialy, H.; Dehal, S.S.

    1987-07-01

    The bicyclic monoterpene ketone (+)-camphor undergoes lactonization to 1,2-campholide in mature sage (Salvia officinalis L.) leaves followed by conversion to the ..beta..-D-glucoside-6-O-glucose ester of the corresponding hydroxy acid (1-carboxymethyl-3-hydroxy-2,2,3-trimethyl cyclopentane). Analysis of the disposition of (+)-(G-/sup 3/H)camphor applied to midstem leaves of intact flowering plants allowed the kinetics of synthesis of the bis-glucose derivative and its transport from leaf to root to be determined, and gave strong indication that the transport derivative was subsequently metabolized in the root. Root extracts were shown to possess ..beta..-glucosidase and acyl glucose esterase activities, and studies with (+)-1,2(U-/sup 14/C)campholide as substrate, using excised root segments, revealed that the terpenoid was converted to lipid materials. Localization studies confirmed the radiolabeled lipids to reside in the membranous fractions of root extracts, and analysis of this material indicated the presence of labeled phytosterols and labeled fatty acids (C/sub 14/ to C/sub 20/) of acyl lipids. Although it was not possible to detail the metabolic steps between 1,2-campholide and the acyl lipids and phytosterols derived therefrom because of the lack of readily detectable intermediates, it seemed likely that the monoterpene lactone was degraded to acetyl CoA which was reincorporated into root membrane components via standard acyl lipid and isoprenoid biosynthetic pathways. Monoterpene catabolism thus appears to represent a salvage mechanism for recycling mobile carbon from senescing oil glands on the leaves to the roots.

  2. A potential regulatory loop between Lin28B:miR-212 in androgen-independent prostate cancer

    Science.gov (United States)

    BORREGO-DIAZ, EMMA; POWERS, BENJAMIN C.; AZIZOV, VUGAR; LOVELL, SCOTT; REYES, RUBEN; CHAPMAN, BRADLEY; TAWFIK, OSSAMA; McGREGOR, DOUGLAS; DIAZ, FRANCISCO J.; WANG, XINKUN; VAN VELDHUIZEN, PETER

    2014-01-01

    Lin28 is a family of RNA binding proteins and microRNA regulators. Two members of this family have been identified: Lin28A and Lin28B, which are encoded by genes localized in different chromosomes but share a high degree of sequence identity. The role of Lin28B in androgen-independent prostate cancer (AIPC) is not well understood. Lin28B is expressed in all grades of prostatic carcinomas and prostate cancer cell lines, but not in normal prostate tissue. In this study we found that Lin28B co-localized in the nucleus and cytoplasm of the DU145 AIPC. The expression of Lin28B protein positively correlated with the expression of the c-Myc protein in the prostate cancer cell lines and silencing of Lin28B also correlated with a lower expression of the c-Myc protein, but not with the downregulation of c-Myc messenger RNA (mRNA) in the DU145 AIPC cells. We hypothesized that Lin28B regulates the expression of c-Myc protein by altering intermediate c-Myc suppressors. Therefore, a microRNA profile of DU145 cells was performed after Lin28B siRNA silencing. Nineteen microRNAs were upregulated and eleven microRNAs were downregulated. The most upregulated microRNAs were miR-212 and miR-2278. Prior reports have found that miR-212 is suppressed in prostate cancer. We then ran TargetScan software to find potential target mRNAs of miR-212 and miR-2278, and it predicted Lin28B mRNA as a potential target of miR-212, but not miR-2278. TargetScan also predicted that c-Myc mRNA is not a potential target of miR-212 or miR-2278. These observations suggest that Lin28B:miR-212 may work as a regulatory loop in androgen-independent prostate cancer. Furthermore, we report a predictive 2-fold symmetric model generated by the superposition of the Lin28A structure onto the I-TASSER model of Lin28B. This structural model of Lin28B suggests that it shows unique microRNA binding characteristics. Thus, if Lin28B were to bind miRNAs in a manner similar to Lin28A, conformational changes would be necessary

  3. Antidepressant effect of Melissa officinalis in the forced swimming test

    Directory of Open Access Journals (Sweden)

    M Emamghoreishi

    2009-03-01

    Full Text Available ABSTRACT Background: In Iranian and other traditional medicines, an antidepressant effect has been indicated for Melissa officinalis (Lamiaceae. However, studies showing its antidepressant effect is lacking. Therefore, the present study was undertaken to examine whether the aqueous extract and essential oil from leaves of Melissa officinalis have an antidepressant-like activity in mice.  Materials and Methods: The effect of subchronic administration of different doses of the aqueous extract (25, 75, 150, 300 mg/kg or water; n=9-10 and the essential oil (10, 25, 75, 150, 300 mg/kg or almond oil; n=9-10 on immobility, climbing, and swimming behaviors were evaluated in the forced swimming test. Fluoxetine (20mg/kg and imipramine (15 mg/kg were used as reference drugs. Additionally, the effect of both plant preparations on spontaneous activity was examined. Results: All doses of the aqueous extract, used in this study, produced a significant reduction in immobility along with an increase in climbing behavior which is similar to those which have been observed with imipramine. Essential oil caused a dose-dependent reduction in immobility and an increase in climbing at all studied doses, compared to control group. Only the highest dose (300mg/kg of essential oil showed a significant increase in swimming behavior. The aqueous extract, but not the essential oil, decreased spontaneous activity in a dose dependent manner. Conclusion: The results of this study suggests that the Melissa officinalis possess an antidepressant-like activity similar to imipramine which may have a potential clinical value for treatment of depression.

  4. Rosmarinus officinalis L. as cause of contact dermatitis.

    Science.gov (United States)

    Miroddi, M; Calapai, G; Isola, S; Minciullo, P L; Gangemi, S

    2014-01-01

    Because of the widespread use of botanicals, it has become crucial for health professionals to improve their knowledge about safety problems. Several herbal medicines contain chemicals with allergenic properties responsible for contact dermatitis. Among these, one is Rosmarinus officinalis L. (rosemary), a plant used since ancient times in folk medicine; at the present time it is used worldwide as a spice and flavouring agent, as a preservative and for medicinal and cosmetic purposes. The present article aims to revise and summarise scientific literature reporting cases of contact dermatitis caused by the use of R. officinalis as a raw material or as herbal preparations. Published case reports were researched on the following databases and search engines: PUBMED, MEDLINE, EMBASE, Google Scholar, Scopus. The used keywords were: R. officinalis and rosemary each alone or combined with the words allergy, contact dermatitis, allergic contact dermatitis, sensitisation and occupational dermatitis. The published case reports show that both rosemary extracts and raw material can be responsible for allergic contact dermatitis. Two cases related to contact dermatitis caused by cross-reactivity between rosemary and thyme were also commented. The diterpene carnosol, a chemical constituent of this plant, has been imputed as a common cause for this reaction. The incidence of contact dermatitis caused by rosemary is not common, but it could be more frequent with respect to the supposed occurrence. It seems plausible that cases of contact dermatitis caused by rosemary are more frequent with respect to the supposed occurrence, because they could be misdiagnosed. For this reason, this possibility should be carefully considered in dermatitis differential diagnosis.

  5. ANTIOXIDANT, IMMUNOMODULATORY AND ANTICANCER ACTIVITIES OF EMBLICA OFFICINALIS: AN OVERVIEW

    Directory of Open Access Journals (Sweden)

    Madhuri S.

    2011-08-01

    Full Text Available Emblica officinalis (Amla is traditionally used for several diseases, and it is believed to increase the defense against diseases. It is particularly used for the treatment of cancer, diabetes, liver disorders, heart disease, ulcer, snake venom, haemorrhage, diarrhea, dysentery, anaemia and ophthalmic disorders. The antioxidant, immunomodulatory, anticancer, cytoprotective, analgesic, antimicrobial, antipyretic, antitussive and gastroprotective are the important properties of amla. Vitamin C, tannins and flavaniods present in amla have very powerful antioxidant activities. Due to rich vitamin C, amla is successfully used in the treatment of human scurvy.

  6. Anti-Platelet Fraction Isolated from Galega Officinalis

    Directory of Open Access Journals (Sweden)

    Atanasov A.

    2016-10-01

    Full Text Available A fraction from crude extract of Galega officinalis has been purified by column chromatography on Sephadex G-25, Sepharose 4B, DEAE-Cellulose and Sephadex G-100. The final purification factor of the fraction is 120. The peak in elution profile after Sephadex G-150 shows a molecular weight of 100-140 kDa. The isolated fraction appears to have 74% polysaccharides and 23% of proteins. No loss of activity of the final fraction is observed after storage for several months at 4°C and in lyophilized condition. The fraction compounds inhibit platelet aggregation induced by ADP, collagen and thrombin.

  7. Determination Of Antioxidant Activities In Freshliver (Salvia Officinalis) Plant

    OpenAIRE

    Rana Arıduru; Gülnur Arabacı

    2013-01-01

    In this study, we determined the antioxidant activities of four different solvent fractions obtained from Freshliver plant leaves (Salvia officinalis) by employing two different assays such as 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) and Folin-Ciocaltaeu method. The results showed that ethanol-extract of freshliver plant exhibited the highest total phenolic contents mg GAE/g extract; 43,55, methanol-extract of 23.62, ethyl acetate extract 18.29, and acetone extract 11.58. All the extracti...

  8. Phenolic compounds from the roots of Valeriana officinalis var. latifolia

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Peng-Cheng; Ran, Xin-Hui; Luo, Huai-Rong; Liu, Yu-Qing; Zhou Jun [State Key Laboratory of Phytochemistry and Plant Resources in West China. Kunming Institute of Botany, Chinese Academy of Sciences (China); Ma, Qing-Yun; Zhao, You-Xing, E-mail: zhoujun3264@yahoo.com.cn, E-mail: zhaoyouxing@itbb.org.cn [Key Laboratory of Biology and Genetic Resources of Tropical Crops. Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology. Chinese Academy of Tropical Agriculture Sciences (China)

    2013-09-15

    A new benzofuran neolignan, dihydrodehydrodiconiferyl alcohol 9-isovalerate, along with ten known phenolic compounds, olivil, pinoresinol, 8-hydroxypinoresinol, pinorespiol, 8-hydroxy- 7-epipinoresinol, trans-p-hydroxyphenyl- propenoic acid, cis-p-hydroxyphenyl-propenoic acid, ferulic acid, isoferulic acid and isovanillin were isolated from the roots of Valeriana officinalis var. latifolia. Their structures and configurations were elucidated on the basis of spectroscopic methods. The inhibitory activity for acetylcholinesterase (AChE) and enhancing activity on nerve growth factor (NGF)-mediated neurite outgrowth in PC12 cells of dihydrodehydrodiconiferyl alcohol 9-isovaterate and olivil were evaluated. (author)

  9. A cell-specific enhancer that specifies lin-3 expression in the C. elegans anchor cell for vulval development.

    Science.gov (United States)

    Hwang, Byung Joon; Sternberg, Paul W

    2004-01-01

    During C. elegans vulval development, the anchor cell (AC) in the somatic gonad expresses lin-3, activating the EGF receptor signaling pathway in vulval precursor cells (VPCs) and thereby inducing and patterning VPCs. Previous studies with lin-3 mutants and transgene expression have revealed that the level of LIN-3 in the AC must be precisely regulated for proper vulval development. To understand how lin-3 expression is achieved in the AC, we identified a 59 bp lin-3 enhancer sufficient to activate lin-3 transcription solely in the AC. The enhancer contains two E-box elements, and one FTZ-F1 nuclear hormone receptor (NHR) binding site that is mutated in a vulvaless mutant, lin-3(e1417). Mutagenesis studies show that both E-boxes and the NHR binding site are necessary to express lin-3 in the AC. In vitro DNA-binding studies and in vivo functional assays indicate that distinct trans-acting factors, including the E-protein/Daughterless homolog HLH-2 and unidentified nuclear hormone receptor(s), are necessary for lin-3 transcription in the AC and thus are involved in vulval development.

  10. νLIN6: An Integrated Mobility Protocol in IPv6

    Science.gov (United States)

    Banno, Ayumi; Teraoka, Fumio

    This paper proposes a protocol called νLIN6 which supports both network mobility and host mobility in IPv6. There are several proposals to support network mobility and host mobility. Network Mobility (NEMO) Basic Support Protocol has several problems such as pinball routing, large header overhead due to multiple levels of tunneling, and a single point of failure. Optimized NEMO (ONEMO) and Mobile IP with Address Translation (MAT) are solutions to provide route optimization, but they generate a lot of signaling messages at a handover. In νLIN6, packet relay is required only once regardless of the nested level in network mobility while optimal routing is always provided in host mobility. A fixedsized extension header is used in network mobility while there is no header overhead in host mobility. νLIN6 is more tolerant of network failure and mobility agent failure than NEMO Basic Support Protocol. It also allows ordinary IPv6 nodes to communicate with mobile nodes and nodes in the mobile network. We implemented νLIN6 on NetBSD 2.0 Release. Our measurement results showed νLIN6 can provide host mobility and network mobility with low overhead.

  11. Use of Systemic Rosmarinus Officinalis to Enhance the Survival of Random-Pattern Skin Flaps

    Science.gov (United States)

    İnce, Bilsev; Bilgen, Fatma; Gündeşlioğlu, Ayşe Özlem; Dadacı, Mehmet; Kozacıoğlu, Sümeyye

    2016-01-01

    Background Skin flaps are commonly used in soft-tissue reconstruction; however, necrosis can be a frequent complication. Several systemic and local agents have been used in attempts to improve skin flap survival, but none that can prevent flap necrosis have been identified. Aims This study aims to determine whether the use of systemic Rosmarinus officinalis (R. officinalis) extract can prevent flap necrosis and improve skin flap recovery. Study Design Animal experimentation. Methods Thirty-five Wistar albino rats were divided in five groups. A rectangular random-pattern flaps measuring 8×2 cm was elevated from the back of each rat. Group I was the control group. In Group II, 0.2 ml of R. officinalis oil was given orally 2h before surgery. R. officinalis oil was then applied orally twice a day for a week. In Group III, R. officinalis oil was given orally twice a day for one week before surgery. At the end of the week, 0.2 mL of R. officinalis oil was given orally 2 h before surgery. In Group IV, 0.2 mL of R. officinalis oil was injected subcutaneously 2 h before surgery. After the surgery, 0.2 mL R. officinalis oil was injected subcutaneously twice a day for one week. In Group V, 0.2 mL R. officinalis oil was injected subcutaneously twice a day for one week prior to surgery. At the end of the week, one last 0.2 mL R. officinalis oil injection was administered subcutaneously 2 h before surgery. After the surgery, 0.2 mL R. officinalis oil was injected subcutaneously twice a day for one week. Results The mean percentage of viable surface area was significantly greater (p<0.05) in Groups II, III, IV, and V as compared to Group I. Mean vessel diameter was significantly greater (p<0.05) in Groups II, III, IV, and V as compared to Group I. Conclusion We have determined that, in addition to its anti-inflammatory and anti-oxidant effects, R. officinalis has vasodilatory effects that contribute to increased skin flap survival. PMID:27994918

  12. The effect of Emblica officinalis diet on lifespan, sexual behavior, and fitness characters in Drosophila melanogaster.

    Science.gov (United States)

    Pathak, Pankaj; Prasad, B R Guru; Murthy, N Anjaneya; Hegde, S N

    2011-04-01

    Drosophila is an excellent organism to test Ayurvedic medicines. The objective of our study was to explore the potential of Emblica officinalis drug on longevity, sexual behavior, and reproductive fitness of Drosophila melanogaster using adult feeding method. Increase in the lifespan, fecundity, fertility, ovarioles number, and developmental time was observed in both parents and F1 generation, but not in the F2 generation in experimental culture (control + E. officinalis). According to the Duncan's multiple range test and ANOVA, there is a significant difference between two cultures. It was also noticed that E. officinalis influence some fitness characters in Drosophila along with sexual behavior.

  13. Lin Zhichun's Contributions to the Study of Ancient World History in China

    Institute of Scientific and Technical Information of China (English)

    Yan; Shaoxiang

    2015-01-01

    Lin Zhichun (林志纯, 1910-2007, henceforthreferred to as Lin, but popularly known by hispen name of Ri Zhi), was a famous historian ofancient world history in China. Born in Fujian,he was enrolled in The Great China Universityin 1939 as a junior college student, and becamean undergraduate in 1941. Lin probably knewwhat he would do after graduation and learntEnglish, Russian and Latin in the university.1But the turmoil in China in the next 10 years(Japanese invasion of China since 1937 and thenthe Liberation War) made his life rather hard andhe had to move between Shanghai, Beijing and Shenyang successively in order tosupport himself. However, he tried to carry out his researches on Chinese history andpublished some articles since 1934.

  14. Run-time system based on LinSERCANS and Soft-PLC

    Institute of Scientific and Technical Information of China (English)

    Cunfeng KANG; Chong WANG; Chunmin MA; Xudong HUANG; Renyuan FEI

    2009-01-01

    Soft-PLC with open architecture is the direction of development in industrial automation. This paper discusses the method of communication between the interface functions of LinSERCANS under RTLinux and the external library of Soft-PLC under Windows. Based on the API HOOK theory, the communication between the interface functions of LinSERCANS and the external libraries of Soft-PLC is set up and it solves the calling functions of dynamic link libraries in different operation systems. It is able to combine LinSERCANS with the Soft-PLC, and a run-time system is developed based on the interface technology of the serial real-time communication system (SERCOS) and technology of soft-PLC. This run-time system has been used in all electronic injection molding machines and works very well.

  15. Mortier de fibres de lin : applications possibles et caractérisation

    OpenAIRE

    Jurkiewiez B.; Si Larbi A.; Hamelin P.

    2011-01-01

    Nous présentons les résultats d’une étude sur des mortiers renforcés de fibres de lin. Une brève étude bibliographique montre notamment que le lin en tant que renfort a fait l’objet de peu de travaux malgré une disponibilité importante, un coût peu élevé et de bonnes propriétés spécifiques. Nous montrons ensuite que, du point de vue de la mise en oeuvre et du comportement mécanique, les conditionnements de la fibre de lin sous forme de fibres courtes ou de grilles apparaisse...

  16. Promotion of hair growth by Rosmarinus officinalis leaf extract.

    Science.gov (United States)

    Murata, Kazuya; Noguchi, Kazuma; Kondo, Masato; Onishi, Mariko; Watanabe, Naoko; Okamura, Katsumasa; Matsuda, Hideaki

    2013-02-01

    Topical administration of Rosmarinus officinalis leaf extract (RO-ext, 2 mg/day/mouse) improved hair regrowth in C57BL/6NCrSlc mice that experienced hair regrowth interruption induced by testosterone treatment. In addition, RO-ext promoted hair growth in C3H/He mice that had their dorsal areas shaved. To investigate the antiandrogenic activity mechanism of RO-ext, we focused on inhibition of testosterone 5α-reductase, which is well recognized as one of the most effective strategies for the treatment of androgenic alopecia. RO-ext showed inhibitory activity of 82.4% and 94.6% at 200 and 500 µg/mL, respectively. As an active constituent of 5α-reductase inhibition, 12-methoxycarnosic acid was identified with activity-guided fractionation. In addition, the extract of R. officinalis and 12-methoxycarnosic acid inhibited androgen-dependent proliferation of LNCaP cells as 64.5% and 66.7% at 5 µg/mL and 5 μM, respectively. These results suggest that they inhibit the binding of dihydrotestosterone to androgen receptors. Consequently, RO-ext is a promising crude drug for hair growth.

  17. Meiosis of anther culture regenerants in asparagus (Asparagus officinalis L.

    Directory of Open Access Journals (Sweden)

    Leonardo Galli

    1998-03-01

    Full Text Available Pollen mother cells obtained from regenerated plants of asparagus (Asparagus officinalis L., in a population composed exclusively of male plants, through the process of anther culture from the hybrid G27 X 22-8, were analyzed during meiosis. It was observed that, during theprocess of anther culture by organogenesis, the pollen mother cells of the regenerants had great genomic instability, as evidenced by disturbances in all the meiotic phases of the first and second division. Furthermore, structural chromosomal abnormalities, in addition to aneuploidy and polyploidy, were observed.Foi analisada a meiose em células mãe de pólen de plantas de aspargo (Asparagus officinalis L. de uma população composta exclusivamente de plantas masculinas, obtidas através do processo de cultura de anteras do híbrido G27 X 22-8. Foi observado que, durante o processo de cultura de anteras, via calogênese, as células mãe de pólen dos regenerantes apresentaram grande instabilidade genômica, evidenciada por irregularidades nas fases de diacinese, assim como de metáfase, anáfase, telófase da primeira e segunda divisão meiótica. Além disto, o processo originou anormalidades cromossômicas estruturais em adição às aneuploidias e poliploidias.

  18. Ecophysiological and antioxidant traits of Salvia officinalis under ozone stress.

    Science.gov (United States)

    Pellegrini, Elisa; Francini, Alessandra; Lorenzini, Giacomo; Nali, Cristina

    2015-09-01

    Ecophysiological and antioxidant traits were evaluated in sage (Salvia officinalis) plants exposed to 120 ppb of ozone for 90 consecutive days (5 h day(-1)). At the end of fumigation, plants showed slight leaf yellowing that could be considered the first visual symptom of leaf senescence. Ozone-stressed leaves showed (1) reduced photosynthetic activity (-70 % at the end of exposure), (2) chlorophyll loss (-59 and -56 % of chlorophyll a and b concentrations, starting from 30 days from the beginning of exposure), and (3) cellular water deficit (-12 % of the relative water content at the end of the fumigation). These phenomena are indicative of oxidative stress in the chloroplasts (as confirmed by the strong degradation of β-carotene) despite the photoprotection conferred by xanthophyll cycle [as demonstrated by the significant rise of de-epoxidation index, reaching the maximum value at the end of the treatment (+69 %)], antioxidant compounds [as confirmed by the increase of phenols (in particular caffeic acid and rosmarinic acid)], and water-soluble carbohydrates (especially monosaccharides). By means of combined ecophysiological and biochemical approaches, this study demonstrates that S. officinalis is able to activate an adaptive survival mechanism allowing the plant to complete its life cycle even under oxidative stressful conditions.

  19. The Lin28/Let-7 system in early human embryonic tissue and ectopic pregnancy.

    Science.gov (United States)

    Lozoya, Teresa; Domínguez, Francisco; Romero-Ruiz, Antonio; Steffani, Liliana; Martínez, Sebastián; Monterde, Mercedes; Ferri, Blanca; Núñez, Maria Jose; AinhoaRomero-Espinós; Zamora, Omar; Gurrea, Marta; Sangiao-Alvarellos, Susana; Vega, Olivia; Simón, Carlos; Pellicer, Antonio; Tena-Sempere, Manuel

    2014-01-01

    Our objective was to determine the expression of the elements of the Lin28/Let-7 system, and related microRNAs (miRNAs), in early stages of human placentation and ectopic pregnancy, as a means to assess the potential role of this molecular hub in the pathogenesis of ectopic gestation. Seventeen patients suffering from tubal ectopic pregnancy (cases) and forty-three women with normal on-going gestation that desired voluntary termination of pregnancy (VTOP; controls) were recruited for the study. Embryonic tissues were subjected to RNA extraction and quantitative PCR analyses for LIN28B, Let-7a, miR-132, miR-145 and mir-323-3p were performed. Our results demonstrate that the expression of LIN28B mRNA was barely detectable in embryonic tissue from early stages of gestation and sharply increased thereafter to plateau between gestational weeks 7-9. In contrast, expression levels of Let-7, mir-132 and mir-145 were high in embryonic tissue from early gestations (≤ 6-weeks) and abruptly declined thereafter, especially for Let-7. Opposite trends were detected for mir-323-3p. Embryonic expression of LIN28B mRNA was higher in early stages (≤ 6-weeks) of ectopic pregnancy than in normal gestation. In contrast, Let-7a expression was significantly lower in early ectopic pregnancies, while miR-132 and miR-145 levels were not altered. Expression of mir-323-3p was also suppressed in ectopic embryonic tissue. We are the first to document reciprocal changes in the expression profiles of the gene encoding the RNA-binding protein, LIN28B, and the related miRNAs, Let-7a, mir-132 and mir-145, in early stages of human placentation. This finding suggests the potential involvement of LIN28B/Let-7 (de)regulated pathways in the pathophysiology of ectopic pregnancy in humans.

  20. Synergistic antibacterial activity of Salvia officinalis and Cichorium intybus extracts and antibiotics.

    Science.gov (United States)

    Stefanović, Olgica D; Stanojević, Dragana D; Comić, Ljiljana R

    2012-01-01

    Synergistic activity of Salvia officinalis and Cichorium intybus extracts and commonly used antibiotics, amoxicillin and chloramphenicol, were evaluated. Interactions between plant extracts and antibiotics were tested by checkerboard method and interpreted as FIC index. Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and clinical isolates Staphylococcus aureus, Bacillus subtilis, Enterobacter cloacae, Klebsiella pneumoniae, Escherichia coli and Proteus mirabilis were used. Salvia officinalis showed better synergistic capacity than Cichorium intybus. Synergistic interactions were observed between amoxicillin and acetone or ethyl acetate extract of Salvia officinalis and between chloramphenicol and ethyl acetate extract of Salvia officinalis. In the presence of sub-inhibitory concentration (1/4 MIC to 1/32 MIC) of sage extracts, the MIC values of antibiotics were decreased by 2- to 10-fold. Synergism was observed against all test bacteria, except Escherichia coli. The combinations of acetone and ethyl acetate extract from Cichorium intybus and antibiotics resulted in additive and indifferent effects against tested bacteria.

  1. Phytochemical profile of Rosmarinus officinalis and Salvia officinalis extracts and correlation to their antioxidant and anti-proliferative activity.

    Science.gov (United States)

    Kontogianni, Vassiliki G; Tomic, Goran; Nikolic, Ivana; Nerantzaki, Alexandra A; Sayyad, Nisar; Stosic-Grujicic, Stanislava; Stojanovic, Ivana; Gerothanassis, Ioannis P; Tzakos, Andreas G

    2013-01-01

    The goal of this study was to monitor the anti-proliferative activity of Rosmarinus officinalis and Salvia officinalis extracts against cancer cells and to correlate this activity with their phytochemical profiles using liquid chromatography/diode array detection/electrospray ion trap tandem mass spectrometry (LC/DAD/ESI-MS(n)). For the quantitative estimation of triterpenic acids in the crude extracts an NMR based methodology was used and compared with the HPLC measurements, both applied for the first time, for the case of betulinic acid. Both extracts exerted cytotoxic activity through dose-dependent impairment of viability and mitochondrial activity of rat insulinoma m5F (RINm5F) cells. Decrease of RINm5F viability was mediated by nitric oxide (NO)-induced apoptosis. Importantly, these extracts potentiated NO and TNF-α release from macrophages therefore enhancing their cytocidal action. The rosemary extract developed more pronounced antioxidant, cytotoxic and immunomodifying activities, probably due to the presence of betulinic acid and a higher concentration of carnosic acid in its phytochemical profile.

  2. Symmetry group theorem to the Lin-Tsien equation and conservation laws relating to the symmetry of the equation?

    OpenAIRE

    Xi-Zhong, Liu

    2012-01-01

    In this paper, We derive the symmetry group theorem to the Lin-Tsien equation by using the modified CK's direct method, from which we obtain the corresponding symmetry group. More importantly, conservation laws corresponding to the Kac-Moody-Virasoro symmetry algebra of Lin-Tsien equation is obtained up to second order group invariants.

  3. Kinetic and sequence-structure-function analysis of known LinA variants with different hexachlorocyclohexane isomers.

    Directory of Open Access Journals (Sweden)

    Pooja Sharma

    Full Text Available BACKGROUND: Here we report specific activities of all seven naturally occurring LinA variants towards three different isomers, α, γ and δ, of a priority persistent pollutant, hexachlorocyclohexane (HCH. Sequence-structure-function differences contributing to the differences in their stereospecificity for α-, γ-, and δ-HCH and enantiospecificity for (+- and (--α -HCH are also discussed. METHODOLOGY/PRINCIPAL FINDINGS: Enzyme kinetic studies were performed with purified LinA variants. Models of LinA2(B90A A110T, A111C, A110T/A111C and LinA1(B90A were constructed using the FoldX computer algorithm. Turnover rates (min(-1 showed that the LinAs exhibited differential substrate affinity amongst the four HCH isomers tested. α-HCH was found to be the most preferred substrate by all LinA's, followed by the γ and then δ isomer. CONCLUSIONS/SIGNIFICANCE: The kinetic observations suggest that LinA-γ1-7 is the best variant for developing an enzyme-based bioremediation technology for HCH. The majority of the sequence variation in the various linA genes that have been isolated is not neutral, but alters the enantio- and stereoselectivity of the encoded proteins.

  4. Effects of Rosmarinus officinalis essential oil on germ tube formation by Candida albicans isolated from denture wearers

    OpenAIRE

    Lurdete Maria Rocha Gauch; Fabíola Silveira-Gomes; Renata Antunes Esteves; Simone Soares Pedrosa; Ely Simone Cajueiro Gurgel; Alberto Cardoso Arruda; Silvia Helena Marques-da-Silva

    2014-01-01

    Introduction The aim of this study was to investigate the effects of Rosmarinus officinalis essential oil on germ tube formation by Candida albicans isolated from denture wearers. Methods Ten C. albicans isolates recovered from denture wearers were tested using 10% fetal bovine serum with or without 4% R. officinalis essential oil. Results The essential oil from R. officinalis completely inhibited germ tube formation in the investigated C. albicans isolates. Conclusions The results demo...

  5. Analgesic Effect of Indian Gooseberry (Emblica officinalis Fruit Extracts on Postoperative and Neuropathic Pain in Rats

    Directory of Open Access Journals (Sweden)

    Dong Wook Lim

    2016-11-01

    Full Text Available Indian gooseberry (Emblica officinalis fruit, also known as “Amla” is one of the oldest edible fruits known in India. It has also traditionally been used to treat inflammation, and as an analgesic to treat wounds. However, experimental evidence for the analgesic effects of E. officinalis has been lacking. The present study investigated whether E. officinalis extracts exhibit analgesic effects in the plantar incision (PI and spared nerve injury (SNI pain-model rats. We evaluated the mechanical withdrawal threshold (MWT using von Frey filaments, and pain-related behavior was determined after surgery based on ultrasonic vocalization (USV. The group treated with E. officinalis extracts at 300 mg/kg had significantly increased MWT values at 6 h and 24 h after the PI, and had a significantly reduced number of 22–27-kHz USVs at 6 h and 24 h after PI. Moreover, after 15 days of continuous treatment with E. officinalis extracts, the treated group showed significantly alleviated SNI-induced hypersensitivity and reduced pro-inflammatory cytokine levels. Thus, E. officinalis extracts have potential analgesic effects in both postoperative and neuropathic pain models in vivo.

  6. Analgesic Effect of Indian Gooseberry (Emblica officinalis Fruit) Extracts on Postoperative and Neuropathic Pain in Rats

    Science.gov (United States)

    Lim, Dong Wook; Kim, Jae Goo; Kim, Yun Tai

    2016-01-01

    Indian gooseberry (Emblica officinalis fruit), also known as “Amla” is one of the oldest edible fruits known in India. It has also traditionally been used to treat inflammation, and as an analgesic to treat wounds. However, experimental evidence for the analgesic effects of E. officinalis has been lacking. The present study investigated whether E. officinalis extracts exhibit analgesic effects in the plantar incision (PI) and spared nerve injury (SNI) pain-model rats. We evaluated the mechanical withdrawal threshold (MWT) using von Frey filaments, and pain-related behavior was determined after surgery based on ultrasonic vocalization (USV). The group treated with E. officinalis extracts at 300 mg/kg had significantly increased MWT values at 6 h and 24 h after the PI, and had a significantly reduced number of 22–27-kHz USVs at 6 h and 24 h after PI. Moreover, after 15 days of continuous treatment with E. officinalis extracts, the treated group showed significantly alleviated SNI-induced hypersensitivity and reduced pro-inflammatory cytokine levels. Thus, E. officinalis extracts have potential analgesic effects in both postoperative and neuropathic pain models in vivo. PMID:27898027

  7. Rosmarinus officinalis polyphenols produce anti-depressant like effect through monoaminergic and cholinergic functions modulation.

    Science.gov (United States)

    Sasaki, Kazunori; El Omri, Abdelfatteh; Kondo, Shinji; Han, Junkyu; Isoda, Hiroko

    2013-02-01

    Rosmarinus officinalis (R. officinalis), a culinary aromatic and medicinal plant, is very rich in polyphenols and flavonoids with high antioxidant properties. This plant was reported to exert multiple benefits for neuronal system and alleviate mood disorder. In our previous study, we demonstrated that R. officinalis and its active compounds, luteolin (Lut), carnosic acid (CA), and rosmarinic acid (RA), exhibited neurotrophic effects and improved cholinergic functions in PC12 cells in correlation with mitogen-activated protein kinase (MAPK), ERK1/2 signaling pathway. The current study was conducted to evaluate and understand the anti-depressant effect of R. officinalis using tail suspension test (TST) in ICR mice and PC12 cells as in vitro neuronal model. Proteomics analysis of PC12 cells treated with R. officinalis polyphenols (ROP) Lut, CA, and RA revealed a significant upregulation of tyrosine hydroxylase (TH) and pyruvate carboxylase (PC) two major genes involved in dopaminergic, serotonergic and GABAergic pathway regulations. Moreover, ROP were demonstrated to protect neuronal cells against corticosterone-induced toxicity. These results were concordant with decreasing immobility time in TST and regulation of several neurotransmitters (dopamine, norepinephrine, serotonin and acetylcholine) and gene expression in mice brain like TH, PC and MAPK phosphatase (MKP-1). To the best of our knowledge this is the first evidence to contribute to the understanding of molecular mechanism behind the anti-depressant effect of R. officinalis and its major active compounds.

  8. Preliminary phytochemical, acute oral toxicity and antihepatotoxic study of roots of Paeonia officinalis Linn

    Institute of Scientific and Technical Information of China (English)

    Feroz Ahmad; Nahida Tabassum

    2013-01-01

    Objective: To carry out a preliminary phytochemical, acute oral toxicity and antihepatotoxic study of the roots of Paeonia officinalis (P. officinalis) L. Methods: Preliminary phytochemical investigation was done as per standard procedures. Acute oral toxicity study was conducted as per OECD 425 guidelines. The antihepatotoxic activity of aqueous extract of root of P. officinalis was evaluated against carbon tetrachloride (CCl4) induced hepatic damage in rats. Aqueous extract of P. officinalis at the dose levels of 100 and 200 mg/kg body weight was administered daily for 14 d in experimental animals. Liver injury was induced chemically, by CCl4 administration (1 mL/kg i.p.). The hepatoprotective activity was assessed using various biochemical parameters like aspartate aminotransferase (AST), alanine aminotransferase (ALT), serum alkaline phosphatase (SALP), total bilirubin and total protein (TP) along with histopathological studies. Result: Phytochemical screening revealed that the roots ofP. officinalis contain alkaloids, tannins, saponins, glycosides, carbohydrates, flavonoids, terpenes, steroids and proteins. The aqueous extract did not cause any mortality up to 2000 mg/kg. In rats that had received the root extract at the dose of 100 and 200 mg/kg, the substantially elevated AST, ALT, SALP, total bilirubin levels were significantly lowered, respectively, in a dose dependent manner, along with CCl4 while TP levels were elevated in these groups. Histopathology revealed regeneration of the livers in extract treated groups while Silymarin treated rats were almost normal. Conclusions: The aqueous extract of P. officinalis is safe and possesses antihepatotoxic potential.

  9. Effects of Rosmarinus officinalis essential oil on germ tube formation by Candida albicans isolated from denture wearers

    Directory of Open Access Journals (Sweden)

    Lurdete Maria Rocha Gauch

    2014-06-01

    Full Text Available Introduction The aim of this study was to investigate the effects of Rosmarinus officinalis essential oil on germ tube formation by Candida albicans isolated from denture wearers. Methods Ten C. albicans isolates recovered from denture wearers were tested using 10% fetal bovine serum with or without 4% R. officinalis essential oil. Results The essential oil from R. officinalis completely inhibited germ tube formation in the investigated C. albicans isolates. Conclusions The results demonstrate that the essential oil of R. officinalis modulates C. albicans pathogenicity through its primary virulence factor (i.e., germ tube formation was suppressed.

  10. Cryptanalysis of Lin et al.'s Efficient Block-Cipher-Based Hash Function

    NARCIS (Netherlands)

    Liu, Bozhong; Gong, Zheng; Chen, Xiaohong; Qiu, Weidong; Zheng, Dong

    2010-01-01

    Hash functions are widely used in authentication. In this paper, the security of Lin et al.'s efficient block-cipher-based hash function is reviewed. By using Joux's multicollisions and Kelsey et al.'s expandable message techniques, we find the scheme is vulnerable to collision, preimage and second

  11. The Value of SCMC in SLA: Comments on Lin, Huang & Liou (2013)

    Science.gov (United States)

    Taylor, Alan M.

    2014-01-01

    Meta-analytic methods are often used to determine the effectiveness of certain treatments across studies. However, we are often unaware of how a meta-analysis can provide value to researchers and practitioners. This paper offers a brief commentary on a meta-analysis conducted by Lin, Huang and Liou (2013) in LLT, providing further statistical…

  12. New explicit and exact solutions of the Benney-Kawahara-Lin equation

    Institute of Scientific and Technical Information of China (English)

    Xie Yuan-Xi

    2009-01-01

    In this paper, we present a combination method of constructing the explicit and exact solutions of nonlinear partial differential equations. And as an illustrative example, we apply the method to the Benney-Kawahara-Lin equation and derive its many explicit and exact solutions which are all new solutions.

  13. Single-tube linear DNA amplification (LinDA) for robust ChIP-seq

    NARCIS (Netherlands)

    Shankaranarayanan, P.; Mendoza-Parra, M.A.; Walia, M.; Wang, L.; Li, N.; Trindade, L.M.; Gronemeyer, H.

    2011-01-01

    Genome-wide profiling of transcription factors based on massive parallel sequencing of immunoprecipitated chromatin (ChIP-seq) requires nanogram amounts of DNA. Here we describe a high-fidelity, single-tube linear DNA amplification method (LinDA) for ChIP-seq and reChIP-seq with picogram DNA amounts

  14. linäituse stendid = Outdoor exhibition stands / Gert Sarv, Galina Burnakova

    Index Scriptorium Estoniae

    Sarv, Gert, 1971-

    2013-01-01

    Eesti Sisearhitektide Liidu 2012. aasta parima eseme preemia pälvinud välinäituse stendidest Vanasadama Admiraliteedi basseini kaldapealsel. Autorid: Gert Sarv, Galina Burnakova, Loreida Hein. Sisearhitekti ja ESLi aastapreemiate žürii liikme Tea Tammelaane arvamus

  15. Anticonvulsant, Anxiolytic, and Sedative Activities of Verbena officinalis

    Science.gov (United States)

    Khan, Abdul Waheed; Khan, Arif-ullah; Ahmed, Touqeer

    2016-01-01

    We describe different neuropharmacological effects of Verbena officinalis crude extract (Vo.Cr). Pentylenetetrazole (PTZ)-induced seizures, elevated plus maze, light–dark box (LDB), open field and thiopental-induced sleeping test models were employed to evaluate Vo.Cr actions in mice. Vo.Cr dose-dependently (100–500 mg/Kg) delayed onset time of myoclonic jerks and tonic-clonic seizures, while decreased duration of tonic-clonic seizures (P diazepam. In open field test, Vo.Cr decreased number of ambulations and rearings frequencies, while increased the number of central squares crossings. In thiopental-induced sleeping test, Vo.Cr (50–300 mg/Kg) decreased onset time of sleep, while increased the duration of sleep (P anxiety, and insomnia. PMID:28066246

  16. Rosmarinus officinalis leaves as a natural source of bioactive compounds.

    Science.gov (United States)

    Borrás-Linares, Isabel; Stojanović, Zorica; Quirantes-Piné, Rosa; Arráez-Román, David; Švarc-Gajić, Jaroslava; Fernández-Gutiérrez, Alberto; Segura-Carretero, Antonio

    2014-11-10

    In an extensive search for bioactive compounds from plant sources, the composition of different extracts of rosemary leaves collected from different geographical zones of Serbia was studied. The qualitative and quantitative characterization of 20 rosemary (Rosmarinus officinalis) samples, obtained by microwave-assisted extraction (MAE), was determined by high performance liquid chromatography coupled to electrospray quadrupole-time of flight mass spectrometry (HPLC-ESI-QTOF-MS). The high mass accuracy and true isotopic pattern in both MS and MS/MS spectra provided by the QTOF-MS analyzer enabled the characterization of a wide range of phenolic compounds in the extracts, including flavonoids, phenolic diterpenes and abietan-type triterpenoids, among others. According to the data compiled, rosemary samples from Sokobanja presented the highest levels in flavonoids and other compounds such as carnosol, rosmaridiphenol, rosmadial, rosmarinic acid, and carnosic acid. On the other hand, higher contents in triterpenes were found in the extracts of rosemary from Gložan (Vojvodina).

  17. New terpenoid glycosides obtained from Rosmarinus officinalis L. aerial parts.

    Science.gov (United States)

    Zhang, Yi; Adelakun, Tiwalade Adegoke; Qu, Lu; Li, Xiaoxia; Li, Jian; Han, Lifeng; Wang, Tao

    2014-12-01

    Five new terpenoid glycosides, named as officinoterpenosides A₁ (1), A₂ (2), B (3), C (4), and D (5), together with 11 known ones, (1S,4S,5S)-5-exo-hydrocamphor 5-O-β-D-glucopyranoside (6), isorosmanol (7), rosmanol (8), 7-methoxyrosmanol (9), epirosmanol (10), ursolic acid (11), micromeric acid (12), oleanolic acid (13), niga-ichigoside F₁ (14), glucosyl tormentate (15), and asteryunnanoside B (16), were obtained from the aerial parts of Rosmarinus officinalis L. Their structures were elucidated by chemical and spectroscopic methods (UV, IR, HRESI-TOF-MS, 1D and 2D NMR). Among the new ones, 1 and 2, 3 and 4 are diterpenoid and triterpenoid glycosides, respectively; and 5 is a normonoterpenoid. For the known ones, 6 was isolated from the Rosmarinus genus first, and 15, 16 were obtained from this species for the first time.

  18. Identification of residues of the Caenorhabditis elegans LIN-1 ETS domain that are necessary for DNA binding and regulation of vulval cell fates.

    Science.gov (United States)

    Miley, Ginger R; Fantz, Douglas; Glossip, Danielle; Lu, Xiaowei; Saito, R Mako; Palmer, Robert E; Inoue, Takao; Van Den Heuvel, Sander; Sternberg, Paul W; Kornfeld, Kerry

    2004-08-01

    LIN-1 is an ETS domain protein. A receptor tyrosine kinase/Ras/mitogen-activated protein kinase signaling pathway regulates LIN-1 in the P6.p cell to induce the primary vulval cell fate during Caenorhabditis elegans development. We identified 23 lin-1 loss-of-function mutations by conducting several genetic screens. We characterized the molecular lesions in these lin-1 alleles and in several previously identified lin-1 alleles. Nine missense mutations and 10 nonsense mutations were identified. All of these lin-1 missense mutations affect highly conserved residues in the ETS domain. These missense mutations can be arranged in an allelic series; the strongest mutations eliminate most or all lin-1 functions, and the weakest mutation partially reduces lin-1 function. An electrophoretic mobility shift assay was used to demonstrate that purified LIN-1 protein has sequence-specific DNA-binding activity that required the core sequence GGAA. LIN-1 mutant proteins containing the missense substitutions had dramatically reduced DNA binding. These experiments identify eight highly conserved residues of the ETS domain that are necessary for DNA binding. The identification of multiple mutations that reduce the function of lin-1 as an inhibitor of the primary vulval cell fate and also reduce DNA binding suggest that DNA binding is essential for LIN-1 function in an animal.

  19. Simultaneous determination of bifonazole and tinctures of calendula flower in pharmaceutical creams by reversed-phase liquid chromatography.

    Science.gov (United States)

    Ferreyra, Carola F; Ortiz, Cristina S

    2005-01-01

    The aim of this research was to develop and validate a sensitive, rapid, easy, and precise reversed-phase liquid chromatography (LC) method for stability studies of bifonazole (I) formulated with tinctures of calendula flower (II). The method was especially developed for the analysis and quantitative determination of I and II in pure and combined forms in cream pharmaceutical formulations without using gradient elution and at room temperature. The influence on the stability of compound I of temperature, artificial radiation, and drug II used for the new pharmaceutical design was evaluated. The LC separation was carried out using a Supelcosil LC-18 column (25 cm x 4.6 mm id, 5 microm particle size); the mobile phase was composed of methanol-0.1 M ammonium acetate buffer (85 + 15, v/v) pumped isocratically at a flow rate of 1 mL/min; and ultraviolet detection was at 254 nm. The analysis time was less than 10 min. Calibration graphs were found to be linear in the 0.125-0.375 mg/mL (rI = 0.9991) and 0.639-1.916 mg/mL (rII = 0.9995) ranges for I and II, respectively. The linearity, precision, recovery, and limits of detection and quantification were satisfactory for I and II. The results obtained suggested that the developed LC method is selective and specific for the analysis of I and II in pharmaceutical products, and that it can be applied to stability studies.

  20. Chemical variability, antioxidant and antifungal activities of essential oils and hydrosol extract of Calendula arvensis L. from western Algeria.

    Science.gov (United States)

    Belabbes, Rania; Dib, Mohammed El Amine; Djabou, Nassim; Ilias, Faiza; Tabti, Boufeldja; Costa, Jean; Muselli, Alain

    2017-01-21

    The chemical composition of the essential oils and hydrosol extract from aerial parts of Calendula arvensis L. was investigated using GC-FID and GC/MS. Intra-species variations of the chemical compositions of essential oils from 18 Algerian sample locations were investigated using statistical analysis. Chemical analysis allowed the identification of fifty-three compounds amounting to 92.3-98.5% with yields varied of 0.09-0.36% and the main compounds were zingiberenol 1 (8.7-29.8%), eremoligenol (4.2-12.5%), β-curcumene (2.1-12.5%), zingiberenol 2 (4.6-19.8%) and (E,Z)-farnesol (3.5-23.4%). The study of the chemical variability of essential oils allowed the discrimination of two main clusters confirming that there is a relation between the essential oil compositions and the harvest locations. Different concentrations of essential oil and hydrosol extract were prepared and their antioxidant activity were assessed using three methods (DPPH, FRAP and β-carotene). The results showed that hydrosol extract presented an interesting antioxidant activity. The in vitro antifungal activity of hydrosol extract produced the best antifungal inhibition against Penicillium expansum and Aspergillus niger, while, essential oil was inhibitory at relatively higher concentrations. Results showed that the treatments of pear fruits with essential oil and hydrosol extract presented a very interesting protective activity on disease severity of pears caused by P. expansum. This article is protected by copyright. All rights reserved.

  1. Intron-specific patterns of divergence of lin-11 regulatory function in the C. elegans nervous system.

    Science.gov (United States)

    Amon, Siavash; Gupta, Bhagwati P

    2017-04-01

    The diversity of neurons in the nervous system is specified by many genes, including those that encode transcription factors (TFs) and play crucial roles in coordinating gene transcription. To understand how the spatiotemporal expression of TF genes is regulated to generate neuronal diversity, we used one member of the LIM-Hox family, lin-11, as a model that is necessary for the differentiation of amphid neurons in the nematode C. elegans and a related species C. briggsae. We characterized transcriptional regulation of lin-11 and uncovered regulatory roles of two of the largest introns, intron 3 and intron 7. These introns promote lin-11 expression in non-overlapping sets of neurons. Phenotypic rescue experiments in C. elegans revealed that intron 3 is capable of restoring lin-11 function based on gene expression patterns and behavioral assays. Interestingly, intron 3-driven reporter expression showed differences in neuronal cell types between C. briggsae and C. elegans, indicating evolutionary changes in lin-11 regulation between the two species. Reciprocal transformation experiments provided further evidence consistent with functional changes in both cis and trans regulation of lin-11. To further investigate transcriptional regulation of lin-11, we dissected the intronic regions in C. elegans and identified cell-specific enhancers. These enhancers possess multiple sequence blocks that are conserved among Caenorhabditis species and possess TF binding sites. We tested the role of a subset of predicted TFs and discovered that while three of them (SKN-1, CEH-6, and CRH-1) act via the intron 3 enhancer to negatively regulate lin-11 expression in neurons, another TF (CES-1) acts positively via the intron 7 enhancer. Overall, our findings demonstrate that neuronal expression of lin-11 involves multiple TF regulators and regulatory modules some of which have diverged in Caenorhabditis nematodes.

  2. Using NMR to Identify Structural Features of Lin28-Regulated miRNAs and mRNAs and as a Tool for Comparing Differences in Cellular Metabolism

    OpenAIRE

    O'Day, Elizabeth Mary

    2013-01-01

    Part 1 of this thesis seeks to identify shared structural features of Lin28-regulated miRNAs and mRNAs. Lin28 is an evolutionarily conserved, RNA binding protein, highly expressed in stem cells and poorly differentiated cancers, that inhibits differentiation and helps maintain stem cell properties. Lin28 binds to both the loops of let-7 precursors to block let-7 biogenesis and to Lin28 responsive elements (LREs) in mRNAs either to enhance or inhibit translation. Lin28 RNA binding properties a...

  3. Chemical composition of Rosmarinus officinalis essential oil and antioxidant action against gastric damage induced by absolute ethanol in the rat

    Institute of Scientific and Technical Information of China (English)

    Christiane Takayama; Felipe Meira de-Faria; Ana Cristina Alves de Almeida; Ricardo José Dunder; Luis Paulo Manzo; Eduardo Augusto Rabelo Socca; Leonia Maria Batista; Marcos José Salvador; Alba Regina Monteiro Souza-Brito; Anderson Luiz-Ferreira

    2016-01-01

    Objective: To evaluate the antioxidant activity of the essential oil obtained from Rosmarinus officinalis(R. officinalis) in ethanol-induced gastric ulcer model in vivo.Methods: The antioxidant properties of the essential oil obtained from R. officinalis were evaluated against gastric injury induced by absolute ethanol. Gastric tissues were prepared to enzymatic assays. The levels of glutathione, lipid peroxides, and the activities of glutathione peroxidase, superoxide dismutase and myeloperoxidase were measured.Results: Ethanol produced severe hemorrhagic lesions in the stomach with ulcerative lesion of(140.2 ± 37.2) mm2. In animals pretreated with essential oil of R. officinalis(50 mg/kg, p.o.), a significant inhibition of mucosal injury of(21.2 ± 7.1) mm2(84%inhibition) was observed. The essential oil of R. officinalis protected the gastric mucosa probably by modulating the activities of the enzymes(superoxide dismutase and glutathione peroxidase) and increasing or maintaining the levels of glutathione. In addition,lipid peroxides levels were reduced. The essential oil of R. officinalis was analyzed by gas chromatography–mass spectrometer and the main constituents were cineole(28.5%),camphor(27.7%) and alpha-pinene(21.3%).Conclusions: We suggest that the monoterpenes present in the essential oil obtained from R. officinalis may be among the active principles responsible for the antioxidant activity shown by essential oil of R. officinalis.

  4. Chemical composition of Rosmarinus officinalis essential oil and antioxidant action against gastric damage induced by absolute ethanol in the rat

    Institute of Scientific and Technical Information of China (English)

    Christiane Takayama; Anderson Luiz-Ferreira; Felipe Meira de-Faria; Ana Cristina Alves de Almeida; Ricardo Jose Dunder; Luis Paulo Manzo; Eduardo Augusto Rabelo Socca; Leonia Maria Batista; Marcos Jos e Salvador

    2016-01-01

    Objective: To evaluate the antioxidant activity of the essential oil obtained from Ros-marinus officinalis (R. officinalis) in ethanol-induced gastric ulcer model in vivo. Methods: The antioxidant properties of the essential oil obtained from R. officinalis were evaluated against gastric injury induced by absolute ethanol. Gastric tissues were pre-pared to enzymatic assays. The levels of glutathione, lipid peroxides, and the activities of glutathione peroxidase, superoxide dismutase and myeloperoxidase were measured. Results: Ethanol produced severe hemorrhagic lesions in the stomach with ulcerative lesion of (140.2 ± 37.2) mm2. In animals pretreated with essential oil of R. officinalis (50 mg/kg, p.o.), a significant inhibition of mucosal injury of (21.2 ± 7.1) mm2 (84%inhibition) was observed. The essential oil of R. officinalis protected the gastric mucosa probably by modulating the activities of the enzymes (superoxide dismutase and gluta-thione peroxidase) and increasing or maintaining the levels of glutathione. In addition, lipid peroxides levels were reduced. The essential oil of R. officinalis was analyzed by gas chromatography-mass spectrometer and the main constituents were cineole (28.5%), camphor (27.7%) and alpha-pinene (21.3%). Conclusions: We suggest that the monoterpenes present in the essential oil obtained from R. officinalis may be among the active principles responsible for the antioxidant activity shown by essential oil of R. officinalis.

  5. Brachycorynella asparagi (Mordv.) Induced—Oxidative Stress and Antioxidative Defenses of Asparagus officinalis L.

    Science.gov (United States)

    Borowiak-Sobkowiak, Beata; Woźniak, Agnieszka; Bednarski, Waldemar; Formela, Magda; Samardakiewicz, Sławomir; Morkunas, Iwona

    2016-01-01

    The aim of this study was to investigate whether and to what extent oxidative stress is induced in leaves of one- and two-month-old plants of Asparagus officinalis L. cv. Argenteuil infested by Brachycorynella asparagi (Mordvilko) at a varied population size. The pest B. asparagi has been described as the most damaging species feeding on asparagus. Analyses using electron paramagnetic resonance (EPR) demonstrated generally higher concentrations of semiquinone radicals with g-values of 2.0045 ± 0.0005 and 2.0026 ± 0.0005 in Asparagus officinalis (A. officinalis) leaves after Brachycorynella asparagi (B. asparagi) infestation than in the control. Observations of leaves under a confocal microscope showed a post-infestation enhanced generation of the superoxide anion radical (O2•−) and hydrogen peroxide (H2O2) in comparison to the control. Strong fluctuations in Mn2+ ion levels detected by EPR spectroscopy versus time were detected in leaves infested by aphids, which may indicate the involvement of these ions in the control of O2•− production. An enhanced superoxide dismutase activity is an important element in leaf defense against oxidative stress. Visible symptoms were found in aphid-infested A. officinalis. Damage to leaves of one- and two-month-old A. officinalis plants by the aphid B. asparagi was dependent on the intensity, duration of infestation and plant age. PMID:27775613

  6. Variation of essential oil composition of Melissa officinalis L. leaves during different stages of plant growth

    Institute of Scientific and Technical Information of China (English)

    Keivan Saeb; Sara Gholamrezaee

    2012-01-01

    Objective: To determine the best time of harvest for Melissa officinalis (M. officinalis) L. to gain highest amounts essential oil.Methods: M. officinalis leaves were harvested in three different stages (before flowering stage, flowering stage and after of flowering stage) and were dried. The essential oils were isolated by hydro- distillation and analyzed by GC/MS.Results: It showed that most essential oils of plants were in before flowering stage. In before flowering stage 37 compounds were identified in leaves oil of M.officinalis. The major components before flowering stage were decadienal (29.38%), geraniol (25.3%), caryophyllene oxide (8.75%), geranyl acetate (5.41%). In the flowering stage 36 compounds were identified as the major components of plant essential oils: decadienal (28.04%), geraniol (24.97%), caryophyllene oxide (7.55%), caryophyllene E (4.65%) and 16 components in the after flowering stage of plant were identified as the major components carvacrol (37.62%), methyl citronellate (32.34%), geranyl acetate (5.82%), caryophyllene (5.50%).Conclusions: The essential oils yields vary considerably from month-to-month and is also influenced by the micro-environment (sun or shade) in which the plant is growing. We found that the essential oil content of M. officinalis L. of leaves is significantly affected by harvesting stages.

  7. Preliminary assessment of trace elements effects on essential oil production of Melissa officinalis L. (Lemon balm)

    Energy Technology Data Exchange (ETDEWEB)

    Sussa, Fabio V.; Duarte, Celina L.; Andrade, Luana S.; Silva, Paulo S.C., E-mail: pscsilva@ipen.br, E-mail: clduarte@ipen.br, E-mail: fabiosussa@uol.com.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Furlan, Marcos R., E-mail: furlanagro@gmail.com [Universidade de Taubate, SP (Brazil). Departamento de Ciencias Agrarias

    2013-07-01

    Melissa officinalis L., Lemon balm, (Lamiaceae) is an herb used as medicine, condiment and in the cosmetic and perfumery industry due to its essential oil. In this study a preliminary assessment of trace elements effects on essential oil production was performed in order to verify an improvement in its quality. The Melissa officinalis samples were harvested from three different soil sites localized in Ibirapuera Park, São Paulo, Brazil. Elemental concentration for the elements As, Ba, Br, Ce, Cl, Co, Cr, Cs, Eu, Fe, Hf, K, La, Lu, Mg, Mn, Na, Nd, Rb, Sb, Sc, Se, Sm, Ta, Tb, Ti, Th, U, V, Yb, Zn and Zr was determined by Instrumental Neutron Activation Analysis (INAA) in Melissa officinalis leaves and surrounding soil. The essential oil was extracted from its leaves by hydrodistillation process in Clevenger apparatus and analyzed by gas chromatography coupled to a mass spectrometer (GC-MS). Certified reference materials NIST SRM-1515 Apple Leaves, NIST SRM-1547 Peach Leaves and NIST SRM-1573a Tomato Leaves were analyzed for quality control. Our results showed Geranial and Neral were identified as the major compounds in the essential oil extracted from Melissa officinalis L. for all collected sites. However, the relative proportion of some chemical constituents was altered according to the site collected. The preliminary results showed that the production of essential oil by Melissa officinalis must be positively correlated with the concentrations of Rb, Zn and negatively correlated with Sc, Mn, La, K, Fe, and Cr. (author)

  8. Drought-tolerant rice germplasm developed from an Oryza officinalis transformation-competent artificial chromosome clone.

    Science.gov (United States)

    Liu, R; Zhang, H H; Chen, Z X; Shahid, M Q; Fu, X L; Liu, X D

    2015-10-29

    Oryza officinalis has proven to be a natural gene reservoir for the improvement of domesticated rice as it carries many desirable traits; however, the transfer of elite genes to cultivated rice by conventional hybridization has been a challenge for rice breeders. In this study, the conserved sequence of plant stress-related NAC transcription factors was selected as a probe to screen the O. officinalis genomic transformation-competent artificial chromosome library by Southern blot; 11 positive transformation-competent artificial chromosome clones were subsequently detected. By Agrobacterium-mediated transformation, an indica rice variety, Huajingxian 74 (HJX74), was transformed with a TAC clone harboring a NAC gene-positive genomic fragment from O. officinalis. Molecular analysis revealed that the O. officinalis genomic fragment was integrated into the genome of HJX74. The transgenic lines exhibited high tolerance to drought stress. Our results demonstrate that the introduction of stress-related transformation-competent artificial chromosome clones, coupled with a transgenic validation approach, is an effective method of transferring agronomically important genes from O. officinalis to cultivated rice.

  9. Antidermatophytic activity of hydroalcoholic extracts from Rosmarinus officinalis and Tetradenia riparia.

    Science.gov (United States)

    Endo, E H; Costa, G M; Nakamura, T U; Nakamura, C V; Dias Filho, B P

    2015-12-01

    Rosmarinus officinalis and Tetradenia riparia are used in folk medicine for the treatment of disease, including infectious diseases and skin disorders. The purpose of this study was to investigate the antifungal activity of hydroalcoholic extracts from R. officinalis and T. riparia against strains of Trichophyton rubrum, T. mentagrophytes and Microsporum gypseum. Hydroalcoholic extracts prepared with dried leaves from R. officinalis, Psidium guajava and T. riparia were assayed against dermatophyte species by the microdilution technique and by microscopy. R. officinalis and T. riparia were the most active against dermatophytes, as determined from the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC), and were investigated further. Fluorescence microscopy and scanning electron microscopy were used to investigate inhibition of hyphal growth by the two extracts, and showed a strong inhibition and an irregular growth pattern. Both extracts showed good action against dermatophytes, inhibiting fungal growth and causing alterations in their hyphae. Therefore, R. officinalis and T. riparia are potential sources of new compounds for the development of antifungal drugs.

  10. Brachycorynella asparagi (Mordv. Induced—Oxidative Stress and Antioxidative Defenses of Asparagus officinalis L.

    Directory of Open Access Journals (Sweden)

    Beata Borowiak-Sobkowiak

    2016-10-01

    Full Text Available The aim of this study was to investigate whether and to what extent oxidative stress is induced in leaves of one- and two-month-old plants of Asparagus officinalis L. cv. Argenteuil infested by Brachycorynella asparagi (Mordvilko at a varied population size. The pest B. asparagi has been described as the most damaging species feeding on asparagus. Analyses using electron paramagnetic resonance (EPR demonstrated generally higher concentrations of semiquinone radicals with g-values of 2.0045 ± 0.0005 and 2.0026 ± 0.0005 in Asparagus officinalis (A. officinalis leaves after Brachycorynella asparagi (B. asparagi infestation than in the control. Observations of leaves under a confocal microscope showed a post-infestation enhanced generation of the superoxide anion radical (O2•− and hydrogen peroxide (H2O2 in comparison to the control. Strong fluctuations in Mn2+ ion levels detected by EPR spectroscopy versus time were detected in leaves infested by aphids, which may indicate the involvement of these ions in the control of O2•− production. An enhanced superoxide dismutase activity is an important element in leaf defense against oxidative stress. Visible symptoms were found in aphid-infested A. officinalis. Damage to leaves of one- and two-month-old A. officinalis plants by the aphid B. asparagi was dependent on the intensity, duration of infestation and plant age.

  11. Cancer Prevention and Health Benefices of Traditionally Consumed Borago officinalis Plants.

    Science.gov (United States)

    Lozano-Baena, María-Dolores; Tasset, Inmaculada; Muñoz-Serrano, Andrés; Alonso-Moraga, Ángeles; de Haro-Bailón, Antonio

    2016-01-01

    Nowadays, healthy eating is increasing the demand of functional foods by societies as sources of bioactive products with healthy qualities. For this reason, we tested the safety of the consumption of Borago officinalis L. and its main phenolic components as well as the possibility of its use as a nutraceutical plant to help in cancer prevention. The in vivo Drosophila Somatic Mutation and Recombination Test (SMART) and in vitro HL-60 human cell systems were performed, as well-recognized methods for testing genotoxicity/cytotoxicity of bioactive compounds and plant products. B. officinalis and the tested compounds possess antigenotoxic activity. Moreover, B. officinalis wild type cultivar exerts the most antigenotoxic values. Cytotoxic effect was probed for both cultivars with IC50 values of 0.49 and 0.28 mg · mL(-1) for wild type and cultivated plants respectively, as well as their constituent rosmarinic acid and the assayed phenolic mixture (IC50 = 0.07 and 0.04 mM respectively). B. officinalis exerts DNA protection and anticarcinogenic effects as do its component rosmarinic acid and the mixture of the main phenolics presented in the plant. In conclusion, the results showed that B. officinalis may represent a high value plant for pleiotropic uses and support its consumption as a nutraceutical plant.

  12. Emblica officinalis improves glycemic status and oxidative stress in STZ induced type 2 diabetic model rats

    Institute of Scientific and Technical Information of China (English)

    Aneesa Ansari; Md Shahed Zaman Shahriar; Md Mehedi Hassan; Shukla Rani Das; Begum Rokeya; Md Anwarul Haque; Md Enamul Haque; Nirupam Biswas; Tama Sarkar

    2014-01-01

    Objective: To evaluate the antidiabetic and antioxidant potential of Emblica officinalis (E. officinalis) fruit on normal and type 2 diabetic rats. Methods: Type 2 diabetes was induced into the male Long-Evans rats. The rats were divided into nine groups including control groups receiving water, type 2 diabetic controls, type 2 diabetic rats treated with glibenclamide (T2GT) and type 2 diabetic rats treated with aqueous extract of fruit pulp of E. officinalis. They were fed orally for 8 weeks with a single feeding. Blood was collected by cutting the tail tip on 0 and 28 days and by decapitation on 56 day. Packed red blood cells and serum were used for evaluating different biochemical parameters. Results: Four weeks administration of aqueous extract of E. officinalis improved oral glucose tolerance in type 2 rats and after 8 weeks it caused significant (P<0.007) reduction in fasting serum glucose level compared to 0 day. Triglycerides decreased by 14% but there was no significant change in serum ALT, creatinine, cholesterol and insulin level in any group. Furthermore, reduced erythrocyte malondialdehyde level showed no significant change (P<0.07) but reduced glutathione content was found to be increased significantly (P<0.05).Conclusions:The aqueous extract of E. officinalis has a promising antidiabetic and antioxidant properties and may be considered for further clinical studies in drug development.

  13. Effect of hydroalcoholic extract of Lavandula officinalis on nicotine-induced convulsion in mice.

    Science.gov (United States)

    Arzi, A; Ahamehe, M; Sarahroodi, S

    2011-06-01

    Epilepsy an important CNS (central nervous system) problem that about 1% of world's population suffer of it. The aim of study was to evaluate of anticonvulsant effect of hydroalcoholic extract of Lavandula officinalis. In this study, anticonvulsant activity of the hydroalcoholic extract of Lavandula officinalis (L. officinalis) was studied against chemoconvulsant-induced seizures in male mice. Lavandula officinalis (100, 200, 400, 600 and 800 mg kg(-1)), diazepam (0.15 mg kg(-1)) and normal saline (10 mL kg(-1)) were injected intraperitoneally, respectively in different groups of mice, 30 min before nicotine (5 mg kg(-) i.p.). The onset time intensity and duration of convulsions and the percentage of death were recorded. Also the time-response (0, 15, 30, 45, 60 min before nicotine injection) for most effective dose of plant extract (600 mg kg(-1)) was investigated. The results showed that hydroalcoholic extract of Lavandula officinalis had anticonvulsant effect. The most effective dose of plant extract was 600 mg kg(-1). In time-response study for the most effective dose of extract (600 mg kg(-1)), the onset, duration and intensity of convulsion significantly (p < 0.05) increased, decreased and decreased, respectively for all tested times. The best response observed in 30, 45 and 60 min. The results showed significant anticonvulsant effect for hydroalcoholic extract of Lavandula.

  14. Effects of hydroalcoholic extract of Borago officinalis on naloxone-precipitated withdrawal syndrome in morphine-dependent mice

    Directory of Open Access Journals (Sweden)

    Zahra Rabiei

    2016-12-01

    Full Text Available The aim of the present study was to investigate the effect of hydroalcoholic extract of Borago officinalis on morphine withdrawal syndrome in mice. Morphine-dependent group received morphine for nine days and then received naloxone via intraperitoneal injection. Control group received saline for nine days. Post-treated group received B. officinalis extract intraperitoneally (100 mg/kg on the day 10 before naloxone injection. Co-treated group received B. officinalis extract intraperitoneally (100 mg/kg and morphine for nine days and then received naloxone. Extract-treated group received extract for nine days and then received naloxone. Naloxone injection significantly increased the frequency of jumping, blinking, ptosis, defecation, paw trembling, and two-legged standing in comparison to the control group. Co-treatment and post-treatment with B. officinalis extract significantly decreased the withdrawal symptoms. In conclusion, hydroalcoholic extract of B. officinalis significantly attenuated the symptoms of morphine withdrawal syndrome.

  15. GENII-LIN: a Multipurpose Health Physics Code Built on GENII-1.485

    Directory of Open Access Journals (Sweden)

    Marco Sumini

    2006-10-01

    Full Text Available The aim of the GENII-LIN project was to develop an open source multipurpose health physics code running on Linux platform, for calculating radiation dose and risk from radionuclides released to the environment. The general features of the GENII-LIN system include [1] capabilities for calculating radiation dose both for acute and chronic releases, with options for annual dose, committed dose and accumulated dose [2] capabilities for evaluating exposure pathways including direct exposure via water (swimming, boating, fishing, soil (buried and surface sources and air (semi-infinite cloud and finite cloud model, inhalation pathways and ingestion pathways. The release scenarios considered are: - acute release to air, from ground level or elevated sources, or to water; - chronic release to air, from ground level or elevated sources, or to water; - initial contamination of soil or surfaces. Keywords: radiation protection, Linux, health physics, risk analysis.

  16. Manipulation quantique de la lumière par un amplificateur non linéaire

    Science.gov (United States)

    Symul, T.; Bencheikh, K.; Levenson, J. A.

    2002-06-01

    Nous proposons un dispositif original, appelé Amplificateur Non Linéaire (ANL), permettant la génération et la manipulation d'états quantiques de la lumière. Ce dispositif permet une compression du bruit quantique de la lumière en dessous de la limite quantique standard plus efficace que celle obtenue par interactions non linéaires du second ordre ou du troisième ordre. Il permet également d'inverser les fluctuations quantiques en intensité de la lumière, et de produire des photons jumeaux ayant des corrélations quantiques plus élevées et plus robustes que ceux produits par un amplificateur paramétrique seul.

  17. Caractéristiques des huiles de lin et de chanvre

    Directory of Open Access Journals (Sweden)

    Morin Odile

    2015-11-01

    Full Text Available Les huiles de lin et de chanvre appartiennent à la famille des huiles riches en acides gras polyinsaturés contenant de l’acide alpha-linolénique (ALA. Le contexte actuel du déficit d’apport en ALA, précurseur indispensable des AGPI de la série des omégas 3 (ou n-3, comme en ses dérivés métaboliques supérieurs (EPA, DHA, fonde le regain d’intérêt pour les sources apportant le précurseur de ces acides gras essentiels. Les autorisations de commercialisation de l’huile de chanvre ou l’évolution réglementaire dans le cas de l’huile de lin en France, ont tenu compte de cet atout nutritionnel pourvu que les conditions de stabilité en conservation soient garanties.

  18. A Comparative Study on Different Fates between Jane Eyre and Lin Daiyu

    Institute of Scientific and Technical Information of China (English)

    林冬梅; 赵慧

    2014-01-01

    Jane Eyre and A Dream of Red Mansions can be regarded as the most famous two classics in the history of literature. In A Dream of Red Mansions, Cao Xueqin exhausted almost all his heart and soul to portray the character─Lin Daiyu, whose tragic and impressive love story has moved thousands of readers into tears. While, Charlotte Bronte ’s Jane Eyre raised heated discussion in 19th century because of the vivid and energetic female she depicted in her book. By comparison, it is easy to find that there are some similarities between the two characters. However, their fates are quite different. This paper tries to make a comparison be-tween Jane Eyre and Lin Daiyu, review their life experience, analyze possible reasons that may account for the different fates, and draw a conclusion. Hope that this paper can do some help to those who are interested in literature home and abroad.

  19. An Algorithmic Complexity Interpretation of Lin's Third Law of Information Theory

    Directory of Open Access Journals (Sweden)

    Joel Ratsaby

    2008-03-01

    Full Text Available Instead of static entropy we assert that the Kolmogorov complexity of a static structure such as a solid is the proper measure of disorder (or chaoticity. A static structure in a surrounding perfectly-random universe acts as an interfering entity which introduces local disruption in randomness. This is modeled by a selection rule R which selects a subsequence of the random input sequence that hits the structure. Through the inequality that relates stochasticity and chaoticity of random binary sequences we maintain that Lin’s notion of stability corresponds to the stability of the frequency of 1s in the selected subsequence. This explains why more complex static structures are less stable. Lin’s third law is represented as the inevitable change that static structure undergo towards conforming to the universe’s perfect randomness.

  20. Structural and electronic properties of stable Lin (n=2-10) clusters: A density functional study

    Science.gov (United States)

    Chetri, Pawan; Deka, Ramesh Ch.; Choudhury, Amarjyoti

    2013-12-01

    Structure and relative stability of Lin clusters for n=1-10 were investigated using density functional methods based DMol3 program. The structures of Li clusters were determined in terms of Li-Li bond length and the results are in very good agreement with experimental values. Stability of the clusters was determined from their relative energy values binding energies and second difference energy. We also determined fragmentation energy of each size of cluster. We arrived at some interesting result like the transition of Lin from 2-dimension to 3-dimension for a particular value of n and also the variation of HOMO-LUMO gap with cluster size. Many structures are characterized for the first time in this work.

  1. Fragmented Encounters, Social Slippages: Lin Huiyin's "In Ninety-Nine Degree Heat"

    Directory of Open Access Journals (Sweden)

    Carles Prado-Fonts

    2010-01-01

    Full Text Available The article reads Lin Huiyin’s short story “In Ninety-Nine Degree Heat” (1934 in relation to the context of 1930s China, as an innovative literary work which combines elements from both the Chinese and the Western traditions, and as a text which informs readers not only of the problematic of class and gender issues in 1930s Chinese society but also of the context of the liuxuesheng who returns to China –like Lin Huiyin herself. Focusing on questions like otherness, representation, and encounters, the essay analyzes how the episodic narrative structure of Lin’s short story echoes social and representational discourses in post-May Fourth China, at the same time that it explores issues such as social inequality, otherness and alienation, which were crucial to the liuxuesheng, and which reflect Lin’s own experience as a returned and alienated liuxuesheng at the time.

  2. Lin28 mediates radiation resistance of breast cancer cells via regulation of caspase, H2A.X and Let-7 signaling.

    Directory of Open Access Journals (Sweden)

    Linbo Wang

    Full Text Available Resistance to radiation therapy is a major obstacle for the effective treatment of cancers. Lin28 has been shown to contribute to breast tumorigenesis; however, the relationship between Lin28 and radioresistance remains unknown. In this study, we investigated the association of Lin28 with radiation resistance and identified the underlying mechanisms of action of Lin28 in human breast cancer cell lines. The results showed that the expression level of Lin28 was closely associated with resistance to radiation treatment. The T47D cancer cell line, which highly expresses Lin28, is more resistant to radiation than MCF7, Bcap-37 or SK-BR-3 cancer cell lines, which have low-level Lin28 expression. Transfection with Lin28 siRNA significantly led to an increase of sensitivity to radiation. By contrast, stable expression of Lin28 in breast cancer cells effectively attenuated the sensitivity to radiation treatment. Stable expression of Lin28 also significantly inhibited radiation-induced apoptosis. Moreover, further studies have shown that caspases, H2A.X and Let-7 miRNA were the molecular targets of Lin28. Stable expression of Lin28 and treatment with radiation induced H2AX expression, while inhibited p21 and γ-H2A.X. Overexpression of Let-7 enhanced the sensitivities to radiation in breast cancer cells. Taken together, these results indicate that Lin28 might be one mechanism underlying radiation resistance, and Lin28 could be a potential target for overcoming radiation resistance in breast cancer.

  3. Studies on the Translation Theory and Translation Practice of Lin Yutang through Moment in Peking

    Institute of Scientific and Technical Information of China (English)

    姚驰

    2014-01-01

    This paper makes studies on the translation theory and translation practice of Lin Yutang through Moment in Peking. First the paper describes Lin’s achievements and translation theories mainly from his work on translation and then associates them with his world-renowned novel Moment in Peking to testify those effective theories, indicating that translators should take on the responsibility of introducing Chinese culture and promoting the communication between countries.

  4. Studies on the Translation Theory and Translation Practice of Lin Yutang through Moment in Peking

    Institute of Scientific and Technical Information of China (English)

    姚驰

    2014-01-01

    This paper makes studies on the translation theory and translation practice of Lin Yutang through Moment in Peking.First the paper describes Lin’s achievements and translation theories mainly from his work on translation and then associates them with his world-renowned novel Moment in Peking to testify those effective theories, indicating that translators should take on the responsibility of introducing Chinese culture and promoting the communication between countries.

  5. Search for the Halo Effect in the 1H(6He, 6Li)n Peaction

    Institute of Scientific and Technical Information of China (English)

    李志宏; 柳卫平; 白希祥; 王友宝; 连钢; 李志常; 申庆彪; 林承键; 曾晟; 符长波

    2002-01-01

    The angular distributions of the charge exchange reaction 1 H(6He, 6Li)n were measured in reverse kinematics with a secondary 6He beam at the energy of4.17 AMeV. The data were analysed in the context ora microscopic calculation. It is shown that both the ground state of6He and the second excited state of6Li (3.563 MeV, 0+) have a halo structure.

  6. Professor LIAN Yu-lin's Experience in Treating Gynecological Disorders by Acupuncture

    Institute of Scientific and Technical Information of China (English)

    尚方明

    2006-01-01

    @@ Professor LIAN Yu-lin, a chief doctor in the acupuncture department of the first affiliated hospital of Tianjin College of Traditional Chinese Medicine, has been engaged himself in Chinese medicine for over 30 years with perfect medical skills and rich experience in the treatment of cerebrovascular diseases and cervical and lumbar diseases, especially in the treatment of gynecological disorders by acupuncture. I follow professor Lian as an intern and have learnt some skillful techniques in the treatment of gynecological disorders by acupuncture.

  7. Response to Niklasson's comment on Lin, et al. (2012) : "the relation between postural movement and bilateral motor integration".

    Science.gov (United States)

    Lin, Chin-Kai; Kuo, Bor-Chen; Wu, Huey-Min

    2014-10-01

    In the study of Lin, Wu, Lin, Wu, Wu, Kuo, and Yeung (2012 ), the relationship between the validity of postural movement and bilateral motor integration in terms of sensory integration theory was examined. Postural movement is the ability to use the antigravity postures required for stabilization of the neck, trunk and upper extremities via muscle co-contractions in the neck and upper extremities, and balance. Niklasson's (2013 ) comment argued that postural movement should include primitive reflexes in terms of the general abilities approach. Niklasson (2013 ) focused on the efficacy of the treatment rather than the theoretical frameworks implied in the therapeutic activities. For that purpose Lin, et al. (2012 ) used sensory integration as the theoretical foundation, and the relationship between postural movement and bilateral motor integration was assessed via empirical data. The result of Lin, et al. (2012 ) was offered as a theoretical reference for therapeutic activities.

  8. Physico-chemical evaluation of Rosmarinus officinalis L. essential oils

    Directory of Open Access Journals (Sweden)

    Ana Cristina Atti-Santos

    2005-11-01

    Full Text Available Nineteen samples of Rosmarinus officinalis were extracted by steam distillation in a pilot plant and evaluated in terms of chemical compositions and physico-chemical characteristics. The volatile oil yields ranged from 0.37% (1999 harvest to 0.49% (1998 harvest. Twenty components were identified in the oils. The major components were alpha-pinene (40.55 to 45.10%, 1,8-cineole (17.40 to 19.35%, camphene (4.73 to 6.06% and verbenone (2.32 to 3.86%. The physico-chemical parameters averaged 0.8887 g/cm³ for specific gravity, 1.4689 for refractive index, and +11.82° for optical rotation, and there were no significant variations in either the chemical or physico-chemical data in the different years.Dezenove amostras de Rosmarinus officinalis foram extraídas por destilação por arraste a vapor em uma planta piloto e os óleos essenciais foram avaliados quanto a composição química e características físico-químicas. Os rendimentos de óleo essencial variaram de 0,37% (média de 1999 a 0,49% (média de 1998. Vinte componentes foram identificados nos óleos essenciais. Os componentes majoritários foram a-pineno (40,55 a 45,10%, 1,8-cineol (17,40 a 19,35%, canfeno (4,73 a 6,06% e verbenona (2,32 a 3,86%. A média por safra dos parâmetros físico-químicos avaliados resultaram em 0,8887g/cm³ para densidade específica, 1,4689 para índice de refração e +11,82º para rotação óptica, sendo que as variações encontradas para os parâmetros químicos e físico-químicos dos óleos essenciais não variaram significativamente em função do ano de produção.

  9. Polyploid evolution in Oryza officinalis complex of the genus Oryza

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    Li Changbao

    2009-10-01

    Full Text Available Abstract Background Polyploidization is a prominent process in plant evolution, whereas the mechanism and tempo-spatial process remained poorly understood. Oryza officinalis complex, a polyploid complex in the genus Oryza, could exemplify the issues not only for it covering a variety of ploidy levels, but also for the pantropical geographic pattern of its polyploids in Asia, Africa, Australia and Americas, in which a pivotal genome, the C-genome, witnessed all the polyploidization process. Results Tracing the C-genome evolutionary history in Oryza officinalis complex, this study revealed the genomic relationships, polyploid forming and diverging times, and diploidization process, based on phylogeny, molecular-clock analyses and fluorescent in situ hybridization using genome-specific probes. Results showed that C-genome split with B-genome at ca. 4.8 Mya, followed by a series of speciation of C-genome diploids (ca. 1.8-0.9 Mya, which then partook in successive polyploidization events, forming CCDD tetraploids in ca. 0.9 Mya, and stepwise forming BBCC tetraploids between ca. 0.3-0.6 Mya. Inter-genomic translocations between B- and C-genomes were identified in BBCC tetraploid, O. punctata. Distinct FISH (fluorescent in situ hybridization patterns among three CCDD species were visualized by C-genome-specific probes. B-genome was modified before forming the BBCC tetraploid, O. malampuzhaensis. Conclusion C-genome, shared by all polyploid species in the complex, had experienced different evolutionary history particularly after polyploidization, e.g., inter-genomic exchange in BBCC and genomic invasion in CCDD tetraploids. It diverged from B-genome at 4.8 Mya, then participated in the tetraploid formation spanning from 0.9 to 0.3 Mya, and spread into tropics of the disjunct continents by transcontinentally long-distance dispersal, instead of vicariance, as proposed by this study, given that the continental splitting was much earlier than the C

  10. Comparative Embryological Studies on Infertility of Interspecific Hybridizations Between Oryza sativa with Different Ploidy Levels and O.officinalis

    Institute of Scientific and Technical Information of China (English)

    FU Xue-lin; LU Yong-gen; LIU Xiang-dong; LI Jin-quan; ZHAO Xing-juan

    2009-01-01

    As maternal parents, diploid (L202-2x) and autotetraploid (L202-4x) of Oryza sativa cv. L202 were crossed with O. officinalis. Embryo development and fertilization in these two crosses were comparatively studied. There were no mature hybrid seeds obtained because all the hybridized spikelets died 30 days after pollination. The main reasons for no seed set were abnormal fertilization and development of the embryos and endosperms in the interspecific hybrids. There were double- fertilization, egg cell single-fertilization and non-fertilization in these crosses. Although 59.45% and 54.87% of hybrid embryos produced in the crosses of L202-2x/O. officinalis and L202-4x/O. officinalis, respectively, hybrid embryos ceased to develop or degenerated and plenty of free endosperm nuclei were in disaggregating state without developing cellular endosperms three days after pollination. Besides, some embryological differences in these two crosses were found, that is, the rate of double-fertilization and total rate of double- and single-fertilization in L202-2x/O. officinalis were higher than those in L202-4x/O. officinalis. The embryo and endosperm of hybrids developed more slowly, and embryos and free endosperm nuclei were more severely degenerated in L202-4x/O. officinalis than in L202-2x/O. officinalis. Five days after pollination, a few of embryos in L202-2x/O. officinalis developed into pear-shaped ones, however, embryos in L202-4x/O. officinalis were all degenerated. Therefore, it is more difficult to obtain interspecific hybrids by wide crosses between autotetraploid of O. sativa and O. officinalis.

  11. Dynamiques complexes et morphogenèse Introduction aux sciences non linéaires

    CERN Document Server

    Misbah, Chaouqi

    2011-01-01

    Les sciences non linéaires ont pour objet l’ensemble des phénomènes dont l’analyse résiste au principe de superposition. Elles concernent en grande partie les systèmes dits « complexes » dont l’interaction et l’interdépendance entre les parties empêchent de prédire précisément l’évolution du système. Pour expliquer ces phénomènes, deux approches complémentaires ont été proposées : la théorie des bifurcations et la théorie des catastrophes. Mais la pleine compréhension et la modélisation de la non-linéarité restent chacune un défi pour les scientifiques du XXIe siècle. C’est dans la perspective d’accompagner tous ceux qui voudront le relever que ce livre a été conçu. Son objectif est d’exposer au lecteur le langage et le formalisme nécessaires à l’étude de la non-linéarité. Partant d’exemples simples, pour ensuite atteindre un niveau d’abstraction visant l’universalité, l’auteur explore les divers scénarios possibles de bifurcations et les catastr...

  12. Differential regulation of the c-Myc/Lin28 axis discriminates subclasses of rearranged MLL leukemia

    Science.gov (United States)

    Chen, Lili; Sun, Yuqing; Wang, Jingya; Jiang, Hui; Muntean, Andrew G.

    2016-01-01

    MLL rearrangements occur in myeloid and lymphoid leukemias and are generally associated with a poor prognosis, however this varies depending on the fusion partner. We modeled acute myeloid leukemia (AML) in mice using various MLL fusion proteins (MLL-FPs) and observed significantly different survival outcomes. To better understand the differences between these leukemias, we examined the genome wide expression profiles of leukemic cells transformed with different MLL-FPs. RNA-sequencing and pathway analysis identified the c-Myc transcriptional program as one of the top distinguishing features. c-Myc protein levels were highly correlative with AML disease latency in mice. Functionally, overexpression of c-Myc resulted in a more aggressive proliferation rate in MLL-FP cell lines. While all MLL-FP transformed cells displayed sensitivity to BET inhibitors, high c-Myc expressing cells showed greater resistance to Brd4 inhibition. The Myc target Lin28B was also differentially expressed in MLL-FP cell lines in agreement with c-Myc expression. Examination of Lin28B miRNAs targets revealed that let-7g was significantly increased in leukemic cells associated with the longest disease latency and forced let-7g expression induced differentiation of leukemic blasts. Thus, differential regulation of the c-Myc/Lin28/let-7g program by different MLL-FPs is functionally related to disease latency and BET inhibitor resistance in MLL leukemias. PMID:27007052

  13. Growth of Jasminum sambac, Calendula officinalis, Matthiola incana, and Senecio hybridus Influenced by High-Voltage Electrostatic Field%高压静电场对茉莉等四种花卉的调控作用

    Institute of Scientific and Technical Information of China (English)

    蒋耀庭; 潘丽娜

    2003-01-01

    采用本部研制的直流高压脉冲器定时对茉莉花、金盏菊、紫罗兰和瓜叶菊进行处理.结果表明.花的开花时间均得到延长。除茉莉花的开花量有所减少外.其余三种花的开花量均有所增加。

  14. 不同浓度氯化钠胁迫对金盏菊生长发育的影响%Effect of Different Concentration of NaCl Stress on the Growth and Development of Calendula officinalis

    Institute of Scientific and Technical Information of China (English)

    高慧; 陈梦玲; 朱小燕

    2013-01-01

    以金盏菊为试材,研究了不同浓度NaCl(40、80、120、160、200 mmol/L)胁迫对金盏菊生长和生理特性的影响.结果表明:在不同浓度NaCl胁迫下,金盏菊的株高、茎粗、叶片数、叶片和根的鲜重与干重、叶绿素含量、蛋白质含量和可溶性糖含量均呈先上升后下降的趋势,而根长和根体积呈逐渐下降趋势.表明金盏菊耐盐阈值80 mmol/L.

  15. 金盏菊总黄酮提取工艺研究及含量测定%Study on Extraction Technology and Content Determination of Total Flavonoids from Calendula officinalis.L.

    Institute of Scientific and Technical Information of China (English)

    郑佳; 卢先明; 易超宇; 高必兴

    2014-01-01

    目的:优选金盏菊总黄酮的提取工艺,建立金盏菊总黄酮含量测定的方法.方法:以金盏菊总黄酮提取率为指标,通过单因素考察,研究金盏菊总黄酮的最佳提取工艺;并利用紫外分光光度法,以芦丁为对照品,对10个不同产地以及同一产地不同采收期的金盏菊总黄酮进行含量测定.结果:金盏菊总黄酮最佳提取工艺为:料液比1∶50,提取温度90℃,60%乙醇回流提取1.5h;10个不同产地的金盏菊总黄酮含量为2.25%~4.78%,平均含量为3.17%;初花期的金盏菊总黄酮含量最高,为3.58%.结论:建立了金盏菊总黄酮含量测定方法,此方法简单、准确、稳定、可靠,可作为金盏菊总黄酮的质量控制.

  16. Study on the optimum extraction condition of carotenoids from calendula officinalis L. Flowers%金盏菊花类胡萝卜素提取最佳条件的研究

    Institute of Scientific and Technical Information of China (English)

    胡晓丹; 盛群刚; 谢笔钧

    2001-01-01

    在不同溶剂、提取时间、温度、料液比和次数等条件下,通过正交实验,对金盏菊花类胡萝卜素提取最佳条件进行了研究.结果表明:最佳提取工艺条件是用石油醚-丙酮(1:1,V/V)混合溶剂浸提,料液比为1:25(g/ml),在45℃浸提1 h,反复4次,浸提率可达93.5%.

  17. 不同浓度钾对金盏菊生长发育的影响%THE EFFECT OF DIFFERENT POTASSIUM CONCENTRATION ON Calendula officinalis PLANTS GROWIH AND DEVELOPMENT

    Institute of Scientific and Technical Information of China (English)

    常丽新

    2000-01-01

    为了给金盏菊的规模化无土栽培提供参考依据,采用日本园式营养液配方,探讨不同浓度钾对金盏菊生长发育的影响,对金盏菊植株叶片数、株高、茎粗、鲜重、干物质重量和开花情况进行了分析.结果表明,营养液中钾浓度为400 mg/L时,最适宜金盏菊的生长发育,金盏菊生长对缺钾比较敏感,但对高浓度钾不太敏感.

  18. 金盏菊总皂苷提取及其抗氧化、降血脂作用研究%Extraction of Total Saponins from Calendula Officinalis L and its Effects of Anti-oxidation & Hypolipidemic

    Institute of Scientific and Technical Information of China (English)

    刘丽; 国兴明

    2010-01-01

    通过对金盏菊总皂苷的实验室提取工艺及其抗氧化、降血脂作用研究,结果表明:金盏菊总皂苷较为理想的实验室提取工艺为:提取温度75℃,提取时间55 min,乙醇浓度75%,料液比1:35.在此条件下,总皂苷的提取率为2.01%,总固物中总皂苷含量达到35.4%.金盏菊总皂苷对O2·、·OH皆表现出较好的清除效果,清除率分别达到47.29%和88.42%;小鼠血清中的TC、TC、LDL-C与模型组相比,均有一定程度的下降.

  19. Extraction and Stability of Pigment from Calendula officinalis L.Flowers%金盏菊花中色素的提取工艺及稳定性研究

    Institute of Scientific and Technical Information of China (English)

    李泽鸿; 张晓刚; 张璐; 韩娟; 张帆; 刘洪章

    2011-01-01

    目的:确定金盏菊花色素的最佳提取工艺,并对其稳定性进行研究.方法:以浸提法对金盏菊花中的色素进行提取,通过对提取剂、料液比、提取温度、提取时间4因素进行,L9(3~4)正交试验得到最佳提取条件;同时考察光照、热和食品添加剂等对色素稳定性的影响.结果:金盏菊花色素最佳提取工艺为提取剂90%乙醇、料液比1:8(g/mL)、在70℃水浴中浸提40min;该色素在酸性条件下稳定性较好,对光和热稳定性好;pH值对色素的稳定性影响较大,在酸性条件(pH5)下该色素较稳定;常用食品添加剂如葡萄糖、蔗糖对色素的色泽无不良影响,金属离子Na+、Mg2+、Ca2+、Cu2+对金盏菊花色素无不良影响,而Fe3+则对色素有明显影响.结论:获得金盏菊花中色素提取的最佳工艺;色素对光、热、常用食品添加剂的稳定性良好,为其在食品和药品中的应用提供了广阔的前景.

  20. 灯盏细辛注射液治疗脑梗死30例疗效验证观测%Effects Of Calendula Officinalis L Injectio on Cerebral Infarction: A Report of 30 cases

    Institute of Scientific and Technical Information of China (English)

    何群; 陈国强; 毕清

    2005-01-01

    目的验证观测灯盏细辛治疗脑梗死的疗效.方法 60例脑梗死患者随机分为A、B两组.A组用灯盏细辛注射液20ml,1次/d,共15d.B组:用丹参注射液20ml,1次/d,共15d.结果 A组有效28例,总有效率 93.33%,B组有效22例,总有效率73.33%.两组经卡方检验,χ2=23.145,χ2(0.01)=6.63,P<0.01,两组间效率有非常显著差异.结论灯盏细辛是治疗脑梗死有效的制剂.

  1. 城市生活污水浇灌对金盏菊生长的影响%Effects of Municipal Domestic Sewage Effluents on the Growth of Calendula officinalis

    Institute of Scientific and Technical Information of China (English)

    刘俊伟; 胡宏友; 许甘治; 陈登雄; 卢昌义

    2005-01-01

    用三种稀释倍数的城市生活污水[污水∶清水(v/v)为1﹕0、1﹕0.5、1﹕1]处理金盏菊种子及盆花,结果表明,污灌处理不仅显著地降低种子的萌发率,还增加萌发后幼苗病害的发生率;未经稀释的原生污水显著抑制金盏菊幼苗的生长,而稀释后抑制作用降低,当稀释至适当浓度时,则对幼苗生长起促进作用;对于金盏菊成年植株,污灌处理(不论稀释与否)显著地增加其茎、叶和根的鲜重,分别较对照增加161.63 %~215.12 %、86.77 %~109.23 %和23.89 %~34.13 %.综合分析表明,原生污水经过适当稀释后用于草花污灌,可以使污水中的营养盐得到回用,提高草花的观赏性.

  2. Effects of Drought Stress on Membrane Lipid Peroxidation and Protective Enzymes Activity in Calendula officinalis%干旱胁迫对金盏菊膜脂过氧化及保护酶活性的影响

    Institute of Scientific and Technical Information of China (English)

    谷文众; 刘杨; 谷振军

    2009-01-01

    为给金盏菊栽培技术的改进及新种选育提供理论依据,在人工模拟持续干旱条件下,研究了膜脂过氧化及保护酶活性等生理指标的变化.研究结果表明,金盏菊叶片细胞1 d内质膜透性增大,渗透调节物质和膜脂过氧化产物也相应增加,3~5 d内增大趋势不明显,其实质是POD和CAT活性先上升后下降,特别是干旱维持7 d时CAT活性低于对照水平.

  3. Effect of Calendula officinalis L.Extracts on Performance and Egg Qualities of Laying Hens%金盏菊提取物对蛋鸡生产性能和蛋品质的影响

    Institute of Scientific and Technical Information of China (English)

    高杨; 陈辉; 黄仁录; 王翠菊; 杨秋霞; 王学强

    2010-01-01

    为研究日粮中添加不同水平金盏菊提取物对蛋鸡生产性能和蛋品质的影响,试验选取400日龄海兰灰蛋鸡224只,随机分成4组,每组4个重复,每个重复14只,金盏菊提取物添加水平分别为0、1、2、4 g/kg,试验期14 d.结果:蛋鸡日粮中添加金盏菊提取物对蛋鸡产蛋率、平均蛋重、平均耗料量、料蛋比、蛋壳厚度、蛋壳相对重和蛋黄比率均无显著影响(P>0.05);哈氏单位随着金盏菊提取物添加量的增加逐渐增加,添加剂量为2、4 g/kg试验组与对照组相比差异显著(P<0.05);蛋黄颜色自第2天起,各试验组与对照组相比逐渐加深,试验第5天,2、4g/kg的试验组蛋黄颜色达到显著水平(P<0.05),到第8天达到极显著水平(P<0.01);其中添加金盏菊提取物剂量达到2 g/kg时,蛋黄颜色极显著提高(P<0.01).结果提示,2 g/kg金盏菊提取物为适宜添加量.

  4. Influence of Salt Stress on Germination Characteristics of Celosia Cristata and Calendula officinalis Seeds%NaCl胁迫对鸡冠花和金盏菊种子发芽特性的影响

    Institute of Scientific and Technical Information of China (English)

    贾双双; 高青海; 张雪平; 胡景浪

    2013-01-01

    以鸡冠花、金盏菊两种花卉种子为试验材料,研究不同浓度NaCl溶液(0、50、100、150、200、250mmol/L)胁迫下种子发芽率、相对发芽率、发芽势、主根长、下胚轴长、侧根长、幼芽叶绿素含量及根系活力等相关指标的变化规律.试验结果表明:鸡冠花种子在NaCl浓度≤100 mmol/L时,各指标与对照差异不显著,说明鸡冠花对低浓度盐胁迫有一定适应性,而高浓度的盐胁迫对其有很强抑制作用;随着NaCl浓度升高,金盏菊各项测量指标均受到一定程度抑制.说明不同露地花卉种子在发芽期的耐盐力表现出明显的差异,且鸡冠花耐盐能力显著强于金盏菊.

  5. Effect of intercropping carrot (Daucus carota L.) with french marigold (Tagetes patula nana L.) and pot marigold (Calendula officinalis L.) on the occurrence of some pests and quality of carrot yield

    OpenAIRE

    Beata Jankowska; Elżbieta Jędrszczyk; Małgorzata Poniedziałek

    2012-01-01

    Intercropping combines different aspects of the interaction between organisms in ecosystems and may be classified as a pro-ecological method of plant cultivation limiting the harmful human interference in the environment, especially the use of chemicals. It also allows high yield, good quality, and economic productivity to be achieved. The aim of the present study, conducted in the years 2003–2004 in Mydlniki near Kraków, was to determine the effect of intercropping carrot Nardin F1 with Fren...

  6. Biosynthesis of flat silver nanoflowers: from Flos Magnoliae Officinalis extract to simulation solution

    Science.gov (United States)

    Jing, Xiaolian; Huang, Jiale; Wu, Lingfeng; Sun, Daohua; Li, Qingbiao

    2014-03-01

    Flat Ag nanoflowers were directly synthesized from the bioreduction of AgNO3 using Flos Magnoliae Officinalis extract without any additional stabilizer or protective agent at room temperature. Effects of concentrations of the Flos Magnoliae Officinalis extract on the Ag nanostructures were investigated. The main components containing flavone, polyphenol, protein, and reducing sugar in the plant extract were thoroughly determined before and after the reaction, and the dialysis experiments were also conducted. The results of components analysis and dialysis showed that gallic acid representing polyphenols played an important role in the biosynthesis of silver nanoplates. Trisodium citrate combined gallic acid solution, instead of Flos Magnoliae Officinalis extract, was employed and successfully simulated the biosynthesis process of the flat Ag nanoflowers.

  7. A new extraction method of bioflavanoids from poisonous plant (Gratiola Officinalis L.

    Directory of Open Access Journals (Sweden)

    Natalya V. Polukonova

    2014-09-01

    Full Text Available The way of vegetable raw materials extraction which allows to receive nontoxical composition of biological active agents from poisonous plants such as Gratiola officinalis L. was described. The alkaloids exit changes with the increase of ethyl alcohol percentage (from 15% to 96%. The extract was obtained using 96% ethanol and did not give positive high quality reaction to the content of alkaloids. The chemical composition with new nontoxical biological active composition of Gratiola officinalis L. extract was investigated. The extract contains a previously unknown plant – bioflavonoid quercetin. The average value of quercetin in this extract using the calibration curve of the standard sample quercetin (98% Sigma is 0.66%. In the dry rest of extractive substances (Gratiola officinalis L. the quantity of quercetin was 350 mkg (obtained from 10 g of a dry grass as was established by the method of a liquid chromatography.

  8. ESTs library from embryonic stages reveals tubulin and reflectin diversity in Sepia officinalis (Mollusca — Cephalopoda).

    Science.gov (United States)

    Bassaglia, Yann; Bekel, Thomas; Da Silva, Corinne; Poulain, Julie; Andouche, Aude; Navet, Sandra; Bonnaud, Laure

    2012-05-01

    New molecular resources regarding the so-called “non-standard models” in biology extend the present knowledge and are essential for molecular evolution and diversity studies (especially during the development) and evolutionary inferences about these zoological groups, or more practically for their fruitful management. Sepia officinalis, an economically important cephalopod species, is emerging as a new lophotrochozoan developmental model. We developed a large set of expressed sequence tags (ESTs) from embryonic stages of S. officinalis, yielding 19,780 non-redundant sequences (NRS). Around 75% of these sequences have no homologs in existing available databases. This set is the first developmental ESTs library in cephalopods. By exploring these NRS for tubulin, a generic protein family, and reflectin, a cephalopod specific protein family,we point out for both families a striking molecular diversity in S. officinalis.

  9. The histopathological effects of salvia officinalis on the kidney and liver of rats

    Directory of Open Access Journals (Sweden)

    D.A. Adekomi

    2013-03-01

    Full Text Available The aim of this investigation was to evaluate some of the effects of aqueous leaf extract of Salvia officinalis on the kidney and liver of male Sprague Dawley rats. Ten Sprague-Dawley rats (7-11 weeks old were randomly assigned into two groups; A and B. Aqueous extract of S. officinalis leaves (300 mg/kg body weight was administered orally to the rats in group B while the rats in group A received equal volume of normal saline for 14d. At termination of treatment, the histopathology of the kidney and liver were assessed. The kidney and the liver in the extract treated rat displayed organized and preserved histological profile. Our findings suggest that S. officinalis has no deleterious effects on the kidney and liver of the rats.  

  10. Acute atropine intoxication with psychiatric symptoms by herbal infusion of Pulmonaria officinalis (Lungwort

    Directory of Open Access Journals (Sweden)

    Enrique Baca-García

    2007-06-01

    Full Text Available Background and objectives: Lungwort infusion is a preparation extracted from Pulmonaria officinalis which is occasionally used as a folk remedy for the common cold. The current report aims to describe acute atropine intoxications with delirium caused by Lungwort infusion in several members of the same family. Methods: Description of three case reports. Search of literature through Medline. Results: Three generations of a same family presented acute and moderately severe atropine intoxications after drinking an infusion prepared with Pulmonaria officinalis. Conclusions: Despite the lack of scientific evidence for its clinical use, medicinal plants continue being widely used. In spite of severe adverse effects reported, the general thought is that herbal remedies are harmless. To our knowledge, this is the first report of acute atropine intoxications with psychiatric symptoms secondary to Pulmonaria officinalis in several members of a family. We suspect that the lungwort infusion may have been contaminated with some other substance with atropinic properties.

  11. High LIN28A Expressing Ovarian Cancer Cells Secrete Exosomes That Induce Invasion and Migration in HEK293 Cells.

    Science.gov (United States)

    Enriquez, Vanessa A; Cleys, Ellane R; Da Silveira, Juliano C; Spillman, Monique A; Winger, Quinton A; Bouma, Gerrit J

    2015-01-01

    Epithelial ovarian cancer is the most aggressive and deadly form of ovarian cancer and is the most lethal gynecological malignancy worldwide; therefore, efforts to elucidate the molecular factors that lead to epithelial ovarian cancer are essential to better understand this disease. Recent studies reveal that tumor cells release cell-secreted vesicles called exosomes and these exosomes can transfer RNAs and miRNAs to distant sites, leading to cell transformation and tumor development. The RNA-binding protein LIN28 is a known marker of stem cells and when expressed in cancer, it is associated with poor tumor outcome. We hypothesized that high LIN28 expressing ovarian cancer cells secrete exosomes that can be taken up by nontumor cells and cause changes in gene expression and cell behavior associated with tumor development. IGROV1 cells were found to contain high LIN28A and secrete exosomes that were taken up by HEK293 cells. Moreover, exposure to these IGROV1 secreted exosomes led to significant increases in genes involved in Epithelial-to-Mesenchymal Transition (EMT), induced HEK293 cell invasion and migration. These changes were not observed with exosomes secreted by OV420 cells, which contain no detectable amounts of LIN28A or LIN28B. No evidence was found of LIN28A transfer from IGROV1 exosomes to HEK293 cells.

  12. Novel localisation and possible function of LIN7 and IRSp53 in mitochondria of HeLa cells.

    Science.gov (United States)

    Ferrari, Ilaria; Crespi, Arianna; Fornasari, Diego; Pietrini, Grazia

    2016-08-01

    By means of immunofluorescence and subcellular fractionation experiments, we here demonstrate mitochondrial distribution of LIN7 and IRSp53 in HeLa cells. These peripheral proteins displayed a tight association with mitochondria and coimmunoprecipitated from mitochondrial fractions. In line with a role for LIN7 in the regulation of IRSp53 activity on actin dynamics, the morphology of mitochondria was similarly altered by changing the expression levels of either each protein or both, whereas mitochondrial morphology was preserved in cells overexpressing IRSp53 deleted of its binding domains for LIN7 (IRSp53Δ5) or for actin polymerisation modulators (IRSp53ΔSH3). In particular, the overexpression of full length LIN7 and/or IRSp53 increased the percentage of cells with short mitochondria, while downregulation of the endogenous proteins by shRNAs increased the amount of cells with elongated and perinuclear clustered mitochondria. These mitochondria were only partially resistant to fragmentation induced by dissipation of the mitochondrial membrane potential (i.e. treatment with sodium azide), whereas mitochondria were fully protected by the fission defective mutant Drp1 K38A. Overexpression of LIN7 or IRSp53 did not prevent the formation of hyperfused mitochondria in cells coexpressing the Drp1 K38A mutant, thus suggesting that LIN7-IRSp53 complex requires functional Drp1 to regulate mitochondrial morphology.

  13. Makorin ortholog LEP-2 regulates LIN-28 stability to promote the juvenile-to-adult transition in Caenorhabditis elegans.

    Science.gov (United States)

    Herrera, R Antonio; Kiontke, Karin; Fitch, David H A

    2016-03-01

    The heterochronic genes lin-28, let-7 and lin-41 regulate fundamental developmental transitions in animals, such as stemness versus differentiation and juvenile versus adult states. We identify a new heterochronic gene, lep-2, in Caenorhabditis elegans. Mutations in lep-2 cause a delay in the juvenile-to-adult transition, with adult males retaining pointed, juvenile tail tips, and displaying defective sexual behaviors. In both sexes, lep-2 mutants fail to cease molting or produce an adult cuticle. We find that LEP-2 post-translationally regulates LIN-28 by promoting LIN-28 protein degradation. lep-2 encodes the sole C. elegans ortholog of the Makorin (Mkrn) family of proteins. Like lin-28 and other heterochronic pathway members, vertebrate Mkrns are involved in developmental switches, including the timing of pubertal onset in humans. Based on shared roles, conservation and the interaction between lep-2 and lin-28 shown here, we propose that Mkrns, together with other heterochronic genes, constitute an evolutionarily ancient conserved module regulating switches in development.

  14. Translating Chinese Place Names into English: The Case of Lin Yutang’s Version of Fusheng Liuji

    Directory of Open Access Journals (Sweden)

    Shuangchang Cui

    2016-04-01

    Full Text Available Chapter Four of Fusheng Liuji (Six Chapters of a Floating Life, a unique autobiographical prose work by early Qing-dynasty author Shen Fu (1763-1825, records Shen's travel to many parts of the country and contains a multitude of place names, whose rich cultural connotations pose a great challenge to the translator. Drawing on Javier Franco Aixelá’s taxonomy of translation strategies for treating culture-specific items, this paper attempts to describe the translation strategies adopted by world-renowned writer and translator Lin Yutang to handle these place names. An analysis of twenty-four examples indicates that Lin tended to preserve Chinese culture carried by the place names through employing cultural conservation strategies, including linguistic translation, transliteration, transliteration plus linguistic translation, transliteration plus annotation, and linguistic translation plus annotation. Furthermore, Lin occasionally resorted to such cultural substitution strategies as absolute universalization and domestication. It is concluded that on the whole Lin adopted a source-oriented approach to translation when working on Shen Fu's work. This study sheds fresh light on Lin's selection of translation strategies in the early years of his lifelong career as “a native interpreter of Chinese culture for Western readers”. Keywords: Place Names, Chinese Culture, Lin Yutang, Translation Strategies

  15. Protective effect of Emblica officinalis (amla) on isoproterenol-induced cardiotoxicity in rats.

    Science.gov (United States)

    Ojha, Shreesh; Golechha, Mahaveer; Kumari, Santosh; Arya, Dharamvir Singh

    2012-06-01

    Emblica officinalis, commonly known as amla, is an important medicinal plant reputed for its dietary and therapeutic uses. The aim of the present study was to investigate the protective role of E. officinalis against isoproterenol (ISP)-induced cardiotoxicity in rats and elucidate the possible mechanism involved. Rats were administered E. officinalis (100, 250 and 500 mg/kg, p.o.) or vehicle (normal saline) for 30 days, with concurrent subcutaneous injections of ISP (85 mg/kg, at 24 h interval) on 29th and 30th day. ISP-induced cardiac dysfunction as evidenced by decreased mean arterial pressure, heart rate, contractility (+LVdP/dt) and relaxation (-LVdP/dt) along with increased left ventricular end diastolic pressure. ISP significantly (p < 0.05) decreased antioxidant enzymes, superoxide dismutase, catalase and glutathione peroxidase and myocyte-injury-specific marker enzymes, creatine phosphokinase-MB and lactate dehydrogenase in heart. A significant (p < 0.05) depletion of reduced glutathione and increase in thiobarbituric acid reactive substances along with histopathological alteration has further indicated the oxidative damage of myocardium. However, pretreatment with E. officinalis exhibited restoration of hemodynamic and left ventricular function along with significant preservation of antioxidants, myocytes-injury-specific marker enzymes and significant inhibition of lipid peroxidation. Furthermore, histopathological salvage of myocardium reconfirmed the protective effects of E. officinalis. Results of the present study demonstrate cardioprotective potential of E. officinalis attributed to its potent antioxidant and free radical scavenging activity as evidenced by favorable improvement in hemodynamic, contractile function and tissue antioxidant status.

  16. Aqueous extract of Rosmarinus officinalis L. inhibits neutrophil influx and cytokine secretion.

    Science.gov (United States)

    Silva, Ana Mara de Oliveira E; Machado, Isabel Daufenback; Santin, José Roberto; de Melo, Illana Louise Pereira; Pedrosa, Gabriela Vieira; Genovese, Maria Ines; Farsky, Sandra Helena Poliselli; Mancini-Filho, Jorge

    2015-01-01

    Rosmarinus officinalis L. phenolic compounds have attracted considerable attention because of their antioxidant and antimicrobial properties, including its ability to treat inflammatory disorders. In this work, we investigated the in vivo and in vitro effects of R. officinalis aqueous extract on neutrophil trafficking from the blood into an inflamed tissue, on cell-derived secretion of chemical mediators, and on oxidative stress. Anti-inflammatory activity was investigated using carrageenan-induced inflammation in the subcutaneous tissue of male Wistar rats orally treated with the R. officinalis extract (100, 200, or 400 mg/kg). The leukocyte influx (optical microscopy), secretion of chemical mediators (prostaglandin E2 (PGE2), TNF-α, interleukin 6 (IL-6), leukotriene B4 (LTB4), and cytokine-induced neutrophil chemoattractant 1 by enzyme-linked immunosorbent assay), and the anti-oxidative profile (super oxide dismutase (SOD), glutathione peroxidase, and thiobarbituric acid reactive substance (TBARS) spectrophotometry) were quantified in the inflamed exudate. N-Formyl-methionine-leucine-phenylalanine-induced chemotaxis, lipopolysaccharide-induced NO2 (-) production (Greiss reaction), and adhesion molecule expression (flow cytometry) were in vitro quantified using oyster glycogen recruited peritoneal neutrophils previous treated with the extract (1, 10, or 100 µg/mL). Animals orally treated with phosphate-buffered saline and neutrophils incubated with Hank's balanced salt solution were used as control. R. officinalis extract oral treatment caused a dose-dependent reduction in the neutrophil migration as well as decreased SOD, TBARS, LTB4, PGE2, IL-6, and TNF-α levels in the inflamed exudate. In vitro treatment with R. officinalis decreased neutrophil chemotaxis, NO2 (-) production, and shedding of L-selectin and β2 integrin expressions. Results here presented show that R. officinalis aqueous extract displays important in vivo and in vitro anti

  17. Genome-wide identification and validation of simple sequence repeats (SSRs) from Asparagus officinalis.

    Science.gov (United States)

    Li, Shufen; Zhang, Guojun; Li, Xu; Wang, Lianjun; Yuan, Jinhong; Deng, Chuanliang; Gao, Wujun

    2016-06-01

    Garden asparagus (Asparagus officinalis), an important vegetable cultivated worldwide, can also serve as a model dioecious plant species in the study of sex determination and sex chromosome evolution. However, limited DNA marker resources have been developed and used for this species. To expand these resources, we examined the DNA sequences for simple sequence repeats (SSRs) in 163,406 scaffolds representing approximately 400 Mbp of the A. officinalis genome. A total of 87,576 SSRs were identified in 59,565 scaffolds. The most abundant SSR repeats were trinucleotide and tetranucleotide, accounting for 29.2 and 29.1% of the total SSRs, respectively, followed by di-, penta-, hexa-, hepta-, and octanucleotides. The AG motif was most common among dinucleotides and was also the most frequent motif in the entire A. officinalis genome, representing 14.7% of all SSRs. A total of 41,917 SSR primers pairs were designed to amplify SSRs. Twenty-two genomic SSR markers were tested in 39 asparagus accessions belonging to ten cultivars and one accession of Asparagus setaceus for determination of genetic diversity. The intra-species polymorphism information content (PIC) values of the 22 genomic SSR markers were intermediate, with an average of 0.41. The genetic diversity between the ten A. officinalis cultivars was low, and the UPGMA dendrogram was largely unrelated to cultivars. It is here suggested that the sex of individuals is an important factor influencing the clustering results. The information reported here provides new information about the organization of the microsatellites in A. officinalis genome and lays a foundation for further genetic studies and breeding applications of A. officinalis and related species.

  18. A simple and efficient DNA isolation method for Salvia officinalis.

    Science.gov (United States)

    Aleksić, Jelena M; Stojanović, Danilo; Banović, Bojana; Jančić, Radiša

    2012-12-01

    We report an efficient, simple, and cost-effective protocol for the isolation of genomic DNA from an aromatic medicinal plant, common sage (Salvia officinalis L.). Our modification of the standard CTAB protocol includes two polyphenol adsorbents (PVP 10 and activated charcoal), high NaCl concentrations (4 M) for removing polysaccharides, and repeated Sevag treatment to remove proteins and other carbohydrate contaminants. The mean DNA yield obtained with our Protocol 2 was 330.6 μg DNA g(-1) of dry leaf tissue, and the absorbance ratios 260/280 and 260/230 nm averaged 1.909 and 1.894, respectively, revealing lack of contamination. PCR amplifications of one nuclear (26S rDNA) and one chloroplast (rps16-trnK) locus indicated that our DNA isolation protocol may be used in common sage and other aromatic and medicinal plants containing essential oil for molecular biologic and biotechnological studies and for population genetics, phylogeographic, and conservation surveys in which nuclear or chloroplast genomes would be studied in large numbers of individuals.

  19. Rosmarinus officinalis extract suppresses Propionibacterium acnes-induced inflammatory responses.

    Science.gov (United States)

    Tsai, Tsung-Hsien; Chuang, Lu-Te; Lien, Tsung-Jung; Liing, Yau-Rong; Chen, Wei-Yu; Tsai, Po-Jung

    2013-04-01

    Propionibacterium acnes is a key pathogen involved in the progression of acne inflammation. The development of a new agent possessing antimicrobial and anti-inflammatory activity against P. acnes is therefore of interest. In this study, we investigated the inhibitory effect of rosemary (Rosmarinus officinalis) extract on P. acnes-induced inflammation in vitro and in vivo. The results showed that ethanolic rosemary extract (ERE) significantly suppressed the secretion and mRNA expression of proinflammatory cytokines, including interleukin (IL)-8, IL-1β, and tumor necrosis factor-α in P. acnes-stimulated monocytic THP-1 cells. In an in vivo mouse model, concomitant intradermal injection of ERE attenuated the P. acnes-induced ear swelling and granulomatous inflammation. Since ERE suppressed the P. acnes-induced nuclear factor kappa-B (NF-κB) activation and mRNA expression of Toll-like receptor (TLR) 2, the suppressive effect of ERE might be due, at least partially, to diminished NF-κB activation and TLR2-mediated signaling pathways. Furthermore, three major constituents of ERE, carnosol, carnosic acid, and rosmarinic acid, exerted different immumodulatory activities in vitro. In brief, rosmarinic acid significantly suppressed IL-8 production, while the other two compounds inhibited IL-1β production. Further study is needed to explore the role of bioactive compounds of rosemary in mitigation of P. acnes-induced inflammation.

  20. Salvia Officinalis and Cisplatin Effects on Pentylenetetrazole Induced Seizure Threshold

    Directory of Open Access Journals (Sweden)

    Mir Hadi Khayate-Nouri

    2013-11-01

    Full Text Available Background: Studies have shown that cisplatin have neuropathic effects and Salvia officinalis (SO could have therapeutic effects on nervous system. The aim of this study was to investigate the effects of SO hydroalcoholic extract and cisplatin on pentylenetetrazole (PTZ induced seizure in mice. Materials and methods: This is an experimental interventional study. For this purpose first group received normal saline, second group received SO extract, third group received cisplatin, in the fourth group received SO extract plus cisplatin and the subsequent seizure threshold was determined for each group. Results: The results showed that SO extract significantly (p<0.05 increased and in cisplatin group significantly (p<0.05 decreased seizure threshold. Simultaneous uses of cisplatin and SO extract caused to significantly increased seizure threshold (p<0.05 compared with cisplatin group. Conclusion: Considering different types of ingredients in SO extract which have beneficial effects on nervous system, it might be used to reduce cisplatin induced neuropathic effects. It seems that SO extract could be useful in cisplatin-induced seizure but further investigations are needed.

  1. Rosmarinus Officinalis Leaves as a Natural Source of Bioactive Compounds

    Directory of Open Access Journals (Sweden)

    Isabel Borrás-Linares

    2014-11-01

    Full Text Available In an extensive search for bioactive compounds from plant sources, the composition of different extracts of rosemary leaves collected from different geographical zones of Serbia was studied. The qualitative and quantitative characterization of 20 rosemary (Rosmarinus officinalis samples, obtained by microwave-assisted extraction (MAE, was determined by high performance liquid chromatography coupled to electrospray quadrupole-time of flight mass spectrometry (HPLC–ESI-QTOF-MS. The high mass accuracy and true isotopic pattern in both MS and MS/MS spectra provided by the QTOF-MS analyzer enabled the characterization of a wide range of phenolic compounds in the extracts, including flavonoids, phenolic diterpenes and abietan-type triterpenoids, among others. According to the data compiled, rosemary samples from Sokobanja presented the highest levels in flavonoids and other compounds such as carnosol, rosmaridiphenol, rosmadial, rosmarinic acid, and carnosic acid. On the other hand, higher contents in triterpenes were found in the extracts of rosemary from Gložan (Vojvodina.

  2. Influence of light intensity on growth and physiological characteristics of common sage (Salvia officinalis L.

    Directory of Open Access Journals (Sweden)

    George Zervoudakis

    2012-02-01

    Full Text Available The aim of this work was to investigate the effects of four different light intensities on the growth characteristics, physiological parameters and leaf photosynthetic pigments of Salvia officinalis L. The plant's dry mass, number of the leaves and physiological parameters indicated a strong positive correlation with the light intensity. On the other hand, the plant's height and leaf photosynthetic pigments were increased at low light treated plants. These results suggest that the aromatic herb Salvia officinalis L. is adaptable to different light environments.

  3. Impact of different culture media on hairy roots growth of Valeriana officinalis L.

    OpenAIRE

    Pakdin Parizi, Ali; Farsi, Mohammad; Nematzadeh, Ghorban-Ali; Amin MIRSHAMSI

    2015-01-01

    Transformed hairy root cultures of Valeriana officinalis were established by infection with Agrobacterium rhizogenes strain ATCC 15834. To determine the effect of different media on the growth of V. officinalis hairy roots, MS, B5 media (1.0X and 0.5X strength), N6 medium and a modified MS medium without phytohormones were used. In addition, different NH4+ to NO3- ratios in MS medium were studied. The effects of these treatments were evaluated after 21 days of culture in relation to hairy roo...

  4. Activation of the nuclear receptor PPARγ by metabolites isolated from sage (Salvia officinalis L.)

    DEFF Research Database (Denmark)

    Christensen, Kathrine Bisgaard; Jørgensen, Monica; Kotowska, Dorota Ewa

    2010-01-01

    . RESULTS: Eight diterpenes were isolated and identified including a new abietane diterpene being the epirosmanol ester of 12-O-methyl carnosic acid and 20-hydroxyferruginol, which was isolated from Salvia officinalis for the first time, as well as viridiflorol, oleanolic acid, and alpha-linolenic acid. 12......-O-methyl carnosic acid and alpha-linolenic acid were able to significantly activate PPARgamma whereas the remaining metabolites were either unable to activate PPARgamma or yielded insignificant activation. CONCLUSIONS: Selected metabolites from Salvia officinalis were able to activate PPARgamma...

  5. Antibacterial activities of Emblica officinalis and Coriandrum sativum against Gram negative urinary pathogens.

    Science.gov (United States)

    Saeed, Sabahat; Tariq, Perween

    2007-01-01

    Present investigation is focused on antibacterial potential of aqueous infusions and aqueous decoctions of Emblica officinalis (amla) and Coriandrum sativum (coriander) against 345 bacterial isolates belonging to 6 different genera of Gram negative bacterial population isolated from urine specimens by employing well diffusion technique. Aqueous infusion and decoction of Emblica officinalis exhibited potent antibacterial activity against Escherichia coli (270), Klebsiella pneumoniae (51), K. ozaenae (3), Proteus mirabilis (5), Pseudomonas aeruginosa (10), Salmonella typhi (1), S. paratyphi A (2), S. paratyphi B (1) and Serratia marcescens (2) but did not show any antibacterial activity against Gram negative urinary pathogens.

  6. Tissue-specific regulation of the LIM homeobox gene lin-11 during development of the Caenorhabditis elegans egg-laying system.

    Science.gov (United States)

    Gupta, Bhagwati P; Sternberg, Paul W

    2002-07-01

    The egg-laying system of Caenorhabditis elegans hermaphrodites requires development of the vulva and its precise connection with the uterus. This process is regulated by LET-23-mediated epidermal growth factor signaling and LIN-12-mediated lateral signaling pathways. Among the nuclear factors that act downstream of these pathways, the LIM homeobox gene lin-11 plays a major role. lin-11 mutant animals are egg-laying defective because of the abnormalities in vulval lineage and uterine seam-cell formation. However, the mechanisms providing specificity to lin-11 function are not understood. Here, we examine the regulation of lin-11 during development of the egg-laying system. Our results demonstrate that the tissue-specific expression of lin-11 is controlled by two distinct regulatory elements that function as independent modules and together specify a wild-type egg-laying system. A uterine pi lineage module depends on the LIN-12/Notch signaling, while a vulval module depends on the LIN-17-mediated Wnt signaling. These results provide a unique example of the tissue-specific regulation of a LIM homeobox gene by two evolutionarily conserved signaling pathways. Finally, we provide evidence that the regulation of lin-11 by LIN-12/Notch signaling is directly mediated by the Su(H)/CBF1 family member LAG-1.

  7. Structural requirements for the tissue-specific and tissue-general functions of the Caenorhabditis elegans epidermal growth factor LIN-3.

    Science.gov (United States)

    Liu, J; Tzou, P; Hill, R J; Sternberg, P W

    1999-01-01

    Caenorhabditis elegans lin-3 encodes a homolog of the epidermal growth factor (EGF) family of growth factors. LIN-3 is the inductive signal for hermaphrodite vulval differentiation, and it is required for animal viability, hermaphrodite fertility, and the specification of anterior cell fates in the male B cell lineage. We describe the cloning of a lin-3 homolog from C. briggsae, sequence comparison of C. elegans lin-3 with C. briggsae lin-3, and the determination of molecular lesions in alleles of C. elegans lin-3, including three new alleles. We also analyzed the severity of phenotypes caused by the new and existing alleles of lin-3. Correlation of mutant phenotypes and their molecular lesions, as well as sequence comparison between two species, reveal that the EGF motif and the N-terminal portion of the cytoplasmic domain are important for the functions of LIN-3 in all tissues, while the C-terminal portion of the cytoplasmic domain is involved in the tissue-specific functions of lin-3. We discuss how the structure of lin-3 contributes to its functions in multiple developmental processes. PMID:10545457

  8. Lin28a promotes self-renewal and proliferation of dairy goat spermatogonial stem cells (SSCs) through regulation of mTOR and PI3K/AKT

    Science.gov (United States)

    Ma, Fanglin; Zhou, Zhe; Li, Na; Zheng, Liming; Wu, Chongyang; Niu, Bowen; Tang, Furong; He, Xin; Li, Guangpeng; Hua, Jinlian

    2016-01-01

    Lin28a is a conserved RNA-binding protein that plays an important role in development, pluripotency, stemness maintenance, proliferation and self-renewal. Early studies showed that Lin28a serves as a marker of spermatogonial stem cells (SSCs) and promotes the proliferation capacity of mouse SSCs. However, there is little information about Lin28a in livestock SSCs. In this study, we cloned Capra hircus Lin28a CDS and found that it is evolutionarily conserved. Lin28a is widely expressed in different tissues of Capra hircus, but is expressed at a high level in the testis. Lin28a is specifically located in the cytoplasm of Capra hircus spermatogonial stem cells and may also be a marker of dairy goat spermatogonial stem cells. Lin28a promoted proliferation and maintained the self-renewal of GmGSCs-I-SB in vivo and in vitro. Lin28a-overexpressing GmGSCs-I-SB showed an enhanced proliferation rate, which might be due to increased PCNA expression. Moreover, Lin28a maintained the self-renewal of GmGSCs-I-SB by up-regulating the expression of OCT4, SOX2, GFRA1, PLZF and ETV5. Furthermore, we found that Lin28a may activate the AKT, ERK, and mTOR signaling pathways to promote the proliferation and maintain the self-renewal of GmGSCs-I-SB. PMID:27941834

  9. Ribosome-inactivating proteins from the seeds of Saponaria officinalis L. (soapwort), of Agrostemma githago L. (corn cockle) and of Asparagus officinalis L. (asparagus), and from the latex of Hura crepitans L. (sandbox tree).

    Science.gov (United States)

    Stirpe, F; Gasperi-Campani, A; Barbieri, L; Falasca, A; Abbondanza, A; Stevens, W A

    1983-12-15

    Ribosome-inactivating proteins, similar to those already known [Barbieri & Stirpe (1982) Cancer Surveys 1, 489-520] were purified from the seeds of Saponaria officinalis (two proteins), of Agrostemma githago (three proteins), and of Asparagus officinalis (three proteins), and from the latex of Hura crepitans (one protein). The yield ranged from 8 to 400 mg/100 g of starting material. All proteins have an Mr of approx. 30000 and an alkaline isoelectric point. Their sugar content varies from 0 (proteins from S. officinalis) to 40% (protein from H. crepitans). The ribosome-inactivating proteins inhibit protein synthesis by rabbit reticulocyte lysate, the ID50 (concentration giving 50% inhibition) ranging from 1 ng/ml (a protein from S. officinalis) to 18 ng/ml (a protein from A. githago). Those which were tested (the proteins from S. officinalis and from A. githago) also inhibit polymerization of phenylalanine by isolated ribosomes, acting in an apparently catalytic manner. The protein from H. crepitans inhibited protein synthesis by HeLa cells, with an ID50 of 4 micrograms/ml, whereas the proteins from S. officinalis and from A. githago had an ID50 of more than 50-100 micrograms/ml. The ribosome-inactivating proteins from S. officinalis and from A. githago reduced the number of local lesions by tobacco-mosaic virus in the leaves of Nicotiana glutinosa.

  10. Physical location of rice Gm-6,Pi-5(t) genes in O. officinalis with BAC-FISH

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    A fluorescence in situ hybridization (FISH) procedure was adoptedto physically map two rice BAC clones 24E21 and 4F22 linked to Gm-6 and Pi-5(t) in O. officinalis. FISH results showed that the two BAC clones were located at 4L. The percentage distance from the centromere to the hybridization sites was 72±2.62 for 24E21 and 54±5.43 for 4F22, the detection rates were 52.70% and 61.2%. The results obtained from the BAC and plasmid clones, RG214 and RZ565 of cultivated rice and O. officinalis were the same. This suggested that the markers, RG214 and RZ565 of cultivated rice and O. officinalis were in the same BAC clones. The homologous sequences of Gm-6 and Pi-5(t) in O. officinalis were positions that signals existed on the 4L. Many signals were observed when no Cot-1 DNA blocked. This also showed that repetitive sequences were some homolgous between cultivated rice and O. officinalis. The identification of chromosome 4 of O. officinalis is based on Jena et al. (1994). In our study, we discussed the possibility of physical map in O. officinalis with rice BAC clones.

  11. Effect of Ethanolic Extract of Emblica officinalis on Histopathology of Kidney and on Biochemical Parameters in Hyperlipidemic Albino Rats

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    Bheemshetty S. Patil

    2015-01-01

    Full Text Available Background: It has been reported that hyperlipidemia plays a central role in the development of atherosclerosis and oxidative stress. Embilica officinalis also known as Amla or Indian Gooseberry acts as antihyperlipidemic and antioxidant. Its active ingredients contains tannins, gallic acid and flavonoids. Aim & Objectives: It was aimed to evaluate the effect of ethanolic extract of Emblica officinalis on histopathology of kidney and on biochemical parameters in hyperlipidemic albino Wistar rats. Material and Methods: Extraction of dried fruits of Emblica officinalis was done by Soxhlet apparatus 0 using 99% ethanol at 60 C for 24 hours and also phytochemical analysis was done. Group I served as normal control. Group II was fed with isocaloric diet. Group III was fed with hyperlipidemic diet. Group IV was fed with isocaloric diet for 21 days + Embilica officinalis for 21 days. Group V was fed with hyperlipidemic diet for 21 days+ Embilica officinalis for 21 days. The dose of ethanolic extract of Emblica officinalis was taken as 100mg/kg body weight daily. Results: Percent body weight gain, kidney weight and nephro-somatic index significantly improved in hyperlipidemic rats treated with Emblica officinalis. There was a significant improvement in serum electrolyte and kidney markers. It was found that there were focal glomerular lesions with thickening of glomerulus in the kidneys of rats on hyperlipidemic diet and normal renal histology of rats on hyperlipidemic diet treated with Emblica officinalis. Conclusion: It can be concluded that Emblica officinalis may be a good, natural therapeutic agent against hyperlipidemic diet induced oxidative damage and nephrotoxicity.

  12. Evaluation of two natural extracts (Rosmarinus officinalis L. and Melissa officinalis L.) as antioxidants in cooked pork patties packed in MAP.

    Science.gov (United States)

    Lara, M S; Gutierrez, J I; Timón, M; Andrés, A I

    2011-07-01

    Two natural extracts, from rosemary (Rosmarinus officinalis L.; Nutrox) and lemon balm (Melissa officinalis L.; Meliox) showing a potential antioxidant activity, have been evaluated and compared with a synthetic antioxidant (Butylated hydroxytoluene; BHT) and a control batch. Pork meat patties were made by addition of the mentioned compounds, cooked and packed in modified atmosphere and stored under illumination for 6 days. A descriptive sensory analysis was also conducted. The patties with natural extracts showed higher a*-values (P<0.001) than control and BHT samples. Cooked pork hardness was the lowest for Meliox batch after 0 and 3 days of storage in comparison with the rest of the batches. Nutrox batch showed the lowest TBARS values and hexanal content (P<0.05) throughout the storage period. The batches with natural antioxidants produced the highest concentrations of free thiol groups after 0 and 3 days. Differences in sensory attributes among batches were not detected by the judge panel.

  13. MicroRNA-125b/Lin28 pathway contributes to the mesendodermal fate decision of embryonic stem cells.

    Science.gov (United States)

    Wang, Jia; Cao, Nan; Yuan, Min; Cui, Huijuan; Tang, Yuanjia; Qin, Lianju; Huang, Xinfang; Shen, Nan; Yang, Huang-Tian

    2012-06-10

    MicroRNAs (miRNAs) are important regulators of cell fate decisions, while the miRNAs and their targets in the regulation of stem cell differentiation are largely unidentified. Here we report novel functions of miR-125b/Lin28 axis in the regulation of mouse embryonic stem cell (mESC) lineage specification and cardiomyocyte differentiation. With a MicroRNA Array screen, we identified a number of miRNAs significantly changed during ESC differentiation, among which miR-125b showed a marked reduction during early differentiation. The abundantly expressed miR-125b in undifferentiated mESCs was dramatically downregulated to a level hardly detected during differentiation day 3 to 5, with a concomitant upregulation of Lin28. Ectopically expressing miR-125b did not alter characteristics of undifferentiated mESCs, whereas it impaired the endoderm and mesoderm development, but not the ectoderm, and inhibited cardiomyocyte formation. We further demonstrate that miR-125b targeted the 3'-untranslated region of Lin28 and reduced the abundance of Lin28 at both mRNA and protein levels. Moreover, phenotypes of miR-125b overexpressing cells were mimicked by downregulation of Lin28 and rescued by reintroduction of Lin28. In addition, the impaired cardiogenesis in miR-125b-introduced cells was greatly recovered when mimicking endoderm environment by cultivation with the condition medium from a visceral endoderm-like cell line, END-2. These results reveal that the miR-125b/Lin28 axis is an important regulator of early lineage specification and cardiomyocyte differentiation of ESCs.

  14. LIN7A depletion disrupts cerebral cortex development, contributing to intellectual disability in 12q21-deletion syndrome.

    Directory of Open Access Journals (Sweden)

    Ayumi Matsumoto

    Full Text Available Interstitial deletion of 12q21 has been reported in four cases, which share several common clinical features, including intellectual disability (ID, low-set ears, and minor cardiac abnormalities. Comparative genomic hybridization (CGH analysis using the Agilent Human Genome CGH 180K array was performed with the genomic DNA from a two-year-old Japanese boy with these symptoms, as well as hypoplasia of the corpus callosum. Consequently, a 14 Mb deletion at 12q21.2-q21.33 (nt. 77 203 574-91 264 613 bp, which includes 72 genes, was detected. Of these, we focused on LIN7A, which encodes a scaffold protein that is important for synaptic function, as a possible responsible gene for ID, and we analyzed its role in cerebral cortex development. Western blotting analyses revealed that Lin-7A is expressed on embryonic day (E 13.5, and gradually increases in the mouse brain during the embryonic stage. Biochemical fractionation resulted in the enrichment of Lin-7A in the presynaptic fraction. Suppression of Lin-7A expression by RNAi, using in utero electroporation on E14.5, delayed neuronal migration on postnatal day (P 2, and Lin-7A-deficient neurons remained in the lower zone of the cortical plate and the intermediate zone. In addition, when Lin-7A was silenced in cortical neurons in one hemisphere, axonal growth in the contralateral hemisphere was delayed; development of these neurons was disrupted such that one half did not extend into the contralateral hemisphere after leaving the corpus callosum. Taken together, LIN7A is a candidate gene responsible for 12q21-deletion syndrome, and abnormal neuronal migration and interhemispheric axon development may contribute to ID and corpus callosum hypoplasia, respectively.

  15. Effects of fluid extracts of Calendula on acute radiation dermatitis in rats%金盏花流浸膏对大鼠急性放射性皮肤损伤的影响

    Institute of Scientific and Technical Information of China (English)

    白雪; 刘美莲

    2013-01-01

    目的 观察金盏花流浸膏对大鼠急性放射性皮肤损伤的作用.方法 将36只Wistar雄性大鼠随机分为金盏花组、三乙醇胺组、对照组,每组12只.采用直线加速器6 MeV电子线照射,总照射剂量为5000cGy/20F,造成大白鼠臀部放射性皮肤损伤,每日于损伤部位涂药2次,评价损伤程度进行比较.结果 金盏花组和三乙醇胺组射野内皮肤不良反应均明显低于对照组(P均0.05).结论 金盏花流浸膏可明显减轻大鼠的急性放射性皮肤损伤,并促进其早期愈合.%Objective It is to observe the effects of fluid extracts of Calendula on acute radiation dermatitis in rats. Methods Thirty six male Wistar rats were randomly divided into three groups: Calendula group, trolamine group and control group, with 12 rats in each group. An electron beam with 6 MeV energy produced by a linear accelerator was used. The total irradiate dose was 5 000 cGy/20F. This caused acute radiation skin lesions to the gluteal area of rats. Calendula, trolamine were applied topically to the irradiated area twice a day after radiation. Acute skin reactions were evaluated and compared. Results The skin reactions in the Calendula and trolamine groups tended to be less marked than those in the control group ( P < 0.05 ). the Calendula group exhibited greater skin reaction than the trolamine group in early treatment stage, but they did not differ significantly at the end of the study ( P >0. 05 ). Conclusion Fluid extracts of Calendula can effectively reduce the acute radiation dermatitis damage and promote early healing.

  16. Processo de reparo em feridas de extração dentária em camundongos tratados com o complexo Symphytum officinale e Calendula officinallis

    Directory of Open Access Journals (Sweden)

    BALDUCCI-ROSLINDO Eleny

    1999-01-01

    Full Text Available Medicamentos homeopáticos como o Symphytum officinalle e a Calendula officinallis são dotados de propriedades anti-sépticas, antiinflamatória, cicatrizantes e também agem como promotores da consolidação de fraturas ósseas. Neste trabalho, uniram-se esses dois medicamentos similares em um complexo para verificar o seu efeito no reparo em feridas de extração dentária em camundongos. O complexo Symphytum officinalle e Calendula officinallis nas potências de 6CH e 3CH, respectivamente, foi ministrado por via oral ao grupo tratado durante 5 dias antes e após a extração do incisivo superior direito. No grupo controle, administraram-se 5ml de álcool etílico a 70% diluídos em 30 ml de soro fisiológico. Após a proservação, os animais foram sacrificados, a maxila direita separada da esquerda, fixada e processada para inclusão em parafina. Após a microtomia, os cortes obtidos foram corados pela H/E. A análise histológica mostrou que, tanto no grupo controle como no tratado, o alvéolo dentário estava preenchido por tecido de granulação e tecido ósseo neoformado, com graus variáveis de maturação, rico em osteócitos. No entanto, nos animais tratados, o processo de reparo em feridas após extração dentária do incisivo superior direito mostrou um avanço progressivo de neoformação óssea mais acentuado quando comparado ao grupo controle, em tempos equivalentes. Estes resultados enfatizam as propriedades biológicas do complexo Symphytum officinalle e Calendula officinallis e sua possível utilização como recurso terapêutico na Odontologia.

  17. Flowering biology and nectary structure of Melissa officinalis L.

    Directory of Open Access Journals (Sweden)

    Mirosława Chwil

    2012-12-01

    Full Text Available The present study on lemon balm (Melissa officinalis L. covered flowering biology, monitoring of pollinating insects and floral nectary structure. The micromorphology of epidermal cells of the nectary was investigated using scanning electron microscopy. The nectariferous tissues were observed using light microscopy based on semi-thin sections. Lemon balm flowered from the second decade of June until September. Buds opened from early morning hours until noon. Flowers lived for 24 hours, on the average. Their primary pollinator was the honey bee. The beginning of nectar secretion was found to be at the bud swell stage. The automorphic nectary forms a disc with four protrusions at the base of the nectary. Three smaller ones and one larger than the other ones were distinguished among them. No stomata were found on the lower protuberances, whereas on the highest part anomocytic stomata were present, the number of which was 15. The stomata exhibited different development stages and they were situated above other epidermal cells. In their outline, they were ellipsoidally shaped (18 × 23 µm and they had average-sized cuticular ledges. They produced a smooth cuticle and wax granules. In cross section, the nectary tissues were composed of a singlelayered epidermis and 9 - 11 layers of the nectary parenchyma. Their thickness was 198 µm. In longitudinal section, the height of the nectary was within a range of 354 - 404 µm. The epidermal cells produced thin outer cell walls. Some of them were completely filled with strongly stained cytoplasm, whereas others showed a high degree of vacuolisation. But the nectary parenchyma cells were marked by poorly stained cytoplasm, a large nucleus and vacuolisation of varying degree.

  18. The stardust family protein MPP7 forms a tripartite complex with LIN7 and DLG1 that regulates the stability and localization of DLG1 to cell junctions.

    Science.gov (United States)

    Bohl, Joanna; Brimer, Nicole; Lyons, Charles; Vande Pol, Scott B

    2007-03-30

    MPP7, a previously uncharacterized member of the p55 Stardust family of membrane-associated guanylate kinase (MAGUK) proteins, was found in a tripartite complex with DLG1 and LIN7A or LIN7C. MPP7 dimerizes with all three LIN7 family members (LIN7A, -B, and -C) through interaction of the single L27 domain of LIN7 with the carboxyl-terminal L27 domain of MPP7, thereby stabilizing both proteins. The dimer of MPP7 with LIN7A or LIN7C associates with DLG1 through an interaction requiring the amino-terminal L27 domain of MPP7. The amino-terminal L27 domain of MPP7 is not sufficient for interaction with DLG1 but interacts efficiently only if MPP7 is in a complex with LIN7A or -C. Thus the specificity of interaction of DLG1 with the LIN7-MPP7 complex is determined by L27 interactions with both MPP7 and LIN7. The tripartite complex forms in a ratio of 1:1:1 and localizes to epithelial adherens junctions in a manner dependent upon MPP7. Expression of MPP7 stabilizes DLG1 in an insoluble compartment. Expression of MPP7 deleted of the PDZ or Src homology 3 domain redistributes MPP7, DLG1, and LIN7 out of adherens junctions and into the soluble cytoplasmic fraction without changing the localization of E-cadherin. Thus, the stability and localization of DLG1 to cell-cell junctions are complex functions determined by the expression and association of particular Stardust family members together with particular LIN7 family members.

  19. Comparison of Antioxidant and Antimicrobial Properties for Ginkgo biloba and Rosemary (Rosmarinus officinalis L. from Egypt

    Directory of Open Access Journals (Sweden)

    Hossam S. EL-BELTAGI

    2013-05-01

    Full Text Available The widespread use of medicinal plants for health purposes has increased dramatically due to their great importance to the public health. In this study levels of phenolic, flavonoid contents of Ginkgo biloba and Rosmarinus officinalis from Egypt were determined. HPLC was used to identify and quantify the phenolic compounds in selected plants. The plant extracts were evaluated for their antioxidant activities using various antioxidant methodologies, (i scavenging of free radicals using 2, 2-diphenyl-1-picrylhydrazyl, (ii metal ion chelating capacity, and (iii scavenging of superoxide anion radical. The antimicrobial activity of both plant’s extracts were evaluated against a panel of microorganisms by using agar disc diffusion method. The total phenolic content (75.30 and 98.31 mg/g dry weight in G. biloba and R. officinalis, respectively was significantly (p<0.05 different. Among the identified phenolic compounds, quercetin, kaempferol and caffeic acid were the predominant phenolic compounds in Ginkgo biloba, whereas carnosic acid, rosmarinic acid, narinigen and hispidulin were the predominant phenolic compound in Rosmarinus officinalis leaves. The antioxidant activity increased with the concentration increase. The R. officinalis was more active than G. biloba extract against Gram-negative bacteria. This study reveals that the consumption of these plants would exert several beneficial effects by virtue of their antioxidant and antimicrobial activities.

  20. Evaluation of Emblica officinalis fruit powder as a growth promoter in commercial broiler chickens

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    A. P. Patel

    2016-02-01

    Full Text Available Aim: The present study was conducted to evaluate the dietary addition of Emblica officinalis (Amla fruit powder as a growth promoter in commercial broiler chickens. Materials and Methods: An experiment was conducted on 135 commercial broiler chicks (Ven-Cobb 400 strain divided into three groups with three replicates of 15 chicks each. Three treatment groups were as follows – T1: Basal diet as per BIS standards; T2: Basal diet supplemented with 0.4% of E. officinalis fruit powder; and T3: Basal diet supplemented with 0.8% of E. officinalis fruit powder. Results: The average body weights at the end of the 6th week were significantly higher (p<0.05 in groups T2 and T3 compared to group T1. Feed intake, feed conversion ratio and feed cost per kg live weight production were similar among the treatment groups. The net profit per bird was the highest in group T2 (Rs. 19.22/bird followed by group T3 (Rs. 17.86/bird and the lowest in group T1 (Rs. 14.61/bird. Conclusion: Based on the results of the present study, it was concluded that dietary addition of E. officinalis (Amla fruit powder had a positive effect on growth performance and net profit per bird in commercial broiler chickens.

  1. Larvicidal activity of essential extract of Rosmarinus officinalis against Culex quinquefasciatus.

    Science.gov (United States)

    Yu, Jing; Liu, Xiang-Yi; Yang, Bin; Wang, Jie; Zhang, Fu-Qiang; Feng, Zi-Liang; Wang, Chen-Zhu; Fan, Quan-Shui

    2013-03-01

    Constituents in rosemary (Rosmarinus officinalis) have been shown to have larvicidal activity against invertebrates. In order to explore the properties of crude extract of rosemary further, we studied the chemical composition and its activity against dichlorodiphenyltrichloroethane (DDT)-susceptible, DDT-resistant, and field strains of Culex quinquefasciatus larvae. The major components of R. officinalis were found to be eucalyptol and camphor, with relative percentages of 10.93% and 5.51%, respectively. Minor constituents included limonene, (+)-4-carene, isoborneol, 1-methyl-4-(1-methylethylidene)-cyclohexene, and pinene. The median lethal concentration (LC50) values of the essential oil of R. officinalis against DDT-susceptible, DDT-resistant, and field strains of larvae of Cx. quinquefasciatus were 30.6, 26.4, and 38.3 mg/liter, respectively. The single median lethal dose (LD50) in Kunming mice was 4752 mg/kg. Essential oils from R. officinalis may, therefore, provide an effective natural plant product for use in mosquito prevention and control.

  2. Neuroprotective and neurological properties of Melissa officinalis

    DEFF Research Database (Denmark)

    López, Víctor; Martín, Sara; Gómez-Serranillos, Maria Pilar;

    2009-01-01

    Melissa officinalis has traditionally been used due to its effects on nervous system. Both methanolic and aqueous extracts were tested for protective effects on the PC12 cell line, free radical scavenging properties and neurological activities (inhibition of MAO-A and acetylcholinesterase enzymes...

  3. orthodenticle/otx ortholog expression in the anterior brain and eyes of Sepia officinalis (Mollusca, Cephalopoda).

    Science.gov (United States)

    Buresi, Auxane; Baratte, Sébastien; Da Silva, Corinne; Bonnaud, Laure

    2012-01-01

    The origin of cerebral structures is a major issue in both developmental and evolutionary biology. Among Lophotrochozoans, cephalopods present both a derived nervous system and an original body plan, therefore they constitute a key model to study the evolution of nervous system and molecular processes that control the neural organization. We characterized a partial sequence of an ortholog of otx2 in Sepia officinalis embryos, a gene specific to the anterior nervous system and eye development. By in situ hybridization, we assessed the expression pattern of otx2 during S. officinalis organogenesis and we showed that otx is expressed (1) in the eyes, from early to late developmental stages as observed in other species (2) in the nervous system during late developmental stages. The otx ortholog does not appear to be required for the precocious emergence of the nervous ganglia in cephalopods and is later expressed only in the most anterior ganglia of the future brain. Finally, otx expression becomes restricted to localized part of the brain, where it could be involved in the functional specification of the central nervous system of S. officinalis. These results suggest a conserved involvement of otx in eye maturation and development of the anterior neural structures in S. officinalis.

  4. Dalmatian Sage (Salvia officinalis L.: A Review of Biochemical Contents, Medical Properties and Genetic Diversity

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    Martina Grdiša

    2015-11-01

    Full Text Available Dalmatian sage (Salvia officinalis L. represents one of the most significant medicinal autochthonous species in flora of eastern Adriatic coast and islands. It is evergreen outcrossing perennial subshrub with short woody stems that branch extensively and violet flowers. Apart from being native to Mediterranean karst of west Balkan and Apenine peninsula it is cultivated in numerous countries worldwide with Mediterranean and temperate continental climate. From the earliest times it has been used in traditional medicine in healing gingiva, mouth cavity and the sore throat, against bacterial and fungal infections, for wound treatment, memory enhancement, for treating common cold, against sweating, stomach inflammation, ulcer formation, etc. Its essential oil has also been used in preservation of food and as spice as it gives both specific aroma and promotes digestion of food. The essential oil is extremely complex mixture of different active ingredients; however, the thujones and camphor are the dominant compounds and are the parameter by which S. officinalis is distinguished from other Salvia species. The great variability of essential oil composition and yield has been detected depending on various factors such as genotype, environmental conditions, phonological stage, plant parts used for the extraction of essential oil and drying procedure. Molecular genetic analysis of S. officinalis is still limited and comprises the use of RAPD markers, AFLP and SSR markers in assessing mostly the genetic variability and structure of wild S. officinalis populations.

  5. Antioxidant activity and sensory assessment of a rosmarinic acid-enriched extract of Salvia officinalis

    Science.gov (United States)

    An extract of S. officinalis (garden sage) was prepared using supercritical carbon dioxide (SC-CO2) extraction, followed by a Soxhlet hot water extraction. The resulting extract was enriched in polyphenols, including rosmarinic acid (RA), which has shown promising health benefits in animals. The ext...

  6. Antimycotoxigenic characteristics of Rosmarinus officinalis and Trachyspermum copticum L. essential oils.

    Science.gov (United States)

    Rasooli, Iraj; Fakoor, Mohammad Hadi; Yadegarinia, Davod; Gachkar, Latif; Allameh, Abdolamir; Rezaei, Mohammad Bagher

    2008-02-29

    Aflatoxin B1 (AFB1) is a highly toxic and carcinogenic metabolite produced by Aspergillus species on food and agricultural commodities. Natural products may regulate the cellular effects of aflatoxins and evidence suggests that aromatic organic compounds of spices can control the production of aflatoxins. With a view to controlling aflatoxin production, the essential oils from Rosmarinus officinalis and Trachyspermum copticum L. were obtained by hydrodistillation. Antifungal activities of the oils were studied with special reference to the inhibition of Aspergillus parasiticus growth and aflatoxin production. Minimal inhibitory (MIC) and minimal fungicidal (MFC) concentrations of the oils were determined. T. copticum L. oil showed a stronger inhibitory effect than R. officinalis on the growth of A. parasiticus. Aflatoxin production was inhibited at 450 ppm of both oils with that of R. officinalis being stronger inhibitor. The oils were analyzed by GC and GC/MS. The major components of R. officinalis and T. copticum L. oils were Piperitone (23.65%), alpha-pinene (14.94%), Limonene (14.89%), 1,8-Cineole (7.43%) and Thymol (37.2%), P-Cymene (32.3%), gamma-Terpinene (27.3%) respectively. It is concluded that the essential oils could be safely used as preservative materials on some kinds of foods to protect them from toxigenic fungal infections.

  7. Hypnotic effect of Coriandrum sativum, Ziziphus jujuba, Lavandula angustifolia and Melissa officinalis extracts in mice.

    Science.gov (United States)

    Hajhashemi, Valiollah; Safaei, Azadeh

    2015-01-01

    The aim of the present study was to evaluate hypnotic effect of Coriandrum sativum, Ziziphus jujuba, Lavandula angustifolia and Melissa officinalis hydroalcoholic extracts in mice to select the most effective ones for a combination formula. Three doses of the extracts (250, 500 and 1000 mg/kg of C. sativum and Z. jujuba and 200, 400 and 800 mg/kg of L. angustifolia and M. officinalis) were orally administered to male Swiss mice (20-25 g) and one hour later pentobarbital (50 mg/kg, i.p.) was injected to induce sleep. Onset of sleep and its duration were measured and compared. Control animals and reference group received vehicle (10 ml/kg, p.o.) and diazepam (3 mg/kg, i.p.), respectively. C. sativum and Z. jujuba failed to change sleep parameters. L. angustifolia at doses of 200, 400 and 800 mg/kg shortened sleep onset by 7.6%, 50% and 51.5% and prolonged sleep duration by 9.9%, 43.1% and 80.2%, respectively. Compared with control group the same doses of M. officinalis also decreased sleep onset by 24.7%, 27.5% and 51.2% and prolonged sleep duration by 37.9%, 68.7% and 131.7% respectively. Combinations of L. angustifolia and M. officinalis extracts showed additive effect and it is suggested that a preparation containing both extracts may be useful for insomnia.

  8. Synergistic Effect of Selenium Addition and Water Stress on Melilotus officinalis L. Mineral Content

    Directory of Open Access Journals (Sweden)

    Panagiota KOSTOPOULOU

    2015-12-01

    Full Text Available The objective of this study was to examine the combined effects of selenium (Se enrichment and water stress on the accumulation of available macro- and micronutrients in Melilotus officinalis L. aerial parts. Plants of M. officinalis were subjected to three levels of Se addition (0, 1 and 3 mg Se L-1 water and to two water treatments: a full irrigation and b limited irrigation (water stress. The above ground biomass (stems and leaves was analyzed for Se, potassium (K, sodium (Na, magnesium (Mg, iron (Fe, copper (Cu, calcium (Ca, manganese (Mn and zinc (Zn. Se addition differentially affected the K, Mg and Ca content of M. officinalis aerial parts, while it led to the reduction of the micronutrients Cu, Fe and Mn. Water stress resulted in the increase of K, Na, Mg, Ca and Cu, and to the decrease of the Fe, Zn and Mn content. An interaction between selenium addition and water treatment was more notable for Ca and Mg, which decreased under water stress at low Se level and for Zn and Cu, which increased under water stress at high Se level. According to our findings, Se-induced increased accumulation of some inorganic ions in the aerial parts of this species under water stress conditions could serve as a means to alleviate the adverse impact of water deficit on important metabolic processes, enhancing M. officinalis tolerance to water stress.

  9. LIN28B polymorphisms are associated with central precocious puberty and early puberty in girls

    Directory of Open Access Journals (Sweden)

    Sung Won Park

    2012-10-01

    Full Text Available &lt;B&gt;Purpose:&lt;/B&gt; Single-nucleotide polymorphism (SNP markers within &lt;i&gt;LIN28B&lt;/i&gt; have been reported to be related to the timing of pubertal growth. However, no study has investigated the frequency of genetic markers in girls with precocious puberty (PP or early puberty (EP. This study aimed to determine the frequency of putative genetic markers in girls with PP or EP. &lt;B&gt;Methods:&lt;/B&gt; Genomic DNAs were obtained from 77 and 109 girls that fulfilled the criteria for PP and EP, respectively. The controls in this study were 144 healthy volunteers between 20 and 30 years of age. The haplotypes were reconstructed using 11 SNPs of &lt;i&gt;LIN28B&lt;/i&gt;, and haplotype association analysis was performed. The haplotype frequencies were compared. Differences in the clinical and laboratory parameters were analyzed according to the haplotype dosage. &lt;B&gt;Results:&lt;/B&gt; Eleven SNPs in &lt;i&gt;LIN28B&lt;/i&gt; were all located in a block that was in linkage disequilibrium. The haplotype could be reconstructed using 2 representative SNPs, rs4946651 and rs369065. The AC haplotype was less frequently observed in the PP group than in the controls (0.069 vs. 0.144, &lt;I&gt;P&lt;/I&gt;=0.010. The trend that girls with non-AC haplotypes tended to have earlier puberty onset (&lt;I&gt;P&lt;/I&gt;=0.037 was illustrated even in the EP+PP patient group by Kaplan-Meier analysis. &lt;B&gt;Conclusion:&lt;/B&gt; The results of the present study showed that non-AC haplotypes of &lt;i&gt;LIN28B&lt;/i&gt;had a significant association with PP in girls.

  10. Décoloration d’une huile végétale (huile de lin)

    OpenAIRE

    TAHRAOUI, sarah

    2014-01-01

    aprés anlyses chimique et phsico-chimique de cinq terres décolorante différentes (tonsil EX 096 otimum (allemagne), T.D de maghnia (algérie) , t.d de turquie et T.D d'indonésie,nous avons effectué la décoloration d'une huile de lin par les cinq terres activées en faisant varier plusieurs paéramétre comme le temps d'agitation,la température de l'huile,le type et le pourcentage ed terre utilisée.

  11. [Lin Xue-Jian's experience on treatment of a part of cerebral diseases with scalp acupuncture].

    Science.gov (United States)

    Wang, Hai-Li; Wu, Jiu-Wei

    2005-10-01

    LIN Xue-Jian adopts Chinese traditional acupuncture and moxibustion manipulation methods to stimulate the special area of scalp to treat a part of brain-derived diseases, such as infantile cerebral palsy, nerve deafness, cerebellar ataxia, lacunar cerebral infarction, senile dementia, Parkinson's disease, anxiety, insomnia and central constipation, and so on. Scalp acupuncture can improve ability of blood and oxygen supply for general blood vessels; stimulation of corresponding acupoint area according to symptoms and signs can control condition of disease; and can repair, activate and regenerate the injured, dormancy and aging neurons, so as to dredge nerve network in the brain, hence better therapeutic effect.

  12. A new approach to control the modified LinVerter for high frequency applications

    DEFF Research Database (Denmark)

    Farhang, Peyman; Mátéfi-Tempfli, Stefan

    2016-01-01

    control perspective, a novel control approach based on bidirectional interface between LTspice and MATLAB is created. In this case, the circuit is modeled in LTspice environment and a Chaos Optimization Algorithm (COA) is coded in MATLAB in order to find out the optimal solution in control process....... In fact, this new approach combines the advantages of LTspice for simulation of different circuit configurations using actual component obtained from manufactures' models with advanced intelligent techniques capabilities from COA in MATLAB. First, the performance of proposed multi-device LinVerter along...

  13. The Study on Lin Yutang’s Translation from the Perspective of Aesthetics

    Institute of Scientific and Technical Information of China (English)

    瞿宁

    2013-01-01

    Since the main purposes of translation lies in expressing to the target readers both the message and the aesthetic value of the source text and making the readers ideologically inspired and aesthetically entertained, Lin Yutang conclusively sum up that the aesthetic criteria in translation should be faithfulness, expressiveness and beauty throughout his translation practices. This thesis mainly focus on the aesthetic criteria of Lin’s translation as well as the aesthetic reproduction in his translation which illustrates the unity reached in Lin’s translation in terms of both the aesthetic features and the loyalty to the meaning.

  14. In vitro effects of Salvia officinalis L. essential oil on Candida albicans

    Institute of Scientific and Technical Information of China (English)

    Tularat Sookto; Theerathavaj Srithavaj; Sroisiri Thaweboon; Boonyanit Thaweboon; Binit Shrestha

    2013-01-01

    Objective: To determine the anticandidal activities of Salvia officinalis L. (S. officinalis) essential oil against Candida albicans (C. albicans) and the inhibitory effects on the adhesion of C. albicans to polymethyl methacrylate (PMMA) resin surface. Methods: Disc diffusion method was first used to test the anticandidal activities of the S. officinalis L. essential oil against the reference strain (ATCC 90028) and 2 clinical strains of C. albicans. Then the minimal inhibitory concentration (MIC) and minimal lethal concentration (MLC) were determined by modified membrane method. The adhesion of C. albicans to PMMA resin surface was assessed after immersion with S. officinalis L. essential oil at various concentrations of 1×MIC, 0.5×MIC and 0.25×MIC at room temperature for 30 min. One-way ANOVA was used to compare the Candida cell adhesion with the pretreatment agents and Tukey’s test was used for multiple comparisons. Results: S. officinalis L. essential oil exhibited anticandidal activity against all strains of C. albicans with inhibition zone ranging from 40.5 mm to 19.5 mm. The MIC and MLC of the oil were determined as 2.780 g/L against all test strains. According to the effects on C. albicans adhesion to PMMA resin surface, it was found that immersion in the essential oil at concentrations of 1×MIC (2.780 g/L), 0.5×MIC (1.390 g/L) and 0.25×MIC (0.695 g/L) for 30 min significantly reduced the adhesion of all 3 test strains to PMMA resin surface in a dose dependent manner (P<0.05). Conclusions: S. officinalis L. essential oil exhibited anticandidal activities against C. albicans and had inhibitory effects on the adhesion of the cells to PMMA resin surface. With further testing and development, S. officinalis essential oil may be used as an antifungal denture cleanser to prevent candidal adhesion and thus reduce the risk of candida-associated denture stomatitis.

  15. Genome packaging in EL and Lin68, two giant phiKZ-like bacteriophages of P. aeruginosa

    Energy Technology Data Exchange (ETDEWEB)

    Sokolova, O.S., E-mail: sokolova@mail.bio.msu.ru [M.V. Lomonosov Moscow State University, Moscow (Russian Federation); A.V. Shoubnikov Institute of Crystallography RAS, Moscow (Russian Federation); Shaburova, O.V. [I.I. Mechnikov Research Institute of Vaccines and Sera, RAMS, Moscow (Russian Federation); Pechnikova, E.V. [A.V. Shoubnikov Institute of Crystallography RAS, Moscow (Russian Federation); Shaytan, A.K. [M.V. Lomonosov Moscow State University, Moscow (Russian Federation); Krylov, S.V. [I.I. Mechnikov Research Institute of Vaccines and Sera, RAMS, Moscow (Russian Federation); Kiselev, N.A. [A.V. Shoubnikov Institute of Crystallography RAS, Moscow (Russian Federation); Krylov, V.N. [I.I. Mechnikov Research Institute of Vaccines and Sera, RAMS, Moscow (Russian Federation)

    2014-11-15

    A unique feature of the Pseudomonas aeruginosa giant phage phiKZ is its way of genome packaging onto a spool-like protein structure, the inner body. Until recently, no similar structures have been detected in other phages. We have studied DNA packaging in P. aeruginosa phages EL and Lin68 using cryo-electron microscopy and revealed the presence of inner bodies. The shape and positioning of the inner body and the density of the DNA packaging in EL are different from those found in phiKZ and Lin68. This internal organization explains how the shorter EL genome is packed into a large EL capsid, which has the same external dimensions as the capsids of phiKZ and Lin68. The similarity in the structural organization in EL and other phiKZ-like phages indicates that EL is phylogenetically related to other phiKZ-like phages, and, despite the lack of detectable DNA homology, EL, phiKZ, and Lin68 descend from a common ancestor. - Highlights: • We performed a comparative structural study of giant P. aeruginosa phages: EL, Lin68 and phiKZ. • We revealed that the inner body is a common feature in giant phages. • The phage genome size correlates with the overall dimensions of the inner body.

  16. De Novo Transcriptome Sequencing of Oryza officinalis Wall ex Watt to Identify Disease-Resistance Genes

    Directory of Open Access Journals (Sweden)

    Bin He

    2015-12-01

    Full Text Available Oryza officinalis Wall ex Watt is one of the most important wild relatives of cultivated rice and exhibits high resistance to many diseases. It has been used as a source of genes for introgression into cultivated rice. However, there are limited genomic resources and little genetic information publicly reported for this species. To better understand the pathways and factors involved in disease resistance and accelerating the process of rice breeding, we carried out a de novo transcriptome sequencing of O. officinalis. In this research, 137,229 contigs were obtained ranging from 200 to 19,214 bp with an N50 of 2331 bp through de novo assembly of leaves, stems and roots in O. officinalis using an Illumina HiSeq 2000 platform. Based on sequence similarity searches against a non-redundant protein database, a total of 88,249 contigs were annotated with gene descriptions and 75,589 transcripts were further assigned to GO terms. Candidate genes for plant–pathogen interaction and plant hormones regulation pathways involved in disease-resistance were identified. Further analyses of gene expression profiles showed that the majority of genes related to disease resistance were all expressed in the three tissues. In addition, there are two kinds of rice bacterial blight-resistant genes in O. officinalis, including two Xa1 genes and three Xa26 genes. All 2 Xa1 genes showed the highest expression level in stem, whereas one of Xa26 was expressed dominantly in leaf and other 2 Xa26 genes displayed low expression level in all three tissues. This transcriptomic database provides an opportunity for identifying the genes involved in disease-resistance and will provide a basis for studying functional genomics of O. officinalis and genetic improvement of cultivated rice in the future.

  17. Study on the Growth-development Law and Characteristics of Calendula%金盏花生长发育规律及特征特性研究

    Institute of Scientific and Technical Information of China (English)

    周彦芳; 刘强; 杨宪忠; 张秀华

    2009-01-01

    [目的]了解金盏花的养分需求规律及生产潜力.[方法]通过大田试验研究金盏花干物质积累和分布的动态变化规律,并考察其生产潜力等特征特性.[结果]植株的最大干物质积累速率出现在7月下旬,根的最大干物质积累速率出现在6月26日,茎枝的最大干物质积累速率出现在6月中、下旬,鲜花的最大干物质积累速率为2.4 g/d,出现在8月15日.苗期的叶片干物质积累量在植株干物质积累量中的比例高于60%,开花后根系的比例最大,7月下旬茎枝的比例最大.金盏花的有效分枝可达100个,鲜花产量高于45 t/hm2,单花鲜重为7.5~20.0 g,株高为85~100 cm,鲜花的叶黄素含量为12.5~16.5 g/kg.[结论]金盏花有2个旺长期,分别在现蕾期和初花-盛花期,其干物质积累呈"慢-快-慢-快-慢"的变化规律.%[Objective]The aim of the study was to learn about the nutrient requirement characteristics and productive potential of calendula. [Method]The dynamic variation rule of dry-matter accumulation and distribution in calendula was studied and its characteristics including productive potential were investigated through field experiment. [Result]The highest dry-matter accumulation rate in plant occurred in late Jul., that in root occurred on Jun. 26th, that in stems and branches occurred in middle and late Jun. and that in fresh flower was 2.4 g/d and occurred on Aug. 15th. The proportion of dry-matter accumulation amount in leaf in that of the whole plant was higher than 60% in the seedling stage, the proportion in root was biggest after blooming and the proportion in stems and branches was biggest in late Jul. The calendula could have 100 effective branches at most, its fresh flower yield was higher than 45 t/hm2, its fresh weight of single flower was 7.5-20.0 g, its plant height was 85-100 cm and its lutein content in fresh flower was 12.5-16.5 g/kg. [Conclusion]Calendula had 2 vigorous growth periods and they occurred

  18. Anxiolytic and antidepressant-like effects of Melissa officinalis (lemon balm extract in rats: Influence of administration and gender

    Directory of Open Access Journals (Sweden)

    Adefunmilayo E Taiwo

    2012-01-01

    Conclusion: The potential psychoactive properties of M. officinalis may provide a unique pharmacological alternative for certain psychiatric disorders; however, the efficacy appears to be dependent on both gender and administration length.

  19. Chemical composition and biological activity of essential oils of Dracocephalum heterophyllum and Hyssopus officinalis from Western Himalaya

    Science.gov (United States)

    The essential oils of two representatives of the Lamiaceae-family, Dracocephalum heterophyllum Benth. and Hyssopus officinalis L., are described for their antifungal, antibacterial and larvicidal as well as biting deterrent activities. Additionally, the essential oils’ chemical compositions, analyze...

  20. Characterization of two genes for the biosynthesis of abietane-type diterpenes in rosemary (Rosmarinus officinalis) glandular trichomes

    NARCIS (Netherlands)

    Brückner, K.; Bozic, D.; Manzano, D.; Papaefthimiou, D.; Pateraki, I.; Scheler, U.; Ferrer, A.; Vos, de R.C.H.; Kanellis, A.K.; Tissier, A.

    2014-01-01

    Rosemary (Rosmarinus officinalis) produces the phenolic diterpenes carnosic acid and carnosol, which, in addition to their general antioxidant activities, have recently been suggested as potential ingredients for the prevention and treatment of neurodegenerative diseases. Little is known about the b

  1. zePPeLIN: Distributed Path Planning Using an Overhead Camera Network

    Directory of Open Access Journals (Sweden)

    Andreagiovanni Reina

    2014-08-01

    Full Text Available We introduce zePPeLIN, a distributed system designed to address the challenges of path planning in large, cluttered, dynamic environments. The objective is to define a sequence of instructions to precisely move a ground object (e.g., a mobile robot from an initial to a final configuration in an environment. zePPeLIN is based on a set of wirelessly networked overhead cameras. While each camera only covers a limited environment portion, the camera set fully covers the environment through the union of its fields of view. Path planning is performed in a fully distributed and cooperative way, based on potential diffusion over local Voronoi skeletons and local message exchanging. Additionally, the control of the moving object is fully distributed: it receives movement instructions from each camera when it enters that camera’s field of view. The overall task is made particularly challenging by intrinsic errors in the overlap in cameras’ fields of view. We study the performance of the system as a function of these errors, as well as its scalability for the size and density of the camera network. We also propose a few heuristics to improve performance and computational and communication efficiency. The reported results include both extensive simulation experiments and validation using a real camera network planning for a two-robot system.

  2. 林则徐与科举%Lin Zexu And Imperial Examination

    Institute of Scientific and Technical Information of China (English)

    张媛媛

    2012-01-01

    Lin Zexu plays a decisive role in modern history, and in the imperial examinations, he also occupies a space. As the examination official, he reformed some of the disadvantages in imperial examination, and made some achievements. By the study of the relationship between Lin Zexu and the imperial examination , we can get some enlightenment.%林则徐是中国近代史上举足轻重的人物,在科举史上同样占有一席之地。在家庭以及社会的影响下林则徐走上了科举之路,并最终金榜题名。作为科考试官他公正衡文、选拔真才,并在江南乡试期间改革科场弊端,取得了一定成效。林则徐提倡“经世致用”,并且贯彻于书院教育和科举考试之中,以期士子能关注社会现实,解决社会实际问题。通过考察林则徐与科举的关系,试图从中得到一些启示。

  3. The Overviews on the Research on Lin Gengbai%林庚白研究综述

    Institute of Scientific and Technical Information of China (English)

    闵军

    2011-01-01

    林庚白是民国期间写作旧体诗词的重要诗人。我们大致可以上世纪90年代为界,把林庚白研究分为前后两个时期,前一时期为林庚白研究的奠基期,后一时期为林庚白研究的拓展期。其研究主要从生平事迹的整理和作品研究两个方面展开。我们要继续做好林庚白的作品辑佚、生平事迹、作品研究等工作。%L in Geng -bai is an important poet who writes classical poet during the Republican China. Roughly bounded by the 1990s, the study on Lin Geng - bai can be divided into two periods. The previous period for the study is the foundation period, and the later is the expansion period. The study mainly expands in two ways, the collection of his life biography and works study. We should continue to do the research on Lin Geng-bai, such as his works collection, biography, and works study.

  4. A Novel Method to Increase LinLog CMOS Sensors’ Performance in High Dynamic Range Scenarios

    Directory of Open Access Journals (Sweden)

    Andrés Iborra

    2011-08-01

    Full Text Available Images from high dynamic range (HDR scenes must be obtained with minimum loss of information. For this purpose it is necessary to take full advantage of the quantification levels provided by the CCD/CMOS image sensor. LinLog CMOS sensors satisfy the above demand by offering an adjustable response curve that combines linear and logarithmic responses. This paper presents a novel method to quickly adjust the parameters that control the response curve of a LinLog CMOS image sensor. We propose to use an Adaptive Proportional-Integral-Derivative controller to adjust the exposure time of the sensor, together with control algorithms based on the saturation level and the entropy of the images. With this method the sensor’s maximum dynamic range (120 dB can be used to acquire good quality images from HDR scenes with fast, automatic adaptation to scene conditions. Adaptation to a new scene is rapid, with a sensor response adjustment of less than eight frames when working in real time video mode. At least 67% of the scene entropy can be retained with this method.

  5. A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens

    Science.gov (United States)

    Li, Chen; Sako, Yusuke; Imai, Akihiro; Nishiyama, Tomoaki; Thompson, Kari; Kubo, Minoru; Hiwatashi, Yuji; Kabeya, Yukiko; Karlson, Dale; Wu, Shu-Hsing; Ishikawa, Masaki; Murata, Takashi; Benfey, Philip N.; Sato, Yoshikatsu; Tamada, Yosuke; Hasebe, Mitsuyasu

    2017-01-01

    Both land plants and metazoa have the capacity to reprogram differentiated cells to stem cells. Here we show that the moss Physcomitrella patens Cold-Shock Domain Protein 1 (PpCSP1) regulates reprogramming of differentiated leaf cells to chloronema apical stem cells and shares conserved domains with the induced pluripotent stem cell factor Lin28 in mammals. PpCSP1 accumulates in the reprogramming cells and is maintained throughout the reprogramming process and in the resultant stem cells. Expression of PpCSP1 is negatively regulated by its 3′-untranslated region (3′-UTR). Removal of the 3′-UTR stabilizes PpCSP1 transcripts, results in accumulation of PpCSP1 protein and enhances reprogramming. A quadruple deletion mutant of PpCSP1 and three closely related PpCSP genes exhibits attenuated reprogramming indicating that the PpCSP genes function redundantly in cellular reprogramming. Taken together, these data demonstrate a positive role of PpCSP1 in reprogramming, which is similar to the function of mammalian Lin28. PMID:28128346

  6. Genetic variation in LIN28B is associated with the timing of puberty.

    Science.gov (United States)

    Ong, Ken K; Elks, Cathy E; Li, Shengxu; Zhao, Jing Hua; Luan, Jian'an; Andersen, Lars B; Bingham, Sheila A; Brage, Soren; Smith, George Davey; Ekelund, Ulf; Gillson, Christopher J; Glaser, Beate; Golding, Jean; Hardy, Rebecca; Khaw, Kay-Tee; Kuh, Diana; Luben, Robert; Marcus, Michele; McGeehin, Michael A; Ness, Andrew R; Northstone, Kate; Ring, Susan M; Rubin, Carol; Sims, Matthew A; Song, Kijoung; Strachan, David P; Vollenweider, Peter; Waeber, Gerard; Waterworth, Dawn M; Wong, Andrew; Deloukas, Panagiotis; Barroso, Inês; Mooser, Vincent; Loos, Ruth J; Wareham, Nicholas J

    2009-06-01

    The timing of puberty is highly variable. We carried out a genome-wide association study for age at menarche in 4,714 women and report an association in LIN28B on chromosome 6 (rs314276, minor allele frequency (MAF) = 0.33, P = 1.5 × 10(-8)). In independent replication studies in 16,373 women, each major allele was associated with 0.12 years earlier menarche (95% CI = 0.08-0.16; P = 2.8 × 10(-10); combined P = 3.6 × 10(-16)). This allele was also associated with earlier breast development in girls (P = 0.001; N = 4,271); earlier voice breaking (P = 0.006, N = 1,026) and more advanced pubic hair development in boys (P = 0.01; N = 4,588); a faster tempo of height growth in girls (P = 0.00008; N = 4,271) and boys (P = 0.03; N = 4,588); and shorter adult height in women (P = 3.6 × 10(-7); N = 17,274) and men (P = 0.006; N = 9,840) in keeping with earlier growth cessation. These studies identify variation in LIN28B, a potent and specific regulator of microRNA processing, as the first genetic determinant regulating the timing of human pubertal growth and development.

  7. XSHOOTER spectroscopy of the enigmatic planetary nebula Lin49 in the Small Magellanic Cloud

    CERN Document Server

    Otsuka, Masaaki; Leal-Ferreira, M L; Aleman, I; Bernard-Salas, J; Cami, J; Ochsendorf, B; Peeters, E; Scicluna, P

    2016-01-01

    We performed a detailed spectroscopic analysis of the fullerene C60-containing planetary nebula (PN) Lin49 in the Small Magellanic Cloud using XSHOOTER at the ESO VLT and the Spitzer/IRS instruments. We derived nebular abundances for nine elements. We used TLUSTY to derive photospheric parameters for the central star. Lin49 is C-rich and metal-deficient PN (Z~0.0006). The nebular abundances are in good agreement with Asymptotic Giant Branch nucleosynthesis models for stars with initial mass 1.25 Msun and metallicity Z = 0.001. Using the TLUSTY synthetic spectrum of the central star to define the heating and ionising source, we constructed the photoionisation model with CLOUDY that matches the observed spectral energy distribution (SED) and the line fluxes in the UV to far-IR wavelength ranges simultaneously. We could not fit the ~1-5 um SED using a model with 0.005-0.1 um-sized graphite grains and a constant hydrogen density shell owing to the prominent near-IR excess, while at other wavelengths the model fit...

  8. Wolbachia induces male-specific mortality in the mosquito Culex pipiens (LIN strain.

    Directory of Open Access Journals (Sweden)

    Jason L Rasgon

    Full Text Available BACKGROUND: Wolbachia are maternally inherited endosymbionts that infect a diverse range of invertebrates, including insects, arachnids, crustaceans and filarial nematodes. Wolbachia are responsible for causing diverse reproductive alterations in their invertebrate hosts that maximize their transmission to the next generation. Evolutionary theory suggests that due to maternal inheritance, Wolbachia should evolve toward mutualism in infected females, but strict maternal inheritance means there is no corresponding force to select for Wolbachia strains that are mutualistic in males. METHODOLOGY/PRINCIPAL FINDINGS: Using cohort life-table analysis, we demonstrate that in the mosquito Culex pipiens (LIN strain, Wolbachia-infected females show no fitness costs due to infection. However, Wolbachia induces up to a 30% reduction in male lifespan. CONCLUSIONS/SIGNIFICANCE: These results indicate that the Wolbachia infection of the Culex pipiens LIN strain is virulent in a sex-specific manner. Under laboratory situations where mosquitoes generally mate at young ages, Wolbachia strains that reduce male survival could evolve by drift because increased mortality in older males is not a significant selective force.

  9. Evaluation of the antibacterial potential of Petroselinum crispum and Rosmarinus officinalis against bacteria that cause urinary tract infections

    OpenAIRE

    Fernanda Villas Boas Petrolini; Rodrigo Lucarini; Maria Gorete Mendes de Souza; Regina Helena Pires; Wilson Roberto Cunha; Carlos Henrique Gomes Martins

    2013-01-01

    In this study we evaluated the antibacterial activity of the crude hydroalcoholic extracts, fractions, and compounds of two plant species, namely Rosmarinus officinalis and Petroselinum crispum, against the bacteria that cause urinary tract infection. The microdilution method was used for determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The crude hydroalcoholic extract of R. officinalis displayed in vitro activity against Gram-positive ...

  10. Anticonvulsant and analgesic activities of crude extract and its fractions of the defensive secretion from the Mediterranean sponge, Spongia officinalis.

    OpenAIRE

    2012-01-01

    Abstract This study progresses in the direction of identifying component(s) from the Mediterranean sponge, Spongia officinalis with anticonvulsant and analgesic activities. We investigated the efficacy of crude extract and its semi-purified fractions (F1-F3) of the defensive secretion from Spongia officinalis for their in vivo anticonvulsant activity using the pentylenetetrazole (PTZ) seizure model and analgesic activity using the writhing test in mice. Among the series the crude extract exhi...

  11. Total Content of Polyphenols and Antioxidant Activity of Different Melliferous Plants

    OpenAIRE

    2016-01-01

    In this study polyphenols content and antioxidant activity of melliferous plants for the following: mint (Mentha pulegium), burdock (Arctium lappa), comfrey (Symphytum officinale), plantain (Pantago lanceolata), thyme (Thymus vulgaris), sage (Salvia officinalis), marigold (Calendula officinalis), small marshmallow (Althaea officinalis), echinacea (Echinaceea angustifolia) and black popular (Populus nigra) were investigated, using two different extraction methods. High content of polyp...

  12. Esterco bovino e biofertilizante no cultivo de erva-cidreira-verdadeira (Melissa officinalis L. Cattle manure and biofertilizer on the cultivation of lemon balm (Melissa officinalis L.

    Directory of Open Access Journals (Sweden)

    M.F. Santos

    2009-01-01

    Full Text Available A importância fitoterápica e, consequentemente, econômica da Melissa officinalis tem contribuído para expansão do cultivo. O óleo essencial das folhas é formado por constituintes químicos que podem ser largamente empregados na indústria farmacêutica por possuírem atividades antioxidativa, antivirótica e sedativa. Assim sendo, objetivou-se no presente trabalho avaliar o efeito de combinações de doses de adubação orgânica com biofertilizante comercial e esterco bovino no cultivo de Melissa officinalis. Os tratamentos foram constituídos de quatro doses de biofertilizante orgânico (Vitassolo® e esterco bovino, sendo estes de 0, 30.000, 60.000 e 90.000 L ha-1. Os tratamentos foram arranjados no delineamento experimental blocos casualizados no fatorial 4x4, com 3 repetições. As características avaliadas foram altura de planta, rendimento de folha, teor e rendimento de óleo essencial. As maiores doses de esterco bovino resultaram em um maior desenvolvimento das plantas, em relação às mesmas doses do biofertilizante.The phytotherapic and economic importance of Melissa officinalis has contributed to its cultivation expansion. The essential oil of leaves is composed of chemical constituents, which can be largely employed in the pharmaceutical industry due to their antioxidant, antivirotic and sedative activities. Thus, the aim of the present work was to evaluate the effect of level combination of organic fertilization with commercial biofertilizer and cattle manure on Melissa officinalis cultivation. Treatments constituted of four levels of organic biofertilizer (Vitassolo® and cattle manure at 0; 30,000; 60,000 and 90,000 L ha-1. Experimental design was in randomized blocks, in split plot 4X4 factorial arrangement, with three replicates. The characteristics evaluated were plant height, leaf yield, essential oil content and yield. The highest cattle manure levels resulted in higher plant development, compared with the same

  13. Reduction of EMC Emissions in Mixed Signal Integrated Circuits with Embedded LIN Driver

    Directory of Open Access Journals (Sweden)

    P. Hartl

    2016-06-01

    Full Text Available This paper describes several methods for reduction of electromagnetic emissions (EME of mixed signal integrated circuits (IC. The focus is on the impact that a LIN bus communication block has on a complex IC which contains analog blocks, noisy digital block, micro-core (µC and several types of memories. It is used in an automotive environment, where EMC emission reduction is one of the key success factors. Several proposed methods for EME reduction are described and implemented on three test chips. These methods include current consumption reduction, internal on-chip decoupling, ground separation and different linear voltage regulator topologies. Measurement results of several fabricated test chips are shown and discussed.

  14. QUANTUM INSPIRED PARTICLE SWARM COMBINED WITH LIN-KERNIGHAN-HELSGAUN METHOD TO THE TRAVELING SALESMAN PROBLEM

    Directory of Open Access Journals (Sweden)

    Bruno Avila Leal de Meirelles Herrera

    2015-12-01

    Full Text Available ABSTRACT The Traveling Salesman Problem (TSP is one of the most well-known and studied problems of Operations Research field, more specifically, in the Combinatorial Optimization field. As the TSP is a NP (Non-Deterministic Polynomial time-hard problem, there are several heuristic methods which have been proposed for the past decades in the attempt to solve it the best possible way. The aim of this work is to introduce and to evaluate the performance of some approaches for achieving optimal solution considering some symmetrical and asymmetrical TSP instances, which were taken from the Traveling Salesman Problem Library (TSPLIB. The analyzed approaches were divided into three methods: (i Lin-Kernighan-Helsgaun (LKH algorithm; (ii LKH with initial tour based on uniform distribution; and (iii an hybrid proposal combining Particle Swarm Optimization (PSO with quantum inspired behavior and LKH for local search procedure. The tested algorithms presented promising results in terms of computational cost and solution quality.

  15. Minerals detection for hyperspectral images using adapted linear unmixing: LinMin

    CERN Document Server

    Frederic, Schmidt; Stephane, Le Mouelic

    2014-01-01

    Minerals detection over large volume of spectra is the challenge addressed by current hyperspectral imaging spectrometer in Planetary Science. Instruments such OMEGA (Mars Express), CRISM (Mars Reconnaissance Orbiter), M^{3} (Chandrayaan-1), VIRTIS (Rosetta) and many more, have been producing very large datasets since one decade. We propose here a fast supervised detection algorithm called LinMin, in the framework of linear unmixing, with innovative arrangement in order to treat non-linear cases due to radiative transfer in both atmosphere and surface. We use reference laboratory and synthetic spectral library. Additional spectra are used in order to mimic the effect of Martian aerosols, grain size, and observation geometry discrepancies between reference and observed spectra. The proposed algorithm estimates the uncertainty on mixing coefficient from the uncertainty of observed spectra. Both numerical and observational tests validate the approach. Fast parallel implementation of the best algorithm (IPLS) on ...

  16. José Lins do Rego: um guia brasileiro de Israel

    Directory of Open Access Journals (Sweden)

    Glauber Pereira Quintão

    2010-03-01

    Full Text Available Este artigo procura analisar, de forma crítica, o livro de José Lins do Rego, Roteiro de Israel, 1955. Busca-­se destacar duas facetas suplementares: a de um olhar poético e a de uma preocupação ético-política lançados pelo escritor sobre a construção do novo Estado de Israel. Observa-­se também uma relação entre os signos da modernidade e das histórias arcaica judaicas. Para isso, usa-­se o conceito de palimpsesto, de Gerard Genette, e de flâneur, de Walter Benjamim.

  17. An Effective Implementation of K-opt Moves for the Lin-Kernighan TSP Heuristic

    DEFF Research Database (Denmark)

    Helsgaun, Keld

    Local search with k-change neighborhoods, k-opt, is the most widely used heuristic method for the traveling salesman problem (TSP). This report presents an effective implementation of k-opt for the Lin- Kernighan TSP heuristic. The effectiveness of the implementation is demonstrated with extensive...

  18. Lin28B promotes Müller glial cell de-differentiation and proliferation in the regenerative rat retinas

    Science.gov (United States)

    Tao, Zui; Zhao, Chen; Jian, Qian; Gillies, Mark; Xu, Haiwei; Yin, Zheng Qin

    2016-01-01

    Retinal regeneration and repair are severely impeded in higher mammalian animals. Although Müller cells can be activated and show some characteristics of progenitor cells when injured or under pathological conditions, they quickly form gliosis scars. Unfortunately, the basic mechanisms that impede retinal regeneration remain unknown. We studied retinas from Royal College of Surgeon (RCS) rats and found that let-7 family molecules, let-7e and let-7i, were significantly overexpressed in Müller cells of degenerative retinas. It demonstrated that down-regulation of the RNA binding protein Lin28B was one of the key factors leading to the overexpression of let-7e and let-7i. Lin28B ectopic expression in the Müller cells suppressed overexpression of let-7e and let-7i, stimulated and mobilized Müller glia de-differentiation, proliferation, promoted neuronal commitment, and inhibited glial fate acquisition of de-differentiated Müller cells. ERG recordings revealed that the amplitudes of a-wave and b-wave were improved significantly after Lin28B was delivered into the subretinal space of RCS rats. In summary, down-regulation of Lin28B as well as up-regulation of let-7e and let-7i may be the main factors that impede Müller cell de-differentiation and proliferation in the retina of RCS rats. PMID:27384999

  19. Antifungal activity of extracts of Rosmarinus officinalis and Thymus vulgaris against Aspergillus flavus and A. ochraceus.

    Science.gov (United States)

    Centeno, S; Calvo, M A; Adelantado, C; Figueroa, S

    2010-05-01

    The antifungal activity of ethanolic extracts of Rosmarinus officinalis and Thymus vulgaris were tested against strains of Aspergillus flavus and A. ochraceus, since these two species are common contaminants of cereals and grains and are able to produce and accumulate mycotoxins. The methodology used is based on measuring the inhibition halos produced by discs impregnated with the extracts and establishing their Minimum Inhibitory Concentration (MIC) as well as the Minimum Fungicide Concentration (MFC). The results obtained suggest that the assayed extracts affect the proper development of A. flavus and A. ochraceus; leading to a lower MIC (1200 ppm) and MFC (2400 ppm) for T. vulgaris extract against A. ochraceus than against A. flavus. The results show, that the extracts of Rosmarinus officinalis and Thymus vulgaris used at low concentrations could have significant potential for the biological control of fungi in foodstuffs.

  20. In vitro synergistic antibacterial activity of Melissa officinalis L. and some preservatives

    Energy Technology Data Exchange (ETDEWEB)

    Stanojeic, D.; Comic, L.; Stefanovic, O.; Solujic Sukdolak, S.

    2010-07-01

    The aim of this study was to investigate the antibacterial activity of aqueous, ethanol and ethyl acetate extracts of the species Melissa officinalis L. and their in vitro synergistic action with preservatives, namely: sodium nitrite, sodium benzoate and potassium sorbate against selected food spoiling bacteria, for a potential use in food industry. Synergistic action was noticed in almost every combination between plant extracts and preservatives. This work showed that the active compounds from ethanol, ethyl acetate and aqueous extracts of Melissa officinalis L. significantly enhanced the effectiveness of tested preservatives. Synergism was established at plant extract and preservative concentrations corresponding to 1/4 and 1/8 minimal inhibitory concentration values, which indicated the possibility of avoiding the use of higher concentrations of tested preservatives. (Author) 25 refs.

  1. Enzyme assisted extraction of polysaccharides from the fruit of Cornus officinalis.

    Science.gov (United States)

    You, Qinghong; Yin, Xiulian; Zhao, Yuping

    2013-10-15

    Process of enzyme assisted extraction (EAE) of polysaccharides from Cornus officinalis was optimized by response surface methodology (RSM). The influence of four different factors on the yield of C. officinalis polysaccharides (COP) was studied. Results showed that the optimal conditions were compound enzyme amount of 2.15%, extraction pH of 4.2, extraction temperature of 55 °C and extraction time of 97 min. Under these conditions, the COP yield was 9.29±0.31%, which was well in agreement with the value predicted by the model. The three methods, EAE, hot water extraction (HWE), ultrasound-assisted extraction (UAE) for extracting COP by RSM were further compared. Results showed that EAE had the largest yield of polysaccharides with lower equipment cost.

  2. XSHOOTER spectroscopy of the enigmatic planetary nebula Lin49 in the Small Magellanic Cloud

    Science.gov (United States)

    Otsuka, Masaaki; Kemper, F.; Leal-Ferreira, M. L.; Aleman, I.; Bernard-Salas, J.; Cami, J.; Ochsendorf, B. B.; Peeters, E.; Scicluna, P.

    2016-10-01

    We performed a detailed spectroscopic analysis of the fullerene C60-containing planetary nebula (PN) Lin49 in the Small Magellanic Cloud (SMC) using XSHOOTER at the European Southern Observatory Very Large Telescope and the Spitzer/Infrared Spectrograph instruments. We derived nebular abundances for nine elements. We used TLUSTY to derive photospheric parameters for the central star. Lin49 is C-rich and metal-deficient PN (Z ˜ 0.0006). The nebular abundances are in good agreement with asymptotic giant branch nucleosynthesis models for stars with initial mass 1.25 M⊙ and metallicity Z = 0.001. Using the TLUSTY synthetic spectrum of the central star to define the heating and ionizing source, we constructed the photoionization model with CLOUDY that matches the observed spectral energy distribution (SED) and the line fluxes in the UV to far-IR wavelength ranges simultaneously. We could not fit the ˜1-5 μm SED using a model with 0.005-0.1-μm-sized graphite grains and a constant hydrogen density shell owing to the prominent near-IR excess, while at other wavelengths the model fits the observed values reasonably well. We argue that the near-IR excess might indicate either (1) the presence of very small particles in the form of small carbon clusters, small graphite sheets, or fullerene precursors, or (2) the presence of a high-density structure surrounding the central star. We found that SMC C60 PNe show a near-IR excess component to lesser or greater degree. This suggests that these C60 PNe might maintain a structure nearby their central star.

  3. Study on Oligosaccharides from Morinda officinalis%巴戟天寡糖研究

    Institute of Scientific and Technical Information of China (English)

    冯峰; 王羚郦; 赖小平; 李远彬; 曹志梅; 周应军

    2012-01-01

    Objective: To study the oligosaccharides from Morinda officinalis How. Methods: Compounds were isolated by chroma-tography, and their structures were identified by spectral analysis and chemical evidences. Results: six compounds were isolated and i-dentified as sucrose( I ) , inulin-type trisaecharide( II ) , inulin-type hexasaccharide( III) , inulotriose( IV) , inulotetraose( V ) , inu-lopentaose( VI).Conclusion: Compound IV ,V and VI are isolated from Morinda officinalis for the first time. Morinda officinalis How; Oligosaccharides; Inulin-type oligosaccharide; Inulo-type oligosaccharide%目的:对巴戟天(Morinda officinalis)的糖类成分进行化学研究.方法:用活性炭、纤维素和硅胶等色谱法分离,用波谱法对化合物结构进行鉴定.结果:从巴戟天活性部位中分离出6个寡糖类化合物,分别为蔗糖(Ⅰ)、耐斯糖(Ⅱ)、菊粉六糖(Ⅲ)、β-D-果吡喃糖-(2→1)-β-D-果呋喃糖-(2→1)-β-D-果呋喃糖(Ⅳ)、β-D-果吡喃糖-(2→1) -β-D-果呋喃糖-(2→1)-β-D-果呋喃糖-(2→1)-β-D-果呋喃糖(Ⅴ)、β-D-果吡喃糖-(2→1)-β-D-果呋喃糖-( 2→1)-β-D-果呋喃糖-(2→1)-β-D-果呋喃糖-(2→1)-β-D-果呋喃糖(Ⅵ).结论:化合物Ⅳ-Ⅵ为首次从巴戟天属中分离出的化合物.

  4. Composition and insect attracting activity of the essential oil of Rosmarinus officinalis.

    Science.gov (United States)

    Katerinopoulos, Haralambos E; Pagona, Georgia; Afratis, Athanasios; Stratigakis, Nicolaos; Roditakis, Nikolaos

    2005-01-01

    The essential oil and a number of extracts of Rosmarinus officinalis L. in solvents of increasing polarity were isolated, and their components identified and tested as pest control agents. Ethanol and acetone extracts attract grape berry moth Lobesia botrana. However, none of the extracts had a significant effect on western flower thrips Frankliniella occidentalis, which is attracted by 1,8-cineole, a major essential oil component.

  5. THE ESSENTIAL OIL OF LEMON BALM (Melissa officinalis L.), ITS COMPONENTS AND USING FIELDS

    OpenAIRE

    BAHTİYARCA BAĞDAT, Reyhan; Coşge, Belgin

    2012-01-01

    Lemon balm (Melissa officinalis L.), member of Lamiaceae (formerly Labiatae) family, is one of the important medicinal plant species. Today, it is used in different branches of industry (such as medicine, perfume, cosmetic, and food etc.) in many countries of the world. The main components of lemon balm essential oil, ranged from 0.01 to 0.25%, are 39% citronellal, 33% citral (citronellol, linalool) and geraniol. It is traditionally used as a mild sedative, spasmolytic and antibacterial agent...

  6. Hybrid magnetite nanoparticles/ Rosmarinus officinalis essential oil nanobiosystem with antibiofilm activity

    Science.gov (United States)

    Chifiriuc, Carmen; Grumezescu, Valentina; Grumezescu, Alexandru Mihai; Saviuc, Crina; Lazăr, Veronica; Andronescu, Ecaterina

    2012-04-01

    Biofilms formed by fungal organisms are associated with drastically enhanced resistance against most antimicrobial agents, contributing to the persistence of the fungi despite antifungal therapy. The purpose of this study is to combine the unique properties of nanoparticles with the antimicrobial activity of the Rosmarinus officinalis essential oil in order to obtain a nanobiosystem that could be pelliculised on the surface of catheter pieces, in order to obtain an improved resistance to microbial colonization and biofilm development by Candida albicans and C. tropicalis clinical strains. The R. officinalis essential oils were extracted in a Neo-Clevenger type apparatus, and its chemical composition was settled by GC-MS analysis. Functionalized magnetite nanoparticles of up to 20 nm size had been synthesized by precipitation method adapted for microwave conditions, with oleic acid as surfactant. The catheter pieces were coated with suspended core/shell nanoparticles (Fe3O4/oleic acid:CHCl3), by applying a magnetic field on nanofluid, while the CHCl3 diluted essential oil was applied by adsorption in a secondary covering treatment. The fungal adherence ability was investigated in six multiwell plates, in which there have been placed catheters pieces with and without hybrid nanoparticles/essential oil nanobiosystem pellicle, by using culture-based methods and confocal laser scanning microscopy (CLSM). The R. officinalis essential oil coated nanoparticles strongly inhibited the adherence ability and biofilm development of the C. albicans and C. tropicalis tested strains to the catheter surface, as shown by viable cell counts and CLSM examination. Due to the important implications of C andida spp. in human pathogenesis, especially in prosthetic devices related infections and the emergence of antifungal tolerance/resistance, using the new core/shell/coated shell based on essential oil of R. officinalis to inhibit the fungal adherence could be of a great interest for the

  7. Antibacterial activity and characterization of secondary metabolites isolated from mangrove plant Avicennia officinalis

    Institute of Scientific and Technical Information of China (English)

    Valentin Bhimba B; J Meenupriya; Elsa Lycias Joel; D Edaya Naveena; Suman kumar; M Thangaraj

    2010-01-01

    Objective:To explore antibacterial activity and characterization of secondary metabolites isolated from mangrove plant Avicennia officinalis (A. officinalis). Methods:In the present study the leaf extracts of A. officinalis were examined for its antibacterial potential using five different solvents against some reference strains of human pathogenic bacteria for the crude extract. Maximum activity was observed for ethyl acetate and hence different concentrations like 15μL, 25μL, and 50μL of ethyl extracts was checked for its antibacterial activity. Partial purification of crude extract was carried by column chromatography and fractions were analyzed using gas chromatography-mass spectrometry (GC-MS) to identify compounds. Results:The crude ethyl acetate extracts of A. officinalis showed remarkable antibacterial activity with zones of inhibition of 13 mm against Eschericia coli (E. coli) and 11 mm against Staphylococcus aureus (S. aureus). Fraction 13 (ethyl acetate÷methanol=8÷2) as the most potent one against with the minimal inhibitory concentration of 30 mm against E. coli and 25 mm against S. aureus. The GC-MS resultsof active column fraction (F13) revealed that the active principals were a mixture of hydroxy-4 methoxybenzoic acid, diethyl phthalate, oleic acid. Conclusions:The leaf extracts with proven antibacterial effects can clearly be directed towards cancer treatment as to inhibiting cancer cell growth. The limited number of test organisms owes to a constraint of resource. So, the effect of strong bursts of leaf extracts on human pathogenic bacteria should further be tested on a wide range of test organisms.

  8. Carnosol and carnosic acids from Salvia officinalis inhibit microsomal prostaglandin E2 synthase-1.

    Science.gov (United States)

    Bauer, Julia; Kuehnl, Susanne; Rollinger, Judith M; Scherer, Olga; Northoff, Hinnak; Stuppner, Hermann; Werz, Oliver; Koeberle, Andreas

    2012-07-01

    Prostaglandin E(2) (PGE(2)), the most relevant eicosanoid promoting inflammation and tumorigenesis, is formed by cyclooxygenases (COXs) and PGE(2) synthases from free arachidonic acid. Preparations of the leaves of Salvia officinalis are commonly used in folk medicine as an effective antiseptic and anti-inflammatory remedy and possess anticancer activity. Here, we demonstrate that a standard ethyl acetate extract of S. officinalis efficiently suppresses the formation of PGE(2) in a cell-free assay by direct interference with microsomal PGE(2) synthase (mPGES)-1. Bioactivity-guided fractionation of the extract yielded closely related fractions that potently suppressed mPGES-1 with IC(50) values between 1.9 and 3.5 μg/ml. Component analysis of these fractions revealed the diterpenes carnosol and carnosic acid as potential bioactive principles inhibiting mPGES-1 activity with IC(50) values of 5.0 μM. Using a human whole-blood assay as a robust cell-based model, carnosic acid, but not carnosol, blocked PGE(2) generation upon stimulation with lipopolysaccharide (IC(50) = 9.3 μM). Carnosic acid neither inhibited the concomitant biosynthesis of other prostanoids [6-keto PGF(1α), 12(S)-hydroxy-5-cis-8,10-trans-heptadecatrienoic acid, and thromboxane B(2)] in human whole blood nor affected the activities of COX-1/2 in a cell-free assay. Together, S. officinalis extracts and its ingredients carnosol and carnosic acid inhibit PGE(2) formation by selectively targeting mPGES-1. We conclude that the inhibitory effect of carnosic acid on PGE(2) formation, observed in the physiologically relevant whole-blood model, may critically contribute to the anti-inflammatory and anticarcinogenic properties of S. officinalis.

  9. Identification of benzoin obtained from calli of Styrax officinalis by HPLC

    OpenAIRE

    DEMİRAY, Hatice; DEREBOYLU, Aylin EŞİZ; YAZICI, Zekiye Işın; Karabey, Fatih

    2013-01-01

    The stem tissue of Styrax officinalis L. distributed in West Anatolia was induced with agents such as boric acid and cocarboxylase (thiamine diphosphate), extensive stimulators of resin channels, in order to increase the amount of benzoin volatile oil. While the benzoin content was 120% in the induction medium to which excess boron and niacin were added, it increased to 231% when cocarboxylase (thiamine diphosphate) was added to the medium. Benzoin content of Styrax from petiole calli was 166...

  10. Terpene Glycosides from the Roots of Sanguisorba officinalis L. and Their Hemostatic Activities

    OpenAIRE

    Wei Sun; Zi-Long Zhang; Xin Liu; Shuang Zhang; Lu He; Zhe Wang; Guang-Shu Wang

    2012-01-01

    Guided by a hemostasis bioassay, seven terpene glycosides were isolated from the roots of Sanguisorba officinalis L. by silica gel column chromatography and preparative HPLC. On the grounds of chemical and spectroscopic methods, their structures were identified as citronellol-1-O-α-l-arabinofuranosyl-(1→6)-β-d-glucopyranoside (1), geraniol-1-O-α-l-arabinofuranosyl-(1→6)-β-d-glucopyranoside (

  11. Mechanical properties and total hydroxycinnamic derivative release of starch/glycerol/Melissa officinalis extract films

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    Letícia Mello Rechia

    2010-09-01

    Full Text Available The aim of this study was to investigate the mechanical properties of starch/glycerol/Melissa officinalis, a topical drug delivery system for labial herpes treatment. Four films were prepared with different concentrations of starch, glycerol, and Melissa officinalis extract. The results revealed that increasing the glycerol concentration in the film reduced elasticity modulus and tensile strength, exhibiting a plasticizing effect. The increase in free volume resulted in increased release of hydroxycinnamic derivatives expressed as rosmarinic acid.O objetivo deste trabalho foi estudar as propriedades mecânicas e o mecanismo de liberação de um sistema tópico de liberação prolongada para o tratamento do Herpes labial a partir de filmes de amido/glicerol/extrato de Melissa officinalis, planta com comprovada atividade antiviral. Foram obtidos quatro filmes poliméricos com diferentes concentrações de amido, glicerol e extrato de Melissa officinalis os quais foram caracterizados mecanicamente e determinado o perfil de liberação de derivados hidroxicinâmicos. Os resultados demonstraram que o aumento da concentração de glicerol no filme produz uma redução no módulo de elasticidade e na tensão de deformação como conseqüência do efeito plastificante. O aumento no volume livre do polímero resultou em aumento da liberação dos derivados hidroxicinâmicos expressos como ácido rosmarínico.

  12. Preliminary phytochemical,acute oral toxicity and antihepatotoxic study of roots of Paeonia officinalis Linn.

    Institute of Scientific and Technical Information of China (English)

    Feroz; Ahmad; Nahida; Tabassum

    2013-01-01

    Objective:To carry out a preliminary phytochemical,acute oral toxicity and antihepatotoxic study of the roots of Paeonia officinalis(P.officinalis)L.Methods:Preliminary phytochemical investigation was done as per standard procedures.Acute oral toxicity study was conducted as per OECD 425 guidelines.The antihepatotoxic activity of aqueous extract of root of P.officinalis was evaluated against carbon tetrachloride(CCl4)induced hepatic damage in rats.Aqueous extract of P.officinalis at the dose levels of 100 and 200 mg/kg body weight was administered daily for 14 d in experimental animals.Liver injury was induced chemically,by CCl4 administration(1 mL/kg i.p.).The hepatoprotective activity was assessed using various biochemical parameters like aspartate aminotransferase(AST),alanine aminotransferase(ALT),serum alkaline phosphatase(SALP),total bilirubin and total protein(TP)along with histopathological studies.Result:Phytochemical screening revealed that the roots of P.officinalis contain alkaloids,tannins,saponins,glycosides,carbohydrates,flavonoids,terpenes,steroids and proteins.The aqueous extract did not cause any mortality up to 2000 mg/kg.In rats that had received the root extract at the dose of 100 and 200 mg/kg,the substantially elevated AST,ALT,SALP,total bilirubin levels were significantly lowered,respectively,in a dose dependent manner,along with CCl4 while TP levels were elevated in these groups.Histopathology revealed regeneration of the livers in extract treated groups while Silymarin treated rats were almost normal.Conclusions:The aqueous extract of P.officinalis is safe and possesses antihepatotoxic potential.

  13. Identification of Magnolia officinalis L. bark extract as the most potent anti-inflammatory of four plant extracts.

    Science.gov (United States)

    Walker, Joel M; Maitra, Amarnath; Walker, Jessica; Ehrnhoefer-Ressler, Miriam M; Inui, Taichi; Somoza, Veronika

    2013-01-01

    This study was designed to compare the anti-inflammatory potential of a Magnolia officinalis L. bark extract solely or in combination with extracts prepared from either Polygonum aviculare L., Sambucus nigra L., or Isodon japonicus L. in bacterial lipopolysaccharide (LPS) stimulated human gingival fibroblasts (HGF-1) and human U-937 monocytes, as cell models of periodontal disease. HGF-1 and U-937 cells were incubated with LPS from either Porphyromonas gingivalis or Escherichia coli together with the four plant extracts alone or in combination. Secretion of anti-inflammatory cytokines from HGF-1 and U-937 cells was measured by means of a multiplexed bead assay system. Magnolia officinalis L. bark extract, at concentrations of 1 μg/mL and 10 μg/mL, reduced interleukin 6 (IL-6) and interleukin-8 (IL-8) secretion from HGF-1 cells to 72.5 ± 28.6% and reduced matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9) secretion from U-937 cells to 8.87 ± 7.97% compared to LPS-treated cells (100%). The other three extracts also reduced secretion of these inflammatory markers but were not as effective. Combination of 9 μg/mL Magnolia officinalis L. extract with 1 μg/mL of each of the other extracts maintained the anti-inflammatory effect of Magnolia officinalis L. extract. Combination of 5 μg/mL Magnolia officinalis L. extract with 5 μg/mL Isodon japonicus L. extract also maintained the anti-inflammatory potential of the Magnolia officinalis L. extract, whereas increasing concentrations of any of the other plant extracts in the combination experiments reduced the Magnolia officinalis L. extract efficacy in U-937 cells.

  14. CONSTRUCTION AND STUDY OF Althaea officinalis TRANSGENIC ROOTS CULTURE WITH HUMAN INTERFERON α2B GENE

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    N. A. Matvieieva

    2013-04-01

    Full Text Available The aim of our work was to obtain Althaea officinalis L. «hairy» root culture with human interferon α2b gene (ifn-α2b, to measure fructans content and antiviral activity of extracts from the transgenic roots. Transformation of leaf and root explants was carried out by means of Agrobacterium rhizogenes-mediated transformation. Antiviral activity was measured by the reduction in cytopathic effect of vesicular stomatitis virus (Indiana strain in bovine kidney cells line MDBK. Transformation frequency was 100% for leaf and root explants. RT-PCR confirmed ifn- α2b gene transcription. The clones of transgenic roots differed in mass increasing from 0, 036 ± 0,008 up to 0,371 ± 0,019 g in 30 days cultivation and in fructan synthesis from 67,2± 4,47 up to 154,6 ± 6,62 mg/g roots dry weight. Extracts from «hairy»roots culture were characterized by high antiviral activity against vesicular stomatitis virus — up to 26 000 IU/ g of roots fresh weight. In some cases the genetic transformation shown to lead increasing the growth rate and increasing the level of fructan synthesis in transgenic A. officinalis roots. Extracts from cultivated in vitro marshmallow transgenic roots were characterized by high level of antiviral activity against vesicular stomatitis virus. Thus, there were obtained transgenic A. officinalis roots, characterized by high growth rate, significant accumulation of fructans and high antiviral activity.

  15. Evaluation of the genotoxic and antigenotoxic potential of Melissa officinalis in mice

    Science.gov (United States)

    de Carvalho, Natália Cassettari; Corrêa-Angeloni, Maria Júlia Frydberg; Leffa, Daniela Dimer; Moreira, Jeverson; Nicolau, Vanessa; de Aguiar Amaral, Patrícia; Rossatto, Ângela Erna; de Andrade, Vanessa Moraes

    2011-01-01

    Melissa officinalis (L.) (Lamiaceae), a plant known as the lemon balm, is native to the east Mediterranean region and west Asia. Also found in tropical countries, such as Brazil, where it is popularly known as “erva-cidreira” or “melissa”, it is widely used in aqueous- or alcoholic-extract form in the treatment of various disorders. The aim was to investigate in vivo its antigenotoxicity and antimutagenicity, as well as its genotoxic/mutagenic potential through comet and micronucleus assaying. CF-1 male mice were treated with ethanolic (Mo-EE) (250 or 500 mg/kg) or aqueous (Mo-AE) (100 mg/kg) solutions of an M. officinalis extract for 2 weeks, prior to treatment with saline or Methyl methanesulfonate (MMS) doses by intraperitoneal injection. Irrespective of the doses, no genotoxic or mutagenic effects were observed in blood and bone-marrow samples. Although Mo-EE exerted an antigenotoxic effect on the blood cells of mice treated with the alkylating agent (MMS) in all the doses, this was not so with Mo-AE. Micronucleus testing revealed the protector effect of Mo-EE, but only when administered at the highest dose. The implication that an ethanolic extract of M. officinalis has antigenotoxic/antimutagenic properties is an indication of its medicinal relevance. PMID:21734832

  16. Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells.

    Science.gov (United States)

    Garcia, Charlene S C; Menti, Caroline; Lambert, Ana Paula F; Barcellos, Thiago; Moura, Sidnei; Calloni, Caroline; Branco, Cátia S; Salvador, Mirian; Roesch-Ely, Mariana; Henriques, João A P

    2016-03-01

    Salvia officinalis (Lamiaceae) has been used in south of Brazil as a diary homemade, in food condiment and tea-beverage used for the treatment of several disorders. The objective of this study was to characterize chemical compounds in the hydroalcoholic (ExtHS) and aqueous (ExtAS) extract from Salvia officinalis (L.) by gas chromatography-mass spectrometry (GC-MS) and by high-resolution electrospray ionization mass spectrometry (ESI-QTOF MS/MS), evaluate in vitro ability to scavenge the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), catalase (CAT-like) and superoxide dismutase (SOD-like) activity, moreover cytotoxic by MTT assay, alterations on cell morphology by giemsa and apoptotic-induced mechanism for annexin V/propidium iodide. Chemical identification sage extracts revealed the presence of acids and phenolic compounds. In vitro antioxidant analysis for both extracts indicated promising activities. The cytotoxic assays using tumor (Hep-2, HeLa, A-549, HT-29 and A-375) and in non-tumor (HEK-293 and MRC-5), showed selectivity for tumor cell lines. Immunocytochemistry presenting a majority of tumor cells at late stages of the apoptotic process and necrosis. Given the results presented here, Brazilian Salvia officinalis (L.) used as condiment and tea, may protect the body against some disease, in particularly those where oxidative stress is involved, like neurodegenerative disorders, inflammation and cancer.

  17. Effects of Rosmarinus officinalis on the survivability of random-patterned skin flaps: an experimental study.

    Science.gov (United States)

    Ince, Bilsev; Yildirim, Alpagan Mustafa; Okur, Mehmet Ihsan; Dadaci, Mehmet; Yoruk, Ebru

    2015-04-01

    Improving survival of skin flaps used in soft-tissue reconstruction is clinically an important goal, and several systemic and local agents have been used for this purpose. However, a substance that prevents the flap necrosis has not yet been defined. This study aimed to investigate whether a Rosmarinus officinalis extract could improve the skin flap survival. In this study, 21 Wistar albino rats were divided into three groups. Rectangular 8 × 2 cm random-pattern flaps were elevated from the back of the rats. Group I was considered the control group. In Group II, a 0.5-cc of Rosmarinus officinalis oil was applied with an ear bud to the flap area 30 minutes before the flap elevation. After suturing the flaps to their location, the oil was administered twice a day for a week. In Group III, 0.5 cc of the oil was applied twice a day to the area that was elevated for a week until surgery. At the end of the week, the flaps were sutured to their location, and wiped postoperatively twice a day for a week with the oil. Mean percentage of these areas was found to be 29.81%, 58.99%, and 67.68% in Group I, Group II, and Group III, respectively. The mean percentage of the flap survival areas and vessel diameters were significantly greater in the Groups II and III than in the control group (p Rosmarinus officinalis extract can increase the flap survivability.

  18. Variations in essential oil, phenolic compounds, and antioxidant activity of tunisian cultivated Salvia officinalis L.

    Science.gov (United States)

    Ben Farhat, Mouna; Jordán, María J; Chaouech-Hamada, Rym; Landoulsi, Ahmed; Sotomayor, Jose A

    2009-11-11

    The variation in the chemical composition of the essential oil of Salvia officinalis , growing in different habitats, was studied. GC-MS analysis revealed 57 compounds representing 94.68-96.80% of total oils. The major components were alpha-thujone (11.55-19.23%), viridiflorol (9.94-19.46%), 1,8-cineole (8.85-15.60%), camphor (5.08-15.06%), manool (5.52-13.06%), beta-caryophyllene (2.63-9.24%), alpha-humulene (1.93-8.94%), and beta-thujone (5.45-6.17%), showing significant differences between different collection sites. Analysis of some representative polyphenolic compounds and antioxidant activity was performed using postdistilled dry samples. Rosmarinic acid, carnosol, and carnosic acid were the prevalent compounds of S. officinalis methanolic extracts. The results revealed differences in the polyphenolic composition and also exhibited antioxidant and radical-scavenging activities at different magnitudes of potency. However, within the used methods, only the DPPH(*) assay showed significant differences (p officinalis might be valuable antioxidant natural sources and seemed to be applicable in both the health medicine and food industries.

  19. Disinfectant properties of essential oils from Salvia officinalis L. cultivated in Tunisia.

    Science.gov (United States)

    Bouaziz, Mohamed; Yangui, Thabèt; Sayadi, Sami; Dhouib, Abdelhafidh

    2009-11-01

    The essential oils were obtained by hydro-distillation of the aerial part of Salvia officinalis L. cultivated in Sfax gardens, Tunisia. The obtained oils were analyzed by gas chromatography-mass spectrometry (GC-MS) and 44 compounds were identified. Strong bactericidal and fungicidal effects were shown using the NCCLS broth dilution, EN 1275 and EN 1276 standard methods. The minimal cidal concentrations (MCCs) values ranged from 0.031 to 0.25 microLmL(-1). The essential oils concentrations of 0.5% and 1% (v/v) resulted in a reduction in viability higher than 5 and 4 log units per mL for the standard bacteria and fungi, respectively, within a contact time of 5 min. Using an air sampler and an aroma dispenser, vaporisation of 0.25 mLm(-3) of S. officinalis essential oils resulted in (72%, 73% and 70%) and (54%, 55% and 55%) reduction of the total microbial count and the total count of yeasts and moulds, after a residence time of 1h, 6h and 24h in a selected testing room, respectively. S. officinalis essential oils showed a potent vapour activity against a panel of bacteria, yeasts and fungi. This supported their use as a natural eco-friendly disinfectant to manage airborne microbes.

  20. Color blindness and contrast perception in cuttlefish (Sepia officinalis) determined by a visual sensorimotor assay.

    Science.gov (United States)

    Mäthger, Lydia M; Barbosa, Alexandra; Miner, Simon; Hanlon, Roger T

    2006-05-01

    We tested color perception based upon a robust behavioral response in which cuttlefish (Sepia officinalis) respond to visual stimuli (a black and white checkerboard) with a quantifiable, neurally controlled motor response (a body pattern). In the first experiment, we created 16 checkerboard substrates in which 16 grey shades (from white to black) were paired with one green shade (matched to the maximum absorption wavelength of S. officinalis' sole visual pigment, 492 nm), assuming that one of the grey shades would give a similar achromatic signal to the tested green. In the second experiment, we created a checkerboard using one blue and one yellow shade whose intensities were matched to the cuttlefish's visual system. In both assays it was tested whether cuttlefish would show disruptive coloration on these checkerboards, indicating their ability to distinguish checkers based solely on wavelength (i.e., color). Here, we show clearly that cuttlefish must be color blind, as they showed non-disruptive coloration on the checkerboards whose color intensities were matched to the Sepia visual system, suggesting that the substrates appeared to their eyes as uniform backgrounds. Furthermore, we show that cuttlefish are able to perceive objects in their background that differ in contrast by approximately 15%. This study adds support to previous reports that S. officinalis is color blind, yet the question of how cuttlefish achieve "color-blind camouflage" in chromatically rich environments still remains.

  1. Impact of different culture media on hairy roots growth of Valeriana officinalis L.

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    Ali PAKDIN PARIZI

    2015-12-01

    Full Text Available Transformed hairy root cultures of Valeriana officinalis were established by infection with Agrobacterium rhizogenes strain ATCC 15834. To determine the effect of different media on the growth of V. officinalis hairy roots, MS, B5 media (1.0X and 0.5X strength, N6 medium and a modified MS medium without phytohormones were used. In addition, different NH4+ to NO3- ratios in MS medium were studied. The effects of these treatments were evaluated after 21 days of culture in relation to hairy root growth. B5 and ½ B5 media were the best basal media for hairy root growth. MS medium supplemented with a 20:20 ratio (mM of NH4+ to NO3- displayed highest growth rates and biomass yield in hairy root cultures. The present study demonstrated that the composition of culture medium and the ratio of different nitrogen sources have significant impact on the growth of V. officinalis hairy roots.

  2. Neuroprotective properties of Melissa officinalis after hypoxic-ischemic injury both in vitro and in vivo

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    Bayat Mohammad

    2012-10-01

    Full Text Available Abstract Background Brain ischemia initiates several metabolic events leading to neuronal death. These events mediate large amount of damage that arises after some neurodegenerative disorders as well as transient brain ischemia. Melissa officinalis is considered as a helpful herbal plant in the prevention of various neurological diseases like Alzheimer that is related with oxidative stress. Methods We examined the effect of Melissa officinalis on hypoxia induced neuronal death in a cortical neuronal culture system as in vitro model and transient hippocampal ischemia as in vivo model. Transient hippocampal ischemia was induced in male rats by tow vessel-occlusion for 20 min. After reperfusion, the histopathological changes and the levels inflammation, oxidative stress status, and caspase-3 activity in hippocampus were measured. Results Cytotoxicity assays showed a significant protection of a 10 μg/ml dose of Melissa against hypoxia in cultured neurons which was confirmed by a conventional staining (P Discussion Results showed that Melissa officinalis could be considered as a protective agent in various neurological diseases associated with ischemic brain injury.

  3. Neuroprotective properties of Melissa officinalis after hypoxic-ischemic injury both in vitro and in vivo

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    Mahnaz Khanavi

    2012-10-01

    Full Text Available Brain ischemia initiates several metabolic events leading to neuronal death. These events mediate large amount of damage that arises after some neurodegenerative disorders as well as transient brain ischemia. Melissa officinalis is considered as a helpful herbal plant in the prevention of various neurological diseases like Alzheimer that is related with oxidative stress.MethodsWe examined the effect of Melissa officinalis on hypoxia induced neuronal death in a cortical neuronal culture system as in vitro model and transient hippocampal ischemia as in vivo model. Transient hippocampal ischemia was induced in male rats by tow vessel-occlusion for 20 min. After reperfusion, the histopathological changes and the levels inflammation, oxidative stress status, and caspase-3 activity in hippocampus were measured.ResultsCytotoxicity assays showed a significant protection of a 10 mug/ml dose of Melissa against hypoxia in cultured neurons which was confirmed by a conventional staining (P<0.05. Melissa treatment decrease caspase3 activity (P<0.05 and TUNEL-positive cells significantly (P<0.01. Melissa oil has also inhibited malon dialdehyde level and attenuated decrease of Antioxidant Capacity in the hippocampus. Pro-inflammatory cytokines TNF-alpha, IL-1beta and HIF-1alpha mRNA levels were highly increased after ischemia and treatment with Melissa significantly suppressed HIF-1alpha gene expression (P<0.05.DiscussionResults showed that Melissa officinalis could be considered as a protective agent in various neurological diseases associated with ischemic brain injury.

  4. Evaluation of larvicidal activity of a nanoemulsion of Rosmarinus officinalis essential oil

    Directory of Open Access Journals (Sweden)

    Jonatas L. Duarte

    2015-04-01

    Full Text Available Abstract Nanotechnology has emerged as a promising area for innovative products, including insecticides. Dengue is a tropical disease which is considered a critical health problem in developing countries, due to negative impacts to the environment caused by synthetic chemicals used for vector control (Aedes aegypti. Thus, developing of natural products based insecticidal are considered very promising. On this context, the aim of the present study was to obtain an O/W nanoemulsion containing Rosmarinus officinalis L., Lamiaceae, essential oil and evaluate its larvicidal activity against A. aegypti. Low energy method was employed, allowing achievement of small droplets. The nanoemulsion also presented low polydispersity and mean droplet below 200 nm, even after 30 days of storage. Potential mortality levels were observed after 24 h (80 ± 10% and 48 h (90 ± 10% in A. aegypti larvae at final concentration of 250 ppm, related to R. officinalis essential oil. This study contributes to nanobiotechnology of natural products, presenting a potential larvicidal nanoemulsion prepared with R. officinalis essential oil. Moreover, nanoemulsion production involved a non-heating procedure, describing easy technique which may be useful for integrative control programs.

  5. [Analysis of acid rain characteristics of Lin'an Regional Background Station using long-term observation data].

    Science.gov (United States)

    Li, Zheng-Quan; Ma, Hao; Mao, Yu-Ding; Feng, Tao

    2014-02-01

    Using long-term observation data of acid rain at Lin'an Regional Background Station (Lin'an RBS), this paper studied the interannual and monthly variations of acid rain, the reasons for the variations, and the relationships between acid rain and meteorological factors. The results showed that interannual variation of acid rain at Lin'an RBS had a general increasing trend in which there were two obvious intensifying processes and two distinct weakening processes, during the period ranging from 1985 to 2012. In last two decades, the monthly variation of acid rain at Lin'an RBS indicated that rain acidity and frequency of severe acid rain were increasing but the frequency of weak acid rain was decreasing when moving towards bilateral side months of July. Acid rain occurrence was affected by rainfall intensity, wind speed and wind direction. High frequency of severe acid rain and low frequency of weak acid rain were on days with drizzle, but high frequency of weak acid rain and low frequency of severe acid rain occurred on rainstorm days. With wind speed upgrading, the frequency of acid rain and the proportion of severe acid rain were declining, the pH value of precipitation was reducing too. Another character is that daily dominant wind direction of weak acid rain majorly converged in S-W section ,however that of severe acid rain was more likely distributed in N-E section. The monthly variation of acid rain at Lin'an RBS was mainly attributed to precipitation variation, the increasing and decreasing of monthly incoming wind from SSE-WSW and NWN-ENE sections of wind direction. The interannual variation of acid rain could be due to the effects of energy consumption raising and significant green policies conducted in Zhejiang, Jiangsu and Shanghai.

  6. [Chemical diversity of the biological active ingredients of salvia officinalis and some closely related species].

    Science.gov (United States)

    Máthé, Imre; Hohmann, Judit; Janicsák, Gábor; Nagy, Gábor; Dora, Rédei

    2007-01-01

    Comparative studies on the volatile and non-volatile fractions of 6 species. i.e. Salvia officinalis, S. tomentosa, S. fruticosa, S. candelabrum, S. ringens, S. lavandulifolia of the Section Salvia (Lamiaceae) have been carried out. Both fractions provide the chemical pattern matches to the chemotaxonomic character of Subfamily Nepetoideae in Erdtmanr two subfamiliar system. S. lavandulifolia had the highest essential oil content, followed by S. fruticosa, S. tomentosa, S. officinalis and S. candelabrum. S. ringens contains volatile oil only in traces. The neurotoxin thujone content was the highest in the S. officinalis oils and in that of S. fruticosa. No thujone was detected in S. lavandulifolia. The other species, e.g.: S. tomentosa contain this compound only in moderate concentrations (less than 10%). Among the non-volatile fractions of the plant ingredients the triterpene ursolic and oleanolic acids had the highest concentration in the leaves. Despite some rare cases, ursolic acid dominates the tritepene fraction. Rosmarinic and caffeic acids were measured in similar concentrations, in all species. As the case of S. officinalis shows, these compounds vary significantly in all organs during the vegetation period. Caffeic acid is also ubiquitous in the genus Salvia but as our data suggest it occurs in an order of magnitude lower concentration than rosmarinic acid. The isolation of phenylethanolid martynoside, though obtained in a rather small concentration, is of great chemotaxonomic significance, as this is the first phenylethanolid type glycoside isolated not only from the Salvia genus but also from the entire Subfamily Nepetoideae. As pheylethanolids are rather common and accumulate in significant concentrations in plants of the Subfamily Lamioideae, our opinion that the chemical differences between the two subfamilies are less qualititative than quantitative, is confirmed. This holds true of other chemical markers like monoterpenes, ursolic and oleanolic

  7. Erythroid-Specific Expression of LIN28A Is Sufficient for Robust Gamma-Globin Gene and Protein Expression in Adult Erythroblasts.

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    Y Terry Lee

    Full Text Available Increasing fetal hemoglobin (HbF levels in adult humans remains an active area in hematologic research. Here we explored erythroid-specific LIN28A expression for its effect in regulating gamma-globin gene expression and HbF levels in cultured adult erythroblasts. For this purpose, lentiviral transduction vectors were produced with LIN28A expression driven by erythroid-specific gene promoter regions of the human KLF1 or SPTA1 genes. Transgene expression of LIN28A with a linked puromycin resistance marker was restricted to the erythroid lineage as demonstrated by selective survival of erythroid colonies (greater than 95% of all colonies. Erythroblast LIN28A over-expression (LIN28A-OE did not significantly affect proliferation or inhibit differentiation. Greater than 70% suppression of total let-7 microRNA levels was confirmed in LIN28A-OE cells. Increases in gamma-globin mRNA and protein expression with HbF levels reaching 30-40% were achieved. These data suggest that erythroblast targeting of LIN28A expression is sufficient for increasing fetal hemoglobin expression in adult human erythroblasts.

  8. The effect of Asparagus officinalis polysaccharide on erythrocyte of S180 mice played in immunological reaction of lymphocytes

    Institute of Scientific and Technical Information of China (English)

    JI Chen-feng; MIN Yong-cui; JI Yu-bin

    2008-01-01

    Objective To contrast the roles of Asparagus officinalis polysaccharide on erythroeyte of S180 mice played in immunological reaction of lymphocytes. To study the effect of Asparagus officinalis polysaecharide on the erythroeyte function of S180 mice. Methods Suspensions of lymphocytes (1×106/mL) and autologous plasma were respectively separated from anticoaguted whole blood of healthy mice with the lymphocyte separation medium. The erythrocytes (1×108/mL) were separated from whole blood of Asparagus officinalis polysaccharide mice. Using the autologous plasm as reactive medium, the role of erythrocytes in regulating the immunological reaction of lymphocytes was appraised. The expression of CD25 on lymphocytes was detected using flow cytometry. Results The immunogical regulating ability of erythrocyte in mice with control groups is much lower than that of normal groups, and the immunogical regulating ability of erythroeyte in mice with Asparagus officinalis polysaccharide groups is much higher than that of control groups. Conclusions According to the effects of erythrocyte CD35 on the immuno-response of lymphocyte and the different of the expression of CD25 on lymphocytes, we prove that Asparagus officinalis polysaecharide can improve the erythrocyte function of S180 mice.

  9. Molecular phylogeny of the genus Asparagus (Asparagaceae) explains interspecific crossability between the garden asparagus (A. officinalis) and other Asparagus species.

    Science.gov (United States)

    Kubota, Shosei; Konno, Itaru; Kanno, Akira

    2012-02-01

    The genus Asparagus comprises approximately 200 species, some of which are commercially cultivated, such as the garden asparagus (A. officinalis). Many Asparagus species, including A. officinalis, are dioecious and have been grouped into a subgenus distinct from that of hermaphroditic species. Although many interspecific crossings have been attempted to introduce useful traits into A. officinalis, only some of the dioecious species were found to be cross-compatible with A. officinalis. Here, molecular phylogenetic analyses were conducted to determine whether interspecific crossability is proportional to the genetic distance between the crossing pairs and to further clarify the evolutionary history of the Asparagus genus. A clade with all cross-compatible species and no cross-incompatible species was recovered in the phylogenetic tree based on analyses of non-coding cpDNA regions. In addition, a sex-linked marker developed for A. officinalis amplified a male-specific region in all cross-compatible species. The phylogenetic analyses also provided some insights about the evolutionary history of Asparagus; for example, by indicating that the genus had its origin in southern Africa, subsequently spreading throughout the old world through intensive speciation and dispersal. The results also suggest that dioecious species were derived from a single evolutionary transition from hermaphroditism in Asparagus. These findings not only contribute towards the understanding of the evolutionary history of the genus but may also facilitate future interspecific hybridization programs involving Asparagus species.

  10. Protective effect of Melissa officinalis extract against H2O2-induced oxidative stress in human vascular endothelial cells

    Directory of Open Access Journals (Sweden)

    Leila Safaeian

    2016-01-01

    Full Text Available Melissa officinalis L. is a medicinal plant with a large variety of pharmacological effects and traditional applications. This study aimed to evaluate the protective and antioxidant activities of the extract of M. officinalis aerial parts on human umbilical vein endothelial cells (HUVECs under oxidative stress induced by H 2 O 2 . Cells were incubated with H 2 O 2 (0.5 mM, 2 h after pretreatment with M. officinalis extract (25-500 µg/mL. Cell viability was evaluated by 3-(4, 5- Dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide (MTT assay. The concentration of hydroperoxides and ferric reducing antioxidant power (FRAP were measured in intra- and extra-cellular fluids. Pretreatment of HUVECs with M. officinalis extract at the concentrations of 100-500 µg/mL improved the cell viability after exposure to H 2 O 2 significantly. It also decreased hydroperoxides concentration and increased FRAP value in both intra- and extra-cellular fluids. The results revealed antioxidant and cytoprotective effects of M. officinalis against H 2 O 2 -induced oxidative stress in HUVECs. Due to the valuable antioxidant activity , this plant extract may have potential benefits for the prevention of cardiovascular diseases associated with oxidative stress.

  11. Herbicides for Calendula

    Science.gov (United States)

    The rising need for replacements for volatile organic compounds (VOC), which are used in the manufacture of paints, plastics, pesticides, etc., has placed demands on drying oils that may not be met by current sources. The primary source is eleostearic acid, or tung oil, which is derived from the see...

  12. Analysis of a lin-42/period Null Allele Implicates All Three Isoforms in Regulation of Caenorhabditis elegans Molting and Developmental Timing

    Science.gov (United States)

    Edelman, Theresa L. B.; McCulloch, Katherine A.; Barr, Angela; Frøkjær-Jensen, Christian; Jorgensen, Erik M.; Rougvie, Ann E.

    2016-01-01

    The Caenorhabditis elegans heterochronic gene pathway regulates the relative timing of events during postembryonic development. lin-42, the worm homolog of the circadian clock gene, period, is a critical element of this pathway. lin-42 function has been defined by a set of hypomorphic alleles that cause precocious phenotypes, in which later developmental events, such as the terminal differentiation of hypodermal cells, occur too early. A subset of alleles also reveals a significant role for lin-42 in molting; larval stages are lengthened and ecdysis often fails in these mutant animals. lin-42 is a complex locus, encoding overlapping and nonoverlapping isoforms. Although existing alleles that affect subsets of isoforms have illuminated important and distinct roles for this gene in developmental timing, molting, and the decision to enter the alternative dauer state, it is essential to have a null allele to understand all of the roles of lin-42 and its individual isoforms. To remedy this problem and discover the null phenotype, we engineered an allele that deletes the entire lin-42 protein-coding region. lin-42 null mutants are homozygously viable, but have more severe phenotypes than observed in previously characterized hypomorphic alleles. We also provide additional evidence for this conclusion by using the null allele as a base for reintroducing different isoforms, showing that each isoform can provide heterochronic and molting pathway activities. Transcript levels of the nonoverlapping isoforms appear to be under coordinate temporal regulation, despite being driven by independent promoters. The lin-42 null allele will continue to be an important tool for dissecting the functions of lin-42 in molting and developmental timing. PMID:27729432

  13. Analysis of a lin-42/period Null Allele Implicates All Three Isoforms in Regulation of Caenorhabditis elegans Molting and Developmental Timing.

    Science.gov (United States)

    Edelman, Theresa L B; McCulloch, Katherine A; Barr, Angela; Frøkjær-Jensen, Christian; Jorgensen, Erik M; Rougvie, Ann E

    2016-12-07

    The Caenorhabditis elegans heterochronic gene pathway regulates the relative timing of events during postembryonic development. lin-42, the worm homolog of the circadian clock gene, period, is a critical element of this pathway. lin-42 function has been defined by a set of hypomorphic alleles that cause precocious phenotypes, in which later developmental events, such as the terminal differentiation of hypodermal cells, occur too early. A subset of alleles also reveals a significant role for lin-42 in molting; larval stages are lengthened and ecdysis often fails in these mutant animals. lin-42 is a complex locus, encoding overlapping and nonoverlapping isoforms. Although existing alleles that affect subsets of isoforms have illuminated important and distinct roles for this gene in developmental timing, molting, and the decision to enter the alternative dauer state, it is essential to have a null allele to understand all of the roles of lin-42 and its individual isoforms. To remedy this problem and discover the null phenotype, we engineered an allele that deletes the entire lin-42 protein-coding region. lin-42 null mutants are homozygously viable, but have more severe phenotypes than observed in previously characterized hypomorphic alleles. We also provide additional evidence for this conclusion by using the null allele as a base for reintroducing different isoforms, showing that each isoform can provide heterochronic and molting pathway activities. Transcript levels of the nonoverlapping isoforms appear to be under coordinate temporal regulation, despite being driven by independent promoters. The lin-42 null allele will continue to be an important tool for dissecting the functions of lin-42 in molting and developmental timing.

  14. Screening of radical scavenging activity of some medicinal and aromatic plant extracts

    NARCIS (Netherlands)

    Miliauskas, G.; Venskutonis, R.P.; Beek, van T.A.

    2004-01-01

    Extracts of 12 medicinal and aromatic plants were investigated for their radical scavenging activity using DPPH and ABTS assays: Salvia sclarea, Salvia glutinosa, Salvia pratensis, Lavandula angustifolia, Calendula officinalis, Matricaria recutita, Echinacea purpurea, Rhaponticum carthamoides, Jugla

  15. Lin28B Gene Expression and its Role in the Onset of Puberty of Sheep%Lin28B基因表达与绵羊初情期启动的关系研究

    Institute of Scientific and Technical Information of China (English)

    卢萍平; 邢凤; 刘博丹; 梁志鹏

    2014-01-01

    初情期是家畜从不能繁殖到获得繁殖能力的标志,是雌性动物发育过程中的一个关键阶段。研究以性早熟的多浪羊和性晚熟的和田羊为研究对象,对其 L in28B 基因外显子3进行克隆,采用 Real-time PCR 技术分析 Lin28B 基因在多浪羊和和田羊幼年期、初情期下丘脑、卵巢中表达变化,结果显示多浪羊和和田羊 L in28B 基因外显子3序列相同,多浪羊下丘脑和卵巢中 L in28B 基因 mRNA 表达量在初情期时低于幼年期( P <0.05);和田羊卵巢L in28B 基因 mRNA 表达量在初情期时低于幼年期(P<0.05)。研究结果表明,L in28B 基因表达下调与初情期启动呈正相关,该研究对于揭示 L in28B 基因在绵羊初情期启动中的作用及机制提供了科学依据。%Puberty of animals marked their fertility and is a key stage in female animal development . Precocious sheep breed ,Duolang sheep ,and late-maturing sheep breed ,Hetian sheep were selected as sub-jects in the study ,the exon 3 of Lin28B gene of the selected animals were cloned ,and the real-time PCR technology was used to examine the mRNA expression level of Lin28B gene in the hypothalamus and ovary of juvenile stage and pubertal stage .The results showed the sequences alignment of Lin28B exons 3 of Duo-lang sheep and Hetian sheep were the same ;the expression level of Lin28B gene in the hypothalamus and ovary were significantly lower than that in juvenlie period of Duolang sheep (P< 0 .05) ,the expression lev-el of Lin28B gene in the ovary was significantly lower than that in the juvenlie period of Hetian sheep (P<0 .05) .The results indicated that the down-regulated expression of Lin28B gene was positively correlated with the onset of puberty in sheep .The study can help to provide a scientific basis for revealing the effect and mechanism of Lin28B gene in the onset of puberty in sheep .

  16. Evaluation of the antibacterial potential of Petroselinum crispum and Rosmarinus officinalis against bacteria that cause urinary tract infections.

    Science.gov (United States)

    Petrolini, Fernanda Villas Boas; Lucarini, Rodrigo; de Souza, Maria Gorete Mendes; Pires, Regina Helena; Cunha, Wilson Roberto; Martins, Carlos Henrique Gomes

    2013-01-01

    In this study we evaluated the antibacterial activity of the crude hydroalcoholic extracts, fractions, and compounds of two plant species, namely Rosmarinus officinalis and Petroselinum crispum, against the bacteria that cause urinary tract infection. The microdilution method was used for determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The crude hydroalcoholic extract of R. officinalis displayed in vitro activity against Gram-positive bacteria, with satisfactory MBC for the clinical isolate S. saprophyticus. The fractions and the pure compound rosmarinic acid did not furnish promising results for Gram-negative bacteria, whereas fractions 2, 3, and 4 gave encouraging results for Gram-positive bacteria and acted as bactericide against S. epidermidis as well as E. faecalis (ATCC 29212) and its clinical isolate. R. officinalis led to promising results in the case of Gram-positive bacteria, resulting in a considerable interest in the development of reliable alternatives for the treatment of urinary infections.

  17. Evaluation of the antibacterial potential of Petroselinum crispum and Rosmarinus officinalis against bacteria that cause urinary tract infections

    Directory of Open Access Journals (Sweden)

    Fernanda Villas Boas Petrolini

    2013-09-01

    Full Text Available In this study we evaluated the antibacterial activity of the crude hydroalcoholic extracts, fractions, and compounds of two plant species, namely Rosmarinus officinalis and Petroselinum crispum, against the bacteria that cause urinary tract infection. The microdilution method was used for determination of the minimum inhibitory concentration (MIC and minimum bactericidal concentration (MBC. The crude hydroalcoholic extract of R. officinalis displayed in vitro activity against Gram-positive bacteria, with satisfactory MBC for the clinical isolate S. saprophyticus. The fractions and the pure compound rosmarinic acid did not furnish promising results for Gram-negative bacteria, whereas fractions 2, 3, and 4 gave encouraging results for Gram-positive bacteria and acted as bactericide against S. epidermidis as well as E. faecalis (ATCC 29212 and its clinical isolate. R. officinalis led to promising results in the case of Gram-positive bacteria, resulting in a considerable interest in the development of reliable alternatives for the treatment of urinary infections.

  18. Chemical composition and biological activity of essential oils of Dracocephalum heterophyllum and Hyssopus officinalis from Western Himalaya.

    Science.gov (United States)

    Stappen, Iris; Wanner, Jürgen; Tabanca, Nurhayat; Wedge, David E; Ali, Abbas; Kaul, Vijay K; Lal, Brij; Jaitak, Vikas; Gochev, Velizar K; Schmidt, Erich; Jirovetz, Leopold

    2015-01-01

    The essential oils of two representatives of the Lamiaceae, Dracocephalum heterophyllum Benth. and Hyssopus officinalis L., are described for their antifungal, antibacterial, larvicidal and inect biting deterrent activities. Additionally, the chemical compositions of the essential oils, analyzed by simultaneous GC-MS and GC-FID, and odor descriptions are given. The main components of H. officinalis oil were pinocarvone, cis-pinocamphone, and β-pinene. Citronellol was found as the main compound of D. heterophyllum essential oil. Antibacterial testing by agar dilution assay revealed greater activity of D. heterophyllum against Staphylococcus aureus compared with H. officinalis. D. heterophyllum essential oil also showed promising antifungal activity against Colletotrichum species and was more toxic to Aedes aegypti larvae in a larvicial bioassay. Both essential oils showed high activity in the biting deterrent bioassay.

  19. In Vitro Antimicrobial Activity of Essential Oil of Thymus schimperi, Matricaria chamomilla, Eucalyptus globulus, and Rosmarinus officinalis.

    Science.gov (United States)

    Mekonnen, Awol; Yitayew, Berhanu; Tesema, Alemnesh; Taddese, Solomon

    2016-01-01

    In this study, the in vitro antimicrobial activities of four plant essential oils (T. schimperi, E. globulus, R. officinalis, and M. Chamomilla) were evaluated against bacteria and fungi. The studies were carried out using agar diffusion method for screening the most effective essential oils and agar dilution to determine minimum inhibitory concentration of the essential oils. Results of this study revealed that essential oils of T. schimperi, E. globulus, and R. officinalis were active against bacteria and some fungi. The antimicrobial effect of M. chamomilla was found to be weaker and did not show any antimicrobial activity. The minimum inhibitory concentration values of T. schimperi were chamomilla, T. Schimperi, and R. officinalis. The results indicated that T. schimperi have shown strong antimicrobial activity which could be potential candidates for preparation of antimicrobial drug preparation.

  20. Preliminary assessment of Rosmarinus officinalis toxicity on male Wistar rats' organs and reproductive system Avaliação preliminar da toxicidade de Rosmarinus officinalis no sistema reprodutor e em órgãos de ratos Wistar

    Directory of Open Access Journals (Sweden)

    Rita de Cássia da Silveira e Sá

    2006-09-01

    Full Text Available Rosemary (Rosmarinus officinalis L. - Lamiaceae is a shrub used in the treatment of hepatic, intestinal, renal and respiratory affections. Its toxicity was assessed in female rats and an anti-implantation effect was reported after treatment with this plant. This work analyzes the effect of the short-term administration of R. officinalis extract on vital organs, on the organs of the reproductive system and sperm production of mature male Wistar rats. Adult Wistar rats were treated with 1 mL of R. officinalis aqueous extract at a dose level of 291.2 mg and 582.4 mg/kg of body weight for five days. Body and organs weights, sperm production and food consumption were evaluated. The results showed that the lower dose administration of R. officinalis extract did not significantly alter body and organs weight nor did it interfere with gamete production. However, animals treated with the higher dose showed significant weight increase of the seminal vesicle but no significant alteration of the other variables. Food intake was not affected by the treatments.Alecrim (Rosmarinus officinalis L. - Lamiaceae é uma planta utilizada no tratamento de afecções hepática, intestinal, renal e respiratória. Sua toxicidade foi investigada em ratas e seu efeito antiimplantação foi relatado em estudos prévios. Este trabalho analisa o efeito da administração aguda do extrato de R. officinalis em órgãos vitais, em órgãos do sistema reprodutor e na produção de espermatozóides de ratos Wistar adultos. Ratos Wistar foram tratados com 1 mL do extrato aquoso de R. officinalis na dose de 291,2 mg e 582,4 mg/kg de peso corporal por cinco dias. O peso corporal e de órgãos, a produção de espermatozóides e o consumo de ração foram avaliados. Os resultados mostraram que a administração da dose menor do extrato de R. officinalis não alterou significativamente o peso corporal e de órgãos e nem interferiu com a produção de gametas. Entretanto, os animais

  1. Evaluación de siete poblaciones de espárrago (Asparagus officinalis L. Evaluation of seven asparagus populations (Asparagus officinalis L.

    Directory of Open Access Journals (Sweden)

    ILEANA GATTI

    2000-06-01

    Full Text Available Los materiales de espárrago (Asparagus officinalis L. utilizados hasta el momento por los productores son introducciones realizadas por las casas semilleras y han sido seleccionados para satisfacer requerimientos del mercado de los sitios de origen. El objetivo del siguiente trabajo es evaluar siete poblaciones de espárrago (P1 a P7, con el fin de seleccionar genitores adecuados de manera de recurrir a la hibridación de ellos para obtener materiales adaptados a los requerimientos locales. Las evaluaciones se hicieron sobre plantas individuales, separadas por sexos y manejadas como espárrago blanco, durante los años 1993 y 1994, en el campo experimental de la Facultad de Ciencias Agrarias (Universidad Nacional de Rosario, ubicado en Zavalla, provincia de Santa Fe. La evaluación se realizó sobre planta individual, durante un período de 40 días de cosecha y con los datos obtenidos se realizó un ANOVA y un análisis de agrupamiento. Para elegir progenitores femeninos con altos rendimientos y rendimiento de mercado se determinó que se deberá recurrir a las P1, P2 y P3, las cuales presentan también alto número de turiones. Para altos peso medio y diámetro de turión, así como producción tardía, son indicadas las P5 y P7 como genitores masculinos, teniendo en cuenta que, mientras la P7 presenta bajo rendimiento, la P5 aportaría mejores producciones.The materials of asparagus (Asparagus officinalis L. that growers have in use are imported by seed dealers and have been selected according to overseas markets requirements. With the aim of selecting proper parents to use in a breeding program, seven populations of Asparagus officinalis (P1 to P7, all rised as white asparagus, were tested during 1993 and 1994, at the Experimental Field of the Facultad de Ciencias Agrarias (Universidad Nacional de Rosario placed in Zavalla, Santa Fe, Argentina. The evaluation was made on individual plant in a 40 days of harvest period. ANOVA and a cluster

  2. Characteristics of Aerosol Ionic Compositions in Summer 2003 at Lin'an of Yangtze Delta Region

    Institute of Scientific and Technical Information of China (English)

    YAN Peng; ZHANG Yangmei; YANG Dongzhen; TANG Jie; ZHOU Xiuji

    2006-01-01

    With the size-resolved aerosol mass and ion composition data obtained at Lin'an regional atmospheric pollution monitoring station in July 2003, the size distributions of aerosol mass and ionic components, and the correlations between major ion pairs were analyzed. The primary results indicate that in the period of in-situ measurement, the aerosols are mainly composed of fine particles. The mass of aerosols with size less than 2.1 μm accounts for 66% of the total mass of all size ranges, in which about 50% of the mass is contributed by the particles with size less than 0.65 μm. Similar to the size distributions of aerosol mass,the water-soluble ions are mainly concentrated in the size range of <0.65μm, accounting for about 77% of the sum of analyzed ions, and the ions within the range of <2.1 μm reach 88%. The sulfate, ammonium,and potassium are the dominant ionic components in fine particles (particle size less than 2.1 μm). Ion correlation analysis suggests that the sulfates in fine particles are mostly in the compounds of (NH4)2SO4,Na2SO4, and K2SO4, but for submicron particles the sulfates are mainly in the form of (NH4)2SO4.

  3. OSM-11 facilitates LIN-12 Notch signaling during Caenorhabditis elegans vulval development.

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    Hidetoshi Komatsu

    2008-08-01

    Full Text Available Notch signaling is critical for cell fate decisions during development. Caenorhabditis elegans and vertebrate Notch ligands are more diverse than classical Drosophila Notch ligands, suggesting possible functional complexities. Here, we describe a developmental role in Notch signaling for OSM-11, which has been previously implicated in defecation and osmotic resistance in C. elegans. We find that complete loss of OSM-11 causes defects in vulval precursor cell (VPC fate specification during vulval development consistent with decreased Notch signaling. OSM-11 is a secreted, diffusible protein that, like previously described C. elegans Delta, Serrate, and LAG-2 (DSL ligands, can interact with the lineage defective-12 (LIN-12 Notch receptor extracellular domain. Additionally, OSM-11 and similar C. elegans proteins share a common motif with Notch ligands from other species in a sequence defined here as the Delta and OSM-11 (DOS motif. osm-11 loss-of-function defects in vulval development are exacerbated by loss of other DOS-motif genes or by loss of the Notch ligand DSL-1, suggesting that DOS-motif and DSL proteins act together to activate Notch signaling in vivo. The mammalian DOS-motif protein Deltalike1 (DLK1 can substitute for OSM-11 in C. elegans development, suggesting that DOS-motif function is conserved across species. We hypothesize that C. elegans OSM-11 and homologous proteins act as coactivators for Notch receptors, allowing precise regulation of Notch receptor signaling in developmental programs in both vertebrates and invertebrates.

  4. Emblica Officinalis (Indian Gooseberry Leaves Extract as Corrosion Inhibitor for Mild Steel in 1N HCl Medium

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    R. Saratha

    2010-01-01

    Full Text Available Corrosion inhibition efficiency of acid extract of dry Emblica officinalis leaves for mild steel in 1N HCl medium is investigated in the present study. Experimental methods include weight loss, potentiodynamic polarization and impedance studies. The results indicate Emblica officinalis leaves to be a good corrosion inhibitor of a mixed type and having efficiency of 87.9% at 2% v/v inhibitor concentration. Corrosion inhibition may be due to the spontaneous physical adsorption of the plant constituents on the mild steel surface. Experimental data fitted the Langmuir, Temkin, Freundlich and Flory-Huggins adsorption isotherms.

  5. A role for the malignant brain tumour (MBT domain protein LIN-61 in DNA double-strand break repair by homologous recombination.

    Directory of Open Access Journals (Sweden)

    Nicholas M Johnson

    Full Text Available Malignant brain tumour (MBT domain proteins are transcriptional repressors that function within Polycomb complexes. Some MBT genes are tumour suppressors, but how they prevent tumourigenesis is unknown. The Caenorhabditis elegans MBT protein LIN-61 is a member of the synMuvB chromatin-remodelling proteins that control vulval development. Here we report a new role for LIN-61: it protects the genome by promoting homologous recombination (HR for the repair of DNA double-strand breaks (DSBs. lin-61 mutants manifest numerous problems associated with defective HR in germ and somatic cells but remain proficient in meiotic recombination. They are hypersensitive to ionizing radiation and interstrand crosslinks but not UV light. Using a novel reporter system that monitors repair of a defined DSB in C. elegans somatic cells, we show that LIN-61 contributes to HR. The involvement of this MBT protein in HR raises the possibility that MBT-deficient tumours may also have defective DSB repair.

  6. LIN28B Activation by PRL-3 Promotes Leukemogenesis and a Stem Cell-like Transcriptional Program in AML.

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    Zhou, Jianbiao; Chan, Zit-Liang; Bi, Chonglei; Lu, Xiao; Chong, Phyllis S Y; Chooi, Jing-Yuan; Cheong, Lip-Lee; Liu, Shaw-Cheng; Ching, Ying Qing; Zhou, Yafeng; Osato, Motomi; Tan, Tuan Zea; Ng, Chin Hin; Ng, Siok-Bian; Wang, Shi; Zeng, Qi; Chng, Wee-Joo

    2017-03-01

    PRL-3 (PTP4A3), a metastasis-associated phosphatase, is also upregulated in patients with acute myeloid leukemia (AML) and is associated with poor prognosis, but the underlying molecular mechanism is unknown. Here, constitutive expression of PRL-3 in human AML cells sustains leukemogenesis in vitro and in vivo Furthermore, PRL-3 phosphatase activity dependently upregulates LIN28B, a stem cell reprogramming factor, which in turn represses the let-7 mRNA family, inducing a stem cell-like transcriptional program. Notably, elevated levels of LIN28B protein independently associate with worse survival in AML patients. Thus, these results establish a novel signaling axis involving PRL-3/LIN28B/let-7, which confers stem cell-like properties to leukemia cells that is important for leukemogenesis.Implications: The current study offers a rationale for targeting PRL-3 as a therapeutic approach for a subset of AML patients with poor prognosis. Mol Cancer Res; 15(3); 294-303. ©2016 AACR.

  7. Re-programming of C. elegans male epidermal precursor fates by Wnt, Hox, and LIN-12/Notch activities.

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    Yu, Hui; Seah, Adeline; Sternberg, Paul W

    2010-09-01

    In Caenorhabditiselegans males, different subsets of ventral epidermal precursor (Pn.p) cells adopt distinct fates in a position-specific manner: three posterior cells, P(9-11).p, comprise the hook sensillum competence group (HCG) with three potential fates (1 degrees , 2 degrees , or 3 degrees ), while eight anterior cells, P(1-8).p, fuse with the hyp7 epidermal syncytium. Here we show that activation of the canonical BAR-1 beta-catenin pathway of Wnt signaling alters the competence of P(3-8).p and specifies ectopic HCG-like fates. This fate transformation requires the Hox gene mab-5. In addition, misexpression of mab-5 in P(1-8).p is sufficient to establish HCG competence among these cells, as well as to generate ectopic HCG fates in combination with LIN-12 or EGF signaling. While increased Wnt signaling induces predominantly 1 degrees HCG fates, increased LIN-12 or EGF signaling in combination with MAB-5 overexpression promotes 2 degrees HCG fates in anterior Pn.p cells, suggesting distinctive functions of Wnt, LIN-12, and EGF signaling in specification of HCG fates. Lastly, wild-type mab-5 function is necessary for normal P(9-11).p fate specification, indicating that regulation of ectopic HCG fate formation revealed in anterior Pn.p cells reflect mechanisms of pattern formation during normal hook development.

  8. SynMuv genes redundantly inhibit lin-3/EGF expression to prevent inappropriate vulval induction in C. elegans.

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    Cui, Mingxue; Chen, Jun; Myers, Toshia R; Hwang, Byung Joon; Sternberg, Paul W; Greenwald, Iva; Han, Min

    2006-05-01

    Activation of EGFR-Ras-MAPK signaling in vulval precursor cells (VPCs) by LIN-3/EGF from the gonad induces vulval development in C. elegans. The prevailing view is that LIN-3 overcomes an "inhibitory signal" from the adjacent hyp7 hypodermal syncytium. This view originated from observations indicating that inactivation of functionally redundant Synthetic Multivulva (SynMuv) genes in hyp7 can activate EGFR-Ras-MAPK signaling in the VPCs. Many SynMuv genes encode transcription and chromatin-associated factors, including the Rb ortholog. Here, we show that the SynMuv A and SynMuv B gene classes are functionally redundant for transcriptional repression of the key target gene, lin-3/EGF, in the hypodermis. These observations necessitate a revision of the concept of "inhibitory signaling." They also underscore the importance of preventing inappropriate cell signaling during development and suggest that derepression of growth factors may be the mechanism by which tumor suppressor genes such as Rb can have cell nonautonomous effects.

  9. Properties of the fullerene C60-containing PN Lin49 in the SMC; Explanations of strong near-IR excess

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    Otsuka, Masaaki; Kemper, Francisca; Leal-Ferreira, Marcelo L.; Aleman, Isabel; Bernard-Salas, Jeronimo; Cami, Jan; Ochsendorf, Bram; Peeters, Els

    2016-07-01

    We performed a detailed spectroscopic analysis of the fullerene C6o-containing planetary nebula (PN) Lin49 in the Small Magellanic Cloud (SMC). Lin49 is a C-rich and metal- deficient PN (Z∼⃒0.0006) and its nebular abundances are in agreement with the AGB model for the initially 1.25Mʘ stars with the metallicity Z = 0.001 of Fishlock et al. (2014, [1]). By stellar absorption fitting with TLUSTY, we derived stellar abundances, effective temperature, and surface gravity. We constructed the photo-ionization model with CLOUDY in order to investigate physical conditions of Lin49. The model with the 0.005-0.1 μm radius graphite and a constant hydrogen density shell could not fit the ∼⃒1-5 μm SED owing to the strong near-IR excess. We propose that the near-IR excess indicates (1) the presence of extremely small carbon molecules or (2) the presence of high-density structure surrounding the central star.

  10. Allelopathic activity of micropropagated Hyssopus officinalis L., Lamiaceae, water infusions Atividade alelopática de micropropagado Hyssopus officinalis L. infusãos aquosos

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    Asya P. Dragoeva

    2010-09-01

    Full Text Available The natural habitats of Hyssopus officinalis L. (Lamiaceae in Bulgaria are not sufficient to satisfy the needs of this herb. Micropropagation might be used for obtaining plants with desirable traits. Hyssopus is a medicinal aromatic plant that has not been studied very much. Recently aromatic plants were investigated as potent allelopathic plants. Determining the modes of action of allelochemicals is one of the challenging aspects in allelopathic studies. The objective of this study was to determine the allelopathic activity of micropropagated Hyssopus officinalis L. water infusions. We evaluated the influence of hot and cold infusions made from the aerial parts of hyssopus under laboratory conditions. For this purpose we tested the effects of infusions on germination and root elongation of Cucumis sativus L. and Triticum aestivum L. We determined also the effect on mitotic activity using Allium cepa L. chromosome aberration test. The results demonstrated the presence of water soluble allelopathic compounds in hyssopus tissue. Infusions exhibited growth depressive effect which was stronger at early stage of growth. The inhibitory effects on germination and root elongation were stronger in T. aestivum than in C. sativus. Hyssopus also exerted mitodepressive and genotoxic effect in Allium cepa root tip cells.Os hábitats naturais de Hyssopus officinalis L. (Lamiaceae na Bulgária não são suficientes para satisfazer as necessidades desta erva. A micropropagação poderia ser usada para obter plantas com características desejáveis. Hyssopus é uma planta aromática medicinal que não foi estudada muito. Recentemente plantas aromáticas foram investigadas como potentes plantas alelopáticas. A determinação dos modos da ação de aleloquímicos é um dos aspectos desafiantes em estudos alelopáticos. O objetivo deste estudo foi determinar a atividade alelopática de micropropagado Hyssopus officinalis L. infusãos aquosos. Foi avaliada a influ

  11. Phytochemical analysis and antioxidant activity of Hyssopus officinalis L. from Iran

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    Fatemeh Fathiazad

    2011-12-01

    Full Text Available Introduction: Hyssopus officinalis (L (Hyssop, Family: Lamiaceae, one of the endemic Iranian perennial herb with a long history of medicinal use, was studied to detect some biologically active chemical constituents of the plant. Methods: The flavonoids of the hydromethanolic extract of the aerial parts of Hyssopus officinalis (L. were studied by VLC and crystalisation of the major compound in subsequent fractions. Furthermore, the composition of its essential oil, total phenolic content and antioxidant activities were studied by GC-MS, Folin–Ciocalteau and DPPH reagents respectively. Results: Apigenin 7-O-β-D-glucuronide was isolated as the major flavonoid. All structural elucidation was performed by spectral means. A total of 20 compounds representing 99.97% of the oil have been identified. Myrtenylacetate , Camphor, Germacrene, Spathulenol were the main compounds The total phenol content of the n-butanol and ethylacetate extracts were determined spectrophotometrically according to the Folin–Ciocalteau procedure to be 246 mgGAE g-1 and 51 mgGAE g-1 in the aerial parts of Hyssopus officinalis. The antioxidant activities of apigenin 7-O-β-D-glucuronide, ethylacetate and n-butanol extracts were also determined by DPPH radical scavenging assay with IC50 values of 116×10_3, 103×10_3, 25×10_3 mg mL-1 respectively. The purified flavonoid showed weak radical scavenging activity (IC50 = 116×10_3mg mL_1. N-butanol extract, because of the highest content of total phenolic compounds (246 mgGAE100_1g had the best antioxidant activity (IC50 = 25mg mL_1. Conclusion: On the whole, the findings of the study revealed that Hyssop possesses valuable antioxidant properties for culinary and possible medicinal use.

  12. Salvia officinalis for hot flushes: towards determination of mechanism of activity and active principles.

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    Rahte, Sinikka; Evans, Richard; Eugster, Philippe J; Marcourt, Laurence; Wolfender, Jean-Luc; Kortenkamp, Andreas; Tasdemir, Deniz

    2013-06-01

    Herbal medicinal products are commonly used in alternative treatment of menopausal hot flushes. In a recent clinical study, Salvia officinalis tincture was found to reduce hot flush frequency and intensity. The aim of the current study was the investigation of the mechanism(s) responsible for the anti-hot flush activity of S. officinalis and determination of its active principle(s). The 66% ethanolic tincture, as well as the n-hexane, CHCl₃, and aqueous ethanolic subextracts obtained from the tincture were studied in vitro for two of the most relevant activities, estrogenicity and selective serotonin reuptake inhibition. Because of an increased risk of menopausal women to suffer from Alzheimer's disease, an in vitro acetylcholinesterase inhibition assay was also employed. No activity was observed in the selective serotonin reuptake inhibition or the acetylcholinesterase inhibition assays at the highest test concentrations. The tincture showed no estrogenic effects whereas the aqueous ethanolic subextract exhibited estrogenicity in the ERLUX assay with an EC₅₀ value of 64 µg/mL. Estrogenic activity-guided fractionation of the aqueous ethanolic subextract by a combination of reverse-phase vacuum liquid chromatography and gel chromatography identified luteolin-7-O-glucuronide (EC₅₀ 129 µg/mL) as the active component of the vacuum liquid chromatography fraction 4 (EC₅₀ 69 µg/mL). Luteolin-7-O-glucoside was identified as the putative estrogenic principle of the most potent minor fraction (7.6.7.6, EC₅₀ 0.7 µg/mL) obtained from the initial vacuum liquid chromatography fraction 7 (EC₅₀ 3 µg/mL). This study suggests the involvement of common and ubiquitous estrogenic flavonoids in the anti-hot flush effect of Salvia officinalis, a safe and commonly used herbal medicinal product during the menopause.

  13. The W-shaped pupil in cuttlefish (Sepia officinalis): functions for improving horizontal vision.

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    Mäthger, Lydia M; Hanlon, Roger T; Håkansson, Jonas; Nilsson, Dan-Eric

    2013-05-03

    The eyes of cuttlefish (Sepia officinalis) have a modified horizontal slit-pupil with a distinctive W-shape in bright light, while in darkness the pupil is circular. Two suggestions have previously been made for a function of the W-shape: (1) camouflaging the eye; (2) providing distance information. Since neither of these suggestions can fully explain the function of this pupil across the entire visual field, particularly the frontal and caudal periphery, we re-addressed the question of its functional significance. We took infra-red images of the eyes of live S. officinalis at different light intensities and from different viewing angles. This allowed us to determine the shape and light-admitting area of the pupil for different parts of the visual field. Our data show that the W-shaped pupil projects a blurred "W" directly onto the retina and that it effectively operates as vertical slits for the frontal and caudal parts of the visual field. We also took images of the natural habitat of S. officinalis and calculated the average vertical brightness distribution in the visual habitat. Computing a retinal illumination map shows that the W-shaped pupil is effective in balancing a vertically uneven light field: The constricted pupil reduces light from the dorsal part of the visual field significantly more than it reduces light from the horizontal band. This will cut the amount of direct sunlight that is scattered by the lens and ocular media, and thus improve image contrast particularly for the dimmer parts of the scene. We also conclude that the pupil provides even attenuation along the horizontal band, whereas a circular pupil would attenuate the image relatively more in the important frontal and caudal periphery of the visual field.

  14. Promotion of oogenesis and embryogenesis in the C. elegans gonad by EFL-1/DPL-1 (E2F) does not require LIN-35 (pRB).

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    Chi, Woo; Reinke, Valerie

    2006-08-01

    In Caenorhabditis elegans, EFL-1 (E2F), DPL-1 (DP) and LIN-35 (pRb) act coordinately in somatic tissues to inhibit ectopic cell division, probably by repressing the expression of target genes. EFL-1, DPL-1 and LIN-35 are also present in the germline, but do not always act together. Strong loss-of-function mutations in either efl-1 or dpl-1 cause defects in oogenesis that result in sterility, while lin-35 mutants are fertile with reduced broods. Microarray-based expression profiling of dissected gonads from efl-1, dpl-1 and lin-35 mutants reveals that EFL-1 and DPL-1 promote expression of an extensively overlapping set of target genes, consistent with the expectation that these two proteins function as a heterodimer. Regulatory regions upstream of many of these target genes have a canonical E2F-binding site, suggesting that their regulation by EFL-1/DPL-1 is direct. Many EFL-1/DPL-1 responsive genes encode proteins required for oogenesis and early embryogenesis, rather than cell cycle components. By contrast, LIN-35 appears to function primarily as a repressor of gene expression in the germline, and the genes that it acts on are for the most part distinct from those regulated by EFL-1 and/or DPL-1. Thus, in vivo, C. elegans E2F directly promotes oogenesis and embryogenesis through the activation of a tissue-specific transcriptional program that does not require LIN-35.

  15. Quality control of herbs: determination of amino acids in Althaea officinalis, Matricaria chamomilla and Taraxacum officinale.

    Science.gov (United States)

    Qureshi, Muhammad Nasimullah; Stecher, Guenther; Bonn, Guenther Karl

    2014-05-01

    Analysis of raw materials and final products need reliable methods for the standardization of natural product drugs. Legal guideline also emphasizes on the qualitative and quantitative analyses of the plant constituents in an herbal product. In this study, thin layer chromatography (TLC) and amino acid analyzer was used for the determination of amino acids in plant extracts. Samples for this study were standards and aqueous extracts from Althaea officinalis, Matricaria chamomilla and Taraxacum officinale. Different amino acids in the extracts were detected through TLC. An automatic amino acid analyzer was used for the quantification of amino acids in the plant extracts under study.

  16. Protective effects of Emblica officinalis (Amla) on metal-induced lipid peroxidation in human erythrocytes.

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    Krishnamoorthy, Vijay Kumar; Rather, Irfan Ahmad

    2016-05-01

    The protective potential of Emblica officinalis (amla) was investigated on metal-induced lipid per oxidation in human erythrocytes. Increases in the levels of MDA and catalase activity were assessed as lipid per oxidation. In addition, glutathione peroxidase (GPX), glutathione (GSH), and ascorbic acid levels were assessed as antioxidant indices. Preliminary investigation of the extract exhibited a significant reduction in lipid per oxidation and an increase in antioxidant abilities, such as a decrease in MDA, GPx and GSH (Pamla extract (Pamla extract has significant protective potential against lipid per oxidation.

  17. STUDY OF ANTIMICROBIAL ACTION OF ESSENTIAL OIL FROM STEMS OF ROSMARINUS OFFICINALIS L. (LAMIACEAE

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    Z. M. Tohsirova

    2016-01-01

    Full Text Available The aim of this work was to investigate the antibacterial activities using the method of wells of Rosmarinus officinalis essential oil, introduced in the Botanical garden of PMPI. It is established that the essential oil has the most pronounced antibacterial activities against Staphylococcus aureus and Enterococcus faecalis, less – Escherichia coli and Candida albicans. Antimicrobial effect on the test-cultures of microorganisms Pseudamonas aeruginosa and Proteus vulgaris are not expressed.

  18. Identification of Sensory-Active Phytochemicals in Asparagus (Asparagus officinalis L.).

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    Dawid, Corinna; Hofmann, Thomas

    2012-12-01

    Sensory-directed fractionation of extracts prepared from raw and cooked asparagus (Asparagus officinalis L.), respectively, followed by LC-TOF-MS, LC-MS/MS, and 1D/2D-NMR experiments revealed the chemical structures of nine bitter tasting mono- and bidesmotic saponins as well as the previously not reported 1,2-dithiolan-4-carboxylic acid 6-D-α/β-glucopyranose ester exhibiting an interesting buttery mouth-coating effect. Sensory studies showed that the orosensation imparted by this sulfur compound was reminiscent to that of melting butter and revealed an orosensory recognition threshold of 276.8 μmol/L.

  19. Isoquinoline Alkaloids from Fumaria officinalis L. and Their Biological Activities Related to Alzheimer's Disease.

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    Chlebek, Jakub; Novák, Zdeněk; Kassemová, Dominika; Šafratová, Marcela; Kostelník, Jan; Malý, Lukáš; Ločárek, Miroslav; Opletal, Lubomír; Hošt'álková, Anna; Hrabinová, Martina; Kuneš, Jiří; Novotná, Pavlína; Urbanová, Marie; Nováková, Lucie; Macáková, Kateřina; Hulcová, Daniela; Solich, Petr; Pérez Martín, Concepción; Jun, Daniel; Cahlíková, Lucie

    2016-01-01

    Two new isoquinoline alkaloids, named fumaranine (2) and fumarostrejdine (10), along with 18 known alkaloids were isolated from aerial parts of Fumaria officinalis. The structures of the isolated compounds were elucidated on the basis of spectroscopic analyses and by comparison with literature data. The absolute configuration of the new compound 2 was determined by comparing its circular dichroism spectra with those of known analogs. Compounds isolated in sufficient amounts were evaluated for their acetylcholinesterase, butyrylcholinesterase, prolyl oligopeptidase (POP), and glycogen synthase kinase-3β inhibitory activities. Parfumidine (8) and sinactine (15) exhibited potent POP inhibition activities (IC50 99±5 and 53±2 μM, resp.).

  20. Dalmatian Sage (Salvia officinalis L.: A Review of Biochemical Contents, Medical Properties and Genetic Diversity

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    Martina Grdiša

    2016-01-01

    Full Text Available Dalmatian sage (Salvia officinalis L. represents one of the most significant medicinal autochthonous species in flora of eastern Adriatic coast and islands. It is evergreen outcrossing perennial subshrub with short woody stems that branch extensively and violet flowers. Apart from being native to Mediterranean karst of west Balkan and Apenine peninsula it is cultivated in numerous countries worldwide with Mediterranean and temperate continental climate. From the earliest times it has been used in traditional medicine in healing gingiva, mouth cavity and the sore throat, against bacterial and fungal infections, for wound treatment, memory enhancement, for treating common cold, against sweating, stomach inflammation, ulcer formation, etc. Its essential oil has also been used in preservation of food and as spice as it gives both specific aroma and promotes digestion of food. The essential oil is extremely complex mixture of different active ingredients; however, the thujones and camphor are the dominant compounds and are the parameter by which S. officinalis is distinguished from other Salvia species. The great variability of essential oil composition and yield has been detected depending on various factors such as genotype, environmental conditions, phonological stage, plant parts used for the extraction of essential oil and drying procedure. Molecular genetic analysis of S. officinalis is still limited and comprises the use of RAPD markers, AFLP and SSR markers in assessing mostly the genetic variability and structure of wild S. officinalis populations. Flora. 10.1016/j.flora.2012.06.018 Ivan Sostaric 14.00   Normal 0 21 false false false HR X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso