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Sample records for cajan cajan germinadas

  1. Antiplasmodial constituents of Cajanus cajan.

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    Duker-Eshun, George; Jaroszewski, Jerzy W; Asomaning, William A; Oppong-Boachie, Francis; Brøgger Christensen, S

    2004-02-01

    Bioactivity-guided fractionation of extracts of roots and leaves of Cajanus cajan afforded 8 compounds: betulinic acid, biochanin A, cajanol, genistein and 2'-hydroxygenistein, longistylin A and C, and pinostrobin. The two stilbenes, longistylin A and C, and betulinic acid showed a moderately high in vitro activity against the chloroquine-sensitive Plasmodium falciparum strain 3D7. PMID:15022164

  2. Pigeonpea (Cajanus cajan L. Millsp.).

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    Sharma, Kiran Kumar; Sreelatha, Gopinath; Dayal, Sunitha

    2006-01-01

    Pigeonpea [Cajanus cajan (L.) Millsp.], also known as redgram, is one of the major grain legume (pulses) crops grown in the semiarid tropics (SAT) extending between 30 degrees N and 30 degrees S; it is the second most important food legume of India. It is cultivated in about 50 countries of Asia, Africa, and the Americas for a variety of uses (food, fodder, fuel wood, rearing lac insects, hedges, wind breaks, soil conservation, green manure, roofing, and so on). The constraints of enhancing its productivity include the damage caused by various fungi, bacteria, viruses, and insect pests. Conventional plant breeding methods have not been successful for the improvement of pigeonpea because of genetic variation and incompatibility among the wild varieties. Genetic engineering technology can therefore be used as an additional tool for the introduction of agronomically useful traits into established varieties. The development of plant transformation techniques has been a major breakthrough in overcoming constraints to achieve precision in genetic manipulation. The development of efficient plant regeneration protocols is a prerequisite for recombinant technology to carry out genetic transformation. This chapter describes an Agrobacterium-mediated transformation protocol for pigeonpea, a simple, efficient, and reproducible method that is applicable across diverse genotypes of pigeonpea. PMID:16988359

  3. A new isocoumarin from Cajanus cajan (Fabaceae).

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    Rodrigues, Virginia F; Oliveira, Rodrigo R; Vega, Maria Raquel G

    2014-04-01

    A new isocoumarin, 3-phenyl-8-hydroxy-6-methoxy-5-gamma,gamma-dimethylallyl-isocoumarin, named cajavilmina (1) and eight known compounds: a-amirenone (2), beta-amirenone (3), lupenone (4), 5-hydroxy-7-methoxydihydroflavone (5), longistilin C (6), 3-hydroxy-5-methoxystilbene (7), beta-sitosterol (8) and stigmasterol (9) were identified in a dichloromethane fraction from Cajanus cajan leaves. Structures were elucidated by analysis of spectral data, mainly those afforded by 1H, NOEDIFF and 13C NMR (1D and 2D NMR HMQC, HMBC and COSY) and mass spectra. PMID:24868865

  4. Biological activities and medicinal properties of Cajanus cajan (L Millsp.

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    Dilipkumar Pal

    2011-01-01

    Full Text Available Cajanus cajan (L Millsp. (Sanskrit: Adhaki, Hindi: Arhar, English: Pigeon pea, Bengali: Tur (family: Fabaceae is the most important grain legume crop of rain-fed agriculture in semi-arid tropics. It is both a food crop and a cover/forage crop with high levels of proteins and important amino acids like methionine, lysine and tryptophan. During the last few decades extensive studies have been carried out regarding the chemistry of C. cajan and considerable progress has been achieved regarding its biological activities and medicinal applications. This review article gives an overview on the biological activities of the compounds isolated, pharmacological actions and clinical studies of C. cajan extracts apart from its general details.

  5. Interspecific hybridization between Cajanus cajan and Cajanus cajanifolius

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    Venkatesan Thiruvengadam

    2007-01-01

    Full Text Available Interspecific hybridization between five Canajus cajan genotypes viz., ICPL 87, CORG 9302, CORG 5, TAT 93-47 and AS 46 and the wild species C. cajanifolius was attempted. Successful pod set was observed in direct crosses involvingC. cajan as ovule parent and C. cajanifolius as pollen parent while the reciprocal crosses failed to set pods. The only survivingF1 of ICPL 87 × C. cajanifolius was vigorous in growth and exhibited intermediate leaf and flower morphology with predominanceof C. cajanifolius plant type. Chromosome pairing was rather irregular in the F1 hybrid with loose pairing, univalents,quadrivalents and abnormalities like laggards, stickiness, bridges, precocious separation and non-synchronization of thegenomes. Pollen fertility of the hybrid was low and occurrence of triads was noticed. The partial homology coupled with seedset suggested the possibility of incorporating traits of economic importance such as pest resistance from C. cajanifolius intoC. cajan.

  6. Cajachalcone: An Antimalarial Compound from Cajanus cajan Leaf Extract.

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    Ajaiyeoba, E O; Ogbole, O O; Abiodun, O O; Ashidi, J S; Houghton, P J; Wright, C W

    2013-01-01

    Cajanus cajan L, a member of the family Fabaceae, was identified from the Nigerian antimalarial ethnobotany as possessing antimalarial properties. The bioassay-guided fractionation of the crude methanol extract of C. cajan leaves was done in vitro using the multiresistant strain of Plasmodium falciparum (K1) in the parasite lactate dehydrogenase assay. Isolation of compound was achieved by a combination of chromatographic techniques, while the structure of the compound was elucidated by spectroscopy. This led to the identification of a cajachalcone, 2',6'-dihydroxy-4-methoxy chalcone, as the biologically active constituent from the ethyl acetate fraction. Cajachalcone had an IC50 value of 2.0  μ g/mL (7.4  μ M) and could be a lead for anti-malarial drug discovery. PMID:23970954

  7. Cajachalcone: An Antimalarial Compound from Cajanus cajan Leaf Extract

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    E. O. Ajaiyeoba

    2013-01-01

    Full Text Available Cajanus cajan L, a member of the family Fabaceae, was identified from the Nigerian antimalarial ethnobotany as possessing antimalarial properties. The bioassay-guided fractionation of the crude methanol extract of C. cajan leaves was done in vitro using the multiresistant strain of Plasmodium falciparum (K1 in the parasite lactate dehydrogenase assay. Isolation of compound was achieved by a combination of chromatographic techniques, while the structure of the compound was elucidated by spectroscopy. This led to the identification of a cajachalcone, 2′,6′-dihydroxy-4-methoxy chalcone, as the biologically active constituent from the ethyl acetate fraction. Cajachalcone had an IC50 value of 2.0 μg/mL (7.4 μM and could be a lead for anti-malarial drug discovery.

  8. Identification of metabolites from an active fraction of Cajanus cajan seeds by high resolution mass spectrometry.

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    Tekale, Satishkumar S; Jaiwal, Bhimrao V; Padul, Manohar V

    2016-11-15

    Antioxidants are important food additives which prolong food storage due to their protective effects against oxidative degradation of foods by free radicals. However, the synthetic antioxidants show toxic properties. Alternative economical and eco-friendly approach is screening of plant extract for natural antioxidants. Plant phenolics are potent antioxidants. Hence, in present study Cajanus cajan seeds were analyzed for antioxidant activity, Iron chelating activity and total phenolic content. The antioxidant activity using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay showed 71.3% inhibition and 65.8% Iron chelating activity. Total 37 compounds including some short peptides and five major abundant compounds were identified in active fraction of C. cajan seeds. This study concludes that C. cajan seeds are good source of antioxidants and Iron chelating activity. Metabolites found in C. cajan seeds which remove reactive oxygen species (ROS), may help to alleviate oxidative stress associated dreaded health problem like cancer and cardiovascular diseases. PMID:27283694

  9. Cajanus cajan- a source of PPARγ activators leading to anti-inflammatory and cytotoxic effects.

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    Schuster, Roswitha; Holzer, Wolfgang; Doerfler, Hannes; Weckwerth, Wolfram; Viernstein, Helmut; Okonogi, Siriporn; Mueller, Monika

    2016-09-14

    Cajanus cajan is an important legume crop in the human diet in many parts of the world. Due to its pharmacological properties, C. cajan is, moreover, used in traditional medicine for treating skin diseases, diabetes, inflammatory disorders and various other dysfunctions. In this study, we focused on the role of peroxisome proliferator-activated receptor gamma (PPARγ) as a potential therapeutic target of Cajanus cajan and its main compounds for the treatment of cancer, inflammation and inflammation-related disorders. The anti-inflammatory potential of C. cajan and its bioactive compounds and their cytotoxicity on the human cervical adenocarcinoma cell line HeLa, the human colorectal adenocarcinoma cell line CaCo-2 and the human breast adenocarcinoma cell line MCF-7 were elucidated. C. cajan and its compounds exerted significant anti-inflammatory activity on lipopolysaccharide-stimulated macrophages, showed good cytotoxic effects on the 3 different cancer cell lines and proved PPARγ activity in vitro. The main active compounds were orientin, pinostrobin and vitexin. Cajaninstilbene acid and pinosylvin monomethylether were identified as novel PPARγ activators. Based on these data, C. cajan provides excellent beneficial medicinal attributes and may be used as a potential food or a pharmaceutical supplement. PMID:27603115

  10. Guandu bean (Cajanus cajan (L. Millsp. on tropical forest restoration/ Feijão guandu (Cajanus cajan (L. Millsp. na restauração de florestas tropicais

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    Efraim Rodrigues

    2007-07-01

    Full Text Available This work aims to evaluate alternative models in forest restorations by means of agroforestry systems, where a leguminous species is incorporated to the system. The hypothesis is that guandu bean Cajanus cajan (L. Millsp., in combination and intercropped with native species, can promote restoration by decreasing mortality and increasing tree basal area and height. Cajanus cajan was planted in line with the forest seedlings in 2 x 4 m spacing. Four different treatments were analyzed; 1 control, with no Cajanus cajan ; 2 one seedling of Cajanus cajan between the forest seedlings in the plantation line; 3 two seedlings of Cajanus cajan between the forest seedlings in the plantation line; and 4 full harvest Cajanus cajan six months after plot implementation. In each treatment, 100 native trees (Fifty pioneer, and fifty non pioneer trees were measured and evaluated in mortality rates, height, and basal diameter. Guandu planting reduced pioneer mortality, increased basal area, and tree height of all species. In spite of the mortality was not affected by guandu density, the density of one plant of guandu was associated with larger basal area and tree average height. The mortality reduction of non pioneers on the absence of guandu trees was interpreted as a response of antropic pioneers to the increased solar radiation. This result points to the differences in tree behavior between fertile gaps (on which the classification on sucessional stages was based and degraded areas. The positive effects of guandu planting recommend its use for the ecological restoration cost reduction.O objetivo deste trabalho é avaliar formas alternativas de restauração de áreas degradadas, utilizando sistemas agroflorestais com uma espécie leguminosa incorporada ao sistema. A hipótese é que o feijão guandu (Cajanus cajan (L. Millsp. possa auxiliar o processo de restauração, diminuindo a mortalidade e aumentando a altura e área basal das árvores. O feijão guandu

  11. Characterisation of a novel enterobacteria phage, CAjan, isolated from rat faeces.

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    Carstens, Alexander B; Kot, Witold; Lametsch, Rene; Neve, Horst; Hansen, Lars H

    2016-08-01

    In this study, we describe the isolation and characterisation of the novel enterobacteria phage CAjan. This phage belongs to the order Caudovirales and the family Siphoviridae. The phage possesses a linear, double-stranded DNA genome consisting of 59,670 bp with a G+C content of 44.7 % and 91 predicted open reading frames (ORFs). Putative functions were assigned to 39 of the ORFs (37.4 %). The phage structural genes were furthermore functionally characterised by LC MS/MS. CAjan, together with Escherichia phage Seurat and Escherichia phage slur01, represent a novel and genetically distinct clade of Siphoviridae phages that could be considered to constitute a new phage genus. Despite limited sequence similarity, the phages in this group share a number of other common features, including genome structure and the presence of queuosine biosynthesis genes. PMID:27231007

  12. OLIGOSACCHARIDE LEVELS IN IMMATURE AND NATURE SEEDS FROM SEVERAL VARIETIES OF PIGEON PEAS (Cajanus cajan)

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    Jairo Osvaldo CAZETTA; Hugo Candido SILVA; Gilberto Leite BRAGA; Roberto de CARVALHO

    2009-01-01

    ABSTRACT: Oligosaccharide level were evaluated in immature and nature seeds from different varieties of pigeon peas (Cajanus cajan) and compared to those of common beans (Phaseolus vulgaris) and peas (Pisum sativum): The effect of seed processing by soaking and cooking was also determined. Immature pigeon peas seeds presented low oligosaccharide levels when compared to mature pigeon pea seeds or to pea seeds, thus representing a good alternative for human consumption....

  13. Genetic Patterns of Domestication in Pigeonpea (Cajanus cajan (L.) Millsp.) and Wild Cajanus Relatives

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    Mulualem T Kassa; Varma Penmetsa, R.; Noelia Carrasquilla-Garcia; Sarma, Birinchi K.; Subhojit Datta; Hari D. Upadhyaya; Rajeev K Varshney; Eric J B von Wettberg; Cook, Douglas R.

    2012-01-01

    Pigeonpea (Cajanus cajan) is an annual or short-lived perennial food legume of acute regional importance, providing significant protein to the human diet in less developed regions of Asia and Africa. Due to its narrow genetic base, pigeonpea improvement is increasingly reliant on introgression of valuable traits from wild forms, a practice that would benefit from knowledge of its domestication history and relationships to wild species. Here we use 752 single nucleotide polymorphisms (SNPs) de...

  14. Efficacy of micronutrients in influencing growth behavior of Rhizobium of Pigeonpea (Cajanus cajan L. [Millsp.])

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    Sonam Paliya; A.N. Tikle; Tessy Thomas

    2014-01-01

    he present investigation was conducted to observe the effect of various concentrations (0.1 to 1.5 gm/100ml) of micronutrients (Zn, Mn, B, Cu, Mo, Fe) with yeast extract mannitol media on agriculturally important Rhizobium species isolated from root nodules of Cajanus cajan and to determine the effect of multi-micronutrient yeast extract mannitol media prepared of maximum growth concentrations of micronutrients on rhizobial growth. Concentrati...

  15. Antioxidant Activities of Extracts and Main Components of Pigeonpea [Cajanus cajan (L.) Millsp.] Leaves

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    Xiao-Lei Liu; Yung-Husan Chen; Fang-Rong Chang; Yuan-Gang Zu; Yu-Jie Fu; Kuang Fu; Nan Wu; Cheng-Bo Gu; Wei Liu; Yu Kong

    2009-01-01

    Antioxidant activities of the aqueous and ethanol extracts of pigeonpea [Cajanus cajan (L.) Millsp.] leaves, as well as petroleum ether, ethyl acetate, n-butanol and water fractions and the four main compounds separated from the ethanol extract, i.e. cajaninstilbene acid (3-hydroxy-4-prenylmethoxystilbene-2-carboxylic acid), pinostrobin, vitexin and orientin, were examined by a DPPH radical-scavenging assay and a β-carotene-linoleic acid test. In the DPPH system, the antioxidant activity of t...

  16. Evaluation of Cajanus cajan (pigeon pea) for phytoremediation of landfill leachate containing chromium and lead.

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    Jerez Ch, José A; Romero, Rosaura M

    2016-11-01

    Landfill leachates containing heavy metals are important contaminants and a matter of great concern due to the effect that they might have on ecosystems. We evaluated the use of Cajanus cajan to remove chromium and lead from landfill leachates. Eight-week-old plants were submitted to varied tests to select the experimental conditions. Water assays with a solution (pH 6) containing leachate (25% v/v) were selected; the metals were added as potassium dichromate and lead (II) nitrate salts. Soil matrices that contained leachate (30% v/v) up to field capacity were used. For both water and soil assays, the metal concentrations were 10 mg kg(-1). C. cajan proved able to remove 49% of chromium and 36% of lead, both from dilute leachate. The plants also removed 34.7% of chromium from irrigated soil, but were unable to decrease the lead content. Removal of nitrogen from landfill leachate was also tested, resulting in elimination of 85% of ammonia and 70% of combined nitrite/nitrate species. The results indicate that C. cajan might be an effective candidate for the rhizofiltration of leachates containing chromium and lead, and nitrogen in large concentrations. PMID:27196815

  17. Morpho-anatomical and growth alterations induced by arsenic in Cajanus cajan (L.) DC (Fabaceae).

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    Pita-Barbosa, Alice; Gonçalves, Elton Carvalho; Azevedo, Aristéa Alves

    2015-08-01

    Arsenic (As) is a toxic element to most organisms. Studies investigating anatomic alterations due to As exposure in plants are scarce but of utmost importance to the establishment of environmental biomonitoring techniques. So, this study aimed to investigate the effects of As on the development and initial root growth in Cajanus cajan (Fabaceae), characterize and quantify the possible damages, evaluate genotoxic effects, and identify structural markers to be used in environmental bioindication. Plants were exposed hydroponically to 0.5, 1.0, 1.5, and 2.0 mg As L(-1), as sodium arsenate. Growth parameters were measured, and in the end of the exposure, root samples were analyzed for qualitative and quantitative anatomical alterations. Arsenic genotoxicity was evaluated through analysis of the mitotic index in the root apex. Compared to the control, As-treated seedlings showed an altered architecture, with significantly decreased root length (due to the lower mitotic index in the apical meristem and reduced elongation of parenchyma cells) with darkened color, and abnormal development of the root cap. A significant increase in vascular cylinder/root diameter ratio was also detected, due to the reduction of the cellular spaces in the cortex. The secondary xylem vessel elements were reduced in diameter and had sinuous walls. The severest damage was visible in the ramification zone, where uncommon division planes of phellogen and cambium cells and disintegration of the parenchyma cells adjacent to lateral roots were observed. The high sensibility of C. cajan to As was confirmed, since it caused severe damages in root growth and anatomy. The main structural markers for As toxicity were the altered root architecture, with the reduction of the elongation zone and increase of ramification zone length, and the root primordia retained within the cortex. Our results show a new approach about As toxicity and indicate that C. cajan is a promising species to be used for

  18. In vitro antioxidant and pharmacognostic studies of leaf extracts of cajanus cajan (l. millsp

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    B Mahitha

    2015-01-01

    Full Text Available Cajanus cajan (L. Millsp is one of the second most dietary legume crops. The leaf extracts may be used as a potential source of natural antioxidant. The ash values, extractive values, total phenolic and flavonoid content, in vitro antioxidant activity of various leaf extracts as well as anatomical investigation of Cajanus cajan were carried out. Physicochemical parameters such as total, acid-insoluble and water-soluble ash values and moisture content of the leaf powder of C. cajan were found to be 9.50%, 1.40 g/100 g, 4.15 g/100 g drug and 6.72%, respectively. Percent yield of acetone, aqueous, ethanol, ethyl acetate and chloroform leaf extracts were 9.0, 10.6, 13.75, 8.7 and 5.8 g/100 g, respectively. Significant amount of phenolic and flavonoid content were observed. The results of the antioxidant activity were found to be concentration-dependent. The IC 50 values for DPPH assay determined for aqueous and ethanol extracts were 0.69 and 0.79 mg/ml, respectively. Reducing power is increased with increasing amount of concentration in both aqueous and ethanol leaf extracts. The highest hydroxyl radical scavenging activity reached up to 83.67% in aqueous and 78.75% in ethanol extracts and in phosphomolybdenum assay the aqueous extract showed strong antioxidant capacity up to 55.97 nM gallic acid equivalents/g. It was found that the aqueous extract possessed highest antioxidant activity in all the assays tested.The antioxidant characteristics of leaf extracts are possibly because of the presence of polyphenols. Microscopic study showed the presence of collenchyma, fibres, xylem, phloem, epidermis, trichomes, palisade tissue, basal sheath, pith and cortex in leaf, petiole and pulvinus.

  19. In vitro Antioxidant and Pharmacognostic Studies of Leaf Extracts of Cajanus cajan (L.) Millsp.

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    Mahitha, B; Archana, P; Ebrahimzadeh, Md H; Srikanth, K; Rajinikanth, M; Ramaswamy, N

    2015-01-01

    Cajanus cajan (L.) Millsp is one of the second most dietary legume crops. The leaf extracts may be used as a potential source of natural antioxidant. The ash values, extractive values, total phenolic and flavonoid content, in vitro antioxidant activity of various leaf extracts as well as anatomical investigation of Cajanus cajan were carried out. Physicochemical parameters such as total, acid-insoluble and water-soluble ash values and moisture content of the leaf powder of C. cajan were found to be 9.50%, 1.40 g/100 g, 4.15 g/100 g drug and 6.72%, respectively. Percent yield of acetone, aqueous, ethanol, ethyl acetate and chloroform leaf extracts were 9.0, 10.6, 13.75, 8.7 and 5.8 g/100 g, respectively. Significant amount of phenolic and flavonoid content were observed. The results of the antioxidant activity were found to be concentration-dependent. The IC50 values for DPPH assay determined for aqueous and ethanol extracts were 0.69 and 0.79 mg/ml, respectively. Reducing power is increased with increasing amount of concentration in both aqueous and ethanol leaf extracts. The highest hydroxyl radical scavenging activity reached up to 83.67% in aqueous and 78.75% in ethanol extracts and in phosphomolybdenum assay the aqueous extract showed strong antioxidant capacity up to 55.97 nM gallic acid equivalents/g. It was found that the aqueous extract possessed highest antioxidant activity in all the assays tested. The antioxidant characteristics of leaf extracts are possibly because of the presence of polyphenols. Microscopic study showed the presence of collenchyma, fibres, xylem, phloem, epidermis, trichomes, palisade tissue, basal sheath, pith and cortex in leaf, petiole and pulvinus. PMID:26009649

  20. HCH, endosulfan, and fluvalinate residue behavior in pigeonpea (Cajanus cajan L. Millsp)

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    Mukherjee, I.; Gopal, M.; Yaduraju, N.T. (Indian Agricultural Research Inst., New Delhi (India))

    1992-01-01

    Pigeonpea (Cajanus cajan L. Millsp) is one of the major pulse crop of India. The loss of pigeonpea crop due to pod and foliage pests is significant, the major pests being pod fly, hairy caterpillar, aphids, white fly, plume moth borer, leaf caterpillar and jassids. It is imperative to save every grain by chemical control methods but these toxicants should not leave unusually high residues on the edible parts. In this paper the authors report the residue behavior of three different insecticides namely, hexachlorocyclohexane, endosulfan and fluvalinate on this crop.

  1. Pinostrobin from Cajanus cajan (L.) Millsp. inhibits sodium channel-activated depolarization of mouse brain synaptoneurosomes.

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    Nicholson, Russell A; David, Laurence S; Pan, Rui Le; Liu, Xin Min

    2010-10-01

    This investigation focuses on the in vitro neuroactive properties of pinostrobin, a substituted flavanone from Cajanus cajan (L.) Millsp. of the Fabaceae family. We demonstrate that pinostrobin inhibits voltage-gated sodium channels of mammalian brain (IC(50)=23 µM) based on the ability of this substance to suppress the depolarizing effects of the sodium channel-selective activator veratridine in a synaptoneurosomal preparation from mouse brain. The resting membrane potential of synaptoneurosomes was unaffected by pinostrobin. The pharmacological profile of pinostrobin resembles that of depressant drugs that block sodium channels. PMID:20472040

  2. Hepatoprotective effect of Caesalpinia gilliesii and Cajanus cajan proteins against acetoaminophen overdose-induced hepatic damage.

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    Rizk, Maha Z; Aly, Hanan F; Abo-Elmatty, Dina M; Desoky, M M; Ibrahim, N; Younis, Eman A

    2016-05-01

    This study aims to evaluate two proteins derived from the seeds of the plants Cajanus cajan (Leguminosae) and Caesalpinia gilliesii (Leguminosae) for their abilities to ameliorate the toxic effects of chronic doses of acetoaminphen (APAP) through the determination of certain biochemical parameters including liver marker enzymes: alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and total bilirubin. Also, total protein content and hepatic marker enzyme, lactate dehydrogenase were studied. Moreover, liver antioxidants, glutathione (GSH), nitric oxide, and lipid peroxides were determined in this study. Hepatic adenosine triphosphatase (ATPase), adenylate energy charge (ATP, adenosine diphosphate, adenosine monophosphate, and inorganic phosphate), and phosphate potential, serum interleukin-6, tumor necrosis factor-α, and myeloperoxidase were also examined in the present study. On the other hand, histopathological examination of intoxicated and liver treated with both proteins was taken into consideration. The present results show disturbances in all biochemical parameters and hepatic toxicity signs including mild vascular congestion, moderate inflammatory changes with moderate congested sinusoids, moderate nuclear changes (pyknosis), moderate centrilobular necrosis, fatty changes, nuclear pyknosis vascular congestion, and change in fatty centrilobular necrosis liver. Improvement in all biochemical parameters studied was noticed as a result of treatment intoxicated liver with C. gilliesii and C. cajan proteins either paracetamol with or post paracetamol treatment. These results were documented by the amelioration signs in rat's hepatic architecture. Thus, both plant protein extracts can upregulate and counteract the inflammatory process, minimize damage of the liver, delay disease progression, and reduce its complications. PMID:24414985

  3. Genetic patterns of domestication in pigeonpea (Cajanus cajan (L.) Millsp.) and wild Cajanus relatives.

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    Kassa, Mulualem T; Penmetsa, R Varma; Carrasquilla-Garcia, Noelia; Sarma, Birinchi K; Datta, Subhojit; Upadhyaya, Hari D; Varshney, Rajeev K; von Wettberg, Eric J B; Cook, Douglas R

    2012-01-01

    Pigeonpea (Cajanus cajan) is an annual or short-lived perennial food legume of acute regional importance, providing significant protein to the human diet in less developed regions of Asia and Africa. Due to its narrow genetic base, pigeonpea improvement is increasingly reliant on introgression of valuable traits from wild forms, a practice that would benefit from knowledge of its domestication history and relationships to wild species. Here we use 752 single nucleotide polymorphisms (SNPs) derived from 670 low copy orthologous genes to clarify the evolutionary history of pigeonpea (79 accessions) and its wild relatives (31 accessions). We identified three well-supported lineages that are geographically clustered and congruent with previous nuclear and plastid sequence-based phylogenies. Among all species analyzed Cajanus cajanifolius is the most probable progenitor of cultivated pigeonpea. Multiple lines of evidence suggest recent gene flow between cultivated and non-cultivated forms, as well as historical gene flow between diverged but sympatric species. Evidence supports that primary domestication occurred in India, with a second and more recent nested population bottleneck focused in tropical regions that is the likely consequence of pigeonpea breeding. We find abundant allelic variation and genetic diversity among the wild relatives, with the exception of wild species from Australia for which we report a third bottleneck unrelated to domestication within India. Domesticated C. cajan possess 75% less allelic diversity than the progenitor clade of wild Indian species, indicating a severe "domestication bottleneck" during pigeonpea domestication.

  4. Antioxidant status of pigeon pea, Cajanus cajan in the presence of endosulfan stress.

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    Mathad, Pratima; Siddaling, N C

    2009-05-01

    Antioxidative status study was made in cotyledons of 7days old as well as in leaf and stem tissues of 30 and 60 days old pigeon pea (Cajanus cajan) namely Asha and Maruti subjected to different doses of endosulfan in the range 0.1-1.0%. The results revealed that the activities of the antioxidative enzymes and the antioxidant contents such as the super oxide dismutase (SOD), peroxidase (POD), reducing power (RP), ascorbic acid (AsA) and total phenols (TP) increased with increase in the concentrations of endosulfan in different parts of the plants in both the varieties. It was interesting to note that the increase in the antioxidative enzymes and the antioxidant contents were higher in leaves than in stem and cotyledons in both the plant varieties. The Asha showed lower activity of SOD and higher activity of POD than the Maruti. The RP and AsA contents were higher whereas the TP content was lower in Asha than Maruti. The observed variations in the activities of the oxidative enzymes and the antioxidant contents of the plants treated with the varying concentration of endosulfan indicated that the antioxidative system in the plants plays a fundamental role in minimizing the deleterious effects of the oxidative stress in the two varieties of Cajanus cajan.

  5. Potencial de Cajanus cajan e Crotalaria spectabilis parafitorremediação: absorção de arsênio e respostas antioxidativas Potential of Cajanus cajan and Crotalaria spectabilis for phytoremediation: arsenic absorption and antioxidative responses

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    Rafaella Teles Arantes Felipe

    2009-04-01

    Full Text Available Neste estudo, avaliaram-se os efeitos tóxicos do arsênio (As sobre o crescimento e atividade de enzimas antioxidativas dismutase do superóxido (SOD, catalase (CAT, peroxidases (POX, peroxidase do ascorbato peroxidase (APX e glutationa redutase (GR em plantas de Cajanus cajan e Crotalaria spectabili. Plantas das duas espécies foram expostas a diferentes concentrações de As, em solução nutritiva de Hoagland, pH 6,5. A taxa de crescimento relativo (TCR das duas espécies reduziu-se com o aumento da concentração de As na solução nutritiva, sendo de forma mais intensa em C. cajan. A concentração de As na solução capaz de reduzir em 50% a TCR de C. cajan foi de 0,93 mg L-1, enquanto em C. spectabilis foi de 4,80 mg L-1. As duas espécies apresentaram reduzida translocação de As para a parte aérea, sendo em raízes de C. spectabilis observada a maior concentração desse elemento. C. cajan exposto ao As teve as atividades das enzimas CAT, POX e APX reduzidas, ao contrário do que ocorreu em C. spectabilis. Esta espécie, quando submetida ao As, apresentou incremento na atividade de todas as enzimas avaliadas. A maior tolerância ao As observada em C. spectabilis pode estar relacionada à maior capacidade em reter o As nas raízes e à indução das enzimas SOD, CAT, POX, APX e GR. Dessa forma, C. spectabilis é uma espécie com potencial para utilização em programas de revegetação de áreas contaminadas com As.The toxic effects of Arsenic (As on the growth and activities of the antioxidative enzymes superoxide dismutase (SOD, catalase (CAT, peroxidase (POX, ascorbate peroxidase (APX and glutathione reductase (GR of Cajanus cajan and Crotalaria spectabilsi were studied. AQUI Plants of the two species were exposed to different As concentrations in Hoagland's nutrient solution, pH 6.5. The relative growth rate (RGR of the two species reduced with the increase in As concentration in the nutrient solution, especially in C. cajan

  6. Structural and functional studies on urease from pigeon pea (Cajanus cajan).

    Science.gov (United States)

    Balasubramanian, Anuradha; Durairajpandian, Vishnuprabu; Elumalai, Sagadevan; Mathivanan, Narayanasamy; Munirajan, Arasambattu Kannan; Ponnuraj, Karthe

    2013-07-01

    Urease is an enzyme that catalyzes the hydrolysis of urea, forming ammonia and carbon dioxide, and is found in plants, microorganisms and invertebrates. Although plant and bacterial ureases are closely related at amino acid and at the structural level, the insecticidal activity is seen only in the plant ureases. In contrast, both plant and bacterial ureases exhibit antifungal activity. These two biological properties are independent of its ureolytic activity. However, till date the mechanism(s) behind the insecticidal and fungicidal activity of ureases are not clearly understood. Here we report the crystal structure of pigeon pea urease (PPU, Cajanus cajan) which is the second structure from the plant source. We have deduced the amino acid sequence of PPU and also report here studies on its stability, insecticidal and antifungal activity. PPU exhibits cellulase activity. Based on the structural analysis of PPU and docking studies with cellopentoase we propose a possible mechanism of antifungal activity of urease. PMID:23624166

  7. Comparative analysis of genome-wide Mlo gene family in Cajanus cajan and Phaseolus vulgaris.

    Science.gov (United States)

    Deshmukh, Reena; Singh, V K; Singh, B D

    2016-04-01

    The Mlo gene was discovered in barley because the mutant 'mlo' allele conferred broad-spectrum, non-race-specific resistance to powdery mildew caused by Blumeria graminis f. sp. hordei. The Mlo genes also play important roles in growth and development of plants, and in responses to biotic and abiotic stresses. The Mlo gene family has been characterized in several crop species, but only a single legume species, soybean (Glycine max L.), has been investigated so far. The present report describes in silico identification of 18 CcMlo and 20 PvMlo genes in the important legume crops Cajanus cajan (L.) Millsp. and Phaseolus vulgaris L., respectively. In silico analysis of gene organization, protein properties and conserved domains revealed that the C. cajan and P. vulgaris Mlo gene paralogs are more divergent from each other than from their orthologous pairs. The comparative phylogenetic analysis classified CcMlo and PvMlo genes into three major clades. A comparative analysis of CcMlo and PvMlo proteins with the G. max Mlo proteins indicated close association of one CcMlo, one PvMlo with two GmMlo genes, indicating that there was no further expansion of the Mlo gene family after the separation of these species. Thus, most of the diploid species of eudicots might be expected to contain 15-20 Mlo genes. The genes CcMlo12 and 14, and PvMlo11 and 12 are predicted to participate in powdery mildew resistance. If this prediction were verified, these genes could be targeted by TILLING or CRISPR to isolate powdery mildew resistant mutants. PMID:26961357

  8. Comparative analysis of genome-wide Mlo gene family in Cajanus cajan and Phaseolus vulgaris.

    Science.gov (United States)

    Deshmukh, Reena; Singh, V K; Singh, B D

    2016-04-01

    The Mlo gene was discovered in barley because the mutant 'mlo' allele conferred broad-spectrum, non-race-specific resistance to powdery mildew caused by Blumeria graminis f. sp. hordei. The Mlo genes also play important roles in growth and development of plants, and in responses to biotic and abiotic stresses. The Mlo gene family has been characterized in several crop species, but only a single legume species, soybean (Glycine max L.), has been investigated so far. The present report describes in silico identification of 18 CcMlo and 20 PvMlo genes in the important legume crops Cajanus cajan (L.) Millsp. and Phaseolus vulgaris L., respectively. In silico analysis of gene organization, protein properties and conserved domains revealed that the C. cajan and P. vulgaris Mlo gene paralogs are more divergent from each other than from their orthologous pairs. The comparative phylogenetic analysis classified CcMlo and PvMlo genes into three major clades. A comparative analysis of CcMlo and PvMlo proteins with the G. max Mlo proteins indicated close association of one CcMlo, one PvMlo with two GmMlo genes, indicating that there was no further expansion of the Mlo gene family after the separation of these species. Thus, most of the diploid species of eudicots might be expected to contain 15-20 Mlo genes. The genes CcMlo12 and 14, and PvMlo11 and 12 are predicted to participate in powdery mildew resistance. If this prediction were verified, these genes could be targeted by TILLING or CRISPR to isolate powdery mildew resistant mutants.

  9. COMPARATIVE STUDIES ON ANTHELMINTIC ACTIVITY OF LEAF EXTRACT OF MUSA ACUMINATE COLLA AND CAJANUS CAJAN (LINN. LEAF EXTRACT

    Directory of Open Access Journals (Sweden)

    Bhakta Tejendra

    2013-01-01

    Full Text Available Methanolic extract of leaf of Musa acuminate colla and seed of Cajanus cajan (Linn. were taken for the examination of anthelmintic activity on Indian earthworm (Pheretima posthuma. Various concentrations (10mg/ml, 20mg/ml, 30 mg/ml of both plant extract were tested and results were expressed in terms of time of paralysis and time of death of worms. Albendazole of same concentration as like as plant extracts was taken as reference standard and normal saline solution as control. Paralysis time can be determined by vigorous shaking when no movement was observed. When whitish substances were secreted from the body that one was termed as death time. Both the extracts show significant anthelmintic activity but among these two Musa acuminate colla showed more anthelmintic activity than Cajanus cajan (Linn.. Future scope involves isolation of active constituents responsible for this action.

  10. (+)- and (-)-Cajanusine, a pair of new enantiomeric stilbene dimers with a new skeleton from the leaves of Cajanus cajan.

    Science.gov (United States)

    Li, Xiao-Long; Zhao, Bing-Xin; Huang, Xiao-Jun; Zhang, Dong-Mei; Jiang, Ren-Wang; Li, Ying-Jie; Jian, Yu-Qing; Wang, Ying; Li, Yao-Lan; Ye, Wen-Cai

    2014-01-01

    A pair of new enantiomeric stilbene dimers, (+)- and (-)-cajanusine [(+)-1 and (-)-1], with a unique coupling pattern were isolated from the leaves of Cajanus cajan . Their structures including absolute configurations were elucidated on the basis of comprehensive spectroscopic and single-crystal X-ray diffraction analyses, as well as CD calculations. The plausible biogenetic pathway of 1 was also proposed. Additionally, (±)-1, (+)-1, and (-)-1 exhibited inhibitory activities on the growth of human hepatocellular carcinoma cells. PMID:24295169

  11. Growth and Nutrient Utilization in Kids Fed Expander-extruded Complete Feed Pellets Containing Red Gram (Cajanus cajan) Straw

    OpenAIRE

    Reddy, P. B.; Reddy, T. J.; Reddy, Y. R.

    2012-01-01

    A growth and digestibility study was conducted using Osmanabadi goat male kids by feeding complete diets in the form of mash or expander extruded pellets containing different levels of red gram (Cajanus cajan) straw (RGS). Two iso-nitrogenous complete diets were prepared by incorporating RGS at 35% and 50% levels. Half the quantity of each complete mash feed was then converted into pellets through expander extruder processing. Thirty two kids of 4 to 5 months age were divided into four groups...

  12. PATTERN OF PLANT REGENERATION FROM SHOOT TIP EXPLANTS OF PIGEONPEA (CAJANUS CAJAN L MILLLSP) VAR LRG-41

    OpenAIRE

    T. Raghavendra; P. Sudhakar

    2014-01-01

    An efficient direct shoot bud differentiation and multiple shoot induction from shoot tip explants of pigeon pea (Cajanus cajan L.) has been achieved. The frequency of shoot bud regeneration was influenced by the type of explants, genotype and concentrations of cytokinin. Explants viz. shoot tip isolated from 10 day old seedlings showed better explants response Explants were cultured on Murashige and skoog (MS) medium augmented with different concentrations of BAP and NAA. Among the various c...

  13. Pinostrobin and Cajanus lactone isolated from Cajanus cajan (L.) leaves inhibits TNF-α and IL-1β production: in vitro and in vivo experimentation.

    Science.gov (United States)

    Patel, Neeraj K; Bhutani, Kamlesh K

    2014-06-15

    The tumor necrosis factor alpha (TNF-α) and interleukin 1 beta (IL-1β) inhibitory activities of Cajanus cajan (leaves) crude methanolic extract, its fractions and its phytochemical constituents were evaluated in lipopolysaccharide (LPS) stimulated RAW 264.7 and J774A.1 cells. Phytochemical investigation of the active ethyl acetate (CCE) and n-butanol (CCB) fractions of C. cajan L. leaves yielded 14 compounds. It was observed that both pinostrobin (9) and cajanus lactone (4) were found to be most active in inhibiting TNF-α (IC50cajanus lactone (4) were found to reduce LPS-induced TNF-α levels by 48.6% and 55.0% respectively and IL-1β levels by 53.1% and 41.8% respectively in Sprague Dawley (SD) rats. These findings suggest that C. cajan L. leaves can be developed as an effective herbal remedy for the treatment and prevention of inflammation or associated ailments. PMID:24680612

  14. 木豆新品种'CAF7'%A New Cajanus cajan Cultivar'CAF7'

    Institute of Scientific and Technical Information of China (English)

    刘秀贤; 李正红; 马宏; 万友名; 谷勇; 李立; 梁宁

    2011-01-01

    ‘CAF7’ is a new cultivar of Cajanus cajan, bred from endemic pigeonpea cultivars of Jingdong, Yunnan Province. Single plant selection and group selection were applied into the breeding course. Its growth period ranges 181 - 190 days and average number of pod per plant is (863.9 ± 354.5);Each pod contains (4.7 ± 0.4) beans; 100-seed weight is (7.74 ± 0.42) g; Total seed weight per plant is up to ( 83.20 ± 63.40) g.%'CAF7'是运用单株选择和集团选择育种手段,从云南景东县地方栽培品种中选育出的木豆新品种.生育期181~190 d,平均单株荚数(863.9±354.5)个,每荚粒数(4.7 4±0.4)粒,种子百粒质量(7.744±0.42)g,单株粒质量(83.20±63.40)g.

  15. Determination by flow cytometry polyploidy inducing-capacity of colchicine in Cajanus cajan (L.) Mill sp.

    Science.gov (United States)

    Udensi, O U; Ontui, V

    2013-07-01

    The need to optimize flow cytometric analysis for the determination of ploidy level is a worthwhile venture to precisely know at what concentration of a mutagen and at what time of exposure polyploidy could be induced. Flow cytometry was used to determine the polyploidy inducing-capacity of colchicine in pigeon pea (Cajanus cajan (L.) Mill sp). Seeds of pigeon pea were soaked in three different concentrations of colchicine-5 mg, 10 and 15 mg L(-1) for 24, 48 and 72 h, respectively, while the control group was soaked in water. Treated seeds and those from the control were planted in a greenhouse using a Completely Randomized Design (CRD). Results show that colchicine induced tetraploids (4n) and mixoploids (2n+ 4n) as the concentration of colchicine increased and soaking duration. Days to seedling emergence increased as concentration of colchicine and duration of soaking increased while germination rate decreased proportionately with the increase in colchicine concentration and soaking duration but did not significantly affect percentage seedling survival. Explicitly, colchicine has the capacity of inducing polyploidy; especially tetraploids on the seeds of pigeon pea, which obviously could be harnessed for further breeding and improvement of the pigeon pea. PMID:24505986

  16. A microdroplet cell culture based high frequency somatic embryogenesis system for pigeonpea, Cajanus cajan (L.) Millsp.

    Science.gov (United States)

    Kumar, Nagan Udhaya; Gnanaraj, Muniraj; Sindhujaa, Vajravel; Viji, Maluventhen; Manoharan, Kumariah

    2015-09-01

    A protocol for high frequency production of somatic embryos was worked out in pigeonpea, Cajanus cajan (L.) Millsp. The protocol involved sequential employment of embryogenic callus cultures, low density cell suspension cultures and a novel microdroplet cell culture system. The microdroplet cell cultures involved culture of a single cell in 10 μI of Murashige and Skoog's medium supplemented with phytohormones, growth factors and phospholipid precursors. By employing the microdroplet cell cultures, single cells in isolation were grown into cell clones which developed somatic embryos. Further, 2,4-dichlorophenoxyacetic acid, kinetin, polyethylene glycol, putrescine, spermine, spermidine, choline chloride, ethanolamine and LiCl were supplemented to the low density cell suspension cultures and microdroplet cell cultures to screen for their cell division and somatic embryogenesis activity. Incubation of callus or the inoculum employed for low density cell suspension cultures and microdroplet cell cultures with polyethylene glycol was found critical for induction of somatic embryogenesis. Somatic embryogenesis at a frequency of 1.19, 3.16 and 6.51 per 10(6) cells was achieved in the callus, low density cell suspension cultures and microdroplet cell cultures, respectively. Advantages of employing microdroplet cell cultures for high frequency production of somatic embryos and its application in genetic transformation protocols are discussed. PMID:26548080

  17. 木豆新品种‘CAF10'%A New Cajanus cajan Cultivar 'CAF10'

    Institute of Scientific and Technical Information of China (English)

    万友名; 李正红; 马宏; 刘秀贤; 谷勇; 李立; 梁宁

    2012-01-01

    ‘CAF10’是运用单株选择和集团选择育种手段,从云南元谋县地方栽培品种中选育出的木豆新品种.生育期259~264天,平均株高(264.5±32.9)cm,地径(3.86±0.65)cm,单株荚数(948.3±231.0)个,每荚粒数(4.9±0.4)粒,种子百粒质量(8.76±0.49)g,单株粒质量(203.09±70.23)g.%'CAFlO'is a new cultivar of Cajanus cajan, bred from endemic pigeonpea cultivars of Yuanmou, Yunnan province. Single plant selection and group selection were applied into the breeding course. Its growth period ranges 259 -264 days; Average plant height and ground diameter are (264.5 ±32.9) cm and (3.86 ±0.65) cm respectively; Number of pod per plant is (948. 3 ±231. 0) ; Each pod contains (4.9±0.4) beans; The 100-seed weight is ( 8. 76 ± 0. 49) g; Total seed weight per plant is up to (203. 09 ±70. 23) g.

  18. Application of pigeon pea (Cajanus cajan) stalks as raw material for xylooligosaccharides production.

    Science.gov (United States)

    Samanta, A K; Jayapal, Natasha; Kolte, A P; Senani, S; Sridhar, Manpal; Mishra, Sukriti; Prasad, C S; Suresh, K P

    2013-04-01

    Pigeon pea (Cajanus cajan) is a perennial plant widely cultivated in tropical and subtropical regions of many countries. The present studies aimed to produce xylooligosaccharides (XOS) from pigeon pea stalks in order to do value addition. The chemical analysis of stalks revealed 18.33 ± 1.40 % hemicelluloses in addition to cellulose, protein, and lignin. Sodium hydroxide coupled with steam application enabled almost 96 % recovery of original xylan, present in the pigeon pea stalks. Enzymatic hydrolysis of xylan led to production of XOS namely, xylobiose and xylotriose. Response surface model indicated a maximum yield of xylobiose (0.502 mg/ml) under the hydrolysis conditions of pH 4.91, temperature at 48.11 °C, enzyme dose at 11.01 U, and incubation time at 15.65 h. The ideal conditions for higher xylotriose yield (0.204 mg/ml) were pH 5.44, temperature at 39.29 °C, enzyme dose at 3.23 U, and incubation time at 15.26 h. The present investigation was successful in assessing the prospect of using pigeon pea stalks as a raw material for xylan extraction vis-à-vis XOS production. PMID:23456278

  19. Identification and Validation of Expressed Sequence Tags from Pigeonpea (Cajanus cajan L.) Root.

    Science.gov (United States)

    Kumar, Ravi Ranjan; Yadav, Shailesh; Joshi, Shourabh; Bhandare, Prithviraj P; Patil, Vinod Kumar; Kulkarni, Pramod B; Sonkawade, Swati; Naik, G R

    2014-01-01

    Pigeonpea (Cajanus cajan (L) Millsp.) is an important food legume crop of rain fed agriculture in the arid and semiarid tropics of the world. It has deep and extensive root system which serves a number of important physiological and metabolic functions in plant development and growth. In order to identify genes associated with pigeonpea root, ESTs were generated from the root tissues of pigeonpea (GRG-295 genotype) by normalized cDNA library. A total of 105 high quality ESTs were generated by sequencing of 250 random clones which resulted in 72 unigenes comprising 25 contigs and 47 singlets. The ESTs were assigned to 9 functional categories on the basis of their putative function. In order to validate the possible expression of transcripts, four genes, namely, S-adenosylmethionine synthetase, phosphoglycerate kinase, serine carboxypeptidase, and methionine aminopeptidase, were further analyzed by reverse transcriptase PCR. The possible role of the identified transcripts and their functions associated with root will also be a valuable resource for the functional genomics study in legume crop. PMID:24895494

  20. Increased effectiveness of competitive rhizobium strains upon inoculation of Cajanus cajan

    Energy Technology Data Exchange (ETDEWEB)

    Hernandez, B.S.; Poth, M.; Focht, D.D.

    1987-09-01

    A field study was conducted in lysimeters containing /sup 15/N-enriched soil to determine the effects of four competitive rhizobium strains upon yield parameters of pigeon peas (Cajanus cajan). The greatest differences observed were in seed yields; strain P132 effected the highest seed yield (121 +/- 20 g per plant), and the control strain (indigenous rhizobia) effected the lowest yield (43.9 +/- 8 g per plant). With the exception of seeds and pods, the dry matter weights were not different. Although there appeared to be no effect by inoculum strains on the fractional content of N derived from biological nitrogen fixation when the total plant biomass was considered, strains P132 and 401 partitioned more of the N derived from fixation into seeds and leaves than did the other strains. Because the seeds comprised the major portion of plant N, more total N and more N derived from biological nitrogen fixation (about half of total N) were found in plants inoculated with P132, whereas the smallest amount was found in the uninoculated controls. P132 was also the best competitor with respect to indigenous rhizobia and accounted for all of the nodules found on the plants in which it was inoculated.

  1. Laser Induced Chlorophyll Fluorescence Spectra of Cajanus Cajan L Plant Growing Under Cadmium Stress

    Science.gov (United States)

    Gopal, Ram; Pandey, J. K.

    2010-06-01

    Laser-induced Chlorophyll fluorescence (LICF) spectra of Cajanus cajan L leaves treated with different concentrations of Cd (0.05, 0.5 and 1 mM) are recorded at 10 and 20 days after first treatment of cadmium. LICF spectra are recorded in the region of 650-780 nm using violet diode laser (405 nm). LICF spectra of plant leaves show two maxima near 685 and 730nm. Fluorescence induction kinetics (FIK) curve are recorded at 685 and 730 nm with red diode laser (635 nm) for excitation. The fluorescence intensity ratios (FIR) F685/F730 are calculated from LICF spectra and vitality index (Rfd) are determined from FIK curve. FIR and Rfd value are good stress indicator of plant health. These parameters along with chlorophyll content are used to analyze the effect of Cd on wheat plants. The result indicates that higher concentrations of Cd hazardous for photosynthetic activity and health of Arhar plants. The lower concentration of 0.05 mM shows stimulatory response up to 10 days while after 20 days this concentration also shows inhibitory response. R. Gopal, K. B. Mishra, M. Zeeshan, S. M. Prasad, and M. M. Joshi Curr. Sci., 83, 880, 2002 K. B. Mishra and R. Gopal Int. J. Rem. Sen., 29, 157, 2008 R. Maurya, S. M. Prasad, and R. Gopal J. Photochem. Photobio. C: Photochem. Rev., 9, 29, 2008

  2. Germinated Pigeon Pea (Cajanus cajan): a novel diet for lowering oxidative stress and hyperglycemia.

    Science.gov (United States)

    Uchegbu, Nneka N; Ishiwu, Charles N

    2016-09-01

    This work studied the antioxidant activity of extract of germinated pigeon pea (Cajanus cajan) in alloxan-induced diabetic rats. Germination was carried out in a dark chamber under room temperature (28°C). The total phenolic, 1,1,diphenyl-2-picrylhy-drazyl free radical (DPPH) scavenging, the inhibition of α-amylase and α-glucosidase were done in vitro and blood glucose levels of the animal were investigated. Lipid peroxidation (LPO) and reduced glutathione (GSH) were analyzed spectrophotometrically. The total phenolic and DPPH scavenging activity increased by 30% and 63%, respectively, after germinating pigeon pea. Also after germination there was an increase in the inhibitory potential of pigeon pea extract against α-glucosidase compared with the nongerminated pigeon pea extract. There was a significant increase (P germinated pigeon pea extract gave rise to a reduced fasting blood glucose level in diabetic rats. On administration of germinated pigeon pea extract, LPO reduced drastically but there was an increase in the level of GSH. This study concluded that intake of germinated pigeon pea is a good dietary supplement for controlling hyperglycemia and LPO. PMID:27625782

  3. Pigeonpea (Cajanus cajan L.) urease immobilized on glutaraldehyde-activated chitosan beads and its analytical applications.

    Science.gov (United States)

    Kayastha, A M; Srivastava, P K

    2001-01-01

    Urease from pigeonpea (Cajanus cajan L.) was covalently linked to crab shell chitosan beads using glutaraldehyde. The optimum immobilization (64% activity) was observed at 4 degrees C, with a protein concentration of 0.24 mg/bead and 3% glutaraldehyde. The immobilized enzyme stored in 0.05 M Tris-acetate buffer, pH 7.3, at 4 degrees C had a t(1/2) of 110 d. There was practically no leaching of enzyme (chitosan-immobilized urease showed a significantly higher Michaelis constant (8.3 mM) compared to that of the soluble urease (3.0 mM). Its apparent optimum pH also shifted from 7.3 to 8.5. Immobilized urease showed an optimal temperature of 77 degrees C, compared with 47 degrees C for the soluble urease. Time-dependent kinetics of the thermal denaturation of immobilized urease was studied and found to be monophasic in nature compared to biphasic in nature for soluble enzyme. This immobilized urease was used to analyze blood urea of some of the clinical samples from the clinical pathology laboratories. The results compared favorably with those obtained by the various chemical/biochemical methods employed in the clinical pathology laboratories. A column packed with immobilized urease beads was also prepared in a syringe for the regular and continuous monitoring of serum urea concentrations.

  4. Identification of amylase inhibitor deficient mutants in pigeonpea (Cajanus cajan (L.) Millisp.).

    Science.gov (United States)

    Chougule, N P; Giri, A P; Hivrale, V K; Chhabda, P J; Kachole, M S

    2004-06-01

    We have developed and analyzed several mutant lines (M6 generation) of pigeonpea (Cajanus cajan (L.) Millsp.) for the content of defensive proteins and antinutritional factors. Inhibitors of proteinase and of amylase, lectins, and raffinose family oligosaccharides were analyzed in mature seeds of different pigeonpea accessions (untreated) and compared with mutant lines. Proteinase inhibitor profiles were similar in terms of number and intensities of activity bands but they differ marginally in the activity units in pigeonpea accessions and mutants. Pigeonpea mutants showed significant differences in amylase inhibitor profiles as well as activity units from those of pigeonpea accessions. Interestingly, two mutants (A6-5-1 and A7-3-2) were identified to have absence of amylase inhibitor isoforms. Hemagglutinating activity and raffinose family oligosaccharides content were found to be significantly higher in mutants than in accessions. It is evident from the results that proteinase inhibitors of pigeonpea are stable while amylase inhibitors, lectins, and raffinose family oligosaccharides show altered expression upon mutagen treatments. These mutants will be ideal candidates for further evaluation. PMID:15260142

  5. Production of Phaseolus vulgaris L. Genotypes with Tithonia diversifolia (Hemsl. Gray and Cajanus cajan (L. Millsp.

    Directory of Open Access Journals (Sweden)

    Donald C. L. Kass

    2013-03-01

    Full Text Available Adding mulch biomass prior to crop seeding may improve production of tropical soil. We evaluated the response of four bean (Phaseolus vulgaris L. genotypes to the addition of mulch biomass from Tithonia diversifolia (Hemsl. Gray and Cajanus cajan L. Millsp. The addition of mulch did not result in significant differences (p < 0.05 in soil characteristics when compared to a control (no mulch addition except for soil potassium (K, which was significantly greater (p < 0.05 in the T. diversifolia mulch biomass treatment. Bean yield and shoot biomass were significantly greater (p < 0.05 in the mulch biomass treatments compared to the control (no biomass added. In these treatments, Phosphorus (P-efficient bean genotypes had a significantly greater (p < 0.05 yield and shoot biomass. Bean shoot nutrient concentrations were significantly different (p < 0.05 between mulch biomass treatments and between bean genotypes (P, K and magnesium (Mg only. Phosphorus utilization and uptake efficiencies were significantly different (p < 0.05 between mulch biomass treatments and between bean genotypes. Bean root biomass was not significantly different (p < 0.05 between mulch biomass treatments, but was significantly different (p < 0.05 between bean genotypes. The number of root nodules was significantly greater (p < 0.05 in the T. diversifolia mulch biomass treatment and was significantly different between bean genotypes.

  6. Crop wild relatives of pigeonpea [Cajanus cajan (L.) Millsp.]: Distributions, ex situ conservation status, and potential genetic resources for abiotic stress tolerance

    NARCIS (Netherlands)

    Khoury, C.K.; Castaneda-Alvarez, N.P.; Achicanoy, H.A.; Sosa, C.C.; Bernau, V.; Kassa, M.T.; Norton, S.L.; Maesen, L.; Upadhyaya, H.D.; Ramirez-Villegas, J.; Jarvis, A.; Struik, P.C.

    2015-01-01

    Pigeonpea [Cajanus cajan (L.) Millsp.] is a versatile, stress-tolerant, and nutritious grain legume, possessing traits of value for enhancing the sustainability of dry sub-tropical and tropical agricultural systems. The use of crop wild relatives (CWR) in pigeonpea breeding has been successful in pr

  7. Modifications on leaf anatomy of Coffea arabica caused by shade of pigeonpea (Cajanus cajan

    Directory of Open Access Journals (Sweden)

    Heverly Morais

    2004-11-01

    Full Text Available Modifications on leaf anatomy in Coffea arabica shaded with pigeonpea (Cajanus cajan, compared to cultivation under full sun, were assessed. The leaves fully exposed to sunlight presented thicker cuticles and cellular walls, narrower epidermis cells, palisade parenchyma with longer cells, thicker lacunar parenchyma, fewer intercellular spaces and a larger stomata number. Leaves under dense shade presented a narrower cuticle and cellular wall; a mesophyll with smaller volume, but with larger intercellular spaces; and epidermis with thicker cells and a smaller stomata amount, surrounded by subsidiary cells of smaller dimensions. Plants grown under full sunlight presented higher values of net photosynthesis. The results evidenced that the species C. arabica has a wide range of phenotypic adaptation to changes in the radiation intensity.Adaptações de plantas da mesma espécie aos diferentes habitats, constituem a base da diferenciação entre folhas a pleno sol e folhas sob sombra e estão associadas a características anatômicas distintas. Para caracterizar tais mudanças em cafeeiros cultivados sob sombra de guandu (Cajanus cajan e a pleno sol, em Londrina, PR, foram realizadas avaliações de modificações ocorridas na anatomia foliar. As estruturas internas avaliadas foram: parede celular e cutícula; epiderme; mesofilo (parênquima paliçádico, parênquima lacunoso e espaços intercelulares e estômatos. Para todas as variáveis avaliadas observaram-se diferenças anatômicas entre folhas expostas ao sol e à sombra. As folhas expostas ao sol apresentaram cutículas e paredes celulares mais espessas, células da epiderme mais estreitas, parênquima paliçádico com células mais alongadas, parênquima lacunoso espesso e com poucos espaços intercelulares e maior número de estômatos. Folhas sob condições de denso sombreamento apresentam menor espessamento da cutícula e da parede celular; mesofilos com menores volumes, porém com

  8. Structural and functional characterization of proteinase inhibitors from seeds of Cajanus cajan (cv. ICP 7118).

    Science.gov (United States)

    Swathi, Marri; Lokya, Vadthya; Swaroop, Vanka; Mallikarjuna, Nalini; Kannan, Monica; Dutta-Gupta, Aparna; Padmasree, Kollipara

    2014-10-01

    Proteinase inhibitors (C11PI) from mature dry seeds of Cajanus cajan (cv. ICP 7118) were purified by chromatography which resulted in 87-fold purification and 7.9% yield. SDS-PAGE, matrix assisted laser desorption ionization time-of-flight (MALDI-TOF/TOF) mass spectrum and two-dimensional (2-D) gel electrophoresis together resolved that C11PI possessed molecular mass of 8385.682 Da and existed as isoinhibitors. However, several of these isoinhibitors exhibited self association tendency to form small oligomers. All the isoinhibitors resolved in Native-PAGE and 2-D gel electrophoresis showed inhibitory activity against bovine pancreatic trypsin and chymotrypsin as well as Achaea janata midgut trypsin-like proteases (AjPs), a devastating pest of castor plant. Partial sequences of isoinhibitor (pI 6.0) obtained from MALDI-TOF/TOF analysis and N-terminal sequencing showed 100% homology to Bowman-Birk Inhibitors (BBIs) of leguminous plants. C11PI showed non-competitive inhibition against trypsin and chymotrypsin. A marginal loss (<15%) in C11PI activity against trypsin at 80 (°)C and basic pH (12.0) was associated with concurrent changes in its far-UV CD spectra. Further, in vitro assays demonstrated that C11PI possessed significant inhibitory potential (IC50 of 78 ng) against AjPs. On the other hand, in vivo leaf coating assays demonstrated that C11PI caused significant mortality rate with concomitant reduction in body weight of both larvae and pupae, prolonged the duration of transition from larva to pupa along with formation of abnormal larval-pupal and pupal-adult intermediates. Being smaller peptides, it is possible to express C11PI in castor to protect them against its devastating pest A. janata. PMID:25093261

  9. Antioxidant activities of extracts and main components of Pigeonpea [Cajanus cajan (L.) Millsp.] leaves.

    Science.gov (United States)

    Wu, Nan; Fu, Kuang; Fu, Yu-Jie; Zu, Yuan-Gang; Chang, Fang-Rong; Chen, Yung-Husan; Liu, Xiao-Lei; Kong, Yu; Liu, Wei; Gu, Cheng-Bo

    2009-01-01

    Antioxidant activities of the aqueous and ethanol extracts of pigeonpea [Cajanus cajan (L.) Millsp.] leaves, as well as petroleum ether, ethyl acetate, n-butanol and water fractions and the four main compounds separated from the ethanol extract, i.e. cajaninstilbene acid (3-hydroxy-4-prenylmethoxystilbene-2-carboxylic acid), pinostrobin, vitexin and orientin, were examined by a DPPH radical-scavenging assay and a beta-carotene-linoleic acid test. In the DPPH system, the antioxidant activity of the ethanol extracts was superior to that of the aqueous extracts, with IC(50) values were 242.01 and 404.91 microg/mL, respectively. Among the four fractions, the ethyl acetate one showed the highest scavenging activity, with an IC(50) value of 194.98 microg/mL. Cajaninstilbene acid (302.12 microg/mL) and orientin (316.21 microg/mL) showed more efficient radical-scavenging abilities than pinostrobin and vitexin. In the beta-carotene-linoleic acid test, the inhibition ratio (%) of the ethyl acetate fraction (94.13%+/-3.41%) was found to be the highest, being almost equal to the inhibition capacity of the positive control BHT (93.89%+/-1.45%) at 4 mg/mL. Pinostrobin (>500 microg/mL) and vitexin (>500 microg/mL) showed insignificant antioxidant activities compared with cajaninstilbene (321.53 microg/mL) and orientin (444.61 microg/mL). In general, the ethyl acetate fraction of the ethanol extract showed greater activity than the main compounds in both systems, such results might be attributed to the synergistic effects of the components. The antioxidant activities of all the tested samples were concentration-dependent. Based on the results obtained, we can conclude that the pigeonpea leaf extracts may be valuable natural antioxidant sources and are potentially applicable in both medicine and the healthy food industry. PMID:19305357

  10. Antioxidant Activities of Extracts and Main Components of Pigeonpea [Cajanus cajan (L. Millsp.] Leaves

    Directory of Open Access Journals (Sweden)

    Xiao-Lei Liu

    2009-03-01

    Full Text Available Antioxidant activities of the aqueous and ethanol extracts of pigeonpea [Cajanus cajan (L. Millsp.] leaves, as well as petroleum ether, ethyl acetate, n-butanol and water fractions and the four main compounds separated from the ethanol extract, i.e. cajaninstilbene acid (3-hydroxy-4-prenylmethoxystilbene-2-carboxylic acid, pinostrobin, vitexin and orientin, were examined by a DPPH radical-scavenging assay and a β-carotene-linoleic acid test. In the DPPH system, the antioxidant activity of the ethanol extracts was superior to that of the aqueous extracts, with IC50 values were 242.01 and 404.91 µg/mL, respectively. Among the four fractions, the ethyl acetate one showed the highest scavenging activity, with an IC50 value of 194.98 µg/mL. Cajaninstilbene acid (302.12 µg/mL and orientin (316.21 µg/mL showed more efficient radical-scavenging abilities than pinostrobin and vitexin. In the β-carotene-linoleic acid test, the inhibition ratio (% of the ethyl acetate fraction (94.13%±3.41% was found to be the highest, being almost equal to the inhibition capacity of the positive control BHT (93.89%±1.45% at 4 mg/mL. Pinostrobin (>500 µg/mL and vitexin (>500 µg/mL showed insignificant antioxidant activities compared with cajaninstilbene (321.53 µg/mL and orientin (444.61 µg/mL. In general, the ethyl acetate fraction of the ethanol extract showed greater activity than the main compounds in both systems, such results might be attributed to the synergistic effects of the components. The antioxidant activities of all the tested samples were concentration-dependent. Based on the results obtained, we can conclude that the pigeonpea leaf extracts may be valuable natural antioxidant sources and are potentially applicable in both medicine and the healthy food industry.

  11. Identification and validation of selected universal stress protein domain containing drought-responsive genes in pigeonpea (Cajanus cajan L.

    Directory of Open Access Journals (Sweden)

    Pallavi eSinha

    2016-01-01

    Full Text Available Pigeonpea is a resilient crop, which is relatively more drought tolerant than many other legume crops. To understand the molecular mechanisms of this unique feature of pigeonpea, 51 genes were selected using the Hidden Markov Models those codes for proteins having close similarity to universal stress protein domain. Validation of these genes was conducted on three pigeonpea genotypes (ICPL 151, ICPL 8755 and ICPL 227 having different levels of drought tolerance. Gene expression analysis using qRT-PCR revealed 6, 8 and 18 genes to be ≥2 fold differentially expressed in ICPL 151, ICPL 8755 and ICPL 227, respectively. A total of 10 differentially expressed genes showed ≥2 fold up-regulation in the more drought tolerant genotype. Of these, four genes each encoded proteins for plant U-box and universal stress protein A- (uspA like, while one gene encoded for cation/H(+ antiporter protein and one uncharacterized protein. Genes C.cajan_29830 and C.cajan_33874 belonging to uspA, were found significantly expressed in all the three genotypes with ≥2 fold expression variations. Expression profiling of these two genes on the four other legume crops revealed their specific role in pigeonpea. Therefore, these genes seem to be promising candidates for conferring drought tolerance specifically to pigeonpea.

  12. Identification and Validation of Selected Universal Stress Protein Domain Containing Drought-Responsive Genes in Pigeonpea (Cajanus cajan L.).

    Science.gov (United States)

    Sinha, Pallavi; Pazhamala, Lekha T; Singh, Vikas K; Saxena, Rachit K; Krishnamurthy, L; Azam, Sarwar; Khan, Aamir W; Varshney, Rajeev K

    2015-01-01

    Pigeonpea is a resilient crop, which is relatively more drought tolerant than many other legume crops. To understand the molecular mechanisms of this unique feature of pigeonpea, 51 genes were selected using the Hidden Markov Models (HMM) those codes for proteins having close similarity to universal stress protein domain. Validation of these genes was conducted on three pigeonpea genotypes (ICPL 151, ICPL 8755, and ICPL 227) having different levels of drought tolerance. Gene expression analysis using qRT-PCR revealed 6, 8, and 18 genes to be ≥2-fold differentially expressed in ICPL 151, ICPL 8755, and ICPL 227, respectively. A total of 10 differentially expressed genes showed ≥2-fold up-regulation in the more drought tolerant genotype, which encoded four different classes of proteins. These include plant U-box protein (four genes), universal stress protein A-like protein (four genes), cation/H(+) antiporter protein (one gene) and an uncharacterized protein (one gene). Genes C.cajan_29830 and C.cajan_33874 belonging to uspA, were found significantly expressed in all the three genotypes with ≥2-fold expression variations. Expression profiling of these two genes on the four other legume crops revealed their specific role in pigeonpea. Therefore, these genes seem to be promising candidates for conferring drought tolerance specifically to pigeonpea. PMID:26779199

  13. Next-generation sequencing for identification of candidate genes for Fusarium wilt and sterility mosaic disease in pigeonpea (Cajanus cajan).

    Science.gov (United States)

    Singh, Vikas K; Khan, Aamir W; Saxena, Rachit K; Kumar, Vinay; Kale, Sandip M; Sinha, Pallavi; Chitikineni, Annapurna; Pazhamala, Lekha T; Garg, Vanika; Sharma, Mamta; Sameer Kumar, Chanda Venkata; Parupalli, Swathi; Vechalapu, Suryanarayana; Patil, Suyash; Muniswamy, Sonnappa; Ghanta, Anuradha; Yamini, Kalinati Narasimhan; Dharmaraj, Pallavi Subbanna; Varshney, Rajeev K

    2016-05-01

    To map resistance genes for Fusarium wilt (FW) and sterility mosaic disease (SMD) in pigeonpea, sequencing-based bulked segregant analysis (Seq-BSA) was used. Resistant (R) and susceptible (S) bulks from the extreme recombinant inbred lines of ICPL 20096 × ICPL 332 were sequenced. Subsequently, SNP index was calculated between R- and S-bulks with the help of draft genome sequence and reference-guided assembly of ICPL 20096 (resistant parent). Seq-BSA has provided seven candidate SNPs for FW and SMD resistance in pigeonpea. In parallel, four additional genotypes were re-sequenced and their combined analysis with R- and S-bulks has provided a total of 8362 nonsynonymous (ns) SNPs. Of 8362 nsSNPs, 60 were found within the 2-Mb flanking regions of seven candidate SNPs identified through Seq-BSA. Haplotype analysis narrowed down to eight nsSNPs in seven genes. These eight nsSNPs were further validated by re-sequencing 11 genotypes that are resistant and susceptible to FW and SMD. This analysis revealed association of four candidate nsSNPs in four genes with FW resistance and four candidate nsSNPs in three genes with SMD resistance. Further, In silico protein analysis and expression profiling identified two most promising candidate genes namely C.cajan_01839 for SMD resistance and C.cajan_03203 for FW resistance. Identified candidate genomic regions/SNPs will be useful for genomics-assisted breeding in pigeonpea. PMID:26397045

  14. Anti-oxidative protection against iron overload-induced liver damage in mice by Cajanus cajan (L.) Millsp. leaf extract.

    Science.gov (United States)

    Sarkar, Rhitajit; Hazra, Bibhabasu; Mandal, Nripendranath

    2013-02-01

    In view of the contribution of iron deposition in the oxidative pathologic process of liver disease, the potential of 70% methanolic extract of C. cajan leaf (CLME) towards antioxidative protection against iron-overload-induced liver damage in mice has been investigated. DPPH radical scavenging and protection of Fenton reaction induced DNA damage was conducted in vitro. Post oral administration of CLME to iron overloaded mice, the levels of antioxidant and serum enzymes, hepatic iron, serum ferritin, lipid peroxidation, and protein carbonyl and hydroxyproline contents were measured, in comparison to deferasirox treated mice. Oral treatment of the plant extract effectively lowered the elevated levels of liver iron, lipid peroxidation, protein carbonyl and hydroxyproline. There was notable increment in the dropped levels of hepatic antioxidants. The dosage of the plant extract not only made the levels of serum enzymes approach normal value, but also counteracted the overwhelmed serum ferritin level. The in vitro studies indicated potential antioxidant activity of CLME. The histopathological observations also substantiated the ameliorative function of the plant extract. Accordingly, it is suggested that Cajanus cajan leaf can be a useful herbal remedy to suppress oxidative damage caused by iron overload. PMID:23923610

  15. Therapeutic and protective effects of Caesalpinia gilliesii and Cajanus cajan proteins against acetaminophen overdose-induced renal damage.

    Science.gov (United States)

    Aly, Hanan F; Rizk, Maha Z; Abo-Elmatty, Dina M; Desoky, M M; Ibrahim, N A; Younis, Eman A

    2016-04-01

    The present work aims to evaluate the protective and ameliorative effects of two plant-derived proteins obtained from the seeds of Cajanus cajan and Caesalpinia gilliesii(Leguminosae) against the toxic effects of acetaminophen in kidney after chronic dose through determination of certain biochemical markers including total urea, creatinine, and kidney marker enzyme, that is, glyceraldehyde-3-phosphate dehydrogenase (GAPDH). In addition histopathological examination of intoxicated and treated kidney with both proteins was performed. The present results show a significant increase in serum total urea and creatinine, while significant decrease in GAPDH. Improvement in all biochemical parameters studied was demonstrated, which was documented by the amelioration signs in rats kidney architecture. Thus, both plant protein extracts can counteract the nephrotoxic process, minimize damage to the kidney, delay disease progression, and reduce its complications. PMID:24280655

  16. Caractérisation du savoir paysan sur les insectes nuisibles du pois d'Angole (Cajanus Cajan (L. Millsp. dans le Nord de l'Ouganda

    Directory of Open Access Journals (Sweden)

    Velay F.

    2001-01-01

    Full Text Available Characterization of indigenous traditional knowledge on insect pests of pigeonpea (Cajanus cajan (L. Millsp. in Northern Uganda. In order to assess the perception of the main insect pests of pigeonpea (Cajanus cajan (L. Millsp. by small farmers and to inventoriate methods traditionally used to control these insects, a survey was conducted in 41 households of Northern Uganda (Lira and Apac districts. Virtually all small farmers (95/ considered blister beetle (Mylabris sp., which is the most conspicious insect damaging pigeonpea at the flowering stage, as the main pest of the crop. This is conflicting with field observations made earlier in Northern Uganda which pointed out pod borers (Helicoverpa armigera Huebner and Maruca vitrata Geyer and pod sucking bugs (mainly Clavigralla sp. as the most damaging pests for pigeonpea production. A new pest of pigeonpea, Luprops baldius Mueller, was identified during this survey. Its adults and larvae are damaging to mature pods in case of late harvest.

  17. Introgression of Helicoverpa armigera Resistance from Cajanus acutifolius-a Wild Relative from Secondary Gene Pool of Pigeon Pea (Cajanus cajan)

    OpenAIRE

    Deepak R. Jadhav; Nalini Mallikarjuna; Sharma, Hari C; Kulbhushan B. Saxena

    2012-01-01

    The aim of the study was to introgress Helicoverpa armigera resistance from wild relative Cajanus acutifolius into pigeonpea, (Cajanus cajan L.), an important grain legume in South Asia, East Africa and the West Indies. Pigeonpea grain yields on farmer’s fields are quite low, largely because of damage by insect pests, of which legume pod borer Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) is the important pest worldwide. Pod borer has developed high levels of resistance to chemical i...

  18. A New Cajanus cajan Cultivar ‘ CAF11'%木豆新品种‘CAF11’

    Institute of Scientific and Technical Information of China (English)

    马宏; 李正红; 刘秀贤; 万友名; 谷勇; 李立; 梁宁

    2012-01-01

    ‘CAF11’是运用单株选择和集团选择育种手段,从云南墨江县地方栽培品种中选育出的木豆新品种.生育期260 ~266天,平均株高(238.1 ±28.0) cm,地径(3.90±0.75) cm,单株荚数(1 145.0±251.8)个,每荚粒数(4.9±0.3)粒,种子百粒质量(8.76±0.49)g,单株粒质量(230.59 ±73.38)g.%'CAF11 'is a new cultivar of Cajanus cajan, bred from endemic pigeonpea cultivars of Yuanjiang, Yunnan province by single plant selection and group selection. Its growth period ranges 260 -266 days; Average plant height and ground diameter are (238. 1 ±28. 0) cm and (3. 90 ±0. 75) cm, respectively; Number of pod per plant is ( 1 145. 0 ± 251. 8 ); Each pod contains (4. 9 ± 0. 3 ) beans; 100-seed weight is ( 8. 76 ± 0. 49) g; Total seed weight per plant is up to (230.59 ±73.38) g.

  19. 木豆新品种‘CAF9'%A New Cajanus cajan Cultivar ‘ CAF9'

    Institute of Scientific and Technical Information of China (English)

    刘秀贤; 李正红; 马宏; 万友名; 谷勇; 李立; 梁宁

    2012-01-01

    ‘CAF9’是运用单株选择和集团选择育种手段,从云南元江县地方栽培品种中选育出的木豆新品种.生育期263 ~ 270天,平均株高(267.2±35.2) cm,地径(3.97±0.53) cm,单株荚数(600.3±207.6)个,每荚粒数(4.9±0.3)粒,种子百粒质量(7.86±0.43)g,单株粒质量(132.45±49.50)g.%CAF9 ' is a new cultivar of pigeonpea (Cajanus cajan), bred from endemic pigeonpea cultivars of Yuanjiang, Yunnan province. Individual plant selection and group selection were applied in the breeding course. Its growth period ranges 263 - 270 days. Average plant height and ground diameter are ( 267. 2 ± 35. 2) cm and ( 3. 97 ± 0.53) cm respectively. Number of pod per plant ia (600.3 ±207.6) and each pod contains (4. 9 ±0.3) beans; The 100-seed weight is (7. 86 ±0. 43) g and total seed weight per plant is up to ( 132. 45 ±49. 50) g.

  20. A New Cajanus cajan Cultivar ' CAF8'%木豆新品种‘CAF8’

    Institute of Scientific and Technical Information of China (English)

    马宏; 李正红; 刘秀贤; 万友名; 谷勇; 李立; 梁宁

    2012-01-01

    The ' CAF8 ' is a new cultivar of Cajanus cajan, bred from endemic pigeonpea cultivars of Yuanjiang, Yunnan Province. The single plant selection and group selection were applied into the breeding course. Its growth period ranges 257-265 days; The average plant height and ground diameter are (231.5 ±32.7) cm and (3.74 ±0.76) cm respectively; The number of pod per plant is (794.8 ±272.6); Each pod contains (4.5 ±0.4) beans; the 100-seed weight is (7. 85 ±0. 51) g; The total seed weight per plant is up to (81. 17 ±23. 40) g.%‘CAF8’是运用单株选择和集团选择育种手段,从云南元江县地方栽培品种中选育出的木豆新品种.生育期257~265天,平均株高(231.5±32.7) cm,地径(3.74±0.76) cm,单株荚致(794.8±272.6)个,每荚粒数(4.5±0.4)粒,种子百粒质量(7.85±0.51)g,单株粒质量(81.17±23.40)g.

  1. Constituents of Cajanus cajan (L.) Millsp., Moringa oleifera Lam., Heliotropium indicum L. and Bidens pilosa L. from Nigeria.

    Science.gov (United States)

    Ogunbinu, Akinola O; Flamini, Guido; Cioni, Pier L; Adebayo, Muritala A; Ogunwande, Isiaka A

    2009-04-01

    The essential oils of four plant species from Nigeria have been extracted by hydrodistillation and analyzed by GC and GC-MS. The oils of Cajanus cajan were comprised of sesquiterpenes (92.5%, 81.2% and 94.3% respectively in the leaves, stem and seeds). The major compounds identified were alpha-himachalene (9.0-11.5%), beta-himachalene (8.0-11.0%), gamma-himachalene (6.9-8.1%), alpha-humulene (7.1-8.7%) and alpha-copaene (4.5-5.6%). However, monoterpenoid compounds (81.8%) dominated the oil of Moringa oleifera with an abundance of alpha-phellandrene (25.2%) and p-cymene (24.9%). On the other hand, aldehydes (52.8%) occurred in the highest amount in Heliotropium indicum, represented by phenylacetaldehyde (22.2%), (E)-2-nonenal (8.3%) and (E, Z)-2-nonadienal (6.1%), with a significant quantity of hexahydrofarnesylacetone (8.4%). The leaf and stem oils of Bidens pilosa were dominated by sesquiterpenes (82.3% and 59.3%, respectively). The main compounds in the leaf oil were caryophyllene oxide (37.0%), beta-caryophyllene (10.5%) and humulene oxide (6.0%), while the stem oils had an abundance of hexahydrofarnesyl acetone (13.4%), delta-cadinene (12.0%) and caryophyllene oxide (11.0%). The observed chemical patterns differ considerably from previous investigations. PMID:19476009

  2. PATTERN OF PLANT REGENERATION FROM SHOOT TIP EXPLANTS OF PIGEONPEA (CAJANUS CAJAN L MILLLSP VAR LRG-41

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    T. Raghavendra

    2014-02-01

    Full Text Available An efficient direct shoot bud differentiation and multiple shoot induction from shoot tip explants of pigeon pea (Cajanus cajan L. has been achieved. The frequency of shoot bud regeneration was influenced by the type of explants, genotype and concentrations of cytokinin. Explants viz. shoot tip isolated from 10 day old seedlings showed better explants response Explants were cultured on Murashige and skoog (MS medium augmented with different concentrations of BAP and NAA. Among the various concentrations tested, 2.0mg/l BAP (Benzyl amino purine and 0.1 mg/l Napthalene acetic acid (NAA were found to be the best for maximum shoot bud differentiation. Percentage, as well as the number of shoots per explant showing differentiation of shoot buds was higher on MS media supplement with BAP and optimal BAP concentration for shoot regeneration was 2mg/l. The elongated shoots were successfully rooted on MS medium containing different concentrations of auxins. Among them indole buteric acid (IBA at 1.0mg/l induced maximum frequency of rooting. Regenerated plants were successfully established in soil where 91% of them have been developed into morphologically normal and fertile plants. This method can thus be advantageously applied in the production of transgenic pigeon pea plants.

  3. PATTERN OF PLANT REGENERATION FROM SHOOT TIP EXPLANTS OF PIGEONPEA (CAJANUS CAJAN L MILLLSP VAR LRG-41

    Directory of Open Access Journals (Sweden)

    T. Raghavendra

    2014-03-01

    Full Text Available An efficient direct shoot bud differentiation and multiple shoot induction from shoot tip explants of pigeon pea (Cajanus cajan L. has been achieved. The frequency of shoot bud regeneration was influenced by the type of explants, genotype and concentrations of cytokinin. Explants viz. shoot tip isolated from 10 day old seedlings showed better explants response Explants were cultured on Murashige and skoog (MS medium augmented with different concentrations of BAP and NAA. Among the various concentrations tested, 2.0mg/l BAP (Benzyl amino purine and 0.1 mg/l Napthalene acetic acid (NAA were found to be the best for maximum shoot bud differentiation. Percentage, as well as the number of shoots per explant showing differentiation of shoot buds was higher on MS media supplement with BAP and optimal BAP concentration for shoot regeneration was 2mg/l. The elongated shoots were successfully rooted on MS medium containing different concentrations of auxins. Among them indole buteric acid (IBA at 1.0mg/l induced maximum frequency of rooting. Regenerated plants were successfully established in soil where 91% of them have been developed into morphologically normal and fertile plants. This method can thus be advantageously applied in the production of transgenic pigeon pea plants.

  4. Evaluation and validation of housekeeping genes as reference for gene expression studies in pigeonpea (Cajanus cajan) under drought stress conditions.

    Science.gov (United States)

    Sinha, Pallavi; Singh, Vikas K; Suryanarayana, V; Krishnamurthy, L; Saxena, Rachit K; Varshney, Rajeev K

    2015-01-01

    Gene expression analysis using quantitative real-time PCR (qRT-PCR) is a very sensitive technique and its sensitivity depends on the stable performance of reference gene(s) used in the study. A number of housekeeping genes have been used in various expression studies in many crops however, their expression were found to be inconsistent under different stress conditions. As a result, species specific housekeeping genes have been recommended for different expression studies in several crop species. However, such specific housekeeping genes have not been reported in the case of pigeonpea (Cajanus cajan) despite the fact that genome sequence has become available for the crop. To identify the stable housekeeping genes in pigeonpea for expression analysis under drought stress conditions, the relative expression variations of 10 commonly used housekeeping genes (EF1α, UBQ10, GAPDH, 18SrRNA, 25SrRNA, TUB6, ACT1, IF4α, UBC and HSP90) were studied on root, stem and leaves tissues of Asha (ICPL 87119). Three statistical algorithms geNorm, NormFinder and BestKeeper were used to define the stability of candidate genes. geNorm analysis identified IF4α and TUB6 as the most stable housekeeping genes however, NormFinder analysis determined IF4α and HSP90 as the most stable housekeeping genes under drought stress conditions. Subsequently validation of the identified candidate genes was undertaken in qRT-PCR based gene expression analysis of uspA gene which plays an important role for drought stress conditions in pigeonpea. The relative quantification of the uspA gene varied according to the internal controls (stable and least stable genes), thus highlighting the importance of the choice of as well as validation of internal controls in such experiments. The identified stable and validated housekeeping genes will facilitate gene expression studies in pigeonpea especially under drought stress conditions. PMID:25849964

  5. An Elite Cajanus cajan Variety ‘ICP7035'%木豆良种‘ICP7035'

    Institute of Scientific and Technical Information of China (English)

    马宏; 李正红; 刘秀贤; 万友名; 谷勇

    2013-01-01

    An elite variety of Cajanus cajan' ICP7035 ' , bred by International Crops Research Institute for the Semi-Arid Tropics, was introduced to China in 1998. Its growth period ranged 223 -236 days; Average height and ground diameter of two-year-old plants were ( 247. 1 ± 23. 9 ) cm and (2. 67 ± 0. 35) cm respectively; ; Number of the first order branch was (22. 0 ±2. 5) and average number of pod per plant was (382. 5 ±65. 5) ; 100-seed weight was (21. 72 ±0. 39)g; Total seed weight per plant was up to (273. 23 ±51. 58) g. It had characteristics of higher yield, bigger legumes and seeds, better taste of fresh seeds, comparing with the control varieties.%‘ICP7035’为印度国际半干旱热带作物研究所(ICRISAT)选育的纯系品种,于1998年引进中国.生育期223 ~236天,2年生植株平均株高(247.1±23.9) cm,地径(2.67-0.35)cm,一级分枝数(22.0±2.5)枝,单株荚数(382.5±65.5)个,种子百粒质量(21.72±0.39)g,单株粒质量(273.23±51.58)g;与对照品种比较,具产量高、豆荚及豆粒大、鲜籽粒口感好等优点.

  6. Introgression of Helicoverpa armigera Resistance from Cajanus acutifolius-a Wild Relative from Secondary Gene Pool of Pigeon Pea (Cajanus cajan

    Directory of Open Access Journals (Sweden)

    Deepak R. Jadhav

    2012-07-01

    Full Text Available The aim of the study was to introgress Helicoverpa armigera resistance from wild relative Cajanus acutifolius into pigeonpea, (Cajanus cajan L., an important grain legume in South Asia, East Africa and the West Indies. Pigeonpea grain yields on farmer’s fields are quite low, largely because of damage by insect pests, of which legume pod borer Helicoverpa armigera (Hübner (Lepidoptera: Noctuidae is the important pest worldwide. Pod borer has developed high levels of resistance to chemical insecticides. Currently, there are no cultivars of pigeonpea with high levels of resistance to H. armigera. Therefore, there is a need to identify and introgress resistance genes from the wild relatives of this crop. Wild relative of pigeonpea, Cajanus acutifolius (ICPW 15613 and the interspecific derivatives C. acutifolius x C. cajan have shown resistance to H. armigera. The results showed that all the test lines and C. acutifolius had high levels of flavonoids such as chlorogenic acid, quercetin and rutin in the flowers and buds, which may have resulted in less damage due to H. armigera larvae. Most of the test lines had more than 15.00 g of seed weight (100 seed weight and beige seed color. These lines can be used for pigeonpea improvement for resistance to H. armigera.

  7. 边坡先锋植物木豆的抗旱性%Drought Resistance Research for Slope Pioneer Plant Cajanus cajan

    Institute of Scientific and Technical Information of China (English)

    许翩翩; 王建柱

    2016-01-01

    Objective] To explore the effects of drought stress on photosynthesis,physiological and biochemical indices of Cajanus cajan,and to provide scientific references for species screening and community reconstruction in ecological restoration of hydropower slope.[ Method] Pot wa-ter control method was used for drought stress of C.cajan.Changes of net photosynthetic rate (Pn),stomatal conductance (Gs),transpiration rate (Tr),intercellular CO2 concentration (Ci),water use efficiency (Pn/Tr),malondialdehyde (MDA),proline (Pro) and soluble glucose con-tent were detected in C.cajan.[Result] C.cajan seedlings were greatly affected by the moisture change.Under drought stress,the Pn,Gs,Tr, Ci and Pn/Tr all significantly decreased,showing a negative photosynthetic response to the drought stress.MDA,Pro and soluble glucose content significantly increased,so as to effectively respond to drought stress.[ Conclusion] C.cajan has strong draught tolerance,can adapt to the ecolog-ical environment of dry-heat area.It is the optimal shrub to control degraded mountain land.%[目的]探究干旱胁迫对木豆光合及生理生化指标的影响,为水电边坡生态修复中的物种筛选、植被群落的重建提供参考。[方法]采用盆栽控水法进行干旱胁迫,测定木豆叶片净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、胞间CO2浓度(Ci)、水分利用效率(Pn/Tr)、丙二醛(MDA)含量、脯氨酸(Pro)含量和可溶性糖含量8个抗旱指标的变化。[结果]木豆幼苗受水分变化的影响显著,在干旱胁迫下Pn、Gs、Tr、Ci、Pn/Tr等光合指标均显著下降,表现出不利的负向生理生态学响应;木豆叶片MDA、Pro和可溶性糖含量均显著增加以有效应对干旱胁迫。[结论]木豆的抗旱能力较强,适应于干热地区的生态环境,是水电边坡治理与开发的优选灌木。

  8. Determination of longistylin A and longistylin C in Cajanus cajan%树豆菧类化合物longistylin A和longistylin C的检测

    Institute of Scientific and Technical Information of China (English)

    王达武; 肖敏勋; 李宜融; 沈小玲; 卢元媛; 刘抗伦; 李正红; 胡英杰

    2011-01-01

    Objective: To establish quality control criteria for medicinal herb Cajanus cajan based on the determination of longistylin A and longistylin C, two bioactive and specific stilbenes of the plant Method: Longistylin A and longistylin C were obtained from the leaves of C. cajan by silica gel column chromatography and identified as marker compounds of this plant by spectroscop-ic analysis. A RP-HPLC method was established to determine the two compounds. Result; Longistylin A and longistylin C were well separated on a Thermo BDS Hypersil C18 column (4. 6 mm x250 mm, S μm) with a mobile phase methanol-water (8:2) , and showed good linearity in the range of 0. 002 88-0.057 6 μg and 0. 011 2-0. 224 μg, respectively. The average recoveries were 98. 9% and 97. 2% with RSD of 2.4% and 2. 2% for these two compounds, respectively. Conclusion: The established analysis method is simple and accurate, whicn can be used for quality control of C. cajan.%目的:建立以药物活性和专属性菧类成分longistylin A与longistylin C为评价指标的树豆(木豆)药材质量控制方法.方法:采用硅胶柱色谱分离纯化有关成分,光谱技术鉴定化学结构;反相高效液相色谱法测定指标成分的含量.结果:从树豆叶分离鉴定了2种指标成分longistylin A和longistylin C;采用Thermo BDS Hypersil C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-水(80:20)为流动相可使指标成分获得良好分离;longistylin A和longistylin C分别在0.002 88~0.057 6μg和0.011 2~0.224 μg呈良好的线性关系,平均回收率分别为98.9%,97.2%,RSD分别为2.4%,2.2%.结论:建立了以树豆叶中药物活性菧类成分为指标的药物分析方法,可用于树豆(木豆)药材的质量控制.

  9. A Halogen-Containing Stilbene Derivative from the Leaves of Cajanus cajan that Induces Osteogenic Differentiation of Human Mesenchymal Stem Cells.

    Science.gov (United States)

    Cai, Jia-Zhong; Tang, Rong; Ye, Gui-Fu; Qiu, Sheng-Xiang; Zhang, Nen-Ling; Hu, Ying-Jie; Shen, Xiao-Ling

    2015-01-01

    A new natural halogen-containing stilbene derivative was isolated from the leaves of Cajanus cajan (L.) Millsp. and identified as 3-O-(3-chloro-2-hydroxyl-propanyl)-longistylin A by comprehensive spectroscopic and chemical analysis, and named cajanstilbene H (1). It is the first halogen-containing stilbene derivative found from plants. In human mesenchymal stem cells (hMSC) from bone marrow, 1 did not promote cell proliferation, but distinctly enhanced osteogenic differentiation of hMSC in time- and dose-dependent manners. In six human cancer cell lines, 1 showed a moderate inhibitory effect on cell proliferation, with IC50 values of 21.42-25.85 μmol·L(-1). PMID:26111172

  10. A Halogen-Containing Stilbene Derivative from the Leaves of Cajanus cajan that Induces Osteogenic Differentiation of Human Mesenchymal Stem Cells

    Directory of Open Access Journals (Sweden)

    Jia-Zhong Cai

    2015-06-01

    Full Text Available A new natural halogen-containing stilbene derivative was isolated from the leaves of Cajanus cajan (L. Millsp. and identified as 3-O-(3-chloro-2-hydroxyl-propanyl-longistylin A by comprehensive spectroscopic and chemical analysis, and named cajanstilbene H (1. It is the first halogen-containing stilbene derivative found from plants. In human mesenchymal stem cells (hMSC from bone marrow, 1 did not promote cell proliferation, but distinctly enhanced osteogenic differentiation of hMSC in time- and dose-dependent manners. In six human cancer cell lines, 1 showed a moderate inhibitory effect on cell proliferation, with IC50 values of 21.42–25.85 μmol·L−1.

  11. Effectiveness of native and exotic arbuscular mycorrhizal fungi on nutrient uptake and ion homeostasis in salt-stressed Cajanus cajan L. (Millsp.) genotypes.

    Science.gov (United States)

    Garg, Neera; Pandey, Rekha

    2015-04-01

    Soil salinity is an increasing problem worldwide, restricting plant growth and production. Research findings show that arbuscular mycorrhizal (AM) fungi have the potential to reduce negative effects of salinity. However, plant growth responses to AM fungi vary as a result of genetic variation in mycorrhizal colonization and plant growth responsiveness. Thus, profitable use of AM requires selection of a suitable combination of host plant and fungal partner. A greenhouse experiment was conducted to compare effectiveness of a native AM fungal inoculum sourced from saline soil and two single exotic isolates, Funneliformis mossseae and Rhizophagus irregularis (single or dual mix), on Cajanus cajan (L.) Millsp. genotypes (Paras and Pusa 2002) under salt stress (0-100 mM NaCl). While salinity reduced plant biomass and disturbed ionic status in both genotypes, Pusa 2002 was more salt tolerant and ensured higher AM fungal colonization, plant biomass and nutrient content with favourable ion status under salinity. Although all AM fungi reduced negative effects of salt stress, R. irregularis (alone or in combination with F. mosseae) displayed highest efficiency under salinity, resulting in highest biomass, yield, nutrient uptake and improved membrane stability with favourable K(+)/Na(+) and Ca(2+)/Na(+) ratios in the host plant. Higher effectiveness of R. irregularis correlated with higher root colonization, indicating that the symbiosis formed by R. irregularis had more stable viability and efficiency under salt stress. These findings enhance understanding of the functional diversity of AM fungi in ameliorating plant salt stress tolerance and suggest the potential use of R. irregularis for increasing Cajanus cajan productivity in saline soils. PMID:25155616

  12. Microwave-assisted extraction of flavonoids from Cajanus cajan leaves%木豆叶中黄酮微波提取工艺研究

    Institute of Scientific and Technical Information of China (English)

    金时; 杨梅; 孔羽; 姚晓慧; 魏作富; 祖元刚; 付玉杰

    2011-01-01

    Objective To select the proper method for extracting flavonoids from Cajanus cajan leaves and optimize the extraction parameters. Methods Taking the extracting rate of orientoside, luteolin, and total flavonoids from C. Cajan leaves and anti-oxidative activity as indexes, microwave-assisted method was compared with heat reflux extraction, ultrasound-assisted extraction, and maceration extraction. Central composite design was used for optimization of parameters affecting the extraction of orientoside and luteolin using microwave-assisted method. Results The results showed that microwave-assisted extraction was the best method and the optimal conditions were: 50 mesh particle size, 80% ethanol aqueous, 1 : 20 material/ethanol aqueous, temperature 60 ℃, 4 times cycle, 10 min extraction time, 500 W extraction power. The extraction yields of orientoside and luteolin were (4.42 ±0.01) and (0.10 ± 0.01) mg/g from dry C. Cajan leaves, respectively. Conclusion The microwave-assisted method is feasible with less time, higher extraction yield, and better anti-oxidative activity.%目的 选取适合提取木豆叶中黄酮的方法,并优化提取工艺参数.方法 以木豆叶中荭草苷、木犀草素和总黄酮的提取率及提取物抗氧化活性为指标,将微波辅助提取与热回流、超声波及浸泡提取方法相比较,并采用中心组合设计对影响荭草苷和木犀草素提取效果的微波辅助提取工艺参数进行了优化.结果 通过比较确定微波辅助提取法较为适合木豆叶中黄酮的提取;优化得到荭草苷和木犀草素最佳微波提取工艺参数为:木豆叶粒径为50目,乙醇体积分数为80%,料液比1∶20,提取温度60℃,提取次数4次,提取时间10 min,微波功率500 W;荭草苷和木犀草素的提取率分别为(4.42±0.01)、(0.10±0.01)mg/g.结论 采用微波辅助提取木豆叶中的黄酮具有提取时间短、提取率高、产物抗氧化效果好的特点,优化的工艺参数具

  13. Evaluación de tres niveles de reemplazo del concentrado comercial por guandul (Cajanus cajan L. en conejos de ceba

    Directory of Open Access Journals (Sweden)

    Borrero Giovanny

    1990-06-01

    Full Text Available En Palmira Colombia se realizó un ensayo utilizando heno de guandul, Cajanus cajan L., para reemplazar concentrado comercial en niveles de 25,50,75 y 100 % con un tratamiento testigo de 100% concentrado comercial. Se emplearon 30 gazapos Nueva Zelanda blancos con una edad de 35 días, distribuídos en un diseño de bloques completamente al azar (BOCA donde el peso fue el factor de bloqueo. Cada bloque de 10 animales. Cada unidad experimental estaba constituida por dos animales alojados en jaulas de 0.3 x 0.6. Cada tratamiento tuvo 3 repeticiones. Se evaluaron las variables ganancia de peso, consumo de materia seca, conversión alimenticia, rendimiento en canal y semanas para alcanzar 2 kg. A medida que aumentó el nivel de guandul disminuyó la ganancia de peso/animal/día y el rendimiento en canal y se necesitó mayor número de semanas para alcanzar los 2 kg de peso. Se registraron mejores resultados en el testigo que en los tratamientos con guandul, sin embargo los resultados del tratamiento del 75 % de concentrado + 25 % de guandul, fueron similares a los del testigo y fue el tratamiento de mayor beneficio neto.In Palmira, Colombia was done an experiment using guandul (Cajanus cajan hay for replacing commercial feed in 25, 50, 75 and 100 % levels with a control treatment, 100 % commercial feed. Thirty white New Zeland rabbits 35 days old , were used and distributed in a Randomized Block Design (RDD where weight was the blocking factor. Each block had 10 animals. Every experimental unit was composed for two rabbits located in one e age (0.3 m x 0.6 m. Each treatment had 3 replications. The following variables were evaluated: weight gain, dry matter consumption, feed efficiency, carcass percentage ad weeks to reach the 2 kg weight. For every increase in guandul hay levels, there was a reduction in daily weight gain and carcass percentage and it was required more time to reach the 2 kg weight. When the control treatment was compared with the

  14. Effect of Agricultural Amendments on Cajanus cajan (Pigeon Pea) and Its Rhizospheric Microbial Communities--A Comparison between Chemical Fertilizers and Bioinoculants.

    Science.gov (United States)

    Gupta, Rashi; Bisaria, V S; Sharma, Shilpi

    2015-01-01

    Inoculation of leguminous seeds with bioinoculants has been practiced in agriculture for decades to ameliorate grain yield by enhanced growth parameters and soil fertility. However, effective enhancement of plant growth parameters results not only from the direct effects these bioinoculants impose on them but also from their non-target effects. The ability of bioinoculants to reduce the application of chemicals for obtaining optimum yield of legume appears to be of great ecological and economic importance. In the present study, we compared the influence of seed inoculation of Cajanus cajan with a microbial consortium, comprising Bacillus megaterium, Pseudomonas fluorescens and Trichoderma harzianum, with that of application of chemical fertilizers on plant's growth parameters and its rhizospheric microbial communities. Real-time PCR assay was carried out to target the structure (16S rRNA) and function (nitrogen cycle) of rhizospheric microbiota, using both DNA and RNA as markers. The results showed that the microbial consortium was the most efficient in increasing grain yield (2.5-fold), even better than the recommended dose of chemical fertilizers (by 1.2-fold) and showed enhancement in nifH and amoA transcripts by 2.7- and 2.0-fold, respectively. No adverse effects of bioinoculants' application were observed over the rhizospheric microbial community, rendering the consortium to be safe for release in agricultural fields. PMID:26231030

  15. Use of Lactobacillus acidophilus and Lactobacillus casei for a potential probiotic legume-based fermented product using pigeon pea (Cajanus cajan).

    Science.gov (United States)

    Parra, K; Ferrer, M; Piñero, M; Barboza, Y; Medina, L M

    2013-02-01

    The aim of the present study was to evaluate the use of pigeon pea (Cajanus cajan) as an appropriate substrate in the production of a legume-based fermented product with Lactobacillus acidophilus ATCC 314 or Lactobacillus casei ATCC 393 and then to ascertain the effects of the addition of ingredients such as powdered milk and banana or strawberry sauce. The products were analyzed for viable cell counts, pH, and sensory attributes during product manufacture and throughout the refrigerated storage period at 3, 7, 14, 21, and 28 days. Nine types of products were produced. At the end of the storage period, the viability of L. acidophilus was above 7 log CFU/g in the presence of milk and 20% sucrose fruit sauce. For products with L. casei, the lack of ingredients such as milk caused no significant loss in viability; however, a high concentration of sucrose in the fruit sauce was an important factor in maintaining a high L. casei population. L. casei had high viability and good sensory attributes. Both strains could be considered suitable for a pigeon pea-based fermented potential probiotic product and a low-cost protein source. PMID:23433374

  16. A New Late-ripening Cajanus cajan Cultivar'CAF5'%晚熟木豆新品种'CAF5'

    Institute of Scientific and Technical Information of China (English)

    万友名; 李正红; 马宏; 刘秀贤; 谷勇; 李立; 梁宁

    2011-01-01

    'CAF5'木豆是运用单株选择和集团选择手段,从云南禄丰县地方栽培品种中选育出的新品种.生育期259~266 d,平均单株荚数(1 176.5±469.2)个,每荚粒数(5.1±0.5)粒,种子百粒质量(7.94±0.58)g,单株粒质量(242.67±95.14)g.%‘CAF5’ is a new cultivar of Cajanus cajan, bred from endemic pigeonpea cultivars of Lufeng, Yunnan Province.Single plant selection and group selection were applied into the breeding course.Its growth period ranges 259 - 266 days and average number of pod per plant is 1 176.5 ± 469.2; Each pod contains (5.1 ±0.5) beans; 100 seeds weight (7.94±0.58) g.Total seed weight per plant is up to (242.67±95.14) g.

  17. Effect of Agricultural Amendments on Cajanus cajan (Pigeon Pea and Its Rhizospheric Microbial Communities--A Comparison between Chemical Fertilizers and Bioinoculants.

    Directory of Open Access Journals (Sweden)

    Rashi Gupta

    Full Text Available Inoculation of leguminous seeds with bioinoculants has been practiced in agriculture for decades to ameliorate grain yield by enhanced growth parameters and soil fertility. However, effective enhancement of plant growth parameters results not only from the direct effects these bioinoculants impose on them but also from their non-target effects. The ability of bioinoculants to reduce the application of chemicals for obtaining optimum yield of legume appears to be of great ecological and economic importance. In the present study, we compared the influence of seed inoculation of Cajanus cajan with a microbial consortium, comprising Bacillus megaterium, Pseudomonas fluorescens and Trichoderma harzianum, with that of application of chemical fertilizers on plant's growth parameters and its rhizospheric microbial communities. Real-time PCR assay was carried out to target the structure (16S rRNA and function (nitrogen cycle of rhizospheric microbiota, using both DNA and RNA as markers. The results showed that the microbial consortium was the most efficient in increasing grain yield (2.5-fold, even better than the recommended dose of chemical fertilizers (by 1.2-fold and showed enhancement in nifH and amoA transcripts by 2.7- and 2.0-fold, respectively. No adverse effects of bioinoculants' application were observed over the rhizospheric microbial community, rendering the consortium to be safe for release in agricultural fields.

  18. Chemical composition of the SFE-CO extracts from Cajanus cajan (L.) Huth and their antimicrobial activity in vitro and in vivo.

    Science.gov (United States)

    Zu, Yuan-gang; Liu, Xiao-lei; Fu, Yu-jie; Wu, Nan; Kong, Yu; Wink, Michael

    2010-12-01

    The in vitro and in vivo antimicrobial activities of SFE-CO₂(supercritical fluid extraction) extracts and ethanol extracts from Cajanus cajan (L.) Huth were investigated. The flavonoid compounds orientin, vitexin, isovitexin, pinostrobin and the stilbene cajaninstilbene acid were detected in SFE-CO₂ extracts by HPLC-DAD. In vitro antimicrobial activities of the extracts were evaluated against eight microbial strains (the bacteria Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Proteus vulgaris, Pseudomonas aeruginosa, Escherichia coli; and the fungi Aspergillus niger and Candida albicans). A marked inhibitory effect of the SFE extracts was observed against Staphylococcus epidermidis, Staphylococcus aureus and Bacillus subtilis. The IC(50) of SFE-CO₂ extracts ranged from 0.0557 mg/ml to 0.0689 mg/ml consisting of cancer (MCF-7 (0.0557 mg/ml)) as well as non-cancer (BHK-21 (0.0641 mg/ml), RAW264.7 (0.0689 mg/ml) and Vero (0.0625 mg/ml)) cells. Flow cytometry (FCM) was used to analyze death rate of the most sensitive strain (Staphylococcus aureus) caused by the SFE extracts. Additionally, the whole cell proteins of Staphylococcus aureus were analyzed by SDS-PAGE to detect if there were changes in protein patterns. In vivo antimicrobial activity was studies in mice that had been inoculated with Staphylococcus aureus. The potential mechanism of antimicrobial activity in vivo was studied by histopathology. PMID:20576412

  19. Assessing the mutagenic effects of gamma irradiation on Cajanus cajan (L. Huth and Vigna unguiculata (L. Walp landraces using morphological markers

    Directory of Open Access Journals (Sweden)

    Ogbuagu Udensi Ugorji

    2012-12-01

    Full Text Available Giving the high adaptability, heritability, genetic variability and nutritive values reported in the indigenous landraces of legumes, combined efforts should be geared towards their improvement.The present study aimed at evaluating the sensitivity of “Fiofio” Cajanus cajan (L. Huth and “Olaudi” Vigna unguiculata (L. Walp to gamma irradiation using morphological markers. Seeds of these legumes were exposed to gamma irradiation at 0, 200, 400, 600, 800 Gy from 60Co source. There was significant effect of gamma irradiation on days to 50% seedling emergence in a dosedependent fashion for “Fiofio” but it did not significantly affect the seedling emergence of “Olaudi”. Germination percentage was not affected concerning the two crops but the survival percentage was significantly reduced as the dose of exposure increased, especially for “Fiofio” variety. Growth and yield traits were increased in plants raised from seeds exposed to 200 Gy of gamma irradiation for both legumes. “Fiofio” plants raised from seeds treated with 400, 600 and 800 Gyhave died after two months, while those of “Olaudi” have presented a retarded growth. Explicitly, exposing the seeds of these legumes to 200 Gy of gamma irradiation could serve as a good springboard for theirimprovement.

  20. 木豆毛状根的诱导及其培养条件的研究%Induction and Culture Condition of Hairy Roots of Cajanus cajan (L.) Millsp.

    Institute of Scientific and Technical Information of China (English)

    于海娣; 王慧梅; 付玉杰

    2012-01-01

    Hairy roots of Cajanus cajan (L. ) Millsp. was induced by Agrobacterium rhizogenes LBA9402 with direct inoculation. The optimum condition of induction of hairy roots in this test was as follows, the leaves of C. cajan were used as explants to be inoculated on 1/2MS solid medium, pre-culture for 2 d, the concentration of bacterium was OD = 0. 6 -0. 8, dissemination for 20 min, co-culture for 3 d, the rate of the hairy roots was 60%. PCR analysis at molecular level proved that T-DNA of Ri plasmid of A. rhizogenes 9402 has been successfully transferred into the genome of hairy roots of C. cajan.%利用发根农杆菌LBA9402对木豆叶片直接进行诱导产生毛状根.本实验研究出诱导木豆毛状根的最佳条件是,以木豆叶片为外植体,于1/2MS固体培养基上预培养2 ~4d,菌液浓度OD600 =0.6 ~0.8,浸染20 min,共培养3d,诱导率为60.00%.在分子水平用PCR检测表明,发根农杆菌9402Ri质粒上的T-DNA成功整合进木豆毛状根的基因组中.

  1. The role of Cajanus cajan for soil erosion control in Southern and development countermeasures%木豆在南方水土流失治理中的作用及发展对策

    Institute of Scientific and Technical Information of China (English)

    徐晓俞; 李爱萍; 郑菲艳; 郑开斌

    2015-01-01

    木豆在南方水土流失治理中具有重要的作用.综述木豆的特征特性,分析其在南方水土流失治理中的应用价值,并提出一些可行的对策措施.%Cajanus cajan played an important role for soil erosion control in southern,its characteristics was reviewed,its application value of soil erosion control was analgzed in southern,and some feasible development countermeasures were put forward.

  2. Comparative study of antidiabetic activity of Cajanus cajan and Tamarindus indica in alloxan-induced diabetic mice with a reference to in vitro antioxidant activity

    Directory of Open Access Journals (Sweden)

    Laizuman Nahar

    2014-01-01

    Full Text Available Background: Oxidative stress not only develops complications in diabetic (type 1 and type 2 but also contributes to beta cell destruction in type 2 diabetes in insulin resistance hyperglycemia. Glucose control plays an important role in the pro-oxidant/antioxidant balance. Some antidiabetic agents may by themselves have antioxidant properties independently of their role on glucose control. Objective: The present investigation draws a comparison of the protective antioxidant activity, total phenol content and the antihyperglycemic activity of the methanolic extract of Cajanus cajan root (MCC and Tamarindus indica seeds (MTI. Materials and Methods: Antidiabetic potentials of the plant extracts were evaluated in alloxan-induced diabetic Swiss albino mice. The plant extracts at the doses of 200 and 400 mg/kg body weight was orally administered for glucose tolerance test during 1-hour study and hypoglycemic effect during 5-day study period in comparison with reference drug Metformin HCl (50 mg/kg. In vitro antioxidant potential of MCC and MTI was investigated by using 1, 1- diphenyl-2-picrylhydrazyl (DPPH free radical scavenging activity at 517 nm. Total phenolic content, total antioxidant capacity and reducing power activity was also assayed. Results: There was a significant decrease in fasting serum glucose level (P < 0.001, reduction in blood glucose level (P < 0.001 in 5-days study, observed in the alloxan-induced diabetic mice. The reduction efficacy of blood glucose level of both the extracts is proportional to their dose but MCC is more potent than MTI. Antioxidant study and quantification of phenolic compound of both the extracts revealed that they have high antioxidant capacity. Conclusion: These studies showed that MCC and MTI have both hypoglycemic and antioxidant potential but MCC is more potent than MTI. The present study suggests that both MCC and MTI could be used in managing oxidative stress.

  3. Identification of microRNA in Cajanus cajan based on EST and GSS Sequences%基于EST和GSS序列的木豆miRNA生物信息学分析

    Institute of Scientific and Technical Information of China (English)

    李崇奇; 周鹏; 蔡望伟

    2014-01-01

    应用miRtour在线分析工具对木豆EST和GSS序列进行miRNA生物信息学预测,应用psRNATarget进行靶基因预测。结果发现,43条不同的木豆miRNA成熟序列,隶属于33个不同的miRNA家族。靶基因预测发现有36条编码序列受miRNA调控,其中17条序列编码酶,2条编码转录因子,7条编码参与细胞信号转导的蛋白,5条编码参与蛋白质降解通路的蛋白。%Cajanus cajan microRNA has been predicted based on EST and GSS Sequences by miRtour, whereas miRNA-targeted mRNAs has been predicted by psRNATarget. 43 unique mature miRNA sequenc-es were found belonging to 33 different miRNA familes in Cajanus cajan. 36 coding RNAs were regulated by miRNA in which 17 targets were enzymes,2 targets were transcription factors,7 targets were protein in-volved in signaling transduction,5 targets were protein participated in protein degradation pathway.

  4. Assessment of beneficial role of an insectivorous bird, jungle babbler (Turdoides striatus predation, on Helicoverpa armigera infesting pigeon pea (Cajanus cajan crop

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    Bhavna Bharucha

    2010-04-01

    Full Text Available Jungle babbler (Turdoides striatus, a widely spread sub-tropical insectivorous passerine is considered beneficial to agro-ecosystem, as they devour voraciously on insect matter especially Helicoverpa armigera, the gram pod borer, a notorious pest infesting and causing heavy loses to crops like pigeon pea (Cajanus cajan which is a vital crop of semi-arid tropical and subtropical farming system, providing high quality vegetable protein. Helicoverpa is known to feed on flowers, pods, and seeds and is the most important biotic constraint affecting pigeon pea yields. Jungle babblers have a peculiar foraging style which helps expose the Helicoverpa larvae as well as pupae through various phenological stages of pigeon pea. For comparative assessment of their beneficial role and as a possible bio control agent, in Baroda city (State of Gaujarat, India, was studied, two crops of pigeon pea (insecticide treated and untreated (control were selected. In both treated and control crops, the number of jungle babblers were maximum in pigeon pea fields during october and november in both small pod stage and large pod stage which had heavy infestation of Helicoverpa. Least number of birds was seen during the flowering stage in September. Later in treatment crop three applications of Dunnate and Monocrotophos insecticide spray was done after which the pest population decreased which is reflected in number of birds in the field, while the bird number in control crops grew since insecticide spray was not done and number of larvae increased with the stage of the crop. Along with the main crop pigeon pea, comparative study was also done to see the food preference by these birds in crops like sorghum, maize, cow pea and ploughed and unploughed fields. Maximum number of birds was seen in unploughed field and least in sorghum suggesting that Helicoverpa is preferred food over sorghum grains thus pigeon pea and sorghum can be used as mixed crops to protect the crop from

  5. Assessment of beneficial role of an insectivorous bird, jungle babbler (Turdoides striatus predation, on Helicoverpa armigera infesting pigeon pea (Cajanus cajan crop

    Directory of Open Access Journals (Sweden)

    Bharucha Bhavna

    2010-06-01

    Full Text Available Jungle babbler (Turdoides striatus, a widely spread sub-tropical insectivorous passerine is considered beneficial to agro-ecosystem, as they devour voraciously on insect matter especially Helicoverpa armigera, the gram pod borer, a notorious pest infesting and causing heavy loses to crops like pigeon pea (Cajanus cajan which is a vital crop of semi-arid tropical and subtropical farming system, providing high quality vegetable protein. Helicoverpa is known to feed on flowers, pods, and seeds and is the most important biotic constraint affecting pigeon pea yields. Jungle babblers have a peculiar foraging style which helps expose the Helicoverpa larvae as well as pupae through various phenological stages of pigeon pea. For comparative assessment of their beneficial role and as a possible bio control agent, in Baroda city (State of Gaujarat, India, was studied, two crops of pigeon pea (insecticide treated and untreated (control were selected. In both treated and control crops, the number of jungle babblers were maximum in pigeon pea fields during october and november in both small pod stage and large pod stage which had heavy infestation of Helicoverpa. Least number of birds was seen during the flowering stage in September. Later in treatment crop three applications of Dunnate and Monocrotophos insecticide spray was done after which the pest population decreased which is reflected in number of birds in the field, while the bird number in control crops grew since insecticide spray was not done and number of larvae increased with the stage of the crop. Along with the main crop pigeon pea, comparative study was also done to see the food preference by these birds in crops like sorghum, maize, cow pea and ploughed and unploughed fields. Maximum number of birds was seen in unploughed field and least in sorghum suggesting that Helicoverpa is preferred food over sorghum grains thus pigeon pea

  6. Assessment of beneficial role of an insectivorous bird, jungle babbler (Turdoides striatus predation, on Helicoverpa armigera infesting pigeon pea (Cajanus cajan crop Evaluación del rol benéfico de Turdoides striatus como predator de Helicoverpa armigera en el cultivo de guandul (Cajanus cajan

    Directory of Open Access Journals (Sweden)

    Bhavna Bharucha

    2010-04-01

    Full Text Available Jungle babbler (Turdoides striatus, a widely spread sub-tropical insectivorous passerine is considered beneficial to agro-ecosystem, as they devour voraciously on insect matter especially Helicoverpa armigera, the gram pod borer, a notorious pest infesting and causing heavy loses to crops like pigeon pea (Cajanus cajan which is a vital crop of semi-arid tropical and subtropical farming system, providing high quality vegetable protein. Helicoverpa is known to feed on flowers, pods, and seeds and is the most important biotic constraint affecting pigeon pea yields. Jungle babblers have a peculiar foraging style which helps expose the Helicoverpa larvae as well as pupae through various phenological stages of pigeon pea. For comparative assessment of their beneficial role and as a possible bio control agent, in Baroda city (State of Gaujarat, India, was studied, two crops of pigeon pea (insecticide treated and untreated (control were selected. In both treated and control crops, the number of jungle babblers were maximum in pigeon pea fields during october and november in both small pod stage and large pod stage which had heavy infestation of Helicoverpa. Least number of birds was seen during the flowering stage in September. Later in treatment crop three applications of Dunnate and Monocrotophos insecticide spray was done after which the pest population decreased which is reflected in number of birds in the field, while the bird number in control crops grew since insecticide spray was not done and number of larvae increased with the stage of the crop. Along with the main crop pigeon pea, comparative study was also done to see the food preference by these birds in crops like sorghum, maize, cow pea and ploughed and unploughed fields. Maximum number of birds was seen in unploughed field and least in sorghum suggesting that Helicoverpa is preferred food over sorghum grains thus pigeon pea and sorghum can be used as mixed crops to protect the crop from

  7. 木豆CGMS杂交种生产中的传粉昆虫%Insect Pollinators in CGMS Hybrid Seed Production of Cajanus cajan

    Institute of Scientific and Technical Information of China (English)

    李正红; 梁宁; 马宏; Kul Bhushan SAXENA; 刘秀贤; 宗绪晓

    2011-01-01

    木豆是世界上唯一的木本食用豆类作物,具有较强的抗旱性.木豆杂交制种需昆虫传粉,制种地传粉昆虫的种类及数量是制种产量的决定性因素.本文调查了云南省元谋县CGMS木豆[Cajanuscajan(L.)Millspaugh]杂交种ICPH2671制种田访花昆虫种类、数量、访花频次及杂交种制种产量.结果表明,在云南元谋,木豆访花昆虫共有5目15科25种,其中主要5种传粉媒介为绒切叶蜂(Megachilevelutina)、切叶蜂属1种(Megachilesp5)、圆柄木蜂(Xylocopatenuiscapa)、蜜蜂亚科1种(Apinaesp1)和切叶蜂属1种(Megachilesp2).木豆盛花期,制种亲本不育系每个分枝昆虫访花频次为每10min2.8次,而恢复系植株上的访花频次为每10min5.2次,表明昆虫对恢复系花朵有明显偏好;在前述昆虫访花频次下,不育系单株干籽粒产量达383.7g,恢复系单株产量为357.0g,二者无显著差异,说明这样的昆虫访问频次已能满足杂交制种中将花粉由恢复系传至不育系的需要.%Pigeonpea [Cajanus cajan (L.) Millspaugh] is the only shrubby food legume crop in the world, with drought tolerance. Insect pollinators are essential on flower pollination in CGMS pigeonpea lines, and their species, abundance, visiting frequency are the key factors for pigeonpea hybrid production. More than 46 species of insects were reported as flower visitors in the open field for pigeonpea production outside China, andMegachile spp. Were the major pollinators. Reports on relations between insect pollinators and pigeonpea hybrid production were seldom in the world. In China, there is not any research report on relation between CGMS hybrid production of pigeonpea and its insect pollinators. Therefore, species, abundance, visiting frequency of flower-visiting insects at flowering stage and hybrid yield of pigeonpea were investigated recently, in pigeonpea hybrid production field in Yuanmou County in Yunnan province of China, using CGMS based ICPH2671 hybrid

  8. Study of the mineral variation of two varieties of pigeonpea (Cajanus cajan (L.) Millsp) submitted to different doses of fertilizers by the neutron activation analysis; Acompanhamento da variacao mineral de duas cultivares de guandu (Cajanus cajan (L.) Millsp) submetidas a diferentes doses de fertilizantes, pelo metodo de analise por ativacao com neutrons

    Energy Technology Data Exchange (ETDEWEB)

    Piasentin, Ricardo Marcelo

    2001-07-01

    Instrumental neutron activation analysis (INAA) followed by gamma-ray spectrometry was used to determine the concentrations of As, Ca, Co, Cr, Cu, Eu, Fe, K, La, Mg, Mn, Mo, Na, Pr, Sb, Se, Th, U, V and Z, in 82 leaf samples belonging to two cultivars of pigeonpea (Cajanus cajan (L.) Millsp). Pigeonpea, an easy cultivation legume species, probably originated from Africa and adapted to brazilian conditions, is a very promising and unconventional source of food, mainly to the grain production intended for human feeding, and that even can be used in flour products. It is an excellent proteic supplement for ruminants, due to its large potential of forage production and its high nutritive value, or in addition it can be used in the recuperation of exhausting soils either by means of its utilization as green manure or by taking free Nitrogen from the atmosphere to produce nitrates which can be utilized by the plants. However, in spite of the several possibilities and increasing utilization of pigeonpea, data about the composition of microelements and trace elements of this forage species ate scarce. The plants employed in this work were submitted, individually, to two different doses of fertilizers containing the following elements: B, Co, Cu, Fe, Mn, Mo, V and Zn. The leaf samples were harvested in two different times at 4-month intervals. Statistics was used to verify the difference promoted on the concentrations of those 20 elements determined, due to the use of different fertilizers in relation to the plants which were not submitted to any fertilizer, the so-called test or control plants, as well as, to compare the eventual variations in the concentration of the elements of interest due to the leaf harvest time, due to both doses of fertilizers and between both varieties of pigeonpea studied. Experimental designs were applied to evaluate the effect promoted by possible interactions related to the leaf harvest time, mineral fertilizer doses and the use or not of

  9. AVALIAÇÃO FÍSICA, QUÍMICA, BIOQUÍMICA E AGRONÔMICA DE CULTIVARES DE FEIJÃO-GUANDU (CAJANUS CAJAN (L MILL

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    SOLANGE GUIDOLIM CANNIATTI-BRAZACA

    2009-07-01

    Full Text Available

    RESUMO: Foram estudadas as características físicas, químicas, bioquímicas e agronômicas de 40 cultivares de feijão-guandu (Cajanus cajan (L Mill provenientes da EMBRAPA de São Carlos (SP e originárias de diversas regiões do Brasil e da Índia. Pode-se observar entre os cultivares variações expressivas no tempo de cocção, no teor de lectinas e na produtividade. Os resultados obtidos quanto ao teor protéico e à digestibilidade do guandu, quando comparadas com os resultados obtidos na literatura, mostraram-se semelhantes aos dos grãos de feijão-comum. Pode-se, também, observar baixos teores de taninos entre os cultivares estudados. Foram selecionados três cultivares por apresentarem melhores características entre os parâmetros estudados. PALAVRAS-CHAVE: Feijão-guandu; proteínas; fatores antinutricionais; produtividade.

  10. Effects of Exogenous Calcium on the Physiological Characters of Cajanus cajan under Drought Stress%外源钙对干旱胁迫下木豆种苗生理特性的影响

    Institute of Scientific and Technical Information of China (English)

    陈莹; 王普昶; 赵丽丽; 杨春燕; 宋高翔

    2014-01-01

    通过模拟贵州岩溶地区土壤富钙的生境(CK和50 mmol·L-1 CaCl2),以岩溶地区常见饲用灌木木豆(Cajanus cajan)为材料,研究外源钙对干旱胁迫(CK,5%,10%,20% PEG-6000)下木豆种子的萌发率、保护性酶活性(SOD,POD,CAT)及可溶性蛋白质和GSH含量的变化.结果表明:木豆种子萌发随着干旱胁迫的增强而呈显著线性下降趋势(P<0.01),施加外源钙对其无显著影响;随着干旱胁迫的增强保护酶SOD和POD成指数关系,CAT成负二次关系;施加外源钙能显著提高低浓度干旱胁迫下木豆种子SOD和POD活性;可溶性蛋白质和GSH含量随着干旱胁迫的增强呈现指数和二次曲线关系,施加外源钙显著提高了木豆种子可溶性蛋白质和GSH含量(P<0.05).表明外源钙的施加能缓解木豆种子受到的干旱伤害,提高木豆苗期抗旱性.

  11. 低磷胁迫下木豆利用磷的基因型差异及其机制%Genotypic variation in putilization of Cajanus Cajan (L.) Millsp under low-p stress and corresponded mechanisms

    Institute of Scientific and Technical Information of China (English)

    秦丽凤; 玉永雄; 黎晓峰; 顾明华

    2007-01-01

    以木豆[Cajanus Cajan (L.) Millsp] MD2、MD5、MD7为材料,采用水培试验方法研究了木豆对磷的吸收、利用的基因型差异及其机理.结果表明:低磷(P 1 ìmol·L-1)胁迫下,木豆对磷素的吸收和利用效率存在明显的基因型差异,其中MD2、MD7属于磷素吸收、利用效率较强的基因型,而MD5为磷素效率较低的基因型.低磷培养16 d后,磷高效的基因型的根/冠比显著增加,根分泌的酸性磷酸酶(APA)活性较高,说明木豆对磷素的吸收效率与植株的根冠比及根分泌的酸性磷酸酶活性有关.在低磷胁迫下,虽然MD7、MD2的含磷量较低,但是磷素利用效率和根尖中APA活性较高,说明根中高APA活性是磷利用效率高的重要原因之一.

  12. Influência de fatores antinutricionais da leucena (Leucaena leucocephala e Leucaena cunningan e do feijão guandu (Cajanus cajan sobre o epitélio intestinal e o desempenho de frangos de corte

    Directory of Open Access Journals (Sweden)

    Oliveira Paulo Batista de

    2000-01-01

    Full Text Available Um experimento foi conduzido para avaliar a influência de fatores antinutricionais de alguns alimentos sobre o epitélio intestinal e o desempenho de frangos de corte. Foram utilizados 480 pintos de um dia de idade, em um delineamento inteiramente casualizado com quatro tratamentos (A = ração testemunha à base de milho e soja; B = ração contendo Leucaena leucocephala - 6% [LL]; C = ração contendo Leucaena cunningan - 6% [LC] e D = ração contendo feijão guandu - Cajanus cajan - 20% [CC], quatro repetições e 30 aves por unidade experimental. Houve redução de ganho de peso e aumento da conversão alimentar com a utilização de LL e LC, no período de 1a 21 e de 1a 42 dias de idade. Aos 21 dias de idade, observou-se aumento no tamanho dos vilos do jejuno e na relação vilo:cripta do jejuno e íleo, nas aves que receberam LC. Houve aumento significativo (P<0,05 das células caliciformes no duodeno e jejuno em aves de todos os tratamentos e apenas LL e LC aumentaram as células caliciformes do íleo. Aos 42 dias de idade, houve aumento no número de células caliciformes causado por CC no duodeno e por LL, LC e CC no íleo. O consumo de ração e a profundidade de cripta não foram influenciados. A alteração da morfologia da vilosidade do jejuno, bem como a alteração na relação vilo:cripta no período inicial, influenciou negativamente o ganho de peso e a conversão alimentar nos períodos inicial e total.

  13. 木豆叶醇提物对皮质酮诱导的PC12细胞损伤的保护作用%Protective Effect of Alcohol Extract of Cajanus cajan on Corticosterone-induced Lesion in Cultured PC12 Cells

    Institute of Scientific and Technical Information of China (English)

    姜保平; 刘亚旻; 李宗阳; 宋波; 潘瑞乐

    2012-01-01

    建立皮质酮诱导的PC12细胞损伤模型并观察木豆叶醇提物及不同组分对皮质酮损伤PC12细胞的保护作用.以100μ mol/L的皮质酮诱导PC12细胞损伤;损伤后的PC12细胞与木豆叶醇提物及不同组分孵育24h,通过形态学观察、MTT检测、LDH测定,研究各组分对皮质酮损伤PC12细胞的保护作用.结果表明,PC12细胞与皮质酮孵育48 h后细胞存活率明显降低,而LDH水平显著升高.而加入木豆叶醇提物及各组分时上述效果明显减轻,且存在明显的剂量依赖关系.从以上结果可知,木豆叶醇提物及不同组分对皮质酮损伤的PC12细胞均有保护作用,且醇提物的效果最好.%The aim of this paper was to establish the corticosterone-induced damage model in PC12 cells and study the protective activity of alcohol extracts and its fractions from Cajanus cajan on corticosterone-induced PC12 cells. PC12 cells were exposed to different concentrations of corticosterone to determine the optimum concentration of establishing the damage model,and then the injured PC12 cells were incubated with the alcohol extract and its fractions for 24 h. After that the neuroprotective effect on injured PC12 cells was investigated by morphological observation, LDH detection and MTT determination. The results showed that the survival rate of PC12 cells and the cells exposed to corticosterone (100 (μmol/L) for 48 h decreased obviously,with an increase in lactate dehydrogenase (LDH) level,but the effect became weak when the alcohol extracts and its fractions from the C. cajan was added to the PC 12 cells,and it was in a dose-dependent manner. The author suggests that the alcohol extract and its fractions from C. cajan can generate a neuroprotective effect on injured PC 12 cells induced by corticosterone,and that of alcohol extract is the most significant.

  14. Growth of seedlings of pigeon pea (Cajanus cajan (l. millsp, wand riverhemp (Sesbania virgata (cav. pers., and lead tree (Leucaena leucocephala (lam. de wit in an arsenic-contaminated soil

    Directory of Open Access Journals (Sweden)

    Luiz Eduardo Dias

    2010-06-01

    Full Text Available Phytoremediation strategies utilize plants to decontaminate or immobilize soil pollutants. Among soil pollutants, metalloid As is considered a primary concern as a toxic element to organisms. Arsenic concentrations in the soil result from anthropogenic activities such as: the use of pesticides (herbicides and fungicides; some fertilizers; Au, Pb, Cu and Ni mining; Fe and steel production; coal combustion; and as a bi-product during natural gas extraction. This study evaluated the potential of pigeon pea (Cajanus cajan, wand riverhemp (Sesbania virgata, and lead tree (Leucaena leucocephala as phytoremediators of soils polluted by As. Soil samples were placed in plastic pots, incubated with different As doses (0; 50; 100 and 200 mg dm-3 and then sown with seeds of the three species. Thirty (pigeon pea and 90 days after sowing, the plants were evaluated for height, collar diameter and dry matter of young, intermediate and basal leaves, stems and roots. Arsenic concentration was determined in different aged leaves, stems and roots to establish the translocation index (TI between the plant root system and aerial plant components and the bioconcentration factors (BF. The evaluated species showed distinct characteristics regarding As tolerance, since the lead tree and wand riverhemp were significantly more tolerant than pigeon pea. The high As levels found in wand riverhemp roots suggest the existence of an efficient accumulation and compartmentalization mechanism in order to reduce As translocation to shoot tissues. Pigeon pea is a sensitive species and could serve as a potential bioindicator plant, whereas the other two species have potential for phytoremediation programs in As polluted areas. However, further studies are needed with longer exposure times in actual field conditions to reach definite conclusions on relative phytoremediation potentials.A fitorremediação é uma estratégia que utiliza plantas para descontaminar ou imobilizar poluentes

  15. 木豆叶总芪对高脂模型兔的降脂作用及调节机制%Hypocholesterolemic effect of total stilbene from Cajanus cajan L.in hyperlipidemic rabbit model and regulatory mechanism

    Institute of Scientific and Technical Information of China (English)

    郭显蓉; 骆庆峰; 康晓敏; 尹洪超; 斯建勇

    2011-01-01

    目的 研究木豆叶总芪(TSC)对高脂模型兔脂质代谢的影响及可能的分子机制.方法 将36只雄性新西兰兔分为对照,模型,TSC高、中、低刺量和血脂康6组,于0、2和6周末测血脂,6周末测肝脏脂质并行病理学检测;将THP-1细胞诱导成巨噬细胞,用[H]胆固醇预处理48 h后分为对照、木豆叶总芪单体成分C03高、中、低剂量和雌二醇5组,作用24 h后加入apoAI作用12 h,检测各组上清液和细胞内[H]胆固醇含量,计算细胞胆固醇流出率,Western blot检测各组细胞ABCA1表达.结果 与模型组相比,TSC高剂量使兔血清和肝脏TC分别下降35.36%和35.97%(P<0.01),TG分别下降12.34%(P<0.05)和41.32%(P<0.01),血清LDL-C下降29.07%(P<0.01);HE染色:TSC高剂量组肝脂肪病变明显轻于模型组.C03高、中剂量作用后细胞胆固醇外流率分别提高55.39%和24.97%(P<0.01).C03高剂量使ABCA1蛋白表达显著增加.结论 TSC能抑制高脂饮食所致高脂血症,其降脂作用可能与上调细胞ABCA1表达,进而促进胆固醇逆转运有关.%Objective To investigate the potential hypolipidemic effects of total stilbene from Cajanus cajan L.(TSC) in rabbits fed with a high-lipid diet and the mechanism of regulation of cholesterol metabolism.Methods Thirty-six male rabbits were divided into six groups: control group, high lipid model group, TSC-treated with 200, 100,50 mg/kg, and Xuezhikang treated group.The effects of TSC were investigated by monitoring serum and liver lipid profile in rabbits.THP-1 cells were induced to macrophages by PMA.The macrophages were labeled with [3H]cholesterol.These cells were divided into five groups, i.e, normal group, Cajanin high concentration group (CH), Cajanin mean concentration group (CM), Cajanin low concentration group (CL) and estradiol (E2) group.The cells were cultured for 24 hours in the serum-free RPMI 1640 medium containing 2mg/ml bovine serum albumin(BSA) in the presence or absence of Cajanin ( 30

  16. 木豆在干热河谷退化山地的生态适应性研究%The study on the ecological adaptability of Cajanus cajan (L.) Millspaugh in the degraded mountain land of dry-hot valley

    Institute of Scientific and Technical Information of China (English)

    纪中华; 杨艳鲜; 拜得珍; 方海东; 廖承飞; 潘志贤; 沙毓沧

    2007-01-01

    2000年木豆(Cajanus cajan(L.)Millspaugh)引入云南省元谋干热河谷地区,观测其物候特征、生长适应性、抗旱适应性、抗光热适应性等植物学指标,以了解其对干热退化山地的整体生态适应性.结果表明,木豆能适应干热河谷的退化山地生长环境,其生长势优于原产地,表现出明显的速生性;木豆的抗光热能力较强,能适应于热河谷的不同光照及高温环境,但温度高于37℃,则光合作用停止;木豆的抗旱能力较强,能够通过不同的生理适应性度过旱季,完成其生长周期.木豆适应于干热河谷的生态环境,利用价值较大,是干热河谷退化山地治理与开发的优选灌木.

  17. Accumulation effects on function of the high-brush forest compound model of the 'Tamarindus+Cajanus cajan'%"罗望子+木豆"乔灌复合模式林水土保持功能的叠加效应初探

    Institute of Scientific and Technical Information of China (English)

    拜得珍; 纪中华; 沙毓沧; 赵艳萍; 潘志贤

    2006-01-01

    以降水等级为主要影响因子,研究了罗望子(Tamarindus indica)+木豆(Cajanus cajan)乔灌复合模式林对降雨的截留、贮存所产生的水保效应.结果表明:罗望子人工林冠层和复合木豆人工灌木林林冠层年截留降雨量分别为97.99和145.4 mm,分别占全年降雨量水的17.48 %和25.86 %;罗望子人工林的树干茎流率占大气降水的2.37 %;复合模式林枯枝落叶的蓄积量为7.08 t/hm2,对降水的平均截留率为12.47 %,其最大持水量相当于1.8 mm的降水;模式林表层土壤(0~40 cm)滞留水分能力相当于2.7 mm降雨,贮存土壤水分能力可达1553.2 t/hm2,产生径流的最小降雨量为35.4 mm,其侵蚀模数和径流模数比对照减少98.52 %和93.21 %,体现出了良好的水土保持和水源涵养效应.

  18. Effects of hydrophobic fraction of Cajanus cajan (L.) Millspaugh on bone density and blood lipid level in obese and diabetic mice%树豆脂溶性部位对糖尿病和肥胖小鼠骨密度及血脂水平的影响

    Institute of Scientific and Technical Information of China (English)

    叶贵福; 王璐; 杨瑞仪; 田汝华; 胡英杰; 沈小玲

    2013-01-01

    Aim To investigate the effect of hydrophobic fraction of Cajanus cajan ( L. ) Millspaugh ( SDE ) on bone density and blood lipid level in diabetic and obese mice. Methods The db/db congenital diabetic mice and ob/ob congenital obese mice were orally administered with SDE for 8 weeks, bone density as well as serum TG, TC, LDL-C, HDL-C, ALP and Ca2 + were measured. Results Compared to mice treated with vehicle, ( 1 ) SDE-treated db/db and ob/ob mice had greater bone density ( P < 0. 05 ) and lower serum ALP level ( P <0. 01 ), with serum Ca2+ level relatively unchanged; ( 2 ) SDE-treated db/db mice had relatively unchanged blood lipid level, but SDE-treated ob/ob mice had significantly lower levels of TG ( P < 0. 05 ), TC ( P < 0. 01 ) and LDL-C ( P < 0. 01 ). Conclusion SDE increased bone density in diabetic and obese mice, and improved lipid metabolism in obese mice.%目的 探讨树豆脂溶性部位(SDE)对糖尿病和肥胖小鼠骨密度及血脂水平的影响.方法 db/db先天糖尿病小鼠和ob/ob先天肥胖小鼠连续8周灌服SDE后,进行骨密度、血清碱性磷酸酶(ALP)、血钙(Ca2+)及血脂水平测定.结果 与灌服溶剂空白的模型组小鼠相比,(1)灌服SDE的db/db和ob/ob小鼠胫骨骨密度明显增加(P<0.05),血清ALP水平明显下降(P<0.01),但血钙水平无明显改变;(2) 灌服SDE的db/db小鼠血脂水平无明显变化,灌服SDE的ob/ob小鼠血清甘油三酯(TG)水平明显下降(P<0.05),血清总胆固醇(TC)和血清低密度脂蛋白胆固醇(LDL-C)水平明显下降(P<0.01).结论 SDE能提高糖尿病和肥胖小鼠骨密度,并能改善肥胖小鼠的血脂代谢.

  19. Genetic variability in pigeonpea (Cajanus cajan L. Millspaugh

    Directory of Open Access Journals (Sweden)

    A.Hamid. A. Husna, M. M. Haque and M. R. Islam

    2011-03-01

    Full Text Available One hundred germplasm lines were evaluated for variation in morphological and agronomic traits for selection of cultivarpotentially suitable for growing in Bangladesh under rice-based cropping systems during the fallow period after kharif II season.A wide range of variation was found in twelve quantitative plant traits. The most important correlations corresponded to eightplant traits. Considering these traits, PCA could explain 76.2% of total variance. Based on cluster analysis, the genotypes weregrouped into seven clusters, each cluster with greater similarities. Plant height, days to flowering and pods plant-1 were thediscriminating variables mostly contributed in grouping the genotypes. Pods plant-1 played the most dominant role in explainingthe maximum variance according to DFA. Genotypes grouped in 1, 4 and 7 clusters were early maturing and high yielder ascompared to the other cluster members. Genotype 21 (ICP7143 representing group 1 showed promising for its short staturenature. However, genotype 32 (ICP7989 representing group 4 produced the highest yield with the shortest maturity duration.These two genotypes are particularly promising for growing in fallow period after the harvest of transplanted aman or kharif IIcrops.

  20. New microsatellite markers for pigeonpea (cajanus cajan (L. millsp.

    Directory of Open Access Journals (Sweden)

    Gebhardt C

    2009-03-01

    Full Text Available Abstract Background Pigeonpea is a nutritious tropical legume with several desirable characteristics but has been relatively neglected in terms of research. More efficient improvement can be achieved in this crop through molecular breeding but adequate molecular markers are lacking and no linkage map has been developed so far. Microsatellites remain the markers of choice due to their high polymorphism and their transferability from closely related genera. The overall objective of this study was to develop microsatellite markers from an enriched library of pigeonpea as well as testing the transferability of soybean microsatellites in pigeonpea. Results Primers were designed for 113 pigeonpea genomic SSRs, 73 of which amplified interpretable bands. Thirty-five of the primers revealed polymorphism among 24 pigeonpea breeding lines. The number of alleles detected ranged from 2 to 6 with a total of 110 alleles and an average of 3.1 alleles per locus. GT/CA and GAA class of repeats were the most abundant di-nucleotide and tri-nucleotide repeats respectively. Additionally, 220 soybean primers were tested in pigeonpea, 39 of which amplified interpretable bands. Conclusion Despite the observed morphological diversity, there is little genetic diversity within cultivated pigeonpea as revealed by the developed microsatellites. Although some of the tested soybean microsatellites may be transferable to pigeonpea, lack of useful polymorphism may hinder their full use. A robust set of markers will still have to be developed for pigeonpea genome if molecular breeding is to be achieved.

  1. Dormência em sementes de guandu (Cajanus cajan (L.) Millsp) Dormancy on pigeon pea seeds (Cajanus cajan (L.) Millsp)

    OpenAIRE

    Rodolfo Godoy; Francisco Humberto Dübbern de Souza

    2004-01-01

    O ensaio foi conduzido para estudar a ocorrência de dormência, expressa em porcentagem de sementes duras, em sementes de 17 linhagens e três cultivares comerciais de guandu. As sementes foram colhidas, debulhadas manualmente e armazenadas em sacos de papel em câmara a 10°C e 25% de umidade relativa do ar. Foram efetuados testes de germinação com quatro repetições de 50 sementes, após um, dois, três, quatro, cinco, nove, doze e quinze meses de armazenamento. Não foram observadas sementes d...

  2. Dormência em sementes de guandu (Cajanus cajan (L. Millsp Dormancy on pigeon pea seeds (Cajanus cajan (L. Millsp

    Directory of Open Access Journals (Sweden)

    Rodolfo Godoy

    2004-12-01

    Full Text Available O ensaio foi conduzido para estudar a ocorrência de dormência, expressa em porcentagem de sementes duras, em sementes de 17 linhagens e três cultivares comerciais de guandu. As sementes foram colhidas, debulhadas manualmente e armazenadas em sacos de papel em câmara a 10°C e 25% de umidade relativa do ar. Foram efetuados testes de germinação com quatro repetições de 50 sementes, após um, dois, três, quatro, cinco, nove, doze e quinze meses de armazenamento. Não foram observadas sementes duras em oito linhagens e nas três cultivares comerciais. Entre essas, a linhagem g58-95 apresentou baixo potencial de armazenamento, com queda rápida de seu poder germinativo. Por outro lado, a linhagem g184-97 apresentou baixo percentual de sementes duras, as linhagens g3-94, g6-95 e g124-95 praticamente mantiveram os percentuais iniciais, as g101-97, g127-97, g154-95 e g167-97 apresentaram aumento no percentual de sementes duras até nove meses de armazenamento e a g27-94, constante decréscimo. Concluiu-se que genótipos de guandu variam amplamente entre si quanto ao caráter 'produção de sementes duras' e que a germinação de suas sementes pode depender de escarificação química ou mecânica.An experiment was conducted to study seed dormancy (hardseedness in seventeen genetic lines and three commercial cultivars of pigeon pea. The seeds were hand-harvested, hand-shelled, and stored in paper bags in a chamber with 10°C and 25% air relative humidity. Four replications of fifty-seed samples were put to germinate in moist paper rolls at 30ºC, after one, two, three, four, five, nine, twelve and fifteen months of storage. No hard seeds were observed among eight lines and the three commercial cultivars. Among these lines, g58-95 had low storage potential, quickly losing germination percentage. Line g184-97 presented low percentage of hard seeds, lines g3-94, g6-95 and g124-95 kept the initial percentage, g101-97, g127-97, g154-95 and g167-97 had the percentage of hard seeds increased from one to nine months of storage, and percentage of hard seeds of line g27-94, constantly decreased. It was concluded that pigeon pea genotypes vary amply among themselves in terms of hard seed production potential and that satisfactory germination of its seed lots may require artificial chemical or mechanical scarification.

  3. Purification, crystallization and preliminary X-ray analysis of urease from pigeon pea (Cajanus cajan)

    Energy Technology Data Exchange (ETDEWEB)

    Balasubramanian, Anuradha; Ponnuraj, Karthe, E-mail: pkarthe@hotmail.com [Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600 025 (India)

    2008-07-01

    Urease from pigeon pea was purified and crystallized and X-ray diffraction data were collected at 2.5 Å resolution. Urease is a seed protein that is common to most Leguminosae. It also occurs in many bacteria, fungi and several species of yeast. Urease catalyzes the hydrolysis of urea to ammonia and carbon dioxide, thus allowing organisms to use exogenous and internally generated urea as a nitrogen source. Urease from pigeon pea seeds has been purified to electrophoretic homogeneity using a series of steps involving ammonium sulfate fractionation, acid precipitation, ion-exchange and size-exclusion chromatography techniques. The pigeon pea urease was crystallized and the resulting crystals diffracted to 2.5 Å resolution. The crystals belong to the rhombohedral space group R32, with unit-cell parameters a = b = 176.29, c = 346.44 Å.

  4. Studies on dhal recovery from pre-treated pigeon pea ( Cajanus cajan L .) cultivars.

    Science.gov (United States)

    Hiregoudar, Sharanagouda; Sandeep, T N; Nidoni, Udaykumar; Shrestha, Bijay; Meda, Venkatesh

    2014-05-01

    Dhal recovery from three popular varieties of North Karnataka was studied using CFTRI mini dhal mill with five different treatments at three different levels. It was observed that Gulyal variety treated with mustard oil recorded maximum hulling efficiency (79.4%) and finished product (68.8%) when compared to a Maruti and Asha variety. However, acetic acid treatment recorded higher hulling efficiency (76.5%) for Maruti followed by Asha (56.9%). The plant growth promoting rhizobacteria (PGPR) treatment yielded minimum hulling efficiency and finished product recovery for all the varieties. PMID:24803699

  5. Draft genome sequence of pigeonpea (Cajanus cajan), an orphan legume crop of resource-poor farmers

    DEFF Research Database (Denmark)

    Varshney, Rajeev K.; Chen, Wenbin; Li, Yupeng;

    2012-01-01

    Pigeonpea is an important legume food crop grown primarily by smallholder farmers in many semi-arid tropical regions of the world. We used the Illumina next-generation sequencing platform to generate 237.2 Gb of sequence, which along with Sanger-based bacterial artificial chromosome end sequences...

  6. Radiation studies in Cajanus cajan: meiotic behaviour in some M/sub 2/ mutants

    Energy Technology Data Exchange (ETDEWEB)

    Sinha, S.S.N.; Akhaury, S.B. (Ranchi Univ. (India). Dept. of Botany)

    1982-01-01

    A qualitative study of the mutants produced in M/sub 2/ generation has been made. The mutants were classified as: (1) chlorophyll mutant, (2) morphological mutant, (3) pollen mutant, (4) semi-sterile and (5) sterile mutant. Cytological investigations of pollen mutants, sterile and semi-sterile mutants have revealed that these mutants generally arise at higher dose levels (20 Kr and 25 Kr).

  7. A New Cajanus cajan Cultivar'CAF2'%木豆新品种'CAF2'

    Institute of Scientific and Technical Information of China (English)

    马宏; 李正红; 刘秀贤; 谷勇; 万友名; 李立; 梁宁

    2010-01-01

    'CAF2'是从引种自印度的木豆品种'ICPH3432'目的后代群体中,运用单株选择和集团选择育种手段选育出的木豆新品种.生育期197~202d,平均单株荚数(597.8±292.9) 个,每荚粒数(4.4 4±0.4) 粒,种子百粒质量(12.50±1.04) g,单株粒质量(205.61±121.76) g.

  8. Effect of gamma irradiation on physicochemical properties of stored pigeon pea (Cajanus cajan) flour.

    Science.gov (United States)

    Bamidele, Oluwaseun P; Akanbi, Charles T

    2013-09-01

    The effect of gamma irradiation at various doses (5, 10, 15, 20 kGy) was observed on pigeon pea flour stored for 3 months on proximate composition, functional properties, and peroxide value. Sensory evaluation was also carried out on bean cake (moinmoin) made from nonirradiated and irradiated pigeon pea flour. The results showed that stored gamma-irradiated samples had significantly lower (P < 0.05) value of protein and little or no effect on moisture content. There were slight decreases in crude fiber and ash content of the irradiated samples compared with the control sample. The result of functional properties of the irradiated flours showed slight increase in water absorption capacity, swelling capacity and bulk density. The peroxide value of crude oil increased significantly with dose increases for the period of storage. The sensory evaluation of moinmoin samples prepared from irradiated pigeon pea flour showed no significant difference from the moinmoin sample prepared from nonirradiated flour. It can be concluded that gamma irradiation can extend the shelf life of pigeon pea flour. PMID:24804044

  9. The Potential of Pigeonpea (Cajanus cajan) for Producing Important Components of Renewable Energy and Agricultural Products

    Science.gov (United States)

    Gwata, E.

    2012-04-01

    In agricultural systems, sustainable crop production is critical in meeting both environmental requirements and the limitations of drought imposed by the effects of global warming. The inputs for crop production and end use of the products should determine the choice of a crop particularly in environments prone to droughts. The objective of this paper is to highlight why a multi-purpose grain legume such as pigeonpea is an ideal crop that can be utilized for producing renewable energy. Firstly, it is highly tolerant to drought and does not require additional soil moisture after the seedling growth stage. The deep tape root extracts moisture and nutrients from deep layers of the soil concomitantly allowing for efficient nutrient recycling. The piscidic acid which is exuded from the roots enhances the solubilization of phosphorus in order to make it available for plant uptake. Secondly, the grain of pigeonpea is suitable for both human food and feedstocks. The grain is rich in oil, vitamins, minerals and protein. The grain can also be used for producing biofuel. In many countries particularly in the developing world, the stover is used as fuel wood or building (roofing) material, thus alleviating pressure on forest products. The crop is grown without the application of inorganic fertilizers as it can fix atmospheric nitrogen symbiotically in its root nodules. Pigeonpea is also ratoonable, producing two or more harvests per season. In addition, it is grown in mixed cropping systems thus optimizing land use. In these regards, pigeonpea is sustainable and environmentally friendly choice for agricultural production of food and energy balance.

  10. 木豆新品种'CAF3'%A New Cajanus cajan Cultivar 'CAF3'

    Institute of Scientific and Technical Information of China (English)

    马宏; 李正红; 刘秀贤; 谷勇; 万友名; 李立; 梁宁

    2010-01-01

    'CAF3'是运用单株选择和集团选择育种手段,从引种自印度的木豆品种'ICPH3432'F1的后代群体中选育出的木豆新品种.生育期159~162 d,平均单株荚数(470.1±177.1)个,每荚粒数(3.5±0.3)粒,种子百粒质量(8.99±0.53)g,单株粒质量(104.17±25.65)g.

  11. Genomics-assisted breeding for boosting crop improvement in pigeonpea (Cajanus cajan)

    OpenAIRE

    Pazhamala, Lekha; Saxena, Rachit K.; Singh, Vikas K.; Sameerkumar, C. V.; Kumar, Vinay; Sinha, Pallavi; Patel, Kishan; Obala, Jimmy; Kaoneka, Seleman R.; Tongoona, P.; Shimelis, Hussein A.; Gangarao, N. V. P. R.; Odeny, Damaris; Rathore, Abhishek; Dharmaraj, P. S.

    2015-01-01

    Pigeonpea is an important pulse crop grown predominantly in the tropical and sub-tropical regions of the world. Although pigeonpea growing area has considerably increased, yield has remained stagnant for the last six decades mainly due to the exposure of the crop to various biotic and abiotic constraints. In addition, low level of genetic variability and limited genomic resources have been serious impediments to pigeonpea crop improvement through modern breeding approaches. In recent years, h...

  12. 木豆良种'ICP12746'%An Elite Cajanus cajan Variety 'ICP12746'

    Institute of Scientific and Technical Information of China (English)

    马宏; 李正红; 刘秀贤; 万友名; 谷勇

    2013-01-01

    ‘ICP12746’为印度国际半干旱热带作物研究所(ICRISAT)选育的纯系品种,于1998年引进中国.生育期231 ~242天,2年生植株平均株高(206.3±21.1)cm,地径(2.48±0.37)cm,一级分枝数(21.3±2.4)枝,单株荚数(416.8±59.6)个,种子百粒质量(24.91±0.45)g,单株粒质量(270.76±43.80)g;与对照品种比较,具有产量高、豆荚及豆粒大、鲜籽粒口感好且种皮薄等优点.

  13. Genetics of Fusarium Wilt Resistance in Pigeonpea (Cajanus cajan) and Efficacy of Associated SSR Markers.

    Science.gov (United States)

    Singh, Deepu; Sinha, B; Rai, V P; Singh, M N; Singh, D K; Kumar, R; Singh, A K

    2016-04-01

    Inheritance of resistance to Fusarium wilt (FW) disease caused by Fusarium udum was investigated in pigeonpea using four different long duration FW resistant genotypes viz., BDN-2004-1, BDN-2001-9, BWR-133 and IPA-234. Based on the F2 segregation pattern, FW resistance has been reported to be governed by one dominant gene in BDN-2004-1 and BDN-2001-9, two duplicate dominant genes in BWR-133 and two dominant complimentary genes in resistance source IPA-234. Further, the efficacy of six simple sequence repeat (SSR) markers namely, ASSR-1, ASSR-23, ASSR-148, ASSR-229, ASSR-363 and ASSR-366 reported to be associated with FW resistance were also tested and concluded that markers ASSR-1, ASSR-23, ASSR-148 will be used for screening of parental genotypes in pigeonpea FW resistance breeding programs. The information on genetics of FW resistance generated from this study would be used, to introgress FW resistance into susceptible but highly adopted cultivars through marker-assisted backcross breeding and in conventional breeding programs. PMID:27147929

  14. Genomics-assisted breeding for boosting crop improvement in pigeonpea (Cajanus cajan).

    Science.gov (United States)

    Pazhamala, Lekha; Saxena, Rachit K; Singh, Vikas K; Sameerkumar, C V; Kumar, Vinay; Sinha, Pallavi; Patel, Kishan; Obala, Jimmy; Kaoneka, Seleman R; Tongoona, P; Shimelis, Hussein A; Gangarao, N V P R; Odeny, Damaris; Rathore, Abhishek; Dharmaraj, P S; Yamini, K N; Varshney, Rajeev K

    2015-01-01

    Pigeonpea is an important pulse crop grown predominantly in the tropical and sub-tropical regions of the world. Although pigeonpea growing area has considerably increased, yield has remained stagnant for the last six decades mainly due to the exposure of the crop to various biotic and abiotic constraints. In addition, low level of genetic variability and limited genomic resources have been serious impediments to pigeonpea crop improvement through modern breeding approaches. In recent years, however, due to the availability of next generation sequencing and high-throughput genotyping technologies, the scenario has changed tremendously. The reduced sequencing costs resulting in the decoding of the pigeonpea genome has led to the development of various genomic resources including molecular markers, transcript sequences and comprehensive genetic maps. Mapping of some important traits including resistance to Fusarium wilt and sterility mosaic disease, fertility restoration, determinacy with other agronomically important traits have paved the way for applying genomics-assisted breeding (GAB) through marker assisted selection as well as genomic selection (GS). This would accelerate the development and improvement of both varieties and hybrids in pigeonpea. Particularly for hybrid breeding programme, mitochondrial genomes of cytoplasmic male sterile (CMS) lines, maintainers and hybrids have been sequenced to identify genes responsible for cytoplasmic male sterility. Furthermore, several diagnostic molecular markers have been developed to assess the purity of commercial hybrids. In summary, pigeonpea has become a genomic resources-rich crop and efforts have already been initiated to integrate these resources in pigeonpea breeding. PMID:25741349

  15. Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp].

    Science.gov (United States)

    Júnior, Paulo Ivan Fernandes; de Lima, Andréa Aparecida; Passos, Samuel Ribeiro; Tuão Gava, Carlos Alberto; de Oliveira, Paulo Jansen; Rumjanek, Norma Gouvêa; Xavier, Gustavo Ribeiro

    2012-10-01

    This study evaluated 26 pigeonpea rhizobial isolates according to their cultural characteristics, intrinsic antibiotic resistance, salt and temperature tolerance, carbon source utilization and amylolytic activity. The cultural characterization showed that the majority of them presented the ability to acidify the YMA. Among the 27 isolates evaluated, 25 were able to grow when incubated at 42° C and 11 showed tolerance to 3% (w/v) of NaCl in YMA medium. The patterns of carbon sources utilization was very diverse among the isolates. It was observed the capacity of three strains to metabolize all the carbon sources evaluated and a total of 42% of the bacterial isolates was able to grow in the culture medium supplemented with at least, six carbon sources. The carbon sources mannitol (control) and sucrose were metabilized by all isolates evaluated. The profile of intrinsic resistance to antibiotics showed that the isolates were mostly resistant to streptomycin and ampicillin, but susceptible to kanamycin and chloranphenicol. High amylolytic activity of, at least, four isolates was also demonstrated, especially for isolated 47.3b, which showed the highest enzymatic index. These results indicate the metabolic versatility of the pigeonpea rhizobia, and indicates the isolate 47.3b to further studies regarding the amylase production and characterization. PMID:24031992

  16. Genome wide in silico characterization of Dof gene families of pigeonpea (Cajanus cajan (L) Millsp.).

    Science.gov (United States)

    Malviya, N; Gupta, S; Singh, V K; Yadav, M K; Bisht, N C; Sarangi, B K; Yadav, D

    2015-02-01

    The DNA binding with One Finger (Dof) protein is a plant specific transcription factor involved in the regulation of wide range of processes. The analysis of whole genome sequence of pigeonpea has identified 38 putative Dof genes (CcDof) distributed on 8 chromosomes. A total of 17 out of 38 CcDof genes were found to be intronless. A comprehensive in silico characterization of CcDof gene family including the gene structure, chromosome location, protein motif, phylogeny, gene duplication and functional divergence has been attempted. The phylogenetic analysis resulted in 3 major clusters with closely related members in phylogenetic tree revealed common motif distribution. The in silico cis-regulatory element analysis revealed functional diversity with predominance of light responsive and stress responsive elements indicating the possibility of these CcDof genes to be associated with photoperiodic control and biotic and abiotic stress. The duplication pattern showed that tandem duplication is predominant over segmental duplication events. The comparative phylogenetic analysis of these Dof proteins along with 78 soybean, 36 Arabidopsis and 30 rice Dof proteins revealed 7 major clusters. Several groups of orthologs and paralogs were identified based on phylogenetic tree constructed. Our study provides useful information for functional characterization of CcDof genes. PMID:25344821

  17. Cultivar preference and sensory evaluation of vegetable pigeon pea (Cajanus cajan) in Eastern Kenya

    Science.gov (United States)

    Preference and acceptability of twelve vegetable pigeon pea genotypes of medium maturity was evaluated in Eastern Kenya based on six seed cultivar parameters of color, appearance, taste, odor, tenderness and overall seed acceptability. The sensory characteristics were scored by consumers and farmers...

  18. Utilization of Decorticated Pigeon Pea (Cajanus cajan L. With Wheat (Triticum aestivum Flours in Bread Making

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    H.A. Hassan

    2011-12-01

    Full Text Available The objectives of this study were to investigate the use of decorticated pigeon pea flour in the development of protein rich - bread, suitable for general and specific nutritional purposes and to study the effect of incorporation of pigeon pea flour on the sensory evaluation and quality of bread produced. Decorticated Pigeon Pea Flour (DPPF was incorporated with wheat flour (WF 72% Ext. to replace 0, 5, 10, 15, 20 and 25% of the wheat flour for bread making. Proximate composition, falling number, gluten quality and quantity, tannins and phytic acid were determined for the flour blends (Composite flour. Bread proximate composition, sensory evaluation and specific volume were determined as well. Decortication of pigeon pea led to decrease in moisture, ash, tannins and phytic acid and increase in the protein and carbohydrates contents. The falling number (alpha amylase activity significantly increased over the control with the increasing level of DPPF. There were also significant reduction (p#0.05 on gluten quantity (wet and dry gluten and quality (gluten index. No significant differences were found in bread specific volume up to 10% addition of DPPF. The protein, ash, fat contents and calorific values for the bread were significantly increased (p#0.05 with incorporation of DPPF. Increasing levels of the replacement of DPPF resulted in a decrease in the organoleptic quality of the bread. The bread containing up to 15% DPPF was found to be the best in overall acceptability.

  19. Estimation of Heterosis, Combining ability and Gene action in Pigeonpea [Cajanus cajan (L. Millsp.

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    Yamanura,, *Lokesha R., Dharmaraj, P.S., Muniswamy, S. and Diwan, J.R.

    2014-06-01

    Full Text Available Eight cytoplasmic-genetic male sterile lines with A2 (Cajanus scarabaeoides and A4 (Cajanus cajanifolius cytoplasm were crossed with 10 testers in a line × tester design. Resultant 80 experimental hybrids were evaluated along with parents and check (Maruti in lattice design with two replications. Combining ability analysis evinced predominance of non-additive gene effects for 10 characters indicating relevance of heterosis breeding for improving yield attributes. The gca effects of parents revealed that ICPA-2078, GT-308A, PKV-TATA, ARCCV-2 and GPHR-08-11 were good general combiners for seed yield and its direct components. The estimates of sca effects revealed that nine experimental hybrids had significant, desirable and positive sca effects for seed yield. The cross combination ICPA-2092 x VIPUL, ICPA-2078 x BSMR-856 and ICPA-2078 x ARCCV-2 were good specific combiners for number of secondary branches, number of pods per plant, seed yield per plant. These parental combinations are being used for exploitation of hybrid vigour.

  20. Morphological characterization and assessment of genetic diversity in minicore collection of pigeonpea [Cajanus Cajan (L. Millsp.

    Directory of Open Access Journals (Sweden)

    Muniswamy, S., Lokesha, R. *, Dharmaraj, P.S., Yamanura1 and Diwan, J.R.

    2014-06-01

    Full Text Available An investigation was undertaken to ascertain the extent of genetic diversity present among 196 pigeon pea genotypes using D2 statistic. A wider genetic diversity was observed for nine characters as evidenced by formation of 13 clusters. Number of pods per plant contributed most (59.83% towards divergence, followed by plant height ( 21.55 The highest inter cluster distance was observed between the cluster XIII and VII, followed by cluster V and XIII, II and XIII and cluster XII and VII, which indicates that the crosses among the genotypes between these clusters may result in better segregants and high heterotic combinations. Cluster mean analysis indicated that cluster V contains dwarf and early maturing genotypes and cluster XIII possess high yielding entries. Morphological characterization was also carried out for 15 traits can be used in varietal purification and seed production.

  1. Facile combustion synthesis of ZnO nanoparticles using Cajanus cajan (L.) and its multidisciplinary applications

    Energy Technology Data Exchange (ETDEWEB)

    Manjunath, K.; Ravishankar, T.N. [Centre for Nano and Material Sciences, Jain University, Jakkasandra, Kanakapura Talluk (India); Kumar, Dhanith [Department of Chemistry, B.M.S. Instsitute of Technology, Yelahanka, Bangalore (India); Priyanka, K.P; Varghese, Thomas [Nanoscience Research Centre, Department of Physics, Nirmala College, Muvattupuzha, Kerala (India); Naika, H.Raja [Department of Studies and Research in Environmental Science, Tumkur University, Tumkur (India); Nagabhushana, H. [CNR Rao Center for Advanced Materials, Tumkur University, Tumkur (India); Sharma, S.C. [Chattisgarh Swami Vivekananda Technical University, Bhilai (India); Dupont, J. [Institute of Chemistry, Laboratory of Molecular Catalysis, UFRGS, Porto Alegre (Brazil); Ramakrishnappa, T. [Centre for Nano and Material Sciences, Jain University, Jakkasandra, Kanakapura Talluk (India); Nagaraju, G., E-mail: nagarajugn@rediffmail.com [Department of Chemistry, B.M.S. Instsitute of Technology, Yelahanka, Bangalore (India)

    2014-09-15

    Graphical abstract: Facile combustion synthesis of ZnO nanoparticles using Cajanuscajan (L.) and its multidisciplinary applications.Zinc oxide nanoparticles were successfully synthesized by solution combustion method (SCM) using pigeon pea as a combustible fuel for the first time. The as-prepared product shows good photocatalytic, dielectric, antibacterial, electrochemical properties. - Highlights: • ZnO Nps were synthesized via combustion method using pigeon pea as a fuel. • The structure of the product was confirmed by XRD technique. • The morphology was confirmed by SEM and TEM images. • The as-prepared product shown good photocatalytic activity, dielectric property. • It has also shown good antibacterial and electrochemical properties. - Abstract: Zinc oxide nanoparticles (ZnO Nps) were successfully synthesized by solution combustion method (SCM) using pigeon pea as a fuel for the first time. X-Ray diffraction pattern reveals that the product belongs to hexagonal system. FTIR spectrum of ZnO Nps shows the band at 420 cm{sup −1} associated with the characteristic vibration of Zn–O. TEM images show that the nanoparticles are found to be ∼40–80 nm. Furthermore, the as-prepared ZnO Nps exhibits good photocatalytic activity for the photodegradation of methylene blue (MB), indicating that they are indeed a promising photocatalytic semiconductor. The antibacterial properties of ZnO nanopowders were investigated by their bactericidal activity against four bacterial strains.

  2. Genomics-assisted breeding for boosting crop improvement in pigeonpea (Cajanus cajan

    Directory of Open Access Journals (Sweden)

    Lekha ePazhamala

    2015-02-01

    Full Text Available Pigeonpea is an important pulse crop grown predominantly in the tropical and sub-tropical regions of the world. Although pigeonpea growing area has considerably increased, yield has remained stagnant for the last six decades mainly due to the exposure of the crop to various biotic and abiotic constraints. In addition, low level of genetic variability and limited genomic resources have been serious impediments to pigeonpea crop improvement through modern breeding approaches. In recent years, however, due to the availability of next generation sequencing and high-throughput genotyping technologies, the scenario has changed tremendously. The reduced sequencing costs resulting in the decoding of the pigeonpea genome has led to the development of various genomic resources including molecular markers, transcript sequences and comprehensive genetic maps. Mapping of some important traits including resistance to Fusarium wilt and sterility mosaic disease, fertility restoration, determinacy with other agronomically important traits have paved the way for applying genomics-assisted breeding (GAB through marker assisted selection as well as genomic selection. This would lead to accelerate the development and improvement of both varieties and hybrids in pigeonpea. Particularly for hybrid breeding programme, mitochondrial genomes of cytoplasmic male sterile lines, maintainers and hybrids have also been sequenced to identify genes responsible for cytoplasmic male sterility. Furthermore, several diagnostic molecular markers have been developed to assess the purity of commercial hybrids. In summary, pigeonpea has become a genomic resources-rich crop and efforts have already been initiated to integrate these resources in pigeonpea breeding.

  3. Hydroxypropylation of pigeon pea (cajanus cajan) starch: Preparation, functional characterizations and enzymatic digestibility

    International Nuclear Information System (INIS)

    Hydroxypropyl starch derivatives were prepared from pigeon pea starch (NPPS) which is an unconventional starch source. Functional parameters and characterization of both native and modified starches were carried out. The starch granules appeared oval or elliptical in shape with sizes ranging from 7 - 40 μm in width and 10 . 30 μm in length. Hydroxypropylation did not alter the shape of the starch granules in a pronounced way. Generally, the x-ray diffractograms of both native and hydroxypropyl derivatives showed the 'C' pattern. However, slight reductions were observed in the intensity of starches after modification. At all temperatures studied (30 - 90 deg. C), swelling and solubility of hydroxypropylated starches were higher than the NPPS. Progressive increases in swelling capacity and solubility were observed as the MS increased among the hydroxypropylated starches. Hydroxypropylation reduced starch paste turbidity on storage. Also, studies showed that syneresis reduced after hydroxypropylation. In addition, syneresis reduced as the MS of the hydroxypropyl starches increased. The results indicate that pasting temperature and peak temperature reduced after modification but peak viscosity increased in hydroxypropylated starch derivatives compared with the native starch. Setback reduced in hydroxypropylated starches compared with the native starch. Enthalpy of gelatinization and percentage retrogradation reduced after hydroxypropylation and progressive reductions were observed as the MS increased among the starch derivatives. Hydroxypropylation increased enzymatic digestibility. (author)

  4. AGROBACTERIUM MEDIATED TRANSFORMATION OF PIGEONPEA (CAJANUS CAJAN L MILLLSP VAR LRG-41 FROM AXILLARY BUD

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    T. Raghavendra

    2014-02-01

    Full Text Available A reliable method of plant regeneration has been achieved from Axillary buds. Shoots appeared from explants when cultured on Murashige and skoog (MS medium supplemented with BAP (Benzyl amino purine, Napthalene acetic acid (NAA and Kinetin at various combinations. Elongated shoots were rooted with 70.6% rooting frequency in MS medium with indole buteric acid (IBA at 1.0mg/l. The rooted plantlets were established well in soilrite mixture medium with 91% success and days taken for acclimatization were 12.8 days. This protocol was used in Agrobacterium mediated transformation. The transformation was carried out using the Agrobacterium strain LBA4404 containing the binary vector pCAMBIA2301 harboring npt II as selectable marker and GUS as reporter gene.

  5. AGROBACTERIUM MEDIATED TRANSFORMATION OF PIGEONPEA (CAJANUS CAJAN L MILLLSP VAR LRG-41 FROM AXILLARY BUD

    Directory of Open Access Journals (Sweden)

    T. Raghavendra

    2014-03-01

    Full Text Available A reliable method of plant regeneration has been achieved from Axillary buds. Shoots appeared from explants when cultured on Murashige and skoog (MS medium supplemented with BAP (Benzyl amino purine, Napthalene acetic acid (NAA and Kinetin at various combinations. Elongated shoots were rooted with 70.6% rooting frequency in MS medium with indole buteric acid (IBA at 1.0mg/l. The rooted plantlets were established well in soilrite mixture medium with 91% success and days taken for acclimatization were 12.8 days. This protocol was used in Agrobacterium mediated transformation. The transformation was carried out using the Agrobacterium strain LBA4404 containing the binary vector pCAMBIA2301 harboring npt II as selectable marker and GUS as reporter gene.

  6. Deciphering Transcriptional Programming during Pod and Seed Development Using RNA-Seq in Pigeonpea (Cajanus cajan)

    Science.gov (United States)

    Pazhamala, Lekha T.; Agarwal, Gaurav; Bajaj, Prasad; Kumar, Vinay; Kulshreshtha, Akanksha; Saxena, Rachit K.; Varshney, Rajeev K.

    2016-01-01

    Seed development is an important event in plant life cycle that has interested humankind since ages, especially in crops of economic importance. Pigeonpea is an important grain legume of the semi-arid tropics, used mainly for its protein rich seeds. In order to understand the transcriptional programming during the pod and seed development, RNA-seq data was generated from embryo sac from the day of anthesis (0 DAA), seed and pod wall (5, 10, 20 and 30 DAA) of pigeonpea variety “Asha” (ICPL 87119) using Illumina HiSeq 2500. About 684 million sequencing reads have been generated from nine samples, which resulted in the identification of 27,441 expressed genes after sequence analysis. These genes have been studied for their differentially expression, co-expression, temporal and spatial gene expression. We have also used the RNA-seq data to identify important seed-specific transcription factors, biological processes and associated pathways during seed development process in pigeonpea. The comprehensive gene expression study from flowering to mature pod development in pigeonpea would be crucial in identifying candidate genes involved in seed traits directly or indirectly related to yield and quality. The dataset will serve as an important resource for gene discovery and deciphering the molecular mechanisms underlying various seed related traits. PMID:27760186

  7. Analysis of toxic elements in two pigeonpea (Cajanus cajan (L.) Millsp) cultivars, in fertilized soils, by neutron activation; Analise de elementos toxicos em duas variedades de guandu (Cajanus cajan (L.) Millsp), cultivadas em solos tratados, por ativacao com neutrons

    Energy Technology Data Exchange (ETDEWEB)

    Piasentin, Ricardo M.; Armelin, Maria Jose A. [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil). Divisao de Radioquimica; Primavesi, Odo [Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA), Sao Carlos, SP (Brazil). Centro de Pesquisa de Pecuaria do Sudeste

    2000-07-01

    Samples of whole leaves, from thirty-six plants belonging to two pigeonpea cultivars were analysed by Instrumental Neutron Activation Analysis. Each plant was cultivated under either single dose or double one of some mineral fertilizers, such as, B, Co, Cu, Fe, Mn, Mo, V and Zn, individually, to the soil; besides limestone and phosphorus. The aim of this paper is to evaluate the contribution of these treatments to the increase in the concentrations of As, Sb, Th and U, since these elements can be toxic to plants and animals. (author)

  8. Avaliação agronômica de linhagens selecionadas de guandu (Cajanus cajan (L. Millsp Agronomic evaluation of selected pigeon-pea lines (Cajanus cajan (L. Millsp

    Directory of Open Access Journals (Sweden)

    Rodolfo Godoy

    2005-02-01

    Full Text Available Com o objetivo de reavaliar agronomicamente 17 linhagens de guandu, originárias de acessos anteriormente selecionados por diversas características de interesse agronômico, foram instalados experimentos em cinco locais do Estado de São Paulo. A confirmação dessas características foi necessária, uma vez que as amostras de sementes dos acessos originais apresentavam mistura mecânica e segregação, razão pela qual, após serem selecionados, os acessos passaram por processo de purificação em condições controladas de polinização. Nesses experimentos, as linhagens foram submetidas a cortes para avaliação da produção de forragem, ocasião em que foram determinados o número de plantas na área útil da parcela e a altura média dessas plantas. Foi também determinado o teor de proteína bruta e de taninos de amostras da forragem produzida. Entre as linhagens testadas, g58-95 e g127-97 confirmaram ter plantas de baixa estatura, g3-94, g167-97 e g29b-94 confirmaram oferecer boas produções de forragem e g146-97 destacou-se quanto à produção inicial de matéria seca.With the purpose of re-evaluating seventeen pigeon-pea lines originated from accessions previously selected for favorable agronomic characteristics, experiments were installed in five locations of the State of São Paulo. The confirmation of those characteristics was necessary because the original accessions presented various degrees of mechanical mixtures and segregation and were submitted to purification process in pollination controlled conditions. In those trials, the lines were cut in several occasions to evaluate forage yield. Each time, it was determined the number of plants in each parcel and its average height. Also it was determined their crude protein and tannins contents. Among the tested lines, g58-95 e g127-97 confirmed to have low plants, g94, g167-97 and g29b-94, confirmed to offer good forage yields, while line g146-97 presented good initial forage yields.

  9. Molecular cloning and characterization of the light-harvesting chlorophyll a/b gene from the pigeon pea (Cajanus cajan).

    Science.gov (United States)

    Qiao, Guang; Wen, Xiao-Peng; Zhang, Ting

    2015-12-01

    Light-harvesting chlorophyll a/b-binding proteins (LHCB) have been implicated in the stress response. In this study, a gene encoding LHCB in the pigeon pea was cloned and characterized. Based on the sequence of a previously obtained 327 bp Est, a full-length 793 bp cDNA was cloned using the rapid amplification of cDNA ends (RACE) method. It was designated CcLHCB1 and encoded a 262 amino acid protein. The calculated molecular weight of the CcLHCB1 protein was 27.89 kDa, and the theoretical isoelectric point was 5.29. Homology search and sequence multi-alignment demonstrated that the CcLHCB1 protein sequence shared a high identity with LHCB from other plants. Bioinformatics analysis revealed that CcLHCB1 was a hydrophobic protein with three transmembrane domains. By fluorescent quantitative real-time polymerase chain reaction (PCR), CcLHCB1 mRNA transcripts were detectable in different tissues (leaf, stem, and root), with the highest level found in the leaf. The expression of CcLHCB1 mRNA in the leaves was up-regulated by drought stimulation and AM inoculation. Our results provide the basis for a better understanding of the molecular organization of LCHB and might be useful for understanding the interaction between plants and microbes in the future. PMID:26329890

  10. A comparative method for protein extraction and 2-D gel electrophoresis from different tissues of Cajanus cajan.

    Science.gov (United States)

    Singh, Nisha; Jain, Neha; Kumar, Ram; Jain, Ajay; Singh, Nagendra K; Rai, Vandna

    2015-01-01

    Pigeonpea is an important legume crop with high protein content. However, it is often subjected to various abiotic and biotic stresses. Proteomics is a state-of-the-art technique used to analyze the protein profiling of a tissue for deciphering the molecular entities that could be manipulated for developing crops resistant to these stresses. In this context, developing a comprehensive proteome profile from different vegetative and reproductive tissues has become mandatory. Although several protein extraction protocols from different tissues of diverse plant species have been reported, there is no report for pigeonpea. Here, we report tissue-specific protein extraction protocols representing vegetative (young leaves), and reproductive (flowers and seeds) organs and their subsequent analysis on 2-dimensional gel electrophoresis. The study explicitly demonstrated that the efficacy of a particular protein extraction protocol is dependent on the different tissues, such as leaves, flowers and seeds that differ in their structure and metabolic constituents. For instance, phenol-based protocol showed an efficacy toward higher protein yield, better spot resolution and a minimal streaking on 2-DE gel for both leaves and flowers. Protein extraction from seeds was best achieved by employing phosphate-TCA-acetone protocol. PMID:26300903

  11. 木豆叶的化学成分研究%Chemical constituents from leaves of Cajanus cajan

    Institute of Scientific and Technical Information of China (English)

    刘亚旻; 姜保平; 沈胜楠; 郭智; 李宗阳; 斯建勇; 潘瑞乐

    2014-01-01

    目的 研究木豆Cajanus cajan叶的化学成分.方法 通过正相硅胶色谱、中压反相硅胶色谱(ODS)、反相大孔树脂(MCI)、Sephadex LH-20凝胶等多种技术进行分离纯化,并通过核磁共振谱、质谱等波谱数据鉴定其结构.结果 从木豆叶醇提物中分离得到23个化合物,分别鉴定为木豆素(1)、木豆素A(2)、木豆素C(3)、木豆内酯A(4)、球松素(5)、荭草苷(6)、异牡荆苷(7)、牡荆苷(8)、cajanol (9)、cajanin (10)、樱黄素(11)、红车轴草素(12)、(2R,3R)-2,3-二氢-5-7,4 '-二甲氧基黄酮(13)、10′,16′-二羟基棕榈酸乙酯(14)、香草酸(15)、十七烷酸乙酯(16)、2-O-甲基肌醇(17)、2,3,4-三羟基异戊酸(18)、豆甾醇(19)、桦木酸(20)、十七烷酸(21)、β-谷甾醇(22)和β-胡萝卜苷(23).结论 化合物10~19均为首次从该属植物中分离得到.

  12. Using AFLP-RGA markers to assess genetic diversity among pigeon pea (Cajanus cajan genotypes in relation to major diseases

    Directory of Open Access Journals (Sweden)

    Prakash G Pati

    2014-06-01

    Full Text Available Resistance gene analog (RGA-anchored amplified fragment length polymorphism (AFLP-RGA marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses to Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S, M-GTG/wlrk-S, M-GTG/wlrk-AS, E-CAT/S1-INV and E-CAG/wlrk-AS produced 173 scorable fragments, of which 157 (90.7% were polymorphic, with an average of 31.4 fragments per primer combination. The polymorphism rates obtained with the five primers were 83.3%, 92.0%, 92.3%, 93.0% and 93.1%, respectively. Mean polymorphic information content (PIC values ranged from 0.24 (with E-CAT/S1-INV to 0.30 (with E-CAG/wlrk-AS, whereas resolving power (RP values varied from 11.06 (with M-GTG/wlrk-S to 25.51 (with E-CAG/wlrk-AS and marker index (MI values ranged from 5.98 (with M-GTG/wlrk-S to 12.30 (with E-CAG/wlrk-AS. We identified a positive correlation between MI and RP (r²=0.98, p<0.05, stronger that that observed for the comparison between PIC and RP (r²=0.88, p<0.05. That implies that either MI or RP is the best parameter for selecting more informative AFLP-RGA primer combinations. The Jaccard coefficient ranged from 0.07 to 0.72, suggesting a broad genetic base in the genotypes studied. A neighbor-joining tree, based on the unweighted pair group method with arithmetic mean, distinguished cultivated species from wild species. The grouping of resistant genotypes in different clusters would help in the selection of suitable donors for resistance breeding in pigeon pea.

  13. Quality evaluation of stiff porridges prepared from Irish potato (Solanum tuberosum) and pigeon pea (Cajanus cajan) starch blends.

    Science.gov (United States)

    Abu, Joseph Oneh; Enyinnaya, Chinma Chiemela; James, Samaila; Okeleke, Ezinne

    2012-06-01

    Quality attributes of stiff porridges prepared from Irish potato and pigeon pea starch blends were studied. Starches were extracted from Irish potato and pigeon pea using a wet extraction method. Various ratios of the starches were mixed and analyzed for chemical, functional and pasting properties. The starch blends were then prepared into stiff porridges for sensory evaluation using a 20-man sensory panel. Substitution of Irish potato starch with pigeon pea starch led to increases in protein (0.15 to 1.2%), fat (0.26 to 0.56%) and ash (0.30 to 0.69%) while the amylose content of the starch blends decreased (from 23.8 to 18.4%) respectively. Functional properties such as bulk density (0.75 to 0.60 g/cm(3)), water absorption capacity (3.1 to 2.6 g water/ g sample) and dispersibility (58.6 to 42.7%) decreased significantly (P < 0.05) at the highest concentration (50%) of pigeon pea starch respectively. Pasting properties such as peak, breakdown, final and setback viscosities increased with increasing levels of pigeon pea starch while peak time and pasting temperature decreased. The sensory attributes of stiff porridges were not adversely affected by pigeon pea starch inclusion. Therefore it should be possible to incorporate up to 50% of low digestible pigeon pea starch into Irish potato starch from legumes such as pigeon pea as alternatives to cassava starch in the preparation of stiff porridges. Such porridges made from Irish potato and legume starches could provide additional incentive for individuals requiring decreased and or slow starch digestibility such as diabetics. PMID:23729855

  14. Genetic diversity studies and identification of SSR markers associated with Fusarium wilt (Fusarium udum) resistance in cultivated pigeonpea (Cajanus cajan).

    Science.gov (United States)

    Singh, A K; Rai, V P; Chand, R; Singh, R P; Singh, M N

    2013-01-01

    Genetic diversity and identification of simple sequence repeat markers correlated with Fusarium wilt resistance was performed in a set of 36 elite cultivated pigeonpea genotypes differing in levels of resistance to Fusarium wilt. Twenty-four polymorphic sequence repeat markers were screened across these genotypes, and amplified a total of 59 alleles with an average high polymorphic information content value of 0.52. Cluster analysis, done by UPGMA and PCA, grouped the 36 pigeonpea genotypes into two main clusters according to their Fusarium wilt reaction. Based on the Kruskal-Wallis ANOVA and simple regression analysis, six simple sequence repeat markers were found to be significantly associated with Fusarium wilt resistance. The phenotypic variation explained by these markers ranged from 23.7 to 56.4%. The present study helps in finding out feasibility of prescreened SSR markers to be used in genetic diversity analysis and their potential association with disease resistance. PMID:23970083

  15. UV-induced changes of active components and antioxidant activity in postharvest pigeon pea [Cajanus cajan (L.) Millsp.] leaves.

    Science.gov (United States)

    Wei, Zuo-Fu; Luo, Meng; Zhao, Chun-Jian; Li, Chun-Ying; Gu, Cheng-Bo; Wang, Wei; Zu, Yuan-Gang; Efferth, Thomas; Fu, Yu-Jie

    2013-02-13

    In this study, the effect of UV irradiation (UV-A, UV-B, and UV-C) on phytochemicals, total phenolics, and antioxidant activity of postharvest pigeon pea leaves was evaluated. The response of pigeon pea leaves to UV irradiation was phytochemical specific. UV-B and UV-C induced higher levels of phytochemicals, total phenolics, and antioxidant activity in pigeon pea leaves compared with UV-A. Furthermore, UV-B irradiation proved to possess a long-lasting effect on the levels of phenolics and antioxidant activity. After adapting for 48 h at 4 °C following 4 h UV-B irradiation, total phenolics and antioxidant activity were approximately 1.5-fold and 2.2-fold increased from 39.4 mg GAE/g DM and 15.0 μmol GAE/g DM to 59.1 mg GAE/g DM and 32.5 μmol GAE/g DM, respectively. These results indicate that UV irradiation of pigeon pea leaves can be beneficial in terms of increasing active components and antioxidant activity. PMID:23320913

  16. Effect of pigeon pea (Cajanus cajan L.) on high-fat diet-induced hypercholesterolemia in hamsters.

    Science.gov (United States)

    Dai, Fan-Jhen; Hsu, Wei-Hsuan; Huang, Jan-Jeng; Wu, She-Ching

    2013-03-01

    Obesity is associated with increased systemic and airway oxidative stress, which may result from a combination of adipokine imbalance and antioxidant defenses reduction. Obesity-mediated oxidative stress plays an important role in the pathogenesis of dyslipidemia, vascular disease, and nonalcoholic hepatic steatosis. The antidyslipidemic activity of pigeon pea were evaluated by high-fat diet (HFD) hamsters model, in which the level of high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), total cholesterol (TC), and total triglyceride (TG) were examined. We found that pigeon pea administration promoted cholesterol converting to bile acid in HFD-induced hamsters, thereby exerting hypolipidemic activity. In the statistical results, pigeon pea significantly increased hepatic carnitine palmitoyltransferase-1 (CPT-1), LDL receptor, and cholesterol 7α-hydroxylase (also known as cytochrome P450 7A1, CYP7A1) expression to attenuate dyslipidemia in HFD-fed hamsters; and markedly elevated antioxidant enzymes in the liver of HFD-induced hamsters, further alleviating lipid peroxidation. These effects may attribute to pigeon pea contained large of unsaturated fatty acids (UFA; C18:2) and phytosterol (β-sitosterol, campesterol, and stigmasterol). Moreover, the effects of pigeon pea on dyslipidemia were greater than β-sitosterol administration (4%), suggesting that phytosterone in pigeon pea could prevent metabolic syndrome. PMID:23287313

  17. 早熟木豆新品种'CAF4'%A New Early Ripening Cajanus cajan Cultivar 'CAF4'

    Institute of Scientific and Technical Information of China (English)

    马宏; 李正红; 刘秀贤; 谷勇; 万友名; 李立; 梁宁

    2010-01-01

    'CAF4'是运用单株选择和集团选择育种手段,从引种自印度的木豆品种'ICPH3432'F1后代群体中选育出的木豆新品种.生育期162~166d,平均单株荚数(644.4±202.4)个,每荚粒数(3.6 ±0.4)粒,种子百粒质量(9.62 ±0.89)g,单株粒质量(113.76 ±41.64)g.

  18. Delayed flowering is associated with lack of photosynthetic acclimation in Pigeon pea (Cajanus cajan L.) grown under elevated CO₂.

    Science.gov (United States)

    Sreeharsha, Rachapudi Venkata; Sekhar, Kalva Madhana; Reddy, Attipalli Ramachandra

    2015-02-01

    In the present study, we investigated the likely consequences of future atmospheric CO2 concentrations [CO2] on growth, physiology and reproductive phenology of Pigeonpea. A short duration Pigeonpea cultivar (ICPL 15011) was grown without N fertilizer from emergence to final harvest in CO2 enriched atmosphere (open top chambers; 550μmolmol(-1)) for two seasons. CO2 enrichment improved both net photosynthetic rates (Asat) and foliar carbohydrate content by 36 and 43%, respectively, which further reflected in dry biomass after harvest, showing an increment of 29% over the control plants. Greater carboxylation rates of Rubisco (Vcmax) and photosynthetic electron transport rates (Jmax) in elevated CO2 grown plants measured during different growth periods, clearly demonstrated lack of photosynthetic acclimation. Further, chlorophyll a fluorescence measurements as indicated by Fv/Fm and ΔF/Fm' ratios justified enhanced photosystem II efficiency. Mass and number of root nodules were significantly high in elevated CO2 grown plants showing 58% increase in nodule mass ratio (NMR) which directly correlated with Pn. Growth under high CO2 showed significant ontogenic changes including delayed flowering. In conclusion, our data demonstrate that the lack of photosynthetic acclimation and increased carbohydrate-nitrogen reserves modulate the vegetative and reproductive growth patterns in Pigeonpea grown under elevated CO2. PMID:25575994

  19. Nitrate reductase and nitrite as additional components of defense system in pigeonpea (Cajanus cajan L.) against Helicoverpa armigera herbivory.

    Science.gov (United States)

    Kaur, Rimaljeet; Gupta, Anil Kumar; Taggar, Gaurav Kumar

    2014-10-01

    Amylase inhibitors serve as attractive candidates of defense mechanisms against insect attack. Therefore, the impediment of Helicoverpa armigera digestion can be the effective way of controlling this pest population. Nitrite was found to be a potent mixed non-competitive competitive inhibitor of partially purified α-amylase of H. armigera gut. This observation impelled us to determine the response of nitrite and nitrate reductase (NR) towards H. armigera infestation in nine pigeonpea genotypes (four moderately resistant, three intermediate and two moderately susceptible). The significant upregulation of NR in moderately resistant genotypes after pod borer infestation suggested NR as one of the factors that determine their resistance status against insect attack. The pod borer attack caused greater reduction of nitrate and significant accumulation of nitrite in moderately resistant genotypes. The activity of nitrite reductase (NiR) was also enhanced more in moderately resistant genotypes than moderately susceptible genotypes on account of H. armigera herbivory. Expression of resistance to H. armigera was further revealed when significant negative association between NR, NiR, nitrite and percent pod damage was observed. This is the first report that suggests nitrite to be a potent inhibitor of H. armigera α-amylase and also the involvement of nitrite and NR in providing resistance against H. armigera herbivory. PMID:25307464

  20. Development of a potential functional food prepared with pigeon pea (Cajanus cajan), oats and Lactobacillus reuteri ATCC 55730.

    Science.gov (United States)

    Barboza, Yasmina; Márquez, Enrique; Parra, Katynna; Piñero, M Patricia; Medina, Luis M

    2012-11-01

    The purpose of this study was to investigate the survival of Lactobacillus reuteri ATCC 55730 in creams, prepared with pigeon peas and oat. Products were analysed to determine their content of protein, fibre, fat, carbohydrates and degree of likeness. Viable numbers of L. reuteri and pH were determined after 1, 7, 14, 21 and 28 days of storage at 4°C. Results showed significant differences (P 0.05) were found on sensory quality between control and creams with L. reuteri. After 28 days, the cell viability was above 7 log cfu/g in all creams. L. reuteri ATCC 55730 had the highest viability in cream with 40% pigeon pea and 20% oat (8.16 log cfu/g). In conclusion, due to its acceptability and highly nutritious value, the product could be used so as to support the growth of L. reuteri. PMID:22533458

  1. Respuesta de Gallinas Ponedoras a Raciones con Diferentes Niveles de Harina de Hojas de Guandul (CajanusCajan L.

    Directory of Open Access Journals (Sweden)

    Ruíz V. Juan Guillermo

    1969-09-01

    Full Text Available En el presente trabajo se dan los resultados de dos ensayos llevados a cabo en el Centro Nacional de Investigaciones Agropecuarias de Medellín, para estudiar el efecto producido por la harina de hojas de guandul(Cajanuscajan en la coloración de la yema y en la producción de ponedoras. Durante el primer ensayo se trabajó con 120 gallinas Hy-line en postura avanzada, usando 3 raciones con 74%, 70.5% y68.5%de maíz amarillo como material prima básica y niveles de 0%, 6% y 9% de harina de guandul respectivamente. Aunque a medida que se aumentó el nivel de guandul hubo una pequeña baja en la producción de huevos, esta diferencia no fue significativa, como tampoco lo fue el (consumo de alimento y la coloración de la yema. Esta última fue prácticamente igual en los 3 lotes, debido al alto contenido de maíz amarillo en cada una de las raciones. Para el segundo ensayo se trabajó con 160 gallinas de igual raza, pero con 2 -3 meses de postura y 4 tratamientos: 3 en base a sorgo con 0%, 9% Y 12 % de harina de guandul y un cuarto lote con ración comercial, molida para evitar diferencias. La producción de huevos presentó una diferencia significativamente superior, al nivel 0 .05 %, de los lotes 0%y comercial sobre 9% y 12%. En cuanto al consumo de alimento se encontró diferencia altamente significativa del lote comercial sobre los otros 3 lotes. También el comercial fue el de mayor consumo de alimento por docena de huevos producidos. La coloración de la yema del huevo del lote 0% de guandul fue nula obteniendo un producto de difícil mercado. En los lotes 9%, 12% y comercial fue apropiada y muy semejante la pigmentación. El alimento por docena de huevo durante el segundo ensayo fue mayor en el lote con ración comercial, luego 12 %, 9 % y 0% respectivamente. A los siete días se produjo la pigmentación adecuada en la yema del huevo. Huevos almacenados por 25 días no perdieron su grado de coloración, pero sí su consistencia.

  2. Evaluation and Validation of Housekeeping Genes as Reference for Gene Expression Studies in Pigeonpea (Cajanus cajan) Under Drought Stress Conditions

    OpenAIRE

    Sinha, Pallavi; Vikas K Singh; V Suryanarayana; Krishnamurthy, L.; Saxena, Rachit K.; Varshney, Rajeev K.

    2015-01-01

    Gene expression analysis using quantitative real-time PCR (qRT-PCR) is a very sensitive technique and its sensitivity depends on the stable performance of reference gene(s) used in the study. A number of housekeeping genes have been used in various expression studies in many crops however, their expression were found to be inconsistent under different stress conditions. As a result, species specific housekeeping genes have been recommended for different expression studies in several crop spec...

  3. A Comprehensive Transcriptome Assembly of Pigeonpea (Cajanus cajan L.) using Sanger and Second-Generation Sequencing Platforms

    Institute of Scientific and Technical Information of China (English)

    Himabindu Kudapa; Reetu Tuteja; Trushar Shah; Sutapa Dutta; Deepak K.Gupta; Archana Singh; Kishor Gaikwad; Tilak R.Sharma; Gregory D.May; Nagendra K.Singh; Rajeev K.Varshney; Arvind K.Bharti; Steven B.Cannon; Andrew D.Farmer; Benjamin Mulaosmanovic; Robin Kramer; Abhishek Bohra; Nathan T.Weeks; John A.Crow

    2012-01-01

    A comprehensive transcriptome assembly for pigeonpea has been developed by analyzing 128.9 million short Illumina GA Ⅱx single end reads,2.19 million single end FLX/454 reads,and 18353 Sanger expressed sequenced tags from more than 16 genotypes.The resultant transcriptome assembly,referred to as CcTA v2,comprised 21434 transcript assembly contigs (TACs) with an N50 of 1510 bp,the largest one being ~8 kb.Of the 21434 TACs,16622 (77.5%) could be mapped on to the soybean genome build 1.0.9 under fairly stringent alignment parameters.Based on knowledge of intron junctions,10009 primer pairs were designed from 5033 TACs for amplifying intron spanning regions (ISRs).By using in silico mapping of BAC-end-derived SSR loci of pigeonpea on the soybean genome as a reference,putative mapping positions at the chromosome level were predicted for 6284 ISR markers,covering all 11 pigeonpea chromosomes.A subset of 128 ISR markers were analyzed on a set of eight genotypes.While 116 markers were validated,70 markers showed one to three alleles,with an average of 0.16 polymorphism information content (PIC) value.In summary,the CcTA v2 transcript assembly and ISR markers will serve as a useful resource to accelerate genetic research and breeding applications in pigeonpea.

  4. Effect of pigeon pea (Cajanus cajan L.) on high-fat diet-induced hypercholesterolemia in hamsters.

    Science.gov (United States)

    Dai, Fan-Jhen; Hsu, Wei-Hsuan; Huang, Jan-Jeng; Wu, She-Ching

    2013-03-01

    Obesity is associated with increased systemic and airway oxidative stress, which may result from a combination of adipokine imbalance and antioxidant defenses reduction. Obesity-mediated oxidative stress plays an important role in the pathogenesis of dyslipidemia, vascular disease, and nonalcoholic hepatic steatosis. The antidyslipidemic activity of pigeon pea were evaluated by high-fat diet (HFD) hamsters model, in which the level of high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), total cholesterol (TC), and total triglyceride (TG) were examined. We found that pigeon pea administration promoted cholesterol converting to bile acid in HFD-induced hamsters, thereby exerting hypolipidemic activity. In the statistical results, pigeon pea significantly increased hepatic carnitine palmitoyltransferase-1 (CPT-1), LDL receptor, and cholesterol 7α-hydroxylase (also known as cytochrome P450 7A1, CYP7A1) expression to attenuate dyslipidemia in HFD-fed hamsters; and markedly elevated antioxidant enzymes in the liver of HFD-induced hamsters, further alleviating lipid peroxidation. These effects may attribute to pigeon pea contained large of unsaturated fatty acids (UFA; C18:2) and phytosterol (β-sitosterol, campesterol, and stigmasterol). Moreover, the effects of pigeon pea on dyslipidemia were greater than β-sitosterol administration (4%), suggesting that phytosterone in pigeon pea could prevent metabolic syndrome.

  5. Molecular cloning and characterization of the light-harvesting chlorophyll a/b gene from the pigeon pea (Cajanus cajan).

    Science.gov (United States)

    Qiao, Guang; Wen, Xiao-Peng; Zhang, Ting

    2015-12-01

    Light-harvesting chlorophyll a/b-binding proteins (LHCB) have been implicated in the stress response. In this study, a gene encoding LHCB in the pigeon pea was cloned and characterized. Based on the sequence of a previously obtained 327 bp Est, a full-length 793 bp cDNA was cloned using the rapid amplification of cDNA ends (RACE) method. It was designated CcLHCB1 and encoded a 262 amino acid protein. The calculated molecular weight of the CcLHCB1 protein was 27.89 kDa, and the theoretical isoelectric point was 5.29. Homology search and sequence multi-alignment demonstrated that the CcLHCB1 protein sequence shared a high identity with LHCB from other plants. Bioinformatics analysis revealed that CcLHCB1 was a hydrophobic protein with three transmembrane domains. By fluorescent quantitative real-time polymerase chain reaction (PCR), CcLHCB1 mRNA transcripts were detectable in different tissues (leaf, stem, and root), with the highest level found in the leaf. The expression of CcLHCB1 mRNA in the leaves was up-regulated by drought stimulation and AM inoculation. Our results provide the basis for a better understanding of the molecular organization of LCHB and might be useful for understanding the interaction between plants and microbes in the future.

  6. Genetic analysis of yield and its component traits in drought tolerant genotypes of pigeonpea (Cajanus cajan L. Millspaugh

    Directory of Open Access Journals (Sweden)

    Ch. Sreelakshmi, C.V. Sameer Kumar and D. Shivani

    2010-12-01

    Full Text Available In the present investigation, sufficient variability was observed in the drought tolerant pigeonpea genotypes for the traits under study. Most of theyield contributing characters showed significant positive correlation whereas, number of primary branches per plant showed significant negativecorrelation with seed yield. Days to maturity had maximum direct effect on seed yield followed by number of pods per plant and plant height andnumber of secondary branches which had negative direct effect on seed yield. Number of secondary branches per plant, number of pods per plantand days to maturity were identified as important yield components and hence, selection should be focused on these traits to improve yield indrought tolerant genotypes of pigeonpea.

  7. Genetic diversity studies and identification of SSR markers associated with Fusarium wilt (Fusarium udum) resistance in cultivated pigeonpea (Cajanus cajan)

    Indian Academy of Sciences (India)

    A. K. Singh; V. P. Rai; R. Chand; R. P. Singh; M. N. Singh

    2013-08-01

    Genetic diversity and identification of simple sequence repeat markers correlated with Fusarium wilt resistance was performed in a set of 36 elite cultivated pigeonpea genotypes differing in levels of resistance to Fusarium wilt. Twenty-four polymorphic sequence repeat markers were screened across these genotypes, and amplified a total of 59 alleles with an average high polymorphic information content value of 0.52. Cluster analysis, done by UPGMA and PCA, grouped the 36 pigeonpea genotypes into two main clusters according to their Fusarium wilt reaction. Based on the Kruskal–Wallis ANOVA and simple regression analysis, six simple sequence repeat markers were found to be significantly associated with Fusarium wilt resistance. The phenotypic variation explained by these markers ranged from 23.7 to 56.4%. The present study helps in finding out feasibility of prescreened SSR markers to be used in genetic diversity analysis and their potential association with disease resistance.

  8. Dicty_cDB: Contig-U03348-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available QU... 38 0.12 2 ( FI228353 ) CccaBa045-E11TV CccABa Cajanus cajan genomic clon... 48 0.21 1 ( FI218856 ) CccaBa046-J13TR CccABa Cajan...us cajan genomic clon... 48 0.21 1 ( FI198911 ) CccaBa012-M12TR CccABa Cajanus... cajan genomic clon... 48 0.21 1 ( FI198280 ) CccaBa008-J22TV CccABa Cajanus cajan gen

  9. Nutrients and certain lipid soluble bioactive components in dehusked whole grains (gota) and dehusked splits (dhal) from pigeon pea (Cajanus cajan) and their cooking characteristics.

    Science.gov (United States)

    Jayadeep, Padmanabhan A; Sashikala, Vadakkoot B; Pratape, Vishwas M

    2009-01-01

    The nutritional quality of dehusked whole grains (gota) and dehusked splits (dhal) in red and white varieties of pigeon pea regarding proximate composition and certain lipid-soluble bioactive components was investigated. A decrease in fat and crude fiber was noticed when gota was converted to dhal. The lipid profile of gota and dhal from red and white husk pigeon pea types indicated that essential fatty acids were greater in gota than in their respective dhals. Gota from white husk variety contained more tocopherols than the red variety. Dhal contained less tocopherols than gota. A decrease in the content of gamma and alpha tocopherols, vitamin E activity and total antioxidant activity also indicates loss of bioactive components on splitting gota into dhal. Cooking time and dispersed solids on cooking indicated good cooking quality of gotta. The results indicated the nutritional superiority of gota over dhal and its similarity with dhal in cooking characteristics.

  10. Chloroplast pigments, proteins, lipid peroxidation and activities of antioxidative enzymes during maturation and senescence of leaves and reproductive organs of Cajanus cajan L.

    Science.gov (United States)

    Jakhar, Somveer; Mukherjee, D

    2014-04-01

    A comparative investigation was undertaken with pigeon pea leaves and attached flower buds/flowers/pods during their developmental stages including senescence in a natural system in experimental plots. Alterations in chloroplast pigments, total soluble proteins, lipid peroxidation, malondialdehyde (MDA) content and activities of guaiacol peroxidase (POD, EC 1.11.1.7) and superoxide dismutase (SOD, EC 1.15.1.1) were studied at 5-day interval from initial to 40-day stage. Chloroplast pigments and proteins of leaves increased upto 15 and 20-day stages respectively followed by a steady decline. Reproductive parts, however, exhibited rise in chloroplast pigments upto 25-day and protein till last stage as developing pods gain the amount from the senescing leaves which are nearest to them. Senescing leaves show very high POD activity than the developing and senescing pods and POD appears to be associated with chlorophyll degradation. Considerably higher activity and amount of LOX and MDA respectively have been noticed in senescing leaves than in flowers and pods. Increase in SOD activity during early stage of leaf growth and maturation indicates protective role that declined at senescent stages. Pods are unique in having very high SOD activity, only last stage of senescence does show a decline. PMID:24757321

  11. 木豆育种及分子生物学研究进展%Advances in Breeding and Molecular Biology Research of Pegionpea [Cajanus cajan (L.) Millsp.

    Institute of Scientific and Technical Information of China (English)

    唐军; 王文强; 黄春琼; 白昌军

    2013-01-01

    木豆为豆科木豆属多年生常绿小灌木,是世界第六大食用豆类,也是唯一的木本食用豆类.木豆用途非常广泛,成熟籽实可以食用,营养价值丰富;嫩枝叶是草食家畜的优质饲料,又可用作水土保持和覆盖作物等.木豆对土壤条件要求不严,适合世界热带亚热带地区种植.本文介绍国内外木豆育种及分子生物学研究概况和进展,并对国内今后木豆研究提出展望.

  12. NAC transcription factor genes: genome-wide identification, phylogenetic, motif and cis-regulatory element analysis in pigeonpea (Cajanus cajan (L.) Millsp.).

    Science.gov (United States)

    Satheesh, Viswanathan; Jagannadham, P Tej Kumar; Chidambaranathan, Parameswaran; Jain, P K; Srinivasan, R

    2014-12-01

    The NAC (NAM, ATAF and CUC) proteins are plant-specific transcription factors implicated in development and stress responses. In the present study 88 pigeonpea NAC genes were identified from the recently published draft genome of pigeonpea by using homology based and de novo prediction programmes. These sequences were further subjected to phylogenetic, motif and promoter analyses. In motif analysis, highly conserved motifs were identified in the NAC domain and also in the C-terminal region of the NAC proteins. A phylogenetic reconstruction using pigeonpea, Arabidopsis and soybean NAC genes revealed 33 putative stress-responsive pigeonpea NAC genes. Several stress-responsive cis-elements were identified through in silico analysis of the promoters of these putative stress-responsive genes. This analysis is the first report of NAC gene family in pigeonpea and will be useful for the identification and selection of candidate genes associated with stress tolerance. PMID:25108674

  13. Selection and Validation of Housekeeping Genes as Reference for Gene Expression Studies in Pigeonpea (Cajanus cajan) under Heat and Salt Stress Conditions.

    Science.gov (United States)

    Sinha, Pallavi; Saxena, Rachit K; Singh, Vikas K; Krishnamurthy, L; Varshney, Rajeev K

    2015-01-01

    To identify stable housekeeping genes as a reference for expression analysis under heat and salt stress conditions in pigeonpea, the relative expression variation for 10 commonly used housekeeping genes (EF1α, UBQ10, GAPDH, 18Sr RNA, 25Sr RNA, TUB6, ACT1, IF4α, UBC, and HSP90) was studied in root, stem, and leaves tissues of Asha (ICPL 87119), a leading pigeonpea variety. Three statistical algorithms geNorm, NormFinder, and BestKeeper were used to define the stability of candidate genes. Under heat stress, UBC, HSP90, and GAPDH were found to be the most stable reference genes. In the case of salinity stress, GAPDH followed by UBC and HSP90 were identified to be the most stable reference genes. Subsequently, the above identified genes were validated using qRT-PCR based gene expression analysis of two universal stress-resposive genes namely uspA and uspB. The relative quantification of these two genes varied according to the internal controls (most stable, least stable, and combination of most stable and least stable housekeeping genes) and thus confirmed the choice as well as validation of internal controls in such experiments. The identified and validated housekeeping genes will facilitate gene expression studies under heat and salt stress conditions in pigeonpea. PMID:27242803

  14. The technology of homogenate extraction of Cajanus polysaccharide in Cajanus Cajan (L.) Millsp%木豆叶中木豆多糖提取工艺优选

    Institute of Scientific and Technical Information of China (English)

    王惠娟; 史伟国; 田丽华

    2012-01-01

    目的:以木豆为原料,对匀浆法提取木豆中多糖工艺进行研究,确定其最佳工艺务件,为木豆药用资源研究与开发提供可靠依据.方法:采用正交设计的方法,以匀浆时间、匀浆次数、料液比为考察因素,优选最佳匀浆提取工艺条件.结果:木豆多糖提取最佳工艺参数为 匀浆时间9min、匀浆次数3次、料液比1∶30(g∶mL),在最佳条件下,木豆多糖得率为12.27%.结论:匀浆提取法操作简单、减少粉尘污染、提取率高、提取时间短,是一种高效提取木豆多糖的方法.

  15. Cloning and Sequence Analysis of Actin Gene Fragment from Cajanus cajan%木豆Actin基因片段的克隆及序列分析

    Institute of Scientific and Technical Information of China (English)

    乔光; 文晓鹏

    2010-01-01

    根据大豆Actin基因的保守序列设计一对引物Primer 1和Primer 2,以木豆叶片总RNA为模板,采用RT-PCR的方法扩增出Actin基因片段并克隆到PMD-18T载体.阳性克隆经PCR检测后测序,序列分析结果表明,该片段长302 bp,编码100个氨基酸;所得序列与GenBank中注册的其他植物Actin基因核苷酸序列的同源性均在89%以上,其中与大豆的同源性达94%;与氨基酸序列的同源性均在90%以上.

  16. Selection and Validation of Housekeeping Genes as Reference for Gene Expression Studies in Pigeonpea (Cajanus cajan) under Heat and Salt Stress Conditions

    OpenAIRE

    Sinha, Pallavi; Saxena, Rachit K.; Singh, Vikas K.; Krishnamurthy, L.; Rajeev K Varshney

    2015-01-01

    To identify stable housekeeping genes as a reference for expression analysis under heat and salt stress conditions in pigeonpea, the relative expression variation for 10 commonly used housekeeping genes (EF1α, UBQ10, GAPDH, 18Sr RNA, 25Sr RNA, TUB6, ACT1, IF4α, UBC, and HSP90) was studied in root, stem, and leaves tissues of Asha (ICPL 87119), a leading pigeonpea variety. Three statistical algorithms geNorm, NormFinder, and BestKeeper were used to define the stability of candidate genes. Unde...

  17. 木豆新品种产量对比及适应性分析%Grain Yield and Adaptability of Cajanus cajan New Varieties

    Institute of Scientific and Technical Information of China (English)

    李正红; 周朝鸿; 谷勇; 刘富泰; 和金福; 李福强

    2005-01-01

    以地方栽培品种为对照,在云南省元江县、泸水县、云县对从印度国际干旱热带作物研究所引进的9个木豆新品种进行了产量、稳定性及适生性鉴定.结果表明:参试品种中V7、V5、V6产量明显高于对照,且稳定性好,变异小,最值得推广;V10、V3、V2 3个品种产量比对照稍高,但变异大,应择其适宜地区推广;V8、V1、V9 3个品种虽产量低于对照,但未达显著水平,其中V1、V9品质最好,荚大,粒大,鲜粒甜度优于豌豆,可作新鲜蔬菜,仍可在环境类似于地点2(泸水)的地区加以推广.通过地点效应分析确定,类似于泸水县的环境较适宜木豆栽培.

  18. Effect of incorporation of decorticated pigeon pea (Cajanus cajan protein isolate on functional, baking and sensory characteristics of Wheat (Triticum aesitivum biscuit

    Directory of Open Access Journals (Sweden)

    H. A. Hassan

    2013-08-01

    Full Text Available This study was undertaken with the objectives of using the decorticated pigeon pea protein isolate in the development of protein rich-biscuit, suitable for general and specific nutritional purposes and to study the effect of incorporation of pigeon pea protein isolate on the sensory evaluation and quality of biscuit produced. Decorticated Pigeon Pea protein Isolate (DPPI was incorporated in wheat (Triticum aesitivum flour (WF, extraction rate 72%, for making fortified biscuit. Ratios of DPPI in wheat flour were adjusted to protein levels of 15, 20 and 25%, respectively. Rheological and functional properties as well as proximate composition, nutritive value and sensory characteristics for the biscuit produced were assessed. The gluten quantity (dry and wet and falling number of wheat flour were significantly (p#0.05 decreased with the incorporation of DPPI from 10.25 to 7.6%; from 31.2 to 22.5% and from 657 to 443 sec., for 0,15, 20 and 25% protein levels, respectively.Water Retention Capacity (WRC, Bulk Density (BD and Fat Absorption Capacity (FAC were obtained. Addition of DPPI resulted in an increase in water absorption which was found to be ranged from 66.7 to 71.0%; dough development time from 4.5 to 7.3 min and dough stability 1.7 to 5.8 min. Biscuit supplemented with DPPI showed significant increase (p≤0.05 in ash, protein with high level of incorporation and significant decrease (p≤0.05 in carbohydrates and caloric values when biscuit wheat was supplemented with high level of DPPI. Incorporation of DPPI showed no significant differences (p≤0.05 on biscuit spread ratios compared to wheat biscuit (control. Biscuit with 15% protein level was found to be superior in all its sensory characteristics compared to the other blends.

  19. 元谋干热区两年生木豆(Cajanus cajan)人工林营养循环和养分利用效率%The nutrient cycling and the efficiency of nutrient utilization of the Cajanus cajan 2-year old plantation in Dry-hot Region

    Institute of Scientific and Technical Information of China (English)

    拜得珍; 纪中华; 沙毓沧

    2007-01-01

    通过样方调查和采样,对元谋干热区2年生不同种植密度木豆人工林养分的营养循环和养分利用效率进行了研究.结果表明,不同种植密度下林分的营养循环表现出了一定的差异性,而养分的利用效率则一样.0.8×0.8m2、1.0×0.8m2和1.0×1.0m2种植密度下林分的养分吸收量分别为1497.64、1326.26和1046.00kg/(hm2 · a),其中从土壤中年吸收养分量以Ca最多,N、K次之,Mg、P再次,Fe、Cu最少,且随种植密度的增大呈增加趋势;养分的归还量分别为476.30、729.95和518.98 kg/(hm2 · a); 存留量分别为753.34、596.3和527.07 kg/(hm2 · a);大量元素养分的循环速率分别为0.49、0.55和0.49,微量元素养分循环速率分别为0.79、0.83和0.80.据此提出,对于生长旺盛后期的林分,采取适量施肥和调整林分密度等经营措施,提高养分的利用效率,增加林分对养分的吸收量和存留量.同时得出1.0×0.8m2的种植密度更有利于系统内营养元素的生物循环、养分利用效率和保持地力持久.与油松、刺槐、板栗、锥栗等相比,木豆对养分的利用效率明显偏低.

  20. Coast cross (Cynodon dactylon (L. pers. hay and pigeon pea (Cajanus cajan (L. millsp digestibility and nutrients average intake by sheep under two feeding systems/ Consumo médio e digestibilidade do feno de capim “Coast cross” (Cynodon dactylon (L. pers. e feijão guandu (Cajanus cajan (L. Millsp em carneiros submetidos a dois regimes alimentares

    Directory of Open Access Journals (Sweden)

    Luiz Waldemar de Oliveira Souza

    2007-08-01

    Full Text Available This experiment was carried out with the objectives of determining the daily average intake (DAI, apparent digestibility coefficient (ADC of coast cross hay and pigeon pea nutrients and to evaluate thenitrogen balance in sheep. Ten wethers with average weight of 27.50 kg, alloted to suitable cages, were used. A completely randomized design with two treatments (T1= 80% coast cross hay + 20% pigeon peaand T2= 60% coast cross hay + 40% pigeon pea and five replicates, was used. The treatments were evaluated under two feeding systems (voluntary and restrict. Animals receiving ration of T2 presentedhigher DAI (g/kg PV 0.75 (PO experimento foi realizado com os objetivos de determinar o consumo médio diário (CMD, o coeficiente de digestibilidade aparente (CDA dos nutrientes do feno de capim Coast cross e feijão guandu e avaliar o balanço de nitrogênio (BN em ovinos. Foram utilizados dez ovinos machos, castrados, com peso médio de 27,50 kg, alojados em gaiolas para metabolismo, em delineamento inteiramente casualizado com dois tratamentos (T1 = 80% de feno de Coast cross + 20% de guandu e T2 = 60% de feno de Coast cross + 40% de guandu e cinco repetições por tratamento. Os tratamentos foram avaliados sob dois regimes alimentares (consumo voluntário e restrito. Os animais recebendo ração do T2 apresentaram maior CMD (g/kg PV0,75 (p < 0,05 de matéria seca (MS, proteína bruta (PB, fibra bruta (FB, matéria orgânica (MO e extrato não nitrogenado (ENN, do que aqueles animais recebendo ração do T1. O CDA da FB da ração T2 foi maior (p < 0,05 do que o da ração T1. O BN dos animais que receberam 40% de guandu (13,15g N/ dia foi superior aos daqueles que receberam 20% desta leguminosa (10,29g N/dia. Conclui-se que o feijão guandu pode ser utilizado como fonte protéica na alimentação de ruminantes, possibilitando CDA da MS próximo de 52% para animais em regime de consumo voluntário, e 81% para animais em regime de consumo restrito. O aumento na porcentagem de feijão guandu nas dietas de ovinos alimentados com feno de Coast-cross pode resultar em melhoria no CDA da FB da dieta, bem como no balanço de nitrogênio dos animais.

  1. Influência do tempo de armazenamento a 11°C sobre algumas características físicas de cultivares de feijão guandu (Cajanus cajan L. Influence of storage time at 11ºC on some physical characteristics of guandu beans (Cajanus cajan L. cultivars

    Directory of Open Access Journals (Sweden)

    S.G. CANNIATTI-BRAZACA

    1998-04-01

    Full Text Available Foram avaliadas as seguintes características físicas: densidade, água absorvida e tempo de cocção de 33 cultivares de feijão guandu. A densidade ficou estável durante o armazenamento entre os cultivares. A relação entre a massa hidratada pela massa inicial foi acima de 2,0 até aos 6 meses para a maioria dos cultivares sendo que aos 9 meses tivemos algumas cultivares que não atingiram relação acima de 2,0 e aos 12 meses somente 2 cultivares atingiram relação 2,0. A relação do tempo de cocção inicial pelo tempo de cocção em diferentes tempos demonstrou comportamentos distintos entre os cultivares, sendo que os cultivares L-16 e L-14 apresentaram as maiores relações entre o tempo inicial e o período de 12 meses de armazenagem. .Por esses resultados, pôde-se observar a grande variabilidade das características físicas dos diferentes cultivares estudados. No entanto houve a possibilidade de selecionar as cultivares L-03; L-09; L-29; L-30 e L-36 por apresentarem maior estabilidade frente ao tempo de armazenamento.Physical characteristics as density, water absorbed, and cooking time of 33 cultivars of guandu beans were evaluated. Density was stable during storage among cultivars. The ratio between the initial hydrated mass was over 2.0 until 6 months for most cultivars and the ratio of some cultivars failed to reach over 2.0 at 9 months and at 12 months only 2 cultivars reached a 2.0 ratio. The ratio of initial cooking time to the cooking time at different times showed distinct behavior among cultivars and cultivars L-16 and L-14 had the highest ratios between initial time and a 12-month storage period. These results showed the great variability of the physical characteristics of the varied cultivars studied. Cultivars L-03, L-09, L-29, L-30, and L-36, however, were likely to be selected due to their higher stability concerning storage time.

  2. Aspectos tecnológicos e sensoriais do guandu [Cajanus cajan (L. Millsp.] enlatado em diferentes estádios de maturação Technological and sensorial aspects of pigeon pea [Cajanus cajan (L. Millsp.] canned at different stages of maturation

    Directory of Open Access Journals (Sweden)

    M.F.P. BARCELOS

    1999-01-01

    Full Text Available O objetivo deste trabalho é viabilizar a introdução do guandu verde enlatado no mercado brasileiro. Foram realizadas três colheitas de grãos ainda verdes da cultivar IAC Fava Larga obtidos no 44º, 57º e 62º dia após a floração (DAF e finalmente no 92º DAF, quando os grãos já possuíam cor pardo-amarelada. Para todos os lotes, após otimização das condições de enlatamento, 121ºC durante 5 a 6 minutos, efetuaram-se estudos quanto ao acúmulo de matéria seca, composição dos grãos, cor, textura e aceitação sensorial. Conclui-se, através do acúmulo de matéria seca do grão, que a maturidade fisiológica ocorreu em torno do 62º DAF, quando também apresentou as melhores características para o enlatamento. As cores dos grãos pós-enlatamento, vermelha e amarela (unidades Lab Hunter apresentaram estáveis até o 62º DAF. A textura foi gradativamente mais firme com o avanço do amadurecimento tendo verificado correlação positiva (r = 0,96 entre medidas de texturas sensorial e instrumental. A aceitação do guandu enlatado cultivar IAC Fava Larga foi apenas regular.The objective of this work is to enable the introduction of canned green pigeon pea in Brazilian market. Three harvests of still green grains of the cultivar IAC Fava Larga, obtained on the 44th, 57th and 62nd days after flowering, DAF, and finally on the 92nd DAF when the grains already possessed yellowish dusky color, were undertaken. To all the lots, after optimization of canning conditions, 121ºC for 5 to 6 minutes, studies concerning the accumulation of dry matter, grain composition, color, texture and sensorial acceptance were carried out. It was concluded through the accumulation of dry matter of the grain, that the physiological maturity occurred around the 62nd DAF. The texture was gradually firmer with advancing maturation, and positive correlation having been found (r = 0,96 between sensorial and instrumental texture measures was noticed. The acceptance of the canned pigeon pea cultivar IAC Fava Larga was only regular.

  3. Contribuição da fixação simbiótica de nitrogênio na produção do guandu (Cajanus cajan (L.) Millsp) Contribution of symbiotic nitrogen fixation on pigeon-pea yield (Cajanus cajan (L.) Millsp)

    OpenAIRE

    M.J. Valarini; R GODOY

    1994-01-01

    Experimento com guandu foi conduzido em casa-de-vegetação utilizando latossolo vermelho-amarelo variação Laras no Instituto de Zootecnia, Nova Odessa, SP, para avaliar parâmetros indiretos de fixação simbiótica de N2. As plantas utilizadas representam acessos de guandu obtidos no CPPSE-EMBRAPA, São Carlos, SP, selecionados para características agronômico-forrageiras. Matéria seca da parte aérea, matéria seca de nódulos, atividade de redução de acetileno, eficiência relativa e teor de nitrogên...

  4. Contribuição da fixação simbiótica de nitrogênio na produção do guandu (Cajanus cajan (L. Millsp Contribution of symbiotic nitrogen fixation on pigeon-pea yield (Cajanus cajan (L. Millsp

    Directory of Open Access Journals (Sweden)

    M.J. Valarini

    1994-12-01

    Full Text Available Experimento com guandu foi conduzido em casa-de-vegetação utilizando latossolo vermelho-amarelo variação Laras no Instituto de Zootecnia, Nova Odessa, SP, para avaliar parâmetros indiretos de fixação simbiótica de N2. As plantas utilizadas representam acessos de guandu obtidos no CPPSE-EMBRAPA, São Carlos, SP, selecionados para características agronômico-forrageiras. Matéria seca da parte aérea, matéria seca de nódulos, atividade de redução de acetileno, eficiência relativa e teor de nitrogênio total da parte aérea não diferiram significativamente (P Pigeon-pea accessions were grown in a yellow-red latossol var. Laras in a greenhouse at the Instituto de Zootecnia, Nova Odessa, SP, Brazil, to evaluate their N2 fixing characteristics. Shoot dry matter weight, mass of nodules, acetylene reduction activity, dry matter N content and relative efficiency were not significantly increased by the inoculation with recommended strains of Bradyrhizobium spp. However, inoculation made shoot weight and nitrogen content (15 - 17% crude protein comparable to the nitrogen fertilization level of 120 kg N/ha. It is concluded that the pigeon-pea can establish a Bradyrhizobium association able to provide more than 90% of nitrogen needed for its vegetative growth.

  5. Dicty_cDB: Contig-U05042-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available a japonicum SJCHGC06716 protein mRNA, p... 48 0.20 1 ( FI266769 ) CccaBb064-M7JR CccABb Cajanus cajan genomi...c clone... 48 0.20 1 ( FI249869 ) CccaBb013-J14JR CccABb Cajanus cajan genomic clon... 48 0.20 1 ( FI234268 ...) CccaBb023-E13JF CccABb Cajanus cajan genomic clon... 48 0.20 1 ( FI203533 ) CccaBa004-K10TV CccABa Cajan... 46 0.80 1 ( FI200335 ) CccaBa030-K13TR CccABa Cajanus cajan genomic clon... 46 0.80 1 ( FI114105 ) MUGQ_CH2...us cajan genomic clon... 48 0.20 1 ( AL976180 ) Danio rerio genomic clone DKEY-102J

  6. Dicty_cDB: Contig-U05240-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available nomic DNA, chromoso... 44 0.42 2 ( FI202420 ) CccaBa022-F12TV CccABa Cajanus cajan genomic clon... 46 1.4 1 ...QUE... 44 5.5 1 ( FI227150 ) CccaBa043-H4TV CccABa Cajanus cajan genomic clone... 44 5.5 1 ( FI225933 ) CccaBa055-O10TV CccABa Cajanu...s cajan genomic clon... 44 5.5 1 ( FI209274 ) CccaBa053-G1TR CccABa Cajanus cajan g

  7. Dicty_cDB: Contig-U01636-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available us cajan genomic clon... 46 2.9 1 ( FI207266 ) CccaBa021-J1TR CccABa Cajanus...SEQU... 46 2.9 1 ( AC169006 ) Sorex araneus clone SA_Ba-102E5, WORKING DRAFT SE... 46 2.9 1 ( FI239516 ) CccaBb022-H13JR CccABb Cajan

  8. Dicty_cDB: Contig-U01737-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available embryo clone:m... 32 4.1 3 ( FI259718 ) CccaBb042-A12JF CccABb Cajanus cajan genomic clon... 36 5.0 2 ( AC1...... 38 9.4 2 ( FI248628 ) CccaBb026-D13JR CccABb Cajanus cajan genomic clon... 36

  9. Dicty_cDB: Contig-U01201-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .0 1 ( FI457626 ) CH322-28N20.x CHORI-322 Drosophila melanogaster g... 44 9.0 1 ( FI204272 ) CccaBa013-B4TR CccABa Cajanus... cajan genomic clone... 44 9.0 1 ( FI199123 ) CccaBa031-F5TR CccABa Cajanus cajan genomic clon

  10. Dicty_cDB: Contig-U02301-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available amoeba histolytica Sheared DNA Entam... 44 7.6 1 ( FI224294 ) CccaBa050-J3TV CccABa Cajanus... cajan genomic clone... 44 7.6 1 ( FI201490 ) CccaBa022-I9TV CccABa Cajanus cajan genomic clone...

  11. Dicty_cDB: VFL417 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 17 ( FI217199 ) CccaBa060-L24TR CccABa Cajanus cajan genomic clon... 50 0.047 2 (... FI199692 ) CccaBa031-N10TR CccABa Cajanus cajan genomic clon... 50 0.047 2 ( EJ424712 ) 1093012257626 Globa

  12. Dicty_cDB: Contig-U01537-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available us laevis cDNA clone IMAGE:5514563, partial ... 46 2.6 1 ( FI249612 ) CccaBb031-I23JF CccABb Cajanus cajan g...enomic clon... 46 2.6 1 ( FI194717 ) CccaBa016-G17TR CccABa Cajanus cajan genomic clon... 46 2.6 1 ( EK14004

  13. Dicty_cDB: Contig-U04579-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 75 1 ( FI266530 ) CccaBb034-P16JR CccABb Cajanus cajan genomic clon... 46 0.75 1 ...( FI252525 ) CccaBb017-J13JR CccABb Cajanus cajan genomic clon... 46 0.75 1 ( FI072106 ) CHO_OF7325xd20f1.ab

  14. Dicty_cDB: Contig-U05106-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Homo sapiens cDN... 34 0.83 2 ( FI237908 ) CccaBb014-E5JR CccABb Cajanus cajan genomic clone... 30 1.1 3 ( F...I266759 ) CccaBb064-K7JR CccABb Cajanus cajan genomic clone... 30 1.1 3 ( CS416754 ) Sequence 7 from Patent

  15. Dicty_cDB: Contig-U01103-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available s clone R3-4002I6, WO... 34 1.3 8 ( FI267977 ) CccaBb038-H3JF CccABb Cajanus cajan genomic clone... 34 1.5 2... ( FI260421 ) CccaBb035-J15JF CccABb Cajanus cajan genomic clon... 34 1.5 2 ( AL445233 ) Human DNA sequence

  16. Dicty_cDB: AFM729 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available stelium discoideum cDNA clone:ddc27c19, 3' ... 40 0.042 2 ( FI244407 ) CccaBb004-H23JF CccABb Cajanus... cajan genomic clon... 50 0.20 1 ( FI237621 ) CccaBb025-A5JR CccABb Cajanus cajan genomi

  17. Dicty_cDB: Contig-U15652-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available C163421 ) Bos taurus clone CH240-141B7, *** SEQUENCING IN P... 46 2.6 1 ( FI223731 ) CccaBa058-P11TV CccABa Cajanus... cajan genomic clon... 46 2.6 1 ( FI221562 ) CccaBa035-P10TV CccABa Cajanus cajan genomic clon... 46

  18. Dicty_cDB: Contig-U04877-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 267210 ) CccaBb044-F6JR CccABb Cajanus cajan genomic clone... 44 3.4 1 ( FI261547... ) CccaBb058-D7JF CccABb Cajanus cajan genomic clone... 44 3.4 1 ( FH001788 ) CHO_OF276xk12f2.ab1 CHO_OF Nic

  19. Dicty_cDB: Contig-U05096-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Microcebus murinus clone CH257-397E23, WORKING DR... 44 6.0 1 ( FI267105 ) CccaBb044-B7JR CccABb Cajanus ca...jan genomic clone... 44 6.0 1 ( FI266561 ) CccaBb037-G2JF CccABb Cajanus cajan genomic clone... 44 6.0 1 ( C

  20. Dicty_cDB: Contig-U03648-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available r... 46 1.3 1 ( FI502336 ) AHT1B5RGH004-J15TP AHT1BS-Rearray Arachis hypogae... 46 1.3 1 ( FI250412 ) CccaBb008-B4JF CccABb Cajanus... cajan genomic clone... 46 1.3 1 ( FI229553 ) CccaBa045-F2TV CccABa Cajanus cajan gen...omic clone... 46 1.3 1 ( FI217665 ) CccaBa051-E6TR CccABa Cajanus cajan genomic clone... 46 1.3 1 ( FI200028... ) CccaBa023-L7TV CccABa Cajanus cajan genomic clone... 46 1.3 1 ( FH534344 ) CHO..... 44 4.9 1 ( FI231205 ) CccaBa046-I13TV CccABa Cajanus cajan genomic clon... 44 4.9 1 ( FI215607 ) CccaBa047-I6TR CccABa Cajanus

  1. A Preliminary Study on Preparation of Super Sluper of Cross- Linking Starch from Cajanus cajan Graft Acrylic Acid%木豆淀粉接枝丙烯酸制备超强吸水剂研究初探

    Institute of Scientific and Technical Information of China (English)

    张建云; 李正红; 周朝鸿; 刘秀贤; 惠雅玲; 邱坚; 和润喜; 齐先锋

    2001-01-01

    以木豆淀粉为原料,在引发剂的作用下,利用接枝丙烯酸制备超强吸水剂;对主要影响因子进行了正交设计,并优化工艺参数,最终得到吸水倍数为200~250倍的超强吸水剂.

  2. Occurrence Characteristics and Damage Level of Pests and Diseases of Cajanus cajan in Guizhou%贵州木豆病虫害发生特点及危害程度

    Institute of Scientific and Technical Information of China (English)

    周明强; 龚德勇; 班秀文; 刘凡值; 周正邦

    2009-01-01

    为摸清木豆病虫害在贵州种植区的发生危害情况,采取多点多次抽样法对贵州省木豆病虫害发生情况进行了调查,并收集标本进行室内分类鉴定.结果:共鉴定出病害5种,其中真菌性病害4种,病毒性病害1种;害虫39种,其中昆虫纲7目19科37种,蛛形纲1目2科2种.着重介绍了在贵州种植区危害较重的3种病害、6种虫害的生物学特征及危害特点.可为贵州省木豆大面积推广种植及病虫害防治提供参考依据.

  3. 新疆干旱区木豆引种适应性研究%Adaptability of Pigeon Pea(Cajanus cajan)Introduced in the Arid Areas of Xinjiang

    Institute of Scientific and Technical Information of China (English)

    窦中江; 刘辉; 李铭

    2008-01-01

    对我国西南地区木豆品种进行了引种栽培试验,首次研究木豆在新疆干旱区栽培的适应性.获得了各木豆品种在新疆干旱区的物候期及生长规律,测定了各品种生物产量和鲜枝叶营养成分.栽培试验获得较高的鲜枝叶产量,木豆鲜叶的蛋白质和粗纤维含量较高.但各木豆品种均不能完成生育期,无法获得成熟果实,不能越冬.

  4. Effects of Cajanus Cajan(L.) Millsp on vascular endothelial function of ovariectomized rats%木豆叶对去卵巢大鼠血管内皮细胞功能的影响

    Institute of Scientific and Technical Information of China (English)

    刘彦凤; 袁捷; 全世建; 侯少贞; 周才杰; 郭芳芳; 赖小平

    2009-01-01

    目的 观察木豆叶对卵巢切除大鼠血管内皮细胞功能的影响.方法 采用双侧卵巢摘除法诱导骨质疏松症模型,术后3 d开始防治性给药,于第9 w眼球采血,放射免疫法检测内皮素,硝酸还原酶法检测一氧化氮.结果 血浆ET水平升高,血清NO含量降低,与假手术组比较有统计学意义(P<0.01); 龙牡壮骨颗粒组、木豆叶高、中、低剂组都不同程度降低ET水平,增加NO含量,与模型组比较有统计学意义(P<0.01或P<0.05).结论 木豆叶通过调节ET、NO的含量提高去势大鼠血管内皮功能.

  5. 木豆贮藏蛋白基因的克隆及其序列分析%Cloning and Analysis of the Gene Encoding Glycilin from Pigeonpea[Cajanus cajan(L.) Millsp.

    Institute of Scientific and Technical Information of China (English)

    唐军; 王文强; 白昌军; 金志强

    2012-01-01

    A special cDNA fragments with 833 base pairs(bp) long from the pigeonpea material was clone by means of designing degenerate primers homology to the sequences of other leguminous plants, RT -PCR amplification and agarose gel electrophoresis, blast tools. The full-length 1 595 bp cDNA encoding pigeonpea glycinlin(PPGLY)was cloned by RT-PCR, 3' and 5RACE technology. It contained a 1 551 bp(16 to 1 566 bp)ORF, encoding 516 ammo acids with PPGLY molecular weight 57.718 ku of an isoelectric point(pl)4.68. The deduced amino acid sequence of PPGLY showed high homologies 83%, 79%, 77%, 73%, 67%, 65% and 65% with Glycine mm (1303273A), Glycine max (CAA55977.1), Lupinus angxatifolius (AEB33710.1), Arachis hypogaea (AAU21493.1), Phaseolus vulgaris (ADR30064.1), Vicia fata/(CAA83674.1), Medicago tiuncatula(XP_003590690.1), respectively.%根据几种豆科植物贮藏蛋白基因序列同源性设计简并引物,通过反转录扩增,经琼脂糖凝胶电泳检测和测序,并利用NCBI数据库及Blast工具进行分析,从木豆中克隆得到1个贮藏蛋白基因的cDNA片断,长度为833 bp.采用RACE、RT-PCR技术扩增得到该基因的全长cDNA,含1 595 bp,推测编码区为1551 bp(16-1566bp),编码516个氨基酸,分子量为57.718 ku,等电点为4.68.该基因推导的氨基酸与大豆(1303273A)、野生大豆(CAA55977.1)、羽扇豆(AEB33710.1)、花生(AAU21493.1)、菜豆(ADR30064.1)、野豌豆(CAA83674.1)为、截形苜蓿(XP_003590690.1)的贮藏蛋白同源性分别达到83%、79%、77%、73%、67%、65%、65%.

  6. Influence of Land Preparation on Growth and Seed Setting of Cajanus cajan%不同整地方法对木豆植株生长及结实的影响

    Institute of Scientific and Technical Information of China (English)

    谷勇; 李福强; 刘富泰; 和金福; 石晶荣; 李正红

    2003-01-01

    对印度引进的木豆晚熟品种ICP7035,在怒江六库进行了不同整地方法的造林试验.试验结果表明:木豆的单株产量以全垦整地方法为最高达0.064 kg/株,打塘整地方法最低为0.038 kg/株.通过投入与产出的经济效益分析,得出木豆单位面积的经济效益以全垦整地最高为3 773.20元/hm2,种植沟整地次之为2 932.60元/hm2,打塘整地最低为1 971.90元/hm2.因此,在坡度较平缓、土层深厚的地块,宜采用全垦整地方法种植木豆;在坡度较大的地带,为最大程度地减少水土流失,宜采用打塘整地方法种植木豆.

  7. Initial Report on the Introduction Experiments of Cajanus cajan L. In Granite Erosion Area%花岗岩侵蚀区木豆引种试验初报

    Institute of Scientific and Technical Information of China (English)

    刘柏根; 张声林

    2006-01-01

    通过在花岗岩侵蚀区的引种试验表明,改良型木豆适应性强,在本地能较好地生长,具有鲜肥、薪柴产量高,再生能力强,保土效果好等特点,生长快,当年可见效,生态经济利用价值高,在燃料、饲料、保持水土等方面具有广阔的推广前景,是一种优良的水土保持植物.

  8. A fine species of greening hills and enriching the people——Cajanus cajan (Linn) Millsp%绿山富民的优良树种印度木豆

    Institute of Scientific and Technical Information of China (English)

    刘春晖; 何兴炳; 马利春; 徐小林

    2007-01-01

    印度木豆属于多年生灌木,其综合利用价值高,在中国南方半干旱地区适合引种栽培.本文针对印度木豆的生物学、生态学特性,栽培管理技术及综合利用价值进行综述,分析表明印度木豆全身是宝,经济用途广泛,同时在水土保持,生态恢复等方面具有重要地位.

  9. 不同品种的木豆在两种基质下的发芽出土试验%Germination Experiment of Varietied Cajanus cajan in Two Kinds of Matrix

    Institute of Scientific and Technical Information of China (English)

    谷勇; 邹恒芳; 周榕; 李正红; 杨贵成; 胡宾

    2004-01-01

    在营养土和红壤土基质条件下,通过对不同木豆品种在不同播种深度下的发芽出土试验研究结果表明:在营养土中品种间:ICPL87119与CK,ICPL87与ICP8094、云县对照样无显著性差异,ICPL87119与ICPL87、 ICP8094 呈显著性差异,最佳播种深度为2~5 cm;在红壤中品种间:云县对照样与各品种呈显著性差异,其余品种间无显著性差异,最佳播种深度为2 cm左右.

  10. 木豆叶水提物抗脑缺血缺氧损伤的作用研究%Studies on Water-soluble Extracts from Cajanus cajan Leaf against Hypoxic-ischemic Brain Damage

    Institute of Scientific and Technical Information of China (English)

    黄桂英; 廖雪珍; 廖惠芳; 邓素坚; 谭永恒; 周玖瑶

    2006-01-01

    目的探讨木豆叶水提物对脑缺血、缺氧损伤的保护作用.方法采用急性脑缺血再灌注模型法观察小鼠脑缺血后脑组织中超氧化物歧化酶(SOD)及过氧化脂质(MDA)的含量;采用急性脑缺血模型法观察大鼠脑缺血模型法观察大鼠脑缺血后脑组织的含水量、脑指数及脑毛细血管伊文思蓝的渗出量;采用小鼠断头法观察小鼠断头喘气时间.结果木豆叶水提物可显著降低急性脑缺血再灌注模型小鼠脑内MDA的含量,而SOD活力则显著提高,P<0.01;显著减少急性脑缺血模型大鼠脑组织的含水量及脑指数,P<0.05~0.01;显著减少急性脑缺血模型大鼠脑毛细血管伊文思蓝的渗出量,P<0.01;明显延长小鼠断头喘气时间,P<0.01.结论 木豆叶水提物对大鼠、小鼠脑缺血、缺氧损伤均有一定的保护作用.

  11. An Efficient Protocol for Micropropagation of Pigeon Pea(Cajanus cajan)via Adventitious Bud Regeneration%木豆不定芽再生途径的高效快繁体系

    Institute of Scientific and Technical Information of China (English)

    赵庆鹤; 崔博文; 文晓鹏

    2010-01-01

    以木豆成熟胚为外植体,通过探讨不同培养条件对不定芽的诱导、增殖及生根等能力的影响,以建立高效的快繁体系.结果表明:适合胚生长的培养基为MS附加0.5 mg/L BA和0.1 mg/L IBA;不定芽增殖培养基以EC6附加0.4mg/L BA与0.2 mg/L IBA效果最佳;生根培养基以MS附加0.1 mg/L IBA效果最好,生根率为82.4%,移栽成活率为85%.

  12. 白龙江河谷不同品种木豆生长比较%Comparative Study on Growth of Different Varieties of Cajanus cajan in Bailong River Valley

    Institute of Scientific and Technical Information of China (English)

    陈淑芬; 刘晓花; 汪海

    2012-01-01

    通过对6个引进木豆品种发芽时间、高生长量的观察,种子质量、产量及生长期与种源地指标的比较,初步筛选出了适宜于陇南白龙江河谷地区推广的品种,在科研生产中有一定参考价值.

  13. 岭南常用中药木豆叶中牡荆苷的HPLC含量测定%Determination of Vitexin in Cajanus cajan (L.) Millsp. by HPLC

    Institute of Scientific and Technical Information of China (English)

    李功营; 唐洪梅; 丘振文; 黄月纯; 张映娜

    2007-01-01

    目的 建立木豆叶中牡荆苷的含量测定方法.方法 色谱柱为Hypersiol ODS2 C18柱(250 mm×4.6mm,5 μm);流动相:0~20 min,甲醇:1%醋酸(25:75),20 min后,甲醇:1%醋酸(30:70);流速:1.0mL/min;检测波长:339 nm.结果 牡荆苷在0.104 4~0.522μg范围内线性关系良好,r=0.999 4,平均回收率为100.11%,RSD为0.63%.结论 该法简便,重复性、稳定性良好,可作为木豆叶中牡荆苷含量测定方法.

  14. ON Cajanus cajan's AND Dalbergia bulansae's Absor ptivitr OF 32p AND 86Rb%木豆、南岭黄檀对磷钾元素吸收效应的探讨

    Institute of Scientific and Technical Information of China (English)

    侯开卫; 陈玉德

    1983-01-01

    @@ 本文就木豆对磷钾(用铷代替钾)元素的吸收及其生长效应,磷素在木豆各器官的分布以及不同营养元素和不同含氮量对南岭黄檀吸收磷素的影响等方面进行了分析论述.

  15. 红壤施用石灰对木豆氮代谢的影响%Effect of Lime Application on Nitrogen Metabolism of Cajanus Cajan in the Acid Soil

    Institute of Scientific and Technical Information of China (English)

    程阳; 曹建华; 莫红英; 周莉; 舒丽

    2009-01-01

    大田试验结果表明:红壤土上施用适量的石灰能极显著增加木豆氮素营养水平,使氮代谢平衡向着有利于蛋白质积累的方向进行.在石灰处理浓度为100~150g株时,木豆的氮素水平达到较为理想的状况.随着石灰处理浓度的增加土壤脲酶,蛋白酶和硝酸还原酶的活性均呈现先增加后减少的趋势.

  16. 贵州花江峡谷木豆主要真菌病害调查%The Investigation of Major Fungal Diseases on Cajanus cajan in Huajiang Canyon Area in Guizhou

    Institute of Scientific and Technical Information of China (English)

    练启仙; 桑维钧; 杨茂发; 李小霞

    2009-01-01

    为了解真菌病害在木豆上的发生情况,采用实地调查、随机抽样调查并结合访问调查方法对贵州花江峡谷地区木豆主要真菌病害进行了研究.结果表明.木豆白粉病(Oidium sp.)、木豆褐斑病(Alternaria sp.)、木豆灰霉病(Botrytissp.)、木豆斑点病(Phyllosticta sp.)和木豆根腐病(Fusarium oxysporum)是木豆上发生的主要真菌病害,并对5种病害的发病症状和病原进行了描述.

  17. Identification of Coupling and Repulsion Phase DNA Marker Associated With an Allele of a Gene Conferring Host Plant Resistance to Pigeonpea sterility mosaic virus (PPSMV) in Pigeonpea (Cajanus cajan L. Millsp.).

    Science.gov (United States)

    Daspute, Abhijit; Fakrudin, B

    2015-03-01

    Pigeonpea Sterility Mosaic Disease (PSMD) is an important foliar disease caused by Pigeonpea sterility mosaic virus (PPSMV) which is transmitted by eriophyid mites (Aceria cajani Channabasavanna). In present study, a F2 mapping population comprising 325 individuals was developed by crossing PSMD susceptible genotype (Gullyal white) and PSMD resistant genotype (BSMR 736). We identified a set of 32 out of 300 short decamer random DNA markers that showed polymorphism between Gullyal white and BSMR 736 parents. Among them, eleven DNA markers showed polymorphism including coupling and repulsion phase type of polymorphism across the parents. Bulked Segregant Analysis (BSA), revealed that the DNA marker, IABTPPN7, produced a single coupling phase marker (IABTPPN7414) and a repulsion phase marker (IABTPPN7983) co-segregating with PSMD reaction. Screening of 325 F2 population using IABTPPN7 revealed that the repulsion phase marker, IABTPPN7983, was co-segregating with the PSMD responsive SV1 at a distance of 23.9 cM for Bidar PPSMV isolate. On the other hand, the coupling phase marker IABTPPN7414 did not show any linkage with PSMD resistance. Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7414 (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in F2 population. The repulsion phase marker, IABTPPN7983, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea. PMID:25774108

  18. Low-dose gamma irradiation induces water activity, leaf K+/Na+, glycine betaine, antioxidant enzyme activity and reduces lipid peroxidation and protease activity to enhance salt tolerance in pigeonpea [Cajanus cajan (L.) Millsp

    International Nuclear Information System (INIS)

    Soil salinity is a major constraint that limits legume productivity. The present study evaluates the physiological and biochemical basis of radiation affect on salt tolerance response of pigeonpea. Seed gamma irradiation, in general, at 1.92 (control), 5.86 (80 mM) and 8.02 dSm-1 (100 mM) soil electrical conductivity (NaCl stress), enhanced seedling establishment, plant growth, carbon metabolism and gas exchange characteristics such as net photosynthesis, stomatal conductance and transpiration rate. Further, an improvement in salt tolerance response of gamma irradiated (<10 Gy) pigeonpea was related to high seed water activity and leaf K+/Na+, glycine betaine, membrane stability index and enzymic antioxidant activity. (author)

  19. EFEITO DO PROCESSAMENTO SOBRE OS TEORES DE OXALATO E TANINO EM MAXIXE (CUCUMIS ANGURIA L., JILÓ (SOLANUM GILO, FEIJÃO VERDE (VIGNA UNGUICULATA (L. WALP E FEIJÃO ANDU (CAJANUS CAJAN (L. MILL SP

    Directory of Open Access Journals (Sweden)

    Clícia Maria de Jesus BENEVIDES

    2013-08-01

    Full Text Available Este trabalho objetivou avaliar o efeito do processamento térmico e do armazenamento em salmoura ácida, em função do tempo, nos teores de substâncias antinutricionais em hortaliças (maxixe e jiló e leguminosas (feijão verde e feijão andu comercializadas em Salvador- -BA. Os teores de oxalatos (ox e taninos (tan foram determinados nos vegetais in natura, submetidos ao branqueamento (100°C/3min e nos vegetais em salmoura ácida, hermeticamente fechados e armazenados, durante um período de seis meses. A determinação de oxalato foi realizada por permanganametria, utilizando solução de KMnO4 (0,002mol.L-1 e os taninos foram quantificados segundo o método de Folin Ciocalteau. Os valores médios (mg/100g encontrados nos vegetais in natura foram:maxixe (tan: 1,56±0,00 e ox: 1,44±0,00 e jiló (tan:3,46±0,04 e ox: 3,58±0,08. Com relação às leguminosas, os valores mé- dios (mg/100g foram: feijão verde (tan: 4,71±0,08 e ox: 4,92±0,03 e feijão andu (tan:6,05±0,02 e ox: 0,48±0,04. Houve redução média de 17,5% (ox e 10% (tan após o tratamento térmico e de 60,75% (ox e 89,2 % (tan após o armazenamento dos vegetais estudados, quando comparados com os teores dos mesmos na forma in natura. Conclui-se que os teores dos fatores antinutricionais diminuem após tratamento térmico e durante o armazenamento desses produtos em conservas.

  20. Avaliação de algumas propriedades funcionais das farinhas de tremoço doce (Lupinus albus e feijão guandu (Cajanus cajan (L Millsp e sua utilização na produção de fiambre Evaluation of some functional properties of lupin sweet flour (Lupinus albus and faba bean flour (Cajanus cajan (L Millsp and their utilization in cooked ham production

    Directory of Open Access Journals (Sweden)

    Fernando Castilho

    2010-03-01

    Full Text Available O aumento na demanda de alimentos no mundo tem motivado pesquisadores a buscarem fontes alternativas a fim de enriquecer nutricionalmente alimentos industrializados e torná-los viáveis economicamente às populações menos favorecidas. O tremoço doce e o feijão guandu são leguminosas com elevado conteúdo proteico e grande potencial de utilização no enriquecimento nutricional de alimentos industrializados, além de desempenharem funcionalidades. Os resultados obtidos neste trabalho permitiram observar que a farinha de tremoço doce é capaz de absorver de 172 a 186% do seu peso em água e de ~62% em óleo, enquanto que a farinha de feijão guandu absorve cerca de 100% de seu peso em água e 50% em óleo. Boa atividade e estabilidade foram observadas para as emulsões obtidas com estas farinhas, indicando potencial de aplicação na produção de embutidos. Objetivou-se neste trabalho, avaliar algumas propriedades funcionais das farinhas de tremoço doce e feijão guandu e sua utilização na fabricação de fiambres. Os fiambres foram elaborados no Laboratório de Controle de Qualidade de Alimentos da Faculdade de Ciências Farmacêuticas da Universidade Estadual Paulista (UNESP, na cidade de Araraquara, com o intuito de oferecer um alimento enriquecido nutricionalmente. Quando comparados ao fiambre elaborado com a adição de concentrado proteico de soja, estes fiambres não apresentaram diferenças significativas nos atributos sensoriais aparência, cor, aroma, textura e impressão global, embora tivessem pouca aceitabilidade sensorial.The increase in food demand worldwide has motivated researchers to seek alternative food crops to nutritionally enrich industrialized foods and make them economically viable to poorer populations. Sweet lupin and faba bean are high protein content legumes of great potential for nutritional enrichment of industrialized foods, besides performing a functional purpose. The results obtained in this work enabled to identify that sweet lupin flour is able to absorb 172 to 186% of its weight in water, and ~62% in oil, while faba bean flour absorbs ~100% of its weight in water, and ~50% in oil. The emulsions obtained with both flours showed good activity and stability, suggesting a good application potential in the production of sausages. In the present work, some functional properties of the sweet lupin and faba bean flours were analyzed, including use in the production of cooked ham. The cooked ham was elaborated in the Quality Control Laboratory of foods in the Pharmaceutical Sciences Faculty in the University of São Paulo State (UNESP in Araraquara (SP, Brazil, offering an enriched food nutritionally. When compared to cooked hams prepared with the addition of soy protein, the experimental hams presented no significant sensorial differences, such as appearance, color, flavor, texture and overall impression, however they had had little sensorial acceptability.

  1. Dicty_cDB: Contig-U04858-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available BBc0... 48 0.28 1 ( FI238693 ) CccaBb014-M24JF CccABb Cajanus cajan genomic clon... 48 0.28 1 ( EI078506 ) h...s clone CH240-164O7, WORKING DRAFT SEQUE... 44 4.3 1 ( FI262072 ) CccaBb057-M11JF CccABb Cajanus cajan genom...ic clon... 44 4.3 1 ( FI237281 ) CccaBb028-C7JF CccABb Cajanus cajan genomic clone... 44 4.3 1 ( FH621771 )

  2. Dicty_cDB: Contig-U00298-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ne R3-52G09, WOR... 46 0.97 1 ( FI263984 ) CccaBb039-L7JR CccABb Cajanus cajan genomic clone... 46 0.97 1 ( ... catenella mitochondrial cytb_1 gene f... 44 3.8 1 ( FI240547 ) CccaBb015-H8JR CccABb Cajanus... cajan genomic clone... 44 3.8 1 ( FI233294 ) CccaBb026-L11JF CccABb Cajanus cajan genomic clon.

  3. Dicty_cDB: Contig-U16249-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ... 38 8.4 2 ( FI231620 ) CccaBb001-K7JR CccABb Cajanus cajan genomic clone... 38... 8.5 2 ( AZ895509 ) RPCI-24-170F4.TJ RPCI-24 Mus musculus genomic clo... 36 9.6 2 ( FI237594 ) CccaBb016-K17JF CccABb Cajanus... cajan genomic clon... 38 9.6 2 ( FI273440 ) CccaBb050-E8JF CccABb Cajanus cajan genomic cl

  4. Dicty_cDB: Contig-U16195-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 16.TR Sheared DNA Trypanosoma bruc... 56 0.002 1 ( FI237470 ) CccaBb014-E21JF CccABb Cajanus... ( AY110562 ) Zea mays CL26590_1 mRNA sequence. 52 0.029 1 ( FI265286 ) CccaBb045-J21JF CccABb Cajanus cajan... genomic clon... 52 0.029 1 ( FI262825 ) CccaBb064-F23JF CccABb Cajanus cajan genomic clon... 52 0.029 1 ( F...I254950 ) CccaBb058-H16JR CccABb Cajanus cajan genomic clon... 52 0.029 1 ( ET713

  5. Dicty_cDB: Contig-U15353-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available P3AShA Triticum aestivu... 48 0.38 1 ( FI243267 ) CccaBb032-C18JR CccABb Cajanus cajan genomic clon... 34 1....1 2 ( FI234133 ) CccaBb023-N3JR CccABb Cajanus cajan genomic clone... 34 1.3 2 ( J02675 ) Rat prostatic sper...3.x1 NCI_CGAP_Pr35 Rattus norvegicus cDNA c... 46 1.5 1 ( FI247449 ) CccaBb026-C20JR CccABb Cajanus... cajan genomic clon... 34 1.7 2 ( FI261045 ) CccaBb058-E13JF CccABb Cajanus cajan genomic ...clon... 34 1.8 2 ( CR925829 ) C.jacchus DNA sequence from clone CH259-147B13. 44 1.9 3 ( FI231863 ) CccaBb001-L20JR CccABb Cajanus

  6. Dicty_cDB: Contig-U01776-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ESS *** f... 46 0.69 1 ( FI254975 ) CccaBb058-L22JR CccABb Cajanus cajan genomic clon... 46 0.69 1 ( FI21687...3 ) CccaBa060-B9TR CccABa Cajanus cajan genomic clone... 46 0.69 1 ( ET965986 ) CHO_OF424xl19f1.ab1 CHO_OF N

  7. Allelopathic Effects of Cajanus cajan L. On the Seed Germination and Seedling Growth of 6 Shrub & Grass Species%木豆化感作用对6种灌草种子萌发和幼苗生长的影响

    Institute of Scientific and Technical Information of China (English)

    许岳飞; 毕玉芬; 金晶炜

    2008-01-01

    以木豆为供体,黑麦草、高羊茅、非洲狗尾草、银合欢、大翼豆和白三叶为受体,运用室内培养皿法研究了木豆种子、木豆叶片浸提液对受体种子萌发和幼苗生长的影响.结果表明,木豆种子、叶片浸提液对6种受体植物种子萌发和幼苗生长均有化感作用,不同数量木豆种子和不同浓度的浸提液对受体植物化感作用强度不同,低浓度时较弱,高浓度时较强.木豆种子数量增加和浸提液的浓度提高均能降低种子的发芽率、胚轴和胚根长度、减少根毛的数量,其中胚根长度较其它指标更敏感,可能是衡量木豆化感作用的最敏感指标.不同植物对木豆化感作用的敏感程度不同,黑麦草、高羊茅、非洲狗尾草和白三叶化感作用较敏感,银合欢和大翼豆敏感性差.同时得出,木豆种子化感作用强于木豆叶片.

  8. 木豆种子萌发和幼苗生长过程中的芹菜素与木犀草素含量变化%Changes in Apigenin and Luteolin Contents during Seed Germination and Seedling Growth of Cajanus cajan (L.) Millsp.

    Institute of Scientific and Technical Information of China (English)

    付玉杰; 孔羽; 刘威; 佟美鸿; 祖元刚

    2006-01-01

    在木豆种子萌发和幼苗生长过程中,芹菜素与木犀草素含量呈波动型上升,各个器官中的木犀草素含量均高于芹菜素.种子萌发过程中,芹菜素与木犀草素最高含量分别是其在干种子的3.04和4.47倍.幼苗生长过程中,根中芹菜素和木犀草素含量高于茎和叶,芹菜素最高含量是茎和叶的1.5和1.6倍,木犀草素最高含量是茎和叶的4.4和4.2倍.二者在茎和叶中的含量及其变化趋势都基本上相似,平缓上升.

  9. Effect of Introducing Cajanus cajan (L.)Millsp.on Soil Fertility in Arid Hot Valley of Jinsha River%金沙江干热河谷地区引种印度木豆对土壤肥力的影响

    Institute of Scientific and Technical Information of China (English)

    李如丹; 郭华春; 董志渊; 谭文星; 傅志成; 陆晓屏

    2007-01-01

    在云南省永胜县金沙江干热河谷地区种植木豆,研究了种植木豆对土壤肥力的影响,结果表明:木豆耐旱、耐瘠,出苗后在较干旱情况下也可以正常生长,而且对土壤酸碱度有较广的适应性,作为先锋植物可在金沙江干热河谷生态恢复中发挥重要作用;种植木豆后的土壤全氮、碱解氮都有较大增加,表明种植木豆可以取到固氮,培肥地力的作用;同时种植木豆使土壤中全磷含量降低,而有效磷含量增加,说明木豆根释放的有机酸可分解土壤中的固态磷为速效磷,提高磷的利用效率,这些是木豆能够耐瘠薄的重要原因.

  10. 木豆叶提取物对H2O2诱导的H9c2细胞氧化损伤的保护作用及机制研究%Protective effects and mechanism of extract from Cajanus cajan leaves against H2O2-induced oxidative injury on H9c2 cardiomyoblasts

    Institute of Scientific and Technical Information of China (English)

    孙琳; 柴智; 张涛

    2016-01-01

    目的 研究木豆叶提取物(ECCL)对H2O2诱导的H9c2细胞氧化应激损伤的保护作用及其机制.方法 建立H2O2诱导的H9c2细胞氧化损伤模型,于H2O2刺激前加入PI3K信号通路阻断剂LY294002 (LY)预处理10 min,加入20、50 μg/mLECCL预处理24 h.MTT法检测细胞存活率,比色法测定上清液中乳酸脱氢酶(LDH)、丙二醛(MDA)水平和超氧化物歧化酶(SOD)的活性,Western blotting检测细胞内p-Akt、p-eNOS蛋白表达.结果 与模型组比较,ECCL可明显增加H9c2细胞H2O2损伤后细胞存活率(P<0.01),增加SOD活力,降低MDA、LDH水平,增加p-Akt和p-eNOS蛋白表达(P<0.05、0.01),LY可阻断ECCL对H9c2的上述作用.结论 ECCL能够减轻H2O2所致的H9c2细胞损伤,可能是通过激活PI3K通路促进其下游因子Akt和eNOS磷酸化发挥作用.

  11. Effect of extracting solution from Hemarthria altissima, Ageratum conyzoides and Zoysia matrella on germination and seedling growth of Merremia hederacea and Cajanus cajan%胜红蓟,牛鞭草和沟叶结缕草提取液对木豆、鱼黄草种子萌发和幼苗生长的影响

    Institute of Scientific and Technical Information of China (English)

    祁桂林; 陈平; 钟伟基

    2006-01-01

    用牛鞭草,胜红蓟和沟叶结缕草3种植物提取液处理鱼黄草和木豆品种3号、4号种子,测定种子发芽率、苗高、根长和苗鲜重.结果表明,牛鞭草提取液总体上对鱼黄草、木豆3号和4号产生了抑制种子发芽和幼苗生长的作用;胜红蓟提取液能明显抑制鱼黄草种子发芽和幼苗生长,显著降低木豆3号种子发芽率和苗高,高浓度胜红蓟提取液对木豆4号有抑制效应;沟叶结缕草提取液总体上对鱼黄草种子萌发和幼苗生长的抑制作用较小,对木豆3号稍有抑制效应,对木豆4号苗鲜重有显著抑制作用.

  12. 木豆叶芪类提取物对雌激素缺乏性大鼠骨质丢失的影响%Effects of the stilbene extracts from Cajanus cajan L. on ovariectomy-induced bone loss in rats

    Institute of Scientific and Technical Information of China (English)

    郑元元; 杨京; 陈迪华; 孙兰

    2007-01-01

    植物雌激素具有雌激素的抗骨丢失的作用,却没有雌激素的不良反应,因而受到广泛的重视。各国科学家都在积极寻找安全有效的、具有雌激素样作用的天然产物。已经发现的这类植物雌激素有异黄酮、木酚素、香豆素、二苯乙烯类等,在多种植物和中草药中均含有这些化合物。

  13. Study on the intercropping of Leucaena leucocephala with annual crops under dryland condition

    Energy Technology Data Exchange (ETDEWEB)

    Balasubramanian, T.N.; Robinson, J.G.; Ravikumar, V.

    1984-01-01

    Leucaena leucocephala was grown in paired rows with an interspace of 3 or 6 m between the two pairs of rows and was cut to a height of 50 or 100 cm. Sorghum, bajra (Pennisetum americanum) and red gram (Cajanus cajan) were grown either in the interspaces or in pure stands. Sorghum and bajra when grown both as pure stands and intercrops gave higher grain yields than seed yields of Cajanus cajan and the reduction in their yields as intercrops was also smaller. However, Leucaena leucocephala intercropped with Cajanus cajan in 6-m wide interspaces and cut to a height of 100 cm gave high fodder and seed yields and also produced more biomass in terms of fuel branch, roots and stump weight and gave the highest net returns. 5 references.

  14. Dicty_cDB: Contig-U01704-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .003 1 ( FI210818 ) CccaBa043-B3TR CccABa Cajanus cajan genomic clone... 42 0.003 3 ( AM462990 ) Vitis vinif....7 4 ( AM425165 ) Vitis vinifera contig VV78X176636.7, whole genome... 44 1.7 2 ( FI261308 ) CccaBb058-G10JF CccABb Cajanus...PROG... 38 2.1 5 ( FI272208 ) CccaBb036-K7JF CccABb Cajanus cajan genomic clone... 44 2.1 2 ( AC216609 ) Myo...bidopsis thali... 42 2.3 2 ( FI244820 ) CccaBb009-F19JF CccABb Cajanus cajan genomic clon... 44 2.3 2 ( BX93

  15. Dicty_cDB: Contig-U16005-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available clone Ch... 42 4.9 2 ( AM625921 ) Entamoeba dispar GSS, clone dispar122b06.q1k. 32 5.0 2 ( FI257161 ) CccaBb054-L5JR CccABb Cajanus...-1142D4, co... 38 5.0 6 ( FI232391 ) CccaBb002-M12JF CccABb Cajanus cajan genomic clon... 32 5.0 2 ( FI26811...2 ) CccaBb055-O23JR CccABb Cajanus cajan genomic clon... 32 5.0 2 ( FP090896 ) Pi

  16. Dicty_cDB: Contig-U03308-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 1 ( AC167093 ) Sorex araneus clone SA_Ba-207B12, WORKING DRAFT S... 48 0.32 1 ( FI239556 ) CccaBb022-P5JR CccABb Cajanus...3 1 ( AQ404889 ) HS_5044_B1_E02_SP6E RPCI-11 Human Male BAC Librar... 46 1.3 1 ( FI255788 ) CccaBb033-K4JR CccABb Cajanus... cajan genomic clone... 46 1.3 1 ( FI224926 ) CccaBa056-B8TV CccABa Cajanus cajan genomic clone

  17. Dicty_cDB: Contig-U01735-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available purpuratus clone R3-3047L05, W... 52 0.011 1 ( FI240593 ) CccaBb015-P24JR CccABb Cajanus...9 ) Gm_ISb001_018_B09R ISU Soybean BAC Library (pBelo... 44 2.8 1 ( FI251731 ) CccaBb028-F20JR CccABb Cajanus... cajan genomic clon... 44 2.8 1 ( FI235251 ) CccaBb024-K20JF CccABb Cajanus cajan genomic clon... 44 2.8 1

  18. Dicty_cDB: Contig-U02610-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 52 0.028 1 ( BH126744 ) BARC-Satt578 Size-selected soybean genomic Glycin... 52 0.028 1 ( FI249972 ) CccaBb012-L20JR CccABb Cajanus... cajan genomic clon... 52 0.028 1 ( FI238074 ) CccaBb015-C24JR CccABb Cajanus...z... 50 0.11 1 ( FI262698 ) CccaBb041-L13JR CccABb Cajanus cajan genomic clon... 50 0.11 1 ( CT419388 ) Sus

  19. Dicty_cDB: Contig-U02161-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .011 15 ( AE014828 ) Plasmodium falciparum 3D7 chromosome 14 section 1... 34 0.011 15 ( FI247919 ) CccaBb008-O16JF CccABb Cajanus...n Genomic Spe... 46 3.2 1 ( FI240260 ) CccaBb005-D5JF CccABb Cajanus cajan genomic clone... 46 3.2 1 ( FI232...251 ) CccaBb002-C15JF CccABb Cajanus cajan genomic clon... 46 3.2 1 ( FI203718 ) CccaBa026-A23TV CccABa Cajanus

  20. Dicty_cDB: Contig-U05485-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 3 ( CZ514269 ) GMW2-40K24a.b1 GMW2 Glycine max genomic, genomic ... 36 0.081 2 ( FI243191 ) CccaBb009-E6JR CccABb Cajanus... cajan genomic clone... 40 0.082 2 ( FI267034 ) CccaBb063-C20JF CccABb Cajanus...nomic clo... 44 1.6 1 ( FI212785 ) CccaBa035-E23TR CccABa Cajanus cajan genomic clon... 44 1.6 1 ( FI040128

  1. Obtención de alimentos e ingredientes con propiedades antihipertensivas a partir de leguminosas germinadas y fermentadas

    OpenAIRE

    Limón Garduza, Rocío Ivonne

    2015-01-01

    Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Química Agrícola. Fecha de lectura: 14-05-2015 La hipertensión es una patología que afecta aproximadamente a un tercio de la población adulta y constituye uno de los factores de riesgo de las enfermedades cardiovasculares, lo que supone un grave problema de salud pública mundial. La prevención primaria basada en el ejercicio físico y una dieta saludable representa una estrategia segura,...

  2. Dicty_cDB: Contig-U01777-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available PROGRE... 44 9.1 1 ( AZ454843 ) 1M0256P23R Mouse 10kb plasmid UUGC1M library Mus ... 44 9.1 1 ( FI193750 ) CccaBa014-J13TR CccABa Caj...anus cajan genomic clon... 44 9.1 1 ( ER484768 ) 1093015291860 Global-Ocean-Samplin

  3. Dicty_cDB: VSI320 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available I706. 62 3e-18 2 ( CU681840 ) Zebrafish DNA sequence from clone CH73-285P12 in ... 36 0.003 2 ( FI242080 ) CccaBb006-G7JR CccABb Caja...nus cajan genomic clone... 42 0.004 2 ( FG087126 ) CMRC-

  4. Dicty_cDB: Contig-U04794-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 208071 ) CccaBa008-B18TR CccABa Cajanus cajan genomic clon... 46 1.1 1 ( EK307042 ) 1095462384214 Global-Oce...67 ) Homo sapiens chromosome 17 clone RP11-339D15 map ... 44 4.3 1 ( FI237139 ) CccaBb031-G17JR CccABb Cajanus

  5. Dicty_cDB: Contig-U04992-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CccaBa025-L14TR CccABa Cajanus cajan genomic clon... 44 5.4 1 ( EB237306 ) PEG003...T SEQUE... 44 5.4 1 ( AC013659 ) Homo sapiens clone RP11-20A13, WORKING DRAFT SEQU... 44 5.4 1 ( FI196225 )

  6. Dicty_cDB: Contig-U15230-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available WBb0099E05.f GM_WBb Glycine max genomic clone ... 34 0.19 2 ( FI258917 ) CccaBb053-C16JF CccABb Cajanus caja...... 36 0.35 2 ( FI273451 ) CccaBb050-G10JF CccABb Cajanus cajan genomic clon... 36 0.36 2 ( AM467491 ) Vitis

  7. Dicty_cDB: Contig-U15662-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available AZ349261 ) 1M0086M07F Mouse 10kb plasmid UUGC1M library Mus ... 46 6.8 1 ( FI215330 ) CccaBa054-C19TR CccABa Cajanus... cajan genomic clon... 46 6.8 1 ( FI208250 ) CccaBa006-H3TV CccABa Cajanus

  8. Dicty_cDB: Contig-U01815-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available nianus cD... 44 3.2 1 ( ES393277 ) MUS13-K08.y1d-s SHGC-MUS Mytilus californianus c...-L2JF CccABb Cajanus cajan genomic clone... 44 3.2 1 ( ES393488 ) MUS13-G14.y1d-s SHGC-MUS Mytilus califor

  9. Detection of Legume Protease Inhibitors by the Gel-X-ray Film Contact Print Technique

    Science.gov (United States)

    Mulimani, Veerappa H.; Sudheendra, Kulkarni; Giri, Ashok P.

    2002-01-01

    Redgram (Cajanus cajan L.) extracts have been analyzed for the protease inhibitors using a new, sensitive, simple, and rapid method for detection of electrophoretically separated protease inhibitors. The detection involves equilibrating the gel successively in the protease assay buffer and protease solution, rinsing the gel in assay buffer, and…

  10. Avaliação quantitativa e qualitativa da fitomassa de leguminosas para uso como cobertura de solo Quantitative and qualitative evaluation of legumes as soil cover

    Directory of Open Access Journals (Sweden)

    João Tavares Nascimento

    2004-06-01

    Full Text Available Para uso como adubo verde em um experimento em Alagoinha-PB, avaliaram-se doze espécies de leguminosas: crotalária (Crotalaria juncea L, guandu (Cajanus cajan L, guandu anão (Cajanus cajan (L Millsp, calopogônio (Calopogonium mucunoides L, feijão-de-porco (Canavalia ensiformis L, lab-lab (Dolichos lab lab L, kudzu tropical (Pueraria phaseoloides L, siratro (Macroptilium atropurpureum L, leucena (Leucaena leucocephala L, cunhã (Clitoria ternatea L, mucuna preta (Styzolobium aterrimum L, e mucuna cinza (Styzolobium cinereum Piper e Tracy, no DBC com 12 tratamentos, em parcelas de 20m2, com três repetições. Constataram-se as maiores produções de fitomassa para leucena, guandu, mucuna preta, kudzu tropical, feijão-de-porco e cunhã, enquanto que para a crotalária, a menor produção. A fitomassa do kudzu tropical mostrou-se com melhor qualidade para a incorporação.Twelve species of tropical legumes (Crotalaria juncea L, Cajanus cajan L, Cajanus cajan (L Millsp, Calopogonium mucunoides L, Canavalia ensiformis L, Dolichos lab lab L, Pueraria phaseoloides L, Macroptilium atropurpureum L, Leucaena leucocephala L, Clitoria ternatea L, Styzolobium aterrimum L, Styzolobium cinereum Piper and Tracy was evaluated for use as green manure or cover crops in an experiment in Alagoinha-PB, Brazil. The used experimental design was a ramdomized complete block design, with 12 treatments in plots of 20m2, with three repetitions .The largest biomass quantities were produced for Leucaena leucocephala, Cajanus cajan, Styzolobium aterrimum, Pueraria phaseoloides, Canavalia ensiformis and Clitoria ternatea, while Crotalaria juncea, produced the smallest biomass. The biomass of Pueraria phaseoloides, was shown with better quality for the incorporation.

  11. A pigeonpea gene confers resistance to Asian soybean rust in soybean.

    Science.gov (United States)

    Kawashima, Cintia G; Guimarães, Gustavo Augusto; Nogueira, Sônia Regina; MacLean, Dan; Cook, Doug R; Steuernagel, Burkhard; Baek, Jongmin; Bouyioukos, Costas; Melo, Bernardo do V A; Tristão, Gustavo; de Oliveira, Jamile Camargos; Rauscher, Gilda; Mittal, Shipra; Panichelli, Lisa; Bacot, Karen; Johnson, Ebony; Iyer, Geeta; Tabor, Girma; Wulff, Brande B H; Ward, Eric; Rairdan, Gregory J; Broglie, Karen E; Wu, Gusui; van Esse, H Peter; Jones, Jonathan D G; Brommonschenkel, Sérgio H

    2016-06-01

    Asian soybean rust (ASR), caused by the fungus Phakopsora pachyrhizi, is one of the most economically important crop diseases, but is only treatable with fungicides, which are becoming less effective owing to the emergence of fungicide resistance. There are no commercial soybean cultivars with durable resistance to P. pachyrhizi, and although soybean resistance loci have been mapped, no resistance genes have been cloned. We report the cloning of a P. pachyrhizi resistance gene CcRpp1 (Cajanus cajan Resistance against Phakopsora pachyrhizi 1) from pigeonpea (Cajanus cajan) and show that CcRpp1 confers full resistance to P. pachyrhizi in soybean. Our findings show that legume species related to soybean such as pigeonpea, cowpea, common bean and others could provide a valuable and diverse pool of resistance traits for crop improvement. PMID:27111723

  12. Susceptibility of leguminous green manure species to Rhizoctonia solani and Sclerotium rolfsii

    Directory of Open Access Journals (Sweden)

    Trazilbo José de Paula Júnior

    2011-12-01

    Full Text Available We studied the susceptibility of species used as green manure in common bean fields to root rot (Rhizoctonia solani and southern blight (Sclerotium rolfsii. Seeds of Crotalaria breviflora, Canavalia ensiformis, Cajanus cajan, Dolichos lablab, Stizolobium cinereum, S. aterrimum, and the bean cvs. "Pérola", "Valente" and "Carnaval" were sown in soil infested by either R. solani AG-4 or S. rolfsii in greenhouse. The emergence of D. lablab seedlings in soil infested by R. solani dropped to 62%. C. breviflora, C. ensiformis and cv. "Valente" presented the lowest root rot severity. The pathogen S. rolfsii drastically reduced seedling emergence in all species; no C. cajan and S. cinereum seedling emerged. All plant species presented high southern blight severity. We conclude that leguminous crops are not suitable as green manure for areas of bean cultivation with high R. solani and S. rolfsii populations.

  13. Efficacy of nanostructured silica as a stored pulse protector against the infestation of bruchid beetle, Callosobruchus maculatus (Coleoptera: Bruchidae)

    Science.gov (United States)

    Arumugam, Ganesh; Velayutham, Veeramani; Shanmugavel, Sakthivelkumar; Sundaram, Janarthanan

    2016-03-01

    The treatment of hydrophobic silica nanoparticles (SNPs) with the pulse seeds of Cajanus cajan, Macrotyloma uniflorum, Vigna mungo, Vigna radiata, Cicer arietinum and Vigna unguiculata against the infestation of stored pulse beetle, Callosobruchus maculatus revealed a significant reduction in oviposition, adult emergence and seed damage potential. There was a complete retardation of growth of this beetle in the treated seeds of C. cajan. SNP-treated seeds of these six varieties of pulses revealed no effect on the growth of seeds as revealed by seed germination, growth rate of root and shoot. Similarly, the soil microflora measured in terms of colony forming units was not affected by silica nanoparticles upon its treatment with pulse seeds. The results of this study thus clearly demonstrated the useful nature of silica nanoparticles as seed protecting agent for the control of C. maculatus.

  14. 广西平果县石漠化治理模式%Management Model for Rocky Desertification in Pingguo County, Guangxi

    Institute of Scientific and Technical Information of China (English)

    黄惠

    2005-01-01

    广西平果县是石山区,在石漠化治理过程中,总结出了任豆(Zenia insignis)×丛竹生、任豆×木豆(Cajanus cajan)、任豆×金银花(Lonicera japonica)、任豆×银合欢(Leuceana leucocephala)等多个经营模式,提高了造林绿化的效率.

  15. 印度国际木豆技术培训团学习考察报告%A Report on International Pigeonpea Technology Training Group in India

    Institute of Scientific and Technical Information of China (English)

    谢贵水

    2003-01-01

    简要介绍了木豆(Cajanus cajan Millspaugh,C.bicolor DC.)和国际半干旱热带作物研究所(ICRISAT)的一些基本情况,以及该所在木豆研究方面的最新进展,并对中国木豆产业的发展提出了一些建议.

  16. 木豆杂交种生产体系构建及新品种选育研究

    Institute of Scientific and Technical Information of China (English)

    李正红; K.S.Saxena

    2011-01-01

    木豆(Cajanus cajan)系蝶形花多年生常绿灌木,具木本粮食、蔬菜、饲料、放养紫胶虫生产紫胶、干籽粒加工小食品及酒等综合利用价值,叶是治疗股骨头坏死的有效药物.

  17. 木豆2号的异交特性研究

    Institute of Scientific and Technical Information of China (English)

    庞雯; 韦广天; 等

    2002-01-01

    木豆(Cajanus cajan)是闭花授粉作物,但由于昆虫的传粉,会产生自然异交.木豆2号的自然异交率在0~62.5%之间,平均异交率为29.5%.进行木豆种子繁殖时,必须采取有效的隔离保纯措施.

  18. Dicty_cDB: Contig-U05668-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 166506 ) Bos taurus clone CH240-76C12, WORKING DRAFT SEQUE... 46 1.3 1 ( FI195395 ) CccaBa020-H20TR CccABa Cajanus...8_B1_D05_T7A CIT Approved Human Genomic Spe... 44 5.1 1 ( FI213479 ) CccaBa064-E15TR CccABa Cajanus cajan ge

  19. DEVELOPMENT AND PERFORMANCE EVALUATION OF TRACTOR FRONT MOUNTED PIGEON PEA STEM CUTTER

    OpenAIRE

    Atul R. Dange; S.K.Thakare

    2010-01-01

    Pigeon pea or tur (Cajanus cajan L. Mills.) is one of the important pulse crops of India and ranks second to chickpea in area and production. Traditionally the harvesting of pigeon pea is done manually by sickle, which demands considerable amount of labour, drudgery, time and cost to harvest, which reflects on total production cost of the crop. In view of this a tractor operated front mounted pigeon pea stem cutter was developed and being front mounted implement it facilitated better visibil...

  20. Digestão dos carboidratos de alimentos volumosos em eqüinos Digestion of carbohydrates of forages in horses

    OpenAIRE

    Eliane da Silva Morgado; Fernando Queiroz de Almeida; Vinícius Pimentel Silva; Augusto Vidal da Costa Gomes; Leandro Galzerano; Henrique Torres Ventura; Liziana Maria Rodrigues

    2009-01-01

    Neste estudo, foram realizados dois ensaios com os objetivos de avaliar o fracionamento dos carboidratos de alimentos volumosos e estimar a digestibilidade desses nutrientes em dois ensaios de digestão com eqüinos. No ensaio 1, foram utilizados cinco eqüinos em delineamento de blocos casualizados, com sete tratamentos - fenos de alfafa (Medicago sativa); amendoim forrageiro (Arachis pintoi); desmódio (Desmodium ovalifolium); guandu (Cajanus cajan); macrotiloma (Macrotyloma axillare); estilosa...

  1. Survey on pigeon pea production systems, utilization and marketing in semi-arid lands of Kenya

    OpenAIRE

    Baudoin J.P.; Le Roi A.; Mergeai G.; Kimani P.; Mwang'ombe A.; Olubayo F; Smith C.; Audi P.

    2001-01-01

    In order to assess the status of pigeonpea (Cajanus cajan (L.) Millsp.) production in Kenya, two surveys were carried out in Makueni and Mbeere Districts in areas representative of the main agro-ecological pigeonpea producing zone of the country : (Mid-altitude ASAL). Participatory Rural Appraisal (PRA) approach was chosen as research method and was completed by household interviews based on a semi-structured questionnaire. The main points developed are the presentation of the different farmi...

  2. Microstructure of Black, Green and Red Gram

    OpenAIRE

    Joseph, Enamuthu; Crites, Shelly G.; Swanson, Barry G.

    1993-01-01

    The three most commonly consumed legumes (grams or pulses) in India, black gram (Vigna mungo (L.) Hepper), or urd , green gram (Vigna radiata (L.) Wilczek) or mung, and red gram (Cajanus cajan (L.) Millsp.) or tur, were examined by scanning electron microscopy . Seed coat and internal features were examined to differentiate these legumes from common beans (Phaseolus vulgaris) , adzuki beans (Vigna angu/aris) and lentils (Lens culinaris). Cross-sections of the seed coats of black, green and re...

  3. Productive performance of naked neck chickens that were fed leaf meal shrubs

    OpenAIRE

    Santos M Herrera G.; Aslam Díaz C.

    2016-01-01

    ABSTRACT Objective. To compare the productive performance of naked neck chickens (phases of initiation, growth and final) that were fed meals Gliricidia sepium, Cajanus cajan and Morus alba leaves. Materials and methods. 192 chickens, 1-84 days of age were distributed in a randomized block design with three experimental groups (5% of shrub in the diet), 48 animals/ group, eight replicates/ treatment, six animals/ reply and three animals/ sex in each replicate were used. The control group con...

  4. Plant residues: short term effect on sulphate, borate, zinc and copper adsorption by an acid oxisol

    OpenAIRE

    2003-01-01

    Laboratory experiments were carried out to examine the effects of plant residues on Cu, Zn, B and S adsorption by an acidic oxisol. The plant residues were: black oats (Avena strigosa), oil seed radish(Raphanus sativus), velvet beans (Stizolobium cinereum), and pigeon pea (Cajanus cajan) collected at flowering stage. Plant residues increased Cu and Zn adsorptions and decreased B and S adsorptions. The results indicated that for short term effect plant residues decreased the availabilities of ...

  5. Isolation of Aspergillus flavus from stored food commodities and Thymus vulgaris (L.) essential oil used as a safe plant based preservative

    OpenAIRE

    Atul Kumar Singh; Chandrabhan Seniya; Shriram Prasad

    2009-01-01

    Grain samples of Cicer arietinum (Chickpea), Zea mays (Maize), Cajanus cajan (Pigeon pea), Hordeum vulgare (Barley), Oryza sativa (Rice) and Sorghum vulgare (Millet) were procured from various retailers of market were subjected to their mould profile. During mycoflora analysis, 1297 fungal isolates were recorded from the food commodities. The least number of fungal isolates (189) were detected from H. vulgare while highest (244) from Z. mays. The genus Aspergillus was found to be most dominan...

  6. In situ caecal degradation of roughages in horses Degradação cecal in situ de alimentos volumosos em equinos

    OpenAIRE

    Vinícius Pimentel Silva; Fernando Queiroz de Almeida; Eliane da Silva Morgado; Liziana Maria Rodrigues; Tiago Marques dos Santos; Henrique Torres Ventura

    2010-01-01

    The present study was carried out to evaluate the in situ degradation of dry matter (DM), neutral detergent fiber (NDF) and crude protein (CP) in roughages by the in situ caecal digestion technique in horses. The roughages evaluated were: Lucerne hay (Medicago sativa), peanut (Arachis pintoi cv. Amarillo), desmodio(Desmodium ovalifolium), stylo (Stylosanthes guianensis cv. Mineirão), pigeon pea (Cajanus cajan), lime-yellow pea (Macrotyloma axillare) and coastcross hay (Cynodon dactylon cv. co...

  7. Digestibilidade dos nutrientes de alimentos volumosos determinada pela técnica dos sacos móveis em eqüinos Nutrient digestibility of forage feed determined using mobile bag technique in horses

    OpenAIRE

    Vinícius Pimentel Silva; Fernando Queiroz de Almeida; Eliane da Silva Morgado; Almira Biazon França; Henrique Torres Ventura; Liziana Maria Rodrigues

    2009-01-01

    Objetivou-se estimar a digestibilidade de nutrientes de forrageiras em eqüinos utilizando-se a técnica de sacos de náilon móveis. Foram avaliados alfafa (Medicago sativa), amendoim forrageiro (Arachis pintoi), desmódio (Desmodium ovalifolium), estilosantes (Stylosanthes guianensis), guandu (Cajanus cajan), macrotiloma (Macrotyloma axillare) e capim-coastcross (Cynodon dactylon cv. coastcross). O delineamento foi em blocos inteiramente casualizados com sete alimentos e cinco blocos (animais). ...

  8. Diversity of root nodule bacteria from leguminous crops

    OpenAIRE

    Agrawal Pooja; Shukla Shruti

    2016-01-01

    In the present study, a total of 353 nodule-associated bacteria were isolated from 220 legume plant samples belonging to Cicer arietinum (85), Glycine max (74), Vigna radiata (21) and Cajanus cajan (40). A total of 224 bacteria were identified as fast-growing Rhizobium spp. on the basis of differential staining (Gram staining and carbol fuchsin staining) and biochemical tests. All the isolates were tested for indole acetic acid production (IAA), phosphate s...

  9. Influence of nitrogen fertilization and green manure on the economic feasibility of no-tilled wheat in the Cerrado

    Directory of Open Access Journals (Sweden)

    Douglas de Castilho Gitti

    2012-04-01

    Full Text Available The search for higher profitability in wheat crop with cost reduction technologies that may promote sustainability is an important matter in Brazilian agriculture. This study evaluated the profitability of no-tilled wheat, reducing nitrogen topdressing doses with the cultivation of green manure before the wheat crop. The experiment was carried out in Selvíria (MS, Brazil, in 2009/10. The experiment was arranged in a randomized block design with 36 treatments in splitplots and four replicates. The plots were formed by six types of green manure: Cajanus cajan L. BRS Mandarin, Crotalaria juncea L., Pennisetum americanum L. BRS 1501, fallow area and mixed cropping of Pennisetum americanum L. + Cajanus cajan L. and Pennisetum americanum L. + crotalaria which provided straw for no-tilled wheat in the winter, following the rice crop in the summer. The subplots were formed by six levels of topdressing nitrogen (0, 25, 50, 75, 100 and 125 kg N ha-1 using urea as a nitrogen source. The wheat grown after green manure in the previous winter crop, with no nitrogen topdressing and a rate of 25 kg ha-1 N, had more frequently production costs above the gross income. Wheat production cost after the mixed cropping Pennisetum americanum L. + Cajanus cajan L. and Pennisetum americanum L. + Crotalaria juncea L. from the previous winter crop, combined with nitrogen rates of 50 and 75 kg N ha-1, provided better profitability compared with the other green manures evaluated.

  10. Pore size distribution of a deeply excavated Oxisol after 19 years reclamation

    Science.gov (United States)

    dos Santos Batista Bonini, Carolina; de Cássia Marchini, Débora; Alves, Marlene Cristina; García de Arruda, Otton; Paz-Ferreiro, Jorge

    2013-04-01

    Digging of the local soil and using it as a raw material for construction purposes has been identified as a non-negligible source of land degradation. Techniques aimed at soil profile reconstruction and ecological restoration of soils truncated by mechanical excavation using heavy machinery have been investigated Both, total soil porosity and pore size distribution are important properties for soil management as well as for assessing the recovery of soil function after land degradation. In this way, macropores are responsible for aeration, whereas water storage depends on soil meso- and micropores in the soil and the optimal pore-size distribution is also an indicator of soil quality. We investigated the changes in the pore size distribution of a soil that was beheaded to extract raw materials after a 19 year period of reclamation, which involved the use of green manures, gypsum and pasture for the purpose of profile recovery. The studied area is located in Mato Grosso do Sul State, Brzil. A field trial was performed following a completely randomized experimental design with seven treatments and four replications. Starting 1992, the initial treatments were: 1) control (tilled bare soil), 2)Stizolobium aterrium, 3)Cajanus cajan, 4)lime+S. aterrimum, 5) lime+C. cajan, 6) lime + gypsum + S. aterrimum, 7) lime + gypsum+C. cajan. In 1994, all treatments with C. cajan were replaced by Canavalia ensiformis and in 1999, Brachiaria decumbens was implanted in all the experimental plots. Data from vegetated treatments were compared with bare soil (control) and native vegetation (Savannah). Soil samples were collected in 2011 at the 0.00-0.10, 0.10-0.20, and 0.20-0.40 m depths. Treatment differences were assessed by analysis of variance, following the Scott-Knott test (5%) of probability to compare averages. Macroporosity of the 0.00-0.10 m top layer was above the 0.10 m3m-3 threshold considered as critical for plant growth. On the 0.10-0.20 m layer only treatments with C

  11. Structural quality of on Oxisol in recovery for 18 years

    Science.gov (United States)

    dos Santos Batista Bonini, C.; Alves, M. C.; Marchini, D. C.; Garcia de Arruda, O.; Nilce Souto Filho, S.

    2012-04-01

    Incorrect use of soil and large buildings construction in rural areas are causing changes to it, making them less productive and thus increasing the degraded areas. Techniques aimed at ecological restoration of degraded soils have been investigated. In this sense we investigated the positive changes in the structural quality of a soil that was beheaded in human intervention techniques for recovery for 18 years, having been used green manures, gypsum and pasture. The studied area is located in Mato Grosso do Sul, Brazil. The experimental design was a completely randomized with seven treatments and four replications. The treatments were: control (tilled soil without culture); Stizolobium aterrium; Cajanus cajan; lime+S. aterrimum; lime+C. cajan; lime+gypsum+S. aterrimum; lime+gypsum+C. cajan. In 1994, all treatments with C. cajan were replaced by Canavalia ensiformis and in 1999, Brachiaria decumbens was implanted in all treatments. Data from vegetated treatments were compared with the control bare soil and native vegetation (savannah). We evaluated the distribution and aggregate stability in water, soil samples were collected in 2010 in the depths: 0.00-0.10; 0.10-0.20 and 0,20-0.40 m. The results were analyzed by analysis of variance, following Scott-Knott test (5%) of probability to compare averages. Evaluating the results is noted that in the depth of 0.00-0.10 m, the control bare soil and savannah soil had lower and higher DMP, respectively. All recovery treatments were DMP greater than found for the bare soil control. Treatments: S. aterrimum, lime + gypsum + C. cajan and lime + gypsum + S. aterrimum and the savannah control were similar in the depth of 0.00-0.10 m. All of the recovery treatment in the depth from 0.00-0.10 m with values is close to the native vegetation of the savannah. Depths of 0.10-0.20 and 0.20-0.40 m results obtained for DMP treatments in recovery are similar to the bare soil, except for treatments with S. aterrimum and lime + gypsum + S

  12. Environmental and agricultural benefits of a management system designed for sandy loam soils of the humid tropics Benefícios ambientais e agronômicos de um agrossistema definido para solos de textura franco arenosa do trópico úmido

    Directory of Open Access Journals (Sweden)

    Alana das Chagas Ferreira Aguiar

    2009-10-01

    Full Text Available A sustainable management of soils with low natural fertility on family farms in the humid tropics is a great challenge and overcoming it would be an enormous benefit for the environment and the farmers. The objective of this study was to assess the environmental and agronomic benefits of alley cropping, based on the evaluation of C sequestration, soil quality indicators, and corn yields. Combinations of four legumes were used in alley cropping systems in the following treatments: Clitoria fairchildiana + Cajanus cajan; Acacia mangium + Cajanus cajan; Leucaena leucocephala + Cajanus cajan; Clitoria fairchildiana + Leucaena leucocephala; Leucaena leucocephala + Acacia mangium and a control. Corn was used as a cash crop. The C content was determined in the different compartments of soil organic matter, CEC, available P, base saturation, percentage of water saturation, the period of the root hospitality factor below the critical level and corn yield. It was concluded that alley cropping could substitute the slash and burn system in the humid tropics. The main environmental benefit of alley cropping is the maintenance of a dynamic equilibrium between C input and output that could sustain up to 10 Mg ha-1 of C in the litter layer, decreasing atmospheric CO2 levels. Alley cropping is also beneficial from the agricultural point of view, because it increases base saturation and decreases physical resistance to root penetration in the soil layer 0 - 10 cm, which ensures the increase and sustainability of corn yield.O manejo sustentável dos solos de baixa fertilidade natural na agricultura familiar do trópico tem sido um grande desafio, que, se vencido, resultará em vantagens para o ambiente e para os agricultores. Este trabalho foi realizado com o objetivo de avaliar os benefícios ambiental e agronômico de um cultivo em aleias, por meio da determinação do C sequestrado, dos indicadores da qualidade do solo e da produtividade da cultura do milho

  13. Ecological Restoration Effect on Interplant of Tamarindus indica Artificial Forest in Yuanmou Dry and Hot Valley%元谋干热河谷罗望子人工林间作生态修复效应

    Institute of Scientific and Technical Information of China (English)

    李纪潮; 潘志贤; 岳学文; 何光熊; 史亮涛; 纪中华

    2014-01-01

    在建林15年的罗望子人工林内间作木豆(Cajanus cajan)、柱花草(Stylosanthes guianensias)、象草(Pennisetum purpureum)等灌草植物,构建新的生态修复模式.结果表明生态系统的物理性状、化学性状、经济性能均得到明显的提升.但豆科的木豆、柱花草效果更佳.

  14. 木豆简介

    Institute of Scientific and Technical Information of China (English)

    胡家蓬

    2000-01-01

    @@木豆〔Cajanus cajan (L.)Millspaugh〕是热带和亚热带国家一种主要的豆类。由于它的株形灌木状,豆荚结在树冠的枝条上,称之为木豆。因为鸽子爱吃此豆,又有鸽豆之称。它的豆粒象黄豆,我国南方俗称大豆树。

  15. 元谋干热河谷木豆引种栽培试验初报%Applied Trial of Cajanus's Introducing a Fine Varity and Growing In Yuanmou Dry and Hot Valley

    Institute of Scientific and Technical Information of China (English)

    杨艳鲜; 纪中华; 杨顺林; 冯光恒

    2003-01-01

    @@ 木豆(Cajanus cajan(L.)Millspaugh),又名树豆、千年豆、三叶豆、鸽子豆、蓉豆、柳豆、扭豆、黄豆树等,英文名Pigeonpea或Red Gram.原产于印度,是印度、东非和加勒比地区的重要经济树种,是迄今为止唯一的一种木本食用豆科作物.

  16. 木豆种质资源遗传多样性研究中的AFLP技术优化及引物筛选%Optimization of AFLP Fingerprinting and Screening of Primer Pairs in Genetic Diversity Analysis of Pigeonpea Germplasm

    Institute of Scientific and Technical Information of China (English)

    闫龙; 关建平; 宗绪晓

    2004-01-01

    以来自国际半干旱热带作物研究所(ICRISAT)的2份栽培木豆( Cajanus cajan)和1份野生木豆(Cajanus scarabaeoides)资源为试材,将AFLP技术体系在木豆资源研究上进行优化,并筛选出20对多态性高的AFLP引物组合,推荐用于将来的木豆种质遗传多样性分析.

  17. Evaluación de seis abonos verdes en un vertisol ústico en condiciones del Valle del Cauca

    Directory of Open Access Journals (Sweden)

    Bonilla C. C. R.

    2004-12-01

    Full Text Available En el Centro Experimental de la Universidad Nacional de Colombia-Sede Palmira, en un diseño de bloques completos al azar, se evaluaron seis tratamientos con tres repeticiones. (Cajanus cajan guandul arbóreo y arbustivo; Canavalia ensiformis, fríjol Canavalia; Cynodon niemfuensis, pasto estrella; Zea mays, Maíz y Cucúrbita moschata, zapallo. El pasto estrella y el zapallo fueron cultivos previos al ensayo; los otros materiales se sembraron por semilla a 50 cm en cuadro y se incorporaron al suelo a los 150 días cuando se cosechó el maíz (choclo. Maíz y canavalia aportaron mayor biomasa al suelo (10.068 y 9.748 kg/ha, respectivamente. C. cajan arbóreo y C. ensiformis aportaron las mayores cantidades de nitrógeno (254 y 213 kg/ha. El maíz y el pasto estrella, por sus altos contenidos de lignina, se descompusieron más lentamente. En casi todos los tratamientos se presentó incremento de la población microbiana, predominando la población de bacterias, seguida por hongos, fijadores de nitrógeno de vida libre y luego la población de actinomicetos. ABSTRACT Evaluation of six green manure materials in a Ustic Vertisol Soil of Cauca Valley, Colombia. This research was realized in the Experimental Center of the National University in Palmira. A design of a Randomized Completed Blocks, with six treatments and three repeticions was used. The treatments were two accesions of guandul (tree and shrub Cajanus cajan, Canavalia Canavalia ensiformis Corn Zea mays, Star grass Cynodon niemfuensis and Ramkin Cucúrbita moschata. The plot size was 20 m x 10 m. The materials were incorporated with tractor and disk plough when the maiz was colected as ear. The results showed that corn and Canavalia had the higher yields of fitomass (10068 and 9748 kg/ha, respectively. The legumes C. cajan (tree and C. ensiformis showed higher yileds of Nitrogen (254 and 213 kg/ha and biomolecules. Z. mays and C. nlemfuensis were the treatments with lower

  18. Evaluación de seis abonos verdes en un vertisol ústico en condiciones del valle del cauca

    OpenAIRE

    Bonilla C. C. R.; Sánchez M. S.; Salamanca W. F.

    2006-01-01

    En el Centro Experimental de la Universidad Nacional de Colombia-Sede Palmira, en un diseño de bloques completos al azar, se evaluaron seis tratamientos con tres repeticiones. (Cajanus cajan guandul arbóreo y arbustivo; Canavalia ensiformis, fríjol Canavalia; Cynodon niemfuensis, pasto estrella; Zea mays, Maíz y Cucúrbita moschata, zapallo). El pasto estrella y el zapallo fueron cultivos previos al ensayo; los otros materiales se sembraron por semilla a 50 cm en cuadro y se incorporaron al su...

  19. Tolerancia de cinco leguminosas al aluminio en solución nutritiva.

    OpenAIRE

    Pérez N Juan Carlos; Osorio V Nelson Walter; Lotero C. Jaime

    2011-01-01

    Se realizó una investigación para determinar el grado de tolerancia a aluminio (Al) de cinco leguminosas tropicales: guandul (Cajanus cajan L. Mills), canavalia (Canavalia ensiformis L.), frijol jacinto (Dolichos lablab L.), leucaena (Leucaena leucocephala (C. Lam.) De Witt) y fríjol terciopelo (Stizolobium deeringianum Bort.). Las plantas crecieron bajo cinco concentraciones de Al: 0, 2, 4, 8 y 16 mg L-1 en solución nutritiva. La leguminosa más afectada por las concentraciones de Al fue L. l...

  20. Attraction of Neomegalotomus parvus (Westwood)(Heteroptera: alydidae) to cow urine and ammonia Atração de Neomegalotomus parvus (Westwood) (Heteroptera: alydidae) por urina de vaca e amônia

    OpenAIRE

    Jovenil José da Silva; Iara Cintra de Arruda-Gatti; Adriana Yatie Mikami; Aline Pissinati; Antônio Ricardo Panizzi; Mauricio Ursi Ventura

    2010-01-01

    The alydid bug parvus (Westwood) is not easily detected in the field and the discovery of attractants may be a suitable way to monitor this species. The attraction of N. parvus to traps (transparent, transparent green and yellow) baited with cow urine and ammonia was studied in two field trials. Traps were placed near a terrace with pigeon pea plants (Cajanus cajan L.). Tap water, NaCl 10% aqueous solution (w/v), cow urine, and ammonia (NH4OH 1% aqueous solution) were tested as attractants in...

  1. OS SISTEMAS AGROFLORESTAIS COMO ALTERNATIVA ECONÔMICA EM PEQUENAS PROPRIEDADES RURAIS: ESTUDO DE CASO

    OpenAIRE

    Mário Jorge Campos dos Santos; Samantha Nazaré de Paiva

    2002-01-01

    Os Sistemas Agroflorestais (SAFs) constituem-se em uma alternativa de uso da terra para aliar a estabilidade do ecossistema visando à eficiência e otimização de recursos naturais na produção de forma integrada e sustentada. O objetivo deste estudo de caso foi avaliar a viabilidade econômica de um sistema agroflorestal localizado na região do Pontal do Paranapanema. O SAF em estudo é formada por espécies agrícolas: milho (Zea maiz), feijão guandu (Cajanus cajan), carioquinha, (Vigna unguiculat...

  2. Plant residues: short term effect on sulphate, borate, zinc and copper adsorption by an acid oxisol

    Directory of Open Access Journals (Sweden)

    Dias Ana Cristi Basile

    2003-01-01

    Full Text Available Laboratory experiments were carried out to examine the effects of plant residues on Cu, Zn, B and S adsorption by an acidic oxisol. The plant residues were: black oats (Avena strigosa, oil seed radish(Raphanus sativus, velvet beans (Stizolobium cinereum, and pigeon pea (Cajanus cajan collected at flowering stage. Plant residues increased Cu and Zn adsorptions and decreased B and S adsorptions. The results indicated that for short term effect plant residues decreased the availabilities of Cu and Zn through metal organic complex reactions and increased availabilities of S and B through competition with organic anions by the adsorption sites on soil.

  3. Efeito de leguminosas nas características químicas e matéria orgânica de um solo degradado Effect of leguminous crops on the chemical characteristics and organic matter of a degraded soil

    Directory of Open Access Journals (Sweden)

    João T. Nascimento

    2003-12-01

    Full Text Available Com a finalidade de avaliar o efeito de leguminosas nas características químicas de um Luvissolo degradado instalou-se, no município de Alagoinha, PB, um experimento, de 1997-99, com doze espécies: crotalária (Crotalaria juncea, L, guandu (Cajanus cajan,L, guandu-anão (Cajanus cajan (L Millsp, calopogônio (Calopogonium mucunoides, L, feijão-de-porco (Canavalia ensiforme, L, lab-lab (Dolichos lab lab, L, kudzu tropical (Pueraria phaseoloides, L, siratro (Macroptilium atropurpureum, L, leucena (Leucaena leucocephala, L, cunhã (Clitoria ternatea, L, mucuna preta (Styzolobium aterrimum, L e mucuna cinza (Styzolobium cinereum Piper e Tracy além do tratamento testemunha. Nesse estudo, o solo do experimento não recebeu adubo mineral nem corretivo. Avaliaram-se as características químicas do solo, nas profundidades 0-10, 10-20 e 20-30 cm. Os resultados mostraram efeitos significativos das leguminosas sobre a fertilidade do solo, em comparação com a testemunha, com incrementos significativos de pH e de cátions trocáveis, refletindo positivamente na CTC e no índice de saturação por bases. Na matéria orgânica não ocorreu efeito significativo entre leguminosas e testemunha.With the purpose of evaluating the effect of leguminous crops on the chemical characteristics of a degraded Alfisol, in the municipal district of Alagoinha, PB, Brazil, an experiment was conducted during 1997-99 with twelve species: Crotalaria juncea L, Cajanus cajan L, Cajanus cajan (L Millsp, Calopogonium mucunoides L, Canavalia ensiformis L, Dolichos lab lab L, Pueraria phaseoloides L, Macroptilium atropurpureum L, Leucaena leucocephala L, Clitoria ternatea L, Styzolobium aterrimum L, and Styzolobium cinereum Piper and Tracy besides a control treatment. In this study, the soil of the experimental site did not receive mineral fertilizer nor any corrective. The chemical characteristics of the soil were evaluated at 0-10, 10-20 and 20-30 cm depths. The results

  4. Cytoplasmic male sterility-associated chimeric open reading frames identified by mitochondrial genome sequencing of four Cajanus genotypes.

    Science.gov (United States)

    Tuteja, Reetu; Saxena, Rachit K; Davila, Jaime; Shah, Trushar; Chen, Wenbin; Xiao, Yong-Li; Fan, Guangyi; Saxena, K B; Alverson, Andrew J; Spillane, Charles; Town, Christopher; Varshney, Rajeev K

    2013-10-01

    The hybrid pigeonpea (Cajanus cajan) breeding technology based on cytoplasmic male sterility (CMS) is currently unique among legumes and displays major potential for yield increase. CMS is defined as a condition in which a plant is unable to produce functional pollen grains. The novel chimeric open reading frames (ORFs) produced as a results of mitochondrial genome rearrangements are considered to be the main cause of CMS. To identify these CMS-related ORFs in pigeonpea, we sequenced the mitochondrial genomes of three C. cajan lines (the male-sterile line ICPA 2039, the maintainer line ICPB 2039, and the hybrid line ICPH 2433) and of the wild relative (Cajanus cajanifolius ICPW 29). A single, circular-mapping molecule of length 545.7 kb was assembled and annotated for the ICPA 2039 line. Sequence annotation predicted 51 genes, including 34 protein-coding and 17 RNA genes. Comparison of the mitochondrial genomes from different Cajanus genotypes identified 31 ORFs, which differ between lines within which CMS is present or absent. Among these chimeric ORFs, 13 were identified by comparison of the related male-sterile and maintainer lines. These ORFs display features that are known to trigger CMS in other plant species and to represent the most promising candidates for CMS-related mitochondrial rearrangements in pigeonpea. PMID:23792890

  5. Comportamento de espécies de adubos verdes em diferentes épocas de semeadura e espaçamentos na região dos Cerrados

    Directory of Open Access Journals (Sweden)

    Amabile Renato Fernando

    2000-01-01

    Full Text Available Com o objetivo de avaliar o crescimento e desenvolvimento de leguminosas utilizadas como adubos verdes, instalaram-se três ensaios, em três épocas de semeadura e dois espaçamentos na região dos Cerrados, durante o ano agrícola de 1991/1992, na área experimental da Embrapa-Centro Nacional de Pesquisa do Solo (CNPS, em Senador Canedo, GO. As espécies avaliadas foram Crotalaria juncea L., mucuna-preta (Mucuna aterrima (Piper & Tracy Merr., guandu cv. Kaki (Cajanus cajan (L. Millsp. e Crotalaria ochroleuca G. Don. O delineamento experimental utilizado, dentro de cada época, foi o de blocos ao acaso com parcelas subdivididas, com três repetições. Os resultados indicaram que C. juncea e C. cajan apresentaram as maiores produções de fitomassa seca. O atraso da semeadura, em relação ao início da estação chuvosa, reduziu os rendimentos de fitomassas verde e seca produzidos pelas leguminosas, exceto pela mucuna-preta. Os espaçamentos de 0,5 m e 0,4 m não influenciaram o período para o florescimento e as produções de fitomassas verde e seca.

  6. Evaluation of N2-Fixation Capacity of Leguminous Shrub Plants

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    N2-fixation percentages in the aerial parts of the first and second cuttings of five species of leguminous shrub plants, i.e., Lespedeza formosa (Vog.) Koehne from Jiangxi, Lespedeza pilosa (Thunb.) Sieb et Zucc., Lespedeza formosa (Vog.) Koehne from Zhejiang, Cajanus cajan (L.) Millsp. and Indigofera pseudotinctoria Mats., of three genera were investigated in terms of pot culture experiment using natural 15N abundance method with Bischofia javanica Bl., Camptotheca acuminata Decne. and Gardenia jasminoides Ellis as reference plants. Results showed that the N2-fixation percentages were different for the three genera of leguminous shrubs but almost the same for three species in the same genus. I. pseudotinctoria Mats. had the highest N2-fixation percentage in its aerial part, and then followed by Lespedeza and C. cajan. The N2-fixation percentages in the aerial parts of the plants that had grown for three years tended to be lower than those of the one-year-old plants except L. formosa (Vog.) Koehne from Zhejiang and L. formosa (Vog.) Koehne from Jiangxi.

  7. Production et coûts des semences utilisées pour l'amélioration des jachères et des aménagements antiérosifs : "Le cas des zones de savane du Nord Cameroun"

    Directory of Open Access Journals (Sweden)

    Dugué, P.

    1999-01-01

    Full Text Available Seeds Production for Fallow and Anti-Erosion Management : "The Case of the Savannah Areas in North Cameroon". The maintenance of cultivated soils' fertility in North Cameroon relies on the extension of the techniques of water erosion control and improved legume crop fallows. These innovations require that seeds must become available to farmers. These seeds are legume seeds (Calopogonium mucunoides, Stylosanthes hamata, Mucuna pruriens, Cajans cajan or perennial grasses (Panicum maximum, Andropogon gayanus, Cenchrus ciliaris. On station research has developed the techniques of seeds production which can be introduced in the farmer's environment on the one hand, and on the other hand can be used to evaluate the cost of legumes seeds production. Depending on the species, the total price of seeds production varies from 250 to 1160 FCFA/kg (0, 5 U$ to 2 U$/kg. Based on these prices, it seems possible in the first step to diffuse these seeds varieties. The diffusion of the vegetal material depends firstly on the interest that farmers attach to techniques of soil restauration which are proposed to them. If they adopt these innovations, they can easily produce seeds that they need after been trained to the techniques developed by research.

  8. Cassia renigera Wall.: novo hospedeiro de Ceratocystis fimbriata Ell. & Halst. Cassia renigera Wall.: a new host of Ceratocystis fimbriata Ell. & Halst.

    Directory of Open Access Journals (Sweden)

    Ivan José Antunes Ribeiro

    1987-01-01

    Full Text Available Ceratocystis fimbriata foi descrito pela primeira vez em 1984, causando murcha em plantas de Cassia renigera, em Campinas (SP. Foram realizadas inoculações cruzadas com dois isolados de C. fimbriata obtidos de Cassia renigera e mangueira (Mangifera indica em plantas de: acácia-negra (Acacia decurrens, cacaueiro (Theobroma cacao, crotalária (Crotalaria juncea, feijão-guandu (Cajanus cajan, figueira (Ficus carica, gamelina (Gmelina arborea, mangueira (Mangifera indica, seringueira (Hevea brasilienses, Cassia sp., C. carnaval, C. ferruginea, C. grandis, C. moschata, C. multijuga, C. nodosa, C. renigera, C. siamea e C. speciosa. Os dois isolados do fungo foram patogênicos a todas as plantas testadas, com exceção de cacaueiro, gamelina e C. grandis.Ceratocystis fimbriata Ell. & Halst. is described for the first time in 1984 on Cassia renigera, in Campinas, State of São Paulo, Brazil. In cross inoculation, the isolates of C. fimbriata obtained from Cassia renigera and from Mangifera indica showed pathogenicity to the following plants: Acacia decurrens, Theobroma cacao, Crotalaria juncea, Cajanus cajan, Ficus Carica, Gmelina arborea, Mangifera indica, Hevea brasiliensis, Cassia sp., C. carnaval, C. ferruginea, C. grandis, C. moschata, C. multijuga, C. nodosa, C. renigera, C. siamea and C. speciosa. The isolates of C. fimbriata were pathogenic to all tested plants, except for Theobroma cacao, Gmelina arborea and C. grandis.

  9. Descrição botânica de linhagens puras selecionadas de guandu Botanical description of selected pigeon-pea pure lines

    Directory of Open Access Journals (Sweden)

    Milena Provazi

    2007-04-01

    Full Text Available Este estudo foi desenvolvido com o objetivo de realizar a descrição botânica de 11 linhagens de Cajanus cajan. Os principais descritores foram: hábito de crescimento, altura de plantas (cm, número de ramos, diâmetro do caule (mm, cor básica da flor, cor básica da vagem, cor das sementes, cor em volta do hilo das sementes, largura do hilo e forma das sementes. Os descritores utilizados mostraram-se suficientes para caracterizar as linhagens com características distintas, pelas quais podem ser facilmente identificadas.This work aimed to perform the morphologic characterization of eleven previously selected Cajanus cajan lines. The main descriptors were: plant height (cm, number of branches, stem diameter (mm, basic flower color, basic pod color, seed color, seed hilum color, hilum thickness, and seed shape. The descriptors used were sufficient to characterize the lines with distinct characteristics. Using these descriptors the lines may be easily identified.

  10. Cytoplasmic male sterility-associated chimeric open reading frames identified by mitochondrial genome sequencing of four Cajanus genotypes.

    Science.gov (United States)

    Tuteja, Reetu; Saxena, Rachit K; Davila, Jaime; Shah, Trushar; Chen, Wenbin; Xiao, Yong-Li; Fan, Guangyi; Saxena, K B; Alverson, Andrew J; Spillane, Charles; Town, Christopher; Varshney, Rajeev K

    2013-10-01

    The hybrid pigeonpea (Cajanus cajan) breeding technology based on cytoplasmic male sterility (CMS) is currently unique among legumes and displays major potential for yield increase. CMS is defined as a condition in which a plant is unable to produce functional pollen grains. The novel chimeric open reading frames (ORFs) produced as a results of mitochondrial genome rearrangements are considered to be the main cause of CMS. To identify these CMS-related ORFs in pigeonpea, we sequenced the mitochondrial genomes of three C. cajan lines (the male-sterile line ICPA 2039, the maintainer line ICPB 2039, and the hybrid line ICPH 2433) and of the wild relative (Cajanus cajanifolius ICPW 29). A single, circular-mapping molecule of length 545.7 kb was assembled and annotated for the ICPA 2039 line. Sequence annotation predicted 51 genes, including 34 protein-coding and 17 RNA genes. Comparison of the mitochondrial genomes from different Cajanus genotypes identified 31 ORFs, which differ between lines within which CMS is present or absent. Among these chimeric ORFs, 13 were identified by comparison of the related male-sterile and maintainer lines. These ORFs display features that are known to trigger CMS in other plant species and to represent the most promising candidates for CMS-related mitochondrial rearrangements in pigeonpea.

  11. Efeitos de quantidades de fitomassa de adubos verdes na supressão de plantas daninhas Effects of cover crop biomass quantities on weed suppression

    Directory of Open Access Journals (Sweden)

    F.J. Severino

    2001-08-01

    Full Text Available Com o objetivo de avaliar as correlações entre a utilização de diferentes quantidades de fitomassa, incorporada ou na superfície do solo, dos adubos verdes amendoim-forrageiro (Arachis pintoi, crotalária (Crotalaria juncea e feijão-guandu-anão (Cajanus cajan na redução da emergência e produção de fitomassa seca das plantas daninhas capim-braquiária (Brachiaria decumbens, capim-colonião (Panicum maximum e picão-preto (Bidens pilosa, foi instalado um experimento em casa de vegetação do Departamento de Produção Vegetal da ESALQ/USP-Piracicaba. O delineamento experimental utilizado foi o de blocos casualizados, com os tratamentos em quatro repetições, em esquema fatorial 3 x 6 x 3 x 2, sendo três adubos verdes (A. pintoi, C. juncea e C. cajan, seis quantidades de fitomassa (0; 0,5; 1; 2; 4; e 8 vezes a quantidade de adubo verde produzido pela média de campo, três plantas daninhas (B. decumbens, P. maximum e B. pilosa e duas formas de distribuição da fitomassa dos adubos verdes (na superfície do solo e de 0 a 5 cm de profundidade. As características avaliadas durante a condução do experimento foram as seguintes: densidade de plantas daninhas/vaso e produção de fitomassa seca das plantas daninhas (g/vaso, determinadas aos 15 e aos 30 dias após a instalação do experimento. Os resultados permitiram concluir que a fitomassa dos adubos verdes, nas duas formas utilizadas, reduz significativamente as populações das plantas daninhas testadas. As correlações ocorrem de maneira distinta pelos diferentes adubos verdes, sendo o feijão-guandu-anão, de forma geral, o mais efetivo na supressão das plantas daninhas.Different biomass quantities of the cover crops Arachis pintoi Krapov. & W.C. Gregory, Crotalaria juncea and Cajanus cajan were either incorporated or spread on the surface of the soil to study their effect on the emergence and dry mass production of the weeds Brachiaria decumbens, Panicum maximum and Bidens

  12. 干热河谷典型区土壤功能对不同植被恢复措施的响应%Response of Soil Functioning to Ecological Restoration Practices in the Typical Arid-Hot Valley

    Institute of Scientific and Technical Information of China (English)

    纪中华; 李建增; 闫帮国; 钱坤建; 潘志贤; 奎建蕊

    2012-01-01

    To explore the response of soil function in arid-hot ecosystem to restoration treatments,the soil infiltration,physical and chemical properties under different restoration models were measured.The results showed that mixture plantation-Cajanus cajan+Pennisetum purpureum had the most effective function on soil in Tamarindus indica forest.The proportion of soil aggregation increased up to 60%~80%,organic matter of soil surface increased by a factor up to 5.01 times,infiltration rate increased by a factor up to 14.43 times compared to the control(T.indica only).The maximum water holding storage reached up to 633.0 t/hm2.All of the soil characters measured in the C.cajan+P.purpureum model were obviously higher than other models and this also enhanced T.indica productivity.The soil functions of other models were poorer than C.cajan+P.purpureum,but better than the control.The results indicated that the improving effects of soil function were determined by plant species and their combinations.%为研究干热河谷地区不同恢复措施下土壤功能的变化,分析了不同模式下土壤入渗和理化特征。结果表明,在乡土树种罗望子(Tamarindus indica)林下,木豆(Cajanus cajan)+象草(Pennisetum purpureum)模式的恢复效果最好,土壤团粒达60%~80%,表层有机质含量较对照提高了5.01倍,其入渗率较对照区提高14.43倍,最大吸持贮水量可达633.0t/hm2,明显高于其他模式,土壤性质的改善也提高了罗望子的生产力水平。其他恢复模式的土壤功能较差,但优于对照模式,表明土壤理化性质的改良取决于植物种类的选择和配置。

  13. Fitomassa de adubos verdes e cobertura do solo na região do Alto Vale do Jequitinhonha, Minas Gerais = Biomass of green manure and soil cover in the region of Vale do Jequitinhonha, Minas Gerais-Brazil.

    Directory of Open Access Journals (Sweden)

    Evander Alves Ferreira

    2012-08-01

    Full Text Available A adubação verde constitui prática cultural de relevante impacto positivo em sistemas de produção agrícola que visam o uso sustentável da terra. Objetivou-se com esse trabalho avaliar o desempenho de seis leguminosas utilizadas para adubação verde, quanto à produtividade de fitomassa, cobertura do solo e uso da radiação fotossinteticamente ativa na região conhecida como Alto Vale do Jequitinhonha em Minas Gerais. O experimento foi conduzido entre os meses de março a outubro de 2010, em delineamento em blocos casualizados com quatro repetições. Os adubos verdes utilizados foram: crotalária (Crotalariaspectabilis, feijão-de-porco (Canavalia ensiformis, feijão-guandu-anão (Cajanus cajan, lab-lab (Lablab purpureus, mucuna-anã (Mucuna deenringiana e tremoço-branco (Lupinus albus L.. Os adubos verdes apresentaram elevadas produções de fitomassa, mas com diferentes picos de cobertura do solo e interceptação da radiação. Os maiores acúmulos de matéria seca e fresca foram observados pela crotalária seguido pelo feijão-guandu-anão. Entre as espécies estudadas, o feijão-de-porco é aque apresentou maior período vegetativo, representando elevado potencial para recobrimento do solo, destacando-se também na interceptação de radiação fotossinteticamente ativa.Green manuring is a positive practice that reduces the impact on agricultural production systems aimed at sustainable land use. The objective of this study was to evaluate the performance of six legumes used for green manure, the productivity of biomass, land cover and use of photosynthetic active radiation in the region of the Vale do Jequitinhonha, Minas Gerais, Brazil. The experiment was conducted during March to October, 2010 in randomized block design with four replications. Green manures were used: Crotalaria spectabilis, Canavalia ensiformis, Cajanus cajan, Lablab purpureus, Mucuna deenringiana and Lupinus albus. Green manures had high yields of biomass

  14. Selection of species tolerant to the herbicide sulfentrazone with potential for phytoremediation of contaminated soilsSeleção de espécies tolerantes ao herbicida sulfentrazone com potencial para a fitorremediação de solos contaminados

    Directory of Open Access Journals (Sweden)

    João Carlos Madalão

    Full Text Available This paper aimed to select species with potential for phytoremediation of soils contaminated with the herbicide sulfentrazone. Eight species were evaluated: Arachis pintoi, Eleusine coracana, Crotalaria spectabilis, Crotalaria ochroleuca, Cajanus cajan, Leucaena leucocephala, Stizolobium cinereum, and Raphanus sativus. The experiment was set-up inside a greenhouse, using pots with a capacity of 6dm3 filled with soil samples collected at a depth of 0-20cm. The experimental design was arranged into randomised blocks in a factorial scheme (8 × 5 with four replications, which consisted of the combination between the species and five doses of sulfentrazone (0, 200, 400, 800, and 1,600g ha-1. The herbicide phytotoxicity, plant heights, and dry masses of shoots and roots were evaluated. The species Cajanus cajan and Leucaena leucocephala had a higher tolerance to sulfentrazone up to a dose of 400g ha-1, showing minor symptoms of phytotoxicity and smaller decreases in plant heights and in dry matter accumulation, both in the shoots and roots, when compared to the control treatment, indicating, thus, the potential to be used for further studies on phytoremediation of sulfentrazone in soil. Este trabalho teve como objetivo selecionar espécies com potencial para a fitorremediação de solos contaminados com o herbicida sulfentrazone. Foram avaliadas oito espécies: Arachis pintoi, Eleusine coracana, Crotalaria spectabilis, Crotalaria ochroleuca, Cajanus cajan, Leucaena leucocephala, Stizolobium cinereum e Raphanus sativus. O experimento foi instalado em casa de vegetação, com a utilização de vasos com capacidade para 6 dm3 preenchidos com porções de solo coletadas na profundidade de 0-20 cm. O delineamento experimental foi o de blocos casualizados em esquema fatorial 8 × 5, com quatro repetições, composto pela combinação entre as espécies e cinco doses do sulfentrazone (0, 200, 400, 800 e 1.600 g ha-1. Foram avaliadas a fitotoxicidade do

  15. Effect of dose of gamma-rays and ethylmethane sulphonate on the germination and survival of induced mutations in pigeonpea

    Energy Technology Data Exchange (ETDEWEB)

    Premsekar, S. (Central Inst. for Cotton Research, Coimbatore (India). Regional Station); Appadurai, R. (Tamil Nadu Agricultural Univ., Coimbatore (India))

    1981-06-01

    The LD/sub 50/ values for germination and survival of the induced mutants (M/sub 1/) of pigeonpea (Cajanus cajan (Linn.) Millsp.) were attained with 20 and 15 krad when gamma-rays were used, and 30 and 40 mM concentration when EMS was used. In the combination treatments the half-kill dose for germination and survival was reached even at the low dose combination of 5 krad gamma-ray + 20 mM EMS. Higher doses resulted in lower pollen and seed fertility. The sterility was much enhanced in the combined treatments. The number of pods, seed yield and weight of seeds could be stimulated with 10 and 15 krad doses of gamma-rays and 20 mM of EMS. In combination treatments such a stimulatory effect was noticed in seed weight only.

  16. Impact of mine waste dumps on growth and biomass of economically important crops.

    Science.gov (United States)

    Mathiyazhagan, Narayanan; Natarajan, Devarajan

    2012-11-01

    The present study aimed to investigate the effect of magnesite and bauxite waste dumps on growth and biochemical parameters of some edible and economically important plants such as Vigna radiata, V. mungo, V. unguiculata, Eleusine coracana, Cajanus cajan, Pennisetum glaucum, Macrotyloma uniflorum, Oryza sativa, Sorghum bicolour, Sesamum indicum, Ricinus communis, Brassica juncea, Gossypium hirsutum and Jatropha curcas. The growth rate of all the crops was observed in the range of 75 to 100% in magnesite and 15 to 100% in bauxite mine soil. The moisture content of roots and shoots of all the crops were in the range of 24 to 77, 20 to 88% and 42 to 87, 59 to 88% respectively. The height of the crops was in the range of 2.6 to 48 cm in magnesite soil and 3 to 33 cm in bauxite soil. Thus the study shows that both mine soils reflects some physical and biomolecule impact on selected crops. PMID:23741803

  17. Response of Seed Germination of Suitable Shrubs to Drought Stress in Karst Area%干旱胁迫对喀斯特地区适生灌木种子萌发的影响

    Institute of Scientific and Technical Information of China (English)

    陈莹; 李龙兴; 赵丽丽

    2015-01-01

    为岩溶石漠化地区的生态恢复与重建提供依据,以不同浓度 PEG-6000溶液(0%,5%,10%,20%)模拟干旱胁迫,研究胡枝子(Lespedeza bicolor Turcz)、截叶铁扫帚(Lespedeza cuneata G.Don)、白刺花(Sophora davidii Skeels)和木豆(Cajanus cajan Millsp)种子发芽率及萌发期的生理生化指标对干旱胁迫的响应,探讨喀斯特地区灌木种子萌发对干旱环境的适应机制。结果表明:干旱胁迫下4种灌木种子发芽率均显著降低(p<0.05)。随着 PEG-6000浓度的增加,4种灌木种子萌发期的 SOD 活性呈现不同的变化趋势,胡枝子和截叶铁扫帚种子萌发期 SOD 活性呈先上升后降低的趋势(p <0.05),白刺花和木豆种子SOD 活性逐渐上升;4种灌木种子萌发期的 POD 活性变化趋势相同,均呈下降趋势,CAT 活性与 GSH 含量呈先上升后降低的趋势;胡枝子、铁扫帚与木豆种子萌发期 SP 含量呈先显著上升(p <0.05)后降低的趋势,白刺花却逐渐显著上升(p<0.05)。白刺花对干旱胁迫适应能力最强,其次是铁扫帚和胡枝子。%To provide a reference for ecological restoration and reestablishment in karst regions,the seed germination, physiological and biochemical indexes of Lespedeza bicolor , Lespedeza cuneata , Sophora davidii and Cajanus cajan were investigated under different concentrations of PEG-6000 solution (0,5%,10% and 20%)in germinating period.Adaptive mechanism of shrub seed germination in arid environment was discussed either.Results: Seed germination of four species of shrubs significantly decreased under drought stress(p <0.05).With increasing PEG concentration,the SOD activity showed different trends between different materials,the L.bicolor and L.cuneata generally first increased and then decreased(p<0.05),but the SOD activity of S.davidii and C.cajan increased gradually;POD activity of four species of shrubs

  18. Apoptosis in liver cancer (HepG2) cells induced by functionalized gold nanoparticles.

    Science.gov (United States)

    Ashokkumar, Thirunavukkarasu; Prabhu, Durai; Geetha, Ravi; Govindaraju, Kasivelu; Manikandan, Ramar; Arulvasu, Chinnasamy; Singaravelu, Ganesan

    2014-11-01

    An ethnopharmacological approach for biosynthesis of gold nanoparticles is being demonstrated using seed coat of Cajanus cajan. Medicinal value of capping molecule investigated for anticancer activity and results disclose its greater potential. The active principle of the seed coat [3-butoxy-2-hydroxypropyl 2-(2,4-dihydroxyphenyl) acetate] is elucidated. Rapid one-step synthesis yields highly stable, monodisperse (spherical) gold nanoparticles in the size ranging from 9 to 41 nm. Anticancer activity has been studied using liver cancer cells and cytotoxic mechanism has been evaluated using MTT, Annexin-V/PI Double-Staining Assay, Cell cycle, Comet assay and Flow cytometric analysis for apoptosis. The present investigation will open up a new possibility of functionalizing gold nanoparticles for apoptosis studies in liver cancer cells. PMID:25444656

  19. Sistema em fluxo para determinação espectrofotométrica de uréia em plasma de sangue animal empregando leguminosa como fonte natural da enzima urease

    Directory of Open Access Journals (Sweden)

    Luca Gilmara C.

    2001-01-01

    Full Text Available A spectrophotometric flow injection analysis (FIA procedure employing natural urease enzyme source for the determination of urea in animal blood plasma was developed. Among leguminous plants used in the Brazilian agriculture, the Cajanus cajan specie was selected as urease source considering its efficiency and availability. A minicolumn was filled with leguminous fragments and coupled to the FIA manifold, where urea was on-line converted to ammonium ions and subsequently it was quantified by spectrophotometry. The system was employed to determine urea in animal plasma samples without any prior treatment. Accuracy was assessed by comparison results with those obtained employing the official procedure and no significant difference at 90 % confidence level was observed. Other profitable features such as an analytical throughput of 30 determinations per hour, a reagent consumption of 19.2 mg sodium salicylate, 0.5 mg sodium hipochloride and a relative standard deviation of 1.4 % (n= 12 were also obtained.

  20. Identification of Meloidogyne enterolobii in Guangdong Province and Hainan Province%广东省和海南省象耳豆根结线虫的鉴定

    Institute of Scientific and Technical Information of China (English)

    卓侃; 胡茂秀; 廖金铃; 崔汝强; 李迅东; 王彬; 周健勇

    2008-01-01

    运用线虫比较形态学、结合同工酶、mtDNA-PCR、rDNA-IGS-PCR技术,对采自中国广东和海南2个省的根结线虫进行鉴定.在海南省定安的木豆(Cajanus cajan)、海南省海口的木豆和番石榴(Psidium guaja-va)、广东省番禺的辣椒(Capsicum annuum)和南瓜(Cucrbita mosechata)及广东省遂溪的豇豆(Vigna sinensis)上发现象有耳豆根结线虫(Meloidogyne enterolobii).这是首次在海南省外发现象耳豆根结线虫,同时还发现该线虫的2个新寄主,即木豆和南瓜.

  1. Isolation and Identification of Vitexin and Isovitexin in Herba Cajani%木豆中牡荆甙与异牡荆甙的分离与鉴定

    Institute of Scientific and Technical Information of China (English)

    林励; 谢宁; 程紫骅; 魏敏; 刘秀芬; 刘艳平

    1999-01-01

    为进一步了解木豆(Cajanus cajan L.Millsp) 嫩茎叶的化学成分,以溶剂法、结晶法及柱层析法从木豆中将牡荆甙(vitexin)与其异构体异牡荆甙(isovitexin)分离,并以红外光谱法(IR)、紫外光谱法(UV)、质谱法(Ms)、13C 谱法 (13CNMR)、1H谱法(1HNMR ) 确定了两者的结构,其中异牡荆甙系首次从木豆中分得.

  2. 木豆:一种多用途的优良作物

    Institute of Scientific and Technical Information of China (English)

    杨示英

    2001-01-01

    木豆(学名:Cajanus cajan(L.)Millsp.英文名:pigeonpea或Red Gram),原产于非洲和亚洲的热带,又名鸽豆、树黄豆、千年豆等,是豆科。木豆属一年生或多年生木本植物,具有粮食、蔬菜、饲料、柴火、放养紫胶虫等功能,还可作覆盖作物、蜜源作物、防护林、薪碳林、药用植物等,用途很广。木豆是迄今为止唯一的一种木本食用豆类作物,全身都是宝,

  3. 南友高速公路边坡5种地被植物的适应性研究

    Institute of Scientific and Technical Information of China (English)

    和太平; 李玉梅; 陆山风; 唐忠国; 文祥凤; 陈燕; 唐虹; 屈婷婷

    2011-01-01

    在调查广西南(宁)一友(谊关)高速公路绿化植物现状的基础上,对高速公路边坡种植面积最大、出现频率最高的山毛豆(Tephrosia candida)、木豆(Cajanus cajan)、猪屎豆(Crotalaria pallida)、南美蟛蜞菊(Wedelia trilobata)、糖蜜草(Melinis minutiflora)等5种主要地被植物的生物学、生态习性、生态功能、观赏功能等基本特征进行总结,分析与评价其在该高速公路边坡的适应性,探讨其栽培养护管理技术问题,并提出建议.

  4. 文山州印度木豆引种示范及配套技术试验研究取得新进展

    Institute of Scientific and Technical Information of China (English)

    张廷宏

    2004-01-01

    木豆,学名Cajanus Cajan (L.) Mjllspaugh是豆科蝶形花亚科,木豆属(Cajanus)中的栽培种。木豆又称树豆、鸽豆、三叶豆、黄豆树、柳豆等。它起源于南亚的印度半岛,印度是木豆的起源和变异中心,故人们常把从印度引进的木豆叫印度木豆。木豆广泛分布于亚洲、非洲和拉丁美洲,亚洲的主要生产国有印度(种植面积360万hm2)、

  5. Estratégias de utilização de adubos verdes no re-desenho da paisagem agrícola e melhoria da fertilidade do solo

    OpenAIRE

    Castro, Cristina Maria de; Devide, Antonio Carlos Pries

    2015-01-01

    Foi avaliado, por dois anos, a utilização de adubos verdes na formação de palhada para plantio direto, e em aléias separando as áreas de cultivo de culturas alimentícias, e sua ação na melhoria da fertilidade do solo. Faixas foram semeadas com dois pré-cultivos: sorgo (Sorghum bicolor) e coquetel [sorgo (Sorghum bicolor cv.BRS 610) + Crotalaria juncea]; separadas por fileiras duplas de Cajanus cajan cv. Mandarim; as sub-parcelas receberam resíduo da poda do guandu por ocasião da roçada dos ad...

  6. Tolerancia de cinco leguminosas al aluminio en solución nutritiva.

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    Pérez N Juan Carlos

    2003-06-01

    Full Text Available Se realizó una investigación para determinar el grado de tolerancia a aluminio (Al de cinco leguminosas tropicales: guandul (Cajanus cajan L. Mills, canavalia (Canavalia ensiformis L., frijol jacinto (Dolichos lablab L., leucaena (Leucaena leucocephala (C. Lam. De Witt y fríjol terciopelo (Stizolobium deeringianum Bort.. Las plantas crecieron bajo cinco concentraciones de Al: 0, 2, 4, 8 y 16 mg L-1 en solución nutritiva. La leguminosa más afectada por las concentraciones de Al fue L. leucocephala, la cual exhibió una disminución significativa en la producción de materia seca y en la traslocación de P hacia la parte aérea. En las otras leguminosas la materia seca y la distribución de P fueron poco afectados.

  7. Differential responses of photosynthetic parameters of pigeonpea and amaranth leaf discs to SO{sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Saraswathi, J.; Rao, K.V.M. [Andhra University, Visakhapatnam (India). Dept. of Botany

    1995-08-01

    The reduction in chlorophyll (Chl) and protein contents and the increase in amino acid content in leaf discs in response to aqueous SO{sub 2} exposure under continuous irradiance were more expressed in Amaranthus paniculatus (C-4 plant) than in Cajantus cajan (C-3 plant). The content of SH-compounds increased more in pigenonpea than in amaranth leaf discs in response to SO{sub 2}. Aqueous SO{sub 2} exposure also reduced the CO{sub 2} fixation and ribulose-1,5-bisphosphate carboxylase (RuBPC) and phosphoenolpyruvate carboxylase (PEPC) activities in leaf discs of both plant species. The differences in sensitivity of these plants to SO{sub 2} were related to their conversion efficiency of SO{sub 2} to less toxic substances and sulphydryl compounds.

  8. Hydroalcoholic extracts of Indian medicinal plants can help in amelioration from oxidative stress through antioxidant properties.

    Science.gov (United States)

    Sarkar, Rhitajit; Mandal, Nripendranath

    2012-01-01

    The in vitro study of the antioxidant properties of the hydroalcoholic extracts of various Indian medicinal plants can logically help to develop a better and safer way of amelioration from oxidative stress. As aimed, the present study has been done to estimate and thereby conclude regarding the antioxidant activities of a few Indian medicinal plants, viz., Terminalia chebula, Terminalia belerica, Emblica officinalis, Caesalpinia crista, Cajanus cajan, and Tinospora cordifolia. The extracts of the plants have been subjected to the evaluation of antioxidant properties through scavenging assays for reactive oxygen species like superoxide, nitric oxide, peroxynitrite, hypochlorous acid, singlet oxygen, etc. and measurement of TEAC values and other phytochemical parameters. The phenolic and flavonoid contents of each plant have been found to be correlated to their individual antioxidant activity. The results showed the hydroalcoholic extracts of the plants were efficient indicators of their antioxidant capacity thus concreting their basis to be used as natural antioxidant. PMID:22624183

  9. Chemical Characteristics of Six Woody Species for Alley Cropping

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    Mosango, M.

    1999-01-01

    Full Text Available Leaves of six woody species (Leguminosae for alley cropping have been chemically analysed in order to evaluate their potentiality in the restoration of soil fertility. These species are : Acacia mangium, Cajanus cajan, Flemingia grahamiana, F. macrophylla, Leucaena leucocephala and Sesbania sesban. Nitrogen, carbon, cellulose, hemicellulose, lignin, active fraction and ash contents were determined as well as C/N and L/N ratios. AH these species appear to be rich in N and C. Fiber contents (cellulose, hemicellulose and lignin are globally low but variable from one species to another. C/N and L/N ratios are globally low. Among these species, Leucaena leucocephala and Senna spectabilis show the lowest C/N and LIN ratios. Such low values of C/N and L/N are normally found in species with rapid decomposition of organic matter.

  10. A PRELIMINARY STUDY ON FERMENTATION OF CANAVALIA ENSIFORMIS SEEDS

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    Indrawati Gandjar

    2012-09-01

    Full Text Available Pada umumnya di Indonesia kacang kedele difermentasi menjadi tempe kedele. Hasil fermentasi ini merupakan sumber protein dan vitamin yang penting dalam makanan penduduk sehari-hari. Di daerah-daerah tertentu di pulau Jawa beberapa jenis kacang-kacangan seperti Koro benguk (Mucuna pruriens, koro pedang (Canavalia ensiformis dan kacang hiris (Cajanus cajan juga difermentasi menjadi semacam tempe kedele. Dalam penelitian ini telah dilakukan fermentasi biji C. ensiformis menjadi tempe. Sejumlah kapang Rhizopus telah diperiksa kemampuannya untuk memfermentasikan biji-biji tersebut. R.oryzae R128 mampu menghasilkan tempe koro pedang dalam waktu 26 jam inkubasi pada suhu 30°C. Telah diteliti beberapa perubahan biokimia yang terjadi dalam substrat. Selama proses fermentasi berlangsung terjadi peningkatan dari kadar senyawa asam total, nitrogen amino dan karbohidrat terlarut. Analisa zat gizi dalam biji C. ensiformis mentah dan sesudah difermentasi menjadi tempe telah dilaku­kan.

  11. Antileishmanial and antifungal activity of plants used in traditional medicine in Brazil.

    Science.gov (United States)

    Braga, Fernanda G; Bouzada, Maria Lúcia M; Fabri, Rodrigo L; de O Matos, Magnum; Moreira, Francis O; Scio, Elita; Coimbra, Elaine S

    2007-05-01

    The antileishmanial and antifungal activity of 24 methanol extracts from 20 plants, all of them used in the Brazilian traditional medicine for the treatment of several infectious and inflammatory disorders, were evaluated against promastigotes forms of two species of Leishmania (L. amazonensis and L. chagasi) and two yeasts (Candida albicans and Cryptococcus neoformans). Among the 20 tested methanolic extracts, those of Vernonia polyanthes was the most active against L. amazonensis (IC(50) of 4 microg/ml), those of Ocimum gratissimum exhibited the best activity against L. chagasi (IC(50) of 71 microg/ml). Concerning antifungical activity, Schinus terebintifolius, O. gratissimum, Cajanus cajan, and Piper aduncum extracts were the most active against C. albicans (MIC of 1.25 mg/ml) whereas Bixa orellana, O. gratissimum and Syzygium cumini exhibited the best activity against C. neoformans (MIC of 0.078 mg/ml). PMID:17234373

  12. Fijación biológica de nitrógeno por tres fabáceas (Leguminosae) en suelos ácidos de Tabasco, México

    OpenAIRE

    S. Córdova-Sánchez; M. Castelán-Estrada; S. Salgado-García; J. D. Palma-López; J. A. Vera-Núñez; J. J. Peña-Cabriales; L. C. Lagunes-Espinoza; R. Cárdena-Navarro

    2011-01-01

    Con el objetivo de determinar el potencial de fijación biológica de nitrógeno por las fabáceas Cajanus cajan L., Canavalia ensiformis L. y Mucuna deerengiana L., en rotación y asociación con maíz durante dos ciclos de cultivo, se desarrollaron dos experimentos en un suelo ácido de la sabana de Huimanguillo, Tabasco. En ambos sistemas se evaluaron variables relacionadas con la nodulación de estas fabáceas, así como su efecto sobre el rendimiento de maíz (Zea mays L.); también se cuantificó ...

  13. Fijación biológica de nitrógeno por tres fabáceas (Leguminosae) en suelos ácidos de Tabasco, México

    OpenAIRE

    S. Córdova-Sánchez; M. Castelán-Estrada; S. Salgado-García; J. D. Palma-López; J. A. Vera-Núñez; J. J. Peña-Cabriales; L. C. Lagunes-Espinoza; R. Cárdena-Navarro

    2011-01-01

    Con el objetivo de determinar el potencial de fijación biológica de nitrógeno por las fabáceas Cajanus cajan L., Canavalia ensiformis L. y Mucuna deerengiana L., en rotación y asociación con maíz durante dos ciclos de cultivo, se desarrollaron dos experimentos en un suelo ácido de la sabana de Huimanguillo, Tabasco. En ambos sistemas se evaluaron variables relacionadas con la nodulación de estas fabáceas, así como su efecto sobre el rendimiento de maíz (Zea mays L.); también se cuantificó el ...

  14. Composição química de grãos de feijao-guandu cultivar kaki Chemical composition of pigeon pea seeds

    OpenAIRE

    João Paulo Feijão Teixeira; Dayse Soave Spoladore; Nelson Raimundo Braga; Eduardo Antonio Bulisani

    1985-01-01

    Foram analisadas sementes de tegumento creme e alaranjado de feijão-guandu (Cajanus cajan (L.) Milisp.), oriundas do cultivar Kaki, quanto aos teores de proteína, extrato etéreo, açúcares solúveis e polissacarídeos. Os dois tipos de sementes apresentaram composição química similar. Os teores médios encontrados foram: proteína 23,11%, extrato etéreo 1,34%, açúcar solúvel 9,46% e polissacarídeos 16,77%. A fração protéica apresentou baixos níveis de aminoácidos sulfurados, isoleucina, valina e t...

  15. Os sistemas agroflorestais como alternativa econômica em pequenas propriedades rurais: estudo de caso

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    Mário Jorge Santos Campos dos

    2002-01-01

    Full Text Available The Agroforestry Systems have been considered as an alternative approach for the stability of the ecosystems and in the same time promote the efficiency and optimization of the natural resources in the production in an integrated form. The objective of this case study is the economic evaluation of agroforestry systems in the Pontal do Paranapanema region. The Agroforestry System being studied is formed by agricultural species: corn (Zea maiz, guandu bean (Cajanus cajan, carioquinha, (Vigna unguiculata, black (Phaseolus vulgaris and cassava (Manihot esculenta with forestry species: Eucalyptus citriodora, Eucalyptus camaldulensis. The economic evaluation was based on economical criteria such as Net Present Value (NPL and Cost Benefict (C/B for the annual agricultural culture, Land Expectation Value (LET for the agroforestry component. The results showed that the agroforestry systems are economically acceptable to small farmers working in the same conditions presented in this study.

  16. Survey on pigeon pea production systems, utilization and marketing in semi-arid lands of Kenya

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    Baudoin J.P.

    2001-01-01

    Full Text Available In order to assess the status of pigeonpea (Cajanus cajan (L. Millsp. production in Kenya, two surveys were carried out in Makueni and Mbeere Districts in areas representative of the main agro-ecological pigeonpea producing zone of the country : (Mid-altitude ASAL. Participatory Rural Appraisal (PRA approach was chosen as research method and was completed by household interviews based on a semi-structured questionnaire. The main points developed are the presentation of the different farming systems in which pigeonpea is considered as an important legume crop, the identification of the factors explaining pigeonpea production variations, the quantification of the use of improved varieties and improved production practices, and the analysis of the major patterns and trends in pigeonpea production, consumption and marketing.

  17. Efeito de leguminosas nos atributos físicos e carbono orgânico de um Luvissolo Effect of legumes on the physical attributes and organic carbon of an Alfisol

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    João Tavares Nascimento

    2005-10-01

    Full Text Available A degradação de um solo sob cultivo é resultante, principalmente, de seu manejo inadequado, com reflexos nas baixas produtividades das culturas. Com a finalidade de avaliar o efeito de leguminosas nos atributos físicos e carbono orgânico (CO de um Luvissolo, realizou-se, no município de Alagoinha (PB, um experimento entre 1997 e 1999, com as seguintes espécies: crotalária (Crotalaria juncea, L, guandu (Cajanus cajan,L, guandu anão (Cajanus cajan (L Millsp, calopogônio (Calopogonium mucunoides, L, feijão-de-porco (Canavalia ensiforme, L, lab-lab (Dolichos lab lab, L, kudzu tropical (Pueraria phaseoloides, L, siratro (Macroptilium atropurpureum, L, leucena (Leucaena leucocephala, L, cunhã (Clitoria ternatea, L, mucuna preta (Styzolobium aterrimum, L, mucuna cinza (Styzolobium cinereum Piper e Tracy, e uma parcela com vegetação espontânea, como testemunha. O delineamento experimental utilizado foi o de blocos ao acaso em esquema de parcela subdividida, com distribuição de treze tratamentos principais na parcela (12 leguminosas + 1 testemunha e três tratamentos secundários, representados pelas profundidades de coleta de amostras de solo (0,00-0,10; 0,10-0,20; e 0,20-0,30 m, na subparcela, com três repetições. Na condição edafoclimática deste experimento, durante os três anos de trabalho, constatou-se que o emprego das leguminosas em relação à testemunha manteve inalterados o CO do solo, a densidade do solo, a porosidade total, a argila dispersa em água e a estabilidade dos agregados do solo.Soil degradation under cultivation results mainly from the inadequate management and it reflects in low crop yields. With the purpose of evaluating the effect of legumes on physical attributes and organic carbon content of an Alfisol, an experiment was conducted in the Alagoinha County-PB, Brazil, between 1997 and 1999, with the following species: Crotalaria juncea L, Cajanus cajan L, Cajanus cajan (L Millsp, Calopogonium mucunoides

  18. Adubação verde e alterações nas características químicas de um Cambissolo na região de Ji-Paraná-RO Green manure and changes on chemical characteristics of a soil in the Ji-Paraná-RO region

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    Elaine Almeida Delarmelinda

    2010-09-01

    Full Text Available A adubação verde promove benefícios nas características químicas, físicas e biológicas do solo. O trabalho avaliou o efeito da incorporação de diferentes adubos verdes nas características químicas de um Cambissolo háplico eutrófico. Foi utilizado o delineamento em blocos ao acaso, com oito tratamentos e três repetições. Os tratamentos consistiram das seguintes leguminosas: Calopogonium mucunoides, Crotalaria juncea, C. spectabilis, Cajanus cajan, Macrotyloma, Mucuna pruriens, Pueraria phaseoloides e a testemunha (sem leguminosa. As avaliações foram realizadas 60 dias após o corte e incorporação. A utilização de leguminosas como adubação verde proporcionou aumento nos teores de matéria orgânica, soma de bases e percentagem de saturação por bases, destacando-se a Pueraria phaseoloides, C. juncea e C. spectabilis.The green manure promotes benefits on the chemical, physical and biological properties of soil. The experiment evaluated the effect of incorporation of differents green manure on the chemistry characteristics of a soil. Experimental design used was a randomized block, with eight treatments and three replications. Treatments were: Calopogonium mucunoides, Crotalaria juncea, Crotalaria spectabilis, Cajanus cajan, Macrotyloma axillare, Mucuna pruriens and Pueraria phaseoloides and the treatment control (without leguminous. Evaluations of soil samplings were performed in 60 days after handling were performed. The results showed significant effects of the leguminous crop Pueraria phaseoloides, C. juncea and C. spectabilis on the fertility of the soil, with significant increments of organic matter, exchangeable bases and in the base saturation.

  19. Genome duplication and gene loss affect the evolution of heat shock transcription factor genes in legumes.

    Science.gov (United States)

    Lin, Yongxiang; Cheng, Ying; Jin, Jing; Jin, Xiaolei; Jiang, Haiyang; Yan, Hanwei; Cheng, Beijiu

    2014-01-01

    Whole-genome duplication events (polyploidy events) and gene loss events have played important roles in the evolution of legumes. Here we show that the vast majority of Hsf gene duplications resulted from whole genome duplication events rather than tandem duplication, and significant differences in gene retention exist between species. By searching for intraspecies gene colinearity (microsynteny) and dating the age distributions of duplicated genes, we found that genome duplications accounted for 42 of 46 Hsf-containing segments in Glycine max, while paired segments were rarely identified in Lotus japonicas, Medicago truncatula and Cajanus cajan. However, by comparing interspecies microsynteny, we determined that the great majority of Hsf-containing segments in Lotus japonicas, Medicago truncatula and Cajanus cajan show extensive conservation with the duplicated regions of Glycine max. These segments formed 17 groups of orthologous segments. These results suggest that these regions shared ancient genome duplication with Hsf genes in Glycine max, but more than half of the copies of these genes were lost. On the other hand, the Glycine max Hsf gene family retained approximately 75% and 84% of duplicated genes produced from the ancient genome duplication and recent Glycine-specific genome duplication, respectively. Continuous purifying selection has played a key role in the maintenance of Hsf genes in Glycine max. Expression analysis of the Hsf genes in Lotus japonicus revealed their putative involvement in multiple tissue-/developmental stages and responses to various abiotic stimuli. This study traces the evolution of Hsf genes in legume species and demonstrates that the rates of gene gain and loss are far from equilibrium in different species. PMID:25047803

  20. Produção de matéria seca e controle de plantas daninhas por leguminosas consorciadas com cana-de-açúcar em cultivo orgânico

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    Raíssa de Araujo Dantas

    2015-08-01

    Full Text Available Resumo:O objetivo deste trabalho foi avaliar a produção de matéria seca e o controle de plantas daninhas por espécies de leguminosas consorciadas com cana-de-açúcar, em cultivo orgânico. As espécies leguminosas Arachis pintoi, Cajanus cajan, Calopogonium mucunoides, Crotalaria anagyroides, Crotalaria juncea e Stylosanthes 'Campo Grande' foram semeadas em novembro de 2012, nas entrelinhas da cana-de-açúcar. O delineamento experimental em blocos ao acaso foi utilizado com quatro repetições e dois controles - um tratamento capinado e outro sem capinar. A massa de matéria seca das leguminosas foi avaliada aos 142 dias após a semeadura (DAS, e a massa de matéria seca de plantas daninhas, aos 29, 85 e 142 DAS. A massa de matéria seca de plantas daninhas foi inferior à da testemunha em todas as épocas amostradas. A produção de massa de matéria seca das leguminosas no consórcio foi baixa. As espécies Crotalaria juncea, Cajanus cajan e Calopogonium mucunoides produziram massa de matéria seca superior à das espécies Arachis pintoi e Stylosanthes 'Campo Grande'. A produtividade de colmos da cana-de-açúcar não foi afetada pelo consórcio. O consórcio entre cana-de-açúcar orgânica e leguminosas com semeadura tardia, para adubação verde, é eficiente no controle de plantas daninhas.

  1. Características fisiológicas e de crescimento de cafeeiro sombreado com guandu e cultivado a pleno sol Physiological characteristics and growth of coffee plants grown under shade of pigeonpea and unshaded

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    Heverly Morais

    2003-10-01

    Full Text Available O conhecimento dos efeitos do sombreamento sobre a fisiologia de cafeeiros é importante para se determinar níveis ótimos de radiação e temperatura, bem como para subsidiar estudos sobre o crescimento de plantas sombreadas, a fim de determinar a arquitetura ideal do cafeeiro que maximize a captura da radiação solar disponível em ambientes sombreados. O objetivo deste trabalho foi avaliar características fisiológicas e de crescimento de cafeeiros (Coffea arabica L. cultivados sob sombreamento denso com guandu (Cajanus cajan (L. Millsp. e a pleno sol. O baixo nível de radiação incidente sobre os cafeeiros sombreados com guandu resultou em decréscimos na taxa fotossintética e na transpiração, maior altura de planta, folhas maiores e com menor quantidade de matéria seca. Esses resultados indicam que o excesso de sombra afeta drasticamente a fisiologia e morfologia de C. arabica.The characterization of shade effects on the physiology of coffee is important to determine optimum levels of radiation and temperature, as well as to subsidize studies on growth of shaded plants aiming at determining the ideal coffee plant architecture that maximizes the capture of the available solar radiation in shaded environments. The objective of this work was to evaluate physiological and growth characteristics of coffee (Coffea arabica L. shaded with pigeonpea (Cajanus cajan (L. Millsp. and under full sun. The low level of incident radiation on the coffee shaded with pigeonpea caused decreases in the photosynthetic rate and transpiration, increased plant height and leaf size, but diminished leaf dry matter. These results indicate that the excess of shade drastically affects the physiology and morphology of C. arabica.

  2. Economic analysis of rice and common bean production in succession to green manure crops and mecahnical soil decompression in Brazilian Savannah

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    Vagner do Nascimento

    2016-06-01

    Full Text Available ABSTRACT The previous cultivation of green manures and mechanical soil decompression are options to minimize compaction of the topsoil in no-tilage system (NTS set in different production systems in the Brazilian Savannah. In addition, it is essential to relate these agricultural practices with the economic benefits generated through the production cycles. The objective of this study was to evaluate economically the effect of sporadic mechanical decompression of the soil and previous cultivation of green manure in the production and net gain margin of upland rice and "winter" common bean, under sprinkler irrigation, in NTS in lowland Brazilian savannah. This study was developed in the 2012/13 harvest and 2013 winter in Selvíria, state of Mato Grosso do Sul, in an clay texture Oxisol in the savanah in the state of Mato Grosso do Sul, in a randomized block design arranged in a 5 x 2 factorial arrangement with four replications. The treatments were a combination of five green manures (fallow (control, Cajanus cajan, Crotalaria juncea, Pennisetum glaucum and Urochloa ruziziensis with and without mechanical soil scarification. The yields of upland rice and common bean grains were not influenced by the previous green manure cultivation; the upland rice grown in succession to Cajanus cajan in the presence of mechanical soil scarification provided greater increase in grain yield and higher gross margin profit. Beans cultivated in succession to Crotalaria juncea and Pennisetum glaucum in the presence of mechanical soil scarification, followed by rice cultivation, provided greater increases in grain yield and gross profit margins.

  3. Occurence and host specificity of indigenous rhizobia from soils of São Paulo State, Brazil Ocorrência de rizóbios nativos em plantas hospedeiras de solos nativos do Estado de São Paulo, Brasil

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    Maria Luiza Colognesi de Oliveira Lombardi

    2009-08-01

    Full Text Available The occurrence of rhizobial communities at four sites under natural vegetation and one site under pasture were examined. Isolates of rhizobia originating from crotalaria (C. junceae, common bean (Phaseolus vulgaris and pigeon pea (Cajanus cajan were studied in relation to population density, host specificity and the interaction between rhizobial occurrence, climatic conditions and soil properties. pH values and potential acidity were the soil properties that most affected rhizobial occurrence. Rhizobia from crotalaria and common bean were evaluated at four sites, and from pigeon pea, at five sites. Common bean was the most specific legume, followed by peanuts, crotalaria and pigeon pea.Foi examinada a ocorrência de comunidades de rizóbios em quatro locais de vegetação natural e um local de pastagem. Isolados de rizóbio originados de crotalária (C. junceae, feijão (Phaseolus vulgaris e guandu (Cajanus cajan foram estudados em relação à densidade populacional, planta hospedeira e interação entre ocorrência de rizóbio, condições climáticas e propriedades do solo. Os valores de pH e potencial de acidez foram as propriedades do solo que mais contribuíram para a ocorrência de rizóbio. A ocorrência de rizóbio em crotalária e feijão foi estudada em quatro locais, e em guandu em cinco locais. O feijão foi mais específico, seguido por crotalária e guandu.

  4. Degradabilidade ruminal da matéria seca e da proteína bruta de folhas e folíolos de forrageiras tropicais Ruminal degradabilities of dry matter and crude protein of tropical forages

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    Cristina Mattos Veloso

    2006-04-01

    Full Text Available Foram determinadas as degradabilidades in situ da MS e PB dos folíolos de três leguminosas tropicais leucena (Leucaena leucocephala (Lam. de Wit, guandu (Cajanus cajan (L. Millsp. e soja perene (Neonotonia wightii e de folhas de outras duas forrageiras tropicais rami (Boehmeria nivea (L. Gaudich e mandioca (Manihot esculenta Crantz. Amostras de 3 g (guandu, soja e rami ou 5 g (leucena e mandioca do material foram incubadas no rúmen de quatro novilhos por períodos de 3, 6, 12, 18, 24 e 48 horas. As degradabilidades efetivas da MS e da PB para a taxa de passagem de 5% hora foram elevadas (acima de 60%. As taxas de degradação, exceto a do guandu, também foram consideradas elevadas. As forrageiras apresentaram alta digestibilidade total da proteína (acima de 88%, exceto os folíolos de guandu (69%. O guandu foi a forrageira com menor potencial de degradação da proteína.In situ DM and CP degradabilities of leaflets of three tropical legumes: leucaena (Leucaena leucocephala (Lam. de Wit, pigeon pea (Cajanus cajan (L. Millsp., and glycine (Neonotonia wightii and of leaves of two other tropical forages: ramie (Boehmeria nivea (L. Gaudich and cassava (Manihot esculenta Crantz were determined in this trial. Three grams of pigeon pea, glycine, and ramie and 5 g of leucaena and cassava were incubated in the rumen of four steers for 3, 6, 12, 18, 24, and 48 hours. Effective degradabilities of DM and CP assuming a passage rate of 5%/hour were greater than 60%. Degradation rates, except for pigeon pea, were also high in this study. Forages had high total digestibility of protein (greater than 88%, except for pigeon pea leaflets that was 69%. Pigeon pea was the forage with the lowest potential for ruminal protein degradation.

  5. Comportamento de Sternechus subsignatus (Boheman em dez espécies vegetais de verão para rotação de culturas ou cultura armadilha no plantio direto Sternechus subsignatus (Boheman behaviour in ten summer rotation specles and trapping crops for no-tillage system

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    Mauro Tadeu Braga da Silva

    1997-12-01

    Full Text Available O desenvolvimento de Sternechus subsignatus (Boheman (Coleoptera: Curculionidae foi avaliado em dez espécies vegetais sob condições naturais num solo manejado há três anos no sistema de plantio direto corn soja cultivada no verão. Foram observados o número de plantas atacadas, a oviposição, o núrnero e peso de larvas, o número de larvas hibernantes no solo e o número de adultos emergidos. O inseto se reproduziu e desenvolveu ern feijão (Phaseolus vulgaris L guandu anão (Cajanus cajan L, lab-lab (Dolichos lablab L. e soja (Glycine max L. e ao contrário, em crotalária júncea (Crotalaria júncea L. girassol (Helianthus annuus L., milheto (Pennisetum americanum Leek, milho (Zea mays L, mucuna preta (Stizolobium aterrimum Piper et Tracy e sorgo (Sorghum bicolor L. não completou o ciclo de vida. Os resultados indicaram que a cultura do feijão, guandu anão, lab-lab e soja aumentaram a população do inseto. Entretanto, ocorreu redução na população do inseto corn as culturas da crotalária júncea, girassol, milheto, milho, mucuna preta e sorgo. As quatro primeiras espécies são hospedeiras preferenciais, podendo ser usadas como culturas armadilhas e as demais, não são hospedeiras, pois propiciam a diminuição da população do inseto, sendo ideais para uso em sistemas derotação de culturas no verão, em áreas infestadas, para substituir o monocultivo da soja em plantio direto.The development of Sternechus subsignatus (Boheman (Coleoptera. Curculionidae was evaluated on ten crop species under natural conditions under no-tillage system for three years and infested with the insect Soybean was the Summer crop continously cultivated in the area. Number of attacked plants, oviposition, number and weight of larvae and number of adults emerged were the parameters evaluated. The insect reproduced and developed in bean (Phaseolus vulgaris L, grandull (Cajanus cajan L., dolichos (Dolichos lablab L. and soybean (Glycine max L. and

  6. Recuperação do subsolo em área de empréstimo usada para construção de hidrelétrica Subsoil reclamation in loan area used for hydroelectric construction

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    Marlene Cristina Alves

    2011-06-01

    , degraded by soil removal for hydroelectric power plant construction, using green manure and applying lime and gypsum. A randomized block design was used with seven treatments and four replications. The research was started in 1992 and the treatments were: Control; Cajanus cajan until 1994 followed by Canavalia ensiformis; lime + Stizolobium aterrimum; lime + Cajanus cajan until 1994 and followed by Canavalia ensiformis; lime + gypsum + Stizolobium aterrimum and lime + gypsum + Cajanus cajan until 1994 and followed by Canavalia ensiformis. The treatments consisted of green manure, lime and gypsum. Seven years later, in 1999, the attributes analyzed were: total soil porosity, macroporosity, microporosity, soil bulk density, organic matter and brachiaria dry matter production. The treatments are contributing to the recovery of physical attributes of exposed subsoil, and the treatment with Stizolobium aterrimum without soil correction seems to be the most promising. Soil recuperation effects can be seen on the 0.00-0.10 m layer.

  7. Produção de fitomassa de diferentes espécies de cobertura e suas alterações na atividade microbiana de solo de cerrado Phytomass yield of different cover crops and alterations in the microbial activity in a cerrado soil in Brazil

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    Marco Aurélio Carbone Carneiro

    2008-01-01

    Full Text Available O objetivo deste estudo foi avaliar a produção de fitomassa de nove espécies de cobertura do solo e verificar as alterações na atividade e biomassa microbiana de solo de cerrado. O trabalho foi desenvolvido de março a dezembro de 2004, em Latossolo Vermelho distroférrico, sendo semeadas as seguintes espécies de cobertura: aveia-preta (Avena strigosa, guandu (Cajanus cajan, Crotalaria juncea, lablabe (Lablab purpureum, milheto (Pennicetum americanum, nabo forrageiro (Raphanus sativus, níger (Guizotia abyssinica (L.F. Cass., feijão-de-porco (Canavalia ensiformis. e Crotalatia spectabilis. Uma área em pousio foi considerada como referência. Na ocasião do florescimento, determinou-se a quantidade de fitomassa e, a partir de agosto, parte dela foi acondicionada em sacos de polietileno, ficando no solo por 120 dias, para se avaliar a razão de decomposição. Durante esse período, no inicio de cada mês, foram avaliados o carbono da biomassa microbiana e a respiração microbiana do solo. As espécies mais promissoras para produção de fitomassa foram o guandu, milheto, feijão-de-porco e niger. O carbono da biomassa microbiana foi negativamente afetado nas áreas com resíduos de milheto, nabo forrageiro, crotalária espectábilis e naquela em pousio. Na área sob pousio, verificou-se a menor produção de fitomassa, além da redução no carbono da biomassa microbiana e mais perda de carbono, sendo esta prática não indicada para a região de cerrado.The objective of this study was to evaluate the phytomass yield of nine species of cover crops of the soil and to verify the alterations in the activity and microbial biomass of Cerrado soil, in the State of Goiás, Brazil. The work was developed from March to December, 2004, being sowed the following covering species: Avena strigosa, Cajanus cajan, Crotalaria juncea, Lablab purpureum, Pennicetum americanum, Raphanus sativus, Guizotia abyssinica, Canavalia ensiformis, Crotalatia

  8. Indicadores biológicos associados ao ciclo do fósforo em solos de Cerrado sob plantio direto e plantio convencional Soil biological indicators associated to the phosphorus cycle in a Cerrado soil under no-till and conventional tillage systems

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    Roberto Guimarães Carneiro

    2004-07-01

    Full Text Available O objetivo deste trabalho foi avaliar a influência do plantio direto, do preparo convencional do solo e de plantas de cobertura sobre indicadores biológicos associados ao ciclo do fósforo (P. O trabalho foi realizado em três áreas adjacentes, num Latossolo Vermelho-Amarelo, distribuídas da seguinte maneira: área I, dois anos de experimentação, comparando esses sistemas de plantio; área II, seis anos de experimentação e área III, Cerrado nativo. Em julho/1998 e janeiro/1999, avaliaram-se: o P da biomassa, populações de fungos e bactérias totais e solubilizadores de fosfato e a atividade da fosfatase ácida, nas profundidades de 0-5 e de 5-20 cm. A atividade da fosfatase e a ocorrência de fungos e bactérias solubilizadoras nas áreas com plantio direto foram significativamente superiores às das áreas com plantio convencional, na profundidade de 0-5 cm. O tempo de implantação acentuou as diferenças entre plantio direto e convencional quanto aos indicadores avaliados. O P da biomassa diminuiu no tratamento plantio direto com nabo forrageiro (Raphanus sativus da área II. Os fungos solubilizadores aumentaram na presença de guandu (Cajanus cajan e nabo forrageiro, e as bactérias solubilizadoras aumentaram na presença de guandu. O solo sob vegetação nativa apresentou maior atividade da fosfatase em relação ao das áreas cultivadas, evidenciando a importância da mineralização do P orgânico nesse ecossistema.The objective of this work was to evaluate the effects of no-till, conventional tillage and cover crops on biological indicators associated to the P cycle. The work was carried out on three adjacent areas on a Red-Yellow Oxisol: area I, a two-year experiment comparing the two management systems; area II, a six-year experiment, and area III, a native Cerrado vegetation. The soil was sampled at two depths (0-5 cm and 5-20 cm in July/1998 and January/1999. The biological indicators evaluated were microbial biomass P

  9. Fitorremediação do herbicida trifloxysulfuron sodium Phytoremediation of the herbicide trifloxysulfuron sodium

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    J.B. Santos

    2004-06-01

    Full Text Available Este trabalho teve como objetivo avaliar a eficiência de espécies vegetais na fitorremediação do herbicida trifloxysulfuron sodium em solos, utilizando o milho como planta indicadora. Os tratamentos foram compostos pela combinação de espécies (Calopogonium muconoides, Crotalaria juncea, Crotalaria spectabilis, Vicia sativa, Cajanus cajan, Canavalia ensiformis, Helianthus annus, Dolichus lablab, Pennisetum glaucum, Stylosantes guianensis, Mucuna deeringiana, Mucuna cinereum, Mucuna aterrima, Raphanus sativus e Lupinus albus, semeadas anteriormente à cultura do milho, mais um tratamento controle (sem cultivo prévio e três doses do herbicida trifloxysulfuron sodium (0,00; 3,75; e 15,00 g ha-1. O delineamento experimental usado foi o de blocos ao acaso, em esquema fatorial, sendo utilizadas três repetições. A semeadura das espécies vegetais nos vasos foi feita no dia seguinte à aplicação do trifloxysulfuron sodium. Após 80 dias da semeadura, as espécies vegetais foram cortadas na altura do coleto, descartando-se a sua parte aérea. A seguir, foi realizada a semeadura do milho (cultivar AG-122. Aos 45 dias após a emergência das plantas de milho foram avaliadas a altura de plantas e a sua biomassa seca da parte aérea. Verificou-se que as espécies M. aterrima e C. ensiformis foram eficientes na descontaminação do herbicida trifloxysulfuron sodium em solo.This work aimed to evaluate the efficiency of plant species in the phytoremediation of the herbicide trifloxysulfuron sodium in soils, by using corn crop as a bioindicator. The treatments were composed by the combination of the species (Calopogonium muconoides, Crotalaria juncea, Crotalaria spectabilis, Vicia sativa, Cajanus cajan, Canavalia ensiformis, Helianthus annus, Dolichus lablab, Pennisetum glaucum, Stylosantes guianensis, Mucuna deeringiana, Mucuna cinereum, Mucuna aterrima, Raphanus sativus and Lupinus albus sowed before the corn, without previous cultivation (control

  10. Potencial de espécies vegetais para a remediação do herbicida trifloxysulfuron-sodium Potential of plant species for remediation of trifloxysulfuron-sodium

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    S.O. Procópio

    2005-03-01

    Full Text Available Este trabalho teve como objetivo avaliar a eficiência de espécies vegetais na remediação do herbicida trifloxysulfuron-sodium em solos, utilizando o feijão (Phaseolus vulgaris como planta indicadora. Os tratamentos foram compostos pela combinação entre as espécies Calopogonium mucunoides, Crotalaria juncea, Crotalaria spectabilis, Vicia sativa, Cajanus cajan, Canavalia ensiformis, Medicago sativa, Dolichus lab lab, Penisetum glaucum, Stylosantes guianensis, Mucuna deeringiana, Mucuna cinereum, Mucuna aterrima, Raphanus sativus e Lupinus albus. Todas as espécies foram semeadas em vasos no dia seguinte à aplicação do trifloxysulfuron-sodium em três doses (0,00; 3,75; e 15,00 g ha-1. Após 80 dias da semeadura, as espécies vegetais foram cortadas na altura do coleto e a parte aérea destas descartada. A seguir, foi realizada a semeadura do feijão (cultivar Pérola. Aos 45 dias após a emergência das plantas de feijão avaliaram-se a altura e a massa seca da parte aérea das plantas. Melhor eficiência na descontaminação do trifloxysulfuron-sodium em solo foi obtida pelas espécies M. aterrima e C. ensiformis.This work aimed to evaluate the efficiency of vegetable species in the remediation of the herbicide trifloxysulfuron-sodium in soils using the common bean (Phaseolus vulgaris as a bio indicator. The treatments were composed by the combination of the species Calopogonium mucunoides,Crotalaria juncea, Crotalaria spectabilis, Vicia sativa, Cajanus cajan, Canavalia ensiformis, Medicago sativa, Dolichus lab lab, Penisetum glaucum, Stylosantes guianensis, Mucuna deeringiana, Mucuna cinereum, Mucuna aterrima, Raphanus sativus and Lupinus albus, sown in vases the day following application of the herbicide at three doses (0.00; 3.75; and 15.00 g ha-1. Eighty days after sowing, the plants were cut and the shoot discarded, followed by bean ("Perola" cultivar sowing. At 45 days post emergence, bean height and shoot dry matter were

  11. Produção de biomassa e teor de macronutrientes do milheto, feijão-de-porco e guandu-anão em cultivo solteiro e consorciado Biomass production and macronutrients quantity of millet, jack bean, guandu single and in intercropping

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    Cícero Monti Teixeira

    2005-02-01

    Full Text Available Objetivou-se determinar a produção de biomassa e o teor de macronutrientes do milheto (Pennisetum typhoides (Burm. Stapf, feijão-de-porco (Canavalia ensiformes (L. DC. e guandu-anão (Cajanus cajan (L. Millsp. em cultivo solteiro e nos consórcios da gramínea com as leguminosas, visando a produção de palha no sistema plantio direto. O trabalho foi conduzido no campo experimental do Departamento de Agricultura da Universidade Federal de Lavras (Lavras, MG, Brasil. O delineamento foi o de blocos casualizados, com cinco tratamentos e quatro repetições. Apenas o guandu-anão solteiro apresentou menor produção de fitomassa fresca e seca, sendo que os demais tratamentos não diferiram entre si, pelo teste de Tukey a 5% de probabilidade. De forma geral, as leguminosas apresentaram maiores teores de N e Ca e o milheto maiores teores de S. Apenas o feijão-de-porco solteiro apresentou menor teor de P. Os maiores teores de K foram apresentados pelo milheto solteiro e consorciado com as leguminosas e pelo feijão-de-porco em consórcio com milheto. Os menores teores de Mg foram verificados para o guandu-anão.The object of this work was to evaluate the biomass production and the macronutrients quantity of millet (Pennisetum typhoides (Burm. Stapf, jack bean (Canavalia ensiformes (L. DC., guandu (Cajanus cajan, (L. Millsp. dwarf cultivar, and the millet with leguminous consortiums to straw production in no-tillage system. The experiment was carried in experimental area at Agriculture Department of the Federal University of Lavras (Lavras, Minas Gerais state, Brazil. The experimental design was randomized blocks, with five treatments and four replications. For biomass production, only the guandu presented smaller fresh and dry biomass yield than the other treatments. In general, the leguminous presented the greatest N and Ca quantities and millet the greatest quantities of S. Only jack bean presented minor P quantity. For K the greatest quantities

  12. Potencial de espécies utilizadas como adubo verde no manejo integrado de plantas daninhas Potential of species used as green manure in the integrated weed management

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    E.A.L. Erasmo

    2004-09-01

    Full Text Available O presente trabalho foi conduzido na Estação Experimental da Universidade Federal do Tocantins, Gurupi-TO, Brasil. O experimento foi instalado com o objetivo de avaliar durante 60 dias, em campo, a interferência de oito espécies utilizadas freqüentemente como adubos verdes (Mucuna aterrima, Mucuna pruriens, Crotalaria ochroleuca, Crotalaria spectabilis, Canavalia ensiformis, Cajanus cajan, Pennisetum americanum e Sorghum bicolor, híbrido BR304 sobre a comunidade infestante. As espécies de plantas daninhas mais freqüentes na área do experimento foram: Digitaria horizontalis, Hyptis lophanta e Amaranthus spinosus. Foram realizadas amostragens aos 15, 30, 45 e 60 dias após a formação da cobertura, utilizando um quadrado de amostragem equivalente a 0,25 m². As plantas daninhas foram devidamente identificadas, coletadas, secas e pesadas. O delineamento experimental utilizado foi inteiramente casualizado e constou de nove tratamentos, com quatro repetições cada. Verificou-se que as espécies C. spectabilis, S. bicolor, C. ochroleuca, M. aterrima e M. pruriens reduziram significativamente o número e o peso da matéria seca da população das plantas daninhas avaliadas (D. horizontalis, H. lophanta e A. Spinosus, principalmente as duas últimas, enquanto P. americanum mostrou-se a menos eficiente nesse aspecto.This work was carried out at the Experimental Station of the University of Tocantins, Gurupi-TO, Brazil, to evaluate the interference of eight species frequently used as green manure (Mucuna aterrima, Mucuna pruriens, Crotalaria ochroleuca, Crotalaria spectabilis, Canavalia ensiformis, Cajanus cajan, Pennisetum americanum and Sorghum bicolor, hybrid BR304 in the weed community, for sixty days under field conditions. The most frequent weed species in the experimental area were Digitaria horizontalis, Hyptis lophanta and Amaranthus spinosus. Samplings were made at 15, 30, 45 and 60 days after formation of green manure covering, using

  13. Abelhas (Hymenoptera: Apoidea visitantes das flores do feijão guandu no Recôncavo Baiano, Brasil Bees (Hymenoptera: Apoidea on pigeonpea flowers in 'Recôncavo Baiano' region, Brazil

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    Ruberval Leone Azevedo

    2007-10-01

    Full Text Available O feijão guandu Cajanus cajan L. é uma cultura comum, encontrada com freqüência em todo o Brasil, principalmente em pequenas propriedades rurais. Este trabalho teve por objetivo obter informações sobre a diversidade de abelhas visitantes das flores do feijão guandu, gerando informações sobre a estrutura da comunidade de abelhas e a importância desta leguminosa como fonte de recursos tróficos. As coletas foram feitas semanalmente, entre maio e outubro de 2005, na área experimental de Entomologia do Centro de Ciências Agrárias da UFBA, localizada no município de Cruz das Almas, Bahia, no intervalo entre as 6:00 e as 18:00 horas. Um total de 4.676 indivíduos foi coletado visitando as flores do feijão guandu. A família Apidae foi representada por 99,3% dos indivíduos e 76,2% das espécies amostradas. Os gêneros Xylocopa e Trigona foram os que apresentaram maior riqueza, com quatro e três espécies, respectivamente. Trigona spinipes foi a espécie mais abundante, com freqüência relativa igual a 83,1%, seguida de Nannotrigona testaceicornis (5,1%.Pigeonpea, Cajanus cajan L. is a common crop, frequently found throughout Brazil, mainly in small rural properties. This research was aimed at geting information on the diversity of visiting bees to flowers of pigeonpea, generating information on the structure of the bee community and the importance of this Leguminosae as source of food resources. Collections had been made weekly, between May and October 2005 in the Experimental area of the Center of Agrarian Sciences and Environmental of the UFBA, located in the Cruz das Almas, Bahia, in the interval between 6:00 AM and 06:00 PM. A total of 4.676 individuals was collected visiting the pigeonpea flowers. The Apidae family was represented by 99.25% of individuals and 76.19% of total species. The genera Xylocopa and Trigona were the ones that showed greater richness, with four and three species, respectively. Trigona spinipes was the most

  14. Especificidade hospedeira de variantes Bradyrhizobium spp em soja (cvs peking e clark, caupi e guandu Host specificity of Bradyrhizobium spp mutants in soybean (cultivars peking and clark, cowpea and pigeon pea

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    Fabíola Gomes de Carvalho

    2008-12-01

    Full Text Available A expansão da cultura da soja evidenciou uma alta especificidade hospedeira, requerendo a pesquisa de novas estirpes que apresentassem capacidade de nodular a soja e bom potencial de competição com a população de rizóbios naturalizada nos solos. O objetivo deste trabalho foi avaliar a especificidade hospedeira de variantes isolados de estirpes de Bradyrhizobium spp quanto à nodulação e eficiência relativa de fixação de N2 atmosférico em soja (cvs peking e clark, caupi e guandu. O experimento foi realizado sob condições controladas em câmara de crescimento por meio de testes de variantes de B. japonicum e B. elkanii e suas respectivas estirpes originais quanto à habilidade de nodular soja, caupi e guandu. A colheita foi realizada aos 35 dias, sendo avaliada a nodulação (número, peso dos nódulos secos, produção de matéria seca na parte aérea, eficiência relativa de fixação de N2 atmosférico. Os variantes e estirpes de Bradyrhizobium spp nodularam Glycine max (cultivares BR-16, Clark e Peking, Vigna unguiculata e Cajanus cajan, contudo, apenas para Glycine max a interação rizóbio-leguminosa demonstrou eficiência simbiótica significativa.The expansion of the soybean crop has evidenced a high host specificity, indicating the requirement of new strains with capacity of nodulating soybean and competition capacity with the natural soil rhizobial population. The purpose of this study was to evaluate the host specificity of mutants isolated from Bradyrhizobium spp strains in relation to nodulation and relative N2 fixation effectiveness in soybean (cultivars Peking and Clark, cowpea and pigeon pea. The experiment was carried out under controlled conditions in a growth chamber, where B. japonicum and B. elkanii mutants and the respective original strains were tested for their nodulation ability on soybean, cowpea and pigeon pea. The crop was harvested 35 days after planting and the nodulation (number, dry nodule weight

  15. BANCO DE SEMENTES DE PLANTAS DANINHAS EM SOLO CULTIVADO COM ADUBOS VERDES WEED SEED BANK IN GREEN-MANURE-CULTIVATED FIELDS

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    FRANCISCO JOSÉ SEVERINO

    2001-01-01

    Full Text Available Com o objetivo de estudar a composição de banco de sementes de plantas daninhas em solo cultivado com adubos verdes, desenvolveu-se um experimento em casa de vegetação do Departamento de Produção Vegetal da Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP, Piracicaba (SP, entre dezembro de 1999 e fevereiro de 2000. As parcelas experimentais, compostas de 1 kg de solo amostrado por um trado de 4,3 cm de diâmetro, a partir da experimentação em campo, foram instaladas em bandejas de 30 x 20 x 5 cm. O delineamento experimental adotado foi o de blocos ao acaso com quatro repetições constando dos fatores: a adubos verdes - amendoim-forrageiro (Arachis pintoi Krapov. & W.C. Gregory, crotalária (Crotalaria juncea L., guandu-anão [Cajanus cajan (L. Millsp.] e testemunha sem capina; b plantas daninhas - capim-braquiária (Brachiaria decumbens Stapf., capim-colonião (Panicum maximum Jacq., picão-preto (Bidens pilosa L., além da vegetação espontânea. Avaliou-se o número de plantas daninhas emergidas aos 30 e 60 dias após a instalação do experimento. Os resultados permitem concluir que o banco de sementes de plantas daninhas foi significativamente reduzido com a utilização dos adubos verdes, constituindo em prática agrícola relevante para o manejo integrado de plantas daninhas.In order to evaluate the composition of a weed seed bank in a soil cultivated with green manure, an experiment was carried out from December, 1999, to February, 2000, in a greenhouse of the Plant Production Department, ESALQ/University of São Paulo, Piracicaba, Brazil. The experimental units were made of 30 x 20 x 5 cm trays, filled with 1.0 kg of soil. The soil was collected by a 4.3 cm core sampler from the experimental field where the green manure and weeds were grown. The experiment had a completely randomized block design with four replicates, green manure (Arachis pintoi Krapov. & W.C. Gregory, Crotalaria juncea L. and Cajanus cajan L

  16. Effect of plant and fungous metabolites on Meloidogyne exigua Efeito de metabólitos vegetais e fúngicos sobre Meloidogyne exigua

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    Daniel Rufino Amaral

    2009-01-01

    Full Text Available As nematodes cause great damage to Brazilian coffee production, effective methods to control these parasites are necessary. In a previous work Allium cepa L., Cajanus cajan (L. Mill., Crotalaria juncea L., Ficus elastica Roxb., Ruta graveolens L., Stylosanthes guianensis Aubl., Leucaena leucocephala (Lam. Dewit., Brachiaria decumbens Stapf., Catharanthus roseus G. Don, Tagetes minuta L., Ricinus communis L. and Coffea arabica L. produced active substances against Meloidogyne exigua Goeldi, a nematode widely disseminated through Brazilian coffee fields. Thus, aqueous extracts of such plants, collected in a different season from that of the previous work, as well as crude metabolites produced in liquid medium by Fusarium moniliforme Shelden and Cylindrocarpon magnusianum (Sacc. Woll., were submitted to in vitro assays with M. exigua second-stage juveniles (J2. All plants and fungi produced active substances against J2. Therefore, their metabolites were applied to six-month-old coffee plants inoculated with M. exigua. After 90 days in a greenhouse, those samples obtained from A. cepa, L. leucocephala, R. graveolens and F. moniliforme inhibited the production of galls and eggs by M. exigua, demonstrating potential to control such parasite.Os nematóides acarretam grandes perdas aos produtores brasileiros de café, sendo necessário o desenvolvimento de métodos eficientes para o seu controle. Em trabalho anterior, Allium cepa L., Cajanus cajan (L. Mill., Crotalaria juncea L., Ficus elastica Roxb., Ruta graveolens L., Stylosanthes guianensis Aubl., Leucaena leucocephala (Lam. Dewit., Brachiaria decumbens Stapf., Catharanthus roseus G. Don, Tagetes minuta L., Ricinus communis L. e Coffea arabica L. produziram substâncias ativas contra o nematóide Meloidogyne exigua Goeldi, que é amplamente disseminado pelos cafezais brasileiros. Dando continuidade a esse trabalho, extratos aquosos das plantas mencionadas, coletadas em época diferente daquela

  17. Impacto do manejo nos atributos físicohídricos de um Latossolo Vermelho sob cerrado, no município de Selvíria, Estado do Mato Grosso do Sul = The impact of management on the physical and hydrological attributes of a darkred latosol under cerrado in Selviria, State of Mato Grosso do Sul

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    Monica Martins da Silva

    2006-01-01

    Full Text Available Verificou se a influência da semeadura direta, convencional e quatro plantas de cobertura na condutividade hidráulica saturada, infiltração, retenção, armazenamento de água e potencial mátrico em um Latossolo Vermelho de cerrado cultivado com milho, na Unesp deIlha Solteira, Estado de São Paulo. Utilizouse o delineamento experimental de blocos casualizados, no esquema em faixas com parcelas subdivididas, com 4 blocos. As parcelas foram constituídas pelas plantas de cobertura: Mucuna aterrima, Pennisetum americanum,Crotalaria juncea e Cajanus cajan e área de pousio, e as subparcelas pelos sistemas de semeadura direta (SD e convencional (SC. Nas camadas de 0,00,10 e 0,100,20 m nas tensões entre 6 e 100 kPa, a SC obteve maior armazenamento de água no solo; apesar da SC (0 0,20m apresentar maior armazenamento de água, comparado à SD, o mesmo foi menos eficiente em disponibilizar água às plantas; a SD apresentou maior uniformidade de distribuição de água no perfil do solo.This research work studied the influence of notillage, conventional tillage and four ground cover plants on the saturated hydraulic conductivity, infiltration, retention, storage of water and matricpotential in a DARKRED LATOSOL under cerrado, cultivated with maize. The study was conducted at Unesp of Ilha Solteira, State of São Paulo. A completely randomized design was used with subdivided parcels (randomized block design, with five treatments and fourrepetitions. The parcels were constituted of the following ground cover plants: Mucuna aterrima, Pennisetum americanum, Crotalaria juncea, Cajanus cajan and an area of spontaneous vegetation. As for the parcels subdivisions, the systems of soil management implemented were notillage (NT and conventional tillage (CT. In the layers of 0.00.10 and 0.100.20 m, for tensions between 6 and 100 kPa, the CT system made the soil achieve greater water storage. Although CT (0 – 0.20 m presented greater water storage compared

  18. Degradabilidade ruminal do feno de alguns alimentos volumosos para ruminantes Ruminal degradability of some roughage hays for ruminants feeding

    Directory of Open Access Journals (Sweden)

    G.G.P. Carvalho

    2006-08-01

    Full Text Available Avaliou-se a degradabilidade ruminal da matéria seca (MS, proteína bruta (PB, fibra em detergente neutro (FDN, da fibra em detergente ácido (FDA e hemicelulose dos fenos de capim-elefante (Pennisetum purpureum, palma (Opuntia ficus, guandu (Cajanus cajan e parte aérea da mandioca (Manihot esculenta utilizando três bovinos mestiços machos, castrados, canulados no rúmen e mantidos em regime de pasto. Amostras de 4g de cada alimento foram incubadas em duplicata no rúmen dos animais, nos períodos de 0, 6, 12, 24, 36, 48 e 72 horas. A degradabilidade potencial da PB dos fenos de capim-elefante e guandu foi semelhante, 83,9 e 81,2%, respectivamente. Os maiores valores foram observados para os fenos de palma (94,2% e parte aérea da mandioca (91,7%. A degradabilidade efetiva (DE foi obtida considerando as taxas de passagem de 2, 5 e 8%/hora. A maior DE observada para MS (60,5%, PB (81,1%, FDN (21,6%, FDA (27,9% e HEM (58,0%, na taxa de passagem de 5%/h, ocorreu com o feno de palma.The ruminal degradability of dry matter (DM, crude protein (CP, neutral detergent fiber (NDF, acid detergent fiber (ADF and hemicellulose (HEM of elephantgrass (Pennisetum purpureum, forage cactus (Opuntia ficus, pigeon pea (Cajanus cajan and cassava foliage (Manihot esculenta hays was evaluated using three cannulated crossbred steers, kept on pasture. Samples of four grams of each hay were incubated in the rumen for 0, 6, 12, 24, 36, 48 and 72 hours. The CP potential degradability (PD for elephantgrass and pigeon pea hays was similar, 83.9 and 81.2%, respectively. Higher values were observed either for forage cactus (94.2% or cassava foliage (91.7% hays. The effective degradability (ED was obtained considering the passage rates of 2, 5 and 8%/hour. The forage cactus hay, at a passage rate of 5%/h, showed the highest ED for DM (60.5%, CP (81.1%, NDF (21.6%, ADF (27.9% and HEM (58.0%.

  19. Atividade microbiana do solo em pomar de laranja em resposta ao cultivo de adubos verdes Soil microbialactivities in orchard citrus as a response to green manure cultivation

    Directory of Open Access Journals (Sweden)

    Thais Nucci Buzinaro

    2009-06-01

    Full Text Available Adubos verdes (AVs são condicionadores do solo utilizados para a melhoria da estrutura e fertilidade do solo. Este estudo avaliou o efeito de Crotalaria spectabilis Roth (crotalária, Cajanus cajan (L Millsp (guandu e Brachiaria decumbens Stapf (braquiária nas propriedades microbiológicas e bioquímicas do solo sob laranjal. As leguminosas foram plantadas nas entrelinhas do laranjal e, após 3 meses, cortadas e lançadas nas entrelinhas. A braquiária, já estabelecida, foi roçada e lançada nas entrelinhas. Após 5 meses, as amostras de solo foram coletadas na entrelinha e na linha, na profundidade de 0-20 cm. O delineamento experimental foi em parcela subdividida. Efeito significativo (pGreen manure (GM is a soil conditioner used to improve soil structure and fertility. This study evaluated the effect of Crotalaria spectabilis Roth (showy rattlebox, Cajanus cajan (L Millsp (pigeonpea and Brachiaria decumbens Stapf (brachiaria on soil microbial and biochemical attributes of the citrus orchard. The leguminous was planted at the intercrops and, after 3 months, they were cut and applied in the intercrops. The grown brachiaria was cut and applied in the intercrops. After 5 months, the soil samples were taken from the rows and intercrops in the depth of 0-20 cm. A split-plot design was used. Significant effect (p < 0.05% from the GM application on the bacteria counts and dehydrogenase and nitrifying activities was obtained an it increased, on average, 20, 39, and 190%, respectively. Fungi number decreased 57%. Urease and solubilizing activities and organic matter and moisture contents were not influenced by the GM application. Among the plants, significant responses were found due to the stimulating activities of dehydrogenase of 57% by pigeonpea, urease 58% by brachiaria, solubilizing 346% by showy rattlebox and nitrifying 236% by pigeonpea or brachiaria in relation to the others GM. These results suggest that GM application for successive

  20. Composição química e contribuição nutritiva de cecotrofos de diferentes dietas Chemical composition and nutritive contribution of cecothrophes from different diets

    Directory of Open Access Journals (Sweden)

    Augusto Vidal da Costa Gomes

    1999-01-01

    Full Text Available Os efeitos de cinco fontes de fibra sobre a produção, composição química e a contribuição nutritiva dos cecotrofos em termos de matéria seca e proteína bruta foram estudados. Quarenta coelhas da raça Nova Zelândia branco foram alojadas em gaiolas metabólicas, por 12 dias, para adaptação às dietas. No 13º dia, para evitar a coprofagia, os animais receberam um colar de madeira por um período de 24 horas, procedendo-se à coleta dos cecotrofos de duas em duas horas. A dieta padrão tinha como principal fonte de fibra o feno de alfafa. As outras dietas foram caracterizadas pela substituição isométrica do feno de alfafa por feno de guandu (Cajanus cajan, palha de feijão, palha e sabugo de milho branco e o feno coast-cross (Cynodon dactylon. Foi observado efeito do tipo de dieta sobre a composição química dos cecotrofos e o teor de PB ingerida por cecotrofia, porém a produção de cecotrofos não diferiu entre dietas.The effects of five fiber sources on the production, chemical composition and cecotrophes nutritive contribution on dry matter and crude protein base were studied. Forty White New Zealand female rabbits were housed in metabolic cage for 12 days to adaptation to the diets. In the 13th day, to prevent coprophagy, the animals received a necklace of wood for a period of 24 hours, and the cecotrophes were collected every two hours. The standard diet had the alfalfa hay as a mainly fiber source. The other diets were characterized by isometric replacement of alfalfa hay by pigeon pea hay (Cajanus cajan, bean straw, white corn with cob and husks and coast-cross hay (Cynodon dactylon. Effect of diet type on cecotrophes chemical composition and crude protein content ingested by cecotrophy was observed, however the cecotrophes production was not different among diets.

  1. Tree Species Selection Trial in Mountain Regions with Karst Feature of Yunnan Southeast%滇东南岩溶山区退耕还林适宜造林树种筛选试验

    Institute of Scientific and Technical Information of China (English)

    李军; 李卓新; 谷勇

    2006-01-01

    地处红河流域中段的滇东岩溶山区退耕还林区适宜造林树种的筛选研究,对发展当地经济、保护岩溶生态环境结果具有重大意义.研究表明:印楝(Azadirachta indica)、甜酸角(Tamarindus indica)、墨西哥柏(Cupressus lusitanica)、马鹿花(Puer ariawallichii)、木豆(Cajanus cajan)等树种适宜在海拔500~800 m地带造林;思茅松(Pinus kesiya var.langbianensis)、墨西哥柏、车桑子(Dodonaea viscose)、马鹿花、木豆等树种适宜在海拔1 000~1 500 m的地带造林,冬樱花(Cerasus cerasoides)、圆柏(Sabina chinensis)、板栗(Castanea mdlissima)、杉木(Cunninghamia lanceolata)等树种适宜在海拔1 800~2 000 m的地带造林.冬樱桃、思茅松、墨西哥柏、印楝等采用百日苗容器苗造林,杉木、圆柏、板栗、甜酸角等采用1年生容器大苗造林效果更好.

  2. Improving agricultural productivity in the savannah of Tabasco State, Mexico: II. Management of nitrogen-fixing legumes

    International Nuclear Information System (INIS)

    A series of experiments was carried out in a strongly acidic (with high Al and Fe) and infertile Ultisol to enhance the N inputs derived from biological nitrogen fixation (BNF) through the legume- Rhizobium symbiosis and to draw recommendations on the best agronomic practices needed to intensify sustainable maize production of the subtropical savannahs of Mexico. A large diversity of native Rhizobium strains with variable N fixation capacity exists for use with legume species of agricultural interest in the savannah of Tabasco. Of the black common bean genotypes evaluated, 'N. Sahuatoba, DOR 448, TPL 18 and TPL 16' showed the best potential in terms of BNF (% NdfA and fixed N) and they can be recommended for the savannah of Tabasco. Cajanus cajan and Canavalia ensiformis are the multipurpose legumes with greatest potential in terms of BNF and biomass yield, specially when P fertilized, for the acid Ultisols of the Tabasco region. These legumes can be grown in monoculture and in association with maize and they can be recommended as green manures to reduce the need for chemical N fertilizers. The growth, biomass production and recovery of fertilizer N by selected maize genotypes were better when grown as monoculture than in association with legumes. The wild type 'Dosmesano' was superior to the other maize genotypes tested. Further studies are needed to increase the N inputs from the Rhizobium-legume symbiosis to contribute to improving productivity and sustainability of the agricultural production on the acid savannah soils of Mexico. (author)

  3. Molecular evolution of the HD-ZIP I gene family in legume genomes.

    Science.gov (United States)

    Li, Zhen; Jiang, Haiyang; Zhou, Lingyan; Deng, Lin; Lin, Yongxiang; Peng, Xiaojian; Yan, Hanwei; Cheng, Beijiu

    2014-01-01

    Homeodomain leucine zipper I (HD-ZIP I) genes were used to increase the plasticity of plants by mediating external signals and regulating growth in response to environmental conditions. The way genomic histories drove the evolution of the HD-ZIP I family in legume species was described; HD-ZIP I genes were searched in Lotus japonicus, Medicago truncatula, Cajanus cajan and Phaseolus vulgaris, and then divided into five clades through phylogenetic analysis. Microsynteny analysis was made based on genomic segments containing the HD-ZIP I genes. Some pairs turned out to conform with syntenic genome regions, while others corresponded to those that were inverted, expanded, or contracted after the divergence of legumes. Besides, we dated their duplications by Ks analysis and demonstrated that all the blocks were formed after the monocot-dicot split; we observed Ka/Ks ratios representing strong purifying selections in the four legume species which might have been followed by gene loss and rearrangement. PMID:24095777

  4. Two rhizobacterial strains, individually and in interactions with Rhizobium sp., enhance fusarial wilt control, growth, and yield in pigeon pea.

    Science.gov (United States)

    Dutta, Swarnalee; Morang, Pranjal; Kumar S, Nishanth; Dileep Kumar, B S

    2014-09-01

    A Pseudomonas aeruginosa strain, RRLJ 04, and a Bacillus cereus strain, BS 03, were tested both individually and in combination with a Rhizobium strain, RH 2, for their ability to enhance plant growth and nodulation in pigeon pea (Cajanus cajan L.) under gnotobiotic, greenhouse and field conditions. Both of the rhizobacterial strains exhibited a positive effect on growth in terms of shoot height, root length, fresh and dry weight, nodulation and yield over the non-treated control. Co-inoculation of seeds with these strains and Rhizobium RH 2 also reduced the number of wilted plants, when grown in soil infested with Fusarium udum. Gnotobiotic studies confirmed that the suppression of wilt disease was due to the presence of the respective PGPR strains. Seed bacterization with drug-marked mutants of RRLJ 04 and BS 03 confirmed their ability to colonize and multiply along the roots. The results suggest that co-inoculation of these strains with Rhizobium strain RH 2 can be further exploited for enhanced growth, nodulation and yield in addition to control of fusarial wilt in pigeon pea. PMID:25224506

  5. Siderophore as a potential plant growth-promoting agent produced by Pseudomonas aeruginosa JAS-25.

    Science.gov (United States)

    Sulochana, M B; Jayachandra, S Y; Kumar, S Anil; Parameshwar, A B; Reddy, K Mohan; Dayanand, A

    2014-09-01

    Siderophores scavenges Fe(+3) from the vicinity of the roots of plants, and thus limit the amount of iron required for the growth of pathogens such as Fusarium oxysporum, Pythium ultimum, and Fusarium udum, which cause wilt and root rot disease in crops. The ability of Pseudomonas to grow and to produce siderophore depends upon the iron content, pH, and temperature. Maximum yield of siderophore of 130 μM was observed at pH 7.0 ± 0.2 and temperature of 30 °C at 30 h. The threshold level of iron was 50 μM, which increases up to 150 μM, favoring growth but drastically affecting the production of siderophore by Pseudomonas aeruginosa JAS-25. The seeds of agricultural crops like Cicer arietinum (chick pea), Cajanus cajan (pigeon pea), and Arachis hypogaea (ground nut) were treated with P. aeruginosa JAS-25, which enhanced the seed germination, root length, shoot length, and dry weight of chick pea, pigeon pea, and ground nut plants under pot studies. The efficient growth of the plants was not only due to the biocontrol activity of the siderophore produced by P. aeruginosa JAS-25 but also may be by the production of indole acetic acid (IAA), which influences the growth of the plants as phytohormones. PMID:25062779

  6. Study on Plant Species Composition for Revegetatation at Zinc and Indium Mining Area%锌铟矿区植被结构优化造林模式的研究

    Institute of Scientific and Technical Information of China (English)

    常恩福; 侬时增; 刘永国; 李娅; 钱兴廷; 安康发; 周文华

    2013-01-01

    Silvicultural experiments including 10 species composition of 12 tested plant species were conducted in Zinc and indium mine waste accumulation site located in Dulong of Maguan county of Yunnan province from 2010 to 2013.Twelve tested plant species were Cupressus lusitanica,Alnus nepalensis,A.cremastogyne cv.yanshan,Ficus microcarpa vat.pusillifolia,Celtis kunmingensis,Pinus eltiottii,Buxus sinica,Ligustrum quihoui,Cajanus cajan,Neyraudia reynaudiana,Festuca arundinacea and Lolium perenne.Three years after field planting,the growth increments of 10 designed species composition were measured,and the soil nutritional status were analyzed as well.By considering the ecological status,site characteristics and the objectives of revegetation,four plant species with promising adaptability,afforestation survival rate and preserving rate were screened out,they were A.cremastogyne cv.yanshan,Cajanus cajan,A.nepalensis and Neyraudia reynaudiana.Combining A.cremastogyne cv.yanshan with Neyraudia reynaudiana or Cajanus cajan with Neyraudia reynaudiana,could speed up the positive succession,and form multilayer stand structure,with the survival rate over 95 % and preserving rate over 87 %.The average tree height and DBH of three years A.cremastogyne cv.yanshan were 4.79 m and 4.5 cm respectively,the average tree height and DBH of three years Cajanus cajan were 2.16 m and 2.8 cm respectively,the average height of Neyraudia reynaudiana was 2.18 m.The experimental results also indicated that at the initial stage of revegetation,choosing proper plant species and combining the species scientifically,the biodiversity could be maintained effectively,and the ideal community structure could be achieved,which therefore quite promising for the improve the soil fertility of the site.%2010 ~2013年在马关都龙曼家寨锌铟矿山采用墨西哥柏、旱冬瓜、川滇桤木、小叶榕、滇朴、湿地松、黄杨、小叶女桢、木豆、类芦、苇状羊茅、黑麦草12

  7. Allelopathic effects of Leucaena leucocephala leaf litter on some forest and agricultural crops grown in nursery

    Institute of Scientific and Technical Information of China (English)

    Romel Ahmed; A. T. M. Rafiqul Hoque; Mohammed Kamal Hossain

    2008-01-01

    An experiment was conducted to assess the effect of leaf litter of Leucaena leucocephala on two forest crops Sada koroi (Albizia procera),Ipil ipil (L.leucocephala) and three agricultural crops Falen (Vigna unguiculata),Chickpea (Cicer arietinum) and Arhor (Cajanus cajan) in the nursery of the Institute of Forestry and Environmental Sciences,Chittagong University,Bangladesh,in a Randomized Block Design.Results suggested that leaf litters of L.leucocephala induced inhibitory effects on germination and growth of bioassay.It was also found that the effect depended on concentration of extract and litterfall,type of receptor species.Higher concentration of the materials had the higher effect and vice versa.Growth response of receptor crops varied with the variation of leaf litter application.The study revealed that application of low-dose leaf litter specially litter of 10 g(m-2 had stimulating effect on shoot growth of C.arietinum,V.unguiculata and A.procera.While in all other cases significant inhibitory effect was observed and it was significantly increased with the increase of leaf litter application.However,the trend of inhibition was uneven with treatments.Root growth was found to be more affected than shoot growth.

  8. LegumeIP 2.0--a platform for the study of gene function and genome evolution in legumes.

    Science.gov (United States)

    Li, Jun; Dai, Xinbin; Zhuang, Zhaohong; Zhao, Patrick X

    2016-01-01

    The LegumeIP 2.0 database hosts large-scale genomics and transcriptomics data and provides integrative bioinformatics tools for the study of gene function and evolution in legumes. Our recent updates in LegumeIP 2.0 include gene and protein sequences, gene models and annotations, syntenic regions, protein families and phylogenetic trees for six legume species: Medicago truncatula, Glycine max (soybean), Lotus japonicus, Phaseolus vulgaris (common bean), Cicer arietinum (chickpea) and Cajanus cajan (pigeon pea) and two outgroup reference species: Arabidopsis thaliana and Poplar trichocarpa. Moreover, the LegumeIP 2.0 features the following new data resources and bioinformatics tools: (i) an integrative gene expression atlas for four model legumes that include 550 array hybridizations from M. truncatula, 962 gene expression profiles of G. max, 276 array hybridizations from L. japonicas and 56 RNA-Seq-based gene expression profiles for C. arietinum. These datasets were manually curated and hierarchically organized based on Experimental Ontology and Plant Ontology so that users can browse, search, and retrieve data for their selected experiments. (ii) New functions/analytical tools to query, mine and visualize large-scale gene sequences, annotations and transcriptome profiles. Users may select a subset of expression experiments and visualize and compare expression profiles for multiple genes. The LegumeIP 2.0 database is freely available to the public at http://plantgrn.noble.org/LegumeIP/. PMID:26578557

  9. Evaluation of N2—Fixing Capacites of Herbaceous and Shrub Legumes Under Field Condition

    Institute of Scientific and Technical Information of China (English)

    XJINGGUANGXI; SHISHULIAN; 等

    1998-01-01

    Natural 15N abundance method was used in this study to investigate the N2-fiing capacities of several herbaceous and shrub legumes by a field experiment,For herbaceous legumes,the results were in consistent with those obtained by a pot experiment .Crotalaria mucronata Desv.had a higher,N2-fixing capacity than Vigna sinensis(L.)Savi.For shurub legumes,N2-fixing Capacity under field condition was slightly different from that in pot experiment.These results demonstrated that the natural 15N abundance method was applicable to evaluating N2-fixing capacities of herbaceous and shrub legumes,and that pot experiment was suitable for evaluating the N2-fixing capacities of not only herbaceous legumes but also shrub legumes. Leguminous N2-fixing plants differed in response to phosphorus fertilization,Phosphorus fertilizer appication greatly increased the percentage of nitrogen derived from air (% N dfa)and total amount of N2 fixed by Lespedez formosa(Vog.)Koehne (Jiangxi),Phosphorus fertilizer showed no signifcant effect on the N2-fixing percentage of Cajanus cajan(L.)Millsp.but increased its total biomass,thus increasing the total amount of N2 fixed.

  10. Integrated consensus map of cultivated peanut and wild relatives reveals structures of the A and B genomes of Arachis and divergence of the legume genomes.

    Science.gov (United States)

    Shirasawa, Kenta; Bertioli, David J; Varshney, Rajeev K; Moretzsohn, Marcio C; Leal-Bertioli, Soraya C M; Thudi, Mahendar; Pandey, Manish K; Rami, Jean-Francois; Foncéka, Daniel; Gowda, Makanahally V C; Qin, Hongde; Guo, Baozhu; Hong, Yanbin; Liang, Xuanqiang; Hirakawa, Hideki; Tabata, Satoshi; Isobe, Sachiko

    2013-04-01

    The complex, tetraploid genome structure of peanut (Arachis hypogaea) has obstructed advances in genetics and genomics in the species. The aim of this study is to understand the genome structure of Arachis by developing a high-density integrated consensus map. Three recombinant inbred line populations derived from crosses between the A genome diploid species, Arachis duranensis and Arachis stenosperma; the B genome diploid species, Arachis ipaënsis and Arachis magna; and between the AB genome tetraploids, A. hypogaea and an artificial amphidiploid (A. ipaënsis × A. duranensis)(4×), were used to construct genetic linkage maps: 10 linkage groups (LGs) of 544 cM with 597 loci for the A genome; 10 LGs of 461 cM with 798 loci for the B genome; and 20 LGs of 1442 cM with 1469 loci for the AB genome. The resultant maps plus 13 published maps were integrated into a consensus map covering 2651 cM with 3693 marker loci which was anchored to 20 consensus LGs corresponding to the A and B genomes. The comparative genomics with genome sequences of Cajanus cajan, Glycine max, Lotus japonicus, and Medicago truncatula revealed that the Arachis genome has segmented synteny relationship to the other legumes. The comparative maps in legumes, integrated tetraploid consensus maps, and genome-specific diploid maps will increase the genetic and genomic understanding of Arachis and should facilitate molecular breeding. PMID:23315685

  11. Sinorhizobium fredii HH103 bacteroids are not terminally differentiated and show altered O-antigen in nodules of the Inverted Repeat-Lacking Clade legume Glycyrrhiza uralensis.

    Science.gov (United States)

    Crespo-Rivas, Juan C; Guefrachi, Ibtissem; Mok, Kenny C; Villaécija-Aguilar, José A; Acosta-Jurado, Sebastián; Pierre, Olivier; Ruiz-Sainz, José E; Taga, Michiko E; Mergaert, Peter; Vinardell, José M

    2016-09-01

    In rhizobial species that nodulate inverted repeat-lacking clade (IRLC) legumes, such as the interaction between Sinorhizobium meliloti and Medicago, bacteroid differentiation is driven by an endoreduplication event that is induced by host nodule-specific cysteine rich (NCR) antimicrobial peptides and requires the participation of the bacterial protein BacA. We have studied bacteroid differentiation of Sinorhizobium fredii HH103 in three host plants: Glycine max, Cajanus cajan and the IRLC legume Glycyrrhiza uralensis. Flow cytometry, microscopy analyses and viability studies of bacteroids as well as confocal microscopy studies carried out in nodules showed that S. fredii HH103 bacteroids, regardless of the host plant, had deoxyribonucleic acid (DNA) contents, cellular sizes and survival rates similar to those of free-living bacteria. Contrary to S. meliloti, S. fredii HH103 showed little or no sensitivity to Medicago NCR247 and NCR335 peptides. Inactivation of S. fredii HH103 bacA neither affected symbiosis with Glycyrrhiza nor increased bacterial sensitivity to Medicago NCRs. Finally, HH103 bacteroids isolated from Glycyrrhiza, but not those isolated from Cajanus or Glycine, showed an altered lipopolysaccharide. Our studies indicate that, in contrast to the S. meliloti-Medicago model symbiosis, bacteroids in the S. fredii HH103-Glycyrrhiza symbiosis do not undergo NCR-induced and bacA-dependent terminal differentiation. PMID:26521863

  12. 木豆栽培技术

    Institute of Scientific and Technical Information of China (English)

    肖国滨; 廖绵清; 韩仁道; 刘小三; 程飞虎

    2002-01-01

    @@ 木豆[Cajanus cajan(L.)Millspaugh]原产于热带和亚热带,俗称大豆树,又有鸽豆、蓉豆、柳豆之称.木豆除具有豆类作物的一般性状外,还具有强大的根系、繁茂的枝条、美观的树形和鲜艳的花序四大优良特性.木豆可以作粮用、蔬菜用、饲料用、药用和绿肥用,还可用作水土保持和美化环境,薪材、建材及工业原料(纸浆原料)和生产紫胶等,在农林复合生态系统中的利用潜力巨大,在南方红壤丘陵地区推广应用前景十分广阔.现将木豆栽培技术简要介绍如下:

  13. Deep sequencing of pigeonpea sterility mosaic virus discloses five RNA segments related to emaraviruses.

    Science.gov (United States)

    Elbeaino, Toufic; Digiaro, Michele; Uppala, Mangala; Sudini, Harikishan

    2014-08-01

    The sequences of five viral RNA segments of pigeonpea sterility mosaic virus (PPSMV), the agent of sterility mosaic disease (SMD) of pigeonpea (Cajanus cajan, Fabaceae), were determined using the deep sequencing technology. Each of the five RNAs encodes a single protein on the negative-sense strand with an open reading frame (ORF) of 6885, 1947, 927, 1086, and 1,422 nts, respectively. In order, from RNA1 to RNA5, these ORFs encode the RNA-dependent RNA polymerase (p1, 267.9 kDa), a putative glycoprotein precursor (p2, 74.3 kDa), a putative nucleocapsid protein (p3, 34.6 kDa), a putative movement protein (p4, 40.8 kDa), while p5 (55 kDa) has an unknown function. All RNA segments of PPSMV showed the highest identity with orthologs of fig mosaic virus (FMV) and Rose rosette virus (RRV). In phylogenetic trees constructed with the amino acid sequences of p1, p2 and p3, PPSMV clustered consistently with other emaraviruses, close to clades comprising members of other genera of the family Bunyaviridae. Based on the molecular characteristics unveiled in this study and the morphological and epidemiological features similar to other emaraviruses, PPSMV seems to be the seventh species to join the list of emaraviruses known to date and accordingly, its classification in the genus Emaravirus seems now legitimate. PMID:24685674

  14. Construction and pilot screening of a signature-tagged mutant library of Sinorhizobium fredii.

    Science.gov (United States)

    Wang, Dan; Wang, Yuan Chun; Wu, Li Juan; Liu, Jian Xin; Zhang, Pan; Jiao, Jian; Yan, Hui; Liu, Tao; Tian, Chang Fu; Chen, Wen Xin

    2016-03-01

    Sinorhizobium fredii is well known for its ability to establish symbiosis with diverse legumes such as Glycine max (soybean, determinate nodules) and Cajanus cajan (pigeon pea, indeterminate nodules). In order to make screening of S. fredii genes related to symbiosis cost-effective, we constructed a large Tn5 insertion mutant library of S. fredii CCBAU45436 using the signature-tagged mutagenesis (STM) technique. This STM library contains a total of 25,500 independent mutants distributed in 17 sublibraries tagged by corresponding distinct DNA bar-code sequences. After the pilot screening of 255 mutants in 15 batches, Tag85-4, Tag4-17, Tag4-11 and Tag10-13 were found to have attenuated competitiveness (0-30 % in nodule occupation) compared to the wild-type strain when inoculated on soybean. Further characterization of these mutants suggests that Tag4-11 (a pyrC mutant) and Tag10-13 (a nrdJ mutant) are defective in establishing symbiosis with soybean. The pyrC mutant induced uninfected pseudonodules while the nrdJ mutant formed significantly more nodules containing bacteroids with poor persistence ability. When these two mutants were tested on pigeon pea, host-specific symbiotic defects were found. These results demonstrated the STM library as a valuable resource for identifying S. fredii genes relevant to symbiosis. PMID:26472206

  15. Study the effect of insecticide dimethoate on photosynthetic pigments and photosynthetic activity of pigeon pea: Laser-induced chlorophyll fluorescence spectroscopy.

    Science.gov (United States)

    Pandey, Jitendra Kumar; Dubey, Gunjan; Gopal, R

    2015-10-01

    Pigeon pea is one of the most important legume crops in India and dimethoate is a widely used insecticide in various crop plants. We studied the effect of dimethoate on growth and photosynthetic activity of pigeon pea plants over a short and long term exposure. Plant growth parameters, photosynthetic pigment content and chlorophyll fluorescence response of pigeon pea (Cajanus cajan L.) plants treated with various concentrations of the insecticide dimethoate (10, 20, 40 and 80 ppm) have been compared for 30 days at regular intervals of 10 days each. Laser induced chlorophyll fluorescence spectra and fluorescence-induction kinetics (FIK) curve of dimethoate treated pigeon pea plants were recorded after 10, 20 and 30 days of treatment. Fluorescence intensity ratio at the two fluorescence maxima (F685/F730) was calculated by evaluating curve-fitted parameters. The variable chlorophyll fluorescence decrease ratio (Rfd) was determined from the FIK curves. Our study revealed that after 10 days of treatment, 10 ppm of dimethoate showed stimulatory response whereas 20, 40 and 80 ppm of dimethoate showed inhibitory response for growth and photosynthetic activity of pigeon pea plants, but after 20 and 30 days of treatment all the tested concentrations of dimethoate became inhibitory. This study clearly shows that dimethoate is highly toxic to the pigeon pea plant, even at very low concentration (10 ppm), if used for a prolonged duration. Our study may thus be helpful in determining the optimal dose of dimethoate in agricultural practices. PMID:25228224

  16. Biofortification of mungbean (Vigna radiata) as a whole food to enhance human health.

    Science.gov (United States)

    Nair, Ramakrishnan M; Yang, Ray-Yu; Easdown, Warwick J; Thavarajah, Dil; Thavarajah, Pushparajah; Hughes, Jacqueline d'A; Keatinge, J D H Dyno

    2013-06-01

    Mungbean (Vigna radiata (L.) R. Wilczek var. radiata) is one of the most important pulse crops grown in South, East and Southeast Asia. It provides significant amounts of protein (240 g kg(-1)) and carbohydrate (630 g kg(-1)) and a range of micronutrients in diets. Mungbean protein and carbohydrate are easily digestible and create less flatulence than proteins derived from other legumes. In addition, mungbean is lower in phytic acid (72% of total phosphorus content) than pigeonpea (Cajanus cajan L. Millsp.), soybean (Glycine max L.) and cereals; phytic acid is commonly found in cereal and legume crops and has a negative impact on iron and zinc bioavailability in plant-based diets. Owing to its palatable taste and nutritional quality, mungbean has been used as an iron-rich whole food source for baby food. The wide genetic variability of mineral concentrations (e.g. 0.03-0.06 g Fe kg(-1), 0.02-0.04 g Zn kg(-1)) in mungbean indicates possibilities to improve its micronutrient content through biofortification. Therefore biofortification of existing mungbean varieties has great potential for enhancing the nutritional quality of diets in South and Southeast Asia, where protein and micronutrient malnutrition are among the highest in the world. This review paper discusses the importance of mungbean in agricultural production and traditional diets and the potential of enhancing the nutritional quality of mungbean through breeding and other means, including agronomic practices. PMID:23426879

  17. Pigeonpea genotypes influence parasitization preference and survival and development of the Helicoverpa armigera larval parasitoid, Campoletis chlorideae.

    Science.gov (United States)

    Hugar, Shiddalingappa V; Sharma, Hari C; Basavan Goud, Kondikallu

    2014-01-01

    Studies were undertaken to identify pigeonpea, Cajanus cajan (L.) Millspaugh and the wild relative of pigeonpea, Cajanus scarabaeoides (L.) (accession ICPW 125,) genotypes that are hospitable to the pod borer, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) larval parasitoid, Campoletis chlorideae Uchida (Hymenoptera: Ichneumonidae) for the management of this pest in pigeonpea based cropping systems. Percentage parasitization of the H. armigera larvae by the C. chlorideae females was greater under no-choice conditions than under multi-choice conditions because of forced parasitization under no-choice conditions. Lowest parasitization was recorded on the wild relative, ICPW 125, which may be due to long nonglandular hairs and low survival of H. armigera larvae. Parasitization of H. armigera larvae was greater under no-choice, dual-choice and/or multi-choice conditions on ICPL 87, ICPL 87119 and ICPL 87091, which are susceptible to H. armigera, than on the pod borer-resistant genotypes ICPL 332WR, ICPL 84060 and ICPB 2042; while survival and development of the parasitoid was better on H. armigera larvae fed on ICPL 87, ICPL 87119, LRG 41, ICP 7035 and ICPL 87091 than on ICPL 332WR, ICPL 84060, ICPB 2042 and ICPW 125. The genotypes ICPL 87, ICPL 87119, LRG 42 and ICPL 87091 that are hospitable to C. chloridae, are better suited for use in integrated pest management to minimize the losses due to H. armigera in pigeonpea. PMID:25110629

  18. Productive performance of naked neck chickens that were fed leaf meal shrubs

    Directory of Open Access Journals (Sweden)

    Santos M Herrera G.

    2015-12-01

    Full Text Available Objective. To compare the productive performance of naked neck chickens (phases of initiation, growth and final that were fed meals Gliricidia sepium, Cajanus cajan and Morus alba leaves. Materials and methods. 192 chickens, 1-84 days of age were distributed in a randomized block design with three experimental groups (5% of shrub in the diet, 48 animals/ group, eight replicates/ treatment, six animals/ reply and three animals/ sex in each replicate were used. The control group consumed diet based on corn and soybeans. They were reared on floor. Weighed every seven days. Weight gain, voluntary intake, conversion, balance and efficiency of feed utilization were calculated. Results. The highest total feed intake and average daily gain in rearing were 37.43g 9509.96 g respectively for M. alba (p<0.05, which also presented the best efficiency of energy and protein. Meanwhile, G. sepium showed the lowest values. Conclusions. It is possible to replace 5% of corn and soy in the diet of naked neck chickens, with the inclusion of leaf meal M. alba and get a favorable productive behavior.

  19. Performance evaluation of natural draft based agricultural residues charcoal system

    Energy Technology Data Exchange (ETDEWEB)

    Patil, K.N.; Ramana, P.V.; Singh, R.N. [Sardar Patel Renewable Energy Research Inst., Gujarat (India)

    2000-07-01

    A natural draft based agricultural residues charcoal reactor has been described herein along with its performance details. Instead of releasing pyrogases into the atmosphere, these gases are burnt inside the charcoal reactor, offering better energy efficiency and environmental acceptability. Agricultural residues like arhar stalks (Cajanus cajan), saw mill woody waste, Ipomoea (Ipomoea fistulasa, Syn. Ipomoea Carnea) and babul wood (Acacia nilotica), all sun dried, were used as the feedstocks for charcoal making. Saleable charcoal (SC) yield was in the range of 28 to 47% dry basis (db) with the maximum from saw mill woody waste and the minimum from Ipomoea. Fixed carbon (FC) content in the SC varies from 69 to 77% (db) in the agricultural residues based charcoal. Babul wood charring gave the highest SC yield (50%, db) and the best quality charcoal in terms of FC (80%, db). Economic analysis revealed that if the system developed was operated annually for 4000 h, the user could have a net profit of around Rs.l,00,000/-(US $2500). (author)

  20. Late Embryogenesis Abundant (LEA) proteins in legumes.

    Science.gov (United States)

    Battaglia, Marina; Covarrubias, Alejandra A

    2013-01-01

    Plants are exposed to different external conditions that affect growth, development, and productivity. Water deficit is one of these adverse conditions caused by drought, salinity, and extreme temperatures. Plants have developed different responses to prevent, ameliorate or repair the damage inflicted by these stressful environments. One of these responses is the activation of a set of genes encoding a group of hydrophilic proteins that typically accumulate to high levels during seed dehydration, at the last stage of embryogenesis, hence named Late Embryogenesis Abundant (LEA) proteins. LEA proteins also accumulate in response to water limitation in vegetative tissues, and have been classified in seven groups based on their amino acid sequence similarity and on the presence of distinctive conserved motifs. These proteins are widely distributed in the plant kingdom, from ferns to angiosperms, suggesting a relevant role in the plant response to this unfavorable environmental condition. In this review, we analyzed the LEA proteins from those legumes whose complete genomes have been sequenced such as Phaseolus vulgaris, Glycine max, Medicago truncatula, Lotus japonicus, Cajanus cajan, and Cicer arietinum. Considering their distinctive motifs, LEA proteins from the different groups were identified, and their sequence analysis allowed the recognition of novel legume specific motifs. Moreover, we compile their transcript accumulation patterns based on publicly available data. In spite of the limited information on these proteins in legumes, the analysis and data compiled here confirm the high correlation between their accumulation and water deficit, reinforcing their functional relevance under this detrimental conditions. PMID:23805145

  1. Genetic diversity and demographic history of Cajanus spp. illustrated from genome-wide SNPs.

    Science.gov (United States)

    Saxena, Rachit K; von Wettberg, Eric; Upadhyaya, Hari D; Sanchez, Vanessa; Songok, Serah; Saxena, Kulbhushan; Kimurto, Paul; Varshney, Rajeev K

    2014-01-01

    Understanding genetic structure of Cajanus spp. is essential for achieving genetic improvement by quantitative trait loci (QTL) mapping or association studies and use of selected markers through genomic assisted breeding and genomic selection. After developing a comprehensive set of 1,616 single nucleotide polymorphism (SNPs) and their conversion into cost effective KASPar assays for pigeonpea (Cajanus cajan), we studied levels of genetic variability both within and between diverse set of Cajanus lines including 56 breeding lines, 21 landraces and 107 accessions from 18 wild species. These results revealed a high frequency of polymorphic SNPs and relatively high level of cross-species transferability. Indeed, 75.8% of successful SNP assays revealed polymorphism, and more than 95% of these assays could be successfully transferred to related wild species. To show regional patterns of variation, we used STRUCTURE and Analysis of Molecular Variance (AMOVA) to partition variance among hierarchical sets of landraces and wild species at either the continental scale or within India. STRUCTURE separated most of the domesticated germplasm from wild ecotypes, and separates Australian and Asian wild species as has been found previously. Among Indian regions and states within regions, we found 36% of the variation between regions, and 64% within landraces or wilds within states. The highest level of polymorphism in wild relatives and landraces was found in Madhya Pradesh and Andhra Pradesh provinces of India representing the centre of origin and domestication of pigeonpea respectively. PMID:24533111

  2. Propriedades do solo e sistema radicular do feijoeiro irrigado em rotação de culturas

    Directory of Open Access Journals (Sweden)

    E. B. Wutke

    2000-09-01

    Full Text Available O crescimento do sistema radicular do feijoeiro irrigado, cultivar IAC-Carioca, sob pivô central, em rotação com pousio, milho, aveia preta, Crotalaria juncea L., guandu (Cajanus cajan (L. Millsp. e mucuna preta (Mucuna aterrima, no período não-convencional de cultivo no outono-inverno, foi avaliado de 1993 a 1995, em Latossolo Vermelho eutroférrico típico, em área experimental do Instituto Agronômico, em Ribeirão Preto, São Paulo. A rotação de culturas do feijoeiro com milho e adubos verdes favoreceu a redução da resistência do solo à penetração na camada arável, garantiu a manutenção do teor de matéria orgânica do solo, bem como possibilitou a redução da acidez e o aumento do índice de saturação por bases (V% em profundidade em relação ao teor inicial. A velocidade de infiltração básica da água no solo foi favorecida pela inclusão da mucuna preta, da Crotalaria juncea L. e do milho, no esquema de rotações. A profundidade efetiva observada do seu sistema radicular foi de 0,35 a 0,40 m.

  3. OS SISTEMAS AGROFLORESTAIS COMO ALTERNATIVA ECONÔMICA EM PEQUENAS PROPRIEDADES RURAIS: ESTUDO DE CASO

    Directory of Open Access Journals (Sweden)

    Mário Jorge Campos dos Santos

    2002-05-01

    Full Text Available Os Sistemas Agroflorestais (SAFs constituem-se em uma alternativa de uso da terra para aliar a estabilidade do ecossistema visando à eficiência e otimização de recursos naturais na produção de forma integrada e sustentada. O objetivo deste estudo de caso foi avaliar a viabilidade econômica de um sistema agroflorestal localizado na região do Pontal do Paranapanema. O SAF em estudo é formada por espécies agrícolas: milho (Zea maiz, feijão guandu (Cajanus cajan, carioquinha, (Vigna unguiculata, preto (Phaseolus vulgaris e mandioca (Manihot esculenta com espécies florestais: Eucalyptus citriodora e Eucalyptus camaldulensis. A rentabilidade econômica do sistema foi mensurada, utilizando Valor Presente Líquido (VPL, Razão Benefício/Custo (RB/C para as culturas agrícolas anuais e Valor Esperado da terra (VET para os componentes madeireiros. Para tais cálculos foi escolhida uma taxa de desconto de 6%. O estudo demonstrou que o sistema agroflorestal apresentou rentabilidade econômica positiva, e que podem ser adotados por pequenos produtores. Assim, pode-se concluir que os sistemas agroflorestais são economicamente viáveis para pequenos produtores rurais trabalhando nas mesmas condições apresentadas neste estudo.

  4. 贵州亚热带地区木豆推广应用现状及其发展对策

    Institute of Scientific and Technical Information of China (English)

    龚德勇; 左德川

    2005-01-01

    木豆(Cajanus cajan (Linn)miusp)为蝶形花科木豆属多年生常绿直立小灌木,又名树黄豆、鸽豆、柳豆等,是世界第六大食用豆类之一,也是唯一的木本食用豆类嵋。,广泛分布于非洲和亚洲的热带和亚热带地区.2000年开始,贵州省亚热带作物研究所先后从广西农科院引进4个印度改良型木豆品种分别在南、北盘江流域的贞丰、望谟等亚热带低热地区进行试种观察取得成功后,引起了相关地区和部门的极大关注,竟相引种试验示范和推广。2002~2003年,贞丰县发展木豆种植面积1000hm2以上。但是大面积栽培应用中存在区域布局不尽合理、配套栽培技术缺乏、

  5. Soil Organic Matter Quality of an Oxisol Affected by Plant Residues and Crop Sequence under No-Tillage

    Science.gov (United States)

    Cora, Jose; Marcelo, Adolfo

    2013-04-01

    Plant residues are considered the primarily resource for soil organic matter (SOM) formation and the amounts and properties of plant litter are important controlling factors for the SOM quality. We determined the amounts, quality and decomposition rate of plant residues and the effects of summer and winter crop sequences on soil organic C (TOC) content, both particulate organic C (POC) and mineral-associated organic C (MOC) pools and humic substances in a Brazilian Rhodic Eutrudox soil under a no-tillage system. The organic C analysis in specifics pools used in this study was effective and should be adopted in tropical climates to evaluate the soil quality and the sustainability of various cropping systems. Continuous growth of soybean (Glycine max L. Merrill) on summer provided higher contents of soil POC and continuous growth of maize (Zea mays L.) provided higher soil humic acid and MOC contents. Summer soybean-maize rotation provided the higher plant diversity, which likely improved the soil microbial activity and the soil organic C consumption. The winter sunn hemp (Crotalaria juncea L.), pigeon pea (Cajanus cajan (L.) Millsp), oilseed radish (Raphanus sativus L.) and pearl millet (Pennisetum americanum (L.) Leeke) enhanced the soil MOC, a finding that is attributable to the higher N content of the crop residue. Sunn hemp and pigeon pea provided the higher soil POC content. Sunn hemp showed better performance and positive effects on the SOM quality, making it a suitable winter crop choice for tropical conditions with a warm and dry winter.

  6. Cover plants and mineral nitrogen: effects on organic matter fractions in an oxisol under no-tillage in the cerrado

    Directory of Open Access Journals (Sweden)

    Isis Lima dos Santos

    2014-12-01

    Full Text Available Cover plants are essential for the sustainability of no-tillage systems in tropical regions. However, information on the effects of these plants and N fertilization on soil organic matter fractions is still scarce. This study evaluated the effect of cover crops with different chemical composition and of N topdressing on the labile and humified organic matter fractions of an Oxisol of the Cerrado (savanna-like vegetation. The study in a randomized complete block design was arranged in split-plots with three replications. Four cover species were tested in the plots and the presence or absence of N topdressing in the subplot. The following cover species were planted in succession to corn for eight years: Urochloa ruziziensis; Canavalia brasiliensis M. ex Benth; Cajanus cajan (L. Millsp; and Sorghum bicolor (L. Moench. In general, the cultivation of U. ruziziensis increased soil C levels, particularly of C in the humic acid and particulate organic C fractions, which are quality indicators of soil organic matter. The C in humic substances and mineral organic C accounted for the highest proportions of total organic C, demonstrating the strong interaction between organic matter, Fe and Al oxides and kaolinite, which are predominant in these weathered soils of the Cerrado.

  7. Transformation of pWWO in Rhizobium leguminosarum DPT to Engineer Toluene Degrading Ability for Rhizoremediation.

    Science.gov (United States)

    Goel, Garima; Pandey, Piyush; Sood, Anchal; Bisht, Sandeep; Maheshwari, D K; Sharma, G D

    2012-06-01

    Rhizoremediation of organic xenobiotics is based on interactions between plants and their associated micro-organisms. The present work was designed to engineer a bacterial system having toluene degradation ability along with plant growth promoting characteristics for effective rhizoremediation. pWWO harboring the genes responsible for toluene breakdown was isolated from Pseudomonas putida MTCC 979 and successfully transformed in Rhizobium DPT. This resulted in a bacterial strain (DPT(T)) which had the ability to degrade toluene as well as enhance growth of host plant. The frequency of transformation was recorded 5.7 × 10(-6). DPT produced IAA, siderophore, chitinase, HCN, ACC deaminase, solubilized inorganic phosphate, fixed atmospheric nitrogen and inhibited the growth of Fusarium oxysporum and Macrophomina phaseolina in vitro. During pot assay, 50 ppm toluene in soil was found to inhibit the germination of Cajanus cajan seeds. However when the seeds bacterized with toluene degrading P. putida or R. leguminosarum DPT were sown in pots, again no germination was observed. Non-bacterized as well as bacterized seeds germinated successfully in toluene free soil as control. The results forced for an alternative mode of application of bacteria for rhizoremediation purpose. Hence bacterial suspension was mixed with soil having 50 ppm of toluene. Germination index in DPT treated soil was 100% while in P. putida it was 50%. Untreated soil with toluene restricted the seeds to germinate. PMID:23729882

  8. Characterization of the bioactive metabolites from a plant growth-promoting rhizobacteria and their exploitation as antimicrobial and plant growth-promoting agents.

    Science.gov (United States)

    George, Emrin; Kumar, S Nishanth; Jacob, Jubi; Bommasani, Bhaskara; Lankalapalli, Ravi S; Morang, P; Kumar, B S Dileep

    2015-05-01

    A plant growth-promoting bacterial strain, PM 105, isolated from a tea plantation soil from the North Eastern region of India was identified as Pseudomonas aeruginosa through classical and 16S ribosomal DNA (rDNA) gene sequencing. Further studies with this strain confirmed broad spectrum antifungal activity against ten human and plant pathogenic fungal pathogens viz. Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus tubingensis, Candida albicans, Colletotrichum gloeosporioides, Fusarium oxysporum, Pencillium expansum, Rhizoctonia solani, Trichophyton rubrum besides growth-promoting property in cowpea (Vigna unguiculata) and pigeon pea (Cajanus cajan). However, no antibacterial property was exhibited by this strain against the four test bacterial pathogens tested in agar overlay method. The crude bioactive metabolites produced by this strain were isolated with three different solvents that exhibited significant antimicrobial and plant growth-promoting activity. Chloroform extract recorded significant antimicrobial and plant growth-promoting activity. Three major compounds viz. 1-hydroxyphenazine, pyocyanin, and phenazine-1-carboxamide were purified and characterized from crude extracts of this strain by various spectral data. The purified compounds recorded prominent antimicrobial activity but failed to establish the plant growth promotion activity in test crop plants under gnotobiotic conditions. Pyocyanin recorded significant antimicrobial activity, and best activity was recorded against T. rubrum (29 mm), followed by P. expansum (28 mm). These results suggest the use of PM 105 as plant growth-promoting agent in crop plants after successful field trials. PMID:25832181

  9. Consórcio de milho com braquiária e guandu-anão em sistema de dessecação parcial

    Directory of Open Access Journals (Sweden)

    Priscila de Oliveira

    2011-10-01

    Full Text Available O objetivo deste trabalho foi avaliar a viabilidade do consórcio de milho (Zea mays cv. BRS 1035 com braquiária (Urochloa brizantha e guandu-anão (Cajanus cajan, no sistema de dessecação parcial. Os experimentos foram realizados na safra de verão de 2008/2009, em Santo Antônio de Goiás, GO e Ipameri, GO, em blocos completos ao acaso, com cinco repetições. Os tratamentos constituíram-se de cultivos de milho, em consórcio com braquiária e guandu-anão, com a aplicação de nitrogênio mineral em cobertura, à emergência da cultura ou aos 20 dias após esse evento, com ou sem aplicação de subdose de herbicida à braquiária não dessecada. A viabilidade do sistema de dessecação parcial é dependente do manejo da adubação nitrogenada do milho ou do controle do crescimento da braquiária nas faixas não dessecadas.

  10. Os sistemas agroflorestais como alternativa econômica em pequenas propriedades rurais: estudo de caso.

    Directory of Open Access Journals (Sweden)

    Mário Jorge Campos dos Santos

    2010-08-01

    Full Text Available Os Sistemas Agroflorestais (SAFs constituem-se em uma alternativa de uso da terra para aliar a estabilidade do ecossistema visando à eficiência e otimização de recursos naturais na produção de forma integrada e sustentada. O objetivo deste estudo de caso foi avaliar a viabilidade econômica de um sistema agroflorestal localizado na região do Pontal do Paranapanema. O SAF em estudo é formada por espécies agrícolas: milho (Zea maiz, feijão guandu (Cajanus cajan, carioquinha, (Vigna unguiculata, preto (Phaseolus vulgaris e mandioca (Manihot esculenta com espécies florestais: Eucalyptus citriodora e Eucalyptus camaldulensis. A rentabilidade econômica do sistema foi mensurada, utilizando Valor Presente Líquido (VPL, Razão Benefício/Custo (RB/C para as culturas agrícolas anuais e Valor Esperado da terra (VET para os componentes madeireiros. Para tais cálculos foi escolhida uma taxa de desconto de 6%. O estudo demonstrou que o sistema agroflorestal apresentou rentabilidade econômica positiva, e que podem ser adotados por pequenos produtores. Assim, pode-se concluir que os sistemas agroflorestais são economicamente viáveis para pequenos produtores rurais trabalhando nas mesmas condições apresentadas neste estudo.

  11. 4种乡土植物幼苗抗旱性评价%Evaluation of drought resistance of four native plants seedlings

    Institute of Scientific and Technical Information of China (English)

    裴彩霞; 陈平; 刘金荣; 于得水

    2010-01-01

    采用盆栽法选择土壤含水量作为干旱胁迫的定量评价标准,叶片相对含水量及水分饱和亏缺、质膜相对透性、净光合速率(Pn)、气孔导度(Cond)、胞间CO_2:浓度(Co和蒸腾速率(Tr)等作为研究抗旱适应性的评价指标,研究干旱胁迫下麻疯树(Jatropha curcas L)、坡柳(Dodonaea viscose)、木豆(Cajanus cajan Millsp)、山毛豆(Tephrosia candida)的生理表现及光合特性.结果表明.干旱胁迫后,4种植物的叶片相对含水量下降,质膜相对透性增大,光合作用受到影响.综合各项指标及运用隶属函数分析得出4种植物的抗旱性依次为麻疯树>坡柳>木豆>山毛豆.

  12. Isoflavones hydrolisis and extraction

    Directory of Open Access Journals (Sweden)

    Jozilene Fernandes Farias dos Santos

    2012-12-01

    Full Text Available Isoflavones are found in leguminous species and are used as phytoestrogens widely used by industry for its beneficial effects as estrogens mimicked, antioxidant action and anti-cancer activity. The identification and quantification of isoflavones in plants is a need due to the high demand of industry. Several methods are used for its extraction, using organic solvents (methanol, ethanol and acetonitrile. Samples from five legumes species from Instituto de Zootecnia (IZ, Forage Gene Bank were tested. All seeds received a hydrothermic treatment immersed in pure water at 50°C for 12 hours. Seeds were then oven-dryed. In this work we tested the extraction using only the hydrothermic treatment and hyfrothermic treatment allied to methanol extaction protocol. Seeds were grinded and half of the samples were ressuspended in PBS (phosphate Buffer and the other half were submited to 4 mL of methanol and 1% of acetic acid, soaked for 5 hours, shaked every 15 minutes, at room temperature. The five legume species that we quantify isoflavones by enzyme immunoassay (EIA were: Calopogonium mucunoides, Bauhinia sp., Cajanus cajan, Galactia martii, Leucaena leucocephala. The extraction procedure is a recomendation of AOAC (Association of Official Analytical Chemists for isoflavone quantification. Ours results show an increase of extraction using methanol 80% plus acetic acid 1% and was obtained using solvent extraction in comparison to hydrothermic procedure alone (figure 1.

  13. An extra early mutant of pigeonpea

    International Nuclear Information System (INIS)

    The redgram (Cajanus cajan (L.) Huth) variety 'Prabhat DT' was gamma irradiated with 100, 200, 300 and 400 Gy doses. Several mutants have been identified viz., extra early mutants, monostem mutants, obcordifoliate mutants and bi-stigmatic mutants. The extra early mutant was obtained when treated with 100 Gy dose. The mutant was selfed and forwarded from M2 to M4 generation. In the M4 generation the mutant line was raised along with the parental variety. Normal cultural practices were followed and the biometrical observations were recorded. It was observed that for the characters viz., total number of branches per plant, number of pods per plants, seeds per pod, 100 seed weight and seed yield per plant there was no difference between the mutant and parent variety. Whereas, regarding the days to flowering and maturity the mutants were earlier than the parents. The observation was recorded from two hundred plants each. The mutant gives the same yield in 90 days as that of the parent variety in 107 days, which make it an economic mutant

  14. Weed suppression by green manure in an agroecological system

    Directory of Open Access Journals (Sweden)

    Katia Maria Garicoix Recalde

    2015-12-01

    Full Text Available ABSTRACT Green manure promotes efficient suppression of weeds, but green manure species can exhibit distinct behaviors, depending on the environmental conditions. This study aimed to evaluate the potential of soil mulching and weed suppression by spring/summer green manure species grown in the spring/summer season, at different growth stages and after management (cut, for 90 days during the cassava crop cycle. The study was carried out in the 2010/2011 season, in a system managed under agroecological principles. The treatments consisted of different green manure species and arrangements: Crotalaria juncea, Cajanus cajan, Canavalia brasiliensis, Canavalia ensiformis, Pennisetum americanum, Crotalaria juncea and Pennisetum americanum intercropped; Mucuna aterrima, Sorghum bicolor, a mixture of all the green manures in study and a control plot under fallow. The experiment was arranged in a randomized block design with four replications. The evaluations of the soil cover either by the green manures or weeds were performed at 45, 90 and 105 days after the emergence of the green manures. The cassava crop was planted under reduced tillage system at 11 days after the cut of the green manures. The percentage of soil covered by weeds and the dry matter produced were evaluated at 30, 60 and 90 days after planting. The results showed that the green manures had a suppressive effect on weeds during their life cycle, as well as during the first months after its management (cut, composing the mulch.

  15. The first genetic map of pigeon pea based on diversity arrays technology (DArT) markers

    Indian Academy of Sciences (India)

    Shi Ying Yang; Rachit A. Saxena; Pawan L. Kulwal; Gavin J. Ash; Anuja Dubey; John D. I. Harper; Hari D. Upadhyaya; Ragini Gothalwal; Andrzej Kilian; Rajeev K. Varshney

    2011-04-01

    With an objective to develop a genetic map in pigeon pea (Cajanus spp.), a total of 554 diversity arrays technology (DArT) markers showed polymorphism in a pigeon pea F2 mapping population of 72 progenies derived from an interspecific cross of ICP 28 (Cajanus cajan) and ICPW 94 (Cajanus scarabaeoides). Approximately 13% of markers did not conform to expected segregation ratio. The total number of DArT marker loci segregating in Mendelian manner was 405 with 73.1% ($P \\gt 0.001$) of DArT markers having unique segregation patterns. Two groups of genetic maps were generated using DArT markers. While the maternal genetic linkage map had 122 unique DArT maternal marker loci, the paternal genetic linkage map has a total of 172 unique DArT paternal marker loci. The length of these two maps covered 270.0 cM and 451.6 cM, respectively. These are the first genetic linkage maps developed for pigeon pea, and this is the first report of genetic mapping in any grain legume using diversity arrays technology.

  16. Medicinal plants of India with anti-diabetic potential.

    Science.gov (United States)

    Grover, J K; Yadav, S; Vats, V

    2002-06-01

    Since ancient times, plants have been an exemplary source of medicine. Ayurveda and other Indian literature mention the use of plants in treatment of various human ailments. India has about 45000 plant species and among them, several thousands have been claimed to possess medicinal properties. Research conducted in last few decades on plants mentioned in ancient literature or used traditionally for diabetes have shown anti-diabetic property. The present paper reviews 45 such plants and their products (active, natural principles and crude extracts) that have been mentioned/used in the Indian traditional system of medicine and have shown experimental or clinical anti-diabetic activity. Indian plants which are most effective and the most commonly studied in relation to diabetes and their complications are: Allium cepa, Allium sativum, Aloe vera, Cajanus cajan, Coccinia indica, Caesalpinia bonducella, Ficus bengalenesis, Gymnema sylvestre, Momordica charantia, Ocimum sanctum, Pterocarpus marsupium, Swertia chirayita, Syzigium cumini, Tinospora cordifolia and Trigonella foenum graecum. Among these we have evaluated M. charantia, Eugenia jambolana, Mucuna pruriens, T. cordifolia, T. foenum graecum, O. sanctum, P. marsupium, Murraya koeingii and Brassica juncea. All plants have shown varying degree of hypoglycemic and anti-hyperglycemic activity. PMID:12020931

  17. DEVELOPMENT AND PERFORMANCE EVALUATION OF TRACTOR FRONT MOUNTED PIGEON PEA STEM CUTTER

    Directory of Open Access Journals (Sweden)

    Atul R. Dange

    2010-07-01

    Full Text Available Pigeon pea or tur (Cajanus cajan L. Mills. is one of the important pulse crops of India and ranks second to chickpea in area and production. Traditionally the harvesting of pigeon pea is done manually by sickle, which demands considerable amount of labour, drudgery, time and cost to harvest, which reflects on total production cost of the crop. In view of this a tractor operated front mounted pigeon pea stem cutter was developed and being front mounted implement it facilitated better visibility and control to operator. The power was transmitted from pto to gear box. Arrangement of hydraulic cylinder and hydraulic motor was provided on the equipment to facilitate the height of cut and to rotate the conveyer belt. During comparative performance evaluation of developed equipment, the average cutting efficiency and field capacity was found 96.30 % and 0.176 ha/hr respectively. There was increase in fuel consumption and plant damage with increase in speed of operation. The average operation cost of newly developed tractor operated front mounted pigeon pea stem cutter was 64.71% less as compared with manual harvesting of pigeon pea crop. The time saved was almost 1/3rd to that of manual harvesting.

  18. Biological fixation and nitrogen transfer by three legume species in mango and soursop organic orchards;Fixacao biologica e transferencia de nitrogenio por leguminosas em pomar organico de mangueira e gravioleira

    Energy Technology Data Exchange (ETDEWEB)

    Paulino, Gleicia Miranda; Barroso, Deborah Guerra, E-mail: gleiciamiranda@yahoo.com.b, E-mail: deborah@uenf.b [Universidade Estadual do Norte Fluminense (UENF), Campos dos Goytacazes, RJ (Brazil). Dept. de Fitotecnia; Alves, Bruno Jose Rodrigues; Urquiaga, Segundo; Espindola, Jose Antonio Azevedo, E-mail: bruno@cnpab.embrapa.b, E-mail: urquiaga@cnpab.embrapa.b, E-mail: jose@cnpab.embrapa.b [EMBRAPA Agrobiologia, Seropedica, RJ (Brazil)

    2009-12-15

    The objective of this work was to evaluate the biological nitrogen fixation (BNF) and the N transfer derived from BNF of the legume species - Gliricidia sepium (gliricidia), Crotalaria juncea (sunnhemp) and Cajanus cajan (pigeon pea) - for an intercropped organic orchard with mango and soursop, through the {sup 15}N natural abundance method. The following inter cropping systems were evaluated: mango and soursop with gliricidia; mango and soursop with sunnhemp; mango and soursop with pigeon pea; and mango and soursop as control. Gliricidia showed the highest BNF potential (80%) , followed by sunnhemp (64.5%) and pigeon pea (45%). After two sunnhemp prunes, 149.5 kg ha{sup -1} of N per year were supplied, with 96.5 kg derived from BNF. After three annual prunes, gliricidia supplied 56.4 and 80.3 kg ha{sup -1} of N per year, with 45 and 64 kg derived from BNF, in two consecutive years. The quantity of N supplied to the system was higher than the mango and soursop requirements. Variations in the natural abundance of {sup 15}N were found only in soursop leaves. Gliricidia and sunnhemp were prominent in N transfer, with approximately 22.5 and 40% respectively. Green manuring using gliricidia permits fractioning of the N supply, which is an advantage in N obtention by the fruit trees (author)

  19. Diversity of root nodule bacteria from leguminous crops

    Directory of Open Access Journals (Sweden)

    Agrawal Pooja

    2016-01-01

    Full Text Available In the present study, a total of 353 nodule-associated bacteria were isolated from 220 legume plant samples belonging to Cicer arietinum (85, Glycine max (74, Vigna radiata (21 and Cajanus cajan (40. A total of 224 bacteria were identified as fast-growing Rhizobium spp. on the basis of differential staining (Gram staining and carbol fuchsin staining and biochemical tests. All the isolates were tested for indole acetic acid production (IAA, phosphate solubilization and siderophore production on plate assay. To examine the effect of volatile organic metabolites (VOM and water soluble soil components (WSSC on nodule bacteria, culture conditions were optimized by observing the effects of various parameters such as pH, salt content and temperatures on the growth of bacteria. Selected rhizobia were subjected to random amplified polymorphic DNA (RAPD and amplified ribosomal DNA restriction analysis (ARDRA analysis to identify their species. On the basis of RAPD and ARDRA, 10 isolates were identified as Rhizobium meliloti. In this study, Rhizobium GO4, G16, G20, G77, S43, S81, M07, M37, A15 and A55 were observed as the best candidates among the tested bacteria and can be further used as potent bioinoculants.

  20. Identification of lipoxygenase (LOX) genes from legumes and their responses in wild type and cultivated peanut upon Aspergillus flavus infection

    Science.gov (United States)

    Song, Hui; Wang, Pengfei; Li, Changsheng; Han, Suoyi; Lopez-Baltazar, Javier; Zhang, Xinyou; Wang, Xingjun

    2016-01-01

    Lipoxygenase (LOX) genes are widely distributed in plants and play crucial roles in resistance to biotic and abiotic stress. Although they have been characterized in various plants, little is known about the evolution of legume LOX genes. In this study, we identified 122 full-length LOX genes in Arachis duranensis, Arachis ipaënsis, Cajanus cajan, Cicer arietinum, Glycine max, Lotus japonicus and Medicago truncatula. In total, 64 orthologous and 36 paralogous genes were identified. The full-length, polycystin-1, lipoxygenase, alpha-toxin (PLAT) and lipoxygenase domain sequences from orthologous and paralogous genes exhibited a signature of purifying selection. However, purifying selection influenced orthologues more than paralogues, indicating greater functional conservation of orthologues than paralogues. Neutrality and effective number of codons plot results showed that natural selection primarily shapes codon usage, except for C. arietinum, L. japonicas and M. truncatula LOX genes. GCG, ACG, UCG, CGG and CCG codons exhibited low relative synonymous codon usage (RSCU) values, while CCA, GGA, GCU, CUU and GUU had high RSCU values, indicating that the latter codons are strongly preferred. LOX expression patterns differed significantly between wild-type peanut and cultivated peanut infected with Aspergillus flavus, which could explain the divergent disease resistance of wild progenitor and cultivars. PMID:27731413

  1. Agro-forestry practices and sustainable agriculture in yam producing communities of Niger state, Nigeria

    Directory of Open Access Journals (Sweden)

    Alamu L.O.

    2013-07-01

    Full Text Available In recognition of farmland burden in terms of land degradation, bulky nature of and unavailability of inorganic fertilizers as well as time constraint in the formation of organic fertilizers, there is the need to shift to agro-forestry practices. The practice will make room for arable crop production and forest/tree crop production. Four autonomous communities were studied in Niger State Nigeria. The communities are generally known for yam production. One hundred and twenty farmers were randomly selected for the study in Koro, Gwari, Kadara and Kambari communities. Thirty yam farmers were selected in each community using random sampling technique. One hundred and twenty farmers were studied in all. Results were analysed using simple percentages and pie charts. Results show that there is a high level of consciousness of agro-forestry among the farmers. Shrubs such as Gliricidia sepium and Cajanus cajan are common and they make a cumulative percentage of 35.0% of tree species used for agro- forestry practices. Fruit trees such as Citrus species make a cumulative percentage of 31.0% while other tree species used includes but not exclusive to Tectona grandis, Gmelina arborea and Terminalia species. It is recommended that youths and young farmers in the communities be more involved in agro-forestry practices of this nature for a sustainable practice to be ensured.

  2. Pigeonpea hybrid-proline-rich protein (CcHyPRP confers biotic and abiotic stress tolerance in transgenic rice

    Directory of Open Access Journals (Sweden)

    Venkateswara Rao eKhareedu

    2016-01-01

    Full Text Available In this study, we report the overexpression of Cajanus cajan hybrid-proline-rich protein encoding gene (CcHyPRP in rice which resulted in increased tolerance to both abiotic and biotic stresses. Compared to the control plants, the transgenic rice lines, expressing CcHyPRP, exhibited high-level tolerance against major abiotic stresses, viz., drought, salinity and heat, as evidenced by increased biomass, chlorophyll content, survival rate, root and shoot growth. Further, transgenic rice lines showed increased panicle size and grain number compared to the control plants under different stress conditions. The CcHyPRP transgenics, as compared to the control, revealed enhanced activities of catalase and superoxide dismutase (SOD enzymes and reduced malondialdehyde (MDA levels. Expression pattern of CcHyPRP::GFP fusion-protein confirmed its predominant localization in cell walls. Moreover, the CcHyPRP transgenics, as compared to the control, exhibited increased resistance to the fungal pathogen Magnaporthe grisea which causes blast disease in rice. Higher levels of bZIP and endochitinase transcripts as well as endochitinase activity were observed in transgenic rice compared to the control plants. The overall results demonstrate the intrinsic role of CcHyPRP in conferring multiple stress tolerance at the whole-plant level. The multipotent CcHyPRP seems promising as a prime candidate gene to fortify crop plants for enhanced tolerance/resistance to different stress factors.

  3. Composição química de grãos de feijao-guandu cultivar kaki Chemical composition of pigeon pea seeds

    Directory of Open Access Journals (Sweden)

    João Paulo Feijão Teixeira

    1985-01-01

    Full Text Available Foram analisadas sementes de tegumento creme e alaranjado de feijão-guandu (Cajanus cajan (L. Milisp., oriundas do cultivar Kaki, quanto aos teores de proteína, extrato etéreo, açúcares solúveis e polissacarídeos. Os dois tipos de sementes apresentaram composição química similar. Os teores médios encontrados foram: proteína 23,11%, extrato etéreo 1,34%, açúcar solúvel 9,46% e polissacarídeos 16,77%. A fração protéica apresentou baixos níveis de aminoácidos sulfurados, isoleucina, valina e treonina.Pigeon pea seeds, cv' Kaki either with light-yellow or orange testa pigments were analysed for protein, ether extract, total sugar and polysaccharides. Similar results were obtained for the two types of seeds. On the average the seeds had the following contents: protein - 23.11%; ether extract - 1.34%; total sugar - 9.46%; polysaccharides - 16.77%. The protein fraction had low levels of sulfured aminoacids, isoleucine, valine and threonine.

  4. Erosão em entressulcos sob caatinga e culturas agrícolas Interrill erosion under shrub and crop systems

    Directory of Open Access Journals (Sweden)

    Francisco José de Freitas

    2008-08-01

    Full Text Available Dentre as formas de erosão hídrica do solo, a erosão em entressulcos é uma das que causam maiores danos no processo produtivo dos solos utilizados na agricultura, por provocar perdas de solo, água e nutrientes. Frente a isso, o presente trabalho visou avaliar a proteção ambiental promovida pela cobertura vegetal da caatinga, do feijão-guandu (Cajanus cajan e da batata-doce (Ipomoea batatas Lam em relação ao solo descoberto, bem como o efeito das práticas de cultivos dessas culturas, por meio das taxas da erosão em entressulcos e das características hidráulicas do escoamento superficial vinculado à erosão em entressulcos, em um Luvissolo. Para isso, foi implementada uma série de 20 chuvas simuladas em Serra Talhada, município do semi-árido do Estado de Pernambuco, sob os seguintes tratamentos: (1 solo descoberto; (2 solo coberto pela cultura do feijão-guandu (Cajanus cajan; (3 solo coberto pela cultura da batata-doce (Ipomoea batatas Lam.; (4 solo coberto por Caatinga semi-arbustiva. Todos os regimes de escoamento superficial obtidos foram laminar lento. As coberturas vegetais proporcionadas pela Caatinga e pelo guandu, que deram origem aos maiores valores de cobertura do solo, responderam pelas menores taxas de concentração de sedimentos e desagregação do solo na erosão em entressulcos, em decorrência da maior rugosidade hidráulica da superfície do solo promovida por essas vegetações. As taxas de concentração de sedimentos no cultivo da batata-doce foram iguais às do solo descoberto, por conta do revolvimento do solo na preparação das leiras de plantio, e determinou as maiores taxas de desagregação do solo entre as coberturas vegetais; em contrapartida, as leiras permitiram retenção do escoamento superficial. As coberturas vegetais Caatinga, guandu e batata-doce e as respectivas rugosidades hidráulicas impostas ao escoamento superficial determinaram reduções exponenciais das perdas de solo. A cobertura

  5. Recuperação de área degradada por construção de hidroelétrica com adubação verde e corretivo Soil reclamation by green manure and soil ammendements in areas degraded by hydroelectric power plant construction

    Directory of Open Access Journals (Sweden)

    Marlene Cristina Alves

    2008-12-01

    system in Ilha Solteira, São Paulo, Brazil, by green manure, lime and gypsum. The experiment had a randomized block design with seven treatments and four replications. The treatments consisted of: one control (tilled soil and spontaneous vegetation; Cajanus cajan until 1994 and then substituted by Canavalia ensiformis; lime + Stizolobium aterrimum; lime + Cajanus cajan until 1994 and then substituted by Canavalia ensiformis; lime + gypsum + Stizolobium aterrimum and lime + gypsum + Cajanus cajan until 1994 and then substituted by Canavalia ensiformis. After four years with green manure, one year with corn, one year with Avena strigosa and two years with brachiaria, the following properties were analyzed: pH, Ca, Mg, P, K, cation exchange capacity, base saturation, and organic matter. The treatments succeeded in recovering the soil chemical properties. Stizolobium aterrimum performed better than Cajanus cajan and Canavalia ensiformis. Soil recovery effects reached a depth of 0.0-0.2 m. The recovery techniques (green manure, lime and gypsum improved soil chemical characteristics to a of 0.0-0.1 m in the first year and only after five years the depth of 0.0-0.2 m.

  6. Matéria orgânica em um Latossolo submetido a diferentes sistemas de produção de milho Soil organic matter in an oxisol under different corn production systems

    Directory of Open Access Journals (Sweden)

    W. J. O. Souza

    2003-12-01

    Full Text Available Com objetivo de avaliar o efeito de sistemas de produção sobre a dinâmica do carbono no solo e nas frações da matéria orgânica, durante seis anos, em Jaboticabal (SP, foram testados diferentes tratamentos, a saber: semeadura convencional de milho com pousio no inverno (C-Mi-P, plantio direto de milho e pousio no inverno (D-Mi-P, convencional de milho em rotação com soja e pousio no inverno (C-Mi-P-So, plantio direto de milho em rotação com soja e pousio no inverno (D-Mi-P-So e plantio direto de milho com uso de Mucuna aterrina (mucuna preta, Cajanus cajan (feijão guandu e Crotalaria juncea no inverno (D-Mi-Mu, D-Mi-Gu e D-Mi-Cr em delineamento de blocos ao acaso e parcelas subdivididas. Após 60 dias da emergência das plântulas, coletaram-se amostras de solo a diferentes profundidades (0-0,05, 0,05-0,10 e 0,10-0,20 m. Avaliaram-se nas amostras os teores de C orgânico (CO e C total nas frações: matérias húmicas (MH, solúvel em água (SA, ácido fúlvico (AF, ácido húmico (AH e humina (HN com dados expressos em base de terra seca em estufa (TSE. O uso do sistema plantio direto com pousio e o cultivo de leguminosas no inverno (mucuna preta e feijão guandu foram os que apresentaram maiores valores de CO e C-HN na camada de 0-0,05 m. O tratamento C-Mi-P-So mostrou o maior valor de C-SA na camada de 0,05-0,10 m. Quanto aos teores de C-MH, os tratamentos C-Mi-P-So e D-Mi-P-So foram os que apresentaram os maiores valores nas camadas superficiais. O plantio direto de milho em monocultura e sucessão com leguminosas (mucuna preta e feijão guandu parecem ter favorecido dois processos: migração de AF para as camadas mais profundas, reduzindo os valores de MH nas camadas superficiais do solo, e interconversões de AF em AH mais rápidas.A long-term field trial was carried out in the Jaboticabal county, São Paulo State. with the objective of estimating production system effects on the soil organic carbon and soil organic matter

  7. Late Embryogenesis Abundant (LEA proteins in legumes

    Directory of Open Access Journals (Sweden)

    Marina eBattaglia

    2013-06-01

    Full Text Available Plants are exposed to different external conditions that affect growth, development, and productivity. Water deficit is one of these adverse conditions caused by drought, salinity, and extreme temperatures. Plants have developed different responses to prevent, ameliorate or repair the damage inflicted by these stressful environments. One of these responses is the activation of a set of genes encoding a group of hydrophilic proteins that typically accumulate to high levels during seed dehydration, at the last stage of embryogenesis, hence named Late Embryogenesis Abundant (LEA proteins. LEA proteins also accumulate in response to water limitation in vegetative tissues, and have been classified in seven groups based on their amino acid sequence similarity and on the presence of distinctive conserved motifs. These proteins are widely distributed in the plant kingdom, from ferns to angiosperms, suggesting a relevant role in the plant response to this unfavorable environmental condition. In this review, we analyzed the LEA proteins from those legumes whose complete genomes have been sequenced such as Phaseolus vulgaris, Glycine max, Medicago truncatula, Lotus japonicus, Cajanus cajan and Cicer arietinum. Considering their distinctive motifs, LEA proteins from the different groups were identified, and their sequence analysis allowed the recognition of novel legume specific motifs. Moreover, we compile their transcript accumulation patterns based on publicly available data. In spite of the limited information on these proteins in legumes, the analysis and data compiled here confirms the high correlation between their accumulation and water deficit, reinforcing their functional relevance under this detrimental conditions.

  8. Allelopathic effects of leaf litters of Eucalyptus camaldulensis on some forest and agricultural crops

    Institute of Scientific and Technical Information of China (English)

    Romel Ahmed; A. T. M. Rafiqul Hoque; Mohammed Kamal Hossain

    2008-01-01

    Allelopathic effects of different doses of Eucalyptus camaldulensis leaf litters were investigated through an experiment in the green house of Institute of Forestry and Environmental Sciences, Chittagong, Bangladesh. Three popular agricultural crops: Falen (Vigna unguiculata), Chickpea (Cicer arietinum), Arhor (Cajanus cajan) and two widely used plantation trees: Sada koroi (Albizia procera) and Ipil ipil (Leucaena leucocephala) were selected as bioassay species. Experiment was set on tray at room temperature 27℃. The effects of different doses of leaf litter extracts were compared to the control. Results suggest that leaf litters of E. camaldulesis induced inhibitory effects. It was also found that the effect depend on concentration of extract and litterfall, type of receiver species. Higher concentration of the materials had the higher effect and vice versa. Though all the bioassay species were suppressed some of them showed better performance. Vigna unguiculata, Cicer arietinum are recommended in agroforestry based on this present Experiment output. In mixed plantation, Leucaena leucochephala is a better choice while compared to Albizia procera.

  9. Feeding and oviposition preference of Phyllophaga cuyabana (Moser) (Coleoptera: Melolonthidae) on several crops

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Lenita J.; Hoffmann-Campo, Clara B. [EMBRAPA Soja, Londrina, PR (Brazil). Centro Nacional de Pesquisa de Soja]. E-mail: lenita@cnpso.embrapa.br; Garcia, Maria A. [Universidade Estadual de Campinas (UNICAMP), Campinas, SP (Brazil). Inst. de Biologia. Dept. de Zoologia; Amaral, Maria L.B. do [Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brasilia, DF (Brazil)

    2007-09-15

    Laboratory and greenhouse experiments were carried out to study food and oviposition preference by Phyllophaga cuyabana (Moser) on different plant species as Cajanus cajan L. (pigeon pea), Crotalaria juncea L. (sun hemp), Crotalaria spectabilis Roth (showy crotalaria), Crotalaria ochroleuca G. Don (slenderleaf rattlebox), Glycine max [L.] Merrill (soybean), Gossypium hirsutum L. (cotton), Helianthus annuus L. (sunflower), Stizolobium aterrimum [Mucuna aterrima] Piper and Tracey (velvetbean) and Zea mays L. (mayze). In no-choice experiments, the number of eggs layed in sunflower, C. juncea and soybean was larger compared to cotton. Despite the fact that the adults did not discriminate among plants, in dual-choice test, the proportion of eggs layed and leaf consumption by P. cuyabana adults in soybean were significantly higher than in C. spectabilis. The larval distribution in the soil was at random in multiple-choice, without any trend of preference, but in dual-choice, when soybean was the control, larvae always preferred to feed on its roots. P. cuyabana adults had preference for more suitable hosts and that could stand their offspring survival. This behaviour can be usefully exploited in an integrated management program for this pest. (author)

  10. Development of cytoplasmic-nuclear male sterility, its inheritance, and potential use in hybrid pigeonpea breeding.

    Science.gov (United States)

    Saxena, Kul B; Ravikoti, V Kumar; Dalvi, Vijay A; Pandey, Lalji B; Gaddikeri, Guruprasad

    2010-01-01

    Pigeonpea [Cajanus cajan (L.) Millsp.] is a unique food legume because of its partial (20-30%) outcrossing nature, which provides an opportunity to breed commercial hybrids. To achieve this, it is essential to have a stable male-sterility system. This paper reports the selection of a cytoplasmic-nuclear male-sterility (CMS) system derived from an interspecific cross between a wild relative of pigeonpea (Cajanus sericeus Benth. ex. Bak.) and a cultivar. This male-sterility source was used to breed agronomically superior CMS lines in early (ICPA 2068), medium (ICPA 2032), and late (ICPA 2030) maturity durations. Twenty-three fertility restorers and 30 male-sterility maintainers were selected to develop genetically diverse hybrid combinations. Histological studies revealed that vacuolation of growing tetrads and persistence of tetrad wall were primary causes of the manifestation of male sterility. Genetic studies showed that 2 dominant genes, of which one had inhibitory gene action, controlled fertility restoration in the hybrids. The experimental hybrids such as TK 030003 and TK 030009 in early, ICPH 2307 and TK 030625 in medium, and TK 030861 and TK 030851 in late maturity groups exhibited 30-88% standard heterosis in multilocation trials.

  11. Phenology of Migration and Decline in Colony Numbers and Crop Hosts of Giant Honeybee (Apis dorsata F. in Semiarid Environment of Northwest India

    Directory of Open Access Journals (Sweden)

    Ram Chander Sihag

    2014-01-01

    Full Text Available The colonies of the giant honeybee (Apis dorsata immigrate in the semiarid environment of Northwest India in October-November with the onset of flowering on pigeon pea (Cajanus cajan/toria (Brassica campestris var. toria, stay here during the rich pollen and nectar flow period from December to mid-May, and emigrate in late May/early June when floral dearth is witnessed. This honeybee was free from any conspicuous viral, bacterial, and fungal diseases and also did not have any serious predators and enemies. However, about 20 percent of the old colonies were infested with Tropilaelaps clareae and 100 percent of the old colonies with Galleria mellonella; none of the swarm colonies had these pests. While the migration schedule of this honeybee remained similar year after year, the number of colonies immigrating in this region declined markedly over the years; the number in 2012 was even less than half of that recorded in 1984. During its stay in this region, this honeybee acted as an important pollinator of more than 30 crop plants of this region. The causes of seasonal migration and decline in the number of colonies of this honeybee and its importance in crop pollination have been discussed.

  12. Siderophore as a potential plant growth-promoting agent produced by Pseudomonas aeruginosa JAS-25.

    Science.gov (United States)

    Sulochana, M B; Jayachandra, S Y; Kumar, S Anil; Parameshwar, A B; Reddy, K Mohan; Dayanand, A

    2014-09-01

    Siderophores scavenges Fe(+3) from the vicinity of the roots of plants, and thus limit the amount of iron required for the growth of pathogens such as Fusarium oxysporum, Pythium ultimum, and Fusarium udum, which cause wilt and root rot disease in crops. The ability of Pseudomonas to grow and to produce siderophore depends upon the iron content, pH, and temperature. Maximum yield of siderophore of 130 μM was observed at pH 7.0 ± 0.2 and temperature of 30 °C at 30 h. The threshold level of iron was 50 μM, which increases up to 150 μM, favoring growth but drastically affecting the production of siderophore by Pseudomonas aeruginosa JAS-25. The seeds of agricultural crops like Cicer arietinum (chick pea), Cajanus cajan (pigeon pea), and Arachis hypogaea (ground nut) were treated with P. aeruginosa JAS-25, which enhanced the seed germination, root length, shoot length, and dry weight of chick pea, pigeon pea, and ground nut plants under pot studies. The efficient growth of the plants was not only due to the biocontrol activity of the siderophore produced by P. aeruginosa JAS-25 but also may be by the production of indole acetic acid (IAA), which influences the growth of the plants as phytohormones.

  13. Environmental influences on pigeonpea-Fusarium udum interactions and stability of genotypes to Fusarium wilt.

    Directory of Open Access Journals (Sweden)

    Mamta eSharma

    2016-03-01

    Full Text Available Fusarium wilt (Fusarium udum Butler is an important biotic constraint to pigeonpea (Cajanus cajan L. production worldwide. Breeding for fusarium wilt resistance continues to be an integral part of genetic improvement of pigeonpea. Therefore, the study was aimed to identify and validate resistant genotypes to fusarium wilt and determine the magnitude of genotype × environment (G × E interactions through multi-environment and multi-year screening. Total 976 genotypes including germplasm and breeding lines were screened against wilt in wilt sick plot at Patancheru, India. Ninety two genotypes found resistant to wilt were tested further for two more years in wilt sick plot at Patancheru and Pigeonpea Wilt Nursery (PWN comprising of 29 genotypes was constituted. PWN was evaluated at nine locations representing different agro-climatic zones of India for wilt resistance during two crop seasons 2007/08 and 2008/09. Genotypes (G, environment (E and G × E interactions biplot partitioned main effect into G, E and G × E interactions and significant effects (p≤0.001 were obtained for wilt incidence. Genotype contributed 36.51% of resistance variation followed by environment (29.32%. GGE biplot in integration with boxplot and multiple comparison tests enabled us to identify seven stable genotypes (ICPL 20109, ICPL 20096, ICPL 20115, ICPL 20116, ICPL 20102, ICPL 20106 and ICPL 20094 based on their performance across diverse environments. These genotypes have broad based resistance and can be exploited in pigeonpea breeding program.

  14. Bioindicators of soil quality in coffee organic cultivation systems

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    Márcio Sampaio Pimentel

    2011-05-01

    Full Text Available The objective of this work was to assess the effect of different coffee organic cultivation systems on chemical and biological soil characteristics, in different seasons of the year. The following systems were evaluated: coffee intercropped with one (CJ1, two (CJ2 or three (CJ3 pigeon pea (Cajanus cajan alleys; coffee planted under full sun (CS; area planted with sweet pepper and snap bean in a conventional tillage system (AC; and secondary forest area (FFR. Row spacing in CJ1, CJ2, CJ3 and CS was 2.0x1.0, 2.8x1.0, 3.6x1.0, and 2.8x1.0 m, respectively. Soil samples were collected at 10-cm depth, during the four seasons of the year. The results were subjected to analysis of variance, principal component analysis, and redundancy analysis. There was an increase in edaphic macrofauna, soil basal respiration, and microbial quotient in the summer. Total macrofauna density was greater in CJ2 followed by CJ3, CS, CJ1, AC and FFR; Coleoptera, Formicidae, and Isoptera were the most abundant groups. There are no significant differences among the areas for soil basal respiration, and the metabolic quotient is higher in CJ1, CJ3, and FFR. Microbial biomass carbon and the contents of K, pH, Ca+Mg, and P show greater values in AC.

  15. Emaravirus: A Novel Genus of Multipartite, Negative Strand RNA Plant Viruses

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    Hans-Peter Mühlbach

    2012-09-01

    Full Text Available Ringspot symptoms in European mountain ash (Sorbus aucuparia L., fig mosaic, rose rosette, raspberry leaf blotch, pigeonpea sterility mosaic (Cajanus cajan and High Plains disease of maize and wheat were found to be associated with viruses that share several characteristics. They all have single-stranded multipartite RNA genomes of negative orientation. In some cases, double membrane-bound virus-like particles of 80 to 200 nm in diameter were found in infected tissue. Furthermore, at least five of these viruses were shown to be vectored by eriophyid mites. Sequences of European mountain ash ringspot-associated virus (EMARaV, Fig mosaic virus (FMV, rose rosette virus (RRV, raspberry leaf blotch virus (RLBV, pigeonpea sterility mosaic virus and High Plains virus strongly support their potential phylogenetic relationship. Therefore, after characterization of EMARaV, the novel genus Emaravirus was established, and FMV was the second virus species assigned to this genus. The recently sequenced RRV and RLBV are supposed to be additional members of this new group of plant RNA viruses.

  16. Quantifying below-ground nitrogen of legumes: Optimizing procedures for 15N shoot-labelling

    International Nuclear Information System (INIS)

    Quantifying below-ground nitrogen (N) of legumes is fundamental to understanding their effects on soil mineral N fertility and on the N economies of following or companion crops in legume-based rotations. Methodologies based on 15N-labelling of whole plants with subsequent measurement of 15N in recovered plant parts and in the root-zone soil have proved promising. We report four glasshouse experiments with objectives to develop appropriate protocols for in situ 15N labelling of four pulses, faba bean (Vicia faba), chickpea (Cicer arietinum), mung bean (Vigna radiata) and pigeon pea (Cajanus cajan). Treatments included 15N-urea concentration, feeding technique, leaflet/petiole position, and frequency of feeding. Nitrogen-15-labelling via the leaf-flap was best for faba bean, mung and pigeon pea, whilst petiole feeding was best for chickpea, in all cases using 0.2-mL volumes of 0.5% urea (98 atom% 15N excess). The implications of uneven enrichment of the nodulated roots because of effects of the 15N-depleted nodules when calculating root-derived N in soil are discussed. (author)

  17. Pigeonpea Hybrid-Proline-Rich Protein (CcHyPRP) Confers Biotic and Abiotic Stress Tolerance in Transgenic Rice.

    Science.gov (United States)

    Mellacheruvu, Sunitha; Tamirisa, Srinath; Vudem, Dashavantha Reddy; Khareedu, Venkateswara Rao

    2015-01-01

    In this study, we report the overexpression of Cajanus cajan hybrid-proline-rich protein encoding gene (CcHyPRP) in rice which resulted in increased tolerance to both abiotic and biotic stresses. Compared to the control plants, the transgenic rice lines, expressing CcHyPRP, exhibited high-level tolerance against major abiotic stresses, viz., drought, salinity, and heat, as evidenced by increased biomass, chlorophyll content, survival rate, root, and shoot growth. Further, transgenic rice lines showed increased panicle size and grain number compared to the control plants under different stress conditions. The CcHyPRP transgenics, as compared to the control, revealed enhanced activities of catalase and superoxide dismutase (SOD) enzymes and reduced malondialdehyde (MDA) levels. Expression pattern of CcHyPRP::GFP fusion-protein confirmed its predominant localization in cell walls. Moreover, the CcHyPRP transgenics, as compared to the control, exhibited increased resistance to the fungal pathogen Magnaporthe grisea which causes blast disease in rice. Higher levels of bZIP and endochitinase transcripts as well as endochitinase activity were observed in transgenic rice compared to the control plants. The overall results demonstrate the intrinsic role of CcHyPRP in conferring multiple stress tolerance at the whole-plant level. The multipotent CcHyPRP seems promising as a prime candidate gene to fortify crop plants for enhanced tolerance/resistance to different stress factors. PMID:26834756

  18. Fitomassa e relação C/N em consórcios de sorgo e milho com espécies de cobertura Biomass and C/N ratio in intercrops of sorghum and maize with cover crops

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    Paulo Claudeir Gomes da Silva

    2009-11-01

    Full Text Available O objetivo deste trabalho foi quantificar a produtividade de fitomassa, o teor e acúmulo de nitrogênio (N, e a relação carbono/nitrogênio (C/N de monocultivos de sorgo (Sorghum bicolor e milho (Zea mays e de seus consórcios com guandu-anão (Cajanus cajan, crotalária (Crotalaria juncea, tremoço branco (Lupinus albus, girassol (Helianthus annuus e nabo-forrageiro (Raphanus sativus, manejados em diferentes estádios. O experimento foi conduzido de março a julho de 2008, em Argissolo Vermelho distroférrico de textura média, no sistema plantio direto. O delineamento experimental foi o de blocos completos ao acaso, com quatro repetições, e parcelas subdivididas, constituído pelos tratamentos: monocultivos de sorgo e milho e seus respectivos consórcios com guandu-anão, crotalária, girassol, nabo-forrageiro e tremoço branco, nas parcelas; e épocas de corte, aos 60, 90 e 120 dias após a semeadura nas subparcelas. Consórcios de sorgo e milho com outras espécies superaram expressivamente a produtividade de fitomassa de seus monocultivos que ainda acumularam menos N e apresentaram maiores relações C/N na fitomassa. Para aumentar a produtividade de fitomassa, a melhor época de corte é aos 120 dias após a semeadura das culturas de cobertura. O corte aos 90 dias após a semeadura propicia o maior acúmulo de N e as menores relações C/N.The objective of this work was to quantify the biomass production, the content and accumulation of nitrogen (N and the carbon/nitrogen (C/N relation in monocultures of sorghum (Sorghum bicolor and corn (Zea mays, and intercrops with pigeon pea (Cajanus cajan, sun hemp (Crotalaria juncea, white lupine (Lupinus albus, sunflower (Helianthus annuus and turnip forage (Raphanus sativus, managed in different stages. The experiment was carried out from March to July 2008, in a Haplic Acrisol, medium texture, under no-tillage. A randomized complete block design, with four replicates and split plots with the

  19. COVER CROPS EFFECTS ON THE DEVELOPMENT OF UPLAND RICE UNDER ORGANIC FARMING SYSTEM PLANTAS DE COBERTURA DE SOLO E SEUS EFEITOS NO DESENVOLVIMENTO DA CULTURA DO ARROZ DE TERRAS ALTAS EM CULTIVO ORGÂNICO

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    Roberta Paula de Jesus

    2007-12-01

    Full Text Available

    This research was conducted in an experimental area in Santo Antônio de Goiás (16º28'S, 49º17'W and altitude 823 m, Brazil, during the months of June, 2004, and March, 2005. The upland rice variety Aimoré was used along with several cover crops aiming to evaluate leaf area, number of tillers, dry matter, and nitrogen content in the phytomass during the rice crop cycle. The experimental design was the randomized blocks one, with five treatments and four replications. The treatments consisted of different cover crops, such as velvet bean (Mucuna aterrima, sunn hemp (Crotalaria juncea, dwarf pigeon pea (Cajanus cajan, broom sorghum (Sorghun bicolor, and a check treatment with spontaneous vegetation growing among the rice plants. The leguminous plants, specially C. juncea, presented better results in tillering production, leaf area index, dry matter yield, and accumulated nitrogen content, if compared to the treatments where grasses were used as soil cover crop. It was concluded that rice presented a satisfactory development in the different soil cover treatments, specially after leguminous cultivation.

    KEY-WORDS: Rice, green manure, leaf area index, dry matter.

    O experimento foi conduzido numa área experimental em Santo Antônio de Goiás (16º28'S, 49º17'W e altitude de 823 m, no período de junho de 2004 a março de 2005. Utilizou-se a cultivar Aimoré de arroz de terras altas, em seqüência a diferentes plantas de cobertura de solo, com o objetivo de avaliar o índice de área foliar (IAF, número de afilhos, acúmulo de massa de matéria seca (MMS e o teor de nitrogênio acumulado na fitomassa durante o ciclo da cultura do arroz. O delineamento experimental foi o de blocos ao acaso, com cinco

  20. Nitrogen-fixation Potential of Nodules in Four Types of Nitrogen-fixation Plants and Their Influencing Factors in Dry-hot Valley%干热河谷4种固氮植物根瘤固氮潜力及其影响因素

    Institute of Scientific and Technical Information of China (English)

    唐国勇; 李昆; 孙永玉; 张春华

    2012-01-01

    In addition to water, Nitrogen ( N) is often the key limiting factor for biological activity in Dry-hot Valleys, Biological N-fixation by nitrogen-fixation plants is of important source of N for vegetations in those areas. The nitrogenase activities (NAs) of nodules in Acacia auriliformis A. Cunn, Leucaena leucacephala (Lara. ) de Wit, Cajanus cajan ( L). Millspangh and Albiza kalkora Prain plantations were determined at the Dry red soils and Verti-sol spots at four different sampling times in a Dry-hot Valley with the acetylene reduction assay. The results showed that the NAs of nodules in L. leucacephala (16, 25 μmol·g -1·h-1 ) and A. auriliformis ( 15. 85μmol·g-1·h-1) were significantly higher than those in A. kalkora (9,60 μmol·g-1·h-1) and C. cajan (9.42 μmol·g-1h-1 )·The NAs of nodules in rainy season were significantly higher than those in dry season, and approximated 2. 3 times that in dry season. The NAs of nodules at the Dry red soils spots were 1.3 -1.6 times higher than those at the Vertisol spots. The research revealed besides plant type, the NAs of nodules were primarily affected by soil type, season and soil water content, but less affected by soil temperature.%氮是除水分之外影响干热河谷生物活性的关键因子,豆科植物生物固氮是该地区氮素的重要来源之一.采用乙炔还原法测定了干热河谷不同季节燥红土和变性土林地中大叶相思、新银合欢、木豆和山合欢根瘤固氮酶活性(NAs).结果表明:新银合欢(16.25 μmol · g-1·h-1)和大叶相思(15.85 μmol·g-1·h-1)根瘤NAs显著(P<0.001)高于山合欢(9.60 μmol·g-1·h-1)和木豆(9.42 μmol·g-1·h-1).雨季根瘤NAs显著高于旱季,约为旱季的2.3倍.燥红土样地上植物根瘤NAs是变性土样地的1.3~1.6倍.研究揭示:除植被类型外,干热河谷植物根瘤NAs主要受土壤类型、季节和土壤含水量的影响,而受土壤温度的影响较小.

  1. Efeito de pré-cultivos sobre o potencial de inóculo de fungos micorrízicos arbusculares e produção da mandioca Effect of pre-crops on the inoculum potential of arbuscular mycorrhizal fungi and cassava yield

    Directory of Open Access Journals (Sweden)

    Francisco Adriano de Souza

    1999-10-01

    Full Text Available Avaliou-se o efeito de pré-cultivos com adubos verdes sobre o potencial de inóculo de fungos micorrízicos arbusculares e produção de mandioca (Manihot esculenta, Crantz. O experimento foi conduzido no campo experimental da Embrapa-Centro Nacional de Pesquisa de Agrobiologia (CNPAB, em solo Podzólico Vermelho-Amarelo, localizado no Município de Seropédica, RJ, em parcelas experimentais de 30 m², dispostas em delineamento de blocos casualizados com quatro repetições. Os pré-cultivos constaram de Canavalia ensiformis, Mucuna aterrima, Crotalaria juncea, Cajanus cajan e Sorghum bicolor (BR 005 e o tratamento-controle (solo mantido sem plantas. Após um período de 82 dias de crescimento, sobre as mesmas parcelas cultivou-se mandioca, por oito meses. A produtividade de raízes de mandioca variou de 12,76 a 17,20 t ha-1, porém não foi detectada diferença estatística entre os tratamentos. Foram identificadas 16 espécies de fungos micorrízicos arbusculares e foi constatado que o número de esporos de algumas espécies sofreu alteração da sua freqüência de ocorrência com os cultivos. O pré-cultivo com sorgo aumentou o número de propágulos infectivos em relação ao controle, demonstrando que o uso de espécies de plantas com capacidade para elevar o potencial de inóculo pode ser um bom caminho alternativo para resolver o problema de produção de inóculo para culturas micotróficas em campo.The effect of pre-crops on the inoculum potential (IP of arbuscular mycorrhizal fungi (AMF and yield of cassava was evaluated on a Red-Yellow Podzolic soil in the field. The experiment was carried out at Embrapa-Centro Nacional de Pesquisa de Agrobiologia (CNPAB, Seropédica, RJ, Brazil, in plots with 30 m² arranged in a randomised block design with four replications. As pre-crops, four legume species were used: Canavalia ensiformis, Mucuna aterrina, Crotalaria juncea and Cajanus cajan; and a grass Sorghum bicolor (BR 005 in relation to

  2. Características químicas de solo e rendimento de fitomassa de adubos verdes e de grãos de milho, decorrente do cultivo consorciado Soil chemical characteristics and green manure yield in a corn intercropped system

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    Reges Heinrichs

    2005-02-01

    Full Text Available A adubação verde é uma das formas de aporte de matéria orgânica ao solo. O sistema de cultivo consorciado de culturas pode ser uma alternativa para aumentar a reciclagem de nutrientes e melhorar a produtividade. Para avaliar o sistema consorciado de adubos verdes com o milho, foram estudadas as características químicas do solo, a produção de matéria seca, a composição mineral de adubos verdes e o rendimento de grãos de milho, num experimento realizado em campo, entre 1995 e 1997, em solo classificado como Nitossolo Vermelho eutrófico. O milho foi semeado no espaçamento de 90 cm nas entrelinhas, perfazendo, aproximadamente, 50.000 plantas por hectare. Os tratamentos constaram de quatro espécies de adubos verdes: mucuna anã [Mucuna deeringiana (Bort. Merr], guandu anão (Cajanus cajan L., crotalária (Crotalaria spectabilis Roth e feijão-de-porco (Canavalia ensiformis L. e um tratamento-testemunha, sem cultivo consorciado. Essas espécies foram semeadas sem adubação, no meio da entrelinha, em duas épocas: simultânea ao milho e 30 dias após. O delineamento experimental foi o de blocos ao acaso em parcelas subdivididas, com quatro repetições. O feijão-de-porco apresentou maior produção de fitomassa e acúmulo de N, P, K, Ca, Mg e S. No primeiro ano de cultivo, o rendimento de grãos de milho não foi influenciado pelo cultivo consorciado com adubos verdes; no entanto, no segundo, a produção foi beneficiada pelo consórcio com feijão-de-porco.Green manure is one way of supplying organic matter to soil. The mixed cultivation of crops may be an alternative to increase nutrient cycling and to improve productivity. To evaluate intercrops of green manure and corn, soil chemical characteristics, green manure dry matter production and its mineral composition and corn yield were determined in a field experiment carried out between 1995 and 1997 on an Aleudalf Soil in Piracicaba, state of São Paulo, Brazil. Corn was sown in

  3. Crescimento e produtividade do sorgo forrageiro BR 601 sob adubação verde Growth and yield of forage sorghum cv. BR 601 under green manure

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    Romeu C. Andrade Neto

    2010-02-01

    Full Text Available O efeito da adubação verde sobre o crescimento e a produtividade do sorgo forrageiro BR 601, foi estudado em experimento desenvolvido na UFERSA, em Mossoró, RN, no qual o delineamento utilizado foi em blocos casualizados completos, em esquema de parcelas subdivididas no tempo com três repetições. Testaram-se sete espécies de leguminosas, Mucuna aterrima, Canavalia ensiformis, Cajanus cajan, Dolichos lab-lab, Crotalaria juncea, Crotalaria spectabilis e Vigna unguiculata, uma mistura das leguminosas mais milho, sorgo e girassol, e a vegetação espontânea, como testemunha. Determinaram-se, para cada tratamento, a quantidade de massa verde, massa seca e teores de N, P, K, Na, Ca e Mg da parte aérea, a partir dos quais foi calculada a quantidade de nutrientes acumulada por hectare. As quantidades de matéria fresca e seca da parte aérea, altura de plantas e número de folhas do sorgo semeado após a incorporação dos adubos verdes, foram avaliadas aos 20, 40, 60, 80 e 100 dias após o plantio. A Mucuna aterrima mostrou-se a melhor opção como adubo verde em virtude de proporcionar os maiores valores das características avaliadas ao final do ciclo do sorgo podendo-se, também, recomendar a Crotalaria juncea e o Dolichos lab-lab, o qual apresentou a maior massa seca e quantidade de nutrientes na parte aérea.The effect of green manure on growth and yield of forage sorghum BR 601 was studied in a trial carried out at the UFERSA, Mossoró, RN, Brazil. The experimental design was completely randomized blocks in a scheme of split plots in time, with three replications. Treatments tested were seven legume species, Mucuna aterrima, Canavalia ensiformis, Cajanus cajan, Dolichos lab-lab, Crotalaria juncea, Crotalaria spectabilis, and Vigna unguiculata, a mixture of the legumes plus corn, sorghum and sunflower, and spontaneous vegetation as a control. For each treatment the amount of fresh mass, dry mass and contents of N, P, K, Na, Ca and Mg in

  4. Fluxo difusivo de micronutrientes catiônicos afetado pelo tipo, dose e época de incorporação de adubos verdes ao solo Diffusive flux of cationic micronutrients as affected by type, dose and timing of green manure incorporation into soil

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    Rodinei Facco Pegoraro

    2006-12-01

    Full Text Available Os solos brasileiros, principalmente os do cerrado, são bastante intemperizados e pobres em alguns micronutrientes catiônicos na solução do solo. A utilização de técnicas de manejo, como a adubação verde, pode favorecer o fluxo difusivo (FD e a disponibilidade desses nutrientes para as plantas. O presente trabalho visou avaliar se a incorporação de adubos verdes ao solo, em diferentes doses e épocas, modifica o FD e a forma iônica de transporte dos micronutrientes Zn, Cu, Fe e Mn no solo. Para tanto, foram incorporados dois resíduos vegetais largamente cultivados como adubo verde: o feijão guandu (Cajanus cajan ou o milheto (Pennisetum americanum por diferentes períodos (0, 15, 25, 35, 45 e 55 dias e doses (0, 9, 18 e 36 t ha-1 num Latossolo Vermelho, argiloso, em condições de laboratório. Para avaliar o FD, utilizaram-se resinas de troca aniônica (positivamente carregada e de troca catiônica (negativamente carregada na forma de lâmina, incubadas junto ao solo em câmaras de difusão durante 15 dias. Os resultados obtidos demonstraram que houve aumento do FD do Cu e do Fe com o aumento das doses de material vegetal, principalmente no início do período de incubação, e maior fluxo desses dois micronutrientes para a resina aniônica em relação à catiônica, possivelmente por ser o seu transporte no solo mais dependente da formação de complexos organometálicos com carga líquida negativa. Já para Zn e Mn, o fluxo difusivo foi maior para a resina catiônica. O aumento do tempo de incubação favoreceu o fluxo difusivo de Mn e Zn e reduziu o do Cu e Fe.Brazilian soils, especially in the Cerrado region, are highly weathered and poor in some cationic micronutrients in the soil solution. Diffusion is the main transport mechanism to the root surface. The adoption of management practices such as the use of green manures might favor the diffusive flux (DF and availability of micronutrients to plants. The present experiment

  5. Caracterização de isolados de Rhizoctonia associados à queima foliar em Roraima = Characterization of Rhizoctonia isolates associated with foliar blight in Roraima.

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    Dayane Rodrigues Youssef

    2012-08-01

    Full Text Available O objetivo desse trabalho foi caracterizar isolados do fungo Rhizoctonia associados à queima foliar, obtidos de hospedeiros de importância econômica no estado de Roraima. Os isolados foram obtidos de plantas de feijão-caupi (Vigna unguiculata, soja (Glycine max, seringueira (Hevea brasiliensis, melancia (Citrullus lanatus, alface (Lactuca sativa e feijão-guandu (Cajanus cajan. Os parâmetros utilizados foram números de núcleos, grupo de anastomose e as características culturais da colônia, taxa de crescimento micelial e a formação de escleródios nos meios de cultura: batata dextrose agar (BDA, BDA+asparagina, BDA+extrato de levedura, Czapek Agar, maltose-peptona-agar, soil extract agar, sacarose-yeast-asparagina e V-8. Todos os 10 isolados estudados foram caracterizados como multinucleados e pertencentes à espécie Rhizoctonia solani. Três isolados de feijão-caupi, um de soja e o isolado de melancia foram identificados como AGI-1A e um isolado de feijãocaupi, um de soja e o isolado de feijão-guandu como AGI-1B. O isolado de seringueira não foi identificado como nenhum dos padrões de anastomose utilizado. Para a maioria dos isolados as maiores taxas de crescimento micelialforam obtidas no meio de cultura Soil Extract Agar. Dois tipos de escleródios, característicos do grupo AGI, foram observados: formação de 2-20 tufos placa-1 coloração variável, 1-2 mm e formação de 38-611 microescleródios placa-1, de coloração marrom, medindo 100 μm. A produção e o tipo de escleródio variaram com o isolado e o meio de cultura utilizado.The aim of this work was to characterize Rhizoctonia isolates associated with foliar blight symptom from hosts with economic importance at Roraima state. The isolates were recovered from cowpea (Vigna unguiculata, soybean (Glycine max, rubber tree (Hevea brasiliensis, watermelon (Citrullus lanatus, lettuce (Lactuca sativa and pigeonpea (Cajanus cajan. The evaluated characteristics were nuclear

  6. Avaliação de um sistema de cultivo em aléias em um argissolo franco-arenoso da região amazônica Evaluation of an alley cropping system under humid tropical conditions of the amazon region

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    Emanoel Gomes de Moura

    2008-08-01

    Full Text Available No trópico úmido, a construção e manutenção da fertilidade dos solos são os maiores desafios dos que se dedicam à implantação de sistemas agrícolas sustentáveis. O objetivo deste estudo foi avaliar um sistema de cultivo em aléias com guandu, associado à adição anual de calcário e de K, em um Argissolo de textura franco-arenosa, a fim de verificar a possibilidade do uso desse sistema como alternativa ao corte e queima na agricultura do trópico úmido. Foram utilizados, como leguminosa, o guandu (Cajanus cajan e a cultura do milho. Os tratamentos foram os seguintes: T = testemunha, com solo desnudo; G2, G2,5 e G3, tratamentos com fileiras de guandu nos espaçamentos de 2, 2,5 e 3 m, respectivamente; G2K, G2,5K e G3K, tratamentos com guandu nos mesmos espaçamentos mais K; G2C, G2,5C e G3C, tratamentos com guandu mais calagem; G2KC, G2,5KC e G3KC, tratamentos com guandu mais K e calagem. A cobertura e o equilíbrio de nutrientes do solo foram os mais importantes fatores que influenciaram a produtividade do milho no sistema de cultivo em aléias com guandu; portanto, eles devem ser considerados como fundamentais para o manejo sustentável dos Argissolos de textura franco-argilosa do trópico úmido.In the humid tropics the buildup and maintenance of soil fertility are major challenges in terms of sustainability of agroecosystems. The objective of this study was to evaluate an alley cropping system with pigeon pea (Cajanus cajan on an Alfisol, with annual application of lime and potassium, with special concern regarding the viability of this system as an alternative to slash and burn practiced in tropical agriculture. Pigeon pea and maize (Zea mays were planted in the experiment with the following treatments: T = control, with bare soil; G2, G2.5 and G3 = treatments with pigeon pea, spacing between lines of 2, 2.5 and 3 m, respectively; G2K, G2.5K and G3K = pigeon pea, same spacing, plus potassium; G2C, G2.5C and G3C = pigeon pea

  7. Avaliação de espécies leguminosas na formação de cafezais no segmento da agricultura familiar no Acre Evaluation of legume species for coffee plants formation in the segment of family farms in Acre

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    Celso Luis Bergo

    2006-03-01

    Full Text Available Leguminosas quando consorciadas com o café e usadas como adubação verde podem contribuir fornecendo nitrogênio e proteção ao solo pela adição de matéria orgânica. O trabalho foi conduzido com o objetivo de avaliar o efeito do uso de leguminosas no sistema de produção de café, no segmento de agricultura familiar, visando promover a implantação e a manutenção dos cafezais de forma técnica e economicamente sustentável. O experimento foi conduzido no período de novembro de 2000 a abril de 2003, em delineamento experimental de blocos ao acaso em esquema de parcelas subdivididas, com quatro repetições. Os tratamentos utilizados nas parcelas foram as leguminosas (Stizolobium atterrimum, Cajanus cajan, Canavalia endiformis e Flemingia congesta e a testemunha (sem leguminosa e, nas subparcelas, duas doses de N (0 e 22 g de N por cova. A Flemingia congesta e a Mucuna aterrima foram as leguminosas que mais influenciaram positivamente a produtividade dos cafeeiros, independente da adubação nitrogenada. Em relação à testemunha, o aumento em produção foi de 109% quando utilizou-se a Flemingia congesta e 52% com a Mucuna aterrima. A Flemingia congesta foi também a leguminosa que melhor controlou as invasoras, dado o volume de fitomassa produzida e a possibilidade de 2 cortes durante um período de doze meses, evidenciando o potencial desta leguminosa na formação de novos cafezais no Acre. Por outro lado, a Canavalia ensiformis, leguminosa que é normalmente utilizada nas entrelinhas dos cafeeiros pelos cafeicultores, do Acre e de outras regiões produtoras, neste trabalho influenciou negativamente a altura das plantas, diâmetro da copa e crescimento dos cafeeiros.Legumes, when associated with coffee crop and used as green manure can contribute with nitrogen and protect the soil by addition of organic matter. The effect of the use legume species in the coffee production system of family farms, was evaluated in order to promote

  8. Níveis de farelo de melancia forrageira em dietas para ovinos Levels of forage watermelon meal in diets for sheep

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    Raimundo Luiz Nunes Vaz da Silva

    2009-06-01

    Full Text Available Com o objetivo de estudar a inclusão de 20, 35, 50, 65 ou 80% de farelo de melancia forrageira (Citrullus lanatus cv. citroides (FMF em dietas à base de feno de guandu (Cajanus cajan, foi realizado um ensaio de digestibilidade utilizando-se 20 cordeiros com 6 meses de idade e peso vivo de 11,2 ± 1,2 kg. O experimento constou de 20 dias de adaptação e 5 de coleta total de sobras e de fezes. O farelo de melancia forrageira e o feno de guandu apresentaram 95,3 e 95,75% de matéria seca (MS, 18,7 e 16,8% de proteína bruta (PB e 38,8 e 64,3% de fibra em detergente neutro (FDN. Os níveis de farelo de melancia forrageira determinaram aumento quadrático nos consumos de MS, MO, PB, FDN, FDA, hemicelulose e carboidratos totais. O consumo de extrato etéreo não foi alterado, mas o consumo de CNF teve aumento linear conforme aumentaram os níveis de farelo de melancia forrageira. Os coeficientes de digestibilidade de MS (58%, PB (69%, FDN (41% e EE (85% não foram influenciados pelo nível de farelo de melancia forrageira. As digestibilidades de MO (54 a 62%, FDA (36 a 49% e carboidratos totais (47 a 58% variaram linear e positivamente, enquanto a digestibilidade da hemicelulose teve comportamento inverso. As dietas proporcionaram consumo de matéria seca que atendeu a exigência dos animais. Os coeficientes de digestibilidade observados foram satisfatórios. O farelo de melancia forrageira pode ser indicado para compor dietas à base de feno de guandu em níveis de 35 a 66% da matéria seca.In order to study the inclusion of forage watermelon (Citrullus lanatus var. Citroides meal (FWM in pigeon pea hay based diets (Cajanus cajan (PPH, a digestibility trial in sheep was carried out for testing five levels of FWM (20, 35, 50, 65 and 80%. Twenty lambs with an average age of six months and live weight of 11.2 kg (± 1.2 were used. The experimental period was twenty days for adaptation and five days for total refuses and feces collection. The chemical

  9. Sistemas de cultivo, sucessões de culturas, densidade do solo e sobrevivência de patógenos de solo Cropping systems and previous crops on soil density and survival of soil-borne pathogens

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    Eliane Divina de Toledo-Souza

    2008-08-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito de sistemas de manejo do solo e de cultivos prévios ao plantio do feijoeiro (Phaseolus vulgaris L. sobre a densidade do solo e as populações de Rhizoctonia spp. e de Fusarium spp. Os cultivos prévios incluíram as leguminosas: guandu-anão (Cajanus cajan, estilosantes (Stylosanthes guianensis cv. Mineirão e crotalária (Crotalaria spectabilis; e as gramíneas: milheto (Pennisetum glaucum cv. BN-2, sorgo granífero (Sorghum bicolor cv. BR 304, capim-mombaça (Panicum maximum cv. Mombaça, braquiária (Brachiaria brizantha cv. Marandu e milho (Zea mays consorciado com braquiária. As culturas utilizadas no cultivo prévio foram semeadas nos verões de 2002, 2003 e 2004, e os plantios de feijoeiro, cultivar BRS Valente, foram realizados nos invernos subseqüentes de 2003, 2004 e 2005, com irrigação por pivô central. Os restos culturais dos cultivos eram incorporados ao solo, no plantio convencional, e ficavam à superfície, no plantio direto. De modo geral, as maiores populações de Fusarium spp. e Rhizoctonia spp. e as maiores densidades de solo foram encontradas no solo cultivado em plantio direto. As maiores populações de Rhizoctonia spp. foram observadas em solos mais adensados. As leguminosas geralmente aumentaram populações desses patógenos e devem ser evitadas como culturas prévias ao cultivo do feijoeiro, em ambos os sistemas de cultivo. Plantios prévios de gramíneas, em geral, são supressores das populações de Rhizoctonia spp. e de Fusarium spp. no solo.The objective of this work was to evaluate the effect of cropping systems and crops cultivated previously to common beans (Phaseolus vulgaris L. on soil density and soil populations of Rhizoctonia spp. and Fusarium spp. Previous crops included the following legumes: Cajanus cajan,Stylosanthes guianensis cv. Mineirão and Crotalaria spectabilis; and the following grasses: Pennisetum glaucum (cv. BN-2, millet, Sorghum bicolor

  10. Supressão de plantas daninhas e produção de milho-verde orgânico em sistema de plantio direto Weed suppression and organic green corn production in no tillage system

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    L.R. Queiroz

    2010-06-01

    Full Text Available O sombreamento do solo com cobertura morta proporciona redução na germinação das sementes e diminuição da população de plantas daninhas, possibilitando às plantas da cultura de interesse se desenvolverem sob efeito de menor competição inicial. Dessa forma, objetivou-se neste trabalho investigar o efeito do cultivo de leguminosas na evolução da comunidade de plantas daninhas na cultura do milho-verde cultivado em sucessão, num sistema orgânico. O ensaio foi realizado em delineamento em blocos ao acaso, com parcelas subdivididas e quatro repetições. Inicialmente, houve o plantio das leguminosas: feijão-de-porco (Canavalia ensiformes, guandu (Cajanus cajan, mucuna-preta (Mucuna aterrinum, mucuna-anã (Mucuna deeringiana e crotalária (Crotalaria juncea; foi mantida uma testemunha sem cultivo em pousio. Anteriormente ao cultivo do milho, foi avaliada a produção de matéria seca de cada espécie de leguminosa. Em seguida, após a roçada das leguminosas foi semeado sobre a palhada o milho, cultivar HTMV 02. A amostragem das plantas daninhas foi realizada aos 15 e 30 dias após a emergência do milho, lançando-se de forma aleatória sobre cada parcela um quadro de 50 x 50 cm. As plantas daninhas dentro do quadro foram identificadas, pesadas e contadas por espécie, sendo posteriormente colocadas em estufa a 65ºC, por 72 horas, para determinação da matéria seca. As palhadas da mucuna-preta e da crotalária proporcionaram maior redução de matéria seca e população das plantas daninhas. A maior produtividade de espigas comerciais de milho-verde foi obtida na área de palhada de mucuna-preta e crotalária.Soil shading using mulch reduces weed seed germination and, consequently, weed population, allowing the plant of interest to develop free of the initial competition. Thus, the aim of this paper was to evaluate the effect of leguminous plant cultivation on the evolution of a weed community on green corn cultivated in

  11. Restos vegetais e adubação nitrogenada na micorrização e produtividade do arroz de terras altas em sistema plantio direto = Plant residues and nitrogen fertilization on the mycorrhization and upland rice yield on no tillage system

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    Tânia Oshiro Nosse

    2008-10-01

    Full Text Available O objetivo deste trabalho foi verificar a influência de restos vegetais e da adubação nitrogenada em cobertura na micorrização (colonização, número de esporos dos fungos micorrízicos arbusculares e produtividade de grãos de arroz de terras altas em plantio direto. Oexperimento foi conduzido na Fazenda Experimental da Unesp/Campus de Ilha Solteira, município de Selvíria (Estado do Mato Grosso do Sul. Nas parcelas principais foram testadas as leguminosas: crotalária (Crotalaria juncea L., guandu (Cajanus cajan L. e a mucuna-preta(Stizolobium aterrimum Piper & Tracy e as gramíneas: milho (Zea mays L., milheto (Pennisetum americanum L. e sorgo-granífero (Sorghum bicolor L., mais o pousio (vegetação espontânea, como cobertura de solo. Nas subparcelas, após a semeadura do arroz, foi utilizada adubação nitrogenada em cobertura (zero e 75 kg ha-1 de N, na forma de uréia. A produtividade de grãos do arroz foi influenciada pelos restos vegetais, mas não pelas doses ou pela interação. O sorgo mostrou influência negativa sobre a produtividade do arroz. A adubação nitrogenada influenciou a esporulação, mas não a colonização micorrízica ou a produtividade de grãos, na época da colheita.Não houve correlação entre as variáveis estudadas.The objective of this research was to verify the influence of plant residues and the nitrogen fertilization in covering on the mycorrhization (colonization and number of spores of the arbuscular mycorrhizal fungi, and grain yield of upland rice grown under a no tillage system. The experiment was conducted at the Experimental Farm Station of Unesp/Ilha Solteira Campus, at Selvíria (Mato Grosso do SulState. In the main plots, the leguminous were tested: sun hemp (Crotalaria juncea L., pigeon pea (Cajanus cajan L. and velvet bean (Stizolobium aterrimum Piper & Tracy and the grass: corn (Zea mays L., millet (Pennisetum americanum L. and grain sorghum (Sorghumbicolor L., plus no planted area

  12. Microclimatic characterization and productivity of coffee plants grown under shade of pigeon pea in Southern Brazil Caracterização microclimática e produtividade de cafeeiros sombreados com guandu no Sul do Brasil

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    Heverly Morais

    2006-05-01

    Full Text Available Recent studies on coffee (Coffea arabica L. cultivation in agroforestry systems in Southern Brazil have shown the potential of partial shading to improve management of this crop. The objective of this work was to evaluate microclimatic conditions and their effects on coffee production of plants shaded with pigeon pea (Cajanus cajan in comparison to unshaded ones, from May 2001 to August 2002 in Londrina, State of Paraná, Brazil. The appraised microclimatic characteristics were: global radiation, photosynthetic and radiation balance; air, leaf and soil temperatures; and soil humidity. Shading caused significant reduction in incident global solar radiation, photosynthetically active radiation and net radiation, and attenuated maximum leaf, air and soil temperatures, during the day. Shade also reduced the rate of cooling of night air and leaf temperatures, especially during nights with radiative frost. Soil moisture at 0-10 cm depth was higher under shade. The shaded coffee plants produced larger cherries due to slower maturation, resulting in larger bean size. Nevertheless, plants under shade emitted less plagiotropic branches, with smaller number of nodes per branch, and fewer nodes with fruits, resulting in a large reduction in coffee production. These results show the need to find an optimal tree density and management that do not compromise coffee production and protect against extreme temperatures.Recentes estudos sobre cafeeiros (Coffea arabica L. cultivados em sistemas agroflorestais no Sul do Brasil têm mostrado o potencial do sombreamento parcial no manejo desta cultura. O objectivo deste trabalho foi avaliar as condições do microclima e seus efeitos na produção de café sombreado com guandu (Cajanus cajan, em comparação ao cultivado a pleno sol, no período de maio de 2001 a agosto de 2002 em Londrina, PR. As características microclimáticas avaliadas foram: radiação global, fotossintética e saldo de radiação; temperaturas

  13. Digestibilidade dos nutrientes de alimentos volumosos determinada pela técnica dos sacos móveis em eqüinos Nutrient digestibility of forage feed determined using mobile bag technique in horses

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    Vinícius Pimentel Silva

    2009-01-01

    Full Text Available Objetivou-se estimar a digestibilidade de nutrientes de forrageiras em eqüinos utilizando-se a técnica de sacos de náilon móveis. Foram avaliados alfafa (Medicago sativa, amendoim forrageiro (Arachis pintoi, desmódio (Desmodium ovalifolium, estilosantes (Stylosanthes guianensis, guandu (Cajanus cajan, macrotiloma (Macrotyloma axillare e capim-coastcross (Cynodon dactylon cv. coastcross. O delineamento foi em blocos inteiramente casualizados com sete alimentos e cinco blocos (animais. Foram utilizados cinco eqüinos mestiços com 17 a 27 anos de idade e peso vivo médio de 350 kg. O ensaio teve duração de 12 dias: três para a adaptação às baias, cinco para inserção gástrica dos sacos através de sonda nasogástrica e quatro de coleta dos sacos nas fezes. No período pré-experimental de 30 dias, os animais foram mantidos em piquetes com dieta composta de 80% de feno de coastcross e 20% de concentrado. Na confecção dos sacos, utilizou-se náilon com porosidade de 45 µ e dimensão de 7,5 × 2 cm. Em cada saco, foram inseridos 510 mg de matéria seca de amostra do alimento. Os coeficientes de digestibilidade dos nutrientes das forragens foram calculados considerando o resíduo obtido no saco. A digestibilidade dos nutrientes do amendoim, estilosantes e macrotiloma foram superiores à da demais forrageiras, com destaque para a digestibilidade da proteína bruta, cujos valores foram de 91,4; 94,9 e 97,0%, respectivamente. O amendoim e macrotiloma apresentaram digestibilidade da fibra em detergente neutro de 72,3 e 65,2% e da fibra em detergente ácido de 70,9 e 59,4%, respectivamente. O amendoim forrageiro, macrotiloma e estilosantes apresentam digestibilidade dos nutrientes satisfatória e têm potencial para o uso em dietas para eqüinos.This work was carried out to evaluate the nutrient digestibility of forages using mobile bags technique in horses. The forages were lucerne (Medicago sativa, peanut (Arachis pintoi, desmodio

  14. Carbon and nitrogen stocks under different management conditions of a yellow-red ultisol, cultivated with sweet corn in Sergipe coastal tablelands Estoques de carbono e nitrogênio sob diferentes condições de manejo de um Argissolo Vermelho Amarelo, cultivado com milho doce nos tabuleiros costeiros de Sergipe

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    Wellma Nascimento Pedra

    2012-12-01

    Full Text Available This study was carried out in a long term experiment and aimed to evaluate the effects of different tillage systems associated with cover crops following sweet corn (Zea mays L. cultivation on soil C and N stocks. The soil was a red-yellow Ultisol, characteristic of the coastal tableland in the estate of Sergipe. Experimental bands over a split plot scheme were set up with 12 treatments: three management systems (conventional tillage (CC, reduced tillage (CM and, No-tillage (PD, in the plots, and four cover crops (peanut (Arachis hypogeae, common bean (Phaseolus vulgaris, pigeon pea (Cajanus cajan and, crotalaria (Crotalaria juncea, in the subplots, with three replications. The highest soil organic carbon input was observed on the No-tillage system as compared to the other tillage systems. As far as N content is concerned, both No-tillage and reduced tillage systems contributed the most. Notillage system associated with cover crops such as Common bean, Peanut and Pigeon pea contributed to a higher soil organic carbon stock while higher N stock was observed when Pigeon pea was associated with reduced tillage system. No-tillage and reduced tillage systems presented higher C and N stocks, respectively, when compared to conventional tillage, improving agriculture sustainability. O objetivo deste estudo foi analisar o efeito de diferentes sistemas de cultivo com plantas de cobertura em sucessão ao milho doce (Zea mays L. no estoque de C e N do solo, em experimento de longa duração conduzido em um Argissolo Vermelho-Amarelo nos Tabuleiros Costeiros no Estado de Sergipe. Utilizou-se um sistema de faixas experimentais sob parcelas subdivididas, compostos por 12 tratamentos que associam três manejos de solos (parcelas, compostas por sistema de cultivo convencional (CC, cultivo mínimo (CM e o sistema plantio direto (PD e, como subparcelas, quatro plantas, sendo duas comerciais [amendoim (Arachis hypogeae, feijão (Phaseolus vulgaris e duas de

  15. Digestão dos carboidratos de alimentos volumosos em eqüinos Digestion of carbohydrates of forages in horses

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    Eliane da Silva Morgado

    2009-01-01

    Full Text Available Neste estudo, foram realizados dois ensaios com os objetivos de avaliar o fracionamento dos carboidratos de alimentos volumosos e estimar a digestibilidade desses nutrientes em dois ensaios de digestão com eqüinos. No ensaio 1, foram utilizados cinco eqüinos em delineamento de blocos casualizados, com sete tratamentos - fenos de alfafa (Medicago sativa; amendoim forrageiro (Arachis pintoi; desmódio (Desmodium ovalifolium; guandu (Cajanus cajan; macrotiloma (Macrotyloma axillare; estilosantes (Stylosanthes guianensis; ou coastcross (Cynodon dactylon - avaliados pela técnica de sacos de náilon móveis. No ensaio 2, foram utilizados quatro eqüinos em delineamento quadrado latino 4 × 4, alimentados com feno de coastcross em quatro tipos de moagem com o objetivo de avaliar se a redução do tamanho de partícula interfere na digestibilidade dos carboidratos. Os resultados comprovaram que os eqüinos possuem alta eficiência na digestão dos carboidratos não-fibrosos e de suas frações hidrolisáveis e rapidamente fermentáveis. Os fenos de amendoim forrageiro, estilosantess e macrotiloma apresentaram elevada digestibilidade dos carboidratos fibrosos e não-fibrosos, enquanto a digestibilidade de todos os nutrientes do amendoim forrageiro foi superior a 70%, o que indica potencial para uso desta leguminosa em dietas para eqüinos. O processamento do feno de coastcross não influenciou a digestibilidade das frações dos carboidratos fibrosos e não-fibrosos. A análise dos carboidratos fibrosos e não-fibrosos é um bom indicativo do valor nutricional dos alimentos e pode ser incluída na avaliação da qualidade de alimentos para eqüinos.This work was carried out to evaluate the fractions of carbohydrates and estimate the apparent digestibility of these nutrients in two digestion assays with horses. In assay 1, five horses were allotted to a complete randomized blocks design and treatments were seven forages hays, alfalfa (Medicago sativa

  16. Attraction of Neomegalotomus parvus (Westwood(Heteroptera: alydidae to cow urine and ammonia Atração de Neomegalotomus parvus (Westwood (Heteroptera: alydidae por urina de vaca e amônia

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    Jovenil José da Silva

    2010-02-01

    Full Text Available The alydid bug parvus (Westwood is not easily detected in the field and the discovery of attractants may be a suitable way to monitor this species. The attraction of N. parvus to traps (transparent, transparent green and yellow baited with cow urine and ammonia was studied in two field trials. Traps were placed near a terrace with pigeon pea plants (Cajanus cajan L.. Tap water, NaCl 10% aqueous solution (w/v, cow urine, and ammonia (NH4OH 1% aqueous solution were tested as attractants in yellow, transparent and transparent green traps. Green traps baited with cow urine caught more bugs than yellow and transparent traps (3.2 and 7.2 times more, respectively, and more bugs than traps of the same color with just water (no captures or NH4OH solution (14.5 times more. Traps baited with ammonia caught more bugs than traps with water: 14.4, 4.7 and 6.7 times more than transparent green, yellow, and transparent traps, respectively, or NaCl solution: 3.9, 7.6 and 6.2 times, respectively. Both ammonia and cow urine are attractive to N. parvus and may be tested to monitor the bugs in the field.O percevejo alidídeo Neomegalotomus parvus (Westwood não é facilmente detectado no campo e a descoberta de atraentes pode ser uma forma adequada para monitorar a espécie. A atração de N. parvus a armadilhas (transparentes, verde transparente e amarela iscadas com urina de vaca e amônia foi estudada em dois experimentos em campo. Armadilhas foram colocadas próximas a um terraço com plantas de feijão-guandu (Cajanus cajan L.. Água corrente, solução aquosa de NaCl 10% (p/v, urina de vaca e amônia (solução aquosa de NH4OH 1% foram testadas como atraentes em armadilhas amarelas, transparentes e transparentes verdes foram utilizadas. Armadilhas verdes iscadas com urina capturaram mais insetos do que armadilhas amarelas e transparentes (3,2 e 7,2 vezes mais, respectivamente, e mais insetos do que armadilhas da mesma cor com apenas água (zero insetos ou solu

  17. Fixação biológica e transferência de nitrogênio por leguminosas em pomar orgânico de mangueira e gravioleira Biological fixation and nitrogen transfer by three legume species in mango and soursop organic orchards

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    Gleicia Miranda Paulino

    2009-12-01

    Full Text Available O objetivo deste trabalho foi avaliar a fixação biológica de nitrogênio (FBN e a transferência do N derivado da FBN das espécies leguminosas - gliricídia (Gliricidia sepium, crotalária (Crotalaria juncea e feijão-guandu anão (Cajanus cajan - para um pomar orgânico de mangueira e gravioleira, pelo método da abundância natural de N. Foram avaliados os seguintes sistemas de cultivos consorciados: mangueira e gravioleira com gliricídia; mangueira e gravioleira com crotalária; mangueira e gravioleira com feijão-guandu; e a testemunha mangueira e gravioleira. Agliricídia apresentou maior potencial de FBN (80%, seguida da crotalária (64,5% e feijão-guandu (45%. Em dois cortes, a crotalária forneceu 149,5 kg ha-1 por ano de N, com 96,5kg derivados da FBN. A gliricídia com três podas anuais forneceu 56,4 e 80,3 kg ha-1 por ano de N, com 45 e 64 kg derivados da FBN, em dois anos consecutivos. A quantidade de N fornecida foi superior à demandada pela mangueira e gravioleira. Variações na abundância natural de 15N foram detectadas somente na gravioleira. Gliricídia e crotalária destacaram-se na transferência de N, com cerca de 22,5 e 40% do N fixado, respectivamente. A adubação verde com gliricídia possibilita o parcelamento do N, com melhor aproveitamento pelas espécies frutíferas.The objective of this work was to evaluate the biological nitrogen fixation (BNF and the N transfer derived from BNF of the legume species - Gliricidia sepium (gliricidia, Crotalaria juncea (sunnhemp and Cajanus cajan (pigeon pea - for an intercropped organic orchard with mango and soursop, through the 15N natural abundance method. The following intercropping systems were evaluated: mango and soursop with gliricidia; mango and soursop with sunnhemp; mango and soursop with pigeon pea; and mango and soursop as control. Gliricidia showed the highest BNF potential (80% , followed by sunnhemp (64.5% and pigeon pea (45%. After two sunnhemp prunes, 149

  18. Fitorremediação de solos contaminados com tebuthiuron utilizando-se espécies cultivadas para adubação verde Phytoremediation of tebuthiuron-contaminated soils using species cultivated for green manure

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    F.R. Pires

    2005-12-01

    Full Text Available O emprego da fitorremediação na despoluição de solos contaminados com compostos orgânicos, inclusive herbicidas, vem sendo pesquisado ultimamente. Como o tebuthiuron pode causar sério impacto ambiental, por ser muito utilizado, apresentar longo efeito residual no solo e possibilidade de contaminação do lençol de água subterrâneo, desenvolveu-se este trabalho com o objetivo de avaliar a capacidade de sete espécies vegetais na despoluição de solos contaminados com esse herbicida. As espécies avaliadas neste experimento foram: Cajanus cajan, Canavalia ensiformes, Dolichos lablab, Pannisetum glaucum, Estizolobium deeringianum, Estizolobium aterrimum e Lupinus albus. Elas foram semeadas e cultivadas, por 60 dias, em vasos cujo solo recebeu quatro doses do tebuthiuron (0,0; 0,5; 1,0; e 1,5 kg ha-1. As testemunhas foram constituídas por vasos sem planta, aos quais foram aplicadas as mesmas doses de herbicidas. Aos 60 dias após a semeadura, colheu-se a parte aérea de todas as plantas, sendo semeada, nos mesmos vasos, Avena strigosa, utilizada como planta indicadora, para realização do bioensaio. Depois de 60 dias da semeadura da espécie bioindicadora, esta foi colhida, sendo avaliadas as seguintes características: altura de plantas, sintomas de toxicidade e biomassa seca da parte aérea das plantas. Até a dose de 0,5 kg ha-1 de tebuthiuron, a espécie que melhor fitorremediou esse herbicida no solo foi L. albus. Quando o solo foi tratado com 1,0 kg ha-1 de tebuthiuron, C. ensiformes foi a espécie que melhor fitorremediou o herbicida. Isso foi concluído com base na maior altura de plantas, biomassa seca da parte aérea e menor toxicidade de A. strigosa, quando foi cultivada em sucessão a essas plantas remediadoras. Nenhuma das espécies avaliadas cresceu em solo que recebeu a maior dose de tebuthiuron (1,5 kg ha-1.Phytoremediation of soil contaminated by organic compounds, including herbicides, is being widely investigated. The

  19. Comportamento de dois genótipos de milho cultivados em sistema de aléias preestabelecido com diferentes leguminosas arbóreas Behaviour of two maize genotypes grown in alley cropping system pre-established with diferents leguminous trees

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    Andréia Araújo Lima Leite

    2008-12-01

    Full Text Available O cultivo em aléias tem sido recomendado como alternativa para a substituição da agricultura de corte e queima, no trópico úmido, devido à grande capacidade de produção de matéria orgânica e de reciclagem de nutrientes, mas algumas dúvidas quanto à sustentabilidade e à competição interespecífica são persistentes. O objetivo no trabalho foi avaliar a viabilidade da cultura do milho em um sistema de cultivo em aléias de leguminosas arbóreas. O delineamento experimental utilizado foi em blocos casualisados, com quatro repetições dos tratamentos: aléias de sombreiro (Clitoria fairchildiana, ingá (Inga edulis, guandu (Cajanus cajan e leucena (Leucaena leucocephala e uma testemunha sem aléias. Foram avaliadas a remobilização de carbono e nitrogênio, massa de grãos, massa de mil grãos e competição interespecífica entre as cultivares de milho e as leguminosas. A produção de grãos foi maior nas parcelas com C. fairchildiana e L. leucocephala. A produtividade do híbrido de milho foi superior à da variedade em todos os tratamentos. A produtividade e a massa de mil grãos de milho não são negativamente afetadas pela distância da linha da leguminosa arbórea. Esse estudo conclui que o sistema de aléias com leguminosas arbóreas é uma alternativa importante ao manejo sustentável dos agroecossistemas no tropico úmido. Além disso, nessa região a produtividade em grãos na cultura do milho é favorecida no sistema de aléias preeestabelecidas com as leguminosas arbóreas sombreiro, ingá e leucena e pela utilização de genótipos eficientes no aproveitamento do nitrogênio, cujo sincronismo entre a liberação e a absorção do N aplicado por meio das leguminosas deve ser aprimorado.Alley cropping has been recommended as alternative land use to slash-and-burn agriculture in humid tropics. However, interespecific competition between cash crop and hedgerow can reduce this potential. This study aimed to evaluate the

  20. Efeito de coberturas mortas vegetais sobre o desempenho da cenoura em cultivo orgânico Effect of mulch of different plant species on the performance of organically grown carrot

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    CAB Santos

    2011-03-01

    Full Text Available Avaliou-se o efeito de coberturas mortas com resíduos de diferentes espécies de plantas no rendimento da cenoura em cultivo orgânico e nos níveis de reinfestação pela vegetação espontânea. O experimento foi conduzido no Sistema Integrado de Produção Agroecológica ("Fazendinha Agroecológica Km 47", em Seropédica-RJ, empregando delineamento de blocos casualizados, com quatro tratamentos e seis repetições. Os tratamentos consistiram de coberturas dos canteiros com a biomassa aérea, seca e triturada, de capim Cameroon (Pennisetum purpureum, gliricídia (Gliricidia sepium e guandu (Cajanus cajan, além da testemunha (sem qualquer cobertura. Por ocasião da colheita da cenoura, determinou-se a produtividade e as médias em peso, comprimento e diâmetro máximo das raízes tuberosas. A reinfestação de ervas espontâneas foi estimada pelo número total de indivíduos por m², sendo as coletas realizadas aos 20, 50 e 80 dias após a semeadura. A cenoura cultivada nas parcelas tratadas com resíduos de leguminosas apresentou aumentos significativos de todas as variáveis fitotécnicas, com exceção para o comprimento médio da raiz. Nas parcelas cobertas com resíduos da gramínea, os resultados foram estatisticamente iguais aos da testemunha. A reinfestação por plantas espontâneas por unidade de área cultivada alcançou níveis da ordem de 300% superiores em parcelas sem cobertura do solo, na comparação com aquelas que receberam as palhadas.The effect of soil mulch with different plant species was evaluated in relation to the performance of organically grown carrots and to weed population levels. The experiment was carried out at the Integrated Agroecological Production System located in Seropédica, Metropolitan Region of Rio de Janeiro State (Baixada Fluminense, Brazil, using a randomized blocks design comprising four treatments replicated six times. These treatments consisted of soil mulch with chopped and dried above

  1. Dinâmica do potássio nos resíduos vegetais de plantas de cobertura no Cerrado Potassium dynamics in crop residues of cover plants in Cerrado

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    José Luiz Rodrigues Torres

    2008-08-01

    Full Text Available A produção de biomassa, a manutenção dos resíduos vegetais sobre o solo e sua posterior decomposição são fatores de grande importância no estudo da ciclagem de nutrientes. Este estudo foi desenvolvido na área experimental do CEFET-Uberaba-MG, onde foram avaliados oito tipos de coberturas vegetais: milheto (Pennisetum americanum sin. tiphoydes, braquiária (Brachiaria brizantha, sorgo-forrageiro (Sorghum bicolor L. Moench, guandu (Cajanus cajan (L. Mill sp., crotalária (Crotalarea juncea, aveia-preta (Avena strigosa Schreb, pousio e área em preparo convencional de solo (testemunha em área de Cerrado, na região do Triângulo Mineiro. Avaliaram-se a fitomassa seca (FS, a decomposição dos resíduos em bolsas de decomposição, e a liberação de K. Utilizou-se um modelo matemático para descrever a decomposição dos resíduos e a liberação de K, e calcularam-se a constante de decomposição (k e o tempo de meia-vida (T½. O milheto, o sorgo e a crotalária foram as coberturas que apresentaram maiores produções de matéria seca. O maior acúmulo de K ocorreu em gramíneas e a maior liberação de K ocorreu no milheto, aveia, braquiária e crotalária nos primeiros 42 dias após manejo, nos dois períodos avaliados. A braquiária apresentou o menor T½ vida e a maior taxa de liberação de K.Crop residue production, plant residue maintenance and their decomposition are important factors in the understanding of nutrient recycling process. To evaluate K accumulation and release a study with eight cover crops types was developed: pearl millet (Pennisetum americanum sin. tiphoydes, brachiaria grass (Brachiaria brizantha, sorghum (Sorghum bicolor L. Moench, pigeonpea (Cajanus cajan (L. Millsp, sunn hemp (Crotalarea juncea and black oats (Avena strigosa Schreb, fallow land and conventional culture (control in the experimental area of CEFET-Uberaba-MG, in a Cerrado area. The dry mass production, crop residue decomposition in litter bags

  2. Qualidade fisiológica de sementes de feijão em plantio direto sobre diferentes coberturas de plantas em Diamantina, MG Physiological quality seeds of bean in no-tillage system over different coverings of plants in Diamantina, MG

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    Ubirajara Russi Nunes

    2007-12-01

    Full Text Available Objetivou-se com este trabalho avaliar o efeito de diferentes coberturas de plantas sobre a qualidade fisiológica de sementes de feijão em plantio direto no município de Diamantina - MG. O experimento foi instalado no Campus II da UFVJM, num Neossolo Quartzarênico Órtico típico, no delineamento em blocos casualizados, com dez tratamentos e quatro repetições. Os tratamentos constituíram-se de palhada dessecada e roçada de 1 Brachiaria decumbens cv. Basilisk, 2 Brachiaria brizantha cv. Marandu, 3 Panicum maximum cv. Tanzânia, 4 Panicum maximum cv. Mombaça, 5 Mucuna aterrina (mucuna preta, 6 Calopogonio muconoides cv. Calopogonio, 7 Dolichos lab lab (lab lab, 8 Cajanus cajan (guandu, 9 Cobertura natural e 10 Plantio convencional onde se efetuou o plantio de feijão cultivar Talismã. A qualidade fisiológica das sementes foi avaliada pelos testes de germinação (primeira contagem e contagem final; condutividade elétrica dos solutos; peso de 100 sementes; comprimento e peso das plântulas; índice de velocidade de germinação e teor de água das sementes de feijão. As gramíneas Panicum. maximum cv. Mombaça, Brachiaria brizantha cv. Marandu, B. decumbens cv. Basilisk e P. maximum cv. Tanzânia produziram matéria seca em quantidade e uniformidade para maior conservação de água e proteção do solo para viabilizar o sistema plantio direto. A qualidade das sementes não foi afetada pelos manejos de solo utilizados e sim pelas condições climáticas na maturação.The objective this work was evaluate the effect of different coverings plants on the physiological quality seeds of bean in no-tillage system in Diamantina - MG. The experiment was installed in the Campus II of UFVJM, in a Neossolo Quartzarênico Órtico típico, in blocks randomized design, with ten treatments and four replicates. The treatments were constituted of control chemical and rubbed straw of 1 Brachiaria decumbens cv. Basilisk, 2 Brachiaria brizantha cv

  3. Produção de palhada de plantas de cobertura e rendimento do feijão em plantio direto Covering crops straw production and common bean productivity in no-tillage system

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    Ubirajara Russi Nunes

    2006-06-01

    Full Text Available O objetivo deste trabalho foi avaliar a produção de palhada e o efeito de diferentes plantas de cobertura sobre características do feijão, em sistema de plantio direto, em Diamantina, MG. O experimento foi instalado num Neossolo Quartzarênico Órtico típico, utilizando-se o delineamento em blocos ao acaso e três repetições. Os tratamentos constituíram-se de: Brachiaria decumbens, B. brizantha, Panicum maximum cv. Tanzânia, P. maximum cv. Mombaça, Mucuna aterrima, Calopogonio mucunoides cv. Calopogônio, Dolichos lab lab, Cajanus cajan, Crotalaria juncea, pousio e testemunha, sobre os quais foi cultivado feijão cv. Talismã. Avaliou-se a massa de matéria seca das plantas de cobertura e, no feijoeiro, estande de plantas, número de sementes, número de vagens, número de sementes por vagem, altura de plantas, peso de 100 sementes e produtividade. Não houve diferença significativa entre os tratamentos para as variáveis número de sementes, número de vagens, número de sementes por vagem e altura das plantas. Os maiores valores de peso de 100 sementes e de produtividade de feijão foram obtidos nas parcelas com as gramíneas P. maximum cv. Mombaça, B. brizantha, B. decumbens e P. maximum cv. Tanzânia, que também produziram quantidade suficiente de matéria seca para viabilizar o sistema de plantio direto de feijão.The objective of this work was to evaluate the straw production and the effect of different covering plants on some common bean characteristics, in no-tillage system, in Diamantina, MG, Brazil. A radomized block was used as experimental design, with three replications. Treatments were constituted of: Brachiaria decumbens, B. brizantha, Panicum maximum cv. Tanzania, P. maximum cv. Mombaça, Mucuna aterrima, Calopogonio mucunoides cv. Calopogônio, Dolichos lab lab, Cajanus cajan, Crotalaria juncea, fallow and control, on which common bean cultivar Talismã was planted. For covering, the weight of dry matter was

  4. Coberturas vegetais, doses de nitrogênio e inoculação de sementes com Azospirillum brasilense em arroz de terras altas no sistema plantio direto Cover crops, nitrogen rates and seeds inoculation with Azospirillum brasilense in upland rice under no-tillage

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    Douglas de Castilho Gitti

    2013-01-01

    Full Text Available O arroz é importante fonte de energia e proteínas para a população mundial, principalmente na Ásia e Oceania. No Brasil, juntamente com o feijão, constitui a base da alimentação. Tecnologias sustentáveis que reduzam custos da produção e aumentem a produtividade do arroz podem garantir seu suprimento em períodos de alta demanda. O objetivo deste trabalho foi avaliar diferentes coberturas vegetais antecessoras (milheto [Pennisetum americanum], crotalária [Crotalaria juncea], guandu [Cajanus cajan], braquiária [Brachiaria ruziziensis], milheto + crotalária e milheto + guandu, doses de nitrogênio (N em cobertura (0, 40, 80 e 120 kg ha-1 e o efeito da inoculação de sementes com Azospirillum brasilense no arroz de terras altas em sistema plantio direto no desenvolvimento e na produtividade. Utilizou-se o delineamento experimental em blocos casualizados em esquema fatorial 6x4x2 com quatro repetições. O estudo foi desenvolvido em um Latossolo Vermelho, em Selvíria, Estado do Mato Grosso do Sul, Brasil, em 2011/2012. O cultivo do guandu antecedendo o arroz proporcionou maior produtividade do arroz somente em comparação a B. ruziziensi. A produtividade do arroz em função das doses de N em cobertura se ajustou a uma função quadrática. Não houve influência da inoculação de sementes com A. brasilense sobre a produtividade do arroz, porém houve interação entre a inoculação e as coberturas vegetais sobre o teor de N foliar, número de panículas por m², matéria seca de plantas de arroz e a massa de cem grãos.Rice is an important source of energy and protein for the world population, mainly in Asia and Oceania. In Brazil, together with common beans, it is the population's basic food. Sustainable technologies that reduce rice production costs and increase productivity can warrant its supply on period of high demand. The objective of this study was to evaluate different preceding cover crops (millet [Pennisetum americanum

  5. Coberturas vegetais, doses de nitrogênio e inoculação de sementes com Azospirillum brasilense em arroz de terras altas no sistema plantio direto Cover crops, nitrogen rates and seeds inoculation with Azospirillum brasilense in upland rice under no-tillage

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    Douglas de Castilho Gitti

    2012-01-01

    Full Text Available O arroz é importante fonte de energia e proteínas para a população mundial, principalmente na Ásia e Oceania. No Brasil, juntamente com o feijão, constitui a base da alimentação. Tecnologias sustentáveis que reduzam custos da produção e aumentem a produtividade do arroz podem garantir seu suprimento em períodos de alta demanda. O objetivo deste trabalho foi avaliar diferentes coberturas vegetais antecessoras (milheto [Pennisetum americanum], crotalária [Crotalaria juncea], guandu [Cajanus cajan], braquiária [Brachiaria ruziziensis], milheto + crotalária e milheto + guandu, doses de nitrogênio (N em cobertura (0, 40, 80 e 120 kg ha-1 e o efeito da inoculação de sementes com Azospirillum brasilense no arroz de terras altas em sistema plantio direto no desenvolvimento e na produtividade. Utilizou-se o delineamento experimental em blocos casualizados em esquema fatorial 6x4x2 com quatro repetições. O estudo foi desenvolvido em um Latossolo Vermelho, em Selvíria, Estado do Mato Grosso do Sul, Brasil, em 2011/2012. O cultivo do guandu antecedendo o arroz proporcionou maior produtividade do arroz somente em comparação a B. ruziziensi. A produtividade do arroz em função das doses de N em cobertura se ajustou a uma função quadrática. Não houve influência da inoculação de sementes com A. brasilense sobre a produtividade do arroz, porém houve interação entre a inoculação e as coberturas vegetais sobre o teor de N foliar, número de panículas por m², matéria seca de plantas de arroz e a massa de cem grãos.Rice is an important source of energy and protein for the world population, mainly in Asia and Oceania. In Brazil, together with common beans, it is the population's basic food. Sustainable technologies that reduce rice production costs and increase productivity can warrant its supply on period of high demand. The objective of this study was to evaluate different preceding cover crops (millet [Pennisetum americanum

  6. Qualidade sensorial do café de lavouras em conversão para o sistema de produção orgânico Cup quality of traditional crop coffee converted to organic system

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    Marcelo Ribeiro Malta

    2008-01-01

    Full Text Available Com o objetivo de se verificar a qualidade sensorial do café de lavouras em conversão para o sistema de produção orgânico, foi instalado este experimento, no município de Lavras (MG. O experimento foi instalado em 2004, em uma lavoura de café Catuaí Amarelo IAC 86, espaçamento de 4,0 x 0,6 m, com seis anos de idade, anteriormente cultivada no sistema convencional. Nos tratamentos orgânicos, empregou-se o delineamento látice balanceado 4 x 4, com cinco repetições em esquema fatorial 3 x 2 x 2, mais quatro tratamentos adicionais. O fatorial constou da utilização de três fontes de matéria orgânica (farelo de mamona, cama de frango e esterco bovino, com ou sem aplicação de casca de café e de adubação verde com feijão-guandu (Cajanus cajan L. Millsp.. Os quatro tratamentos adicionais consistiram de: 1- esterco bovino + casca de café + moinha de carvão + sulfato duplo de potássio e magnésio; 2 - farelo de mamona + casca de café + farinha de rocha; 3 - casca de café e 4 - adubação verde. Para efeito de comparação, manteve-se no mesmo talhão uma lavoura com manejo convencional. Não foram observadas diferenças de qualidade de bebida entre os cafés submetidos aos diferentes tratamentos orgânicos, nem entre esses e a testemunha no primeiro ano de conversão. No segundo ano, observou-se superioridade das bebidas de cafés de alguns tratamentos orgânicos em relação ao da lavoura desenvolvida no sistema convencional. Verificou-se efeito positivo da utilização do esterco bovino isoladamente ou associado com a casca de café e adubação verde na qualidade sensorial do café.The main purpose of this study was to evaluate sensorial attributes of coffee grown under a transition cultivation system, from conventional to organic, in Lavras (MG. The experiment was established in 2004, in a conventional field with 6 year-old Catuaí Amarelo IAC86 coffee plants, planted using 4,0 X 0,6 m spacing system. The experimental

  7. Phytosociological aspects and weed management using cover crops on organic conilon coffee plantations / Aspectos fitossociológicos e manejo de plantas espontâneas utilizando espécies de cobertura em cafeeiro Conilon orgânico

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    José Antonio Azevedo Espindola

    2010-09-01

    Full Text Available Weeds can bring benefits to agriculture, but when incorrectly managed they can compete with commercial crops for resources. The objective of this work was to evaluate the effect that cover crops, associated with Coffea canephora cv. Conilon, imposes to the weed community. Cover crops were planted between the rows of a 6.5 years old organic coffee plantation spaced 2.0 x 1.5 m. The experiment was arranged in a complete randomized block design, with four replications, in a factorial scheme with the following treatments: control, Pennisetum glaucum and the legume plants: Canavalia ensiformis, Mucuna deeringiana and Cajanus cajan, with and without Rizobium inoculation. Cover crops dry weight and nutrient contents on coffee trees were determined. Weeds density, frequency, relative abundance, importance value index and plant similarity index were also determined. Twenty seven weed species were identified, with special emphasis on Bidens subalternans and Commelina benghalensis. Cover crops promote modifications on the succession dynamics of weeds and do not interfere with the development of the coffee trees. Canavalia ensiformis, Mucuna deeringiana and Pennisetum glaucum help on weed control.As plantas espontâneas competem com as culturas por recursos escassos, quando mal manejadas. No entanto, podem trazer benefícios à agricultura. O objetivo desse trabalho foi avaliar o efeito que plantas de cobertura, consorciadas com Coffea canephora cv. Conilon, impõem à comunidade de plantas espontâneas. Plantas de cobertura foram semeadas nas entrelinhas de um cafezal de 6,5 anos conduzido sob manejo orgânico, com espaçamento de 2,0 x 1,5 m. O delineamento experimental foi de blocos ao acaso, com quatro repetições, num arranjo fatorial com tratamentos adicionais: testemunha, milheto – Pennisetum glaucum, e as leguminosas feijão-de-porco – Canavalia ensiformis, mucuna-anã – Mucuna deeringiana, e feijão-guandu – Cajanus cajan, com e sem

  8. Biologic dinitrogen fixation and nutrient cycling in cover crops and their effect on organic Conilon coffeeFixação biológica de nitrogênio e ciclagem de nutrientes por plantas de cobertura e seus efeitos sobre café Conilon orgânico

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    José Antonio Azevedo Espindola

    2011-08-01

    Full Text Available Notwithstanding its relevance, studies regarding nutrient cycling and biological dinitrogen fixation in Conilon coffee (Coffee canephora cv. Conilon associated with cover plants are very scarce. Aiming to evaluate the contribution of cover crops for organic conilon production, a field experiment was carried out consisting of Pennisetum glaucum, and legume species Canavalia ensiformis, Mucuna deeringiana and Cajanus cajan (inoculated and non inoculated cultivated between coffee trees, and spontaneous vegetation as cover crops. The experiment was carried out in Espírito Santo State- Brazil, in a 6.5 years old coffee crop production system. Chemical analyses of soil and vegetative parts of spontaneous and cover crops, as well as coffee leaf nutrients concentration were performed. Biological Nitrogen Fixation (BNF was determined by the natural abundance method. BNF contributed with about 80% of the nitrogen accumulated by the leguminous plants, corresponding to 27 - 35 kg of N ha-1. Concentration and accumulation of nutrients varied among cover crops. Rhizobium inoculation did not influence nutrient cycling or BNF. Legume plants partially supplied the nitrogen requirements of Conilon coffee. No significant effect of the treatments was observed on the nutrient concentration of Conilon coffee or on plant growth.Apesar de sua relevância, estudos sobre a ciclagem de nutrientes e fixação biológica do nitrogênio (FBN em café Conilon (Coffea canephora cv. Conilon, associadas com plantas de cobertura, são escassos. Objetivou-se, com este trabalho avaliar a ciclagem de nutrientes, a FBN e o efeito que plantas de cobertura podem causar em lavoura de C. canephora cv. Conilon, sob manejo orgânico. O experimento foi conduzido no Estado do Espírito Santo - Brasil, em uma lavoura de café sob manejo orgânico, com 6,5 anos. Os tratamentos consistiram de testemunha (ausência de plantas de cobertura, Pennisetum glaucum e as leguminosas Canavalia

  9. Genotypic variability in physiological, biomass and yield response to drought stress in pigeonpea.

    Science.gov (United States)

    Vanaja, M; Maheswari, M; Sathish, P; Vagheera, P; Jyothi Lakshmi, N; Vijay Kumar, G; Yadav, S K; Razzaq, Abdul; Singh, Jainender; Sarkar, B

    2015-10-01

    Three pigeonpea (Cajanus cajan L. Millsp.) genotypes- GT-1, AKP-1 and PRG-158 with varying crop duration, growth habit and flowering pattern were evaluated for variability in their response for drought stress. Drought stress was imposed at initiation of flowering and the observations on biomass and seed yield parameters were recorded at harvest. The magnitude of response of individual component to drought stress was found to be genotype specific. Drought stress significantly decreased photosynthetic rate (PN), transpiration rate (Tr) and relative water content (RWC) in all the genotypes, however the magnitude of reduction differed with genotype. With drought stress, the reduction of PN was highest in GT-1 while reduction in Tr was highest in PRG-158. The genotype AKP-1, accumulated significantly higher concentrations of osmotic solutes especially proline under water deficit stress, this facilitated it to maintain higher relative water content (RWC) and lower malondialdehyde (MDA) content as compared to other genotypes. Drought stress also impacted biomass production and their partitioning to vegetative and reproductive components at harvest. There was significant variability between the genotypes for seed yield under drought stress while it was non-significant under well-watered condition. Drought stress enhanced flower drop and decreased flower to pod conversion resulting in reduced pod number and seed number in PRG-158 and GT-1. The genotype AKP-1 recorded superior performance for seed yield under stress environment due to its ability in maintaining pod and seed number as well as improved test weight (100 seed weight). Under drought stress, significant positive association of seed yield with proline, seed number, pod number and test weight clearly indicating their role in drought tolerance. PMID:26600680

  10. 应用灰色关联度法评价灌草复合型种植模式——以金沙江干热河谷区的云南永胜县为例%Application of Gray Relevant Analysis in Cultivation Mode Evaluation of Shrub-Grass Composite:A Case Study of Hot-Arid Valley of the Jinsha River in Yongsheng County, Yunnan

    Institute of Scientific and Technical Information of China (English)

    许岳飞; 毕玉芬; 金晶炜

    2008-01-01

    2005年在金沙江干热河谷区的云南永胜县退化山地进行灌草组合模式的筛选,以土壤改良效果评价8种灌草种植模式,确定最优灌草组合模式.应用加权灰色关联分析和聚类评价方法,以土壤有机质、全氮、全磷、全钾、碱解氮、速效磷、速效钾等为指标,对土壤生态恢复贡献值进行定量分析和聚类评价.结果表明:"木豆(Cajanus cajan(L.)Millsp.)+非洲狗尾草(Setaria sphacelata(Schumaeh.)Stapf & C.E.Hubb.cv.Narok)+高羊茅(Festuca arundinacea Schreb.cv.Fine Lawn)+大翼豆(Macroptilum atropurpureum(DE.)Urban)+自三叶(Trifolium repens L.cv.Haifa)+杂三叶(Trifolium hybridum L.)"灌草复合型种植模式关联度值r=0.773为最大,聚类分析为优,对土壤贡献值优于其他种植模式,适合于大面积推广,是金沙江干热河谷区退化山地土壤生态恢复的优化灌草复合型种植模式.

  11. COMPARAÇÃO DE ASPECTOS QUÍMICOS E TECNOLÓGICOS DE GRÃOS VERDES E MADUROS DE GUANDU COM OS DE FEIJÃO-COMUM E ERVILHA

    Directory of Open Access Journals (Sweden)

    Jairo Osvaldo CAZETTA

    2009-07-01

    Full Text Available

    RESUMO: O objetivo deste trabalho foi comparar os aspectos químicos e tecnológicos de grãos verdes e maduros de três variedades de guandu (Cajanus cajan com os de feijão (Phaseolus vulgaris e ervilha (Pisum sativum. Foram estudadas as características de hidratação e coação, os teores de cinzas, extrato etéreo, proteína bruta, amido, carboidratos solúveis totais e redutores, nitrogênio solúvel total e atividade antitríptica. As determinações químicas foram realizadas nas amostras cruas, cozidas e no caldo. Os grãos maduros do guandu Kaki apresentaram composição química e características tecnológicas compatíveis com as do feijão, a não ser uma pequena porção de hardshell. Os grãos verdes das três variedades de guandu apresentaram-se mais ricos em minerais e extrato etéreo, mas uma atividade antitríptica muito superior à da ervilha. Quando recém-colhidos, revelaram cocção semelhante à da ervilha, mas a secagem artificial prejudicou drasticamente esta característica. Não se observou relação clara entre a composição química e as características tecnológicas. PALAVRAS – CHAVE: Guandu; feijão; ervilha; hidratação; cocção; composição química; atividade antitríptica.

  12. Produção e comportamento reológico de exopolissacarídeos sintetizados por rizóbios isolados de guandu

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    Paulo Ivan Fernandes Júnior

    2010-12-01

    Full Text Available O objetivo deste trabalho foi avaliar a produção e o comportamento reológico de exopolissacarídeos (EPS produzidos por rizóbios isolados de nódulos de guandu [Cajanus cajan (L. Millsp.], e a similaridade desses isolados, pela técnica de análise de restrição do DNA ribossomal amplificado. As bactérias foram cultivadas em meio YM líquido e os EPS foram obtidos por precipitação com etanol gelado a partir do caldo de cultivo. Em seguida, eles foram recuperados por centrifugação, secados a vácuo, pesados e ressuspensos em água para avaliações reológicas. Os três isolados avaliados apresentaram diferenças na produção e na eficiência relativa da produção de EPS, com destaque para o isolado 8.1c. Os EPS demonstraram comportamento não newtoniano e pseudoplástico, porém também apresentaram diferenças na viscosidade aparente em uma mesma taxa de cisalhamento. Na taxa de cisalhamento de 1 s-1, os três isolados foram diferentes, enquanto na taxa de cisalhamento de 40 s-1, os isolados 53.5 e 30.6a2 foram iguais e diferiram do isolado 8.1c. A similaridade dos isolados foi condizente com os resultados de reologia dos EPS, em que bactérias que sintetizaram EPS com menor viscosidade aparente apresentaram maior similaridade

  13. Cajaninstilbene acid protects corticosterone-induced injury in PC12 cells by inhibiting oxidative and endoplasmic reticulum stress-mediated apoptosis.

    Science.gov (United States)

    Liu, Yamin; Shen, Shengnan; Li, Zongyang; Jiang, Yumao; Si, Jianyong; Chang, Qi; Liu, Xinmin; Pan, Ruile

    2014-12-01

    It has been reported that high corticosterone level could damage the normal hippocampal neurons both in vitro and in vivo. Furthermore, high concentration of corticosterone induced impair in PC12 cells has been widely used as in vitro model to screen neuroprotective agents. Cajaninstilbene acid (CSA), a natural stilbene isolated from Cajanus cajan leaves, has various activities. In present study, we investigated the effect of CSA on corticosterone-induced cell apoptosis and explored its possible signaling pathways in PC12 cells. We demonstrated that pretreatment with CSA at the concentrations of 1-8 μmol/L remarkably reduced the cytotoxicity induced by 200 μmol/L of corticosterone in PC12 cells by MTT, and further confirmed the neuroprotection by Hoechst 33342 and PI double staining and lactate dehydrogenase release (LDH) assay at the concentration of 8 μmol/L. Moreover, the cytoprotection of CSA was proved to be associated with the homeostasis of intracellular Ca(2+), relieving corticosterone-induced oxidative stress by decreasing the contents of ROS and malondialdehyde (MDA), increasing the activities of superoxide dismutase (SOD) and catalase (CAT), and the stabilization of ER stress via down-regulating the expression of ER chaperone protein glucose-regulated protein 78 (GRP78), ER stress associated transcription factor C/EBP homologous protein (CHOP/GADD153), and the X box-binding protein-1 (XBP-1), as well as the expression of ER stress-specific protein caspase-12 and its downstream protein caspase-9. Considering all the findings, it is suggested that the neuroprotective activity of CSA against the impairment induced by corticosterone in PC12 cells was through the inhibition of oxidative stress and ER stress-mediated pathway. PMID:25193317

  14. Efeito da suplementação com feno de Leucena (Leucaena leucocephala (Lam de Wit durante a estação seca sobre o desenvolvimento ponderal de ovinos Effect of supplementation with Leucaena leucocephala hay during the dry season on the ponderal development sheep

    Directory of Open Access Journals (Sweden)

    Antônio Alves de Souza

    1999-01-01

    Full Text Available O objetivo deste experimento foi estudar o desempenho de borregos suplementados com dois níveis de feno de leucena (Leucaena leucocephala (Lam de Wit, durante a estação seca, em comparação com animais mantidos em pastagem exclusiva de capim-buffel (Cenchrus ciliaris, L ou em pastagem consorciada de capim-buffel com guandu (Cajanus cajan. Durante a estação das águas, foi determinado ainda o desempenho dos animais que foram mantidos em pastagem comum de capim-buffel, sem suplementação. Os seguintes tratamentos foram testados durante a estação seca: A - pasto de capim-buffel; B - pasto de capim-buffel consorciado com guandu; C - pasto de capim-buffel suplementado com feno de leucena (250 g/animal•d; e D - pasto de capim-buffel suplementado com feno de leucena (500 g/animal•d. Durante a estação seca, somente os borregos suplementados com o nível mais alto de feno de leucena (tratamento D apresentaram ganho diário de peso mais elevado que os animais mantidos em dieta exclusiva de pasto (tratamentos A e B. Na estação das águas, os animais que apresentaram melhor desempenho durante a estação seca passaram a apresentar ganho diário de peso inferior em relação aos dos demais tratamentos. Quando as duas estações foram consideradas em conjunto, a vantagem com a suplementação desapareceu e não houve diferenças entre tratamentos. O uso de feno de leucena para suplementação de borregos mantidos a pasto, durante a estação seca, é eficiente, desde que os animais estejam terminados e sejam abatidos ao final da estação.

  15. Comparative sequence analysis of nitrogen fixation-related genes in six legumes.

    Science.gov (United States)

    Kim, Dong Hyun; Parupalli, Swathi; Azam, Sarwar; Lee, Suk-Ha; Varshney, Rajeev K

    2013-01-01

    Legumes play an important role as food and forage crops in international agriculture especially in developing countries. Legumes have a unique biological process called nitrogen fixation (NF) by which they convert atmospheric nitrogen to ammonia. Although legume genomes have undergone polyploidization, duplication and divergence, NF-related genes, because of their essential functional role for legumes, might have remained conserved. To understand the relationship of divergence and evolutionary processes in legumes, this study analyzes orthologs and paralogs for selected 20 NF-related genes by using comparative genomic approaches in six legumes i.e., Medicago truncatula (Mt), Cicer arietinum, Lotus japonicus, Cajanus cajan (Cc), Phaseolus vulgaris (Pv), and Glycine max (Gm). Subsequently, sequence distances, numbers of synonymous substitutions per synonymous site (Ks) and non-synonymous substitutions per non-synonymous site (Ka) between orthologs and paralogs were calculated and compared across legumes. These analyses suggest the closest relationship between Gm and Cc and the highest distance between Mt and Pv in six legumes. Ks proportional plots clearly showed ancient genome duplication in all legumes, whole genome duplication event in Gm and also speciation pattern in different legumes. This study also reports some interesting observations e.g., no peak at Ks 0.4 in Gm-Gm, location of two independent genes next to each other in Mt and low Ks values for outparalogs for three genes as compared to other 12 genes. In summary, this study underlines the importance of NF-related genes and provides important insights in genome organization and evolutionary aspects of six legume species analyzed. PMID:23986765

  16. Absorção de N, P e K por espécies de adubos verdes cultivadas em diferentes épocas e densidades num latossolo vermelho-escuro argiloso sob cerrados

    Directory of Open Access Journals (Sweden)

    R. F. Amabile

    1999-12-01

    Full Text Available Populações específicas de plantas e a época de semeadura podem promover interferências na absorção dos nutrientes. Assim, um experimento foi realizado, no ano agrícola de 1991/92, num Latossolo Vermelho-Escuro argiloso no Centro Nacional de Pesquisa de Solos/Embrapa, em Senador Canedo (GO, com o objetivo de determinar os teores e as quantidades absorvidas de nitrogênio, fósforo e potássio na parte aérea da Crotalaria juncea: l., Crotalaria ochroleuca: G. Don, mucuna preta ( Mucuna aterrima (Piper & Tracy Merr. e guandu cv. Kaki (Cajanus cajan (L. Millsp., em três épocas de semeadura (início, meados e final da estação chuvosa e em dois espaçamentos entrelinhas (0,5 e 0,4 m. O delineamento experimental, dentro de cada época, foi o de blocos ao acaso em parcelas subdivididas, com três repetições. As espécies diferiram entre si quanto aos teores e quantidades absorvidas de N, P e K. Ao contrário das épocas de semeadura, não houve efeito significativo dos espaçamentos nos teores e quantidades absorvidas de N, P e K. A interação de espécies e épocas de semeadura foi significativa apenas para o nitrogênio e fósforo.

  17. Comparative sequence analysis of nitrogen fixation-related genes in six legumes

    Directory of Open Access Journals (Sweden)

    Dong Hyun eKim

    2013-08-01

    Full Text Available Legumes play an important role as food and forage crops in international agriculture especially in developing countries. Legumes have a unique biological process called nitrogen fixation (NF by which they convert atmospheric nitrogen to ammonia. Although legume genomes have undergone polyploidization, duplication and divergence, NF-related genes, because of their essential functional role for legumes, might have remained conserved. To understand the relationship of divergence and evolutionary processes in legumes, this study analyzes orthologs and paralogs for selected 20 NF-related genes by using comparative genomic approaches in six legumes i.e. Medicago truncatula (Mt, Cicer arietinum, Lotus japonicus, Cajanus cajan (Cc, Phaseolus vulgaris (Pv and Glycine max (Gm. Subsequently, sequence distances, numbers of synonymous substitutions per synonymous site (Ks and nonsynonymous substitutions per nonsynonymous site (Ka between orthologs and paralogs were calculated and compared across legumes. These analyses suggest the closest relationship between Gm and Cc and the farthest distance between Mt and Pv in 6 legumes. Ks proportional plots clearly showed ancient genome duplication in all legumes, whole genome duplication event in Gm and also speciation pattern in different legumes. This study also reported some interesting observations e.g. no peak at Ks 0.4 in Gm-Gm, location of two independent genes next to each other in Mt and low Ks values for outparalogs for three genes as compared to other 12 genes. In summary, this study underlines the importance of NF-related genes and provides important insights in genome organization and evolutionary aspects of six legume species analyzed.

  18. Aspectos agronômicos de leguminosas para adubação verde no Cerrado do Alto Vale do Jequitinhonha

    Directory of Open Access Journals (Sweden)

    Ricardo Borges Teodoro

    2011-04-01

    Full Text Available O uso intensivo e inadequado dos solos acelera sua degradação, sendo necessária a intervenção por meio de práticas conservacionistas para restaurar a capacidade produtiva dos mesmos. O objetivo deste estudo foi avaliar o comportamento e desenvolvimento de diferentes leguminosas utilizadas como adubos verdes em solos de Cerrado, Alto Vale do Jequitinhonha, em Turmalina, MG. O delineamento experimental adotado foi em blocos ao acaso, com sete tratamentos e quatro repetições, sendo os tratamentos constituídos pelas leguminosas: mucuna-cinza (Mucuna nivea, mucuna-preta (Mucuna aterrima, lablabe (Dolichos lablab, feijão-de-porco (Canavalia ensiformis, Crotalaria juncea, Crotalaria spectabilis e guandu-anão (Cajanus cajan. O ciclo precoce de C . juncea, C. spectabilis e feijão-de-porco favorece a inserção destes nos sistemas de cultivo. Aos 40 dias, o feijão-de-porco e mucuna-cinza já cobriam o solo, com 67 e 63 %; já o guandu-anão e C. juncea apresentaram os maiores desenvolvimentos, nesse período. Os teores de N, P e K tendem a diminuir nas avaliações realizadas nas diferentes fases vegetativas, o que contribui para melhor escolha da época de manejo das leguminosas. Crotalaria juncea, mucuna-cinza, feijão-de-porco e mucuna-preta foram as leguminosas que se destacaram na produção de matéria seca, o que torna essas espécies promissoras para adubação verde na região. As leguminosas, em sua maioria, apresentam potencial para reciclagem dos macronutrientes e aporte de N aos sistemas de produção.

  19. Environmental Influences on Pigeonpea-Fusarium udum Interactions and Stability of Genotypes to Fusarium Wilt.

    Science.gov (United States)

    Sharma, Mamta; Ghosh, Raju; Telangre, Rameshwar; Rathore, Abhishek; Saifulla, Muhammad; Mahalinga, Dayananda M; Saxena, Deep R; Jain, Yogendra K

    2016-01-01

    Fusarium wilt (Fusarium udum Butler) is an important biotic constraint to pigeonpea (Cajanus cajan L.) production worldwide. Breeding for fusarium wilt resistance continues to be an integral part of genetic improvement of pigeonpea. Therefore, the study was aimed at identifying and validating resistant genotypes to fusarium wilt and determining the magnitude of genotype × environment (G × E) interactions through multi-environment and multi-year screening. A total of 976 genotypes including germplasm and breeding lines were screened against wilt using wilt sick plot at Patancheru, India. Ninety two genotypes resistant to wilt were tested for a further two years using wilt sick plot at Patancheru. A Pigeonpea Wilt Nursery (PWN) comprising of 29 genotypes was then established. PWN was evaluated at nine locations representing different agro-climatic zones of India for wilt resistance during two crop seasons 2007/08 and 2008/09. Genotypes (G), environment (E), and G × E interactions were examined by biplot which partitioned the main effect into G, E, and G × E interactions with significant levels (p ≤ 0.001) being obtained for wilt incidence. The genotype contributed 36.51% of resistance variation followed by the environment (29.32%). A GGE biplot integrated with a boxplot and multiple comparison tests enabled us to identify seven stable genotypes (ICPL 20109, ICPL 20096, ICPL 20115, ICPL 20116, ICPL 20102, ICPL 20106, and ICPL 20094) based on their performance across diverse environments. These genotypes have broad based resistance and can be exploited in pigeonpea breeding programs. PMID:27014287

  20. Global Synthesis of Drought Effects on Food Legume Production.

    Science.gov (United States)

    Daryanto, Stefani; Wang, Lixin; Jacinthe, Pierre-André

    2015-01-01

    Food legume crops play important roles in conservation farming systems and contribute to food security in the developing world. However, in many regions of the world, their production has been adversely affected by drought. Although water scarcity is a severe abiotic constraint of legume crops productivity, it remains unclear how the effects of drought co-vary with legume species, soil texture, agroclimatic region, and drought timing. To address these uncertainties, we collected literature data between 1980 and 2014 that reported monoculture legume yield responses to drought under field conditions, and analyzed this data set using meta-analysis techniques. Our results showed that the amount of water reduction was positively related with yield reduction, but the extent of the impact varied with legume species and the phenological state during which drought occurred. Overall, lentil (Lens culinaris), groundnut (Arachis hypogaea), and pigeon pea (Cajanus cajan) were found to experience lower drought-induced yield reduction compared to legumes such as cowpea (Vigna unguiculata) and green gram (Vigna radiate). Yield reduction was generally greater when legumes experienced drought during their reproductive stage compared to during their vegetative stage. Legumes grown in soil with medium texture also exhibited greater yield reduction compared to those planted on soil of either coarse or fine texture. In contrast, regions and their associated climatic factors did not significantly affect legume yield reduction. In the face of changing climate, our study provides useful information for agricultural planning and research directions for development of drought-resistant legume species to improve adaptation and resilience of agricultural systems in the drought-prone regions of the world. PMID:26061704

  1. PIPEMicroDB: microsatellite database and primer generation tool for pigeonpea genome.

    Science.gov (United States)

    Sarika; Arora, Vasu; Iquebal, M A; Rai, Anil; Kumar, Dinesh

    2013-01-01

    Molecular markers play a significant role for crop improvement in desirable characteristics, such as high yield, resistance to disease and others that will benefit the crop in long term. Pigeonpea (Cajanus cajan L.) is the recently sequenced legume by global consortium led by ICRISAT (Hyderabad, India) and been analysed for gene prediction, synteny maps, markers, etc. We present PIgeonPEa Microsatellite DataBase (PIPEMicroDB) with an automated primer designing tool for pigeonpea genome, based on chromosome wise as well as location wise search of primers. Total of 123 387 Short Tandem Repeats (STRs) were extracted from pigeonpea genome, available in public domain using MIcroSAtellite tool (MISA). The database is an online relational database based on 'three-tier architecture' that catalogues information of microsatellites in MySQL and user-friendly interface is developed using PHP. Search for STRs may be customized by limiting their location on chromosome as well as number of markers in that range. This is a novel approach and is not been implemented in any of the existing marker database. This database has been further appended with Primer3 for primer designing of selected markers with left and right flankings of size up to 500 bp. This will enable researchers to select markers of choice at desired interval over the chromosome. Furthermore, one can use individual STRs of a targeted region over chromosome to narrow down location of gene of interest or linked Quantitative Trait Loci (QTLs). Although it is an in silico approach, markers' search based on characteristics and location of STRs is expected to be beneficial for researchers. Database URL: http://cabindb.iasri.res.in/pigeonpea/ PMID:23396298

  2. Activity of the antiestrogenic cajanin stilbene acid towards breast cancer.

    Science.gov (United States)

    Fu, Yujie; Kadioglu, Onat; Wiench, Benjamin; Wei, Zuofu; Wang, Wei; Luo, Meng; Yang, Xiaohe; Gu, Chengbo; Zu, Yuangang; Efferth, Thomas

    2015-11-01

    Antiestrogenic therapy is a mainstay for estrogen receptor (ERα)-positive breast cancer. Due to the development of resistance to established antihormones such as tamoxifen, novel compounds are required. The low abundant cajanin stilbene acid (CSA) recently isolated by us from Pigeon Pea (Cajanus cajan) has structural similarities with estrogen. We analyzed the cytotoxic and anticancer activity of CSA in ERα-positive and -negative human breast cancer cells in vitro, in vivo and in silico. CSA exerts anticancer and antiestrogenic activities towards ERα-positive breast cancer, and it showed cytotoxicity towards tamoxifen-resistant MCF-7 cells, implying that CSA may be active against tamoxifen-resistant breast cancer cells. CSA showed low cytotoxicity in ERα-negative breast tumor cells as expected. Comparable cytotoxicity was observed towards p53 negative MCF-7 cells, implying that CSA is effective independent of the p53 status. Xenografted MCF-7 cells in nude mice were better inhibited by CSA than by cyclophosphamide. Testing of 8 primary cell cultures derived from human breast cancer biopsies showed that cell cultures from ER-positive tumors were more sensitive than from ER-negative ones. Dose-dependent decrease in ERα protein levels was observed upon CSA treatment. Synergistic effect with tamoxifen was observed in terms of increased p53 protein level. CSA affected pathways related to p53, cancer and cell proliferation. Gene promoter analyses supported the ERα regulation. CSA bound to the same site as 17β-estradiol and tamoxifen on ERα. In conclusion, CSA exerts its anticancer effects in ERα-positive breast cancer cells by binding and inhibiting ERα. PMID:26365581

  3. Desempenho agronômico de plantas de cobertura usadas na proteção do solo no período de pousio

    Directory of Open Access Journals (Sweden)

    Wellington Pereira de Carvalho

    2013-02-01

    Full Text Available O objetivo deste trabalho foi identificar espécies com alta produção de fitomassa, para proteção do solo no período de pousio. O experimento foi realizado com três espécies da família Leguminosae e três da família Poaceae, de 2009 a 2010, na área experimental do campus da Universidade Federal de Lavras, Minas Gerais. As espécies avaliadas foram: crotalária anagiroide (Crotalaria anagyroides, feijão‑de‑porco (Canavalia ensiformis, guandu‑anão (Cajanus cajan, aveia‑preta (Avena strigosa, sorgo (Sorghum bicolor e milheto (Pennisetum glaucum, semeadas em cultivo solteiro e consorciado. O feijão‑de‑porco e o sorgo apresentaram as maiores taxas de cobertura do solo em cultivo solteiro, enquanto o milheto apresentou a menor. O sorgo, em cultivo solteiro e consorciado com feijão‑de‑porco e guandu‑anão, apresenta a maior produção de matéria verde e matéria seca, enquanto o guandu‑anão apresenta a menor produção de matéria verde, e a crotalária anagiroide, a menor produção de matéria seca. Por ocasião da implantação da cultura comercial, o tratamento que proporcionou a maior quantidade de palha sobre o solo foi o consórcio entre feijão‑de‑porco e sorgo.

  4. Deep sequencing of dsRNAs recovered from mosaic-diseased pigeonpea reveals the presence of a novel emaravirus: pigeonpea sterility mosaic virus 2.

    Science.gov (United States)

    Elbeaino, Toufic; Digiaro, Michele; Uppala, Mangala; Sudini, Harikishan

    2015-08-01

    Deep-sequencing analysis of double-stranded RNA extracted from a mosaic-diseased pigeonpea plant (Cajanus cajan L., family Fabaceae) revealed the complete sequence of six emaravirus-like negative-sense RNA segments of 7009, 2229, 1335, 1491, 1833 and 1194 nucleotides in size. In the order from RNA1 to RNA6, these genomic RNAs contained ORFs coding for the RNA-dependent RNA polymerase (RdRp, p1 of 266 kDa), the glycoprotein precursor (GP, p2 of 74.5 kDa), the nucleocapsid (NC, p3 of 34.9 kDa), and the putative movement protein (MP, p4 of 40.7 kDa), while p5 (55 kDa) and p6 (27 kDa) had unknown functions. All RNA segments showed distant relationships to viruses of the genus Emaravirus, and in particular to pigeonpea sterility mosaic virus (PPSMV), with which they shared nucleotide sequence identity ranging from 48.5 % (RNA3) to 62.5 % (RNA1). In phylogenetic trees constructed from the sequences of the proteins encoded by RNA1, RNA2 and RNA3 (p1, p2 and p3), this new viral entity showed a consistent grouping with fig mosaic virus (FMV) and rose rosette virus (RRV), which formed a cluster of their own, clearly distinct from PPSMV-1. In experimental greenhouse trials, this novel virus was successfully transmitted to pigeonpea and French bean seedlings by the eriophyid mite Aceria cajani. Preliminary surveys conducted in the Hyderabad region (India) showed that the virus in question is widespread in pigeonpea plants affected by sterility mosaic disease (86.4 %) but is absent in symptomless plants. Based on molecular, biological and epidemiological features, this novel virus is the second emaravirus infecting pigeonpea, for which the provisional name pigeonpea sterility mosaic virus 2 (PPSMV-2) is proposed. PMID:26060057

  5. Ensifer glycinis sp. nov., a rhizobial species associated with species of the genus Glycine.

    Science.gov (United States)

    Yan, Hui; Yan, Jun; Sui, Xin Hua; Wang, En Tao; Chen, Wen Xin; Zhang, Xiao Xia; Chen, Wen Feng

    2016-09-01

    Rhizobial strains from root nodules of Astragalus mongholicus and soybean (Glycine max) were characterized phylogenetically as members of the genus Ensifer (formerly named Sinorhizobium), based on 16S rRNA gene sequence comparisons. Results based upon concatenated sequence analysis of three housekeeping genes (recA, atpD and glnII, ≤ 93.8 % similarities to known species) and average nucleotide identity (ANI) values of whole genome sequence comparisons (ranging from 89.6 % to 83.4 % to Ensifer fredii and Ensifer saheli, respectively) indicated the distinct positions of these novel strains within the genus Ensifer. Phylogeny of symbiotic genes (nodC and nifH) of three novel strains clustered them with rhizobial species Ensifer fredii and Ensifer sojae, both isolated from nodules of Glycine max. Cross-nodulation tests showed that the representative strain CCBAU 23380T could form root nodules with nitrogen fixation capability on Glycine soja, Albizia julibrissin, Vigna unguiculata and Cajanus cajan, but failed to nodulate Astragalus mongholicus, its original host legume. Strain CCBAU 23380T formed inefficient nodules on G. max, and it did not contain 18 : 0, 18 : 1ω7c 11-methyl or summed feature 1 fatty acids, which differed from other related strains. Failure to utilize malonic acid as a carbon source distinguished strain CCBAU 23380T from the type strains of related species. The genome size of CCBAU 23380T was 6.0 Mbp, comprising 5624 predicted genes with DNA G+C content of 62.4 mol%. Based on the results above, a novel species, Ensifer glycinis sp. nov., is proposed, with CCBAU 23380T (=LMG 29231T =HAMBI 3645T) as the type strain. PMID:27125987

  6. ADUBOS VERDES NA FITORREMEDIAÇÃO DE SOLOS CONTAMINADOS COM O HERBICIDA TEBUTHIURON

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    Fábio Ribeiro Pires

    2006-01-01

    Full Text Available The tebuthiuron residue, used in the sugar-cane culture, can be found in soil until two or more years after its application. Recent researches are being done using the phytoremediation in the attempt of removing it from the soil. The purpose of this work was to evaluate the effectiveness of the green manure use in the phyitoremediation of the tebuthiuron herbicide. The evaluated species were: Cajanus cajan, Canavalia ensiformis, Dolichos lablab, Pennisetum glaucum, Estizolobium deeringianum, Estizolobium aterrimum and Lupinus albus. These were sown and cultivated in pots containing soil treated with different doses of tebuthiuron (0.0; 0.5; 1.0; and 1.5 kg.ha-1. A control treatment without green manure, submitted to the same dosages, was kept. Sixty days after planting, green manure aerial part of all plants were harvested and soybean was sowed in the same pot, to bioassay accomplishment. Sixty days after sowing, the soybean plants were harvested, being the following evaluations carried out: height of plants, phytotoxicity symptoms and plants above ground dry biomass and leaf area. Until 0.5 kg.ha-1 tebuthiuron dosage, the species that better phytoremediated this herbicide was L. albus. When the soil was treated with tebuthiuron at 1.0 kg.ha-1, the C. ensiformis, followed by L. albus and S. aterrimum, they were the treatments that better phytoremediated tebuthiuron because results in highest plant height, above ground dry biomass and lesser phytotoxicity symptoms and still the biggest foliar area of the soybean plants. When the tebuthiuron was applied at 1.5 kg ha-1 dosage, it was impossible to evaluate the phyitoremediation, as the tested plants were eliminated.

  7. Ensifer glycinis sp. nov., a rhizobial species associated with species of the genus Glycine.

    Science.gov (United States)

    Yan, Hui; Yan, Jun; Sui, Xin Hua; Wang, En Tao; Chen, Wen Xin; Zhang, Xiao Xia; Chen, Wen Feng

    2016-09-01

    Rhizobial strains from root nodules of Astragalus mongholicus and soybean (Glycine max) were characterized phylogenetically as members of the genus Ensifer (formerly named Sinorhizobium), based on 16S rRNA gene sequence comparisons. Results based upon concatenated sequence analysis of three housekeeping genes (recA, atpD and glnII, ≤ 93.8 % similarities to known species) and average nucleotide identity (ANI) values of whole genome sequence comparisons (ranging from 89.6 % to 83.4 % to Ensifer fredii and Ensifer saheli, respectively) indicated the distinct positions of these novel strains within the genus Ensifer. Phylogeny of symbiotic genes (nodC and nifH) of three novel strains clustered them with rhizobial species Ensifer fredii and Ensifer sojae, both isolated from nodules of Glycine max. Cross-nodulation tests showed that the representative strain CCBAU 23380T could form root nodules with nitrogen fixation capability on Glycine soja, Albizia julibrissin, Vigna unguiculata and Cajanus cajan, but failed to nodulate Astragalus mongholicus, its original host legume. Strain CCBAU 23380T formed inefficient nodules on G. max, and it did not contain 18 : 0, 18 : 1ω7c 11-methyl or summed feature 1 fatty acids, which differed from other related strains. Failure to utilize malonic acid as a carbon source distinguished strain CCBAU 23380T from the type strains of related species. The genome size of CCBAU 23380T was 6.0 Mbp, comprising 5624 predicted genes with DNA G+C content of 62.4 mol%. Based on the results above, a novel species, Ensifer glycinis sp. nov., is proposed, with CCBAU 23380T (=LMG 29231T =HAMBI 3645T) as the type strain.

  8. Development of dominant sequence characterized amplified region (SCAR marker linked with plume moth (Exelastis atomosa Walsingham 1886 resistance in pigeon-pea

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    Ramya R Mishra

    2015-12-01

    Full Text Available The mode of gene action governing resistance to plume moth (Exelastis atomosa Walsingham 1886 derived from pigeon-pea (Cajanus scarabaeoides (L. Thouars accession ICPW-94 has been determined and the resistance alleles have been designated as PPM1. The progenies of F2 population and F3 families derived from an interspecific cross C. cajan (L. Huth ('ICP-26' x C. scarabaeoides (accession ICPW-94 revealed monogenic gene action for resistance to plume moth, and the dominant control by single locus or cluster of tightly linked alleles. Bulked segregant analysis (BSA of 116 F2 progenies by using 143 parental polymorphic RAPD primers could identify a fragment OPA09(910 associated with plume moth resistance in coupling phase of linkage. Further single plant analysis of the 116 F2 mapping population revealed OPA09(910 was linked to PPMi locus conferring host resistance to plume moth with recombination fraction (rf value of 0.125 (12.7 cM of Kosambi function. The resistance specific fragment OPA09(910 was cloned, sequenced and converted into a sequence characterized amplified region (SCAR marker, SCOPA09(942, which was also closely associated (10.3 cM with the locus PPMl with rf value 0.102. BLAST analysis with pigeon-pea genome sequence also confirmed its occurrence in CcLG02 (Scafseq.LG_V5.0fa and contig 01597 (AFSP01.fsa1. This SCAR marker showed reasonable screening efficiency in the F2, F3, and BC1F1 lines, thus it can be used as genetic handle in marker-assisted introgression of the genomic fragment conferring plume moth resistance and screening of breeding lines in pigeon-pea.

  9. Legumes and forage species sole or intercropped with corn in soybean-corn succession in midwestern Brazil

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    Gessí Ceccon

    2013-02-01

    Full Text Available The feasibility of no-tillage in the Cerrado (Savanna-like vegetation of Brazil depends on the production of sufficient above-ground crop residue, which can be increased by corn-forage intercropping. This study evaluated how above-ground crop residue production and yields of soybean and late-season corn in a soybean-corn rotation were influenced by the following crops in the year before soybean: corn (Zea mays L. intercropped with Brachiaria (Urochloa brizantha cv. Marandu, B. decumbens cv. Basilisk, B. ruziziensis, cv. comum., Panicummaximum cv. Tanzânia, sunn hemp (Crotalaria juncea L., pigeon pea [Cajanus cajan (L. Millsp]; sole corn, forage sorghum [Sorghum bicolor (L. Moench (cv. Santa Elisa], and ruzi grass. In March 2005, corn and forage species were planted in alternate rows spaced 0.90 m apart, and sole forage species were planted in rows spaced 0.45 m apart. In October 2005, the forages were killed with glyphosate and soybean was planted. After the soybean harvest in March 2006, sole late-season corn was planted in the entire experimental area. Corn grain and stover yields were unaffected by intercropping. Above-ground crop residue was greater when corn was intercropped with Tanzania grass (10.7 Mg ha-1, Marandu (10.1 Mg ha-1, and Ruzi Grass (9.8 Mg ha-1 than when corn was not intercropped (4.0 Mg ha-1. The intercropped treatments increased the percentage of soil surface covered with crop residue. Soybean and corn grain yields were higher after sole ruzi grass and intercropped ruzi grass than after other crops. The intercropping corn with Brachiaria spp. and corn with Panicum spp. increases above-ground crop residue production and maintains nutrients in the soil without reducing late-season corn yield and the viability of no-till in the midwestern region of Brazil.

  10. Cajaninstilbene acid relaxes rat renal arteries: roles of Ca2+ antagonism and protein kinase C-dependent mechanism.

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    Dong-Mei Zhang

    Full Text Available Cajaninstilbene acid (CSA is a major active component present in the leaves of Cajanus cajan (L. Millsp. The present study explores the underlying cellular mechanisms for CSA-induced relaxation in rat renal arteries. Vascular reactivity was examined in arterial rings that were suspended in a Multi Myograph System and the expression of signaling proteins was assessed by Western blotting method. CSA (0.1-10 µM produced relaxations in rings pre-contracted by phenylephrine, serotonin, 9, 11-dideoxy-9α, 11α-epoxymethanoprostaglandin F(2α (U46619, and 60 mM KCl. CSA-induced relaxations did not show difference between genders and were unaffected by endothelium denudation, nor by treatment with N(G-nitro-L-arginine methyl ester, indomethacin, ICI-182780, tetraethylammonium ion, BaCl(2, glibenclamide, 4-aminopyridine or propranolol. CSA reduced contraction induced by CaCl(2 (0.01-5 mM in Ca(2+-free 60 mM KCl solution and by 30 nM (--Bay K8644 in 15 mM KCl solution. CSA inhibited 60 mM KCl-induced Ca(2+ influx in smooth muscle of renal arteries. In addition, CSA inhibited contraction evoked by phorbol 12-myristate 13-acetate (PMA, protein kinase C agonist in Ca(2+-free Krebs solution. Moreover, CSA reduced the U46619- and PMA-induced phosphorylation of myosin light chain (MLC at Ser19 and myosin phosphatase target subunit 1 (MYPT1 at Thr853 which was associated with vasoconstriction. CSA also lowered the phosphorylation of protein kinase C (PKCδ at Thr505. In summary, the present results suggest that CSA relaxes renal arteries in vitro via multiple cellular mechanisms involving partial inhibition of calcium entry via nifedipine-sensitive calcium channels, protein kinase C and Rho kinase.

  11. Sample size for morphological traits of pigeonpea

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    Giovani Facco

    2015-12-01

    Full Text Available The objectives of this study were to determine the sample size (i.e., number of plants required to accurately estimate the average of morphological traits of pigeonpea (Cajanus cajan L. and to check for variability in sample size between evaluation periods and seasons. Two uniformity trials (i.e., experiments without treatment were conducted for two growing seasons. In the first season (2011/2012, the seeds were sown by broadcast seeding, and in the second season (2012/2013, the seeds were sown in rows spaced 0.50 m apart. The ground area in each experiment was 1,848 m2, and 360 plants were marked in the central area, in a 2 m × 2 m grid. Three morphological traits (e.g., number of nodes, plant height and stem diameter were evaluated 13 times during the first season and 22 times in the second season. Measurements for all three morphological traits were normally distributed and confirmed through the Kolmogorov-Smirnov test. Randomness was confirmed using the Run Test, and the descriptive statistics were calculated. For each trait, the sample size (n was calculated for the semiamplitudes of the confidence interval (i.e., estimation error equal to 2, 4, 6, ..., 20% of the estimated mean with a confidence coefficient (1-? of 95%. Subsequently, n was fixed at 360 plants, and the estimation error of the estimated percentage of the average for each trait was calculated. Variability of the sample size for the pigeonpea culture was observed between the morphological traits evaluated, among the evaluation periods and between seasons. Therefore, to assess with an accuracy of 6% of the estimated average, at least 136 plants must be evaluated throughout the pigeonpea crop cycle to determine the sample size for the traits (e.g., number of nodes, plant height and stem diameter in the different evaluation periods and between seasons. 

  12. Economic Viability of Small Scale Organic Production of Rice, Common Bean and Maize in Goias State, Brazil

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    Alcido Elenor Wander

    2007-04-01

    Full Text Available This study was conducted to assess the economic feasibility of small scale organic production of rice, common bean and maize in Goias State, Brazil. During 2004/05 and 2005/06 growing seasons, rice, common bean and maize were produced at the organic farm of Embrapa Rice and Beans in five mulching systems (fallow, Crotalaria juncea, Cajanus cajan, Mucuna aterrima and Sorghum bicolor , with and without tillage. Soil tillage consisted of heavy disc harrowing followed by light disc harrowing. All operations and used inputs were recorded. Based on those records, the production costs for each crop were estimated for each cropping season. The costs included operations like sowing, ploughing, harrowing, spraying, fertilizer broadcasting and harvesting, as well as inputs like seeds, inoculant strains of Rhizobium, neem oil and organic fertilizers. The benefits include the gross revenue obtained by multiplying the production amount with the market price for non-organic products. For the purpose of analysis of competitiveness of organic production in comparison to conventional farming the market prices assumed were those of conventional production. In the analysis, the costs of certification were not considered yet due to lack of certifiers in the region. For comparison between traits, net revenue, the benefit-cost-ratio (BCR and the break even point were used. In 2004/05 growing season the BCR varied from 0.27 for common bean on S. bicolor mulch system with tillage up to 4.05 for green harvested maize produced after C. juncea in no tillage system. Common bean and rice were not economically viable in this growing season. In 2005/06 growing season the BCR varied between 0.75 for common bean after S. bicolor in tillage system and 4.50 for green harvested maize produced after fallow in no tillage system. In this season common bean was economically viable in leguminous mulching systems and green harvested maize was viable in all mulching systems.

  13. Importance of canopy attributes in determining dry matter accumulation of pigeonpea under contrasting moisture regimes

    International Nuclear Information System (INIS)

    Variability in the yield of extra-short-duration (ESD) pigeonpea [Cajanus cajan (L.) Huth.] genotypes in drought environments is related to variation in crop growth rate (CGR) and total dry matter (TDM) production. Our investigation was aimed at assessing the importance of canopy attributes in determining the growth of ESD pigeonpea under contrasting moisture regimes. Using two automated rain-out shelters (ROS), six genotypes were grown under well watered conditions or with water deficit imposed from flowering until maturity. Water deficit significantly decreased the cumulative intercepted photosynthetically active radiation (CIR). The relationship between biomass accumulation and CIR was linear and water deficit affected the slope of the relationship (i.e., radiation use efficiency, RUE) (b = 1.92 g MJ-1 for well watered vs. 1.43 g MJ-1 for water deficit). Genotypes differed in RUE under well watered (1.70 to 2.19 g MJ-1) and moisture deficit (1.30 to 1.66 g MJ-1) conditions. Genotypic variation in canopy attributes was significant. Leaf area duration (LAD) significantly correlated with CGR only under well watered conditions. Cumulative intercepted radiation and RUE accounted for nearly 99% of the genotypic variation in CGR under both moisture regimes, of which RUE alone contributed nearly 90%. Variation among genotypes in CIR alone did not explain the differences in dry matter accumulation under either moisture regime. Only RUE explained more than 90% of the genotypic variation in CGR and 70% in TDM under both moisture regimes. The results indicated that RUE is critical in determining pigeonpea productivity under well watered and moisture-deficit regimes

  14. The potential use of legume-based diets supplemented with microbial phytase on the growth performance and feed efficiency of sea bass, Lates calcarifer

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    Erlinda S. Ganzon-Naret

    2013-09-01

    Full Text Available A feeding trial was conducted for 12 weeks to evaluate the potential use of legume-based diets supplemented with dietary microbial phytase on the growth performance and feed efficiency of juvenile sea bass, Lates calcarifer. Fifteen sea bass juveniles (mean initial weight of 0.96 g and mean initial total length (TL of 4.2 cm were stocked at three replicates into each of the twelve 100 L conical fibreglass tanks containing 90 L sea water in a closed recirculating system with filtered and aerated sea water. Four isonitrogenous, isolipidic and isocaloric experimental diets were formulated. The control diet (C0 contained fish meal, soybean meal, shrimp meal and squid meal as major protein sources. Legume seed meals of pigeon pea (Cajanus cajan, yellow mungbeans (Phaseolus aureus, and green mungbeans (Vigna radiata were incorporated in the practical diets D1, D2 and D3 respectively at 18-20% replacing an equivalent amount of 6-7g fish meal protein and supplemented with microbial phytase at the level of 300U kg-1 diet. Growth rate, feed conversion ratio (FCR, protein efficiency ratio (PER and apparent net protein utilization (ANPU of sea bass were significantly (P < 0.05 higher in control diet than those given different legume based diets supplemented with phytase. Histological examination of the liver tissues for the different dietary treatments did not manifest any abnormalities. Phytase supplementation also improved bone ash, phosphorus (P concentration as well as P content in the carcass for fish in legume fed groups. Results from the present study showed that incorporation of dietary microbial phytase in legume based diets slightly improve the growth performance and P availability in sea bass juveniles.

  15. The potential use of legume-based diets supplemented with microbial phytase on the growth performance and feed efficiency of sea bass, Lates calcarifer

    Directory of Open Access Journals (Sweden)

    Erlinda S. Ganzon-Naret

    2013-09-01

    Full Text Available A feeding trial was conducted for 12 weeks to evaluate the potential use of legume-based diets supplemented with dietary microbial phytase on the growth performance and feed efficiency of juvenile sea bass, Lates calcarifer. Fifteen sea bass juveniles (mean initial weight of 0.96 g and mean initial total length (TL of 4.2 cm were stocked at three replicates into each of the twelve 100 L conical fibreglass tanks containing 90 L sea water in a closed recirculating system with filtered and aerated sea water. Four isonitrogenous, isolipidic and isocaloric experimental diets were formulated. The control diet (C0 contained fish meal, soybean meal, shrimp meal and squid meal as major protein sources. Legume seed meals of pigeon pea (Cajanus cajan, yellow mungbeans ((Phaseolus aureus,and green mungbeans ((Vigna radiata were incorporated in the practical diets D1, D2 and D3 respectively at 18-20% replacing an equivalent amount of 6-7g fish meal protein and supplemented with microbial phytase at the level of 300U kg-1 diet. Growth rate, feed conversion ratio (FCR, protein efficiency ratio (PER and apparent net protein utilization (ANPU of sea bass were significantly (P < 0.05 higher in control diet than those given different legume based diets supplemented with phytase. Histological examination of the liver tissues for the different dietary treatments did not manifest any abnormalities. Phytase supplementation also improved bone ash, phosphorus (P concentration as well as P content in the carcass for fish in legume fed groups. Results from the present study showed that incorporation of dietary microbial phytase in legume based diets slightly improve the growth performance and P availability in sea bass juveniles.

  16. Medicinal plants used for dogs in Trinidad and Tobago.

    Science.gov (United States)

    Lans, C; Harper, T; Georges, K; Bridgewater, E

    2000-06-12

    This paper documents ethnoveterinary medicines used to treat dogs in Trinidad and Tobago. In 1995, a 4-stage process was used to conduct the research and document the ethnoveterinary practices. Twenty-eight ethnoveterinary respondents were identified using the school-essay method, which is a modified rapid rural appraisal (RRA) technique. Semi-structured interviews were held with these respondents as well as with 30 veterinarians, 27 extension officers and 19 animal-health assistants and/or agricultural officers, and the seven key respondents that they identified. The final step involved hosting four participatory workshops with 55 of the respondents interviewed to discuss the ethnoveterinary data generated from the interviews and to determine dosages for some of the plants mentioned. Supplementary interviews were conducted in 1997 and 1998. Seeds of Carica papaya, and leaves of Cassia alata, Azadirachta indica, Gossypium spp., Cajanus cajan and Chenopodium ambrosiodes are used as anthelmintics. The anthelmintics Gossypium spp. and Chenopodium ambrosiodes are the most frequently used species. Crescentia cujete pulp, Musa spp. stem exudate, the inside of the pods of Bixa orellana, leaves of Cordia curassavica and Eclipta alba plant tops are used for skin diseases. Musa spp. stem exudate, seeds of Manilkara zapota, Pouteria sapota and Mammea americana and leaves of Cordia curassavica, Scoparia dulcis and Nicotiana tabacum are used to control ectoparasites. Dogs are groomed with the leaves of Cordia curassavica, Bambusa vulgaris and Scoparia dulcis. Psidium guajava buds and leaves and the bark of Anacardium occidentale are used for diarrhoea. Owners attempt to achieve milk let-down with a decoction of the leaves of Stachytarpheta jamaicensis. The plant uses parallel those practised in human folk medicine in other Caribbean countries and in other tropical countries. PMID:10821961

  17. A survey of the nutritional and haemagglutination properties of legume seeds generally available in the UK.

    Science.gov (United States)

    Grant, G; More, L J; McKenzie, N H; Stewart, J C; Pusztai, A

    1983-09-01

    Eighty-five samples from fifteen different legume seed lines generally available in the UK were examined by measurements of their net protein utilization by rats and by haemagglutination tests with erythrocytes from a number of different animal species. From these results the seeds were classified into four broad groups. Group a seeds from most varieties of kidney (Phaseolus vulgaris), runner (Phaseolus coccineus) and tepary (Phaseolus acutifolius) beans showed high reactivity with all cell types and were also highly toxic. Group b, which contained seeds from lima or butter beans (Phaseolus lunatus) and winged bean (Psophocarpus tetragonolobus), agglutinated only human and pronase-treated rat erythrocytes. These seeds did not support proper growth of the rats although the animals survived the 10 d experimental period. Group c consisted of seeds from lentils (Lens culinaris), peas (Pisum sativum), chick-peas (Cicer arietinum), blackeyed peas (Vigna sinensis), pigeon peas (Cajanus cajan), mung beans (Phaseolus aureus), field or broad beans (Vicia faba) and aduki beans (Phaseolus angularis). These generally had low reactivity with all cells and were non-toxic. Group d, represented by soya (Glycine max) and pinto (Phaseolus vulgaris) beans, generally had low reactivity with all cells but caused growth depression at certain dietary concentrations. This growth depression was probably mainly due to antinutritional factors other than lectins. Lectins from group a seeds showed many structural and immunological similarities. However the subunit composition of the lectin from the tepary bean samples was different from that of the other bean lectins in this or any other groups. PMID:6615758

  18. Carbon dioxide and light responses of photosynthesis in cowpea and pigeonpea during water deficit and recovery

    Energy Technology Data Exchange (ETDEWEB)

    Lopez, F.B.; Setter, T.L.; McDavid, C.R.

    1987-10-01

    Greenhouse-grown pigeonpea (Cajunus cajan, (L.)) and cowpea (Vigna unguiculata, (L.)) were well-watered or subjected to low water potential by withholding water to compare their modes of adaptation to water-limited conditions. Leaf CO/sub 2/ exchange rate (CER), leaf diffusive conductance to CO/sub 2/ (g/sub L/), and CO/sub 2/ concentration in the leaf intercellular air space (C/sub i/) were determined at various CO/sub 2/ concentrations and photon flux densities (PFD) of photosynthetically active radiation. In cowpea, g/sub L/ declined to less than 15% of controls and total water potential (Psi/sub w/) at midafternoon declined to -0.8 megapascal after 5 days of withholding water, whereas g/sub L/ in pigeonpea was about 40% of controls even though midafternoon Psi/sub w/ was -1.9 megapascal. After 8 days of withholding water, Psi/sub w/ at midafternoon decline to -0.9 and -2.4 megapascals in cowpea and pigeonpea, respectively. The solute component of water potential (Psi/sub s/) decreased substantially less in cowpea than pigeonpea. Photosynthetic CER at saturation photon flux density (PFD) and ambient external CO/sub 2/ concentration on day 5 of withholding decreased by 83 and 55% in cowpea and pigeonpea, respectively. When measured at external, CO/sub 2/ concentration in bulk air of 360 microliters per liter, the CER of cowpea had fully recovered to control levels 3 days after rewatering; however, at 970 microliters per liter the PFD-saturated CERS of both species were substantially lower than in controls, indicating residual impairment.

  19. INDEKS GLISEMIK KACANG-KACANGAN [Glycemic Index of Selected Legumes

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    Y. Marsono 1

    2002-12-01

    Full Text Available Nutritional management for diabetic patients based on selection of low available carbohydrate foods has been criticized because the same availability of carbohydrate in different foods may result in different degree of glycemic response. This management is now being corrected by additional aid in selecting foods with the glycemic index (GI of foods. GI is a measure of the glycemic response to the carbohydrate component within a food relative to the response to an equal carbohydrate portion of reference food (glucose or white bread. In Indonesia, data of the glycemic index of foods is still very limited. The objectives of the research are to provide GI of selected legumes, including red bean (Vigna umbellata, Mung bean (Phaseolus aureus, cow pea (Vigna sinensis ENDL, pigeon pea (Cajanus cajan MILLSPAUGH, edible podded peas (Pisum sativum LINN and soy bean (Glycine max MERR. Eleventh health and normal volunteers (not diabetic were provided. The volunteers took an overnight fasting, blood were drawn in the morning and analyzed for serum glucose. Then they were given the test legumes containing total carbohydrates equivalent to 25-g glucose to be consumed. Blood samples were drawn for glucose measurement every 30 minutes until 120 min after meal. Serum glucose was determined enzymatically and the glucose responses were drawn graphically. The GI of the beans studied was lowest for red bean (26 and highest for mung bean (76, Edible podded pea and soy bean had similar value of GI i.e. 30 and 31; whereas pigeon and cow pea had a higher value i.e. 35 and 51, respectively.

  20. A BRIEF REVIEW ON DISSIPATION PATTERN OF INSECTICIDES ON PIGEONPEA

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    G Priyadarshini

    2013-12-01

    Full Text Available Pigeonpea (Cajanus cajan L. Millsp. is an important pulse crop in the semi-arid tropics and subtropical farming systems, providing high quality vegetable protein, animal feed and firewood (Mittal and Ujagir, 2005. Indiscriminate use of pesticides led to the hazardous impacts of polluting food stuffs, water, air, soil and even human body. As a result, the beneficial effects of pesticides often get diluted due to the possible health hazards caused by their toxic residues left over via bio-magnification of their residues through the food chain and decomposition of same in the body fat. Since pigeonpea is a food crop, it may carry residues which warrant judicious use of pesticides in respect of persistence, dissipation, metabolism, movement and accumulation of residues. The analysis of pesticide residues on or in the pod is therefore essential to avoid the health hazards to the consumers by prescribing the waiting periods so that residues get dissipated down to the prescribed Maximum Residue Limits (MRL before consumption to avoid toxic hazards. One of the important reasons for change from chemical pest control to IPM is to rationalize the use of need based insecticides so as to minimize the contamination of feed and various components of environment, as these in turn pose toxicity hazards to all forms of life including man. It is therefore, necessary to monitor the pesticide residues in food commodities and to evaluate the safety of commonly used pesticides in crops. Therefore, keeping in view of the above discussions the available literature related to the dissipation of insecticides in pigeonpea has been reviewed.

  1. SOBREVIVÊNCIA E CRESCIMENTO DE SETE ESPÉCIES ARBÓREAS NATIVAS EM UMA ÁREA DEGRADADA DE FLORESTA ESTACIONAL DECIDUAL, NORTE DE MINAS GERAIS1

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    Yule Roberta Ferreira Nunes

    2015-10-01

    Full Text Available RESUMO Este trabalho objetivou avaliar o crescimento e sobrevivência de sete espécies arbóreas nativas em diferentes tratamentos, em uma área degradada na Reserva da COPASA (Juramento, MG. Para tal, foram utilizados aproximadamente 1,2 ha da área, onde foram plantadas 899 mudas, de sete espécies nativas (Anadenanthera colubrina, Copaifera langsdorffii, Dilodendron bipinatum, Myracrodruon urundeuva, Pterogyne nitens, Schinopsis brasiliensis e Senegalia polyphylla, acompanhadas durante 24 meses. A área foi dividida em cinco parcelas de 0,24 ha, que representaram os seguintes tratamentos: (T1 parcela semeada com capim (Brachiaria sp.; (T2 parcela onde as covas foram tratadas com condicionador de solo; (T3 parcela semeada com capim consorciado com leguminosa (Cajanus cajan; (T4 parcela semeada com leguminosa; e (T5 controle. A porcentagem de mortalidade foi maior no T2 e menor no T3, sendo as espécies C. langsdorffii (43,66% e S. brasiliensis (11,64% aquelas com maior e menor porcentagem de mortalidade, respectivamente. O crescimento (altura e diâmetro das mudas foi maior no T2 (24,32 ± 26,05 cm e 0,51 ± 0,37 mm, respectivamente e menor no T1 (10,82 ± 22,57 cm e 0,26 ± 0,27 mm; respectivamente. As espécies com maior crescimento em altura foram A. colubrina e S. polyphylla, já D. bipinatum e S. brasiliensis apresentaram maior crescimento em diâmetro. Pterogyne nitens apresentou o menor crescimento, além de alta mortalidade, mostrando baixa capacidade de estabelecimento em ambientes degradados.

  2. Nutrientes minerais na biomassa da parte aérea em culturas de cobertura de solo

    Directory of Open Access Journals (Sweden)

    Borkert Clóvis Manuel

    2003-01-01

    Full Text Available O objetivo deste trabalho foi estimar as quantidades de nutrientes reciclados por cinco espécies vegetais utilizadas como culturas de cobertura do solo e que podem retornar ao solo pela mineralização da biomassa. Foram coletadas de vários experimentos amostras da matéria verde de aveia-preta (Avena strigosa Schreb, mucu-napreta (Stizolobium aterrimum Piper & Tracy, guandu (Cajanus cajan (L. Millsp, tremoço (Lupinus albus L. e L. angustifolius L. e ervilhaca (Vicia sativa L.. Foi estimado o rendimento de matéria seca e determinados os teores de N, P, K, Ca, Mg, Mn, Zn, Cu, e, a partir dessas concentrações, foram calculadas a média observada, a média estimada e o intervalo de confiança a 95% para cada nutriente dentro de cada classe de rendimento de matéria seca, em cada espécie vegetal. Os dados foram tabulados dentro de intervalos de classe de rendimento de matéria seca e apresentadas as quantidades estimadas de nutrientes minerais. Foram ajustadas equações para estimar as quantidades desses nutrientes. A aveia-preta e a ervilhaca reciclam grande quantidade de K, e a ervilhaca, a mucu-napreta, o tremoço e o guandu reciclam grande quantidade de N. Todas as espécies reciclam quantidades apreciáveis de Ca, Mg e micronutrientes, porém baixas quantidades de P. A rotação de culturas é um meio de implementar com sucesso o aumento das áreas de lavoura em semeadura direta.

  3. Morphology of Some Species in the Subfamily Papilionoideae

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    Joan Adeola OWOLABI

    2016-06-01

    Full Text Available Morphological study of ten species in the subfamily Papilionoideae was carried out with the view to documenting diagnostic characters that would distinguish or group the species. The species studied belong to four tribes, namely: tribe Desmodieae – Desmodium tortuosum (Sw. DC., Desmodium scorpiurus (Sw. Desv., Desmodium adscendens (Sw. DC., tribe Phaseoleae – Cajanus cajan (L. Millsp., Calopogonium mucunoides Desv., Centrosema molle (Mart. ex. Benth., Mucuna pruriens (Linn. Walp., Vigna unguiculata (Linn. Walp., tribe Crotalarieae – Crotalaria retusa Linn., tribe Robinieae – Gliricidia sepium (Jacq. Walp. Qualitative and quantitative traits which had not been documented in previous works, especially in Nigeria, were studied. These include plant life span; leaf/leaflet apex, base, margin and pubescence; stem type, colour, shape and pubescence; sepal colour and pubescence; nature of margin of petal standard and presence or absence of pedicel; fruit colour, pubescence, tip and shape; seed colour, shape, surface and presence or absence of prominent hilum on the seed; number of seeds per fruit; pedicel length; length and width of petal standard, keel and wing. Characters of taxonomic value documented in this study were leaf type, leaf shape, leaf base, petiole type, stem type, seed shape, petal standard length, petal keel length and petal wing width. Data were subjected to one - way analysis of variance using Duncan’s multiple range test. It was noted that the important characters that can be used in establishing taxonomic relationship in the sub-family Papilionoideae were leaf type, leaf shape, leaf base, petiole type, stem shape, petal colour, petal margin and seed shape.

  4. Chemical composition and ruminal degradation kinetics of crude protein and amino acids, and intestinal digestibility of amino acids from tropical forages

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    Lidia Ferreira Miranda

    2012-03-01

    Full Text Available The objective of this research was to determine the chemical composition and ruminal degradation of the crude protein (CP, total and individual amino acids of leaves from tropical forages: perennial soybean (Neonotonia wightii, cassava (Manihot esculenta, leucaena (Leucaena leucocephala and ramie (Boehmeria nivea, and to estimate the intestinal digestibility of the rumen undegradable protein (RUDP and individual amino acids of leaves from the tropical forages above cited, but including pigeon pea (Cajanus cajan. Three nonlactating Holstein cows were used to determine the in situ ruminal degradability of protein and amino acids from leaves (6, 18 and 48 hours of ruminal incubation. For determination of the intestinal digestibility of RUDP, the residue from ruminal incubation of the materials was used for 18 hours. A larger concentration of total amino acids for ramie and smaller for perennial soybean were observed; however, they were very similar in leucaena and cassava. Leucine was the essential amino acid of greater concentration, with the exception of cassava, which exhibited a leucine concentration 40.45% smaller. Ramie showed 14.35 and 22.31% more lysine and methionine, respectively. The intestinal digestibility of RUDP varied from 23.56; 47.87; 23.48; 25.69 and 10.86% for leucaena, perennial soybean, cassava, ramie and pigeon pea, respectively. The individual amino acids of tropical forage disappeared in different extensions in the rumen. For the correct evaluation of those forages, one should consider their composition of amino acids, degradations and intestinal digestibility, once the amino acid composition of the forage does not reflect the amino acid profiles that arrived in the small intestine. Differences between the degradation curves of CP and amino acids indicate that degradation of amino acids cannot be estimated through the degradation curve of CP, and that amino acids are not degraded in a similar degradation profile.

  5. Global Synthesis of Drought Effects on Food Legume Production.

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    Stefani Daryanto

    Full Text Available Food legume crops play important roles in conservation farming systems and contribute to food security in the developing world. However, in many regions of the world, their production has been adversely affected by drought. Although water scarcity is a severe abiotic constraint of legume crops productivity, it remains unclear how the effects of drought co-vary with legume species, soil texture, agroclimatic region, and drought timing. To address these uncertainties, we collected literature data between 1980 and 2014 that reported monoculture legume yield responses to drought under field conditions, and analyzed this data set using meta-analysis techniques. Our results showed that the amount of water reduction was positively related with yield reduction, but the extent of the impact varied with legume species and the phenological state during which drought occurred. Overall, lentil (Lens culinaris, groundnut (Arachis hypogaea, and pigeon pea (Cajanus cajan were found to experience lower drought-induced yield reduction compared to legumes such as cowpea (Vigna unguiculata and green gram (Vigna radiate. Yield reduction was generally greater when legumes experienced drought during their reproductive stage compared to during their vegetative stage. Legumes grown in soil with medium texture also exhibited greater yield reduction compared to those planted on soil of either coarse or fine texture. In contrast, regions and their associated climatic factors did not significantly affect legume yield reduction. In the face of changing climate, our study provides useful information for agricultural planning and research directions for development of drought-resistant legume species to improve adaptation and resilience of agricultural systems in the drought-prone regions of the world.

  6. Soil Aggregate Features under Tamarindus indica Forest with Different Plant Compositions in Dry-hot Valley%干热河谷不同酸豆林土壤团聚体特征分析

    Institute of Scientific and Technical Information of China (English)

    彭辉; 周红敏; 徐肇友; 瞿虹; 纪中华

    2014-01-01

    Experiments were conducted on the soil aggregates underTamarindus indica forest with bared land(L+G)CK), with fireweed(L+Z), with Paspalum notatum (L+B), withPhyllanthus emblica (L+Y), and withCajanus cajan (L+M) in dry-hot valley of Yunnan. R0.25(percentage of soil aggregates with diameter larger than 0.25mm), mean weight diameter (MWD), geometric mean diameter (GMD) and fractal dimension (D) were determined at 3 soil layers (0 - 10 cm, >10 - 20 cm, >20 – 40 cm). The results showed that L+B promoted the conservation of soil macro-aggregates, L+Z and L+G could improve the soil aggregate structure, L+Y and L+M forestlands reduced the proportion of macro-aggregates in the soil but increased micro-aggregates content. The experiment demonstrated that in dry-hot valley, conservation tillage was conducive to the sustainable management ofT. indica.%采用干筛法对金沙江干热河谷区酸豆林+光板地(裸地)、酸豆林+杂草、酸豆林+百喜草、酸豆林+余甘子、酸豆林+木豆5种酸豆林地在0~10 cm、>10~20 cm、>20~40 cm的3个土层中的R0.25、MWD、GMD和分形维数D变化进行研究,结果表明:5种酸豆林模式中,酸豆林+百喜草模式有利于土壤大团聚体的保存,酸豆林+杂草模式和酸豆林+光板地模式提升土壤团聚结构,但是效果并不显著,而酸豆林+余甘子模式和酸豆林+木豆模式则降低了土壤中大团聚体的比例,使土壤中的微团聚体含量增加;在干热河谷实施保护性耕作有利于酸豆林的可持续经营。

  7. Espaçamentos para produção de sementes de guandu em semeadura tardia

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    GIOMO GERSON SILVA

    2001-01-01

    Full Text Available Avaliaram-se os efeitos dos espaçamentos entre linhas e entre plantas na linha em algumas características das plantas e na produção de sementes de guandu [Cajanus cajan (L. Millsp.], em semeadura tardia (março/1996, em experimento instalado no Núcleo Experimental do Instituto Agronômico, em Campinas (SP. Utilizaram-se dois espaçamentos entre linhas (75 e 100 cm e dois entre plantas na linha (7 e 10 cm, perfazendo quatro tratamentos no delineamento inteiramente casualizado, em esquema fatorial 2 x 2 com três repetições. Avaliaram-se, também, a população final de plantas, altura da planta e de inserção da primeira vagem, diâmetro do caule, número de ramos por planta, produção de vagens e sementes por planta, porcentagem de vagens chochas, contribuição dos ramos na produção da planta e produtividade de sementes. Verificou-se que o diâmetro do caule, o número de ramos primários por planta, a produção de vagens nos ramos e a contribuição destes na produção foram independentemente afetados nas diferentes densidades populacionais, correspondendo aos valores inferiores os espaçamentos menores. A produtividade de sementes, em média 821 kg.ha-1, não foi significativamente afetada nos espaçamentos estudados, do que se conclui que qualquer um deles pode ser utilizado para a produção de sementes de guandu, em condições de semeadura tardia. As plantas apresentaram porte baixo, caule fino e maturação uniforme de vagens, requisitos considerados importantes para a realização de colheita mecanizada de sementes.

  8. Atributos químicos e estabilidade de agregados sob diferentes culturas de cobertura em Latossolo do cerrado Chemical properties and aggregate stability under different cover crops in cerrado Oxisol

    Directory of Open Access Journals (Sweden)

    Glenio G. Santos

    2012-11-01

    Full Text Available Objetivou-se, com este trabalho, avaliar o efeito de diferentes culturas de cobertura sobre os atributos químicos e a estabilidade de agregados de um Latossolo do cerrado, sob plantio direto. O estudo foi conduzido em área experimental na Embrapa Arroz e Feijão, em Santo Antônio de Goiás, GO. As culturas de cobertura avaliadas foram: braquiária, milho em consórcio com braquiária (integração lavoura-pecuária, guandu anão, milheto, capim mombaça, sorgo granífero, estilosantes e crotalária. As amostras foram coletadas em abril de 2005 e 2006. O delineamento experimental foi o de blocos completos ao acaso com quatro repetições e os tratamentos arranjados em esquema fatorial 8 x 2, sendo oito culturas de cobertura e duas profundidades de amostragem do solo: 0-0,10 e 0,10-0,20 m. As culturas de cobertura influenciam, de forma diferenciada, os valores de pH e os teores de cálcio, magnésio, alumínio, fósforo, potássio, cobre, zinco e ferro do solo. O tratamento estilosantes tem maior poder em acidificar o solo. A agregação do solo varia com as culturas de cobertura e com a profundidade.The objective of this study was to evaluate the effect of different cover crops on chemical properties and aggregate stability in a cerrado Oxisol under no-tillage. The study was carried out in Embrapa Rice and Beans, in Santo Antônio de Goiás, GO, Brazil. The cover crops evaluated were: Urochloa brizantha, Urochloa brizantha and corn in association (crop-livestock integrated, Cajanus cajan, Pennisetum glaucum, Panicum maximum, Sorghum bicolor, Stylosanthes guianensis and Crotalaria juncea. The soil samples were collected in April and September 2005 and April 2006. The experimental design was in completely randomized blocks with four replications and treatments arranged in factorial scheme 8 x 2, eight cover crops and two soil sampling depths, 0-0.10 and 0.10-0.20 m. The different cover crops affect pH values and calcium, magnesium, aluminum

  9. 木豆石豆率与栽培地、种皮色及浸泡方法的相关性研究%A Study on the Stiff Seed Ration of Pigeonpea Landrace of Yunnan with Different Coat Color

    Institute of Scientific and Technical Information of China (English)

    张建云; 李正红; 周朝鸿; 惠雅玲; 刘秀贤; 肖银松

    2002-01-01

    @@木豆(Cajanus cajan (L.)Millsp)的石豆是指在浸泡过程中,不能吸水膨胀的木豆。 石豆率的高低,对木豆加工利用及选育有直接影响,如加工成本升高、发芽出土率降低等。近年来,中国林科院资源昆虫所对木豆的用途作了大量的研究工作,已开发出木豆蛋白饲料[1]、香酥木豆[2]、木豆豆沙[3],并开展了超强吸水剂[4~5] 、木豆叶蛋白的提取[6]和木豆贮藏[7]等的研究。在研究过程中发现,木豆的石豆率非常高,一般在40%左右,有的高达60%,造成木豆的利用率降低,生产成本高,不利于木豆的食用开发;对选育而言,由于石豆率的升高,直接导致木豆发芽出土率降低,制约木豆的推广发展。本文就不同栽培地、不同种皮色的地方栽培木豆,分别采用冷水、沸水浸泡不同时间后石豆率的变化进行了研究,探讨木豆与浸泡温度、时间及栽培地、种皮色等的关系。为木豆的推广和食用开发提供理论依据。

  10. 木豆籽实贮存辐射防虫的研究%A Study on the Storage of Pigeon Pea Seed

    Institute of Scientific and Technical Information of China (English)

    张建云; 李正红; 刘秀贤; 周朝鸿; 惠雅玲

    2001-01-01

    @@ 木豆[Cajanus cajan (L.) Millsp]又名三叶豆、鸽子豆, 为蝶形花科(Papilionoideae)木豆属(Cajanus)多年生的常绿灌木,是热带亚热带地区尤其是干热河谷地区荒山造林、退耕还林、改善生态环境的优良先锋树种[1].云南省已把木豆列为长江中上游防护林工程、澜沧江防护林工程、怒江防护林工程及天保工程的造林树种之一.目前中国林业科学研究院资源昆虫研究所已成功开发出木豆蛋白饲料[2]、香酥木豆[3]等用途,但由于木豆籽实在贮存期的主要害虫豆象[Callosobruchs maculates (F.)]危害十分严重(自然存放3~5个月后,至少80%甚至100%的木豆籽实受虫害),约束了木豆的开发利用.长期以来,木豆籽实贮存一直制约着木豆推广和加工的发展,也是面临的一大难题.对木豆籽实的贮存,通常采用化学药剂法如氯氰菊酯等防虫,给木豆的食用开发带来不利影响.据报道,印度采取装入塑料袋在太阳下曝晒,达到贮存目的[4].本实验采用食品工业用蒸煮袋包装,Co 60辐射,同时做一组对照样,采用F测验和新复极差法方差分析[5],并考虑成本因素,筛选出最佳贮存方法,为木豆的推广和食用开发奠定了技术基础. A Study on the Storage of Pigeon Pea Seed

  11. 8种豆科灌木栽培种丛枝菌根真菌种类及分布%The category and distribution of arbuscular mycorrhizal fungi from the rhizosphere of eight cultivated leguminous shrubs

    Institute of Scientific and Technical Information of China (English)

    吴涛; 姚红艳; 莫本田; 龙忠富; 罗充

    2016-01-01

    采集人工栽培决明(Cassia tora)、假木豆(Dendrolobium triangulare)、紫穗槐(Amorpha fruticosa)、白刺花(Sophora davidii)、猪屎豆(Crotalaria pallida)、多花木蓝(lndigofera amblyantha)、木豆(Cajanus cajan)和银合欢(Leucaenaleucocephala)8种豆科灌木植物根际5-10、10-20和20-30 cm 3个土层的土样,研究其根际丛枝菌根(AM)真菌的侵染率、孢子密度、种类及多样性.结果表明,8种豆科灌木植物AM真菌侵染率普遍较高,最高的为猪屎豆,80.8%;土壤速效磷含量与侵染率呈显著负相关(r=-0.733,P=0.039);根际土壤中的AM真菌孢子主要分布于5-20 cm土层,5-10 cm土层猪屎豆孢子密度最大,为115.33个·10 g-1土壤;宿主植物种类与土壤层位对孢子密度交互效应影响显著(F=100.497,P<0.001);AM真菌优势种枫香球囊霉(Glomus liquidambaris)、脆无梗囊霉(Acaulospora delicate)在8种豆科灌木植物的土壤中均有分布.本研究表明,喀斯特地区适生豆科灌木植物具有丰富的AM真菌资源,今后可以在喀斯特地区开展豆科灌木植物接种优势AM真菌和根瘤菌等相关研究,这将对农牧业生产和退化生态系统修复效应的影响具有重要意义.

  12. Influence of host origin on host choice of the parasitoid Dinarmus basalis: does upbringing influence choices later in life?

    Science.gov (United States)

    Sankara, F; Dabiré, L C B; Ilboudo, Z; Dugravot, S; Cortesero, A M; Sanon, A

    2014-01-01

    The aim of this study was to investigate the influence of volatile compounds from four secondary host plants on the ability of Dinarmus basalis Rond. (Hymenoptera: Pteromalidae) to locate, recognize, and parasitize its host, 4(th)instar larvae or pupae of Callosobruchus maculatus F. (Coleoptera: Chrysomelidae). To examine this, strains of D. basalis were transferred from cow-pea seeds (Vigna unguiculata (L.) Walp. (Fabales: Fabaceae)) to pigeon pea (Cajanus cajan (L.) Millsp.) and two varieties of Bambara groundnut (Vigna subterranea (L.) Verdc.) seeds. The ability of D. basalis females to recognize the volatile compounds emanating from their complex host plant was tested by using a Y-tube olfactometer and a three-dimensional device. The results suggest that when females have a choice between pure air and the air emanating from their complex host of origin, they are attracted to the air tainted by the volatile compounds they have become accustomed to. They spent significantly more time (p < 0.0001) in the branch of the tube leading to the odorous air than in the tube leading to the pure air. When females from pigeon pea seed hosts were offered a choice between cowpea and pigeon pea seeds, all containing 4(th)instar larvae, the familiar odor of pigeon pea seeds were most attractive. When females from Bambara groundnut (white and striped) seed hosts were offered a choice between cowpea and pigeon pea seeds, all containing 4(th)instar larvae, they were significantly attracted to the odour of cowpea seeds. In the three-dimensional system, the females from the four strains did not appear to have any preference for a given type of seed containing 4(th)instar larvae or pupae. The parasitism rate remained high on all four types of seeds used. These results show that the use of D. basalis as a biological control agent is possible in host changing situations where C. maculatus starts to attack other legumes. The results of this study also provide information supporting the

  13. Produção e estado nutricional do milho em cultivo intercalar com adubos verdes

    Directory of Open Access Journals (Sweden)

    R. Heinrichs

    2002-03-01

    Full Text Available A utilização da adubação verde para estabelecer a diversidade e o equilíbrio do sistema de produção é um dos paradigmas da agricultura moderna. Com o objetivo de avaliar a produção e o estado nutricional da cultura de milho sob cultivo intercalar com adubos verdes, foi realizado, entre 1995 e 1997, um experimento em campo, em Piracicaba (SP, em um Litossolo Vermelho eutrófico. O milho foi semeado no espaçamento de 0,90m nas entrelinhas, perfazendo aproximadamente 50.000plantas por hectare. Os tratamentos constaram de uma testemunha, sem cultivo intercalar, e quatro espécies de adubos verdes: mucuna anã (Mucuna deeringiana (Bort. Merr, guandu anão (Cajanus cajan (L. Millsp, crotalária (Crotalaria spectabilis Roth e feijão-de-porco (Canavalia ensiformis (L. DC., semeados sem adubação, no meio da entrelinha, distante 45cm da linha do milho, em duas épocas: simultânea à semeadura do milho e 30dias após. O solo, no primeiro ano, foi preparado sob sistema convencional e, no segundo, cultivaram-se as culturas sob semeadura direta. O delineamento experimental foi de blocos ao acaso com parcelas subdivididas e quatro repetições. O estado nutricional e a produção de grãos do milho apresentaram melhores resultados no cultivo consorciado com feijão-de-porco. Os efeitos positivos desta espécie na produtividade de grãos de milho foram mais acentuados no segundo ano de adoção do cultivo consorciado, quando o sistema de manejo do solo foi semeadura direta. A semeadura dos adubos verdes simultânea ao milho foi o manejo mais recomendável, por não prejudicar o desenvolvimento do milho e reduzir a operação pós-plantio.

  14. Plants screening for roadside slope protection by mixture seeding in Yichang-Changyang section of Shanghai-Chengdu freeway%沪蓉西高速公路宜昌-长阳段混播护坡植物筛选

    Institute of Scientific and Technical Information of China (English)

    陈学平; 张洪江; 张翔; 江玉林; 李海芬; 曹传林; 王金娟

    2011-01-01

    Three indices including early growth ability, stress resistance, growth potential were used to evaluate 19 tree plants and 8 herbaceous plants based upon introduce and observation of the experiment plants in Yichang-Changyang section of Shanghai-Chengdu freeway in Hubei Province.Value of subjection was calculated based on classifying analysis, ranking series and comprehensive assessment.Results show that the lndigofera pseudotinctoria have the best performance, Solanum deflexicarpum,Desmodium sinuatum, Cynodon dactylon, Melia azedarach, Robinia pseudoacacia, Lespedeza cunecta,Virex negundo, Paspalum notatum rank the second, Festuca arundinacea, Eragrostis curvula, Cudrania tricuspidata, Rhus chinensis, Dodonaea viscosa, Senna obtusifolia, Rhamnus utilis, Ailanthus altissima rank the third, Lotus corniculatus, Pterocarya kunth, Kummerrowia stipulacea, Sapiu sebiferum,Eremochloa ophiuroides, Trifolium repens rank the forth, Pinus massoniana rank the poorest; Cajanus cajan, Tephrosia candid and Crotaria assamica grew fast in the seeding year, but nearly all failed survive the winter.%在沪蓉西高速公路宜昌-长阳段引种植物观测的基础上,选择初期生长力、抗逆性、生长势3个指标评价19种木本植物、8种草本植物的表现,并以隶属度值为基础进行3个指标的分类排序与等级划分及综合评价.结果表明:供试植物中以马棘表现最优,苦刺、波叶山蚂蟥、狗牙根、苦楝、刺槐、截叶胡枝子、荆条、百喜草表现良好,高羊茅、弯叶画眉草、柘树、盐肤木、坡柳、决明、冻绿、臭椿表现中等,百脉根、枫杨、鸡眼草、乌桕、假俭草、白三叶等表现较差,马尾松表现最差;木豆、山毛豆、猪屎豆第1年生长速度十分迅速,但抗寒性差,持续能力差.

  15. Análise econômica do uso de leguminosas estivais intercalares à cultura do milho, na ausência e na presença de adubação nitrogenada, no oeste de Santa Catarina

    Directory of Open Access Journals (Sweden)

    E. Spagnollo

    2001-09-01

    Full Text Available O uso de leguminosas constitui boa alternativa para suprir N e aumentar o rendimento das culturas comerciais. A aceitação desta prática por agricultores e extensionistas, entretanto, depende da demonstração de sua vantagem econômica em relação ao sistema tradicional. Neste estudo, avaliou-se o efeito do cultivo intercalar de quatro leguminosas estivais de cobertura do solo [feijão de porco (Canavalia ensiformis L., guandu (Cajanus cajan L. cv. roxo anão, mucuna cinza (Stizolobium niveum Kuntze e soja preta (Glycine sp] sobre a receita líquida da cultura do milho, em três doses de N (0, 60 e 120 kg ha-1, comparativamente ao sistema tradicional de milho exclusivamente (testemunha. Dois experimentos foram realizados, durante quatro e cinco anos, respectivamente, em sistemas de preparo convencional (PC e preparo reduzido (PR, num Latossolo Vermelho distroférrico, em Chapecó (SC, sendo os resultados apresentados na média das safras. A cultura do milho, sem leguminosa e sem adubação nitrogenada, apresentou uma receita líquida de 45 R$ ha-1 no PC e de 72 R$ ha-1 no PR. O cultivo intercalar das leguminosas promoveu incrementos na receita líquida da cultura do milho que variaram de 64 a 312 R$ ha-1 (142 a 693%, no PC, e de 57 a 147 R$ ha-1 (79 a 204%, no PR, na ausência de adubação nitrogenada. Mesmo com aplicação de N, as leguminosas promoveram aumento na receita líquida da cultura do milho, porém de menor magnitude. O cultivo intercalar de leguminosas estivais de cobertura do solo demonstrou-se uma alternativa viável para aumentar a receita líquida da cultura do milho. Os benefícios econômicos obtidos com esta prática provavelmente compensam o aumento em mão-de-obra.

  16. Genetic Diversity Analysis of Pigeonpea Germplasm Resources by AFLP%木豆种质资源AFLP标记遗传多样性分析

    Institute of Scientific and Technical Information of China (English)

    闫龙; 关建平; 宗绪晓

    2007-01-01

    利用AFLP分子标记技术,以木豆野生种(Cajanus scarabaeiodes)为对照,对来自印度、中国、非洲和美洲的139份栽培木豆(Cajanus cajan)种质遗传多样性进行研究.17对引物共扩增出502条清晰可辨的带,其中多态性带494条.遗传相似性系数UPGMA聚类图,揭示木豆种内存在足够的遗传多样性,可用于种质资源的准确鉴别与分类.借助遗传距离的ME进化树又将参试种质分成8个组群.分析结果表明,印度地方资源遗传多样性广泛,育成品种遗传基础广阔;非洲、美洲资源遗传多样性较丰富,且与印度资源的亲缘关系较密切.中国地方资源遗传多样性独特,且与印度、非洲、美洲栽培木豆资源亲缘关系不明显.研究结果基本支持印度木豆起源和多样性中心、非洲次生多样性中心的观点,并提出中国次生起源中心和遗传多样性中心假说.研究还发现,木豆核质互作雄性不育系(A)和保持系(B)间存在独特的AFLP谱带差异,可能有助于分子标记辅助的木豆杂种优势利用和杂交种培育.

  17. Experimental Research on Seedling Greening Plants on Rock Slope Growth Rhythm of Common of Expressway in Guizhou%贵州高速公路岩石边坡常用绿化植物幼苗生长规律实验研究

    Institute of Scientific and Technical Information of China (English)

    符治明; 张晓龙; 李洪青; 唐洋洋

    2012-01-01

    10 species of trees, shrubs and 10 herbs, which are commonly used as greening plants on rocky slopes of expressway in Guihzou, were studied for their seedling growth rhythm, in order to select suitable plant species for highway greening. The results showed that: 1)Cassia bicapsularis is the first emerging species, and Cajanus cajan, Sophora xanthantha and Amorpha fruticosa have higher germination rate and height; 2) the height growth of various tree and shrub seedlings presented a "slow-fast-slow" , namely "S" type, of growth rhythms;3) the herb plants, Cynodon dactylon and Trifolium repens, have higher coverage rate and height growth; 4)Robinia pseudoacacia, Sophora xanthantho, Indigofera pseudotinctoria, Cynodondactylon, Trifolium repens, Festuca arundinacea,and Lolium perenne can be used as the pioneer plant species, which have great interspecific competition advantageous, thereby speed up the formation of plant communities and achieve the goals of rapidly covering and fixation of the highway rock slopes.%本文通过播种试验,研究贵州高速公路边坡绿化常用的10种乔、灌植物和10种草本植物的幼苗生长规律,以选择出最适合高速公路绿化的植物种类。结果表明:乔灌木植物中双荚决明出苗时间最短,木豆、双荚决明、紫穗槐、多花木兰的出苗率及高生长较大,各种乔灌木幼苗的高生长总体呈现“慢一快一慢”即“S”型的生长节律;草本植物中狗牙根和自三叶的盖度及高生长较大;刺槐、双荚决明和多花木兰及狗牙根、白三叶、草坪高羊茅、草坪黑麦草等可作为主要的护坡植物先锋种,其应用于高速公路岩石边坡绿化可在种问竞争中处于相对有利的地位,有利于植物群落的形成和实现快速覆盖固坡的作用。

  18. Coberturas vegetais, doses de nitrogênio e inoculação de sementes com Azospirillum brasilense em arroz de terras altas no sistema plantio direto

    Directory of Open Access Journals (Sweden)

    Douglas de Castilho Gitti

    2012-01-01

    Full Text Available O arroz é importante fonte de energia e proteínas para a população mundial, principalmente na Ásia e Oceania. No Brasil, juntamente com o feijão, constitui a base da alimentação. Tecnologias sustentáveis que reduzam custos da produção e aumentem a produtividade do arroz podem garantir seu suprimento em períodos de alta demanda. O objetivo deste trabalho foi avaliar diferentes coberturas vegetais antecessoras (milheto [Pennisetum americanum], crotalária [Crotalaria juncea], guandu [Cajanus cajan], braquiária [Brachiaria ruziziensis], milheto + crotalária e milheto + guandu, doses de nitrogênio (N em cobertura (0, 40, 80 e 120 kg ha-1 e o efeito da inoculação de sementes com Azospirillum brasilense no arroz de terras altas em sistema plantio direto no desenvolvimento e na produtividade. Utilizou-se o delineamento experimental em blocos casualizados em esquema fatorial 6x4x2 com quatro repetições. O estudo foi desenvolvido em um Latossolo Vermelho, em Selvíria, Estado do Mato Grosso do Sul, Brasil, em 2011/2012. O cultivo do guandu antecedendo o arroz proporcionou maior produtividade do arroz somente em comparação a B. ruziziensi. A produtividade do arroz em função das doses de N em cobertura se ajustou a uma função quadrática. Não houve influência da inoculação de sementes com A. brasilense sobre a produtividade do arroz, porém houve interação entre a inoculação e as coberturas vegetais sobre o teor de N foliar, número de panículas por m², matéria seca de plantas de arroz e a massa de cem grãos.

  19. Advances in genetics and molecular breeding of three legume crops of semi-arid tropics using next-generation sequencing and high-throughput genotyping technologies

    Indian Academy of Sciences (India)

    Rajeev K Varshney; Himabindu Kudapa; Manish Roorkiwal; Mahendar Thudi; Manish K Pandey; Rachit K Saxena; Siva K Chamarthi; Murali Mohan S; Nalini Mallikarjuna; Hari Upadhyaya; Pooran M Gaur; L Krishnamurthy; K B Saxena; Shyam N Nigam; Suresh Pande

    2012-11-01

    Molecular markers are the most powerful genomic tools to increase the efficiency and precision of breeding practices for crop improvement. Progress in the development of genomic resources in the leading legume crops of the semi-arid tropics (SAT), namely, chickpea (Cicer arietinum), pigeonpea (Cajanus cajan) and groundnut (Arachis hypogaea), as compared to other crop species like cereals, has been very slow. With the advances in next-generation sequencing (NGS) and high-throughput (HTP) genotyping methods, there is a shift in development of genomic resources including molecular markers in these crops. For instance, 2,000 to 3,000 novel simple sequence repeats (SSR) markers have been developed each for chickpea, pigeonpea and groundnut. Based on Sanger, 454/FLX and Illumina transcript reads, transcriptome assemblies have been developed for chickpea (44,845 transcript assembly contigs, or TACs) and pigeonpea (21,434 TACs). Illumina sequencing of some parental genotypes of mapping populations has resulted in the development of 120 million reads for chickpea and 128.9 million reads for pigeonpea. Alignment of these Illumina reads with respective transcriptome assemblies have provided > 10,000 SNPs each in chickpea and pigeonpea. A variety of SNP genotyping platforms including GoldenGate, VeraCode and Competitive Allele Specific PCR (KASPar) assays have been developed in chickpea and pigeonpea. By using above resources, the first-generation or comprehensive genetic maps have been developed in the three legume speciesmentioned above. Analysis of phenotyping data together with genotyping data has provided candidate markers for drought-tolerance-related root traits in chickpea, resistance to foliar diseases in groundnut and sterility mosaic disease (SMD) and fertility restoration in pigeonpea. Together with these trait-associated markers along with those already available, molecular breeding programmes have been initiated for enhancing drought tolerance, resistance to

  20. Nutritional characteristics of two pigeon pea hybrids – Liming and phosphated fertilization

    Directory of Open Access Journals (Sweden)

    Marcia Atauri Cardelli de Lucena

    2012-12-01

    Full Text Available The use of legumes in animal production systems can be a sustainable alternative as a protein source in rotational grazing system and/or as a protein bank. Pigeon pea (Cajanus cajan (L. Millsp. figure as an example of success of this use on animal nutrition. The development of this species can be limited by the high acidity and low soil phosphorus content. There is a lack of scientific information on the effects of liming and phosphorus fertilization on some nutritional variables of two pigeon pea new hybrids. This study was conducted in pots containing 5 kg of soil in a greenhouse at the Instituto de Zootecnia, Nova Odessa, São Paulo State. There were studied two pigeon pea hybrids, H1 and H2, and the treatments involved agronomic practices: 1 No liming and without phosphorus (control, 2 Liming (L, 3 Phosphorus fertilization (P and 4 Liming plus phosphorus. Liming was proposed to increase soil base saturation to 50%, it was used dolomite lime PRNT = 90%, in an amount corresponding to 4.5 t/ha. Phosphorus fertilization (as superphosphate rate was 60 kg/ha of PO25. The experimental units were allocated according to a complete randomised block design, with five replications. We analyzed the levels of crude protein (CP, neutral detergent fiber (NDF, acid detergent fiber (ADF, in pigeon pea shoot at 45 days of age. Statistical analyzes were performed using the software SISVAR, averages were compared using test for multiple comparisons Student Newman-Keuls - SNK test (P < 0.05. The H1 hybrid had the highest content of CP, by applying P, lime plus P and the control treatment compared to H2 hybrid. The association lime plus P resulted in higher content of CP mainly due to the increased availability of P for plants. Smaller values were observed for NDF in H2 with P application. Lower values of ADF were observed in H1 in both control treatment and P application. The ADF values were lower for the hybrid H2 only for the treatment lime plus P. The two

  1. Rendimento do feijoeiro influenciado por sistemas de manejo em um Latossolo Vermelho de cerrado = Influence of management systems in Oxisol on common bean yield

    Directory of Open Access Journals (Sweden)

    Luis Gustavo Akihiro Sanches Suzuki

    2007-01-01

    Full Text Available Objetivou-se avaliar a influência de sistemas de manejo no rendimento de grãos de feijão em um Latossolo Vermelho de cerrado. A pesquisa iniciou-se em 1997 e a avaliação do rendimento de grãos foi realizada no inverno de 2002. Utilizou-se o delineamento experimental de blocos ao acaso no esquema em faixas com parcelas subsubdivididas. Os tratamentos foram constituídos nas faixas pela combinação de 5 plantasde cobertura (Mucuna aterrima, Pennisetum americanum, Crotalaria juncea, Cajanus cajan e pousio; no outro sentido, as faixas foram subdivididas e constituídas pelo sistema de semeadura direta e convencional (preparo do solo com grade aradora e grade niveladora, as quais, por sua vez, foram subsubdivididas com as culturas de verão milho, soja e algodão. Concluiu-se que as plantas de cobertura, após cinco anos, não influenciaram o rendimento de grãos; a massa de 100 grãos de feijão foi maior na semeadura convencional, porém, orendimento não diferiu entre os preparos; na semeadura convencional, a sucessão com algodão, nos dois primeiros anos agrícolas, proporcionou grãos de feijão mais pesados e maior rendimento; na semeadura direta, a sucessão que incluía algodão ou soja nos doisprimeiros anos agrícolas proporcionou maiores rendimentos ao feijoeiro.This study aimed to evaluate the influence of management on common bean in Oxisol. The research began in 1997 and the yield evaluation was carried out in the winter of 2002. The experimental design was randomized blocks with split split-plot design. The treatments were constituted by the combination of five cover plants, two soil tillages and three crop successions. It was concluded that cover plants, after five years, did not influence the common bean; the 100-grain weight of bean was higher in conventional tillage, but theyield did not show any difference between the tillages; in conventional tillage, cotton crop sucession in the first two years provided larger common

  2. Caracterização genética de rizóbios nativos dos tabuleiros costeiros eficientes em culturas do guandu e caupi

    Directory of Open Access Journals (Sweden)

    Fernandes Marcelo Ferreira

    2003-01-01

    Full Text Available O objetivo deste trabalho foi caracterizar geneticamente sete estirpes de rizóbios nativos dos tabuleiros costeiros de Sergipe com alta eficiência de fixação biológica do N2 em associação com guandu (Cajanus cajan e caupi (Vigna unguiculata. A amplificação do DNA pela técnica de PCR (polymerase chain reaction com o oligonucleotídeo específico BOX indicou um grau elevado de diversidade genética, uma vez que todas as estirpes apresentaram perfis únicos de DNA. A análise por BOX-PCR revelou, ainda, que essa metodologia é eficiente para diferenciar estirpes, mas não para a diferenciação de espécies de rizóbio. Pela técnica do RFLP (restriction fragment length polymorphism da região do DNA que codifica o gene 16S rRNA e da região intergênica entre os genes 16S e 23S rRNA, com cinco enzimas de restrição, bem como pelo seqüenciamento parcial da região do 16S rRNA, foi possível classificar as estirpes nos gêneros Bradyrhizobium e Rhizobium. Houve coerência entre as análises envolvendo a região do 16S rRNA, mas o agrupamento com uma das estirpes diferiu pela análise do espaço intergênico. Os resultados obtidos com a estirpe R11 indicam variabilidade genética elevada em relação às espécies de rizóbios descritas, inclusive diferindo em diversas bases da região do 16S rRNA, e podem indicar uma nova espécie.

  3. Advances in genetics and molecular breeding of three legume crops of semi-arid tropics using next-generation sequencing and high-throughput genotyping technologies.

    Science.gov (United States)

    Varshney, Rajeev K; Kudapa, Himabindu; Roorkiwal, Manish; Thudi, Mahendar; Pandey, Manish K; Saxena, Rachit K; Chamarthi, Siva K; Mohan, S Murali; Mallikarjuna, Nalini; Upadhyaya, Hari; Gaur, Pooran M; Krishnamurthy, L; Saxena, K B; Nigam, Shyam N; Pande, Suresh

    2012-11-01

    Molecular markers are the most powerful genomic tools to increase the efficiency and precision of breeding practices for crop improvement. Progress in the development of genomic resources in the leading legume crops of the semi-arid tropics (SAT), namely, chickpea (Cicer arietinum), pigeonpea (Cajanus cajan) and groundnut (Arachis hypogaea), as compared to other crop species like cereals, has been very slow. With the advances in next-generation sequencing (NGS) and high-throughput (HTP) genotyping methods, there is a shift in development of genomic resources including molecular markers in these crops. For instance, 2,000 to 3,000 novel simple sequence repeats (SSR) markers have been developed each for chickpea, pigeonpea and groundnut. Based on Sanger, 454/FLX and Illumina transcript reads, transcriptome assemblies have been developed for chickpea (44,845 transcript assembly contigs, or TACs) and pigeonpea (21,434 TACs). Illumina sequencing of some parental genotypes of mapping populations has resulted in the development of 120 million reads for chickpea and 128.9 million reads for pigeonpea. Alignment of these Illumina reads with respective transcriptome assemblies have provided more than 10,000 SNPs each in chickpea and pigeonpea. A variety of SNP genotyping platforms including GoldenGate, VeraCode and Competitive Allele Specific PCR (KASPar) assays have been developed in chickpea and pigeonpea. By using above resources, the first-generation or comprehensive genetic maps have been developed in the three legume speciesmentioned above. Analysis of phenotyping data together with genotyping data has provided candidate markers for drought-tolerance-related root traits in chickpea, resistance to foliar diseases in groundnut and sterility mosaic disease (SMD) and fertility restoration in pigeonpea. Together with these traitassociated markers along with those already available, molecular breeding programmes have been initiated for enhancing drought tolerance, resistance

  4. Effect of cropping systems in no-till farming on the quality of a Brazilian Oxisol

    Directory of Open Access Journals (Sweden)

    Getulio de Freitas Seben Junior

    2014-08-01

    Full Text Available The no-till system with complex cropping sequences may improve the structural quality and carbon (C sequestration in soils of the tropics. Thus, the objective of this study was to evaluate the effects of cropping sequences after eight years under the no-till system on the physical properties and C sequestration in an Oxisol in the municipality of Jaboticabal, Sao Paulo, Brazil. A randomized split-block design with three replications was used. The treatments were combinations of three summer cropping sequences - corn/corn (Zea mays L. (CC, soybean/soybean (Glycine max L. Merryll (SS, and soybean-corn (SC; and seven winter crops - corn, sunflower (Helianthus annuus L., oilseed radish (Raphanus sativus L., pearl millet (Pennisetum americanum (L. Leeke, pigeon pea (Cajanus cajan (L. Millsp, grain sorghum (Sorghum bicolor (L. Moench, and sunn hemp (Crotalaria juncea L.. Soil samples were taken at the 0-10 cm depth after eight years of experimentation. Soil under SC and CC had higher mean weight diameter (3.63 and 3.55 mm, respectively and geometric mean diameter (3.55 and 2.92 mm of the aggregates compared to soil under SS (3.18 and 2.46 mm. The CC resulted in the highest soil organic C content (17.07 g kg-1, soil C stock (15.70 Mg ha-1, and rate of C sequestration (0.70 Mg ha-1 yr-1 among the summer crops. Among the winter crops, soil under pigeon pea had the highest total porosity (0.50 m³ m-3, and that under sunn hemp had the highest water stable aggregates (93.74 %. In addition, sunn hemp did not differ from grain sorghum and contained the highest soil organic C content (16.82 g kg-1 and also had the highest rate of C sequestration (0.67 Mg ha-1 yr-1. The soil resistance to penetration was the lower limit of the least limiting water range, while the upper limit was air-filled porosity for soil bulk densities higher than 1.39 kg dm-3 for all cropping sequences. Within the SC sequence, soil under corn and pigeon pea increased least limiting water

  5. Legumes and forage species sole or intercropped with corn in soybean-corn succession in midwestern Brazil Espécies leguminosas e forrageiras, solteiras ou consorciadas com milho, na sucessão soja-milho no centro-oeste do Brasil

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    Gessí Ceccon

    2013-02-01

    Full Text Available The feasibility of no-tillage in the Cerrado (Savanna-like vegetation of Brazil depends on the production of sufficient above-ground crop residue, which can be increased by corn-forage intercropping. This study evaluated how above-ground crop residue production and yields of soybean and late-season corn in a soybean-corn rotation were influenced by the following crops in the year before soybean: corn (Zea mays L. intercropped with Brachiaria (Urochloa brizantha cv. Marandu, B. decumbens cv. Basilisk, B. ruziziensis, cv. comum., Panicummaximum cv. Tanzânia, sunn hemp (Crotalaria juncea L., pigeon pea [Cajanus cajan (L. Millsp]; sole corn, forage sorghum [Sorghum bicolor (L. Moench (cv. Santa Elisa], and ruzi grass. In March 2005, corn and forage species were planted in alternate rows spaced 0.90 m apart, and sole forage species were planted in rows spaced 0.45 m apart. In October 2005, the forages were killed with glyphosate and soybean was planted. After the soybean harvest in March 2006, sole late-season corn was planted in the entire experimental area. Corn grain and stover yields were unaffected by intercropping. Above-ground crop residue was greater when corn was intercropped with Tanzania grass (10.7 Mg ha-1, Marandu (10.1 Mg ha-1, and Ruzi Grass (9.8 Mg ha-1 than when corn was not intercropped (4.0 Mg ha-1. The intercropped treatments increased the percentage of soil surface covered with crop residue. Soybean and corn grain yields were higher after sole ruzi grass and intercropped ruzi grass than after other crops. The intercropping corn with Brachiaria spp. and corn with Panicum spp. increases above-ground crop residue production and maintains nutrients in the soil without reducing late-season corn yield and the viability of no-till in the midwestern region of Brazil.A viabilidade do plantio direto no Cerrado brasileiro depende da produção adequada de palha das culturas, que pode ser aumentada pela consorciação de milho (Zea mays L

  6. In situ caecal degradation of roughages in horses Degradação cecal in situ de alimentos volumosos em equinos

    Directory of Open Access Journals (Sweden)

    Vinícius Pimentel Silva

    2010-02-01

    Full Text Available The present study was carried out to evaluate the in situ degradation of dry matter (DM, neutral detergent fiber (NDF and crude protein (CP in roughages by the in situ caecal digestion technique in horses. The roughages evaluated were: Lucerne hay (Medicago sativa, peanut (Arachis pintoi cv. Amarillo, desmodio(Desmodium ovalifolium, stylo (Stylosanthes guianensis cv. Mineirão, pigeon pea (Cajanus cajan, lime-yellow pea (Macrotyloma axillare and coastcross hay (Cynodon dactylon cv. coastcross. The assay was conducted in a complete randomized design with seven roughages and three replications. One mare with a cannula fitted in the caecum was used, fed diet consisting of coastcross hay (80% and concentrate (20% at 2.0% BW, four times a day. Nylon 6.5 × 20 cm bags were used with 45 μ/pore, containing 5.2 g DM/bag, inserting 3 or 4 bags in the caecum at the times of 2, 4, 6, 8, 12, 24 and 48 hours incubation. The caecum in situ degradability parameters of nutrients were obtained by Ørskov model. The DM degradability parameters of all the roughages were significant. There was no fit to the model for pigeon pea for CP and NDF and desmodio. Peanut, stylo and lime-yellow pea presented larger potentially degradable DM with values of 53, 46.5 and 40%, respectively, and higher values for the soluble fraction of 20, 21, 28.6%, with high degradability rates of 10.36, 20.26 and 14.8% h-1. Higher NDF degradation rates were observed in these foodstuffs with values of 9.1 and 11.3, 11.2% h-1, high potentially degradable fraction with values of 55, 51.8 and 47.2%, and greater CP degradation at 48 hours with values of 87, 95, and 94.8%. Peanut, stylo and lime-yellow pea presented potential for use in horses diets.Objetivou-se avaliar a degradação da matéria seca (MS, fibra em detergente neutro (FDN e proteína bruta de alimentos volumosos pela técnica da digestão cecal in situ em equinos. Avaliaram-se as forrageiras: feno de alfafa (Medicago sativa

  7. Comparison of carbon and nitrogen determination methods for samples of a Paleudult subjected to no-till cropping systems Comparação de métodos de determinação de carbono e nitrogênio em amostras de Argissolo sob plantio direto

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    Jeferson Dieckow

    2007-10-01

    Full Text Available Organic carbon (C concentration evaluated by the Walkley-Black method, and total nitrogen(Nconcentration determined by Kjeldahl method, were compared with corresponding results of C and N concentrations obtained through the dry combustion method (Elementar Vario EL analyzer, using samples of ten soil layers down to the depth of 107.5 cm of a kaolinitic Typic Paleudult (220-418 g clay kg-1 along the profile subjected to no-till cropping systems [fallow bare soil, pigeon pea (Cajanus cajan L. Millsp. plus maize, and lablab (Lablab purpureum L. Sweet plus maize]. A close correlation (R² > 0.96 was observed between the C results of the Walkley-Black and dry combustion methods, but a correction was suggested to be applied to C results of Walkley-Black (C = 1.05C W.Black + 0.47. A close correlation (R² > 0.96 was also found between results of Kjeldahl-N and dry combustion-N, but no correction was necessary to be applied. The relationships between results of Walkley-Black and dry combustion and between results of Kjeldahl and dry combustion did not change among soil samples from different management systems, in disagreement to findings of a previous study where results of analytical C recovery were influenced by samples from different managements, supposedly because changes in lability or recalcitrance of organic matter might have affected the wet combustion reactions. A poor correlation (R² = 0.42 was found between the C:N ratios based on Walkley-Black and Kjeldahl analysis and the C:N ratios based on dry combustion analysis. The methods for C and N determination did not affect the interpretation of the effects of management systems on soil C and N stocks.Resultados de concentração de carbono (C, obtidos pelo método Walkley-Black, e resultados de concentração de nitrogênio (N total, determinados pelo método Kjeldahl, foram comparados com os correspondentes resultados de C e N obtidos pelo método de combustão seca (analisador Elementar

  8. Avaliação de coberturas mortas em cultura de alface sob manejo orgânico Evaluation of mulches on organically grown lettuce

    Directory of Open Access Journals (Sweden)

    Fabio F de Oliveira

    2008-06-01

    Full Text Available A cobertura morta do solo com leguminosas e gramíneas é uma prática cultural que traz benefícios aos sistemas de produção. Foi conduzido um experimento no município de Seropédica, estado do Rio de Janeiro, com o objetivo de avaliar o efeito de diferentes tipos de cobertura morta sobre a reinfestação de ervas espontâneas e sobre o desempenho agronômico de alface, em cultivo orgânico. O delineamento experimental adotado foi blocos casualizados com quatro repetições e parcelas de oito plantas na área útil. Os tratamentos foram: bagaço de cana-de-açúcar (Saccharum sp., bambu (Bambuza sp., capim Cameroon (Penisetum purpureum, crotalária (Crotalaria juncea, eritrina (Erythrina poeppigiana, gliricídia (Gliricidia sepium, guandu (Cajanus cajan, mucuna cinza (Mucuna pruriens e controle (sem cobertura dos canteiros. Estimou-se a decomposição in situ e a liberação de nitrogênio de cada cobertura morta. Foram avaliados dois ciclos consecutivos de alface, cultivar Regina, na mesma área, visando a comparar o efeito residual das coberturas mortas. Observou-se maior acúmulo de N nos resíduos vegetais das leguminosas (máximo de 1.010 kg ha-1 com mucuna-cinza. Em relação à decomposição da cobertura morta, avaliada na colheita do primeiro ciclo de alface (35 dias após o transplante, as leguminosas apresentaram percentuais inferiores aos das gramíneas de massa seca e N remanescentes. A reinfestação dos canteiros pelas ervas espontâneas não diferiu significativamente entre coberturas mortas, variando entre 31 e 58 plantas m-2, mas a redução da densidade populacional da vegetação reinfestante chegou a 83% em comparação ao tratamento controle. Em ambos os ciclos de cultivo da alface, a massa fresca (de 315,8 a 366,0 e de 202,9 a 225,0 g planta-1, respectivamente nos primeiro e segundo ciclos, o diâmetro da cabeça (de 30,8 a 31,7 e de 25,5 a 28,5 cm e o teor de N (de 32,3 a 38,8 e de 28,0 a 30,3 g kg-1 foram superiores

  9. Management of crotalaria and pigeon pea for control of yam nematode diseases Manejo da crotalária e do guandu no controle de nematoses do inhame

    Directory of Open Access Journals (Sweden)

    Marlon da Silva Garrido

    2008-09-01

    Full Text Available Management of plant-parasitic nematodes with the use of nematicides has not been recommended for small farmers that grow yam in the Northeastern region of Brazil, due to its high cost and residue toxicity. The use of plants with antagonistic effect to nematodes and green manure which improves soil chemical, physical and biological characteristics can be a viable and low cost alternative to control parasitic nematodes. This work aimed to evaluate the effect of crotalaria (Crotalaria juncea and pigeon pea (Cajanus cajan plants on the control of yam nematodes. Three experiments were carried out. The first was conducted under in vitro conditions to evaluate the nematostatic and nematicide effect of extracts from fresh and dry matter of the above ground parts of crotalaria, pigeon pea, and the combination of both. The second experiment was carried out under greenhouse conditions to evaluate the effect of soil amendment with crotalaria, pigeon pea, and the combination of both in the infectivity of Scutellonema bradys, using tomato plants as the host plant. The third experiment was conducted under field conditions to evaluate the effect of crotalaria, pigeon pea, and the combination of both, cultivated between yam planting rows and incorporated to soil surface, on yam nematodes. The aqueous extract obtained form fresh matter of crotalaria had a nematicide effect of 100% for S. bradys. Extracts from dry matter of both crotalaria and pigeon pea did not have any nematicide effect, but had a nematostatic effect. Incorporation of crotalaria to soil inhibited infectivity of S. bradys in tomato seedlings. These results showed that planting crotalaria alone or in combination with pigeon pea, between the yam planting rows, is an efficient method for controlling S. bradys and Rotylenchulus reniformis associated with yams. Crotalaria can be used for controlling these plant-parasitic nematodes in soil.O manejo de fitomenatóides com o uso de nematicidas não tem

  10. Crescimento aéreo e radicular da soja e de plantas de cobertura em camadas compactadas de solo Shoot and root growth of soybean and cover crops as affected by soil compaction

    Directory of Open Access Journals (Sweden)

    José Salvador Simoneti Foloni

    2006-02-01

    Full Text Available O cultivo de plantas de cobertura com sistema radicular vigoroso em rotação de culturas pode melhorar a qualidade física de solos compactados. O objetivo deste trabalho foi avaliar o crescimento aéreo e radicular da soja e de cinco espécies utilizadas como plantas de cobertura de verão (guandu, guandu anão, mucuna preta, labe labe e crotalária juncea, em solo submetido a três níveis de compactação em subsuperfíce. Utilizou-se um Nitossolo Vermelho argiloso em vasos montados com anéis de PVC sobrepostos, com diâmetro interno de 20 cm e altura de 50 cm. A camada compactada localizada de 20-30 cm de profundidade foi caracterizada pelas densidades do solo de 1,13, 1,34 e 1,56 g cm-3 e 0,26, 0,66 e 1,98 MPa de impedância mecânica, respectivamente. Aos 60 dias da emergência das plantas, determinaram-se as massas da matéria seca da parte aérea e das raízes, a densidade do comprimento radicular e o diâmetro médio radicular. Apesar de alterar a distribuição do sistema radicular ao longo do perfil do solo, o impedimento físico em subsuperfície não diminuiu a produção total de raízes da soja e dos adubos verdes, com exceção da crotalária juncea. A mucuna preta foi a leguminosa mais tolerante e a soja a mais sensível à compactação do solo. O sistema radicular da crotalária juncea apresentou potencial de formação de "bioporos" compatível ao da mucuna preta, apesar de ter sido relativamente mais sensível à compactação do solo. O diâmetro médio das raízes da soja e da crotalária juncea apresentou correlação significativa com o crescimento radicular dentro da camada de solo compactado.The cultivation of cover crops with vigorous root systems in crop rotation can improve the physical quality of compacted soils. The objective of this study was to evaluate shoot and root growth of soybean (Glycine max and five species used as summer cover crops (Cajanus cajan, Cajanus cajan var. small, Mucuna aterrima, Dolichos

  11. Soil and Water Conservation Function of Composite Shrub Grasslands on the Hot Arid Valley in Yunnan Province%干热河谷区灌草草地的水土保持效应

    Institute of Scientific and Technical Information of China (English)

    毕玉芬; 车伟光; 许岳飞

    2009-01-01

    为了恢复干热河谷退化山区草地的生态环境.提高草地的水土保持能力,于2004~2007年在金沙江干热河谷区的云南省永胜县设置样点.在退化严重的草地上建植了7个组合模式的灌草复合型草地,以凋落物量、最大持水量、有效截流量、草地盖度、草地土壤崩解时间为指标.对建植3年的灌草草地的水保效果进行研究.结果表明."灌+草+藤"最适宜金沙江干热河谷区的植被重建.其中"木豆+非洲狗尾草+高羊茅+大翼豆"建植模式显著地提高草地植被盖度和凋落物重量.凋落物的持水能力与凋落物重量呈显著的正相关;土壤根量与土体崩塌时间呈显著的正相关.灌草草地植被盖度、凋落物量和根系重量的显著提高,有效地提高草地的持水量和有效截流量,推迟草地土壤崩解时间,增加了土体的稳定性,对防止水土流失和泥石流的形成具有重要作用.%In order to recover ecological environment and enhance soil and water conservation capacity of degraded mountainous grasslands in the hot arid valley,7 combined patterns for shrub-grass communities were established on the degraded mountains in the Jinsha River hot-arid valley at Yongsheng County of Yunnan Province from 2004 to 2007. In 2007,litter weight,the largest water-holding capacity,effective cut-off flow,grass cover and grassland soil disintegration time of grassland in this hot arid valley were analyzed. The results showed that "shrub+grass+vine" was the most favorable pattern for vegetation recovery in the area of Jinsha River hot-arid valley. Among them,the combination of "Cajanus cajan + Setaria sphacelata + Festuca arundinacea + Macroptilium atropurpureum" significantly improved the grassland vegetation coverage and litter weight. The water retention capacity was significantly correlated with weight of the litter. Soil root biomass was significantly positively correlated with the soil disintegration time

  12. 高温和低湿对干热河谷7个植被恢复树种光合参数的影响%Influence of high temperature and low humidity on photosynthetic parameters of seven tree species used for vegetation restoration in dry-hot valley

    Institute of Scientific and Technical Information of China (English)

    段爱国; 张建国; 何彩云

    2013-01-01

    采用人工气候室控制环境条件,研究了高温(30℃和40℃)对印楝(Azadirachta indicaA.Juss.)、木豆[Cajanus cajan(Linn.) Huth]、赤桉(Eucalyptus camaldulensis Dehn.)、蓝桉(E globulus Labillardière)、柠檬桉(Ecitriodora Hook.)、大叶相思(Acacia auriculiformisA.Cunn.)和麻风树(Jatropha curcas Linn.)7个干热河谷植被恢复树种幼苗叶片光合参数的影响,以及低湿条件对麻风树幼苗叶片光合参数的影响,并对高温及低湿条件下各树种叶片光合参数与叶片含水量的关系进行了探讨.结果表明:在高温条件下,供试树种叶片的净光合速率(Pn)和Fv/Fm值均随叶片含水量的提高逐渐增加,但在40℃条件下各树种的Pn以及Fv/Fm值均明显降低,表明叶片含水量高有利于各树种叶片Pn和Fv/ Fm的提高,而40℃高温则对其Pn和Fv/Fm有明显抑制作用.在非干旱条件下,高温对各树种幼苗的Pn、气孔导度(Gs)、胞间CO2浓度(Ci)和蒸腾速率(Tr)均有不同程度影响.在40℃条件下,供试树种中印楝和赤桉幼苗的Pn降幅最小;大叶相思、蓝桉和木豆幼苗的Gs明显增加;各树种幼苗的Ci均明显提高;除印楝外,其他6个树种幼苗的Tr明显加剧且叶片水分利用效率降低.在叶片含水量较高的状况下,供试7个树种中印楝和赤桉幼苗的Fv/Fm值受高温的影响较小.在空气相对湿度20%的条件下,麻风树幼苗的Pn、Gs、Ci及Tr均明显降低,且均随叶片含水量的提高而增加,表明叶片含水量下降是麻风树幼苗光合作用减弱的重要影响因素.研究结果表明:干热河谷地区的高温引起的植物光合限制以非气孔因素为主导;在供试的7个树种中,印楝和赤桉对干热河谷高温干旱生境的适应或忍耐能力较强.

  13. Influência da calagem no rendimento de matéria seca de plantas de cobertura e adubação verde, em casa de vegetação

    Directory of Open Access Journals (Sweden)

    P. R. Ernani

    2001-12-01

    Full Text Available A recuperação de solos degradados é lenta e onerosa. Plantas de cobertura de solo e adubação verde têm sido amplamente utilizadas nesse processo de recuperação, pois promovem alta produção de fitomassa com baixo custo, especialmente se forem tolerantes à acidez. O presente trabalho objetivou avaliar o efeito da calagem no rendimento de matéria seca de dezesseis dessas espécies, em casa de vegetação. Oito espécies de inverno (Lollium multiflorum, Avena strigosa, Lathyrus sativus, Lupinus angustifolius, Pisum sativum, Secale cereale, Vicia sativa e Vicia villosa e oito de verão (Stizolobium niveum, Stizolobium aterrinum, Stizolobium deeringianum, Crotalaria juncea, Crotalaria retusa, Crotalaria spectabilis, Cajanus cajan e Canavalia ensiformes foram cultivadas em amostras da camada arável de dois solos ácidos catarinenses (Latossoso Bruno e Cambissolo Húmico, em Lages (SC, em 1997. Os tratamentos consistiram da aplicação de doses de calcário equivalentes a 0, 0,25, 0,50, 1,00 e 1,50 vezes a quantidade recomendada pelo método SMP para elevar o pH dos solos a 6,0. Os valores de Ca e Mg trocáveis aumentaram linearmente com a calagem e na mesma magnitude, ao redor de 3,0 mmol c kg-1 para cada incremento de 0,1 unidade de pH. O efeito da calagem no rendimento de matéria seca variou com o solo e, principalmente, com a espécie. Dez das 16 espécies não apresentaram aumento no rendimento de matéria seca com o aumento do pH, em pelo menos um dos solos, e somente cinco espécies responderam à calagem de forma semelhante nos dois solos. O pH no qual as espécies tiveram a produção máxima de matéria seca foi igual ou inferior a 5,5 em qualquer dos solos e foi normalmente menor no Cambissolo do que no Latossolo, provavelmente pelo efeito benéfico da matéria orgânica em minimizar a toxidez do Al. Essas espécies podem, portanto, ser cultivadas com sucesso como melhoradoras das propriedades físicas, químicas e biol

  14. Teores de nitrogênio no solo e nas frações da matéria orgânica sob diferentes sistemas de produção de milho

    Directory of Open Access Journals (Sweden)

    W. J. O. Souza

    2000-12-01

    Full Text Available Com o objetivo de avaliar o efeito de sistemas de produção sobre a dinâmica do N no solo e nas frações da matéria orgânica, realizou-se, por seis anos, em Jaboticabal (SP, um experimento constituído de: semeadura convencional de milho com pousio no inverno (C-Mi-P, plantio direto de milho e pousio no inverno (D-Mi-P, plantio convencional de milho em rotação com soja e pousio no inverno (C-Mi-P-So, plantio direto de milho em rotação com soja e pousio no inverno (D-Mi-P-So e plantio direto de milho com uso de Mucuna aterrina (mucuna-preta, Cajanus cajan (feijão guandu e Crotalaria juncea no inverno (D-Mi-Mu, D-Mi-Gu e D-Mi-Cr, em delineamento de blocos ao acaso e parcelas subdivididas. Após 60 dias da emergência das plântulas, coletaram-se amostras de solo (0-0,05, 0,05-0,10 e 0,10-0,20 m e planta. Nas amostras de terra, avaliaram-se os teores de N total, N-NH4+, N-NO3-, N biomassa microbiana (N-B, N potencialmente mineralizável (NPM, N total nas frações: matérias húmicas (MH, solúvel em água (FSA, ácido fúlvico (AF, ácido húmico (AH e humina (HN com dados expressos em base de TFSE. Nas folhas, determinaram-se os teores de N total, e nas plantas de adubo verde (semeadas após a colheita do milho, mediram-se a produção de matéria seca (MS. Observaram-se valores maiores de NPM na camada de 0-0,05 m e nos tratamentos com plantio direto, envolvendo ou não rotação de culturas ou adubação verde, com a FSA se comportando de modo semelhante. O sistema de cultivo convencional, envolvendo ou não rotação de culturas, proporcionou maiores valores de N mineral na camada de 0,05-0,10 m, em razão da presença de maiores valores de N-nítrico. O cultivo convencional acelerou o processo de mineralização do N, enquanto a maior adição de matéria orgânica pela cultura de inverno no sistema de plantio direto promoveu incremento da fração potencialmente mineralizável do N no solo.

  15. Tempo de Captura de Organismos da Mesofauna do Solo e seus Reflexos na Interpretação de Índices da Comunidade Edáfica

    Directory of Open Access Journals (Sweden)

    Luiz Rodrigues Freire

    2015-10-01

    Full Text Available RESUMO A mesofauna edáfica compreende organismos de dimensões entre 100 μm e 2 mm, sendo importante avaliar os métodos utilizados para sua caracterização. Os objetivos deste trabalho foram definir o período de tempo de permanência do material de solo no extrator Berlese-Tüllgren para a captura dos organismos da mesofauna e determinar o tempo mínimo de extração para a correta utilização dos índices de Shannon, Simpson, Pielou e Margalef, empregados na avaliação da diversidade, dominância e equabilidade das comunidades da mesofauna. O estudo foi conduzido no Departamento de Solos do Instituto de Agronomia da Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, em terreno altamente antropizado. Foram instaladas parcelas experimentais com os seguintes tratamentos: feijão-de-porco (Canavalia ensiformis, mucuna-preta (Mucuna aterrima, feijão-bravo-do-Ceará (Canavalia brasiliensis, guandu (Cajanus cajan e vegetação espontânea, em delineamento de quadrado latino. Em cada uma das unidades experimentais, foram coletadas amostras formadas por serapilheira e material de solo, nos primeiros 5 cm da camada superficial do solo realizada um dia antes do plantio das leguminosas (outubro e, posteriormente, por ocasião do corte da parte aérea das plantas (março. As amostras foram submetidas a uma bateria de extratores do tipo Berlese-Tüllgren, por um período de 15 dias, realizando-se a contagem diária dos organismos. A partir da contagem e identificação em nível de grupo (Ordem/Família dos organismos capturados, foram determinados os índices de Margalef, Simpson, Shannon e Pielou, bem como verificou-se a correlação por ordem de Kendall entre os valores obtidos dia a dia até o 8º dia de extração, usando-se o índice de Shannon como referência. Concluiu-se que é possível a redução do tempo de permanência das amostras de material de solo nos funis Berlese-Tüllgren, não sendo recomendável a ado

  16. N-P-K balance in a milk production system on a C. nlemfuensis grassland and a biomass bank of P. purpureum CT-115 clone

    Science.gov (United States)

    Crespo, G.; Rodriguez, I.; Martinez, O.

    2009-04-01

    In very intensive milk production systems in Europe and America with the use of high amounts of chemical fertilizers, the nutrient recycling models consider the losses by leaching and N volatilization, as well as the hydro physical characteristics of the soil affecting the performance of this element (10; 6). However, in more extensive milk production systems, low input agriculture forming the natural cycle occurring within each farm, is of vital importance to potentate nutrient recycling for a stable animal production. The objective is the determination of the values of N, P and K inputs and outputs in a dairy farm with a sward composed by 60% of C. nlemfuensis and 40% of P. purpureum CT-115, associated with legumes in 28% of the area and the balance of these nutrients in the system using the "Recycling" software proposed by Crespo et al (2007). The grassland covered an area of 53.4 ha, composed by C. nlemfuensis (60%), P. purpureum CT-115 (40%) and L. leucocephala and C. cajan legumes intercropped in 28% of the area. The dairy herd consisted of 114 cows, 35 replacement heifers and 24 calves. There was a milk yield of 100 000 litters and the animals consumed 825 t DM from pastures and 75.1 t DM from other supplementary feeds. Nutrients extracted by pastures, nutrients intake by animals from pastures, symbiotically N fixation by legumes and N, P and K determinations outside the system due to animal production were determined (3-11). Volatilized ammonia, nutrient input and litter accumulated in the paddocks were measured once each season of the year. In the whole system the balance indicates negative values of N, P and K. Out of the total amount of nutrients consumed, animals used only 16 kg N, 5 Kg P and 4 Kg K for milk production, LW gain and calf production, the remainder returned to the system through excretions. Hence, more than 90% of the N and K, and approximately 81% of the P consumed by the animals were recycled to the system through the excretions. These

  17. Fitomassa, teor e acúmulo de micronutrientes do milheto, feijão-deporco e guandu-anão, em cultivo solteiro e consorciado = Micronutrient phytomass, levels and accumulation in millet, jack bean and pigeon pea, as sole crops and in intercropping

    Directory of Open Access Journals (Sweden)

    Cícero Monti Teixeira

    2008-10-01

    pea (Cajanus cajan, dwarf cultivar, as sole crops and intercropping gramineous with leguminous crops, for straw production in a no-tillage system. The experiment was carried out in the experimental field at the Agriculture Department of the Federal University of Lavras (Lavras, Minas Gerais state, Brazil. The experimental was conducted using a randomized block design, with seven treatments for species nutrient levels under different conditions (sole crops orintercropping and five treatments for phytomass production and micronutrient accumulation, with four replications. For phytomass production, only the pigeon pea sole crop displayed loweryield of fresh and dry phytomass, while the others produced on average 12.361 and 2.854 t ha-1 of fresh and dry phytomass yield, respectively. There were significant differences in B, Cu and Znlevels, and in accumulation of all evaluated micronutrients. The leguminous species presented the greatest B levels, with the greatest Cu and Zn levels found in millet. In general, millet plusjack bean intercropping presented the greatest micronutrient accumulation.

  18. Atributos biológicos do solo sob influência da cobertura vegetal e do sistema de manejo Soil biological attributes influenced by cover crops and management systems

    Directory of Open Access Journals (Sweden)

    Mozaniel Batista da Silva

    2007-12-01

    Full Text Available O objetivo deste trabalho foi avaliar os efeitos de culturas de cobertura e dos sistemas plantio direto (PD e convencional (PC sobre indicadores biológicos do solo, cultivado com feijoeiro-comum, no inverno, sob irrigação. O experimento foi conduzido em Santo Antônio de Goiás, GO, em Latossolo Vermelho distrófico textura argilosa. Culturas de cobertura foram implantadas anualmente no verão, desde 2001, sendo utilizadas a braquiária, guandu, milheto, capim-mombaça, sorgo, estilosantes, braquiária consorciada com milho, e mata nativa, como tratamento referência. Em 2005, 60 dias após o corte das culturas de cobertura foi implantada a cultura do feijoeiro, cultivar BRS Valente, sob irrigação, com semeadura realizada em 16/6/2005 e colheita efetuada em 19/9/2005. Coletaram-se amostras de solo, na profundidade de 0-10 cm, em três épocas: novembro de 2004 (pré-plantio das culturas de coberturas, junho (pré-plantio do feijoeiro e julho (florescimento do feijoeiro de 2005. Avaliaram-se a respiração basal, o carbono e o nitrogênio da biomassa microbiana, a razão carbono da biomassa microbiana/carbono orgânico, a razão nitrogênio da biomassa microbiana/nitrogênio total e o quociente metabólico do solo. Esses atributos biológicos do solo são influenciados pelas culturas de cobertura, manejo do solo e épocas de amostragem.The objective of this work was to evaluate the effects of cover crops and direct and conventional tillage systems on soil biological attributes when cultivated with dry bean in winter under sprinkle irrigation. The experiment was conducted in Santo Antônio de Goiás, GO, Brazil, in a clayey Rhodic Haplustox. Cover crops were cultivated annually in the summer since 2001, using Brachiaria brizantha, Cajanus cajan, Pennisetum glaucum, Panicum maximum, sorghum, Stylosanthes guianensis, brachiaria in association with corn, and native vegetation as reference. In 2005, 60 days after cutting the cover crops, BRS

  19. SUSTENTABILIDADE DE SISTEMAS ORGÂNICOS COM PLANTAS DE COBERTURA NA CULTURA DO ARROZ, POR MEIO DE ALTERAÇÕES FÍSICAS DO SOLO ORGANIC SYSTEMS SUSTAINABILITY USING COVER CROPS IN RICE CULTIVATION THROUGH SOIL PHYSICAL ATTRIBUTES CHANGES

    Directory of Open Access Journals (Sweden)

    Roberta Paula de Jesus

    2010-05-01

    Full Text Available

    O presente trabalho teve como objetivo avaliar o efeito de diferentes plantas de cobertura de solo sobre os atributos físicos de um Latossolo Vermelho distrófico, em sistema de produção orgânico, com preparo convencional. O trabalho foi conduzido, por dois anos, na área experimental da Embrapa Arroz e Feijão, em Santo Antônio de Goiás, GO, em um sistema de sucessão de plantas de cobertura-arroz, no qual as plantas de cobertura eram semeadas no outono/inverno e a cultura subsequente, o arroz, cultivar Aimoré, semeada no verão. Foram utilizadas as seguintes plantas de cobertura: mucuna preta (Mucuna aterrima, crotalária (Crotalaria juncea, guandu-anão (Cajanus cajan, sorgo vassoura (Sorghum technicum e pousio com vegetação espontânea. O delineamento experimental foi de blocos ao acaso, com quatro repetições. Foram coletadas, no segundo ano de experimentação (2005, amostras de solo, nas parcelas e em mata próxima, nas profundidades de 0,00-0,10 m; 0,10-0,20 m; e 0,20-0,30 m, para determinação do teor de matéria orgânica (MO, atributos físicos, índice S e estado de agregação do solo. O manejo das plantas de cobertura de solo foi efetuado por ocasião do florescimento. A biomassa foi incorporada ao perfil por meio de aração. Para a porosidade total, microporosidade e densidade do solo, não foram observadas diferenças significativas entre as profundidades, para as diferentes plantas de cobertura do solo. Para o índice S, observou-se, para todos os tratamentos, na camada 0-10 m de profundidade, valores entre 0,020 e 0,035, que caracterizam solos com qualidade f

  20. 木豆高产栽培技术

    Institute of Scientific and Technical Information of China (English)

    庞雯; 杨示英; 宗绪晓

    2001-01-01

    @@  木豆[Cajanus cajan (L.)Millsp]为蝶形花科常绿灌木,产于非洲和亚洲的热带地区,又名鸽豆、树黄豆、柳豆、三叶豆、千年豆等,是豆科木豆属一年生或多年生木本植物,具有粮食、蔬菜、饲料、柴火、放养紫胶虫等功能,还可作覆盖作物、蜜源作物、防护林、薪碳林、药用植物等,用途很广[1]。从1998年起,我们就从国际半干旱热带作物研究所引进了一批木豆新品种进行试种,1998年和1999年在广西南宁、邕宁、都安、河池、凤山、隆安、环江等7个县市的试种结果表明,引进的木豆新品种基本上都适应广西各个地方的气候条件,有些品种生长得非常茂盛,每667 m2可收获干籽粒100 kg以上,鲜枝叶的生物产量当年可达3 500 kg以上,而且营养价值高,是极好的粮食、蔬菜和饲料。另外,由于木豆生长较快,无论是在肥沃的土壤上还是在瘦瘠的石头山上都能生长良好,经过砍伐后第二年能生长得更茂盛,所以木豆也是造林绿化,防止水土流失,保护生态环境的好作物。现将木豆的高产栽培技术介绍如下。 1 选地整地   木豆对各种质地及pH5.0~8.5的土壤有着广泛的适应性,能够很好地生长,但对盐碱较为敏感。因此,种植木豆时最好避开盐碱地。同时,由于木豆不耐水涝,选地时还应避开雨季容易积水的低洼、土壤透水性差的地块。种植木豆的地块一般选在旱薄地域丘陵坡地其他作物很难生长的地方,以充分发挥这种作物耐旱、耐瘠的优势。用于种植木豆的地块,播种前犁15 cm深的土层足以保证木豆生长良好。耕后起畦,畦宽2 m,播种时因品种不同设计不同的行数,早熟矮生品种每畦播3行,中、迟熟品种每畦播2行,形成大小行条带种植模式较为理想。畦中可以间作花生、大豆、玉米等作物2

  1. 木豆种子萌发生理对干旱胁迫的响应--岩溶水和外源水处理的对比%Response of Germination Physiology of Cajanuscajan Seeds to Drought Stress:Comparison between Karst Water and Allogenic Water Treatments

    Institute of Scientific and Technical Information of China (English)

    黄芬; 程阳; 曹建华

    2015-01-01

    In this paper, responses of germination physiology of pigeon pea (Cajanus cajan) seeds to drought stress in karst water environment and non-karst (allogenic) water environment were studied to explore the adaptability of pigeon pea to karst environment. The results showed that: (i) Under drought stress of 20% PEG-6000, the germination rate, vigor index, germination index and biomass of pigeon pea seeds on day 7 cultivated with karst water were all greater than that of the allogenic water treatment group, while the seed germination stress index was signiifcantly smal er than that of the al ogenic water treatment group, suggesting that karst water environment was more favorable to pigeon pea seed germination. (i ) Without drought stress, the malondialdehyde (MDA) and superoxide dismutase (SOD) activities of pigeon pea seeds cultivated with karst water were al smal er than that of the allogenic water group. However, under drought stress, the SOD activity was signiifcantly higher than that of allogenic water group, suggesting pigeon pea SOD in karst water was able to more rapidly respond to external drought stress, and increase its own activity to reduce the damage to the plants. And (iii) with and without drought stress, the soluble protein level of the karst water group was higher than that of the al ogenic water group, while the free amino acid level was lower than that of the al ogenic water group. This difference was more signiifcant with the presence of drought stress, suggesting that the karst water environment was more favorable to the accumulation of soluble proteins and thus produced larger biomass. Hence, pigeon pea is a tree species that is adapted to high-calcium, alkaline environments in karst areas, and is of great signiifcance for the revegetation and rocky desertiifcation control in mountainous karst areas.%本文通过对比岩溶水环境与非岩溶水环境(外源水)中木豆种子的萌发生理对干旱胁迫的响应,来探究木豆对

  2. REGULADOR DE CRESCIMENTO E Trichoderma harzianum APLICADOS EM SEMENTES DE FEIJOEIRO CULTIVADO EM SUCESSÃO A CULTURAS DE COBERTURA GROWTH REGULATOR AND Trichoderma harzianum APPLIED TO COMMON BEAN SEEDS CULTIVATED IN SUCCESSION TO COVER CROPS

    Directory of Open Access Journals (Sweden)

    Marcos Antônio Machado Mesquita

    2010-10-01

    ônios vegetais; controle biológico; Phaseolus vulgaris L.; sistema plantio direto; Cerrado.

    The objective of this study was to evaluate the effect of growth regulator and Trichoderma harzianum in common bean, in succession to different cover crops. The experimental design consisted of split-plots, in randomized complete blocks, with four replications. For plots, the leguminous Cajanus cajan, Stylosanthes guianensis, and Crotalaria spectabilis, and the grasses Pennisetum glaucum, Panicum maximum, Brachiaria brizantha, B. brizantha associated with Zea mays, and Sorghum bicolor were used as cover crops. The subplot treatments were: control; 500 mL of growth regulator (GR; 1,250 mL of biological fungicide (BF; and 500 mL of GR plus 1,250 mL of BF, in 100 kg of seeds. Eighty-four days after the cover crops cutting, the common bean cultivar BRS Valente was sown. The root rot severity caused by

  3. EVALUACIÓN In Vitro DEL EFECTO DE FOSFOLÍPIDOS SOBRE LA GERMINACIÓN DE SEMILLAS DE TOMATE (Lycopersicon esculentum Mill)

    OpenAIRE

    María del C. Travieso; Oriela Pino; Yaíma Sánchez; Miriam Rojas; Belkis Peteira

    2015-01-01

    Se evalúo el efecto de una mezcla de fosfolípidos de origen natural, aplicados como sustrato en papel de filtro, sobre la germinación in vitro de semillas de tres cultivares de tomate (Amalia, Campbell 28 y Vita). En todos los casos se evidenció un efecto inductor sobre el crecimiento radicular de las plántulas de las semillas recién germinadas, dados por longitudes de radículas significativamente superiores en los grupos tratados con bajas concentraciones con respecto a los controles. Aunque...

  4. Viabilidad en tetrazolio de semillas de caléndula y eneldo

    OpenAIRE

    Victoria T. Jorge A.

    2006-01-01

    COMPENDIO Se realizaron pruebas con diferentes concentraciones de 2, 3, 5 Cloruro de Tetrazolio y varios tiempos de exposición de las semillas a diferentes temperaturas. Igualmente, con el fin de generar patrones topológicos se evaluó la relación de la viabilidad con plántulas normales, anormales y semillas no germinadas y con la siembra de estructuras con lesiones intencionales para determinar su viabilidad. La prueba de viabilidad demostró que la solución de tetrazolio al 1% por ...

  5. Comunicación corta. RADIOESTIMULACIÓN DE LA GERMINACIÓN EN Stevia rebaudiana CULTIVAR KH-IAN VC-142 (EIRETÉ), MEDIANTE EL EMPLEO DE RAYOS GAMMA 60CO

    OpenAIRE

    María C. González; Héctor D. Nakayama

    2015-01-01

    El presente trabajo tuvo como objetivo evaluar el efecto de bajas dosis de rayos gamma 60 Co en la germinación de las semillas de Stevia rebaudiana . Para ello se irradiaron semillas del cultivar KH-IAN VC-142 (Eireté), con dosis de 10 a 90 Gray (Gy). Se determinó que todas las dosis empleadas incrementaron el número de semillas germinadas pero la dosis de 10 Gy fue la que provocó la mayor estimulación en la germinación de las semillas.

  6. Reproducción sexual e influencia de sustratos en el desarrollo de Malpighia glabra L. (Malpighiaceae)

    OpenAIRE

    Apolinar García-Hoyos; Jesús Sánchez-Robles; Luis Arturo García-Hernández; Fernando De León-González

    2011-01-01

    Malpighia glabra, también conocida como acerola o semeruco dentro de su amplia distribución produce frutos que cuando alcanzan el estado de madurez son muy ricos en vitamina C. El presente estudio tuvo como objetivo el reproducir esta especie mediante la siembra de semillas en charolas germinadoras bajo condiciones de vivero y utilizando tres tipos de sustratos comerciales peat moss , andosol y vertisol. Se registró el porcentaje de germinación, se cuantificó el número de semillas germinadas...

  7. EFECTO DEL ESTRÉS HÍDRICO Y SALINO SOBRE LA GERMINACIÓN DE Atriplex nummularia (CHENOPODIACEAE) Effect of Water and Saline Stress on Germination of Atriplex nummularia (Chenopodiaceae)

    OpenAIRE

    MÓNICA B. RUIZ; CARLOS A. PARERA

    2013-01-01

    Los suelos salinos, característicos de zonas áridas, pueden afectar la germinación de las especies por presentar bajos potenciales hídricos o por toxicidad iónica. En este trabajo se determinó el efecto del estrés hídrico y salino sobre la germinación de Atriplex nummularia, una alternativa forrajera para zonas áridas. Las semillas fueron escarificadas para minimizar el efecto inhibidor de las brácteas y germinadas a 23 ºC sobre papel de germinación en soluciones con diferente potencial hídri...

  8. Micropropagação do jaborandi

    OpenAIRE

    Sabá Renata Tuma; Lameira Osmar Alves; Luz José Magno Queiroz; Gomes Ana Paula do Rosário; Innecco Renato

    2002-01-01

    O jaborandi (Pilocarpus microphyllus Stapf) é uma árvore da família Rutaceae destacando-se por intensivo uso na indústria farmacêutica através de um dos seus princípios ativos, pilocarpina, utilizado no controle do glaucoma. O objetivo do presente trabalho foi desenvolver um protocolo de micropropagação de jaborandi. Os ápices e segmentos caulinares utilizados na micropropagação foram retiradas das plântulas germinadas in vitro cultivadas em meio MS com diferentes concentrações e combinações ...

  9. Micropropagação do jaborandi Micropropagation of the jaborandi

    OpenAIRE

    Renata Tuma Sabá; Osmar Alves Lameira; José Magno Queiroz Luz; Ana Paula do Rosário Gomes; Renato Innecco

    2002-01-01

    O jaborandi (Pilocarpus microphyllus Stapf) é uma árvore da família Rutaceae destacando-se por intensivo uso na indústria farmacêutica através de um dos seus princípios ativos, pilocarpina, utilizado no controle do glaucoma. O objetivo do presente trabalho foi desenvolver um protocolo de micropropagação de jaborandi. Os ápices e segmentos caulinares utilizados na micropropagação foram retiradas das plântulas germinadas in vitro cultivadas em meio MS com diferentes concentrações e combinações ...

  10. Desenvolvimento e anatomia de plantas de carrapicho-beiço-de-boi (Desmodium canum (Gmel) Schinz et TheII) pré tratadas com trifluralin e fluchloralin Development and anatomy of Desmodium canum (Gmel) Schinz et Thell, plants pre-treated with trifluralin and fluchloralin

    OpenAIRE

    S.R. Machado; G.M. Corso

    1984-01-01

    Sementes de carrapicho-beiço-de-boi (Desmodium canum) foram germinadas em placas de Petri, sobre papel de filtro, com diferentes concentrações de trifluralin e fluchloralin (0, 0,5, 2,5, 12,5 e 62,5 ppm). Após 28 dias nesse meio, as plântulas foram transferidas para vasos contendo solo sem herbicida, onde permaneceram até os 101 dias de desenvolvimento. O ensaio foi conduzido em condições de labora tório, com temperatura variando entre 25-28°C. Plantas com 101 dias de desenvolvimento, prétrat...

  11. Comunicación corta. RADIOESTIMULACIÓN DE LA GERMINACIÓN EN Stevia rebaudiana CULTIVAR KH-IAN VC-142 (EIRETÉ, MEDIANTE EL EMPLEO DE RAYOS GAMMA 60CO

    Directory of Open Access Journals (Sweden)

    María C. González

    2015-01-01

    Full Text Available El presente trabajo tuvo como objetivo evaluar el efecto de bajas dosis de rayos gamma 60 Co en la germinación de las semillas de Stevia rebaudiana . Para ello se irradiaron semillas del cultivar KH-IAN VC-142 (Eireté, con dosis de 10 a 90 Gray (Gy. Se determinó que todas las dosis empleadas incrementaron el número de semillas germinadas pero la dosis de 10 Gy fue la que provocó la mayor estimulación en la germinación de las semillas.

  12. Composição da matriz de encapsulamento na formação e conversão de sementes sintéticas de pimenta-longa Composition of the encapsulation matrix on the formation and conversion of synthetic seeds of long pepper

    OpenAIRE

    Jonny ES Pereira; Rodrigo da S Guedes; Frederico Henrique da S Costa; Gottfried Cristian B Schmitz

    2008-01-01

    A pimenta-longa (Piper hispidinervium C. DC.) é uma planta nativa caracterizada pela produção de safrol, um metabólito secundário usado na produção de perfumes, cosméticos e inseticidas. Neste trabalho avaliou-se o uso potencial da tecnologia de sementes sintéticas como alternativa à propagação in vitro de pimenta-longa. Foi estudada a influência da constituição da cápsula sobre a formação e conversão in vitrode unidades encapsuláveis. Sementes pré-germinadas de pimenta-longa com 21 dias de c...

  13. Propagação in vitro de Sacha inchi In vitro propagation of Sacha inchi

    OpenAIRE

    Stevan Ricardo Bordignon; Glaucia Maria Bovi Ambrosano; Paulo Hercilio Viegas Rodrigues

    2012-01-01

    O objetivo do presente trabalho foi avaliar in vitro a relação auxina:citocinina a fim de obter propágulos nos segmentos distintos do epicótilo e hipocótilo de sementes germinadas in vitro de Sacha inchi (Plukenetia volubilis Linneo). Os segmentos apical (A), mediano (B) e o basal (C) foram introduzidos em meio de cultivo MS, semi sólido (2,0g L-1 Phytagel), suplementado com vitaminas de MS, sacarose (30,0g L-1) e submetidos a três doses da auxina ácido indolbutírico - IBA (0; 0,1; 0,5mg L-1)...

  14. Efecto del estrés hídrico y salino sobre la germinación de atriplex nummularia (chenopodiaceae)

    OpenAIRE

    Ruiz, Mónica Beatriz; Parera, Carlos Alberto

    2013-01-01

    Los suelos salinos, característicos de zonas áridas, pueden afectar la germinación de las especies por presentar bajos potenciales hídricos o por toxicidad iónica. En este trabajo se determinó el efecto del estrés hídrico y salino sobre la germinación de Atriplex nummularia, una alternativa forrajera para zonas áridas. Las semillas fueron escarificadas para minimizar el efecto inhibidor de las brácteas y germinadas a 23 ºC sobre papel de germinación en soluciones con diferente potencial hídri...

  15. Comportamiento de glicinina, beta-conglicinina y alfa-amilasa en semillas de soja deterioradas y no deterioradas Glycinin, beta-conglycinin and alpha-amylase behaviour in artificially deteriorated and not deteriorated soybean

    Directory of Open Access Journals (Sweden)

    Adriana Rita Salinas

    2002-08-01

    Full Text Available El objetivo del trabajo fue estudiar el comportamiento de glicinina y beta-conglicinina y la actividad de alfa-amilasa en semillas deterioradas y no deterioradas de 10 cultivares de soja [Glycine max (L. Merr.]. Las semillas se sometieron a dos tratamientos: deterioradas por envejecimiento acelerado y no deterioradas. Se determinó la presencia de las proteínas de reserva a partir de semillas con 0, 3 y 8 días de germinadas por electroforesis en geles de poliacrilamida (SDS-PAGE. La actividad de la alfa-amilasa se determinó bioquímicamente en semillas con 0, 3, 8 y 12 días de germinadas. No hubo diferencias en la presencia de bandas de glicinina y beta-conglicinina en semillas no deterioradas hasta los 8 días de germinadas. Las semillas deterioradas se comportaron en forma similar a las no deterioradas. La actividad de la alfa-amilasa aumentó en semillas germinadas hasta 8 días y disminuyó a los 12 días. En semillas deterioradas la actividad enzimática disminuyó con respecto a las no deterioradas. El deterioro artificial no afectó la presencia de glicinina y beta-conglicinina pero alteró la actividad de la alfa-amilasa hasta los 12 días de germinación. Los cultivares estudiados mostraron comportamiento diferencial frente a la actividad de esta enzima.The objective of this research was to study the behaviour of the storage proteins glycinin and beta-conglycinin and the alpha-amylase activity in artificially deteriorated and not deteriorated soybean [Glycine max (L. Merr.] seeds of 10 cultivars. The seeds were submitted to two treatments: deteriorated by accelerated aging and not deteriorated seeds. The presence of glycinin and beta-conglycinin were determined in seeds with 0, 3 and 8 days of germination using polyacrylamid gel electrophoresis (SDS-PAGE. The alpha-amylase activity was biochemically determined in seeds with 0, 3, 8 and 12 days of germination. There were no differences in the presence of glycinin and beta

  16. Propagação in vitro de Ocimum basilicum L. (Lamiaceae) - DOI: 10.4025/actascibiolsci.v25i2.2034 In vitro propagation of Ocimum basilicum L. (Lamiaceae)

    OpenAIRE

    Fabiana Kommling Seixas; Marcia Wulff Schuch; Vera Lucia Bobrowski; Eugenia Jacira Bolacel Braga; Luciana Bicca Dode

    2003-01-01

    Ocimum basilicum L. (manjericão) pertencente à família das Lamiaceae é rico em óleos essenciais aromáticos e valorizado por suas propriedades farmacêuticas, aromáticas e culinárias. Neste trabalho descrevemos o procedimento para micropropagação de O. basilicum utilizando cotilédones de sementes germinadas in vitro. Estas foram utilizadas como explante inicial, colocadas em meio MS (Murashige e /skoog, 1962), com 0,2mg.L-1 de ANA em combinação com BAP nas variações de 0–5mg.L-1 e mantidas à 28...

  17. Establecimiento in vitro de Cedrela salvadorensis Standl

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    Bibiana Soto Vargas

    2010-12-01

    Full Text Available El objetivo del trabajo fue estableceruna metodología para introducir elprocedimiento in vitro como unaalternativa de propagación para futurostrabajos de conservación o mejoramientogenético de la especie.Como materialexperimental se utilizaron tanto plántulasde invernadero de ocho meses de edadpara la introducción de estaquillas comoplántulas de semillas germinadas in vitropara la obtención de segmentos de nudo.En la desinfección de las estaquillas seutilizó Benlate® (Benomil 0,5 gL-1 yAgrimicin® (estreptomicina 4,5 gL-1. Losdesinfectantes evaluados fueron NaOCl(3% i.a durante 10 minutos y CaOCl(9,23% i.a durante 25 minutos.Todos los explantes se colocaron en unmedio de cultivo Murashige y Skoog(1962 que se complementó con 2.7 gL-1de gelrite y cuatro concentraciones deBenciladenina (BA (0; 0,5; 1,5; 2,5y 3,5 mgL-1. El mejor método para ladesinfección de las estaquillas fue NaOCl(3% i.a durante 10 minutos. La mejorrespuesta de las estaquillas de plántulas deinvernadero se observó en la concentraciónde 0,5 mgL-1 de BA; por su parte, la mejorrespuesta de las plántulas germinadas invitro fue en 2,5 mL-1 de BA.

  18. Characterization of the Weed Seed Bank in Zero and Conventional Tillage in Central Chile Caracterización del Banco de Semillas de Malezas en Labranza Cero y Convencional en Chile Central

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    Rosa Peralta Caroca

    2011-03-01

    Full Text Available We studied the abundance, species composition, and depth distribution of the weed seed bank under no-tillage (NT and conventional tillage (CT at two sites, A and B. Soil samples were taken at three soil depths (0-2, 2-5, and 5-15 cm. Germinated, dormant, and total seeds were counted. The total number of seeds was higher (p ≤ 0.05 under CT treatment at the two sites (CTA = 5175 seeds m-2, NTA = 3250 seeds m-2, CTB = 33770 m-2, and NTB = 22437 seeds m-2. The number of viable, dormant, and germinated seeds was also higher (p ≤ 0.05 in CT at the two sites. The percentage of viable seeds was low with 37% (CTA, 34% (CTB, 21% (NTA, and 8% (NTB. Viable seeds of Chenopodium album L. (CHEAL dominated in the two trials with 67% (CTA, 20% (NTA, 96% (CTB, and 77% (NTB. In a principal component analysis, PC1 separated viable seeds of weed species according to tillage and PC2 separated weed species according to sites. Poa annua L. was the most important species associated with NT followed by Cichorium intybus L., and Sonchus while Euphorbia helioscopia L. and Echinochloa crus-galli (L. P. Beauv. were associated with CT.Se estudió la abundancia, composición de especies y distribución en profundidad del banco de semilla de maleza en labranza convencional (CT y cero labranza (NT en dos sitios, A y B. Se tomaron muestras a tres profundidades de suelo (0-2, 2-5 y 5-15 cm. Se contaron semillas germinadas, dormantes y totales. La cantidad total de semillas fue significativamente mayor (p ≤ 0,05 para ambos sitios (CTA = 5175 semillas m-2, NTA = 3250 semillas m-2, CTB = 33770 semillas m-2 y NTB = 22437 semillas m-2. La cantidad de semillas viables, dormantes y germinadas fue significativamente mayor (p ≤ 0.05 para ambos ensayos en CT que en NT. La mayor proporción de las semillas viables correspondió a germinadas. Se observó un bajo porcentaje de semillas viables 37% (CTA, 34% (CTB, 21% (NTA and 8% (NTB. Las semillas viables de Chenopodium album L. (CHEAL

  19. Produção de forragem verde hidropônica de milho, utilizando substratos orgânicos e água residuária de bovinos

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    Marco Antonio Píccolo

    2013-08-01

    Full Text Available O objetivo deste trabalho foi avaliar a produtividade da forragem verde hidropônica de milho em diferentes substratos orgânicos e em água residuária de bovinos com diferentes concentrações de nitrogênio. O delineamento estatístico foi o de blocos casualizados, em esquema fatorial (4 x 5 + 4, com três repetições. As soluções nutritivas utilizadas foram: testemunha (solução nutritiva padrão para hidroponia de milho e água residuária de bovinos, com 0, 25, 50, 75 e 100% de nitrogênio, com volume de aplicação de 4 L m-2. Os substratos utilizados foram bagaço de cana-de-açúcar, casca de café, capim elefante cv napier e sem substrato. A densidade de semeio foi de 2,5 kg m-2 de sementes de milho pré-germinadas. A colheita foi realizada 15 dias após a instalação do experimento. Avaliou-se a produção de matéria seca da parte aérea, matéria seca da base (composta por raízes + sementes não germinadas + substrato orgânico e matéria seca da planta toda. A água residuária de bovinos pode ser utilizada em substituição à solução nutritiva padrão, não havendo necessidade de seu enriquecimento com nitrogênio. Houve efeito significativo dos substratos utilizados na produção de matéria seca da forragem, com destaque para os substratos capim napier e bagaço de cana, que promoveram incrementos na produção de matéria seca da parte aérea.

  20. Propagação in vitro de Sacha inchi In vitro propagation of Sacha inchi

    Directory of Open Access Journals (Sweden)

    Stevan Ricardo Bordignon

    2012-07-01

    Full Text Available O objetivo do presente trabalho foi avaliar in vitro a relação auxina:citocinina a fim de obter propágulos nos segmentos distintos do epicótilo e hipocótilo de sementes germinadas in vitro de Sacha inchi (Plukenetia volubilis Linneo. Os segmentos apical (A, mediano (B e o basal (C foram introduzidos em meio de cultivo MS, semi sólido (2,0g L-1 Phytagel, suplementado com vitaminas de MS, sacarose (30,0g L-1 e submetidos a três doses da auxina ácido indolbutírico - IBA (0; 0,1; 0,5mg L-1, associadas a quatro doses da citocinina benzilaminopurina - BAP (0; 0,1; 0,5; 1,0mg L-1, totalizando 36 tratamentos. Após nove semanas de cultivo in vitro, o segmento apical (A apresentou formação de brotações por organogênese direta nas concentrações de 0,5 e 1,0 de BAP, associado a 0,0 e 0,1 de IBA. O emprego do cultivo in vitro é viável na produção de mudas, utilizando como explante a região apical de sementes germinadas in vitro.The aim of this study was to perform an in vitro evaluation of the auxin:cytokinine ratio in different segments of the epicotyl and hypocotyl of Sacha inchi (Plukenetia volubilis Linneo seeds germinated in vitro. The segments apical (A, median (B and basal (C were introduced into semi-solid MS culture medium (2.0g L-1 Phytagel, supplemented with MS vitamins, sucrose (30.0g L-1 and submitted to three doses of auxin indolebutyric acid - IBA (0; 0.1; 0.5mg L-1, associated with four doses of the cytokinine benzylaminopurine - BAP (0; 0.1; 0.5; 1.0mg L-1, totaling 36 treatments. After nine weeks of in vitro cultivation, the apical segment (A presented shoot formation by direct organogenesis at the concentrations of 0.5 and 1.0 of BAP associated with 0.0 and 0.1 of IBA. It is feasible to use in vitro cultivation with the apical region of seeds germinated in vitro used as explants.