WorldWideScience

Sample records for calendula officinalis lin

  1. PHYTOCHEMICAL SCREENING OF CALENDULA OFFICINALIS LINN

    Directory of Open Access Journals (Sweden)

    Chakraborthy Guno Sindhu

    2010-09-01

    Full Text Available Identification of primary and secondary constituents has become the utmost important tool for the presence of active moiety. The phytochemical screening of petroleum ether, chloroform, methanol and water extracts of Calendula officinalis leaf done by TLC means. Petroleum ether extract showed the presence of fatty acids, chloroform extracts showed the presence of triterpens and sterols. Flavonoids, carbohydrates, amino acids and saponins were present in methanol extract and saponins, phenolic substances and tannins were present in the water extract of Calendula officinalis.

  2. Seed germination of Calendula officinalis 'Carola' in response to temperature

    Science.gov (United States)

    Calendula (Calendula officinalis 'Carola') is a potential agronomic oilseed crop with application in the paint, coating, and cosmetic industry. Calendula has historically been used for herbal medicinal purposes and an ornamental plant. With the discovery that calendula seeds contain high concentrati...

  3. “IN VITRO” MULTIPLICATION OF CALENDULA OFFICINALIS L.

    OpenAIRE

    Vantu Smaranda

    2015-01-01

    The aim of the present study was to develop a regeneration procedures for Calendula officinalis L., as analternative for biomass production. Calendula officinalis L. (Asteraceae) is an important medicinal plant species withmultitherapeutic, cosmetic, values. Meristematic explants taken from seedlings of Calendula officinalis L. germinated inaseptic conditions were tested for their regenerative potential. The regeneration of whole plants was obtained in twosteps: the shoots were excised and tr...

  4. Diverse Belongings of Calendula officinalis: An Overview

    OpenAIRE

    M U Khan; Ankur Rohilla; Deepika Bhatt; Shadan Afrin; Seema Rohilla; Ansari, S. H.

    2011-01-01

    Herbal drugs therapy is regarded as an important therapy for the treatment of a wide range of diseases. However, enormous improvements have been observed in modern systems of medicine, but Indian herbal plants provide a rich source for health care to prevent different pathological states. Calendula officinalis is an aromatic, erect, annual herb that is indigenous to Europe, cultivated commonly in North America, Balkans, Eastern Europe, Germany and India. Ample studies have reported about the ...

  5. PHYTOCHEMICAL SCREENING OF CALENDULA OFFICINALIS LINN

    OpenAIRE

    Chakraborthy Guno Sindhu

    2010-01-01

    Identification of primary and secondary constituents has become the utmost important tool for the presence of active moiety. The phytochemical screening of petroleum ether, chloroform, methanol and water extracts of Calendula officinalis leaf done by TLC means. Petroleum ether extract showed the presence of fatty acids, chloroform extracts showed the presence of triterpens and sterols. Flavonoids, carbohydrates, amino acids and saponins were present in methanol extract and saponins, phenolic ...

  6. Diverse Belongings of Calendula officinalis: An Overview

    Directory of Open Access Journals (Sweden)

    M. U. Khan

    2011-07-01

    Full Text Available Herbal drugs therapy is regarded as an important therapy for the treatment of a wide range of diseases. However, enormous improvements have been observed in modern systems of medicine, but Indian herbal plants provide a rich source for health care to prevent different pathological states. Calendula officinalis is an aromatic, erect, annual herb that is indigenous to Europe, cultivated commonly in North America, Balkans, Eastern Europe, Germany and India. Ample studies have reported about the anti-inflammatory, antioxidant, antitumour, antigenotoxic, chemoprotective and hepatoprotective potential associated with the drug. The review article describes about the various phytochemical constituents associated with the plant extract. Moreover, numerous pleiotropic effects exhibited by the plant have been clearly discussed.

  7. DNA-based identification of Calendula officinalis (Asteraceae) 1

    OpenAIRE

    Schmiderer, Corinna; Lukas, Brigitte; Ruzicka, Joana; Novak, Johannes

    2015-01-01

    Premise of the study: For the economically important species Calendula officinalis, a fast identification assay based on high-resolution melting curve analysis was designed. This assay was developed to distinguish C. officinalis from other species of the genus and other Asteraceae genera, and to detect C. officinalis as an adulterant of saffron samples. Methods and Results: For this study, five markers (ITS, rbcL, 5′ trnK-matK, psbA-trnH, trnL-trnF) of 10 Calendula species were sequenced and ...

  8. “IN VITRO” MULTIPLICATION OF CALENDULA OFFICINALIS L.

    Directory of Open Access Journals (Sweden)

    Vantu Smaranda

    2015-11-01

    Full Text Available The aim of the present study was to develop a regeneration procedures for Calendula officinalis L., as analternative for biomass production. Calendula officinalis L. (Asteraceae is an important medicinal plant species withmultitherapeutic, cosmetic, values. Meristematic explants taken from seedlings of Calendula officinalis L. germinated inaseptic conditions were tested for their regenerative potential. The regeneration of whole plants was obtained in twosteps: the shoots were excised and transferred to fresh medium and then rooting of these shoots was achieved on the samemedium with 0,02 mg/l benzylaminopurine and 1 mg/l 2,4 dichlorophenoxyacetic acid. The excised shoots weresubcultured for roots induction. Regenerated plants were transferred to ex vitro conditions for an acclimatisation period

  9. Growth and yield response of calendula (Calendula officinalis) to sowing date in the northern U.S.

    Science.gov (United States)

    Calendula (Calendula officinalis L.) seed is a rich source of the conjugated C18:3 fatty acid calendic acid and can serve as a replacement for VOCs in many industrial chemicals such as paints, coatings and adhesives. Calendula is widely adapted to temperate climates and may be a beneficial rotationa...

  10. DNA-based identification of Calendula officinalis (Asteraceae)1

    Science.gov (United States)

    Schmiderer, Corinna; Lukas, Brigitte; Ruzicka, Joana; Novak, Johannes

    2015-01-01

    Premise of the study: For the economically important species Calendula officinalis, a fast identification assay based on high-resolution melting curve analysis was designed. This assay was developed to distinguish C. officinalis from other species of the genus and other Asteraceae genera, and to detect C. officinalis as an adulterant of saffron samples. Methods and Results: For this study, five markers (ITS, rbcL, 5′ trnK-matK, psbA-trnH, trnL-trnF) of 10 Calendula species were sequenced and analyzed for species-specific mutations. With the application of two developed primer pairs located in the trnK 5′ intron and trnL-trnF, C. officinalis could be distinguished from other species of the genus and all outgroup samples tested. Adulterations of Calendula DNA in saffron could be detected down to 0.01%. Conclusions: With the developed assay, C. officinalis can be reliably identified and admixtures of this species as adulterant of saffron can be revealed at low levels. PMID:26649268

  11. Development of tissue culture and virus-induced gene silencing for Calendula officinalis

    OpenAIRE

    LIU, YANBO

    2011-01-01

    Calendula officinalis is grown widely as an ornamental plant across Europe. It belongs to the large. Asteraceae family. In this study, the aim was to explore the possibilities to use Calendula officinalis as a new model organism for flower development and secondary mechanism studies in Asteraceae. Tissue culture of Calendula officinalis was established using nine different cultivars. Murashige & Skoog (MS) medium with four different combinations of plant growth regulators were tested. Of all ...

  12. Assessment of In vitro Sun Protection Factor of Calendula Officinalis L. (Asteraceae) Essential Oil Formulation

    OpenAIRE

    Mishra, AK; Mishra, A.; Chattopadhyay, P.

    2012-01-01

    The present study was undertaken to study the sunscreen activity of herbal formulation. There is no evidence of the sun protection factor (SPF) studies on essential oil of Calendula flowers (Calendula officinalis L., Asteraceae). The study investigates the in vitro SPF by ultraviolet specrtophotometry method of Calendula flower oil in a cream formulation. Calendula oil was isolated by Clavenger's apparatus, compositions were identified by GC–MS and the cream of calendula flower oil was prepar...

  13. Mycoflora of Calendula officinalis L. seeds

    OpenAIRE

    Danuta Pięta

    2013-01-01

    The seeds of Calendula offlcinalis harvested in the years 1985-1987 were investigated. Agar medium with nitrients was used to isolate the fungi. As a result of the mycological analysis, 3642 isolates belonglng to 23 species and dark unsporulating mycelium were obtained. Alternaria alternata dominated among the isolated fungi. Moreover, Botrytis cinerea, Fusarium culmorum, Fusarium avenaceum and Sclerotinia sc1erotiorum were obtained from the seeds.

  14. Solid lipid nanoparticles for delivery of Calendula officinalis extract.

    Science.gov (United States)

    Arana, Lide; Salado, Clarisa; Vega, Sandra; Aizpurua-Olaizola, Oier; de la Arada, Igor; Suarez, Tatiana; Usobiaga, Aresatz; Arrondo, José Luis R; Alonso, Alicia; Goñi, Félix M; Alkorta, Itziar

    2015-11-01

    Solid lipid nanoparticles (SLN) composed of long-chain fatty acids (palmitic acid, stearic acid or arachidic acid), Epikuron 200 (purified phosphatidylcholine), and bile salts (cholate, taurocholate or taurodeoxycholate) have been prepared by dilution of a microemulsion. A total of five different systems were prepared, and characterized by photon correlation spectroscopy, transmission electron microscopy, differential scanning calorimetry, and infrared spectroscopy. The SLN formulation showing optimal properties (lowest size and polydispersity index and highest zeta potential) was obtained with stearic acid and taurodeoxycholate as cosurfactant. This formulation was loaded with Calendula officinalis extract, a natural compound used on ophthalmic formulations given its anti-inflammatory, emollient, and wound repairing activity. Calendula-loaded SLN preparations were characterized in order to determine loading capacity and entrapment efficiency. In vitro cytotoxicity and wound healing efficacy of Calendula-loaded SLN compared to that of a free plant extract were evaluated on a conjunctival epithelium cell line WKD. Our results suggest that this SLN formulation is a safe and solvent-free Calendula extract delivery system which could provide a controlled therapeutic alternative for reducing disease-related symptoms and improving epithelium repair in ocular surface. PMID:26231862

  15. Solid lipid nanoparticles for delivery of Calendula officinalis extract.

    Science.gov (United States)

    Arana, Lide; Salado, Clarisa; Vega, Sandra; Aizpurua-Olaizola, Oier; de la Arada, Igor; Suarez, Tatiana; Usobiaga, Aresatz; Arrondo, José Luis R; Alonso, Alicia; Goñi, Félix M; Alkorta, Itziar

    2015-11-01

    Solid lipid nanoparticles (SLN) composed of long-chain fatty acids (palmitic acid, stearic acid or arachidic acid), Epikuron 200 (purified phosphatidylcholine), and bile salts (cholate, taurocholate or taurodeoxycholate) have been prepared by dilution of a microemulsion. A total of five different systems were prepared, and characterized by photon correlation spectroscopy, transmission electron microscopy, differential scanning calorimetry, and infrared spectroscopy. The SLN formulation showing optimal properties (lowest size and polydispersity index and highest zeta potential) was obtained with stearic acid and taurodeoxycholate as cosurfactant. This formulation was loaded with Calendula officinalis extract, a natural compound used on ophthalmic formulations given its anti-inflammatory, emollient, and wound repairing activity. Calendula-loaded SLN preparations were characterized in order to determine loading capacity and entrapment efficiency. In vitro cytotoxicity and wound healing efficacy of Calendula-loaded SLN compared to that of a free plant extract were evaluated on a conjunctival epithelium cell line WKD. Our results suggest that this SLN formulation is a safe and solvent-free Calendula extract delivery system which could provide a controlled therapeutic alternative for reducing disease-related symptoms and improving epithelium repair in ocular surface.

  16. Calendula officinalis: Central depressive effect and subacute toxicity

    OpenAIRE

    Parente, Leila M. L.; Elson A. Costa; Lécia G. Matos; Paula, José R.; Luiz C. Cunha; Júnior, Geraldo V.; Silveira, Nusa A.

    2009-01-01

    Flores da Calendula officinalis L. (calêndula) vêm sendo utilizadas popularmente como cicatrizante, antiinflamatório e sedativo, entre outras atividades. Os principais componentes químicos encontrados nas flores são óleos essenciais, ácido salicílico, carotenóides, flavonóides, taninos e saponinas triterpênicas. Atividades ansiolítica e analgésica foram relatadas em plantas que apresentam flavonóides em sua composição. Nesse trabalho a atividade do extrato etanólico das flores da ...

  17. Wound healing activity of flower extract of Calendula officinalis.

    Science.gov (United States)

    Preethi, Korengath C; Kuttan, Ramadasan

    2009-01-01

    The effects of oral and topical application of Calendula officinalis flower extract on excision wounds made in rats were checked. The parameters assessed were the days needed for re-epithelization and percentage of wound closure. The hydroxy proline and hexosamine content in the granuloma tissue of the wound was also measured. The percentage of wound closure was 90.0% in the extract-treated group, whereas the control group showed only 51.1% on the eighth day of wounding (p < .01). The days needed for re-epithelization were 17.7 for the control animals; extract treatment at a dose of 20 or 100 mg/kg b.wt reduced the period to 14 and 13 days, respectively. A significant increase was observed in the hydroxy proline and hexosamine content in the extract-treated group compared with the untreated animals. The data indicate potent wound healing activity ofC. officinalis extract. PMID:19601397

  18. Effect of Calendula officinalis cream on achilles tendon healing.

    Science.gov (United States)

    Aro, A A; Perez, M O; Vieira, C P; Esquisatto, M A M; Rodrigues, R A F; Gomes, L; Pimentel, E R

    2015-02-01

    In recent years, the scientific community has undertaken research on plant extracts, searching for compounds with pharmacological activities that can be used in diverse fields of medicine. Calendula officinalis L. is known to have antioxidant, anti-inflammatory, antibacterial, and wound healing properties when used to treat skin burns. Therefore, the purpose of this study was to analyze the effects of C. officinalis on the initial phase of Achilles tendon healing. Wistar rats were separated in three groups: Calendula (Cal)-rats with a transected tendon were treated with topical applications of C. officinalis cream and then euthanized 7 days after injury; Control (C)-rats were treated with only vehicle after transection; and Normal (N)-rats without tenotomy. Higher concentrations of hydroxyproline (an indicator of total collagen) and non-collagenous proteins were observed in the Cal group in relation to the C group. Zymography showed no difference in the amount of the isoforms of metalloproteinase-2 and of metalloproteinase-9, between C and Cal groups. Polarization microscopy images analysis showed that the Cal group presented a slightly higher birefringence compared with the C group. In sections of tendons stained with toluidine blue, the transected groups presented higher metachromasy as compared with the N group. Immunocytochemistry analysis for chondroitin-6-sulfate showed no difference between the C and Cal groups. In conclusion, the topical application of C. officinalis after tendon transection increases the concentrations of collagen and non-collagenous proteins, as well as the collagen organization in the initial phase of healing. PMID:25266273

  19. Topical Calendula officinalis L. successfully treated exfoliative cheilitis: a case report

    OpenAIRE

    Roveroni-Favaretto, Lúcia Helena Denardi; Lodi, Karina Bortolin; Almeida, Janete Dias

    2009-01-01

    Authors describe a case of recurrent exfoliative cheilitis that responded to treatment with a standardized topical preparation of Calendula officinalis L. An eighteen-year-old man was referred to UNESP - São Paulo State University, Department of Biosciences and Oral Diagnosis, São José dos Campos Dental School to investigate a chronic dry scaling lesion on his lips. The patient's main chief was aesthetic compromising. Corticoid therapy was suspended and Calendula officinalis ointment 10% for ...

  20. Evaluation of Calendula officinalis as an anti-plaque and anti-gingivitis agent

    OpenAIRE

    Mayur Sudhakar Khairnar; Babita Pawar; Pramod Parashram Marawar; Ameet Mani

    2013-01-01

    Background: Calendula officinalis (C. officinalis), commonly known as pot marigold, is a medicinal herb with excellent antimicrobial, wound healing, and anti-inflammatory activity. Aim: To evaluate the efficacy of C. officinalis in reducing dental plaque and gingival inflammation. Materials and Methods: Two hundred and forty patients within the age group of 20-40 years were enrolled in this study with their informed consent. Patients having gingivitis (probing depth (PD) ≤3 mm), with a compla...

  1. EVALUATION OF THE EFFICACY OF CALENDULA OFFICINALIS TINCTURE IN SURGICAL WOUND CARE TREATMENT

    OpenAIRE

    Eleonora Canini,; Alessandro Caielli,; Luca Boni; Ilaria Balzelli; Edoardo Raposio; Claudia Porzio,

    2007-01-01

    The aim of this study was to compare wound care treatment with Iodine solution against tincture made of a natural plant, Calendula officinalis. This plant is famous for the anti-flogistic and antibacterial action. We have selected 28 patients, randomizedin two arms of treatment: arm-A (medication with Betadine®); arm B(medication with Calendula officinalis). We have measured wound areas at the 3rd and 15th day of medications by a digital photo system and the software Adobe Photoshop. To evalu...

  2. Atividade antiinflamatória do granulado de Calendula officinalis L. e Matricaria recutita L.

    OpenAIRE

    L.R. Sartori; M. S. Ferreira; F.F. Perazzo; L. Mandalho Lima; J.C.T. Carvalho

    2003-01-01

    Este trabalho descreve os resultados da atividade antiinflamatória de um granulado composto pelos extratos secos de Calendula officinalis L. e Matricaria recutita L. (GECOMR® - granulado encapsulado de Calendula officinalis e Matricaria recutita). Estas espécies são utilizadas na medicina popular devido às suas atividades antiinflamatória, antiúlcera gástrica e antiespasmódica. A redução do processo edematogênico nos grupos de animais tratados com o GECOMR foi observada para todos agentes inf...

  3. Biological Activities of Oleanolic Acid Derivatives from Calendula officinalis Seeds.

    Science.gov (United States)

    Zaki, Ahmed; Ashour, Ahmed; Mira, Amira; Kishikawa, Asuka; Nakagawa, Toshinori; Zhu, Qinchang; Shimizu, Kuniyoshi

    2016-05-01

    Phytochemical examination of butanol fraction of Calendula officinalis seeds led to the isolation of two compounds identified as 28-O-β-D-glucopyranosyl-oleanolic acid 3-O-β-D-glucopyranosyl (1→3)-β-D-glucopyranosiduronic acid (CS1) and oleanolic acid 3-O-β-D-glucopyranosyl (1→3)-β-D-glucopyranosiduronic acid (CS2). Biological evaluation was carried out for these two compounds such as melanin biosynthesis inhibitory, hyaluronic acid production activities, anti obesity using lipase inhibition and adipocyte differentiation as well as evaluation of the protective effect against hydrogen peroxide induced neurotoxicity in neuro-2A cells. The results showed that, compound CS2 has a melanin biosynthesis stimulatory activity; however, compound CS1 has a potent stimulatory effect for the production of hyaluronic acid on normal human dermal fibroblast from adult (NHDF-Ad). Both compounds did not show any inhibitory effect on both lipase and adipocyte differentiation. Compound CS2 could protect neuro-2A cells and increased cell viability against H2 O2 . These activities (melanin biosynthesis stimulatory and protective effect against H2 O2 of CS2 and hyaluronic acid productive activities of these triterpene derivatives) have been reported for the first time. Copyright © 2016 John Wiley & Sons, Ltd. PMID:26887328

  4. Efeito cicatrizante e atividade antibacteriana da Calendula officinalis L. cultivada no Brasil Healing effect and antibacterial activity of Calendula officinalis L. cultivated in Brazil

    OpenAIRE

    L.M.L. Parente; M.S.B. Silva; L.A.B. Brito; R.S. Lino-Júnior; J.R. Paula; L.M.F. Trevenzol; D.T. Zatta; N.M. Paulo

    2009-01-01

    Desde a antiguidade propriedades medicinais são atribuídas às flores da Calendula officinalis L. (Asteraceae) destacando-se a atividade cicatrizante. Estudos sobre a atividade geral de plantas medicinais na cicatrização vêm sendo realizados, sem especificar sobre qual das fases da cicatrização a planta atua. Neste trabalho a atividade cicatrizante e antiinflamatória do extrato etanólico das flores da C. officinalis cultivada no Brasil foi avaliada em feridas cutâneas de ratos Wistar, por meio...

  5. Angiogenic activity of Calendula officinalis flowers L. in rats Atividade angiogênica das flores da Calendula officinalis L. em ratos

    OpenAIRE

    Leila Maria Leal Parente; Maria Auxiliadora Andrade; Luiz Augusto Batista Brito; Veridiana Maria Brianezi Dignani de Moura; Marina Pacheco Miguel; Ruy de Souza Lino-Júnior; Leonice Faustino Manrique Tresvenzol; José Realino de Paula; Neusa Margarida Paulo

    2011-01-01

    Purpose: In this work, angiogenic activity of Calendula officinalis L. (Asteraceae) ethanolic extract and dichloromethane and hexanic fractions were evaluated, considering medicinal properties, especially healing activity, are attributed to this plant. Methods: Models using 36 rats and 90 embryonated eggs were used to evaluate healing and angiogenic activities of extracts and fractions of the plant, through the induction of skin wounds and the chorioallantoic membrane, respectively. The effec...

  6. Antifungal activity of the essential oil from Calendula officinalis L. (asteraceae) growing in Brazil

    OpenAIRE

    Gazim, Zilda Cristiane; Rezende, Claudia Moraes; Fraga, Sandra Regina; Svidzinski, Terezinha Inez Estivaleti; Cortez, Diógenes Aparicio Garcia

    2008-01-01

    This study tested in vitro activity of the essential oil from flowers of Calendula officinalis using disk-diffusion techniques. The antifungal assay results showed for the first time that the essential oil has good potential antifungal activity: it was effective against all 23 clinical fungi strains tested.

  7. Influence of sodium phosphate (E 339) on mitotic division in Calendula officinalis L.

    OpenAIRE

    Romeo-Cristian Marc; Gabriela Capraru

    2008-01-01

    This paper includes the cytogenetic effects induced by sodium phosphate (E 339) food additive in meristematic cells of Calendula officinalis L. root tips. The increase of food additive concentration determined the decrease of mitotic index, while the frequency and the type of chromosome aberrations are much greater in treated variants, comparatively with control.

  8. INFLUENCE OF SODIUM METABISULPHITE (E 223) ON MITOTIC DIVISION IN CALENDULA OFFICINALIS L.s

    OpenAIRE

    Romeo-Cristian Marc; Gabriela Capraru

    2008-01-01

    This paper presents the cytogenetic effects induced by sodium metabisulphite (E 223) (a food additive used as preservative) in meristematic cells of Calendula officinalis L. root tips. The treatment has determined the lessening of the mitotic index (comparative with the control variant), until mitotic division total inhibition, as well as a growth frequency of division aberration in anaphase and telophase.

  9. Bioecological features and corrective properties of Achillea filipendulina Lam., Calendula officinalis L. and Hypericum perforatum L.

    OpenAIRE

    Elshan Shamilov; Asim Abdullayev; Ibragim Azizov; Sitara Mustafaeva; Sevil Zeynalova

    2013-01-01

    The study is devoted to investigations on bioecological features and corrective properties of two species from the family Asteraceae Dumort. (Achillea filipendulina Lam. and Calendula officinalisL.) and one species from the family Hypericaceae Juss. (Hypericum perforatumL.) which are growing in the flora of Azerbaijan. It has revealed that the extract obtained from the collection of these plants shows corrective influence.

  10. Mechanized harvesting and primary processing of Calendula officinalis L. inflorescences

    Directory of Open Access Journals (Sweden)

    Branislav Veselinov

    2014-04-01

    Full Text Available Manual harvesting of marigold (Calendula officinalis L. inflorescences results in superior quality, but requires enormous manpower. The objectives of the research were to examine the possibility of mechanized harvesting of marigold inflorescences by virtual rotating comb-type chamomile harvester, widely used in South Eastern Europe, and to develop the mechanical separator for the inflorescences from the harvested mass. The impact of coefficient R, ratio of the harvester’s penetration into the inflorescences horizon and the width of inflorescences band (i.e. the average value of the highest and lowest inflorescences span on the harvest yield, was tested first. Separation was performed by a bespoke separator that uses five cascades of oscillating sieves made of longitudinally situated rods. Influence of oscillation frequencies and rod distances on separation efficiency was investigated. It was found that, for coefficient R value of 1.3, an average of 97% of the total inflorescences yield can be harvested. The proportion of inflorescences with stem length up to 2 cm was 65% and 35% for stems over 2 cm. The result of mechanized harvest was the reduction in both the number of succeeding harvests and the yield, due to bud damages and elimination. The highest separation efficiency was obtained by using 2.7 Hz oscillation frequency and combination of 6/8 mm rods’ distances in sieves. After three passes of the harvested mass through the device, approximately 92% of inflorescences were separated. Future investigations should be directed towards solving the issue of mechanical shortening of the inflorescences stems and evaluating the economic viability of mechanized harvesting within the proposed procedure.

  11. A new extract of the plant calendula officinalis produces a dual in vitro effect: cytotoxic anti-tumor activity and lymphocyte activation

    OpenAIRE

    Collado Antonia; Algarra Ignacio; Paco Laura; Garcia-Lora Angel; Jiménez-Medina Eva; Garrido Federico

    2006-01-01

    Abstract Background Phytopharmacological studies of different Calendula extracts have shown anti-inflamatory, anti-viral and anti-genotoxic properties of therapeutic interest. In this study, we evaluated the in vitro cytotoxic anti-tumor and immunomodulatory activities and in vivo anti-tumor effect of Laser Activated Calendula Extract (LACE), a novel extract of the plant Calendula Officinalis (Asteraceae). Methods An aqueous extract of Calendula Officinalis was obtained by a novel extraction ...

  12. Dietary Supplementation of Calendula officinalis Counteracts the Oxidative Stress and Liver Damage Resulted from Aflatoxin

    OpenAIRE

    Hamzawy, Mohamed A.; El-Denshary, Ezzeldein S. M.; Hassan, Nabila S.; Fathia A. Mannaa; Abdel-Wahhab, Mosaad A.

    2013-01-01

    This study was conducted to evaluate the total phenolic compounds, the antioxidant properties, and the hepatorenoprotective potential of Calendula officinalis extract against aflatoxins (AFs-) induced liver damage. Six groups of male Sprague-Dawley rats were treated for 6 weeks included the control; the group fed AFs-contaminated diet (2.5 mg/kg diet); the groups treated orally with Calendula extract at low (CA1) and high (CA2) doses (500 and 1000 mg/kg b.w); the groups treated orally with CA...

  13. Efeito cicatrizante e atividade antibacteriana da Calendula officinalis L. cultivada no Brasil Healing effect and antibacterial activity of Calendula officinalis L. cultivated in Brazil

    Directory of Open Access Journals (Sweden)

    L.M.L. Parente

    2009-01-01

    Full Text Available Desde a antiguidade propriedades medicinais são atribuídas às flores da Calendula officinalis L. (Asteraceae destacando-se a atividade cicatrizante. Estudos sobre a atividade geral de plantas medicinais na cicatrização vêm sendo realizados, sem especificar sobre qual das fases da cicatrização a planta atua. Neste trabalho a atividade cicatrizante e antiinflamatória do extrato etanólico das flores da C. officinalis cultivada no Brasil foi avaliada em feridas cutâneas de ratos Wistar, por meio de avaliação macroscópica e histológica. A atividade antimicrobiana do extrato e das frações hexânica e diclorometano também foi avaliada. A atividade antiinflamatória do extrato etanólico da calêndula foi atribuída à diminuição da exsudação serosa, da hiperemia, da deposição de fibrina e da hiperplasia epidermal, além de resultar em crostas mais delgadas e umedecidas. Observaram-se também aumento de colágeno no tecido de granulação e efeito antibacteriano. Assim, o extrato etanólico da calêndula atuou de forma positiva sobre a atividade cicatricial em feridas cutâneas de ratos, bem como apresentou atividade antibacteriana in vitro.Since ancient history medicinal properties are attributed to flowers of Calendula officinalis L. (Asteraceae, mainly concerning its healing activity. Studies about the general activity of medicinal plants in healing wounds have been conducted without specifying in which healing phase the plant acts. In this work, the anti-inflammatory and healing activity of the ethanolic extract of C. officinalis flowers cultured in Brazil was evaluated in cutaneous wounds of Wistar rats through macroscopic and histological evaluation. The antibacterial activity of the extract and dichloromethane and hexane fractions was also evaluated. The anti-inflammatory activity of calendula ethanolic extract led to a reduction in plasmatic exudation, hyperemia, fibrin deposition and epidermal hyperplasia, besides

  14. Bioecological features and corrective properties of Achillea filipendulina Lam., Calendula officinalis L. and Hypericum perforatum L.

    Directory of Open Access Journals (Sweden)

    Elshan Shamilov

    2013-04-01

    Full Text Available The study is devoted to investigations on bioecological features and corrective properties of two species from the family Asteraceae Dumort. (Achillea filipendulina Lam. and Calendula officinalisL. and one species from the family Hypericaceae Juss. (Hypericum perforatumL. which are growing in the flora of Azerbaijan. It has revealed that the extract obtained from the collection of these plants shows corrective influence.

  15. Componential Profile and Amylase Inhibiting Activity of Phenolic Compounds from Calendula officinalis L. Leaves

    OpenAIRE

    Olennikov, Daniil N.; Nina I. Kashchenko

    2014-01-01

    An ethanolic extract and its ethyl acetate-soluble fraction from leaves of Calendula officinalis L. (Asteraceae) were found to show an inhibitory effect on amylase. From the crude extract fractions, one new phenolic acid glucoside, 6′-O-vanilloyl- β -D-glucopyranose, was isolated, together with twenty-four known compounds including five phenolic acid glucosides, five phenylpropanoids, five coumarins, and nine flavonoids. Their structures were elucidated based on chemical and spectral data. Th...

  16. Componential Profile and Amylase Inhibiting Activity of Phenolic Compounds from Calendula officinalis L. Leaves

    OpenAIRE

    Olennikov, Daniil N.; Nina I. Kashchenko

    2014-01-01

    An ethanolic extract and its ethyl acetate-soluble fraction from leaves of Calendula officinalis L. (Asteraceae) were found to show an inhibitory effect on amylase. From the crude extract fractions, one new phenolic acid glucoside, 6′-O-vanilloyl-β-D-glucopyranose, was isolated, together with twenty-four known compounds including five phenolic acid glucosides, five phenylpropanoids, five coumarins, and nine flavonoids. Their structures were elucidated based on chemical and spectral data. The ...

  17. The effects of extraction method on recovery rutin from Calendula officinalis L. (Asteraceae)

    OpenAIRE

    Frederico Severino Martins; Edemilson Cardoso da Conceição; Elane Sousa Bandeira; José Carréra Silva; Roseane Maria Ribeiro Costa

    2014-01-01

    Background: Calendula officinalis L. (Asteraceae) is a Mediterranean specie, but in Europe and America it is cultivated for ornamental or medicinal purposes. This species is widely used for presenting activities, antiinflammatory antibacterial and antioxidant. However the therapeutic action is linked to the amount of assets of the extracted raw material. The extraction method of bioactive compounds is an important step in the manufacturing of herbal medicines, because secondary metabolites wi...

  18. Production of Sterilizing Agents from Calendula officinalis Extracts Optimized by Response Surface Methodology

    OpenAIRE

    Fatih Mehmet Goktas; Bilgesu Sahin; Sibel Yigitarslan

    2015-01-01

    The aim of this study was to produce hand sterilizing liquid and wet wipes with the extracts of Calendula officinalis. Since this plant has well known antimicrobial activity due to its phytochemical constituents, the increase in the extraction yield was chosen as the principle part of the production process. To achieve the maximum yield, parameters of solid-to-liquid ratio, extraction temperature, and time were studied. The optimum conditions were determined by response surface methodology as...

  19. PARAMETERS OF THE IMMUNOLOGICAL PROFILE IN CHICKENS TREATED WITH A CALENDULA OFFICINALIS EXTRACTION

    OpenAIRE

    Dumitru, C.; SPÎNU, M.; G BRUDAOCĂ; DOBREAN, V.; A OPRIO; ANDRU, C.

    2002-01-01

    Tests were carried out on thirty, 42 days old Rock x Cornish chickens, divided into three experimental groups: I - control injected with saline, II - injected with a Calendula officinalis extraction supplemented with Bayol and III - treated with Bayol. The effects of the in vivo treatments and simultaneous antigen priming (0.5 ml of a 5 % suspension of SRBC) on their humoral (lysozyme, anti-SRBC antibodies) and cell-mediated (leucocyte blast transformation) responses were investigated. Lysozy...

  20. Healing Acceleration of Acetic Acid-induced Colitis by Marigold (Calendula officinalis) in Male Rats

    OpenAIRE

    Nader Tanideh; Akram Jamshidzadeh; Masood Sepehrimanesh; Masood Hosseinzadeh; Omid Koohi-Hosseinabadi; Asma Najibi; Mozhdeh Raam; Sajad Daneshi; Seyedeh-Leili Asadi-Yousefabad

    2016-01-01

    Background/Aim: Ulcerative colitis (UC) is a type of chronic inflammatory bowel disease with unknown etiology. Several therapeutic strategies such as consumption of medicinal plants have been used for its treatment. The aim of this study was to evaluate healing effects of Calendula officinalis hydroalcoholic extract in experimentally induced UC in rat. Materials and Methods: Ninety-six rats, weighing 200 ± 20 g, were randomly divided into eight equal groups. UC induced by 3% acetic acid and o...

  1. ANTIDIABETIC AND ANTIHYPERLIPIDAEMIC EFFECT OF HYDRO-ALCOHOLIC EXTRACT OF CALENDULA OFFICINALIS

    OpenAIRE

    Chakraborthy G.S; Arora R; Majee C

    2011-01-01

    Calendula officinalis, belonging to the family of Asteraceae, commonly known as English Marigold or Pot Marigold is an aromatic herb which is used in Traditional System of Medicine. It is mainly used because of its various biological activities to treat diseases like analgesic, antidiabetic and anti-inflammatory. It is also used for ingastro-intestinal, gynecological, eye disease, skin injuries and in some cases of burn. The plant is rich in many pharmaceutical active ingredients like Caroten...

  2. Fatty acid composition of lipids in pot marigold (Calendula officinalis L.) seed genotypes

    OpenAIRE

    Dulf Francisc V; Pamfil Doru; Baciu Adriana D; Pintea Adela

    2013-01-01

    Abstract Background Calendula officinalis L. (pot marigold) is an annual aromatic herb with yellow or golden-orange flowers, native to the Mediterranean climate areas. Their seeds contain significant amounts of oil (around 20%), of which about 60% is calendic acid. For these reasons, in Europe concentrated research efforts have been directed towards the development of pot marigold as an oilseed crop for industrial purposes. Results The oil content and fatty acid composition of major lipid fra...

  3. Biocontrol and Plant Growth Promotion Characterization of Bacillus Species Isolated from Calendula officinalis Rhizosphere

    OpenAIRE

    Ait Kaki, A.; Kacem Chaouche, N.; Dehimat, L.; Milet, A.; Youcef-Ali, M.; Ongena, Marc; Thonart, Philippe

    2013-01-01

    The phenotypic and genotypic diversity of the plant growth promoting Bacillus genus have been widely investigated in the rhizosphere of various agricultural crops. However, to our knowledge this is the first report on the Bacillus species isolated from the rhizosphere of Calendula officinalis. 15 % of the isolated bacteria were screened for their important antifungal activity against Fusarium oxysporum, Botrytis cinerea, Aspergillus niger, Cladosporium cucumerinium and Alternaria alternata. T...

  4. Carotenoid isomerase is key determinant of petal color of Calendula officinalis.

    Science.gov (United States)

    Kishimoto, Sanae; Ohmiya, Akemi

    2012-01-01

    Orange petals of calendula (Calendula officinalis) accumulate red carotenoids with the cis-configuration at the C-5 or C-5' position (5-cis-carotenoids). We speculated that the orange-flowered calendula is a carotenoid isomerase (crtiso) loss-of-function mutant that impairs the cis-to-trans conversion of 5-cis-carotenoids. We compared the sequences and enzyme activities of CRTISO from orange- and yellow-flowered calendulas. Four types of CRTISO were expressed in calendula petals. The deduced amino acid sequence of one of these genes (CoCRTISO1) was different between orange- and yellow-flowered calendulas, whereas the sequences of the other three CRTISOs were identical between these plants. Analysis of the enzymatic activities of the CoCRTISO homologs showed that CoCRTISO1-Y, which was expressed in yellow petals, converted carotenoids from the cis-to-trans-configuration, whereas both CoCRTISO1-ORa and 1-ORb, which were expressed in orange petals, showed no activity with any of the cis-carotenoids we tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines linked closely to petal color. These data indicate that CoCRTISO1 is a key regulator of the accumulation of 5-cis-carotenoids in calendula petals. Site-directed mutagenesis showed that the deletion of Cys-His-His at positions 462-464 in CoCRTISO1-ORa and a Gly-to-Glu amino acid substitution at position 450 in CoCRTISO1-ORb abolished enzyme activity completely, indicating that these amino acid residues are important for the enzymatic activity of CRTISO. PMID:22069331

  5. A Randomized Comparative Trial on the Therapeutic Efficacy of Topical Aloe vera and Calendula officinalis on Diaper Dermatitis in Children

    OpenAIRE

    Yunes Panahi; Mohamad Reza Sharif; Alireza Sharif; Fatemeh Beiraghdar; Zahra Zahiri; Golnoush Amirchoopani; Eisa Tahmasbpour Marzony; Amirhossein Sahebkar

    2012-01-01

    Introduction. Diaper dermatitis (DD) is a common inflammatory disorder among children and infants. The objective of the present randomized and double-blind trial was to compare the therapeutic efficacies of Aloe vera cream and Calendula officinalis ointment on the frequency and severity of DD in children. Methods. Sixty-six infants with DD (aged < 3 years) were randomized to receive either Aloe cream (n = 32) or Calendula ointment (n = 34). Infants were treated with these drugs 3 times a day ...

  6. Usage of Calendula officinalis in the prevention and treatment of radiodermatitis: a randomized double-blind controlled clinical trial.

    Science.gov (United States)

    Schneider, Franciane; Danski, Mitzy Tannia Reichembach; Vayego, Stela Adami

    2015-01-01

    OBJECTIVE To evaluate the efficacy of Calendula officinalis in relation to Essential Fatty Acids for the prevention and treatment of radiodermatitis. METHOD This is a randomized double-blind controlled clinical trial with 51 patients with head and neck cancer in radiotherapy treatment divided into two groups: control (27) and experimental (24). RESULTS There is statistically significant evidence (p-value = 0.0120) that the proportion of radiodermatitis grade 2 in Essential Fatty Acids group is higher than Calendula group. Through the Kaplan-Meier survival curve we observed that Essential Fatty Acids group has always remained below the Calendula group survival curve, due to the lower risk of developing radiodermatitis grade 1, which makes the usage of Calendula more effective, with statistical significance (p-value = 0.00402). CONCLUSION Calendula showed better therapeutic response than the Essential Fatty Acids in the prevention and treatment of radiodermatitis. Brazilian Registry of Clinical Trials: RBR-237v4b. PMID:25992820

  7. Usage of Calendula officinalis in the prevention and treatment of radiodermatitis: a randomized double-blind controlled clinical trial

    Directory of Open Access Journals (Sweden)

    Franciane Schneider

    2015-04-01

    Full Text Available OBJECTIVE To evaluate the efficacy of Calendula officinalis in relation to Essential Fatty Acids for the prevention and treatment of radiodermatitis. METHOD This is a randomized double-blind controlled clinical trial with 51 patients with head and neck cancer in radiotherapy treatment divided into two groups: control (27 and experimental (24. RESULTS There is statistically significant evidence (p-value = 0.0120 that the proportion of radiodermatitis grade 2 in Essential Fatty Acids group is higher than Calendula group. Through the Kaplan-Meier survival curve we observed that Essential Fatty Acids group has always remained below the Calendula group survival curve, due to the lower risk of developing radiodermatitis grade 1, which makes the usage of Calendula more effective, with statistical significance (p-value = 0.00402. CONCLUSION Calendula showed better therapeutic response than the Essential Fatty Acids in the prevention and treatment of radiodermatitis. Brazilian Registry of Clinical Trials: RBR-237v4b.

  8. Antifungal activity of the essential oil from Calendula officinalis L. (asteraceae) growing in Brazil Atividade antifúngica do óleo essencial da Calendula officinalis cultivada no Brasil

    OpenAIRE

    Zilda Cristiane Gazim; Claudia Moraes Rezende; Sandra Regina Fraga; Terezinha Inez Estivaleti Svidzinski; Diógenes Aparicio Garcia Cortez

    2008-01-01

    This study tested in vitro activity of the essential oil from flowers of Calendula officinalis using disk-diffusion techniques. The antifungal assay results showed for the first time that the essential oil has good potential antifungal activity: it was effective against all 23 clinical fungi strains tested.Neste estudo foi avaliada a atividade antifúngica do óleo essencial, obtido das flores de Calendula officinalis utilizando-se técnica de difusão em discos. Os resultados dos ensaios antifún...

  9. Dietary Supplementation of Calendula officinalis Counteracts the Oxidative Stress and Liver Damage Resulted from Aflatoxin.

    Science.gov (United States)

    Hamzawy, Mohamed A; El-Denshary, Ezzeldein S M; Hassan, Nabila S; Mannaa, Fathia A; Abdel-Wahhab, Mosaad A

    2013-01-01

    This study was conducted to evaluate the total phenolic compounds, the antioxidant properties, and the hepatorenoprotective potential of Calendula officinalis extract against aflatoxins (AFs-) induced liver damage. Six groups of male Sprague-Dawley rats were treated for 6 weeks included the control; the group fed AFs-contaminated diet (2.5 mg/kg diet); the groups treated orally with Calendula extract at low (CA1) and high (CA2) doses (500 and 1000 mg/kg b.w); the groups treated orally with CA1 and CA2 one week before and during AFs treatment for other five weeks. The results showed that the ethanol extract contained higher phenolic compounds and posses higher 1,1-diphenyl 1-2-picryl hydrazyl (DPPH) radical scavenging activity than the aqueous extract. Animals fed AFs-contaminated diet showed significant disturbances in serum biochemical parameters, inflammatory cytokines, and the histological and histochemical pictures of the liver accompanied by a significant increase in malondialdehyde (MDA) and a significant decrease in superoxide dismutase (SOD) and glutathione peroxidase (GPx) in liver. Calendula extract succeeded to improve the biochemical parameters, inflammatory cytokines, decreased the oxidative stress, and improved the histological pictures in the liver of rats fed AFs-contaminated diet in a dose-dependent manner. It could be concluded that Calendula extract has potential hepatoprotective effects against AFs due to its antioxidant properties and radical scavenging activity. PMID:24959547

  10. Effect of Semiarid Environment on Some Nutritional and Antinutritional Attributes of Calendula (Calendula officinalis)

    OpenAIRE

    Muhammad Iqbal; Iqbal Hussain; Asma Habib; Muhammad Arslan Ashraf; Rizwan Rasheed

    2015-01-01

    Stressful environments have been shown to affect the metabolism in some plants. In the present study, we assessed whether semiarid environment (saline and saline alkaline soil) could affect the nutritional (total proteins, phenolics and riboflavin contents, and catalase activity) and antinutritional (hydrogen peroxide and malondialdehyde contents) properties differently in different plant parts (leaves, flowers, and roots) of calendula. Although salinity decreased plant biomass, it did not af...

  11. Effect of water regime on the growth, flower yield, essential oil and proline contents of Calendula officinalis

    OpenAIRE

    SAMI ALI METWALLY; KHALID ALI KHALID; BEDOUR H. ABOU-LEILA

    2013-01-01

    Metwally SA,Khalid KA, Abou-Leila BH. 2013. Effect of water regime on the growth, flower yield, essential oil and proline contents of Calendula officinalis. Nusantara Bioscience 5: 63-67. The effects of water regime on the growth, content of essential oil and proline of Calendula officinalis L. plants were investigated. Water regimes of 75% of field water capacity increased certain growth characters [i.e. plant height (cm), leaf area (cm2), flower diameter (cm) and spike stem diameter] and va...

  12. Actividad cicatrizante y ensayos de irritación de la crema de Calendula officinalis al 1%

    OpenAIRE

    Menéndez, Addis Bellma; Lagarto Parra, Alicia; Bueno Pavón, Viviana; Carrillo Domínguez, Carmen; Valdés Martínez, Odalys; Guerra Sardiñas, Isabel; Muñoz, Adriana

    2007-01-01

    El proceso de cicatrización de una herida en la piel involucra la compleja interacción de mu- chos tipos de células y ocurre como una cascada secuencial de procesos solapados e íntimamente relaciona- dos. A Calendula officinalis L. se le atribuyen efectos curativos sobre las lesiones de la piel como cicatrizante, por lo que se estudió el efecto cicatrizante e irritante ocular dérmico y oftálmico de una crema al 1% de Calendula officinalis L. sobre la cicatrización de heridas abiertas en piel ...

  13. Effect of water regime on the growth, flower yield, essential oil and proline contents of Calendula officinalis

    Directory of Open Access Journals (Sweden)

    SAMI ALI METWALLY

    2013-11-01

    Full Text Available Metwally SA,Khalid KA, Abou-Leila BH. 2013. Effect of water regime on the growth, flower yield, essential oil and proline contents of Calendula officinalis. Nusantara Bioscience 5: 63-67. The effects of water regime on the growth, content of essential oil and proline of Calendula officinalis L. plants were investigated. Water regimes of 75% of field water capacity increased certain growth characters [i.e. plant height (cm, leaf area (cm2, flower diameter (cm and spike stem diameter] and vase life (day. Water regime promoted the accumulation of essential oil content and its main components as well as proline contents.

  14. Effect of Semiarid Environment on Some Nutritional and Antinutritional Attributes of Calendula (Calendula officinalis

    Directory of Open Access Journals (Sweden)

    Muhammad Iqbal

    2015-01-01

    Full Text Available Stressful environments have been shown to affect the metabolism in some plants. In the present study, we assessed whether semiarid environment (saline and saline alkaline soil could affect the nutritional (total proteins, phenolics and riboflavin contents, and catalase activity and antinutritional (hydrogen peroxide and malondialdehyde contents properties differently in different plant parts (leaves, flowers, and roots of calendula. Although salinity decreased plant biomass, it did not affect total protein and phenolics contents in the calendula. All plant parts were rich in riboflavin contents. However, plants grown under saline-alkali soil had relatively more riboflavin contents in the flowers. Salinity increased hydrogen peroxide (H2O2 concentration in the flowers and roots, whereas saline-alkali soil did not affect it. Plants exposed to both saline and saline-alkali soil had greater catalase activity in the flowers and leaves. Plants exposed to salinity had higher malondialdehyde (MDA contents in the flowers compared with nonsaline and saline-alkali conditions. Nonetheless, the possibility of safely using different parts of calendula as nutraceutical was in the order flower > root > leaf. Overall, the results suggested that plant can be grown in mild saline-alkali (EC ≤ 7; pH = 8.5 soils without affecting its nutraceutical properties.

  15. Angiogenic activity of Calendula officinalis flowers L. in rats Atividade angiogênica das flores da Calendula officinalis L. em ratos

    Directory of Open Access Journals (Sweden)

    Leila Maria Leal Parente

    2011-02-01

    Full Text Available Purpose: In this work, angiogenic activity of Calendula officinalis L. (Asteraceae ethanolic extract and dichloromethane and hexanic fractions were evaluated, considering medicinal properties, especially healing activity, are attributed to this plant. Methods: Models using 36 rats and 90 embryonated eggs were used to evaluate healing and angiogenic activities of extracts and fractions of the plant, through the induction of skin wounds and the chorioallantoic membrane, respectively. The effect of vascular proliferation was also tested from the study to verify the intensity of expression of vascular endothelial growth factor (VEGF in cutaneous wounds in rats. Results: The angiogenic activity of the extract and the fractions was evidenced in both experimental models. It was verified that this effect is not directly related to the expression of VEGF and it could be associated to other pro-angiogenic factors. Conclusion: The healing activity referred to C. officinalis is related, among other factors, to its positive effect on angiogenesis, characterized by the induction of neovascularization.Objetivo: Neste trabalho a atividade sobre a angiogênese do extrato etanólico (EEC e das frações diclorometano e hexânica das flores de Calendula officinalis L. (Asteraceae cultivada no Brasil foram avaliados, visto que propriedades medicinais têm sido atribuídas às flores da planta, destacando-se a atividade cicatrizante. Métodos: Modelos utilizando 36 ratos e 90 ovos embrionados foram usados para avaliar as atividades cicatrizante e angiogênica dos extratos e frações da planta, por meio da indução de feridas cutâneas e da membrana corioalantóide, respectivamente. O efeito proliferativo vascular foi também testado a partir do estudo imunoistoquímico, realizado para verificar a intensidade da expressão do fator de crescimento endotelial vascular (VEGF na derme de ratos. Resultados: A atividade angiogênica do extrato e das frações foi

  16. Response of Calendula officinalis L. to long-term fumigation with SO2

    Energy Technology Data Exchange (ETDEWEB)

    Singh, S.N.; Yunus, M.; Srivastava, K.; Kulshreshtha, K.; Ahmad, K.J.

    1985-01-01

    Seedlings of Calendula officinalis L. (30-days old) were raised in 1 mS plots and fumigated with 1 and 2 g liter SO2 on alternate days. Analysis of plant samples collected at pre-flowering, flowering and post-flowering stages showed that photosynthetic pigments were degraded and leaf extract pH and protein content declined in SO2 treated plants. In addition, scanning electron microscopic studies revealed that SO2 stimulated the widening of stomatal pores in fumigated plants.

  17. Componential profile and amylase inhibiting activity of phenolic compounds from Calendula officinalis L. leaves.

    Science.gov (United States)

    Olennikov, Daniil N; Kashchenko, Nina I

    2014-01-01

    An ethanolic extract and its ethyl acetate-soluble fraction from leaves of Calendula officinalis L. (Asteraceae) were found to show an inhibitory effect on amylase. From the crude extract fractions, one new phenolic acid glucoside, 6'-O-vanilloyl-β-D-glucopyranose, was isolated, together with twenty-four known compounds including five phenolic acid glucosides, five phenylpropanoids, five coumarins, and nine flavonoids. Their structures were elucidated based on chemical and spectral data. The main components, isoquercitrin, isorhamnetin-3-O-β-D-glucopyranoside, 3,5-di-O-caffeoylquinic acid, and quercetin-3-O-(6''-acetyl)-β-D-glucopyranoside, exhibited potent inhibitory effects on amylase. PMID:24683352

  18. Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes

    OpenAIRE

    Palacios, Alejandro; Barberón, Javier; Leaden, Patricio; Zeinsteger, Pedro

    2016-01-01

    In this study the effects of Calendula officinalis L. (Asteraceae) extract (CO) on the polyunsaturated fatty acid composition, chemiluminescence and unsaturation index of microsomes isolated from brain rat, are presented. After incubation of microsomes in an ascorbate (0.4 mM)-Fe2+ (2.15 μM) system (180 min at 37 °C) it was observed that the total cpm/mg protein originated from light emission:chemiluminescence was lower in brain microsomes obtained from CO group compared to the control group ...

  19. Controversial effects of Calendula officinalis L. on Biochemical and Pathological Factors of Nephropathy in Diabetic Wistar Rats

    OpenAIRE

    Salehi; Moradkhani; Mohammadi Roushandeh; Nazari; Pouyandeh Ravan

    2015-01-01

    Background Chronic hyperglycemia leads to microvascular and macrovascular complications such as diabetic nephropathy. Medicinal plants are good sources for finding new therapeutic chemicals to improve diabetes and relieve its symptoms. Objectives The purpose of the present study was to evaluate the effect of the hydroalcoholic extract (300 mg/kg) of Calendula officinalis (marigold) on blood biochemical profiles and histopathologic...

  20. Phenotypic and biochemical profile changes in calendula (Calendula officinalis L.) plants treated with two chemical mutagenesis.

    Science.gov (United States)

    El-Nashar, Y I; Asrar, A A

    2016-01-01

    Chemical mutagenesis is an efficient tool used in mutation-breeding programs to improve the vital characters of the floricultural crops. This study aimed to estimate the effects of different concentrations of two chemical mutagens; sodium azide (SA) and diethyl sulfate (DES). The vegetative growth and flowering characteristics in two generations (M1 and M2) of calendula plants were investigated. Seeds were treated with five different concentrations of SA and DES (at the same rates) of 1000, 2000, 3000, 4000, and 5000 ppm, in addition to a control treatment of 0 ppm. Results showed that lower concentrations of SA mutagen had significant effects on seed germination percentage, plant height, leaf area, plant fresh weight, flowering date, inflorescence diameter, and gas-exchange measurements in plants of both generations. Calendula plants tended to flower earlier under low mutagen concentrations (1000 ppm), whereas higher concentrations delayed flowering significantly. Positive results on seed germination, plant height, number of branches, plant fresh weight, and leaf area were observed in the M2-generation at lower concentrations of SA (1000 ppm), as well as at 4000 ppm DES on number of leaves and inflorescences. The highest total soluble protein was detected at the concentrations of 1000 ppm SA and 2000 ppm DES. DES showed higher average of acid phosphatase activity than SA. Results indicated that lower concentrations of SA and DES mutagens had positive effects on seed germination percentage, plant height, leaf area, plant fresh weight, flowering date, inflorescence diameter, and gas-exchange measurements. Thus, lower mutagen concentrations could be recommended for better floral and physio-chemical performance. PMID:27173326

  1. Production of Sterilizing Agents from Calendula officinalis Extracts Optimized by Response Surface Methodology.

    Science.gov (United States)

    Goktas, Fatih Mehmet; Sahin, Bilgesu; Yigitarslan, Sibel

    2015-01-01

    The aim of this study was to produce hand sterilizing liquid and wet wipes with the extracts of Calendula officinalis. Since this plant has well known antimicrobial activity due to its phytochemical constituents, the increase in the extraction yield was chosen as the principle part of the production process. To achieve the maximum yield, parameters of solid-to-liquid ratio, extraction temperature, and time were studied. The optimum conditions were determined by response surface methodology as 41°C, 7 h, and 3.3 g/200 mL for temperature, time, and solid-to-liquid ratio, respectively. The yield achieved at those conditions was found to be 90 percent. The highest amounts of flavonoids were detected at optimum, whereas the highest triterpene and saponin constituents were determined at different design points. The microbial efficiencies of extracts were determined by the inhibition of the growth of selected microorganisms. Different dilution rates and interaction times were used as parameters of inhibition. Not any of the constituent but symbiotic relation in-between reached the highest inhibition of 90 percent. The pH values of the extracts were 5.1 to 5.4. As a result, the extraction of Calendula officinalis at the optimum conditions can be used effectively in the production of wet wipes and hand sterilizing liquid. PMID:26064122

  2. Phytochemical evaluation and in vitro antioxidant and photo-protective capacity of Calendula officinalis L. leaves

    Directory of Open Access Journals (Sweden)

    V.C.K.N. DEUSCHLE

    2015-01-01

    Full Text Available ABSTRACT The plant Calendula officinalis L. is widely applied due to its medicinal properties, which are mainly dermatological and ornamental. The goal of this study is to assess the phytochemical components in a hydroethanolic extract (HECO from the leaves of Calendula officinalis L. using UV-VIS spectrophotometry and thin layer chromatography (TLC, as well as to identify and quantify the components related to its antioxidant capacity employing high performance liquid chromatographic (HPLC. The antioxidant capacity evaluation was performed using the DPPH method for superoxide and hydroxyl radicals. The photo-protective capacity was evaluated by UVspectrophotometry in order to determine the in vitro Sun Protection Factor(SPF. The results show the plant’s strong antioxidant activity (DPPH and hydroxyl methods, which we believe to be related to the presence of flavonoids (24.67 mg/g, polyphenols (33.90 mg/g, condensed tannins (27.30 mg/g, and the amount of rutin (37.25 mg/g, and quercetin (6.09 mg/g found during the study. The HECO presented a good antioxidant capacity, most likely due to the polyphenols, flavonoids, and tannins in its contents. However, the obtained SPF of 1.89 ± 0.05 does not allow the plant to be classified as a stand-alone sunscreen, and more studies are needed in order to test its ability to enhance sunscreens in existing cosmetic formulations.

  3. Production of Sterilizing Agents from Calendula officinalis Extracts Optimized by Response Surface Methodology

    Directory of Open Access Journals (Sweden)

    Fatih Mehmet Goktas

    2015-01-01

    Full Text Available The aim of this study was to produce hand sterilizing liquid and wet wipes with the extracts of Calendula officinalis. Since this plant has well known antimicrobial activity due to its phytochemical constituents, the increase in the extraction yield was chosen as the principle part of the production process. To achieve the maximum yield, parameters of solid-to-liquid ratio, extraction temperature, and time were studied. The optimum conditions were determined by response surface methodology as 41°C, 7 h, and 3.3 g/200 mL for temperature, time, and solid-to-liquid ratio, respectively. The yield achieved at those conditions was found to be 90 percent. The highest amounts of flavonoids were detected at optimum, whereas the highest triterpene and saponin constituents were determined at different design points. The microbial efficiencies of extracts were determined by the inhibition of the growth of selected microorganisms. Different dilution rates and interaction times were used as parameters of inhibition. Not any of the constituent but symbiotic relation in-between reached the highest inhibition of 90 percent. The pH values of the extracts were 5.1 to 5.4. As a result, the extraction of Calendula officinalis at the optimum conditions can be used effectively in the production of wet wipes and hand sterilizing liquid.

  4. Atividade antiinflamatória do granulado de Calendula officinalis L. e Matricaria recutita L.

    Directory of Open Access Journals (Sweden)

    L.R. Sartori

    2003-01-01

    Full Text Available Este trabalho descreve os resultados da atividade antiinflamatória de um granulado composto pelos extratos secos de Calendula officinalis L. e Matricaria recutita L. (GECOMR® - granulado encapsulado de Calendula officinalis e Matricaria recutita. Estas espécies são utilizadas na medicina popular devido às suas atividades antiinflamatória, antiúlcera gástrica e antiespasmódica. A redução do processo edematogênico nos grupos de animais tratados com o GECOMR foi observada para todos agentes inflamatórios testados (Carragenina, dextrana e histamina. Esta associação mostrou-se eficaz sobre os modelos de edema de pata induzidos pelas diferentes substâncias inflamatórias, com as doses de 100 e 250 mg.kg-1. A associação dos extratos hidroalcoólicos destas espécies medicinais não modificou a indicação isolada de cada uma.

  5. PI3K-mediated proliferation of fibroblasts by Calendula officinalis tincture: implication in wound healing.

    Science.gov (United States)

    Dinda, Manikarna; Dasgupta, Uma; Singh, Namrata; Bhattacharyya, Debasish; Karmakar, Parimal

    2015-04-01

    Calendula officinalis, a member of the Asteraceae family, is a flowering plant and has been used for its antibacterial, antifungal, antiviral, antiinflammatory, anticancer and wound healing activity. The mode of action of C. officinalis tincture on wound healing is poorly understood. Here, we investigated the role of C. officinalis tincture (CDOT) on cell viability and wound closure. C. officinalis tincture stimulated both proliferation and migration of fibroblasts in a statistically significant manner in a PI3K-dependent pathway. The increase in phosphorylation of FAK (Tyr 397) and Akt (Ser 473) was detected after treatment of CDOT. Inhibition of the PI3K pathway by wortmannin and LY294002 decreased both cell proliferation and cell migration. HPLC-ESI MS revealed the presence of flavonol glycosides as the major compounds of CDOT. Altogether, our results showed that CDOT potentiated wound healing by stimulating proliferation and migration of fibroblast in a PI3K-dependent pathway, and the identified compounds are likely to be responsible for wound healing activity. PMID:25641010

  6. The effects of extraction method on recovery rutin from Calendula officinalis L. (Asteraceae

    Directory of Open Access Journals (Sweden)

    Frederico Severino Martins

    2014-01-01

    Full Text Available Background: Calendula officinalis L. (Asteraceae is a Mediterranean specie, but in Europe and America it is cultivated for ornamental or medicinal purposes. This species is widely used for presenting activities, antiinflammatory antibacterial and antioxidant. However the therapeutic action is linked to the amount of assets of the extracted raw material. The extraction method of bioactive compounds is an important step in the manufacturing of herbal medicines, because secondary metabolites with therapeutic potential are usually found in small quantities in plant materials. Objective: Due the medical and commercial importance of C. officinalis, this study aimed to evaluate the impact of the extraction method on the quality of herbal extract and optimize the extraction of rutin from C. officinalis. Materials and Methods : The extraction of rutin was performed by ultrasound and shaker and the optimized conditions were determined by response surface methodology. Results: The results of ultrasound extraction assisted (UEA and maceration dynamic (MD showed that rutin yield ranged from 0.218 to 2.28% (w/w when extract by ultrasound and 0.1-1.44% by MD. The optimal extraction condition for rutin (2.48% to UEA or 1.46% to MD from C. officinalis by UEA or MD were a 19-22 min extraction, ethanol: water ratio of 35-40% and 0.05-0.056 mg/mL to raw material: solvent ratio. Conclusion: The UEA is more efficient to extraction rutin.

  7. Effects of Calendula officinalis Hydroalcoholic Extract on Blood sugar, LDL, HDL and Total cholesterol in Streptozotocin-induced Diabetic Adult male wistar Rats

    OpenAIRE

    M. Mahmoodi; A. Azimi; Shahidi, S

    2014-01-01

    Background & aim: It’s very important to decrease the glucose level and lipid’s serum in diabetes patients by using medicinal plants. In the present study, the effect of hydroalcoholic extract of Calendula officinalis on blood glucose, LDL, HDL and total cholesterol in diabetic rats was examined. Methods: In this experimental study, thirty adult male Wistar rats were randomly divided into control, diabetic and diabetic treated with hydroalcoholic extract of Calendula officinalis (100, 150...

  8. Controversial effects of Calendula officinalis L. on Biochemical and Pathological Factors of Nephropathy in Diabetic Wistar Rats

    Directory of Open Access Journals (Sweden)

    Salehi

    2015-06-01

    Full Text Available Background Chronic hyperglycemia leads to microvascular and macrovascular complications such as diabetic nephropathy. Medicinal plants are good sources for finding new therapeutic chemicals to improve diabetes and relieve its symptoms. Objectives The purpose of the present study was to evaluate the effect of the hydroalcoholic extract (300 mg/kg of Calendula officinalis (marigold on blood biochemical profiles and histopathological changes in kidney of streptozotocin-induced diabetic rats. Materials and Methods Twenty male Wistar rats were divided to four groups: Normal control (NC, diabetic control (DC, normal C. officinalis (N+CO 300 mg/kg, and diabetic C. officinalis (D+CO 300 mg/kg. The rats were treated for a period of 13 weeks. Diabetes was induced by Streptozocin injection, intraperitoneally. Level of glucose, urea, creatinine and also total anti-oxidant capacity, malondialdehyde, total oxidant status in serum and histological alterations in the kidney were analyzed. Results Level of serum glucose, urea, creatinine, malondialdehyde and total oxidant status were increased in diabetic rats, whereas, total anti-oxidant capacity was decreased compared to the control animals. Also, histological findings confirmed the absence of integrity in glomerulus and mass infiltration in kidney tissue in diabetic rats compared to the normal controls. Calendula officinalis extract had no effect on blood glucose, but it decreased blood urea nitrogen and creatinine, total oxidant status and malondialdehyde while it increased total anti-oxidant capacity in the diabetic extract-treated group when compared to diabetic rats. Calendula officinalis could not prevent nephropathy changes in the diabetic rats. Conclusions Therefore, our results suggest that although administration of 300 mg/kg of Calendula officinalis extract showed salutary effects on anti-oxidant profile, yet its protective effects on anti-diabetic and regenerative properties on nephropathy were

  9. Growth, development and content of flavonoids in calendula (Calendula officinalis L.

    Directory of Open Access Journals (Sweden)

    Isabela Cristina Gomes Honório

    2016-01-01

    Full Text Available Flavonoids, the pharmaco-active compounds studied in this work, in general, are located in the shoots as natural pigments that protect the plants from damage by oxidizing agents. The experiment was conducted in the greenhouse of the Fitotecnia Department, Federal University of Viçosa, Minas Gerais Staty. The experimental design was completely randomized with five treatments and five replications. The treatments were the time of harvest: zero, three, six, nine, and twelve days after anthesis (DAA. The height of the plant (mm, stem diameter (mm, number of leaves and total flavonoid content (%, w w-1 were determined. We developed growth curves for calendula with growth behavior assessed through time for height, stem diameter and number of leaves. The time of the harvest season positively influenced the total flavonoid contents and the average productivity of flowers, and the highest content of flavonoids was found three DAA. The average productivity of the flowers and the accumulation of flavonoids were heterogeneous among harvest times (0, 3, 6, 9 and 12 days after anthesis. The highest yield of flavonoids was obtained three days after anthesis; therefore, a harvest of the inflorescences is recommended every three days.

  10. Healing acceleration of acetic acid-induced colitis by marigold (Calendula officinalis in male rats

    Directory of Open Access Journals (Sweden)

    Nader Tanideh

    2016-01-01

    Full Text Available Background/Aim: Ulcerative colitis (UC is a type of chronic inflammatory bowel disease with unknown etiology. Several therapeutic strategies such as consumption of medicinal plants have been used for its treatment. The aim of this study was to evaluate healing effects of Calendula officinalis hydroalcoholic extract in experimentally induced UC in rat. Materials and Methods: Ninety-six rats, weighing 200 ± 20 g, were randomly divided into eight equal groups. UC induced by 3% acetic acid and oral doses of C. officinalis extract, 1500 and 3000 mg/kg, and enema (gel 10% and 20% were given. Two groups as positive controls were given asacol (enema and oral mesalamine. Negative control groups were given normal saline and base gel. On days 3 and 7, intestinal histopathology and weight changes, plus oxidative stress indices including malondialdehyde (MDA level and myeloperoxidase (MPO activity were assayed. Results: A significant increase in the body weight of rats was seen in the group given C. officinalis extract 3000 mg/kg orally, oral mesalamine, and 20% intracolonic gel form of marigold extract compared with negative control and base gel groups during the experimental period. Acute inflammation and granular atrophy after UC induction were resolved completely completely by both 20% intracolonic gel and 3000 mg/kg orally. An increase in MPO activity and a decrease in MDA level in response to oral and intracolonic gel form of C. officinalis were observed 3 and and 7 days after treatment (P < 0.05. Conclusion: Our results indicate that oral and enema forms of hydroalcoholic extract of C. officinalis can be offered as are potential therapeutic agents for UC induced in rats.

  11. Evaluation of Calendula officinalis as an anti-plaque and anti-gingivitis agent

    Directory of Open Access Journals (Sweden)

    Mayur Sudhakar Khairnar

    2013-01-01

    Full Text Available Background: Calendula officinalis (C. officinalis, commonly known as pot marigold, is a medicinal herb with excellent antimicrobial, wound healing, and anti-inflammatory activity. Aim: To evaluate the efficacy of C. officinalis in reducing dental plaque and gingival inflammation. Materials and Methods: Two hundred and forty patients within the age group of 20-40 years were enrolled in this study with their informed consent. Patients having gingivitis (probing depth (PD ≤3 mm, with a complaint of bleeding gums were included in this study. Patients with periodontitis PD ≥ 4 mm, desquamative gingivitis, acute necrotizing ulcerative gingivitis (ANUG, smokers under antibiotic coverage, and any other history of systemic diseases or conditions, including pregnancy, were excluded from the study. The subjects were randomly assigned into two groups - test group (n = 120 and control group (n = 120. All the test group patients were advised to dilute 2 ml of tincture of calendula with 6 ml of distilled water and rinse their mouths once in the morning and once in the evening for six months. Similarly, the control group patients were advised to use 8 ml distilled water (placebo as control mouthwash and rinse mouth twice daily for six months. Clinical parameters like the plaque index (PI, gingival index (GI, sulcus bleeding index (SBI, and oral hygiene index-simplified (OHI-S were recorded at baseline (first visit, third month (second visit, and sixth month (third visit by the same operator, to rule out variable results. During the second visit, after recording the clinical parameters, each patient was subjected to undergo a thorough scaling procedure. Patients were instructed to carry out regular routine oral hygiene maintenance without any reinforcement in it. Results: In the absence of scaling (that is, between the first and second visit, the test group showed a statistically significant reduction in the scores of PI, GI, SBI (except OHI-S (P < 0

  12. Antifungal activity of the essential oil from Calendula officinalis L. (asteraceae growing in Brazil Atividade antifúngica do óleo essencial da Calendula officinalis cultivada no Brasil

    Directory of Open Access Journals (Sweden)

    Zilda Cristiane Gazim

    2008-03-01

    Full Text Available This study tested in vitro activity of the essential oil from flowers of Calendula officinalis using disk-diffusion techniques. The antifungal assay results showed for the first time that the essential oil has good potential antifungal activity: it was effective against all 23 clinical fungi strains tested.Neste estudo foi avaliada a atividade antifúngica do óleo essencial, obtido das flores de Calendula officinalis utilizando-se técnica de difusão em discos. Os resultados dos ensaios antifúngicos mostraram pela primeira vez, que o óleo essencial tem um grande potencial antifúngico, pois foi efetivo contra todas as 23 amostras clínicas de fungos testados.

  13. Subacute effect of cigarette smoke exposure in rats: protection by pot marigold (Calendula officinalis L.) extract.

    Science.gov (United States)

    Ozkol, Halil; Tülüce, Yasin; Koyuncu, Ismail

    2012-02-01

    This study was carried out to determine the preventive effect of Calendula officinalis L. (pot marigold) on rats exposed to cigarette smoke (CS). Rats were divided into three groups as control, CS and CS + pot marigold (PM). The rats in the CS and CS + PM groups were subjected to CS for 1 h twice a day for 23 days. PM (100 mg/kg body weight) was given to rats in the CS + PM group by gavage, 1 h before each administration period. While malondialdehyde, protein carbonyl contents and reduced glutathione level of the CS group increased, their levels diminished by PM administration. In addition, glutathione peroxidase (GPx), superoxide dismutase activities and β-carotene, vitamins A and C levels decreased in the CS group compared to control, however activities of these enzymes and concentration of vitamins were elevated by PM supplementation. This investigation showed that administration of PM supplied relative protection against subacute CS-induced cell injury. PMID:21505008

  14. Componential Profile and Amylase Inhibiting Activity of Phenolic Compounds from Calendula officinalis L. Leaves

    Directory of Open Access Journals (Sweden)

    Daniil N. Olennikov

    2014-01-01

    Full Text Available An ethanolic extract and its ethyl acetate-soluble fraction from leaves of Calendula officinalis L. (Asteraceae were found to show an inhibitory effect on amylase. From the crude extract fractions, one new phenolic acid glucoside, 6′-O-vanilloyl-β-D-glucopyranose, was isolated, together with twenty-four known compounds including five phenolic acid glucosides, five phenylpropanoids, five coumarins, and nine flavonoids. Their structures were elucidated based on chemical and spectral data. The main components, isoquercitrin, isorhamnetin-3-O-β-D-glucopyranoside, 3,5-di-O-caffeoylquinic acid, and quercetin-3-O-(6′′-acetyl-β-D-glucopyranoside, exhibited potent inhibitory effects on amylase.

  15. Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis Utilização de técnicas de cultura de tecidos na proliferação de brotos e enraizamento de Calendula officinalis

    OpenAIRE

    Cristiane Pimentel Victório; Celso Luiz Salgueiro Lage; Alice Sato

    2012-01-01

    The high demand for plant material from Calendula officinalis in the production of herbal medicines and cosmetics, turns the technique of plant-tissue culture into one of the alternatives for the improvement of crops over a short period of time. A protocol for tissue culture was developed from segments of seedlings of C. officinalis, in order to improve the proliferation of shoots and roots. We used a Murashige and Skoog (MS½N) medium, reduced to half the concentrations of NH4NO3 and KNO3 to ...

  16. Fatty acid composition of lipids in pot marigold (Calendula officinalis L. seed genotypes

    Directory of Open Access Journals (Sweden)

    Dulf Francisc V

    2013-01-01

    Full Text Available Abstract Background Calendula officinalis L. (pot marigold is an annual aromatic herb with yellow or golden-orange flowers, native to the Mediterranean climate areas. Their seeds contain significant amounts of oil (around 20%, of which about 60% is calendic acid. For these reasons, in Europe concentrated research efforts have been directed towards the development of pot marigold as an oilseed crop for industrial purposes. Results The oil content and fatty acid composition of major lipid fractions in seeds from eleven genotypes of pot marigold (Calendula officinalis L. were determined. The lipid content of seeds varied between 13.6 and 21.7 g oil/100 g seeds. The calendic and linoleic acids were the two dominant fatty acids in total lipid (51.4 to 57.6% and 28.5 to 31.9% and triacylglycerol (45.7 to 54.7% and 22.6 to 29.2% fractions. Polar lipids were also characterised by higher unsaturation ratios (with the PUFAs content between 60.4 and 66.4%, while saturates (consisted mainly of palmitic and very long-chain saturated fatty acids were found in higher amounts in sterol esters (ranging between 49.3 and 55.7% of total fatty acids. Conclusions All the pot marigold seed oils investigated contain high levels of calendic acid (more than 50% of total fatty acids, making them favorable for industrial use. The compositional differences between the genotypes should be considered when breeding and exploiting the pot marigold seeds for nutraceutical and pharmacological purposes.

  17. Potential of extracts from Saponaria officinalis and Calendula officinalis to modulate in vitro rumen fermentation with respect to their content in saponins.

    Science.gov (United States)

    Budan, Alexandre; Bellenot, Denis; Freuze, Ingrid; Gillmann, Louisa; Chicoteau, Pierre; Richomme, Pascal; Guilet, David

    2014-01-01

    Saponins have the potential to favorably modulate rumen fermentation, but there is generally a lack of the chemical structures associated with the described effects. The activity of extracts from Calendula officinalis and Saponaria officinalis in the rumen was evaluated in vitro. The S. officinalis root extract, reduced CH₄ production by 8.5% and increased total VFA concentration by 25.2%. C. officinalis and S. officinalis root extracts and the S. officinalis aerial part extract decreased the acetate to propionate ratio from 8.6 to 17.4%, according to the extract. An HPLC-ELSD analysis indicated that the saponin content ranged from 43.6 to 57.6 mg/g of dry matter (DM) in the C. officinalis extracts and from 224.0 to 693.8 mg/g of DM in the S. officinalis extracts, expressed as the hederacoside C equivalent. Identification of the saponin compounds present in the extracts by HPLC-MS(n) suggested that the saponin profile modulated the biological activities, showing the importance of determining the structure of saponins when evaluating extracts. PMID:25036683

  18. Anti-inflammatory activity of flower extract of Calendula officinalis Linn. and its possible mechanism of action.

    Science.gov (United States)

    Preethi, Korengath Chandran; Kuttan, Girija; Kuttan, Ramadasan

    2009-02-01

    Calendula officinalis flower extract possessed significant anti-inflammatory activity against carrageenan and dextran-induced acute paw edema. Oral administration of 250 and 500 mg/kg body weight Calendula extract produced significant inhibition (50.6 and 65.9% respectively) in paw edema of animals induced by carrageenan and 41.9 and 42.4% respectively with inflammation produced by dextran. In chronic anti-inflammatory model using formalin, administration of 250 and 500 mg/kg body weight Calendula extract produced an inhibition of 32.9 and 62.3% respectively compared to controls. TNF-alpha production by macrophage culture treated with lipopolysaccharide (LPS) was found to be significantly inhibited by Calendula extract. Moreover, increased levels of proinflammatory cytokines IL- 1beta, IL-6, TNF-alpha and IFN-gamma and acute phase protein, C- reactive protein (CRP) in mice produced by LPS injection were inhibited significantly by the extract. LPS induced cyclooxygenase-2 (Cox-2) levels in mice spleen were also found to be inhibited by extract treatment. The results showed that potent anti-inflammatory response of C. officinalis extract may be mediated by the inhibition of proinflammatory cytokines and Cox-2 and subsequent prostaglandin synthesis. PMID:19374166

  19. Análise de amostras de flores de Calêndula (Calendula officinalis L., Asteraceae) comercializadas na grande Curitiba

    OpenAIRE

    Lais Mondadori Otramario Santos; Luciana Aparecida de Oliveira; Eliane Patrícia Sichinel Tibulo; Cristina Peitz de Lima

    2015-01-01

    A espécie Calendula officinalis L. é uma planta herbácea pertencente à família Asteraceae, suas flores apresentam propriedades antimicrobianas, emolientes, antiinflamatórias e tonificantes da pele. Os flavonoides são utilizados como marcadores para avaliar a qualidade da droga vegetal. O objetivo do presente trabalho foi avaliar a identidade e qualidade de seis amostras de flores de C. officinalis L. comercializadas na Grande Curitiba (PR). Foram realizados os ensaios de identificação macrosc...

  20. The Effect of Calendula Officinalis in Therapy of Acetic Acid Induced Ulcerative Colitis in Dog as an Animal Model

    OpenAIRE

    Mehrabani, D; M. Ziaei; Hosseini, S.V; Ghahramani, L; Bananzadeh, A M; Ashraf, M. J.; Amini, A; Amini, M; Tanideh, N

    2011-01-01

    Background In patients with ulcerative colitis (UC), the repeated cycle of injury and repair of intestinal mucosa has been reported to increase the risk of colon cancer. So, a safe and efficient therapy is required for the treatment and prophylaxis for the disease.This study aims to investigate the efficacy of Calendula officinalis extract in treatment of experimentally induced ulcerative colitis in dog animal model. Methods During fall 2010, 10 out-bred female German dogs (1-2 years old; wei...

  1. Wound Healing and Anti-Inflammatory Effect in Animal Models of Calendula officinalis L. Growing in Brazil

    OpenAIRE

    Leila Maria Leal Parente; Ruy de Souza Lino Júnior; Leonice Manrique Faustino Tresvenzol; Marina Clare Vinaud; José Realino de Paula; Neusa Margarida Paulo

    2012-01-01

    Calendula officinalis is an annual herb from Mediterranean origin which is popularly used in wound healing and as an anti-inflammatory agent. In this study, the ethanolic extract, the dichloromethane, and hexanic fractions of the flowers from plants growing in Brazil were produced. The angiogenic activity of the extract and fractions was evaluated through the chorioallantoic membrane and cutaneous wounds in rat models. The healing activity of the extract was evaluated by the same cutaneous wo...

  2. Usage of Calendula officinalis in the prevention and treatment of radiodermatitis: a randomized double-blind controlled clinical trial

    OpenAIRE

    Franciane Schneider; Mitzy Tannia Reichembach Danski; Stela Adami Vayego

    2015-01-01

    OBJECTIVE To evaluate the efficacy of Calendula officinalis in relation to Essential Fatty Acids for the prevention and treatment of radiodermatitis. METHOD This is a randomized double-blind controlled clinical trial with 51 patients with head and neck cancer in radiotherapy treatment divided into two groups: control (27) and experimental (24). RESULTS There is statistically significant evidence (p-value = 0.0120) that the proportion of radiodermatitis grade 2 in Essential Fatty Acids group i...

  3. Bioefficacy of Calendula officinalis Linn. (Asteraceae) extracts in the control of Spodoptera litura Fabricus (Noctuidae: Lepidoptera) under laboratory conditions.

    OpenAIRE

    Doddamane Manjulakumari; Medhini N; Divakara Y.G.; Prabha D

    2012-01-01

    The effect of Calendula officinalis L on the nutritional physiological parameters of Spodoptera litura Fab larvae were studied by treating with leaf and flower extracts in various solvents. Protease and amylase enzyme activities along with their respective substrates were assayed in hemolymph and midgut of treated and untreated larvae. Irrespective of tissues, all the extracts showed significant reduction in the biochemical profile compared to controls. The most significant change was observe...

  4. Evaluation of In Vitro Anticancer Activity of Ocimum Basilicum, Alhagi Maurorum, Calendula Officinalis and Their Parasite Cuscuta Campestris

    OpenAIRE

    Mandana Behbahani

    2014-01-01

    The present investigation was carried out to study the relationship between presence of cytotoxic compounds in Ocimum basilicum, Alhagi maurorum, Calendula officinalis and their parasite Cuscuta campestris. The cytotoxic activity of the pure compounds was performed by MTT assay against breast cancer cell lines (MCF-7 and MDA-MB-231) and normal breast cell line (MCF 10A). The induction of apoptosis was measured by the expression levels of p53, bcl-2, bax and caspase-3 genes using quantitative ...

  5. Effect of Calendula officinalis hydroalcoholic extract on passive avoidance learning and memory in streptozotocin-induced diabetic rats

    OpenAIRE

    Shirin Moradkhani; Iraj Salehi; Somayeh Abdolmaleki; Alireza Komaki

    2015-01-01

    Background: Medicinal plants, owing to their different mechanisms such as antioxidants effects, may improve learning and memory impairments in diabetic rats. Calendula officinalis (CO), has a significant antioxidant activity. Aims: To examine the effect of hydroalcoholic extract of CO on passive avoidance learning (PAL) and memory in streptozotocin (STZ)-induced diabetic male rats. Settings and Design: A total of 32 adult male Wistar rats were randomly allocated to four groups: Contro...

  6. INVESTIGATION OF DIURETIC ACTIVITY OF PHYTOPREPARATIONS OF CALENDULA OFFICINALIS L. FLOWERS

    Directory of Open Access Journals (Sweden)

    V. A. Kurkin

    2016-01-01

    Full Text Available The flowers of the pot marigold (Calendula officinalis L. are part of combination herbal medicinal preparations which applied in the treatment of diseases of the hepatobiliary system, gastrointestinal tract, diseases of kidneys and the urinary tract, such as «Fitonephrol» («Urological tea». Calendula flowers make a contribution in the total therapeutic activity of «Urological tea» as a component which has anti-inflammatory and reparative properties. As is widely known that phytopharmaceuticals usually combine the variety of therapeutic activity and softness of pharmacological effects along with the absence of a significant number of side effects and contraindications which is extremely important in the treatment of chronic pathologies. The main purpose was to study diuretic activity of phytopreparations of pot marigold.Materials and methods. Investigations of discovering of diuretic action of pot marigold’s phytopreparations were carried out on white rats of both sexes weighing 200–220 g (8 sets of experiments. Each experimental group consisted of ten animals. The effect of pot marigold medicines at a dose of 50 mg/kg was carried out background the administered intragastrically at 3% aqueous load of 4 and 24 hours of the experiment. There are such drugs as a subjects to examination: calendula tincture (1 : 10 (industrial production, calendula tincture (1 : 5 and calendula liquid extract (1 : 2 of 70% alcohol, obtained at the Department of Pharmacognosy with Botany and Bases of Phytotherapy, at a dose of 50 mg/kg. Animals were housed in the vivarium on a standard diet with free access to water. The day before the experiment the rats received intragastric water load in the amount of 3% of body weight. On the day of the experiment the animals of the control group was administered water-alcohol load and experienced – intragastrically drug dose of 50 mg/kg in an identical volume of water. As a control was used 70% alcohol. Animals were

  7. Study regarding the effect of calendula officinalis cream in healing of pressure sores

    Directory of Open Access Journals (Sweden)

    Ravanbakhsh Esmaili

    2008-01-01

    Full Text Available (Received 13 July, 2008 ; Accepted 29 Oct, 2008 Abstract Background and Purpose: Pressure sore is common among patients with prolonged stay in hospitals or homes. Treatment of such sores is costly and performed by various procedures. Considering the effective role of calendula officinalis in treatment of sores, ulcers and cutaneous inflammations, this study is conducted to determine the effect of this cream in healing pressure sores.Materials and methods: This is a pre/post comparative clinical trial done on 20 patients with pressure sores who received the recommended treatment.The condition of patients such as, duration of pressure sore, and the extent of the sore were recorded. The sore was washed with normal saline, dried, followed by applying calendula cream three times a day for a duration of 4 weeks. Each week, the extent and rate of healing was observed and recorded.Results: The rate of healing in patients under our study was 56.6%. Majority (55% of patients had partial healing. The time of healing was in weeks 3.5±1.2 (P<0.001. The complete healing occurred in the 3rd and 4th week, subsequent to the beginning of the treatment.Conclusion: Considering the availability of the cream and its affordability, it can be used in the treatment of pressure sores. In addition, it can be used as a drug for treatment of patients who are in hospitals and/or in their homes. J Mazand Univ Med Sci 2008; 18(66:19-25(Persian

  8. Antioxidant capacity of calendula officinalis flowers extract and prevention of radiation induced oropharyngeal mucositis in patients with head and neck cancers: a randomized controlled clinical study

    OpenAIRE

    Mohammad Taghi Kazemi; Mahmoud Baradaran; Ali Bijani; Yasaman Moghadamnia; Fatemeh Vejdani; Mohammad Khalilpour; Dariush Moslemi; Neda Babaee; Asieh Khalilpour; Mahdi Pouramir; Ali Akbar Moghadamnia

    2013-01-01

    This study was designed to determine the effect of Calendula officinalis flowers extract mouthwash as oral gel on radiation-induced oropharyngeal mucositis (OM) in patients with head-and-neck cancer. Forty patients with neck and head cancers under radiotherapy or concurrent chemoradiotherapy protocols were randomly assigned to receive either 2% calendula extract mouthwash or placebo (20 patients in each group). Patients were treated with telecobalt radiotherapy at conventional fractionation (...

  9. Chemical characterization and bioactive properties of two aromatic plants: Calendula officinalis L. (flowers) and Mentha cervina L. (leaves).

    Science.gov (United States)

    Miguel, María; Barros, Lillian; Pereira, Carla; Calhelha, Ricardo C; Garcia, Pablo A; Castro, MaÁngeles; Santos-Buelga, Celestino; Ferreira, Isabel C F R

    2016-05-18

    The chemical composition and bioactive properties of two plants (Calendula officinalis L. and Mentha cervina L.) were studied. Their nutritional value revealed a high content of carbohydrates and low fat levels, and very similar energy values. However, they presented different profiles in phenolic compounds and fatty acids; C. officinalis presented mainly glycosylated flavonols and saturated fatty acids, while M. cervina presented mainly caffeoyl derivatives and polyunsaturated fatty acids. M. cervina showed the highest concentration of phenolic compounds while C. officinalis presented higher amounts of sugars, organic acids and tocopherols. The highest antioxidant and cytotoxic activities were obtained for the hydromethanolic extract of M. cervina, which presented the lowest values of EC50 and exhibited cytotoxicity against the four tumor cell lines tested. Infusions showed no cytotoxicity for the tumor cell lines, and none of the extracts showed toxicity against non-tumor cells. This study contributes to expand the knowledge on both natural sources and therefore their use. PMID:27110832

  10. Extracts from Calendula officinalis offer in vitro protection against H2 O2 induced oxidative stress cell killing of human skin cells.

    Science.gov (United States)

    Alnuqaydan, Abdullah M; Lenehan, Claire E; Hughes, Rachel R; Sanderson, Barbara J

    2015-01-01

    The in vitro safety and antioxidant potential of Calendula officinalis flower head extracts was investigated. The effect of different concentrations (0.125, 0.5, 1.0, 2.0 and 5.0% (v/v)) of Calendula extracts on human skin cells HaCaT in vitro was explored. Doses of 1.0% (v/v) (0.88 mg dry weight/mL) or less showed no toxicity. Cells were also exposed to the Calendula extracts for either 4, 24 or 48 h before being exposed to an oxidative insult (hydrogen peroxide H2 O2 ) for 1 h. Using the MTT cytotoxicity assay, it was observed that two independent extracts of C. officinalis gave time-dependent and concentration-dependent H2 O2 protection against induced oxidative stress in vitro using human skin cells. Pre-incubation with the Calendula extracts for 24 and 48 h increased survival relative to the population without extract by 20% and 40% respectively following oxidative challenge. The antioxidant potential of the Calendula extracts was confirmed using a complimentary chemical technique, the DPPH(●) assay. Calendula extracts exhibited free radical scavenging abilities. This study demonstrates that Calendula flower extracts contain bioactive and free radical scavenging compounds that significantly protect against oxidative stress in a human skin cell culture model. PMID:25266574

  11. Extracts from Calendula officinalis offer in vitro protection against H2 O2 induced oxidative stress cell killing of human skin cells.

    Science.gov (United States)

    Alnuqaydan, Abdullah M; Lenehan, Claire E; Hughes, Rachel R; Sanderson, Barbara J

    2015-01-01

    The in vitro safety and antioxidant potential of Calendula officinalis flower head extracts was investigated. The effect of different concentrations (0.125, 0.5, 1.0, 2.0 and 5.0% (v/v)) of Calendula extracts on human skin cells HaCaT in vitro was explored. Doses of 1.0% (v/v) (0.88 mg dry weight/mL) or less showed no toxicity. Cells were also exposed to the Calendula extracts for either 4, 24 or 48 h before being exposed to an oxidative insult (hydrogen peroxide H2 O2 ) for 1 h. Using the MTT cytotoxicity assay, it was observed that two independent extracts of C. officinalis gave time-dependent and concentration-dependent H2 O2 protection against induced oxidative stress in vitro using human skin cells. Pre-incubation with the Calendula extracts for 24 and 48 h increased survival relative to the population without extract by 20% and 40% respectively following oxidative challenge. The antioxidant potential of the Calendula extracts was confirmed using a complimentary chemical technique, the DPPH(●) assay. Calendula extracts exhibited free radical scavenging abilities. This study demonstrates that Calendula flower extracts contain bioactive and free radical scavenging compounds that significantly protect against oxidative stress in a human skin cell culture model.

  12. The performance of young pigs fed different amounts of marigold (Calendula officinalis) meal; a pilot study

    OpenAIRE

    Hindle, V.A.; Mathijssen-Kamman, A.A.; STOCKHOFE, N.; Cone, J.W.

    2002-01-01

    Hexane-extracted calendula meal was tested in an acceptance trial with pigs to determine their response to calendula meal. Performance parameters included feed intake, daily growth and post mortem histopathological examination of vital organs. Although calendula meal showed potential as a ration ingredient for young pigs it is advised not to include more than 10% of it in the diet

  13. Biocontrol and Plant Growth Promotion Characterization of Bacillus Species Isolated from Calendula officinalis Rhizosphere.

    Science.gov (United States)

    Ait Kaki, Asma; Kacem Chaouche, Noreddine; Dehimat, Laid; Milet, Asma; Youcef-Ali, Mounia; Ongena, Marc; Thonart, Philippe

    2013-12-01

    The phenotypic and genotypic diversity of the plant growth promoting Bacillus genus have been widely investigated in the rhizosphere of various agricultural crops. However, to our knowledge this is the first report on the Bacillus species isolated from the rhizosphere of Calendula officinalis. 15 % of the isolated bacteria were screened for their important antifungal activity against Fusarium oxysporum, Botrytis cinerea, Aspergillus niger, Cladosporium cucumerinium and Alternaria alternata. The bacteria identification based on 16S r-RNA and gyrase-A genes analysis, revealed strains closely related to Bacillus amyloliquefaciens, B. velezensis, B. subtilis sub sp spizezenii and Paenibacillus polymyxa species. The electro-spray mass spectrometry coupled to liquid chromatography (ESI-LC MS) analysis showed that most of the Bacillus isolates produced the three lipopeptides families. However, the P. polymyxa (18SRTS) didn't produce any type of lipopeptides. All the tested Bacillus isolates produced cellulase but the protease activity was observed only in the B. amyloliquefaciens species (9SRTS). The Salkowsky colorimetric test showed that the screened bacteria synthesized 6-52 μg/ml of indole 3 acetic acid. These bacteria produced siderophores with more than 10 mm wide orange zones on chromazurol S. The greenhouse experiment using a naturally infested soil with Sclerotonia sclerotiorum showed that the B. amyloliquefaciens (9SRTS) had no significant (P > 0.05) effect on the pre-germination of the chickpea seeds. However, it increased the size of the chickpea plants and reduced the stem rot disease (P officinalis and other crop systems. PMID:24426149

  14. Bioefficacy of Calendula officinalis Linn. (Asteraceae extracts in the control of Spodoptera litura Fabricus (Noctuidae: Lepidoptera under laboratory conditions.

    Directory of Open Access Journals (Sweden)

    Doddamane Manjulakumari

    2012-08-01

    Full Text Available The effect of Calendula officinalis L on the nutritional physiological parameters of Spodoptera litura Fab larvae were studied by treating with leaf and flower extracts in various solvents. Protease and amylase enzyme activities along with their respective substrates were assayed in hemolymph and midgut of treated and untreated larvae. Irrespective of tissues, all the extracts showed significant reduction in the biochemical profile compared to controls. The most significant change was observed in chloroform and petroleum ether leaf and flower extracts treated larvae compared to other extracts suggesting their components affect nutritional physiology of S.litura. Hence, Calendula extracts can not only be used to treat human ailments but also as an effective botanical pesticide.

  15. Production of oleanolic acid glycosides by hairy root established cultures of Calendula officinalis L.

    Science.gov (United States)

    Długosz, Marek; Wiktorowska, Ewa; Wiśniewska, Anita; Pączkowski, Cezary

    2013-01-01

    In order to initiate hairy root culture initiation cotyledons and hypocotyls of Calendula officinalis L. were infected with Agrobacterium rhizogenes strain ATCC 15834 or the same strain containing pCAMBIA 1381Z vector with β-glucuronidase reporter gene under control of promoter of NIK (Nematode Induced Kinase) gene. The efficiency of induction of hairy roots reached 33.8% for cotyledons and 66.6% for hypocotyls together for both transformation experiments. Finally, eight control and nine modified lines were established as a long-term culture. The hairy root cultures showed the ability to synthesize oleanolic acid mainly (97%) as glycosides; control lines contained it at the average 8.42 mg · g(-1) dry weight in tissue and 0.23 mg · dm(-3) in medium; modified lines: 4.59 mg · g(-1) for the tissue, and 0.48 mg · dm(-3) for the medium. Additionally lines showed high positive correlation between dry/fresh weight and oleanolic acid concentration in tissue. Using the Killiani mixture in acidic hydrolysis of oleanolic acid glycosides released free aglycones that were partially acetylated in such conditions. PMID:24040627

  16. Magnetic molecularly imprinted polymer for the selective extraction of quercetagetin from Calendula officinalis extract.

    Science.gov (United States)

    Ma, Run-Tian; Shi, Yan-Ping

    2015-03-01

    A new magnetic molecularly imprinted polymers (MMIPs) for quercetagetin was prepared by surface molecular imprinting method using super paramagnetic core-shell nanoparticle as the supporter. Acrylamide as the functional monomer, ethyleneglycol dimethacrylate as the crosslinker and acetonitrile as the porogen were applied in the preparation process. Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD) and Vibrating sample magnetometer (VSM) were applied to characterize the MMIPs, and High performance liquid chromatography (HPLC) was utilized to analyze the target analytes. The selectivity of quercetagetin MMIPs was evaluated according to their recognition to template and its analogues. Excellent binding for quercetagetin was observed in MMIPs adsorption experiment, and the adsorption isotherm models analysis showed that the homogeneous binding sites were distributed on the surface of the MMIPs. The MMIPs were employed as adsorbents in solid phase extraction for the determination of quercetagetin in Calendula officinalis extracts. Furthermore, this method is fast, simple and could fulfill the determination and extraction of quercetagetin from herbal extract. PMID:25618718

  17. Antioxidant polyphenol and flavone contents in correlation with cultivation technology for Calendula officinalis L.

    Directory of Open Access Journals (Sweden)

    Ersilia ALEXA

    2009-05-01

    Full Text Available During the last two decades there has been a strong trend to returning to plant therapy. Research highlight polyphenol and flavone content of Calendula officinalis L playing an important role in protecting man’s health. Natural antioxidants are spread particularly in the plant kingdom[2]. Bio-flavones and polyphenols are man’s main allies in his fight against all threats to body’s health, including viruses, cancer,, toxic substances and micro-organisms. Antioxidant action of polyphenols is largely used in medicine and pharmacy as deactivating agents of ultraviolet radiation and metal ions. These substances decompose peroxides and inhibit the forming action of free radicals, thus hindering disease appearance [3]. The paper aims to establish that the yield capacity of the analysed cultivars was significant influenced by the cultivation technology. Sowing densities is one of the most important technological step for marigold. Fertilisation also had great influence on yield capacity. Concerning the polyphenol content and the flavones content we can observe that the technology had great impact on their quantity and quality, so in order to obtain best results we should take in consideration the fact that is vital to improve the cultivation technology for marigold. The quality of marigold yield is given by the content of active elements from the inflorescences. From these elements we study in particularly the polyphenols and the flavones.

  18. The Effects of Age on the Yield and Composition of the Essential Oils of Calendula officinalis

    Science.gov (United States)

    Okoh, O. O.; Sadimenko, A. A.; Afolayan, A. J.

    The variations in the yield and composition of the essential oils from Calendula officinalis L. (Asteraceae) cultivated in Alice, Eastern Cape of South Africa are reported. Essential oils of this plant were obtained by hydrodistillation using the Clevenger apparatus and analysis was performed by GC-MS. The yield in essential oil showed a maximum at the full flowering stage (0.97%) and a minimum during the pre-flowering stage (0.13%). The compositions also showed different patterns at different phases of the vegetative cycle. Sesquiterpenes (α-cadinene, α-cadinol, T-muurolol and epi-bicyclosesquiphellandrene) and monoterpenes (limonene, 1, 8-cineole and trans-β-ocimene) showed the highest correlations with the age of the plant. Aiming the use of essential oil as a food ingredient, the most interesting stage is the post-flowering period, the essential oil at this time being rich in α-cadinene, α-cadinol, t-muurolol, limonene, 1,8-cineole, with P-cymene present at lower levels. α-cadinene is an important flavouring agent in baked foods, candy and chewing gum and also used as a fragrance in cosmetics and detergents. T-muurolol and α-cadinol are important antimicrobial agents.

  19. PARAMETERS OF THE IMMUNOLOGICAL PROFILE IN CHICKENS TREATED WITH A CALENDULA OFFICINALIS EXTRACTION

    Directory of Open Access Journals (Sweden)

    C DUMITRU

    2002-11-01

    Full Text Available Tests were carried out on thirty, 42 days old Rock x Cornish chickens, divided into three experimental groups: I - control injected with saline, II - injected with a Calendula officinalis extraction supplemented with Bayol and III - treated with Bayol. The effects of the in vivo treatments and simultaneous antigen priming (0.5 ml of a 5 % suspension of SRBC on their humoral (lysozyme, anti-SRBC antibodies and cell-mediated (leucocyte blast transformation responses were investigated. Lysozyme (51.50 ± 32.30, 56.80 ± 41.27, 29.50 ± 22.73 μg/ml(p<0.05, and anti-SRBC antibody titers (2.35 ± 0.86, 2.05 ± 0.65 and 1.90 ± 0.55 were the lowest in Bayol treated group. Spontaneous stimulation index was positively influenced by the in vivo Bayol therapy (57.20 ± 8.88 per cent, when compared to that recorded for the other groups (31.06 ± 18.93 group I, 33.37 ± 19.26 group II.

  20. Light Absorption and Carotenoid Synthesis of Pot Marigold (Calendula officinalis L.) in Response to Phosphorous and Potassium Varying Levels

    OpenAIRE

    Mohammad SEDGHI; Alireza PIRZAD; Bahman AMANPOUR-BALANEJI

    2011-01-01

    In order to provide additional information on the effects of elemental deficiency on factors that affect plant production in medicinal plants, a factorial field experiment as randomized complete block design was conducted on Calendula officinalis. Treatments were four phosphorus levels (P2O5) including 0, 40, 80 and 120 Kg ha-1 and four potassium levels (K2O) as 0, 50, 100 and 150 Kg ha-1. Results showed that applied treatments had significant effects on petal carotenoids and the highest amou...

  1. Effect of Calendula officinalis Flower Extract on Acute Phase Proteins, Antioxidant Defense Mechanism and Granuloma Formation During Thermal Burns

    OpenAIRE

    Chandran, Preethi K.; Kuttan, Ramadasan

    2008-01-01

    Effect of Calendula officinalis flower extract was investigated against experimentally induced thermal burns in rats. Burn injury was made on the shaven back of the rats under anesthesia and the animals were treated orally with different doses of the flower extract (20 mg, 100 mg and 200 mg/kg body weight). The animals treated with the extract showed significant improvement in healing when compared with the control untreated animals. The indicators of the wound healing such as collagen-hydrox...

  2. A Bio-Guided Fractionation to Assess the Inhibitory Activity of Calendula officinalis L. on the NF-κB Driven Transcription in Human Gastric Epithelial Cells

    OpenAIRE

    Elisa Colombo; Enrico Sangiovanni; Michele D’Ambrosio; Enrica Bosisio; Alexandru Ciocarlan; Marco Fumagalli; Antonio Guerriero; Petru Harghel; Mario Dell’Agli

    2015-01-01

    Calendula officinalis L. has been largely known for its topical anti-inflammatory properties; however, there are no experimental evidences about its antiphlogistic effect at the gastric level. To investigate whether marigold might exert an activity against gastric inflammation, a CH2Cl2 extract obtained from C. officinalis flowers was evaluated in vitro on the NF-κB pathway. The lipophilic extract demonstrated a significant inhibitory effect on the NF-κB driven transcription. The identificati...

  3. Physiological response of marigold (calendula officinalis L.) plants to gamma radiation, gibberellic acid and kinetin

    International Nuclear Information System (INIS)

    This study was carried out during the two successive seasons of 2005/2006 and 2006/2007 at the Experimental Field of Plant Research Department, Nuclear Research Center, Atomic Energy Authority at Inshas in a newly reclaimed sandy loam soil. The aim of this work was to study the effect of gamma radiation, gibberellic acid or kinetin and their interaction on the growth, flowering and the productivity of pot-marigold (Calendula officinalis L.) plants. The experimental trials included two factorial experiments; the first one was to study the effect of gamma radiation and gibberellic acid and the interaction between them on pot-marigold plants. Another experiment was conducted to study the effect of gamma radiation and kinetin and the interaction between them on pot-marigold plants. Pot-marigold seeds were irradiated before sowing with gamma rays at 0, 50, 100, 150 or 200 Gray (Gy) of gamma rays. After planting, plants were sprayed with either gibberellic acid (at the concentrations of 0, 50, 100, 150 or 200 ppm) or kinetin (at the concentrations of 0, 10, 20, 30 or 40 ppm). Generally, gamma rays treatments (50, 100 and 150 Gy) increased plant height, branch number/plant, leaf area, vegetative growth and roots fresh and dry weights of pot-marigold plants. Also, the same gamma doses accelerated flowering and decreased the period from sowing until flowering while increased flower head diameter, flower number/plant and flowers fresh and dry weights per plant and per feddan. In addition, gamma rays (50 - 150 Gy) increased volatile oil yield in flowers, leaf chlorophyll content, carotenoids and beta carotene in flowers, total soluble sugars and NPK %. The best values were obtained by 50 Gy dose of gamma rays, whereas the dose of 200 Gy gave the lowest values.

  4. Thin-layer chromatography analysis and scavenging activity of marigold (Calendula officinalis L extracts

    Directory of Open Access Journals (Sweden)

    Ćetković Gordana S.

    2003-01-01

    Full Text Available The methanol, petroleum ether, chloroform, ethyl acetate, n-butanol and water extracts were obtained by extraction of marigold flower (Calendula officinalis L. The content of total phenolic compounds, determined by UV spectrophotometric method using the Folin-Ciocalteu reagent, was 15.12 mg/g. The content of total flavonoids, determined by UV spectrophotometric method according to Markham, was 5.13 mg/g. Qualitative determination of phenolic compounds in the extracts was performed by one- and two-dimensional thin-layer chromatography (TLC procedures. The results of one- and two-dimensional TLC analyses showed that different flavonoids and phenolic acids were present in the investigated extracts. The greatest number of flavonoids (rutin, quercetin and some unidentified flavonoid glycosides and phenolic acids (chlorogenic, caffeic, coumaric and vanillic acid were deteminated in methanol extract. The influence of marigold extracts, in concentration range 0.6-1.2 mg/mL, on 2,2’-diphenyl-1-picrylhydrazyl (DPPH free radicals was investigated by electron spin resonance (ESR spectroscopy. All extracts showed scavenging activity (SA in the following order: ethyl acetate > n-butanol > methanol > water > chloroform > petroleum ether. The SA increased with increasing concentration of extracts. The ethyl acetate and n-butanol extracts exibited the most significant SA. These extracts in concentration of 1.2 mg/mL eliminated completely DPPH radicals. The lowest SA had chloroform and petroleum ether extracts (in concentration of 0.6 mg/mL SA=0%. The SA of marigold extracts is attributed to its hydrogen-donating ability and scavenging effect.

  5. An investigation of CO2 extraction of marigold (Calendula officinalis L.

    Directory of Open Access Journals (Sweden)

    LIDIJA PETROVIC

    2007-04-01

    Full Text Available Essential oil content (0.05 % of marigold (Calendula officinalis L. was determined using the official steam distillation procedure. High-pressure CO2 extraction of plant material under supercritial (100, 200 and 300 bar and 40 °C and subcritical (60, 90 and 120 bar and 15 °C conditions for 3 h was investigated. It was found that the increase in the pressure promoted an increase in the yield. The essential oil contents obtained from the investigated CO2 extracts by steam distillation were significantly higher (1.52–2.70 times and increased with pressure.Major constituents of the oil, identified using GC-MS and GC-FID, were a-cadinol (26.54 %, T-cadinol and T-muurolol (9.80 %, g-cadinene (2.99 %, hexadecanoic acid (2.95 %, and ledane (2.45 %. In addition, the essential oils of the CO2 extracts contained d-cadinene (6.50–19.87 % under supercritical and 16.09–19.41 % under subcritical conditions, which was not found in the essential oil obtained from the plant by steam distillation. The extraction kinetics was investigated at 200 bar and 40 °C. The total extract obtained after 10 h of extraction was 6.54 % and essential oil content in it, refering to plant material, was 0.209 %, which is 4.16 times more than the one determined by the standard steam distillation procedure.

  6. Spectral difference method applied in determination of beta-carotene, chlorophyll a and b from irradiated seeds of Calendula Officinalis

    International Nuclear Information System (INIS)

    Beta-carotene is part of the class of the carotenoid pigments that have a special function in the pigmentation of flowers and fruits. These pigments are essential in photosynthesis where they are retaining energy and are photoprotectors by forming the redox system. They are also precursors of vitamin A, pigments involved in the process of seeing. Beta-carotene is responsible for the growth and development of organisms, for the protection and recovery of epithelial tissue, etc. Next to chlorophyll, carotenoids are the major components of the complex pigment-protein from the thylacoide membrane, and some of them, such as beta-carotene, were for a long time considered to be protecting the membrane against destructive events caused by the over excitement of the chlorophyll. This paper presents spectral difference method for physico-chemical characterisation of three components (beta-carotene, chlorophyll a and b) from Calendula officinalis leaves and stalks, after irradiation of germinated seeds. The germinated seeds of Calendula officinalis has been irradiated at 8 different doses ( 1 k Rad, 3 k Rad, 5 k Rad, 10 k Rad, 20 k Rad, 50 k Rad, 100 k Rad, 500 krad), one set of seeds remaining unirradiated for comparison. The plants had been grown in the same conditions of light, temperature and humidity. The identification of beta-carotene was performed through thin layer chromatography. The chemical concentrations of beta-carotene, chlorophyll a and b were determined by spectral difference method. (authors)

  7. Total antioxidant and oxidant status of plasma and renal tissue of cisplatin-induced nephrotoxic rats: protection by floral extracts of Calendula officinalis Linn.

    Science.gov (United States)

    Verma, Pawan Kumar; Raina, Rajinder; Sultana, Mudasir; Singh, Maninder; Kumar, Pawan

    2016-01-01

    The present study was aimed to determine the total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) of plasma and renal tissue in cisplatin (cDDP) induced nephrotoxic rats and its protection by treatments with floral extracts of Calendula officinalis Linn. Treatment with cDDP elevated (p officinalis along with cDDP restored (p > 0.05) CR, albumin, TOS, GSH and activities of antioxidant enzymes in blood and renal tissue. Ethanolic extract treatments reduced (p officinalis protect cDDP induced nephrotoxicity by restoring antioxidant system of the renal tissue. PMID:26513373

  8. Antimicrobial activity of Calendula officinalis petal extracts against fungi, as well as Gram-negative and Gram-positive clinical pathogens.

    Science.gov (United States)

    Efstratiou, Efstratios; Hussain, Abdullah I; Nigam, Poonam S; Moore, John E; Ayub, Muhammad A; Rao, Juluri R

    2012-08-01

    The aim of the present study was to assess the antimicrobial activity of methanol and ethanol extracts of pot marigold (Calendula officinalis) petals against clinical pathogens. The antimicrobial potential of C. officinalis extracts was evaluated against a panel of microorganisms isolated from patients at the Belfast City Hospital (BCH), including bacteria and fungi, using disc diffusion assay. Methanol extract of C. officinalis exhibited better antibacterial activity against most of the bacteria tested, than ethanol extract. Both methanol and ethanol extracts showed excellent antifungal activity against tested strains of fungi, while comparing with Fluconazole. PMID:22789794

  9. Impact of exogenous salicylic acid on growth and ornamental characteristics of calendula (Calendula officinalis L. under salinity stress

    Directory of Open Access Journals (Sweden)

    Bayat H.

    2012-04-01

    Full Text Available Application of salicylic acid (SA as a phytohormone has been increased due to resistance to stresses such as salt stress. Pot experiments were conducted to determine the effect of exogenous salicylic acid application on growth and ornamental characteristics of calendula grown under salt stress and greenhouse conditions. For this purpose a factorial experiment based on completely randomized design was conducted with 3 levels of SA (0 (control, 1, 2 mM and 3 levels of NaCl (0, 100 and 200 mM with 4 replications. At flowering stage, SA was applied with spraying two times in two week intervals. NaCl was also applied as drench (200 ml per pot in two day intervals. The results showed that salinity decreased the growth, Chlorophyll reading values, flower number per plant and flower diameter. However, foliar applications of SA resulted in greater root, shoot and total dry weight, plant height and leaf area of calendula plants under salt stress. The highest chlorophyll reading values was obtained from 2.00 mM SA application in all NaCl treatments. Salinity decreased number of flower per plant and flower diameter as ornamental characteristics; however SA increased them under salinity stress. Plants treated with 1.00 mM SA had the highest flower diameter at 100 and 200 mM of NaCl. Electrolyte leakage increased by salinity, however foliar application of SA significantly reduced electrolyte leakage under salt stress. Based on the present results, foliar application of SA treatments can ameliorate the negative effects of salinity on the growth and ornamental characteristics of calendula plants.

  10. IMPACT OF EXOGENOUS SALICYLIC ACID ON GROWTH AND ORNAMENTAL CHARACTERISTICS OF CALENDULA (CALENDULA OFFICINALIS L.) UNDER SALINITY STRESS

    OpenAIRE

    Bayat H.; Alirezaie, M.; H. Neamati

    2012-01-01

    Application of salicylic acid (SA) as a phytohormone has been increased due to resistance to stresses such as salt stress. Pot experiments were conducted to determine the effect of exogenous salicylic acid application on growth and ornamental characteristics of calendula grown under salt stress and greenhouse conditions. For this purpose a factorial experiment based on completely randomized design was conducted with 3 levels of SA (0 (control), 1, 2 mM) and 3 levels of NaCl (0, 100 and 200 mM...

  11. Antibacterial activity of antipsoriatic herbs: Cassia tora, Momordica charantia and Calendula officinalis

    Directory of Open Access Journals (Sweden)

    T S Roopashree

    2008-09-01

    Full Text Available Summary: In view of increasing resistance to existing antimicrobial agents, herbal drugs are being looked as very importance source for discovery of new agents for treating various ailments related to bacterial infections. Cassia tora, Calendula officinalis and Momordica charantia are well known plants in Asia including India which posses wide range of pharmacological activities. These drugs have been used in India as  folk remedy in the form of decoctions and infusions to treat bacterial infections and also claimed to be an effective against variety of skin conditions like psoriasis, acne, wounds etc. The present investigation was carried out to study the unexplored area of these drugs towards their antibacterial activity with respect to their traditional use as antipsoriatic agents. The herbs were subjected to successive extraction using different solvents and the extracts were subjected to antibacterial evaluation against both gram positive and gram negative organisms by cup plate technique. Among the various extracts, aqueous extracts were found to be more effective against all the bacteria. Staphyllococcus aureus was more susceptible to the aqueous extracts among the tested organisms.   Industrial relevance: The selected herbs have been used traditionally for treating skin diseases like psoriasis, for which there is no complete cure till date. Though exact mechanism of these herbs for their effectiveness in psoriasis is not understood, according to American academy of dermatology antibacterial therapy could also be used for treating psoriasis. In view of this study of antibacterial activity was carried out. Among the various extracts, aqueous extracts exhibited highest activity especially against S. aureus which has been one of the organisms which aggravate conditions like psoriasis. As the aqueous extracts have shown excellent activity, these extracts could be formulated individually or in combination as external or internal dosage forms

  12. D-Glucose as a modifying agent in gelatin/collagen matrix and reservoir nanoparticles for Calendula officinalis delivery.

    Science.gov (United States)

    Lam, P-L; Kok, S H-L; Bian, Z-X; Lam, K-H; Tang, J C-O; Lee, K K-H; Gambari, R; Chui, C-H

    2014-05-01

    Gelatin/Collagen-based matrix and reservoir nanoparticles require crosslinkers to stabilize the formed nanosuspensions, considering that physical instability is the main challenge of nanoparticulate systems. The use of crosslinkers improves the physical integrity of nanoformulations under the-host environment. Aldehyde-based fixatives, such as formaldehyde and glutaraldehyde, have been widely applied to the crosslinking process of polymeric nanoparticles. However, their potential toxicity towards human beings has been demonstrated in many previous studies. In order to tackle this problem, D-glucose was used during nanoparticle formation to stabilize the gelatin/collagen-based matrix wall and reservoir wall for the deliveries of Calendula officinalis powder and oil, respectively. In addition, therapeutic selectivity between malignant and normal cells could be observed. The C. officinalis powder loaded nanoparticles significantly strengthened the anti-cancer effect towards human breast adenocarcinoma MCF7 cells and human hepatoma SKHep1 cells when compared with the free powder. On the contrary, the nanoparticles did not show significant cytotoxicity towards normal esophageal epithelial NE3 cells and human skin keratinocyte HaCaT cells. On the basis of these evidences, D-glucose modified gelatin/collagen matrix nanoparticles containing C. officinalis powder might be proposed as a safer alternative vehicle for anti-cancer treatments. PMID:24657927

  13. Structure and cytotoxic activity of sesquiterpene glycoside esters from Calendula officinalis L.: Studies on the conformation of viridiflorol.

    Science.gov (United States)

    D'Ambrosio, Michele; Ciocarlan, Alexandru; Colombo, Elisa; Guerriero, Antonio; Pizza, Cosimo; Sangiovanni, Enrico; Dell'Agli, Mario

    2015-09-01

    Topic applications of Calendula officinalis L. lipophilic extracts are used in phytotherapy to relieve skin inflammatory conditions whereas infusions are used as a remedy for gastric complaints. Such a different usage might be explained by some cytotoxicity of lipophilic extracts at gastric level but little is known about this. Therefore, we screened the CH2Cl2 extract from the flowers of C. officinalis by MTT and LDH assays in human epithelial gastric cells AGS. This bioassay-oriented approach led to the isolation of several sesquiterpene glycosides which were structurally characterized by spectroscopic measurements, chemical reactions and MM calculations. The conformational preferences of viridiflorol fucoside were established and a previously assigned stereochemistry was revised. The compounds 1a, 2a and 3f showed comparably high cytotoxicity in the MTT assays, whereas the effect on LDH release was lower. Our study provides new insights on the composition of C. officinalis extracts of medium polarity and identifies the main compounds that could be responsible for cytotoxic effects at gastric level. PMID:26057223

  14. Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis Utilização de técnicas de cultura de tecidos na proliferação de brotos e enraizamento de Calendula officinalis

    Directory of Open Access Journals (Sweden)

    Cristiane Pimentel Victório

    2012-09-01

    Full Text Available The high demand for plant material from Calendula officinalis in the production of herbal medicines and cosmetics, turns the technique of plant-tissue culture into one of the alternatives for the improvement of crops over a short period of time. A protocol for tissue culture was developed from segments of seedlings of C. officinalis, in order to improve the proliferation of shoots and roots. We used a Murashige and Skoog (MS½N medium, reduced to half the concentrations of NH4NO3 and KNO3 to verify the effect of different types of explants (basal, intermediate, and apical, a medium containing beach sand as support instead of agar, and the effect of auxins and cytokinins (TDZ tidiazuron; BAP, 6-benzylaminopurine, IAA, indol-3-acetic acid, IBA, indol-3-butyric acid, NAA, naphthalene-acetic acid on plant development in vitro. The results showed pronounced rooting from the apical explants, as well as a greater elongation of shoots and number of leaves. The solid medium was more suitable for the C. officinalis cultures. Shoot proliferation was dependent on cytokinins with better results from the influence of TDZ or BAP compared to the other treatments. Plants regenerated from the medium containing TDZ displayed a glazed appearance and morphogenetic deformations. The highest rate for rooting (80% was obtained using IAA 0.1mgL-1. Through in-vitro propagation, healthy C. officinalis plants were obtained with roots which can acclimatise, allowing the continuous supplement of raw material.A alta demanda por material vegetal de Calendula officinalis para produção de fitoterápicos e cosméticos, configura a técnica de cultura de tecidos vegetais como uma das alternativas para o aprimoramento das culturas em curto período de tempo. Um protocolo de cultura de tecidos foi desenvolvido a partir de segmentos de plântulas de C. officinalis, no intuito de melhorar a proliferação de brotos e o enraizamento. Foi utilizado o meio Murashige e Skoog (MS

  15. Fotosíntesis, crecimiento y aceites esenciales en morfotipos florales de caléndula (Calendula officinalis L.).

    OpenAIRE

    Salomé Abarca, Luis Francisco

    2014-01-01

    La caléndula (Calendula officinalis) es una especie herbácea originaria de Egipto, que se cree fue introducida a Europa en el siglo XII. En México es cultivada como una planta medicinal y de ornato, en lugares con clima semiseco y templado. En la zona de los valles centrales de México, Palma observó amplia diversidad fenotípica en una variedad común de caléndula, y eligió seis variantes en la morfología floral de esa población heterogénea, a las cuales denominó de la S1 a la S6. El objetivo ...

  16. Pharmacodynamic Research and Safety Evaluation of Calendula Officinalis L.%金盏菊的安全性评价及药效学研究

    Institute of Scientific and Technical Information of China (English)

    郑佳; 卢先明; 徐利; 于宗强

    2013-01-01

    Objective: To study the pharmacodynamic of Calendula officinalis L and to evaluate its safety. Methods: The safety evaluation of Calendula officinalis L was evaluated by maximum tolerance of mice. The effects of Calendula officinali L. on bleeding time and blood coagulation time in mice were observed through the methods of tail cutting and blood coagulation in glass capillary and anti - inflammatory effect was investigated by xylene - induced mice ear swelling test Results: The maximum tolerance dose to mice was 80 g/kg, which was equal to 320 times of aducts'maximum oral dose per day. The bleeding time was shortened significantly by the high dosage of Calendula officinalis L decocting; the blood coagulation time was also shortened significantly by high dosage, middle dosage and low dosage of Calendula officinalis L decocting, and Calendula officinalis L decocting has certain trend on anti - inflammatory effect, but it did not have statistical significance. Conclusion: There was no any acute toxic reaction of Calendula officinalis L decocting and the security is very well It has the effect of hemostasis and has a certain anti - inflammatory effect The renewable resources of Calendula officinalis L are rich and it is worthy to be developed and utilized.%目的:对金盏菊的药效学进行初步研究,并对其安全性做出评价.方法:采用最大耐受量测定法对金盏菊进行安全性评价;分别采用剪尾法、毛细玻璃管法、二甲苯致小鼠耳肿胀法观察金盏菊对小鼠出血时间、凝血时间的影响及抗炎作用.结果:小鼠最大耐受量为80g/kg,为成人每日最大用量的320倍.金盏菊水煎液高剂量组能明显缩短小鼠的出血时间(P<0.05);金盏菊水煎液高剂量组、中剂量组、低剂量组均能明显缩短小鼠的凝血时间(P<0.05);金盏菊水煎液对二甲苯致小鼠耳肿胀有一定的抗炎趋势,但不具备统计学意义.结论:金盏菊水煎液安全性好,有良好的止

  17. Padronização da tintura de Calendula officinalis L. para seu emprego em formulações semi-sólidas fitoterápicas

    OpenAIRE

    Nunes, Kariane M.; Barbosa, Wagner L.R.; Ozela, Eliana F.; Silva Júnior, José O. C.

    2009-01-01

    Apesar da extensa utilização dos fitoterápicos, a qualidade destes medicamentos muitas vezes é deficiente e questionável. A tintura de Calendula officinalis L. tem um vasto emprego na obtenção de formulações fitoterápicas semi-sólidas, por seus efeitos antiinflamatórios, antisépticos e cicatrizantes. Sendo assim, o presente estudo objetivou a aplicação de metodologias estabelecidas pela legislação brasileira para a caracterização e controle de qualidade do pó e tintura de C. officinalis, visa...

  18. Antimicrobial activity of Calendula officinalis, Camellia sinensis and chlorhexidine against the adherence of microorganisms to sutures after extraction of unerupted third molars

    OpenAIRE

    FARIA, Raquel Lourdes; CARDOSO, Lincoln Marcelo Lourenço; Akisue, Gokithi; PEREIRA, Cristiane Aparecida; Junqueira, Juliana Campos; Jorge, Antonio Olavo Cardoso; SANTOS JÚNIOR, Paulo Villela

    2011-01-01

    Objective The objective of this study was to compare the antimicrobial effect of mouthwashes containing Calendula officinalis L., Camellia sinensis (L.) Kuntze and 0.12% chlorhexidine digluconate on the adherence of microorganisms to suture materials after extraction of unerupted third molars. Material and Methods Eighteen patients with unerupted maxillary third molars indicated for extraction were selected (n=6 per mouthwash). First, the patients were subjected to extraction of the left toot...

  19. COMPARATIVE ANALYSIS OF THE USE OF CMR POMADE (BELLISPERENIS + CALENDULA OFFICINALIS + MYRISTICASEBIFERA) AND PHYSIOLOGICAL SOLUTION IN TISSUE SCARRING OF RATS (RATHUSNORVEGICUS)

    OpenAIRE

    Fernandes, E S; R. de A. Santos; B. S. Oliveira; D. S. Meireles

    2014-01-01

    Pharmaceutical market has several types of ointments that are used in animals for healing processes, arising from traumatic injuries or surgical procedures. The use of homeopathic medicines in the treatment of skin wounds has grown significantly, but no one knows for sure the efficiency of such drugs. Thus, this paper aimed to measure the drug's effectiveness CMR (Bellisperenis + Calendula officinalis Myristica sebifera) in comparison to physiologic solution in treating skin wounds produced i...

  20. Evaluation of in vitro anticancer activity of Ocimum basilicum, Alhagi maurorum, Calendula officinalis and their parasite Cuscuta campestris.

    Science.gov (United States)

    Behbahani, Mandana

    2014-01-01

    The present investigation was carried out to study the relationship between presence of cytotoxic compounds in Ocimum basilicum, Alhagi maurorum, Calendula officinalis and their parasite Cuscuta campestris. The cytotoxic activity of the pure compounds was performed by MTT assay against breast cancer cell lines (MCF-7 and MDA-MB-231) and normal breast cell line (MCF 10A). The induction of apoptosis was measured by the expression levels of p53, bcl-2, bax and caspase-3 genes using quantitative Real Time PCR. Three active fractions were detected by nuclear magnetic resonance as lutein, lupeol and eugenol, respectively, in C. officinalis, A. maurorum and O. basilicum. These compounds and their epoxidized forms were also detected in their parasite C. campestris. The cytotoxic activity of lutein epoxide, lupeol epoxide and eugenol epoxide was significantly more than lutein, lupeol and eugenol. The mRNA expression level of p53, caspase-3 and bax genes were increased in both cancer cells treated with all pure compounds. However, bcl-2 gene expression decreased in treated breast cancer cells. In conclusion, all the data indicated that the epoxide forms of lupeol, lutein and eugenol are potential drug candidates for inducing apoptosis in human breast cancer cells. PMID:25548920

  1. In vitro acaricidal activity of ethanolic and aqueous floral extracts of Calendula officinalis against synthetic pyrethroid resistant Rhipicephalus (Boophilus) microplus.

    Science.gov (United States)

    Godara, R; Katoch, R; Yadav, A; Ahanger, R R; Bhutyal, A D S; Verma, P K; Katoch, M; Dutta, S; Nisa, F; Singh, N K

    2015-09-01

    Detection of resistance levels against deltamethrin and cypermethrin in Rhipicephalus (Boophilus) microplus collected from Jammu (India) was carried out using larval packet test (LPT). The results showed the presence of resistance level II and I against deltamethrin and cypermethrin, respectively. Adult immersion test (AIT) and LPT were used to evaluate the in vitro efficacy of ethanolic and aqueous floral extracts of Calendula officinalis against synthetic pyrethroid resistant adults and larvae of R. (B.) microplus. Four concentrations (1.25, 2.5, 5 and 10 %) of each extract with four replications for each concentration were used in both the bioassays. A concentration dependent mortality was observed and it was more marked with ethanolic extract. In AIT, the LC50 values for ethanolic and aqueous extracts were calculated as 9.9 and 12.9 %, respectively. The egg weight of the live ticks treated with different concentrations of the ethanolic and aqueous extracts was significantly lower than that of control ticks; consequently, the reproductive index and the percent inhibition of oviposition values of the treated ticks were reduced. The complete inhibition of hatching was recorded at 10 % of ethanolic extract. The 10 % extracts caused 100 % mortality of larvae after 24 h. In LPT, the LC50 values for ethanolic and aqueous extracts were determined to be 2.6 and 3.2 %, respectively. It can be concluded that the ethanolic extract of C. officinalis had better acaricidal properties against adults and larvae of R. (B.) microplus than the aqueous extract. PMID:26071101

  2. Evaluation of in vitro anticancer activity of Ocimum basilicum, Alhagi maurorum, Calendula officinalis and their parasite Cuscuta campestris.

    Directory of Open Access Journals (Sweden)

    Mandana Behbahani

    Full Text Available The present investigation was carried out to study the relationship between presence of cytotoxic compounds in Ocimum basilicum, Alhagi maurorum, Calendula officinalis and their parasite Cuscuta campestris. The cytotoxic activity of the pure compounds was performed by MTT assay against breast cancer cell lines (MCF-7 and MDA-MB-231 and normal breast cell line (MCF 10A. The induction of apoptosis was measured by the expression levels of p53, bcl-2, bax and caspase-3 genes using quantitative Real Time PCR. Three active fractions were detected by nuclear magnetic resonance as lutein, lupeol and eugenol, respectively, in C. officinalis, A. maurorum and O. basilicum. These compounds and their epoxidized forms were also detected in their parasite C. campestris. The cytotoxic activity of lutein epoxide, lupeol epoxide and eugenol epoxide was significantly more than lutein, lupeol and eugenol. The mRNA expression level of p53, caspase-3 and bax genes were increased in both cancer cells treated with all pure compounds. However, bcl-2 gene expression decreased in treated breast cancer cells. In conclusion, all the data indicated that the epoxide forms of lupeol, lutein and eugenol are potential drug candidates for inducing apoptosis in human breast cancer cells.

  3. Mutagenic influences of colchicine on phenological and molecular diversity of Calendula officinalis L.

    Science.gov (United States)

    El-Nashar, Y I; Ammar, M H

    2016-01-01

    Six different colchicine concentrations: 0, 400, 800, 1200, 1600, and 2000 ppm, in combination with four soaking time treatments (1, 2, 3, and 4 h), were selected to assess the effects on germination, vegetative growth, and flower yield components in calendula plants. The molecular diversity among the treatments was assessed using ten SRAP marker combinations. Seed soaking in colchicine significantly enhanced both the fresh and the dry shoot and root masses, flowering date, number of flowers per plant, and flower diameter. At 1200-ppm colchicine combined with a 4-h soaking time, a superior effect on seed germination was observed, whereas 800 ppm for 4 h produced the highest number of flowers and the largest flower diameter. The earliest flowering time was found at 800 ppm combined with a short soaking time (1 h), while the 4-h soaking time with 800 ppm, is recommended for growing calendula outdoors, since it enhances flower development. At the molecular level, 752 fragments were successfully amplified using the SRAP primers, with 280 genetic loci found throughout the calendula genome. The polymorphism percentage ranged from 79 to 100% and the polymorphic information content (PIC) values ranged between 0.85 and 0.97. The high number of detected loci and PIC values suggests a great power of SRAP markers in detecting mutant molecular diversity. Our results clearly show the existence of genetic variation among colchicine treated calendula plants and the clustering of the studied mutants was concordant with the colchicine concentration used. PMID:27173261

  4. Assessment of plant development, morphology and flavonoid content in different cultivation treatments of Calendula officinalis L: , Asteraceae

    Directory of Open Access Journals (Sweden)

    Luis Fernando Nunes Alves Paim

    2010-12-01

    Full Text Available The objective of this study was to examine the influence of cultivation treatments on plant development, morphology and flavonoid content in Calendula officinalis L., Asteraceae. The cultivation treatments were liming (LIM, liming + organic fertilizer (LORG and liming + NPK chemical fertilizer (LCHEM. The experiment was completely randomized with sixty repetitions and the means were compared by ANOVA and Duncan's test (significance of 95%. The results show that LORG and LCHEM produced greater plant development compared with LIM treatment. The LORG treatment also produced plants with greater homogeneity, fresh mass, and higher numbers of flowers per plant, but lower biomass of flower produced by each individual. Morphoanatomical variations were also observed with tubular flowers with yellow centres (TCA and tubular flowers with brown centres (TAC that produced a higher content of flavonoids (1.41 and 1.44% respectively, compared with ligulate flowers with yellow centres (LCA and ligulate flowers with brown centres (LAC (0.89 and 0.95% respectively. The chemical compounds in the flowers of calendula viewed by thin-layer chromatography showed that the treatments did not have any influence on the qualitative chemical profile of the plant. However, cultivars of calendula that underwent treatment LCHEM produced a greater quantity of total flavonoids (1.14% against 1.04% for LORG and 1.03% for LIM.O objetivo deste estudo foi avaliar a influência dos tratos culturais no desenvolvimento vegetal, morfologia e teor de flavonóides para Calendula officinalis L., Asteraceae. Os tratos culturais foram: calagem (CA, calagem + adubação orgânica (CAO e calagem + adubação química NPK (CAQ. O experimento foi inteiramente casualizado com sessenta repetições e a comparação das médias realizada por ANOVA e teste de Duncan (significância de 95%. Os resultados apontam que CAO e CAQ apresentaram maior desenvolvimento vegetal comparados com tratamento CA. O

  5. Optimization of VIGS and functional analysis of Gerbera hybrida TCP genes in Calendula officinalis

    OpenAIRE

    Zhang, Yao

    2012-01-01

    The evolution of floral novelties involves activities of several transcription factors. The large Asteraceae plant family has inflorescences consisting of different types of flowers. TCP transcription factors have recently been reported to regulate this complex structure. Gerbera hybrida, a common ornamental crop, has been used as a model plant for flower developmental studies in Asteraceae. It can be genetically transformed; however, transformation is laborious and time-consuming. Calendula ...

  6. Antioxidant capacity of calendula officinalis flowers extract and prevention of radiation induced oropharyngeal mucositis in patients with head and neck cancers: a randomized controlled clinical study.

    Science.gov (United States)

    Babaee, Neda; Moslemi, Dariush; Khalilpour, Mohammad; Vejdani, Fatemeh; Moghadamnia, Yasaman; Bijani, Ali; Baradaran, Mahmoud; Kazemi, Mohammad Taghi; Khalilpour, Asieh; Pouramir, Mahdi; Moghadamnia, Ali Akbar

    2013-01-01

    This study was designed to determine the effect of Calendula officinalis flowers extract mouthwash as oral gel on radiation-induced oropharyngeal mucositis (OM) in patients with head-and-neck cancer. Forty patients with neck and head cancers under radiotherapy or concurrent chemoradiotherapy protocols were randomly assigned to receive either 2% calendula extract mouthwash or placebo (20 patients in each group). Patients were treated with telecobalt radiotherapy at conventional fractionation (200 cGy/fraction, five fractions weekly, 30-35 fractions within 4-7 weeks). The oropharyngeal mucositis was evaluated by two clinical investigators (a radiation oncologist and a dentist), using the oral mucositis assessment scale (OMAS). Trying to find out the possible mechanism of action of the treatment, total antioxidant, polyphenol and flavonoid contents, and quercetin concentration of the mouth wash were measured. Calendula mouthwash significantly decreased the intensity of OM compared to placebo at week 2 (score: 5.5 vs. 6.8, p = 0.019), week 3 (score: 8.25 vs. 10.95, p Calendula extract gel could be effective on decreasing the intensity of radiotherapy- induced OM during the treatment and antioxidant capacity may be partly responsible for the effect. PMID:23497687

  7. Qualitative and quantitative high performance thin layer chromatography analysis of Calendula officinalis using high resolution plate imaging and artificial neural network data modelling.

    Science.gov (United States)

    Agatonovic-Kustrin, S; Loescher, Christine M

    2013-10-10

    Calendula officinalis, commonly known Marigold, has been traditionally used for its anti-inflammatory effects. The aim of this study was to investigate the capacity of an artificial neural network (ANN) to analyse thin layer chromatography (TLC) chromatograms as fingerprint patterns for quantitative estimation of chlorogenic acid, caffeic acid and rutin in Calendula plant extracts. By applying samples with different weight ratios of marker compounds to the system, a database of chromatograms was constructed. A hundred and one signal intensities in each of the HPTLC chromatograms were correlated to the amounts of applied chlorogenic acid, caffeic acid, and rutin using an ANN. The developed ANN correlation was used to quantify the amounts of 3 marker compounds in calendula plant extracts. The minimum quantifiable level (MQL) of 610, 190 and 940 ng and the limit of detection (LD) of 183, 57 and 282 ng were established for chlorogenic, caffeic acid and rutin, respectively. A novel method for quality control of herbal products, based on HPTLC separation, high resolution digital plate imaging and ANN data analysis has been developed. The proposed method can be adopted for routine evaluation of the phytochemical variability in calendula extracts. PMID:24070490

  8. Analysis of the essential oils from Calendula officinalis growing in Brazil using three different extraction procedures Análise por CG-EM do óleo essencial de Calendula officinalis cultivado no Brasil utilizando-se três diferentes processos de extração

    OpenAIRE

    Zilda Cristina Gazim; Claudia Moraes Rezende; Sandra Regina Fraga; Benedito Prado Dias Filho; Celso Vataru Nakamura; Diógenes Aparicio Garcia Cortez

    2008-01-01

    Terpenes and aroma volatiles from flowers of Calendula officinalis cultivated in southeastern Brazil were obtained by steam distillation (SD), headspace-cold finger (HS-CF) extraction and headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography and mass spectrometric analysis. The dried flowers contained 0.1% oil. Kovats indices and mass spectra were used to identify 27 individual components in the various volatile fractions. The main components present in the volatile ...

  9. Effect of Arbuscular Mycorrhizal Fungi On Yield and Phytoremediation Performance of Pot Marigold (Calendula officinalis L.) Under Heavy Metals Stress.

    Science.gov (United States)

    Tabrizi, Leila; Mohammadi, Siavash; Delshad, Mojtaba; Moteshare Zadeh, Babak

    2015-01-01

    In order to study the effect of mycorrhizal fungi (inoculated and non-inoculated) and heavy metals stress [0, Pb (150 and 300 mg/kg) and Cd (40 and 80 mg/kg)] on pot marigold (Calendula officinalis L.), a factorial experiment was conducted based on a randomized complete block design with 4 replications in Research Greenhouse of Department of Horticultural Sciences, University of Tehran, Iran, during 2012-2013. Plant height, herbal and flower fresh and dry weight, root fresh and dry weight and root volume, colonization percentage, total petal extract, total petal flavonoids, root and shoot P and K uptakes, and Pb and Cd accumulations in root and shoot were measured. Results indicated that with increasing soil Pb and Cd concentration, growth and yield of pot marigold was reduced significantly; Cd had greater negative impacts than Pb. However, mycorrhizal fungi alleviated these impacts by improving plant growth and yield. Pot marigold concentrated high amounts of Pb and especially Cd in its roots and shoots; mycorrhizal plants had a greater accumulation of these metals, so that those under 80 mg/kg Cd soil(-1) accumulated 833.3 and 1585.8 mg Cd in their shoots and roots, respectively. In conclusion, mycorrhizal fungi can improve not only growth and yield of pot marigold in heavy metal stressed condition, but also phytoremediation performance by increasing heavy metals accumulation in the plant organs. PMID:26237494

  10. Protective Effect of Calendula officinalis L. Flowers Against Monosodium Glutamate Induced Oxidative Stress and Excitotoxic Brain Damage in Rats.

    Science.gov (United States)

    Shivasharan, B D; Nagakannan, P; Thippeswamy, B S; Veerapur, V P

    2013-07-01

    Monosodium glutamate (MSG) is a popular flavour enhancer used in food industries; however, excess MSG is neurotoxic. Oxidative stress is well documented in MSG induced neurotoxicity. The compounds having antioxidant and anti-inflammatory properties reportedly possess beneficial effects against various neurotoxic insults. Calendula officinalis Linn. flower extract (COE) is known for its potent antioxidant and anti-inflammatory activities. Hence, this present study has been designed to evaluate the neuroprotective effect of COE on MSG-induced neurotoxicity in rats. Adult Wistar rats were administered systemically for 7 days with MSG and after one h of MSG injection, rats were treated with COE (100 and 200 mg/kg) orally. At the end the treatment period, animals were assessed for locomotor activity and were sacrificed; brains were isolated for estimation of LPO, GSH, CAT, TT, GST, Nitrite and histopathological studies. MSG caused a significant alteration in animal behavior, oxidative defense (raised levels of LPO, nitrite concentration, depletion of antioxidant levels) and hippocampal neuronal histology. Treatment with COE significantly attenuated behavioral alterations, oxidative stress, and hippocampal damage in MSG-treated animals. Hence, this study demonstrates that COE protects against MSG-induced neurotoxicity in rats. The antioxidant and anti-inflammatory properties of COE may be responsible for its observed neuroprotective action. PMID:24426226

  11. Light Absorption and Carotenoid Synthesis of Pot Marigold (Calendula officinalis L. in Response to Phosphorous and Potassium Varying Levels

    Directory of Open Access Journals (Sweden)

    Mohammad SEDGHI

    2011-03-01

    Full Text Available In order to provide additional information on the effects of elemental deficiency on factors that affect plant production in medicinal plants, a factorial field experiment as randomized complete block design was conducted on Calendula officinalis. Treatments were four phosphorus levels (P2O5 including 0, 40, 80 and 120 Kg ha-1 and four potassium levels (K2O as 0, 50, 100 and 150 Kg ha-1. Results showed that applied treatments had significant effects on petal carotenoids and the highest amount of ? - carotene obtained at 80 and 150 Kg ha-1 P2O5 and K2O, respectively. Effect of K2O on light interception and light use efficiency was significant and the highest radiation use efficiency achieved by applying 150 Kg ha-1 K2O. The highest yield of grain and dry flowers was recorded in the mixture of 80 and 150 Kg ha-1 P2O5 and K2O, respectively. In conclusion, using of K2O had greater effects on studied traits than P2O5.

  12. Stimulation or inhibition of beta-carotene's biosynthesis by irradiation with γ-rays of Calendula Officinalis seeds

    International Nuclear Information System (INIS)

    It is important to see what is the effect of irradiation with gamma rays of Calendula officinalis (marigolds) seeds. Could it be a stimulation or a inhibition of beta-carotene's biosynthesis? This paper presents results of irradiation of germinated seeds of marigolds. It was carried out irradiation of seeds at 8 different doses ( 1 kRad, 3.3 kRad, 10 kRad, 25 kRad, 50 kRad, 100 kRad, 200 kRad, 500 krad), one set of seeds being unirradiated for comparison. The plants had been grown in the same conditions of light, temperature and humidity. The identification of β-carotene was realized through thin layer chromatography (TLC). The chemical concentrations of β-carotene in leaves and stalks of marigolds had been determined by Varian spectrophotometer. Quantitative determination of β-carotene was carried out through spectral analysis using a standard spectrum of carotenoids and chlorophyll a and b. (authors)

  13. An investigation of influence of solvent on the degradation kinetics of carotenoids in oil extracts of Calendula officinalis

    Directory of Open Access Journals (Sweden)

    DEJAN BEZBRADICA

    2005-02-01

    Full Text Available The stability of carotenoids was studied in marigold oil extracts prepared with following solvents: Myritol 312®, paraffin oil, almond oil, olive oil, sunflower oil, grape seed oil, and soybean oil. The concentration of the carotenoids was determined by spectroscopic measurement at 450 nm. Degradation rate showed a first order dependence on the concentration of carotenoids with a faster first stage (which lasted 35–50 days, depending on the solvent and a slower second stage. The highest degradation rates were observed in extracts prepared with linoleic acid rich solvents (sunflower oil, soybean oil and grape seed oil, while the lowest were found in oil with saturated fatty acids (Myritol 312® and paraffin oil. These results confirm the connection between the degradation of carotenoids and lipid autoxidation, and suggest that the influence of the oil solvents on the stability of oil extracts of Calendula officinalis is a factor that must be considered when selecting a solvent for the production of marigold oil extracts.

  14. The effect of different nitrogen fertilization rates on yield and quality of marigold (Calendula officinalis L. 'Tokaj' raw material

    Directory of Open Access Journals (Sweden)

    Beata Król

    2012-12-01

    Full Text Available Pot marigold (Calendula officinalis L. is an annual ornamental plant which is also grown for herbal raw material (flower heads used in the pharmaceutical and cosmetic industries. A field experiment was carried out in the years 2006-2008 in the Experimental Farm of the University of Life Sciences in Lublin. The study was conducted on loess soil with the granulometric composition of silt loam. The aim of the experiment was to determine the effect of different nitrogen rates (0, 40, 80, 120, 160 kg N × ha-1 on some morphological features of flower heads as well as on yield and quality of pot marigold raw material. Flowering of pot marigold was shortest in the control treatment (32 days and longest (43 days in the plot where nitrogen fertilization had been applied at the highest rate (160 kg N × ha-1. Nitrogen fertilization had a significant influence on the number of flower heads per plant, but no significant difference was found in diameter as well as in ligulate flowers and tubular flowers in the flower head. It was found to increase significantly raw material yield after the application of 80 kg N × ha-1, compared to the control treatment. Yield of flower heads did not differ markedly for fertilization rates from 80 to 160 kg N × ha-1. Nitrogen fertilization modified slightly essential oil content (this content increased with increasing nitrogen rates, but at the same time it decreased the percentage of flavonoid compounds.

  15. A Bio-Guided Fractionation to Assess the Inhibitory Activity of Calendula officinalis L. on the NF-κB Driven Transcription in Human Gastric Epithelial Cells.

    Science.gov (United States)

    Colombo, Elisa; Sangiovanni, Enrico; D'Ambrosio, Michele; Bosisio, Enrica; Ciocarlan, Alexandru; Fumagalli, Marco; Guerriero, Antonio; Harghel, Petru; Dell'Agli, Mario

    2015-01-01

    Calendula officinalis L. has been largely known for its topical anti-inflammatory properties; however, there are no experimental evidences about its antiphlogistic effect at the gastric level. To investigate whether marigold might exert an activity against gastric inflammation, a CH2Cl2 extract obtained from C. officinalis flowers was evaluated in vitro on the NF-κB pathway. The lipophilic extract demonstrated a significant inhibitory effect on the NF-κB driven transcription. The identification of active compounds was conducted by a bio-guided fractionation of the extract that afforded 16 fractions. Fraction J exhibited a concentration-dependent inhibitory activity on the NF-κB driven transcription and significantly contributed to the antiphlogistic effect showed by CH2Cl2 extract. The main components of fraction J were loliolide and the fucoside acetates of β-eudesmol and viridiflorol. HPLC analysis of fractions D and E led to the identification and isolation of triterpene esters that showed a concentration-dependent inhibition of the NF-κB driven transcription, with faradiol-3-myristate and the corresponding aglycone being the most active compounds. The present study provides some experimental evidences that Calendula officinalis L. may exert an anti-inflammatory activity on the gastric district by the inhibition of the NF-κB system, identifying the compounds responsible, at least in part, for the observed effect. PMID:26491463

  16. A Bio-Guided Fractionation to Assess the Inhibitory Activity of Calendula officinalis L. on the NF-κB Driven Transcription in Human Gastric Epithelial Cells

    Directory of Open Access Journals (Sweden)

    Elisa Colombo

    2015-01-01

    Full Text Available Calendula officinalis L. has been largely known for its topical anti-inflammatory properties; however, there are no experimental evidences about its antiphlogistic effect at the gastric level. To investigate whether marigold might exert an activity against gastric inflammation, a CH2Cl2 extract obtained from C. officinalis flowers was evaluated in vitro on the NF-κB pathway. The lipophilic extract demonstrated a significant inhibitory effect on the NF-κB driven transcription. The identification of active compounds was conducted by a bio-guided fractionation of the extract that afforded 16 fractions. Fraction J exhibited a concentration-dependent inhibitory activity on the NF-κB driven transcription and significantly contributed to the antiphlogistic effect showed by CH2Cl2 extract. The main components of fraction J were loliolide and the fucoside acetates of β-eudesmol and viridiflorol. HPLC analysis of fractions D and E led to the identification and isolation of triterpene esters that showed a concentration-dependent inhibition of the NF-κB driven transcription, with faradiol-3-myristate and the corresponding aglycone being the most active compounds. The present study provides some experimental evidences that Calendula officinalis L. may exert an anti-inflammatory activity on the gastric district by the inhibition of the NF-κB system, identifying the compounds responsible, at least in part, for the observed effect.

  17. A new extract of the plant calendula officinalis produces a dual in vitro effect: cytotoxic anti-tumor activity and lymphocyte activation

    International Nuclear Information System (INIS)

    Phytopharmacological studies of different Calendula extracts have shown anti-inflamatory, anti-viral and anti-genotoxic properties of therapeutic interest. In this study, we evaluated the in vitro cytotoxic anti-tumor and immunomodulatory activities and in vivo anti-tumor effect of Laser Activated Calendula Extract (LACE), a novel extract of the plant Calendula Officinalis (Asteraceae). An aqueous extract of Calendula Officinalis was obtained by a novel extraction method in order to measure its anti-tumor and immunomodulatory activities in vitro. Tumor cell lines derived from leukemias, melanomas, fibrosarcomas and cancers of breast, prostate, cervix, lung, pancreas and colorectal were used and tumor cell proliferation in vitro was measured by BrdU incorporation and viable cell count. Effect of LACE on human peripheral blood lymphocyte (PBL) proliferation in vitro was also analyzed. Studies of cell cycle and apoptosis were performed in LACE-treated cells. In vivo anti-tumor activity was evaluated in nude mice bearing subcutaneously human Ando-2 melanoma cells. The LACE extract showed a potent in vitro inhibition of tumor cell proliferation when tested on a wide variety of human and murine tumor cell lines. The inhibition ranged from 70 to 100%. Mechanisms of inhibition were identified as cell cycle arrest in G0/G1 phase and Caspase-3-induced apoptosis. Interestingly, the same extract showed an opposite effect when tested on PBLs and NKL cell line, in which in vitro induction of proliferation and activation of these cells was observed. The intraperitoneal injection or oral administration of LACE extract in nude mice inhibits in vivo tumor growth of Ando-2 melanoma cells and prolongs the survival day of the mice. These results indicate that LACE aqueous extract has two complementary activities in vitro with potential anti-tumor therapeutic effect: cytotoxic tumor cell activity and lymphocyte activation. The LACE extract presented in vivo anti-tumoral activity in nude

  18. Antimicrobial activity of Calendula officinalis, Camellia sinensis and chlorhexidine against the adherence of microorganisms to sutures after extraction of unerupted third molars

    Directory of Open Access Journals (Sweden)

    Raquel Lourdes Faria

    2011-10-01

    Full Text Available OBJECTIVE: The objective of this study was to compare the antimicrobial effect of mouthwashes containing Calendula officinalis L., Camellia sinensis (L. Kuntze and 0.12% chlorhexidine digluconate on the adherence of microorganisms to suture materials after extraction of unerupted third molars. MATERIAL AND METHODS: Eighteen patients with unerupted maxillary third molars indicated for extraction were selected (n=6 per mouthwash. First, the patients were subjected to extraction of the left tooth and instructed not to use any type of antiseptic solution at the site of surgery (control group. After 15 days, the right tooth was extracted and the patients were instructed to use the Calendula officinalis, Camellia sinensis or chlorhexidine mouthwash during 1 week (experimental group. For each surgery, the sutures were removed on postoperative day 7 and placed in sterile phosphate-buffered saline. Next, serial dilutions were prepared and seeded onto different culture media for the growth of the following microorganisms: blood agar for total microorganism growth; Mitis Salivarius bacitracin sucrose agar for mutans group streptococci; mannitol agar for Staphylococcus spp.; MacConkey agar for enterobacteria and Pseudomonas spp., and Sabouraud dextrose agar containing chloramphenicol for Candida spp. The plates were incubated during 24-48 h at 37ºC for microorganism count (CFU/mL. RESULTS: The three mouthwashes tested reduced the number of microorganisms adhered to the sutures compared to the control group. However, significant differences between the control and experimental groups were only observed for the mouthwash containing 0.12% chlorhexidine digluconate. CONCLUSIONS: Calendula officinalis L. and Camellia sinensis (L. Kuntze presented antimicrobial activity against the adherence of microorganisms to sutures but were not as efficient as chlorhexidine digluconate.

  19. Desenvolvimento de dentifrícios com extratos fluidos de CALENDULA OFFICINALIS l., (ASTERACEAE) e CASEARIA SYLVESTRIS Sw., (FLACOURTIACEAE),destinado ao tratamento de periodontias

    OpenAIRE

    Arantes, Angela Bonjorno

    2012-01-01

    Resumo: A Calendula officinalis L. (Asteraceae) e a Casearia sylvestris Sw. (Flacourtiaceae), são exemplares da flora medicinal que possuem propriedades terapêuticas destacandose as atividades antiinflamatória, antimicrobiana e cicatrizante, atribuídas ao sinergismo de seus constituintes químicos. No intuito de usufruir destes beneficios desenvolveu-se uma formulação dentifrícia, na forma gel, com seleção criteriosa de matérias-primas e excipientes que atendessem os requisitos farmacotécnicos...

  20. CHANGES IN THE ACTIVITY OF ANTIOXIDANT AND GLYOXYLATE CYCLE ENZYMES OF HYDRO-PRIMED CALENDULA OFFICINALIS (L.) SEEDS AFTER RE-DRYING TEMPERATURE STRESS

    OpenAIRE

    Mohammad SEDGHI

    2013-01-01

    In order to study the effect of re-drying temperatures on the enzymatic activation of hydro-primed Calendula officinalis (L.) seeds, a completely randomized design with three replications was conducted. Treatments were six different re-drying temperatures including control (without drying), 20, 30, 40, 50, and 60 ◦C applied after hydro-priming. Results showed that rapid drying at high temperatures (40-60 ◦C) caused a significant difference comparing control. The best re-drying temperature con...

  1. COMPARATIVE ANALYSIS OF THE USE OF CMR POMADE (BELLISPERENIS + CALENDULA OFFICINALIS + MYRISTICASEBIFERA AND PHYSIOLOGICAL SOLUTION IN TISSUE SCARRING OF RATS (RATHUSNORVEGICUS

    Directory of Open Access Journals (Sweden)

    E. S. Fernandes

    2014-09-01

    Full Text Available Pharmaceutical market has several types of ointments that are used in animals for healing processes, arising from traumatic injuries or surgical procedures. The use of homeopathic medicines in the treatment of skin wounds has grown significantly, but no one knows for sure the efficiency of such drugs. Thus, this paper aimed to measure the drug's effectiveness CMR (Bellisperenis + Calendula officinalis Myristica sebifera in comparison to physiologic solution in treating skin wounds produced in rats. Standardized in the back of the animals on both sides (right and left evaluating the healing potential of homeopathic ointment CMR compared to saline lesions were performed.

  2. Treatment of Bothrops alternatus envenomation by Curcuma longa and Calendula officinalis extracts and ar-turmerone Tratamento local do envenenamento por Bothrops alternatus com extrato de Curcuma longa e Calendula officinalis e ar-turmerone

    Directory of Open Access Journals (Sweden)

    M.M. Melo

    2005-02-01

    Full Text Available It was investigated the efficiency of two extracts of plants and one fraction of their properties against the local effects of bothropic envenomation. Bothrops alternatus venom (1.25µg diluted in 100µl of sterile saline solution was inoculated (intradermally into the shaved dorsal back skin of 30 New Zealand rabbits. The animals were divided in six groups receiving the following treatments: group I: subcutaneous application of Curcuma longa extract (1.0ml; group II: topic treatment of Curcuma longa hydroalcoholic extract (1.0ml; group III: topic application of ar-turmerone in vaseline (1.0g; group IV: topic application of Curcuma longa methanolic extract (1.0ml; group V: topic application of Calendula officinalis ointment (1.0g; group VI: topic application of saline (1.0ml. These treatments were done at 30 minutes, and at 2, 4, 24 and 72 hours after venom inoculation. Intensity of local edema, hemorrhagic halo and necrosis were evaluated until 168h after that. Additionally, seven days after the Bothrops venom inoculation, blood was collected from heart with and without EDTA (10% for hemogram and biochemical parameters (total protein, blood urea nitrogen, creatinine, and fibrinogen and all the animals were anesthetized, sacrificed by ether inhalation and submitted to necropsy. Fragments of tissues were taken for histopathological evaluation. The most efficient treatment for inhibition of edema, necrosis and local hemorrhage after Bothrops alternatus venom was the topic application of ar-turmerone.Investigou-se a eficácia do extrato de plantas no tratamento local do envenenamento botrópico. Veneno de serpentes Bothrops alternatus (1,25µg diluído em 100µl de solução salina estéril foi inoculado (via intradérmica entre as escápulas de 30 coelhos. Os animais foram divididos em seis grupos (tratamentos: grupo I: tratamento subcutâneo com extrato de Curcuma longa; grupo II: tratamento tópico com extrato hidroalcoólico de Curcuma longa

  3. Evaluation of Biologically Active Compounds from Calendula officinalis Flowers using Spectrophotometry

    OpenAIRE

    Butnariu Monica; Coradini Cristina

    2012-01-01

    Abstract Background This study aimed to quantify the active biological compounds in C. officinalis flowers. Based on the active principles and biological properties of marigolds flowers reported in the literature, we sought to obtain and characterize the molecular composition of extracts prepared using different solvents. The antioxidant capacities of extracts were assessed by using spectrophotometry to measure both absorbance of the colorimetric free radical scavenger 2,2-diphenyl-1-picrylhy...

  4. High performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC) for the qualitative and quantitative analysis of Calendula officinalis-advantages and limitations.

    Science.gov (United States)

    Loescher, Christine M; Morton, David W; Razic, Slavica; Agatonovic-Kustrin, Snezana

    2014-09-01

    Chromatography techniques such as HPTLC and HPLC are commonly used to produce a chemical fingerprint of a plant to allow identification and quantify the main constituents within the plant. The aims of this study were to compare HPTLC and HPLC, for qualitative and quantitative analysis of the major constituents of Calendula officinalis and to investigate the effect of different extraction techniques on the C. officinalis extract composition from different parts of the plant. The results found HPTLC to be effective for qualitative analysis, however, HPLC was found to be more accurate for quantitative analysis. A combination of the two methods may be useful in a quality control setting as it would allow rapid qualitative analysis of herbal material while maintaining accurate quantification of extract composition.

  5. High performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC) for the qualitative and quantitative analysis of Calendula officinalis-advantages and limitations.

    Science.gov (United States)

    Loescher, Christine M; Morton, David W; Razic, Slavica; Agatonovic-Kustrin, Snezana

    2014-09-01

    Chromatography techniques such as HPTLC and HPLC are commonly used to produce a chemical fingerprint of a plant to allow identification and quantify the main constituents within the plant. The aims of this study were to compare HPTLC and HPLC, for qualitative and quantitative analysis of the major constituents of Calendula officinalis and to investigate the effect of different extraction techniques on the C. officinalis extract composition from different parts of the plant. The results found HPTLC to be effective for qualitative analysis, however, HPLC was found to be more accurate for quantitative analysis. A combination of the two methods may be useful in a quality control setting as it would allow rapid qualitative analysis of herbal material while maintaining accurate quantification of extract composition. PMID:24880991

  6. Protective effect of Calendula officinalis Linn. flowers against 3-nitropropionic acid induced experimental Huntington's disease in rats.

    Science.gov (United States)

    Shivasharan, B D; Nagakannan, Pandian; Thippeswamy, Boreddy Shivanandappa; Veerapur, Veeresh Prabakar; Bansal, Punit; Unnikrishnan, Mazhuvancherry K

    2013-10-01

    Oxidative stress (OS) and nitric oxide mechanisms have been recently proposed in 3-nitropropionic acid (3-NP)-induced neurotoxicity. The compounds, having antioxidant, anti-inflammatory and estrogenic effects, have been suggested for neuroprotection in different experimental models. Calendula officinalis Linn. flower extract (COE) is known for its potent antioxidant, anti-inflammatory, estrogenic and neuroprotective activities. Hence, the present study was designed to evaluate the neuroprotective effect of COE on 3-NP-induced neurotoxicity in rats by observing behavioral changes, OS and striatal damage in rat brain. Adult female Wistar rats were pretreated with vehicle or COE (100 and 200 mg/kg) for 7 days, followed by cotreatment with 3-NP (15 mg/kg, intraperitoneally) for the next 7 days. At the end of the treatment schedule, rats were evaluated for alterations in sensory motor functions and short-term memory. Animals were sacrificed and brain homogenates were used for the estimation of lipid peroxidation (LPO), glutathione, total thiols, glutathione S-transferase, catalase and nitrite. A set of brain slices was used for the evaluation of neuronal damage in the striatal region of the brain. 3-NP caused significant alterations in animal behavior, oxidative defense system evidenced by raised levels of LPO and nitrite concentration, and depletion of antioxidant levels. It also produced a loss of neuronal cells in the striatal region. Treatment with COE significantly attenuated behavioral alterations, oxidative damage and striatal neuronal loss in 3-NP-treated animals. The present study shows that COE is protective against 3-NP-induced neurotoxicity in rats. The antioxidant, anti-inflammatory and estrogenic properties of COE may be responsible for its neuroprotective action. PMID:23590827

  7. Development of lamellar gel phase emulsion containing marigold oil (Calendula officinalis) as a potential modern wound dressing.

    Science.gov (United States)

    Okuma, C H; Andrade, T A M; Caetano, G F; Finci, L I; Maciel, N R; Topan, J F; Cefali, L C; Polizello, A C M; Carlo, T; Rogerio, A P; Spadaro, A C C; Isaac, V L B; Frade, M A C; Rocha-Filho, P A

    2015-04-25

    Appropriate therapeutics for wound treatments can be achieved by studying the pathophysiology of tissue repair. Here we develop formulations of lamellar gel phase (LGP) emulsions containing marigold (Calendula officinalis) oil, evaluating their stability and activity on experimental wound healing in rats. LGP emulsions were developed and evaluated based on a phase ternary diagram to select the best LGP emulsion, having a good amount of anisotropic structure and stability. The selected LGP formulation was analyzed according to the intrinsic and accelerated physical stability at different temperatures. In addition, in vitro and in vivo studies were carried out on wound healing rats as a model. The LGP emulsion (15.0% marigold oil; 10.0% of blend surfactants and 75.0% of purified water [w/w/w]) demonstrated good stability and high viscosity, suggesting longer contact of the formulation with the wound. No cytotoxic activity (50-1000 μg/mL) was observed in marigold oil. In the wound healing rat model, the LGP (15 mg/mL) showed an increase in the leukocyte recruitment to the wound at least on days 2 and 7, but reduced leukocyte recruitment after 14 and 21 days, as compared to the control. Additionally, collagen production was reduced in the LGP emulsion on days 2 and 7 and further accelerated the process of re-epithelialization of the wound itself. The methodology utilized in the present study has produced a potentially useful formulation for a stable LGP emulsion-containing marigold, which was able to improve the wound healing process. PMID:25684193

  8. EFFECTS OF PLANTING SPACE AND HARVEST TIME ON THE NUMBER, WEIGHT AND DIAMETER OF MARIGOLD (CALENDULA OFFICINALIS L. FLOWERS

    Directory of Open Access Journals (Sweden)

    Nada Parađiković

    2013-06-01

    Full Text Available The study was conducted during 2010 in marigold (Calendula officinalis L. to determine the effects of three plant densities (plant density A - 65 cm x 35 cm; plant density B - 65 cm x 25 cm; plant density C – 55 cm x 25 cm and harvest time on the number, weight and diameter of marigold flowers. The results showed that the plant density significantly influenced the number of flowers per plant and flower weight. The largest number of flowers per plant was recorded in the plant density B (13.2 and the lowest (9.87 in the plant density C. The lowest flower weight was recorded in the plant density C (1.31 g and was statistically lower than the flower weight in the plant densities A (1.42 g and B (1.38 g. The plant density significantly influenced the number of flowers on side branches, being the highest in the plant density B. The diameter of the marigold flower was not significantly influenced by the plant density. During the experiment, a total of 13 harvests were achieved. The greatest number of flowers per plant was harvested in the eighth, ninth and tenth harvest, while the largest flower weight was measured in the fifth and twelfth harvest. On the average, the number of flowers per plant / harvest was 11.63 and the weight of flowers was 1.38 g. Diameter of marigold flowers ranged from 2.89 cm to 3.59 cm in the thirteenth and the third harvest, respectively. The number of flowers on side branches per plant / harvest was 11.61.

  9. Optimization of chemometric approaches for the extraction of isorhamnetin-3-O-rutinoside from Calendula officinalis L.

    Science.gov (United States)

    Moraes, Maria Lourdes Leite; da Silva, Heron Dominguez Torres; Blanes, Lucas; Doble, Philip; Tavares, Marina Franco Maggi

    2016-06-01

    The application of Design of Experiments (DoE) to the determination of optimum conditions for an extraction process relies on the correct selection of mathematical models. The linear model is the one typically used; however, in some cases it does not always have superior performance, ignoring the real nature of the data and its appropriate descriptive model. In order to evaluate the extraction efficiency of isorhamnetin-3-O-rutinoside from flowers of Calendula officinalis L. a multivariate factorial analysis was used. Simulations were conducted using linear, quadratic, full cubic and special cubic models. A Simplex-Centroid design was chosen as it delivered greater precision with only minor errors versus other models tested. Analyses were performed by capillary zone electrophoresis using sodium tetraborate buffer (40mmolL(-1), pH 9.4) containing 10% methanol. The detection was linear over a range of 8.0-50.0mgL(-1) (r(2)=0.996), and the limits of detection (LOD) and quantification (LOQ) for isorhamnetin-3-O-rutinoside were 3.44mgL(-1) and 11.47mgL(-1), respectively. The full cubic model showed the best extraction results, with an error of 3.40% compared to analysis of variance, and a determination coefficient of 0.974. The difference between the responses at the reference point, calculated by the model, and the experimental response, varies around 2.72% for full cubic model. Comparison of the four models showed the full cubic model was the most appropriate one, allowing greater efficiency in the extraction of isorhamnetin-3-O-rutinoside. Selection of the model made it possible to obtain a 60% increase in sensitivity compared to the linear model. PMID:27131151

  10. Effects of Different Culture Medium on the Growth of Calendula officinalis%不同培养基质对金盏菊生长的影响研究

    Institute of Scientific and Technical Information of China (English)

    陈峰; 沈迎春; 刘阳; 李莉

    2014-01-01

    Using different culture medium,using pot Calendula officinalis as experimental material,research the effect of garden soil,mushroom residue,vermiculite and mixed of these three medium as culture medi-um on the growth and development of Calendula officinalis. The test results show that:the different culture medium containing nutrients difference;effect of different culture medium on the growth of Calendula offici-nalis is different;effect of different culture substrates on chlorophyll content,Catalase activity,superoxide dis-mutase(SOD)activity are different. Comprehensive morphological and physiological indexes on the growth of Calendula officinalis can be concluded:the mushroom residue as growth culture medium of Calendula offici-nalis is best.%采用不同的培养基质,利用盆栽金盏菊作为试验材料,研究田园土、菌渣、蛭石及三者的混合基质作为培养基对金盏菊生长发育的影响。结果表明:不同培养基质所含营养成分差别较大;不同培养基质对金盏菊生长发育影响不同;不同培养基质对金盏菊叶绿素含量、过氧化氢酶活性、超氧化物歧化酶(SOD)活性等影响均不同。综合金盏菊生长的形态指标和生理指标可知:用菌渣作为培养基质的金盏菊长势最好。

  11. Planting depth for oilseed calendula

    Science.gov (United States)

    Calendula (Calendula officinalis L.) is not only a popular ornamental plant in temperate climates, but also a potential oilseed crop. Its seed oil has high levels of calendic acid, which makes it a highly valued drying oil with important industrial applications. Much basic agronomic information on c...

  12. Evaluation of Biologically Active Compounds from Calendula officinalis Flowers using Spectrophotometry

    Directory of Open Access Journals (Sweden)

    Butnariu Monica

    2012-04-01

    Full Text Available Abstract Background This study aimed to quantify the active biological compounds in C. officinalis flowers. Based on the active principles and biological properties of marigolds flowers reported in the literature, we sought to obtain and characterize the molecular composition of extracts prepared using different solvents. The antioxidant capacities of extracts were assessed by using spectrophotometry to measure both absorbance of the colorimetric free radical scavenger 2,2-diphenyl-1-picrylhydrazyl (DPPH as well as the total antioxidant potential, using the ferric reducing power (FRAP assay. Results Spectrophotometric assays in the ultraviolet-visible (UV-VIS region enabled identification and characterization of the full range of phenolic and flavonoids acids, and high-performance liquid chromatography (HPLC was used to identify and quantify phenolic compounds (depending on the method of extraction. Methanol ensured more efficient extraction of flavonoids than the other solvents tested. Antioxidant activity in methanolic extracts was correlated with the polyphenol content. Conclusions The UV-VIS spectra of assimilator pigments (e.g. chlorophylls, polyphenols and flavonoids extracted from the C. officinalis flowers consisted in quantitative evaluation of compounds which absorb to wavelengths broader than 360 nm.

  13. Hepato and reno protective action of Calendula officinalis L. flower extract.

    Science.gov (United States)

    Preethi, Korengath Chandran; Kuttan, Ramadasan

    2009-03-01

    Flower extract of C. officinalis L. was evaluated for its protective effect against CCl4 induced acute hepatotoxicity and cisplatin induced nephrotoxicity. The activities of serum marker enzymes of liver injury like glutamate pyruvate transaminase (SGPT), glutamate oxaloacetate transaminase (SGOT) and alkaline phosphatase (ALP) which were increased by CCl4 injection was found to be significantly reduced by the pretreatment of the flower extract at 100 and 250 mg/kg body weight. The lipid peroxidation in liver, the marker of membrane damage and the total bilirubin content in serum were also found to be at significantly low level in the extract pretreated group, indicating its protective role. The kidney function markers like urea and creatinine were significantly increased in cisplatin treated animals. However, their levels were found to be lowered in the extract pretreated groups (100 and 250 mg/kg body weight). Moreover, cisplatin induced myelosuppression was ameliorated by the extract pretreatment. Treatment with the extract produced enhancement of antioxidant enzymes--superoxide dismutase and catalase and glutathione. Results suggest a protective role of the flower extract of C. officinalis against CCl4 induced acute hepatotoxicity and cisplatin induced nephrotoxicity. Extract has been found to contain several carotenoids of which lutein, zeaxanthin and lycopene predominates. Possible mechanism of action of the flower extract may be due to its antioxidant activity and reduction of oxygen radicals. PMID:19405380

  14. Antioxidant Capacity of Calendula Officinalis Flowers Extract and Prevention of Radiation Induced Oropharyngeal Mucositis in Patients with Head and Neck Cancers: A Randomized Controlled Clinical Study

    Directory of Open Access Journals (Sweden)

    Mohammad Taghi Kazemi

    2013-03-01

    Full Text Available This study was designed to determine the effect of Calendula officinalis flowers extract mouthwash as oral gel on radiation-induced oropharyngeal mucositis (OM in patients with head-and-neck cancer. Forty patients with neck and head cancers under radiotherapy or concurrent chemoradiotherapy protocols were randomly assigned to receive either 2% calendula extract mouthwash or placebo (20 patients in each group. Patients were treated with telecobalt radiotherapy at conventional fractionation (200 cGy/fraction, five fractions weekly, 30–35 fractions within 4–7 weeks. The oropharyngeal mucositis was evaluated by two clinical investigators (a radiation oncologist and a dentist, using the oral mucositis assessment scale (OMAS. Trying to find out the possible mechanism of action of the treatment, total antioxidant, polyphenol and flavonoid contents, and quercetin concentration of the mouth wash were measured. Calendula mouthwash significantly decreased the intensity of OM compared to placebo at week 2 (score: 5.5 vs. 6.8, p = 0.019, week 3 (score: 8.25 vs. 10.95, p < 0.0001 and week 6 (score: 11.4 vs. 13.35, p = 0.031. Total antioxidant, polyphenol and flavonoid contents and quercetin concentration of the 2% extract were 2353.4 ± 56.5 μM, 313.40 ± 6.52 mg/g, 76.66 ± 23.24 mg/g, and 19.41 ± 4.34 mg/l, respectively. Calendula extract gel could be effective on decreasing the intensity of radiotherapy- induced OM during the treatment and antioxidant capacity may be partly responsible for the effect.

  15. Analysis of the essential oils from Calendula officinalis growing in Brazil using three different extraction procedures Análise por CG-EM do óleo essencial de Calendula officinalis cultivado no Brasil utilizando-se três diferentes processos de extração

    Directory of Open Access Journals (Sweden)

    Zilda Cristina Gazim

    2008-09-01

    Full Text Available Terpenes and aroma volatiles from flowers of Calendula officinalis cultivated in southeastern Brazil were obtained by steam distillation (SD, headspace-cold finger (HS-CF extraction and headspace solid-phase microextraction (HS-SPME coupled with gas chromatography and mass spectrometric analysis. The dried flowers contained 0.1% oil. Kovats indices and mass spectra were used to identify 27 individual components in the various volatile fractions. The main components present in the volatile fractions of the C. officinalis flowers, obtained by SD, HS-SPME, and HS-CF, were δ-cadinene (22.5, 22.1, and 18.4 % and γ-cadinene (8.9, 25.4, and 24.9 % while 20.4 % of α-cadinol was seen only after SD extraction.Terpenos e aromas voláteis das flores de Calendula officinalis cultivados no sudoeste do Brasil foram isolados por arraste a vapor (SD, dedo frio (HS-CF e micro extração em fase sólida (HS-SPME acoplada à espectrometria de massas. As flores secas da C. officinalis contêm 0,1% de óleo essencial e foram identificadas 27 substâncias químicas através do cálculo do índice de Kováts e interpretação dos espectros de massas. As substâncias majoritárias presentes no óleo essencial das flores de C. officinalis, obtido por SD, HS-SPME e HS-CF foram δ-cadinene (22,5; 22,1 e 18,4 % γ-cadinene (8,9, 25,4 e 24,9 % e 20.4 % de α-cadinol foi observado apenas na extração por arraste a vapor.

  16. A new extract of the plant calendula officinalis produces a dual in vitro effect: cytotoxic anti-tumor activity and lymphocyte activation

    Directory of Open Access Journals (Sweden)

    Collado Antonia

    2006-05-01

    Full Text Available Abstract Background Phytopharmacological studies of different Calendula extracts have shown anti-inflamatory, anti-viral and anti-genotoxic properties of therapeutic interest. In this study, we evaluated the in vitro cytotoxic anti-tumor and immunomodulatory activities and in vivo anti-tumor effect of Laser Activated Calendula Extract (LACE, a novel extract of the plant Calendula Officinalis (Asteraceae. Methods An aqueous extract of Calendula Officinalis was obtained by a novel extraction method in order to measure its anti-tumor and immunomodulatory activities in vitro. Tumor cell lines derived from leukemias, melanomas, fibrosarcomas and cancers of breast, prostate, cervix, lung, pancreas and colorectal were used and tumor cell proliferation in vitro was measured by BrdU incorporation and viable cell count. Effect of LACE on human peripheral blood lymphocyte (PBL proliferation in vitro was also analyzed. Studies of cell cycle and apoptosis were performed in LACE-treated cells. In vivo anti-tumor activity was evaluated in nude mice bearing subcutaneously human Ando-2 melanoma cells. Results The LACE extract showed a potent in vitro inhibition of tumor cell proliferation when tested on a wide variety of human and murine tumor cell lines. The inhibition ranged from 70 to 100%. Mechanisms of inhibition were identified as cell cycle arrest in G0/G1 phase and Caspase-3-induced apoptosis. Interestingly, the same extract showed an opposite effect when tested on PBLs and NKL cell line, in which in vitro induction of proliferation and activation of these cells was observed. The intraperitoneal injection or oral administration of LACE extract in nude mice inhibits in vivo tumor growth of Ando-2 melanoma cells and prolongs the survival day of the mice. Conclusion These results indicate that LACE aqueous extract has two complementary activities in vitro with potential anti-tumor therapeutic effect: cytotoxic tumor cell activity and lymphocyte activation

  17. INFLUENCIA DE LA FECHA DE SIEMBRA Y DE LA PROCEDENCIA DE LA SEMILLA EN EL RENDIMIENTO DE CAPÍTULOS DE Calendula officinalis L., DURANTE DOS TEMPORADAS EN CHILLÁN Influence of sowing date and seed origin on the yield of capitula of Calendula officinalis L. during two growing seasons in Chillán

    OpenAIRE

    Marisol Berti D.; Rosemarie Wilckens E.; Felicitas Hevia H.; Alejandro Montecinos Ll.

    2003-01-01

    La caléndula (Calendula officinalis L.) es una planta cuyas flores secas se utilizan como cicatrizantes externos y se comercializan como capítulos o lígulas secos. Para poder determinar el potencial de rendimiento de caléndula se realizó un ensayo en Chillán, Chile. Se evaluó el efecto de cuatro fechas de siembra y dos cultivares de caléndula en dos temporadas de crecimiento del cultivo. Las fechas de siembre fueron el 4 de junio, 5 de agosto, 7 de septiembre y 14 de octubre de 1998, y se usa...

  18. Influência do substrato e do tamanho da célula de bandejas de poliestireno expandido no desenvolvimento de mudas e produção de calêndula (Calendula officinalis L.) Influence of substrate and cell size of expanded polystyrene tray on the development and production of marigold (Calendula officinalis L.) seedlings

    OpenAIRE

    C.K.R Barbosa; S.V. Valadares; F.P.G. Bonfim; I.C.G Honorio; Martins, E. R.

    2010-01-01

    A calêndula (Calendula officinalis L.) é uma planta medicinal anual de origem Mediterrânica que apresenta propriedades antiinflamatórias, antivirais, antigenotóxicas. A propagação desta planta normalmente é feita por sementes, sendo o uso de bandejas de poliestireno uma forma econômica de produção de mudas. Foram conduzidos dois experimentos com o objetivo de avaliar a interferência de dois tamanhos de célula em bandeja de poliestireno expandido (40 cm³ e 12 cm³) e de dois substratos (solo + ...

  19. Eficiência nutricional do Nitrogênio e produção de biomassa em Calendula officinalis L. (Asteraceae) em condições de casa de vegetação Nitrogen nutritional efficiency and biomass production in Calendula officinalis L. (Asteraceae) under greenhouse conditions

    OpenAIRE

    A.P. Serra; M.E Marchetti; M.C. Vieira; A.D. Robaina; C.O. Veronesi; J.M. Nascimento; Matos, F.; V.A. Conrad; H.S. Morais; F.C.N. Guimarães

    2013-01-01

    O objetivo deste trabalho foi avaliar o efeito da aplicação de diferentes doses de nitrogênio no solo e de épocas de coleta sobre a eficiência nutricional do nitrogênio e a produção de biomassa em Calendula officinalis L. (Asteraceae) em condições de casa de vegetação. O substrato utilizado foi a camada de 0 - 20 cm de Latossolo Vermelho Distroférrico. O delineamento experimental foi inteiramente casualizado, com quatro repetições. Os tratamentos foram constituídos de quatro doses de N (0, 21...

  20. Produção de biomassa de calendula officinalis l. adubada com fósforo e cama-de-frango Biomass yield of Calendula officinalis L. fertilized with phosporus and chicken manure

    Directory of Open Access Journals (Sweden)

    Valdenise Carbonari Barboza

    2009-04-01

    Full Text Available Objetivou-se, neste trabalho, avaliar a influência do fósforo e da cama-de-frango semidecomposta na produção de capítulos florais da Calendula officinalis L. Os fatores em estudo foram cinco doses de fósforo (4,3; 25,8; 43,0; 60,2; 81,7 kg ha-1 de P2O5, na forma de superfosfato triplo e cinco doses de cama-de-frango semidecomposta (1.000; 6.000; 10.000; 14.000 e 19.000 kg ha-1. Para combinar o fósforo e a cama-de-frango, usou-se a matriz experimental Plan Puebla III resultando em nove tratamentos (25,8 e 6.000; 60,2 e 6.000; 25,8 e 14.000; 60,2 e 14.000; 43,0 e 10.000; 4,3 e 6.000; 81,7 e 14.000; 25,8 e 1.000; 60,2 e 19.000 kg ha-1 de P e cama-de-frango, respectivamente no delineamento de blocos casualizados, com quatro repetições. A altura média final das plantas, aos 120 dias, ficou em torno de 60,0 cm, independente dos tratamentos. A produção máxima de capítulos florais da calêndula foi de 9.755,16 mil ha-1,obtida com o uso de 49,15 kg ha-1 de P2O5 associada a 13.401,10 kg ha-1 de cama-de-frango. A maior produção (11.058,89 kg ha-1de massa fresca de capítulos florais de calêndula foi obtida com 49,98 kg ha-1 de P2O5 e 13.266,59 kg ha-1 de cama-de-frango. As produções máximas de massas secas de capítulos florais (2.227,61 kg ha-1 foram obtidas com 39,62 kg ha-1 de P2O5 e 8.109,75 kg ha-1 de cama-de-frango. Concluiu-se que para se obter maior produção de massa seca de capítulos florais, componente de maior importância comercial, a calêndula deve ser cultivada utilizando adubação com HH 40 kg ha-1 de P2O5 e H" 8.000 kg ha-1 de cama-de-frango.The aim of this work was to evaluate the influence of phosphorus and semi-decomposed chicken manure on capitula yield of Calendula officinalis L. The studied factors were five doses of phosphorus (4.3, 25.8, 43.0, 60.2, and 81.7 kg ha-1 P2O5 in the triple super phosphate form and five doses of semi-decomposed chicken manure (1000, 6000, 10000, 14000, and 19000 kg ha-1. The Plan

  1. EFFECT OF POLYPHENOLIC COMPOUNDS ISOLATED FROM CARTHAMUS TINCTORIUS AND CALENDULA OFFICINALIS L., ON FUNCTIONAL ACTIVITY OF IMMUNE CELLS UNDER CONDITIONS OF CYTOSTATIC IMMUNOSUPPRESSION

    Directory of Open Access Journals (Sweden)

    N. V. Masnaya

    2013-01-01

    Full Text Available The purpose of the study – to study the effect of polyphenolic compounds extracted from the flowers of safflower oil and calendula, the functional activity of immune cells in cytotoxic immune suppression.Conventional methods determined the total number of splenocytes, relative (% and absolute (106, the number of antibody-forming cells (AFC in the spleen of mice by local hemolysis by Cunningham.Evaluated the effect of compounds of natural origin on the cellular immune response in the delayed-type hypersensitivity (DTH. Phagocytic activity of peritoneal macrophages was determined by the method based on the intensity of their capture ink particles. We studied the functional activity of peritoneal macrophages by NBT test (spontaneous and stimulated. Studies were conducted on male mice Category 1 (conventional linear mouse line CBA/CaLac aged 2–2.5 months, weighing 20–22 g. After the introduction mice line CBA/CaLac of polyphenolic compounds derived from flowers of Carthamus tinctorius and flowers of Calendula officinalis L. during the 5-day course in dose 50 mg/kg was observed stimulation of the humoral immune response (total number of splenocytes, the number of antibodies in the spleen cells and the functional activity of macrophages and Immunomodulating effect on the humoral immunity and the functional activity of macrophages after a single injection of cyclophosphamide in dose 250 mg/kg. Immunotropic activity of polyphenolic compounds is higher than that those of the reference product of tincture of Echinacea purpurea.

  2. Morphology, anatomy and influence of drying on germination of seeds of Calendula officinalis Morfoanatomía y efecto del secado en la germinación de semillas de caléndula y eneldo

    OpenAIRE

    Victoria T. Jorge A.; Sánchez O Manuel Salvador; Bonilla C Carmen Rosa

    2007-01-01

    Laboratory tests with seed of Calendula officinalis and Anethum graveolens to describe morphology and anatomy components such as humidity content in balance (CHB) in different atmospheres to get appropriate conservation of the germsplasm to determine physiological behavior in short seed storage to evaluate and to stablish an appropriate methodology for germination, were carried out. The morphological and anatomical description allowed to specify and to illustrate the organs and internal struc...

  3. Cicatrização de úlceras por pressão com extrato Plenusdermax® de Calendula officinalis L.

    Directory of Open Access Journals (Sweden)

    Marcelo Buzzi

    2016-04-01

    Full Text Available RESUMO Objetivo: Avaliar os benefícios terapêuticos do extrato de bioativos de Calendula officinalis Plenusdermax® na cicatrização de úlceras de pressão. Métodos: estudo observacional de coorte realizado com quarenta e um pacientes com diagnóstico de úlcera por pressão com tamanho da ferida estável por mais de três meses. Os pacientes foram avaliados quinzenalmente durante 30 semanas, em relação a redução da área da lesão, controle de infecção, tipos de tecido e exsudato e microbiologia das úlceras. Resultados: a proporção de pacientes que cicatrizaram completamente após 15 e 30 semanas de tratamento foi 63% e 88%, respectivamente, sendo que a média de tempo de cicatrização foi de 12,5 ± 7,8 semanas. Não foram observados eventos adversos durante o tratamento. Conclusão: os resultados do estudo indicam que Plenusdermax® de bioativos de C. officinalis é um tratamento seguro que promove a cicatrização de úlceras de pressão.

  4. Seed germination of calendula in response to temperature

    Science.gov (United States)

    Calendula (Calendula officinalis L.) has been used historically for herbal medicinal purposes and as an ornamental plant. With the discovery that calendula seeds contain high concentrations of calendic acid (C18:3) in the 1980s it began to be investigated as an oilseed crop for use in paint, coating...

  5. A review on phytochemistry and ethnopharmacological aspects of genus Calendula

    OpenAIRE

    Disha Arora; Anita Rani; Anupam Sharma

    2013-01-01

    This review includes 84 references on the genus Calendula (Asteraceae) and comprises ethnopharmacology, morphology and microscopy, phytoconstituents, pharmacological reports, clinical studies and toxicology of the prominent species of Calendula. Triterpene alcohols, triterpene saponins, flavonoids, carotenoids and polysaccharides constitute major classes of phytoconstituents of the genus. A few species of this genus have medicinal value, among these Calendula officinalis Linn., has been tradi...

  6. Calendula and camelina response to nitrogen fertility

    Science.gov (United States)

    The emerging oil-seed crops calendula (Calendula officinalis) and camelina (Camelina sativa L.) can provide a domestic, renewable, non-food alternative to imported oil sources for bioenergy and industrial purposes. However, very little information exists concerning nitrogen (N) fertilizer guidelines...

  7. Post-emergence herbicides useful in calendula

    Science.gov (United States)

    Easy and effective weed control is required by growers who are considering new industrial crops. Post-emergence herbicides typically are the products of choice by today’s growers. Unfortunately, post-emergence herbicides with proven safety margins are not known for calendula (Calendula officinalis),...

  8. Teor e rendimento de flavonóides em calêndula (Calendula officinalis L.) cultivada com diferentes lâminas de irrigação Flavonoid content and yield in marigold plant (Calendula officinalis L.) cultivated under different irrigation depths

    OpenAIRE

    D.P.G. Bortolo; P.A.A. Marques; A.C. PACHECO

    2009-01-01

    A Calendula officinalis L. apresenta uso ornamental, culinário e terapêutico. A inflorescência é usada com ação cicatrizante e antiinflamatória. O experimento analisou o efeito de quatro lâminas de irrigação em condições de campo, na produção de flavonóides em calêndula. As lâminas baseadas na evaporação do Tanque Classe A (ECA) foram: 0, 50, 100 e 150% ECA; sendo aplicadas desde o início do florescimento até o final do ciclo da cultura. O teor de flavonóides não apresentou diferenças estatís...

  9. Eficiência nutricional do Nitrogênio e produção de biomassa em Calendula officinalis L. (Asteraceae em condições de casa de vegetação Nitrogen nutritional efficiency and biomass production in Calendula officinalis L. (Asteraceae under greenhouse conditions

    Directory of Open Access Journals (Sweden)

    A.P. Serra

    2013-01-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito da aplicação de diferentes doses de nitrogênio no solo e de épocas de coleta sobre a eficiência nutricional do nitrogênio e a produção de biomassa em Calendula officinalis L. (Asteraceae em condições de casa de vegetação. O substrato utilizado foi a camada de 0 - 20 cm de Latossolo Vermelho Distroférrico. O delineamento experimental foi inteiramente casualizado, com quatro repetições. Os tratamentos foram constituídos de quatro doses de N (0, 21, 42 e 84mg dm-3 e cinco épocas de colheita (15, 30, 45, 60 e 90 dias após o transplante. O teor de N na inflorescência de calêndula reduziu com as épocas de coleta, redução essa que ocorreu possivelmente devido à menor eficiência da absorção desse elemento pela planta e menor translocação do N para as inflorescências. Pode se concluir que as épocas de avaliação influenciaram na resposta da planta à eficiência de absorção (EFA, sendo a máxima EFA de N aos 51 dias após o transplante das mudas. Observou-se aumento na translocação de N da parte aérea (folhas e caules para as inflorescências, tendo como consequência a redução do N na parte aérea. Houve incremento na produção de biomassa de calêndula em função das épocas de coleta , e do aumento das doses de N aplicadas no solo. A eficiência nutricional do N foi influenciada pelas épocas de avaliação e pelo teor de N presente no solo.The aim of this study was to evaluate the effect of both the application of different nitrogen levels on the soil and the harvest periods on nitrogen nutritional efficiency and biomass production in Calendula officinalis L. (Asteraceae under greenhouse conditions. The employed substrate was 0-20cm layer of Oxisol. Experimental design was completely randomized, with four replicates. Treatments consisted of four N levels (0, 21, 42 and 84 mg dm-3 and five harvest periods (15, 30, 45, 60 and 90 days after transplanting. N levels in

  10. Identificação dos constituintes químicos da fração volátil da Calendula officinalis produzida no Paraná Chemical compounds of the Calendula officinalis volatile fraction produced in the Paraná State, Brazil

    OpenAIRE

    Zilda Cristiani Gazim; Gilberto Alves Ferreira; Claudia Moraes Rezende; Celso Vataru Nakamura; Benedito Prado Dias Filho; Diógenes Aparicio Garcia Cortez

    2007-01-01

    A Calendula officinalis L., pertence à família Asteraceae, é originária da Europa e está aclimatada na região Sul do Brasil, onde vem crescendo o seu cultivo comercial para fins farmacêuticos e cosméticos. Suas inúmeras propriedades terapêuticas entre as quais antiinflamatória, cicatrizante e anti-séptica, justificam a grande procura desta planta pela população. O presente trabalho compara a composição química da fração volátil da cultura de calêndula aclimatada na região Noroeste do Paraná, ...

  11. MITOSIS ANA-THELOPHASE CHROMOSOMAL ABERRATIONS INDUCED BY UV IRRADIATION UNDER THE ANTIOXIDATIVE PROTECTION OF VITAMIN C, BY CALENDULA OFFICINALIS L.

    Directory of Open Access Journals (Sweden)

    Csilla Iuliana Bara

    2014-07-01

    Full Text Available due to the stratospheric ozone layer depletion, the researches focused in the last decades on the study of solarradiations reaching Earth surface. The target of the study was to establish the biological response of Calendula officinalisL. to UV irradiation, under the anti-oxidative protection of vitamin C, (which helps next to UV induced anti-oxidativeenzymes, in the protection against a large variety of products capable to induce free radicals formation.Our investigations were focused on detecting chromosomal aberrations which occurs during cells in division in meristemroot tips, under UV irradiation for 15 or 30 minutes, in the presence or absence of vitamin C, reported to the non-irradiated control samples. Regarding the mitotic index, it could be noticed an inhibition of cell division frequency underUV stress for all irradiated variants, not depending of presence or absence of vitamin C, positively correlated with theincrease of irradiation period. Maximal chromosomal aberrations frequency, were induced by UV radiations in the roottips of seedlings germinated in the absence of vitamin C, decreasing in the presence of vitamin C, due to the antioxidantprotective role of this. In the absence of UV irradiation, the chromosomal aberrations frequency was lower comparingwith irradiated variants, for all seedlings, even if germinated in the presence or absence of vitamin C. Betweenchromosomal aberrations were detected: bridges, expulsed and retardate chromosomes, fragments.

  12. A Prospective, Descriptive Study to Assess the Clinical Benefits of Using Calendula officinalis Hydroglycolic Extract for the Topical Treatment of Diabetic Foot Ulcers.

    Science.gov (United States)

    Buzzi, Marcelo; de Freitas, Franciele; Winter, Marcos

    2016-03-01

    Diabetic foot ulcers (DFUs) have a significant impact on patient quality of life. A prospective, descriptive pilot study was conducted between May 2012 and December 2013 through the dermatology outpatient unit in a Brazilian hospital to evaluate the clinical benefits of using Calendula officinalis hydroglycolic extract in the treatment of DFUs. Patients diagnosed with a stable neuropathic ulcer of >3 months' duration; ranging in size from 0.5-40 cm(2); without osteomyelitis, gangrene, bone exposure, cancer, or deep tissue infection; ages 18-90 years; with adequate glycemic control and no history of an allergy to C. officinalis were enrolled. Patients provided demographic and diabetes-related information and were evaluated biweekly for 30 weeks or until healing (ie, full epithelialization with no wound drainage). DFUs were measured and clinically examined for microbiological flora and presence of odor, tissue type (eg, granulation, fibrin sloth, necrosis), exudate, and retraction rate using planimetry images. Patients' blood tests and neuropathic pain assessment (the latter by clinician-directed questionnaire) were performed at baseline and the end of treatment; pain also was assessed during dressing changes using a 10-point rating scale. Patients' ulcers were treated twice daily with C. officinalis hydroglycolic extract spray solution and covered with saline-moistened, sterile, nonadherent gauze and bandages followed by foot offloading with adequate protective footwear. Patients received their first treatment in the clinic then performed care at home. From a potential population of 109 patients, 25 did not meet the inclusion criteria. Of the remaining 84 participants enrolled, 43 withdrew before study completion; cited reasons included lost to follow-up (16), medical judgment (2), failure to attend >3 scheduled visits (17), protocol violation (5), and death (3). Forty-one (41) - 17 women, average age 62 years (range 44-82 years), average glycemic level 153 mg

  13. A Prospective, Descriptive Study to Assess the Clinical Benefits of Using Calendula officinalis Hydroglycolic Extract for the Topical Treatment of Diabetic Foot Ulcers.

    Science.gov (United States)

    Buzzi, Marcelo; de Freitas, Franciele; Winter, Marcos

    2016-03-01

    Diabetic foot ulcers (DFUs) have a significant impact on patient quality of life. A prospective, descriptive pilot study was conducted between May 2012 and December 2013 through the dermatology outpatient unit in a Brazilian hospital to evaluate the clinical benefits of using Calendula officinalis hydroglycolic extract in the treatment of DFUs. Patients diagnosed with a stable neuropathic ulcer of >3 months' duration; ranging in size from 0.5-40 cm(2); without osteomyelitis, gangrene, bone exposure, cancer, or deep tissue infection; ages 18-90 years; with adequate glycemic control and no history of an allergy to C. officinalis were enrolled. Patients provided demographic and diabetes-related information and were evaluated biweekly for 30 weeks or until healing (ie, full epithelialization with no wound drainage). DFUs were measured and clinically examined for microbiological flora and presence of odor, tissue type (eg, granulation, fibrin sloth, necrosis), exudate, and retraction rate using planimetry images. Patients' blood tests and neuropathic pain assessment (the latter by clinician-directed questionnaire) were performed at baseline and the end of treatment; pain also was assessed during dressing changes using a 10-point rating scale. Patients' ulcers were treated twice daily with C. officinalis hydroglycolic extract spray solution and covered with saline-moistened, sterile, nonadherent gauze and bandages followed by foot offloading with adequate protective footwear. Patients received their first treatment in the clinic then performed care at home. From a potential population of 109 patients, 25 did not meet the inclusion criteria. Of the remaining 84 participants enrolled, 43 withdrew before study completion; cited reasons included lost to follow-up (16), medical judgment (2), failure to attend >3 scheduled visits (17), protocol violation (5), and death (3). Forty-one (41) - 17 women, average age 62 years (range 44-82 years), average glycemic level 153 mg

  14. Potencial de extratos à base de Calendula officinalis L. na indução da síntese de fitoalexinas e no efeito fungistático sobre Botrytis cinerea, in vitro Potential of Calendula officinalis L. extracts in inducing phytoalexin synthesis and fungistatic effect on Botrytis cinerea in vitro

    Directory of Open Access Journals (Sweden)

    S.M. Mazaro

    2013-01-01

    Full Text Available Foram desenvolvidos três experimentos com o objetivo de avaliar o potencial de preparados a base de calêndula (Calendula officinalis L. na indução de fitoalexinas em cotilédones de soja, na indução de mecanismos de resistência em frutos de morango, e o efeito fungistático sobre Botrytis cinerea in vitro. O delineamento experimental foi inteiramente casualizado com quatro repetições, para 15 tratamentos resultantes da combinação de três formas de extração (extrato alcoólico, infusão, e maceração em cinco concentrações (zero; 1,25; 2,5; 5; e 10%. Os resultados demonstram que os preparados de C. officinalis apresentaram capacidade de indução das fitoalexinas gliceolinas em cotilédones de soja. Na aplicação dos preparados em pós-colheita de morangos ocorreu alteração no teor de flavonóides, bem como a atividade da enzima FAL foi estimulada pela aplicação dos extratos; no entanto, não foi constatado o controle de podridão dos frutos. O efeito fungistático foi observado na extração por maceração em todas as suas concentrações reduzindo o crescimento do fungo B. cinerea in vitro sendo que, a partir de 2,5%, observou-se inibição total. A extração por infusão também apresentou resposta positiva na redução do crescimento de B. cinerea, com melhor resposta na concentração de 10% do preparado.Three experiments were carried out to evaluate the potential of calendula (Calendula officinalis L. extracts for phytoalexin induction in soybean cotyledons, resistance mechanism induction in strawberry fruits and fungistatic effect on Botrytis cinerea in vitro. Experimental design was completely randomized with 15 treatments resulting from the combination of three forms of extraction (alcohol extract, infusion and maceration at five concentrations (zero, 1.25, 2.5, 5 and 10%. Results showed that C. officinalis extracts could induce the phytoalexins glyceollins in soybean cotyledons. In the application of extracts

  15. Morfoanatomía y efecto del secado en la germinación de semillas de caléndula y eneldo Morphology, anatomy and influence of drying on germination of seeds of Calendula officinalis and Anethum graveolens

    OpenAIRE

    Jorge A Victoria T; Carmen R Bonilla C; Manuel S. Sánchez O

    2007-01-01

    En semillas de caléndula Calendula officinalis y de eneldo Anethum graveolens , se realizaron la descripción morfológica y anatómica y mediciones del contenido de humedad en equilibrio (CHE). La descripción morfológica y anatómica permitió precisar e ilustrar detalladamente los órganos y tejidos internos de la semilla, además, clarificar aspectos en la definición de semilla pura de caléndula. Se elaboraron curvas de secado y se midió el CHE en diferentes ambientes de secamiento artificial, ob...

  16. Avaliação da espalhabilidade e do teor de flavonóides em forma farmacêutica semi-sólida contendo soluções extrativas de Calendula officinalis L.

    Directory of Open Access Journals (Sweden)

    Júlio Cézar Borella

    2010-11-01

    Full Text Available

    O objetivo deste estudo foi avaliar característica reológica (espalhabilidade e quantificar os princípios ativos (flavonóides de preparações semi-sólidas (pomadas contendo extratos de Calendula officinalis. A partir da droga vegetal desta espécie foram obtidas as soluções extrativas preparadas por maceração e posterior percolação, na proporção de 1:5, utilizando-se mistura hidroalcóolica (etanol:água 62:38 e hidroglicólica (propilenoglicol:água 9:1. As pomadas contendo estes extratos e como excipientes lanolina e vaselina foram preparadas por duas técnicas distintas: por solução (a quente e por suspensão (a frio. Os resultados demonstraram que as pomadas contendo extratos hidroalcoólicos e hidroglicólicos, preparadas por suspensão (a frio exibem as maiores relações entre área e o esforço limite (0,34 e 0,33 mm2 g-1, respectivamente, no entanto as pomadas preparadas com extratos hidroalcoólicos por suspensão resultaram nos maiores valores de flavonóides totais (0,04% m/m calculados como hiperosídeos. Palavras-chave: Calendula officinalis. Pomada. Reologia. Flavonoides. fitoterápicos. ABSTRACT The aim of this study was to assess the rheological properties (spreadability and assay the active ingredients (flavonoids in semi-solid preparations (ointments containing extracts of marigold (Calendula officinalis L. - Asteraceae. The herbal drug from this species was separated from the dried inflorescence and two types of extracts were prepared by percolation at a ratio of 1:5, one with an ethanol:water mixture (62:38 and the other with propylene glycol: water (9:1. Ointments were prepared from these extracts, lanolin and vaseline, by two different techniques: solution (hot and suspension (cold. Samples of each ointment were analyzed, to determine the spreadability, as the ratio between area of spread and limiting applied weight, and the flavonoid content. The results showed that ointments prepared by

  17. 金盏花药学研究概况%Overview of Pharmacological Research on Calendula officinalis L.

    Institute of Scientific and Technical Information of China (English)

    孙曼; 赵兵; 姚默; 李祥楠; 廖雁; 巩江; 倪士峰

    2011-01-01

    Based on extensive literature search, this paper summarized species, composition, pharmacology, clinical application of C. Officinalis , to provide scientific materials for further development and utilization.%在广泛文献检索基础上,对金盏花的种属、成分、药理、临床应用等进行了概述,为其深入开发利用提供了科学资料.

  18. Teor e rendimento de flavonóides em calêndula (Calendula officinalis L. cultivada com diferentes lâminas de irrigação Flavonoid content and yield in marigold plant (Calendula officinalis L. cultivated under different irrigation depths

    Directory of Open Access Journals (Sweden)

    D.P.G. Bortolo

    2009-01-01

    Full Text Available A Calendula officinalis L. apresenta uso ornamental, culinário e terapêutico. A inflorescência é usada com ação cicatrizante e antiinflamatória. O experimento analisou o efeito de quatro lâminas de irrigação em condições de campo, na produção de flavonóides em calêndula. As lâminas baseadas na evaporação do Tanque Classe A (ECA foram: 0, 50, 100 e 150% ECA; sendo aplicadas desde o início do florescimento até o final do ciclo da cultura. O teor de flavonóides não apresentou diferenças estatísticas. Os maiores valores de matéria seca de flores e rendimento de flavonóides foram obtidos no tratamento sem irrigação. O teor de prolina foi maior para o tratamento com estresse por excesso hídrico. A deficiência hídrica acumulada para o tratamento sem irrigação sinalizou uma possível morte por deficiência hídrica. Com a ocorrência da precipitação no período de pré-florescimento, a planta, originária do Mediterrâneo, respondeu com florescimento abundante em massa e em número de flores. Assim, para Presidente Prudente, caso não ocorra a reposição hídrica do solo por precipitação, é necessário o uso de irrigação complementar com 200 mm apenas no período de pré-florescimento, para induzir ao máximo florescimento e máximo rendimento de flavonóides por hectare.Calendula officinalis L. presents ornamental, culinary and therapeutic use. Its inflorescence is used to heal and as an anti-inflammatory. This study analyzed the effect of four different irrigation depths under field conditions on flavonoid production in marigold plant (Calendula officinalis L.. The water depths were estimated based on the class A pan evaporation (CPE: 0, 50, 100 and 150% CPE; they were applied from the beginning of flowering to the end of the culture cycle. There were no statistical differences in flavonoid content. The highest values of flower dry weight and flavonoid yield were obtained with the treatment without irrigation

  19. 卢旺达产金盏菊花化学成分研究Ⅱ%Study on the constituents from Rwandan Calendula officinalis flos Ⅱ

    Institute of Scientific and Technical Information of China (English)

    陈玥; 李晓霞; 武一玫; 董勇喆; 王涛; 张祎

    2014-01-01

    目的 对卢旺达产金盏菊(Calendula officinalisL)的化学成分进行研究,为进一步开发利用该植物资源提供依据.方法 采用大孔吸附树脂、葡聚糖凝胶Sephadex LH-20等柱色谱及制备型高效液相色谱等方法进行分离纯化,并通过理化性质与波谱学数据相结合的方法鉴定化合物的结构.结果 从金盏菊中分离鉴定了6个单体成分,分别为金盏菊苷A(1,calendnoside A)、异鼠李素(2,isorhamnetin)、异鼠李素3-O-β-D-吡喃葡萄糖苷(3,isorhamnetin 3-O-β-D-glucopyranoside)、异鼠李素3-O-(6"-乙酰基)-β-D-吡喃葡萄糖苷(4,isorhamnetin 3-O-(6"-acetyl)-β-D-glucopyranoside)、异鼠李素3-O-β-D-呋喃洋芹糖基(1→2)-β-D-吡喃葡萄糖苷(5,isorhamnetin 3-O-β-D-apiofurranosyl(1→2)-β-D-glucopyranoside)和isorhamnetin 3-O-(2-O-β-xylopyranosyl-6-O-β-rhamnopyranosyl)-β-glucopyranoside (6).结论 化合物1为新化合物,化合物4~6为首次从金盏菊属植物中分离得到.

  20. Effects of nitrogen application and plant densities on flower yield, essential oils, and radiation use efficiency of Marigold (Calendula officinalis L.)

    International Nuclear Information System (INIS)

    Efficient use of radiation for medicinal plants production, might increase flower yield, essential oils and extract yield .A split plot design.was used in a two years (2005 and 2006) field study in Torogh region(36,10° N,59.33° E and 1300 m altitude) of Mashhad, Iran, to observe the effects of different nitrogen application and plants densities on flower dry matter production, essential oils, and radiation use efficiency in a multi-harvested Marigold (Calendula officinalis). The levels of nitrogen fertilizer were 0, 50, 100 and 150 kg ha-1 and levels of density were 20, 40, 60 and 80 plant m-2. The combined analysis results revealed significant effects of nitrogen and density levels on flower dry matter production, essential oils, and radiation use efficiency of Marigold. The highest dry flower production obtained by 150 kg ha-1 N and 80 plant m-2 plant population (102.86 g m-2). The higher flower dry matter production caused more essential oils and extract production in high nitrogen and density levels. The amount of essential oils and extract per 100g flower dry matter decreased during the flower harvesting period. The higher amount of essential oil and extract obtained at early flowering season. The essential oil and extract ranged from 0.22 to 0.12 (ml. per 100g flower dry matter) and 2.74 to 2.13 (g per 100g flower dry matter) respectively. Increase of both nitrogen and density caused higher radiation use efficiency. The most radiation use efficiency obtained at 150 kg ha-1 nitrogen and 80 Plant m-2desity treatments. In 150 kg ha-1 nitrogen treatment, increase of density levels from 20 plant m-2 to 80 Plant m-2 caused increase in radiation use efficiency from 1.41 g MJ-1 to 1.44 g MJ-1 respectively

  1. Repellency Effects of Essential Oils of Myrtle (Myrtus communis, Marigold (Calendula officinalis Compared with DEET against Anopheles stephensi on Human Volunteers

    Directory of Open Access Journals (Sweden)

    M Tavassoli

    2011-12-01

    Full Text Available Background: Malaria and leishmaniasis are two most significant parasitic diseases which are endemic in Iran. Over the past decades, interest in botanical repellents has increased as a result of safety to human. The comparative effi­cacy of essential oils of two native plants, myrtle (Myrtus communis and marigold (Calendula officinalis collected from natural habitats at southern Iran was compared with DEET as synthetic repellent against Anopheles stephensi on human subjects under laboratory condition. Methods:  Essential oils from two species of native plants were obtained by Clevenger-type water distillation. The protec­tion time of DEET, marigold and myrtle was assessed on human subject using screened cage method against An. stephensi. The effective dose of 50% essential oils of two latter species and DEET were determined by modified ASTM method. ED50 and ED90 values and related statistical parameters were calculated by probit analysis.   Results: The protection time of 50% essential oils of marigold and myrtle were respectively 2.15 and 4.36 hours com­pared to 6.23 hours for DEET 25%. The median effective dose (ED50 of 50% essential oils was 0.1105 and 0.6034 mg/cm2 respectively in myrtle and marigold. The figure for DEET was 0.0023 mg/cm2. Conclusion: This study exhibited that the repellency of both botanical repellents was generally lower than DEET as a synthetic repellent. However the 50% essential oil of myrtle showed a moderate repellency effects compared to mari­gold against An. stephensi.

  2. Repellency Effects of Essential Oils of Myrtle (Myrtus Communis, Marigold (Calendula Officinalis Compared with DEET Against Anopheles Stephensi on Human Volunteers

    Directory of Open Access Journals (Sweden)

    M Khoobdel

    2011-12-01

    Full Text Available Background: Malaria and leishmaniasis are two most significant parasitic diseases which are endemic in Iran. Over the past decades, interest in botanical repellents has increased as a result of safety to human. The comparative effi­cacy of essential oils of two native plants, myrtle (Myrtus communis and marigold (Calendula officinalis collected from natural habitats at southern Iran was compared with DEET as synthetic repellent against Anopheles stephensi on human subjects under laboratory condition. Methods: Essential oils from two species of native plants were obtained by Clevenger-type water distillation. The protec­tion time of DEET, marigold and myrtle was assessed on human subject using screened cage method against An. stephensi. The effective dose of 50% essential oils of two latter species and DEET were determined by modified ASTM method. ED50 and ED90 values and related statistical parameters were calculated by probit analysis. Results: The protection time of 50% essential oils of marigold and myrtle were respectively 2.15 and 4.36 hours com­pared to 6.23 hours for DEET 25%. The median effective dose (ED50 of 50% essential oils was 0.1105 and 0.6034 mg/cm2 respectively in myrtle and marigold. The figure for DEET was 0.0023 mg/cm2.Conclusion: This study exhibited that the repellency of both botanical repellents was generally lower than DEET as a synthetic repellent. However the 50% essential oil of myrtle showed a moderate repellency effects compared to mari­gold against An. stephensi.

  3. Calendula: A Potential New Oilseed Crop for the Northern U.S.

    Science.gov (United States)

    Seeds of calendula (Calendula officinalis) are a rich source of calendic acid (conjugated C18:3), a highly oxidative fatty acid that can be used to replace volatile organic compounds (VOC) as a drying agent in many industrial chemicals including paints and adhesives. Calendula flourishes in temperat...

  4. Influência do substrato e do tamanho da célula de bandejas de poliestireno expandido no desenvolvimento de mudas e produção de calêndula (Calendula officinalis L. Influence of substrate and cell size of expanded polystyrene tray on the development and production of marigold (Calendula officinalis L. seedlings

    Directory of Open Access Journals (Sweden)

    C.K.R Barbosa

    2010-03-01

    Full Text Available A calêndula (Calendula officinalis L. é uma planta medicinal anual de origem Mediterrânica que apresenta propriedades antiinflamatórias, antivirais, antigenotóxicas. A propagação desta planta normalmente é feita por sementes, sendo o uso de bandejas de poliestireno uma forma econômica de produção de mudas. Foram conduzidos dois experimentos com o objetivo de avaliar a interferência de dois tamanhos de célula em bandeja de poliestireno expandido (40 cm³ e 12 cm³ e de dois substratos (solo + esterco bovino curtido e substrato comercial tipo Plantmax para hortaliças® sobre a qualidade de mudas e a produção de calêndula. O delineamento estatístico nos dois experimentos foi em blocos casualizados, dispostos em arranjo fatorial 2 x 2 (dois substratos e dois tamanhos de células com seis repetições. Os tratamentos, referentes aos tamanhos das células foram bandeja com 128 células (40 cm³ de volume e, bandeja com 288 células (12 cm³ de volume. Os substratos utilizados foram solo de cerrado + esterco bovino curtido (1:1 e substrato comercial tipo "Plantmax para hortaliças®". Parte das mudas foi transplantada para avaliação da produção de capítulos. Houve diferenças significativas entre os substratos para as variáveis: matéria seca de parte aérea, matéria seca da raiz e matéria seca total. O uso do substrato formulado com solo de cerrado e esterco bovino proporcionou o melhor desenvolvimento das mudas de calêndula. Contudo, os diferentes tipos de substrato e bandejas de poliestireno não influenciaram significativamente a produção de capítulos das mudas transplantadas.Marigold Calendula (Calendula officinalis L. is an annual medicinal plant of Mediterranean origin which presents anti-inflammatory, antiviral and antigenotoxic properties. This species normally propagates through seeds, and the use of polystyrene trays is an economic way to produce its seedlings. Two experiments were carried out to evaluate the

  5. Controlled release of an extract of Calendula officinalis flowers from a system based on the incorporation of gelatin-collagen microparticles into collagen I scaffolds: design and in vitro performance.

    Science.gov (United States)

    Jiménez, Ronald A; Millán, Diana; Suesca, Edward; Sosnik, Alejandro; Fontanilla, Marta R

    2015-06-01

    Aiming to develop biological skin dresses with improved performance in the treatment of skin wounds, acellular collagen I scaffolds were modified with polymeric microparticles and the subsequent loading of a hydroglycolic extract of Calendula officinalis flowers. Microparticles made of gelatin-collagen were produced by a water-in-oil emulsion/cross-linking method. Thereafter, these microparticles were mixed with collagen suspensions at three increasing concentrations and the resulting mixtures lyophilized to make microparticle-loaded porous collagen scaffolds. Resistance to enzymatic degradation, ability to associate with the C. officinalis extract, and the extract release profile of the three gelatin-collagen microparticle-scaffold prototypes were assessed in vitro and compared to collagen scaffolds without microparticles used as control. Data indicated that the incorporation of gelatin-collagen microparticles increased the resistance of the scaffolds to in vitro enzymatic degradation, as well as their association with the C. officinalis flower extract. In addition, a sharp decrease in cytotoxicity, as well as more prolonged release of the extract, was attained. Overall results support the potential of these systems to develop innovative dermal substitutes with improved features. Furthermore, the gelatin-collagen mixture represents a low-cost and scalable alternative with high clinical transferability, especially appealing in developing countries. PMID:25787728

  6. Preclinical evaluation of collagen type I scaffolds, including gelatin-collagen microparticles and loaded with a hydroglycolic Calendula officinalis extract in a lagomorph model of full-thickness skin wound.

    Science.gov (United States)

    Millán, D; Jiménez, R A; Nieto, L E; Linero, I; Laverde, M; Fontanilla, M R

    2016-02-01

    Previously, we have developed collagen type I scaffolds including microparticles of gelatin-collagen type I (SGC) that are able to control the release of a hydroglycolic extract of the Calendula officinalis flower. The main goal of the present work was to carry out the preclinical evaluation of SGC alone or loaded with the C. officinalis extract (SGC-E) in a lagomorph model of full-thickness skin wound. A total of 39 rabbits were distributed in three groups, of 13 animals each. The first group was used to compare wound healing by secondary intention (control) with wound healing observed when wounds were grafted with SGC alone. Comparison of control wounds with wounds grafted with SGC-E was performed in the second group, and comparison of wounds grafted with SGC with wounds grafted with SGC-E was performed in the third group. Clinical follow-ups were carried in all animals after surgery, and histological and histomorphometric analyses were performed on tissues taken from the healed area and healthy surrounding tissue. Histological and histomorphometric results indicate that grafting of SGC alone favors wound healing and brings a better clinical outcome than grafting SGC-E. In vitro collagenase digestion data suggested that the association of the C. officinalis extract to SGC increased the SGC-E cross-linking, making it difficult to degrade and affecting its biocompatibility. PMID:26597789

  7. Physical chemistry evaluation of stability, spreadability, in vitro antioxidant, and photo-protective capacities of topical formulations containing Calendula officinalis L. leaf extract

    OpenAIRE

    Viviane Cecília Kessler Nunes Deuschle; Regis Augusto Norbert Deuschle; Mariana Rocha Bortoluzzi; Margareth Linde Athayde

    2015-01-01

    Calendula is used widely in cosmetic formulations that present phenolic compounds in their chemical constitution. The objective of our research was to develop and evaluate the stability of topical formulations containing 5% hydro-ethanolic extract of calendula leaves, including spreadability, and in vitro photo-protective, and antioxidant capacity. To evaluate the stability, we used organoleptic characteristics, pH, and viscosity parameters. Antioxidant capacity was measured by the DPPH (2,2-...

  8. Avaliação da espalhabilidade e do teor de flavonóides em forma farmacêutica semi-sólida contendo soluções extrativas de Calendula officinalis L.

    OpenAIRE

    JúLIO CéZAR BORELLA

    2010-01-01

    O objetivo deste estudo foi avaliar característica reológica (espalhabilidade) e quantificar os princípios ativos (flavonóides) de preparações semi-sólidas (pomadas) contendo extratos de Calendula officinalis. A partir da droga vegetal desta espécie foram obtidas as soluções extrativas preparadas por maceração e posterior percolação, na proporção de 1:5, utilizando-se mistura hidroalcóolica (etanol:água 62:38) e hidroglicólica (propilenoglicol:água 9:1). As pomadas co...

  9. Influência da adubação e da cobertura morta na produtividade e no teor de flavonóides de Calendula officinalis L. (Asteraceae Influence of fertilization and mulching on the productivity and flavonoid content of Calendula officinalis L. (Asteraceae

    Directory of Open Access Journals (Sweden)

    J.C Borella

    2011-01-01

    Full Text Available Desenvolveu-se ensaio de cultivo de Calendula officinalis, variando-se os tipos de adubação e utilização de cobertura morta na observação da produção de inflorescências, droga vegetal e quantificação dos flavonóides presentes nestes materiais.O delineamento experimental adotado foi de blocos casualizados com 3 repetições, em arranjo fatorial de 3 x 2, constituído de seis tratamentos, sendo 1. adubação química (100 kg de N ha-1 + 50 kg de P ha-1; 2. adubação química + cobertura morta (grama seca - Paspalum notatum; 3. adubação orgânica (esterco bovino curtido 50 t ha-1; 4. adubação orgânica + cobertura morta; 5. sem adubação química ou orgânica + cobertura morta; 6. sem adubação química ou orgânica e sem cobertura morta (testemunha. Os resultados de número capítulos produzidos, massa seca das inflorescências e massa da droga vegetal foram totalizados após cinco meses do plantio. A quantificação de flavonóides foi realizada através de método espectrofotométrico. Os dados foram analisados estatisticamente (ANOVA/Teste Tukey-Kramer-5% e mostraram diferenças significativas (70% superior no número de inflorescências, massa seca das inflorescências e massa da droga vegetal entre o tratamento com adubação orgânica (3 e os tratamentos sem adubação química ou orgânica com cobertura morta (5 e o testemunha (6. As demais comparações, inclusive o teor de flavonóides, entre os diversos tratamentos não resultaram em diferenças significativas.Calendula officinalis was experimentally grown under field conditions with different fertilization types and mulching in order to evaluate inflorescence production, plant drugs and flavonoid levels. Experimental design was in randomized blocks, with 3 replicates, in a 3x2 factorial arrangement, consisting of six treatments, as follows: 1. chemical fertilization (100 kg N ha-1 + 50 kg P ha-1; 2. chemical fertilization + mulch (dry grass - Paspalum notatum; 3

  10. Influence of Foliage Fertilizer Application on the Growth and Development of Calendula officinalis%叶面喷肥对金盏菊生长发育的影响

    Institute of Scientific and Technical Information of China (English)

    谭家伟

    2011-01-01

    Experiments of foliage fertilizer application were done with different concentrations of KH2PO4. The results showed that 2‰ KH2PO4 can not only promote the growth of the seedlings, but also advance the flowering period, increase the yield and quality of the flowers and chlorophyll content, satisfying the long-term nutritional needs of Calendula officinalis for its growth. The phosphorus and potassium fertilizer is suitable for the growth and development of potted plants of Calendula offwinalis.%选用不同浓度的KH2PO4分别对盆栽金盏菊进行了叶面喷肥试验。结果表明:2‰KH2PO4不仅可促进金盏菊幼苗生长,还可提旱花期,提高花的产量及品质,增加叶绿素含量,能满足金盏菊生长对养分的长期需求,即磷钾肥更适于盆栽金盏菊的生长发育。

  11. Variation in seed lipids in Calendula germplasm

    Science.gov (United States)

    Calendula officinalis (pot marigold) has considerable promise as an industrial crop, with a long history as an ornamental and medicinal plant. It is also marketed as an ingredient in cosmetics and a colorant. It produces unusual seed lipids, which can provide an additional market for commercial Ca...

  12. Physical chemistry evaluation of stability, spreadability, in vitro antioxidant, and photo-protective capacities of topical formulations containing Calendula officinalis L. leaf extract

    Directory of Open Access Journals (Sweden)

    Viviane Cecília Kessler Nunes Deuschle

    2015-03-01

    Full Text Available Calendula is used widely in cosmetic formulations that present phenolic compounds in their chemical constitution. The objective of our research was to develop and evaluate the stability of topical formulations containing 5% hydro-ethanolic extract of calendula leaves, including spreadability, and in vitro photo-protective, and antioxidant capacity. To evaluate the stability, we used organoleptic characteristics, pH, and viscosity parameters. Antioxidant capacity was measured by the DPPH (2,2-diphenyl-1-picrylhydrazyl method, and the photo-protective capacity by SPF spectrophotometric measure. All formulations were stable. The calendula extract formulations in gel and cream showed no significant variations in pH, and the cream formulations presented lower viscosity variations than gel formulations. The spreadability of the gel formulations was superior to those in cream. The formulations also presented good antioxidant capacities and an FPS of around 1.75. In accordance with the results, the formulations can be used as antioxidants, but considering the low SPF obtained, calendula cannot be considered as a stand-alone sunscreen, yet may well be tested in future studies towards verifying enhancement of synthetic sunscreens.

  13. ESSENTIAL OIL OF CALENDULA OFFICINALIS

    Directory of Open Access Journals (Sweden)

    Aziza Juraeva

    2014-11-01

    Full Text Available The article presents the results of research into component composition of raw calendula’s essential oil. The study was conducted by gas chromatography mass spectrophotometry method under the following conditions: column - CP-Wax58FFAPCB24,5 mmx 250mm x0,20 mmnominal, the mobile-phase-He (helium, speed-column mobile phase-1.0 ml /min, volume sample injection-5mkl; resolutions(5:1, in the Split, high-boiling chamber -220 ◦C, detector temperature 275◦C; Aux-200 ◦ C, column temperature 70C◦ gradually increased from 10◦C / min to 250◦C, maintained to the end of analysis temperature regime(250◦Cfor 5min.In the course of the research the following substances were indentified - 1H cyclopropazulen-0, 981%, α-amorphous-1, 926%, γ-cadinene-11, 598%, 1H-cyclopropazulen 4-oldecahydro-1-0.825% viridiflorol-2, 029%, β-cubeb -3.809%, T-1 muurololnaftalenol-8.522%.In addition, it was ascertained that in different parts of the world calendula’s essential oils differ from each other in qualitative composition and quantitative components.

  14. Effect of intercropping carrot (Daucus carota L. with french marigold (Tagetes patula nana L. and pot marigold (Calendula officinalis L. on the occurrence of some pests and quality of carrot yield

    Directory of Open Access Journals (Sweden)

    Beata Jankowska

    2012-12-01

    Full Text Available Intercropping combines different aspects of the interaction between organisms in ecosystems and may be classified as a pro-ecological method of plant cultivation limiting the harmful human interference in the environment, especially the use of chemicals. It also allows high yield, good quality, and economic productivity to be achieved. The aim of the present study, conducted in the years 2003–2004 in Mydlniki near Kraków, was to determine the effect of intercropping carrot Nardin F1 with French marigold (Tagetes patula nana L. 'Kolombina' and pot marigold (Calendula officinalis L. 'Promyk' on carrot yield, its quality, and the occurrence of pests. On average in both years of the study, intercropping did not reduce total or marketable carrot yield. In 2003 intercropping contributed to a significant increase in marketable yield compared with yield from sole cropping. In 2004 the dry matter content was higher in the roots of carrots grown with pot marigold. The treatments intercropped with both French and pot marigold were characterized by an increased content of sugars in carrot roots and in 2004 year – of carotenoids. Intercropping had a significant effect on the number of roots damaged by the carrot rust fly, Psila rosae, (the best in this respect was the combination with Tagetes and by nematodes (the best in this respect was the combination with Calendula. The number of larvae of carrot psyllid, Trioza viridula, was significantly lower in the plots where carrot was intercropped. The study did not find intercropping to have a positive effect on reducing the occurrence of the root aphid.

  15. Mastering analytical challenges for the characterization of pentacyclic triterpene mono- and diesters of Calendula officinalis flowers by non-aqueous C30 HPLC and hyphenation with APCI-QTOF-MS.

    Science.gov (United States)

    Nicolaus, Christoph; Sievers-Engler, Adrian; Murillo, Renato; D'Ambrosio, Michele; Lämmerhofer, Michael; Merfort, Irmgard

    2016-01-25

    Pentacyclic triterpene mono- and diesters have been isolated from Calendula officinalis flowers. GC-MS, APCI-Exactive Orbitrap HR-MS and NMR allowed to identify the triterpene skeleton in various samples (different triterpene mixtures from Calendula n-hexane extract). NMR provided evidence that triterpene diesters are present in the samples as well. However, the corresponding quasi-molecular ions could not be detected by APCI-Exactive Orbitrap HR-MS. Instability of triterpene diesters and loss of a fatty acid residue, respectively, in the ion-source made their MS detection challenging. Thus, a set of new APCI-QTOF-MS methods (using the TripleTOF 5600+ mass spectrometer) were developed which made it eventually possible to solve this problem and confirm the diester structures by MS via quasi-molecular ion [M+H](+) detection. Direct infusion APCI-QTOF MS experiments in MS/MS high sensitivity scan mode with low collision energy and multi-channel averaging acquisition (MCA) allowed the detection of quasi-molecular ions of triterpene diesters for the first time and unequivocally confirmed the presence of faradiol 3,16-dimyristate and -dipalmitate, as well as the corresponding mixed diesters faradiol 3-myristate,16-palmitate and faradiol 3-palmitate,16-myristate. Preferential loss of the fatty acid in 16-position made it possible to distinguish the mixed diesters by MS/MS spectra. Their chromatographic separations turned out to be challenging due to their bulkiness and extended molecular dimensions. However, separation could be achieved by an uncommon non-aqueous RPLC mode with an in-house synthesized C30 phase. Finally, two (U)HPLC-APCI-QTOF-MS methods with C18- and C30-based non-aqueous RPLC provided suitable, sensitive assays to monitor the presence of monoesters and diesters of various triterpenes (faradiol, maniladiol, arnidiol, arnitriol A and lupane-3β,16β,20-triol esters) in the n-hexane extract of C. officinalis with high mass resolution and good mass accuracy

  16. Avaliação do tratamento subcrônico com o extrato hidroalcoólico de Calendula officinalis L. sobre os parâmetros bioquímicos e hematológicos em ratas Wistar

    Directory of Open Access Journals (Sweden)

    E.J.R. Silva

    2005-06-01

    Full Text Available Os efeitos da administração oral subcrônica do extrato hidroalcoólico (EHA preparado de flores de Calendula officinalis L. foram investigados sobre os parâmetros hematológicos e bioquímicos em ratas Wistar adultas. Quarenta ratas (n=10/grupo foram tratadas durante 30 dias consecutivos com EHA por via oral nas doses de 0,25, 0,5, e 1,0 g/kg de peso e, em seguida, determinados os perfis bioquímico e hematológico e a massa dos órgãos. Os resultados mostram que durante o período do tratamento não se observou sinais de toxicidade ou morte. Os parâmetros bioquímicos e hematológicos, assim como a massa dos órgãos não foram modificados pela administração subcrônica do EHA, excetuando-se aumento significativo de 24,2% para uréia na maior dose estudada e aumento, respectivamente, de 62,3, 30,2 e 44,4%, para ALT. Na hematologia, registrou-se flutuação dentro dos valores de referência na contagem diferencial de neutrófilos, linfócitos e monócitos. Dessa forma, a administração subcrônica do extrato hidroalcoólico de Calendula officinalis não produz efeitos tóxicos sobre a maioria dos parâmetros bioquímicos e hematológicos estudados em ratas Wistar adultas. Entretanto, o aumento dos níveis séricos de uréia e alanina aminotransferase (ALT em doses elevadas sugere sobrecargas renal e hepática, respectivamente, as quais devem ser investigadas em maiores detalhes.

  17. EFFECTS OF HORMONES ADDITION FOR IN VITRO PLANT DEVELOPMENT OF CALENDULLA OFFICINALIS

    Directory of Open Access Journals (Sweden)

    IRINA-CLAUDIA ALEXA

    2015-05-01

    Full Text Available The current study presented in vitro plant development of Calendula officinalis on MS (Murashige and Skoog medium supplemented with different formula of hormones. The morphogenetic response was evaluated by recording the number of plantlets, the plant height and weight. The results showed that small amounts of hormones can improve the plantlets growth in the case of Calendula officinalis.

  18. EFFECTS OF HORMONES ADDITION FOR IN VITRO PLANT DEVELOPMENT OF CALENDULLA OFFICINALIS

    OpenAIRE

    IRINA-CLAUDIA ALEXA; PETRONEL PAVĂL; DANIELA NICUŢĂ; PETRONELA BRAN; OANA-IRINA PATRICIU; LUMINIŢA GROSU; ADRIANA LUMINITA FINARU

    2015-01-01

    The current study presented in vitro plant development of Calendula officinalis on MS (Murashige and Skoog) medium supplemented with different formula of hormones. The morphogenetic response was evaluated by recording the number of plantlets, the plant height and weight. The results showed that small amounts of hormones can improve the plantlets growth in the case of Calendula officinalis.

  19. Effect of Exogenous Matters on Development of Calendula officinalis under Seawater Stress%外源物影响海水胁迫金盏菊发育的效应

    Institute of Scientific and Technical Information of China (English)

    吴文杰; 王琼

    2010-01-01

    以金盏菊(Calendula officinalis)为材料,研究甜菜碱(Glycinebetaine,GB)和硫酸锌(ZnSO4)对遭受35%海水胁迫的金盏菊幼苗的形态建成及其生理生化特性的影响.结果表明:外源GB(100~300 mg/L)和ZnSO4(1×10-5mol/L)可以促进金盏菊种子的萌发和幼苗的形态建成,促进叶中叶绿素含量的积累,降低根系内丙二醛(MDA)含量,提高过氧化物酶(POD)的活性,增强植物的渗透调节能力,缓解海水盐分胁迫对金盏菊幼苗的伤害.

  20. 陇东黄土高原石油污染土壤环境因子对金盏菊(Calendula officinalis)-微生物联合修复的响应%Environmental factors responses to petroleum-contaminated soil in situ remediation by using a combination of Calendula officinalis and microbial agent in eastern Gansu Province

    Institute of Scientific and Technical Information of China (English)

    王金成; 井明博; 段春燕; 张希彪; 刘建新; 肖朝霞; 周天林

    2015-01-01

    为筛选适合陇东黄土高原石油污染土壤修复植物,选取当地“适生”植物金盏菊(Calendula officinalis)联合微生物菌剂开展了为期5个月的原位修复实验.采用常规方法和PCR-DGGE技术分析了修复过程中土壤理化性质、酶活性及土壤微生物群落遗传多样性等15项土壤环境因子的动态变化情况及其对金盏菊生态修复的响应.结果表明,随着原位修复的进行,①土壤脲酶、多酚氧化酶、过氧化氢酶及脱氢酶活性均不同程度呈增加的趋势(p<0.05).②土壤TPHs、pH、有机质、含盐率的变化情况不尽相同,但总体呈下降的趋势(p<0.05);土壤碱解氮、速效磷及速效钾含量总体呈上升趋势(p<0.05).③土壤微生物群落遗传多样性结果显示,Shannon-Wiener多样性指数和Pielou均匀度指数整体呈逐步增加的趋势.④PCA分析结果显示,土壤脲酶、脱氢酶及碱解氮等环境因子对金盏菊修复表现为促进作用,而有机质含量过高将不利于金盏菊的植物修复.金盏菊和微生物菌剂联合修复过程中,土壤多酚氧化酶、速效磷、脱氢酶及速效钾发挥主要作用,而土壤TPHs和含盐率过高则影响了联合修复效果.本研究旨在分析金盏菊用于陇东地区油污土壤的生态响应,结果有助于筛选出适合陇东黄土高原土壤类别-适生植物-土著降解菌群的组合,利用乡土物种的适生性降低修复成本,为陇东地区油污土壤生态修复提供新的技术方案和背景资料.

  1. A Review of the Use of Topical Calendula in the Prevention and Treatment of Radiotherapy-Induced Skin Reactions.

    Science.gov (United States)

    Kodiyan, Joyson; Amber, Kyle T

    2015-01-01

    Calendula is a topical agent derived from a plant of the marigold family Calendula Officinalis. Containing numerous polyphenolic antioxidants, calendula has been studied in both the laboratory and clinical setting for the use in treating and preventing radiation induced skin toxicity. Despite strong evidence in the laboratory supporting calendula's mechanism of action in preventing radiation induced skin toxicity, clinical studies have demonstrated mixed results. In light of the controversy surrounding the efficacy of calendula in treating and preventing radiodermatitis, the topic warrants further discussion. PMID:26783706

  2. The Effects of Leaf-Spraying Urea and Calcium Superphosphate on the Growth and Deveplpment of Calendula officinalis L.%叶面喷施尿素和过磷酸钙对金盏菊生长发育的影响

    Institute of Scientific and Technical Information of China (English)

    曾长立; 程辉; 张萍

    2004-01-01

    研究了在金盏菊(Calendula officinalis L.)的生长初期叶面喷施各种不同浓度配比的尿素和过磷酸钙对其营养性状和花发育的影响,结果表明:尿素和过磷酸钙配合进行叶面喷施,能促进生长,增加株高、叶片数及花朵数,其中以0.1%的尿素和1.0%的过磷酸钙配合效果最佳.

  3. A Review of the Use of Topical Calendula in the Prevention and Treatment of Radiotherapy-Induced Skin Reactions

    OpenAIRE

    Joyson Kodiyan; Amber, Kyle T.

    2015-01-01

    Calendula is a topical agent derived from a plant of the marigold family Calendula Officinalis. Containing numerous polyphenolic antioxidants, calendula has been studied in both the laboratory and clinical setting for the use in treating and preventing radiation induced skin toxicity. Despite strong evidence in the laboratory supporting calendula’s mechanism of action in preventing radiation induced skin toxicity, clinical studies have demonstrated mixed results. In light of the controversy s...

  4. A review on phytochemistry and ethnopharmacological aspects of genus Calendula

    Directory of Open Access Journals (Sweden)

    Disha Arora

    2013-01-01

    Full Text Available This review includes 84 references on the genus Calendula (Asteraceae and comprises ethnopharmacology, morphology and microscopy, phytoconstituents, pharmacological reports, clinical studies and toxicology of the prominent species of Calendula. Triterpene alcohols, triterpene saponins, flavonoids, carotenoids and polysaccharides constitute major classes of phytoconstituents of the genus. A few species of this genus have medicinal value, among these Calendula officinalis Linn., has been traditionally used in the treatment of various skin tumors, dermatological lesions, ulcers, swellings and nervous disorders as well as almost 200 cosmetic formulations, i.e., creams, lotions, shampoos. Despite a long tradition of use of some species, the genus has not been explored properly. In the concluding part, the future scope of Calendula species has been emphasized with a view to establish their multifarious biological activities and mode of action.

  5. A review on phytochemistry and ethnopharmacological aspects of genus Calendula.

    Science.gov (United States)

    Arora, Disha; Rani, Anita; Sharma, Anupam

    2013-07-01

    This review includes 84 references on the genus Calendula (Asteraceae) and comprises ethnopharmacology, morphology and microscopy, phytoconstituents, pharmacological reports, clinical studies and toxicology of the prominent species of Calendula. Triterpene alcohols, triterpene saponins, flavonoids, carotenoids and polysaccharides constitute major classes of phytoconstituents of the genus. A few species of this genus have medicinal value, among these Calendula officinalis Linn., has been traditionally used in the treatment of various skin tumors, dermatological lesions, ulcers, swellings and nervous disorders as well as almost 200 cosmetic formulations, i.e., creams, lotions, shampoos. Despite a long tradition of use of some species, the genus has not been explored properly. In the concluding part, the future scope of Calendula species has been emphasized with a view to establish their multifarious biological activities and mode of action. PMID:24347926

  6. A Review of the Use of Topical Calendula in the Prevention and Treatment of Radiotherapy-Induced Skin Reactions

    Directory of Open Access Journals (Sweden)

    Joyson Kodiyan

    2015-04-01

    Full Text Available Calendula is a topical agent derived from a plant of the marigold family Calendula Officinalis. Containing numerous polyphenolic antioxidants, calendula has been studied in both the laboratory and clinical setting for the use in treating and preventing radiation induced skin toxicity. Despite strong evidence in the laboratory supporting calendula’s mechanism of action in preventing radiation induced skin toxicity, clinical studies have demonstrated mixed results. In light of the controversy surrounding the efficacy of calendula in treating and preventing radiodermatitis, the topic warrants further discussion.

  7. Deficiência hídrica e aplicação de ABA nas trocas gasosas e no acúmulo de flavonoides em calêndula (Calendula officinalis L. = Water deficit and ABA application on leaf gas exchange and flavonoid content in marigold (Calendula officinalis L..

    Directory of Open Access Journals (Sweden)

    Ana Cláudia Pacheco

    2011-04-01

    Full Text Available O objetivo deste trabalho foi avaliar os efeitos da deficiencia hidrica e aplicacao de acido abscisico (ABA sobre aspectos fisiologicos e teor de flavonoides em plantas de calendula. Oexperimento foi instalado em condicoes de casa-de-vegetacao com plantas envasadas. No inicio do florescimento de plantas de calendula, foram aplicados quatro intervalos de suspensao da irrigacao (irrigacao diaria; tres; seis e nove dias sem irrigar, acompanhados por tres doses de ABA (0, 10 e 100 ƒÊM. Avaliou-se o conteudo relativo de agua na folha (CRA e as trocas gasosas, utilizando-se um analisador portatil por infravermelho (A: fotossintese liquida, gs: condutancia estomatica, E: transpiracao, Ci: concentracao intercelular de CO2 e EUA: eficiencia de uso daagua. Aos nove dias sem irrigacao ocorreram reducoes significativas em todas as variaveis de trocas gasosas analisadas, independente da aplicacao de ABA. Concluiu-se que o efeito principal do ABA foi o de causar diminuicao na gs, a qual foi acompanhada de reducao em A somente quando as plantas estavam desidratadas. As intensidades de deficiencia hidrica testadas nao causaram interferencia no acumulo de flavonoides nas inflorescencias. Entretanto, o ABA restringiu a biossintese de flavonoides, tanto nas plantas-controle como nas plantas submetidas a deficiencia hidrica.The goal of this study was to evaluate the effects of water deficit and abscisic acid (ABA application on physiological parameters and flavonoid production in marigold plant. The experiment was performed under nursery conditions with potted plants. It was tested water deficit by withholding water (control . diary irrigation, 3, 6 and9 days without irrigation followed by 3 ABA concentrations (0, 10 e 100 ƒÊM applied in the beginning of blooming. It was evaluated the relative water content and the leaf gas exchange using a portable infrared gas analyzer (A: net photosynthesis, gs: stomatal conductance, E: transpiration, Ci: CO2 intercellular

  8. 铅/镉复合污染物对金盏菊生长的影响及在其体内富集特征%The growth and bioaccumulation characteristic of Calendula officinalis L under lead/cadmium co-contamination

    Institute of Scientific and Technical Information of China (English)

    丁绍兰; 杜波; 范春辉

    2015-01-01

    Having a short grow th periodicity and a strong enrichment in biomass ,the adaption to adverse environment of ornamental plants is quite strong ,and the circulatory effect of food chain ,a potential threat to human health ,could be effectively avoided w hen using ornamental plants to restore contaminated soil with heavy metal ,so this technology are proved security and practicability .The pot experiments were conducted to study the effect of Pb/Cd com‐bined pollution on the grow th of Calendula officinalis L and the effect of soil moisture on the bioaccumulation behavior of the seedlings .The results indicated that the emergence rate remained 45% or more under the contamination condition ,the lead/cadmium in loess had a little effect on the biomass of Calendula officinalis L ,and the grow th process of seedlings could improve partly physical and chemical properties of contaminated loess .Compared with the CK ,the root morphology of seedlings was significantly changed by SEM so that Calendu‐la officinalis L can be applied to the combined stress of the heavy metals .Moreover ,it was unfavorable to the absorption and bioaccumulation of Pb/Cd if the soil moisture were too low or too high ,the root is the main organ for absorbing and accumulating cadmium ,and the ex‐traction ability of seedlings on cadmium was quite strong .%花卉类植物的抗逆环境能力较强,生长周期较短、生物量较大;利用花卉类植物修复重金属污染土壤,有效避免了食物链循环效应对人体健康的潜在威胁,因此,该修复技术的安全性较好、实用性较强。通过盆栽试验研究Pb/Cd复合污染对金盏菊(Calendula officinalis L )幼苗生长状况的影响,同时考察土壤持水量对Pb/Cd富集行为的影响。研究发现:污染胁迫条件下,金盏菊幼苗的出苗率都维持在45%以上;黄土Pb/Cd对金盏菊生物量的影响不大,金盏菊幼苗的生长过程能够一定程度上改良黄土

  9. Effects of Calendula Essential Oil-Based Cream on Biochemical Parameters of Skin of Albino Rats against Ultraviolet B Radiation

    OpenAIRE

    Mishra, Arun K.; Mishra, Amrita; Verma, Anurag; Chattopadhyay, Pronobesh

    2012-01-01

    Reactive oxygen species (ROS) generated from UV-B radiation have the capacity to cause oxidative decomposition which leads to the formation of toxic components as well as lipid peroxidation. Considering this fact, the present study was performed to evaluate the effect of a cream (O/W) containing the essential oil of Calendula officinalis on biochemical parameters of the skin of albino rats against UV-B radiation. The fingerprint analysis of Calendula essential oil was performed by HPLC with s...

  10. Metabolism of [3-3H]oleanolic acid in the isolated ''Calendula officinalis'' leaf cells and transport of the synthesized glycosides, to the cell wall and the extracellular space

    International Nuclear Information System (INIS)

    It has been shown for the first time that [3-3H]oleanolic acid glycosides formed in the cytosol of ''C. officinalis'' leaf cells are transported to the extracellular space in the form of pentaglucoside VI (44%), whereas glucuronides derived from [3-3H]oleanolic acid 3-O-monoglucuronide (29%) as well as a part of glucosides (24%) were transported into the cell walls. (author). 15 refs, 2 figs, 1 tab

  11. Metabolism of [3-{sup 3}H]oleanolic acid in the isolated ``Calendula officinalis`` leaf cells and transport of the synthesized glycosides, to the cell wall and the extracellular space

    Energy Technology Data Exchange (ETDEWEB)

    Szakiel, A.; Wasiukiewicz, I.; Janiszowska, W. [Warsaw Univ. (Poland). Katedra Biochemii

    1995-12-31

    It has been shown for the first time that [3-{sup 3}H]oleanolic acid glycosides formed in the cytosol of ``C. officinalis`` leaf cells are transported to the extracellular space in the form of pentaglucoside VI (44%), whereas glucuronides derived from [3-{sup 3}H]oleanolic acid 3-O-monoglucuronide (29%) as well as a part of glucosides (24%) were transported into the cell walls. (author). 15 refs, 2 figs, 1 tab.

  12. 施肥对金盏菊生长、开花及养分吸收的影响%Effects of Nitrogen,Phosphorus,and Potassium Fertilizer on the Growth,Blossom,and N,P,K Uptake in Calendula officinalis L.

    Institute of Scientific and Technical Information of China (English)

    张文君; 鲁剑巍; 蒋志平; 王亚艺

    2010-01-01

    研究了氮、磷、钾不同施肥处理对金盏菊(Calendula officinalis L.)生长、开花、干物质积累量及养分吸收的影响.结果表明:推荐施肥[N-P2O5-K2O:(0.4-0.2-0.3) g/kg]能有效促进金盏菊的生长及花朵开放,其中干物质积累量和总花朵数,分别比不施肥处理增加了264. 3%和323. 1%,比习惯施肥增加了16.0%和25.0%;不施肥、习惯施肥和推荐施肥三者干物质日积累量高峰均出现在移栽后29~35 d,分别为0. 15、 0. 43、 0.42 g/d;各处理氮、磷、钾吸收量之间均存在显著差异,推荐施肥处理N、P、K养分累积量分别为0.33、 0.04、 0.39 g/株,分别是不施肥处理的6.93倍、 3.94倍和3.61倍,为习惯施肥的1.49倍、 1.35倍和1.24倍,各处理金盏菊对N、P、K养分吸收比例变幅为1∶(0.13~0.24)∶(1.20~2.30),平均比例为1∶0. 17∶1. 65.

  13. АНТИОКСИДАНТНАЯ АКТИВНОСТЬ ЭКСТРАКТОВ CALENDULA OFFICINALIS L

    OpenAIRE

    Лубсандоржиева, Пунцык-нима

    2009-01-01

    Определены содержание биологически активных веществ и антиоксидантная активность экстрактов из цветков Calendula officinalis L. Экстракт из указанного сырья, содержащий липофильные и среднеполярные вещества, характеризуется большей антиоксидантной активностью, чем водный и 50% этанольный экстракты....

  14. 转 merA 基因金盏菊对环境中汞污染的修复效果%Remediation Effect of Transgenic Calendula officinalis with merA Gene on Mercury Pollution

    Institute of Scientific and Technical Information of China (English)

    张雷; 丁艳丽; 付涌玉

    2015-01-01

    The remediation effect of transgenic C.officinalis with merA gene obtained by Agrobacterium-mediated genetic transformation system on mercury pollution was analyzed to study the mercury enrichment rate.The results showed that the 84% Hg2+ in soil can be transported into leaves of transgenic C.officinalis with merA gene and then most Hg2+ in leaves can be converted into nontoxic Hg under the action of merA gene,which can increase its tolerance to HgCl2 stress.The proline and MDA content,and activity of SOD and POD of transgenic C.officinalis with merA gene are increased under Hg2+ stress to varying degrees.In conclusion,transgenic C.officinalis with merA gene has the higher tolerance to Hg2+ stress.%为了提高金盏菊植物对环境中重金属(Hg)污染的修复效果,构建 merA 重组表达载体质粒,再经农杆菌介导法建立遗传转化体系筛选出转 merA 基因的金盏菊植株,研究其对汞的富集率。结果表明:转 merA 基因金盏菊对土壤中 Hg2+的转移率提高84%,而且大部分 Hg2+在 merA 基因作用下,转化为无毒的 Hg 挥发到空气中,提高了其忍受 HgCl2的浓度;在 Hg2+胁迫下,转基因植株中脯氨酸含量、SOD 酶活性、POD 酶活性及 MDA 含量均有不同程度的提高。转基因金盏菊具有较强的抗重金属汞胁迫的能力。

  15. Effect of fertilisation and harvest period on polar metabolites of Calendula oficcinalis

    Directory of Open Access Journals (Sweden)

    Eduardo Felipe Alves Fernandes

    2013-10-01

    Full Text Available The present study evaluated the chemical profile of polar extracts of Calendula officinalis L., Asteraceae, that were grown under different cultivation conditions: chemical fertilisation, organic fertilisation and mulching. Furthermore, we investigated metabolite variations during plant development by comparing the metabolites from harvested plants at 60 and 120 days after planting. We used HPLC-DAD-MS/MS to tentatively identify metabolites. In total, we identified seven known compounds: five flavonoid glycosides and two caffeoylquinic acids derivatives. There were no statistically significant differences in the expression of metabolites from plants grown under the examined soil treatments. However, five substances varied according to harvest time, suggesting that the biosynthesis of polar metabolites of Calendula officinalis is not affected by changes in soil composition. Therefore, this plant could represent a source for phytomedicines with a constant content of polar metabolites.

  16. Effect of fertilisation and harvest period on polar metabolites of Calendula oficcinalis

    OpenAIRE

    Eduardo Felipe Alves Fernandes; Fernando Meloni; Júlio Cézar Borella; Norberto Peporine Lopes

    2013-01-01

    The present study evaluated the chemical profile of polar extracts of Calendula officinalis L., Asteraceae, that were grown under different cultivation conditions: chemical fertilisation, organic fertilisation and mulching. Furthermore, we investigated metabolite variations during plant development by comparing the metabolites from harvested plants at 60 and 120 days after planting. We used HPLC-DAD-MS/MS to tentatively identify metabolites. In total, we identified seven known compounds: five...

  17. Variability of decorative traits, response to the Aphis fabae attack and RAPD diversity in different genotypes of Calendula

    OpenAIRE

    Adriana Daniela BACIU; Lucica MIHALTE; Adriana F. SESTRAS; Radu E. SESTRAS

    2010-01-01

    In order to identify cultivars with special decorative value and potential genitors for breeding process, 45 genotypes of Calendula genera were analyzed, belonging to six species: C. officinalis, C. alata, C. arvensis, C. stellata, C. suffruticosa and C. tripterocarpa. The average height of plants varied strongly, from 22.0 cm (C. officinalis cv. Rozovyi Sjurpriz) cm to 84.1 cm (C. tripterocarpa Rupr.). The lowest number of branches per plant was recorded on C. arvensis F. (4.6) and the large...

  18. Variability of decorative traits, response to the Aphis fabae attack and RAPD diversity in different genotypes of Calendula

    Directory of Open Access Journals (Sweden)

    Adriana Daniela BACIU

    2010-12-01

    Full Text Available In order to identify cultivars with special decorative value and potential genitors for breeding process, 45 genotypes of Calendula genera were analyzed, belonging to six species: C. officinalis, C. alata, C. arvensis, C. stellata, C. suffruticosa and C. tripterocarpa. The average height of plants varied strongly, from 22.0 cm (C. officinalis cv. Rozovyi Sjurpriz cm to 84.1 cm (C. tripterocarpa Rupr.. The lowest number of branches per plant was recorded on C. arvensis F. (4.6 and the largest one on C. officinalis LDA (16.4. The average number of flowers per plant ranged from 98.0 (C. suffruticosa Valh. to 2.0 (C. officinalis UK. From among all genotypes, aphids (Aphis fabae have attacked 19 (42.2%, and the results showed that AD% (Attack Degree depend significantly on genotypes. RAPD analysis and phylogenetic dendrogram illustrated the relationship between genotypes and DNA polymorphism exists between the six species. Were found not only close phylogenetic links among cultivars apart of the same specie, but also between different species. C. officinalis A., C. alata UK and C. suffruticosa formed a subgroup similar to the molecular level, but also confirming some phenotypic similarities, these species having the smallest number of petals in the corolla and the highest sensitivity to Aphis fabae attack. The large variability identified in Calendula genotypes allows the selection of potential genitors for new breeding works, with appropriate decorative characteristics and resistance to aphids attack. RAPD analyses and phenotypic study allows hypothesis regarding the success of intra- and inter-specific hybridization, thus facilitating Calendula breeding processes.

  19. Garten-Ringelblume - Calendula officinalis (Asteraceae) : Heilpflanze des Jahres 2009

    OpenAIRE

    Kasielke, Till

    2010-01-01

    Die Garten-Ringelblume ist aus keinem traditionellem Bauern- und Hausgarten wegzudenken. Sie wird im nördlichen Skandinavien ebenso angepflanzt wie am Mittelmeer und ist auf den Höhen des Pamir in Zentralasien so beliebt wie in den weiten Steppen Russlands. Diese außerordentliche Beliebtheit ist neben den geringen Standortansprüchen auf zwei wesentliche Eigenschaften zurückzuführen. Einerseits besitzt die Art zahlreiche Heilkräfte, die zum Teil schon seit der Antike bekannt sind, andererseits...

  20. Mechanical harvesting and cleaning of Calendula officinalis and Dimorphotheca pluvialis

    NARCIS (Netherlands)

    Breemhaar, H.G.; Bouman, A.

    1995-01-01

    From 1990 to 1994 the research institutes of the Agricultural Research Department in the Netherlands (DLO-NL) investigated potential new arable oilseed crops for industrial application as part of the National Oilseeds Programme (NOP). The Institute of Agricultural and Environmental Engineering (IMAG

  1. “IN VITRO” MULTIPLICATION OF CALENDULA OFFICINALIS L.

    Directory of Open Access Journals (Sweden)

    Smaranda Vantu

    2015-09-01

    steps: the shoots were excised and transferred to fresh medium and then rooting of these shoots was achieved on the same medium with 0,02 mg/l benzylaminopurine and 1 mg/l 2,4 dichlorophenoxyacetic acid. The excised shoots were subcultured for roots induction. Regenerated plants were transferred to ex vitro conditions for an acclimatisation period.

  2. Effects of Calendula Essential Oil-Based Cream on Biochemical Parameters of Skin of Albino Rats against Ultraviolet B Radiation.

    Science.gov (United States)

    Mishra, Arun K; Mishra, Amrita; Verma, Anurag; Chattopadhyay, Pronobesh

    2012-09-01

    Reactive oxygen species (ROS) generated from UV-B radiation have the capacity to cause oxidative decomposition which leads to the formation of toxic components as well as lipid peroxidation. Considering this fact, the present study was performed to evaluate the effect of a cream (O/W) containing the essential oil of Calendula officinalis on biochemical parameters of the skin of albino rats against UV-B radiation. The fingerprint analysis of Calendula essential oil was performed by HPLC with special reference to 1,8-cineole and α-pinene. The results indicated that the treatment with creams containing 4% and 5% of Calendula essential oil caused a significant decrease in the malonyldialdehyde level, whereas the levels of catalase, glutathione, superoxide dismutase, ascorbic acid, and the total protein level were significantly increased after 1 month of daily irradiation and treatment when compared to untreated control groups. The results suggest that the cutaneous application of the essential oil of Calendula prevents UV-B-induced alterations in the level of antioxidants in skin tissue. PMID:23008814

  3. ПЕРСПЕКТИВЫ КОМПЛЕКСНОГО ИСПОЛЬЗОВАНИЯ СЫРЬЯ КАЛЕНДУЛЫ ЛЕКАРСТВЕННОЙ ( CALENDULA OFFICINALIS L.)

    OpenAIRE

    Афанасьева, Полина; Куркина, Анна

    2014-01-01

    Проведено фитохимическое исследование по определению содержания суммы флавоноидов и каротиноидов в цветках, семенах, листьях и корнях календулы лекарственной или ноготков лекарственных ( Calendula officinalis L.) сорта «Кальта», культивируемой в Самарской области. Установлено, что наибольшее содержание как флавоноидов (4,37%±0,04%), так и каротиноидов (63,10мг%±3,56%) отмечается в цветках ноготков. Новые подходы к стандартизации позволяют реализовать ряд ресурсоведческих задач в рамках рацион...

  4. Rapid evaluation and comparison of natural products and antioxidant activity in calendula, feverfew, and German chamomile extracts.

    Science.gov (United States)

    Agatonovic-Kustrin, Snezana; Babazadeh Ortakand, Davoud; Morton, David W; Yusof, Ahmad P

    2015-03-13

    The present study describes a simple high performance thin layer chromatographic (HPTLC) method for the simultaneous quantification of apigenin, chamazulene, bisabolol and the use of DPPH free radical as a post-chromatographic derivatization agent to compare the free radical scavenging activities of these components in leaf and flower head extracts from feverfew, German chamomile and marigold from the Asteraceae family. Feverfew (Tanacetum parthenium) leaves have been traditionally used in the treatment of migraine with parthenolide being the main bioactive compound. However, due to similar flowers, feverfew is sometimes mistaken for the German chamomile (Matricaria recutita). Bisabolol and chamazulene are the main components in chamomile essential oil. Marigold (Calendula officinalis) was included in the study for comparison, as it belongs to the same family. Parthenolide was found to be present in all leaf extracts but was not detected in calendula flower extract. Chamazulene and bisabolol were found to be present in higher concentrations in chamomile and Calendula flowers. Apigenin was detected and quantified only in chamomile extracts (highest concentration in flower head extracts). Antioxidant activity in sample extracts was compared by superimposing the chromatograms obtained after post-chromatographic derivatization with DPPH and post-chromatographic derivatization with anisaldehyde. It was found that extracts from chamomile flower heads and leaves have the most prominent antioxidant activity, with bisabolol and chamazulene being the most effective antioxidants. PMID:25666499

  5. Calendula

    Science.gov (United States)

    ... ModerateBe cautious with this combination.Talk with your health provider. ... these include 5-HTP, calamus, California poppy, catnip, hops, Jamaican dogwood, kava, St. John's wort, skullcap, valerian, yerba mansa, and others.

  6. Salvia officinalis in dentistry

    Directory of Open Access Journals (Sweden)

    Nikhita Narayanan

    2015-01-01

    Full Text Available Salvia officinalis is a medicinal herb used as an cosmetic, flavoring agent, It has antibacterial, antifungal, anticaries, antiplaque, antiviral, astringent, and other useful properties, it is also used in dental practice for the management of periodontal disease and to prevent halitosis. The objective of this article is to highlight various uses of S. officinalis in the dental field along with its use in medical problems.

  7. Inibição do crescimento micelial de Cercospora calendulae Sacc. por extratos de plantas medicinais

    Directory of Open Access Journals (Sweden)

    J.M. Nascimento

    2013-01-01

    Full Text Available O uso de extratos e óleos essenciais de plantas medicinais tem sido amplamente estudado no controle de doenças de plantas. O objetivo da realização do presente trabalho foi avaliar o efeito de extratos de Ruta graveolens L., Mentha x villosa, Calendula officinalis L., Momordica charantia L., Symphytum officinale L., Ageratum conyzoides L. e Ricinus comunis L., nas concentrações de 0, 250, 500, 1000, 2000, 5000 e 10000 mg L-1, sobre a inibição do crescimento micelial de Cercospora calendulae Sacc. in vitro. Os extratos foram obtidos por infusão. O experimento foi desenvolvido no Laboratório de Fitopatologia da FCA/UFGD, estado de Mato Grosso do Sul, em delineamento inteiramente casualizado, esquema fatorial 7 extratos x 7 concentrações, com seis repetições. Foi detectado efeito dos extratos e suas concentrações sobre o crescimento do fungo, sendo a interação significativa. Os extratos de calêndula, arruda, hortelã e melão de São Caetano, nas maiores concentrações resultaram em maiores porcentagens de inibição, próximas de 100%, 30%, 35% e 40%, respectivamente, a 10000 mg L-1.

  8. Estimativa do filocrono em calêndula Estimating the phyllochron in calendula

    Directory of Open Access Journals (Sweden)

    Jana Koefender

    2008-08-01

    Full Text Available O objetivo deste trabalho foi estimar o filocrono em plantas de calêndula (Calendula officinalis L. cultivadas em diferentes épocas de semeaduras. Realizaram-se três épocas de semeaduras: 06/04/2005, 23/06/2005 e 03/10/2006, no interior de uma estufa plástica com área de 240m2, instalada no campus da Universidade Federal de Santa Maria, UFSM. A semeadura foi realizada em vasos plásticos com capacidade volumétrica para cinco litros. O delineamento experimental foi inteiramente casualizado com 320 vasos em cada época, sendo distribuído em quatro fileiras de 80 vasos cada. Em uma planta de oito vasos de cada quatro fileiras, fez-se a contagem, semanalmente, do número de folhas emergidas da haste principal e na primeira haste lateral. Os dados do número de folhas foram correlacionados com a soma térmica diária acumulada, considerando-se uma temperatura base de 8°C. Constatou-se que o filocrono variou com a época de semeadura, sendo o menor valor igual 15,9°C dia folha-1, obtido na 2ª época de semeadura e o maior, igual a 24,5°C dia folha-1, na 1ª época na haste principal e 48,9°C dia folha-1 na semeadura de outubro na primeira haste lateral.The objective of this study was to estimate the phyllochron in calendula (Calendula officinalis L. grown in different sowing dates. Three sowing dates (06/04/2005, 23/06/2005 and 03/10/2006 were performed inside a 240m2 plastic greenhouse at the Campus of the Federal University of Santa Maria, RS, Brazil. Sowing was in five liter plastic pots. The experimental design was a completely randomized with 320 pots on each sowing date and rows with 80 pots. The number of leaves in the main stem and oi the first lateral branch was counted on a weekly basis in one plant of eight pots. The number of leaves data was regressed against accumulated thermal time, assuming a base temperature of 8oC. The main stem phyllochron varied with sowing date, with the lowest value (15.9°C day leaf -1 obtained on

  9. [Brief explanation of shi lin (urolithic stranguria)].

    Science.gov (United States)

    Li, Yuan-yuan

    2010-05-01

    Shi lin (urolithic stranguria) is a kind of stranguria, manifested as pain occurred when pissing and sand-like in the urine, developed from the early concept of shi long. This name of disease was appeared firstly in Shen nong ben cao jing (Shennong's Classic of Materia Medica), and then, the disease name of sha lin, sha shi lin and sha lin were derived from it by the Song Dynasty. It was said that the disease with sha (sand-like) was easy to treat, and with shi (stone-like) was difficult to cure. This disease was also happened in the children, and the reasons were the same with ones of the adult. Furthermore, there was a name of disease, xue sha lin, which was a kind of woman disease and had nothing to do with shi lin. PMID:21029715

  10. LINS Curve in Romanian Economy

    Directory of Open Access Journals (Sweden)

    Emilian Dobrescu

    2016-02-01

    Full Text Available The paper presents theoretical considerations and empirical evidence to test the validity of the Laffer in Narrower Sense (LINS curve as a parabola with a maximum. Attention is focused on the so-called legal-effective tax gap (letg. The econometric application is based on statistical data (1990-2013 for Romania as an emerging European economy. Three cointegrating regressions (fully modified least squares, canonical cointegrating regression and dynamic least squares and three algorithms, which are based on instrumental variables (two-stage least squares, generalized method of moments, and limited information maximum likelihood, are involved.

  11. Developements of Calendula officinalis Oil%金盏菊油开发利用

    Institute of Scientific and Technical Information of China (English)

    郭玉宝; 裘爱泳; 徐霞

    2002-01-01

    金盏菊富含多种功能性化合物,如类黄酮、三萜烯皂甙、类胡萝卜素、维生素E及共轭十八碳三烯酸等.本文主要综述国内外对金盏菊油研究现状和可能的工业应用,并展望其开发前景.

  12. Effect of biostimulant application on production and flavonoid content of marigold (Calendula officinalis L.

    Directory of Open Access Journals (Sweden)

    Vivian Pupo de Oliveira Machado

    2014-12-01

    Full Text Available The production of medicinal plants as raw material for industry must associate quality with biomass formation and, with this purpose, the application of plant growth regulators has been studied in these crops. The objective of this study was to evaluate the effect of a biostimulant on growth, inflorescence production and flavonoid content in marigold. The experiment was conducted in a greenhouse and the treatments consisted of increasing doses of the biostimulant (0, 3, 6, 9, 12 and 15 mL L-1 applied by foliar spraying in ten consecutive applications. The experiment was arranged in a completely randomized design, with six treatments and ten repetitions. The number of leaves and flowerheads and dry matter of roots increased linearly with increasing doses of the growth promoter, with 20%, 36.97% and 97.28% increases, respectively, compared with the control. The total dry mass and shoot dry mass showed maximum values at the highest dose tested of 15 mL L-1 (with increases of 40.09% and 46.30%, respectively. Plant height and flavonoid content reached the highest values at a dose of 6 mL L-1. The biostimulant promoted the development of marigold and positively influenced the synthesis of the secondary compound of medicinal interest. Among the tested doses, the application of rates between 6 and 9 mL L-1 of the biostimulant is recommended for more efficient large-scale production of marigold.

  13. Determination of total lipids and characterization of marigold flower extracts (Calendula officinalis

    Directory of Open Access Journals (Sweden)

    Novković Vesna M.

    2005-01-01

    Full Text Available Bioactive extracts from marigold flower are important ingredients for parapharmaceutical and cosmetic preparations. Their antiflogistic holeretic.antimicrobic, antidermatic and anticancer effects are due to the presence of flavonoids, carotenoids, etheric oils, and terpenoids. This study presents the results of spectrophotometric investigation for the overall carotene content calculated as (3-caroten (442 nm, visual and physico-chemical characteristics according to Ph.Jug. V in oil extracts of marigold flower obtained by maceration (room temperature and 70°C and percolation (room temperature with olive oil and sunflower oil by original procedures.The largest content of (3-carotene (57.5 mg/kg of oil extracts is in the oil extract obtained by maceration for 72 hours with olive oil (solvomodulus 1:5 m/m at room temperature.

  14. Uso da adubação orgânica e cobertura morta na cultura da calêndula (Calendula officinalis L.) Use of organic fertilization and mulching in the production of calendula (Calendula officinalis L.)

    OpenAIRE

    C.B.O. Araújo; Santos, A. M.; L. A. Fernandes; Martins, E. R.; R.A. Sampaio; Costa, C. A.; G.L.D. Leite

    2009-01-01

    A calêndula representa fonte alternativa econômica principalmente para a agricultura familiar. O objetivo do presente trabalho foi avaliar os efeitos da adubação orgânica e do uso da cobertura morta sobre a produção de biomassa e teor de flavonóides totais em plantas de calêndula. O experimento foi realizado no Campus de Montes Claros da Universidade Federal de Minas Gerais, utilizando-se o delineamento em blocos casualizados em esquema fatorial 2x2x4 com quatro repetições: duas épocas de col...

  15. Analysis list: lin-42 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-42 + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-42.1.tsv h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-42.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin...-42.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-42..tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/.gml ...

  16. Expression of recombinant protein Lin-7%重组Lin-7蛋白的表达

    Institute of Scientific and Technical Information of China (English)

    杨煜; 董辉; 劳凤学; 徐桂月; 张学军; 柳忠辉

    2002-01-01

    目的:表达具有PDZ功能区的Lin-7蛋白,并制备抗Lin-7蛋白的特异性抗体.方法:采用质粒构建表达重组GST-Lin-7蛋白,SDS-PAGE分析表达蛋白;以GST-Lin-7蛋白为抗原免疫家兔,制备抗Lin-7多克隆抗体,ELISA法检测抗体特异性.结果:重组GST-Lin-7蛋白呈分泌型表达于胞浆内;制备的抗Lin-7抗体与GST、ARIPs等蛋白无交叉反应.结论:成功地构建了Lin-7蛋白表达质粒,并制备了抗Lin-7 的特异性抗体.

  17. Analysis list: lin-37 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-37 Embryo,Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin...-37.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-37.5.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/ce10/target/lin-37.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-37.Embryo....tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-37.Larvae.tsv http:

  18. 金盏菊花类胡萝卜素的提取及性质研究%Study on the Extraction and Property of Carotenoids from Colendula Officinalis L.Flowers

    Institute of Scientific and Technical Information of China (English)

    胡晓丹; 谢笔钧

    2001-01-01

    主要对中国产金盏菊花中类胡萝卜素的提取、纯化及其稳定性进行了研究,为金盏菊花类胡萝卜素的提取和应用提供了科学依据。%The paper mainly studied the extraction, purification, stability of carotenoids from calendula officinalis L. Flowers in China, which provides scientfic basis for the extraction and application of the carotenoids.

  19. Dr. Lin Zhu's Clinical Experience in Treating Mental Disorders

    Institute of Scientific and Technical Information of China (English)

    Ma Xueqing; Wang Xinzhong

    2005-01-01

    @@ Mental disorders are mainly caused by the emotional factors. Chief physician Lin Zhu, a famous TCM doctor in Beijing, is very good at treating this kind of disorders. The following is a summary of Prof. Lin's experience in this aspect.

  20. Richard Lin Leads the Energy Evolution

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ Richard Lin Helms Dynamotive in energy era At the very beginning of 2008, a big event occurred in the international market and shocked the world: On January 2, the first trade day in the New Year, the crude price jumped to US$100 per barrel, creating a historically new high and challenging people's bearing capacity.More and more,urgent voices were heard.

  1. Lin Hairong:A Quiet Commentary

    Institute of Scientific and Technical Information of China (English)

    Jade; Franklin

    2007-01-01

    In looking at the works of Lin Hairong, one senses that the artist is seeking to capture life's tranquil moments; those fleeting glimpses of halcyon clarity and peace that remain uncontaminated by the frenetic pace of daily existence. Moments such as these have become few and far between however, in an age that the artist feels "is too quick to change".

  2. The Lin28/let-7 Axis Regulates Glucose Metabolism

    NARCIS (Netherlands)

    Zhu, Hao; Shyh-Chang, Ng; Segre, Ayellet V.; Shinoda, Gen; Shah, Samar P.; Einhorn, William S.; Takeuchi, Ayumu; Engreitz, Jesse M.; Hagan, John P.; Kharas, Michael G.; Urbach, Achia; Thornton, James E.; Triboulet, Robinson; Gregory, Richard I.; Altshuler, David; Daley, George Q.

    2011-01-01

    The let-7 tumor suppressor microRNAs are known for their regulation of oncogenes, while the RNA-binding proteins Lin28a/b promote malignancy by inhibiting let-7 biogenesis. We have uncovered unexpected roles for the Lin28/let-7 pathway in regulating-metabolism. When overexpressed in mice, both Lin28

  3. Analysis list: lin-52 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-52 Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-52....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-52.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin...-52.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-52.Larvae.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  4. Analysis list: lin-53 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-53 Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-53....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-53.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin...-53.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-53.Larvae.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  5. Analysis list: lin-54 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-54 Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-54....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-54.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin...-54.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-54.Larvae.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  6. Analysis list: lin-11 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-11 Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-11....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-11.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin...-11.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-11.Larvae.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  7. Analysis list: lin-39 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-39 Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-39....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-39.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin...-39.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-39.Larvae.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  8. Analysis list: lin-9 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-9 Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-9.1....tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-9.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin...-9.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-9.Larvae.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  9. Analysis list: lin-61 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-61 Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-61....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-61.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin...-61.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-61.Larvae.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  10. Analysis list: lin-13 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-13 Embryo,Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/...lin-13.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-13.5.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/ce10/target/lin-13.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-13.Embryo....tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-13.Larvae.tsv http://dbarchive.bioscien...cedbc.jp/kyushu-u/ce10/colo/Embryo.gml,http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  11. Analysis list: lin-35 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available lin-35 Adult,Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-35.1.tsv http:...//dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/lin-35.5.tsv http://dbarchive.bioscienced...bc.jp/kyushu-u/ce10/target/lin-35.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-35.Adult.tsv,http:...//dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/lin-35.Larvae.tsv http://...dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Adult.gml,http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

  12. Effect of putrescine and salinity on morphological and biochemical traits and pigment content of marigold plant (Calendula officinalis L.

    Directory of Open Access Journals (Sweden)

    2015-06-01

    Full Text Available In order to evaluate the effects of salt stress and putrescine on some morphological and biochemical traits and pigment content of pot marigold plant, an experiment was conducted under greenhouse conditions based on completely randomized design, using four levels of sodium chloride (1, 3, 6 and 9 dS/m and three levels of putrescine (0, 1 and 2 mM. Sodium chloride was applied on the soil, while putrescine solution was sprayed on the leaves. Results showed that salt stress reduced all growth parameters and pigment content significantly, while increased the amount of reduced sugars in leaves and roots. Application of putrescine had significant effect on all biochemical parameters and pigment content; but had no effect on the number and diameter of flowers. Spraying of 2 mM putrescine, under salt stress level of 9 dS/m, increased fresh flower weight, shoot dry weight, chlorophyll a, total chlorophyll, leaf cartenoides and reduced sugars by 29, 27, 35, 32, 17 and 15 percent, respectively, compared to control treatment. Based on the results of this experiment, it was concluded that application of putrescine improves the growth, biochemical traits and pigment content of marigold plant under salt stress conditions.

  13. 金盏菊色素的提取及分离%Calendula officinalis Pigment Extraction and Separation

    Institute of Scientific and Technical Information of China (English)

    马丽英; 彭军强

    2013-01-01

    采用纸层析、硅胶板层析和柱层析分别对水和酒精提取的金盏菊色素进行分离.结果表明:水提取的色素较为纯净,只有一种黄色色素;而酒精可将金盏菊中的类胡萝卜素提取出来.水提取的色素不稳定,易被光分解;而酒精提取的色素光稳定性较好.酒精提取的色素用展开剂苯∶乙醚∶丙酮=1∶1∶1及丙酮∶乙醚∶氯仿=1∶1∶1分离效果较好.

  14. 金盏花配方施肥效应研究%Studies on Formula Fertilization Effect to Calendula officinalis

    Institute of Scientific and Technical Information of China (English)

    朱亚; 赵永平; 何庆祥; 周彦芳; 刘强

    2011-01-01

    采用"3414"试验设计,对氮磷钾肥不同配施比例与金盏花产量关系进行研究.结果表明:不同氮、磷、钾肥配拖对鲜花产量影响达显著水平,各施肥处理的鲜花产量分别比不施肥处理增产3 045.9-6 414.2kg/hm2;缺氮对产量影响最大,磷肥次之,钾肥最小;最佳产量施肥量为:N 158.54 kg/hm2,P2O5 126.61kg/hm2,K2O 62.80kg/hm2,最佳产量为33 722.58 kg/hm2.

  15. Stability study of calendula officinalis yellow pigment%金盏菊桔费色素稳定性研究

    Institute of Scientific and Technical Information of China (English)

    李清芳; 马成仓; 濮云飞

    2001-01-01

    研究了金盏菊桔黄色素的溶解性,对光、热的稳定性及几种常用食品添加剂对金盏菊桔黄色素稳定性的影响,并分析了酸、碱、几种常见离子对其稳定性的作用,为生产上使用色素提供依据.

  16. HPLC Fingerprint of Calendula officinalis Flower%金盏花HPLC特征指纹图谱研究

    Institute of Scientific and Technical Information of China (English)

    邢占芬; 成洪达; 张平平; 宫磊; 马丽娅

    2014-01-01

    目的:建立金盏花的HPLC特征指纹图谱,为科学评价其质量提供方法.方法:以Hypersil ODS C18柱(250 mm×4.6 mm,5μm)为色谱柱,乙腈-水为流动相进行梯度洗脱,流速1.0 mL/min,检测波长220 nm,柱温35cc;采用中药色谱指纹图谱评价系统进行相似度分析.结果:确定了含有11个色谱峰的特征指纹图谱,不同产地金盏花特征图谱相似度均在0.90以上.结论:建立的方法简单、可靠,可用于金盏花的鉴别和质量控制.

  17. Complete genome sequence of Bacillus amyloliquefaciens strain Co1-6, a plant growth-promoting rhizobacterium of Calendula officinalis

    Energy Technology Data Exchange (ETDEWEB)

    Koeberl, Martina; White, Richard A.; Erschen, Sabine; Spanberger, Nora; El-Arabi, Tarek F.; Jansson, Janet K.; Berg, Gabriele

    2015-08-13

    The genome sequence of Bacillus amyloliquefaciens strain Co1-6, a plant growth-promoting rhizobacterium (PGPR) with broad-spectrum antagonistic activities against plant pathogenic fungi, bacteria and nematodes, consists of a single 3.9 Mb circular chromosome. The genome reveals genes putatively responsible for its promising biocontrol and PGP properties.

  18. The seamy side of natural medicines: contact sensitization to arnica (Arnica montana L.) and marigold (Calendula officinalis L.).

    Science.gov (United States)

    Reider, N; Komericki, P; Hausen, B M; Fritsch, P; Aberer, W

    2001-11-01

    Medical remedies of plant origin have gained increasing popularity in recent years. Both anaphylactic and eczematous allergic reactions are on the rise, accordingly. Arnica and marigold, both of the Compositae family, are in widespread use, but only limited data are available on their allergenic potential. We tested 443 consecutive patients, in addition to the European standard and other series, with Compositae mix, sesquiterpene lactone mix, arnica, marigold, and propolis. 5 subjects ( approximately 1.13%) reacted to arnica, 9 ( approximately 2.03%) to marigold. The Compositae mix was positive in 18 cases ( approximately 4.06%). Among them were 3 out of 5 individuals with a sensitization to arnica, and 4 out of 9 who reacted to marigold. Sensitization to arnica and marigold was often accompanied by reactions to nickel, Myroxylon Pereirae resin, fragrance mix, propolis, and colophonium. We conclude that Compositae allergy contributes significantly to the epidemiology of contact dermatitis and that sensitization to arnica and marigold cannot be assessed by testing with the Compositae or sesquiterpene mix alone. As extracts of these plants are frequently used in occupational and cosmetic products, patch testing with additional plant extracts or adjustment of the commercial Compositae mix to regional conditions is recommended.

  19. Effect of Calendula officinalis hydroalcoholic extract on passive avoidance learning and memory in streptozotocin-induced diabetic rats

    Directory of Open Access Journals (Sweden)

    Shirin Moradkhani

    2015-01-01

    Conclusion: Taken together, CO extract can improve PAL and memory impairments in STZ-diabetic rats. This improvement may be due to its antioxidant, anticholinergic activities or its power to reduce hyperglycemia.

  20. Complete Genome Sequence of Bacillus amyloliquefaciens Strain Co1-6, a Plant Growth-Promoting Rhizobacterium of Calendula officinalis.

    Science.gov (United States)

    Köberl, Martina; White, Richard A; Erschen, Sabine; Spanberger, Nora; El-Arabi, Tarek F; Jansson, Janet K; Berg, Gabriele

    2015-01-01

    The genome sequence of Bacillus amyloliquefaciens strain Co1-6, a plant growth-promoting rhizobacterium (PGPR) with broad-spectrum antagonistic activity against plant-pathogenic fungi, bacteria, and nematodes, consists of a single 3.9-Mb circular chromosome. The genome reveals genes putatively responsible for its promising biocontrol and PGP properties. PMID:26272562

  1. Complete Genome Sequence of Bacillus amyloliquefaciens Strain Co1-6, a Plant Growth-Promoting Rhizobacterium of Calendula officinalis

    OpenAIRE

    Köberl, Martina; White, Richard A.; Erschen, Sabine; Spanberger, Nora; El-Arabi, Tarek F.; Jansson, Janet K.; Berg, Gabriele

    2015-01-01

    The genome sequence of Bacillus amyloliquefaciens strain Co1-6, a plant growth-promoting rhizobacterium (PGPR) with broad-spectrum antagonistic activity against plant-pathogenic fungi, bacteria, and nematodes, consists of a single 3.9-Mb circular chromosome. The genome reveals genes putatively responsible for its promising biocontrol and PGP properties.

  2. 小鼠骨髓CD90+、CD34+、CD117+和Sca-1+细胞的肝干细胞潜能比较%COMPARISON OF THE HEPATIC STEM CELL POTENTIAL OF GD90 + LIN-, CD34 + LIN-, CD117 + LIN-AND SCA-1+LIN-AND SCA-1+LIN-BONE MARROW CELLS IN Mice

    Institute of Scientific and Technical Information of China (English)

    廖彩仙; 唐浩; 周杰; 苏俊; 张春兴; 张守华

    2006-01-01

    目的 比较的小鼠CD90+Lin-、C34+Lin-、CD117+Lin-和Sca-1+Lin-骨髓细胞的肝干细胞潜能.方法 供体为纯系BALB/C雄性小鼠,应用免疫磁珠分选法获取CD90+Lin-、CD34+Lin-、CD117+Lin-和Sca-1+Lin-骨髓细胞.受体为35Gy全肝照射处理后的同龄同系BALB/C雌性小鼠,A、B、C、D组小鼠分别接受CD90+Lin-、CD34+Lin-、CD117+Lin-、Sca-1+Lin-骨髓细胞移植.移植后30天活杀小鼠,取肝进行Y染色体原位分子杂交与白蛋白免疫组织化学联合检测.结果 各组小鼠肝组织中都可以检测到Y染色体和白蛋白均呈阳性的双阳性细胞,C组的双阳性细胞数量显著多于其它各组.结论 CD90+Lin-、CD34+Lin-、CD117+Lin-、Sca-1+Lin-骨髓细胞都具有肝干细胞潜能,其中CD117+Lin-骨髓细胞的肝干细胞潜能最大、形成肝细胞的能力量强.

  3. On the low regularity of the Benney-Lin equation

    Science.gov (United States)

    Chen, Wengu; Li, Junfeng

    2008-03-01

    We consider the low regularity of the Benney-Lin equation ut+uux+uxxx+[beta](uxx+uxxxx)+[eta]uxxxxx=0. We established the global well posedness for the initial value problem of Benney-Lin equation in the Sobolev spaces for 0[greater-or-equal, slanted]s>-2, improving the well-posedness result of Biagioni and Linares [H.A. Biaginoi, F. Linares, On the Benney-Lin and Kawahara equation, J. Math. Anal. Appl. 211 (1997) 131-152]. For s<-2 we also prove some ill-posedness issues.

  4. 原位杂交检测lin-28 mRNA在 Caenorhabditis elegans中的分布%Distribution of lin-28 mRNA in Wild-type and Mutants of C. elegans Via in situ Hybridization

    Institute of Scientific and Technical Information of China (English)

    杨玉荣; 郑卫玲

    2008-01-01

    利用原位杂交检测了异时性基因lin-28 mRNA在野生型和lin-4,lin-14突变体以及lin-4,lin-14双突变体中的分布,发现lin-28 mRNA在胚胎和成虫生殖腺中存在.还发现lin-4突变不改变lin-28 mRNA分布,证明了lin-28表达独立于lin-4抑制通路的存在.

  5. 原位杂交检测历lin-28mRNA在Caenorhabditis elegans中的分布

    Institute of Scientific and Technical Information of China (English)

    杨玉荣; 郑卫玲

    2008-01-01

    利用原位杂交检测了异时性基因lin-28mRNA在野生型和lin-4,lin-14突变体以及lin-4,lin-14双突变体中的分布,发现lin-28mlLNA在胚胎和成虫生殖腺中存在.还发现lin-4突变不改变lin-28mlLNA分布,证明了lin-28表达独立于lin-4抑制通路的存在.

  6. In vitro expansion of Lin{sup +} and Lin{sup −} mononuclear cells from human peripheral blood

    Energy Technology Data Exchange (ETDEWEB)

    Norhaiza, H. Siti; Zarina, Z. A. Intan; Hisham, Z. A. Shahrul [School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Selangor (Malaysia); Rohaya, M. A. W. [Department of Orthodontics, Faculty of Dentistry, Universiti Kebangsaan Malaysia, 50300, Kuala Lumpur (Malaysia)

    2013-11-27

    Haematopoietic stem cells (HSCs) are used in the therapy of blood disorders due to the ability of these cells to reconstitute haematopoietic lineage cells when transplanted into myeloablative recipients. However, substantial number of cells is required in order for the reconstitution to take place. Since HSCs present in low frequency, larger number of donor is required to accommodate the demand of transplantable HSCs. Therefore, in vitro expansion of HSCs will have profound impact on clinical purposes. The aim of this study was to expand lineage negative (Lin{sup −}) stem cells from human peripheral blood. Total peripheral blood mononuclear cells (PBMNCs) were fractionated from human blood by density gradient centrifugation. Subsequently, PBMNCs were subjected to magnetic assisted cell sorter (MACS) which depletes lineage positive (Lin{sup +}) mononuclear cells expressing lineage positive markers such as CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a to obtained Lin{sup −} cell population. The ability of Lin{sup +} and Lin{sup −} to survive in vitro was explored by culturing both cell populations in complete medium consisting of Alpha-Minimal Essential Medium (AMEM) +10% (v/v) Newborn Calf Serum (NBCS)+ 2% (v/v) pen/strep. In another experiment, Lin{sup +} and Lin{sup −} were cultured with complete medium supplemented with 10ng/mL of the following growth factors: stem cell factor (SCF), interleukin (IL)-3, granulocyte-macrophage colony stimulating factor (GM-CSF), 2IU/mL of Erythropoietin (Epo) and 20ng/mL of IL-6. Three samples were monitored in static culture for 22 days. The expansion potential was assessed by the number of total viable cells, counted by trypan blue exclusion assay. It was found that Lin{sup +} mononuclear cells were not able to survive either in normal proliferation medium or proliferation medium supplemented with cytokines. Similarly, Lin{sup −} stem cells were not able to survive in proliferation medium however

  7. CONGRATULATIONS TO ACADEMICIAN LIN QUN ON HIS 70th BIRTHDAY

    Institute of Scientific and Technical Information of China (English)

    Institute of Computational Mathematics; Scientific/Engineering Computing

    2006-01-01

    @@ This special issue of the Journal of Computational Mathematics is dedicated to Professor Qun Lin, an outstanding mathematician and a Member of Chinese Academy of Sciences (CAS), on the occasion of his seventieth birthday.

  8. The Policy Issues in Lin an Modei Forest of China

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Based on the analysis on policy such as property rights, tax and administration fee, harvesting and circulation of forest products in Lin 'an Modei Forest, some policy issues in the development of Lin 'an Model Forest towards sustainable forest management were raised: (1) The level of timber tax and fee is quite high and there are many kinds of taxes and fees such as fees for forest silviculture, forestry maintenance, forest protection, city construction, town-service, additional fee for education and o...

  9. Calendula oil processing : seed classification, oil extraction, refining process development and oil quality aspects

    NARCIS (Netherlands)

    Janssens, R.J.J.

    2000-01-01

    The difference in Calendula oil quality from fractions obtained after seed classification is enormous. The oil quality varies from excellent to very poor, according to important aspects such as in the hulls and dust fraction, high free fatty acid values (13% vs. 0.6%) are found. This can be explaine

  10. EFECTO DE EXTRACTOS ACUOSOS DE Helianthus annuus Lin. SOBRE EL CRECIMIENTO DE Solanum lycopersicum Lin.

    Directory of Open Access Journals (Sweden)

    Yaniuska González Perigó

    2015-01-01

    Full Text Available Con el objetivo de determinar el efecto de extractos acuosos de Helianthus annuus Lin. sobre el crecimiento de Solanum lycopersicum Lin, se tomaron muestras de raíz y hojas de plantas recolectadas de un sistema de policultivo ubicado en un agroecosistema montañoso. Los extractos acuosos de girasol se obtuvieron a partir de raíz y hojas de plantas recolectadas durante dos años. Se evaluaron los efectos de estos extractos sobre la germinación, longitud de la radícula y del hipocótilo de semillas de tomate certificadas, para ello se ejecutaron nueve tratamientos con cuatro repeticiones dispuestos en placas Petri. Los extractos de raíz de girasol a los 15, 30 y 45 días y de hojas a los 75 y 90 días de desarrollo inhibieron la germinación de las semillas de tomate y el crecimiento de la radícula y el hipocótilo. Los extractos de raíz de girasol con 75 y 90 días, estimularon el crecimiento de la radícula y del hipocótilo del tomate. Estos resultados demuestran que el girasol produce sustancias químicas que inhiben el crecimiento del tomate y no se recomienda asociar estos dos cultivos de forma simultánea.

  11. Supercritical carbon dioxide extraction of antioxidants from rosemary (Rosmarinus officinalis L.) and sage (Salvia officinalis L.)

    OpenAIRE

    JASNA IVANOVIĆ; SONJA ĐILAS; MILKA JADRANIN; VLATKA VAJS; NADA BABOVIĆ; SLOBODAN PETROVIĆ; IRENA ŽIŽOVIĆ

    2009-01-01

    The aim of the present study was to isolate and characterize antioxidant extracts obtained from dried leaves of rosemary (Rosmarinus officinalis L.) and sage (Salvia officinalis L.), originating from the southern Balkan Region. The antioxidant fraction was isolated from the plant material by supercritical carbon dioxide (SC-CO2) fractional extraction under a pressure of 30 MPa and at temperatures of 40 and 100 °C. In the present study, kinetic data and yields of antioxidant extracts obtained ...

  12. Borago officinalis L. (fotografía)

    OpenAIRE

    Mallor Giménez, Cristina

    2013-01-01

    Front cover: Borago officinalis L., one of the priority crop wild relative species of Spain. Cultivated forms are grown along the Ebro Valley in the northeast. Its basal leaf petioles are used as a vegetable. Borage is also a valuable medicinal plant with a high content of gamma linolenic acid

  13. [Chemical constituents of Morinda officinalis How].

    Science.gov (United States)

    Li, S; Ouyang, Q; Tan, X; Shi, S; Yao, Z

    1991-11-01

    This paper reports the identification of four compounds isolated from the cortex of Morinda officinalis growing in Guangdong Province. These compounds are beta-sitosterol (I), 2-methyl-anthraquinone(II), rubiadin-1-methyl ether(III) and 24-ethylcholesterol(VI). Compounds II and VI are isolated from Morinda Genus for the first time. PMID:1804172

  14. Neuroprotective and neurological properties of Melissa officinalis

    DEFF Research Database (Denmark)

    López, Víctor; Martín, Sara; Gómez-Serranillos, Maria Pilar;

    2009-01-01

    Melissa officinalis has traditionally been used due to its effects on nervous system. Both methanolic and aqueous extracts were tested for protective effects on the PC12 cell line, free radical scavenging properties and neurological activities (inhibition of MAO-A and acetylcholinesterase enzymes...

  15. Development of mouthwash with Rosmarinus officinalis extract

    Directory of Open Access Journals (Sweden)

    Isabela Moreira Baumgratz de Paula

    2014-12-01

    Full Text Available Rosmarinus officinalis, which belongs to the Lamiaceaefamily, is a species of medicinal flora with therapeutic properties. In order to exploit the benefits of these properties, a mouthwash formulation was developed, with careful selection of raw materials to meet pharmacotechnical requirements. Extracts of the plant were incorporated into a mouthwash, which was shown to have inhibitory action in vitro against the micro-organisms commonly found in periodontics. Controls for assessing the quality of the drugs were carried out, quantifying phenols and flavonoids as chemical markers. Mouthwash solutions were formulated containing 0.1, 5 and 10% ethanol extract of R. officinalis; and 0.05, 5 and 10% of the hexane fraction of R. officinalis. In order to evaluate synergism, ethanol extract and hexane fraction were also added to formulations containing 0.05% sodium fluoride and 0.12% chlorhexidine digluconate. These formulations were assessed for inhibitory effect against the specific microorganisms involved in the process of bacterial plaque formation, S. mutans(ATCC25175 and C. albicans(ATCC 10231, frequently found in cases of oral infections. The agar diffusion method was used to evaluate the inhibitory activity of extracts and formulations. All mouthwash solutions displayed inhibitory activity having higher sensitivity to S. mutansfor the 5% ethanol extract+0.05% sodium fluoride, and greater sensitivity to C. albicansfor the 10% hexane fraction. Results were characterized by the appearance of a growth inhibition halo, justifying the utilization and association of extracts of R. officinalis.

  16. The Analysis of Lin Yutang’s Humor

    Institute of Scientific and Technical Information of China (English)

    陈晓琴

    2014-01-01

    Lin Yutang is called the master humorist in China. He advocates humor and spiritual literature in his maga-zine Lun Yu (Analects) which attracts essays and readership. His informal but polished style in both Chinese and English makes him one of the most influential writers of his generation, and his compilations and translations of classic Chinese texts into English are bestsellers in the West. Humor is a distinctive feature in his works. In his book, he uses humor as he sees appropriate, and through humor he criticizes social realities, depicts characters and introduces original expressions, etc. His humor can be related to some aspects in his life. In this paper, I will briefly analysis Lin's sense of humor and how Lin' applies humor in his works.

  17. Aberrant regulation of the LIN28A/LIN28B and let-7 loop in human malignant tumors and its effects on the hallmarks of cancer.

    Science.gov (United States)

    Wang, Tianzhen; Wang, Guangyu; Hao, Dapeng; Liu, Xi; Wang, Dong; Ning, Ning; Li, Xiaobo

    2015-01-01

    RNA binding proteins (RBPs) and microRNAs (miRNAs) are two of the most important post-transcriptional regulators of gene expression, and their aberrant expression contributes to the development of human malignancies. Let-7, one of the most well-known tumor suppressors, is frequently down-regulated in a variety of human cancers. The RBP LIN28A/LIN28B, a direct target of the let-7 family of miRNAs, is an inhibitor of let-7 biogenesis and is frequently up-regulated in cancers. Aberrant regulation of the LIN28A/LIN28B and let-7 loop in human malignant tumors is reportedly involved in cancer development, contributing to cellular proliferation, cell death resistance, angiogenesis, metastasis, metabolism reprogramming, tumor-associated inflammation, genome instability, acquiring immortality and evading immune destruction. In this review, we summarized the mechanisms of LIN28A/LIN28B and let-7 loop aberrant regulation in human cancer and discussed the roles and potential mechanisms of the LIN28A/LIN28B and let-7 loop in regulating the hallmarks of cancer. The crosstalk between LIN28A/LIN28B and let-7 loop and certain oncogenes (such as MYC, RAS, PI3K/AKT, NF-κB and β-catenin) in regulating hallmarks of cancer has also been discussed. PMID:26123544

  18. General Lin-Maldacena solutions and PWMM Instantons from supergravity

    OpenAIRE

    van Anders, Greg

    2007-01-01

    We use the Lin-Maldacena prescription to demonstrate how to find the supergravity solutions dual to arbitrary vacua of the plane wave matrix model and maximally supersymmetric Yang-Mills theory on RxS^2, by solving the auxiliary electrostatics problem. We then apply the technique to study instantons at strong coupling in the matrix model.

  19. General Lin-Maldacena solutions and PWMM instantons from supergravity

    International Nuclear Information System (INIS)

    We use the Lin-Maldacena prescription to demonstrate how to find the supergravity solutions dual to arbitrary vacua of the plane wave matrix model and maximally supersymmetric Yang-Mills theory on R x S2, by solving the auxiliary electrostatics problem. We then apply the technique to study instantons at strong coupling in the matrix model

  20. MEDICINAL USES AND PHYTOCONSTITUENTS OF PAEONIA OFFICINALIS

    Directory of Open Access Journals (Sweden)

    Ahmad F

    2012-04-01

    Full Text Available Paeonia officinalis (European peony, Common peony has been cultivated in Europe for years. The root has been used medicinally for over 2,000 years mainly in the treatment for epilepsy and to promote menstruation. Root is also antispasmodic, diuretic, sedative and tonic and has been successfully employed in the treatment of convulsions and spasmodic nervous affections such as epilepsy. It has also been used in the treatment of whooping cough whilst suppositories are sometimes made of the root to relieve anal and intestinal spasms, hemorrhoids and varicose veins. Experimentally it has been proved to have antihypertensive, abortifacient action and anti-ulcer activity. The roots of this plant are of great medicinal significance in unani system and homeopathy. The roots contain asparagin, benzoic acid, flavonoids, paeoniflorin, paeonin, paeonol, protoanemonin, tannic acid, triterpenoids, and volatile oil. This review covers botany, traditional uses and the phytoconstituents of the roots of Paeonia officinalis.

  1. MEDICINAL USES AND PHYTOCONSTITUENTS OF PAEONIA OFFICINALIS

    OpenAIRE

    Ahmad F; Tabassum N; Rasool S

    2012-01-01

    Paeonia officinalis (European peony, Common peony) has been cultivated in Europe for years. The root has been used medicinally for over 2,000 years mainly in the treatment for epilepsy and to promote menstruation. Root is also antispasmodic, diuretic, sedative and tonic and has been successfully employed in the treatment of convulsions and spasmodic nervous affections such as epilepsy. It has also been used in the treatment of whooping cough whilst suppositories are sometimes made of the root...

  2. Two new acetylenic compounds from Asparagus officinalis.

    Science.gov (United States)

    Li, Xue-Mei; Cai, Jin-Long; Wang, Wen-Xiang; Ai, Hong-Lian; Mao, Zi-Chao

    2016-01-01

    Two new acetylenic compounds, asparoffins A (1) and B (2), together with two known compounds, nyasol (3) and 3″-methoxynyasol (4), were isolated from stems of Asparagus officinalis. The structures of two new compounds were elucidated on the basis of detailed spectroscopic analyses (UV, IR, MS, 1D, and 2D NMR). All compounds were evaluated for their cytotoxicities against three human cancer cell lines.

  3. Two new acetylenic compounds from Asparagus officinalis.

    Science.gov (United States)

    Li, Xue-Mei; Cai, Jin-Long; Wang, Wen-Xiang; Ai, Hong-Lian; Mao, Zi-Chao

    2016-01-01

    Two new acetylenic compounds, asparoffins A (1) and B (2), together with two known compounds, nyasol (3) and 3″-methoxynyasol (4), were isolated from stems of Asparagus officinalis. The structures of two new compounds were elucidated on the basis of detailed spectroscopic analyses (UV, IR, MS, 1D, and 2D NMR). All compounds were evaluated for their cytotoxicities against three human cancer cell lines. PMID:26558641

  4. Development of mouthwash with Rosmarinus officinalis extract

    OpenAIRE

    Isabela Moreira Baumgratz de Paula; Flávia Costa Moraes; Orlando Vieira de Souza; Célia Hitomi Yamamoto

    2014-01-01

    Rosmarinus officinalis, which belongs to the Lamiaceaefamily, is a species of medicinal flora with therapeutic properties. In order to exploit the benefits of these properties, a mouthwash formulation was developed, with careful selection of raw materials to meet pharmacotechnical requirements. Extracts of the plant were incorporated into a mouthwash, which was shown to have inhibitory action in vitro against the micro-organisms commonly found in periodontics. Controls for assessing the quali...

  5. Antagonism of LIN-17/Frizzled and LIN-18/Ryk in nematode vulva induction reveals evolutionary alterations in core developmental pathways.

    Directory of Open Access Journals (Sweden)

    Xiaoyue Wang

    2011-07-01

    Full Text Available Most diversity in animals and plants results from the modification of already existing structures. Many organ systems, for example, are permanently modified during evolution to create developmental and morphological diversity, but little is known about the evolution of the underlying developmental mechanisms. The theory of developmental systems drift proposes that the development of conserved morphological structures can involve large-scale modifications in their regulatory mechanisms. We test this hypothesis by comparing vulva induction in two genetically tractable nematodes, Caenorhabditis elegans and Pristionchus pacificus. Previous work indicated that the vulva is induced by epidermal growth factor (EGF/RAS and WNT signaling in Caenorhabditis and Pristionchus, respectively. Here, we show that the evolution of vulva induction involves major molecular alterations and that this shift of signaling pathways involves a novel wiring of WNT signaling and the acquisition of novel domains in otherwise conserved receptors in Pristionchus vulva induction. First, Ppa-LIN-17/Frizzled acts as an antagonist of WNT signaling and suppresses the ligand Ppa-EGL-20 by ligand sequestration. Second, Ppa-LIN-18/Ryk transmits WNT signaling and requires inhibitory SH3 domain binding motifs, unknown from Cel-LIN-18/Ryk. Third, Ppa-LIN-18/Ryk signaling involves Axin and β-catenin and Ppa-axl-1/Axin is epistatic to Ppa-lin-18/Ryk. These results confirm developmental system drift as an important theory for the evolution of organ systems and they highlight the significance of protein modularity in signal transduction and the dynamics of signaling networks.

  6. Evaluation of analgesic activity of Emblica officinalis in albino rats

    Directory of Open Access Journals (Sweden)

    Bhomik Goel

    2014-04-01

    Results: Emblica officinalis extract did not produced statistically significant (p>0.05 analgesia when compared with the control group in hot plate latency, but produced a statistically significant reduction in 6% NaCl induced abdominal writhing (pEmblica officinalis exhibit analgesic activity involving peripheral mechanisms. [Int J Basic Clin Pharmacol 2014; 3(2.000: 365-368

  7. [Experimental studies on growing seedlings of Morinda officinalis How].

    Science.gov (United States)

    Wei, X; Pang, F; He, M; Hu, T

    1992-10-01

    By using the techniques of growing seedlings in bowshaped shed covered with plastic sheeting plus treatment with plant growth regulator, vegetative and generative propagation tests have been made of Morinda officinalis. Scientific evidences have thus been provided for growing seedlings of Morinda officinalis. PMID:1294174

  8. lin-12 Notch functions in the adult nervous system of C. elegans

    Directory of Open Access Journals (Sweden)

    Tucey Tim M

    2005-07-01

    Full Text Available Abstract Background Notch signaling pathways are conserved across species and traditionally have been implicated in cell fate determination during embryonic development. Notch signaling components are also expressed postdevelopmentally in the brains of adult mice and Drosophila. Recent studies suggest that Notch signaling may play a role in the physiological, rather than developmental, regulation of neurons. Here, we investigate a new non-developmental role for Caenorhabditis elegans lin-12 Notch signaling in neurons regulating the spontaneous reversal rate during locomotion. Results The spontaneous reversal rate of C. elegans during normal locomotion is constant. Both lin-12 gain and loss of function mutant animals had significantly increased reversal rates compared to wild type controls. These defects were caused by lin-12 activity, because the loss of function defect could be rescued by a wild type lin-12 transgene. Furthermore, overexpression of lin-12 recapitulated the gain-of-function defect. Increasing or decreasing lin-12 activity in the postdevelopmental adult animal was sufficient to rapidly and reversibly increase reversals, thereby excluding a developmental role for lin-12. Although lin-12 is expressed in the vulval and somatic gonad lineages, we find that these tissues play no role in regulating reversal rates. In contrast, altering lin-12 activity specifically in the nervous system was sufficient to increase reversals. These behavioral changes require components of the canonical lin-12 signaling cascade, including the ligand lag-2 and the transcriptional effector lag-1. Finally, the C. elegans AMPA/kainate glutamate receptor homolog glr-1 shows strong genetic interactions with lin-12, suggesting that glr-1 and/or other glutamate gated channels may be targets of lin-12 regulation. Conclusion Our results demonstrate a neuronal role for lin-12 Notch in C. elegans and suggest that lin-12 acutely regulates neuronal physiology to

  9. An Envoy of Friendship and Culture——In Celebration of Comrade Lin Lin's Ninety-fourth Birthday

    Institute of Scientific and Technical Information of China (English)

    GuZixin

    2004-01-01

    On September 27 this year,Comrade Lin Lin has reached the advanced age of ninety-four years. Besides being a senior on the diplomatic and cultural front of our country,Com. Lin Lin has long assumed leading position in our Association as well. In order to introduce his life and celebrate his birthday,

  10. A Dynamic Attitude Measurement System Based on LINS

    Directory of Open Access Journals (Sweden)

    Hanzhou Li

    2014-08-01

    Full Text Available A dynamic attitude measurement system (DAMS is developed based on a laser inertial navigation system (LINS. Three factors of the dynamic attitude measurement error using LINS are analyzed: dynamic error, time synchronization and phase lag. An optimal coning errors compensation algorithm is used to reduce coning errors, and two-axis wobbling verification experiments are presented in the paper. The tests indicate that the attitude accuracy is improved 2-fold by the algorithm. In order to decrease coning errors further, the attitude updating frequency is improved from 200 Hz to 2000 Hz. At the same time, a novel finite impulse response (FIR filter with three notches is designed to filter the dither frequency of the ring laser gyro (RLG. The comparison tests suggest that the new filter is five times more effective than the old one. The paper indicates that phase-frequency characteristics of FIR filter and first-order holder of navigation computer constitute the main sources of phase lag in LINS. A formula to calculate the LINS attitude phase lag is introduced in the paper. The expressions of dynamic attitude errors induced by phase lag are derived. The paper proposes a novel synchronization mechanism that is able to simultaneously solve the problems of dynamic test synchronization and phase compensation. A single-axis turntable and a laser interferometer are applied to verify the synchronization mechanism. The experiments results show that the theoretically calculated values of phase lag and attitude error induced by phase lag can both match perfectly with testing data. The block diagram of DAMS and physical photos are presented in the paper. The final experiments demonstrate that the real-time attitude measurement accuracy of DAMS can reach up to 20″ (1σ and the synchronization error is less than 0.2 ms on the condition of three axes wobbling for 10 min.

  11. Translator and Identity—Taking Lin Yutang as an Example

    Institute of Scientific and Technical Information of China (English)

    任春燕

    2016-01-01

    with the prosperous development of translation, the identity of translator has attracted more and more attention. The relationship of translator and identity is worth studying. This article takes translator Lin Yutang as an example to see how identities influence a translator. The conclusion is that the choice of translation martial, translation strategy, translation attitude and other aspects in translation are influenced by Li Yutang's identities such as his religious identity, class identity, national identity and personal identity.

  12. Detection of lin-4 mRNA in Caenorhabditis elegans Wild Type and Mutants via in Situ Hybridization%原位杂交检测lin-4 mRNA在Caenorhabditis elegans中的表达

    Institute of Scientific and Technical Information of China (English)

    高雅君; 杨玉荣

    2007-01-01

    lin-4是控制Caenorhabditis elegans(C.elegans)幼虫发育的异时性基因,也是一种小RNA分子(microRNA).通过整体原位杂交检测小RNA lin-4在野生型和lin-14、lin-28突变体中的区域性表达,探讨lin-4在C.elegans发育时空控制中的作用.结果表明:lin-4 mRNA在胚胎发育的早期和中期表达,胚胎后期至L1期末没有表达,之后又持续表达,成虫中也可以检测到lin-4 mRNA的存在.在lin-14和lin-28突变体中,lin-4的表达基本与野生型一致,不受lin-14、lin-28基因突变的影响,说明lin-14和lin-28是lin-4的下游基因.

  13. Lin-28 promotes symmetric stem cell division and drives adaptive growth in the adult Drosophila intestine.

    Science.gov (United States)

    Chen, Ching-Huan; Luhur, Arthur; Sokol, Nicholas

    2015-10-15

    Stem cells switch between asymmetric and symmetric division to expand in number as tissues grow during development and in response to environmental changes. The stem cell intrinsic proteins controlling this switch are largely unknown, but one candidate is the Lin-28 pluripotency factor. A conserved RNA-binding protein that is downregulated in most animals as they develop from embryos to adults, Lin-28 persists in populations of adult stem cells. Its function in these cells has not been previously characterized. Here, we report that Lin-28 is highly enriched in adult intestinal stem cells in the Drosophila intestine. lin-28 null mutants are homozygous viable but display defects in this population of cells, which fail to undergo a characteristic food-triggered expansion in number and have reduced rates of symmetric division as well as reduced insulin signaling. Immunoprecipitation of Lin-28-bound mRNAs identified Insulin-like Receptor (InR), forced expression of which completely rescues lin-28-associated defects in intestinal stem cell number and division pattern. Furthermore, this stem cell activity of lin-28 is independent of one well-known lin-28 target, the microRNA let-7, which has limited expression in the intestinal epithelium. These results identify Lin-28 as a stem cell intrinsic factor that boosts insulin signaling in intestinal progenitor cells and promotes their symmetric division in response to nutrients, defining a mechanism through which Lin-28 controls the adult stem cell division patterns that underlie tissue homeostasis and regeneration.

  14. Two New Iridoids from Verbena officinalis L.

    Directory of Open Access Journals (Sweden)

    Jicheng Shu

    2014-07-01

    Full Text Available Two new iridoids, 3-(5-(methoxycarbonyl-2-oxo-2H-pyran-3-ylbutanoic acid, named verbeofflin I (1, and 7-hydroxydehydrohastatoside (2, were isolated from the aerial part of Verbena officinalis L, along with three known iridoids, verbenalin (3, 3,4-dihydroverbenalin (4, hastatoside (5 by means of various column chromatography steps. The structures of these compounds were elucidated through analysis of their spectroscopic data obtained using 1D and 2D NMR and MS techniques. Verbeofflin I (1 is the new class of secoiridoid in the family Verbenaceae.

  15. The Impact of Aloe vera and Calendula on Perineal Healing after Episiotomy in Primiparous Women: A Randomized Clinical Trial

    Directory of Open Access Journals (Sweden)

    Farideh Eghdampour

    2013-11-01

    Full Text Available Introduction: Episiotomy is used for enlarging the perineum. Aloe vera and Calendula have been used for treating different diseases from ancient times, limited researches have been done regarding the healing of these plants. Since the effect of their ointment on episiotomy healing has not been studied, this study is being done for determining the impact of Aloe vera and Calendula on episiotomy healing in primiparous women. Methods: This clinical trial involves 111 qualified primiparous women admitted in Lolagar hospital. They were randomly categorized into three groups of control (n=1 and experimental (n=2 groups. The women in experimental group used Aloe vera and Calendula Ointment every 8 hours and the control group used hospital routine on episiotomy for 5 days. The data were collected by demographic questionnaire and redness, edema, ecchymosis, discharge and approximation scale (REEDA which investigated the episiotomy healing before and five days after intervention in two groups. ANOVA, Tukey test, Kruskal-wallis, Chi-square were used for data analysis. Results: The three groups do not have statistically significant different regarding demographic and other intervening variables. Comparing the mean of REEDA in five days after delivery showed statistically significant difference between control and experimental groups.Conclusion: According to the results, using Aloe vera and Calendula ointment considerably increases the speed of episiotomy wound healing so it can be used for quickening the episiotomy healing.

  16. Differences in crystallization of two LinB variants from Sphingobium japonicum UT26

    International Nuclear Information System (INIS)

    To study enzyme functionality, two haloalkane dehalogenase variants LinB32 and LinB70 carrying single-point and double-point mutations were constructed and crystallized in different crystallization conditions. Both LinB variants and their complexes with halogenated substrates diffracted to resolutions ranging from 1.6 to 2.8 Å. Haloalkane dehalogenases are microbial enzymes that convert a broad range of halogenated aliphatic compounds to their corresponding alcohols by the hydrolytic mechanism. These enzymes play an important role in the biodegradation of various environmental pollutants. Haloalkane dehalogenase LinB isolated from a soil bacterium Sphingobium japonicum UT26 has a relatively broad substrate specificity and can be applied in bioremediation and biosensing of environmental pollutants. The LinB variants presented here, LinB32 and LinB70, were constructed with the goal of studying the effect of mutations on enzyme functionality. In the case of LinB32 (L117W), the introduced mutation leads to blocking of the main tunnel connecting the deeply buried active site with the surrounding solvent. The other variant, LinB70 (L44I, H107Q), has the second halide-binding site in a position analogous to that in the related haloalkane dehalogenase DbeA from Bradyrhizobium elkanii USDA94. Both LinB variants were successfully crystallized and full data sets were collected for native enzymes as well as their complexes with the substrates 1,2-dibromoethane (LinB32) and 1-bromobutane (LinB70) to resolutions ranging from 1.6 to 2.8 Å. The two mutants crystallize differently from each other, which suggests that the mutations, although deep inside the molecule, can still affect the protein crystallizability

  17. lin-28 controls the succession of cell fate choices via two distinct activities.

    Directory of Open Access Journals (Sweden)

    Bhaskar Vadla

    Full Text Available lin-28 is a conserved regulator of cell fate succession in animals. In Caenorhabditis elegans, it is a component of the heterochronic gene pathway that governs larval developmental timing, while its vertebrate homologs promote pluripotency and control differentiation in diverse tissues. The RNA binding protein encoded by lin-28 can directly inhibit let-7 microRNA processing by a novel mechanism that is conserved from worms to humans. We found that C. elegans LIN-28 protein can interact with four distinct let-7 family pre-microRNAs, but in vivo inhibits the premature accumulation of only let-7. Surprisingly, however, lin-28 does not require let-7 or its relatives for its characteristic promotion of second larval stage cell fates. In other words, we find that the premature accumulation of mature let-7 does not account for lin-28's precocious phenotype. To explain let-7's role in lin-28 activity, we provide evidence that lin-28 acts in two steps: first, the let-7-independent positive regulation of hbl-1 through its 3'UTR to control L2 stage-specific cell fates; and second, a let-7-dependent step that controls subsequent fates via repression of lin-41. Our evidence also indicates that let-7 functions one stage earlier in C. elegans development than previously thought. Importantly, lin-28's two-step mechanism resembles that of the heterochronic gene lin-14, and the overlap of their activities suggests a clockwork mechanism for developmental timing. Furthermore, this model explains the previous observation that mammalian Lin28 has two genetically separable activities. Thus, lin-28's two-step mechanism may be an essential feature of its evolutionarily conserved role in cell fate succession.

  18. Study of calendula and Gaillardia growth in two composts prepared from agroindustrial wastes.

    Science.gov (United States)

    Roudsari, O Nouri; Akbari, B

    2007-05-01

    Two composts prepared from agroindustrial wastes were assayed as substrates: C1 from brewing waste (yeast and malt) plus lemon tree pruning and C2 from the solid fraction of olive mill wastewater plus olive leaves. Sixteen substrates were prepared by combining each compost with Sphagnum peat or a Commercial Substrate (CS) in different proportions. The nutrients (N and K) provided by the composts, which acted as slow-release fertilizers, influenced especially the development of calendula, although the physical and physicochemical properties such as total pore space and Electrical Conductivity (EC) were also relevant. On the other hand, in the salt-sensitive Gaillardia hybrid, EC and chloride concentration were the main factors influencing growth. The best results were found in substrates prepared by mixing C1 at up to 75% with peat, or at up to 50% with CS, or by mixing C2 at up to 50% with peat or CS, for calendula. For salt-sensitive species such as Gaillardia, adequate substrates for plant development were found for C1 at up to 50% with peat or CS, but the use of C2 should be limited to 25% in mixtures with peat or CS. Therefore, composts of agroindustrial origin such as these can be used as an alternative to peat and CSs for growing ornamental plants, provided the mixture contains at least 25% peat or CS. PMID:19069954

  19. The C. elegans Chp/Wrch Ortholog CHW-1 Contributes to LIN-18/Ryk and LIN-17/Frizzled Signaling in Cell Polarity.

    Directory of Open Access Journals (Sweden)

    Ambrose R Kidd

    Full Text Available Wnt signaling controls various aspects of developmental and cell biology, as well as contributing to certain cancers. Expression of the human Rho family small GTPase Wrch/RhoU is regulated by Wnt signaling, and Wrch and its paralog Chp/RhoV are both implicated in oncogenic transformation and regulation of cytoskeletal dynamics. We performed developmental genetic analysis of the single Caenorhabditis elegans ortholog of Chp and Wrch, CHW-1. Using a transgenic assay of the distal tip cell migration, we found that wild-type CHW-1 is likely to be partially constitutively active and that we can alter ectopic CHW-1-dependent migration phenotypes with mutations predicted to increase or decrease intrinsic GTP hydrolysis rate. The vulval P7.p polarity decision balances multiple antagonistic Wnt signals, and also uses different types of Wnt signaling. Previously described cooperative Wnt receptors LIN-17/Frizzled and LIN-18/Ryk orient P7.p posteriorly, with LIN-17/Fz contributing approximately two-thirds of polarizing activity. CHW-1 deletion appears to equalize the contributions of these two receptors. We hypothesize that CHW-1 increases LIN-17/Fz activity at the expense of LIN-18/Ryk, thus making the contribution of these signals unequal. For P7.p to polarize correctly and form a proper vulva, LIN-17/Fz and LIN-18/Ryk antagonize other Wnt transmembrane systems VANG-1/VanGogh and CAM-1/Ror. Our genetic data suggest that LIN-17/Fz represses both VANG-1/VanGogh and CAM-1/Ror, while LIN-18/Ryk represses only VANG-1. These data expand our knowledge of a sophisticated signaling network to control P7.p polarity, and suggests that CHW-1 can alter ligand gradients or receptor priorities in the system.

  20. The C. elegans Chp/Wrch Ortholog CHW-1 Contributes to LIN-18/Ryk and LIN-17/Frizzled Signaling in Cell Polarity.

    Science.gov (United States)

    Kidd, Ambrose R; Muñiz-Medina, Vanessa; Der, Channing J; Cox, Adrienne D; Reiner, David J

    2015-01-01

    Wnt signaling controls various aspects of developmental and cell biology, as well as contributing to certain cancers. Expression of the human Rho family small GTPase Wrch/RhoU is regulated by Wnt signaling, and Wrch and its paralog Chp/RhoV are both implicated in oncogenic transformation and regulation of cytoskeletal dynamics. We performed developmental genetic analysis of the single Caenorhabditis elegans ortholog of Chp and Wrch, CHW-1. Using a transgenic assay of the distal tip cell migration, we found that wild-type CHW-1 is likely to be partially constitutively active and that we can alter ectopic CHW-1-dependent migration phenotypes with mutations predicted to increase or decrease intrinsic GTP hydrolysis rate. The vulval P7.p polarity decision balances multiple antagonistic Wnt signals, and also uses different types of Wnt signaling. Previously described cooperative Wnt receptors LIN-17/Frizzled and LIN-18/Ryk orient P7.p posteriorly, with LIN-17/Fz contributing approximately two-thirds of polarizing activity. CHW-1 deletion appears to equalize the contributions of these two receptors. We hypothesize that CHW-1 increases LIN-17/Fz activity at the expense of LIN-18/Ryk, thus making the contribution of these signals unequal. For P7.p to polarize correctly and form a proper vulva, LIN-17/Fz and LIN-18/Ryk antagonize other Wnt transmembrane systems VANG-1/VanGogh and CAM-1/Ror. Our genetic data suggest that LIN-17/Fz represses both VANG-1/VanGogh and CAM-1/Ror, while LIN-18/Ryk represses only VANG-1. These data expand our knowledge of a sophisticated signaling network to control P7.p polarity, and suggests that CHW-1 can alter ligand gradients or receptor priorities in the system. PMID:26208319

  1. Inhibiton of photodynamic haemolysis by Gratiola officinalis L. extract

    Science.gov (United States)

    Tkachenko, Natalie; Pravdin, Alexander; Terentyuk, George; Navolokin, Nikita; Kurchatova, Maria; Polukonova, Natalia

    2015-03-01

    On the model of photodynamic haemolysis, the membranoprotective properties of a plant origin antioxidant, Gratiola officinalis L. extract, have been studied based on its ability to inhibit photodamage of sensitized erythrocyte membranes. The effect of different concentrations of the antioxidant on the photodynamic hemolysis has been studied; and the influence of incubation time on the membranoprotective properties of Gratiola officinalis L. extract has also been revealed.

  2. In vitro and ex vivo antiangiogenic activity of Salvia officinalis.

    Science.gov (United States)

    Keshavarz, Maryam; Mostafaie, Ali; Mansouri, Kamran; Bidmeshkipour, Ali; Motlagh, Hamid Reza Mohammadi; Parvaneh, Shahram

    2010-10-01

    Angiogenesis is a key process in the promotion of cancer and its metastasis. Herein, the antiangiogenic activity of Salvia officinalis extract and its fractions was investigated. S. officinalis aerial parts were extracted with ethanol and its successive hexane, ethyl acetate, n-butanol and aqueous fractions were evaluated for their antiangiogenic activities using human umbilical vein endothelial cells (HUVEC) capillary tube formation and rat aorta models in a three-dimensional collagen matrix. Furthermore, antimigrative effects of the fractions were assessed using a wound healing model. The ethanol extract of S. officinalis (ESO) potently inhibited capillary tube formation in HUVEC and rat aorta models of angiogenesis, and its hexane fraction (HSO) exerted the highest inhibitory effect. In addition, the ethanol extract of S. officinalis and its hexane fraction showed a dose-dependent inhibitory activity on the migration of the endothelial cells in the wound healing model. Furthermore, ESO inhibited endothelial cell proliferation at 50-200 μg/mL in a dose-dependent manner. These findings indicated some new pharmacological activities of S. officinalis such as antiangiogenic in vitro and ex vivo, and antimigrative activity in vitro. Therefore, S. officinalis could be a candidate as a useful herb with therapeutic or preventive activity against angiogenesis related disorders.

  3. The Effects of Medicinal Plants of Melissa officinalis and Salvia officinalis on Primary Dysmenorrhea

    Directory of Open Access Journals (Sweden)

    R. Kalvandi

    2014-07-01

    Full Text Available Introduction & Objective: Primary dysmenorrhea is one of the common problems in women, especially in young women. Although dysmenorrhea is not life threatening , it can have ad-verse effect on quality of life and cause disability or ineffectiveness. With regard to the young society suffering from it, various treatments are offered for dysmenorrhea one of which is medicinal plants. This study investigated the effect of Melissa officinalis and Salvia officinalis on primary dysmenorrhea Materials & Methods: This study was a clinical trial study performed on 50 girls with dys-menorrheal taking Salvia officinalis as infusion for five days before menstruation and five days during the menstruation. The consumption of the plants was repeated in the next two periods in the same way. Severity of pain, blooding duration and dysmenorrhea duration were evaluated with VAS (Visual Analog Scale Questionnaire in their next two cycles. Data were analyzed by chi-square, t-test and WILCOXON statistical test. Results: The results of this study showed that after using the plants, pain severity and pain duration significantly reduced P<0.01so that pain severity decreased from 6.30 to 3.94 and 3.24, (on a scale of 1 to 10 after taking the plants in the first and second periods ,respectively. The maximum pain duration before the treatment was 1 to 6 h (28.6% and this number reduced to less than 1 h after using these medicinal plants (38.0%. Mean of blooding duration was 6.36 days before the treatment. After the first and second duration, this value reached 6.48 and 6.34 days, respectively. It was determined that taking oral seda-tives reduced from 56% to 26% and 22% after the first and second periods, respectively. Fi-nally, after the second period, 42% of the girls reported high rates of satisfaction because of using the medicinal plants. Conclusion: Generally, it was showed that Melissa officinalis and Salvia officinalis signifi-cantly reduced pain severity and pain

  4. Major anthocyanins from purple asparagus (Asparagus officinalis).

    Science.gov (United States)

    Sakaguchi, Yumi; Ozaki, Yukio; Miyajima, Ikuo; Yamaguchi, Masaatsu; Fukui, Yuko; Iwasa, Keiko; Motoki, Satoru; Suzuki, Takashi; Okubo, Hiroshi

    2008-05-01

    Two major anthocyanins (A1 and A2) were isolated from peels of the spears of Asparagus officinalis cv. Purple Passion. They were purified by column, paper and high-performance liquid chromatographic separations, and their structures were elucidated by high-resolution Fourier transform ion cyclotron resonance mass spectrometry (HR-FT-ICR MS), 1H, 13C and two-dimensional NMR spectroscopic analyses and either acid or alkaline hydrolysis, respectively. A1 was identified as cyanidin 3-[3''-(O-beta-d-glucopyranosyl)-6''-(O-alpha-l-rhamnopyranosyl)-O-beta-d-glucopyranoside], whereas A2 was cyanidin 3-rutinoside, which is widely distributed in higher plants. Oxygen radical absorbance capacity (ORAC) assays proved their high antioxidant activities. PMID:18406435

  5. [Comparative study on kidney tonifying and yang supporting effects of different processed products of Morinda officinalis].

    Science.gov (United States)

    Cui, N; Shi, Ji; Jia, Tian-Zhu

    2013-11-01

    To compare the pharmacological effects for tonifying kidney and supporting Yang of different processed products of Morinda officinalis. The oral administration of adenine was performed to establish the kidney-Yang deficiency mice model. Four different processed products, namely M. officinalis, morinda pulp, salt-steamed M. officinalis and licorice-processed M. officinalis, were administered, with Guifu Dihuang pills as the control drug, and mice signs, weight, testicular coefficient, seminal vesicle coefficient, serum testosterone, serum cortisol and serum creatinine as evaluation indexes. The results showed that all of the four processed products of M. officinalis could improve the symptoms of the kidney-Yang deficient mice. Among them, salt-steamed M. officinalis had the most significant effect, which was followed by licorice-processed M. officinalis, morinda pulp and M. officinalis. PMID:24558872

  6. Monoterpene synthases from common sage (Salvia officinalis)

    Energy Technology Data Exchange (ETDEWEB)

    Croteau, Rodney Bruce (Pullman, WA); Wise, Mitchell Lynn (Pullman, WA); Katahira, Eva Joy (Pullman, WA); Savage, Thomas Jonathan (Christchurch 5, NZ)

    1999-01-01

    cDNAs encoding (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase from common sage (Salvia officinalis) have been isolated and sequenced, and the corresponding amino acid sequences has been determined. Accordingly, isolated DNA sequences (SEQ ID No:1; SEQ ID No:3 and SEQ ID No:5) are provided which code for the expression of (+)-bornyl diphosphate synthase (SEQ ID No:2), 1,8-cineole synthase (SEQ ID No:4) and (+)-sabinene synthase SEQ ID No:6), respectively, from sage (Salvia officinalis). In other aspects, replicable recombinant cloning vehicles are provided which code for (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase, or for a base sequence sufficiently complementary to at least a portion of (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant monoterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase may be used to obtain expression or enhanced expression of (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase in plants in order to enhance the production of monoterpenoids, or may be otherwise employed for the regulation or expression of (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase, or the production of their products.

  7. Uso da CALENDULA OFFICINALIS na prevenção e tratamento de radiodermite em cabeça e pescoço

    OpenAIRE

    Schneider, Franciane

    2013-01-01

    Resumo: Um dos efeitos adversos mais comuns do tratamento radioterápico é a radiodermite/radiodermatite, o qual é definida como um conjunto de lesões cutâneas provocadas por uma exposição excessiva à radiação ionizante; é considerada uma queimadura complexa que ocorre das estruturas internas às externas, levando à desidratação da pele, ocasionando algumas vezes complicações graves. A graduação de toxicidade da pele do paciente varia em uma escala de 0 a 4, conforme critérios do Radiation Ther...

  8. Effect of humic acid on morph-physiological traits, nutrients uptake and postharvest vase life of pot marigold cut flower (Calendula officinalis cv. Crysantha in hydroponic system

    Directory of Open Access Journals (Sweden)

    N. Allahvirdizadeh

    2014-07-01

    Full Text Available Humic acid (HA with pseudo-hormonal activity has an important effect on nutrients uptake, yield and quality of crop products. Therefore, this experiment was conducted in soilless condition to evaluate the best HA concentration (0, 250, 500, 750 and 1000 MG/l of nutrition solution and its effects on morphological traits, nutrients uptake, and postharvest vase life of pot marigold. Based on the results, HA showed significant effects on physio-morphological traits of pot marigold. Accordingly, as compared with control plants, the leaf area and chlorophyll content (a, b and total as plant photosynthetic capacity, flower number and total phenol concentration were significantly affected by HA application. Also, as compared with control plants (without HA application, HA showed a significant effect on P and Ca uptake. Flower longevity, as an important postharvest quality index, was increased 20% higher than control plants, in 250 mg/L concentration of HA. The results of the current investigation revealed that application of HA with 250 and 500 mg/L concentrations in soilless culture of pot marigold ‘cv. Crysantha’ could be recommended.

  9. Studies on Fertilization Effect and Fertilizer Formula Screen for Calendula officinalis L.%盆栽金盏菊氮磷钾肥料配方的研究

    Institute of Scientific and Technical Information of China (English)

    蒋志平; 鲁剑巍; 李文西; 邹娟

    2008-01-01

    采用"3414"不完全正交回归设计,利用盆栽试验研究金盏菊氮磷钾肥施用效果,并筛选适宜的肥料配方.结果表明,氮肥和磷肥对金盏菊的营养生长和生殖生长均有较大影响,而钾肥的效果主要表现在生长后期,氮、磷间有明显的正交互作用.以金盏菊总花数、功能叶片数、株高、植株干样质量为指标进行统计分析,得出盆栽(每盆装基质2 kg)金盏菊氮(N)、磷(P2O5)、钾(K2O)的最佳施用量为0.4、0.2和0.3 g·kg-1.

  10. The flavonoid glycosides from Rwandan Calendula officinalis L.%卢旺达产金盏菊花的黄酮苷类成分

    Institute of Scientific and Technical Information of China (English)

    陈玥; 石萍萍; 李晓霞; 刘丽丽; 王涛; 张祎

    2014-01-01

    目的 对卢旺达产金盏菊花的化学成分进行研究,为进一步开发利用该植物资源提供依据.方法 采用大孔吸附树脂、正相硅胶、Sephadex LH-20、反相ODS等柱色谱以及制备型高效液相色谱法等手段进行分离纯化,并通过理化性质与核磁共振等波谱学数据相结合的方法鉴定化合物的结构.结果 从金盏菊花体积分数为70%的乙醇溶液提取物中分离鉴定了6个单体成分,分别为异鼠李素-3-O-芸香糖苷(isorhamnetin-3-O-rutinoside,1)、异鼠李素-3-O-新橙皮糖苷(isorhamnetin-3-O-neohespeidoside,2)、香蒲新苷(typhaneoside,3)、槲皮素-3-O-β-D-新橙皮糖苷(quercetin-3-O-β-D-neohespeidoside,4)、芦丁(rutin,5)、槲皮素-3-O-(2”,6”-二-O-α-L-鼠李糖基)-β-D-葡萄糖苷(quercetin-3-O-(2",6"-di-O-α-L-rhamnopyranosyl)-β-D-glucopyranoside,6).结论 首次对东非产金盏菊干燥花的化学成分进行研究,分离得到了6个黄酮类化合物.

  11. Protective effects of ψ taraxasterol 3-O-myristate and arnidiol 3-O-myristate isolated from Calendula officinalis on epithelial intestinal barrier.

    Science.gov (United States)

    Dall'Acqua, Stefano; Catanzaro, Daniela; Cocetta, Veronica; Igl, Nadine; Ragazzi, Eugenio; Giron, Maria Cecilia; Cecconello, Laura; Montopoli, Monica

    2016-03-01

    The triterpene esters ᴪ taraxasterol-3-O-myristate (1) and arnidiol-3-O-myristate (2) were tested for their ability to protect epithelial intestinal barrier in an in vitro model. Their effects on ROS production and on trans-epithelial resistance were investigated on CaCo-2 cell monolayers both in basal and stress-induced conditions. Both compounds were able to modulate the stress damage induced by H2O2 and INFγ+TNFα, showing a potential use as model compounds for the study of new therapeutic agents for intestinal inflammations. PMID:26791917

  12. 金盏菊花黄色素的性质研究%Property of yellow pigment from Calendula officinalis L

    Institute of Scientific and Technical Information of China (English)

    胡晓丹; 谢笔钧; 王建中

    2001-01-01

    该文采用溶剂提取法和紫外可见分光光度法,研究了金盏菊花黄色素的提取方法、稳定性和抗氧化性以及色素提取物的各项指标.结果表明:金盏菊花黄色素主要是类胡萝卜素,用等体积的石油醚和丙酮作浸提剂有较高的提取率,所得提取物呈红色油状液体,具有油溶性,各项指标均符合国家标准;该色素对热、酸、碱、氧化剂、还原剂、室内散射光、紫外光、络合剂及大部分金属离子稳定,但对日光及少数金属离子(如Fe3+,Al3+,Cu2+)不稳定;该色素有显著的抗油脂氧化效果.

  13. Study on the morphology and histology of Calendula officinalis%金盏菊的形态组织学研究

    Institute of Scientific and Technical Information of China (English)

    郑佳; 卢先明; 高必兴; 辛天怡

    2014-01-01

    目的 研究金盏菊的形态组织学特征.方法 采用生药学常规方法对金盏菊进行性状鉴别、显微鉴别.结果 确定了金盏菊的药材性状、组织构造及粉末的主要鉴别特征.结论 组织学特征可作为金盏菊的鉴别依据.

  14. Uso da adubação orgânica e cobertura morta na cultura da calêndula (Calendula officinalis L.).

    OpenAIRE

    C.B.O. Araújo; Santos, A. M.; L. A. Fernandes; Martins, E. R.; R.A. Sampaio; Costa, C. A.; G.L.D. Leite

    2009-01-01

    A calêndula representa fonte alternativa econômica principalmente para a agricultura familiar. O objetivo do presente trabalho foi avaliar os efeitos da adubação orgânica e do uso da cobertura morta sobre a produção de biomassa e teor de flavonóides totais em plantas de calêndula. O experimento foi realizado no Campus de Montes Claros da Universidade Federal de Minas Gerais, utilizando-se o delineamento em blocos casualizados em esquema fatorial 2x2x4 com quatro repetições: duas épocas de col...

  15. Importance of the pluripotency factor LIN28 in the mammalian nucleolus during early embryonic development.

    Science.gov (United States)

    Vogt, Edgar J; Meglicki, Maciej; Hartung, Kristina Ilka; Borsuk, Ewa; Behr, Rüdiger

    2012-12-01

    The maternal nucleolus is required for proper activation of the embryonic genome (EGA) and early embryonic development. Nucleologenesis is characterized by the transformation of a nucleolar precursor body (NPB) to a mature nucleolus during preimplantation development. However, the function of NPBs and the involved molecular factors are unknown. We uncover a novel role for the pluripotency factor LIN28, the biological significance of which was previously demonstrated in the reprogramming of human somatic cells to induced pluripotent stem (iPS) cells. Here, we show that LIN28 accumulates at the NPB and the mature nucleolus in mouse preimplantation embryos and embryonic stem cells (ESCs), where it colocalizes with the nucleolar marker B23 (nucleophosmin 1). LIN28 has nucleolar localization in non-human primate (NHP) preimplantation embryos, but is cytoplasmic in NHP ESCs. Lin28 transcripts show a striking decline before mouse EGA, whereas LIN28 protein localizes to NPBs at the time of EGA. Following knockdown with a Lin28 morpholino, the majority of embryos arrest between the 2- and 4-cell stages and never develop to morula or blastocyst. Lin28 morpholino-injected embryos arrested at the 2-cell stage were not enriched with nucleophosmin at presumptive NPB sites, indicating that functional NPBs were not assembled. Based on these results, we propose that LIN28 is an essential factor of nucleologenesis during early embryonic development.

  16. CiLin Expansion and Its Application to Word Sense Disambiguation

    Institute of Scientific and Technical Information of China (English)

    Yao Jianmin(姚建民); Zhang Jing; Zhao Tiejun; Li Sheng

    2004-01-01

    40 000 new entries are added to CiLin in knowledge engineering. Statistics are presented about the sense ambiguity of Chinese words in CiLin. An automatic solution is proposed for the problem of Chinese sense disambiguation, which utilizes the heuristics of part-of-speech tagging and Nave Bayes classification based on a partially disambiguated corpus.

  17. Fetal deficiency of lin28 programs life-long aberrations in growth and glucose metabolism.

    Science.gov (United States)

    Shinoda, Gen; Shyh-Chang, Ng; Soysa, T Yvanka de; Zhu, Hao; Seligson, Marc T; Shah, Samar P; Abo-Sido, Nora; Yabuuchi, Akiko; Hagan, John P; Gregory, Richard I; Asara, John M; Cantley, Lewis C; Moss, Eric G; Daley, George Q

    2013-08-01

    LIN28A/B are RNA binding proteins implicated by genetic association studies in human growth and glucose metabolism. Mice with ectopic over-expression of Lin28a have shown related phenotypes. Here, we describe the first comprehensive analysis of the physiologic consequences of Lin28a and Lin28b deficiency in knockout (KO) mice. Lin28a/b-deficiency led to dwarfism starting at different ages, and compound gene deletions showed a cumulative dosage effect on organismal growth. Conditional gene deletion at specific developmental stages revealed that fetal but neither neonatal nor adult deficiency resulted in growth defects and aberrations in glucose metabolism. Tissue-specific KO mice implicated skeletal muscle-deficiency in the abnormal programming of adult growth and metabolism. The effects of Lin28b KO could be rescued by Tsc1 haplo-insufficiency in skeletal muscles. Our data implicate fetal expression of Lin28a/b in the regulation of life-long effects on metabolism and growth, and demonstrate that fetal Lin28b acts at least in part via mTORC1 signaling.

  18. Lin James 《Basically, we want to be global from China》

    Institute of Scientific and Technical Information of China (English)

    HAYET SELLAMI

    2006-01-01

    @@ James Lin's travel to Beijing was hasty. His secretary could barely find a time for an interview, finally managing to squeeze an hour meeting with Lin at his hotel just after breakfast and before his appointments with his Beijing staff, officials and various local media.

  19. Coarse-graining the Lin-Maldacena geometries

    International Nuclear Information System (INIS)

    The Lin-Maldacena geometries are nonsingular gravity duals to degenerate vacuum states of a family of field theories with SU(2|4) supersymmetry. In this note, we show that at large N, where the number of vacuum states is large, there is a natural 'macroscopic' description of typical states, giving rise to a set of coarse-grained geometries. For a given coarse-grained state, we can associate an entropy related to the number of underlying microstates. We find a simple formula for this entropy in terms of the data that specify the geometry. We see that this entropy function is zero for the original microstate geometries and maximized for a certain 'typical state' geometry, which we argue is the gravity dual to the zero-temperature limit of the thermal state of the corresponding field theory. Finally, we note that the coarse-grained geometries are singular if and only if the entropy function is non-zero

  20. Interaction dynamique non-linéaire sol-structure

    OpenAIRE

    Saez Robert, Esteban

    2009-01-01

    L’interaction dynamique entre le sol et les structures (IDSS) a fait l’objet de nombreuses études sous l’hypothèse de l’élasticité linéaire, bien que les effets de l’IDSS puissent être différents entre un système élastique et un système inélastique. De fait, les méthodologies usuelles développées à partir des études élastiques peuvent ne pas être adaptées aux bâtiments conçus pour dissiper de l’énergie par de l’endommagement lors de séismes sévères. De plus, il est bien connu que la limite d’...

  1. Rosmarinus officinalis essential oil: antiproliferative, antioxidant and antibacterial activities

    Directory of Open Access Journals (Sweden)

    Abdullah Ijaz Hussain

    2010-12-01

    Full Text Available The aim of this work was to investigate and compare the antiproliferative, antioxidant and antibacterial activities of Rosmarinus officinalis essential oil, native to Pakistan. The essential oil content from the leaves of R. officinalis was 0.93 g 100g-1. The GC and GC-MS analysis revealed that the major components determined in R. officinalis essential oil were 1,8-cineol (38.5%, camphor (17.1%, α-pinene (12.3%, limonene (6.23%, camphene (6.00% and linalool (5.70%. The antiproliferative activity was tested against two cancer (MCF-7 and LNCaP and one fibroblast cell line (NIH-3T3 using the MTT assay, while, the antioxidant activity was evaluated by the reduction of 2, 2-diphenyl-1-picryl hydrazyl (DPPH and measuring percent inhibition of peroxidation in linoleic acid system. The disc diffusion and modified resazurin microtitre-plate assays were used to evaluate the inhibition zones (IZ and minimum inhibitory concentration (MIC of R. officinalis essential oil, respectively. It is concluded from the results that Rosmarinus officinalis essential oil exhibited antiproliferative, antioxidant and antibacterial activities.

  2. Comparing the effects of Bentonite & Calendula on the improvement of infantile diaper dermatitis: A randomized controlled trial

    Science.gov (United States)

    Mahmoudi, Mansoreh; Adib-Hajbaghery, Mohsen; Mashaiekhi, Mahdi

    2015-01-01

    Background & objectives: Infantile diaper dermatitis is a common, acute inflammatory reaction of the skin around diaper among infants. This study was undertaken to compare the effect of topical application of Bentonite and Calendula creams on the improvement of infantile diaper dermatitis. Methods: This double blind randomized controlled trial was undertaken on 100 patients of infantile diaper dermatitis. The 100 participants were randomly assigned into two groups of 50 each, and were prescribed the coded medicine. The mothers were trained to apply the cream and level of improvement was judged by observing the affected area on the first visit and then after three days of receiving treatment. Results: The mean age of infants was 6.45±5.53 months in Calendula group and 7.35±6.28 months in Bentonite group. Overall, 88 per cent of lesions in the Bentonite group started improving in the first six hours while this rate was 54 per cent in Calendula group (Pdiaper dermatitis (IRCT ID: IRCT 2012112811593N1). PMID:26831423

  3. Bacterial diversity and real-time PCR based assessment of linA and linB gene distribution at hexachlorocyclohexane contaminated sites.

    Science.gov (United States)

    Lal, Devi; Jindal, Swati; Kumari, Hansi; Jit, Simran; Nigam, Aeshna; Sharma, Pooja; Kumari, Kirti; Lal, Rup

    2015-03-01

    The disposal of hexachlorocyclohexane (HCH) muck has created large number of HCH dumpsites all over the world from where the harmful HCH isomers are leaking into the environment. Bacteria have evolved at such contaminated sites that have the ability to degrade HCH. Degradation of various HCH isomers in bacterial strains is mediated primarily by two genes: linA and linB which encode dehydrochlorinase and haloalkane dehalogenase respectively. In this study we explored one such highly contaminated HCH dumpsite located in Lucknow, Uttar Pradesh, India. To assess the biostimulation potential of the contaminated site, microbial diversity study and real-time PCR based quantification of lin genes was carried out. The soil samples from dumpsite and surrounding areas were found to be highly contaminated with HCH residue levels as high as 1.8 × 10(5)  mg kg(-1). The residues were detected in areas upto 13 km from the dumpsite. Sphingomonads, Chromohalobacter, and Marinobacter were the dominant genera present at the dump-site. Role of Sphingomonads in HCH degradation has been well documented. The highest copy numbers of linA and linB genes as determined using real-time PCR were 6.2 × 10(4) and 5.3 × 10(5), respectively, were found in sample from the dump site. The presence of Sphingomonads, linA, and linB genes from HCH contaminated soil indicates the presence of indigenous bacterial communities capable of HCH degradation.

  4. The C. elegans hox gene lin-39 controls cell cycle progression during vulval development.

    Science.gov (United States)

    Roiz, Daniel; Escobar-Restrepo, Juan Miguel; Leu, Philipp; Hajnal, Alex

    2016-10-01

    Cell fate specification during organogenesis is usually followed by a phase of cell proliferation to produce the required number of differentiated cells. The Caenorhabditis elegans vulva is an excellent model to study how cell fate specification and cell proliferation are coordinated. The six vulval precursor cells (VPCs) are born at the first larval stage, but they arrest in the G1 phase of the cell cycle until the beginning of the third larval stage, when their fates are specified and the three proximal VPCs proliferate to generate 22 vulval cells. An epidermal growth factor (EGF) signal from the gonadal anchor cell combined with lateral DELTA/NOTCH signaling between the VPCs determine the primary (1°) and secondary (2°) fates, respectively. The hox gene lin-39 plays a key role in integrating these spatial patterning signals and in maintaining the VPCs as polarized epithelial cells. Using a fusion-defective eff-1(lf) mutation to keep the VPCs polarized, we find that VPCs lacking lin-39 can neither activate lateral NOTCH signaling nor proliferate. LIN-39 promotes cell cycle progression through two distinct mechanisms. First, LIN-39 maintains the VPCs competent to proliferate by inducing cdk-4 cdk and cye-1 cyclinE expression via a non-canonical HOX binding motif. Second, LIN-39 activates in the adjacent VPCs the NOTCH signaling pathway, which promotes VPC proliferation independently of LIN-39. The hox gene lin-39 is therefore a central node in a regulatory network coordinating VPC differentiation and proliferation.

  5. Supercritical carbon dioxide extraction of antioxidants from rosemary (Rosmarinus officinalis L. and sage (Salvia officinalis L.

    Directory of Open Access Journals (Sweden)

    JASNA IVANOVIĆ

    2009-07-01

    Full Text Available The aim of the present study was to isolate and characterize antioxidant extracts obtained from dried leaves of rosemary (Rosmarinus officinalis L. and sage (Salvia officinalis L., originating from the southern Balkan Region. The antioxidant fraction was isolated from the plant material by supercritical carbon dioxide (SC-CO2 fractional extraction under a pressure of 30 MPa and at temperatures of 40 and 100 °C. In the present study, kinetic data and yields of antioxidant extracts obtained from dried leaves of rosemary and sage under different conditions were determined. Electron spin resonance (ESR spectroscopy assay on the ability of the extracts to scavenge stable 2,2-diphenyl-1-picrylhydrazyl (DPPH free radicals and reactive hydroxyl radicals during the Fenton reaction trapped by 5,5-dimethyl-1-pyrroline-N-oxide (DMPO showed that the investigated extracts had antioxidant activity comparable to that of butylated hydroxyanisole (BHA and commercial rosemary extract. The antioxidant fractions isolated at the higher temperature had higher antioxidant activities. A tentative analysis of the chemical composition of the antioxidant fractions obtained at the higher temperature was accomplished by LC-DAD and LC-MS analytical methods. Abietane-type diterpenoids, flavonoids and fatty acids were identified in the SC-CO2 extract of rosemary and sage.

  6. Amtimicrobial activity of essential oil of Melissa officinalis L, Lamiaceae

    Directory of Open Access Journals (Sweden)

    Aničić Nada V.

    2005-01-01

    Full Text Available The antimicrobial activity of the essential oil of Melissa officinalis was investigated in this paper. The essential oil was obtained by the principle of water and steam and analyzed by GC and GC-MS using FID and MSD. The main components of the oil of Melissa officinalis were geranial (17.30%, neral (14.70% and citronellal (10.70%. The antimicrobial properties were tested against the following bacterial species: B subtilis, B.cereus Bifidobacterium sp., Corynobacterium sp., E. coli, Klebsiella sp., L. acidophilus, L. fermentum, L. plantarum, L. rhamnosus, Listeria monocytogenes, P. vulgaris, P. aerugenosa, S. enteritidis, Shigella sp., S. aureus, and fungi Candida albicans, Alternarija sp. and Aspergillus niger. The diffusion technique was used for testing: the antimicrobial activity, and the MIC was determined by the broth dilution method. The essential oil of M. officinalis showed high antimicrobial activity.

  7. Steroidal saponins from roots of Asparagus officinalis.

    Science.gov (United States)

    Huang, Xuefeng; Kong, Lingyi

    2006-02-01

    Sarsasapogenin M (1) and sarsasapogenin N (2), two new oligospirostanosides with a unique aglycone moiety, (25S)-5beta-spirostan-3beta, 17alpha-diol, along with seven known compounds (25S)-5beta-spirostan-3beta-ol-3-O-beta-d-glucopyranosyl-(1,2)-[beta-d-xylopyranosyl-(1,4)]-beta-d-glucopyranoside (3), (25S)-5beta-spirostan-3beta-ol-3-O-beta-d-glucopyranosyl-(1,2)-beta-d-glucopyranoside (4), (25S)-5beta-spirostan-3beta-ol-3-O-alpha-l-rhamnopyranosyl-(1,2)-[alpha-l-rhamnopyranosyl-(1,4)]-beta-d-glucopyranoside (5), (25S)26-O-beta-d-glucopyranosyl-5beta-furost-20 (22)-ene-3beta,26-diol-3-O-beta-d-glucopyranosyl-(1,2)-beta-d-glucopyranoside (6), yamogenin (7), beta-sitosterol (8), and sitosterol-beta-d-glucoside (9) were isolated from the roots of Asparagus officinalis L. Their structures were determined by spectral analysis, including extensive 1D and 2D NMR experiments. PMID:16280142

  8. Main design elements of the superconducting magnet for the LIN-5B baseball trap

    International Nuclear Information System (INIS)

    A design of superconducting magnet system (SMS) is described for the LIN-5B baseball trap elaborated for the OGRA-3B experimental installation. The general layout of the trap, cross sections of the superconducting coil and load-bearing frame, the current leads arrangement and main LIN-5B SMS parameters are presented. A three-year experience in operation of the baseball LIN-5B SMS and successful tests followed by achieving critical conditions four times permit to make the conclusion that the design and SMS assembly meet the requirements

  9. LIN-39/Hox triggers cell division and represses EFF-1/fusogen-dependent vulval cell fusion

    OpenAIRE

    Shemer, Gidi; Podbilewicz, Benjamin

    2002-01-01

    General mechanisms by which Hox genes establish cell fates are known. However, a few Hox effectors mediating cell behaviors have been identified. Here we found the first effector of LIN-39/HoxD4/Dfd in Caenorhabditis elegans. In specific vulval precursor cells (VPCs), LIN-39 represses early and late expression of EFF-1, a membrane protein essential for cell fusion. Repression of eff-1 is also achieved by the activity of CEH-20/Exd/Pbx, a known cofactor of Hox proteins. Unfused VPCs in lin-39(...

  10. Rosmarinic Acid and Melissa officinalis Extracts Differently Affect Glioblastoma Cells

    OpenAIRE

    Ramanauskiene, Kristina; Raudonis, Raimondas; Majiene, Daiva

    2016-01-01

    Lemon balm (Melissa officinalis L.) has many biological effects but especially important is its neuroprotective activity. The aim of the study is to produce different extracts of Melissa officinalis and analyse their chemical composition and biological properties on rat glioblastoma C6 cells. Results revealed that rosmarinic acid (RA) is the predominant compound of lemon balm extracts. RA has cytotoxic effect on glioblastoma cells (LC50 290.5 μM after the incubation of 24 h and LC50 171.3 μM ...

  11. Characteristics of LIN Bus Maintenance Technology%LIN总线维修技术特性

    Institute of Scientific and Technical Information of China (English)

    李倩

    2014-01-01

    Local interconnect network (LIN) is a kind of serial data communication network telecommunication line. The application of LIN greatly reduces the use number of vehicle electrical circuit, and decreases the electrical circuit fault rate. This paper mainly introduces the LIN bus and LIN bus diagnostic characteristics from the aspects of transmission mode, signal characteristics and so on.%局域互联网络(LIN)是一类串行数据通信的网络通信线,LIN的应用大大减少了车辆电气线路的使用数量,降低了电气线路的故障发生率。本文主要从传输方式、信号特性方面介绍LIN总线特性及LIN总线诊断特性。

  12. Constituintes das sementes de Copaifera officinalis L. Constituents from Copaifera officinalis L. seeds

    Directory of Open Access Journals (Sweden)

    Valdir F. Veiga Junior

    2007-01-01

    Full Text Available O gênero Copaifera L. é um dos mais importantes economicamente na Região Amazônica devido, principalmente, à produção dos óleos de copaíba, oléo-resinas com diversas propriedades farmacológicas confirmadas. Apesar disso, os estudos fitoquímicos com as sementes das árvores do gênero Copaifera L.são raros. Copaifera officinalis foi a primeira espécie do gênero Copaifera a ser descrita. Este trabalho descreve a composição dos extratos obtidos em hexano e em acetato de etila das sementes de C. officinalis. No extrato obtido em hexano, a análise por cromatografia em fase gasosa utilizando padrões e através de espectrometria de massas permitiu a identificação de: esqualeno, tetradecano, hexadecano, campesterol, estigmasterol e beta-sitosterol; os ácidos graxos hexadecanóico, 9-octadecenóico e octadecanóico (majoritários; e decanóico, eicosanóico, docosanóico e tetracosanóico (minoritários. Cumarina foi isolada do extrato em acetato de etila e identificada por técnicas de RMN.Copaifera L. is one of the most economically important plant genera in the Amazon Region, since it exudes a resin-oil named copaiba oil possessing several confirmed pharmacological properties. In spite of that, phytochemical studies of the seeds from this genus are rare. Copaifera officinalis L. was the first species in the genus Copaifera to be described. This paper describes the chemical composition of the hexane and ethyl acetate extracts of the seeds from this species. In the hexane extract, gas chromatography and mass spectrometry analyses enabled us to identify squalene, tetradecane, hexadecane, campesterol, stigmasterol and beta-sitosterol; hexadecanoic, 9-octadecenoic and octadecanoic acids (major substances; as well as decanoic, eicosanoic, docosanoic and tetracosanoic (minor substances acids. Coumarin was detected in the ethyl acetate extract, isolated and identified by NMR.

  13. LIN28 Is Involved in Glioma Carcinogenesis and Predicts Outcomes of Glioblastoma Multiforme Patients

    OpenAIRE

    Qin, Rong; ZHOU, JINGXU; Chen, Chao; Xu, Tao; Yan, Yong; Ma, Yushui; Zheng, Zongli; Shen, Yiping; Lu, Yicheng; Fu, Da; Chen, Juxiang

    2014-01-01

    LIN28, an evolutionarily conversed RNA binding protein which can bind to the terminal loops of let-7 family microRNA precursors and block their processing to maturation, is highly expressed in several subsets of tumors that carry poor prognoses, such as ovarian carcinoma, hepatocellular carcinoma, colon carcinoma and germ cell carcinoma. However, there has been no study on the expression of LIN28 in glioma tissues or their importance as a prognostic predictor of glioma patients. This study ai...

  14. LIN28 is involved in glioma carcinogenesis and predicts outcomes of glioblastoma multiforme patients.

    Directory of Open Access Journals (Sweden)

    Rong Qin

    Full Text Available LIN28, an evolutionarily conversed RNA binding protein which can bind to the terminal loops of let-7 family microRNA precursors and block their processing to maturation, is highly expressed in several subsets of tumors that carry poor prognoses, such as ovarian carcinoma, hepatocellular carcinoma, colon carcinoma and germ cell carcinoma. However, there has been no study on the expression of LIN28 in glioma tissues or their importance as a prognostic predictor of glioma patients. This study aimed to examine the expression of LIN28 in glioma and correlate the results to patient outcome. We found that LIN28 expression was significantly higher in the group of patients with a poor prognosis compared to patients with a good prognosis by gene microarray. Log-rank analysis showed patients with higher LIN28 expression level in tumor had a shorter progression-free survival and overall survival times compared to those with lower LIN28 expression level. Similar results were also obtained from the tissue microarray analysis. Univariate and multivariate analyses showed high LIN28 expression was an independent prognostic factor for a shorter progression-free survival and overall survival in GBM patients. Furthermore in vitro experiments showed that down-regulation of LIN28 in U251 and U373 cells caused cell cycle arrest in the G1 phase, delayed cell proliferation, increased apoptosis, and resulted in fewer colonies compared to controls. Summarily, our data provides a potential target for cancer therapy as an approach to overcome the poor options currently available for GBM patients.

  15. Hypersensitivity to Parietaria officinalis pollen in newcomers to the area with the plant.

    Science.gov (United States)

    Cvitanović, S; Marusić, M; Juricić, M; Vrdoljak, E; Petrovecki, M; Rozga, A; Stavljenić-Rukavina, A

    1993-11-01

    Hypersensitivity to Parietaria officinalis (wall pellitory) pollen and other environmental allergens was studied in pollinosis patients allergic to P. officinalis pollen who were born in areas without P. officinalis and later moved to the city of Split, where P. officinalis is responsible for some 65% of pollinosis cases. Highly significant positive correlations were found for both the intensity of skin test reaction and concentration of specific serum IgE with the length of residence in the area. In contrast, the respective data on subjects hypersensitive to P. officinalis pollen allergen, but born and living in the area of Split, revealed a tendency to negative correlation between age and intensity of hypersensitivity to P. officinalis. A number of patients from both groups were tested for presence of serum IgE antibodies specific for 14 common environmental allergens. Hypersensitivity to P. officinalis pollen was associated with hypersensitivity to olive, mugwort, and birch pollen in newcomers; hypersensitivity to birch and, to some extent, olive pollen was significantly more frequent in newcomers than in autochthonous patients who were allergic to P. officinalis pollen. Regardless of whether the patients were autochthons or newcomers to the area with P. officinalis, hypersensitivity to P. officinalis mostly excluded hypersensitivity to Dermatophagoides farinae and D. pteronyssinus, and vice versa.

  16. Ameliorative effects of thyme and calendula extracts alone or in combination against aflatoxins-induced oxidative stress and genotoxicity in rat liver

    OpenAIRE

    Abdel-Aziem, Sekena H.; Hassan, Aziza M.; El-Denshary, Ezzeldein S.; Hamzawy, Mohamed A.; Mannaa, Fathia A.; Mosaad A. Abdel-Wahhab

    2013-01-01

    The aims of the current work were to evaluate the hepatoprotective effect of calendula flowers and/or thyme leave extracts on aflatoxins (AFs)-induced oxidative stress, genotoxicity and alteration of p53 bax and bcl2 gene expressions. Eighty male Sprague–Dawley rats were divided into eight equal groups including: the control group, the group fed AFs-contaminated diet (2.5 mg/kg diet) for 5 weeks, the groups treated orally with thyme and/or calendula extract (0.5 g/kg b.w) for 6 weeks and the ...

  17. Structure and Mechanism of the Lincosamide Antibiotic adenylyltransferase LinB

    Energy Technology Data Exchange (ETDEWEB)

    Morar, M.; Buhllar, K; Hughes, D; Junop, M; Wright, G

    2009-01-01

    Lincosamides make up an important class of antibiotics used against a wide range of pathogens, including methicillin-resistant Staphylococcus aureus. Predictably, lincosamide-resistant microorganisms have emerged with antibiotic modification as one of their major resistance strategies. Inactivating enzymes LinB/A catalyze adenylylation of the drug; however, little is known about their mechanistic and structural properties. We determined two X-ray structures of LinB: ternary substrate and binary productbound complexes. Structural and kinetic characterization of LinB, mutagenesis, solvent isotope effect, and product inhibition studies are consistent with a mechanism involving direct in-line nucleotidyl transfer. The characterization of LinB enabled its classification as a member of a nucleotidyltransferase superfamily, along with nucleotide polymerases and aminoglycoside nucleotidyltransferases, and this relationship offers further support for the LinB mechanism. The LinB structure provides an evolutionary link to ancient nucleotide polymerases and suggests that, like protein kinases and acetyltransferases, these are proto-resistance elements from which drug resistance can evolve.

  18. Two New Triterpenoids from the Roots of Sanguisorba officinalis L.

    Institute of Scientific and Technical Information of China (English)

    Fan ZHANG; Tie-Jun FU; Shu-Lin PENG; Zhong-Rong LIU; Li-Sheng DING

    2005-01-01

    Two new triterpenoids, octanordammar- 1,11,13(17)-trien- 17-ol-3,16-dione (1) and lup- 12-en- 15α,19β-diol-3,11-dioxo-28-oic acid (4), as well as 13 known compounds were isolated from the roots of Sanguisorba officinalis L. (Rosaceae). Their structures were determined using spectroscopic methods.

  19. Methyl carnosate, an antibacterial diterpene isolated from Salvia officinalis leaves.

    Science.gov (United States)

    Climati, Elisa; Mastrogiovanni, Fabio; Valeri, Maria; Salvini, Laura; Bonechi, Claudia; Mamadalieva, Nilufar Zokirzhonovna; Egamberdieva, Dilfuza; Taddei, Anna Rita; Tiezzi, Antonio

    2013-04-01

    Ethanolic extracts of Salvia officinalis leaves demonstrated antibacterial activity against Bacillus cereus. Fractionation of the extracts led to the isolation of the most active antibacterial compound, which, from spectroscopic and LC-MS evidence, was proved to be the diterpene, methyl carnosate.

  20. Isolation and Purification Chemical Compound from Melissa officinalis

    Directory of Open Access Journals (Sweden)

    Jamal A Nasser

    2014-01-01

    Full Text Available A chemical study was done on the leaves of Melissa officinalis. The separations of the chemical components were carried out by different chromatographic techniques and their structures were elucidated by spectroscopic method including nuclear magnetic resonance as well as gas chromatography-mass spectrometry. Three compounds were isolated and identified as friedoolean-7-one , stigmasterol and betulinic acid.

  1. The influence of photoperiodicity on hatching of Sepia Officinalis

    NARCIS (Netherlands)

    Paulij, W.P.; Herman, P.M.J.; Roozen, M.E.F.; Denucé, J.M.

    1991-01-01

    The influence of photoperiodicity on hatching of Sepia officinalis was investigated under different experimental light-dark (LD) conditions. The results are viewed in relation to some relevant properties of the perivitelline fluid (PVF) and the egg capsule during embryonic development. In embryos of

  2. Identification of sex pheromone of calendula plume mothPlatyptilia williamsii.

    Science.gov (United States)

    Haynes, K F

    1987-04-01

    The sex pheromone of the calendula plume moth,Platyptilia williamsii was identified as (Z)-11-hexadecenal (Z11-16∶Aid). Extracts of female sex pheromone glands contained several compounds when analyzed by capillary and packed-column GLC. However, airborne collections of volatiles from glands contained only one of these compounds, having the same retention time asZ11-16∶Ald. GC-MS and microozonolysis analyses of the natural product were consistent with those of syntheticZ11-16∶Ald. In a flight tunnel, males oriented upwind and touched sources ofZ11-16∶Ald and gland extract with equal frequency. Field tests of syntheticZ11-16∶Ald already have shown it to be a potent sex attractant for males of this species. This study further supports the hypothesis thatP. williamsii and a sympatric species,Platyptilia carduidactyla, are not reproductively isolated by chemical differences in the composition of the sex pheromone, but rather by temporal differences in sexual activities. PMID:24302055

  3. Ameliorative effects of thyme and calendula extracts alone or in combination against aflatoxins-induced oxidative stress and genotoxicity in rat liver.

    Science.gov (United States)

    Abdel-Aziem, Sekena H; Hassan, Aziza M; El-Denshary, Ezzeldein S; Hamzawy, Mohamed A; Mannaa, Fathia A; Abdel-Wahhab, Mosaad A

    2014-05-01

    The aims of the current work were to evaluate the hepatoprotective effect of calendula flowers and/or thyme leave extracts on aflatoxins (AFs)-induced oxidative stress, genotoxicity and alteration of p53 bax and bcl2 gene expressions. Eighty male Sprague-Dawley rats were divided into eight equal groups including: the control group, the group fed AFs-contaminated diet (2.5 mg/kg diet) for 5 weeks, the groups treated orally with thyme and/or calendula extract (0.5 g/kg b.w) for 6 weeks and the groups pretreated orally with thyme and/or calendula extract 1 week before and during AFs treatment for further 5 weeks. Blood, liver and bone marrow samples were collected for biochemical analysis, gene expression, DNA fragmentation and micronucleus assay. The results showed that AFs induced significant alterations in oxidative stress markers, increased serum AFP and inflammatory cytokine, percentage of DNA fragmentation, the expression of pro-apoptotic gene p53 and bax accompanied with a decrease in the expression of bcl2. Animals treated with the extracts 1 week before AFs treatment showed a significant decrease in oxidative damage markers, micronucleated cells, DNA fragmentation and modulation of the expression of pro-apoptotic genes. These results suggested that both calendula and thyme extracts had anti-genotoxic effects due to their higher content of total phenolic compounds. PMID:24096837

  4. Chemical composition of Rosmarinus officinalis essential oil and antioxidant action against gastric damage induced by absolute ethanol in the rat

    Directory of Open Access Journals (Sweden)

    Christiane Takayama

    2016-08-01

    Conclusions: We suggest that the monoterpenes present in the essential oil obtained from R. officinalis may be among the active principles responsible for the antioxidant activity shown by essential oil of R. officinalis.

  5. νLIN6: An Integrated Mobility Protocol in IPv6

    Science.gov (United States)

    Banno, Ayumi; Teraoka, Fumio

    This paper proposes a protocol called νLIN6 which supports both network mobility and host mobility in IPv6. There are several proposals to support network mobility and host mobility. Network Mobility (NEMO) Basic Support Protocol has several problems such as pinball routing, large header overhead due to multiple levels of tunneling, and a single point of failure. Optimized NEMO (ONEMO) and Mobile IP with Address Translation (MAT) are solutions to provide route optimization, but they generate a lot of signaling messages at a handover. In νLIN6, packet relay is required only once regardless of the nested level in network mobility while optimal routing is always provided in host mobility. A fixedsized extension header is used in network mobility while there is no header overhead in host mobility. νLIN6 is more tolerant of network failure and mobility agent failure than NEMO Basic Support Protocol. It also allows ordinary IPv6 nodes to communicate with mobile nodes and nodes in the mobile network. We implemented νLIN6 on NetBSD 2.0 Release. Our measurement results showed νLIN6 can provide host mobility and network mobility with low overhead.

  6. Antiviral compounds and one new iridoid glycoside from Cornus officinalis

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    From Cornus officinalis Sieb. et Zucc., bioassay-guided fractionation led to the isolation of four active tannin compounds with high effectiveness of inhibiting Hepatitis C virus NS3 serine protease in vitro. The compounds are: 1, 2, 3, 6-tetragalloyl-β-D-glucopyranose ( 1 ), 1, 2, 3, 4, 6- pentagalloyl-β-D-glucopyranose (2), Tellimagrandin Ⅰ (3) and Tellimagrandin Ⅱ (4). The four compounds could inhibit HCV NS3 protease in vitro with IC50 values of 6.98, 5.11, 7.0 and 4.8 μmol/L respectively. In addition, a new iridoid glycoside (5) was also isolated from Cornus officinalis Sieb. et Zucc., which was assigned to be 7-O-butyl morroniside by spectroscopic analysis.

  7. Rosmarinus officinalis Extract Suppresses Propionibacterium acnes–Induced Inflammatory Responses

    OpenAIRE

    Tsai, Tsung-Hsien; Chuang, Lu-Te; Lien, Tsung-Jung; Liing, Yau-Rong; Chen, Wei-Yu; Tsai, Po-Jung

    2013-01-01

    Propionibacterium acnes is a key pathogen involved in the progression of acne inflammation. The development of a new agent possessing antimicrobial and anti-inflammatory activity against P. acnes is therefore of interest. In this study, we investigated the inhibitory effect of rosemary (Rosmarinus officinalis) extract on P. acnes–induced inflammation in vitro and in vivo. The results showed that ethanolic rosemary extract (ERE) significantly suppressed the secretion and mRNA expression of pro...

  8. [Antidepressant active constituents in the roots of Morinda officinalis How].

    Science.gov (United States)

    Cui, C; Yang, M; Yao, Z; Cao, B; Luo, Z; Xu, Y; Chen, Y

    1995-01-01

    Five compounds having antidepressant activities have been isolated from the roots of Morinda officinalis, a Chinese traditional Yang-tonic drug. These compounds were identified as succinic acid (1), nystose (2), 1F-fructofuranosylnystose (3), inulin-type hexasaccharide (4) and heptasaccharide (5) by chemical and spectroscopic methods. All of the compounds are isolated from the species of genus Morinda for the first time. PMID:7626209

  9. Effect of Biofertilizers on Agronomic Criteria of Hyssop (Hyssopus officinalis)

    OpenAIRE

    Tabrizi, Leila; Alireza KOOCHEKI; Ghorbani, Reza

    2008-01-01

    An experiment was conducted under field conditions to evaluate the effects of pure or combinations of biofertilizers on agronomic and quality criteria of Hyssop (Hyssopus officinalis), a medicinal and aromatic plant from Labiateae family at the Research Station of the Faculty of Agriculture, Ferdowsi University of Mashhad, during 2006 and 2007. A complete randomized block design with three replications was used. Treatments containing Azospirillum/Azotobacter(Nitroxin), Azospirillum/Bacillus s...

  10. Meiosis of anther culture regenerants in asparagus (Asparagus officinalis L.)

    OpenAIRE

    Leonardo Galli; Judith Viégas; Eliane Augustin; Marcia Ines Eckert; João Baptista da Silva

    1998-01-01

    Pollen mother cells obtained from regenerated plants of asparagus (Asparagus officinalis L.), in a population composed exclusively of male plants, through the process of anther culture from the hybrid G27 X 22-8, were analyzed during meiosis. It was observed that, during theprocess of anther culture by organogenesis, the pollen mother cells of the regenerants had great genomic instability, as evidenced by disturbances in all the meiotic phases of the first and second division. Furthermore, st...

  11. Determination Of Antioxidant Activities In Freshliver (Salvia Officinalis) Plant

    OpenAIRE

    Arıduru, Rana; Arabacı, Gülnur

    2013-01-01

    In this study, we determined the antioxidant activities of four different solvent fractions (ethanol, methanol, acetone and ethyl acetate) obtained from Freshliver plant leaves (Salvia officinalis) by employing two different assays such as 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) and Folin-Ciocaltaeu method. The results showed that ethanolextract of freshliver plant exhibited the highest total phenolic contents (43.55 mg GAE/g extract), followed by methanol-extract of freshliver plant (23...

  12. ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY ON EMBLICA OFFICINALIS SEED EXTRACT

    OpenAIRE

    Gupta Priya; Nain Parminder; Sidana Jaspreet

    2012-01-01

    The present study was carried out to evaluate in vitro antibacterial and free radical scavenging activity of methanolic extract of Emblica officinalis seed. The antimicrobial activity was assessed against gram positive and gram negative bacteria namely E.coli, P.aeruginosa, K.pneumoniae, S. aureus, Enterococcus by using agar well diffusion method. The antioxidant activity of seed extract was evaluated by using the free radical scavenging activity assay i.e DPPH method, hydrogen peroxide and r...

  13. A new approach to control the modified LinVerter for high frequency applications

    DEFF Research Database (Denmark)

    Farhang, Peyman; Mátéfi-Tempfli, Stefan

    2016-01-01

    In this paper not only a modified multi-device LinVerter is proposed but also a novel approach to control this topology is introduced. This topology is able to improve the conventional LinVerter performance using parallel power devices and designing a sequential switching scheme. In addition, from...... control perspective, a novel control approach based on bidirectional interface between LTspice and MATLAB is created. In this case, the circuit is modeled in LTspice environment and a Chaos Optimization Algorithm (COA) is coded in MATLAB in order to find out the optimal solution in control process....... In fact, this new approach combines the advantages of LTspice for simulation of different circuit configurations using actual component obtained from manufactures' models with advanced intelligent techniques capabilities from COA in MATLAB. First, the performance of proposed multi-device LinVerter along...

  14. Run-time system based on LinSERCANS and Soft-PLC

    Institute of Scientific and Technical Information of China (English)

    Cunfeng KANG; Chong WANG; Chunmin MA; Xudong HUANG; Renyuan FEI

    2009-01-01

    Soft-PLC with open architecture is the direction of development in industrial automation. This paper discusses the method of communication between the interface functions of LinSERCANS under RTLinux and the external library of Soft-PLC under Windows. Based on the API HOOK theory, the communication between the interface functions of LinSERCANS and the external libraries of Soft-PLC is set up and it solves the calling functions of dynamic link libraries in different operation systems. It is able to combine LinSERCANS with the Soft-PLC, and a run-time system is developed based on the interface technology of the serial real-time communication system (SERCOS) and technology of soft-PLC. This run-time system has been used in all electronic injection molding machines and works very well.

  15. Lin Zhichun's Contributions to the Study of Ancient World History in China

    Institute of Scientific and Technical Information of China (English)

    Yan; Shaoxiang

    2015-01-01

    Lin Zhichun (林志纯, 1910-2007, henceforthreferred to as Lin, but popularly known by hispen name of Ri Zhi), was a famous historian ofancient world history in China. Born in Fujian,he was enrolled in The Great China Universityin 1939 as a junior college student, and becamean undergraduate in 1941. Lin probably knewwhat he would do after graduation and learntEnglish, Russian and Latin in the university.1But the turmoil in China in the next 10 years(Japanese invasion of China since 1937 and thenthe Liberation War) made his life rather hard andhe had to move between Shanghai, Beijing and Shenyang successively in order tosupport himself. However, he tried to carry out his researches on Chinese history andpublished some articles since 1934.

  16. In vitro effects of Salvia officinalis L. essential oil on Candida albicans

    Directory of Open Access Journals (Sweden)

    Tularat Sookto

    2013-05-01

    Conclusions: S. officinalis L. essential oil exhibited anticandidal activities against C. albicans and had inhibitory effects on the adhesion of the cells to PMMA resin surface. With further testing and development, S. officinalis essential oil may be used as an antifungal denture cleanser to prevent candidal adhesion and thus reduce the risk of candida-associated denture stomatitis.

  17. Kinetic and sequence-structure-function analysis of known LinA variants with different hexachlorocyclohexane isomers.

    Directory of Open Access Journals (Sweden)

    Pooja Sharma

    Full Text Available BACKGROUND: Here we report specific activities of all seven naturally occurring LinA variants towards three different isomers, α, γ and δ, of a priority persistent pollutant, hexachlorocyclohexane (HCH. Sequence-structure-function differences contributing to the differences in their stereospecificity for α-, γ-, and δ-HCH and enantiospecificity for (+- and (--α -HCH are also discussed. METHODOLOGY/PRINCIPAL FINDINGS: Enzyme kinetic studies were performed with purified LinA variants. Models of LinA2(B90A A110T, A111C, A110T/A111C and LinA1(B90A were constructed using the FoldX computer algorithm. Turnover rates (min(-1 showed that the LinAs exhibited differential substrate affinity amongst the four HCH isomers tested. α-HCH was found to be the most preferred substrate by all LinA's, followed by the γ and then δ isomer. CONCLUSIONS/SIGNIFICANCE: The kinetic observations suggest that LinA-γ1-7 is the best variant for developing an enzyme-based bioremediation technology for HCH. The majority of the sequence variation in the various linA genes that have been isolated is not neutral, but alters the enantio- and stereoselectivity of the encoded proteins.

  18. Metabolism of monoterpanes: metabolic fate of (+)-camphor in sage (Salvia officinalis). [Salvia officinalis

    Energy Technology Data Exchange (ETDEWEB)

    Croteau, R.; El-Bialy, H.; Dehal, S.S.

    1987-07-01

    The bicyclic monoterpene ketone (+)-camphor undergoes lactonization to 1,2-campholide in mature sage (Salvia officinalis L.) leaves followed by conversion to the ..beta..-D-glucoside-6-O-glucose ester of the corresponding hydroxy acid (1-carboxymethyl-3-hydroxy-2,2,3-trimethyl cyclopentane). Analysis of the disposition of (+)-(G-/sup 3/H)camphor applied to midstem leaves of intact flowering plants allowed the kinetics of synthesis of the bis-glucose derivative and its transport from leaf to root to be determined, and gave strong indication that the transport derivative was subsequently metabolized in the root. Root extracts were shown to possess ..beta..-glucosidase and acyl glucose esterase activities, and studies with (+)-1,2(U-/sup 14/C)campholide as substrate, using excised root segments, revealed that the terpenoid was converted to lipid materials. Localization studies confirmed the radiolabeled lipids to reside in the membranous fractions of root extracts, and analysis of this material indicated the presence of labeled phytosterols and labeled fatty acids (C/sub 14/ to C/sub 20/) of acyl lipids. Although it was not possible to detail the metabolic steps between 1,2-campholide and the acyl lipids and phytosterols derived therefrom because of the lack of readily detectable intermediates, it seemed likely that the monoterpene lactone was degraded to acetyl CoA which was reincorporated into root membrane components via standard acyl lipid and isoprenoid biosynthetic pathways. Monoterpene catabolism thus appears to represent a salvage mechanism for recycling mobile carbon from senescing oil glands on the leaves to the roots.

  19. Acaricidal and Ovicidal Effects of Sage (Salvia officinalis L.) and Rosemary (Rosmarinus officinalis L.) (Lamiaceae) Extracts on Tetranychus urticae Koch (Acari: Tetranychidae)

    OpenAIRE

    Sibel YORULMAZ SALMAN; Sarıtaş, Semiha; KARA, Nimet; Ay, Recep

    2014-01-01

    Tetranychus urticae Koch (Acari: Tetranychidae) is a harmful pest for crops such as vegetables, fruits, and ornamental and industrial plants. The usage of plant extracts for pest control is seen as an alternative to synthetic pesticides. The effect of methanolic extracts obtained from sage (S. officinalis) and rosemary (R. officinalis) plants from the Lamiaceae family on T. urticae was researched in an effort to create an alternative to synthetic pesticides. The spray tower-leaf disk method w...

  20. Combustion of Pure, Hydrolyzed and Methyl Ester Formed of Jatropha Curcas Lin oil

    Directory of Open Access Journals (Sweden)

    Muhaji Muhaji

    2015-10-01

    Full Text Available The density and viscosity of vegetable oil are higher than that of diesel oil. Thus its direct combustion in the diesel engine results many problems. This research was conducted to investigate the flame characteristics of combustion of jatropha curcas lin in pure, hydrolyzed and methyl ester form. The results indicated that the combustion of pure jatropha curcas lin occurs in three stages, hydrolyzed in two stages    and methyl ester in one stage. For pure jatropha curcas lin, in the first stage, unsaturated fatty acid burned for  0.265 s.  It is followed by saturated fatty acid, burned for 0.389 s in the second stage. And, in the last stage is the burned of glycerol for 0.560 s. Meanwhile for hydrolyzed one, in the first stage, unsaturated fatty acid burned for 0.736 s, followed by saturated fatty acid, burned  for 0.326 s in the second stage. And the last, for methyl ester is the burned for 0.712 s. The highest burning rate was for methyl ester which was 0.003931cc/s. The energy releasing rate of methyl ester, which was for 13,628.67 kcal/(kg.s resembled that of diesel oil the most, while the lowest rate was for pure jatropha curcas lin which was 8,200.94 kcal/(kg.s. In addition, massive explosion occurred in the fuel containing unsaturated fatty acid and glycerol

  1. linäituse stendid = Outdoor exhibition stands / Gert Sarv, Galina Burnakova

    Index Scriptorium Estoniae

    Sarv, Gert, 1971-

    2013-01-01

    Eesti Sisearhitektide Liidu 2012. aasta parima eseme preemia pälvinud välinäituse stendidest Vanasadama Admiraliteedi basseini kaldapealsel. Autorid: Gert Sarv, Galina Burnakova, Loreida Hein. Sisearhitekti ja ESLi aastapreemiate žürii liikme Tea Tammelaane arvamus

  2. The Value of SCMC in SLA: Comments on Lin, Huang & Liou (2013)

    Science.gov (United States)

    Taylor, Alan M.

    2014-01-01

    Meta-analytic methods are often used to determine the effectiveness of certain treatments across studies. However, we are often unaware of how a meta-analysis can provide value to researchers and practitioners. This paper offers a brief commentary on a meta-analysis conducted by Lin, Huang and Liou (2013) in LLT, providing further statistical…

  3. Single-tube linear DNA amplification (LinDA) for robust ChIP-seq

    NARCIS (Netherlands)

    Shankaranarayanan, P.; Mendoza-Parra, M.A.; Walia, M.; Wang, L.; Li, N.; Trindade, L.M.; Gronemeyer, H.

    2011-01-01

    Genome-wide profiling of transcription factors based on massive parallel sequencing of immunoprecipitated chromatin (ChIP-seq) requires nanogram amounts of DNA. Here we describe a high-fidelity, single-tube linear DNA amplification method (LinDA) for ChIP-seq and reChIP-seq with picogram DNA amounts

  4. An Adaptation of the Kernighan-Lin Heuristic to the Simple Graph Partitioning Problem

    DEFF Research Database (Denmark)

    Sørensen, Michael Malmros

    1999-01-01

    problem of the Kernighan-Lin exchange heuristic, which was originally developed for the closely related 2-partition problem. The evaluation is carried out on problem instances on graphs with up to 50 nodes for which the optimal partition values are known or upper bounds are available. The computational...

  5. Function of RNA-binding Protein Lin28 in Germ Cell Development and Sex Maturation%RNA结合蛋白Lin28在生殖细胞发育和性成熟过程中的作用

    Institute of Scientific and Technical Information of China (English)

    马薇

    2013-01-01

    As a critical heterochronic gene family protein, RNA-binding protein Lin28 and its homologue Lin-28B play an important role in primordial germ cells (PGCs) development, germ cell tumors (GCTs) malignancy and sex maturation. Lin28 binds to the terminal loops of let-7 miRNA precursors, leading to inhibition of let-7 miRNA processing and the induction of uridylation and precursor degradation. Lin28 also selectively binds to the target mRNAs and stimulates their translation. Lin28 is essencial for proper PGCs development and has temporal and spatial expression pattern during PGC development in mouse embryo. Lin28 can both positively and negatively regulate the pool of germ cell numbers. Aberrant expression of LIN28 may be an early lesion in germ cell tumorigenesis. Lin28 regulates the differentiation status of seminorma, embryonal carcinoma likely upsteam of Oct3/4 and Nanog, and is likely to play a related role in normal human germ cell development. In addition, Lin28B gene is associated with earlier breast development in girls, earlier voice breaking and more advanced pubic hair development in boys and faster tempo of height growth in both of them. This review summarizes Lin28-medicated regulation and possible mechanisms on germ cell development and sex maturation.%作为一个关键的异时性基因(heterochronic gene)家族成员蛋白,Lin28及其同源分子Lin28B在原始生殖细胞(PGCs)发生、生殖细胞肿瘤(GCTs)恶化和性发育成熟过程中发挥着重要的调控作用.Lin28不但可与let-7 miRNA家族的前体终末环相结合,从而阻断let-7miRNA的成熟过程,而且可诱导let-7前体尿苷化和降解.同时,Lin28也可选择性地结合mRNA靶基因,从而直接刺激该基因的翻译过程.Lin28对于PGCs的发育分化至关重要,其表达在小鼠PGCs发育过程中具有时空限制性,还可通过正、负双向途径调节PGC池内生殖细胞数.LIN28的异常表达可能是恶性GCTs发生的早期信号.Lin28还可能是Oct3

  6. Rosmarinus officinalis L. as cause of contact dermatitis.

    Science.gov (United States)

    Miroddi, M; Calapai, G; Isola, S; Minciullo, P L; Gangemi, S

    2014-01-01

    Because of the widespread use of botanicals, it has become crucial for health professionals to improve their knowledge about safety problems. Several herbal medicines contain chemicals with allergenic properties responsible for contact dermatitis. Among these, one is Rosmarinus officinalis L. (rosemary), a plant used since ancient times in folk medicine; at the present time it is used worldwide as a spice and flavouring agent, as a preservative and for medicinal and cosmetic purposes. The present article aims to revise and summarise scientific literature reporting cases of contact dermatitis caused by the use of R. officinalis as a raw material or as herbal preparations. Published case reports were researched on the following databases and search engines: PUBMED, MEDLINE, EMBASE, Google Scholar, Scopus. The used keywords were: R. officinalis and rosemary each alone or combined with the words allergy, contact dermatitis, allergic contact dermatitis, sensitisation and occupational dermatitis. The published case reports show that both rosemary extracts and raw material can be responsible for allergic contact dermatitis. Two cases related to contact dermatitis caused by cross-reactivity between rosemary and thyme were also commented. The diterpene carnosol, a chemical constituent of this plant, has been imputed as a common cause for this reaction. The incidence of contact dermatitis caused by rosemary is not common, but it could be more frequent with respect to the supposed occurrence. It seems plausible that cases of contact dermatitis caused by rosemary are more frequent with respect to the supposed occurrence, because they could be misdiagnosed. For this reason, this possibility should be carefully considered in dermatitis differential diagnosis. PMID:23827646

  7. Phenolic compounds from the roots of Valeriana officinalis var. latifolia

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Peng-Cheng; Ran, Xin-Hui; Luo, Huai-Rong; Liu, Yu-Qing; Zhou Jun [State Key Laboratory of Phytochemistry and Plant Resources in West China. Kunming Institute of Botany, Chinese Academy of Sciences (China); Ma, Qing-Yun; Zhao, You-Xing, E-mail: zhoujun3264@yahoo.com.cn, E-mail: zhaoyouxing@itbb.org.cn [Key Laboratory of Biology and Genetic Resources of Tropical Crops. Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology. Chinese Academy of Tropical Agriculture Sciences (China)

    2013-09-15

    A new benzofuran neolignan, dihydrodehydrodiconiferyl alcohol 9-isovalerate, along with ten known phenolic compounds, olivil, pinoresinol, 8-hydroxypinoresinol, pinorespiol, 8-hydroxy- 7-epipinoresinol, trans-p-hydroxyphenyl- propenoic acid, cis-p-hydroxyphenyl-propenoic acid, ferulic acid, isoferulic acid and isovanillin were isolated from the roots of Valeriana officinalis var. latifolia. Their structures and configurations were elucidated on the basis of spectroscopic methods. The inhibitory activity for acetylcholinesterase (AChE) and enhancing activity on nerve growth factor (NGF)-mediated neurite outgrowth in PC12 cells of dihydrodehydrodiconiferyl alcohol 9-isovaterate and olivil were evaluated. (author)

  8. ANTIOXIDANT, IMMUNOMODULATORY AND ANTICANCER ACTIVITIES OF EMBLICA OFFICINALIS: AN OVERVIEW

    Directory of Open Access Journals (Sweden)

    Madhuri S.

    2011-08-01

    Full Text Available Emblica officinalis (Amla is traditionally used for several diseases, and it is believed to increase the defense against diseases. It is particularly used for the treatment of cancer, diabetes, liver disorders, heart disease, ulcer, snake venom, haemorrhage, diarrhea, dysentery, anaemia and ophthalmic disorders. The antioxidant, immunomodulatory, anticancer, cytoprotective, analgesic, antimicrobial, antipyretic, antitussive and gastroprotective are the important properties of amla. Vitamin C, tannins and flavaniods present in amla have very powerful antioxidant activities. Due to rich vitamin C, amla is successfully used in the treatment of human scurvy.

  9. Determination Of Antioxidant Activities In Freshliver (Salvia Officinalis) Plant

    OpenAIRE

    Rana Arıduru; Gülnur Arabacı

    2013-01-01

    In this study, we determined the antioxidant activities of four different solvent fractions obtained from Freshliver plant leaves (Salvia officinalis) by employing two different assays such as 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) and Folin-Ciocaltaeu method. The results showed that ethanol-extract of freshliver plant exhibited the highest total phenolic contents mg GAE/g extract; 43,55, methanol-extract of 23.62, ethyl acetate extract 18.29, and acetone extract 11.58. All the extracti...

  10. Metformin may antagonize Lin28 and/or Lin28B activity, thereby boosting let-7 levels and antagonizing cancer progression.

    Science.gov (United States)

    McCarty, Mark F

    2012-02-01

    Cancer cells with stem cell characteristics are harbored by most tumors, and are characterized by epithelial-mesenchymal transition (EMT) - which promotes invasive growth and metastasis - chemoresistance, and the capacity to reconstitute new tumors. Hence, the control or destruction of cancer stem cells should be a major goal of cancer management. The let-7 family of microRNAs has cancer suppressor activity, and recent evidence suggests that markedly reduced levels of let-7 are not only a typical feature of cancer stem cells, but may be largely responsible for cancer stemness. It is therefore particularly intriguing that metformin, a diabetes drug thought to have potential in the prevention and treatment of cancer, has recently been found to oppose cancer cell stemness, to markedly potentiate chemotherapeutic control of cancer in mouse xenograft models, and to notably boost let-7a levels in cancer stem cells. It is proposed that this latter effect of metformin may reflect AMPK-mediated inhibition of the expression or activity of Lin28/Lin28A, proteins which act post-transcriptionally to decrease the levels of all let-7 family members. The transcription of Lin28B is promoted by NF-kappaB and by Myc; hence, practical measures which antagonize NF-kappaB or Myc activity may complement the utility of metformin for boosting let-7 expression and controlling cancer stemness; salsalate, antioxidants, tyrosine kinase and cox-2 inhibitors, ribavirin, vitamin D, gamma-secretase inhibitors (when available), and parenteral curcumin may have some utility in this regard. Although the impact of histone deacetylase inhibitors on let-7 expression has not been assessed, there is reason to suspect that these drugs might complement let-7's impact on chemoresistance, EMT, and stemness. Multifocal strategies centering on metformin may have considerable potential for reversing cancer stemness and rendering advanced cancers more susceptible to long term control. PMID:22129484

  11. [Determination of polysaccharide from Chinese medicine Morinda officinalis how and its trace elements analysis].

    Science.gov (United States)

    Wu, Yong-Jun; Liu, Jie; Wu, Yu-Ming; Liu, Li-E; Zhang, Hong-Quan

    2005-12-01

    Polysaccharide was extracted from morinda officinalis how by back-flowing with 80% ethanol for 1 h, and the method of improved phenol-sulfuric acid spectroscopy was adopted to determine the content of morinda officinalis how polysaccharide. As for its trace elements, the atomic absorption spectroscopy was used to detect the content of Zn, Fe and Cu, which were compared with its fake; and cold atomic fluorometry was applied to determine the trace mercury. The results were satisfactory, which can give reference about the effective components of morinda officinalis how, and will help to exploit it. PMID:16544510

  12. Meiosis of anther culture regenerants in asparagus (Asparagus officinalis L.

    Directory of Open Access Journals (Sweden)

    Leonardo Galli

    1998-03-01

    Full Text Available Pollen mother cells obtained from regenerated plants of asparagus (Asparagus officinalis L., in a population composed exclusively of male plants, through the process of anther culture from the hybrid G27 X 22-8, were analyzed during meiosis. It was observed that, during theprocess of anther culture by organogenesis, the pollen mother cells of the regenerants had great genomic instability, as evidenced by disturbances in all the meiotic phases of the first and second division. Furthermore, structural chromosomal abnormalities, in addition to aneuploidy and polyploidy, were observed.Foi analisada a meiose em células mãe de pólen de plantas de aspargo (Asparagus officinalis L. de uma população composta exclusivamente de plantas masculinas, obtidas através do processo de cultura de anteras do híbrido G27 X 22-8. Foi observado que, durante o processo de cultura de anteras, via calogênese, as células mãe de pólen dos regenerantes apresentaram grande instabilidade genômica, evidenciada por irregularidades nas fases de diacinese, assim como de metáfase, anáfase, telófase da primeira e segunda divisão meiótica. Além disto, o processo originou anormalidades cromossômicas estruturais em adição às aneuploidias e poliploidias.

  13. Ecophysiological and antioxidant traits of Salvia officinalis under ozone stress.

    Science.gov (United States)

    Pellegrini, Elisa; Francini, Alessandra; Lorenzini, Giacomo; Nali, Cristina

    2015-09-01

    Ecophysiological and antioxidant traits were evaluated in sage (Salvia officinalis) plants exposed to 120 ppb of ozone for 90 consecutive days (5 h day(-1)). At the end of fumigation, plants showed slight leaf yellowing that could be considered the first visual symptom of leaf senescence. Ozone-stressed leaves showed (1) reduced photosynthetic activity (-70 % at the end of exposure), (2) chlorophyll loss (-59 and -56 % of chlorophyll a and b concentrations, starting from 30 days from the beginning of exposure), and (3) cellular water deficit (-12 % of the relative water content at the end of the fumigation). These phenomena are indicative of oxidative stress in the chloroplasts (as confirmed by the strong degradation of β-carotene) despite the photoprotection conferred by xanthophyll cycle [as demonstrated by the significant rise of de-epoxidation index, reaching the maximum value at the end of the treatment (+69 %)], antioxidant compounds [as confirmed by the increase of phenols (in particular caffeic acid and rosmarinic acid)], and water-soluble carbohydrates (especially monosaccharides). By means of combined ecophysiological and biochemical approaches, this study demonstrates that S. officinalis is able to activate an adaptive survival mechanism allowing the plant to complete its life cycle even under oxidative stressful conditions.

  14. Promotion of hair growth by Rosmarinus officinalis leaf extract.

    Science.gov (United States)

    Murata, Kazuya; Noguchi, Kazuma; Kondo, Masato; Onishi, Mariko; Watanabe, Naoko; Okamura, Katsumasa; Matsuda, Hideaki

    2013-02-01

    Topical administration of Rosmarinus officinalis leaf extract (RO-ext, 2 mg/day/mouse) improved hair regrowth in C57BL/6NCrSlc mice that experienced hair regrowth interruption induced by testosterone treatment. In addition, RO-ext promoted hair growth in C3H/He mice that had their dorsal areas shaved. To investigate the antiandrogenic activity mechanism of RO-ext, we focused on inhibition of testosterone 5α-reductase, which is well recognized as one of the most effective strategies for the treatment of androgenic alopecia. RO-ext showed inhibitory activity of 82.4% and 94.6% at 200 and 500 µg/mL, respectively. As an active constituent of 5α-reductase inhibition, 12-methoxycarnosic acid was identified with activity-guided fractionation. In addition, the extract of R. officinalis and 12-methoxycarnosic acid inhibited androgen-dependent proliferation of LNCaP cells as 64.5% and 66.7% at 5 µg/mL and 5 μM, respectively. These results suggest that they inhibit the binding of dihydrotestosterone to androgen receptors. Consequently, RO-ext is a promising crude drug for hair growth. PMID:22517595

  15. Rosmarinic Acid and Melissa officinalis Extracts Differently Affect Glioblastoma Cells

    Directory of Open Access Journals (Sweden)

    Kristina Ramanauskiene

    2016-01-01

    Full Text Available Lemon balm (Melissa officinalis L. has many biological effects but especially important is its neuroprotective activity. The aim of the study is to produce different extracts of Melissa officinalis and analyse their chemical composition and biological properties on rat glioblastoma C6 cells. Results revealed that rosmarinic acid (RA is the predominant compound of lemon balm extracts. RA has cytotoxic effect on glioblastoma cells (LC50 290.5 μM after the incubation of 24 h and LC50 171.3 μM after 48 h. RA at concentration 80–130 μM suppresses the cell proliferation and has an antioxidant effect. 200 μM and higher concentrations of RA have a prooxidant effect and initiate cell death through necrosis. The aqueous extract of lemon balm is also enriched in phenolic compounds: protocatechuic, caftaric, caffeic, ferulic, and cichoric acids and flavonoid luteolin-7-glucoside. This extract at concentrations 50 μM–200 μM RA has cytotoxic activity and initiates cell death through apoptosis. Extracts prepared with 70% ethanol contain the biggest amount of active compounds. These extracts have the highest cytotoxic activity on glioblastoma cells. They initiate generation of intracellular ROS and cell death through apoptosis and necrosis. Our data suggest that differently prepared lemon balm extracts differently affect glioblastoma cells and can be used as neuroprotective agents in several therapeutic strategies.

  16. A Comparative Study on Different Fates between Jane Eyre and Lin Daiyu

    Institute of Scientific and Technical Information of China (English)

    林冬梅; 赵慧

    2014-01-01

    Jane Eyre and A Dream of Red Mansions can be regarded as the most famous two classics in the history of literature. In A Dream of Red Mansions, Cao Xueqin exhausted almost all his heart and soul to portray the character─Lin Daiyu, whose tragic and impressive love story has moved thousands of readers into tears. While, Charlotte Bronte ’s Jane Eyre raised heated discussion in 19th century because of the vivid and energetic female she depicted in her book. By comparison, it is easy to find that there are some similarities between the two characters. However, their fates are quite different. This paper tries to make a comparison be-tween Jane Eyre and Lin Daiyu, review their life experience, analyze possible reasons that may account for the different fates, and draw a conclusion. Hope that this paper can do some help to those who are interested in literature home and abroad.

  17. Structural and electronic properties of stable Lin (n=2-10) clusters: A density functional study

    Science.gov (United States)

    Chetri, Pawan; Deka, Ramesh Ch.; Choudhury, Amarjyoti

    2013-12-01

    Structure and relative stability of Lin clusters for n=1-10 were investigated using density functional methods based DMol3 program. The structures of Li clusters were determined in terms of Li-Li bond length and the results are in very good agreement with experimental values. Stability of the clusters was determined from their relative energy values binding energies and second difference energy. We also determined fragmentation energy of each size of cluster. We arrived at some interesting result like the transition of Lin from 2-dimension to 3-dimension for a particular value of n and also the variation of HOMO-LUMO gap with cluster size. Many structures are characterized for the first time in this work.

  18. An Algorithmic Complexity Interpretation of Lin's Third Law of Information Theory

    Directory of Open Access Journals (Sweden)

    Joel Ratsaby

    2008-03-01

    Full Text Available Instead of static entropy we assert that the Kolmogorov complexity of a static structure such as a solid is the proper measure of disorder (or chaoticity. A static structure in a surrounding perfectly-random universe acts as an interfering entity which introduces local disruption in randomness. This is modeled by a selection rule R which selects a subsequence of the random input sequence that hits the structure. Through the inequality that relates stochasticity and chaoticity of random binary sequences we maintain that Lin’s notion of stability corresponds to the stability of the frequency of 1s in the selected subsequence. This explains why more complex static structures are less stable. Lin’s third law is represented as the inevitable change that static structure undergo towards conforming to the universe’s perfect randomness.

  19. Manipulation quantique de la lumière par un amplificateur non linéaire

    Science.gov (United States)

    Symul, T.; Bencheikh, K.; Levenson, J. A.

    2002-06-01

    Nous proposons un dispositif original, appelé Amplificateur Non Linéaire (ANL), permettant la génération et la manipulation d'états quantiques de la lumière. Ce dispositif permet une compression du bruit quantique de la lumière en dessous de la limite quantique standard plus efficace que celle obtenue par interactions non linéaires du second ordre ou du troisième ordre. Il permet également d'inverser les fluctuations quantiques en intensité de la lumière, et de produire des photons jumeaux ayant des corrélations quantiques plus élevées et plus robustes que ceux produits par un amplificateur paramétrique seul.

  20. Fragmented Encounters, Social Slippages: Lin Huiyin's "In Ninety-Nine Degree Heat"

    Directory of Open Access Journals (Sweden)

    Carles Prado-Fonts

    2010-01-01

    Full Text Available The article reads Lin Huiyin’s short story “In Ninety-Nine Degree Heat” (1934 in relation to the context of 1930s China, as an innovative literary work which combines elements from both the Chinese and the Western traditions, and as a text which informs readers not only of the problematic of class and gender issues in 1930s Chinese society but also of the context of the liuxuesheng who returns to China –like Lin Huiyin herself. Focusing on questions like otherness, representation, and encounters, the essay analyzes how the episodic narrative structure of Lin’s short story echoes social and representational discourses in post-May Fourth China, at the same time that it explores issues such as social inequality, otherness and alienation, which were crucial to the liuxuesheng, and which reflect Lin’s own experience as a returned and alienated liuxuesheng at the time.

  1. [Exploring the opium prohibition campaign led by LIN Ze-xu, a medical perspective].

    Science.gov (United States)

    Zhang, Sun-Biao; Wang, Ping

    2013-03-01

    Opium destruction in Humen, a feat of the national hero, LIN Ze-xu turned a new page of modern history of China. In the past researches, the campaign always basically studied under a political historical background. In fact, it was also related closely to medicine. During the reign of the Dao Guang Emperor of the Qing Dynasty, there was always mentioning of the risk of doping opium and its addiction process and mechanism in his serial reports and letters to the imperial government. During this inhibition process, he took measures, such as providing the medicines to conquer the addiction of opium, asking the effective prescriptions for prohibiting opium from physicians and strove to popularize them. As viewed from a medical and historical perspective, the opium prohibition campaign led by LIN Ze-xu still reveals some enlightening significance even for the current society. PMID:24135477

  2. GENII-LIN: a Multipurpose Health Physics Code Built on GENII-1.485

    Directory of Open Access Journals (Sweden)

    Marco Sumini

    2006-10-01

    Full Text Available The aim of the GENII-LIN project was to develop an open source multipurpose health physics code running on Linux platform, for calculating radiation dose and risk from radionuclides released to the environment. The general features of the GENII-LIN system include [1] capabilities for calculating radiation dose both for acute and chronic releases, with options for annual dose, committed dose and accumulated dose [2] capabilities for evaluating exposure pathways including direct exposure via water (swimming, boating, fishing, soil (buried and surface sources and air (semi-infinite cloud and finite cloud model, inhalation pathways and ingestion pathways. The release scenarios considered are: - acute release to air, from ground level or elevated sources, or to water; - chronic release to air, from ground level or elevated sources, or to water; - initial contamination of soil or surfaces. Keywords: radiation protection, Linux, health physics, risk analysis.

  3. Šešėlinė ekonomika Lietuvoje ir jos vertinimas

    OpenAIRE

    Bertaitytė, Simona

    2007-01-01

    Oficialiai neapskaityta ekonomika apima visus oficialiosios statistikos neapskaitytus, bet į BVP įskaičiuojamus sandorius. Neapskaitoma ekonomika 2005 m. sudarė 18.7 proc. šalies bendrojo vidaus produkto. Šešėlinė ekonomika iškreipia oficialiąją statistiką, stabdo ekonomikos augimą, todėl būtina ją tirti, fiksuoti, ieškoti būdų ją pažaboti. Pagrindinis šio darbo tikslas - įvertinus šešėlinės ekonomikos susidarymo priežastis, šaltinius, mastą Lietuvoje, numatyti Lietuvai tinkami...

  4. Response to Niklasson's comment on Lin, et al. (2012) : "the relation between postural movement and bilateral motor integration".

    Science.gov (United States)

    Lin, Chin-Kai; Kuo, Bor-Chen; Wu, Huey-Min

    2014-10-01

    In the study of Lin, Wu, Lin, Wu, Wu, Kuo, and Yeung (2012 ), the relationship between the validity of postural movement and bilateral motor integration in terms of sensory integration theory was examined. Postural movement is the ability to use the antigravity postures required for stabilization of the neck, trunk and upper extremities via muscle co-contractions in the neck and upper extremities, and balance. Niklasson's (2013 ) comment argued that postural movement should include primitive reflexes in terms of the general abilities approach. Niklasson (2013 ) focused on the efficacy of the treatment rather than the theoretical frameworks implied in the therapeutic activities. For that purpose Lin, et al. (2012 ) used sensory integration as the theoretical foundation, and the relationship between postural movement and bilateral motor integration was assessed via empirical data. The result of Lin, et al. (2012 ) was offered as a theoretical reference for therapeutic activities.

  5. Reduction of EMC Emissions in Mixed Signal Integrated Circuits with Embedded LIN Driver

    OpenAIRE

    Hartl, P; M. Kuban; P. Horsky

    2016-01-01

    This paper describes several methods for reduction of electromagnetic emissions (EME) of mixed signal integrated circuits (IC). The focus is on the impact that a LIN bus communication block has on a complex IC which contains analog blocks, noisy digital block, micro-core (µC) and several types of memories. It is used in an automotive environment, where EMC emission reduction is one of the key success factors. Several proposed methods for EME reduction are described and implemented on three te...

  6. Lin4Neuro: a customized Linux distribution ready for neuroimaging analysis

    OpenAIRE

    Yamashita Fumio; Okamoto Masako; Tsuzuki Daisuke; Ohnishi Takashi; Rorden Christopher; Dan Ippeita; Nemoto Kiyotaka; Asada Takashi

    2011-01-01

    Abstract Background A variety of neuroimaging software packages have been released from various laboratories worldwide, and many researchers use these packages in combination. Though most of these software packages are freely available, some people find them difficult to install and configure because they are mostly based on UNIX-like operating systems. We developed a live USB-bootable Linux package named "Lin4Neuro." This system includes popular neuroimaging analysis tools. The user interfac...

  7. Baker- Lin-Huang type Bivariate distributions based on order statistics

    OpenAIRE

    Bairamov, I.; Bayramoglu, K.

    2011-01-01

    Baker (2008) introduced a new class of bivariate distributions based on distributions of order statistics from two independent samples of size n. Lin-Huang (2010) discovered an important property of Baker's distribution and showed that the Pearson's correlation coefficient for this distribution converges to maximum attainable value, i.e. the correlation coefficient of the Frech\\'et upper bound, as n increases to infinity. Bairamov and Bayramoglu (2011) investigated a new class of bivariate di...

  8. Relevance of the Lin's and Host hydropedological models to predict grape yield and wine quality

    OpenAIRE

    Costantini, E.A.C.; Pellegrini, S.; P. Bucelli; P. Storchi; N. Vignozzi; R. Barbetti; S. Campagnolo

    2009-01-01

    The adoption of precision agriculture in viticulture could be greatly enhanced by the diffusion of straightforward and easy to be applied hydropedological models, able to predict the spatial variability of available soil water. The Lin's and Host hydropedological models were applied to standard soil series descriptions and hillslope position, to predict the distribution of hydrological functional units in two vineyard and their relevance for grape yield and wine quality. A three-years trial w...

  9. Cobordism Obstructions to Vector Fields and a Generalization of Lin's Theorem

    DEFF Research Database (Denmark)

    Svane, Anne Marie

    Atiyah and Dupont have studied the existence of linearly independent vector fields on manifolds by means of K-theory. They obtained the complete conditions for up to three independent vector fields. In the thesis, we try to copy their approach using certain spectra related to cobordism theory. We...... generalizes Lin's inverse system in compact cases, while it behaves completely differently in the universal case....

  10. Phytochemical profile of Rosmarinus officinalis and Salvia officinalis extracts and correlation to their antioxidant and anti-proliferative activity.

    Science.gov (United States)

    Kontogianni, Vassiliki G; Tomic, Goran; Nikolic, Ivana; Nerantzaki, Alexandra A; Sayyad, Nisar; Stosic-Grujicic, Stanislava; Stojanovic, Ivana; Gerothanassis, Ioannis P; Tzakos, Andreas G

    2013-01-01

    The goal of this study was to monitor the anti-proliferative activity of Rosmarinus officinalis and Salvia officinalis extracts against cancer cells and to correlate this activity with their phytochemical profiles using liquid chromatography/diode array detection/electrospray ion trap tandem mass spectrometry (LC/DAD/ESI-MS(n)). For the quantitative estimation of triterpenic acids in the crude extracts an NMR based methodology was used and compared with the HPLC measurements, both applied for the first time, for the case of betulinic acid. Both extracts exerted cytotoxic activity through dose-dependent impairment of viability and mitochondrial activity of rat insulinoma m5F (RINm5F) cells. Decrease of RINm5F viability was mediated by nitric oxide (NO)-induced apoptosis. Importantly, these extracts potentiated NO and TNF-α release from macrophages therefore enhancing their cytocidal action. The rosemary extract developed more pronounced antioxidant, cytotoxic and immunomodifying activities, probably due to the presence of betulinic acid and a higher concentration of carnosic acid in its phytochemical profile.

  11. Synergistic antibacterial activity of Salvia officinalis and Cichorium intybus extracts and antibiotics.

    Science.gov (United States)

    Stefanović, Olgica D; Stanojević, Dragana D; Comić, Ljiljana R

    2012-01-01

    Synergistic activity of Salvia officinalis and Cichorium intybus extracts and commonly used antibiotics, amoxicillin and chloramphenicol, were evaluated. Interactions between plant extracts and antibiotics were tested by checkerboard method and interpreted as FIC index. Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and clinical isolates Staphylococcus aureus, Bacillus subtilis, Enterobacter cloacae, Klebsiella pneumoniae, Escherichia coli and Proteus mirabilis were used. Salvia officinalis showed better synergistic capacity than Cichorium intybus. Synergistic interactions were observed between amoxicillin and acetone or ethyl acetate extract of Salvia officinalis and between chloramphenicol and ethyl acetate extract of Salvia officinalis. In the presence of sub-inhibitory concentration (1/4 MIC to 1/32 MIC) of sage extracts, the MIC values of antibiotics were decreased by 2- to 10-fold. Synergism was observed against all test bacteria, except Escherichia coli. The combinations of acetone and ethyl acetate extract from Cichorium intybus and antibiotics resulted in additive and indifferent effects against tested bacteria.

  12. Antiviral activity of the volatile oils of Melissa officinalis L. against Herpes simplex virus type-2.

    Science.gov (United States)

    Allahverdiyev, A; Duran, N; Ozguven, M; Koltas, S

    2004-11-01

    Melissa officinalis L. (Lamiaceae) has been used in a variety of practical applications in medical science. Our objective in the current study was to determine the effects of the volatile oil components of M. officinalis on Herpes simplex virus type 2 (HSV-2) replication in HEp-2 cells. Four different concentrations (25, 50, 100, 150 and 200 microg/ml) of volatile oils were examined. Experiments were carried out using HEp-2 cells. M. officinalis volatile oil was found to be non-toxic to HEp-2 cells up to a concentration of 100 micro/ml. It was, however, found to be slightly toxic at a concentration over of 100 microg/ml. The antiviral activity of non-toxic concentrations against HSV-2 was tested. The replication of HSV-2 was inhibited, indicating that the M. officinalis L. extract contains an anti-HSV-2 substance. PMID:15636181

  13. Thesinine-4'-O-beta-D-glucoside the first glycosylated plant pyrrolizidine alkaloid from Borago officinalis.

    Science.gov (United States)

    Herrmann, Martina; Joppe, Holger; Schmaus, Gerhard

    2002-06-01

    The glycosylated pyrrolizidine alkaloid, thesinine-4'-O-beta-D-glucoside, has been isolated from the aqueous methanol extract of dried, defatted seeds of Borago officinalis (Boraginaceae). The structure was established by means of spectroscopic and chemical analysis. PMID:12031432

  14. [Relation between root structure and accumulation of anthraquinones of Morinda officinalis].

    Science.gov (United States)

    Yao, Hui; Wu, Hong; Feng, Cheng Hao; Zhao, Sheng; Liang, She Jian

    2004-04-01

    The histological structures of the roots of Morinda officinalis How of different ages were observed, the distribution and accumulation of anthraquinones in the root were studied with the help of paraffin section, fluorescent microscope and ultraviolet spectrophotometer. The results indicated that the mature structures of the roots of M. officinalis were similar to those of common perennial herbs, and that the anthraquinones in the root of M. officinalis distributed in parenchymatous cells and the content of anthraquinones in the root gradually increased in number with age. Based on above mentioned investigation, we came to the conclusion that the root of M. officinalis should be collected after four years of growing and the top grade root should have well developed phloem and thin xylem. PMID:15259981

  15. Dynamiques complexes et morphogenèse Introduction aux sciences non linéaires

    CERN Document Server

    Misbah, Chaouqi

    2011-01-01

    Les sciences non linéaires ont pour objet l’ensemble des phénomènes dont l’analyse résiste au principe de superposition. Elles concernent en grande partie les systèmes dits « complexes » dont l’interaction et l’interdépendance entre les parties empêchent de prédire précisément l’évolution du système. Pour expliquer ces phénomènes, deux approches complémentaires ont été proposées : la théorie des bifurcations et la théorie des catastrophes. Mais la pleine compréhension et la modélisation de la non-linéarité restent chacune un défi pour les scientifiques du XXIe siècle. C’est dans la perspective d’accompagner tous ceux qui voudront le relever que ce livre a été conçu. Son objectif est d’exposer au lecteur le langage et le formalisme nécessaires à l’étude de la non-linéarité. Partant d’exemples simples, pour ensuite atteindre un niveau d’abstraction visant l’universalité, l’auteur explore les divers scénarios possibles de bifurcations et les catastr...

  16. Differential regulation of the c-Myc/Lin28 axis discriminates subclasses of rearranged MLL leukemia

    Science.gov (United States)

    Chen, Lili; Sun, Yuqing; Wang, Jingya; Jiang, Hui; Muntean, Andrew G.

    2016-01-01

    MLL rearrangements occur in myeloid and lymphoid leukemias and are generally associated with a poor prognosis, however this varies depending on the fusion partner. We modeled acute myeloid leukemia (AML) in mice using various MLL fusion proteins (MLL-FPs) and observed significantly different survival outcomes. To better understand the differences between these leukemias, we examined the genome wide expression profiles of leukemic cells transformed with different MLL-FPs. RNA-sequencing and pathway analysis identified the c-Myc transcriptional program as one of the top distinguishing features. c-Myc protein levels were highly correlative with AML disease latency in mice. Functionally, overexpression of c-Myc resulted in a more aggressive proliferation rate in MLL-FP cell lines. While all MLL-FP transformed cells displayed sensitivity to BET inhibitors, high c-Myc expressing cells showed greater resistance to Brd4 inhibition. The Myc target Lin28B was also differentially expressed in MLL-FP cell lines in agreement with c-Myc expression. Examination of Lin28B miRNAs targets revealed that let-7g was significantly increased in leukemic cells associated with the longest disease latency and forced let-7g expression induced differentiation of leukemic blasts. Thus, differential regulation of the c-Myc/Lin28/let-7g program by different MLL-FPs is functionally related to disease latency and BET inhibitor resistance in MLL leukemias. PMID:27007052

  17. Green approach to corrosion inhibition of 304 stainless steel in hydrochloric acid solution by the extract of Salvia officinalis leaves

    International Nuclear Information System (INIS)

    Highlights: ► Salvia officinalis extract was examined as a corrosion inhibitor for steel. ► The inhibition efficiency increases with the increasing of extract concentration. ► Adsorption of S. officinalis extract on stainless steel surface is spontaneous. ► Activation energy decreases with addition of S. officinalis extract. - Abstract: The extract of Salvia officinalis (S. officinalis) leaves has been evaluated as a corrosion inhibitor for 304 stainless steel in 1 M HCl solution by means of weight loss measurements, potentiodynamic polarization, electrochemical impedance spectroscopy (EIS). Tafel polarization study revealed that extract of S. officinalis acts as a mixed type inhibitor. Adsorption of the inhibitor on the steel surface followed Langmuir adsorption isotherm. Quantum chemical calculations using the density functional theory (DFT) and semi-empirical method (AM1) were performed to illustrate the process of adsorption of some specific components of the extract.

  18. [Determination of antioxidation of the extract from Chinese medicine Morinda officinalis How by flow injection chemiluminescence and spectroscopy].

    Science.gov (United States)

    Wu, Yong-Jun; Shi, Jie; Qu, Ling-Bo; Li, Fei-Fei; Li, Xiao-Jing; Wu, Yi-Ming

    2006-09-01

    Flow injection chemiluminescence analysis was used to determine the antioxidation activity of extract from Morinda officinalis How. The determination was based on the inhibition effect of Morinda officinalis How extraction in the chemiluminescence reaction of luminol - H2O2-CuSO4 system, and vitamin C was used as positive control. It was showed that Morinda officinalis How original liquid has obvious anti-radical activity. Spectroscopy was applied to determine the inhibition ratio of Morinda officinalis How extraction for the superoxide anion and hydroxy radicals in the special system. It was shown that the extraction from Morinda officinalis How could obviously scavenge superoxide anion and hydroxy radicals. It is suggested that the Chinese Medicine Morinda Officinalis How is a potential antioxidation activity medicine. PMID:17112048

  19. NT-28NEUROPROTECTIVE EFFECTS OF BACOPA MONNIERI AND ROSMARINUS OFFICINALIS SUPERCRITICAL CO2 EXTRACTS

    OpenAIRE

    Ramachandran, Cheppail; Quirin, Karl-Werner; Escalon, Enrique; Melnick, Steven

    2014-01-01

    Ethnobotanical evidence suggests that herbs such as brahmi (Bacopa monnieri) and rosemary (Rosmarinus officinalis) may possess anti-oxidant and neuroprotective properties. We compared the anti-oxidant and neuroprotective effects of supercritical extract of Bacopa monnieri (BM) and rosemary anti-oxidant (RA) extract obtained from Rosmarinus officinalis as well as their combination to examine the effects on human glial (U-87 MG) and embryonic mouse hypothalamus (EMH) cells. BM, RA and their com...

  20. Antiosteoporotic Activity of Anthraquinones from Morinda officinalis on Osteoblasts and Osteoclasts

    OpenAIRE

    Cheng-Jian Zheng; Yan-Bin Wu; Lian-Na Sun; Lu-Ping Qin; Ting Han; Jin-Zhong Wu; Qiao-Yan Zhang; Lei Jiao

    2009-01-01

    Bioactivity-guided fractionation led to the successful isolation of antiosteoporotic components, i.e. physicion (1), rubiadin-1-methyl ether (2), 2-hydroxy-1-methoxy- anthraquinone (3), 1,2-dihydroxy-3-methylanthraquinone (4), 1,3,8-trihydroxy-2-methoxy- anthraquinone (5), 2-hydroxymethyl-3-hydroxyanthraquinone (6), 2-methoxyanthraquinone (7) and scopoletin (8) from an ethanolic extract of the roots of Morinda officinalis. Compounds 4-8 are isolated for the first time from M. officinalis. Amo...

  1. Study of Salvia Officinalis Hydroethanolic Extract on Serum Thyroid Hormone Levels in Hypothyroid Male Rat

    OpenAIRE

    N. Mirazi; N. Abdolmaleki; Mahmoodi, M

    2013-01-01

    Introduction & Objective: Medicinal plants are widely used throughout the world. Hypothyroid-ism is an important hormonal disease that causes some disorders in body organs. Salvia offi-cinalis has been known as a medicinal plant since ancient times. In this study the Salvia offi-cinalis extract (SOE) effects on thyroid hormones and TSH in hypothyroid rats have been investigated. Materials & Methods: In this experimental study the SOE extract was prepared and 40 male rats were randomly divided...

  2. High LIN28A Expressing Ovarian Cancer Cells Secrete Exosomes That Induce Invasion and Migration in HEK293 Cells.

    Science.gov (United States)

    Enriquez, Vanessa A; Cleys, Ellane R; Da Silveira, Juliano C; Spillman, Monique A; Winger, Quinton A; Bouma, Gerrit J

    2015-01-01

    Epithelial ovarian cancer is the most aggressive and deadly form of ovarian cancer and is the most lethal gynecological malignancy worldwide; therefore, efforts to elucidate the molecular factors that lead to epithelial ovarian cancer are essential to better understand this disease. Recent studies reveal that tumor cells release cell-secreted vesicles called exosomes and these exosomes can transfer RNAs and miRNAs to distant sites, leading to cell transformation and tumor development. The RNA-binding protein LIN28 is a known marker of stem cells and when expressed in cancer, it is associated with poor tumor outcome. We hypothesized that high LIN28 expressing ovarian cancer cells secrete exosomes that can be taken up by nontumor cells and cause changes in gene expression and cell behavior associated with tumor development. IGROV1 cells were found to contain high LIN28A and secrete exosomes that were taken up by HEK293 cells. Moreover, exposure to these IGROV1 secreted exosomes led to significant increases in genes involved in Epithelial-to-Mesenchymal Transition (EMT), induced HEK293 cell invasion and migration. These changes were not observed with exosomes secreted by OV420 cells, which contain no detectable amounts of LIN28A or LIN28B. No evidence was found of LIN28A transfer from IGROV1 exosomes to HEK293 cells.

  3. Mechanisms of Lin28-Mediated miRNA and mRNA Regulation—A Structural and Functional Perspective

    Directory of Open Access Journals (Sweden)

    Udo Heinemann

    2013-08-01

    Full Text Available Lin28 is an essential RNA-binding protein that is ubiquitously expressed in embryonic stem cells. Its physiological function has been linked to the regulation of differentiation, development, and oncogenesis as well as glucose metabolism. Lin28 mediates these pleiotropic functions by inhibiting let-7 miRNA biogenesis and by modulating the translation of target mRNAs. Both activities strongly depend on Lin28’s RNA-binding domains (RBDs, an N-terminal cold-shock domain (CSD and a C-terminal Zn-knuckle domain (ZKD. Recent biochemical and structural studies revealed the mechanisms of how Lin28 controls let-7 biogenesis. Lin28 binds to the terminal loop of pri- and pre-let-7 miRNA and represses their processing by Drosha and Dicer. Several biochemical and structural studies showed that the specificity of this interaction is mainly mediated by the ZKD with a conserved GGAGA or GGAGA-like motif. Further RNA crosslinking and immunoprecipitation coupled to high-throughput sequencing (CLIP-seq studies confirmed this binding motif and uncovered a large number of new mRNA binding sites. Here we review exciting recent progress in our understanding of how Lin28 binds structurally diverse RNAs and fulfills its pleiotropic functions.

  4. Makorin ortholog LEP-2 regulates LIN-28 stability to promote the juvenile-to-adult transition in Caenorhabditis elegans.

    Science.gov (United States)

    Herrera, R Antonio; Kiontke, Karin; Fitch, David H A

    2016-03-01

    The heterochronic genes lin-28, let-7 and lin-41 regulate fundamental developmental transitions in animals, such as stemness versus differentiation and juvenile versus adult states. We identify a new heterochronic gene, lep-2, in Caenorhabditis elegans. Mutations in lep-2 cause a delay in the juvenile-to-adult transition, with adult males retaining pointed, juvenile tail tips, and displaying defective sexual behaviors. In both sexes, lep-2 mutants fail to cease molting or produce an adult cuticle. We find that LEP-2 post-translationally regulates LIN-28 by promoting LIN-28 protein degradation. lep-2 encodes the sole C. elegans ortholog of the Makorin (Mkrn) family of proteins. Like lin-28 and other heterochronic pathway members, vertebrate Mkrns are involved in developmental switches, including the timing of pubertal onset in humans. Based on shared roles, conservation and the interaction between lep-2 and lin-28 shown here, we propose that Mkrns, together with other heterochronic genes, constitute an evolutionarily ancient conserved module regulating switches in development.

  5. Translating Chinese Place Names into English: The Case of Lin Yutang’s Version of Fusheng Liuji

    Directory of Open Access Journals (Sweden)

    Shuangchang Cui

    2016-04-01

    Full Text Available Chapter Four of Fusheng Liuji (Six Chapters of a Floating Life, a unique autobiographical prose work by early Qing-dynasty author Shen Fu (1763-1825, records Shen's travel to many parts of the country and contains a multitude of place names, whose rich cultural connotations pose a great challenge to the translator. Drawing on Javier Franco Aixelá’s taxonomy of translation strategies for treating culture-specific items, this paper attempts to describe the translation strategies adopted by world-renowned writer and translator Lin Yutang to handle these place names. An analysis of twenty-four examples indicates that Lin tended to preserve Chinese culture carried by the place names through employing cultural conservation strategies, including linguistic translation, transliteration, transliteration plus linguistic translation, transliteration plus annotation, and linguistic translation plus annotation. Furthermore, Lin occasionally resorted to such cultural substitution strategies as absolute universalization and domestication. It is concluded that on the whole Lin adopted a source-oriented approach to translation when working on Shen Fu's work. This study sheds fresh light on Lin's selection of translation strategies in the early years of his lifelong career as “a native interpreter of Chinese culture for Western readers”.Keywords: Place Names, Chinese Culture, Lin Yutang, Translation Strategies

  6. Preliminary phytochemical, acute oral toxicity and antihepatotoxic study of roots of Paeonia officinalis Linn

    Institute of Scientific and Technical Information of China (English)

    Feroz Ahmad; Nahida Tabassum

    2013-01-01

    Objective: To carry out a preliminary phytochemical, acute oral toxicity and antihepatotoxic study of the roots of Paeonia officinalis (P. officinalis) L. Methods: Preliminary phytochemical investigation was done as per standard procedures. Acute oral toxicity study was conducted as per OECD 425 guidelines. The antihepatotoxic activity of aqueous extract of root of P. officinalis was evaluated against carbon tetrachloride (CCl4) induced hepatic damage in rats. Aqueous extract of P. officinalis at the dose levels of 100 and 200 mg/kg body weight was administered daily for 14 d in experimental animals. Liver injury was induced chemically, by CCl4 administration (1 mL/kg i.p.). The hepatoprotective activity was assessed using various biochemical parameters like aspartate aminotransferase (AST), alanine aminotransferase (ALT), serum alkaline phosphatase (SALP), total bilirubin and total protein (TP) along with histopathological studies. Result: Phytochemical screening revealed that the roots ofP. officinalis contain alkaloids, tannins, saponins, glycosides, carbohydrates, flavonoids, terpenes, steroids and proteins. The aqueous extract did not cause any mortality up to 2000 mg/kg. In rats that had received the root extract at the dose of 100 and 200 mg/kg, the substantially elevated AST, ALT, SALP, total bilirubin levels were significantly lowered, respectively, in a dose dependent manner, along with CCl4 while TP levels were elevated in these groups. Histopathology revealed regeneration of the livers in extract treated groups while Silymarin treated rats were almost normal. Conclusions: The aqueous extract of P. officinalis is safe and possesses antihepatotoxic potential.

  7. Automatic Test System of LIN Bus Based on LIN Description File%基于网络描述文件的LIN总线自动测试系统

    Institute of Scientific and Technical Information of China (English)

    彭晓珊; 余明扬; 王勇

    2012-01-01

    Aimed at many weaknesses in the LIN bus testing system like weak compatibility,absent malfunction definitions, difficulties in designing the test equipment and low degree of automation,a new LIN bus automatic test system was proposed based on the LIN description file. The system hardware design consists of upper PC and PIC18F6520 mircocotroller. The upper test software analyses and transforms the LIN description file and the data configured by users which can incarnate the action of the LIN bus application layer's into the test sets of the system, realizes the automatic test of the vehicle LIN bus ECU and definitions of the malfunction's frames ID. The system can realize automatical test and malfunction's definitions for different LIN bus systems according to different LIN description file and data configured by users,so it was significant for upgrading the automation design's lever of the vehicle LIN bus system. The system has been applied in LIN bus ECU test process of the Great Wall motor's Harvard models,and has got a good results.%针对LIN总线系统测试中存在的兼容性差、无故障界定、设计测试工装困难以及自动化程度低等弱点,提出了新型的基于网络描述文件的LIN总线自动测试系统,系统硬件设计采用上位机和下位PIC18F6520微控制器结构,上位机测试软件将LIN网络描述文件和体现LIN总线应用层行为的用户配置数据分析并转化为系统测试集,并实现车载LIN型ECU自动测试和故障帧ID标定,系统能根据不同的网络描述文件和用户配置数据实现对不同LIN总线系统的自动测试和故障界定,对提升车载LIN总线系统的自动化设计水平有重要意义.系统已应用于长城汽车部分哈弗车型的LIN总线相关电控单元的测试环节中,运行效果良好.

  8. Officinalioside, a new lignan glucoside from Borago officinalis L.

    Science.gov (United States)

    Samy, Mamdouh Nabil; Hamed, Ashraf Nageeb El-Sayed; Sugimoto, Sachiko; Otsuka, Hideaki; Kamel, Mohamed Salah; Matsunami, Katsuyoshi

    2016-01-01

    A new lignan glucoside, officinalioside (1), was isolated from n-BuOH fraction of the aerial parts of Borago officinalis L., together with four known compounds: actinidioionoside (2), roseoside (3), crotalionoside C (4) and kaempferol 3-O-β-D-galactopyranoside (5). The structure of the new compound was established by means of spectroscopic and chemical analyses. Compounds 1 and 2 showed a moderate DPPH radical scavenging activity (IC50: 52.6 ± 1.70 and 41.3 ± 0.25 μM, respectively) comparable with that of the standard trolox (16.6 ± 2.2 μM) without any significant cytotoxicity towards human cell line A549 (IC50 > 100 μM). PMID:26382913

  9. [Anthraquinones isolated from Morinda officinalis and Damnacanthus indicus].

    Science.gov (United States)

    Yang, Y J; Shu, H Y; Min, Z D

    1992-01-01

    From chloroform extract of the root of Morinda officinalis, eight anthraquinones were isolated whose structures were deduced to be rubiadin (I), rubiadin-1-methyl ether (II), 1-hydroxyanthraquinone (III), 1-hydroxy-2-methylanthraquinone (IV), 1,6-dihydroxy-2,4-dimethoxyanthraquinone (V), 1,6-dihydroxy-2-methoxyanthraquinone (VI), 1-hydroxy-2-methoxyanthraquinone (VII) and physcion (VIII). Except for compound I and compound II, the other compounds, 1-hydroxy-2-hydroxymethylanthraquinone (IX), 1,3-dihydroxy-2-methoxy-anthraquinone (X), 1,4-dihydroxy-2-methylanthraquinone (XI), 1-methoxy-2-hydroxyanthraquinone (XII) and 1,4-dimethoxy-2-hydroxyanthraquinone (XIII), were isolated from chloroform extract of root of Damnacanthus indicus. Compound V, VI and XIII are new compounds. PMID:1442057

  10. [Effects of primary processing on quality of cortex Magnolia officinalis].

    Science.gov (United States)

    Yu, Shengxian; Zhang, Chunxia; Chen, Chengyu; Yan, Renyi; Yang, Bin; Liao, Chaolin; You, Jinwen

    2010-07-01

    In this paper, the changes of volatile oil and non-volatile ingredients in Cortex Magnolia Officinalis before and after primary processing were determined by an HPLC and a GC-MS method. The method is based on quantitative determination of three index ingredients, beta-eudesmol, honokiol and magnolol, and on qualitative fingerprinting analysis using HPLC and GC. Big differences were observed between processed and unprocessed samples according to their chromatographic fingerprinting data calculated by statistic software. Compared with unprocessed samples, the contents of honokiol and magnolol in processed samples increased, whereas the contents of beta-eudesmol and magnoloside A in processed samples decreased. Magnoloside A was isolated from this plant for the first time. PMID:20939279

  11. LIN7A depletion disrupts cerebral cortex development, contributing to intellectual disability in 12q21-deletion syndrome.

    Directory of Open Access Journals (Sweden)

    Ayumi Matsumoto

    Full Text Available Interstitial deletion of 12q21 has been reported in four cases, which share several common clinical features, including intellectual disability (ID, low-set ears, and minor cardiac abnormalities. Comparative genomic hybridization (CGH analysis using the Agilent Human Genome CGH 180K array was performed with the genomic DNA from a two-year-old Japanese boy with these symptoms, as well as hypoplasia of the corpus callosum. Consequently, a 14 Mb deletion at 12q21.2-q21.33 (nt. 77 203 574-91 264 613 bp, which includes 72 genes, was detected. Of these, we focused on LIN7A, which encodes a scaffold protein that is important for synaptic function, as a possible responsible gene for ID, and we analyzed its role in cerebral cortex development. Western blotting analyses revealed that Lin-7A is expressed on embryonic day (E 13.5, and gradually increases in the mouse brain during the embryonic stage. Biochemical fractionation resulted in the enrichment of Lin-7A in the presynaptic fraction. Suppression of Lin-7A expression by RNAi, using in utero electroporation on E14.5, delayed neuronal migration on postnatal day (P 2, and Lin-7A-deficient neurons remained in the lower zone of the cortical plate and the intermediate zone. In addition, when Lin-7A was silenced in cortical neurons in one hemisphere, axonal growth in the contralateral hemisphere was delayed; development of these neurons was disrupted such that one half did not extend into the contralateral hemisphere after leaving the corpus callosum. Taken together, LIN7A is a candidate gene responsible for 12q21-deletion syndrome, and abnormal neuronal migration and interhemispheric axon development may contribute to ID and corpus callosum hypoplasia, respectively.

  12. Stand establishment and yield potential of organically grown seeded and transplanted medicinal herbs

    Science.gov (United States)

    Field studies were conducted at Las Cruces, N.M., and Alcalde, N.M., to compare direct seeding to transplanting for stand establishment and yield estimates of calendula (Calendula officinalis L.), catnip (Nepeta cataria L.), lemon balm (Melissa officinalis L.), stinging nettles (Urtica dioica L.), a...

  13. The stardust family protein MPP7 forms a tripartite complex with LIN7 and DLG1 that regulates the stability and localization of DLG1 to cell junctions.

    Science.gov (United States)

    Bohl, Joanna; Brimer, Nicole; Lyons, Charles; Vande Pol, Scott B

    2007-03-30

    MPP7, a previously uncharacterized member of the p55 Stardust family of membrane-associated guanylate kinase (MAGUK) proteins, was found in a tripartite complex with DLG1 and LIN7A or LIN7C. MPP7 dimerizes with all three LIN7 family members (LIN7A, -B, and -C) through interaction of the single L27 domain of LIN7 with the carboxyl-terminal L27 domain of MPP7, thereby stabilizing both proteins. The dimer of MPP7 with LIN7A or LIN7C associates with DLG1 through an interaction requiring the amino-terminal L27 domain of MPP7. The amino-terminal L27 domain of MPP7 is not sufficient for interaction with DLG1 but interacts efficiently only if MPP7 is in a complex with LIN7A or -C. Thus the specificity of interaction of DLG1 with the LIN7-MPP7 complex is determined by L27 interactions with both MPP7 and LIN7. The tripartite complex forms in a ratio of 1:1:1 and localizes to epithelial adherens junctions in a manner dependent upon MPP7. Expression of MPP7 stabilizes DLG1 in an insoluble compartment. Expression of MPP7 deleted of the PDZ or Src homology 3 domain redistributes MPP7, DLG1, and LIN7 out of adherens junctions and into the soluble cytoplasmic fraction without changing the localization of E-cadherin. Thus, the stability and localization of DLG1 to cell-cell junctions are complex functions determined by the expression and association of particular Stardust family members together with particular LIN7 family members.

  14. Investigations on lin-Benzopurines With Respect to Dissociation Behavior, Pocket Cross-Talk, Targeting Resistance Mutants, Residual Mobility, and Scaffold Optimization

    OpenAIRE

    Neeb, Manuel

    2014-01-01

    The present thesis deals with the characterization and improvement of selective antibiotics targeting the enzyme tRNA–guanine transglycosylase. Recently, the lin-benzopurine scaffold was introduced as promising starting point for the structure-based design of TGT inhibitors. Two classes, namely the lin-benzoguanines and lin-benzohypoxanthines, were found to inhibit the target enzyme in the nanomolar range. However, the analyzed ...

  15. Overexpression of Lin28 Decreases the Chemosensitivity of Gastric Cancer Cells to Oxaliplatin, Paclitaxel, Doxorubicin, and Fluorouracil in Part via microRNA-107.

    Directory of Open Access Journals (Sweden)

    Rongyue Teng

    Full Text Available Higher Lin28 expression is associated with worse pathologic tumor responses in locally advanced gastric cancer patients undergoing neoadjuvant chemotherapy. However, the characteristics of Lin28 and its mechanism of action in chemotherapy resistance is still unclear. In this study, we found that transfection of Lin28 into gastric cancer cells (MKN45 and MKN28 increased their resistance to the chemo-drugs oxaliplatin (OXA, paclitaxel (PTX, doxorubicin (ADM, and fluorouracil (5-Fu compared with gastric cancer cells transfected with a control vector. When knockdown Lin28 expression by Lin28 small interfering RNA (siRNA was evaluated in vitro, we found that the resistance to chemo-drugs was reduced. Furthermore, we found that Lin28 up-regulates C-myc and P-gp and down-regulates Cylin D1. Finally, we found that miR-107 is a target microRNA of Lin28 and that it participates in the mechanism of chemotherapy resistance. Our results suggest that the Lin28/miR-107 pathway could be one of many signaling pathways regulated by Lin28 and associated with gastric cancer chemo-resistance.

  16. Interaction sol-structure non-linéaire en analyse sismique

    OpenAIRE

    Nieto Ferro, Alex

    2013-01-01

    Ce travail détaille une approche de calcul pour la résolution de problèmes dynamiques qui combinent des discrétisations en temps et dans le domaine de Laplace reposant sur une technique de sous-structuration. En particulier, la méthode développée cherche à remplir le besoin industriel de réaliser des calculs dynamiques tridimensionnels pour le risque sismique en prenant en compte des effets non-linéaires d'interaction sol-structure (ISS). Deux sous-domaines sont considérés dans ce problème. D...

  17. Guides polymères pour fonctions optiques non linéaires

    OpenAIRE

    Mahé, Hind; Bosc, Dominique; Hardy, Isabelle; Fontanieu, Lénaïck; Leroy, Etienne

    2010-01-01

    Certaines molécules organiques présentent d'importantes non linéarités optique, avec des temps de réponses compatibles avec le haut débit pour des fonctions de traitement du signal optique. La difficulté réside dans la mise en œuvre technologique de ces matériaux. Nous résumerons les travaux et résultats obtenus au CCLO dans ce domaine.

  18. Nucleotide sequence and taxonomical distribution of the bacteriocin gene lin cloned from Brevibacterium linens M18.

    OpenAIRE

    Valdes-Stauber, N; Scherer, S

    1996-01-01

    Linocin M18 is an antilisterial bacteriocin produced by the red smear cheese bacterium Brevibacterium linens M18. Oligonucleotide probes based on the N-terminal amino acid sequence were used to locate its single copy gene, lin, on the chromosomal DNA. The amino acid composition, N-terminal sequence, and molecular mass derived from the nucleotide sequence of an open reading frame of 798 nucleotides coding for 266 amino acids found on a 3-kb BamHI restriction fragment correspond closely to thos...

  19. LIN28B polymorphisms are associated with central precocious puberty and early puberty in girls

    Directory of Open Access Journals (Sweden)

    Sung Won Park

    2012-10-01

    Full Text Available &lt;B&gt;Purpose:&lt;/B&gt; Single-nucleotide polymorphism (SNP markers within &lt;i&gt;LIN28B&lt;/i&gt; have been reported to be related to the timing of pubertal growth. However, no study has investigated the frequency of genetic markers in girls with precocious puberty (PP or early puberty (EP. This study aimed to determine the frequency of putative genetic markers in girls with PP or EP. &lt;B&gt;Methods:&lt;/B&gt; Genomic DNAs were obtained from 77 and 109 girls that fulfilled the criteria for PP and EP, respectively. The controls in this study were 144 healthy volunteers between 20 and 30 years of age. The haplotypes were reconstructed using 11 SNPs of &lt;i&gt;LIN28B&lt;/i&gt;, and haplotype association analysis was performed. The haplotype frequencies were compared. Differences in the clinical and laboratory parameters were analyzed according to the haplotype dosage. &lt;B&gt;Results:&lt;/B&gt; Eleven SNPs in &lt;i&gt;LIN28B&lt;/i&gt; were all located in a block that was in linkage disequilibrium. The haplotype could be reconstructed using 2 representative SNPs, rs4946651 and rs369065. The AC haplotype was less frequently observed in the PP group than in the controls (0.069 vs. 0.144, &lt;I&gt;P&lt;/I&gt;=0.010. The trend that girls with non-AC haplotypes tended to have earlier puberty onset (&lt;I&gt;P&lt;/I&gt;=0.037 was illustrated even in the EP+PP patient group by Kaplan-Meier analysis. &lt;B&gt;Conclusion:&lt;/B&gt; The results of the present study showed that non-AC haplotypes of &lt;i&gt;LIN28B&lt;/i&gt;had a significant association with PP in girls.

  20. The Study on Lin Yutang’s Translation from the Perspective of Aesthetics

    Institute of Scientific and Technical Information of China (English)

    瞿宁

    2013-01-01

    Since the main purposes of translation lies in expressing to the target readers both the message and the aesthetic value of the source text and making the readers ideologically inspired and aesthetically entertained, Lin Yutang conclusively sum up that the aesthetic criteria in translation should be faithfulness, expressiveness and beauty throughout his translation practices. This thesis mainly focus on the aesthetic criteria of Lin’s translation as well as the aesthetic reproduction in his translation which illustrates the unity reached in Lin’s translation in terms of both the aesthetic features and the loyalty to the meaning.

  1. Genetic variation in LIN28B is associated with the timing of puberty

    OpenAIRE

    Ong, Ken K.; Elks, Cathy E.; Li, Shengxu; Zhao, Jing Hua; Luan, Jian'an; Andersen, Lars B.; Bingham, Sheila A; Brage, Soren; Smith, George Davey; Ekelund, Ulf; Gillson, Christopher J; Glaser, Beate; Golding, Jean; Hardy, Rebecca; Khaw, Kay-Tee

    2009-01-01

    The timing of puberty is highly variable1. We carried out a genome-wide association study for age at menarche in 4,714 women and report an association in LIN28B on chromosome 6 (rs314276, minor allele frequency (MAF) = 0.33, P = 1.5 × 10−8). In independent replication studies in 16,373 women, each major allele was associated with 0.12 years earlier menarche (95% CI = 0.08–0.16; P = 2.8 × 10−10; combined P = 3.6 × 10−16). This allele was also associated with earlier breast development in girls...

  2. LIN Bus and Its Application%LIN总线及其应用

    Institute of Scientific and Technical Information of China (English)

    侯志华

    2009-01-01

    LIN(Local Interconnect Network)是由汽车厂商专门为汽车开发的一种低成本串行通信网络,用于实现汽车中的分布式电子系统控制.本文介绍了LIN总线的基本特征、网络结构及其在汽车上的应用情况,凸显了汽车网络系统低端使用LIN的必要性和优越性.

  3. LIN总线控制器的FPGA设计%The FPGA realization of LIN Bus controller

    Institute of Scientific and Technical Information of China (English)

    肖木; 王丽芳; 唐晓泉

    2007-01-01

    LIN(Local Interconnect Network)总线是一种新型低成本汽车车身网络低端通讯总线.本文针对LIN总线协议的特点,设计了基于ALTERA芯片EP1K50TC144-3的LIN总线控制器.该总线控制器能够实现LIN总线发送与接收,并具有LIN总线自动唤醒与自同步功能.

  4. Genome packaging in EL and Lin68, two giant phiKZ-like bacteriophages of P. aeruginosa

    Energy Technology Data Exchange (ETDEWEB)

    Sokolova, O.S., E-mail: sokolova@mail.bio.msu.ru [M.V. Lomonosov Moscow State University, Moscow (Russian Federation); A.V. Shoubnikov Institute of Crystallography RAS, Moscow (Russian Federation); Shaburova, O.V. [I.I. Mechnikov Research Institute of Vaccines and Sera, RAMS, Moscow (Russian Federation); Pechnikova, E.V. [A.V. Shoubnikov Institute of Crystallography RAS, Moscow (Russian Federation); Shaytan, A.K. [M.V. Lomonosov Moscow State University, Moscow (Russian Federation); Krylov, S.V. [I.I. Mechnikov Research Institute of Vaccines and Sera, RAMS, Moscow (Russian Federation); Kiselev, N.A. [A.V. Shoubnikov Institute of Crystallography RAS, Moscow (Russian Federation); Krylov, V.N. [I.I. Mechnikov Research Institute of Vaccines and Sera, RAMS, Moscow (Russian Federation)

    2014-11-15

    A unique feature of the Pseudomonas aeruginosa giant phage phiKZ is its way of genome packaging onto a spool-like protein structure, the inner body. Until recently, no similar structures have been detected in other phages. We have studied DNA packaging in P. aeruginosa phages EL and Lin68 using cryo-electron microscopy and revealed the presence of inner bodies. The shape and positioning of the inner body and the density of the DNA packaging in EL are different from those found in phiKZ and Lin68. This internal organization explains how the shorter EL genome is packed into a large EL capsid, which has the same external dimensions as the capsids of phiKZ and Lin68. The similarity in the structural organization in EL and other phiKZ-like phages indicates that EL is phylogenetically related to other phiKZ-like phages, and, despite the lack of detectable DNA homology, EL, phiKZ, and Lin68 descend from a common ancestor. - Highlights: • We performed a comparative structural study of giant P. aeruginosa phages: EL, Lin68 and phiKZ. • We revealed that the inner body is a common feature in giant phages. • The phage genome size correlates with the overall dimensions of the inner body.

  5. Genome packaging in EL and Lin68, two giant phiKZ-like bacteriophages of P. aeruginosa

    International Nuclear Information System (INIS)

    A unique feature of the Pseudomonas aeruginosa giant phage phiKZ is its way of genome packaging onto a spool-like protein structure, the inner body. Until recently, no similar structures have been detected in other phages. We have studied DNA packaging in P. aeruginosa phages EL and Lin68 using cryo-electron microscopy and revealed the presence of inner bodies. The shape and positioning of the inner body and the density of the DNA packaging in EL are different from those found in phiKZ and Lin68. This internal organization explains how the shorter EL genome is packed into a large EL capsid, which has the same external dimensions as the capsids of phiKZ and Lin68. The similarity in the structural organization in EL and other phiKZ-like phages indicates that EL is phylogenetically related to other phiKZ-like phages, and, despite the lack of detectable DNA homology, EL, phiKZ, and Lin68 descend from a common ancestor. - Highlights: • We performed a comparative structural study of giant P. aeruginosa phages: EL, Lin68 and phiKZ. • We revealed that the inner body is a common feature in giant phages. • The phage genome size correlates with the overall dimensions of the inner body

  6. Chemical composition of Rosmarinus officinalis essential oil and antioxidant action against gastric damage induced by absolute ethanol in the rat

    Institute of Scientific and Technical Information of China (English)

    Christiane Takayama; Anderson Luiz-Ferreira; Felipe Meira de-Faria; Ana Cristina Alves de Almeida; Ricardo Jose Dunder; Luis Paulo Manzo; Eduardo Augusto Rabelo Socca; Leonia Maria Batista; Marcos Jos e Salvador

    2016-01-01

    Objective: To evaluate the antioxidant activity of the essential oil obtained from Ros-marinus officinalis (R. officinalis) in ethanol-induced gastric ulcer model in vivo. Methods: The antioxidant properties of the essential oil obtained from R. officinalis were evaluated against gastric injury induced by absolute ethanol. Gastric tissues were pre-pared to enzymatic assays. The levels of glutathione, lipid peroxides, and the activities of glutathione peroxidase, superoxide dismutase and myeloperoxidase were measured. Results: Ethanol produced severe hemorrhagic lesions in the stomach with ulcerative lesion of (140.2 ± 37.2) mm2. In animals pretreated with essential oil of R. officinalis (50 mg/kg, p.o.), a significant inhibition of mucosal injury of (21.2 ± 7.1) mm2 (84%inhibition) was observed. The essential oil of R. officinalis protected the gastric mucosa probably by modulating the activities of the enzymes (superoxide dismutase and gluta-thione peroxidase) and increasing or maintaining the levels of glutathione. In addition, lipid peroxides levels were reduced. The essential oil of R. officinalis was analyzed by gas chromatography-mass spectrometer and the main constituents were cineole (28.5%), camphor (27.7%) and alpha-pinene (21.3%). Conclusions: We suggest that the monoterpenes present in the essential oil obtained from R. officinalis may be among the active principles responsible for the antioxidant activity shown by essential oil of R. officinalis.

  7. Brachycorynella asparagi (Mordv. Induced—Oxidative Stress and Antioxidative Defenses of Asparagus officinalis L.

    Directory of Open Access Journals (Sweden)

    Beata Borowiak-Sobkowiak

    2016-10-01

    Full Text Available The aim of this study was to investigate whether and to what extent oxidative stress is induced in leaves of one- and two-month-old plants of Asparagus officinalis L. cv. Argenteuil infested by Brachycorynella asparagi (Mordvilko at a varied population size. The pest B. asparagi has been described as the most damaging species feeding on asparagus. Analyses using electron paramagnetic resonance (EPR demonstrated generally higher concentrations of semiquinone radicals with g-values of 2.0045 ± 0.0005 and 2.0026 ± 0.0005 in Asparagus officinalis (A. officinalis leaves after Brachycorynella asparagi (B. asparagi infestation than in the control. Observations of leaves under a confocal microscope showed a post-infestation enhanced generation of the superoxide anion radical (O2•− and hydrogen peroxide (H2O2 in comparison to the control. Strong fluctuations in Mn2+ ion levels detected by EPR spectroscopy versus time were detected in leaves infested by aphids, which may indicate the involvement of these ions in the control of O2•− production. An enhanced superoxide dismutase activity is an important element in leaf defense against oxidative stress. Visible symptoms were found in aphid-infested A. officinalis. Damage to leaves of one- and two-month-old A. officinalis plants by the aphid B. asparagi was dependent on the intensity, duration of infestation and plant age.

  8. Brachycorynella asparagi (Mordv.) Induced—Oxidative Stress and Antioxidative Defenses of Asparagus officinalis L.

    Science.gov (United States)

    Borowiak-Sobkowiak, Beata; Woźniak, Agnieszka; Bednarski, Waldemar; Formela, Magda; Samardakiewicz, Sławomir; Morkunas, Iwona

    2016-01-01

    The aim of this study was to investigate whether and to what extent oxidative stress is induced in leaves of one- and two-month-old plants of Asparagus officinalis L. cv. Argenteuil infested by Brachycorynella asparagi (Mordvilko) at a varied population size. The pest B. asparagi has been described as the most damaging species feeding on asparagus. Analyses using electron paramagnetic resonance (EPR) demonstrated generally higher concentrations of semiquinone radicals with g-values of 2.0045 ± 0.0005 and 2.0026 ± 0.0005 in Asparagus officinalis (A. officinalis) leaves after Brachycorynella asparagi (B. asparagi) infestation than in the control. Observations of leaves under a confocal microscope showed a post-infestation enhanced generation of the superoxide anion radical (O2•−) and hydrogen peroxide (H2O2) in comparison to the control. Strong fluctuations in Mn2+ ion levels detected by EPR spectroscopy versus time were detected in leaves infested by aphids, which may indicate the involvement of these ions in the control of O2•− production. An enhanced superoxide dismutase activity is an important element in leaf defense against oxidative stress. Visible symptoms were found in aphid-infested A. officinalis. Damage to leaves of one- and two-month-old A. officinalis plants by the aphid B. asparagi was dependent on the intensity, duration of infestation and plant age. PMID:27775613

  9. Preliminary assessment of trace elements effects on essential oil production of Melissa officinalis L. (Lemon balm)

    Energy Technology Data Exchange (ETDEWEB)

    Sussa, Fabio V.; Duarte, Celina L.; Andrade, Luana S.; Silva, Paulo S.C., E-mail: pscsilva@ipen.br, E-mail: clduarte@ipen.br, E-mail: fabiosussa@uol.com.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Furlan, Marcos R., E-mail: furlanagro@gmail.com [Universidade de Taubate, SP (Brazil). Departamento de Ciencias Agrarias

    2013-07-01

    Melissa officinalis L., Lemon balm, (Lamiaceae) is an herb used as medicine, condiment and in the cosmetic and perfumery industry due to its essential oil. In this study a preliminary assessment of trace elements effects on essential oil production was performed in order to verify an improvement in its quality. The Melissa officinalis samples were harvested from three different soil sites localized in Ibirapuera Park, São Paulo, Brazil. Elemental concentration for the elements As, Ba, Br, Ce, Cl, Co, Cr, Cs, Eu, Fe, Hf, K, La, Lu, Mg, Mn, Na, Nd, Rb, Sb, Sc, Se, Sm, Ta, Tb, Ti, Th, U, V, Yb, Zn and Zr was determined by Instrumental Neutron Activation Analysis (INAA) in Melissa officinalis leaves and surrounding soil. The essential oil was extracted from its leaves by hydrodistillation process in Clevenger apparatus and analyzed by gas chromatography coupled to a mass spectrometer (GC-MS). Certified reference materials NIST SRM-1515 Apple Leaves, NIST SRM-1547 Peach Leaves and NIST SRM-1573a Tomato Leaves were analyzed for quality control. Our results showed Geranial and Neral were identified as the major compounds in the essential oil extracted from Melissa officinalis L. for all collected sites. However, the relative proportion of some chemical constituents was altered according to the site collected. The preliminary results showed that the production of essential oil by Melissa officinalis must be positively correlated with the concentrations of Rb, Zn and negatively correlated with Sc, Mn, La, K, Fe, and Cr. (author)

  10. Cancer Prevention and Health Benefices of Traditionally Consumed Borago officinalis Plants.

    Science.gov (United States)

    Lozano-Baena, María-Dolores; Tasset, Inmaculada; Muñoz-Serrano, Andrés; Alonso-Moraga, Ángeles; de Haro-Bailón, Antonio

    2016-01-01

    Nowadays, healthy eating is increasing the demand of functional foods by societies as sources of bioactive products with healthy qualities. For this reason, we tested the safety of the consumption of Borago officinalis L. and its main phenolic components as well as the possibility of its use as a nutraceutical plant to help in cancer prevention. The in vivo Drosophila Somatic Mutation and Recombination Test (SMART) and in vitro HL-60 human cell systems were performed, as well-recognized methods for testing genotoxicity/cytotoxicity of bioactive compounds and plant products. B. officinalis and the tested compounds possess antigenotoxic activity. Moreover, B. officinalis wild type cultivar exerts the most antigenotoxic values. Cytotoxic effect was probed for both cultivars with IC50 values of 0.49 and 0.28 mg · mL(-1) for wild type and cultivated plants respectively, as well as their constituent rosmarinic acid and the assayed phenolic mixture (IC50 = 0.07 and 0.04 mM respectively). B. officinalis exerts DNA protection and anticarcinogenic effects as do its component rosmarinic acid and the mixture of the main phenolics presented in the plant. In conclusion, the results showed that B. officinalis may represent a high value plant for pleiotropic uses and support its consumption as a nutraceutical plant. PMID:26797631

  11. Cancer Prevention and Health Benefices of Traditionally Consumed Borago officinalis Plants

    Directory of Open Access Journals (Sweden)

    María-Dolores Lozano-Baena

    2016-01-01

    Full Text Available Nowadays, healthy eating is increasing the demand of functional foods by societies as sources of bioactive products with healthy qualities. For this reason, we tested the safety of the consumption of Borago officinalis L. and its main phenolic components as well as the possibility of its use as a nutraceutical plant to help in cancer prevention. The in vivo Drosophila Somatic Mutation and Recombination Test (SMART and in vitro HL-60 human cell systems were performed, as well-recognized methods for testing genotoxicity/cytotoxicity of bioactive compounds and plant products. B. officinalis and the tested compounds possess antigenotoxic activity. Moreover, B. officinalis wild type cultivar exerts the most antigenotoxic values. Cytotoxic effect was probed for both cultivars with IC50 values of 0.49 and 0.28 mg·mL−1 for wild type and cultivated plants respectively, as well as their constituent rosmarinic acid and the assayed phenolic mixture (IC50 = 0.07 and 0.04 mM respectively. B. officinalis exerts DNA protection and anticarcinogenic effects as do its component rosmarinic acid and the mixture of the main phenolics presented in the plant. In conclusion, the results showed that B. officinalis may represent a high value plant for pleiotropic uses and support its consumption as a nutraceutical plant.

  12. Emblica officinalis improves glycemic status and oxidative stress in STZ induced type 2 diabetic model rats

    Institute of Scientific and Technical Information of China (English)

    Aneesa Ansari; Md Shahed Zaman Shahriar; Md Mehedi Hassan; Shukla Rani Das; Begum Rokeya; Md Anwarul Haque; Md Enamul Haque; Nirupam Biswas; Tama Sarkar

    2014-01-01

    Objective: To evaluate the antidiabetic and antioxidant potential of Emblica officinalis (E. officinalis) fruit on normal and type 2 diabetic rats. Methods: Type 2 diabetes was induced into the male Long-Evans rats. The rats were divided into nine groups including control groups receiving water, type 2 diabetic controls, type 2 diabetic rats treated with glibenclamide (T2GT) and type 2 diabetic rats treated with aqueous extract of fruit pulp of E. officinalis. They were fed orally for 8 weeks with a single feeding. Blood was collected by cutting the tail tip on 0 and 28 days and by decapitation on 56 day. Packed red blood cells and serum were used for evaluating different biochemical parameters. Results: Four weeks administration of aqueous extract of E. officinalis improved oral glucose tolerance in type 2 rats and after 8 weeks it caused significant (P<0.007) reduction in fasting serum glucose level compared to 0 day. Triglycerides decreased by 14% but there was no significant change in serum ALT, creatinine, cholesterol and insulin level in any group. Furthermore, reduced erythrocyte malondialdehyde level showed no significant change (P<0.07) but reduced glutathione content was found to be increased significantly (P<0.05).Conclusions:The aqueous extract of E. officinalis has a promising antidiabetic and antioxidant properties and may be considered for further clinical studies in drug development.

  13. Variation of essential oil composition of Melissa officinalis L. leaves during different stages of plant growth

    Institute of Scientific and Technical Information of China (English)

    Keivan Saeb; Sara Gholamrezaee

    2012-01-01

    Objective: To determine the best time of harvest for Melissa officinalis (M. officinalis) L. to gain highest amounts essential oil.Methods: M. officinalis leaves were harvested in three different stages (before flowering stage, flowering stage and after of flowering stage) and were dried. The essential oils were isolated by hydro- distillation and analyzed by GC/MS.Results: It showed that most essential oils of plants were in before flowering stage. In before flowering stage 37 compounds were identified in leaves oil of M.officinalis. The major components before flowering stage were decadienal (29.38%), geraniol (25.3%), caryophyllene oxide (8.75%), geranyl acetate (5.41%). In the flowering stage 36 compounds were identified as the major components of plant essential oils: decadienal (28.04%), geraniol (24.97%), caryophyllene oxide (7.55%), caryophyllene E (4.65%) and 16 components in the after flowering stage of plant were identified as the major components carvacrol (37.62%), methyl citronellate (32.34%), geranyl acetate (5.82%), caryophyllene (5.50%).Conclusions: The essential oils yields vary considerably from month-to-month and is also influenced by the micro-environment (sun or shade) in which the plant is growing. We found that the essential oil content of M. officinalis L. of leaves is significantly affected by harvesting stages.

  14. Drought-tolerant rice germplasm developed from an Oryza officinalis transformation-competent artificial chromosome clone.

    Science.gov (United States)

    Liu, R; Zhang, H H; Chen, Z X; Shahid, M Q; Fu, X L; Liu, X D

    2015-10-29

    Oryza officinalis has proven to be a natural gene reservoir for the improvement of domesticated rice as it carries many desirable traits; however, the transfer of elite genes to cultivated rice by conventional hybridization has been a challenge for rice breeders. In this study, the conserved sequence of plant stress-related NAC transcription factors was selected as a probe to screen the O. officinalis genomic transformation-competent artificial chromosome library by Southern blot; 11 positive transformation-competent artificial chromosome clones were subsequently detected. By Agrobacterium-mediated transformation, an indica rice variety, Huajingxian 74 (HJX74), was transformed with a TAC clone harboring a NAC gene-positive genomic fragment from O. officinalis. Molecular analysis revealed that the O. officinalis genomic fragment was integrated into the genome of HJX74. The transgenic lines exhibited high tolerance to drought stress. Our results demonstrate that the introduction of stress-related transformation-competent artificial chromosome clones, coupled with a transgenic validation approach, is an effective method of transferring agronomically important genes from O. officinalis to cultivated rice.

  15. Cancer Prevention and Health Benefices of Traditionally Consumed Borago officinalis Plants.

    Science.gov (United States)

    Lozano-Baena, María-Dolores; Tasset, Inmaculada; Muñoz-Serrano, Andrés; Alonso-Moraga, Ángeles; de Haro-Bailón, Antonio

    2016-01-01

    Nowadays, healthy eating is increasing the demand of functional foods by societies as sources of bioactive products with healthy qualities. For this reason, we tested the safety of the consumption of Borago officinalis L. and its main phenolic components as well as the possibility of its use as a nutraceutical plant to help in cancer prevention. The in vivo Drosophila Somatic Mutation and Recombination Test (SMART) and in vitro HL-60 human cell systems were performed, as well-recognized methods for testing genotoxicity/cytotoxicity of bioactive compounds and plant products. B. officinalis and the tested compounds possess antigenotoxic activity. Moreover, B. officinalis wild type cultivar exerts the most antigenotoxic values. Cytotoxic effect was probed for both cultivars with IC50 values of 0.49 and 0.28 mg · mL(-1) for wild type and cultivated plants respectively, as well as their constituent rosmarinic acid and the assayed phenolic mixture (IC50 = 0.07 and 0.04 mM respectively). B. officinalis exerts DNA protection and anticarcinogenic effects as do its component rosmarinic acid and the mixture of the main phenolics presented in the plant. In conclusion, the results showed that B. officinalis may represent a high value plant for pleiotropic uses and support its consumption as a nutraceutical plant.

  16. Preliminary assessment of trace elements effects on essential oil production of Melissa officinalis L. (Lemon balm)

    International Nuclear Information System (INIS)

    Melissa officinalis L., Lemon balm, (Lamiaceae) is an herb used as medicine, condiment and in the cosmetic and perfumery industry due to its essential oil. In this study a preliminary assessment of trace elements effects on essential oil production was performed in order to verify an improvement in its quality. The Melissa officinalis samples were harvested from three different soil sites localized in Ibirapuera Park, São Paulo, Brazil. Elemental concentration for the elements As, Ba, Br, Ce, Cl, Co, Cr, Cs, Eu, Fe, Hf, K, La, Lu, Mg, Mn, Na, Nd, Rb, Sb, Sc, Se, Sm, Ta, Tb, Ti, Th, U, V, Yb, Zn and Zr was determined by Instrumental Neutron Activation Analysis (INAA) in Melissa officinalis leaves and surrounding soil. The essential oil was extracted from its leaves by hydrodistillation process in Clevenger apparatus and analyzed by gas chromatography coupled to a mass spectrometer (GC-MS). Certified reference materials NIST SRM-1515 Apple Leaves, NIST SRM-1547 Peach Leaves and NIST SRM-1573a Tomato Leaves were analyzed for quality control. Our results showed Geranial and Neral were identified as the major compounds in the essential oil extracted from Melissa officinalis L. for all collected sites. However, the relative proportion of some chemical constituents was altered according to the site collected. The preliminary results showed that the production of essential oil by Melissa officinalis must be positively correlated with the concentrations of Rb, Zn and negatively correlated with Sc, Mn, La, K, Fe, and Cr. (author)

  17. Kinetic and sequence-structure-function analysis of LinB enzyme variants with β- and δ-hexachlorocyclohexane.

    Directory of Open Access Journals (Sweden)

    Rinku Pandey

    Full Text Available Organochlorine insecticide hexachlorocyclohexane (HCH has recently been classified as a 'Persistent Organic pollutant' by the Stockholm Convention. The LinB haloalkane dehalogenase is a key upstream enzyme in the recently evolved Lin pathway for the catabolism of HCH in bacteria. Here we report a sequence-structure-function analysis of ten naturally occurring and thirteen synthetic mutants of LinB. One of the synthetic mutants was found to have ∼80 fold more activity for β- and δ-hexachlorocyclohexane. Based on detailed biophysical calculations, molecular dynamics and ensemble docking calculations, we propose that the latter variant is more active because of alterations to the shape of its active site and increased conformational plasticity.

  18. Polyploid evolution in Oryza officinalis complex of the genus Oryza

    Directory of Open Access Journals (Sweden)

    Li Changbao

    2009-10-01

    Full Text Available Abstract Background Polyploidization is a prominent process in plant evolution, whereas the mechanism and tempo-spatial process remained poorly understood. Oryza officinalis complex, a polyploid complex in the genus Oryza, could exemplify the issues not only for it covering a variety of ploidy levels, but also for the pantropical geographic pattern of its polyploids in Asia, Africa, Australia and Americas, in which a pivotal genome, the C-genome, witnessed all the polyploidization process. Results Tracing the C-genome evolutionary history in Oryza officinalis complex, this study revealed the genomic relationships, polyploid forming and diverging times, and diploidization process, based on phylogeny, molecular-clock analyses and fluorescent in situ hybridization using genome-specific probes. Results showed that C-genome split with B-genome at ca. 4.8 Mya, followed by a series of speciation of C-genome diploids (ca. 1.8-0.9 Mya, which then partook in successive polyploidization events, forming CCDD tetraploids in ca. 0.9 Mya, and stepwise forming BBCC tetraploids between ca. 0.3-0.6 Mya. Inter-genomic translocations between B- and C-genomes were identified in BBCC tetraploid, O. punctata. Distinct FISH (fluorescent in situ hybridization patterns among three CCDD species were visualized by C-genome-specific probes. B-genome was modified before forming the BBCC tetraploid, O. malampuzhaensis. Conclusion C-genome, shared by all polyploid species in the complex, had experienced different evolutionary history particularly after polyploidization, e.g., inter-genomic exchange in BBCC and genomic invasion in CCDD tetraploids. It diverged from B-genome at 4.8 Mya, then participated in the tetraploid formation spanning from 0.9 to 0.3 Mya, and spread into tropics of the disjunct continents by transcontinentally long-distance dispersal, instead of vicariance, as proposed by this study, given that the continental splitting was much earlier than the C

  19. Wolbachia induces male-specific mortality in the mosquito Culex pipiens (LIN strain.

    Directory of Open Access Journals (Sweden)

    Jason L Rasgon

    Full Text Available BACKGROUND: Wolbachia are maternally inherited endosymbionts that infect a diverse range of invertebrates, including insects, arachnids, crustaceans and filarial nematodes. Wolbachia are responsible for causing diverse reproductive alterations in their invertebrate hosts that maximize their transmission to the next generation. Evolutionary theory suggests that due to maternal inheritance, Wolbachia should evolve toward mutualism in infected females, but strict maternal inheritance means there is no corresponding force to select for Wolbachia strains that are mutualistic in males. METHODOLOGY/PRINCIPAL FINDINGS: Using cohort life-table analysis, we demonstrate that in the mosquito Culex pipiens (LIN strain, Wolbachia-infected females show no fitness costs due to infection. However, Wolbachia induces up to a 30% reduction in male lifespan. CONCLUSIONS/SIGNIFICANCE: These results indicate that the Wolbachia infection of the Culex pipiens LIN strain is virulent in a sex-specific manner. Under laboratory situations where mosquitoes generally mate at young ages, Wolbachia strains that reduce male survival could evolve by drift because increased mortality in older males is not a significant selective force.

  20. 林则徐与科举%Lin Zexu And Imperial Examination

    Institute of Scientific and Technical Information of China (English)

    张媛媛

    2012-01-01

    Lin Zexu plays a decisive role in modern history, and in the imperial examinations, he also occupies a space. As the examination official, he reformed some of the disadvantages in imperial examination, and made some achievements. By the study of the relationship between Lin Zexu and the imperial examination , we can get some enlightenment.%林则徐是中国近代史上举足轻重的人物,在科举史上同样占有一席之地。在家庭以及社会的影响下林则徐走上了科举之路,并最终金榜题名。作为科考试官他公正衡文、选拔真才,并在江南乡试期间改革科场弊端,取得了一定成效。林则徐提倡“经世致用”,并且贯彻于书院教育和科举考试之中,以期士子能关注社会现实,解决社会实际问题。通过考察林则徐与科举的关系,试图从中得到一些启示。

  1. A Novel Method to Increase LinLog CMOS Sensors’ Performance in High Dynamic Range Scenarios

    Directory of Open Access Journals (Sweden)

    Andrés Iborra

    2011-08-01

    Full Text Available Images from high dynamic range (HDR scenes must be obtained with minimum loss of information. For this purpose it is necessary to take full advantage of the quantification levels provided by the CCD/CMOS image sensor. LinLog CMOS sensors satisfy the above demand by offering an adjustable response curve that combines linear and logarithmic responses. This paper presents a novel method to quickly adjust the parameters that control the response curve of a LinLog CMOS image sensor. We propose to use an Adaptive Proportional-Integral-Derivative controller to adjust the exposure time of the sensor, together with control algorithms based on the saturation level and the entropy of the images. With this method the sensor’s maximum dynamic range (120 dB can be used to acquire good quality images from HDR scenes with fast, automatic adaptation to scene conditions. Adaptation to a new scene is rapid, with a sensor response adjustment of less than eight frames when working in real time video mode. At least 67% of the scene entropy can be retained with this method.

  2. zePPeLIN: Distributed Path Planning Using an Overhead Camera Network

    Directory of Open Access Journals (Sweden)

    Andreagiovanni Reina

    2014-08-01

    Full Text Available We introduce zePPeLIN, a distributed system designed to address the challenges of path planning in large, cluttered, dynamic environments. The objective is to define a sequence of instructions to precisely move a ground object (e.g., a mobile robot from an initial to a final configuration in an environment. zePPeLIN is based on a set of wirelessly networked overhead cameras. While each camera only covers a limited environment portion, the camera set fully covers the environment through the union of its fields of view. Path planning is performed in a fully distributed and cooperative way, based on potential diffusion over local Voronoi skeletons and local message exchanging. Additionally, the control of the moving object is fully distributed: it receives movement instructions from each camera when it enters that camera’s field of view. The overall task is made particularly challenging by intrinsic errors in the overlap in cameras’ fields of view. We study the performance of the system as a function of these errors, as well as its scalability for the size and density of the camera network. We also propose a few heuristics to improve performance and computational and communication efficiency. The reported results include both extensive simulation experiments and validation using a real camera network planning for a two-robot system.

  3. Genetic variation in LIN28B is associated with the timing of puberty.

    Science.gov (United States)

    Ong, Ken K; Elks, Cathy E; Li, Shengxu; Zhao, Jing Hua; Luan, Jian'an; Andersen, Lars B; Bingham, Sheila A; Brage, Soren; Smith, George Davey; Ekelund, Ulf; Gillson, Christopher J; Glaser, Beate; Golding, Jean; Hardy, Rebecca; Khaw, Kay-Tee; Kuh, Diana; Luben, Robert; Marcus, Michele; McGeehin, Michael A; Ness, Andrew R; Northstone, Kate; Ring, Susan M; Rubin, Carol; Sims, Matthew A; Song, Kijoung; Strachan, David P; Vollenweider, Peter; Waeber, Gerard; Waterworth, Dawn M; Wong, Andrew; Deloukas, Panagiotis; Barroso, Inês; Mooser, Vincent; Loos, Ruth J; Wareham, Nicholas J

    2009-06-01

    The timing of puberty is highly variable. We carried out a genome-wide association study for age at menarche in 4,714 women and report an association in LIN28B on chromosome 6 (rs314276, minor allele frequency (MAF) = 0.33, P = 1.5 × 10(-8)). In independent replication studies in 16,373 women, each major allele was associated with 0.12 years earlier menarche (95% CI = 0.08-0.16; P = 2.8 × 10(-10); combined P = 3.6 × 10(-16)). This allele was also associated with earlier breast development in girls (P = 0.001; N = 4,271); earlier voice breaking (P = 0.006, N = 1,026) and more advanced pubic hair development in boys (P = 0.01; N = 4,588); a faster tempo of height growth in girls (P = 0.00008; N = 4,271) and boys (P = 0.03; N = 4,588); and shorter adult height in women (P = 3.6 × 10(-7); N = 17,274) and men (P = 0.006; N = 9,840) in keeping with earlier growth cessation. These studies identify variation in LIN28B, a potent and specific regulator of microRNA processing, as the first genetic determinant regulating the timing of human pubertal growth and development. PMID:19448623

  4. XSHOOTER spectroscopy of the enigmatic planetary nebula Lin49 in the Small Magellanic Cloud

    CERN Document Server

    Otsuka, Masaaki; Leal-Ferreira, M L; Aleman, I; Bernard-Salas, J; Cami, J; Ochsendorf, B; Peeters, E; Scicluna, P

    2016-01-01

    We performed a detailed spectroscopic analysis of the fullerene C60-containing planetary nebula (PN) Lin49 in the Small Magellanic Cloud using XSHOOTER at the ESO VLT and the Spitzer/IRS instruments. We derived nebular abundances for nine elements. We used TLUSTY to derive photospheric parameters for the central star. Lin49 is C-rich and metal-deficient PN (Z~0.0006). The nebular abundances are in good agreement with Asymptotic Giant Branch nucleosynthesis models for stars with initial mass 1.25 Msun and metallicity Z = 0.001. Using the TLUSTY synthetic spectrum of the central star to define the heating and ionising source, we constructed the photoionisation model with CLOUDY that matches the observed spectral energy distribution (SED) and the line fluxes in the UV to far-IR wavelength ranges simultaneously. We could not fit the ~1-5 um SED using a model with 0.005-0.1 um-sized graphite grains and a constant hydrogen density shell owing to the prominent near-IR excess, while at other wavelengths the model fit...

  5. Comparative Embryological Studies on Infertility of Interspecific Hybridizations Between Oryza sativa with Different Ploidy Levels and O.officinalis

    Institute of Scientific and Technical Information of China (English)

    FU Xue-lin; LU Yong-gen; LIU Xiang-dong; LI Jin-quan; ZHAO Xing-juan

    2009-01-01

    As maternal parents, diploid (L202-2x) and autotetraploid (L202-4x) of Oryza sativa cv. L202 were crossed with O. officinalis. Embryo development and fertilization in these two crosses were comparatively studied. There were no mature hybrid seeds obtained because all the hybridized spikelets died 30 days after pollination. The main reasons for no seed set were abnormal fertilization and development of the embryos and endosperms in the interspecific hybrids. There were double- fertilization, egg cell single-fertilization and non-fertilization in these crosses. Although 59.45% and 54.87% of hybrid embryos produced in the crosses of L202-2x/O. officinalis and L202-4x/O. officinalis, respectively, hybrid embryos ceased to develop or degenerated and plenty of free endosperm nuclei were in disaggregating state without developing cellular endosperms three days after pollination. Besides, some embryological differences in these two crosses were found, that is, the rate of double-fertilization and total rate of double- and single-fertilization in L202-2x/O. officinalis were higher than those in L202-4x/O. officinalis. The embryo and endosperm of hybrids developed more slowly, and embryos and free endosperm nuclei were more severely degenerated in L202-4x/O. officinalis than in L202-2x/O. officinalis. Five days after pollination, a few of embryos in L202-2x/O. officinalis developed into pear-shaped ones, however, embryos in L202-4x/O. officinalis were all degenerated. Therefore, it is more difficult to obtain interspecific hybrids by wide crosses between autotetraploid of O. sativa and O. officinalis.

  6. Study on the optimum extraction condition of carotenoids from calendula officinalis L. Flowers%金盏菊花类胡萝卜素提取最佳条件的研究

    Institute of Scientific and Technical Information of China (English)

    胡晓丹; 盛群刚; 谢笔钧

    2001-01-01

    在不同溶剂、提取时间、温度、料液比和次数等条件下,通过正交实验,对金盏菊花类胡萝卜素提取最佳条件进行了研究.结果表明:最佳提取工艺条件是用石油醚-丙酮(1:1,V/V)混合溶剂浸提,料液比为1:25(g/ml),在45℃浸提1 h,反复4次,浸提率可达93.5%.

  7. Optimización de soportes de Colágeno tipo I como un sistema de entrega controlada para un extracto de caléndula (Calendula officinalis).

    OpenAIRE

    Jiménez Cruz, Ronald Andrés

    2014-01-01

    En Colombia existe la necesidad de desarrollar productos de ingeniería de tejidos de costo moderado que sean efectivos, seguros y estables para el tratamiento de pérdidas tejidos y órganos. El grupo de ingeniería de tejidos del departamento de Farmacia de la Universidad Nacional, ha desarrollado soportes de colágeno y tejidos artificiales que al ser usados como injerto en heridas de espesor parcial mejoran la regeneración de tejidos blandos. Una alternativa sencilla y menos costosa de tratami...

  8. 不同浓度钾对金盏菊生长发育的影响%THE EFFECT OF DIFFERENT POTASSIUM CONCENTRATION ON Calendula officinalis PLANTS GROWIH AND DEVELOPMENT

    Institute of Scientific and Technical Information of China (English)

    常丽新

    2000-01-01

    为了给金盏菊的规模化无土栽培提供参考依据,采用日本园式营养液配方,探讨不同浓度钾对金盏菊生长发育的影响,对金盏菊植株叶片数、株高、茎粗、鲜重、干物质重量和开花情况进行了分析.结果表明,营养液中钾浓度为400 mg/L时,最适宜金盏菊的生长发育,金盏菊生长对缺钾比较敏感,但对高浓度钾不太敏感.

  9. Influence of Salt Stress on Germination Characteristics of Celosia Cristata and Calendula officinalis Seeds%NaCl胁迫对鸡冠花和金盏菊种子发芽特性的影响

    Institute of Scientific and Technical Information of China (English)

    贾双双; 高青海; 张雪平; 胡景浪

    2013-01-01

    以鸡冠花、金盏菊两种花卉种子为试验材料,研究不同浓度NaCl溶液(0、50、100、150、200、250mmol/L)胁迫下种子发芽率、相对发芽率、发芽势、主根长、下胚轴长、侧根长、幼芽叶绿素含量及根系活力等相关指标的变化规律.试验结果表明:鸡冠花种子在NaCl浓度≤100 mmol/L时,各指标与对照差异不显著,说明鸡冠花对低浓度盐胁迫有一定适应性,而高浓度的盐胁迫对其有很强抑制作用;随着NaCl浓度升高,金盏菊各项测量指标均受到一定程度抑制.说明不同露地花卉种子在发芽期的耐盐力表现出明显的差异,且鸡冠花耐盐能力显著强于金盏菊.

  10. 金盏菊总皂苷提取及其抗氧化、降血脂作用研究%Extraction of Total Saponins from Calendula Officinalis L and its Effects of Anti-oxidation & Hypolipidemic

    Institute of Scientific and Technical Information of China (English)

    刘丽; 国兴明

    2010-01-01

    通过对金盏菊总皂苷的实验室提取工艺及其抗氧化、降血脂作用研究,结果表明:金盏菊总皂苷较为理想的实验室提取工艺为:提取温度75℃,提取时间55 min,乙醇浓度75%,料液比1:35.在此条件下,总皂苷的提取率为2.01%,总固物中总皂苷含量达到35.4%.金盏菊总皂苷对O2·、·OH皆表现出较好的清除效果,清除率分别达到47.29%和88.42%;小鼠血清中的TC、TC、LDL-C与模型组相比,均有一定程度的下降.

  11. Effect of Calendula officinalis L.Extracts on Performance and Egg Qualities of Laying Hens%金盏菊提取物对蛋鸡生产性能和蛋品质的影响

    Institute of Scientific and Technical Information of China (English)

    高杨; 陈辉; 黄仁录; 王翠菊; 杨秋霞; 王学强

    2010-01-01

    为研究日粮中添加不同水平金盏菊提取物对蛋鸡生产性能和蛋品质的影响,试验选取400日龄海兰灰蛋鸡224只,随机分成4组,每组4个重复,每个重复14只,金盏菊提取物添加水平分别为0、1、2、4 g/kg,试验期14 d.结果:蛋鸡日粮中添加金盏菊提取物对蛋鸡产蛋率、平均蛋重、平均耗料量、料蛋比、蛋壳厚度、蛋壳相对重和蛋黄比率均无显著影响(P>0.05);哈氏单位随着金盏菊提取物添加量的增加逐渐增加,添加剂量为2、4 g/kg试验组与对照组相比差异显著(P<0.05);蛋黄颜色自第2天起,各试验组与对照组相比逐渐加深,试验第5天,2、4g/kg的试验组蛋黄颜色达到显著水平(P<0.05),到第8天达到极显著水平(P<0.01);其中添加金盏菊提取物剂量达到2 g/kg时,蛋黄颜色极显著提高(P<0.01).结果提示,2 g/kg金盏菊提取物为适宜添加量.

  12. 不同浓度氯化钠胁迫对金盏菊生长发育的影响%Effect of Different Concentration of NaCl Stress on the Growth and Development of Calendula officinalis

    Institute of Scientific and Technical Information of China (English)

    高慧; 陈梦玲; 朱小燕

    2013-01-01

    以金盏菊为试材,研究了不同浓度NaCl(40、80、120、160、200 mmol/L)胁迫对金盏菊生长和生理特性的影响.结果表明:在不同浓度NaCl胁迫下,金盏菊的株高、茎粗、叶片数、叶片和根的鲜重与干重、叶绿素含量、蛋白质含量和可溶性糖含量均呈先上升后下降的趋势,而根长和根体积呈逐渐下降趋势.表明金盏菊耐盐阈值80 mmol/L.

  13. 城市生活污水浇灌对金盏菊生长的影响%Effects of Municipal Domestic Sewage Effluents on the Growth of Calendula officinalis

    Institute of Scientific and Technical Information of China (English)

    刘俊伟; 胡宏友; 许甘治; 陈登雄; 卢昌义

    2005-01-01

    用三种稀释倍数的城市生活污水[污水∶清水(v/v)为1﹕0、1﹕0.5、1﹕1]处理金盏菊种子及盆花,结果表明,污灌处理不仅显著地降低种子的萌发率,还增加萌发后幼苗病害的发生率;未经稀释的原生污水显著抑制金盏菊幼苗的生长,而稀释后抑制作用降低,当稀释至适当浓度时,则对幼苗生长起促进作用;对于金盏菊成年植株,污灌处理(不论稀释与否)显著地增加其茎、叶和根的鲜重,分别较对照增加161.63 %~215.12 %、86.77 %~109.23 %和23.89 %~34.13 %.综合分析表明,原生污水经过适当稀释后用于草花污灌,可以使污水中的营养盐得到回用,提高草花的观赏性.

  14. Extraction and Stability of Pigment from Calendula officinalis L.Flowers%金盏菊花中色素的提取工艺及稳定性研究

    Institute of Scientific and Technical Information of China (English)

    李泽鸿; 张晓刚; 张璐; 韩娟; 张帆; 刘洪章

    2011-01-01

    目的:确定金盏菊花色素的最佳提取工艺,并对其稳定性进行研究.方法:以浸提法对金盏菊花中的色素进行提取,通过对提取剂、料液比、提取温度、提取时间4因素进行,L9(3~4)正交试验得到最佳提取条件;同时考察光照、热和食品添加剂等对色素稳定性的影响.结果:金盏菊花色素最佳提取工艺为提取剂90%乙醇、料液比1:8(g/mL)、在70℃水浴中浸提40min;该色素在酸性条件下稳定性较好,对光和热稳定性好;pH值对色素的稳定性影响较大,在酸性条件(pH5)下该色素较稳定;常用食品添加剂如葡萄糖、蔗糖对色素的色泽无不良影响,金属离子Na+、Mg2+、Ca2+、Cu2+对金盏菊花色素无不良影响,而Fe3+则对色素有明显影响.结论:获得金盏菊花中色素提取的最佳工艺;色素对光、热、常用食品添加剂的稳定性良好,为其在食品和药品中的应用提供了广阔的前景.

  15. Effects of Drought Stress on Membrane Lipid Peroxidation and Protective Enzymes Activity in Calendula officinalis%干旱胁迫对金盏菊膜脂过氧化及保护酶活性的影响

    Institute of Scientific and Technical Information of China (English)

    谷文众; 刘杨; 谷振军

    2009-01-01

    为给金盏菊栽培技术的改进及新种选育提供理论依据,在人工模拟持续干旱条件下,研究了膜脂过氧化及保护酶活性等生理指标的变化.研究结果表明,金盏菊叶片细胞1 d内质膜透性增大,渗透调节物质和膜脂过氧化产物也相应增加,3~5 d内增大趋势不明显,其实质是POD和CAT活性先上升后下降,特别是干旱维持7 d时CAT活性低于对照水平.

  16. Growth of Jasminum sambac, Calendula officinalis, Matthiola incana, and Senecio hybridus Influenced by High-Voltage Electrostatic Field%高压静电场对茉莉等四种花卉的调控作用

    Institute of Scientific and Technical Information of China (English)

    蒋耀庭; 潘丽娜

    2003-01-01

    采用本部研制的直流高压脉冲器定时对茉莉花、金盏菊、紫罗兰和瓜叶菊进行处理.结果表明.花的开花时间均得到延长。除茉莉花的开花量有所减少外.其余三种花的开花量均有所增加。

  17. 灯盏细辛注射液治疗脑梗死30例疗效验证观测%Effects Of Calendula Officinalis L Injectio on Cerebral Infarction: A Report of 30 cases

    Institute of Scientific and Technical Information of China (English)

    何群; 陈国强; 毕清

    2005-01-01

    目的验证观测灯盏细辛治疗脑梗死的疗效.方法 60例脑梗死患者随机分为A、B两组.A组用灯盏细辛注射液20ml,1次/d,共15d.B组:用丹参注射液20ml,1次/d,共15d.结果 A组有效28例,总有效率 93.33%,B组有效22例,总有效率73.33%.两组经卡方检验,χ2=23.145,χ2(0.01)=6.63,P<0.01,两组间效率有非常显著差异.结论灯盏细辛是治疗脑梗死有效的制剂.

  18. Effect of intercropping carrot (Daucus carota L.) with french marigold (Tagetes patula nana L.) and pot marigold (Calendula officinalis L.) on the occurrence of some pests and quality of carrot yield

    OpenAIRE

    Beata Jankowska; Elżbieta Jędrszczyk; Małgorzata Poniedziałek

    2012-01-01

    Intercropping combines different aspects of the interaction between organisms in ecosystems and may be classified as a pro-ecological method of plant cultivation limiting the harmful human interference in the environment, especially the use of chemicals. It also allows high yield, good quality, and economic productivity to be achieved. The aim of the present study, conducted in the years 2003–2004 in Mydlniki near Kraków, was to determine the effect of intercropping carrot Nardin F1 with Fren...

  19. LIN总线拓扑结构分析%Analysis of application and topology in LIN bus

    Institute of Scientific and Technical Information of China (English)

    肖木; 王丽芳; 唐晓泉

    2007-01-01

    LIN(Local Interonnect Network)总线是一种新型低成本汽车车身网络低端通讯总线.通过分析LIN总线的总线负载率,得出单个主节点和多个从节点组成的LIN网络传输报文帧的上限值,并得到实验验证,为如何使用LIN总线以及LIN总线的拓扑结构布局提供了依据.

  20. The histopathological effects of salvia officinalis on the kidney and liver of rats

    Directory of Open Access Journals (Sweden)

    D.A. Adekomi

    2013-03-01

    Full Text Available The aim of this investigation was to evaluate some of the effects of aqueous leaf extract of Salvia officinalis on the kidney and liver of male Sprague Dawley rats. Ten Sprague-Dawley rats (7-11 weeks old were randomly assigned into two groups; A and B. Aqueous extract of S. officinalis leaves (300 mg/kg body weight was administered orally to the rats in group B while the rats in group A received equal volume of normal saline for 14d. At termination of treatment, the histopathology of the kidney and liver were assessed. The kidney and the liver in the extract treated rat displayed organized and preserved histological profile. Our findings suggest that S. officinalis has no deleterious effects on the kidney and liver of the rats.  

  1. The effect of hydroalcohlic extract of Valeriana officinalis on the astrocytes of hippocampus in rats

    Directory of Open Access Journals (Sweden)

    A Roozbehi

    2015-07-01

    Results: The mean numbers of astrocytes of the CA1 and CA2 in the group that received 600 mg/kg of Valeriana officinalis extracts were 16.79±6.48 and 9.11±3.91 respectively, which significantly increased compare to the control group. The mean of large diameter of astrocytes in the CA1 region of the animals with 300, 400 and 600 mg/kg of Valeriana officinalis were 10.41±2.87, 7.85±2.36 and 5.5±2.06 respectively, that decreased significantly compare to the control group 13.1±4.01. Conclusion: The Valeriana officinalis extract with antioxidant property has potential to proliferate the astrocytes cells in the hippocampus.

  2. Antiosteoporotic Activity of Anthraquinones from Morinda officinalis on Osteoblasts and Osteoclasts

    Directory of Open Access Journals (Sweden)

    Cheng-Jian Zheng

    2009-01-01

    Full Text Available Bioactivity-guided fractionation led to the successful isolation of antiosteoporotic components, i.e. physicion (1, rubiadin-1-methyl ether (2, 2-hydroxy-1-methoxy- anthraquinone (3, 1,2-dihydroxy-3-methylanthraquinone (4, 1,3,8-trihydroxy-2-methoxy- anthraquinone (5, 2-hydroxymethyl-3-hydroxyanthraquinone (6, 2-methoxyanthraquinone (7 and scopoletin (8 from an ethanolic extract of the roots of Morinda officinalis. Compounds 4-8 are isolated for the first time from M. officinalis. Among them, compounds 2 and 3 promoted osteoblast proliferation, while compounds 4, 5 increased osteoblast ALP activity. All of the isolated compounds inhibited osteoclast TRAP activity and bone resorption, and the inhibitory effects on osteoclastic bone resorption of compounds 1 and 5 were stronger than that of other compounds. Taken together, antiosteoporotic activity of M. officinalis and its anthraquinones suggest therapeutic potential against osteoporosis.

  3. Antiosteoporotic activity of anthraquinones from Morinda officinalis on osteoblasts and osteoclasts.

    Science.gov (United States)

    Wu, Yan-Bin; Zheng, Cheng-Jian; Qin, Lu-Ping; Sun, Lian-Na; Han, Ting; Jiao, Lei; Zhang, Qiao-Yan; Wu, Jin-Zhong

    2009-01-01

    Bioactivity-guided fractionation led to the successful isolation of antiosteoporotic components, i.e. physicion (1), rubiadin-1-methyl ether (2), 2-hydroxy-1-methoxy- anthraquinone (3), 1,2-dihydroxy-3-methylanthraquinone (4), 1,3,8-trihydroxy-2-methoxy- anthraquinone (5), 2-hydroxymethyl-3-hydroxyanthraquinone (6), 2-methoxyanthraquinone (7) and scopoletin (8) from an ethanolic extract of the roots of Morinda officinalis. Compounds 4-8 are isolated for the first time from M. officinalis. Among them, compounds 2 and 3 promoted osteoblast proliferation, while compounds 4, 5 increased osteoblast ALP activity. All of the isolated compounds inhibited osteoclast TRAP activity and bone resorption, and the inhibitory effects on osteoclastic bone resorption of compounds 1 and 5 were stronger than that of other compounds. Taken together, antiosteoporotic activity of M. officinalis and its anthraquinones suggest therapeutic potential against osteoporosis. PMID:19169204

  4. A new extraction method of bioflavanoids from poisonous plant (Gratiola Officinalis L.

    Directory of Open Access Journals (Sweden)

    Natalya V. Polukonova

    2014-09-01

    Full Text Available The way of vegetable raw materials extraction which allows to receive nontoxical composition of biological active agents from poisonous plants such as Gratiola officinalis L. was described. The alkaloids exit changes with the increase of ethyl alcohol percentage (from 15% to 96%. The extract was obtained using 96% ethanol and did not give positive high quality reaction to the content of alkaloids. The chemical composition with new nontoxical biological active composition of Gratiola officinalis L. extract was investigated. The extract contains a previously unknown plant – bioflavonoid quercetin. The average value of quercetin in this extract using the calibration curve of the standard sample quercetin (98% Sigma is 0.66%. In the dry rest of extractive substances (Gratiola officinalis L. the quantity of quercetin was 350 mkg (obtained from 10 g of a dry grass as was established by the method of a liquid chromatography.

  5. Acute atropine intoxication with psychiatric symptoms by herbal infusion of Pulmonaria officinalis (Lungwort

    Directory of Open Access Journals (Sweden)

    Enrique Baca-García

    2007-06-01

    Full Text Available Background and objectives: Lungwort infusion is a preparation extracted from Pulmonaria officinalis which is occasionally used as a folk remedy for the common cold. The current report aims to describe acute atropine intoxications with delirium caused by Lungwort infusion in several members of the same family. Methods: Description of three case reports. Search of literature through Medline. Results: Three generations of a same family presented acute and moderately severe atropine intoxications after drinking an infusion prepared with Pulmonaria officinalis. Conclusions: Despite the lack of scientific evidence for its clinical use, medicinal plants continue being widely used. In spite of severe adverse effects reported, the general thought is that herbal remedies are harmless. To our knowledge, this is the first report of acute atropine intoxications with psychiatric symptoms secondary to Pulmonaria officinalis in several members of a family. We suspect that the lungwort infusion may have been contaminated with some other substance with atropinic properties.

  6. A simple and efficient DNA isolation method for Salvia officinalis.

    Science.gov (United States)

    Aleksić, Jelena M; Stojanović, Danilo; Banović, Bojana; Jančić, Radiša

    2012-12-01

    We report an efficient, simple, and cost-effective protocol for the isolation of genomic DNA from an aromatic medicinal plant, common sage (Salvia officinalis L.). Our modification of the standard CTAB protocol includes two polyphenol adsorbents (PVP 10 and activated charcoal), high NaCl concentrations (4 M) for removing polysaccharides, and repeated Sevag treatment to remove proteins and other carbohydrate contaminants. The mean DNA yield obtained with our Protocol 2 was 330.6 μg DNA g(-1) of dry leaf tissue, and the absorbance ratios 260/280 and 260/230 nm averaged 1.909 and 1.894, respectively, revealing lack of contamination. PCR amplifications of one nuclear (26S rDNA) and one chloroplast (rps16-trnK) locus indicated that our DNA isolation protocol may be used in common sage and other aromatic and medicinal plants containing essential oil for molecular biologic and biotechnological studies and for population genetics, phylogeographic, and conservation surveys in which nuclear or chloroplast genomes would be studied in large numbers of individuals.

  7. Salvia Officinalis and Cisplatin Effects on Pentylenetetrazole Induced Seizure Threshold

    Directory of Open Access Journals (Sweden)

    Mir Hadi Khayate-Nouri

    2013-11-01

    Full Text Available Background: Studies have shown that cisplatin have neuropathic effects and Salvia officinalis (SO could have therapeutic effects on nervous system. The aim of this study was to investigate the effects of SO hydroalcoholic extract and cisplatin on pentylenetetrazole (PTZ induced seizure in mice. Materials and methods: This is an experimental interventional study. For this purpose first group received normal saline, second group received SO extract, third group received cisplatin, in the fourth group received SO extract plus cisplatin and the subsequent seizure threshold was determined for each group. Results: The results showed that SO extract significantly (p<0.05 increased and in cisplatin group significantly (p<0.05 decreased seizure threshold. Simultaneous uses of cisplatin and SO extract caused to significantly increased seizure threshold (p<0.05 compared with cisplatin group. Conclusion: Considering different types of ingredients in SO extract which have beneficial effects on nervous system, it might be used to reduce cisplatin induced neuropathic effects. It seems that SO extract could be useful in cisplatin-induced seizure but further investigations are needed.

  8. Anxiolytic Effect of Borago officinalis (Boraginaceae Extract in Male Rats

    Directory of Open Access Journals (Sweden)

    Komaki

    2015-02-01

    Full Text Available Background Medicinal plants with natural antioxidants have been shown to be beneficial in a variety of complications such as anxiety. The elevated plus-maze (EPM is one of the most widely used models to assess anxiety in small rodents. Objectives This study was designed to characterize the anxiolytic-like activity of Borago officinalis (Linnaeus, family Boraginaceae or Borage flowers extract, using an EPM test. Materials and Methods Male Wistar rats weighing 220-250 grams were used in the present study. Thirty minutes after an intraperitoneal (IP injection of the Borage extract (50, 100, 200 mg/kg or saline, each animal was placed in the EPM. Animal behaviors in the experimental sessions were recorded by a video camera located above the maze, interfaced with a monitor and a computer in an adjacent room. The time spent in the open arms, the percentage of entries into the open arms of the EPM and the numbers of entries into the closed arms were recorded for five minutes. Results Statistical analysis indicated that acute IP injection of Borage extract before an EPM trial significantly increased the time spent in open arms and percentage of open arms entries. Whereas, the extract had no effect on the number of closed arm entries. Conclusions Our results demonstrated that injection of Borage extract might have an anxiolytic profile in rats. However, the exact mechanism (s related to the active compound (s in Borage extract should be elucidated in future studies.

  9. Rosmarinus officinalis extract suppresses Propionibacterium acnes-induced inflammatory responses.

    Science.gov (United States)

    Tsai, Tsung-Hsien; Chuang, Lu-Te; Lien, Tsung-Jung; Liing, Yau-Rong; Chen, Wei-Yu; Tsai, Po-Jung

    2013-04-01

    Propionibacterium acnes is a key pathogen involved in the progression of acne inflammation. The development of a new agent possessing antimicrobial and anti-inflammatory activity against P. acnes is therefore of interest. In this study, we investigated the inhibitory effect of rosemary (Rosmarinus officinalis) extract on P. acnes-induced inflammation in vitro and in vivo. The results showed that ethanolic rosemary extract (ERE) significantly suppressed the secretion and mRNA expression of proinflammatory cytokines, including interleukin (IL)-8, IL-1β, and tumor necrosis factor-α in P. acnes-stimulated monocytic THP-1 cells. In an in vivo mouse model, concomitant intradermal injection of ERE attenuated the P. acnes-induced ear swelling and granulomatous inflammation. Since ERE suppressed the P. acnes-induced nuclear factor kappa-B (NF-κB) activation and mRNA expression of Toll-like receptor (TLR) 2, the suppressive effect of ERE might be due, at least partially, to diminished NF-κB activation and TLR2-mediated signaling pathways. Furthermore, three major constituents of ERE, carnosol, carnosic acid, and rosmarinic acid, exerted different immumodulatory activities in vitro. In brief, rosmarinic acid significantly suppressed IL-8 production, while the other two compounds inhibited IL-1β production. Further study is needed to explore the role of bioactive compounds of rosemary in mitigation of P. acnes-induced inflammation. PMID:23514231

  10. Rosmarinus Officinalis Leaves as a Natural Source of Bioactive Compounds

    Directory of Open Access Journals (Sweden)

    Isabel Borrás-Linares

    2014-11-01

    Full Text Available In an extensive search for bioactive compounds from plant sources, the composition of different extracts of rosemary leaves collected from different geographical zones of Serbia was studied. The qualitative and quantitative characterization of 20 rosemary (Rosmarinus officinalis samples, obtained by microwave-assisted extraction (MAE, was determined by high performance liquid chromatography coupled to electrospray quadrupole-time of flight mass spectrometry (HPLC–ESI-QTOF-MS. The high mass accuracy and true isotopic pattern in both MS and MS/MS spectra provided by the QTOF-MS analyzer enabled the characterization of a wide range of phenolic compounds in the extracts, including flavonoids, phenolic diterpenes and abietan-type triterpenoids, among others. According to the data compiled, rosemary samples from Sokobanja presented the highest levels in flavonoids and other compounds such as carnosol, rosmaridiphenol, rosmadial, rosmarinic acid, and carnosic acid. On the other hand, higher contents in triterpenes were found in the extracts of rosemary from Gložan (Vojvodina.

  11. [Salvia officinalis l. I. Botanic characteristics, composition, use and cultivation].

    Science.gov (United States)

    Daniela, T

    1993-06-01

    Salvia officinalis L. is an essential oil containing plant, which does not wildly grow in the territories of the Czech and Slovak Republics but it can be successfully cultivated. It is a perennial half-shrub, from which non-flowering herbaceous sprouts or leaves are collected for pharmaceutical purposes. After drying at a temperature not exceeding 35 degrees C they are the plant drugs Herba salviae or Folium salviae. In PhBs, Herba salviae is official. The drug contains mainly ethereal oil (1-2%), diterpenes, triterpenes and tannin. The pharmacopoeial criterion of quality is the content of essential oil, which is produced in an increased amount in the plant in warm summer months. Herba salviae and the extracts prepared from it are used as an antiseptic agent, an antiphlogistic agent, in the inflammations of the oral cavity and gingivitis and also as a stomachic and an antihydrotic agent. Its utilization in cosmetics and food industry is also of importance. PMID:8402963

  12. Genome-wide identification and validation of simple sequence repeats (SSRs) from Asparagus officinalis.

    Science.gov (United States)

    Li, Shufen; Zhang, Guojun; Li, Xu; Wang, Lianjun; Yuan, Jinhong; Deng, Chuanliang; Gao, Wujun

    2016-06-01

    Garden asparagus (Asparagus officinalis), an important vegetable cultivated worldwide, can also serve as a model dioecious plant species in the study of sex determination and sex chromosome evolution. However, limited DNA marker resources have been developed and used for this species. To expand these resources, we examined the DNA sequences for simple sequence repeats (SSRs) in 163,406 scaffolds representing approximately 400 Mbp of the A. officinalis genome. A total of 87,576 SSRs were identified in 59,565 scaffolds. The most abundant SSR repeats were trinucleotide and tetranucleotide, accounting for 29.2 and 29.1% of the total SSRs, respectively, followed by di-, penta-, hexa-, hepta-, and octanucleotides. The AG motif was most common among dinucleotides and was also the most frequent motif in the entire A. officinalis genome, representing 14.7% of all SSRs. A total of 41,917 SSR primers pairs were designed to amplify SSRs. Twenty-two genomic SSR markers were tested in 39 asparagus accessions belonging to ten cultivars and one accession of Asparagus setaceus for determination of genetic diversity. The intra-species polymorphism information content (PIC) values of the 22 genomic SSR markers were intermediate, with an average of 0.41. The genetic diversity between the ten A. officinalis cultivars was low, and the UPGMA dendrogram was largely unrelated to cultivars. It is here suggested that the sex of individuals is an important factor influencing the clustering results. The information reported here provides new information about the organization of the microsatellites in A. officinalis genome and lays a foundation for further genetic studies and breeding applications of A. officinalis and related species. PMID:26987412

  13. COMBINED ENDOCRINE EFFECTS OF IN UTERO EXPOSURE TO THE ANTIANDROGENS BUTYLBENZYL PHTHALATE (BBP) AND LINURON (LIN) ON FETAL TESTOSTERONE (T) SYNTHESIS AND REPRODUCTIVE TRACT DEVELOPMENT

    Science.gov (United States)

    COMBINED ENDOCRINE EFFECTS OF IN UTERO EXPOSURE TO THE ANTIANDROGENS BUTYLBENZYL PHTHALATE (BBP) AND LINURON (Lin) ON FETAL TESTOSTERONE (T) SYNTHESIS AND REPRODUCTIVE TRACT DEVELOPMENTParks LG , Hotchkiss AK, Ostby J, Lambright C and Gray LE, Jr.Lin and BBP are toxic...

  14. Influence of light intensity on growth and physiological characteristics of common sage (Salvia officinalis L.

    Directory of Open Access Journals (Sweden)

    George Zervoudakis

    2012-02-01

    Full Text Available The aim of this work was to investigate the effects of four different light intensities on the growth characteristics, physiological parameters and leaf photosynthetic pigments of Salvia officinalis L. The plant's dry mass, number of the leaves and physiological parameters indicated a strong positive correlation with the light intensity. On the other hand, the plant's height and leaf photosynthetic pigments were increased at low light treated plants. These results suggest that the aromatic herb Salvia officinalis L. is adaptable to different light environments.

  15. The Antinociceptive Effects of Hydroalcoholic Extract of Borago Officinalis Flower in Male Rats Using Formalin Test

    OpenAIRE

    Shahraki, Mohammad Reza; Ahmadimoghadm, Mahdieh; Shahraki, Ahmad Reza

    2015-01-01

    Introduction: Borago officinalis flower (borage) is a known sedative in herbal medicine; the aim of the present study was to evaluate the antinociceptive effect of borage hydroalcoholic extract in formalin test male rats. Methods: Fifty-six adult male albino Wistar rats were randomly divided into seven groups: Control groups of A (intact), B (saline), and C (Positive control) plus test groups of D, E, F, and G (n=8). The groups D, E, and F received 6.25, 12.5, and 25 mg/kg, Borago officinalis...

  16. Cornus officinalis Methanol Extract Upregulates Melanogenesis in Melan-a Cells.

    Science.gov (United States)

    An, Yun Ah; Hwang, Ji Yeon; Lee, Jae Soon; Kim, Young Chul

    2015-06-01

    Cornus officinalis is widely distributed in Korea, and its fruit has been used to make as herbal drug for traditional medicine in Korea, Japan, and China because of its tonic, analgesic, and diuretic properties. However, the effects of C. officinalis methanol extract (COME) on melanogenesis remain poorly understood. We evaluated the melanogenic capability of COME in melan-a cells, which are immortalized mouse melanocytes. COME increased melanin synthesis in a dose-dependent manner. Treatment with 12.5 μg/mL of COME significantly increased melanin content by 36.1% (p hair graying. PMID:26191383

  17. A preliminary analysis of sleep-like states in the cuttlefish Sepia officinalis.

    Directory of Open Access Journals (Sweden)

    Marcos G Frank

    Full Text Available Sleep has been observed in several invertebrate species, but its presence in marine invertebrates is relatively unexplored. Rapid-eye-movement (REM sleep has only been observed in vertebrates. We investigated whether the cuttlefish Sepia officinalis displays sleep-like states. We find that cuttlefish exhibit frequent quiescent periods that are homeostatically regulated, satisfying two criteria for sleep. In addition, cuttlefish transiently display a quiescent state with rapid eye movements, changes in body coloration and twitching of the arms, that is possibly analogous to REM sleep. Our findings thus suggest that at least two different sleep-like states may exist in Sepia officinalis.

  18. Ribosome-inactivating proteins from the seeds of Saponaria officinalis L. (soapwort), of Agrostemma githago L. (corn cockle) and of Asparagus officinalis L. (asparagus), and from the latex of Hura crepitans L. (sandbox tree).

    Science.gov (United States)

    Stirpe, F; Gasperi-Campani, A; Barbieri, L; Falasca, A; Abbondanza, A; Stevens, W A

    1983-12-15

    Ribosome-inactivating proteins, similar to those already known [Barbieri & Stirpe (1982) Cancer Surveys 1, 489-520] were purified from the seeds of Saponaria officinalis (two proteins), of Agrostemma githago (three proteins), and of Asparagus officinalis (three proteins), and from the latex of Hura crepitans (one protein). The yield ranged from 8 to 400 mg/100 g of starting material. All proteins have an Mr of approx. 30000 and an alkaline isoelectric point. Their sugar content varies from 0 (proteins from S. officinalis) to 40% (protein from H. crepitans). The ribosome-inactivating proteins inhibit protein synthesis by rabbit reticulocyte lysate, the ID50 (concentration giving 50% inhibition) ranging from 1 ng/ml (a protein from S. officinalis) to 18 ng/ml (a protein from A. githago). Those which were tested (the proteins from S. officinalis and from A. githago) also inhibit polymerization of phenylalanine by isolated ribosomes, acting in an apparently catalytic manner. The protein from H. crepitans inhibited protein synthesis by HeLa cells, with an ID50 of 4 micrograms/ml, whereas the proteins from S. officinalis and from A. githago had an ID50 of more than 50-100 micrograms/ml. The ribosome-inactivating proteins from S. officinalis and from A. githago reduced the number of local lesions by tobacco-mosaic virus in the leaves of Nicotiana glutinosa.

  19. Physical location of rice Gm-6,Pi-5(t) genes in O. officinalis with BAC-FISH

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    A fluorescence in situ hybridization (FISH) procedure was adoptedto physically map two rice BAC clones 24E21 and 4F22 linked to Gm-6 and Pi-5(t) in O. officinalis. FISH results showed that the two BAC clones were located at 4L. The percentage distance from the centromere to the hybridization sites was 72±2.62 for 24E21 and 54±5.43 for 4F22, the detection rates were 52.70% and 61.2%. The results obtained from the BAC and plasmid clones, RG214 and RZ565 of cultivated rice and O. officinalis were the same. This suggested that the markers, RG214 and RZ565 of cultivated rice and O. officinalis were in the same BAC clones. The homologous sequences of Gm-6 and Pi-5(t) in O. officinalis were positions that signals existed on the 4L. Many signals were observed when no Cot-1 DNA blocked. This also showed that repetitive sequences were some homolgous between cultivated rice and O. officinalis. The identification of chromosome 4 of O. officinalis is based on Jena et al. (1994). In our study, we discussed the possibility of physical map in O. officinalis with rice BAC clones.

  20. An Effective Implementation of K-opt Moves for the Lin-Kernighan TSP Heuristic

    DEFF Research Database (Denmark)

    Helsgaun, Keld

    Local search with k-change neighborhoods, k-opt, is the most widely used heuristic method for the traveling salesman problem (TSP). This report presents an effective implementation of k-opt for the Lin- Kernighan TSP heuristic. The effectiveness of the implementation is demonstrated with extensive...

  1. Effect of Ethanolic Extract of Emblica officinalis on Histopathology of Kidney and on Biochemical Parameters in Hyperlipidemic Albino Rats

    Directory of Open Access Journals (Sweden)

    Bheemshetty S. Patil

    2015-01-01

    Full Text Available Background: It has been reported that hyperlipidemia plays a central role in the development of atherosclerosis and oxidative stress. Embilica officinalis also known as Amla or Indian Gooseberry acts as antihyperlipidemic and antioxidant. Its active ingredients contains tannins, gallic acid and flavonoids. Aim & Objectives: It was aimed to evaluate the effect of ethanolic extract of Emblica officinalis on histopathology of kidney and on biochemical parameters in hyperlipidemic albino Wistar rats. Material and Methods: Extraction of dried fruits of Emblica officinalis was done by Soxhlet apparatus 0 using 99% ethanol at 60 C for 24 hours and also phytochemical analysis was done. Group I served as normal control. Group II was fed with isocaloric diet. Group III was fed with hyperlipidemic diet. Group IV was fed with isocaloric diet for 21 days + Embilica officinalis for 21 days. Group V was fed with hyperlipidemic diet for 21 days+ Embilica officinalis for 21 days. The dose of ethanolic extract of Emblica officinalis was taken as 100mg/kg body weight daily. Results: Percent body weight gain, kidney weight and nephro-somatic index significantly improved in hyperlipidemic rats treated with Emblica officinalis. There was a significant improvement in serum electrolyte and kidney markers. It was found that there were focal glomerular lesions with thickening of glomerulus in the kidneys of rats on hyperlipidemic diet and normal renal histology of rats on hyperlipidemic diet treated with Emblica officinalis. Conclusion: It can be concluded that Emblica officinalis may be a good, natural therapeutic agent against hyperlipidemic diet induced oxidative damage and nephrotoxicity.

  2. Minerals detection for hyperspectral images using adapted linear unmixing: LinMin

    CERN Document Server

    Frederic, Schmidt; Stephane, Le Mouelic

    2014-01-01

    Minerals detection over large volume of spectra is the challenge addressed by current hyperspectral imaging spectrometer in Planetary Science. Instruments such OMEGA (Mars Express), CRISM (Mars Reconnaissance Orbiter), M^{3} (Chandrayaan-1), VIRTIS (Rosetta) and many more, have been producing very large datasets since one decade. We propose here a fast supervised detection algorithm called LinMin, in the framework of linear unmixing, with innovative arrangement in order to treat non-linear cases due to radiative transfer in both atmosphere and surface. We use reference laboratory and synthetic spectral library. Additional spectra are used in order to mimic the effect of Martian aerosols, grain size, and observation geometry discrepancies between reference and observed spectra. The proposed algorithm estimates the uncertainty on mixing coefficient from the uncertainty of observed spectra. Both numerical and observational tests validate the approach. Fast parallel implementation of the best algorithm (IPLS) on ...

  3. QUANTUM INSPIRED PARTICLE SWARM COMBINED WITH LIN-KERNIGHAN-HELSGAUN METHOD TO THE TRAVELING SALESMAN PROBLEM

    Directory of Open Access Journals (Sweden)

    Bruno Avila Leal de Meirelles Herrera

    2015-12-01

    Full Text Available ABSTRACT The Traveling Salesman Problem (TSP is one of the most well-known and studied problems of Operations Research field, more specifically, in the Combinatorial Optimization field. As the TSP is a NP (Non-Deterministic Polynomial time-hard problem, there are several heuristic methods which have been proposed for the past decades in the attempt to solve it the best possible way. The aim of this work is to introduce and to evaluate the performance of some approaches for achieving optimal solution considering some symmetrical and asymmetrical TSP instances, which were taken from the Traveling Salesman Problem Library (TSPLIB. The analyzed approaches were divided into three methods: (i Lin-Kernighan-Helsgaun (LKH algorithm; (ii LKH with initial tour based on uniform distribution; and (iii an hybrid proposal combining Particle Swarm Optimization (PSO with quantum inspired behavior and LKH for local search procedure. The tested algorithms presented promising results in terms of computational cost and solution quality.

  4. Traitement statistique des distorsions non-linéaires pour la restauration des enregistrements sonores.

    OpenAIRE

    Picard, Guillaume

    2006-01-01

    L'objet de la thèse est l'étude, la modélisation et le traitement des distorsions non linéaires sonores, pour lesquelles les techniques actuelles s'avèrent impuissantes. L'approche retenue consiste à représenter, globalement, à la fois le signal audio à restaurer et le processus de distorsion, dans le cadre d'un modèle statistique. Cette approche présente un bon compromis entre une souhaitable généricité -possibilité de traiter à l'aide d'une méthode globale plusieurs types de distorsions- et...

  5. Measurement of the 1H(6He,6Li)n reaction in inverse kinematics

    International Nuclear Information System (INIS)

    The 1H(6He,6Li)n reaction was studied at 0 degree with the NSCL A1200 fragment separator in the energy loss mode. A 6He secondary beam at E/A=93 MeV was used to measure the Gamow-Teller and Fermi strengths between the ground state of 6He and the ground and excited states of 6Li, in inverse kinematics. At 0 degree the ground-state cross section is measured to be dσGS/(dΩ)=43±16 mb/sr, which is dominated by systematic error in the secondary beam flux. The ratio of Gamow-Teller to Fermi strength is not sensitive to this error and is found to be (87±6)% of that expected from (p,n) systematics and β decay. Angular distributions have been measured between 0 degree and 10 degree in the center of mass. copyright 1996 The American Physical Society

  6. Reduction of EMC Emissions in Mixed Signal Integrated Circuits with Embedded LIN Driver

    Directory of Open Access Journals (Sweden)

    P. Hartl

    2016-06-01

    Full Text Available This paper describes several methods for reduction of electromagnetic emissions (EME of mixed signal integrated circuits (IC. The focus is on the impact that a LIN bus communication block has on a complex IC which contains analog blocks, noisy digital block, micro-core (µC and several types of memories. It is used in an automotive environment, where EMC emission reduction is one of the key success factors. Several proposed methods for EME reduction are described and implemented on three test chips. These methods include current consumption reduction, internal on-chip decoupling, ground separation and different linear voltage regulator topologies. Measurement results of several fabricated test chips are shown and discussed.

  7. Méthodes non linéaires pour des problèmes statistiques inverses

    OpenAIRE

    Barbillon, Pierre; Celeux, Gilles; Grimaud, Agnès; Lefebvre, Yannick; De Rocquigny, Etienne

    2010-01-01

    Dans le cadre du traitement des incertitudes étudié ici, la variabilité intrinsèque des entrées d'un modèle physique est modélisée par une loi de probabilité multivariée. L'objectif est d'identifier cette loi de probabilité à partir d'observations des sorties du modèle. Afin de se limiter à un nombre d'appels raisonnable au code de calcul (souvent coûteux) du modèle physique dans l'algorithme d'inversion, une méthodologie d'approximation non linéaire faisant intervenir le krigeage et un algor...

  8. José Lins do Rego: um guia brasileiro de Israel

    Directory of Open Access Journals (Sweden)

    Glauber Pereira Quintão

    2010-03-01

    Full Text Available Este artigo procura analisar, de forma crítica, o livro de José Lins do Rego, Roteiro de Israel, 1955. Busca-­se destacar duas facetas suplementares: a de um olhar poético e a de uma preocupação ético-política lançados pelo escritor sobre a construção do novo Estado de Israel. Observa-­se também uma relação entre os signos da modernidade e das histórias arcaica judaicas. Para isso, usa-­se o conceito de palimpsesto, de Gerard Genette, e de flâneur, de Walter Benjamim.

  9. Baker- Lin-Huang type Bivariate distributions based on order statistics

    CERN Document Server

    Bairamov, I

    2011-01-01

    Baker (2008) introduced a new class of bivariate distributions based on distributions of order statistics from two independent samples of size n. Lin-Huang (2010) discovered an important property of Baker's distribution and showed that the Pearson's correlation coefficient for this distribution converges to maximum attainable value, i.e. the correlation coefficient of the Frech\\'et upper bound, as n increases to infinity. Bairamov and Bayramoglu (2011) investigated a new class of bivariate distributions constructed by using Baker's model and distributions of order statistics from dependent random variables, allowing high correlation than that of Baker's distribution. In this paper a new class of Baker's type bivariate distributions with high correlation are constructed on the base of distributions of order statistics by using an arbitrary continuous copula instead of the product copula. Keywords: Bivariate distribution function, FGM distributions, copula, positive quadrant dependent, negative quadrant depende...

  10. EhLIN tokamak for investigation and development of electrical equipment

    International Nuclear Information System (INIS)

    Description of some systems of the EhLIN tokamak, designed for carrying out investigations aimed at development of cryoresistive toroidal solenoids with the magnetic field in the axis 8-10 T and their power supply is given. Modular toroidal solenoids made in the form of a wedge-shaped copper band coil are developed. Rein forced banding of the coil is performed by a glassroving with mechanical strength 480 kg/mm2. A high-voltage diode unit representing a cylinder with the diameter of 23 cm, 70 cm height and the weight of 40 kg is developed for solenoid power supply. The unit operateds at reverse voltage 10 kV and permits to conduct current upto 40 kA at pulse duration 10 ms as well as direct current 2500 A during natural air cooling. The unit has inductance 10-7 H

  11. XSHOOTER spectroscopy of the enigmatic planetary nebula Lin49 in the Small Magellanic Cloud

    Science.gov (United States)

    Otsuka, Masaaki; Kemper, F.; Leal-Ferreira, M. L.; Aleman, I.; Bernard-Salas, J.; Cami, J.; Ochsendorf, B. B.; Peeters, E.; Scicluna, P.

    2016-10-01

    We performed a detailed spectroscopic analysis of the fullerene C60-containing planetary nebula (PN) Lin49 in the Small Magellanic Cloud (SMC) using XSHOOTER at the European Southern Observatory Very Large Telescope and the Spitzer/Infrared Spectrograph instruments. We derived nebular abundances for nine elements. We used TLUSTY to derive photospheric parameters for the central star. Lin49 is C-rich and metal-deficient PN (Z ˜ 0.0006). The nebular abundances are in good agreement with asymptotic giant branch nucleosynthesis models for stars with initial mass 1.25 M⊙ and metallicity Z = 0.001. Using the TLUSTY synthetic spectrum of the central star to define the heating and ionizing source, we constructed the photoionization model with CLOUDY that matches the observed spectral energy distribution (SED) and the line fluxes in the UV to far-IR wavelength ranges simultaneously. We could not fit the ˜1-5 μm SED using a model with 0.005-0.1-μm-sized graphite grains and a constant hydrogen density shell owing to the prominent near-IR excess, while at other wavelengths the model fits the observed values reasonably well. We argue that the near-IR excess might indicate either (1) the presence of very small particles in the form of small carbon clusters, small graphite sheets, or fullerene precursors, or (2) the presence of a high-density structure surrounding the central star. We found that SMC C60 PNe show a near-IR excess component to lesser or greater degree. This suggests that these C60 PNe might maintain a structure nearby their central star.

  12. Flowering biology and nectary structure of Melissa officinalis L.

    Directory of Open Access Journals (Sweden)

    Mirosława Chwil

    2012-12-01

    Full Text Available The present study on lemon balm (Melissa officinalis L. covered flowering biology, monitoring of pollinating insects and floral nectary structure. The micromorphology of epidermal cells of the nectary was investigated using scanning electron microscopy. The nectariferous tissues were observed using light microscopy based on semi-thin sections. Lemon balm flowered from the second decade of June until September. Buds opened from early morning hours until noon. Flowers lived for 24 hours, on the average. Their primary pollinator was the honey bee. The beginning of nectar secretion was found to be at the bud swell stage. The automorphic nectary forms a disc with four protrusions at the base of the nectary. Three smaller ones and one larger than the other ones were distinguished among them. No stomata were found on the lower protuberances, whereas on the highest part anomocytic stomata were present, the number of which was 15. The stomata exhibited different development stages and they were situated above other epidermal cells. In their outline, they were ellipsoidally shaped (18 × 23 µm and they had average-sized cuticular ledges. They produced a smooth cuticle and wax granules. In cross section, the nectary tissues were composed of a singlelayered epidermis and 9 - 11 layers of the nectary parenchyma. Their thickness was 198 µm. In longitudinal section, the height of the nectary was within a range of 354 - 404 µm. The epidermal cells produced thin outer cell walls. Some of them were completely filled with strongly stained cytoplasm, whereas others showed a high degree of vacuolisation. But the nectary parenchyma cells were marked by poorly stained cytoplasm, a large nucleus and vacuolisation of varying degree.

  13. Comparison of Antioxidant and Antimicrobial Properties for Ginkgo biloba and Rosemary (Rosmarinus officinalis L. from Egypt

    Directory of Open Access Journals (Sweden)

    Hossam S. EL-BELTAGI

    2013-05-01

    Full Text Available The widespread use of medicinal plants for health purposes has increased dramatically due to their great importance to the public health. In this study levels of phenolic, flavonoid contents of Ginkgo biloba and Rosmarinus officinalis from Egypt were determined. HPLC was used to identify and quantify the phenolic compounds in selected plants. The plant extracts were evaluated for their antioxidant activities using various antioxidant methodologies, (i scavenging of free radicals using 2, 2-diphenyl-1-picrylhydrazyl, (ii metal ion chelating capacity, and (iii scavenging of superoxide anion radical. The antimicrobial activity of both plant’s extracts were evaluated against a panel of microorganisms by using agar disc diffusion method. The total phenolic content (75.30 and 98.31 mg/g dry weight in G. biloba and R. officinalis, respectively was significantly (p<0.05 different. Among the identified phenolic compounds, quercetin, kaempferol and caffeic acid were the predominant phenolic compounds in Ginkgo biloba, whereas carnosic acid, rosmarinic acid, narinigen and hispidulin were the predominant phenolic compound in Rosmarinus officinalis leaves. The antioxidant activity increased with the concentration increase. The R. officinalis was more active than G. biloba extract against Gram-negative bacteria. This study reveals that the consumption of these plants would exert several beneficial effects by virtue of their antioxidant and antimicrobial activities.

  14. Antioxidant activity and sensory assessment of a rosmarinic acid-enriched extract of Salvia officinalis

    Science.gov (United States)

    An extract of S. officinalis (garden sage) was prepared using supercritical carbon dioxide (SC-CO2) extraction, followed by a Soxhlet hot water extraction. The resulting extract was enriched in polyphenols, including rosmarinic acid (RA), which has shown promising health benefits in animals. The ext...

  15. Dalmatian Sage (Salvia officinalis L.: A Review of Biochemical Contents, Medical Properties and Genetic Diversity

    Directory of Open Access Journals (Sweden)

    Martina Grdiša

    2015-11-01

    Full Text Available Dalmatian sage (Salvia officinalis L. represents one of the most significant medicinal autochthonous species in flora of eastern Adriatic coast and islands. It is evergreen outcrossing perennial subshrub with short woody stems that branch extensively and violet flowers. Apart from being native to Mediterranean karst of west Balkan and Apenine peninsula it is cultivated in numerous countries worldwide with Mediterranean and temperate continental climate. From the earliest times it has been used in traditional medicine in healing gingiva, mouth cavity and the sore throat, against bacterial and fungal infections, for wound treatment, memory enhancement, for treating common cold, against sweating, stomach inflammation, ulcer formation, etc. Its essential oil has also been used in preservation of food and as spice as it gives both specific aroma and promotes digestion of food. The essential oil is extremely complex mixture of different active ingredients; however, the thujones and camphor are the dominant compounds and are the parameter by which S. officinalis is distinguished from other Salvia species. The great variability of essential oil composition and yield has been detected depending on various factors such as genotype, environmental conditions, phonological stage, plant parts used for the extraction of essential oil and drying procedure. Molecular genetic analysis of S. officinalis is still limited and comprises the use of RAPD markers, AFLP and SSR markers in assessing mostly the genetic variability and structure of wild S. officinalis populations.

  16. Matrix solid-phase dispersion for the liquid chromatographic determination of phenolic acids in Melissa officinalis.

    Science.gov (United States)

    Ziaková, Alica; Brandsteterová, Eva; Blahová, Eva

    2003-01-01

    Matrix solid-phase dispersion (MSPD) was used for sample preparation of plant material (Melissa officinalis, Lemon Balm) prior to liquid chromatography of rosmarinic, caffeic and protocatechuic acids, phenolic compounds present in this herb. Different MSPD sorbents and various elution agents were tested and the optimal extraction conditions determined with the aim to obtain extraction recoveries greater than 90% for all analytes. PMID:12568390

  17. Antioxidant activity and sensory evaluation of a rosmarinic acid-enriched extract of Salvia officinalis

    Science.gov (United States)

    An extract of S. officinalis (garden sage) was developed using supercritical fluid extraction, followed by hot water extraction. The resulting extract was enriched in polyphenols, including rosmarinic acid (RA), which has shown promising health benefits in animals. The extract contained RA at a conc...

  18. Larvicidal activity of essential extract of Rosmarinus officinalis against Culex quinquefasciatus.

    Science.gov (United States)

    Yu, Jing; Liu, Xiang-Yi; Yang, Bin; Wang, Jie; Zhang, Fu-Qiang; Feng, Zi-Liang; Wang, Chen-Zhu; Fan, Quan-Shui

    2013-03-01

    Constituents in rosemary (Rosmarinus officinalis) have been shown to have larvicidal activity against invertebrates. In order to explore the properties of crude extract of rosemary further, we studied the chemical composition and its activity against dichlorodiphenyltrichloroethane (DDT)-susceptible, DDT-resistant, and field strains of Culex quinquefasciatus larvae. The major components of R. officinalis were found to be eucalyptol and camphor, with relative percentages of 10.93% and 5.51%, respectively. Minor constituents included limonene, (+)-4-carene, isoborneol, 1-methyl-4-(1-methylethylidene)-cyclohexene, and pinene. The median lethal concentration (LC50) values of the essential oil of R. officinalis against DDT-susceptible, DDT-resistant, and field strains of larvae of Cx. quinquefasciatus were 30.6, 26.4, and 38.3 mg/liter, respectively. The single median lethal dose (LD50) in Kunming mice was 4752 mg/kg. Essential oils from R. officinalis may, therefore, provide an effective natural plant product for use in mosquito prevention and control. PMID:23687854

  19. Microencapsulation of Rosmarinus officinalis L. (rosemary) aqueous extract for application in functional foods

    OpenAIRE

    Ribeiro, Andreia; Caleja, Cristina; Barros, Lillian; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.; Barreiro, M.F.

    2015-01-01

    Rosmarinus officinalis L., commonly referred as rosemary, is native of the Mediterranean region being its leaf extracts normally used in traditional medicine. In particular, its phenolic extracts have been demonstrating hepatoprotective, antihyperglycemic, antiulcerogenic and antibacterial properties [1]. However, it should be highlighted that the bioactive compounds when exposed at adverse conditions (extreme pH, light, moisture, storage, food processing conditions) are generally...

  20. [Urine metabonomic study of intervention effects of Morinda officinalis how. on 'kidney-yang deficiency syndrome'].

    Science.gov (United States)

    Zou, Zhong-jie; Xie, Yuan-yuan; Gong, Meng-juan; Han, Bin; Wang, Shu-mei; Liang, Sheng-wang

    2013-11-01

    To investigate the intervention effects of Morinda officinalis How. on 'Kidney-yang deficiency syndrome' induced by hydrocortisone in rats, the metabolic profiles of rat urine were characterized using proton nuclear magnetic resonance and principal component analysis (PCA) was applied to study the trajectory of urinary metabolic phenotype of rats with 'Kidney-yang deficiency syndrome' under administration of M. officinalis at different time points. Meanwhile, the intervention effects of M. officinalis on urinary metabolic potential biomarkers associated with 'Kidney-yang deficiency syndrome' were also discussed. The experimental results showed that in accordance to the increased time of administration, an obvious tendency was observed that clustering of the treatment group moved gradually closed to that of the control group. Eight potential biomarkers including citrate, succinate, alpha-ketoglutarate, lactate, betaine, sarcosine, alanine and taurine were definitely up- or down-regulated. In conclusion, the effectiveness of M. oficinalis on 'Kidney-yang deficiency syndrome' is proved using the established metabonomic method and the regulated metabolic pathways involve energy metabolism, transmethylation and transportation of amine. Meanwhile, the administration of M. officinalis can alleviate the kidney impairment induced by 'Kidney-yang deficiency syndrome'. PMID:24475714