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Sample records for cadena mediante elisa

  1. Identificación molecular de Escherichia coli enterotoxigénica en niños con infecciones diarreicas agudas mediante la Reacción en Cadena de la Polimerasa

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    Marx Peña-Hidalgo

    2015-01-01

    Full Text Available Las infecciones diarreicas agudas (IDAs son problemas comunes en niños menores de 5 años en nuestro país y la Región Loreto. Sin embargo, se desconocen los agentes microbiológicos causales de estas infecciones. Para cubrir estos vacíos en el conocimiento, el objetivo de esta investigación fue realizar la identificación molecular de Escherichia coli enterotoxigénica (ECET en niños con infecciones diarreicas agudas mediante la Reacción en Cadena de la Polimerasa (PCR. Se colectaron 188 muestras diarreicas de niños menores de cinco años de siete Centros de Salud de Iquitos. A partir de estas se aislaron cepas de E. coli, se extrajo el ADN y amplificó regiones de los genes que codifican las enterotoxinas lábil al calor (LT y estable al calor (ST. De los 188 casos con IDAs se ha encontrado que ~83% son atribuibles a infecciones por cepas de E. coli y de estas ~18% fueron identificadas molecularmente como ECET por presentar el gen LT (19 o el gen ST (9. En conclusión, la mayoría de las IDAs en niños < 5 años de Iquitos están asociadas con cepas de E. coli. Una fracción importante de estas cepas han sido identificadas molecularmente (mediante PCR y demostrado que pertenecen al patotipo ECET. Asimismo, se ha evidenciado que las cepas ECET portadoras del gen LT predominan con respecto a las que tienen el gen ST y no se ha encontrado cepas ECET que porten de forma simultánea ambos genes.

  2. Determinación de Glifosato mediante inmunoensayo enzimático (ELISA en el Paisaje Protegido Laguna de Rocha y su entorno, Uruguay.

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    Daniela Nardo

    2015-12-01

    Full Text Available En el entorno de la Laguna de Rocha se ha visto incrementada la superficie dedicada a las actividades agrícolas con un mayor uso de plaguicidas, entre ellos el herbicida glifosato, usado en cultivos de soja principalmente. Mediante la utilización de técnicas de inmunoensayo enzimático (ELISA, se investigó la presencia de glifosato en la Laguna y algunos de sus afluentes en dos momentos específicos de tiempo. Se detectó glifosato en 27 de las 28 muestras estudiadas. Muestras positivas por ELISA fueron confirmadas por cromatografía iónica. El método ELISA demostró ser una herramienta de screening adecuada para determinar la presencia de glifosato en agua.

  3. Cambios en el patrón de desarrollo floral de Neoregelia 'Flandria' (Bromeliaceae analizados mediante Cadenas de Markov Floral Developmental Pattern Changes on Neoregelia‘Flandria’(BROMELIACEAE Analysed by Markov Chain methodologyADRIANA

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    Chaparo de Barrera Ángela

    2003-06-01

    Full Text Available Neoregelia 'Flandria'y N. 'Van Durme'son cultivares ornamentales de bromelias. Sussemillas no son viables y la propagación in vitrocausa alteraciones en el variegado delfollaje, por tanto la brotación vegetativa se constituye en la única opción de propa-gación estable. Este artículo hace referencia a los patrones de desarrollo floral (p de72 bromelias y su relación con la brotación vegetativa por efecto de 3 tratamientos:ANA 190 ppm (T1, Ethrel: ANA + ETHREL (T2 y ETHREL, (T3, y divididos poredad: E1 y E2. El resultado de la aplicación de estos tratamientos fue que se obtu-vieron plantas más elongadas y 6 nuevos patrones. Mediante la metodología de cade-nas de Markov se estimaron la evolución de cada patrón hacia los demás a nivel deprobabilidad (si/no y el porcentaje de esta para cada caso.Neoregelia 'Flandria'and N. 'Van Durme'are ornamental cultivars of Bromelia.Propagation by seeds is not viable and prunes constitutes the only way to propagateavoiding alterations. In this article the developmental floral pattern of 72 Bromeliastreated with ANA 190 ppm (T1, Ethrel: ANA + ETHREL,(T2 y ETHREL, (T3 anddivided into two age groups: E1 y E2 are shown. The treatments studied generated moreelongated plants and six new patterns. Using the Markov chain methodology theprobability to evolve to any pattern and the percentage of each were studied.

  4. Gestión de cadenas productivas

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    José María Mendoza Guerra

    2009-01-01

    Full Text Available Resumen:El estudio de las cadenas productivas se enmarca en el análisis colectivo de la competitividad que se derivó deltratamiento de la misma en un territorio específico, en el cual ha sido más popular el estudio de los cluster. Porterintrodujo en su libro Competitive advantage (Porter, 1985 el concepto de cadena de valor, que era un modelo basadoen las actividades de la empresa, la cuales fueron clasificadas por él en básicas y de apoyo. Allí mismo, esteautor reconoció que las empresas pueden enlazarse mediante sus cadenas de valor. Y precisamente este aportees fundamental para concebir una cadena productiva como el conjunto de cadenas de valor de las empresas integrantes,donde dominan las relaciones verticales. Esto último sirve precisamente para diferenciar una cadenaproductiva de un cluster, el cual incluye relaciones verticales y horizontales con una alta complejidad por el mayornúmero de empresas, y en consecuencia, el mayor número de relaciones. La cadena, en cambio, es más simple,porque se fundamenta en las relaciones verticales. Es más, un cluster no es sino un conjunto de cadenas de producciónintegrado alrededor de una industria, en un territorio determinado. Existen dos formas de abordar el estudiode una cadena productiva, el primero parte de una óptica económica de estudio sectorial, así que se enfatizanlas estadísticas de la industria donde se ubica la cadena, por ejemplo, si se trabaja un eslabonamiento alimentariose pueden examinar estadísticas de empleo, contribución al PIB, valor agregado, desempeño en comercio exteriory así sucesivamente. La otra forma de estudiar la cadena está más relacionada con el desempeño empresarialcolectivo de ella, centrado en los eslabones y la operación en general, es decir, la gestión de la cadena. Este últimoenfoque es precisamente el que utilizaré en este artículo.Palabras claves:Gestión, Cadenas productivas, Valor agregado, La gestión de la cadena, Cluster

  5. Contra la cadena perpetua

    OpenAIRE

    Arroyo Zapatero, Luis

    2016-01-01

    La incorporación al Código penal de la cadena perpetua en la reforma de 2015 ha recibido un rechazo casi unánime por parte de la ciencia penal española, que la censura por su frontal oposición a nuestra Carta Magna al vulnerar la prohibición de penas inhumanas y degradantes (art. 15), los principios de culpabilidad y proporcionalidad, el derecho a la libertad (art. 17), el mandato de determinación de las penas (art. 25.1) y el de resocialización (art. 25.2).Este libro es fruto del debate doct...

  6. Evaluación de la longitud de los telómeros en caballos, mediante el uso de la reacción en cadena de la polimerasa cuantitativa

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    L. Sanmartín-Sánchez

    2014-06-01

    Full Text Available Introducción: Los telómeros son estructuras de los cromosomas eucariotas, que juegan un importante papel en el mantenimiento de la estabilidad e integridad celular, estableciéndose como uno de los posibles biomarcadores del envejecimiento celular en el hombre y otras especies. Objetivos: Evaluar la posibilidad del uso de la técnica de la reacción en cadena de la polimerasa cuantitativa (qPCR para calcular la longitud relativa de los telómeros en caballos y asociarla con factores como la edad, la raza y el y el sexo. Material y métodos: Se amplificó una secuencia repetitiva correspondiente a los telómeros, junto con otra de un gen de copia única (Interleukina-2, en muestras de sangre de 175 caballos de tres razas, de distintos grupos de edad y de ambos sexos. Se calculó la longitud relativa de los telómeros de cada individuo a través dos variables de respuesta: la diferencia entre el número de ciclos de cuantificación entre telómeros e Interleukina-2 (Dif Cq y la ratio entre ambos valores (T/S ratio. Resultados: La variable Dif Cq presenta una asociación más fuerte con la edad que la variable T/S ratio. Se observó una correlación negativa entre la edad y la longitud de los telómeros (R= -0,31, p<0,05. Conclusión: La técnica de qPCR es aplicable para determinar la longitud relativa de los telómeros en caballos. Los individuos mayores de 20 años presentan telómeros acortados, aunque no se encontró un efecto significativo ni de la raza, ni del sexo.

  7. Detección de antígenos de la larva de Taenia solium mediante la Técnica de Elisa

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    Carmenza Murillo

    2010-07-01

    Full Text Available Se describe la optimización de las variables en un ELISA sandwich asimétrico para la detección de antígenos circulantes de larva de Taenia solium empleado como estándar líquido intraquístico obtenido del parásito y como suero itunune anticisticerco absorbido con líquido cefalorraquídeo (LCR normal lo que permite detectar 50 ng/ mi de antígeno. Se utilizaron muestras de LCR liofilizadas; bajo estas condiciones de almacenamiento la especificidad obtenida fue del 63% al analizar 24 LCR negativos para neurocisticercosis por tromografía computarizada cerebral, y 15 LCR de pacientes que mostraban calcificaciones. El coeficiente de variación interensayo está dentro de los límites aceptados para el ELISA.

  8. Detección de antígenos de la larva de taenia solium mediante la técnica de elisa

    OpenAIRE

    Carmenza Murillo; Claudia Díaz; Pablo Lorenzana; Santiago Nicholls; Augusto Corredor

    2010-01-01

    Se describe la optimización de las variables en un ELISA sandwich asimétrico para la detección de antígenos circulantes de larva de Taenia solium empleado como estándar líquido intraquístico obtenido del parásito y como suero itunune anticisticerco absorbido con líquido cefalorraquídeo (LCR) normal lo que permite detectar 50 ng/ mi de antígeno. Se utilizaron muestras de LCR liofilizadas; bajo estas condiciones de almacenamiento la especificidad obtenida fue del 63% al analizar 24 LCR ne...

  9. Gobernabilidad y ascenso en la cadena de valor: discusión conceptual

    OpenAIRE

    Seyka Verónica Sandoval Cabrera

    2012-01-01

    La transformación de la base correspondiente al proceso de acumulación expresada mediante el paradigma informático-neoliberal, conduce a la reformulación en cuanto a las estrategias de desarrollo de países y empresas. En el marco de las cadenas de valor global, la estrategia sobre el desarrollo, considera el incremento de la participación en el flujo de valor, respecto a las diferentes rentas producidas en la cadena como centro. La revalorización, el ascenso o upgrading depende, en primera in...

  10. Riesgo de exposición a Fasciola hepática en ganado vacuno en extensivo de Uruguay y Portugal determinado mediante ELISA y un antígeno recombinante

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    J Sanchís

    2015-01-01

    Full Text Available Para evaluar el riesgo de exposición a Fasciola hepatica del ganado vacuno en extensivo se tomaron muestras de sangre de dos zonas climáticas diferentes, Uruguay (clima subtropical húmedo, 1.192 muestras y Portugal (clima mediterráneo, 473 muestras, que se analizaron con un ELISA y un antígeno recombinante de superficie de F. hepatica (FhrAPS. Los datos obtenidos se analizaron de acuerdo con la raza, edad sexo y aptitud. La seroprevalencia global en Uruguay resultó del 56% (Intervalo de Confianza, IC 95%: 53-59 mediante FhrAPS-ELISA, con porcentajes significativamente más altos en vacas lecheras Holstein de 2-4 años. En Portugal, el porcentaje de sensibilización fue del 47% (IC 95%: 43-52 con los valores más elevados en bovinos resultado de cruces de distintas razas, destinados a la obtención de carne y con edad también comprendida entre 2 y 4 años. Se concluyó que el riesgo de exposición a F. hepatica del ganado vacuno en extensivo es mayor en las zonas con clima subtropical húmedo que en las de clima mediterráneo. Los terneros presentan un alto riesgo de exposición al trematodo hepático con independencia de las condiciones climáticas. Los resultados ponen de manifiesto la necesidad de adoptar medidas frente a las formas del parásito que se encuentran en el medio y combinarlas con programas de desparasitación eficaces.

  11. Detección de proteínas prionicas en cerebros de bovino mediante inmunoensayo enzimático. (Detection of prion proteins from bovine’s brains through a ELISA.

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    Farías R., Gustavo

    2007-03-01

    Full Text Available La Encefalopatía Espongiforme Bovina (EEBpertenece a un grupo de enfermedades neurodegenerativas denominadas Encefalopatías Espongiformes Transmisibles (EETs, dentro de las cuales se destacanademás la Enfermedad de Creutzfeld-Jakob (ECJ con su nueva variante (vECJ en los humanos y el Scrapie en animales. Estas patologías se caracterizan por ser mortales, tener un largo período de incubación, signos neurológicos progresivos y por presentar lesiones vacuolares en la materia gris del sistema nervioso central (SNC. El agente etiológico de estas patologías de denomina “prión” (partícula infecciosa de naturaleza proteica, PrP y consiste en una isoforma infectiva (PrPSc, de una proteína constitutiva celular normal (PrPC que posee una gran resistencia a la proteinasa K. Pese a que en Chile no se han diagnosticado casos de EEB, es importante contar conherramientas diagnósticas de alta especificidad, rápidas y reconocidasinternacionalmente, por lo cual se realizó una inmunodetección de PrP mediante un “kit” de inmunoensayo enzimático (ELISA. Se analizaron 90 muestras de óbex bovinos mayores de 30 meses de edad, provenientesde mataderos de la Región Metropolitana. Inicialmente se obtuvieron 2 muestras sospechosas, las que luego de un nuevo ensayo se sumaron al resto de las que inicialmente fueron diagnosticadas como negativas, obteniendo finalmente un 100% de las muestras estudiadas negativas a EEB.A partir de este estudio se establecieron las pautas para la implementación de este método diagnóstico de la EEB en nuestropaís, lo cual nos permite contar con una de las herramientas diagnósticas más rápida, reconocida y utilizada a nivel internacionalpara la prevención, el control y el reconocimiento de la EEB.The bovine spongiform encephalopathy (BSE belongs toa group ofneurodegenerative disease called transmissible spongiform encephalopaties (TSEs that it includes ti Creutzfeld-Jakob`sDisease (CJD with his new variant (v

  12. Direct ELISA.

    Science.gov (United States)

    Lin, Alice V

    2015-01-01

    First described by Engvall and Perlmann, the enzyme-linked immunosorbent assay (ELISA) is a rapid and sensitive method for detection and quantitation of an antigen using an enzyme-labeled antibody. Besides routine laboratory usage, ELISA has been utilized in medical field and food industry as diagnostic and quality control tools. Traditionally performed in 96-well or 384-well polystyrene plates, the technology has expanded to other platforms with increase in automation. Depending on the antigen epitope and availability of specific antibody, there are variations in ELISA setup. The four basic formats are direct, indirect, sandwich, and competitive ELISAs. Direct ELISA is the simplest format requiring an antigen and an enzyme-conjugated antibody specific to the antigen. This chapter describes the individual steps for detection of a plate-bound antigen using a horseradish peroxidase (HRP)-conjugated antibody and luminol-based enhanced chemiluminescence (ECL) substrate. The methodological approach to optimize the assay by chessboard titration is also provided.

  13. Cadenas productivas. Enfoques y precisiones conceptuales

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    Jairo Guillermo Isaza C.

    2008-07-01

    Full Text Available Este artículo presenta una revisión de literatura internacional sobre el origen y la evolución del concepto de cadena productiva, a partir de las teorías tempranas del desarrollo económico y la planeación estratégica. Igualmente, se presenta una reseña sobre el estudio de las cadenas productivas en Colombia, los sectores evaluados y las metodologías empleadas. El estudio concluye con algunas recomendaciones para futura investigación.

  14. Los cuatro arquetipos de cadenas de suministro

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    Salvatore Cannella; Elena Ciancimino; Framinan, Jose M.; Stephen M. Disney

    2010-01-01

    A lo largo del siglo XX las cadenas de suministro han evolucionado para enfrentarse a los desafíos del mercado globalizado y librarse de ineficiencias congénitas como el efecto látigo. En este artículo se presentan cuatros arquetipos de cadenas de suministro, desde la clásica estructura tradicional hasta el innovador paradigma de la configuración sincronizada. Los cuatros arquetipos se presentan según una analogía con los depósitos de agua. Como conclusión, se pone de manifiesto cómo a pesar ...

  15. Impacto de la tecnología de las cadenas hoteleras extranjeras en Cuba por medio de los contratos de administración

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    Gustavo D’Meza Pérez

    2015-12-01

    Full Text Available El sector hotelero en Cuba se clasifica en cadenas hoteleras nacionales, entidades mixtas y alojamientos en casas particulares. Dentro de ellas, las cadenas hoteleras nacionales son de propiedad pública y gestionan sus hoteles directamente (llamados de marcas propias o mediante contratos de administración con otras cadenas extranjeras. Las empresas mixtas siempre contratan a una entidad extranjera para administrar sus hoteles, mientras que los alojamientos privados son administrados por sus propietarios. El objetivo de este trabajo es evaluar el aporte tecnológico que ha significado la presencia de cadenas administradoras extranjeras para las cadenas hoteleras nacionales. Utilizando el análisis estadístico, los criterios de expertos recogidos en un Delphi, la observación directa y la realización de entrevistas, se ha evaluado la calidad de gestión de los diferentes administradores de hoteles (nacionales y extranjeros y el impacto de la difusión de las técnicas extranjeras en la hotelería de marcas propias. Las conclusiones principales son tres: la tecnología de las cadenas hoteleras extranjeras permanece internalizada, su difusión en los grupos nacionales es escasa y la gestión de estos no alcanza los niveles de calidad y posicionamiento de los extranjeros. Para revertir esta situación, se recomiendan acciones que potencien las marcas nacionales y su gestión.

  16. AGREGANDO VALORES A LAS CADENAS DE VALOR

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    Gregory J. Scott

    2014-01-01

    Full Text Available Este artículo presenta una matriz de factores a tomar en cuenta para priorizar las cadenas de valor agrícolas seleccionadas como foco de las iniciativas público-privadas para impulsar su desarrollo. Dichos factores dan especial importancia a temas de responsabilidad social empresarial. Se ilustra su uso con datos y una revisión de la literatura sobre Perú.

  17. Elisa Valero

    OpenAIRE

    Valero Ramos, Elisa

    2009-01-01

    MENOR O IGUAL QUÉ. Los arquitectos, habitualmente aficionados a la matemática sencilla, a los enteros más que a los quebrados, a los módulos, las series, los ejes, las simetrías y las transparencias, admiramos ese oximorón miesiano de ¿menos es más¿ que es, también, el sumum del expresionismo elemental. Y tal vez la arquitecta Elisa Valero, cuya obra autodenominada menor tengo el honor de glosar con estas letras, milita también en este orden de ideas. Educada a partes iguales entre e...

  18. Riesgo de exposición a Fasciola hepática en ganado vacuno en extensivo de Uruguay y Portugal determinado mediante ELISA y un antígeno recombinante

    OpenAIRE

    Sanchís, J.; GV Hillyer; LM Madeira de Carvalho; MI Macchi; C. Gomes; G Maldini; G. Stilwell; JM Venzal; Paz-Silva, A.; Sánchez-Andrade, R.; MS Arias

    2015-01-01

    Para evaluar el riesgo de exposición a Fasciola hepatica del ganado vacuno en extensivo se tomaron muestras de sangre de dos zonas climáticas diferentes, Uruguay (clima subtropical húmedo, 1.192 muestras) y Portugal (clima mediterráneo, 473 muestras), que se analizaron con un ELISA y un antígeno recombinante de superficie de F. hepatica (FhrAPS). Los datos obtenidos se analizaron de acuerdo con la raza, edad sexo y aptitud. La seroprevalencia global en Uruguay resultó del 56% (Intervalo de Co...

  19. Detección de anticuerpos contra el virus de la peste porcina clásica mediante la prueba inmunoenzimática (CIV-ELISA y seroneutralización Detection of antibodies against the classical swine fever virus by enzime-linked immunosorbent assay (ELISA

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    A. ISLAS

    1997-01-01

    Full Text Available La peste porcina clásica (PPC es una enfermedad de gran importancia mundial. En Chile está sometida a un programa de control y erradi-cación. El presente ensayo tuvo como objetivo estandarizar y comparar una técnica inmunoenzimática (CIV-ELISA que detecta anticuerpos del tipo IgG dirigidos contra el virus de la PPC, con la seroneutralización (SN, técnica oficial para el diagnóstico serológico. Se utilizaron 20 cerdos híbridos Landrace x Large White de 20 kg de peso vivo, los que fueron inoculados experimentalmente con una dosis de 100.000 DICT50 del aislado virulento Quillota del virus PPC. Un grupo constituido por 4 cerdos fue vacunado 14 días antes de la inoculación. Se tomaron muestras de sangre a los 3, 6, 9, 12 y 14 días post-inoculación. Además, se obtuvieron muestras serológicas de 100 hembras reproduc-toras. Los anticuerpos anti-PPC fueron determinados por un CIV-ELISA policlonal, realizándose comparación con la prueba de SN. La prueba CIV-ELISA no detectó anticuerpos en los cerdos inoculados experimentalmente hasta los 14 días post-inoculación. Sin embargo, los cerdos vacunados previamente fueron seropositivos, situación ratificada por la SN. La sensibilidad y especificidad del CIV-ELISA fue de 90.1% y 76.4%, respectivamente. El CIV-ELISA demostró ser una prueba de fácil aplicación y los resultados son similares a los de la SNClassical swine fever (CSF is an important worldwide disease. In Chile, it is subject to a control and erradication programme. The objective of the present assay was to standardize an immunoenzimatic technique (CIV-ELISA that detects antibodies of the IgG type directed against the CSF virus and to compare it with seroneutralization. Twenty hybrid Landrace x Large White pigs with a live weight of 20 kg were used. They were experimentally inoculated with a dose of 100.000 TCID50 of the Quillota virulent isolate of the CSF virus. A four-pig group was vaccinated 14 days before inoculation

  20. Cadenas de valor: un enfoque para las agrocadenas

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    Yadira Peña

    2008-01-01

    Full Text Available Este artículo tiene como propósito mostrar elementos de análisis para abordar el tema de las cadenas de valor. Tras una revisión exhaustiva de bibliografía relacionada y aplicada a la agricultura, se dan a conocer herramientas conceptuales que coadyuvan a construir un escenario claro y conducente para que las cadenas logren mejores niveles de competitividad, crecimiento y desarrollo para su entorno. En la primera y segunda parte se desarrolla el concepto de cadena de valor y se muestran los alcances del enfoque; en la tercera parte se plantean algunas consideraciones sobre la gestión de las cadenas de valor y en la última se ilustran dos casos exitosos en el desarrollo de cadenas de valor.

  1. Diagnóstico de absceso hepático amibiano mediante el inmunoensayo enzimático ligado a una enzima (ELISA Diagnosis of amebic liver abscess by means of an anzyme-linked immunoabsorbent assay

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    Jorge Humberto Botero Garcés

    2001-02-01

    Full Text Available El absceso hepático amibiano (aha es la complicación extraintestinal más frecuente de la amibiasis; su cuadro clínico es generalmente agudo y se lo considera una urgencia médica. Se manifiesta con síntomas generales, acompañado de dolor en hipocondrio derecho, que se puede irradiar al hombro; la hepatomegalia dolorosa es un signo sugestivo pero no diagnóstico; debe diferenciarse del absceso hepático piógeno y del hepatoma necrótico; para el enfoque diagnóstico se requieren ayudas imaginológicas y pruebas de laboratorio como las inmunológicas. En este estudio se puso a punto la prueba de ELISA para la determinación de anticuerpos antiamibianos en sueros controles de pacientes con AHA, estandarizada en el Instituto Nacional de Salud de Bogotá. Además se evaluaron 67 muestras de pacientes con sospecha clínica de AHA, procedentes del Hospital González Valencia de Bucaramanga y del Hospital Universitario San Vicente de Paúl de Medellín. El 70.2% de los sueros problema evaluados fueron positivos, con una tasa de positividad significativamente mayor en los procedentes de Bucaramanga. Amebic liver abscess (ALA is the most frequent extraintestinal complication of amibiasis; its clinical presentation is generally acute, and it is considered a medical emergency. Patients manifest general symptoms, accompanied by pain in the right hypochondrium that can irradiate to the shoulder; painful hepatomegalia is a suggestive but not diagnostic sign; it should be distinguished from pyogenic liver abscess and necrotic hepatoma; for the diagnostic approach to ALA imagenologic exams and laboratory tests are required, among the latter the immunologic ones. In this study we used the ELISA test for the determination of antiamebic antibodies in control ALA sera, as described by the National Institute of Health Bogotá, Colombia (7. Furthermore, 67 serum samples were evaluated from patients with clinic suspicion of ALA. Of these 70.2 were positive

  2. Frequency of human toxocariasis in a rural population from Cajamarca, Peru determined by DOT-ELISA test Freqüência de toxocaríase humana numa população rural de Cajamarca, Peru, mediante o uso do teste DOT-ELISA

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    William H. Roldán

    2009-04-01

    Full Text Available The aim of this study was to estimate the frequency of human toxocariasis in Cauday district, Cajamarca, Peru, using a dot-ELISA test. From June to October 2005, a total of 256 adult subjects were studied. Blood samples were collected for serology by a dot-ELISA test and for hematological examination. Parasitological examination was also carried out in stool samples to check cross-reactions in the dot-ELISA. The frequency observed was 44.92%, with a significant higher proportion of positivity in male subjects. From subjects with positive serology, 45.6% had respiratory symptoms, 40.44% abdominal pain, 32.35% hepatic symptoms, 14.7% cutaneous signs, 13.23% ocular manifestations, 43.38% eosinophilia, and all of these were statistically associated to serology. Among the population evaluated, 90.23% (231/256 were parasitized. From subjects with positive serology, 92.17% had at least one intestinal parasite and the most frequent were: Blastocystis hominis (68.38%, Giardia lamblia (28.68%, Hymenolepis nana (20.0%, Ascaris lumbricoides (15.65%, Entamoeba histolytica/E. dispar (13.24%, Cyclospora cayetanensis (4.41%, Cryptosporidium sp. (1.47%, Enterobius vermicularis (0.87%, Strongyloides stercoralis (0.87%, Taenia sp. (0.87%, and Trichuris trichiura (0.87%. The rate of false positives in the dot-ELISA test was improved by serum absorption each with A. suum antigens, with a decrease of cross-reactions. In conclusion, human toxocariasis is highly frequent in this population and some risk factors like dog/cat ownership, presence of pets within house, and previous history of geophagia were observed in the present study.O propósito do presente estudo foi estimar a freqüência da toxocaríase no distrito de Cauday, Cajamarca, Peru, usando o dot-ELISA teste. Entre junho e outubro de 2005, um total de 256 pessoas foram avaliadas. Coletaram-se amostras de sangue para o teste de dot-ELISA e para o exame hematológico e amostras de fezes para exame parasitol

  3. RESEÑA DEL SOFTWARE DISPONIBLE EN COLOMBIA PARA LA GESTIÓN DE INVENTARIOS EN CADENAS DE ABASTECIMIENTO

    OpenAIRE

    VALENTINA GUTIÉRREZ; DIANA PATRICIA JARAMILLO

    2009-01-01

    La gestión de los diferentes tipos de inventario a lo largo de una cadena de abastecimiento es un problema complejo que, en la mayoría de los casos de la industria colombiana e internacional, se aborda mediante la implementación de herramientas de software. Sin embargo, la decisión de implementar dichas herramientas está en muchos casos limitada por la capacidad financiera para invertir en ellas y por el desconocimiento de la oferta de software disponible en Colombia. Este artículo presenta u...

  4. ENCEFALITIS HERPÉTICA EN PREESCOLAR CONFIRMADO POR REACCIÓN EN CADENA DE LA POLIMERASA

    Directory of Open Access Journals (Sweden)

    Christian M. Chiara Chilet

    2014-01-01

    Full Text Available La Encefalitis Herpética (EH es una enfermedad infecciosa del Sistema Nervioso Central (SNC severa, causado casi exclusivamente por el virus Herpes simple(VHS tipo 1, sin tratamiento existe una mortalidad del 70%. Se describe un preescolar diagnosticado de EH confirmado por Reacción en Cadena de la Polimerasa(RCP en Líquido Cefalorraquídeo (LCR, quien ingresa al Hospital Nacional Guillermo Almenara Irigoyen(HNGAI con un cuadro clínico progresivo de 23 díasde evolución, caracterizado por fiebre (38.5 °C, trastorno de conciencia, malestar general, movimientos involuntarios y rigidez generalizada. El tratamiento instauradofue Aciclovir por 21 días. El propósito de este caso clínico es dar a conocer que el diagnóstico de EH se basa principalmente en su sospecha en todo pacientecon encefalitis y su confirmación diagnóstica mediante RCP en LCR. El cuadro clínico, los resultados de laboratorio y la imagenología son importantes para eldiagnóstico, tratamiento precoz y el pronóstico.Palabras Clave: Herpes Simple, Encefalitis, Reacción en Cadena de la Polimerasa, Preescolar

  5. El estudio de la cadena productiva del fique

    Directory of Open Access Journals (Sweden)

    Maria Eugenia Morales Rubiano

    2002-09-01

    Full Text Available El estudio recopila los resultados más importantes obtenidos en el trabajo de grado denominado "Estudio de factibilidad de la cadena productiva del fique cultivado en Colombia", el cual tomo como punto de referencia el sector fiquero para estimas la factibilidad de aplicar un esquema de cadena productiva, a través de la estructura y análisis estratégico del sistema.

  6. Selección de cadenas para equipos de transporte continuo. // Chain selection software for continuous conveyors.

    Directory of Open Access Journals (Sweden)

    F. Aguilar Parés

    2003-01-01

    Full Text Available El diseño de transportadores y elevadores, que usan cadenas como órgano motor, es algo común en la industria. Unelemento esencial para el buen funcionamiento del equipo es seleccionar correctamente la cadena. Los fabricantes decadenas disponen de una amplia gama de cadenas y aditamentos para satisfacer cualquier requerimiento del equipodiseñado, pero precisamente ante tal universo de cadenas es que surge la necesidad de establecer un procedimiento quecontemple:· Tipo de equipo diseñado (transportador de tablillas o de rastrillos o elevador de cangilones.· Requerimientos de transportación (capacidad, velocidad de transportación, características del material, etc..· Condiciones de transportación (sobrecarga, condiciones de operación, horas de trabajo, etc..El procedimiento antes descrito resulta ser engorroso porque hay etapas en que una decisión implica ajustes a cálculosanteriores y porque como en cualquier proceso de diseño se puede obtener mas de una solución. Por tal motivo se acometióla realización del programa de computación SELCAD. El programa está concebido para que se obtengan resultados porpersonas que no son expertas en el diseño y selección de equipos de transporte continuo, pudiéndose usar también paravalorar si una cadena dada está bien empleada. La base de datos fue obtenida de un catálogo de la Link Belt IndustrialChain Division en que se valoran el uso de 14 tipos de cadenas, con un promedio de 15 modelos por tipo de cadena, lascuales disponen de 40 tipos de aditamentos diferentes. Algunas características del programa son:· Ambiente fácil para el usuario (programado en Visual Basic versión 5.· Sistema de ayudas a lo largo del programa.· Brinda las características del órgano portador y de la rueda motriz.· Permite visualizar, mediante fotos, la forma real de la cadena y del aditamento seleccionado.· Análisis rápido de variantes diferentesPalabras claves Cadenas de transportaci

  7. Análisis de comportamiento caótico en variables de la cadena de suministro

    Directory of Open Access Journals (Sweden)

    Sergio A. Ramírez

    2011-12-01

    Full Text Available Este artículo desarrolla un modelo de cadena de suministro de cuatro niveles mediante la utilización de la dinámica de sistemas con el software Ithink® y Mathlab® para el análisis de los datos. La cadena de suministro se estudia a partir de la Teoría del Caos, teoría que ayuda a identificar cuáles son las variables más sensibles que puedan llevar a un estado de caos. Se construye un modelo de estructura genérica que comprende los eslabones de fábrica, distribución, mayorista, minorista y cliente. Se simula la dificultad que los gerentes de operaciones y logística encuentran para decidir sobre cuánto ordenar y en qué momento, de acuerdo con lo que ocurre en los eslabones del nivel anterior y posterior, mediante dos parámetros de decisión: el primer parámetro denominado A indica la participación de la cantidad de unidades (entre 0 y 1 que se tiene en cuenta de los productos en el inventario actual y los pedidos pendientes, al momento de hacer el pedido. El segundo parámetro denominado B es la participación de la cantidad de unidades (entre 0 y 1 que se encuentran en la línea de suministro (en tránsito al momento de hacer el pedido en cada uno de los eslabones de la cadena de suministros.

  8. INMUNODIAGNOSTICO DE FASCIOLOSIS BOVINA MEDIANTE ELISA Y WESTERN BLOT

    OpenAIRE

    Texia Gorman; Roxana Sanchez; Fernando Fredes; Hector Alcaino

    1998-01-01

    Se evaluó y caracterizó la respuesta inmune humoral de bovinos naturalmente infectados con Fasciola hepatica frente a un extracto total de antígenos de excreción-secreción (E-S) y a una fracción antigénica semipurificada cromatográficamente (

  9. Decisiones en la gerencia de la cadena de suministro

    OpenAIRE

    DRANGO SERNA, MARTÍN D.; PÉREZ ORTEGA, GIOVANNI; ARANGO MARTÍNEZ, CARLOS A.

    2009-01-01

    Las presiones y desafíos de la globalización han llevado a las organizaciones de hoy a ser cada vez más competitivas si quieren sobrevivir en este ambiente. En ese afán de mejorar la competitividad, las organizaciones tratan de alinear sus objetivos e intereses con otras compañías en lo que se conoce como la cadena de suministro. Esa alineación de objetivos no es fácil, surge entonces la gerencia de la cadena de suministro, que pretende la integración de los procesos claves de las organizacio...

  10. Antecedentes de reputación corporativa en cadenas televisivas

    Directory of Open Access Journals (Sweden)

    Natalia Vila-López

    2013-01-01

    Full Text Available Desde el presente trabajo se ha estudiado la reputación corporativa para un ámbito concreto: el de las cadenas televisivas en España. Precisamente en el sector de las empresas de radiodifusión, y más concretamente en el contexto de las cadenas televisivas, el término de reputación corporativa cobra especial relevancia. La proliferación de medios y la continua fragmentación de la audiencia durante las dos últimas décadas han cambiado el escenario del mercado de los medios de comunicación. La mayor competencia está facilitando la aplicación del brand management en muchas industrias de medios, consideradas como firmas en auge por establecer una imagen de marca clara y memorable en el cada vez más complicado mercado lleno de infinitos contenidos. En este ámbito, resulta una prioridad identificar cómo lograr la reputación corporativa de una cadena televisiva desde el punto de vista de la demanda, diferenciando entre medidas dirigidas a gestores de cadenas públicas y privadas.

  11. OPTIMIZACIÓN DE LOS NIVELES DE INVENTARIO EN UNA CADENA DE SUMINISTRO / INVENTORY LEVELS OPTIMIZATION IN A SUPPLY CHAIN

    Directory of Open Access Journals (Sweden)

    José Antonio Díaz-Batista

    2012-04-01

    Full Text Available La gestión de inventarios es un tema muy estudiado por la Gestión de Operaciones y la Investigación de Operaciones para optimizar el costo de la gestión de los inventarios. Los modelos de inventario tradicionales generalmente trataban la optimización de inventarios para una organización individual. Pero recientemente ha comenzado a imponerse en la gestión empresarial el concepto de “cadena de suministro”, donde la satisfacción de las necesidades del cliente final se logra mediante los flujos material, de información y financiero, que se establecen desde los proveedores originales hasta el último consumidor, requiriendo esto de un determinado nivel de cooperación e integración entre los participantes en la cadena. En este trabajo se estudia la optimización de los inventarios conjuntos suministrador-comprador, comparándolos con las políticas tradicionales no colaborativas, analizándose, como conclusión, las ventajas de este enfoque en los costos totales de inventario en la cadena.AbstractInventory management is a strongly observed subject in Operations Management and Operations Research, seeking for inventory management cost optimization. Traditional inventory models mainly deal with optimization inside a single enterprise. Yet, actually the supply chain concept has begun to overcome others approaches in the business management area. Supply chain management seeks customer satisfaction through material, financial and informational flows that are establish throughout the chain, from suppliers until the final consumer. This requires certain level of coordination and integration among supply chain members. This paper studies join supplier-buyer inventory optimization, comparing them with traditional non-collaborative policies, and analyzing advantages of this collaborative approach in the total inventory costs of the supply chain.

  12. Análisis FODA de la cadena arrocera del Estado Portuguesa considerando los procesos de integración con el Mercosur y la CAN

    OpenAIRE

    Vivas Sánchez, Víctor Vicente; Albisu Aguado, Luis Miguel

    2010-01-01

    La cadena arrocera del Estado Portuguesa, posee condiciones especiales para el desarrollo del cultivo y del proceso agroindustrial consiguiente, tales como, elevado potencial de tierras con condiciones para la producción de este cereal, incrementos en producción obtenidos mediante mejoramiento de las técnicas de manejo y fitomejoramiento, productores con un elevado nivel técnico y adecuada infraestructura productiva, así como presencia de un liderazgo real y de organizaciones gremiales sólida...

  13. ELISA u analitici hrane

    OpenAIRE

    Runje, Mislav; Cvrtila, Željka

    2006-01-01

    U radu su prikazane neke od mogućnosti korištenja “screening” testa ELISA u analitici namirnica. Utvrđivanje vrsta mesa u toplinski obrađenim ili, pak, toplinski neobrađenim proizvodima jedna je od primjena ELISA testova.

  14. Cambio de concepto de Compras a Cadena de Suministros

    OpenAIRE

    Rafael Nogales González

    2007-01-01

    Se expone la necesidad de cambiar el concepto de compras a una dimensión mayor e incluida en una gestión más eficiente. Se presenta un marco teórico de las principales funciones del área de aseguramientos así como inicios de la Logística en la gestión empresarial. Se estudian los métodos de aprovisionamiento, las principales funciones del departamento de compras y como éste se integra en la nueva filosofía de la Cadena de Suministros. Se exponen las ideas principales del Sistema de Gestió...

  15. Optimización de una cadena de suministro

    OpenAIRE

    Antúnez Ruiz, Marta

    2006-01-01

    El presente proyecto se ha realizado bajo un convenio de cooperación educativa entre Universidad y Empresa en una planta de productos químicos de una compañía de gran consumo. El proyecto se basa en la optimización de una cadena de suministro a través de la eliminación de las pérdidas existentes, entendiendo como pérdidas todas las operaciones que no dan valor añadido al producto final. La metodología usada es el Value Stream Mapping, una herramienta del Lean Manufacturing. La ...

  16. Cornelius Elisa Bertus Bremekamp

    NARCIS (Netherlands)

    Lanjouw, J.

    1969-01-01

    Cornelis Elisa Bertus Bremekamp was born at Dordrecht on February 7th 1888. He is therefore now just past eighty, and he has been a member of the Koninklijke Nederlandse Botanische Vereniging for sixty years. He studied at the Utrecht State University and, like many of his contemporaries, was strong

  17. Dynex: multiplex ELISA technology

    Science.gov (United States)

    Conventional enzyme linked immunosorbent assay (ELISA) is a gold standard for screening antibodies and testing for protein or antigen presence. A significant limitation of this assay resides in the fact that only one analyte can be assessed per microplate well. Here, we describe and investigate a ne...

  18. Similitud melódica como transformación de cadenas I.

    OpenAIRE

    Gomez Martín, Francisco

    2013-01-01

    He aquı una nueva serie de tres artıculos que investigan desde un punto de vista divulgativo la relacion entre la similitud melódica y la teoría de cadenas (una rama de la computación). La similitud melódica, bajo ciertas hipótesis que simplifican el complejo fenómeno que es, se puede con- cebir como un problema de comparación de cadenas, donde las cadenas aquí representan sucesiones de notas. En un artículo titulado Comparison of musical sequences Mongeau y Sankoff [MS90]explotaron es...

  19. Generando entrelazamiento en cadenas XY - (Generating entanglement in XY chains)

    CERN Document Server

    Schmiegelow, C T

    2006-01-01

    Se estudia en este trabajo la capacidad de generar entrelazamiento de una cadena de espines con acoplamiento de Heisenberg XY y un campo magnetico uniforme a partir de un estado inicial en el que los espines estan completamente alineados. Se encuentra que la capacidad de generar estados entrelazados no muestra un comportamiento monotono con el campo presentando, en cambio, plateaus y resonancias. Tambien se muestra que, a pesar de que la anisotropia es necesaria para que se generen estados entrelazados, una mayor anisotropia no implica necesariamente mejores condiciones para generar entrelazamiento que sirva para usarse en una computadora cuantica. Inclusive, se observa que, se genera una cantidad finita de entrelazamiento en el limite de pequena anisotropia. (The maximum entanglement reached by an initially fully aligned state evolving in a XY Heisenberg spin chain placed in a uniform transverse magnetic field is studied. It is shown that the capacity to create entangled states (both of one qubit with the re...

  20. La cadena de valor del sector confección

    Directory of Open Access Journals (Sweden)

    Maryana Sandrea

    2004-01-01

    Full Text Available El alto grado de competitividad empresarial sumado a los efectos del fenómeno de la globalización, abren nuevos retos y oportunidades para las empresas locales. Dentro de este contexto, el presente artículo tiene como objetivo identificar los componentes cualitativos de la cadena de valor para el producto líder del sector confección en el municipio Maracaibo del estado Zulia (Venezuela; por medio de un estudio de tipo descriptivo-transeccional, utilizando entrevistas semiestructuradas. De acuerdo a los resultados obtenidos las franelas constituyen el producto líder para la mayoría de las empresas entrevistadas. La construcción de la cadena de valor, mostró que la mayor parte de los gerentes del sector (72,73% para 1999 y 66,67% para el 2003 consideraban a la diferenciación del producto como el factor determinante en la decisión de compra del cliente. Al mismo tiempo, un 54,55% en 1999 y un 81,82% en el 2003, identificó a la producción como la etapa clave en la generación de valor. Por lo que, se estiman como fundamentales las actividades primarias de operaciones y logística de entrada, así como las actividades de apoyo relativas al recurso humano, y al desarrollo de tecnologías. Finalmente, el estudio plantea como fuentes potenciales de ventajas competitivas, la optimización de actividades internas y el análisis de los nexos con proveedores, pudiendo aplicarse una estrategia competitiva de diferenciación con minimización del impacto en costos.

  1. ESTADO DEL ARTE EN REDES VANET Y CADENAS DE MARKOV

    Directory of Open Access Journals (Sweden)

    Jorge Blanco

    2012-02-01

    Full Text Available El  presente  artículo  resume  el  estado  del  arte  de  las redes  VANET  como  una  extensión  de  las  redes MANET,  por  tener  características  particulares  en cuanto a la forma de construir topologías que cambian   dinámicamente  en  el  tiempo,  en  tal  sentido,  se proponen los argumento para que en futuros trabajos de  investigación,  se  haga  un  análisis  no  desde  el punto  vista  de  simulación,  sino  desde  el  punto  de vista  matemático  para  que  se  desarrolle  a  través  de cadenas de Markov, ya que la construcción de redes VANET corresponde o puede verse como  un proceso estocástico    discreto,    que    puede   ser   analizado matemáticamente por medio de dichas cadenas,  con el fin de garantizar una  entrega confiable de paquetes de  información  entre  vehículos  los  cuales  están entrando  y  saliendo  constantemente  dentro  de  la topología de red.

  2. Estrategias de nivel simple en cadenas de suministro de repuestos

    Directory of Open Access Journals (Sweden)

    Oswaldo Moscoso Zea

    2013-12-01

    Full Text Available (Recibido: 2013/09/30 - Aceptado: 2013/12/02La Gestión de Inventarios en Cadenas de Suministro de Repuestos (GICSR juega un rol importante tanto para la Compañía Cliente que desea mantenerse operativa a pesar de fallas en la maquinaria o equipos y la Compañía Proveedora o Productora que desea mejorar sus procesos de servicios de post venta. El incremento en la importancia de GICSR requiere la búsqueda de la mejora e innovación de las estrategias en esta área. Este artículo describe estrategias de Nivel Simple de gestión de inventarios en prácticas de administración de repuestos y sirve como una fuente para decidir sobre la mejor estrategia en función del tipo de negocio y la aplicación. Hoy en día estas prácticas no son solo una obligación legal de una organización sino también una oportunidad de construir lealtad y relaciones comerciales a largo plazo con los clientes.

  3. Herramientas computacionales avanzadas para la gestión integrada de la cadena de suministro

    OpenAIRE

    Galli, María Rosa; Chiotti, Omar Juan Alfredo

    2001-01-01

    La gestión integrada de la cadena de suministro no puede sustentarse en la relación jerárquica convencional e implica una nueva forma de relación estratégica basada en un modelo de colaboración entre las empresas. Este modelo de colaboración implica rediseñar los mecanismos y las técnicas de gestión de la cadena de suministro interna de cada empresa, con la visión de dar soporte a los procesos de colaboración y negociación que la misma debe mantener con las otras entidades de la cadena de sum...

  4. La Cadena de Suministro de la Energía Solar

    OpenAIRE

    Virginia Guadalupe López Torres; Carmen Alcalá Álvarez; Luis Ramón Moreno Moreno

    2012-01-01

    Se presenta la cadena de suministro instalada en México para el mercado de la energía solar, con base a una investigación exploratoria realizada en bases de datos especializadas de empresas dedicadas a la fabricación, proveeduría, comercialización y prestación de servicios de sistemas solares. Los resultados indican la no existencia en México de una cadena de suministro articulada, sólo se perciben eslabones aislados, por ello se plantea que el gobierno a través de políticas públicas fomente ...

  5. Empresas y territorio: Cadenas productivas, redes, innovación y competitividad

    OpenAIRE

    Carlo Pietrobelli

    2009-01-01

    Esta presentación ofrece una revisión de los asuntos relacionados con cadenas de valor y la productividad de empresas. El documento explica conceptos como innovación, "upgrading", competición, organización de negocios, oportunidades y rentas. Utilizando un análisis de varias teorías y casos de producción específicos, la presentación indica algunos resultados. Se muestra que la eficiencia económica colectiva ayuda al proceso de upgrading y cómo el modelo de governance de cadenas afecta el upgr...

  6. Innovación tecnológica de la cadena agroalimentaria de maíz para mejorar su competitividad: estudio de caso en el estado de Hidalgo Agroalimentary maize chain technological innovation to improve its competitiviness: case study at Hidalgo State

    Directory of Open Access Journals (Sweden)

    Georgel Moctezuma-López

    Full Text Available El objetivo de esta investigación fue identificar la oferta y demanda tecnológica para plantear alternativas en la innovación de la cadena agroalimentaria de maíz en el estado de Hidalgo, México. Se realizaron las siguientes etapas: 1 caracterización de la cadena; 2 identificación de los factores críticos; 3 distinción de demandas tecnológicas de conocimiento y política; 4 reconocimiento de la oferta tecnológica y 5 cuantificación de proyectos de investigación y política pública. Se aplicó el enfoque de cadenas agroalimentarias y se realizaron dos foros de consulta para captar las demandas de los eslabones de la cadena. Mediante revisión bibliográfica se integró la oferta tecnológica de la cadena en estudio, posteriormente se contrastó con la demanda obtenida para identificar proyectos que impulsen la cadena. Se identificaron 23 demandas: seis de capacitación, tres de validación y transferencia de tecnología, seis de nuevo conocimiento, tres de nueva tecnología y cinco de política pública. Se detectó que existe tecnología y conocimientos para atender 10 de ellas, por lo que existe la necesidad de apoyar 13 proyectos tendientes a mejorar la innovación tecnológica de la cadena maíz en el estado de Hidalgo.The objective of this research was to identify the technological supply and demand and to raise agroalimentary maize value chain alternatives innovation at the state of Hidalgo, Mexico. The following stages were realized: 1 value chain characterization; 2 identification of critical components; 3 identification of technological knowledge and policy demands; 4 identification of technological supply and 5 identification of research and policy projects. The agroalimentary value chain focus was applied and two consulting forums to identify demands and chain links were realized. Trough bibliographical consultation the technological supply was integrated, and when the supply was contrasted with the demand, the chain

  7. CADENA DEL CAFÉ DE LOS SANTOS, COSTA RICA

    Directory of Open Access Journals (Sweden)

    Carlos Francisco Carranza

    2012-05-01

    Full Text Available Resumen La operación de las actividades económicas y su competitividad, analizadas desde una perspectiva de cadenas globales de mercancías, dependen del funcionamiento de los mercados de crédito, tanto nacionales como internacionales. La operación normal de los negocios requiere crédito para fortalecer sus recursos propios y hacer las compras de materia prima, insumos, pago de salarios e inversión. En todos los casos, el crédito puede ser utilizado para mejorar el diseño de productos, impulsar el acceso a nuevos mercados y aumentar la competitividad. A los productores de la región de Los Santos en Costa Rica se les reconoce por innovadores, y al café de la zona por ser un café de altura y de gran calidad. El artículo explora las necesidades de financiamiento de productores, beneficiadores y exportadores de café de Los Santos, sus fuentes de financiamiento y el uso dado a los recursos obtenidos. Entre las necesidades orientadas al mejoramiento de la competitividad se encontraron la renovación de cafetales entre los productores, el mantenimiento de proyectos y tecnología entre los beneficios y la generación de microbeneficios y búsqueda de nichos de mercado entre los exportadores. Abstract From a global commodity chains perspective, economic activities and their competitiveness depend upon the functioning of credit markets, both international and domestic. Current businesses operations require credit to enhance their own resources in order to buy raw material and inputs, pay wages and make investment. Credit may also be used to improve product design, enter new markets and boost competitiveness as a result. High altitude grown coffee from Los Santos, Costa Rica is widely known for its quality, and their growers as innovative. This paper addresses credit needs of coffee growers, processors and exporters in Los Santos, the credit sources they have access to, and the kind of uses the credit is given. Improving competitiveness uses

  8. RESEÑA DEL SOFTWARE DISPONIBLE EN COLOMBIA PARA LA GESTIÓN DE INVENTARIOS EN CADENAS DE ABASTECIMIENTO

    Directory of Open Access Journals (Sweden)

    VALENTINA GUTIÉRREZ

    2009-01-01

    Full Text Available La gestión de los diferentes tipos de inventario a lo largo de una cadena de abastecimiento es un problema complejo que, en la mayoría de los casos de la industria colombiana e internacional, se aborda mediante la implementación de herramientas de software. Sin embargo, la decisión de implementar dichas herramientas está en muchos casos limitada por la capacidad financiera para invertir en ellas y por el desconocimiento de la oferta de software disponible en Colombia. Este artículo presenta una reseña del software disponible en Colombia para la gestión de inventarios con el fin de brindar a las empresas que se enfrentan a la decisión de implementar un software, una herramienta de soporte que indique cuál es la oferta en Colombia y cuáles son las características de los sistemas disponibles. Para esto se presenta primero el estado del arte, en el cual se identifica el resultado de la revisión de la literatura y el uso de las herramientas de software en la industria colombiana. Seguidamente se presenta la caracterización de los productos de software disponibles en el país. Dicha caracterización permite identificar la configuración general de los productos, los módulos de gestión con los que cuenta, las opciones de integración con otras herramientas informáticas, y la información general de precios e instalación. Finalmente se presentan las conclusiones del estudio y las futuras oportunidades de investigación.

  9. ALOE EN VENEZUELA: DE LA CADENA DE VALOR AL DISTRITO INDUSTRIAL

    Directory of Open Access Journals (Sweden)

    Henri Jesús Piña Zambrano

    2010-01-01

    Full Text Available Desde la perspectiva del estudio de los conglomerados, se contrastaron las aportaciones teóricas acerca de los conglomerados y el funcionamiento y desempeño de la cadena del aloe en el Estado Falcón (Venezuela, partiendo de la hipótesis de que esta cadena ha seguido un patrón evolutivo sustentado en el legado histórico de recursos que han favorecido la siembra y explotación del aloe en el estado. Sin embargo, se considera un escaso aprovechamiento de este patrimonio como estrategia para conformar un eficiente y competitivo conglomerado agroindustrial. La investigación se sustenta en estudios realizados entre 2003 y 2007 en las zonas productoras del aloe. Se identifican en la cadena los elementos típicos de un conglomerado (proximidad geográfica, redes, innovación e institucionalidad bajo un patrón organizativo que no promueve la consolidación del conglomerado. En la cadena del aloe no termina de crearse la atmósfera industrial necesaria para iniciar la districtualización de esta actividad productiva.

  10. Normalización de un sistema de reacción en cadena de la polimerasa en tiempo real para la cuantificación de papilomavirus humano de alto riesgo oncogénico

    Directory of Open Access Journals (Sweden)

    Yudira Soto

    2012-04-01

    Full Text Available El objetivo de este trabajo fue normalizar e implementar un sistema de reacción en cadena de la polimerasa en tiempo real, para determinar la carga viral de 7 genotipos de papilomavirus humano (PVH de alto riesgo oncogénico. Se evaluó la especificidad del sistema y se construyeron las curvas estándar para PVH 16 y 18, que se emplearon para la cuantificación de ADN viral en diferentes muestras de pacientes identificados como positivos a PVH, mediante reacción en cadena de la polimerasa (RCP cualitativa y secuenciación nucleotídica. Se obtuvieron dos curvas estándar para PVH 16 y 18, a partir del ADN genómico de las líneas celulares SiHa y HeLa, las que mostraron una buena correlación lineal ( r = -0,99 y valores bajos de error. El límite inferior de detección a partir del ADN de las líneas celulares fue de hasta 10 copias para ambos genotipos. No se obtuvo reacción cruzada entre los diferentes tipos de PVH ni con otros virus ADN. La reacción en cadena de la polimerasa en tiempo real (RCP-TR normalizada probó ser un sistema simple, rápido, específico y altamente sensible. Además, permitirá desarrollar investigaciones sobre la prevalencia de infección por PVH en Cuba, con vistas a la aplicación de las vacunas que se encuentran disponibles en el mercado internacional, así como la evaluación de otros candidatos vacunales diseñados en el futuro.

  11. Efecto de las decisiones de pedidos de clientes mayoristas en cadenas de abastecimiento: Un análisis experimental.

    OpenAIRE

    Villa Betancur, Sebastián

    2011-01-01

    Uno de los problemas más conocidos y costosos en las Cadenas de Abastecimiento se puede presentar ante la escasez de un producto en algún eslabón de la cadena. Este problema es caracterizado por el aumento de la variabilidad en las órdenes a medida que éstas se mueven a lo largo de la cadena. En esta tesis se investiga la forma en que un cliente intermedio (mayorista), enfrentando aumentos en la demanda del cliente final (minoristas), realiza sus pedidos a su único proveedor. Este proveedor c...

  12. La calidad ambiental como variable estratégica en cadenas agroalimentarias con tecnologías continuas

    OpenAIRE

    Jiménez, Gerardo; Pelupessy, Wim

    2009-01-01

    El objetivo de este artículo es desarrollar una metodología que permita evaluar las ventajas del mejoramiento estratégico de la calidad ambiental a lo largo de las cadenas agroalimentarias con tecnologías continuas. El desarrollo metodológico se lleva a cabo complementando el enfoque de las Cadenas Globales de Mercancías (CGM) con la teoría de juegos. En el artículo se hace una aplicación a la cadena global del café de Costa Rica. Específicamente el análisis se concentra en las potencialidade...

  13. Hackeando los ciber-riesgos en la cadena de suministro: Para entender los controles correctos, hay que mirar el sistema

    DEFF Research Database (Denmark)

    Sepúlveda Estay, Daniel Alberto; Khan, Omera

    2016-01-01

    Si se consideran tanto la creciente complejidad de las redes de abastecimiento, como la consecuente exposición de estas redes a interrupciones inesperadas ocasionadas por ciber-ataques, se requiere una forma más amplia para entender los ciber-riesgos en las cadenas de suministro. En este artículo...... teoría de control, y se enuncia una nueva forma de entender la resiliencia en la cadena de suministro. Nuestro trabajo propone un cambio foco desde la confiabilidad de los componentes individuales en la cadena de suministro, hacia el control y un entendimiento más profundo del sistema....

  14. Coordinación de agentes en cadenas de suministro descentralizadas, caso de estudio sector panificador, Palmira, Valle

    OpenAIRE

    Cárdenas Barbosa, Iván Darío

    2013-01-01

    En la presente tesis de maestría se desarrolla una propuesta metodológica para el modelado de cadenas de suministro desde un enfoque en el cual se consideren aspectos que tradicionalmente no se tienen en cuenta y que cobran mayor relevancia en un entorno de pequeñas empresas: reglas de negociación entre actores de la cadena, factores humanos presentes y la relevancia de la experiencia adquirida. El desarrollo de este proyecto incluye caracterización de una cadena de suministro del sector pani...

  15. Gobernanza en las Cadenas Globales de mercancías/valor: una revisión conceptual

    OpenAIRE

    Rafael Díaz Porras; Jorge Andrey Valenciano Salazar

    2012-01-01

    ResumenEn este documento se analiza el sistema de gobernanza de las cadenas de mercancías/valor a efectos de establecer los elementos conceptuales que se requieren para su análisis y derivar consecuencias analíticas para estudios que se realicen con la intención de mejorar la competitividad de actores participantes en los segmentos periféricos de las cadenas, desde países en vías de desarrollo. El análisis se efectúa siguiendo el enfoque de cadenas globales de mercancías y su evolución hacia ...

  16. ¿Cómo aplicar la flexibilidad administrativa en las entidades de educación superior mediante el sistema de costos abc?

    OpenAIRE

    Dilia Castillo

    2005-01-01

    El método de Costos Basado en Actividades se ha creado para generar en las organizaciones, estructuras administrativas flexibles mediante la clasificación de las actividades (cadena valor), en centros primarios y de apoyo. Este sistema permite la acumulación de costos en forma directa en cada dependencia responsable de desarrollar tareas que enconjunto originan que una organización ofrezca a sus clientes un producto terminado o un servicio eficiente a un costo real previamente determinado. La...

  17. Rearreglos de genes de cadenas pesadas de las inmunoglobulinas en las gammapatías monoclonales Immunoglobulin heavy chain gene rearrangements in the monoclonal gammopathies

    Directory of Open Access Journals (Sweden)

    Andrea Bosaleh

    2005-06-01

    Full Text Available Las neoplasias de células plasmáticas resultan de la expansión de un clon de células B que secreta inmunoglobulinas, conocido como componente monoclonal o componente M. Las neoplasias malignas incluyen al mieloma múltiple y la macroglobulinemia de Waldenström, y la condición premaligna comprende las gammapatías monoclonales de significado incierto (MGUS. El MGUS presenta un componente monoclonal sin evidencia de mieloma múltiple, macroglobulinemia de Waldenström, amiloidosis primaria u otros desórdenes. El diagnóstico se basa en la combinación de características patológicas, radiológicas y clínicas. Aproximadamente el 25% de las gammapatías monoclonales de significado incierto desarrollarán mieloma múltiple, amiloidosis sistémica, macroglobulinemia o enfermedades linfoproliferativas malignas, indicando que sería una condición premielomatosa. El objetivo del presente trabajo es establecer la utilidad clínica de la inmunofenotipificación por citometría de flujo (CF y la detección de clonalidad por biología molecular. Se estudiaron 32 pacientes, siete con diagnóstico de mieloma múltiple y veinticinco con gammapatía monoclonal en estudio, los cuales fueron divididos en cuatro grupos basados en los datos clínicos y los resultados de CF. En el grupo de pacientes con CF no diagnóstica, se realizó la detección de los rearreglos de los genes de las cadenas pesadas de las inmunoglobulinas mediante reacción en cadena de la polimerasa (PCR, detectándose monoclonalidad en el 59% de los casos. El estudio de los rearreglos de los genes de las cadenas pesadas de las IgH mediante PCR incrementa la sensibilidad de detección de monoclonalidad.Plasma cell neoplasia occurs as a result of the expansion of an immunoglobulin-secreting B-cells clones, known as monoclonal component or M component. Malignant neoplasias include multiple myeloma and Waldenström macroglobulinemia, while premalignant conditions comprise monoclonal

  18. Gobernanza en las Cadenas Globales de mercancías/valor: una revisión conceptual

    Directory of Open Access Journals (Sweden)

    Rafael Díaz Porras

    2012-07-01

    Full Text Available ResumenEn este documento se analiza el sistema de gobernanza de las cadenas de mercancías/valor a efectos de establecer los elementos conceptuales que se requieren para su análisis y derivar consecuencias analíticas para estudios que se realicen con la intención de mejorar la competitividad de actores participantes en los segmentos periféricos de las cadenas, desde países en vías de desarrollo. El análisis se efectúa siguiendo el enfoque de cadenas globales de mercancías y su evolución hacia el concepto de cadenas de valor. Palabras claves: Cadenas de valor, cadenas globales de mercancías, gobernanza, competitividad. AbstractThis paper analyzes the governance system of commodity chains in order to establish the conceptual elements that are required for the compression and analysis of this methodology. In addition, some analytical implications are proposed for studies intended to improve the competitiveness of actors participating in the peripheral segments of the chains from developing countries. The analysis has been conducted under the focus of global commodity chains and their evolution towards the concept of value chains. Keywords: Value chains, global commodity chains, governance, competitiveness.

  19. La cadena de valor: Una herramienta del pensamiento estratégico

    Directory of Open Access Journals (Sweden)

    Johana Quintero

    2006-09-01

    Full Text Available El instrumento más utilizado para realizar un análisis que permita extraer implicaciones estratégicas para el mejoramiento de las actividades es la Cadena de Valor, la cual identifica el valor para los clientes, fuente confiable de la ventaja competitiva. El Pensamiento estratégico bajo la perspectiva del Enfoque de Morrisey (1985 es intuitivo y está muy ligado al arte, tiene que ver con el punto de vista del estratega de los negocios. Esto permitió concluir que el desempeño superior se logrará a través de la cadena de valor, práctica que desde el punto de vista competitivo que contiene un carácter estratégico fundamental.

  20. Talento verde y cadenas de suministro verdes: ¿existe una relación significativa?

    Directory of Open Access Journals (Sweden)

    María del Carmen Torres-Salazar

    2016-01-01

    Full Text Available Introducción: En este texto se presentan resultados estadísticos de la relación entre directivos con características de talento verde y la implementación de prácticas de cadena de suministro verde. Debido a que existen investigaciones sobre la importancia de la visión de la alta dirección para la adopción de prácticas verdes; y sin embargo ninguna da cuenta de las características personales del directivo, ni tampoco de la relación significativa entre estas características y la puesta en marcha de prácticas de cadena de suministro verde, esta es la aportación de la presente investigación. El trabajo se desarrolló en empresas manufactureras con más de diez trabajadores de la zona oriente del estado de Morelos México. La caracterización del término talento verde se reportó en el artículo, Talento verde: caracterización y búsqueda, publicado en el número 14 de esta revista. Método: El método utilizado consistió en la construcción de una encuesta dividida en tres categorías que posteriormente se convirtieron en variables: prácticas de cadena de suministro verde, presiones institucionales y talento verde. Las dos primeras se adaptaron de la encuesta de Zhu y Sarkis (2007 y la tercera surgió de la representación social de talento verde, producto de una fase de esta investigación. La encuesta se validó con un grupo de empresarios miembros de la Cámara Nacional de la Industria de la Transformación (CANACINTRA capítulo Morelos. El instrumento validado se aplicó a 26 directivos de empresas manufactureras con más de diez trabajadores de la zona oriente de Morelos, con el fin de probar la relación entre directivos con características de talento verde y la puesta en marcha de prácticas de cadena de suministro verde. Para ello se utilizaron como herramientas estadísticas la correlación de Pearson y la regresión lineal múltiple. Resultados: Las prácticas de cadena de suministro verde de las empresas

  1. Cadena de suministro en el sector minero como estrategia para su productividad

    OpenAIRE

    CORREA ESPINAL, ALEXANDER; Rodrigo A. Gómez Montoya

    2009-01-01

    Este artículo de revisión bibliográfica busca identificar  la utilización de los modelos de cadena de suministro en el sector minero colombiano y suramericano como medio para aumentar la productividad a través de la revisión de estudios, artículos, libros y revistas. Para esto se parte, de la revisión de las generalidades de la minería en Suramérica y Colombia pasando por la descripción de los conceptos de cadena de suministro y su gestión, incluyendo la revisión de sus aplicaciones en Perú, ...

  2. Inventarios colaborativos en la optimización de la cadena de suministros

    Directory of Open Access Journals (Sweden)

    Martin Dario Arango-Serna

    2013-01-01

    Full Text Available La colaboración entre las partes de la cadena de suministro es una de las áreas de estudio más prometedoras, tanto para el entorno académico como para el empresarial, impulsada por los benefi cios que las empresas y las cadenas de suministro pueden obtener a partir de esta. Este artículo presenta una revisión de la colaboración en el manejo de inventarios, la cual es una de las estrategias más importantes para intentar reducir costos, mejorar el nivel de servicio y satisfacer a los clientes. El artículo realiza una revisión de los modelos de inventario colaborativos encontrados en la literatura científi ca, analiza los obstáculos mas frecuentes para su aplicación y propone algunas alternativas para eliminar dichos obstáculos.

  3. La Cadena de Suministro de la Energía Solar

    Directory of Open Access Journals (Sweden)

    Virginia Guadalupe López Torres

    2012-01-01

    Full Text Available Se presenta la cadena de suministro instalada en México para el mercado de la energía solar, con base a una investigación exploratoria realizada en bases de datos especializadas de empresas dedicadas a la fabricación, proveeduría, comercialización y prestación de servicios de sistemas solares. Los resultados indican la no existencia en México de una cadena de suministro articulada, sólo se perciben eslabones aislados, por ello se plantea que el gobierno a través de políticas públicas fomente su desarrollo a fin de fortalecer este segmento de mercado.

  4. Tegnologías de la información en la cadena de suministro

    OpenAIRE

    ALEXANDER CORREA ESPINAL; RODRIGO ANDRÉS GÓMEZ MONTOYA

    2009-01-01

    Este artículo de revisión bibliográfica busca identificar el estado del arte y la utilización de las Tecnologías de la Información y Comunicaciones (TIC’s) en la cadena de suministro y su nivel de aplicación en la industria colombiana a través del análisis de algunos estudios existentes. Para esto se parte de conceptos generales, hasta llegar a las TIC’s logísticas, las cuales varían según las características y necesidades de la cadena de suministro. A partir de dicha revisión se observó ...

  5. La Inteligencia Institucional y Cadena de Valor de la Información: El Ejemplo de la Universidad Federal de Rio Grande do Norte - Brasil

    Directory of Open Access Journals (Sweden)

    Márcia Josienne Monteiro Chacon

    2014-12-01

    Full Text Available La transformación del progreso científico y tecnológico lleva a la rápida difusión de conocimientos, es la era de los datos. La inteligencia de negocios tiene cómo objetivos recoger datos para la toma de decisiones. El término Inteligencia Institucional es adecuado para su uso en la Universidad. La realización de la cadena de valor son las unidades administrativas. Así, los problemas que amenazan en los servicios de TI, tiene su origen en la aplicación inadecuada. En los aspectos de la gestión no se puede olvidar que los usuarios finales, puesto que mediante el uso efectivo de los productos de datos pueden generar valor para la Universidad. Se sabe que hay limitaciones y el impacto que pueden tener en la iniciativa tiende a bajar. Recomendamos más investigaciones porque no es nuestra intención de agotar el tema, sino presentar algunos conceptos que impulsan el proceso.

  6. La Inteligencia Institucional y Cadena de Valor de la Información: El Ejemplo de la Universidad Federal de Rio Grande do Norte - Brasil

    Directory of Open Access Journals (Sweden)

    Márcia Josienne Monteiro Chacon

    2014-11-01

    Full Text Available La transformación del progreso científico y tecnológico lleva a la rápida difusión de conocimientos, es la era de los datos. La inteligencia de negocios tiene cómo objetivos recoger datos para la toma de decisiones. El término Inteligencia Institucional es adecuado para su uso en la Universidad. La realización de la cadena de valor son las unidades administrativas. Así, los problemas que amenazan en los servicios de TI, tiene su origen en la aplicación inadecuada. En los aspectos de la gestión no se puede olvidar que los usuarios finales, puesto que mediante el uso efectivo de los productos de datos pueden generar valor para la Universidad. Se sabe que hay limitaciones y el impacto que pueden tener en la iniciativa tiende a bajar. Recomendamos más investigaciones porque no es nuestra intención de agotar el tema, sino presentar algunos conceptos que impulsan el proceso.

  7. LA POLÍTICA DE DEVOLUCIÓNES EN LAS GRANDES CADENAS DE DISTRIBUCIÓN PRESENTES EN SAN JOSÉ DE CÚCUTA COMO MODELO DE FIDELIZACIÓN DEL CLIENTE

    Directory of Open Access Journals (Sweden)

    Saúl Villalobos Marín

    2016-02-01

    Full Text Available Resumen La presente investigación tiene como objetivo determinar la política de devoluciones en las principales cadenas de distribución presentes en la ciudad de San José de Cúcuta como estrategia de fidelización del cliente y su implementación, mediante la creación de un modelo para las pequeñas y medianas empresas de la ciudad. Para dar el cumplimiento a este propósito, se realizó un estudio explicativo ya que no sólo persigue describir o acercarse al problema, sino que intenta encontrar las causas del mismo y proponer una posible estrategia para su solución. Para ello se realizó un estudio a todo el proceso que involucra la gestión de las devoluciones para analizar el contexto de esta estrategia en las principales cadenas de distribución de la ciudad. El estudio se realizó mediante una encuesta y entrevista personal a los responsables de las políticas de fidelización y/o devoluciones. Creándose posteriormente un modelo de política de devoluciones basado en las conclusiones extraídas y teniendo en cuenta los antecedentes nacionales e internacionales. Se evidenció la relación entre la política de devoluciones y la fidelización del cliente, se pudo reflejar la opinión altamente positiva que tienen los responsables de los establecimientos de este tipo de estrategias. Se dejó patente que las empresas que aplican una estrategia enfocada a la satisfacción del cliente obtienen mejores resultados en ventas y crecimiento empresarial respecto a las grandes cadenas que despreocupan esta parte. Se pudo mostrar como las estadísticas y la opinión de los gerentes demuestra que es rentable para la empresa en términos económicos, la aplicación de este tipo de políticas y como una empresa con una política de devoluciones clara y bien definida sale beneficiada y reforzada en su imagen de marca, percepción de los clientes y estabilidad en las ventas para poder afrontar periodos económicos adversos como crisis económicas o

  8. Kinetic ELISA in Microfluidic Channels

    Directory of Open Access Journals (Sweden)

    Debashis Dutta

    2011-06-01

    Full Text Available In this article, we describe the kinetic ELISA of Blue Tongue and Epizootic Hemorrhagic Disease viral antibodies in microfluidic channels by monitoring the rate of generation of the enzyme reaction product under static conditions. It has been shown that this format of the immunoassay allows very reliable quantitation of the target species using inexpensive glass microchips and a standard epifluorescence microscope system coupled to a CCD camera. For the viral antibodies assayed here, the limit of detection (LOD for the analyte concentration in our microchips was established to be 3–5 times lower than that obtained on commercial microwell plates using a fiftieth of the sample volume and less than a third of the incubation time. Our analyses further show that when compared to the end-point ELISA format, the kinetic mode of this assay yields an improvement in the LOD by over an order of magnitude in microfluidic devices. This benefit is primarily realized as the observed variation in the background fluorescence (signal at the start of the enzyme reaction period was significantly larger than that in the rate of signal generation upon repeating these assays in different microchannels/microchips. Because the kinetic ELISA results depend only on the latter quantity, the noise level in them was substantially lower compared to that in its end-point counterpart in which the absolute fluorescence measurements are of greater significance. While a similar benefit was also recorded through implementation of kinetic ELISAs on the microwell platform, the improvement in LOD registered in that system was not as significant as was observed in the case of microfluidic assays.

  9. A quantitative ELISA for dystrophin.

    Science.gov (United States)

    Morris, G E; Ellis, J M; Nguyen, T M

    1993-05-01

    A novel approach to the quantitation of the muscular dystrophy protein, dystrophin, in muscle extracts is described. The two-site ELISA uses two monoclonal antibodies against dystrophin epitopes which lie close together in the rod domain of the dystrophin molecule in order to minimize the effects of dystrophin degradation. Dystrophin is assayed in its native form by extracting with non-ionic detergents and avoiding the use of SDS. PMID:8486926

  10. Modelo de integración de cadenas de suministro colaborativas

    Directory of Open Access Journals (Sweden)

    Horacio Bautista-Santos

    2015-01-01

    Full Text Available Los integrantes estratégicos de las cadenas de suministro colab orativas han buscado realizar acciones conjuntas que les permit an sincronizar los eslabones, para mejorar el ser vicio al cliente y hacer fren te a los retos de competitividad globalizada del entorno actual ; esta tendencia se conoce como integración de la cadena de sumin istro y permite mejorar el desempeño de las empresas en su conjunto. En este t rabajo se presenta un modelo de integración de cadenas de suministro cola borativas considerando las perspe ctivas estratégica, táctica y operativa. Para su implementación se diseñ ó un instrumento de medición, el cual fue validado estadísticamente y se formuló un modelo mate mático utilizando lógica difusa para su resolución. El modelo se aplic ó en 36 empresas mexicanas entre pequeñas, medianas y grandes, determinando su nivel de integración, con base en los resultado s se propusieron acciones específicas a implementar para mejora r el nivel alcanzado.

  11. TEGNOLOGÍAS DE LA INFORMACIÓN EN LA CADENA DE SUMINISTRO

    Directory of Open Access Journals (Sweden)

    ALEXANDER CORREA ESPINAL

    2009-01-01

    Full Text Available Este artículo de revisión bibliográfica busca identificar el estado del arte y la utilización de las Tecnologías de la Información y Comunicaciones (TIC's en la cadena de suministro y su nivel de aplicación en la industria colombiana a través del análisis de algunos estudios existentes. Para esto se parte de conceptos generales, hasta llegar a las TIC's logísticas, las cuales varían según las características y necesidades de la cadena de suministro. A partir de dicha revisión se observó que estas tecnologías en su mayoría contribuyen a la reducción de costos y a mejorar el flujo de información entre los actores de la misma. Los principales obstáculos para la implementación de las TIC's en la cadena de suministro son los altos costos, la cultura de la organización y la inadecuada estructuración de los procesos. Con relación a su uso en Colombia, se identificó en los estudios analizados, un bajo grado de implementación en las pequeñas y medianas empresas (PYMES y un nivel medio en las grandes empresas y operadores logísticos.

  12. CARACTERIZACIÓN DE LA FASE UPSTREAM DE LA CADENA DE VALOR Y ABASTECIMIENTO DE LA AGROINDUSTRIA DE LA PALMA DE ACEITE EN COLOMBIA

    Directory of Open Access Journals (Sweden)

    Rafael García Cáceres

    2013-01-01

    Full Text Available Este artículo proporciona una descripción de la fase upstream de la cadena agroindustrial de la palma de aceite. El trabajo caracteriza la funcionalidad y relaciones de eslabones y escalones de la cadena de abastecimiento y de valor de la cadena en Colombia. El trabajo representa un esfuerzo pionero que busca contribuir a mejorar la eficiencia y eficacia del sector palmero colombiano.

  13. Canine specific ELISA for coagulation factor VII

    DEFF Research Database (Denmark)

    Knudsen, Tom; Kjelgaard-Hansen, Mads; Tranholm, Mikael;

    2011-01-01

    available to date. In this study, a canine specific ELISA for measurement of FVII:Ag in plasma was developed and validated. The FVII:Ag ELISA correctly diagnosed homozygous and heterozygous hereditary FVII deficiency. Together with activity based assays, such as FVII:C, the FVII:Ag ELISA should be valuable...

  14. Publicidad expandida mediante realidad aumentada

    OpenAIRE

    Martí Parreño, José

    2011-01-01

    La realidad aumentada, aplicada al marketing, abre numerosas oportunidades para propiciar la decisión de compra del consumidor. Éste puede ver cómo un producto “cobra vida” mediante una pantalla en la que se superponen imágenes e información digital a la del entorno real que está viendo en ese mismo momento. En España, los consumidores ya han podido ver cómo empresas como El Corte Inglés o Doritos empleaban esta novedosa tecnología para promocionar sus productos. El autor plantea, por tanto, ...

  15. “ANÁLISIS DE LA CADENA AGROALIMENTARIA DE CONSERVAS DE TOMATE NATURAL EN LA PROVINCIA DE MATANZAS”

    Directory of Open Access Journals (Sweden)

    Neyfe Sablón Cossío

    2015-01-01

    Full Text Available El presente artículo tiene como objetivo principal, analizar la cadena agroalimentaria de conservas de tomate natural en la provincia de Matanzas. En la actualidad existen deficiencias en el abastecimiento de alimentos en las cadenas cubanas, se hace necesario mostrar una solución inmediata para elevar la producción y disminuir las brechas en la logística que une la producción primaria con las redes comercializadoras, para ofrecer los alimentos frescos o procesados a los consumidores. En este trabajo se presenta un estudio del procedimiento y posteriormente como se aplica en la red de conservas de tomate natural en la ciudad de Matanzas. En general, el enfoque en cadenas agroalimentarias impacta de forma positiva en la satisfacción de los clientes. Como aporte económico fundamental, la mejora de la disponibilidad de productos como indicador base del funcionamiento de una cadena de suministro, conlleva a la elevación de las ventas en la red de mercados. Elevar la satisfacción de las necesidades alimentarias de la población y facilitar el trabajo de apropiación que deben realizar los consumidores para adquirir alimentos frescos en el territorio, constituye el aporte social del análisis de las cadenas de suministro

  16. Capacidad discriminatoria y concordancia entre el ELISA-F29 y la PCR en individuos con infección por T. cruzi / Discriminatory Power and Concordance between ELISA-F29 and PCR in Individuals with Infection due to T.cruzi / Capacidade discriminatória e acordo entre o ELISA-F29 e a PCR em indivíduos infectados com T. cruzi

    Directory of Open Access Journals (Sweden)

    Edgar David Gómez-Laitón, MD.

    2015-07-01

    Full Text Available Introducción: El diagnóstico de la infección por Trypanosoma cruzi (T. cruzi se realiza rutinariamente mediante pruebas serológicas mientras que el empleo de métodos moleculares se encuentra aún en proceso de estandarización. Objetivo: Evaluar la capacidad discriminatoria y concordancia entre una prueba serológica y una molecular para determinar la infección por T. cruzi. Métodos: Se realizó Reacción en Cadena de la Polimerasa (PCR y la prueba de ELISA-F29 en 95 muestras de participantes de la cohorte “Cardiovascular health investigation and collaboration countries of America to assess the markers and outcomes of Chagas disease” CHICAMOCHA. Se evaluó la capacidad discriminatoria del ELISA-F29 respecto al resultado de PCR mediante la estimación del área bajo la curva ROC. Se estimó la tasa de falsos positivos al 25% y sensibilidad al 75%. Se determinó la concordancia mediante kappa de Cohen. Resultados: Se realizaron pruebas de PCR en dos momentos diferentes en 95 individuos (edad media: 38 años; 64% hombres, con tasas de positividad entre 1.1% – 2.2% para los primers S35-S36 y entre 18.3% – 34.7% para los primers 121-122, respectivamente. La capacidad discriminatoria del ELISA- F29 respecto a PCR fue 0.62 (IC95%: 0.53; 0.70 y tasa de falsos positivos del 56% (IC95%: 42; 70. El punto de corte óptimo para el cociente de absorbancia fue 2.53 (sensibilidad 59% y especificidad 60%. Para el primer 121-122 los niveles de acuerdo observado y kappas estimados fueron: 52.6% y 0.10 (IC95%: -0.08, 0.28 para la primera medición, 62.4% y 0.09 (IC95%: -0.09, 0.28 para la segunda medición y 57.5% y 0.13 (IC95%: 0.01, 0.26 al evaluar simultáneamente las dos mediciones. Conclusiones: Los resultados demuestran una baja concordancia evidenciada por los valores de kappa determinados en el estudio. Es necesario afinar los estudios para evaluar la utilidad de las pruebas moleculares en el diagnóstico de la Enfermedad de Chagas. [G

  17. Las relaciones en la cadena de suministro no son tan peligrosas

    OpenAIRE

    Teresa Vallet-Bellmunt

    2010-01-01

    Este trabajo analiza cómo las relaciones en la cadena de suministro, a través de la estrategia de integración, pueden ser beneficiosas para sus miembros en términos de resultados logísticos, de marketing e incluso corporativos. Se estudia, a través de los “arcos de integración”, la influencia tanto de la amplitud como de la dirección de la integración (aguas arriba o abajo) en una muestra de 99 empresas pertenecientes a un distrito industrial. Se ofrece evidencia de que el arco...

  18. Fases de la cadena de suministro de las empresas avícolas

    OpenAIRE

    Bertila J. Aponte F.; Abraham J. González; Angel D. González P.

    2013-01-01

    En la cadena de suministro de las empresas avícolas se realiza una gran cantidad de procesos que originan una brecha de tiempo y espacio entre el insumo y el producto final, dificultando el adecuado análisis de sus elementos. Por esto, el objetivo del presente artículo es analizar las fases de la misma, específicamente en lo correspondiente a la producción de pollo. La investigación fue de tipo analítica, no experimental; con un diseño transeccional. La muestra estuvo conformada por 4 e...

  19. Impacto de infraestructura de calidad en la cadena láctea

    OpenAIRE

    Claudia Santo; Elizabeth Ferreira

    2011-01-01

    Las acciones realizadas en forma sistémica por los diferentes actores de la infraestructura de la calidad a nivel nacional han potenciando la calidad a lo largo de toda la cadena productiva y logrado los efectos esperados de mejora de los productos lácteos en el país para su mejor introducción en el mercado internacional.En el sector lácteo uruguayo, los actores de la infraestructura de la calidad son el Ministerio de Ganadería, Agricultura y Pesca (MGAP), en su papel de reglamentador y certi...

  20. Indicadores logísticos en la cadena de suministro como apoyo al modelo scor

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    Abdul Zuluaga Mazo

    2015-01-01

    Full Text Available Este artículo tiene como principal objetivo revisar, analizar y proponer el uso de indicadores en los diferentes procesos logísticos de la cadena de suministro, los cuales cubren desde el aprovisionamiento pasando por el almacenamiento, la producción, el servicio al cliente, entre otros. Como resultado del desarrollo de un trabajo de investigación llamado « Estrategias logísticas para el abastecimiento de las pymes del sector confección del municipio de Itagüí». Se concluye que los indicadores planteados permiten medir el desempeño de los diferentes procesos logísticos en la cadena de suministro, lo cual, se convierte en la base para control del uso de los recursos, seguimiento al cumplimiento de objetivos e identificación de oportunidades de mejoramiento. La metodología que se utilizó para definir los indicadores fue a través del análisis de bibliografía especializada y la creación de unos indicadores propios a partir de modelos de referencia estándar de algunas empresas. Adicionalmente, los indicadores que se presentan sirven de apoyo a la medición del modelo de cadena de suministro SCOR, lo cual, se convierte en un valor agregado para el ámbito académico y empresarial.Palabras clave: Cadena de Suministro, nivel de desempeño, indicador, logística, KPI’s (Indicadores  de desempeño.Logisticals indicators in the supply chain as support to scor model.This paper has as its main objective of reviewing, analyzing and propose the use of indicators in different logistics processes  in the supply chain, which range from the supply, warehouse, production, customer service, among others. As a result of developing the document, supported by a previous research work called « Estrategias logísticas para el abastecimiento de las pymes del sector confección del municipio de Itagüí». We obtain indicators to measure the performance of the different logistics process in the supply chain, which becomes the basis to control the

  1. LAS CADENAS PRODUCTIVAS, ESTRATEGIA DE DESARROLLO EMPRESARIAL PARA EL MUNICIPIO DE TAMUÍN, SAN LUIS POTOSÍ

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    Ana Diana Betancourt Enríquez

    2013-08-01

    Full Text Available El presente artículo tiene como principal objetivo el análisis del esquema de cadena productiva, como estrategia para el desarrollo competitivo empresarial del municipio de Tamuín, S.L.P., aunado al caso del Grupo Gusi Sociedad de Producción Rural de Responsabilidad Limitada de esta localidad. En la investigación se presentan lineamientos teóricos que incluyen la conceptualización de la cadena productiva, clasificación de la misma, ventajas de integración del esquema, y rubros de lo local a lo global, donde se incorpora un acercamiento sobre el modelo endógeno dentro de la integración de las cadenas productivas.

  2. LAS CADENAS PRODUCTIVAS, ESTRATEGIA DE DESARROLLO EMPRESARIAL PARA EL MUNICIPIO DE TAMUÍN, SAN LUIS POTOSÍ

    OpenAIRE

    Ana Diana Betancourt Enríquez; Hugo Daniel Navarro Payta

    2013-01-01

    El presente artículo tiene como principal objetivo el análisis del esquema de cadena productiva, como estrategia para el desarrollo competitivo empresarial del municipio de Tamuín, S.L.P., aunado al caso del Grupo Gusi Sociedad de Producción Rural de Responsabilidad Limitada de esta localidad. En la investigación se presentan lineamientos teóricos que incluyen la conceptualización de la cadena productiva, clasificación de la misma, ventajas de integración del esquema, y rubros de lo local a l...

  3. Contratación y facturación electrónica en la cadena de valor

    OpenAIRE

    Afellat Oddi, Narjiss

    2011-01-01

    Las Tecnologías de la Información y de la Comunicación (TIC) están adquiriendo cada vez más importancia en el ámbito empresarial ya que permiten enlazar la cadena de valor de una empresa con las cadenas de valor de sus proveedores y clientes. En este sentido, las TIC adquieren un valor estratégico, transformando la manera en que se realizan las actividades de producción de valor y la naturaleza de los enlaces entre ellas. En este marco conceptual, la Contratación y la Facturación electrónicas...

  4. Financiación estructurada en la cadena productiva palma, aceites, grasas vegetales y biocombustibles en Colombia

    OpenAIRE

    Quintero Jaramillo, Camilo Eduardo

    2015-01-01

    Los principales hitos en proyectos de inversión en la cadena productiva denominada “palma, aceites, grasas vegetales y biocombustibles” en Colombia y su financiación no se encuentran acompasados con una oferta apropiada, la estructura de financiación ofrecida por el mercado no identifica la existencia de la cadena productiva o esta no se revela como tal ante el sistema financiero -- Haciendo una revisión del portafolio de productos y servicios ofrecido en la actualidad por el sistema financie...

  5. Ventajas competitivas: Una propuesta paso a paso de búsqueda y aplicación de cadena de valor adaptada a empresas aéreas. Caso Patagonia- Argentina

    Directory of Open Access Journals (Sweden)

    María Alejandra Gazzera

    2005-05-01

    Full Text Available El desarrollo del proceso de búsqueda de ventajas competitivas de una empresa se puede plantear como un esfuerzo que se sigue en tres grandes líneas de actuación: la búsqueda centrada en la empresa, centrada en el entorno, y centrada en los competidores. Si bien la búsqueda de ventajas competitivas está estrechamente interrelacionada y vinculada entre las tres líneas, el presente trabajo se centrará en la primera de ellas, es decir al interior de la empresa, sin perder de vista la relación que tienen las empresas con su entorno o mercado, mediante la aplicación de la cadena de valor de Michael Porter (1985 adaptada a las empresas aéreas que operaban desde y hacia la ciudad de Neuquen, puerta de entrada a la Patagonia Argentina Es necesario destacar que no existen al presente, año 2005, estudios de este tipo en el sector mencionado en Argentina, pero en otros sectores productivos y aún en empresas aéreas de otros países, la cadena de valor ha demostrado su utilidad como tecnología de reflexión estratégica para generar valor en el cliente y para dirigir los esfuerzos de la empresa hacia la consolidación o creación de ventajas competitivas sostenibles en el tiempo.

  6. Cadena del frío para la conservación de las vacunas en los centros de atención primaria de un área de Madrid: mantenimiento y nivel de conocimientos

    Directory of Open Access Journals (Sweden)

    Ortega Molina Paloma

    2002-01-01

    Full Text Available Fundamento. Las vacunas son medicamentos termolábiles y para garantizar su inmunogenicidad y eficacia protectora, dentro de los programas de inmunización, es imprescindible mantener la cadena de frío. El elemento fundamental en esta cadena es el personal responsable de las vacunas, que debe conocer las características de estabilidad de cada preparado con el fin de evitar errores durante su manipulación. El objetivo de este trabajo fue conocer cómo se realiza el mantenimiento de la cadena del frío en equipos de atención primaria de un área sanitaria de la Comunidad Autónoma de Madrid, así como establecer el grado de información que poseen los responsables de las vacunas con respecto a la termoestabilidad de las mismas. Métodos. Se ha realizado un estudio transversal en 46 puntos de vacunación en atención primaria. La recogida de los datos se realizó mediante entrevista personal por un único investigador. Resultados. La tasa de participación fue del 93,5% (43/46. En todos los casos existía termómetro de máxima y mínima y registro mensual de la temperatura. Se observó una temperatura inadecuada en tres ocasiones (6,97%. El porcentaje de profesionales que conocía el efecto que la congelación producía sobre las vacunas fue muy diverso: 53.5%, 51.2%, 44.2% y 53.5% para difteria-tétanos-pertussis (DTP, hepatitis B (VHB, polio oral (VPO y rubéola-sarampión-paperas (RSP respectivamente. Y sólo el 32% conocía el test de agitación. Conclusión. La formación de los profesionales sobre el efecto que las altas temperaturas ocasionan en las vacunas era correcta, pero es necesario reforzar su formación sobre la inestabilidad que presentan los preparados adsorbidos cuando se someten a congelación.

  7. Las relaciones en la cadena de suministro no son tan peligrosas

    Directory of Open Access Journals (Sweden)

    Teresa Vallet-Bellmunt

    2010-01-01

    Full Text Available Este trabajo analiza cómo las relaciones en la cadena de suministro, a través de la estrategia de integración, pueden ser beneficiosas para sus miembros en términos de resultados logísticos, de marketing e incluso corporativos. Se estudia, a través de los "arcos de integración", la influencia tanto de la amplitud como de la dirección de la integración (aguas arriba o abajo en una muestra de 99 empresas pertenecientes a un distrito industrial. Se ofrece evidencia de que el arco más amplio es el que obtiene mejores resultados. Todo ello nos permite proponer un marco de investigación futura que ayude a profundizar en el conocimiento de esta relación, así como una serie de implicaciones de gestión para que los directivos de las empresas no vean las relaciones en la cadena de suministro como peligrosas.

  8. Fases de la cadena de suministro de las empresas avícolas

    Directory of Open Access Journals (Sweden)

    Bertila J. Aponte F.

    2013-01-01

    adecuado análisis de sus elementos. Por esto, el objetivo del presente artículo es analizar las fases de la misma, específicamente en lo correspondiente a la producción de pollo. La investigación fue de tipo analítica, no experimental; con un diseño transeccional. La muestra estuvo conformada por 4 empresas avícolas del estado Zulia, siendo los gerentes informantes clave en la investigación. Los resultados indican que la fase de aprovisionamiento de la cadena de suministro avícola posee 9 eslabones que aplican alianzas comerciales, tecnológicas y de producción para mantenerse integrados. La fase de procesamiento está compuesta por 2 eslabones que operan bajo una estrategia de producción continua. Finalmente, la fase de distribución posee 4 eslabones, que conforman canales de distribución de varios intermediarios que distribuyen los productos de forma intensiva. Se concluye que la industria avícola posee una cadena de suministro compleja, formada por 15 eslabones que se encargan de producir, procesar y distribuir diferentes productos a los consumidores en el momento, cantidad y condiciones deseadas.

  9. Retiro y autogestión del usuario en almacenes de cadena con directorio activo

    Directory of Open Access Journals (Sweden)

    Juan F. Hincapié Z.

    2012-12-01

    Full Text Available Los almacenes de cadena son organizaciones en donde el personal es numeroso, diverso, de alta rotación y alto retiro; además la gran variedad de negocios que tienen tales organizaciones, hacen que posean muchas aplicaciones a las cuales muchos usuarios deben ingresar y en la mayoría de veces éste acceso se vuelve administrativamente costoso. Por tanto se hace necesario el establecimiento de un procedimiento informático (administración de usuarios que permita al propio usuario gestionar sus contraseñas y que además le permita a la organización controlar la desactivación automática de los accesos al momento del retiro (terminación de contrato del usuario. Para lograr esto existen variedad de herramientas en el mercado que satisfacen dicha necesidad, pero su implementación y soporte es demasiado costoso; por eso este artículo pretende mostrar algunos conceptos importantes y la manera en que otras herramientas y metodologías pueden realizar un adecuado proceso de autogestión y retiro del usuario en los almacenes de cadena a un menor costo.

  10. Assessment of an ELISA Laboratory Exercise

    Science.gov (United States)

    Robinson, David L.; Lau, Joann M.

    2012-01-01

    The enzyme-linked immunosorbent assay (ELISA) is a powerful immunological technique for quantifying small amounts of compounds and has been used in research and clinical settings for years. Although there are laboratory exercises developed to introduce the ELISA technique to students, their ability to promote student learning has not been…

  11. A Simple ELISA Exercise for Undergraduate Biology.

    Science.gov (United States)

    Baker, William P.; Moore, Cathy R.

    Understanding of immunological techniques such as the Enzyme Linked Immuno Sorbent Assay (ELISA) is an important part of instructional units in human health, developmental biology, microbiology, and biotechnology. This paper describes a simple ELISA exercise for undergraduate biology that effectively simulates the technique using a paper model.…

  12. Reconversión de la cadena agroindustrial de la caña de azúcar en Veracruz México

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    Noé Aguilar Rivera

    2014-01-01

    Full Text Available La agroindustria azucarera de Veracruz participa con 40 % de la producción nacional de sacarosa e integra actividades agrícolas de crecimiento, cosecha y transporte de caña de azúcar con la producción industrial en ingenios azucareros, destilerias y trapiches piloncilleros. Sin embargo, enfrenta retos con la caída de la productividad y aspectos socioeconómicos que ponen en riesgo la seguridad alimentaria y la reconversión de la agroindustria. El objetivo del presente trabajo es evaluar la capacidad y estrategias de reconversión de la agroindustria azucarera de Veracruz mediante el análisis de la productividad de la cadena agroindustrial en el contexto nacional con el uso de diversas herramientas de análisis como Matriz de ponderación de productividad en campo, ingenios azucareros y municipios productores, zonificación agroecológica y diamante de competitividad. Los resultados establecen que el 40 % de los ingenios azucareros poseen indicadores por encima de la media nacional. Las zonas de abasto cañero presentan en su conjunto 12 % menor productividad en relación a su potencial agroecológico, a pesar que en Veracruz el 57.5 % de su zona productora tiene aptitud edafoclimática al cultivo de media a alta y 30 municipios (33.7 % poseen recursos y capacidades para reconvertirse. Se concluye que las acciones y estrategias para incrementar la productividad para bioenergía y alimentos en Veracruz debe abordarse desde una perspectiva interdisciplinaria como una interfaz entre la optimización del rendimiento, la ecología, limitantes técnicos y socioeconómicos y la nutrición humana como una cuestión de eficiencia de los recursos disponibles.

  13. CONVOCATORIAS DE INVESTIGACIÓN, DESARROLLO TECNOLÓGICO E INNOVACIÓN POR CADENAS PRODUCTIVAS EN EL SECTOR AGROPECUARIO

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    B., A.M. Ramírez

    2008-01-01

    Full Text Available El Ministerio de Agricultura y Desarrollo Rural ha realizado entre 2004 y 2008 ocho convocatorias en las que seha recibido un total de 2.584 propuestas, en busca de cofinanciación para la ejecución de programas y proyec-tos de I+D+I. En 2004 se presentaron 64 proyectos para tres cadenas priorizadas y un área transversal, mientrasque en 2008 fueron 1.037 propuestas en veintinueve cadenas y tres áreas transversales. Entre 2004 y 2008 sehan contratado 591 proyectos en diferentes cadenas productivas, por valor de $452,3 mil millones, de los cualesse cofinancian $208,1 mil millones. Las cadenas productivas con el mayor número de proyectos aprobados ycontratados en las convocatorias I+D+I del Ministerio de Agricultura, son: frutales, con 112 proyectos; lácteos,con 47; forestal, con 40; hortalizas, 38; carne bovina, 36; piscicultura, 35, y papa con 36 proyectos. Estos resultados, presentados en diferentes áreas temáticas indican la necesidad de priorizar y focalizar esfuerzos y seguirapoyando este tipo de procesos, teniendo en cuenta que cada vez son más las propuestas que se reciben paraaplicar a las convocatorias de I+D+I.

  14. La investigación en gestión de la cadena de suministro a nivel mundial

    OpenAIRE

    Luz Mary Nova Caldas

    2010-01-01

    Este artículo analiza el estado del arte en relación con la investigación sobre gerencia de la cadena de suministro. Se hace particular énfasis en las variables estudiadas y los principales resultados obtenidos. Varias encuestas aplicadas alrededor del mundo son analizadas para identificar patrones en este tema.

  15. La investigación en gestión de la cadena de suministro a nivel mundial

    Directory of Open Access Journals (Sweden)

    Luz Mary Nova Caldas

    2010-07-01

    Full Text Available Este artículo analiza el estado del arte en relación con la investigación sobre gerencia de la cadena de suministro. Se hace particular énfasis en las variables estudiadas y los principales resultados obtenidos. Varias encuestas aplicadas alrededor del mundo son analizadas para identificar patrones en este tema.

  16. Acil coenzima-a sintetasas de cadena larga (acsls) como biomarcadores citoserológicos en enfermedades inflamatorias o autoinmunes

    OpenAIRE

    Matesanz, F; Fedetz, María; Ndagire, D.; Alcina, Antonio; Sabio, José Mario

    2010-01-01

    [ES] Uso de las Acil-Coenzima-A sintetasas de cadena larga (ACSL) como biomarcadores cito-serológicos de enfennedades inflamatorias o autoinmunes, y especialmente de lupus eritematoso sistémico, y método de obtención de datos útiles para el diagnóstico y seguimiento de dichas enfennedades.

  17. DIAGNÓSTICO MOLECULAR DE Campylobacter EN LA CADENA AVÍCOLA DESTINADA PARA CONSUMO HUMANO EN COSTA RICA

    Directory of Open Access Journals (Sweden)

    Leana Zumbado-Guti\\u00E9rrez

    2014-01-01

    Full Text Available Diagnóstico molecular de Campylobacter en la cadena avícola destinada para consumo humano en Costa Rica. El objetivo de este estudio fue determinar la presencia y la frecuencia de Campylobacter jejuni y Campylobacter coli, en la cadena de producción avícola en Costa Rica. Se evaluaron granjas, planta de proceso y puntos de venta del Gran Área Metropolitana. Para la detección y aislamiento de Campylobacter sp. se analizaron 84 muestras provenientes de la cadena avícola (24 muestras de carne de pollo tomadas en punto de venta, 20 enjuagues de carcasa y 40 de ciegos tomados en planta de beneficio, obtenidas en noviembre de 2012, siguiendo el protocolo ISO 10272- 1:2006 modificado por el Departamento de Agricultura de Estados Unidos (USDA, por sus siglas en inglés. De las 84 muestras analizadas, 36 (42,8% resultaron positivas para C. jejuni, y una (1,2% para C. coli. La cantidad de muestras positivas según la Reacción en Cadena de Polimerasa (PCR, por tipo de muestra fue: para contenido cecal, 16 (n=40, para carcasas, 8 (n=20 y para punto de venta 12 (n=24. Dieciséis de las veinte granjas muestreadas fueron positivas. Hubo una alta frecuencia de Campylobacter en todos los puntos de la cadena muestreados, lo que podría estar asociado a modificaciones de parámetros relacionados con inocuidad en cada eslabón. El enfriamiento rápido y el uso de cloro en el agua en la planta de proceso, disminuyó la frecuencia de muestras positivas de Campylobacter spp.

  18. Análisis y optimización de una cadena de suministro con logística inversa e incertidumbre en la demanda

    OpenAIRE

    Pereda Pons, Julian

    2014-01-01

    Este proyecto tiene como objetivo desarrollar, optimizar y analizar una cadena de suministro basada en una arquitectura multiagente que permite simular el comportamiento de los diferentes procesos de la cadena ante la incertidumbre y variabilidad de la demanda del consumidor. Se utiliza un modelo de cadena de suministro presente en la literatura académica que introduce la logística inversa y analiza el impacto de la incertidumbre en el reciclaje para una posterior utilización en la producc...

  19. Consecuencias del efecto Bullwhip según distintas estrategias de gestión de la cadena de suministro: modelado y simulación

    OpenAIRE

    Campuzano Bolarín, Francisco; Lario Esteban, Francisco Cruz; Ros Mcdonnell, Lorenzo Brian

    2008-01-01

    [ESP] El efecto Bullwhip es uno de los principales causantes de las inestabilidades en el proceso de gestión de demanda que se producen a lo largo de la cadena de suministro. El presente artículo expone un modelo capaz de recrear diferentes escenarios para la gestión de demanda en una cadena de sumi- nistro determinada, con independencia del número de niveles definidos en la cadena de suministro considerada. El modelo, realizado utilizando la metodología de la dinámica de si...

  20. Turismo sostenible, cadena de valor y participación comunitaria en Suesca (Cundinamarca, Colombia

    Directory of Open Access Journals (Sweden)

    Édgar Moreno

    2011-11-01

    Full Text Available Cadena de Valor y la participación comunitaria, desde las teorías de desarrollo como expansión de libertades y a escala humana, en el proyecto “Fortalecimiento del Turismo Sostenible en Suesca, Cundinamarca”. Se llevó a cabo un análisis a partir de cuatro criterios y cinco indicadores derivados de las teorías mencionadas. Este caso se basó en la transferencia de un modelo de desarrollo ajustado que logró contribuir a la participación comunitaria para el mejoramiento del turismo local. Se identificaron puntos sensibles para hacer de este un proceso replicable.

  1. ELISA for the serology of FIP virus.

    NARCIS (Netherlands)

    A.D.M.E. Osterhaus (Ab); A. Kroon; R.M.S. Wirahadiredja

    1979-01-01

    textabstractAn enzyme linked immunosorbent assay (ELISA) for feline infectious peritonitis (FIP) virus serology is described. The assay is analogous to a previously developed indirect heterologous immunofluorescence test (IFT) in which transmissible gastroenteritis (TGE) viral antigen was used. Comp

  2. Hyperglucagonaemia analysed by glucagon sandwich ELISA

    DEFF Research Database (Denmark)

    Albrechtsen, Nicolai Jacob Wewer; Hartmann, Bolette; Veedfald, Simon;

    2014-01-01

    the extent to which the hyperglucagonaemia measured in clinical samples was caused by authentic glucagon. METHODS: We examined the performance of three commercial glucagon 'sandwich' ELISAs. The ELISA with the best overall performance was selected to compare glucagon measurements in clinical samples...... sensitivity for glucagon in plasma (>10-20 pmol/l). Thus, only the third assay was suitable for measuring glucagon concentrations in clinical samples. The ELISA and RIA measured similar glucagon levels in healthy individuals. Measurements of samples from individuals with abnormally high (type 2 diabetes...... or obese) or very elevated (post vagotomy with pyloroplasty, post-RYGB) glucagon levels were also similar in both assays. However, glucagon levels in participants with ESRD were much lower when measured by ELISA than by RIA, indicating that the apparent hyperglucagonaemia is not caused by fully processed...

  3. Establishment of swine interleukin-6 sandwich ELISA.

    Science.gov (United States)

    Nuntaprasert, A; Mori, Y; Tsukiyama-Kohara, K; Kai, C

    2005-03-01

    We established a sandwich enzyme-linked immunosorbent assay (ELISA) for swine interleukin-6 (SwIL-6), which was applied for detection of SwIL-6 in vitro and in vivo. Anti-SwIL-6 rabbit- and goat-polyclonal antibodies, and monoclonal antibody (mAb) were prepared, conforming that all of the antibodies were reactive with recombinant SwIL-6 by Western blotting and indirect ELISA. A sandwich ELISA was developed using the mAb as a capture antibody and biotinylated goat-polyclonal antibody as a detection antibody. The detection limit of the sandwich ELISA for rSwIL-6 was 49pg/ml and did not show cross-reactivity with swine IL-1b, IL-4, IL-8, IL-18, IL-12, and IFN-g. Using the ELISA, SwIL-6 was detected in culture medium of the monocytes stimulated with PHA-P and PMA, and the plasma or the bronchoalveolar lavage fluid (BALF) of pigs experimentally infected with Actinobacillus pleuropneumoniae or Mycoplasma hyopneumoniae. This ELISA for SwIL-6 may be useful for understanding the role of this cytokine in various swine diseases. PMID:15582688

  4. Field trial of brucellosis competitive ELISA

    International Nuclear Information System (INIS)

    2990 sera samples from cattle were tested for antibodies to Brucella abortus using 8 serological tests for. The tests used were Rose Bengal (RBT), Buffer Plate Agglutination Test (BPAT), Complement Fixation (CFT), 2 Indirect and 2 Competitive Enzyme Linked Immunosorbent Assays (ELISA). Bacteriological evaluation from milk was done also. All tests were compared with respect to diagnostic specificity in vaccinated herds which were considered to be Brucella-free. The diagnostic specificity of the Indirect and Competitive ELISA was greater than 99,8%. Estimates of relative sensitivity were obtained from infected herds. The diagnostic sensitivity of the Indirect ELISA was greater than 95,8% and for the Competitive ELISA between 98,8 and 100 %, the last value refers to the Competitive ELISA Prototype II (SLPS antigen/M84 Mab), which was found highly suitable to differentiate vaccinated from brucella-infected cattle. The use of C-ELISA II for monitoring bovine populations under an eradication programme is recommended. (author)

  5. An overview on ELISA techniques for FMD

    Directory of Open Access Journals (Sweden)

    Ding Yao-zhong

    2011-09-01

    Full Text Available Abstract Background FMD is one of the major causes of economic loss of cloven-hoofed animals in the world today. The assessment of dominant genotype/lineage and prevalent trends and confirmation the presence of infection or vaccination not only provides scientific basis and first-hand information for appropriate control measure but also for disease eradication and regaining FMD free status following an outbreak. Although different biological and serological approaches are still applied to study this disease, ELISA test based on the distinct format, antigen type and specific antibody reinforce its predominance in different research areas of FMD, and this may replace the traditional methods in the near future. This review gives comprehensive insight on ELISA currently available for typing, antigenic analysis, vaccination status differentiation and surveillance vaccine purity and content at all stages of manufacture in FMDV. Besides, some viewpoint about the recent advances and trends of ELISA reagent for FMD are described here. Methods More than 100 studies regarding ELISA method available for FMD diagnosis, antigenic analysis and monitor were thoroughly reviewed. We investigated previous sagacious results of these tests on their sensitivity, specificity. Results We found that in all ELISA formats for FMD, antibody-trapping and competitive ELISAs have high specificity and RT-PCR (oligoprobing ELISA has extra sensitivity. A panel of monoclonal antibodies to different sites or monoclonal antibody in combination of antiserum is the most suitable combination of antibodies in ELISA for FMD. Even though from its beginning, 3ABC is proven to be best performance in many studies, no single NSP can differentiate infected from vaccinated animals with complete confidence. Meanwhile, recombinant antigens and peptide derived from FMDV NPs, and NSPs have been developed for use as an alternative to the inactivated virus antigen for security. Conclusions There is

  6. Construyendo Cadenas de Valor Incluyentes: una comparación de dos casos de biocomercio en Suramérica

    NARCIS (Netherlands)

    Muradian, R.; Verschoor, G.M.; Bolívar, E.; Ochoa, G.

    2012-01-01

    Este artículo analiza la configuración de redes de innovación en dos casos de cadenas de valor de biocomercio en Suramérica: producción de salsas de ajíes con frutas amazónicas en Colombia y producción de perfumes en Brasil a partir de aceites esenciales provenientes de Ecuador. Por medio de la inte

  7. Las Etapas de La Gestión del Conocimiento: Perspectivas relacionadas a las cadenas hoteleras

    Directory of Open Access Journals (Sweden)

    Carolina Sass de Haro

    2014-04-01

    Full Text Available Las Etapas de La Gestión del Conocimiento: Perspectivas relacionadas a las cadenas hoteleras - La gestión del conocimiento es una práctica organizativa que produce beneficios para la toma de decisiones. En un mercado cada vez más competitivo como la hotelería, trabajar efectivamente con la información y el conocimiento es importante. A partir de esto, el objetivo de este trabajo es analizar la aplicación de las cuatro etapas de la gestión del conocimiento (creación, transmisión, almacenamiento y aplicación en las cadenas hoteleras. Se trata de una investigación exploratoria con base en la bibliografía al respecto. Los resultados indican que la aplicación en las cadenas hoteleras de las cuatro etapas de la gestión del conocimiento es más que una tendencia, la gestión del conocimiento en hoteles se hace necesaria en un mercado cada vez más dinámico y globalizado.As Etapas da Gestão do Conhecimento: Perspectivas relacionadas a cadeias hoteleiras - A gestão do conhecimento é uma prática organizacional que produz benefícios para a tomada de decisões. Em um mercado cada vez mais competitivo, como o hoteleiro, trabalhar efetivamente com a informação e o conhecimento, é importante. A partir disto, o objetivo deste trabalho é o de analisar a aplicação das quatro etapas da gestão do conhecimento (criação, transmissão, armazenamento e aplicação nas cadeias hoteleiras. Trata-se de uma pesquisa exploratória e bibliográfica. Os resultados indicam que a aplicação das quatro etapas da gestão do conhecimento às cadeias hoteleiras é mais que uma tendência. A gestão do conhecimento em meios de hospedagem se faz necessária em um mercado cada vez mais dinâmico e globalizado.The Stages of Knowledge Management: perspectives related to the hotel chains - The knowledge management is an organizational practice that produces benefits for decision making. In an increasingly competitive market like the hotel industry, is

  8. La Cadena SER en Facebook: nuevas estrategias de interacción con la audiencia

    Directory of Open Access Journals (Sweden)

    Ramos del Cano, Fátima

    2013-06-01

    Full Text Available En español: El actual contexto periodístico ha consolidado a las redes sociales como nuevas y determinantes herramientas de comunicación e interacción con los usuarios. En este sentido, este artículo tiene por objeto analizar de qué manera la tradicional participación de la audiencia en el medio radiofónico se ha visto afectada por su implantación, centrando nuestro objeto de estudio en el perfil de Facebook (la red de mayor audiencia en España y una de las más significativas a nivel internacional de los programas informativos de la Cadena SER: "A Vivir que son dos días", "Hora 25", "Hoy por Hoy" y "La Ventana". Partiendo de la hipótesis de su infrautilización y aplicando el análisis de contenido, se ha estudiado la actividad de estos programas en sus cuentas de Facebook. Los resultados certifican cómo la emisora no potencia la interacción con los oyentes a través de sus redes sociales. La audiencia, sin embargo, sí que emplea estas plataformas para relacionarse entre sí. La llegada de este medio social, por tanto, ha favorecido y promocionado dinámicas de participación que, a diferencia de las tradicionales, no son impulsadas, ni canalizadas por el medio radiofónico sino por los propios oyentes. In english: The current news media context has consolidated social networks services as the new and decisive communication and interaction tools with users. In this regard, the purpose of this article is to analyze how the traditional audience participation has been affected by its implantation, focusing our inquiry on Facebook profile (being Facebook the network with the greatest audience in Spain and one of the most significant at the international level of Cadena Ser news programmes: "A Vivir que son dos días", "Hora 25", "Hoy por Hoy" and "La Ventana". Taking as the starting-point the hypothesis that it is being underutilized and applying the content analysis, it has been studied the activity of these programmes in regard to

  9. La cadena de suministro del sector metalúrgico: un análisis de sus principales eslabones (The Supply Chain in the metallurgical sector: an analysis of the main links)

    OpenAIRE

    Patricia Carbajo-de-Lera; Rafaela Alfalla-Luque; Carmen Medina-Lopez

    2011-01-01

    Los directivos actuales conciben que la competencia ya no es empresa frente a empresa, sino que desborda este plano generándose una competencia cadena de suministro frente a cadena de suministro. Por ello, la gestión de la cadena de suministro se convierte en un elemento clave para la supervivencia empresarial y la obtención de ventajas competitivas. El diseño de la cadena de suministro viene definido, en buena parte, por las características del sector al que pertenece. En consecuencia, es de...

  10. Cadenas Globales de Valor y Desarrollo: Perspectivas Críticas desde el Sur Global

    Directory of Open Access Journals (Sweden)

    Víctor Ramiro Fernández

    2015-06-01

    Full Text Available Este artículo realiza una interpretación teórica crítica de las cadenas globales de valor, entendidas como una relevante herramienta para el análisis de los procesos de globalización, no sólo a nivel académico sino a nivel institucional, a partir de la formulación de estrategias de desarrollo promovidas por gobiernos y organismos internacionales en el Sur Global. Teniendo en cuenta la evolución del concepto a partir de una perspectiva comparada con la teoría del sistema-mundo que le diera origen, se destacan las debilidades fundadas tanto en aspectos ligados al distanciamiento respecto de dicha teoría, como en los limitantes que surgen producto de la plataforma teórica que ambas comparten. Las restricciones para comprender la inalterada recreación de la estructura jerárquica y desigualadora del capitalismo, y los divergentes escenarios al interior del Sur Global, emergen como los limitantes teórico-empíricos más importantes. En este sentido, el trabajo concluye subrayando algunos desafíos orientados a superarlos.

  11. Modelo de benchmarking de la cadena de abastecimiento para pymes manufactureras.

    Directory of Open Access Journals (Sweden)

    Alfredo Beltrán Amador

    2002-01-01

    Full Text Available El modelo de benchmarking de la cadena de abastecimiento para Pymes manufactureras representa una guía para el mejoramiento de los procesos logísticos de una Pyme dedicada a la producción. En el presente artículo se muestra tanto el desarrollo del modelo como los principales resultados de su implementación en una muestra de empresas del Prodes (Programa de Desarrollo Sectorial de alimentos de Acopi, de la ciudad de Cali. Con base en los resultados de este estudio de aplicación demostrativa y de prueba, se establecen pautas para futuras investigaciones y aplicaciones del modelo en otros sectores. Este modelo ha sido desarrollado con base en los hallazgos del estudio titulado «Propuesta para el desarrollo de un sistema logístico integral para Pymes», realizado por los autores, como opción de grado para el título de Maestría en Administración, en convenio con la Universidad de Tulane.

  12. Radiolinja, Vodafone või Elisa

    Index Scriptorium Estoniae

    2004-01-01

    Jüri Kaljundi, Tarvo Ulejev ning Anne Mere vastavad küsimusele, kas Radiolinja peaks jätma alles olemasoleva nime, võtma nimeks Soomes peagi Radiolinjat asendama hakkava Elisa või rahvusvaheliselt tuntud kaubamärgi Vodafone

  13. Elisa Studio showroom = Elisa Studio showroom / Jan Joonas Graps ; kommenteerinud Tiina Kuusisto

    Index Scriptorium Estoniae

    Graps, Jan Joonas, 1972-

    2015-01-01

    Elisa Studio showroom Helsingis Lasiplatsi, Mannerheimintie 22-24. Sisekujunduse autorid Jan Joonas Graps, Ken-Kristjan Ruut, Anne Määrmann (JanKen Wisespace). Lühidalt loovstuudiost JanKen Wisespace

  14. Las cadenas hoteleras en el mundo y evolución de su operación en México al inicio del siglo XXI

    Directory of Open Access Journals (Sweden)

    Alfonso de Jesús Jiménez Martínez

    2008-12-01

    Full Text Available Las cadenas hoteleras internacionales desempeñan un papel relevante en el sistema turístico internacional, y cada vez hay un mayor número de cuartos asociados a cadenas hoteleras en el mundo. Las más grandes, son empresas gigantescas que actúan a nivel global y con estrategias de escala planetaria. Las cadenas tienen particularidades específicas ligadas a la condición preponderante de los países desarrollados en la economía internacional, y su presencia mundial y regional está ligada a diversos factores que inciden en su funcionamiento. Actualmente, la dinámica de las grandes corporaciones se entiende más por la rentabilidad que promete a los accionistas que por la búsqueda de satisfacción del consumidor (el turista. En este texto se busca una aproximación a la identidad y la distribución regional de las cadenas en la oferta turística mundial y sus particularidades al inicio del siglo XXI. Se describe y analiza la evolución de esas cadenas en México de acuerdo con criterios de categoría y localización.

  15. Utilidad de sangre almacenada en papel de filtro para estudios serologicos por ELISA de inhibicion Use of filter paper strips on an ELISA inhibition test for serologic studies on dengue

    OpenAIRE

    Vázquez, S.; Fernández, R.; C. LLorente

    1991-01-01

    Para evaluar la utilidad del método de recolección de muestras de sangre en papel de filtro para la detección de anticuerpos anti-dengue mediante un ELISA de Inhibición recientemente desarrollado en nuestro laboratorio, se realizó una toma simultánea de muestras de sangre en papel de filtro y de suero, de donantes de Banco de Sangre. Ambas muestras fueron conservadas a -20ºC y probadas a los 15 dias, 3 y 6 meses respectivamente. A las muestras que resultaron positivos se les amplió el rango d...

  16. LA CADENA GLOBAL DE PRENDAS DE VESTIR EN CENTROAMÉRICA Y LAS POSIBILIDADES DE FORTALECER SU COMPETITIVIDAD

    Directory of Open Access Journals (Sweden)

    Álvaro Martín Parada Gómez

    2003-12-01

    Full Text Available Este artículo tiene el interés de analizar los mecanismos por medio de los cuales las empresas de la Industria de la Confección de Prendas de Vestir (pantalones, camisas, trajes, medias, entre otros de Centroamérica se han insertado en la economía internacional. Para estos efectos, se ha considerado la Cadena Global de Mercancías, la cual permite clarificar el desarrollo de la producción de las prendas de vestir desde la adquisición de las materias primas, pasando por el proceso productivo, avanzando hacia el marketing y finalizando en el consumo final.El principal resultado de la investigación permite indicar que las empresas concentran sus esfuerzos únicamente en la fase del proceso productivo, es decir, realizan la labor de ensamblaje de la prenda de vestir, dependiendo directamente de la importación de materias primas en la fase inicial de la cadena y de la direccionalidad del marketing en la tercera fase, ésta última, fundamentalmente es realizada en países desarrollados. El segundo resultado, evidencia que los países de Centroamérica son atractivos para las firmas multinacionales productoras de prendas de vestir, debido a la existencia de bajos costos en la mano de obra, aprovechamiento de los beneficios otorgados por los Regímenes de Zona Francas y por las condiciones pre-establecidas en los acuerdos comerciales como la Iniciativa de la Cuenca del Caribe y el Acuerdo Multifibras.El análisis de la cadena ha permitido observar la posición competitiva de Centroamérica en la economía internacional desde la perspectiva de la industria de la confección de prendas de vestir, así como los retos que deben enfrentarse para insertarse exitosamente en la economía global.

  17. Modelamiento, con dinámica de sistemas, de una cadena de distribución de alimentos refrigerados

    OpenAIRE

    Montoya Moreno, Carlos Andrés

    2011-01-01

    En el capítulo 2, se presenta los conceptos de control y medición, como procesos que permiten lograr una buena gestión y que constituyen una importante base en la construcción de indicadores; posteriormente se desarrollara los conceptos principales de indicadores de gestión, indicadores de gestión logística e indicadores en cadenas de distribución; luego se describe un sistema de indicadores de gestión aplicado a la microempresa Lácteos y Cárnicos “MERCARDY”, finalmente se explica el funciona...

  18. Más por menos: El trabajo precario de las mujeres en las cadenas de producción globalizadas

    OpenAIRE

    Raworth, Kate; Harvey, Claire

    2004-01-01

    La globalización ha introducido a millones de mujeres de los países en desarrollo en el mundo del trabajo. Trabajan en las cadenas globales de producción de las empresas multinacionales, produciendo mercancías que impulsan el crecimiento de las exportaciones: recogen y envasan fruta, cosen prendas de vestir, cortan flores y montan juguetes. Estos trabajos podrían aportar a las mujeres los ingresos, la seguridad y el apoyo que necesitan para salir de la pobreza junto con sus familias. Por el ...

  19. Caracterización de la cadena de suministros de la miel en el estado de Tabasco.

    OpenAIRE

    Pascual González, Josué

    2012-01-01

    El presente trabajo presenta el resultado de la Caracterización de la Cadena de Suministros de la Miel (CSM) en el Estado de Tabasco y la Evaluación de Sistemas de Trazabilidad para la Miel. La CSM está integrada por una serie relaciones ascendentes y descendentes entre unidades de producción, para una fácil compresión en este trabajo, a las unidades de producción se les ha denominado actores de CSM. La caracterización de la CSM en el estado de Tabasco se realizó entre proveedores de mater...

  20. Construyendo Cadenas de Valor Incluyentes: una comparación de dos casos de biocomercio en Suramérica

    OpenAIRE

    Muradian, R.; Verschoor, G.M.; Bolívar, E.; Ochoa, G.

    2012-01-01

    Este artículo analiza la configuración de redes de innovación en dos casos de cadenas de valor de biocomercio en Suramérica: producción de salsas de ajíes con frutas amazónicas en Colombia y producción de perfumes en Brasil a partir de aceites esenciales provenientes de Ecuador. Por medio de la integración de dos perspectivas teóricas, los autores resaltan tanto los aspectos económicos como socioculturales que influencian la configuración de estas redes. Esto permite considerar las tensiones,...

  1. Lean production y gestión de la cadena de suministro en la industria aeronáutica

    OpenAIRE

    Moyano Fuentes, José; Martínez Jurado, Pedro José

    2011-01-01

    En este trabajo se evalúa el estado actual de la investigación sobre Lean Production y Gestión de la Cadena de Suministro en la industria aeronáutica con dos objetivos básicos: 1) identificar el conjunto de líneas de investigación desarrolladas, aportando un criterio de clasificación de la literatura, 2) discutir sobre la evidencia empírica, intentando proporcionar orientaciones para la investigación futura. Para el logro de estos objetivos se ha empleado una metodología de investigación cons...

  2. Determinació del número de ramificacions segons % d’extensor de cadena en PLA

    OpenAIRE

    Cervera de la Varga, Raul

    2015-01-01

    L’àcid polilàctic (PLA) és un polímer biodegradable que està convertint-se en una alternativa als combustibles fòssils. No obstant, presenta males prestacions a altes temperatures. Des de l’UPC en col·laboració amb el Centre Català del Plàstic (CCP) s’està fent un gran estudi afegint al PLA extensors de cadena per tal de superar aquestes limitacions. Es disposa de mostres ramificades de PLA amb diferents graus d’extensor. És necessari un procediment per obtenir informació sobre...

  3. Modelo estocástico para planificar cadenas de suministro con productos de ciclos de vida cortos

    Directory of Open Access Journals (Sweden)

    Jairo Coronado-Hernandez

    2010-07-01

    Full Text Available En este trabajo se presenta un modelo de optimización estocástica para la planificación de cadenas de suministros para productos con ciclo de vida corto, a través de la decisión de reservar la capacidad a contratar en los recursos de los proveedores antes de tener certeza del comportamiento de la demanda. Se consideran recursos alternativos, múltiples productos con lista de materiales complejas, demanda distribuida a lo largo de periodos consecutivos, ciclos de vida cortos, lead time largos y altos niveles de incertidumbre representados en forma de escenarios.

  4. Manejo estratégico de la calidad ambiental en las cadenas agroalimentarias. Aplicaciones a la arveja china guatemalteca

    OpenAIRE

    Jiménez Porras, Gerardo; Pelupessy, Wim

    2006-01-01

    Desde la perspectiva de las cadenas globales de mercancías, considerando la secuencia de actividades insumo-producto (principalmente producción primaria, procesamiento, comercialización y uso final), este artículo analiza las interacciones (horizontales) entre productores agrícolas como un juego del tipo dilema del prisionero. El resultado esperado es un equilibrio Nash en el cual los productores se quedan atados al uso de tecnologías no sostenibles. Sin embargo, la consideración de los movim...

  5. Reflexiones sobre la propuesta de reforma constitucional en Colombia para la introducción de la cadena perpetua:

    Directory of Open Access Journals (Sweden)

    Lina Mariola Díaz Cortés

    2009-06-01

    Full Text Available El artículo analiza los diferentes proyectos de ley que buscan dar respuesta a episodios esporádicos de violencia sexual contra menores. De esta forma se estudia la política criminal “irracional” que se ha propuesto en Colombia para los delitos sexuales contra menores, es decir, en concreto, las propuestas absurdas e injustificadas de implementar instrumentos como la cadena perpetua, que desconocen principios básicos contenidos dentro de nuestra Carta de derechos y que han sido acogidos desde antaño por penalistas que defienden la utilización racional de los instrumentos punitivos del Estado.

  6. Anatomía de la cadena de prestación de salud en Colombia en el régimen contributivo

    Directory of Open Access Journals (Sweden)

    Yuri Gorbaneff

    2004-01-01

    Full Text Available El sistema de prestación de salud en el régimen contributivo en Bogotá se analiza como una cadena productiva de prestación de servicios. Se identifican los miembros primarios y secundarios de la cadena, su estructura vertical y horizontal, así como los procesos transversales que en ella se desarrollan. La descripción de la cadena permite identificar los altos costos de transacción como el principal problema que la aqueja. Éstos dificultan el funcionamiento del mecanismo de precios, hacen imposible la competencia perfecta e imponen formas de organización híbridas. En este artículo se plantean temas para futuras investigaciones, con miras a optimizar las formas de gobernación de las transacciones, abaratar los costos del sistema y liberar recursos para el régimen subsidiado.

  7. SISTEMAS DE IDENTIFICACIÓN POR RADIOFRECUENCIA, CÓDIGO DE BARRAS Y SU RELACIÓN CON LA GESTIÓN DE LA CADENA DE SUMINISTRO

    Directory of Open Access Journals (Sweden)

    ALEXANDER CORREA ESPINAL

    2010-01-01

    Full Text Available El articulo muestra, a través del análisis de libros, artículos científicos y algunos estudios existentes, el estado del arte y la relación entre los sistemas e identificación (código de barras y radiofrecuencia y la gestión de la cadena de suministro, en aplicaciones significativas a nivel mundial y de Colombia. Para ello, se compilan conceptos generales de los sistemas de identificación presentando sus definiciones, componentes de hardware/software, aplicaciones en la cadena de suministro y el uso que grandes, medianas y pequeñas empresas le otorgan. El estudio pone en evidencia la importancia que estas tecnologías tienen para mejorar el desempeño y la trazabilidad, simplificar operaciones, reducir costos y agilizar los flujos de información en la cadena de suministro.

  8. La influencia de la integración de la cadena de suministro en la implantanción de capacidades de comercio electrónico

    Directory of Open Access Journals (Sweden)

    José Moyano-Fuentes

    2010-01-01

    Full Text Available Este trabajo analiza la influencia de la integración de la cadena de suministro en la implantación de capacidades de comercio electrónico. Para ello, se parte de que la relación entre implantación de TICs y eficiencia está determinada por el uso de estas tecnologías y que existe una correlación positiva entre la vinculación de la estrategia de la cadena de suministro con la estrategia empresarial y la adopción de comercio electrónico. Los resultados obtenidos en la industria del automóvil señalan que el nivel de integración de los flujos físicos en la cadena de suministro influye significativamente en el nivel de implantación de capacidades de comercio electrónico.

  9. Modelización de una cadena de abastecimiento (supply chain) para el sector textil-confección en el entorno colombiano

    OpenAIRE

    Ramírez Echeverri, Sergio Augusto

    2010-01-01

    Este trabajo modela la cadena de abastecimiento de la empresa Creaciones Nadar S.A, en la ciudad de Medellín y se utiliza la dinámica de sistemas. La empresa se dedica a la confección, distribución y comercialización de los productos de la marca Speedo® en todo el país y para el exterior. Se realizan entrevistas y encuesta con expertos en la cadena textil confección. Con la información se hace un modelo de la cadena de suministro, con dinámica de sistemas, y con la utilización del software It...

  10. Las capacidades de absorción del conocimiento como ventajas competitivas para la inserción de pymes en cadenas globales de valor

    Directory of Open Access Journals (Sweden)

    Jaime Olea-Miranda

    2016-01-01

    Full Text Available El propósito del estudio es identificar las capacidades de absorción del conocimiento como fuente de ven-tajas competitivas que potencializan en las pymes las posibilidades de integrarse a las cadenas globalesde valor. Para ello se realizó una revisión del estado del arte que fundamenta la propuesta de un modelobasado en 2 enfoques teóricos: el de sistemas de innovación y el de cadenas globales de valor. La imple-mentación se realizó en pymes del noroeste de México en los sectores de tecnologías de informacióny metalmecánica. Los resultados muestran que cuando las pymes locales son proveedoras de empresastransnacionales reciben transferencia de conocimientos que les permiten ingresar o mejorar su posiciónen las cadenas globales de valor.

  11. Sero-epidemiology of Breda virus in cattle using ELISA

    NARCIS (Netherlands)

    Horzinek, M.C.; Koopmans, M.P.; Boom, U. van den; Woode, G.N.

    1989-01-01

    Two direct blocking enzyme linked immunosorbent assays (ELISA) for the detection of antibodies to Breda virus in sera of cattle were compared. An ELISA with consecutive addition of antigen and test serum to an antibody-coated plate gave higher positive: negative absorbence ratios than an ELISA in wh

  12. El Efecto Bullwhip en las Cadenas de Suministro: Una Aproximación Basada en Técnicas de Inteligencia Artificial

    OpenAIRE

    Ponte Blanco, Borja

    2013-01-01

    Varias son las circunstancias que, en las dos últimas décadas, han concedido a la gestión de la cadena de suministro un papel estratégico en la búsqueda de ventajas competitivas. Así, tras una exhaustiva revisión bibliográfica sobre la materia abordada, este documento aplica la metodología multiagente para optimizar dicha gestión. Para ello, representa la cadena de suministro como un Sistema Global Multiagente, compuesto a su vez por cuatro Subsistemas Multiagente, que replican el comportamie...

  13. Seguridad en la cadena de suministro basada en la norma iso 28001 para el sector carbón, como estrategia para su competitividad

    OpenAIRE

    CORREA ESPINAL, ALEXANDER; GÓMEZ MONTOYA, RODRIGO ANDRÉS

    2011-01-01

    El presente artículo busca revisar y analizar el aporte e impacto de la cadena de suministro y su seguridad, basándose en la adopción de la norma internacional ISO 28001:2007, como estrategia para mejorar la competitividad y vinculación a mercados internacionales del carbón colombiano. Para alcanzar este objetivo, se revisan libros, informes y artículos, que cubren desde la descripción del sector minero en Colombia, pasando por conceptos de cadena de suministro y su estructura en el sector...

  14. PROPUESTA DE UN MODELO DE INTEGRACIÓN PARA LA GESTIÓN DE LA CADENA DE SUMINISTRO EN EL SECTOR DE LA CONSTRUCCIÓN

    OpenAIRE

    CARBONELL UREÑA, JUAN FRANCISCO

    2012-01-01

    Hoy en día, y en nuestro entorno, el sector de la construcción está experimentando grandes cambios en todos los aspectos relacionados con su negocio: el mercado, las relaciones entre competidores, los mecanismos de financiación, los recursos de mano de obra, etc. es por ello que en esta Tesis se pretende analizar modelos existentes de integración de la Cadena de Suministro que recojan una manera de enfocar los problemas a los que se tiene que enfrentar este sector y las cadenas de producción ...

  15. SISTEMAS DE IDENTIFICACIÓN POR RADIOFRECUENCIA, CÓDIGO DE BARRAS Y SU RELACIÓN CON LA GESTIÓN DE LA CADENA DE SUMINISTRO

    OpenAIRE

    ALEXANDER CORREA ESPINAL; CARLOS ESTEBAN ÁLVAREZ LÓPEZ; RODRIGO ANDRÉS GÓMEZ MONTOYA

    2010-01-01

    El articulo muestra, a través del análisis de libros, artículos científicos y algunos estudios existentes, el estado del arte y la relación entre los sistemas e identificación (código de barras y radiofrecuencia) y la gestión de la cadena de suministro, en aplicaciones significativas a nivel mundial y de Colombia. Para ello, se compilan conceptos generales de los sistemas de identificación presentando sus definiciones, componentes de hardware/software, aplicaciones en la cadena de suministro ...

  16. Conocimiento y aprendizaje en las grandes cadenas hoteleras españolas en Iberoamérica. Internet como herramienta de aprendizaje organizativo

    OpenAIRE

    Rodríguez Antón, José Miguel; Alonso Almeida, Mar; Rubio Andrada, Luis; Esteban Alberdi, Cristina

    2008-01-01

    El aprendizaje y la dirección del conocimiento organizativo se han convertido en sendos elementos clave para la dirección de las cadenas hoteleras. El hecho de que múltiples tipos de clientes se alojen en sus hoteles dificulta un trato homogéneo y estandarizado que sea capaz de atender las preferencias específicas de cada cliente haciéndole sentir un huésped especial. Por este motivo, va a ser necesario conjugar una política general para toda la cadena con un trato personalizad...

  17. Análisis de la cadena de valor del queso manchego en Cuenca, España Analisys of the manchego cheese value chain in Cuenca, Spain

    OpenAIRE

    Brenda Inoscencia Trejo Téllez; Ignacio de los Ríos Carmenado; Benjamín Figueroa Sandoval; Francisco José Gallego Moreno; Francisco Javier Morales Flores

    2011-01-01

    El concepto de cadena de valor, se ha desarrollado como respuesta a la necesidad para que las empresas rurales, cumplan con las exigencias de la demanda de productos especializados de alta calidad. En este trabajo se realiza un análisis de la cadena de valor del queso manchego en Cuenca, España; identificando a los agentes y las relaciones entre ellos; en donde se tiene una importante producción de queso manchego con denominación de origen. La investigación se llevó a cabo durante 2008 en la ...

  18. Relación entre los niveles de flexibilidad de la cadena muscular posterior y lesiones músculo tendinosas en jugadores de rugby

    OpenAIRE

    Alessio, Germán

    2015-01-01

    La práctica de rugby produce posturas hipertónicas que traen como consecuencia un acortamiento de los músculos de la cadena posterior, lo que lleva aparejado una reducción de la flexibilidad. Objetivo: Establecer la relación entre los niveles de flexibilidad de la cadena muscular posterior con la cantidad de lesiones músculo-tendinosas en jugadores de rugby. Material y Métodos: La investigación es no experimental, transversal y descriptiva. Se evalúo la flexibilidad a través de un cajón...

  19. Caracterización de la cadena de abastecimiento en las pymes de la comunicación gráfica impresa en Colombia

    OpenAIRE

    2012-01-01

    La monografía “Caracterización de la cadena de abastecimiento en las pymes de la comunicación gráfica impresa en Colombia” es una investigación que identifica los componentes y la relación existente con las pymes (pequeñas y medianas empresas) en la cadena de abastecimiento de la comunicación gráfica en Colombia. El objetivo planteado para esta monografía es realizar una propuesta de desarrollo de proveedores en las pymes de la industria de la comunicación gráfica impresa en Colombia, tenie...

  20. Consideraciones sobre las máquinas de neumáticos y cadenas (Parte I

    Directory of Open Access Journals (Sweden)

    Chinchilla, M.

    1966-11-01

    Full Text Available The continued development of machinery takes place usually in a sustained and progressive manner. However, occasionally new possibilities emerge, which open up new fields and require entirely new techniques, both in the use, construction and design of the new equipment. A particular case is the adoption of wheels with pneumatic tyres on bulldozers, as a result of their use in loading units. The changes that have occurred in these last ten years have been so important that it may well be said that until recently the use of pneumatic tyres on bulldozers and loaders had not been clearly defined, in terms of their functions. At present the main characteristics that differentiate machines of the same type using tyres and those using caterpillars can be definitely enunciated. One of the main factors is that machinery using tyres must be limited to very specific tasks, whilst equipment using continuous tracks can carry out bulldozing and loading jobs under highly varied conditions.La evolución de la maquinaria se realiza normalmente de una forma continuada y progresiva. De vez en cuando aparecen, sin embargo, posibilidades nuevas que abren campos diferentes y que requieren técnicas distintas, tanto en el empleo como en la construcción y diseño de estos tipos de maquinaria. Uno de estos casos concretos, lo ha sido la aparición de las ruedas neumáticas en las máquinas topadoras, como consecuencia de su empleo en las unidades cargadoras. Los cambios sufridos en estos últimos diez años han sido tan grandes, que puede decirse que hasta hace muy poco el empleo de los neumáticos en las máquinas topadoras y cargadoras no ha quedado bien delimitado en cuanto a sus requerimientos y sus posibilidades. De todas formas, ya pueden enunciarse las principales características que diferencian las máquinas de cadenas y neumáticos, de la misma clase; una de estas características es la particularización que requieren las máquinas de neumáticos en su

  1. LEAN PRODUCTION Y GESTIÓN DE LA CADENA DE SUMINISTRO EN LA INDUSTRIA AERONÁUTICA

    Directory of Open Access Journals (Sweden)

    Moyano Fuentes, José

    2011-01-01

    Full Text Available En este trabajo se evalúa el estado actual de la investigación sobre Lean Production y Gestión de la Cadena de Suministro en la industria aeronáutica con dos objetivos básicos: 1 identificar el conjunto de líneas de investigación desarrolladas, aportando un criterio de clasificación de la literatura, 2 discutir sobre la evidencia empírica, intentando proporcionar orientaciones para la investigación futura. Para el logro de estos objetivos se ha empleado una metodología de investigación consistente en la identificación y revisión de la literatura. El análisis sistemático posterior de ésta ha sido de naturaleza cualitativa. Este análisis ha permitido identificar tres líneas de investigación en torno a Lean Production y otras tres relacionadas con la Gestión de la Cadena de Suministro. Por último, se identifican nuevas oportunidades y retos a los que se enfrenta la investigación en un futuro.

  2. Evaluación del desempeño ambiental bajo enfoque de cadena de abastecimiento verde

    Directory of Open Access Journals (Sweden)

    William Ariel Sarache-Castro

    2015-01-01

    Full Text Available Entre los enfoques existentes de gestión ambiental, la gestión de cadenas de abastecimiento verde (Green Supply Chain Managemen t, GSCM, se ha convertido en uno de los más relevantes en los últimos años. El en foque GSCM aborda todos los procesos y actividad es que componen la cadena de abastecimiento en los eslabones de aprovi sionamiento, manufactura y entregas, incluyendo también el diseñ o, las compras, el marketing y la logística inve rsa. Aunque la revisión bibliográfica permiti ó identificar la existencia de una gran v ariedad de indicadores de desempeño ambiental que se enmarcan en el enfoque PresiónEstadoRes puesta, no se encontraron indicadores aplica dos bajo un enfoque GSCM. Por tanto, el presente ar tículo expone una metodología para la cons trucción de un indicador de corte multicrit erio que permite evaluar el desempeño ambiental desde el enfoque GSCM, con base en 8 procesos y 32 actividades detectadas en la revisión de literatura. El indicador fue aplicado en un grupo de 14 empresas de la región cafetera de Colombia. Los resultados indican un d esempeño heterogéneo entre las compañías analizadas y un rezago importante en algunos procesos.

  3. Development of ELISA for diagnosis of allergy to Dermatophagoides farinae.

    Science.gov (United States)

    Ho, T M; Radha, K; Shahnaz, M; Singaram, S P

    1993-09-01

    An indirect enzyme linked immunosorbent assay (ELISA) was developed for the diagnosis of allergy to a house dust mite, Dermatophagoides farinae. The efficacy of the ELISA was then evaluated against a prick test using a commercial allergen. Eighty five suspected allergic rhinitis patients from the Otorhinolaryngology Department, Kuala Lumpur General Hospital, were tested with the ELISA and prick test. Prick test and ELISA results were positive in 84.7% and 80.0% of the patients respectively. The ELISA was found to have 87.5% sensitivity, 61.5% specificity, 92.6% positive predictive value, 47.1% negative predictive value, 7.4% false positive and 52.9% false negative. There was total agreement between the prick test and ELISA for prick test grades of 3+ and 4+. It is concluded that the ELISA is a useful assay for detection of individuals who are highly sensitive to D. farinae.

  4. Detection of canine echinococcosis by coproantigen ELISA

    Institute of Scientific and Technical Information of China (English)

    DeS; PanD; BeraAK; SreevatsavaV; DasSK; DasS; RanaT; BandyopadhyayS; BhattacharyaD

    2010-01-01

    Objective:To study the canine echinococcosis by coproantigen ELISA method. Methods:During the present investigation experimental infection was established using evaginated worms of Echinococcus granulosus (E. granulosus). To check cross reactivity two pups were infected with Taenia hydatigena(T. hydatigena). In order to detect the presence of antigen, hyperimmune sera were raised against excretory-secretory products of adult worms E. chinococcus granulosus. Faecal sample collected either from experimentally infected pups or from other sources were heated at 70℃to detect heat stable soluble antigen. Results:Pups harbouring less than 104 worms showed negative results. Samples collected from 14 days onwards from experimentally infected animals harbouring more than 104 worms showed positive value. The maximum positive samples were detected in samples collected from in and around slaughter house and the least number of samples were detected positive maintained by dog squad. Conclusions:The affinity purified IgG exhibited promising results for detection of canine echinococcosis by indirect ELISA.

  5. Análisis de la Cadena de Suministro y su relación con la Logística: caso del Centro Michelin Valladolid

    OpenAIRE

    Villafañe Alonso, Pablo

    2014-01-01

    El Alcance del Proyecto es el estudio y análisis general de los conceptos Cadena de Suministro y Logística y la aplicación en la empresa Michelin y en su Centro de Valladolid. Además, se proporcionan una serie de conclusiones y mejoras aplicables para la empresa.

  6. Relaciones de asociatividad y cooperación de las empresas familiares medianas en la cadena productiva metalmecánica en la ciudad de Bogotá

    OpenAIRE

    2011-01-01

    En esta tesina se describe un marco teórico sobre las relaciones de asociatividad y cooperación entre las empresas para el desarrollo de sus ventajas competitivas, identificando los factores que las determinan, tomando las experiencias y modelos de cluster, cadenas productivas, redes y distritos industriales. Partiendo de las condiciones y necesidades de competitividad de las empresas familiares

  7. Modelo de diseño de nodos de integración en las cadenas de suministro/Design model of integration nodes in the supply chains

    Directory of Open Access Journals (Sweden)

    Yinef Pardillo-Baez

    2013-01-01

    Full Text Available La débil integración entre empresas de las cadenas de suministro, el hecho de que las entidadesno tienen un procedimiento para identificar los parámetros e indicadores de coordinación entreprocesos, la ineficiente gestión integrada de recursos y capacidades y el inadecuado uso de loscontratos como herramienta de coordinación entre los actores de la cadena de suministro; sonelementos que caracterizan la práctica empresarial de las cadenas de suministro cubanas. Elsiguiente trabajo plantea las bases del Modelo de Diseño de los Nodos de Integración en lascadenas de suministro, y sus aplicaciones. El Modelo es una herramienta de diseño, a la vez queapoya la toma de decisiones en los procesos. Se demuestra que al aplicar el modelo y suprocedimiento, la cadena obtiene mejores resultados e incrementa su integración. Comoconclusión fundamental se obtiene que el Modelo es aplicable y brinda resultados positivos para lapráctica empresarial cubana.

  8. Aplicación del benchmarking en la gestión de la cadena de aprovisionamiento sanitaria: efectos sobre el coste y la calidad de las compras

    Directory of Open Access Journals (Sweden)

    David Naranjo-Gil

    2015-03-01

    Conclusión: Con un proceso de benchmarking, los centros sanitarios pueden ahorrar gastos y realizar una gestión más eficiente de la cadena de aprovisionamiento. El benchmarking facilita que los centros sanitarios adquieran productos a precios más reducidos y de mayor calidad.

  9. Acil Coenzima-A sintetasas de cadena larga (ACSLs) como biomarcadores cito-serológicos en enfermedades inflamatorias o autoinmunes

    OpenAIRE

    Matesanz, F; Fedetz, María; Ndagire, D.; Alcina, Antonio; Sabio, José Mario

    2010-01-01

    Uso de las Acil-Coenzima-A sintetasas de cadena larga (ACSL) como biomarcadores cito-serológicos de enfermedades inflamatorias o autoinmunes, y especialmente de lupus eritematoso sistémico, y método de obtención de datos útiles para el diagnóstico y seguimiento de dichas enfermedades. Venta

  10. Mycotoxin determination using RIA and ELISA methods

    International Nuclear Information System (INIS)

    Experience is summed up of various authors with the determination of some mycotoxins (aflatoxin, ochratoxin A, T-2 toxin, rubratoxin, zearalenon, sterigmatocystine) using radioimmunoassay and enzyme immunoassay. For RIA purposes, tritium or sometimes iodine 125 is frequently used for labelling mycotoxins. Mycotoxins do not show immunogenic properties and must thus be conjugated with a high-molecular compound (serum albumin, polylysine) prior to immunozation. Factors are discussed making mycotoxin determination in foods difficult. Specificity of the obtained antisera is total between the individual mycotoxin groups while cross reactions are always recorded within the groups. RIA makes it possible to determine down to 200 pg (labelled with 3H) or 5 pg (labelled with 125I) of mycotoxins in a standard solution. In addition to high sensitivity and specificity, immunoassays of mycotoxins minimize the quantities of samples and solvents needed for extraction. Large series of samples can be processed using automatic analyzers. ELISA generally is more advantageous than RIA. (M.D.)

  11. eLISA and the Gravitational Universe

    Science.gov (United States)

    Danzmann, Karsten

    2015-08-01

    The last century has seen enormous progress in our understanding of the Universe. We know the life cycles of stars, the structure of galaxies, the remnants of the big bang, and have a general understanding of how the Universe evolved. We have come remarkably far using electromagnetic radiation as our tool for observing the Universe. However, gravity is the engine behind many of the processes in the Universe, and much of its action is dark. Opening a gravitational window on the Universe will let us go further than any alternative. Gravity has its own messenger: Gravitational waves, ripples in the fabric of spacetime. They travel essentially undisturbed and let us peer deep into the formation of the first seed black holes, exploring redshifts as large as z ~ 20, prior to the epoch of cosmic re-ionisation. Exquisite and unprecedented measurements of black hole masses and spins will make it possible to trace the history of black holes across all stages of galaxy evolution, and at the same time constrain any deviation from the Kerr metric of General Relativity. eLISA will be the first ever mission to study the entire Universe with gravitational waves. eLISA is an all-sky monitor and will offer a wide view of a dynamic cosmos using gravitational waves as new and unique messengers to unveil The Gravitational Universe. It provides the closest ever view of the early processes at TeV energies, has guaranteed sources in the form of verification binaries in the Milky Way, and can probe the entire Universe, from its smallest scales around singularities and black holes, all the way to cosmological dimensions.

  12. Identificación de conglomerados para impulsar las cadenas productivas de carne en México.

    Directory of Open Access Journals (Sweden)

    Gabriela Rodríguez-Licea

    2016-06-01

    Full Text Available El objetivo del presente trabajo fue realizar un análisis de correlación espacial regional de la producción de carne en canal por especie en México. El estudio se llevó a cabo durante el periodo 2000 -2012. A través del análisis exploratorio de datos, de la estimación del índice de Moran y de la prueba de LISA, se buscó evidencias de dependencia espacial e identificar conglomerados que impulsen la integración de las cadenas productivas. El análisis exploratorio aporta evidencia de que los estados del norte pueden formar conglomerados que impulsen la producción carne de bovino, porcino y pollo; y el incremento en la producción de carne de ovino y caprino, podría darse entre estados de la región centro y del norte, respectivamente. Los índices positivos obtenidos para la carne de bovino, ovino y caprino, mostraron que la vecindad de estados productores no afectaría la conformación de conglomerados para incrementar la producción; mientras que, la autocorrelación negativa para la carne de porcino y pollo, hace suponer que la situación de vecindad de un estado productor se vería afectada por los agrupamientos regionales de sus vecinos. Con la prueba de LISA, se identificaron grupos estadísticamente significativos que soportan la evidencia de que la concentración espacial de la producción favorece la conformación de clúster y la integración de cadenas productivas únicamente para la carne de bovino, ovino y caprino. 

  13. DETERMINATION OF HISTAMINE IN FISH USING ELISA TECHNIQUE

    NARCIS (Netherlands)

    KRUGER, C; SEWING, U; STENGEL, G; KEMA, [No Value; WESTERMANN, J; MANZ, B

    1995-01-01

    The analysis of histamine in fish and fish products via competitive ELISA is described. The advantages of this method are easy sample preparation and handling, screening capabilities, and low costs. Automation enables the performance of the assay with higher series of samples. The Histamine-ELISA is

  14. A rapid method to improve protein detection by indirect ELISA

    Science.gov (United States)

    The enzyme-linked immunosorbant assay (ELISA) is a rapid, high-throughput, quantitative immunoassay for the selective detection of target antigens. The general principle behind an ELISA is antibody mediated capture and detection of an antigen with a measureable substrate. Numerous incarnations of th...

  15. A micro ELISA for the diagnosis of cerebral cisticercosis.

    Science.gov (United States)

    Nunez, R; Munoz, A; Nunez, C; Gomez, B

    1989-01-01

    A micro ELISA for cysticercosis in the central nervous system was evaluated in 24 patients and compared with indirect hemagglutination. CSF ELISA results were positive in 17 of 20 patients (sensitivity 85%), whereas CSF indirect hemagglutination was positive in only 14 patients (sensitivity 70%).

  16. Agricultural production - Phase 2. Indonesia. ELISA for epidemiology of brucellosis

    International Nuclear Information System (INIS)

    This document is a travel report of a three-week mission (from October 13 to November 1, 1991) to Indonesia within the framework of ''The implementation of ELISA technology for the sero-diagnosis of important livestock diseases''. The mission evaluated the implementation of ELISA technology at the Regional Laboratories and its role in the surveillance and control of bovine brucellosis

  17. ANÁLISIS DE VARIABLES ESTRATÉGICAS PARA LA CONFORMACIÓN DE UNA CADENA PRODUCTIVA DE QUINUA EN COLOMBIA

    Directory of Open Access Journals (Sweden)

    Luz Alexandra Montoya Restrepo

    2005-12-01

    Full Text Available En este artículo se presenta una síntesis de la investigación realizada por el Grupo Interdisciplinario Biogestión sobre los usos y la producción de quinua en Colombia. Se ofrece un análisis estratégico del sector quinuero en el país a fin de identificar los factores clave de cambio que tienen un moderado o alto grado de gobernabilidad y que podrían afectar la conformación de la cadena productiva de quinua. También se proponen algunas estrategias orientadas a controlar estos factores de cambio para lograr la adecuada conformación de la cadena.

  18. INNOVACIÓN TECNOLÓGICA DE LA CADENA AGROALIMENTARIA DE MAÍZ PARA MEJORAR SU COMPETITIVIDAD: ESTUDIO DE CASO EN EL ESTADO DE HIDALGO

    OpenAIRE

    Georgel Moctezuma-López; José Antonio Espinosa-García; Venancio Cuevas-Reyes; José Luis Jolalpa-Barrera; Fernando Romero-Santillán; Alejandra Vélez-Izquierdo; Diana Elisa Bustos Contreras

    2010-01-01

    El objetivo de esta investigación fue identificar la oferta y demanda tecnológica para plantear alternativas en la innovación de la cadena agroalimentaria de maíz en el estado de Hidalgo, México. Se realizaron las siguientes etapas: 1) caracterización de la cadena; 2) identificación de los factores críticos; 3) distinción de demandas tecnológicas de conocimiento y política; 4) reconocimiento de la oferta tecnológica y 5) cuantificación de proyectos de investigación y política pública. Se apli...

  19. Modelos para la planificación centralizada de la producción y el transporte en la cadena de suministro: una revisión

    OpenAIRE

    Josefa Mula; David Peidro; Manuel Díaz-Madroñero; JORGE E. HERNÁNDEZ

    2010-01-01

    Este artículo presenta una revisión bibliográfica sobre los modelos de programación matemática para la planificación centralizada de la producción y el transporte en una cadena de suministro. El propósito de esta revisión es identificar la investigación actual y futura en este campo y proponer un marco de clasificación basado en los elementos siguientes: estructura de la cadena de suministro, nivel de decisión, enfoque de modelado, propósito, información intercambiada y aplicación práctica...

  20. La influencia de la integración de la cadena de suministro en la implantanción de capacidades de comercio electrónico

    OpenAIRE

    José Moyano-Fuentes

    2010-01-01

    Este trabajo analiza la influencia de la integración de la cadena de suministro en la implantación de capacidades de comercio electrónico. Para ello, se parte de que la relación entre implantación de TICs y eficiencia está determinada por el uso de estas tecnologías y que existe una correlación positiva entre la vinculación de la estrategia de la cadena de suministro con la estrategia empresarial y la adopción de comercio electrónico. Los resultados obtenidos en la industria del automóvil señ...

  1. El papel de las TIC en el rendimiento de las cadenas de suministro: el caso de las grandes empresas de España y Portugal

    Directory of Open Access Journals (Sweden)

    Nicolás Gonzálvez- Gallego

    2010-01-01

    Full Text Available Este artículo analiza las diferencias existentes entre las grandes empresas de España y Portugal en lo referido tanto al uso de las Tecnologías de la Información y la Comunicación (TIC como a la integración electrónica con los agentes de la cadena de suministro. Estas diferencias se establecen en términos de la capacidad para generar resultados empresariales superiores. Los resultados muestran diferencias entre ambos grupos de empresas y confirman que las empresas españolas deberían perseguir una mayor integración con la cadena de suministro, por ejemplo, a través del desarrollo de TIC que faciliten la colaboración con clientes y proveedores.

  2. Estudio de los intercambiadores de cadenas utilizados en la abricación de cemento por vía húmeda

    Directory of Open Access Journals (Sweden)

    Papadakis, M.

    1964-12-01

    Full Text Available Not availableEl empleo de cadenas como intercambiador de calor es de uso muy extendido por su robustez y reducido gasto de entretenimiento. Su eficacia está comprobada. No obstante, ciertas dificultades que se presentan durante la marcha y, más acentuadas todavía, durante la puesta en servicio de un horno nuevo, demuestran que el problema no está aun completamente resuelto. Estas dificultades son de orden reológico. Este estudio tiene por objeto proporcionar un método que permite calcular la repartición y dimensionado óptimo de las cadenas teniendo en cuenta las propiedades reológicas de la pasta. El aspecto térmico de la cuestión parece, en efecto, mucho menos importante.

  3. USO DE CADENAS DE MARKOV PARA LA PREDICCIÓN DE LA DINÁMICA DEL COMPORTAMIENTO DE PACIENTES EN UNA UNIDAD DE CUIDADO INTENSIVO CARDIOLÓGICA USE OF MARKOV CHAINS IN PREDICTION OF THE DYNAMICS OF PATIENT BEHAVIOR IN A CARDIOLOGIC INTENSIVE CARE UNIT

    Directory of Open Access Journals (Sweden)

    Víctor Albornoz

    2006-08-01

    Full Text Available En este trabajo presentamos un modelo probabilístico que contribuye al estudio de la dinámica en el comportamiento y permanencia de pacientes en una unidad de cuidados intensivos cardiológica. El modelo utilizado corresponde a una Cadena de Markov en tiempo discreto, que mediante la definición de determinados niveles de gravedad de un paciente (estados y la obtención de las correspondientes probabilidades de transición entre un nivel de gravedad y otro, permite predecir los tiempos de permanencia. Los diferentes estados empleados se basan en la construcción de un nuevo score creado para este propósito. Se muestran los detalles de la metodología adoptada y los principales resultados alcanzados en la aplicación del modelo empleado.In this paper we present a probabilistic model that contributes to the study of dynamics in the behavior and permanence of patients in a cardiovascular intensive care unit. The model corresponds to a discrete Markov Chain, that allows to predict the time that a patient remains in the system through the time, by means of certain severity of illness states and the corresponding transition probabilities between those states. The different states are based on the construction of a new score created for this study. We summarize the details of the adopted methodology and the main results reached in the application of the model.

  4. Diagnóstico molecular de Campylobacter en la cadena avícola destinada para consumo humano en Costa Rica.

    Directory of Open Access Journals (Sweden)

    Leana Zumbado-Gutiérrez

    2014-07-01

    Full Text Available El objetivo de este estudio fue determinar la presencia y la frecuencia de Campylobacter jejuni y Campylobacter coli, en la cadena de producción avícola en Costa Rica. Se evaluaron granjas, planta de proceso y puntos de venta del Gran Área Metropolitana. Para la detección y aislamiento de Campylobacter sp. se analizaron 84 muestras provenientes de la cadena avícola (24 muestras de carne de pollo tomadas en punto de venta, 20 enjuagues de carcasa y 40 de ciegos tomados en planta de beneficio, obtenidas en noviembre de 2012, siguiendo el protocolo ISO 10272-1:2006 modificado por el Departamento de Agricultura de Estados Unidos (USDA, por sus siglas en inglés. De las 84 muestras analizadas, 36 (42,8% resultaron positivas para C. jejuni, y una (1,2% para C. coli. La cantidad de muestras positivas según la Reacción en Cadena de Polimerasa (PCR, por tipo de muestra fue: para contenido cecal, 16 (n=40, para carcasas, 8 (n=20 y para punto de venta 12 (n=24. Dieciséis de las veinte granjas muestreadas fueron positivas. Hubo una alta frecuencia de Campylobacter en todos los puntos de la cadena muestreados, lo que podría estar asociado a modificaciones de parámetros relacionados con inocuidad en cada eslabón. El enfriamiento rápido y el uso de cloro en el agua en la planta de proceso, disminuyó la frecuencia de muestras positivas de Campylobacter spp.

  5. SRM, motor para el desarrollo de la cadena de valor Supplier Relationship Management – SRM gestión de las relaciones con los proveedores

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    Luis Leonardo Mendoza S.

    2010-01-01

    Ejemplo de ello es el SRM, Supplier Relationship Management, en donde por medio de la constitución de acuerdos basados en intereses comunes, se establecen relaciones estrechas, funcionales y eficientes entre las partes, permitiendo por esta vía la garantía del suministro, y de esta forma, darle un importante impulso para el desarrollo de la cadena de valor.

  6. Técnicas para el Modelado de Procesos de Negocio en Cadenas de Suministro Business Process Modelling Techniques in Supply Chains

    Directory of Open Access Journals (Sweden)

    Raquel Sanchis

    2009-01-01

    Full Text Available El presente artículo muestra una revisión y evaluación de las técnicas y metodologías genéricas de modelado de procesos de negocio más populares y utilizadas en la literatura. El principal objetivo del estudio es la propuesta de una taxonomía de clasificación de las técnicas de modelado aplicadas a las características propias de las cadenas de suministro, para facilitar la selección de las más apropiadas. Para verificar la clasificación, se describe un caso real de estudio en cadenas de suministro, en el que se relaciona una cadena de suministro del sector cerámico y una del sector del mueble. Del estudio realizado, se propone un método que incluye cuatro técnicas para la representación de las diferentes perspectivas de modelado en una cadena de suministro.The article shows a review and evaluation of the most popular and meaningful business process modelling techniques and generic methodologies treated in the literature. The main objective is the proposal of a new taxonomy of classification oriented to supply chains, with the aim of facilitating the selection of the most appropriate ones. To verify the classification, a real study case of supply chains is described. In this study, a supply chain from the ceramic sector is related with one from the furniture sector and a method consisting of four modelling techniques to present the different aspects to be considered for representing the different perspectives of the supply chains is proposed.

  7. Analysis of fenbendazole residues in bovine milk by ELISA.

    Science.gov (United States)

    Brandon, David L; Bates, Anne H; Binder, Ronald G; Montague, William C; Whitehand, Linda C; Barker, Steven A

    2002-10-01

    Fenbendazole residues in bovine milk were analyzed by ELISAs using two monoclonal antibodies. One monoclonal antibody (MAb 587) bound the major benzimidazole anthelmintic drugs, including fenbendazole, oxfendazole, and fenbendazole sulfone. The other (MAb 591) was more specific for fenbendazole, with 13% cross-reactivity with the sulfone and no significant binding to the sulfoxide metabolite. The limit of detection of the ELISA method in the milk matrix was 7 ppb for MAb 587 and 3 ppb for MAb 591. Fenbendazole was administered in feed, drench, and paste form to three groups of dairy cattle. Milk was collected immediately before dosing and then every 12 h for 5 days. The ELISA indicated that residue levels varied widely among individual cows in each group. Fenbendazole levels peaked at approximately 12-24 h and declined rapidly thereafter. Metabolites were detected at much higher levels than the parent compound, peaked at approximately 24-36 h, and declined gradually. Residue levels were undetectable by 72 h. The ELISA data correlated well with the total residues determined by chromatographic analysis, but the use of the two separate ELISAs did not afford an advantage over ELISA with the single, broadly reactive MAb 587. The ELISA method could be used to flag high-residue samples in on-site monitoring of fenbendazole in milk and is a potential tool for studying drug pharmacokinetics.

  8. Propuesta de un procedimiento para el diagnóstico de la cadena de suministro de la Empresa Pesquera de Sancti Spíritus.

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    DamarisTaydi Castillo Jiménez

    2015-12-01

    Full Text Available La presente investigación se desarrolla con el objetivo de proponer un procedimiento para diagnosticar la cadena de suministro. En consecuencia se propone la metodología del Modelo SCOR para dar cumplimiento al objetivo propuesto. El mismo se aplica en la Empresa Pesquera de Sancti Spíritus (PESCASPIR.Los resultados del diagnóstico permitieron identificar aquellas oportunidades de mejora que mayor incidencia tienen sobre el desempeño de la cadena, lográndose además un marco único que une los procesos de negocio, los indicadores de gestión, las mejores prácticas y las tecnológicas, en una estructura unificada para mejorar la comunicación entre los miembros de la cadena de suministros. Para el estudio se utilizaron técnicas de análisis, de recopilación de información, organigramas y otros.

  9. Contexto y Caracterización de la Cadena de Suministro del Limón Persa (Citrus latifolia Tanaka en Veracruz-México

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    Gregorio Fernández-Lambert

    2015-01-01

    Full Text Available Esta investigación se realiza en el Distrito Citrícola de Martínez de la Torre, Ver., con alcance a los municipios de Álamo Temapache, y Cuitláhuac en Veracruz, y reporta el contexto e integración de la cadena de suministro del limón persa. El estudio de sus eslabones permitió identificar las problemáticas de abastecimiento, operación y distribución en dicha cadena. A partir de entrevistas abiertas no estructuradas a especialistas, técnicos, productores y empresarios, así como la consulta de bases de datos y documentos de organismos afines a esta agroindustria, los resultados de la investigación advierten que la fuente de los problemas estructurales de la ineficacia en la sincronización de la cadena de suministro del limón persa, se centra en la baja productividad del huerto, un excesivo intermediarismo entre el huerto y la exportadora, la baja adopción tecnológica o uso de tecnología hechiza en los procesos de empaque del cítrico.

  10. Modelos para la planificación centralizada de la producción y el transporte en la cadena de suministro: una revisión

    Directory of Open Access Journals (Sweden)

    Josefa Mula

    2010-08-01

    Full Text Available Este artículo presenta una revisión bibliográfica sobre los modelos de programación matemática para la planificación centralizada de la producción y el transporte en una cadena de suministro. El propósito de esta revisión es identificar la investigación actual y futura en este campo y proponer un marco de clasificación basado en los elementos siguientes: estructura de la cadena de suministro, nivel de decisión, enfoque de modelado, propósito, información intercambiada y aplicación práctica. El objetivo del artículo es proporcionar un punto de partida para el estudio de los problemas de planificación de la producción y el transporte de la cadena de suministro orientado a los investigadores de gestión de la producción.

  11. Análisis de la capacidad de servicio de mantenimiento de la cadena TRD Caribe Sancti Spíritus usando la simulación.

    Directory of Open Access Journals (Sweden)

    Manuel Hung Varela

    2014-09-01

    Full Text Available La investigación, tiene su origen en la necesidad de conocer, si la unidad de servicio de mantenimiento en la Cadena TRD Caribe Sancti Spíritus, es capaz de satisfacer o no las demandas de la Cadena, ya que se presenta una tendencia al crecimiento en la demanda, así como en las quejas por parte de los clientes que aseveraban largas esperas para ser servidos. Se definió el procedimiento para la simulación por eventos discretos a utilizar (Barceló Bugeda, 1996. Se definieron las fronteras del sistema, se construyó el modelo matemático para el sistema objeto de estudio y se programó en el software especializado Promodel 4.0. Para la simulación del modelo matemático, se recopiló información a través de técnicas como el muestreo aleatorio. El modelo fue verificado y validado (Barceló Bugeda, 1996; Aracil Santonja, 1995. Se simuló el modelo, haciendo uso del método de los lotes. Se procesaron los resultados de la simulación, y se concluyó que la unidad de servicio de mantenimiento no dispone de capacidad suficiente bajo las condiciones actuales para satisfacer las demandas de la cadena TRD Caribe en el territorio de Sancti Spíritus.

  12. UltramicroELISA para la detección de anticuerpos IgM anti M. leprae UltramicroELISA assay for the detection of human IgM antibodies to M. leprae

    OpenAIRE

    José Laferte; Elba G. Abreu; René Robaina; Vicente Verez

    1991-01-01

    La disponibilidad del sistema Ultramicroanalítico (SUMA) y de un antígeno especie-específico del M. leprae obtenido mediante síntesis química, permitió la normalización y validación de un ultramicroELISA para la detección de anticuerpos IgM específicos a esta micobacteria. El análisis de 433 sueros de banco de sangre y 265 sueros usados para validar el método y clasificados en un grupo control de donantes de banco de sangre (100), un grupo de pacientes tuberculosos (50), un grupo de enfermos ...

  13. Evaluation of an ELISA for the diagnosis of brucellosis.

    Science.gov (United States)

    Mathai, E; Singhal, A; Verghese, S; D'Lima, D; Mathai, D; Ganesh, A; Thomas, K; Moses, P

    1996-06-01

    Brucela serology using ELISA and standard agglutination test was performed on 23 patients with prolonged fever where the test was requested, on 26 randomly chosen patients with prolonged fever where it was not requested and on 17 controls. ELISA was positive in 39.1, 26.9 and 0 per cent respectively in these groups. Brucellosis may often be unsuspected because of its varied clinical manifestations and may be a more important cause of fever than previously considered. Our data reaffirm that ELISA is superior to the standard agglutination test for the diagnosis of brucellosis.

  14. Análisis de la cadena de valor del queso manchego en Cuenca, España Analisys of the manchego cheese value chain in Cuenca, Spain

    Directory of Open Access Journals (Sweden)

    Brenda Inoscencia Trejo Téllez

    2011-08-01

    Full Text Available El concepto de cadena de valor, se ha desarrollado como respuesta a la necesidad para que las empresas rurales, cumplan con las exigencias de la demanda de productos especializados de alta calidad. En este trabajo se realiza un análisis de la cadena de valor del queso manchego en Cuenca, España; identificando a los agentes y las relaciones entre ellos; en donde se tiene una importante producción de queso manchego con denominación de origen. La investigación se llevó a cabo durante 2008 en la Provincia de Cuenca, España. La metodología que se utiliza incluye las siguientes fases: 1 identificación del sector; 2 identificación de agentes; 3 análisis de la cadena de valor; y 4 análisis de agentes. Los resultados obtenidos presentan que los principales factores que están influyendo en el desarrollo de la cadena de valor son: integración horizontal y vertical, confianza entre agentes, acceso y uso de financiamiento, cumplimiento de normas gubernamentales, cumplimiento de normas de la denominación de origen. Por lo anterior, se concluye que dicha cadena está funcionando correctamente y pueden tomarse elementos de éxito para adecuarse a otros territorios.The value chain concept has been developed as a response to the need for rural enterprises to comply the demand requirements of high quality specialty products. This paper provides an analysis of the value chain of Manchego cheese in Cuenca, Spain; identifying actors and relations between them, where there is a significant production of Manchego cheese with designation of origin. The research was conducted during 2008 in the Province of Cuenca, Spain. Methodology used includes the following phases: 1 sector identification; 2 agents identification; 3 analysis of value chain; and 4 analysis of agents. The results obtained show that the main factors that are influencing the development of the value chain are: horizontal and vertical integration, trust between agents, access to and use of

  15. FUNCIONALIDADES DE SISTEMAS DE PLANIFICACIÓN DE RECURSOS EMPRESARIALES PARA CADENAS DE SUMINISTRO / FUNCTIONALITIES OF ENTERPRISE RESOURCE PLANNING SYSTEMS FOR SUPPLY CHAIN INTEGRATION

    Directory of Open Access Journals (Sweden)

    Dania Pérez-Armayor

    2013-05-01

    Full Text Available En la actualidad los sistemas de planificación de recursos empresariales, además de facilitar las relaciones y la eficiencia intra-empresarial, incluyen elementos para viabilizar la colaboración entre empresas, como respuesta a la necesidad de las organizaciones de agruparse en cadenas de suministro. En consecuencia, el Ministerio de la Informática y las Comunicaciones, junto a otras organizaciones cubanas, están proyectando el desarrollo de un sistema de planificación de recursos empresariales desarrollado en el país (Sistema Cedrux, incluyendo funcionalidades que soporten la integración de cadenas de suministro. Este trabajo se centra en el análisis de funcionalidades que debe poseer el Sistema Cedrux para facilitar una gestión de la cadena de suministro exitosa. Para complementar este análisis fueron realizadas búsquedas bibliográficas y entrevistas a personal participante en el desarrollo de Cedrux. Como resultado se propone la configuración de los módulos acorde al rol de la empresa en la cadena, incluyendo módulos de gestión de cadenas de suministroAbstractNowadays, enterprise resource planning systems (ERP systems, in addition to help improving the relations and the intra-enterprise efficiency, include elements that facilitate inter-enterprise cooperation in response to the need for organizations to come together in supply chains. As a consequence, the Ministry of Informatics and Communications, along with other Cuban organizations, are planning the development of an ERP system developed in Cuba (named Cedrux system, which includes functionalities that support the integration of supply chains. This paper aims to analyze the functionalities that Cedrux should have in order to support a successful supply chain management. Bibliographic searches and interviews to Cedrux developers were conducted, in order to complement this analysis. As a result is suggested the inclusion of modules for supply chain management and the

  16. MODELO MATEMÁTICO PARA LA PLANIFICACIÓN DE LA PRODUCCIÓN EN LA CADENA DE SUMINISTRO

    Directory of Open Access Journals (Sweden)

    Caridad González Sánchez

    2004-07-01

    Full Text Available

    La planificación de la producción dentro de la cadena de suministro es una actividad de especial importancia en estos momentos, debido a que permite la optimización de las operaciones de producción y distribución, y satisfacer la mayoría de los pedidos de los clientes en el tiempo requerido y a un costo más bajo. La aplicación de las técnicas matemáticas en este proceso garantiza una toma de decisiones más rápida y eficiente. En el presente trabajo se propone un modelo matemático multiobjetivo que permite satisfacer las demandas de los clientes determinando la cantidad de recursos necesarios para llevar a cabo la producción lo que redundará en la disminución de los niveles de inventarios.

  17. Cadenas internacionales de valor: alternativa para la integración profunda de América Latina con India

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    Soraya Caro Vargas

    2012-11-01

    Full Text Available Si América Latina desea evitar con las economías emergentes del Asia la repetición del ciclo de dependencia económica –caracterizado por la exportación de materias primas y la importación de manufacturas– necesita identificar y promover alianzas de capital para la construcción de cadenas internacionales de acumulación apalancadas en las inversiones indias y chinas, a fin de producir valor agregado en los dos continentes. Para ello precisa que la Intelligentsia supere los paradigmas impuestos por las cargas ideológicas anteriores a los años noventa que aún prevalecen en muchos analistas asiáticos respecto a Latinoamérica, así como también los que en nuestro medio justifican la política comercial de las grandes naciones de oriente dentro de una suerte de idolatría por las nuevas potencias emergentes.

  18. Cadenas agroalimentarias orgánicas en el sur de chile: tensiones que condicionan su puesta en valor

    Directory of Open Access Journals (Sweden)

    Sandra Ríos Núñez

    2016-01-01

    Full Text Available El modelo industrial de producción de alimentos ha incentivado la emergencia de cadenas agrarias alternativas. Estas suponen modelos de producción fuertemente vinculados al territorio y tienen la finalidad de transitar desde la producción industrial a la captura de valor por medio de sistemas de producción que garanticen la seguridad y soberanía alimentaria. Esta investigación analiza el funcionamiento de la agricultura orgánica en Chile, en tanto producción agraria no convencional, con la finalidad de perfilar su desarrollo como una estrategia de captura de valor y producción de alimentos saludables especialmente para pequeños pro- ductores agrarios. Se busca visibilizar modelos de producción que reconectan el flujo de producción-circulación-consumo a través de un mecanismo de gobernanza que posibilite la captura de valor en el territorio.

  19. Ultrasensitive ELISA using enzyme-loaded nanospherical brushes as labels.

    Science.gov (United States)

    Qu, Zhenyuan; Xu, Hong; Xu, Ping; Chen, Kaimin; Mu, Rong; Fu, Jianping; Gu, Hongchen

    2014-10-01

    Improving the detection sensitivity of enzyme-linked immunosorbent assay (ELISA) is of utmost importance for meeting the demand of early disease diagnosis. Herein we report an ultrasensitive ELISA system using horseradish peroxidase (HRP)-loaded nanospherical poly(acrylic acid) brushes (SPAABs) as labels. HRP was covalently immobilized in SPAABs with high capacity and activity via an efficient "chemical conjugation after electrostatic entrapment" (CCEE) process, thus endowing SPAABs with high amplification capability as labels. The periphery of SPAAB-HRP was further utilized to bind a layer of antibody with high density for efficient capture of analytes owing to the three-dimensional architecture of SPAABs. Using human chorionic gonadotrophin (hCG) as a model analyte, the SPAAB-amplified system drastically boosted the detection limit of ELISA to 0.012 mIU mL(-1), a 267-fold improvement as compared to conventional ELISA systems.

  20. The difference of ELISA and LABScreen in detecting HLA antibodies

    Institute of Scientific and Technical Information of China (English)

    吴萍萍

    2013-01-01

    Objective To compare the difference of ELISA and LABScreen in detecting HLA antibodies and evaluate their effects on allograft rejection.Methods Consecutive patients undergoing kidney transplantion from November,2008 to December,2009 in the First Affiliated Hospital

  1. ELISA reader does not interfere by mobile phone radiofrequency radiation

    OpenAIRE

    Mortazavi, Seyyed Mohammad Javad; Baradaran-Ghahfarokhi, Hamid Reza; Abdi, Mohammad Reza; Baradaran-Ghahfarokhi, Milad; Mostafavi, Nayyer Sadat; Mahmoudi, Golshan; Berenjkoub, Nafiseh; Akmali, Zahra; Hossein-Beigi, Fahimeh; Arsang, Vajiheh

    2016-01-01

    Background: The increasing number of mobile phones can physically cause electromagnetic interference (EMI) in medical environments; can also cause errors in immunoassays in laboratories. The ELISA readers are widely used as a useful diagnostic tool for Enzymun colorimetric assay in medicine. The aim of this study was to investigate whether the ELISA reader could be interfered by the exposure to the 900 MHz cell phones in the laboratory. Materials and Methods: Human serum samples were collecte...

  2. An ELISA test for the detection of antibodies to Legionella pneumophila.

    OpenAIRE

    Wreghitt, T. G.; Nagington, J.; Gray, J

    1982-01-01

    An enzyme-linked immunosorbent assay (ELISA) test has been developed to detect antibodies to Legionella pneumophila serogroup 1. There is good correlation between indirect fluorescent antibody (IFA) and ELISA titres but ELISA is more sensitive.

  3. Serodiagnosis of Oesophagostomum Columbianum infection in Goats using indirect ELISA

    Directory of Open Access Journals (Sweden)

    Fayaz Ahmad

    Full Text Available To determine the seroprevalence of Oesophagostomum columbianum antibodies in goats in Kashmnir, a study was conducted in Srinagar and Ganderbal Districts, located in central parts of Kashmir valley. ELISA was standardized and evaluated to detect goat Oesophagostomosis in experimental and clinical cases using somatic whole adult antigen of Oesophagostomum columbianum. Plate ELISA was standardized using 5μg/well antigen concentration with 1:100 and 1:1000 of sera and conjugate dilution. Indirect plate ELISA was able to demonstrate the antibody titer at different weeks post infection in experimental goats. A comparison of plate ELISA on suspected field sera and faecal sample examination by floatation method revealed that 68 (34.34% samples were found to be positive using ELISA and twelve by faecal examination. The highest prevalence was found in lower age groups in both the seasons, with the increase in age, the infection level decreased. The prevalence was higher in wet season as compared to dry season. The results were stastically significant (P < 0.05. No sex predisposition was seen. Sensitivity and specificity of ELISA was 80.0%, and 21.42%, respectively. This test is therefore quite sensitive for clinical cases; an early diagnosis, however lacks specificity of faecal examination were 10.81% and 90.0%, respectively. [Vet. World 2011; 4(11.000: 503-506

  4. Adsorción de boro mediante perlas de alginato

    OpenAIRE

    Seira Ibáñez, Juana

    2008-01-01

    En este proyecto se propone la técnica de la adsorción mediante la utilización de polímeros naturales para eliminar el boro de residuos industriales, puesto que estos residuos presentan una gran problemática medioambiental. El polímero elegido para realizar la adsorción en este estudio es el alginato. Para poder trabajar en estado sólido se transforma el alginato de sodio, que es soluble en agua, en gel mediante la fabricación de las perlas de alginato de calcio. (Se utiliza...

  5. Desarrollo comunicación Alfa Arduino mediante Scada

    OpenAIRE

    Trallero Calvo, Jorge

    2015-01-01

    El siguiente proyecto pretende crear un sistema IoT (Internet of Things) girando en torno a Arduino, demostrando de este modo la facilidad de creación de proyectos y fiabilidad que nos aporta Arduino. Mediante una comunicación TCP/IP se procede a crear un sistema el cual es capaz de registrar valores a una cierta distancia mediante protocolos de procesos y comunicación (Telemedida) y de este modo monitorizar la temperatura en un punto determinado, sin estar limitado por la t...

  6. Development of an ELISA for the Detection of Azaspiracids.

    Science.gov (United States)

    Samdal, Ingunn A; Løvberg, Kjersti E; Briggs, Lyn R; Kilcoyne, Jane; Xu, Jianyan; Forsyth, Craig J; Miles, Christopher O

    2015-09-01

    Azaspiracids (AZAs) are a group of biotoxins that cause food poisoning in humans. These toxins are produced by small marine dinoflagellates such as Azadinium spinosum and accumulate in shellfish. Ovine polyclonal antibodies were produced and used to develop an ELISA for quantitating AZAs in shellfish, algal cells, and culture supernatants. Immunizing antigens were prepared from synthetic fragments of the constant region of AZAs, while plate coating antigen was prepared from AZA-1. The ELISA provides a sensitive and rapid analytical method for screening large numbers of samples. It has a working range of 0.45-8.6 ng/mL and a limit of quantitation for total AZAs in whole shellfish at 57 μg/kg, well below the maximum permitted level set by the European Commission. The ELISA has good cross-reactivity to AZA-1-10, -33, and -34 and 37-epi-AZA-1. Naturally contaminated Irish mussels gave similar results whether they were cooked or uncooked, indicating that the ELISA also detects 22-carboxy-AZA metabolites (e.g., AZA-17 and AZA-19). ELISA results showed excellent correlation with LC-MS/MS analysis, both for mussel extract spiked with AZA-1 and for naturally contaminated Irish mussels. The assay is therefore well suited to screening for AZAs in shellfish samples intended for human consumption, as well as for studies on AZA metabolism.

  7. Development of an ELISA for the Detection of Azaspiracids.

    Science.gov (United States)

    Samdal, Ingunn A; Løvberg, Kjersti E; Briggs, Lyn R; Kilcoyne, Jane; Xu, Jianyan; Forsyth, Craig J; Miles, Christopher O

    2015-09-01

    Azaspiracids (AZAs) are a group of biotoxins that cause food poisoning in humans. These toxins are produced by small marine dinoflagellates such as Azadinium spinosum and accumulate in shellfish. Ovine polyclonal antibodies were produced and used to develop an ELISA for quantitating AZAs in shellfish, algal cells, and culture supernatants. Immunizing antigens were prepared from synthetic fragments of the constant region of AZAs, while plate coating antigen was prepared from AZA-1. The ELISA provides a sensitive and rapid analytical method for screening large numbers of samples. It has a working range of 0.45-8.6 ng/mL and a limit of quantitation for total AZAs in whole shellfish at 57 μg/kg, well below the maximum permitted level set by the European Commission. The ELISA has good cross-reactivity to AZA-1-10, -33, and -34 and 37-epi-AZA-1. Naturally contaminated Irish mussels gave similar results whether they were cooked or uncooked, indicating that the ELISA also detects 22-carboxy-AZA metabolites (e.g., AZA-17 and AZA-19). ELISA results showed excellent correlation with LC-MS/MS analysis, both for mussel extract spiked with AZA-1 and for naturally contaminated Irish mussels. The assay is therefore well suited to screening for AZAs in shellfish samples intended for human consumption, as well as for studies on AZA metabolism. PMID:26245830

  8. Modificación de la transferencia de nutrientes en la cadena nutritiva microalga-rotífero y microalga-Artemia mediante cambios en la formulación de los nutrientes en el cultivo microalgal

    OpenAIRE

    Ronsón Paulín, José Ángel

    2011-01-01

    Las densidades celulares de los cultivos discontinuo y semicontinuo en Phaeodactylum tricurnutum e Isochrysis galbana var. T-iso, utilizando las diferentes concentraciones de Ca2+, Mg2+, Se2-, Si4+, Fe3+, Zn2+, Cu2+, y S2- produjo diferencias altamente significativas así mismo, en la composición bioquímica y en el conjunto de los ácidos grasos ω-3 y con énfasis en los ácidos grasos eicosapentaenoico (EPA) y docosahexaenoico (DHA) respectivamente. Estos resultados sugirieron que determinadas c...

  9. El fenómeno del Palo de Hockey de las cadenas de suministro en empresas manufactureras: un análisis mediante dinámica de sistemas y experimentos de laboratorio

    OpenAIRE

    Mora Luna, Luz Adriana

    2013-01-01

    Resumen: Cuando es difícil alcanzar la utilidad mensual esperada, los minoristas suelen establecer un sistema de oferta de descuentos para incrementar la demanda del mercado, generándose una acumulación de pedidos al final del reporte mensual de ventas. Esta situación se conoce como el fenómeno del Palo de Hockey. A pesar que la generación de este fenómeno se ha descrito en la literatura desde hace varios años, hay poca investigación que estudie las posibles causas que generan su comportamien...

  10. Individualismo y desconfianza en el trabajo asociativo de cadenas productivas de agroexportacion en el Valle del Mantaro, Junín, Perú

    Directory of Open Access Journals (Sweden)

    William Rodríguez Giráldez

    2014-12-01

    Full Text Available Objetivos: Determinar el individualismo y desconfianza en el trabajo asociativo de cadenas productivas de agroexportación en el Valle del Mantaro. Métodos: Investigación descriptiva, no experimental, de alcance transeccional correlacional. Fueron encuestados 383 productores agrícolas de las provincias que comprenden el Valle del Mantaro: Huancayo, Concepción, Jauja y Chupaca. Se utilizaron los estadísticos descriptivos, distribución de frecuencias, tablas de contingencia, y chi cuadrado para prueba de hipótesis. Resultados: En la dimensión individualismo: 72,23 % de productores manifestaron una actitud positiva hacia cadenas productivas; 87,2 % estimaron importante participar en reuniones; 88,5 % consideraron importante la capacitación en asociatividad y 86,2 % gustan trabajar conjuntamente con vecinos. Se deduce que el 83,53 % de ellos no tienen el rasgo de individualismo; por tanto, solo el 16,47 % tienen este rasgo y no influye negativamente en el trabajo asociativo en el Valle del Mantaro. En la dimensión desconfianza: 52 % de productores agrícolas manifestaron buena comunicación en la cadena productiva; 40 % aportaron entre 50 % y 100 % de costos de producción; 56 % podrían aportar entre 50 % y 100 % de costos de producción; 60 % señalaron que confían en empresas articuladoras y 60 % confían en colegas productores. Se deduce que, 53,6 % de estos productores no poseen el rasgo de desconfianza; mientras que 46,4 % si los tienen. Conclusiones: Solo 16,47 % de productores agrícolas tienen rasgos de individualismo y no influye negativamente en el trabajo asociativo; 46,4 % de estos productores tienen el rasgo de desconfianza y este valor sí influye negativamente en el trabajo asociativo en el valle.

  11. Cellulose acetate as solid phase in ELISA for plague

    Directory of Open Access Journals (Sweden)

    Barbosa AD

    2000-01-01

    Full Text Available Antigen from Yersinia pestis was adsorbed on cellulose acetate discs (0.5 cm of diameter which were obtained from dialysis membrane by using a paper punch. ELISA for human plague diagnosis was carried out employing this matrix and was capable to detect amount of 1.3 µg of antigen, 3,200 times diluted positive serum using human anti-IgG conjugate diluted 1:4,000. No relevant antigen lixiviation from the cellulose acetate was observed even after washing the discs 15 times. The discs were impregnated by the coloured products from the ELISA development allowing its use in dot-ELISA. Furthermore, cellulose acetate showed a better performance than the conventional PVC plates.

  12. Competitividad del sistema agroalimentario de la cebolla de bulbo con enfoque de cadena productiva en el distrito de riego del alto chicamocha (boyacá)

    OpenAIRE

    Melo, Ligia Ines; Melo, María Mercedes; Rodríguez, Luis Felipe

    2010-01-01

    El análisis de la competitividad plantea una pregunta lógica sobre los determinantes que la originan, percibida ésta como un objeto móvil y, por tanto, que debe ser comprendida en términos de su dinámica. Por su importancia, el presente artículo se orienta al análisis de la competitividad del sistema agroalimentario de la cebolla de bulbo en el Distrito de riego del Alto Chicamocha (Boyacá), bajo el enfoque de cadena productiva, haciendo énfasis en los eslabones de producción y comercializaci...

  13. Análisis de la cadena productiva de la ganadería bovina de doble propósito en la costa oaxaqueña.

    OpenAIRE

    Flores Verduzco, Samuel

    2011-01-01

    Una de las principales cadenas productivas rurales del país lo constituye la ganadería bovina productora de carne, la cual no satisface la demanda nacional del producto, debido a que se necesita importar cantidades significativas (hasta 40% del consumo para años específicos). Por otra parte, el país exporta alrededor de 1.5 millones de becerros en pie y novillonas al año. El objetivo del trabajo fue realizar un diagnóstico de la actividad ganadera productora de carne de bovino en la Costa de ...

  14. Modelización de los indicadores de gestión en la cadena de suministro. una visión sistémica

    OpenAIRE

    MARTÍN DARIO ARANGO SERNA; GIOVANNY PEREZ ORTEGA; MIGUEL DAVID ROJAS LOPEZ

    2008-01-01

    El modelo que describe este artículo, pretende medir, analizar, evaluar y plantear un mejoramiento de los procesos administrativos, identificando las diferentes variables que intervienen en la gestión de la cadena de suministro centrándose en el subsistema de producción. Este trabajo hace parte de una investigación empírica que se viene desarrollando en el sector textil. El modelo muestra de forma empírica las relaciones que existen entre, las funciones internas de la empresa en el área de la...

  15. El papel de las TIC en el rendimiento de las cadenas de suministro: el caso de las grandes empresas de España y Portugal

    OpenAIRE

    Nicolás Gonzálvez- Gallego; Pedro Soto-Acosta; António Trigo; Francisco José Molina- Castillo; João Varajão

    2010-01-01

    Este artículo analiza las diferencias existentes entre las grandes empresas de España y Portugal en lo referido tanto al uso de las Tecnologías de la Información y la Comunicación (TIC) como a la integración electrónica con los agentes de la cadena de suministro. Estas diferencias se establecen en términos de la capacidad para generar resultados empresariales superiores. Los resultados muestran diferencias entre ambos grupos de empresas y confirman que las empresas españolas deberían persegui...

  16. Desarrollo de un juego de simulación sobre gestión de riesgos en la cadena de suministro

    OpenAIRE

    García Colorado, Jaime

    2012-01-01

    El objetivo del proyecto es poner en práctica el diseño conceptual de un juego didáctico de simulación sobre gestión de inventarios de una cadena de suministro para hacer frente a sucesos de baja probabilidad de ocurrencia y gran impacto. Para la puesta en funcionamiento, se parte de unos requisitos de partida: - Debe proporcionarse un soporte informático sencillo que permita realizar el juego en una clase con alumnos distribuidos en equipos que compiten de dos en dos. - Los requisitos de fun...

  17. An ELISA for the quantitation of von Willebrand Factor

    DEFF Research Database (Denmark)

    Vinholt, Pernille Just; Overgaard, Martin; Diederichsen, Axel Cosmus Pyndt;

    2013-01-01

    with and without documented coronary calcification (total n=118). RESULTS AND CONCLUSIONS: The assay detected VWF:OPG complexes in human plasma, while no significant signal was observed when testing solutions containing VWF or recombinant OPG alone. Importantly, the ELISA assay was able to detect in vitro formed...... for measurement of von Willebrand factor-osteoprotegerin complex (VWF:OPG) in human plasma. Furthermore, the significance of VWF:OPG complex as a marker of cardiovascular disease (CVD) was evaluated. PATIENTS/METHODS: A sandwich ELISA for quantification of VWF:OPG was developed using a polyclonal rabbit anti...

  18. [The systematization of the enzyme-linked immunosorbent assay (=ELISA)].

    Science.gov (United States)

    Gränzer, W

    1989-06-01

    Using the example of the detectability of immunological methods the study shows the considerable influence exerted by this detectability on the quantitative and qualitative parameters and the necessity of a systematization of the methods. This systematization already starts with the product labeling: A sufficient description of antibodies has to contain five qualities. The profitability of the experimental research is improved by optimized product and method systematization. ELISA is presented in a systematized form as a modular system optimizing the comparability of these methods. In order to detect the gain of information transparency obtained by systematization, an ELISA, described in the literature, is presented in the original and in the systematized form.

  19. Comparative evaluation of competitive ELISA test in Colombian cattle

    International Nuclear Information System (INIS)

    In order to contribute to the definition of the best ELISA test for screening and differential diagnosis of Brucella abortus to be applied for control programmes, a total of 2971 sera from Colombian cattle were tested for brucellosis. Conventional agglutination tests, Buffered Plate antigen test (BPAT) and Rose Bengal (RB) as well as Complement Fixation test (CFT) (Alton, et al. 1988) were used comparatively. Radial immunodiffusion test (RID) was also performed to all sera. The sera were also tested using four different ELISAs: indirect ELISA from FAO/IAEA and the indirect ELISA modified by Nielsen, et al. 1992 as well as two competitive ELISAs: one competitive ELISA used B. abortus O-polysaccharide antigen and an enzyme conjugated monoclonal to the O-polysaccharide for competition and detection. The second competitive ELISA used lipopolysaccharide (sLPS) antigen, a different monoclonal antibody for competition but also specific for the O-polysaccharide and a commercially available goat anti-mouse IgG enzyme conjugate for detection. The sera were analyzed based on its population status, 987 positive obtained from Brucella abortus infected herds based on clinical and/or bacteriological evidence and a high prevalence of brucellosis, CFT percentage of positive animals in the herd was greater than 5%. Eight hundred sixty six (866) negative sera from non-vaccinated cattle from a brucellosis free area and 1118 negative sera obtained from reglamentary vaccinated areas under a free herd program. Initial cut-off values were derived using negative serum samples. The diagnostic sensitivity and specificity was defined from frequency histograms based on this cut-off values and using 2x2 tables, corresponding confidence limits (95%) were calculated. The data were also analysed using signal detection analysis (ROC). Kappa statistics was determined for all tests and populations, accuracy was used as index of comparison to evaluate different assays. The data support the initial

  20. La cadena de suministro del sector metalúrgico: un análisis de sus principales eslabones (The Supply Chain in the metallurgical sector: an analysis of the main links

    Directory of Open Access Journals (Sweden)

    Patricia Carbajo-de-Lera

    2011-12-01

    Full Text Available Los directivos actuales conciben que la competencia ya no es empresa frente a empresa, sino que desborda este plano generándose una competencia cadena de suministro frente a cadena de suministro. Por ello, la gestión de la cadena de suministro se convierte en un elemento clave para la supervivencia empresarial y la obtención de ventajas competitivas. El diseño de la cadena de suministro viene definido, en buena parte, por las características del sector al que pertenece. En consecuencia, es de interés realizar análisis sectoriales. El presente trabajo tiene como objetivo analizar la cadena de suministro en un sector de gran importancia en la economía española, como es el metalúrgico. En concreto, buscamos determinar su cadena de suministro, definiendo los principales eslabones que la configuran y las relaciones entre ellos.Abstract: In the current business context, competition is no longer firm against firm, but has moved on to a higher plane, with the generation of supply chain against supply chain competition.  Therefore, Supply Chain Management (SCM has strategic relevance as a source of competitive advantage. The design of the supply chain is defined largely by the characteristics of the sector. Consequently, sectorial analysis is required. The aim of this paper is to analyze the supply chain of the metallurgical sector. Specifically, it will be focused on determining its supply chain and defining its main links and relationships.

  1. Consecuencias del efecto Bullwhip según distintas estrategias de gestión de la cadena de suministro: modelado y simulación = Bullwhip Effect Consequences according to Different Supply Chain Management Strategies: Modelling and Simulation

    Directory of Open Access Journals (Sweden)

    Campuzano Bolarín, Francisco

    2008-01-01

    Full Text Available Consecuencias del efecto Bullwhip según distintas estrategias de gestión de la cadena de suministro: modelado y simulación = Bullwhip Effect Consequences according to Different Supply Chain Management Strategies: Modelling and SimulationAbstract: El efecto Bullwhip es uno de los principales causantes de las inestabilidades en el proceso de gestión de demanda que se producen a lo largo de la cadena de suministro. El presente artículo expone un modelo capaz de recrear diferentes escenarios para la gestión de demanda en una cadena de suministro determinada, con independencia del número de niveles definidos en la cadena de suministro considerada. El modelo, realizado utilizando la metodología de la dinámica de sistemas, incorpora las variables necesarias para simular dicho proceso de gestión de demanda, como por ejemplo: niveles de inventario, órdenes de reabastecimiento, fabricación, previsiones u otras. Se muestra la utilidad del modelo propuesto, comparando los resultados que ofrecen dos escenarios diferentes, como son los representados por una cadena tradicional y el de una cadena reducida. = The Bullwhip effect is one of the main causes of instability in the management demand process along the Supply Chain. We introduce a model which is able to reproduce different Supply Chain Management Scenarios within a determinate Supply Chain with whichever the levels of this one. The model has been built using Systems Dynamics Methodology and incorporates the main variables which are required for simulating the Management Demand Process (Inventory levels, Replenishment orders, manufacturing process, forecasting etc.. This paper demonstrates the utility of the proposed model comparing the results offered by two different scenarios namely Traditional supply chain and Reduced supply chain.

  2. Study of Immunoassay Methods for Recombinant Human Erythropoietin(rhEPO)Using Competitive ELISA

    Institute of Scientific and Technical Information of China (English)

    Jin YAN; Jie Bo MI; Wen Bao CHANG

    2004-01-01

    Two different immunoassay methods, competitive indirect enzyme-linked immuno- sorbent assay (CI-ELISA) and amplificative competitive indirect ELISA (ACI-ELISA) using biotin-avidin complex system were studied to detect rhEPO.The linear ranges were 50-20000 ng/mL and 10-50000 ng/mL for CI-ELISA and ACI-ELISA, respectively.The low detection limits of CI-ELISA and ACI-ELISA were 62.8 ng/mL and 8.5 ng/mL, respectively.

  3. Consecuencias del efecto Bullwhip según distintas estrategias de gestión de la cadena de suministro: modelado y simulación = Bullwhip Effect Consequences according to Different Supply Chain Management Strategies: Modelling and Simulation

    OpenAIRE

    CAMPUZANO BOLARÍN, FRANCISCO; Lario Esteban, Francisco Cruz; Ros McDonnell, Lorenzo

    2008-01-01

    Consecuencias del efecto Bullwhip según distintas estrategias de gestión de la cadena de suministro: modelado y simulación = Bullwhip Effect Consequences according to Different Supply Chain Management Strategies: Modelling and SimulationAbstract: El efecto Bullwhip es uno de los principales causantes de las inestabilidades en el proceso de gestión de demanda que se producen a lo largo de la cadena de suministro. El presente artículo expone un modelo capaz de recrear diferentes escenarios para...

  4. Impacto del uso de las tecnologías de la información y la comunicación que apoyan la cadena de suministro (TIC-CS) sobre el desempeño organizacional

    OpenAIRE

    Víctor Andrés Peñates Montes

    2015-01-01

    Este trabajo, por medio de una revisión de la literatura relevante, pretende determinar y analizar la naturaleza y la forma que tienen los impactos sobre el desempeño organizacional generados por el uso de las tecnologías de la información y la comunicación que apoyan la cadena de suministro (TIC-CS). Primero se realiza un breve esbozo de los conceptos de desempeño y de cadena de suministro, luego se revisan y discuten los resultados de veintiséis investigaciones empíricas relacionadas con di...

  5. ELISA reader does not interfere by mobile phone radiofrequency radiation

    Directory of Open Access Journals (Sweden)

    Seyyed Mohammad Javad Mortazavi

    2016-01-01

    Conclusion: This study showed that ELISA reader does not interfere by mobile phone RF radiation at a closed contact (less than 5 cm distance. However, we recommend that medical institutions discuss these issues in the context of their specific use of technologies and frame a policy that is clear and straightforward to guide staff, patients, and visitors.

  6. Rapid screen for truncating ATM mutations by PTT-ELISA

    Energy Technology Data Exchange (ETDEWEB)

    Du Liutao; Lai, C.-H. [Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, CA 90095 (United States); Concannon, Patrick [Department of Biochemistry and Molecular Genetics, University of Virginia, VA 22908 (United States); Gatti, Richard A. [Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, CA 90095 (United States); Department of Human Genetics, David Geffen School of Medicine at UCLA, CA 90095 (United States)], E-mail: rgatti@mednet.ucla.edu

    2008-04-02

    Mutations in the ataxia-telangiectasia mutated (ATM) gene are responsible for the autosomal recessive genetic disorder, ataxia-telangiectasia (A-T). Approximately 80% of ATM mutations found in A-T patients results in truncations, which can be detected by Protein Truncation Test (PTT). Conventional PTT uses SDS-PAGE electrophoresis to detect mobility of radiolabeled truncated protein fragments. In this study, we developed a non-radioactive Protein Truncation Test which utilizes an enzyme-linked immunosorbent assay (PTT-ELISA) to detect ATM mutations in eight overlapping fragments. N- and C-terminal epitopes (c-myc and V5, respectively) were introduced into transcription/translation products, which could then be detected by Sandwich ELISA. Using this assay, we screened 9 newly diagnosed A-T patients consecutively. Of the 18 expected mutations, 14 truncating mutations were independently identified by cDNA direct sequencing and/or DNA dHPLC analysis. PTT-ELISA detected all of these 14. Four mutations were novel. The PTT-ELISA provides a rapid method for detecting truncating mutations in large genes and should be considered prior to using more laborious or costly methods, such as direct sequencing.

  7. Consolidación de carga como mecanismo de coordinación en cadenas de suministro de perecederos: Estudio de simulación

    Directory of Open Access Journals (Sweden)

    Juan Pablo Castrellón-Torres

    2015-01-01

    Full Text Available Una de las necesidades más apremiantes del sector primario es el desarrollo de métodos, herramie ntas, modelos y técnicas para u na gestión adecuada y eficiente de productos perecederos a lo largo de Cadenas de Suministro (SC, pa rticularmente enfocados en lo s servicios logísticos especializad os (ThirdParty Logistics 3PL como la consolid ación de carga. Esta estrategia de coordinación busca la alineación de los agentes y procesos para la eficiente manipulación de carga, minimi zando costos y permitiendo el uso adecuado de capacidades de medios de transporte. La cont ribución de este artículo se centra en el modelado y análisis de beneficios de las estrategias de consolidación de carga en el contexto de Cadenas de Suministro Agrícolas (ASC a través de la simulación. Los resultados de la simulación de eventos discretos, evaluando un producto commodity en tres diferentes escen arios, prueba los beneficios de esta e strategia de coordinación al reducir el nú mero anual de envíos de 1732 a 725 en un contex to de logística urbana e incrementando los nivel es de utilización de la flota hasta el 71%.

  8. Evaluación de competencias de las regiones para el desarrollo de un clúster útil a la cadena productiva de pasifloras en Colombia

    Directory of Open Access Journals (Sweden)

    Leila N. Ramírez

    2016-01-01

    Full Text Available En Colombia, el Ministerio de Agricultura y Desarrollo Rural (MADR ha definido las cadenas productivas agrícolas con potencial para alcanzar la prosperidad regional y se encuentra impulsando iniciativas, entre las que está la creación de clústeres, con el fin de lograr ese objetivo. Esta investigación busca hacer explícita la metodología utilizada para definir las capacidades competitivas de las diferentes regiones productoras de pasifloras (frutas de carácter exótico que tiene un alto potencial de desarrollo comercial. Se trata de establecer la mejor ubicación dentro de las regiones potenciales para el clúster en esta cadena en Colombia. Se emplea una matriz de evaluación basada en el diamante de competitividad de Porter. Esto permitió establecer que el Huila es la región que alcanza el mayor nivel competitivo para el desarrollo de este tipo de agrupaciones.

  9. UltramicroELISA para la detección de anticuerpos IgM anti M. leprae UltramicroELISA assay for the detection of human IgM antibodies to M. leprae

    Directory of Open Access Journals (Sweden)

    José Laferte

    1991-12-01

    Full Text Available La disponibilidad del sistema Ultramicroanalítico (SUMA y de un antígeno especie-específico del M. leprae obtenido mediante síntesis química, permitió la normalización y validación de un ultramicroELISA para la detección de anticuerpos IgM específicos a esta micobacteria. El análisis de 433 sueros de banco de sangre y 265 sueros usados para validar el método y clasificados en un grupo control de donantes de banco de sangre (100, un grupo de pacientes tuberculosos (50, un grupo de enfermos de lepra (65 y un grupo de contactos de estos enfermos (50, mostró la especificidad del ensayo para evidenciar la infección con el M. leprae. Los resultados obtenidos del estudio adicional de 140 muestras de suero de contactos de enfermos estuvieron estrechamente correlacionados (r = 0,98 con los resultados obtenidos por la técnica de microELISA convencional. La utilización del SUMA no solo permite un notable ahorro de reactivos si no además facilita la lectura, cálculo, validación y almacenamiento automático de los resultados.The availability of an ultramicroanalitic system (SUMA and specie-specific antigen of M. leprae obtained by chemical synthesis, have made possible the standardization and validation of an ultramicroELISA assay for detecting specific human IgM antibodies to this mycobacterium. The specificity of this test to demonstrate the infection with M. leprae was corroborated through a screening of 433 blood bank serum samples and other 265 from diferent groups (100, control group, 50 tuberculosis patients, 65 leprosy patients, 50 from household. The results obtained in the aditional study of 140 household sero showed a high correlation (r = 0.98 with the conventional microELISA method. The use of SUMA allows saving reagents and time since sample handling, plate reading, print out and storing the data are computer assisted.

  10. A sandwich ELISA for the detection of Wnt5a.

    Science.gov (United States)

    Kummitha, China Malakondaiah; Mayle, Kristine M; Christman, Mark A; Deosarkar, Sudhir P; Schwartz, Anthony L; McCall, Kelly D; Kohn, Leonard D; Malgor, Ramiro; Goetz, Douglas J

    2010-01-31

    Wnt5a is a noncanonical member of the Wnt family of signaling molecules that has been linked to various physiological and pathological processes including cell differentiation, cell migration, cell growth, vascular remodeling, cancer and chronic inflammation. To understand the role of Wnt5a in these processes, it is necessary to determine the function and expression level of Wnt5a. In this study we developed a sensitive and specific sandwich enzyme-linked immunosorbent assay (ELISA) for detecting Wnt5a. We found that a rabbit anti-human Wnt5a is a suitable capture antibody for establishing a sandwich ELISA. We used two systems to detect Wnt5a: (1) goat anti-mouse Wnt5a and horseradish peroxidase (HRP) conjugated F(ab')(2) donkey anti-goat IgG as detection and enzyme-linked antibodies respectively, or (2) biotinylated goat anti-mouse Wnt5a and HRP-streptavidin as detection antibody and enzyme-linked avidin respectively. A sandwich ELISA using either of these systems failed to detect recombinant mouse (rm)-Wnt5a diluted in Hank's balanced salt solution supplemented with Ca(2+) and Mg(2+) and 1% bovine serum albumin (HBBS+, 1% BSA). Addition of polyethylene glycol (PEG) to the HBBS+, buffer during the binding stage of rm-Wnt5a, afforded the detection of rm-Wnt5a. The use of PEG during both the binding of rm-Wnt5a and detection antibody stages of the assay yielded the maximum signal for rm-Wnt5a. The relationship between the ELISA signal and concentration of Wnt5a was linear with an R(2) of 0.9934. In summary, we have developed a specific and sensitive sandwich ELISA that detects rm-Wnt5a. PMID:19919840

  11. E.L.I.S.A. en coccidioidomicosis humana E.L.I.S.A. in human coccidioidomycosis

    Directory of Open Access Journals (Sweden)

    Iris Nora Tiraboschi

    1991-08-01

    Full Text Available Se realizó E.L.I.S.A. con exoantígeno de Coccidioides immitis para la detectión de anticuerpos, en 67 sueros humanos diluidos 1/1000, 1/2000, 1/4000 y 1/8000. De los 18 sueros de enfermos de coccidioidomicosis comprobada por examen directo, cultivo y/o histología, 5 fueron negativos, en otros 13 fueron positivos en una o varias diluciones. 3/26 sueros de personas sanas, coccidioidino positivas, fueron positivos en títulos de 1/1000 y el resto no tuvo anticuerpos detectables. No presentaron reacciones positivas ninguno de los sueros controles de personas sanas, pero sí lo hicieron 4/8 pacientes con otras micosis. Se concluye que E.L.I.S.A. es útil para la detección de mínimas cantidades de anticuerpos o en sueros que no pueden ser procesados por fijación de complemento. No es recomendable el uso de la técnica en forma aislada por al presencia de reacciones cruzadas.An E.L.I.S.A. test for antibody detection, with an exo-antigen of Coccidioides immitis was standardized in 67 humans sera diluited in 1/1000, 1/2000, 1/4000 and 1/8000. Eightheen sera from mycologically proved cases of coccidioidomycosis were studied: 5 were negative and 13 were positive in some dilutions. 3/26 sera of healthy persons who presented positive skin tests with coccidioidin were positive and the other 23 sera did not have positive reactions. None of the 15 sera of healthy human exhibited positive E.L.I.S.A. Serum samples of 8 patients suffering other deep mycosis were studied, 4 of them presented cross-reactions in E.L.I.S.A. tests. E.L.I.S.A. test seems to be a useful Serologic technique for antibody detection in anticomplementary serum samples or when a low concentration of antibodies should be detected. As it is very sensitive, cross-reactions with other mycoses are frequent, thus the use other more specific serologic technique together E.L.I.S.A. is recommended.

  12. Validación y aplicación de la prueba ELISA para medir cortisol fecal en jaguar (Panthera onca y puma (Puma concolor durante un programa de enriquecimiento ambiental en el Zoológico Jaime Duque

    Directory of Open Access Journals (Sweden)

    Catalina Rodríguez Álvarez

    2005-12-01

    Full Text Available El enriquecimiento ambiental busca aumentar el bienestar de los animales cautivos mediante la provisión de estímulos que motiven la realización de comportamientos típicos de la especie. A las poblaciones de jaguares (Panthera onca y pumas (Puma concolor presentes en el Zoológico Jaime Duque se les aplicó un programa de enriquecimiento ambiental y con el fin de probar si su bienestar aumentaba, se realizaron mediciones de cortisol en materia fecal mediante la prueba de ELISA, para lo cual hubo necesidad de validar la técnica para cada especie. La prueba utilizada resultó ser válida para ambas especies; sin embargo, los niveles de cortisol se ven influenciados por múltiples variables y no se hizo evidente la reducción de los niveles del mismo con el enriquecimiento ambiental.

  13. A Protein Microarray ELISA for Screening Biological Fluids

    Energy Technology Data Exchange (ETDEWEB)

    Varnum, Susan M.; Woodbury, Ronald L.; Zangar, Richard C.

    2004-02-01

    Protein microarrays permit the simultaneous measurement of many proteins in a small sample volume and therefore provide an attractive approach for the quantitative measurement of proteins in biological fluids, including serum. This chapter describes a microarray ELISA assay. Capture antibodies are immobilized onto a glass surface, the covalently attached antibodies bind a specific antigen from a sample overlaying the array. A second, biotinylated antibody that recognizes the same antigen as the first antibody but at a different epitope is then used for detection. Detection is based upon an enzymatic signal enhancement method known as tyramide signal amplification (TSA). By coupling a microarray-ELISA format with the signal amplification of tyramide deposition, the assay sensitivity is as low as sub-pg/ml.

  14. Efficacy demonstration of tetanus vaccines by double antigen ELISA.

    Science.gov (United States)

    Rosskopf, U; Noeske, K; Werner, E

    2005-09-01

    This paper describes a double antigen ELISA (DAE) for rapid, specific and reliable assessment of the antitetanus immune status of horses and sheep. Compared with the indirect ELISA, the double antigen ELISA has the advantage of species-independent testing of sera. Thanks to its test design, it is more specific since the detected antibodies are forced to bind tetanus toxoid twice. In addition, it is very sensitive to tetanus antibodies, enabling the detection of low antibody titres, in range which is relevant for the assessment of the protective status (tetanus toxin neutralising antibodies). The detection limit of the DAE for tetanus antibodies is in the order of 10(-4) EU/ml. A comparison of in vitro results of individual sera with in vivo titres showed that horse sera with titres of 0.04 and 0.05 EU/ml in the DAE showed titres of > 0.05 IU and 0.034 IU/ml respectively during in vivo testing thus indicating good agreement. For tested sheep sera which were rated > 0.05 IU/ml in vivo, the corresponding titre in the DAE was 0.24 EU/ml. Clear tetanus antitoxin establishment of protective ELISA limits requires further comparative examination of sera with low titres (marketing authorisation procedures of tetanus vaccines ad us. vet. As a consequence, the toxin neutralisation test (still being the standard method of choice for quantifying tetanus toxin neutralising antitoxin titres) could be replaced, since it requires too great a number of animals per test and involves considerable suffering for the animals. The test described here reduces the use of mice and guinea pigs within vaccine efficacy testing. In addition, it involves less exposure of the laboratory personnel to toxin.

  15. A Sandwich ELISA for the Detection of Wnt5a

    OpenAIRE

    Kummitha, China Malakondaiah; Mayle, Kristine M.; Christman, Mark A.; Deosarkar, Sudhir P.; Schwartz, Anthony L; McCall, Kelly D.; Kohn, Leonard D.; Malgor, Ramiro; Goetz, Douglas J.

    2009-01-01

    Wnt5a is a noncanonical member of the Wnt family of signaling molecules that has been linked to various physiological and pathological processes including cell differentiation, cell migration, cell growth, vascular remodeling, cancer and chronic inflammation. To understand the role of Wnt5a in these processes, it is necessary to determine the function and expression level of Wnt5a. In this study we developed a sensitive and specific sandwich enzyme-linked immunosorbent assay (ELISA) for detec...

  16. CONSIDERACIONES SOBRE LA PLANIFICACIÓN DE PRODUCTOS ALIMENTICIOS EN UNA CADENA DE SUMINISTRO COMERCIAL / CONSIDERATIONS ON FOODSTUFF PLANNING IN A COMMERCIAL SUPPLY CHAIN

    Directory of Open Access Journals (Sweden)

    Neyfe Sablón-Cossío

    2013-09-01

    Full Text Available En la actualidad, la necesidad de pasar a la gestión de cadenas de suministro en la economía cubana se refleja a través de diversos síntomas que expresan la falta de coordinación entre las diversas redes de empresas. El presente trabajo muestra la aplicación de este enfoque a la cadena de suministro del puré de tomate, desde la base productiva nacional que abastece la industria, las importaciones de conservas y su abastecimiento a la red de mercados. Se realiza un análisis de la planificación colaborativa como concepto coordinador de planes conjuntos entre los actores claves en esta red. Como problemática principal se detectan: el desconocimiento de la demanda de los clientes, la inadecuada gestión de los productos en mercados locales y la baja integración de la cadena para el logro de alta disponibilidad. Como resultado se propone la planificación colaborativa como base de la filosofía de gestión para la mejora de la satisfacción del cliente.AbstractNowadays, it is necessary a change to the supply chain management in the Cuban economy, which is showed through many symptoms that express the lack of coordination in the enterprise networks. The present work applies this approach to the tomato paste commercial supply chain, from the national production and industry, the imports of this product and its supply to the network of markets. The collaborative planning is analysed as the main concept for the coordination in this supply chain. There were detected problems such as: the lack of knowledge about the demand, the inappropriate product management in local markets and the low integration of the supply chain in order to reach high levels of availability. As a result, it is proposed the collaborative planning as the basic philosophy in order to improve the client satisfaction.

  17. Simulación de Cadenas de Suministro: Nuevas Aplicaciones y Áreas de Desarrollo Supply Chain Simulation: New Applications and Development Areas

    Directory of Open Access Journals (Sweden)

    José L Calderón

    2007-01-01

    Full Text Available El presente trabajo es una revisión de la literatura sobre simulación de cadenas de suministro y resume los conceptos principales sobre simulación y analiza más de 70 artículos publicados entre los años 2000 y 2006. El objetivo es brindar una revisión crítica para quienes empiezan su investigación en esta área o están interesados en la simulación como herramienta para mejorar la cadena de suministro en los niveles estratégico, táctico y operativo. Selecciona 40 artículos en función de sus nuevos enfoques y aplicaciones, y los clasifica según ocho criterios: objetivos, tipo de análisis, etapas de la cadena de suministro abarcadas, procesos simulados, grado de detalle de cada eslabón, simulación del nivel estratégico, tipo de modelo de simulación, y simulación de modelos de evento discreto. El trabajo concluye determinando nueve áreas de desarrollo de los nuevos enfoques y aplicaciones, de las cuales los sistemas multi-agentes y las estrategias de colaboración y coordinación son los más empleados y estudiadasA literature review on supply chain simulation is presented. The paper summarizes the main concepts on simulation and analyzes more than 70 papers published between the years 2000 and 2006. The objective is to offer a state of the art for those who begin research in this area or are interested in the simulation as a tool to improve the supply chain in the strategic, tactical and operative levels. Fourty articles are selected considering the new approaches and applications, and classifies them according to eight criteria: objectives, analysis type, supply chain stages included, simulated processes, detail of each stage, simulation of the strategic level, type of simulation models, and simulation models of discrete events. The work finishes determining nine development areas of the new approaches and applications, of which the multi-agents systems and the strategies of collaboration and coordination are the most used

  18. Cadenas logísticas de exportación de frutas y desarrollo local en el sureste de México

    Directory of Open Access Journals (Sweden)

    Carlos Martner Peyrelongue

    2006-12-01

    Full Text Available La inserción del Sureste mexicano en el proceso de globalización ha sido difícil. La desaparición de actividades económicas, la exclusión y la migración han sido manifestaciones de su deterioro en la última década. No obstante, la región cuenta con algunos nodos o ámbitos locales específicos cuyas actividades y productos tienen potencial para insertarse en las redes de producción-distribución globalizadas. En este sentido, el presente artículo analiza el desarrollo de la cadena de exportación de una fruta tropical (la piña fresca bajo la hipótesis de que el impulso innovador y fortalecimiento de las redes productivas locales en territorios del sureste mexicano está estrechamente vinculado a la formación de cadenas logísticas, así como al control operativo, tecnológico y espacio-temporal que los actores locales alcanzan sobre las mismas. El planteamiento teórico se apoya en el enfoque sistémico de la cadena logística, que prescinde de las divisiones conceptuales tajantes entre producción, distribución y consumo, al poner énfasis en la circulación, es decir, en los flujos, cuya capacidad de movilización es fundamental para articular, integrar y dar viabilidad a los territorios en un contexto de relaciones globalizadasThe insertion of the Mexican southeast region into the globalization process has been a difficult task. The loss of economic activity, exclusion, and migration have been signs of the region's deterioration in the last decade. Nonetheless, this region contains several local"nodes" or spheres whose products and activities have the potential to participate in global production-distribution networks. This paper analyzes the export chain development of a tropical fruit (pineapple under the hypothesis that innovation support and strengthening of local production networks in southeast Mexico are closely linked to the formation of logistic supply chains and technical and operative control undertaken by local

  19. Dot-ELISA for the diagnosis of neurocysticercosis Dot-ELISA no diagnóstico da neurocisticercose

    Directory of Open Access Journals (Sweden)

    Rakhi Biswas

    2004-10-01

    Full Text Available The aim of the present study was to standardize and evaluate dot-Enzyme linked immunosorbent assay (Dot-ELISA, a simple and rapid test for the detection of cysticercus antibodies in the serum for the diagnosis of neurocysticercosis (NCC. The antigen used in the study was a complete homogenate of Cysticercus cellulosae cysts obtained from infected pigs and dotted on to nitrocellulose membrane. Test sera were collected from the patients of NCC, and control sera from patients with other diseases and healthy students and blood donors of the Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER Hospital, Pondicherry, during a study period from 2001 to 2003. Dot-ELISA detected antibodies in 14 of 25 (56% in clinically suspected cases of NCC, 13 of 23 (56.5% in CT/MRI proven cases of NCC and 2 of 25 (8% each in non-cysticercal CNS infection controls and healthy controls. The test showed a sensitivity of 56.25%, specificity of 92%, positive predictive value of 87.09%, and negative predictive value of 70.76%. Results of the present study shows that the Dot-ELISA as a simple test can be used in the field or poorly equipped laboratories for diagnosis of NCC .O objetivo do presente estudo foi estandardizar e avaliar o Dot-ELISA, um teste simples e rápido para detectar anticorpos de cisticercos no soro para diagnóstico da neurocisticercose (NCC. O antígeno usado no estudo foi um homogenizado completo de cistos de Cysticercus cellulosae obtidos de porcos infectados e marcados sobre a membrana de nitrocelulose. Os soros testados foram coletados de pacientes com NCC e os soros controle de pacientes com outras doenças e estudantes saudáveis e doadores e sangue do "Jawaharlal Institute of Postgraduate Medical Education and Research Hospital", em Pondicherry, durante o período de estudo de 2001 a 2003. Dot-Elisa detectou anticorpos em 14 de 25 (56% casos suspeitos de NCC, em 13 de 23 (56,5% em CT/MRI casos provados de NCC e em 2 de 25

  20. Utilidad de sangre almacenada en papel de filtro para estudios serologicos por ELISA de inhibicion Use of filter paper strips on an ELISA inhibition test for serologic studies on dengue

    Directory of Open Access Journals (Sweden)

    S. Vázquez

    1991-08-01

    Full Text Available Para evaluar la utilidad del método de recolección de muestras de sangre en papel de filtro para la detección de anticuerpos anti-dengue mediante un ELISA de Inhibición recientemente desarrollado en nuestro laboratorio, se realizó una toma simultánea de muestras de sangre en papel de filtro y de suero, de donantes de Banco de Sangre. Ambas muestras fueron conservadas a -20ºC y probadas a los 15 dias, 3 y 6 meses respectivamente. A las muestras que resultaron positivos se les amplió el rango de dilución para determinar título. Al realizar la comparación entre ambas muestras, sangre en papel de filtro y suero, encontramos que no existían diferencias de detección significativas, tanto para los casos positivos como los negativos. No obstante se observó en ambas muestras y de forma general una disminución del titulo de anticuerpos (una dilución al transcurrir el tiempo máximo establecido en nuestro estudio (6 meses.To evaluate the usefullness of the collecting method of blood samples in filter paper strips for anti-dengue antibody detection using an ELISA Inhibition test which has been recently developed in our laboratory, blood samples collected on filter paper strips and serum samples were obtained from the same blood donors. Both samples were kept at -20ºC and tested at 15 days, 3 and 6 months. Titers were determined in positive samples. Comparing the results, no significant differences were found between serum samples and blood samples collected in filter paper strips in relation to the number of negative and positive cases. Nevertheless, it was observed in both types of samples a decrease of the antibody titers (one dilution in the longest period of our study (6 months.

  1. The FAO/IAEA rinderpest indirect ELISA kit

    International Nuclear Information System (INIS)

    To meet the needs of the sero-monitoring component of the Pan African Rinderpest Campaign, the Joint FAO/IAEA Division in conjunction with the Pirbright Laboratories of the Institute for Animal Health, UK, developed an ELISA kit for the detection of antibodies to rinderpest virus in cattle. This kit was specifically designed to be used in the sort of conditions likely to be met in laboratories in Africa. The kit was based on an indirect ELISA and contained all the necessary reagents to test 20,000 cattle sera in duplicate. The kit also contained a detailed protocol (reproduced in this document) describing how to carry out and interpret the results of the test, a list of all the reagents and how to prepare and store them and information on the types of problems likely to be encountered in using the kit and how to solve them. The FAO/IAEA rinderpest ELISA kit has now been used to test over a quarter of a million cattle sera and has been shown to be a robust and reliable system for the sero-monitoring of rinderpest in Africa. (author). 7 refs, 2 figs

  2. Influence of affinity on antibody determination in microtiter ELISA systems

    International Nuclear Information System (INIS)

    Theoretically, all immunoassays are affinity (Ka) dependent when the product of the antibody (Ab) Ka and the free epitope concentration is less than 10. Thus, the degree of dependence on Ka depends on the concentration of available antigen in the system. The authors examined the binding of 125I-anti-fluorescein (a-FLU) monoclonal antibodies of different affinities to FLU-gelatin adsorbed on Immunlon 2 microtiter plates. Data obtained were in general agreement with our theoretical predictions; the percent of 125I-a-FLU which bound correlated with Ka, as did the shape of the titration curves. Measurement of 5 a-FLU monoclonals by the ELISA showed that the determination of Ab concentrations depends on the FLU-gelatin concentration, epitope density, and on the relationship between the Kas of test samples and the reference standard Ab preparation. Thus the ELISA is Ka dependent and should not be used routinely to estimate the absolute amount to Ab in unknown samples. However, the Ka dependency of the ELISA might provide a convenient assay for the estimation of the relative functional Ka (rfKa) of antibody preparations

  3. Estudio comparativo de un PCR anidado, ELISA y AGID en la detección del virus de la leucosis bovina en muestras de suero, sangre y leche Comparative study of nested PCR, ELISA and AGID tests in the detection of bovine leukaemia virus infection in serum, blood and milk samples

    Directory of Open Access Journals (Sweden)

    R Felmer

    2006-01-01

    Full Text Available Se evaluaron distintos métodos actualmente disponibles para el diagnóstico de la infección por el virus de la leucosis bovina (VLB. Los métodos empleados fueron AGID en suero, ELISA en muestras de suero y leche y PCR en linfocitos sanguíneos. De un total de 126 animales analizados, AGID identificó un menor número de animales positivos (75 comparado con las pruebas PCR y ELISA aplicadas en muestras de suero y leche (100. Tres animales positivos a AGID fueron negativos a PCR y 28 de las 51 muestras negativas a AGID fueron positivas mediante PCR. La sensibilidad diagnóstica de PCR con respecto a AGID fue de 96%, mientras que la especificidad fue de 45% (kappa 0,45. Todos los animales positivos a AGID fueron también positivos a ELISA aplicado tanto en suero como en leche, mientras que 25 animales negativos a AGID fueron consignados como positivos a ELISA, en ambas muestras biológicas. De esta forma, la sensibilidad diagnóstica de ELISA respecto a AGID fue de un 100%, mientras que la especificidad fue de 51% (kappa 0,55. La menor sensibilidad observada de AGID no es debido a reacciones falso positivas de ELISA y PCR, sino más bien a una mayor sensibilidad de estas últimas, lo que sugiere reconsiderar la utilización del método AGID en aquellos países en que aún se utiliza como método oficial en los programas de erradicación de leucosis.Different methods available for the detection of bovine leukaemia virus (BLV infection were evaluated. The methods evaluated were AGID in serum, ELISA in serum and milk, and PCR in blood lymphocytes. The AGID test identified a smaller number of positive animals (75/126 compared to PCR and ELISA tests (100/126. Three positive animals by AGID were negative by PCR and 28 of the 51 negative samples by AGID were positive by PCR. The sensitivity of PCR with respect to AGID was 96%, whereas the specificity was 45% (kappa 0.45. All positive animals by AGID were also positive by ELISA in serum and milk samples

  4. Televisión en Europa: análisis y comparativa de las principales cadenas públicas en seis países

    OpenAIRE

    Camáñez García, Gemma Cristina

    2005-01-01

    En España se vive un momento en el que se va a implantar una nueva definición del servicio público de televisión, y en el que se estudia la adjudicación de nuevas licencias de emisión a cadenas de titularidad privada. El sector de la televisión se caracteriza por estar en constante evolución. La ley de la oferta y la demanda es la moneda de cambio de este medio, y obliga a una renovación inmediata en cuanto a contenidos y tecnologías aplicadas (internet, telefonía móvil, digital, ...

  5. La cadena de frío vacunal en un departamento de salud de la Comunidad Valenciana The vaccine cold chain in a Valencian health department (Spain

    Directory of Open Access Journals (Sweden)

    Carmen Barber-Hueso

    2009-04-01

    Full Text Available Objetivo: Evaluar la cadena de frío en los puntos de vacunación de un departamento de salud de la Comunidad Valenciana, incluida la formación de los responsables de esta cadena. Método: Estudio transversal de revisión de un departamento de salud de la Comunidad Valenciana con 50 puntos de vacunación y 68 frigoríficos. Se utilizó una encuesta estructurada que recogía información relativa a las características del centro de vacunación, el personal responsable, la infraestructura y el control de los elementos que componen la cadena de frío, los procedimientos usados en la conservación y el manejo de las vacunas, así como los conocimientos del personal. Resultados: Se obtuvo una tasa de respuesta del 100%. Los frigoríficos eran, en su mayor parte, equipos domésticos sin alarma de avería o corte eléctrico (76,7%, alarma de puerta abierta (98,5%, termómetro exterior (92,6%, descongelación automática (76,5%, sensores internos de temperatura ni conexión a circuitos de emergencia (85,3%. La mayoría tenía la temperatura en el rango correcto (83,9%, las vacunas estaban adecuadamente situadas (88,2% y todos los centros contaban con un responsable de vacunas. En un 33,8% de las neveras había alimentos; en el 32,4% los viales se conservaban en la mesa durante la jornada vacunal y el gráfico de temperatura no se cumplimentaba diariamente (75%. Conclusión: Pese a que 5 de cada 6 neveras mantenían la temperatura adecuada, parece necesario mejorar la infraestructura vacunal de los centros de salud de atención primaria y la formación de los profesionales que intervienen en la cadena de frío.Objective: To evaluate the cold chain in the vaccination points of a health department in the autonomous region of Valencia, including training of the staff responsible for the cold chain. Method: We performed a crosssectional study to review all the vaccination points (n=50, with 68 refrigerators of a health department in the autonomous region

  6. Cadenas operativas y suelos de ocupación. El nivel 9 de la cueva de Las Caldas (Asturias, España

    Directory of Open Access Journals (Sweden)

    CORCHÓN, M.S., ORTEGA, P. y VICENTE, F.J.

    2013-01-01

    Full Text Available El amplio registro de puntas foliáceas procedentes de la Cueva de Las Caldas permite realizar un estudio pormenorizado de las cadenas operativas en el Solutrense superior. El objetivo perseguido es el conocimiento de las prácticas sociales que subyacen en la realización de estos útiles característicos por parte de los grupos de cazadores-recolectores solutrenses. Los procesos de talla implican la dispersión de desechos de talla característicos, que pueden ser reconocidos e identificados, como sucede en el nivel 9 de Las Caldas. Éste, identificado en la Sala I del yacimiento como un paleo-relieve con materiales in situ del Solutrense superior, constituye un escenario idóneo para este tipo de estudios.

  7. Metodología para la Evaluación del Rendimiento de la Cadena Logística Methodology for Evaluation of Performance of the Logistics Chain

    Directory of Open Access Journals (Sweden)

    J. Campos

    2004-01-01

    Full Text Available Este artículo presenta una metodología, denominada MESIADLog que permite construir un sistema de evaluación del rendimiento de la cadena logística integral, considerando diferentes aspectos no resueltos completamente por las metodologías existente. La metodología se ha aplicado a empresas españolas y brasileñas para validarla, constatando el potencial existente para el desarrollo de investigaciones en este campo y generar casos de uso que faciliten su difusión. Se muestran ejemplos prácticos de su aplicación en una empresa española del sector cerámico. Se concluye que la metodología propuesta permite a las empresas la elaboración de su propio sistema de evaluación de la cadena logística, desde un punto de vista integradoThis paper describes a methodology, called MESIADLog, which allows for the construction of an integrated system for the evaluation of supply chain performance that takes into account certain aspects that do not wholly fit into other existing methodologies. This methodology was applied to Spanish and Brazilian firms for its validation, determining the potential that exists for conducting research in this field and generating cases which can aid in its dissemination. In this paper practical examples of its application to a Spanish ceramics company are described. It is concluded that the proposed methodology permits companies to develop their own evaluation system for the logistic chain from an integrated perspective

  8. EVALUATION OF AN O-ANTIGEN ELISA FOR SCREENING CATTLE HERDS FOR SALMONELLA-TYPHIMURIUM

    DEFF Research Database (Denmark)

    Hoorfar, Jeffrey; Bitsch, V.

    1995-01-01

    dublin (0:1,9,12)-ELISA described previously appeared to be more serogroup D-specific. Thus, the 0:1,4,5,12-ELISA appears to be useful for detecting herd infections with S typhimurium, and positive reactions may be further discriminated by the serogroup D-specific ELISA....

  9. Comparison of DOT-ELISA and Standard-ELISA for Detection of the Vibrio cholerae Toxin in Culture Supernatants of Bacteria Isolated from Human and Environmental Samples.

    Science.gov (United States)

    Meza-Lucas, Antonio; Pérez-Villagómez, María-Fernanda; Martínez-López, José-Patricio; García-Rodea, Ricardo; Martínez-Castelán, María-Guadalupe; Escobar-Gutiérrez, Alejandro; de-la-Rosa-Arana, Jorge-Luis; Villanueva-Zamudio, Altagracia

    2016-09-01

    A comparison of DOT-ELISA and Standard-ELISA was made for detection of Vibrio cholerae toxin in culture supernatants of bacteria isolated from human and environmental samples. A total of 293 supernatants were tested in a double blind assay. A correlation of 100 % was obtained between both techniques. The cholera toxin was found in 20 Inaba and 3 Ogawa strains. Positive samples were from seafood (17 samples), potable water (1 sample) and sewage (5 samples). The DOT-ELISA was useful as the standard-ELISA to confirm the presence of cholera toxin in the environmental samples. PMID:27407304

  10. Obtención de extractos de membrana externa de Vibrio cholerae O1, mediante el uso de diferentes detergentes

    Directory of Open Access Journals (Sweden)

    José Luis Pérez

    2006-04-01

    Full Text Available En la actualidad existen dos variantes principales de vacunas orales contra el cólera: una basada en células inactivadas de diferentes biotipos y serotipos y otra basada en la administración de cepas vivas genéticamente atenuadas. Una vacuna por subunidades pudiera ser una variante muy atractiva. Este trabajo describe la purificación parcial y caracterización preliminar de extractos de proteínas de membrana externa-lipopolisacárido (PME-LPS, obtenidos a partir de Vibrio cholerae O1, con el interés de seleccionar un proteoliposoma que posteriormente será estructurado en forma de cocleatos para su uso por vía oral en humanos. Las preparaciones fueron obtenidas a través del uso de diferentes detergentes. La cantidad de LPS en cada preparación fue estimada mediante la determinación de las unidades endotóxicas en el ensayo del Limulus (LAL. La composición de cada muestra fue evaluada mediante SDS-PAGE y Dot Blot. La inoculación intranasal (IN en ratones Balb/c se utilizó para la evaluación de la inmunogenicidad de las preparaciones, y la respuesta inmune fue determinada por ELISA y el título de anticuerpos vibriocidas. El tamaño molecular de la preparación con mejores resultados en inmunogenicidad se estimó mediante la cromatografía en Sephacryl S-1000. Se obtuvieron diferentes perfiles electroforéticos de acuerdo con el tipo de detergente utilizado. El LPS fue identificado en todas las preparaciones y aquella obtenida con el SDS al 15% mostró la más baja relación proteínas/LPS y los mejores resultados en los ensayos de inmunogenicidad. Adicionalmente se comprobó que su tamaño molecular es similar al observado en el proteoliposoma de VAMENGOC- BC. La preparación obtenida con el SDS al 15% constituye un proteoliposoma, con capacidad para estimular altos niveles de anticuerpos IgG anti-LPS y altos títulos de anticuerpos vibriocidas, luego de su administración por vía intranasal en ratones. Estos resultados constituyen

  11. Control domótico remoto de vivienda mediante smartphone

    OpenAIRE

    ALEIXANDRE TUDO, BERNARDO

    2013-01-01

    La tecnología forma parte de la sociedad actual y de la vida cotidiana de las personas. Los últimos avances tecnológicos han simplificado nuestra vida y han provocado muchos cambios en las diversas áreas de la sociedad. Una de estas áreas es sin duda la automatización de la vivienda. Los nuevos aparatos electrónicos nos permiten tener acceso a las comunicaciones en cualquier lugar y a cualquier hora. La entrada de internet móvil en el mercado mediante los Smartphone y su ráp...

  12. Vaccination and ELISA: A partnership against Aujeszky's disease

    International Nuclear Information System (INIS)

    Aujeszky's disease is an important disease of swine. Vaccination is widely practised to control this disease. Although most vaccines offer clinical protection against the disease, they only reduce but do not prevent virus excretion or the establishment of latency after infection. Therefore vaccination alone will not lead to eradication of the disease. For this reason several serological test and elimination programmes have been developed. These programmes require either that pigs are not vaccinated or that they are vaccinated with vaccines that evoke an antibody response in serum that can be distinguished from the antibody response after infection. Differentiation between vaccinated and infected pigs is possible with the use of vaccine strains that lack one of the non-essential but highly immunogenic viral glycoproteins in combination with a glycoprotein specific antibody assay. Three such assays have been reported, based respectively on the demonstration of antibodies against the viral glycoproteins gI, gX and gIII. Because all field strains express gI and because most vaccines are gI negative, the presence of antibodies to gI seems the most logical indicator for infection with field virus. Several enzyme linked immunosorbent assay (ELISA) techniques have been developed to detect antibodies to gI, based either on the principle of a blocking ELISA or on the principle of an indirect ELISA, using affinity purified gI as antigen coated to the wells of microtitre plates. The antibody response against gI after infection of vaccinated and non-vaccinated pigs has been examined in detail. Infected pigs not vaccinated or vaccinated with a gI negative vaccine develop antibodies against gI which appear to persist for more than two years. Even pigs with maternal antibodies deficient in antibodies to gI that become infected with low doses of mildly virulent Aujeszky's disease virus develop antibodies to gI, albeit at low titres. (author). 51 refs, 1 fig

  13. Development of ELISA and immunochromatographic assay for ofloxacin

    Institute of Scientific and Technical Information of China (English)

    Wu Yong Sun; Wen Ying Liu; Ling Bo Qu

    2007-01-01

    Two rapid, sensitive and reliable immunoassay methods, namely competitive indirect enzyme-linked immunosorbent assay (CIELISA) and colloidal gold-based immunochromatographic assay (CGIA), were developed to detect ofloxacin (OFL). The linear range of the CI-ELISA was from 0.5 to 128 ng/mL with a limit of detection (LOD) of 0.35 ng/mL. Good recoveries were obtained in analyzing simulated swine urine samples. The CGIA could accurately estimate OFL at concentrations as low as 10 ng/mL in less than 10 min, and test results were read visually without any instrument.

  14. The ELisA Facility - RESTful API and Client Libraries

    CERN Document Server

    Corso-Radu, A; The ATLAS collaboration; Murillo Garcia, R

    2013-01-01

    The ATLAS experiment at the LHC (CERN) comprises a large and geographically distributed community of over three thousand scientists from all over the world. Data acquisition is supervised by a shift crew of about 10 people running the experiment 24/7. Information concerning the experiment operation, configuration and behavior has to be reported, gathered and shared with the whole community. To provide such functionality, a logbook facility tool, known as ELisA, has been developed to offer an user-friendly web interface to browse activity logs and an effective way for shifters and experts to report on system operations.\

  15. Detection of antibodies to the 20s proteasome by ELISA

    DEFF Research Database (Denmark)

    Jørgensen, Karin Meinike; Frederiksen, Jette Lautrup; Nielsen, Christoffer Tandrup;

    2013-01-01

    The presence of antibodies against the 20S proteasome has been correlated with diseases like multiple sclerosis (MS) and systemic lupus erythematosus (SLE) but no definite association has been established. In order to investigate this further, we optimized an ELISA for proteasome antibodies...... and applied it to test a total of 324 serum and plasma samples from MS patients, SLE patients, and healthy controls. Our results yield a functional and reliable assay but no correlation between the amount of proteasome antibodies present and the development of MS or SLE could be established....

  16. en sistemas modelo de gelatina mediante SPME-DED

    Directory of Open Access Journals (Sweden)

    J. Ruiz Carrascal

    2005-01-01

    Full Text Available Se llevó a cabo la determinación de 16 hidrocarburos aromáticos policíclicos y 9 nitrosaminas en sistemas modelo de gelatina (20% w/v mediante microextración en fase sólida acoplada a un dispositivo de extracción directa y posterior análisis mediante cromatografía-gaseosa-espectrometría de masas. La extracción se llevo a cabo a 25ºC. Se extrajeron la totalidad de las nitrosaminas y 9 de los 16 hidrocarburos aromáticos policíclicos. Se evaluaron la reproducibilidad, linealidad de respuesta y límite de detección de 3 tipos de fibras estacionarias para cada tipo de compuesto. Se seleccionaron las fibras de polidimetilsiloxano 100μm y carboxen/polidimetilsiloxano 85μm, para los hidrocarburos aromáticos policiclicos y nitrosaminas, respectivamente. La microextración en fase sólida acoplada a un dispositivo de extracción directa surge como una técnica interesante para la monitorización preliminar de la presencia de estos compuestos tóxicos en alimentos sólidos, sin necesidad de toma de muestras y sin deteriorar el producto

  17. ELISA, a demonstrator environment for information systems architecture design

    Science.gov (United States)

    Panem, Chantal

    1994-01-01

    This paper describes an approach of reusability of software engineering technology in the area of ground space system design. System engineers have lots of needs similar to software developers: sharing of a common data base, capitalization of knowledge, definition of a common design process, communication between different technical domains. Moreover system designers need to simulate dynamically their system as early as possible. Software development environments, methods and tools now become operational and widely used. Their architecture is based on a unique object base, a set of common management services and they host a family of tools for each life cycle activity. In late '92, CNES decided to develop a demonstrative software environment supporting some system activities. The design of ground space data processing systems was chosen as the application domain. ELISA (Integrated Software Environment for Architectures Specification) was specified as a 'demonstrator', i.e. a sufficient basis for demonstrations, evaluation and future operational enhancements. A process with three phases was implemented: system requirements definition, design of system architectures models, and selection of physical architectures. Each phase is composed of several activities that can be performed in parallel, with the provision of Commercial Off the Shelves Tools. ELISA has been delivered to CNES in January 94, currently used for demonstrations and evaluations on real projects (e.g. SPOT4 Satellite Control Center). It is on the way of new evolutions.

  18. Development of a microplate reader compatible microfluidic chip for ELISA.

    Science.gov (United States)

    Hou, Fenghua; Zhang, Qin; Yang, Jianping; Li, Xinchun; Yang, Xiujuan; Wang, Shuping; Cheng, Zhiyi

    2012-08-01

    We report a novel microfluidic device use for sandwich enzyme-linked immunoassay assay (ELISA). The related procedures including the introduction of reagents, dilution and distribution of samples, as well as immobilization of enzyme can be readily carried out on a poly (dimethylsiloxane) (PDMS) chip. Particularly, this microfluidic chip comprising of two distinct parallel units, and has an identical dimension as a conventional microtiter plate, which offers access to the directly quantitative detection by the microplate reader. Gradient-concentration reacting solutions at six different concentrations level generated by the microfluidic channel network are simultaneously transported to 24 reaction chambers to form enzymatic products. Alkaline phosphatase (ALP), 4-methylumbelliferyl phosphate (4-MUP) and KH(2)PO(4) are used as enzyme-substrate-inhibitor model, to demonstrate the utility of the developed microchip-based enzyme inhibitor assay. Various conditions such as the surface treatment of chip channels, fluids velocities, substrate concentration, and buffer pH are investigated. The present microfluidic device for ELISA holds several advantages, for instance frugal usage of samples and reagents, less of operating time, favorably integrated configuration, ease of manipulation, and could be explored to a variety of high throughput drug screening. PMID:22526682

  19. eLISA: Astrophysics and cosmology in the millihertz regime

    CERN Document Server

    Amaro-Seoane, Pau; Babak, Stanislav; Binétruy, Pierre; Berti, Emanuele; Bohé, Alejandro; Caprini, Chiara; Colpi, Monica; Cornish, Neil J; Danzmann, Karsten; Dufaux, Jean-François; Gair, Jonathan; Jennrich, Oliver; Jetzer, Philippe; Klein, Antoine; Lang, Ryan N; Lobo, Alberto; Littenberg, Tyson; McWilliams, Sean T; Nelemans, Gijs; Petiteau, Antoine; Porter, Edward K; Schutz, Bernard F; Sesana, Alberto; Stebbins, Robin; Sumner, Tim; Vallisneri, Michele; Vitale, Stefano; Volonteri, Marta; Ward, Henry

    2012-01-01

    This document introduces the exciting and fundamentally new science and astronomy that the European New Gravitational Wave Observatory (NGO) mission (derived from the previous LISA proposal) will deliver. The mission (which we will refer to by its informal name "eLISA") will survey for the first time the low-frequency gravitational wave band (about 0.1 mHz to 1 Hz), with sufficient sensitivity to detect interesting individual astrophysical sources out to z = 15. The eLISA mission will discover and study a variety of cosmic events and systems with high sensitivity: coalescences of massive black holes binaries, brought together by galaxy mergers; mergers of earlier, less-massive black holes during the epoch of hierarchical galaxy and black-hole growth; stellar-mass black holes and compact stars in orbits just skimming the horizons of massive black holes in galactic nuclei of the present era; extremely compact white dwarf binaries in our Galaxy, a rich source of information about binary evolution and about futur...

  20. HIV serology using fourth-generation ELISA techniques

    Directory of Open Access Journals (Sweden)

    Aline Daniele Schuster

    2014-04-01

    Full Text Available Backgound and Objectives: Since its introduction in the mid-1980s, HIV testing has gradually improved in terms of sensitivity and specificity. A study was carried out to assess the techniques used in a hemotherapy center for human immunodeficiency virus (HIV identification using two 4th-generation enzyme-linked immunosorbent assay (ELISA methods. Methods: descriptive and retrospective study of 4th-generation ELISA techniques, which analyzed all the results generated from blood samples obtained from donors aged 16 to 68 years, according to current legislation, who attended a regional blood bank from November 2010 to October 2011. Results: Of 8,475 samples collected through blood donation, 06 were positive or inconclusive at the screening tests and were then analyzed through Western Blot confirmatory test, which confirmed one of them was reagent for the virus. Conclusion: Several factors influence positive results of screening samples, such as type of test used, the immunological window, use of antiretroviral drugs, and personal biological factors of infected individuals. The effectiveness of HIV screening tests should be determined and the methodology improved, so that it can establish with certainty the existence or not of contamination of blood products that will be used later. More research is needed to constantly improve the screening tests and give adequate emphasis to factors that have shown to alter results.

  1. El frágil inicio de la cadena de frío vacunal en España The fragile beginning of the vaccine cold chain in Spain

    OpenAIRE

    José Tuells

    2010-01-01

    La realización de dos estudios de ámbito nacional durante 1988-89 con el objetivo de analizar la situación de la cadena de frío vacunal en España activó el interés por la logística de las vacunas entre los profesionales de la salud pública. Se realizaron utilizando la metodología de evaluación basada en tarjetas de monitorización tiempo-temperatura (informe Battersby) y encuestas transversales sobre el estado de la cadena de frío vacunal (informe Ferrando) en los niveles provincial y local (2...

  2. LA ELECCIÓN DEL MODO DE ENTRADA EN UN MERCADO INTERNACIONAL. EL CASO DE LAS CADENAS HOTELERAS DE BALEARES EN LA REGIÓN DEL CARIBE Y EL GOLFO DE MÉXICO

    Directory of Open Access Journals (Sweden)

    Martorell Cunill, O.

    2010-01-01

    Full Text Available Esta investigación trata de determinar los factores clave que influyen en el proceso de decisión llevado a cabo por las cadenas hoteleras de baleares a la hora de elegir su estrategia de crecimiento para llevar a cabo su expansión en la región del Caribe y el golfo de México con la finalidad de verificar si los conceptos derivados del coste de transacción, teoría de la agencia y la teoría estratégica de la organización de habilidades y conocimiento de las empresas pueden explicar la modalidad de expansión elegida por estas cadenas en dicha región. Este trabajo permite la comparación de los resultados obtenidos con los obtenidos por otros trabajos similares sobre la industria hotelera internacional.

  3. Impacto del uso de las tecnologías de la información y la comunicación que apoyan la cadena de suministro (TIC-CS sobre el desempeño organizacional

    Directory of Open Access Journals (Sweden)

    Víctor Andrés Peñates Montes

    2015-10-01

    Full Text Available Este trabajo, por medio de una revisión de la literatura relevante, pretende determinar y analizar la naturaleza y la forma que tienen los impactos sobre el desempeño organizacional generados por el uso de las tecnologías de la información y la comunicación que apoyan la cadena de suministro (TIC-CS. Primero se realiza un breve esbozo de los conceptos de desempeño y de cadena de suministro, luego se revisan y discuten los resultados de veintiséis investigaciones empíricas relacionadas con dicho impacto. Se identifica que estas investigaciones reportan impactos de naturaleza positiva en cuatro formas diferentes. Se sugiere, finalmente, actuar usando complementariedades para disminuir los riesgos asociados a la eficacia del proceso de implementación de las TIC-CS.

  4. SIMPLIFIED DIAGNOSIS OF MALARIA INFECTION: GFM/PCR/ELISA A SIMPLIFIED NUCLEIC ACID AMPLIFICATION TECHNIQUE BY PCR/ELISA

    Directory of Open Access Journals (Sweden)

    Ricardo Luiz Dantas MACHADO

    1998-09-01

    Full Text Available We report an adaptation of a technique for the blood sample collection (GFM as well as for the extraction and amplification of Plasmodium DNA for the diagnosis of malaria infection by the PCR/ELISA. The method of blood sample collection requires less expertise and saves both time and money, thus reducing the cost by more than half. The material is also suitable for genetic analysis in either fresh or stored specimens prepared by this method.Relatamos a adaptação de uma técnica para coleta de amostras (MFV e outra para extração, amplificação de DNA de parasitas da malária para diagnóstico por PCR/ELISA. O método de coleta de amostras requer menos habilidade e economisa tempo e dinheiro, assim reduzindo a mais da metade o custo. O material é também adequado para análise genética em especimens frescos ou estocados, preparados por este método.

  5. Prueba de Elisa indirecta para la detección de anticuerpos IgM para el diagnóstico de Leptospirosis humana

    Directory of Open Access Journals (Sweden)

    Manuel Céspedes Z

    2002-01-01

    Full Text Available Para el diagnóstico temprano de enfermedades con cuadro clínico inespecífico como la leptospirosis, es necesario la confirmación laboratorial mediante pruebas específicas, con la finalidad de que el diagnóstico sea más acertado y rápido. Objetivo: se realizó un estudio comparativo entre la prueba de microaglutinacion (MAT y la prueba de ELISA indirecta estandarizada con un "pool" de antígenos de Leptospira interrogans, para la detección de anticuerpos IgM, en muestras de suero de fase aguda de leptospirosis humana. Materiales y métodos: 40 muestras de pacientes con sospecha clínica y con títulos de 1:100-1:12800 por la prueba de MAT, 80 muestras negativas de pacientes aparentemente sanos con enfermedades como Brucelosis, Sífilis, Tifus murino, Hepatitis B, Fiebre Amarilla, Dengue y Enfermedad de Carrión fueron evaluados por ELISA IgM. Resultados: se obtuvo una sensibilidad de 97,5% y especificidad de 98,75%, no observándose reacción cruzada con otras enfermedades. Conclusión: ELISA IgM validado en el laboratorio es suficiente sensible, específico y de fácil aplicación para el uso como prueba de tamizaje en una infección por Leptospiras con la subsecuente confirmación por MAT.

  6. Estructura y función de la cadena productiva de carne de bovino en la ganadería ejidal de Tecpatán, Chiapas, México

    Directory of Open Access Journals (Sweden)

    J. Calderón

    2012-01-01

    Full Text Available El objetivo de la presente investigación fue analizar la estructura y el funcionamiento de la cadena productiva de carne bovina en el municipio de Tecpatán, Chiapas, México. Se aporta información cualitativa y cuantitativa orientada a comprender y consolidar las alianzas entre actores de la cadena productiva. Los resultados muestran la existencia de los eslabones de producción de bovinos en pie, comercialización, transformación y venta de carne. Los servicios de apoyo están dados por tres asociaciones ganaderas (ag, dos rastros municipales y un rastro tipo inspección federal. Existen cuatro circuitos de comercialización bien diferenciados en cuanto a número y tipo de actores que participan en la relación de producción- compra-venta, producto comercializado y mercado final de destino. El circuito que involucra mayor cantidad de animales en pie es el que comercializa becerros destetados para engorda. En todos los circuitos, el mayor margen de comercialización lo obtienen los intermedia-rios que participan en el eslabón de comercialización. Los beneficiarios son principalmente acopiadores mayoristas regionales y nacionales del circuito más corto. En el aspecto organizacional, los productores cuentan con AG locales y regionales, mientras que los agentes de la comercialización no cuentan con ningún tipo de organización. Se requiere una política de apoyo permanente para consolidar alianzas entre los actores, así como una estrategia de competitividad orientada a diferenciar los productos. De esta forma, la cadena productiva actual podría transitar poco a poco hacia una cadena de valor.

  7. Optimización de la cadena de suministro: Implantación de un sistema SMI( Supplier Managed Inventory) en una empresa de fabricación de bebidas alcohólicas

    OpenAIRE

    Montero Ortiz, Roberto

    2015-01-01

    La elevada exigencia competitiva en el mundo empresarial es el principal motor del cambio. Para tener éxito en un entorno tan agresivo, es necesario llevar a la empresa al siguiente nivel. No es suficiente con integrar todos los actores internos dentro de una cadena eficiente; la clave está en los externos. Una vez hemos optimizado nuestros procesos internos, debemos poner el foco en todos aquellos agentes externos que escapan a nuestro control directo. Es necesario cambiar de ...

  8. ELISA Detection of Francisella tularensis using Polyclonaland Monoclonal Antibodies

    Directory of Open Access Journals (Sweden)

    Miroslav Pohanka

    2008-09-01

    Full Text Available The mouse monoclonal and polyclonal antibodies were produced for the detection of intracellular pathogenand potential warfare agent Francisella tularensis. Antibody titers obtained were 1:640 for polyclonal antibodiesand 1:320 for monoclonal antibodies. Both antibodies were used in the indirect enzyme-linked immunosorbentassay (ELISA found to detect F. tularensis whole cells. The limit of detection was 5.4×106 CFU/ml for polyclonalantibodies and 6.9×106 CFU/ml for monoclonal antibodies. The value sample could  be distinguished from anyconcentration of another gram-negative bacterium: Escherichia coli.Defence Science Journal, 2008, 58(5, pp.698-702, DOI:http://dx.doi.org/10.14429/dsj.58.1693

  9. [ELISA method for the determination of factor VII antigen].

    Science.gov (United States)

    Jorquera, J I; Aznar, J A; Monteagudo, J; Montoro, J M; Casaña, P; Pascual, I; Bañuls, E; Curats, R; Llopis, F

    1989-12-01

    The low plasma concentration of clotting factor VII makes it difficult to assay its antigenic fraction by the conventional methods of precipitation with specific antigens. Simple and peroxidase-conjugated antisera are currently available from commercial sources, thus allowing one to determine F VII:Ag by enzyme immunoassay. An ELISA method has been developed in this laboratory which provides sensitivity limits about 0.1% of the plasma concentration of F VII and correlates significantly with its functional activity (r = 0.603, n = 44, p less than 0.001). This technique can be highly helpful in characterising molecular variants of F VII, as well as in detecting acquired deficiencies of this factor.

  10. Las cadenas léxicas como exponentes de coherencia textual en la pragmática escrita de la interlingua

    Directory of Open Access Journals (Sweden)

    Francisca Suau Jiménez

    2011-04-01

    Full Text Available Normal 0 21 false false false ES X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} La pragmática de la interlingua, aunque de reciente aparición dentro de la teoría que explica la adquisición de una L2, ha demostrado que permite analizar el comportamiento pragmalingüítico de los hablantes no-nativos de una lengua de modo más preciso que otras aproximaciones sociopragmáticas. A través de los resultados de una investigación empírica pretendemos contribuir a esta línea de trabajo aportando las conclusiones de un estudio realizado con hablantes nativos (HN y no-nativos (HNN. A partir del estudio comparativo de las cadenas de cohesión léxica que los dos grupos de sujetos utilizan en la construcción de la coherencia textual pretendemos averiguar si la competencia retórica de los HNN seria paralela a su competencia gramatical y, por lo tanto, inferior a la de los HN, o si, por el contrario, no es así, debido a la posible transferencia de patrones retóricos de la LI en los HNN. Para ello hemos aplicado el modelo de redes de cadenas léxicas de Hoey (1991. Los resultados del trabajo sugieren que arribas competencias, la gramatical y la retórica no contemplan paralelismo alguno en el uso que hacen ambos

  11. Evaluation of an indirect ELISA for detection and typing of foot-and-mouth disease virus

    International Nuclear Information System (INIS)

    An indirect enzyme linked immunosorbent assay (ELISA) kit was used for diagnosis of foot-and-mouth disease virus (FMDV) types O1, A23, C3 which occurred in Rio Grande do Sul State, Southern Brazil during 1984-1994. The samples were randomly selected and tested by ELISA, Complement Fixation Test (CFT) and in tissue culture. Out of 106 samples 78 (73,5%) were positive by ELISA and 39 (36,8%) were found positive in CFT, when original suspensions were used. Once these samples were inoculated onto tissue culture both tests gave similar results, although ELISA picked up more positive samples during the 1st passage in tissue culture. The negative samples (16) included in this study were negative in all tests. The ELISA was more sensitive than and as specific as CFT. ELISA and tissue culture together were shown to be a better system for detection of foot-and-mouth disease virus antigen than CFT. (author)

  12. Development and Comparative Evaluation of a Competitive ELISA with Rose Bengal Test and a Commercial Indirect ELISA for Serological Diagnosis of Brucellosis

    OpenAIRE

    Mythili, Tadepalli; Rajendra, Lingala; Bhavesh, Trangadia; Thiagarajan, Dorairajan; Srinivasan, Villuppanoor Alwar

    2011-01-01

    The development of a competitive ELISA for the detection of brucella-specific antibodies in bovines is described. Anti-brucella guinea pig serum was used as a source of competing antibodies. Lipo-polysaccharide purified from inactivated B. abortus S19 culture was used as antigen for the development of the assay. Sera from cattle were used in the competitive ELISA, rose bengal test and a commercial indirect ELISA. The following cattle sera were tested: (i) known positive sera (n = 80) (ii) kno...

  13. Parametric analysis of a novel semi-circular microfluidic CD-ELISA valve

    OpenAIRE

    Lin Samuel

    2011-01-01

    Abstract CD-ELISA uses the microfluidic ranking method and centrifugal force to control the testing solution as it flows into the reaction region. The most challenging part of CD-ELISA is controlling the flow process for different biological testing solutions, i.e. the controlling sequence for the microfluidic channel valves. The microfluidic channel valve is therefore the most important fluid channel structure for CD-ELISA. In this study, we propose a valve design suitable for a wide range r...

  14. Reactivity in ELISA with DNA-loaded nucleosomes in patients with proliferative lupus nephritis.

    Science.gov (United States)

    Dieker, Jürgen; Schlumberger, Wolfgang; McHugh, Neil; Hamann, Philip; van der Vlag, Johan; Berden, Jo H

    2015-11-01

    Autoantibodies against nucleosomes are considered a hallmark of systemic lupus erythematosus (SLE). We compared in patients with proliferative lupus nephritis the diagnostic usefulness of a dsDNA-loaded nucleosome ELISA (anti-dsDNA-NcX) with ELISAs in which dsDNA or nucleosomes alone were coated. First, we analysed whether DNA loading on nucleosomes led to masking of epitopes by using defined monoclonal anti-DNA, anti-histone and nucleosome-specific autoantibodies to evaluate the accessibility of nucleosomal epitopes in the anti-dsDNA-NcX ELISA. Second, autoantibody levels were measured in these 3 ELISAs in 100 patients with proliferative lupus nephritis (LN) before immunosuppressive treatment and in 128 non-SLE disease controls. In patients with LN inter-assay comparisons and associations with clinical and serological parameters were analysed. The panel of monoclonal antibodies revealed that all epitopes were equally accessible in the anti-dsDNA-NcX ELISA as in the two other ELISAs. Patients with proliferative lupus nephritis were positive with dsDNA-loaded nucleosomes in 86%, with DNA in 66% and with nucleosomes in 85%. In the non-lupus disease control group these frequencies were 1.6% (2 out of 128) for both the anti-dsDNA-NcX and the anti-dsDNA ELISA and 0% in the anti-nucleosome ELISA. The levels in the anti-dsDNA-NcX ELISA were high in a group of patients with LN that showed absent reactivity in the anti-DNA or low levels in the anti-nucleosome ELISA. Anti-dsDNA-NcX positivity was associated with higher SLEDAI scores within this group. Within nucleosome-based ELISAs, we propose the anti-dsDNA-NcX ELISA as the preferred test system. PMID:26597199

  15. Reactivity in ELISA with DNA-loaded nucleosomes in patients with proliferative lupus nephritis.

    Science.gov (United States)

    Dieker, Jürgen; Schlumberger, Wolfgang; McHugh, Neil; Hamann, Philip; van der Vlag, Johan; Berden, Jo H

    2015-11-01

    Autoantibodies against nucleosomes are considered a hallmark of systemic lupus erythematosus (SLE). We compared in patients with proliferative lupus nephritis the diagnostic usefulness of a dsDNA-loaded nucleosome ELISA (anti-dsDNA-NcX) with ELISAs in which dsDNA or nucleosomes alone were coated. First, we analysed whether DNA loading on nucleosomes led to masking of epitopes by using defined monoclonal anti-DNA, anti-histone and nucleosome-specific autoantibodies to evaluate the accessibility of nucleosomal epitopes in the anti-dsDNA-NcX ELISA. Second, autoantibody levels were measured in these 3 ELISAs in 100 patients with proliferative lupus nephritis (LN) before immunosuppressive treatment and in 128 non-SLE disease controls. In patients with LN inter-assay comparisons and associations with clinical and serological parameters were analysed. The panel of monoclonal antibodies revealed that all epitopes were equally accessible in the anti-dsDNA-NcX ELISA as in the two other ELISAs. Patients with proliferative lupus nephritis were positive with dsDNA-loaded nucleosomes in 86%, with DNA in 66% and with nucleosomes in 85%. In the non-lupus disease control group these frequencies were 1.6% (2 out of 128) for both the anti-dsDNA-NcX and the anti-dsDNA ELISA and 0% in the anti-nucleosome ELISA. The levels in the anti-dsDNA-NcX ELISA were high in a group of patients with LN that showed absent reactivity in the anti-DNA or low levels in the anti-nucleosome ELISA. Anti-dsDNA-NcX positivity was associated with higher SLEDAI scores within this group. Within nucleosome-based ELISAs, we propose the anti-dsDNA-NcX ELISA as the preferred test system.

  16. A multi-purpose ultrasonic streaming mixer for integrated magnetic bead ELISAs

    Science.gov (United States)

    Brandhoff, Lukas; Zirath, Helene; Salas, Mariugenia; Haller, Anna; Peham, Johannes; Wiesinger-Mayr, Herbert; Spittler, Andreas; Schnetz, Guntram; Lang, Walter; Vellekoop, Michael J.

    2015-10-01

    We present an ultrasonic streaming mixer for disposable and on-chip magnetic bead ELISAs. The ultrasonic transducer is placed at system-level to keep cost per chip as low as possible, and is coupled to the chip by means of a solid ultrasonic horn. The system provides mixing of liquids, as well as dispersion of the superparamagnetic beads in the ELISA. Additionally it can be used clean the chamber surface from nonspecifically bound proteins during the washing steps in the ELISA protocol. Using our system the time for the ELISA protocol has been greatly reduced down to 30 min.

  17. Sensitive detection of Escherichia coli O157:H7 based on cascade signal amplification in ELISA.

    Science.gov (United States)

    Shan, Shan; Liu, Daofeng; Guo, Qi; Wu, Songsong; Chen, Rui; Luo, Kai; Hu, Liming; Xiong, Yonghua; Lai, Weihua

    2016-09-01

    In this study, cascade signal amplification in ELISA involving double-antibody sandwich ELISA and indirectly competitive ELISA was established to sensitively detect Escherichia coli O157:H7. In the double-antibody sandwich ELISA, a complex was formed comprising anti-E. coli O157:H7 polyclonal antibody, E. coli O157:H7, biotinylated anti-E. coli O157:H7 monoclonal antibody, streptavidin, and biotinylated β-lactamase. Penicillin solution was then added into the ELISA well and hydrolyzed by β-lactamase. Afterward, the penicillin solution was transferred to indirectly competitive ELISA. The concentration of penicillin can be sensitively detected in indirectly competitive ELISA. In the cascade signal amplification system, increasing the amount of added E. coli O157:H7 resulted in more β-lactamase and less penicillin. The detection sensitivity of E. coli O157:H7, which was 20cfu/mL with the cascade signal amplification in ELISA, was 1,000-fold higher than that of traditional ELISA. Furthermore, the novel method can be used to detect E. coli O157:H7 in milk (2cfu/g). Therefore, this new signaling strategy will facilitate analyses of highly sensitive foodborne pathogens. PMID:27394946

  18. Una aproximación a una cadena de valor textil artesanal en el Noroeste de la Provincia de Córdoba

    Directory of Open Access Journals (Sweden)

    Martínez, Carlos Eduardo

    2011-01-01

    Full Text Available En el presente trabajo daremos cuenta del desarrollo de una cadena de valor textil artesanal que se realiza en el marco del trabajo de extensión de una agencia del Estado Argentino. En primer lugar y para ubicar contextualmente la experiencia analizaremos el contexto de cambios políticos y económicos acaecidos en nuestro País en las últimas décadas. Esta primera aproximación nos servirá de base para luego analizar los aspectos teóricos que conlleva la experiencia productiva y desde allí analizar desde una perspectiva antropológica los aspectos constitutivos de las unidades domésticas involucradas en el proyecto.In this paper we will realize the development of handicraft textile value chain is performed under the extension work of the Argentine State agency. First, and experience to locate contextually analyze the context of political and economic changes occurring in our country in recent decades. This first approach will serve as a base and then to analyze the theoretical aspects involved in production experience and from there from an anthropological perspective to analyze the constitutional aspects of the households involved in the project.

  19. Un estudio al impacto de las iniciativas de mejora de las cadenas de suministro y de fabricación en empresas que aplican MTO y MTS

    Directory of Open Access Journals (Sweden)

    Ariel L. Lituve

    2011-12-01

    Full Text Available Las empresas manufactureras tienen como objetivo mejorar los procesos internos y externos para incrementar la ventaja competitiva. Estos procesos incluyen prácticas de manufactura esbelta, de racionalización de proveedores y de integración logística. En este trabajo, se analizan estas prácticas y su impacto en el desempeño del negocio y, en particular, se exploran las diferencias entre las empresas que aplican make-to-order –MTO– y make-to-stock –MTS–, con los datos recolectados de 216 empresas manufactureras de Australia. En los resultados se encontró una clara diferencia del enfoque de mejora entre las empresas MTO y las MTS. Las MTO muestran un impacto significativo de la integración logística en el desempeño empresarial, pero no de las prácticas de manufactura esbelta y de racionalización de proveedores. La situación se revierte completamente para las empresas MTS que, aunque logran efectos significativos en las prácticas internas de manufactura esbelta y en la racionalización de proveedores, no lo logran en la integración logística. Además, que la distinción entre empresas MTO y MTS es importante cuando se analizan las iniciativas para mejorar las cadenas de fabricación y de suministro.

  20. Caracterización molecular de la cadena gama común y Jak3 en un individuo afectado con inmunodeficiencia severa combinada

    Directory of Open Access Journals (Sweden)

    Pablo Javier Patiño Grajales

    2001-04-01

    Full Text Available

    La Inmunodeficiencia Severa Combinada (IDSC es una enfermedad
    de origen genético, que se puede heredar de forma autosómica
    recesiva o ligada al cromosoma X. La IDSC se caracteriza por un
    defecto en el número y la diferenciación de los linfocitos T y NK. Los
    individuos afectados desarrollan diarrea crónica, infecciones persistentes y severas como neumonía, septicemia e infecciones fúngicas.
    Estos pacientes presentan retardo en el crecimiento y pueden morir a
    temprana edad si no se realiza una terapia de corrección genética o un
    trasplante de células hematopoyéticas. Las mutaciones responsables
    de la IDSC comprometen principalmente el gen de la cadena gama
    común (γc y la proteína Jak3 que son proteínas fundamentales en la
    transducción de señales de los receptores para varias citoquinas esenciales en la diferenciación y activación de células del sistema inmune, las cuales incluyen IL-2, IL-4, IL-7, IL-9 e IL-15 (1,2.

     

     

  1. MODELIZACIÓN DE LOS INDICADORES DE GESTIÓN EN LA CADENA DE SUMINISTRO. UNA VISIÓN SISTÉMICA

    Directory of Open Access Journals (Sweden)

    MARTÍN DARIO ARANGO SERNA

    2008-01-01

    Full Text Available El modelo que describe este artículo, pretende medir, analizar, evaluar y plantear un mejoramiento de los procesos administrativos, identificando las diferentes variables que intervienen en la gestión de la cadena de suministro centrándose en el subsistema de producción. Este trabajo hace parte de una investigación empírica que se viene desarrollando en el sector textil. El modelo muestra de forma empírica las relaciones que existen entre, las funciones internas de la empresa en el área de la producción y su habilidad para la creación de indicadores de gestión de la producción y la dinámica empresarial como camino al mejoramiento e innovación de procesos. Finalmente, se muestran algunas conclusiones relevantes obtenidas en las dos primeras fases de la investigación empírica, ejecutadas hasta el momento.

  2. Measuring hordein (gluten in beer--a comparison of ELISA and mass spectrometry.

    Directory of Open Access Journals (Sweden)

    Gregory J Tanner

    Full Text Available BACKGROUND: Subjects suffering from coeliac disease, gluten allergy/intolerance must adopt a lifelong avoidance of gluten. Beer contains trace levels of hordeins (gluten which are too high to be safely consumed by most coeliacs. Accurate measurement of trace hordeins by ELISA is problematic. METHODS: We have compared hordein levels in sixty beers, by sandwich ELISA, with the level determined using multiple reaction monitoring mass spectrometry (MRM-MS. RESULTS: Hordein levels measured by ELISA varied by four orders of magnitude, from zero (for known gluten-free beers to 47,000 µg/mL (ppm; for a wheat-based beer. Half the commercial gluten-free beers were free of hordein by MS and ELISA. Two gluten-free and two low-gluten beers had zero ELISA readings, but contained significant hordein levels (p<0.05, or near average (60-140% hordein levels, by MS, respectively. Six beers gave false negatives, with zero ELISA readings but near average hordein content by MS. Approximately 20% of commercial beers had ELISA readings less than 1 ppm, but a near average hordein content by MS. Several barley beers also contained undeclared wheat proteins. CONCLUSIONS: ELISA results did not correlate with the relative content of hordein peptides determined by MS, with all barley based beers containing hordein. We suggest that mass spectrometry is more reliable than ELISA, as ELISA enumerates only the concentration of particular amino-acid epitopes; this may vary between different hordeins and may not be related to the absolute hordein concentration. MS quantification is undertaken using peptides that are specific and unique, enabling the quantification of individual hordein isoforms. This outlines the problem of relying solely on ELISA determination of gluten in beverages such as beer and highlights the need for the development of new sensitive and selective quantitative assay such as MS.

  3. Serodiagnosis of human hydatidosis with an ELISA developed based on antigens derived from sheep hydatid cysts and comparison with a commercial human ELISA kit

    Institute of Scientific and Technical Information of China (English)

    Fotoohi S; Hashemi Tabar G.R; Borji H

    2013-01-01

    Objective: To explore the serodiagnosis of hydatid cyst in human using different antigens of sheep (hydatid fluid, Somatic and Excretory/secretory antigens of protoscolex) by ELISA and compares this result with commercial human ELISA kit. Methods: One hundred blood samples from patients with history of severe abdominal pain and eosinophilia were obtained. Ten serum samples were obtained from surgically and pathologically confirmed cystic echinococcosis patients from Mashhad university hospital as positive control and 5 serum samples from infant under one year old as negative control. Blood samples were centrifuged at 3 000íg at 20 ℃ for 15 min and sera were stored at -20 ℃. First, these samples were tested for the presence of antibody by commercial human ELISA. Then, ELISA was developed on microplates coated with hydatid fluid, Somatic and Excretory/secretory antigens of protoscolex of sheep. Results: The results of this study as analyzed by Kappa test showed that, hydatid fluid antigen could be used as a precise source of detection in indirect ELISA test. Conclusions: Hydatid fluid in comparison with Excretory-secretory and somatic antigens showed more compatibility agreement in kappa test which can be used for further studies in development of any ELISA test for diagnosis of human hydatidosis.

  4. An analytical investigation of the bullwhip effect in the food supply chain Un análisis investigativo del efecto chicote en la cadena de suministros de la industria alimenticia Uma análise investigativa do efeito chicote na cadeia de suprimentos da indústria alimentícia

    Directory of Open Access Journals (Sweden)

    Roberto Giro Moori

    2011-09-01

    Full Text Available This study, exploratory and descriptive in nature, aims to identify the relevant variables related to the bullwhip effect in the supply chain of the food industry. In the exploratory phase, to collect data in-depth interviews were made with owners and directors of eight supermarket companies in order to learn more about managing the food supply chain. In the descriptive phase, through semi-structured questionnaires, data were collected from a sample of 136 supermarket consumers, 13 wholesalers and 10 manufacturers. Data processed by content analysis (Bardin, 1977, descriptive statistics and nonparametric Kruskal-Wallis test, showed: a price as the main exogenous variable and cause of the bullwhip effect, b continuous replacement of products as an endogenous variable and amplifier of the bullwhip effect. With these results, it can be concluded that there is no evidence that the bullwhip effect is a variable managed by members of the supply chain in the food industry. Finally, this study contributes by showing empirically, an alternative to the usual theoretical mathematical models and simulations for the analysis of the bullwhip effect.Este trabajo, de naturaleza exploratoria y descriptiva, tiene por objetivo identificar las variables relevantes del efecto chicote en la cadena de suministros de la industria alimenticia. En la fase exploratoria, para la colecta de datos, el autor realizó entrevistas en profundidad a los propietarios y directores de ocho empresas supermercadistas, con la finalidad de conocer un poco más sobre la gerencia de la cadena de suministros de alimentos. En la fase descriptiva, mediante cuestionarios semiestructurados, fueron colectados datos de una muestra de 136 consumidores de supermercados, 13 mayoristas y 10 fabricantes. Los datos tratados por el análisis del contenido (BARDIN, 1977, estadísticas descriptivas y teste no paramétrico de Kruskal-Wallis, revelaron: a el precio como la principal variable exógena y

  5. ELISA study of oocyst-sporozoite transition in malaria vectors

    Directory of Open Access Journals (Sweden)

    Czeher C.

    2006-09-01

    Full Text Available Intrinsic vector characteristics and environmental factors affect the sporogonic development of P. falciparum in Anopheles mosquitoes. We tested for the presence of the circumsporozoite protein, as a marker of the oocyst to sporozoite transition in naturally infected Anopheles gambiae s.l. and Anopheles funestus. Malaria vectors were collected in a village in the Sahel of Niger during the rainy and dry seasons. ELISA-CSP was carried out on abdomen and head/thorax portions from more than 2,000 samples. No significant difference was found in the overall rates of infection of An. gambiae s.l. (4.13% and An. funestus (3.58%. Given the differences in duration of the two parasite stages, P. falciparum CSP antigen prevalence was nearly as high in the abdomen as in the head/thorax, and did not differ significantly between An. gambiae s.l. and An. funestus. These preliminary results suggest that development from oocysts to salivary gland sporozoites is similar in the two vectors. However, these developmental indices varied as a function of the season in which samples were collected, particularly for An. gambiae s.l. This simple method may be useful for field studies assessing the effect of environmental and genetic factors on parasite survival.

  6. Paper-based ELISA to rapidly detect Escherichia coli.

    Science.gov (United States)

    Shih, Cheng-Min; Chang, Chia-Ling; Hsu, Min-Yen; Lin, Jyun-Yu; Kuan, Chen-Meng; Wang, Hsi-Kai; Huang, Chun-Te; Chung, Mu-Chi; Huang, Kui-Chou; Hsu, Cheng-En; Wang, Chun-Yuan; Shen, Ying-Cheng; Cheng, Chao-Min

    2015-12-01

    Escherichia coli is a generic indicator of fecal contamination, and certain serotypes cause food- and water-borne illness such as O157:H7. In the clinic, detection of bacteriuria, which is often due to E. coli, is critical before certain surgical procedures or in cases of nosocomial infection to prevent further adverse events such as postoperative infection or sepsis. In low- and middle-income countries, where insufficient equipment and facilities preclude modern methods of detection, a simple, low-cost diagnostic device to detect E. coli in water and in the clinic will have significant impact. We have developed a simple paper-based colorimetric platform to detect E. coli contamination in 5h. On this platform, the mean color intensity for samples with 10(5)cells/mL is 0.118±0.002 (n=4), and 0.0145±0.003 (Ppaper-based ELISA is an innovative point-of-care diagnostic tool to rapidly detect E. coli, and possibly other pathogens when customized as appropriate, especially in areas that lack advanced clinical equipment.

  7. Estimation of genetic parameters and transmitting ability for Minnesota Johne’s milk ELISA test

    Science.gov (United States)

    A total number of 45,907 results for Johne’s Milk ELISA test were received from Minnesota DHIA, of which 40,177 records were from herds with at least one positive Johne’s cow. Pedigree information was available for 19,304 Holstein cows from AIPL USDA representing 22,694 total records. Milk ELISA opt...

  8. EMT nurjas Rate.ee ostuga Elisa kõnekaardiplaani / Gert D. Hankewitz

    Index Scriptorium Estoniae

    Hankewitz, Gert D.

    2006-01-01

    Ilmunud ka: Delovõje Vedomosti 12. apr. lk. 2. Elisa juhatuse esimehe Sami Seppäneni sõnul ostis EMT suhtlusportaali Rate.ee, kuna sai teada, et Elisa ning portaal plaanivad koos uue kõnekaardi turule tuua

  9. Reactivity in ELISA with DNA-loaded nucleosomes in patients with proliferative lupus nephritis

    NARCIS (Netherlands)

    Dieker, J.W.; Schlumberger, W.; McHugh, N.; Hamann, P.; Vlag, J. van der; Berden, J.H.M.

    2015-01-01

    Autoantibodies against nucleosomes are considered a hallmark of systemic lupus erythematosus (SLE). We compared in patients with proliferative lupus nephritis the diagnostic usefulness of a dsDNA-loaded nucleosome ELISA (anti-dsDNA-NcX) with ELISAs in which dsDNA or nucleosomes alone were coated. Fi

  10. Elisa ja EMT kemplevad, kumb pakub paremat levi / Toivo Tänavsuu

    Index Scriptorium Estoniae

    Tänavsuu, Toivo

    2007-01-01

    Mobiilifirma Elisa väitel on Tartus kõige kvaliteetsem leviala Elisal. EMT võrgujuhi Margus Malvi hinnangul on EMT ja Elisa võrgud Tartus samaväärsed, ainus erinevus on kliendi kaugus tugijaamast. Lisa: Mobiililevi kvaliteet

  11. Development of a Novel PmpD-N ELISA for Chlamydia psittaci Infection

    Institute of Scientific and Technical Information of China (English)

    LIU Shan Shan; CHU Jun; ZHANG Qiang; SUN Wei; ZHANG Tian Yuan; HE Cheng

    2016-01-01

    ObjectiveChlamydia psittaci is an avian respiratory pathogen and zoonotic agent.The wide prevalence ofC. psittaci poses a threat to the poultry industry and its employees. However, few commercial kits are available for detecting avian antibodies excluding the in-house ELISA kit. In this study, we developed a novel ELISA kit for detecting antibodies againstC. psittaci based on the N-terminal fragment of polymorphic outer membrane protein D (PmpD-N) as the coating antigen. Methods The antigen concentrations, primary antibody, and cut-off value were determined and optimized. The ELISA, designated PmpD-N ELISA, was assessed for sensitivity, specificity, and concordance using sera samples from 48 experimentally infected and 168 uninfected SPF chickens. Results The sensitivity and specificity of PmpD-N ELISA were 97.9%, 100%, respectively, while the concordance was 98.1% as compared to that of MOMP-ELISA. No cross-reaction with positive sera for other avian pathogens was found. Using PmpD-N ELISA, 799/836 clinical samples were positive, including 93.0% and 98.1% positivity in layers and broilers, respectively. Conclusion These data indicate that indirect ELISA with PmpD-N as the antigen candidate is a promising approach for the surveillance ofC. psittaci infection.

  12. Muestras y representatividad en vigilancia epidemiologica mediante sitios centinelas

    Directory of Open Access Journals (Sweden)

    Juan Samaja

    1996-09-01

    Full Text Available El artículo sostiene que las exigencias técnicas del muestreo para la vigilancia epidemiológica, exigen una revisión profunda de importantes conceptos de la Teoría de la Salud. En particular, es necesario hacer énfasis en las condiciones de vida, y, más específicamente, en los ambientes o contextos en que se desarrollan los procesos reproductivos de la vida social. Pero ambos campos temáticos exigen potenciar el acceso a datos más ricos que los que aportan las fuentes tradicionales. Este enfoque de la "vigilancia epidemiológica" exige una revisión de los tipos de muestras, y esto implica revisar las interpretaciones dominantes sobre los fundamentos lógicos de las inferencias a partir de muestras. Se torna necesario dejar atrás las muestras estadísticas (aún las estratificadas y promover procedimientos del tipo de los "sitios centinelas". Esta técnica, aplicada originariamente en sociedades con sistemas estadísticos deficitarios, puede desarrollarse para constituirse en un complemento substancial del monitoreo de condiciones de vida incluso en sociedades con buenos sistemas de información. El artículo propone transformar el concepto de "sitio centinela" incorporandole el requisito de la "representatividad cualitativa" mediante muestreos finalísticos sustentados en tipologías previas de las unidades espacio-poblacionales.

  13. CAPTURA DE CO2 MEDIANTE TRANSPORTADORES SÓLIDOS

    Directory of Open Access Journals (Sweden)

    Carmen Forero

    2011-01-01

    Full Text Available La evaluación de transportadores de oxígeno (TO, basados en CuO y NiO sobre Al2O3 y preparados por impregnación, se llevó a cabo en una planta piloto de dos lechos fluidizados interconectados de 500 Wte, donde se utilizaron tanto metano como gas de síntesis como gas combustible. Además, se estudió el efecto de diferentes impurezas presentes en el gas combustible como azufre o hidrocarburos ligeros en la eficacia de combustión del proceso y en el comportamiento de los TO. Los resultados obtenidos mostraron que ambos TO son adecuados para la captura de CO2 mediante transportadores sólidos de oxígeno en el proceso de combustión de metano, gas de síntesis o metano con impurezas como hidrocarburos ligeros o azufre en el gas.

  14. Enrichment-ELISA for Detection of Salmonella typhi From Food and Water Samples

    Institute of Scientific and Technical Information of China (English)

    S.KUMAR; K.BALAKRISHNA; HV.BATRA

    2008-01-01

    Objective Development of monoclonal antibody based sandwich enzyme linked immunosorbant assay(sELISA)for rapid detection of Salmonella enterica serovar typhi(S.typhi) from food and water samples and optimization of enrichment procedures for use with the developed sELISA to increase the detection Sensitivity of the assay. Methods Spleen cells from BALB/c mice immunized with flagellin(H=d)antigen of S.typhi were fused with Sp2/0 myeloma cells.The hybridoma cell line specific to H=d antigen was established.characterized and ascites raised against one of these clones.The hypefimmune serum to flagellin antigen was raised in New Zealand White rabbits.An sELISA was developed using polyclonalantibody as capture and monoelonal antibody as detection antibody.To design the efficient culture sUrategies for use with the sELISA.different pre-enrichment and enrichment brothswere evaluated.The mediaincluded buffered peptonewater(BPW)and brain heart infusion broth for pre-enrichment and selenite F broth and Rappaport-Vassiliadis broth as enrichment broths.The developed sELISA with preceding enrichment step in BPW(Enrichment-ELISA)was evaluated in various food samples artificially inoculated with S. typhi bacteria.Various food(30)and water(35)samples collected from field were also tested by Enrichment-ELISA and culture method. Results Out of four specific clones to H=d antigen,one clone(#2/56.IgG2a isotype)was usedin sELISA.The sELISA had the detectionlimit of 104-105 cfu of S.typhi.Of the various broths used with sELISA,BPW was found to yield maximum ELISA values.Enrichment-ELISA,when tested in artificially inoculated food samples,generally,could detect 102S.typhi cfu/mL within 10 h from variousfood rinses(meat,vegetable)and milk samples.After overnight enrichment in BPW,as less as 2 bacteria per 10 mL of milk,meat rinse.and chicken rinse could be detected.Only one of the field samples(water)gave false positive result by Enrichment-ELISA.Conclusion In comparison to culture

  15. eLISA eccentricity measurements as tracers of binary black hole formation

    CERN Document Server

    Nishizawa, Atsushi; Klein, Antoine; Sesana, Alberto

    2016-01-01

    Up to hundreds of black hole binaries individually resolvable by eLISA will coalesce in the Advanced LIGO/Virgo band within ten years, allowing for multi-band gravitational wave observations. Binaries formed via dynamical interactions in dense star clusters are expected to have eccentricities $e_0\\sim 10^{-3}$-$10^{-1}$ at the frequencies $f_0=10^{-2}$ Hz where eLISA is most sensitive, while binaries formed in the field should have negligible eccentricity in both frequency bands. We estimate that eLISA should always be able to detect a nonzero $e_0$ whenever $e_0\\gtrsim 10^{-2}$; if $e_0\\sim 10^{-3}$, eLISA should detect nonzero eccentricity for a fraction $\\sim 90\\%$ ($\\sim 25\\%$) of binaries when the observation time is $T_{\\rm obs}=5$ ($2$) years, respectively. Therefore eLISA observations of BH binaries have the potential to distinguish between field and cluster formation scenarios.

  16. Enzyme linked immunosorbent assay (ELISA) used for the detection of Cysticercus bovis injection in cattle

    International Nuclear Information System (INIS)

    Sensitivity and specificity of the ELISA for the diagnosis of T. saginata cysticercosis were studied. The specificity of the ELISA, by using ThFAS-L antigen, was found to be quite high when the hyperimmune serum samples of animals contained relatively high numbers of metacestodes. There was a direct relationship between the intensity of infection and the ELISA values of hyperimmune serum samples. Although ThFAS-L did not cross-react with F. hepatica and E. granulosus and gave no false positive reactions, it is not possible to regard this test as sensitive enough to detect naturally and weakly infected animals. Sensitivity and specificity of the ELISA may be improved by elution of the protein of MW 21800, which showed a diagnostic significance during immunoblot analysis. At present, the ELISA using ThFAS-L antigen has limited use in the diagnosis of cysticercosis infections in cattle

  17. Sensibilidad del cultivo de pool fecal para detectar infección por Mycobacterium avium subsp. paratuberculosis en rebaños bovinos de leche y su relación con la prueba de ELISA Sensitivity of pooled faecal culture for detecting Mycobacterium avium subsp. paratuberculosis infection in dairy herds and its relationship with the ELISA test

    Directory of Open Access Journals (Sweden)

    M Pradenas

    2008-01-01

    Full Text Available El objetivo del presente estudio fue evaluar la sensibilidad y especificidad del cultivo de pool fecal como alternativa diagnóstica de paratuberculosis en rebaños lecheros bovinos comparado con el cultivo fecal individual y la prueba de ELISA con la finalidad de reducir costos asociados al diagnóstico de la enfermedad. En 12 rebaños lecheros con antecedentes de paratuberculosis se recolectó un máximo de 50 muestras fecales individuales por rebaño (n=598 y se cultivaron en medio de Herrold en forma individual y en pools estratégicos de 5 y 10 animales. Simultáneamente, de cada animal se recolectó una muestra de sangre para la prueba de ELISA. Se evaluó la sensibilidad y especificidad de las pruebas usando como referencia los cultivos fecales individuales mediante tablas de dos entradas. En 10 (83,3% rebaños y en 42 (7% animales fue posible aislar Map en tanto que el 15,8% y 22% de los pools de 5 y 10 animales, respectivamente, resultaron positivos al cultivo fecal. La sensibilidad de los pools fecales de 5 y 10 animales fue 43,2% y 46,4%, respectivamente, mientras que la sensibilidad del ELISA fue 42,9%. El costo de los pools fecales de 5 animales fue similar al de la prueba de ELISA, pero cuatro veces menor que el costo del cultivo fecal individual; sin embargo, la sensibilidad y especificidad de los pools de 5 animales fue similar a los cultivos individuales. El cultivo de pools fecales demostró ser una buena y costo-efectiva alternativa para el diagnóstico de paratuberculosis para detectar rebaños infectados con MapThe aim of the present study was to evaluate the sensitivity and specificity of pooled faecal culture as a better alternative for the diagnosis of paratuberculosis in dairy herds compared to the conventional faecal culture and the ELISA test. Individual faecal and blood samples were collected from 50 cows in each one of 12 dairy herds (n = 598 with a history of paratuberculosis. Faecal samples were cultured on Herrold

  18. Caracterización de variables cinemáticas de la marcha humana mediante un sistema computarizado de captura de imágenes subacuáticas: parámetros angulares de rodilla

    Directory of Open Access Journals (Sweden)

    Mauricio Hernando Valencia

    2014-01-01

    Full Text Available Antecedentes. El proceso de ejecución de las terapias en el agua, realizadas en varios centros de rehabilitación en el entorno nacional e internacional, requiere de un sistema de medición. Objetivo. Validar la posibilidad de cuantificar las variables cinemáticas de la marcha humana, en individuos sanos, usando video fotogrametría como sistema computarizado de captura de imágenes subacuáticas con registro en plano sagital. Materiales y métodos. Mediante un sistema robótico que consta de un sistema de cadena y una plataforma móvil en el piso del tanque de marcha, que ajusta la posición final de la cámara submarina, se realizó análisis de la marcha y posterior cálculo de los ángulos articulares en ambiente acuático de cuatro individuos voluntarios. Resultados. El ángulo filtrado de flexión de la rodilla durante la fase de balanceo en el ciclo de marcha subacuática es mayor en el género masculino (52,2±9,7 que en el femenino (45,6±7,0. El ángulo filtrado de flexión de la rodilla durante la fase de apoyo en el ciclo de marcha subacuática es mayor en el género masculino (1,6±1,5 que en el femenino (0,1±0,01. Conclusiones. Se pueden cuantificar las variables cinemáticas de la marcha humana (parámetros angulares de la rodilla, en individuos sanos, usando video fotogrametría como sistema computarizado de captura de imágenes subacuáticas, en el tanque de marcha.

  19. Comparative Study of Three Different ELISA to Measure the Antibodies Against Infectious Bronchitis Virus in Vaccinated and Unvaccinated Broilers

    OpenAIRE

    Cardoso TC; Oliveira. C.; SEL Da-Silva; HL Ferreira; Pinto AA

    2001-01-01

    Broilers were spray-vaccinated (n=150) with H120 serotype at one-day-old, challenged after 28 days with M41 IBV serotype and after bled at day 28, 34 and 46 after challenged. The respective sera were tested by the indirect ELISA (I-ELISA), sandwich ELISA (S-ELISA), liquid phase blocking ELISA (LPB-ELISA) and the standard serum neutralization test (SNT). For this purpose, a total of 300 sera samples, 150 from non vaccinated and 150 from vaccinated broilers were titrated by all the serological ...

  20. Modelado de sistemas complejos mediante simulación basada en agentes y mediante dinámica de sistemas

    Directory of Open Access Journals (Sweden)

    LUIS R. IZQUIERDO

    2008-01-01

    Full Text Available Este trabajo compara dos técnicas de modelado de sistemas complejos: la simulación basada en agentes y la dinámica de sistemas. Esta comparación se lleva a cabo dentro del marco general del proceso de modelado científico. Los autores concluyen que la principal diferencia entre las dos metodologías se encuentra en el proceso de abstracción que cada una de ellas realiza para construir el modelo formal a partir del sistema complejo observado. Esta diferencia inicial se extiende a las restantes etapas del proceso de modelado científico. Finalmente, se indican los principales factores y las propiedades generales de un sistema complejo que hacen que una u otra técnica sea más relevante, aunque los autores destacan que, en la mayoría de los casos, modelizar un mismo sistema mediante las dos técnicas es la solución idónea.

  1. The FAO/IAEA ELISA kit quality assurance programme

    International Nuclear Information System (INIS)

    A system for quality assurance was first introduced into the rinderpest sero-monitoring network in 1989 and was designed to ascertain how well the FAO/IAEA rinderpest ELISA kit was functioning in individual laboratories. Of the 15 laboratories actively using the kit at that time, 9 submitted results. These showed that the kit was working well although the overall binding ratio (an indication of the ability of the assay to separate positive from negative sera) was sub-optimal. The results also revealed that in every laboratory the local negative population gave a lower value than the kit reference negative sera. This was not unexpected, as the kit reference negative serum was never intended to be used for the calculation of negative/positive cut-off values and was included only as a reference. The examination of local negative sera and the establishment of the mean local population negative value is essential to the establishment of the test in any laboratory. An examination of individual laboratory results indicated that where problems were encountered it was invariably due to the use of poor water for reconstitution of reagents. This has confirmed the need to supply sterile de-ionized water for re-constitution of all kit reagents. The results from this quality assurance exercise proved vital in identifying problems with the kit itself and with the use of the kit in individual laboratories. In the most part these problems have now been resolved. However, in retrospect it is considered that the approach adopted was over-complicated and in future quality assurance will involve the testing of a panel of 44 sera distributed to all participating laboratories once a year. (author). 6 figs, 2 tabs

  2. La cadena alimentaria "carne de bovino" en México: factores a considerar en la integración de los eslabones criador y finalizador

    Directory of Open Access Journals (Sweden)

    J. M. Zorrilla

    2010-01-01

    Full Text Available Una de las debilidades de las cadenas alimentarias en México es su falta de integración adecuada que les permita posicionarse como la primera opción del consumidor final. La producción de carne de bovino no está exenta de este señalamiento, condición que ha motivado el diseño e implementación de diferentes programas oficiales encaminados a la integración de los eslabones primarios, criador y finalizador. Para que estos programas sean sustentables a mediano y largo plazo requieren enfrentar, en forma racional, el reto de incorporar -en su diseño- enfoques e intereses gremiales de índole económicos, que si bien son interdependientes, frecuentemente son, a la vez, dispares tanto entre los eslabones como dentro de ellos mismos. Consecuentemente, el abordaje de estas acciones se beneficiará de disponer de análisis y propuestas desde posiciones imparciales, independientes de los intereses particulares de los participantes incluyendo -entre éstos, además de los eslabones productivos primarios- al mismo poder ejecutivo, cabeza del sector, el cual, por razones de políticas gubernamentales a las que está sujeto, se ve inclinado a dar preferencia a acciones con tintes extra-curriculares. Por lo expuesto anteriormente, el sector académico de las universidades públicas, con base en su compromiso social, se involucra como un ente imparcial en estos procesos de construcción de acuerdos, aportando elementos de análisis y sugerencias para que los esfuerzos de integración sectorial rindan los resultados esperados a mediano y largo plazo, en beneficio de la sociedad. Con este marco referencial en mente se elaboró el presente documento.

  3. APLICACIÓN DE LA TOMA DE DECISIONES MULTICRITERIO EN LA CADENA DE CORTE, ALZA Y TIRO DE LA CAÑA DE AZÚCAR

    Directory of Open Access Journals (Sweden)

    Fernando Marrero Delgado

    2001-01-01

    Full Text Available

    La situación actual en que se encuentra la economía cubana hace necesario que se eleve su eficacia a fin de contribuir a su recuperación. Al ser esta una de las ramas principales, es imprescindible aplicar técnicas que permitan asegurar un funcionamiento óptimo de cada una de sus etapas y áreas, siendo la cadena de corte, alza y transportación de la caña de azúcar el eslabón que vincula a la agricultura con la industria y donde se incurre en una serie de costos que afectan decisivamente el costo total del proceso de obtención de azúcar; por lo que es importante evaluar la misma con varios criterios de desempeño. Por esta razón, es imprescindible el uso de la óptica multicriterio para la toma de decisiones en esta área y precisamente aquí radica el objetivo del presente trabajo, donde se presenta un modelo multimeta para evaluar el desenvolvimiento de la transportación de la caña desde el campo hasta la industria

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  4. A Novel Blocking ELISA for Detection of Antibodies against Hepatitis E Virus in Domestic Pigs.

    Science.gov (United States)

    Chen, Yiyang; Zhao, Qin; Liu, Baoyuan; Wang, Lizhen; Sun, Yani; Li, Huixia; Wang, Xinjie; Syed, Shahid Faraz; Zhang, Gaiping; Zhou, En-Min

    2016-01-01

    Hepatitis E virus (HEV) infects both humans and animals, with an overall human mortality rate generally less than 1%, but as high as 20% among pregnant women. HEV strains fall into 4 major genotypes. Zoonotic genotypes 3 and 4 associate with sporadic human and animal HEV cases in many industrialized countries. To date, collective evidence implicates pigs as the main HEV reservoir, justifying the importance of monitoring HEV infection rates in pig herds to prevent human illness. Due to the lack of a robust in vitro cell culture system for viral propagation, no "gold standard" assay has yet been developed to detect HEV infection in domestic pigs. 1E4, a monoclonal antibody (mAb) specific for the C-terminal 268 amino acids of HEV genotype 4 ORF2 capsid protein (sORF2-C), was generated and conjugated to horseradish peroxidase (HRP) for use in a blocking ELISA (bELISA). Optimal sORF2-C coating antigen concentration (8 μg/ml), HRP-1E4 dilution (1:1000), and test pig serum dilution (1:20) were determined using a checkerboard titration test. A cut-off value of 16.9% was chosen to differentiate between positive vs. negative sera after mean percent inhibition (PI) testing of 230 negative pig sera. Compared with the indirect ELISA (iELISA), western blot, and a commercial ELISA kit for detecting anti-HEV antibodies in human sera, the bELISA showed no statistical differences and statistically high coincidence of 93.23%, 92%, and 95% with the other tests, respectively. A blocking ELISA (bELISA) for detecting anti-HEV antibodies in pig serum samples was developed with high sensitivity and high specificity comparable to that of the indirect ELISA. The bELISA results exhibited high agreement with iELISA, western blot, and a commercial ELISA kit designed to detect human anti-HEV antibodies. Therefore, bELISA should serve as an ideal method for large-scale serological investigation of anti-HEV antibodies in domestic pigs.

  5. A Novel Blocking ELISA for Detection of Antibodies against Hepatitis E Virus in Domestic Pigs.

    Directory of Open Access Journals (Sweden)

    Yiyang Chen

    Full Text Available Hepatitis E virus (HEV infects both humans and animals, with an overall human mortality rate generally less than 1%, but as high as 20% among pregnant women. HEV strains fall into 4 major genotypes. Zoonotic genotypes 3 and 4 associate with sporadic human and animal HEV cases in many industrialized countries. To date, collective evidence implicates pigs as the main HEV reservoir, justifying the importance of monitoring HEV infection rates in pig herds to prevent human illness. Due to the lack of a robust in vitro cell culture system for viral propagation, no "gold standard" assay has yet been developed to detect HEV infection in domestic pigs. 1E4, a monoclonal antibody (mAb specific for the C-terminal 268 amino acids of HEV genotype 4 ORF2 capsid protein (sORF2-C, was generated and conjugated to horseradish peroxidase (HRP for use in a blocking ELISA (bELISA. Optimal sORF2-C coating antigen concentration (8 μg/ml, HRP-1E4 dilution (1:1000, and test pig serum dilution (1:20 were determined using a checkerboard titration test. A cut-off value of 16.9% was chosen to differentiate between positive vs. negative sera after mean percent inhibition (PI testing of 230 negative pig sera. Compared with the indirect ELISA (iELISA, western blot, and a commercial ELISA kit for detecting anti-HEV antibodies in human sera, the bELISA showed no statistical differences and statistically high coincidence of 93.23%, 92%, and 95% with the other tests, respectively. A blocking ELISA (bELISA for detecting anti-HEV antibodies in pig serum samples was developed with high sensitivity and high specificity comparable to that of the indirect ELISA. The bELISA results exhibited high agreement with iELISA, western blot, and a commercial ELISA kit designed to detect human anti-HEV antibodies. Therefore, bELISA should serve as an ideal method for large-scale serological investigation of anti-HEV antibodies in domestic pigs.

  6. Development and comparative evaluation of a competitive ELISA with rose bengal test and a commercial indirect ELISA for serological diagnosis of brucellosis.

    Science.gov (United States)

    Mythili, Tadepalli; Rajendra, Lingala; Bhavesh, Trangadia; Thiagarajan, Dorairajan; Srinivasan, Villuppanoor Alwar

    2011-10-01

    The development of a competitive ELISA for the detection of brucella-specific antibodies in bovines is described. Anti-brucella guinea pig serum was used as a source of competing antibodies. Lipo-polysaccharide purified from inactivated B. abortus S19 culture was used as antigen for the development of the assay. Sera from cattle were used in the competitive ELISA, rose bengal test and a commercial indirect ELISA. The following cattle sera were tested: (i) known positive sera (n = 80) (ii) known negative sera (n = 100) and (iii) field sera (n = 1184). Based on the receiver operating characteristics curve analysis and frequency distribution of the percentage of inhibition, 30% inhibition was considered the cut-off for positive and negative results. The sensitivity and specificity estimate on comparison with the commercial indirect ELISA was 94.87 and 92.12% respectively. The competitive ELISA described is a simple method for the routine screening of animal sera for detecting Brucella-specific antibodies. PMID:23024418

  7. La Gestión del Conocimiento en la Cadena de Suministro: Análisis de la Influencia del Contexto Organizativo Knowledge Management in the Supply Chain: Analysis of the Influence of the Organizational Context

    Directory of Open Access Journals (Sweden)

    Josep Capó-Vicedo

    2007-01-01

    Full Text Available El objetivo de este artículo es analizar que estructura o modelo organizativo es el más apropiado para que se dé una verdadera gestión del conocimiento en una cadena de suministro. Para ello se realiza una breve revisión de alguna de las aportaciones más significativas en la literatura existente sobre la relación entre la gestión del conocimiento y el contexto organizativo, así como sobre los requerimientos necesarios para gestionar el conocimiento en un contexto interorganizativo, como es el existente en una cadena de suministro. Del análisis realizado se concluye que la estructura organizativa conocida como Empresa Virtual es la más apropiada para gestionar el conocimiento en una cadena de suministro, por sus características intrínsecas de confianza mutua entre sus miembros, así como por el dinamismo en su creación y operaciónThe aim of this paper is to analyze which organizational structure or model is the most suitable so that a real knowledge management in a supply chain takes place. To achieve this objective, a brief revision of the existing literature related to the relationship between knowledge management and the organizational context and the necessary requirements to manage the knowledge in an inter-organizational context is done. From this analysis, it is concluded that the Virtual Enterprise is the most suitable organizational structure to manage knowledge in a supply chain, based on the intrinsic features of mutual confidence between its members and the dynamism in its creation and operation

  8. Detection of Mycoplasma Infection in Cell Cultures by PCR-ELISA

    Directory of Open Access Journals (Sweden)

    Mohammad Reza Arabestani

    2014-03-01

    Full Text Available Abstract Background and objective: Mycoplasmas, particularly species of the genera Mycoplasma and Acholeplasma, are known to be occasional microbial contaminants of cell cultures that produce biologics. The aim of this study is to alternate a specific, sensitive and rapid method for detection of a variety of Mycoplasma species in cell lines by PCR-ELISA. Material and methods: This method was based on a PCR-ELISA reaction using genus, specific primer and species specific primers for Mycoplasma species that labeled with Digoxigenine and specific probe, which labeled by biotin. Results: Mycoplasma contamination using PCR-ELISA was examined for 183 different cell line deposited in national cell bank of Iran. PCR-ELISA showed that 48.6 % of cell lines were contaminated whit Mycoplasma while 27.3% of them were found to be infected with microbial culture. PCR and PCR-ELISA methods, both of them showed the same results. In comparison to microbiological culture, PCR-ELISA method was shown to be 100% sensitive and 70.7% specific. Conclusions: Microbial culture and staining are common methods for detection of cell lines Mycoplasma contamination. These methods are time-consuming and so rapid contamination detection is critical for cell lines. For this reason PCR-ELISA method is recommended for rapid mycoplasma detection.

  9. Integración de los sistemas de información para la gestión de la cadena de suministro : un estudio empírico en grandes empresas

    OpenAIRE

    Gonzálvez Gallego, Nicolás

    2016-01-01

    Objetivos El objetivo general de esta investigación es la generación de conocimiento en torno a la relación entre las TIC, considerando de manera particular los sistemas de información, y la generación de resultados en el marco de la gestión de la cadena de suministro de grandes empresas. De manera más específica, las publicaciones de esta Tesis Doctoral persiguen dar respuesta a las siguientes preguntas de investigación: - ¿Existe una relación directa entre el uso de las TIC en proces...

  10. Desarrollo de métodos de detección de salmonella basados en la reacción en cadena de la polimerasa y su validación en muestras alimentarias

    OpenAIRE

    Martínez Ballesteros, Ilargi

    2014-01-01

    224 p. [ES] Salmonella es uno de los patógenos alimentarios más importantes a nivel mundial y causante de enfermedades gastroentéricas en humanos y animales. La presencia de Salmonella en un alimento, sea en la cantidad que sea, se considera peligroso y por lo tanto no será apto para el consumo humano. Por ello, los controles microbiológicos rutinarios en la cadena alimentaria son de vital importancia para no comprometer la salud del consumidor. Los controles se deben rea...

  11. Detección del ADN de Papilomavirus humano en lesiones precancerosas y cancerosas de cuello uterino por reacción en cadena de la polimerasa, en Bucaramanga, Santander

    Directory of Open Access Journals (Sweden)

    Castillo A.

    2001-06-01

    Full Text Available Determinar la validez de la Reacción en Cadena de la Polimerasa (PCR en el diagnóstico de la infección por el virus del papiloma humano (VPH, detectando diferentes tipos de VPH oncogénicos (16, 18, 31, 33 y 35 y no oncogénicos (6 y 11 en pacientes con lesiones precancerosas y cancerosas de cérvix y en pacientes control, estableciendo la prevalencia del virus en estas lesiones.

  12. La cadena tecnológica operativa como una herramienta teórica y metodológica. Una perspectiva desde los planteamientos de la arqueología del paisaje

    Directory of Open Access Journals (Sweden)

    Cobas-Fernández, Isabel

    2001-12-01

    Full Text Available We will focus on two main topics: first, the special approach to the Technological Chain (TC that we have used to study NW Iberian protohistoric and prehistoric pottery from a landscape archaeology viewpoint; and second, the specific problems that we found while trying to perform such work. Regarding the first point, we believe that the most innovative issue is that the TC is not only seen as a mere descriptive tool for technological facts, but we have tried to relate such facts with the social context in which they occur. As a result of it, we have divided the TC into three deeply intertwined aspects: the technical aspects or technological chain, the social aspects or conceptual chain, and the final result of those processes, úiQ final product, which comprises the last link in the chain. Concerning the second point, we have found important obstacles in trying to get to the technological aspects, because the necessary auxiliary techniques are not always readily available. Also, gaining knowledge of the imaginary dimension is even more difficult, as we approach it solely from its material demonstration.

    Centraremos nuestra comunicación en dos aspectos: el enfoque particular que hemos dado a la Cadena Técnica Operativa en el marco de la Arqueología del Paisaje para aplicarlo al estudio de la cerámica prehistórica y protohistórica del NW de la Península Ibérica y los problemas con los que nos hemos encontrado para la aplicación práctica de esta herramienta en dicho contexto. Por 10 que respecta al primer punto, creemos que la mayor novedad reside en que la CTO no se considera como un útil meramente descriptivo de hechos tecnológicos sino que se intenta relacionar estos hechos con el contexto social en el que se producen. Por esta razón hemos dividido la CTO en tres aspectos profundamente imbricados: los aspectos técnicos, considerados en la cadena técnica, los aspectos sociales, comprendidos en la cadena conceptual y

  13. El síndrome de fatiga crónica: del modelo de susceptibilidad a la hipótesis de la cadena de estrés en el área laboral

    OpenAIRE

    Fernando Mansilla Izquierdo

    2003-01-01

    El modelo de susceptibilidad tiene en cuenta la combinación de algunos elementos: la susceptibilidad biológica, la susceptibilidad al mobbing, la susceptibilidad al burn-out, la susceptibilidad al estrés laboral, y los factores de protección como: apoyo social, autoconcepto positivo, locus de control interno, alta autoestima y sentimiento de autoeficacia. La hipótesis de la cadena de estrés en el área laboral considera que la persistencia del estrés laboral es acumulativa. El mobbing, el sínd...

  14. "Tintorerías de posguerra" e innovación organizativa en Inditex: una perspectiva contractual de la gestión lean de la cadena de suministro

    OpenAIRE

    Ana I. Martínez-Senra; Antonio Sartal; Xosé H. Vázquez

    2012-01-01

    La evolución reciente del mercado, con cambios de preferencias más frecuentes y exigencias de personalización crecientes, ha puesto de manifiesto la fragilidad de aquellas empresas cuya externalización offshore no ha considerado los costes de coordinar proveedores con localizaciones lejanas. El artículo analiza este problema utilizando el caso de Inditex; una empresa que, inspirándose en las tintorerías españolas de los años cuarenta, ha reformulado la gestión de su cadena de suministro dando...

  15. Diseño de la cadena productiva sostenible del espárrago verde fresco, como alternativa de diversificación de la zona cafetera de las subregiones centro sur y bajo occidente del Departamento de Caldas

    OpenAIRE

    Salazar Bernal, Ramiro; Betancourt Escobar, Ángela María; Betancurt Quintero, Vivian Marcela; Cuellar Pavas, Oscar H.

    2004-01-01

    El diseño de la cadena productiva sostenible del espárrago verde fresco como alternativa de diversificación para la zona cafetera de las subregiones centro sur y bajo occidente del departamento de Caldas, como estudio y propuesta desarrollada por: Ramiro Salazar B. Ingeniero Industrial, Ángela María Betancourt E. Diseñadora Industrial, Oscar Cuella, Contador y Vivian Marcela Betancurt Q. Administradora de Empresas con énfasis en economía solidaria; se fundamenta y articula al plan de desarrol...

  16. Graphical control and evaluation of the operational performance of ELISA method for detection of trypanosomal antibodies

    International Nuclear Information System (INIS)

    The performance of four indirect trypanosomal antibody detection enzyme-linked immunosorbent assays (I-TAB ELISA's), exploiting native and denatured antigens of Trypanosoma congolense (T.c.AGn, T.c.AGd) and T. vivax (T.v.AGn, T.v.AGd), has been evaluated in fifteen laboratories from Africa and Europe. Standardised internal quality control samples (IQCs) were used as indicators and data plotted on charts to monitor and control the ELISA's at individual laboratories. Based on the overall data, dispersion of true values from the population data range was estimated plotting the location and deviation of raw and normalised absorbance values of the IQCs. Binding ratios were calculated to estimate the assay proficiency with respect to the accuracy of assessing that the QC samples tested positive or negative in the test proper. The frequency distribution of coefficients of variation <10% of IQCs was monitored. The use of this standardised and transparent IQC data charting provides a useful quality control tool for the evaluation of the performance of the ELISA's used for trypanosomosis serology. The method provides a measure of confidence in estimating the proficiency of national laboratories with respect to reported ELISA results on disease occurrence. Moreover, data were compiled of the inter-laboratory ELISA performance by means of summary data charts with reference to the performance criteria described. The data were also analysed using modified Youden plots. The analysis demonstrated similar laboratory proficiency for the I-TAB ELISA (T.c.AGn) (four of five laboratories), I-TAB ELISA (T.c.AGd) (eleven of fourteen laboratories), I-TAB ELISA (T.v.AGn) (three of five laboratories), and I-TAB ELISA (T.v.AGd) (eleven of fifteen laboratories). The impact of data variation between test and specified values as a sole decision criterion for acceptance or rejection of test plates is discussed. (author)

  17. Quantification of Hordeins by ELISA: the correct standard makes a magnitude of difference.

    Directory of Open Access Journals (Sweden)

    Gregory J Tanner

    Full Text Available BACKGROUND: Coeliacs require a life-long gluten-free diet supported by accurate measurement of gluten (hordein in gluten-free food. The gluten-free food industry, with a value in excess of $6 billion in 2011, currently depends on two ELISA protocols calibrated against standards that may not be representative of the sample being assayed. AIM: The factors affecting the accuracy of ELISA analysis of hordeins in beer were examined. RESULTS: A simple alcohol-dithiothreitol extraction protocol successfully extracts the majority of hordeins from barley flour and malt. Primary hordein standards were purified by FPLC. ELISA detected different classes of purified hordeins with vastly different sensitivity. The dissociation constant (Kd for a given ELISA reaction with different hordeins varied by three orders of magnitude. The Kd of the same hordein determined by ELISA using different antibodies varied by up to two orders of magnitude. The choice of either ELISA kit or hordein standard may bias the results and confound interpretation. CONCLUSIONS: Accurate determination of hordein requires that the hordein standard used to calibrate the ELISA reaction be identical in composition to the hordeins present in the test substance. In practice it is not feasible to isolate a representative hordein standard from each test food. We suggest that mass spectrometry is more reliable than ELISA, as ELISA enumerates only the concentration of particular amino-acid epitopes which may vary between different hordeins and may not be related to the absolute hordein concentration. MS quantification is undertaken using peptides that are specific and unique enabling the quantification of individual hordein isoforms.

  18. Validación de un ELISA para la cuantificación de antitoxina tetánica en suero humano

    Directory of Open Access Journals (Sweden)

    Rolando Ochoa

    2000-12-01

    Full Text Available Se desarrolló un ensayo inmunoenzimático en fase sólida (ELISA de tipo indirecto para la cuantificación de antitoxina tetánica en suero humano, con el empleo de un estándar previamente calibrado. Las imprecisiones intra e interensayo fueron de alrededor del 10%. Este ensayo mostró una excelente exactitud, con valores de recuperación entre 90% y 110%. Las desviaciones del paralelismo estuvieron por debajo del 10%; éstas se evaluaron mediante diluciones que involucraban el rango de trabajo de la curva estándar. El límite de detección fue 0,0016 Unidades Internacionales por mL (UI/mL. El ensayo mostró una buena correlación con la prueba de neutralización (R2=0,9862. La ecuación de ajuste lineal fue: ELISA = 0,993 ensayo de neutralización – 0,2143. Se cuantificó la antitoxina tetánica en muestras de suero de 408 niños entre 1 a 5 años de edad,aleatoriamente seleccionados en Ciudad de La Habana y cada edad se analizó por separado. La mayor respuesta se alcanzó a los 2 años de edad, al completarse el esquema básico de inmunización. Todos los niños presentaron valores superiores al nivel mínimo protector (0,01 UI/mL.

  19. Fluorescence polarization assay, competitive enzyme-linked immunosorbent assay (ELISA-C and indirect ELISA for the diagnosis of brucellosis in buffaloes (Bubalus bubalis Teste de polarização fluorescente, teste imunoenzimático competitivo (ELISA-C e ELISA indireto para o sorodiagnóstico da brucelose em búfalos (Bubalus bubalis

    Directory of Open Access Journals (Sweden)

    Lília Márcia Silva Paulin

    2012-09-01

    Full Text Available The objective of the present study was to compare the performance of three serological tests for diagnosis of Brucella abortus infections in buffaloes (Bubalus bubalis. Serum samples collected from 696 adult females were submitted to the competitive enzyme-linked immunosorbent assay (ELISA-C, (I-ELISA, fluorescence polarization test (FPA, 2-mercaptoethanol test (2-ME and complement fixation test (CFT. The gold standard was the combination of CFT and 2-ME, considering as positive the reactors in both CFT and 2-ME, and as negative those non-reactors. ROC analyses were done for C-ELISA, I-ELISA and FPA and the Kappa agreement index were also calculated. The best combinations of relative sensitivity (SEr and relative specificity (SPr and Kappa were given by C-ELISA (96.9%, 99.1%, and 0.932, respectively and FPA (92.2%, 97.6 and 0.836, respectively. The C-ELISA and FPA were the most promising confirmatory tests for the serological diagnosis of brucellosis in buffaloes, and for these tests, cut-off values for buffaloes may be the same as those used for bovines.O presente estudo objetivou comparar o desempenho de três testes para o sorodiagnóstico da Brucella abortus em búfalos (Bubalus bubalis. Soros de 696 fêmeas bubalinas adultas foram submetidos aos testes: teste imunoenzimático indireto (ELISA-I, teste imunoenzimático competitivo (ELISA-C, teste de polarização fluorescente (TPF, 2-mercaptoetanol (2-ME e teste de fixação do complemento (FC. Foi empregada, como gold standard, a combinação de dois testes, FC e 2-ME. A curva ROC foi construída para os três testes: ELISA-I, ELISA-C e PF e, com base nos resultados desta análise, foi calculado o índice de concordância Kappa para cada teste. As melhores combinações de sensibilidade (Sr e especificidade (Er e os melhores resultados de Kappa foram alcançados pelo ELISA-C (96,9%, 99,1% e 0,932, respectivamente, seguido pelo PF (92,2%, 97,6%, e 0,836, respectivamente. Concluiu-se que os

  20. Performance indexes of a dot-enzime-linked immunosorbent assay (dot-ELISA and an ezyme-linked immunosorbent assay (IgG-ELISA for field surveys of new world leishmaniasis Índices de desempenho do dot-ELISA e do IgG-ELISA, em inquéritos soroepidemiológicos da leishmaniose do Novo Mundo

    Directory of Open Access Journals (Sweden)

    M. Carolina S. Guimarães

    1991-10-01

    Full Text Available Diagnostic performance indexes of sensitivity, specificity, positive predictive value and efficiency were determined for dot-ELISA and IgG-ELISA tests in 340 leishmaniasis sera. Sensitivity of the dot-ELISA was significantly lower than IgG-ELISA's; the two tests had indexes of specificity and positive predictive value of the same magnitude. Seventy-eight sera gave a negative dot-ELISA test result and a positive IgG-ELISA test result. When sera were classified according to different criteria as how to interpret this diversity, the kappa statistic did not corroborate the classification indicating that the two tests display a substantial strength of agreement. The results presented indicate that performance indexes accrued in a survey where variables arc well known may be extrapolated to other population studies if the disease presents itself as highly prevalent (due to a selection bias or not and may be expected to discriminate a disease status among test positives.Índices de desempenho diagnóstico de sensibilidade, especificidade, valor preditivo positivo e eficiência foram determinados nas reações de dot-ELISA e IgG-ELISA em 340 soros de leishmaniose mucocutânea. A sensibilidade do dot-ELISA foi significativamente mais baixa que a da IgG-ELISA e os dois testes tiveram índices de especificidade e de valor de predição positivo da mesma magnitude. Setenta e oito soros tinham resultado negativo à reação de dot-ELISA e resultado positivo ao IgG-ELISA. Quando os soros foram examinados de acordo com diferentes critérios de análise, quanto a esta discrepância a estatística kappa não corroborou esta hipótese indicando que os dois testes concordavam substancialmente. Os resultados apresentados indicam que os índices de desempenho diagnóstico obtidos em trabalhos de campo onde haja boa caracterização das variáveis podem ser extrapolados a outros estudos populacionais desde que a doença se mostre de alta prevalência (devido a um vi

  1. The enzyme immunosorbent assay (ELISA) for serologic diagnosis of toxoplasmosis in pregnancy.

    Science.gov (United States)

    Luerti, M; Corticelli, C; Santini, A; Privitera, G; Nardi, G

    1984-01-01

    The enzyme-linked immunosorbent assay (ELISA) is simple, quick, and inexpensive, characteristics that make it particularly suitable for large screening programs. We compared the results obtained by ELISA with those obtained by immunofluorescent antibody test (IFAT), indirect hemoagglutination test (IHAT), and Sabin Feldman Dye test in a group of 105 pregnant women in the course of a screening for toxoplasmosis. Prevalence of IgG antibody titers were not significantly different. ELISA results were particularly close to those of the reference tests (IFAT and Dye test).

  2. Detection of Ehrlichia canis in Dogs by IFA Test and Dot-ELISA

    OpenAIRE

    ERDEĞER, Jale

    2003-01-01

    Ehrlichia canis is the causative agent of canine monocytic ehrlichiosis (CME). The aim of this study was to detect E. canis antibodies in dogs by the IFA and dot-ELISA techniques. Blood sera from 239 dogs were collected as material. IFA test and dot-ELISA were performed with commercially available test kits. The IFA test was applied according to the procedure in the IFA kit. The Immunocomb test system was used in dot-ELISA. Agreement between these techniques was analysed by the kappa (K) tes...

  3. Design of absorbency photometer used in a fully automatic ELISA analyzer

    Science.gov (United States)

    Dong, Ningning; Zhu, Lianqing; Dong, Mingli; Niu, Shouwei

    2008-03-01

    Absorbency measurement is the most important step in the ELISA analysis. Based on the spectrophotometry, absorbency photometer system used in a fully automatic ELISA analyzer is developed. The system is one core module of the fully automatic ELISA analyzer. The principle and function of the system is analyzed. Three main units of the system, the photoelectric transform unit, the data processing unit and the communication and control unit, are designed and debugged. Finally, the test of the system is carried out using the verification plate. The experiment results agree well with the requirements.

  4. de teorías mediante un estudio de caso

    Directory of Open Access Journals (Sweden)

    Verónica Alonso Jiménez

    2008-01-01

    Full Text Available La democracia representativa como forma de gobierno, implica que el poder se ejerce por personas, que elegidas por el pueblo, actúan en su nombre y representación. El modo de participación en la elección y la manera como éstas se convierten en cargos públicos, requiere del dise- ño de instituciones que sistematicen dicha participación. La república de tipo presidencial, es una modalidad del gobierno electivo y popular, cuyo titular es el jefe del ejecutivo, electo por el pueblo o sus representantes, en donde el ejercicio del poder es limitado y mantiene un régimen de responsabilidades políticas. El diseño institucional que le corresponde a esta forma de gobierno es la parcelación del poder pú- blico en tres: poder ejecutivo, poder legislativo y poder judicial. Las ventajas políticas de este esquema es que la división de poderes, neutraliza el riesgo de caer en el autoritarismo, al impedir que el poder se concentre. La división de poderes es un dispositivo de restricción de facultades de los órganos estatales, por lo que no existe superioridad jerárquica entre los poderes, al contrario, cada órgano tiene bien delimitadas sus funciones y atribuciones, las que están reguladas por un marco jurídico común llamado Constitución. Y la división de poderes contribuye a mantener el equilibrio entre estos, mediante el llamado sistema de “pesos y contrapesos”. El presente trabajo se centra en el estudio de la Cámara de Diputados, considerada como una de las parcelas en las que está divida la autoridad del Estado, cuyo objetivo es la validación de los modelos teóricos en la tipificación del Congreso y la comprensión de las variables internas y externas que influyen en el comportamiento legislativo.

  5. Desarrollo de la lectura mediante estratégias integradoras

    Directory of Open Access Journals (Sweden)

    Solé, Maira

    2005-06-01

    Full Text Available La lectura y la escritura son procesos que cada día ameritan nuevos cambios y transformaciones. La propuesta de un Proyecto Pedagógico Integrador, (Fraca 2003 desarrollado con éxito en algunas instituciones venezolanas, se perfila como una alternativa significativa para el desarrollo de estos elementos. La idea o núcleo central es la integración de las diferentes asignaturas curriculares y lograr una globalización partiendo de sus objetivos y contenidos programáticos. El eje pedagógico integrador le permite al docente, evidenciar con mayor prontitud los resultados mediante actividades prácticas de lectura y escritura. Así mismo combina elementos claves del aprendizaje ausbeliano: información previa, información nueva y construcción de la información definitiva o integrada. La puesta en ejecución de las estrategias integradoras, en esta ocasión por maestros en formación (UNEG, a diferentes niños de escuelas del Estado Bolívar (Venezuela, certificando cómo la lectura y la escritura pueden tener un espacio ideal y significativo en la instrucción actual. Solo se necesita la intención, creatividad, dinamismo e ingenio. The reading and the writing plows processes that every day they require new changes and transformations. The proposal of an Integrative Pedagogic Project, (Fraca 2003 developed with success in some Venezuelan institutions; it is profiled like a significant alternative for the development of these elements. The idea or central nucleus is the integration of the different curricular subjects and to achieve a globalization leaving of its objectives and programmatic contents. The integrative pedagogic axis allows to the educational one, to evidence with more readiness the results by means of practical activities of reading and it notarizes. Likewise it combines key elements of the learning ausbeliano: previous information, new information and construction of the definitive or integrated information. The operation of

  6. Planificación táctica de las operaciones en cadenas de suministro de respuesta rápida (responsive) con estructura alternativa de procesos: modelado matemático, implementación y experimentación

    OpenAIRE

    CALDERÓN LAMA, JOSÉ LUIS

    2011-01-01

    Se define la Cadena de Suministro de Respuesta Rápida (Responsive Supply Chain) como aquella apropiada para productos innovadores con procesos estables. Esta Cadena de Suministro (CS) tiene que enfrentar una incertidumbre alta en la previsión de la demanda de sus múltiples productos (demanda que es además estacional y volátil). Interesa reducir los costes, las roturas de stock y el exceso de productos que deberán rematarse al final de cada temporada de ventas. El objetivo de esta tesis e...

  7. The time course of the specific antibody response by various ELISAs in pigs experimentally infected with Toxoplasma gondii

    DEFF Research Database (Denmark)

    Lind, Peter; Haugegaard, J.; Wingstrand, Anne;

    1997-01-01

    With the aim of developing routine serological tests for monitoring the Toxoplasma infection status of Danish swine herds, four ELISAs based on tachyzoite antigen were set up: (1) an indirect ELISA for IgG-antibody; (2) a blocking ELISA for antibody to the membrane antigen, P-30; (3) an indirect...

  8. The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA

    Directory of Open Access Journals (Sweden)

    G.H.T.S. Barbosa

    2000-07-01

    Full Text Available F1-antigen purified from Yersinia pestis was covalently linked to 5-mm diameter filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde. These discs were used both for ELISA and dot-ELISA for the detection of anti-F1 IgG in rabbits. The best conditions were achieved using 1.25 µg of F1 antigen/disc, 3% w/v skim milk in PBS as blocking agent, anti-IgG peroxidase conjugate diluted 12,000 times, and serum from rabbits immunized or not against Y. pestis, diluted 6,400 times. The absorbance values obtained from the comparative study between this procedure and conventional ELISA were not significantly different but the low cost of the reagents employed in ELISA using the filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde makes this method economically attractive.

  9. Prospects of eLISA for detecting Galactic binary black holes similar to GW150914

    Science.gov (United States)

    Seto, Naoki

    2016-07-01

    We discuss the prospects of eLISA for detecting gravitational waves (GWs) from Galactic binary black holes (BBHs) similar to GW150914. For a comoving merger rate that is consistent with current observation, eLISA is likely to identify at least one BBH with a sufficient signal-to-noise ratio. In addition, eLISA has a potential to measure the eccentricity of the BBH as small as e ˜ 0.02, corresponding to the residual value e ˜ 10-6 at 10 Hz. Therefore, eLISA could provide us with a crucial information to understand the formation processes of relatively massive BBHs like GW150914. We also derive a simple scaling relation for the expected number of detectable Galactic BBHs.

  10. COMPARISON OF ELISA AND IFA FOR ESTIMATION OF ANTIBODY LEVELS OF CATTLE TO THEILERIA ANNULATA VACCINE

    Directory of Open Access Journals (Sweden)

    Fariba Golcliiiifar

    2003-06-01

    Full Text Available Bovine tropical Theileriosis caused by Theileria annulata is an economically important disease of cattle. An enzyme linked immunosorbent assay (ELISA was used to determine antibody levels in vaccinated and unvaccinated cattle, us¬ing cellular schizont as antigen and its results were compared with immunofluo-rescent assay (IFA. For this test 126 sera collected (105 vaccinated, 31 not vacci¬nated from cows and assayed with ELISA which among them 104 sera were positive and 32 sera were negative. Same sample assayed with IFA in which 99 were positive sera and 37 were negative sera. Thereby the sensitivity and speci¬ficity of this ELISA on comparsion with IFA were 95.5% and 66.6% respectively. This study revealed that ELISA could be successfully used for both differentiat¬ing vaccinated and not vaccinated cattle and obtaining the titer of vaccinated cattle.

  11. Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA

    Directory of Open Access Journals (Sweden)

    Alexandra H. Heussner

    2010-06-01

    Full Text Available A monoclonal antibody specific to ochratoxin B (OTB was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD for OTB of 3 µg/L (8 nM, a limit of quantification (LOQ of 3.7 µg/L (10 nM, and a 50% inhibitory concentration (IC50 of 150 nM. Due to very low cross-reactivity to OTA (2.7% and structurally related molecules (0%, this OTB-ELISA was found to be suitable to detect OTB with excellent precision in different matrices, i.e., beer, coffee and wine. Therefore, this OTB-ELISA will allow screening of OTB in food and feed products.

  12. Establishment of PCR-ELISA for Detecting Glyphosate Resistant Transgenic Soybean

    Institute of Scientific and Technical Information of China (English)

    Yuan Qiang; Wei Yun-min; Fu Ming-ming; Qiu You-wen; Wen Hong-tao; Zhang Ming-hui; Liu Ying; Ao Jin-xia

    2016-01-01

    A PCR-ELISA method for detecting the glyphosate resistant transgenic soybean was established and optimized. The results showed that the key parameters of PCR-ELISA were as follows: the concentration of digoxin tag probe was 0.5 μmol• L-1, the time of hybridization reaction was 15 min and the chromogenic reaction should last for 30 min. The sensitivity and the repeatability of our PCR-ELISA method were evaluated, and the results showed that it could be detected when the concentration of DNA template from transgenic soybean samples was 0.01% or higher, and the coefficient of variation of this method was less than 5% in our research condition. These results suggested that PCR-ELISA method establishment in this study had good repeatability and high precision for detecting the transgenic soybean samples.

  13. Indirect ELISA with Recombinant GP5 for Detecting Antibodies to Porcine Reproductive and Respiratory Syndrome Virus

    Institute of Scientific and Technical Information of China (English)

    Yan Chen; Hong Tian; Jian-Hui He; Jin-Yin Wu; You-jun Shang; Xiang-tao Liu

    2011-01-01

    Porcine reproductive and respiratory syndrome is caused by the PRRS virus(PRRSV), which has six structural proteins(GP2, GP3, GP4, GP5, M and N). GP5 and N protein are important targets for serological detection by enzyme-linked immunosorbent assay(ELISA)and other methods. Toward this goal, we developed an indirect ELISA with recombinant GP5 antigens and this method was validated by comparison to the LSI PRRSV-Ab ELISA kit. The results indicated that the optimal concentration of coated recombinant antigen was 0.2 μg/well for a serum dilution of 1:40. The rate of agreement with the LSI PRRSV-Ab kit was 88.7%(266/300). These results support the potential use of recombinant GP5 as an antigen for indirect ELISA to detect PRRSV antibodies in pigs.

  14. The design of wavelength selector for full-automatic ELISA analyzer

    Science.gov (United States)

    Bao, Yan; Dong, Mingli; Zhu, Lianqing; Chang, Haitao; Li, Hong

    2011-05-01

    In recent years, ELISA technology has been developed rapidly and full-automatic ELISA analyzer, which is of significant practical value is widely used in the diagnosis of many diseases, such as, bacteria and viruses. Optical detection system is the hard core of fully automated ELISA analyzer and the key part of system is high-precision wavelength selector. The authors in this paper present the design of wavelength selector composed of light source, optical circuit plan, color filters and the module of signal acquisition. A control system for stepper motor which is used to choose the suitable color filters based on the microcontroller 8051 is introduced. From the results of experiment test, it can be seen that the wavelength selector is sufficient to meet the requirements of the full-automatic ELISA analyzer.

  15. A liquid phase blocking ELISA for the detection of antibodies against infectious bronchitis virus

    Directory of Open Access Journals (Sweden)

    Cardoso T.C.

    1999-01-01

    Full Text Available A liquid phase blocking ELISA (LPB-ELISA was developed for the detection and measurement of antibodies against infectious bronchitis virus (IBV. The purified and nonpurified virus used as antigen, the capture and detector antibodies, and the chicken hyperimmune sera were prepared and standardized for this purpose. A total of 156 sera from vaccinated and 100 from specific pathogen-free chickens with no recorded contact with the virus were tested. The respective serum titers obtained in the serum neutralization test (SNT were compared with those obtained in the LPB-ELISA. There was a high correlation (r2 = 0.8926 between the two tests. The LPB-ELISA represents a single test suitable for the rapid detection of antibodies against bronchitis virus in chicken sera, with good sensitivity (88%, specificity (100% and agreement (95.31%.

  16. Inkassofirma ähvardab tuhandeid Elisa kliente aegunud nõuetega / Lauri Linnamäe

    Index Scriptorium Estoniae

    Linnamäe, Lauri

    2008-01-01

    Julianus Inkasso nõuab Elisa praegustelt ja kunagistelt klientidelt aegunud võlgade tasumist koos intressidega. Kommenteerib Urmas Volens. Vt. samas: Julianus Inkasso: aegumist saab tõlgendada mitmeti

  17. The use of the antigen ELISA for monitoring tsetse and trypanosomosis control programmes in Zimbabwe

    International Nuclear Information System (INIS)

    Blood and serum samples from cattle originating from tsetse free and tsetse infested areas were analyzed using the Buffy Coat technique and an ELISA to detect trypanosomes and trypanosomal antigens, respectively. The results of the two tests were compared and apparent sensitivity and trypanosomal prevalence were calculated. The BCT seemed to be the most suitable test to detect acute infections, while the antigen capture ELISA (Ag-ELISA) was able to detect more chronic infections. The specificity of the Ag-ELISA was found to be very good, but the sensitivity of the test should be improved. One way to detect more T. congolense and T. vivax infections was to lower the cut-off point of percent positivity from 10 to 5%. (author). 1 ref., 1 fig., 7 tabs

  18. Prospects of eLISA for Detecting Galactic Binary Black Holes Similar to GW150914

    CERN Document Server

    Seto, Naoki

    2016-01-01

    We discuss the prospects of eLISA for detecting gravitational waves (GWs) from Galactic binary black holes (BBHs) similar to GW150914. For a comoving merger rate that is consistent with current observation, eLISA is likely to identify at least one BBH with a sufficient signal-to-noise ratio. In addition, eLISA has a potential to measure the eccentricity of the BBH as small as $e\\sim 0.05$, corresponding to the residual value $e\\sim 10^{-6}$ at 10Hz. Therefore, eLISA could provide us with a crucial information to understand the formation processes of relatively massive BBHs like GW150914. We also derive a simple scaling relation for the expected number of detectable Galactic BBHs.

  19. Parts Per Trillion Detection of 7-Aminonitrazepam by Nano-Enhanced ELISA

    Directory of Open Access Journals (Sweden)

    Feng Xue

    2013-09-01

    Full Text Available It is challenging to detect 7-aminonitrazepam (7-ANZP residue in animal tissues simply and sensitively by the enzyme-linked sorbent immunoassay (ELISA method. This paper demonstrates that utilizing a bioconjugate of gold nanoparticles and enzyme-labeled antibody as a signal probe increases the sensitivity of a traditional ELISA for 7-ANZP by nearly 20 times. The sensitivity of this ELISA for 7-ANZP was 5.6 pg/mL in buffer, and the limit of detection (LOD of 0.18 µg/kg for 7-ANZP in urine could be achieved after the urine samples were simply hydrolyzed and diluted by buffer. This simple and sensitive method has potential application for improving the sensitivity of ELISA methods against various small molecules.

  20. Monoclonal antibodies against proteins of the IBR virus nucleocapside and their assessing by ELISA

    Directory of Open Access Journals (Sweden)

    Jeannette Navarrete 0.

    2011-12-01

    Full Text Available Monoclonal antibodies were produced against the nucleocapside of a field IBR virus strain. The virus was growth in MOBK cell line. Viral components were purified and concentrated in a continuous 30% sacarose gradient followed by chemical precipitation. Nucleocapsides were run in a 10% SDS-PAGE gel. Positive hybridomes were tested using a direct ELISA developed in our laboratory. As a result eigth ELISA positive clones were obtained, from these five were also positive to seronetralization and immunodot. The clones recognize a 39.8Kda protein. Aditionally, a capture-ELISA was developed using the monoclonal antobodies from this research. This ELISA is useful to detect a reference as a field nm virus strain.

  1. Bepaling van het gehalte aan soja in vleesprodukten m.b.v. ELISA-methode

    NARCIS (Netherlands)

    Visser-Meijer, M.A.; Buizer, F.G.

    1983-01-01

    Het gehalte soja-eiwit is bepaald in 3 monsters vleesprodukt met bekende hoeveelheid soja en vervolgens in 7 monsters vleesprodukt met onbekende hoeveelheid soja. De bepaling geschiedde met behulp van een ELISA methode.

  2. ELISA and the Joint FAO/IAEA Division: Accomplishments and opportunities

    International Nuclear Information System (INIS)

    The enzyme linked immunosorbent assay (ELISA) is an inexpensive yet sensitive and specific tool in the diagnosis of livestock diseases. The use of the ELISA can be associated with many efforts at reducing the enormous wastage of animals caused by infectious diseases. The use of this technology in the developing countries was recommended in 1983 by a consultants meeting called by the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna. Since then, enormous resources have been committed towards the development and standardization of ELISA kits and their application, mostly in Africa, Asia and Latin America. The IAEA introduced training programmes, initiated regional research efforts, sponsored technical co-operation projects, provided ELISA readers and other equipment and dispatched experts/trainers to assist laboratories in developing countries. International standards for ELISA data expression and evaluation, reagent reference standards and an external quality assurance programme have been developed with assistance from consultants so that laboratories can meet minimal ELISA performance standards. Despite these efforts, the proper validation of ELISAs for application in some geographical areas is still difficult. Unique specificity problems occur because of unknown substances in sera of test animals. The application of ELISA in the field is difficult, and usually laboratories are required for the analysis of samples. Even in the laboratory the technique is easily 'abused', resulting in invalid data. Therefore, the ELISAs of the future that will be most effective in control and eradication of livestock diseases in developing countries should be simpler, more specific, less prone to error and portable for use in remote areas. They will probably consist of membrane based ELISA technologies accompanied by handheld portable touch screen computers for data management. Inexpensive means of identification of tested animals are becoming available

  3. Antigenisitas, Sensitivitas, dan Spesifisitas Protein Toxocara canis pada Pemeriksaan Antibodi Serum Mencit dengan Indirect-ELISA

    OpenAIRE

    Sri Subekti Bendryman; Kusnoto .; Tutik Juniastuti

    2015-01-01

    The aim of this research were to determine antigenicity, sensitivity, and specificity of Toxocara canisprotein used as antigen in indirect-ELISA for the detection antibody against the worm in the infected hostin order to proper diagnose kit. The design used was true experimental, with Post-test Only ControlGroups Design. Mouse was immunized with various worm homogenates used to antigenicity, sensitivityand specificity tests of T. canis protein with indirect-ELISA technique. The independence v...

  4. Comparison of E and NS1 antigens capture ELISA to detect dengue viral antigens from mosquitoes

    Directory of Open Access Journals (Sweden)

    Day-Yu Chao

    2015-01-01

    Interpretation & conclusion: With the future potential of antigen capture ELISA to be used in the resource deprived regions, the study showed that E-ELISA has similar sensitivity and antigen stability as NS1 Ag kit to complement the current established virological surveillance in human. The improvement of the sensitivity in detecting DENV-3/4 will be needed to incorporate this method into routine mosquito surveillance system.

  5. Refining the LPS-Antigen in Salmonella Antibody Elisa for Poultry Enhanced Specificity without Impairing Sensitivity

    DEFF Research Database (Denmark)

    Lauritsen, Klara Tølbøl; Lind, Peter; Klausen, Joan;

    2014-01-01

    In the Danish serological surveillance for Salmonella in poultry (serum and egg yolk) a mix-ELISA is used, based on S. typhimurium and S. enteritidis antigens (Feld et al., 2000). When we evaluated results of the test retrospectively, over the years an unacceptably large fraction of seropositive...... findings could not be confirmed by the subsequent confirmatory bacteriological sampling in the herd. Therefore we tried to enhance specificity of the ELISA, without losing sensitivity, by refining the antigens used....

  6. Comparison of ELISA, SPACE, and electron microscopy for the routine diagnosis of rotavirus infection.

    OpenAIRE

    Wall, R A; Mellars, B J; Luton, P.; Boulding, S

    1982-01-01

    Previous studies on the serological diagnosis of rotavirus infection have utilised locally produced antibodies. In this study we have compared two commercially produced assays, an ELISA (Rotazyme, Abbott) and a newly developed assay--solid phase aggregation of coupled erythrocytes (SPACE) (Wellcome Research Laboratories), with electron microscopy (EM). The SPACE test appeared less sensitive than EM. The ELISA was shown to be as sensitive as EM but more versatile. Our experience suggests that ...

  7. Application of ELISA for diagnosing and investigating the epidemiology of trypanosomiasis in buffaloes in north Vietnam

    International Nuclear Information System (INIS)

    An antigen-detection enzyme immunoassay (Ag-ELISA) based on a Trypanosoma evansi specific monoclonal antibody was used for the detection of circulating antigens in experimentally infected buffaloes and in buffaloes kept in different geographical regions of Vietnam. In experimental infections, circulating antigens were detected around 7 to 14 days after infection but the levels detected were considerably lower than those in sera collected from the field, suggesting that the parasite isolate used became highly adapted to mice thorough passage. In a study of field sera the agreement between the Ag-ELISA and parasitological methods for detecting infected cases was 80% and the apparent specificity of the test was 90%. In these and other studies the Ag-ELISA consistently detected many more infected cases than did the conventional parasitological methods. In most cases animals whose sera reacted in the Ag-ELISA also had trypanosome specific antibodies in their circulation. Treatment of infected animals with a trypanocidal drug in most cases resulted in a significant drop in trypanosome antigen levels but their status with respect to trypanosomal antibodies remained unchanged. Seroepidemiological surveys using Ag-ELISA and Ab-ELISA and base on an analysis of around 1000 sera collected from different regions of Vietnam demonstrated prevalence ranging between 17% and 50% with no apparent relationship to geographical region. However, prevalence of infection was generally higher in summer (rainy season), when fly activity is highest. It is concluded that serological methods based on Ag-ELISA and Ab-ELISA are valuable adjuncts to clinical and parasitological methods for diagnosing trypanosomiasis, studying the epidemiology of the disease and monitoring the effectiveness of control programmes. (author). 12 refs, 2 figs, 3 tabs

  8. Standardization of dot-ELISA for the serological diagnosis of toxocariasis and comparision of the assay with ELISA Padronização do teste dot-ELISA para o diagnóstico da toxocaríase, estudo comparativo com o teste imunoenzimático ELISA

    Directory of Open Access Journals (Sweden)

    Eide Dias Camargo

    1992-02-01

    Full Text Available The dot-enzyme-linked immunosorbent assay (dot-ELISA was standardized using somatic (S and excretory-secretory (ES antigens of Toxocara-canis for the detection of specific antibodies in 22 serum samples from children aged 1 to 15 years, with clinical signs of toxocariasis. Fourteen serum samples from apparently normal individuals and 28 sera from patients with other pathologies were used as controls. All samples were used before and after absorption with Ascaris suum extract. When the results were evaluated in comparison with ELISA, the two tests were found to have similar sensitivity, but dot-ELISA was found to be more specific in the presence of the two antigens studied. Dot-ELISA proved to be effective for the diagnosis of human toxocariasis, presenting advantages in terms of yield, stability, time and ease of execution and low cost.Padronizou-se o teste imunoenzimático dot-ELISA, empregando-se os antígenos somático (S e excretor-secretor (ES de Toxocara canis, para pesquisa de anticorpos específicos em 22 soros de pacientes com idades entre 5 a 15 anos, com dados clínicos de toxocaríase. Como grupo controle, foram estudados 14 soros de indivíduos supostamente normais e 28 soros de pacientes com outras patologias. Todas as amostras em estudo foram empregadas antes e após absorção com extrato de Ascaris suum. Os resultados obtidos foram avaliados comparativamente com o teste ELISA evidenciando, nos dois testes estudados, comportamento semelhante quanto à sensibilidade e maior especificidade para o dot-ELISA, com qualquer dos dois antígenos estudados. O teste dot-ELISA mostrou-se eficiente para o diagnóstico da toxocaríase humana, apresentando vantagens quanto ao rendimento, estabilidade, tempo e facilidade de execução e baixo custo.

  9. Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever

    OpenAIRE

    Paredes Julio Cesar Muñoz; Oliveira Liliane Guimarães; Braga Alexandre de Carvalho; Trevisol Iara Maria; Roehe Paulo Michel

    1999-01-01

    An indirect enzyme linked immunoassay (ELISA-I) was developed and standardized for the serological diagnosis of classical swine fever (CSF). For the comparison, nine hundred and thirty-seven swine serum samples were tested by serum neutralization followed by immunoperoxidase staining (NPLA), considered as the standard. Of these, 223 were positive and 714 negative for neutralizing antibodies to classical swine fever virus (CSFV). In relation to the NPLA, the ELISA-I presented a 98.2% sensitivi...

  10. ELISA assays for the detection of Bothrops lanceolatus venom in envenomed patient plasmas.

    Science.gov (United States)

    Rodriguez-Acosta, A; Uzcategui, W; Azuaje, R; Giron, M E; Aguilar, I

    1998-01-01

    A double antibody sandwich enzyme linked immunosorbant assay (ELISA) was carried out to detect Bothrops Ianceolatus venom in plasma from envenomed patients at various time intervals (0, 6, 12, 18 and 24 hrs). The test could detect Bothrops lanceolatus levels up to 12 ng/mL of envenomed patient plasmas. Elaboration of an easy, fast and species-diagnostic based on this ELISA technique useful to physicians is discussed. PMID:11845439

  11. Implicaciones de la zona Ossa-Morena y dominios equivalentes en el modelo geodinámico de la Cadena Hercinica europea

    Directory of Open Access Journals (Sweden)

    Castro, A.

    1987-08-01

    Full Text Available The Ossa-Morena zone (OMZ has some peculiar features clearly different from those of the other inner domains in the Iberic Hercynian chain. It is a continental domain (microcontinent ? attached, from Lower Paleozoic times, to the continental crust of the Central Iberian zone (CIZ. During the Hercynian collision (Upper Paleozoic the OMZ behaved in a way different form that of adjacent areas (CIZ due to the peculiar features (Iess thicknees and pre-Hercynian evolution (Ordovician rifting. The OMZ, and their equivalent domains in the Iberian peninsula as well as in European chain, can have strong implications on the evolution of the Hercynian orogene. Part of the OMZ crust (and equivalent domains, together with the oceanic crust developed within it during the Ordovician rifting, was obduced over the inner domains (CIZ in areas of frontal collision (e.g. NW Iberian peninsula and French Massif Central and strongly stretched in areas of oblique collision (e.g. OMZ of SW Iberian peninsula and North Armorican domains.La zona Ossa-Morena (ZOM del hercínico peninsular posee unas características peculiares que la diferencian netamente de los otros dominios internos de la Cadena. Se trata de un dominio cortical (microcontinente ? adosado a la corteza de la Zona Centro-Ibérica (ZCI desde el Paleozoico Inferior. Durante la colisión continental hercínica (Palezoico Superior la ZOM responde de diferente forma que las zonas adyacentes (ZCI debido a su diferente constitución (menor espesor y peculiar evolución prehercínica (rifting ordovícico. Esta zona y sus equivalentes en otros dominios del hercínico peninsular y europeo, tienen fuertes implicaciones en el desarrollo del orógeno hercínico. Parte de la corteza continental de la ZOM fue, junto con la corteza oceánica desarrollada localmente en su interior durante el rifting ordovícido, obducida sobre los dominios internos (ZCI en zonas de colisión frontal (e.g. NW peninsular y Macizo C

  12. Diagnóstico ecológico y económico de la cadena de suministros para el reciclaje de plásticos en el contexto empresarial cubano

    Directory of Open Access Journals (Sweden)

    Michael Feitó Cespón

    2015-01-01

    Full Text Available La cadena de suministros para el reciclaje de plásticos en Cuba ha estado presentando un bajo desempe ̃ no durante varios a ̃ nos, por lo tanto el objetivo de este trabajo es realizar un diagnóstico estratégico de su gestión que integre las dimensiones económicas y medioambientales. Para conseguir este propósito se utilizan los procesos de pensamiento desarrollados dentro de la teoría de las restricciones los cuales se fortalecen con el análisis del ciclo de vida, técnicas estadísticas y métodos multicriterios discretos. Los resultados del diagnóstico demostraron la necesidad de redise ̃ nar las decisiones estratégicas teniendo en cuenta los criterios económicos integrados con los medioambientales con el fin de lograr un desempe ̃ no sostenible de la cadena de suministros estudiada.

  13. El nodo de distribución en la cadena hortícola de valor México-Estados Unidos: el caso de las exportaciones sinaloenses de productos frescos

    Directory of Open Access Journals (Sweden)

    Carlos Javier Maya Ambía

    2009-01-01

    Full Text Available Sinaloa es la principal entidad federativa exportadora de hortalizas frescas de México a Estados Unidos. Su inserción en la globalización ocurre a través de la cadena hortícola de valor que existe entre ambos países. Para que ésta funcione de manera eficiente, es central el papel de las empresas que distribuyen en Estados Unidos las hortalizas producidas en Sinaloa. Después de explicar las características de la cadena en el contexto del mercado hortícola mundial, a partir del trabajo de campo realizado en la zona fronteriza de Nogales, Arizona, el presente artículo analiza la experiencia de los horticultores sinaloenses que crearon distribuidoras propias en territorio estadounidense, para mejorar su posición competitiva en el mercado y tener mayor control sobre sus productos. Asimismo, se detalla la situación actual y los desafíos que la globalización impone a las empresas estudiadas.

  14. Constraining stellar binary black hole formation scenarios with eLISA eccentricity measurements

    CERN Document Server

    Nishizawa, Atsushi; Berti, Emanuele; Klein, Antoine

    2016-01-01

    A space-based interferometer such as eLISA could observe few to few thousands progenitors of black hole binaries (BHBs) similar to those recently detected by Advanced LIGO. Gravitational radiation circularizes the orbit during inspiral, but some BHBs retain a measurable eccentricity at the low frequencies where eLISA is most sensitive. The eccentricity of a BHB carries precious information about its formation channel: BHBs formed in the field, in globular clusters, or close to a massive black hole (MBH) have distinct eccentricity distributions in the eLISA band. We generate mock eLISA observations, folding in measurement errors, and using Bayesian model selection we study whether eLISA measurements can identify the BHB formation channel. We find that a handful of observations would suffice to tell whether BHBs were formed in the gravitational field of a MBH. Conversely, several tens of observations are needed to tell apart field formation from globular cluster formation. A five-year eLISA mission with the lon...

  15. Comparative detection of bacterial adhesion to Caco-2 cells with ELISA, radioactivity and plate count methods.

    Science.gov (United States)

    Le Blay, Gwenaëlle; Fliss, Ismaïl; Lacroix, Christophe

    2004-11-01

    Different methods are used to study bacterial adhesion to intestinal epithelial cells, which is an important step in pathogenic infection as well as in probiotic colonization of the intestinal tract. The aim of this study was to compare the ELISA-based method with more conventional plate count and radiolabeling methods for bacterial adhesion detection. An ELISA-based assay was optimized for the detection of Bifidobacterium longum and Escherichia coli O157:H7, which are low and highly adherent bacteria, respectively. In agreement with previous investigations, a percentage of adhesion below 1% was obtained for B. longum with ELISA. However, high nonspecific background and low positive signals were measured due to the use of polyclonal antibodies and the low adhesion capacity with this strain. In contrast, the ELISA-based method developed for E. coli adhesion detected a high adhesion percentage (15%). For this bacterium the three methods tested gave similar results for the highest bacterial concentrations (6.8 Log CFU added bacteria/well). However, differences among methods increased with the addition of decreased bacterial concentration due to different detection thresholds (5.9, 5.6 and 2.9 Log CFU adherent bacteria/well for radioactivity, ELISA and plate count methods, respectively). The ELISA-based method was shown to be a good predictor for bacterial adhesion compared to the radiolabeling method when good quality specific antibodies were used. This technique is convenient and allows handling of numerous samples.

  16. Development of an enzyme-linked immuno-sorbent assay (ELISA) method for carbofuran residues.

    Science.gov (United States)

    Yang, Jinyi; Wang, Hong; Jiang, Yueming; Sun, Yuanming; Pan, Ke; Lei, Hongtao; Wu, Qing; Shen, Yudong; Xiao, Zhili; Xu, Zhenlin

    2008-04-17

    The haptens 4-[[(2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy)carbonyl]-amino]butanoic acid (BFNB) and 6-[((2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy)-carbonylamino]hexanoic acid (BFNH) were synthesized and then used to develop a rapid,specific and sensitive ELISA method to determine residues of the pesticide carbofuran in a variety of matrices. A hybridoma cell line (5D3) producing anti-carbofuran monoclonal antibodies (MAbs) was also established. Based on the MAbs in combination with the heterologous hapten BFNH coupled to either horseradish peroxidase (HRP) or ovalbumin(OVA), four ELISAs (formats I-IV) for the quantification of carbofuran were developed and compared. Among them, the optimized format II (the conjugate-coated direct competitive ELISA) showed the best characteristics, with an IC50 value of 18.49 ng/mL, a limit of detection of 0.11 ng/mL and the shortest assay time (1 h). This ELISA method was then applied to the determinations of carbofuran in environmental water, soil and food samples. The relative standard deviations (R.S.D.s) ranged from 1.8% to 21.3% and the mean recoveries were 104.6%, 108.3%, 106.3% and 100.1% for water, soil, lettuce and cabbage, respectively. Thus, the ELISA method of format II exhibited the potential to develop commercial ELISA kits for a rapid detection of carbofuran for human health and environmental safety.

  17. Development of an Enzyme-linked Immuno-Sorbent Assay (ELISA Method for Carbofuran Residues

    Directory of Open Access Journals (Sweden)

    Zhenlin Xu

    2008-04-01

    Full Text Available The haptens 4-[[(2,3-dihydro-2,2-dimethyl-7-benzofuranyloxycarbonyl]-amino]butanoic acid (BFNB and 6-[((2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy-carbonylamino]hexanoic acid (BFNH were synthesized and then used to develop a rapid,specific and sensitive ELISA method to determine residues of the pesticide carbofuran in avariety of matrices. A hybridoma cell line (5D3 producing anti-carbofuran monoclonalantibodies (MAbs was also established. Based on the MAbs in combination with theheterologous hapten BFNH coupled to either horseradish peroxidase (HRP or ovalbumin(OVA, four ELISAs (formats I-IV for the quantification of carbofuran were developedand compared. Among them, the optimized format II (the conjugate-coated directcompetitive ELISA showed the best characteristics, with an IC50 value of 18.49 ng/mL, alimit of detection of 0.11 ng/mL and the shortest assay time (1 h. This ELISA method wasthen applied to the determinations of carbofuran in environmental water, soil and foodsamples. The relative standard deviations (R.S.D.s ranged from 1.8% to 21.3% and themean recoveries were 104.6%, 108.3%, 106.3% and 100.1% for water, soil, lettuce andcabbage, respectively. Thus, the ELISA method of format II exhibited the potential todevelop commercial ELISA kits for a rapid detection of carbofuran for human health andenvironmental safety.

  18. Validation of an ELISA for the quantitation of lanoteplase, a novel plasminogen activator.

    Science.gov (United States)

    Stouffer, B; Habte, S; Vachharajani, N; Tay, L

    1999-11-01

    An ELISA was developed and validated for the quantitation of lanoteplase in human citrated plasma. The ELISA employed a monoclonal anti-lanoteplase antibody absorbed onto 96-well microtiter plates to capture lanoteplase in citrated human plasma samples containing PPACK, a protease inhibitor. The captured lanoteplase was detected using a biotinylated rabbit anti-lanoteplase polyclonal antibody. The standard curve range in human plasma for the ELISA was 7-100 ng/ml. Assessment of individual standard curve variability indicated reproducible responses with r2 values of > or = 0.985. The accuracy (% DEV) and precision (%RSD) estimates for the ELISA based on the predicted values from quality control (QC) samples were within 7.3% and 11%, respectively. Cross-reactivity with t-PA was determined to be less than 11% by ELISA. The stability of lanoteplase was established in human citrated PPACK plasma for 24 hours at 4 degrees C, for 2 months at -20 degrees C, for 22 months at -70 degrees C, three weeks at room temperature, and through four freeze/thaw cycles. To quantify lanoteplase plasminogen activator (PA) activity, a commercially available chromogenic activity assay was also validated. This method and its application is described briefly here. The lanoteplase ELISA as well as the commercial activity method were successfully employed to evaluate the pharmacokinetic parameters of lanoteplase in support of clinical Phase II/III studies. PMID:10595857

  19. RT-PCR-ELISA as a tool for diagnosis of low-pathogenicity avian influenza

    DEFF Research Database (Denmark)

    Dybkær, Karen; Munch, Mette; Handberg, Kurt;

    2003-01-01

    A one-tube reverse transcriptase/polymerase chain reaction coupled with an enzyme-linked immunosorbent assay (RT-PCR-ELISA) was developed for the rapid detection of avian influenza virus (AIV) in clinical specimens. A total of 419 swab pools were analyzed from chickens experimentally infected wit...... of the twenty-three VI-positive specimens were negative when tested by RT-PCR-ELISA. The diagnostic sensitivity and specificity of the RT-PCR-ELISA was 91% and 97%, respectively, using VI in SPF eggs as the gold reference standard.......A one-tube reverse transcriptase/polymerase chain reaction coupled with an enzyme-linked immunosorbent assay (RT-PCR-ELISA) was developed for the rapid detection of avian influenza virus (AIV) in clinical specimens. A total of 419 swab pools were analyzed from chickens experimentally infected...... with low-pathogenicity AIV, from wild aquatic birds, and from domestic ducks. The AIV was detected in 32 swab pools by RT-PCR-ELISA compared to 23 by virus isolation (VI) in embryonated specific pathogen free (SPF) chicken eggs. Thus, 39% more specimens were positive by RT-PCR-ELISA than by VI. Two...

  20. Evaluation of serum and milk ELISAs for paratuberculosis in Danish dairy cattle

    DEFF Research Database (Denmark)

    Klausen, Joan; Huda, A.; Ekeroth, Lars;

    2003-01-01

    A milk and a serum ELISA for detection of antibodies against Mycobacterium avium ssp. paratuberculosis (MAP) were evaluated against the complement-fixation test (CFT) and culture of faecal samples from 580 cows collected between August 1996 and December 1996. Milk and serum were obtained...... concurrently from six dairy herds infected with MAP and from two dairy herds without history of infection with MAP. A cut-off value of 7 OD% was used in the ELISAs. At this cut-off value, all six culture-positive herds were positive in the serum ELISA but one was negative in the milk ELISA. All six culture......-positive herds were positive in the CFT. In the two culture-negative herds, the serum and the milk ELISA deemed all serum samples negative at this cut-off value, whereas four serum samples from one of these herds were positive in the CFT. The highest cut-off value enabling the milk ELISA to record all six...

  1. Development of an ELISA using anti-idiotypic antibody for diagnosis of opisthorchiasis.

    Science.gov (United States)

    Bulashev, Aitbay K; Borovikov, Sergey N; Serikova, Shynar S; Suranshiev, Zhanbolat A; Kiyan, Vladimir S; Eskendirova, Saule Z

    2016-01-01

    Monoclonal antibody specific for an epitope of cretory-secretory antigen protein of Opisthorchis felineus (Rivolta, 1884) (Trematoda: Opisthorchiidae) with a molecular weight of 28 kDa was used in a sandwich enzyme-linked immunosorbent assay (ELISA) for immobilisation of liver fluke specific antigen to the solid phase. Examination of human sera by this ELISA compared with commercial assays demonstrated that the monoclonal antibody epitope is located within this significant parasite protein. Anti-idiotypic antibody specific for the paratope of this monoclonal antibody was obtained by a hybridoma technique. Mimicking an epitope of excretory-secretory antigen of O. felineus, it had the capacity to bind specific antibody and elicit an antibody response. The value of anti-idiotypic antibody as a substitute for the liver fluke antigen was tested by ELISA using serum samples of infected dogs. Anti-idiotypic antibody proved to be of value in both an indirect-ELISA and a competitive-ELISA for diagnosis of opisthorchiasis. Mature trematodes were isolated from all infected animals. The faecal egg counts were negative in dogs with a relatively small number of parasites, despite finding antibodies in serum by ELISA. Substitution of parasite antigen with anti-idiotype avoids the use of experimental animals and also reduces time-consuming steps of antigen preparation. PMID:27507639

  2. Validation and quality control of ELISAs for the use with human saliva samples.

    Science.gov (United States)

    Jaedicke, Katrin M; Taylor, John J; Preshaw, Philip M

    2012-03-30

    Enzyme-linked immunosorbent assays (ELISAs) have proven to be a powerful tool for fast and reliable sample analysis, in both clinical diagnostics and in research. Most assays are now available for use with a range of different analytical fluids, including serum, plasma or urine. In recent years, saliva has drawn attention as a potentially valuable diagnostic fluid; however few ELISAs have been validated for use with saliva, or their validation is often incomplete. Saliva has a number of different physical characteristics than, for example, cell culture medium or serum and assuming an ELISA which works well with serum samples will also do so with saliva potentially could lead to erroneous data and conclusions. In this report, we provide a detailed protocol to validate any ELISA for use with saliva samples and show the results of validation procedures for 13 ELISAs for using saliva. Our findings suggest that the majority of ELISAs work reliably with saliva, even if the assay was not specifically designed for this biological fluid. However, we also report a few cases where recovery or intra-and inter-assay variations were unexpectedly high, emphasising the importance of performing a validation procedure for each assay before using it with saliva to ensure accurate and reliable data.

  3. Development of an ELISA Method Detecting Strobilurin Fungicide through the Immune Magnetic Beads Collecting Samples

    Directory of Open Access Journals (Sweden)

    Xun C. Huang

    2015-02-01

    Full Text Available Trifloxystrobin, one of the strobilurin fungicides, is active as inhibitor of low toxicity and easy degradation against a wide range of fungal plant pathogens. In the experiment, Hydroxy from magnetic microsphere and amino of anti-Trifloxystrobin antibody could condensate to form Immune Magnetic Beads (IMB, which could specially capture trifloxystrobin in the food matrix and rapidly separated it through the magnetic field. An ELISA method (IMB-ELISA was developed to detect the captured fungicide through examining the best reactive concentration, sensitivity, specificity, matrix effect and recovery rate. The half maximal inhibitory concentration (IC50 value obtained by the IMB-ELISA method was 2.28 &mug/mL (R2 = 0.9837 against 12.57 &mug/mL of conventional ELISA method (R2 = 0.9747. The recovery rates were 90.12-103.65% and the Coefficients of Variation (CVs were in the range of 4.5-11.2%. In three kind of citrus samples, there was only low cross-reactivity in IMB-ELISA method by detecting matrix effects. Based on above experiment, the IMB-ELISA method was an efficient and sensitive method for Trifloxystrobin detection.

  4. The Use of Chicken Igy in a Double Antibody Sandwich Elisa for the Quantification of Melittin in Bee Venom and Bee Venom Melittin Content in Cosmetics

    OpenAIRE

    Suh Lindsey Y. K.; Kartoon Tayabaa; Gujral Naiyana; Yoon Youngmee; Suh Joo Won; Sunwoo Hoon

    2015-01-01

    Two enzyme-linked immunosorbent assay (ELISA) - based detection systems: indirect competitive ELISA and biotinylated double antibody sandwich ELISA (DAS-ELISA) were developed to determine the melittin concentration in honeybee (Apis mellifera) venom and the melittin concentration in cosmetics which contain bee venom. The indirect competitive ELISA employed chicken anti-melittin IgY. The biotinylated DAS-ELISA employed anti-melittin monoclonal antibody (MAb) and biotinylated anti-melittin IgY....

  5. Comparative Diagnosis of Serum IgG1 and Coproantigen ELISA for Fasciolosis Detection of Goats in Mexico.

    Science.gov (United States)

    Villa-Mancera, Abel; Molina-Mendoza, Pedro; Hernández-Guzmán, Karina; Olivares-Pérez, Jaime; Sarracent-Pérez, Jorge; Zumaquero-Ríos, José

    2016-01-01

    The objective of present study was to determine the prevalence of natural caprine fasciolosis in the Mixteca region of Mexico using coproantigen and serum IgG1 ELISA tests for comparative purposes. A total of 1070 serum and faecal samples were analyzed for IgG1 antibodies and coproantigens, using ELISA with E/S products as antigen and a monoclonal antibody-based sandwich ELISA. Prevalence of 73.46% was found using the serological ELISA and a percentage of 77.20 was found for coproantigen ELISA. The diagnostic sensitivity and specificity for serum ELISA were 86.7% and 96.4%, and for the coproantigen ELISA they were 93.1% and 97.8%, respectively. The seropositive samples were further categorized as low, medium, or high positivity. Results show a great proportion of low and medium positive goats when the serum ELISA test was used. Correlation coefficients between coproantigens and seropositivity were statistically significant (P < 0.01) for low seropositivity (r = 0.93) and medium seropositivity (r = 0.84). The accuracy of faecal antigen ELISA was higher compared to indirect ELISA serological test. Two ELISAs were shown to be useful for demonstrating the current status of F. hepatica infection in the endemic areas and can be employed in studies on epidemiology as well as anthelmintics treatment for preventing economic loss and the risk of transmission to humans. PMID:27563665

  6. Comparative Diagnosis of Serum IgG1 and Coproantigen ELISA for Fasciolosis Detection of Goats in Mexico.

    Science.gov (United States)

    Villa-Mancera, Abel; Molina-Mendoza, Pedro; Hernández-Guzmán, Karina; Olivares-Pérez, Jaime; Sarracent-Pérez, Jorge; Zumaquero-Ríos, José

    2016-01-01

    The objective of present study was to determine the prevalence of natural caprine fasciolosis in the Mixteca region of Mexico using coproantigen and serum IgG1 ELISA tests for comparative purposes. A total of 1070 serum and faecal samples were analyzed for IgG1 antibodies and coproantigens, using ELISA with E/S products as antigen and a monoclonal antibody-based sandwich ELISA. Prevalence of 73.46% was found using the serological ELISA and a percentage of 77.20 was found for coproantigen ELISA. The diagnostic sensitivity and specificity for serum ELISA were 86.7% and 96.4%, and for the coproantigen ELISA they were 93.1% and 97.8%, respectively. The seropositive samples were further categorized as low, medium, or high positivity. Results show a great proportion of low and medium positive goats when the serum ELISA test was used. Correlation coefficients between coproantigens and seropositivity were statistically significant (P < 0.01) for low seropositivity (r = 0.93) and medium seropositivity (r = 0.84). The accuracy of faecal antigen ELISA was higher compared to indirect ELISA serological test. Two ELISAs were shown to be useful for demonstrating the current status of F. hepatica infection in the endemic areas and can be employed in studies on epidemiology as well as anthelmintics treatment for preventing economic loss and the risk of transmission to humans.

  7. Comparative Diagnosis of Serum IgG1 and Coproantigen ELISA for Fasciolosis Detection of Goats in Mexico

    Directory of Open Access Journals (Sweden)

    Abel Villa-Mancera

    2016-01-01

    Full Text Available The objective of present study was to determine the prevalence of natural caprine fasciolosis in the Mixteca region of Mexico using coproantigen and serum IgG1 ELISA tests for comparative purposes. A total of 1070 serum and faecal samples were analyzed for IgG1 antibodies and coproantigens, using ELISA with E/S products as antigen and a monoclonal antibody-based sandwich ELISA. Prevalence of 73.46% was found using the serological ELISA and a percentage of 77.20 was found for coproantigen ELISA. The diagnostic sensitivity and specificity for serum ELISA were 86.7% and 96.4%, and for the coproantigen ELISA they were 93.1% and 97.8%, respectively. The seropositive samples were further categorized as low, medium, or high positivity. Results show a great proportion of low and medium positive goats when the serum ELISA test was used. Correlation coefficients between coproantigens and seropositivity were statistically significant (P<0.01 for low seropositivity (r=0.93 and medium seropositivity (r=0.84. The accuracy of faecal antigen ELISA was higher compared to indirect ELISA serological test. Two ELISAs were shown to be useful for demonstrating the current status of F. hepatica infection in the endemic areas and can be employed in studies on epidemiology as well as anthelmintics treatment for preventing economic loss and the risk of transmission to humans.

  8. Comparative Diagnosis of Serum IgG1 and Coproantigen ELISA for Fasciolosis Detection of Goats in Mexico

    Science.gov (United States)

    Molina-Mendoza, Pedro; Hernández-Guzmán, Karina; Olivares-Pérez, Jaime; Sarracent-Pérez, Jorge; Zumaquero-Ríos, José

    2016-01-01

    The objective of present study was to determine the prevalence of natural caprine fasciolosis in the Mixteca region of Mexico using coproantigen and serum IgG1 ELISA tests for comparative purposes. A total of 1070 serum and faecal samples were analyzed for IgG1 antibodies and coproantigens, using ELISA with E/S products as antigen and a monoclonal antibody-based sandwich ELISA. Prevalence of 73.46% was found using the serological ELISA and a percentage of 77.20 was found for coproantigen ELISA. The diagnostic sensitivity and specificity for serum ELISA were 86.7% and 96.4%, and for the coproantigen ELISA they were 93.1% and 97.8%, respectively. The seropositive samples were further categorized as low, medium, or high positivity. Results show a great proportion of low and medium positive goats when the serum ELISA test was used. Correlation coefficients between coproantigens and seropositivity were statistically significant (P < 0.01) for low seropositivity (r = 0.93) and medium seropositivity (r = 0.84). The accuracy of faecal antigen ELISA was higher compared to indirect ELISA serological test. Two ELISAs were shown to be useful for demonstrating the current status of F. hepatica infection in the endemic areas and can be employed in studies on epidemiology as well as anthelmintics treatment for preventing economic loss and the risk of transmission to humans. PMID:27563665

  9. Diagnosis of viral diseases using ELISA techniques: Current status and future prospects

    International Nuclear Information System (INIS)

    Use of enzyme-linked immunosorbent assays (ELISAs) for the detection of virus or anti-viral antibody has now become commonplace in diagnostic laboratories. These assays offer considerable advantages to the virologist over previously used tests because of their simplicity, lack of tissue culture requirements and ease of standardization. The direct ELISA is now seldom employed and indirect ELISAs using sandwich and competition procedures enable the versatility of ELISA to be fully exploited. Improvements in test procedures and reagents continue to increase the sensitivity and speed of the assays and utilization of monoclonal antibodies has resulted in greatly improved specificity with their use in competition assays, reducing the need for antigen purity. The versatility of new enzyme systems, with their increased sensitivity, improved conjugation and more distinctive colour changes, further increases the flexibility of this test system. In assay development and standardization use of sophisticated laboratory equipment is essential. The automatic ELISA microplate reader and high speed centrifuge are necessary tools for the correct establishment of a diagnostic ELISA. Once this step is completed standardized reagents are required in only small amounts and the test can be easily adapted for use in simply equipped laboratories using by-eye reading. This system of virus diagnosis has immense potential in the developing world. The cost and supply of reagents, difficulties of maintaining tissue culture and the complexity of equipment for other techniques for virus diagnosis are problems that have resulted in limited achievements in this area. ELISA offers a solution to most of these problems and perhaps its greatest prospect lies in the development of assays specifically designed for use in developing countries. (author)

  10. Development of a Sandwich ELISA for EHEC O157:H7 Intimin γ1.

    Science.gov (United States)

    Zhang, Xuehan; Li, Meng; Zhang, Bicheng; Chen, Kangming; He, Kongwang

    2016-01-01

    Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a zoonotic pathogen of worldwide importance that causes foodborne infections in humans. Intimin gamma 1 (intimin γ1) is one of the most important outer membrane proteins required for EHEC's intimate adhesion to epithelial cells. Here, we generated a polyclonal antibody (pAb) and a monoclonal antibody (mAb) against intimin γ1 to develop a double antibody sandwich ELISA (DAS-ELISA) with increased sensitivity and specificity for measuring EHEC O157:H7. To achieve this goal, a rabbit pAb was used as a capture antibody, and a mouse mAb was a detection antibody. No cross-reactivity was observed with the other genera of pathogenic bacteria tested with the DAS-ELISA, which included Salmonella enteritidis, Shigella flexneri type 2, Listeria monocytogenes, Streptococcus suis type 2, and other 18 serotype E. coli. Detection limits of the DAS-ELISA were 1 × 103 CFU/mL for EHEC O157:H7 cultures, 1 × 104 CFU/g before enrichment, and 1 × 102 CFU/g after enrichment of contaminated samples. Field samples (n = 498) were tested using a previously established duplex-PCR method and compared to our DAS-ELISA. The DAS-ELISA had a specificity of 94.4%, a sensitivity of 91.5% and accuracy of 94.0% compared with duplex-PCR. The DAS-ELISA developed here can be applied to EHEC O157:H7 quantification in food, animal, and environmental samples. PMID:27603508

  11. Validation of a phospholipase A2 receptor antibody ELISA in an Australian cohort with membranous glomerulonephritis.

    Science.gov (United States)

    Ong, Lawrence; Silvestrini, Roger; Chapman, Jeremy; Fulcher, David A; Lin, Ming Wei

    2016-04-01

    A commercial PLA2R Ab ELISA was validated by examining its ability to distinguish primary from secondary membranous nephropathy, correlating results with clinical markers of disease activity, and comparing its performance with an indirect immunofluorescence test (IIFT). PLA2R Ab levels were measured in 77 patients with biopsy proven membranous nephropathy, divided into either idiopathic (n = 61) or secondary groups (n = 6). In the idiopathic group, measures of contemporaneous disease activity (proteinuria, serum creatinine) were compared between seropositive and seronegative subjects. ELISA values were then compared with semi-quantitative results from an IIFT using PLA2R transfected HEK293 cells as substrate. The PLA2R Ab ELISA was positive in only 15 of 61 (25%) patients with idiopathic membranous nephropathy (IMN), but there was a significant negative relationship with time since diagnosis. Thus, in a subgroup of patients diagnosed within 6 months of analysis, the sensitivity was 6/15 (55%), rising to 6/8 (75%) in those recently-diagnosed patients who had not been treated. In the entire cohort, there was a significant positive correlation between ELISA values and degree of proteinuria, but our analysis did not control for variation of both variables with time. The PLA2R Ab ELISA also showed very high agreement with IIFT (96%). Therefore, the PLA2R Ab ELISA is a highly specific test for distinguishing primary from secondary membranous nephropathy that is most sensitive in newly diagnosed patients who have not received immunosuppression. Antibody levels correlated with degree of proteinuria, but this relationship was not shown to be independent of time. Both IIFT and ELISA platforms performed comparably.

  12. Development of a Sandwich ELISA for EHEC O157:H7 Intimin γ1

    Science.gov (United States)

    Li, Meng; Zhang, Bicheng; Chen, Kangming; He, Kongwang

    2016-01-01

    Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a zoonotic pathogen of worldwide importance that causes foodborne infections in humans. Intimin gamma 1 (intimin γ1) is one of the most important outer membrane proteins required for EHEC’s intimate adhesion to epithelial cells. Here, we generated a polyclonal antibody (pAb) and a monoclonal antibody (mAb) against intimin γ1 to develop a double antibody sandwich ELISA (DAS-ELISA) with increased sensitivity and specificity for measuring EHEC O157:H7. To achieve this goal, a rabbit pAb was used as a capture antibody, and a mouse mAb was a detection antibody. No cross-reactivity was observed with the other genera of pathogenic bacteria tested with the DAS-ELISA, which included Salmonella enteritidis, Shigella flexneri type 2, Listeria monocytogenes, Streptococcus suis type 2, and other 18 serotype E. coli. Detection limits of the DAS-ELISA were 1 × 103 CFU/mL for EHEC O157:H7 cultures, 1 × 104 CFU/g before enrichment, and 1 × 102 CFU/g after enrichment of contaminated samples. Field samples (n = 498) were tested using a previously established duplex-PCR method and compared to our DAS-ELISA. The DAS-ELISA had a specificity of 94.4%, a sensitivity of 91.5% and accuracy of 94.0% compared with duplex-PCR. The DAS-ELISA developed here can be applied to EHEC O157:H7 quantification in food, animal, and environmental samples. PMID:27603508

  13. Metodología basada en el Modelo de Referencia para Cadenas de Suministro para Analizar el Proceso de producción de Biodiesel a partir de Higuerilla Methodology based on the Supply Chain Operations Reference Model to Analyze the Production Process of Biodiesel from Castor Oil Plant

    Directory of Open Access Journals (Sweden)

    Fernando Salazar

    2012-01-01

    Full Text Available Se presenta un análisis para la producción de Biodiesel a partir de Higuerilla usando el Modelo de Referencia para Cadenas de Suministro (SCOR. Se identificaron los niveles del proceso, indicadores clave de desempeño, atributos y operaciones logísticas que se llevan a cabo en toda la cadena de la producción de biodiesel de higuerilla. La utilidad de la aplicación del modelo SCOR se determina por la identificación que logra de los componentes de la cadena, evidenciando fortalezas y debilidades que presenta en las relaciones externas e internas de la logística. Desde esta perspectiva se observó que la aplicación del modelo SCOR hace más eficiente las operaciones logísticas a lo largo de la cadena.The production process of Biodiesel from castor oil plants is analyzed using the Supply Chain Operations Reference Model (SCOR. The different levels of the process, the key performance indicators, the attributes and the logistic operations carried out throughout the chain were analyzed. The usefulness of applying the SCOR model is determined by the identification of the components of the chain, highlighting strengths and weaknesses of external and internal relations of the logistics. From this perspective it was observed that the implementation of the SCOR model makes more efficient the logistics operations through the whole chain.

  14. Tratamiento de las fracturas diafisarias de húmero mediante osteosíntesis con placa

    OpenAIRE

    Zamora Rodríguez, J. M.; Modrego Aranda, Francisco Javier; Seral García, Belén; Seral Iñigo, Fernando

    2002-01-01

    Hemos tratado 22 facturas agudas diafisarias de húmero mediante reducción abierta y fijación con placa AO entre 1991 y 1999. Todas las fracturas excepto una consolidaron en un plazo medio de 94 días. De acuerdo con el criterio de Brumback los resultados fueron excelentes o buenos en el 90% de los casos. La complicación postoperativa más importante fue la parálisis del nervio radial en 3 casos; todos se recuperaron espontáneamente en un plazo medio de 108 días. La fijación interna mediante pla...

  15. Modulación del crecimiento vertebral mediante electrocoagulación hemicircunferencial vertebral asistida

    OpenAIRE

    Caballero García, Alberto

    2009-01-01

    Nuestro trabajo está basado en la posibilidad de controlar el desarrollo asimétrico de los cartílagos de crecimiento vertebral, mediante la realización de una fisiodesis hemivertebral, con electrocoagulación, videoasistida por toracoscópica. Se realizará en cinco niveles torácicos, con un abordaje anterior mínimamente invasivo. Por lo tanto, planteamos como hipótesis de trabajo que La destrucción de las fisis de crecimiento vertebral mediante electrocoagulación, videoasistida por vía toracosc...

  16. Reconocimiento de textos antiguos mediante técnicas de visión artficial

    OpenAIRE

    Rodríguez Vallejo, Moisés

    2013-01-01

    El objetivo de este proyecto es reconocer caracteres de textos antiguos mediante t ecnicas de visi on arti cial y redes neuronales. Distinguimos dos fases en el proceso: extracci on de los caracteres de las im agenes de los textos antiguos; y el reconocimiento mediante redes neuronales. En la primera utilizaremos las librer as de OpenCV para conseguir el objetivo, mientras que en la segunda fase partimos de un c odigo de una red neuronal de 3 capas de la Universidad Carnegie M...

  17. Simulación y llenado de moldes mediante Moldflow®

    OpenAIRE

    Peidró Roy, Alejandro

    2015-01-01

    El objetivo del presente proyecto es recopilar los conocimientos necesarios para que los alumnos de Formación Professional del ciclo formativo de Diseño mecánico en el módulo M5 Moldes poliméricos, partiendo de cero, puedan diseñar un pieza de plástico y su molde de inyección asociado mediante el software Catia® en los módulos de Part desing, Generative Shape desing y Mold Tooling desing, simularla mediante Moldflow® Plastics, rediseñarla y optimizarla, de forma que el documento que se redact...

  18. Development of antigen capture ELISA for the quantification of EIAV p26 protein.

    Science.gov (United States)

    Hu, Zhe; Chang, Hao; Ge, Man; Lin, Yuezhi; Wang, Xuefeng; Guo, Wei; Wang, Xiaojun

    2014-11-01

    An antigen capture enzyme-linked immunosorbent assay (AC-ELISA) was established based on two monoclonal antibodies (mAbs) for the quantification of equine infectious anemia virus (EIAV). Two p26-specific monoclonal antibodies were developed in mice. The mAb 9H8 was coated in microtiter plates as the capture antibody; the other mAb, 1G11, was coupled to horseradish peroxidase (HRP) and used as the detection antibody. The limit of detection for the EIAV p26 protein was 0.98 ng/ml, and the linearity range was 3.9-62.5 ng/ml. The sensitivity of p26 AC-ELISA for the detection of the virus (EIAV infectious clone, FDDVcmv3-8) was the same as that for the purified p26 protein. No cross-reaction with other equine viruses was observed by this method. The intra- and inter-assay coefficients of variation were below 8.3 and 10.3 % for testing p26 and FDDVcmv3-8, respectively. The AC-ELISA was also compared to Western blotting (WB) and reverse transcriptase (RT) assays, validating the sensitivity, accuracy, and reliability of this method. Both the AC-ELISA and RT assay showed good agreement, with a correlation coefficient of R (2) =0.9946. Sample analysis showed that this AC-ELISA is a useful tool for quantifying EIAV p26 in cell lysates and culture medium.

  19. Nanospherical Brush as Catalase Container for Enhancing the Detection Sensitivity of Competitive Plasmonic ELISA.

    Science.gov (United States)

    Huang, Xiaolin; Chen, Rui; Xu, Hengyi; Lai, Weihua; Xiong, Yonghua

    2016-02-01

    Plasmonic enzyme-linked immunosorbent assay (pELISA) based on catalase (CAT)-mediated gold nanoparticle growth shows great potential for the determination of disease-related biomarkers at ultralow concentrations by using sandwich formats. However, the relatively low sensitivity of this strategy using competitive formats limits its adoption for hapten detection. Herein, we present an improved competitive pELISA for ultrasensitive detection of ochratoxin A (OTA), where silica nanoparticles carrying poly(acrylic acid) brushes (SiO2@PAA) were used to decrease the affinity of competing antigens to anti-OTA monoclonal antibodies and amplify the signal as a "CAT container" (SiO2@PAA@CAT). The developed competitive pELISA exhibits extremely high sensitivity for OTA with detection limits of 10(-18) and 5 × 10(-20) g/mL by the naked eye and microplate reader, respectively. These values are at least 7 orders of magnitude lower than that of competitive CAT-based pELISA (10(-11) g/mL by the naked eye) and 8 orders of magnitude lower than that of horseradish peroxidase-based conventional ELISA (10(-11) g/mL by the microplate reader), respectively. Reliability and robustness of the proposed method were evaluated using actual agricultural products and human serum samples. This study demonstrated the potential of this modified method in practical applications involving the ultrasensitive detection of mycotoxins or other haptens.

  20. An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus

    Directory of Open Access Journals (Sweden)

    Åkerstedt J

    2002-12-01

    Full Text Available Infection with the intracellular microsporidium Encephalitozoon cuniculi can cause serious disease, encephalitozoonosis, in the blue fox (Alopex lagopus. The disease diagnosis is based on clinical signs and pathological findings, and detection of E. cuniculi or circulating antibodies directed against the parasite. Indirect immunofluorescence (IFAT and carbon immunoassay (CIA are the most commonly used serological methods for diagnosis in this species. In the present study, an indirect ELISA (enzyme linked immunosorbent assay was established and evaluated against IFAT by testing of 205 field samples from blue foxes. There was high agreement between the results of the ELISA and CIA (κ = 0.99, and the ELISA and IFAT (κ = 0.958. There was no significant statistical difference between the tests (p > 0.05. It was concluded that the ELISA could be used to identify seropositive farmed blue foxes. The advantage of the ELISA lies in the potential of screening large numbers of animals with the goal of eradicating E. cuniculi infection in the farms.

  1. Development of an Indirect ELISA for Serological Diagnosis of Bovine herpesvirus 5.

    Directory of Open Access Journals (Sweden)

    Luana A Dummer

    Full Text Available Bovine herpesviruses 1 and 5 (BoHV-1 and BoHV-5 are economically important pathogens, associated with a variety of clinical syndromes, including respiratory and genital disease, reproductive failure and meningoencephalitis. The standard serological assay to diagnose BoHV-1 and BoHV-5 infections is the virus neutralization test (VNT, a time consuming procedure that requires manipulation of infectious virus. In the present study a highly sensitive and specific single dilution indirect ELISA was developed using recombinant glycoprotein D from BoHV-5 as antigen (rgD5ELISA. Bovine serum samples (n = 450 were screened by VNT against BoHV-5a and by rgD5ELISA. Compared with the VNT, the rgD5ELISA demonstrated accuracy of 99.8%, with 100% sensitivity, 96.7% specificity and coefficient of agreement between the tests of 0.954. The rgD5ELISA described here shows excellent agreement with the VNT and is shown to be a simple, convenient, specific and highly sensitive virus-free assay for detection of serum antibodies to BoHV-5.

  2. A Sensitive Competitive ELISA for Determination of Biotin in Transformed Yeast Culture Media

    Institute of Scientific and Technical Information of China (English)

    YANGHong

    2003-01-01

    Aim To develop a sensitive competitive ELISA for the determination of biotin in transformed yeast culture media.Methods The ELISA plate was firstly coated with Mycoplasma hyopneumoniae, and then successively incubated with rabbit ami-Mycoplasma hyopneumoniae serum and goat anti-rabbit IgG-biotin to form the solid biotin, which competed with the biotin in the solution (standard or sample) for the limited streptavidin-horse radish peroxidase conjugate. The standard calibration curve for biotin analysis was constructed in the range of 50-2000ng·L-1. Results The detection limit for biotin was found to be 83 ng·L-1 , which waa about 1000 times lower than the lowest determination concenlration in the reported ELISA for biotin analysis. The relative standard deviations for the spiked samples at biotin concerarations of 200 ng·L-1, 500 ng·L-1 , and 1000 ng·L-1 were 24.87%, 6.15%, and 7.86%, respectively, with the average recovery of 101.13%. The wild yeast and its sixty-three transformed yeast culture media were applied to the developed ELISA for the determination of biotin. It was found that the biotin concentrations in more than 85 % of the tested samples were enhanced with different increase factors after transformation. Conclusion Utilization of Mycoplasma hyopnetunoniae as the coating protein improves the precision and accmacy oftbe ELISA assay, which might be used for the biotin assay in other media.

  3. Development of an Indirect ELISA for Serological Diagnosis of Bovine herpesvirus 5

    Science.gov (United States)

    Campos, Fabrício S.; da Rosa, Matheus C.; Finger, Paula F.; de Oliveira, Patricia D.; Conceição, Fabricio R.; Fischer, Geferson; Roehe, Paulo M.; Leite, Fábio P. L.

    2016-01-01

    Bovine herpesviruses 1 and 5 (BoHV-1 and BoHV-5) are economically important pathogens, associated with a variety of clinical syndromes, including respiratory and genital disease, reproductive failure and meningoencephalitis. The standard serological assay to diagnose BoHV-1 and BoHV-5 infections is the virus neutralization test (VNT), a time consuming procedure that requires manipulation of infectious virus. In the present study a highly sensitive and specific single dilution indirect ELISA was developed using recombinant glycoprotein D from BoHV-5 as antigen (rgD5ELISA). Bovine serum samples (n = 450) were screened by VNT against BoHV-5a and by rgD5ELISA. Compared with the VNT, the rgD5ELISA demonstrated accuracy of 99.8%, with 100% sensitivity, 96.7% specificity and coefficient of agreement between the tests of 0.954. The rgD5ELISA described here shows excellent agreement with the VNT and is shown to be a simple, convenient, specific and highly sensitive virus-free assay for detection of serum antibodies to BoHV-5. PMID:26866923

  4. The Measurement of C- Reactive Protein (CRP using Magnetic Nanobeads Add ELISA

    Directory of Open Access Journals (Sweden)

    Baharifar, H. (MSc

    2014-05-01

    Full Text Available Background and Objective: Magnetic nanobeads have a large surface- area-to-volume ratio, which is used for immobilized antibody. Using nanoparticles could increase the amount of antibodies in surface in comparison to ELISA. We investigated the ability of magnetic nanobeads to evaluate CRP by colorimetric method and compared the results with ELISA. Material and Methods: This study is an applicable research conducted in Tehran University of Medical Sciences, 2012. The Magnetic nanobeads conjugated by CRP antibodies were used to measure the protein in the concentration of 1-10 ng/ml (ELISA kit levels and 0.1 – 0.01 ng/ml. After antigen measurement, the results were compared with Mann Whitney test. Results: The results in concentration of 1-10 ng/ml are not significantly different (p = 0.78. But in concentrations of 0.1-0.01 ng/ml, the difference is significant (p= 0.02. Conclusion: The ability of Magnetic nanobeads in measurement of low concentration of antigen is 100 times better than ELISA. Thus Magnetic nanobead method is useful for early measurement and can easily be used in clinical laboratory. Keywords: CRP; Magnetic Nanobead; ELISA

  5. Screening of plants containing Naja naja siamensis cobra venom inhibitory activity using modified ELISA technique.

    Science.gov (United States)

    Daduang, Sakda; Sattayasai, Nison; Sattayasai, Jintana; Tophrom, Pattara; Thammathaworn, Achra; Chaveerach, Arunrat; Konkchaiyaphum, Monruedee

    2005-06-15

    Enzyme-linked immunosorbent assay (ELISA) has been modified for screening plants with antagonistic activity to Naja naja siamensis cobra venom. Aqueous extracts from plants were investigated for their inhibitory effects on the binding of anti-cobra venom antibody to antigen, cobra venom, fixed onto 96-well microtiter plates. Ingredients in extracts were allowed to react with immobilized venom before the subsequent addition of antivenom antibody. Venom components affected by exposure to the extracts, unable to interact with their specific antibody, were predicted to be unable to bind to their native destinations or natural receptors. Curcuma cf. zedoaria, an old Thai medicinal plant, showed clear inhibitory activity in the ELISA test. Neurotoxin and protein degradative enzymes, major components in venom, were identified as targets of this extract in Western immunoblotting analysis. Ingredients in the extract showed high affinity to the toxin in competition assay by immunoprecipitation. The extract attenuated toxin activity by extending contraction time of diaphragm muscle after envenomation and had a potency to protect cellular proteins from venom degradative enzymes. Curcuma parviflora, with less activity in ELISA, exhibited acceptable results in two experiments but negative results in two experiments, whereas Curcuma longa, having low activity in the ELISA test, never showed any favorable results. Screening of 36 samples could classify plants into an inhibition range of 0 to 86%. This modified ELISA is recommended as a preliminary screening method for inhibitors with a large number of samples.

  6. Development of a species-specific coproantigen ELISA for human Taenia solium taeniasis.

    Science.gov (United States)

    Guezala, Maria-Claudia; Rodriguez, Silvia; Zamora, Humberto; Garcia, Hector H; Gonzalez, Armando E; Tembo, Alice; Allan, James C; Craig, Philip S

    2009-09-01

    Taenia solium causes human neurocysticercosis and is endemic in underdeveloped countries where backyard pig keeping is common. Microscopic fecal diagnostic methods for human T. solium taeniasis are not very sensitive, and Taenia saginata and Taenia solium eggs are indistinguishable under the light microscope. Coproantigen (CoAg) ELISA methods are very sensitive, but currently only genus (Taenia) specific. This paper describes the development of a highly species-specific coproantigen ELISA test to detect T. solium intestinal taeniasis. Sensitivity was maintained using a capture antibody of rabbit IgG against T. solium adult whole worm somatic extract, whereas species specificity was achieved by utilization of an enzyme-conjugated rabbit IgG against T. solium adult excretory-secretory (ES) antigen. A known panel of positive and negative human fecal samples was tested with this hybrid sandwich ELISA. The ELISA test gave 100% specificity and 96.4% sensitivity for T. solium tapeworm carriers (N = 28), with a J index of 0.96. This simple ELISA incorporating anti-adult somatic and anti-adult ES antibodies provides the first potentially species-specific coproantigen test for human T. solium taeniasis.

  7. His-tag ELISA for the detection of humoral tumor-specific immunity

    Directory of Open Access Journals (Sweden)

    Disis Mary L

    2008-05-01

    Full Text Available Abstract Background The application of high throughput molecular techniques such as SEREX are resulting in the identification of a multitude of tumor associated antigens. As newly identified antigens are incorporated into a variety of clinical trials, standardization of immunologic monitoring methods becomes increasingly important. We questioned whether mammalian cell expression of a histadine-linked human protein could be used to produce antigen suitable for detecting tumor-specific humoral immunity and whether such an assay could be amenable to standardization for clinical use. Methods We designed a his-tagged capture ELISA based on lysate from genetically engineered CHO cells for detection of antibodies to insulin-like growth factor binding protein 2, a novel tumor antigen. We performed technical and preliminary clinical validation studies, including comparison to a standard indirect ELISA based on commercially prepared recombinant antigen. Results The his-tagged capture ELISA could be standardized. Precision experiments resulted in CVs 2 values of 0.99. In comparison to Western blot analysis, his-tag and indirect ELISA accurately identified 88% and 93% of samples, respectively. Sample concordance between capture and indirect assays was highly significant (p = 0.003. Furthermore, significantly greater levels of IGFBP-2 antibody immunity were found in cancer patients compared to normal controls (p = 0.008. Conclusion A genetically engineered cell lysate based ELISA can be amenable to standardization and can detect increased levels of antibody immunity to tumor-associated antigen in cancer patients compared to non tumor-bearing healthy controls.

  8. Development of an antibody capture ELISA using inactivated Ebola Zaire Makona virus.

    Science.gov (United States)

    Krähling, Verena; Becker, Dirk; Rohde, Cornelius; Eickmann, Markus; Eroğlu, Yonca; Herwig, Astrid; Kerber, Romy; Kowalski, Katharina; Vergara-Alert, Júlia; Becker, Stephan

    2016-04-01

    The 2014 Zaire Ebola virus (ZEBOV) outbreak in West Africa represents an international public health concern. Highly sensitive and precise diagnostic tools are needed. In the present study, we developed a ZEBOV-specific enzyme-linked immunosorbent assay (ELISA) using inactivated ZEBOV isolate Makona from March 2014. Mock antigen was used to address nonspecific binding. Specificity, reproducibility and precision were determined to measure assay performance. The ZEBOV ELISA proved to be specific (96 %), reproducible and precise (Intra-assay CV 8 %, Inter-assay CV 18 %). Using the human monoclonal antibody KZ52, we showed that the ELISA was able to detect conformation-specific antibodies. Monitoring antibody development in 29 PCR-positive EBOV disease (EVD) patients revealed seroconversion in all cases. In addition, the ELISA was used to detect ZEBOV glycoprotein (GP)-specific antibodies in a vaccinated volunteer from day 14 until 5 years post-vaccination with a VSV-ZEBOV candidate vaccine. The results demonstrate the high reproducibility, specificity and sensitivity of this newly developed ELISA, which is suitable for the detection of specific antibody responses directed against different ZEBOV proteins in EVD patients and against the ZEBOV surface glycoprotein GP in vaccinated individuals.

  9. Validation of ELISA for the detection of African horse sickness virus antigens and antibodies.

    Science.gov (United States)

    Rubio, C; Cubillo, M A; Hooghuis, H; Sanchez-Vizcaino, J M; Diaz-Laviada, M; Plateau, E; Zientara, S; Crucière, C; Hamblin, C

    1998-01-01

    The mortality rate in susceptible populations of horses during an epizootic of African horse sickness (AHS) may be in excess of 90%. Rapid and reliable assays are therefore essential for the confirmation of clinical diagnoses and to enable control strategies to be implemented without undue delay. One of the major objectives of a recent European Union funded project was the validation of newly developed diagnostic assays which are rapid, sensitive, highly reproducible and inexpensive, for the detection of African horse sickness virus (AHSV) antigens and antibodies. The Laboratorio de Sanidad y Produccion Animal (LSPA) in Algete, Spain was charged with the responsibility of co-ordinating and supplying samples of viruses and antisera to the participating laboratories in Spain, France and the United Kingdom. The panels comprised 76 antigen samples for assay by indirect sandwich ELISAs and 53 serum samples for antibody detection by either indirect or competitive ELISAs. Results generated by ELISA for each laboratory were analysed in LSPA in terms of their relative sensitivities and specificities. There was a good agreement between the ELISAs used for either antigen or antibody detection. The participating groups agreed that any field sample giving a doubtful result would always be retested by ELISA and an alternative assay.

  10. Development of indirect sandwich ELISA for determination of excretory-secretory antigens of Fasciola hepatica

    Directory of Open Access Journals (Sweden)

    Libertad Alzamora-Gonzales

    2016-05-01

    Full Text Available Fasciolosis is a cosmopolitan parasitosis medical-veterinary importance caused by Fasciola hepatica, which affects sheep, goats and cattle; and it affects man accidentally causing an epidemic-endemic infection difficult to diagnose. The aim was to develop an indirect sandwich ELISA with 3 antibodies for detecting excretory-secretory antigens of Fasciola hepatica (ESFh. For the development of indirect sandwich ELISA were used, as capture antibody, mouse polyclonal antibodies anti ESFh and polyclonal antibodies rabbit anti-ESFh as detection antibody, at the concentrations of 10 and 5 µg/mL respectively. The conjugate used was mouse monoclonal anti- total immunoglobulins rabbit linked to peroxidase (1/1000. Were analized 31 sheep fecal samples, and the results were compared with those obtained by direct coproparasitological examination (DC and counterimmunoelectrophoresis (CIEP. The detection limit obtained for indirect sandwich ELISA was 100 ng/mL. The test had a 100% sensitivity, 96.6% specificity, positive and negative predictive values of 50% and 96.6% respectively, in relation to DC test. Comparing with CIEP the specificity obtained for indirect sandwich ELISA was 93.5% and a negative predictive value of 100%. We concluded that indirect sandwich ELISA designed is able to detect metabolic antigens in ovine feces samples and can be used for Fasciola hepatica diagnosis.

  11. Comparison studies on testing GFLV and with DAS-ELISA and PAS-ELISA%DAS-ELISA和PAS-ELISA检测GFLV的技术研究

    Institute of Scientific and Technical Information of China (English)

    陈建军; 李培睿; 杨向英; 曹香林; 刘崇怀

    2004-01-01

    以中国农科院郑州果树研究所国家葡萄资源圃的葡萄品种为试材,对葡萄扇叶病毒(GFLV)进行了酶联免疫吸附测定(ELISA)的检测技术研究.从操作步骤、灵敏度、检测时期以及检测部位等对双抗体夹心酶联免疫吸附测定法(DAS-ELISA)和A蛋白夹心酶联免疫吸附测定法(PAS-ELISA)2种不同的检测方法进行了比较试验.发现,虽然DAS-ELISA在操作上较PAS-ELISA更为简便,但就结果而言,PAS-ELISA比DAS-ELISA的OD值高,更易进行结果的判断;且在病毒含量相对较低的情况下,PAS-ELISA显示出了较高的灵敏度.

  12. Low-frequency Gravitational-wave Science with eLISA

    Science.gov (United States)

    Vallisneri, Michele; eLISA Science Study Team

    2013-01-01

    I review the expected science performance of eLISA, the proposed European-led gravitational-wave detector that may be selected as ESA "L2" Cosmic Vision mission. eLISA will survey the low-frequency gravitational-wave sky (from 0.1 mHz to 1 Hz), detecting and characterizing a broad variety of systems and events throughout the Universe, including the coalescences of massive black holes brought together by galaxy mergers; the inspirals of stellar-mass black holes and compact stars into central galactic black holes; several millions of ultracompact binaries, both detached and mass transferring, in the Galaxy; and possibly unforeseen sources such as the relic gravitational-wave radiation from the early Universe. eLISA's high signal-to-noise measurements will provide new insight into the structure and history of the Universe, and they will test general relativity in its strong-field dynamical regime.

  13. Development of sandwich ELISAs for the detection of aromatic diisocyanate adducts.

    Science.gov (United States)

    Lemons, Angela R; Bledsoe, Toni A; Siegel, Paul D; Beezhold, Donald H; Green, Brett J

    2013-11-29

    Diisocyanates (dNCOs) are highly reactive low molecular weight chemicals commonly used in the manufacturing industry. Occupational exposures to dNCOs have been shown to elicit allergic sensitization and occupational asthma. Among the most commonly used dNCOs in industry are the aromatic dNCOs, toluene diisocyanate (TDI) and methylene diphenyl diisocyanate (MDI). This study aimed to develop enzyme linked immunosorbent assays (ELISA) utilizing aromatic dNCO-specific monoclonal antibodies (mAbs) for the detection of aromatic dNCO adducts. Two sandwich ELISAs were developed. The first sandwich ELISA utilized mAb 60G2 along with an anti-human serum albumin (HSA) polyclonal antibody. This assay detected MDI-, 2,4- and 2,6-TDI-HSA adducts with limits of detection (LOD) of 2.67, characterization of aromatic dNCO adducts as well as in biomonitoring occupational and environmental dNCO exposures. PMID:24012971

  14. Immunoreactive insulin in diabetes mellitus patient sera detected by ultrasensitive ELISA with thio-NAD cycling.

    Science.gov (United States)

    Ito, Etsuro; Kaneda, Mugiho; Kodama, Hiromi; Morikawa, Mika; Tai, Momoko; Aoki, Kana; Watabe, Satoshi; Nakaishi, Kazunari; Hashida, Seiichi; Tada, Satoshi; Kuroda, Noriyuki; Imachi, Hitomi; Murao, Koji; Yamashita, Masakane; Yoshimura, Teruki; Miura, Toshiaki

    2015-12-01

    To minimize patient suffering, the smallest possible volume of blood should be collected for diagnosis and disease monitoring. When estimating insulin secretion capacity and resistance to insulin in diabetes mellitus (DM), increasing insulin assay immunosensitivity would reduce the blood sample volume required for testing. Here we present an ultrasensitive ELISA coupled with thio-NAD cycling to measure immunoreactive insulin in blood serum. Only 5 μL of serum was required for testing, with a limit of detection (LOD) for the assay of 10(-16) moles/assay. Additional recovery tests confirmed this method can detect insulin in sera. Comparisons between a commercially available immunoreactive insulin kit and our ultrasensitive ELISA using the same commercially available reference demonstrated good data correlation, providing further evidence of assay accuracy. Together, these results demonstrate our ultrasensitive ELISA could be a powerful tool in the diagnosis and treatment of not only DM but also many other diseases in the future.

  15. IgG Adhesion on Hydrophobic Surfaces: Theory, Modelling, and Application to ELISA

    CERN Document Server

    de Thier, P

    2016-01-01

    Enzyme-Linked ImmunoSorbent Assays (ELISA) are a range of widely used analytical methods whose implementation requires to build antibodies (IgG) thin films onto surfaces predominantly made of polystyrene. The high hydrophobicity of polystyrene ensures a spontaneous and strong adhesion of proteins allowing to easily build IgG monolayers. Since the ELISA improvements definitely lie in the comprehension of physico-chemical mechanisms on which IgG immobilization on hydrophobic surfaces are relied, this work develops a theorization essay (thermodynamics of the so-called hydrophobic effect and of thin films building) emphasized by numerical modelling (random sequential additions model, i.e. RSA) and experimental estimations by atomic force microscopy (AFM) and ELISA. Keeping in mind the hydrophobic effect, thermodynamics (of irreversible processes) allows to explain why IgG adhesion on polystyrene occurs whereas numerical modelling approaches show a way of surface saturation leading to promote IgG orientations expe...

  16. Prevalence of Bovine Brucellosis in Organized Dairy Farms, Using Milk ELISA, in Quetta City, Balochistan, Pakistan

    Directory of Open Access Journals (Sweden)

    Muhammad Shafee

    2011-01-01

    Full Text Available A total of 200 milk samples from cattle (=86 and buffalo (=114 were evaluated using milk ring test (MRT and indirect enzyme-linked immunosorbent assay (i-ELISA. The overall prevalence was found to be 3% and 8.5% in cattle and buffaloes using MRT and i-ELISA, respectively. The prevalence was 4.6% and 1.7% in cattle and buffalo using MRT, respectively, while i-ELISA exhibited 20% and 0% in cattle and buffalo, respectively. The prevalence was higher in government dairy farm, compared to privately owned dairy farm. This paper points out an alarming situation in the target area with respect to the public health significance.

  17. Prevalence of bovine brucellosis in organized dairy farms, using milk ELISA, in quetta city, balochistan, pakistan.

    Science.gov (United States)

    Shafee, Muhammad; Rabbani, Masood; Sheikh, Ali Ahmad; Ahmad, Mansoor Din; Razzaq, Abdul

    2011-01-24

    A total of 200 milk samples from cattle (n = 86) and buffalo (n = 114) were evaluated using milk ring test (MRT) and indirect enzyme-linked immunosorbent assay (i-ELISA). The overall prevalence was found to be 3% and 8.5% in cattle and buffaloes using MRT and i-ELISA, respectively. The prevalence was 4.6% and 1.7% in cattle and buffalo using MRT, respectively, while i-ELISA exhibited 20% and 0% in cattle and buffalo, respectively. The prevalence was higher in government dairy farm, compared to privately owned dairy farm. This paper points out an alarming situation in the target area with respect to the public health significance.

  18. Investigation of Entamoeba histolytica in stool specimens by ELISA during a year

    Directory of Open Access Journals (Sweden)

    Mehmet Sinan Dal

    2011-03-01

    Full Text Available The aim of this study was to investigate Entamoebahistolytica in the stool samples that have beensent to microbiology laboratory by ELISA method.Materials and methods: This study was performed on800 stool specimens belonging to different age groupsand different patients sent to Microbiology Laboratory ofa State Hospital between January 2009 and December2009.Results: In this study Entamoeba histolytica/dispar cystsand/or trophozoites were observed in 31 (3.9% out of atotal of 800 stool specimens which were examined bynative-lugol. Entamoeba histolytica specific antigen wasinvestigated by ELISA in all stool specimens. Entamoebahistolytica specific antigen was detected in 12 (1.5% ofthese stool specimens. The diagnosis of amoebiasis forthe patients whose ELISA tests were “positive” and appropriatetherapy was begun.Conclusion: Entamoeba histolytica in the stool samplesshould be investigated and unnecessary treatmentsshould not given to patients. J Clin Exp Invest 2011; 2(1:50-54

  19. Follow-up of neurocysticercosis patients after treatment using an antigen detection ELISA

    Directory of Open Access Journals (Sweden)

    Nguekam

    2003-03-01

    Full Text Available Seven patients with active neurocysticercosis (NCC received an eight days treatment with albendazole and were followed up using computed tomography (CT-scan and a monoclonal antibody based ELISA for the detection of circulating antigen (Ag-ELISA. Only three patients were cured as was shown by CT-scan and by the disappearance of circulating antigens one month after treatment. After a second course of albendazole therapy, two other patients became seronegative. CT-scan showed the disappearance of viable cysts in all persons who became seronegative whereas patients who were not cured remained seropositive. These preliminary results show that this Ag-ELISA is a promising technique for monitoring the success of treatment of NCC patients because of the excellent correlation between the presence of circulating antigens and of viable brain cysts.

  20. Field trial of a brucellosis competitive enzyme linked immunoabsorbent assay (ELISA)

    International Nuclear Information System (INIS)

    The purpose of this study was to evaluate the performance of a competitive ELISA system for the diagnosis of bovine brucellosis in comparison to conventional aerological tests routinely used in Argentina. A total of 2.500 serum samples, comprising Brucella-free herds, vaccinated cattle and naturally infected animals, was tested by the following tests: buffered plate agglutination, Rose Bengal, 2-mercaptoethanol, complement fixation, and indirect and competitive ELISAs. Specificity and relative sensitivity at each test were determined. The competitive ELISA was considered suitable for detection of vaccinated animals and had higher specificity than the other tests. The results point to the potential use of the test as a complementary assay in the brucellosis control programme in Argentina. (author)

  1. Development and use of ELISA in the control of foot-and-mouth disease

    International Nuclear Information System (INIS)

    Foot-and-mouth disease (FMD) is a highly contagious and economically devastating disease that requires laboratory investigation for definitive diagnosis. Highly specific and sensitive assays are needed to obtain a rapid result and so optimize the efficiency of FMD diagnosis and formulation of appropriate control measures. The development of the enzyme linked immunosorbent assay (ELISA) has produced marked improvements in the diagnosis of FMD. An indirect sandwich ELISA has replaced the complement fixation test for FMDV virus antigen detection and a liquid phase blocking ELISA (LPBE) has replaced the virus neutralization test for the detection of serum antibodies. The LPBE is also employed to test the suitability of available vaccine strains for use in current outbreaks. The assays mainly use polyclonal antisera, but monoclonal antibodies have brought greater precision to virus strain characterization studies and have the potential to replace polyclonal sera in other assays. The provision of ELISA kits enables national FMD laboratories in developing countries to carry out their own assays in the first instance and allows effective and more immediate decisions to be made on control. At present, there are no reliable mass serum screen laboratory tests (i.e. ELISA) that can distinguish vaccinated from infected and carrier from non-carrier animals. These groups of animals have to be considered potential carriers of FMD virus and a source of new disease and thus need to be excluded from internal and international trade. ELISAs employing reagents to bioengineered non-structural proteins of FMD virus hold considerable promise for the discrimination of naive, convalescent and vaccinated animals, and work is in progress for their refinement. (author)

  2. Dispensing an enzyme-conjugated solution into an ELISA plate by adapting ink-jet printers.

    Science.gov (United States)

    Lonini, Luca; Accoto, Dino; Petroni, Silvia; Guglielmelli, Eugenio

    2008-04-24

    The rapid and precise delivery of small volumes of bio-fluids (from picoliters to nanoliters) is a key feature of modern bioanalytical assays. Commercial ink-jet printers are low-cost systems which enable the dispensing of tiny droplets at a rate which may exceed 10(4) Hz per nozzle. Currently, the main ejection technologies are piezoelectric and bubble-jet. We adapted two commercial printers, respectively a piezoelectric and a bubble-jet one, for the deposition of immunoglobulins into an ELISA plate. The objective was to perform a comparative evaluation of the two classes of ink-jet technologies in terms of required hardware modifications and possible damage on the dispensed molecules. The hardware of the two printers was modified to dispense an enzyme conjugate solution, containing polyclonal rabbit anti-human IgG labelled with HRP in 7 wells of an ELISA plate. Moreover, the ELISA assay was used to assess the functional activity of the biomolecules after ejection. ELISA is a common and well-assessed technique to detect the presence of particular antigens or antibodies in a sample. We employed an ELISA diagnostic kit for the qualitative screening of anti-ENA antibodies to verify the ability of the dispensed immunoglobulins to bind the primary antibodies in the wells. Experimental tests showed that the dispensing of immunoglobulins using the piezoelectric printer does not cause any detectable difference on the outcome of the ELISA test if compared to manual dispensing using micropipettes. On the contrary, the thermal printhead was not able to reliably dispense the bio-fluid, which may mean that a surfactant is required to modify the wetting properties of the liquid.

  3. Evaluation of dengue NS1 antigen rapid tests and ELISA kits using clinical samples.

    Directory of Open Access Journals (Sweden)

    Subhamoy Pal

    Full Text Available Early diagnosis of dengue virus (DENV infection can improve clinical outcomes by ensuring close follow-up, initiating appropriate supportive therapies and raising awareness to the potential of hemorrhage or shock. Non-structural glycoprotein-1 (NS1 has proven to be a useful biomarker for early diagnosis of dengue. A number of rapid diagnostic tests (RDTs and enzyme-linked immunosorbent assays (ELISAs targeting NS1 antigen (Ag are now commercially available. Here we evaluated these tests using a well-characterized panel of clinical samples to determine their effectiveness for early diagnosis.Retrospective samples from South America were used to evaluate the following tests: (i "Dengue NS1 Ag STRIP" and (ii "Platelia Dengue NS1 Ag ELISA" (Bio-Rad, France, (iii "Dengue NS1 Detect Rapid Test (1st Generation" and (iv "DENV Detect NS1 ELISA" (InBios International, United States, (v "Panbio Dengue Early Rapid (1st generation" (vi "Panbio Dengue Early ELISA (2nd generation" and (vii "SD Bioline Dengue NS1 Ag Rapid Test" (Alere, United States. Overall, the sensitivity of the RDTs ranged from 71.9%-79.1% while the sensitivity of the ELISAs varied between 85.6-95.9%, using virus isolation as the reference method. Most tests had lower sensitivity for DENV-4 relative to the other three serotypes, were less sensitive in detecting secondary infections, and appeared to be most sensitive on Day 3-4 post symptom onset. The specificity of all evaluated tests ranged from 95%-100%.ELISAs had greater overall sensitivity than RDTs. In conjunction with other parameters, the performance data can help determine which dengue diagnostics should be used during the first few days of illness, when the patients are most likely to present to a clinic seeking care.

  4. Procesamiento de muestras fecales en el estudio de Cryptosporidium sp. mediante PCR

    Directory of Open Access Journals (Sweden)

    Gregorio Pérez-Cordón

    2013-05-01

    Full Text Available El presente trabajo describe un protocolo aplicado a muestras de heces de ganado bovino para el aislamiento y purificación de ooquistes de Cryptosporidium sp. y el eventual uso de los mismos para estudios genéticos mediante PCR.

  5. Development and validation of a competitive ELISA kit for the serological diagnosis of ovine, caprine and bovine brucellosis.

    Science.gov (United States)

    Portanti, O; Tittarelli, M; Di Febo, T; Luciani, M; Mercante, M T; Conte, A; Lelli, R

    2006-12-01

    A competitive ELISA (Brucella-Ab c-ELISA) was standardized and validated for the detection of Brucella antibodies in cattle, sheep and goat sera using a monoclonal antibody (MAb 4B5A) produced against Brucella melitensis biotype 2. The specificity and sensitivity of the assay were 100% to a 67.5% cut-off point (B/Bo%). When compared with an indirect ELISA, the Brucella-Ab c-ELISA did not demonstrate cross-reactions when testing positive sera for antibodies to some Enterobacteriaceae. A comparison was made between the Brucella-Ab c-ELISA and the complement fixation and Rose Bengal tests. Results demonstrated that the Brucella-Ab c-ELISA is a valuable tool for the serological diagnosis of bovine and ovine/caprine brucellosis. PMID:17123429

  6. Analysis of Detecting HIV-1 Antibody in Paired Urine and Serum Specimens from Drug Users by ELISA

    Institute of Scientific and Technical Information of China (English)

    刘中夫; 李志军; 刘世亮; 李莉; 梁富雄; 郑锡文

    2001-01-01

    Objective: To compare the consistency of the results from detecting HIV-1 antibody in the paired urine and serum specimens from drug users by ELISA.Methods: The paired urine and serum specimens from 273 drug users detained at a detoxification unit were collected, and the HIV-1 antibodies in the specimens of them were screened by urine and serum ELISA kits, respectively. Results: Of 273 serum specimens, 94 ones showed positive reaction and among 94 counterpart urine specimens, 93 ones also appeared positive reaction. Taking the results together,the consistent rate of HIV-1 antibody screened by urine and serum ELISA kits was 99.6%.Conclusion: The urine ELISA kit, which screened HIV-1 antibody of urine showing almost the same results tested by serum ELISA kit, is reliable. It is proposed that urine ELISA be introduced in many fields.

  7. Evaluation of a blocking ELISA for screening of antibodies against porcine reproductive and respiratory syndrome (PRRS) virus

    DEFF Research Database (Denmark)

    Sørensen, K.J.; Bøtner, Anette; Madsen, E.S.;

    1997-01-01

    A blocking Elisa was developed for the detection of antibodies against PRRS virus with a view to satisfying the need for examination of blood samples on a large scale. The test was evaluated in comparison with an indirect Elisa and the immunoperoxidase monolayer assay. The blocking Elisa...... was sensitive and specific. It had a higher capacity and was cheaper to perform than the immunoperoxidase monolayer assay and the indirect Elisa. It was comparable to the immunoperoxidase monolayer assay and better than the indirect Elisa in detecting antibodies formed early after infection, and it was superior...... to both the immunoperoxidase monolayer assay and the indirect Elisa in detecting antibodies at a late stage of infection....

  8. Bayesian estimation of sensitivity and specificity of Coxiella burnetii antibody ELISA tests in bovine blood and milk

    DEFF Research Database (Denmark)

    Paul, Suman; Toft, Nils; Agerholm, Jørgen S.;

    2013-01-01

    Serological tests for Coxiella burnetii (the causative agent of Q fever) antibodies are usually based on enzyme linked immunosorbent assay (ELISA) although this method is not thoroughly evaluated. The objective of this study was to determine the sensitivity and specificity of an ELISA for detection...... of the ELISA methods on milk and blood were equal at 0.99. No conditional dependence was observed between the specificity estimates of the two test methods. However, the sensitivity estimates of both tests were significantly reduced when conditional covariances ≥40 were used. Collection of milk samples from...... to positive (S/P) cut-off of 40 for both blood and milk ELISAs. At this cut-off, sensitivity of milk ELISA was 0.86 (95% posterior credibility interval [PCI] [0.76; 0.96]). This was slightly but insignificantly higher than sensitivity of blood ELISA (0.84; 95% PCI [0.75; 0.93]). The specificity estimates...

  9. Evaluation of the dot enzyme-linked immunosorbent assay in comparison with standard ELISA for the immunodiagnosis of human toxocariasis

    Directory of Open Access Journals (Sweden)

    Roldán William

    2006-01-01

    Full Text Available A dot enzyme-linked immunosorbent assay (dot-ELISA was standardized using excretory-secretory antigens of Toxocara canis for the rapid immunodiagnosis of human toxocariasis. Thirty patients with clinical signs of toxocariasis, 20 cases with other parasitic diseases, and 40 healthy subjects were tested. A total of 0.2 ng of antigen per dot, serum dilution of 1:160 and dilution conjugate of 1:1000 were found optimal. The sensitivity and specificity of the assay were 100 and 95%, respectively. Comparable sensitivity of dot-ELISA and the standard ELISA was obtained, but only 3 cross-reactions occurred in the dot-ELISA, compared with 6 in the standard ELISA. Dot-ELISA is simple to perform, rapid, and low cost. Large-scale screening studies should be done to evaluate its usefulness under field conditions.

  10. Avaliação do desempenho do método do ensaio por enzimas imuno-adsorvidas (ELISA em leite em pó reconstituído contaminado experimentalmente com aflatoxina M1 Evaluation of competitive enzyme-linked immunosorbent assay (ELISA in milk powder contaminated with known concentrations of aflatoxin M1

    Directory of Open Access Journals (Sweden)

    Carlos Augusto Fernandes de Oliveira

    1996-12-01

    Full Text Available O desempenho do ensaio por enzimas imuno-adsorvidas (ELISA, mediante o emprego de conjuntos de reativos produzidos em escala comercial, para determinação de aflatoxina, foi avaliado em condições experimentais, através de análises repetidas, em amostras de leite em pó reconstituído contaminadas com concentrações conhecidas da fração M1 da toxina. Para os níveis de 0,10; 0,20; 0,50; e, 1,00 ng/ml, os percentuais de recuperação foram: 83,0%; 87,5%; 103,0%; e, 111,8%, respectivamente. O desvio-padrão relativo, para as referidas concentrações, foi, respectivamente, 65,5%; 31,8%; 10,9% e 13,6% (n=10, para cada nível de contaminação. Os resultados obtidos demonstram que o método é apropriado para pesquisas e levantamentos sobre a ocorrência de aflatoxina M1 em leite, sobretudo nas faixas de concentração entre 0,20 - 1,00 ng/ml.The evaluation of commercially available test systems of competitive enzyme-linked immunosorbent assay (ELISA, for Aflatoxin M1 (AFM1 was performed in experimental conditions, through repeated analysis, in samples of milk powder contamined with known concentrations of the toxin. Recoveries of AFM1 added to milk at levels of 0.10, 0.20, 0.50 and 1.00 ng/ml were 83.0%, 87.5%, 103.0% and 111.8% respectively. Relative standard deviations for the above mentioned concentrations were 65.5%, 31.8%, 10.9% and 13.6%, respectively (n =10, per spiking level. According to these results the ELISA is appropriate for AFM1 research and/or surveying, mainly for concentrations between 0.20 - 1.00 ng/ml.

  11. Jatobal virus antigenic characterization by ELISA and neutralization test using EIA as indicator, on tissue culture

    Directory of Open Access Journals (Sweden)

    Luiz Tadeu M. Figueiredo

    1988-06-01

    Full Text Available A virus antigenic characterization methodology using an indirect method of antibody detection ELISA with virus-infected cultured cells as antigen and a micro virus neutralisation test using EIA (NT-EIA as an aid to reading were used for antigenic characterization of Jatobal (BeAn 423380. Jatobal virus was characterized as a Bunyaviridae, Bunyavirus genus, Simbu serogroup virus. ELISA using infected cultured cells as antigen is a sensitive and reliable method for identification of viruses and has many advantages over conventional antibody capture ELISA's and other tests: it eliminates solid phase coating with virus and laborious antigen preparation; it permits screening of large numbers of virus antisera faster and more easily than by CF, HAI, or plaque reduction NT. ELISA and NT using EIA as an aid to reading can be applicable to viruses which do not produce cytopathogenic effect. Both techniques are applicable to identification of viruses which grow in mosquito cells.A caracterização antigênica do vírus Jatobal (BeAn 423380 foi efetuada utilizando uma técnica de ELISA para deteccão de anticorpos que utiliza culturas celulares infectadas como antígeno e um micro teste de neutralização para vírus que utiliza o método imunoenzimático como auxiliar para a leitura dos resultados (NT-EIA. O vírus Jatobal foi caracterizado como um Bunyaviridae, gênero Bunyavirus, pertencente ao sorogrupo Simbu. A técnica de ELISA, utilizando culturas celulares infectadas como antígeno, trata-se de método sensível e confiável na identificação de agentes virais, possuindo muitas vantagens sobre ELISA convencionais e outros testes: elimina a preparação laboriosa de antígenos para o revestimento em fase sólida; permite que se teste de forma mais rápida e fácil que por CF, HAI e neutralização por redução de plaques um grande número de antisoros de vírus. ELISA e NT-EIA podem ser utilizados para a classificação de vírus que não produzem

  12. Capture ELISA for IgM antibodies against Plasmodium falciparum glutamate rich protein

    DEFF Research Database (Denmark)

    Dziegiel, Morten Hanefeld; Borre, M B; Petersen, E;

    1992-01-01

    This report describes a novel mu chain capture ELISA for the detection of IgM antibodies against a Plasmodium falciparum antigen. A fragment of the 220 kDa P. falciparum glutamate rich protein containing amino acid residues 489-1271 was expressed in E. coli as a recombinant chimeric beta-galactos......This report describes a novel mu chain capture ELISA for the detection of IgM antibodies against a Plasmodium falciparum antigen. A fragment of the 220 kDa P. falciparum glutamate rich protein containing amino acid residues 489-1271 was expressed in E. coli as a recombinant chimeric beta...

  13. Microcapillary blood sampling for serological examinations by radioimmunoassay (RIA) and enzyme immunoassay (ELISA)

    Energy Technology Data Exchange (ETDEWEB)

    Rodak, L.; Smid, B.; Valicek, L.; Jurak, E. (Vyzkumny Ustav Veterinarniho Lekarstvi, Brno-Medlanky (Czechoslovakia))

    1984-01-01

    Methods were tested of sampling blood and blood serum for serological examinations on filtration paper and into heparinized glass capillaries with transfer into the dilution solution of the given composition. Samples were also examined for ACH virus antibodies. The suitability of the sampling was verified by an examination of samples using ELISA and RIA methods. The results showed the suitability of sampling using microcapillaries. The titres of virus antibodies found using the ELISA and RIA methods were identical and the sensitivity of antibody detection was not reduced even after the sample had been stored for 60 days at a temperature of 20 degC.

  14. Construction of an optical test-bed for eLISA

    OpenAIRE

    Lieser, M.; Fitzsimons, E; Isleif, K.S.; Killow, C; Perreur-Lloyd, M; Roberstson, D.; Schuster, S.; Trobs, M.; Veith, S.; Ward, H; Heinzel, G.; Danzmann, K.

    2016-01-01

    In the planned eLISA mission a key part of the system is the optical bench that holds the interferometers for reading out the inter-spacecraft distance and the test mass position. We report on ongoing technology development for the eLISA optical system like the back-link between the optical benches and the science interferometer where the local beam is interfered with the received beam from the distant spacecraft. The focus will be on a setup to investigate the tilt-to-pathlength coupling in ...

  15. Extension of the four-parameter logistic model for ELISA to multianalyte analysis.

    Science.gov (United States)

    Jones, G; Wortberg, M; Kreissig, S B; Bunch, D S; Gee, S J; Hammock, B D; Rocke, D M

    1994-12-28

    The standard implementation of enzyme-linked immunosorbent assay (ELISA) for single analytes can lead to false conclusions if cross reacting compounds are present in the sample. This paper discusses the extension of the usual four-parameter logistic model for ELISA to the case of multiple cross-reacting analytes. The use of the extended model in multianalyte analysis (MELISA) is illustrated and compared with a more simplistic approach. Data on the analysis of a binary mixture of s-triazines suggests the superiority of the proposed model. This model is also suitable for other forms of immunoassay that use the four-parameter logistic curve. PMID:7822815

  16. Validation and use of an ELISA kit for the diagnosis of Babesia bovis in Cuba

    International Nuclear Information System (INIS)

    Babesia bovis, the most important etiological agent causing bovine babesiosis, is widely distributed in Cuba and affects mainly adult cattle. A survey of the prevalence of the disease in cattle using an ELISA kit (FAO/IAEA) revealed that 34.2% of the animals between 6 and 18 months of age were positive to Babesia bovis, whereas 69.9% on the cattle older than 18 months were positive. Antibodies to Babesia bovis were detected in 96.9% of calves vaccinated with an attenuated Babesia bovis vaccine. A good correlation was found between the results of ELISA kit with those from indirect immunofluorescence and immunoperoxidase tests developed in Cuba. (author)

  17. Serodiagnosis of Rift Valley fever in African wildlife using a recombinant nucleocapsid-based indirect ELISA

    International Nuclear Information System (INIS)

    Antibodies to Rift Valley fever virus (RVFV) have been found in many wildlife species but their importance in the epidemiology of the disease during the inter-epidemic and epidemic periods, and including their possible specific role in the cryptic maintenance of the virus is not elucidated. A recently developed indirect ELISA (I-ELISA) based on recombinant nucleocapsid protein (rNp) of RVF virus was reported to have high analytical accuracy for the detection of IgG antibody in African buffalo sera. An indirect ELISA (I-ELISA) based on the recombinant nucleocapsid protein (rNp) of Rift Valley fever virus (RVFV) was evaluated for the detection of specific serum IgG antibody in African wildlife. Data sets derived from field-collected sera (n = 877) in Africa (antelopes = 529, black rhinoceros = 43, common zebra = 24, elephant = 73, giraffe = 81, grevy zebra = 78, warthog = 49) were categorized according to the results of a virus neutralization test. Dose response curves using different dilutions of sera known to be positive or negative in the virus neutralisation test had the expected analytical slope and the I-ELISA clearly differentiated between different levels of specific IgG antibody against RVFV in African wildlife. At cut-offs optimised by the two-graph receiver operating characteristics analysis, the diagnostic sensitivity of the I-ELISA was 100% and diagnostic specificity ranged from 99.8% to 100% while estimates for the Youden's index (J) and efficiency (Ef) ranged from 0.99 to 1 and from 99.7% to 100%, respectively. The rNp-based I-ELISA is highly accurate, safe, and offers a single assay format for rapid detection of IgG antibody to RVFV in sera of different wildlife species. This study confirm previous findings that the rNp-based I-ELISA accurately identifies sera with different concentrations of specific IgG antibodies to RVF virus, and compared to virus neutralization test it has very high diagnostic performance in various wildlife animal species. As a

  18. Utilización de los test de Fluorescencia Polarizada (FP) y Elisa de Competencia (C-Elisa) en el diagnóstico de brucelosis de camélidos The use of polarized fluorescence assay (PF) and competitive ELISA test (C-Elisa) for the diagnosis of Brucellosis in South American camelids

    OpenAIRE

    Rojas, X.; Muñoz, S.; Otto, B.; B. Pérez; Nielsen, K.

    2004-01-01

    En Chile, como en muchos países de Latinoamérica, la brucelosis es una zoonosis presente, representando no sólo un factor de riesgo para las explotaciones pecuarias sino también para quienes laboran y hacen usufructo de ella, especialmente por el consumo de leche cruda y sus derivados. Como la Brucella afecta a diferentes especies de animales, el objetivo de este trabajo fue utilizar las técnicas de unión primaria, como son Fluorescencia Polarizada (FP) y Elisa de Competencia (C-Elisa), para ...

  19. Respuestas ante la confusión del consumidor por información ambigua: el caso de los cosméticos de una cadena de supermercados en España

    OpenAIRE

    José S. Clemente-Ricolfe; Carmen Escribá-Pérez; Juan M. Buitrago-Vera

    2014-01-01

    Este estudio expuso y agrupó las reacciones de los consumidores ante la confusión generada por información ambigua para una marca de cosméticos de una cadena de supermercados. El método de recogida de la información fue la netnografía, usando para su análisis Componentes Principales Categóricos. Este trabajo supuso una aportación a la literatura sobre la confusión del consumidor porque la información se recogió a partir de comentarios on-line. La respuesta más citada fue la mala calidad...

  20. Analisis de oportunidades de implementacion de tecnologias de la información y comunicaciones (tic's) logísticas en la cadena de suministro del oro en el tolima

    OpenAIRE

    CORREA ESPINAL, ALEXANDER; GÓMEZ MONTOYA, RODRIGO ANDRÉS

    2010-01-01

    En el presente artículo se revisan y analizan oportunidades de implementación de Tecnologías de la Información y Comunicaciones (TIC's). como medio para que la cadena del oro en el distrito minero de Bermellón en el Tolima aumente su productividad y aprovechamiento de sus recursos a través de una adecuada gestión logística. Para alcanzar, este objetivo, se realiza un revisión bibliográfica y análisis de libros e informes relacionados con el tema, los cuales cubren, desde la descripción de la ...

  1. El Síndrome de Fatiga Crónica: del modelo de susceptibilidad a la hipótesis de la cadena de estrés en el área laboral.

    Directory of Open Access Journals (Sweden)

    Fernando Mansilla Izquierdo

    2003-01-01

    Full Text Available El modelo de susceptibilidad tiene en cuenta la combinación de algunos elementos: la susceptibilidad biológica, la susceptibilidad al mobbing, la susceptibilidad al burn-out, la susceptibilidad al estrés laboral, y los factores de protección como: apoyo social, autoconcepto positivo, locus de control interno, alta autoestima y sentimiento de autoeficacia. La hipótesis de la cadena de estrés en el área laboral considera que la persistencia del estrés laboral es acumulativa. El mobbing, el síndrome de burn-out y el estrés laboral provocan distintos tipos de fatiga. Si los factores estresantes que generan estos problemas perseveran durante tiempo, la fatiga puede devenir en lo que se ha dado en llamar síndrome de fatiga crónica.

  2. Administración de la cadena de abasto de productos de consumo repetitivo en el hogar en Colombia. Aproximación a un marco histórico y teórico.

    Directory of Open Access Journals (Sweden)

    Ricardo Acevedo Trujillo

    2013-11-01

    Full Text Available Este trabajo intenta una primera aproximación teórico-histórica a la administración de la cadena de abasto en Colombia en el área de los almacenes que proveen bienes de consumo repetitivo en el hogar. El tema es pertinente puesto que el sector se encuentra en este momento en transformación, debido a la entrada de grandes capitales de la industria multinacional.   Desde la perspectiva del autor, los “superetes” entendidos como: “tiendas de barrio con caja de salida”, son una oportunidad interesante para que el pequeño comerciante colombiano enfrente la entrada de estos capitales. En este sentido el articulo plantea un marco teórico para una aproximación a  la gestión adecuada de estos establecimientos comerciales.

  3. A comparative study of an elisa test and an indirect immunofluorescence test for serological diagnosis of Babesia bovis infection

    International Nuclear Information System (INIS)

    Detection of antibodies to Babesia bovis in cattle is essential for the understanding of the epidemiology of babesiosis and this study was concerned with comparing the indirect fluorescent antibody with the ELISA. Both assays gave rise to 100% sensitivity whilst the ELISA was shown to be marginally more specific at 98%. The ease of use and low cost of the ELISA would make it the more obvious choice in conducting future serological surveys for this parasite. (author)

  4. Determinación de la difusividad térmica del subsuelo mediante medidas de temperaturas.

    OpenAIRE

    García Arias, Sandra

    2014-01-01

    El objetivo del presente trabajo será calcular la difusividad térmica del terreno siguiendo el modelo de transmisión de calor a través de la superficie con un flujo periódico perpendicular a ella. Se realiza en base a un experimento que conlleva la realización de un sondeo de 2 m de profundidad en el Campus de Mieres donde se introducirá una cadena de sensores que registrarán las temperaturas a distintas profundidades del sondeo. Se ha diseñado un equipo de registro de datos de temperatur...

  5. Determination of polychlorinated biphenyls (PCBs) in insulating oil by cocktail PCB ELISA

    Energy Technology Data Exchange (ETDEWEB)

    Goda, Y.; Hirobe, M.; Fujimoto, S. [Japan EnviroChemicals Ltd., Tokyo (Japan); Tomita, J.; Nishikiori, M.; Nakajima, T. [Tokyo Electric Power Environmental Engineering Co. Inc., Tokyo (Japan); Rubio, F. [Abraxis LLC, Warminster, PA (United States); Takigami, H. [National Inst. for Environmental Studies, Tsukuba (Japan); Sakai, S. [Kyoto Univ., Kyoto (Japan). Environment Preservation Centre; Ike, M.; Fujita, M. [Osaka Univ., Osaka (Japan). Graduate School of Engineering

    2005-07-01

    This paper described a novel cocktail polychlorinated biphenyl (PCB) enzyme-linked immunosorbent assay (ELISA) designed as a simple pretreatment method for the determination of PCBs in insulating oils. Oil samples were treated with hexane and dimethyl sufloxide (DMSO) and mixed. Hexane layers were then removed, and freshly prepared hexane was added. The DMSO layer was then diluted with an aqueous solution and extracted with hexane. After being dehydrated, the hexane layer was sulfonated with a sulfuric reagent and washed. Standard or pretreated PCBs were then dissolved and diluted in 100 per cent DMSO and added to distilled water prior to the ELISA assay. A horseradish peroxidase conjugate and an anti-PCB antibody coupled with magnetic particles were added for the assay. A magnetic rack was used to separate the reaction mixture. A colouring reagent was then added. A total of 26 insulating oils contaminated with PCBs were determined using the new cocktail method and compared with data obtained from traditional gas chromatograph electron capture detector (GC-ECD) data. Results showed that the values of both the cocktail and general PCB ELISA were well correlated with the CG-ECD data. It was concluded that combining cocktail PCB ELISA with the developed pretreatment method provided accurate results. 3 refs., 4 tabs., 2 figs.

  6. Detection of Q Fever Specific Antibodies Using Recombinant Antigen in ELISA with Peroxidase Based Signal Amplification

    Directory of Open Access Journals (Sweden)

    Hua-Wei Chen

    2014-01-01

    Full Text Available Currently, the accepted method for Q fever serodiagnosis is indirect immunofluorescent antibody assay (IFA using the whole cell antigen. In this study, we prepared the recombinant antigen of the 27-kDa outer membrane protein (Com1 which has been shown to be recognized by Q fever patient sera. The performance of recombinant Com1 was evaluated in ELISA by IFA confirmed serum samples. Due to the low titers of IgG and IgM in Q fever patients, the standard ELISA signals were further amplified by using biotinylated anti-human IgG or IgM plus streptavidin-HRP polymer. The modified ELISA can detect 88% (29 out of 33 of Q fever patient sera collected from Marines deployed to Iraq. Less than 5% (5 out of 156 of the sera from patients with other febrile diseases reacted with the Com1. These results suggest that the modified ELISA using Com1 may have the potential to improve the detection of Q fever specific antibodies.

  7. Serodiagnosis of neurocysticercosis in patients with epileptic seizure using ELISA and immunoblot assay.

    Science.gov (United States)

    Ishida, Maria M I; Peralta, Regina Helena S; Livramento, José A; Hoshino-Shimizu, Sumie; Peralta, José M; Vaz, Adelaide J

    2006-01-01

    Sera from 88 patients from Santa Catarina and São Paulo states of Brazil, with epileptic seizures who underwent cerebral computed tomography (CT) were analyzed for the detection of antibodies to T. solium cysticercus by ELISA and Immunoblot (IB) with the following antigens: Taenia solium cysticercus total saline (Tso), Taenia crassiceps cysticercus vesicular fluid (Tcra-vf) and T. crassiceps cysticercus glycoproteins (Tcra-gp). ELISA carried out with Tso, Tcra-vf and Tcra-gp antigens showed 95%, 90% and 80% sensitivities, respectively, and 68%, 85% and 93% specificities, respectively. In the epileptic patients group, ELISA positivity was 30%, 51% and 35% with Tso, Tcra-vf and Tcra-gp antigens respectively. Considering the IB as the confirmatory test, the positivity was 16% (14/88) in the epileptic patients total group and 22% (12/54) in the epileptic patients with positive CT and signals of cysticercosis. We found a significant statistical correlation among ELISA or IB results and the phase of the disease when any antigens were used (p neurocysticercosis (NC) in patients presenting with epileptic seizures because of the high risk of acquiring NC in our region and its potential cause of epilepsy.

  8. QUANTITATIVE ELISA OF POLYCHLORINATED BIPHENYLS IN AN OILY SOIL MATRIX USING SUPERCRITICAL FLUID EXTRACTION

    Science.gov (United States)

    Soil samples from the GenCorp Lawrence Brownfields site were analyzed with a commercial semi-quantitative enzyme-linked immunosorbent assay (ELISA) using a methanol shake extraction. Many of the soil samples were extremely oily, with total petroleum hydrocarbon levels up to 240...

  9. Agricultural production - Phase 2. Indonesia. Implementation of ELISA for brucellosis at DGLS laboratories in indonesia

    International Nuclear Information System (INIS)

    This expert mission was performed to assess the current status of activities relating to brucellosis testing at the Regional Disease Investigation Centres of the Directorate General of Livestock Services in Indonesia, and to assist in the establishment and validation of the ELISA technique for detecting Brucella antibodies

  10. Agricultural production - Phase 2. Indonesia. National training course on ELISA for seradiagnosis of animal diseases (5)

    International Nuclear Information System (INIS)

    This report describes the content of a three-week national training course for 16 participants from regional Disease Investigation Centres and other agencies in Indonesia. The subject of the course was the use of ELISA for the diagnosis of animal diseases in Indonesia, with particular emphasis placed on bovine brucellosis

  11. Parametric analysis of a novel semi-circular microfluidic CD-ELISA valve

    Directory of Open Access Journals (Sweden)

    Lin Samuel

    2011-11-01

    Full Text Available Abstract CD-ELISA uses the microfluidic ranking method and centrifugal force to control the testing solution as it flows into the reaction region. The most challenging part of CD-ELISA is controlling the flow process for different biological testing solutions, i.e. the controlling sequence for the microfluidic channel valves. The microfluidic channel valve is therefore the most important fluid channel structure for CD-ELISA. In this study, we propose a valve design suitable for a wide range rotational speeds which can be applied for mass production (molding. Together with supporting experiments, simulation based on two-phase flow theory is used in this study, and the feasibility of this novel valve design is confirmed. Influencing design factors for the microfluidic channel valves in CD-ELISA are investigated, including various shapes of the arc, distance d, radius r, the location of the center of the circle, and the contact angle. From both the experimental results and the simulated results, it is evident that the narrowest channel width and the contact angle are the primary factors influencing valve burst frequency. These can be used as the main controlling factors during the design.

  12. Evaluation of the ELISA test for detection of Entamoeba histolytica in feces.

    Science.gov (United States)

    Merino, E; Glender, W; del Muro, R; Ortiz-Ortiz, L

    1990-01-01

    The clinical utility of an ELISA test with monoclonal antibodies to detect antigen of Entamoeba histolytica in feces was evaluated in 150 patients with gastrointestinal symptoms. Each subject was examined by rectosigmoidoscopy with rectal smear and/or a triple stool search for ova-bacteria-parasite (OBP); in addition, one stool sample was collected for the ELISA test. All the tests were independent and double blind. E. histolytica was detected by OBP and/or rectosigmoidoscopy in 66 patients; 61 patients had other parasites; and in 23, no parasites were identified. Of all patients, 116 were positive for the ELISA test. Of these, E. histolytica was identified in 52. In 47, other parasites were identified and in 17, no parasites were found. The ELISA test with a monoclonal antibody against E. histolytica antigen showed higher sensitivity than the standard diagnostic methods: the ability to detect the presence of E. histolytica antigen regardless of the destruction of the parasite or of the error due to misidentification of the parasite resulting from faulty preparation of the samples.

  13. Qualitative and quantitative detection of T7 bacteriophages using paper based sandwich ELISA.

    Science.gov (United States)

    Khan, Mohidus Samad; Pande, Tripti; van de Ven, Theo G M

    2015-08-01

    Viruses cause many infectious diseases and consequently epidemic health threats. Paper based diagnostics and filters can offer attractive options for detecting and deactivating pathogens. However, due to their infectious characteristics, virus detection using paper diagnostics is more challenging compared to the detection of bacteria, enzymes, DNA or antigens. The major objective of this study was to prepare reliable, degradable and low cost paper diagnostics to detect viruses, without using sophisticated optical or microfluidic analytical instruments. T7 bacteriophage was used as a model virus. A paper based sandwich ELISA technique was developed to detect and quantify the T7 phages in solution. The paper based sandwich ELISA detected T7 phage concentrations as low as 100 pfu/mL to as high as 10(9) pfu/mL. The compatibility of paper based sandwich ELISA with the conventional titre count was tested using T7 phage solutions of unknown concentrations. The paper based sandwich ELISA technique is faster and economical compared to the traditional detection techniques. Therefore, with proper calibration and right reagents, and by following the biosafety regulations, the paper based technique can be said to be compatible and economical to the sophisticated laboratory diagnostic techniques applied to detect pathogenic viruses and other microorganisms.

  14. ELISA and other methods in monitoring vaccination response to haemorrhagic septicemia

    International Nuclear Information System (INIS)

    Two groups each of 15 buffaloes were vaccinated subcutaneously or intramuscularly with a Pasteurella multocida vaccine consisting of crude capsular antigen. No significant differences in immune response were observed between the two groups using the indirect haemagglutination assay. Sera from the vaccinated buffaloes were subjected to the passive mouse protection test and there was an overall average survival rate of 87.5% when the mice were challenged with 100LD50 of the homologous vaccine strain. In a second trial, 67 buffaloes were grouped into 7 groups. Six of the groups were vaccinated with whole cell or crude capsular antigen respectively combined with water, alum or an oil adjuvant respectively. The seventh group (group VII) was not vaccinated and served as the control. Sera collected from these groups were tested by an enzyme-linked immunosorbent assay (ELISA) and the results obtained analyzed by Duncan's multiple range test. The antibody responses of vaccinated groups were statistically different to the control group. Serum from these buffaloes were grouped according to their ELISA optical density values (x dilution factor) and inoculated into mice. One hundred percent protection was provided by sera with an ELISA titre between 71-90 collected 3 months after vaccination while 11 months after vaccination 100% protection was provided but only with sera having ELISA titres greater than 90. (author). 9 refs, 3 figs, 2 tabs

  15. Combined use of Western blot/ELISA to improve the serological diagnosis of human tuberculosis

    Directory of Open Access Journals (Sweden)

    S. T. Beck

    2005-02-01

    Full Text Available Two recombinant antigens and a crude bacterial antigen of a wild M. tuberculosis strain were used to detect specific IgG antibodies in sera from 52 patients with pulmonary tuberculosis, confirmed by an acid-fast smear and serum culture of these patients and that of 25 contacts. The patients were not infected with HIV. We evaluated the sensitivity and specificity of ELISA, based on the recombinant TbF6® and TbF6/DPEP antigen and a search for reactivity patterns in the Western blot technique, using whole mycobacterium antigen. Serum samples from 22 healthy individuals and from 30 patients with lung diseases other than tuberculosis were used as controls. The best ELISA results were obtained with the TbF6/DPEP antigen combination, which gave 85% sensitivity and 91% specificity. ELISA sensitivity improved from 85% to 92% when the Western blot results were used. Western blot specificity was 100% when antibody reactivity with different antigenic bands was analyzed and associated. The association of TbF6/DPEP antigens used in ELISA with specific patterns of reactivity determined by Western blot can help make an identification when classic methods for the diagnosis of pulmonary tuberculosis are not sufficient.

  16. Evaluation of an ELISA for the routine diagnosis of Dictyocaulus viviparus infections in cattle

    NARCIS (Netherlands)

    Cornelissen, J.B.W.J.; Borgsteede, F.H.M.; Milligen, van F.J.

    1997-01-01

    An enzyme-linked immunosorbent assay (ELISA) that detects antibodies against Dictyocaulus viviparus in experimentally and naturally infected cattle was evaluated for its sensitivity, specificity, the moment of seroconversion and persistence of the anti-D. viviparus response and precision. The first

  17. Rapid Detection of Food Allergens by Microfluidics ELISA-Based Optical Sensor

    Directory of Open Access Journals (Sweden)

    Xuan Weng

    2016-06-01

    Full Text Available The risks associated with the presence of hidden allergens in food have increased the need for rapid, sensitive, and reliable methods for tracing food allergens in commodities. Conventional enzyme immunosorbent assay (ELISA has usually been performed in a centralized lab, requiring considerable time and sample/reagent consumption and expensive detection instruments. In this study, a microfluidic ELISA platform combined with a custom-designed optical sensor was developed for the quantitative analysis of the proteins wheat gluten and Ara h 1. The developed microfluidic ELISA biosensor reduced the total assay time from hours (up to 3.5 h to 15–20 min and decreased sample/reagent consumption to 5–10 μL, compared to a few hundred microliters in commercial ELISA kits, with superior sensitivity. The quantitative capability of the presented biosensor is a distinctive advantage over the commercially available rapid methods such as lateral flow devices (LFD and dipstick tests. The developed microfluidic biosensor demonstrates the potential for sensitive and less-expensive on-site determination for rapidly detecting food allergens in a complex sample system.

  18. Applicability of ELISA-based Determination of Pesticides for Groundwater Quality Monitoring

    Science.gov (United States)

    Tsuchihara, Takeo; Yoshimoto, Shuhei; Ishida, Satoshi; Imaizumi, Masayuki

    The principals and procedures of ELISA (Enzyme-linked Immunosorbent Assay)-based determination of pesticides (Fenitrothion) in environmental samples were reviewed, and the applicability of the ELISA method for groundwater quality monitoring were validated through the experimental tracer tests in soil columns and the field test in Okinoerabu Island. The test results showed that the ELISA method could be useful not only for screening but also for quantitative analysis of pesticides. In the experimental tracer tests in soil columns, the retardation of pesticides leaching compared with conservative tracers were observed. In the field test, the contamination of the pesticide was detected in groundwater samples in Okinoerabu Island, even though the targeted pesticide was considered to be applied to the upland field 4 months ago. In order to investigate the transport and fate of pesticides in groundwater taking into account retardation from the field to groundwater table and the residue in groundwater, continuous observations of pesticides in groundwater are in a strong need, and the ELISA method is applicable to the long-term quality groundwater monitoring.

  19. Correlation between ELISA and ML Flow assays applied to 60 Brazilian patients affected by leprosy

    NARCIS (Netherlands)

    R.C. Da Silva; S. Lyon; A.C. Lyon; M.A.F. Grossi; S.H. Lyon; S. Buhrer-Sekula; C.M.F. Antunes

    2010-01-01

    Serological tests can be helpful in classifying leprosy patients as having either the paucibacillary or the multibacillary form. The aim of this study was to evaluate the concordance between two serological assays, i.e. ML Flow and ELISA, in a population of leprosy patients in Brazil. The investigat

  20. Rapid Detection of Food Allergens by Microfluidics ELISA-Based Optical Sensor.

    Science.gov (United States)

    Weng, Xuan; Gaur, Gautam; Neethirajan, Suresh

    2016-01-01

    The risks associated with the presence of hidden allergens in food have increased the need for rapid, sensitive, and reliable methods for tracing food allergens in commodities. Conventional enzyme immunosorbent assay (ELISA) has usually been performed in a centralized lab, requiring considerable time and sample/reagent consumption and expensive detection instruments. In this study, a microfluidic ELISA platform combined with a custom-designed optical sensor was developed for the quantitative analysis of the proteins wheat gluten and Ara h 1. The developed microfluidic ELISA biosensor reduced the total assay time from hours (up to 3.5 h) to 15-20 min and decreased sample/reagent consumption to 5-10 μL, compared to a few hundred microliters in commercial ELISA kits, with superior sensitivity. The quantitative capability of the presented biosensor is a distinctive advantage over the commercially available rapid methods such as lateral flow devices (LFD) and dipstick tests. The developed microfluidic biosensor demonstrates the potential for sensitive and less-expensive on-site determination for rapidly detecting food allergens in a complex sample system. PMID:27338488

  1. Diagnosis of bovine brucellosis in Mosul city by indirect ELISA and conventional serological tests

    Directory of Open Access Journals (Sweden)

    O. KH. AL-Hankawe

    2010-01-01

    Full Text Available The study was conducted on 126 cattle (94 females and 32 males of different ages (1->5 years randomly selected from July 2007 to August 2008 in Mosul. Indirect ELISA test and other traditional tests (rose Bengal test, tube agglutination test and 2- mercapto-ethanol test were used to determine the incidence of bovine brucellosis. The highest incidence of disease was recorded by Indirect ELISA, 23.01%, whereas it was 18.25%, 11.90% and 4.76% by rose Bengal, tube agglutination and 2- Mercapto-ethanol tests, respectively. The highest incidence was in females in all serological tests and the highest incidence was in females at the age between 1-3 years whereas in males more than 3 years of age it was 23.07%. The results of tube agglutination test revealed the titer 1/40 occurred mostly compared with other titers. Six chronic cases were determined by 2-mercapto-ethanol test. The degree of agreement of negative samples with rose Bengal test and indirect ELISA, tube agglutination, and 2- mercapto-ethanol tests was 94.17%, 100% and 100%, respectively, and by indirect ELISA with rose Bengal, tube agglutination and 2-mercapto-ethanol tests was 79.31%, 51.72% and 20.68%, respectively.

  2. DETERMINATION OF 3,5,6-TRICHLORO-2-PYRIDINOL (TCP) BY ELISA

    Science.gov (United States)

    A sensitive, competitive enzyme-linked immunosorbent assay (ELISA) for 3,5,6-trichloro-2pyridinol (TCP) has been developed to quantitate parts per billion (ppb) amounts of the analyte in urine. TCP is a major metabolite and environmental degradation product of the insecticide c...

  3. Combined use of random access and ELISA analyzers in the microbiological serology laboratory

    OpenAIRE

    Alessandra Moroni; Antonella Marangoni; Marina Biagi; Silvia Capitani; Enzo Della Bella; Sanzio Ruscello; Franca Savioli; Roberto Cevenini

    2008-01-01

    In the last years the trend of centralizing small laboratories in large reference centers led to a careful evaluation of the diagnostic profiles. In the serology laboratory of Microbiology Unit, St. Orsola-Malpighi Hospital, Bologna, Italy the choice has been to combine random access analyzers (ARCHITECT Abbott) and ELISA analyzers (BEPIII Dade Behring).

  4. Combined use of random access and ELISA analyzers in the microbiological serology laboratory

    Directory of Open Access Journals (Sweden)

    Alessandra Moroni

    2008-09-01

    Full Text Available In the last years the trend of centralizing small laboratories in large reference centers led to a careful evaluation of the diagnostic profiles. In the serology laboratory of Microbiology Unit, St. Orsola-Malpighi Hospital, Bologna, Italy the choice has been to combine random access analyzers (ARCHITECT Abbott and ELISA analyzers (BEPIII Dade Behring.

  5. Validation of a multiplex reverse transcriptase PCR ELISA for the detection of 19 respiratory tract pathogens

    NARCIS (Netherlands)

    Puppe, W.; Weigl, J.; Groendahl, B.; Knuf, M.; Rockahr, S.; von Bismarck, P.; Aron, G.; Niesters, H. G. M.; Osterhaus, A. D. M. E.; Schmitt, H. -J.

    2013-01-01

    Introduction Since acute respiratory tract infections inflict a high burden of disease in children worldwide, a multiplex reverse transcription polymerase chain reaction combined with a microwell hybridization assay (m-RT-PCR ELISA) to detect 19 different respiratory pathogens was developed and vali

  6. RT-PCR-ELISA as a tool for diagnosis of low-pathogenicity avian influenza

    DEFF Research Database (Denmark)

    Dybkaer, Karen; Munch, Mette; Handberg, Kurt Jensen;

    2003-01-01

    A one-tube reverse transcriptase/polymerase chain reaction coupled with an enzyme-linked immunosorbent assay (RT-PCR-ELISA) was developed for the rapid detection of avian influenza virus (AIV) in clinical specimens. A total of 419 swab pools were analyzed from chickens experimentally infected...

  7. Evaluation of commercial ELISA kits for detection of antibodies against bovine atypical pestivirus

    DEFF Research Database (Denmark)

    Larska, Magdalena; Polak, Mirosław P.; Uttenthal, Åse;

    A group of emerging bovine pestiviruses becomes a possible threat to Bovine Viral diarrhea virus (BVDV) control and eradication programs in the countries of their origin and in the new continents due to the lack of validated detection methods. The use of ELISA kits may be acheaper, time saving...

  8. Use of the ELISA in studies on tropical theileriosis in Morocco

    International Nuclear Information System (INIS)

    An enzyme-linked immunosorbent assay (ELISA) was developed for use in sero-epidemiological studies of theileriosis caused by Theileria annulata in Morocco. The antigen used was prepared from sonicated whole purified piroplasmas. The ELISA was used to study the serological responses in calves which where experimentally immunized with a schizont-infected attenuated cell line and challenged a month later with a lethal dose of sporozoites. The test was also used to study post vaccinal antibodies in pregnant cows and in animals immunized with different doses of the attenuated cell lines at different levels of passage. Finally, the test was used to screen sear collected from cattle in an area where infection with T. annulata was endemic before and after the theileriosis season. Sera from animals in the above studies were also analyzed using an indirect fluorescent antibody test (IFAT) with T. annulata piroplasmas in order to compare the relative sensitivities of the two tests. It was found that the piroplasma stage of T. annulata was a more appropriate stage from which to prepare antigen for use in ELISA then the sporozoite or schizont stages. A marked heterogeneity in antibody response was observed in animals vaccinated using an attenuated cell line vaccine, however, little difference in this response was seen between exotic and local animals which had not been previously exposed to infection with T. annulata. A concordance level of 91.5% was found between diagnosis by blood smears and ELISA. (author). 9 refs, 4 figs, 2 tabs

  9. A recombinant nucleocapsid-based indirect ELISA for serodiagnosis of Rift Valley fever in African wildlife

    International Nuclear Information System (INIS)

    An indirect ELISA (I-ELISA) based on the recombinant nucleocapsid protein (rNp) of Rift Valley fever virus (RVFV) was evaluated for the detection of specific serum IgG antibody in African wildlife. Data sets derived from field-collected sera (n = 918) in Africa (antelopes = 570, black rhinoceros = 43, common zebra = 24, elephant = 73, giraffe = 81, grevy zebra = 78, warthog = 49) were categorised according to the results of a virus neutralisation test (VNT). At cut-offs optimised by the two-graph receiver operating characteristics analysis, the diagnostic sensitivity of the I-ELISA was 100% and diagnostic specificity ranged from 99.8% to 100% while estimates for the Youden's index (J) and efficiency (Ef) ranged from 0.99 to 1 and from 99.7% to 100%, respectively. The rNp-based I-ELISA is highly accurate, safe, and offers a single assay format for rapid detection of IgG antibody to RVFV in sera of different wildlife species. (author)

  10. Development of hen antihepatitis B antigen IgY-based conjugate for ELISA assay

    Directory of Open Access Journals (Sweden)

    Najat Muayed Nafea

    2015-01-01

    Conclusions: This study showed that laying hens can be used as an alternative source for production of polyclonal antibodies against HBsAg and anti-HBs IgY could be labeled with HRP enzyme and could subsequently be used successfully as secondary antibody in ELISA for detection of HBsAg in the patients sera.

  11. Evaluation of an indirect ELISA for the diagnosis of Babesia bovis in Uruguay

    International Nuclear Information System (INIS)

    In initially establishing the FAO/IAEA indirect ELISA for the detection of antibodies to Babesia bovis, the optical density (OD) values of sera from known positive or negative local cattle were compared to the OD values obtained from the negative and positive reference sera provided with the ELISA kit. The percentage of false positive and negative sera were 2.53% and 2.97% respectively. The cut-off values for the negative reference serum in the kit were compared with those of a local negative population. These values were found to be similar. The specificity of the test was evaluated by testing 30 sera from animals experimentally infected with Anaplasma marginale and 30 sera from animals infected with Babesia bigemina. These were no cross-reaction either between A. marginale and B. bovis or between B. bigemina and B. bovis. A serological survey using this ELISA kit was carried out on animals from an enzootic area and an area free from the vecot Boophilus microplus. 53 out of 282 animals (18.8%) in the enzootic area were positive whilst all the animals (113) from the free area were negative. This study would indicate that the FAO/IAEA ELISA kit has a sensitivity of around 98% and specificity of 97%. (author). 8 refs, 2 figs, 2 tabs

  12. Validation of an indirect ELISA for the diagnosis of Babesia bovis in cattle in Yucatan, Mexico

    International Nuclear Information System (INIS)

    The ELISA kit provided by the FAO/IAEA for the diagnosis of Babesia bovis was validated. In order to determine the appropriate ELISA cut-off point that would serve as the threshold between positive and negative samples, 119 serum samples from a Mexican Babesia-free zone were analyzed. The optimal cut-off point chosen was at 12% of the reactivity of the high positive control serum sample (PP) which resulted in a specificity of 97%. One hundred and ninety-six cattle from Wisconsin, USA, were introduced into Yucatan, Mexico, of which 181 were vaccinated with an attenuated live Babesia bovis vaccine; 15 animals remained as unvaccinated controls. Before and after vaccination all animals were bled and tested by enzyme linked immunosorbent assay (ELISA) and indirect fluorescence antibody test (IFAT). Both tests showed a high degree of correlation in their results. To evaluate an immune response to vaccination the optimal cut-off point chosen was 12% PP resulting in a sensitivity 99% and a specificity 95%. We concluded that the ELISA test has proved to be useful in Yucatan, Mexico for serological surveys and monitoring the efficiency o vaccination programmes. (author)

  13. International ring trial to detect anti-Trichinella IgG by ELISA on pig sera.

    Science.gov (United States)

    In the present study, the sensitivity and specificity of the ELISA assay determined by the CRLP validation was 100% and 98.29%, respectively. The assay was reproducible, moreover, based on the receiver-operator characteristic (ROC) curve, the sensitivity and specificity of the assay reached 97.5% an...

  14. Evaluation of an indirect elisa for the diagnosis of bovine brucellosis in Patagonia, Argentina

    International Nuclear Information System (INIS)

    Control and eradication of bovine brucellosis is usually based on the serological detection of antibodies. In Argentina, the Rose Bengal test (RB) and the Buffered Plate antigen test (BPA) are the two screening test officially recognized, while the 2-mercaptoethanol test (2ME) and the Tube Agglutination test (SAT) are the confirmatory assays currently in use. In order to improve the serological diagnosis of bovine brucellosis in Patagonia, Argentina, an indirect ELISA kit produced by the Joint FAO/IAEA Division was evaluated. Sera from negative non-vaccinated, negative but vaccinated and positive animals were tested by all the above techniques. The specificity of the I-ELISA (99.6% and 99.7%) was similar to that of the BPA, RB, 2ME and Complement Fixation test (CF) when used to test sera from non-vaccinated, negative and vaccinated, negative animals, respectively. The sensitivity of the I-ELISA (98%) was higher than the BPA test (96%) and the CF test (95,2%). The I-ELISA kit evaluated in this study was thought to be a valuable tool for the diagnosis of bovine brucellosis in Patagonia region where little epidemiological information is available about this disease and where large numbers of sera should be tested to obtain such information. (author)

  15. Development of a sulfonamide ELISA and its comparison with LC-MS/MS method

    Science.gov (United States)

    Sulfonamides are common chemotherapeutic agents used in veterinary and human medicines to treat bacterial and protozoa infections. Because of their widespread usage sulfonamides, such as sulfamethoxazole, are some of the most prevalent pharmaceuticals found in waterways. A sulfonamide ELISA has be...

  16. Analysis of castor by ELISAs that distinguish Ricin and Ricinus communis agglutinin (RCA)

    Science.gov (United States)

    To facilitate the analysis of castor (Ricinus communis L.) seed fractions and germplasm for ricin content, we investigated the use of enzyme-linked immunosorbent assay (ELISA) methods to differentiate between ricin toxin and the related Ricinus communis agglutinin (RCA). Both proteins are based on ...

  17. A Direct, Competitive Enzyme-Linked Immunosorbent Assay (ELISA) as a Quantitative Technique for Small Molecules

    Science.gov (United States)

    Powers, Jennifer L.; Rippe, Karen Duda; Imarhia, Kelly; Swift, Aileen; Scholten, Melanie; Islam, Naina

    2012-01-01

    ELISA (enzyme-linked immunosorbent assay) is a widely used technique with applications in disease diagnosis, detection of contaminated foods, and screening for drugs of abuse or environmental contaminants. However, published protocols with a focus on quantitative detection of small molecules designed for teaching laboratories are limited. A…

  18. An ELISA for detection of antibodies against influenza A nucleoprotein in humans and various animal species.

    NARCIS (Netherlands)

    G.F. de Boer; W. Back; A.D.M.E. Osterhaus (Ab)

    1990-01-01

    textabstractA double antibody sandwich blocking ELISA, using a monoclonal antibody (MAb) against influenza A nucleoprotein (NP) was developed to detect antibodies against influenza. Collections of serum samples were obtained from human and various animal species. All influenza A subtypes induced ant

  19. ELISA versus PCR for diagnosis of chronic Chagas disease: systematic review and meta-analysis

    Directory of Open Access Journals (Sweden)

    Hasslocher-Moreno Alejandro M

    2010-11-01

    Full Text Available Abstract Background Most current guidelines recommend two serological tests to diagnose chronic Chagas disease. When serological tests are persistently inconclusive, some guidelines recommend molecular tests. The aim of this investigation was to review chronic Chagas disease diagnosis literature and to summarize results of ELISA and PCR performance. Methods A systematic review was conducted searching remote databases (MEDLINE, LILACS, EMBASE, SCOPUS and ISIWeb and full texts bibliography for relevant abstracts. In addition, manufacturers of commercial tests were contacted. Original investigations were eligible if they estimated sensitivity and specificity, or reliability -or if their calculation was possible - of ELISA or PCR tests, for chronic Chagas disease. Results Heterogeneity was high within each test (ELISA and PCR and threshold effect was detected only in a particular subgroup. Reference standard blinding partially explained heterogeneity in ELISA studies, and pooled sensitivity and specificity were 97.7% [96.7%-98.5%] and 96.3% [94.6%-97.6%] respectively. Commercial ELISA with recombinant antigens studied in phase three investigations partially explained heterogeneity, and pooled sensitivity and specificity were 99.3% [97.9%-99.9%] and 97.5% [88.5%-99.5%] respectively. ELISA's reliability was seldom studied but was considered acceptable. PCR heterogeneity was not explained, but a threshold effect was detected in three groups created by using guanidine and boiling the sample before DNA extraction. PCR sensitivity is likely to be between 50% and 90%, while its specificity is close to 100%. PCR reliability was never studied. Conclusions Both conventional and recombinant based ELISA give useful information, however there are commercial tests without technical reports and therefore were not included in this review. Physicians need to have access to technical reports to understand if these serological tests are similar to those included in

  20. ELISA, a Small Experiment For a Large Sub-millimeter Survey

    Science.gov (United States)

    Bernard, J. P.; Ristorcelli, I.; Armengaud, M.; Crussaire, J. P.; Giard, M.; Lagache, G.; Lamarre, J.-M.; Leriche, B.; Longval, Y.; Meny, C.; Stepnik, B.; Torre, J.-P.

    This paper presents the scientific objectives and technical aspects of the balloon-borne Experiment for Large Infrared Survey Astronomy (ELISA). The emphasis is put upon the synergies existing between the ELISA project and future space missions, both with respect to scientific and technical aspects. ELISA is a small balloon project for an experiment dedicated to measure the Far-Infrared to Sub-millimeter continuum emission of Galactic dust over a large fraction of the sky, with unprecedented sensitivity and angular resolution. The primary mirror of the telescope, similar to the one used for the Top-Hat mission, will have a diameter of 1 m, ensuring an angular resolution of about 3.5'. Bolometer arrays will be used in four photometric bands centered at 170, 240, 400, and 650 {μ m} and providing a 22' × 45' instantaneous field of view per channel. A liquid He cryostat will host the cold optics, including the secondary mirror of the telescope, as well as the detectors, which will be cooled to 0.3 K using an ^3He close-cycle fridge. Mapping of the sky will be accomplished by rotating the gondola over a large azimuth range (up to 60{^circ} amplitude). The pointing of the experiment will be maintained to a constant elevation during the azimuth scans through a feed back loop using the signal from a large format, fast stellar sensor, operating day and night. The scientific goal of the experiment is to map the diffuse Sub-millimeter emission along a large fraction of the Milky Way. The astronomical data obtained will be used to derive the emission properties of the dust grains in the Interstellar Medium (ISM), such as their temperature and emissivity. It will also allow to systematically measure the polarization of the dust emission. It should also lead to the detection of a few thousand point sources such as newly formed stars and distant galaxies. In addition to these goals, the ELISA project will serve as a test bed for the detector technology that will be used for the

  1. Utilización de los test de Fluorescencia Polarizada (FP y Elisa de Competencia (C-Elisa en el diagnóstico de brucelosis de camélidos The use of polarized fluorescence assay (PF and competitive ELISA test (C-Elisa for the diagnosis of Brucellosis in South American camelids

    Directory of Open Access Journals (Sweden)

    X Rojas

    2004-01-01

    Full Text Available En Chile, como en muchos países de Latinoamérica, la brucelosis es una zoonosis presente, representando no sólo un factor de riesgo para las explotaciones pecuarias sino también para quienes laboran y hacen usufructo de ella, especialmente por el consumo de leche cruda y sus derivados. Como la Brucella afecta a diferentes especies de animales, el objetivo de este trabajo fue utilizar las técnicas de unión primaria, como son Fluorescencia Polarizada (FP y Elisa de Competencia (C-Elisa, para la detección de anticuerpos anti-Brucella en suero de camélidos y, comparar sus respuestas con las pruebas de Rosa de Bengala (RB, Aglutinación Lenta en Tubo (SAT y Fijación de Complemento (FC en atención a que estas especies representan un potencial pecuario de cierta trascendencia. Para ello se analizaron 336 sueros, de los cuales 315 pertenecían a llamas y 21 a alpacas, provenientes de un predio de la Novena Región clínica y serológicamente libre de Brucella. Los resultados obtenidos señalan que el umbral de positividad para FP, calculado según la mediana del último percentil, fue de 98.7 milipolarizaciones (mP y, el corte para C-Elisa con el mismo método fue de 32% de Inhibición (% I. Todas las muestras analizadas dieron resultados negativos a RB y SAT. Los resultados con FC no pudieron ser considerados, por cuanto un alto porcentaje de las muestras fueron anticomplementarias. Con las técnicas de C-Elisa y FP, dos muestras reaccionaron positivamente en cada uno de los test con valores en el nivel de corte o levemente superiores. Se discute la significación de ellas.Brucellosis is a zoonotic disease representing a risk contact with, or consuming, their raw milk and raw dairy several animal species, including camelids, the aim of this Polarization assay (PF and Competitive ELISA (C-ELISA, along with conventional serological tests such as the Rose Complement Fixation test (CF. A total of 336 serum samples herd of the IXth Region (Chile

  2. Polymerase chain reaction and blood culture in blood donors screened by ELISA test for Chagas' disease

    Directory of Open Access Journals (Sweden)

    Andréa Tieko Kinoshita-Yanaga

    2011-03-01

    Full Text Available The objective of this study was to evaluate, through blood culture and PCR, the results of the ELISA for Chagas' disease in the screening of blood donors in the public blood-supply network of the state of Paraná, Brazil, and to map the epidemiological profile of the donors with respect to their risk of infection by Trypanosoma cruzi. The negative and positive results of the ELISA were confirmed by blood culture and PCR for 190/191 individuals (99.5%. For one individual (0.5%, the ELISA was inconclusive, blood culture and IIF were negative, and IHA and PCR positive. Three individuals (1.6% were positive for T. cruzi on all the tests. Donors were predominantly female, and natives of Paraná, of rural origin, had observed or been informed of the presence of the vector in the municipalities where they resided, had never received a blood transfusion, had donated blood 1 to 4 times, and reported no cases of Chagas' disease in their families. We concluded that PCR and blood culturing have excellent potential for confirming the results of the ELISA, and that candidate blood donors with negative or positive tests have a similar risk of infection by T. cruzi, indicating that the ELISA test is sufficiently safe for screening blood prior to use.O objetivo deste estudo foi avaliar, pela hemocultura e PCR, os resultados do teste ELISA utilizado para doença de Chagas na triagem de doadores de sangue na rede pública do Estado do Paraná, Brasil, e traçar o perfil epidemiológico dos doadores quanto ao risco de infecção pelo Trypanosoma cruzi. Os resultados negativos e positivos do ELISA foram confirmados pela hemocultura e PCR em 190/191 indivíduos (99,5%. Para um indivíduo (0,5%, o teste de ELISA foi inconclusivo, hemocultura e IFI foram negativas, HAI e PCR foram positivas. Três indivíduos (1,6% foram positivos para T. cruzi em todos os testes. A maioria dos doadores era do sexo feminino, oriundos do Estado do Paraná, de origem rural, tinham

  3. Development of fragment-specific osteopontin antibodies and ELISA for quantification in human metastatic breast cancer

    Directory of Open Access Journals (Sweden)

    Miesfeldt Susan

    2008-01-01

    Full Text Available Abstract Background Osteopontin (OPN is associated with human cancers, and circulating blood OPN may have diagnostic or prognostic value in clinical oncology. Methods To evaluate OPN as a cancer biomarker, we generated and characterized five novel mouse monoclonal antibodies against the human full-length OPN (fl-OPN. Epitopes recognized by four antibodies (2C5, 2F10, 2H9, and 2E11 map to N-terminal OPN (aa1-166; one (1F11 maps to C-terminal OPN (aa167-314. These antibodies recognize recombinant and native OPN by ELISA and immunoblot, cross reacting with human and mouse OPN. Two of these novel antibodies (2F10 and 1F11 were used to develop a quantitative enzyme linked immunosorbent assay (ELISA for fl-OPN. Results In comparison with commercially available ELISAs, our assay had high accuracy in measuring fl-OPN standards, and high sensitivity. Specifically, our ELISA has a linear dose response between 0.078 ng/ml-10 ng/ml, with a sensitivity of 13.9 pg/ml. We utilized this assay to quantify fl-OPN in the plasma of healthy volunteers in comparison with patients with metastatic breast cancer. The average circulating plasma fl-OPN in healthy volunteers was 1.2 ng/ml, compared to 4.76 ng/ml in patients with metastatic breast cancer (p = 0.0042. Although the increase in fl-OPN in cancer patients is consistent with previous studies, the measured quantity varied greatly between all existing fl-OPN ELISAs. Conclusion Because OPN is a complex molecule with diversity from alternative splicing, post-translational modification, extracellular proteolytic modification, and participation in protein complexes, we suggest that further understanding of specific isoform recognition of multiple OPN species is essential for future studies of OPN biomarker utility.

  4. Detection of Human Papillomavirus 18 in Cervical Cancer Samples Using PCR-ELISA (DIAPOPS

    Directory of Open Access Journals (Sweden)

    KN Tafreshi

    2011-12-01

    Full Text Available Background and Objectives: Human Papillomavirus (HPV infection is a major risk factor for adenocarcinoma of the cervix. The high-risk types of the virus such as HPV16 and HPV18, which possess the E6 and E7 oncogenes, are responsible for approximately 50% of all cervical cancers. A rapid, sensitive and specific test has been proposed for detection of HPV to improve cervical cancer screening programs.Objectives: The aim of this study was to develop a fast PCR-ELISA assay designated as DIAPOPS (Detection of Immobilized Amplified Products in a One Phase Systemfor detection of HPV16 and HPV18 types in SCC samples and Pap smears. The type specific primers and probes were designed for PCR and PCR-ELISA. The amplified products were hybridized with a specific biotin-labeled probe for HPV18 inner amplicons. The hybrids were detected with peroxidase conjugated avidin. The test was performed on the paraffin block and Pap smear samples from the cervical cancer patients, and the results of DIAPOPS were compared with conventional PCR assay.Results: The 70 samples (SCC and Pap smear samples were collected from Imam Khomeini and Mirzakoochak Khan Hospitals in Tehran. The PCR-based method detected six HPV16 positive, three HPV18 positive and Two HPV33 positive samples. DIAPOPS results were compared with the conventional PCR results and they showed an increase in sensitivity of the DIAPOPS test. Not only all of them were confirmed by PCR-ELISA but also three samples that conventional PCR showed negative for HPV18, were demonstrated positive by the PCR-ELISA method.Conclusion: The results of the study show that modified PCR-ELISA assay is more sensitive to detect HPV types and can be used for diagnostic purposes.

  5. Empleo del Elisa DAVIH BRU 2 en el diagnostico serologico de la Brucelosis Bovina

    Directory of Open Access Journals (Sweden)

    Carmelo Cabrera Pérez.

    2005-05-01

    Full Text Available Con el objetivo de evaluar el empleo del ELISA DAVIH BRU 2 en el diagnóstico de la brucelosis bovina en Cuba, se estudió su comportamiento en mezclas de suero frente a 872 muestras procedentes de una zona libre de la enfermedad y 14 sueros positivos bien caracterizados. También se analizó su utilidad en el estudio de un foco comparativamente con las pruebas convencionales, para lo cual se emplearon 347 muestras. Se realizó análisis económico comparativo del empleo del ELISA DAVIH BRU 2 utilizando mezclas de sueros y las pruebas convencionales de diagnóstico, teniendo en cuenta la prevalencia. El ELISA mostró una especificidad del 99,65 % al estudiarse en un área libre de brucelosis bovina empleando mezclas de suero y sin que se afectaran la sensibilidad frente a los sueros del panel estudiado. Presentó una concordancia del 100 % con la prueba Rosa de Bengala y ambas técnicas fueron las que detectaron el mayor número de animales reactores en el estudio del foco de la enfermedad. La combinación del ELISA utilizando mezclas de suero con la Reacción de Fijación del Complemento en el diagnóstico de la enfermedad, resultó el método más económico hasta una prevalencia del 2 %. Los resultados permiten recomendar la introducción del ELISA DAVIH BRU 2 en el diagnóstico serológico de la brucelosis bovina en Cuba, empleando mezclas de sueros en zonas libres de la enfermedad o con prevalencias hasta el 2 %.

  6. Assessment of an indirect ELISA for the diagnosis of bovine tuberculosis

    International Nuclear Information System (INIS)

    The sensitivity and specificity of an ELISA for the detection of bovine IgG anti-Mycobacterium bovis (M. bovis) antibodies were 73.6% and 94.1% respectively, as determined in 53 bacteriologically confirmed tuberculous cattle and 101 healthy cattle from a tuberculosis-free area. In addition, the results of ELISA and tuberculin tests in 149 cattle were compared with those of subsequent necropsy studies. Both tests failed to detect 2 animals with tuberculous lesions and positive M. bovine culture. Three of 12 cattle, in which M. bovine was isolated but no lesions were found, reacted to tuberculin as did 2 of 7 cattle that had atypical mycobacterial infection. In none of these animals was it possible to detect antibody titres against M. bovis. In 128 cattle that had no lesions nor the presence of mycobacteria, 6 were tuberculin reactors and 7 others had antibody titres against M. bovis. Negative results were obtained by ELISA in 21 of 22 paratuberculous cattle. Antibody titres were not detected in 88.9% to 96.4% of 697 cattle in three tests from two tuberculin negative herds of an endemic area. In a herd with proven M. bovis infection, the distribution of seropositive animals in tuberculin and non-tuberculin reactors was similar. Antibody responses to cutaneous tuberculin stimuli were observed in 4 cattle experimentally infected with M. bovis, but only in 2 of 10 healthy controls after repeated PPD stimuli. Nine controls which had either received a single tuberculin dose or no inoculation showed no increase in antibody levels. The low sensitivity of this ELISA limits its usefulness as a diagnostic tool for identifying individual tuberculous animals in eradication campaigns. However, it could be helpful in epidemiological surveillance if further field testing indicates that infected herds can be identified by ELISA. (author). 21 refs, 2 figs, 2 tabs

  7. Evaluating brewery wastewater toxicity using a methanogenic activity inhibition test Evaluación de la toxicidad de un efluente cervecero mediante ensayos de inhibición de la actividad metanogénica

    Directory of Open Access Journals (Sweden)

    Díaz Báez María Consuelo

    2003-12-01

    Full Text Available Anaerobic technology has been used for more than twenty years in wastewater treatment in Colombia. However, operating these systems has been complex and problems associated with operation and reactor-design have been reported. It is currently considered that the anaerobic process' stability depends on balance amongst those populations forming the anaerobic consortia and whose equilibrium could be disturbed by many factors such as toxic compounds. The aim of this study was to establish the potential toxic effect of a synthetic lubricant on methanogenic acetodastic activity by using a brewery's anaerobic sludge. A methanogenic activity inhibition test was adapted and validated to compare lubricant, penthachlorophenol and wastewater toxicity from a brewery. The concentration-response ratio, average CI50 concentration inhibition and non-observed effect concentration were established.The test showed a high level of reproducibility, the lubricant and penthachlorophenol exerted a strong inhibition of methanogenesis and a low toxic effect was observed in most wastewater samples.La tecnología anaerobia, como opción de tratamiento para las aguas residuales en Colombia, se inició a mediados de los años ochenta. Sin embargo, el manejo y operación de estos sistemas fue complejo y ha tenido un gran número de problemas, especialmente relacionados con el diseño, la puesta en marcha y la operación de los reacto­res. En la actualidad se considera que la estabilidad de un proceso anaerobio depende del balance entre las pobla­ciones que conforman el consorcio microbiano, cuyo equilibrio puede perturbarse por muchos factores, entre ellos las sustancias tóxicas. En el presente trabajo se evaluó el potencial tóxico de un lubricante sobre la actividad de las arqueas metanogénicas presentes en un lodo anaerobio. La toxicidad se determinó mediante la inhibición de la actividad metanogénica. Se estudió el efecto tóxico del pentaclorofenol (PCP, un

  8. Diagnosis of theileria equi infections in horses in the Azores using cELISA and nested PCR

    Science.gov (United States)

    Equine piroplasmosis is a tick-borne disease of equids that is often caused by the parasite Theileria equi. We applied competitive ELISA (cELISA) and nested PCR diagnostic methods to detect this parasite in horses by screening 162 samples from mainland Portugal where the parasite is endemic, and 143...

  9. A comparison of ELISA methods for the determination of human serum antibodies to Haemophilus influenzae type b

    NARCIS (Netherlands)

    van Gageldonk PGM; Mariani M; Berbers GAM; LVO-BI; Centro Ricerche; CHIRON S.p.A.; Siena; Italy/Laboratorio di Immunochimica e Sierologia Sperimentale/Departimento Immunologia

    1998-01-01

    Een vergelijking tussen de non-competitieve ELISA van Phipps et al., de klassieke radio-antigeen bindings assay (RABA) en de nieuw ontwikkelde Chiron competitieve ELISA voor de bepaling van anti-Hib polysaccharide (HibPs) antistof concentraties in humane sera wordt beschreven in het rapport. Voor

  10. Criterio no asintótico para el diseño de sistemas Bonus-Malus mediante GPBM

    Directory of Open Access Journals (Sweden)

    Antonio Heras Martínez

    2002-01-01

    Full Text Available El diseño de sistemas Bonus-Malus habitualmente está basado en la distribución estacionaria de la probabilidad. Se supone que una póliza ha alcanzado el estado estacionario (n0 cuando la probabilidad de que dicha póliza pertenezca a la clase Ci es constante para todo n mayor o igual que n0 . Esta distribución estacionaria de probabilidad es sencilla de calcular. Su cálculo está basado en la Teoría de Cadenas de Markov y requiere que el sistema cumpla ciertas condiciones (sea Cadena de Markov irreducible y todos sus estados sean recurrentes en tiempo esperado finito. Podría ocurrir que estas condiciones no se cumplan y en este caso no se podría utilizar la distribución estacionaria. Por otra parte, los criterios basados en la distribución estacionaria no tienen en cuenta a las pólizas nuevas en la empresa ni a las que llevan poco tiempo. Por estos motivos, se propone un nuevo criterio que tenga en cuenta la edad de las pólizas de la cartera.

  11. Seguimiento continuo del proceso de gelificacion de alcoxidos de silicio y titanio mediante ensayos reologicos

    OpenAIRE

    Rudé i Payró, Elisabet; Llorens Llacuna, Joan; Mans Teixidó, Claudi, 1948-

    2000-01-01

    En este trabajo se ha estudiado el proceso de gelificación desde un nuevo punto de vista: el seguimiento de la transición solgel mediante la evolución con el tiempo de la distribución de tiempos de relajación, que está directamente relacionada con la distribución de pesos moleculares. La técnica utilizada para la determinación de la distribución de tiempos de relajación es la reología. El proceso de gelificación se ha seguido de forma continua mediante dos ensayos reológicos distintos: (1) en...

  12. Indirect competitive ELISA based on monoclonal antibody for the detection of 5-hydroxymethyl-2-furfural in milk, compared with HPLC.

    Science.gov (United States)

    Guan, Yongguang; Wu, Xinlan; Meng, Hecheng

    2013-08-01

    In this study, a method for rapid detection of 5-hydroxymethyl-2-furfural (HMF) was investigated. Monoclonal antibody (anti-HMF) was prepared and evaluated by an indirect competitive ELISA (ic-ELISA) format. The optimized standard curve was y=-0.2097x+1.0432 [where x is the logarithm (base 10) of the values of the HMF concentration and y is the absorbance of ic-ELISA results tested at 490 nm] and the linear detection range was 0.008 to 32.768 mg/L. The percentage of cross-reactivity of HMF with 5 major furfural derivatives was less than 2.92%. Finally, the established ic-ELISA format was used to test HMF in milk, and compared with the result obtained by HPLC, which produced an error of about 0.3%. Based on the data in this experiment, we concluded that the established ic-ELISA format was reliable with a high specificity.

  13. Development and application of ELISA for the detection of IgG antibodies to lymphocytic choriomeningitis virus.

    Science.gov (United States)

    Lapošová, K; Lukáčiková, Ľ; Ovečková, I; Pastoreková, S; Rosocha, J; Kuba, D; Beňa, Ľ; Tomášková, J

    2016-06-01

    Lymphocytic choriomeningitis virus (LCMV) is a neglected human pathogen, which can cause severe illnesses in humans. The most vulnerable are the human foetus and immunosuppressed individuals. Since there is no commercially available enzyme-linked immunosorbent assay (ELISA) for the diagnosis of anti-LCMV antibodies in human sera, we developed a sandwich ELISA method detecting anti-nucleoprotein IgG antibodies, using a specific monoclonal anti-nucleoprotein antibody and cells persistently infected with LCMV strain MX as antigen. In the present study we show standardization of this ELISA protocol, determination of its clinical specificity and sensitivity and its application on 30 clinical samples from multiorgan donors. Comparison of these results to the indirect immunofluorescence antibody test (IFA) demonstrates that ELISA is more sensitive. The developed ELISA assay provides a fast, simple and efficient tool for the clinical detection of anti-nucleoprotein antibodies in human sera. PMID:27265463

  14. Development of an ELISA to detect clenbuterol in swine products using a new approach for hapten design.

    Science.gov (United States)

    Bui, Quoc Anh; Vu, Thi Huynh Han; Ngo, Vo Ke Thanh; Kennedy, Ivan R; Lee, N Alice; Allan, Robin

    2016-09-01

    This research outlines the application of an enzyme-linked immunosorbent assay (ELISA) for the analysis of clenbuterol in animal products. Our assay showed good sensitivity for clenbuterol (0.4 ng/g or 0.4 ppb) and low detection limit (0.09 ng/g or 0.09 ppb). A low cross-reactivity for other β2-agonist drugs such as salbutamol, terbutaline, and epinephrine led to formatting an ELISA kit considered to have a high specificity for clenbuterol. A survey of Ho Chi Minh City pork market was conducted as part of the validation of our ELISA. ELISA results showed a surprisingly high value of contamination. However, it will be necessary to conduct a more statistically valid replicated survey with evaluation by other instrumental methods to obtain a definite conclusion. This ELISA kit will be used to monitor growth promoter residues in Vietnam's animal products. PMID:27481170

  15. Diagnóstico de Rinotraqueítis Infecciosa Bovina mediante Inmunoperoxidasa (Diagnostic of Infectious Bovine Rhinotracheítis by means of Inmunoperoxidase

    Directory of Open Access Journals (Sweden)

    Enrique Barrera Calva

    2005-11-01

    Full Text Available El objetivo de este trabajo fue diagnosticar Rinotraqueítis Infecciosa Bovina (IBR mediante la prueba de Inmunoperoxidasa. Se utilizaron 300 muestras de suero bovino, no vacunados, de varias razas, sexo y edad, procedentes de diferentes regiones ganaderas del país. Las muestras utilizadas fueron evaluadas microscópicamente para determinar que estuvieran libres de cualquier contaminación por bacterias, hongos y no hemolisadas, inactivándose posteriormente a 56° C durante 30 minutos en baño maría y congelándose hasta el momento de desarrollarse la prueba. Los sueros se trabajaron por la técnica de suero neutralización (SN en micro placas de 96 pozos utilizando células MDBK y 200 TCID50 % de virus, la prueba de ELISA se efectuó con un KIT comercial (VETOKINOL y la prueba de Inmunoperoxidasa se realizó en micro placas de 96 pozos, utilizando como antígeno el virus de IBR cepa los Ángeles con un titulo de 10 DICC 50 % y células MDBK. No hubo diferencias estadísticas significativas entre las pruebas. La prueba de Inmunoperoxidasa pude ser utilizada para el diagnóstico cualitativo rápido, confiable y económico de anticuerpos contra IBR. The objective of this work was to diagnose Infectious Bovine Rinotraqueítis (IBR by means of the test of Inmunoperoxidase. 300 samples of bovine serum were used, not vaccinated, of several races, sex and age, coming from different cattle regions of the country. The used samples were evaluated microscopically to determine that they were free of any contamination for bacteriums, mushrooms and non hemolisadas, inactivated later on to 56° C during 30 minutes in bathroom Maria and freezing until the moment to be developed the test. The serums one worked for the technique of serum neutralization (SN in micro badges of 96 wells using cells MDBK and 200 TCID50 virus%, ELISA'S test was made with a commercial KIT (VETOQUINOL and the test of Inmunoperoxidase was carried out in micro badges of 96 wells, using

  16. Redes inalámbricas y simulación de WLAN mediante OPNET

    OpenAIRE

    Romero Kanashiro, Walter R.

    2013-01-01

    Proyecto que incluye una investigación sobre las tecnologías usadas en redes inalámbricas y su simulación mediante OPNET para su evaluación. Projecte que inclou una investigació sobre les tecnologies usades en xarxes sense fils i la seva simulació mitjançant OPNET per a la seva avaluació.

  17. Effetto dell'analgesia epidurale sulla progressione della testa fetale valutata mediante ecografia 3D

    OpenAIRE

    Arcangeli, Tiziana

    2014-01-01

    Introduzione: L'analgesia epidurale è stata messa in correlazione con l'aumento della durata del secondo stadio del travaglio e del tasso di utilizzo della ventosa ostetrica. Diversi meccanismi sono stati ipotizzati, tra cui la riduzione di percezione della discesa fetale, della forza di spinta e dei riflessi che promuovono la progressione e rotazione della testa fetale nel canale del parto. Tali parametri sono solitamente valutati mediante esame clinico digitale, costantemente riportato ...

  18. Technical note: Comparison of radial immunodiffusion and ELISA for quantification of bovine immunoglobulin G in colostrum and plasma.

    Science.gov (United States)

    Gelsinger, S L; Smith, A M; Jones, C M; Heinrichs, A J

    2015-06-01

    Historically, radial immunodiffusion (RID) has been the only method that directly measures IgG; however, recent studies have reported IgG concentrations in colostrum, milk, and plasma as measured using an ELISA. To our knowledge no comparison between RID and ELISA methods has been made for bovine colostrum or plasma. The objective of this study was to compare IgG concentrations measured by both methods in samples of bovine colostrum before and after heat treatment and bovine plasma. Concentration of IgG was quantified using a commercially available RID kit and a modified ELISA. Samples of bovine colostrum and plasma were collected from individual animals and colostrum was tested before and after heat treatment at 60°C for 30 min. All samples were tested using both methods. Pearson correlation coefficients were determined for RID and ELISA values from unheated colostrum, heat-treated colostrum, and plasma samples. Mixed models were used to determine the effect of assay on IgG measurement in colostrum and plasma and effect of heat treatment on IgG concentration in colostrum. A weak correlation was found between ELISA and RID results in plasma and unheated colostrum. Concentration of IgG was significantly lower in all sample types when measured by ELISA compared to RID. Thus, direct comparison of ELISA and RID results is not recommended. Colostrum IgG concentration significantly decreased after heat treatment as measured by ELISA, but means were not different when measured by RID. Correlation plots between colostrum values measured before and after heat treatment indicated changes in the colostrum protein matrix due to heat affected RID and ELISA assays differently. This investigation compared RID and ELISA results, but no conclusions could be drawn as to the accuracy of either assay. PMID:25841961

  19. Screening for protein-DNA interactions by automatable DNA-protein interaction ELISA.

    Directory of Open Access Journals (Sweden)

    Luise H Brand

    Full Text Available DNA-binding proteins (DBPs, such as transcription factors, constitute about 10% of the protein-coding genes in eukaryotic genomes and play pivotal roles in the regulation of chromatin structure and gene expression by binding to short stretches of DNA. Despite their number and importance, only for a minor portion of DBPs the binding sequence had been disclosed. Methods that allow the de novo identification of DNA-binding motifs of known DBPs, such as protein binding microarray technology or SELEX, are not yet suited for high-throughput and automation. To close this gap, we report an automatable DNA-protein-interaction (DPI-ELISA screen of an optimized double-stranded DNA (dsDNA probe library that allows the high-throughput identification of hexanucleotide DNA-binding motifs. In contrast to other methods, this DPI-ELISA screen can be performed manually or with standard laboratory automation. Furthermore, output evaluation does not require extensive computational analysis to derive a binding consensus. We could show that the DPI-ELISA screen disclosed the full spectrum of binding preferences for a given DBP. As an example, AtWRKY11 was used to demonstrate that the automated DPI-ELISA screen revealed the entire range of in vitro binding preferences. In addition, protein extracts of AtbZIP63 and the DNA-binding domain of AtWRKY33 were analyzed, which led to a refinement of their known DNA-binding consensi. Finally, we performed a DPI-ELISA screen to disclose the DNA-binding consensus of a yet uncharacterized putative DBP, AtTIFY1. A palindromic TGATCA-consensus was uncovered and we could show that the GATC-core is compulsory for AtTIFY1 binding. This specific interaction between AtTIFY1 and its DNA-binding motif was confirmed by in vivo plant one-hybrid assays in protoplasts. Thus, the value and applicability of the DPI-ELISA screen for de novo binding site identification of DBPs, also under automatized conditions, is a promising approach for a

  20. Automatic tracking of the constancy of the imaging chain radiographic equipment using integrated tool for dummy and evaluation software; Seguimiento automatico de la constancia de la cadena de imagen de Equipos Radiograficos mediante herramienta integrada por maniqui y software de evaluacion

    Energy Technology Data Exchange (ETDEWEB)

    Mayo, P.; Rodenas, F.; Marin, B.; Alcaraz, D.; Verdu, G.

    2011-07-01

    This paper presents an innovative tool nationwide for the automatic analysis of the constancy of the imaging chain digital radiographic equipment, both computed radiography (CR) and direct digital (DR).

  1. Neurosifilis en pacientes con infeccion por VIH. Evaluacion de la repuesta al tratamiento mediante la deteccion del ADN que codifica la proteina de membrana de 47 kDa del T pallidum en LCR por la reaccion en cadena de la polimerasa

    OpenAIRE

    Bordón Ferreyra, José M.

    2000-01-01

    Numerosos trabajos describieron un incremento de la prevalencia de la sifilis en laultima decada. La reaparicion de la sifilis fue ademas descrita asociada a presentacionesatipicas en pacientes con infeccion por VIH asi como de importantes controversias en eldiagnostico serologico y repuesta al tratamiento estandarizado. Esta informacion deriva decasos clinicos y pequenas series de pacientes con infeccion por VIH-1 en quienes seencontro importantes limitaciones en la interpretacion de la ser...

  2. Neurosífilis en pacientes con infección por VIH. Evaluación de la respuesta al tratamiento mediante la detección del ADN de la proteína de membrana de 47 kDa del T pallidum en LCR por la reacción en cadena de la polimerasa

    OpenAIRE

    Bordón Ferreyra, José M.

    2002-01-01

    Consultable des del TDX Títol obtingut de la portada digitalitzada Numerosos trabajos describieron un incremento de la prevalencia de la sifilis en laultima decada. La reaparicion de la sifilis fue ademas descrita asociada a presentacionesatipicas en pacientes con infeccion por VIH asi como de importantes controversias en eldiagnostico serologico y repuesta al tratamiento estandarizado. Esta informacion deriva decasos clinicos y pequenas series de pacientes con infeccion por VIH-1 en qu...

  3. Detección y diferenciación de Entamoeba histolytica y Entamoeba dispar mediante reacción en cadena de la polimerasa en individuos de una comunidad del Estado Zulia, Venezuela Detection and differentiation of Entamoeba histolytica and Entamoeba dispar by polymerase chain reaction in a community in Zulia State, Venezuela

    Directory of Open Access Journals (Sweden)

    Zulbey Rivero

    2009-01-01

    Full Text Available La identificación diferencial de Entamoeba histolytica y Entamoeba dispar es esencial para un tratamiento adecuado del paciente y con fines epidemiológicos. Para determinar la prevalencia de E. histolytica y E. dispar se estandarizó y aplicó un ensayo de PCR, utilizando oligonucleótidos específicos para cada especie. 204 muestras de heces de individuos de la comunidad de Santa Rosa de Agua (Municipio Maracaibo, Estado Zulia, Venezuela, fueron analizadas a través del examen directo con SSF (0,85% y lugol, concentrado de formol-éter y PCR. Al examen microscópico, 42 individuos (20,58% presentaron formas evolutivas del complejo E. histolytica/E. dispar; mientras que la técnica de PCR evidenció un total de 47 casos positivos a estas amibas; de los cuales 22 eran portadores de E. histolytica (10,78%, 16 (7,84% de E. dispar y 9 (4,41% presentaron infección mixta. No hubo diferencia significativa al relacionar las variables sexo y presencia de E. histolytica y/o E. dispar, ni con los grupos etarios. No existieron casos de estas amibas, en los menores de 2 años. La frecuencia observada de E. histolytica (31/204, demuestra el carácter endémico de la amibiasis en esta comunidad.Differential identification of Entamoeba histolytica and Entamoeba dispar is essential for both appropriate patient treatment and epidemiological purposes. To determine the prevalence of these amoeba infections in Santa Rosa de Agua (Maracaibo, Zulia State, Venezuela, a PCR assay using specific primers for each species was standardized and applied. 204 stool samples were analyzed through direct microscopic examination with SSF (0.85% and lugol, formol-ether concentration, and PCR. Under direct microscopy, 42 individuals (20.58% presented the E. histolytica/E. dispar complex. Meanwhile PCR showed 47 positive cases for these amoebas: 22 E. histolytica (10.78%, 16 E. dispar (7.84%, and 9 (4.41% mixed infections. There was no significant difference in the presence of E. histolytica and/or E. dispar according to either gender or age. There were no cases of these amoebas in children under 2 years of age. Observed frequency of E. histolytica (31/204 shows the endemic nature of amoeba infection in this community.

  4. Filamentos elásticos de elastolefina de alta durabilidad obtenidos a partir de copolímeros de bloque de etileno-octeno de baja densidad mediante la tecnología de polimerización por transferencia de cadena para aplicaciones en indumentaria de uso profesional

    OpenAIRE

    Verdú Blasco, Pau

    2010-01-01

    En la actualidad el mercado carece de prendas elásticas para uso profesional puesto que los elastanos habitualmente utilizados son inestables frente a altas temperaturas y químicos agresivos, e.g. termofijado, mantenimiento de indumentaria industrial etc. Recientemente se han venido utilizado filamentos olefínicos obtenidos a partir de copolímeros al azar de etileno-octeno (Dow XLA fibers producidos por The Dow Chemical Company). Aunque más resistentes a las temperaturas...

  5. The use of polyvinyl alcohol glutaraldehyde as solid-phase in ELISA for plague

    Directory of Open Access Journals (Sweden)

    Aureci Maria Araujo

    1996-04-01

    Full Text Available Discs of polyvinyl alcohol cross-linked with glutaraldehyde were synthesized under acid catalysis (H2SO4. Then, the antigen F1 purified from Yersinia pestis was covalently linked to this modified polymer. Afterwards, an enzyme-linked immunosorbent assay (ELISA was established for the diagnosis of plague in rabbit and human. The best conditions for the method were achieved by using 1.3 ¼g of F1 prepared in 0.067 M phosphate buffer, pH 7.2, containing 1 M NaCl (PBS; anti-IgG peroxidase conjugate diluted 6,000 times and as a blocking agent 3% w/v skim milk in PBS. The titration of positive rabbit serum according to this procedure detected antibody concentrations up to 1:12,800 times. The present method, the conventional ELISA and passive haemagglutination assay are compared.

  6. Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa

    Directory of Open Access Journals (Sweden)

    GH Oliveira

    2006-03-01

    Full Text Available This study evaluated two enzyme-linked immunosorbent assays (ELISA in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM, and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.

  7. Amperometric Detection of Bacillus anthracis Spores: A Portable, Low-Cost Approach to the ELISA

    Directory of Open Access Journals (Sweden)

    Gabriel D. Peckham

    2013-01-01

    Full Text Available Antibody-based detection assays are generally robust, a desirable characteristic for in-the-field use. However, to quantify the colorimetric or fluorescent signal, these assays require expensive and fragile instruments which are ill-suited to in-the-field use. Lateral flow devices (LFDs circumvent these barriers to portability but suffer from poor sensitivity and subjective interpretation. Here, an antibody-based method for detecting Bacillus anthracis spores via amperometric signal generation is compared to ELISA and LFDs. This amperometric immunoassay uses antibody conjugated to magnetic beads and glucose oxidase (GOX along with the electron mediator 2, 6-dichlorophenolindophenol (DCPIP for production of a measurable current from a 0.4 V bias voltage. With similar sensitivity to ELISA, the assay can be completed in about 75 minutes while being completely powered and operated from a laptop computer. Immunoassay amperometry holds promise for bringing low-cost, quantitative detection of hazardous agents to the field.

  8. FACTIBILIDAD DEL USO DE UN ELISA INDIRECTO PARA LA DETECCIÓN DE Glomus clarum

    Directory of Open Access Journals (Sweden)

    I. de la Providencia

    2004-01-01

    Full Text Available Este trabajo tuvo como objetivo evaluar la factibilidad del uso de un ensayo inmunoenzimático sobre soporte sólido (ELISA, para la detección de esporas de Glomus clarum. Para ello se inmunizaron conejos de la raza Chinchilla con extractos proteicos provenientes de dichas esporas. El suero policlonal obtenido de los animales inmunizados se enfrentó a G. clarum y a las esporas de otras especies de Glomales. Se logró detectar las esporas de G. clarum con el uso del ELISA indirecto a la dilución 1/3 000, sin reacciones cruzadas con las otras especies de Glomales utilizadas en este estudio a esa misma dilución.

  9. ELISA - The method of choice for assay of antibodies in sera of livestock

    International Nuclear Information System (INIS)

    Q fever, a coxiellosis, in animals is a worldwide zoonosis caused by Coxiella burnetii. Domestic ruminants - sheep, goats and cattle- are the main reservoirs of C. burnetii responsible for both human and livestock infections. Serology remains the major means of confirmation of clinically suspect C. burnetii infection as well as for seroepidemiological studies. A sandwich enzyme linked immunosorbent assay (ELISA) has been used to detect antibodies in different animal sera and was compared with three commonly used serological methods. Six antigenic preparations obtained by different methods from C. burnetii strain Nine Mile in phases I and II have also been compared. Our results indicate that the detection of antibodies to C. burnetii can be influenced not only by different antigenic preparations, but also by different serological methods. The advantageous sandwich ELISA with its simplicity, automation and objective reading represents the method of choice for screening great numbers of samples and for evaluating epizotoological sitiuations

  10. ELISA to study antibody responses to anthrax vaccine in cattle, sheep and goats

    International Nuclear Information System (INIS)

    An ELISA based on tri-partite toxin partially purified from Bacillus anthracis was used to determine the antibody responses of groups of cattle, sheep and goats after anthrax vaccination. All species produced detectable increases in antibody titres after vaccination with the order being cattle>sheep>goats. A second vaccination induced variable anamnestic responses depending on the species or time (6 or 22 weeks) after the primary dose. Large differences were observed between individual animals with respect to the antibody induced by the 2 vaccine doses. This observation, together with differences in vaccine quality and apparent immunogenicity may affect efficient control of anthrax outbreaks. ELISA provided a convenient method of determining the relative contribution of these factors to the protection afforded by anthrax vaccination programs. (author). 10 refs, 3 figs, 2 tabs

  11. The eLISA gravitational reference sensor and its test aboard LISA Pathfinder

    Science.gov (United States)

    Weber, William Joseph; Dolesi, Rita; Vitale, Stefano

    The upcoming LISA Pathfinder (LPF) mission represents the state-of-the-art in realizing a set of free-falling reference test masses for gravitational wave astronomy. The key hardware for achieving the smallest possible deviation from pure geodesic motion lies in the gravitational reference sensor or GRS. We present the GRS designed for achieving sub-femto-g/sqrt{mathrm{Hz}} performance with LPF and eLISA and discuss our current experimental estimates for the upcoming mission, including the latest results from the flight hardware test campaigns. Finally, we will address the LPF in-flight tests that will complete our physical model for test mass acceleration noise for eLISA and other possible experimental gravitational measurements in space.

  12. Construction of an optical test-bed for eLISA

    Science.gov (United States)

    Lieser, Maike; Fitzsimons, E.; Isleif, K.-S.; Killow, C.; Perreur-Lloyd, M.; Robertson, D.; Schuster, S.; Tröbs, M.; Veith, S.; Ward, H.; Heinzel, G.; Danzmann, K.

    2016-05-01

    In the planned eLISA mission a key part of the system is the optical bench that holds the interferometers for reading out the inter-spacecraft distance and the test mass position. We report on ongoing technology development for the eLISA optical system like the back-link between the optical benches and the science interferometer where the local beam is interfered with the received beam from the distant spacecraft. The focus will be on a setup to investigate the tilt-to-pathlength coupling in the science interferometer. To test the science interferometer in the lab a second bench providing a laser beam and a reference interferometer is needed. We present a setup with two ultra-stable low expansion glass benches and bonded optics. To suppress the tilt-to-pathlength coupling to the required level (few μm/rad) imaging optics are placed in front of the interferometer photo diodes.

  13. Evaluation of a serological Salmonella Mix-ELISA for poultry used in a national surveillance programme

    DEFF Research Database (Denmark)

    Feld, Niels Christian; Ekeroth, Lars; Gradel, K.O.;

    2000-01-01

    by Mix-ELISA and by faecal culture, and in case of a positive result in either of these a repeated, serological testing was performed, and 60 animals were organ-cultured. If one of these samplings was positive, the flock was declared salmonella infected. In a period of 3 months, 35 flocks were found......A Mix-ELISA using lipopolysaccharide antigens from Salmonella enterica serotype Enteritidis and Typhimurium? was evaluated using samples collected over time in the Danish salmonella surveillance programme for poultry. Serological samples (n = 42813) taken from broiler-breeder flocks after a year...... of bacteriological monitoring with negative results were used for calculating the flock and individual test specificities, which were 0.997 and 0.999, respectively. Layer flocks from the table egg sector were used for calculation of positive predictive values. In the survey, flocks were examined for salmonella...

  14. Agricultural production - Phase 2. Indonesia. National training course on ELISA for seradiagnosis of animal diseases (4)

    International Nuclear Information System (INIS)

    This report details and UNDP/FAO/IAEA consultancy undertaken from Monday 13 February to Saturday 25 February 1989. The purpose of the consultancy was to provide practical and theoretical training to Indonesian scientists in ELISA technology. This occurred under the program title of ''National Training Course on the Use of ELISA for Serodiagnosis of Animal Diseases, with Emphasis on Brucellosis''. The course was held in the Bacteriology Department, Research Institute for Veterinary Sciences (Balitvet), Bogor, Indonesia. The majority of the 19 participants came from the Regional Disease Investigation Centre Laboratories within Indonesia. The principal course instructor was Dr. Richard Jacobson who was assisted by Dr. Larry McClure, Dr. Susan Sutherland, Dr. Mark Eisler, Dr. Barry Patten and myself. The course concluded with a one day seminar organized by BATAN, DITKESWAN and BALITVET entitled ''Bovine Brucellosis: A Challenging Disease for Indonesia'' which was attended by approximately fifty people. Refs and tabs

  15. Evaluation of antigen and antibody ELISA's for epidemiological surveys of tsetse-transmitted trypanosomosis in cattle

    International Nuclear Information System (INIS)

    Sensitivity and specificity of the FAO/IAEA antigen-detection enzyme-linked immunosorbent assay (ELISA) kits for diagnosis of bovine trypanosomosis were investigated using sera from experimental cattle infected by tsetse challenge with cloned populations of Trypanosoma congolense (3 populations) or T. vivax (1 population). The kits are based on monoclonal antibodies that recognise internal antigens of tsetse-transmitted trypanosomes. Ten cattle were infected with each trypanosome population for at least 60 days, and in combination with uninfected cohorts (n=16) were used in a double-blind study design. Sensitivity and specificity of the tests depended on the choice of positive-negative thresholds expressed as percent positivity with respect to the median OD of 4 replicates of the strong positive reference serum provided with the kit. In general, while overall specificities were high, sensitivities of the antigen-ELISA's were poor. For example, at a cut-off of 5% positivity, the sensitivities of the antigen-ELISA's were 11% for samples (n=1162) from T. congolense infected cattle (n=30), and 24% for samples (n=283) from T. vivax infected cattle (n=10). The corresponding specificity values were 95% and 79%, respectively. There were no values of the positive-negative threshold at which both sensitivity and specificity were satisfactory. Trypanosome species-specificities of the antigen-ELISA's were also poor. Sensitivity and species-specificity of the antigen-ELISA for T. brucei infections were not investigated. The indirect ELISA for the detection of anti-trypanosomal antibodies in bovine serum was adapted for use with dried blood spots on filter paper, and standardised using a strong positive reference serum and the percent positivity system of data expression. The antibody-ELISA was evaluated in Zambia for use in epidemiological surveys of the prevalence of tsetse-transmitted bovine trypanosomosis. Known negative samples (sera, n=209; blood spots, n=466) were

  16. Cloning, Expression of apxI Gene of Actinobacillus pleuropneumoniae and Development of ELISA

    Institute of Scientific and Technical Information of China (English)

    LIU Jian-jie; HE Qi-gai; CHEN Huan-chun; WU Bin; XU Xiao-juan; LIU Jun-fa; TANG Xian-chun; BEI Wei-cheng

    2003-01-01

    Based on the published nucleotide sequence of the apxICA of Actinobacillus pleuropneumoniaein Genbank(S4074), a pair of primers were designed. A 3 640 bp(4 687 -8 326 bp)gene fragment was ampli-fied by PCR from the isolated strain of A. pleuropneumoniae serovar 1. Then, it was cloned into pMD18-T,identified by both restriction endonuclease and sequence analysis, and inserted into pET-28a expression vectorto yield the expression plasmid. SDS-PAGE result indicated expression of apxICA in BL21 (DE3), Westernblot analysis showed the protein's immunogenicity. Using the expressed protein, ELISA was established to de-tect serum antibody against ApxI. The feature of ELISA to detect highly virulent A. pleuropneumoniae strainsinfection was proved by primary clinical application.

  17. Detection of Epstein- Barr virus infection in lymphoma: ELISA and PCR method

    Directory of Open Access Journals (Sweden)

    Pourakbari B

    2010-02-01

    Full Text Available "n Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Arial; mso-bidi-theme-font:minor-bidi;} Background: Latent Epstein- Barr virus (EBV genomes are found in the malignant cells of approximately one-third of Hodgkin's lymphoma (HL cases. Detection of EBV viral DNA could potentially be used as a biomarker of disease activity. Our goal was to compare of EBV DNA detection in samples obtained from lymphoma patients versus controls."n"nMethods: One milliliter uncoagulated and 1ml coagulated blood sample for DNA extraction and serum analysis using ELISA for IgG anti EBNA-1 were obtained from 44 lymphoma patients and from 44 normal controls, respectively. EBV genome, EBNA-2, was examined from DNA extracts of paraffin embedded and blood samples using Nested PCR with type specific inner primers."n"nResults: Positive results for ELISA, Blood and biopsy PCR in study group were, 84.1%, 27.3% and 13.6%, respectively. However, these results in control group were 47.7% and 16% for ELISA and Blood PCR assays, respectively. Positive results in ELISA, Blood PCR and Biopsy PCR in Hodgkin and non-Hodgkin patients were found in 21(84%, 6(24%, 4(16% and 16(84.2%, 6(31.6%, 2(10.5% of specimens, respectively. No significant differences in EBV detection were found between these two patient groups (p values for ELISA, Blood PCR

  18. Methodology for determination of plasma cortisol in fish using Competitive Enzyme-Linked Immunosorbent Assay (ELISA)

    DEFF Research Database (Denmark)

    Velasco-Santamaría, Yohana M.; Cruz-Casallas, Pablo E.

    2007-01-01

    Objective. To determine plasma cortisol procedure in fish using competitive enzymelinked immunosorbent assay (ELISA). Materials and methods. Two plasma samples of juveniles rainbow trout Oncorhynchus mykiss were analized by using ELISA human kit for cortisol assay. For standard curve calibration...... seven standard solutions of cortisol in human plasma (0, 20, 50, 100, 200, 400 and 800 ng.ml-1) were used. For the recovery test 50, 100 and 200 ng.ml-1 standard solutions of cortisol were used; for the linearity test four dilutions of the fish plasma samples (1/2, 1/4, 1/8 and 1/16) were prepared...... procedure was assessed previously. The recovery and linearity percentages, the standard curve and parallelism were determined. Results. The standard curve showed a high correlation coefficient (r2 = 0.998). The cortisol concentration of two samples fluctuated between 64 and 72 ng.ml-1. Only the 200 ng.ml-1...

  19. LDRD final report on microencapsulated immunoreagents for development of one-step ELISA

    Energy Technology Data Exchange (ETDEWEB)

    Henderson, C.C.; Singh, A.K.

    1997-08-01

    Microencapsulation of biological macromolecules was investigated as a method for incorporating the necessary immunoreagents into an improved enzyme-linked immunosorbant assay (ELISA) package that would self-develop. This self-contained ELISA package would eliminate the need for a trained technician to perform multiple additions of immunoreagent to the assay. Microencapsulation by insolution drying was selected from the many available microencapsulation methods, and two satisfactory procedures for microencapsulation of proteins were established. The stability and potential for rapid release of protein from these microencapsulates was then evaluated. The results suggest that the chosen method for protein entrapment produces microcapsules with a considerable amount of protein in the walls making these particular microcapsules unsuitable for their intended use.

  20. Study of H-Y antigen in abnormal sex determination with monoclonal antibody and an ELISA.

    Science.gov (United States)

    Moreira-Filho, C A; Wachtel, S S

    1985-03-01

    A newly developed enzyme-linked immunosorbent assay (ELISA) has been applied to the study of H-Y antigen in cases of XY, XYY, and X,dicY gonadal dysgenesis, testicular feminization syndrome, XXXXY syndrome, and XX true hermaphroditism. Monoclonal H-Y antibody was absorbed with cells from each of eight patients and from normal male and female controls, and then reacted with a plated antigen source in a system subsuming the addition of biotinylated secondary antibody, avidin-biotin-enzyme complex and substrate, and thereby the generation of a color. Positive absorption decreased the reaction, and this allowed sensitive measurement of H-Y phenotype in an electronic optical density reader. The ELISA obviates many of the technical difficulties encountered in complement-mediated cytotoxicity systems and can be used in the study of clinical cases of aberrant sex determination and in the evaluation of current models of the genetics of sex determination.

  1. Comentario sobre la ponencia: “La experiencia de incorporar a las procesadoras en la cadena de lácteos en Occidente: el caso de la Cuenta Reto del Milenio”, presentada el 20 de abril de 2012 por Sylvia Torres.

    Directory of Open Access Journals (Sweden)

    Anielka Pérez

    2012-08-01

    Full Text Available La conferencia brindada por Sylvia Torres sobre la participación de las mujeres en la cadena productiva del sector lácteo en León y Chinandega resultó sugestiva. La ponente presentó la experiencia de cómo las mujeres se vinculan al sector ganadero, el aporte que hacen y cómo éste casi nunca se toma en cuenta en los proyectos de desarrollo.

  2. Rapid Enhanced MM3-COPRO ELISA for Detection of Fasciola Coproantigens.

    Directory of Open Access Journals (Sweden)

    Victoria Martínez-Sernández

    2016-07-01

    Full Text Available ELISA-based methods of detecting Fasciola cathepsins in feces are powerful techniques for diagnosing infections by F. hepatica and F. gigantica. In the last decade, the in-house MM3-COPRO ELISA and its commercial version BIO K 201 (BIO X Diagnostics, Belgium have been recognized as useful tools for detecting early infections by such trematodes and for monitoring the efficacy of anthelmintic treatments in human and animal species, as they provide some advantages over classic fecal egg counts. However, the sensitivity of MM3-COPRO ELISA can sometimes be compromised by the high variability in the concentration of cathepsins in fecal samples throughout the biological cycle of Fasciola (mainly in cattle and by differences in the between-batch performance of peroxidase-labeled anti-mouse IgG polyclonal antibodies. To prevent such problems, we investigated whether the incorporation of a commercial streptavidin-polymerized horseradish peroxidase conjugate, in order to reveal bound biotinylated monoclonal antibody MM3, can improve the sensitivity of the MM3-COPRO ELISA. We observed that inclusion of this reagent shifted the previous detection limit of the assay from 0.6 ng/mL to 150 pg/mL and that the modified test is able to identify infection in cows harboring only one fluke. Moreover, we demonstrated that maximal OD values can be achieved with short incubations (30 min each step at RT with shaking, rather than standard incubations, which significantly accelerates the diagnostic procedure. Finally, we did not find a significant correlation between coproantigen concentration and parasite burden in cattle, which may be due to the low parasite burden (1-10 adult flukes of the animals used in the present study. As the usefulness of the classic MM3-COPRO test for detecting animal and human infections has already been demonstrated, it is expected that the improvements reported in this study will add new insights into the diagnosis and control of fasciolosis.

  3. High specificity of a novel Zika virus ELISA in European patients after exposure to different flaviviruses.

    Science.gov (United States)

    Huzly, Daniela; Hanselmann, Ingeborg; Schmidt-Chanasit, Jonas; Panning, Marcus

    2016-04-21

    The current Zika virus (ZIKV) epidemic in the Americas caused an increase in diagnostic requests in European countries. Here we demonstrate high specificity of the Euroimmun anti-ZIKV IgG and IgM ELISA tests using putative cross-reacting sera of European patients with antibodies against tick-borne encephalitis virus, dengue virus, yellow fever virus and hepatitis C virus. This test may aid in counselling European travellers returning from regions where ZIKV is endemic. PMID:27126052

  4. Automated type specific ELISA probe detection of amplified NS3 gene products of dengue viruses.

    OpenAIRE

    Chow, V T; Yong, R Y; Ngoh, B L; Chan, Y. C.

    1997-01-01

    AIM: To apply an automated system of nucleic acid hybridisation coupled with the enzyme linked immunosorbent assay (ELISA) for the type specific detection of amplification products of dengue viruses. METHODS: Non-structural 3 (NS3) gene targets of reference strains of all four dengue and other flaviviruses, as well as dengue patient viraemic sera, were subjected to reverse transcription and polymerase chain reaction using consensus and dengue type specific primers and digoxigenin-11-dUTP labe...

  5. Conglutinating complex fixation test (CCFR) as a method alternate to enzyme linked immunosorbent assay (ELISA)

    International Nuclear Information System (INIS)

    An enzyme linked immunosorbent assay (ELISA) has proved to be the most versatile and sensitive method for the serological diagnosis of infectious animal diseases. However, other assays offer similar advantages. This has been shown by our research through the development of a conglutinating complex fixation test (CCFT). This is analogous to the complement fixation test (CFT) and is based on the phenomenon of conglutination. The limiting factor in this test is the source in cattle blood

  6. Detection of Foot-and-mouth Disease Serotype O by ELISA Using a Monoclonal Antibody

    OpenAIRE

    Chen, Hao-tai; Peng, Yun-hua; ZHANG, YONG-GUANG; Liu, Xiang-tao

    2012-01-01

    An ELISA assay with monoclonal antibody (MELISA) was used to type serotype O of foot-and-mouth disease virus (FMDV). All FMDV serotype O reference strains were positive by MELISA, while other viruses such as FMDV serotypes Asia 1, C, and A and classical swine fever virus, swine vesicular disease virus, and porcine reproductive and respiratory syndrome virus remained negative. Furthermore, FMDV serotype O positive samples were able to be detected by MELISA. This assay may be particularly suita...

  7. Development of sandwich ELISAs for the detection of aromatic diisocyanate adducts

    OpenAIRE

    Lemons, Angela R.; Bledsoe, Toni A.; Siegel, Paul D.; Beezhold, Donald H.; Brett J Green

    2013-01-01

    Diisocyanates (dNCOs) are highly reactive low molecular weight chemicals commonly used in the manufacturing industry. Occupational exposures to dNCOs have been shown to elicit allergic sensitization and occupational asthma. Among the most commonly used dNCOs in industry are the aromatic dNCOs, toluene diisocyanate (TDI) and methylene diphenyl diisocyanate (MDI). This study aimed to develop enzyme linked immunosorbent assays (ELISA) utilizing aromatic dNCO-specific monoclonal antibodies (mAbs)...

  8. Detection of ruminant meat and bone meal in feeds by sandwich ELISA with monoclonal antibodies.

    Science.gov (United States)

    Yamamoto, Takayuki; Kato, Masatoshi; Endo, Kiwamu; Kotoura, Satoshi; Takeda, Zenya

    2016-01-01

    A sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) using two monoclonal antibodies directed against a synthetic peptide with an amino-acid sequence related to the C-terminus of bovine myoglobin and the whole molecule of sodium dodecyl sulphate (SDS)-denatured bovine myoglobin was adapted for detecting bovine myoglobin in contaminated feeds. The ELISA employed bovine meat extract of a known myoglobin concentration as a calibration standard and had an limit of detection (LOD) of 3.54 ng/ml and an limit of quantification (LOQ) of 11.0 ng/ml corresponding to 0.022% and 0.067% (wt/wt) bovine meat-and-bone-meal (MBM) mixed in 20-fold-diluted feed extracts, respectively. A cut-off threshold of 20.6 ng/ml bovine myoglobin was set to simplify ELISA and facilitate quick assessment of test results without a tedious calibration process. The ELISA was able to detect bovine MBM in artificially prepared model feeds, mixed botanical feeds, mixed botanical feeds with skimmed milk, fish meal, pork meal and pork/chicken meal at 0.1% (wt/wt). It was also able to detect sheep MBM in test feeds, but showed no reactivity to swine MBM, chicken MBM, skimmed milk or gelatine of bovine origin. The advantages of this method are the quick and easy extraction protocol of proteins from test feeds, using 100 mM sodium sulphide and 0.6% sodium dodecyl sulphate in the extraction solution and the effective detection of bovine and sheep MBM at 0.1% (wt/wt).

  9. Detection of expressed IL-32 in human stomach cancer using ELISA and immunostaining.

    Science.gov (United States)

    Seo, Eun-Hee; Kang, Jeongwoo; Kim, Ki-Hong; Cho, Min-Chul; Lee, Sojung; Kim, Hee-Jong; Kim, Jung-Hee; Kim, Eun-Jin; Park, Dong-Ki; Kim, Soo-Hyun; Choi, Yang Kyu; Kim, Jin Man; Hong, Jin Tae; Yoon, Do-Young

    2008-09-01

    Interleukin (IL)-32 is a recently identified proinflammatory cytokine that is one of the IL-18 inducible genes, and plays an important role in autoimmune and inflammatory diseases. We produced antibodies against IL-32 and studied the expression of IL-32 in human stomach cancer. We detected IL-32 secreted from K-562 cells that werw stably transfected with IL-32 and in the sera of stomach cancer patients, by a sandwich ELISA using a monoclonal antibody KU32-52 and a polyclonal antibody. In order to optimize a sandwich immunoassay, recombinant IL-32alpha was added, followed by the addition of a biotinylated KU32-52 into microtiter plate wells precoated with a goat anti-IL-32 antibody. The bound biotinylated KU32-52 was probed with a streptavidin conjugated to HRP. This sandwich ELISA was highly specific and had a minimal detection limit of 80 pg/ml (mean+/-SD of zero calibrator) and measuring up to 3,000 pg/ml. This ELISA showed no cross-reaction with other cytokines such as hIL-1alpha, hIL-1beta, hIL-2, hIL-6, hIL-8, hIL-10, hIL-18, and hTNF-alpha. Intra-assay coefficients of variation were 18.5% to 4.6% (n=10), and inter-assay coefficients were 23% to 9% (n=10). The average IL-32 level in the sera of 16 stomach cancer patients (189 pg/ml) was higher than that of 12 healthy control men (109 pg/ml). Our results indicate that serum IL-32 level can be detected by using an established ELISA, and that this immunoassay and mAb KU32-09 specific for immunohistochemistry can be used in the detection of expressed and secreted IL- 32 in stomach cancer patients. PMID:18852519

  10. Detection of peste des petits ruminants virus antigen using immunofiltration and antigen-competition ELISA methods.

    Science.gov (United States)

    Raj, G Dhinakar; Rajanathan, T M C; Kumar, C Senthil; Ramathilagam, G; Hiremath, Geetha; Shaila, M S

    2008-06-22

    Peste des petits ruminants (PPR) is one of the most economically important diseases affecting sheep and goats in India. An immunofiltration-based test has been developed using either mono-specific serum/monoclonal antibodies (mAb) prepared against a recombinant truncated nucleocapsid protein of rinderpest virus (RPV) cross-reactive with PPR virus. This method consists of coating ocular swab eluate from suspected animals onto a nitrocellulose membrane housed in a plastic module, which is allowed to react with suitable dilutions of a mAb or a mono-specific polyclonal antibody. The antigen-antibody complex formed on the membrane is then detected by protein A-colloidal gold conjugate, which forms a pink colour. In the immunofiltration test, concordant results were obtained using either PPRV mAb or mono-specific serum. Another test, an antigen-competition ELISA which relies on the competition between plate-coated recombinant truncated 'N' protein of RPV and the PPRV 'N' protein present in ocular swab eluates (sample) for binding to the mono-specific antibody against N protein of RPV (in liquid phase) was developed. The cut-off value for this test was established using reverse transcription polymerase chain reaction (RT-PCR) positive and negative oculo-nasal swab samples. Linear correlation between percent inhibition (PI) values in antigen-competition ELISA and virus infectivity titres was 0.992. Comparison of the immunofiltration test with the antigen-competition ELISA yielded a sensitivity of 80% and specificity of 100%. These two tests can serve as a screening (immunofiltration) and confirmatory (antigen-competition ELISA) test, respectively, in the diagnosis of PPR in sheep or goats. PMID:18182256

  11. Validation of antibody ELISA for use in combination with parasitological tests in monitoring trypanosomosis control programmes

    International Nuclear Information System (INIS)

    The available techniques for trypanosomosis diagnosis, surveillance and control have failed to provide an effective control of the disease when used in isolation. However, when used in combination the techniques might provide a better alternative for a sustainable disease control strategy. Antigen-detection ELISA was validated and established at ADRI through support of the Joint FAO/IAEA Division, but still required perfection in order to meet the requirements for which it was intended (detection of current infection and species specificity). The primary objective of the present study was to evaluate the trypanosomal antibody-detection ELISA (Ab-ELISA) kit produced by the International Atomic Energy Agency (IAEA) Vienna, Austria, to supplement conventional diagnostic techniques in trypanosomosis surveillance and control. Three-hundred sixty cattle from selected herds in the Tanga region of Tanzania were bled to obtain buffy-coat and serum samples in a study of the prevalence of trypanosomosis using parasitological and serological techniques. The prevalence of trypanosomosis, by means of parasitological techniques, in Korogwe, Muheza, Tanga and Pangani districts of Tanga region, varied from 0-18.2%. The IAEA Ab-ELISA kit, showed a 97.2% specificity for Trypanosoma congolense and T. vivax antibodies, at a cut-off point of 30.0% positivity (PP), out of 160 sera from a trypanosomosis-negative population; and a 94.5% sensitivity for the same antibodies and cut-off point, out of 200 sera from trypanosomosis-buffy-coat-positive samples. Packed red cell volume (PCV) values for a trypanosomosis-negative population were much higher than those from cattle from trypanosomosis-endemic areas: 77.5% of samples from a disease-free area showed PCV values higher than 30.0, while only 15% of the samples from endemic areas were above 30.0. (author)

  12. Development and application of an indirect ELISA for the detection of antibodies to novel duck reovirus.

    Science.gov (United States)

    Yun, Tao; Chen, Haipeng; Yu, Bin; Zhang, Cun; Chen, Liu; Ni, Zheng; Hua, Jionggang; Ye, Weicheng

    2015-08-01

    A novel duck reovirus (N-DRV) disease emerged in China in 2000 and it has become an epidemic genotype. A test for detection of virus-specific antibodies in serum samples would be useful for epidemiological investigations. Currently, Currently, serological assays for N-DRV diagnosis are not available. A test for detection of virus-specific antibodies in serum samples would be useful for epidemiological investigations. In this study, a highly sensitive and specific indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to N-DRV was developed. The outer capsid (σC) of N-DRV was cloned and expressed in Escherichia coli as a coating antigen. The antigen concentration and serum dilution were optimized using a checkerboard titration. Furthermore, the specificity of σC-ELISA assay was confirmed by cross checking with other duck viral pathogens. In comparison with the western blot, the sensitivity and specificity of the σC-ELISA was 92.6% and 88.9%, respectively, and agreement of two tests was excellent with κ value of 0.786 (p < 0.05). A serological survey was performed using the assay on serum samples from different age and species of duck flocks in the Zhejiang and Jiangsu Province, China. The seropositive rate of the 1209 serum samples was 57.7%. In conclusion, the developed σC-ELISA assay is a very specific and sensitive test that will be useful for large-scale serological survey in N-DRV infection and monitoring antibodies titers against N-DRV.

  13. Detection of Trichinella infection in slaughter horses by artificial digestion, ELISA and PCR

    OpenAIRE

    Viveros N.; Arriaga C.; Banda V.; Ortega-Pierres M.G.; Yépez-Mulia L.

    2001-01-01

    In this study we compared the sensitivity of molecular, serologic and parasitologic methods for diagnosis of equine trichinellosis in two abattoirs, one rural and one federal inspection type. Diaphragm muscle samples were obtained from 170 slaughter horses and examined by artificial digestion and PCR. Serum samples from these horses were also analyzed by ELISA. No Trichinella muscle larvae were detected by artificial digestion. However, specific antibodies against Trichinella were detected in...

  14. PrPSc detection in formalin-fixed paraffin-embedded tissue by ELISA

    OpenAIRE

    Eric M Nicholson; Greenlee, Justin J; Hamir, Amir N

    2011-01-01

    Background Formalin-fixed paraffin-embedded tissue is regularly employed in the diagnosis of transmissible spongiform encephalopathies (TSE) by immunohistochemistry (IHC), the standard by which all other TSE diagnostic protocols are judged. While IHC affords advantages over diagnostic approaches that typically utilize fresh or frozen tissue, such as Western blot and ELISA, the process of fixing, staining, and analyzing individual sections by hand does not allow for rapid or high throughput sc...

  15. A novel ELISA for measuring CD36 protein in human adipose tissue

    OpenAIRE

    Allred, Carolyn C.; Krennmayr, Thomas; Koutsari, Christina; Zhou, Lianzhen; Ali, Asem H.; Jensen, Michael D.

    2011-01-01

    CD36 is a transmembrane protein present in many tissues that is believed to facilitate inward fatty acid transport. Western blotting is the most widely used method to measure tissue CD36 protein content, but it is time consuming, technically demanding, and semiquantitative. To more precisely measure adipose tissue CD36 content we developed an enzyme linked immunosorbent assay (ELISA) after establishing that: 1) the anti-CD36 antibodies gave a single distinct band on traditional Western blots,...

  16. Rapid Enhanced MM3-COPRO ELISA for Detection of Fasciola Coproantigens

    Science.gov (United States)

    Martínez-Sernández, Victoria; Orbegozo-Medina, Ricardo A.; González-Warleta, Marta; Mezo, Mercedes; Ubeira, Florencio M.

    2016-01-01

    ELISA-based methods of detecting Fasciola cathepsins in feces are powerful techniques for diagnosing infections by F. hepatica and F. gigantica. In the last decade, the in-house MM3-COPRO ELISA and its commercial version BIO K 201 (BIO X Diagnostics, Belgium) have been recognized as useful tools for detecting early infections by such trematodes and for monitoring the efficacy of anthelmintic treatments in human and animal species, as they provide some advantages over classic fecal egg counts. However, the sensitivity of MM3-COPRO ELISA can sometimes be compromised by the high variability in the concentration of cathepsins in fecal samples throughout the biological cycle of Fasciola (mainly in cattle) and by differences in the between-batch performance of peroxidase-labeled anti-mouse IgG polyclonal antibodies. To prevent such problems, we investigated whether the incorporation of a commercial streptavidin-polymerized horseradish peroxidase conjugate, in order to reveal bound biotinylated monoclonal antibody MM3, can improve the sensitivity of the MM3-COPRO ELISA. We observed that inclusion of this reagent shifted the previous detection limit of the assay from 0.6 ng/mL to 150 pg/mL and that the modified test is able to identify infection in cows harboring only one fluke. Moreover, we demonstrated that maximal OD values can be achieved with short incubations (30 min each step) at RT with shaking, rather than standard incubations, which significantly accelerates the diagnostic procedure. Finally, we did not find a significant correlation between coproantigen concentration and parasite burden in cattle, which may be due to the low parasite burden (1–10 adult flukes) of the animals used in the present study. As the usefulness of the classic MM3-COPRO test for detecting animal and human infections has already been demonstrated, it is expected that the improvements reported in this study will add new insights into the diagnosis and control of fasciolosis. PMID:27438470

  17. The use of ELISAs for monitoring exposure of pig herds to Brachyspira hyodysenteriae

    Directory of Open Access Journals (Sweden)

    Song Yong

    2012-01-01

    Full Text Available Abstract Background Swine dysentery (SD, a mucohaemorrhagic diarrhoeal disease of pigs, results from infection of the large intestine with the spirochaete Brachyspira hyodysenteriae. ELISA systems using whole spirochaete cells (WC and the B. hyodysenteriae outer membrane lipoprotein Bhlp29.7 previously have been established as potential diagnostic tools for SD. However, their true value in identifying infected herds remains unclear. The present study aimed to compare the performance of whole-cell and Bhlp29.7 based ELISAs in detecting specific immunoglobulin class IgG and IgM to B. hyodysenteriae in growing pigs, and additionally evaluated whether meat juice could serve as a source of specific antibodies. Results Levels of circulating IgG and IgM reacting with WC spirochaete preparations and recombinant Bhlp29.7 peaked 4-6 weeks post-infection in the experimentally challenged pigs, and remained elevated in the present study. In a cohort of pigs on an infected farm levels of antibody directed against both antigens showed a progressive increase with time. However, other than for the level of IgG against WC antigen, a significant increase in antibody levels also was observed in a cohort of pigs on a non-infected farm. In addition, assays using meat juice had 100% specificity and equivalent sensitivity to those based on serum, and likewise the best performance was achieved using the WC IgG ELISA. Conclusions IgG ELISAs using either WC or Bhlp29.7 as plate-coating antigens were shown to be useful for monitoring the dynamics of B. hyodysenteriae infection in grower pigs. Of the two antigens, the WC preparation tended to give better discrimination between pigs from infected and non-infected farms. Testing of meat juice was shown to have potential for identifying infected herds.

  18. Evaluation of a blocking ELISA for the detection of antibodies against Lawsonia intracellularis in pig sera

    Directory of Open Access Journals (Sweden)

    Merza Malik

    2011-04-01

    Full Text Available Abstract Background Lawsonia intracellularis is a common cause of chronic diarrhoea and poor performance in young growing pigs. Diagnosis of this obligate intracellular bacterium is based on the demonstration of the microbe or microbial DNA in tissue specimens or faecal samples, or the demonstration of L. intracellularis-specific antibodies in sera. The aim of the present study was to evaluate a blocking ELISA in the detection of serum antibodies to L. intracellularis, by comparison to the previously widely used immunofluorescent antibody test (IFAT. Methods Sera were collected from 176 pigs aged 8-12 weeks originating from 24 herds with or without problems with diarrhoea and poor performance in young growing pigs. Sera were analyzed by the blocking ELISA and by IFAT. Bayesian modelling techniques were used to account for the absence of a gold standard test and the results of the blocking ELISA was modelled against the IFAT test with a "2 dependent tests, 2 populations, no gold standard" model. Results At the finally selected cut-off value of percent inhibition (PI 35, the diagnostic sensitivity of the blocking ELISA was 72% and the diagnostic specificity was 93%. The positive predictive value was 0.82 and the negative predictive value was 0.89, at the observed prevalence of 33.5%. Conclusion The sensitivity and specificity as evaluated by Bayesian statistic techniques differed from that previously reported. Properties of diagnostic tests may well vary between countries, laboratories and among populations of animals. In the absence of a true gold standard, the importance of validating new methods by appropriate statistical methods and with respect to the target population must be emphasized.

  19. Antigen capture ELISA for the heat shock protein (hsp60) of Chlamydia trachomatis.

    OpenAIRE

    Horner, P J; Ali, M.; Parker, D.; Weber, J. N.; Taylor-Robinson, D.; McClure, M O

    1996-01-01

    AIMS: To develop an indirect ELISA using the heat shock protein (hsp60) of Chlamydia trachomatis as antigen. METHODS: The hsp60 gene was amplified by PCR, expressed in the vector pDEV-107 and transformed into Escherichia coli. The recombinant protein, expressed as a beta-galactosidase fusion product, was captured onto a solid phase using a monoclonal antibody directed against beta-galactosidase. Following incubation with goat anti-human antibody conjugated to peroxidase and colour development...

  20. Use of an enzyme-linked immunosorbent assay (Elisa) for the determination of HSP 70

    International Nuclear Information System (INIS)

    It has been suggested that HSP 70 is a good indicator to quantify thermotolerance. The authors developed an enzyme-linked immunosorbent assay (Elisa) for measuring the total amount of HSP 70 in mammalian cells. Microtitration plates were incubated with various concentrations of cell lysate in PBS. Milk proteins were used to block non-specific protein binding sites in the wells. Monoclonal antibody against purified HSP 70 raised in a mouse was added, followed by peroxidase conjugated goat-anti-mouse antibody. After washing, the bound peroxidase activity was measured colorimetrically to calculate the original amount of HSP 70 antigen. Kinetic studies indicate that the linear range of the assay corresponds to the amount of HSP 70 from 103-104 HA-1 cells (0.1-2.0 μg cytosolic proteins in each well). Elisa is a highly sensitive method for quantifying HSP 70 and can be easily used to measure the total amount of HSP 70 instead of the synthesis rate of HSP 70 by pulse radiolabeling. The authors are currently developing the sensitive Elisa system for measurement of thermotolerance in human tumors. A comparison of measurement of HSP 70 by pulse labeling the cells with 35S-methionine and by Elisa has been done in HA-1 cells. HA-1 cells were exposed to 450C, 10 min, then incubated at 370C for 0-24 h. These results show that the rate of HSP synthesis increased several fold, although the total amount of HSP 70 is increased less than 2 fold during thermotolerance induction. These results are consistent with data in the literature obtained by other techniques