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Sample records for cadena mediante elisa

  1. INMUNODIAGNOSTICO DE FASCIOLOSIS BOVINA MEDIANTE ELISA Y WESTERN BLOT

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    Texia Gorman

    1998-01-01

    Full Text Available Se evaluó y caracterizó la respuesta inmune humoral de bovinos naturalmente infectados con Fasciola hepatica frente a un extracto total de antígenos de excreción-secreción (E-S y a una fracción antigénica semipurificada cromatográficamente (Immunodiagnosis of bovine fasciolosis was evaluated in terms of its sensitivity and specificity by ELISA and Western blot using a crude excretory-secretory preparation (C E-S and an E-S partially purified chromatographic fraction of Fasciola hepatica (<30 kDa as antigens. Serum samples from 52 bovines with natural fasciolosis, 18 without the infection and 48 bovines with hydatid cysts, all checked by post mortem examination, were tested as well. The sensitivity and specificity obtained by ELISA using the C E-S antigens were 53% and 100%, respectively. Likewise, when using the partially purified fraction, the sensitivity of ELISA test increased to 90% with a 100% specificity. The C E-S antigens reacted specifically with sera from infected bovines exhibiting bands of 14, 22, 27-39 and 37-38 kDa in Western blots. When the partially purified fraction was tested, the 28-30 kDa band was consistently detected by sera from all infected bovines and was absent in sera from uninfected as well as in sera from those with hydatid infection. These results indicate that F. hepatica presents different antigens, among which the 28-30 kDa fraction represents polypeptides with diagnostic potential that can be further purified and applied in the immunodiagnosis of bovine fasciolosis corroborating previous results recorded in other animal species

  2. Uso della chemiluminescenza per la diagnosi della brucellosi bovina e ovina mediante ELISA indiretta e competitiva

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    Manuela Tittarelli

    2008-06-01

    Full Text Available I metodi ufficiali previsti dal Piano nazionale di eradicazione della brucellosi bovina ed ovi-caprina sono la sieroagglutinazione rapida (SAR con l’antigene acidificato al Rosa Bengala e la fissazione del complemento (FDC. Nella attuale fase del piano di eradicazione non è infrequente imbattersi in risultati di difficile interpretazione ottenuti con i test ufficiali, pertanto è necessario poter disporre di test aggiuntivi che presentino livelli di specificità e di sensibilità più elevati. A questo scopo sono stati validati due metodi ELISA, indiretto (i-ELISA CL e competitivo (c-ELISA CL, mediante l’utilizzo di un substrato chemiluminescente per la determinazione di anticorpi anti-Brucella in siero bovino ed ovino. I metodi si basano sulla rivelazione degli anticorpi anti-Brucella, contenuti nel siero, mediante la catalisi di un substrato enzimatico chemiluminescente (sistema luminolo/H2O2/ enhancer, da parte della perossidasi coniugata ad anticorpi secondari anti-IgG, nella i-ELISA CL, o al monoclonale anti-LPS, nella c-ELISA CL. Sulla base dei risultati ottenuti, per l'i-ELISA CL è stato stabilito un valore di cut-off, espresso come percentuale di positività (PP, del 60% per i sieri bovini e del 37,5% per quelli ovini; con questo valore di cut-off si ottiene una sensibilità ed una specificità del test del 100% per i sieri bovini, e una sensibilità del 100% e una specificità del 99,8% per quelli ovini. Per la c-ELISA CL è stato scelto un cut-off, espresso come percentuale di inibizione (PI, del 30% per i sieri bovini e del 40% per quelli ovini, che assicura valori di sensibilità e specificità del 100% in entrambi i casi.

  3. Determinación de los impactos ambientales potenciales en la cadena de suministro de crisantemo (Dendranthema grandiflora) mediante un enfoque de análisis de ciclo de vida

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    Ricardo Hernández, Ricardo Esteban

    2013-01-01

    Resumen: Colombia es uno de los principales países exportadores de flores con una importante variedad de especies, entre las cuales el crisantemo (Dendranthema grandiflora) ocupa un destacado lugar de acuerdo a su volumen de exportación y calidad en los mercados internacionales. Dada la relevancia de este producto, en esta investigación se identificaron los principales impactos ambientales generados en su cadena de suministro mediante análisis de ciclo de vida –ACV, bajo los parámetros de ISO...

  4. Un estudio mediante cadenas de Markov de la dinámica de los movimientos migratorios interterritoriales en España (1990-2003) desde un planteamiento de estimación dinámico

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    Hierro Franco, María; Guijarro Garvi, Marta

    2006-01-01

    RESUMEN: El análisis de los movimientos migratorios mediante cadenas de Markov más frecuente considera la existencia de homogeneidad temporal, estimándose sus probabilidades de transición con técnicas de naturaleza estática. El fenómeno migratorio es, sin embargo, propiamente dinámico con influencia de variables muy diversas y de difícil previsión. Por ello, este trabajo propone el método dinámico de medias móviles, que proporciona mejores resultados que el habitual métod...

  5. Determinación de Glifosato mediante inmunoensayo enzimático (ELISA en el Paisaje Protegido Laguna de Rocha y su entorno, Uruguay.

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    Daniela Nardo

    2015-12-01

    Full Text Available En el entorno de la Laguna de Rocha se ha visto incrementada la superficie dedicada a las actividades agrícolas con un mayor uso de plaguicidas, entre ellos el herbicida glifosato, usado en cultivos de soja principalmente. Mediante la utilización de técnicas de inmunoensayo enzimático (ELISA, se investigó la presencia de glifosato en la Laguna y algunos de sus afluentes en dos momentos específicos de tiempo. Se detectó glifosato en 27 de las 28 muestras estudiadas. Muestras positivas por ELISA fueron confirmadas por cromatografía iónica. El método ELISA demostró ser una herramienta de screening adecuada para determinar la presencia de glifosato en agua.

  6. Cambios en el patrón de desarrollo floral de Neoregelia 'Flandria' (Bromeliaceae analizados mediante Cadenas de Markov Floral Developmental Pattern Changes on Neoregelia‘Flandria’(BROMELIACEAE Analysed by Markov Chain methodologyADRIANA

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    Chaparo de Barrera Ángela

    2003-06-01

    Full Text Available Neoregelia 'Flandria'y N. 'Van Durme'son cultivares ornamentales de bromelias. Sussemillas no son viables y la propagación in vitrocausa alteraciones en el variegado delfollaje, por tanto la brotación vegetativa se constituye en la única opción de propa-gación estable. Este artículo hace referencia a los patrones de desarrollo floral (p de72 bromelias y su relación con la brotación vegetativa por efecto de 3 tratamientos:ANA 190 ppm (T1, Ethrel: ANA + ETHREL (T2 y ETHREL, (T3, y divididos poredad: E1 y E2. El resultado de la aplicación de estos tratamientos fue que se obtu-vieron plantas más elongadas y 6 nuevos patrones. Mediante la metodología de cade-nas de Markov se estimaron la evolución de cada patrón hacia los demás a nivel deprobabilidad (si/no y el porcentaje de esta para cada caso.Neoregelia 'Flandria'and N. 'Van Durme'are ornamental cultivars of Bromelia.Propagation by seeds is not viable and prunes constitutes the only way to propagateavoiding alterations. In this article the developmental floral pattern of 72 Bromeliastreated with ANA 190 ppm (T1, Ethrel: ANA + ETHREL,(T2 y ETHREL, (T3 anddivided into two age groups: E1 y E2 are shown. The treatments studied generated moreelongated plants and six new patterns. Using the Markov chain methodology theprobability to evolve to any pattern and the percentage of each were studied.

  7. Valutazione della produzione di gamma interferone in bovini vaccinati con Brucella abortus ceppo RB51 mediante un test ELISA

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    Manuela Tittarelli

    2009-06-01

    Full Text Available In questo lavoro sono presentati i risultati di un test ELISA messo a punto per rilevare la produzione di gamma interferone (g-interferone in bovini vaccinati con Brucella abortus ceppo RB51 (RB51. Come stimolo antigenico per il sangue intero è stata utilizzata una frazione proteica purificata derivante da RB51 (brucellina RB51. La prova è stata valutata nell’arco di 300 giorni in 10 manze vaccinate in età prepubere con 10×109 Unità Formanti Colonia di RB51 e in cinque manze di controllo, provenienti da allevamenti ufficialmente indenni da brucellosi bovina. I capi vaccinati hanno cominciato a fornire risultati positivi a partire dal 17° giorno post vaccinazione (p.v. fino al giorno 239 p.v. Tutti i capi vaccinati hanno fornito almeno una volta un risultato positivo (indice di stimolazione, IS, superiore a 2,5. Tuttavia, se si esclude il prelievo al giorno 20 p.v. (90% di animali vaccinati risultati positivi, la sensibilità del test oscilla tra il 20% e il 70%, con una media del 40%. IS superiore a 2,5 è stato rilevato anche in tre animali di controllo. Sulla scorta dei risultati ottenuti, si ritiene che il test del g-interferone non fornisce garanzie sufficienti per consigliarne l’impiego ai fini di riconoscere i bovini vaccinati con RB51, sia come prova individuale, sia come prova d’allevamento.

  8. Los cuatro arquetipos de cadenas de suministro

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    Salvatore Cannella

    2010-01-01

    Full Text Available A lo largo del siglo XX las cadenas de suministro han evolucionado para enfrentarse a los desafíos del mercado globalizado y librarse de ineficiencias congénitas como el efecto látigo. En este artículo se presentan cuatros arquetipos de cadenas de suministro, desde la clásica estructura tradicional hasta el innovador paradigma de la configuración sincronizada. Los cuatros arquetipos se presentan según una analogía con los depósitos de agua. Como conclusión, se pone de manifiesto cómo a pesar de los sobresalientes beneficios de las cadenas de nueva generación existen algunas características claves que pueden obstaculizar su implementación. El marco presentado permite a las empresas comprender las problemáticas debidas a las interacciones con sus socios/competidores y a descubrir oportunidades de mejora que se pueden alcanzar mediante mecanismos innovadores de cooperación soportados por las TIC.

  9. Algoritmo paralelo para el cálculo de matrices de probabilidades de transición: aplicación a la modelación de yacimientos lateríticos mediante cadenas de markov

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    Trinchet., Dannier; Guirola, Asnay

    2011-01-01

    En el presente trabajo se propone un algoritmo paralelo para la obtención de matrices de probabilidades de transición. El algoritmo propuesto es aplicado a la modelación de yacimientos lateríticos a partir de un modelo matemático basado en cadenas de Markov. El modelo genera un hipercubo de probabilidades condicionales, cuya cantidad de dimensiones queda fijada por la cantidad de variables que intervienen en el modelado. Se realiza un análisis teórico del algoritmo y se implementa en dos vari...

  10. Contra la cadena perpetua

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    Arroyo Zapatero, Luis

    2016-01-01

    La incorporación al Código penal de la cadena perpetua en la reforma de 2015 ha recibido un rechazo casi unánime por parte de la ciencia penal española, que la censura por su frontal oposición a nuestra Carta Magna al vulnerar la prohibición de penas inhumanas y degradantes (art. 15), los principios de culpabilidad y proporcionalidad, el derecho a la libertad (art. 17), el mandato de determinación de las penas (art. 25.1) y el de resocialización (art. 25.2).Este libro es fruto del debate doct...

  11. Evaluación de la longitud de los telómeros en caballos, mediante el uso de la reacción en cadena de la polimerasa cuantitativa

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    L. Sanmartín-Sánchez

    2014-06-01

    Full Text Available Introducción: Los telómeros son estructuras de los cromosomas eucariotas, que juegan un importante papel en el mantenimiento de la estabilidad e integridad celular, estableciéndose como uno de los posibles biomarcadores del envejecimiento celular en el hombre y otras especies. Objetivos: Evaluar la posibilidad del uso de la técnica de la reacción en cadena de la polimerasa cuantitativa (qPCR para calcular la longitud relativa de los telómeros en caballos y asociarla con factores como la edad, la raza y el y el sexo. Material y métodos: Se amplificó una secuencia repetitiva correspondiente a los telómeros, junto con otra de un gen de copia única (Interleukina-2, en muestras de sangre de 175 caballos de tres razas, de distintos grupos de edad y de ambos sexos. Se calculó la longitud relativa de los telómeros de cada individuo a través dos variables de respuesta: la diferencia entre el número de ciclos de cuantificación entre telómeros e Interleukina-2 (Dif Cq y la ratio entre ambos valores (T/S ratio. Resultados: La variable Dif Cq presenta una asociación más fuerte con la edad que la variable T/S ratio. Se observó una correlación negativa entre la edad y la longitud de los telómeros (R= -0,31, p<0,05. Conclusión: La técnica de qPCR es aplicable para determinar la longitud relativa de los telómeros en caballos. Los individuos mayores de 20 años presentan telómeros acortados, aunque no se encontró un efecto significativo ni de la raza, ni del sexo.

  12. Detección de antígenos de la larva de Taenia solium mediante la Técnica de Elisa

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    Carmenza Murillo

    2010-07-01

    Full Text Available Se describe la optimización de las variables en un ELISA sandwich asimétrico para la detección de antígenos circulantes de larva de Taenia solium empleado como estándar líquido intraquístico obtenido del parásito y como suero itunune anticisticerco absorbido con líquido cefalorraquídeo (LCR normal lo que permite detectar 50 ng/ mi de antígeno. Se utilizaron muestras de LCR liofilizadas; bajo estas condiciones de almacenamiento la especificidad obtenida fue del 63% al analizar 24 LCR negativos para neurocisticercosis por tromografía computarizada cerebral, y 15 LCR de pacientes que mostraban calcificaciones. El coeficiente de variación interensayo está dentro de los límites aceptados para el ELISA.

  13. Detección de antígenos de la larva de taenia solium mediante la técnica de elisa

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    Carmenza Murillo; Claudia Díaz; Pablo Lorenzana; Santiago Nicholls; Augusto Corredor

    2010-01-01

    Se describe la optimización de las variables en un ELISA sandwich asimétrico para la detección de antígenos circulantes de larva de Taenia solium empleado como estándar líquido intraquístico obtenido del parásito y como suero itunune anticisticerco absorbido con líquido cefalorraquídeo (LCR) normal lo que permite detectar 50 ng/ mi de antígeno. Se utilizaron muestras de LCR liofilizadas; bajo estas condiciones de almacenamiento la especificidad obtenida fue del 63% al analizar 24 LCR ne...

  14. Gobernabilidad y ascenso en la cadena de valor: discusión conceptual

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    Seyka Verónica Sandoval Cabrera

    2012-01-01

    La transformación de la base correspondiente al proceso de acumulación expresada mediante el paradigma informático-neoliberal, conduce a la reformulación en cuanto a las estrategias de desarrollo de países y empresas. En el marco de las cadenas de valor global, la estrategia sobre el desarrollo, considera el incremento de la participación en el flujo de valor, respecto a las diferentes rentas producidas en la cadena como centro. La revalorización, el ascenso o upgrading depende, en primera in...

  15. Riesgo de exposición a Fasciola hepática en ganado vacuno en extensivo de Uruguay y Portugal determinado mediante ELISA y un antígeno recombinante

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    J Sanchís

    2015-01-01

    Full Text Available Para evaluar el riesgo de exposición a Fasciola hepatica del ganado vacuno en extensivo se tomaron muestras de sangre de dos zonas climáticas diferentes, Uruguay (clima subtropical húmedo, 1.192 muestras y Portugal (clima mediterráneo, 473 muestras, que se analizaron con un ELISA y un antígeno recombinante de superficie de F. hepatica (FhrAPS. Los datos obtenidos se analizaron de acuerdo con la raza, edad sexo y aptitud. La seroprevalencia global en Uruguay resultó del 56% (Intervalo de Confianza, IC 95%: 53-59 mediante FhrAPS-ELISA, con porcentajes significativamente más altos en vacas lecheras Holstein de 2-4 años. En Portugal, el porcentaje de sensibilización fue del 47% (IC 95%: 43-52 con los valores más elevados en bovinos resultado de cruces de distintas razas, destinados a la obtención de carne y con edad también comprendida entre 2 y 4 años. Se concluyó que el riesgo de exposición a F. hepatica del ganado vacuno en extensivo es mayor en las zonas con clima subtropical húmedo que en las de clima mediterráneo. Los terneros presentan un alto riesgo de exposición al trematodo hepático con independencia de las condiciones climáticas. Los resultados ponen de manifiesto la necesidad de adoptar medidas frente a las formas del parásito que se encuentran en el medio y combinarlas con programas de desparasitación eficaces.

  16. Selección de Genotipos Chilenos de Trigo Portadores del Alelo Glutenina HMW Glu-D1 x5 Mediante Reacción en Cadena de la ADN Polimerasa Selection of Chilean Wheat Genotypes Carriers of the HMW Glutenin Allele Glu-D1 x5 through Polymerase Chain Reaction

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    Javier Zúñiga

    2004-07-01

    Full Text Available La calidad panadera es un importante elemento para el desarrollo de nuevas variedades de trigo (Triticum aestivum L. y se asocia principalmente con un conjunto de proteínas conocidas como gluteninas de elevado peso molecular (HMW, aún cuando existen otras proteínas relacionadas con la calidad panadera. Los procesos actuales de selección de genotipos con calidad panadera se basan en el contenido de gluten de la masa o la identificación de alelos codificadores de gluteninas HMW mediante electroforesis. Se reporta un método basado en la identificación, mediante la reacción en cadena de la ADN polimerasa (PCR, del alelo codificador de la glutenina HMWx5, la cual se asocia con calidad panadera. Mediante el uso de partidores apropiados se amplificó de forma específica un fragmento del promotor de este gen y parte de su extremo 5’. En aquellos genotipos portadores del gen HMWx5 se amplificó un fragmento de aproximadamente 450 pares de bases (pb el cual es consistente con el tamaño esperado a partir de su secuencia depositada en la base de datos Genbank. Su alelo HMWx2 no amplificó producto PCR alguno. El sistema fue validado en un grupo de genotipos de trigos de diverso origen y permitió la rápida e inequívoca identificación de genotipos portadores del alelo HMWx5. El desarrollo del sistema y otros análogos permitirá el desarrollo de sistemas de mejoramiento genético de trigo más efectivos y focalizados.Baking quality is a key factor in the development of improved wheat (Triticum aestivum L. cultivars and it is associated with a specific protein fraction known as High Molecular Weight (HMW glutenins, although there are other proteins involved in baking quality. Current selection of wheat germplasm with improved baking quality is based on gluten and dough analyses, or the identification of HMW glutenin alleles using electrophoretic procedures. This report presents the deployment of an allele-specific Polymerase Chain Reaction (PCR

  17. Evaluación diagnóstica de fracciones cromatográficas de Fasciola hepatica mediante Western Blot y ELISA en animales infectados Diagnostic evaluation of chromatographic fractions of Fasciola hepatica by Western Blot and ELISA in infected animals

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    F. FREDES

    1997-01-01

    Full Text Available La fasciolosis causada por Fasciola hepatica se diagnostica rutinariamente mediante el examen coprológico. Debido a que este examen no es 100% sensible y además es ineficaz en la etapa pre-patente, se realizó este trabajo con el objeto de caracterizar y seleccionar fracciones antigénicas de valor diagnóstico de extractos de excreción-secreción del parásito. Se utilizó cromatografía de exclusión por tamaño molecular (Sephacryl S-300, electroforesis en geles de poliacrilamida en ambiente reductor (SDS-PAGE y posterior western blot (WB, además de un método de "enzyme-linked immuno-sor-bent assay" (ELISA en microplaca. Para evaluar el valor diagnóstico de los antígenos se usaron sueros de las especies ovina, porcina y equina en tres estados (sanos, con otras parasitosis y naturalmente infectados con F. hepatica. Mediante el método cromatográfico se obtuvieron hasta 5 "peaks", que interpolados en una curva patrón representaron polipéptidos de pesos aproximados de 2.000, 400, 150, 29 y menores a 29 kDa. De éstos, los inmunorreactivos específicos para la enfermedad en las tres especies animales, bajo los criterios de SDS-PAGE y posterior WB, fueron los de 400, 150, 29 y The antigenic components of excretory-secretory products of adult F. hepatica, were separated by gel filtration chromatography (Sephacryl S-300 and then analized by polyacrylamide gel electrophoresis (SDS-PAGE, followed by Western Blot (WB. In order to evaluate the sensitivity, specificity and predictive value of the selected fractions an enzyme-linked immunosorbent assay (ELISA was used with sera from sheep, swine and horses infected with F. hepatica, as well as with control sera (uninfected animals. The chromatographic curve presented up to 5 peaks, representing polypeptides with a molecular weight of 2000, 400, 150, 29 and less than 29 kDa, according to the interpolation with a standard curve of commercial polypeptide molecular weights. The results obtained with

  18. Detección de proteínas prionicas en cerebros de bovino mediante inmunoensayo enzimático. (Detection of prion proteins from bovine’s brains through a ELISA.

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    Farías R., Gustavo

    2007-03-01

    Full Text Available La Encefalopatía Espongiforme Bovina (EEBpertenece a un grupo de enfermedades neurodegenerativas denominadas Encefalopatías Espongiformes Transmisibles (EETs, dentro de las cuales se destacanademás la Enfermedad de Creutzfeld-Jakob (ECJ con su nueva variante (vECJ en los humanos y el Scrapie en animales. Estas patologías se caracterizan por ser mortales, tener un largo período de incubación, signos neurológicos progresivos y por presentar lesiones vacuolares en la materia gris del sistema nervioso central (SNC. El agente etiológico de estas patologías de denomina “prión” (partícula infecciosa de naturaleza proteica, PrP y consiste en una isoforma infectiva (PrPSc, de una proteína constitutiva celular normal (PrPC que posee una gran resistencia a la proteinasa K. Pese a que en Chile no se han diagnosticado casos de EEB, es importante contar conherramientas diagnósticas de alta especificidad, rápidas y reconocidasinternacionalmente, por lo cual se realizó una inmunodetección de PrP mediante un “kit” de inmunoensayo enzimático (ELISA. Se analizaron 90 muestras de óbex bovinos mayores de 30 meses de edad, provenientesde mataderos de la Región Metropolitana. Inicialmente se obtuvieron 2 muestras sospechosas, las que luego de un nuevo ensayo se sumaron al resto de las que inicialmente fueron diagnosticadas como negativas, obteniendo finalmente un 100% de las muestras estudiadas negativas a EEB.A partir de este estudio se establecieron las pautas para la implementación de este método diagnóstico de la EEB en nuestropaís, lo cual nos permite contar con una de las herramientas diagnósticas más rápida, reconocida y utilizada a nivel internacionalpara la prevención, el control y el reconocimiento de la EEB.The bovine spongiform encephalopathy (BSE belongs toa group ofneurodegenerative disease called transmissible spongiform encephalopaties (TSEs that it includes ti Creutzfeld-Jakob`sDisease (CJD with his new variant (v

  19. Elisa Valero

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    Valero Ramos, Elisa

    2009-01-01

    MENOR O IGUAL QUÉ. Los arquitectos, habitualmente aficionados a la matemática sencilla, a los enteros más que a los quebrados, a los módulos, las series, los ejes, las simetrías y las transparencias, admiramos ese oximorón miesiano de ¿menos es más¿ que es, también, el sumum del expresionismo elemental. Y tal vez la arquitecta Elisa Valero, cuya obra autodenominada menor tengo el honor de glosar con estas letras, milita también en este orden de ideas. Educada a partes iguales entre e...

  20. Los cuatro arquetipos de cadenas de suministro

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    Salvatore Cannella; Elena Ciancimino; Framinan, Jose M.; Stephen M. Disney

    2010-01-01

    A lo largo del siglo XX las cadenas de suministro han evolucionado para enfrentarse a los desafíos del mercado globalizado y librarse de ineficiencias congénitas como el efecto látigo. En este artículo se presentan cuatros arquetipos de cadenas de suministro, desde la clásica estructura tradicional hasta el innovador paradigma de la configuración sincronizada. Los cuatros arquetipos se presentan según una analogía con los depósitos de agua. Como conclusión, se pone de manifiesto cómo a pesar ...

  1. Diseño óptimo de cadenas de sumnistros considerando criterios ambientales

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    Campanella, Sandra; Montagna, Jorge Marcelo; Corsano, Gabriela

    2014-01-01

    En el presente trabajo se propone un modelo multiobjetivo de programación mixta entera lineal para el diseño sustentable de la Cadena de Suministros (CS). Se desea determinar el diseño óptimo de la CS teniendo en cuenta las variables económicas y ambientales mediante la metodología "epsilon constraint". Para la formulación se considera una CS de 4 niveles: materia prima, plantas de producción, centros de distribución y consumidores. Para introducir la variable ambiental dentro del diseño se p...

  2. Detección de anticuerpos contra el virus de la peste porcina clásica mediante la prueba inmunoenzimática (CIV-ELISA y seroneutralización Detection of antibodies against the classical swine fever virus by enzime-linked immunosorbent assay (ELISA

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    A. ISLAS

    1997-01-01

    Full Text Available La peste porcina clásica (PPC es una enfermedad de gran importancia mundial. En Chile está sometida a un programa de control y erradi-cación. El presente ensayo tuvo como objetivo estandarizar y comparar una técnica inmunoenzimática (CIV-ELISA que detecta anticuerpos del tipo IgG dirigidos contra el virus de la PPC, con la seroneutralización (SN, técnica oficial para el diagnóstico serológico. Se utilizaron 20 cerdos híbridos Landrace x Large White de 20 kg de peso vivo, los que fueron inoculados experimentalmente con una dosis de 100.000 DICT50 del aislado virulento Quillota del virus PPC. Un grupo constituido por 4 cerdos fue vacunado 14 días antes de la inoculación. Se tomaron muestras de sangre a los 3, 6, 9, 12 y 14 días post-inoculación. Además, se obtuvieron muestras serológicas de 100 hembras reproduc-toras. Los anticuerpos anti-PPC fueron determinados por un CIV-ELISA policlonal, realizándose comparación con la prueba de SN. La prueba CIV-ELISA no detectó anticuerpos en los cerdos inoculados experimentalmente hasta los 14 días post-inoculación. Sin embargo, los cerdos vacunados previamente fueron seropositivos, situación ratificada por la SN. La sensibilidad y especificidad del CIV-ELISA fue de 90.1% y 76.4%, respectivamente. El CIV-ELISA demostró ser una prueba de fácil aplicación y los resultados son similares a los de la SNClassical swine fever (CSF is an important worldwide disease. In Chile, it is subject to a control and erradication programme. The objective of the present assay was to standardize an immunoenzimatic technique (CIV-ELISA that detects antibodies of the IgG type directed against the CSF virus and to compare it with seroneutralization. Twenty hybrid Landrace x Large White pigs with a live weight of 20 kg were used. They were experimentally inoculated with a dose of 100.000 TCID50 of the Quillota virulent isolate of the CSF virus. A four-pig group was vaccinated 14 days before inoculation

  3. Riesgo de exposición a Fasciola hepática en ganado vacuno en extensivo de Uruguay y Portugal determinado mediante ELISA y un antígeno recombinante

    OpenAIRE

    Sanchís, J.; GV Hillyer; LM Madeira de Carvalho; MI Macchi; C. Gomes; G Maldini; G. Stilwell; JM Venzal; Paz-Silva, A.; Sánchez-Andrade, R.; MS Arias

    2015-01-01

    Para evaluar el riesgo de exposición a Fasciola hepatica del ganado vacuno en extensivo se tomaron muestras de sangre de dos zonas climáticas diferentes, Uruguay (clima subtropical húmedo, 1.192 muestras) y Portugal (clima mediterráneo, 473 muestras), que se analizaron con un ELISA y un antígeno recombinante de superficie de F. hepatica (FhrAPS). Los datos obtenidos se analizaron de acuerdo con la raza, edad sexo y aptitud. La seroprevalencia global en Uruguay resultó del 56% (Intervalo de Co...

  4. AGREGANDO VALORES A LAS CADENAS DE VALOR

    Directory of Open Access Journals (Sweden)

    Gregory J. Scott

    2014-01-01

    Full Text Available Este artículo presenta una matriz de factores a tomar en cuenta para priorizar las cadenas de valor agrícolas seleccionadas como foco de las iniciativas público-privadas para impulsar su desarrollo. Dichos factores dan especial importancia a temas de responsabilidad social empresarial. Se ilustra su uso con datos y una revisión de la literatura sobre Perú.

  5. Frequency of human toxocariasis in a rural population from Cajamarca, Peru determined by DOT-ELISA test Freqüência de toxocaríase humana numa população rural de Cajamarca, Peru, mediante o uso do teste DOT-ELISA

    Directory of Open Access Journals (Sweden)

    William H. Roldán

    2009-04-01

    Full Text Available The aim of this study was to estimate the frequency of human toxocariasis in Cauday district, Cajamarca, Peru, using a dot-ELISA test. From June to October 2005, a total of 256 adult subjects were studied. Blood samples were collected for serology by a dot-ELISA test and for hematological examination. Parasitological examination was also carried out in stool samples to check cross-reactions in the dot-ELISA. The frequency observed was 44.92%, with a significant higher proportion of positivity in male subjects. From subjects with positive serology, 45.6% had respiratory symptoms, 40.44% abdominal pain, 32.35% hepatic symptoms, 14.7% cutaneous signs, 13.23% ocular manifestations, 43.38% eosinophilia, and all of these were statistically associated to serology. Among the population evaluated, 90.23% (231/256 were parasitized. From subjects with positive serology, 92.17% had at least one intestinal parasite and the most frequent were: Blastocystis hominis (68.38%, Giardia lamblia (28.68%, Hymenolepis nana (20.0%, Ascaris lumbricoides (15.65%, Entamoeba histolytica/E. dispar (13.24%, Cyclospora cayetanensis (4.41%, Cryptosporidium sp. (1.47%, Enterobius vermicularis (0.87%, Strongyloides stercoralis (0.87%, Taenia sp. (0.87%, and Trichuris trichiura (0.87%. The rate of false positives in the dot-ELISA test was improved by serum absorption each with A. suum antigens, with a decrease of cross-reactions. In conclusion, human toxocariasis is highly frequent in this population and some risk factors like dog/cat ownership, presence of pets within house, and previous history of geophagia were observed in the present study.O propósito do presente estudo foi estimar a freqüência da toxocaríase no distrito de Cauday, Cajamarca, Peru, usando o dot-ELISA teste. Entre junho e outubro de 2005, um total de 256 pessoas foram avaliadas. Coletaram-se amostras de sangue para o teste de dot-ELISA e para o exame hematológico e amostras de fezes para exame parasitol

  6. Diagnosis of amebic liver abscess by means of an anzyme-linked immunoabsorbent assay Diagnóstico de absceso hepático amibiano mediante el inmunoensayo enzimático ligado a una enzima (ELISA

    Directory of Open Access Journals (Sweden)

    Jorge Humberto Botero Garcés

    2001-02-01

    Full Text Available Amebic liver abscess (ALA is the most frequent extraintestinal complication of amibiasis; its clinical presentation is generally acute, and it is considered a medical emergency. Patients manifest general symptoms, accompanied by pain in the right hypochondrium that can irradiate to the shoulder; painful hepatomegalia is a suggestive but not diagnostic sign; it should be distinguished from pyogenic liver abscess and necrotic hepatoma; for the diagnostic approach to ALA imagenologic exams and laboratory tests are required, among the latter the immunologic ones. In this study we used the ELISA test for the determination of antiamebic antibodies in control ALA sera, as described by the National Institute of Health Bogotá, Colombia (7. Furthermore, 67 serum samples were evaluated from patients with clinic suspicion of ALA. Of these 70.2 were positive; there was significant difference in the positivity rate between patients from two Colombia cities, those from Bucaramanga having a higher positivity rate. El absceso hepático amibiano (aha es la complicación extraintestinal más frecuente de la amibiasis; su cuadro clínico es generalmente agudo y se lo considera una urgencia médica. Se manifiesta con síntomas generales, acompañado de dolor en hipocondrio derecho, que se puede irradiar al hombro; la hepatomegalia dolorosa es un signo sugestivo pero no diagnóstico; debe diferenciarse del absceso hepático piógeno y del hepatoma necrótico; para el enfoque diagnóstico se requieren ayudas imaginológicas y pruebas de laboratorio como las inmunológicas. En este estudio se puso a punto la prueba de ELISA para la determinación de anticuerpos antiamibianos en sueros controles de pacientes con AHA, estandarizada en el Instituto Nacional de Salud de Bogotá. Además se evaluaron 67 muestras de pacientes con sospecha clínica de AHA, procedentes del Hospital González Valencia de Bucaramanga y del Hospital Universitario San Vicente de Paúl de Medell

  7. Cadenas productivas y productividad de las Mipymes

    OpenAIRE

    Danielle Tomta; Cèsaire Chiatchoua

    2010-01-01

    ResumenEl concepto de las cadenas productivas ha tomado en los últimos años un peso importante para el desarrollo de las empresas, tanto en los países desarrollados como en desarrollo. Sin embargo, en los países en desarrollo, los principales agentes económicos que son las empresas no han sabido convertir este elemento en una ventaja competitiva. Peor aún en México donde la política económica no ha sido diseñada de acuerdo a las necesidades del sector productivo, mismo que se refleja por el b...

  8. RESEÑA DEL SOFTWARE DISPONIBLE EN COLOMBIA PARA LA GESTIÓN DE INVENTARIOS EN CADENAS DE ABASTECIMIENTO

    OpenAIRE

    VALENTINA GUTIÉRREZ; DIANA PATRICIA JARAMILLO

    2009-01-01

    La gestión de los diferentes tipos de inventario a lo largo de una cadena de abastecimiento es un problema complejo que, en la mayoría de los casos de la industria colombiana e internacional, se aborda mediante la implementación de herramientas de software. Sin embargo, la decisión de implementar dichas herramientas está en muchos casos limitada por la capacidad financiera para invertir en ellas y por el desconocimiento de la oferta de software disponible en Colombia. Este artículo presenta u...

  9. El estudio de la cadena productiva del fique

    Directory of Open Access Journals (Sweden)

    Maria Eugenia Morales Rubiano

    2002-09-01

    Full Text Available El estudio recopila los resultados más importantes obtenidos en el trabajo de grado denominado "Estudio de factibilidad de la cadena productiva del fique cultivado en Colombia", el cual tomo como punto de referencia el sector fiquero para estimas la factibilidad de aplicar un esquema de cadena productiva, a través de la estructura y análisis estratégico del sistema.

  10. Análisis de comportamiento caótico en variables de la cadena de suministro

    Directory of Open Access Journals (Sweden)

    Sergio A. Ramírez

    2011-12-01

    Full Text Available Este artículo desarrolla un modelo de cadena de suministro de cuatro niveles mediante la utilización de la dinámica de sistemas con el software Ithink® y Mathlab® para el análisis de los datos. La cadena de suministro se estudia a partir de la Teoría del Caos, teoría que ayuda a identificar cuáles son las variables más sensibles que puedan llevar a un estado de caos. Se construye un modelo de estructura genérica que comprende los eslabones de fábrica, distribución, mayorista, minorista y cliente. Se simula la dificultad que los gerentes de operaciones y logística encuentran para decidir sobre cuánto ordenar y en qué momento, de acuerdo con lo que ocurre en los eslabones del nivel anterior y posterior, mediante dos parámetros de decisión: el primer parámetro denominado A indica la participación de la cantidad de unidades (entre 0 y 1 que se tiene en cuenta de los productos en el inventario actual y los pedidos pendientes, al momento de hacer el pedido. El segundo parámetro denominado B es la participación de la cantidad de unidades (entre 0 y 1 que se encuentran en la línea de suministro (en tránsito al momento de hacer el pedido en cada uno de los eslabones de la cadena de suministros.

  11. INMUNODIAGNOSTICO DE FASCIOLOSIS BOVINA MEDIANTE ELISA Y WESTERN BLOT

    OpenAIRE

    Texia Gorman; Roxana Sanchez; Fernando Fredes; Hector Alcaino

    1998-01-01

    Se evaluó y caracterizó la respuesta inmune humoral de bovinos naturalmente infectados con Fasciola hepatica frente a un extracto total de antígenos de excreción-secreción (E-S) y a una fracción antigénica semipurificada cromatográficamente (

  12. Decisiones en la gerencia de la cadena de suministro

    OpenAIRE

    DRANGO SERNA, MARTÍN D.; PÉREZ ORTEGA, GIOVANNI; ARANGO MARTÍNEZ, CARLOS A.

    2009-01-01

    Las presiones y desafíos de la globalización han llevado a las organizaciones de hoy a ser cada vez más competitivas si quieren sobrevivir en este ambiente. En ese afán de mejorar la competitividad, las organizaciones tratan de alinear sus objetivos e intereses con otras compañías en lo que se conoce como la cadena de suministro. Esa alineación de objetivos no es fácil, surge entonces la gerencia de la cadena de suministro, que pretende la integración de los procesos claves de las organizacio...

  13. Análisis FODA de la cadena arrocera del Estado Portuguesa considerando los procesos de integración con el Mercosur y la CAN

    OpenAIRE

    Vivas Sánchez, Víctor Vicente; Albisu Aguado, Luis Miguel

    2010-01-01

    La cadena arrocera del Estado Portuguesa, posee condiciones especiales para el desarrollo del cultivo y del proceso agroindustrial consiguiente, tales como, elevado potencial de tierras con condiciones para la producción de este cereal, incrementos en producción obtenidos mediante mejoramiento de las técnicas de manejo y fitomejoramiento, productores con un elevado nivel técnico y adecuada infraestructura productiva, así como presencia de un liderazgo real y de organizaciones gremiales sólida...

  14. OPTIMIZACIÓN DE LOS NIVELES DE INVENTARIO EN UNA CADENA DE SUMINISTRO / INVENTORY LEVELS OPTIMIZATION IN A SUPPLY CHAIN

    Directory of Open Access Journals (Sweden)

    José Antonio Díaz-Batista

    2012-04-01

    Full Text Available La gestión de inventarios es un tema muy estudiado por la Gestión de Operaciones y la Investigación de Operaciones para optimizar el costo de la gestión de los inventarios. Los modelos de inventario tradicionales generalmente trataban la optimización de inventarios para una organización individual. Pero recientemente ha comenzado a imponerse en la gestión empresarial el concepto de “cadena de suministro”, donde la satisfacción de las necesidades del cliente final se logra mediante los flujos material, de información y financiero, que se establecen desde los proveedores originales hasta el último consumidor, requiriendo esto de un determinado nivel de cooperación e integración entre los participantes en la cadena. En este trabajo se estudia la optimización de los inventarios conjuntos suministrador-comprador, comparándolos con las políticas tradicionales no colaborativas, analizándose, como conclusión, las ventajas de este enfoque en los costos totales de inventario en la cadena.AbstractInventory management is a strongly observed subject in Operations Management and Operations Research, seeking for inventory management cost optimization. Traditional inventory models mainly deal with optimization inside a single enterprise. Yet, actually the supply chain concept has begun to overcome others approaches in the business management area. Supply chain management seeks customer satisfaction through material, financial and informational flows that are establish throughout the chain, from suppliers until the final consumer. This requires certain level of coordination and integration among supply chain members. This paper studies join supplier-buyer inventory optimization, comparing them with traditional non-collaborative policies, and analyzing advantages of this collaborative approach in the total inventory costs of the supply chain.

  15. Dynex: multiplex ELISA technology

    Science.gov (United States)

    Conventional enzyme linked immunosorbent assay (ELISA) is a gold standard for screening antibodies and testing for protein or antigen presence. A significant limitation of this assay resides in the fact that only one analyte can be assessed per microplate well. Here, we describe and investigate a ne...

  16. Optimización de una cadena de suministro

    OpenAIRE

    Antúnez Ruiz, Marta

    2006-01-01

    El presente proyecto se ha realizado bajo un convenio de cooperación educativa entre Universidad y Empresa en una planta de productos químicos de una compañía de gran consumo. El proyecto se basa en la optimización de una cadena de suministro a través de la eliminación de las pérdidas existentes, entendiendo como pérdidas todas las operaciones que no dan valor añadido al producto final. La metodología usada es el Value Stream Mapping, una herramienta del Lean Manufacturing. La ...

  17. Cambio de concepto de Compras a Cadena de Suministros

    OpenAIRE

    Rafael Nogales González

    2007-01-01

    Se expone la necesidad de cambiar el concepto de compras a una dimensión mayor e incluida en una gestión más eficiente. Se presenta un marco teórico de las principales funciones del área de aseguramientos así como inicios de la Logística en la gestión empresarial. Se estudian los métodos de aprovisionamiento, las principales funciones del departamento de compras y como éste se integra en la nueva filosofía de la Cadena de Suministros. Se exponen las ideas principales del Sistema de Gestió...

  18. Cadenas productivas lácteos y café

    OpenAIRE

    Giraldo Molina, Clara Inés

    2012-01-01

    Este cuaderno es producto de una investigación, en torno a las industrias de lácteos y café. El texto presenta una caracterización de los dos sectores productivos teniendo en cuenta las características de sus cadenas de producción, con el objetivo de describir los procesos, subproductos generados, infraestructura, innovaciones y necesidades correspondientes. Todo esto con el fin de lograr mayor productividad y competitividad de las industrias de lácteos y café a nivel nacional e internacional...

  19. Gestión por competencias para organizaciones sin ánimo de lucro vinculadas a la Cadena láctea Programa PAAP, Ministerio de Agricultura y Desarrollo Rural en Boyacá

    OpenAIRE

    Medina Castañeda, Nancy Aidé

    2014-01-01

    Los objetivos del trabajo final son proponer la aplicación de la gestión por competencias en organizaciones sin ánimo de lucro de la cadena láctea en el programa Apoyo Alianzas Productivas del Ministerio de Agri-cultura y Desarrollo Rural, en Boyacá; mediante el desarrollo de un marco de referencia teórico, la deter-minación del estado y el diseño de una metodología de aplicación. Los resultados obtenidos de un análisis cualitativo-cuantitativo en 14 ESAL de los municipios de Tinjaca, Nobsa, ...

  20. Políticas para la inserción de las microempresas y las pequeñas y medianas empresas en cadenas globales de valor en América Latina

    OpenAIRE

    Stezano, Federico

    2013-01-01

    En el presente documento se describe un marco conceptual de las políticas de apoyo a las microempresas y las pequeñas y medianas empresas (mipymes) basado en una revisión detallada de obras teóricas y empíricas sobre este tema, poniendo un énfasis particular en los instrumentos de política que facilitan la inserción de este grupo de empresas en cadenas mundiales de valor y su internacionalización mediante diversos procesos de innovación tecnológica y modernización de las organizaciones. Los b...

  1. Similitud melódica como transformación de cadenas I.

    OpenAIRE

    Gomez Martín, Francisco

    2013-01-01

    He aquı una nueva serie de tres artıculos que investigan desde un punto de vista divulgativo la relacion entre la similitud melódica y la teoría de cadenas (una rama de la computación). La similitud melódica, bajo ciertas hipótesis que simplifican el complejo fenómeno que es, se puede con- cebir como un problema de comparación de cadenas, donde las cadenas aquí representan sucesiones de notas. En un artículo titulado Comparison of musical sequences Mongeau y Sankoff [MS90]explotaron es...

  2. Generando entrelazamiento en cadenas XY - (Generating entanglement in XY chains)

    CERN Document Server

    Schmiegelow, C T

    2006-01-01

    Se estudia en este trabajo la capacidad de generar entrelazamiento de una cadena de espines con acoplamiento de Heisenberg XY y un campo magnetico uniforme a partir de un estado inicial en el que los espines estan completamente alineados. Se encuentra que la capacidad de generar estados entrelazados no muestra un comportamiento monotono con el campo presentando, en cambio, plateaus y resonancias. Tambien se muestra que, a pesar de que la anisotropia es necesaria para que se generen estados entrelazados, una mayor anisotropia no implica necesariamente mejores condiciones para generar entrelazamiento que sirva para usarse en una computadora cuantica. Inclusive, se observa que, se genera una cantidad finita de entrelazamiento en el limite de pequena anisotropia. (The maximum entanglement reached by an initially fully aligned state evolving in a XY Heisenberg spin chain placed in a uniform transverse magnetic field is studied. It is shown that the capacity to create entangled states (both of one qubit with the re...

  3. La cadena de valor del sector confección

    Directory of Open Access Journals (Sweden)

    Maryana Sandrea

    2004-01-01

    Full Text Available El alto grado de competitividad empresarial sumado a los efectos del fenómeno de la globalización, abren nuevos retos y oportunidades para las empresas locales. Dentro de este contexto, el presente artículo tiene como objetivo identificar los componentes cualitativos de la cadena de valor para el producto líder del sector confección en el municipio Maracaibo del estado Zulia (Venezuela; por medio de un estudio de tipo descriptivo-transeccional, utilizando entrevistas semiestructuradas. De acuerdo a los resultados obtenidos las franelas constituyen el producto líder para la mayoría de las empresas entrevistadas. La construcción de la cadena de valor, mostró que la mayor parte de los gerentes del sector (72,73% para 1999 y 66,67% para el 2003 consideraban a la diferenciación del producto como el factor determinante en la decisión de compra del cliente. Al mismo tiempo, un 54,55% en 1999 y un 81,82% en el 2003, identificó a la producción como la etapa clave en la generación de valor. Por lo que, se estiman como fundamentales las actividades primarias de operaciones y logística de entrada, así como las actividades de apoyo relativas al recurso humano, y al desarrollo de tecnologías. Finalmente, el estudio plantea como fuentes potenciales de ventajas competitivas, la optimización de actividades internas y el análisis de los nexos con proveedores, pudiendo aplicarse una estrategia competitiva de diferenciación con minimización del impacto en costos.

  4. Estrategias de nivel simple en cadenas de suministro de repuestos

    Directory of Open Access Journals (Sweden)

    Oswaldo Moscoso Zea

    2013-12-01

    Full Text Available (Recibido: 2013/09/30 - Aceptado: 2013/12/02La Gestión de Inventarios en Cadenas de Suministro de Repuestos (GICSR juega un rol importante tanto para la Compañía Cliente que desea mantenerse operativa a pesar de fallas en la maquinaria o equipos y la Compañía Proveedora o Productora que desea mejorar sus procesos de servicios de post venta. El incremento en la importancia de GICSR requiere la búsqueda de la mejora e innovación de las estrategias en esta área. Este artículo describe estrategias de Nivel Simple de gestión de inventarios en prácticas de administración de repuestos y sirve como una fuente para decidir sobre la mejor estrategia en función del tipo de negocio y la aplicación. Hoy en día estas prácticas no son solo una obligación legal de una organización sino también una oportunidad de construir lealtad y relaciones comerciales a largo plazo con los clientes.

  5. Herramientas computacionales avanzadas para la gestión integrada de la cadena de suministro

    OpenAIRE

    Galli, María Rosa; Chiotti, Omar Juan Alfredo

    2001-01-01

    La gestión integrada de la cadena de suministro no puede sustentarse en la relación jerárquica convencional e implica una nueva forma de relación estratégica basada en un modelo de colaboración entre las empresas. Este modelo de colaboración implica rediseñar los mecanismos y las técnicas de gestión de la cadena de suministro interna de cada empresa, con la visión de dar soporte a los procesos de colaboración y negociación que la misma debe mantener con las otras entidades de la cadena de sum...

  6. Cadenas de rodillos. Tendencias de desarrollo y dimensionales según normas ISO.

    Directory of Open Access Journals (Sweden)

    M. E. García Domínguez

    2007-05-01

    Full Text Available El artículo es una compilación de resultados derivados de un estudio del desarrollo de la transmisión por cadena de rodillos que permite brindar información relevante sobre la tendencia del mercado internacional, innovaciones recientes en las cadenas de rodillos y las diferencias dimensionales que caracterizan las series geométricas de las cadenas ISO-A e ISO-B según la norma ISO 606:2004The paper is a compilation of some results associated with the development of roller chain transmissions and outstanding information in relations with the roller chain market trend, recent innovations and dimensional differences of ISO-A and ISO-B roller chain series according to ISO Standard 606:2004.

  7. A quantitative ELISA for dystrophin.

    Science.gov (United States)

    Morris, G E; Ellis, J M; Nguyen, T M

    1993-05-01

    A novel approach to the quantitation of the muscular dystrophy protein, dystrophin, in muscle extracts is described. The two-site ELISA uses two monoclonal antibodies against dystrophin epitopes which lie close together in the rod domain of the dystrophin molecule in order to minimize the effects of dystrophin degradation. Dystrophin is assayed in its native form by extracting with non-ionic detergents and avoiding the use of SDS. PMID:8486926

  8. Inventarios colaborativos en la optimización de la cadena de suministros

    OpenAIRE

    Martin Dario Arango-Serna; Wilson Adarme-Jaimes; Julian Andres Zapata-Cortes

    2013-01-01

    La colaboración entre las partes de la cadena de suministro es una de las áreas de estudio más prometedoras, tanto para el entorno académico como para el empresarial, impulsada por los benefi cios que las empresas y las cadenas de suministro pueden obtener a partir de esta. Este artículo presenta una revisión de la colaboración en el manejo de inventarios, la cual es una de las estrategias más importantes para intentar reducir costos, mejorar el nivel de servicio y satisfacer a los clientes. ...

  9. La Cadena de Suministro de la Energía Solar

    OpenAIRE

    Virginia Guadalupe López Torres; Carmen Alcalá Álvarez; Luis Ramón Moreno Moreno

    2012-01-01

    Se presenta la cadena de suministro instalada en México para el mercado de la energía solar, con base a una investigación exploratoria realizada en bases de datos especializadas de empresas dedicadas a la fabricación, proveeduría, comercialización y prestación de servicios de sistemas solares. Los resultados indican la no existencia en México de una cadena de suministro articulada, sólo se perciben eslabones aislados, por ello se plantea que el gobierno a través de políticas públicas fomente ...

  10. Enverdecimiento de la cadena de abastecimiento en las empresas manufactureras bogotanas

    OpenAIRE

    Velásquez Rodríguez, Oscar Fabián

    2012-01-01

    El presente trabajo busca estudiar los efectos sinérgicos existentes entre la manufactura esbelta y las prácticas de enverdecimiento de la cadena de abastecimiento, así como los posibles beneficios que dicha interacción puede generar en el desempeño económico y ambiental de las empresas. De acuerdo con esto, se plateó un modelo de moderación en el cual las prácticas de manufactura esbelta potencializan los efectos positivos que el enverdecimiento de la cadena de abastecimiento puede producir ...

  11. Empresas y territorio: Cadenas productivas, redes, innovación y competitividad

    OpenAIRE

    Carlo Pietrobelli

    2009-01-01

    Esta presentación ofrece una revisión de los asuntos relacionados con cadenas de valor y la productividad de empresas. El documento explica conceptos como innovación, "upgrading", competición, organización de negocios, oportunidades y rentas. Utilizando un análisis de varias teorías y casos de producción específicos, la presentación indica algunos resultados. Se muestra que la eficiencia económica colectiva ayuda al proceso de upgrading y cómo el modelo de governance de cadenas afecta el upgr...

  12. RESEÑA DEL SOFTWARE DISPONIBLE EN COLOMBIA PARA LA GESTIÓN DE INVENTARIOS EN CADENAS DE ABASTECIMIENTO

    Directory of Open Access Journals (Sweden)

    VALENTINA GUTIÉRREZ

    2009-01-01

    Full Text Available La gestión de los diferentes tipos de inventario a lo largo de una cadena de abastecimiento es un problema complejo que, en la mayoría de los casos de la industria colombiana e internacional, se aborda mediante la implementación de herramientas de software. Sin embargo, la decisión de implementar dichas herramientas está en muchos casos limitada por la capacidad financiera para invertir en ellas y por el desconocimiento de la oferta de software disponible en Colombia. Este artículo presenta una reseña del software disponible en Colombia para la gestión de inventarios con el fin de brindar a las empresas que se enfrentan a la decisión de implementar un software, una herramienta de soporte que indique cuál es la oferta en Colombia y cuáles son las características de los sistemas disponibles. Para esto se presenta primero el estado del arte, en el cual se identifica el resultado de la revisión de la literatura y el uso de las herramientas de software en la industria colombiana. Seguidamente se presenta la caracterización de los productos de software disponibles en el país. Dicha caracterización permite identificar la configuración general de los productos, los módulos de gestión con los que cuenta, las opciones de integración con otras herramientas informáticas, y la información general de precios e instalación. Finalmente se presentan las conclusiones del estudio y las futuras oportunidades de investigación.

  13. Disseny i implementació del data warehouse d'una cadena de botigues de roba

    OpenAIRE

    Díaz Muñoz, Ricardo

    2016-01-01

    Este documento describe el diseño y la construcción del prototipo de un sistema de base de datos relacional de acuerdo con las especificaciones y requerimientos recibidos, que podría ser una primera versión de un sistema data warehouse para una cadena de tiendas de ropa.

  14. Evaluación de la influencia de la cadena de frio en la productividad y competitividad de la cadena de suministro de la mora de castilla (rubus glaucus benth.)- estudio de caso departamento del Huila

    OpenAIRE

    Polanía Orozco, Sebastián de Jesús

    2012-01-01

    La presente investigación inicia con una descripción del contexto de la cadena de suministro de la Mora de Castilla y la Cadena de Frio Aplicada a los alimentos; los principales elementos que dirigen el comportamiento y la interacción de dichas cadenas, realizando un especial énfasis en los factores críticos en el desarrollo de la competitividad y productividad del sector. Luego se desarrollan los principales elementos para la estructuración en el departamento del Huila, determ...

  15. Microestructura y composición mineral del yunque normal y patológico : estudio mediante ftir y sem-edx. 645763563

    OpenAIRE

    Herrero Calvo, David

    2016-01-01

    El yunque es el huesecillo más grande de la cadena osicular humana. La patología crónica del oído medio puede provocar alteraciones en la estructura del yunque ocasionando la pérdida de su función y originando una disminución de las habilidades auditivas del paciente. La espectroscopía de infrarrojos (FTIR) y la microscopía electrónica de barrido (SEM-EDX) permiten obtener información sobre la composición de la muestra y evaluar su microarquitectura. Se han analizado 71 yunques mediante FTIR,...

  16. ¿Cómo aplicar la flexibilidad administrativa en las entidades de educación superior mediante el sistema de costos abc?

    OpenAIRE

    Dilia Castillo

    2005-01-01

    El método de Costos Basado en Actividades se ha creado para generar en las organizaciones, estructuras administrativas flexibles mediante la clasificación de las actividades (cadena valor), en centros primarios y de apoyo. Este sistema permite la acumulación de costos en forma directa en cada dependencia responsable de desarrollar tareas que enconjunto originan que una organización ofrezca a sus clientes un producto terminado o un servicio eficiente a un costo real previamente determinado. La...

  17. Efecto de las decisiones de pedidos de clientes mayoristas en cadenas de abastecimiento: Un análisis experimental.

    OpenAIRE

    Villa Betancur, Sebastián

    2011-01-01

    Uno de los problemas más conocidos y costosos en las Cadenas de Abastecimiento se puede presentar ante la escasez de un producto en algún eslabón de la cadena. Este problema es caracterizado por el aumento de la variabilidad en las órdenes a medida que éstas se mueven a lo largo de la cadena. En esta tesis se investiga la forma en que un cliente intermedio (mayorista), enfrentando aumentos en la demanda del cliente final (minoristas), realiza sus pedidos a su único proveedor. Este proveedor c...

  18. La calidad ambiental como variable estratégica en cadenas agroalimentarias con tecnologías continuas

    OpenAIRE

    Jiménez, Gerardo; Pelupessy, Wim

    2009-01-01

    El objetivo de este artículo es desarrollar una metodología que permita evaluar las ventajas del mejoramiento estratégico de la calidad ambiental a lo largo de las cadenas agroalimentarias con tecnologías continuas. El desarrollo metodológico se lleva a cabo complementando el enfoque de las Cadenas Globales de Mercancías (CGM) con la teoría de juegos. En el artículo se hace una aplicación a la cadena global del café de Costa Rica. Específicamente el análisis se concentra en las potencialidade...

  19. Coordinación de agentes en cadenas de suministro descentralizadas, caso de estudio sector panificador, Palmira, Valle

    OpenAIRE

    Cárdenas Barbosa, Iván Darío

    2013-01-01

    En la presente tesis de maestría se desarrolla una propuesta metodológica para el modelado de cadenas de suministro desde un enfoque en el cual se consideren aspectos que tradicionalmente no se tienen en cuenta y que cobran mayor relevancia en un entorno de pequeñas empresas: reglas de negociación entre actores de la cadena, factores humanos presentes y la relevancia de la experiencia adquirida. El desarrollo de este proyecto incluye caracterización de una cadena de suministro del sector pani...

  20. Gobernanza en las Cadenas Globales de mercancías/valor: una revisión conceptual

    OpenAIRE

    Rafael Díaz Porras; Jorge Andrey Valenciano Salazar

    2012-01-01

    ResumenEn este documento se analiza el sistema de gobernanza de las cadenas de mercancías/valor a efectos de establecer los elementos conceptuales que se requieren para su análisis y derivar consecuencias analíticas para estudios que se realicen con la intención de mejorar la competitividad de actores participantes en los segmentos periféricos de las cadenas, desde países en vías de desarrollo. El análisis se efectúa siguiendo el enfoque de cadenas globales de mercancías y su evolución hacia ...

  1. Rearreglos de genes de cadenas pesadas de las inmunoglobulinas en las gammapatías monoclonales Immunoglobulin heavy chain gene rearrangements in the monoclonal gammopathies

    Directory of Open Access Journals (Sweden)

    Andrea Bosaleh

    2005-06-01

    Full Text Available Las neoplasias de células plasmáticas resultan de la expansión de un clon de células B que secreta inmunoglobulinas, conocido como componente monoclonal o componente M. Las neoplasias malignas incluyen al mieloma múltiple y la macroglobulinemia de Waldenström, y la condición premaligna comprende las gammapatías monoclonales de significado incierto (MGUS. El MGUS presenta un componente monoclonal sin evidencia de mieloma múltiple, macroglobulinemia de Waldenström, amiloidosis primaria u otros desórdenes. El diagnóstico se basa en la combinación de características patológicas, radiológicas y clínicas. Aproximadamente el 25% de las gammapatías monoclonales de significado incierto desarrollarán mieloma múltiple, amiloidosis sistémica, macroglobulinemia o enfermedades linfoproliferativas malignas, indicando que sería una condición premielomatosa. El objetivo del presente trabajo es establecer la utilidad clínica de la inmunofenotipificación por citometría de flujo (CF y la detección de clonalidad por biología molecular. Se estudiaron 32 pacientes, siete con diagnóstico de mieloma múltiple y veinticinco con gammapatía monoclonal en estudio, los cuales fueron divididos en cuatro grupos basados en los datos clínicos y los resultados de CF. En el grupo de pacientes con CF no diagnóstica, se realizó la detección de los rearreglos de los genes de las cadenas pesadas de las inmunoglobulinas mediante reacción en cadena de la polimerasa (PCR, detectándose monoclonalidad en el 59% de los casos. El estudio de los rearreglos de los genes de las cadenas pesadas de las IgH mediante PCR incrementa la sensibilidad de detección de monoclonalidad.Plasma cell neoplasia occurs as a result of the expansion of an immunoglobulin-secreting B-cells clones, known as monoclonal component or M component. Malignant neoplasias include multiple myeloma and Waldenström macroglobulinemia, while premalignant conditions comprise monoclonal

  2. LA POLÍTICA DE DEVOLUCIÓNES EN LAS GRANDES CADENAS DE DISTRIBUCIÓN PRESENTES EN SAN JOSÉ DE CÚCUTA COMO MODELO DE FIDELIZACIÓN DEL CLIENTE

    Directory of Open Access Journals (Sweden)

    Saúl Villalobos Marín

    2016-02-01

    Full Text Available Resumen La presente investigación tiene como objetivo determinar la política de devoluciones en las principales cadenas de distribución presentes en la ciudad de San José de Cúcuta como estrategia de fidelización del cliente y su implementación, mediante la creación de un modelo para las pequeñas y medianas empresas de la ciudad. Para dar el cumplimiento a este propósito, se realizó un estudio explicativo ya que no sólo persigue describir o acercarse al problema, sino que intenta encontrar las causas del mismo y proponer una posible estrategia para su solución. Para ello se realizó un estudio a todo el proceso que involucra la gestión de las devoluciones para analizar el contexto de esta estrategia en las principales cadenas de distribución de la ciudad. El estudio se realizó mediante una encuesta y entrevista personal a los responsables de las políticas de fidelización y/o devoluciones. Creándose posteriormente un modelo de política de devoluciones basado en las conclusiones extraídas y teniendo en cuenta los antecedentes nacionales e internacionales. Se evidenció la relación entre la política de devoluciones y la fidelización del cliente, se pudo reflejar la opinión altamente positiva que tienen los responsables de los establecimientos de este tipo de estrategias. Se dejó patente que las empresas que aplican una estrategia enfocada a la satisfacción del cliente obtienen mejores resultados en ventas y crecimiento empresarial respecto a las grandes cadenas que despreocupan esta parte. Se pudo mostrar como las estadísticas y la opinión de los gerentes demuestra que es rentable para la empresa en términos económicos, la aplicación de este tipo de políticas y como una empresa con una política de devoluciones clara y bien definida sale beneficiada y reforzada en su imagen de marca, percepción de los clientes y estabilidad en las ventas para poder afrontar periodos económicos adversos como crisis económicas o

  3. Gobernanza en las Cadenas Globales de mercancías/valor: una revisión conceptual

    Directory of Open Access Journals (Sweden)

    Rafael Díaz Porras

    2012-07-01

    Full Text Available ResumenEn este documento se analiza el sistema de gobernanza de las cadenas de mercancías/valor a efectos de establecer los elementos conceptuales que se requieren para su análisis y derivar consecuencias analíticas para estudios que se realicen con la intención de mejorar la competitividad de actores participantes en los segmentos periféricos de las cadenas, desde países en vías de desarrollo. El análisis se efectúa siguiendo el enfoque de cadenas globales de mercancías y su evolución hacia el concepto de cadenas de valor. Palabras claves: Cadenas de valor, cadenas globales de mercancías, gobernanza, competitividad. AbstractThis paper analyzes the governance system of commodity chains in order to establish the conceptual elements that are required for the compression and analysis of this methodology. In addition, some analytical implications are proposed for studies intended to improve the competitiveness of actors participating in the peripheral segments of the chains from developing countries. The analysis has been conducted under the focus of global commodity chains and their evolution towards the concept of value chains. Keywords: Value chains, global commodity chains, governance, competitiveness.

  4. La Inteligencia Institucional y Cadena de Valor de la Información: El Ejemplo de la Universidad Federal de Rio Grande do Norte - Brasil

    Directory of Open Access Journals (Sweden)

    Márcia Josienne Monteiro Chacon

    2014-12-01

    Full Text Available La transformación del progreso científico y tecnológico lleva a la rápida difusión de conocimientos, es la era de los datos. La inteligencia de negocios tiene cómo objetivos recoger datos para la toma de decisiones. El término Inteligencia Institucional es adecuado para su uso en la Universidad. La realización de la cadena de valor son las unidades administrativas. Así, los problemas que amenazan en los servicios de TI, tiene su origen en la aplicación inadecuada. En los aspectos de la gestión no se puede olvidar que los usuarios finales, puesto que mediante el uso efectivo de los productos de datos pueden generar valor para la Universidad. Se sabe que hay limitaciones y el impacto que pueden tener en la iniciativa tiende a bajar. Recomendamos más investigaciones porque no es nuestra intención de agotar el tema, sino presentar algunos conceptos que impulsan el proceso.

  5. La Inteligencia Institucional y Cadena de Valor de la Información: El Ejemplo de la Universidad Federal de Rio Grande do Norte - Brasil

    Directory of Open Access Journals (Sweden)

    Márcia Josienne Monteiro Chacon

    2014-11-01

    Full Text Available La transformación del progreso científico y tecnológico lleva a la rápida difusión de conocimientos, es la era de los datos. La inteligencia de negocios tiene cómo objetivos recoger datos para la toma de decisiones. El término Inteligencia Institucional es adecuado para su uso en la Universidad. La realización de la cadena de valor son las unidades administrativas. Así, los problemas que amenazan en los servicios de TI, tiene su origen en la aplicación inadecuada. En los aspectos de la gestión no se puede olvidar que los usuarios finales, puesto que mediante el uso efectivo de los productos de datos pueden generar valor para la Universidad. Se sabe que hay limitaciones y el impacto que pueden tener en la iniciativa tiende a bajar. Recomendamos más investigaciones porque no es nuestra intención de agotar el tema, sino presentar algunos conceptos que impulsan el proceso.

  6. Hyperglucagonaemia analysed by glucagon sandwich ELISA

    DEFF Research Database (Denmark)

    Albrechtsen, Nicolai Jacob Wewer; Hartmann, Bolette; Veedfald, Simon; Windeløv, Johanne A; Plamboeck, Astrid; Bojsen-Møller, Kirstine N; Idorn, Thomas; Feldt-Rasmussen, Bo; Knop, Filip K; Vilsbøll, Tina; Madsbad, Sten; Deacon, Carolyn F; Holst, Jens J

    2014-01-01

    the extent to which the hyperglucagonaemia measured in clinical samples was caused by authentic glucagon. METHODS: We examined the performance of three commercial glucagon 'sandwich' ELISAs. The ELISA with the best overall performance was selected to compare glucagon measurements in clinical samples...

  7. Inventarios colaborativos en la optimización de la cadena de suministros

    Directory of Open Access Journals (Sweden)

    Martin Dario Arango-Serna

    2013-01-01

    Full Text Available La colaboración entre las partes de la cadena de suministro es una de las áreas de estudio más prometedoras, tanto para el entorno académico como para el empresarial, impulsada por los benefi cios que las empresas y las cadenas de suministro pueden obtener a partir de esta. Este artículo presenta una revisión de la colaboración en el manejo de inventarios, la cual es una de las estrategias más importantes para intentar reducir costos, mejorar el nivel de servicio y satisfacer a los clientes. El artículo realiza una revisión de los modelos de inventario colaborativos encontrados en la literatura científi ca, analiza los obstáculos mas frecuentes para su aplicación y propone algunas alternativas para eliminar dichos obstáculos.

  8. Talento verde y cadenas de suministro verdes: ¿existe una relación significativa?

    Directory of Open Access Journals (Sweden)

    María del Carmen Torres-Salazar

    2016-01-01

    Full Text Available Introducción: En este texto se presentan resultados estadísticos de la relación entre directivos con características de talento verde y la implementación de prácticas de cadena de suministro verde. Debido a que existen investigaciones sobre la importancia de la visión de la alta dirección para la adopción de prácticas verdes; y sin embargo ninguna da cuenta de las características personales del directivo, ni tampoco de la relación significativa entre estas características y la puesta en marcha de prácticas de cadena de suministro verde, esta es la aportación de la presente investigación. El trabajo se desarrolló en empresas manufactureras con más de diez trabajadores de la zona oriente del estado de Morelos México. La caracterización del término talento verde se reportó en el artículo, Talento verde: caracterización y búsqueda, publicado en el número 14 de esta revista. Método: El método utilizado consistió en la construcción de una encuesta dividida en tres categorías que posteriormente se convirtieron en variables: prácticas de cadena de suministro verde, presiones institucionales y talento verde. Las dos primeras se adaptaron de la encuesta de Zhu y Sarkis (2007 y la tercera surgió de la representación social de talento verde, producto de una fase de esta investigación. La encuesta se validó con un grupo de empresarios miembros de la Cámara Nacional de la Industria de la Transformación (CANACINTRA capítulo Morelos. El instrumento validado se aplicó a 26 directivos de empresas manufactureras con más de diez trabajadores de la zona oriente de Morelos, con el fin de probar la relación entre directivos con características de talento verde y la puesta en marcha de prácticas de cadena de suministro verde. Para ello se utilizaron como herramientas estadísticas la correlación de Pearson y la regresión lineal múltiple. Resultados: Las prácticas de cadena de suministro verde de las empresas

  9. Cadena de suministro en el sector minero como estrategia para su productividad

    OpenAIRE

    CORREA ESPINAL, ALEXANDER; Rodrigo A. Gómez Montoya

    2009-01-01

    Este artículo de revisión bibliográfica busca identificar  la utilización de los modelos de cadena de suministro en el sector minero colombiano y suramericano como medio para aumentar la productividad a través de la revisión de estudios, artículos, libros y revistas. Para esto se parte, de la revisión de las generalidades de la minería en Suramérica y Colombia pasando por la descripción de los conceptos de cadena de suministro y su gestión, incluyendo la revisión de sus aplicaciones en Perú, ...

  10. La Cadena de Suministro de la Energía Solar

    Directory of Open Access Journals (Sweden)

    Virginia Guadalupe López Torres

    2012-01-01

    Full Text Available Se presenta la cadena de suministro instalada en México para el mercado de la energía solar, con base a una investigación exploratoria realizada en bases de datos especializadas de empresas dedicadas a la fabricación, proveeduría, comercialización y prestación de servicios de sistemas solares. Los resultados indican la no existencia en México de una cadena de suministro articulada, sólo se perciben eslabones aislados, por ello se plantea que el gobierno a través de políticas públicas fomente su desarrollo a fin de fortalecer este segmento de mercado.

  11. Biodisponibilidad de los ácidos grasos de cadena corta: mecanismos de absorción

    OpenAIRE

    Ros Berruezo, Gaspar; Martínez Graciá, C.; Valencia Arques, J.A.

    2011-01-01

    Los ácidos grasos de cadena corta (AGCC) son ácidos monocarboxílicos con una cadena de uno a seis átomos de carbono saturados. Son los principales aniones del colon. Se presentan como ácidos libres en el tracto gastrointestinal de los mamíferos, siendo el producto fi nal de la digestión microbiana de los carbohidratos. Los índices de producción microbiana y de absorción son tan altos que los AGCC son la mayor fuente de energía (70-80%) en mamíferos como la vaca. La contribución...

  12. Tegnologías de la información en la cadena de suministro

    OpenAIRE

    ALEXANDER CORREA ESPINAL; RODRIGO ANDRÉS GÓMEZ MONTOYA

    2009-01-01

    Este artículo de revisión bibliográfica busca identificar el estado del arte y la utilización de las Tecnologías de la Información y Comunicaciones (TIC’s) en la cadena de suministro y su nivel de aplicación en la industria colombiana a través del análisis de algunos estudios existentes. Para esto se parte de conceptos generales, hasta llegar a las TIC’s logísticas, las cuales varían según las características y necesidades de la cadena de suministro. A partir de dicha revisión se observó ...

  13. Cadenas de Markov, evaluacion multicriterio y evaluacion multiobjetivo para la modelizacion prospectiva del paisaje

    OpenAIRE

    Paegelow, Martin; Camacho Olmedo, Maria,; Menor Toribio, José

    2003-01-01

    Los SIG incluyen entre sus funciones de analisis aquellas destinadas a la modelizacion espacio-temporal. En este trabajo se presentan los resultados de la aplicacion de un método prospectivo, que combina el analisis de una serie cronologica de usos del suelo (cadenas de markov) con la puesta en relacion de estos usos y un conjunto de variables explicativas (evaluacion multicriterio), para, por ultimo, asignar a cada pixel una categoria de ocupacion del suelo (evaluacion multiobjetivo) en la f...

  14. Modelo de integración de cadenas de suministro colaborativas

    Directory of Open Access Journals (Sweden)

    Horacio Bautista-Santos

    2015-01-01

    Full Text Available Los integrantes estratégicos de las cadenas de suministro colab orativas han buscado realizar acciones conjuntas que les permit an sincronizar los eslabones, para mejorar el ser vicio al cliente y hacer fren te a los retos de competitividad globalizada del entorno actual ; esta tendencia se conoce como integración de la cadena de sumin istro y permite mejorar el desempeño de las empresas en su conjunto. En este t rabajo se presenta un modelo de integración de cadenas de suministro cola borativas considerando las perspe ctivas estratégica, táctica y operativa. Para su implementación se diseñ ó un instrumento de medición, el cual fue validado estadísticamente y se formuló un modelo mate mático utilizando lógica difusa para su resolución. El modelo se aplic ó en 36 empresas mexicanas entre pequeñas, medianas y grandes, determinando su nivel de integración, con base en los resultado s se propusieron acciones específicas a implementar para mejora r el nivel alcanzado.

  15. TEGNOLOGÍAS DE LA INFORMACIÓN EN LA CADENA DE SUMINISTRO

    Directory of Open Access Journals (Sweden)

    ALEXANDER CORREA ESPINAL

    2009-01-01

    Full Text Available Este artículo de revisión bibliográfica busca identificar el estado del arte y la utilización de las Tecnologías de la Información y Comunicaciones (TIC's en la cadena de suministro y su nivel de aplicación en la industria colombiana a través del análisis de algunos estudios existentes. Para esto se parte de conceptos generales, hasta llegar a las TIC's logísticas, las cuales varían según las características y necesidades de la cadena de suministro. A partir de dicha revisión se observó que estas tecnologías en su mayoría contribuyen a la reducción de costos y a mejorar el flujo de información entre los actores de la misma. Los principales obstáculos para la implementación de las TIC's en la cadena de suministro son los altos costos, la cultura de la organización y la inadecuada estructuración de los procesos. Con relación a su uso en Colombia, se identificó en los estudios analizados, un bajo grado de implementación en las pequeñas y medianas empresas (PYMES y un nivel medio en las grandes empresas y operadores logísticos.

  16. CARACTERIZACIÓN DE LA FASE UPSTREAM DE LA CADENA DE VALOR Y ABASTECIMIENTO DE LA AGROINDUSTRIA DE LA PALMA DE ACEITE EN COLOMBIA

    Directory of Open Access Journals (Sweden)

    Rafael García Cáceres

    2013-01-01

    Full Text Available Este artículo proporciona una descripción de la fase upstream de la cadena agroindustrial de la palma de aceite. El trabajo caracteriza la funcionalidad y relaciones de eslabones y escalones de la cadena de abastecimiento y de valor de la cadena en Colombia. El trabajo representa un esfuerzo pionero que busca contribuir a mejorar la eficiencia y eficacia del sector palmero colombiano.

  17. Canine specific ELISA for coagulation factor VII

    DEFF Research Database (Denmark)

    Knudsen, Tom; Kjelgaard-Hansen, Mads; Tranholm, Mikael; Wiinberg, Bo; Clausen, Jes T.; Hansen, Jens Jacob; Nichols, Timothy C.; Kjalke, Marianne; Jensen, Asger Lundorff; Kristensen, Annemarie Thuri

    2011-01-01

    Canine coagulation factor VII (FVII) deficiency can be hereditary or acquired and may cause life threatening bleeding episodes if untreated. FVII procoagulant activity can be measured by FVII activity (FVII:C), but assays for measurement of canine specific FVII antigen (FVII:Ag) have not been...... available to date. In this study, a canine specific ELISA for measurement of FVII:Ag in plasma was developed and validated. The FVII:Ag ELISA correctly diagnosed homozygous and heterozygous hereditary FVII deficiency. Together with activity based assays, such as FVII:C, the FVII:Ag ELISA should be valuable...... in the diagnosis of hereditary canine FVII deficiency....

  18. “ANÁLISIS DE LA CADENA AGROALIMENTARIA DE CONSERVAS DE TOMATE NATURAL EN LA PROVINCIA DE MATANZAS”

    Directory of Open Access Journals (Sweden)

    Neyfe Sablón Cossío

    2015-01-01

    Full Text Available El presente artículo tiene como objetivo principal, analizar la cadena agroalimentaria de conservas de tomate natural en la provincia de Matanzas. En la actualidad existen deficiencias en el abastecimiento de alimentos en las cadenas cubanas, se hace necesario mostrar una solución inmediata para elevar la producción y disminuir las brechas en la logística que une la producción primaria con las redes comercializadoras, para ofrecer los alimentos frescos o procesados a los consumidores. En este trabajo se presenta un estudio del procedimiento y posteriormente como se aplica en la red de conservas de tomate natural en la ciudad de Matanzas. En general, el enfoque en cadenas agroalimentarias impacta de forma positiva en la satisfacción de los clientes. Como aporte económico fundamental, la mejora de la disponibilidad de productos como indicador base del funcionamiento de una cadena de suministro, conlleva a la elevación de las ventas en la red de mercados. Elevar la satisfacción de las necesidades alimentarias de la población y facilitar el trabajo de apropiación que deben realizar los consumidores para adquirir alimentos frescos en el territorio, constituye el aporte social del análisis de las cadenas de suministro

  19. Establishment of swine interleukin-6 sandwich ELISA.

    Science.gov (United States)

    Nuntaprasert, A; Mori, Y; Tsukiyama-Kohara, K; Kai, C

    2005-03-01

    We established a sandwich enzyme-linked immunosorbent assay (ELISA) for swine interleukin-6 (SwIL-6), which was applied for detection of SwIL-6 in vitro and in vivo. Anti-SwIL-6 rabbit- and goat-polyclonal antibodies, and monoclonal antibody (mAb) were prepared, conforming that all of the antibodies were reactive with recombinant SwIL-6 by Western blotting and indirect ELISA. A sandwich ELISA was developed using the mAb as a capture antibody and biotinylated goat-polyclonal antibody as a detection antibody. The detection limit of the sandwich ELISA for rSwIL-6 was 49pg/ml and did not show cross-reactivity with swine IL-1b, IL-4, IL-8, IL-18, IL-12, and IFN-g. Using the ELISA, SwIL-6 was detected in culture medium of the monocytes stimulated with PHA-P and PMA, and the plasma or the bronchoalveolar lavage fluid (BALF) of pigs experimentally infected with Actinobacillus pleuropneumoniae or Mycoplasma hyopneumoniae. This ELISA for SwIL-6 may be useful for understanding the role of this cytokine in various swine diseases. PMID:15582688

  20. Field trial of brucellosis competitive ELISA

    International Nuclear Information System (INIS)

    2990 sera samples from cattle were tested for antibodies to Brucella abortus using 8 serological tests for. The tests used were Rose Bengal (RBT), Buffer Plate Agglutination Test (BPAT), Complement Fixation (CFT), 2 Indirect and 2 Competitive Enzyme Linked Immunosorbent Assays (ELISA). Bacteriological evaluation from milk was done also. All tests were compared with respect to diagnostic specificity in vaccinated herds which were considered to be Brucella-free. The diagnostic specificity of the Indirect and Competitive ELISA was greater than 99,8%. Estimates of relative sensitivity were obtained from infected herds. The diagnostic sensitivity of the Indirect ELISA was greater than 95,8% and for the Competitive ELISA between 98,8 and 100 %, the last value refers to the Competitive ELISA Prototype II (SLPS antigen/M84 Mab), which was found highly suitable to differentiate vaccinated from brucella-infected cattle. The use of C-ELISA II for monitoring bovine populations under an eradication programme is recommended. (author)

  1. Edades del basamento pre-varisco en Iberia: Herencia Icartiense, Grenville y Cadomiense en rocas del Complejo Olio de Sapo (NW de España). Estudio geocronológico mediante ablación láser

    OpenAIRE

    Fernández Suárez, Javier; Gutiérrez Alonso, Gabriel; Jenner, G. A.; Tubrett, M.

    1998-01-01

    [ES] Se han datado circones extraídos de rocas pertenecientes al complejo ígneo-vulcanosedimentario Olio de Sapo mediante la técnica de Ablación Laser ICPMS. Los resultados U-Pb en cristales individuales de circón indican la existencia de, al menos, tres eventos pre-Paleozoicos de formación de corteza en el NW de Iberia. Las edades más antiguas (ca 1,9-2 Ga) corresponden al evento Icartiense, que se ha identificado en otras áreas de la cadena Varisca de Europa occidental. El segundo evento (c...

  2. Publicidad expandida mediante realidad aumentada

    OpenAIRE

    Martí Parreño, José

    2011-01-01

    La realidad aumentada, aplicada al marketing, abre numerosas oportunidades para propiciar la decisión de compra del consumidor. Éste puede ver cómo un producto “cobra vida” mediante una pantalla en la que se superponen imágenes e información digital a la del entorno real que está viendo en ese mismo momento. En España, los consumidores ya han podido ver cómo empresas como El Corte Inglés o Doritos empleaban esta novedosa tecnología para promocionar sus productos. El autor plantea, por tanto, ...

  3. Las relaciones en la cadena de suministro no son tan peligrosas

    OpenAIRE

    Teresa Vallet-Bellmunt

    2010-01-01

    Este trabajo analiza cómo las relaciones en la cadena de suministro, a través de la estrategia de integración, pueden ser beneficiosas para sus miembros en términos de resultados logísticos, de marketing e incluso corporativos. Se estudia, a través de los “arcos de integración”, la influencia tanto de la amplitud como de la dirección de la integración (aguas arriba o abajo) en una muestra de 99 empresas pertenecientes a un distrito industrial. Se ofrece evidencia de que el arco...

  4. Indicadores logísticos en la cadena de suministro como apoyo al modelo scor

    Directory of Open Access Journals (Sweden)

    Abdul Zuluaga Mazo

    2015-01-01

    Full Text Available Este artículo tiene como principal objetivo revisar, analizar y proponer el uso de indicadores en los diferentes procesos logísticos de la cadena de suministro, los cuales cubren desde el aprovisionamiento pasando por el almacenamiento, la producción, el servicio al cliente, entre otros. Como resultado del desarrollo de un trabajo de investigación llamado « Estrategias logísticas para el abastecimiento de las pymes del sector confección del municipio de Itagüí». Se concluye que los indicadores planteados permiten medir el desempeño de los diferentes procesos logísticos en la cadena de suministro, lo cual, se convierte en la base para control del uso de los recursos, seguimiento al cumplimiento de objetivos e identificación de oportunidades de mejoramiento. La metodología que se utilizó para definir los indicadores fue a través del análisis de bibliografía especializada y la creación de unos indicadores propios a partir de modelos de referencia estándar de algunas empresas. Adicionalmente, los indicadores que se presentan sirven de apoyo a la medición del modelo de cadena de suministro SCOR, lo cual, se convierte en un valor agregado para el ámbito académico y empresarial.Palabras clave: Cadena de Suministro, nivel de desempeño, indicador, logística, KPI’s (Indicadores  de desempeño.Logisticals indicators in the supply chain as support to scor model.This paper has as its main objective of reviewing, analyzing and propose the use of indicators in different logistics processes  in the supply chain, which range from the supply, warehouse, production, customer service, among others. As a result of developing the document, supported by a previous research work called « Estrategias logísticas para el abastecimiento de las pymes del sector confección del municipio de Itagüí». We obtain indicators to measure the performance of the different logistics process in the supply chain, which becomes the basis to control the

  5. Fases de la cadena de suministro de las empresas avícolas

    OpenAIRE

    Bertila J. Aponte F.; Abraham J. González; Angel D. González P.

    2013-01-01

    En la cadena de suministro de las empresas avícolas se realiza una gran cantidad de procesos que originan una brecha de tiempo y espacio entre el insumo y el producto final, dificultando el adecuado análisis de sus elementos. Por esto, el objetivo del presente artículo es analizar las fases de la misma, específicamente en lo correspondiente a la producción de pollo. La investigación fue de tipo analítica, no experimental; con un diseño transeccional. La muestra estuvo conformada por 4 e...

  6. Metodología GLOCAL aplicada a la cadena de valor alimentaria.

    OpenAIRE

    Briz Escribano, Julian; Felipe Boente, Isabel de

    2013-01-01

    En la actualidad lo que más interesa es el abastecimiento y la seguridad alimentaria ? señalaba Julián Briz Escribano- uno de nuestros expositores invitados al Convite? Comercialización y Cadenas de Valor Alimentaria CVA como instrumento para el desarrollo socioeconómico y Agricultura Urbana?, organizado por el Instituto de Desarrollo Rural para Sudamérica IPDRS en colaboración del Posgrado en Ciencias del Desarrollo de la Universidad Mayor de San Andrés CIDES UMSA; y Agrónomos y Veterin...

  7. Fisiopatología articular y cadenas lesionales en el miembro inferior

    OpenAIRE

    Fernández Sánchez, Manuel

    2014-01-01

    Introducción: Una disfunción somática se caracteriza por una restricción de movilidad parcial, normalmente, de los elementos cinéticos. Corresponde a un ajuste defectuoso de la estructura que actúa como una causa primitiva o agravante de un desorden o enfermedad. Cualquier lesión de miembro inferior, y concretamente generada sobre el pie, ocasiona una serie de adaptaciones fisiopatológicas que se trasmiten en cadenas lesionales ascendentes provocando síntomas o signos cranealmente. ...

  8. Plan Estratégico Productivo Buenos Aires 2020. Sub-espacio costero. Cadena: turismo

    OpenAIRE

    Mantero, Juan Carlos; Benseny, Graciela; Castellucci, Daniela I.; Cruz, Gonzalo; González, María Graciela; Varisco, Cristina

    2012-01-01

    El turismo es una de las actividades base en la mayoría de los municipios que integran el sub-espacio costero. Como actividad productiva, pertenece al sector terciario y tiene un fuerte impacto en el PBG y en el empleo. Con un desarrollo que en algunos destinos comienza a fines del siglo XIX y continúa actualmente con el surgimiento de nuevas localidades, su historia ha marcado la identidad de varias comunidades, en especial, las ubicadas sobre el litoral marítimo. Como cadena productiva, el ...

  9. Gestion de la cadena de suministro para las Pymes exportadoras del cluster textil confeccion en Antioquia

    OpenAIRE

    Guisao Giraldo, Erica Yaneth

    2014-01-01

    Resumen: El presente trabajo de investigación estudia la gestión de la cadena de suministro para las pymes exportadoras del clúster textil confección en Antioquia, tema motivado por la participación del sector en el PIB del país y los indicadores de empleabilidad que reportan las empresas objeto de este estudio. La metodología utilizada fue cuantitativa con un enfoque analítico, método de muestreo y el tipo de muestra estratificada, definiendo el diseño de la muestra con los principios del m...

  10. Impacto de infraestructura de calidad en la cadena láctea

    OpenAIRE

    Claudia Santo; Elizabeth Ferreira

    2011-01-01

    Las acciones realizadas en forma sistémica por los diferentes actores de la infraestructura de la calidad a nivel nacional han potenciando la calidad a lo largo de toda la cadena productiva y logrado los efectos esperados de mejora de los productos lácteos en el país para su mejor introducción en el mercado internacional.En el sector lácteo uruguayo, los actores de la infraestructura de la calidad son el Ministerio de Ganadería, Agricultura y Pesca (MGAP), en su papel de reglamentador y certi...

  11. Trazabilidad en el primer eslabón de la cadena alimentaria

    OpenAIRE

    Roza, M.B. (María); Soldado, A.B. (Ana); Fernández, M. V.

    2009-01-01

    La falta de controles y la ausencia de mecanismos que permitieran seguir el rastro de los productos, tanto de forma individual como por lotes, fueron los responsables de que en su día se registraran diversas crisis alimentarias como la de "dioxinas", "vacas locas", etc. y se expandieran a lo largo de la cadena alimentaria. Esta situación fue minando la confianza de los consumidores en la capacidad de la industria y en las Administraciones Públicas para garantizar la inocuidad de los alimentos...

  12. Indicadores logísticos en la cadena de suministro como apoyo al modelo scor

    OpenAIRE

    Abdul Zuluaga Mazo; GÓMEZ MONTOYA, RODRIGO A.; Sergio A. Fernández Henao

    2015-01-01

    Este artículo tiene como principal objetivo revisar, analizar y proponer el uso de indicadores en los diferentes procesos logísticos de la cadena de suministro, los cuales cubren desde el aprovisionamiento pasando por el almacenamiento, la producción, el servicio al cliente, entre otros. Como resultado del desarrollo de un trabajo de investigación llamado « Estrategias logísticas para el abastecimiento de las pymes del sector confección del municipio de Itagüí». Se concluye que los indicadore...

  13. Cadena de restaurantes de comida cosmopolita y saludable a precio asequible

    OpenAIRE

    Álvarez García-Luján, Carlos

    2012-01-01

    El objeto de este trabajo es la presentación del proyecto de una “Cadena de restaurantes de comida cosmopolita y saludable a precio asequible” en la ciudad de Barcelona Se ofrecerán desayunos, comidas, meriendas, cenas e incluso “copas”, tanto en el propio local como en “take away”, abarcando así todo el abanico de demanda en alimentación fuera del hogar. Dicho proyecto basa su éxito en: • Las economías de escala y el poder negociador en las compras, basadas en un alto volumen ...

  14. An overview on ELISA techniques for FMD

    Directory of Open Access Journals (Sweden)

    Ding Yao-zhong

    2011-09-01

    Full Text Available Abstract Background FMD is one of the major causes of economic loss of cloven-hoofed animals in the world today. The assessment of dominant genotype/lineage and prevalent trends and confirmation the presence of infection or vaccination not only provides scientific basis and first-hand information for appropriate control measure but also for disease eradication and regaining FMD free status following an outbreak. Although different biological and serological approaches are still applied to study this disease, ELISA test based on the distinct format, antigen type and specific antibody reinforce its predominance in different research areas of FMD, and this may replace the traditional methods in the near future. This review gives comprehensive insight on ELISA currently available for typing, antigenic analysis, vaccination status differentiation and surveillance vaccine purity and content at all stages of manufacture in FMDV. Besides, some viewpoint about the recent advances and trends of ELISA reagent for FMD are described here. Methods More than 100 studies regarding ELISA method available for FMD diagnosis, antigenic analysis and monitor were thoroughly reviewed. We investigated previous sagacious results of these tests on their sensitivity, specificity. Results We found that in all ELISA formats for FMD, antibody-trapping and competitive ELISAs have high specificity and RT-PCR (oligoprobing ELISA has extra sensitivity. A panel of monoclonal antibodies to different sites or monoclonal antibody in combination of antiserum is the most suitable combination of antibodies in ELISA for FMD. Even though from its beginning, 3ABC is proven to be best performance in many studies, no single NSP can differentiate infected from vaccinated animals with complete confidence. Meanwhile, recombinant antigens and peptide derived from FMDV NPs, and NSPs have been developed for use as an alternative to the inactivated virus antigen for security. Conclusions There is

  15. Ventajas competitivas: Una propuesta paso a paso de búsqueda y aplicación de cadena de valor adaptada a empresas aéreas. Caso Patagonia- Argentina

    Directory of Open Access Journals (Sweden)

    María Alejandra Gazzera

    2005-05-01

    Full Text Available El desarrollo del proceso de búsqueda de ventajas competitivas de una empresa se puede plantear como un esfuerzo que se sigue en tres grandes líneas de actuación: la búsqueda centrada en la empresa, centrada en el entorno, y centrada en los competidores. Si bien la búsqueda de ventajas competitivas está estrechamente interrelacionada y vinculada entre las tres líneas, el presente trabajo se centrará en la primera de ellas, es decir al interior de la empresa, sin perder de vista la relación que tienen las empresas con su entorno o mercado, mediante la aplicación de la cadena de valor de Michael Porter (1985 adaptada a las empresas aéreas que operaban desde y hacia la ciudad de Neuquen, puerta de entrada a la Patagonia Argentina Es necesario destacar que no existen al presente, año 2005, estudios de este tipo en el sector mencionado en Argentina, pero en otros sectores productivos y aún en empresas aéreas de otros países, la cadena de valor ha demostrado su utilidad como tecnología de reflexión estratégica para generar valor en el cliente y para dirigir los esfuerzos de la empresa hacia la consolidación o creación de ventajas competitivas sostenibles en el tiempo.

  16. LAS CADENAS PRODUCTIVAS, ESTRATEGIA DE DESARROLLO EMPRESARIAL PARA EL MUNICIPIO DE TAMUÍN, SAN LUIS POTOSÍ

    OpenAIRE

    Ana Diana Betancourt Enríquez; Hugo Daniel Navarro Payta

    2013-01-01

    El presente artículo tiene como principal objetivo el análisis del esquema de cadena productiva, como estrategia para el desarrollo competitivo empresarial del municipio de Tamuín, S.L.P., aunado al caso del Grupo Gusi Sociedad de Producción Rural de Responsabilidad Limitada de esta localidad. En la investigación se presentan lineamientos teóricos que incluyen la conceptualización de la cadena productiva, clasificación de la misma, ventajas de integración del esquema, y rubros de lo local a l...

  17. Financiación estructurada en la cadena productiva palma, aceites, grasas vegetales y biocombustibles en Colombia

    OpenAIRE

    Quintero Jaramillo, Camilo Eduardo

    2015-01-01

    Los principales hitos en proyectos de inversión en la cadena productiva denominada “palma, aceites, grasas vegetales y biocombustibles” en Colombia y su financiación no se encuentran acompasados con una oferta apropiada, la estructura de financiación ofrecida por el mercado no identifica la existencia de la cadena productiva o esta no se revela como tal ante el sistema financiero -- Haciendo una revisión del portafolio de productos y servicios ofrecido en la actualidad por el sistema financie...

  18. Contratación y facturación electrónica en la cadena de valor

    OpenAIRE

    Afellat Oddi, Narjiss

    2011-01-01

    Las Tecnologías de la Información y de la Comunicación (TIC) están adquiriendo cada vez más importancia en el ámbito empresarial ya que permiten enlazar la cadena de valor de una empresa con las cadenas de valor de sus proveedores y clientes. En este sentido, las TIC adquieren un valor estratégico, transformando la manera en que se realizan las actividades de producción de valor y la naturaleza de los enlaces entre ellas. En este marco conceptual, la Contratación y la Facturación electrónicas...

  19. Evaluación del desempeño ambiental bajo enfoque de cadena de abastecimiento verde

    OpenAIRE

    William Ariel Sarache-Castro; Yasel José Costa-Salas; Jhully Paulin Martínez-Giraldo

    2015-01-01

    Entre los enfoques existentes de gestión ambiental, la gestión de cadenas de abastecimiento verde (Green Supply Chain Managemen t, GSCM), se ha convertido en uno de los más relevantes en los últimos años. El en foque GSCM aborda todos los procesos y actividad es que componen la cadena de abastecimiento en los eslabones de aprovi sionamiento, manufactura y entregas, incluyendo también el diseñ o, las compras, el marketing y la logística inve rsa. Aunque la revisión bibliográfica permiti ó iden...

  20. Cadena del frío para la conservación de las vacunas en los centros de atención primaria de un área de Madrid: mantenimiento y nivel de conocimientos

    Directory of Open Access Journals (Sweden)

    Ortega Molina Paloma

    2002-01-01

    Full Text Available Fundamento. Las vacunas son medicamentos termolábiles y para garantizar su inmunogenicidad y eficacia protectora, dentro de los programas de inmunización, es imprescindible mantener la cadena de frío. El elemento fundamental en esta cadena es el personal responsable de las vacunas, que debe conocer las características de estabilidad de cada preparado con el fin de evitar errores durante su manipulación. El objetivo de este trabajo fue conocer cómo se realiza el mantenimiento de la cadena del frío en equipos de atención primaria de un área sanitaria de la Comunidad Autónoma de Madrid, así como establecer el grado de información que poseen los responsables de las vacunas con respecto a la termoestabilidad de las mismas. Métodos. Se ha realizado un estudio transversal en 46 puntos de vacunación en atención primaria. La recogida de los datos se realizó mediante entrevista personal por un único investigador. Resultados. La tasa de participación fue del 93,5% (43/46. En todos los casos existía termómetro de máxima y mínima y registro mensual de la temperatura. Se observó una temperatura inadecuada en tres ocasiones (6,97%. El porcentaje de profesionales que conocía el efecto que la congelación producía sobre las vacunas fue muy diverso: 53.5%, 51.2%, 44.2% y 53.5% para difteria-tétanos-pertussis (DTP, hepatitis B (VHB, polio oral (VPO y rubéola-sarampión-paperas (RSP respectivamente. Y sólo el 32% conocía el test de agitación. Conclusión. La formación de los profesionales sobre el efecto que las altas temperaturas ocasionan en las vacunas era correcta, pero es necesario reforzar su formación sobre la inestabilidad que presentan los preparados adsorbidos cuando se someten a congelación.

  1. Las relaciones en la cadena de suministro no son tan peligrosas

    Directory of Open Access Journals (Sweden)

    Teresa Vallet-Bellmunt

    2010-01-01

    Full Text Available Este trabajo analiza cómo las relaciones en la cadena de suministro, a través de la estrategia de integración, pueden ser beneficiosas para sus miembros en términos de resultados logísticos, de marketing e incluso corporativos. Se estudia, a través de los "arcos de integración", la influencia tanto de la amplitud como de la dirección de la integración (aguas arriba o abajo en una muestra de 99 empresas pertenecientes a un distrito industrial. Se ofrece evidencia de que el arco más amplio es el que obtiene mejores resultados. Todo ello nos permite proponer un marco de investigación futura que ayude a profundizar en el conocimiento de esta relación, así como una serie de implicaciones de gestión para que los directivos de las empresas no vean las relaciones en la cadena de suministro como peligrosas.

  2. Fases de la cadena de suministro de las empresas avícolas

    Directory of Open Access Journals (Sweden)

    Bertila J. Aponte F.

    2013-01-01

    adecuado análisis de sus elementos. Por esto, el objetivo del presente artículo es analizar las fases de la misma, específicamente en lo correspondiente a la producción de pollo. La investigación fue de tipo analítica, no experimental; con un diseño transeccional. La muestra estuvo conformada por 4 empresas avícolas del estado Zulia, siendo los gerentes informantes clave en la investigación. Los resultados indican que la fase de aprovisionamiento de la cadena de suministro avícola posee 9 eslabones que aplican alianzas comerciales, tecnológicas y de producción para mantenerse integrados. La fase de procesamiento está compuesta por 2 eslabones que operan bajo una estrategia de producción continua. Finalmente, la fase de distribución posee 4 eslabones, que conforman canales de distribución de varios intermediarios que distribuyen los productos de forma intensiva. Se concluye que la industria avícola posee una cadena de suministro compleja, formada por 15 eslabones que se encargan de producir, procesar y distribuir diferentes productos a los consumidores en el momento, cantidad y condiciones deseadas.

  3. La investigación en gestión de la cadena de suministro a nivel mundial

    OpenAIRE

    Luz Mary Nova Caldas

    2010-01-01

    Este artículo analiza el estado del arte en relación con la investigación sobre gerencia de la cadena de suministro. Se hace particular énfasis en las variables estudiadas y los principales resultados obtenidos. Varias encuestas aplicadas alrededor del mundo son analizadas para identificar patrones en este tema.

  4. La investigación en gestión de la cadena de suministro a nivel mundial

    Directory of Open Access Journals (Sweden)

    Luz Mary Nova Caldas

    2010-07-01

    Full Text Available Este artículo analiza el estado del arte en relación con la investigación sobre gerencia de la cadena de suministro. Se hace particular énfasis en las variables estudiadas y los principales resultados obtenidos. Varias encuestas aplicadas alrededor del mundo son analizadas para identificar patrones en este tema.

  5. Acil coenzima-a sintetasas de cadena larga (acsls) como biomarcadores citoserológicos en enfermedades inflamatorias o autoinmunes

    OpenAIRE

    Matesanz, F; Fedetz, María; Ndagire, D.; Alcina, Antonio; Sabio, José Mario

    2010-01-01

    [ES] Uso de las Acil-Coenzima-A sintetasas de cadena larga (ACSL) como biomarcadores cito-serológicos de enfennedades inflamatorias o autoinmunes, y especialmente de lupus eritematoso sistémico, y método de obtención de datos útiles para el diagnóstico y seguimiento de dichas enfennedades.

  6. Hackeando los ciber-riesgos en la cadena de suministro: Para entender los controles correctos, hay que mirar el sistema

    DEFF Research Database (Denmark)

    Sepúlveda Estay, Daniel Alberto; Khan, Omera

    2016-01-01

    Si se consideran tanto la creciente complejidad de las redes de abastecimiento, como la consecuente exposición de estas redes a interrupciones inesperadas ocasionadas por ciber-ataques, se requiere una forma más amplia para entender los ciber-riesgos en las cadenas de suministro. En este artículo...

  7. Diseño e implementación del data warehouse de una cadena de tiendas de ropa

    OpenAIRE

    Sallent Sánchez, José Luis

    2016-01-01

    Una importante cadena de ropa solicita propuestas para crear un sistema de base de datos que actúe como data warehouse centralizado. La finalidad es poder tomar decisiones analizando el funcionamiento del negocio. A lo largo del documento se explican las etapas realizadas para entregar una solución acorde a las necesidades del cliente.

  8. DIAGNÓSTICO MOLECULAR DE Campylobacter EN LA CADENA AVÍCOLA DESTINADA PARA CONSUMO HUMANO EN COSTA RICA

    Directory of Open Access Journals (Sweden)

    Leana Zumbado-Guti\\u00E9rrez

    2014-01-01

    Full Text Available Diagnóstico molecular de Campylobacter en la cadena avícola destinada para consumo humano en Costa Rica. El objetivo de este estudio fue determinar la presencia y la frecuencia de Campylobacter jejuni y Campylobacter coli, en la cadena de producción avícola en Costa Rica. Se evaluaron granjas, planta de proceso y puntos de venta del Gran Área Metropolitana. Para la detección y aislamiento de Campylobacter sp. se analizaron 84 muestras provenientes de la cadena avícola (24 muestras de carne de pollo tomadas en punto de venta, 20 enjuagues de carcasa y 40 de ciegos tomados en planta de beneficio, obtenidas en noviembre de 2012, siguiendo el protocolo ISO 10272- 1:2006 modificado por el Departamento de Agricultura de Estados Unidos (USDA, por sus siglas en inglés. De las 84 muestras analizadas, 36 (42,8% resultaron positivas para C. jejuni, y una (1,2% para C. coli. La cantidad de muestras positivas según la Reacción en Cadena de Polimerasa (PCR, por tipo de muestra fue: para contenido cecal, 16 (n=40, para carcasas, 8 (n=20 y para punto de venta 12 (n=24. Dieciséis de las veinte granjas muestreadas fueron positivas. Hubo una alta frecuencia de Campylobacter en todos los puntos de la cadena muestreados, lo que podría estar asociado a modificaciones de parámetros relacionados con inocuidad en cada eslabón. El enfriamiento rápido y el uso de cloro en el agua en la planta de proceso, disminuyó la frecuencia de muestras positivas de Campylobacter spp.

  9. Análisis y optimización de una cadena de suministro con logística inversa e incertidumbre en la demanda

    OpenAIRE

    Pereda Pons, Julian

    2014-01-01

    Este proyecto tiene como objetivo desarrollar, optimizar y analizar una cadena de suministro basada en una arquitectura multiagente que permite simular el comportamiento de los diferentes procesos de la cadena ante la incertidumbre y variabilidad de la demanda del consumidor. Se utiliza un modelo de cadena de suministro presente en la literatura académica que introduce la logística inversa y analiza el impacto de la incertidumbre en el reciclaje para una posterior utilización en la producc...

  10. Elisa Studio showroom = Elisa Studio showroom / Jan Joonas Graps ; kommenteerinud Tiina Kuusisto

    Index Scriptorium Estoniae

    Graps, Jan Joonas, 1972-

    2015-01-01

    Elisa Studio showroom Helsingis Lasiplatsi, Mannerheimintie 22-24. Sisekujunduse autorid Jan Joonas Graps, Ken-Kristjan Ruut, Anne Määrmann (JanKen Wisespace). Lühidalt loovstuudiost JanKen Wisespace

  11. Planificación operativa del transporte en una cadena de suministro mediante un enfoque interactivo de programación lineal fuzzy multiobjetivo

    OpenAIRE

    Díaz-Madroñero Boluda, Francisco Manuel; Peidro Payá, David; Mula, Josefa

    2012-01-01

    [EN] In this paper, we propose a new fuzzy multi-objective linear programming model (FMOLP) for the supply chain operational transport planning problem that considers simultaneously the fuzziness in the aspiration levels and uncertainty in some critical parameters such as transport capacity levels. We also present an interactive solution methodology to convert this FMOLP model into an auxiliary crisp single-objective linear model and to find a preferred compromise solution in an interactive f...

  12. Internacionalización de las PYMES mediante las cadenas de valor : Cuál es el rol del financiamiento?

    OpenAIRE

    Avendaño, Rolando; Daude, Christian; Perea, José Ramón

    2013-01-01

    Más del 70 por ciento del producto interno bruto (PIB) en América Latina lo producen las grandes empresas, mientras que en las economías desarrolladas de la OCDE la participación de las grandes empresas en el PIB es de apenas el 40 por ciento. En materia de internacionalización, la situación de las PYMES latinoamericanas es incluso menos favorable, pues sus vínculos directos o indirectos con los mercados internacionales a través de las exportaciones son escasos. Estas débiles conexiones entre...

  13. Utilidad de sangre almacenada en papel de filtro para estudios serologicos por ELISA de inhibicion Use of filter paper strips on an ELISA inhibition test for serologic studies on dengue

    OpenAIRE

    Vázquez, S.; Fernández, R.; C. LLorente

    1991-01-01

    Para evaluar la utilidad del método de recolección de muestras de sangre en papel de filtro para la detección de anticuerpos anti-dengue mediante un ELISA de Inhibición recientemente desarrollado en nuestro laboratorio, se realizó una toma simultánea de muestras de sangre en papel de filtro y de suero, de donantes de Banco de Sangre. Ambas muestras fueron conservadas a -20ºC y probadas a los 15 dias, 3 y 6 meses respectivamente. A las muestras que resultaron positivos se les amplió el rango d...

  14. Cadenas agroalimentarias orgánicas en el sur de chile: tensiones que condicionan su puesta en valor

    OpenAIRE

    Sandra Ríos Núñez; Leonor Núñez Yáñez

    2016-01-01

    El modelo industrial de producción de alimentos ha incentivado la emergencia de cadenas agrarias alternativas. Estas suponen modelos de producción fuertemente vinculados al territorio y tienen la finalidad de transitar desde la producción industrial a la captura de valor por medio de sistemas de producción que garanticen la seguridad y soberanía alimentaria. Esta investigación analiza el funcionamiento de la agricultura orgánica en ...

  15. Construyendo Cadenas de Valor Incluyentes: una comparación de dos casos de biocomercio en Suramérica

    NARCIS (Netherlands)

    Muradian, R.; Verschoor, G.M.; Bolívar, E.; Ochoa, G.

    2012-01-01

    Este artículo analiza la configuración de redes de innovación en dos casos de cadenas de valor de biocomercio en Suramérica: producción de salsas de ajíes con frutas amazónicas en Colombia y producción de perfumes en Brasil a partir de aceites esenciales provenientes de Ecuador. Por medio de la inte

  16. La Cadena SER en Facebook: nuevas estrategias de interacción con la audiencia

    Directory of Open Access Journals (Sweden)

    Ramos del Cano, Fátima

    2013-06-01

    Full Text Available En español: El actual contexto periodístico ha consolidado a las redes sociales como nuevas y determinantes herramientas de comunicación e interacción con los usuarios. En este sentido, este artículo tiene por objeto analizar de qué manera la tradicional participación de la audiencia en el medio radiofónico se ha visto afectada por su implantación, centrando nuestro objeto de estudio en el perfil de Facebook (la red de mayor audiencia en España y una de las más significativas a nivel internacional de los programas informativos de la Cadena SER: "A Vivir que son dos días", "Hora 25", "Hoy por Hoy" y "La Ventana". Partiendo de la hipótesis de su infrautilización y aplicando el análisis de contenido, se ha estudiado la actividad de estos programas en sus cuentas de Facebook. Los resultados certifican cómo la emisora no potencia la interacción con los oyentes a través de sus redes sociales. La audiencia, sin embargo, sí que emplea estas plataformas para relacionarse entre sí. La llegada de este medio social, por tanto, ha favorecido y promocionado dinámicas de participación que, a diferencia de las tradicionales, no son impulsadas, ni canalizadas por el medio radiofónico sino por los propios oyentes. In english: The current news media context has consolidated social networks services as the new and decisive communication and interaction tools with users. In this regard, the purpose of this article is to analyze how the traditional audience participation has been affected by its implantation, focusing our inquiry on Facebook profile (being Facebook the network with the greatest audience in Spain and one of the most significant at the international level of Cadena Ser news programmes: "A Vivir que son dos días", "Hora 25", "Hoy por Hoy" and "La Ventana". Taking as the starting-point the hypothesis that it is being underutilized and applying the content analysis, it has been studied the activity of these programmes in regard to

  17. La cadena de suministro del sector metalúrgico: un análisis de sus principales eslabones (The Supply Chain in the metallurgical sector: an analysis of the main links)

    OpenAIRE

    Patricia Carbajo-de-Lera; Rafaela Alfalla-Luque; Carmen Medina-Lopez

    2011-01-01

    Los directivos actuales conciben que la competencia ya no es empresa frente a empresa, sino que desborda este plano generándose una competencia cadena de suministro frente a cadena de suministro. Por ello, la gestión de la cadena de suministro se convierte en un elemento clave para la supervivencia empresarial y la obtención de ventajas competitivas. El diseño de la cadena de suministro viene definido, en buena parte, por las características del sector al que pertenece. En consecuencia, es de...

  18. Modelo de benchmarking de la cadena de abastecimiento para pymes manufactureras.

    Directory of Open Access Journals (Sweden)

    Alfredo Beltrán Amador

    2002-01-01

    Full Text Available El modelo de benchmarking de la cadena de abastecimiento para Pymes manufactureras representa una guía para el mejoramiento de los procesos logísticos de una Pyme dedicada a la producción. En el presente artículo se muestra tanto el desarrollo del modelo como los principales resultados de su implementación en una muestra de empresas del Prodes (Programa de Desarrollo Sectorial de alimentos de Acopi, de la ciudad de Cali. Con base en los resultados de este estudio de aplicación demostrativa y de prueba, se establecen pautas para futuras investigaciones y aplicaciones del modelo en otros sectores. Este modelo ha sido desarrollado con base en los hallazgos del estudio titulado «Propuesta para el desarrollo de un sistema logístico integral para Pymes», realizado por los autores, como opción de grado para el título de Maestría en Administración, en convenio con la Universidad de Tulane.

  19. Cadenas Globales de Valor y Desarrollo: Perspectivas Críticas desde el Sur Global

    Directory of Open Access Journals (Sweden)

    Víctor Ramiro Fernández

    2015-06-01

    Full Text Available Este artículo realiza una interpretación teórica crítica de las cadenas globales de valor, entendidas como una relevante herramienta para el análisis de los procesos de globalización, no sólo a nivel académico sino a nivel institucional, a partir de la formulación de estrategias de desarrollo promovidas por gobiernos y organismos internacionales en el Sur Global. Teniendo en cuenta la evolución del concepto a partir de una perspectiva comparada con la teoría del sistema-mundo que le diera origen, se destacan las debilidades fundadas tanto en aspectos ligados al distanciamiento respecto de dicha teoría, como en los limitantes que surgen producto de la plataforma teórica que ambas comparten. Las restricciones para comprender la inalterada recreación de la estructura jerárquica y desigualadora del capitalismo, y los divergentes escenarios al interior del Sur Global, emergen como los limitantes teórico-empíricos más importantes. En este sentido, el trabajo concluye subrayando algunos desafíos orientados a superarlos.

  20. Mycotoxin determination using RIA and ELISA methods

    International Nuclear Information System (INIS)

    Experience is summed up of various authors with the determination of some mycotoxins (aflatoxin, ochratoxin A, T-2 toxin, rubratoxin, zearalenon, sterigmatocystine) using radioimmunoassay and enzyme immunoassay. For RIA purposes, tritium or sometimes iodine 125 is frequently used for labelling mycotoxins. Mycotoxins do not show immunogenic properties and must thus be conjugated with a high-molecular compound (serum albumin, polylysine) prior to immunozation. Factors are discussed making mycotoxin determination in foods difficult. Specificity of the obtained antisera is total between the individual mycotoxin groups while cross reactions are always recorded within the groups. RIA makes it possible to determine down to 200 pg (labelled with 3H) or 5 pg (labelled with 125I) of mycotoxins in a standard solution. In addition to high sensitivity and specificity, immunoassays of mycotoxins minimize the quantities of samples and solvents needed for extraction. Large series of samples can be processed using automatic analyzers. ELISA generally is more advantageous than RIA. (M.D.)

  1. eLISA and the Gravitational Universe

    Science.gov (United States)

    Danzmann, Karsten

    2015-08-01

    The last century has seen enormous progress in our understanding of the Universe. We know the life cycles of stars, the structure of galaxies, the remnants of the big bang, and have a general understanding of how the Universe evolved. We have come remarkably far using electromagnetic radiation as our tool for observing the Universe. However, gravity is the engine behind many of the processes in the Universe, and much of its action is dark. Opening a gravitational window on the Universe will let us go further than any alternative. Gravity has its own messenger: Gravitational waves, ripples in the fabric of spacetime. They travel essentially undisturbed and let us peer deep into the formation of the first seed black holes, exploring redshifts as large as z ~ 20, prior to the epoch of cosmic re-ionisation. Exquisite and unprecedented measurements of black hole masses and spins will make it possible to trace the history of black holes across all stages of galaxy evolution, and at the same time constrain any deviation from the Kerr metric of General Relativity. eLISA will be the first ever mission to study the entire Universe with gravitational waves. eLISA is an all-sky monitor and will offer a wide view of a dynamic cosmos using gravitational waves as new and unique messengers to unveil The Gravitational Universe. It provides the closest ever view of the early processes at TeV energies, has guaranteed sources in the form of verification binaries in the Milky Way, and can probe the entire Universe, from its smallest scales around singularities and black holes, all the way to cosmological dimensions.

  2. Las cadenas hoteleras en el mundo y evolución de su operación en México al inicio del siglo XXI

    Directory of Open Access Journals (Sweden)

    Alfonso de Jesús Jiménez Martínez

    2008-12-01

    Full Text Available Las cadenas hoteleras internacionales desempeñan un papel relevante en el sistema turístico internacional, y cada vez hay un mayor número de cuartos asociados a cadenas hoteleras en el mundo. Las más grandes, son empresas gigantescas que actúan a nivel global y con estrategias de escala planetaria. Las cadenas tienen particularidades específicas ligadas a la condición preponderante de los países desarrollados en la economía internacional, y su presencia mundial y regional está ligada a diversos factores que inciden en su funcionamiento. Actualmente, la dinámica de las grandes corporaciones se entiende más por la rentabilidad que promete a los accionistas que por la búsqueda de satisfacción del consumidor (el turista. En este texto se busca una aproximación a la identidad y la distribución regional de las cadenas en la oferta turística mundial y sus particularidades al inicio del siglo XXI. Se describe y analiza la evolución de esas cadenas en México de acuerdo con criterios de categoría y localización.

  3. Field trial of brucellosis competitive enzyme linked immunosorbent assay (ELISA)

    International Nuclear Information System (INIS)

    The purpose of this study was to compare the diagnostic performance characteristics of the competitive (C) ELISA for the diagnosis of bovine brucellosis and the differentiation of Brucella infected from Brucella vaccinated cattle to the indirect (I) ELISA and the conventional serological techniques used in Argentina. The diagnostic specificity and relative sensibility of ELISA was comparable to CFT and 2ME, which are the official complementary tests in Argentina. Besides, C-ELISA showed the best performancein analysing vaccinated animals. The application of the ELISA test is largely feasible because of its reproducibility and easy standardization. In addition, although CFT has a great performance, its application is cumbersom; the 2ME test lasted for 48 h and is toxic. Both the C-ELISA, using MAb M84, and the I-ELISA, using MAb M23, have been demonstrated to have the best performance. Owing to our field conditions (brucellosis prevalence and mandatory vaccination of female calves) the competitive ELISA should be applied as a complement of the official screening tests such as BP. These results suggest that the ELISA test would be very useful in contributing to the control and eradication programme of bovine brucellosis in Argentina

  4. ELISA versus Conventional Methods of Diagnosing Endemic Brucellosis

    Science.gov (United States)

    Mantur, Basappa; Parande, Aisha; Amarnath, Satish; Patil, Giridhar; Walvekar, Ravindra; Desai, Arun; Parande, Mahantesh; Shinde, Rupali; Chandrashekar, Masiyappa; Patil, Satish

    2010-01-01

    The diagnostic value of enzyme-linked immunosorbent assay (ELISA) was evaluated when blood specimens of 92 patients suspected of brucellosis underwent the ELISA (IgM and IgG), standard tube agglutination (SAT), and 2-mercaptoethanol (2-ME) tests and blood cultures; 38 sera from non-brucellosis patients and 34 sera from blood donors were also subjected to ELISA, SAT, and 2-ME tests. SAT was able to pinpoint only 23 (25%), whereas ELISA confirmed the etiology in 56 (60.9%; P < 0.001) patients with brucellosis, including 31 culture-confirmed cases. The sensitivity and specificity of ELISA were 100% and 71.31%, respectively. Because they were confirmed by ELISA, the diagnosis could never be excluded with SAT in 33 cases. ELISA has been found to be more sensitive in acute (28% higher sensitivity; P < 0.02) and chronic (55% higher sensitivity; P < 0.01) cases. For accurate diagnosis in suspected brucellosis cases detection, we recommend both ELISA IgM and IgG tests. ELISA IgG and 2-ME tests seem to be promising tools in judging prognosis. PMID:20682874

  5. Modelización de una cadena de abastecimiento (supply chain) para el sector textil-confección en el entorno colombiano

    OpenAIRE

    Ramírez Echeverri, Sergio Augusto

    2010-01-01

    Este trabajo modela la cadena de abastecimiento de la empresa Creaciones Nadar S.A, en la ciudad de Medellín y se utiliza la dinámica de sistemas. La empresa se dedica a la confección, distribución y comercialización de los productos de la marca Speedo® en todo el país y para el exterior. Se realizan entrevistas y encuesta con expertos en la cadena textil confección. Con la información se hace un modelo de la cadena de suministro, con dinámica de sistemas, y con la utilización del software It...

  6. SISTEMAS DE IDENTIFICACIÓN POR RADIOFRECUENCIA, CÓDIGO DE BARRAS Y SU RELACIÓN CON LA GESTIÓN DE LA CADENA DE SUMINISTRO

    Directory of Open Access Journals (Sweden)

    ALEXANDER CORREA ESPINAL

    2010-01-01

    Full Text Available El articulo muestra, a través del análisis de libros, artículos científicos y algunos estudios existentes, el estado del arte y la relación entre los sistemas e identificación (código de barras y radiofrecuencia y la gestión de la cadena de suministro, en aplicaciones significativas a nivel mundial y de Colombia. Para ello, se compilan conceptos generales de los sistemas de identificación presentando sus definiciones, componentes de hardware/software, aplicaciones en la cadena de suministro y el uso que grandes, medianas y pequeñas empresas le otorgan. El estudio pone en evidencia la importancia que estas tecnologías tienen para mejorar el desempeño y la trazabilidad, simplificar operaciones, reducir costos y agilizar los flujos de información en la cadena de suministro.

  7. Lean production y gestión de la cadena de suministro en la industria aeronáutica

    OpenAIRE

    Moyano Fuentes, José; Martínez Jurado, Pedro José

    2011-01-01

    En este trabajo se evalúa el estado actual de la investigación sobre Lean Production y Gestión de la Cadena de Suministro en la industria aeronáutica con dos objetivos básicos: 1) identificar el conjunto de líneas de investigación desarrolladas, aportando un criterio de clasificación de la literatura, 2) discutir sobre la evidencia empírica, intentando proporcionar orientaciones para la investigación futura. Para el logro de estos objetivos se ha empleado una metodología de investigación cons...

  8. Los españoles y la revolución comercial mexicana: las cadenas de supermercados, 1921-2011

    OpenAIRE

    Javier Moreno Lázaro

    2012-01-01

    En este artículo enfatizo el papel desempeñado por los españoles en la creación de las grandes cadenas de supermercados en México. La cercanía a Estados Unidos y la aportación española resultaron decisivas en su engrandecimiento. Esta historia, al tiempo, revela la importancia del papel desempeñado por las minorías nacionales en la formación de la gran empresa en América Latina. Al tiempo revela un aspecto desconocido hasta la fecha: la fortaleza de estas firmas comerciales, su capacidad de a...

  9. Modelamiento, con dinámica de sistemas, de una cadena de distribución de alimentos refrigerados

    OpenAIRE

    Montoya Moreno, Carlos Andrés

    2011-01-01

    En el capítulo 2, se presenta los conceptos de control y medición, como procesos que permiten lograr una buena gestión y que constituyen una importante base en la construcción de indicadores; posteriormente se desarrollara los conceptos principales de indicadores de gestión, indicadores de gestión logística e indicadores en cadenas de distribución; luego se describe un sistema de indicadores de gestión aplicado a la microempresa Lácteos y Cárnicos “MERCARDY”, finalmente se explica el funciona...

  10. Caracterización de la cadena de suministros de la miel en el estado de Tabasco.

    OpenAIRE

    Pascual González, Josué

    2012-01-01

    El presente trabajo presenta el resultado de la Caracterización de la Cadena de Suministros de la Miel (CSM) en el Estado de Tabasco y la Evaluación de Sistemas de Trazabilidad para la Miel. La CSM está integrada por una serie relaciones ascendentes y descendentes entre unidades de producción, para una fácil compresión en este trabajo, a las unidades de producción se les ha denominado actores de CSM. La caracterización de la CSM en el estado de Tabasco se realizó entre proveedores de mater...

  11. Modelo estocástico para planificar cadenas de suministro con productos de ciclos de vida cortos

    Directory of Open Access Journals (Sweden)

    Jairo Coronado-Hernandez

    2010-07-01

    Full Text Available En este trabajo se presenta un modelo de optimización estocástica para la planificación de cadenas de suministros para productos con ciclo de vida corto, a través de la decisión de reservar la capacidad a contratar en los recursos de los proveedores antes de tener certeza del comportamiento de la demanda. Se consideran recursos alternativos, múltiples productos con lista de materiales complejas, demanda distribuida a lo largo de periodos consecutivos, ciclos de vida cortos, lead time largos y altos niveles de incertidumbre representados en forma de escenarios.

  12. Reflexiones sobre la propuesta de reforma constitucional en Colombia para la introducción de la cadena perpetua:

    Directory of Open Access Journals (Sweden)

    Lina Mariola Díaz Cortés

    2009-06-01

    Full Text Available El artículo analiza los diferentes proyectos de ley que buscan dar respuesta a episodios esporádicos de violencia sexual contra menores. De esta forma se estudia la política criminal “irracional” que se ha propuesto en Colombia para los delitos sexuales contra menores, es decir, en concreto, las propuestas absurdas e injustificadas de implementar instrumentos como la cadena perpetua, que desconocen principios básicos contenidos dentro de nuestra Carta de derechos y que han sido acogidos desde antaño por penalistas que defienden la utilización racional de los instrumentos punitivos del Estado.

  13. Modelo estocástico para planificar cadenas de suministro con productos de ciclos de vida cortos

    OpenAIRE

    Jairo Coronado-Hernandez; Jose P. Garcia-Sabater

    2010-01-01

    En este trabajo se presenta un modelo de optimización estocástica para la planificación de cadenas de suministros para productos con ciclo de vida corto, a través de la decisión de reservar la capacidad a contratar en los recursos de los proveedores antes de tener certeza del comportamiento de la demanda. Se consideran recursos alternativos, múltiples productos con lista de materiales complejas, demanda distribuida a lo largo de periodos consecutivos, ciclos de vida cortos, lead time largos y...

  14. Desarrollo de un modelo de gestión del conocimiento en la cadena de suministro de la industria agroalimentaria

    OpenAIRE

    Martínez Soto, Moisés

    2011-01-01

    La industria agroalimentaria (IAA) tiene una importancia estratégica para la gran mayoría de las naciones. En el siglo XXI la competitividad y sostenibilidad de las organizaciones se basa en los activos de conocimiento y su gestión. En tal sentido, la gestión del conocimiento (GC) representa una estrategia que contribuye a mejorar el rendimiento y el desempeño de las cadenas de suministro de la IAA. Sin embargo, la cuantificación y análisis de esta contribución sigue siendo objeto de estudio ...

  15. Manejo estratégico de la calidad ambiental en las cadenas agroalimentarias. Aplicaciones a la arveja china guatemalteca

    OpenAIRE

    Jiménez Porras, Gerardo; Pelupessy, Wim

    2006-01-01

    Desde la perspectiva de las cadenas globales de mercancías, considerando la secuencia de actividades insumo-producto (principalmente producción primaria, procesamiento, comercialización y uso final), este artículo analiza las interacciones (horizontales) entre productores agrícolas como un juego del tipo dilema del prisionero. El resultado esperado es un equilibrio Nash en el cual los productores se quedan atados al uso de tecnologías no sostenibles. Sin embargo, la consideración de los movim...

  16. Las cadenas de franquicias en España: Estrategias empresariales y organización espacial

    OpenAIRE

    Agustín Gámir de Orueta; Ricardo Méndez Gutiérrez del Valle

    1998-01-01

    La expansión de las cadenas de franquicias en españa se ha convertido en una de las innovaciones que contribuye a transformas con rapidez, tanto la estructura del sector de distribución, como la propia fisonomía de los espacios de consumo en la ciudad. El estudio realizado propone una interpretación teórica sobre el significado de estas redes empresariales, que destaca tanto sus potencialidades como algunos de los costes y riesgos asociados, para centrar luego su atención en la evolución reci...

  17. Más por menos: El trabajo precario de las mujeres en las cadenas de producción globalizadas

    OpenAIRE

    Raworth, Kate; Harvey, Claire

    2004-01-01

    La globalización ha introducido a millones de mujeres de los países en desarrollo en el mundo del trabajo. Trabajan en las cadenas globales de producción de las empresas multinacionales, produciendo mercancías que impulsan el crecimiento de las exportaciones: recogen y envasan fruta, cosen prendas de vestir, cortan flores y montan juguetes. Estos trabajos podrían aportar a las mujeres los ingresos, la seguridad y el apoyo que necesitan para salir de la pobreza junto con sus familias. Por el ...

  18. Construyendo Cadenas de Valor Incluyentes: una comparación de dos casos de biocomercio en Suramérica

    OpenAIRE

    Muradian, R.; Verschoor, G.M.; Bolívar, E.; Ochoa, G.

    2012-01-01

    Este artículo analiza la configuración de redes de innovación en dos casos de cadenas de valor de biocomercio en Suramérica: producción de salsas de ajíes con frutas amazónicas en Colombia y producción de perfumes en Brasil a partir de aceites esenciales provenientes de Ecuador. Por medio de la integración de dos perspectivas teóricas, los autores resaltan tanto los aspectos económicos como socioculturales que influencian la configuración de estas redes. Esto permite considerar las tensiones,...

  19. Agricultural production - Phase 2. Indonesia. ELISA for epidemiology of brucellosis

    International Nuclear Information System (INIS)

    This document is a travel report of a three-week mission (from October 13 to November 1, 1991) to Indonesia within the framework of ''The implementation of ELISA technology for the sero-diagnosis of important livestock diseases''. The mission evaluated the implementation of ELISA technology at the Regional Laboratories and its role in the surveillance and control of bovine brucellosis

  20. Relación entre los niveles de flexibilidad de la cadena muscular posterior y lesiones músculo tendinosas en jugadores de rugby

    OpenAIRE

    Alessio, Germán

    2015-01-01

    La práctica de rugby produce posturas hipertónicas que traen como consecuencia un acortamiento de los músculos de la cadena posterior, lo que lleva aparejado una reducción de la flexibilidad. Objetivo: Establecer la relación entre los niveles de flexibilidad de la cadena muscular posterior con la cantidad de lesiones músculo-tendinosas en jugadores de rugby. Material y Métodos: La investigación es no experimental, transversal y descriptiva. Se evalúo la flexibilidad a través de un cajón...

  1. El Efecto Bullwhip en las Cadenas de Suministro: Una Aproximación Basada en Técnicas de Inteligencia Artificial

    OpenAIRE

    Ponte Blanco, Borja

    2013-01-01

    Varias son las circunstancias que, en las dos últimas décadas, han concedido a la gestión de la cadena de suministro un papel estratégico en la búsqueda de ventajas competitivas. Así, tras una exhaustiva revisión bibliográfica sobre la materia abordada, este documento aplica la metodología multiagente para optimizar dicha gestión. Para ello, representa la cadena de suministro como un Sistema Global Multiagente, compuesto a su vez por cuatro Subsistemas Multiagente, que replican el comportamie...

  2. Seguridad en la cadena de suministro basada en la norma iso 28001 para el sector carbón, como estrategia para su competitividad

    OpenAIRE

    CORREA ESPINAL, ALEXANDER; GÓMEZ MONTOYA, RODRIGO ANDRÉS

    2011-01-01

    El presente artículo busca revisar y analizar el aporte e impacto de la cadena de suministro y su seguridad, basándose en la adopción de la norma internacional ISO 28001:2007, como estrategia para mejorar la competitividad y vinculación a mercados internacionales del carbón colombiano. Para alcanzar este objetivo, se revisan libros, informes y artículos, que cubren desde la descripción del sector minero en Colombia, pasando por conceptos de cadena de suministro y su estructura en el sector...

  3. PROPUESTA DE UN MODELO DE INTEGRACIÓN PARA LA GESTIÓN DE LA CADENA DE SUMINISTRO EN EL SECTOR DE LA CONSTRUCCIÓN

    OpenAIRE

    CARBONELL UREÑA, JUAN FRANCISCO

    2012-01-01

    Hoy en día, y en nuestro entorno, el sector de la construcción está experimentando grandes cambios en todos los aspectos relacionados con su negocio: el mercado, las relaciones entre competidores, los mecanismos de financiación, los recursos de mano de obra, etc. es por ello que en esta Tesis se pretende analizar modelos existentes de integración de la Cadena de Suministro que recojan una manera de enfocar los problemas a los que se tiene que enfrentar este sector y las cadenas de producción ...

  4. SISTEMAS DE IDENTIFICACIÓN POR RADIOFRECUENCIA, CÓDIGO DE BARRAS Y SU RELACIÓN CON LA GESTIÓN DE LA CADENA DE SUMINISTRO

    OpenAIRE

    ALEXANDER CORREA ESPINAL; CARLOS ESTEBAN ÁLVAREZ LÓPEZ; RODRIGO ANDRÉS GÓMEZ MONTOYA

    2010-01-01

    El articulo muestra, a través del análisis de libros, artículos científicos y algunos estudios existentes, el estado del arte y la relación entre los sistemas e identificación (código de barras y radiofrecuencia) y la gestión de la cadena de suministro, en aplicaciones significativas a nivel mundial y de Colombia. Para ello, se compilan conceptos generales de los sistemas de identificación presentando sus definiciones, componentes de hardware/software, aplicaciones en la cadena de suministro ...

  5. Sobre la geología de la región de Archidona y la pertenencia de la Alta Cadena al Subbético

    OpenAIRE

    Pineda Velasco, Antonio

    1987-01-01

    [ES] Este trabajo propone una estructura de conjunto para la región de Archidona, basada en nuevas interpretaciones paleogeográficas y tectónicas para el Complejo de la Alta Cadena y el Trias de Antequera. Las conclusiones y consecuencias obtenidas simplifican la paleogeografía y la estructura de determinadas zonas de la Cordillera e implican las relaciones entre las zonas Externas e Internas. El Complejo de la Alta Cadena representa un dominio paleogeográfico entre el Subbético medio central...

  6. CONSIDERACIONES SOBRE LA PLANIFICACIÓN DE PRODUCTOS ALIMENTICIOS EN UNA CADENA DE SUMINISTRO COMERCIAL / CONSIDERATIONS ON FOODSTUFF PLANNING IN A COMMERCIAL SUPPLY CHAIN

    OpenAIRE

    Neyfe Sablón-Cossío; Alberto Medina-León; José Antonio Acevedo-Suárez; Ana Julia Acevedo-Urquiaga; Teresita López-Joy

    2013-01-01

    En la actualidad, la necesidad de pasar a la gestión de cadenas de suministro en la economía cubana se refleja a través de diversos síntomas que expresan la falta de coordinación entre las diversas redes de empresas. El presente trabajo muestra la aplicación de este enfoque a la cadena de suministro del puré de tomate, desde la base productiva nacional que abastece la industria, las importaciones de conservas y su abastecimiento a la red de mercados. Se realiza un análisis de la planificación...

  7. Análisis y evaluación de la cadena alimentaria de productos ecológicos para el fomento del desarrollo sostenible en zonas productoras

    OpenAIRE

    Briz de Felipe, Teresa

    2005-01-01

    La presente Tesis doctoral trata de ofrecer una visión global del sector de productos ecológicos desde el productor al consumidor, analizando su repercusión en el desarrollo de áreas marginales. Se ha tratado de englobar toda la cadena de valor del producto, no centrándose únicamente en aspectos parciales o en algún eslabón de la misma. Para ello, se realizaron encuestas personales o telefónicas a los agentes de los tres eslabones de la cadena alimentaria: producción, distribución y consumo, ...

  8. Caracterización de la cadena de abastecimiento en las pymes de la comunicación gráfica impresa en Colombia

    OpenAIRE

    2012-01-01

    La monografía “Caracterización de la cadena de abastecimiento en las pymes de la comunicación gráfica impresa en Colombia” es una investigación que identifica los componentes y la relación existente con las pymes (pequeñas y medianas empresas) en la cadena de abastecimiento de la comunicación gráfica en Colombia. El objetivo planteado para esta monografía es realizar una propuesta de desarrollo de proveedores en las pymes de la industria de la comunicación gráfica impresa en Colombia, tenie...

  9. Conocimiento y aprendizaje en las grandes cadenas hoteleras españolas en Iberoamérica. Internet como herramienta de aprendizaje organizativo

    OpenAIRE

    Rodríguez Antón, José Miguel; Alonso Almeida, Mar; Rubio Andrada, Luis; Esteban Alberdi, Cristina

    2008-01-01

    El aprendizaje y la dirección del conocimiento organizativo se han convertido en sendos elementos clave para la dirección de las cadenas hoteleras. El hecho de que múltiples tipos de clientes se alojen en sus hoteles dificulta un trato homogéneo y estandarizado que sea capaz de atender las preferencias específicas de cada cliente haciéndole sentir un huésped especial. Por este motivo, va a ser necesario conjugar una política general para toda la cadena con un trato personalizad...

  10. Conocimiento y aprendizaje en las grandes cadenas hoteleras españolas en Iberoamérica: internet como herramienta de aprendizaje organizativo

    OpenAIRE

    2008-01-01

    El aprendizaje y la dirección del conocimiento organizativo se han convertido en sendos elementos clave para la dirección de las cadenas hoteleras. El hecho de que múltiples tipos de clientes se alojen en sus hoteles dificulta un trato homogéneo y estandarizado que sea capaz de atender las preferencias específicas de cada cliente haciéndole sentir un huésped especial. Por este motivo, va a ser necesario conjugar una política general para toda la cadena con un trato personalizad...

  11. Comparison of three ELISA tests for seroepidemiology of bovine fascioliosis.

    Science.gov (United States)

    Ibarra, F; Montenegro, N; Vera, Y; Boulard, C; Quiroz, H; Flores, J; Ochoa, P

    1998-06-30

    The aim of the present study was to compare the sensitivity, specificity and usefulness of the DIG-ELISA, DOT-ELISA and Indirect ELISA tests for determining the seroprevalence of fasciolosis in cattle under tropical conditions in Mexico. To standardize the tests, positive and negative sera to F. hepatica from 88 Holstein Freisian adult cows located in an enzootic area of fascioliosis and 88 crossbred adult cattle from a fluke-free area were used. For the epidemiological study, 85 crossbred cattle between 1 to 7 years of age were used. Animals were bled every two months, from March 1995 to September 1996 and the sera obtained were stored at -70 degrees C, until used. Indirect ELISA showed a sensitivity of 96.5% and a specificity of 98.8%, DIG-ELISA 97.5% and 80.0% and DOT-ELISA 93.1% and 95.4%, respectively. During 1995, Indirect ELISA yielded the highest levels of IgG anti-F. hepatica antibodies. However, in 1996, after animal treatment with triclabendazole, DIG-ELISA tended to show higher percentages of antibody-positive animals, but it was not significantly different (p>0.05) from the other tests. Comparisons made in parallel to the faecal sedimentation test demonstrated that all serological tests detected higher percentages of positive animals. Only one serum out of ten (10%) of Paramphistomum spp. cross-reacted with the DOT-ELISA test, but no cross-reaction was observed with sera from animals with other parasites. All ELISA tests were highly sensitive and specific; they may be recommended for use in seroepidemiological surveys for F. hepatica. PMID:9763313

  12. Evaluación del desempeño ambiental bajo enfoque de cadena de abastecimiento verde

    Directory of Open Access Journals (Sweden)

    William Ariel Sarache-Castro

    2015-01-01

    Full Text Available Entre los enfoques existentes de gestión ambiental, la gestión de cadenas de abastecimiento verde (Green Supply Chain Managemen t, GSCM, se ha convertido en uno de los más relevantes en los últimos años. El en foque GSCM aborda todos los procesos y actividad es que componen la cadena de abastecimiento en los eslabones de aprovi sionamiento, manufactura y entregas, incluyendo también el diseñ o, las compras, el marketing y la logística inve rsa. Aunque la revisión bibliográfica permiti ó identificar la existencia de una gran v ariedad de indicadores de desempeño ambiental que se enmarcan en el enfoque PresiónEstadoRes puesta, no se encontraron indicadores aplica dos bajo un enfoque GSCM. Por tanto, el presente ar tículo expone una metodología para la cons trucción de un indicador de corte multicrit erio que permite evaluar el desempeño ambiental desde el enfoque GSCM, con base en 8 procesos y 32 actividades detectadas en la revisión de literatura. El indicador fue aplicado en un grupo de 14 empresas de la región cafetera de Colombia. Los resultados indican un d esempeño heterogéneo entre las compañías analizadas y un rezago importante en algunos procesos.

  13. LEAN PRODUCTION Y GESTIÓN DE LA CADENA DE SUMINISTRO EN LA INDUSTRIA AERONÁUTICA

    Directory of Open Access Journals (Sweden)

    Moyano Fuentes, José

    2011-01-01

    Full Text Available En este trabajo se evalúa el estado actual de la investigación sobre Lean Production y Gestión de la Cadena de Suministro en la industria aeronáutica con dos objetivos básicos: 1 identificar el conjunto de líneas de investigación desarrolladas, aportando un criterio de clasificación de la literatura, 2 discutir sobre la evidencia empírica, intentando proporcionar orientaciones para la investigación futura. Para el logro de estos objetivos se ha empleado una metodología de investigación consistente en la identificación y revisión de la literatura. El análisis sistemático posterior de ésta ha sido de naturaleza cualitativa. Este análisis ha permitido identificar tres líneas de investigación en torno a Lean Production y otras tres relacionadas con la Gestión de la Cadena de Suministro. Por último, se identifican nuevas oportunidades y retos a los que se enfrenta la investigación en un futuro.

  14. Plasmonic ELISA for the ultrasensitive detection of Treponema pallidum.

    Science.gov (United States)

    Nie, Xin-Min; Huang, Rong; Dong, Cai-Xia; Tang, Li-Juan; Gui, Rong; Jiang, Jian-Hui

    2014-08-15

    In this report, we have developed a plasmonic ELISA strategy for the detection of syphilis. Plasmonic ELISA is an enzyme-linked immunoassay combined with enzyme-mediated surface plasmon resonance (SPR) of gold nanoparticles (AuNPs). Immune response of the Treponema pallidum (T. pallidum) antibodies triggers the acetylcholinesterase-catalyzed hydrolysis of acetylthiocholine to produce abundant thiocholine. The positive charged thiol, in turn, alters the surface charge distribution the AuNPs and leads to the agglomeration of the AuNPs. The induced strong localized SPR effect of the agglomerate AuNPs can, thus, allow the quantitative assay of T. pallidum antibodies due to the remarkable color and absorption spectral response changes of the reaction system. The plasmonic ELISA exhibited a quasilinear response to the logarithmic T. pallidum antibody concentrations in the range of 1pg/mL-10ng/mL with a detection limit of 0.98pg/mL. Such a low detection limit was 1000-fold improvements in sensitivity over a conventional ELISA. The results of plasmonic ELISA in syphilis assays of serum specimens from 60 patients agreed with those obtained using a conventional ELISA method. The plasmonic ELISA has characteristics (analyte specific, cost-effective, ease of automatic, low limit of detection) that provide potential for diagnosis and therapeutic monitoring of syphilis. PMID:24662060

  15. Relaciones de asociatividad y cooperación de las empresas familiares medianas en la cadena productiva metalmecánica en la ciudad de Bogotá

    OpenAIRE

    2011-01-01

    En esta tesina se describe un marco teórico sobre las relaciones de asociatividad y cooperación entre las empresas para el desarrollo de sus ventajas competitivas, identificando los factores que las determinan, tomando las experiencias y modelos de cluster, cadenas productivas, redes y distritos industriales. Partiendo de las condiciones y necesidades de competitividad de las empresas familiares

  16. Aplicación del benchmarking en la gestión de la cadena de aprovisionamiento sanitaria: efectos sobre el coste y la calidad de las compras

    Directory of Open Access Journals (Sweden)

    David Naranjo-Gil

    2015-03-01

    Conclusión: Con un proceso de benchmarking, los centros sanitarios pueden ahorrar gastos y realizar una gestión más eficiente de la cadena de aprovisionamiento. El benchmarking facilita que los centros sanitarios adquieran productos a precios más reducidos y de mayor calidad.

  17. Análisis de la Cadena de Suministro y su relación con la Logística: caso del Centro Michelin Valladolid

    OpenAIRE

    Villafañe Alonso, Pablo

    2014-01-01

    El Alcance del Proyecto es el estudio y análisis general de los conceptos Cadena de Suministro y Logística y la aplicación en la empresa Michelin y en su Centro de Valladolid. Además, se proporcionan una serie de conclusiones y mejoras aplicables para la empresa.

  18. Modelo de diseño de nodos de integración en las cadenas de suministro/Design model of integration nodes in the supply chains

    Directory of Open Access Journals (Sweden)

    Yinef Pardillo-Baez

    2013-01-01

    Full Text Available La débil integración entre empresas de las cadenas de suministro, el hecho de que las entidadesno tienen un procedimiento para identificar los parámetros e indicadores de coordinación entreprocesos, la ineficiente gestión integrada de recursos y capacidades y el inadecuado uso de loscontratos como herramienta de coordinación entre los actores de la cadena de suministro; sonelementos que caracterizan la práctica empresarial de las cadenas de suministro cubanas. Elsiguiente trabajo plantea las bases del Modelo de Diseño de los Nodos de Integración en lascadenas de suministro, y sus aplicaciones. El Modelo es una herramienta de diseño, a la vez queapoya la toma de decisiones en los procesos. Se demuestra que al aplicar el modelo y suprocedimiento, la cadena obtiene mejores resultados e incrementa su integración. Comoconclusión fundamental se obtiene que el Modelo es aplicable y brinda resultados positivos para lapráctica empresarial cubana.

  19. Acil Coenzima-A sintetasas de cadena larga (ACSLs) como biomarcadores cito-serológicos en enfermedades inflamatorias o autoinmunes

    OpenAIRE

    Matesanz, F; Fedetz, María; Ndagire, D.; Alcina, Antonio; Sabio, José Mario

    2010-01-01

    Uso de las Acil-Coenzima-A sintetasas de cadena larga (ACSL) como biomarcadores cito-serológicos de enfermedades inflamatorias o autoinmunes, y especialmente de lupus eritematoso sistémico, y método de obtención de datos útiles para el diagnóstico y seguimiento de dichas enfermedades. Venta

  20. Agenda prospectiva de investigación y desarrollo tecnológico para la cadena productiva de Fique en Colombia.

    OpenAIRE

    Castellanos Domínguez, Óscar Fernando; Torres Piñeros, Luz Marina; Cateline Rojas, July

    2009-01-01

    En este libro se presenta los resultados de un interesante enfoque conceptual y metodológico para abordar el direccionamiento estratégico de sectores productivos con una visión prospectiva que se ha aplicado desde el año 2006 en 20 cadenas productivas.

  1. Comparison of dot-ELISA and standard ELISA for detection of Neisseria meningitidis outer membrane complex-specific antibodies

    Directory of Open Access Journals (Sweden)

    Elza FT Belo

    2010-02-01

    Full Text Available Dot-ELISA using the outer membrane complex antigens of Neisseria meningitidis as a target was standardized for rapid detection of meningococcal-specific antibodies in human serum. We investigated the level of meningococcal-specific IgG, IgA, and IgM in serum using dot-ELISA with outer membrane antigens prepared from Neisseria meningitidis serotype B:4.19:P1.15,3,7,9 (a strain isolated from a Brazilian epidemic. The dot-ELISA is based on the same principles as the standard ELISA and is useful for detection of anti-N. meningitidis B antibodies in serum of patients with meningococcal infections. For the assay, outer membrane complexes (OMCs were absorbed by nitrocellulose membrane and blocked with a 5% skim milk solution. Serum samples were drawn upon hospital admission and during convalescence from patients with meningococcal septicemia, and single samples were drawn from uninfected controls. We retrospectively examined a total of 57 serum samples: 35 from patients infected with N. meningitidis B, 12 from patients infected with Haemophilus influenzae b, and 10 from health individuals. When performed at room temperature, dot-ELISA took approximately four hours to perform, and the optimum antigen concentration was 0.42 µg per dot. The specificity of IgG, IgM, and IgA demonstrates that dot-ELISA using OMCs from N. meningitidis B as a target is suitable for serologic verification of clinically suspected meningococcal disease in patients and for titer determination of antibodies produced during different phases of natural infection. Furthermore, the sensitivity of dot-ELISA was comparable to that of standard ELISA. Overall, dot-ELISA is simple to perform, rapid, and low cost. Further validation of the test as a screening tool is required.

  2. UltramicroELISA para la detección de anticuerpos IgM anti M. leprae UltramicroELISA assay for the detection of human IgM antibodies to M. leprae

    OpenAIRE

    José Laferte; Elba G. Abreu; René Robaina; Vicente Verez

    1991-01-01

    La disponibilidad del sistema Ultramicroanalítico (SUMA) y de un antígeno especie-específico del M. leprae obtenido mediante síntesis química, permitió la normalización y validación de un ultramicroELISA para la detección de anticuerpos IgM específicos a esta micobacteria. El análisis de 433 sueros de banco de sangre y 265 sueros usados para validar el método y clasificados en un grupo control de donantes de banco de sangre (100), un grupo de pacientes tuberculosos (50), un grupo de enfermos ...

  3. Comparative evaluation of competitive ELISA test in Colombian cattle

    International Nuclear Information System (INIS)

    The main purpose of this study was to contribute to the definition of the best ELISA test to be used both for screening and differential diagnosis of Brucella abortus to be applied for control programmes

  4. [Indirect ELISA for the rapid diagnosis of Equine Influenza].

    Science.gov (United States)

    Vila Roza, M V; Galosi, C M; Oliva, G A; Echeverría, M G; Pecoraro, M R; Corva, S; Etcheverrigaray, M E

    2000-01-01

    An indirect enzyme linked immunosorbent assay was developed. Infected and non infected allantoic fluids precipitated with polyetilenglycol 6000 were used as antigen and control antigen, respectively. Serum samples were diluted 1/20 and a commercial horse radish peroxidase-labelled rabbit anti-equine IgG was used as second antibody. The reaction was developed using azino-diethylbenzotyazol-sulfonate (ABTS). Cut-off was determined by ratio sample (Rs). The hemagglutination inhibition test was used as a reference test for the 391 samples analyzed. Of these, 301 sera were positive by hemagglutination inhibition test and indirect ELISA, 75 were negative by both techniques, and 15 were positive by indirect ELISA and negative by hemagglutination inhibition test. Using hemagglutination inhibition test as standard, the indirect ELISA showed a relative specificity and sensitivity of 83.3 and 100%, respectively. This indirect ELISA is useful as screening test. PMID:10785942

  5. ELISA reader does not interfere by mobile phone radiofrequency radiation

    OpenAIRE

    Mortazavi, Seyyed Mohammad Javad; Baradaran-Ghahfarokhi, Hamid Reza; Abdi, Mohammad Reza; Baradaran-Ghahfarokhi, Milad; Mostafavi, Nayyer Sadat; Mahmoudi, Golshan; Berenjkoub, Nafiseh; Akmali, Zahra; Hossein-Beigi, Fahimeh; Arsang, Vajiheh

    2016-01-01

    Background: The increasing number of mobile phones can physically cause electromagnetic interference (EMI) in medical environments; can also cause errors in immunoassays in laboratories. The ELISA readers are widely used as a useful diagnostic tool for Enzymun colorimetric assay in medicine. The aim of this study was to investigate whether the ELISA reader could be interfered by the exposure to the 900 MHz cell phones in the laboratory. Materials and Methods: Human serum samples were collecte...

  6. PCR-ELISA detection of Escherichia coli in milk

    OpenAIRE

    Doyle, Sean; Daly, Paul; Collier, T.

    2002-01-01

    Aims: The purpose of this study was to develop a reliable molecular procedure for the detection of Escherichia coli in milk. Methods and Results: Robust and expeditious DNA extraction and PCR techniques were evaluated using Enzyme-Linked Immunosorbent Assay (ELISA) detection of biotin-labelled amplicons to facilitate optimal detection of E. coli DNA. Conclusions: It was found that 5 E. coli colony-forming units (cfu) could be detected per PCR reaction using the PCR-ELISA sys...

  7. Serodiagnosis of Oesophagostomum Columbianum infection in Goats using indirect ELISA

    Directory of Open Access Journals (Sweden)

    Fayaz Ahmad

    Full Text Available To determine the seroprevalence of Oesophagostomum columbianum antibodies in goats in Kashmnir, a study was conducted in Srinagar and Ganderbal Districts, located in central parts of Kashmir valley. ELISA was standardized and evaluated to detect goat Oesophagostomosis in experimental and clinical cases using somatic whole adult antigen of Oesophagostomum columbianum. Plate ELISA was standardized using 5μg/well antigen concentration with 1:100 and 1:1000 of sera and conjugate dilution. Indirect plate ELISA was able to demonstrate the antibody titer at different weeks post infection in experimental goats. A comparison of plate ELISA on suspected field sera and faecal sample examination by floatation method revealed that 68 (34.34% samples were found to be positive using ELISA and twelve by faecal examination. The highest prevalence was found in lower age groups in both the seasons, with the increase in age, the infection level decreased. The prevalence was higher in wet season as compared to dry season. The results were stastically significant (P < 0.05. No sex predisposition was seen. Sensitivity and specificity of ELISA was 80.0%, and 21.42%, respectively. This test is therefore quite sensitive for clinical cases; an early diagnosis, however lacks specificity of faecal examination were 10.81% and 90.0%, respectively. [Vet. World 2011; 4(11.000: 503-506

  8. Cadena del frío para la conservación de las vacunas en los centros de atención primaria de un área de Madrid: mantenimiento y nivel de conocimientos

    OpenAIRE

    Ortega Molina Paloma; Astasio Arbiza Paloma; Albaladejo Vicente Romana; Gómez Rábago M.ª Luisa; Juanes Pardo José Ramón de; Domínguez Rojas Vicente

    2002-01-01

    Fundamento. Las vacunas son medicamentos termolábiles y para garantizar su inmunogenicidad y eficacia protectora, dentro de los programas de inmunización, es imprescindible mantener la cadena de frío. El elemento fundamental en esta cadena es el personal responsable de las vacunas, que debe conocer las características de estabilidad de cada preparado con el fin de evitar errores durante su manipulación. El objetivo de este trabajo fue conocer cómo se realiza el mantenimiento de la cadena del ...

  9. Indirect ELISA for diagnosis of Brucella ovis infection in rams

    Directory of Open Access Journals (Sweden)

    S.A. França

    2014-12-01

    Full Text Available Brucella ovis is a major cause of epididymitis in sexually mature rams, resulting in subfertility, infertility, and economic losses for the sheep industry worldwide. The aim of this study was to develop an indirect ELISA (iELISA using recombinant proteins, namely rBoP59 and rBP26, as antigens for serological diagnosis of B. ovis infection. The BoP59 and BP26 recombinant proteins were expressed in E. coli and purified by affinity chromatography. Antigenicity was tested by Western blot and iELISA. Standardization of iELISA was performed with 500ng and 1µg BoP59 and rBP26 per well, testing serum from uninfected and experimentally infected rams. rBP26 was effective in distinguishing positive from negative rams. The rBP26 iELISA developed in this study is the first to use a completely purified rBP26 as antigen resulting in high sensitivity (100% and specificity (90.2%, and an overall accuracy equal to 1.0.

  10. Development of an ELISA for the Detection of Azaspiracids.

    Science.gov (United States)

    Samdal, Ingunn A; Løvberg, Kjersti E; Briggs, Lyn R; Kilcoyne, Jane; Xu, Jianyan; Forsyth, Craig J; Miles, Christopher O

    2015-09-01

    Azaspiracids (AZAs) are a group of biotoxins that cause food poisoning in humans. These toxins are produced by small marine dinoflagellates such as Azadinium spinosum and accumulate in shellfish. Ovine polyclonal antibodies were produced and used to develop an ELISA for quantitating AZAs in shellfish, algal cells, and culture supernatants. Immunizing antigens were prepared from synthetic fragments of the constant region of AZAs, while plate coating antigen was prepared from AZA-1. The ELISA provides a sensitive and rapid analytical method for screening large numbers of samples. It has a working range of 0.45-8.6 ng/mL and a limit of quantitation for total AZAs in whole shellfish at 57 μg/kg, well below the maximum permitted level set by the European Commission. The ELISA has good cross-reactivity to AZA-1-10, -33, and -34 and 37-epi-AZA-1. Naturally contaminated Irish mussels gave similar results whether they were cooked or uncooked, indicating that the ELISA also detects 22-carboxy-AZA metabolites (e.g., AZA-17 and AZA-19). ELISA results showed excellent correlation with LC-MS/MS analysis, both for mussel extract spiked with AZA-1 and for naturally contaminated Irish mussels. The assay is therefore well suited to screening for AZAs in shellfish samples intended for human consumption, as well as for studies on AZA metabolism. PMID:26245830

  11. Cellulose acetate as solid phase in ELISA for plague

    Directory of Open Access Journals (Sweden)

    Barbosa AD

    2000-01-01

    Full Text Available Antigen from Yersinia pestis was adsorbed on cellulose acetate discs (0.5 cm of diameter which were obtained from dialysis membrane by using a paper punch. ELISA for human plague diagnosis was carried out employing this matrix and was capable to detect amount of 1.3 µg of antigen, 3,200 times diluted positive serum using human anti-IgG conjugate diluted 1:4,000. No relevant antigen lixiviation from the cellulose acetate was observed even after washing the discs 15 times. The discs were impregnated by the coloured products from the ELISA development allowing its use in dot-ELISA. Furthermore, cellulose acetate showed a better performance than the conventional PVC plates.

  12. USO DE CADENAS DE MARKOV PARA LA PREDICCIÓN DE LA DINÁMICA DEL COMPORTAMIENTO DE PACIENTES EN UNA UNIDAD DE CUIDADO INTENSIVO CARDIOLÓGICA USE OF MARKOV CHAINS IN PREDICTION OF THE DYNAMICS OF PATIENT BEHAVIOR IN A CARDIOLOGIC INTENSIVE CARE UNIT

    Directory of Open Access Journals (Sweden)

    Víctor Albornoz

    2006-08-01

    Full Text Available En este trabajo presentamos un modelo probabilístico que contribuye al estudio de la dinámica en el comportamiento y permanencia de pacientes en una unidad de cuidados intensivos cardiológica. El modelo utilizado corresponde a una Cadena de Markov en tiempo discreto, que mediante la definición de determinados niveles de gravedad de un paciente (estados y la obtención de las correspondientes probabilidades de transición entre un nivel de gravedad y otro, permite predecir los tiempos de permanencia. Los diferentes estados empleados se basan en la construcción de un nuevo score creado para este propósito. Se muestran los detalles de la metodología adoptada y los principales resultados alcanzados en la aplicación del modelo empleado.In this paper we present a probabilistic model that contributes to the study of dynamics in the behavior and permanence of patients in a cardiovascular intensive care unit. The model corresponds to a discrete Markov Chain, that allows to predict the time that a patient remains in the system through the time, by means of certain severity of illness states and the corresponding transition probabilities between those states. The different states are based on the construction of a new score created for this study. We summarize the details of the adopted methodology and the main results reached in the application of the model.

  13. Comparative evaluation of competitive ELISA test in Colombian cattle

    International Nuclear Information System (INIS)

    In order to contribute to the definition of the best ELISA test for screening and differential diagnosis of Brucella abortus to be applied for control programmes, a total of 2971 sera from Colombian cattle were tested for brucellosis. Conventional agglutination tests, Buffered Plate antigen test (BPAT) and Rose Bengal (RB) as well as Complement Fixation test (CFT) (Alton, et al. 1988) were used comparatively. Radial immunodiffusion test (RID) was also performed to all sera. The sera were also tested using four different ELISAs: indirect ELISA from FAO/IAEA and the indirect ELISA modified by Nielsen, et al. 1992 as well as two competitive ELISAs: one competitive ELISA used B. abortus O-polysaccharide antigen and an enzyme conjugated monoclonal to the O-polysaccharide for competition and detection. The second competitive ELISA used lipopolysaccharide (sLPS) antigen, a different monoclonal antibody for competition but also specific for the O-polysaccharide and a commercially available goat anti-mouse IgG enzyme conjugate for detection. The sera were analyzed based on its population status, 987 positive obtained from Brucella abortus infected herds based on clinical and/or bacteriological evidence and a high prevalence of brucellosis, CFT percentage of positive animals in the herd was greater than 5%. Eight hundred sixty six (866) negative sera from non-vaccinated cattle from a brucellosis free area and 1118 negative sera obtained from reglamentary vaccinated areas under a free herd program. Initial cut-off values were derived using negative serum samples. The diagnostic sensitivity and specificity was defined from frequency histograms based on this cut-off values and using 2x2 tables, corresponding confidence limits (95%) were calculated. The data were also analysed using signal detection analysis (ROC). Kappa statistics was determined for all tests and populations, accuracy was used as index of comparison to evaluate different assays. The data support the initial

  14. Study of Immunoassay Methods for Recombinant Human Erythropoietin(rhEPO)Using Competitive ELISA

    Institute of Scientific and Technical Information of China (English)

    Jin YAN; Jie Bo MI; Wen Bao CHANG

    2004-01-01

    Two different immunoassay methods, competitive indirect enzyme-linked immuno- sorbent assay (CI-ELISA) and amplificative competitive indirect ELISA (ACI-ELISA) using biotin-avidin complex system were studied to detect rhEPO.The linear ranges were 50-20000 ng/mL and 10-50000 ng/mL for CI-ELISA and ACI-ELISA, respectively.The low detection limits of CI-ELISA and ACI-ELISA were 62.8 ng/mL and 8.5 ng/mL, respectively.

  15. Modelos para la planificación centralizada de la producción y el transporte en la cadena de suministro: una revisión

    OpenAIRE

    Josefa Mula; David Peidro; Manuel Díaz-Madroñero; JORGE E. HERNÁNDEZ

    2010-01-01

    Este artículo presenta una revisión bibliográfica sobre los modelos de programación matemática para la planificación centralizada de la producción y el transporte en una cadena de suministro. El propósito de esta revisión es identificar la investigación actual y futura en este campo y proponer un marco de clasificación basado en los elementos siguientes: estructura de la cadena de suministro, nivel de decisión, enfoque de modelado, propósito, información intercambiada y aplicación práctica...

  16. La influencia de la integración de la cadena de suministro en la implantanción de capacidades de comercio electrónico

    OpenAIRE

    José Moyano-Fuentes

    2010-01-01

    Este trabajo analiza la influencia de la integración de la cadena de suministro en la implantación de capacidades de comercio electrónico. Para ello, se parte de que la relación entre implantación de TICs y eficiencia está determinada por el uso de estas tecnologías y que existe una correlación positiva entre la vinculación de la estrategia de la cadena de suministro con la estrategia empresarial y la adopción de comercio electrónico. Los resultados obtenidos en la industria del automóvil señ...

  17. Estudio de los intercambiadores de cadenas utilizados en la abricación de cemento por vía húmeda

    Directory of Open Access Journals (Sweden)

    Papadakis, M.

    1964-12-01

    Full Text Available Not availableEl empleo de cadenas como intercambiador de calor es de uso muy extendido por su robustez y reducido gasto de entretenimiento. Su eficacia está comprobada. No obstante, ciertas dificultades que se presentan durante la marcha y, más acentuadas todavía, durante la puesta en servicio de un horno nuevo, demuestran que el problema no está aun completamente resuelto. Estas dificultades son de orden reológico. Este estudio tiene por objeto proporcionar un método que permite calcular la repartición y dimensionado óptimo de las cadenas teniendo en cuenta las propiedades reológicas de la pasta. El aspecto térmico de la cuestión parece, en efecto, mucho menos importante.

  18. ANÁLISIS DE VARIABLES ESTRATÉGICAS PARA LA CONFORMACIÓN DE UNA CADENA PRODUCTIVA DE QUINUA EN COLOMBIA

    Directory of Open Access Journals (Sweden)

    Luz Alexandra Montoya Restrepo

    2005-12-01

    Full Text Available En este artículo se presenta una síntesis de la investigación realizada por el Grupo Interdisciplinario Biogestión sobre los usos y la producción de quinua en Colombia. Se ofrece un análisis estratégico del sector quinuero en el país a fin de identificar los factores clave de cambio que tienen un moderado o alto grado de gobernabilidad y que podrían afectar la conformación de la cadena productiva de quinua. También se proponen algunas estrategias orientadas a controlar estos factores de cambio para lograr la adecuada conformación de la cadena.

  19. El papel de las TIC en el rendimiento de las cadenas de suministro: el caso de las grandes empresas de España y Portugal

    Directory of Open Access Journals (Sweden)

    Nicolás Gonzálvez- Gallego

    2010-01-01

    Full Text Available Este artículo analiza las diferencias existentes entre las grandes empresas de España y Portugal en lo referido tanto al uso de las Tecnologías de la Información y la Comunicación (TIC como a la integración electrónica con los agentes de la cadena de suministro. Estas diferencias se establecen en términos de la capacidad para generar resultados empresariales superiores. Los resultados muestran diferencias entre ambos grupos de empresas y confirman que las empresas españolas deberían perseguir una mayor integración con la cadena de suministro, por ejemplo, a través del desarrollo de TIC que faciliten la colaboración con clientes y proveedores.

  20. INNOVACIÓN TECNOLÓGICA DE LA CADENA AGROALIMENTARIA DE MAÍZ PARA MEJORAR SU COMPETITIVIDAD: ESTUDIO DE CASO EN EL ESTADO DE HIDALGO

    OpenAIRE

    Georgel Moctezuma-López; José Antonio Espinosa-García; Venancio Cuevas-Reyes; José Luis Jolalpa-Barrera; Fernando Romero-Santillán; Alejandra Vélez-Izquierdo; Diana Elisa Bustos Contreras

    2010-01-01

    El objetivo de esta investigación fue identificar la oferta y demanda tecnológica para plantear alternativas en la innovación de la cadena agroalimentaria de maíz en el estado de Hidalgo, México. Se realizaron las siguientes etapas: 1) caracterización de la cadena; 2) identificación de los factores críticos; 3) distinción de demandas tecnológicas de conocimiento y política; 4) reconocimiento de la oferta tecnológica y 5) cuantificación de proyectos de investigación y política pública. Se apli...

  1. Técnicas para el Modelado de Procesos de Negocio en Cadenas de Suministro Business Process Modelling Techniques in Supply Chains

    Directory of Open Access Journals (Sweden)

    Raquel Sanchis

    2009-01-01

    Full Text Available El presente artículo muestra una revisión y evaluación de las técnicas y metodologías genéricas de modelado de procesos de negocio más populares y utilizadas en la literatura. El principal objetivo del estudio es la propuesta de una taxonomía de clasificación de las técnicas de modelado aplicadas a las características propias de las cadenas de suministro, para facilitar la selección de las más apropiadas. Para verificar la clasificación, se describe un caso real de estudio en cadenas de suministro, en el que se relaciona una cadena de suministro del sector cerámico y una del sector del mueble. Del estudio realizado, se propone un método que incluye cuatro técnicas para la representación de las diferentes perspectivas de modelado en una cadena de suministro.The article shows a review and evaluation of the most popular and meaningful business process modelling techniques and generic methodologies treated in the literature. The main objective is the proposal of a new taxonomy of classification oriented to supply chains, with the aim of facilitating the selection of the most appropriate ones. To verify the classification, a real study case of supply chains is described. In this study, a supply chain from the ceramic sector is related with one from the furniture sector and a method consisting of four modelling techniques to present the different aspects to be considered for representing the different perspectives of the supply chains is proposed.

  2. Diagnóstico molecular de Campylobacter en la cadena avícola destinada para consumo humano en Costa Rica.

    Directory of Open Access Journals (Sweden)

    Leana Zumbado-Gutiérrez

    2014-07-01

    Full Text Available El objetivo de este estudio fue determinar la presencia y la frecuencia de Campylobacter jejuni y Campylobacter coli, en la cadena de producción avícola en Costa Rica. Se evaluaron granjas, planta de proceso y puntos de venta del Gran Área Metropolitana. Para la detección y aislamiento de Campylobacter sp. se analizaron 84 muestras provenientes de la cadena avícola (24 muestras de carne de pollo tomadas en punto de venta, 20 enjuagues de carcasa y 40 de ciegos tomados en planta de beneficio, obtenidas en noviembre de 2012, siguiendo el protocolo ISO 10272-1:2006 modificado por el Departamento de Agricultura de Estados Unidos (USDA, por sus siglas en inglés. De las 84 muestras analizadas, 36 (42,8% resultaron positivas para C. jejuni, y una (1,2% para C. coli. La cantidad de muestras positivas según la Reacción en Cadena de Polimerasa (PCR, por tipo de muestra fue: para contenido cecal, 16 (n=40, para carcasas, 8 (n=20 y para punto de venta 12 (n=24. Dieciséis de las veinte granjas muestreadas fueron positivas. Hubo una alta frecuencia de Campylobacter en todos los puntos de la cadena muestreados, lo que podría estar asociado a modificaciones de parámetros relacionados con inocuidad en cada eslabón. El enfriamiento rápido y el uso de cloro en el agua en la planta de proceso, disminuyó la frecuencia de muestras positivas de Campylobacter spp.

  3. Elisas käärivad sisemised pinged / Sirje Rank

    Index Scriptorium Estoniae

    Rank, Sirje, 1966-

    2007-01-01

    Soome sidefirma Elisa nõukogu on vastu firma suuraktsionäri Thor Björgolfssoni nõudmistele ettevõte tükeldada ning käivitada välisturgudel uus ja agressiivne kasvustrateegia. Lisa: Thor Björgolffson

  4. Detection of antibodies to the 20s proteasome by ELISA

    DEFF Research Database (Denmark)

    Jørgensen, Karin Meinike; Frederiksen, Jette Lautrup; Nielsen, Christoffer Tandrup;

    2013-01-01

    The presence of antibodies against the 20S proteasome has been correlated with diseases like multiple sclerosis (MS) and systemic lupus erythematosus (SLE) but no definite association has been established. In order to investigate this further, we optimized an ELISA for proteasome antibodies and...

  5. Rapid screen for truncating ATM mutations by PTT-ELISA

    Energy Technology Data Exchange (ETDEWEB)

    Du Liutao; Lai, C.-H. [Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, CA 90095 (United States); Concannon, Patrick [Department of Biochemistry and Molecular Genetics, University of Virginia, VA 22908 (United States); Gatti, Richard A. [Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, CA 90095 (United States); Department of Human Genetics, David Geffen School of Medicine at UCLA, CA 90095 (United States)], E-mail: rgatti@mednet.ucla.edu

    2008-04-02

    Mutations in the ataxia-telangiectasia mutated (ATM) gene are responsible for the autosomal recessive genetic disorder, ataxia-telangiectasia (A-T). Approximately 80% of ATM mutations found in A-T patients results in truncations, which can be detected by Protein Truncation Test (PTT). Conventional PTT uses SDS-PAGE electrophoresis to detect mobility of radiolabeled truncated protein fragments. In this study, we developed a non-radioactive Protein Truncation Test which utilizes an enzyme-linked immunosorbent assay (PTT-ELISA) to detect ATM mutations in eight overlapping fragments. N- and C-terminal epitopes (c-myc and V5, respectively) were introduced into transcription/translation products, which could then be detected by Sandwich ELISA. Using this assay, we screened 9 newly diagnosed A-T patients consecutively. Of the 18 expected mutations, 14 truncating mutations were independently identified by cDNA direct sequencing and/or DNA dHPLC analysis. PTT-ELISA detected all of these 14. Four mutations were novel. The PTT-ELISA provides a rapid method for detecting truncating mutations in large genes and should be considered prior to using more laborious or costly methods, such as direct sequencing.

  6. Contexto y Caracterización de la Cadena de Suministro del Limón Persa (Citrus latifolia Tanaka en Veracruz-México

    Directory of Open Access Journals (Sweden)

    Gregorio Fernández-Lambert

    2015-01-01

    Full Text Available Esta investigación se realiza en el Distrito Citrícola de Martínez de la Torre, Ver., con alcance a los municipios de Álamo Temapache, y Cuitláhuac en Veracruz, y reporta el contexto e integración de la cadena de suministro del limón persa. El estudio de sus eslabones permitió identificar las problemáticas de abastecimiento, operación y distribución en dicha cadena. A partir de entrevistas abiertas no estructuradas a especialistas, técnicos, productores y empresarios, así como la consulta de bases de datos y documentos de organismos afines a esta agroindustria, los resultados de la investigación advierten que la fuente de los problemas estructurales de la ineficacia en la sincronización de la cadena de suministro del limón persa, se centra en la baja productividad del huerto, un excesivo intermediarismo entre el huerto y la exportadora, la baja adopción tecnológica o uso de tecnología hechiza en los procesos de empaque del cítrico.

  7. Modelos para la planificación centralizada de la producción y el transporte en la cadena de suministro: una revisión

    Directory of Open Access Journals (Sweden)

    Josefa Mula

    2010-08-01

    Full Text Available Este artículo presenta una revisión bibliográfica sobre los modelos de programación matemática para la planificación centralizada de la producción y el transporte en una cadena de suministro. El propósito de esta revisión es identificar la investigación actual y futura en este campo y proponer un marco de clasificación basado en los elementos siguientes: estructura de la cadena de suministro, nivel de decisión, enfoque de modelado, propósito, información intercambiada y aplicación práctica. El objetivo del artículo es proporcionar un punto de partida para el estudio de los problemas de planificación de la producción y el transporte de la cadena de suministro orientado a los investigadores de gestión de la producción.

  8. Propuesta de un procedimiento para el diagnóstico de la cadena de suministro de la Empresa Pesquera de Sancti Spíritus.

    Directory of Open Access Journals (Sweden)

    DamarisTaydi Castillo Jiménez

    2015-12-01

    Full Text Available La presente investigación se desarrolla con el objetivo de proponer un procedimiento para diagnosticar la cadena de suministro. En consecuencia se propone la metodología del Modelo SCOR para dar cumplimiento al objetivo propuesto. El mismo se aplica en la Empresa Pesquera de Sancti Spíritus (PESCASPIR.Los resultados del diagnóstico permitieron identificar aquellas oportunidades de mejora que mayor incidencia tienen sobre el desempeño de la cadena, lográndose además un marco único que une los procesos de negocio, los indicadores de gestión, las mejores prácticas y las tecnológicas, en una estructura unificada para mejorar la comunicación entre los miembros de la cadena de suministros. Para el estudio se utilizaron técnicas de análisis, de recopilación de información, organigramas y otros.

  9. Análisis de la capacidad de servicio de mantenimiento de la cadena TRD Caribe Sancti Spíritus usando la simulación.

    Directory of Open Access Journals (Sweden)

    Manuel Hung Varela

    2014-09-01

    Full Text Available La investigación, tiene su origen en la necesidad de conocer, si la unidad de servicio de mantenimiento en la Cadena TRD Caribe Sancti Spíritus, es capaz de satisfacer o no las demandas de la Cadena, ya que se presenta una tendencia al crecimiento en la demanda, así como en las quejas por parte de los clientes que aseveraban largas esperas para ser servidos. Se definió el procedimiento para la simulación por eventos discretos a utilizar (Barceló Bugeda, 1996. Se definieron las fronteras del sistema, se construyó el modelo matemático para el sistema objeto de estudio y se programó en el software especializado Promodel 4.0. Para la simulación del modelo matemático, se recopiló información a través de técnicas como el muestreo aleatorio. El modelo fue verificado y validado (Barceló Bugeda, 1996; Aracil Santonja, 1995. Se simuló el modelo, haciendo uso del método de los lotes. Se procesaron los resultados de la simulación, y se concluyó que la unidad de servicio de mantenimiento no dispone de capacidad suficiente bajo las condiciones actuales para satisfacer las demandas de la cadena TRD Caribe en el territorio de Sancti Spíritus.

  10. Zebragryllus Desutter-Grandcolas & Cadena-Casteñada, n.gen. a new Gryllinae genus from Eastern and Western Amazonia, South America (Orthoptera, Grylloidea, Gryllidae).

    Science.gov (United States)

    Desutter-Grandcolas, Laure; Cadena-Castañeda, Oscar J; Jaiswara, Ranjana; Anso, Jeremy

    2014-01-01

    We describe a new genus of grylline cricket, Zebragryllus Desutter-Grandcolas & Cadena-Casteñada n. gen., from the Neotropical Region, using characters of morphology and male genitalia; genitalic characters clearly show that Zebragryllus n. gen. is closely related to Anurogryllus Saussure, 1878. Six species are described as new to science, originating from western (Peru, Colombia) and eastern (French Guiana) Amazonia: Zebragryllus fuscus Desutter-Grandcolas, n. sp., Z. guianensis Desutter-Grandcolas, n. sp., Z. intermedius Desutter-Grandcolas, n. sp., Zebragryllus nauta Desutter-Grandcolas, n. sp., Zebragryllus nouragui Desutter-Grandcolas, n. sp., and Zebragryllus wittoto Desutter-Grandcolas and Cadena-Casteñada, n. sp., type species of the genus. They are characterized by their size, coloration (shining black, most often with white patterns of coloration, hence the genus name), and male and female genitalia. The calling songs of Z. guianensis Desutter-Grandcolas, n. sp., Z. intermedius Desutter-Grandcolas, n. sp., Z. nouragui Desutter-Grandcolas, n. sp., and Z. wittoto Desutter-Grandcolas and Cadena-Casteñada, n. sp. are described. An identification key is proposed for both males and females. PMID:24871163

  11. ¿Cómo aplicar la flexibilidad administrativa en las entidades de educación superior mediante el sistema de costos abc?

    Directory of Open Access Journals (Sweden)

    Dilia Castillo

    2005-10-01

    Full Text Available El método de Costos Basado en Actividades se ha creado para generar en las organizaciones, estructuras administrativas flexibles mediante la clasificación de las actividades (cadena valor, en centros primarios y de apoyo. Este sistema permite la acumulación de costos en forma directa en cada dependencia responsable de desarrollar tareas que enconjunto originan que una organización ofrezca a sus clientes un producto terminado o un servicio eficiente a un costo real previamente determinado. Las tareas producidas por una causa común son agrupadas en pools, minimizando aquellos factores que no generen valor agregado. Para la implementación de este sistema de costos es necesario llevar a cabo cinco etapas: definición y clasificación de actividades primarias y de apoyo, definición de los inductores de costo, realización del reparto primario y la acumulación de costos en los centros de actividad, realización del reparto secundario en los centros de actividad primarios y presentación de los resultados por cada actividad principal. El sistema de costos ABC, es una alternativa para el manejo de la flexibilidad en la administración de una entidad de educación superior, y supera el problema de la sub. o sobre-aplicación de los costos totales en cada actividad necesaria para la prestación del servicio.

  12. A sandwich ELISA for the detection of Wnt5a.

    Science.gov (United States)

    Kummitha, China Malakondaiah; Mayle, Kristine M; Christman, Mark A; Deosarkar, Sudhir P; Schwartz, Anthony L; McCall, Kelly D; Kohn, Leonard D; Malgor, Ramiro; Goetz, Douglas J

    2010-01-31

    Wnt5a is a noncanonical member of the Wnt family of signaling molecules that has been linked to various physiological and pathological processes including cell differentiation, cell migration, cell growth, vascular remodeling, cancer and chronic inflammation. To understand the role of Wnt5a in these processes, it is necessary to determine the function and expression level of Wnt5a. In this study we developed a sensitive and specific sandwich enzyme-linked immunosorbent assay (ELISA) for detecting Wnt5a. We found that a rabbit anti-human Wnt5a is a suitable capture antibody for establishing a sandwich ELISA. We used two systems to detect Wnt5a: (1) goat anti-mouse Wnt5a and horseradish peroxidase (HRP) conjugated F(ab')(2) donkey anti-goat IgG as detection and enzyme-linked antibodies respectively, or (2) biotinylated goat anti-mouse Wnt5a and HRP-streptavidin as detection antibody and enzyme-linked avidin respectively. A sandwich ELISA using either of these systems failed to detect recombinant mouse (rm)-Wnt5a diluted in Hank's balanced salt solution supplemented with Ca(2+) and Mg(2+) and 1% bovine serum albumin (HBBS+, 1% BSA). Addition of polyethylene glycol (PEG) to the HBBS+, buffer during the binding stage of rm-Wnt5a, afforded the detection of rm-Wnt5a. The use of PEG during both the binding of rm-Wnt5a and detection antibody stages of the assay yielded the maximum signal for rm-Wnt5a. The relationship between the ELISA signal and concentration of Wnt5a was linear with an R(2) of 0.9934. In summary, we have developed a specific and sensitive sandwich ELISA that detects rm-Wnt5a. PMID:19919840

  13. Modelos para el análisis de los negocios inclusivos: construcción mediante el estudio de casos

    Directory of Open Access Journals (Sweden)

    Oscar D. Licandro

    2013-01-01

    Full Text Available El concepto de Negocios Inclusivos (NI con la Base de la Pirámide (BDP ha sido formulado para describir y sistematizar un conjunto de experiencias surgidas en los años 2000, orientadas a generar inclusión social mediante la creación de emprendimientos asociativos de personas pobres, incorporados a la cadena de valor de empresas. El desarrollo teórico y la investigación empírica sobre esta estrategia de inclusión económica y social son todavía incipientes. No obstante ello, el análisis de un amplio abanico de experiencias ha permitido elaborar algunas herramientas conceptuales útiles para orientar la reflexión teórica, la investigación empírica y el análisis estratégico de este tipo de negocio. Este documento tiene como objetivo presentar algunas de esas herramientas que han sido diseñadas en base al estudio de casos de NI en Uruguay. El documento parte de una revisión bibliográfica sobre el tema, que recorre los principales abordajes teóricos (Prahalad y Hammond, 2005; Austin y Chu, 2006; AVINA, 2010; Casado, 2011; Simanis et al., 2008 e investigaciones empíricas basadas en el estudio de casos (PNUD, 2008; Marquez et al., 2010. Luego, fundamenta la pertinencia del enfoque de NI como nueva estrategia para abordar el problema de la pobreza, formula una breve reseña de la evolución del concepto y culmina con la descripción de cinco herramientas conceptuales elaboradas a partir del estudio de 16 casos en Uruguay. Este estudio se realizó mediante entrevistas a distintos actores que participaron en el proceso de creación de cada uno de los emprendimientos, el análisis de documentos internos de los emprendimientos y páginas web y la revisión de artículos de prensa.

  14. Relación entre la prueba intradermica de histoplasmina y los niveles de anticuerpos detectables por ELISA e immunodifusion Relationship between histoplasmin skin test and Histoplasma capsulatum antibody levels detected by ELISA and immunodiffusion tests

    Directory of Open Access Journals (Sweden)

    Carlos M. Fernandez-Andreu

    1994-02-01

    Full Text Available Se realizó un estudio prospectivo en 40 trabajadores de una granja avícola (grupo 1 considerados con riesgo de exposición a Histoplasma capsulatum, agente etiológico de la histoplasmosis, y en 16 individuos sin riesgo profesional de exposición a dicho agente (grupo 2. En ambos grupos se aplicó la prueba intradérmica de histoplasmina y se obtuvo el suero antes de su aplicación y a los 30 y 180 días después de realizada dicha prueba. Se determinó el nivel de anticuerpos anti-H. capsulatum mediante las técnicas de ELISA e inmunodifusión doble. En los dos grupos de población estudiados la aplicación intradérmica de histoplasmina, aún en los casos en que la respuesta fue positiva, no constituyó un estímulo antigénico suficiente para provocar un aumento en los niveles de anticuerpos anti-H. capsulation detectables por las técnicas serológicas empleadas. Los resultados obtenidos contribuyen a la mejor interpretación de la prueba de ELISA en el diagnóstico de la histoplasmosis.A prospective study was carried out in two groups of individuals: a group 1 (n=40 included workers from a poultry farm, with potencial occupational risk of exposure to Histoplasma capsulatum, ethiologic agent of histoplasmosis, and a group 2 (n=16, persons without occupational risk of exposure to the agent. Histoplasmin skin test was performed in both groups, and three sera were obtained from each individual: 1 before skin test was done, 2 30 days after, and 3 180 days after it. In both groups the histoplasmin skin test, even when the test was positive, was not a sufficient antigenic booster to provoque an increase in the H. capsulatum antibody levels capable to be detected by the Serologic tests used (ELISA and Double Immunodiffusion. These results contribute to improve the interpretation of ELISA test values in the diagnosis of histoplasmosis.

  15. UltramicroELISA para la detección de anticuerpos IgM anti M. leprae UltramicroELISA assay for the detection of human IgM antibodies to M. leprae

    Directory of Open Access Journals (Sweden)

    José Laferte

    1991-12-01

    Full Text Available La disponibilidad del sistema Ultramicroanalítico (SUMA y de un antígeno especie-específico del M. leprae obtenido mediante síntesis química, permitió la normalización y validación de un ultramicroELISA para la detección de anticuerpos IgM específicos a esta micobacteria. El análisis de 433 sueros de banco de sangre y 265 sueros usados para validar el método y clasificados en un grupo control de donantes de banco de sangre (100, un grupo de pacientes tuberculosos (50, un grupo de enfermos de lepra (65 y un grupo de contactos de estos enfermos (50, mostró la especificidad del ensayo para evidenciar la infección con el M. leprae. Los resultados obtenidos del estudio adicional de 140 muestras de suero de contactos de enfermos estuvieron estrechamente correlacionados (r = 0,98 con los resultados obtenidos por la técnica de microELISA convencional. La utilización del SUMA no solo permite un notable ahorro de reactivos si no además facilita la lectura, cálculo, validación y almacenamiento automático de los resultados.The availability of an ultramicroanalitic system (SUMA and specie-specific antigen of M. leprae obtained by chemical synthesis, have made possible the standardization and validation of an ultramicroELISA assay for detecting specific human IgM antibodies to this mycobacterium. The specificity of this test to demonstrate the infection with M. leprae was corroborated through a screening of 433 blood bank serum samples and other 265 from diferent groups (100, control group, 50 tuberculosis patients, 65 leprosy patients, 50 from household. The results obtained in the aditional study of 140 household sero showed a high correlation (r = 0.98 with the conventional microELISA method. The use of SUMA allows saving reagents and time since sample handling, plate reading, print out and storing the data are computer assisted.

  16. E.L.I.S.A. en coccidioidomicosis humana E.L.I.S.A. in human coccidioidomycosis

    Directory of Open Access Journals (Sweden)

    Iris Nora Tiraboschi

    1991-08-01

    Full Text Available Se realizó E.L.I.S.A. con exoantígeno de Coccidioides immitis para la detectión de anticuerpos, en 67 sueros humanos diluidos 1/1000, 1/2000, 1/4000 y 1/8000. De los 18 sueros de enfermos de coccidioidomicosis comprobada por examen directo, cultivo y/o histología, 5 fueron negativos, en otros 13 fueron positivos en una o varias diluciones. 3/26 sueros de personas sanas, coccidioidino positivas, fueron positivos en títulos de 1/1000 y el resto no tuvo anticuerpos detectables. No presentaron reacciones positivas ninguno de los sueros controles de personas sanas, pero sí lo hicieron 4/8 pacientes con otras micosis. Se concluye que E.L.I.S.A. es útil para la detección de mínimas cantidades de anticuerpos o en sueros que no pueden ser procesados por fijación de complemento. No es recomendable el uso de la técnica en forma aislada por al presencia de reacciones cruzadas.An E.L.I.S.A. test for antibody detection, with an exo-antigen of Coccidioides immitis was standardized in 67 humans sera diluited in 1/1000, 1/2000, 1/4000 and 1/8000. Eightheen sera from mycologically proved cases of coccidioidomycosis were studied: 5 were negative and 13 were positive in some dilutions. 3/26 sera of healthy persons who presented positive skin tests with coccidioidin were positive and the other 23 sera did not have positive reactions. None of the 15 sera of healthy human exhibited positive E.L.I.S.A. Serum samples of 8 patients suffering other deep mycosis were studied, 4 of them presented cross-reactions in E.L.I.S.A. tests. E.L.I.S.A. test seems to be a useful Serologic technique for antibody detection in anticomplementary serum samples or when a low concentration of antibodies should be detected. As it is very sensitive, cross-reactions with other mycoses are frequent, thus the use other more specific serologic technique together E.L.I.S.A. is recommended.

  17. FUNCIONALIDADES DE SISTEMAS DE PLANIFICACIÓN DE RECURSOS EMPRESARIALES PARA CADENAS DE SUMINISTRO / FUNCTIONALITIES OF ENTERPRISE RESOURCE PLANNING SYSTEMS FOR SUPPLY CHAIN INTEGRATION

    Directory of Open Access Journals (Sweden)

    Dania Pérez-Armayor

    2013-05-01

    Full Text Available En la actualidad los sistemas de planificación de recursos empresariales, además de facilitar las relaciones y la eficiencia intra-empresarial, incluyen elementos para viabilizar la colaboración entre empresas, como respuesta a la necesidad de las organizaciones de agruparse en cadenas de suministro. En consecuencia, el Ministerio de la Informática y las Comunicaciones, junto a otras organizaciones cubanas, están proyectando el desarrollo de un sistema de planificación de recursos empresariales desarrollado en el país (Sistema Cedrux, incluyendo funcionalidades que soporten la integración de cadenas de suministro. Este trabajo se centra en el análisis de funcionalidades que debe poseer el Sistema Cedrux para facilitar una gestión de la cadena de suministro exitosa. Para complementar este análisis fueron realizadas búsquedas bibliográficas y entrevistas a personal participante en el desarrollo de Cedrux. Como resultado se propone la configuración de los módulos acorde al rol de la empresa en la cadena, incluyendo módulos de gestión de cadenas de suministroAbstractNowadays, enterprise resource planning systems (ERP systems, in addition to help improving the relations and the intra-enterprise efficiency, include elements that facilitate inter-enterprise cooperation in response to the need for organizations to come together in supply chains. As a consequence, the Ministry of Informatics and Communications, along with other Cuban organizations, are planning the development of an ERP system developed in Cuba (named Cedrux system, which includes functionalities that support the integration of supply chains. This paper aims to analyze the functionalities that Cedrux should have in order to support a successful supply chain management. Bibliographic searches and interviews to Cedrux developers were conducted, in order to complement this analysis. As a result is suggested the inclusion of modules for supply chain management and the

  18. Cadenas agroalimentarias orgánicas en el sur de chile: tensiones que condicionan su puesta en valor

    Directory of Open Access Journals (Sweden)

    Sandra Ríos Núñez

    2016-01-01

    Full Text Available El modelo industrial de producción de alimentos ha incentivado la emergencia de cadenas agrarias alternativas. Estas suponen modelos de producción fuertemente vinculados al territorio y tienen la finalidad de transitar desde la producción industrial a la captura de valor por medio de sistemas de producción que garanticen la seguridad y soberanía alimentaria. Esta investigación analiza el funcionamiento de la agricultura orgánica en Chile, en tanto producción agraria no convencional, con la finalidad de perfilar su desarrollo como una estrategia de captura de valor y producción de alimentos saludables especialmente para pequeños pro- ductores agrarios. Se busca visibilizar modelos de producción que reconectan el flujo de producción-circulación-consumo a través de un mecanismo de gobernanza que posibilite la captura de valor en el territorio.

  19. MODELO MATEMÁTICO PARA LA PLANIFICACIÓN DE LA PRODUCCIÓN EN LA CADENA DE SUMINISTRO

    Directory of Open Access Journals (Sweden)

    Caridad González Sánchez

    2004-07-01

    Full Text Available

    La planificación de la producción dentro de la cadena de suministro es una actividad de especial importancia en estos momentos, debido a que permite la optimización de las operaciones de producción y distribución, y satisfacer la mayoría de los pedidos de los clientes en el tiempo requerido y a un costo más bajo. La aplicación de las técnicas matemáticas en este proceso garantiza una toma de decisiones más rápida y eficiente. En el presente trabajo se propone un modelo matemático multiobjetivo que permite satisfacer las demandas de los clientes determinando la cantidad de recursos necesarios para llevar a cabo la producción lo que redundará en la disminución de los niveles de inventarios.

  20. Detection of cow milk adulteration in yak milk by ELISA.

    Science.gov (United States)

    Ren, Q R; Zhang, H; Guo, H Y; Jiang, L; Tian, M; Ren, F Z

    2014-10-01

    In the current study, a simple, sensitive, and specific ELISA assay using a high-affinity anti-bovine β-casein monoclonal antibody was developed for the rapid detection of cow milk in adulterated yak milk. The developed ELISA was highly specific and could be applied to detect bovine β-casein (10-8,000 μg/mL) and cow milk (1:1,300 to 1:2 dilution) in yak milk. Cross-reactivity was milk. The linear range of adulterant concentration was 1 to 80% (vol/vol) and the minimum detection limit was 1% (vol/vol) cow milk in yak milk. Different treatments, including heating, acidification, and rennet addition, did not interfere with the assay. Moreover, the results were highly reproducible (coefficient of variation milk adulterated with cow milk. PMID:25151876

  1. A Sandwich ELISA for the Detection of Wnt5a

    OpenAIRE

    Kummitha, China Malakondaiah; Mayle, Kristine M.; Christman, Mark A.; Deosarkar, Sudhir P.; Schwartz, Anthony L; McCall, Kelly D.; Kohn, Leonard D.; Malgor, Ramiro; Goetz, Douglas J.

    2009-01-01

    Wnt5a is a noncanonical member of the Wnt family of signaling molecules that has been linked to various physiological and pathological processes including cell differentiation, cell migration, cell growth, vascular remodeling, cancer and chronic inflammation. To understand the role of Wnt5a in these processes, it is necessary to determine the function and expression level of Wnt5a. In this study we developed a sensitive and specific sandwich enzyme-linked immunosorbent assay (ELISA) for detec...

  2. Validación y aplicación de la prueba ELISA para medir cortisol fecal en jaguar (Panthera onca y puma (Puma concolor durante un programa de enriquecimiento ambiental en el Zoológico Jaime Duque

    Directory of Open Access Journals (Sweden)

    Catalina Rodríguez Álvarez

    2005-12-01

    Full Text Available El enriquecimiento ambiental busca aumentar el bienestar de los animales cautivos mediante la provisión de estímulos que motiven la realización de comportamientos típicos de la especie. A las poblaciones de jaguares (Panthera onca y pumas (Puma concolor presentes en el Zoológico Jaime Duque se les aplicó un programa de enriquecimiento ambiental y con el fin de probar si su bienestar aumentaba, se realizaron mediciones de cortisol en materia fecal mediante la prueba de ELISA, para lo cual hubo necesidad de validar la técnica para cada especie. La prueba utilizada resultó ser válida para ambas especies; sin embargo, los niveles de cortisol se ven influenciados por múltiples variables y no se hizo evidente la reducción de los niveles del mismo con el enriquecimiento ambiental.

  3. The FAO/IAEA rinderpest indirect ELISA kit

    International Nuclear Information System (INIS)

    To meet the needs of the sero-monitoring component of the Pan African Rinderpest Campaign, the Joint FAO/IAEA Division in conjunction with the Pirbright Laboratories of the Institute for Animal Health, UK, developed an ELISA kit for the detection of antibodies to rinderpest virus in cattle. This kit was specifically designed to be used in the sort of conditions likely to be met in laboratories in Africa. The kit was based on an indirect ELISA and contained all the necessary reagents to test 20,000 cattle sera in duplicate. The kit also contained a detailed protocol (reproduced in this document) describing how to carry out and interpret the results of the test, a list of all the reagents and how to prepare and store them and information on the types of problems likely to be encountered in using the kit and how to solve them. The FAO/IAEA rinderpest ELISA kit has now been used to test over a quarter of a million cattle sera and has been shown to be a robust and reliable system for the sero-monitoring of rinderpest in Africa. (author). 7 refs, 2 figs

  4. The ELISA technique for the detection and evaluation of rinderpest

    International Nuclear Information System (INIS)

    As part of the Pan African Rinderpest Campaign, sero-surveillance is required to monitor the success of the national vaccination programmes and to assist in identifying foci of infection in the latter stages of the campaign. The FAO/IAEA ELISA based system for rinderpest sero-monitoring was successfully introduced into the National Veterinary Institute, Debre Zeit. The ELISA was compared with the virus neutralization test and found to give comparable results (94% agreement). For routine use of the assay a local negative value for cattle was established and subsequently this local negative control was used in the testing of all field sera. A sampling frame was prepared in such a manner that results derived from the sera collected would be representative of the country as a whole. At the time of sampling, detailed information on the animals being tested was obtained and submitted to the National Veterinary Institute along with the sera for testing. In the initial survey, 15,000 sera were collected from throughout the country and to-date, 9,000 tested by the ELISA for the presence of antibodies to rinderpest virus. Overall, 76% of the animals were found to be immune although considerable cocal variation occurred. The surveillance system proved to be an invaluable tool in monitoring the vaccination programme and providing the rinderpest national coordinator with detailed information on the success of the programme on a district to district basis and thus pinpointing areas where revaccination was required. (author). 3 refs, 1 fig., 2 tabs

  5. Influence of affinity on antibody determination in microtiter ELISA systems

    International Nuclear Information System (INIS)

    Theoretically, all immunoassays are affinity (Ka) dependent when the product of the antibody (Ab) Ka and the free epitope concentration is less than 10. Thus, the degree of dependence on Ka depends on the concentration of available antigen in the system. The authors examined the binding of 125I-anti-fluorescein (a-FLU) monoclonal antibodies of different affinities to FLU-gelatin adsorbed on Immunlon 2 microtiter plates. Data obtained were in general agreement with our theoretical predictions; the percent of 125I-a-FLU which bound correlated with Ka, as did the shape of the titration curves. Measurement of 5 a-FLU monoclonals by the ELISA showed that the determination of Ab concentrations depends on the FLU-gelatin concentration, epitope density, and on the relationship between the Kas of test samples and the reference standard Ab preparation. Thus the ELISA is Ka dependent and should not be used routinely to estimate the absolute amount to Ab in unknown samples. However, the Ka dependency of the ELISA might provide a convenient assay for the estimation of the relative functional Ka (rfKa) of antibody preparations

  6. Utilidad de sangre almacenada en papel de filtro para estudios serologicos por ELISA de inhibicion Use of filter paper strips on an ELISA inhibition test for serologic studies on dengue

    Directory of Open Access Journals (Sweden)

    S. Vázquez

    1991-08-01

    Full Text Available Para evaluar la utilidad del método de recolección de muestras de sangre en papel de filtro para la detección de anticuerpos anti-dengue mediante un ELISA de Inhibición recientemente desarrollado en nuestro laboratorio, se realizó una toma simultánea de muestras de sangre en papel de filtro y de suero, de donantes de Banco de Sangre. Ambas muestras fueron conservadas a -20ºC y probadas a los 15 dias, 3 y 6 meses respectivamente. A las muestras que resultaron positivos se les amplió el rango de dilución para determinar título. Al realizar la comparación entre ambas muestras, sangre en papel de filtro y suero, encontramos que no existían diferencias de detección significativas, tanto para los casos positivos como los negativos. No obstante se observó en ambas muestras y de forma general una disminución del titulo de anticuerpos (una dilución al transcurrir el tiempo máximo establecido en nuestro estudio (6 meses.To evaluate the usefullness of the collecting method of blood samples in filter paper strips for anti-dengue antibody detection using an ELISA Inhibition test which has been recently developed in our laboratory, blood samples collected on filter paper strips and serum samples were obtained from the same blood donors. Both samples were kept at -20ºC and tested at 15 days, 3 and 6 months. Titers were determined in positive samples. Comparing the results, no significant differences were found between serum samples and blood samples collected in filter paper strips in relation to the number of negative and positive cases. Nevertheless, it was observed in both types of samples a decrease of the antibody titers (one dilution in the longest period of our study (6 months.

  7. Application of an ELISA Milk Pregnancy Test in Beef Cows.

    Science.gov (United States)

    Roberts, J N; Byrem, T M; Grooms, D L

    2015-08-01

    Pregnancy-associated glycoproteins (PAG) are secreted by the binucleate giant cells of the ruminant placenta and enter maternal circulation at the time of placental attachment. The IDEXX Milk Pregnancy Test (IDEXX, Westbrook, ME) detects a subset of PAG in milk. Although designed as a management tool for dairy cows, there is potential for using the milk PAG test in beef cows. Our objective was to compare the performance of the milk PAG ELISA with a gold standard method for pregnancy diagnosis and determine the agreement between milk and serum PAG analysis in lactating beef cows. Angus and Angus-crossed cows (n = 332) from two Michigan beef herds were enrolled in this study. Cows were subjected either to timed artificial insemination followed by exposure to a bull or exclusively exposed to a bull. The bulls and cows were separated 30 days prior to examination. Serum and milk samples were collected and submitted within 24 h of collection to a commercial laboratory for PAG analysis using the IDEXX Milk Pregnancy Assay (milk) and the IDEXX Bovine Pregnancy Assay (serum). Concurrently with milk and serum collection, each cow was examined transrectally by palpation or ultrasonography. When compared to transrectal examination, the performance (and 95% confidence intervals) of the milk PAG ELISA was sensitivity of 99.7% (99.0-100.0%) and specificity of 80.8% (65.6-95.9%). The lower specificity is likely due to the low prevalence (9.9%) of open cows (n = 30) in the herds examined. Of the 332 cows examined, 1.8% (n = 6) were classified as rechecks using the milk PAG ELISA. Results of the milk and serum PAG ELISA were in high agreement (kappa coefficient = 0.91). The milk PAG ELISA was accurate in predicting pregnancy status using milk collected from beef cattle between days 37 and 125 post-insemination and may be useful for aiding management decisions in beef herds. PMID:26058919

  8. Estudio comparativo de un PCR anidado, ELISA y AGID en la detección del virus de la leucosis bovina en muestras de suero, sangre y leche Comparative study of nested PCR, ELISA and AGID tests in the detection of bovine leukaemia virus infection in serum, blood and milk samples

    Directory of Open Access Journals (Sweden)

    R Felmer

    2006-01-01

    Full Text Available Se evaluaron distintos métodos actualmente disponibles para el diagnóstico de la infección por el virus de la leucosis bovina (VLB. Los métodos empleados fueron AGID en suero, ELISA en muestras de suero y leche y PCR en linfocitos sanguíneos. De un total de 126 animales analizados, AGID identificó un menor número de animales positivos (75 comparado con las pruebas PCR y ELISA aplicadas en muestras de suero y leche (100. Tres animales positivos a AGID fueron negativos a PCR y 28 de las 51 muestras negativas a AGID fueron positivas mediante PCR. La sensibilidad diagnóstica de PCR con respecto a AGID fue de 96%, mientras que la especificidad fue de 45% (kappa 0,45. Todos los animales positivos a AGID fueron también positivos a ELISA aplicado tanto en suero como en leche, mientras que 25 animales negativos a AGID fueron consignados como positivos a ELISA, en ambas muestras biológicas. De esta forma, la sensibilidad diagnóstica de ELISA respecto a AGID fue de un 100%, mientras que la especificidad fue de 51% (kappa 0,55. La menor sensibilidad observada de AGID no es debido a reacciones falso positivas de ELISA y PCR, sino más bien a una mayor sensibilidad de estas últimas, lo que sugiere reconsiderar la utilización del método AGID en aquellos países en que aún se utiliza como método oficial en los programas de erradicación de leucosis.Different methods available for the detection of bovine leukaemia virus (BLV infection were evaluated. The methods evaluated were AGID in serum, ELISA in serum and milk, and PCR in blood lymphocytes. The AGID test identified a smaller number of positive animals (75/126 compared to PCR and ELISA tests (100/126. Three positive animals by AGID were negative by PCR and 28 of the 51 negative samples by AGID were positive by PCR. The sensitivity of PCR with respect to AGID was 96%, whereas the specificity was 45% (kappa 0.45. All positive animals by AGID were also positive by ELISA in serum and milk samples

  9. Modelizacion prospectiva del paisaje mediante sistemas de informacion geografica

    OpenAIRE

    Paegelow, Martin; Camacho Olmedo, Maria,; Menor Toribio, José

    2002-01-01

    Los Sistemas de Información Geográfica incluyen entre sus funciones de análisis aquellas destinadas a la modelización espacio-temporal. En este trabajo se presentan los resultados de la aplicación de un método de modelización que combina el análisis de una serie cronológica de usos del suelo (Cadenas de Markov), con la puesta en relación de estos usos y un conjunto de variables explicativas (Evaluación multicriterio), para, por último, asignar a cada píxel una categoría de ocupación del suelo...

  10. Comparison of DOT-ELISA and Standard-ELISA for Detection of the Vibrio cholerae Toxin in Culture Supernatants of Bacteria Isolated from Human and Environmental Samples.

    Science.gov (United States)

    Meza-Lucas, Antonio; Pérez-Villagómez, María-Fernanda; Martínez-López, José-Patricio; García-Rodea, Ricardo; Martínez-Castelán, María-Guadalupe; Escobar-Gutiérrez, Alejandro; de-la-Rosa-Arana, Jorge-Luis; Villanueva-Zamudio, Altagracia

    2016-09-01

    A comparison of DOT-ELISA and Standard-ELISA was made for detection of Vibrio cholerae toxin in culture supernatants of bacteria isolated from human and environmental samples. A total of 293 supernatants were tested in a double blind assay. A correlation of 100 % was obtained between both techniques. The cholera toxin was found in 20 Inaba and 3 Ogawa strains. Positive samples were from seafood (17 samples), potable water (1 sample) and sewage (5 samples). The DOT-ELISA was useful as the standard-ELISA to confirm the presence of cholera toxin in the environmental samples. PMID:27407304

  11. Individualismo y desconfianza en el trabajo asociativo de cadenas productivas de agroexportacion en el Valle del Mantaro, Junín, Perú

    Directory of Open Access Journals (Sweden)

    William Rodríguez Giráldez

    2014-12-01

    Full Text Available Objetivos: Determinar el individualismo y desconfianza en el trabajo asociativo de cadenas productivas de agroexportación en el Valle del Mantaro. Métodos: Investigación descriptiva, no experimental, de alcance transeccional correlacional. Fueron encuestados 383 productores agrícolas de las provincias que comprenden el Valle del Mantaro: Huancayo, Concepción, Jauja y Chupaca. Se utilizaron los estadísticos descriptivos, distribución de frecuencias, tablas de contingencia, y chi cuadrado para prueba de hipótesis. Resultados: En la dimensión individualismo: 72,23 % de productores manifestaron una actitud positiva hacia cadenas productivas; 87,2 % estimaron importante participar en reuniones; 88,5 % consideraron importante la capacitación en asociatividad y 86,2 % gustan trabajar conjuntamente con vecinos. Se deduce que el 83,53 % de ellos no tienen el rasgo de individualismo; por tanto, solo el 16,47 % tienen este rasgo y no influye negativamente en el trabajo asociativo en el Valle del Mantaro. En la dimensión desconfianza: 52 % de productores agrícolas manifestaron buena comunicación en la cadena productiva; 40 % aportaron entre 50 % y 100 % de costos de producción; 56 % podrían aportar entre 50 % y 100 % de costos de producción; 60 % señalaron que confían en empresas articuladoras y 60 % confían en colegas productores. Se deduce que, 53,6 % de estos productores no poseen el rasgo de desconfianza; mientras que 46,4 % si los tienen. Conclusiones: Solo 16,47 % de productores agrícolas tienen rasgos de individualismo y no influye negativamente en el trabajo asociativo; 46,4 % de estos productores tienen el rasgo de desconfianza y este valor sí influye negativamente en el trabajo asociativo en el valle.

  12. Competitividad del sistema agroalimentario de la cebolla de bulbo con enfoque de cadena productiva en el distrito de riego del alto chicamocha (boyacá)

    OpenAIRE

    Melo, Ligia Ines; Melo, María Mercedes; Rodríguez, Luis Felipe

    2010-01-01

    El análisis de la competitividad plantea una pregunta lógica sobre los determinantes que la originan, percibida ésta como un objeto móvil y, por tanto, que debe ser comprendida en términos de su dinámica. Por su importancia, el presente artículo se orienta al análisis de la competitividad del sistema agroalimentario de la cebolla de bulbo en el Distrito de riego del Alto Chicamocha (Boyacá), bajo el enfoque de cadena productiva, haciendo énfasis en los eslabones de producción y comercializaci...

  13. Evaluación de competencias de las regiones para el desarrollo de un clúster útil a la cadena productiva de pasifloras en Colombia

    OpenAIRE

    Leila N. Ramírez; Ángela M. Otálvaro

    2016-01-01

    En Colombia, el Ministerio de Agricultura y Desarrollo Rural (MADR) ha definido las cadenas productivas agrícolas con potencial para alcanzar la prosperidad regional y se encuentra impulsando iniciativas, entre las que está la creación de clústeres, con el fin de lograr ese objetivo. Esta investigación busca hacer explícita la metodología utilizada para definir las capacidades competitivas de las diferentes regiones productoras de pasifloras (frutas de carácter exótico que tiene un alto poten...

  14. Evaluación y relaciones entre las dimensiones del capital intelectual: El caso de la cadena de la madera de Oberá (Argentina)

    OpenAIRE

    Martos, Maria Susana; Fernández-Jardón Fernández, Carlos Mª; Figueroa Dorrego, Pedro

    2008-01-01

    Este trabajo investiga las tres dimensiones de capital intelectual, esto es, capital humano, capital estructural y capital relacional y sus interrelaciones. Para ello, se desarrolla un modelo que permite evaluar el capital intelectual en organizaciones ubicadas en zonas de países en vías de desarrollo. Se aplica a las empresas pertenecientes a la cadena de la madera del Departamento Oberá (Misiones). Los resultados obtenidos muestran que dentro del capital humano, lo más importante son las ac...

  15. Modelización de los indicadores de gestión en la cadena de suministro. una visión sistémica

    OpenAIRE

    MARTÍN DARIO ARANGO SERNA; GIOVANNY PEREZ ORTEGA; MIGUEL DAVID ROJAS LOPEZ

    2008-01-01

    El modelo que describe este artículo, pretende medir, analizar, evaluar y plantear un mejoramiento de los procesos administrativos, identificando las diferentes variables que intervienen en la gestión de la cadena de suministro centrándose en el subsistema de producción. Este trabajo hace parte de una investigación empírica que se viene desarrollando en el sector textil. El modelo muestra de forma empírica las relaciones que existen entre, las funciones internas de la empresa en el área de la...

  16. El papel de las TIC en el rendimiento de las cadenas de suministro: el caso de las grandes empresas de España y Portugal

    OpenAIRE

    Nicolás Gonzálvez- Gallego; Pedro Soto-Acosta; António Trigo; Francisco José Molina- Castillo; João Varajão

    2010-01-01

    Este artículo analiza las diferencias existentes entre las grandes empresas de España y Portugal en lo referido tanto al uso de las Tecnologías de la Información y la Comunicación (TIC) como a la integración electrónica con los agentes de la cadena de suministro. Estas diferencias se establecen en términos de la capacidad para generar resultados empresariales superiores. Los resultados muestran diferencias entre ambos grupos de empresas y confirman que las empresas españolas deberían persegui...

  17. Aproximación a la formación de precios en la cadena comercial de la pesca en Cataluña

    OpenAIRE

    García Antoni, Mónica

    2015-01-01

    El estudio nace con el propósito de describir y analizar la evolución de los precios a lo largo de la cadena comercial del pescado en Cataluña, España. Para ello se partió de recopilar los datos disponibles y, a partir de lo cual se ha puesto de relieve, la necesidad de una disponibilidad mayor de precios de productos de pescado fresco, para que fuese posible un análisis relevante. Dentro de esta situación, puesta en evidencia en el desarrollo del trabajo, el estudio ha quedado estructurado e...

  18. Análisis de variables estratégicas para la conformación de una cadena productiva de quinua en colombia

    OpenAIRE

    Montoya Restrepo, Luz Alexandra

    2006-01-01

    En este artículo se presenta una síntesis de la investigación realizada por el Grupo Interdisciplinario Biogestión sobre los usos y la producción de quinua en Colombia. Se ofrece un análisis estratégico del sector quinuero en el país a fin de identificar los factores clave de cambio que tienen un moderado o alto grado de gobernabilidad y que podrían afectar la conformación de la cadena productiva de quinua. También se proponen algunas estrategias orientadas a controlar estos factores de cambi...

  19. Estudio de factibilidad para el montaje de la cadena productiva agroindustrial de Productos Ancestrales, como parte del programa de productividad regional del departamento del Cauca

    OpenAIRE

    Amado Escobar, David Fernando

    2012-01-01

    En el presente trabajo monográfico, se diseño una cadena agroindustrial estándar para propiciar el desarrollo de productos alimenticios; a partir de la caracterización de los productos ancestrales más representativos de la cultura caucana. Para ello, se inicia con el estudio de las características geográficas, sociales y económicas que definen la situación del departamento frente a las dinámicas nacionales, precisando las líneas de acción que justifican la adopción de estrategias productivas....

  20. La cadena de suministro del sector metalúrgico: un análisis de sus principales eslabones (The Supply Chain in the metallurgical sector: an analysis of the main links

    Directory of Open Access Journals (Sweden)

    Patricia Carbajo-de-Lera

    2011-12-01

    Full Text Available Los directivos actuales conciben que la competencia ya no es empresa frente a empresa, sino que desborda este plano generándose una competencia cadena de suministro frente a cadena de suministro. Por ello, la gestión de la cadena de suministro se convierte en un elemento clave para la supervivencia empresarial y la obtención de ventajas competitivas. El diseño de la cadena de suministro viene definido, en buena parte, por las características del sector al que pertenece. En consecuencia, es de interés realizar análisis sectoriales. El presente trabajo tiene como objetivo analizar la cadena de suministro en un sector de gran importancia en la economía española, como es el metalúrgico. En concreto, buscamos determinar su cadena de suministro, definiendo los principales eslabones que la configuran y las relaciones entre ellos.Abstract: In the current business context, competition is no longer firm against firm, but has moved on to a higher plane, with the generation of supply chain against supply chain competition.  Therefore, Supply Chain Management (SCM has strategic relevance as a source of competitive advantage. The design of the supply chain is defined largely by the characteristics of the sector. Consequently, sectorial analysis is required. The aim of this paper is to analyze the supply chain of the metallurgical sector. Specifically, it will be focused on determining its supply chain and defining its main links and relationships.

  1. Consecuencias del efecto Bullwhip según distintas estrategias de gestión de la cadena de suministro: modelado y simulación = Bullwhip Effect Consequences according to Different Supply Chain Management Strategies: Modelling and Simulation

    Directory of Open Access Journals (Sweden)

    Campuzano Bolarín, Francisco

    2008-01-01

    Full Text Available Consecuencias del efecto Bullwhip según distintas estrategias de gestión de la cadena de suministro: modelado y simulación = Bullwhip Effect Consequences according to Different Supply Chain Management Strategies: Modelling and SimulationAbstract: El efecto Bullwhip es uno de los principales causantes de las inestabilidades en el proceso de gestión de demanda que se producen a lo largo de la cadena de suministro. El presente artículo expone un modelo capaz de recrear diferentes escenarios para la gestión de demanda en una cadena de suministro determinada, con independencia del número de niveles definidos en la cadena de suministro considerada. El modelo, realizado utilizando la metodología de la dinámica de sistemas, incorpora las variables necesarias para simular dicho proceso de gestión de demanda, como por ejemplo: niveles de inventario, órdenes de reabastecimiento, fabricación, previsiones u otras. Se muestra la utilidad del modelo propuesto, comparando los resultados que ofrecen dos escenarios diferentes, como son los representados por una cadena tradicional y el de una cadena reducida. = The Bullwhip effect is one of the main causes of instability in the management demand process along the Supply Chain. We introduce a model which is able to reproduce different Supply Chain Management Scenarios within a determinate Supply Chain with whichever the levels of this one. The model has been built using Systems Dynamics Methodology and incorporates the main variables which are required for simulating the Management Demand Process (Inventory levels, Replenishment orders, manufacturing process, forecasting etc.. This paper demonstrates the utility of the proposed model comparing the results offered by two different scenarios namely Traditional supply chain and Reduced supply chain.

  2. Leptospirosis serodiagnosis by ELISA based on recombinant outer membrane protein.

    Science.gov (United States)

    Chalayon, Piyanart; Chanket, Phanita; Boonchawalit, Toungporn; Chattanadee, Siriporn; Srimanote, Potjanee; Kalambaheti, Thareerat

    2011-05-01

    The outer membrane protein LipL21, LipL32, LipL41 and Loa22 of Leptospira interrogans serovar Copenhageni were previously revealed by immunoproteomic analysis, using sera from acute phase infection in a guinea pig. The full-length DNA of each protein was then cloned from the same serovar and expressed in pRSET vector. The obtained molecular weight (MW) of recombinant proteins rLipL21, rLipL32 and rLoa22 were slightly higher than the MW predicted from nucleotide sequences of each inserted gene, while only the N-terminal half of rLipL41 was obtained. Mice antiserum raised against each purified recombinant protein could react with the whole cell lysate of leptospiral serovars, implying that leptospiral native proteins shared a common epitope with recombinant protein. Serodiagnosis using recombinant protein antigen based on indirect ELISA procedure was developed in this study. The optimization of the ELISA components lead to determination of optical density (OD) from a single serum-dilution of 1:1000 in the leptospirosis patients group and normal healthy control group. The cut off OD values for both IgG and IgM class were investigated, and based on this fixed dilution only the IgG class could be used for differential diagnosis of patients and normal individuals. Compared with the MAT assay, ELISA assay utilizing both rLipL32 and rLoa22 as antigen, gave high accuracy and could thus be useful as a confirmative serology test. PMID:21353274

  3. Vaccination and ELISA: A partnership against Aujeszky's disease

    International Nuclear Information System (INIS)

    Aujeszky's disease is an important disease of swine. Vaccination is widely practised to control this disease. Although most vaccines offer clinical protection against the disease, they only reduce but do not prevent virus excretion or the establishment of latency after infection. Therefore vaccination alone will not lead to eradication of the disease. For this reason several serological test and elimination programmes have been developed. These programmes require either that pigs are not vaccinated or that they are vaccinated with vaccines that evoke an antibody response in serum that can be distinguished from the antibody response after infection. Differentiation between vaccinated and infected pigs is possible with the use of vaccine strains that lack one of the non-essential but highly immunogenic viral glycoproteins in combination with a glycoprotein specific antibody assay. Three such assays have been reported, based respectively on the demonstration of antibodies against the viral glycoproteins gI, gX and gIII. Because all field strains express gI and because most vaccines are gI negative, the presence of antibodies to gI seems the most logical indicator for infection with field virus. Several enzyme linked immunosorbent assay (ELISA) techniques have been developed to detect antibodies to gI, based either on the principle of a blocking ELISA or on the principle of an indirect ELISA, using affinity purified gI as antigen coated to the wells of microtitre plates. The antibody response against gI after infection of vaccinated and non-vaccinated pigs has been examined in detail. Infected pigs not vaccinated or vaccinated with a gI negative vaccine develop antibodies against gI which appear to persist for more than two years. Even pigs with maternal antibodies deficient in antibodies to gI that become infected with low doses of mildly virulent Aujeszky's disease virus develop antibodies to gI, albeit at low titres. (author). 51 refs, 1 fig

  4. Development of ELISA and immunochromatographic assay for ofloxacin

    Institute of Scientific and Technical Information of China (English)

    Wu Yong Sun; Wen Ying Liu; Ling Bo Qu

    2007-01-01

    Two rapid, sensitive and reliable immunoassay methods, namely competitive indirect enzyme-linked immunosorbent assay (CIELISA) and colloidal gold-based immunochromatographic assay (CGIA), were developed to detect ofloxacin (OFL). The linear range of the CI-ELISA was from 0.5 to 128 ng/mL with a limit of detection (LOD) of 0.35 ng/mL. Good recoveries were obtained in analyzing simulated swine urine samples. The CGIA could accurately estimate OFL at concentrations as low as 10 ng/mL in less than 10 min, and test results were read visually without any instrument.

  5. The ELisA Facility - RESTful API and Client Libraries

    CERN Document Server

    Corso-Radu, A; The ATLAS collaboration; Murillo Garcia, R

    2013-01-01

    The ATLAS experiment at the LHC (CERN) comprises a large and geographically distributed community of over three thousand scientists from all over the world. Data acquisition is supervised by a shift crew of about 10 people running the experiment 24/7. Information concerning the experiment operation, configuration and behavior has to be reported, gathered and shared with the whole community. To provide such functionality, a logbook facility tool, known as ELisA, has been developed to offer an user-friendly web interface to browse activity logs and an effective way for shifters and experts to report on system operations.\

  6. Rubella virus detection by ELISA method in exposed radiation workers

    International Nuclear Information System (INIS)

    Objective: A rapid diagnosis method was developed to detect Rubella virus infection in radiation workers. Methods: Modified ELISA method was used to detect the level of lgG and lgM antibodies in 514 in Jinan district. Results: 90.47% of 514 cases was shown to be resistant against Rubella virus; 6.42% were sensitive type; 0.78% belonged to be reinfected. Conclusion: Detection of Rubella virus in exposed radiation workers was imperative, and vaccine against Rubella virus was also needed to eliminate the infection risk. (authors)

  7. Consecuencias del efecto Bullwhip según distintas estrategias de gestión de la cadena de suministro: modelado y simulación = Bullwhip Effect Consequences according to Different Supply Chain Management Strategies: Modelling and Simulation

    OpenAIRE

    CAMPUZANO BOLARÍN, FRANCISCO; Lario Esteban, Francisco Cruz; Ros McDonnell, Lorenzo

    2008-01-01

    Consecuencias del efecto Bullwhip según distintas estrategias de gestión de la cadena de suministro: modelado y simulación = Bullwhip Effect Consequences according to Different Supply Chain Management Strategies: Modelling and SimulationAbstract: El efecto Bullwhip es uno de los principales causantes de las inestabilidades en el proceso de gestión de demanda que se producen a lo largo de la cadena de suministro. El presente artículo expone un modelo capaz de recrear diferentes escenarios para...

  8. Asunción del gobernador de facto de la provincia de Buenos Aires, general de división Oscar Bartolomé Gallino : Ceremonia transmitida por la cadena provincial de radios

    OpenAIRE

    Gallino, Oscar Bartolomé

    1981-01-01

    Por cadena provincial de radios, se transmite la ceremonia de asunción del segundo gobernador de facto de la provincia de Buenos Aires, Oscar Bartolomé Gallino. El locutor oficial menciona todas las emisoras integrantes de la cadena, se anuncian las autoridades presentes, se leen los antecedentes de Gallino, el decreto de su designación y el acta de la ceremonia. A continuación, jura Gallino ante los evangelios, por el Estatuto del Proceso de Reorganización Nacional, por la Constitución Nacio...

  9. Impacto del uso de las tecnologías de la información y la comunicación que apoyan la cadena de suministro (TIC-CS) sobre el desempeño organizacional

    OpenAIRE

    Víctor Andrés Peñates Montes

    2015-01-01

    Este trabajo, por medio de una revisión de la literatura relevante, pretende determinar y analizar la naturaleza y la forma que tienen los impactos sobre el desempeño organizacional generados por el uso de las tecnologías de la información y la comunicación que apoyan la cadena de suministro (TIC-CS). Primero se realiza un breve esbozo de los conceptos de desempeño y de cadena de suministro, luego se revisan y discuten los resultados de veintiséis investigaciones empíricas relacionadas con di...

  10. Adsorción de boro mediante perlas de alginato

    OpenAIRE

    Seira Ibáñez, Juana

    2008-01-01

    En este proyecto se propone la técnica de la adsorción mediante la utilización de polímeros naturales para eliminar el boro de residuos industriales, puesto que estos residuos presentan una gran problemática medioambiental. El polímero elegido para realizar la adsorción en este estudio es el alginato. Para poder trabajar en estado sólido se transforma el alginato de sodio, que es soluble en agua, en gel mediante la fabricación de las perlas de alginato de calcio. (Se utiliza...

  11. Desarrollo comunicación Alfa Arduino mediante Scada

    OpenAIRE

    Trallero Calvo, Jorge

    2015-01-01

    El siguiente proyecto pretende crear un sistema IoT (Internet of Things) girando en torno a Arduino, demostrando de este modo la facilidad de creación de proyectos y fiabilidad que nos aporta Arduino. Mediante una comunicación TCP/IP se procede a crear un sistema el cual es capaz de registrar valores a una cierta distancia mediante protocolos de procesos y comunicación (Telemedida) y de este modo monitorizar la temperatura en un punto determinado, sin estar limitado por la t...

  12. El fenómeno del Palo de Hockey de las cadenas de suministro en empresas manufactureras: un análisis mediante dinámica de sistemas y experimentos de laboratorio

    OpenAIRE

    Mora Luna, Luz Adriana

    2013-01-01

    Resumen: Cuando es difícil alcanzar la utilidad mensual esperada, los minoristas suelen establecer un sistema de oferta de descuentos para incrementar la demanda del mercado, generándose una acumulación de pedidos al final del reporte mensual de ventas. Esta situación se conoce como el fenómeno del Palo de Hockey. A pesar que la generación de este fenómeno se ha descrito en la literatura desde hace varios años, hay poca investigación que estudie las posibles causas que generan su comportamien...

  13. Comparative Evaluation of Fast Enzyme Linked Immunosorbent Assay (Fast-ELISA and Standard-ELISA For The Diagnosis Of Human Hydatidosis

    Directory of Open Access Journals (Sweden)

    MB Rokni

    2006-06-01

    Full Text Available Fast enzyme linked immunosorbent assay (Fast-ELISA was compared with the standard ELISA for the diagnosis of human hydatidosis. Seventy serum samples including 30 from hydatidosis patients (surgically confirmed, healthy control individuals not infected with any parasitic diseases (n=/20 and from others with different parasitic infections including, toxocariosis (n=5, fasciolosis (n=5, trichostrongylosis (n=5, and strongyloidosis (n=5 were analysed for anti-hydatid IgG antibodies using sheep hydatid cyst fluid antigen. The sensitivity, specificity, positive and negative predictive values, as well as validity of the test were found as 96.7%, 95.2%, 93.7%, 97.5% and 96% for conventional ELISA, while these paramters for fast-ELISA were respectively as follows: 100%, 97.5%, 96.7%, 100% and 98.8%. Regarding standard-ELISA 3µg/ml of antigen, serum dilution of 1:500, conjugate dilution of 1:3000 and 30 min incubation were found optimal, while for fast-ELISA 3µg/ml of antigen, serum dilution of 1:125, conjugate dilution of 1:1000 and 5 min incubation were utilized. The present study indicates that fast ELISA can easily be performed in place of the standard ELISA for the serodiagnosis of human hydatidosis with the advantage of minimising consumed time and manpower hours. Moreover, this test can be utilized in screening tests to diagnos human hydatidosis.

  14. Development of a microplate reader compatible microfluidic chip for ELISA.

    Science.gov (United States)

    Hou, Fenghua; Zhang, Qin; Yang, Jianping; Li, Xinchun; Yang, Xiujuan; Wang, Shuping; Cheng, Zhiyi

    2012-08-01

    We report a novel microfluidic device use for sandwich enzyme-linked immunoassay assay (ELISA). The related procedures including the introduction of reagents, dilution and distribution of samples, as well as immobilization of enzyme can be readily carried out on a poly (dimethylsiloxane) (PDMS) chip. Particularly, this microfluidic chip comprising of two distinct parallel units, and has an identical dimension as a conventional microtiter plate, which offers access to the directly quantitative detection by the microplate reader. Gradient-concentration reacting solutions at six different concentrations level generated by the microfluidic channel network are simultaneously transported to 24 reaction chambers to form enzymatic products. Alkaline phosphatase (ALP), 4-methylumbelliferyl phosphate (4-MUP) and KH(2)PO(4) are used as enzyme-substrate-inhibitor model, to demonstrate the utility of the developed microchip-based enzyme inhibitor assay. Various conditions such as the surface treatment of chip channels, fluids velocities, substrate concentration, and buffer pH are investigated. The present microfluidic device for ELISA holds several advantages, for instance frugal usage of samples and reagents, less of operating time, favorably integrated configuration, ease of manipulation, and could be explored to a variety of high throughput drug screening. PMID:22526682

  15. eLISA: Astrophysics and cosmology in the millihertz regime

    CERN Document Server

    Amaro-Seoane, Pau; Babak, Stanislav; Binétruy, Pierre; Berti, Emanuele; Bohé, Alejandro; Caprini, Chiara; Colpi, Monica; Cornish, Neil J; Danzmann, Karsten; Dufaux, Jean-François; Gair, Jonathan; Jennrich, Oliver; Jetzer, Philippe; Klein, Antoine; Lang, Ryan N; Lobo, Alberto; Littenberg, Tyson; McWilliams, Sean T; Nelemans, Gijs; Petiteau, Antoine; Porter, Edward K; Schutz, Bernard F; Sesana, Alberto; Stebbins, Robin; Sumner, Tim; Vallisneri, Michele; Vitale, Stefano; Volonteri, Marta; Ward, Henry

    2012-01-01

    This document introduces the exciting and fundamentally new science and astronomy that the European New Gravitational Wave Observatory (NGO) mission (derived from the previous LISA proposal) will deliver. The mission (which we will refer to by its informal name "eLISA") will survey for the first time the low-frequency gravitational wave band (about 0.1 mHz to 1 Hz), with sufficient sensitivity to detect interesting individual astrophysical sources out to z = 15. The eLISA mission will discover and study a variety of cosmic events and systems with high sensitivity: coalescences of massive black holes binaries, brought together by galaxy mergers; mergers of earlier, less-massive black holes during the epoch of hierarchical galaxy and black-hole growth; stellar-mass black holes and compact stars in orbits just skimming the horizons of massive black holes in galactic nuclei of the present era; extremely compact white dwarf binaries in our Galaxy, a rich source of information about binary evolution and about futur...

  16. Evaluación de competencias de las regiones para el desarrollo de un clúster útil a la cadena productiva de pasifloras en Colombia

    Directory of Open Access Journals (Sweden)

    Leila N. Ramírez

    2016-01-01

    Full Text Available En Colombia, el Ministerio de Agricultura y Desarrollo Rural (MADR ha definido las cadenas productivas agrícolas con potencial para alcanzar la prosperidad regional y se encuentra impulsando iniciativas, entre las que está la creación de clústeres, con el fin de lograr ese objetivo. Esta investigación busca hacer explícita la metodología utilizada para definir las capacidades competitivas de las diferentes regiones productoras de pasifloras (frutas de carácter exótico que tiene un alto potencial de desarrollo comercial. Se trata de establecer la mejor ubicación dentro de las regiones potenciales para el clúster en esta cadena en Colombia. Se emplea una matriz de evaluación basada en el diamante de competitividad de Porter. Esto permitió establecer que el Huila es la región que alcanza el mayor nivel competitivo para el desarrollo de este tipo de agrupaciones.

  17. Consolidación de carga como mecanismo de coordinación en cadenas de suministro de perecederos: Estudio de simulación

    Directory of Open Access Journals (Sweden)

    Juan Pablo Castrellón-Torres

    2015-01-01

    Full Text Available Una de las necesidades más apremiantes del sector primario es el desarrollo de métodos, herramie ntas, modelos y técnicas para u na gestión adecuada y eficiente de productos perecederos a lo largo de Cadenas de Suministro (SC, pa rticularmente enfocados en lo s servicios logísticos especializad os (ThirdParty Logistics 3PL como la consolid ación de carga. Esta estrategia de coordinación busca la alineación de los agentes y procesos para la eficiente manipulación de carga, minimi zando costos y permitiendo el uso adecuado de capacidades de medios de transporte. La cont ribución de este artículo se centra en el modelado y análisis de beneficios de las estrategias de consolidación de carga en el contexto de Cadenas de Suministro Agrícolas (ASC a través de la simulación. Los resultados de la simulación de eventos discretos, evaluando un producto commodity en tres diferentes escen arios, prueba los beneficios de esta e strategia de coordinación al reducir el nú mero anual de envíos de 1732 a 725 en un contex to de logística urbana e incrementando los nivel es de utilización de la flota hasta el 71%.

  18. SIMPLIFIED DIAGNOSIS OF MALARIA INFECTION: GFM/PCR/ELISA A SIMPLIFIED NUCLEIC ACID AMPLIFICATION TECHNIQUE BY PCR/ELISA

    Directory of Open Access Journals (Sweden)

    Ricardo Luiz Dantas MACHADO

    1998-09-01

    Full Text Available We report an adaptation of a technique for the blood sample collection (GFM as well as for the extraction and amplification of Plasmodium DNA for the diagnosis of malaria infection by the PCR/ELISA. The method of blood sample collection requires less expertise and saves both time and money, thus reducing the cost by more than half. The material is also suitable for genetic analysis in either fresh or stored specimens prepared by this method.Relatamos a adaptação de uma técnica para coleta de amostras (MFV e outra para extração, amplificação de DNA de parasitas da malária para diagnóstico por PCR/ELISA. O método de coleta de amostras requer menos habilidade e economisa tempo e dinheiro, assim reduzindo a mais da metade o custo. O material é também adequado para análise genética em especimens frescos ou estocados, preparados por este método.

  19. Massive Black Hole Science with eLISA

    CERN Document Server

    Barausse, Enrico; Berti, Emanuele; Holley-Bockelmann, Kelly; Farris, Brian; Sathyaprakash, Bangalore; Sesana, Alberto

    2014-01-01

    The evolving Laser Interferometer Space Antenna (eLISA) will revolutionize our understanding of the formation and evolution of massive black holes along cosmic history by probing massive black hole binaries in the $10^3-10^7$ solar mass range out to redshift $z\\gtrsim 10$. High signal-to-noise ratio detections of $\\sim 10-100$ binary coalescences per year will allow accurate measurements of the parameters of individual binaries (such as their masses, spins and luminosity distance), and a deep understanding of the underlying cosmic massive black hole parent population. This wealth of unprecedented information can lead to breakthroughs in many areas of physics, including astrophysics, cosmology and fundamental physics. We review the current status of the field, recent progress and future challenges.

  20. Evaluation of an indirect ELISA for detection and typing of foot-and-mouth disease virus

    International Nuclear Information System (INIS)

    An indirect enzyme linked immunosorbent assay (ELISA) kit was used for diagnosis of foot-and-mouth disease virus (FMDV) types O1, A23, C3 which occurred in Rio Grande do Sul State, Southern Brazil during 1984-1994. The samples were randomly selected and tested by ELISA, Complement Fixation Test (CFT) and in tissue culture. Out of 106 samples 78 (73,5%) were positive by ELISA and 39 (36,8%) were found positive in CFT, when original suspensions were used. Once these samples were inoculated onto tissue culture both tests gave similar results, although ELISA picked up more positive samples during the 1st passage in tissue culture. The negative samples (16) included in this study were negative in all tests. The ELISA was more sensitive than and as specific as CFT. ELISA and tissue culture together were shown to be a better system for detection of foot-and-mouth disease virus antigen than CFT. (author)

  1. Development and Comparative Evaluation of a Competitive ELISA with Rose Bengal Test and a Commercial Indirect ELISA for Serological Diagnosis of Brucellosis

    OpenAIRE

    Mythili, Tadepalli; Rajendra, Lingala; Bhavesh, Trangadia; Thiagarajan, Dorairajan; Srinivasan, Villuppanoor Alwar

    2011-01-01

    The development of a competitive ELISA for the detection of brucella-specific antibodies in bovines is described. Anti-brucella guinea pig serum was used as a source of competing antibodies. Lipo-polysaccharide purified from inactivated B. abortus S19 culture was used as antigen for the development of the assay. Sera from cattle were used in the competitive ELISA, rose bengal test and a commercial indirect ELISA. The following cattle sera were tested: (i) known positive sera (n = 80) (ii) kno...

  2. Reactivity in ELISA with DNA-loaded nucleosomes in patients with proliferative lupus nephritis.

    Science.gov (United States)

    Dieker, Jürgen; Schlumberger, Wolfgang; McHugh, Neil; Hamann, Philip; van der Vlag, Johan; Berden, Jo H

    2015-11-01

    Autoantibodies against nucleosomes are considered a hallmark of systemic lupus erythematosus (SLE). We compared in patients with proliferative lupus nephritis the diagnostic usefulness of a dsDNA-loaded nucleosome ELISA (anti-dsDNA-NcX) with ELISAs in which dsDNA or nucleosomes alone were coated. First, we analysed whether DNA loading on nucleosomes led to masking of epitopes by using defined monoclonal anti-DNA, anti-histone and nucleosome-specific autoantibodies to evaluate the accessibility of nucleosomal epitopes in the anti-dsDNA-NcX ELISA. Second, autoantibody levels were measured in these 3 ELISAs in 100 patients with proliferative lupus nephritis (LN) before immunosuppressive treatment and in 128 non-SLE disease controls. In patients with LN inter-assay comparisons and associations with clinical and serological parameters were analysed. The panel of monoclonal antibodies revealed that all epitopes were equally accessible in the anti-dsDNA-NcX ELISA as in the two other ELISAs. Patients with proliferative lupus nephritis were positive with dsDNA-loaded nucleosomes in 86%, with DNA in 66% and with nucleosomes in 85%. In the non-lupus disease control group these frequencies were 1.6% (2 out of 128) for both the anti-dsDNA-NcX and the anti-dsDNA ELISA and 0% in the anti-nucleosome ELISA. The levels in the anti-dsDNA-NcX ELISA were high in a group of patients with LN that showed absent reactivity in the anti-DNA or low levels in the anti-nucleosome ELISA. Anti-dsDNA-NcX positivity was associated with higher SLEDAI scores within this group. Within nucleosome-based ELISAs, we propose the anti-dsDNA-NcX ELISA as the preferred test system. PMID:26597199

  3. Double-antigen sandwich ELISA for the detection of anti-hepatitis C virus antibodies.

    Science.gov (United States)

    He, Jing; Xiu, Bingshui; Wang, Guohua; Chen, Kun; Feng, Xiaoyan; Song, Xiaoguo; Zhu, Cuixia; Ling, Shigan; Zhang, Heqiu

    2011-01-01

    A double-antigen sandwich ELISA was developed a detection of HCV antibodies by a recombinant multi-epitope HCV antigen and a biotin-streptavidin amplification system. Three plasma specimens from 1708 individuals who were suspected previously to be HCV-positive using an HCV antibody diagnostic kit (Chuangxin, Xiamen, China) displayed negative results when using the ELISA. These results were validated by a recombinant immunoblotting assay (two were negative, and one was indeterminate). Among 889 blood specimens donated for clinical evaluation, 246 were positive and 630 were negative using the ELISA. The sensitivity and specificity of the ELISA were 98.7% and 100%, respectively. In 43 donors and 14 patients with chronic hepatitis C, the detectable rates for HCV IgM by both ELISA and the HCV anti-IgM detection reagents (Huimin, Shenyang, China) were 100%, and the detectable rate for HCV IgG using an indirect HCV-antibody detection kit (GWK, Beijing, China) was 98.3%. Thus, the double-antigen sandwich ELISA exhibits strong specificity and sensitivity and has been approved by the China State Food and Drug Administration (SFDA). The performance of the double-antigen sandwich ELISA was similar to the Ortho ELISA 3.0. It did not give false-negative results otherwise IgM was undetectable using an indirect HCV-antibody detection kit. This ELISA provides another method for the detection of HCV antibodies. PMID:21029749

  4. CONSIDERACIONES SOBRE LA PLANIFICACIÓN DE PRODUCTOS ALIMENTICIOS EN UNA CADENA DE SUMINISTRO COMERCIAL / CONSIDERATIONS ON FOODSTUFF PLANNING IN A COMMERCIAL SUPPLY CHAIN

    Directory of Open Access Journals (Sweden)

    Neyfe Sablón-Cossío

    2013-09-01

    Full Text Available En la actualidad, la necesidad de pasar a la gestión de cadenas de suministro en la economía cubana se refleja a través de diversos síntomas que expresan la falta de coordinación entre las diversas redes de empresas. El presente trabajo muestra la aplicación de este enfoque a la cadena de suministro del puré de tomate, desde la base productiva nacional que abastece la industria, las importaciones de conservas y su abastecimiento a la red de mercados. Se realiza un análisis de la planificación colaborativa como concepto coordinador de planes conjuntos entre los actores claves en esta red. Como problemática principal se detectan: el desconocimiento de la demanda de los clientes, la inadecuada gestión de los productos en mercados locales y la baja integración de la cadena para el logro de alta disponibilidad. Como resultado se propone la planificación colaborativa como base de la filosofía de gestión para la mejora de la satisfacción del cliente.AbstractNowadays, it is necessary a change to the supply chain management in the Cuban economy, which is showed through many symptoms that express the lack of coordination in the enterprise networks. The present work applies this approach to the tomato paste commercial supply chain, from the national production and industry, the imports of this product and its supply to the network of markets. The collaborative planning is analysed as the main concept for the coordination in this supply chain. There were detected problems such as: the lack of knowledge about the demand, the inappropriate product management in local markets and the low integration of the supply chain in order to reach high levels of availability. As a result, it is proposed the collaborative planning as the basic philosophy in order to improve the client satisfaction.

  5. Simulación de Cadenas de Suministro: Nuevas Aplicaciones y Áreas de Desarrollo Supply Chain Simulation: New Applications and Development Areas

    Directory of Open Access Journals (Sweden)

    José L Calderón

    2007-01-01

    Full Text Available El presente trabajo es una revisión de la literatura sobre simulación de cadenas de suministro y resume los conceptos principales sobre simulación y analiza más de 70 artículos publicados entre los años 2000 y 2006. El objetivo es brindar una revisión crítica para quienes empiezan su investigación en esta área o están interesados en la simulación como herramienta para mejorar la cadena de suministro en los niveles estratégico, táctico y operativo. Selecciona 40 artículos en función de sus nuevos enfoques y aplicaciones, y los clasifica según ocho criterios: objetivos, tipo de análisis, etapas de la cadena de suministro abarcadas, procesos simulados, grado de detalle de cada eslabón, simulación del nivel estratégico, tipo de modelo de simulación, y simulación de modelos de evento discreto. El trabajo concluye determinando nueve áreas de desarrollo de los nuevos enfoques y aplicaciones, de las cuales los sistemas multi-agentes y las estrategias de colaboración y coordinación son los más empleados y estudiadasA literature review on supply chain simulation is presented. The paper summarizes the main concepts on simulation and analyzes more than 70 papers published between the years 2000 and 2006. The objective is to offer a state of the art for those who begin research in this area or are interested in the simulation as a tool to improve the supply chain in the strategic, tactical and operative levels. Fourty articles are selected considering the new approaches and applications, and classifies them according to eight criteria: objectives, analysis type, supply chain stages included, simulated processes, detail of each stage, simulation of the strategic level, type of simulation models, and simulation models of discrete events. The work finishes determining nine development areas of the new approaches and applications, of which the multi-agents systems and the strategies of collaboration and coordination are the most used

  6. Cadenas logísticas de exportación de frutas y desarrollo local en el sureste de México

    Directory of Open Access Journals (Sweden)

    Carlos Martner Peyrelongue

    2006-12-01

    Full Text Available La inserción del Sureste mexicano en el proceso de globalización ha sido difícil. La desaparición de actividades económicas, la exclusión y la migración han sido manifestaciones de su deterioro en la última década. No obstante, la región cuenta con algunos nodos o ámbitos locales específicos cuyas actividades y productos tienen potencial para insertarse en las redes de producción-distribución globalizadas. En este sentido, el presente artículo analiza el desarrollo de la cadena de exportación de una fruta tropical (la piña fresca bajo la hipótesis de que el impulso innovador y fortalecimiento de las redes productivas locales en territorios del sureste mexicano está estrechamente vinculado a la formación de cadenas logísticas, así como al control operativo, tecnológico y espacio-temporal que los actores locales alcanzan sobre las mismas. El planteamiento teórico se apoya en el enfoque sistémico de la cadena logística, que prescinde de las divisiones conceptuales tajantes entre producción, distribución y consumo, al poner énfasis en la circulación, es decir, en los flujos, cuya capacidad de movilización es fundamental para articular, integrar y dar viabilidad a los territorios en un contexto de relaciones globalizadasThe insertion of the Mexican southeast region into the globalization process has been a difficult task. The loss of economic activity, exclusion, and migration have been signs of the region's deterioration in the last decade. Nonetheless, this region contains several local"nodes" or spheres whose products and activities have the potential to participate in global production-distribution networks. This paper analyzes the export chain development of a tropical fruit (pineapple under the hypothesis that innovation support and strengthening of local production networks in southeast Mexico are closely linked to the formation of logistic supply chains and technical and operative control undertaken by local

  7. Control de velocidad mediante relación voltajefrecuencia

    OpenAIRE

    Alfonso Álzate; Duberney Murillo Yarce; Marcela González Valencia

    2011-01-01

    Se presenta la aplicación de un control digital utilizando un DSP, para controlar la velocidad de un motor de inducción trifásico en lazo cerrado mediante la técnica de control voltaje-frecuencia. También se presenta un panorama general de las técnicas1 de control escalar y vectorial, analizando en detalle la técnica escalar voltaje-frecuencia.

  8. Una descomposición de la regla de la cadena: un modelo cognitivo para la construcción del concepto

    OpenAIRE

    Valdivia, Cristóbal; Parraguez, Marcela

    2013-01-01

    El propósito del presente reporte es dar a conocer los resultados de la elaboración de una descomposición genética, sobre el concepto de la regla de la cadena, a partir de la Teoría APOE (Acción, Proceso, Objeto y Esquema) y la Tríada de Piaget y García (Piaget y García, 1989): Intra, Inter y Trans. La estructura general del estudio está basada en el ciclo de investigación que contiene la teoría: análisis teórico; diseño y aplicación de instrumentos; análisis y verificación de datos. En el se...

  9. Televisión en Europa: análisis y comparativa de las principales cadenas públicas en seis países

    OpenAIRE

    Camáñez García, Gemma Cristina

    2005-01-01

    En España se vive un momento en el que se va a implantar una nueva definición del servicio público de televisión, y en el que se estudia la adjudicación de nuevas licencias de emisión a cadenas de titularidad privada. El sector de la televisión se caracteriza por estar en constante evolución. La ley de la oferta y la demanda es la moneda de cambio de este medio, y obliga a una renovación inmediata en cuanto a contenidos y tecnologías aplicadas (internet, telefonía móvil, digital, ...

  10. Cadenas logísticas de exportación de frutas y desarrollo local en el sureste de México

    OpenAIRE

    Carlos Martner Peyrelongue

    2006-01-01

    La inserción del Sureste mexicano en el proceso de globalización ha sido difícil. La desaparición de actividades económicas, la exclusión y la migración han sido manifestaciones de su deterioro en la última década. No obstante, la región cuenta con algunos nodos o ámbitos locales específicos cuyas actividades y productos tienen potencial para insertarse en las redes de producción-distribución globalizadas. En este sentido, el presente artículo analiza el desarrollo de la cadena de exportación...

  11. Cadenas operativas y suelos de ocupación. El nivel 9 de la cueva de Las Caldas (Asturias, España

    Directory of Open Access Journals (Sweden)

    CORCHÓN, M.S., ORTEGA, P. y VICENTE, F.J.

    2013-01-01

    Full Text Available El amplio registro de puntas foliáceas procedentes de la Cueva de Las Caldas permite realizar un estudio pormenorizado de las cadenas operativas en el Solutrense superior. El objetivo perseguido es el conocimiento de las prácticas sociales que subyacen en la realización de estos útiles característicos por parte de los grupos de cazadores-recolectores solutrenses. Los procesos de talla implican la dispersión de desechos de talla característicos, que pueden ser reconocidos e identificados, como sucede en el nivel 9 de Las Caldas. Éste, identificado en la Sala I del yacimiento como un paleo-relieve con materiales in situ del Solutrense superior, constituye un escenario idóneo para este tipo de estudios.

  12. Comercialización on-line de cítricos ecológicos. Una alternativa viable a la cadena de distribución tradicional

    OpenAIRE

    FORTUNY CANO, MARCOS ANTONIO

    2014-01-01

    El presente trabajo gira en torno a la comercialización a través de Intenet, de los cítricos obtenidos en una explotación agrícola ecológica ubicada en la Región de Murcia. Los propietarios de dicha explotación fueron participes hasta hace poco tiempo de la clásica cadena de distribución de productos hortofrutícolas, vendiendo su producción a distintas cooperativas y mayoristas para ser posteriormente distribuida ésta a otros intermediarios. Dicha fórmula de comercialización ha mostrado siemp...

  13. Dinámica del empleo informal en Colombia: Una aproximación desde cadenas de Markov y funciones de riesgo. Primer trimestre de 2010.

    OpenAIRE

    Venegas Venegas, Benjamín Eduardo

    2012-01-01

    El presente documento presenta un análisis de la duración del empleo informal en Colombia a partir de la Gran Encuesta Integrada de Hogares sustentado en un análisis de funciones de riesgo y cadenas de Markov. Como principal resultado se destaca el hecho de que la informalidad en Colombia ha sido un fenómeno estable a través del tiempo y se espera que se consolide al correr de los años, además que son los más jóvenes y educados los que menos duran en la informalidad y que en té...

  14. DETECCIÓN DE UN DEFECTO GENÉTICO EN BOVINOS MEDIANTE UNA PRUEBA DE ADN. Detection of a bovine genetic defect by a DNA probe

    Directory of Open Access Journals (Sweden)

    Ricardo Felmer D.

    2001-01-01

    Full Text Available La deficiencia en la capacidad de unión de leucocitos bovinos a los antígenos, más conocida como BLAD, es una enfermedad hereditaria que resulta letal para el ganado de la raza Holstein. Su principal característica es ser una enfermedad autosómica recesiva que puede ser transmitida a la descendencia. El objetivo del trabajo fue estandarizar la metodología para realizar un diagnóstico de la enfermedad mediante técnicas moleculares y lograr una primera aproximación sobre la frecuencia génica del alelo mutado en algunas poblaciones de toros de la IX y X Región. En base a la amplificación del ADN mediante reacción en cadena de polimerasa (PCR y posterior digestión con enzimas de restricción Taq I y Hae III, fue posible visualizar, mediante electroforesis en gel de agarosa al 4%, los fragmentos de restricción característicos para bovinos normales, portadores o enfermos. La técnica se validó mediante un muestreo de 59 bovinos. De los 55 toros analizados uno resultó ser portador de BLAD, lo que implica una frecuencia génica de 1,79% en la población de toros de la IX y X Región. La técnica de PCR acoplada a la digestión con enzimas de restricción (Hae III y Taq I ha demostrado que identifica inequívocamente el genotipo del ganado en un locus determinado y permite el diagnóstico certero y precoz de aquellos animales enfermos y/o portadores del defecto hereditario conocido como BLAD.The adhesion deficiency of bovine leukocytes to antigens, known as BLAD (bovine leukocyte adhesion deficiency, is a hereditary genetic disease which is lethal for the Holstein breed. It is a recessive autosomal disease that can be transmitted to the offspring. The objective of this research was to standardize the molecular techniques to diagnose BLAD and also to get a first approximation of the genetic frequency of the mutated allele in the bull population of the IXth and Xth Regions. DNA amplification using polymerase chain reaction (PCR and

  15. Influencia de informalidad en titulación de tierras y planificación productiva, en cadenas agroexportadoras del Valle del Mantaro, Perú

    Directory of Open Access Journals (Sweden)

    William Rodríguez Giráldez

    2015-06-01

    Full Text Available El objetivo fue determinar la influencia de la informalidad en la titulación de tierras sobre la planificación productiva en cadenas agroexportadoras del Valle del Mantaro. Investigación de alcance descriptivo-correlacional, no experimental, transeccional. Fueron encuestados 383 productores agrícolas de las provincias que comprenden el Valle del Mantaro: Huancayo, Concepción, Jauja y Chupaca. Se utilizaron estadísticos descriptivos como, distribución de frecuencias, tablas de contingencia, y ji al cuadrado para prueba de hipótesis. Entre los resultados se tiene que, 1 En informalidad en titulación de tierras, 61 % de productores tienen título de propiedad y 2 % en trámite; 95,7 % saben de beneficios del título de propiedad; 55 % manifiestan que terrenos son de su propiedad; 45 % consideran que parte de sus terrenos los alquilan; 2 En planificación productiva, 52 % de productores deciden qué sembrar en función a la demanda y mejor precio de mercado, 62 % mejoraron resultados gracias a la planificación, 23 % lograron metas de producción entre el 75 % y 100 %, solo 19 % mejoraron su productividad esperada y 47 % mejoraron rangos inferiores a 25 %; además el 87 % consideran que en la planificación de la producción deben participar productores y empresas. En conclusión, participar en cadenas productivas no depende de la tenencia de terrenos con título de propiedad. El trabajo asociativo no depende de la decisión de sembrar, de la planificación, de mejoras de productividad, ni de participación en procesos de planificación.

  16. Development of a Novel PmpD-N ELISA for Chlamydia psittaci Infection

    Institute of Scientific and Technical Information of China (English)

    LIU Shan Shan; CHU Jun; ZHANG Qiang; SUN Wei; ZHANG Tian Yuan; HE Cheng

    2016-01-01

    ObjectiveChlamydia psittaci is an avian respiratory pathogen and zoonotic agent.The wide prevalence ofC. psittaci poses a threat to the poultry industry and its employees. However, few commercial kits are available for detecting avian antibodies excluding the in-house ELISA kit. In this study, we developed a novel ELISA kit for detecting antibodies againstC. psittaci based on the N-terminal fragment of polymorphic outer membrane protein D (PmpD-N) as the coating antigen. Methods The antigen concentrations, primary antibody, and cut-off value were determined and optimized. The ELISA, designated PmpD-N ELISA, was assessed for sensitivity, specificity, and concordance using sera samples from 48 experimentally infected and 168 uninfected SPF chickens. Results The sensitivity and specificity of PmpD-N ELISA were 97.9%, 100%, respectively, while the concordance was 98.1% as compared to that of MOMP-ELISA. No cross-reaction with positive sera for other avian pathogens was found. Using PmpD-N ELISA, 799/836 clinical samples were positive, including 93.0% and 98.1% positivity in layers and broilers, respectively. Conclusion These data indicate that indirect ELISA with PmpD-N as the antigen candidate is a promising approach for the surveillance ofC. psittaci infection.

  17. RT-PCR-ELISA as a tool for diagnosis of low-pathogenicity avian influenza

    DEFF Research Database (Denmark)

    Dybkær, Karen; Munch, Mette; Handberg, Kurt;

    2003-01-01

    low-pathogenicity AIV, from wild aquatic birds, and from domestic ducks. The AIV was detected in 32 swab pools by RT-PCR-ELISA compared to 23 by virus isolation (VI) in embryonated specific pathogen free (SPF) chicken eggs. Thus, 39% more specimens were positive by RT-PCR-ELISA than by VI. Two of the...

  18. An ELISA for the detection of antibodies against Newcastle disease virus in African village poultry

    International Nuclear Information System (INIS)

    A standardised and robust ELISA was developed for detecting antibodies against Newcastle disease in poultry. Results from 28 field samples were compared with the results from a haemagglutination test. It will be necessary to validate the ELISA in the field by assessing the results of serum samples collected from village chickens following vaccination or field infection. (author)

  19. Estimation of genetic parameters and transmitting ability for Minnesota Johne’s milk ELISA test

    Science.gov (United States)

    A total number of 45,907 results for Johne’s Milk ELISA test were received from Minnesota DHIA, of which 40,177 records were from herds with at least one positive Johne’s cow. Pedigree information was available for 19,304 Holstein cows from AIPL USDA representing 22,694 total records. Milk ELISA opt...

  20. Reactivity in ELISA with DNA-loaded nucleosomes in patients with proliferative lupus nephritis

    NARCIS (Netherlands)

    Dieker, J.W.; Schlumberger, W.; McHugh, N.; Hamann, P.; Vlag, J. van der; Berden, J.H.M.

    2015-01-01

    Autoantibodies against nucleosomes are considered a hallmark of systemic lupus erythematosus (SLE). We compared in patients with proliferative lupus nephritis the diagnostic usefulness of a dsDNA-loaded nucleosome ELISA (anti-dsDNA-NcX) with ELISAs in which dsDNA or nucleosomes alone were coated. Fi

  1. EMT nurjas Rate.ee ostuga Elisa kõnekaardiplaani / Gert D. Hankewitz

    Index Scriptorium Estoniae

    Hankewitz, Gert D.

    2006-01-01

    Ilmunud ka: Delovõje Vedomosti 12. apr. lk. 2. Elisa juhatuse esimehe Sami Seppäneni sõnul ostis EMT suhtlusportaali Rate.ee, kuna sai teada, et Elisa ning portaal plaanivad koos uue kõnekaardi turule tuua

  2. Elisa ja EMT kemplevad, kumb pakub paremat levi / Toivo Tänavsuu

    Index Scriptorium Estoniae

    Tänavsuu, Toivo

    2007-01-01

    Mobiilifirma Elisa väitel on Tartus kõige kvaliteetsem leviala Elisal. EMT võrgujuhi Margus Malvi hinnangul on EMT ja Elisa võrgud Tartus samaväärsed, ainus erinevus on kliendi kaugus tugijaamast. Lisa: Mobiililevi kvaliteet

  3. Immunodiagnosis of Fasciola hepatica infection in cattle using FAS2-ELISA

    International Nuclear Information System (INIS)

    Full text: Performance of Fas2-ELISA, ES-ELISA and coprology for the detection of Fasciola hepatica infection in cattle was evaluated in serum samples from 168 cattle killed in the abattoir of Lima Peru. In this population sample, 59 cattle had liver fluke infection determined by direct visualisation of flukes in the liver and by faecal inspection. The sensitivity, specificity, positive predictive value and negative predictive value of Fas2-ELISA were 96.6%, 93.5%, 89.6% and 98% respectively. Corresponding values for sensitivity, specificity, PPV and NPV for ES-ELISA were 94.9%, 83.4%, 75.6% and 96.8% and for faecal inspection were 89.8%, 100%, 100% and 94% respectively. No cross-reaction with sera from cattle infected with Moniezia sp. Bunostomum sp. and Dictyocaulus viviparus was observed with Fas2-ELISA as ES-ELISA cross-reacted to sera from Bunostomun sp. infected cattle (2/17). Fas2-ELISA is more sensitive and specific for the detection of F. hepatica infection in cattle than ES-ELISA and faecal inspection and might be useful as a reliable tool for the screening for bovine fasciolosis. (author)

  4. A COMPARATIVE STUDY OF A RAPID TEST AND ELISA FOR THE SEROLOGICAL DETERMINATION OF HIV INFECTION

    Directory of Open Access Journals (Sweden)

    Nemattolah Khansari

    1993-06-01

    Full Text Available In a comparative study, 460 serum samples were tested for the serological determination of human immunodeficiency vrius (HIV using an ELISA kit and a Rapid Test kit. Results indicated that the Rapid Test is reliable for screening unkonwn samples when there is no special equipment for screening with immunoblotting and/or ELISA.

  5. A COMPARATIVE STUDY OF A RAPID TEST AND ELISA FOR THE SEROLOGICAL DETERMINATION OF HIV INFECTION

    OpenAIRE

    Nemattolah Khansari; & Seyyed Ziaaddin Mazhari; & Hoori Rezvan

    1993-01-01

    In a comparative study, 460 serum samples were tested for the serological determination of human immunodeficiency vrius (HIV) using an ELISA kit and a Rapid Test kit. Results indicated that the Rapid Test is reliable for screening unkonwn samples when there is no special equipment for screening with immunoblotting and/or ELISA.

  6. Obtención de extractos de membrana externa de Vibrio cholerae O1, mediante el uso de diferentes detergentes

    Directory of Open Access Journals (Sweden)

    José Luis Pérez

    2006-04-01

    Full Text Available En la actualidad existen dos variantes principales de vacunas orales contra el cólera: una basada en células inactivadas de diferentes biotipos y serotipos y otra basada en la administración de cepas vivas genéticamente atenuadas. Una vacuna por subunidades pudiera ser una variante muy atractiva. Este trabajo describe la purificación parcial y caracterización preliminar de extractos de proteínas de membrana externa-lipopolisacárido (PME-LPS, obtenidos a partir de Vibrio cholerae O1, con el interés de seleccionar un proteoliposoma que posteriormente será estructurado en forma de cocleatos para su uso por vía oral en humanos. Las preparaciones fueron obtenidas a través del uso de diferentes detergentes. La cantidad de LPS en cada preparación fue estimada mediante la determinación de las unidades endotóxicas en el ensayo del Limulus (LAL. La composición de cada muestra fue evaluada mediante SDS-PAGE y Dot Blot. La inoculación intranasal (IN en ratones Balb/c se utilizó para la evaluación de la inmunogenicidad de las preparaciones, y la respuesta inmune fue determinada por ELISA y el título de anticuerpos vibriocidas. El tamaño molecular de la preparación con mejores resultados en inmunogenicidad se estimó mediante la cromatografía en Sephacryl S-1000. Se obtuvieron diferentes perfiles electroforéticos de acuerdo con el tipo de detergente utilizado. El LPS fue identificado en todas las preparaciones y aquella obtenida con el SDS al 15% mostró la más baja relación proteínas/LPS y los mejores resultados en los ensayos de inmunogenicidad. Adicionalmente se comprobó que su tamaño molecular es similar al observado en el proteoliposoma de VAMENGOC- BC. La preparación obtenida con el SDS al 15% constituye un proteoliposoma, con capacidad para estimular altos niveles de anticuerpos IgG anti-LPS y altos títulos de anticuerpos vibriocidas, luego de su administración por vía intranasal en ratones. Estos resultados constituyen

  7. Enrichment-ELISA for Detection of Salmonella typhi From Food and Water Samples

    Institute of Scientific and Technical Information of China (English)

    S.KUMAR; K.BALAKRISHNA; HV.BATRA

    2008-01-01

    Objective Development of monoclonal antibody based sandwich enzyme linked immunosorbant assay(sELISA)for rapid detection of Salmonella enterica serovar typhi(S.typhi) from food and water samples and optimization of enrichment procedures for use with the developed sELISA to increase the detection Sensitivity of the assay. Methods Spleen cells from BALB/c mice immunized with flagellin(H=d)antigen of S.typhi were fused with Sp2/0 myeloma cells.The hybridoma cell line specific to H=d antigen was established.characterized and ascites raised against one of these clones.The hypefimmune serum to flagellin antigen was raised in New Zealand White rabbits.An sELISA was developed using polyclonalantibody as capture and monoelonal antibody as detection antibody.To design the efficient culture sUrategies for use with the sELISA.different pre-enrichment and enrichment brothswere evaluated.The mediaincluded buffered peptonewater(BPW)and brain heart infusion broth for pre-enrichment and selenite F broth and Rappaport-Vassiliadis broth as enrichment broths.The developed sELISA with preceding enrichment step in BPW(Enrichment-ELISA)was evaluated in various food samples artificially inoculated with S. typhi bacteria.Various food(30)and water(35)samples collected from field were also tested by Enrichment-ELISA and culture method. Results Out of four specific clones to H=d antigen,one clone(#2/56.IgG2a isotype)was usedin sELISA.The sELISA had the detectionlimit of 104-105 cfu of S.typhi.Of the various broths used with sELISA,BPW was found to yield maximum ELISA values.Enrichment-ELISA,when tested in artificially inoculated food samples,generally,could detect 102S.typhi cfu/mL within 10 h from variousfood rinses(meat,vegetable)and milk samples.After overnight enrichment in BPW,as less as 2 bacteria per 10 mL of milk,meat rinse.and chicken rinse could be detected.Only one of the field samples(water)gave false positive result by Enrichment-ELISA.Conclusion In comparison to culture

  8. eLISA eccentricity measurements as tracers of binary black hole formation

    CERN Document Server

    Nishizawa, Atsushi; Klein, Antoine; Sesana, Alberto

    2016-01-01

    Up to hundreds of black hole binaries individually resolvable by eLISA will coalesce in the Advanced LIGO/Virgo band within ten years, allowing for multi-band gravitational wave observations. Binaries formed via dynamical interactions in dense star clusters are expected to have eccentricities $e_0\\sim 10^{-3}$-$10^{-1}$ at the frequencies $f_0=10^{-2}$ Hz where eLISA is most sensitive, while binaries formed in the field should have negligible eccentricity in both frequency bands. We estimate that eLISA should always be able to detect a nonzero $e_0$ whenever $e_0\\gtrsim 10^{-2}$; if $e_0\\sim 10^{-3}$, eLISA should detect nonzero eccentricity for a fraction $\\sim 90\\%$ ($\\sim 25\\%$) of binaries when the observation time is $T_{\\rm obs}=5$ ($2$) years, respectively. Therefore eLISA observations of BH binaries have the potential to distinguish between field and cluster formation scenarios.

  9. The use of ELISA to detect antibodies to Brucella abortus in naturally infected cattle

    International Nuclear Information System (INIS)

    An indirect ELISA using the immunodominant antigen, smooth lipopolysaccharide (SLPS) of Brucella abortus, was used to detect antibodies in a statistically selected dairy cattle population from the Sabana of Bogota (Colombia). Based on the FAO/IAEA Brucella ELISA kit, the test was standardized in terms of plates, reagents and reference sera. Quality control for routine testing was also established. Calculation of the appropriate cut-off optical density (O.D) value for negativity was made after discriminant analysis and examination of the frequency of the sera distributed as positive or negative. The sensitivity and specificity of the ELISA for diagnosis of Brucella infection were compared to the complement fixation (CF) test results and were 94.8% and 96.0% respectively. The use of ELISA for monitoring bovine populations undergoing an eradication of brucellosis is recommended. The application of an ELISA using B. bovis outer membrane proteins (OMP) as antigen is discussed. (author). 38 refs, 3 figs, 4 tabs

  10. Enzyme linked immunosorbent assay (ELISA) used for the detection of Cysticercus bovis injection in cattle

    International Nuclear Information System (INIS)

    Sensitivity and specificity of the ELISA for the diagnosis of T. saginata cysticercosis were studied. The specificity of the ELISA, by using ThFAS-L antigen, was found to be quite high when the hyperimmune serum samples of animals contained relatively high numbers of metacestodes. There was a direct relationship between the intensity of infection and the ELISA values of hyperimmune serum samples. Although ThFAS-L did not cross-react with F. hepatica and E. granulosus and gave no false positive reactions, it is not possible to regard this test as sensitive enough to detect naturally and weakly infected animals. Sensitivity and specificity of the ELISA may be improved by elution of the protein of MW 21800, which showed a diagnostic significance during immunoblot analysis. At present, the ELISA using ThFAS-L antigen has limited use in the diagnosis of cysticercosis infections in cattle

  11. Cystatin capture elisa immunodiagnosis of human fasciolosis at Chupaca-Junin Province

    International Nuclear Information System (INIS)

    To determine the prevalence of human fasciolosis in an endemic area by means of an Enzyme-Linked Immunosorbent Assay (ELISA) using cystatin as a capture agent for the detection of specific antibodies to fasciola hepatica cysteine proteinases. An ELISA plate was sensitized with cystatin, incubated with excretory-secretory products of adult flukes, and followed by standard ELISA procedures. Clinical applicability of the cystatin capture ELISA for the immunodiagnosis of fasciolosis was tested with 200 serum samples of children and adults from an endemic area in Chupaca province, Junin department. Serum samples from the endemic area tested by cystatin capture ELISA showed 27/200 (13,5%) of positive cases. Fasciolosis remains a major health problem at Chupaca province, Junin department

  12. Evaluation of ELISA and Brucellacapt Tests for Diagnosis of Human Brucellosis

    Directory of Open Access Journals (Sweden)

    Mohammad Ghasem Golmohammadi

    2013-03-01

    Full Text Available Background: Because of the difficulty in the diagnosis of brucellosis, particularly in endemic areas, the use of new and feasible diagnostic tests seem to be of great importance for resolving the diagnostic obstacles. We evaluated the usefulness of a new serological test based on an immunocapture-agglutination technique in comparison with ELISA test for serological diagnosis of brucellosis.Methods: A total of 11 patients with brucellosis, who had positive blood cultures for Brucella species, and 47 suspected patients were included in this study. Serum samples collected from these patients were tested by brucellacapt and ELISA and the results were, consequently, compared.Results: In patients with positive blood culture, all the samples gave positive results with brucellacapt test while IgM ELISA, IgG ELISA and (IgG + IgM ELISA tests were positive in 8, 9 and 11 patients, respectively. Out of the 46 suspected patients, (IgG + IgM ELISA, Brucellacapt, IgG ELISA and IgM ELISA were positive in 37, 15, 34 and 37 patients, respectively.The best cut-off point of ELISA-IgG was 10.78 IU/ml which produced the maximal sensitivity and specificity for the diagnosis of human brucellosis.Conclusion: Both the (IgG + IgM ELISA and Brucellacapt tests demonstrate a high specificity in this study. According to the results of the current study, it is found that both tests are valuable tools for diagnosis of brucellosis in Iran as an endemic area of brucellosis. It is strongly suggested that a combination of both tests to be used for the diagnosis of brucellosis

  13. Methods in virus diagnostics: from ELISA to next generation sequencing.

    Science.gov (United States)

    Boonham, Neil; Kreuze, Jan; Winter, Stephan; van der Vlugt, René; Bergervoet, Jan; Tomlinson, Jenny; Mumford, Rick

    2014-06-24

    Despite the seemingly continuous development of newer and ever more elaborate methods for detecting and identifying viruses, very few of these new methods get adopted for routine use in testing laboratories, often despite the many and varied claimed advantages they possess. To understand why the rate of uptake of new technologies is so low, requires a strong understanding of what makes a good routine diagnostic tool to begin. This can be done by looking at the two most successfully established plant virus detection methods: enzyme-linked immunosorbant assay (ELISA) and more recently introduced real-time polymerase chain reaction (PCR). By examining the characteristics of this pair of technologies, it becomes clear that they share many benefits, such as an industry standard format and high levels of repeatability and reproducibility. These combine to make methods that are accessible to testing labs, which are easy to establish and robust in their use, even with new and inexperienced users. Hence, to ensure the establishment of new techniques it is necessary to not only provide benefits not found with ELISA or real-time PCR, but also to provide a platform that is easy to establish and use. In plant virus diagnostics, recent developments can be clustered into three core areas: (1) techniques that can be performed in the field or resource poor locations (e.g., loop-mediated isothermal amplification LAMP); (2) multiplex methods that are able to detect many viruses in a single test (e.g., Luminex bead arrays); and (3) methods suited to virus discovery (e.g., next generation sequencing, NGS). Field based methods are not new, with Lateral Flow Devices (LFDs) for the detection being available for a number of years now. However, the widespread uptake of this technology remains poor. LAMP does offer significant advantages over LFDs, in terms of sensitivity and generic application, but still faces challenges in terms of establishment. It is likely that the main barrier to the

  14. Sensibilidad del cultivo de pool fecal para detectar infección por Mycobacterium avium subsp. paratuberculosis en rebaños bovinos de leche y su relación con la prueba de ELISA Sensitivity of pooled faecal culture for detecting Mycobacterium avium subsp. paratuberculosis infection in dairy herds and its relationship with the ELISA test

    Directory of Open Access Journals (Sweden)

    M Pradenas

    2008-01-01

    Full Text Available El objetivo del presente estudio fue evaluar la sensibilidad y especificidad del cultivo de pool fecal como alternativa diagnóstica de paratuberculosis en rebaños lecheros bovinos comparado con el cultivo fecal individual y la prueba de ELISA con la finalidad de reducir costos asociados al diagnóstico de la enfermedad. En 12 rebaños lecheros con antecedentes de paratuberculosis se recolectó un máximo de 50 muestras fecales individuales por rebaño (n=598 y se cultivaron en medio de Herrold en forma individual y en pools estratégicos de 5 y 10 animales. Simultáneamente, de cada animal se recolectó una muestra de sangre para la prueba de ELISA. Se evaluó la sensibilidad y especificidad de las pruebas usando como referencia los cultivos fecales individuales mediante tablas de dos entradas. En 10 (83,3% rebaños y en 42 (7% animales fue posible aislar Map en tanto que el 15,8% y 22% de los pools de 5 y 10 animales, respectivamente, resultaron positivos al cultivo fecal. La sensibilidad de los pools fecales de 5 y 10 animales fue 43,2% y 46,4%, respectivamente, mientras que la sensibilidad del ELISA fue 42,9%. El costo de los pools fecales de 5 animales fue similar al de la prueba de ELISA, pero cuatro veces menor que el costo del cultivo fecal individual; sin embargo, la sensibilidad y especificidad de los pools de 5 animales fue similar a los cultivos individuales. El cultivo de pools fecales demostró ser una buena y costo-efectiva alternativa para el diagnóstico de paratuberculosis para detectar rebaños infectados con MapThe aim of the present study was to evaluate the sensitivity and specificity of pooled faecal culture as a better alternative for the diagnosis of paratuberculosis in dairy herds compared to the conventional faecal culture and the ELISA test. Individual faecal and blood samples were collected from 50 cows in each one of 12 dairy herds (n = 598 with a history of paratuberculosis. Faecal samples were cultured on Herrold

  15. The FAO/IAEA ELISA kit quality assurance programme

    International Nuclear Information System (INIS)

    A system for quality assurance was first introduced into the rinderpest sero-monitoring network in 1989 and was designed to ascertain how well the FAO/IAEA rinderpest ELISA kit was functioning in individual laboratories. Of the 15 laboratories actively using the kit at that time, 9 submitted results. These showed that the kit was working well although the overall binding ratio (an indication of the ability of the assay to separate positive from negative sera) was sub-optimal. The results also revealed that in every laboratory the local negative population gave a lower value than the kit reference negative sera. This was not unexpected, as the kit reference negative serum was never intended to be used for the calculation of negative/positive cut-off values and was included only as a reference. The examination of local negative sera and the establishment of the mean local population negative value is essential to the establishment of the test in any laboratory. An examination of individual laboratory results indicated that where problems were encountered it was invariably due to the use of poor water for reconstitution of reagents. This has confirmed the need to supply sterile de-ionized water for re-constitution of all kit reagents. The results from this quality assurance exercise proved vital in identifying problems with the kit itself and with the use of the kit in individual laboratories. In the most part these problems have now been resolved. However, in retrospect it is considered that the approach adopted was over-complicated and in future quality assurance will involve the testing of a panel of 44 sera distributed to all participating laboratories once a year. (author). 6 figs, 2 tabs

  16. Development and comparative evaluation of a competitive ELISA with rose bengal test and a commercial indirect ELISA for serological diagnosis of brucellosis.

    Science.gov (United States)

    Mythili, Tadepalli; Rajendra, Lingala; Bhavesh, Trangadia; Thiagarajan, Dorairajan; Srinivasan, Villuppanoor Alwar

    2011-10-01

    The development of a competitive ELISA for the detection of brucella-specific antibodies in bovines is described. Anti-brucella guinea pig serum was used as a source of competing antibodies. Lipo-polysaccharide purified from inactivated B. abortus S19 culture was used as antigen for the development of the assay. Sera from cattle were used in the competitive ELISA, rose bengal test and a commercial indirect ELISA. The following cattle sera were tested: (i) known positive sera (n = 80) (ii) known negative sera (n = 100) and (iii) field sera (n = 1184). Based on the receiver operating characteristics curve analysis and frequency distribution of the percentage of inhibition, 30% inhibition was considered the cut-off for positive and negative results. The sensitivity and specificity estimate on comparison with the commercial indirect ELISA was 94.87 and 92.12% respectively. The competitive ELISA described is a simple method for the routine screening of animal sera for detecting Brucella-specific antibodies. PMID:23024418

  17. Comparison of ELISA and TLC Methods for the Morphine Detection in Urine of Drug Abusers

    Directory of Open Access Journals (Sweden)

    Alireza Timcheh-Hariri

    2016-04-01

    Full Text Available Background: The current study was conducted to compare ELISA with thin layer chromatography (TLC methods for diagnosis of morphine in the urine. Methods: Positive urine samples for morphine confirmed by immunochromatographic strips were collected from the Imam Reza Toxicology Laboratory, Mashhad University of Medical Sciences, Mashhad, Iran in 2012 for the current study. Then, the collated urine samples (70 were analyzed by both ELISA and TLC methods. Results: On analyzing samples by TLC, 57 out of 70 (81.4% revealed morphine spot, whereas by ELISA method all samples were positive. The difference was statisticaly significant (P=0.0001. Both immunoassays had the same 100% positive results. The possible 18.6% false positive results might be due to drug interactions. TLC is more specific but time-consuming and less sensitive than ELISA is. However, TLC is an old method but more reliable than ELISA. Conclusion: Contrary to the claim that commercially available ELISA kits have a high specificity for detection of morphine derivatives; it seems that false positive results may occur. It is thus recommended that all positive results obtained from ELISA be checked by a cheap widely available confirmation test of TLC or ideally by a quantitative technique such as GC-Mass spectroscopy, particularly for legal purposes.

  18. Tratamiento del síndrome subacromial mediante acromioplastia abierta

    OpenAIRE

    Arenas Planelles, Antonio; Garbayo Marturet, Antonio Jesús; Ayala Palacios, Higinio; Arenas Miquélez, A.

    2003-01-01

    Se presenta una serie de 161 casos de síndrome subacromial tratados quirúrgicamente en nuestro Servicio mediante acromioplastia abierta y reconstrucción del manguito en los casos en que existía ruptura del mismo. El estudio se ha realizado de forma retrospectiva, evaluándose una serie de variables clínicas, radiológicas y quirúrgicas, y algunos parámetros para valorar los resultados. Tras la intervención, con un tiempo de seguimiento medio de 45 meses, el 82% de los pacientes se encontraban s...

  19. RECURSOS NO CONVENCIONALES SUSCEPTIBLES DE SER EXPLOTADOS MEDIANTE FRACKING

    OpenAIRE

    Jódar Abellán, Antonio

    2014-01-01

    En el presente trabajo se analizan en detalle los recursos de origen no convencional existentes en el contexto internacional, europeo, nacional y regional, que pueden ser explotados mediante fracking. Así mismo, se mencionará someramente la legislación aplicable a la fracturación hidráulica y los impactos, tanto beneficiosos como perjudiciales, que ésta puede acarrear. El estudio finaliza con una propuesta de posibles lugares en la Península Ibérica donde dicha técnica reporte mayores benefic...

  20. Verificando diseños BON mediante Alloy

    OpenAIRE

    Castro, Pablo Francisco; Ponzio, Pablo Daniel; Demasi, Ramiro Adrián; Baum, Gabriel Alfredo

    2005-01-01

    En este artículo presentamos una técnica para traducir diseños estructurales expresados en el lenguaje BON, al lenguaje formal Alloy. En donde, la principal ventaja de la traducción es que puede realizarse automáticamente mediante herramientas de software. Adicionalmente, esta metodología puede ser usada para validar propiedades sobre los diseños utilizando el Alloy Analyzer. Para finalizar, mostramos la aplicación a un caso de estudio de Darwin Tool, una herramienta que implem...

  1. Control domótico remoto de vivienda mediante smartphone

    OpenAIRE

    ALEIXANDRE TUDO, BERNARDO

    2013-01-01

    La tecnología forma parte de la sociedad actual y de la vida cotidiana de las personas. Los últimos avances tecnológicos han simplificado nuestra vida y han provocado muchos cambios en las diversas áreas de la sociedad. Una de estas áreas es sin duda la automatización de la vivienda. Los nuevos aparatos electrónicos nos permiten tener acceso a las comunicaciones en cualquier lugar y a cualquier hora. La entrada de internet móvil en el mercado mediante los Smartphone y su ráp...

  2. Graphical control and evaluation of the operational performance of ELISA method for detection of trypanosomal antibodies

    International Nuclear Information System (INIS)

    The performance of four indirect trypanosomal antibody detection enzyme-linked immunosorbent assays (I-TAB ELISA's), exploiting native and denatured antigens of Trypanosoma congolense (T.c.AGn, T.c.AGd) and T. vivax (T.v.AGn, T.v.AGd), has been evaluated in fifteen laboratories from Africa and Europe. Standardised internal quality control samples (IQCs) were used as indicators and data plotted on charts to monitor and control the ELISA's at individual laboratories. Based on the overall data, dispersion of true values from the population data range was estimated plotting the location and deviation of raw and normalised absorbance values of the IQCs. Binding ratios were calculated to estimate the assay proficiency with respect to the accuracy of assessing that the QC samples tested positive or negative in the test proper. The frequency distribution of coefficients of variation <10% of IQCs was monitored. The use of this standardised and transparent IQC data charting provides a useful quality control tool for the evaluation of the performance of the ELISA's used for trypanosomosis serology. The method provides a measure of confidence in estimating the proficiency of national laboratories with respect to reported ELISA results on disease occurrence. Moreover, data were compiled of the inter-laboratory ELISA performance by means of summary data charts with reference to the performance criteria described. The data were also analysed using modified Youden plots. The analysis demonstrated similar laboratory proficiency for the I-TAB ELISA (T.c.AGn) (four of five laboratories), I-TAB ELISA (T.c.AGd) (eleven of fourteen laboratories), I-TAB ELISA (T.v.AGn) (three of five laboratories), and I-TAB ELISA (T.v.AGd) (eleven of fifteen laboratories). The impact of data variation between test and specified values as a sole decision criterion for acceptance or rejection of test plates is discussed. (author)

  3. Interpreting dual ELISA and qPCR data for bacterial kidney disease of salmonids.

    Science.gov (United States)

    Nance, Shelly L; Riederer, Michael; Zubkowski, Tyler; Trudel, Marc; Rhodes, Linda D

    2010-09-01

    Although there are a variety of methods available for the detection of Renibacterium salmoninarum, the causative agent of bacterial kidney disease in salmon and trout, the enzyme-linked immunosorbent assay (ELISA) is probably the most widely used method. However, ELISA measures bacterial antigen, which does not necessarily reflect the number of cells present. We hypothesized that dual analysis of kidney tissue by ELISA and a quantitative real-time polymerase chain reaction assay (qPCR) would provide complementary information about antigen level and the number of bacterial genomes. We found that DNA extracted from the insoluble fraction of the ELISA tissue preparation produced the same qPCR result as DNA extracted directly from frozen tissue, permitting true dual analysis of the same tissue sample. We examined kidney tissue in this manner from individual free-ranging juvenile Chinook salmon and antibiotic-treated captive subadult Chinook salmon and observed 3 different patterns of results. Among the majority of fish, there was a strong correlation between the ELISA value and the qPCR value. However, subsets of fish exhibited either low ELISA values with elevated qPCR values or higher ELISA values with very low qPCR values. These observations suggest a conceptual model that allows inferences about the state of infection of individual fish based on dual ELISA/qPCR results. Although this model requires further assessment through experimental infections and treatments, it may have utility in broodstock selection programs that currently apply egg-culling practices based on ELISA alone. PMID:21387990

  4. Quantification of Hordeins by ELISA: the correct standard makes a magnitude of difference.

    Directory of Open Access Journals (Sweden)

    Gregory J Tanner

    Full Text Available BACKGROUND: Coeliacs require a life-long gluten-free diet supported by accurate measurement of gluten (hordein in gluten-free food. The gluten-free food industry, with a value in excess of $6 billion in 2011, currently depends on two ELISA protocols calibrated against standards that may not be representative of the sample being assayed. AIM: The factors affecting the accuracy of ELISA analysis of hordeins in beer were examined. RESULTS: A simple alcohol-dithiothreitol extraction protocol successfully extracts the majority of hordeins from barley flour and malt. Primary hordein standards were purified by FPLC. ELISA detected different classes of purified hordeins with vastly different sensitivity. The dissociation constant (Kd for a given ELISA reaction with different hordeins varied by three orders of magnitude. The Kd of the same hordein determined by ELISA using different antibodies varied by up to two orders of magnitude. The choice of either ELISA kit or hordein standard may bias the results and confound interpretation. CONCLUSIONS: Accurate determination of hordein requires that the hordein standard used to calibrate the ELISA reaction be identical in composition to the hordeins present in the test substance. In practice it is not feasible to isolate a representative hordein standard from each test food. We suggest that mass spectrometry is more reliable than ELISA, as ELISA enumerates only the concentration of particular amino-acid epitopes which may vary between different hordeins and may not be related to the absolute hordein concentration. MS quantification is undertaken using peptides that are specific and unique enabling the quantification of individual hordein isoforms.

  5. El frágil inicio de la cadena de frío vacunal en España The fragile beginning of the vaccine cold chain in Spain

    OpenAIRE

    José Tuells

    2010-01-01

    La realización de dos estudios de ámbito nacional durante 1988-89 con el objetivo de analizar la situación de la cadena de frío vacunal en España activó el interés por la logística de las vacunas entre los profesionales de la salud pública. Se realizaron utilizando la metodología de evaluación basada en tarjetas de monitorización tiempo-temperatura (informe Battersby) y encuestas transversales sobre el estado de la cadena de frío vacunal (informe Ferrando) en los niveles provincial y local (2...

  6. Impacto del uso de las tecnologías de la información y la comunicación que apoyan la cadena de suministro (TIC-CS sobre el desempeño organizacional

    Directory of Open Access Journals (Sweden)

    Víctor Andrés Peñates Montes

    2015-10-01

    Full Text Available Este trabajo, por medio de una revisión de la literatura relevante, pretende determinar y analizar la naturaleza y la forma que tienen los impactos sobre el desempeño organizacional generados por el uso de las tecnologías de la información y la comunicación que apoyan la cadena de suministro (TIC-CS. Primero se realiza un breve esbozo de los conceptos de desempeño y de cadena de suministro, luego se revisan y discuten los resultados de veintiséis investigaciones empíricas relacionadas con dicho impacto. Se identifica que estas investigaciones reportan impactos de naturaleza positiva en cuatro formas diferentes. Se sugiere, finalmente, actuar usando complementariedades para disminuir los riesgos asociados a la eficacia del proceso de implementación de las TIC-CS.

  7. Prevalence of entamoeba histolytica and entamoeba dispar by means of microscopy and ELISA in a suburb of Lima

    International Nuclear Information System (INIS)

    To assess the prevalence of E. histolytica and E. dispar in a marginal population of Callao, Lima, using ELISAs. Two ELISAs were used, one of them for both Entamoeba species (Entamoeba-ELISA), and an E. histolytica-specific ELISA. 128 stool samples from randomized Callao inhabitants were microscopically examined. Thirteen (10%) were microscopically diagnosed as having E. histolytica or E. dispar infection; and 7 (6%) were positive to Entamoeba-ELISA (sensitivity= 54%). Five of the 115 samples without cysts of E. histolytica or E. dispar were Entamoeba ELISA positive (specificity= 96%). All samples were negative to the E. histolytica-specific ELISA. The correlation between the microscopical identification and Entamoeba-ELISA was not good, it may be due to an overvaluation of the of the morphological diagnosis. (authors)

  8. Fluorescence polarization assay, competitive enzyme-linked immunosorbent assay (ELISA-C and indirect ELISA for the diagnosis of brucellosis in buffaloes (Bubalus bubalis Teste de polarização fluorescente, teste imunoenzimático competitivo (ELISA-C e ELISA indireto para o sorodiagnóstico da brucelose em búfalos (Bubalus bubalis

    Directory of Open Access Journals (Sweden)

    Lília Márcia Silva Paulin

    2012-09-01

    Full Text Available The objective of the present study was to compare the performance of three serological tests for diagnosis of Brucella abortus infections in buffaloes (Bubalus bubalis. Serum samples collected from 696 adult females were submitted to the competitive enzyme-linked immunosorbent assay (ELISA-C, (I-ELISA, fluorescence polarization test (FPA, 2-mercaptoethanol test (2-ME and complement fixation test (CFT. The gold standard was the combination of CFT and 2-ME, considering as positive the reactors in both CFT and 2-ME, and as negative those non-reactors. ROC analyses were done for C-ELISA, I-ELISA and FPA and the Kappa agreement index were also calculated. The best combinations of relative sensitivity (SEr and relative specificity (SPr and Kappa were given by C-ELISA (96.9%, 99.1%, and 0.932, respectively and FPA (92.2%, 97.6 and 0.836, respectively. The C-ELISA and FPA were the most promising confirmatory tests for the serological diagnosis of brucellosis in buffaloes, and for these tests, cut-off values for buffaloes may be the same as those used for bovines.O presente estudo objetivou comparar o desempenho de três testes para o sorodiagnóstico da Brucella abortus em búfalos (Bubalus bubalis. Soros de 696 fêmeas bubalinas adultas foram submetidos aos testes: teste imunoenzimático indireto (ELISA-I, teste imunoenzimático competitivo (ELISA-C, teste de polarização fluorescente (TPF, 2-mercaptoetanol (2-ME e teste de fixação do complemento (FC. Foi empregada, como gold standard, a combinação de dois testes, FC e 2-ME. A curva ROC foi construída para os três testes: ELISA-I, ELISA-C e PF e, com base nos resultados desta análise, foi calculado o índice de concordância Kappa para cada teste. As melhores combinações de sensibilidade (Sr e especificidade (Er e os melhores resultados de Kappa foram alcançados pelo ELISA-C (96,9%, 99,1% e 0,932, respectivamente, seguido pelo PF (92,2%, 97,6%, e 0,836, respectivamente. Concluiu-se que os

  9. RT-PCR-ELISA as a tool for diagnosis of low-pathogenicity avian influenza

    DEFF Research Database (Denmark)

    Dybkær, Karen; Munch, Mette; Handberg, Kurt; Jørgensen, Poul Henrik

    A one-tube reverse transcriptase/polymerase chain reaction coupled with an enzyme-linked immunosorbent assay (RT-PCR-ELISA) was developed for the rapid detection of avian influenza virus (AIV) in clinical specimens. A total of 419 swab pools were analyzed from chickens experimentally infected with...... low-pathogenicity AIV, from wild aquatic birds, and from domestic ducks. The AIV was detected in 32 swab pools by RT-PCR-ELISA compared to 23 by virus isolation (VI) in embryonated specific pathogen free (SPF) chicken eggs. Thus, 39% more specimens were positive by RT-PCR-ELISA than by VI. Two of the...

  10. Performance indexes of a dot-enzime-linked immunosorbent assay (dot-ELISA and an ezyme-linked immunosorbent assay (IgG-ELISA for field surveys of new world leishmaniasis Índices de desempenho do dot-ELISA e do IgG-ELISA, em inquéritos soroepidemiológicos da leishmaniose do Novo Mundo

    Directory of Open Access Journals (Sweden)

    M. Carolina S. Guimarães

    1991-10-01

    Full Text Available Diagnostic performance indexes of sensitivity, specificity, positive predictive value and efficiency were determined for dot-ELISA and IgG-ELISA tests in 340 leishmaniasis sera. Sensitivity of the dot-ELISA was significantly lower than IgG-ELISA's; the two tests had indexes of specificity and positive predictive value of the same magnitude. Seventy-eight sera gave a negative dot-ELISA test result and a positive IgG-ELISA test result. When sera were classified according to different criteria as how to interpret this diversity, the kappa statistic did not corroborate the classification indicating that the two tests display a substantial strength of agreement. The results presented indicate that performance indexes accrued in a survey where variables arc well known may be extrapolated to other population studies if the disease presents itself as highly prevalent (due to a selection bias or not and may be expected to discriminate a disease status among test positives.Índices de desempenho diagnóstico de sensibilidade, especificidade, valor preditivo positivo e eficiência foram determinados nas reações de dot-ELISA e IgG-ELISA em 340 soros de leishmaniose mucocutânea. A sensibilidade do dot-ELISA foi significativamente mais baixa que a da IgG-ELISA e os dois testes tiveram índices de especificidade e de valor de predição positivo da mesma magnitude. Setenta e oito soros tinham resultado negativo à reação de dot-ELISA e resultado positivo ao IgG-ELISA. Quando os soros foram examinados de acordo com diferentes critérios de análise, quanto a esta discrepância a estatística kappa não corroborou esta hipótese indicando que os dois testes concordavam substancialmente. Os resultados apresentados indicam que os índices de desempenho diagnóstico obtidos em trabalhos de campo onde haja boa caracterização das variáveis podem ser extrapolados a outros estudos populacionais desde que a doença se mostre de alta prevalência (devido a um vi

  11. Optimización de la cadena de suministro: Implantación de un sistema SMI( Supplier Managed Inventory) en una empresa de fabricación de bebidas alcohólicas

    OpenAIRE

    Montero Ortiz, Roberto

    2015-01-01

    La elevada exigencia competitiva en el mundo empresarial es el principal motor del cambio. Para tener éxito en un entorno tan agresivo, es necesario llevar a la empresa al siguiente nivel. No es suficiente con integrar todos los actores internos dentro de una cadena eficiente; la clave está en los externos. Una vez hemos optimizado nuestros procesos internos, debemos poner el foco en todos aquellos agentes externos que escapan a nuestro control directo. Es necesario cambiar de ...

  12. Estructura y función de la cadena productiva de carne de bovino en la ganadería ejidal de Tecpatán, Chiapas, México

    Directory of Open Access Journals (Sweden)

    J. Calderón

    2012-01-01

    Full Text Available El objetivo de la presente investigación fue analizar la estructura y el funcionamiento de la cadena productiva de carne bovina en el municipio de Tecpatán, Chiapas, México. Se aporta información cualitativa y cuantitativa orientada a comprender y consolidar las alianzas entre actores de la cadena productiva. Los resultados muestran la existencia de los eslabones de producción de bovinos en pie, comercialización, transformación y venta de carne. Los servicios de apoyo están dados por tres asociaciones ganaderas (ag, dos rastros municipales y un rastro tipo inspección federal. Existen cuatro circuitos de comercialización bien diferenciados en cuanto a número y tipo de actores que participan en la relación de producción- compra-venta, producto comercializado y mercado final de destino. El circuito que involucra mayor cantidad de animales en pie es el que comercializa becerros destetados para engorda. En todos los circuitos, el mayor margen de comercialización lo obtienen los intermedia-rios que participan en el eslabón de comercialización. Los beneficiarios son principalmente acopiadores mayoristas regionales y nacionales del circuito más corto. En el aspecto organizacional, los productores cuentan con AG locales y regionales, mientras que los agentes de la comercialización no cuentan con ningún tipo de organización. Se requiere una política de apoyo permanente para consolidar alianzas entre los actores, así como una estrategia de competitividad orientada a diferenciar los productos. De esta forma, la cadena productiva actual podría transitar poco a poco hacia una cadena de valor.

  13. Validación de un ELISA para la cuantificación de antitoxina tetánica en suero humano

    Directory of Open Access Journals (Sweden)

    Rolando Ochoa

    2000-12-01

    Full Text Available Se desarrolló un ensayo inmunoenzimático en fase sólida (ELISA de tipo indirecto para la cuantificación de antitoxina tetánica en suero humano, con el empleo de un estándar previamente calibrado. Las imprecisiones intra e interensayo fueron de alrededor del 10%. Este ensayo mostró una excelente exactitud, con valores de recuperación entre 90% y 110%. Las desviaciones del paralelismo estuvieron por debajo del 10%; éstas se evaluaron mediante diluciones que involucraban el rango de trabajo de la curva estándar. El límite de detección fue 0,0016 Unidades Internacionales por mL (UI/mL. El ensayo mostró una buena correlación con la prueba de neutralización (R2=0,9862. La ecuación de ajuste lineal fue: ELISA = 0,993 ensayo de neutralización – 0,2143. Se cuantificó la antitoxina tetánica en muestras de suero de 408 niños entre 1 a 5 años de edad,aleatoriamente seleccionados en Ciudad de La Habana y cada edad se analizó por separado. La mayor respuesta se alcanzó a los 2 años de edad, al completarse el esquema básico de inmunización. Todos los niños presentaron valores superiores al nivel mínimo protector (0,01 UI/mL.

  14. The time course of the specific antibody response by various ELISAs in pigs experimentally infected with Toxoplasma gondii

    DEFF Research Database (Denmark)

    Lind, Peter; Haugegaard, J.; Wingstrand, Anne;

    1997-01-01

    With the aim of developing routine serological tests for monitoring the Toxoplasma infection status of Danish swine herds, four ELISAs based on tachyzoite antigen were set up: (1) an indirect ELISA for IgG-antibody; (2) a blocking ELISA for antibody to the membrane antigen, P-30; (3) an indirect ...

  15. Evaluation of a blocking ELISA for screening of antibodies against porcine reproductive and respiratory syndrome (PRRS) virus

    DEFF Research Database (Denmark)

    Sørensen, K.J.; Bøtner, Anette; Madsen, E.S.; Strandbygaard, Bertel; Nielsen, Jens

    A blocking Elisa was developed for the detection of antibodies against PRRS virus with a view to satisfying the need for examination of blood samples on a large scale. The test was evaluated in comparison with an indirect Elisa and the immunoperoxidase monolayer assay. The blocking Elisa was...

  16. Las cadenas léxicas como exponentes de coherencia textual en la pragmática escrita de la interlingua

    Directory of Open Access Journals (Sweden)

    Francisca Suau Jiménez

    2011-04-01

    Full Text Available Normal 0 21 false false false ES X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} La pragmática de la interlingua, aunque de reciente aparición dentro de la teoría que explica la adquisición de una L2, ha demostrado que permite analizar el comportamiento pragmalingüítico de los hablantes no-nativos de una lengua de modo más preciso que otras aproximaciones sociopragmáticas. A través de los resultados de una investigación empírica pretendemos contribuir a esta línea de trabajo aportando las conclusiones de un estudio realizado con hablantes nativos (HN y no-nativos (HNN. A partir del estudio comparativo de las cadenas de cohesión léxica que los dos grupos de sujetos utilizan en la construcción de la coherencia textual pretendemos averiguar si la competencia retórica de los HNN seria paralela a su competencia gramatical y, por lo tanto, inferior a la de los HN, o si, por el contrario, no es así, debido a la posible transferencia de patrones retóricos de la LI en los HNN. Para ello hemos aplicado el modelo de redes de cadenas léxicas de Hoey (1991. Los resultados del trabajo sugieren que arribas competencias, la gramatical y la retórica no contemplan paralelismo alguno en el uso que hacen ambos

  17. Prospects of eLISA for detecting Galactic binary black holes similar to GW150914

    Science.gov (United States)

    Seto, Naoki

    2016-07-01

    We discuss the prospects of eLISA for detecting gravitational waves (GWs) from Galactic binary black holes (BBHs) similar to GW150914. For a comoving merger rate that is consistent with current observation, eLISA is likely to identify at least one BBH with a sufficient signal-to-noise ratio. In addition, eLISA has a potential to measure the eccentricity of the BBH as small as e ˜ 0.02, corresponding to the residual value e ˜ 10-6 at 10 Hz. Therefore, eLISA could provide us with a crucial information to understand the formation processes of relatively massive BBHs like GW150914. We also derive a simple scaling relation for the expected number of detectable Galactic BBHs.

  18. A liquid phase blocking ELISA for the detection of antibodies against infectious bronchitis virus

    Directory of Open Access Journals (Sweden)

    Cardoso T.C.

    1999-01-01

    Full Text Available A liquid phase blocking ELISA (LPB-ELISA was developed for the detection and measurement of antibodies against infectious bronchitis virus (IBV. The purified and nonpurified virus used as antigen, the capture and detector antibodies, and the chicken hyperimmune sera were prepared and standardized for this purpose. A total of 156 sera from vaccinated and 100 from specific pathogen-free chickens with no recorded contact with the virus were tested. The respective serum titers obtained in the serum neutralization test (SNT were compared with those obtained in the LPB-ELISA. There was a high correlation (r2 = 0.8926 between the two tests. The LPB-ELISA represents a single test suitable for the rapid detection of antibodies against bronchitis virus in chicken sera, with good sensitivity (88%, specificity (100% and agreement (95.31%.

  19. Ag-ELISA for the detection of trypanosome infections in cattle and camels

    International Nuclear Information System (INIS)

    This document describes very briefly the two elisa kits used for the detection of trypanosome infections in cattle and camels. Results on the specificity and sensitivity of assays performed with the kits are summarized. 9 refs

  20. The use of the antigen ELISA for monitoring tsetse and trypanosomosis control programmes in Zimbabwe

    International Nuclear Information System (INIS)

    Blood and serum samples from cattle originating from tsetse free and tsetse infested areas were analyzed using the Buffy Coat technique and an ELISA to detect trypanosomes and trypanosomal antigens, respectively. The results of the two tests were compared and apparent sensitivity and trypanosomal prevalence were calculated. The BCT seemed to be the most suitable test to detect acute infections, while the antigen capture ELISA (Ag-ELISA) was able to detect more chronic infections. The specificity of the Ag-ELISA was found to be very good, but the sensitivity of the test should be improved. One way to detect more T. congolense and T. vivax infections was to lower the cut-off point of percent positivity from 10 to 5%. (author). 1 ref., 1 fig., 7 tabs

  1. Prospects of eLISA for Detecting Galactic Binary Black Holes Similar to GW150914

    CERN Document Server

    Seto, Naoki

    2016-01-01

    We discuss the prospects of eLISA for detecting gravitational waves (GWs) from Galactic binary black holes (BBHs) similar to GW150914. For a comoving merger rate that is consistent with current observation, eLISA is likely to identify at least one BBH with a sufficient signal-to-noise ratio. In addition, eLISA has a potential to measure the eccentricity of the BBH as small as $e\\sim 0.05$, corresponding to the residual value $e\\sim 10^{-6}$ at 10Hz. Therefore, eLISA could provide us with a crucial information to understand the formation processes of relatively massive BBHs like GW150914. We also derive a simple scaling relation for the expected number of detectable Galactic BBHs.

  2. Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA

    Directory of Open Access Journals (Sweden)

    Alexandra H. Heussner

    2010-06-01

    Full Text Available A monoclonal antibody specific to ochratoxin B (OTB was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD for OTB of 3 µg/L (8 nM, a limit of quantification (LOQ of 3.7 µg/L (10 nM, and a 50% inhibitory concentration (IC50 of 150 nM. Due to very low cross-reactivity to OTA (2.7% and structurally related molecules (0%, this OTB-ELISA was found to be suitable to detect OTB with excellent precision in different matrices, i.e., beer, coffee and wine. Therefore, this OTB-ELISA will allow screening of OTB in food and feed products.

  3. Indirect ELISA with Recombinant GP5 for Detecting Antibodies to Porcine Reproductive and Respiratory Syndrome Virus

    Institute of Scientific and Technical Information of China (English)

    Yan Chen; Hong Tian; Jian-Hui He; Jin-Yin Wu; You-jun Shang; Xiang-tao Liu

    2011-01-01

    Porcine reproductive and respiratory syndrome is caused by the PRRS virus(PRRSV), which has six structural proteins(GP2, GP3, GP4, GP5, M and N). GP5 and N protein are important targets for serological detection by enzyme-linked immunosorbent assay(ELISA)and other methods. Toward this goal, we developed an indirect ELISA with recombinant GP5 antigens and this method was validated by comparison to the LSI PRRSV-Ab ELISA kit. The results indicated that the optimal concentration of coated recombinant antigen was 0.2 μg/well for a serum dilution of 1:40. The rate of agreement with the LSI PRRSV-Ab kit was 88.7%(266/300). These results support the potential use of recombinant GP5 as an antigen for indirect ELISA to detect PRRSV antibodies in pigs.

  4. Evaluation of CSFV Antibody ELISAs for the differentiation of infected from vacci-nated animals

    DEFF Research Database (Denmark)

    Schroeder, Sabine; Blome, Sandra; Koenen, Frank;

    countries and out-breaks occurred recently e.g. in Germany, France, Hungary, Romania, Bulgaria, and the Slovak Republic. Preventive vaccination is prohibited within the EU, but emergency vaccination can be part of the strategy in case of a contingency. Using conventional vaccines, differentiation of...... their diagnostic sensitivity as well as for their potential in differentiating between infected and marker vaccinated animals. Two newly available ELISAs were included into the tests, the Priocheck® CSFV Erns ELISA, a special DIVA test, and the LDL Pigtype® CSFV Antibody ELISA. An inter......, specificity, repeatability, and reproducibility were calculated and conclusions were drawn on the feasibility of using existing Ab-ELISAs for DIVA testing. The results will be presented at the meeting. Acknowledgement: This work was supported by the EU Network of Excellence, EPIZONE (Contract No FOOD-CT-2006-016236)....

  5. Parts Per Trillion Detection of 7-Aminonitrazepam by Nano-Enhanced ELISA

    Directory of Open Access Journals (Sweden)

    Feng Xue

    2013-09-01

    Full Text Available It is challenging to detect 7-aminonitrazepam (7-ANZP residue in animal tissues simply and sensitively by the enzyme-linked sorbent immunoassay (ELISA method. This paper demonstrates that utilizing a bioconjugate of gold nanoparticles and enzyme-labeled antibody as a signal probe increases the sensitivity of a traditional ELISA for 7-ANZP by nearly 20 times. The sensitivity of this ELISA for 7-ANZP was 5.6 pg/mL in buffer, and the limit of detection (LOD of 0.18 µg/kg for 7-ANZP in urine could be achieved after the urine samples were simply hydrolyzed and diluted by buffer. This simple and sensitive method has potential application for improving the sensitivity of ELISA methods against various small molecules.

  6. Inkassofirma ähvardab tuhandeid Elisa kliente aegunud nõuetega / Lauri Linnamäe

    Index Scriptorium Estoniae

    Linnamäe, Lauri

    2008-01-01

    Julianus Inkasso nõuab Elisa praegustelt ja kunagistelt klientidelt aegunud võlgade tasumist koos intressidega. Kommenteerib Urmas Volens. Vt. samas: Julianus Inkasso: aegumist saab tõlgendada mitmeti

  7. ELISA with double antigen sandwich for screening specific serum anti-TP antibody in blood donors

    International Nuclear Information System (INIS)

    Objective: To select a sensitive and specific laboratory examination suitable for screening serum anti-TP antibody in blood donors. Methods: The serum anti-TP antibody in 11271 blood donors were detected using ELISA with double antigen sandwich and the outcomes were compared with those using RPR assay. The conflicting specimen were confirmed by repeating the test with TPHA assay. Results: The positive rates of serum anti-TP antibody by ELISA with double antigen sandwich and RPR was 0.36% (41/11271) and 0.26% (29/11271), respectively. The coincidence of the detecting outcomes by ELISA with double antigen sandwich and RPR with TPHA was 97.5% (40/41) and 63.41%(26/41) respectively. Conclusion: Compared with RPR assay, ELISA with double antigen sandwich has higher sensibility and specificity for screening serum anti-TP antibody in blood donors

  8. Monoclonal antibodies against proteins of the IBR virus nucleocapside and their assessing by ELISA

    Directory of Open Access Journals (Sweden)

    Jeannette Navarrete 0.

    2011-12-01

    Full Text Available Monoclonal antibodies were produced against the nucleocapside of a field IBR virus strain. The virus was growth in MOBK cell line. Viral components were purified and concentrated in a continuous 30% sacarose gradient followed by chemical precipitation. Nucleocapsides were run in a 10% SDS-PAGE gel. Positive hybridomes were tested using a direct ELISA developed in our laboratory. As a result eigth ELISA positive clones were obtained, from these five were also positive to seronetralization and immunodot. The clones recognize a 39.8Kda protein. Aditionally, a capture-ELISA was developed using the monoclonal antobodies from this research. This ELISA is useful to detect a reference as a field nm virus strain.

  9. ELISA and the Joint FAO/IAEA Division: Accomplishments and opportunities

    International Nuclear Information System (INIS)

    The enzyme linked immunosorbent assay (ELISA) is an inexpensive yet sensitive and specific tool in the diagnosis of livestock diseases. The use of the ELISA can be associated with many efforts at reducing the enormous wastage of animals caused by infectious diseases. The use of this technology in the developing countries was recommended in 1983 by a consultants meeting called by the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna. Since then, enormous resources have been committed towards the development and standardization of ELISA kits and their application, mostly in Africa, Asia and Latin America. The IAEA introduced training programmes, initiated regional research efforts, sponsored technical co-operation projects, provided ELISA readers and other equipment and dispatched experts/trainers to assist laboratories in developing countries. International standards for ELISA data expression and evaluation, reagent reference standards and an external quality assurance programme have been developed with assistance from consultants so that laboratories can meet minimal ELISA performance standards. Despite these efforts, the proper validation of ELISAs for application in some geographical areas is still difficult. Unique specificity problems occur because of unknown substances in sera of test animals. The application of ELISA in the field is difficult, and usually laboratories are required for the analysis of samples. Even in the laboratory the technique is easily 'abused', resulting in invalid data. Therefore, the ELISAs of the future that will be most effective in control and eradication of livestock diseases in developing countries should be simpler, more specific, less prone to error and portable for use in remote areas. They will probably consist of membrane based ELISA technologies accompanied by handheld portable touch screen computers for data management. Inexpensive means of identification of tested animals are becoming available

  10. Comparison of Wright Agglutination Test and ELISA in Diagnosis of Brucellosis

    Directory of Open Access Journals (Sweden)

    Ansarinia, H.

    2014-06-01

    Full Text Available Background and Objective: In our country, the Wright test routinely is used for diagnosing brucellosis. Because of its low sensitivity, the range of false-negative results is high. Therefore, we aimed at comparing Wright and ELISA in the people suspected brucellosis. Material and Methods: The results of Wright, 2ME, Coombs Wright tests were compared with Anti-Brucella IgG, Anti-Brucella IgM. Of 1183 subjects referred for Wright test, 148 of them were investigated for Coombs Wright and 228 for 2ME Wright. In addition to Wright test for 32 cases, Brucella IgG and IgM classes were also experimented. Results: Wright test was negative in 95.4% of cases. Of these negative results, 2.3% were positive for Coombs Wright. Eight-point-five percent of the cases were positive for Coombs Wright test and 4.7% for 2ME Wright test. Sixteen cases were negative for both Wright and ELISA. In 8 cases of Wright-negative, ELISA IgM class was positive and IgG class was negative, and in 4 cases of Wright-negative, ELISA IgM was negative and IgG was positive. About 4 cases of Wright-positive, IgM and IgG antibody classes were positive. Conclusion: Due to the mismatch between the results of Wright agglutination test and ELISA method and with regard to availability, high sensitivity and determining the type of antibody classes in ELISA, it is focused on ELISA method for brucellosis diagnosis. Keywords: Brucellosis; Wright; ELISA

  11. Application of ELISA for diagnosing and investigating the epidemiology of trypanosomiasis in buffaloes in north Vietnam

    International Nuclear Information System (INIS)

    An antigen-detection enzyme immunoassay (Ag-ELISA) based on a Trypanosoma evansi specific monoclonal antibody was used for the detection of circulating antigens in experimentally infected buffaloes and in buffaloes kept in different geographical regions of Vietnam. In experimental infections, circulating antigens were detected around 7 to 14 days after infection but the levels detected were considerably lower than those in sera collected from the field, suggesting that the parasite isolate used became highly adapted to mice thorough passage. In a study of field sera the agreement between the Ag-ELISA and parasitological methods for detecting infected cases was 80% and the apparent specificity of the test was 90%. In these and other studies the Ag-ELISA consistently detected many more infected cases than did the conventional parasitological methods. In most cases animals whose sera reacted in the Ag-ELISA also had trypanosome specific antibodies in their circulation. Treatment of infected animals with a trypanocidal drug in most cases resulted in a significant drop in trypanosome antigen levels but their status with respect to trypanosomal antibodies remained unchanged. Seroepidemiological surveys using Ag-ELISA and Ab-ELISA and base on an analysis of around 1000 sera collected from different regions of Vietnam demonstrated prevalence ranging between 17% and 50% with no apparent relationship to geographical region. However, prevalence of infection was generally higher in summer (rainy season), when fly activity is highest. It is concluded that serological methods based on Ag-ELISA and Ab-ELISA are valuable adjuncts to clinical and parasitological methods for diagnosing trypanosomiasis, studying the epidemiology of the disease and monitoring the effectiveness of control programmes. (author). 12 refs, 2 figs, 3 tabs

  12. Antigenisitas, Sensitivitas, dan Spesifisitas Protein Toxocara canis pada Pemeriksaan Antibodi Serum Mencit dengan Indirect-ELISA

    OpenAIRE

    Sri Subekti Bendryman; Kusnoto .; Tutik Juniastuti

    2015-01-01

    The aim of this research were to determine antigenicity, sensitivity, and specificity of Toxocara canisprotein used as antigen in indirect-ELISA for the detection antibody against the worm in the infected hostin order to proper diagnose kit. The design used was true experimental, with Post-test Only ControlGroups Design. Mouse was immunized with various worm homogenates used to antigenicity, sensitivityand specificity tests of T. canis protein with indirect-ELISA technique. The independence v...

  13. Comparison of ELISA, SPACE, and electron microscopy for the routine diagnosis of rotavirus infection.

    OpenAIRE

    Wall, R A; Mellars, B J; Luton, P.; Boulding, S

    1982-01-01

    Previous studies on the serological diagnosis of rotavirus infection have utilised locally produced antibodies. In this study we have compared two commercially produced assays, an ELISA (Rotazyme, Abbott) and a newly developed assay--solid phase aggregation of coupled erythrocytes (SPACE) (Wellcome Research Laboratories), with electron microscopy (EM). The SPACE test appeared less sensitive than EM. The ELISA was shown to be as sensitive as EM but more versatile. Our experience suggests that ...

  14. Methodological aspects of ELISA analysis of thioredoxin 1 in human plasma and cerebrospinal fluid.

    Science.gov (United States)

    Lundberg, Mathias; Curbo, Sophie; Reiser, Kathrin; Masterman, Thomas; Braesch-Andersen, Sten; Areström, Irene; Ahlborg, Niklas

    2014-01-01

    Thioredoxin-1 (Trx1) is a protein antioxidant involved in major cellular processes. Increased plasma levels of Trx1 have been associated with human diseases suggesting that Trx1 is a marker for oxidative stress with putative clinical use. However, the reported mean levels of Trx1 in the control cohorts vary a hundred-fold between studies (0.8-87 ng/ml), possibly due to methodological differences between the capture ELISA used in the different studies. The aim of this study was to investigate methodological aspects related to the ELISA measurement of Trx1. ELISAs utilizing different capture and detection combinations of antibodies to Trx1 and as well as recombinant human (rh) Trx1 standards from two sources were characterized. The different ELISAs were subsequently used to measure Trx1 in human plasma and cerebrospinal fluid samples (CSF) from healthy donors and from patients with various neurological diagnoses. The Trx1 standards differed in their content of monomeric and oligomeric Trx1, which affected the ELISAs composed of different antibody combinations. Thus, the levels of Trx1 determined in human plasma and CSF samples varied depending on the antibody used in the ELISAs and on the rhTrx1 standard. Furthermore, the relevance of preventing interference by heterophilic antibodies (HA) in human plasma and CSF was investigated. The addition of a HA blocking buffer to human samples drastically reduced the ELISA signals in many samples showing that HA are likely to cause false positive results unless they are blocked. In conclusion, the study shows that the design of a Trx1 ELISA in regards to antibodies and standards used has an impact on the measured Trx1 levels. Importantly, analyses of human plasma and CSF without preventing HA interference may obscure the obtained data. Overall, the results of this study are crucial for the improvement of future studies on the association of Trx1 levels with various diseases. PMID:25075746

  15. Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever

    OpenAIRE

    Paredes Julio Cesar Muñoz; Oliveira Liliane Guimarães; Braga Alexandre de Carvalho; Trevisol Iara Maria; Roehe Paulo Michel

    1999-01-01

    An indirect enzyme linked immunoassay (ELISA-I) was developed and standardized for the serological diagnosis of classical swine fever (CSF). For the comparison, nine hundred and thirty-seven swine serum samples were tested by serum neutralization followed by immunoperoxidase staining (NPLA), considered as the standard. Of these, 223 were positive and 714 negative for neutralizing antibodies to classical swine fever virus (CSFV). In relation to the NPLA, the ELISA-I presented a 98.2% sensitivi...

  16. ELISA assays for the detection of Bothrops lanceolatus venom in envenomed patient plasmas.

    Science.gov (United States)

    Rodriguez-Acosta, A; Uzcategui, W; Azuaje, R; Giron, M E; Aguilar, I

    1998-01-01

    A double antibody sandwich enzyme linked immunosorbant assay (ELISA) was carried out to detect Bothrops Ianceolatus venom in plasma from envenomed patients at various time intervals (0, 6, 12, 18 and 24 hrs). The test could detect Bothrops lanceolatus levels up to 12 ng/mL of envenomed patient plasmas. Elaboration of an easy, fast and species-diagnostic based on this ELISA technique useful to physicians is discussed. PMID:11845439

  17. Evaluation of two indigenous rapid and two ELISA assays for the diagnosis of HIV infection India

    OpenAIRE

    H S Iqbal; Solomon, S; K G Murugavel; S S Solomon; P. Balakrishnan

    2012-01-01

    Purpose: Human immunodeficiency virus (HIV) diagnostic tests are being used extensively in India. However, the evaluation data on these assays are very limited. The present study evaluates indigenous HIV test kits manufactured in India. Materials and Methods: A total of 200 characterised specimens were assayed with Comb AIDS - RS Advantage HIV 1+2 Immunodot Test, Enzaids HIV 1+2 ELISA test, Enzaids Duet HIV Antigen+antibody ELISA test and Signal HIV Flow Through HIV 1+2 test kits. Performance...

  18. Relation between Ascites Syndrome Incidence and Infectious Bronchitis in Broiler Chickens by ELISA Method

    OpenAIRE

    Adel Feizi; Mehrdad Nazeri

    2011-01-01

    Infectious bronchitis is an acute viral disease with high contagious and mortality among chicks. The aim of this study was to survey of relation between ascites syndrome incidence and infectious bronchitis in broiler chickens by ELISA method in Iran. Eight Ross strain broiler farm affected by infectious bronchitis were selected in this study. Blood samples were gathered early stages of disease and blood sampling was repeated two times with seven days interval. ELISA serologic test was used fo...

  19. Constraining stellar binary black hole formation scenarios with eLISA eccentricity measurements

    CERN Document Server

    Nishizawa, Atsushi; Berti, Emanuele; Klein, Antoine

    2016-01-01

    A space-based interferometer such as eLISA could observe few to few thousands progenitors of black hole binaries (BHBs) similar to those recently detected by Advanced LIGO. Gravitational radiation circularizes the orbit during inspiral, but some BHBs retain a measurable eccentricity at the low frequencies where eLISA is most sensitive. The eccentricity of a BHB carries precious information about its formation channel: BHBs formed in the field, in globular clusters, or close to a massive black hole (MBH) have distinct eccentricity distributions in the eLISA band. We generate mock eLISA observations, folding in measurement errors, and using Bayesian model selection we study whether eLISA measurements can identify the BHB formation channel. We find that a handful of observations would suffice to tell whether BHBs were formed in the gravitational field of a MBH. Conversely, several tens of observations are needed to tell apart field formation from globular cluster formation. A five-year eLISA mission with the lon...

  20. Using of competitive ELISA in detection of brucella antibodies in cattle sera

    Directory of Open Access Journals (Sweden)

    M. I. Al-Farwachi

    2009-01-01

    Full Text Available Competitive ELISA test was used in this study to identify brucella antibodies in cattle sera in Mosul, and assessing the results that are given by Rose-Bengal test. Results of testing 126 cattle sera revealed that the seroprevalence was 16.7% using C-ELISA and 18.3% using RBT. There were differences in seroprevalence according to sex of animals, females showed higher seroprevalence than males, it was 19.1% and 20.2% in females and 9.4% and 12.5% in males by C-ELISA and RBT respectively. Also there were differences in seroprevalence according to age of animals and pregnancy status of females. Kappa value revealed that there was little agreement between C-ELISA and RBT, (Kappa value was 0.229. RBT was not accurate in giving real seroprevalence, since there were 13 false-negative samples from 21 samples positive with C-ELISA, and there were 15 false-positive samples from 105 samples negative with C-ELISA. Results of tube agglutination test and 2-Mercaptoethanol test indicated a decline of titers from those of previous studies, lower titer of TAT was 1/10 (19.05% and most of titers were lower than 1/40 (76.2% in 2-Me test. This study concluded that the brucella antibodies in cattle sera in Mosul was increased from previous years and most of cases were acute.

  1. Development of an Enzyme-linked Immuno-Sorbent Assay (ELISA Method for Carbofuran Residues

    Directory of Open Access Journals (Sweden)

    Zhenlin Xu

    2008-04-01

    Full Text Available The haptens 4-[[(2,3-dihydro-2,2-dimethyl-7-benzofuranyloxycarbonyl]-amino]butanoic acid (BFNB and 6-[((2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy-carbonylamino]hexanoic acid (BFNH were synthesized and then used to develop a rapid,specific and sensitive ELISA method to determine residues of the pesticide carbofuran in avariety of matrices. A hybridoma cell line (5D3 producing anti-carbofuran monoclonalantibodies (MAbs was also established. Based on the MAbs in combination with theheterologous hapten BFNH coupled to either horseradish peroxidase (HRP or ovalbumin(OVA, four ELISAs (formats I-IV for the quantification of carbofuran were developedand compared. Among them, the optimized format II (the conjugate-coated directcompetitive ELISA showed the best characteristics, with an IC50 value of 18.49 ng/mL, alimit of detection of 0.11 ng/mL and the shortest assay time (1 h. This ELISA method wasthen applied to the determinations of carbofuran in environmental water, soil and foodsamples. The relative standard deviations (R.S.D.s ranged from 1.8% to 21.3% and themean recoveries were 104.6%, 108.3%, 106.3% and 100.1% for water, soil, lettuce andcabbage, respectively. Thus, the ELISA method of format II exhibited the potential todevelop commercial ELISA kits for a rapid detection of carbofuran for human health andenvironmental safety.

  2. Validation of an ELISA for the quantitation of lanoteplase, a novel plasminogen activator.

    Science.gov (United States)

    Stouffer, B; Habte, S; Vachharajani, N; Tay, L

    1999-11-01

    An ELISA was developed and validated for the quantitation of lanoteplase in human citrated plasma. The ELISA employed a monoclonal anti-lanoteplase antibody absorbed onto 96-well microtiter plates to capture lanoteplase in citrated human plasma samples containing PPACK, a protease inhibitor. The captured lanoteplase was detected using a biotinylated rabbit anti-lanoteplase polyclonal antibody. The standard curve range in human plasma for the ELISA was 7-100 ng/ml. Assessment of individual standard curve variability indicated reproducible responses with r2 values of > or = 0.985. The accuracy (% DEV) and precision (%RSD) estimates for the ELISA based on the predicted values from quality control (QC) samples were within 7.3% and 11%, respectively. Cross-reactivity with t-PA was determined to be less than 11% by ELISA. The stability of lanoteplase was established in human citrated PPACK plasma for 24 hours at 4 degrees C, for 2 months at -20 degrees C, for 22 months at -70 degrees C, three weeks at room temperature, and through four freeze/thaw cycles. To quantify lanoteplase plasminogen activator (PA) activity, a commercially available chromogenic activity assay was also validated. This method and its application is described briefly here. The lanoteplase ELISA as well as the commercial activity method were successfully employed to evaluate the pharmacokinetic parameters of lanoteplase in support of clinical Phase II/III studies. PMID:10595857

  3. Evaluation of serum and milk ELISAs for paratuberculosis in Danish dairy cattle

    DEFF Research Database (Denmark)

    Klausen, Joan; Huda, A.; Ekeroth, Lars; Ahrens, Peter

    concurrently from six dairy herds infected with MAP and from two dairy herds without history of infection with MAP. A cut-off value of 7 OD% was used in the ELISAs. At this cut-off value, all six culture-positive herds were positive in the serum ELISA but one was negative in the milk ELISA. All six culture......A milk and a serum ELISA for detection of antibodies against Mycobacterium avium ssp. paratuberculosis (MAP) were evaluated against the complement-fixation test (CFT) and culture of faecal samples from 580 cows collected between August 1996 and December 1996. Milk and serum were obtained......-positive herds were positive in the CFT. In the two culture-negative herds, the serum and the milk ELISA deemed all serum samples negative at this cut-off value, whereas four serum samples from one of these herds were positive in the CFT. The highest cut-off value enabling the milk ELISA to record all six...

  4. Development of an ELISA Method Detecting Strobilurin Fungicide through the Immune Magnetic Beads Collecting Samples

    Directory of Open Access Journals (Sweden)

    Xun C. Huang

    2015-02-01

    Full Text Available Trifloxystrobin, one of the strobilurin fungicides, is active as inhibitor of low toxicity and easy degradation against a wide range of fungal plant pathogens. In the experiment, Hydroxy from magnetic microsphere and amino of anti-Trifloxystrobin antibody could condensate to form Immune Magnetic Beads (IMB, which could specially capture trifloxystrobin in the food matrix and rapidly separated it through the magnetic field. An ELISA method (IMB-ELISA was developed to detect the captured fungicide through examining the best reactive concentration, sensitivity, specificity, matrix effect and recovery rate. The half maximal inhibitory concentration (IC50 value obtained by the IMB-ELISA method was 2.28 &mug/mL (R2 = 0.9837 against 12.57 &mug/mL of conventional ELISA method (R2 = 0.9747. The recovery rates were 90.12-103.65% and the Coefficients of Variation (CVs were in the range of 4.5-11.2%. In three kind of citrus samples, there was only low cross-reactivity in IMB-ELISA method by detecting matrix effects. Based on above experiment, the IMB-ELISA method was an efficient and sensitive method for Trifloxystrobin detection.

  5. Use of ELISA to monitor bacterial kidney disease in naturally spawning chinook salmon.

    Science.gov (United States)

    O'Connor, Glenda; Hoffnagle, Timothy L

    2007-09-14

    Bacterial kidney disease (BKD) caused by Renibacterium salmoninarum (Rs) is a serious problem among Pacific Northwest salmon hatcheries and has raised concerns that salmon reared in hatcheries may spread BKD to natural populations. In order to monitor the potential spread of this disease to salmon spawning in nature, a method must be available to collect and analyze tissues from naturally spawning salmon. Kidney tissue analyzed by enzyme-linked immunosorbent assay (ELISA) is the standard method to detect the presence of Rs in salmon sampled in hatcheries. In this study, we tested the validity of using ELISA on kidney tissue collected from intact carcasses recovered on the spawning grounds to monitor BKD in naturally spawning populations by comparing ELISA optical density (OD) values from kidney tissue that was subjected to conditions that simulated decomposition in a carcass and collection during a spawning ground survey with samples freshly collected from salmon at a hatchery. Mean ELISA OD levels were 1.060 for the samples prepared by the normal preparation and 1.115 for samples prepared by simulating spawning ground survey collection. There was no significant difference in mean ELISA OD between the 2 sample preparations and the relationship did not significantly differ from 1:1 (slope = 0.946). This demonstrates that BKD prevalence in natural populations can be monitored using ELISA conducted on samples from intact carcasses recovered on spawning ground surveys. This will be an important tool for monitoring the effect of hatchery supplementation on naturally spawning salmon populations. PMID:17972755

  6. The Use of Chicken Igy in a Double Antibody Sandwich Elisa for the Quantification of Melittin in Bee Venom and Bee Venom Melittin Content in Cosmetics

    OpenAIRE

    Suh Lindsey Y. K.; Kartoon Tayabaa; Gujral Naiyana; Yoon Youngmee; Suh Joo Won; Sunwoo Hoon

    2015-01-01

    Two enzyme-linked immunosorbent assay (ELISA) - based detection systems: indirect competitive ELISA and biotinylated double antibody sandwich ELISA (DAS-ELISA) were developed to determine the melittin concentration in honeybee (Apis mellifera) venom and the melittin concentration in cosmetics which contain bee venom. The indirect competitive ELISA employed chicken anti-melittin IgY. The biotinylated DAS-ELISA employed anti-melittin monoclonal antibody (MAb) and biotinylated anti-melittin IgY....

  7. Caracterización molecular de la cadena gama común y Jak3 en un individuo afectado con inmunodeficiencia severa combinada

    Directory of Open Access Journals (Sweden)

    Pablo Javier Patiño Grajales

    2001-04-01

    Full Text Available

    La Inmunodeficiencia Severa Combinada (IDSC es una enfermedad
    de origen genético, que se puede heredar de forma autosómica
    recesiva o ligada al cromosoma X. La IDSC se caracteriza por un
    defecto en el número y la diferenciación de los linfocitos T y NK. Los
    individuos afectados desarrollan diarrea crónica, infecciones persistentes y severas como neumonía, septicemia e infecciones fúngicas.
    Estos pacientes presentan retardo en el crecimiento y pueden morir a
    temprana edad si no se realiza una terapia de corrección genética o un
    trasplante de células hematopoyéticas. Las mutaciones responsables
    de la IDSC comprometen principalmente el gen de la cadena gama
    común (γc y la proteína Jak3 que son proteínas fundamentales en la
    transducción de señales de los receptores para varias citoquinas esenciales en la diferenciación y activación de células del sistema inmune, las cuales incluyen IL-2, IL-4, IL-7, IL-9 e IL-15 (1,2.

     

     

  8. MODELIZACIÓN DE LOS INDICADORES DE GESTIÓN EN LA CADENA DE SUMINISTRO. UNA VISIÓN SISTÉMICA

    Directory of Open Access Journals (Sweden)

    MARTÍN DARIO ARANGO SERNA

    2008-01-01

    Full Text Available El modelo que describe este artículo, pretende medir, analizar, evaluar y plantear un mejoramiento de los procesos administrativos, identificando las diferentes variables que intervienen en la gestión de la cadena de suministro centrándose en el subsistema de producción. Este trabajo hace parte de una investigación empírica que se viene desarrollando en el sector textil. El modelo muestra de forma empírica las relaciones que existen entre, las funciones internas de la empresa en el área de la producción y su habilidad para la creación de indicadores de gestión de la producción y la dinámica empresarial como camino al mejoramiento e innovación de procesos. Finalmente, se muestran algunas conclusiones relevantes obtenidas en las dos primeras fases de la investigación empírica, ejecutadas hasta el momento.

  9. Development, standardisation and validation of ELISA methods to improve the control of trypanosomosis

    International Nuclear Information System (INIS)

    During the period from 1995 to 2000, comprehensive laboratory and field studies on enzyme-linked immunosorbent assay (ELISA) methods for detection of trypanosomal antibodies and antigens were undertaken to improve the proficiency of diagnostic laboratories involved in control of trypanosomosis in the tropics. The work was initiated by the FAO/IAEA through the Coordinated Research Programme D3.20.13 and undertaken in close collaboration with Research Agreement Holders and Research Contract Holders. Initially, the CRP facilitated the field evaluation of three direct sandwich antigen detection ELISAs based on monoclonal antibodies. Diagnostic laboratories were supported with ELISA equipment, disposables, and training. ELISA reagents were produced in sufficient quantities and distributed in a standardised kit format. As a result of the laboratory and field evaluation studies, the assays were found unreliable for trypanosomosis control and rejected for routine use in diagnostic laboratories. At that time, no standardised ELISA system was available for trypanosomosis that was considered suitable for distribution and use under tropical conditions. Through the CRP, a new generation of standardised antibody ELISAs were developed and established using a novel approach, namely the use of antigen-precoated ELISA plates. In addition, the potential of native and denatured trypanosomal antigens as diagnostic candidates was examined. In-house and field evaluation studies in the tropics demonstrated that a reasonable robustness with an acceptable diagnostic assay proficiency was achieved by means of utilising plates precoated with denatured antigens. Moreover, a data charting method for continuous monitoring of the operational performance of the ELISAs was developed and established. It was routinely used as remote control and follow up tool saving the need for costly expert missions to the diagnostic laboratories during the assay validation period. In parallel, preliminary studies

  10. Diagnosis of viral diseases using ELISA techniques: Current status and future prospects

    International Nuclear Information System (INIS)

    Use of enzyme-linked immunosorbent assays (ELISAs) for the detection of virus or anti-viral antibody has now become commonplace in diagnostic laboratories. These assays offer considerable advantages to the virologist over previously used tests because of their simplicity, lack of tissue culture requirements and ease of standardization. The direct ELISA is now seldom employed and indirect ELISAs using sandwich and competition procedures enable the versatility of ELISA to be fully exploited. Improvements in test procedures and reagents continue to increase the sensitivity and speed of the assays and utilization of monoclonal antibodies has resulted in greatly improved specificity with their use in competition assays, reducing the need for antigen purity. The versatility of new enzyme systems, with their increased sensitivity, improved conjugation and more distinctive colour changes, further increases the flexibility of this test system. In assay development and standardization use of sophisticated laboratory equipment is essential. The automatic ELISA microplate reader and high speed centrifuge are necessary tools for the correct establishment of a diagnostic ELISA. Once this step is completed standardized reagents are required in only small amounts and the test can be easily adapted for use in simply equipped laboratories using by-eye reading. This system of virus diagnosis has immense potential in the developing world. The cost and supply of reagents, difficulties of maintaining tissue culture and the complexity of equipment for other techniques for virus diagnosis are problems that have resulted in limited achievements in this area. ELISA offers a solution to most of these problems and perhaps its greatest prospect lies in the development of assays specifically designed for use in developing countries. (author)

  11. Validation of a phospholipase A2 receptor antibody ELISA in an Australian cohort with membranous glomerulonephritis.

    Science.gov (United States)

    Ong, Lawrence; Silvestrini, Roger; Chapman, Jeremy; Fulcher, David A; Lin, Ming Wei

    2016-04-01

    A commercial PLA2R Ab ELISA was validated by examining its ability to distinguish primary from secondary membranous nephropathy, correlating results with clinical markers of disease activity, and comparing its performance with an indirect immunofluorescence test (IIFT). PLA2R Ab levels were measured in 77 patients with biopsy proven membranous nephropathy, divided into either idiopathic (n = 61) or secondary groups (n = 6). In the idiopathic group, measures of contemporaneous disease activity (proteinuria, serum creatinine) were compared between seropositive and seronegative subjects. ELISA values were then compared with semi-quantitative results from an IIFT using PLA2R transfected HEK293 cells as substrate. The PLA2R Ab ELISA was positive in only 15 of 61 (25%) patients with idiopathic membranous nephropathy (IMN), but there was a significant negative relationship with time since diagnosis. Thus, in a subgroup of patients diagnosed within 6 months of analysis, the sensitivity was 6/15 (55%), rising to 6/8 (75%) in those recently-diagnosed patients who had not been treated. In the entire cohort, there was a significant positive correlation between ELISA values and degree of proteinuria, but our analysis did not control for variation of both variables with time. The PLA2R Ab ELISA also showed very high agreement with IIFT (96%). Therefore, the PLA2R Ab ELISA is a highly specific test for distinguishing primary from secondary membranous nephropathy that is most sensitive in newly diagnosed patients who have not received immunosuppression. Antibody levels correlated with degree of proteinuria, but this relationship was not shown to be independent of time. Both IIFT and ELISA platforms performed comparably. PMID:27020499

  12. Amplified ELISA to detect autoantibodies to N-glycolyl-GM3 ganglioside.

    Science.gov (United States)

    Iznaga, N; Carr, A; Fernández, L E; Solozabal, J; Núñez, G; Perdomo, Y; Morales, A

    1996-01-01

    Numerous immunochemical methods are now available for the detection of antibodies to gangliosides. An amplified ELISA method for detection of autoantibodies to NGcGM3 ganglioside in the sera of patients with various type of renal diseases was developed. IgM antibodies were found in 39 out of 53 sera of patients using 30 normal healthy blood donor as a negative control. For human IgG conjugate no reactivity to NGcGM3 was seen in the sera. Positive ELISA results were confirmed by TLC-immunostaining using GM3, NGcGM3, NGcGM2 and Standard bovine gangliosides (GM1, GD1a, GD1b and GT1b). All sera were also assayed for reactivity with GM3 in ELISA to determine the line specificity of these antibodies. Based on these results, a protocol for a sensitive and reproducible amplification ELISA system for serum anti-NGcGM3 antibodies in patients with renal or other diseases is presented. The ELISA method described here in appear to be useful adjunt to measure antiNGcGM3 antibodies in sera of patients with various type of renal or other diseases. PMID:16296265

  13. An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus

    Directory of Open Access Journals (Sweden)

    Åkerstedt J

    2002-12-01

    Full Text Available Infection with the intracellular microsporidium Encephalitozoon cuniculi can cause serious disease, encephalitozoonosis, in the blue fox (Alopex lagopus. The disease diagnosis is based on clinical signs and pathological findings, and detection of E. cuniculi or circulating antibodies directed against the parasite. Indirect immunofluorescence (IFAT and carbon immunoassay (CIA are the most commonly used serological methods for diagnosis in this species. In the present study, an indirect ELISA (enzyme linked immunosorbent assay was established and evaluated against IFAT by testing of 205 field samples from blue foxes. There was high agreement between the results of the ELISA and CIA (κ = 0.99, and the ELISA and IFAT (κ = 0.958. There was no significant statistical difference between the tests (p > 0.05. It was concluded that the ELISA could be used to identify seropositive farmed blue foxes. The advantage of the ELISA lies in the potential of screening large numbers of animals with the goal of eradicating E. cuniculi infection in the farms.

  14. Development of an Indirect ELISA for Serological Diagnosis of Bovine herpesvirus 5

    Science.gov (United States)

    Campos, Fabrício S.; da Rosa, Matheus C.; Finger, Paula F.; de Oliveira, Patricia D.; Conceição, Fabricio R.; Fischer, Geferson; Roehe, Paulo M.; Leite, Fábio P. L.

    2016-01-01

    Bovine herpesviruses 1 and 5 (BoHV-1 and BoHV-5) are economically important pathogens, associated with a variety of clinical syndromes, including respiratory and genital disease, reproductive failure and meningoencephalitis. The standard serological assay to diagnose BoHV-1 and BoHV-5 infections is the virus neutralization test (VNT), a time consuming procedure that requires manipulation of infectious virus. In the present study a highly sensitive and specific single dilution indirect ELISA was developed using recombinant glycoprotein D from BoHV-5 as antigen (rgD5ELISA). Bovine serum samples (n = 450) were screened by VNT against BoHV-5a and by rgD5ELISA. Compared with the VNT, the rgD5ELISA demonstrated accuracy of 99.8%, with 100% sensitivity, 96.7% specificity and coefficient of agreement between the tests of 0.954. The rgD5ELISA described here shows excellent agreement with the VNT and is shown to be a simple, convenient, specific and highly sensitive virus-free assay for detection of serum antibodies to BoHV-5. PMID:26866923

  15. Development of an Indirect ELISA for Serological Diagnosis of Bovine herpesvirus 5.

    Directory of Open Access Journals (Sweden)

    Luana A Dummer

    Full Text Available Bovine herpesviruses 1 and 5 (BoHV-1 and BoHV-5 are economically important pathogens, associated with a variety of clinical syndromes, including respiratory and genital disease, reproductive failure and meningoencephalitis. The standard serological assay to diagnose BoHV-1 and BoHV-5 infections is the virus neutralization test (VNT, a time consuming procedure that requires manipulation of infectious virus. In the present study a highly sensitive and specific single dilution indirect ELISA was developed using recombinant glycoprotein D from BoHV-5 as antigen (rgD5ELISA. Bovine serum samples (n = 450 were screened by VNT against BoHV-5a and by rgD5ELISA. Compared with the VNT, the rgD5ELISA demonstrated accuracy of 99.8%, with 100% sensitivity, 96.7% specificity and coefficient of agreement between the tests of 0.954. The rgD5ELISA described here shows excellent agreement with the VNT and is shown to be a simple, convenient, specific and highly sensitive virus-free assay for detection of serum antibodies to BoHV-5.

  16. Nanospherical Brush as Catalase Container for Enhancing the Detection Sensitivity of Competitive Plasmonic ELISA.

    Science.gov (United States)

    Huang, Xiaolin; Chen, Rui; Xu, Hengyi; Lai, Weihua; Xiong, Yonghua

    2016-02-01

    Plasmonic enzyme-linked immunosorbent assay (pELISA) based on catalase (CAT)-mediated gold nanoparticle growth shows great potential for the determination of disease-related biomarkers at ultralow concentrations by using sandwich formats. However, the relatively low sensitivity of this strategy using competitive formats limits its adoption for hapten detection. Herein, we present an improved competitive pELISA for ultrasensitive detection of ochratoxin A (OTA), where silica nanoparticles carrying poly(acrylic acid) brushes (SiO2@PAA) were used to decrease the affinity of competing antigens to anti-OTA monoclonal antibodies and amplify the signal as a "CAT container" (SiO2@PAA@CAT). The developed competitive pELISA exhibits extremely high sensitivity for OTA with detection limits of 10(-18) and 5 × 10(-20) g/mL by the naked eye and microplate reader, respectively. These values are at least 7 orders of magnitude lower than that of competitive CAT-based pELISA (10(-11) g/mL by the naked eye) and 8 orders of magnitude lower than that of horseradish peroxidase-based conventional ELISA (10(-11) g/mL by the microplate reader), respectively. Reliability and robustness of the proposed method were evaluated using actual agricultural products and human serum samples. This study demonstrated the potential of this modified method in practical applications involving the ultrasensitive detection of mycotoxins or other haptens. PMID:26719076

  17. His-tag ELISA for the detection of humoral tumor-specific immunity

    Directory of Open Access Journals (Sweden)

    Disis Mary L

    2008-05-01

    Full Text Available Abstract Background The application of high throughput molecular techniques such as SEREX are resulting in the identification of a multitude of tumor associated antigens. As newly identified antigens are incorporated into a variety of clinical trials, standardization of immunologic monitoring methods becomes increasingly important. We questioned whether mammalian cell expression of a histadine-linked human protein could be used to produce antigen suitable for detecting tumor-specific humoral immunity and whether such an assay could be amenable to standardization for clinical use. Methods We designed a his-tagged capture ELISA based on lysate from genetically engineered CHO cells for detection of antibodies to insulin-like growth factor binding protein 2, a novel tumor antigen. We performed technical and preliminary clinical validation studies, including comparison to a standard indirect ELISA based on commercially prepared recombinant antigen. Results The his-tagged capture ELISA could be standardized. Precision experiments resulted in CVs 2 values of 0.99. In comparison to Western blot analysis, his-tag and indirect ELISA accurately identified 88% and 93% of samples, respectively. Sample concordance between capture and indirect assays was highly significant (p = 0.003. Furthermore, significantly greater levels of IGFBP-2 antibody immunity were found in cancer patients compared to normal controls (p = 0.008. Conclusion A genetically engineered cell lysate based ELISA can be amenable to standardization and can detect increased levels of antibody immunity to tumor-associated antigen in cancer patients compared to non tumor-bearing healthy controls.

  18. Performance of an RT-nested PCR ELISA for detection of Newcastle disease virus.

    Science.gov (United States)

    Kho, C L; Mohd-Azmi, M L; Arshad, S S; Yusoff, K

    2000-04-01

    A sensitive and specific RT-nested PCR coupled with an ELISA detection system for detecting Newcastle disease virus is described. Two nested pairs of primer which were highly specific to all the three different pathotypes of NDV were designed from the consensus fusion gene sequence. No cross-reactions with other avian infectious agents such as infectious bronchitis virus, infectious bursal disease virus, influenza virus, and fowl pox virus were observed. Based on agarose electrophoresis detection, the RT-nested PCR was about 100 times more sensitive compared to that of a non-nested RT-PCR. To facilitate the detection of the PCR product, an ELISA detection method was then developed to detect the amplified PCR products and it was shown to be ten times more sensitive than gel electrophoresis. The efficacy of the nested PCR-ELISA was also compared with the conventional NDV detection method (HA test) and non-nested RT-PCR by testing against a total of 35 tissue specimens collected from ND-symptomatic chickens. The RT-nested PCR ELISA found NDV positive in 21 (60%) tissue specimens, while only eight (22.9%) and two (5.7%) out of 35 tissue specimens were tested NDV positive by both the non-nested RT-PCR and conventional HA test, respectively. Due to its high sensitivity for the detection of NDV from tissue specimens, this PCR-ELISA based diagnostic test may be useful for screening large number of samples. PMID:10713378

  19. Designing and evaluation of Dot-ELISA for diagnosis of Fasciola infection in cattle

    Directory of Open Access Journals (Sweden)

    Jafar Arjmand

    2014-06-01

    Full Text Available Fasciolosis is a disease caused by liver fluck of the genus of fasciola. Diagnosis of fasciolosis has been challenging for a long period due to low sensitivity of the coprological diagnostic method. In this study, an in-house Dot-ELISA method; using excretion–secretory (ES Ag and Crude (Cr Ag antigens of fasciola was described for diagnosis of fasciolosis in cattle. For this purpose, the sera specimens of slaughtered cattle were taken and examined for fasciola infection. Sera from two groups of cattle, one infected with fasciola (n = 60 and the other non-infected with fasciola (n = 60, were used in the Dot- ELISA test. All sera were tested and evaluated. Except specificity, other parameters such as, sensitivity, accuracy, positive and negative predictive values of Dot- ELISA with ES Ag were better than those of Dot- ELISA with Cr Ag. In conclusion, excretory–secretory antigen dependent Dot-ELISA can be used as a reliable sero-diagnostic test for fasciola infection in cattle.

  20. A Sensitive Competitive ELISA for Determination of Biotin in Transformed Yeast Culture Media

    Institute of Scientific and Technical Information of China (English)

    YANGHong

    2003-01-01

    Aim To develop a sensitive competitive ELISA for the determination of biotin in transformed yeast culture media.Methods The ELISA plate was firstly coated with Mycoplasma hyopneumoniae, and then successively incubated with rabbit ami-Mycoplasma hyopneumoniae serum and goat anti-rabbit IgG-biotin to form the solid biotin, which competed with the biotin in the solution (standard or sample) for the limited streptavidin-horse radish peroxidase conjugate. The standard calibration curve for biotin analysis was constructed in the range of 50-2000ng·L-1. Results The detection limit for biotin was found to be 83 ng·L-1 , which waa about 1000 times lower than the lowest determination concenlration in the reported ELISA for biotin analysis. The relative standard deviations for the spiked samples at biotin concerarations of 200 ng·L-1, 500 ng·L-1 , and 1000 ng·L-1 were 24.87%, 6.15%, and 7.86%, respectively, with the average recovery of 101.13%. The wild yeast and its sixty-three transformed yeast culture media were applied to the developed ELISA for the determination of biotin. It was found that the biotin concentrations in more than 85 % of the tested samples were enhanced with different increase factors after transformation. Conclusion Utilization of Mycoplasma hyopnetunoniae as the coating protein improves the precision and accmacy oftbe ELISA assay, which might be used for the biotin assay in other media.

  1. Multicolor ELISA based on alkaline phosphatase-triggered growth of Au nanorods.

    Science.gov (United States)

    Li, Yanyan; Ma, Xiaoming; Xu, Zhengming; Liu, Meihua; Lin, Zhenyu; Qiu, Bin; Guo, Longhua; Chen, Guonan

    2016-05-10

    Seed-mediated synthesis of gold nanorods (AuNRs) has been widely used for diverse applications in the past decade. In this work, this synthetic process is demonstrated for multicolor biosensing for the first time. Our investigation reveals that ascorbic acid acts as a key factor to mediate the growth of AuNRs. This phenomenon is incorporated into the alkaline phosphatase (ALP)-enzyme-linked immunosorbent assay (ELISA) system based on the fact that ALP can catalyze the conversion of ascorbic acid-phosphate into ascorbic acid with high efficiency. This allows us to develop a multicolor ELISA approach for sensitive detection of disease biomarkers with the naked eye. We show the proof-of-concept multicolor ELISA for the detection of prostate-specific antigen (PSA) in human serum. The results show that different colors are presented in response to different concentrations of PSA, and a detection limit of 3 × 10(-15) g mL(-1) in human serum was achieved. The proposed multicolor ELISA could be a good supplement to conventional ELISA for POC diagnostics. PMID:27050384

  2. Detergent pretreatment of solid phase globular proteins in ELISA`s. Enhanced antigenicity and subsequent sensitivity. Final report, September 1989-September 1991

    Energy Technology Data Exchange (ETDEWEB)

    Blanchard, G.C.; Bouhmadouche, M.; Williamson, M.L.

    1994-10-01

    Methods for pretreatment and rejuvenation of preimmobilized globular proteins used in immunodiagnostics were investigated using reagents routinely used in ELISA`s. Rabbit and goat gamma globulins, functioning as antigens, and antibodies on non-covalent, and covalent solid surfaces, were monitored for detergent mediated desorption, denaturation, non-specific binding and altered antigenicity. The results from fourteen commercially supplied polyvinyl- and polystyrene-derivatized microtiter plates coated with antibody or antigenic lgG were compared with commercial microtiter diagnostic plates with preimmobilized lgG. Wash solutions had no effect on immobilized gamma globulins when the solid phase protein functioned as an antibody on covalent or noncovalent surfaces. In addition to tween 20 removing up to 50% of noncovalently bound protein additional binding sites are apparently exposed on solid phase antigens, evident by an increase in signal, which cannot be explained by nonspecific binding. However, no increase in signal was evident when antigen was preimmobilized covalently. The role of between 20 and other reagent components in ELISA-based assays are explored. The screening of noncovalent preimmobilized antigen coated surfaces prior to use for deteraent mediated enhancement is suggested.

  3. Development of sandwich ELISAs for the detection of aromatic diisocyanate adducts.

    Science.gov (United States)

    Lemons, Angela R; Bledsoe, Toni A; Siegel, Paul D; Beezhold, Donald H; Green, Brett J

    2013-11-29

    Diisocyanates (dNCOs) are highly reactive low molecular weight chemicals commonly used in the manufacturing industry. Occupational exposures to dNCOs have been shown to elicit allergic sensitization and occupational asthma. Among the most commonly used dNCOs in industry are the aromatic dNCOs, toluene diisocyanate (TDI) and methylene diphenyl diisocyanate (MDI). This study aimed to develop enzyme linked immunosorbent assays (ELISA) utilizing aromatic dNCO-specific monoclonal antibodies (mAbs) for the detection of aromatic dNCO adducts. Two sandwich ELISAs were developed. The first sandwich ELISA utilized mAb 60G2 along with an anti-human serum albumin (HSA) polyclonal antibody. This assay detected MDI-, 2,4- and 2,6-TDI-HSA adducts with limits of detection (LOD) of 2.67, characterization of aromatic dNCO adducts as well as in biomonitoring occupational and environmental dNCO exposures. PMID:24012971

  4. Follow-up of neurocysticercosis patients after treatment using an antigen detection ELISA

    Directory of Open Access Journals (Sweden)

    Nguekam

    2003-03-01

    Full Text Available Seven patients with active neurocysticercosis (NCC received an eight days treatment with albendazole and were followed up using computed tomography (CT-scan and a monoclonal antibody based ELISA for the detection of circulating antigen (Ag-ELISA. Only three patients were cured as was shown by CT-scan and by the disappearance of circulating antigens one month after treatment. After a second course of albendazole therapy, two other patients became seronegative. CT-scan showed the disappearance of viable cysts in all persons who became seronegative whereas patients who were not cured remained seropositive. These preliminary results show that this Ag-ELISA is a promising technique for monitoring the success of treatment of NCC patients because of the excellent correlation between the presence of circulating antigens and of viable brain cysts.

  5. Investigation of Entamoeba histolytica in stool specimens by ELISA during a year

    Directory of Open Access Journals (Sweden)

    Mehmet Sinan Dal

    2011-03-01

    Full Text Available The aim of this study was to investigate Entamoebahistolytica in the stool samples that have beensent to microbiology laboratory by ELISA method.Materials and methods: This study was performed on800 stool specimens belonging to different age groupsand different patients sent to Microbiology Laboratory ofa State Hospital between January 2009 and December2009.Results: In this study Entamoeba histolytica/dispar cystsand/or trophozoites were observed in 31 (3.9% out of atotal of 800 stool specimens which were examined bynative-lugol. Entamoeba histolytica specific antigen wasinvestigated by ELISA in all stool specimens. Entamoebahistolytica specific antigen was detected in 12 (1.5% ofthese stool specimens. The diagnosis of amoebiasis forthe patients whose ELISA tests were “positive” and appropriatetherapy was begun.Conclusion: Entamoeba histolytica in the stool samplesshould be investigated and unnecessary treatmentsshould not given to patients. J Clin Exp Invest 2011; 2(1:50-54

  6. Comparison of three commercial fecal calprotectin ELISA test kits used in patients with Inflammatory Bowel Disease

    DEFF Research Database (Denmark)

    Mirsepasi-Lauridsen, Hengameh Chloé; Bachmann Holmetoft, Ulla; Ingdam Halkjær, Sofie;

    2016-01-01

    OBJECTIVE: Fecal calprotectin is a noninvasive marker of intestinal inflammation used to distinguish between functional and organic bowel diseases and to evaluate disease activity among patients with Inflammatory Bowel Disease (IBD). The goal of this study was to compare three different ELISA tests...... measuring calprotectin in their accuracy to detect IBD and to distinguish between IBD patients with active or inactive disease. MATERIAL AND METHODS: This study includes in total 148 fecal samples, 96 from patients with a previously confirmed IBD diagnosis and 52 from healthy controls, aged from 25 to 86...... tests (EK-CAL, CALPRO and HK325) were performed on fecal specimens and results compared. RESULTS: The CALPRO calprotectin ELISA test was shown to have the best specificity of 96% compared to the HK325 and the EK-CAL calprotectin ELISA tests with 28% specificity and 74% specificity, respectively. A...

  7. IgG Adhesion on Hydrophobic Surfaces: Theory, Modelling, and Application to ELISA

    CERN Document Server

    de Thier, P

    2016-01-01

    Enzyme-Linked ImmunoSorbent Assays (ELISA) are a range of widely used analytical methods whose implementation requires to build antibodies (IgG) thin films onto surfaces predominantly made of polystyrene. The high hydrophobicity of polystyrene ensures a spontaneous and strong adhesion of proteins allowing to easily build IgG monolayers. Since the ELISA improvements definitely lie in the comprehension of physico-chemical mechanisms on which IgG immobilization on hydrophobic surfaces are relied, this work develops a theorization essay (thermodynamics of the so-called hydrophobic effect and of thin films building) emphasized by numerical modelling (random sequential additions model, i.e. RSA) and experimental estimations by atomic force microscopy (AFM) and ELISA. Keeping in mind the hydrophobic effect, thermodynamics (of irreversible processes) allows to explain why IgG adhesion on polystyrene occurs whereas numerical modelling approaches show a way of surface saturation leading to promote IgG orientations expe...

  8. Prevalence of Bovine Brucellosis in Organized Dairy Farms, Using Milk ELISA, in Quetta City, Balochistan, Pakistan

    Directory of Open Access Journals (Sweden)

    Muhammad Shafee

    2011-01-01

    Full Text Available A total of 200 milk samples from cattle (=86 and buffalo (=114 were evaluated using milk ring test (MRT and indirect enzyme-linked immunosorbent assay (i-ELISA. The overall prevalence was found to be 3% and 8.5% in cattle and buffaloes using MRT and i-ELISA, respectively. The prevalence was 4.6% and 1.7% in cattle and buffalo using MRT, respectively, while i-ELISA exhibited 20% and 0% in cattle and buffalo, respectively. The prevalence was higher in government dairy farm, compared to privately owned dairy farm. This paper points out an alarming situation in the target area with respect to the public health significance.

  9. Field trial of a brucellosis competitive enzyme linked immunoabsorbent assay (ELISA)

    International Nuclear Information System (INIS)

    The purpose of this study was to evaluate the performance of a competitive ELISA system for the diagnosis of bovine brucellosis in comparison to conventional aerological tests routinely used in Argentina. A total of 2.500 serum samples, comprising Brucella-free herds, vaccinated cattle and naturally infected animals, was tested by the following tests: buffered plate agglutination, Rose Bengal, 2-mercaptoethanol, complement fixation, and indirect and competitive ELISAs. Specificity and relative sensitivity at each test were determined. The competitive ELISA was considered suitable for detection of vaccinated animals and had higher specificity than the other tests. The results point to the potential use of the test as a complementary assay in the brucellosis control programme in Argentina. (author)

  10. Development and use of ELISA in the control of foot-and-mouth disease

    International Nuclear Information System (INIS)

    Foot-and-mouth disease (FMD) is a highly contagious and economically devastating disease that requires laboratory investigation for definitive diagnosis. Highly specific and sensitive assays are needed to obtain a rapid result and so optimize the efficiency of FMD diagnosis and formulation of appropriate control measures. The development of the enzyme linked immunosorbent assay (ELISA) has produced marked improvements in the diagnosis of FMD. An indirect sandwich ELISA has replaced the complement fixation test for FMDV virus antigen detection and a liquid phase blocking ELISA (LPBE) has replaced the virus neutralization test for the detection of serum antibodies. The LPBE is also employed to test the suitability of available vaccine strains for use in current outbreaks. The assays mainly use polyclonal antisera, but monoclonal antibodies have brought greater precision to virus strain characterization studies and have the potential to replace polyclonal sera in other assays. The provision of ELISA kits enables national FMD laboratories in developing countries to carry out their own assays in the first instance and allows effective and more immediate decisions to be made on control. At present, there are no reliable mass serum screen laboratory tests (i.e. ELISA) that can distinguish vaccinated from infected and carrier from non-carrier animals. These groups of animals have to be considered potential carriers of FMD virus and a source of new disease and thus need to be excluded from internal and international trade. ELISAs employing reagents to bioengineered non-structural proteins of FMD virus hold considerable promise for the discrimination of naive, convalescent and vaccinated animals, and work is in progress for their refinement. (author)

  11. False positive detection of peanut residue in liquid caramel coloring using commercial ELISA kits.

    Science.gov (United States)

    Stelk, T; Niemann, L; Lambrecht, D M; Baumert, J L; Taylor, S L

    2013-07-01

    Initial food industry testing in our laboratory using enzyme-linked immunosorbent assay (ELISA) methods indicated that the darkest caramel color (class IV) unexpectedly contained traces of peanut protein, a potential undeclared allergen issue. Caramel production centers on the heating of sugars, often glucose, under controlled heat and chemical processing conditions with other ingredients including ammonia, sulfite, and/or alkali salts. These ingredients should not contain any traces of peanut residue. We sought to determine the reliability of commercially available peanut allergen ELISA methods for detection of apparent peanut residue in caramel coloring. Caramel color samples of classes I, II, III, and IV were obtained from 2 commercial suppliers and tested using 6 commercially available quantitative and qualitative peanut ELISA kits. Five lots of class IV caramel color were spiked with a known concentration of peanut protein from light roasted peanut flour to assess recovery of peanut residue using a spike and recovery protocol with either 15 ppm or 100 ppm peanut protein on a kit-specific basis. A false positive detection of peanut protein was found in class IV caramel colors with a range of 1.2 to 17.6 parts per million recovered in both spiked and unspiked liquid caramel color samples. ELISA kit spike/recovery results indicate that false negative results might also be obtained if peanut contamination were ever to actually exist in class IV caramel color. Manufacturers of peanut-free products often test all ingredients for peanut allergen residues using commercial ELISA kits. ELISA methods are not reliable for the detection of peanut in class IV caramel ingredients and their use is not recommended with this matrix. PMID:23647653

  12. Evaluation of dengue NS1 antigen rapid tests and ELISA kits using clinical samples.

    Directory of Open Access Journals (Sweden)

    Subhamoy Pal

    Full Text Available Early diagnosis of dengue virus (DENV infection can improve clinical outcomes by ensuring close follow-up, initiating appropriate supportive therapies and raising awareness to the potential of hemorrhage or shock. Non-structural glycoprotein-1 (NS1 has proven to be a useful biomarker for early diagnosis of dengue. A number of rapid diagnostic tests (RDTs and enzyme-linked immunosorbent assays (ELISAs targeting NS1 antigen (Ag are now commercially available. Here we evaluated these tests using a well-characterized panel of clinical samples to determine their effectiveness for early diagnosis.Retrospective samples from South America were used to evaluate the following tests: (i "Dengue NS1 Ag STRIP" and (ii "Platelia Dengue NS1 Ag ELISA" (Bio-Rad, France, (iii "Dengue NS1 Detect Rapid Test (1st Generation" and (iv "DENV Detect NS1 ELISA" (InBios International, United States, (v "Panbio Dengue Early Rapid (1st generation" (vi "Panbio Dengue Early ELISA (2nd generation" and (vii "SD Bioline Dengue NS1 Ag Rapid Test" (Alere, United States. Overall, the sensitivity of the RDTs ranged from 71.9%-79.1% while the sensitivity of the ELISAs varied between 85.6-95.9%, using virus isolation as the reference method. Most tests had lower sensitivity for DENV-4 relative to the other three serotypes, were less sensitive in detecting secondary infections, and appeared to be most sensitive on Day 3-4 post symptom onset. The specificity of all evaluated tests ranged from 95%-100%.ELISAs had greater overall sensitivity than RDTs. In conjunction with other parameters, the performance data can help determine which dengue diagnostics should be used during the first few days of illness, when the patients are most likely to present to a clinic seeking care.

  13. APLICACIÓN DE LA TOMA DE DECISIONES MULTICRITERIO EN LA CADENA DE CORTE, ALZA Y TIRO DE LA CAÑA DE AZÚCAR

    Directory of Open Access Journals (Sweden)

    Fernando Marrero Delgado

    2001-01-01

    Full Text Available

    La situación actual en que se encuentra la economía cubana hace necesario que se eleve su eficacia a fin de contribuir a su recuperación. Al ser esta una de las ramas principales, es imprescindible aplicar técnicas que permitan asegurar un funcionamiento óptimo de cada una de sus etapas y áreas, siendo la cadena de corte, alza y transportación de la caña de azúcar el eslabón que vincula a la agricultura con la industria y donde se incurre en una serie de costos que afectan decisivamente el costo total del proceso de obtención de azúcar; por lo que es importante evaluar la misma con varios criterios de desempeño. Por esta razón, es imprescindible el uso de la óptica multicriterio para la toma de decisiones en esta área y precisamente aquí radica el objetivo del presente trabajo, donde se presenta un modelo multimeta para evaluar el desenvolvimiento de la transportación de la caña desde el campo hasta la industria

    .

  14. Development and validation of a competitive ELISA kit for the serological diagnosis of ovine, caprine and bovine brucellosis.

    Science.gov (United States)

    Portanti, O; Tittarelli, M; Di Febo, T; Luciani, M; Mercante, M T; Conte, A; Lelli, R

    2006-12-01

    A competitive ELISA (Brucella-Ab c-ELISA) was standardized and validated for the detection of Brucella antibodies in cattle, sheep and goat sera using a monoclonal antibody (MAb 4B5A) produced against Brucella melitensis biotype 2. The specificity and sensitivity of the assay were 100% to a 67.5% cut-off point (B/Bo%). When compared with an indirect ELISA, the Brucella-Ab c-ELISA did not demonstrate cross-reactions when testing positive sera for antibodies to some Enterobacteriaceae. A comparison was made between the Brucella-Ab c-ELISA and the complement fixation and Rose Bengal tests. Results demonstrated that the Brucella-Ab c-ELISA is a valuable tool for the serological diagnosis of bovine and ovine/caprine brucellosis. PMID:17123429

  15. Capture ELISA for IgM antibodies against Plasmodium falciparum glutamate rich protein

    DEFF Research Database (Denmark)

    Dziegiel, Morten Hanefeld; Borre, M B; Petersen, E;

    1992-01-01

    This report describes a novel mu chain capture ELISA for the detection of IgM antibodies against a Plasmodium falciparum antigen. A fragment of the 220 kDa P. falciparum glutamate rich protein containing amino acid residues 489-1271 was expressed in E. coli as a recombinant chimeric beta-galactos......This report describes a novel mu chain capture ELISA for the detection of IgM antibodies against a Plasmodium falciparum antigen. A fragment of the 220 kDa P. falciparum glutamate rich protein containing amino acid residues 489-1271 was expressed in E. coli as a recombinant chimeric beta...

  16. Extension of the four-parameter logistic model for ELISA to multianalyte analysis.

    Science.gov (United States)

    Jones, G; Wortberg, M; Kreissig, S B; Bunch, D S; Gee, S J; Hammock, B D; Rocke, D M

    1994-12-28

    The standard implementation of enzyme-linked immunosorbent assay (ELISA) for single analytes can lead to false conclusions if cross reacting compounds are present in the sample. This paper discusses the extension of the usual four-parameter logistic model for ELISA to the case of multiple cross-reacting analytes. The use of the extended model in multianalyte analysis (MELISA) is illustrated and compared with a more simplistic approach. Data on the analysis of a binary mixture of s-triazines suggests the superiority of the proposed model. This model is also suitable for other forms of immunoassay that use the four-parameter logistic curve. PMID:7822815

  17. Construction of an optical test-bed for eLISA

    OpenAIRE

    Lieser, M.; Fitzsimons, E; Isleif, K.S.; Killow, C; Perreur-Lloyd, M; Roberstson, D.; Schuster, S.; Trobs, M.; Veith, S.; Ward, H; Heinzel, G.; Danzmann, K.

    2016-01-01

    In the planned eLISA mission a key part of the system is the optical bench that holds the interferometers for reading out the inter-spacecraft distance and the test mass position. We report on ongoing technology development for the eLISA optical system like the back-link between the optical benches and the science interferometer where the local beam is interfered with the received beam from the distant spacecraft. The focus will be on a setup to investigate the tilt-to-pathlength coupling in ...

  18. Serodiagnosis of Rift Valley fever in African wildlife using a recombinant nucleocapsid-based indirect ELISA

    International Nuclear Information System (INIS)

    Antibodies to Rift Valley fever virus (RVFV) have been found in many wildlife species but their importance in the epidemiology of the disease during the inter-epidemic and epidemic periods, and including their possible specific role in the cryptic maintenance of the virus is not elucidated. A recently developed indirect ELISA (I-ELISA) based on recombinant nucleocapsid protein (rNp) of RVF virus was reported to have high analytical accuracy for the detection of IgG antibody in African buffalo sera. An indirect ELISA (I-ELISA) based on the recombinant nucleocapsid protein (rNp) of Rift Valley fever virus (RVFV) was evaluated for the detection of specific serum IgG antibody in African wildlife. Data sets derived from field-collected sera (n = 877) in Africa (antelopes = 529, black rhinoceros = 43, common zebra = 24, elephant = 73, giraffe = 81, grevy zebra = 78, warthog = 49) were categorized according to the results of a virus neutralization test. Dose response curves using different dilutions of sera known to be positive or negative in the virus neutralisation test had the expected analytical slope and the I-ELISA clearly differentiated between different levels of specific IgG antibody against RVFV in African wildlife. At cut-offs optimised by the two-graph receiver operating characteristics analysis, the diagnostic sensitivity of the I-ELISA was 100% and diagnostic specificity ranged from 99.8% to 100% while estimates for the Youden's index (J) and efficiency (Ef) ranged from 0.99 to 1 and from 99.7% to 100%, respectively. The rNp-based I-ELISA is highly accurate, safe, and offers a single assay format for rapid detection of IgG antibody to RVFV in sera of different wildlife species. This study confirm previous findings that the rNp-based I-ELISA accurately identifies sera with different concentrations of specific IgG antibodies to RVF virus, and compared to virus neutralization test it has very high diagnostic performance in various wildlife animal species. As a

  19. Validation and use of an ELISA kit for the diagnosis of Babesia bovis in Cuba

    International Nuclear Information System (INIS)

    Babesia bovis, the most important etiological agent causing bovine babesiosis, is widely distributed in Cuba and affects mainly adult cattle. A survey of the prevalence of the disease in cattle using an ELISA kit (FAO/IAEA) revealed that 34.2% of the animals between 6 and 18 months of age were positive to Babesia bovis, whereas 69.9% on the cattle older than 18 months were positive. Antibodies to Babesia bovis were detected in 96.9% of calves vaccinated with an attenuated Babesia bovis vaccine. A good correlation was found between the results of ELISA kit with those from indirect immunofluorescence and immunoperoxidase tests developed in Cuba. (author)

  20. Utilización de los test de Fluorescencia Polarizada (FP) y Elisa de Competencia (C-Elisa) en el diagnóstico de brucelosis de camélidos The use of polarized fluorescence assay (PF) and competitive ELISA test (C-Elisa) for the diagnosis of Brucellosis in South American camelids

    OpenAIRE

    Rojas, X.; Muñoz, S.; Otto, B.; B. Pérez; Nielsen, K.

    2004-01-01

    En Chile, como en muchos países de Latinoamérica, la brucelosis es una zoonosis presente, representando no sólo un factor de riesgo para las explotaciones pecuarias sino también para quienes laboran y hacen usufructo de ella, especialmente por el consumo de leche cruda y sus derivados. Como la Brucella afecta a diferentes especies de animales, el objetivo de este trabajo fue utilizar las técnicas de unión primaria, como son Fluorescencia Polarizada (FP) y Elisa de Competencia (C-Elisa), para ...

  1. Validation of the ELISA technique for diagnosis of trypanosomiasis in cattle in Uganda

    International Nuclear Information System (INIS)

    ELISA, developed in ILRAD for diagnosis of T. congolense, T. brucei and T. vivax in cattle, has not been validated in Uganda. This study was undertaken to validate the technique. Negative reference sera were collected from 44 cattle in Kapchorwa, a tsetse-free area. The cattle were free of the three trypanosome species T. congolense, T. brucei and T. vivax by the haematocrit buffy coat technique (BCT). But by ELISA, three were positive for T. vivax, one for both T. congolense and T. vivax and one for T. congolense. Sera were collected from the same 44 cattle 10 weeks later. The cattle were again free of T. congolense, T. brucei and T. vivax, both by BCT and by mouse inoculations. Two cattle out of 450 screened at a centre 5 km away had T. vivax by BCT. The ELISA results for the second set of sera were quite similar to the results obtained from the first set of sera. The calculated optical density (D) cut off point was 50 for both T. brucei and T. vivax, but it was 60 for T. congolense. Sera from 5 cattle which had T. theileri and two which had microfilaria were all negative for antigenaemia by ELISA. Positive reference sera were collected form 40 cattle in a high tsetse challenge area. Using the haematocrit buffy coat technique, 5 had T. vivax, two had T. brucei and one had T. congolense. Checked by ELISA for antigenaemia, only 4 cattle were free of all the three trypanosome species, T. congolense, T. vivax and T. brucei. All the 40 cattle were treated with Diminazene aceturate at the rate of 7 mg/kg body weight. Two weeks later, the ELISA test showed that 10 cattle were free of any antigenaemia. Those still positive for antigenaemia had lower OD readings. The ELISA technique is valid. It is much more sensitive compared to parasitological tests. It is specific since none of the 7 cattle with either T. theileri or microfilaria gave positive results by ELISA. The technique would be very useful for epizootiological studies. (author)

  2. Jatobal virus antigenic characterization by ELISA and neutralization test using EIA as indicator, on tissue culture

    Directory of Open Access Journals (Sweden)

    Luiz Tadeu M. Figueiredo

    1988-06-01

    Full Text Available A virus antigenic characterization methodology using an indirect method of antibody detection ELISA with virus-infected cultured cells as antigen and a micro virus neutralisation test using EIA (NT-EIA as an aid to reading were used for antigenic characterization of Jatobal (BeAn 423380. Jatobal virus was characterized as a Bunyaviridae, Bunyavirus genus, Simbu serogroup virus. ELISA using infected cultured cells as antigen is a sensitive and reliable method for identification of viruses and has many advantages over conventional antibody capture ELISA's and other tests: it eliminates solid phase coating with virus and laborious antigen preparation; it permits screening of large numbers of virus antisera faster and more easily than by CF, HAI, or plaque reduction NT. ELISA and NT using EIA as an aid to reading can be applicable to viruses which do not produce cytopathogenic effect. Both techniques are applicable to identification of viruses which grow in mosquito cells.A caracterização antigênica do vírus Jatobal (BeAn 423380 foi efetuada utilizando uma técnica de ELISA para deteccão de anticorpos que utiliza culturas celulares infectadas como antígeno e um micro teste de neutralização para vírus que utiliza o método imunoenzimático como auxiliar para a leitura dos resultados (NT-EIA. O vírus Jatobal foi caracterizado como um Bunyaviridae, gênero Bunyavirus, pertencente ao sorogrupo Simbu. A técnica de ELISA, utilizando culturas celulares infectadas como antígeno, trata-se de método sensível e confiável na identificação de agentes virais, possuindo muitas vantagens sobre ELISA convencionais e outros testes: elimina a preparação laboriosa de antígenos para o revestimento em fase sólida; permite que se teste de forma mais rápida e fácil que por CF, HAI e neutralização por redução de plaques um grande número de antisoros de vírus. ELISA e NT-EIA podem ser utilizados para a classificação de vírus que não produzem

  3. Análisis multinivel de cadenas de suministros: dos técnicas de resolución del efecto bullwhip || Supply Chain Multi-level Analysis:Two Bullwhip Dampening Approaches

    Directory of Open Access Journals (Sweden)

    Ciancimino, Elena

    2009-01-01

    Full Text Available El efecto bullwhip es un fenómeno causante de ineficiencias en la gestión de la cadena de suministros. El objetivo del presente artículo es analizar el impacto sobre el rendimiento de una cadena de suministro de dos de las técnicas más comunes de resolución del efecto bullwhip: la regla de pedido (S, R amortiguada y la adopción de prácticas de colaboración. Para ello, se adopta un sistema de métricas con el objetivo de evaluar los efectos sobre los diferentes eslabones de la cadena. Estos efectos se miden en términos de la estabilidad de la orden de pedido y de los inventarios, de la robustezdel sistema y de los beneficios para el cliente. Los resultados se analizan desde un punto de vista puramente técnico y desde el punto de vista de las implicaciones para las empresas. || The bullwhip effect is a phenomenon that causes inefficiencies in supply chains. The aim of this paper is to investigate on the different impact on theperformance of a supply chain of two bullwhip solving techniques: the (S, R smoothing replenishment policy and collaboration practices. Performance is evaluated through a comprehensive set of metrics, aimed at assessing the order rate stability, the inventory stability, the robustness of the system and the customer service level. Results are analysed both from a technical perspective and from the managerial point of view.

  4. La Gestión del Conocimiento en la Cadena de Suministro: Análisis de la Influencia del Contexto Organizativo Knowledge Management in the Supply Chain: Analysis of the Influence of the Organizational Context

    Directory of Open Access Journals (Sweden)

    Josep Capó-Vicedo

    2007-01-01

    Full Text Available El objetivo de este artículo es analizar que estructura o modelo organizativo es el más apropiado para que se dé una verdadera gestión del conocimiento en una cadena de suministro. Para ello se realiza una breve revisión de alguna de las aportaciones más significativas en la literatura existente sobre la relación entre la gestión del conocimiento y el contexto organizativo, así como sobre los requerimientos necesarios para gestionar el conocimiento en un contexto interorganizativo, como es el existente en una cadena de suministro. Del análisis realizado se concluye que la estructura organizativa conocida como Empresa Virtual es la más apropiada para gestionar el conocimiento en una cadena de suministro, por sus características intrínsecas de confianza mutua entre sus miembros, así como por el dinamismo en su creación y operaciónThe aim of this paper is to analyze which organizational structure or model is the most suitable so that a real knowledge management in a supply chain takes place. To achieve this objective, a brief revision of the existing literature related to the relationship between knowledge management and the organizational context and the necessary requirements to manage the knowledge in an inter-organizational context is done. From this analysis, it is concluded that the Virtual Enterprise is the most suitable organizational structure to manage knowledge in a supply chain, based on the intrinsic features of mutual confidence between its members and the dynamism in its creation and operation

  5. Determination of the Trypanosoma congolense and the Trypanosoma evansi antibodies detection ELISA for the diagnosis of surra in cattle in Thailand

    International Nuclear Information System (INIS)

    An IAEA Trypanosoma congolense ELISA kit and T.evansi NIAH/ELISA were used for the detection of T.evansi antibodies in 850 dairy cattle in Thailand. The sensitivity of the T.congolense IAEA/ELISA and T.evansi NIAH/ELISA was high (100%), compared to parasitological test used. About 97% of the results obtained by T.congolense IAEA/ELISA matched to the results obtained by T.evansi NIAH/ELISA. The specificity of both ELISAs with respect to cross reactions with Babesia sp., Anaplasma sp. and Theileria were not established. (author)

  6. A comparative study of an elisa test and an indirect immunofluorescence test for serological diagnosis of Babesia bovis infection

    International Nuclear Information System (INIS)

    Detection of antibodies to Babesia bovis in cattle is essential for the understanding of the epidemiology of babesiosis and this study was concerned with comparing the indirect fluorescent antibody with the ELISA. Both assays gave rise to 100% sensitivity whilst the ELISA was shown to be marginally more specific at 98%. The ease of use and low cost of the ELISA would make it the more obvious choice in conducting future serological surveys for this parasite. (author)

  7. Muestras y representatividad en vigilancia epidemiologica mediante sitios centinelas

    Directory of Open Access Journals (Sweden)

    Juan Samaja

    1996-09-01

    Full Text Available El artículo sostiene que las exigencias técnicas del muestreo para la vigilancia epidemiológica, exigen una revisión profunda de importantes conceptos de la Teoría de la Salud. En particular, es necesario hacer énfasis en las condiciones de vida, y, más específicamente, en los ambientes o contextos en que se desarrollan los procesos reproductivos de la vida social. Pero ambos campos temáticos exigen potenciar el acceso a datos más ricos que los que aportan las fuentes tradicionales. Este enfoque de la "vigilancia epidemiológica" exige una revisión de los tipos de muestras, y esto implica revisar las interpretaciones dominantes sobre los fundamentos lógicos de las inferencias a partir de muestras. Se torna necesario dejar atrás las muestras estadísticas (aún las estratificadas y promover procedimientos del tipo de los "sitios centinelas". Esta técnica, aplicada originariamente en sociedades con sistemas estadísticos deficitarios, puede desarrollarse para constituirse en un complemento substancial del monitoreo de condiciones de vida incluso en sociedades con buenos sistemas de información. El artículo propone transformar el concepto de "sitio centinela" incorporandole el requisito de la "representatividad cualitativa" mediante muestreos finalísticos sustentados en tipologías previas de las unidades espacio-poblacionales.

  8. Muestras y representatividad en vigilancia epidemiologica mediante sitios centinelas

    Directory of Open Access Journals (Sweden)

    Samaja Juan

    1996-01-01

    Full Text Available El artículo sostiene que las exigencias técnicas del muestreo para la vigilancia epidemiológica, exigen una revisión profunda de importantes conceptos de la Teoría de la Salud. En particular, es necesario hacer énfasis en las condiciones de vida, y, más específicamente, en los ambientes o contextos en que se desarrollan los procesos reproductivos de la vida social. Pero ambos campos temáticos exigen potenciar el acceso a datos más ricos que los que aportan las fuentes tradicionales. Este enfoque de la "vigilancia epidemiológica" exige una revisión de los tipos de muestras, y esto implica revisar las interpretaciones dominantes sobre los fundamentos lógicos de las inferencias a partir de muestras. Se torna necesario dejar atrás las muestras estadísticas (aún las estratificadas y promover procedimientos del tipo de los "sitios centinelas". Esta técnica, aplicada originariamente en sociedades con sistemas estadísticos deficitarios, puede desarrollarse para constituirse en un complemento substancial del monitoreo de condiciones de vida incluso en sociedades con buenos sistemas de información. El artículo propone transformar el concepto de "sitio centinela" incorporandole el requisito de la "representatividad cualitativa" mediante muestreos finalísticos sustentados en tipologías previas de las unidades espacio-poblacionales.

  9. COLECTOR SOLAR CONSTRUIDO MEDIANTE TALADRADO POR FLUENCIA TÉRMICA

    Directory of Open Access Journals (Sweden)

    Víctor Heredia R.

    2005-08-01

    Full Text Available Se diseñó y construyó un sistema de calentamiento de agua residencial, mediante dos colectores solares y un estanque. En el sistema se utilizaron tubos de cobre perforados por taladrado por fluencia térmica (TFT, unidos con soldadura de plata. El sistema funciona por termosifón y se emplea como fluido de calentamiento una mezcla de etilenglicol-agua. El fluido de calentamiento pasa a través de un serpentín de cobre a un estanque de 200 litros. Diariamente se calientan 80 litros de agua a una temperatura máxima de 45º C.A heating equipment for residential water was built, using two solar collectors and one isolated reservoir. The system uses to copper tubes perforated by thermal flow drilling (TFT, brazing with silver solder. The thermal fluid moves due to the density change of the cold and hot water. It is a mixture of ethyleneglycol-water and the heat is exchanged in the copper serpentine installed in the reservoir of 200 liters. Daily it warms 80 liters of water to 45ºC.

  10. CAPTURA DE CO2 MEDIANTE TRANSPORTADORES SÓLIDOS

    Directory of Open Access Journals (Sweden)

    Carmen Forero

    2011-01-01

    Full Text Available La evaluación de transportadores de oxígeno (TO, basados en CuO y NiO sobre Al2O3 y preparados por impregnación, se llevó a cabo en una planta piloto de dos lechos fluidizados interconectados de 500 Wte, donde se utilizaron tanto metano como gas de síntesis como gas combustible. Además, se estudió el efecto de diferentes impurezas presentes en el gas combustible como azufre o hidrocarburos ligeros en la eficacia de combustión del proceso y en el comportamiento de los TO. Los resultados obtenidos mostraron que ambos TO son adecuados para la captura de CO2 mediante transportadores sólidos de oxígeno en el proceso de combustión de metano, gas de síntesis o metano con impurezas como hidrocarburos ligeros o azufre en el gas.

  11. Desarrollo de métodos de detección de salmonella basados en la reacción en cadena de la polimerasa y su validación en muestras alimentarias

    OpenAIRE

    Martínez Ballesteros, Ilargi

    2014-01-01

    224 p. [ES] Salmonella es uno de los patógenos alimentarios más importantes a nivel mundial y causante de enfermedades gastroentéricas en humanos y animales. La presencia de Salmonella en un alimento, sea en la cantidad que sea, se considera peligroso y por lo tanto no será apto para el consumo humano. Por ello, los controles microbiológicos rutinarios en la cadena alimentaria son de vital importancia para no comprometer la salud del consumidor. Los controles se deben rea...

  12. Detección del ADN de Papilomavirus humano en lesiones precancerosas y cancerosas de cuello uterino por reacción en cadena de la polimerasa, en Bucaramanga, Santander

    Directory of Open Access Journals (Sweden)

    Castillo A.

    2001-06-01

    Full Text Available Determinar la validez de la Reacción en Cadena de la Polimerasa (PCR en el diagnóstico de la infección por el virus del papiloma humano (VPH, detectando diferentes tipos de VPH oncogénicos (16, 18, 31, 33 y 35 y no oncogénicos (6 y 11 en pacientes con lesiones precancerosas y cancerosas de cérvix y en pacientes control, estableciendo la prevalencia del virus en estas lesiones.

  13. Factores Clave de Éxito Asociados al Emprendimiento de Base Tecnológica en la Ciudad de Manizales: Un Análisis desde la Cadena de Valor del Emprendimiento

    OpenAIRE

    Rubio Jaramillo, David Andrés

    2013-01-01

    El presente trabajo busca determinar los factores clave de éxito asociados con el emprendimiento de base tecnológica en la ciudad de Manizales; se tomó como marco de referencia la cadena de valor del emprendimiento propuesta por el Ministerio de Comercio, Industria y Turismo para establecer la relevancia de cada uno de los factores clave de éxito en los diferentes eslabones. La población objeto de estudio fueron las entidades de la Red Regional de Emprendimiento de Caldas, en las cuales se in...

  14. El síndrome de fatiga crónica: del modelo de susceptibilidad a la hipótesis de la cadena de estrés en el área laboral

    OpenAIRE

    Fernando Mansilla Izquierdo

    2003-01-01

    El modelo de susceptibilidad tiene en cuenta la combinación de algunos elementos: la susceptibilidad biológica, la susceptibilidad al mobbing, la susceptibilidad al burn-out, la susceptibilidad al estrés laboral, y los factores de protección como: apoyo social, autoconcepto positivo, locus de control interno, alta autoestima y sentimiento de autoeficacia. La hipótesis de la cadena de estrés en el área laboral considera que la persistencia del estrés laboral es acumulativa. El mobbing, el sínd...

  15. Diseño de la cadena productiva sostenible del espárrago verde fresco, como alternativa de diversificación de la zona cafetera de las subregiones centro sur y bajo occidente del Departamento de Caldas

    OpenAIRE

    Salazar Bernal, Ramiro; Betancourt Escobar, Ángela María; Betancurt Quintero, Vivian Marcela; Cuellar Pavas, Oscar H.

    2004-01-01

    El diseño de la cadena productiva sostenible del espárrago verde fresco como alternativa de diversificación para la zona cafetera de las subregiones centro sur y bajo occidente del departamento de Caldas, como estudio y propuesta desarrollada por: Ramiro Salazar B. Ingeniero Industrial, Ángela María Betancourt E. Diseñadora Industrial, Oscar Cuella, Contador y Vivian Marcela Betancurt Q. Administradora de Empresas con énfasis en economía solidaria; se fundamenta y articula al plan de desarrol...

  16. Integración de los sistemas de información para la gestión de la cadena de suministro : un estudio empírico en grandes empresas

    OpenAIRE

    Gonzálvez Gallego, Nicolás

    2016-01-01

    Tesis por compendio de publicaciones Objetivos El objetivo general de esta investigación es la generación de conocimiento en torno a la relación entre las TIC, considerando de manera particular los sistemas de información, y la generación de resultados en el marco de la gestión de la cadena de suministro de grandes empresas. De manera más específica, las publicaciones de esta Tesis Doctoral persiguen dar respuesta a las siguientes preguntas de investigación: - ¿Existe una relación directa ...

  17. Del Altiplano Sur Bolivariano hasta el mercado global: Coordinación y estructuras de gobernancia de la cadena de valor de la quinua orgánica y del comercio justo

    OpenAIRE

    Pablo Laguna; Zina Cáceres; Aurélie Carimentrand

    2006-01-01

    El presente artículo tiene por objetivo describir y analizar el desarrollo de la cadena global de valor de la quinua real, asociada a la demanda de los consumidores de los mercados agroalimentarios de calidad específica. Considerando organizaciones de productores y empresas privadas locales y multinacionales, los autores abordan la evolución de las formas de coordinación y de las estructuras de gobernancia que sustentan la calidad orgánica y del comercio justo, en particular el surgimiento de...

  18. Relación entre el clima organizacional, la satisfacción laboral y la satisfacción del cliente. Caso hotel de la cadena Islazul en el oriente cubano

    OpenAIRE

    Beatriz Pupo Guisado; Gelmar García Vidal

    2014-01-01

    El presente trabajo estudia la relación entre el clima organizacional, la satisfacción laboral y la satisfacción del cliente en un hotel de la cadena cubana Islazul en la región oriental del país. La muestra estuvo conformada por 28 trabajadores de todas las áreas del mismo. El instrumento empleado fue elaborado a partir un análisis bibliométrico que evaluó los trabajos más reconocidos y aceptados por la comunidad científica de revistas indexadas en bases de datos de Redalyc. Se revisaron 325...

  19. Application of the antigen ELISA for monitoring the effectiveness of the tsetse and trypanosomosis control campaign in Zambia

    International Nuclear Information System (INIS)

    Antibody and antigen-detection ELISA were used to screen sera originating from a tsetse free area. In addition, bovine samples from tsetse infested areas were analysed using the Buffy Coat Technique (BCT) and the antigen ELISA. Few samples from a negative control population were detected positive resulting in a very good specificity for both tests. When screening a trypanosome infected population the BCT detected more acute and the Ag-ELISA more chronic infections. In conclusion, the antigen ELISA should be used in combination with the BCT, especially in geographical areas where the BCT does not detect any patent infections. (author). 2 refs, 4 figs, 4 tabs

  20. Detection of infectious laryngotracheitis virus antibodies by glycoprotein-specific ELISAs in chickens vaccinated with viral vector vaccines.

    Science.gov (United States)

    Godoy, Alecia; Icard, Alan; Martinez, Mellisa; Mashchenko, Anna; García, Maricarmen; El-Attrachea, John

    2013-06-01

    Two glycoproteins of infectious laryngotracheitis virus (ILTV), gI and gB, were expressed in baculovirus and purified for the development of ILTV recombinant protein-based ELISAs. The ability of gB and gI ELISAs to detect ILTV antibodies in chickens vaccinated with viral vector vaccines carrying the ILTV gB gene, Vectormune FP-LT (the commercial fowlpox vector laryngotracheitis vaccine) and Vectormune HVT-LT (commercial turkey herpesvirus vector laryngotracheitis vaccine), was evaluated using serum samples from experimentally vaccinated and challenge chickens. The detection of gB antibodies in the absence of gI antibodies in serum from chickens vaccinated with FP-LT indicated that the gB ELISA was specific for the detection of antibodies elicited by vaccination with this viral vector vaccine. The gB ELISA was more sensitive than the commercial ILTV ELISA to detect seroconversion after vaccination with the FP-LT vaccine. Both gI and gB antibodies were detected in the serum samples collected from chickens at different times postchallenge, indicating that the combination of these ELISAs was suitable to screen serum samples from chickens vaccinated with either recombinant viral vector FP-LT or HVT-LT vaccines. The agreement between the gI ELISA and the commercial ELISA to detect antibodies in serum samples collected after challenge was robust. However, further validation of these ELISAs needs to be performed with field samples. PMID:23901757

  1. Avaliação do desempenho do método do ensaio por enzimas imuno-adsorvidas (ELISA em leite em pó reconstituído contaminado experimentalmente com aflatoxina M1 Evaluation of competitive enzyme-linked immunosorbent assay (ELISA in milk powder contaminated with known concentrations of aflatoxin M1

    Directory of Open Access Journals (Sweden)

    Carlos Augusto Fernandes de Oliveira

    1996-12-01

    Full Text Available O desempenho do ensaio por enzimas imuno-adsorvidas (ELISA, mediante o emprego de conjuntos de reativos produzidos em escala comercial, para determinação de aflatoxina, foi avaliado em condições experimentais, através de análises repetidas, em amostras de leite em pó reconstituído contaminadas com concentrações conhecidas da fração M1 da toxina. Para os níveis de 0,10; 0,20; 0,50; e, 1,00 ng/ml, os percentuais de recuperação foram: 83,0%; 87,5%; 103,0%; e, 111,8%, respectivamente. O desvio-padrão relativo, para as referidas concentrações, foi, respectivamente, 65,5%; 31,8%; 10,9% e 13,6% (n=10, para cada nível de contaminação. Os resultados obtidos demonstram que o método é apropriado para pesquisas e levantamentos sobre a ocorrência de aflatoxina M1 em leite, sobretudo nas faixas de concentração entre 0,20 - 1,00 ng/ml.The evaluation of commercially available test systems of competitive enzyme-linked immunosorbent assay (ELISA, for Aflatoxin M1 (AFM1 was performed in experimental conditions, through repeated analysis, in samples of milk powder contamined with known concentrations of the toxin. Recoveries of AFM1 added to milk at levels of 0.10, 0.20, 0.50 and 1.00 ng/ml were 83.0%, 87.5%, 103.0% and 111.8% respectively. Relative standard deviations for the above mentioned concentrations were 65.5%, 31.8%, 10.9% and 13.6%, respectively (n =10, per spiking level. According to these results the ELISA is appropriate for AFM1 research and/or surveying, mainly for concentrations between 0.20 - 1.00 ng/ml.

  2. EVALUACIÓN DE LA CADENA DE FRÍO EN CINCO FÁBRICAS DE CECINAS Y SU IMPLICANCIA EN EL RECUENTO MICROBIOLÓGICO EVALUATION OF THE COLD CHAIN IN FIVE PROCESSED MEAT FACTORIES IN CHILE AND THE IMPLICATIONS FOR THE MICROBIOLOGICAL COUNT

    OpenAIRE

    Waldo Armstrong G; Luis Quintana D; Verónica Chesta S

    2012-01-01

    El Reglamento Sanitario de los Alimentos Chileno regula la fabricación, distribución y almacenamiento de éstos. Debido a la falta de datos publicados sobre la aplicación del reglamento, se evaluó la continuidad de la cadena de frío en 5 fábricas de cecinas, utilizando paté y queso de cabeza como productos controles. Los resultados mostraron deficiencias en la mantención de la cadena de frío, principalmente en la distribución y venta minorista de productos. Además, se detectaron diferencias es...

  3. Planificación táctica de las operaciones en cadenas de suministro de respuesta rápida (responsive) con estructura alternativa de procesos: modelado matemático, implementación y experimentación

    OpenAIRE

    CALDERÓN LAMA, JOSÉ LUIS

    2011-01-01

    Se define la Cadena de Suministro de Respuesta Rápida (Responsive Supply Chain) como aquella apropiada para productos innovadores con procesos estables. Esta Cadena de Suministro (CS) tiene que enfrentar una incertidumbre alta en la previsión de la demanda de sus múltiples productos (demanda que es además estacional y volátil). Interesa reducir los costes, las roturas de stock y el exceso de productos que deberán rematarse al final de cada temporada de ventas. El objetivo de esta tesis e...

  4. Control mediante SCADA de un panel con elementos neumáticos

    OpenAIRE

    PERIS MARTINEZ, SERGI

    2015-01-01

    [ES] Se realiza y explica un control mediante un sistema SCADA sobre unos elementos neumáticos de agarre, posicionamiento y desplazamiento. Éstos estarán accionados con electroválvulas, controladas mediante un autómata programable. También se muestra lo que podría ser un ejemplo de un proceso industrial utilizando los elementos anteriores para hacer el control de dicho procedimiento.

  5. Detection of Brucella antigen in the aborted ovine fetal stomach contents using a modified ELISA test

    Directory of Open Access Journals (Sweden)

    Th. M. Al-Nima

    2010-01-01

    Full Text Available This study was conducted on two flocks of sheep suffering from abortion in Mosul city, Iraq. The clinical findings in ewes were abortion during the 3-4 months of gestation period in the both flocks. The total percentage of abortion was 11.7 %, whereas stillbirth percentage was 4 %. Brucella spp. was isolated from four (33.3 % of the 12 samples (stomach contents of the aborted fetuses. All culture – positive samples had also positive with direct smears. By a modified enzyme-linked immunosorbent assay (ELISA, Brucella antigens were detected in the fetal stomach contents of 5 samples. The sensitivity and specificity of the modified ELISA were 100 % and 87.5 % respectively. The test had a good negative predictive value but only a moderate positive predictive value. Therefore, the test would be useful for confirming the existence of suspect disease. Comparison of modified ELISA with bacterial isolation demonstrated a close agreement (Kappa value = 0.92. Of the 12 serum samples from aborted ewes, eight samples were positive with Rose Bengle test (66.7 %, more than 10 samples (83.3 % were detected by indirect ELISA test.

  6. Agricultural production - Phase 2. Indonesia. Implementation of ELISA for brucellosis at DGLS laboratories in indonesia

    International Nuclear Information System (INIS)

    This expert mission was performed to assess the current status of activities relating to brucellosis testing at the Regional Disease Investigation Centres of the Directorate General of Livestock Services in Indonesia, and to assist in the establishment and validation of the ELISA technique for detecting Brucella antibodies

  7. Agricultural production - Phase 2. Indonesia. National training course on ELISA for seradiagnosis of animal diseases (5)

    International Nuclear Information System (INIS)

    This report describes the content of a three-week national training course for 16 participants from regional Disease Investigation Centres and other agencies in Indonesia. The subject of the course was the use of ELISA for the diagnosis of animal diseases in Indonesia, with particular emphasis placed on bovine brucellosis

  8. Transfer of Trypanosoma ELISA technology to Onderstepoort Veterinary Institute, South Africa

    International Nuclear Information System (INIS)

    Following a brief historical overview of trypanosomosis in South Africa, the present day role is explained of the Onderstepoort Veterinary Institute (OVI) in assisting disease diagnosis and surveillance. Production and distribution of ELISA kits will be initiated at OVI, initially for brucellosis and at a later stage for trypanosomosis. (author)

  9. Competitne elisa for the detection of gluten hydrolysate and applications thereof.

    OpenAIRE

    Méndez Cormán, Enrique; García Ortiz, Enrique; Ferre López, Sergio

    2006-01-01

    The invention relates to a competitive ELISA kit, which is used to identify gluten hydrolysate in food and raw materials, and to the use of said kit. The inventive kit is advantageous over other kits in that it can be used to identify gluten hydrolysate.

  10. Use of the ELISA in studies on tropical theileriosis in Morocco

    International Nuclear Information System (INIS)

    An enzyme-linked immunosorbent assay (ELISA) was developed for use in sero-epidemiological studies of theileriosis caused by Theileria annulata in Morocco. The antigen used was prepared from sonicated whole purified piroplasmas. The ELISA was used to study the serological responses in calves which where experimentally immunized with a schizont-infected attenuated cell line and challenged a month later with a lethal dose of sporozoites. The test was also used to study post vaccinal antibodies in pregnant cows and in animals immunized with different doses of the attenuated cell lines at different levels of passage. Finally, the test was used to screen sear collected from cattle in an area where infection with T. annulata was endemic before and after the theileriosis season. Sera from animals in the above studies were also analyzed using an indirect fluorescent antibody test (IFAT) with T. annulata piroplasmas in order to compare the relative sensitivities of the two tests. It was found that the piroplasma stage of T. annulata was a more appropriate stage from which to prepare antigen for use in ELISA then the sporozoite or schizont stages. A marked heterogeneity in antibody response was observed in animals vaccinated using an attenuated cell line vaccine, however, little difference in this response was seen between exotic and local animals which had not been previously exposed to infection with T. annulata. A concordance level of 91.5% was found between diagnosis by blood smears and ELISA. (author). 9 refs, 4 figs, 2 tabs

  11. A Direct, Competitive Enzyme-Linked Immunosorbent Assay (ELISA) as a Quantitative Technique for Small Molecules

    Science.gov (United States)

    Powers, Jennifer L.; Rippe, Karen Duda; Imarhia, Kelly; Swift, Aileen; Scholten, Melanie; Islam, Naina

    2012-01-01

    ELISA (enzyme-linked immunosorbent assay) is a widely used technique with applications in disease diagnosis, detection of contaminated foods, and screening for drugs of abuse or environmental contaminants. However, published protocols with a focus on quantitative detection of small molecules designed for teaching laboratories are limited. A…

  12. A recombinant nucleocapsid-based indirect ELISA for serodiagnosis of Rift Valley fever in African wildlife

    International Nuclear Information System (INIS)

    An indirect ELISA (I-ELISA) based on the recombinant nucleocapsid protein (rNp) of Rift Valley fever virus (RVFV) was evaluated for the detection of specific serum IgG antibody in African wildlife. Data sets derived from field-collected sera (n = 918) in Africa (antelopes = 570, black rhinoceros = 43, common zebra = 24, elephant = 73, giraffe = 81, grevy zebra = 78, warthog = 49) were categorised according to the results of a virus neutralisation test (VNT). At cut-offs optimised by the two-graph receiver operating characteristics analysis, the diagnostic sensitivity of the I-ELISA was 100% and diagnostic specificity ranged from 99.8% to 100% while estimates for the Youden's index (J) and efficiency (Ef) ranged from 0.99 to 1 and from 99.7% to 100%, respectively. The rNp-based I-ELISA is highly accurate, safe, and offers a single assay format for rapid detection of IgG antibody to RVFV in sera of different wildlife species. (author)

  13. Evaluation of an indirect ELISA for the diagnosis of Babesia bovis in Uruguay

    International Nuclear Information System (INIS)

    In initially establishing the FAO/IAEA indirect ELISA for the detection of antibodies to Babesia bovis, the optical density (OD) values of sera from known positive or negative local cattle were compared to the OD values obtained from the negative and positive reference sera provided with the ELISA kit. The percentage of false positive and negative sera were 2.53% and 2.97% respectively. The cut-off values for the negative reference serum in the kit were compared with those of a local negative population. These values were found to be similar. The specificity of the test was evaluated by testing 30 sera from animals experimentally infected with Anaplasma marginale and 30 sera from animals infected with Babesia bigemina. These were no cross-reaction either between A. marginale and B. bovis or between B. bigemina and B. bovis. A serological survey using this ELISA kit was carried out on animals from an enzootic area and an area free from the vecot Boophilus microplus. 53 out of 282 animals (18.8%) in the enzootic area were positive whilst all the animals (113) from the free area were negative. This study would indicate that the FAO/IAEA ELISA kit has a sensitivity of around 98% and specificity of 97%. (author). 8 refs, 2 figs, 2 tabs

  14. Validation of an indirect ELISA for the diagnosis of Babesia bovis in cattle in Yucatan, Mexico

    International Nuclear Information System (INIS)

    The ELISA kit provided by the FAO/IAEA for the diagnosis of Babesia bovis was validated. In order to determine the appropriate ELISA cut-off point that would serve as the threshold between positive and negative samples, 119 serum samples from a Mexican Babesia-free zone were analyzed. The optimal cut-off point chosen was at 12% of the reactivity of the high positive control serum sample (PP) which resulted in a specificity of 97%. One hundred and ninety-six cattle from Wisconsin, USA, were introduced into Yucatan, Mexico, of which 181 were vaccinated with an attenuated live Babesia bovis vaccine; 15 animals remained as unvaccinated controls. Before and after vaccination all animals were bled and tested by enzyme linked immunosorbent assay (ELISA) and indirect fluorescence antibody test (IFAT). Both tests showed a high degree of correlation in their results. To evaluate an immune response to vaccination the optimal cut-off point chosen was 12% PP resulting in a sensitivity 99% and a specificity 95%. We concluded that the ELISA test has proved to be useful in Yucatan, Mexico for serological surveys and monitoring the efficiency o vaccination programmes. (author)

  15. ELISA and modified toxin-binding inhibition test for quality control of the clostridial vaccine processes

    Directory of Open Access Journals (Sweden)

    E.M. Sobrinho

    2014-06-01

    Full Text Available This study aimed to assess and standardize the ELISA and modified ToBI test in vitro methods in order to verify the potency of epsilon toxicoid in comparison with the in vivo TCP method. The following epsilon toxoids were used: NIBSC standard from batches 375/07, 532/08, 551/08, 373/07 and 378/07. These were evaluated using a TCP test, ELISA and ToBI tests. The results indicate that the correlation ratio between the dilutions of standard NIBSC toxicoid and absorbance values of 89.44% obtained with the ELISA method support the use of the curve to evaluate epsilon toxoids. However, it was observed that the absorbance values were similar for all toxoids, thus presenting no significant difference between higher and lower concentration toxoids. For the ToBI test, the correlation ratio of 96.76, obtained in the curve pattern, demonstrates the effectiveness of the curve to be used in the epsilon toxoid evaluation. The correlation ratio between the titration degrees of toxoids obtained through TCP and ToBI tests was higher than 90%. It is concluded that the type of ELISA test used does present discriminative power for toxoids with different concentrations, which does not support the use of this technique for such a purpose. The ToBI test can be used as a screening method for it is sensitive and effective to detect epsilon toxicoid produced by C. perfringens type D.

  16. Evaluation of an ELISA for the routine diagnosis of Dictyocaulus viviparus infections in cattle

    NARCIS (Netherlands)

    Cornelissen, J.B.W.J.; Borgsteede, F.H.M.; Milligen, van F.J.

    1997-01-01

    An enzyme-linked immunosorbent assay (ELISA) that detects antibodies against Dictyocaulus viviparus in experimentally and naturally infected cattle was evaluated for its sensitivity, specificity, the moment of seroconversion and persistence of the anti-D. viviparus response and precision. The first

  17. USE OF ENZYME-LINKED IMMUNOSORBENT ASSAYS (ELISA) FOR THE DETERMINATION OF TRITON X NONIONIC DETERGENTS

    Science.gov (United States)

    An enzyme-linked immunosorbent assay (ELISA) for 4-t-octylphenyl ethoxylates such as Triton X-100 was developed. Both the 4-t-octylphenyl and the ethoxylate moiety were required for antibody recognition since members of the Triton N series showed low cross-reactivity, and polyeth...

  18. DETERMINATION OF 3,5,6-TRICHLORO-2-PYRIDINOL (TCP) BY ELISA

    Science.gov (United States)

    A sensitive, competitive enzyme-linked immunosorbent assay (ELISA) for 3,5,6-trichloro-2pyridinol (TCP) has been developed to quantitate parts per billion (ppb) amounts of the analyte in urine. TCP is a major metabolite and environmental degradation product of the insecticide c...

  19. Combined use of random access and ELISA analyzers in the microbiological serology laboratory

    OpenAIRE

    Alessandra Moroni; Antonella Marangoni; Marina Biagi; Silvia Capitani; Enzo Della Bella; Sanzio Ruscello; Franca Savioli; Roberto Cevenini

    2008-01-01

    In the last years the trend of centralizing small laboratories in large reference centers led to a careful evaluation of the diagnostic profiles. In the serology laboratory of Microbiology Unit, St. Orsola-Malpighi Hospital, Bologna, Italy the choice has been to combine random access analyzers (ARCHITECT Abbott) and ELISA analyzers (BEPIII Dade Behring).

  20. Combined use of random access and ELISA analyzers in the microbiological serology laboratory

    Directory of Open Access Journals (Sweden)

    Alessandra Moroni

    2008-09-01

    Full Text Available In the last years the trend of centralizing small laboratories in large reference centers led to a careful evaluation of the diagnostic profiles. In the serology laboratory of Microbiology Unit, St. Orsola-Malpighi Hospital, Bologna, Italy the choice has been to combine random access analyzers (ARCHITECT Abbott and ELISA analyzers (BEPIII Dade Behring.

  1. Evaluation of commercial ELISA kits for detection of antibodies against bovine atypical pestivirus

    DEFF Research Database (Denmark)

    Larska, Magdalena; Polak, Mirosław P.; Uttenthal, Åse;

    A group of emerging bovine pestiviruses becomes a possible threat to Bovine Viral diarrhea virus (BVDV) control and eradication programs in the countries of their origin and in the new continents due to the lack of validated detection methods. The use of ELISA kits may be acheaper, time saving and...

  2. A serological survey using ELISA for Babesia bovis infection of cattle in Turkey

    International Nuclear Information System (INIS)

    A total of 1428 serum samples collected from cattle in six different regions of Turkey during 1986 to 1989 were screened for antibodies against Babesia bovis. A recombinant DNA B. bovis - derived antigen was used in an indirect ELISA. The results indicated the presence of B. bovis antibodies in 51.2% of the cattle. (author). 6 refs, 1 tab

  3. EVALUATION OF AN O-ANTIGEN ELISA FOR SCREENING CATTLE HERDS FOR SALMONELLA-TYPHIMURIUM

    DEFF Research Database (Denmark)

    Hoorfar, Jeffrey; Bitsch, V.

    1995-01-01

    several years, and 12 herds from a salmonella enzootic area which had had clinical outbreaks of S typhimurium were used to define a herd ELISA cut-off value. When herds with at least 5 per cent of the serum samples having an optical density of >0.5 were considered ELISA-positive, all 10 herds from the...... antibody to the 0:9 antigen of the serogroup D salmonellas, indicating the possibility of rapid serogroup-specific screening of herds by means of these two tests. Ten other randomly selected herds with clinical outbreaks of S dublin were all, to a large extent, positive in the 0:1,4,5,12-ELISA, whereas a S......A total of 2585 serum samples from 62 dairy herds located in four different regions of Denmark were tested in an O-antigen (0:1,4,5,12)-based ELISA for the detection of antibodies against Salmonella typhimurium. Ten closed herds from an island with no reported occurrence of salmonellosis for...

  4. ELISA and other methods in monitoring vaccination response to haemorrhagic septicemia

    International Nuclear Information System (INIS)

    Two groups each of 15 buffaloes were vaccinated subcutaneously or intramuscularly with a Pasteurella multocida vaccine consisting of crude capsular antigen. No significant differences in immune response were observed between the two groups using the indirect haemagglutination assay. Sera from the vaccinated buffaloes were subjected to the passive mouse protection test and there was an overall average survival rate of 87.5% when the mice were challenged with 100LD50 of the homologous vaccine strain. In a second trial, 67 buffaloes were grouped into 7 groups. Six of the groups were vaccinated with whole cell or crude capsular antigen respectively combined with water, alum or an oil adjuvant respectively. The seventh group (group VII) was not vaccinated and served as the control. Sera collected from these groups were tested by an enzyme-linked immunosorbent assay (ELISA) and the results obtained analyzed by Duncan's multiple range test. The antibody responses of vaccinated groups were statistically different to the control group. Serum from these buffaloes were grouped according to their ELISA optical density values (x dilution factor) and inoculated into mice. One hundred percent protection was provided by sera with an ELISA titre between 71-90 collected 3 months after vaccination while 11 months after vaccination 100% protection was provided but only with sera having ELISA titres greater than 90. (author). 9 refs, 3 figs, 2 tabs

  5. Development of hen antihepatitis B antigen IgY-based conjugate for ELISA assay

    Directory of Open Access Journals (Sweden)

    Najat Muayed Nafea

    2015-01-01

    Conclusions: This study showed that laying hens can be used as an alternative source for production of polyclonal antibodies against HBsAg and anti-HBs IgY could be labeled with HRP enzyme and could subsequently be used successfully as secondary antibody in ELISA for detection of HBsAg in the patients sera.

  6. International ring trial to detect anti-Trichinella IgG by ELISA on pig sera.

    Science.gov (United States)

    In the present study, the sensitivity and specificity of the ELISA assay determined by the CRLP validation was 100% and 98.29%, respectively. The assay was reproducible, moreover, based on the receiver-operator characteristic (ROC) curve, the sensitivity and specificity of the assay reached 97.5% an...

  7. Evaluation of an indirect elisa for the diagnosis of bovine brucellosis in Patagonia, Argentina

    International Nuclear Information System (INIS)

    Control and eradication of bovine brucellosis is usually based on the serological detection of antibodies. In Argentina, the Rose Bengal test (RB) and the Buffered Plate antigen test (BPA) are the two screening test officially recognized, while the 2-mercaptoethanol test (2ME) and the Tube Agglutination test (SAT) are the confirmatory assays currently in use. In order to improve the serological diagnosis of bovine brucellosis in Patagonia, Argentina, an indirect ELISA kit produced by the Joint FAO/IAEA Division was evaluated. Sera from negative non-vaccinated, negative but vaccinated and positive animals were tested by all the above techniques. The specificity of the I-ELISA (99.6% and 99.7%) was similar to that of the BPA, RB, 2ME and Complement Fixation test (CF) when used to test sera from non-vaccinated, negative and vaccinated, negative animals, respectively. The sensitivity of the I-ELISA (98%) was higher than the BPA test (96%) and the CF test (95,2%). The I-ELISA kit evaluated in this study was thought to be a valuable tool for the diagnosis of bovine brucellosis in Patagonia region where little epidemiological information is available about this disease and where large numbers of sera should be tested to obtain such information. (author)

  8. RT-PCR-ELISA as a tool for diagnosis of low-pathogenicity avian influenza

    DEFF Research Database (Denmark)

    Dybkaer, Karen; Munch, Mette; Handberg, Kurt Jensen; Jørgensen, Poul H

    A one-tube reverse transcriptase/polymerase chain reaction coupled with an enzyme-linked immunosorbent assay (RT-PCR-ELISA) was developed for the rapid detection of avian influenza virus (AIV) in clinical specimens. A total of 419 swab pools were analyzed from chickens experimentally infected with...

  9. Diagnosis of bovine brucellosis in Mosul city by indirect ELISA and conventional serological tests

    Directory of Open Access Journals (Sweden)

    O. KH. AL-Hankawe

    2010-01-01

    Full Text Available The study was conducted on 126 cattle (94 females and 32 males of different ages (1->5 years randomly selected from July 2007 to August 2008 in Mosul. Indirect ELISA test and other traditional tests (rose Bengal test, tube agglutination test and 2- mercapto-ethanol test were used to determine the incidence of bovine brucellosis. The highest incidence of disease was recorded by Indirect ELISA, 23.01%, whereas it was 18.25%, 11.90% and 4.76% by rose Bengal, tube agglutination and 2- Mercapto-ethanol tests, respectively. The highest incidence was in females in all serological tests and the highest incidence was in females at the age between 1-3 years whereas in males more than 3 years of age it was 23.07%. The results of tube agglutination test revealed the titer 1/40 occurred mostly compared with other titers. Six chronic cases were determined by 2-mercapto-ethanol test. The degree of agreement of negative samples with rose Bengal test and indirect ELISA, tube agglutination, and 2- mercapto-ethanol tests was 94.17%, 100% and 100%, respectively, and by indirect ELISA with rose Bengal, tube agglutination and 2-mercapto-ethanol tests was 79.31%, 51.72% and 20.68%, respectively.

  10. Rapid Detection of Food Allergens by Microfluidics ELISA-Based Optical Sensor

    Directory of Open Access Journals (Sweden)

    Xuan Weng

    2016-06-01

    Full Text Available The risks associated with the presence of hidden allergens in food have increased the need for rapid, sensitive, and reliable methods for tracing food allergens in commodities. Conventional enzyme immunosorbent assay (ELISA has usually been performed in a centralized lab, requiring considerable time and sample/reagent consumption and expensive detection instruments. In this study, a microfluidic ELISA platform combined with a custom-designed optical sensor was developed for the quantitative analysis of the proteins wheat gluten and Ara h 1. The developed microfluidic ELISA biosensor reduced the total assay time from hours (up to 3.5 h to 15–20 min and decreased sample/reagent consumption to 5–10 μL, compared to a few hundred microliters in commercial ELISA kits, with superior sensitivity. The quantitative capability of the presented biosensor is a distinctive advantage over the commercially available rapid methods such as lateral flow devices (LFD and dipstick tests. The developed microfluidic biosensor demonstrates the potential for sensitive and less-expensive on-site determination for rapidly detecting food allergens in a complex sample system.

  11. Evaluation of an inhouse rapid ELISA test for detection of giardia in domestic sheep (Ovis aries).

    Science.gov (United States)

    Wilson, Jolaine M; Hankenson, F Claire

    2010-11-01

    Sheep (Ovis aries) are increasingly used at our institution as models of human disease. Within the research environment, routine husbandry and handling of sheep has potential for transmission of zoonotic agents, including Giardia. The prevalence of Giardia in sheep may approach 68%. Classic diagnostic testing involves microscopic examination for fecal cysts or trophozoites; however, limitations of microscopy include time, labor, and potential false-negative results due to intermittent shedding. We wished to determine whether a commercial rapid ELISA used for Giardia detection in dogs and cats could be used in sheep. Fecal samples collected from sheep (n = 93) were tested with a combination of 6 methods: reference laboratory fecal flotation, reference laboratory ELISA, inhouse fecal flotation, and commercially available tests (enzyme immunoassay, direct fluorescence antibody assay, and rapid ELISA). Prevalence of Giardia infection in facility sheep was 11.8% (11 of 93 animals). Of the 11 samples considered positive, 3 were confirmed by multiple testing methods, and 5 were positive by microscopy alone. Inhouse fecal flotation for 8 samples was positive on only 1 of 2 consecutive testing days. The rapid ELISA test exhibited 0% sensitivity for sheep giardiasis. Overall, the examined methods had low sensitivities and low positive predictive values. Despite limitations, microscopic analysis of repeat fecal samples remained the most accurate diagnostic method for ovine giardiasis among the methods tested. PMID:21205445

  12. ELISA, a Small Experiment For a Large Sub-millimeter Survey

    Science.gov (United States)

    Bernard, J. P.; Ristorcelli, I.; Armengaud, M.; Crussaire, J. P.; Giard, M.; Lagache, G.; Lamarre, J.-M.; Leriche, B.; Longval, Y.; Meny, C.; Stepnik, B.; Torre, J.-P.

    This paper presents the scientific objectives and technical aspects of the balloon-borne Experiment for Large Infrared Survey Astronomy (ELISA). The emphasis is put upon the synergies existing between the ELISA project and future space missions, both with respect to scientific and technical aspects. ELISA is a small balloon project for an experiment dedicated to measure the Far-Infrared to Sub-millimeter continuum emission of Galactic dust over a large fraction of the sky, with unprecedented sensitivity and angular resolution. The primary mirror of the telescope, similar to the one used for the Top-Hat mission, will have a diameter of 1 m, ensuring an angular resolution of about 3.5'. Bolometer arrays will be used in four photometric bands centered at 170, 240, 400, and 650 {μ m} and providing a 22' × 45' instantaneous field of view per channel. A liquid He cryostat will host the cold optics, including the secondary mirror of the telescope, as well as the detectors, which will be cooled to 0.3 K using an ^3He close-cycle fridge. Mapping of the sky will be accomplished by rotating the gondola over a large azimuth range (up to 60{^circ} amplitude). The pointing of the experiment will be maintained to a constant elevation during the azimuth scans through a feed back loop using the signal from a large format, fast stellar sensor, operating day and night. The scientific goal of the experiment is to map the diffuse Sub-millimeter emission along a large fraction of the Milky Way. The astronomical data obtained will be used to derive the emission properties of the dust grains in the Interstellar Medium (ISM), such as their temperature and emissivity. It will also allow to systematically measure the polarization of the dust emission. It should also lead to the detection of a few thousand point sources such as newly formed stars and distant galaxies. In addition to these goals, the ELISA project will serve as a test bed for the detector technology that will be used for the

  13. ELISA versus PCR for diagnosis of chronic Chagas disease: systematic review and meta-analysis

    Directory of Open Access Journals (Sweden)

    Hasslocher-Moreno Alejandro M

    2010-11-01

    Full Text Available Abstract Background Most current guidelines recommend two serological tests to diagnose chronic Chagas disease. When serological tests are persistently inconclusive, some guidelines recommend molecular tests. The aim of this investigation was to review chronic Chagas disease diagnosis literature and to summarize results of ELISA and PCR performance. Methods A systematic review was conducted searching remote databases (MEDLINE, LILACS, EMBASE, SCOPUS and ISIWeb and full texts bibliography for relevant abstracts. In addition, manufacturers of commercial tests were contacted. Original investigations were eligible if they estimated sensitivity and specificity, or reliability -or if their calculation was possible - of ELISA or PCR tests, for chronic Chagas disease. Results Heterogeneity was high within each test (ELISA and PCR and threshold effect was detected only in a particular subgroup. Reference standard blinding partially explained heterogeneity in ELISA studies, and pooled sensitivity and specificity were 97.7% [96.7%-98.5%] and 96.3% [94.6%-97.6%] respectively. Commercial ELISA with recombinant antigens studied in phase three investigations partially explained heterogeneity, and pooled sensitivity and specificity were 99.3% [97.9%-99.9%] and 97.5% [88.5%-99.5%] respectively. ELISA's reliability was seldom studied but was considered acceptable. PCR heterogeneity was not explained, but a threshold effect was detected in three groups created by using guanidine and boiling the sample before DNA extraction. PCR sensitivity is likely to be between 50% and 90%, while its specificity is close to 100%. PCR reliability was never studied. Conclusions Both conventional and recombinant based ELISA give useful information, however there are commercial tests without technical reports and therefore were not included in this review. Physicians need to have access to technical reports to understand if these serological tests are similar to those included in

  14. Utilización de los test de Fluorescencia Polarizada (FP y Elisa de Competencia (C-Elisa en el diagnóstico de brucelosis de camélidos The use of polarized fluorescence assay (PF and competitive ELISA test (C-Elisa for the diagnosis of Brucellosis in South American camelids

    Directory of Open Access Journals (Sweden)

    X Rojas

    2004-01-01

    Full Text Available En Chile, como en muchos países de Latinoamérica, la brucelosis es una zoonosis presente, representando no sólo un factor de riesgo para las explotaciones pecuarias sino también para quienes laboran y hacen usufructo de ella, especialmente por el consumo de leche cruda y sus derivados. Como la Brucella afecta a diferentes especies de animales, el objetivo de este trabajo fue utilizar las técnicas de unión primaria, como son Fluorescencia Polarizada (FP y Elisa de Competencia (C-Elisa, para la detección de anticuerpos anti-Brucella en suero de camélidos y, comparar sus respuestas con las pruebas de Rosa de Bengala (RB, Aglutinación Lenta en Tubo (SAT y Fijación de Complemento (FC en atención a que estas especies representan un potencial pecuario de cierta trascendencia. Para ello se analizaron 336 sueros, de los cuales 315 pertenecían a llamas y 21 a alpacas, provenientes de un predio de la Novena Región clínica y serológicamente libre de Brucella. Los resultados obtenidos señalan que el umbral de positividad para FP, calculado según la mediana del último percentil, fue de 98.7 milipolarizaciones (mP y, el corte para C-Elisa con el mismo método fue de 32% de Inhibición (% I. Todas las muestras analizadas dieron resultados negativos a RB y SAT. Los resultados con FC no pudieron ser considerados, por cuanto un alto porcentaje de las muestras fueron anticomplementarias. Con las técnicas de C-Elisa y FP, dos muestras reaccionaron positivamente en cada uno de los test con valores en el nivel de corte o levemente superiores. Se discute la significación de ellas.Brucellosis is a zoonotic disease representing a risk contact with, or consuming, their raw milk and raw dairy several animal species, including camelids, the aim of this Polarization assay (PF and Competitive ELISA (C-ELISA, along with conventional serological tests such as the Rose Complement Fixation test (CF. A total of 336 serum samples herd of the IXth Region (Chile

  15. Climático: evaluación mediante modelos

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    P. Ruiz-Benito

    2013-01-01

    Full Text Available Los bosques son ecosistemas fundamentales en la generación de servicios ecosistémicos y, por tanto, para el bienestar humano. El cambio global (incluyendo cambio climático y cambios en el uso del suelo puede, sin embargo, alterar la dinámica y el funcionamiento de los ecosistemas, afectando al futuro suministro de servicios ecosistémicos. La vulnerabilidad frente al cambio global depende de la exposición (magnitud del cambio, la sensibilidad (susceptibilidad al cambio, y la capacidad de adaptación (habilidad para ajustarse al cambio de las especies. En el presente trabajo presentamos diversas aproximaciones de modelización que permiten analizar los diferentes componentes de la vulnerabilidad, e incluimos ejemplos desarrollados para bosques de la península Ibérica. A pesar de estos avances, la evidencia empírica y teórica para integrar los impactos potenciales (i.e. incluyendo la exposición y la sensibilidad y la capacidad de adaptación de las especies, es escasa. Por ello, para una adecuada evaluación sería necesario me-jorar el conocimiento existente sobre la sensibilidad y capacidad de adaptación de las especies y su respuesta frente a cambios ambientales extremos (por ejemplo, mediante redes de seguimiento a largo plazo, integrando adecuadamente la información obtenida en modelos que incluyan procesos basados en diferentes niveles de organización biológica, desde procesos fisiológicos a modelos agregados de distribución de especies.

  16. Assessment of an indirect ELISA for the diagnosis of bovine tuberculosis

    International Nuclear Information System (INIS)

    The sensitivity and specificity of an ELISA for the detection of bovine IgG anti-Mycobacterium bovis (M. bovis) antibodies were 73.6% and 94.1% respectively, as determined in 53 bacteriologically confirmed tuberculous cattle and 101 healthy cattle from a tuberculosis-free area. In addition, the results of ELISA and tuberculin tests in 149 cattle were compared with those of subsequent necropsy studies. Both tests failed to detect 2 animals with tuberculous lesions and positive M. bovine culture. Three of 12 cattle, in which M. bovine was isolated but no lesions were found, reacted to tuberculin as did 2 of 7 cattle that had atypical mycobacterial infection. In none of these animals was it possible to detect antibody titres against M. bovis. In 128 cattle that had no lesions nor the presence of mycobacteria, 6 were tuberculin reactors and 7 others had antibody titres against M. bovis. Negative results were obtained by ELISA in 21 of 22 paratuberculous cattle. Antibody titres were not detected in 88.9% to 96.4% of 697 cattle in three tests from two tuberculin negative herds of an endemic area. In a herd with proven M. bovis infection, the distribution of seropositive animals in tuberculin and non-tuberculin reactors was similar. Antibody responses to cutaneous tuberculin stimuli were observed in 4 cattle experimentally infected with M. bovis, but only in 2 of 10 healthy controls after repeated PPD stimuli. Nine controls which had either received a single tuberculin dose or no inoculation showed no increase in antibody levels. The low sensitivity of this ELISA limits its usefulness as a diagnostic tool for identifying individual tuberculous animals in eradication campaigns. However, it could be helpful in epidemiological surveillance if further field testing indicates that infected herds can be identified by ELISA. (author). 21 refs, 2 figs, 2 tabs

  17. Validación operacional de un ELISA comercial para Brucella ovis, Chile Operational validation of a commercial ELISA for Brucella ovis, Chile

    OpenAIRE

    J F Alvarez; R Veneros; González, O.

    2007-01-01

    La sensibilidad y especificidad diagnóstica se estiman para una población de referencia, estas difieren de la interpretación operacional o de terreno. El objetivo del presente trabajo es validar operacionalmente un ELISA (Enzyme-Linked Immunosorbent Assay) comercial para Brucella ovis en la población ovina de Magallanes. Se emplearon 82 sueros de carneros con aislamiento de B. ovis en semen y 421 sueros de animales con cultivo microbiológico de semen negativo e inmunodifusión en agar gel con ...

  18. Diagnóstico ecológico y económico de la cadena de suministros para el reciclaje de plásticos en el contexto empresarial cubano

    Directory of Open Access Journals (Sweden)

    Michael Feitó Cespón

    2015-01-01

    Full Text Available La cadena de suministros para el reciclaje de plásticos en Cuba ha estado presentando un bajo desempe ̃ no durante varios a ̃ nos, por lo tanto el objetivo de este trabajo es realizar un diagnóstico estratégico de su gestión que integre las dimensiones económicas y medioambientales. Para conseguir este propósito se utilizan los procesos de pensamiento desarrollados dentro de la teoría de las restricciones los cuales se fortalecen con el análisis del ciclo de vida, técnicas estadísticas y métodos multicriterios discretos. Los resultados del diagnóstico demostraron la necesidad de redise ̃ nar las decisiones estratégicas teniendo en cuenta los criterios económicos integrados con los medioambientales con el fin de lograr un desempe ̃ no sostenible de la cadena de suministros estudiada.

  19. A comparison of ELISA methods for the determination of human serum antibodies to Haemophilus influenzae type b

    NARCIS (Netherlands)

    van Gageldonk PGM; Mariani M; Berbers GAM; LVO-BI; Centro Ricerche; CHIRON S.p.A.; Siena; Italy/Laboratorio di Immunochimica e Sierologia Sperimentale/Departimento Immunologia

    1998-01-01

    Een vergelijking tussen de non-competitieve ELISA van Phipps et al., de klassieke radio-antigeen bindings assay (RABA) en de nieuw ontwikkelde Chiron competitieve ELISA voor de bepaling van anti-Hib polysaccharide (HibPs) antistof concentraties in humane sera wordt beschreven in het rapport. Voor

  20. Implicaciones de la zona Ossa-Morena y dominios equivalentes en el modelo geodinámico de la Cadena Hercinica europea

    Directory of Open Access Journals (Sweden)

    Castro, A.

    1987-08-01

    Full Text Available The Ossa-Morena zone (OMZ has some peculiar features clearly different from those of the other inner domains in the Iberic Hercynian chain. It is a continental domain (microcontinent ? attached, from Lower Paleozoic times, to the continental crust of the Central Iberian zone (CIZ. During the Hercynian collision (Upper Paleozoic the OMZ behaved in a way different form that of adjacent areas (CIZ due to the peculiar features (Iess thicknees and pre-Hercynian evolution (Ordovician rifting. The OMZ, and their equivalent domains in the Iberian peninsula as well as in European chain, can have strong implications on the evolution of the Hercynian orogene. Part of the OMZ crust (and equivalent domains, together with the oceanic crust developed within it during the Ordovician rifting, was obduced over the inner domains (CIZ in areas of frontal collision (e.g. NW Iberian peninsula and French Massif Central and strongly stretched in areas of oblique collision (e.g. OMZ of SW Iberian peninsula and North Armorican domains.La zona Ossa-Morena (ZOM del hercínico peninsular posee unas características peculiares que la diferencian netamente de los otros dominios internos de la Cadena. Se trata de un dominio cortical (microcontinente ? adosado a la corteza de la Zona Centro-Ibérica (ZCI desde el Paleozoico Inferior. Durante la colisión continental hercínica (Palezoico Superior la ZOM responde de diferente forma que las zonas adyacentes (ZCI debido a su diferente constitución (menor espesor y peculiar evolución prehercínica (rifting ordovícico. Esta zona y sus equivalentes en otros dominios del hercínico peninsular y europeo, tienen fuertes implicaciones en el desarrollo del orógeno hercínico. Parte de la corteza continental de la ZOM fue, junto con la corteza oceánica desarrollada localmente en su interior durante el rifting ordovícido, obducida sobre los dominios internos (ZCI en zonas de colisión frontal (e.g. NW peninsular y Macizo C

  1. Matrix effects in applying mono- and polyclonal ELISA systems to the analysis of weathered oils in contaminated soil.

    Science.gov (United States)

    Pollard, S J T; Farmer, J G; Knight, D M; Young, P J

    2002-01-01

    Commercial mono- and polyclonal enzyme-linked immunosorbent assay (ELISA) systems were applied to the on-site analysis of weathered hydrocarbon-contaminated soils at a former integrated steelworks. Comparisons were made between concentrations of solvent extractable matter (SEM) determined gravimetrically by Soxhlet (dichloromethane) extraction and those estimated immunologically by ELISA determination over a concentration range of 2000-330,000 mg SEM/kg soil dry weight. Both ELISA systems tinder-reported for the more weathered soil samples. Results suggest this is due to matrix effects in the sample rather than any inherent bias in the ELISA systems and it is concluded that, for weathered hydrocarbons typical of steelworks and coke production sites, the use of ELISA requires careful consideration as a field technique. Consideration of the target analyte relative to the composition of the hydrocarbon waste encountered appears critical. PMID:11858166

  2. Development of an ELISA to detect clenbuterol in swine products using a new approach for hapten design.

    Science.gov (United States)

    Bui, Quoc Anh; Vu, Thi Huynh Han; Ngo, Vo Ke Thanh; Kennedy, Ivan R; Lee, N Alice; Allan, Robin

    2016-09-01

    This research outlines the application of an enzyme-linked immunosorbent assay (ELISA) for the analysis of clenbuterol in animal products. Our assay showed good sensitivity for clenbuterol (0.4 ng/g or 0.4 ppb) and low detection limit (0.09 ng/g or 0.09 ppb). A low cross-reactivity for other β2-agonist drugs such as salbutamol, terbutaline, and epinephrine led to formatting an ELISA kit considered to have a high specificity for clenbuterol. A survey of Ho Chi Minh City pork market was conducted as part of the validation of our ELISA. ELISA results showed a surprisingly high value of contamination. However, it will be necessary to conduct a more statistically valid replicated survey with evaluation by other instrumental methods to obtain a definite conclusion. This ELISA kit will be used to monitor growth promoter residues in Vietnam's animal products. PMID:27481170

  3. Screening for protein-DNA interactions by automatable DNA-protein interaction ELISA.

    Directory of Open Access Journals (Sweden)

    Luise H Brand

    Full Text Available DNA-binding proteins (DBPs, such as transcription factors, constitute about 10% of the protein-coding genes in eukaryotic genomes and play pivotal roles in the regulation of chromatin structure and gene expression by binding to short stretches of DNA. Despite their number and importance, only for a minor portion of DBPs the binding sequence had been disclosed. Methods that allow the de novo identification of DNA-binding motifs of known DBPs, such as protein binding microarray technology or SELEX, are not yet suited for high-throughput and automation. To close this gap, we report an automatable DNA-protein-interaction (DPI-ELISA screen of an optimized double-stranded DNA (dsDNA probe library that allows the high-throughput identification of hexanucleotide DNA-binding motifs. In contrast to other methods, this DPI-ELISA screen can be performed manually or with standard laboratory automation. Furthermore, output evaluation does not require extensive computational analysis to derive a binding consensus. We could show that the DPI-ELISA screen disclosed the full spectrum of binding preferences for a given DBP. As an example, AtWRKY11 was used to demonstrate that the automated DPI-ELISA screen revealed the entire range of in vitro binding preferences. In addition, protein extracts of AtbZIP63 and the DNA-binding domain of AtWRKY33 were analyzed, which led to a refinement of their known DNA-binding consensi. Finally, we performed a DPI-ELISA screen to disclose the DNA-binding consensus of a yet uncharacterized putative DBP, AtTIFY1. A palindromic TGATCA-consensus was uncovered and we could show that the GATC-core is compulsory for AtTIFY1 binding. This specific interaction between AtTIFY1 and its DNA-binding motif was confirmed by in vivo plant one-hybrid assays in protoplasts. Thus, the value and applicability of the DPI-ELISA screen for de novo binding site identification of DBPs, also under automatized conditions, is a promising approach for a

  4. Desarrollo de la lectura mediante estratégias integradoras

    Directory of Open Access Journals (Sweden)

    Solé, Maira

    2005-06-01

    Full Text Available La lectura y la escritura son procesos que cada día ameritan nuevos cambios y transformaciones. La propuesta de un Proyecto Pedagógico Integrador, (Fraca 2003 desarrollado con éxito en algunas instituciones venezolanas, se perfila como una alternativa significativa para el desarrollo de estos elementos. La idea o núcleo central es la integración de las diferentes asignaturas curriculares y lograr una globalización partiendo de sus objetivos y contenidos programáticos. El eje pedagógico integrador le permite al docente, evidenciar con mayor prontitud los resultados mediante actividades prácticas de lectura y escritura. Así mismo combina elementos claves del aprendizaje ausbeliano: información previa, información nueva y construcción de la información definitiva o integrada. La puesta en ejecución de las estrategias integradoras, en esta ocasión por maestros en formación (UNEG, a diferentes niños de escuelas del Estado Bolívar (Venezuela, certificando cómo la lectura y la escritura pueden tener un espacio ideal y significativo en la instrucción actual. Solo se necesita la intención, creatividad, dinamismo e ingenio. The reading and the writing plows processes that every day they require new changes and transformations. The proposal of an Integrative Pedagogic Project, (Fraca 2003 developed with success in some Venezuelan institutions; it is profiled like a significant alternative for the development of these elements. The idea or central nucleus is the integration of the different curricular subjects and to achieve a globalization leaving of its objectives and programmatic contents. The integrative pedagogic axis allows to the educational one, to evidence with more readiness the results by means of practical activities of reading and it notarizes. Likewise it combines key elements of the learning ausbeliano: previous information, new information and construction of the definitive or integrated information. The operation of

  5. de teorías mediante un estudio de caso

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    Verónica Alonso Jiménez

    2008-01-01

    Full Text Available La democracia representativa como forma de gobierno, implica que el poder se ejerce por personas, que elegidas por el pueblo, actúan en su nombre y representación. El modo de participación en la elección y la manera como éstas se convierten en cargos públicos, requiere del dise- ño de instituciones que sistematicen dicha participación. La república de tipo presidencial, es una modalidad del gobierno electivo y popular, cuyo titular es el jefe del ejecutivo, electo por el pueblo o sus representantes, en donde el ejercicio del poder es limitado y mantiene un régimen de responsabilidades políticas. El diseño institucional que le corresponde a esta forma de gobierno es la parcelación del poder pú- blico en tres: poder ejecutivo, poder legislativo y poder judicial. Las ventajas políticas de este esquema es que la división de poderes, neutraliza el riesgo de caer en el autoritarismo, al impedir que el poder se concentre. La división de poderes es un dispositivo de restricción de facultades de los órganos estatales, por lo que no existe superioridad jerárquica entre los poderes, al contrario, cada órgano tiene bien delimitadas sus funciones y atribuciones, las que están reguladas por un marco jurídico común llamado Constitución. Y la división de poderes contribuye a mantener el equilibrio entre estos, mediante el llamado sistema de “pesos y contrapesos”. El presente trabajo se centra en el estudio de la Cámara de Diputados, considerada como una de las parcelas en las que está divida la autoridad del Estado, cuyo objetivo es la validación de los modelos teóricos en la tipificación del Congreso y la comprensión de las variables internas y externas que influyen en el comportamiento legislativo.

  6. Metodología basada en el Modelo de Referencia para Cadenas de Suministro para Analizar el Proceso de producción de Biodiesel a partir de Higuerilla Methodology based on the Supply Chain Operations Reference Model to Analyze the Production Process of Biodiesel from Castor Oil Plant

    Directory of Open Access Journals (Sweden)

    Fernando Salazar

    2012-01-01

    Full Text Available Se presenta un análisis para la producción de Biodiesel a partir de Higuerilla usando el Modelo de Referencia para Cadenas de Suministro (SCOR. Se identificaron los niveles del proceso, indicadores clave de desempeño, atributos y operaciones logísticas que se llevan a cabo en toda la cadena de la producción de biodiesel de higuerilla. La utilidad de la aplicación del modelo SCOR se determina por la identificación que logra de los componentes de la cadena, evidenciando fortalezas y debilidades que presenta en las relaciones externas e internas de la logística. Desde esta perspectiva se observó que la aplicación del modelo SCOR hace más eficiente las operaciones logísticas a lo largo de la cadena.The production process of Biodiesel from castor oil plants is analyzed using the Supply Chain Operations Reference Model (SCOR. The different levels of the process, the key performance indicators, the attributes and the logistic operations carried out throughout the chain were analyzed. The usefulness of applying the SCOR model is determined by the identification of the components of the chain, highlighting strengths and weaknesses of external and internal relations of the logistics. From this perspective it was observed that the implementation of the SCOR model makes more efficient the logistics operations through the whole chain.

  7. Evaluation of antigen and antibody ELISA's for epidemiological surveys of tsetse-transmitted trypanosomosis in cattle

    International Nuclear Information System (INIS)

    Sensitivity and specificity of the FAO/IAEA antigen-detection enzyme-linked immunosorbent assay (ELISA) kits for diagnosis of bovine trypanosomosis were investigated using sera from experimental cattle infected by tsetse challenge with cloned populations of Trypanosoma congolense (3 populations) or T. vivax (1 population). The kits are based on monoclonal antibodies that recognise internal antigens of tsetse-transmitted trypanosomes. Ten cattle were infected with each trypanosome population for at least 60 days, and in combination with uninfected cohorts (n=16) were used in a double-blind study design. Sensitivity and specificity of the tests depended on the choice of positive-negative thresholds expressed as percent positivity with respect to the median OD of 4 replicates of the strong positive reference serum provided with the kit. In general, while overall specificities were high, sensitivities of the antigen-ELISA's were poor. For example, at a cut-off of 5% positivity, the sensitivities of the antigen-ELISA's were 11% for samples (n=1162) from T. congolense infected cattle (n=30), and 24% for samples (n=283) from T. vivax infected cattle (n=10). The corresponding specificity values were 95% and 79%, respectively. There were no values of the positive-negative threshold at which both sensitivity and specificity were satisfactory. Trypanosome species-specificities of the antigen-ELISA's were also poor. Sensitivity and species-specificity of the antigen-ELISA for T. brucei infections were not investigated. The indirect ELISA for the detection of anti-trypanosomal antibodies in bovine serum was adapted for use with dried blood spots on filter paper, and standardised using a strong positive reference serum and the percent positivity system of data expression. The antibody-ELISA was evaluated in Zambia for use in epidemiological surveys of the prevalence of tsetse-transmitted bovine trypanosomosis. Known negative samples (sera, n=209; blood spots, n=466) were

  8. Agricultural production - Phase 2. Indonesia. National training course on ELISA for seradiagnosis of animal diseases (4)

    International Nuclear Information System (INIS)

    This report details and UNDP/FAO/IAEA consultancy undertaken from Monday 13 February to Saturday 25 February 1989. The purpose of the consultancy was to provide practical and theoretical training to Indonesian scientists in ELISA technology. This occurred under the program title of ''National Training Course on the Use of ELISA for Serodiagnosis of Animal Diseases, with Emphasis on Brucellosis''. The course was held in the Bacteriology Department, Research Institute for Veterinary Sciences (Balitvet), Bogor, Indonesia. The majority of the 19 participants came from the Regional Disease Investigation Centre Laboratories within Indonesia. The principal course instructor was Dr. Richard Jacobson who was assisted by Dr. Larry McClure, Dr. Susan Sutherland, Dr. Mark Eisler, Dr. Barry Patten and myself. The course concluded with a one day seminar organized by BATAN, DITKESWAN and BALITVET entitled ''Bovine Brucellosis: A Challenging Disease for Indonesia'' which was attended by approximately fifty people. Refs and tabs

  9. FACTIBILIDAD DEL USO DE UN ELISA INDIRECTO PARA LA DETECCIÓN DE Glomus clarum

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    I. de la Providencia

    2004-01-01

    Full Text Available Este trabajo tuvo como objetivo evaluar la factibilidad del uso de un ensayo inmunoenzimático sobre soporte sólido (ELISA, para la detección de esporas de Glomus clarum. Para ello se inmunizaron conejos de la raza Chinchilla con extractos proteicos provenientes de dichas esporas. El suero policlonal obtenido de los animales inmunizados se enfrentó a G. clarum y a las esporas de otras especies de Glomales. Se logró detectar las esporas de G. clarum con el uso del ELISA indirecto a la dilución 1/3 000, sin reacciones cruzadas con las otras especies de Glomales utilizadas en este estudio a esa misma dilución.

  10. The use of polyvinyl alcohol glutaraldehyde as solid-phase in ELISA for plague

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    Aureci Maria Araujo

    1996-04-01

    Full Text Available Discs of polyvinyl alcohol cross-linked with glutaraldehyde were synthesized under acid catalysis (H2SO4. Then, the antigen F1 purified from Yersinia pestis was covalently linked to this modified polymer. Afterwards, an enzyme-linked immunosorbent assay (ELISA was established for the diagnosis of plague in rabbit and human. The best conditions for the method were achieved by using 1.3 ¼g of F1 prepared in 0.067 M phosphate buffer, pH 7.2, containing 1 M NaCl (PBS; anti-IgG peroxidase conjugate diluted 6,000 times and as a blocking agent 3% w/v skim milk in PBS. The titration of positive rabbit serum according to this procedure detected antibody concentrations up to 1:12,800 times. The present method, the conventional ELISA and passive haemagglutination assay are compared.

  11. ELISA - The method of choice for assay of antibodies in sera of livestock

    International Nuclear Information System (INIS)

    Q fever, a coxiellosis, in animals is a worldwide zoonosis caused by Coxiella burnetii. Domestic ruminants - sheep, goats and cattle- are the main reservoirs of C. burnetii responsible for both human and livestock infections. Serology remains the major means of confirmation of clinically suspect C. burnetii infection as well as for seroepidemiological studies. A sandwich enzyme linked immunosorbent assay (ELISA) has been used to detect antibodies in different animal sera and was compared with three commonly used serological methods. Six antigenic preparations obtained by different methods from C. burnetii strain Nine Mile in phases I and II have also been compared. Our results indicate that the detection of antibodies to C. burnetii can be influenced not only by different antigenic preparations, but also by different serological methods. The advantageous sandwich ELISA with its simplicity, automation and objective reading represents the method of choice for screening great numbers of samples and for evaluating epizotoological sitiuations

  12. LDRD final report on microencapsulated immunoreagents for development of one-step ELISA

    Energy Technology Data Exchange (ETDEWEB)

    Henderson, C.C.; Singh, A.K.

    1997-08-01

    Microencapsulation of biological macromolecules was investigated as a method for incorporating the necessary immunoreagents into an improved enzyme-linked immunosorbant assay (ELISA) package that would self-develop. This self-contained ELISA package would eliminate the need for a trained technician to perform multiple additions of immunoreagent to the assay. Microencapsulation by insolution drying was selected from the many available microencapsulation methods, and two satisfactory procedures for microencapsulation of proteins were established. The stability and potential for rapid release of protein from these microencapsulates was then evaluated. The results suggest that the chosen method for protein entrapment produces microcapsules with a considerable amount of protein in the walls making these particular microcapsules unsuitable for their intended use.

  13. Isoelectric focusing and ELISA for detecting adulteration of donkey milk with cow milk.

    Science.gov (United States)

    Pizzano, Rosa; Salimei, Elisabetta

    2014-06-25

    Donkey milk has been recently revalued intensely due to its nutritional properties. Moreover, donkey milk has been proposed as an effective alternative food for some infants with cow milk allergy. Two fast analytical methods were proposed to detect the fraudulent practice of blending cow milk to donkey milk. Detection of cow αs1-casein bands along the profiles of experimental donkey-cow milk mixtures analyzed by isoelectric focusing was adequate to estimate cow milk used as adulterant of donkey milk starting from 5% (v/v). An ELISA-based method using the antipeptide antibodies raised against the 1-28 sequence stretch of cow β-casein was also developed for an accurate definition of composition of donkey-cow milk mixtures. The presence of cow milk at levels as low as 0.5% (v/v) was detected in donkey-cow milk mixtures prepared at laboratory scale and assayed by ELISA. PMID:24892189

  14. ELISA to study antibody responses to anthrax vaccine in cattle, sheep and goats

    International Nuclear Information System (INIS)

    An ELISA based on tri-partite toxin partially purified from Bacillus anthracis was used to determine the antibody responses of groups of cattle, sheep and goats after anthrax vaccination. All species produced detectable increases in antibody titres after vaccination with the order being cattle>sheep>goats. A second vaccination induced variable anamnestic responses depending on the species or time (6 or 22 weeks) after the primary dose. Large differences were observed between individual animals with respect to the antibody induced by the 2 vaccine doses. This observation, together with differences in vaccine quality and apparent immunogenicity may affect efficient control of anthrax outbreaks. ELISA provided a convenient method of determining the relative contribution of these factors to the protection afforded by anthrax vaccination programs. (author). 10 refs, 3 figs, 2 tabs

  15. Detecting polysaccharide antigen of Neisseria meningitidis group C in cerebrospinal fluid by dot-ELISA assay.

    Science.gov (United States)

    Correia Barbosa, S F; Alkmin, M G; Landgraf, I M

    2000-06-01

    Cerebrospinal fluid (CSF) samples from 210 patients (200 with clinical evidence of bacterial meningitis, 10 with other clinical neurologic disease) were tested by a Dot-ELISA assay for detection of polysaccharide antigen of N. meningitidis group C. CSF samples were treated with EDTA 0.1 M, at pH 7.5 and heated to 90>C for 10 min. Polyclonal antiserum was purified by use of ethanol fractionation. The results were compared to those using bacterial culture (BC), latex agglutination (LA), counterimmunoelectrophoresis (CIE), and direct microscopy (DM) methods. Test results showed a correlation of 93.3%, 94.3%, 91.0% and 69.5% respectively, and sensitivity of 0.947 and specificity of 0.930. This study suggests that the dot-ELISA assay of CSF is a useful alternative technique for the diagnosis of group C meningitis. PMID:10934498

  16. Detection of Epstein- Barr virus infection in lymphoma: ELISA and PCR method

    Directory of Open Access Journals (Sweden)

    Pourakbari B

    2010-02-01

    Full Text Available "n Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Arial; mso-bidi-theme-font:minor-bidi;} Background: Latent Epstein- Barr virus (EBV genomes are found in the malignant cells of approximately one-third of Hodgkin's lymphoma (HL cases. Detection of EBV viral DNA could potentially be used as a biomarker of disease activity. Our goal was to compare of EBV DNA detection in samples obtained from lymphoma patients versus controls."n"nMethods: One milliliter uncoagulated and 1ml coagulated blood sample for DNA extraction and serum analysis using ELISA for IgG anti EBNA-1 were obtained from 44 lymphoma patients and from 44 normal controls, respectively. EBV genome, EBNA-2, was examined from DNA extracts of paraffin embedded and blood samples using Nested PCR with type specific inner primers."n"nResults: Positive results for ELISA, Blood and biopsy PCR in study group were, 84.1%, 27.3% and 13.6%, respectively. However, these results in control group were 47.7% and 16% for ELISA and Blood PCR assays, respectively. Positive results in ELISA, Blood PCR and Biopsy PCR in Hodgkin and non-Hodgkin patients were found in 21(84%, 6(24%, 4(16% and 16(84.2%, 6(31.6%, 2(10.5% of specimens, respectively. No significant differences in EBV detection were found between these two patient groups (p values for ELISA, Blood PCR

  17. Preparation and Characteristic of Dextran-BSA Antibody and Establishment of it’s Elisa Immunoassay

    OpenAIRE

    Zhen-ming X; Li-sha F; Da-feng L; Lin Y

    2014-01-01

    The enzyme linked immunosorbent assay (ELISA) is a potential tool for the determination of dextran. In this study, dextran–BSA antigens were prepared by Reductive amination method, and were confirmed by SDS-PAGE and free amino detection. The effects of coupled reaction conditions such as different oxidation degree of dextran, the reaction time were investigated and the immunity of the resulting dextran- BSA neoglycoprotein antigens were evaluated through the interaction w...

  18. A Comparison of Immuncapture Agglutination and ELISA Methods in Serological Diagnosis of Brucellosis

    Directory of Open Access Journals (Sweden)

    Mehmet Özdemir, Bahadır Feyzioğlu, Muhammed Güzel Kurtoğlu, Metin Doğan, Hatice Türk Dağı, Şerife Yüksekkaya, Recep Keşli, Bülent Baysal

    2011-01-01

    Full Text Available Background: Different serological tests are used in serologic diagnosis of brucellosis. The most widely used of these are Standard Tube Agglutination and Coombs anti-brucella tests. Whereas ELISA Ig M and Ig G tests have been in use for a long time, immuncapture agglutination test has been recently introduced and used in serological diagnosis. The aim of this study was to compare diagnostic values of ELISA Ig M and Ig G and immuncapture agglutination tests with Coombs anti-brucella test.Methods: Sera from 200 patients with presumptive diagnosis of brucellosis were included into the study. Coombs anti-brucella test, ELISA Ig M and Ig G tests and Immuncapture test were investigated in these sera. Then, sensitivity, specificity, negative predictive and positive predictive values were calculated.Results: Sensitivity, specificity, negative predictive and positive predictive values were found to be 90,6 %, 76,3 %, 94,2 %, and 65,9 % respectively for the Immuncapture test, whereas they were found to be 73,7 %, 58,9 %, 84,2 %, and 42,8 % for Ig G and 72,2 %, 67,8 %, 85,2 %, and 48,7 % for Ig M. The Immuncapture test was found to be compatible with ELISA Ig M and Ig G tests but it was statistically incompatible with Coombs anti-brucella test.Conclusions: Immuncapture agglutination test yields similar results to those of Coombs anti-brucella test. This test is a useful test by virtue of the fact that it determines blocking antibodies in the diagnosis and follow-up of brucellosis.

  19. Conglutinating complex fixation test (CCFR) as a method alternate to enzyme linked immunosorbent assay (ELISA)

    International Nuclear Information System (INIS)

    An enzyme linked immunosorbent assay (ELISA) has proved to be the most versatile and sensitive method for the serological diagnosis of infectious animal diseases. However, other assays offer similar advantages. This has been shown by our research through the development of a conglutinating complex fixation test (CCFT). This is analogous to the complement fixation test (CFT) and is based on the phenomenon of conglutination. The limiting factor in this test is the source in cattle blood

  20. Development of sandwich ELISAs for the detection of aromatic diisocyanate adducts

    OpenAIRE

    Lemons, Angela R.; Bledsoe, Toni A.; Siegel, Paul D.; Beezhold, Donald H.; Brett J Green

    2013-01-01

    Diisocyanates (dNCOs) are highly reactive low molecular weight chemicals commonly used in the manufacturing industry. Occupational exposures to dNCOs have been shown to elicit allergic sensitization and occupational asthma. Among the most commonly used dNCOs in industry are the aromatic dNCOs, toluene diisocyanate (TDI) and methylene diphenyl diisocyanate (MDI). This study aimed to develop enzyme linked immunosorbent assays (ELISA) utilizing aromatic dNCO-specific monoclonal antibodies (mAbs)...

  1. Detection of Foot-and-mouth Disease Serotype O by ELISA Using a Monoclonal Antibody

    OpenAIRE

    Chen, Hao-tai; Peng, Yun-hua; ZHANG, YONG-GUANG; Liu, Xiang-tao

    2012-01-01

    An ELISA assay with monoclonal antibody (MELISA) was used to type serotype O of foot-and-mouth disease virus (FMDV). All FMDV serotype O reference strains were positive by MELISA, while other viruses such as FMDV serotypes Asia 1, C, and A and classical swine fever virus, swine vesicular disease virus, and porcine reproductive and respiratory syndrome virus remained negative. Furthermore, FMDV serotype O positive samples were able to be detected by MELISA. This assay may be particularly suita...

  2. A Leishmania infantum FML-ELISA for the Detection of Symptomatic and

    Directory of Open Access Journals (Sweden)

    Behnam Mohammadi-Ghalehbin

    2011-12-01

    Full Text Available Background: Visceral leishmaniasis (VL is caused by Leishmania infantum in Mediterraneanbasin and is an endemic disease in some parts of Iran. Canines are the mainreservoirs of VL in most of the endemic areas. Different serological methods have beenintroduced for diagnosis of canine visceral leishmaniasis (CVL. Objective: In this surveya Fucose-Mannose Ligand (FML ELISA, using native L. infantum antigen, wasdeveloped and its validity for detection of infected dogs in comparison with direct agglutinationtest (DAT and PCR was evaluated. Methods: Blood samples of sixty ownershipdogs (≤ 3 years old were collected from Meshkin-shahr district in Ardabil province,North-west of Iran. Sera were separated for serological assays (DAT and FMLELISAand the buffy coats were collected for molecular evaluation. Results: Two outof the 60 (3.33% samples were found to be positive (antibody titer of ≥ 1/320 in DATwhile seven of the 60 (11.66% samples were positive by FML-ELISA. Nine out of 60(15% buffy coat samples showed a band about 680 bp indicative of L. infantum inPCR. Three out of 60 dogs had Kala-azar symptoms and were positive by PCR andFML-ELISA, while two of these three dogs had antibody titers >1/320 in their serumsamples. The sensitivity and specificity of FML-ELISA for the detection of CVL inboth symptomatic and asymptomatic dogs were found to be 77.8% and 100%, respectively.Conclusion: Considering the acceptable sensitivity and high specificity of FMLELISA,use of this serological method can be recommended for epidemiological surveysof CVL.

  3. Biotyping of Clostridium Perfringens strains isolated from enterotoxemia cases in sheep using ELISA technique

    OpenAIRE

    M. A. Hamad; N. Habra; A. Kalb Allouz3

    2010-01-01

    The study was designed to determine the types of Clostridium perfringens and their toxins in sheep with suspected enterotoxemia in Hama province, Syria. For this purpose, 89 bacterial isolates were isolated from 132 samples collected from dead and some slaughtered sheep with suspected enterotoxemia and diagnosed with classical diagnostic tests and by using enzyme-linked immunosorbent assay (ELISA) technique to determine the types and toxins of C. perfringens. These isolates appeared as G+ bul...

  4. Toxinotyping of Clostridium perfringens isolates by ELISA and PCR from lambs suspected of enterotoxemia

    OpenAIRE

    HADİMLİ*, Hasan Hüseyin; ERGANİŞ, Osman; SAYIN, Zafer; ARAS, Zeki

    2012-01-01

    Clostridium perfringens is a gram-positive, anaerobic bacterium that causes a wide range of diseases in humans and animals. The purposes of this study were to determine, using the enzyme-linked immunosorbent assay (ELISA), the clostridial toxins in intestinal samples of lambs with suspected enterotoxemia; to conduct molecular typing of C. perfringens isolates; and to investigate the presence of the genes of 4 major toxins (a, b, e, and i) in the isolates by polymerase chain reaction (PCR). Ac...

  5. High specificity of a novel Zika virus ELISA in European patients after exposure to different flaviviruses.

    Science.gov (United States)

    Huzly, Daniela; Hanselmann, Ingeborg; Schmidt-Chanasit, Jonas; Panning, Marcus

    2016-04-21

    The current Zika virus (ZIKV) epidemic in the Americas caused an increase in diagnostic requests in European countries. Here we demonstrate high specificity of the Euroimmun anti-ZIKV IgG and IgM ELISA tests using putative cross-reacting sera of European patients with antibodies against tick-borne encephalitis virus, dengue virus, yellow fever virus and hepatitis C virus. This test may aid in counselling European travellers returning from regions where ZIKV is endemic. PMID:27126052

  6. Automated type specific ELISA probe detection of amplified NS3 gene products of dengue viruses.

    OpenAIRE

    Chow, V T; Yong, R Y; Ngoh, B L; Chan, Y. C.

    1997-01-01

    AIM: To apply an automated system of nucleic acid hybridisation coupled with the enzyme linked immunosorbent assay (ELISA) for the type specific detection of amplification products of dengue viruses. METHODS: Non-structural 3 (NS3) gene targets of reference strains of all four dengue and other flaviviruses, as well as dengue patient viraemic sera, were subjected to reverse transcription and polymerase chain reaction using consensus and dengue type specific primers and digoxigenin-11-dUTP labe...

  7. Validation of antibody ELISA for use in combination with parasitological tests in monitoring trypanosomosis control programmes

    International Nuclear Information System (INIS)

    The available techniques for trypanosomosis diagnosis, surveillance and control have failed to provide an effective control of the disease when used in isolation. However, when used in combination the techniques might provide a better alternative for a sustainable disease control strategy. Antigen-detection ELISA was validated and established at ADRI through support of the Joint FAO/IAEA Division, but still required perfection in order to meet the requirements for which it was intended (detection of current infection and species specificity). The primary objective of the present study was to evaluate the trypanosomal antibody-detection ELISA (Ab-ELISA) kit produced by the International Atomic Energy Agency (IAEA) Vienna, Austria, to supplement conventional diagnostic techniques in trypanosomosis surveillance and control. Three-hundred sixty cattle from selected herds in the Tanga region of Tanzania were bled to obtain buffy-coat and serum samples in a study of the prevalence of trypanosomosis using parasitological and serological techniques. The prevalence of trypanosomosis, by means of parasitological techniques, in Korogwe, Muheza, Tanga and Pangani districts of Tanga region, varied from 0-18.2%. The IAEA Ab-ELISA kit, showed a 97.2% specificity for Trypanosoma congolense and T. vivax antibodies, at a cut-off point of 30.0% positivity (PP), out of 160 sera from a trypanosomosis-negative population; and a 94.5% sensitivity for the same antibodies and cut-off point, out of 200 sera from trypanosomosis-buffy-coat-positive samples. Packed red cell volume (PCV) values for a trypanosomosis-negative population were much higher than those from cattle from trypanosomosis-endemic areas: 77.5% of samples from a disease-free area showed PCV values higher than 30.0, while only 15% of the samples from endemic areas were above 30.0. (author)

  8. Crude antigens of Fasciola hepatica and Fasciola gigantica using ELISA test: a comparative study

    Directory of Open Access Journals (Sweden)

    Gaur S.N.S

    2008-05-01

    Full Text Available Background: Fasciolosis is a worldwide disease with major economic and public health consequences. Early detection of the infection is important for the prevention and control of the disease. ELISA allows for early detection of fasciolosis in man and animals. Fasciolosis is caused by Fasciola hepatica and F. gigantica in man and domestic animals respectively. These two species have many similar morphological characteristics. In this study, the crude antigens of these two species are investigated by ELISA test. Methods: The excretory-secretory and somatic antigens of two species were prepared from adult flukes collected from the bile ducts of sheep and stored at -20oC. For the preparation of the antisera, the antigens were injected to laboratory-bred rabbits. Each rabbit received five injections at intervals of seven days, starting with 0.5 ml and ending with 2.5 ml. Ten days after the last injection, the rabbits were bled, and serum samples separated and stored at -20oC. The reaction between homologous and heterologous antigens and antisera was tested by ELISA and optical densities were recorded.Results: Excretory- secretory and somatic antigens of each species showed a strong positive reaction with the antisera of the other species. In a homologous combination of antigens and antisera, a stronger reaction was observed compared to the heterologous combination, therefore many antigenic materials of both species are the same.Conclusion: The differences of these crude antigenic materials of F. hepatica and F. gigantica are insufficient to prevent cross reaction of two species by ELISA. Further investigations are recommended for the identification, detection and purification of antigenic material of each species to improve the specificity of this assay.

  9. PrPSc detection in formalin-fixed paraffin-embedded tissue by ELISA

    OpenAIRE

    Eric M Nicholson; Greenlee, Justin J; Hamir, Amir N

    2011-01-01

    Background Formalin-fixed paraffin-embedded tissue is regularly employed in the diagnosis of transmissible spongiform encephalopathies (TSE) by immunohistochemistry (IHC), the standard by which all other TSE diagnostic protocols are judged. While IHC affords advantages over diagnostic approaches that typically utilize fresh or frozen tissue, such as Western blot and ELISA, the process of fixing, staining, and analyzing individual sections by hand does not allow for rapid or high throughput sc...

  10. Penggunaan ELISA untuk Mendeteksi Cucumber Mosaic Virus dan Tobacco Mosaic Virus pada Tanaman Cabai

    OpenAIRE

    Taufik, Muhammad; Khaeruni, Andi; Rombe, Wau S.L.

    2011-01-01

    This study was conducted at the Laboratory of Plant Pests and Diseases, Faculty of Agriculture, Haluoleo University Kendari, Southeast Sulawesi, from November 2010 to January 2011. The objective of this experiment was to detect the presence of cucumber mosaic virus (CMV) and tobacco mosaic virus (TMV) on pepper in Southeast Sulawesi by using an ELISA (enzyme-linked immunosorbent assay) technique. A survey was conducted to collect chili leaf samples in several locations in the Prov...

  11. Investigation of Entamoeba histolytica in stool specimens by ELISA during a year

    OpenAIRE

    Dal, Tuba

    2011-01-01

    Objectives: The aim of this study was to investigate Entamoeba histolytica in the stool samples that have been sent to microbiology laboratory by ELISA method. Materials and methods: This study was performed on 800 stool specimens belonging to different age groups and different patients sent to Microbiology Laboratory of a State Hospital between January 2009 and December 2009. Results: In this study Entamoeba histolytica/dispar cysts and/or trophozoites were observed in 31 (3.9%) out ...

  12. A novel ELISA for measuring CD36 protein in human adipose tissue

    OpenAIRE

    Allred, Carolyn C.; Krennmayr, Thomas; Koutsari, Christina; Zhou, Lianzhen; Ali, Asem H.; Jensen, Michael D.

    2011-01-01

    CD36 is a transmembrane protein present in many tissues that is believed to facilitate inward fatty acid transport. Western blotting is the most widely used method to measure tissue CD36 protein content, but it is time consuming, technically demanding, and semiquantitative. To more precisely measure adipose tissue CD36 content we developed an enzyme linked immunosorbent assay (ELISA) after establishing that: 1) the anti-CD36 antibodies gave a single distinct band on traditional Western blots,...

  13. Detection of ruminant meat and bone meal in feeds by sandwich ELISA with monoclonal antibodies.

    Science.gov (United States)

    Yamamoto, Takayuki; Kato, Masatoshi; Endo, Kiwamu; Kotoura, Satoshi; Takeda, Zenya

    2016-01-01

    A sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) using two monoclonal antibodies directed against a synthetic peptide with an amino-acid sequence related to the C-terminus of bovine myoglobin and the whole molecule of sodium dodecyl sulphate (SDS)-denatured bovine myoglobin was adapted for detecting bovine myoglobin in contaminated feeds. The ELISA employed bovine meat extract of a known myoglobin concentration as a calibration standard and had an limit of detection (LOD) of 3.54 ng/ml and an limit of quantification (LOQ) of 11.0 ng/ml corresponding to 0.022% and 0.067% (wt/wt) bovine meat-and-bone-meal (MBM) mixed in 20-fold-diluted feed extracts, respectively. A cut-off threshold of 20.6 ng/ml bovine myoglobin was set to simplify ELISA and facilitate quick assessment of test results without a tedious calibration process. The ELISA was able to detect bovine MBM in artificially prepared model feeds, mixed botanical feeds, mixed botanical feeds with skimmed milk, fish meal, pork meal and pork/chicken meal at 0.1% (wt/wt). It was also able to detect sheep MBM in test feeds, but showed no reactivity to swine MBM, chicken MBM, skimmed milk or gelatine of bovine origin. The advantages of this method are the quick and easy extraction protocol of proteins from test feeds, using 100 mM sodium sulphide and 0.6% sodium dodecyl sulphate in the extraction solution and the effective detection of bovine and sheep MBM at 0.1% (wt/wt). PMID:26166832

  14. Detection of Trichinella infection in slaughter horses by artificial digestion, ELISA and PCR

    OpenAIRE

    Viveros N.; Arriaga C.; Banda V.; Ortega-Pierres M.G.; Yépez-Mulia L.

    2001-01-01

    In this study we compared the sensitivity of molecular, serologic and parasitologic methods for diagnosis of equine trichinellosis in two abattoirs, one rural and one federal inspection type. Diaphragm muscle samples were obtained from 170 slaughter horses and examined by artificial digestion and PCR. Serum samples from these horses were also analyzed by ELISA. No Trichinella muscle larvae were detected by artificial digestion. However, specific antibodies against Trichinella were detected in...

  15. Detection of expressed IL-32 in human stomach cancer using ELISA and immunostaining.

    Science.gov (United States)

    Seo, Eun-Hee; Kang, Jeongwoo; Kim, Ki-Hong; Cho, Min-Chul; Lee, Sojung; Kim, Hee-Jong; Kim, Jung-Hee; Kim, Eun-Jin; Park, Dong-Ki; Kim, Soo-Hyun; Choi, Yang Kyu; Kim, Jin Man; Hong, Jin Tae; Yoon, Do-Young

    2008-09-01

    Interleukin (IL)-32 is a recently identified proinflammatory cytokine that is one of the IL-18 inducible genes, and plays an important role in autoimmune and inflammatory diseases. We produced antibodies against IL-32 and studied the expression of IL-32 in human stomach cancer. We detected IL-32 secreted from K-562 cells that werw stably transfected with IL-32 and in the sera of stomach cancer patients, by a sandwich ELISA using a monoclonal antibody KU32-52 and a polyclonal antibody. In order to optimize a sandwich immunoassay, recombinant IL-32alpha was added, followed by the addition of a biotinylated KU32-52 into microtiter plate wells precoated with a goat anti-IL-32 antibody. The bound biotinylated KU32-52 was probed with a streptavidin conjugated to HRP. This sandwich ELISA was highly specific and had a minimal detection limit of 80 pg/ml (mean+/-SD of zero calibrator) and measuring up to 3,000 pg/ml. This ELISA showed no cross-reaction with other cytokines such as hIL-1alpha, hIL-1beta, hIL-2, hIL-6, hIL-8, hIL-10, hIL-18, and hTNF-alpha. Intra-assay coefficients of variation were 18.5% to 4.6% (n=10), and inter-assay coefficients were 23% to 9% (n=10). The average IL-32 level in the sera of 16 stomach cancer patients (189 pg/ml) was higher than that of 12 healthy control men (109 pg/ml). Our results indicate that serum IL-32 level can be detected by using an established ELISA, and that this immunoassay and mAb KU32-09 specific for immunohistochemistry can be used in the detection of expressed and secreted IL- 32 in stomach cancer patients. PMID:18852519

  16. Detection of peste des petits ruminants virus antigen using immunofiltration and antigen-competition ELISA methods.

    Science.gov (United States)

    Raj, G Dhinakar; Rajanathan, T M C; Kumar, C Senthil; Ramathilagam, G; Hiremath, Geetha; Shaila, M S

    2008-06-22

    Peste des petits ruminants (PPR) is one of the most economically important diseases affecting sheep and goats in India. An immunofiltration-based test has been developed using either mono-specific serum/monoclonal antibodies (mAb) prepared against a recombinant truncated nucleocapsid protein of rinderpest virus (RPV) cross-reactive with PPR virus. This method consists of coating ocular swab eluate from suspected animals onto a nitrocellulose membrane housed in a plastic module, which is allowed to react with suitable dilutions of a mAb or a mono-specific polyclonal antibody. The antigen-antibody complex formed on the membrane is then detected by protein A-colloidal gold conjugate, which forms a pink colour. In the immunofiltration test, concordant results were obtained using either PPRV mAb or mono-specific serum. Another test, an antigen-competition ELISA which relies on the competition between plate-coated recombinant truncated 'N' protein of RPV and the PPRV 'N' protein present in ocular swab eluates (sample) for binding to the mono-specific antibody against N protein of RPV (in liquid phase) was developed. The cut-off value for this test was established using reverse transcription polymerase chain reaction (RT-PCR) positive and negative oculo-nasal swab samples. Linear correlation between percent inhibition (PI) values in antigen-competition ELISA and virus infectivity titres was 0.992. Comparison of the immunofiltration test with the antigen-competition ELISA yielded a sensitivity of 80% and specificity of 100%. These two tests can serve as a screening (immunofiltration) and confirmatory (antigen-competition ELISA) test, respectively, in the diagnosis of PPR in sheep or goats. PMID:18182256

  17. Comparison of three ELISA methods with CLIF test for detection of Anti-dsDNA antibody

    OpenAIRE

    Feyza Çetin; Alparslan Toyran; Özlem Aytaç; Feride Alaca Coşkun; İpek Mumcuoğlu; Feyza Alp; Altan Aksoy

    2015-01-01

    INTRODUCTION[|]Systemic lupus erythematosus (SLE) is an autoimmune disease caused by antibodies which produced against antigens commonly on cell nuclei. According to the criteria of American College of Rheumatology (ACR), the immunological parameters which are used for diagnosis of SLE, are anti- nuclear antibodies (ANA) and anti- dsDNA autoantibodies. In this study it is aimed to investigate the specifity of three different ELISA tests by comparing with CLIF test, as ...

  18. The use of ELISAs for monitoring exposure of pig herds to Brachyspira hyodysenteriae

    Directory of Open Access Journals (Sweden)

    Song Yong

    2012-01-01

    Full Text Available Abstract Background Swine dysentery (SD, a mucohaemorrhagic diarrhoeal disease of pigs, results from infection of the large intestine with the spirochaete Brachyspira hyodysenteriae. ELISA systems using whole spirochaete cells (WC and the B. hyodysenteriae outer membrane lipoprotein Bhlp29.7 previously have been established as potential diagnostic tools for SD. However, their true value in identifying infected herds remains unclear. The present study aimed to compare the performance of whole-cell and Bhlp29.7 based ELISAs in detecting specific immunoglobulin class IgG and IgM to B. hyodysenteriae in growing pigs, and additionally evaluated whether meat juice could serve as a source of specific antibodies. Results Levels of circulating IgG and IgM reacting with WC spirochaete preparations and recombinant Bhlp29.7 peaked 4-6 weeks post-infection in the experimentally challenged pigs, and remained elevated in the present study. In a cohort of pigs on an infected farm levels of antibody directed against both antigens showed a progressive increase with time. However, other than for the level of IgG against WC antigen, a significant increase in antibody levels also was observed in a cohort of pigs on a non-infected farm. In addition, assays using meat juice had 100% specificity and equivalent sensitivity to those based on serum, and likewise the best performance was achieved using the WC IgG ELISA. Conclusions IgG ELISAs using either WC or Bhlp29.7 as plate-coating antigens were shown to be useful for monitoring the dynamics of B. hyodysenteriae infection in grower pigs. Of the two antigens, the WC preparation tended to give better discrimination between pigs from infected and non-infected farms. Testing of meat juice was shown to have potential for identifying infected herds.

  19. Multihapten approach leading to a sensitive ELISA with broad cross-reactivity to microcystins and nodularin

    OpenAIRE

    Samdal, Ingunn Anita; Ballot, Andreas; Løvberg, Kjersti Eriksen; Miles, Christopher Owen

    2014-01-01

    Microcystins (MCs) are a group of biotoxins (>150) produced by cyanoba cteria, with a worldwide distribution. MCs are hepatotoxic, and acute exposure causes severe liver damage in humans and animals. Rapid and cheap methods of analysis are therefore required to protect people and livestock, especially in developing countries. To include as many MCs as possible in a single analysis, we developed a new competitive ELISA. Ovine polyclonal antibodies were raised using a...

  20. Evaluation of a blocking ELISA for the detection of antibodies against Lawsonia intracellularis in pig sera

    Directory of Open Access Journals (Sweden)

    Merza Malik

    2011-04-01

    Full Text Available Abstract Background Lawsonia intracellularis is a common cause of chronic diarrhoea and poor performance in young growing pigs. Diagnosis of this obligate intracellular bacterium is based on the demonstration of the microbe or microbial DNA in tissue specimens or faecal samples, or the demonstration of L. intracellularis-specific antibodies in sera. The aim of the present study was to evaluate a blocking ELISA in the detection of serum antibodies to L. intracellularis, by comparison to the previously widely used immunofluorescent antibody test (IFAT. Methods Sera were collected from 176 pigs aged 8-12 weeks originating from 24 herds with or without problems with diarrhoea and poor performance in young growing pigs. Sera were analyzed by the blocking ELISA and by IFAT. Bayesian modelling techniques were used to account for the absence of a gold standard test and the results of the blocking ELISA was modelled against the IFAT test with a "2 dependent tests, 2 populations, no gold standard" model. Results At the finally selected cut-off value of percent inhibition (PI 35, the diagnostic sensitivity of the blocking ELISA was 72% and the diagnostic specificity was 93%. The positive predictive value was 0.82 and the negative predictive value was 0.89, at the observed prevalence of 33.5%. Conclusion The sensitivity and specificity as evaluated by Bayesian statistic techniques differed from that previously reported. Properties of diagnostic tests may well vary between countries, laboratories and among populations of animals. In the absence of a true gold standard, the importance of validating new methods by appropriate statistical methods and with respect to the target population must be emphasized.

  1. Antigen capture ELISA for the heat shock protein (hsp60) of Chlamydia trachomatis.

    OpenAIRE

    Horner, P J; Ali, M.; Parker, D.; Weber, J. N.; Taylor-Robinson, D.; McClure, M O

    1996-01-01

    AIMS: To develop an indirect ELISA using the heat shock protein (hsp60) of Chlamydia trachomatis as antigen. METHODS: The hsp60 gene was amplified by PCR, expressed in the vector pDEV-107 and transformed into Escherichia coli. The recombinant protein, expressed as a beta-galactosidase fusion product, was captured onto a solid phase using a monoclonal antibody directed against beta-galactosidase. Following incubation with goat anti-human antibody conjugated to peroxidase and colour development...

  2. Use of an enzyme-linked immunosorbent assay (Elisa) for the determination of HSP 70

    International Nuclear Information System (INIS)

    It has been suggested that HSP 70 is a good indicator to quantify thermotolerance. The authors developed an enzyme-linked immunosorbent assay (Elisa) for measuring the total amount of HSP 70 in mammalian cells. Microtitration plates were incubated with various concentrations of cell lysate in PBS. Milk proteins were used to block non-specific protein binding sites in the wells. Monoclonal antibody against purified HSP 70 raised in a mouse was added, followed by peroxidase conjugated goat-anti-mouse antibody. After washing, the bound peroxidase activity was measured colorimetrically to calculate the original amount of HSP 70 antigen. Kinetic studies indicate that the linear range of the assay corresponds to the amount of HSP 70 from 103-104 HA-1 cells (0.1-2.0 μg cytosolic proteins in each well). Elisa is a highly sensitive method for quantifying HSP 70 and can be easily used to measure the total amount of HSP 70 instead of the synthesis rate of HSP 70 by pulse radiolabeling. The authors are currently developing the sensitive Elisa system for measurement of thermotolerance in human tumors. A comparison of measurement of HSP 70 by pulse labeling the cells with 35S-methionine and by Elisa has been done in HA-1 cells. HA-1 cells were exposed to 450C, 10 min, then incubated at 370C for 0-24 h. These results show that the rate of HSP synthesis increased several fold, although the total amount of HSP 70 is increased less than 2 fold during thermotolerance induction. These results are consistent with data in the literature obtained by other techniques

  3. ELISA-based assay for IP-10 detection from filter paper samples

    DEFF Research Database (Denmark)

    Drabe, Camilla Heldbjerg; Blauenfeldt, Thomas; Ruhwald, Morten

    2014-01-01

    IP-10 is a small pro-inflammatory chemokine secreted primarily from monocytes and fibroblasts. Alterations in IP-10 levels have been associated with inflammatory conditions including viral and bacterial infections, immune dysfunction, and tumor development. IP-10 is increasingly recognized as a b...... biomarker that predicts severity of various diseases and can be used in the immunodiagnostics of Mycobacterium tuberculosis and cytomegalovirus infection. Here, we describe an ELISA-based method to detect IP-10 from dried blood and plasma spot samples....

  4. Implementation and Setup of a System Dedicated Logbook based on ELisA

    CERN Document Server

    Van Tonder, Raynette

    2016-01-01

    ELisA is a web tool used by the ATLAS community as a daily logbook facility in order to record and share information concerning the experiment's operation and deployment. A few subsystems, notably the FTK subsystem, of the ATLAS experiment would like to setup a logbook based on the ELisA logbook for their own private usage. This new logbook would be completely separated from the main ATLAS logbook and will only be accessed from the general public network. In this project the implementation and setup of the ELisA based logbook will be discussed as well as various modifications that were made to the new logbook. These modifications were specified by the FTK users in order to make the logbook relevant to the FTK subsystem. Once the logbook was functioning as expected and the users were satisfied with the modifications, the FTK logbook was installed on a FTK dedicated machine where it is currently being used by members of the FTK group.

  5. Seroprevalence of Ovine Brucellosis by Modified Rose Bengal Test and ELISA in Southern Punjab, Pakistan

    Directory of Open Access Journals (Sweden)

    Zahid Iqbal, Huma Jamil*, Zafar Iqbal Qureshi, Muhammad Saqib, Laeeq Akbar Lodhi, Muhammad Salman Waqas and Muhammad Safdar

    2013-11-01

    Full Text Available Pertaining to reported high prevalence of brucellosis in Pakistan, district Layyah on account of having thick sheep population was selected for this study. A total of 384 sheep blood samples were collected randomly from different selected private herds in the district, and tested through Modified Rose Bengal (mRB test and Indirect Enzyme Linked Immunosorbent Assay (iELISA for the serological analysis against the Brucella antibodies. Positive samples from these two tests were further subjected to Competitive Enzyme Linked Immunosorbent Assay (cELISA. The individual based seroprevalence of brucellosis in sheep was found to be 7.0% by mRB. Herd based prevalence was 42.5%. The highest (P<0.05 seroprevalence (16.8% was observed in Tehsil Layyah as compared to Tehsils Karor (2.3% and Choubara (4.5%. Non-significant differences were recorded between breeds, age and sex groups and also for the animals with or without history of abortion. In case of indirect and competitive ELISA, no sheep serum sample was found to be positive.

  6. The Efficacy of Multiplex PCR in Comparison with Agglutination and ELISA in

    Directory of Open Access Journals (Sweden)

    Reza Shahrokhabadi

    2014-06-01

    Full Text Available Background: Human brucellosis is an endemic disease in many countries including Iran. Exact diagnosis of brucellosis is not just based on clinical symptoms, because it will be considered in differential diagnosis of other diseases. Therefore, defining organism in culture or identification of organism by serological and molecular methods for confirming clinical diagnosis is necessary. Our aim was to develop a diagnostic PCR assay and define the optimal clinical specimen for this test. Materials and Methods: This cross-sectional and descriptive study was from February 2011 to November 2012. Results of standard agglutination test (SAT and specific immunoglobulin IgG and IgM by enzyme-linked immunosorbent assay (ELISA were compared with multiplex PCR in 116 patients with suspected brucellosis referred to the Ali Ebn-e-Abitaleb hospital, Rafsanjan, Iran. Their sera were collected and tested by SAT, ELISA and multiplex PCR. DNA was extracted from serum samples and examined by multiplex PCR involving specific primers for Brucella melitensis and Brucella abortus based on IS711 in the brucella chromosome. Results: Brucellosis was confirmed in 116 patients (75% male and 25% female based on applied diagnostic methods and clinical features. Results of ELISA, the SAT, and PCR were positive in 116, respectively. B. abortus and B. melitensis were detected in 101 and 15 patients. Conclusion: The results of present study showed that multiplex PCR assay is a rapid and sensitive technique for diagnosis of brucellosis compared to SAT. However it is more accurate when coupled with conventional methods.

  7. [Evaluation of IHA, ELISA and Western Blot tests in diagnosis of pulmonary cystic hidatidosis].

    Science.gov (United States)

    Akisu, Ciler; Bayram Delibaş, Songül; Yuncu, Gökhan; Aksoy, Umit; Ozkoç, Soykan; Biçmen, Can; Sevinç, Serpil; Yaldiz, Sadik

    2005-01-01

    Pulmonary cystic hidatidosis caused by the larval stages of Echinococcus granulosus is a common parasitic disease in Turkey and throughout the world. In this study IHA, ELISA and Western Blot (WB) tests were performed with a panel of 59 sera from 31 surgically confirmed pulmonary cystic hidatidosis patients, 18 patients with pulmonary disease other than cystic hidatidosis and 10 healthy individual. The overall sensitivity of the IHA, ELISA and WB tests used for the serodiagnosis of pulmonary cystic hidatidosis were found as 96.7%, 87.1%, 100% and the specificities were 82.2%, 89.2% and %85.7, respectively. Using the WB test 8-12 kDa, 24 kDa and 124 kDa bands were detected as valuable for surgically confirmed patients' sera. One or more of these bands were also detected in sera of four patients with other pulmonary diseases false-positively. In conclusion conventional serologic test like IHA and ELISA is valuable in diagnosis of pulmonary cystic hidatidosis, also evaluation of some specific bands in WB would contribute to the diagnosis. PMID:16100652

  8. Validation procedures for quantitative gluten ELISA methods: AOAC allergen community guidance and best practices.

    Science.gov (United States)

    Koerner, Terry B; Abbott, Michael; Godefroy, Samuel Benrejeb; Popping, Bert; Yeung, Jupiter M; Diaz-Amigo, Carmen; Roberts, James; Taylor, Steve L; Baumert, Joseph L; Ulberth, Franz; Wehling, Paul; Koehler, Peter

    2013-01-01

    The food allergen analytical community is endeavoring to create harmonized guidelines for the validation of food allergen ELISA methodologies to help protect food-sensitive individuals and promote consumer confidence. This document provides additional guidance to existing method validation publications for quantitative food allergen ELISA methods. The gluten-specific criterion provided in this document is divided into sections for information required by the method developer about the assay and information for the implementation of the multilaboratory validation study. Many of these recommendations and guidance are built upon the widely accepted Codex Alimentarius definitions and recommendations for gluten-free foods. The information in this document can be used as the basis of a harmonized validation protocol for any ELISA method for gluten, whether proprietary or nonproprietary, that will be submitted to AOAC andlor regulatory authorities or other bodies for status recognition. Future work is planned for the implementation of this guidance document for the validation of gluten methods and the creation of gluten reference materials. PMID:24282943

  9. Evaluation of a dot-ELISA for the serodiagnosis of human hydatid diseases

    International Nuclear Information System (INIS)

    A dot-ELISA utilizing antigen-B from sheep hydatid fluid, doted onto nitro nitrocellulose filter discs was developed for the rapid diagnosis of human hydatidosis. 1 micro g of antigen per dot, serum dilution of 1:800, dilution conjugate of 1:1000 and 45 min incubation were found optimal. Thirty four patients infected with hydatidosis, 32 cases with other parasitic diseases and 36 healthy subjects were included in the assay and were examined using dot-ELISA to detect antibody against the aforementioned parasite antigen. Sensitivity, specificity and positive and negative predictive values of the assay were calculated as 97.1% and 98.5% respectively. No false positive reaction was observed when 36 sera healthy subjects were assayed. One case of cross-reactions was observed as for a serum infected with fasciolosis. It was concluded that dot-ELISA is rapid, antigen and serum conservative as well as encompasses great importance to confirm clinical diagnosis either in the laboratory or in the filed. (author)

  10. Biotyping of Clostridium Perfringens strains isolated from enterotoxemia cases in sheep using ELISA technique

    Directory of Open Access Journals (Sweden)

    M. A. Hamad

    2010-01-01

    Full Text Available The study was designed to determine the types of Clostridium perfringens and their toxins in sheep with suspected enterotoxemia in Hama province, Syria. For this purpose, 89 bacterial isolates were isolated from 132 samples collected from dead and some slaughtered sheep with suspected enterotoxemia and diagnosed with classical diagnostic tests and by using enzyme-linked immunosorbent assay (ELISA technique to determine the types and toxins of C. perfringens. These isolates appeared as G+ bulged rods with curved ends and were as positive to lecithinase, gelatin hydrolysis and sugar fermented, as where negative to catalase, oxidase, and indole. Based on the ELISA results all isolates were C. perfringens types A 84 isolate (94.38 %, D 3 (3.37 %, and C 2(2.25 %. Clostridium perfringens type A was the dominant type in cases of enterotoxemia in sheep in Hama province detected by ELISA test. The enterotoxaemia causes considerable economic loss to the sheep industry particularly in Hama province and generally in Syria. Therefore, it is recommended that a proper vaccination schedule against enterotoxemia should be implemented for sheep flocks in Hama province. These vaccines should provide adequate protective immunity against all C. perfringens types specially types A and D.

  11. Outstanding insecurities concerning the use of an Ov16-based ELISA in the Amazonia onchocerciasis focus.

    Science.gov (United States)

    Luz, Sérgio Luiz Bessa; Crainey, James Lee; Shelley, Anthony John; Rubio, Miguel

    2014-07-01

    In a recent issue of Memórias do Instituto Oswaldo Cruz, published in Rio de Janeiro in February 2014 (109: 87-92), Adami et al. have published a survey reporting Mansonella parasite prevalence in the Amazon Region. This report makes a useful contribution to the existing knowledge of filarial parasite distribution within the Amazon area, parasite prevalence rates in relation to age and occupation and provides observations on the possible clinical impact of Mansonella ozzardi. Their publication also provides an account of what appears to be a novel ELISA that has recently been used in the Simuliidae and Onchocerciasis Laboratory of the Oswaldo Cruz Institute, Rio de Janeiro, Brazil. We are concerned that the publication of this ELISA may have created an excessively positive impression of the effectiveness of the onchocerciasis recrudescence serological surveillance tools that are presently available for use in the Amazonia onchocerciasis focus. In this letter we have, thus, sought to highlight some of the limitations of this ELISA and suggest how continuing insecurities concerning the detection of antibodies to Onchocerca volvulus within the Amazonia onchocerciasis focus might be minimised. PMID:25075790

  12. Outstanding insecurities concerning the use of an Ov16-based ELISA in the Amazonia onchocerciasis focus

    Directory of Open Access Journals (Sweden)

    Sérgio Luiz Bessa Luz

    2014-07-01

    Full Text Available In a recent issue of Memórias do Instituto Oswaldo Cruz, published in Rio de Janeiro in February 2014 (109: 87-92, Adami et al. have published a survey reporting Mansonella parasite prevalence in the Amazon Region. This report makes a useful contribution to the existing knowledge of filarial parasite distribution within the Amazon area, parasite prevalence rates in relation to age and occupation and provides observations on the possible clinical impact of Mansonella ozzardi. Their publication also provides an account of what appears to be a novel ELISA that has recently been used in the Simuliidae and Onchocerciasis Laboratory of the Oswaldo Cruz Institute, Rio de Janeiro, Brazil. We are concerned that the publication of this ELISA may have created an excessively positive impression of the effectiveness of the onchocerciasis recrudescence serological surveillance tools that are presently available for use in the Amazonia onchocerciasis focus. In this letter we have, thus, sought to highlight some of the limitations of this ELISA and suggest how continuing insecurities concerning the detection of antibodies to Onchocerca volvulus within the Amazonia onchocerciasis focus might be minimised.

  13. The test of hepatitis C virus antigen by ELISA for clinical diagnosis

    International Nuclear Information System (INIS)

    To provide a testing technique for early clinical diagnosis of HCV infection by detecting HCV antigen(HCAg) through testing serum samples by ELISA, HCV polyclonal antibodies were prepared from animals immunized by mutliepitope complex antigen of HCV expressed by E. coli. Using antibody sandwiched ELISA to detect the HCAg from serum samples, we analyzed the positive rate of HCAg and compared it with the findings of RT-PCR. The positive rate of HCAg was 53.7% from 95 serum samples of the HCAb positive cases; the positive rate of HCAg was 37.5% from 88 serum samples of serious hepatic damage cases, and 11.0% from 73 serum samples of high ALT but with negative HCAb. The results showed that HCAg was related with HCAb(r=0.5076,P<0.01). In 87.5% of the cases, both HCAg and HCV-RNA were positive as compared with RT-PCR. The ELISA method of HCVAg that we developed is a possible technique for early clinical diagnosis of HCV infection. (authors)

  14. ELISA as an alternative tool for epidemiological surveillance for dengue in mosquitoes: a report from Thailand

    Directory of Open Access Journals (Sweden)

    Mayuna Srisuphanunt, Ratana Sithiprasasna, Somboon Patpoparn, Watcharee Attatippaholkun & Viroj Wiwanitkit

    2007-12-01

    Full Text Available Background & objectives: Dengue fever (DF, dengue hemorrhagic fever (DHF and dengue shocksyndrome (DSS are the re-emerging infectious diseases caused by the four serotypes of dengue(DEN virus, type 1 to 4, belonging to the family Flaviviridae and genus Flavivirus. In the absenceof a safe and effective mass immunisation, the prevention and control of dengue outbreaks dependupon the surveillance of cases and mosquito vector. The aim of this work is to test enzyme-linkedimmunosorbent assay (ELISA tool for the virological surveillance of dengue.Methods: Virus-infected Aedes mosquitoes were collected from the field in order to serve as anearly warning monitoring tool for dengue outbreaks. In a prospective field study conducted fromApril to September 2000, female adult Aedes mosquitoes were caught from selected dengue-sensitivearea in Chombung district, Ratchaburi province and assayed by ELISA.Result: Approximately 18.3% were found positive for dengue virus.Conclusion: This can imply that ELISA can be an alternative tool for epidemiological surveillancefor dengue in mosquitoes.

  15. Development and evaluation of the rVP-ELISA for detection of antibodies against porcine parvovirus.

    Science.gov (United States)

    Kong, Miaomiao; Peng, Yonggang; Cui, Yuchao; Chang, Tiecheng; Wang, Xiaoling; Liu, Zhaoxia; Liu, Yonggang; Zhu, Yu; Luo, Yakun; Tang, Qinghai; Feng, Li; Cui, Shangjin

    2014-09-01

    The gene encoding the VP2 protein of porcine parvovirus (PPV) was expressed in an insect-baculovirus system. The recombinant (r) VP2 was similar antigenically/functionally to the native capsid protein as demonstrated by hemagglutination (HA), Western blotting using PPV positive sera. The purified rVP2 proteins were used as coating antigen to establish a rVP-ELISA method for detection of PPV positive and negative sera from pigs. The optimal operating conditions of the rVP-ELISA were: the concentration of rVP2 proteins coated on the wells was 2 μg/mL; the diluted concentration of serum was 1: 150 and that of the enzyme-labeled antibody was 1: 6000. A total of 596 sera were detected by this assay, and the average positive rate was 87%. Compared with France LSI kit, the result showed that the coincidence rate was 96.7%. In conclusion, the rVP2-ELISA is a sensitive and specific method for detecting antibodies against PPV. PMID:24945904

  16. HPLC and ELISA analyses of larval bile acids from Pacific and western brook lampreys

    Science.gov (United States)

    Yun, S.-S.; Scott, A.P.; Bayer, J.M.; Seelye, J.G.; Close, D.A.; Li, W.

    2003-01-01

    Comparative studies were performed on two native lamprey species, Pacific lamprey (Lampetra tridentata) and western brook lamprey (Lampetra richardsoni) from the Pacific coast along with sea lamprey (Petromyzon marinus) from the Great Lakes, to investigate their bile acid production and release. HPLC and ELISA analyses of the gall bladders and liver extract revealed that the major bile acid compound from Pacific and western brook larval lampreys was petromyzonol sulfate (PZS), previously identified as a migratory pheromone in larval sea lamprey. An ELISA for PZS has been developed in a working range of 20pg-10ng per well. The tissue concentrations of PZS in gall bladder were 127.40, 145.86, and 276.96??g/g body mass in sea lamprey, Pacific lamprey, and western brook lamprey, respectively. Releasing rates for PZS in the three species were measured using ELISA to find that western brook and sea lamprey released PZS 20 times higher than Pacific lamprey did. Further studies are required to determine whether PZS is a chemical cue in Pacific and western brook lampreys. ?? 2003 Elsevier Inc. All rights reserved.

  17. Development of an ELISA for evaluation of swab recovery efficiencies of bovine serum albumin.

    Directory of Open Access Journals (Sweden)

    Nadja Sparding

    Full Text Available After a potential biological incident the sampling strategy and sample analysis are crucial for the outcome of the investigation and identification. In this study, we have developed a simple sandwich ELISA based on commercial components to quantify BSA (used as a surrogate for ricin with a detection range of 1.32-80 ng/mL. We used the ELISA to evaluate different protein swabbing procedures (swabbing techniques and after-swabbing treatments for two swab types: a cotton gauze swab and a flocked nylon swab. The optimal swabbing procedure for each swab type was used to obtain recovery efficiencies from different surface materials. The surface recoveries using the optimal swabbing procedure ranged from 0-60% and were significantly higher from nonporous surfaces compared to porous surfaces. In conclusion, this study presents a swabbing procedure evaluation and a simple BSA ELISA based on commercial components, which are easy to perform in a laboratory with basic facilities. The data indicate that different swabbing procedures were optimal for each of the tested swab types, and the particular swab preference depends on the surface material to be swabbed.

  18. Science with the space-based interferometer eLISA. I: Supermassive black hole binaries

    CERN Document Server

    Klein, Antoine; Sesana, Alberto; Petiteau, Antoine; Berti, Emanuele; Babak, Stanislav; Gair, Jonathan; Aoudia, Sofiane; Hinder, Ian; Ohme, Frank; Wardell, Barry

    2016-01-01

    We compare the science capabilities of different eLISA mission designs, including four-link (two-arm) and six-link (three-arm) configurations with different arm lengths, low-frequency noise sensitivities and mission durations. For each of these configurations we consider a few representative massive black hole formation scenarios. These scenarios are chosen to explore two physical mechanisms that greatly affect eLISA rates, namely (i) black hole seeding, and (ii) the delays between the merger of two galaxies and the merger of the black holes hosted by those galaxies. We assess the eLISA parameter estimation accuracy using a Fisher matrix analysis with spin-precessing, inspiral-only waveforms. We quantify the information present in the merger and ringdown by rescaling the inspiral-only Fisher matrix estimates using the signal-to-noise ratio from non-precessing inspiral-merger-ringdown phenomenological waveforms, and from a reduced set of precessing numerical relativity/post-Newtonian hybrid waveforms. We find ...

  19. Use of Competition ELISA for Monitoring of West Nile Virus Infections in Horses in Germany

    Directory of Open Access Journals (Sweden)

    Martin H. Groschup

    2013-07-01

    Full Text Available West Nile virus (WNV is a mosquito-borne viral pathogen of global importance and is considered to be the most widespread flavivirus in the World. Horses, as dead-end hosts, can be infected by bridge mosquito vectors and undergo either subclinical infections or develop severe neurological diseases. The aim of this study was to detect WNV specific antibodies in horses in Germany as an indicator for an endemic circulation of WNV. Sera from more than 5,000 horses (primarily fallen stock animals were collected in eight different federal states of Germany from 2010 to 2012. Sera were screened by a competitive ELISA and positive reactions were verified by an indirect IgM ELISA and/or by virus neutralization tests (VNT for WNV and Tick-borne encephalitis virus (TBEV in order to exclude cross-reacting antibody reactions. In essence WNV specific antibodies could not be detected in any of the horse sera. Not surprisingly, a small number of sera contained antibodies against TBEV. It is noteworthy that equine sera were often collected from horse carcasses and therefore were of poor quality. Nonetheless, these sera were still suitable for WNV ELISA testing, i.e., they did not produce a high background reaction which is a frequently observed phenomenon. According to these data there is no evidence for indigenous WNV infections in horses in Germany at present.

  20. Development of an ELISA kit using monoclonal antibody to Clostridium difficile toxin A

    Institute of Scientific and Technical Information of China (English)

    Si-Wu Fu; Ya-Li Zhang; Dian-Yuan Zhou

    2004-01-01

    AIM: To establish an ELISA kit using monoclonal antibodiesagainst Clostridium difficile ( C. difficile) toxin A.METHODS: An indirect sandwich ElISA was described using the purified rabbit monospecific antiserum as capturing antibody. After the polystyrene microtitre plates with 96 fiat-bottomed wells were coated with rabbit antiserum, the wells were blocked with 100 g/L BSA in PBS-T. C. difficiletoxin A or culture filtrates were added to each well and then monoclonal antibodies IgG-horseradish peroxidase conjugate was added as detecting antibody, tetramethylbenzidine was used as substrate and A450 of the stopped reacting product was recorded in an automated plate reader. RESULTS: The tested specimens included culture filtrates of 2 strains of toxigenic C. difficile, 2 strains of non-toxigenic C. difficile, 26 strains of E. coli, 2 strains of S. dysenteriae, 1 strain of Bif infantis, 5 strains of V. cholera, 2 strains ofS. typhi, 7 strains of C. botulinum, 1 strain of toxigenic C. sordllii, and 1 strain of C. butyricum. A total of 47 strains of culture filtrates were all negative except for 2 strains of toxigenic C. difficile. The detective limitation of toxin A was 0.1 ng/mL.CONCLUSION: An ELISA kit with high specificity and excellent sensitivity for the rapid detection of C. difficile toxin A was established. It will be a useful tool for diagnostic test of C. difficile toxin A.

  1. Low-Frequency Gravitational-Wave Science with eLISA/ NGO

    Science.gov (United States)

    Amaro-Seoane, Pau; Aoudia, Sofiane; Babak, Stanislav; Binetruy, Pierre; Berti, Emanuele; Bohe, Alejandro; Caprini, Chiara; Colpi, Monica; Cornish, Neil J.; Danzmann, Karsten; Dufaux, Jean-Francois; Gair, Jonathan; Jennrich, Oliver; Jetzer, Philippe; Klein, Antoine; Lang, Ryan N.; Lobo, Alberto; Littenberg, Tyson; McWilliams, Sean T.; Nelemans, Gijs; Petiteau, Antoine; Porter, Edward K.; Schutz, Bernard F.; Stebbins, Robin; Vallisneri, Michele

    2011-01-01

    We review the expected science performance of the New Gravitational-Wave Observatory (NGO, a.k.a. eLISA), a mission under study by the European Space Agency for launch in the early 2020s. eLISA will survey the low-frequency gravitational-wave sky (from 0.1 mHz to 1 Hz), detecting and characterizing a broad variety of systems and events throughout the Universe, including the coalescences of massive black holes brought together by galaxy mergers; the inspirals of stellar-mass black holes and compact stars into central galactic black holes; several millions of ultracompact binaries, both detached and mass transferring, in the Galaxy; and possibly unforeseen sources such as the relic gravitational-wave radiation from the early Universe. eLISA's high signal-to-noise measurements will provide new insight into the structure and history of the Universe, and they will test general relativity in its strong-field dynamical regime.

  2. Gravitational-wave radiation from double compact objects with eLISA in the Galaxy

    CERN Document Server

    Liu, Jinzhong

    2014-01-01

    The phase of in-spiral of double compact objects (DCOs: NS+WD, NS+NS, BH+NS, and BH+BH binaries) in the disk field population of the Galaxy provides a potential source in the frequency range from $10^{-4}$ to 0.1 Hz, which can be detected by the European New Gravitational Observatory (NGO: eLISA is derived from the previous LISA proposal) project. In this frequency range, much stronger gravitational wave (GW) radiation can be obtained from DCO sources because they possess more mass than other compact binaries (e.g., close double white dwarfs). In this study, we aim to calculate the gravitational wave signals from the resolvable DCO sources in the Galaxy using a binary population synthesis approach, and to carry out physical properties of these binaries using Monte Carlo simulations. Combining the sensitivity curve of the eLISA detector and a confusion-limited noise floor of close double white dwarfs, we find that only a handful of DCO sources can be detected by the eLISA detector. The detectable number of DCO...

  3. Evaluation of an enzyme linked immunosorbent assay (ELISA) in the serodiagnosis of swine trypanosomosis

    International Nuclear Information System (INIS)

    In this study, the T. evansi antigen used in the ELISA was specific and sensitive for the detection of antibodies against T. evansi in pigs, the antibody being detected in an experimentally infected pig on day 17 post-infection and until the end of the study. In pigs naturally infected with T. evansi, the assay yielded also positive results with high OD values. In uninfected pigs from countries free from trypanosomosis, it only gave a low post positive value. There were no cross-reactions in pigs infected with Toxoplasma and Eperythrozoon. Even though peroxidase recombinant protein G conjugate, instead of specific anti-IgC swine conjugate, was used, it was still effective in ELISA. The antigen was characterized by western blot, and it was found that the predominant polypeptide band was a 67 kD protein. In Thailand, trypanosomosis is found not only in pigs, but also in cattle, buffalo, horses and dogs. Trypanosomosis is widespread in Thailand and difficult to eliminate, so that Ab-ELISA will be an important tool for the diagnosis and epidemiological study of the disease in different animal species, including pigs

  4. Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis

    Directory of Open Access Journals (Sweden)

    Vidal Mônica Scarpelli Martinelli

    2003-01-01

    Full Text Available Chromoblastomycosis (CBM is a chronic subcutaneous infection caused by several dematiaceous fungi. The most commonly etiological agent found in Brazil is Fonsecaea pedrosoi, which appears as thick walled, brownish colored cells with transverse and longitudinal division in the lesions, called "muriform cells". This disease is found worldwide but countries like Madagascar and Brazil have highest incidence. Diagnosis is made by clinical, direct and histopathologic examination and culture of specimens. Serological tests have been used to identify specific antibodies against Fonsecaea pedrosoi antigens, as well as immunotechniques have been used for CBM serological identification and diagnosis. In the present study double immunodiffusion (DID, counterimmunoelectrophoresis (CIE and immunoenzymatic test (ELISA have been used to evaluate humoral immune response in patients with CBM caused by F. pedrosoi. Metabolic antigen was used for immunoprecipitation tests (DID and CIE while somatic antigen for ELISA. Our results demonstrated 53% sensitivity and 96% specificity for DID, while CIE presented 68% sensitivity and 90.5% specificity. ELISA demonstrated 78% sensibility and 83% specificity. Serological tests can be a useful tool to study different aspects of CBM, such as helping differential diagnosis, when culture of the pathogenic agent is impossible.

  5. Molecular detection of Trypanosoma infection: PCR and PCR-ELISA techniques

    International Nuclear Information System (INIS)

    Fast, clear and easy diagnostic methods allow accurate detection of Trypanosomes in human and animal species enabling cost effective treatment and prevention of outbreaks within an epidemic. Diagnostic methods should preferably be usable in the field. Presently many diagnostic field methods are time consuming and their limit for detection of parasites should be decreased. Molecular methods offer the promise of detecting lower numbers of parasites, faster. One focus of this RCM was to develop fast and easy diagnostic methods detecting lower infection levels. The original ideas involved PCR-ELISA and based on our previous experience, we confirm that PCR-ELISA is indeed a powerful method able to detect minor quantities of target DNA, i.e. very low parasite copy numbers, down to a single parasite. We also point out that the method has several pitfalls and should therefore be applied with great care. During the first research coordination meeting in Belgium, we presented results with RT-PCR-ELISA and the controls we used to make our results reliable. This manuscript summarizes these data. (author)

  6. The use of the antigen ELISA for the monitoring of trypanosomal infections in cattle in Zimbabwe

    International Nuclear Information System (INIS)

    The antigen ELISA (Ag-ELISA) for the detection of trypanosomal was used in the diagnosis of bovine trypanosomiasis in Gokwe District, an area from which tsetse flies had been cleared recently; in Hwange Communal Land, which although free from tsetse flies for 20 years, was threatened by reinvasion; and as part of a national survey of vector-borne diseases to determine the extent and prevalence of trypanosomal infections throughout the country. In Gokwe and Hwange all the animals tested were negative for parasites by Buffy Coat Technique (BCT), while the Ag-ELISA results showed that 42.9% and 32.3% respectively of the animals were infected. In the national survey, trypanosomal antigens were detected in cattle at 46.1% of the locations from which samples were collected, most of them in tsetse free areas. These results could be due to reinvasion or persistence of tsetse flies in very small numbers, residual infections of low pathogenicity, or temporary introduction of flies by transport vehicles. (author). 4 refs, 5 figs, 2 tabs

  7. Determination of the accuracy and optimal cut-off point for ELISA test in diagnosis of human brucellosis in Iran.

    Directory of Open Access Journals (Sweden)

    Mehrdad Hasibi

    2013-10-01

    Full Text Available In endemic area the most challenging problem for brucellosis is to find a reliable diagnostic method. In this case-control study, we investigated the accuracy of ELISA test for diagnosis of human brucellosis and determined the optimal cut-off value for ELISA results in Iran. The laboratory diagnosis of brucellosis was performed by blood isolation of Brucella organism with a BACTEC 9240 system and/or detection of Brucella antibodies by standard agglutination test (titer ≥ 1:160. Serum level of ELISA IgG and ELISA IgM from 56 confirmed cases of brucellosis and 126 controls were compared with each other by Box plot graph and Receiver Operating Characteristic (ROC curve. Box plot graphs showed the high degree of dispersion for IgG and IgM data in patients compared with all controls. We observed partially overlapping for IgM data (not for IgG between cases and controls in graphs. The area under ROC curve for distinguishing between cases and controls was larger for IgG compared to IgM. Based on results of this study, ELISA IgG test was more reliable than ELISA IgM test in diagnosis of human brucellosis in Iran. Using a cut-off of 10 IU/ml and 50 IU/ml had most sensitivity (92.9% and most specificity (100% for ELISA IgG test, respectively.

  8. Comparison of Three ELISA Kits for the Differentiation of Foot-and-mouth Disease Virus-infected from Vaccinated Animals

    Institute of Scientific and Technical Information of China (English)

    Yi-mei CAO; Zeng-jun LU; Zai-xin LIU; Qing-ge XIE

    2007-01-01

    A study was performed to validate 3 FMDV 3ABC-I-ELISA kits developed in China for the differentiation of FMDV infected and vaccinated animals.Sets of sera from naive and vaccinated cattle as well as from cattle that had been infected were tested for antibodies against nonstructural proteins (NSPs) of FMDV by commercial diagnosis kits,Ceditest(R)FMDV-NS (Ceditest(R) kit),UBI(R) FMDV NONSTRUCTURAL PROTEIN ELISA DIRECTION INSERT (UBI(R) kit) and a FMDV 3ABC-I-ELISA kitdeveloped at the Lanzhou Veterinary Research Institute.The test parameters (sensitivity and specificity) of the three kits were determined,and the result obtained from FMD 3ABC-I-ELISA kit was compared with that obtained from two foreign kits.The results indicated that the coincidence rate between the FMDV 3ABC-I-ELISA and Ceditest(R) kits was 98.05%,and the coincidence rate between the FMDV 3ABC-I-ELISA and UBI(R) kits was 94.4%; the sensitivity of both Ceditest(R) and FMDV 3ABC-I-ELISA kit was 100%.However,the sensitivity of the UBI(R) kit was only 81.8%.With sera from naive or vaccinated non-infected animals,the specificity of all tests exceeded 90%.

  9. Immune response to Mycoplasma pneumoniae P1 and P116 in patients with atypical pneumonia analyzed by ELISA

    Directory of Open Access Journals (Sweden)

    Birkelund Svend

    2004-02-01

    Full Text Available Abstract Background Serology is often used for the diagnosis of Mycoplasma pneumoniae. It is important to identify specific antigens that can distinguish between the presence or absence of antibodies against M. pneumoniae. The two proteins, P116 and P1, are found to be immunogenic. By using these in ELISA it is possible to identify an immune response against M. pneumoniae in serum samples. Results A recombinant protein derived from the P116 protein and one from the P1 protein were used in two ELISA tests, rP116-ELISA and rP1-ELISA. Human serum samples from patients with atypical pneumonia were tested and compared to the results of the complement fixation test. There was a good agreement between the two tests but the rP1-ELISA showed the best discrimination between positive and negative samples. Conclusion Two ELISA tests based on recombinant proteins have been analysed and compared to the complement fixation test results. The two ELISA tests were found suitable for use in serodiagnostics of M. pneumoniae infections. The use of specific antigens eliminates the risk of cross reaction to an immune response against other bacteria.

  10. Validación operacional de un ELISA comercial para Brucella ovis, Chile Operational validation of a commercial ELISA for Brucella ovis, Chile

    Directory of Open Access Journals (Sweden)

    J F Alvarez

    2007-01-01

    Full Text Available La sensibilidad y especificidad diagnóstica se estiman para una población de referencia, estas difieren de la interpretación operacional o de terreno. El objetivo del presente trabajo es validar operacionalmente un ELISA (Enzyme-Linked Immunosorbent Assay comercial para Brucella ovis en la población ovina de Magallanes. Se emplearon 82 sueros de carneros con aislamiento de B. ovis en semen y 421 sueros de animales con cultivo microbiológico de semen negativo e inmunodifusión en agar gel con resultado negativo. Se estableció un punto de corte de 58,15% de densidad óptica, estimado con el método receiver-operating characteristic; se estimó una sensibilidad de 82,9% y una especificidad de 91,4%; la baja sensibilidad obtenida indica la incapacidad del ELISA para detectar un número considerable de positivos, por lo que en erradicación se deberá utilizar una técnica diagnóstica adicional; el bajo valor predictivo positivo fue influenciado en su mayoría por la baja prevalencia. Los parámetros estimados deberán utilizarse en la interpretación de los futuros análisis diagnósticos y se deberá realizar una validación operacional cuando se adopte una nueva técnica diagnóstica, estableciendo los parámetros con los cuales trabajará el laboratorio y de esta forma minimizar los errores de diagnósticoSensitivity and specificity are usually estimated for a reference population, and these differ from the operational interpretation. The aim of this study was to carry out an operational validation of a commercial Enzyme-Linked Immunosorbent Assay (ELISA for Brucella ovis in the sheep population of Magallanes Region. The assay was validated using sera from 82 rams with bacterial isolation and 421 sera from rams with culture-negative semen and not reacting to agar gel immunodiffusion test. The cut-off point was 58.15% optical density, estimated by using the receiver-operating characteristic method; with 82.9% of sensibility and 91.4% of

  11. Influence of clinical and laboratory variables on faecal antigen ELISA results in dogs with canine parvovirus infection.

    Science.gov (United States)

    Proksch, A L; Unterer, S; Speck, S; Truyen, U; Hartmann, K

    2015-06-01

    False negative faecal canine parvovirus (CPV) antigen ELISA results in dogs with CPV infection are common, but the factors that lead to these false negative results are still unknown. The aim of this study was to investigate whether dogs with a false negative faecal CPV antigen ELISA result have milder clinical signs and laboratory changes, a lower faecal virus load, higher faecal and serum CPV antibody titres and a faster recovery than dogs with a positive result. Eighty dogs with CPV infection, confirmed by the presence of clinical signs and a positive faecal CPV polymerase chain reaction (PCR), were assigned to two groups according to their faecal antigen ELISA result. Time until presentation, severity of symptoms, laboratory parameters, faecal virus load, faecal and serum antibody titres, and CPV sequencing data were compared between both groups. In 38/80 dogs that were hospitalised until recovery, the time to recovery, mortality, and the course of the disease were compared between dogs with positive and negative faecal antigen ELISA results. Of the 80 dogs included, 41 (51.3%) had a false negative faecal antigen ELISA result. ELISA-negative dogs had a significantly shorter time until presentation, lower frequency of defaecation, lower faecal virus load, and higher serum antibody concentrations than ELISA-positive dogs. Laboratory changes, CPV shedding, and outcomes were not associated with faecal antigen ELISA results. In conclusion, low faecal CPV load and antibodies binding to CPV antigen in faeces are likely to be important reasons for false negative faecal antigen ELISA results. Dogs with clinical signs of CPV infection should be retested by faecal PCR. PMID:25920770

  12. Diagnosis of peste des petits ruminants infection in small ruminants through in-house developed Indirect ELISA: Practical considerations

    Directory of Open Access Journals (Sweden)

    K. K. Sharma

    2015-04-01

    Full Text Available Aim: The work was conducted to diagnose peste des petits ruminants (PPR outbreak through an in house developed indirect ELISA (thereafter referred as iELISA its comparison with other available diagnostic tests and description of practical considerations in its development, utility and limitations. Materials and Methods: An outbreak resembled to PPR occurred in two different places of southern Gujarat viz. Vapi and Navsari, affecting 622 animals, including both goat (n = 476 and sheep (n = 146. Animals displayed the typical signs of PPR at Vapi; however diarrhea was the inconsistent feature in animals of Navsari. The affection caused morbidity of 100% and mortality were 73.68% (n = 392/532 and 56.67% (n = 51/90 in Vapi and Navsari outbreaks, respectively. Relevant ante mortem and post mortem samples were collected from representative animals. At the outset of the epidemic no kit was available with us, so agar gel immunodiffusion (AGID was carried out and a commercial ELISA (cELISA kit was ordered for making diagnosis through antibody demonstration. Meanwhile, an iELISA was developed in house using PPR vaccine as antigen and protein G conjugated HRPO antibody as detector. Histopathology and results of sandwich ELISA were also used to diagnose PPR virus (PPRV in the outbreak. Results: The iELISA developed had detected PPRV antibodies in 22/24 samples (91.66%. Significant difference was observed in disease sensitivity pattern of two species by Chi-square test. While AGID failed to detect antibodies in any sample. Results were reconfirmed by comparing with commercially available cELISA kit. Conclusion: PPR is an economically important disease and for the rapid diagnosis of PPR the in house developed antibody capture iELISA can be a suitable cost effective alternative.

  13. A 型肉毒毒素 ELISA 鉴别试验方法的建立%Establishment of an ELISA identification for botulinum toxin type A

    Institute of Scientific and Technical Information of China (English)

    萧在澜; 王媛; 顾磊; 杨雨生; 赵建荣; 徐永浩; 张华捷

    2015-01-01

    Objective To establish a new identification method for botulinum toxin type A ( botA) to replace the traditional animal test.Methods A double antibody sandwich ELISA was established based on the modern immunological technolo-gy, by using prepared two kinds of anti-botA polyclonal antibodies derived from equine and rabbit origin, and a preliminary verification test was performed.Results The established identification ELISA could be a potential alternative method in re-placement of animal test, it is of good specificity, sensitivity, precision and robustness.Conclusion The established method will be expected to be approved for the identification test of botulinum toxin type A as the replacement method for traditional animal test by a further verification and validation.%目的:建立鉴定A型肉毒毒素的ELISA鉴别试验方法以替代传统的动物试验法。方法采用现代免疫学技术,制备马源性和兔源性抗A型肉毒毒素多克隆抗体,建立了双抗体夹心ELISA,并就此初步进行方法学验证。结果所建立的ELISA具有良好的特异性、灵敏度、精密度和耐用性,具有替代动物试验方法的良好前景。结论在进一步验证和确认之后,该方法有望正式成为可用于鉴定A型肉毒毒素的试验方法以替代动物试验法。

  14. Elisa for the diagnosis and epidemiology of Brucella abortus infection in cattle in Chile

    International Nuclear Information System (INIS)

    A serum bank of 1251 adult cows sera was prepared. The sera originated from animals of three different epidemiological groups: 1) 244 from infected cows, strain 19 vaccinated when calves; 2) 507 from herds free of infection but all cows were strain 19 vaccinated when calves and 3) the last group, 500 sera from cows free of infection and non-vaccinated. All the sera where tested with the routine Rose Bengal (RB) Rivanol (RIV) and Complement Fixation (CF) tests and additionally three enzyme immunoassays were performed. They included two indirect Elisa both using the kit from the Joint FAO/IAEA Division, Vienna, Austria. One assay used a polyclonal conjugated antibody (I-ELISAp) and the other a monoclonal conjugated antibody (I-ELISAm). The third assay was a competitive ELISA (C-ELISA) performed with sLPS, plus monoclonal antibody, M84, and goat anti-mouse antibody-HRPO. Using the CFT as 'gold standard' the sensitivities of all the methods were: RB 87.1%, RIV 87.1%, I-ELISAp 100% I-ELISAm 100%. The calculated specificity was: RB 100%, RIV 100%, I-ELISAp 96.4% and I-ELISAm 100%. In the group of infected animals (244) the following results were obtained: RB 13.5%, RIV 11.9%, CF 12.7%, I-ELISAp 50.8% and I-ELISAm 22.9%. Results for the non-vaccinated group were: RB 0.2%, RIV 0%, CFT 0.2%, I-ELISAp 6.9% and I-ELISAm 2.9%. The C-ELISA was performed on samples from the positive group or with positivity values close to the cut-off value in the I-ELISAm. In the infected group 28 out of 63 animals were detected as infected and from the non-vaccinated herds none of 15 I-ELISAm positive samples were detected as infected in the C-ELISA. (author)

  15. Doing Science with eLISA: Astrophysics and Cosmology in the Millihertz Regime

    Science.gov (United States)

    Amaro, Seoane, Pau; Aoudia, Sofiane; Babak, Stanislav; Binetruy, Pierre; Berti, Amanuele; Bohe, Alejandro; Caprini, Chiara; Colpi, Monica; Cornish, Neil J.; Danzmann, Karsten; Dufaux, Jean-Francois; Gair, Jonathan; Jennrich, Oliver; Jetzer, Philippe; Klein, Antoine; Lang, Ryan N.; Lobo, Albsrto; Littenberg, Tyson; McWilliams, Sean T.; Nelemans, Gijs; Petiteau, Antoine; Porter, Edward K.; Schutz, Bernard F.; Stebbins, Robin; Vallisneri, Michele

    2012-01-01

    This document introduces the exciting and fundamentally new science and astronomy that the European New Gravitational Wave Observatory (NGO) mission (derived from the previous LISA proposal) will deliver. The mission (which we will refer to by its informal name eLISA ) will survey for the first time the low-frequency gravitational wave band (about 0.1 mHz to 1 Hz), with sufficient sensitivity to detect interesting individual astrophysical sources out to z = 15. The measurements described here will address the basic scientific goals that have been captured in ESA s New Gravitational Wave Observatory Science Requirements Document ; they are presented here so that the wider scientific community can have access to them. The eLISA mission will discover and study a variety of cosmic events and systems with high sensitivity: coalescences of massive black holes binaries, brought together by galaxy mergers; mergers of earlier, less-massive black holes during the epoch of hierarchical galaxy and black-hole growth; stellar-mass black holes and compact stars in orbits just skimming the horizons of massive black holes in galactic nuclei of the present era; extremely compact white dwarf binaries in our Galaxy, a rich source of information about binary evolution and about future Type Ia supernovae; and possibly most interesting of all, the uncertain and unpredicted sources, for example relics of inflation and of the symmetry-breaking epoch directly after the Big Bang. eLISA s measurements will allow detailed studies of these signals with high signal-to-noise ratio, addressing most of the key scientific questions raised by ESA s Cosmic Vision programme in the areas of astrophysics and cosmology. They will also provide stringent tests of general relativity in the strong-field dynamical regime, which cannot be probed in any other way. This document not only describes the science but also gives an overview on the mission design and orbits. LISA s heritage in the eLISA design will be

  16. SEROEPIDEMIOLOGIC ASSESSMENT OF MALARIA USING AN ELISA FOR RING-INFECTED ERYTHROCYTE SURFACE ANTIGEN OF PLASMODIUM FALCIPARUM

    Directory of Open Access Journals (Sweden)

    J. Kevin Baird

    2012-09-01

    Full Text Available Zat anti terhadap antigen permukaan dari sel eritrosit yang terinfeksi dengan parasit bentuk cincin (Ring-infected Erythrocyte Surface Antigen/RESA Plasmodium falcipa­rum telah diukur dari sera yang dikumpulkan di Jawa Tengah, Jawa Timur, dan Irian Ja­ya. Untuk mendeteksi IgG terhadap RESA tersebut digunakan ELISA (Enzyme Linked Immuno Sorbent Assay. Dari pengukuran tersebut menunjukkan adanya korelasi antara RESA ELISA dengan pengukuran epidemiologis transmisi malaria secara konvensional. Pada tulisan ini diuraikan tentang prosedur standar untuk melakukan RESA ELISA dan membahas hal-hal yang menguntungkan maupun keterbatasan-keterbatasan dalam ekstrapolasi data untuk menggambarkan perkiraan transmisi malaria.

  17. Detection of HBsAg and Anti HBc on donors of a blood bank by IRMA and ELISA methods

    International Nuclear Information System (INIS)

    Comparative evaluation of two methods, Immunoradiometric Assay (IRMA) and Enzyme Immunoassay (ELISA), for detecting HBsAg and Anti HBc was made for determining which is the most advantageous and reliable. The study was made on 300 donors of the Hospital San Juan de Dios Blood Bank. In comparison with the reference method (IRMA), ELISA shows 91.67% of sensitivity. The Anti HBc detection by IRMA is more reliable than the HBsAg detection by IRMA and ELISA for determining the carrier state

  18. Tratamiento de las fracturas diafisarias de húmero mediante osteosíntesis con placa

    OpenAIRE

    Zamora Rodríguez, J. M.; Modrego Aranda, Francisco Javier; Seral García, Belén; Seral Iñigo, Fernando

    2002-01-01

    Hemos tratado 22 facturas agudas diafisarias de húmero mediante reducción abierta y fijación con placa AO entre 1991 y 1999. Todas las fracturas excepto una consolidaron en un plazo medio de 94 días. De acuerdo con el criterio de Brumback los resultados fueron excelentes o buenos en el 90% de los casos. La complicación postoperativa más importante fue la parálisis del nervio radial en 3 casos; todos se recuperaron espontáneamente en un plazo medio de 108 días. La fijación interna mediante pla...

  19. Reconocimiento de textos antiguos mediante técnicas de visión artficial

    OpenAIRE

    Rodríguez Vallejo, Moisés

    2013-01-01

    El objetivo de este proyecto es reconocer caracteres de textos antiguos mediante t ecnicas de visi on arti cial y redes neuronales. Distinguimos dos fases en el proceso: extracci on de los caracteres de las im agenes de los textos antiguos; y el reconocimiento mediante redes neuronales. En la primera utilizaremos las librer as de OpenCV para conseguir el objetivo, mientras que en la segunda fase partimos de un c odigo de una red neuronal de 3 capas de la Universidad Carnegie M...

  20. Simulación y llenado de moldes mediante Moldflow®

    OpenAIRE

    Peidró Roy, Alejandro

    2015-01-01

    El objetivo del presente proyecto es recopilar los conocimientos necesarios para que los alumnos de Formación Professional del ciclo formativo de Diseño mecánico en el módulo M5 Moldes poliméricos, partiendo de cero, puedan diseñar un pieza de plástico y su molde de inyección asociado mediante el software Catia® en los módulos de Part desing, Generative Shape desing y Mold Tooling desing, simularla mediante Moldflow® Plastics, rediseñarla y optimizarla, de forma que el documento que se redact...